LOCUS       DAZGCQ010000001       117892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000001 DAZGCQ010000000
VERSION     DAZGCQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.001"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHK07_000001"
     CDS             complement(<1..617)
                     /locus_tag="ACHK07_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8100400.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHK07_000002"
     CDS             complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHK07_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO8100401.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHK07_000003"
     CDS             complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHK07_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO8100402.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHK07_000004"
     CDS             complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHK07_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO8100403.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHK07_000005"
     CDS             complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHK07_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO8100404.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHK07_000006"
     CDS             complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHK07_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO8100405.1"
                     /translation="MKRIAFVFSTVPHGTAVGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHK07_000007"
     CDS             complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHK07_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO8100406.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHK07_000008"
     CDS             complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHK07_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8100407.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHK07_000009"
     CDS             complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHK07_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8100408.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6928..7146
                     /gene="slyX"
                     /locus_tag="ACHK07_000010"
     CDS             6928..7146
                     /gene="slyX"
                     /locus_tag="ACHK07_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO8100409.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHK07_000011"
     CDS             complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHK07_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8100410.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHK07_000012"
     CDS             complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHK07_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO8100411.1"
                     /translation="MAIRKRFIAGAKCPVCQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHK07_000013"
     CDS             complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHK07_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO8100412.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHK07_000014"
     CDS             complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHK07_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO8100413.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10577..12490
                     /gene="yheS"
                     /locus_tag="ACHK07_000015"
     CDS             10577..12490
                     /gene="yheS"
                     /locus_tag="ACHK07_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8100414.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12490..13512
                     /gene="yheT"
                     /locus_tag="ACHK07_000016"
     CDS             12490..13512
                     /gene="yheT"
                     /locus_tag="ACHK07_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8100415.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13506..13724
                     /gene="yheU"
                     /locus_tag="ACHK07_000017"
     CDS             13506..13724
                     /gene="yheU"
                     /locus_tag="ACHK07_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO8100416.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13778..14647
                     /gene="prkB"
                     /locus_tag="ACHK07_000018"
     CDS             13778..14647
                     /gene="prkB"
                     /locus_tag="ACHK07_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO8100417.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHK07_000019"
     CDS             complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHK07_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO8100418.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15408..16040
                     /gene="crp"
                     /locus_tag="ACHK07_000020"
     CDS             15408..16040
                     /gene="crp"
                     /locus_tag="ACHK07_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO8100419.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHK07_000021"
     CDS             16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHK07_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO8100420.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTAPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRIIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKT"
     gene            complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHK07_000022"
                     /gene_synonym="dapC"
     CDS             complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHK07_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO8100421.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHK07_000023"
     CDS             complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHK07_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO8100422.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHK07_000024"
     CDS             complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHK07_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO8100423.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHK07_000025"
     CDS             complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHK07_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO8100424.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHK07_000026"
                     /gene_synonym="rotA"
     CDS             complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHK07_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO8100425.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHK07_000027"
     CDS             21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHK07_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO8100426.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23299..25842
                     /gene="nirB"
                     /locus_tag="ACHK07_000028"
     CDS             23299..25842
                     /gene="nirB"
                     /locus_tag="ACHK07_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO8100427.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLIGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25839..26165
                     /gene="nirD"
                     /locus_tag="ACHK07_000029"
     CDS             25839..26165
                     /gene="nirD"
                     /locus_tag="ACHK07_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO8100428.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26291..27097
                     /gene="nirC"
                     /locus_tag="ACHK07_000030"
     CDS             26291..27097
                     /gene="nirC"
                     /locus_tag="ACHK07_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO8100429.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27116..28489
                     /gene="cysG"
                     /locus_tag="ACHK07_000031"
     CDS             27116..28489
                     /gene="cysG"
                     /locus_tag="ACHK07_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO8100430.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28741..28908
                     /gene="yhfL"
                     /locus_tag="ACHK07_000032"
     CDS             28741..28908
                     /gene="yhfL"
                     /locus_tag="ACHK07_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO8100431.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29204..30541
                     /gene="frlA"
                     /locus_tag="ACHK07_000033"
     CDS             29204..30541
                     /gene="frlA"
                     /locus_tag="ACHK07_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO8100432.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAVTAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30562..31584
                     /gene="frlB"
                     /locus_tag="ACHK07_000034"
     CDS             30562..31584
                     /gene="frlB"
                     /locus_tag="ACHK07_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO8100433.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31634..32461
                     /gene="frlC"
                     /locus_tag="ACHK07_000035"
     CDS             31634..32461
                     /gene="frlC"
                     /locus_tag="ACHK07_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO8100434.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            32461..33246
                     /gene="frlD"
                     /locus_tag="ACHK07_000036"
     CDS             32461..33246
                     /gene="frlD"
                     /locus_tag="ACHK07_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO8100435.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33346..34077
                     /gene="frlR"
                     /locus_tag="ACHK07_000037"
                     /gene_synonym="yhfR"
     CDS             33346..34077
                     /gene="frlR"
                     /locus_tag="ACHK07_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO8100436.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNQRH"
     gene            complement(34237..35322)
                     /gene="yhfS"
                     /locus_tag="ACHK07_000038"
     CDS             complement(34237..35322)
                     /gene="yhfS"
                     /locus_tag="ACHK07_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8100437.1"
                     /translation="MKTFPLQSLTLNEAQQKQFALVDTICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RLAGIPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAHALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35334..36638)
                     /gene="yhfT"
                     /locus_tag="ACHK07_000039"
     CDS             complement(35334..36638)
                     /gene="yhfT"
                     /locus_tag="ACHK07_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO8100438.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDACGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFIPA"
     gene            complement(36650..37003)
                     /gene="yhfU"
                     /locus_tag="ACHK07_000040"
     CDS             complement(36650..37003)
                     /gene="yhfU"
                     /locus_tag="ACHK07_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO8100439.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(37014..37892)
                     /gene="php"
                     /locus_tag="ACHK07_000041"
     CDS             complement(37014..37892)
                     /gene="php"
                     /locus_tag="ACHK07_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO8100440.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYSFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37889..39115)
                     /gene="yhfW"
                     /locus_tag="ACHK07_000042"
     CDS             complement(37889..39115)
                     /gene="yhfW"
                     /locus_tag="ACHK07_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8100441.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFTTHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVTTQIGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39116..40279)
                     /gene="yhfX"
                     /locus_tag="ACHK07_000043"
     CDS             complement(39116..40279)
                     /gene="yhfX"
                     /locus_tag="ACHK07_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO8100442.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(40363..40725)
                     /gene="yhfY"
                     /locus_tag="ACHK07_000044"
     CDS             complement(40363..40725)
                     /gene="yhfY"
                     /locus_tag="ACHK07_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO8100443.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSTHWPAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40742..41647)
                     /gene="yhfZ"
                     /locus_tag="ACHK07_000045"
     CDS             complement(40742..41647)
                     /gene="yhfZ"
                     /locus_tag="ACHK07_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO8100444.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTTLGLEATPLTDDPRFLQATEAVVLTRVDDYPIQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41910..42914)
                     /gene="trpS"
                     /locus_tag="ACHK07_000046"
     CDS             complement(41910..42914)
                     /gene="trpS"
                     /locus_tag="ACHK07_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO8100445.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42907..43665)
                     /gene="gph"
                     /locus_tag="ACHK07_000047"
     CDS             complement(42907..43665)
                     /gene="gph"
                     /locus_tag="ACHK07_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO8100446.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43658..44335)
                     /gene="rpe"
                     /locus_tag="ACHK07_000048"
     CDS             complement(43658..44335)
                     /gene="rpe"
                     /locus_tag="ACHK07_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO8100447.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44353..45189)
                     /gene="dam"
                     /locus_tag="ACHK07_000049"
     CDS             complement(44353..45189)
                     /gene="dam"
                     /locus_tag="ACHK07_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8100448.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45296..46582)
                     /gene="damX"
                     /locus_tag="ACHK07_000050"
     CDS             complement(45296..46582)
                     /gene="damX"
                     /locus_tag="ACHK07_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO8100449.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRLARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46674..47762)
                     /gene="aroB"
                     /locus_tag="ACHK07_000051"
     CDS             complement(46674..47762)
                     /gene="aroB"
                     /locus_tag="ACHK07_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO8100450.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47819..48340)
                     /gene="aroK"
                     /locus_tag="ACHK07_000052"
     CDS             complement(47819..48340)
                     /gene="aroK"
                     /locus_tag="ACHK07_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO8100451.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48741..49979)
                     /gene="hofQ"
                     /locus_tag="ACHK07_000053"
     CDS             complement(48741..49979)
                     /gene="hofQ"
                     /locus_tag="ACHK07_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO8100452.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSTKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49891..50295)
                     /gene="hofP"
                     /locus_tag="ACHK07_000054"
     CDS             complement(49891..50295)
                     /gene="hofP"
                     /locus_tag="ACHK07_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO8100453.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPRWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50285..50725)
                     /gene="hofO"
                     /locus_tag="ACHK07_000055"
     CDS             complement(50285..50725)
                     /gene="hofO"
                     /locus_tag="ACHK07_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO8100454.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRVSHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50709..51248)
                     /gene="hofN"
                     /locus_tag="ACHK07_000056"
     CDS             complement(50709..51248)
                     /gene="hofN"
                     /locus_tag="ACHK07_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO8100455.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51248..52027)
                     /gene="hofM"
                     /locus_tag="ACHK07_000057"
     CDS             complement(51248..52027)
                     /gene="hofM"
                     /locus_tag="ACHK07_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO8100456.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52147..54699
                     /gene="mrcA"
                     /locus_tag="ACHK07_000058"
     CDS             52147..54699
                     /gene="mrcA"
                     /locus_tag="ACHK07_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO8100457.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYYGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESVWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54860..55420)
                     /gene="nudE"
                     /locus_tag="ACHK07_000059"
     CDS             complement(54860..55420)
                     /gene="nudE"
                     /locus_tag="ACHK07_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO8100458.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55740..57875
                     /gene="igaA"
                     /locus_tag="ACHK07_000060"
     CDS             55740..57875
                     /gene="igaA"
                     /locus_tag="ACHK07_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO8100459.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLIDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57940..58608
                     /gene="yrfG"
                     /locus_tag="ACHK07_000061"
     CDS             57940..58608
                     /gene="yrfG"
                     /locus_tag="ACHK07_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO8100460.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEFMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58619..59020
                     /gene="hslR"
                     /locus_tag="ACHK07_000062"
     CDS             58619..59020
                     /gene="hslR"
                     /locus_tag="ACHK07_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO8100461.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            59045..59923
                     /gene="hslO"
                     /locus_tag="ACHK07_000063"
     CDS             59045..59923
                     /gene="hslO"
                     /locus_tag="ACHK07_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO8100462.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59986..61710)
                     /gene="yhgE"
                     /locus_tag="ACHK07_000064"
     CDS             complement(59986..61710)
                     /gene="yhgE"
                     /locus_tag="ACHK07_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO8100463.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYCYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            62089..63711
                     /gene="pckA"
                     /locus_tag="ACHK07_000065"
     CDS             62089..63711
                     /gene="pckA"
                     /locus_tag="ACHK07_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO8100464.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63787..65139)
                     /gene="envZ"
                     /locus_tag="ACHK07_000066"
     CDS             complement(63787..65139)
                     /gene="envZ"
                     /locus_tag="ACHK07_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO8100465.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65136..65855)
                     /gene="ompR"
                     /locus_tag="ACHK07_000067"
     CDS             complement(65136..65855)
                     /gene="ompR"
                     /locus_tag="ACHK07_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO8100466.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66083..66559
                     /gene="greB"
                     /locus_tag="ACHK07_000068"
     CDS             66083..66559
                     /gene="greB"
                     /locus_tag="ACHK07_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO8100467.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66656..68977
                     /gene="yhgF"
                     /locus_tag="ACHK07_000069"
     CDS             66656..68977
                     /gene="yhgF"
                     /locus_tag="ACHK07_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO8100468.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69415..69642
                     /gene="feoA"
                     /locus_tag="ACHK07_000070"
     CDS             69415..69642
                     /gene="feoA"
                     /locus_tag="ACHK07_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO8100469.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69659..71980
                     /gene="feoB"
                     /locus_tag="ACHK07_000071"
     CDS             69659..71980
                     /gene="feoB"
                     /locus_tag="ACHK07_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO8100470.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71980..72216
                     /gene="feoC"
                     /locus_tag="ACHK07_000072"
     CDS             71980..72216
                     /gene="feoC"
                     /locus_tag="ACHK07_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO8100471.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISKTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72419..73297
                     /gene="rpnA"
                     /locus_tag="ACHK07_000073"
     CDS             72419..73297
                     /gene="rpnA"
                     /locus_tag="ACHK07_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO8100472.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(73324..74094)
                     /gene="bioH"
                     /locus_tag="ACHK07_000074"
     CDS             complement(73324..74094)
                     /gene="bioH"
                     /locus_tag="ACHK07_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO8100473.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSAREEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            74132..74815
                     /gene="gntX"
                     /locus_tag="ACHK07_000075"
     CDS             74132..74815
                     /gene="gntX"
                     /locus_tag="ACHK07_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO8100474.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74874..75449
                     /gene="nfuA"
                     /locus_tag="ACHK07_000076"
     CDS             74874..75449
                     /gene="nfuA"
                     /locus_tag="ACHK07_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO8100475.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75810..77126
                     /gene="gntT"
                     /locus_tag="ACHK07_000077"
     CDS             75810..77126
                     /gene="gntT"
                     /locus_tag="ACHK07_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8100476.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77171..79255)
                     /gene="malQ"
                     /locus_tag="ACHK07_000078"
     CDS             complement(77171..79255)
                     /gene="malQ"
                     /locus_tag="ACHK07_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO8100477.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79265..81658)
                     /gene="malP"
                     /locus_tag="ACHK07_000079"
     CDS             complement(79265..81658)
                     /gene="malP"
                     /locus_tag="ACHK07_000079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO8100478.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82270..84975
                     /gene="malT"
                     /locus_tag="ACHK07_000080"
     CDS             82270..84975
                     /gene="malT"
                     /locus_tag="ACHK07_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO8100479.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNLQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(85018..86034)
                     /gene="rtcA"
                     /locus_tag="ACHK07_000081"
     CDS             complement(85018..86034)
                     /gene="rtcA"
                     /locus_tag="ACHK07_000081"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO8100480.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFPL
                     HEQYIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(86038..87264)
                     /gene="rtcB"
                     /locus_tag="ACHK07_000082"
     CDS             complement(86038..87264)
                     /gene="rtcB"
                     /locus_tag="ACHK07_000082"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO8100481.1"
                     /translation="MNYELLTTENASVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87451..89049
                     /gene="rtcR"
                     /locus_tag="ACHK07_000083"
     CDS             87451..89049
                     /gene="rtcR"
                     /locus_tag="ACHK07_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO8100482.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIVSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(89031..89789)
                     /locus_tag="ACHK07_000084"
     CDS             complement(89031..89789)
                     /locus_tag="ACHK07_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO8100483.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89806..90636)
                     /gene="glpG"
                     /locus_tag="ACHK07_000085"
     CDS             complement(89806..90636)
                     /gene="glpG"
                     /locus_tag="ACHK07_000085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO8100484.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90681..91007)
                     /gene="glpE"
                     /locus_tag="ACHK07_000086"
     CDS             complement(90681..91007)
                     /gene="glpE"
                     /locus_tag="ACHK07_000086"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO8100485.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            91197..92702
                     /gene="glpD"
                     /locus_tag="ACHK07_000087"
     CDS             91197..92702
                     /gene="glpD"
                     /locus_tag="ACHK07_000087"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO8100486.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHFARTYGSNSELLLGNAGAVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92917..93507)
                     /locus_tag="ACHK07_000088"
     CDS             complement(92917..93507)
                     /locus_tag="ACHK07_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100487.1"
                     /translation="MNKIRLYIYPLYLGFVIVPLLVWPTVVVVGITLSFLMEMLFSNS
                     FMHRRISLLELQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPVYLWL
                     ILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLGFALG
                     YYCQWRSKNRCQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(93522..94286)
                     /locus_tag="ACHK07_000089"
     CDS             complement(93522..94286)
                     /locus_tag="ACHK07_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100488.1"
                     /translation="MQCSPAMQALIEQIYHIFRRYPVPSRFHVCCPYCFSEEQQQALR
                     GVSLRAIPFPLLHAWGTSIGPTPQNDDEIRYLLPRLFEFIAQRQFPGIYEGCCLQRIA
                     EADNQNWRTDELQIISDFAYLYMQDWVSAKEVVELEIILEMFYRGGIKIEPLLDAIMS
                     ISGYWPTASMACLLWRNSKEYVENSDFEQSDDNKTITTQINTWARNNHPLLKERARQA
                     IENPLKQPEPMTEYQVWEDDWMIDECLCAMYDASPE"
     gene            complement(94389..95897)
                     /locus_tag="ACHK07_000090"
     CDS             complement(94389..95897)
                     /locus_tag="ACHK07_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8100489.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDIWAW
                     QPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSMTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96022..98469)
                     /gene="glgP"
                     /locus_tag="ACHK07_000091"
     CDS             complement(96022..98469)
                     /gene="glgP"
                     /locus_tag="ACHK07_000091"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO8100490.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(98488..99921)
                     /gene="glgA"
                     /locus_tag="ACHK07_000092"
     CDS             complement(98488..99921)
                     /gene="glgA"
                     /locus_tag="ACHK07_000092"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO8100491.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(99921..101216)
                     /gene="glgC"
                     /locus_tag="ACHK07_000093"
     CDS             complement(99921..101216)
                     /gene="glgC"
                     /locus_tag="ACHK07_000093"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO8100492.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101234..103207)
                     /gene="glgX"
                     /locus_tag="ACHK07_000094"
     CDS             complement(101234..103207)
                     /gene="glgX"
                     /locus_tag="ACHK07_000094"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO8100493.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDTNGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103204..105390)
                     /gene="glgB"
                     /locus_tag="ACHK07_000095"
     CDS             complement(103204..105390)
                     /gene="glgB"
                     /locus_tag="ACHK07_000095"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO8100494.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(105663..106766)
                     /gene="asd"
                     /locus_tag="ACHK07_000096"
     CDS             complement(105663..106766)
                     /gene="asd"
                     /locus_tag="ACHK07_000096"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8100495.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            106958..107551
                     /gene="yhgN"
                     /locus_tag="ACHK07_000097"
     CDS             106958..107551
                     /gene="yhgN"
                     /locus_tag="ACHK07_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO8100496.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(107597..108892)
                     /locus_tag="ACHK07_000098"
     CDS             complement(107597..108892)
                     /locus_tag="ACHK07_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO8100497.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(108799..110895)
                     /locus_tag="ACHK07_000099"
     CDS             complement(108799..110895)
                     /locus_tag="ACHK07_000099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO8100498.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(111035..112375)
                     /gene="gntU"
                     /locus_tag="ACHK07_000100"
     CDS             complement(111035..112375)
                     /gene="gntU"
                     /locus_tag="ACHK07_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8100499.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(112379..112906)
                     /gene="gntK"
                     /locus_tag="ACHK07_000101"
     CDS             complement(112379..112906)
                     /gene="gntK"
                     /locus_tag="ACHK07_000101"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO8100500.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(113045..114040)
                     /gene="gntR"
                     /locus_tag="ACHK07_000102"
     CDS             complement(113045..114040)
                     /gene="gntR"
                     /locus_tag="ACHK07_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO8100501.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(114264..114959)
                     /gene="yhhW"
                     /locus_tag="ACHK07_000103"
     CDS             complement(114264..114959)
                     /gene="yhhW"
                     /locus_tag="ACHK07_000103"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO8100502.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(115082..116119)
                     /gene="yhhX"
                     /locus_tag="ACHK07_000104"
     CDS             complement(115082..116119)
                     /gene="yhhX"
                     /locus_tag="ACHK07_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8100503.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            116454..116942
                     /gene="yhhY"
                     /locus_tag="ACHK07_000105"
     CDS             116454..116942
                     /gene="yhhY"
                     /locus_tag="ACHK07_000105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8100504.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            117153..>117892
                     /locus_tag="ACHK07_000106"
     CDS             117153..>117892
                     /locus_tag="ACHK07_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO8100505.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IENTINDITHRFNSSYDINEKTRLINELKMESETARKEYQLFRQGNYDKYITTDIFEE
                     HGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLS
                     PLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNR
                     LIRPSLGNRPGTGRLFHYTSVDFKNKFS"
BASE COUNT        28623 a        31969 c        29466 g        27834 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcacgagcag caccaccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta
      541 gttttaccgt ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca
      601 aatttttctt tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc
      661 acgggacttt ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc
      721 aacgttactc ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt
      781 ggtcagagaa cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg
      841 gatcttaacg ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa
      901 gtcaccgata acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg
      961 ctcaagcaga actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag
     1021 tttaaacgcc agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc
     1081 catgtctact accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt
     1141 atcaacggcc gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc
     1201 gtagcctttc gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata
     1261 ctgaccacga ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg
     1321 gatagtttca cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg
     1381 cttcatacgg tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg
     1441 gttagattct tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag
     1501 agccagaccc attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac
     1561 cggctcaggg aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc
     1621 accagtggtt acgtctttca gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt
     1681 gatctcttca cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc
     1741 tttcacggag ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa
     1801 ggtcaggtta ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc
     1861 gtcatcactt gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat
     1921 cgcaggtacg tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac
     1981 acctttgttc ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg
     2041 ctgacgcaga gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc
     2101 catcagctct tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc
     2161 cagttcaacc atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc
     2221 gttccagttg atagctttca ttttcaccag gtcaacaaca cctgtgaaat gttcttcagc
     2281 accaatcgcc agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac
     2341 aactttcagg aaattcgcac ccatgcggtc cattttgtta acgaacgcaa tgcgcggaac
     2401 tttatatttg tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc
     2461 gcagtaaacc attaccgcac catcgagaac acgcatggaa cgttctactt cgattgtgaa
     2521 gtcaacgtgc cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat
     2581 accagaccag aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg
     2641 ctccatccag tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc
     2701 ggtgtagaac agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat
     2761 accgatgtta cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct
     2821 tttaggcgtt caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg
     2881 actaaaactc cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat
     2941 acggtgaacg tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag
     3001 ttcgttcgcc aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat
     3061 ccaacgcatt gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt
     3121 agaaccacca acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac
     3181 ttcgaatgct tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta
     3241 tacgatagat tcagcagtag attttttacc atctaccatc aggatattta caaatttagc
     3301 cagcagttct gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg
     3361 acgtggcatg gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt
     3421 ttgacattta agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg
     3481 cttcacgcca tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc
     3541 accacgtacg gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag
     3601 gatcacggag tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa
     3661 accgttagtc agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt
     3721 ggtagtatat acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac
     3781 gttgcttttc gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat
     3841 taaatagctc ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa
     3901 tatgaggacg ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc
     3961 cgccatcacc aggccatctg gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag
     4021 tcaatgggaa tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg
     4081 ttcagggcat agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca
     4141 acggcggcag ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc
     4201 agcagcgcag caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc
     4261 tcaaaacctc aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag
     4321 cggcgtggct tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc
     4381 cgcacaaacc cagcactgtt caatgtcgta cagacccaac aatttaaaag tggcaatgta
     4441 atcacgcgcc agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc
     4501 aataaagaag acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc
     4561 ttcccgcccg acagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat
     4621 cagaactgta ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt
     4681 aaggtaaatc cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccgtttca
     4741 tcaacaacgc cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc
     4801 gcattcaact gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag
     4861 tggttagcgt tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct
     4921 gctatcagcg cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc
     4981 ccggtcacca cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga
     5041 actggcggat gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt
     5101 tgatcgcatc tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat
     5161 ttttggcgtt attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg
     5221 tttgggtaac cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg
     5281 tttctggcgg cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga
     5341 tgcacagcag accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca
     5401 ttaatacgcc gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg
     5461 tcatatcgtg cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg
     5521 tatgttcacc gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa
     5581 cgatttcaca gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt
     5641 tcagaatatc aaaatcggtc tggtcgaaag gacgttgatc cagtaaatcc aactcactgg
     5701 tttcgttggt taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg
     5761 ttaacttttc agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt
     5821 ccgctttcca gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg
     5881 gcggttctta atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt
     5941 gcatcagcct tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa
     6001 ttcggtggga tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt
     6061 ttgatcttac cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg
     6121 tccagacgga aagaaagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg
     6181 atcagcgtac ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta
     6241 ccggcttcta ctacctgata aaccagacca gttgaagagg ttttcacacc tttctctttg
     6301 gcaaatttct cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc
     6361 ttcgcctgag cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct
     6421 tggtcggaga gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct
     6481 ttatccagtt tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc
     6541 agcgaggcac ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg
     6601 tcagcagttg tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca
     6661 acggccattg tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg
     6721 atcggggcat ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga
     6781 acaaatctac atacagacac gccctattat catctatttt cagactcttt ttgtttaaat
     6841 tagtttcgat gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg
     6901 gcgaccattc gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca
     6961 gagctggaga gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg
     7021 accgctcatg aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag
     7081 ttaaaagcca gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat
     7141 tattgaggcg taaaaaaagc ggggattccc cgcttttttg tcactttttc ggtattagtg
     7201 gcaaccgcaa ccgccgttac ctttaccgcc acagcagcct tcgccaccgt gctcatgacc
     7261 gtgatcatgg ccatgaccac cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg
     7321 cgcaccgtga acgtggccat gagccagctc ttcttcagtc gcttcgcgaa tcgccacaac
     7381 ttcaacgttg aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg
     7441 atcgtcttca accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa
     7501 acgcatacct acctgcagtt catcaacgcc cataaatacg tctttaggca cacgttgcac
     7561 caggttttcg tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc
     7621 gccaacttca tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg
     7681 caggtagtcc agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg
     7741 tacctgatag gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca
     7801 tgggaagaat agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa
     7861 aaaaactcgg cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc
     7921 gaacgtgatc gcgagcttct ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt
     7981 caacaatatc aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacagaccg
     8041 ggcattttgc gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt
     8101 catcccagcc gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca
     8161 gttcgcgtcg ggcttccctg gcgcgagaaa tttgtacggt atcggtatgc attgggatga
     8221 gctctcgcag catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat
     8281 gcggatgcat accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg
     8341 tttcacggga aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc
     8401 gcgctcgcgc aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca
     8461 tggtgtcttc cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag
     8521 aacgtaaaag atctacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttgcgca
     8581 tcaggttaac ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca
     8641 acaaacgacc aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat
     8701 catcgttgac ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc
     8761 atttatccac cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca
     8821 gcaacagcgc catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga
     8881 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8941 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     9001 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc
     9061 cgacagagat aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca
     9121 gttcatggcg atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca
     9181 tcgacagccc caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg
     9241 cggtgaacac ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat
     9301 agcgcccgcc aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt
     9361 cgaaatgttc gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg
     9421 ccagatcctg aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc
     9481 ctttctcacg catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac
     9541 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9601 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct
     9661 tggagggatt caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa
     9721 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9781 ctgccagcaa atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag
     9841 ccaccgccgc gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg
     9901 gcctcctgga gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag
     9961 ctcctgtgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg
    10021 gcacatgccc gccgccagtt caaaggggcg caggacatcg ctcatcgggt agcgattcaa
    10081 cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt
    10141 tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag
    10201 ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac
    10261 ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg
    10321 cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg
    10381 gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg
    10441 agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg
    10501 cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct
    10561 gaacatacgg caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt
    10621 cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa
    10681 aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg
    10741 cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc
    10801 gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga
    10861 agcgcagcta cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg
    10921 caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg
    10981 cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg
    11041 tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga
    11101 accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta
    11161 tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa
    11221 aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga
    11281 agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg
    11341 cgtagcgcat ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca
    11401 ggcccagagc cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga
    11461 caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat
    11521 ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct
    11581 ggtccccggt tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat
    11641 caaactgtta gccggtgaac ttgcgccagt cagtggtgaa ataggcctgg caaaagggat
    11701 caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat
    11761 tcaacatctg gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg
    11821 cggctttggt ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga
    11881 aaaagcccgc ctggtgctgg cattaattgt ctggcagcgg ccgaatctgc tgctgctcga
    11941 cgaaccgact aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga
    12001 gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga
    12061 cgacctctac ctggttcacg atcgtaaagt cgagccgttc gacggcgatc tggaagatta
    12121 tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgacg cgccaaaaga
    12181 gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agttgcgtgc
    12241 gcaaacccag ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa
    12301 cgcgcaactg gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg
    12361 taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga
    12421 gtgcgaaatg gcatggctgg aagcccagga gcagcttgag cagatgttgc tggaaggcca
    12481 aagcaactga tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa
    12541 ttcaccccta tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt
    12601 cgtcgcaagg tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt
    12661 gtcgatctcg cgtggagtga agccccagca caggcgcggc ataaaccgcg tttagtggta
    12721 tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg
    12781 caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac
    12841 cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg
    12901 ctgcaacgcg aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat
    12961 atgctggcct gtttgctggc aaaagaaggc aatgatctcc ctgttgatgc ggcggtgatt
    13021 gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc
    13081 gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc
    13141 taccccggaa cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa
    13201 tttgacgatc tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt
    13261 cagtgtagcg ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc
    13321 aaagacgatc cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag
    13381 gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg tacattactt
    13441 catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg
    13501 aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa
    13561 gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag
    13621 tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc
    13681 acgaaacggt caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg
    13741 gacgagataa taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat
    13801 tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat
    13861 attcgcgcag ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac
    13921 ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta
    13981 cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca
    14041 gagcggcaaa gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa
    14101 tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt
    14161 ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga
    14221 cttactggtc ggcgtggtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga
    14281 caccagcgag cgcgggcact cacgagaagc agtgatggac tcagtagtgc gttcaatgga
    14341 agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt
    14401 tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag
    14461 ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat
    14521 gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtacctggcg gcaaaatggg
    14581 gctggcaatg gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat
    14641 cgagtaactt tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa
    14701 atcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg
    14761 ccactgaaca atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca
    14821 ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt
    14881 cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca
    14941 ggatagatac cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg
    15001 gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctgatgacca gaggcggatt
    15061 cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac
    15121 tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct
    15181 gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag
    15241 gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca
    15301 gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc
    15361 ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca
    15421 aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc
    15481 catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta
    15541 aaggctctgt ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc
    15601 tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg
    15661 catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc
    15721 aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc
    15781 tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg
    15841 cacagactct gcttaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc
    15901 aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg
    15961 gacgcattct gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg
    16021 tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt
    16081 gtttcccccg atgtggcgca gactgattta tcaccccgat attaactatg cactacgaca
    16141 aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg
    16201 tctgctcttc tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa
    16261 acgttttttc aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac
    16321 acagctgcta ctggcaaaag atgttcccct gccctttttg ctgaccggat taacgctggt
    16381 acttggcgtc accgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct
    16441 ggcagccatt tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat
    16501 ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg
    16561 cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga
    16621 agccaaatac agcctgctta cccagcacac cgcccctgaa aaagcgctgc cgccgctgct
    16681 ggtgcgccag caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct
    16741 ttccgcgcaa aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga
    16801 tttgcaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga
    16861 gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg
    16921 cgtgctggct gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca
    16981 aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg
    17041 ctattggcac ttcagccgca ttgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg
    17101 tgacttactg gccgataaac agcgacgaat gccgttactt ccggcgctga aaagttatct
    17161 gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc
    17221 cagcttgatg ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt
    17281 gctggtgacg caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg
    17341 aaccgtggtc gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta
    17401 caccctgacc ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta
    17461 cggctgggcg acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct
    17521 gaacggtgag cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc
    17581 tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc
    17641 ccatgacgct ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca
    17701 accaacgcca ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa
    17761 ctcattgaat caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa
    17821 actgtgggta acgcacagcc agtttattgt tgagcatatt aatgctatga ccacgctggc
    17881 acgggaacac cgggcattgc cacctgaact ggcgcaagaa tatttgcagt cttgtgaaat
    17941 cgccattcag cgttgtcagc agcggctgga gtatgacgaa ccggggagtt ctggcgatgc
    18001 caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca
    18061 gcatttacag cggattattg gtcatctgaa caccatgcac acaatttcgt cgatggcatg
    18121 gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaagacgta
    18181 atacaggcta aaccatcgga tgaggtgttc acgccgcatc cgacatcttt ttcctgatgc
    18241 gtcttattta cgccccaaca actttcgcca ccgcgtgggc gaaacgttgc atcccttcat
    18301 cgatatccgc atcttccacc accagcgacg gtgcaaaacg catcacatcc ggtccggcat
    18361 tcagcaccat tacgccagcc tctgcgcccg catacaggaa atcacgcgcc cgacctttgt
    18421 actgtggctt aagctccgcg ccaatcaaca gccccatacc gcgaatatcg ctaaacacat
    18481 cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc
    18541 cttccagcac ttcaggtgta ttgatgatat caaacgccgc ccccgctact gcacaggcca
    18601 gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg
    18661 ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa
    18721 tatccggcgt cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc
    18781 actgcacttc atcaaacacc aataatgcct gatattgatc gcacagctcg cgcaagccct
    18841 gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca
    18901 ccgcacaggt gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt
    18961 ggatgatgtc tgctggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg
    19021 aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga
    19081 acgggctatg acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt
    19141 ccgtgccaga gttcataaac accacacgtt cggcaaacgt tgcctcaatc agtttacgcc
    19201 caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct
    19261 gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc
    19321 cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt
    19381 ttaccggaat gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc
    19441 gtgtaattgc tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg
    19501 tgaaattata atcacaaaat atgcataaaa aatcattgaa tggcaatcag aaatcagcga
    19561 tgtaggaaat tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac
    19621 tgcacacctt ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc
    19681 caggccgtca cgtcaaagca cgcaggtaat gagtcaggtt ccaccaccag cgaatgatag
    19741 cgtgtcacag taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc
    19801 ggcgaggttt tgccgtgcat gacctttgca gcgcgcacaa ctttaccgcc aaatgcctgc
    19861 gccattgcct gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga
    19921 ataacgtcaa gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaatt
    19981 ttttgtggtt taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc
    20041 acatccgccc ccagttcaca aaaatactgg tagaggttcc aggtaaaaga atcgtagtta
    20101 tctataagca ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc
    20161 cccggcttcg cttactactt tgctaaatat catctgcagt gcggtgagat cccccattgc
    20221 cccactctga tttgcctgat tccaggcctc tttttcaata ccctgccagc tcagttgata
    20281 tccggcatga atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg
    20341 gaagggatgc agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc
    20401 tttctccagg ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc
    20461 tttttcgata taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg
    20521 cagttgccct gcccagtcga aaatatcctg atacagctgg cgatggatag tgcgcaaatg
    20581 cggtaaaccg cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc
    20641 gtaagcggcc tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc
    20701 aaggcctgga taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc
    20761 tcagctcttc aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga
    20821 cgccttcaag acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg
    20881 acttttgctt atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac
    20941 aagtataagc ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg
    21001 cgcgagaaat cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac
    21061 attctggtac ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat
    21121 gcctttcacc actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa
    21181 ggcgttatcg gcaacgttga taaagaactg gctggtggcg ctgtctttgt cggcggtacg
    21241 cgccatcgcg atggtaccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg
    21301 cgggtttggt tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc
    21361 aggaatgacg cggtgaaagg tagtattgtt ataaaaaccg ctgttcacat aatcgacaaa
    21421 gttctgcaca gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga
    21481 ggttgtcaac aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga
    21541 aagagcgaaa acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca
    21601 aacagagcag aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc
    21661 cctttaccta attttacgta tctgaaatat atgtaacctg acgatcactt tttattccgt
    21721 gatcaaaatc acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaaacag
    21781 atcacaaaat cacctaaaat cgcccgctcg cggcatatcc cgctatgcca ttttattttt
    21841 gaacgcaggc caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctc
    21901 tcctacgcac tgactggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc
    21961 gattatttca atctgcctgt ttccagtatg agtaacacct tcaccttcct caacgccggc
    22021 attttaatct ctatattcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag
    22081 ttacgttttg gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc
    22141 ctggcgctgt tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg
    22201 attggtacat tcctggtaac acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta
    22261 tttaccgact ccttcttcag tatggctggg atgattttcc caatgatcgc cgcgtttcta
    22321 ctggcgcgca gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgtcgct
    22381 atttttattc tgactttcgg ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg
    22441 gatgctccgg tagcgaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg
    22501 ctgtgctaca tcctcggtca gttaggtttt atctcctggg tgcctgagta tgccaaaggc
    22561 ctgggcatga gcctgaacga cgcgggcacg ctggtgagta acttctggat gtcatacatg
    22621 gtcggcatgt gggcgttcag ctttattctt cgcttctttg atttgcaacg cattctgacc
    22681 gtactggctg gtctggctgc gattctgatg tacgtcttta acaccggaac accggcacat
    22741 atggcgtggt caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc
    22801 accctgggtt cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc
    22861 tgcgggacca tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagc
    22921 ggtccgcagg cggcactgct tacagcaaac ggtctgtacg ctgtcgtctt tgtaatgtgc
    22981 ttcctgttag gttttgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg
    23041 ctcatgccgg acggcactat cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat
    23101 ctatttctat aaacccgttc attttgtcca ttttttgcac aaacatgaaa tgtcagacaa
    23161 ttccgtgact taagaaaatt tatacaaatc agcaatatac cccttaagga gtatataaag
    23221 gtgaatttga tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga
    23281 aaagaaatcg aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc
    23341 ggccatcgct ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc
    23401 gttttctgtg aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct
    23461 caccacaccg ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc
    23521 aaagttctga tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc
    23581 agcgccgggc gcaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg
    23641 atcccgccaa tcaaaggttc tgatactcag gactgctttg tctatcgtac tattgaagac
    23701 ctcaacgcca ttgaatcctg cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc
    23761 ctgttaggtc tggaagccgc tggcgcgctg aaaaacttag gtattgaaac ccacgttatc
    23821 gaatttgccc ctatgctgat ggcggaacag cttgatcaga tgggtggcga gcagctgcgt
    23881 cgcaaaatcg aaagtatggg cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg
    23941 caggaaggtg tcgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc
    24001 gactttatcg tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt
    24061 ctggacgttg ctccgcgtgg gggtattgtt attaatgatt cctgccagac ttccgatccg
    24121 gatatctacg ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca
    24181 ccaggctaca aaatggcgca ggtcgccgtt gaccatattc tcggtagcga aaacgccttt
    24241 gaaggtgctg accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt
    24301 gatgcgcacg gtcgcacacc aggcgcacgt agctacgttt acctcgacga aagcaaagag
    24361 atctacaaac gccttattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg
    24421 ggcgatacca gcgactacgg caacctgctg caactggtgc tgaatgctat cgagctgccg
    24481 gaaaacccgg attctctgat tctgccggcg cactcgggta gcggtaagcc gtctatcggt
    24541 gttgataaac tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat
    24601 ctgattgctg ccatcaacaa aggctgtcac accgttgcgg cgctgaaagc cgaaaccaaa
    24661 gcgggtactg gctgcggtgg ctgtatcccg ctggtcactc aggtactgaa cgcggaactg
    24721 gcgaaacagg gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag
    24781 gaactgttcc atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa
    24841 cacggcaaag gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc
    24901 tgctggaacg aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac
    24961 ttcctcgcta acatccagaa agacggcacc tactcggtga tcccgcgctc tccgggcggt
    25021 gaaatcaccc cggaagggct gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac
    25081 accaaaatca ccggctccca gcgcctggcg atgtttggcg cacagaaaga cgatctgccg
    25141 gaaatctggc gtcagctgat tgaagccggt ttcgaaaccg gtcatgccta tgcgaaagca
    25201 ctgcgtatgg cgaaaacctg cgtgggtagc acctggtgcc gctacggcgt gggcgacagc
    25261 gtgggcctcg gcgtggaact ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg
    25321 aagttcggtg tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt
    25381 attatcgcca ctgaaaaagg ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg
    25441 cgtcatgcgg atctgctggc ggcggatatc gatcgcgaaa cgctgatcaa atatctcgac
    25501 cgcttcatga tgttctacat ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta
    25561 gaaaacctcg aaggcggcat cgattacctg aaagcagtga tcattgacga caaactgggg
    25621 ctgaacgcac atctggaaga agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg
    25681 actgaaacgg tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac
    25741 aaacgtgacc cgaacgtaca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg
    25801 tatgaacgta tcccggtaac tctggtggag gacaacgcat gagccagtgg aaagacatct
    25861 gcaaaatcga tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg
    25921 tcgcgatttt ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt
    25981 tcttcgagtc cagcgtgcta tcacgcggac tgattgcgga acaccagggc gagctgtggg
    26041 tcgccagccc gctgaaaaaa cagcgttttc gcttaagcga cggcttgtgc atggaagacg
    26101 aacagttttc cgtcaaacat tacgaagcgc gagtgaaaga cggcgtggtg cagctgcgcg
    26161 gttaatgttt taacgggagg cgcaatgcct cccctttttg catggtcctg taataatctt
    26221 cggtatattg caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa
    26281 ggataatcaa atgtttacag acactattaa taagtgtgcg gctaacgctg cgcgcattgc
    26341 acgcctgtcg gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta
    26401 tgtgggtctt gggatcatcc tgatttttac gctcggtaat ttgctcgatc catccgtacg
    26461 ccctctggtg atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc
    26521 tgaactgttc accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag
    26581 ccacgggcaa atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt
    26641 cttcgttgcc atgctttata gctggggggg cggtagcctg ctgccggtag ataccagcat
    26701 cgttcactcc gtcgcgctgg ctaaaaccac tgcaccggca atggtactgt tcttcaaagg
    26761 tgcattgtgt aactggctgg tttgtctggc aatctggatg gcgctgcgca ctgaaggggc
    26821 ggcgaaattt atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca
    26881 ctccatcgct aacatgacgc tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta
    26941 cacgctggcg ggtattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcagg
    27001 tgccgtattc atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc
    27061 ggacaaattt aatcaaactg aaacggctgc cggttaatta ctaaggggtt tttacgtgga
    27121 tcatttgcct atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga
    27181 tgtcgcggaa cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc
    27241 attagcgttt attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga
    27301 agggccattt gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga
    27361 tgacgcgctt aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt
    27421 ggtcgatgcg ccgaaagctg ccagcttcat tatgccgtcg attattgacc gctcaccgct
    27481 gatggtggcg gtttcctctg gcggcacctc tccggttctg gcacgcctgt tgcgcgaaaa
    27541 acttgaatca ctgctgccgt tacatctggg ccaggtagcg aaatacgccg ggcaattacg
    27601 cggtcgagtg aaacaacagt tcgccacgat gggtgagcgt cgccgtttct gggagaaatt
    27661 gtttgttaac gaccgcctgg cgcagtcgct ggcaaacaac gatcagaaag ccattactga
    27721 aacgaccgaa cagttaatca atgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg
    27781 tgcaggtccg ggcgatgccg ggctgctgac actgaaagga ctgcaacaaa ttcagcaggc
    27841 agatgtggtg gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga
    27901 tgctgatcgc gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaagagat
    27961 taaccagatc ctgctgcggg aagcgcaaaa aggcaaacgc gtggtgcgtc tgaaaggtgg
    28021 cgatccgttt atttttggcc gtggtggcga agagctggaa acactgtgca acgcgggtat
    28081 tccgttctcg gtggttccgg gtattaccgc agcttctggt tgctctgcct attcaggtat
    28141 tccgctcacg catcgcgatt atgcccagag cgtacgctta attaccggac acttaaaaac
    28201 cggtggcgag ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttttatat
    28261 ggggctgaat caggctgcga ctattcagca aaagctgatt gaacacggta tgcctggcga
    28321 aatgccggta gcaattgtcg aaaacggaac agcagtcacg cagcgcgtga ttgacggtac
    28381 gctcacacaa ctgggcgaac tggcgcagca aatgaacagt ccatcgctaa ttattattgg
    28441 tcgggttgtt ggcctgcgcg ataaactgaa ctggttctcc aaccattaat ttaatcaggg
    28501 ccagagaatt ctggcctttt tcgtttacaa ctctctttat atattcagca gataaaaaca
    28561 taacccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28621 aattaagtca ttcactcact gacgccgatt aaatattcct gtatcgttct taatgagcag
    28681 tatacttata ttcatataat caatgaatat taatcaatat ataatacata gggaatgtaa
    28741 atgaacaaat ttattaaagt tgcactggta ggtgcggtac tggctacgtt aactgcatgt
    28801 actggtcata ttgaaaaccg tgataagaac tgctcttacg actacctgct gcacccggca
    28861 atttctattt ctaaaatcat tggcggttgc ggtcctactg cacagtaagt ctctgacaga
    28921 aatcggctaa caccggttta attgcccgac gccctctgga ttttccgagg gcgtatttgt
    28981 ttctatatcc ttcttgctcc ccacccgtaa aaatattttc actttaattt caattggata
    29041 accacattca tcagccacag cgcgatcctg tgcgaaatta tgtgatcttc ctccacatta
    29101 cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc
    29161 aaactctccc ctacaacgtg ttggacaaga aaaaggtatc gctatgggaa gccaggaact
    29221 ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc
    29281 cggtattttt gtatctgtgg gtgaagtggc aaaagcggcg ggcacgccgt ggctgacggt
    29341 gctcgcattt gtcatcggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact
    29401 atccaccgct tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg
    29461 accgctggct ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc
    29521 gattatggcg ctggcgatag tcagcaatct tggcttttta acgcctatcg atccgttgct
    29581 cggtaaattt atcgccgccg gattaattat cgcctttatg ctgctgcacc tgcgctccgt
    29641 tgaaggcggc gcagcatttc agacgctaat taccatcgcc aaaattatcc cgttcactat
    29701 cgtcattggc cttgggatct tctggtttaa agcggagaat tttgccgccc ctaccaccac
    29761 tgcgattggc gcaacgggca gctttatggc gctactggcg gggatctccg ccaccagttg
    29821 gtcgtacacc ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggtaa
    29881 aactatgcca cgagcgctaa ttggttcctg tctgctggtt ctggtgctct acaccctgct
    29941 ggcgttggtg atttccgggc tgatgccctt cgacaaactc gccaactctg aaacgccgat
    30001 ttccgacgca ctgacctgga tcccggcact cggcagcacc gccgggatct tcgttgccgt
    30061 cacggcgatg atcgtcattc tcggctcgct ttccagttgc gtgatgtacc agccgcggct
    30121 ggaatatgcg atggcaaaag acaatctgtt cttcaaatgc ttcggccatg tgcatccgaa
    30181 atacaacacg ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt
    30241 cgtttcagat ctcaccagcc tgctgggcta tttcaccctg gtaatgtgtt tcaaaaatac
    30301 cctcaccttc ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg
    30361 tactccggct ttcgggctga tgaccaccct cgccattgca tcaagcctca ttctggttgc
    30421 ctcaaccttt gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgccac
    30481 tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta
    30541 atgttatctg gagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc
    30601 accgaaaata tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac
    30661 accatcgtgg aagagatggt gaagcgcgac atcgatcgta tttatttcgt tgcctgcgga
    30721 tcgccactca acgccgcgca aacggcgaaa catctggcag atcgcttttc cgatcttcag
    30781 gtttacgcca tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt
    30841 tgcgcagtaa ttggcgtttc tgactacggt aaaaccgaag aggtaatcaa agcgctggag
    30901 ctgggccggg cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt
    30961 acctcggcgg cggaatttag cattgattat caggccgact gtatctggga aattcatctg
    31021 ctgctctgct acagcgtggt gctggagatg atcacccgcc tcgcgccgca cgcggaaatc
    31081 ggcaagatca aaaacgatct caagcagctg ccaaatgcgc tcggtcatct ggtacgcacc
    31141 tgggaagaga aaggtcgcca gctcggtgaa caggccagtc agtggccgat gatttacacg
    31201 gttgccgcag gcccgctgcg cccgctgggt tacaaagaag gcatcgtgac gctgatggaa
    31261 tttacctgga cgcacggctg tgtgattgag agcggtgagt tccgtcacgg cccgctggag
    31321 attgtcgaac cgggcgttcc gttcctgttt ctgctcggca atgacgaaag tcgtcacacc
    31381 accgaacgcg ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac
    31441 gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg
    31501 gagtggctct gctactacct gtctatttac aaagatcaca acccggatga acgccgctat
    31561 tacggtggtc tggtggaata ttaatccctc tcccggcccg gcaacgggcc ggatttacgc
    31621 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
    31681 acacgcattc cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg
    31741 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacagatca aggcgctggc
    31801 gcagacgtat cagatgccga ttatcggtta tacgccagaa accaacggct atccgtataa
    31861 catgatgctg ggcgatgaac atatgcgtcg cgaaagcctg gacatgatca agctggcgat
    31921 ggatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc acgcgggcta
    31981 tctcacgccg cctaacgtta tctgggggcg gctggcagag aacttaagcg aactgtgtga
    32041 gtacgcggaa aacatcggca tggatctgat cctcgaaccg ttaacgccgt atgaatcgaa
    32101 cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct
    32161 gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt
    32221 cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga
    32281 cacgcattac attcctggag aaggcaaaat gccgctgcgg gaactgatgc gcgatattat
    32341 tgatcggggc tatgaggggt actgcacagt ggaactggta acgatgtata tgaacgagcc
    32401 cagactttac gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagtg
    32461 atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa
    32521 gcgttttctg gcggtaatgc ggtgaacgtg gcggtgtact gcactcgcta cggtataaag
    32581 ccgggatgca ttacctgggt gggtgacgat gactacggca caaagctgaa gcaggatctc
    32641 gcccgcatgg gcgtcgatat cagccatgtc catacgaaac acggcgtaac cgcacaaact
    32701 caggtggaac tgcacgacaa cgatcgcgtt tttggcgact acaccgaagg cgtgatggcc
    32761 gactttgccc tgagcgaaga agattacgcc tggctggcgc agtatgacat tgtgcacgcg
    32821 gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc
    32881 gctttcgact tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc
    32941 gattttgcct ttgcctctgc accgcaagaa gacgaagcgc tgcgtctgaa gatgaaagcg
    33001 attgttgccc gtggcgcagg aacagtgatt gtcacgctgg gcgaaaacgg cagcattgcc
    33061 tgggatggcg cgcagttctg gcgtcaggct cctgaactgg tgacggttat cgacaccatg
    33121 ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta
    33181 ccgcaggcga tggcgcaggg aacggcgtgc gcggcgaaaa ccattcagta ccacggtgcc
    33241 tggtaggtat aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa
    33301 cgaccctata atccgagtaa ttcattcttt atttcagggt cgattatgtc agctacggac
    33361 cgctactctc atcaactcct ctacgctacc gtccgccagc ggctgctgga tgatatcgcg
    33421 cagggggttt accaggccgg gcaacagatc cctaccgaaa acgagctttg tacacaatat
    33481 aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg
    33541 atccgctggc agggcaaagg cacctttgta caaagccaga aggtcgaaaa cgccctgctt
    33601 actgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg
    33661 atcgagcagg aacgggtcag cgccgcgccg ttttgcgaaa agctgaacat ccctggcaac
    33721 agcgaagtgt tccatctttg ccgggtgatg tatctcgata aagagccgct gtttattgat
    33781 agttcctgga tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc
    33841 tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc
    33901 atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa
    33961 ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc
    34021 gaactcttct gccgcgccaa ccgcatcacc ttaacgattg ataaccaacg acattaaccg
    34081 taggccggat aagatgcgcc agcatcgcat ccggcgatgc cggcgcactg aattttacat
    34141 cccgcacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gttcagtgcg
    34201 gaatttagct tgattgccga acggtgctat ccagcattaa atactggcaa tactctcacg
    34261 caaaatccgc agcaccgtct cttcaccgct gcgattcggg ttaatgcgaa tcgcacaatg
    34321 ttcgagttgc ggattcgcct ggcggaacgt tccggaaagg cgataaaaga gcggcgggat
    34381 ttcatatttc gactctgcac cgaccgggta aggcaacgcg ccacgctttt gcgcttcttc
    34441 cagcaccctg gcggcaatcg gctgatgaaa ctcgacaatc aacaccttcg actgcgcatt
    34501 ggcaatgacg gcgcttttca cttccggcac tgcaccaccg ttgagcaaag ccagcaaccg
    34561 ttcagatacc cctgcctgca ccgcgtgcat cactggcgcg aacaccagcc cacgcaacac
    34621 ttccaacgca tgagcgccct ggatctggct gccgccggag taaagcgtgg cgcgaatccg
    34681 actaataaca tcggcatcgc caaccactgc accaacgccc tccggcccaa acagcttgaa
    34741 gcaggagaat gtcgagacat tcgcgccgca ttcacagccg attcgcgcca ccttcatcac
    34801 cgcatagttg tcatcggtta acgctggaat gccagccagg cgcaacgttg ccagcacatc
    34861 tgccagcaca tagctgtcct gcggctgctg gcgcgtatgc tgcaccagcg cggcatcagg
    34921 ttgttgctcg tcgacgacct gcttcagtgc cgacaggtca ttgaaatcag cagtaataag
    34981 cgtcagcccc atctgctcaa taataacccg tgtcgttggg taaacaggcg cgtcatgcac
    35041 cagaagacgc tgccccggtt tgagcaaagc cgccagcgcc gcacgaatcg cgccagtacc
    35101 cgccccctgt accagcgccg ccgctggtgc atgaaacgca tcagccagca cctgctccac
    35161 ccgctgggta atgcgcggtt gattcaaccc tggagttaac cctaaatcac cgccagtaag
    35221 aaactcgctg ccggggaaat ggcggcagat ggtgtccacc aacgcaaact gcttttgctg
    35281 cgcttcatta agcgtcaggc tttgcagagg aaacgtcttc ataggtgcat tccttaagcc
    35341 ggaataaaca ggccaaacca gtaaagaaca ttcagcagga tgccggtgat cattactgcc
    35401 acgaccggtg ccgccatttt ctgtaccgga cggcccaggg attcgttgag gaagtaaatg
    35461 gcaaccgcga tagagaatcc ggtataaccc gccatcttaa ttgctgcgaa aatcgaaccg
    35521 accagcagcg ccacttccat cagcatattc atggcgttac ggatgttatc cgacgcatta
    35581 cgcaccgacg ggtagcgtcc cagccatttg ccgatagaac gaagcagcaa gacttccgcc
    35641 gaaataacca ctgcgcctaa tactgccgca accatcggat tcggtgagag atagcccacc
    35701 gcataaacaa aggtaaagcc cgcaaccgca tacacgccag tggctaacgc ggtggtggca
    35761 atcaacggca caaaacccag cccgcgcata aattccgcca gtgccgcctg attaatcagc
    35821 gtttgtgatt gttccggcgt tacgcctgcg gagtacgctt tctccagagt gaagatcgac
    35881 acttcactgc cggcgaaaat cttcatgctg gcaacggcgg caatcagcgc ccctacgatg
    35941 gcgatatacg gtaagttttt gataatccgc gacgtgcgct cttcaaacac cgaaagcccg
    36001 caggcatcga tgtcattttc atcacgatga cgcaggtcgt gagttatcgc gatccccagc
    36061 agcatcacca tgccaataaa gatttcgatg gattcagggt taagatgtgg gaaatagcgc
    36121 acgactacca cacgggtcat cagaacaacc acggcggcga ccagactttg cttccagcca
    36181 aactggtaga aaatcgccac cagcgggaac agtgcaaaag cagaaaccac cggcgagctt
    36241 aattccccga ggctacctaa tacatccacc ggcagcgcgg tcagcagctg gtttactggc
    36301 aacaggcaag taaggatcaa cacgccccag atagcgccaa gaccaaacgc catcaggctg
    36361 tttatcgcca gtacgccgag gatatcggta ggaagaaaga gtaaccatgc gtttagcagc
    36421 ccggttttca gggtaaaaga gatccccacc gaggccacaa aaccaatgct cagaccaaaa
    36481 gcgatactcc ccgcctcgcg acggttcata tagccttcaa tcagttgcgg caggatcggg
    36541 cggatgccgt catgaaaaac agccgccgag cgatgcgcca gaagcgatgt catacccgtc
    36601 aggcacgcca ccacgataat ctgaatatac agatccatag tcgtgccttt tatttcagat
    36661 ggttaatcag catcggaatc gcgtgttcga cgtgctcaac ggaaaggcca aacgccactt
    36721 ttccttcagc gatcattttg gcgatatgct cgtctttcgc tttaatgcct ggtttggcaa
    36781 tggtgcaact tttgttatag ccaatcaccg cgatggcaat cgacaatgcc gcgcccgcac
    36841 cggtattaca cgcgccgatg taataatcca gttgcccgga tttcaccttc attgccgctt
    36901 ccatgtcgtt gtgaatgaaa acttcaaaac agccaggagc cgtcgcttca atagtttttt
    36961 taatctgctc acgctgtaag cctgcaacgc caatcttttt catgagtctg tccttattgg
    37021 aaaaattgag agggattttc acgtaacatc acatccacat cggcctgact gaatcctgac
    37081 tggcgcaatt gcggaataaa ggtggttagt aaatagtcat agccataacc accgttggct
    37141 tttaaatggg agcggcgcgt aatatccatc gacagcatga cgcggttcag caacccacgg
    37201 tcgcgtagcg catgaagcat cgcaatacgc ttttcgtccg ggtagtaact gttcttacca
    37261 atggtgtcga actgcacata cgcgccgaga tcgatcatct tcaaaatgtt gtcgaggttg
    37321 tctttcagat cgcagtgacc aacggtgacg cgcgaaagat caactccgtg ggcttgcaac
    37381 aaagccagtt gctccagccc catcgtgctg aacgacgtat gcgtggagat cgggcgtccg
    37441 gtctggttat gtgccagcgc ggcggcaata aataccttct cttccagcgg cgtaatcttc
    37501 ccttcgctgg agccgatctc cgcgatgatc ccggctttca gttccgtgcc atcgataccc
    37561 tgctcaattt catcgaccat ctcctgcgcc agttcctgca cgctgcgggt cgccacatgt
    37621 tccgggaaaa acgcgtcctg gtaataaccg gtacaggcca ccacgttgat ccccgtctcg
    37681 cgcattacat cgagcataaa ttgcgcattg cgccccatgt aacggttggt catctcaatc
    37741 acattacgca cgccccgggc catcaggtcg ttcatctcct ggcaaatgaa cgaatactga
    37801 tcaaggcggc agtccacgtt gtttttaaag ccggagagat caatatgcag atgctcatgg
    37861 gcgagggtgt aacccgtcgg atcaaaactc atagggtgtc tcctgcaaac cggagggagg
    37921 aaagaaaaga gcgaccattt tgtggcggtg gcgcacggaa aaattcacac acggtagccc
    37981 ccacatcaga aagcgtggtg cgcacaccga tctgcgtagt gaccagccct tgctgataaa
    38041 ccagcactgg caccacttca cgggtatggt ggctgtgacc aatggtcgga tcgttaccgt
    38101 gatcagccat gacaaccagg caatcatctg gctgcatcgc ctcaaccagg agagcaagat
    38161 tacggtcaac aacctgcaaa cgttcggcat aacgtgcgac gtcttccgcg tgaccagcga
    38221 ggtcggtttc ctgaatgttg gtgcaaataa acgctgtctg atgggtggta aattcgtcga
    38281 gagtgatatc cataatccgc tggctatcca ccagattttg ccagctcacg ccataaggat
    38341 tgttgacgat atctgccacc ttacccacca gcacggttgg cacgcctgct tcatacagtt
    38401 tttgtggcac ctgcactttt tcatcgacgc catagcccat atgcacgacc tggaaaccgt
    38461 tgtcataagc gccagaacgc ggcgcattaa taccaataaa gcgcccttct ttgctttctg
    38521 cggcatcgag aatacgctga ctgtcggtta acaggccgcc aaatgtaatg acccgaccga
    38581 cctgtacctg gtcacgcacg atacgaccaa ttttgattgc gtcgtcaaaa gagatcacag
    38641 agagattggc ggtaatgtta tagacttggc ctaaatccgc ctcgagatta tcgccaatcg
    38701 caaccgcctg attgacccac agaaattgca gttcatcgcc acggcgctcc acctgccaac
    38761 cggcggatac taatgcctgc tcaacacggt caatcacatc gcgaaagggc atccgcagcg
    38821 gcggtaacgg gcgcgtgcct aaaatttcct gatgccccat aaaggtatcg ccaccttcat
    38881 gttgcagctc tgccacgccc caggttgcgg agtctgacgg ctgcatatcg cctggcgcat
    38941 aacccaatgc gttgattagc cccagcttct ccagcgttgg tagctgcaaa tgcggcaact
    39001 ggctcaggat gtgaccacag gtattcgccc cggcatcctg cggacgcacc agcgtgacat
    39061 ctttcattgc ccctacgcca aagctatcaa tcactaacac cacaaatcgc gccatttacg
    39121 cctccagagg gttgccgaga ctgtcataac gaccaacgat tttcggttcg ccacggtgaa
    39181 taccggacac cagcaccaca tcgctacggg tgacaaaaat ctgcgtgcgg aaacagagca
    39241 ccactgcact gcttaccgga aactcgcctg ccagcgacag ggtgtagtcg atactgctgt
    39301 catccacagt tttgagattg gtttcagtaa tcttttgatt ttctggcgta aacaccagcg
    39361 catgttgcgc atgaccacga cggtagtaac cgccgccgta gcagtagctg tcgccacgga
    39421 aatgatggga gatttcactt aaccagagca tcgcgatacg ttcaggctga tcgccctgct
    39481 ggtttgccgg aatagtaccg gtcagcgcat gaccgggttc ggcatgagtc gcaccgtatt
    39541 ccgccagcaa tggcagcgaa gtgcagctgg tcgctgaagg cgcgttcagt tgctcaatcg
    39601 caataccaga tttcgccagt tgatcccgtg cctgtatcag cgtgtgaaga ttcggtgtcg
    39661 gcaaaacttt cccggcagcc tcatcccaaa gcaggcaagg gaaatgggtt agtccggcta
    39721 aatgcagccc cggcagtttc ttaatttcag caaccacctc gtgcagcgaa tgctgtacaa
    39781 aaccgctctc ctgccccgga taaagaaaat catcgtcgct ataaactttg agcagcacag
    39841 actggattcg cccggccttc accgccgccg cagaaacttc ccgcgctttg tcgagagtaa
    39901 acacggtgat gacgtcggtg ccctgttcaa cggcgtcagc aacctgatga caagggattt
    39961 gtaccagatg cccctgatgc gccaccggca aaccagcgcg gcgcatgact cgcgcctctt
    40021 tgtaatccac cgccacaatg ccgctgtagc ccaacgccag cagtttttcc gccagccatg
    40081 ggttacgacc gaattgtttg gtcatcagat acagttcaat gccgtaaagc cgcgccgtct
    40141 caatcagccg tttaccgttt tccagtacct gatccacgtc gatcacccag ctgtccgggg
    40201 cgatcttgcc ctgctgccac aaacttagtg cggcggaaat cagcgccggg ttctggcgtt
    40261 tcaatgcttc tacaaacatc ttgttgcctc gtttctgttc cttgaattaa atattcattt
    40321 ttttgaatat ttagattgta cgacgtggat ttgtcgggag cttcaaacct cttcgttagc
    40381 agccatccat aatccataaa ggttggccag caaatagcct tcttcacacg ggttaacttc
    40441 cagtgcgaat tccttcaaca acacctgatg caattgcaca acggcaggcc agtgagtgga
    40501 ttgcgccagc tctgccagca attcgtcatc cagcggctct atttcttcac cacggcgact
    40561 gcgcatcagt gcactcgcca tatgtgtcat cgccatcgtt ccttgctcac tgcgcaccgg
    40621 tagatggcac tctgtttcca gtacgttgac gacctgcatc atgcctttgc agatgtcctt
    40681 atcaataacg cctgcatcgc aaagcaggtt gagtctggtt tccatatcag atgcctttcg
    40741 tttaataact gggttcctgt tccccactca ccacccgttg ttgatgggca agcagggcgt
    40801 gtttatcaac aactgcacgc agcagttgtt gtattgggta atcatcgact cgcgtcagga
    40861 ccactgcttc ggtggcctgt aaaaatcgcg gatcgtctgt gagcggcgtc gcctctaatc
    40921 ccagcgtggt cagttcgttt tccgccacca cgttccagat aaccgcatca acatcgcctt
    40981 taacaatgcg ttgtaaactc tcgtgataag agagatcgac tcgttccaca tcactaccgc
    41041 caaaaaagac atcagtcatg attttctgat ccgccgaacg gttatccagc cccacgcgct
    41101 tcacgtttgc ggactcgcct ttacggcaaa tcaactggtg ctcgccaacg taggtgtgcg
    41161 gccccaactc cagcgcgagg cataaccctt tttgcgtgag ataactttcc gccgccagtc
    41221 gggaaaccac cgccatgtca tacacgccgt taagtaaaca ctccacgcga atatccgcgc
    41281 cgcgcatatg cgcatagtaa aaaggaatgc catcaaactg ggctttcaat ccgctcgcta
    41341 aaccttcgta caagcgggta tagggcaaag gcatcgcaca taccacgttg ttgatatcca
    41401 catgactcag caatgctttg ttatccatct cgaccagata actaccattg cgcccacggc
    41461 gttcaatccg tatcgctccg cttgattcca gcgttttcaa cgcggcctgc gtcagaccaa
    41521 cggatgaacg gcattcagtg gccagttcat ctatagtttt cagtcgatta ccgcactttt
    41581 cacccaacaa ataacgggcc agcgtcgtga taacgacgcc ttcttttttg ataaacgttc
    41641 gacgcataat atattttcaa taaaatgaat gtttatatct tcattaaaat gaagataagt
    41701 ggcaacggtg agatgtggaa acggcgaggc gcttcacatt ttttcggatt attgcaaatg
    41761 aggataaaaa aaccgggttt ccccggtttc agagtgatga cgaagtgatt tattcgacaa
    41821 tgcgagcaaa gggtaattcc cactggcgct tgcctgctcc cctcaccctc tccccaaagg
    41881 ggcgagggga ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg
    41941 tacaccgctt ttagcgtacg ggaagcgtgc gcgctggctt tttctgcgcc gtctttcatc
    42001 acctgttgca ggaaggcttc atcgttgcgg aaacggtggt aacgttcctg caattcagtc
    42061 agcataccgg aaacggcatc agccacttcg cctttcagat gaccatacat cttgccttcg
    42121 aactgttttt ccagttccgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    42181 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggaatca
    42241 gtgaccgcgc gtttgatttt cttcactacc gatttcggat cttccagcag gccaataacg
    42301 ttattgcggt tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg
    42361 cgcgcgccgg atttcggaat aaacggctcc ggcaccttaa agatctcgcc gtacagcgcg
    42421 ttgaaacgct gagcgatatc gcggctcagt tccaggtgct gtttctggtc ttcacccacc
    42481 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagtcaaac
    42541 agaccagcgt tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcgg
    42601 ctcagttcgc cgaagtaggt atagcagttc agtgcccaac ctaactgtgc atgttccggc
    42661 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    42721 gccagcgtat ccagcgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    42781 tgttggtcaa cgatacagta aatgcaatgg tagtcatcct gcatgtttac ccactgacgc
    42841 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    42901 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    42961 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    43021 gttatatccg taggttaagc caactgatgg gcaacctgcc gcttttgctg cctgaatatc
    43081 attgcgtgag tcgccaacaa acagcatctg ttgtggagca attcccatcc gctcagccac
    43141 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcaccac caatcaccac
    43201 gctgaagtat ttagcgatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    43261 gttggtgacc aggcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac
    43321 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt
    43381 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttacccat
    43441 tgttttacgc tgagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac
    43501 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag
    43561 cgcatacagc gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc
    43621 atcaagatca aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca
    43681 gttcactgcg catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac
    43741 cggcgacgaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    43801 caccgtccac ttccagacga atgtcaaagc cagactcgtc gatacgacga cgtacttcgc
    43861 gcagtttatc cagtgtttgc ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca
    43921 tcagcaggat cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg
    43981 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    44041 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    44101 gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccat
    44161 agttacgcaa ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat
    44221 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    44281 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    44341 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    44401 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    44461 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    44521 cacaggaata tggcgatcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    44581 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggcgcata
    44641 aggcggatcg caatagacga cggatgcatc atctgcacgc gccatgctat cggcgtaaga
    44701 ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    44761 gaaatagggt tttttgtagc ggccaaacgg cacattaaac tcaccgcgca gattgtaacg
    44821 acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    44881 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    44941 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    45001 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    45061 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    45121 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    45181 cttcttcatg ctgactaact aattacacct tctccggctg tggagaaagc tccgacagca
    45241 tccagcgctt caggcaactc ccgcaacgat aaaccattgc gggagataac cttgattact
    45301 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tggacatctg
    45361 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    45421 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    45481 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    45541 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    45601 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    45661 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggagcagctg
    45721 gttcagtacg cttcggcgtc tgtgctaccg gcttcggttc cgttttcacg gttgcttgcg
    45781 gttttttcgg ttcaatcacc gcctgctgac gagctagacg cgtagtggcc ggacgttcag
    45841 cagtttgcgt tttcgccgtg tcacgcgatg cattgccatt gcgtactggc gcgacagttg
    45901 ctggttcagt cggcaatgta gaattgaccg ccacattgtt caactgttgc tgattttgtg
    45961 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    46021 ccaccggggt ttgcccttga gtcggcgtag aagagatcgg tggcagagaa acatcctgct
    46081 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    46141 cattgccagc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg
    46201 agggggcttt tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga
    46261 cgcccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg
    46321 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt
    46381 cgatttccgg ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    46441 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    46501 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    46561 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    46621 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    46681 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    46741 ctcttaccaa ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca
    46801 cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac
    46861 ccagcccgct tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    46921 cgttccgacg tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa
    46981 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    47041 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    47101 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    47161 tccagccagt taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    47221 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    47281 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    47341 ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca
    47401 ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc
    47461 gccaccagcg tagtatcgcg accatgcggt ttttgtaaca acgccgtaaa gacggtatcg
    47521 agtacagcca ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    47581 cccgcctgtt caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgttg
    47641 gtgaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    47701 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    47761 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    47821 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    47881 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    47941 ccagaacttc acgtggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    48001 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    48061 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    48121 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    48181 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    48241 cgtaaaattc catattgagt tgttgagcta actggcgccc aatagtgctt tttccggcac
    48301 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    48361 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    48421 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    48481 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    48541 ccgctctggc gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    48601 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    48661 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcgtaaaaac
    48721 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    48781 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    48841 tcgccaagca acggcacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    48901 agcgccaacg tttctccgct tttgacctcg acctgcgttt cgatctcctg cttatcaatc
    48961 gccagcactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    49021 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggact
    49081 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacttg atatggaatt
    49141 tcgctcccct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactagcg
    49201 ataatatcca gctgctgttt ttgttcgagc gcagaaagct caagatccag caaacgtccg
    49261 ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgctg
    49321 ccaagcgtgg tgacttgccc aacgccacca gcttgttgcg catcggccag cgtccatttc
    49381 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    49441 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gggcgcttaa cgccgttttg
    49501 ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggtactc
    49561 agtagcttct ccccagcttt cgccagctct ccggcgtcgg cgtattgcag agtaatattg
    49621 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcttcctg acgggcgata
    49681 ttgtcattct gccaggcaat ggaatgcact gagagaatgt tgccctcctg acgcgttatc
    49741 agtccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt
    49801 aacgacaccg taccgctgac gtctggcgac accaccaggt tcaacttctc ctgttcagcc
    49861 agcgcctgca acacctgagc taccggaaca tcatccacca tcagcgtcac tttttgcggc
    49921 tttgccgcct gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt
    49981 tgtatctcct tgccgttgcc acaaccatcg tggcggttcg cagtttgtcc cggtacccag
    50041 cgttagtacg tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg
    50101 ctgcacccgt cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg
    50161 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    50221 cggtttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    50281 gcgtttaacc ctcatgcggc gtctccagtt gtagcgtgaa caaaagatca tcaccttcca
    50341 cgcttaatga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    50401 gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccaga
    50461 aaaccagttg tgcgccgggc aactgaaaat ccagtggcga aaagggcagc gttttttctt
    50521 cgctgaaggg agtggtgtca accaggcgat acagtgtggc ccactgctga tgatgactta
    50581 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgacaga aaaatgagcg
    50641 taactaacat cagcaaccag actgcccagc aaagctggcg gagacggggg gatgtggcga
    50701 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    50761 cagcgtccct gcgcatcctg ctgcgtggcc ccccgctgat tgagatgaaa agatgcatcc
    50821 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtaag ccccttgatc
    50881 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    50941 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgcctttgc
    51001 aaacgctgct cacgaatttg ttggcgctcc agcaaacgtg gtttcgttat ctgtaagctg
    51061 cgggcgagtt gttgttcccc ctgaagcaaa acggcgtcta cgcgagcttc ggcgctgctt
    51121 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    51181 aaccagaaac gaagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    51241 gggttcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccacctg
    51301 gcggcggtaa cggcggctga cgaacagaaa cggcctccca gggatcaaat ccgccttcgc
    51361 cacatatcgc gacgctttcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    51421 tcatccccac cgccagtttg cgcccccagc gatagcgcgt cgcccacagc cactgttcgt
    51481 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    51541 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agagtaagca
    51601 gcgttgccag ctctttgctt tgcgcggcag tcacgttata ggcgggactg agtgagtctt
    51661 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgagcc atcgtcccgg
    51721 acagccaggc cgtttgctcc cgctcaccga gggacatcga cgggcgcgga aatgaccgct
    51781 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    51841 tccacggtaa caacgtttta gccagccgct gcgcatcaac aatccgccca tctttgataa
    51901 tgtcgtgcgc caacggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca
    51961 caatagcaac cgctaccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    52021 atgccattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    52081 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    52141 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga
    52201 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtagcgaca
    52261 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    52321 gctcaatacg gtgagaaacg tcgtattccg gttacgctgg atcaaatccc gccggagatg
    52381 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg
    52441 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggca
    52501 agtaccatta cccagcagct ggcgagaaac ttcttcctca gtccagaacg cacgctgatg
    52561 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa
    52621 atcctcgagc tttatctgaa caagatttac ctcggttacc gcgcctatgg tgtcggtgct
    52681 gcggcacaag tctattacgg gaaaacggtc gaccaactga cgctgaacga aatggctgtg
    52741 atagccgggc tgccgaaagc gccttccact ttcaacccgc tctactcgat ggatcgtgcc
    52801 gtcgcgcggc gtaacgtcgt gctatcgcgg atgctggatg aaggatatat cacccagcaa
    52861 cagttcgatc agacacgcac tgaggcgatt aacgctaact atcacgcgcc ggagattgct
    52921 ttttctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    52981 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    53041 gcacagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    53101 ccggcaaatg tgctgtggaa agtgggcgag tcggtgtggg ataacaacaa gattaccgat
    53161 acgctgaagg cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    53221 cctcaggaag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    53281 gttcgctggg cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg
    53341 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    53401 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaacggtgcc
    53461 gttatggcgt tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    53521 gcactgcgtc aggtaggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    53581 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    53641 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ccggtccaat tcgcttacgt
    53701 caggggctgg gtcagtcgaa aaacgtggtg atggtacgcg caatgcgggc gatgggcgtc
    53761 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    53821 gaatcgctgg cgctgggttc agcgtccttc actccaatgc aggtggcgcg cggctacgcg
    53881 gtcatggcga acggcggctt cctggtggac ccgtggttta tcagcaaaat tgaaaacgat
    53941 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg tgatattccg
    54001 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    54061 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    54121 caggcattag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    54181 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgagcc aggctggcag
    54241 ggtactggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    54301 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    54361 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    54421 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    54481 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gccgccgggt
    54541 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    54601 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    54661 accattatcg ataatggcga agcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    54721 tttttaagtt tgctgacaaa gtgcactttg tttataccgg atacggcgtg aacactttac
    54781 ccgtctagca acatctagaa aaatcaatat attgcagaaa cttcgtaagc ctgataagca
    54841 tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt caaccattcg cgcacgagga
    54901 acagcgcgct gacattacgc gcttcgttga agtcagggtc ttccagcaaa tccatcatat
    54961 gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc atcgccttcc agcgattccg
    55021 ggtagagatc ttgcgctacc acgatattca ttttgctgga aaagtaagac ggtgccatgc
    55081 tgagcttctt caaaaaagtc agatcgttcg caccaaatcc aacctcttct tttagctcac
    55141 ggttagcggc ttcgaagacg ctttcacccg gatcaattaa tcctttcgaa aaacctaatt
    55201 cgtaggattc agttcccact gcatattcgc ggatcaggat caggtgatcg tccacaatcg
    55261 gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca cgccgcacgc
    55321 cattgctgaa ctccagatcc acgctctcga cggtaaacag tcgggaacgg gctacagttt
    55381 caacattcag aatggtgggt ttttgtaatg atttgctcat cgtgggatct atgctgtgaa
    55441 atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc atgttattta
    55501 catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa taggttgaaa
    55561 taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc agcggttact
    55621 gtgatgtgct taatgatgct caagttaaac tccacgcttg ccgatagcca accgcagaat
    55681 catgtattgt gtccggtgcg actgaccacg cctgacagac taagtaagat ggggaaagca
    55741 tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt gcgggatggc
    55801 tgataaaagt gcgatccaga cggcgtcagc tgccctggac caacgccttc gcggatgcgc
    55861 aaacgcgtaa actcacacct gaagaacgta gcgccgttga aaattatctt gagagcctga
    55921 cgcaggtatt acaggtgcct ggcccaacgg gagccagcgc ggcaccgatc tctctggcgc
    55981 tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacacgt tacggcatct
    56041 ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc cacctgcccc
    56101 ccttctggga acagtacatc aacgatgaga ataccgttga actgattcat accgattcgc
    56161 tgccgttggt tatttcactc aacggtcata cgctgcagga gtacatgcag gaaacgcgcg
    56221 gctatgcctt gcaacctgtt ccgtccacgc aggcgtcgat tcgcggggaa gagagtgagc
    56281 aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga atatgcgctg agtcgtccgc
    56341 gcgggctgcg tgaagcgttg ctgatcgtcg cctccttcct gatgttcttt ttctgcctga
    56401 ttaccccgga tgtatttgtt ccgtggctgg caggtggcgc gttactgctg ctgggcgcag
    56461 gtctgtgggg gctattcgcc cccccggcaa aatcctccct gcgggaaatt cattgtctgc
    56521 gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag atcaacaata
    56581 tttcactcgg tattatcgac ctggtctatc ccgcacactg gcagccatac attgctcagg
    56641 atctcggtca acaaaccgat atcgatatct atctcgaccg ccatgtagtg cgtcagggac
    56701 gatatctttc gctgcatgat gaagtaaaaa actttccgtt acagcactgg ctgcgcagta
    56761 cgattatcgc tgcgggctcg ctgctggtgc tgtttatgct gttattctgg atcccgctgg
    56821 atatgccgct gaaattcact ctctcatgga tgaaaggcgc gcagaccatt gaagccacca
    56881 gcgtaaaaca actggctgac gctggcgtgc gggtaggcga tacattgcgt attagtggta
    56941 cgggaatgtg taatattcgc acttccggaa cctggagcgc gaaaaccaat tcaccttttt
    57001 taccgtttga ctgctcgcag atcatctgga atgacgcccg ctcattacca ttaccagaat
    57061 ctgagctggt caacaaagcg acggcattga ctgaagcagt taatcgccag ctgcacccta
    57121 aaccggaaga tgaatctcgc gtcagtgcct cattacgttc agcgattcaa aaatccggca
    57181 tggtattgat tgatgatttt ggcgacattg tactgaagac agcggatctg tgttctgcca
    57241 aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt aaagactggg
    57301 acgcgctggt aaaacgcgcc aacgccggga agctcgatgg cgtgaatgtg ttattacgcc
    57361 cggtgagtgc ggaatcgctg gataacctgg tggcaacctc caccgcgccg tttatcacgc
    57421 atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcgga ttcctgattg
    57481 tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca ctttacgact
    57541 acccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg atgcatacgc
    57601 cgtttaacgc cgagggtatc gtcacaaaaa tcttcactga cgccaatggt acgcagcata
    57661 taggccttca tccgatcccg gatcgttccg gcctgtggcg ctatctcagc accacactgc
    57721 tgctactgac gatgctgggt agcgccattt ataatggcgt acaggcctgg cggcgttacc
    57781 agcgtcatcg cactcgcatg atgaagattc aggcctatta tgaaagctgc ctgaacccgc
    57841 aactgatcac cccttcagaa agccttatcg aataacacgt ttgcgcggca ggttatgcta
    57901 ccctgtcgcg caaattgctt cactctggag attttcctca tgcatatcaa cattgcctgg
    57961 caggacgtag ataccgttct gctggatatg gacggcacgt tgctcgacct cgccttcgat
    58021 aactatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg ggttacgcca
    58081 caggaagcga tggaatttat gcgccagcaa tatcacgacg tacagcatac gctaaactgg
    58141 tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat gaccaccgag
    58201 atgggaccgc gtgccgtact gcgtgaagat accattccgt ttcttgaggc actgaaagcc
    58261 agcggtaagc agcgaatttt gctcaccaat gcgcatccgc acaacctggc ggtaaaactt
    58321 gagcataccg gtctggacgc acaccttgat ttattacttt ccacccacac atttggttat
    58381 ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct gaaagctgaa
    58441 agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca atttggtatt
    58501 cgttactgcc tcggcgtgac taatcctgac tcagggattg ccgagaaaca gtatcaacgc
    58561 catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag gagaggccat
    58621 gaaagaaaaa cctgctgttg aggttcgact ggataaatgg ctatgggctg cccgttttta
    58681 taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt aaggtgcatt acaacgggca
    58741 gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc gccagggaaa
    58801 tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg ccagcgaggc
    58861 agccttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgctggc
    58921 acgtaaactt aatgccttaa ccatgccgca cccggaccga cgcccggaca aaaaagagcg
    58981 ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg caagagagat
    59041 gattatgccg caacatgacc aattacatcg ctatctgttt gaaaactttg ccgtgcgcgg
    59101 cgaactggta accgtttcgg aaaccctgca acagatcctt gagaaccacg attatccgca
    59161 gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa ccgctacgct
    59221 gaagtttgat ggtgatatca ccgtacagct gcagggcgac ggtccgatga atctggcagt
    59281 tattaacggt aacaataacc agcaaatgcg cggtgtggcg cgcgtgcagg gcgaaattcc
    59341 agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca ccattacccc
    59401 gagcgaaggc gaacgctatc agggcgtagt tggtctggaa ggtgataccc tggcggcctg
    59461 cctggaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg
    59521 cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg caggtaatgc ctgcgcaaaa
    59581 tgcccagcag gacgacttcg accacctggc gacgctaacc gaaaccatca aaaccgaaga
    59641 actgctgacc ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac
    59701 ggtttacgat ccgcaggatg tggagttcaa atgcacctgc tcgcgtgaac gttgcgccga
    59761 tgcgctgaaa acgctgcctg atgaagaagt cgatagcatc ctggcggaag atggcgaaat
    59821 tgacatgcat tgtgattact gcggtaacca ctatctgttc aatgcgatgg atattgctga
    59881 aatccgcaac aacgcgtctc cggcagatcc gcaagttcat taatgagtat gtccggcaga
    59941 gagagctctg ccggatttta cgactaatac acctgatgcg ctttgtcact cattcagatt
    60001 cacatccagc ctttcaccat ccactacaag atcgcgccac gctttaggct tcgttcccag
    60061 ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atatcacgcg gaagtaaact
    60121 catcgtaagc tcttgccatt ctttcttgtc aggatcaaag ccatagacaa tgtatctttc
    60181 atcatcaaag gcaaagagga tccgctctgc cctgccatca ctattcagat cttgctcgac
    60241 cagtacgcag gcgtcttttt caatacaggt catcacgtta tagcgatcct ggattaaggc
    60301 cgaccagaat gtcgcatccg gtttaccaga accaggggca attaacacat tttcggctaa
    60361 tactttttcc gatatctgtt gctgaagatg ttgctctcca tctaacgcca tcagtagatc
    60421 ccgcgcacgt ttcgggtctt tcatatactc ggcatcgctt ttcagcgact caagcgccgc
    60481 acgtccatag cgaccgctgt gttcgagcat gtagatagtt acctggtctg gcgtgttttt
    60541 gccgctctga taacgcgcca tatgactgtt cacactaatg cgcatactgt ccagcaccgg
    60601 cgaattaaga agcaccagta ttaccaacac caataacgaa accgcaaggt tcactttgcc
    60661 ctgaagaaca aggggatttt gccctttacg ccagacgata ctgacaaaat accccagcga
    60721 ccataccagt aacaccagca ccgccagcgc gccctgcaag cggtcgacag tccagccgta
    60781 ctgagcgacc cgcagccata acgcccaggc ggcgatgaac acatacagcg gagcaaccag
    60841 cagagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagtgacg ctttttgcgg
    60901 atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca gcaacccggc
    60961 ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagggtga tgataaacat
    61021 cagggttagc aatgatacta acggcagcaa ccccgtggcg attagcgtga acaacttttg
    61081 aatagagtcg attaaacgtg actgtgttcg cgcgaggatc accgccagcg cggtaaccag
    61141 acctaatgta agatacataa accagtcggt tgcaaaaaag agcgtattga aaaacgtgat
    61201 gccaacaagt ttaaacaact caccccacag taaaagcacc agccacgtca ggccattggc
    61261 gagaaaaata acctgtaata taagtacgtt atgccatact gactggtaaa aatagcaata
    61321 gcggctactg tcattgcgga tacgaaggct ttgttgtatc cacggtagca acaacattgc
    61381 catcagcagc agatagcagc caaaatccca aagcgccttt tcagctctcc aggggttcat
    61441 gccatcagtt tgccacttca accagccgct catccccagc gtggcaataa acaccagcgc
    61501 cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta aaacggatga
    61561 aaacgcaacg gtcgcaggca cgccataaac aatccaactg tgattttttc cggcaagcca
    61621 ggcgatcagc agatagcaaa ccagcccctg aagtaatccg gtagcaatca tcccccagcg
    61681 tgttgcgggt gaaagttcaa cgttatccat gttggttatc cagaatcaaa aggtgggtta
    61741 attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa aaaagttagc
    61801 gtggtgaatc gatactttac cggttgaatt tgcatcaatt tcattcagga atgcgattcc
    61861 actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc
    61921 taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg
    61981 tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt aacaccccca
    62041 aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac
    62101 aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt
    62161 tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat
    62221 gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt
    62281 cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac tttctggtgg
    62341 gcagacaaag gcaaaggtaa gaacgataac aaacctctct ctccggaaac ctggcagcat
    62401 ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgccttc
    62461 tgtggtgcga atccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag
    62521 gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc aggtttcaaa
    62581 ccagacttta tcgttatgaa cggcgcgaag tgcactaacc ctcagtggaa agaacagggt
    62641 ctgaactctg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct gattggcggc
    62701 acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta cctgctgccg
    62761 ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gcgagaaagg cgatgttgcg
    62821 gtgttcttcg gcctttccgg cactggtaaa accacccttt ccaccgaccc gaaacgtcgc
    62881 ctgattggcg atgacgaaca cggctgggac gatgacggtg tgtttaactt cgaaggcggc
    62941 tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta caatgctatc
    63001 cgtcgtgatg cgttgctgga aaacgtcacc gtgcgtgaag atggcactat cgactttgat
    63061 gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat cgataacatc
    63121 gttaagccgg tttcaaaagc gggtcacgca acgaaagtta tcttcctgac cgcagacgcc
    63181 tttggtgtgc tgccgccggt ttctcgtctg actgccgatc aaacccagta ccacttcctc
    63241 tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc gacgccaacc
    63301 ttctccgctt gcttcggcgc ggcattcctg tcgctgcacc cgactcagta cgcagaagtg
    63361 ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc tggttaacac tggctggaac
    63421 ggcactggca aacgtatctc gattaaagat acccgcgcca ttatcgacgc tatcctcaac
    63481 ggttcgctgg ataatgcaga aaccttcact ctgccgatgt ttaacctggc gatcccaacc
    63541 gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc ttccccggaa
    63601 cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt tgataaatac
    63661 accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaaactgta atgacttgaa
    63721 gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc ttcgcctccc
    63781 gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt accggcacag gcagccaggc
    63841 gcgaatggaa agcccgcccc gctcgctggt gccaagctcc agcatcccgt tatggttatc
    63901 cacgatacgc tgcacaatcg ccagccctaa tcccgtgccg ctaatggtgc gtgcactgtc
    63961 gccacgaaca aacggctgga aaaggtgctt acgttgttcc ggcgcaattc ccggaccgtc
    64021 atcttccacc tggaaccagg cgcgattcgg ctccgtcccg ctgctgactt tgatccagcc
    64081 attgccgtaa cgggcggcgt tgaccaccat attcgccacc gcgcgtttga tcgacagcgg
    64141 gtgcattttc acttcaatgc tgccggggta aagcgcggtt tcaatttccc gctcatagcc
    64201 actttcggca gcaataacct caccgagtac tgcgttgaga tccgccattt ccatcggcat
    64261 ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc actcttcgat
    64321 atctttattg atcgattctg ccaggtagcc atcctgctcg ctcatcatct cggtcgccag
    64381 gcgaatacgc gtcagcggtg tacgcaagtc gtgacttacc cccgccatca gcagcgtgcg
    64441 gtcatccgcc agttgcttaa caccagccgc catatggtta aaggcgcggg taacggaacg
    64501 cacctcggac gcgccatact cacgcagcgg cggcggaata atccctttac cgacctgcaa
    64561 agcagcatgt tcgagatcga ccaacggtcg gttctggata cgaataaaca gccacgcccc
    64621 gcctatcgcc aatagcataa tcgccaacgt atagcggaac agcggagaga aatcgccctg
    64681 atgaatttcg gtcagcggca cgcgtaccca gatattgggc gacagccaag ttttcagcca
    64741 gacgacaggc gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc ccagttgctg
    64801 cgccatctga tggcttaaga attcatagtg ttgcgcccaa cgcagacctg cctcttcggc
    64861 agcctcgttg gagtagagag agatccccag ctcacggtag atctcccgac ggaaagcggg
    64921 aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca acatacgcac
    64981 ttcgtacgcg aggactttat taaactgctg gaggctcggc aaaatcgcga agttcagcac
    65041 caccagataa gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca ataacgtacg
    65101 ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc gtccggtaca
    65161 aagacgtagc ccaagcccca aacggtctga atgtaacgcg gatgcgctgg atcttcttcc
    65221 accatgcggc gcagacgcga aatctgcacg tcgatggagc gttccattgc ggaatattca
    65281 cgaccacggg caaggttcat cagcttatcg cgggagagcg gctcacgcgg atggctgacc
    65341 agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca tcggctcgtc ttcgcggaac
    65401 atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag caattaccgc ctcttcctgt
    65461 gacggtgcgc ctggtagctc gttcgcctga cgacgcagca ccgcacggat acgggccagc
    65521 agttcacgcg ggttaaacgg ttttggaatg taatcgtcag cgccaatctc caggccaacg
    65581 atacggtcca cttcttcccc tttcgccgtc accataatga tcggcatcgg gttgctctga
    65641 ctacgaagac gtcggcaaat cgacaagcca tcttcaccag gtaacatcaa atccagtacc
    65701 ataagatgga aagattcacg agtcagcagg cgatccatct gttctgcatt agcgacgctt
    65761 cgaacctgga agccttgttc ggtgagataa cgctccagca gcgcgcgcag gcgcatgtcg
    65821 tcatcgacca ccagaatctt gtagttctct tgcattgttt gtactcccaa aggttcgcaa
    65881 caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa atctggtatg
    65941 aatttcagcc taaattgtta caaagcatat taaacagcag cttaagtata caatttattc
    66001 ggcgaaacat tattgattct gttgatatga tcacgttata cccaatgtgc gcattatcaa
    66061 acagacaaag ggaatcaacg agatgaaaac gcccctggtt acccgggaag ggtatgaaaa
    66121 actcaaacaa gagcttaatt atctctggcg tgaagaacgc ccggaggtca caaaaaaggt
    66181 gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt ataataaaaa
    66241 gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg aaaatctcaa
    66301 aatcgtcgat tactcccctc agcaggaagg caaagtcttt tttggcgcgt gggtggagat
    66361 tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg aaatttttgg
    66421 ccgtaaagat tacatctcta tcgattcccc gatggcccgc gcattgctga aaaaagaagt
    66481 cggcgatctg gcggtggtga atacccctgc cggggaagcg agctggtatg ttaatgctat
    66541 cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc cgatggctgg catttttgcc
    66601 agccagtccg tataactatc ccctgatttt tgatccgaaa agatgaactc aaaccatgat
    66661 gaatgattcg ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg aacaggttga
    66721 cgctgccgtt cgcctgcttg acgaagggaa taccgtgcct tttattgcac gttatcgtaa
    66781 ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc gtctgagcta
    66841 tctgcgcgag ctggaagaga gacgtcaggc gatcctcaag tccatttccg agcaaggcaa
    66901 actcaccgat gatctggcga aggccatcaa cgccacctta agcaaaaccg aactcgaaga
    66961 cctctacctg ccctacaaac ctaaacgccg cacccgcggg caaatcgcca ttgaagcagg
    67021 gcttgagccg ttggctgacc tgctgtggag cgatccgtca cacacgccag aagtcgccgc
    67081 tgcgcaatat attgatgcag ataaaggcgt ggcagatacc aaagccgcgc tggacggcgc
    67141 gcgctatatc ctgatggaac ggtttgccga agatgccgcg ctgctggcga aagtgcgtga
    67201 ttatctgtgg aagaacgcgc atttggtttc gacggtggtg agcggtaaag aagaggaagg
    67261 ggcgaaattc cgcgactatt tcgatcatca cgaaccgttg tccacggtgc cttctcaccg
    67321 cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc cagctttcgc tgaatgccga
    67381 tccgcagttc gatgagccgc caaaagagag ctattgcgag caaatcatca tggatcatct
    67441 cggcctgcgc ctgaacaatg ctccggcgga tagctggcgc aaaggcgtgg tgagctggac
    67501 ctggcgcatc aaggtgctga tgcatctgga aaccgaactg atgggcaccg tgcgcgaacg
    67561 cgcggaagat gaagcaatca acgtctttgc ccgtaacctg cacgatctgc tgatggcggc
    67621 ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt ctgcgtactg gggtaaaagt
    67681 ggcggtggtc gatgccactg gcaaactggt agcgaccgat accatttacc cgcacaccgg
    67741 acaagccgca aaagcagcga tgactgttgc tgcgctgtgt gaaaagcata acgttgaact
    67801 ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa cgtttctatc tcgacgtgca
    67861 gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc agcgaagctg gcgcgtcggt
    67921 ttactcggct tccgagctgg cagcgcagga gttcccggat ctcgacgttt cgctgcgtgg
    67981 cgcggtttct atcgcccgcc gtttgcagga tccgctggcg gagctggtga aaatcgatcc
    68041 gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac tggcccgcaa
    68101 actggatgca gtagtagaag actgcgtaaa cgccgttggc gtcgatctca acaccgcttc
    68161 tgttccgcta ttaactcgcg tggcgggcct gacgcgcatg atggcgcaaa acatcgtggc
    68221 ctggcgcgat gagaacggcc agttccagaa ccgccagcaa ctgctgaaag tcagccgtct
    68281 ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc attaaccacg gtgacaaccc
    68341 gctggacgcc tctaccgttc acccggaagc ctatccggtg gtggaacgca ttctggcagc
    68401 aacacagcag gcactgaaag atctgatggg taacagcagc gaactgcgta acctgaaagc
    68461 gtctgacttt actgatgaaa aattcggtgt gccgacagta actgacatca tcaaagagct
    68521 ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg ccgatggcgt
    68581 cgagacaatg aacgacctgc aaccgggtat gatcctcgaa ggcgctgtca ccaacgtcac
    68641 caactttggc gcgtttgtcg atatcggcgt gcatcaggac ggcctggttc acatctcttc
    68701 attgtcgaac aagtttgtgg aagatccgca taccgtggtg aaagcgggcg acattgtgaa
    68761 ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc gccctgacta tgcgtctgga
    68821 cgagcagcct ggcgaaacca actctcgtcg cggcggcggt aacgatcgcc cgcaaaacaa
    68881 ccgcccggca gccaaaccac gcggtcgtga agcgcagcct gccggtaaca gcgcgatgat
    68941 ggacgcgctg gcggcagcaa tgggcaaaaa acgttaaacg cccggactgg cccctaaacc
    69001 tgcgcgataa ggcgttcacg ccgcatctgg caacgaacgc cttgcctgac ataaaagtgc
    69061 cggagaatat ctccggcata tttattccac agccaaactc acaatatatt tcgttaatat
    69121 ttatcacttc attaacaact gaaaccttaa ttaaacatta gccagtcctg gtaattcgct
    69181 attcgaatta tattttcgct gcgatataac cttgagccac atcaacattg agtcagatta
    69241 ttattcaaac caacattcgc acacatttta agtattgctg atagaaacca ttctcattat
    69301 cattgtactg ttgattattt aatctctcct tcgttggcaa atcatctggt ctcatgtcgc
    69361 tgtcaaacgc cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa
    69421 tacactccag atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat
    69481 cgccaaaaac tgctttctct tggcatgtta cctggctcct cttttaatgt ggtgcgcgtc
    69541 gctccactcg gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa
    69601 aaagatctgg ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat
    69661 gaaaaaatta accataggct taattggtaa tccaaattct ggcaagacaa ccttatttaa
    69721 ccagctcact ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa
    69781 agaagggcaa ttctccacca ccgatcatca ggtcacgctg gtggacctgc ccggcaccta
    69841 ttctctgacc accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactacat
    69901 tttgagtggc gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa
    69961 cctgtacctg acgctacaac tgctggaact cggcattccc tgcatcgtgg cactgaacat
    70021 gctcgacatt gccgagaagc aaaatattcg tattgaaatt gatgctctgt cggcgcgtct
    70081 tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct
    70141 ggcgattgat cgctataaag ctaacgagaa tgtggaattg gtgcattacg cacagccgct
    70201 gctcaacgaa gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg
    70261 tcgctggctg ggcctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga
    70321 agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt aatgagatgg acgatccggc
    70381 gctgcacatt gccgatgcgc gttaccagtg cattgctgcc atctgtgatg tggtaagcaa
    70441 caccctgacg gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg
    70501 tttcctcggt ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa
    70561 catcggcggg gcgttacagc cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg
    70621 tattcaatgg attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca
    70681 gggcctgggt ggcggtatta acaccgtgct gccactggtg ccgcagatcg gcatgatgta
    70741 cctgttcctc tccttccttg aggactccgg ctatatggcg cgtgcggcgt ttgtgatgga
    70801 ccgcctgatg caggcgctgg gcttgccggg gaaatccttt gtgccgctga tcgtcggttt
    70861 cggttgtaac gtaccgtcgg taatgggtgc acgtacactt gatgcaccgc gtgaacgtct
    70921 gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt
    70981 attcgcggct gccttcttcg ggcagaacgg tgcgctggcg gtcttctcgc tgtatatgct
    71041 cggtatcgtg atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga
    71101 agcgacgccg tttgtcatgg agctgccggt ctaccatgta ccacacgtta aaagcctgat
    71161 tatccagacc tggcagcgcc tgaaaggctt cgttctgcgt gctggtaaag tgattatcat
    71221 cgtcagcatt ttcctgagcg ctttcaacag cttctcgttg agcgggaaaa tcgtcgataa
    71281 catcaacgac tcggcgctgg cttccgtcag ccgggtgatc accccggtct tcaagccgat
    71341 tggcgtgcat gaagataact ggcaggcaac agttggcctg tttacgggtg caatggcgaa
    71401 agaagtggtg gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga
    71461 gttcaatccg gcggaattta acctcggtga agagttgttc agcgcggtag atgaaacctg
    71521 gcagagcctg aaagacacct tcagccttag cgtactgatg aaccccattg aagctagcaa
    71581 aggcgacggc gaaatgggta ccggggcgat gggtgtgatg gatcagaaat tcggtagcgc
    71641 agctgccgca tacagctacc tgattttcgt cctgctgtat gtaccgtgca tctcggtgat
    71701 gggagccatc gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct
    71761 gaatatcgct tactcgctgg caacattgtt ctatcaggtc gccagctaca gccagcatcc
    71821 aacttacagc ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg ttatcggtct
    71881 gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag
    71941 tagttgctgc gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggtg
    72001 cgtgatttgt tggcgttacg gggccgtatg gaagcggccc agataagcaa gacattgaac
    72061 actccacagc caatgattaa cgccatgctg caacaactgg aaagtatggg caaagccgtg
    72121 cggattcagg aagaacctga cggctgcctc tccggcagtt gtaaaagctg cccggaagga
    72181 aaagcctgtc tgcgcgagtg gtgggcgctg cgttaaccat actccatcgc ctgttttgaa
    72241 aagcccggta tgcgtttgca tccgggcttt tttgcgtgcg gctttccata aaaatgcaat
    72301 tcttgcagcg cgacgtaagt tcctctgaaa gcgtctcgca gggatgaaaa ctggctaatg
    72361 cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat
    72421 gagcaaaaag cagagttcca ccccacacga tgcgctgttc aaactatttt tacgccaacc
    72481 ggagacggca cgcgattttc ttgcgtttca tttaccggca cccattcacg cgctctgtga
    72541 tatgaaaacc ctcaagctgg agtcgagcag ctttattgat gacgatctgc gtgaaagcta
    72601 ttccgacgtg ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat
    72661 tgaacatcaa agcacctcaa acaaactgat cgcattccgc atgatgcgtt acgccattgc
    72721 cgcaatgcaa aatcaccttg atgccggata caaaacgttg ccgatggtgg tgccattgtt
    72781 gttttatcac ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc
    72841 cgatcccaac ctggcaaggc agctctatgc ctctgcattt ccgctgattg atatcaccgt
    72901 catgcctgat gatgaaatca tgcagcaccg acgcatggcg ctgctggagt taattcaaaa
    72961 acatattcgt caacgcgacc tgatggggct ggtagaacaa atggcctgct tattaagtag
    73021 tggatacgct aatgacagac aaatcaaagg gctgtttaat tacatactgc aaaccggcga
    73081 tgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga aacacaagga
    73141 gagtttaatg actattgcgg aaagattgcg gcaggagggg gaacaatcca aagccttgca
    73201 tatagccaaa ataatgcttg aatccggagt ccctcttgca gacatcatgc gctttaccgg
    73261 gctgtcagaa gaagagttgg ctgcggcgag tcagtaaagt tctgtctcgc cattcaaaag
    73321 tgcctacacc ctctgcttca acgccaccag caggtgacaa aactcgaccg gatgcgaaat
    73381 aaacggcgca tgggccgctt tggcgaagat atacgactcg ctgtgcggcc agagtttatc
    73441 cagcatcggc accactttgc gcggcaccag accgtcgaga taaccataca gtcgcaaaaa
    73501 cggcatgggt acgttttgca gcggctggcg gagatcgacc gttttcagga tttccagccc
    73561 accattgagc acgtcaacct ccggcatcgg taacgccaga acggttttct tcaacgcccg
    73621 cgcatcctga cgcgccgttt cagtacccat ggtttgtaac gcaaggaacc gttccaccgt
    73681 acgctgaaaa tcatcactga gttgctgctg aaatcccgcc agcacgtccg gttttatccc
    73741 cggccactct tcacgagcgc tgaaacacgg cgacgacgcc acggtgacca gcgcctgaac
    73801 acgctcggga tgggttaacg caatctggct tgccaccagc ccgcccagac tccagcctaa
    73861 ccaaatggct ttatcaggtg cctgtcgcag cacggcttcg gccatatcag caagtgacat
    73921 cgcaccaaat ccccggctac gcccgaagcc gggcaggtca acaagatgca gcgtaaaatg
    73981 cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca ttcagtcccc atccgtgcag
    74041 cagcacaaga tgaacattcc cctgaccttt ggtctgccac cagatgttat tcatccgcta
    74101 ttgttctctt ttgacttaca aggatgaaca tatgctaaca gtaccgggat tatgctggct
    74161 atgccgaatg ccgctggctt taggtcattg ggggatttgt tcggtctgct cacgcgccgc
    74221 ccgtacagat aaaacgttat gcccacaatg tggattaccc gccacacact cccatcttcc
    74281 ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta
    74341 tgcgccgccg ttaagtctgc tcatccacca gcttaagttt tcccgccgca gcgaaatcgc
    74401 cagcgccctg tcacgtctgt tactattgga agtcttacac gctcgtcgca ccaccgggtt
    74461 gcaattgccg gatcgtatca tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg
    74521 atttaatcag agcgatttgc tgtgtcagcc gttatcacgc tggttgcact gccaatggga
    74581 tagcgaagcc atcacacgta cacgggccac tgcgacccag cattttctta gcgcccggct
    74641 acgcaggcgc aacctgaaaa atgcctttcg ccttgaattg cctgtgcaag gtcgccatat
    74701 ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc gcagagattg cgcagttgct
    74761 tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct
    74821 cgatgatggg cgtattataa ccaactaaaa tagtcaacta ttaggccatt actatgatcc
    74881 gtatttccga tgctgcacaa gcgcactttg ccaaactgct ggcaaatcag gaagaaggga
    74941 cacaaatccg cgtatttgtg attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt
    75001 attgtccgcc ggatgccgtg gaagccaccg acaccgccct gaaatttgac ctgctgaccg
    75061 cgtatgttga tgagttaagc gcaccatacc tggaagatgc agagatcgat tttgttaccg
    75121 accagttggg ttctcagcta acactgaaag cgcctaacgc caaaatgcgt aaagtggcag
    75181 acgatgcacc actgatggag cgcgtggagt atatgctgca gtcgcagatc aacccacagc
    75241 ttgctggtca cggtggccgc gtttcactga tggaaatcac cgaagacggt tacgccattc
    75301 tgcaatttgg cggcggctgt aacggttgtt ccatggtcga tgtgacgctg aaagaaggga
    75361 tcgagaagca gctactaaac gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac
    75421 accagcgcgg cgaacactcc tactactaag ttatcccctc ttttgtagat tgcccgatgc
    75481 gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc cagacgttgg cgcagcattg
    75541 ctttggcaaa tccctccctt cgtttccccg cgataatatg accaacctct cataatttaa
    75601 atttaccccg ctctggtgat tctcaaacgc cagatgttac ccgtatcatt cacatgggta
    75661 ccaaacatac tcctgacatc tgactacaat aattagtttt agtgggtatc agtcgtggtg
    75721 ccgcaatatc tctgttcccg attgggataa ttagagtttg tcgtcagaaa attgacgtta
    75781 cccataacaa atgaaaggcc aggtaaatca tgccattagt cattgttgct atcggtgtaa
    75841 tcttgttgtt gctcctgatg atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg
    75901 tggcgcttgc tgttggatta atgcaaggaa tgccgctgga taaagttatt ggctccatca
    75961 aagccggtgt cggcgggacg ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc
    76021 tgggcaaaat gctggcagac tgcggtggcg cacaacgtat cgccaccacg ctgattgcca
    76081 aatttggtaa aaaacacatc cagtgggcgg tggtactgac cggttttacc gttggttttg
    76141 ccctgttcta tgaagtgggc tttgtgctga tgctgccgct ggtgtttacc atcgcggctt
    76201 ctgcgaatat tccactgctg tatgttggtg taccaatggc ggctgcactg tctgtgaccc
    76261 acggcttcct gccaccgcat ccgggtccga ctgcgattgc caccattttc aatgccgata
    76321 tgggtaaaac cctgctgtac ggtactattc tggcaatccc gaccgtgatt ctcgccggtc
    76381 cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat tccggaaggt ctctacagcg
    76441 cgaaaacctt cagcgaagaa gagatgccga gctttggcgt cagcgtctgg acctctctgg
    76501 tgccggtagt actgatggcg atgcgtgcga ttgccgaaat gatcctgccg aaaggtcacg
    76561 ctttcctgcc ggtagcggag ttcctcggtg acccggtaat ggctacgctg attgccgtgc
    76621 tgattgcgat gttcaccttt ggtctgaacc gtggtcgttc aatggatcag attaacgaca
    76681 cgctggtttc ttccatcaaa atcattgcga tgatgctgtt gatcatcggt ggtggcggtg
    76741 cgttcaagca ggtgctggta gacagcggcg tggacaaata cattgcttcc atgatgcacg
    76801 aaaccaacat ttctccgctg ctgatggcct ggtcgattgc tgccgtactg cgtatcgcgc
    76861 tgggttccgc aaccgttgcg gcaatcactg cgggtggtat cgcggcaccg ctgattgcaa
    76921 cgacgggtgt tagcccggag ctgatggtta ttgcggttgg ttccggtagt gtgattttct
    76981 ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta ctttaacctg actatcggcg
    77041 agaccatcaa atcctggtcg atgctggaaa ccattatctc ggtgtgcggt ctggtaggct
    77101 gtctgctgct gaatatggtg atttgataac actgactgcc ggatgcatcg catccggcat
    77161 caaccgcact ctacttcttc ttcgctgcag ctctgcgccg tctgtccaaa tccttcagca
    77221 acttgttcac gccatcatcg gcaaacatcg tctcaagcgt tgcggaaagc ttgcgtcgcc
    77281 agtttttata ctggtaactg gtgccaggaa tattcaccgg ttcggccata tccagccagt
    77341 cttccggctg tagtcctaac agagcactgt tactgtcggc aatgtagcgc tgcaaaccac
    77401 ggttcagcgt cggcgtcatc gacatcaacg atgccttatg cccggcacgt tttggcagac
    77461 aaccatattt atgcagtgca tccagcagcc cttgcttcgc cagttcgcga tcctgataca
    77521 gaccgcgcag taccacttca tccggataca gccccagggt tttgcccaga gttagatccc
    77581 cgctctccca gtaaccacgc agcgttggca ggtcgtgtgt cgccgcaacc gccatcgact
    77641 gctccgggta cgctttcggt gcacggaacg tcttctcgtg gtcgttttcg aaatagagca
    77701 ctttgtagga gtacacaccg ctgctgcgca gcttaccgac aatctctacc ggtacggtac
    77761 cgagatcttc accaatcacc atacagcgat gacgtttact ttcgagtgcc agaatcgaga
    77821 gcagatcatc caccggatag tgaacatacg cgccctgatc tgccgtctcg ccatacggta
    77881 tccaccacaa acgcagcatc gacataacat ggtcaattcg taatgcgccg cagttttgca
    77941 tattggcacg caacagctcg ataaacggtt cataggcacg cgcggtgatg atatgcgggt
    78001 ccattggcgg caatccccag ttctgcccca acgggccgag gatatccggc ggcgcgccaa
    78061 ccgacgcttt caggcaatac agttcacggt cgcaccaggt ttccgcccca ccttccgcta
    78121 cgccaaccgc cagatcacga tacaagccaa tcggcatttc atagccctgg cttatctccc
    78181 agcaggcggc aaactggctg taagccagcc actgcagcca gagataaaaa tcaacgtcat
    78241 cgcgatgttc ttcgcagaac tgacgcactt ctggtgaatc cacgttctga tacatctctg
    78301 gccatgcagg ccagccccag cgcatttcgt cctctttcac ttgctgggca tgtagcgcat
    78361 caaaggctgc ctgccagaac aggctgtcgc cttgatctgc aacaaactgg cgaaacgcgg
    78421 ccatctgctc atcatcacgt tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt
    78481 ttagggcggt aaccgtggag taatcgaccc agtcggcatc ccgcgcctgt tgcagcgtct
    78541 gttgcgtggt cggcaactgc caccaggcct gagcctcttc gctaagatgg aaatcttcga
    78601 cggcgttaac gtcgatataa atcacattca gccagcggcg agaagacggg ctgtatgggc
    78661 tggcgctctc cggatttgcc ggatagagcg catgaatcgg gttcaggccg atgaacgacc
    78721 cgccacgttt tgccacatcc accagcatcg ctttgagatc gccaaaatcc ccaatacccc
    78781 agtttttttc cgatcgcagc gtataaagct ggacgcaggc accccacagc ttttgtttat
    78841 tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac aatcaccctg caatgcgcgc
    78901 gctggtcttc ctgggtaagc gtcagcgtgt gataaccttc cggcagcttc gtcggtagat
    78961 tgaacgcttt gcccccagtt acatggcctt tgtactgcgt tccttcttcg gtggtcagca
    79021 gccagctata ttcgccgctg ccctccacca ccatcggcat ttttttgccg ctggtataaa
    79081 ccatgacatt cgggactggc gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt
    79141 caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt
    79201 aattggggct aatccccgcc gccagcgcgg cattatccag acgtttgctt tccatcgagc
    79261 ttccttagcg ttttgcctgc cagatacgag cctgataatc gcgaatagag cgatccgagc
    79321 taaacatacc gcagcgggcg gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc
    79381 ggtacagcac atccacctgc ttttgtgcct ctacataggc tgcgaaatcc gccatcacca
    79441 gatacggatc gccgccctgt ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat
    79501 cgccgtcgct gtatttaccg ctttccagct ctttcaatac tgcgtccagc accttatctt
    79561 tcttccgcca tttcaccggg tcgtagcctt tggccagaat tgccttcact tgttccacgg
    79621 tatgaccaaa aataaagata ttttcttcac cgactttctc ggcgatttca acgttcgccc
    79681 catccagcgt accgacagta agcgcaccat tgagcgccag tttcatattg ccggtaccgg
    79741 aagcttcttt acctgcagtc gaaatttgtt cggagatatc cgccgccggg atcagttttt
    79801 ccgccgccga aacgcaataa tccggcagga acaccacctt caacttatcg ccaaccagcg
    79861 gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa gataatattc ttcgccaggt
    79921 agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg cggtacgcga tcagcctgcg
    79981 ggttttcacg aatttctttg tacaacgcca gaatatgcag cagattcagg tgctggcgtt
    80041 tgtactcatg cagacgtttg atctgaatat cgaaaatcgc ctgtggattg atctcaatac
    80101 cggtacgaac tttcacaaac tccgccagac ggactttatt cgcctgcttg atctcgcgat
    80161 attgctgacg gaatttcgca tcatcagcga atttttccag attgatcagc tgatcgagat
    80221 cgttagccca ctcttttttc agtgatttat ccaacagagc cgccagtgcc gggttgcact
    80281 gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt atggaatttg ttcggccata
    80341 gctggtgata ttccgggaac agatctttca ccaccagatc cgagtgcagc gccgcaacac
    80401 cgttcaccgc gaaaccgcca accacacaca ggttcgccat atgcacttgt ttgtcgtgca
    80461 ccaccgccag tttggcccac actttttcat cgcccggcca ggttttctcc accagcgttt
    80521 taaagcgagt attaatttcg ttaataatct gcatgtggcg cggcagtaag cctttcacca
    80581 gtttcacatc ccagcgttcc agcgcttctg gcatcagggt atggttggtg taagcgaaag
    80641 ttttgctggt aatggcccag gcgtcatccc agctcatctg gtgctcatcg atcagcacgc
    80701 gcagcagttc tggaatcgcg atagttgggt gggtatcgtt cagctgaata acttcgtaat
    80761 ccgccagttc gtgcagttta cgccccgcca gatgatggcg acgcaaaata tccgctaccg
    80821 aacaggcaca ctggaagtac tgctgcatca agcgcagctt tttaccggca gtatggttgt
    80881 cgtttggata gagaactttg gtcagttttt ccgcattgat gccctgctgt tcggcacgca
    80941 ggaaatcacc gtcgttaaat ttagtcagat caaacggatg cgcgtgcgtc gcctgccaca
    81001 gacgcagcgg ctgcgccacg ccattacgat agccgacaac ggggagatcc cacgcttgac
    81061 cggtaatggt aaactccggc tcccagcgtc cgtctttcgt cactttaccg ccaatcccta
    81121 cctgcacatc cagtgcttcg ttgtggcgga accacgggta gttactgcga tgccagtcat
    81181 ccggcgcttc aacctgtttg ccatcgacaa aagactggcg gaacaaacca tattgatagt
    81241 tcagaccgta acccgtcgca gactgaccga cagttgccat tgaatcgagg aagcacgccg
    81301 ccagacgtcc cagaccaccg ttacccagcg ccgggtcgat ctcttcttcc agcaggtcag
    81361 tcagattgat gtcataagcc ttcaacgaat cctgtacatc ctgataccag ccgagattca
    81421 acaggttgtt gcccgtcaga cgaccaatca aaaactccat tgagatgtag ttaacatgtc
    81481 gctgattcgc caccggcttg gcgaatggct gagcacgcag catttcggcc agtgcttcac
    81541 tcactgccag ccaccactgg cgaggagtca tttcagccgc agaatttaag ccataacgct
    81601 gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag
    81661 gagttccact tttcttagat tttcaacaca acgttatcgc tagtttgcca ggctcgatgt
    81721 tgaccttcct catcctgcgg gggattaggc agggaggagt tgcggggatg agcaaggaaa
    81781 tgtgatctca accacttaaa gctagtgcaa accacaggat tagcagcaaa tcaatgccat
    81841 acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg
    81901 gaaggtaact ctataaatta agtaaaggag tgaaacagtt tcacaagtaa aatatccagt
    81961 gtgctccatc tcattcttaa tagatttatt aagatcatct ttttagatag cactttcatc
    82021 aggaatgaag aagaaaccct tgcttaaatg aatctgatga acataaggga aaccagtatt
    82081 cacgctggat cagcgtcgtt ttaggtgagt tgttaataaa gatttggaat tgtgacacag
    82141 tgcaaattca gacacataaa aaaacgtcat cgcttgcatt agaaaggttt ctggccgacc
    82201 ttataaccat taattacgaa gcgcaaaaaa aataatattt cctcattttc cacagtgaag
    82261 tgattaacta tgctgattcc gtcaaaatta agtcgtccgg ttcgactcga ccataccgtg
    82321 gttcgtgagc gcctgctggc taaactttcc ggcgcgaaca acttccggct ggcgctgatc
    82381 acaagtcctg cgggctacgg taagaccacg ctcatttccc agtgggcggc aggcaaaaac
    82441 gatatcggct ggtactcgct ggacgaaggt gataacctgc aagagcgttt cgccagctat
    82501 ctcattgccg ccgtgcaaca ggcaaccaac ggtcactgtg cgatatgtga gacgatggcg
    82561 caaaaacggc aatatgccag cctgacgtca ctcttcgccc agcttttcat tgagctggcg
    82621 gaatggcata gcccacttta tctggtcatc gatgactatc atctgatcac taatccagtg
    82681 atccacgagt caatgcgctt ctttattcgc catcaaccag aaaatctcac cctggtggtg
    82741 ttgtcacgca accttccgca actgggcatt gccaatctgc gtgttcgtga tcaactgctg
    82801 gaaattggca gtcagcaact ggcatttacc catcaggaag cgaagcagtt ttttgattgc
    82861 cgtctgtcat cgccgattga agccgcagaa agcagtcgga tttgcgatga cgtttccggt
    82921 tgggcgacgg cactacagct aatcgccctc tccgcccggc agaataccca ctcagcccat
    82981 aagtcggcac gccgcctggc gggaatcaat gccagccatc tttcggatta tctggtcgat
    83041 gaggttttgg ataacgtcga tctcgcaacg cgccattttc tgttgaaaag cgccattttg
    83101 cgctcaatga acgatgcact catcacccgt gtgaccggcg aagaaaacgg gcaaatgcgc
    83161 ctcgaagaga ttgagcgtca ggggctgttt ttacagcgga tggatgatac cggcgagtgg
    83221 ttctgctatc acccgctgtt tggtaacttc ttgcgccagc gctgccagtg ggaactggcg
    83281 gcggagctgc cggaaatcca ccgtgccgcc gcagaaagct ggatggccca gggatttccc
    83341 agcgaagcga ttcatcatgc gctggcggca ggcgatgcgc tgatgctgcg cgatattctg
    83401 cttaatcacg cctggagtct gttcaaccat agcgaactgt cgctgctgga agagtccctt
    83461 aaggccctgc cgtgggacag cttgctggaa aatccgcagt tggtgttatt gcaggcgtgg
    83521 ctgatgcaaa gccaacatcg ctacggcgaa gttaacaccc tgctagcccg tgctgaacat
    83581 gaaatcaagg acatcagaga aggcaccatg cacgcagaat ttaacgctct gcgcgcccag
    83641 gtggcgatta acgatggtaa tccggatgaa gcggaacggc tggcaaaact ggcactggaa
    83701 gagctgccgc cgggctggtt ctatagccgc attgtggcaa cctcggtgct gggtgaagtg
    83761 ctgcactgca aaggcgaatt gacccgctca ctggcgctaa tgcagcaaac cgaacagatg
    83821 gcacgccagc acgacgtctg gcactacgcc ttatggagtt taatccagca aagtgaaatt
    83881 ctgtttgccc aagggttcct gcaaaccgcg tgggaaacgc aggaaaaagc gttccagctg
    83941 atcaacgagc agcatctgga acagttgccg atgcatgagt ttctggtgcg catccgagca
    84001 caactgttat gggcctgggc acggctggat gaagccgaag cgtcggcgcg tagcgggatt
    84061 gaagtcttgt cgtcttatca gccacagcaa cagcttcagt gcctggcaat gttgattcaa
    84121 tgctcgctgg cccgtggtga tttagataac gcccgtagcc agctgaaccg tctggaaaac
    84181 ctgctgggga atggcaaata tcacagcgac tggatctcta acgccaacaa agtccgggtg
    84241 atttactggc aaatgaccgg cgataaagcc gccgctgcca actggttgcg tcatacggct
    84301 aaaccggagt ttgcgaacaa ccacttcctg caaggtcaat ggcgcaacat tgcccgtgca
    84361 caaatcttgc tgggcgagtt tgaacctgcg gaaattgtcc tcgaagaact caatgaaaat
    84421 gcccgcagtt tgcggttgat gagcgatctc aaccgtaacc tgttgctgct taatcaactg
    84481 tactggcagg ccggacgtaa aagtgacgcc cagcgtgtgt tgctggacgc attaaaactg
    84541 gcgaatcgca ccggatttat cagtcatttt gtcatcgaag gcgaagcgat ggcgcaacaa
    84601 ctgcgccagc tgattcaact caatacgctg ccggaactgg aacagcatcg cgcacagcgt
    84661 attctgcgag aaatcaatca acatcatcgg cataaattcg cccatttcga tgagaatttc
    84721 gttgaacgtc tgctaaatca tccagaagtg ccagaactga tccgcaccag cccgttgacc
    84781 caacgtgaat ggcaggtcct ggggctgatt tactccggtt acagcaacga acaaatagcg
    84841 ggtgaactgg aagtggcggc aaccaccatc aagacgcata tccgcaatct gtaccaaaaa
    84901 ctcggcgtcg cccaccgtca ggctgccgtg cagcacgcgc agaaactgct gaagatgatg
    84961 ggttatggag tgtgagttta gccggataat gcattggatc cggcttacat ctttatatca
    85021 ttcaatactc acccgcgtta cgccatccgt ttctatcaaa ccaaaccgca ccggcaagaa
    85081 acgctccacc accgcgatat tggtcagcag atggcatgag ggatgggcga ccgtaaattg
    85141 tcccgcgccc gccagcgcca ttggcaacac tagctggtca gcaagatatt cccccactgc
    85201 cgccgggctt gccaggtagc gtttcacctc tttcaccaac tgtgccgcaa ccacctcggc
    85261 actgacgcgc ttttcaccga cgacaaaaaa gcgttcggtg atattttcac tttcgacttc
    85321 aagcgagacg gtattacccg gcccctggtc gcgcggcagg ttatgaatat actgctcatg
    85381 caggggaaaa ctccccgcca gtgtagcgat ttcacgctca gcaacatggc gcggcacacc
    85441 agccagtagc acctctccac gcatctgcac aatgttcccg cgctcgccaa gttgcaaggt
    85501 tttaaacgat gccactggcg agacttctgt tgccaccacg ccgcctccgg cagggtaaaa
    85561 accgtgacgt aacagcgtgg tttgctgatg aattcctatt ttcgccagca gcggctccaa
    85621 cacccggcgg ataaaatccg caggcggggc cgacgggtta tcggtaccgc cgctcacttc
    85681 aacacgcgaa ggtccatcgg caaaccacag cgcaggcagc accgtttgca gcaccagcgt
    85741 acaacttcct gcgctaccga tagcaaagcg gtaatcgccg ccacgcacgg tgccgggccg
    85801 gaagaccaga cgctgcgacc ccagctccgc gccttccacc gttgccctac aaatttccgc
    85861 agctgctttt accgcggtca gatgctggcg caacagtccc ggttttgccc gcccggcacg
    85921 aatgccggtg atggtaaatg gctgaccggt tatcatcgac agactcagcg ccgagcgcag
    85981 gatctgcccg ccaccttcgc cctgtgcgcc atccagcgca atcatccttt tcatcattta
    86041 tccttttacg cacaccacct gacgcagggt atagataact tccaccagat cgctttgtgc
    86101 cgccatcacc gcatcaatat ctttatacgc catcgggatt tcgtcgatca cttcggcatc
    86161 tttacggcat tccacatgcg cggtggcacg aatttgatct tccacgctga acattttttt
    86221 cgctttagta cggctcataa cccgcccggc accgtggctg cacgaacaga acgactcttc
    86281 atttcccagc ccacggacga taaagctttt cgctcccatc gaccctggaa taattccata
    86341 ttgaccagca cgcgcagaca ccgcgccttt acgcgtcacg tagatctctt cgccaaagtg
    86401 ctgttctttt tgcacatagt tgtggtgaca gttgatctct tccattgcca gcgtttgtgg
    86461 ctgtcttacc gttttctgcg taatgctctg caacgccgtt accacgtttt ccatcatcgc
    86521 atcgcggtta aggctggcaa aaagctgcgc ccaggccacc gctttcaggt aatcatcaaa
    86581 gtattccgta ccttccataa agtacgccag atcacgcgac ggcaacgttt caagctggtc
    86641 ctgcatctct ttttgcgcca gatcgataaa ataagttccg atggcgttac caattccgcg
    86701 tgaaccggag tgcagcataa tccacacctg atccgaccca tcaaggcaga tttcaataaa
    86761 gtggttaccc gttcccagcg ttcccaggtg tttatagttg ttagtgttaa ggaaacgcgg
    86821 atatttttgc gttaaccact gataaccggc ctccagctca gcccatttcg catcgacgtt
    86881 aacaggcggg ttttcccatg cgcctttatc acgtttacaa cggccagtgg tacgcccgtg
    86941 cggcaccgca gtttcaatcg cctgacgcag ctccgccagg ttttccggca ggtcagccgc
    87001 cgttaaagcg gtacgtagtg cgttcattcc acaaccgata tccacgccca ccgccgccgg
    87061 aataatcgcc cctttggtcg ggatcacgct accaatggtg gaacctttac ccaggtgtac
    87121 atcaggcatt accgcaatat gtttgaaaat aaacggcatc ttcgccgtat taataagttg
    87181 ctgacgcgca tcggcttcta ccggcacgcc tttggtccac atttttaccg aggcattttc
    87241 agtggtcagt aattcgtaat tcattttgtt tttctctttt cgttggtatg tcctgataat
    87301 tgcaaccgtc gtgccagaaa attaaataag cagattaatt ttttaattca ttgttttaaa
    87361 aaagattata tctttacgtc cgtaaccgga gatttcccgc aaagccgatt taccgataat
    87421 gaaatatcgt cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    87481 ggcaccgtac tggattatgc cggacgcggc agtcagcgct ggtcgaaatg gcgtccgtca
    87541 ctctgtatat gccagcaaga atcgttagtc atcgatcgac tggaattatt gcacgacgcc
    87601 cgctcgcgct cgctgtttga aacgcttaaa cgcgatatcg tcagcgtttc gccagaaaca
    87661 gaagtggtga gcgttgagat tgagctgcat aacccgtggg atttcgaaga ggtttacgcc
    87721 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    87781 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    87841 tacctgcccg cccggttgat acaatcttca cctccgcgca aaaaagaaca gccgcgcggc
    87901 gcaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    87961 tttgccgagg aacgccagca aacgcttgat tttcttaagt ccggtattgc cacgcgtaac
    88021 ccccacttca accgcatgat tgagcagatt gaaaaagtgg cgattaaatc ccgcgcaccg
    88081 atcctgctaa acggcccaac cggtgcgggc aagtcatttc tggcgcgacg tatatttgaa
    88141 ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg aagtgaactg cgcaaccctg
    88201 cgcggcgata ccgccatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    88261 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    88321 attggcgaac tgggcgcaga cgaacaggca atgctgctta aagccattga agaaaaaacc
    88381 ttttatccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    88441 gtgcgtgatt tgcgccagct ggttgccgaa ggaaaatttc gcgaagacct gtacgcgcgg
    88501 atcaatctct ggaccttcac cctgccgggg ctacgccagc gtcaggaaga tattgaacct
    88561 aacctggatt atgaagtgga gcgccacgct tcactcaccg gcgacagcgt gcgttttaac
    88621 accgaagcgc ggcgcgcctg gctggccttt gcgacatctc cccaggcaac atggcgcggt
    88681 aactttcgcg agctttctgc cagcgtcacg cggatggcta cctttgccac tagcggacgc
    88741 atcactctgg aagtggtgga agatgagata aaccgtctgc gctataactg gcaggagagt
    88801 cgcccctccg cgcttacgca gctactgggc gcagaggcag aaaacatcga tctcttcgac
    88861 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcact ttctgcagcc
    88921 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaacga cgcggatcgg
    88981 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag cattgcagga tcagcacagc
    89041 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcatcg
    89101 gtgtagacgg catctaccat gctgatgctg cccatattga ccatcgcgtt acggccaaat
    89161 ttcgagtgat cgacaaccag cataacgtgg cgcgagttct caataatggc gcgtttggtg
    89221 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tatcgatgcc gcttatcccc
    89281 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    89341 ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa tacggaaatc ctcttttacc
    89401 atcagcgtgt tagcaacgtt aagattgttg gtgacaatgc gcaaattgct gtgattaagc
    89461 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccgttg
    89521 gggatttgct ccgccacttt acgggcgatg cgctcttttt cttcggtctg ggtggcttta
    89581 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagtg ccgcaccgcc atgatggcgc
    89641 agaatcaggt tttgctccgc cagctcattg agatcgcggc gaatagtctg cgggctgacg
    89701 gagaagtgct ccaccagctc ctcggtgctg acataaccct gctgtttaac cagttcgata
    89761 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    89821 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaacccgg cgatgtgtgc
    89881 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    89941 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    90001 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    90061 gaatttttgc tgcacatagc cgcttaacag ggcgctaatg agagtaataa caattagctt
    90121 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaga gcaggttaaa
    90181 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    90241 tttcagtgct ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    90301 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtta ccggacctgc
    90361 gcgttcacgc aaggcggcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    90421 gcctgcctgc cagctcgccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    90481 cagctccgcc cgtacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    90541 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    90601 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    90661 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgacgttgc
    90721 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    90781 tgcgccgcgc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    90841 tcgttattac gcataaaagc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    90901 tgtcccattg cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    90961 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    91021 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    91081 taatgttata catatcactc taaaatgttt tttcaatgtt acctaaagcg ctattctttg
    91141 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    91201 aaaccaaaga tctgattgtg atagggggcg gcatcaatgg tgctggtatc gcggcagacg
    91261 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    91321 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    91381 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggcc ccgcatatcg
    91441 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct acgcccggcg tggatgattc
    91501 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    91561 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    91621 ccgactgttg ggtagacgat gcccgtctgg tgctcgccaa cgcccagatg gtagtgcgta
    91681 aaggcggcga agtgcttact cgtactcgtg ctacttctgc tcgtcgcgaa aacggcctgt
    91741 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    91801 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgacgggatg catctgcctt
    91861 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    91921 aaaagcaggc ctacattctg caaaacgaag ataaacgtat tgtgttcgtg atcccgtgga
    91981 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggcgat ccgaaagcgg
    92041 tgaagattga agagagtgaa atcaattacc tgctgaatgt gtataacgcg cactttaaaa
    92101 agcagttaag ccgtgacgat atcgtctgga cctactccgg tgtgcgacca ctatgtgatg
    92161 atgagtccga ctcgccgcag gctattaccc gtgattacac ccttgatatt catgatgaaa
    92221 atggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    92281 cggaacatgc gctggaaaaa ctaacgccgt attatcaggg tattggcccg gcatggacga
    92341 aagagagcgt gctaccgggt ggcgccatcg agggcgaccg cgacgattat gccgctcgct
    92401 tacgccgccg ttatccgttt ctgaccgaat cgctggcacg tcatttcgcg cgtacttatg
    92461 gcagcaacag cgagctgctg ctcggcaatg cgggagcggt aagcgatctt ggggaagatt
    92521 tcggtcatga gttctacgaa gcggagctga aatatctggt cgaccacgag tgggtccgcc
    92581 gcgccgacga tgccctgtgg cgtcgcacaa aacaaggtat gtggctaagt gcggaacaac
    92641 aatctcgtgt gagtcagtgg ttggtggagt atacgcagca gaagctgtcg ctggcgtcgt
    92701 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaacaca
    92761 aaattgcctg atgcgctacg cttatcaggc ctacgtggtt tatgcaatat attgaatttg
    92821 catgattttg taggcctgag aaggcattca cgccgcatca ggcatgaaca aagtgccacg
    92881 ttgtcaacaa tctgacctac tttttgattc agggaattac cgcaaagccc acgccatcat
    92941 acccaacgta acaaaactta agcacgttcc ccactcactc cacttccgta tctggcatct
    93001 atttttgctg cgccactggc aataatatcc caacgcaaaa ccaagctgtg cgcttattgg
    93061 accgataaaa tccgtccacg gctgaggcca aaatggcgaa aaattcaggg gtagccagac
    93121 aacaagcaga aagaaattgc ggatactttc cagcaacgca attttcgctg gcgcgatgta
    93181 aaaaatgata ttccagagga tcaaccataa atagaccggt gcgaatacca accaacaatg
    93241 gagcctgctt ttcaattcag attgagttct acgagtaaat tgccaaccaa cgcaacacat
    93301 tacacagaag aaaagcgcat attcagccga taaccaaagt tgtaattcca gaagggaaat
    93361 acgcctgtgc atgaacgaat ttgagaacag catctccatt agaaaactca gtgtgatacc
    93421 aacaacaacc accgtcggcc acaccaacaa cggcacaata acaaagccga gatatagcgg
    93481 ataaatataa agtctgattt tattcatctt tattgtcctg attattcggg tgatgcgtca
    93541 tacatcgcgc ataaacactc atcaatcatc cagtcgtctt cccagacttg atattctgtc
    93601 attggttcag gctgttttag tgggttctca atcgcttgtc gcgctctttc tttcagcaaa
    93661 ggatgattat ttctagccca ggtattaatt tgagtggtaa tagttttatt atcatcactc
    93721 tgctcaaaat cgctattttc gacgtattct ttactgttac gccagagcaa acatgccata
    93781 gacgccgtcg gccagtaacc ggaaatggac ataatggcgt caagtaaggg ttcaatcttt
    93841 ataccaccgc gataaaacat ttctaatatt atttccagtt ccacgacctc cttagccgac
    93901 acccaatcct gcatatataa ataggcgaaa tcgctaatta tttgtagttc atccgttcgc
    93961 cagttctggt tatctgcctc tgctattctc tgtaaacagc agccttcata aatgcctgga
    94021 aattgccttt gggcaataaa ttcaaacaat ctgggtaata aatagcgaat ctcatcatca
    94081 ttttgcgggg taggaccaat acttgttccc caggcatgta ataaagggaa aggtatggca
    94141 cgcagtgaaa cccctcttaa ggcttgttgt tgttcttcgc taaagcagta aggacaacaa
    94201 acatgaaatc tcgacggcac cggataacga cgaaaaatat gataaatctg ctcaattaat
    94261 gcctgcatgg cggggctgca ttgcataatt actccctctt ccgctgattg cgtgtaaacg
    94321 gttgataaac aaaacgagca ttggccgcgt tcaggcgaat aataccggta tccatgtgat
    94381 tatcctgctt aaggtaaggt tttatacatc ggcacataac ctacatcttc caccaaactc
    94441 tgcccctgcg gcgttaaaaa ccattccacc aatttttgtg tctctgacgt cgtattttcc
    94501 ctcgttacca taaaggcatc gatgatatag ggatatttgc cattgcgaat attttccgca
    94561 gtcggtgcaa taccgttaat cgctagcaat tttatatttt tatcagcatt catttgcgtc
    94621 gcgtaatagc ggaaggtata gcctattgcg ttgtttgtat tacggtattc ggcaacgact
    94681 ttaatcattc cctccatcac gcttgccact tccgtttcct gcggcgagat catgcggaca
    94741 tttttcatga cctgtgattg catcactgtc tggctgccag agtcttccgg gcgctgccag
    94801 gtctggatct cctgatcgtt accgccaaca gtacgccagt tggtaattgc accactgaaa
    94861 atgtcacgca cctgttgttc agtcatggaa ttaaccggat tatccgcatt gacgatgaaa
    94921 acgaatgctt cacgggcaaa gggggtgtac agcaaagtga caccagattc ctttgcacgt
    94981 tttttttgcc caccggaagg ttgcgccacg aaaataatat cagcatcgcc cttaacgatt
    95041 ttgttatatg cttctggcgt acgagagttt tccagataat cccaaacgtg aaaatcctct
    95101 ggtattacac ttaatgcata aaatgcagaa gcataaattg ggtacgcagc cgtggcgcca
    95161 tcgattcgcg gccagttttg cctaaactga atttgcggtt tgccacgcag cggcgtcagt
    95221 cgattattga gtttatcggg ttgccaggcc cagatatcaa tattttcgcg tacaccgaca
    95281 ccaggattga gctgtttata taaataaccc tgataaccag cgacaaaacc aagcatgaga
    95341 aggataaaca ggagggaatt acggcaacgt agcccggatg taccggttaa aggatgtctg
    95401 gttggccggc cgtaccccag cgaaaaactg atatgcgcgc caacgggtat aatgactatc
    95461 caccagtact cacctccgac tactgagact aacaaaacgc ccagccaggg gagcataaga
    95521 tcagccaacc aacccgatcc catctggtga aattgataat ctgccagatg cagcgcgatt
    95581 atccagccaa ccagggttaa tagcgcgggt aagagaaaaa agcattgccg ccagacggct
    95641 atttctctgt tttctggcgc gctatcaccg gcccacacgc gaccagagaa aaatattatt
    95701 atcgccccca gcgtcagcat aaagacgttg agtagaagat aaattgttgg cgtgataagg
    95761 atattacaaa cttcaataat accggtaagc ccgacaccca gcatggcaac aaccgctgcc
    95821 aaaaattgcg ccagtatggg gatgccgaaa aaagtcatta tcagcgaggt caaaatccat
    95881 tttctatttg gcatcattct gtccattctt cctgaatgat ggaattatac tccccgagaa
    95941 ccctcgcccc ctctggacac ttttccgaaa tgatggcgaa aaaaacggga ccctttggcc
    96001 ccgttctatt tattggtgaa attacaatct caccggatcg atatgccaga tatgatcagc
    96061 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
    96121 ttttgcggtc cactcttcct gaagctcgta aagttcgtca actttatcct gacaatcgac
    96181 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcaac
    96241 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
    96301 cacctgatgc agctcctcat ctttctcata gtattcacgc ggtttgtagc cctgacgacg
    96361 cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag caccgacatg
    96421 atccagcatc tcgacgtttg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc
    96481 aaacttcatg ttactggtgc cggaagcttc cgtccccgcc agcgaaatct gttcagacag
    96541 atcggccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
    96601 tttcagcttg tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
    96661 atggataata tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
    96721 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
    96781 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
    96841 cgccttagga ttcaccacca cattcagctg ctgggcgata tactccgcca gacgcttttt
    96901 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
    96961 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
    97021 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
    97081 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
    97141 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
    97201 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
    97261 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
    97321 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
    97381 gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
    97441 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
    97501 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
    97561 ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg
    97621 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
    97681 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga atttaccgag
    97741 gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg cgtcggtgtc
    97801 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
    97861 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
    97921 gcgtttgaat tcccacgggt taccatattc cagccagtag tcaggcgact ctttctggct
    97981 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
    98041 cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacgtc ccaggccacc
    98101 gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
    98161 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
    98221 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
    98281 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
    98341 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
    98401 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
    98461 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
    98521 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
    98581 cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc
    98641 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
    98701 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
    98761 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
    98821 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacttg
    98881 gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
    98941 gagtagcgcc agctgcccgc cctgctccag aaggcccggt aaggcttcca gcaccaggtc
    99001 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
    99061 cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc
    99121 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
    99181 gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
    99241 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga ccgccgtaat
    99301 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
    99361 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
    99421 gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc
    99481 cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca
    99541 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
    99601 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
    99661 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
    99721 gatatgcccg gcaaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg
    99781 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
    99841 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
    99901 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
    99961 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
   100021 ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc
   100081 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
   100141 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
   100201 acacaaccgc cggaaaccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
   100261 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
   100321 atatccagct ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc
   100381 gtacccacat cgcgccagta cggctcggca tccggatcgg attgtacgca agagagcggg
   100441 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
   100501 tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag atagtcggcg
   100561 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
   100621 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
   100681 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
   100741 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
   100801 aggatcacca cgtattccgc tttataacgg cgaataatat cgaggttttg ggtgaccgca
   100861 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
   100921 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
   100981 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta
   101041 gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
   101101 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
   101161 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
   101221 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag
   101281 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
   101341 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
   101401 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
   101461 atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccaccaaag
   101521 cgggaatacg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
   101581 cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat
   101641 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg gagagcaaca
   101701 acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccagatca agagtaccgc
   101761 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
   101821 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
   101881 catgtgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
   101941 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgcagccaga
   102001 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
   102061 ccacctgata accaccagga gcgatatccc acggttcagc aattaacttc acctgcgaga
   102121 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
   102181 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
   102241 aacgcaggca ggcgctggca taatccacca ccgccgggtg actcaaattg agcgtgttgc
   102301 cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttgt
   102361 cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
   102421 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
   102481 catgcagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
   102541 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
   102601 gcgccactgg cagcagttcc agcgcggtaa tgcccaactg tttcaaatag ttgatcatca
   102661 ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaggt
   102721 acgttaatcc tttaacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg
   102781 gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
   102841 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact
   102901 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
   102961 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
   103021 ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct
   103081 ggccattggt gtcaaagaca cacagctcta cccgctcggc atgagcggag aaaagtgtga
   103141 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
   103201 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg tcaggcttag
   103261 tgaatgctga cgaccatggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
   103321 attactgccg tgatagtgca tggaatcggt attgaggatc tcacgccatt tgcctggctg
   103381 attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg caacgatgat
   103441 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
   103501 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcatcgctt tatggtggcg
   103561 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
   103621 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
   103681 gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat agtaagcgcg
   103741 caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
   103801 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
   103861 cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct tcatgtagtc
   103921 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
   103981 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
   104041 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
   104101 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cacgataaat
   104161 catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
   104221 cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaaa tcagcgtgtt
   104281 ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg taccatcaaa
   104341 ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga gaatcacgtt
   104401 cagaccagct gcgtgtgcag catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
   104461 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
   104521 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
   104581 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggagcc
   104641 caggtgaact tcatatatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
   104701 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
   104761 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
   104821 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
   104881 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
   104941 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
   105001 atctgcatgc gcgcctaaag tttcatacgg gcgcaggtga gtaccttcag ataatagcca
   105061 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
   105121 cccatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
   105181 actaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
   105241 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
   105301 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
   105361 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
   105421 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
   105481 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattcc
   105541 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
   105601 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
   105661 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
   105721 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
   105781 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
   105841 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttccacggtc
   105901 ggaatcgaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
   105961 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
   106021 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
   106081 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
   106141 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
   106201 tcatctgcta catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
   106261 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
   106321 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
   106381 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaata
   106441 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accctgccat
   106501 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
   106561 atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt
   106621 ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac agggcgaatg
   106681 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
   106741 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
   106801 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
   106861 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
   106921 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
   106981 agttttattg atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact
   107041 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
   107101 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgag
   107161 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaattgcca ttaagatgat
   107221 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
   107281 gccgctggca attccgctgg tcgccgggcc aactattctc gccacgctga tgttgttgtc
   107341 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
   107401 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
   107461 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
   107521 cgacggcatt cgaatgtgga tgaaggggta aggttcaaac aggtgctgca cacaaccaga
   107581 cgcggcacct gttttattac ctgaactgat aatgaccttc gcccaatcgg ataacccttc
   107641 cctcttcttc tgcgcggttc agccacacac taatttgttc gatggttaaa ccagtacaag
   107701 acgccagcct ttcaatactt atcgattcct ttgcaaggat cgccagttcc gccacaaaga
   107761 gttgataaaa atcgacaggc gtatacgcgg gcattactgc gtaatttgcg tcatcttcat
   107821 gcagcgaaaa cagttctggt tgagcgttac ggagtgctct gtcatacatc agcccaggaa
   107881 cgtctggcag tctgatcgtc acattctcag cctgttcagc actccatgac gccccatttt
   107941 ctactaatcg tgaattggct gattgcatat cctgatttgg tttaacctgc acaggcagcc
   108001 actgatgttt taatgcctcc atcgccccgg ttatcgttcc accggtcatt cccgcacgta
   108061 caaccattgc gctttcagca aggcaataaa tatatttatt tcgcgccatc gcattgccga
   108121 cggtgaaacg tacctctggg taaaacggcg aaatcaacac caggttgcct gctataagcc
   108181 cttcacgcca tttcactaac gtactcgttt ttaacaagct atcagctaat acgccaacag
   108241 cagttccccc ggcctccagt gccgacgcca ttgcacattc atcaatacct cgcgcaccac
   108301 cagagataac gcaaatcccc tgttgggcca gtttcgcggc cagttgttgg gtatagcgca
   108361 aatcgtcagt cggagcatct cgcgagccaa caatcgccat accttccgct tgcagtaatg
   108421 ctttattccc gcagccaaat aaaacgggag gtgcatccgt tcgcaatcgg tttttcaagc
   108481 gaacgggata atcagcatct ccgcgggtta agatccataa accggcgcgt tgccacttat
   108541 ctaccgccaa cgccagacta tgaccacgcg ccagcaagcc aagaatacgc tcctgagaaa
   108601 tacgcggatc gctccagcct gtaagtttct cttgcggttg cggcaccaga agctcggcgg
   108661 gactgatacg ttggtgcttc agccataatg caaacttccc ccattcacta ttactaagag
   108721 gtttatactc actcgccgcc gcgcgagaaa aatcgctggt tagcaggaga gttgcttgtg
   108781 cattggctga aagattcatc aattttttac cgaggtagaa gcaagggcaa gaggataaac
   108841 cgtcgggcaa cctgcctggc gtaacagtgc tgtcagaact gtcagcgtcc atgcagaatc
   108901 cacgatatcg tcaaccagca acgccggacc aggcatcaaa ggaggggtaa tcacaaacgc
   108961 cccgtcgaga ttttgacact ggtggaaacg gttttgctgc attttctgcg gtggattgtc
   109021 cacgactttt tcaatggcat caataaaagg taagccaagt ttcgccgcca gtcgccgggc
   109081 aaaatcagga accaggtcga ggtgctttaa tgaaggaacg cagcataccc aggttggttc
   109141 aggatgcgga ttccagcgtt ggcgaaccat ttccgcacat gcctccacca gttcatcact
   109201 gaatcgcccc gtttttttct cctgtgctac ctgctttccc cacccggaat ctccccaacg
   109261 agaaaggatt cgccccgtag atccttgtaa actcgcaggc aagttccctt taaatccgta
   109321 ttgcgtaaaa gccccagctg ccacctgttt attgagattt aatggcaggt cagcgtgttt
   109381 aataaataag tttgccgcat ggagtaagcc gctatcaatg tcgggcgaca acaacggatg
   109441 ctgaagacaa ctgctacatt taccacaatg ttcggcacct ggctcatcga gtgcgcggcg
   109501 cagaaattgc attttgcact ccttggtcgt gatatagctt tcaacgtctg ccagctcact
   109561 ttttctctga ttcatcaaat ggttaattcg ttcatggtca ggagaaaaac tgacaattgt
   109621 tctgcgccat aatttctcgg tatacaccac aggcgatgga ttttcagcta ccaataattt
   109681 cagtgctttt tctatttgcc cataacggag attcgtccgt tgttcaatgc ctcgtaaggt
   109741 aagaccgtca ttcacgctaa gtacgttgag tatttcatga atttgcgcct ccgcaggaaa
   109801 ggcgctttca cggaagaatt tatgaatagc acggtcttca ctgccgcaaa gcagtatgcc
   109861 aacagcagaa tcgataccgc gaccggcacg ccccacctgt tggtagtaac caactatcga
   109921 acctggcatc tggtaatgaa taacaaaccc caaatccggt ttgtcaaagc ccattcccag
   109981 cgccgttgtc gcaaccagca ctttaatttt atttgccagc aacgcctgct ccagatattc
   110041 cctggcagta ttagaatctt ccgcgccttc gcaagtcacg ccgctgtagt aagcaaatgc
   110101 actgatacca tttttcctta accactcggc gacaagttcg gcatcgcgag tagtcagggt
   110161 ataaacaatt cccgatttgg aaaactgagg gataaccgtt gctaaccatg ccagacggga
   110221 tgactgttca cctaagacta aggcatcaag cgccaaactt tcgcgagcca gcgttccacg
   110281 ctgaatcaca atgtcaccca attgctgacg gatatcctca acgacacggt tattcgctgt
   110341 cgcggttgta cccagaacag gggtattcgc aggtagttgg cgcaaaatat ctaatatacg
   110401 tcggtaatcc gggcgaaaat cgtggcccca atcagagatg cagtgcgcct cgtcgaccac
   110461 aagcaagcca atacgatcgg ctataggata taaaaccgtt tcgataaagt cctggtttgc
   110521 caaacgttcc ggggaaacca gcaagcaatc gacctcacct tgcagcaact tatcgctaat
   110581 gcgctgccat tcttctctgt ttgtagaatt caacgtttca gccgtaatac ctaaccgttc
   110641 agcggcggca acctggttac gcatcagggc aagcaaagga gagatgataa ttgtcgggcc
   110701 agcgccgcga tcgcggaaga ttttactggc gatgaagtac acagcacttt tcccccagcc
   110761 agtgcgttgg acaaccagca gttttcgccg ttggttcacc accgcgtcta tggcttccca
   110821 ttgcccttcc ctgaaagtag cggtggtatt tcctaacatc cgctgtaaaa gcaattcagc
   110881 tgcgtgcttt tccatcttta tctcctgcga tctttaggca gtgattagaa cggattattt
   110941 gcaggtaaat ttgcgagaga gatcaaaaag atgaagagag gagaaaagag aaatgaatta
   111001 ctgcgagcaa gctcgcagta atttgtcgca gtaattaact caacagctgg aacgcaatca
   111061 tcccaacgat ggcaccgaca gtgccgagga tggtttccat catggtccag gttttcagcg
   111121 tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaaccagcg tcgttaacgt
   111181 ggctgacaac aatcgaacca ccggcgatac aaatcgacag cgccgccatt tgcgcaccgg
   111241 agtagttcag ttgttcaata accggcatca ccagtcccac cgccgttaaa caggcaacgg
   111301 ttgcagaacc ctgaatgatg cgcactgcag cggccagcac gaagcaggtg atggcaatcg
   111361 gcaggcccat gccggttaac gcttcaccca gcgccggacc tacgccagag tcaaccagca
   111421 cctgtttgaa cacgccgcct gcaccaatca ccagcagaat gatccccgcc ggttgcagcg
   111481 cgtgaccgca aatctccatc actttgtctt ttggcatgcc ctgacgcatt gccaggccat
   111541 aaatcgctac cagacaagca accagaatcg cggtaaacgg atgaccaata aactcgaacc
   111601 attcgtaagc ggtagagcct tccggcacaa aacgcgcggc aatggttttc agccccacca
   111661 gcaccagcgg cagcaggatc agcgacaggc tgaatccgaa ggacggcatt ttgccttcgc
   111721 cgagatgcgg ctcgctgatg tcgtcaggaa tatgcagctc aacgtaacgg ctgatgaagt
   111781 taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg ccaatcagga
   111841 tcatccagcc aaaatcggcg ttcatctgcg atgccagcag cattggcgct ggccctggca
   111901 ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg gattaccagc ttcaccaggt
   111961 tcgtaccggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca atcgccactt
   112021 caaagaacag cggtagcgca cagaccagcc ccgcaaggcc gatggcataa tgcgcgcggc
   112081 tgtgaccgaa ggatttaagc attttgacgg caatctgatc gactgcgccg gtttcatgta
   112141 agatcttgcc aaacatagct cccagggcga caaccaccgc caggaagccg agggtgcctc
   112201 ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa aaaaggccag
   112261 cccccatgga caccaccatt aaagccagga aagcgtgcat acgcgccttc atgactaaaa
   112321 acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta gtcactactt
   112381 atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc ggttgatcga
   112441 tatccaccac cagtacatcg gtttcgtccg cacccggctc ctgcagcgtt tcaaactgcg
   112501 tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg ctttcaatca
   112561 catcaaaatc gcctttcagg tagatgaaag agagattcgg attaccttca cgcagcaagt
   112621 cgcgatagtg ttttttcaat gcagaacaga cgatcagcga cactttatta gtgcgctgca
   112681 tagcaaacgc ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg tcattcagcg
   112741 gttcgccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg ccatcaagaa
   112801 acgcggcatg aagttgatgc gccacttcac ttgcgaccgc agatttgccg ctgcccgata
   112861 cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg gtgcccccac
   112921 aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattctcc ggccggacaa
   112981 tatcagaagc aatatccatc cgtgccttaa gtgtataagt gtgagctact tcaaatttgt
   113041 gggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt ttcggtgtca
   113101 cagattcgcc acgaatacgc gccagaaggc gttcagcgcc aatactgccc atccgctcac
   113161 gcggcgtcag cacgctcgca agtcgtggct ccatcacctg accaatgtca tgaccgtgga
   113221 aaccggcaat cgccatatcg tcaggaactt ttaaccccag acgctgacat tcaaacgccg
   113281 cgccgaccgc cagatcatca ttagtacaga acacgccatc cagctgcgga tattcccgcc
   113341 gcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca accatcacgc
   113401 tatacggcac caggcctgca tcccgcatcg cctgttcgta tcccttctgt ttgatgatag
   113461 tacgttcgtc gagacgtgcg ccgagatagg caatatggcg atgcccgcga gcaataatgg
   113521 cggcggtcat ctggcgtgcc gcttcaaagt tatcaaaacc gacggcaata tcgaggcatg
   113581 gcgacttgct gtccatcagt tccaccacgg gaatccccgc cacttcaatc atctttaagg
   113641 tgcgcggcgt gtgggtacgt tcggtgagga taagtccgtc gatattccat gagagcattg
   113701 attcgagacg ttcttgctcc atttccggtt tataaccgta gtgggccagc atggtctgat
   113761 aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa acctggttgg
   113821 tgagagaagg taacaggacg ccaatcgccc ggctggtggc gttagagagg atatcgggcg
   113881 cacgattggg aatatagccc agttcatcaa gagccgcggc aatcttgccg cgtagagcga
   113941 cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt cattttggtc acgcctacac
   114001 ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag gtacaaaaga
   114061 gatagattct cttagtttaa cacggacgca tcagaacctt cccggggaaa acagtgcttg
   114121 tttacaatta ttagatatac actaaatcaa ttgcatcgca ttgtgctaac gacgcggcaa
   114181 aaccccggaa gcgtacatca gcacgcgacc ggagcgagcg aggaagcctg caaaagggca
   114241 ggcttcaaag atgccgttga gttttaaacc ggcggcagat cgaacagtaa cacttcgctg
   114301 tcgctatccg catggatgga gattgcctgc tcatcccaga ttgccagacc atcgctggtc
   114361 gaggctttca cgccgttaat ggtgacattg cctttcacca cctggatcca gacgcggcgt
   114421 tcagcggcaa tctgatgcac cgactgctca tctttcagca acgcccagcg gtacagttcc
   114481 atatcctgat gcactttcaa tgaaccatct cgcgcatctg gcgaaagcac cagttgtttg
   114541 ccctgcacgg catcgaagcg acgctgttca taacgcggcg taataccgtt ttcttcaggc
   114601 atgatccaga tctgatacag atgcagacgc tcggtgctgc ttgggttgta ctctgagtga
   114661 cgaatacccg tacccgcact cataatctgg aactcgcccg ccggaacctg ctctttattg
   114721 cccatgctgt cctggtgctc aacagtacct tccagcacgt aggtcaaaat ttccatatct
   114781 ttatgcgggt gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt aatcacgcgc
   114841 agcgcggaga agcccataaa gttcggatcg taatagttgg caaaagagaa agtatgccag
   114901 gagtccagcc agccatgatt tgcatgacca cgttcatttg ctttgcgtaa gtagatcatt
   114961 tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg acccgatctg cctgggatga
   115021 tagagggtga aaattgaccc ctctgttcaa aaaaattgaa ctatttgagg ggccatcaaa
   115081 cttacttcgc gagagtcacg gtggagggag aggcttgctc gaatccgcgt tcaaggattt
   115141 ccagattcgt aagaacttca gattccttga cgtaatttgg cgcaccgtgg gtgatggttt
   115201 gatacaacgc atcataaacg cgcccgtaat cgcccatctc cggcttcatc tcttctctga
   115261 ccgtcacgcc ctcgtcattg acatactcca gcacaccgac cgaatcatcc gctgcgaatc
   115321 ccggttcgcc cggcataata ttagccttca ggctggtttc ctgctggtcg ataccgtatt
   115381 taataaacga acctttctta ccgtgaacga taaatttcgg ataatcgatt ttcaccagat
   115441 ggctggtttt gacgatggct tttagatcgc cataaaacag ttgcgcttcg aaagtatcgt
   115501 caggattggc tttattgcgc aggctgcgga tgtcataagc gacgtgatcc gggcgaccga
   115561 acagagaaat aatctggtcc atcgtatgca caccaaggcc atagaacgcg ccatcctgcg
   115621 gcagcccagg tttggtttct gccaccgggc ggtaatagtc aaaatggctt tccacttcga
   115681 caatctctcc cagcttgccg ctttcaatcg cttttttcgc tgtcaggaag caggaatcaa
   115741 agcgacgatt ctgatatggc gtgacggtca gccctttgct tttcgccagt gcaaacagct
   115801 ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac cagcacattt ttcccggctt
   115861 ccagcgcgcg tttcgcgtac tcgaaatggc tgtccgcgtg ggtgcagacc acaaccagct
   115921 taacatcggg atcgtttagc acttcgtcga gatcgctggt gaaatggata tgagagtaaa
   115981 tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat atgcgcgaca tgccagctat
   116041 ccttgcggtt aagtacatac ggcagatggt aacgggtggt gcttttgccg aagccaataa
   116101 aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg gttatttaca ccttagcgca
   116161 aagcggacgt ggttcctact ggagtgcgca taacgaacac aagcacttcc cgaggataaa
   116221 ttgagaacga aagatcaaaa aaaaagccag cacccggctg gctaagtaat actggaagca
   116281 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
   116341 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaaatgca tttgactcgc
   116401 attcgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
   116461 agatagtgat acgccatgct gaaacgcgcg attacgaggc catcaggcag attcacgccc
   116521 agccggaggt gtattgcaac acattacagg tgcctcatcc ttccgatcat atgtggcagg
   116581 agcgactcgc cgatcgtccc ggcatcaaac agttagttgc ctgtattgat ggaaccgtcg
   116641 tgggtcacct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
   116701 gtatttgtgt cgactcccgt tggaagaatc gcggcgtcgc cagcgccctt atgcgagaga
   116761 tgattgaaat gtgcgacaac tggttgcggg tagatcgtat cgagctaacg gtgttcgtcg
   116821 ataacgcgcc agcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta
   116881 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaagt
   116941 aaagaatatt ctcaaattgc ctgttaccaa tgtgaataat tagttttgtt catctaattt
   117001 gagtttttcc ttatattacc accatcattt tgaaataatt tgtaacatca agagactttt
   117061 gaatcattac tcgttaatat ttcttattaa agagaacata ttttcgcaag caatagttat
   117121 tactgtttgt ttatggatag agagtctact taatgggtac agcagctata agtcatttac
   117181 gatatgatct taacaaatat gccctttctt tacgcaaaac cgcaacactt gcttcaacat
   117241 tttttattga gtccccatta gttagattcg agtatctgca agaaatagaa aatactatta
   117301 atgatattac tcacagattc aattcttcat atgacatcaa tgaaaaaaca cgacttatca
   117361 atgaattaaa aatggagtca gaaacagcaa gaaaagaata tcaacttttc agacaaggaa
   117421 attatgataa atatatcact actgatattt tcgaagaaca tggtttgata aaatatgtga
   117481 acttaagttt cgatattgtt gcatctgtag gacaggtcgt aggcggggtt ggcgcgttga
   117541 aatttggtaa ggtagtgcat tcaaaccgaa taaaaggtat aggcgtcaca ttagttgctc
   117601 atggggcgaa taatttttat gaaagcttat cacctttatt ttttaacgag tatgattcag
   117661 gtccaatacg tgaactctac agagtaatag caaaaaaaat gggaggtgat attaattctg
   117721 ctgattatgc atacagcatc gtggactttt cgattactgc atatggtgga tattcaggca
   117781 tgaaaatcgt acctaagtat aatagattga ttcgtccttc attaggaaat agacctggta
   117841 caggacgatt atttcattac acatcagttg attttaaaaa taaattctca tt
//
LOCUS       DAZGCQ010000002       100264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000002 DAZGCQ010000000
VERSION     DAZGCQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.002"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..705)
                     /gene="gmhB"
                     /locus_tag="ACHK07_000107"
     CDS             complement(130..705)
                     /gene="gmhB"
                     /locus_tag="ACHK07_000107"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO8100506.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFINGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            893..1924
                     /gene="metN"
                     /locus_tag="ACHK07_000108"
     CDS             893..1924
                     /gene="metN"
                     /locus_tag="ACHK07_000108"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO8100507.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1917..2570
                     /gene="metI"
                     /locus_tag="ACHK07_000109"
     CDS             1917..2570
                     /gene="metI"
                     /locus_tag="ACHK07_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO8100508.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2610..3425
                     /gene="metQ"
                     /locus_tag="ACHK07_000110"
     CDS             2610..3425
                     /gene="metQ"
                     /locus_tag="ACHK07_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO8100509.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3543..3947
                     /gene="rcsF"
                     /locus_tag="ACHK07_000111"
     CDS             3543..3947
                     /gene="rcsF"
                     /locus_tag="ACHK07_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO8100510.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            3944..4651
                     /gene="tsaA"
                     /locus_tag="ACHK07_000112"
     CDS             3944..4651
                     /gene="tsaA"
                     /locus_tag="ACHK07_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO8100511.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4762..6480
                     /gene="proS"
                     /locus_tag="ACHK07_000113"
     CDS             4762..6480
                     /gene="proS"
                     /locus_tag="ACHK07_000113"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO8100512.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6533..7357
                     /gene="yaeF"
                     /locus_tag="ACHK07_000114"
     CDS             6533..7357
                     /gene="yaeF"
                     /locus_tag="ACHK07_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO8100513.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQAIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQICSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7557..8267)
                     /gene="nlpE"
                     /locus_tag="ACHK07_000115"
                     /gene_synonym="cutF"
     CDS             complement(7557..8267)
                     /gene="nlpE"
                     /locus_tag="ACHK07_000115"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO8100514.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQVAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8281..8703)
                     /gene="arfB"
                     /locus_tag="ACHK07_000116"
     CDS             complement(8281..8703)
                     /gene="arfB"
                     /locus_tag="ACHK07_000116"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO8100515.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(8700..9033)
                     /locus_tag="ACHK07_000117"
                     /pseudo
     CDS             complement(8700..9033)
                     /locus_tag="ACHK07_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
     gene            9199..9399
                     /gene="yaeP"
                     /locus_tag="ACHK07_000118"
     CDS             9199..9399
                     /gene="yaeP"
                     /locus_tag="ACHK07_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO8100516.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9386..9646
                     /gene="rof"
                     /locus_tag="ACHK07_000119"
     CDS             9386..9646
                     /gene="rof"
                     /locus_tag="ACHK07_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO8100517.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9695..10993)
                     /gene="tilS"
                     /locus_tag="ACHK07_000120"
     CDS             complement(9695..10993)
                     /gene="tilS"
                     /locus_tag="ACHK07_000120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO8100518.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(11058..11447)
                     /gene="yaeR"
                     /locus_tag="ACHK07_000121"
     CDS             complement(11058..11447)
                     /gene="yaeR"
                     /locus_tag="ACHK07_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8100519.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11504..13645)
                     /gene="ldcC"
                     /locus_tag="ACHK07_000122"
     CDS             complement(11504..13645)
                     /gene="ldcC"
                     /locus_tag="ACHK07_000122"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO8100520.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13744..14703)
                     /gene="accA"
                     /locus_tag="ACHK07_000123"
     CDS             complement(13744..14703)
                     /gene="accA"
                     /locus_tag="ACHK07_000123"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO8100521.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14716..18198)
                     /gene="dnaE"
                     /locus_tag="ACHK07_000124"
     CDS             complement(14716..18198)
                     /gene="dnaE"
                     /locus_tag="ACHK07_000124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO8100522.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18235..18831)
                     /gene="rnhB"
                     /locus_tag="ACHK07_000125"
     CDS             complement(18235..18831)
                     /gene="rnhB"
                     /locus_tag="ACHK07_000125"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO8100523.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            complement(18828..19976)
                     /gene="lpxB"
                     /locus_tag="ACHK07_000126"
     CDS             complement(18828..19976)
                     /gene="lpxB"
                     /locus_tag="ACHK07_000126"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO8100524.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(19976..20764)
                     /gene="lpxA"
                     /locus_tag="ACHK07_000127"
     CDS             complement(19976..20764)
                     /gene="lpxA"
                     /locus_tag="ACHK07_000127"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO8100525.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20768..21223)
                     /gene="fabZ"
                     /locus_tag="ACHK07_000128"
     CDS             complement(20768..21223)
                     /gene="fabZ"
                     /locus_tag="ACHK07_000128"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO8100526.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21328..22353)
                     /gene="lpxD"
                     /locus_tag="ACHK07_000129"
     CDS             complement(21328..22353)
                     /gene="lpxD"
                     /locus_tag="ACHK07_000129"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO8100527.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22357..22842)
                     /gene="skp"
                     /locus_tag="ACHK07_000130"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22357..22842)
                     /gene="skp"
                     /locus_tag="ACHK07_000130"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO8100528.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(22964..25396)
                     /gene="bamA"
                     /locus_tag="ACHK07_000131"
     CDS             complement(22964..25396)
                     /gene="bamA"
                     /locus_tag="ACHK07_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO8100529.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVQVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25426..26778)
                     /gene="rseP"
                     /locus_tag="ACHK07_000132"
     CDS             complement(25426..26778)
                     /gene="rseP"
                     /locus_tag="ACHK07_000132"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO8100530.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26790..27647)
                     /gene="cdsA"
                     /locus_tag="ACHK07_000133"
     CDS             complement(26790..27647)
                     /gene="cdsA"
                     /locus_tag="ACHK07_000133"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8100531.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27660..28418)
                     /gene="ispU"
                     /locus_tag="ACHK07_000134"
                     /gene_synonym="uppS"
     CDS             complement(27660..28418)
                     /gene="ispU"
                     /locus_tag="ACHK07_000134"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO8100532.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28607..29803)
                     /gene="ispC"
                     /locus_tag="ACHK07_000135"
     CDS             complement(28607..29803)
                     /gene="ispC"
                     /locus_tag="ACHK07_000135"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO8100533.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(29895..30452)
                     /gene="frr"
                     /locus_tag="ACHK07_000136"
     CDS             complement(29895..30452)
                     /gene="frr"
                     /locus_tag="ACHK07_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO8100534.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30602..31327)
                     /gene="pyrH"
                     /locus_tag="ACHK07_000137"
     CDS             complement(30602..31327)
                     /gene="pyrH"
                     /locus_tag="ACHK07_000137"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO8100535.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31474..32325)
                     /gene="tsf"
                     /locus_tag="ACHK07_000138"
     CDS             complement(31474..32325)
                     /gene="tsf"
                     /locus_tag="ACHK07_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO8100536.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDSGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32460..33185)
                     /gene="rpsB"
                     /locus_tag="ACHK07_000139"
     CDS             complement(32460..33185)
                     /gene="rpsB"
                     /locus_tag="ACHK07_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO8100537.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33553..34347
                     /gene="map"
                     /locus_tag="ACHK07_000140"
     CDS             33553..34347
                     /gene="map"
                     /locus_tag="ACHK07_000140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO8100538.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34409..37081
                     /gene="glnD"
                     /locus_tag="ACHK07_000141"
     CDS             34409..37081
                     /gene="glnD"
                     /locus_tag="ACHK07_000141"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO8100539.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37112..37936
                     /gene="dapD"
                     /locus_tag="ACHK07_000142"
     CDS             37112..37936
                     /gene="dapD"
                     /locus_tag="ACHK07_000142"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO8100540.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTKIYDRE
                     TGEVHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38250..38636
                     /gene="yaeH"
                     /locus_tag="ACHK07_000143"
     CDS             38250..38636
                     /gene="yaeH"
                     /locus_tag="ACHK07_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO8100541.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(38725..39882)
                     /gene="cdaR"
                     /locus_tag="ACHK07_000144"
     CDS             complement(38725..39882)
                     /gene="cdaR"
                     /locus_tag="ACHK07_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO8100542.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40037..41461)
                     /gene="degP"
                     /locus_tag="ACHK07_000145"
     CDS             complement(40037..41461)
                     /gene="degP"
                     /locus_tag="ACHK07_000145"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO8100543.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41591..43108)
                     /gene="dgt"
                     /locus_tag="ACHK07_000146"
     CDS             complement(41591..43108)
                     /gene="dgt"
                     /locus_tag="ACHK07_000146"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO8100544.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43192..43890
                     /gene="mtnN"
                     /locus_tag="ACHK07_000147"
     CDS             43192..43890
                     /gene="mtnN"
                     /locus_tag="ACHK07_000147"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO8100545.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            43883..44683
                     /gene="btuF"
                     /locus_tag="ACHK07_000148"
     CDS             43883..44683
                     /gene="btuF"
                     /locus_tag="ACHK07_000148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO8100546.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIEQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            44721..45344
                     /gene="yadS"
                     /locus_tag="ACHK07_000149"
     CDS             44721..45344
                     /gene="yadS"
                     /locus_tag="ACHK07_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO8100547.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45391..45735)
                     /gene="erpA"
                     /locus_tag="ACHK07_000150"
     CDS             complement(45391..45735)
                     /gene="erpA"
                     /locus_tag="ACHK07_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO8100548.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(45728..45793)
                     /gene="yadW"
                     /locus_tag="ACHK07_000151"
     CDS             complement(45728..45793)
                     /gene="yadW"
                     /locus_tag="ACHK07_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO8100549.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(45817..47238)
                     /gene="clcA"
                     /locus_tag="ACHK07_000152"
     CDS             complement(45817..47238)
                     /gene="clcA"
                     /locus_tag="ACHK07_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO8100550.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47250..47297)
                     /gene="yadX"
                     /locus_tag="ACHK07_000153"
     CDS             complement(47250..47297)
                     /gene="yadX"
                     /locus_tag="ACHK07_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO8100551.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47463..48743
                     /gene="hemL"
                     /locus_tag="ACHK07_000154"
     CDS             47463..48743
                     /gene="hemL"
                     /locus_tag="ACHK07_000154"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO8100552.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(48778..50760)
                     /gene="fhuB"
                     /locus_tag="ACHK07_000155"
     CDS             complement(48778..50760)
                     /gene="fhuB"
                     /locus_tag="ACHK07_000155"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO8100553.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFLQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(50757..51647)
                     /gene="fhuD"
                     /locus_tag="ACHK07_000156"
     CDS             complement(50757..51647)
                     /gene="fhuD"
                     /locus_tag="ACHK07_000156"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO8100554.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51647..52444)
                     /gene="fhuC"
                     /locus_tag="ACHK07_000157"
     CDS             complement(51647..52444)
                     /gene="fhuC"
                     /locus_tag="ACHK07_000157"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO8100555.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLEAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVESLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52495..54684)
                     /gene="fhuA"
                     /locus_tag="ACHK07_000158"
     CDS             complement(52495..54684)
                     /gene="fhuA"
                     /locus_tag="ACHK07_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO8100556.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTIEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(54904..57438)
                     /gene="mrcB"
                     /locus_tag="ACHK07_000159"
     CDS             complement(54904..57438)
                     /gene="mrcB"
                     /locus_tag="ACHK07_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO8100557.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(57634..60063)
                     /gene="hrpB"
                     /locus_tag="ACHK07_000160"
     CDS             complement(57634..60063)
                     /gene="hrpB"
                     /locus_tag="ACHK07_000160"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO8100558.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRMEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGISLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60137..60667
                     /gene="thpR"
                     /locus_tag="ACHK07_000161"
     CDS             60137..60667
                     /gene="thpR"
                     /locus_tag="ACHK07_000161"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO8100559.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCIQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            60682..61386
                     /gene="sfsA"
                     /locus_tag="ACHK07_000162"
     CDS             60682..61386
                     /gene="sfsA"
                     /locus_tag="ACHK07_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO8100560.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61564..62019
                     /gene="dksA"
                     /locus_tag="ACHK07_000163"
     CDS             61564..62019
                     /gene="dksA"
                     /locus_tag="ACHK07_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO8100561.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62086..62982
                     /gene="gluQRS"
                     /locus_tag="ACHK07_000164"
     CDS             62086..62982
                     /gene="gluQRS"
                     /locus_tag="ACHK07_000164"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO8100562.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPGNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63042..64439
                     /gene="pcnB"
                     /locus_tag="ACHK07_000165"
     CDS             63042..64439
                     /gene="pcnB"
                     /locus_tag="ACHK07_000165"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO8100563.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64436..64915
                     /gene="folK"
                     /locus_tag="ACHK07_000166"
     CDS             64436..64915
                     /gene="folK"
                     /locus_tag="ACHK07_000166"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO8100564.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIESQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            65257..65853
                     /locus_tag="ACHK07_000167"
     CDS             65257..65853
                     /locus_tag="ACHK07_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100565.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIEPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            65940..66665
                     /locus_tag="ACHK07_000168"
     CDS             65940..66665
                     /locus_tag="ACHK07_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8100566.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            66707..69307
                     /locus_tag="ACHK07_000169"
                     /pseudo
     CDS             66707..69307
                     /locus_tag="ACHK07_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            69321..69869
                     /locus_tag="ACHK07_000170"
     CDS             69321..69869
                     /locus_tag="ACHK07_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100567.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLVASDCSNGYSKIFTTLTGTTT
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWTADEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            69884..70498
                     /locus_tag="ACHK07_000171"
     CDS             69884..70498
                     /locus_tag="ACHK07_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100568.1"
                     /translation="MKLKVIATLIATVAVGVSVSNNLTYASTTSASLTVNSNLTMGTC
                     SAKVVDTSGAVIDTVVLGNVYISELVAKSKVQPFKIRFSDCAGLPGKSAQLVLAPRAN
                     VGCAGGMSNTAAFSNQLNSTTDGGSSRAAVEVWTTTSPEGNGSEQFNCYQRNVINVDL
                     SNASTTQPYDFDLSARMIIAEGWSVNDVLPGRFLSPTTFTITYQ"
     gene            70519..71109
                     /locus_tag="ACHK07_000172"
     CDS             70519..71109
                     /locus_tag="ACHK07_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8100569.1"
                     /translation="MLCRHHKFVRFLGLATALITPFAYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71121..72233
                     /locus_tag="ACHK07_000173"
     CDS             71121..72233
                     /locus_tag="ACHK07_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8100570.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIERNAEGPYDMV
                     INPKPFSIQSAMVTVPEEIGFATWDSYIDLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSHGDALLKTTVPGIVYSVELVCTSCGAAEELDLYLPAQSGADNHTDNASSKWA
                     YEYSDESWYLRFRFFWTPEFKPQNGVSEGTAIPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTNGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAVWSN
                     NLLLLPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            72369..73163
                     /gene="panB"
                     /locus_tag="ACHK07_000174"
     CDS             72369..73163
                     /gene="panB"
                     /locus_tag="ACHK07_000174"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO8100571.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73175..74026
                     /gene="panC"
                     /locus_tag="ACHK07_000175"
     CDS             73175..74026
                     /gene="panC"
                     /locus_tag="ACHK07_000175"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO8100572.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74122..74331)
                     /locus_tag="ACHK07_000176"
     CDS             complement(74122..74331)
                     /locus_tag="ACHK07_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100573.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(74400..75272)
                     /gene="rpnC"
                     /locus_tag="ACHK07_000177"
     CDS             complement(74400..75272)
                     /gene="rpnC"
                     /locus_tag="ACHK07_000177"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO8100574.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            75546..75926
                     /gene="panD"
                     /locus_tag="ACHK07_000178"
     CDS             75546..75926
                     /gene="panD"
                     /locus_tag="ACHK07_000178"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO8100575.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(75930..77159)
                     /gene="yadE"
                     /locus_tag="ACHK07_000179"
     CDS             complement(75930..77159)
                     /gene="yadE"
                     /locus_tag="ACHK07_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO8100576.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77223..77663)
                     /gene="yadI"
                     /locus_tag="ACHK07_000180"
     CDS             complement(77223..77663)
                     /gene="yadI"
                     /locus_tag="ACHK07_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8100577.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(77768..78538)
                     /gene="yadH"
                     /locus_tag="ACHK07_000181"
     CDS             complement(77768..78538)
                     /gene="yadH"
                     /locus_tag="ACHK07_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8100578.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78535..79461)
                     /gene="yadG"
                     /locus_tag="ACHK07_000182"
     CDS             complement(78535..79461)
                     /gene="yadG"
                     /locus_tag="ACHK07_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8100579.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79570..80232
                     /gene="can"
                     /locus_tag="ACHK07_000183"
     CDS             79570..80232
                     /gene="can"
                     /locus_tag="ACHK07_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO8100580.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80273..80809)
                     /gene="hpt"
                     /locus_tag="ACHK07_000184"
     CDS             complement(80273..80809)
                     /gene="hpt"
                     /locus_tag="ACHK07_000184"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO8100581.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81015..83405
                     /gene="gcd"
                     /locus_tag="ACHK07_000185"
     CDS             81015..83405
                     /gene="gcd"
                     /locus_tag="ACHK07_000185"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO8100582.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRYSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            83444..83542
                     /locus_tag="ACHK07_000186"
                     /pseudo
     CDS             83444..83542
                     /locus_tag="ACHK07_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(83607..85157)
                     /gene="cueO"
                     /locus_tag="ACHK07_000187"
     CDS             complement(83607..85157)
                     /gene="cueO"
                     /locus_tag="ACHK07_000187"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO8100583.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85323..85670
                     /gene="yacC"
                     /locus_tag="ACHK07_000188"
     CDS             85323..85670
                     /gene="yacC"
                     /locus_tag="ACHK07_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO8100584.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            85776..86642
                     /gene="speE"
                     /locus_tag="ACHK07_000189"
     CDS             85776..86642
                     /gene="speE"
                     /locus_tag="ACHK07_000189"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO8100585.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            86658..87452
                     /gene="speD"
                     /locus_tag="ACHK07_000190"
     CDS             86658..87452
                     /gene="speD"
                     /locus_tag="ACHK07_000190"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO8100586.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87490..87852)
                     /gene="yacL"
                     /locus_tag="ACHK07_000191"
     CDS             complement(87490..87852)
                     /gene="yacL"
                     /locus_tag="ACHK07_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO8100587.1"
                     /translation="MDYEFLRDTTGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88027..90624)
                     /gene="acnB"
                     /locus_tag="ACHK07_000192"
     CDS             complement(88027..90624)
                     /gene="acnB"
                     /locus_tag="ACHK07_000192"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO8100588.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEKEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            90979..92826
                     /gene="yacH"
                     /locus_tag="ACHK07_000193"
     CDS             90979..92826
                     /gene="yacH"
                     /locus_tag="ACHK07_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO8100589.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAILAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHDDDYHHHDGGHRDGNGWQHNGD
                     NINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQ
                     LPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRR
                     QPLTQQQKDAARQRYQSASPEPRQAVRERMQTNPQNQQRREAARERIQPASPEQRQAV
                     REKMQTNPQIQQRREAARERIQSASPEQRQVFKEKVQQSPLNQQQRDNARQRVQSASP
                     EQRQVFREKVKESHPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(92897..94321)
                     /gene="lpdA"
                     /locus_tag="ACHK07_000194"
     CDS             complement(92897..94321)
                     /gene="lpdA"
                     /locus_tag="ACHK07_000194"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO8100590.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94529..96421)
                     /gene="aceF"
                     /locus_tag="ACHK07_000195"
     CDS             complement(94529..96421)
                     /gene="aceF"
                     /locus_tag="ACHK07_000195"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO8100591.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96436..99099)
                     /gene="aceE"
                     /locus_tag="ACHK07_000196"
     CDS             complement(96436..99099)
                     /gene="aceE"
                     /locus_tag="ACHK07_000196"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO8100592.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99260..100024)
                     /gene="pdhR"
                     /locus_tag="ACHK07_000197"
     CDS             complement(99260..100024)
                     /gene="pdhR"
                     /locus_tag="ACHK07_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO8100593.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYARREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24307 a        27180 c        24730 g        24047 t
ORIGIN      
        1 tcttcaaccg aaccggctgt ttgtgtgaag tgattcacat ccgccgtgtc gatggaggcg
       61 cattataggg agtcggctca ggaagacaag cggaaaaatg catttttatt tcaaccgctc
      121 atcttttaat cattgcgccg gtttttgctg cttttttatc gcttgcggca ggtctgccag
      181 gctatttaac acccaatctg ccgcgttttc tgcttcaggt gtaataggtt tacccgtacg
      241 caccagcact tttgttccca cgttcgccgc aaccgctgcc tgcatatctt ctaatttatc
      301 gcccaccata taagaagcgg ccatatcaat atgcaaataa tcgcgtgctg acaaaagcat
      361 ccccggatgt ggtttgcggc aatcgcagac ctggcgaaac tcttcaacac taccctgcgg
      421 atgatgcggg caataataga taccatccag atcgacatct cggtccgcca gcgaccagtc
      481 catccactcg gtcagcgttt caaactgtgc ttcggtaaat ttaccgcgag caatgccaga
      541 ctggttggtt actaccacca gcgcaaagcc catttttttt agctcgcgca tggcgtcaat
      601 aacaccgttg ataaattcaa agttgtcgat ctcatggaca tagccgtgat cgacattaat
      661 ggtgccatca cggtcaagaa aaattgcggg tacgctcttc gccacctttt atagctcctt
      721 aataaggcat gtgacgctag tatcgcatgt ttcgacctgc aagaaagtgc tcttcgcata
      781 aacctgattg atttagacgt ctggatgcct taacatccat ttcattgacg gcgttgcccg
      841 tttcaggcat tcgagatgcc acgactaact taatgacgat aataaataat caatgataaa
      901 actttcgaat atcaccaaag tgttccacca gggcacccgc atcatccagg cgttgaacaa
      961 cgtcagcctg catgtgccag ctggacaaat ttatggcgtt atcggtgcct caggcgcggg
     1021 taagagtacg cttatacgtt gtgtaaacct gctggagcgc ccaaccgagg gtagcgtgct
     1081 ggtcgatggc caggaactga ccacgctgtc agaatccgag ttgaccaaag ctcgtcgcca
     1141 gattggtatg attttccagc attttaacct gctctcttcg cgtactgttt ttggcaacgt
     1201 ggctctgccg ctggagctgg acaatacacc gaaagacgag atcaaacgtc gcgtgacgga
     1261 attgctgtca ttggttggtc ttggcgataa gcatgatagc tacccgtcga atctttccgg
     1321 tgggcagaaa caacgtgtgg cgattgcccg tgcattagcc agcaatccca aagtattgct
     1381 gtgtgatgaa gccaccagcg cgctggaccc ggcaacgaca cgttctattc tcgaactgct
     1441 gaaagacatc aaccgccgtc tgggtttgac gattctgttg atcacccacg aaatggacgt
     1501 tgtgaagcgt atttgtgatt gcgttgcggt catcagcaat ggcgaactaa tcgagcagga
     1561 cacggtaagt gaagtgttct cacatccgaa aacgccgctg gcgcagaagt ttattcagtc
     1621 aaccctgcat ctggatatcc cggaagatta ccaggaacgt ctgcaagcgg agccattcac
     1681 tgactgcgtg ccgatgctgc gtctggagtt taccggtcaa tcggtcgatg ccccactgct
     1741 ttctgaaacc gcgcgtcgtt tcaacgtcaa caacaacatt attagcgcgc agatggacta
     1801 cgctggtggc gttaaattcg gcatcatgct gactgaaatg cacggcacac aacaagatac
     1861 gcaagccgcc attgcctggc tgcaggaaca ccatgtaaaa gtagaggtac tgggttatgt
     1921 ctgagccgat gatgtggctg ctggttcgtg gcgtatggga aacgctggca atgaccttcg
     1981 tatccggatt ttttgggttt gtgattggtc tgccggttgg cgtcctgctt tatgtcacgc
     2041 gtccggggca aattattgct aacgcgaagc tgtatcgtac cgtttctgcg attgtgaaca
     2101 ttttccgttc catcccgttc attatcttgc tggtatggat gattccgttt acccgcgtta
     2161 ttgtcggtac atcgattggt ttgcaggcag caattgttcc gttaaccgtt ggtgcagcac
     2221 cgtttattgc ccgtatggtc gagaacgctc tgctggagat cccaaccggg ttaattgaag
     2281 cttcccgcgc aatgggtgcc acgccaatgc agatcgtccg taaagtgctg ttaccggaag
     2341 cgttgccggg tctggtgaat gcggcaacta tcaccctgat taccctggtc ggttattccg
     2401 cgatgggtgg tgcagtcggt gccggtggtt taggtcagat tggctatcag tatggctaca
     2461 tcggctacaa cgcgacggtg atgaatacgg tactggtatt gctggtcatt ctggtttatt
     2521 taattcagtt cgcaggcgac cgcatcgtcc gggctgtcac tcgcaagtaa cgttcaacac
     2581 aacataaata attgaagaag gaataaggta tggcgttcaa attcaaaacc tttgcggcag
     2641 tgggagccct gattggatca ctggcactgg taggctgcgg tcaggatgaa aaagatccaa
     2701 accacattaa agtcggcgtg attgttggtg ccgaacagca ggttgcagaa gtcgcgcaga
     2761 aagttgcgaa agacaaatat ggcctggacg ttgagctggt aaccttcaac gactatgttc
     2821 tgccaaacga agcattgagc aaaggcgata tcgacgccaa cgccttccag cataaaccgt
     2881 accttgatca gcaactgaaa gatcgtggct acaaactggt cgcagtaggc aacacttttg
     2941 tttatccgat tgctggttac tccaagaaaa tcaaatcact ggatgaactg caggatggtt
     3001 cgcaggttgc cgtgccaaac gacccaacta accttggtcg ttcactgctg ctgctgcaaa
     3061 aagtgggctt gatcaaactg aaagatggcg ttggcctgct gccgaccgtt cttgatgttg
     3121 ttgagaaccc aaaaaatctg aaaattgttg aactggaagc accgcaactg ccgcgttctc
     3181 tggacgatgc gcaaatcgct ctggcagtta tcaataccac ctatgccagc cagattggcc
     3241 tgactccagc gaaagacggt atctttgtcg aagataaaga ctccccgtac gtaaacctga
     3301 tcgtaacgcg tgaagataac aaagacgccg agaacgtgaa gaaattcgtc caggcttatc
     3361 agtctgacga agtttacgaa gcagcaaaca aagtgtttaa cggcggcgct gttaaaggct
     3421 ggtaattttt aggctgtttc cacaatttgt aatatcattc aggacgggcg cttgcccgtc
     3481 ttgtcatttt tacaagctcc tgattcaata ttgacgtttt gatcatacat tgaggaaata
     3541 ctatgcgtgc tttaccgatc tgtttagtag cactcatgct aagcggctgt tccatgttaa
     3601 gcagatcccc tgtcgaaccc gttcaaagca ctgcacccca gccgaaagcg gagcctgcaa
     3661 aaccgaaagc gccgcgcgcc acgccggtcc gaatttatac caatgcagaa gaattagtcg
     3721 gcaaaccgtt ccgcgatctc ggtgaagtca gtggcgactc ttgccaggcc tctaatcagg
     3781 actctccgcc gagcattcca accgcgcgta agcggatgca aatcaacgcc tcgaaaatga
     3841 aagccaatgc ggtattactg catagctgcg aagtcaccag cggtacgcca ggctgctatc
     3901 gtcaggctgt atgtatcggt tctgcgctta acattacggc gaaatgagca gtttccagtt
     3961 cgagcaaata ggcgttattc gctcgcccta taaagaaaaa ttcgccgttc cgcgccagcc
     4021 aggtctggta aaaagcgcca acggcgaact gcatctcatt gctccctaca accaggccga
     4081 cgccgttcgc ggcctggaag cattcagcca tttatggata cttttcgtct ttcatcaaac
     4141 gatggaaggc ggctggcgtc cgactgtgcg tccgcctcgt cttggcggta atgccagaat
     4201 gggtgttttc gctacgcgtt ccacttttcg ccctaaccca attggtatgt cgctggtaga
     4261 gctgaaagag gttgtttgcc ataaagacag cgtgattctg aagctcggca gtctggatct
     4321 ggtcgatggt acgccggtgg tggatatcaa accgtatctc ccctttgccg aatcgcttcc
     4381 cgatgccagt gccagctatg cgcaaagtgc gccagcggca gagatgacgg tgagttttac
     4441 cgcagaggtc gaaaagcagc ttttgacgct ggagaagcgt tatccgcagt taacgctgtt
     4501 tatccgcgaa gtactggcgc aggacccgcg cccggcctat cgtaaaggtg aggaaacggg
     4561 caaaacctat gccgtctggc tacatgattt taacgttcgc tggcgcgtca ccgacgcagg
     4621 ttttgaagtc tttgcgctgg aaccgcgtta aattcacgcc cttctctttt gacatttctt
     4681 ttgcactggt aaactaaatc actttttttg tcccaggctc gccttgagcc tgttctacct
     4741 tccaactgga accgtaacaa catgcgtact agccaatacc tgctctccac tctcaaggag
     4801 acacctgccg acgccgaggt gatcagccat cagctgatgc tgcgcgccgg gatgatccgc
     4861 aagctggcct ccgggttata tacctggctg ccgaccggcg tgcgcgttct gaaaaaagtc
     4921 gaaaacatcg tgcgtgaaga gatgaacaac gccggtgcga tcgaggtgtc gatgccggtg
     4981 gttcagccag ccgatttgtg gcaagagagt ggtcgttggg aacagtacgg tccggaactg
     5041 ctgcgttttg ttgaccgtgg cgagcgtccg ttcgtactcg gcccaactca tgaagaagtt
     5101 atcactgacc tgattcgtaa cgagcttagc tcttacaaac agctgccgct gaacttctat
     5161 cagatccaga ccaagttccg cgatgaagtg cgtccgcgtt tcggcgtcat gcgttcccgc
     5221 gaattcctga tgaaagatgc ttactctttc catacttctc aggaatccct gcaggaaacc
     5281 tacgatgcaa tgtatgcggc ctacagcaaa atcttcagcc gcatggggct ggatttccgc
     5341 gccgtacagg ccgacaccgg ttctatcggc ggcagcgcct ctcacgaatt ccaggtgctg
     5401 gcgcagagcg gtgaagacga tgtggtcttc tccgacacct ctgactatgc agcgaacatt
     5461 gaactggcag aagctatcgc gccgaaagaa ccgcgcgctg ctgctaccca ggaaatgacg
     5521 ctggttgata cgccgaacgc gaaaaccatc gcggaactgg ttgaacagtt caatctgccg
     5581 attgagaaaa cggttaagac tctgctggtt aaagcggttg aaggcagtag cttcccgctg
     5641 gttgcactgc tggtgcgcgg tgaccacgaa ctgaacgaag ttaaagcaga aaaactgccg
     5701 caggttgcaa gcccgctgac tttcgcgacc gaagaagaaa ttcgtgccgt ggttaacgcc
     5761 ggtccgggtt cactgggtcc ggtaaacatg ccgattccgg tggtgattga ccgtaccgtt
     5821 gcggcgatga gtgatttcgc tgctggtgct aacatcgatg gtaaacacta cttcggcatc
     5881 aactgggatc gcgatgtcgc taccccggaa gttgcggata tccgtaacgt ggtggctggc
     5941 gatccaagcc cggatggcca gggtacgctg ctgatcaaac gtggtatcga agttggtcac
     6001 atcttccagc tgggtaccaa gtactccgaa gcactgaaag cctccgtaca gggtgaagat
     6061 ggccgtaacc aaatcctgac gatgggttgc tacggtatcg gggtaacgcg tgtggtagct
     6121 gcggcgattg agcagaacta cgacgaacgc ggcatcgtat ggcctgacgc tatcgcgccg
     6181 ttccaggtgg cgattctgcc gatgaacatg cacaaatcct tccgcgtaca agagcttgct
     6241 gagaaactgt acagcgaact gcgcgcgcaa ggtatcgaag tgctgctgga tgaccgcaaa
     6301 gagcgtccgg gcgtgatgtt tgctgatatg gaactgatcg gtattccgca cactattgtg
     6361 ctgggcgacc gtaacctcga caacgacgat atcgaatata aatatcgtcg caacggcgag
     6421 aaacagttaa ttaagactgg tgacatcgtc gaatatctgg tgaaacagat taaaggctga
     6481 tgccagaaag ggtcctgaat ttcagggccc ttttttacat ggattgtata aaatggataa
     6541 accaaaggcg tactgccgcc tgcttctccc ctgctttctt ttactttcgg cctgtaccgt
     6601 tgatatcagc cagccagact cctctgccac agcggtagat gtcgaggcaa aaacatgggc
     6661 tgtcaaattc cagcatcaaa gctctttcac cgaacaagca ataaaagaaa taacggtacc
     6721 tgatctcaaa cccggcgatt tgctgttctc ctcaagcctt ggggtaacct catttggaat
     6781 ccgcgtcttc agcacttcct ctgtgagtca cgttgcaatc tatctgggtg aaaataacgt
     6841 tgcagaagcg acaggcgctg gcgtccagat tgtttccctt aaaaaggcga tgaagcatag
     6901 cgataagctt ttcgtcttac gagtcccgga tcttaccccg caacaagcca cagaaatcac
     6961 cgcgttcgcc aacaaaatta aagatagcgg gtataactat cgcggcattg tcgagttcat
     7021 tccatttatg gtgacccgcc agatatgctc actgaatcct ttttccgcag attttcgcca
     7081 acagtgcgtc agcggcctgg cgaaagcgca gttaagcagt gtgggcgaag gagacaaaaa
     7141 gtcgtggttt tgttcggaat ttgtcactga tgcatttgcc aaagccgggc atccgcttac
     7201 gctggcgcaa tcgggctgga ttagccctgc cgatctgatg catatgcgta ttggtgatgt
     7261 ctctgcgttt aagccagaaa cgcagttaca gtatgttggg cacctgaagc cagggattta
     7321 tatcaaggcg ggtcgctttg ttggtttgac ccggtgataa agtaaatgcc ttatccgcct
     7381 taaggtatga ggggggttgt aggcatgata agacgcgcca gcgtcgcatc aggcgttgag
     7441 caccattgcc ggatgcggcg taaacgcctt atccggccta caaaaaacgt gcaaattcaa
     7501 aatgttgaaa tatatccttc tggcctgttt tgcgtttgtt tctgtctcaa gacgggttac
     7561 tgccccaaac tactgcaatc ctggtttggg taaaatttcc ctgccgtatc gggtgctaat
     7621 actttggttg gcgcaccggt atccggatta gcctcaagcg taaagtgacc ttctactgac
     7681 agtaacaccg gtttttcact gtggccgcgc gcagccaggt agccacgctc cagctctgcg
     7741 ttattcgcta ccatgaaacg ttttccggtc gcgcaatcag tgaaggtcgc cgcatcagcc
     7801 atataaaaat acatgccccg cagggtcatc ggcgtcatgg gtaaactgga ttgtgccggt
     7861 tccagcgtat agttgaactg cgattcaatc ggattgcctt cacgatcgag catctccagc
     7921 gcatcgcctt tcgcccgata atatgacttt tcacctttgc tgtcggttaa taccagcttg
     7981 tcagcggttc gcgcccatgt accgtaggaa gcgaaggagg aaggttcttc acgagcaccg
     8041 agataacgct cattcatcac ccatgttccg tctttttcga ggaacagaga ggtttcgatt
     8101 ccttcgcaat cggcacacgg cagcacgccg cgccaacttt gcggcatcgg tttcagttcg
     8161 gcaacctgcg ccggagaaag cgtatcgact tcggcccgat tattacaacc catcagagta
     8221 aagaggctga ttacagccat cgctgtcact atcgcttttt tcaccatcca ttccttcttt
     8281 ttattcccga ccgctgcgca ctttgccgcg catcgccttc acgcttgatt tttgtgcttt
     8341 cgatgccagc ctgcgctctt tcgatgcacg ggtgggccgc gtgggtcgtc gggctttttg
     8401 ttctgttgtt aattctttaa tcacagccac cagccgggcc agagctgctt cgcggttcag
     8461 ttcctgactg cggtattcct gtgccttaat gacaatcacg ccatcactgc tgatcaaatg
     8521 atggctggcg gcgagcagac gctctttgta atactctggc aggctggacg cccgaatgtc
     8581 aaaacgcaga tgaatagccg ttgaggtctt attaacatgc tgcccgcccg cgccctgcgc
     8641 acgaatggcg gtgatctcaa gctcaccatc gggaatagca acatgtcggg aaatcacaat
     8701 catgaaggtt gttgccaggc ggttaagttc acttccagat tattcttatc atccgataac
     8761 cagatcacgc catcctgaat cgttgcctgc agcgtcatgg tacgatcggc aaaggcgctt
     8821 actttcgcca gttgttcatc gtccagatac cagacggaaa gattggcaaa ctgcacacat
     8881 ttgctctgat tttgctgcca ccagatttgc gccgcccgac tattataggt atgaagggtg
     8941 gcgcgccagc gtcagagagg catcgagaaa ctggttgcgg tcgagatcgg ccacattaac
     9001 cgtcgcttta taaattgtcg ctttaagcgc cataagaaaa ctcctttcga atcaggaggc
     9061 cattgtaacg gaatacgtgc agttgcggcg ctgatattgc agaaatgagt gtggtatagt
     9121 caccttgcga aacagagttt atcttcgtag gcttagactt gcatccacgg ttaagtcaga
     9181 gtgctgacag gagggcatgt ggaaaaatat tgtgagttaa tacgcaagcg gtacgcggaa
     9241 atcgccagcg gagacttagg atacgttccg gacgcgctgg gctgcgtttt gaaagtgctg
     9301 aatgaaatgg ctgccgacga cgccctttct gaggctgtca gggaaaaagc agcatacgct
     9361 gcggcaaact tactggtgag cgattatgtc aatgaatgat acgtatcaac caatcaattg
     9421 tgatgattac gataatcttg agctcgcctg ccagcatcat ttaatgctga cacttgagct
     9481 gaaagatggc gaaaaattgc aggcaaaagc cagcgattta gtctcccgca aaaatgtgga
     9541 gtatctggtc gtcgaggccg ctggcgaaac ccgtgagcta cgtctggata aaattaccag
     9601 ctttagccac ccggaaatcg gtacggtggt ggtaagcgaa tcctgattga cttttgccgg
     9661 atgcgacgtt tgatgcgtct tatccggctt tcacttaact aagcgttttc tgccagacaa
     9721 aacttacgcc cttctcacct tcagccacac cttcttgcgt cacaaatacc cctgccgccg
     9781 caatcagcgt ttcgccataa aacagcagtg gcgtggtgtc acgtagccac ggcggcacgc
     9841 ccagctcctg ccagattttc ttcagcttac gtccgccgtt gcgaccgaca atatgcagca
     9901 atcctggcgc tttgaaacgc acgctgaccg cttcgtctgc acgcggaggg cgaatatcgc
     9961 ctcccgcagt aagctgtaca cttcccagcc ccgccggtaa ttccagcggt tgtagccacg
    10021 tctgccacgg cacaatggtt tcgctttgcc cggcaacgga tttaatccac cacagttgcg
    10081 attgatagcg gcgtatttca aacgcgccaa agcgtaaaca gggggaggca tcttcccgcg
    10141 ccagcgccac ttcctgccag atccgcacca gcgcgtcgcg ggaaggcatc ggtgcattct
    10201 gccctgccag ccagcggcgg ataattgccg cgcgacgggc atcactcatc gccagcattg
    10261 gcgcaatctg cagcgtcccc tgcggcgttt ggcagtgcgc taaatcatct gccagcaatt
    10321 catccagcag gctctcttgt tcagcacaaa gtgcggcgct gcgagccgtt gcttcggcaa
    10381 aatgcggcca acgctgctgc aataacggca ctacacgcag gcgcagaaag ttacgatcgt
    10441 atgagtcgtc ctgattactt tcgtcttcaa tccagcgtaa accatgcgct aatgcccact
    10501 gcgccagttc cccccgcgtg cgggcgagca acgggcggat aagccgtgtt ccggcaaact
    10561 ccgaaacttc cgccatagcc gaaagcccgg cagggccgct accacgtttt agcgccagca
    10621 gaaaggtttc acattgatcg tcgagatgtt gcgcggtgac cagcacttca ccgggcaaca
    10681 aggtgcgggc aaatgcctga taacgcgcct gccgcgcctg ggcctcaatg cccagtcctt
    10741 cttgcgcaag ttgcacgcgc tcgaccacca gcggcacctg ccactgttgg cagatgttct
    10801 cgcaatgcct aacccaggca tcggcattgg cacttaaacc gtgatgcaca tggatagcgc
    10861 gtagagtgac gcccggattt tccgtccgcc actgcaccaa ctgatgcagc agaacggtgg
    10921 agtcaagccc gccgctaaag gccaccagaa tctggcgtga ggtgagaagt tgtctattga
    10981 gcgtgagtgt catgatggtg cgattttaca gtagcaatgc ccggcacgtt accgggcaaa
    11041 tgccgcgatg acaagcctta ctgctcatac agttccaacg gcagcccgtc cggatcgtta
    11101 aagaaggtga agcgtttttg cgtgtatgga tcgacacgga tggcttcaca cttcacgtta
    11161 tggctttcaa ggtgcgccac tgccgcatcg atgtcatcaa cgctaaaagc cagatgacgc
    11221 aggccgcaag cttccggtcg gctggggcgc tccggcggga acggaaatga gaaaagctca
    11281 atcacatatt gcccattaag cgccaaatcc cctttccacg agtcgcgcgc ttcgcgatag
    11341 acttcgcttt gcagcgtgaa accaagaata tcgcagtaga aagctttgct caccgcataa
    11401 tccgtcgcaa taatcgcaat atggtgaacc tgttttaaac ccagcataac gtctccttta
    11461 tttgttaaca gcacgttact cgcccggaag ccgctctggc aagttatccc gccattttta
    11521 ggactcgtac gcggtaaacg ccgtcttcgt cctgtttcgc accgtgaata tccgtttcaa
    11581 aaccggggta atgttgccct acggaacaaa gcatcagtag aaaatcgagt actgtgcggc
    11641 tctctttggt cagcatttct ccaggcatca acagcggtac gcccggtgga taaggcagga
    11701 tcatatttgc cgatactcta ccgaccagtt gttccagcgc aatggtttct acttcacctt
    11761 taatctgtct ctgccatgcc tgatgtggcg tcatgatcat ctccggcaaa gtatcgaatg
    11821 cccgtaacat caaaccggga agatcgtgtt tacgaatcag cttatggatc ccttgcgcca
    11881 gatcctgaat acgcatattg cggtagaaat cgggatcttc agcatagaga tcgggcagca
    11941 tatttttgat ccgcaggttg agatcgtaag agcgcttaaa ttccgtcaac ccacgcaata
    12001 atcccattgc tttggtttta tcgatgccaa tactaaagag aaacagcagg ttataagggc
    12061 cggttttctc tactacgatc ccacgttcgt cgaggaattt tgccaccagc gccgccggga
    12121 tcccctcctc actcatattg ccctgttcgt ccatccccgg tgtcaaaata gtgactttaa
    12181 ccggatcgag aaacatatga tcggcatccg catcgttaaa gccgtgccac tgttcgccag
    12241 gcgcaacggg ccagcattcg gcttcatcca cctgcggcgg ttgccagata tcgaaaaacc
    12301 aaccgtcaga ctcttcccgc aaccgctgga cctctttgcg aaaatgcagc gcccgctcca
    12361 ccgaacggtt aatcagccgt ttacccggat taccacgcag catcgctgct gccgtctcaa
    12421 ccgaagcaac aatgggataa ctgggcgagg tggtggtatg catcataaag gcttcgttaa
    12481 aggcctcttc gtcatactcg cctttaatgt ggatcagcga agcctgcgat aacgccgcca
    12541 gcattttgtg ggtcgattgc gtttcgaaga tcacttttcc cgcaacacgc tcgccgctca
    12601 taccactttt cccctggtag atcggatgaa aatgggtgta cggcacccag gcggaatcga
    12661 agtggatcga cgggacatcc agcgtctgtt tgatccagtc ggtgttgtag agcaagccat
    12721 cataggttga gttggtaatc accgcatgaa ccggccattg tgcctgcgtg gtggcagcga
    12781 ctttctcttc gatgctgtca cgagtaaatt cacggcgcgg gatcccaccc agaatcccca
    12841 acgcattacg cgtcggtttc agccagactg gcactacatc gttcatcatc aacagatgcg
    12901 ccagtgattt atggcagttg cggtcgatca acagcgtact gccggatggc gcggcgtaca
    12961 tacccacaat tttgttcgac gtcgatgttc cgttggtaac gatataactc tgttccgcgc
    13021 cgaaagtccg cgcgatgtac tcttccgctt ccaggtgtgg cccggtgtgg tcgagcaacg
    13081 aaccaagctc ggtgaccgaa atagatacat cagccttaag agtattcccg ccgaaaaagt
    13141 cataaaacag acagccaacc gggctttttt gatatgcggt gccgcccata tgacccggcg
    13201 tacaaaaggt gtacttccgc tctttgacgt aggtaaacaa ggctttcgtg aacggtgggg
    13261 taatgttatc aagatattcg tcggtgtact gacgcatacg aatggcgata tcttccgcct
    13321 gccccagcgc atattcaaaa aaccagagcg ccatccgcat atcctgcacg ctgacatcca
    13381 tcgtcgagtg ggtgttgatg aaggcataaa gcgggagata ttcattaagc tgattgatat
    13441 cgctacataa atcgagactg tactcatccc agtcaaaaat cacgccgcaa attcgagggt
    13501 tatgttcgat aaatttcagc aaatcaacgc tgttttgtgg ccagataatc tgaaagcctt
    13561 gcgccaccag cgccgactcc agttctttaa tgggctcatc tttataaaag acgccatgcg
    13621 gtcccataat ggcaatgatg ttcatgtgtt cctcctggaa aatccttcct taatcatagc
    13681 ctgctcaaac cgtggcgata aaaaagggcc accgaagtga ccctttttca gaacttttgc
    13741 gaattacgcg taaccgtagc tcatcaggcg ctgataacga cgatttttta aatcttcagt
    13801 gcttaacacg tcgagatcgg ccagatccgc cagcagttgc gctttcaacg atgccgccat
    13861 cgcttccggg ttacggtgag caccacccag cggttccggg atgatggagt cgatcagttt
    13921 tagttctttc agacgcggag cgatgatacc catcgcttca gctgccagcg gtgctttgtc
    13981 ggcgctcttc cacagaatgg acgcacaacc ttccggcgag ataacggaat aggtgctgta
    14041 ttgcagcata ttcactttat cgcccacgcc aatcgccagc gcgccgccag aaccaccttc
    14101 accgataacc gtacaaacta ccggtacacc gaggcgagac atttcacgca ggttgcgtgc
    14161 aatagcttca gactgaccgc gttcttctgc gcccacgccc ggataagccc ccggggtgtc
    14221 gataaaggtg atgattggca tcttaaagcg ttcagccatt tgcatcaggc gcagagcttt
    14281 gcggtaacct tctggtgctg gcataccaaa gttacggcga attttttctt tggtttcacg
    14341 acctttttga tgaccaatga tcatcaccgg acgaccatcg agacgggcga taccaccgac
    14401 gatagcttta tcgtctgcat acgcgcggtc gccagccagt tcgtcaaatt catcaaatgc
    14461 caggcgaacg taatccaggg tataaggacg ctgtggatgg cgtgccagtt gcgcaatctg
    14521 ccatgcaccg agatcggcga agattttgcg cgtcagttct acactttttt cacgcagacg
    14581 atgcacttct tcatcgatgt taatatccag tttctcatcc tgacggctaa ccgcagtcag
    14641 agaatcgatt ttcgcttcca gctctgcaat cggctgttca aaatcaagga aattcagact
    14701 catagtattc ctgtattagt caaactccag ttccacctgc tccgaaccaa tgaggccacg
    14761 gagatcgttt aataaacgat cgctcggaga gacacgccac gtcgcgccaa aacgcaaccg
    14821 cgcgcgtgca tccgccctct gatagtagag atgtactgga attgtcccag agcggtgggg
    14881 ttccagagac tgacggagtc ggtttaaaag ctggtcatca atttgcctgt ccgtcagcga
    14941 gatagcaagc ccgcgagcat atttttcccg ggcttcgtca atatccatca cttcgcgagc
    15001 ggtcatttta agcccaccgc tgaagtcatc aaagctgacc tgtccgctga cgataagtat
    15061 gcggtctttt tccagcaatt gctggtattt atccagggcg tcagtaaaca acatcacttc
    15121 cagccgcccg gaacggtcat ccagcgtgca gataccgata cgattgccgc gcttggtgac
    15181 cataacccgc gcggcaacaa cgagccccgc agccgtgatg actttaccac gttctgtcgg
    15241 gtgcatgtct ttcagcctta cgcctccgac ataacgctca atctctttca aatactggtt
    15301 gatagggtgt cccgtcaggt acagacctaa cgtttcacgt tccccatcta ataccacctg
    15361 ctccggccac ggttggcagc tggcgtagga ttgttcaatt tgttccggct cttcggccag
    15421 cacgccgaac atatcggcct gaccgatggc ttccgctttc gcgtgttgat ctgccgcttt
    15481 taacgcatcg cccagcgagt tcatcagcgc tgcgcggtgt ggcccaagac ggtcaaacgc
    15541 cccggacata atcagttttt ccaacacccg ccggtttaac tttttggtgt cggttcgggc
    15601 gcagagatca aacagttcgc ggaagtagcc gcctttatta cgggcttcga tgatcgcctc
    15661 aatcggacct tcacctaccc ctttgatcgc gccaatacca tacacgattt cgccgtcgtc
    15721 gttgacgtgg aaatggtaaa gaccggagtt tatatctggt ggcaggattt tcagccccat
    15781 ccgccagcac tcatccacca ggcccaccac cttctcggtg ttgtccatat cggcggtcat
    15841 taccgccgcc ataaactctg ccggatagtg cgctttcagc cataatgttt gatatgacac
    15901 caaagcatag gccgcagagt gcgatttgtt aaatccgtaa cccgcgaatt tctccaccag
    15961 gtcgaagatt ttcatcgcca gttcggcgtt gattccgttc ttttctgcac cttcagcaaa
    16021 tacagaacgc tgcttcgcca tctcttccgg ctttttctta cccatcgcac gacgcagcat
    16081 atccgcgcca ccgagggtat aaccagaaag cacctgcgca atctgcatga cctgttcctg
    16141 atacaggata atgccgtagg ttggctccag taccggtttc aggctttcat gctgccactg
    16201 tacgtctgga taggagattt cttcgcgacc atgtttacgg tcaataaagt tatccaccat
    16261 ccctgactgc aacggaccag ggcggaacag tgccaccagt gcgatcatat cttcgaagca
    16321 gtcaggttgc agacgcttga tcaggtcctt catgccgcgc gattcaagct ggaataccgc
    16381 cgtggtttcc gagcgttgca gcatgtcgaa gcttttcttg tcatccagcg ggatcgcggc
    16441 gatatccagc ggcggctcgc cattcttcgc ccgccgcttg ttgatcatct ccagcgccca
    16501 gttgatgatg gtgagcgtac gcaaaccaag gaagtcgaac ttcaccagtc cggcgtattc
    16561 aacgtcgctt ttatcaaact gggtgaccgg atgtttgccc tcttcatcgc aataaagcgg
    16621 cgcaaaatcg gtaattttgg tcggcgcgat aaccacccca ccggcgtgct taccggcgtt
    16681 acgggtgacc ccttccagtt tgcgcgccat gtcgatcagc gccttaactt cttcatccgc
    16741 ttcgtagatt tccggcagct gcggctcggc ttcaaacgct ttcgccagcg tcatccccgg
    16801 atcgggcggg atcagtttcg agatacgatc gacaaagccg tacggatgcc ccagcacgcg
    16861 gcctacgtcg cggatcaccg ctttcgccgc catcgtaccg aaggtgataa tctgcgatac
    16921 cgcatcacga ccgtacatgt ccgctacgtg ttcgataacc tgatcgcgtt tctccataca
    16981 gaagtcaacg tcgaagtcag gcatggagac acgttccggg ttaaggaaac gttcgaacag
    17041 caggtcaaat tccagcggat cgaggtcggt gattttcagc gcgtaggcca ccagtgagcc
    17101 tgcgccggag ccacggcctg gccctaccgg tacaccgtta tctttcgacc actggataaa
    17161 ttccataacg ataaggaagt agcccgggaa gcccatctgg ttgataacct gaagttcagt
    17221 ctccagacgt tcgtcatatt ccgggcggcg cttaacacgt tcttcctcat cagggaataa
    17281 aaaggccaga cgctcttcca ggccctcttt tgcacgcttg accagataat cttcggtgct
    17341 catgtccccg gtcgggaact gcggcaggaa gtattcacca agacgcacag ttacgttaca
    17401 gcgtttggcg atctcaacgg tgttggcaag agcttcgggg atgtcggcaa acagctcgca
    17461 catctcctct tcgctacgca tatattgctg cggcgaatag ttacgcgggc gtttaggatc
    17521 gtcgagggta aagccgtcgt ggatcgcgac gcggatttcg tgtgcgtcaa agtcgctgct
    17581 gtcgataaag cgcacatcgt tagtcgccac gacgggcaaa ccgcgcgctt ccgccagttc
    17641 caccgccgcg tgcagatagc tttcttcatc cggcctgccg gtgcggatca gctcgagaaa
    17701 atagcgatcc gggaagtgtt cttcataaaa cgcgacacac tcatctacca gcgcgctgtt
    17761 accacgcaaa agactgcgtc cgacgtcgcc catgcgcccg ccggaaagaa ggatcaaccc
    17821 ttcgtttaat tcgataagcc agtcgcgatc gatgatcggc ccggcggcac cgtacccgcg
    17881 ctgatacgct tttgagatca gcaacgtcag attctgatag ccggtattgt tcgccgccag
    17941 taccgtcagg tgggttaact catcacccaa caggtcgcac tggacgttaa aatctgcccc
    18001 gacgataggc ttaatccctg cgccatgtcc cgctccgtag aacttcacca gaccgcaaag
    18061 gttggtgaaa tcggtgatcg ccagtgctgg catacccaac gccgccgcct tttttaccag
    18121 cggtgcggtt ttggccaggc catcgatcat cgagtagtcg ctgtgcaccc gcaggtgtac
    18181 gaaacgtggt tcagacatct tcagattccg gtttacttaa tctcgacaca agaatcagga
    18241 cgcaagtccc agtgctcgtt tgacaggccc aaagctgcga cgatgatgtt cagtcgcgcc
    18301 atattcagcc agtttttcca gatgaaaagc ggttggatac cctttgtgtt gggcaaaacc
    18361 atattgcggg aaaacaatat ccagcgccgc catttcggcg tcacgcgtca ctttcgccag
    18421 gatagacgcg gcactgattt ccggtacgcg gctatcgcct ttcaccacag ccatcgcagg
    18481 catcggtaat ttcgggcagc ggttaccatc aatcaacaca tattccggcg caatatgcag
    18541 cccagcgacg gcacgctgca tcgccagcat ggtcgcatga agaatgttca gctcgtcgat
    18601 ttcgtggggt tccgcgcggc ccagactcca gctcaacgct ttctctttga tctcttcata
    18661 gagcgccaga cggcgttttt cgctcagctt tttggaatca ttcagcccgg caatcgggcg
    18721 cgccgggtca aggatcaccg cagcggtgac gaccgcgcca actaacggcc cgcgtccgac
    18781 ttcatccaca cccgcaacca gctgcgtgtg cggatagaca aattcgatca ttgtgctaac
    18841 tccagaacgg cttgtgccgc ctgctcatcg gcattgcagc ggatctgctg atgcagttca
    18901 cggaaggtat cgtgcattgc gtggctggtt ttcccgttcg ccaacagcgg taacagcgcc
    18961 gcagccagtt tttgcggctc acactcttcc tgcaataatt ctttgactaa ctctctgccc
    19021 gccagcagat ttggcagcga gacatattca gttttcacca gccgcttcgc caaccagaag
    19081 gtaaaaggct tcatgcgata tcccaccacc atcgggcatt tcgccagcat acactccagg
    19141 gctgccgtcc ccgacgccag tagtgccgca tcgctggcga ccatcgcctc acggcccatc
    19201 ccatccagca aatgaactga aaggtctggc gcgacttcag ctttgatgcg ttcaaactgc
    19261 tcgcggcgtt tggcattcac cagcggcacc acgatctcca gatcggggta cgtctggcgc
    19321 aaaagctggg ccgttttcag gaaatcggcg ctcagcatct cgacttccgc accacggctg
    19381 cccggcaaca atgccagaca gtgggcatcg tgagggatac ccagcacatc acgggcggcg
    19441 tttttatctg gatctaacgg catggcatca gccatcgtgt gaccgataaa acggcacggc
    19501 acgttatact tgtcataaaa cgctttttcg aaaggcagaa atgcgagcac cagatcggtg
    19561 gctctgccta ttttgaaaac gcgtttctgt cgccacgccc agacggacgg actgacataa
    19621 tgaatggttt tgataccctg ctttttaagg ttaccttcga gggtaatatt gaagtcaggc
    19681 gcatcaatgc caacaaaaac atctggcttt agttcaccaa aacgctttgt cagatcggca
    19741 cgaatatgca gtaagcgacg cagacgaccg agcacttcaa caatgcccat caccgccaac
    19801 tcttccattt cgtaccaggc ttcgcagcct tcagcctgca ttcgtggccc ggcaacgcca
    19861 acaaagcggg cgttgggcac acgttctttc agagcgcgga ttaaaccggc ccccaggata
    19921 tcgccggagg tttctccggc gaccagggca atcgttaatg gacgctgttc agtcattaac
    19981 gaatcagacc gcgcgttgag cgtgcaaaga aatcggtaaa agctttcact tccggatacg
    20041 tttccgccag ttcagcaatt tccggtttca cttcatcgag cgttttaccg ctacgataaa
    20101 tcagcttata cgcattgcgg atagcggtaa tcgcctcgcg gctgaatccg cggcgcttca
    20161 gcccttcgat attgacaccg aacggcgttg cgtggttacc ctgcgcaatg acataaggag
    20221 ggacgtcctg cgccacaccg gagcaaccgc caaccatcac atgcgcacca atgatgcaga
    20281 actgatggac tgcggtcatg ccgccgatga tcgcgaagtc gtctaccgat acgtgacccg
    20341 ccagcgttgc gttgttggcg agaatacagc ggttacctac cgtacaatcg tgcgcaatgt
    20401 gcgcgttgat catcagtaag ttgtcgctgc ccaccttcgt caatccaccg ccctggactg
    20461 tgccacgatg aatggtgacg ctttcgcgaa tgcggttacg atcgccgatt tccacacggg
    20521 tcggttcgcc agcatatttc agatcctggt taacttcacc gatggaggcg aactgataaa
    20581 tctcattatc gcggccaatt ttagtatggc cattcacgac aacgtgagat ttcagtacgg
    20641 taccctcacc aatttcgaca tggggtccaa cgatacaaaa aggaccaatg tgtgcgttcg
    20701 cgccaatcga cgcgccctct tccacaatgg cggttggatg cacaaaggcg gatttatcaa
    20761 tcacgtatca ggcctcccgg ctacgagcac acatcatcgt tgcttcgcaa actactttac
    20821 cgtcgaccag agcaacccct ttaaaacggg tcaggccgcg gcgcgttttt tcgaaagtga
    20881 cttccatgat catttgatcg ccaggcacga ccgggcgctt gaagcgcgct tcgtcaatac
    20941 cagcgaaata gtacagctca cccggttcca gttttcctac gcttttaaac gccagaatac
    21001 ctgttgcctg tgccattgct tccagaatca gcacacccgg gaaaatcggt tttccaggga
    21061 aatggccctg gaagaatggc tcattgacag agacattttt tactgcgcgc agaaaacgac
    21121 cttcttcaaa atccagcacg cgatccacca gtaagaaagg gaaacggtgc ggcagaagtt
    21181 ctaaaatctc ttcaatctgc agagtatgag tgttagtagt caagatactc ttcctgtcaa
    21241 aatataagaa acgacaataa taacacggcc tgccgcaatc gtaagaatga gacaggccgt
    21301 aaagtttggc gaacaaaaga tggaacgtta gtcttgttga ttaaccttgc gctcaagcga
    21361 tttcagacgc ttgctcatgt catcaatgtt catcaccagt gcagcggttt tgcgccagac
    21421 tttgttgggt tgcagcggaa tgcctgagga atagacgcct ggttcagtga tgggacgcat
    21481 caccataccc atgcccgtaa ccgtcacttt gtcgcatatt tccatatgcc cgttgattac
    21541 gctggctccg ccgatcatgc agtaacgacc aattttcagg ctgcccgcca taatgacgcc
    21601 accggcaacc gccgtattgt cgccaatcac gacgttatgt gcaatctggc actggttatc
    21661 aatgatcacg ccattgccaa taatagtgtc atccagcgcg ccgcgatcga tggttgtgca
    21721 ggcaccgatc tccacgcgat cgccaataat tacgcgacca atctgtggga tcttcaccca
    21781 gttaccacga tcgttggcat aaccaaagcc gtctgcgcct accactgttc cggactggat
    21841 caggcaattc tgaccgatct ggatctcatg gtaaatggtt acgttcgccc agagacgcga
    21901 acctgcaccg attttgctgt ttttacctac gaagcaaccg gcaccgataa tcacgttatc
    21961 gcccagttca acgccggact caatcaccgc gttagcgcca atcgatacgt tgttacccag
    22021 cttcgccgtc gcgtcgatca ccgcactggg tgcaatgttc tgcgcgggct gcggcgtggt
    22081 atctaaaatt tgcgccatgc gcgcgtaagt caggtaggga ttcttcacta ccagcgcggc
    22141 acttttcgcg aaaggaagat cgtcctgggt catgacaacc gcggacgcct ggcacaagcc
    22201 taaatgctca cggtattttg ggttaaccat gaacgtaatg tgacctgttt gtgcagattg
    22261 catggacgca acgccggtga tgacgatatc gccatcaccg tgtagttctg catccaactg
    22321 ctgcgctaaa tcagccagtc gaattgaagg cattacttat ttaacctgtt tcagtacgtc
    22381 ggcagtgatg tcttttacat cgctgctgtt gtaagcaacg gcgtttgcat caacaaccag
    22441 atcgatatcc tggctgttgg caacggattt cacagcagtc tggatacgag taaccagttt
    22501 gccgcgttct tcgttggaac gacgtgcgcg atcctgctca aaagcctgcg ctttctgagc
    22561 aaaagtctgg cgctgagcca tcacgtcttt ttccagctta gtgcgatcgc tgcccgcttt
    22621 catggactgc agctttttca ttttagcctg cagatcggtt tccatacgct gcagttcgct
    22681 ggcacggcct ttgaactcat tttccagcgt gttagaaaca ccggttttct gcgctacctg
    22741 ctggaacagg ctgcccatgt tgacgattgc aattttgtca gccgcctgag cagatgttgc
    22801 cagtgctaaa ccgagacctg cagctaataa ccactttttc acaataaact ccttaccatc
    22861 ccatttgcac cggagggtgc agttctttgc gtggcccggc gatcttatat tgatcgccta
    22921 aagtcatcgc tacactacca ctacattcct ttgtggagaa cacttaccag gttttaccga
    22981 tgttaaactg gaactgttct gccttgtctc catcgtactt tttgaacggc tgggcgtagg
    23041 agaacaccaa cggccccaat ggggacatcc attgtaatgc gatacccgca gacatacgga
    23101 tattgcttgg atcactatag tccggatatc cagaatattg gctggaatcc cagtttgtat
    23161 cccaaacggt acccatatcc cagaagaagg aagtacgaac cgagttagca tacttatcgc
    23221 taataaacgg cgtcggggtg atgaactcga ggctggcaac cgccatggcg ttaccgccta
    23281 cagcatcatc cgatttacac aggtctttcg cgccgtcctg agtcgcacat tcgtaatcat
    23341 agtccggatc ataattactg gcctgatgcg ggaagtaaac tgctttcgga ccaatggtat
    23401 tggactggaa gccacgcacg gtgctggaac caccggcata gaagttctcg tagaacggca
    23461 tctctttgcc gcctaaacca tcaccataac cccagcgggt acgccccaga acaacccatt
    23521 tgtgatcgtc atcgatcggc acataagtcg ccgtgtctaa cgtcactttg tagtattcgt
    23581 tatccgatcc aggaatggtc actttaccgg tcaggttgac acgtgaacca tctgtcggga
    23641 agtaaccacg gtcaagcttg ttataggtcc aaccatagtt gaacgtgaag tcgtccgttt
    23701 tgaagctgtt atcctgatca gaggtgctcg gatgttcacc catagagtac agataacgcc
    23761 acatcgcaac ctgaggctgc atgttggaca gggagttatg tacataaccc agacctgcac
    23821 gcagcgagtt atattcgttg atcgggaagc ccaacgtcac gtctgtacca taactcttgt
    23881 tggtatagtc ggacaggtcg gcgtcatctg cctggaagtc attatagaag agacgaccac
    23941 cgaggcttac accatctaca gtgaagtacg ggttggttac cgacaattca gcataggtct
    24001 ggtaatcgtt tttggtcccg ttgataccaa cagcataacc tgtacctaac cagttatcct
    24061 gttgcacgcc agcctggaag ctcacgccac tttcagtacc gtaaccaatc ccgaagttga
    24121 agctaccggt gttacgttct ttcaccttgt agactacatc tacctggtcc gggctacccg
    24181 gaacacgttg ggtatcggta tcgacagttt caaagaagcc cagacgattc agacgctcct
    24241 taccctgatc gaccagatcg cttcccagcc atgcaccttc catctgacgc atttcgcgac
    24301 gcaggacggc atctttcgag gtatcgttac cttcaaaacg gatcttacgc acgtagaaac
    24361 ggttacccgc atcaacgttc acgcgtaatt taacggtttt gtcggcatcg ttaatttcag
    24421 gcatcgactg tacgcgcgga taggcataac catagcgacc gagaagcttt ttgatgtcat
    24481 cttccatctt ggtcactttg gtgccgttat acagctcgcc cggctcgatc ttagtcagct
    24541 gctcaatttc agcggagtgc ccggcaaggt tgccgctcac ttgaacgcca gaaagcttgt
    24601 actgatcgcc ttcggtgatg ttcaccgtga cgtaaatacc ttttttatct ggcgtcagac
    24661 tgacctgggt agagtcgatg ttgaaacggg cataaccgcg atccagatag taactacgca
    24721 gggtttcaag gtcgcccgcc agtttctgtt tctggtattt acgatcgcct accacgttcc
    24781 accacggcac ttcgtcacgc agttggaaat gagagatcag ttcgtcggtg gtgaaagcat
    24841 ggttaccaac aatgttaatt tgctggattt cagctgacac accttcctgg aacaccagtt
    24901 ttaggtcaac acggttgcgc ggcagcgggg tcacgacagc ttttacgctg gcgctatatt
    24961 taccgacgct gtagtagaag tcttccagac ctttctcgat atcggcaatg gtggtgcgat
    25021 cgagggattc gcccacacgc acaccagaag cctcgaggtt ttgcttcagc atgtcatctt
    25081 tcaccgattt gttaccggag aaagtaatgc tggcaatggt cggacgttct tttacctgaa
    25141 ccagaagggt atcaccatca cgaaggacgc gaacatcctc aaagttgccg gtagcaaaca
    25201 gagcgcgaat ggtattactg atatcttcat cattaaccgt gtcgcctgtg cgcaccggca
    25261 tactgaggag ggccgcacca acggcgacac gctgaaggcc ttcgaaatga atatctttca
    25321 ctacgaaccc ttcagcaccg tatacggtgg cgctgctaaa cagcagcgac gctatgagca
    25381 actttttcat cgccatcgtt attatgcgtt cttcctaact aactctcata accgagagaa
    25441 atcattgaaa agtgcaagcc ccattaacag caccagcaga atcgagccaa tgcgataaca
    25501 aaagtcttga acccgctcgg ataccggtcc gcccttgatc ttttcgatcg caaggaacag
    25561 cagatgcccc ccgtcaagta cgggcaacgg aaacaggtta attatcccta agttcacgct
    25621 aataagcgca agaaacggca ggtaataaac taccccgagt tccgctgtca tcccagcccc
    25681 cttggcgata gagatcggcc cactgaggtt gttcagtttc acatcaccgg tgatcaattt
    25741 tcccagcata ctgaccgtca gcttcatcag ctgccacgtt ttgtccgtgg cttcgacgat
    25801 ggcgttgaac ggcccatact ggcgtacaac tttatactca tctggcaaag gaatgacttt
    25861 cggctcaata ccgacaaaac caatcgcttt accattaccc ggtttactct ccgggattaa
    25921 tgtcaaagac aagggactcc cctgcctttc gatttctaac gctaaggatt tacccgggtt
    25981 atcccggaca agcatcacaa aggtcaccca ctgcgttaag ggctgaccat cgactttaac
    26041 gatcctgtcg cctgcttgca aacctgcctt gcttgccgcc gagtttggct gcacattttc
    26101 cagtacaggt tcaatttgcg gcccacgagg acgaatcccc agcgaagata ccggatcttc
    26161 tttatcaggc tcaaacgccc agtgacgtaa atcgagcttt acatcccgcc gttggtcgct
    26221 gccaaatggc gctactgtaa tggtggtgct ttcatcgcca attttatcga ccaactgcaa
    26281 acgcacggca tcccaatcag gcgtttcgat accatctacg gcttttagtt ccgtacctgg
    26341 tgcaatttgt gcttccgcag ctatcgaatt ggctgctatt tcgccaacca ccggacgtac
    26401 gccaggcaca ccaataataa aaaccagcca gtaggcaaag atagcaaaaa tgaagtttgc
    26461 aaccggacct gcggcaataa tcgccgctcg ttggccgaca gatttattat tgaaggcatg
    26521 gtggcggagt tccggaacga ccggttctgc gcgctcatcc agcattttga cataaccgcc
    26581 caacgggatc agggcgataa catattcggt gccgagctta tcagttcgcc gccagagcgc
    26641 cttaccaaac cctattgaga aacgctcaac gcgaacacca caacgccggg caacccagaa
    26701 atgaccaaat tcatgcacgg tgataagtac acccagtgca acgatgaacg aagccaaatc
    26761 ccagagaaaa ctcagcatat taccttccgt taaagcgtcc tgaataccag taacaacaag
    26821 caagcaaaga ccggtaccgc agccgtcagg ctatcaatac gatctaaaat accaccgtgt
    26881 cctggaatta aatgaccgct gtccttaatt cctgcttcac gcttaaacat actctcggtc
    26941 agatcgccga gcactgaggc taacgctgcg acaatagagc aaatgagtaa ggtgacggga
    27001 gcgacgtcga gattcgccca catgccataa ccccatgaga ttactgctgc ggtcgcgagt
    27061 ccacctataa agccttgcca ggttttaccc ggagaaacct tcggtgccag cttatgttta
    27121 ccaaacaatt tgccaaacat atatgcgcca gagtcagcgc cccataccag gatcatgaca
    27181 tagagcagcc atattgcgcc actgtaatga ttctcgtcgt agtgccaggc ccgtaacgcc
    27241 agcatgcccc agaagaaggg aacaatggtt agcacgccaa aaataaggcg caatgtttta
    27301 gagttacgcc agattgctgc ggaacctggg taaaacagca ccagcaatag cgcgacaatc
    27361 caccaaccca gcgaagccca aagtgagatt tcaaccagcg gttgatgaat atttcggtga
    27421 tattccggca acagaaaaag catcagcgcc aacaataacc cgcataacac cgccaaccat
    27481 actcgctgcg aacgagtggt aaaaccgcta agctgtcccc attcccacgc tgccagcatg
    27541 cagaccacca gcgttacaat ggcgaacccc accggcggca acagaaacaa cgccgcgatg
    27601 acgacgggta ttaacacaaa agcagatatc aggcgatact tcagcaaaag cgacccccat
    27661 caggctgttt catcaccggg ctcggtgccg ccgaaacgac gctctcgatt agcaaaggca
    27721 tttaacgccc cttcaaagtc ttgttcatcg aaatcgggcc agagaacatc tgtaaagtaa
    27781 agttcggcat aggcaatttg ccaaagcaaa aagttactaa tgcgatgctc ccccccagtc
    27841 ctaattacta aatctacagg ggccagttca tgcatacaga catgctggtt tagcatctct
    27901 tcatctatct gatctggttg caggtttcct tgctgcacct tttcagccag ttgcctgact
    27961 ccctggacta tatcccaacg tccaccgtag ttcgccgcaa tattcagcgt cagaccggta
    28021 ttcccggctg ttagcgcttc agatttacga atacgttctt gcaaacgcga gttaaagcga
    28081 ctggtatcgc caataatacg cagacgcacg ttatgtcggt gcagactttt tacttcgcta
    28141 tcgagcgccc acacaaacag ttccattaac gcactgactt cctgcgctgg tcggttccag
    28201 ttttcactac taaaggcata cagcgttaac gcctcaatac cgttgttggc cgcaaaagag
    28261 acagcccggc ggacggattt tgccccggct ttatgcccaa aggcacgaat cttcccttgc
    28321 ttttttgccc agcgaccatt gccgtccata atgatcgcaa catgacggca gccatgcgct
    28381 ggcaattttt cgctaagtgg ttgagtagca gacaacatca cgcgtttttt attccctgac
    28441 agggtttacg gtactcagga atactgaagc ctatacaaaa aaaagccgtg taaaaccacg
    28501 gcatacctga ccttataaag ccaactactt acgatcagat ggcgcagact atatcactga
    28561 agccctacgc taacaaatag cgcgactctc tgtagccgga ttatcctcag cttgcgagac
    28621 gcatcacctc ttttctggcg acttcacgcg cgttcgcatc aacagataac acatcgtcca
    28681 cacattgtgg ttcgcgcata tccatttttt ccagtacgga taaattcaac gcagcgatat
    28741 ccgtaaagcg gatttgttgc gcaagaaaag cagcaacggt gatttcgttt gcggcattca
    28801 atgctgtcgt cgctgcctgg ccttgttcga acgcctccat cgccagtttc aggcatggat
    28861 aacgatcata atccggtgcg gcaaatgtca acgcacttag tttgcaaaaa tcgagcggct
    28921 tcacgccaga gttcacgcga ttcggccatg ccatggtgtg ggcaattggc gtacgcatat
    28981 ccggttcccc cagctgcgcc agaacactgc cgtcctgata gcgcaccatt gagtgaatca
    29041 ctgactgcgg gtgaatcagc acttccatct ggctggcgct ggcgttaaac agccaacgcg
    29101 cttcaatgta ttccagacct ttgttcatca tggtagccga atcgacagaa attttacgtc
    29161 ccatcgacca gttcggatga cggcaggctt gatcgggcgt cattgttgcc aaatcgcgca
    29221 atggcgtctc acggaaaggg ccaccagacc cggtaagtaa aatggacacc acgccatttt
    29281 gctcaaggtc agcgtatccc agattatgct ggataggttg cggtaaactc tgaaaaatgg
    29341 cgttatgttc gctatcgacc ggtaacaatt gcgctttgct ctgctttacg gcgtccataa
    29401 acagacgtcc gcaggtaacc agtgattctt tattggccag caaaatggtt ttacccgcgc
    29461 ggatcgcagc aagcgtaggt aacagcccag cagcgccaac aatggctgcc atcacctgat
    29521 caacatcctc aagcgctgcc atatcgcaag cggcttgttg cccacttaag acttcggtgc
    29581 ggctaccctg ttgctgtagc atcgttttaa gaagtttcgc actcgcttca tcgtccatta
    29641 cggcatagcg gggagagaat tccaggcact gttctaccat gcgagtgaca tttttgcctg
    29701 ccaccagcgc aactacgcgg aagtgttcgg gattatggcg caccacgtcc agcgtgctgc
    29761 aaccaatcga gccggtcgag cccagaatgg tgagttgctt catgaaacat ccagagttga
    29821 gacagaataa aaagcaaaac gccgccagcc gatccgcaag gatctactga gcggcgtttt
    29881 tgtcgttcaa gaaatcagaa ctgcatcagt tctgcttctt tgtctgccag cgccgcttca
    29941 attttcttga ttgcagcatc agtcagtttc tgtacatcgt cctgagaacg gcgatcgtcg
    30001 tcttcgctga tctctttatc tttcaacagt gctttcactt tgtcgttcgc gtcacgacgc
    30061 acgttacgta ctgcaacacg cgcttgttct gcttcaccac gaacgatttt ggtcagatct
    30121 ttacgacgtt cttccgtcag cggcggcagc ggaacacgga tgtcgctacc cgcagagttc
    30181 gggttcaggc caagatcgga cgccataatc gctttttcaa cggccggaga cattgaacga
    30241 tcaaacacgt tgattttcag agtacgggaa tcttctaccg ttacgcttgc cagctgacgc
    30301 agcggcgtcg gcgtgccgta atattccacg acaatgccat ccagcaggct gggagaagca
    30361 cgacccgtgc gtattttgct gatttgggtt ttgaacgctt ctacgcattt gtccatgcgt
    30421 acttcagcat cttttctgat atcgctaatc acgttacgaa tccttgaaaa cttgtctcag
    30481 tcagaccaga cagtcacaca cagtggaagt gtgctaagta tagccctgat taaacatatt
    30541 acgggtaaga tcccccgcga tacttacgcg gaatcttacc cttatttatc catcacggga
    30601 attattccgt gattaaagtc ccttcttttt cacccattac cacacggcgc agcgcacccg
    30661 gtttgttcat gttgaaaaca cgaatcggta atttatggtc acgagccagc gtgaaggccg
    30721 ccaggtccat gactttcagc tctttttcca gcacttcgct gtaagtcaat tgctcgtaca
    30781 tggttgcagt tggatctttc gccggatcag cagtaaacac gccgtcaact ttggttgctt
    30841 tcagcaccac atcggcttca atttcgatac cacgcaggca agctgctgag tcggtggtaa
    30901 agaacgggtt acctgtaccg gcggagagga tcaccacacg gttgttgcgc aacaggctga
    30961 tagcttctgc ccagctgtag ctgtcgcaca cgccattcaa tggaatagcg gacatcaggc
    31021 gagcgttcac ataggcgcgg tgcagtgcat cacgcattgc caggccgttc attacggtcg
    31081 ccagcatccc catgtggtcg cccacaacgc ggttcatacc cgctttcgcc agaccagcgc
    31141 cacggaacag gttaccccca ccaatcacca caccaacctg aatacccagt tcaaccagtt
    31201 ctttgatttc ctgagccatg cgatccagta tgcttgcatc aataccgaag ccttcagtgc
    31261 cctgcagagc ttcgccactc aacttaagca gaatgcgttt atagacgggt tttgcattgg
    31321 tagccatgtt tctttcctga gactgtcaac gattgaaatg aattaatact agcgacattg
    31381 tacgtcgctt ttcagcgcag ccccacaagg gttagctgaa tttacaagat gggcacaaaa
    31441 agaagccgcc ctcaggcggc tccttttcga taattaagac tgcttggaca tcgcagcaac
    31501 ttctgctgca aagtcagtct caactttctc gatgccttca cctacttcga agcggatgaa
    31561 gccagtcact tcagcgttat gctctttcag cagctgacca acagttttgc ttggttccat
    31621 aacgaacggc tgaccggtca gagaaacttc gccggtgaat ttcttcatgc ggccttcaac
    31681 cattttctct gcgatttctt tcggcttacc agactgcatc gcgatatcca gctgtacctg
    31741 gtattctttc tctaccactt cagcggatac gtcttccggt ttgatgaatt ctggcttgct
    31801 tgcagcaacg tgcatagcga tgtgtttaac cagctcttcg tcagcgcctt tagcagcaac
    31861 cagaacgccg atacgcgcac cgtgctgata agaacccaga acgtcgcctt ccagcgcagc
    31921 aacgcggcga atgttgatgt tttcaccaat tttcgctacc agcgcaacac gttcttcttc
    31981 gaactgtgct ttcagaactt caacgtcagt gattttgcca gcaacagctg cgtccagaac
    32041 tttgtctgcg aacgcctgga aaccagagtc ttttgcaacg aagtcagtct ggcagttaac
    32101 ttccagaatg atgccgtagt tgccgtcgat tttggttttg atcacgccgt cagcagcaac
    32161 gttgcctgct tttttcgctg ctttaatagc accggactta cgcatgtttt cgattgccag
    32221 ctcgatgtcg ccgttagctt cagtcagtgc ttttttgcaa tccatcatgc ctgcgccagt
    32281 acgctcacgc agctctttta ccagggatgc ggtaatttca gccattctaa aatcctcgga
    32341 gatgtgatct gcccggcatt gaaccgcaca gatataaaag tgaaaaaggg ggccatatgc
    32401 aggcccccta accaaacgtg tactacctgg tctataaggg ctcgtacgag cgtgccttat
    32461 tactcagctt ctacgaagct ttcttccgcc tgggaagcca gatcctgaga acggccttca
    32521 cgtacggttg cagcaacagc gcccaggtac agggtcacag cacggattgc gtcgtcgtta
    32581 cccgggataa cgaagtcaac accgtccgga tcagagttgg tatcaacgat agcaaatacc
    32641 ggaataccca ggttgtttgc ttctttgata gcaatgtgtt cgtggtcagc atcgattaca
    32701 aacagagcgt ccggcagacc gcccatgtct ttgataccgc ccaggctgtt ttccagtttc
    32761 tccagctcac gagtgcgcat cagcgcttct ttcttggtca gcttgtcgaa agtaccgtcc
    32821 tgagactgag tttccaggtc tttcagacgt ttgatggact gacgaacggt tttccagtta
    32881 gtcagcatac cgcccagcca gcgatggttc acgaagaact ggtcgcagct cagagcagcg
    32941 tctttcaccg cttcgcttgc agcgcgttta gtaccaacga aaaggatttt acctttgcga
    33001 gaagcaatct tgttcagttc agccagagct tcgttgaaca tcggtacagt tttctcaagg
    33061 ttgatgatgt gaactttgtt acgcgcaccg aagatgaacg gcttcatttt cgggttccag
    33121 taacgggtct ggtgaccgaa gtgaacacca gccttgagca tgtcgcgcat ggaaacagtt
    33181 gccatgatta aaacctctat ataaaagttg gggttatgcc tccacgtatc ccatattacc
    33241 gaccccaaag ggcaccccgg aatatgtgcc gatacgtgtg tgttgttaca caaagtgaga
    33301 ttgtcgcttc cgtccagtct gtgtatacga aatggatcgg aagtccggcg cgctttatac
    33361 cacaaatacg ccgtggacac caataattgt tggcgctgtg tacagcatca gacgtcgaat
    33421 tttctattat agaaaacctt cagtggcacg tttggcgaaa ttcagaatga ttctcaattt
    33481 gccagggtgt gataccattg acggcactta catatatatt gtcggtatca ccgacgctga
    33541 tggacagaat taatggctat ctcaatcaag accccagaag atatcgaaaa aatgcgcgtc
    33601 gctggccgac tggctgccga agtgctggag atgatcgaac cgtatgttaa accgggcgtc
    33661 agcaccggcg agctggatcg catctgtaat gattacattg ttaatgaaca acacgcggtt
    33721 tctgcctgcc tcggctatca cggttatccg aaatccgttt gcatctcaat taatgaagtg
    33781 gtgtgccacg gtatcccgga cgatgcgaag ctgctgaaag atggcgatat cgttaacatt
    33841 gatgtcaccg taatcaaaga tggtttccac ggcgatacct cgaaaatgtt tatcgtcggt
    33901 aagccgacca tcatgggcga acgtctgtgc cgtatcacgc aagaaagcct gtacctggcg
    33961 ctacgcatgg taaaaccagg cattaatctg cgcgaaatcg gtgcggcgat tcagaaattt
    34021 gtcgaagcag aaggcttctc cgtcgttcgt gaatattgcg gacacggtat tggtcgcggc
    34081 ttccacgaag aaccgcaggt gctgcactat gactcccgtg aaaccaacgt cgtactgaaa
    34141 cctgggatga cgttcaccat cgagccaatg gtcaacgcgg gtaaaaaaga gatccgcacc
    34201 atgaaagatg gctggacggt aaaaaccaaa gatcgcagct tgtctgcaca atatgagcat
    34261 actattgtgg tgactgataa cggctgcgaa attctgacac tacgcaagga tgacaccatc
    34321 ccggcgataa tctcgcacga cgaataaaat gaagccggcg aatgccggct tttttaatgc
    34381 gataatttaa tcttatgggt ggcgcacaat gaataccctt ccagaacagt acgcaaacac
    34441 cgctctcccc accctgcccg gtcaaccgca aaatccatgc gtctggcccc gtgatgaatt
    34501 aaccgtcggt gggataaaag cccatatcga tactttccag cgttggctgg gtgatgcctt
    34561 tgacaatggg atctctgcag aacagttgat tgaggcgcgc accgagttta tcgaccagct
    34621 cctgcaacga ttatggattg aagcgggatt cagccagatt gccgacctgg cattggtcgc
    34681 cgtcggtggc tacggtcgtg gcgagctgca tccactttct gacatcgatt tactgatttt
    34741 aagccgtaaa aagctcccgg acgatcaggc gcaaaaagtg ggcgagctgt taacgctgct
    34801 ctgggatgta aagctggaag tcggtcatag tgtgcgcacg cttgaagagt gcatgctgga
    34861 agggttatcg gatttaaccg tcgccaccaa tttgatcgaa tcccgcttat taattggcga
    34921 tgttgcgcta tttcttgaac tgcaaaaaca tattttcagc gaaggattct ggccttccga
    34981 caagttctac gcggcgaaag ttgaagaaca gaaccagcgc catcagcgtt accacggcac
    35041 cagctacaat cttgaaccag acatcaaaag tagccctggc ggcttgcgcg atatccacac
    35101 tctgcaatgg gtggcccgcc gtcattttgg cgcaacatcg ctggatgaaa tggttgggtt
    35161 tggcttctta acctcagcgg agcgggcgga attaaacgaa tgtctgcata tattgtggcg
    35221 tattcgcttt gccctgcatc tggtcgtcag ccgttacgat aatcgcctgt tattcgatcg
    35281 ccagcttagc gtcgcccagc gtctgaatta cagtggcgaa ggtaacgaac cggtcgagcg
    35341 gatgatgaag gattacttcc gcgtgacacg ccgcgtcagt gaactcaacc agatgctgct
    35401 gcaactgttc gatgaagcca tcctcgccct tcccgccgac gaaaaaccac gtccaatcga
    35461 cgatgagttt cagctacgcg gtacgctaat cgacctgcgt gatgaaacac tatttatgcg
    35521 ccagccggaa gccatcttgc gtatgttcta caccatggtg cgcaacagtg cgatcaccgg
    35581 catttactcc accacactgc gccagttacg ccatgcccgt cgccatctgc aacaaccgct
    35641 gtgtaatatt ccggaagcac gaaaactgtt tttgagcatt ctgcgtcacc ccggagcggt
    35701 gcggcgcggg ctattgccaa tgcatcgcca tagcgtactc ggcgcgtata tgccgcaatg
    35761 gtcacatatc gtcgggcaga tgcagtttga tctgttccac gcctacacgg tggatgaaca
    35821 tactatccgc gtgatgctga aactggagag ttttgccagt gaagaaacgc gccagcgcca
    35881 tccgttgtgt gtggacgtct ggccgcgcct accgtcaact gagctgattt tcatcgccgc
    35941 gctgtttcac gatatcgcca aaggacgcgg cggcgaccat tccattctcg gtgctcagga
    36001 tgtagtgcat tttgccgaac tccacgggct gaactcacgc gaaacacagc tggtcgcctg
    36061 gctggttcgc caacacttgc tgatgtcggt gacagctcag cgtcgcgata ttcaggaccc
    36121 ggaagtcatc aagcagtttg ccgaagaagt tcaaacggaa aatcgtctgc gctatctggt
    36181 atgcctgaca gtggctgaca tttgcgccac caacgaaacg ctgtggaata gctggaagca
    36241 aagtctgttg cgcgaactct atttcgccac cgaaaaacag ctacgacgcg gaatgcaaaa
    36301 cacgccggat atgcgcgaac gggttcgcca tcaccaactc caggcgctgg cactgctgcg
    36361 catggataac atcgacgaag aggcgctgca ccaaatttgg tcacgctgtc gtgctaacta
    36421 ttttgtccgc catagcccaa atcaactggc ctggcatgcg cgccatttat tacagcatga
    36481 tttaagcaaa ccgctggtat tgcttagccc gcaggctacg cgtggaggca ccgagatttt
    36541 tatctggagc ccggaccgcc cttacttgtt tgctgctgtt tgtgccgagt tagatagacg
    36601 caacttaagc gttcacgacg cacaaatatt caccactcgc gacggtatgg cgatggatac
    36661 ctttatcgtg ctggaacccg atggcagccc gctgtccgca gatcgccatg aggttattcg
    36721 gtttggtctg gagcaagtac tgacacaaag tagctggcag ccaccgcagc cccgtcgcca
    36781 acccgccaaa ttgcgccatt ttactgttga aaccgaagtg acgtttttgc caacccatac
    36841 cgaccgcaaa tcgttcctcg aactgatcgc cctcgaccaa cctgggctgc tggcgcgagt
    36901 cgggaaaatt tttgccgatc tgggaatttc gcttcatggt gcccgaatta caaccattgg
    36961 cgagcgagta gaagatttat tcataattgc caccgctgac cggcgtgcac ttaataacga
    37021 gttgcagcag gaagtgcatc agcggttgac agaggccctc aatccaaacg ataaagggtg
    37081 atgtgtttac tgatatgaaa agagtttaac aatgcagcag ttacagaaca ttattgaaac
    37141 cgcttttgaa cgccgtgccg agatcacgcc agccaatgca gacaccgtta cccgcgaagc
    37201 ggtaaatcag gtgatcgccc tgctggattc cggcgcactg cgtgtagcgg aaaaaattga
    37261 cggtcagtgg gtgacgcatc agtggttgaa aaaagcggtg ctgctctctt tccgtattaa
    37321 tgataatcag gtgatcgaag gggcagaaag ccgctacttc gacaaagtgc cgatgaaatt
    37381 cgccgactac gacgaagcac gtttccagaa agaaggcttc cgcgttgtac cacctgcggc
    37441 ggtacgtcag ggtgcgttta ttgcccgtaa caccgtgctg atgccgtctt acgtcaacat
    37501 cggcgcatat gttgatgaag gcaccatggt cgatacctgg gcgaccgtcg gttcttgtgc
    37561 gcagattggt aaaaacgtcc acctttccgg tggcgtgggc atcggcggtg tgctggaacc
    37621 gctgcaggct aacccgacca ttattgaaga taactgcttc atcggcgcgc gttcagaaat
    37681 cgttgaaggc gtcatcgtag aagaaggttc ggtgatctca atgggtgttt acattgggca
    37741 aagcaccaag atctatgatc gcgaaactgg cgaagtccac tacggacgcg ttccggcggg
    37801 gtctgtggtt gtttcaggca atctgccgtc gaaagatggc aaatacagtc tttactgtgc
    37861 ggttatcgtt aagaaagttg acgcgaaaac tcgcggcaaa gtcggcatta acgaactgtt
    37921 gcgtaccatc gactaaaagt atgcacacgg gcagcacgac gctgcccgat tgctttctgg
    37981 ttcagaataa ttcgtcctac tcattcttcc tccccctttt ttaaatactt tttcatcttg
    38041 tttcggactg tcatgtaatt cgcaaaacga agataaatgt gctaatccat cccatggaat
    38101 atcgccacaa taaagcaggt aattttttga tttttacgcc atgatagaag tctggaagaa
    38161 aaaggctttc tgcgatcaca gtcgagccgg gcgggccagg ccaaatgcta aaataagcac
    38221 aatttactga ctcattttga ggtaacgcta tgtacgacaa tctgaaaagt ctgggtatta
    38281 ccaatcctga agaaattgat cgttacagcc tccggcagga agccaacaac gatattctga
    38341 aaatctattt ccagaaagac aaaggcgagt ttttcgccaa gagcgttaag tttaaatatc
    38401 cgcgtcagcg taaaacggtc gtcgctgatg gtgtgggtca gggttataaa gaagtccagg
    38461 aaatcagccc gaatctacgg tatatcattg atgagcttga tcaaatctgt cagcgtgacc
    38521 gcagcgaagt tgatcttaag cgtaagatcc tcgacgactt acgtcacctg gagtcagtcg
    38581 taaccaataa gatcagcgag attgaagccg atctggaaaa actaacgcgt aaataatccc
    38641 gaattgacgg ttaactcggg cattcccctc tccccttcgg gctgagggga atgagccagc
    38701 gcctcctcaa ccttaacgca taacctaccg ctcttcatcc agttgtaacg ccacatacag
    38761 cagcaacctg tcatcaaaat tgcccaaatc aagcccggtc agttccgata tacgattaag
    38821 ccgatactcc agggtattac gatgaataaa caacgccttt gacgttgcca gcggttgcac
    38881 attgtgacga aaccacgccg ccagcgttcg tcgcagcaag ccgttattgt ccatcgcttt
    38941 cagccgcgcc agcggtcgcg ccagttcgtt ggcctgccag tcgccacgca aactgtcgag
    39001 taacacaggt aacatcagat cctgataaaa atagcagcga ctttctggca tccgctgttt
    39061 acccaccacc atcgtcgttt tcgccgtacg ataggatcgg gcaatactgc caggaccggt
    39121 aaaatagttg cccagtgaaa cgcgaaaacg cagctggccg tactctttca tgcgggtaat
    39181 cagttgttca actcgcttac gatgatcttc tgcatcccag cgcccaaaag agttcaacgc
    39241 cggtttcaac accaccattt cggttagcga gacaatcgcc accagattat tacgttcggg
    39301 cgtagtcagc gcgttttgca gttgttgtaa ctccgccatt gcgctgtcca caccaagctg
    39361 accgctgtcg acctcaacaa tagccaccac tcgcggttga ttgagatcga tccccagccg
    39421 ttgcgcccat tcagtaagtg cgggagtatt ctcctctgcc tgaatcaggt tcatcaccag
    39481 ttcctcccgc aaacggctat cctgcgccaa caagtgcatc aaccgcgact gttccagcat
    39541 catttcagcc gtcatgcaga ccagttcgcc atatttacgc agattctctg gttcacctgt
    39601 caggccaatt acgccgacaa tttcaccttc cagccgtaac ggtagattaa tcccctgccg
    39661 cacaccgtgc agatgacgtg ccaccgcgtc atcaatatcg acgactcgtc cctgtgaaag
    39721 taccagcaat gctccttcgt gcaattcacc aatacgctca cgatcgccgc tgccgataat
    39781 tcgcccgcgg gcatccatta cgttgatatt ggtatcgatg atgcgcatgg tacgtgccac
    39841 gatatcctgc gccattttgg tatcaagatg ccagccagcc ataagtcctc cgttatgcac
    39901 ggcttagcat aaggaagtac gtaacgtacg gcattgtgca aatgcctaaa ggatgatgaa
    39961 gatgtatgga gttgtggtga agttcacaga ttgtaaggag aaccccttcc cgttttcagg
    40021 aaggggttga gggagattac tgcattaaca ggtagatggt gctgtcgccg cgctgaatgt
    40081 tcagtgccag cacagacggt ttgctgtcga gaactttacg cagttcagcg atgtttttca
    40141 ctgcctgctg gttcgcgcca ataatcacat cacctttctt caggccgatc tgcgcagccg
    40201 gagtgcccgt tttcacgttg ttcactacca cgccctgatc tttgcctttg ttgctcatct
    40261 cagcgccttc aatgccgttg aagatggagc tggaatcaac ctgattctgg ctgctctgct
    40321 gcagttccag attcacgttg acctgcttac cgtcgcgcag caagcccagg gtcagtttgc
    40381 tgcccaccgg catagtaccc acttgagcac gcagtgcggc aaagctgctg attggcttac
    40441 cgttcagtga ggtgatcaca tcacctgctt taatgcccgc ttttgcagcg gaggagttag
    40501 gcagaacctg gcttacgaaa gcaccgcgct gggcgtcaac tttcatcgct ttcgccagtt
    40561 cggagttcag ctcagtcccc ataataccca gctcaccgcg tttcacctgg ccgtattcca
    40621 ccatctgcga ggtcaggttt ttcaccatgt tactcgggat agcaaaaccg ataccgatgt
    40681 tgccgccgtc cggtgcgagg atcgcggtgt tgataccgat cagttcgccg ttcaggttaa
    40741 ccagcgcacc accggagtta ccacggttga tcgctgcatc ggtctggatg aagttttcgt
    40801 agttttcggc attcaggccg ctacgcccca acgcagagac aatcccggaa gttaccgtct
    40861 cgcccaaacc aaacgggtta ccaatcgcta cggtgtaatc acccacgcgc agtgcatcag
    40921 aatccgccat cttaattgcg gtcaggtttt tcgggttctg gatctggatc agcgcgatat
    40981 cagagcgcgg atctttgcca accatcttcg cgtcgaactt acggccatcg ctcagttgaa
    41041 ctttaatgac cgtcgcgtta tcaacaacgt ggttgttggt gacgacatag cctttatcgg
    41101 catcaatgat gacgccggaa cccagcgcca tgaatttctg ttgctggccg ccgccattac
    41161 cgccctggcc cccctggcag aacggagagc tctggaacgg agaaccttcc tggcagaacg
    41221 gagaatcatc accgaagaac tgctggaaat tacgcggcat acgcggcgta ttaacggttg
    41281 tgctaccttc tacgttaatg ctgaccactg aaggcatcac cttttcgagc atcggtgcaa
    41341 ggcttggcat ctgctgggct gtcgttgctg aagaagtctc agccgccgtt gcagagagcg
    41401 gagataacgc caaacctaaa ctcagagcca gtgcactcag tgctaatgtg gtttttttca
    41461 tgtatttcag tctcgattaa cagataacgc aaaattgctg tgttcttcag attcgtttta
    41521 tagcgcgaag ttccgaacta aagtttctgc aaaaggtaaa aatttattgt tcgtctttac
    41581 aaaagcctgg ttattgttct acggccatca gacgtcggta ttcatcccac gcatagaggt
    41641 cggtcatacc gctgatataa tcctgcagca ggcggcaacg gtaataatat tcccatagcg
    41701 gaaactcagg agaatctgac ggtaatttac tgacagcttc gacataggcc agccgatggc
    41761 gcgtcgagag tttgtggaat aagcgcgatt caatagggaa acgtttcacc cgttcttttt
    41821 ctaccagttc agtaaagtct gataacgaca ggcttaataa agggcgataa atctctaata
    41881 atccgctaat gacccgatag ccctgcaatt caagctgctc gacatctgga tggctaaaca
    41941 catgttttac agcgacattt ttatatagct taagaagatc gctgcattcg ctggcatctt
    42001 ccaataatgc atgattaaac gttccggcga aaatcgcagg cagattatca ataaatcgtt
    42061 gtgccgcgta gggtaccagt ttatttaggg tgtttacccg taaatacata aaaaactgat
    42121 cttccgtact gcggcttaaa ctatttgagc gtgatttttc ccaggcattt tcaaccacca
    42181 gcgaaaagag cgaacctttc tcatgctggc cccacgcctc gtgcaaatga tgataaagct
    42241 gctcaacggt aaatattctt ttctctaccg catcttcaag atctgccaca caataggaga
    42301 tgtcgtcggc agcttccata atccacgtta atggaaaacg actgtaaagc gccaaattaa
    42361 gttctttacg caaccgggca atataggctt cttcagaaag ataataacct ggctttttca
    42421 ttaaatagtg atgtgtctca ggcgtttcgc cacgccacca cgctggacgg gtatatttta
    42481 aaataccgcc aacctgcgcc caggtgagat tcatccgcat caatgtatgc accagacgaa
    42541 tgccttgtgc attcccctca aaatgacata agtcctgacg aatcttgcgc cgcagctcgt
    42601 taagcggttc ttccccgtcc cgtaaacgta gtgccgccac gctgcagcga tcgtcagtca
    42661 gaggctggct ttcggcatct tccgggtata aacgctggcg aaaccagtca tttatcgccg
    42721 cttcgccaaa atgaccaaac ggcggattgc cgatatcgtg catcaggcat gacatctcaa
    42781 caatgctttc aaaaggtccg gtcagttcat ccaggccgta tgcttccagc aatttaagct
    42841 ctttcagacg gcttaaaatt tctttggcga tgtagcgccc cacctgctgg acttccatcg
    42901 agtgggtaag acgcgtgcgc acggcggcat tgcgctccag tggaaaaacc tgggtctttt
    42961 gttgcagacg acgaattgcc ggagagttga tgatacgccc gcgatcgctc tcgaagatcc
    43021 gcaggatctc atgttcggtt ttaacgccct gcggtgaacg gtaacgacga tgccagttta
    43081 ttttttttcg gaaatcaatc tgtgccatgt cctctcccgc gtgagaaata cgcttccccg
    43141 taagcgcatg gtaaactatg ccttcaaatc gggcttatcg cgagtaaatc tatgaaaatc
    43201 ggcatcattg gtgcaatgga agaagaagtt acgctgctgc gtgacaaaat cgaaaaccgt
    43261 caaactatca gtctcggcgg ttgcgaaatc tataccggcc aactgaatgg aaccgaggtt
    43321 gcgcttctga aatcgggcat cggtaaagtc gctgcggcgc tgggtgccac tttgctgttg
    43381 gaacactgca agccagatgt gattattaac accggttctg ccggtggcct ggcaccaacg
    43441 ttgaaagtgg gcgatatcgt tgtctcggac gaagcacgtt atcacgacgc ggatgtcacg
    43501 gcatttggtt atgaatacgg tcagttacca ggctgtccgg caggctttaa agctgacgat
    43561 aaactgatcg ctgccgctga ggcctgcatt gccgaactga atcttaacgc tgtacgtggc
    43621 ctgattgtta gcggcgacgc tttcatcaac ggttctgttg gtctggcgaa aatccgccac
    43681 aacttcccac aggccattgc tgtagagatg gaagcgacgg caatcgccca tgtctgccac
    43741 aatttcaacg tcccgtttgt tgtcgtacgc gccatctccg acgtggccga tcaacagtct
    43801 catcttagct tcgatgagtt cctggctgtt gccgctaaac agtccagcct gatggttgag
    43861 tcactggtgc agaaactggc acatggctaa gtcactgttc agggcgctgg tcgccctgtc
    43921 ttttctcgcg ccactgtggc tcaacgcggc accgcgcgtc atcacgcttt ctcccgccaa
    43981 cactgaactt gcctttgccg ccgggatcac gccggttggg gtcagcagct attccgacta
    44041 tcctccacaa gcgcaaaaga ttgagcaggt ttccacctgg caggggatga atctggaacg
    44101 cattgtcgcg ctgaaacccg atctggtgat tgcctggcgt ggaggtaatg ccgagcggca
    44161 ggttgaccag ctggcttcgc tgggaataaa agtgatgtgg gtcgatgcga caagcattga
    44221 acaaattgcc aatgcgttac gtcaactggc cccctggagt ccgcaaccgg ataaggccga
    44281 acaagccgcg caatccctgc tggatcagta cgcgcaattg aaagcgcaat atgctgataa
    44341 acctaaaaaa cgtgtttttc tgcaattcgg cattaatccg ccatttacca gtggaaaaga
    44401 gtcgattcag aaccaggtac tcgaagtttg tggcggagaa aacatcttta aagacagccg
    44461 ggttccctgg ccgcaagtta gccgcgaaca ggtgttagca cgctcgccac aggcgattgt
    44521 cattacaggc ggaccggacc aaattcctaa aatcgaacaa tactggggtg aacagctcaa
    44581 aattcccgtt attcctctca cgagtgactg gtttgaacgt gcaagcccac gtattatcct
    44641 cgctgcacaa cagctctgta atgcgctttc acaggtagat tagcgcctga cctttttagg
    44701 tgctcagtgg ggattgaaca atgctcgtct attggctgga tatagtcggc acagcggtat
    44761 ttgccatctc cggcgttttg ttagccggaa aattgcgtat ggaccctttt ggtgttctgg
    44821 tactgggcgt ggttaccgct gtaggcggcg ggacaattcg cgacatggcg ctggatcacg
    44881 gcccggtatt ttgggtgaaa gatcccaccg atctggtcgt tgcaatggta accagcatgc
    44941 tgaccatcgt gctggtgcgc cagccaagac gcttaccaaa atggatgttg ccggtgctgg
    45001 acgccgttgg tctggcggtg tttgtcggca ttggcgtgaa taaagccttt aatgcggaag
    45061 ccggtccgtt aatcgcggtt tgtatgggcg tcattactgg cgttggcggc gggatcattc
    45121 gtgatgttct ggcccgcgaa atccccatga ttttacgtac agaaatctac gcaactgcct
    45181 gtattatcgg cggtattgtc cacgctacgg cttattacac attttccgta ccactggaaa
    45241 cagccagtat gatgggcatg gtcgtgacgc tattgattcg gctggcggct attcgttggc
    45301 atcttaagct accgacgttt gcgctggatg agaatgggcg ttgaagagag aagatgtgcc
    45361 ggatagttta tccggcacat gaacaacaga ttagatacta aaggaagaac cgcaaccgca
    45421 ggtgcttttc gcgttcgggt tggtcacgat gaaacgagaa ccttccagtc cttcggtata
    45481 gtcaacggaa ccgccgacca gatattgcag gctcatcgga tcaaccacca ggccaacgcc
    45541 ctgtttttcg atggtcatat cgccttcgtt cacctgatca tcaaaggtga aaccatactg
    45601 gaagccgctg caaccgccac cggtgatata cacgcgtaat ttcagattcg ggttatcttc
    45661 gtcagcgatc aggcttttaa ctttgttggc tgctgcgtcg gtaaactcca gcggcagtgc
    45721 tacgtcatca ctcatatttt gctccaaacg acatcggcaa ttgggcaaat tctaacccaa
    45781 taataatcgc cattatctaa taccctggta tttcgttcaa gtattctcgc tggctgatgc
    45841 ggccttgctt cgcgccagtt gctcagcttc ctgttttgcc agcgtgcgcg caagaatcgc
    45901 cgagtatagc ggtttcccgc cggtaaattg cgctaatagt gttgcgccaa gaccggtaat
    45961 aatcattggc aaaatgagct ggtagttatc ggtcatctcc agaaccagaa tgatccccgt
    46021 taacggcgcg cgaatagatg ccgccaataa cgcccccatt ccggcaatag caaacgtccc
    46081 cgcctcaagg tgatattgcg gaaacagctc aacggcaacc attccgaaag cggttcccag
    46141 cacagtaccc agcgccagca tcggggcaaa aataccgccc ggcgcgccgg aagagaagca
    46201 gagtaaggtg gtaatgaccc gcgcgacgaa gataaacacc agcattccca tgctgaaatt
    46261 ccccgcggta gcgataggaa tcaggttaaa accgccgccc gacgttgctg gtgcaacaaa
    46321 ccccagcaat ccacacagac cgccaatcgc accacccatc agtacccatt tggtaatatt
    46381 gccgccgtgc acacggtgca gcaaatcctg catccccagc acccatttat taaaaatagg
    46441 gccgaaaatg ccaaaaataa taccgaggat cagataaagc cacagcgtat taagcggcgc
    46501 gtcagaaagt ttaccgacgt caatcaacgc aacttcatga ttaaaaatcc ggtacataat
    46561 ggtcgacata atgacgccaa taaataccgc tttaatcgaa attaacgtat agcgaaactg
    46621 cggacgcatc tcttcgataa taaacaaaat acccgccagc ggcgcgttaa aggccgccgc
    46681 cagacccgcc gccgcaccgg ttgccaacag cgtatggcga gcttcatcac ctttcaggcg
    46741 gaaaacatcg agtaccatcc gcccgatatt accgccgatc tgcacggttg gcccttcgcg
    46801 ccctagcacc atgcctccgc cgagtgtccc cagcccgcca aagaatttca ccggcaatac
    46861 acgccaccag cgaacgggac gttgatcttc cagcgcccct tcaatttccg ggatccccga
    46921 accacctgct tccggcgcgt atttgcgcac taaaaagtag ccgaacatcg ccagcaccgc
    46981 cgaacagaga aaagcgacgg ttaacagaag cggataatta tcagcagtat gtaccagcgc
    47041 ccccatacgt tggttctgca accaggcgac acctttgtca aaagcaaccg cagccagccc
    47101 gacaagcgtg ccgacgactg ccgccataaa caaaatggct aacggggttt tatcgcgctc
    47161 aagaagttgg cgaatcagtt gtctgcgtcg caggcgcgcg gcctgcggtg tttctaaaga
    47221 gggagtatca gttttcatcc aatgatcact tattggtcat acaaataaga tgacggcatt
    47281 ttactcgcac gttccatgaa aatcctttgc aaagcgtaat gtttcaaatg cacaaaactt
    47341 ttatactcta agaaatcagg attgcaccag tacaagcagc ctgatgtttg acgagtattt
    47401 aacttgttat gaataacata gaatagcagc cattcacttt ttctacgaat caggaaccct
    47461 ccatgagtaa gtctgaaaat ctttacagcg cagcgcgcga gctgatccct ggcggtgtga
    47521 actcccctgt tcgcgccttt actggcgtgg gcggcactcc actgtttatc gaaaaagcgg
    47581 acggcgctta tctgtacgat gttgatggca aagcctatat cgattatgtc ggttcctggg
    47641 ggccgatggt gctgggccat aaccatccgg cgatccgcaa tgccgtgatt gaagccgccg
    47701 agcgtggttt aagctttggt gcaccgaccg aaatggaagt gaaaatggcg caactggtga
    47761 ccgaactggt cccgaccatg gatatggtgc gcatggtgaa ctccggcacc gaggcgacca
    47821 tgagcgccat ccgcctggcc cgtggtttta ccggtcgcga caaaattatt aaatttgaag
    47881 gttgttacca cggtcacgct gactgcctgc tggtaaaagc cggttctggc gcactcacgt
    47941 taggccagcc aaactcgccg ggcgttccgg cagatttcgc caaacatacc ttaacctgta
    48001 cttataacga tctggcttct gtacgcgccg cgtttgagca atacccgcaa gagattgcct
    48061 gtattatcgt cgagccggtg gcaggcaata tgaactgtgt tccgccgctg ccagagttcc
    48121 tgccaggtct gcgtgcgctg tgtgacgaat ttggcgcgtt gctgatcatc gatgaagtga
    48181 tgaccggttt ccgcgtagcg ctagctggcg cacaggatta ttacggcgta gtgccagatt
    48241 taacctgcct cggcaaaatc atcggcggtg gaatgccggt aggcgcattc ggtggtcgtc
    48301 gtgatgtaat ggatgcgctg gccccgacgg gtccggtcta tcaggcgggt acgctttccg
    48361 gtaacccgat tgcgatggca gccggtttcg cctgtctgaa tgaagtcgcg cagccgggcg
    48421 ttcacgaaac gctggatgag ctgacaacac gtctggcaga aggtctgctg gaagcggcag
    48481 aagaagccgg aattccgctg gtggtaaacc acgttggcgg catgttcggt attttcttta
    48541 ccgacgccgg gtccgtgacg tgctatcagg atgtgatggc ctgtgacgtc gagcgtttta
    48601 agcgtttctt ccatatgatg ctggatgaag gtgtttacct ggcaccgtca gcgtttgaag
    48661 cgggctttat gtctgtggcg cacagcatgg aagatatcaa taacaccatc gatgctgcac
    48721 gtcgggtgtt tgcgaagttg tgatgatttt gcctcgccca ccgggcgagg cattttatta
    48781 acggctctgc tttctcaaca aatagataaa atatggcgcg ccgataaagg tcgacagcag
    48841 ccccgccggg atctggaacg gaaacagcac catccgccca caccagtcag cgaacaccag
    48901 cagtaaacca cccaccagcg ccgaaatcac gatgtgcggc atcgtccgtc gaaagcccat
    48961 catccgcgcg atatgcggtg ccattaaacc aacaaagctc aacggtccaa tggtcatcgt
    49021 cgcggtcgcc gtcaggcaag ccgctaacag cagcagcgca attcgcgtcg gcgttaacgc
    49081 cacccccacg gcccgcgcgg tatcaccacc cagcggtaaa atggtcagcc agcggcggca
    49141 cagcggggta atcgccagca aaatcaccat cacaattccg gtgcgccaga cctgcgcatc
    49201 ggtggcgttg taggtcgaac cagagatcca ggtcagcact tgcgccattc gcgggtcacc
    49261 actcgcctgc aacatcatta aaagcatggt gaatgcggta cttaacgcca tccccgccag
    49321 taacatgcgg tgtggggaaa atccaccgcg cccggcggcg atcataataa tcaacagcgt
    49381 caccgcggca ccgatactgc ccgcgggtaa cagccagcca aaggcattac ccggcaccag
    49441 aaacagcatt aacaccacgc caaacgccgc gccagagcta atccccagca cttccgggct
    49501 tgccatcggg ttgccggtca gccgctgaat aatacagccc gccaccgcca gcatgacgcc
    49561 cgcaaacagc gccgccataa ttcgcggcca gcgccagggc attaaatcat cgagcaacgc
    49621 cccgctcgcc cacgtccagc cgtgcgcatc acgaccaaac gacagcgcca ccaccacagc
    49681 catcaacagc agcacgccgc ccgcgagggc aaacgccagc acatgttggc gttcagccgc
    49741 gacacgatcg ttgaccttca tatccggcgc gctaatgctg cgtaaacgcg gcagcaacca
    49801 cagtagcagc ggcgcaccga tcaacgcagt gaccgaaccg gtggacactt ccatccacac
    49861 gcgagtcagc cagaggatga tttgatcgga aagccagagg atcagcgcac caatcaacga
    49921 cgccagcatc agtcgcggca gcagacgccg cgcccccagc atttttgcca gcaacggcgc
    49981 gaacaacccg ataaagccga taatccccac cgcgttcacc agcagcgcac tgatgacaat
    50041 agccagcgac agcgccgcca gacgcgccag cgacaaggct agcccgagat tgcgcgccac
    50101 gccatcatca agccccatca gtgttaacgg acgaagtagc agcaacgtca gcatcacacc
    50161 gcccagcaac tgcggccata aacgctcaac accgccccag tcggtttgcg tcagcgttcc
    50221 agtgctccac agaaacatgc tttgcagttg gtcatgatgg aagataacca gtaactgatt
    50281 gattgcgccg caataaaggc tcactaccaa ccccgcgaga atcagcgtta ccggcgacag
    50341 ccgtttcccc cacgcgacgc caaagacaat taagccaaca acacaagccc ctgccagcgc
    50401 agcaaactgg ctcgccatcg caccagggat cgcccagagc gtcgtgacgg taatccccag
    50461 ttgcgcgcct gtagcaacgc ccaacgtcgt cggttccgcc agcgggttac gcagcacttg
    50521 ctgaaacagc acgcccacca gccccaggcc cgcgcccacc agtagagaaa tcgccagacg
    50581 cggtaacaag ctgtagtgaa aaatcatctg ctcgatgacg ttaatatccg gcgaccaggc
    50641 ggcctgcgcc cactggctac gcggcagcgc ctgcaaaaag ttcatccagg tcagcgccgt
    50701 agcgacaatc accagcagcg ccagcaataa cgccgggaaa agcgcaatac gtttactcac
    50761 gctttacctc caatggcgtt atccagaacg cgcacaaagt gcattgccga gagcgttgca
    50821 ccataaaacc agactgcggg tacgcgctga aagcgtccgg cgcggacaaa cggcatggcc
    50881 tgccacagcg gcgttgccat tagcgcatcc atgtctttgc tgttgtcgtg atcaaaacag
    50941 agcacatcaa cgtctttata cgctgccaga cgatcgatgc tgacggcggt actgccccag
    51001 aagttggttt ccccttgcca ggcatttggg atgccgtact catcaagaat ttcctggaac
    51061 aagctgtttg gaccgaagac cagcatatgg cgcggatcga taagcgtcgt cagcaataac
    51121 ggacgcgcac cacgcttcac aaagcggggt ttcatgctgc ggataaagtc ttcatattgc
    51181 gctaaatgcg tttctgctgc gctttgcagg ttaagtaaat ccgccatttc cgtcagcgat
    51241 ttacgcgcca tcgccagcgg ctgtttgccg tcgctgaagt taaatccacg ccccggcgca
    51301 atacgagcca gcatttctgg cgaaggacca tatcccgccg accagaccat aaacgacggt
    51361 ttcatttcgg tcagcagttc gaggttaggt tctgtacgca aaccgacgtc aatcaccgat
    51421 tcaggtagtg gtggttcgct gacccacagg cgatagttga tggtgtccgc cacgccgtaa
    51481 ggcacgatgc cgagcgccag tagtaattcc accggcagcc actccagcgc cacaatacga
    51541 tttgggtcaa tagccgccgc gtgggcggta ttcatctgcc ataacaacgg agaaagcgcc
    51601 atcgccgtta acagtcgacg gcgcgaaata agaggtaagc cgctcatcaa taaacaaaac
    51661 tcacaggtgc agcacccgcc ggatgcggca gaatacccat cgggatgcca taaatcattt
    51721 cgagggtttc gccgcgcata atttccgcag gcgttccctg agcaatcatt tcaccgccgc
    51781 gcagggcgac cagataatca cagtaacgcg cagccatatt gatatcgtgc agcacggcaa
    51841 tgaccgtcag gccacgctcc tgacttaaac ggtgcaccag cgccagcaca tcaacctggt
    51901 gggcgatatc caacgccgaa gtcggttcgt cgagcaacag acaacggcta tcctgcgcca
    51961 ccagcatggc gatccacgcc cgctgacgtt cgccgccaga gaggctttct accagccgat
    52021 gcgccagcgg ttttaagcca accagcgaga tagcttcctc gaccttttcg cgatctgccg
    52081 caccaaagcg ccccagcgcg ccatgccacg ggtaacgccc aatcgccacc agttcacgca
    52141 cagtcatccc ttctgccgga ggaagttgct gcggcaaata agccactttg cgggcaaacg
    52201 ctttgctact ccagctttcc agcggctggg cttcaagaag aatttcacct tctgacggcg
    52261 gctgatgacg gcccaacatt tttaacagcg tggatttacc agaaccgttg tgaccaatca
    52321 aaccggtcac tttcccggca ggaaaggtta acgacagcgg atgcaaaagc gtgcgccctg
    52381 gcacacgaaa ggagatatta cgcagtgcaa aagtggtatc ggaatgattc gtgtattcct
    52441 gcataacagc caacttgtga agcgggcacg gaaacccgtg ccccaaagag aaaattagaa
    52501 acggaaggtt gcggttgcaa cgacctgacg ttctgcgccc cagaagcagc cataggtctg
    52561 gaagcaactg gcaacatact cacgatcgag caaattgttg acgttcacag caaggctgga
    52621 tcccgccata ccaaagcgtg ccagatcata ttttacaaca gcatccatta cagctgcgct
    52681 acccacttta aaagtgtttg ccggatcacc atagctggaa ccaataaaac gaccacctgt
    52741 tcccaatgtt aaaccagaaa gcggcccttc attgaaggta taatcccccc agagagatgc
    52801 catatgctcc ggtacttgtt caggagtttt acctttgaga ttggtgtctt tggtgtattc
    52861 cgcatccgtg taggtataag aagccgtcaa gttaatattg gcatttaacg ccgcttttgc
    52921 ttcaagttcc acaccacgag cgcgaatctc acctgctggg acttgcgcta acgggtttgt
    52981 tgggtctgct gtcaggttat ttgttttcgt caattgatag actgcgcccg taacaacgac
    53041 cggcatatca ttcggaacat attttacgcc agcttcatac tgttcacctt tcgatggctt
    53101 ataggaaacg cgcgggttgc tccacagatc gaaagcactc ggttcaaagg actggctata
    53161 gctaaagtaa ggtgaaatac cattatcgaa taagtagttt acaccaccgc gccaggtgaa
    53221 ctgatgatca ttacgctcaa tataaccttc agtcggcgta taagagtttt cacgaacagt
    53281 ggtcgcttgc ttagaccaat cgtagcgtcc ccccagagtg aatacccatt tattccattc
    53341 cgcctgatcc tgaacataaa tacctgtttg tttgctttca ttcatttggt atggctctgc
    53401 gttaccaaat gcaaagtatt caggatgata tttgttataa agatcgatgg atggtgcaga
    53461 tccaaagctg gcattgatat cattgcgcat acgcatgaag tctacccctg tcagcaaagt
    53521 atgctcgact tcacctgtag caaatttact ttcaagttgg gtatcaacgc taaagttttg
    53581 cagacgctca ttatccacca ctgtcccgcg gtttagagta tgaccatcgt tggcaatccc
    53641 tgtgccataa acacttttct gtgaggtttt catttcactg aaacgcagat tctgacgcac
    53701 ggtgaaggtg tcattaaaac catgttcaaa actatatccc accattttct ggttacggga
    53761 gtatgtatta ttcgatgcac cttcattgaa gtcagtcggt agacgtttac cattaggcaa
    53821 tggctcaacg gtcccctctt tcggcaacca accgtaataa cccgtttctg gttcattctg
    53881 gaaataggac aggaaagtaa aattggtttt gtcgtccgga cgccatgaga atgatggagc
    53941 aatggtataa cgctgagatt cagaattttt ctgctgttcg tttgttgaac gagcgagccc
    54001 cgtcaaacgg taggagaact caccgttatc atccagcgca tcactgaaat cgaatccggt
    54061 ctgaaacaga ttatccgtcc ccattttaaa ctgaatttct ttcaggggtt caatggtcgg
    54121 acgcttactc accatagaaa taatcccacc aggattactt tttccataga gaacagacgt
    54181 cggcccacgc atcagttcaa ccctctcgag catgtaagga tcaatcacag catcgttata
    54241 aaagttgccc tgaagtttta gtccatcaag gtagttattt tgattcagac caacagatga
    54301 gaaaccacga atgataacaa aatcatatgt attagaagca ccacgactgc taaccgtaac
    54361 accaggtgta taacctaatg cttcttttac agactgaaat tgatgcatct gcatctcttc
    54421 atttgtcaca accgaaatag actgcggcgt tttttcaatt ggtgtatccg ttttagtcgc
    54481 agtagcagag tgcttcgccg caatagttgc agcaggcccc catgcgcttt cttgcggcgc
    54541 aggtgcagca gtaacggtga tagtgtcttc tttcggttca accgctgcct gtgcataaac
    54601 agacatgccg ctaaccgctg tggctactac aactgcgatt ttacgcagtg agtgttttgg
    54661 ctgagcagtt ttggaacgcg ccattggtat atctctgatg taaagtgaat gataacgtaa
    54721 acgagaatga ttattataaa gataagataa tattcgaaac gctggcgggt tcgcaagcgg
    54781 tacgcaatct gagcgttcct gaagggttaa taaacaacca gatgaaaaga aagggttaat
    54841 actttaggtg ggatgttatt ttaccggatg gcaactcgcc atccggtatg tcacgtttag
    54901 acgttaatta ctaccaaaca tatctttaat ccaaccggct acaccgtcgc tgtctttctg
    54961 ctcttgctga gcaggttgct gttgcggctg ttgctgcggc tgagaagact gatcaaacgg
    55021 attgcctgac ggctgctgct gcatctcact ctgctggcac agcgattgcg gatcgctggt
    55081 ccagaccggc aagacacgca tgccaccgct gcaaacaaag ttgccgtcgt agtccacgcc
    55141 catatctgca atatcttctg gcggaacaag attcagcggc gttggcgtct ggttagccag
    55201 ataacgctga taaatcgaca ttgccccgct ggcaccatac agtttggtcg gctggttgtt
    55261 atcacggccg acccaggtga tggtcaccgt gctgccgtca atgcccgcaa accatgtatc
    55321 tacgttattg ttggtagtcc ctgttttccc tgccagatgc aggttcgggt atttcgcccc
    55381 aagctgacga cccgtaccgc gttgtaccac ctgctgcatg gtccatagtg tcagatacgc
    55441 cgcctgcgcc ggaacagcgc gttccgcctg cgggaagctc tgatacagca ctttgccatc
    55501 ttccgcaatt accgaacgca gcgcagaaag cggtgcacgg ttaccgccgc tggcgatggt
    55561 ctggaatgcc tgcgccactt cgattggcgt taagttcaac gcccccagca gcatcgccgg
    55621 aaccggatgc agctgatctt tcggcacgcc cagtttaatc caggtatccg taaccgcagg
    55681 cagccccagc gccatcccca gatttaccgt cggcacgttc atcgaacggg tcaacgcatc
    55741 caccagcatc actctgccgc tttcgctata acgacggtca tcgttctgcg gtgaccagac
    55801 ctggccattc ggctgacgta gcgcgattgg cgcatccgca atccacgtat tcagacgata
    55861 gattttcggc tggcttaaag ccgtcagata agtcgctggt tttgcaaggg aaccaatcga
    55921 acgccgcgcc tgcatcgcac ggttgtagcc cgcaaactgc ggctcagaac ctccgaccat
    55981 cgcacgaact tcaccactaa agcggtcgac gaccacaatc gcggtttcca gatcgctcag
    56041 cttacgctgt ttcttaagtg ccggaatgcc ttccacggcg gctttttctg ccgcgtcctg
    56101 ggccaccgag tcaaaggtag tgaagatctt cacgccggag agatctttta ccttatcgcc
    56161 taatttagcc tgaagctcct gacgcaccag ttgcataaag gctggctgag gagagatcac
    56221 cccaccgcgc ggctgaacac ccagcggacg cgcactcaac atgtcataga gttcttgatc
    56281 aataatctgt tgctgttgca gcagacgcag taccagatta cgtcgctcca gcgccagttt
    56341 cgggttacgc cacgggttgt agatggacgc tcctttcacc ataccgacta acagcgcctg
    56401 ttgatcgagg ctcaattctt ctaccgggcg accaaagtaa tacaagctcg ccagcgggaa
    56461 gccgcggatt tcgttgtcgc cactctgacc gagatacacc tcgttcatat acagctcaag
    56521 aatacggtct ttgctgtaac gcgcgtccat gatcagcgcc atgtaagctt cgttcgcttt
    56581 acgccagtaa gagcgctcgc tggagaggaa caggtttttc accagctgtt gcgtcagcgt
    56641 actcgccccc tgcaccgtgc gtccggctgt caggtttgcc agcaccgcac gtccgatgga
    56701 gtagagactg attccatcat gctcgtagaa atggcggtct tctgtcgcca gcaaagtatc
    56761 caccagcaaa tccgggaaac cactgcgcgg cacaaacaaa cgctgctcac cgtttggtga
    56821 ggagatcatg gtgatcagac gcggatcaag acggaagaaa ccgaactgac ggttgttctc
    56881 catattgacg atcgtcgcca aatgatcgcc atcaaaggtc agacgcgcgc gcacctgtcc
    56941 ttctttactg tccgggaaat caaacggacg gcgaatcatc tcaatgctgt tggcctgcac
    57001 ggtaaattcg ccaggacggg tcatttttga cacctgacga tactgggttg cctccagcag
    57061 cttcaccatt tcgttcttgc tgatggtcat gtctggctca aggttgacca ttcgaccata
    57121 aactgccgca ggcagttgcc agaccttgcc atcaatacgg ctacgaattt tttgatcgag
    57181 ataaacgccg taaatggcga tcagcacggc aaaaacgata gccagtttta acagcagcca
    57241 tagccagccg cgtttgccac gaggcttacg ccctttgcct ttgcccttac ctttgcgcgg
    57301 catcggttct tcatcctcat agtcatcata atcgtcgtaa tcatcgtcat cttcgtaacg
    57361 acgacggctt accttttgtt tgaccggacg cgtcggtttc cctttgcgtc caattggctc
    57421 gcggtcattc ccggccatgc tttttctccg caatattcag gcgcaaaagc ccgattttct
    57481 gttcttctgt tacaagacag aagaagatat ctctcaaaat ttgtgctcga tcggtcccct
    57541 ctcccttaag ggagagggtt agggtgaggg tgaacgtgcg cgctgatgcc ctcaccccgg
    57601 ccctctccca cagggagagg gagaaaacca accttacgaa tactttttcg tccgtcgcgt
    57661 cggtgccgtg tttgccgggt cgtccggcca gacgtgtttg ggataacgcc ctttcatctc
    57721 cttttgcacc tcacgatacg cccctttcca gaaggccccc aaatcacgcg tgatttgcag
    57781 cggcctttgg gcaggggaaa gcaactccag caccagcggc acgcgcccct gggcgatcgt
    57841 cggattggtg gcctcaccaa acatctcctg cattctcacc gccagcgcgg gcgggttatc
    57901 ttcatgataa cgaatggcga tccggcttcc cgtcggcaca gtgtaatgcg caggcaattc
    57961 actatccaga cgttgctgca ttccccaatc aagtaatccg cgtagtgcct gataaatgtc
    58021 gagtgatttc aggccgcgta gtgaatgtac accggccata tgtggcagca gccacgtttc
    58081 cagcgtcgcc aacaaacttt catcatcaac cgctggccag tcatattctg gcaaccactt
    58141 tccggcgcat aacaaacgca agcgtagctg ttccgcttcc gccgtccagt tgagcacgct
    58201 taaaccttta tcacggatgc cgttaagcat cgcctgatgc aactcgtctt ctgacggttt
    58261 cgccaacggc tgcactttca ccgtcaactg accgatttgc agccgacgcc aggctttcag
    58321 cgtaccttgc gcgtcatccc attccacggt gtcagattgc tgcaccagct gcggacagcg
    58381 tcgcactaat tcatcaatat cgaccggtag cgccagtaaa atccgcgcat ccggcgaggc
    58441 gctgccctgc aataataacg gtgcgatcaa ccattcgtgg cggcttagcg cgtcgtcggc
    58501 atcgagcatc gctcccatgc cgtttgccag ttgatagcgt ccatcttgcc cacggcggcg
    58561 agcaatgcga tcggcaaacg ccccggcaag tagcggcgcg ataagcgaac tgtctgcctc
    58621 accgccgcgt acgtttaagc gttttaacag ttgctgacta cgttgctgcc aggctggttg
    58681 attgcgcgaa aacgccacgc ccaggtcact attgcccatc cgtggcggct cttcgagaat
    58741 ggcggcaatt tttgccgcgg tagcagcttc gtcgtcgctc ttcgcgctca ccagcattgc
    58801 cgctaaacgc ggatcgttac ccagcgctgc cattttttgc ccttgcgcac taagccgttc
    58861 accgtccagc gcccccagca tttgtaacag acgtttcgcg gccagtagat tcaccgttgg
    58921 cggttgatcc agccagctca tctgcgccgg atcgctgcat ccccattgca gtaattccat
    58981 cagcaaaccg gaaagatcgc tttgtaagat ctccggttca ctttgcgccg cggcgcgttc
    59041 tgcttgttcc ttggcgatta aatgcaggca gatacccggc tccagacgcc cggcgcgccc
    59101 ggcacgctgc gtcatggatg cctggctaac gcgttgagta atcagtcgcg taagccccgt
    59161 gcgcggatca aaacgcgcca cacgctcctg ggcacaatcc accaccagac taataccttc
    59221 aatggttaaa ctggtttcag caatattggt cgccagcacc actttgcgca tcccttgcgg
    59281 tgccgggagg atcgcttttc gctgatcgtt cagcgacaac gcgccataca gcgggcagag
    59341 caacacatca ctgccgatgc gcgaagccag ttgttcctgc acacgctgaa tttctccgac
    59401 gccaggtaaa aataacagta atgatccact ttcctgacgc agcatttcgg cggtggcaac
    59461 cgcgacggct tcgtcaaaac gctgatgcgt gggcagcggt aaataacggc gttcaacagg
    59521 aaacgagcgc ccttctgaga tgacgacggg cgcttctggc agcatttgct gcaagcggtc
    59581 gttatccagc gtagccgaca taatcagcag tttaaggtca tcacgcagac cttgttgcac
    59641 atcgagtaac agcgccaacg ccaaatccgc ctgcaagctg cgctcatgaa attcatcaag
    59701 gatcaccagt ccaacaccgc tcagttccgg gtcacgctgg atcatccgcg tcagcacgcc
    59761 ttcggtaact acttccatgc gggtattcgg cccgacgcag ttttgcgcac gcatccggta
    59821 gccaacggta tcgcctggct tttcgttaag cagttccgcc agccgttgcg cgacgttacg
    59881 cgccgccaga cgacgcggct ccagcaggat aattttcccg ttaatgccgg gatgcgccag
    59941 cagttgcagc ggcagccagg ttgatttccc ggccccggtc ggcgcactta ataatacctg
    60001 cggcgcacaa tcgagagcgg taagtaattc aggtaagacg gcagcaacgg gcaacgacga
    60061 cacaaaacgc tccagagggt taacattctt cgcgccacat tgtagcatcg cggtaattca
    60121 taaccgagtg cctcacatgt ctgacccgca acgtctgttc tttgctatcg acttacctgc
    60181 agaaatccgc gaacagatta tccactggcg cgccacacac ttcccacttg aggcgggacg
    60241 tccggtcgcc gccgataatt tgcatctgac tctggcattt ttaggcgaag tgagcgccga
    60301 gaaagagaag gcgctttctc ttttagccgg acggattcgt caacctggtt tcacactcac
    60361 gcttgatgac gccggacaat ggctgcgttc gcgtgtggtg tggttaggga tgcgtcagcc
    60421 tccacgcggc ttaatccagc tggcgaatat gctccgttca caggctgccc gcagcggttg
    60481 tattcaaagc aatcgtccgt ttcatccaca tattacctta ttgcgcgacg ccagcgaggc
    60541 ggtgacaatc ccgccgccag gttttaactg gtcgtatgcg gtgacggagt tcacccttta
    60601 cgcctcctcg tttgcccgtg gacgcacacg ctacacgccg ctaaaacgct gggcgctaac
    60661 gcaataacaa ggattgtcgc aatggaattt tctccccctc tacagcgcgc gacgctaatt
    60721 cagcgttaca aacgtttttt agccgatgtg atcacacccg atggtcgcga attaacgcta
    60781 cactgcccga atacgggtgc gatgaccggt tgtgcaacgc ctggcgatac cgtctggtat
    60841 tcgacttcag acaacactaa acggaaatac ccacacacct gggaattaac tcaaagccag
    60901 agcggcgcat ttatttgcgt caacacgctt tgggctaaca ggttgacgaa agaggctatc
    60961 cttaatgaat caatttcaga actgtcaggc tatagctcgc tgaaaagcga agtaaaatac
    61021 ggcgcagaac gcagccgtat tgactttatg ttgcaggcgg attcgcgtcc agactgctat
    61081 attgaagtga aatcggttac gttagcggag aacgaacagg gatattttcc cgatgcggtc
    61141 actgaacgag gtcagaaaca ccttcgggag ttgatgagcg tagcggctga aggccagcgt
    61201 gcggttatct ttttcgccgt gctgcattca gccattacac ggttttcacc cgcgcgccac
    61261 atcgatgaga aatacgcgca actattgtca gaagctcaac agaggggggt agaaattctg
    61321 gcttacaaag cggaaatttc tgctgaaggc atggctctta aaaaatcact gccggttaca
    61381 ttgtagtaaa gtaagtaact ggttaattta cattctggtc gcgtgcgcaa atacgctttt
    61441 cctcacacag ttgtcaagtg ttacgtttag ataattgcta tccggaaaag catctgctat
    61501 ttatagcggc ctcatttttc ccccgaacat ggggatcgat agtgcgtgtt aaggagaagc
    61561 aacatgcaag aagggcaaaa ccgtaaaaca tcgtccctga gtattctcgc catcgctggg
    61621 gtggaaccat atcaggagaa gccgggcgaa gagtatatga atgaagccca gctggcgcac
    61681 ttccgtcgta ttctggaagc atggcgtaat caactcaggg atgaagtcga tcgcaccgtt
    61741 acacatatgc aggatgaagc agccaacttc ccggacccgg tagaccgtgc agcccaggaa
    61801 gaagagttca gcctcgaact gcgtaaccgc gatcgcgagc gtaagctgat caaaaagatc
    61861 gagaagacgc tgaaaaaagt ggaagacgaa gatttcggct actgtgaatc ctgcggtgtt
    61921 gaaattggta ttcgccgtct ggaagcgcgc ccgacagccg atctgtgcat cgactgcaaa
    61981 acgctggctg aaattcgcga aaaacagatg gctggctaat tacagccgtt ccatcacgtt
    62041 taccacaggc gggaaatgct cccgccttat tttttgttca aagagatgac agacacacac
    62101 tatattggcc gcttcgcccc ctctccttcc ggcgagcttc attttggttc tctgattgct
    62161 gcgcttggca gctatttgca ggctcgcgcc cggcaaggtc gctggctggt acgcatagaa
    62221 gatatcgacc cgcctcgtga agttcccggt gccgcagaaa ctatcctgcg ccagctggaa
    62281 cattacggcc tgcactggga cggcgatgtt ctctggcaat cgcaacgtca ccacgcctat
    62341 cgcgaagcac tcgcctggtt acatgagcaa ggactaagtt attactgcac ctgcacgcgt
    62401 gcacgtattc aaagcattgg cggtatttac gacggtcatt gccgggaatt gcatcatgga
    62461 ccaggcaacg ccgcagtgcg tatccgccag cagcatccgg tcacccagtt tactgacctg
    62521 ctgcgaggca ttattcacgc cgacgaaaaa ctggcacggg aagattttat tattcatcgc
    62581 cgtgatggtt tgtttgccta taacctggcg gtggtggtgg atgatcactt ccagggcgtg
    62641 agcgaaattg tccgcggggc ggatttgatc gaaccgacag tgcggcaaat ctcgctgtac
    62701 cagctttttg gctggaaagt gccagattac attcatctgc cgctggcgct taatccacaa
    62761 ggcgctaaac tttccaagca gaatcatgcg cctgcgttgc cgaaaggcga tccacgcccg
    62821 gtactaatcg cggcacttca atttctgggt cagcaggtag aagcacactg gcaggatttc
    62881 agcgtcgaac aaatccttca gtcagccgtc aaaaactggc ggcttaccgc agtgcctgag
    62941 tcggcaattg taaattcaac attctcaaat gcgtcatgct gagctatgat tagccgctat
    63001 ttttttgtcc tgaatgatgt ttgacactac cgaggtgtac tatttttacc cgagtcgcta
    63061 atttttgccg caaggtgcta agccgcgagg aaagcgaggc tgaacaggca gtcgcccgtc
    63121 cacaggtgac ggtgatcccg cgtgagcagc atgctatttc ccgcaaagat atcagtgaaa
    63181 atgccctgaa ggtaatgtac aggctcaata aagcgggata cgaagcctgg ctggttggcg
    63241 gcggcgtgcg cgacctgtta cttggcaaaa agccgaaaga ctttgacgta accactaacg
    63301 ccacgcctga gcaggtgcgc aaactgttcc gtaactgccg cctggtgggt cgccgtttcc
    63361 gtctggctca tgtgatgttt ggcccggaga ttatcgaagt tgcgaccttc cgtggacacc
    63421 acgaaggtaa cgtcagcgac cgcacgacct cccaacgcgg gcaaaacggc atgttgctgc
    63481 gcgacaacat tttcggctcc atcgaagaag acgcccagcg ccgcgatttc actatcaaca
    63541 gcctgtatta cagcgtagcg gattttaccg tccgtgatta cgttggcggt atgaaggatc
    63601 tgaaggacgg tgttatccgt ctgattggta acccggaaac gcgctaccgt gaagatccgg
    63661 tacgtatgct gcgcgcagta cgttttgccg ccaaattggg tatgcgcatc agcccggaaa
    63721 ccgcagaacc gatccctcgc ctcgctaccc tgctgaacga tatcccaccg gcacgcctgt
    63781 ttgaagaatc gcttaaactg ctacaagcgg gctacggtta cgaaacctat aagctgctgt
    63841 gtgaatatca tctgttccag ccgctgttcc cgaccattac ccgctacttc acggaaaatg
    63901 gcgacagccc aatggagcgg atcattgagc aggtactgaa gaacaccgat acgcgtattc
    63961 ataacgatat gcgcgttaac ccggcgtttt tgtttgccgc catgttctgg tacccactgc
    64021 tggagacggc acagaagatc gcccaggaaa gcggcctgac ctatcacgac gctttcgcgc
    64081 tggcgatgaa cgacgtgctg gacgaagcct gccgttcact ggcaatcccg aaacgtctga
    64141 cgacgttaac ccgcgatatc tggcagttgc agttgcgtat gtcccgacgt cagggtaaac
    64201 gtgcatggaa actgctggag catcctaagt tccgtgcggc ttatgacctg ttggccttgc
    64261 gagctgaagt tgaacgtaac gctgaactgc agcgtctggt gaaatggtgg ggtgagttcc
    64321 aggtttccgc gccaccagac caaaaaggga tgctcaacga gctggatgaa gaaccgtcac
    64381 cgcgtcgtcg tactcgtcgt ccacgcaaac gcgcaccacg tcgtgagggt accgcatgac
    64441 agtggcgtat attgccatag gcagcaatct ggcctctccg ctggagcagg tcaatgctgc
    64501 cctgaaagca ttaggcgata tccctgaaag ccacattctt gccgtttctt cgttttaccg
    64561 cactccaccg ctggggccgc aagatcaacc cgattactta aatgcagccg tagcgctgga
    64621 aacctctctt gcacctgaag agctactcaa tcacacacag cgtattgaat cgcagcaagg
    64681 tcgcgtccgt aaagctgaac gctggggacc gcgcacgctg gatctcgaca tcatgctgtt
    64741 tggtaatgaa gtaataaata ccgaacgcct gaccgttccg cactacgata tgaaaaatcg
    64801 tggatttatg ctgtggccgc tgtttgaaat cgcgccggag ttggtgtttc ctgatgggga
    64861 aacattacgt gaaatactca cttcactagc agtaccacaa cctgccatct ggtaatagct
    64921 gtattatttt attttcttga cgagaggaat acaaaatgat acggctattt catccaccaa
    64981 gaaaaattat tttcttatct tactcctcac atcccccctt acacattaaa tatacttaat
    65041 ttacatttta gtttttttta attcattcat gaaatagcac ttcatgcaaa tagattaggc
    65101 attttttgtt taaacgacct aaaataacaa caattcaata cgccacataa atatattcac
    65161 acccgtgata actcacccct attacatcct ccaaaaactt tgagtaatat ccgctcgcaa
    65221 tattaatttc atataatttt tgtaaggatg catgtaatga aaaaagcact tctcgcagcc
    65281 gctctggtta tggcttctgg ttccgccctg gctgtagatg gtggtcatat cgactttaac
    65341 ggtatggtac agtccggtac ctgtaaagtg ggtgtggtag atactggtat gcacagcgtt
    65401 accactgatg gcgtggttac cctggatact gcgaatgtta ctgatacttt tgctgaagtt
    65461 agcgcaactg ctgtcggttt actgccgaaa gagttcatga tttctgttga gtgtgatcca
    65521 ggtgctccga agaatgctga gttaactatg ggttctgcaa gctacgcgaa caccagcggt
    65581 accctgaata acaatatgaa catcactgtt aacggtattg aaccggctca gaacgtaaac
    65641 attgcagttc ataacatgaa aaacaaagct ggcgctgctg aaattaagca ggtccatatg
    65701 aacaactctt ctgaagttca ggaactgaca ttagacgcag aaggtaaagg ccagtacgta
    65761 tttaacgcat cttacgttaa agcaccgaac agcccggctg taactgctgg tcatgtaacc
    65821 actaacgcgc tgtacaccgt tgcttataag taattgatgg tgtgttgagc ataaggtttg
    65881 ccagcaggca aaccttattt tcgcgaattt aatctttctg cagacagcaa ggagtgctta
    65941 tgtttccctt ggttaaaaaa acggttagtg cattatttgt gagtactctg ttagccagtg
    66001 caccagcatt cgctgacatt attatttccg gaacacgtat aatttataac gctgataaaa
    66061 aagatgttaa cgttcgtctg gaaaataaag ggaatcgccc cttactgatt cagaactggt
    66121 tggatactgg cgacgacaat gccgatccca gccagatcaa agtgccgttt accgcgaccc
    66181 caccagtttc acgtgtggag ccaaaacgcg gtcaaaccgt aaaagtgatg tataccggtg
    66241 caacagccct gccgaaagac cgtgaaagcg tctactggtt taacgtgctg gaagtgccgc
    66301 ctaagccgaa agatgctgag gctgacaaaa acttgttaca gctggctttc cgcacacgca
    66361 tcaaactctt ttatcgcccg agtgggttac agggagaacc ggctgaagca ccggcgaaaa
    66421 tcacctggaa attaaacaac gctcagttac aggcaaataa cccaacgcca tattacgtgt
    66481 cctttaatga ggtcaagctg gaaagcggcg gcaaaaccta caacgtcaat tccagcatgg
    66541 tcgcgccgtt cagccaggca agctttggcg taaccggtct gccaggaagc gtgtcatccg
    66601 gaaaagtggt ttttaaagcg atcaacgact ttggcggcaa cattgatggt tccgcaacat
    66661 tctaaaaaat aaacggattc tttttaacat cgccaggaat gcaaccttgt accagtttac
    66721 tcatcaaaaa agccgtatcc cgaaaaaaac gctacttgcg gcctgttgcg ccctttttta
    66781 cagcggcaac agttctgcgg cagacacggt ggaatatgac agttccttct taatgggaac
    66841 tggcgcatca acgattgatg ttaaacgtta tgctcaaggc aacccgacac cgccgggtct
    66901 ctataatgtc cgcgtgtttg taaacggtca ggcgacgtcc agcttagata ttccgtttgt
    66961 ggatattggc gaaaacagcg cagcggcctg tattacccac aaaaacctgg cacaacttca
    67021 cattaagcaa cctgaacagc ctgtcacttt actcgccaga gaaggtgagg aagaggattg
    67081 tctggatttg gcaaagtcat acgaaaaggc ggatgtgcgc tttgacggta gtgaccagtt
    67141 tctcgatctg acgatccctc aggcctatgt tttgaaaagc tatggcggct acgttgaccc
    67201 ttctttatgg gaatcgggaa ttaacgctgc cacactggca tataccctga acgcgtatca
    67261 cacaagttca gataacgaca atagtgacag cgtctatggc gcatttaact cgggtatcaa
    67321 tttaggagcc tggcacttcc gtgcgcgcgg taactataac tggacaacag ataacggcag
    67381 cgatttcgat ttccaggatc gttacttaca gcgtgacatt ccggcaatcc gttcacagat
    67441 aattatgggt gacgcctaca ccaccggtga aacgtttgac tctgtcaacg tccgtggtgt
    67501 tcgcctgtac agcgatagcc gtatgctacc ttcggcgctg gccagttacg ccccgaccat
    67561 ccgcggtgta gcaaactcca acgccaaagt tacggtgacg cagaacggtt ataaaatcta
    67621 tgaaactacc gttccgcccg gtgaatttgt tatagacgac attagcccct ccggctttgg
    67681 tagcgaactg gtcgtaacca ttgaagaagc ggatggttcc aaacgcacct ttacgcaacc
    67741 cttctcgtcg gttgtgcaaa tgcaacgtcc tggtgtaggt cgttgggatt tcagtgccgg
    67801 taaagtcatt gatgatagcc tgcgttccga acccaatatg gggcaagcct cttattacta
    67861 tggtctgaat aacctcttca cgggttatac cggcattcag ttcaccgata acaactatct
    67921 tgccgggctg ttaggtgtgg gtatcaacac cagcatcggc gcctttgcgg tagacgttac
    67981 ccattcccgt gctgaaattc cggatgataa aacctaccag gggtaaagtt atcgcgtgac
    68041 ctggaacaaa cttttccagg ataccgggac atcatttaac ctcgcggcgt accgctattc
    68101 cacccaggat tacctgggcc tgcatgatgc gttagtcctc attgacgacg ccaaacattt
    68161 gtctgccgat gaagacaaaa acaccatgca gacgtactca cgtatgaaaa accagtttac
    68221 cgtcagcatc aaccagccgc tgaaaacgac ttacgaagat tacggttcac tgtttatttc
    68281 cggtagctgg acggattact gggcggcgaa caatagccgc actgaatata atgttggtta
    68341 cagtaaaagc gtttcgtggg gcagtttcag cgtcaacctg caacgtagct ggaatgaaga
    68401 cggcgagaaa gatgacgcga tgtacgtcag cgttagcgta cctattgaga atattttagg
    68461 tggcaagcgc aaatcttctg gtttccgcaa tttaaatact cagctcaata ccgatttcga
    68521 tggttcacat cagttgaatg ttaacagttc cggtaacact gaaaacaatc tggtgaacta
    68581 cagtgtcaac gctggttata gcctcgataa aaactccggc gatttagcct ctgttggcgg
    68641 ttatctcaac tatgagtctg ggttaggcgg tatttccgct tcggcctcgg ccacttctga
    68701 taatagccag cagtactcca tctccaccga tggcggcttt gtattacaca gtggtggttt
    68761 aacgttcact aacaacagtt tcagcagtaa cgatacgctg gtgttaatca acgccccagg
    68821 tgctaaaggc gcacgaatca ataacagtaa taacgaaatc gatcgctggg gatatgccgt
    68881 gacgtcctct gtcagcccct accgtgaaaa ccgggtaggt ctgaacattg aaacactgga
    68941 aaacgatgtt gaactgaaaa gtaccagcgc aaccaccgta ccacgcagcg gctccgttgt
    69001 tttgacccgt ttcgaaactg acgaggggcg ttctgccgtg ctgaatatta ctgccgccaa
    69061 tggcaaatcc attccgtttg ctgcggaggt ttaccagggt gaggtgatga tcggcagcat
    69121 gggccagggt ggtcaggcat ttgtgcgcgg tattaacgac agcggggaat taacagtgcg
    69181 ctggtatgaa aacaaccaaa ccgttgactg taagttgcac taccagttcc cggcgcagcc
    69241 acaaacgctg ggaagtacca acaccttatt acttaacaat cttacctgtc aggtagcaaa
    69301 tcactaatat ggaaaattct atgaaaagga tactactaac atccgcgtta ataggtctgg
    69361 gtttaccggc ggttggctca gcaacagatc ttaatgtcga ttttacggcc accgtattag
    69421 ccaccacctg tacgataact atcgtacaag atggtggtcc agtagtgacg ggtagcaacg
    69481 gtgaatatag cctgacaatt cccgatgttg gcctggataa ggttgtcact ggagatgttg
    69541 cagctcaagc caactttaaa ttagtggctt ccgattgcag caatggttat tcaaagattt
    69601 ttaccacact tacaggaaca acgacttccg gtaagttgat tgtcaacgaa gcaagctctg
    69661 gcgctacggg tgtgggtatg gggattaagc gtagagatac cgcagactcg actttcttta
    69721 cacccaataa tactgacaaa tttgaatgga ccgccgatga gaaaactaac ggtgtgcctc
    69781 tgaccgtagc actgcgcgaa acaacggcgg gagcaggacg caccggtgcg tttcaggcaa
    69841 aagccacctt taacttcacc tacgaataac agggacagcc accatgaaat taaaagtcat
    69901 cgctacactg attgctactg ttgccgtggg tgtcagcgtt agcaacaatc tcacttatgc
    69961 gagtacgaca tccgcctctt taaccgtaaa cagtaacctg actatgggta cctgtagtgc
    70021 gaaggtagtg gataccagtg gtgccgtgat cgacaccgtt gtcttgggca atgtttatat
    70081 ttctgaactg gtagccaaaa gcaaggtaca accatttaaa atccgcttca gcgattgtgc
    70141 cggccttcca ggaaagagtg ctcaactcgt cttagcgccc agagcaaatg tcggttgtgc
    70201 aggcggcatg tctaacactg cagccttttc aaatcaactc aactccacca ccgatggtgg
    70261 ttcaagcaga gcagcagtcg aagtatggac gactacaagc cccgagggaa atggcagtga
    70321 gcaattcaat tgttatcagc gaaacgtcat caatgtcgat ctttctaatg ccagcacaac
    70381 acagccttat gattttgatt taagcgcacg aatgattatc gctgaaggat ggtcggtcaa
    70441 tgacgtatta ccgggtagat tcctctctcc tacgactttc acaatcactt atcagtaata
    70501 gcggataagg atattagaat gctatgcagg catcataaat ttgtacgttt tttggggctg
    70561 gcaacagccc tcattacgcc ttttgcctat gctggccagg atgtggatct cacggcgcag
    70621 attgtggcca gcacctgtca ggttgaagtc agtaataatg gcgttgtcga tctcggcaca
    70681 gtgacgctgg actattttgc cgacaatgta accccgacaa cggattatgc gggtggcaaa
    70741 acgtttaatg tgaatgtggt ctcttgcgat aacattcaaa cgacacagtc gcaaatgaaa
    70801 ttggattttc agccgcaggc gggatcgctt gcacaggtga ataatcaaat ttttagtaat
    70861 gaatacgaac agcaggctac cggggcgaaa aacgtcggca ttgtgatatt ttcggcccaa
    70921 ccgaatcagc agacatttaa cgtgcgagga acggatggtt cctcgacagc aatttattcg
    70981 gtcgcgcctg gcaatgctgt tccctcaacc tggacctttt attcccgtat gcagcgggtg
    71041 aataatgccc ttcctcctga atcgggaatg gtgagaagcc aggttattgt taacgtatct
    71101 tacgaataat aaggataatt atgcaaagga aaggcaataa actgttgatt cagttatgca
    71161 gtgcgatact gctatttttt accacatcct ggtatgcatt ggcgaatgaa tgttatatag
    71221 agagaaatgc tgaagggcct tatgatatgg tgataaaccc aaagcccttc agtatacagt
    71281 cagcgatggt cacagtcccg gaagaaatag gcttcgcgac ctgggattct tatattgatc
    71341 ttcggggcga taagattggt tgtaaatcct taggtagtaa cacaagctca gtgcattttc
    71401 ttaatactgc tgatgccagt ctcttatcaa cctatactac cagccatggc gatgctttat
    71461 tgaaaacaac cgttcccggt attgtttatt cagtagaatt agtatgtaca agttgtggag
    71521 ccgcggagga gcttgatttg tatcttcctg cacaaagtgg tgcagataat catacagata
    71581 acgccagctc aaagtgggcc tatgagtata gtgacgagag ctggtattta cgatttcgtt
    71641 ttttctggac cccggaattt aaacctcaaa atggggtttc agaaggaact gcgataccag
    71701 gtaaaatagc ttcctggtat ataggcaata atgaccaacc atggataaat tttttcgttg
    71761 atacaagctc cgttcatttt tacgttgacg aaccgacctg tgcaacagtt gccctcgcac
    71821 aagatcaggg caacgtcagt ggcaaccagg taacgcttgg gaacagctat gtttcggaag
    71881 tgaaaaatgg gcttacgcgg gaaatccctt tttctatccg tgctgaatac tgctatgcca
    71941 gtaaaattac ggttaagttg aaagcggcaa ataaacccag cgacgccaca ctagtaggta
    72001 aaacgaatgg ctcggcttca ggcgtggctg taaaagtaaa ttcaacttat gacaatagca
    72061 aagtattgtt aaaagcagat ggtagcaaca cggttgacta taacttcgcc gtctggtcaa
    72121 acaacctgct gcttttacct tttacggcgc agctggtacc ggatggcagt ggtgctgctg
    72181 tcggtgttgg aaccttctct ggtaacgcga ccttctcctt tacctacgaa taagtaaaac
    72241 gtcattcctt cctcatcgca ccagagttct gctctggtgc gaacgttttc cccacactaa
    72301 aaaccattgc ccccctgatt tcgactgtta gaatgcgatc aatctgactt tagccatcag
    72361 ggtacgttat gaaaccgacc accattgctt cattgcaaaa atgcaaacag gaaaaaaaac
    72421 gtttcgcgac catcaccgct tacgactata gcttcgccaa actctttgct gacgaagggc
    72481 tgaacgtgat gctggtgggt gattcgctgg gcatgaccgt tcagggccac gactccactc
    72541 tgccagtaac cgttgccgat atcgcctacc acactgccgc cgtacgtcgc ggcgcgccaa
    72601 actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
    72661 aaaacgccgc aacggttatg cgtgccggtg ctaacatggt caaaattgaa ggcggtgagt
    72721 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
    72781 gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg
    72841 aagcgagcga tcgactgctt agcgatgcat tagccttaga agccgctggt gcacagttgc
    72901 tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca ctggtgatcc
    72961 cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgatg
    73021 cgttcggcat taccggcggc catatcccta aatttgccaa aaatttcctc gctgaaacgg
    73081 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
    73141 gcgaagaaca cagtttccat taaggagtga cgttgtgtta attatcgaaa ccctgccact
    73201 gctgcgccag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtacctac
    73261 catgggcaac ctgcatgatg gccacatgaa gctggtcgac gaagccaaag cccgcgccga
    73321 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aggacctggc
    73381 tcgttaccca cgcaccttgc aggaagactg cgaaaagctg aacaagcgca aagtggatct
    73441 ggttttcgcg ccctcggtaa aagagatcta cccgaacggt actgaaaccc atacttacgt
    73501 tgacgttcca gggctttcga ccatgctgga aggcgccagc cgtccggggc attttcgcgg
    73561 cgtgtcgact atcgtcagca agctgtttaa cctggtccag ccggacatcg cctgcttcgg
    73621 tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg gttgctgata tgggctttga
    73681 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcac tgagttcccg
    73741 taacggttat ctgacggcag aacaacgcaa aattgcgccc ggtctgtaca aagttttaag
    73801 ttcgattgcc gacaaattgc aggctggcga acgggatctc gatgaaatta ttgctattgc
    73861 cgggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
    73921 cacgttgcag gaggtctctg aaaacagcaa acgggccgta attctggtag ccgcctggct
    73981 tggcgatgct cgcctgatcg acaacaagat ggtcgaactg gcgtaatact taactggcgc
    74041 tacggctgat ggcgccagtt attaatttgc cctacgtcat ggcgatgacg tctgtaagtg
    74101 attattcata tcgctggcaa tttagttaga gttaattagc ttatcaatct ctgtaagagg
    74161 taaagttgtc acctctgcaa cgtcttcccg agacatccct ttacttagaa aacgctgggc
    74221 gaattcctgt cttaccttct ggagttcctc ttgatatccc ttctgaaatc ccttctcttc
    74281 aaaccactgc gccaacgtct gcctggtttc tctccgtgtc tttatttcca taggttccct
    74341 taattatctg gtcagttaac aataacaatt atctggctat aactgatgat gcagacaact
    74401 tagttaatca gcttatcaat ctcggcaagg ggtaaatttg ccatctctgc aacgtcttcc
    74461 cgagacattc ctttattcag aagacgcagg acgaattctt gcctttcctc ctgtcttccc
    74521 ctctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc cctgtctctc
    74581 agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat atagttttgc
    74641 atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc
    74701 agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag aatcgcaatc
    74761 cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag cgggaaagga
    74821 ctgttataga cgcgtcgcgc cagctcaggc gagtaaaaca tatcaaacca gcacattgag
    74881 agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag cggcagctta
    74941 tcgtggtcag cctccagatg ccggtgcatg gcggcaatag aataacgcat catgcgaaaa
    75001 gccattttct tatctggctt gctttgatgt tcaatcacta catgcagata accgggattg
    75061 ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct ctcttcaatg
    75121 aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa ttccactggc
    75181 aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca ttaaaaattg tttaaatacc
    75241 gcgtcatgcg gtgtggtact cggtgcatcc atcttccctg tcttccgtgt tgtcttgcgt
    75301 gaaggcagta tccagtcaag gcgtgctatc gtcagccacc tctttagtgt ttaccgagca
    75361 gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg cgcggtacgc
    75421 aaactgctga tgcaaatgcc gtcagatgag tagacactaa acaaaaatcg ggcaatactg
    75481 cgtgagaatt tcctcaaagc aggcaccgcc tgcttcgtta acgacagggt agaaaggtag
    75541 aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg actcatgcgg
    75601 acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca gccggtattc
    75661 tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc tccacttatg
    75721 ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg gcccactgcg
    75781 ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat gaagaagctc
    75841 gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga
    75901 aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact caccagccgc
    75961 gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa caacggatta
    76021 tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt
    76081 gctgccttca cggctttgtc attaaagccg ccaaacggat acgaaagata ccagacatgc
    76141 ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat attgtgctca
    76201 ctacggctca gtaatatggg tcggcgataa ccatctaccc gatgcaaaaa atgggtatgt
    76261 gactggaaat caaatacatc gcgaatttcg ttaagctcag aaacgctcat aaattgcagc
    76321 gattttgggt tccacttctg cgggtgacgt ttgatgcgtg aggtaacaat aaacgccgtc
    76381 gccttcatgc catattgttt caacacagga tacgcatagc ggctcaccga cttgaggcca
    76441 tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt cacgtaacct
    76501 tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc catctggtta
    76561 ttgaaagcgc gtaccgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcgtcg
    76621 cgcagaatat ggtgataagt tagcaccgga aggccattat cgggttgggc atccagcgcg
    76681 ctgatatagg ccagtcgatc gccaatacgg acctgatacc aggtttgatt taacctgtct
    76741 ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactg
    76801 cccgcactcg gcgcgttata gaccggcgta tctttccagg taactaagtt ctgattactc
    76861 agcggcttgt tgaggtcacc caaaccgtct tcaacttttt gtcgcccctg aaccggctcg
    76921 agatgccctt tatcgataaa ccctttacca aagccaaaat taaatgcgta ataacttgcg
    76981 gcagtgggct ccacggcaat gatttgtccg gcccgaatat tccccacggt caccatcttg
    77041 tcaccaattt gcgcccagac cgcagcattt tcgatggttt gcatataacg ggcaggtaac
    77101 gcggcactga cacttgcggt aaacagcatc agcaggagaa taacagcttg tttgtacatg
    77161 aaaaaataac cgagggtgaa acaacgcttt tattgtagca aaagcgctat aagaatcgtg
    77221 ggttaatact catacaaatt atgtttgagg acgaaaggcg gatttttttg ttgttggtgc
    77281 caaagactac tcacctccgg cgcacccagt tcgacaatac gctcgcgaaa ctctgaactg
    77341 gtcatggttt cacggcaagc catcatctgt tcaattaacg gtaacgtgac accagaaatc
    77401 acttcgcaac gggagtgttt gtggcttaat aatgaagcca cgcgatacgg tggcgctccg
    77461 gctatatcgg ttaagaagat gacaccctca ccagagtccg cttcatgcag agcatcgcac
    77521 atcatgcggc tgagcatatt agagcttaac ccacgccaga aattcacggc ccggcactga
    77581 agaagcgccc catgtttttt ctccagcgca tccagtatct cttgcgcccg gtcatcgtga
    77641 caggtaatta cccaacctaa catcgccttc ctccttagcc gaaaatcagt ctaccctgac
    77701 cattgttgat aaagggtgat agccgtcaaa actcctctcc cccaaatcca gaggagagga
    77761 aatagcctta gctacgcaaa ccacgtccac gttggatcag cgaccaacag atcaaataaa
    77821 acgccacaat aaagaccacc agtacgccaa aggtagtgac cagcggaaca tcattgatac
    77881 cgaggaagcc gtagcggaaa ccactgatca tataaacgat tgggttcagg tgcgacagcc
    77941 cttgccagaa cggcggcaac aaagtcaggg agtaaaagac cccacccaaa tacgtgagtg
    78001 gcgttaacac aaaggttggc accaggctga tgtcatcgaa cgttttggca aacacaccgt
    78061 tcagcaaacc cgcaagggag aacaacaccg ccgtgagcac cagggttaag gcaacgaata
    78121 cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa
    78181 caaacagacc acgcgccacg ccaccgccga catatccggc aataatgacg tgagtcggaa
    78241 ccggcgctac cagcagctct tcaatattac gctggaattt ggcaccaaaa aatgatgacg
    78301 cgacgttggc gtaggcgttg gtgatcaccg acatcatgat cagccccggt acgatgaact
    78361 gcatgtagct gaagccatgc atatcgccaa tacgcgaacc aatcaggtta ccgaaaataa
    78421 taaagtaaag ggtcatggtg ataactggcg gcaccagcgt ctgcacccag atacgcataa
    78481 agcgatggat ctctttcgcc cagatgcttt ttaacgccac ccagtaaaga tgcatcatgc
    78541 gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac ggttagcttt
    78601 gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata cgctgttgat
    78661 cccctgctca cgcagcactt caacttccag cgtcgcggta tcgaccagtc gatactgata
    78721 gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg agaataaagg tttccgattt
    78781 cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccgt gttgaataat
    78841 gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtggtga gaatgatggt
    78901 ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc
    78961 cacgcctgca gtcggctcgt cgagaatcag tagtttaggt tcatgcatta acgcacgggc
    79021 aatcattaaa cggcgcttca tcccgccaga taacatgcgc gcacgttcgt tgcgttttcc
    79081 ccataaatcg agctgcttaa gatatttttc gctgcggatg tacgcctctt tgcgctccac
    79141 accgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt
    79201 aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatctt tctcgagatc
    79261 gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga taataccgat
    79321 agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac ccgcttcgac
    79381 ctgcaaatct attccacgaa gcgcctgaac gccgcctgga taggtttttt taagctgttg
    79441 aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac tttatctgtg
    79501 gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg ttacaggtcg
    79561 ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg gtcaaaaatg
    79621 ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa accgcgcttt
    79681 ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg
    79741 ggcgaactct ttgttcaccg taatgttgct aacctggtca ttcacaccga cctgaactgc
    79801 ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg aacacattat tatctgtggc
    79861 cactacggtt gcggcggcgt acaagccgca gttgaaaacc cggaactggg gcttatcaac
    79921 aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct cggcgaaatg
    79981 ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca ggtgtataac
    80041 ctgggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt taccattcac
    80101 ggctgggcct atggcattca cgacggcttg ctgcgtgatc tggatgttac tgccaccaac
    80161 cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct gaaacacgcc
    80221 aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac acttactcgt
    80281 ccagcagaat cactttgccg atatacggca gatgacgata acgctgtgcg taatcaatac
    80341 cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattctacc gggacgttca
    80401 cttcacggcg ggacggttta tccagcagcg tacaaatcgc cagcgacttc ggttcgcgca
    80461 ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata tcttcaacaa
    80521 tcagcacgtc cttgccacgg atatcttcat ccagatcttt gaggattttc acatcacggg
    80581 tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca tgagatacct
    80641 gaacttcacg gcacaggtcc gccataaaca taaatgagcc acgcagcaga cccaccagca
    80701 ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt tcggcgatac
    80761 gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcata tctctaacca
    80821 tatgatttaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag
    80881 acacgcagta taccagtaaa actattcatt cgcggaatct gttaataaag ctcgaattgt
    80941 gatgacggtc acacatgtta aaacctataa ttaattgcta ctaaaatatt aatgaattga
    81001 aatggtgtct ctttatggca attaacaata caggctcgcg acgattactc gtcacgctaa
    81061 cagccctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc tggctggtcg
    81121 cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc gtcgcctgga
    81181 tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc cgccctgctg ctcggcacca
    81241 tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg cgcagcgata
    81301 ttctggtctt cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc
    81361 ctgccagcgg cgcagttgct gcgctggtgg tcgcactgct gattagcggt ggtatcctga
    81421 cctgggccgg atttaacgat ccgcaggaaa tcaacggcac cttaagcgcc gatgccacac
    81481 ctgccgaagc tatctcaccc gttgccgatc aggactggcc tgcgtatggt cgtaatcagg
    81541 aaggtcaacg ctattcgccc ctgaaacaga tcaacgccga taacgtccat aacctgaaag
    81601 aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggt gaaatcacca
    81661 atgaagtgac gccgattaaa gtgggcgaca ccctttacct gtgtaccgct caccagcgac
    81721 tgtttgcgct tgatgccgcc agcggcaaag agaaatggca ttacgatcct gagctgaaaa
    81781 ccaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag
    81841 aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt ccggtcaatg
    81901 atggtcgact gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata
    81961 aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg tatgaaccga
    82021 cttcgccacc gattatcacc gataaaacca ttgtgatggc aggttcagtt accgataact
    82081 tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc
    82141 tgtgggcttt tgatccgggc gcgaaagatc cgaacgcaat cccgtctgac gaacacacct
    82201 ttacctttaa ctcgccaaac tcgtgggcac cagcggccta tgacgcgaag ctggatctgg
    82261 tgtatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc acaccggaac
    82321 aggaacgtta tgccagctcg attctggcgc tgaatgccac aaccgggaaa ttggcgtgga
    82381 gctaccagac cgttcaccac gacctgtggg atatggatct tccggcacag ccgacgctgg
    82441 cggacatcac cgttaatggt cagaaagtgc cagttattta cgctccggcg aaaaccggca
    82501 acatttttgt gctcgatcgt cgtaatggcg aactggtggt tccggcaccg gaaaaaccgg
    82561 ttccccaagg tgctgccaaa ggcgattacg ttacccctac tcaaccattc tcggaactga
    82621 gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg gggagccacc atgtttgacc
    82681 aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc acgccgccat
    82741 ctgaacaggg cacgctggtc ttcccgggta acctgggaat gtttgaatgg ggcgggattt
    82801 ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg tttgtttcga
    82861 aactgatccc acgcggtccg ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg
    82921 gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt
    82981 tcctctcacc atttggtctg ccatgtaaac agccagcatg gggttatatc tcggcgctgg
    83041 atctgaaaac caatgaagtg gtgtggaaga aacgtattgg tacgccgcag gacagtatgc
    83101 cgttcccgat gcctgtaccg gtgccgttca atatgggtat gccgatgctg ggcgggccaa
    83161 tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac ctgcgcgctt
    83221 acaacatgag caacggtgaa aaactgtggc agggtcgttt accagcgggt ggtcaggcga
    83281 cgccaatgac ctatgaagtg aatggtaagc agtatgtggt gatctccgca ggcggtcacg
    83341 gttcatttgg tacgaagatg ggcgactata ttgtggctta tgccctgccg gatgatgtga
    83401 agtaagactt gctcagattg ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa
    83461 cgccttatct agcctacaaa attgtgcaaa atcaatggat tgcacaggta gcgtaggcct
    83521 gataagcgta gcgcatcagg cagatttgcg tttgtcatca gtttaatgcc cggagagatc
    83581 cgggcatatt cccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    83641 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc gtcgtgatta
    83701 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    83761 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcgt gccgtgaata
    83821 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    83881 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    83941 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    84001 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    84061 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    84121 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtctggcaa agcaccggag
    84181 gcactaatag caatcggctg aatccgcatt accggatgcg gcttgtcaaa cggtgcaatc
    84241 gccatcccca tctggctgac cggcagcgtc accaggtcaa agggtttgtt gtcgttaacc
    84301 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgttcac cttcaccggt
    84361 tcaggtagca gaccaccgtc gctggcaatc acataaagcg ggcgattgtc gctggtggcg
    84421 aaattgagcg agcgggcatt acagccattg agcaaacgca ggcgcagcca accacgcggg
    84481 gcagcgtgtt gcgggtagat tgcaccgttg gtcagcaacg tatcgccaaa ccagcccacg
    84541 gcggcggtca tcacatccag ttgataatca atctgcccgt cggcgctaaa tttcttatcc
    84601 tgaacgatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    84661 tcgtcatctt caatcaccac cagaccagcc agccccatcg ccacctgtcg cccggtcttg
    84721 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    84781 gagcgcttgc cacctggcgg aataattccc tgtgggccgc cgtcgacttc acccggtact
    84841 tccagcccgt gccagtgcaa cgtcgtctct tccgtcagtt ggttatagat atcaaccgtt
    84901 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    84961 gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc caatagttaa ctgaatgcga
    85021 ttacgagcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    85081 actgcacggc tccacagtgg caaggctgaa gccacaccca gtgcgacgga atatttcaag
    85141 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    85201 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    85261 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    85321 caatgaagac gtttttcaga acagttttat tcggcagcct gatggccgtt tgcgcaaaca
    85381 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    85441 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    85501 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    85561 ccctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaagt
    85621 gcaaagccct ggctcgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    85681 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    85741 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    85801 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    85861 agaccgatca ccaggatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    85921 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    85981 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    86041 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    86101 tcgatgcggg tgtcgtatcg ttctgccgtc agtatctacc caaccataac gccggtagct
    86161 acgacgatcc gcgctttaag ctggtgatcg atgatggcgt caatttcgtt aatcaaacca
    86221 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagcc
    86281 ttttcacttc ggcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    86341 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc catcgcaaac
    86401 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    86461 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    86521 ttattcaggc gcgttttctc gcctctggcc tgaaatgccg ttattacaat ccggcaatcc
    86581 atacggcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    86641 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    86701 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    86761 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    86821 cctgttccat tatcggggct aatattctta acatcgcccg ccaggattac gaaccacagg
    86881 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    86941 cagaacaccc cggcccactg ccagaaacgg tcgttgccca tcttgataaa agtcatattt
    87001 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    87061 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    87121 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttacc cgcgacatta
    87181 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    87241 atatgaaggc gctgtatgac atggtggatg tgaacgtcta tcaggaaaat atcttccata
    87301 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    87361 acttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    87421 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    87481 cagatttatt tacttctgct gcacgaaatt acggtaagcc gccacgacct gcagaaaatc
    87541 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatcccct cttccatttc
    87601 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag
    87661 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    87721 ttgttccact tcatcaagca aggccaggtt ttctttcacc tcttcattaa accagtgccc
    87781 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtggtat cgcgcagaaa
    87841 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgccat
    87901 tgatgtaatt atcgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    87961 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    88021 gactttttaa actgcagtct ggaaaatcac accatcggct ttttcagtgt actgagaaag
    88081 ctggttgaag ttcaggtaac ggtaagtatc aacggcagtt ttatccacct gcgccacgta
    88141 ggtctggtac tcttccggcg tcggtagttt gccaatcagc gccgcaacag cagccagttc
    88201 cgcagaagcc aggaagacat tcgcgccagt ccccagacgg ttcgggaagt tacgggtaga
    88261 ggtggaaacc accgttgcac cgtccgccac acgcgcctgg ttacccatac acagggaaca
    88321 gccagggatc tcgatacgcg caccactctt accgaagacg ctgtagtagc cttcttcggt
    88381 caactgtgcg gcgtccatac gggttggcgg tgccacccac aggcgggtcg gcaactggcc
    88441 tttatgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttgg tcatgcagga
    88501 accgataaac acttcgtcga tcttctcacc ctgtaccgca gacagcggac gcgcgtcatc
    88561 cgggtcgttc ggagcacaca ggattggctc tttaatatcc gccagatcga tgtcgatcac
    88621 tgccgcgtat tccgcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    88681 catgccctga atacgacgtt ccagggtacg acgatcgccg taaccttccg cgatcatcca
    88741 cttcagcagg acgatattag agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    88801 ggtacaaccg gcagcagaac gctcggcgga cgcatcggtc agttcaaatg cctgctcaac
    88861 tttcagatcc ggcagacctt caatttccag aatgcgacct gagaagatgt ttttcttgcc
    88921 tttcttctca acggtcagca gaccttgttt gatcgcatac agcggaatag cgtgtaccag
    88981 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    89041 catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg
    89101 aaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    89161 cagcagcata cggttcagcc aggagtggat tacgccgtca cccggacgca gcgacacacc
    89221 gccacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ccggcttcgg
    89281 atacgccgct gtgtggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    89341 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgggaac ctacagaagt
    89401 cattttcggt tcacagtacg cgcccggacg aatgcctttc acgccacagg cacggcctac
    89461 cattttctgc gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    89521 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    89581 aatcagcgga atacggccgc cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    89641 gaaggtcgcc agcagttcgc ctgtttcgtg gttacgcact tcacctttgt acgggtaaac
    89701 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    89761 gtcttccatc gtgttaaaga agattggtgc aattttaccg ccgaggcaca aaccaccgcc
    89821 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttggtggc
    89881 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    89941 ctgttgcaga gcttcaatct gtttgatcgg gccaacaaca cctggctggt ctggctcaat
    90001 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgcgacca
    90061 cgcatccggt gccggagaaa ggtcatcggt gttggtttcg ccagtgactt tgaagacggt
    90121 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    90181 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
    90241 gttatcgaac atcagcagcg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
    90301 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tgcccagcag
    90361 ttcgatggct ttttccggag tcagcagtgg ggatttggct tcacctttcg cgacagcagc
    90421 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
    90481 tagatctaac aggaattctt tttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    90541 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    90601 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    90661 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    90721 acgggcagaa tagcaggatt ttgacgatgt gttaatctgt ttacaaaaaa gcaacataaa
    90781 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    90841 attcgcatag tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    90901 ccgcctcttt atactcagat ttacagcacc tgcgggtggc agttcgccga ccattgcgat
    90961 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta
    91021 atttgcagtg ccgggctttg tgccgcctct gccgggctat atataaaaag ccgcacagtg
    91081 gaagcgcctg tggaaccgca atcgacacaa cagactgcgc ctgacatcac cgcagttacg
    91141 cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgttaaatc cgcattcagc
    91201 actgcacaaa tcgatcaatg ggttgcacct gtcgcgctat accccgactc tctgctttca
    91261 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    91321 gataatccac ttaaacaagg cgatgctgct attctggcgg tatctgacca gccgtgggac
    91381 gctagcgtca aatcactggt ggcctttcca caattgatgg cgttgatggg cgaaaacccg
    91441 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    91501 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    91561 aaagttatta ccacaacgaa gaaaaccgta ccggtaacac agacagtcac ggctcccgtc
    91621 ataccatcca ataccgtttc aactgccaac cccgtcatta cagagcctgc aacaaccgtc
    91681 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    91741 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    91801 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    91861 tacgcactat tcagcagcat cgactgggat gacgacgatc atgaccatca tcatgacgat
    91921 gattatcatc accacgatgg cggtcatcgt gacggtaatg gctggcaaca caacggcgac
    91981 aacatcaata tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct tactgataag
    92041 aatatggcat ggcggcacaa tccaaactac cgtaatggtg tgccctatca tgatcaggat
    92101 atggcaaagc ggtttcatca aaccgatgtc aacggcggaa tgagtgccac gcagctacct
    92161 gctccaacac gcgatagcca gcgtcaggcg gcagcaagtc agtttcagca acgaacacac
    92221 gccgcccccg tcattacacg agatacccaa cgtcaggcag cggcacagcg gtttaatgaa
    92281 gctgaacact atgggagcta tgacgacttc cgcgacttca gccgtcgcca accactgacc
    92341 cagcaacaaa aggacgccgc tcgtcagcgt tatcagtcag cttctcctga gccgcgccag
    92401 gcagttcgcg agagaatgca gactaacccg cagaaccagc agcgaagaga ggcagcgcgt
    92461 gagcgcattc agcccgcctc gcctgagcag cgccaggcag tccgcgagaa aatgcagact
    92521 aacccacaga tccagcagcg aagagaggca gcgcgtgagc gtattcagtc agcctcgcct
    92581 gagcagcgcc aggtgtttaa ggaaaaagta cagcagagcc cactgaacca acagcaacgt
    92641 gataacgccc gccagcgtgt tcaatcggca tcacctgaac aacgtcaggt ttttcgggag
    92701 aaagttaagg agagccaccc acaacgtcta aacgacagta accatactgc caggctaaat
    92761 aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgactggaa
    92821 aggtaaatta cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt
    92881 tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac
    92941 cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcatggatgg
    93001 tcagcgcgat gtcttcagcg tcacaaccca tttcgattgc caggccgatt tcacccagca
    93061 gctcgccgcc gttggtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga
    93121 aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc
    93181 acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac
    93241 ctacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc ggatcgaagt
    93301 agtgtttctt accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg
    93361 ccagcatcgg ctgaccgacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca
    93421 gctgtttgtc aacgcggatg aaaccacgat cgtcaacttc cacgccagct ttgcctgcgt
    93481 cgaggttttt accgttcggc acacgaccaa tcgctaccag cactgcgtcg taacgctgcg
    93541 gttcagctgg tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa
    93601 cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga
    93661 ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa
    93721 tctgtgaacc cagcgcgtgg taaacggtgc ccatttccag accgatgata ccgccaccca
    93781 ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac
    93841 gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg
    93901 cgttgtcgaa gttgatcacg gttttaccgt tctcaccttc aacttccagg gtgttagccc
    93961 cggtaaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca
    94021 gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg tcgatatcgg
    94081 ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt
    94141 ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa
    94201 gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag
    94261 cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca
    94321 tcatgacctc tatatattta tctccggcgg gtctgacgtt ttttttatga cgcccattat
    94381 ccaccgggtc gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa
    94441 acggcaaccg atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa
    94501 aagccggccg ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg
    94561 tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga
    94621 aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct
    94681 ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca atcggcgcga
    94741 agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc
    94801 ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct
    94861 cgatgatgcc tttcttgttg acgtctttga ataccggaac aaccaaaccg ttcggggtat
    94921 ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca
    94981 gcgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga
    95041 caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac
    95101 ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg agtaacatgc gggatcatca
    95161 cccagttacg gctcaggttc gcaccagaaa ttttctggat acggcccagt tccacttctt
    95221 cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgcct gggataccgc
    95281 cgccagtcgc cgccggagct gcttctgcac gtttgatagc ttctttcacg taagcctgaa
    95341 cgtcttcgcg caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc
    95401 caaactcgcg tgccagacgg cggatcagcg gagtcgcgtg aacataagcg tcgttttcag
    95461 caaattcaga tttgccttcc gctttcgcag ctggtgctgc tgccggggct tcagcttttg
    95521 ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt
    95581 caacttcgaa gatcataatc agcgagccag ttttcacttt atcgccaacg ttgactttca
    95641 gttccttcac gacgcctgca aacggagccg gaacttccat agaagctttg tcgccttcta
    95701 cggtaatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca
    95761 cttcaacttc gtcaccgccg atatccggaa cgttaacttc tttcacgcca gctgctggtg
    95821 caggggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac
    95881 ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcgccg gtgttcactt
    95941 tgatttcttt cacggtgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt
    96001 ctacggtgat cagcgactgc tcagcttcaa ctttgtcgcc aactttcacc aggatttcgg
    96061 tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gcggcagccg
    96121 cgggtgctgc tgccggagct gcttctttct tctcttctgc ctgagcaggt gcagcgtctg
    96181 ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa
    96241 cagagacttt gatctcttta acgatacccg cctgcggaga cggaacttcc atagaggctt
    96301 tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca
    96361 ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca
    96421 ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt
    96481 ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag
    96541 cgccgcaacc acgacataag aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct
    96601 gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt
    96661 acggacctgc tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt
    96721 catcacctgc gcgatatacg gaacgcgcgg agtttccagt gggtgcagca tgttccagcg
    96781 ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag gtcacgctat aaacgtcaga
    96841 acctacgccg taatctttcg ccaggatctc tgccgcttca cggacgtgac gcaggataga
    96901 accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat
    96961 acctttacgg ataccttcct cagcaccttc cggcattgcc ggcatgtggt agttttcgtt
    97021 cagcgtggtg atgtagtagt aaacgttctc ttgtttttca ccgtacatac gctccagacc
    97081 gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg
    97141 aatagtcagc gactgaatat ggctgtgacc atcttcgtgc tgcagacctt caccgttcag
    97201 ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt cgccagccgc
    97261 ccagcacaga tcgccgatac gctggaagcc gaacatcgag taatagatgt agaacggaat
    97321 catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc
    97381 cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac
    97441 ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg
    97501 gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc
    97561 tttgatcgac ttgttcttca gcatcacgtt cagggcacga acgaaagcga tagtggtaga
    97621 gatctctttg ctttgctctt ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag
    97681 cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgagc
    97741 gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca gtttttcgat
    97801 atctgcatca gacaccggca cattgaaacg gtcgcggata tgacgcacac cgtccatgtt
    97861 cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc
    97921 tttaatggta tgagcaagga ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa
    97981 tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga tctgctcgtc
    98041 agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac
    98101 gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag
    98161 ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat
    98221 cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt
    98281 gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaagaccag
    98341 gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat
    98401 ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag gtatctttca ggccacggtg
    98461 ttccagatat ttcaggaatt tagcctggta aatagcacca atcggaccca gacccataga
    98521 tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc
    98581 attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag
    98641 gaaagcacga gcgtaaacgc ccggggagat gtggccctgg aagtaaacca agtcgccgcc
    98701 atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga
    98761 agactggaag gacgccatgt ggccgcccag ttcgaggtct tttttcgacg cacgcagaac
    98821 cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc
    98881 cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga taccggtgcc
    98941 tgcggctacg ttgacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg
    99001 agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt
    99061 ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta
    99121 ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac
    99181 aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata
    99241 atttttacca gaaaaatcac taattctttc gttgctccag acgacgcaga gaacgctcac
    99301 ggcggctctc ttcacggctt ctgtcgagca aaatttcttc gataaaggcc agatggcgat
    99361 gcgatgcttc gcgcgcttct tccggcttac cggccataat tgcttcgaat atacgggtgc
    99421 ggtgactact caccagcggc agcatctcgc gacgcgcata gagcaattcg aagttctgac
    99481 ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaaa accacattgt
    99541 gggctgcttc ggtgacggca atctgatact ggagtacggc gtttgattcc gcgtccagat
    99601 cgccagactg ctgcgccagt tctatggcgt ggtggagttc acggatgcgt tccttgtctt
    99661 catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt
    99721 cgagcaagtc atactgtgat tcaggatggt cggagagcag ctctaccagc ggatcgctga
    99781 agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc
    99841 ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg tcaaactgtt
    99901 ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca
    99961 aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg
   100021 ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct
   100081 gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg
   100141 aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta actatgcaca
   100201 aatgtagact ttacgtagga aagggatttt ttaacgatta ttaaactata aaaatccgaa
   100261 ttga
//
LOCUS       DAZGCQ010000003        89963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000003 DAZGCQ010000000
VERSION     DAZGCQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.003"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..1906)
                     /gene="gss"
                     /locus_tag="ACHK07_000198"
     CDS             complement(47..1906)
                     /gene="gss"
                     /locus_tag="ACHK07_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO8100594.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2111..2977
                     /gene="yghU"
                     /locus_tag="ACHK07_000199"
     CDS             2111..2977
                     /gene="yghU"
                     /locus_tag="ACHK07_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO8100595.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="ACHK07_000200"
     CDS             complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="ACHK07_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO8100596.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3361..3702)
                     /gene="hypA"
                     /locus_tag="ACHK07_000201"
     CDS             complement(3361..3702)
                     /gene="hypA"
                     /locus_tag="ACHK07_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8100597.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIIYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3695..4183)
                     /gene="hybE"
                     /locus_tag="ACHK07_000202"
     CDS             complement(3695..4183)
                     /gene="hybE"
                     /locus_tag="ACHK07_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO8100598.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPLMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4176..4670)
                     /gene="hybD"
                     /locus_tag="ACHK07_000203"
     CDS             complement(4176..4670)
                     /gene="hybD"
                     /locus_tag="ACHK07_000203"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO8100599.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4670..6373)
                     /gene="hybC"
                     /locus_tag="ACHK07_000204"
     CDS             complement(4670..6373)
                     /gene="hybC"
                     /locus_tag="ACHK07_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO8100600.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6370..7548)
                     /gene="hybB"
                     /locus_tag="ACHK07_000205"
     CDS             complement(6370..7548)
                     /gene="hybB"
                     /locus_tag="ACHK07_000205"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO8100601.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7538..8524)
                     /gene="hybA"
                     /locus_tag="ACHK07_000206"
     CDS             complement(7538..8524)
                     /gene="hybA"
                     /locus_tag="ACHK07_000206"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO8100602.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8527..9645)
                     /gene="hybO"
                     /locus_tag="ACHK07_000207"
     CDS             complement(8527..9645)
                     /gene="hybO"
                     /locus_tag="ACHK07_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO8100603.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVIGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9834..10121)
                     /gene="yghW"
                     /locus_tag="ACHK07_000208"
     CDS             complement(9834..10121)
                     /gene="yghW"
                     /locus_tag="ACHK07_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO8100604.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10240..11127)
                     /gene="yghX"
                     /locus_tag="ACHK07_000209"
     CDS             complement(10240..11127)
                     /gene="yghX"
                     /locus_tag="ACHK07_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO8100605.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11284..12324
                     /gene="gpr"
                     /locus_tag="ACHK07_000210"
     CDS             11284..12324
                     /gene="gpr"
                     /locus_tag="ACHK07_000210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO8100606.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12364..12858)
                     /gene="yqhA"
                     /locus_tag="ACHK07_000211"
     CDS             complement(12364..12858)
                     /gene="yqhA"
                     /locus_tag="ACHK07_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO8100607.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13049..13933
                     /gene="yghA"
                     /locus_tag="ACHK07_000212"
     CDS             13049..13933
                     /gene="yghA"
                     /locus_tag="ACHK07_000212"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8100608.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIHLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVS
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14205..14630)
                     /gene="exbD"
                     /locus_tag="ACHK07_000213"
     CDS             complement(14205..14630)
                     /gene="exbD"
                     /locus_tag="ACHK07_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO8100609.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14637..15371)
                     /gene="exbB"
                     /locus_tag="ACHK07_000214"
     CDS             complement(14637..15371)
                     /gene="exbB"
                     /locus_tag="ACHK07_000214"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8100610.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15623..16810
                     /gene="metC"
                     /locus_tag="ACHK07_000215"
     CDS             15623..16810
                     /gene="metC"
                     /locus_tag="ACHK07_000215"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO8100611.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16950..17609
                     /gene="yghB"
                     /locus_tag="ACHK07_000216"
     CDS             16950..17609
                     /gene="yghB"
                     /locus_tag="ACHK07_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO8100612.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17649..18548)
                     /gene="yqhC"
                     /locus_tag="ACHK07_000217"
     CDS             complement(17649..18548)
                     /gene="yqhC"
                     /locus_tag="ACHK07_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO8100613.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18742..19905
                     /gene="yqhD"
                     /locus_tag="ACHK07_000218"
     CDS             18742..19905
                     /gene="yqhD"
                     /locus_tag="ACHK07_000218"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO8100614.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20010..20837
                     /gene="dkgA"
                     /locus_tag="ACHK07_000219"
     CDS             20010..20837
                     /gene="dkgA"
                     /locus_tag="ACHK07_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO8100615.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21037..21963
                     /gene="yqhG"
                     /locus_tag="ACHK07_000220"
     CDS             21037..21963
                     /gene="yqhG"
                     /locus_tag="ACHK07_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO8100616.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSVRIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRIDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     RDGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22012..22269
                     /gene="yqhH"
                     /locus_tag="ACHK07_000221"
     CDS             22012..22269
                     /gene="yqhH"
                     /locus_tag="ACHK07_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO8100617.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22312..24531)
                     /gene="ygiQ"
                     /locus_tag="ACHK07_000222"
     CDS             complement(22312..24531)
                     /gene="ygiQ"
                     /locus_tag="ACHK07_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO8100618.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGGRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24642..26054)
                     /gene="ftsP"
                     /locus_tag="ACHK07_000223"
     CDS             complement(24642..26054)
                     /gene="ftsP"
                     /locus_tag="ACHK07_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO8100619.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26129..26866)
                     /gene="plsC"
                     /locus_tag="ACHK07_000224"
                     /gene_synonym="parF"
     CDS             complement(26129..26866)
                     /gene="plsC"
                     /locus_tag="ACHK07_000224"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO8100620.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27100..29358)
                     /gene="parC"
                     /locus_tag="ACHK07_000225"
     CDS             complement(27100..29358)
                     /gene="parC"
                     /locus_tag="ACHK07_000225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO8100621.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMLRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29496..31103)
                     /gene="ygiS"
                     /locus_tag="ACHK07_000226"
     CDS             complement(29496..31103)
                     /gene="ygiS"
                     /locus_tag="ACHK07_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8100622.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTILQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31212..31694)
                     /gene="ygiV"
                     /locus_tag="ACHK07_000227"
     CDS             complement(31212..31694)
                     /gene="ygiV"
                     /locus_tag="ACHK07_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO8100623.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31747..32139)
                     /gene="ygiW"
                     /locus_tag="ACHK07_000228"
     CDS             complement(31747..32139)
                     /gene="ygiW"
                     /locus_tag="ACHK07_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO8100624.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32291..32950
                     /gene="qseB"
                     /locus_tag="ACHK07_000229"
     CDS             32291..32950
                     /gene="qseB"
                     /locus_tag="ACHK07_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO8100625.1"
                     /translation="MRVLLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALGERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32947..34296
                     /gene="qseC"
                     /locus_tag="ACHK07_000230"
     CDS             32947..34296
                     /gene="qseC"
                     /locus_tag="ACHK07_000230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO8100626.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFVRTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(34342..34674)
                     /gene="ygiZ"
                     /locus_tag="ACHK07_000231"
     CDS             complement(34342..34674)
                     /gene="ygiZ"
                     /locus_tag="ACHK07_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO8100627.1"
                     /translation="MLKHEIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLDSGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVCSFVICLVCGWWLF
                     WGRFIFCY"
     gene            35032..35613
                     /gene="mdaB"
                     /locus_tag="ACHK07_000232"
     CDS             35032..35613
                     /gene="mdaB"
                     /locus_tag="ACHK07_000232"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO8100628.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35644..35958
                     /gene="ygiN"
                     /locus_tag="ACHK07_000233"
     CDS             35644..35958
                     /gene="ygiN"
                     /locus_tag="ACHK07_000233"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO8100629.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36006..37898)
                     /gene="parE"
                     /locus_tag="ACHK07_000234"
     CDS             complement(36006..37898)
                     /gene="parE"
                     /locus_tag="ACHK07_000234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO8100630.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(37927..38508)
                     /gene="yqiA"
                     /locus_tag="ACHK07_000235"
     CDS             complement(37927..38508)
                     /gene="yqiA"
                     /locus_tag="ACHK07_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO8100631.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38508..39335)
                     /gene="cpdA"
                     /locus_tag="ACHK07_000236"
     CDS             complement(38508..39335)
                     /gene="cpdA"
                     /locus_tag="ACHK07_000236"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO8100632.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39360..39782)
                     /gene="yqiB"
                     /locus_tag="ACHK07_000237"
     CDS             complement(39360..39782)
                     /gene="yqiB"
                     /locus_tag="ACHK07_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO8100633.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39783..40412)
                     /gene="nudF"
                     /locus_tag="ACHK07_000238"
     CDS             complement(39783..40412)
                     /gene="nudF"
                     /locus_tag="ACHK07_000238"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO8100634.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWV"
     gene            40617..42098
                     /gene="tolC"
                     /locus_tag="ACHK07_000239"
     CDS             40617..42098
                     /gene="tolC"
                     /locus_tag="ACHK07_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO8100635.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42246..42917
                     /gene="ygiB"
                     /locus_tag="ACHK07_000240"
     CDS             42246..42917
                     /gene="ygiB"
                     /locus_tag="ACHK07_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO8100636.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            42923..44083
                     /gene="ygiC"
                     /locus_tag="ACHK07_000241"
     CDS             42923..44083
                     /gene="ygiC"
                     /locus_tag="ACHK07_000241"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8100637.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(44121..44909)
                     /gene="ygiD"
                     /locus_tag="ACHK07_000242"
     CDS             complement(44121..44909)
                     /gene="ygiD"
                     /locus_tag="ACHK07_000242"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO8100638.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45052..45825
                     /gene="zupT"
                     /locus_tag="ACHK07_000243"
     CDS             45052..45825
                     /gene="zupT"
                     /locus_tag="ACHK07_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO8100639.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(45883..46053)
                     /gene="yqiD"
                     /locus_tag="ACHK07_000244"
     CDS             complement(45883..46053)
                     /gene="yqiD"
                     /locus_tag="ACHK07_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO8100640.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46315..46968)
                     /gene="ribB"
                     /locus_tag="ACHK07_000245"
     CDS             complement(46315..46968)
                     /gene="ribB"
                     /locus_tag="ACHK07_000245"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO8100641.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47072..47220)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47342..47641
                     /gene="ubiK"
                     /locus_tag="ACHK07_000246"
     CDS             47342..47641
                     /gene="ubiK"
                     /locus_tag="ACHK07_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO8100642.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(47676..47876)
                     /gene="glgS"
                     /locus_tag="ACHK07_000247"
     CDS             complement(47676..47876)
                     /gene="glgS"
                     /locus_tag="ACHK07_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO8100643.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            48145..48774
                     /gene="yqiJ"
                     /locus_tag="ACHK07_000248"
     CDS             48145..48774
                     /gene="yqiJ"
                     /locus_tag="ACHK07_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO8100644.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            48801..50462
                     /gene="yqiK"
                     /locus_tag="ACHK07_000249"
     CDS             48801..50462
                     /gene="yqiK"
                     /locus_tag="ACHK07_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO8100645.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            50701..50760
                     /locus_tag="ACHK07_000250"
     CDS             50701..50760
                     /locus_tag="ACHK07_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8100646.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            51077..51136
                     /gene="ibsE"
                     /locus_tag="ACHK07_000251"
     CDS             51077..51136
                     /gene="ibsE"
                     /locus_tag="ACHK07_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO8100647.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(51256..52689)
                     /gene="hldE"
                     /locus_tag="ACHK07_000252"
     CDS             complement(51256..52689)
                     /gene="hldE"
                     /locus_tag="ACHK07_000252"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO8100648.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQQARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(52737..55577)
                     /gene="glnE"
                     /locus_tag="ACHK07_000253"
     CDS             complement(52737..55577)
                     /gene="glnE"
                     /locus_tag="ACHK07_000253"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO8100649.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPTHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDVMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(55600..56901)
                     /gene="ygiF"
                     /locus_tag="ACHK07_000254"
     CDS             complement(55600..56901)
                     /gene="ygiF"
                     /locus_tag="ACHK07_000254"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO8100650.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAEEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            57143..57763
                     /gene="ygiM"
                     /locus_tag="ACHK07_000255"
     CDS             57143..57763
                     /gene="ygiM"
                     /locus_tag="ACHK07_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO8100651.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            57827..59065
                     /gene="cca"
                     /locus_tag="ACHK07_000256"
     CDS             57827..59065
                     /gene="cca"
                     /locus_tag="ACHK07_000256"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO8100652.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(59246..60067)
                     /gene="bacA"
                     /locus_tag="ACHK07_000257"
     CDS             complement(59246..60067)
                     /gene="bacA"
                     /locus_tag="ACHK07_000257"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8100653.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(60158..60526)
                     /gene="folB"
                     /locus_tag="ACHK07_000258"
     CDS             complement(60158..60526)
                     /gene="folB"
                     /locus_tag="ACHK07_000258"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO8100654.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            60631..61248
                     /gene="plsY"
                     /locus_tag="ACHK07_000259"
     CDS             60631..61248
                     /gene="plsY"
                     /locus_tag="ACHK07_000259"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO8100655.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(61261..62193)
                     /gene="ttdR"
                     /locus_tag="ACHK07_000260"
                     /gene_synonym="dan"
     CDS             complement(61261..62193)
                     /gene="ttdR"
                     /locus_tag="ACHK07_000260"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO8100656.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            62400..63311
                     /gene="ttdA"
                     /locus_tag="ACHK07_000261"
     CDS             62400..63311
                     /gene="ttdA"
                     /locus_tag="ACHK07_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO8100657.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            63308..63913
                     /gene="ttdB"
                     /locus_tag="ACHK07_000262"
     CDS             63308..63913
                     /gene="ttdB"
                     /locus_tag="ACHK07_000262"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO8100658.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            63962..65425
                     /gene="ttdT"
                     /locus_tag="ACHK07_000263"
     CDS             63962..65425
                     /gene="ttdT"
                     /locus_tag="ACHK07_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO8100659.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLMITGLLWMPVVLL"
     gene            complement(65468..66481)
                     /gene="tsaD"
                     /locus_tag="ACHK07_000264"
     CDS             complement(65468..66481)
                     /gene="tsaD"
                     /locus_tag="ACHK07_000264"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO8100660.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            66719..66934
                     /gene="rpsU"
                     /locus_tag="ACHK07_000265"
     CDS             66719..66934
                     /gene="rpsU"
                     /locus_tag="ACHK07_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO8100661.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            67045..68790
                     /gene="dnaG"
                     /locus_tag="ACHK07_000266"
     CDS             67045..68790
                     /gene="dnaG"
                     /locus_tag="ACHK07_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO8100662.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            68985..70826
                     /gene="rpoD"
                     /locus_tag="ACHK07_000267"
     CDS             68985..70826
                     /gene="rpoD"
                     /locus_tag="ACHK07_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO8100663.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(70905..71411)
                     /gene="mug"
                     /locus_tag="ACHK07_000268"
     CDS             complement(70905..71411)
                     /gene="mug"
                     /locus_tag="ACHK07_000268"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO8100664.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            71536..71611
                     /locus_tag="ACHK07_000269"
     tRNA            71536..71611
                     /locus_tag="ACHK07_000269"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71569..71571,aa:Ile,seq:cat)
     gene            complement(71665..72429)
                     /gene="nfeF"
                     /locus_tag="ACHK07_000270"
                     /gene_synonym="yqjH"
     CDS             complement(71665..72429)
                     /gene="nfeF"
                     /locus_tag="ACHK07_000270"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO8100665.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRCFEAEQYDPQRVRAAAYWHAK"
     gene            72717..73340
                     /gene="nfeR"
                     /locus_tag="ACHK07_000271"
                     /gene_synonym="yqjI"
     CDS             72717..73340
                     /gene="nfeR"
                     /locus_tag="ACHK07_000271"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO8100666.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(73447..74967)
                     /gene="aer"
                     /locus_tag="ACHK07_000272"
     CDS             complement(73447..74967)
                     /gene="aer"
                     /locus_tag="ACHK07_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO8100667.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            75385..76764
                     /gene="ygjG"
                     /locus_tag="ACHK07_000273"
     CDS             75385..76764
                     /gene="ygjG"
                     /locus_tag="ACHK07_000273"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO8100668.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(76806..77138)
                     /gene="ygjH"
                     /locus_tag="ACHK07_000274"
     CDS             complement(76806..77138)
                     /gene="ygjH"
                     /locus_tag="ACHK07_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO8100669.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            77357..78340
                     /gene="ebgR"
                     /locus_tag="ACHK07_000275"
     CDS             77357..78340
                     /gene="ebgR"
                     /locus_tag="ACHK07_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO8100670.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            78524..81616
                     /gene="ebgA"
                     /locus_tag="ACHK07_000276"
     CDS             78524..81616
                     /gene="ebgA"
                     /locus_tag="ACHK07_000276"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO8100671.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHVEVRAEGETLTTRQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGKRYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            81613..82062
                     /gene="ebgC"
                     /locus_tag="ACHK07_000277"
     CDS             81613..82062
                     /gene="ebgC"
                     /locus_tag="ACHK07_000277"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO8100672.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            82125..83558
                     /gene="ygjI"
                     /locus_tag="ACHK07_000278"
     CDS             82125..83558
                     /gene="ygjI"
                     /locus_tag="ACHK07_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8100673.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            83692..84762
                     /gene="ygjJ"
                     /locus_tag="ACHK07_000279"
     CDS             83692..84762
                     /gene="ygjJ"
                     /locus_tag="ACHK07_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO8100674.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            84779..87130
                     /gene="ygjK"
                     /locus_tag="ACHK07_000280"
                     /pseudo
     CDS             84779..87130
                     /gene="ygjK"
                     /locus_tag="ACHK07_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            87950..>89963
                     /gene="fadH"
                     /locus_tag="ACHK07_000281"
     CDS             87950..>89963
                     /gene="fadH"
                     /locus_tag="ACHK07_000281"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO8100675.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ANQLQEFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT        22012 a        24199 c        22795 g        20957 t
ORIGIN      
        1 gtactgatta ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca
       61 caattaacgg ttcaatatca ctctcttttt taataaccag tgattcatca ccgcgcaaac
      121 acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca
      181 ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccactgg
      241 ttttgtccag cacctcttca tgatggctga cgaggtcgat gttactgcca cagcgaccgg
      301 cgatcggttt cactgcgtaa cctgttttca ccaattcatc attaacagtg aaatcggtat
      361 ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc
      421 cggggatcac cgtccacagc ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga
      481 taagacgcac ttcattttgc ggatgaccgg tacggatcgg caccgcagca aactcacggt
      541 cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt
      601 taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca
      661 gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg
      721 cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac
      781 tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc
      841 ctttatagcc ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacatgagg
      901 cggagtcggc gttgtactcg taaaccttca gaccacgctc atccatgcag aaatccatac
      961 gaccggtgat catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga
     1021 ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt
     1081 gaagatacat caggtgcagc tcgttggtgg ctttaatcag ctcctgctcg gcgctctcgg
     1141 taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc tgcacatagg
     1201 cgttttgcag cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca
     1261 gacgcgctcc gctgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt
     1321 cagtatcttc cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttaa
     1381 gggtatagca gccgttttcg accaccatct ccagttcgcg cgtccactgt tgcccttgcg
     1441 gcaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca tgtaattggg
     1501 taatgatggc gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg
     1561 cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt
     1621 cattaaccac ttcacgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag
     1681 taaagaccac accgtagttc agaaagagaa aacggcgagc aaattcaacg cattgccatt
     1741 tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct
     1801 gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca
     1861 ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa
     1921 caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta
     1981 accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa
     2041 aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag
     2101 gcaactcact atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc
     2161 cgctggcggc gcgttcgcca atatcaatcg cccggtttct ggtccgacgc atgaaaaaac
     2221 gctgccggtg ggcaaacatc cattacaact ttattcgctg ggaacgccga acggtcagaa
     2281 agtaacgatt atgcttgagg agctgctcgc gctgggcgtt actggcgcag agtacgatgc
     2341 ctggctgata cgcataggcg atggtgatca attctccagc ggctttgtcg aagtgaaccc
     2401 aaattcgaag atcccggcgc tgcgcgatca tacccataat ccgccgatcc gcgtgtttga
     2461 gtcaggatcg attctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggatct
     2521 ggcaaaacgg actgaaacga tgaactggct gttctggtta cagggtgctg cgccgttcct
     2581 cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt acgccatcaa
     2641 tcgctttacc atggaagcca agcgccttct cgacgtgctg gataagcaac tggcgcagca
     2701 taagtttgtt gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg
     2761 caatgtggtg ttaggcggcg tatatgatgc cgcagagttt cttgatgcgg gcagttataa
     2821 gcatgtgcaa cgctgggcaa aagaagtggg cgaacgtccg gcggtgaaac gtgggcgtat
     2881 tgttaaccgc accaacgggc cgctgaacga gcagttgcat gagcgccatg acgccagtga
     2941 ttttgagacg aatacggaag ataagcgtca ggggtaaggg ttagcgttcg tcacagcaag
     3001 ccatccaggc cggataaggc attcacgccg catccggcaa tcgagcacaa tgcctgatgc
     3061 gacgctgccg cgtcttatca gacctacgaa agctaatcat cacgcgctgg taatgtcgta
     3121 atccatttgg cgcagtgcgt ctaatgtggc tttggcttca tcttcgtcga tgatgctcat
     3181 ggcaaatccg acgtgtacca gcacccactg gcccagtaga tcggcagggt taccttcaca
     3241 aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg caagctggtg
     3301 aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg ttaactccgg
     3361 ttattcaact tcaatacttt tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc
     3421 gccatgacag agcggacact gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc
     3481 ccagcaccag gcctgggcgg gtttatagat gatatgtaaa tcgcaccctt gcgccaccgt
     3541 tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc aggagagcgc
     3601 gccaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg cctgccgctg
     3661 gataatttca acggcgctct ggcaaagcga caactcatgc attttcgcca ctcctgcgac
     3721 caaacagcag ggcgcgacgc cctgcgtgtg gtacatccgg attggtgacc ggcagcgaaa
     3781 ggatcattcg tgcgcagtca tccgtcaggc gttgaccctc ttcaatcgac atactgtgcg
     3841 aaagcggtga catcagcgaa caggagaggt attgcgaaac accgtccagt tcgccaacgg
     3901 taaaggtcat ggtgccatac ggcagttgca gaccgatttt ttcactcact ttgcgcagcg
     3961 gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg gtgatcacac
     4021 accccgtcca ctgaccttcg aacagcgtaa aatcagaaac atacaccggc attaaaggat
     4081 gcagaaaaga gagatcgtgc atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt
     4141 gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc ctcccgtggg
     4201 atggcttcca cgccagattc acgcagcgca gccagaacct gctcaagcgc aggttcaatc
     4261 atcgcttcga ccgtcggcgt caagccgatg tgcggctcca gtgattccgg aattacgccg
     4321 accagggtca gctttttcgg aaactcgccg gtgaagcgca gggccgacaa gacgtcggcc
     4381 aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc ttcatcccgc
     4441 aggatcatca tcgttcccgg cgcgctcttc ttcgacacaa tggcatccgc aataatcaga
     4501 tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgtgccgcc atcgaggatc
     4561 tcaacataat ccggcagaat gtatcgttgc tctaacgctt cgacaatccg cacaccgatg
     4621 gcttcatcgg tcagcaaaat attgccgacc cctaagacta aaatacgcat tacagaacct
     4681 tcactgaaac cacttcgttg ccgtcagcat ccactacgtg taccgcacag gccatgcacg
     4741 ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg gcaaccggtg
     4801 tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc ggaccagagt
     4861 tccaggttga tggaacaacc gcctggtagt tgctgatgat accgtcttta ataaccatcc
     4921 agtgagagag cataccgcgc ggcgcttcga ggaagccaac acctttgaac tcacccgttg
     4981 ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg gtgatcagtg
     5041 cactgtattg gttttgcagg atatcctgca attcacagca gtgaacggta cgaccaataa
     5101 tacggcccag cgtggagtgc agctgtgcca cttccagcgt gttgccagtc agtttctgat
     5161 aaatcgcaac gatttcattc agtttgttct gggtagattc gcgacctgcc gccagtttca
     5221 ccagcatatt agccagcgga cccacttcta ccgttttgcc gtagaaagtc ggtgatttca
     5281 cccaggaata tttcccgtcg tcagaccaac catcataagc cggaatggtg gtaccttccc
     5341 acggtgcctg cggcgcttcg tctttatacc aggagtgctt cgcgctttcc tgaatgcctt
     5401 tgatcaggta ttcatcggaa tgagaagtga tcggacgata cgaggacaga tccgcattct
     5461 caatgtagcc gcccgggaac aggaagctgc cgtttttact gtcggtcggg aattccggca
     5521 cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag aacgcagcaa
     5581 taactgcggt atcaacctta taaacctgtt caacaaagtc gctcagcttg tcgatgaaag
     5641 acttgatgta catcaggcgc tcaaggttca gcacgcccag accgtcgagg ttgattgggt
     5701 tagcgacccc acctaccgcc aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca
     5761 cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct accgcgatca
     5821 ggttcacttc cggcggcagt ttcatcgccg ggtgacccca gtagccattg gcgaaaatac
     5881 ccaactgacc gctggcaacc agatctttga tcttgttctg aactttagtg aactcttccg
     5941 gactgttcag atgccaggtc gaaacgcctt tcagcatttc ggaggctttg gttgggtcag
     6001 cttgcagtgc agaagtgatg tccacccagt ccagcgccga aagctgatag aaatgaacga
     6061 tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc gcgttaaccg
     6121 gaacgtcgat attcagcgca ctttctgccg cacgaacgga agacagcgcg tgagtggtag
     6181 tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg cggtttttca
     6241 cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa acgacgccat
     6301 tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa
     6361 ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt ttaaaggagg
     6421 aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc
     6481 aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa
     6541 tgccaccagc gaataggtca gacgccaggt tgcacaacct aacagcgcgc tcagtgccga
     6601 caggaacagc atgcgggaat cattacgcag cttcgccaca cgcagaacca ccagcgggaa
     6661 gagcatcagc aggacttcaa tccagaacat cacggagtag aagtcaccgg caaacgctaa
     6721 cgacagcttg tcgcgataga taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa
     6781 cacactgatg gtgttggtca gcttaacgaa cagactcttt tcatccggac cgttgccacg
     6841 cagacccgcc tgcaccagcg aaccttcaaa gatgacaatc gagaagccca tgatgaacgc
     6901 cgtcagcagc gagaacagcg gcaacatttc atagctctgc cacagcggat gcaccttgta
     6961 gcccgccgag atcatcagcg accccattga agactggtgc atggtcggca gcagcgcacc
     7021 gagcgcgatg atgaagaaca tcaccttgtt cagacgctgt agcgacacct tccagcccag
     7081 acgttcaaac agtgccggag caaactccag agccatcacg ccgatgtaga tggtcataca
     7141 gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt agaagtacgg
     7201 caggttccag tagcgaccca cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa
     7261 cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt taaagacgta
     7321 taccgcccac gccagcgccc agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa
     7381 cgcgatccac acgccccacg ggaagccgcc gttcaggtca gagacagagc ccagaccgaa
     7441 caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa aaatcatgac
     7501 cggtttactg atgattttgc cgcccagcgg ttttggatca tgactcatga tcgtctcctc
     7561 cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac ggttaagccc gccagcacag
     7621 ccagtggtag catcatgcct ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg
     7681 tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc gtcagtacca
     7741 gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat ttcggcaccg
     7801 tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat tcgctgccag
     7861 gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta ccaaaaatca
     7921 ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatcg agacgttcca
     7981 cacctttctg gttgcacagc tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt
     8041 acttcggcac gttgtacgga caggcgacca tgcagtaacg gcagccggtg cacacgtctt
     8101 tgtcgtaatg aacaatgccg gttttcggat cttttttcag tgcagagacc gggcacacag
     8161 agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg tagccgttct
     8221 cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg ttattggtat
     8281 acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga
     8341 agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt
     8401 acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca tgactgacag
     8461 acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa ttacgtctgt
     8521 tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac ccagttcacg
     8581 caccgccatc acactgacac cggcaaccag cccaatcaca ccgccgagca aaccaatagc
     8641 gcctgcagag acgttgccgc cctctttagc gttaacatcc ggtttctgtg aacgcggagt
     8701 ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac cttcttcgtt
     8761 acagccatag cacgggtgac caatcgccac cggccacaca ccgccaacat cgcagaattg
     8821 cagcgttgag cagttgccgt aagtttctgg ccctttacag ccgaggtggt acaggcacca
     8881 gccttcgcgg tggccttcat caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg
     8941 gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac ggtttttgtc
     9001 atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg
     9061 cgggttcggc gggcagcccg gaatgttgat aacggttttg cctggcagaa cttcttgcag
     9121 gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg cagagcagga
     9181 accgatagcg ataatggctg ctgcgccttc cgccgctttg cggatgtgat ccacaatcgg
     9241 ctcaccggca accatgcaat aaataccgtt atcttttaat gggatggaac catccaccac
     9301 taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt cgacctgatg
     9361 accgaaggcg gcggaaagca cttcgtgata ctccagagag atagtctcca gtacgaggtt
     9421 ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc actcctgcgc
     9481 gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca tctctgcagc
     9541 ggctttgcta cttaacccca tggtggcggc taatgctgca caaagcttca tgaaatcacg
     9601 acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata gttattccgt
     9661 tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc
     9721 atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg cgctaaaacg
     9781 aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt tgatcaattt
     9841 tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag
     9901 aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc cgcttcccag
     9961 gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc taatacaaaa
    10021 ccgcgtttat aatcggcaca gaattttgtc acattaaccg cactgtcggc atgcgttgct
    10081 tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct cattcgctat
    10141 atatcgtcat atatagcgat attttaggca aagaacgact tttgcgcacg ctattttttc
    10201 atgcttgttt aagacgtaat cccgtaagag ataaacctat caggagagat atttatcgaa
    10261 ccatttcagt gtcctttgcc aggcaagatc ggcggcagat ttgtcataac ggggcgtgga
    10321 atcattatgg aatccgtgat taacccccgg atagatatac gcctcataaa ccttattatt
    10381 ggctttcaac gccgcctcgt aagcaggcca gccctcgttg attcgggtgt ccagttccgc
    10441 gaagtggagt agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg cctgacgacc
    10501 ataaaacggc accgcgcagg ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc
    10561 accgccatag caaaatccgg taatacccac tttgcctgtc gcttgcggat agcgttgcat
    10621 aaactcaatt gcggcaaaga aatcattcat cagtttggtt ggatcaacct gctgttgcag
    10681 ctcacgacct ttatcatcat ttcccggata acctccaacg gaacttaagc cgtcaggtgc
    10741 cagggcgata taccccgcct tcgccactcg ccgtgccaca tcttcgatat acggattcag
    10801 tccacgattc tcatgcacca ccaccacggc tggcgttttg ccgctcattt ttgcgggttt
    10861 caccagataa ccccgtacct cgccgtgacc atttggcgaa ggatacgtga tgtactcagc
    10921 aacaatctcc gggtcggtaa actctacctg agtcgccagc gcataatttg gcttgagcaa
    10981 atcaaacaac gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa
    11041 ctcacgtttc gagattttcc cgtgagcgta atagtcgtag taatccaaca actcttgtgg
    11101 gaaatctttg gcggttaaac gcggcatcgt tgcactcctc agctggtgtt tttttaagca
    11161 aagcataagc acgtgttttt gcccagtttt tcgtcactct gtgagccaga ctacgggata
    11221 cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag
    11281 gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga
    11341 aaaagcggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt
    11401 aacgcgctgg aatcacagcg tgcgatcctg cgtaaagcgt ttgatttggg cattacgcac
    11461 tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc
    11521 ctgctgcggg aggattttgc cgcttatcgc gatgaactga ttatctctac caaggctggc
    11581 tacgatatgt ggcccggccc ttacggctct ggcggttcac gtaaatacct gctcgccagc
    11641 ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta ctctcatcgc
    11701 gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc
    11761 ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca aaaaatggtc
    11821 gagttgctgc acgagtggaa aattccgctg ttaattcatc aaccttcgta caatttacta
    11881 aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg cgtgggctgc
    11941 attgccttta ctcctctggc tcagggattg ctgaccggaa aatatctcaa cggcattcct
    12001 gaagattcac ggatgcatcg tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctc
    12061 accgaagcca acctcaacag cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa
    12121 tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg agcgagtgac gtcggtattg
    12181 gttggtgcca gccgcgcgga gcaacttgag gagaacgtgc aggcgctgaa taatctgaca
    12241 tttagcaccg aggagctggc gcagatcgat cagcatatcg ccgatggcga gctgaatctg
    12301 tggcaggcgt cttccgataa atgacctgtt aataacgggc gcgagaaccg cgcccgaatc
    12361 agatcagtga ttatgacgag tcagtcggtc aagatagccc attacaaatg cagagagcac
    12421 aaacgtcaga tggataatga cgtaccacat cagtttgtta tcagggacat ttttcgcatc
    12481 cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg ctactttgtt
    12541 tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt tctcggagat
    12601 atcgagttgt gagacgaaat tctcataacc ggaaaacatc accatcacca gcaaaccgcc
    12661 aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt ccgccatcga
    12721 gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca gggcaactaa
    12781 cgcaagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat acattgcatt
    12841 ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca gtatatcgca
    12901 attgagtaaa cagcgcgcaa cagcaaatca acatatccgt taaacctttg tcgcataatt
    12961 ctgagggtta gggatcttcc tggttccctt atcaacgcat cgagagcgtc tacccttaaa
    13021 ggacgttgaa cctgaaggga gaaaaacgat gtctcattta aaagacccga ccacgcagta
    13081 ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg cgaagatgac
    13141 accggtaccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa
    13201 agccctggtg acagggggcg attccggaat cggtcgcgct gccgccatcg cttacgcgcg
    13261 tgaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg ctcaggatgt
    13321 gaaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg atttaagcga
    13381 tgagaaattt gcccgttcgc tggttcacga agcgcataag gccttaggtg ggctggatat
    13441 tatggcgctg gtcgccggga aacaggttgc cattcctgat attgcagacc tcaccagcga
    13501 acagtttcaa aagacctttg ccattaacgt tttcgcactg ttctggctaa cccaggaagc
    13561 gatccacctg ctaccgaaag gtgcaagtat catcaccact tcgtcaatcc aggcatacca
    13621 gccaagtccg catttactgg actatgcggc tacgaaggcg gcgattctga actacagccg
    13681 tggcctggca aaacaggtct cggagaaagg tattcgggtg aatattgtcg cgccaggccc
    13741 gatctggaca gcactgcaaa tttccggcgg acaaacgcag gataagatcc cgcagtttgg
    13801 tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg tatatgttta
    13861 tctggcaagt caggagtcga gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga
    13921 gcatttaggt taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt gtgaattcac
    13981 tcttacctga tgtggcgaaa acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt
    14041 tgcaatagat tttgtaggcc tgataagcgt agcgcatcag gcattgagca cctaatgccg
    14101 gatgcggcgt gaacgcctta tccggcctac aaacgcacga agattcaaca tgttgcaata
    14161 gattttgtag gcctgatgag cgtagcgcat caggcaattc tactttactt cgctttggcg
    14221 gtttcttcac cgaccagacc tatcttcagg taacccgcct gatgcagcgt atccattacc
    14281 ttcatcaacg tctcgtaatc gacggttttg tccgctcgga agaagatggt ggtgtctttc
    14341 ttaccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac cggatcgtta
    14401 ccgataaaca tcgagttgtc tgccttcacc gacagataaa ccggtttttc cggacgcggc
    14461 tgcggcgtgc tggtagaagc aggcaagttc accttcacat ctaccgtcgc taacggtgcc
    14521 gccaccataa agataatcag cagaaccaac atcacgtcga taaacggcgt cacgttgata
    14581 tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcattgccat cggatattat
    14641 cctgcacgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc ttccagatcc
    14701 aggtcacggc tttgcagcaa caatacctgc gctgcgacat cacccagcat cgctttaaag
    14761 ccgccaatct gacgcgcgaa tacgttatag ataacgaccg ccggaatcgc tgccaccaga
    14821 ccgattgccg ttgctaacag agcttctgcg atacccggcg caacgactgc caggttagta
    14881 gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt accaaacagc
    14941 ccaacgaacg gcgaaatcgc gccgatggtt gccagatagc cgttaccgcg gcccatttga
    15001 cgacctactg cggcgacccg acgctccaga cgaaagctgg tacgttcttt aataccttcg
    15061 ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag caaatgcagg
    15121 cttaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa ggaacgcgct
    15181 tcagccagca gttgctgctc gcgcttaagg cgacgcttct gattgaagaa ctctacgctc
    15241 ttactgaaga agattgccca ggtgactacg gaggccaaaa taagcccaat catcacgcac
    15301 ttaacgacaa tatcggcgtg ctgatacata ccccagacgg aaaggtccgt ctgcattaaa
    15361 ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgcgcata ataatatcaa
    15421 aacgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta cctttgcttt
    15481 cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga aaattttctg
    15541 ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag acatccagac
    15601 gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac tggtgaatgc
    15661 aggacgcagc aaaaaataca ctctcggcgc ggtaaatagc gtgattcagc gcgcttcttc
    15721 gctggtattt gagagtatgg aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga
    15781 gttgttctat ggacggcgcg gaacgttaac ccatttttcc ttacaacaag cgatgtgtga
    15841 actggaaggt ggcgcaggct gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc
    15901 gattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg cctatgaacc
    15961 gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat cgtggtttga
    16021 tccgctgatt ggtgccgata tcgttaagca tctgcagcca aacactaaaa tcgtgtttct
    16081 ggaatcgccg ggttccatca ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt
    16141 gcgcagtgtg gtgccggatg ccatcattat gatcgacaac acctgggcag ccggtgtgct
    16201 gtttaaggcg ctggattttg gcatcgatgt ttctattcaa gccgccacca aatatctggt
    16261 tgggcattca gatgcgatga ttggcactgc cgtgtgcaat gcccgttgct gggagcagct
    16321 acgggaaaat gcctatctga tgggccaaat ggtcgatgct gataccgcct atataaccag
    16381 ccgtggcctg cgcacattag gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt
    16441 ggccgaatgg ctggcagaac atccgcaagt tgcgcgagtt aaccaccctg ccctgccagg
    16501 cagtaaagga cacgaattct ggaaacgaga ctttacaggc agcagcgggc tattttcctt
    16561 tgtgcttaag aaaaaactca atgatgaaga gctggcgaac tatctggata acttcagttt
    16621 attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcaa atcaaccaga
    16681 acatatcgcc gccattcgcc cacaaggcga gatcgatttt agcgggacct tgattcgcct
    16741 gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg gttttgcgcg
    16801 gattgtataa cattgccact tttggacaat tttgcagaca ttttattgtg aaaagtctta
    16861 aattgttgcg tccgggatca aggcgtcccg gacgattcag gagtacaata ggcagataaa
    16921 ggcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc atcgctgcgc
    16981 tctggcaaca cgactttgcc gcgctggcgg atcctcatat tgttagcgtt gtttactttg
    17041 tcatgtttgc cacgctgttt ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg
    17101 acagcttgtt gatattggct ggcgcattga ttgcccaggg ggttatggat tttctgccta
    17161 cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat attcaggggc
    17221 gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct gctaaatatc
    17281 atcagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg gctggacgtt
    17341 ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt ctgccaaacc
    17401 gccgtttcca gtttttcaac tggttaagcg gattgctgtg ggtcagcgtg gtaaccagtt
    17461 tcggctatgc cttaagtatg attccgttcg ttaaacgcca tgaagatcag gtaatgacgt
    17521 tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg
    17581 tgattaaaaa aaaatactgt aacgcctgac gattttccct gttcccggtt gctgtaccgg
    17641 gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc cggcgtcaca
    17701 ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc
    17761 gctgctgcgc tggccttcat gccgtcatgg atgatcatca tccgcgcctt atgcagacgg
    17821 taattcttca aatactgcaa cggcgaggtg ctggtgacag acttaaaatt atggtggaac
    17881 gccgatacgc tcatattggc ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat
    17941 ttattctcaa tccgtttcag cacgcggcta atcagactga agtgagtctg gcgactgacc
    18001 agcgccagta acgcgccgcc gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg
    18061 atctgtttgc cgagaatacg cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc
    18121 gccgcgcata aaatctcttc tgataacgtg gcggagttaa tcccgctggc tgccatcgac
    18181 ggctggaaat gctcatcttc gccaatgtcc atcaacagtt cctgtaactg caaaatatcg
    18241 acattgagac gcaaccctgc cagcggcacc tctgacgtgg cataggtttc gcactcaaac
    18301 ggcaacggca ccgtcagcag caggtattca ttggcatcat aacgaaacac gcgttcattg
    18361 atataaccga ttttatgtcc ggaaaagaga attatgatgc caggctcgta catcaccggt
    18421 gtacgtgcga aaggcgtctc gccatacaac aaacgcacat cgggcaacag ttctgacaaa
    18481 ctattttctt tatttttcag tttattaact ttatccgcca gcaagcggca aatctcttca
    18541 cgtttcatat cgcgtaattt cttaggaata atgcggcaat ttgattgtgc gcaattttgt
    18601 agcatttctc cagcactctg gagaaatagg caagacattg gcagaaatga gcattgagag
    18661 ccagggcgct ggcgatcaca atgaaaaaca tcaggcagat cgttctctgc cctcatattg
    18721 gcccagcaaa gggagcaagt aatgaacaac tttaatctgc acaccccaac ccgcattctg
    18781 tttggtaaag gcgcaatcgc tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg
    18841 attacctacg gtggcggcag cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc
    18901 ctgaaaggca tggacgtgct ggaatttggc ggtattgagc caaacccggc ttatgaaacg
    18961 ctgatgaacg ccgtgaaact ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc
    19021 ggcggttctg tactggacgg caccaaattt atcgccgcag cggctaacta tccggaaaat
    19081 atcgatccgt ggcacattct gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg
    19141 ggctgtgtgc tgacgctgcc agcaaccggt tcagaatcca acgccggtgc ggtgatctcc
    19201 cgtaaaacca caggcgataa gcaggcgttc cattcagccc atgttcagcc ggtatttgcc
    19261 gtgctcgatc cggtttatac ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg
    19321 gacgcctttg tacacacggt ggaacagtat gttaccaaac cggttgatgc caaaattcag
    19381 gaccgtttcg cagaaggcat tttgctgacg ctgatcgaag atggtccgaa agccctgaaa
    19441 gagccagaaa actacgatgt gcgcgccaac gtcatgtggg cggcgacgca ggcgctgaac
    19501 ggtttgattg gcgctggcgt accgcaggac tgggcaacgc atatgctggg ccacgaactg
    19561 actgcgatgc acggtctgga tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg
    19621 aatgaaaaac gcgataccaa gcgcgctaag ctgctgcaat atgccgaacg cgtctggaac
    19681 atcactgaag gttccgacga tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc
    19741 tttgagcaat taggcgtgcc gacccacctc tccgactacg gtctggacgg cagctccatc
    19801 ccggctttgc tgaaaaaact ggaagagcac ggcatgaccc aactgggcga aaatcatgac
    19861 attacgctgg atgtcagccg ccgtatatac gaagccgccc gctaagcttt ttacgcctca
    19921 aactttcgtt ttcgggcatt tcgtccagac ttaagttcac aacacctcac cggagcctgc
    19981 tccggtgagt tcatataaag gaggaacgta tggctaatcc aaccgttatt aagctacagg
    20041 atggcaatgt catgccccag ttgggactgg gcgtctggca agcaagtaat gaggaagtaa
    20101 tcaccgccat tcaaaaagcg ttagaagtgg gttatcgctc gattgatacc gccgcggcct
    20161 ataagaacga agaaggtgtc ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac
    20221 tgttcatcac cactaagctg tggaacgacg accacaagcg cccccgcgaa gccctgctcg
    20281 acagcctgaa aaaactacag cttgattata tcgacctcta cttaatgcac tggcccgttc
    20341 ccgctatcga ccattatgtc gaagcatgga aaggcatgat cgaattgcaa aaagagggat
    20401 taatcaaaag catcggcgtg tgcaacttcc agatccatca cctgcaacgc ctgattgatg
    20461 aaactggcgt gacgcctgtg ataaaccaga tcgaacttca tccgctgatg caacaacgcc
    20521 agctacacgc ctggaacgcg acacacaaaa tccagaccga atcctggagc ccattagcgc
    20581 aaggagggaa aggcgttttc gatcagaaag tcattcgcga tctggcagat aaatacggca
    20641 aaaccccggc gcagattgtt atccgctggc atctggatag cggcctggtg gtgatcccga
    20701 aatcggtcac accttcacgt attgccgaaa actttgatgt ctgggatttc cgtctcgaca
    20761 aagacgaact cggcgaaatt gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg
    20821 accagttcgg cggctaacgt gcaaattctc ccggtggcgg taatgttccg ctaccggact
    20881 tttcagaaat catttattcc cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa
    20941 tgccaaatat aaggacatca tcatgcagag ccggaagctc ttaaaagaac aactcatcta
    21001 tatccgggat aaacgcaacg gagaggtgaa aaacagatga aaataatact tctgttttta
    21061 gcagccctgg caagttttac cgtacacgca cagcccccct cacagaccgt agaacaaaca
    21121 gtccggcata tttatcagaa ctataaatca gatgccaccg ccccttattt tggtgaaacc
    21181 ggagagcggg cgataacttc tgtgcgtatt caacaggcgc ttaccctgaa cgacaatctt
    21241 acgctgccgg gtaatattgg ctggctggat tatgatccgg tttgtgattg tcaggatttt
    21301 ggcgatctgg tgctagaaag cgtagcgata accgaaactg acgccgatca tgccgatgcc
    21361 gttgtgcgct ttcgtatctt taaagatgat aaagaaaaga ccacgcagac actgaaaatg
    21421 gtggcggaaa atggtcgttg ggtcattgac gatattgtca gcaatcatgg cagcgtctta
    21481 caagcagtta atagcgagaa tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa
    21541 cagccggaag cctttgttgc cgaactcttt gaacatattg ccgattatag ctggccgtgg
    21601 acgtgggtgg tttccgactc ttaccgccag gcggtcaatg ccttctataa aaccaccttc
    21661 aagacggcca acaatcccga tgaagatatg caaatagaac ggcaatttat ttacgacaat
    21721 ccgatctgtt ttggcgaaga gtcgctattt tcacgcattg atgaaattcg agtcctggag
    21781 aaaaccgccg attccgcccg cattcatatt cgttttacgc tgaccaatgg caacaacgaa
    21841 gagcaagaac tggttttaca acggcgcgac ggtaagtggg aaatcgctga ttttatccgc
    21901 ccgaacagcg gcagcctact taagcagatt gaggcaaaaa ccgccgccag attaaagcaa
    21961 tgagctgaat taaataacaa ttagccggaa caataaaaaa gggaacacta tatgaaaacg
    22021 attttcaccg tgggagctgt tgttctggca acctgcgtgc tcagtggctg cgtcaatgag
    22081 caaaaggtca atcagctggc gagcaatgtg caaacattaa atgccaaaat cgcccggctt
    22141 gagcaggata tgaaagcact acgcccacaa atctatgctg ccaaatccga agctaacaga
    22201 gccaatacgc gtcttgatgc acaggactat tttgattgcc tgcgctgctt gcgtatgtac
    22261 gcagaatgat aaaaaaatcc ccggcagcat gtcagttgcc ggggattttt tttaacgtcc
    22321 aaccgccgct ttagggcgtt tcttcgcacc agcattcacc ggacgagatt gcgtagacga
    22381 cgcttttttt gccgtagcag gcgtctgacg ctgggtcgcc atcggcgtat gtttcgtcaa
    22441 cgctggacga gtattgcggt tctgacgacg agcctcacgc atctcttcaa tggttggcgc
    22501 aggcactaag caatcgcgac ggctgccaat cagatgcttt ttacccattg cttccagcgc
    22561 ctgacggatt aacggccagt ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg
    22621 acgctgtttg tcacccttcg gtacaaagac gtcttcactc ttataaccaa tcttcgccag
    22681 cgggtttttc ccggtgtaat acatggtggt cgagttagcc agcggcgatg ggtagaaatt
    22741 ctgtacctgg tcgagacgga aacgatgctt tttcagccac agcgccaaat tcaccatatc
    22801 ttcatcacgc gtaccggggt gcgcagagat gaaatacggg atcagatact gctctttacc
    22861 tgcctgtttc gagtaagtat cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg
    22921 cttcatcatc ttcgacaacg gcccttcttc ggtatgttcc ggagcaatct tcagataacc
    22981 gccgacgtga tgggtcgcca gttctttgat atagcgcgga tcttccacgg cgatgtcata
    23041 acgtacgcct gaggcaatca gaatcttttt aatgcctttc agatcgcgag cgcggcgata
    23101 gaggttgatc gtcggttcgt ggttagtgtc catatgcgga caaatatccg ggtaaacgca
    23161 ggacaaacga cggcaggttt gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt
    23221 ggcagttggc ccaccgagat cggaaattac gcccgtaaaa cctggaacgg tgtcgcggat
    23281 cgcttcgatc tcattaatga tcgaatcttc ggaacggctc tgaataatgc gcccttcgtg
    23341 ctcggtgata gaacagaaag agcagccgcc aaagcagccg cgcataatgt tgaccgaaaa
    23401 acggatcatt tcgtaagccg gaatacgggc attgccatag gccggatgtg gcacgcgctt
    23461 gtatggcagc gcaaaaacgc tgtccatctc ttcggtagaa agcggaatag caggcgggtt
    23521 gatccacaca tagcggccgc cgtgtttttg catcaatgcg cgggcacagc ctgggttggt
    23581 ttcgtggtgc agaatacgcg aagcatgggc gtacagcact ttatcgccct tcactttctc
    23641 gaaagaaggc agcaacacgt aggttttttc ccacggtttc gggcgcggtg gctgcacggt
    23701 tacggctttg gcttcctgct ttttcggtgc caccggtttg ttatccgcgc acggcaaatc
    23761 ttcgccatac ggatgcggga tcgggtcgat tttcccaggg gtatcaagac gagtagaatc
    23821 cacaccgctc cagcctggca acgcctcttt tacgataatc gcagtattac gcacatcgcg
    23881 gatttcgcta attggctcgc ccatcgccag acggtgcgcc acctccacca gcggacgctc
    23941 accgttacca aacatcagca tgtcggcttt cgaatccacc agcacggaac ggcgcacggt
    24001 atcggaccag taatcataat gcgcggtacg gcgcagacta gcctcaatac caccgaggat
    24061 caccggtaca tctttccacg cctctttaca acgctgggta taaaccagtg tggcgcgatc
    24121 cgggcgctta cccgcgacgt tatctggcgt gtaggcatcg tcatgacgta aacggcgatc
    24181 ggcggtataa cggttgatca tcgaatccat gttgccagca gtaacaccga aaaacagatt
    24241 cggtttaccc agacgcatga aatcgtcttt gctgctccag tctggctgag cgatgatccc
    24301 aacgcgaaag ccctgcgctt ccagcatacg accgcaaatc gccatcccga agcttgggtg
    24361 atcgacatac gcgtcgccag taaccaaaat gatgtcgcag ctatcccagc caagttgatc
    24421 catctcttca cgagacatcg gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg
    24481 cggccatgag aacaggtcgc gatccggttg gatcagggag atagagctca ttttgcttcc
    24541 agaaatgata aaaaaataat caaaggccgg ggattataag ccggaacgaa agagaaatcg
    24601 aaaagtattc catactcgcc ctcctcgggc gagtatgaag attacggtac cggattgact
    24661 aacagttgcc caatcgagcc acggtccgcc atttccagcg tctgactgtt gaagtagaac
    24721 gggaagtgcg cccaggaagg ctgaccgaaa taaacaagca gctccacttg tccgtcaacc
    24781 caaacggtat ctttccagcc tctgtcttcc gggaacggca tcgcaccatt cacgttacgg
    24841 atctggaaca tcacgccttc aatatggaac gcttgcggct cgtccgcgcg taccgtccag
    24901 cgttcccacg ttccttgctg cgcggtgaca tcaatacggt tgacgtccca cagttgtcca
    24961 ttaatacccg ggtcatcacc cagactgata tcgcgactgc gaatcggcga accggccatg
    25021 atttcagttg gcagcaagcg catcggaaga ctgtctgtga ccagcggcag aaggccggtt
    25081 gggcgcagcg ttagcaccag ggtagaaacc agaatgctgg atggctcaaa gaagccacga
    25141 atacgatcaa caatgctcgc cgcttcgcca caggtgatcg acacttcatc accgttgctc
    25201 atatccacca gaatctcgcg gcgctcgccc ggtgccagcg aaagttgctt caccgataca
    25261 ggagcaggca ggaatccctg atcgccagaa atcacatgta acgggcgacc atcgctcatc
    25321 tgtagttgat agcgacgaga gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc
    25381 gagacttcaa cgtacgggct ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg
    25441 ccgcttcccg gttcgttgta ttctggcgta ccaaagttat ccagccgttt atcctgaata
    25501 atgaccggaa aatcatccac accataatgg ttggggatag gcagcgactt gctgacttca
    25561 tcttccacca gccacattcc ggcaaggccg ttatagacct gctgagccgt gcggttggga
    25621 gtattggcgt gataccacag agtagctgcg ttctggcgaa tgggcagtac gggtgcccag
    25681 tcagcgtttg gcgacatcat ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc
    25741 ccggccaccg tcattgagac attttctgtc aggcggttgc tgtaaataag cttaacatcg
    25801 tcgcccttcc agacgcggat agtcggcccc aggtaacgac cattgattcc ccagaccgac
    25861 gcgcgtgtcc ctggcgtaaa tgaccagtgc gcacgttgta cagtcataaa cagcggttgc
    25921 ccacggcgag attcaagcag cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg
    25981 gccttcaggg gaacagcgcc tgcacaaagt gcaatccccg atgcctgaat gaactgacgc
    26041 cgactgagtg acatatttgc tccatgtaaa actggctaaa gtaataccgt gcaggaaagt
    26101 gttccccgca aaaatacaga ttgccgactt aaacttttcc ggcggcttcg cgttctgcga
    26161 cttctttatc gagctcggcg attttttgtt ccattatcga acgacaatgg gcagccagct
    26221 cacgaacctg atctttgcca tactgactga cgtcaattgg cggcagcatt tcgacaatca
    26281 ccagaccgtt gtgcagtcga ttaagattaa tcttattcga agttgtagag acgcacacgg
    26341 gaataatcgg gacgcctgct gcaattgccg cgtgaaatgc tccagtcttg aacggtagca
    26401 ggccgcgacc acgactgcgg gttccttccg ggaacatcca aatggaaata cggcgttttt
    26461 tgaaatgatt cactacttcc gcaatagtgc cgtgagcttt agtgcgattg tttctgtcga
    26521 tcaataagtt gccggttaac cagtacaact gcccgaagaa agggatccac agcaagctct
    26581 ttttacctac cgtcaccgtc ggcggttgca caatgttcga tgctgtcacc atgtcatagt
    26641 tgttctggtg gttagcgata tagatagcat tgccgtagct ttcagcatct gcaggtttgc
    26701 ggcactcaac tttcaggccg aacagcggcg caagacgacc aaacatatgc ccaaaggtgg
    26761 ccacatgttt cgggttacgc gggctgaaaa ggcagtaaat ggagccgaat acacagacta
    26821 agatgctgta aatcacggta ataataagac gaaagatata tagcataaca ccctctgaag
    26881 gtctgagaga ctggcattgt acgtcacctg aaatcaggtt agggcattgt taaaagccgc
    26941 tctctggaaa agcgtattgt taatcgcaac gcatgaataa acaacggttt caagggaatt
    27001 tttatcgttt ccgggggttg aatacgctgc cggatgacga cttaacgttt catccggcgt
    27061 tccttgcaag cgggaggaaa cagcgccctc cccggcatat tactcttcgc tatcaccgct
    27121 gctggcacgg cgaggagagt cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa
    27181 cgtaccgcgg cgtccacgtt cgccagtgac tttctgtaac tcttccgggc gcagtttaat
    27241 tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc
    27301 cagaccatct tctccacgcg cggcttctgc cgatggaatg ttgataatct tgttgccttt
    27361 gcccttcgac agctgcggca gatcgcttac cgggaacatc aacatacggc ctgcctgagt
    27421 gattgccagc agcatatcgg aagcatcttc aattaccacc ggcggcataa catgggcatt
    27481 ttccggtaag gtgatcaaag ccttacctgc acggttacgc gccaccagat cgttaaaggt
    27541 gcagacgaaa ccgtaacccg catcggaagc catcagcagt ttctgatcgt cgctttccat
    27601 cagcatatgg tcaacggtcg ccccaggcgg caacgttaat ttgccggtga gcggttcgcc
    27661 ctgaccacgc gccgacggca gcgtaatcgg gtcaatggca tagctacgac cggtggaatc
    27721 aacaaacact accggttggt tgctcttacc tttcaccgcc gctttgaagc tatcacccgc
    27781 tttataattc aggcccggcg cgtcgatatc atggccttta gcgctgcgta cccagcccat
    27841 ctgcgacagc acaatggtga caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt
    27901 cgcttcttcg cgttcctgca acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc
    27961 ctgcagttct ttcttcagca ggttattcat tttacgctcg gaagccaaaa tgccctgcaa
    28021 ctggtcgcgc tctttttcca gttcactctg ctcaccgcga atcttcatct cttccagttt
    28081 ggcaagatga cgcagtttca gttcgaggat cgcttccgcc tgggtttccg taaggccaaa
    28141 ccgcaacatc agcgccggtt tcggttcatc ttcattacga atgatctcaa tcacttcgtc
    28201 gatattgaga aacgccacca gcaaaccttc gaggatatgc aggcgcttga ggactttctc
    28261 cagacgatag ttcagtcggc ggcgcacggt atcgcggcgg aacaccagcc attcggagag
    28321 aatttccagc aggtttttca ccgccggacg accatccaga ccgatcatat tgaggttaat
    28381 gcgatagctc ttttccagat cggtggtagc gaagaggtgg ttcatcacct gatccatatc
    28441 cacgcggttg gaacgcggca caatcaccag acgggtcggg ttctcgtggt cagattcatc
    28501 gcgcagatcg tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag
    28561 tacgcgcgca cctgaaacct gatgcggcaa tgcgctgata accaccgcgc catcttcttt
    28621 cttccacacc gcacgcatac gcactgaacc acgtccgttc tcgtagattt tacggatctc
    28681 ggcgcgcgaa gtgataattt ccgcttcagt cggataatcc ggcccctgca cgatatccag
    28741 cagctgatcg agcgtggttt tcggctggtc gattaatgcg attgccgcct gagccacttc
    28801 acgcaggtta tgcggtggaa tatcggtcgc catgccgacg gcaataccgg tggtgccgtt
    28861 aagcaaaatg tttggcagac gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa
    28921 gtttggcacc cagtcagccg tcccctgccc caactcgctc aatagcagct cggaatattt
    28981 cgacaaccgg gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc
    29041 ccagttcccc tgaccatcaa ccagcggata acggtaagag aacggctgcg ccatcaggac
    29101 catcgcttca taacaggcgc tatcgccgtg cggatggtat ttacccagta cgtcaccgac
    29161 ggtacgggcc gattttttaa atttggcgct ggcattcagg cccagttcag acatcgcata
    29221 cacaatgcgg cgctgaacag gtttcagacc atcaccaata aacggcaacg cacggtccat
    29281 gatcacgtac atggagtagt ttaagtaggc gttttccgta aattcatgta gcgcaaggcg
    29341 ctctgccata tcgctcatta attctgattc ctcaacttat tcgccagcct tcaaaggggc
    29401 aatattgccg gagatactac attatctgcc gcgtcgagtc acaggagatc agcgtgaata
    29461 gcgccgccgg acgtgctccg acggcgataa aggcatcaat gtgccttgat atacaactct
    29521 ttgctgtaga cataatcctg gggattatgc agcggaaaac cgccaacgta gggtttaagc
    29581 agtttgatta acggctgata gtagatagga atcagcggtg cctgttggtt gatgatcact
    29641 tctgcctgct gatacaacgc attacgcttt gtcgcatcag tgatctgcgt ggcctggttt
    29701 agtaaggcgt catactgcgc atttttccag tgaccgacgt tttcttcact atcgcttttg
    29761 agcgtgttca ggaagctgga agcatcattg tacgtcgcat cccacgactg ccgagacagc
    29821 atgaaatcac cggctcgtcg ggcatcaaga taggttttcc actccattgt gcgcagcgtc
    29881 acctgtgcac ccagccattt tttccattcg gaagacaacg ctatcgcggt cttttcatgc
    29941 agatcgtact tgttgtagaa cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc
    30001 tgtttcagca aggcttttgc catcgcgacg cgctcactca ttggcttttg cagttcatcg
    30061 aacgtcgtcg cgctaaagcc ttttacctct ggcggcgtca gcgtggttgc gggcgttctc
    30121 aaccccagta ccttttgcgc aataagctgt cgatcaaccg taagatatag cgcccgacgc
    30181 acccgcacat cgttaaatgg cggtttctca aggttgaagt tgtaatattc gctgttcaga
    30241 cgcggaataa ttcgtagctc gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc
    30301 ggaacccagg tgagatcgac ctctcccgcg cgatagcggt tatagccggt gaccgaatta
    30361 tccagcgcca gatactcaac ctgttgcaat attgtatgtt gcgcatcgcg gtactttgga
    30421 tttttgcgtg cagtaatctt ttcgttaact acccactgat caagcacaaa ggcaccgttg
    30481 taaaccatgt tctctggctt actccagcta tcgccatgtt tagcgatgac atgatgagga
    30541 accgggaaca gcgtcggcca ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc
    30601 gtaacttcaa gagtacgatc atccgtcgct ttgacaccca gcgatgtaac atctgcttta
    30661 cccgcaacaa tagccgcggc attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg
    30721 cttgccgttt tcgggtcaac cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga
    30781 ggctgaccgt cggaccactg caaaccgcta cgcagatgga aaatatagcg cctgccgccg
    30841 tccagtatct cccagcgttc agcctgagcg ggctgcacct ggccttcacc atccatccat
    30901 accagacctt caaacagatc cagcacaatc tgcgcggcag tattctcctc caccttttgc
    30961 gggtcgagcg tacctgggtc gctatgattg ttgtaacgaa agacttgttg cggagcgagc
    31021 ggtgtgttgg tgggaacgtc agcagcgtag agaggagccg cacttaccag gctgaccagc
    31081 cataataaat ttcgcgtata catggtattc cttaccggag aaattataag cccaacaaca
    31141 atacgcctgt ggcattgttc gctcaaactt atcacgagtg atttggctca cactccggta
    31201 aagattcccc atcacgctaa cggcacataa acatccgttt ccagtcgctg ctctgtcacc
    31261 ccttcggcaa gattggtgta gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt
    31321 gcaggcagcc agtggcgaat aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca
    31381 acgtggcggg ccacggcata acgtccaccg gtaagttcac cattgctcac accataacga
    31441 ttatcgggaa tcggttcgct aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg
    31501 gtggtggcag ggtcgtccca ggcgacgcca aaagtctggc tttggttaac aggagaaagc
    31561 cccgtctctt tacgccacat gataaatttc gccacactgt agttgagcaa ctcagggcta
    31621 cagcggtgcg gcaacatcgc cacagggatt gatgggaaat cgataatgtt tacatccagt
    31681 gtcaggtttg tcattttgcc tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg
    31741 taacaattac ggatttactt tgcggatctg tttgacgtca atttcaacag aattccagtc
    31801 tttatcgact tcaccctgaa tctcaaccgt atctttcggc gtcaccgtca cgccgttcca
    31861 gcgtttgtga tcgatatcaa cattgatagt accgctggca tctttgaaca cgtagagatc
    31921 gtcagagatg cgttcaacga tattgccgcg cagggttacc caggtgtcgt cacgcaggga
    31981 ttttgcgctt tctacagtcg ttacgctgcc gttcggcccc tggaatcctc cggcctgact
    32041 ttgcgttgcc gatgggccag aaaaaccgcc ctgctctgct gccatcaccg gtgcgctgca
    32101 cagggccatt actgcgatta ctgctgcgaa ttttttcatg tttattactc cctttaatgt
    32161 ctgtttccga gcatttaaca agatagtcct taacaacttc ttaagggaaa aaaataaaat
    32221 ttagtgctgt acagagcgcg ttacaacacg gtttactggc agcaaatacg gttatcgcag
    32281 ggatgaaaaa atgcgagttt tactgataga agatgacatg ctgattggcg acggcatcaa
    32341 aacgggcctt agtaaaatgg gttttagcgt cgactggttt acacaaggtc gtcagggaaa
    32401 agaggcgctt tatagcgcac cttatgatgc ggtgatcctg gatttaacct taccaggcat
    32461 ggatggtcgc gatattttgc gcgaatggcg agaaaaaggt cagcgtgagc cggtactgat
    32521 cctgaccgcg cgcgatgcgc tgggggaacg tgtagaaggg ctgcgtctgg gagctgacga
    32581 ttatctgtgt aaaccttttg cgttgataga agtcgccgcc aggctggaag ctctgatgcg
    32641 ccgaaccaac ggccaggcca gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg
    32701 caaacgtatc gccacgctgg ctggcgaacc cttaacactg aaaccaaaag aatttgccct
    32761 gctggaatta ctgatgcgta acgctggtcg ggtactgccg cgcaaactga ttgaagagaa
    32821 actgtatacc tgggacgaag aggtcaccag taatgccgtt gaagtgcatg tgcatcatct
    32881 gcgacgcaaa ctcggtagtg attttattcg taccgtgcat ggtattggct acacattagg
    32941 tgagaaatga aatttaccca acgtcttagt ctgcgcgtca ggctgacgct aatcttttta
    33001 attctggcct cggtgacctg gctgctttcc agctttgtcg cctggaaaca aacaacggat
    33061 aacgtcgatg aattgttcga cacccaactg atgctttttg ccaagcggtt aagtacgctc
    33121 gatctcaacg aaatcaacgc ggcggatcgc atggcacaga cgccaaataa attaaaacac
    33181 ggtcatgttg atgacgatgc gctgaccttt gccatcttta cccacgacgg cagaatggtc
    33241 cttaacgatg gcgataacgg agaagatatt ccctatagct atcaacggga aggttttgct
    33301 gacgggcaac tggtcggtga agacgatccc tggcgttttg tctggatgac ctcacctgat
    33361 ggcaaatatc gcatcgttgt tggtcaggaa tgggaatacc gtgaagacat ggcgctggcg
    33421 attgttgccg ggcaattgat cccgtggctg gtcgcactgc cgattatgtt aatcatcatg
    33481 atggtactac tgggtcgtga actcgcgccg ctgaacaagc tggcgctggc gctacgtatg
    33541 cgtgaccctg actcggaaaa accactaaac gcgactggcg tacccagcga agtgcgacca
    33601 ctggttgagt cgctaaatca actgttcgtc cgcacacatg cgatgatggt tcgtgaacga
    33661 cgctttacct ccgacgcagc tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa
    33721 accgaagttg cgcagctctc tgacgatgat ccgcaggcgc gaaaaaaagc actgcttcaa
    33781 ttacattacg ggatcgatcg cgctactcgt ctggttgatc aactgctcac gctatcgcgg
    33841 ctggactcac tggataacct tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa
    33901 tcgtcggtga tggatattta ccacacggcg cagcaggcga aaattgacgt gcgactgaca
    33961 ctcaatgtcc agggcatcaa acgcaccgga caaccgctat tgctaagttt gttggtgcga
    34021 aatttgctgg ataacgccgt gcgttacagt ccacagggca gcgtggtgga cgtcacgctg
    34081 aatgccgata atttcatcgt gagggataac ggccccggtg tgacaccaga ggcactggcg
    34141 cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg ctaccggcag tggacttggg
    34201 ctatcgattg tccagcgaat cgccaagctg catggcatga atgttgagtt tgggaatgcg
    34261 gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc tctgtctatg agagccgtta
    34321 aaacggctct catagatttt actaatagca aaatataaac cgtccccaaa aaagccacca
    34381 accacaaacc agacaaataa cgaacgagca tacatataac caaaactcaa acatttcccg
    34441 cacaaagagt tttcttaaga taataataat aagtggcgtc agaagaaaaa atgctgcaat
    34501 cattgcttgc aatatacgat cttcgcctga gacggaaggt atactgcaaa tattgtctcc
    34561 cgagtcaagc atccagtcat acttattgaa agcaaaaaaa gcatctatta accaataagt
    34621 aagcattata tataacagag cttcaaatat tgtttgaatt tcatgcttta acatatgtta
    34681 gcactctcta aaaaagtcca gtcggagatc tggttttcga taccctccag taaatctatt
    34741 taaaataatc tgcgtaacac gctgatattt tcatttttag ttattcaaca ccacaattat
    34801 caactcgcag aaagtccaca ccaacgttgt aaattaaccc tgtgctagtt ggaatgatga
    34861 gaaattgcta tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag
    34921 caagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcaccgta
    34981 ccgctctgtg acgtactata gtcggcaaac gtctcacctt gaggttaaaa aatgagcaac
    35041 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
    35101 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgttcg catcgttcgc
    35161 gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
    35221 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atacattgat
    35281 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
    35341 ccgtcgaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat gctttctctg
    35401 acctggaacg cgccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggc
    35461 gttgacggtg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat ggaaccgctg
    35521 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
    35581 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
    35641 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
    35701 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
    35761 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
    35821 atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct gcaaaccccg
    35881 cacatgaagg cgtatagcga agccgtaaaa ggtgacgtgc tggagatgaa tattcgtatt
    35941 ctgcaaccgg ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct
    36001 ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccaatt gcggcgatct
    36061 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
    36121 tcatcgatag tcagctgcac cagacggcga gtgttcggat caagcgtggt ttcgcgcaat
    36181 tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg
    36241 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
    36301 ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc
    36361 aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca
    36421 gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag gtcgtcgctg
    36481 tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg
    36541 tcggaagaaa cttcccaggt gttaaggatc ttacctttca gcggcatgat cgcctgatat
    36601 tcacgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac aaggaacagt
    36661 tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag cgccgggccg
    36721 ctggtcagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
    36781 atcgccatct ctgccagcaa ctccgccgcc tgaacgttct ggttcagcca caggatgaag
    36841 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgctctttg
    36901 gtctgcccgg caaactgcgg atcctgcatt tttactgaca gcacataggc gcagcgatcc
    36961 cagatatctt ccgccgacaa ctttacaccg cgcggcaaaa tgttgcggta ttcacagaac
    37021 tcacgcatcg cgtccagcag gccctgacgc aggccattaa catgggtacc gccctgcatc
    37081 gttgggatca ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
    37141 agcgcccagt ccaccgcttc agtatcgcca gcgaaattac cgataaaggg tttttctggc
    37201 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
    37261 cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt
    37321 actgctttgg ctttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctatcgaag
    37381 aaggtttcat ccggccagaa atggacgctg gtgccggtat tgcgtttacc gcaagtgccg
    37441 acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca
    37501 tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga aatccccacg
    37561 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga
    37621 cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc caccggcatc
    37681 ccgcgcccat cgtcaataac ttctaacgac tggtcagcat gtaaaataac gtccacgcgt
    37741 tttgcgtgac ccgccagtgc ttcatccaca ctgttatcaa tgacttcttg ccccaaatgg
    37801 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
    37861 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
    37921 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
    37981 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc
    38041 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
    38101 cggcgcttcc agcgggtcaa tctgcatgac tttaagatcg taaatatggc gtgactctag
    38161 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcagttc
    38221 aaacgggcgc accgccgggt ttaccaccac tgcgggcagc ataaaacatt gtgacaacca
    38281 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
    38341 caggacaatg gattccagca gctctgccgc gtcggaagga tacggcggca actgcggaat
    38401 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
    38461 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
    38521 gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg
    38581 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tagtatccag cgtaaagttg
    38641 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc
    38701 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
    38761 ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc aagccaacta
    38821 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgctc tggcgcatcg
    38881 gccagtttac gttccagcca ctcaagctga aactcgctta gctcaccgtg cggcacgcca
    38941 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac gcgcttcgcc
    39001 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc
    39061 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
    39121 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
    39181 tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
    39241 gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct gactctggcc
    39301 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
    39361 tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
    39421 aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
    39481 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
    39541 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
    39601 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
    39661 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
    39721 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
    39781 atttataccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
    39841 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
    39901 accacatgaa cgcgaatatc ttcgttttca tcagccagac cgtgaatacc gcttgcggtc
    39961 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
    40021 aggaaactta acaccggttt ggtccgtttg actatcagcc ccgcctcttc aatcgcttcg
    40081 cgacgggcaa catcttccac actttcaccc tcttctatca tcccggcaac catctccagt
    40141 agccaggggg tttcgctagt gtcgtacgcg gcaattcgaa tctgctcaat cagcacaact
    40201 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
    40261 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
    40321 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
    40381 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat
    40441 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc
    40501 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
    40561 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
    40621 agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg
    40681 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
    40741 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
    40801 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
    40861 tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
    40921 aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga
    40981 ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
    41041 ttgacgttct ttcctataca caggcgcaaa aagaagcgat ctaccgtcaa ttagatcaaa
    41101 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc
    41161 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
    41221 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
    41281 actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca
    41341 acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa attcgccagg
    41401 cgcaggatgg tcacttaccg actctggatt taacggcttc taccgggatt tctgacacct
    41461 cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
    41521 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
    41581 cgcaggtgaa acaggcccag tacaactttg ttggtgccag cgagcaactg gaaagcgcgc
    41641 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
    41701 gtattaacgc ctacaaacaa gccgtggttt ccgcccaaag ctcattagac gcgatggaag
    41761 cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
    41821 acaacgccaa gcaagagctg gcgaatgcgc gttataacta cctgattaat cagctgaata
    41881 ttaagtcagc cctgggtaca ttgaacgagc aggatctgct ggcactgaac aatgcgctga
    41941 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
    42001 ttgctgatgg ttatgcgcct gatagccctg cacccgtcgt tcagcaaaca tccgcacgca
    42061 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
    42121 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
    42181 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
    42241 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
    42301 gccatctgac accagtcgct ctcgcagttg ccactgtttt tatgctggct ggctgtgaaa
    42361 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
    42421 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
    42481 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc
    42541 aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag caatccagcg
    42601 ggagtttctg gatgccgctg atggccggtt acatgatggg tcgtctgatg ggcggcggcg
    42661 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagcccg gcttacggta
    42721 aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
    42781 taccgaagac agccatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt
    42841 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggt acctcttctc
    42901 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
    42961 tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
    43021 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
    43081 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
    43141 gaccaaattc cgcattccaa aacacacctg gagttttgtg cgccagtcat ggctgacgca
    43201 ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact
    43261 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
    43321 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
    43381 tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
    43441 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggtaccattc agtatttgca
    43501 ggactgcgcg acggaagctg aaatcgctac cgagttcctc tacattgacg atatcgggtt
    43561 aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc tgttcaagct
    43621 gtatccgtgg gaatttatgt tgcgtgagat gttttccacc aagctggagg atgcaggcgt
    43681 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact
    43741 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    43801 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    43861 gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat
    43921 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    43981 ctggctggtg aacgatcaac ccgccggaat tggcattcgt gaagaccgtg cattgatcac
    44041 ccaggatatg tctcggtttt atccacatat ttttgttgaa taatcaatga tgccggatgg
    44101 cattcgccat ccggtaattg ttagcctatc tgcaccgaca gcatacttaa actgcccatt
    44161 tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc taacacatac
    44221 aacaacggca gatagtgctc tggcgttggg ttcgataacg cgccaccttc atggtcgaga
    44281 taattcacca gaggatgctg ctctactggc ccttgccacg tcagattcgc tttcacatac
    44341 tcattaaacg acgtcgccca cggatacggt gaactatcac cgtgccactt cactgtacgc
    44401 aggttatgca ccacgttgcc gctggcgacc aacattattc cttcatcacg cagcgccgcg
    44461 agttttcgcc ccatttcgaa atgccaggcg gcaggtttgc tactatcgat actcaactgc
    44521 accatcggga tatcggcgtc aggatacatc ttaatcagca cgccccagga accgtggtca
    44581 aagccccagg cttctttatc cagcgtcacc gggaccggcg ctaacagctc aaccagatgc
    44641 tgtgccagcg caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag
    44701 ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta
    44761 aaccagtgag ccgaaactac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacgtc
    44821 tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa
    44881 aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg
    44941 atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca
    45001 tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta
    45061 cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt
    45121 ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc ggcggggatc
    45181 atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg
    45241 cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg cctggaccgc
    45301 atgttgccac atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa
    45361 tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa
    45421 gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgctctg
    45481 gccgtagcgt tgcacaatat tcctgaaggt ctggcagtgg caggcccggt ttacgcagca
    45541 acggggtcta aacgcacggc gattctgtgg gcggggattt ctggactagc agaaattctt
    45601 ggtggcgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt catggcagca
    45661 atcatggcgg cggtcgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc
    45721 gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg
    45781 atgggattca gtttggtgct gctacaaacg gcgggaattg gttaaaaaca acatcagaag
    45841 ttgtccagcg catggacaac ttctgctagt ttcactaaag ccttattcta ccttttcggc
    45901 tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc
    45961 ctggcgaaat gcccgacaat aacgtttcaa atgcaggaaa taacccacca caaccagaac
    46021 aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat ttaacaatac
    46081 taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata tggtcgcata
    46141 ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat attaatgaaa
    46201 ttttctcata gaaagagaag cggaattaac atcttgcata gcagggaaaa ataaaaaccg
    46261 aagcctgcta caacttcggt ttcttgatta aggcagtaaa ttaagcagcg gttttcagct
    46321 ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca caagcgccat
    46381 attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt
    46441 agtcagctca cacagcacac cagccggttt gaagcctgcc agcgtcatta gatcaatagt
    46501 tgcttcagta tgaccgccac gcgtcagtac accaccagcc tgagcgcgga gtgggaaaac
    46561 gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt
    46621 cgtaatacgg tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac
    46681 ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca gatcgagttg
    46741 tttacggcga tcttcagtaa tgcacaggca aacaatacca ctaccgtggc gaatggtcag
    46801 cgccatctgc tcaacagtca tggtttctgc cgggaagatc atatcacctt cgttttcacg
    46861 gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc
    46921 aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc
    46981 tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa
    47041 ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc
    47101 gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg
    47161 ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc
    47221 ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt
    47281 tatgacttac ccttatcgca ctacaatggc actcaacacc tatcagtaca gggaaactac
    47341 aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa
    47401 aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagcgca
    47461 gctgacgcgt ctcgatctgg taagccgcga agaattcgac gttcagacgc aagtcctgtt
    47521 gcggactcgt gaaaaactgg cgttactgga gcagcgcatc agtgaactgg aagcgcgcaa
    47581 taattcggta gcagatcttc aatctcctcc ggctatcccg cctattgata aagcagaata
    47641 aagcaacaca aaaacgggcc agacggcccg taatatcagt gttctaattc aagttctctg
    47701 gtttgcatca tctgttggca ataccaggca taacgtgcgc aaaaccaggt tctatgttct
    47761 tcatccatgt taccagtaac ggccagaata tcgaccgggc gaccttctgc gtgcattctg
    47821 gcaaaactac gcgccaggaa atcgaaatta tttaaagaat taagactatg atccataagc
    47881 acatcctccg gttatcgcat accttttaag gccatatgct ttttctgatt tcagtattcc
    47941 ccaatcagtg tagtgccttt aaacataacg tgcgtaaata tattgtcccc gatcacactt
    48001 tttagtgaaa aataaaaaac catattaaaa gactttctct tccagaactt ctctaacatg
    48061 tgttattatt aacaaagaca aatgtaagca aggaaactat cccaaagtaa atattgcgtc
    48121 actaaatgga cattggagtg acatatgatt ttattcgccg actataacac cccttatctt
    48181 ttcgctattt cctttgtact gttgatcggc cttttagaaa tttttgcgct tatctgcggt
    48241 cacatgctct ccggtgctct tgatgcacat cttgaccatt atgattccat aaccacaggt
    48301 catattagcc aggcacttca ttatctcaat atcggaagat tgccagctct cgtcgttctc
    48361 tgcttgctgg ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc ctgcatcatg
    48421 gtctggcaat caccactgtc gaacctgttc gtggttcctg tcagtttact gtttacgatt
    48481 attgcggtgc attataccgg taaaatagtt gcgccctgga ttccacgcga ccatagttca
    48541 gctattacag aagaagaata tattggtagc atggcattaa ttaccggtca tcaagccact
    48601 tcaggtaatc cctgcgaagg aaaactcacc gatcaatttg gccaaataca ttatttacta
    48661 ctggagcctg aagaaggaaa aattttcact aaaggagaca aagtgttaat tatttgccga
    48721 ctttctgcaa cgcgatattt ggcggaaaac aatccctggc cccaaatatt gtaatttatt
    48781 aatcaaaagg aaatttaata atggatgata ttgttaattc tgtgccctcc tggatgttta
    48841 ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc aggctctatc
    48901 gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa aaagtggtaa
    48961 tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc aatatgaata
    49021 ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa gatcgtatgc
    49081 gcgtcgatgt agtagtcgct ttctttgtgc gggtaaaacc ttcagtagaa gggattgcca
    49141 ccgctgccca gacgctgggg caacgcaccc tgtcgcctga agacttacgt atgttggttg
    49201 aagataaatt tgtcgatgcc ctccgtgcaa cagctgcgca aatgaccatg catgagttac
    49261 aggatacccg cgagaacttc gtgcagggag tacaaaatac cgtagctgaa gatctgtcga
    49321 aaaacggcct ggaactggaa agcgtttcac ttaccaactt taaccagacc tcgaaagaac
    49381 atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgact caggagacgg
    49441 agcgccgtcg ccgcgagcgt aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga
    49501 aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg tttatgacgc
    49561 ttgagcagga gcagcaggtt aaaacccgta ccgctgagca gaatgcgaaa attgcggctt
    49621 ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa cgacagattc
    49681 aggaaacaga aatcgaccgc gaacaggccg tccgctcaag aaaagttgaa gctgaacgtg
    49741 aagttcgcat taaagagatc gaacagcagc aggtcaccga aatcgctaac cagacgaaat
    49801 cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt gctaaccttg
    49861 cactcgcaga agcagtaagc gcccaacaaa acgttgaaac cactcgccag accgcagaag
    49921 ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa accaaagcgg
    49981 ttgaactgac cgtgcgggca aaagcagaaa aagaagccgc agagatgcag gcggcggcta
    50041 tcgttgagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg
    50101 cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa ttcaaactgg
    50161 ccttgttgca ggcgctgcct gcggtaatag aaaaatccgt tgagccgatg aaatccattg
    50221 acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg ggtgatgcaa
    50281 acacgggtaa tgtggggggc ggaaacctgg cggaacaagc gttatcagcc gcactctctt
    50341 accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt tcaggcggct
    50401 cactggcggc attgacttca cccttaacct caacaactcc cgtcgcagaa aacgtagaat
    50461 aaattttcaa ctatgaacat ccctggccca aaaagccggg gattttttat atctgcgttc
    50521 cgctaaaaac tgcaaatgat caggttgttg caacgttttg cgcgaccgct cggggaaggc
    50581 aaaactgcca cagggaaagc attgcgcgag gtccggcgct catcaacaat cgggaagtca
    50641 gcaaggggct gaaacgggaa agcccctccc gaagaagggg ccttacagaa ggaaagggtt
    50701 atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc agcttattaa
    50761 cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt taggttgaaa
    50821 aaaaatcagc gtcaagaagg tttcattaac acatatccct ggcccaaaaa gccggggatt
    50881 ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg
    50941 accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc
    51001 aacaatcggg gagacagcaa ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt
    51061 atagaaggaa agggttatga tgaagctcgt catcatactg gttgtgttgt tactgttaag
    51121 tttcccgact tactaacaac tcatcagagg ggggagaaat cctcccttac ccttgttcct
    51181 ttactctagg ttgaaaaaac aacagcgtca ataggcctgc catgtacgaa gcgagatctg
    51241 tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgataa tgttagtggt
    51301 cgagcaaccg tcttcaaagt tgagcaccaa cacttcgccg ccgttggccc agacttcttt
    51361 actcccggcg atctcttccg gtttgtagtc accgcctttc accagcaggt ctggcaggat
    51421 cccggcgatc agacgctgcg gcgtgtcttc ttcaaacgac actacccagt cgaccgcttc
    51481 cagtgcgccc agcacaatca tacgctgttc gagtgggttt accgggcggg aatccccttt
    51541 cagccgtttg gtggaggcat cgctgttgac ggcaacaatc aagcggtcac ccagcttgcg
    51601 ggcatttgcc agataagaga cgtgcccggc atgtaggata tcgaagacac cgttggtcat
    51661 taccactttt tcaccacgtt tacgcgctgc cgctacagcc agcttcagtt cctcttcggt
    51721 catcacgcca aagccggtat ctgcgcgtcc acgtacagcg ttttccagct cgatcggcga
    51781 aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg caaagaagca
    51841 ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc
    51901 gcccgcaccg gtaacgtcat acacttcctg cgcttgggtt ggcatatgca gcggcgcttt
    51961 acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag ccgagagttc
    52021 gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct tacatttacc
    52081 gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc ggtagcgctc
    52141 aaaatcggta ccttttggat caatcagcac cggaacaccc gctttacgcg cctgttggat
    52201 catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca gcgcgccaat
    52261 cgaactcagc gcctgattaa tccgttcgtg cagcggttgc ggatcaacac cttcgaaacc
    52321 ttcttcaaaa tccaggcgga tcagctgttg gttgcgggaa agtacccgta atttggtgat
    52381 ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg ccagagattt
    52441 actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg cattagcacc
    52501 gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt cgatggtatt
    52561 cactttaacc acgggcaccg gcgcttccgg cgagatacga ctggtggggc cgtaccagta
    52621 acgatccagc atcacatcac caaccaccat cactcctgca cgttcaaact ctggcagcgt
    52681 tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac catacttcat
    52741 tcttccacca gccacttctg ccagcttgcc cgcaccaaat cacgctctgc ggtgaagcaa
    52801 tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc atcgcgcagc
    52861 gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc gttttgcgcc
    52921 agcaattcca gaatgcgcac gttgtctgac cagcgcgtta acttcggttt ttcatgagcg
    52981 tagcgcaaca ccagatattg agtaataaat tcgatatcgg taattccgcc ttcatcggct
    53041 ttgatatcaa agcgatcgcg atgtttattg ccgagatgag cgcgcatttt ttcgcgcatt
    53101 tcccgcactt ccgtttgcag agttttacct tcacgcggca gcgtcataat ctcgcggcgc
    53161 accgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc acgcaccagc
    53221 gcctgatgtt cccacgtcca ggcctcgttt ttctgataat cagcaaatgc ttctgcggat
    53281 gtcaccagca ttcccgccgc cccggacggg cgtagccgtg catccacttc atacaaaatg
    53341 ccggaagaag tacgcgtgct gaacagatgc ataatgcgtt gcgccagacg cagataaaac
    53401 tgccgcccgt cgatttcccg ctcgccgtcc gtcattacat ccatcgggca atcatggaga
    53461 aagatcaggt caagatcaga actgtagcct aactcccagc cgcccagctt gccgtagccg
    53521 accaccgcaa agccgcgacc ttcacgttca ttcaggtgag tcggttttcc gtaacgggca
    53581 accatttgaa cccacgcttg ctggaccacc gcatctatca ttgcctccgc cagccaggtt
    53641 aagtgatcgc tcactttcat taccggcagc gtaccggcga tatccgctgc cgcaatgcgt
    53701 aacagttgcg cctgtttgaa ctgacgcagc gcctcaagct gttgttcttc gtcatcttcc
    53761 ggcacgcgca gcaaatactg gcgcaactca tcacggtaag catcggtcgc cgtcggctgg
    53821 tagagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc cagctggctg
    53881 gcaatcatcg gcgacgcggc acacagagaa atcaaatgtt taagcgccgc ggggaattca
    53941 ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc ggtaatgcgc
    54001 gacagcgtga cggcagcgtc ttcacgcgca cagacatcac ttagcagatg tggcatcaga
    54061 tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct tatccagctc tttgcggaaa
    54121 tcagcaatca gcgttagcac ctgtttgcga tcattttcgc taagatgcgc cagcactggc
    54181 gtggtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc cgacagcgac
    54241 tcttcctgag tttcactttc atcgtcgcca atcaattcat taaacacccg gcgcacattg
    54301 gtcatatgtg cggtcagcgc cacggtcagt tgcggccagt cagcaaagtc catcgcccac
    54361 gccagccgcg cacggttaag ctcatcagaa ggaagcgtct gggtttgttc gtcgttaatg
    54421 ctttgcagca ggttttccag acgccgcagg aacagatacg ccactcgcaa ttgttcagca
    54481 tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag taaagagcgc
    54541 gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac gataaattca
    54601 atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg tcgacgcact
    54661 tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa
    54721 cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac gccttcgcta
    54781 tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg
    54841 taataatctt ccagcgccgc aaagctcagc accagcgggc cactttcgcc aaacggacgc
    54901 agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccag cactttaatc
    54961 agccgctgcc ccatgcgggt aaaaaactgg gcgttatcca gttcccggcg tccaccctgc
    55021 gtacaaccat gttccggcca ggcaaaaatc agatcgatat cagaggagaa attcagttcc
    55081 ccgccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc ctgcgcattg
    55141 cacggcgttc cccactcgcg gcagcaggcg tcatatagcc agtcacgcgc cgcaacaatc
    55201 agcgtctccg ccagatagct gagctgctgc aatatgctct cttcagtaac cagtgccagc
    55261 gtttgcgccc aggcgatacg taccatgatg cgccgacgga acagccgcag ctcgcgcatt
    55321 aacccggctt cgtcactcac attactgagc gcctcctgca accatgcaac gtaatgctgc
    55381 cattcgtcgg cctgcggcgg ttggctttcc agttccgtca gccactctgg atgcgcaatc
    55441 acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc gctaagtgat
    55501 tcctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg ctgtaacggt
    55561 gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc cgctgtgcaa
    55621 ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcga tgcgttgccc
    55681 ggtcgcaata gcgtgatgca gcccctgcca gttctccagc caggcttgcg cgacgacagg
    55741 atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcaggc gtggcaactg
    55801 gtcacggtag cgatcgccta acggctggca gaaaacgctt ttcagttcag cggcatggcg
    55861 ggaaagatgg atatcggcaa agcgtttgaa ggagtcgctg attttgccct gcgctttggc
    55921 atctaaaaat ggctgccatg ctttgcttac caaccattcg gtcaacgcca gcttcgccat
    55981 tgccgtttcg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcaa tggtcgcttc
    56041 gcattgggtc agcaaatcac gtaagtgagt gctcgcttta cgcggcacaa taccaccgaa
    56101 cagcatcagc gtatgacgga ccaggctaat ggctgccagc acctgttctt tcgccgcatc
    56161 gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca gctcgagcgc
    56221 cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg tggtcggttt
    56281 gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt tgctcaggct
    56341 gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca ccgcgcgcgt
    56401 gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt cctccgcttt
    56461 cacctccccc tgatcgagag cgatttcaat tcggctgcca tcgaccgcca ccagccattt
    56521 ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat cggcaggcaa
    56581 ttcgccgttc ggccagactt ccgagggaaa ctgcgccagg tcgagcgtcg gttcgctcaa
    56641 cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg caactttcat
    56701 ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt gcccacgcag
    56761 ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat ggtgctcgcc
    56821 gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa caataaactt
    56881 taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa gtgacgatga
    56941 acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca aattgccatg
    57001 caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga cacagaggat
    57061 tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc gttccacttt
    57121 ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa ctttactcgc
    57181 acttagcgcg actgccgtct cacacgctga agaaacgcgc tatgtttctg acgaactgaa
    57241 tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg
    57301 cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg tgaaagacag
    57361 ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa gcctgcgctc
    57421 ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca ccaatatcga
    57481 taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga gcgacagcgt
    57541 gatcaacggg ttaaaagaag aaaatcagaa actgaaaaac gagctgattg tcgcgcagaa
    57601 aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca tcatgcaatg
    57661 gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg tactgccgca
    57721 cctgatccca agccgcaaac gcaaagatcg ctggatgaac taaatcgcct tcttcgttgc
    57781 actgtcttat tattaggaat tgtgtattta acgaagggat gatggcgtga agatttatct
    57841 ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca gagattgggt
    57901 ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg taggccgcga
    57961 ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac gtaccgaacg
    58021 gaaatccggt tccggttaca ccggttttac ctgctatgcc gcaccggatg tcacgctgga
    58081 agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg ataacggcga
    58141 gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc
    58201 ccccgctttt ggcgaagatc cgttacgcgt actgcgcgtg gcgcgttttg ctgcgcgtta
    58261 tgcccacctc ggttttcgta ttgccgatga aactctggcg ttgatgcgcg agatgaccca
    58321 tgcgggtgaa ctggaacacc tgacgcctga acgggtgtgg aaagagacgg aaagcgccct
    58381 caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg cacttcgcgt
    58441 tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt ggcatccgga
    58501 aatcgacacg ggtattcata ccttaatgac gctctcaatg gcggcgatgc tgagtccgca
    58561 ggtcgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga cgccgccaga
    58621 actctggccg cgtcatcatg gtcatggccc ggcgggtgtt aagttagtgg aacaactatg
    58681 ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg ctgagtttca
    58741 cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat tatttgattc
    58801 catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga
    58861 cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt ggttgcgcga
    58921 agcctgggaa gtggcgcagt cagtgccgac aaaagccgtc gttgaagcgg gatttaaagg
    58981 cgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca gctggaagga
    59041 acaacgttgc ccaaagcctg aatgagtatt gggcattttg ccggatgcgg cgtaaacgcc
    59101 ttatccggct acatgtcaac gacagttgta ggcctgataa gacgcgcaag cgtcgcatca
    59161 ggcgttgatt gccggatgcg gcgtgaacgc cttattcggc ctacgcaatc gtggacaacg
    59221 ccaggcatcc aacactattc ctcaatcaaa agaacacgac atacaccgca gccgccacaa
    59281 taaagcgata aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt
    59341 taatcgctat cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat
    59401 cgccggttgt caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca
    59461 tcggcaccgc cagcaggaac gaaaactcgg aagcagcgta acggctcacc cccatcagca
    59521 tcccacctga aatggtcgcc ccggaacggg agaaacccgg ccacagcgcc agacactgga
    59581 aacagccaat cataaatgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct
    59641 ctttcggctt caggcattcg gcggcaatca gcaacaaacc gccaacgacc agcgcataca
    59701 tcacatttat cgggttaaac aatgacttaa tcgtgtcgtg gaacaacagc cccagtacca
    59761 ccgccggaat catccccagc aaaatgtgga tcagcgttaa acgacctttg ctttctcctt
    59821 cgtgctgcaa cgggcggcca aagtggatgc caatcaggcc aaacagacgc cgccagaaca
    59881 tcactactac cgccagaatt gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt
    59941 cgccctcaaa ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca
    60001 gaaattctgt caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat
    60061 cgctcatcaa taaaccccta aattattaaa atgtaccgct tgtccgaact actgcagtat
    60121 gaccaggtta taaccgtttg gtttaacagc tgtaaaatta attattttct ttcagattat
    60181 tgccacgctc aatgattacg ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga
    60241 gtttgatacg cacccacggc gagttgaagc gtgctaacag cagctccgcc acctcttccg
    60301 ccacgcgctc caccagcgca aaacgtgccc cctcgacgtg gctgaccacc gtttctgcaa
    60361 tgtcagcgta gctgaggcaa tccgccacat catcactttt cgccgcttta cggttatccc
    60421 acgccatttc gatatcgaac actaacttct gttcgatggt ctgttcccag tcgtaaacac
    60481 caatagtggt gattaccgaa agttgctcta taaatacaat atccatcacg tcctgcctgc
    60541 tttttggcta accggatacc acttccggcg aaatgtgcgt attatccaca gattcatcgt
    60601 tgaacacgaa ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc
    60661 atcgcgtacc tctgcggctc catttccagt gccattctgg tttgccgctt gtgtgggctg
    60721 cccgatccgc gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt
    60781 ggcaagggag cagccgtagc agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc
    60841 tggggcgcgt atgaattagg tgtcagcccc ttctggctag gcttaattgc catcgccgcc
    60901 tgtcttggac acatctggcc cgttttcttc ggatttaaag gaggaaaagg cgttgctacc
    60961 gcttttggtg ccatcgcacc cattggctgg gatctcaccg gagtaatggc gggaacctgg
    61021 ttactgaccg tgctattgag cggatactcg tcgctgggag cgattgtcag tgcactgatt
    61081 gctccgtttt atgtctggtg gtttaagcca caattcacct tcccggtttc gatgctctct
    61141 tgcctgatcc tgctgcgtca tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca
    61201 aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat
    61261 ctacatgacc tgatagcctt catcgggttt gcccagccgt tgctggcacc atgccgccag
    61321 aaattccacg cagacacgta atttcatgct gcgatagagc ggctcccggt aaacagccca
    61381 gatattggcg ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa
    61441 cggcaacaca tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcag
    61501 cacaatctcg ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg
    61561 accgttcccc aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc
    61621 atgacgactt aattcctgta gggattgtgg ctgcggatat ttttgcagat actcaggtgc
    61681 tgcgcataat attcttttat tttttgtcaa aagatgcgca atataataat caggaatttc
    61741 gtcattaata cgaatatcca gatcaatatt atcctgcact aaatcaattt gtcgatcgaa
    61801 caattcaaaa tgcacctgta actcaggata attgcgcatc agttcggtaa tggctggcgc
    61861 aatatggctg cgcccaaaac caaaactgca accaatacga atcatcccct ccgggcgcgt
    61921 tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg
    61981 ttcgtaacaa cgttgaccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag
    62041 cgttgttgcc aacgtatttt caaggatttg gatccgcttg gtgacaaatg cgggcgtctg
    62101 cccaagcgtc gccgctgcgg cgctaaaact cccgctgtgg acgatttcta ccagtacctg
    62161 caaatctttg gctaaaggcc agctattcag catcgttaat tatccgcagt tgtgataagc
    62221 gcagtgtatt tcgcaaaaca ttgccattcc ctgctctggc gcaatattca tacttatttt
    62281 cgcgttgatt cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat
    62341 ttttagtggt agtttgtggc ggtgaatttt tccagacaaa tacagaaact ggagttgcca
    62401 tgatgagcga aagtaataag caacaggcag tgaataagtt gacggagatt gtcgctaact
    62461 ttaccgccat gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa
    62521 aggatgccga aacgtcgtcg atggggaaaa ttatctacca tacgatgttc gacaacatgc
    62581 aaaaagcgat tgacctgaat cgtcctgcct gtcaggacac cggggagatt atgttcttcg
    62641 ttaaagtcgg ttcccgcttc ccactgcttg gcgagctgca aagcatactc aaacaagccg
    62701 tggaagaggc aaccgtcaaa gcgccactac gtcacaatgc ggtagaaatt tttgacgaag
    62761 tgaacacagg caaaaatacc ggcagcggcg taccgtgggt cacctgggat atcgtccccg
    62821 acggtgacga tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg ctaccaggcc
    62881 gctcgaaagt gttaatgccg tcagaaggct acgaaggcgt ggtgaaattc gtcttcgaaa
    62941 atatctccac cctcgcagta aacgcctgtc caccggtact ggtgggcgtg ggcatcgcca
    63001 cctcggtgga aaccgccgcc gtgctctcgc gaaaagccat tttgcgcccg attggcagcc
    63061 gccaccccaa tccaaaagcg gcagagctgg agctacgcct ggaagaagga ctcaaccgtc
    63121 tggggattgg tccacaaggg ctgactggca acagttcagt gatgggcgtg catatcgaat
    63181 ctgccgctcg ccatccgtca accatcggcg ttgctgtttc tacaggctgc tgggcgcatc
    63241 gtcgcggcac actgctggtt catgccgatc tctcctttga aaatctgtct cacacccgga
    63301 gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct gaagatctgc aagatattcg
    63361 cgtcggcgat gtgatctacc tgaccggtac gctggtgacc tgccgcgacg tttgtcaccg
    63421 ccgcttgatc gaactgaaac gtccgatccc ttacgatctc aacggcaaag cgattttcca
    63481 cgctggcccc atcgtgcgca aaaacggcga caaatgggag atggtctccg tcggtccgac
    63541 aaccagtatg cgtatggaaa gttttgaacg tgaatttatc gagcagaccg gcgtgaaact
    63601 ggtggtcggc aaaggcggaa tggggccgct gaccgaagaa ggctgccaga aattcaaggc
    63661 gctacatgtg attttcccgg caggctgcgc ggtgctggcg gcaacccagg tggaagagat
    63721 tgaagaagtg cactggacag aactcgggat gccggagtca ctgtgggtct gccgggtcaa
    63781 agagttcggc ccgctgattg tctctattga tacccacggc aacaacctga tagccgaaaa
    63841 caaaaagctg ttcgccgaac gccgcgatcc catcgtggaa gagatctgcg agcacgtcca
    63901 ttacatcaaa taaccctccc ggagaggctc gccccctctc ctttttcgca ggcataacac
    63961 gatgaaacct tccactgaat ggtggcgata ccttgcgccg ctggcggtca tcgccattat
    64021 tgctctaatt ccggttcccg cagggctgga gagtcatacc tggctctact ttgccgtttt
    64081 tactggcgta atcgttggac tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt
    64141 gggtatatcc atcatcgcca tactctctcc ctggctgctg ttcagccccg aacagctcgc
    64201 tcagccaggc tttaaattca ctgcaaaatc cctctcgtgg gccgtttccg gtttttctaa
    64261 ttcggttatc tggctgattt tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg
    64321 gctggggcga cgtatcgcgc tgattctggt gaaaaagatg gggcatcgca cgctgtttct
    64381 tggctatgcg gtgatgttct ccgagctgat cctggcacct gtcacaccgt ccaactcggc
    64441 gcgtggtgcg gggattatct atcccatcat ccgtaacctg ccgccgctct atcaatcaca
    64501 accaaacgac agcagttcgc gcagtattgg ctcgtacatc atgtggatgg ggattgttgc
    64561 cgactgcgtg accagcgcca ttttcctgac ggcgatggct cctaacttgc tgttaattgg
    64621 actgatgaaa agcgcatctc acgccacgct gagttggggc gactggttcc tcgggatgtt
    64681 gccgctcagt attttactgg tcctgctggt tccctggctg gcttacgtgc tgtacccgcc
    64741 ggtactgaag tctggtgatc aggtaccgcg ctgggcagag acggaactgc aggcaatggg
    64801 cccgctctgt tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg
    64861 gattttcggc ggtgattata tcgatgccgc gatggtcggt tacagcgtgg tggcgctgat
    64921 gctgcttctg cgcattatca gttgggacga tattgtcagt aataaagcgg cgtggaacgt
    64981 tttcttctgg ctggcctcgc ttatcaccct cgcgaccgga ctcaacaaca ccggttttat
    65041 tagctggttt ggcaaactgt tagcaggcag cttaagcggt tattcgccaa cgatggtgat
    65101 ggtggcgttg attgtggtgt tttatctact gcgctacttt ttcgccagcg ccacggcgta
    65161 tacctccgct ctcgcaccga tgatgatcgc cgccgcgctg gcgatgccgg aaatcccgct
    65221 gccggtgttc tgcctgatgg ttggcgcggc aattggtctg gggagcattc ttacaccata
    65281 cgccaccgga cccagtccga tttactacgg tagtggttat ctgccaacgg cggattactg
    65341 gcgactgggg gcgatttttg ggctgatatt cctcgtgttg ctgatgatta ccggcttact
    65401 gtggatgccc gtggtgttgc tttaagtgag gacggtgccg gaaattgctc tccggcaccc
    65461 aatagtttta cgcagccggt aactccgcca gcggccagcg cggacgcacg ctaacaccga
    65521 gatccgccgt cgcgcctgct ttaaaccgca ccattccggc ataggcgatc atcgcaccgt
    65581 tatcagtaca aaattccgga cgcgcgtaga acacttcgcc gcggcgtttt ttcatcattt
    65641 ccgccagttt cgcccgcagc gtgcggttag cactcacgcc gcccgccatg accagtcgct
    65701 taaagcccgt ctgatccagt gctcgcttac acttaatcat caacgtatca accaccgcat
    65761 cttcaaaggc gcgggcgata tcagcacgcg tctggtcgtc ggtgccgttg tcacgaatcg
    65821 tgttcgccgc aaaggttttc agaccagaaa agctgaaatc cagccccgga cggtcggtca
    65881 tcggacgcgg gaaaacaaag cgcccggcag taccctgagc cgccattttc gacagtaacg
    65941 gtccgccagg ataatccagc cccagcagct tcgcggtttt atcaaacgct tcaccggcgg
    66001 catcatcgat agactcgccg agcagctcgt actgaccaat gccagtcacg ctgattaact
    66061 gcgtatgacc gccggaaacc agcagcgcaa caaacggaaa ttccggcggg ttatcttcca
    66121 gcatcggcgc taacagatgc ccttccatat ggtgtacagg gatcgccgga acgttccagg
    66181 caaacgccag agaacgcccc acggtcgcgc caaccagcag tgcgccgact aatccagggc
    66241 ctgcggtata ggccacagca tcaatgtctt ttgccgttaa accagactcc tttagcgccg
    66301 cctggatcaa cggtacggtt ttacgcacat gatcgcggga ggccagctca ggcacaacgc
    66361 ccccgtagtc agcgtgcaat ttcacctgac tatacaattg gttggctaac aaaccttttt
    66421 catcgtcgta aatggcgatg ccggtttcat cgcaggaagt ttcaataccc agtacacgca
    66481 tgacttgttt tacctcgctt tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa
    66541 actttgttcg cccctggaga aagcctcgtg tatactcctc acccttataa aagtcccttt
    66601 caaaaaaggc cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt
    66661 tgaaataagc tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat
    66721 gccggtaatt aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg
    66781 ttcctgcgaa aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc
    66841 gactaccgaa cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc
    66901 tcgcgaaaac gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc
    66961 agaccgagtt gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt
    67021 atgaattgct aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat
    67081 ctgctggcac gcaccgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag
    67141 ggcaagaatt tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt
    67201 aacggtgaga aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac
    67261 ttcctgatga actacgacaa gctcgagttc gtcgaaacgg tcgaagagct ggcagcaatg
    67321 cacaatcttg aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag
    67381 cggcaaacgc tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacag
    67441 caacctgttg ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt
    67501 atcgctcgct ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt
    67561 ggcggcaatc cagaaaatcg ccagtcattg attgatgcgg gcatgttggt cactaacgat
    67621 cagggacgca gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc
    67681 ggtcgggtga ttggttttgg cgggcgtgtg ctgggcaacg atacccccaa atacctgaac
    67741 tcgccggaaa cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag
    67801 caggataacg ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg
    67861 ctggcgcaat acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat
    67921 cacatacaac tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt
    67981 gcaggccgtg atgccgcatg gcgagcgctg gaaacggcgc tgccttacat gacagacggc
    68041 cgtcagctac gctttatgtt tttgcctgat ggcgaagacc ctgacacgct ggtacgaaaa
    68101 gaaggtaaag aagcgtttga agcgcggatg gagcaggcga tgccactctc cgcatttctg
    68161 tttaacagcc tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt
    68221 acgctggcac tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt
    68281 caggaattag gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca
    68341 aaagcggcag agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt
    68401 atacttatag ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag
    68461 aatctggatg aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt
    68521 ctctcccagc caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat
    68581 gctgccaccc ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag
    68641 caaaccttca ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa
    68701 gagttaatcg ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg
    68761 acattaaacc aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg
    68821 ggaagccccc gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat
    68881 tatcgttggc ggtaaataac cgttggattt cagcgttaac ggctgaagga catcgggtca
    68941 atcgcccaac accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc
    69001 agtcacagct gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg
    69061 aggtcaatga ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc
    69121 aaatgatcaa cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga
    69181 tgctggctga aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca
    69241 gcgtggaatc tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg
    69301 gcaccgttga actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg
    69361 ggatcaacca ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg
    69421 aacagtacga tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg
    69481 ttgacccgaa cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt
    69541 cccaggaaga tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg
    69601 ccgatgatga caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactgcgcg
    69661 ctcagtacgt tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgctgctc
    69721 aggaagagat cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt
    69781 ttgactacct ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc
    69841 tgatcatgaa gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt
    69901 ttaccggcaa cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt
    69961 ggtcggaaaa actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc
    70021 agattgaaga agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt
    70081 ccatcggtga agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc
    70141 tggttatttc tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc
    70201 aggaaggcaa catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca
    70261 agttctccac ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc
    70321 aggcgcgcac catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta
    70381 tttctcgcca gatgctgcaa gagatgggcc gtgaaccaac gccggaagaa ctggctgaac
    70441 gtatgctgat gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct
    70501 ccatggaaac gccgatcggt gatgatgaag attcgcatct cggggatttc attgaggata
    70561 ccaccctcga gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacacacg
    70621 acgtgctggc tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg
    70681 atatgaacac cgatcacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac
    70741 gtatccgtca gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag
    70801 tgctgcgtag cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg
    70861 gcacctgtcc ctttgcacaa acgccacctt ttcggtggcg ttttttatcg cccacgcacc
    70921 accagcgcct ggtccagctc gcgatacgct tcaaccagtt tctccagtga aacgcgactt
    70981 aaaccactgg gatttggcag cacccaaatc tgcgtcgaac caatgctgag cgtttgtttc
    71041 ccccactgtg caccgcgctg gctgaatccc tgttcatatg cttgtttacc cagaatcgcc
    71101 aacgcttgcg gctgataatc ttcaattttt tcaatcagct tacgcccacc tgcgtgtagc
    71161 tcttgctttg aaatttcatt ggcttgcacc gttggacggt ctaccagttt ggtgacgcca
    71221 caacgataat ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg
    71281 gcctgatata tcaccttcca gaagcgattt gccggatgag caaagggaaa accagtcccg
    71341 gcggatgaaa gcccagggtt gataccgcaa aacacgaccc gtaaccctgg agccaaaata
    71401 tcctcaacca tatttactcc tttctaacat catcaggaaa agtataacgg attgaaaata
    71461 cattgtttat aaaaacagca ggcgcgcggt aatggctgga ttgcgacacg gagttacttt
    71521 ataatccgct accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg
    71581 gtcgctggtt caagtccagc aggggccacc agatatagca aaggctgacg agaaatcgtc
    71641 agcctttttc tttttatata tcagttactt tgcgtgccag taagccgctg cacgtacccg
    71701 ctgtgggtca tactgttccg cttcaaagca gcggcttaaa ttcttaacga ctttaccttc
    71761 gccggttatc cagatgaagt aatcatcggc agggatttgc atctgcgcca gacgcgcatc
    71821 taccgcctgc tcatcatgtg ccagccattc gatattaaaa ccatcaaggt gcgcgagata
    71881 atcctgacag gcgttatccc gcacgctaac cagcgcacta acttgcggtt taacggcaag
    71941 tttgctcaac gtttccaggc ggcggcgcaa tgcgggcatt ccggattcat cgcagacata
    72001 cagctgatac gcgtaatctt ccggcaccac cagcgaaccg cgcggacctg ccaccgtgag
    72061 tttatcgccc ggttgggcct gcattgccca gctactggcg acgccgtctt cgtgaataaa
    72121 gaaatcaatc gccagttcat ggcgtagttc gtcatacagt ggcgtatagt cacgcgacgg
    72181 tgggcgtggt ccttccggcc agacgatgcc ctcttccgtt accgttggcg gcacaaagtg
    72241 agcgtcaggt tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg atgtaaaacc
    72301 gtccagcgcc tcgccgccaa ggacaatgcg ctgaaaaccg gcgctgatac gctcaacgcg
    72361 tagcacagtc agttcacgga accgcagatc attgcgaacg cgctgcgggt agcgggggga
    72421 gttattcatt gttatcgcct tcgtgatggt aatcagatat atctaaataa ttttcgcaaa
    72481 tgataatgat tgttaatcat gataaatgca agcgatttgt agaactgata tatctatagt
    72541 ctgataagac gaaccgcctc ttctcaggca tcattactca acgccggatg cggcgtgaac
    72601 gccttatccg gcctacgtgt gagatgagtc cacatttaaa aatcattttt attcttgcaa
    72661 gagtgctcat atcagatata aattagatat atctaattaa gcaaaaggag gctgatatga
    72721 gccatcatca cgaagggtgt tgtaaacatg aaggccagcc acgccatgag ggctgctgca
    72781 aaggtgagaa gtcagaacac gagcactgcg gacacggtca ccagcatgaa cacggtcaat
    72841 gttgcggtgg tcgccacggt cgcggcggcg gtcgtcggca acgtttcttt ggtcacggtg
    72901 aattacgtct ggtgattctg gatattctct cgcgcgatga cagccacggt tacgaattga
    72961 ttaaagcgat tgagaatcta acccagggga attacacccc aagcccgggc gtcatctacc
    73021 caacgctgga ttttctgcag gagcagtcgc tgattaccat ccgcgaagag gaaggtggta
    73081 agaagcagat tgcactgacc gaacaaggcg cgcagtggct ggaagaaaac cgcgaacagg
    73141 tggagatgat tgaagaacgc atcaaagcgc gttgcgttgg tgctgcgctg cgccagaacc
    73201 cgcaaatgaa gcgggcgctg gataatttta aagcggtgct ggatttacgc gtcaaccaga
    73261 gtgatatcac tgatgcacaa ataaaaaaga tcattgcggt gatcgaccgc gccgcttttg
    73321 atattacgca actggattaa tcaccgattg ccggatgcgg cgtaccctac ataccccgta
    73381 ggcctgataa gatgcgccag catcgcatca ggcattgtgc tccaaccgcc gaatcctgca
    73441 taccgattaa tgcagcaccg tcaccgcgtc ttccagtcgg ctggcgcggt gtttcaccat
    73501 ctccgacacc tgcgcactct cttccaccag ctcggcattt ttctgggtga tgaggttaag
    73561 ctcatccact gcacgggtca ggctggaaag accatcggcc tgttccagcg ttgaatggct
    73621 aatctgggca atcaactggg tgacgttttt cacctgtgcc acaatatctt ccatcgtacg
    73681 tccggcggcg tgtacctgct gcgaaccgga ttgcacctta tcagcactgg catcaatcag
    73741 cttgcgaata tcgttggcag cattggcgct gcggctggct aaatgacgca cttcccccgc
    73801 caccactgca aaacctttgc cctgttcgcc ggctcgcgcc gcttccaccg ccgcattcag
    73861 ggccagaata ttggtctgaa acgcaatatc gttaatcagc gaagtaatgg tgccaatgcg
    73921 ctgggtactg tcggcgatat cgtccatagt cttgatcacc gtggtcatcg cctccccgcc
    73981 ctgcactgcc gcattactgg cagtgattga cagtttatcg gcagccgacg ccgtggcaga
    74041 gttctgtttc accgacgccg ccatttggtt catggtcgcc accgtttgct gaacgttatc
    74101 aactgtctgc tgggtatgtt cgttcagttc atcggtgcct ttcgccagcg tctcactgcc
    74161 atttctgaca ctggacacct ggcttgagac atcgttaatt agccaacggc acatcaggcc
    74221 aagctgccct accgcgcgta atgtcagccc cagctcatcg ctgcgattca gatgctcaac
    74281 actattacgc tctccggtcg ccaccttcag tgcctgacgg gcgacatttt ctatcgggcg
    74341 gacaatctgc cattcaaaac aggcgcttgc caacaatacc actaacgcac agaggatata
    74401 cgtcaccacc ggggcagcaa caaaccaaag catggccgcc agcaagataa acatcagggt
    74461 catcactcca cgcgcccgcc agcgaagcgg taatgaaggc agtttaccca gccagccttt
    74521 acgcaccacc aggcctttat gaatacgctt accggtacgt ccggcgttca gcgctttgta
    74581 cagcggctcc accgccgcga tctcttcatc cgtcgcccgg gtacgaatcg acatatagcc
    74641 actgattttt ccctcgcgca ccatcggtac cgcattggcc cgcacccaat aatggtcacc
    74701 atttttgcgg cgatttttca cgatgccgct ccagggctcc ccttttttca gggtgaacca
    74761 catatctgca aacgccgctt ttggcatatc cgggtgacgc accatgttgt gcggctgccc
    74821 ttgtaactct tgcaaggtat agccgctcac ctgcacaaaa gtgtcattag catgagtgat
    74881 atagctttgc agatcggtag tggacatcag agtggtatcg tccgccagcg gggtattttg
    74941 ctgggtgaca tacggatgag aagacatgtt cgcgtcctgt gcaggttata tggttgttaa
    75001 cttcttgtca gagtttatgt cggccccgct gcggttatct ttaaccgatt aatttgattt
    75061 agatcgcaat ttgcgattaa agcacaaatc taattcgttg atttaaaata ctttcactct
    75121 gttactatac gaaaacgtta attatcttgc ccaaaaatca ggcaattatt gccctgaaaa
    75181 cgtgcatttg cgcagcaatt atcaaatcca tacccgacaa aaaccgtgca aaataacaac
    75241 ggatgttaac aaatagcatt aaatattgcg caaatgataa ccacatttgt gtttatcccg
    75301 attttcgcga tcgcagccgg agtggcgcaa tccctgcaat acttaaatcg gtatcatgtg
    75361 atacgcgagc ctccggagca tattttgaac aggttacctt cgagcgcatc ggctttagcg
    75421 tgcagcgccc acgccctgaa tctcattgag aagcgaacgc tggatcatga ggagatgaaa
    75481 gcacttaacc gagaggtgat tgaatacttc aaagagcatg tcaatccggg gtttttagag
    75541 tatcgcaaat ctgttaccgc cggcggggat tacggagccg tagagtggca agcgggaggt
    75601 ttaaatacgc ttgtcgacac ccagggacag gagtttatcg actgcctggg aggttttgga
    75661 attttcaacg tggggcaccg taatccagtt gtggtttccg ccgtacagaa tcaacttgcg
    75721 aaacaaccac tgcacagcca ggagctactc gatccgttac gggctatgct ggcgaaaacc
    75781 cttgctgcgc taacgcccgg taaactgaaa tacagcttct tctgtaatag cggcaccgag
    75841 tccgttgaag cggcgctgaa gctggcgaag gcttatcagt cgccgcgcgg caaatttact
    75901 tttattgcca ccagcggcgc gttccacggt aaatcacttg gcgcgctgtc ggccacggcg
    75961 aaatcgacct tccgcaaacc gtttatgcca ctgctgccag ggttccgtca tgtgccgttt
    76021 ggcaatatcg aagccatgcg cacggctctt aatgagtgca aaaaaaccgg tgatgatgtg
    76081 gctgcggtga tcctcgaacc gattcagggc gaaggtggcg taattctgcc gccgccgggc
    76141 tatctcaccg ccgtgcgtaa gctgtgcgat gagttcggcg cactgatgat cctcgatgaa
    76201 gtacaaacgg gcatggggcg cacgggcaag atgtttgcct gcgaacatga gaacgtccag
    76261 ccagacattc tgtgtctggc aaaagccctc ggcggcggcg tgatgccgat tggcgcaacc
    76321 atcgctactg aagaggtgtt ctcagtcttg ttcgacaacc cgttcctgca taccaccacc
    76381 tttggcggca acccgctggc ctgtgcggcg gcgctggcga ccatcaatgt gttgctggaa
    76441 caaaacttac cggctcaggc tgaacaaaaa ggcgatatgt tgctggacgg tttccgtcaa
    76501 ctggcgcggg aatatcccga tctggtgcag gaagcacgtg gtaaagggat gttgatggcg
    76561 attgagtttg ttgataacga aatcggctat aactttgcca gcgagatgtt ccgccagcgc
    76621 gtactggtgg ccggaacgct caataacgcc aaaacgatcc gcattgaacc gccactgaca
    76681 ctgaccattg aacagtgtga actggtgatc aaagcggcgc gtaaggcgct ggcggccatg
    76741 cgagtaagtg tcgaagaagc gtaatatcaa atcggatggc gatacgacgt cgccatccga
    76801 tctttttata cgatacgtac gcccgcaggc atcatccgct ccggcgttaa cagcacgctt
    76861 tcactgccgt catcggtttc cgcacacaac agcatgcatt ccgacgtttc accgcgcatt
    76921 ttcgcttttt gcagattgca taagaccacc accgtttttc ccatcaactc ttcttcgctg
    76981 tagtacggca ccaggctggt cacggtttgc agtgtttttt cccccacatc aacctgtacg
    77041 atgtacagct tgtcggcgtt ttcatggcgt ttcacttcca caatctttcc gacgcgcatt
    77101 tccagacgtg caaaatcagc gtaagccacg gtttccattg cttcctccct tcgagtaaaa
    77161 ttttactaaa ctatagcaaa agtttttctc aatcctgtag gctaaaaatg gagaatgcag
    77221 gcgtgatcac attcgtgagc cgctgtgtta ccgttacagc gtcaaagaaa cgcgctttat
    77281 ttactgaaaa caggtgaccc gataagcact tcctctacaa tgggggcgca catcagggaa
    77341 agtaaaaaag gtaaacatgg caacactaaa agacatcgca atcgaagctg gcgtatccct
    77401 ggcgacagta tccagggtct taaatgacga tccgacattg aatgtgaaag aagagacgaa
    77461 acatcgcatt ctcgagatcg ccgaaaagct ggagtacaag accagtagcg cccgtaaact
    77521 acagacaggc gcagtcaacc aacaccatat tctggctatc tacagctacc agcaggagct
    77581 ggagatcaac gatccttact atctggcgat ccgccacggc attgaaactc agtgcgaaaa
    77641 gctggcgatc gaactgacca actgttatga acacagcgga ttaccagaca ttaaaaacgt
    77701 caccggtatt ttaattgtcg gcaaacccac acccgccctg cgcgccgccg cctgtgcgtt
    77761 gaccgacaat atctgtttta tcgactttca cgaacccggc agcggttacg atgcggtgga
    77821 tatcgatctg gcacgcatca gtaaagaaat catcgacttc tatatcaacc agggcgttaa
    77881 tcgcattggt tttattggcg gtgaagatga gcctggcaag gcggatattc gtgaggtcgc
    77941 ctttgcggaa tatggccgtc tgaaacaagt agtacgcgaa gaggatatct ggcgcggcgg
    78001 tttttccagt tcgtcgggtt atgaactggc aaaacagatg ctggcgcggg aagactatcc
    78061 gaaggcactg tttgttgctt ccgattccat tgctatcggc gtactgcggg caattcatga
    78121 acgtggcctg aacatcccac aggatatttc gcttatcagc gttaacgata tccccaccgc
    78181 gcgatttacc tttccgccgc tctccaccgt gcgcatccat tccgagatga tgggaagtca
    78241 gggcgttaac ctggtgtatg aaaaagcccg cgatggtcgc gcgctgccgc tgttagtctt
    78301 cgttcccagc aaattaaaac tgcgcggcac gacccgttaa atccccttac acactgtccg
    78361 gcaatcattt ttgccggaca gtgctgccgt ttattttcgt gatccagtta aagtaaatgc
    78421 atttacctgc tattttttag taaaaatttt actaaactcc ccagcaatta cacaaactac
    78481 catcaccatg aatggttccg atttctctct accgggaggc cctatgaatc gctgggaaaa
    78541 cattcagctc acccacgaaa accgacttgc gccgcgtgcg tacttttttt catatgattc
    78601 tgttgcgcag gcgcgtacct ttgcccgcga aacgagcagc ctgtttctgc ccttaagcgg
    78661 tcagtggaat ttccactttt ttgaccatcc gctgcaagtg ccagaagcct tcacctctga
    78721 gttaatggct gactgggggc atattaccgt ccccgccatg tggcaaatgg aaggtcacgg
    78781 caaactgcaa tataccgacg aaggttttcc gttccccatc gatgtgccgt ttgttcccag
    78841 cgataaccca accggtgcct atcaacgtat tttcaccctc agtgacggct ggcagggtaa
    78901 acagacgctg attaaatttg acggcgtcga aacctatttt gaagtctacg ttaacggtca
    78961 gtatgtgggt ttcagcaagg gcagtcgcct gaccgcagag tttgacatca gcgcgatggt
    79021 taaaaccggc gacaacctgt tgtgtgtgcg cgtgatgcag tgggcggact ctacctacgt
    79081 ggaagaccag gatatgtggt ggtcggcggg gatcttccgc gatgtttatc tggtcggaaa
    79141 acaactaaca catattaacg atttcaccgt gcgtaccgac tttgacgaag cctattgcga
    79201 tgccacgctt tcctgcgaag tggtgctaga aaatctcgcc gcctcccctg tcgtcacgac
    79261 gctggaatat accctgtttg atggcgaacg cgtggtgcac agcagcgcca ttgatcattt
    79321 ggcaattgaa aaactgacca gcgccagctt tgcttttact gtcgaacagc cacagcaatg
    79381 gtcagcagaa tccccttatc tttaccatct ggtcatgacg ctgaaagacg ccaacggcaa
    79441 cgttctggaa gtggtaccac aacgcgttgg cttccgtgat atcaaagtgc gcgacggtct
    79501 gttctggatc aataaccgtt atgtgatgct gcacggcgtc aaccgtcacg acaacgatca
    79561 tcgcaaaggc cgcgccgttg gaatggatcg cgtcgagaaa gatctccagt tgatgaagca
    79621 gcacaacatc aactccgtgc gtaccgctca ctacccgaac gatccgcgtt tttacgaact
    79681 gtgtgatatc tacggcttgt ttgtgatggc ggaaaccgac gtcgaatcgc acggctttgc
    79741 taatgtcggc gatatcagcc gtattaccga cgatccgcag tgggaaaaag tctacgtcga
    79801 gcgcattgtt cgccatattc acgcgcagaa aaaccatccg tcgatcatca tctggtcgct
    79861 gggcaatgaa tccggctatg gctgtaacat ccgcgcgatg taccacgcag cgaaagcgct
    79921 ggatgacacg cgactggtgc attacgaaga agatcgcgat gctgaagtgg tcgatattat
    79981 ttccaccatg tacacccgcg tgccgctgat gaatgagttt ggtgaatacc cgcatccgaa
    80041 gccgcgcatc atctgtgaat atgctcatgc gatggggaac gggccgggcg ggctgacgga
    80101 gtaccagaac gtcttctata agcacgattg cattcaggga cattatgtct gggaatggtg
    80161 cgaccacggg atccaggcgc aggatgacaa cggcaatgtc tggtataaat tcggcggcga
    80221 ctacggcgac tatcccaaca actataactt ctgtcttgat ggtttgatct attccgatca
    80281 gacgccggga ccaggcctga aagagtacaa acaggttatt gcgccggtaa aaatccacgc
    80341 gctggatctg actcgcggcg agttgaaagt cgaaaataaa ctgtggttta ccacgcttga
    80401 tgactacacc ctgcacgtag aagtgcgcgc cgaaggtgaa acgctcacga cgcggcagat
    80461 taaactgcgc gacgttgcgc cgaacagcga agcccccttg cagatcacgc tgccgcagct
    80521 ggacgcccgc gaagcgttcc tcaacattac ggtgaccaaa gattcccgca cccgctacag
    80581 cgaagccggg cattctatcg ccacttatca gttcccgctg aaggaaaaca ccgcgcagcc
    80641 agtgcctttc gcaccaaata atgcgcgtcc gctgacgctg gaagacgatc gtttgagctg
    80701 caccgttcgc ggctacaact tcgcgatcac cttctcaaaa atgagtggca aaccgacatc
    80761 ctggcaggtg aatggcgagt cgctgctgac ccgcgagcca aagatcaact tcttcaagcc
    80821 aatgatcgac aaccacaagc aggagtacga agggctgtgg caaccgaatc atttgcagat
    80881 catgcaggaa catctgcgcg actttgccgt agaacagagc gatggtgaag tgttgatcat
    80941 cagccgcacg gttatagcac cgccggtgtt tgacttcggg atgcgctgca cctacatctg
    81001 gcgcatcgct gccgatggcc aggttaacgt ggcgctttcc ggcaagcgtt acggcgacta
    81061 tccgcacatc attccgtgca tcggtttcac catggggatt aacggcgaat acgatcaggt
    81121 ggcgtattac ggtcgtggac cgggcgaaaa ctacgccgac agccagcagg ctaacatcat
    81181 cgatatctgg cgcagcaccg tcgatgccat gttcgagaac tatcccttcc cgcagaacaa
    81241 cggtaaccgt cagcatgtcc gctggacggc actgactaac cgccacggca acggtctgct
    81301 ggtggttccg cagcgcccaa ttaacttcag cgcctggcac tatacccagg aaaacatcca
    81361 cgctgcccag cactgtaacg agctgcagcg cagtgatgat atcaccctga atctcgacca
    81421 ccagctgctt ggcctcggtt ccaattcctg gggcagcgag gtgctggact cctggcgcgt
    81481 ctggttccgt gacttcagct acggctttac gttgctgccg gtttctggcg gagaagctac
    81541 cgcgcaaagc ctggcgtcgt atgagttcgg cgcagggttc ttttccacga atttgcacag
    81601 cgagaataag caatgaggat catcgataac ttagaacagt tccgccagat ttacgcctct
    81661 ggtaagaagt ggcaacgctg cgttgaagcg attgaaaata tcgacaacat tcagcctggc
    81721 gtcgcccact ccatcggtga ctcattgact taccgcgtgg agacagactc cgcgaccgat
    81781 gcgctattta ccgggcatcg acgctatttt gaagtgcatt actacctgca agggcagcaa
    81841 aaaattgaat atgcgccgaa agagacatta caggtagtgg aatattatcg tgacgaaact
    81901 gaccgtgaat atttaaaagg ctgcggagaa accgttgagg tccacgaagg acaaatcgtt
    81961 atttgcgata tccatgaagc atatcggttt atctgcaata acgcggtcaa aaaagtggtt
    82021 ctcaaagtca ccatcgaaga tggttatttt cataacaaat aacaactacg gcggcaaaag
    82081 gagtttgccg ccaccgctac cctactcatt ttcggagatg tgttatgtct gataccaaac
    82141 gtaatacaat cggcaaattc ggcttgctct cgctgacttt tgccgccgtt tacagcttta
    82201 acaacgttat caataataat attgagcttg gactggcctc ggcaccgatg tttttcctcg
    82261 cgacgatttt ttattttatt cccttctgtc tgatcatcgc agaatttgtt tcgttaaata
    82321 aaaactcaga agccggtgtc tacgcgtggg taaaaagttc gctgggcgga cgttgggcat
    82381 ttattactgc ctatacctac tggttcgtaa acctgttctt tttcacctcg ctgttgccgc
    82441 gcgttattgc ttatgcttcg tatgccttcc tcggctacga atatattatg acgccggttg
    82501 ccaccaccat tatcagtatg gtgctgttcg ccttctctac ctgggtttcc accaacgggg
    82561 cgaaaatgct ggggccaatt acctccgtca cttcaacgct gatgctgctg ttaacgctct
    82621 cctacatttt actggcaggt acggcgctgg ttggcggcgt acagcctgct gaccccatca
    82681 ccgttgacgc gatgatcccg aacttcaact gggcgttcct cggcgttacc acctggattt
    82741 ttatggccgc aggtggcgcg gagtccgtcg ctgtgtacgt taacgacgtc aaaggcggtt
    82801 cgaaatcgtt cgttaaagtg atcatcctcg ccgggatttt tatcggcgta ctatattccg
    82861 tctcctcggt gctgattaac gtcttcgtca gcagcaaaga gttgaaattt actggcggat
    82921 cggtgcaggt attccacggc atggcggcgt attttggtct gccggaagcg ttgatgaatc
    82981 gctttgtcgg tctggtgtcc tttaccgcaa tgttcggttc cctgctgatg tggaccgcaa
    83041 cgccggtgaa aattttcttc tccgaaatcc cggaaggcat ctttggtaag aaaaccgtag
    83101 aactgaacga aaacggcgtt ccggcgcgcg cagcgtggat ccagttcctg atcgtcatcc
    83161 cgctgatgat tatcccgatg ctcggttcca ataccgtgca ggatctgatg aataccatta
    83221 ttaatatgac cgccgcagcg tccatgcttc cgccgttatt catcatgctg gcttacctga
    83281 atttacgcgc caaattagat cacctgccac gcgatttccg tatgggctcc cgccgcaccg
    83341 gtattatcgt tgtttcaatg ctgattgcga tatttgccgt agggtttgtc gcttcgacat
    83401 tcccgactgg cgcgaatatt ctgaccatca ttttttataa cgtcggcggt attgttatct
    83461 tccttggctt tgcgtggtgg aaatacagta aatatataaa gggattaacg gctgaagagc
    83521 gccatattga agcgacgcca gccagcaatg ttgattaaac agactaataa aaaatgagca
    83581 ggcattttcc ctcttcctgg atgaggagag ggaaaatagt ttctgccttt atatttttat
    83641 aattacaacg ataaaaggct gtactttttc tttagctcat ggattaacac aatgaaatta
    83701 atcactgcac catgcagagc attacttgct ctgccgtttt gctacgcctt ttctgcggca
    83761 ggagaagaag cacgtccggc agaacatgac gacacaaaaa cacccgcaat tacctcgaca
    83821 tcttctcctt catttcgttt ttacggcgaa ttaggggttg gtggatatat ggatttagag
    83881 ggtgagaata aacataaata cagcgacggc acctatattg aaggtggcct ggagatgaag
    83941 tacggctcct ggttcggcct gatttacggc gaaggctgga ccgtgcaggc cgaccacgac
    84001 ggcaatgcct gggtgcctga ccatagctgg ggcggtttcg aaggtgggat taaccgtttc
    84061 tatggcggtt atcgaaccaa tgatggcacc gaaatcatgc tcagtctgcg tcaggattcc
    84121 tcgctggatg acctgcaatg gtggggcgac ttcacacccg atctgggcta cgtcattccc
    84181 aatacccgcg acattatgac cgcgctgaag gtgcagaact taagcggcaa ctttcgttat
    84241 agcgtcaccg ccacacctgc cggacatcat gacgaaagca aagcatggct acattttggc
    84301 aagtacgatc gctacgacga caaatacacc tatccggcaa tgatgaacgg ttacatccag
    84361 tatgaccttg ccgaaggcat cacctggatg aatggtctgg aaattaccga cggcacagga
    84421 caactctatc tcacgggcct gcttacccct aactttgccg ctcgcgcctg gcaccatacc
    84481 ggacgtgccg acgggttgga cgtaccggga agtgaaagcg ggatgatggt gagcgccatg
    84541 tatgaagcgt taaagggcgt ttatctctcc accgcttaca cctacgccaa acatcgccct
    84601 gaccacgctg acgatgaaac cacctctttc atgcagtttg gtatctggta cgaatatggc
    84661 ggcggacgtt tcgcaacggc ttttgatagc cgcttctaca tgaaaaatgc ctctaacgat
    84721 cccagcgacc aaatcttcct gatgcaatat ttctactggt aataaggact gtaatttcat
    84781 gaaaatcaaa actattttaa cgccagtaac ctgcgctctg ctgataagtt tttccgccca
    84841 tgccgctaac gccgacaatt ataaaaacgt gattaaccgt actggtgcgc cgcagtacat
    84901 gaaggattac gattacgacg atcaccagcg ttttaatccg tttttcgatc tcggagcctg
    84961 gcatggtcat ctgttgccag acggccctaa caccatgggc ggttttccag gcgttgcgct
    85021 gctgacggaa gagtacatca actttatggc cagcaatttc gaccgcctga ccgtctggca
    85081 ggacggcaag aaagtcgact tcacgctgga ggcatacagt attcccggcg cgctggtgca
    85141 aaaactgaca gcaaaagatg tgcaggtcga aatgactctg cgcttcgcca cgccgcgcac
    85201 gtcactactg gaaactaaaa tcatcagcaa taaaccgctg gatctggtgt gggatggcga
    85261 actgctggaa aaactggaag cgaaagaagg gaaaccgctt tccgataaaa ccattgctgg
    85321 cgaataccct gactaccagc gcaaaatcag cgccacccgt gatggcctga aagtcacctt
    85381 tggcaaagtg cgcgccactt gggatctgct gacctccggt gaatcagaat atcaggtgca
    85441 taaatccctg ccggtgcaga ctgaaatcaa cggtaatcgc tttaccagta aggcgcatat
    85501 caacgggtcg acgacgctct ataccaccta ctcccacctg ctgaccgctc aggaagttag
    85561 caaagagcaa atgcagatcc gcgatattct ggcgcgtccg gcgttttacc tcaccgcctc
    85621 gcagcaacgc tgggaagagt atctgaagaa agggttaacc aatccggatg cgacgccgga
    85681 acagacgcgc gtcgcagtaa aagcaatcga aacgctcaac ggtaactggc gttcgcctgg
    85741 cggggcggtg aaatataaca ccgtcacgcc gtcggtgacc gggcgctggt tctccggcaa
    85801 tcagacctgg ccgtgggata cctggaagca ggcgtttgcg atggcgcatt tcaatccgga
    85861 catcgccaaa gagaatatcc gcgcggtctt ctcctggcag atccagcctg gcgacagcgt
    85921 gcgtccgcag gatgtgggct ttgtccccga cctgatagcg tggaacctta gccccgagcg
    85981 tggtggcgat ggcggcaact ggaacgaacg taataccaaa cccagccttg ccgcctggta
    86041 ggtgatggaa gtgtataacg tcacccagga taaagcctgg ttggcagaga tgtacccgaa
    86101 actggtggcc tatcatgact ggtggttacg taaccgcgat cataacggca acggcgtgcc
    86161 ggaatatggc gcgacccgcg acaaagccca caacactgag agcggcgaga tgctgtttac
    86221 ggtgaagaaa agcgacaaag aagagacgca gtctgggctg aacaactacg cccgcgtggt
    86281 ggagaaaggc cagtacgaca gtctggaaat tccggcacag gttgctgcat cgtgggaatc
    86341 ggggcgtgat gatgccgccg tctttgggtt tatcgacaaa gaacagctgg ataaatatgt
    86401 cgcaagcggc ggcaaacgta gcgactggac ggtgaaattc gccgaaaacc gcagtcagga
    86461 cggaacgttg ctggggtact cgctattgca ggagtcggtg gatcaggcca gctatatgta
    86521 cagcgataac cattatctgg cggagatggc gacgattctc ggtaaaccgg aagaggctaa
    86581 gcgctatcgc cagttggcac agcagctcgc ggactacatc aacacctgta tgttcgaccc
    86641 ggctacgcag tacttctatg acgtgcgtat tgaagataaa ccgctggcga acggctgcgc
    86701 gggcaaaccg attgttgagc gcggtaaagg gccggaaggc tggtcgccgc tgtttaacgg
    86761 tgcggcaacg caggccaatg ccgacgcggt ggtgaaggtg atgctcgatc ctaaagagtt
    86821 caataccttt gttccgctgg gaacggcggc gttaaccaat ccggcttttg gcgctgatat
    86881 ctactggcgc gggcgcgtat gggtggatca gttctggttt ggtctgaaag ggatggagcg
    86941 ttacggttat cgtgatgatg ccctgaagct ggcggatacg ttcttccggc atgccaaagg
    87001 attaaccgcc gatggcccaa ttcaggagaa ttacaacccg ctgactggcg cgcagcaagg
    87061 cgcaccaaat ttctcctgga gtgccgcaca tttgtatatg ttgtataacg attttttccg
    87121 taagcaataa tttgcaacgg ctggcggatg cggcgtgaac gccttatccg ccctacatat
    87181 tcgttcccgt aggctggata agacgcgaca agcgtcgcat ccggcatctg caccgcgcca
    87241 ccggcggatg ctgcgtgaac gccttatccg ccctacatgt tcgttcccgt aggctggata
    87301 agacgcgaca agcgtcgcat ccggcatctg cacctcgcca ctggcggatg cggcgtgaac
    87361 gccttatccg ccctacatgt tcgttcccgt aggctggata agacccgaca agcgtcgcat
    87421 ccggcgtctg caccgcgcca ctggcggatg cggcgtgaac gccttatccg ccctacatgt
    87481 tcgttcccgt aggctggata agacgcgaca agcgtcgcat ccggcatctg caccgcgccg
    87541 gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccagg tcggatagga
    87601 cccggcaagc gtcgcatccg gcatctgcac cgcgccggcg gatgcggcgt gaacgcctta
    87661 tccgccctac atgttcgttc ccgtaggctg gataagacgc gacaagcgtc gtatccggca
    87721 tctgcaccgc gccactggcg gatgcggcgt gaacgcctta tccgccctac atgtgcaatc
    87781 ccccaggtcg gataggaccc ggcaagcgtc gcatccggcg ttaccaccgg gcgtattctt
    87841 tttgaatccc atcacaaacc ccgcactccc cttttccctt ttctccggcg acggctaaat
    87901 tagaactcat ccgaccacat aacaattatt ttacatactg gacaatttta tgagctaccc
    87961 gtcgctgttc gccccgctgg atttaggttt taccacgtta aaaaaccgcg tgttgatggg
    88021 ttcaatgcac accgggctgg aggaataccc ggacggcgct gaacggctgg ctgcgtttta
    88081 tgccgaacgc gcccgtcacg gcgtggcgct gattgttagc ggcggcattg caccagattt
    88141 aacaggcgtt ggcatggaag gcggcgcaat gctcaatgac gccagccaga tcccacacca
    88201 tcgcaccatt accgaagccg tacatcagga aggcggcaaa atagccctgc aaattttgca
    88261 taccgggcgc tacagctacc aaccgcatct ggtcgcaccg tccgcattgc aggcccccat
    88321 caaccgtttc gttcctcatg aactcaccca tgaagagatc ctgcaactga tcgacgattt
    88381 cgcccgctgc gcgcaactgg cgcgggaggc aggatacgac ggtgtagagg tgatgggttc
    88441 cgaagggtat ttgatcaacg aatttctgac gctgcgcacc aatcagcgta gtgaccagtg
    88501 gggcggcgat taccacaacc ggatgcggtt tgccgttgaa gtggtgagtg cggtacgcga
    88561 acgcgttggc aacgacttca ttattatcta ccgactgtcg atgctcgacc tggtcgaaga
    88621 cggcgggact tttgccgaaa cggtagagct ggcgcaggcc attgaagcgg cgggcgcaac
    88681 cattatcaac accggcattg gctggcatga agcacgtatt ccgaccattg ccacgcccgt
    88741 gccgcgcggc gcatttagct gggtcacgcg caaactgaaa ggccacgttt cgctgccgct
    88801 ggtgaccacc aaccgaatta acgatccgca ggttgccgac gacattctct cgcgcggcga
    88861 tgccgatatg gtgtcgatgg cacgaccgtt tcttgctgat gcggagctgc tgtcaaaagc
    88921 gcaatcaggg cgagccgatg agatcaacac ctgcattggc tgcaatcagg cctgcctcga
    88981 tcaaatcttc gttggcaaag tcacctcgtg cctggtgaat cctcgtgcct gccatgaaac
    89041 caaaatgccg atccttcccg ccatgcagaa aaaaaatctg gcggtggtcg gtgcgggacc
    89101 agctgggctg gcgtttgcca ttaacgcggc ggcgcgtggg catcaggtaa cattgtttga
    89161 tgcccacagc gagatcggcg ggcagtttaa tatcgccaaa cagatccccg gcaaagagga
    89221 gttttacgaa acgctgcgct actaccggcg gatgatcgaa gtaacgggcg tgacgctgaa
    89281 actcaatcac accgtaacag cgaatcagtt acaggaattc gatgaaacga tcctcgccag
    89341 tgggatcgtg ccgcgcactc cgcccatcga cgggatcgat catccgaagg tattgagtta
    89401 tctcgatgta ctgcgcgaca aagcgccggt gggcaacaaa gttgccatca tcggttgtgg
    89461 cgggattggt tttgatacgg cgatgtattt aagtcagccg ggcgaatcca ccagccagaa
    89521 tatcgctgag ttttgtaatg agtgggggat cgacagtagc ctacaacagg ctggtggctt
    89581 aagcccgcag ggaatgcaga tcccgcgtag cccacggcag attgtgatgc tccagcgcaa
    89641 agccagcaaa ccgggacagg ggttaggcaa aaccaccggc tggatccatc gcaccaccct
    89701 gctctcgcgg ggtgtgaaaa tgatcccagg cgtaagttat cagaagattg acgatgacgg
    89761 gctgcatgtg gtgatcaacg gcgaaacgca ggtattagca gtggacaatg tggtgatctg
    89821 cgcagggcaa gagccaaacc gcgcgctggc gcagccgctg attgatagcg ggaaaacggt
    89881 gcatttaatt ggcggctgcg atgtggctat ggagctggac gcacgacggg caattgccca
    89941 gggaacacgg ctggcgctgg aga
//
LOCUS       DAZGCQ010000004        84556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000004 DAZGCQ010000000
VERSION     DAZGCQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.004"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(482..1096)
                     /gene="yfdX"
                     /locus_tag="ACHK07_000282"
     CDS             complement(482..1096)
                     /gene="yfdX"
                     /locus_tag="ACHK07_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO8100676.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQKTAAAERISE
                     QGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNVNDDQ
                     YIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMP
                     LKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1392..1667
                     /gene="ypdI"
                     /locus_tag="ACHK07_000283"
     CDS             1392..1667
                     /gene="ypdI"
                     /locus_tag="ACHK07_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO8100677.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKASYLIHPVR"
     gene            complement(1744..1986)
                     /gene="yfdY"
                     /locus_tag="ACHK07_000284"
     CDS             complement(1744..1986)
                     /gene="yfdY"
                     /locus_tag="ACHK07_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO8100678.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2339..3259
                     /gene="lpxP"
                     /locus_tag="ACHK07_000285"
     CDS             2339..3259
                     /gene="lpxP"
                     /locus_tag="ACHK07_000285"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO8100679.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3615..3686
                     /gene="ypdK"
                     /locus_tag="ACHK07_000286"
     CDS             3615..3686
                     /gene="ypdK"
                     /locus_tag="ACHK07_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO8100680.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3751..4989)
                     /gene="alaC"
                     /locus_tag="ACHK07_000287"
     CDS             complement(3751..4989)
                     /gene="alaC"
                     /locus_tag="ACHK07_000287"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO8100681.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVSGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5366..7063
                     /gene="ypdA"
                     /locus_tag="ACHK07_000288"
     CDS             5366..7063
                     /gene="ypdA"
                     /locus_tag="ACHK07_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO8100682.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            7078..7812
                     /gene="ypdB"
                     /locus_tag="ACHK07_000289"
     CDS             7078..7812
                     /gene="ypdB"
                     /locus_tag="ACHK07_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO8100683.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            7825..8682
                     /gene="ypdC"
                     /locus_tag="ACHK07_000290"
     CDS             7825..8682
                     /gene="ypdC"
                     /locus_tag="ACHK07_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8100684.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRENVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(8685..11180)
                     /gene="ptsP"
                     /locus_tag="ACHK07_000291"
     CDS             complement(8685..11180)
                     /gene="ptsP"
                     /locus_tag="ACHK07_000291"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8100685.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGEIRYLPLL
                     LGLDLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVIGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(11205..12242)
                     /gene="ypdE"
                     /locus_tag="ACHK07_000292"
     CDS             complement(11205..12242)
                     /gene="ypdE"
                     /locus_tag="ACHK07_000292"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8100686.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(12242..13327)
                     /gene="ypdF"
                     /locus_tag="ACHK07_000293"
     CDS             complement(12242..13327)
                     /gene="ypdF"
                     /locus_tag="ACHK07_000293"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8100687.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSTESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(13342..14589)
                     /gene="fryC"
                     /locus_tag="ACHK07_000294"
     CDS             complement(13342..14589)
                     /gene="fryC"
                     /locus_tag="ACHK07_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8100688.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(14611..14937)
                     /gene="fryB"
                     /locus_tag="ACHK07_000295"
     CDS             complement(14611..14937)
                     /gene="fryB"
                     /locus_tag="ACHK07_000295"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8100689.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(15156..16121)
                     /gene="glk"
                     /locus_tag="ACHK07_000296"
     CDS             complement(15156..16121)
                     /gene="glk"
                     /locus_tag="ACHK07_000296"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO8100690.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            16325..17582
                     /gene="yfeO"
                     /locus_tag="ACHK07_000297"
                     /pseudo
     CDS             16325..17582
                     /gene="yfeO"
                     /locus_tag="ACHK07_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
     gene            17697..18023
                     /gene="ypeC"
                     /locus_tag="ACHK07_000298"
     CDS             17697..18023
                     /gene="ypeC"
                     /locus_tag="ACHK07_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO8100691.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(18164..19402)
                     /gene="mntH"
                     /locus_tag="ACHK07_000299"
     CDS             complement(18164..19402)
                     /gene="mntH"
                     /locus_tag="ACHK07_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO8100692.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            19738..20940
                     /gene="nupC"
                     /locus_tag="ACHK07_000300"
     CDS             19738..20940
                     /gene="nupC"
                     /locus_tag="ACHK07_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO8100693.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(20990..23179)
                     /gene="pdeA"
                     /locus_tag="ACHK07_000301"
     CDS             complement(20990..23179)
                     /gene="pdeA"
                     /locus_tag="ACHK07_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8100694.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(23387..23462)
                     /locus_tag="ACHK07_000302"
     tRNA            complement(23387..23462)
                     /locus_tag="ACHK07_000302"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23427..23429),aa:Ala,seq:ggc)
     gene            complement(23502..23577)
                     /locus_tag="ACHK07_000303"
     tRNA            complement(23502..23577)
                     /locus_tag="ACHK07_000303"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23542..23544),aa:Ala,seq:ggc)
     gene            23798..24157
                     /gene="yfeC"
                     /locus_tag="ACHK07_000304"
     CDS             23798..24157
                     /gene="yfeC"
                     /locus_tag="ACHK07_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8100695.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            24159..24551
                     /gene="yfeD"
                     /locus_tag="ACHK07_000305"
     CDS             24159..24551
                     /gene="yfeD"
                     /locus_tag="ACHK07_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8100696.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(24603..26018)
                     /gene="gltX"
                     /locus_tag="ACHK07_000306"
     CDS             complement(24603..26018)
                     /gene="gltX"
                     /locus_tag="ACHK07_000306"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO8100697.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            26277..26352
                     /locus_tag="ACHK07_000307"
     tRNA            26277..26352
                     /locus_tag="ACHK07_000307"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26310..26312,aa:Val,seq:tac)
     gene            26397..26472
                     /locus_tag="ACHK07_000308"
     tRNA            26397..26472
                     /locus_tag="ACHK07_000308"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26430..26432,aa:Val,seq:tac)
     gene            26519..26594
                     /locus_tag="ACHK07_000309"
     tRNA            26519..26594
                     /locus_tag="ACHK07_000309"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26552..26554,aa:Val,seq:tac)
     gene            26599..26674
                     /locus_tag="ACHK07_000310"
     tRNA            26599..26674
                     /locus_tag="ACHK07_000310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26632..26634,aa:Lys,seq:ttt)
     gene            26705..26830
                     /locus_tag="ACHK07_000311"
     ncRNA           26705..26830
                     /ncRNA_class="other"
                     /locus_tag="ACHK07_000311"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            26950..27291
                     /locus_tag="ACHK07_000312"
     CDS             26950..27291
                     /locus_tag="ACHK07_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO8100698.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(27282..28208)
                     /gene="yfeR"
                     /locus_tag="ACHK07_000313"
     CDS             complement(27282..28208)
                     /gene="yfeR"
                     /locus_tag="ACHK07_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8100699.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            28298..29296
                     /gene="yfeH"
                     /locus_tag="ACHK07_000314"
     CDS             28298..29296
                     /gene="yfeH"
                     /locus_tag="ACHK07_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO8100700.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(29293..29511)
                     /gene="ypeB"
                     /locus_tag="ACHK07_000315"
     CDS             complement(29293..29511)
                     /gene="ypeB"
                     /locus_tag="ACHK07_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO8100701.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(29513..31528)
                     /gene="ligA"
                     /locus_tag="ACHK07_000316"
     CDS             complement(29513..31528)
                     /gene="ligA"
                     /locus_tag="ACHK07_000316"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO8100702.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(31599..32597)
                     /gene="zipA"
                     /locus_tag="ACHK07_000317"
     CDS             complement(31599..32597)
                     /gene="zipA"
                     /locus_tag="ACHK07_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO8100703.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32827..33588
                     /gene="cysZ"
                     /locus_tag="ACHK07_000318"
     CDS             32827..33588
                     /gene="cysZ"
                     /locus_tag="ACHK07_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO8100704.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            33773..34744
                     /gene="cysK"
                     /locus_tag="ACHK07_000319"
     CDS             33773..34744
                     /gene="cysK"
                     /locus_tag="ACHK07_000319"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO8100705.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDIFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            35128..35385
                     /gene="ptsH"
                     /locus_tag="ACHK07_000320"
     CDS             35128..35385
                     /gene="ptsH"
                     /locus_tag="ACHK07_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO8100706.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            35430..37157
                     /gene="ptsI"
                     /locus_tag="ACHK07_000321"
     CDS             35430..37157
                     /gene="ptsI"
                     /locus_tag="ACHK07_000321"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO8100707.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            37198..37707
                     /gene="crr"
                     /locus_tag="ACHK07_000322"
     CDS             37198..37707
                     /gene="crr"
                     /locus_tag="ACHK07_000322"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO8100708.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(37750..38601)
                     /gene="pdxK"
                     /locus_tag="ACHK07_000323"
                     /gene_synonym="thiJ"
     CDS             complement(37750..38601)
                     /gene="pdxK"
                     /locus_tag="ACHK07_000323"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO8100709.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLVEA"
     gene            38706..39074
                     /gene="yfeK"
                     /locus_tag="ACHK07_000324"
     CDS             38706..39074
                     /gene="yfeK"
                     /locus_tag="ACHK07_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO8100710.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(39077..39988)
                     /gene="cysM"
                     /locus_tag="ACHK07_000325"
     CDS             complement(39077..39988)
                     /gene="cysM"
                     /locus_tag="ACHK07_000325"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO8100711.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQRAGI"
     gene            complement(40108..41205)
                     /gene="cysA"
                     /locus_tag="ACHK07_000326"
     CDS             complement(40108..41205)
                     /gene="cysA"
                     /locus_tag="ACHK07_000326"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO8100712.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDNAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(41195..42070)
                     /gene="cysW"
                     /locus_tag="ACHK07_000327"
     CDS             complement(41195..42070)
                     /gene="cysW"
                     /locus_tag="ACHK07_000327"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO8100713.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(42070..42903)
                     /gene="cysT"
                     /locus_tag="ACHK07_000328"
     CDS             complement(42070..42903)
                     /gene="cysT"
                     /locus_tag="ACHK07_000328"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO8100714.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(42903..43919)
                     /gene="cysP"
                     /locus_tag="ACHK07_000329"
     CDS             complement(42903..43919)
                     /gene="cysP"
                     /locus_tag="ACHK07_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO8100715.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(44223..45014)
                     /gene="ucpA"
                     /locus_tag="ACHK07_000330"
     CDS             complement(44223..45014)
                     /gene="ucpA"
                     /locus_tag="ACHK07_000330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO8100716.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPVSVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(45143..46000)
                     /gene="murR"
                     /locus_tag="ACHK07_000331"
     CDS             complement(45143..46000)
                     /gene="murR"
                     /locus_tag="ACHK07_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO8100717.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKQLALEQTCALFDFARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            46164..47060
                     /gene="murQ"
                     /locus_tag="ACHK07_000332"
     CDS             46164..47060
                     /gene="murQ"
                     /locus_tag="ACHK07_000332"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO8100718.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRKVLDKE"
     gene            47064..48488
                     /gene="murP"
                     /locus_tag="ACHK07_000333"
     CDS             47064..48488
                     /gene="murP"
                     /locus_tag="ACHK07_000333"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO8100719.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSSQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            48493..49797
                     /gene="pbp4b"
                     /locus_tag="ACHK07_000334"
     CDS             48493..49797
                     /gene="pbp4b"
                     /locus_tag="ACHK07_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO8100720.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(49855..50754)
                     /gene="yfeX"
                     /locus_tag="ACHK07_000335"
     CDS             complement(49855..50754)
                     /gene="yfeX"
                     /locus_tag="ACHK07_000335"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO8100721.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(50850..51425)
                     /gene="yfeY"
                     /locus_tag="ACHK07_000336"
     CDS             complement(50850..51425)
                     /gene="yfeY"
                     /locus_tag="ACHK07_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO8100722.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(51486..51935)
                     /gene="yfeZ"
                     /locus_tag="ACHK07_000337"
     CDS             complement(51486..51935)
                     /gene="yfeZ"
                     /locus_tag="ACHK07_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO8100723.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(51922..52347)
                     /gene="ypeA"
                     /locus_tag="ACHK07_000338"
     CDS             complement(51922..52347)
                     /gene="ypeA"
                     /locus_tag="ACHK07_000338"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO8100724.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            52561..53430
                     /gene="amiA"
                     /locus_tag="ACHK07_000339"
     CDS             52561..53430
                     /gene="amiA"
                     /locus_tag="ACHK07_000339"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO8100725.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            53434..54333
                     /gene="hemF"
                     /locus_tag="ACHK07_000340"
     CDS             53434..54333
                     /gene="hemF"
                     /locus_tag="ACHK07_000340"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO8100726.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRKEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54339..55391)
                     /gene="eutR"
                     /locus_tag="ACHK07_000341"
     CDS             complement(54339..55391)
                     /gene="eutR"
                     /locus_tag="ACHK07_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO8100727.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(55437..55937)
                     /gene="eutK"
                     /locus_tag="ACHK07_000342"
     CDS             complement(55437..55937)
                     /gene="eutK"
                     /locus_tag="ACHK07_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO8100728.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(55950..56609)
                     /gene="eutL"
                     /locus_tag="ACHK07_000343"
     CDS             complement(55950..56609)
                     /gene="eutL"
                     /locus_tag="ACHK07_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO8100729.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(56619..57506)
                     /gene="eutC"
                     /locus_tag="ACHK07_000344"
     CDS             complement(56619..57506)
                     /gene="eutC"
                     /locus_tag="ACHK07_000344"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO8100730.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(57527..58888)
                     /gene="eutB"
                     /locus_tag="ACHK07_000345"
     CDS             complement(57527..58888)
                     /gene="eutB"
                     /locus_tag="ACHK07_000345"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO8100731.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(58900..60303)
                     /gene="eutA"
                     /locus_tag="ACHK07_000346"
     CDS             complement(58900..60303)
                     /gene="eutA"
                     /locus_tag="ACHK07_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO8100732.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVCAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(60300..61526)
                     /gene="eutH"
                     /locus_tag="ACHK07_000347"
     CDS             complement(60300..61526)
                     /gene="eutH"
                     /locus_tag="ACHK07_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO8100733.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHILGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(61743..62930)
                     /gene="eutG"
                     /locus_tag="ACHK07_000348"
     CDS             complement(61743..62930)
                     /gene="eutG"
                     /locus_tag="ACHK07_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO8100734.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVCLYA
                     AAQ"
     gene            complement(62920..63756)
                     /gene="eutJ"
                     /locus_tag="ACHK07_000349"
     CDS             complement(62920..63756)
                     /gene="eutJ"
                     /locus_tag="ACHK07_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO8100735.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRIPLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(63767..65170)
                     /gene="eutE"
                     /locus_tag="ACHK07_000350"
     CDS             complement(63767..65170)
                     /gene="eutE"
                     /locus_tag="ACHK07_000350"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO8100736.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(65182..65469)
                     /gene="eutN"
                     /locus_tag="ACHK07_000351"
     CDS             complement(65182..65469)
                     /gene="eutN"
                     /locus_tag="ACHK07_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO8100737.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(65576..65869)
                     /gene="eutM"
                     /locus_tag="ACHK07_000352"
     CDS             complement(65576..65869)
                     /gene="eutM"
                     /locus_tag="ACHK07_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO8100738.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(65908..66924)
                     /gene="pta"
                     /locus_tag="ACHK07_000353"
     CDS             complement(65908..66924)
                     /gene="pta"
                     /locus_tag="ACHK07_000353"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8100739.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGIADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(66921..67724)
                     /gene="eutT"
                     /locus_tag="ACHK07_000354"
     CDS             complement(66921..67724)
                     /gene="eutT"
                     /locus_tag="ACHK07_000354"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO8100740.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(67721..68422)
                     /gene="eutQ"
                     /locus_tag="ACHK07_000355"
     CDS             complement(67721..68422)
                     /gene="eutQ"
                     /locus_tag="ACHK07_000355"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO8100741.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHAGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(68397..68876)
                     /gene="eutP"
                     /locus_tag="ACHK07_000356"
     CDS             complement(68397..68876)
                     /gene="eutP"
                     /locus_tag="ACHK07_000356"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO8100742.1"
                     /translation="MKRIAFVGTVGAGKTTLFNVLQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(68889..69224)
                     /gene="eutS"
                     /locus_tag="ACHK07_000357"
     CDS             complement(68889..69224)
                     /gene="eutS"
                     /locus_tag="ACHK07_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO8100743.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(69517..71796)
                     /gene="maeB"
                     /locus_tag="ACHK07_000358"
     CDS             complement(69517..71796)
                     /gene="maeB"
                     /locus_tag="ACHK07_000358"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO8100744.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            72085..73035
                     /gene="tal"
                     /locus_tag="ACHK07_000359"
     CDS             72085..73035
                     /gene="tal"
                     /locus_tag="ACHK07_000359"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8100745.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            73055..75058
                     /gene="tkt"
                     /locus_tag="ACHK07_000360"
     CDS             73055..75058
                     /gene="tkt"
                     /locus_tag="ACHK07_000360"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8100746.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(75153..76196)
                     /gene="ypfG"
                     /locus_tag="ACHK07_000361"
     CDS             complement(75153..76196)
                     /gene="ypfG"
                     /locus_tag="ACHK07_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO8100747.1"
                     /translation="MRYRNLLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(76322..76897)
                     /gene="nudK"
                     /locus_tag="ACHK07_000362"
     CDS             complement(76322..76897)
                     /gene="nudK"
                     /locus_tag="ACHK07_000362"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO8100748.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(76965..78944)
                     /gene="aegA"
                     /locus_tag="ACHK07_000363"
     CDS             complement(76965..78944)
                     /gene="aegA"
                     /locus_tag="ACHK07_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO8100749.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            79150..80850
                     /gene="narQ"
                     /locus_tag="ACHK07_000364"
     CDS             79150..80850
                     /gene="narQ"
                     /locus_tag="ACHK07_000364"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO8100750.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            81014..84127
                     /gene="acrD"
                     /locus_tag="ACHK07_000365"
     CDS             81014..84127
                     /gene="acrD"
                     /locus_tag="ACHK07_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO8100751.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(84226..84285)
                     /gene="ypfM"
                     /locus_tag="ACHK07_000366"
     CDS             complement(84226..84285)
                     /gene="ypfM"
                     /locus_tag="ACHK07_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO8100752.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        20110 a        22968 c        21394 g        20084 t
ORIGIN      
        1 ttaatttagc gtttatttaa ggcataacgc ctctataaat aatccgcaaa atatcgtcgt
       61 agcggcactc gatattttta cattcctgta tcaagtgctg aaaatcattt catcgctact
      121 tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattgg
      181 ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc
      241 gccggtgatt tacaagtaag ggtatgatta cgctttttaa agcgtaccat aaaatataac
      301 atattaaatg atttgtgatg acacgggatt attgcggttt actttgagga atataaaatg
      361 cgagtttata gaacagcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt
      421 cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc
      481 attagttcac aaacagtgct tcgctatcga cgatgattcc atcttccgca cctttaagcg
      541 ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat
      601 tacgagtctg tttaagcggc atcagatatt ggttttccat tacaccaaca ccggccaggc
      661 gaagctcttc cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta
      721 tcgcggcttc tttttcgggt gtcgctacat aactctcaga aattccgaca gatgcattaa
      781 tgacaatata ttggtcatca ttgacattgg tctttttacc tggcttagcg aactttgccc
      841 attcggtact gtcatctgac aacaaagctg aagcttcatt ggtcagttct tttgcctttt
      901 ccggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac
      961 cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgttgt gccgctaccg
     1021 gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca
     1081 taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata
     1141 ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac
     1201 agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat atgcttcctg
     1261 tcacagagaa ctattcttat gtaccacaaa gagatgtcat ttattatatc gatgtaaata
     1321 ttgattaatt atatgtaaat acagccataa catcgtacat tctcagtgaa ccattttcag
     1381 gataactttc tatgaaagta aacttaatac tttttagctt atttttattg gtctctatta
     1441 tggcatgcaa tgtttttgca ttttctaatt ctggtggtgg tagtgagacg agctataaag
     1501 agccagaaaa aacgtcagcg atgacgacaa ctcactctac aaaatttcag ccatcacagg
     1561 cgattttgtt taagatgaga gaagatgcac caccattaaa cctcacagaa gaaaccacgc
     1621 ccccttttcc gacaaaggcg agttatctta ttcatcctgt gcgatagcgt ctgtcacatg
     1681 gcaggtgagc aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac
     1741 ccactaccgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag
     1801 aaacataaat atccccgtca ccagttcatt accgccagtt aaccacatgg tgaagtaata
     1861 aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag
     1921 tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata
     1981 aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta
     2041 agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg
     2101 caacactatt gcatgcggaa aattttttac tttgcacgat taataatcag tagctgaaag
     2161 cagtcagcca gtgataaagt agagccaccg cattcatgct cctcgtggtt atgtcctgac
     2221 tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt
     2281 accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat
     2341 gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt
     2401 tggacttggt gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg
     2461 cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgg cgtgaatcta tcgcccgtaa
     2521 aaacctggaa ctttgcttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga
     2581 aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcgtggt tctggcccga
     2641 cagtcgcgta cgtaaatggt ttgatgttga agggctggat aaccttaaac gcgcacaaat
     2701 gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg
     2761 cgtgatggga ctttgtcagc caatgatggc tacctatcgt ccacataata atcagctgat
     2821 ggaatgggtg cagacccgtg gtcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa
     2881 tctgcgcggc attgtcagtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca
     2941 ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtagaaa atgtcgccac
     3001 aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat
     3061 ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta
     3121 cccgacagat gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagaaatcat
     3181 gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga
     3241 atcgtcgtta tacatttaac aaactgctct gactctttac ctcctcacat aagccataat
     3301 aacctcatgg tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta
     3361 ttaccgtcag aaattaaact gtcgccgata tgagacacct gtaagttact aaaagtaaat
     3421 aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt
     3481 ttagctcaga attctggggt gatgcccacg gtaaaataca ggacttttaa ggtcgttttg
     3541 cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agcaattacg
     3601 cggagagatg taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg
     3661 ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca
     3721 ggctcctgtt tgcatttctg aggtcggtgt ttattccgcg ttttcgtgaa tatgtttgct
     3781 gctggcgggt agtaaaccgt cggcacggaa catcgcttta atcccacgaa tcgcctgacg
     3841 aatacggtcg cggttttcaa tcagtgcaaa gcgcacatgc gtatcaccat agtcgccaaa
     3901 gccaatcccc ggagagacac agaccttcgc ttcgttaagc aatttcttgg caaattccag
     3961 cgagcccatg gcagcatacg gttccggtat tttcgcccag acatacatcg aagccttcgg
     4021 catttcgacc atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt
     4081 gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tccagcgccg caatcgccgc
     4141 cacctgcaac ggcgtaaagg tgccgtaatc gtgatagctt ttaatacgtg ccagcgcact
     4201 gaccagcgtt ttgttgccaa ccataaagcc gatacgccag cccgccatgt tgtagctttt
     4261 cgacagcgta aagaactcga ccgccacatc gcgtgcacct gacacctgca tgattgacgg
     4321 cgctttccag ccatcgtaga cgatatcggc ataggccagg tcatggacca ccagcacatc
     4381 gtagcgcttc gccagcgcca caaccttttc gaagaactcc agttccacgc attgcgcggt
     4441 tgggttcgac gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc
     4501 gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgagc
     4561 cccggcaatc accgcgccgt aaatatgaat ggggtaactt ggattcggca ccagcactgt
     4621 gtcaccgtga tccagtgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt
     4681 gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga
     4741 aatggcgcga cgtagccgcg gaataccgcg tgaagtggag taaccatgcg tgtccgggcg
     4801 ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc
     4861 catactgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt
     4921 aatgttaaaa acatagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt
     4981 gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac
     5041 gatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat
     5101 ttttattaat gacgttaaat gaggaactct gctttaaaaa caggaaaatt atctgcgcta
     5161 acaagaaaat taccactcat tttattaaca taataatttc aataaattta ctaaccagaa
     5221 gtgcattatc aagagatgca ttgctaatac accatttatc tatcaatccc cttttaaaat
     5281 tgtggttttt cctcaaaaac ggcctgctgg tcatcagcct tcaggttacc tatcatagag
     5341 gtttaatcct tattcagagt cacccgtgca cgaaatattc aacatgctgc tggcggtctt
     5401 cgatcgggca gcgttaatgc ttatctgcct gttctttctc atccgtatcc gcctgtttcg
     5461 cgaactgttg cacaagtcgg cacactcacc gaaagagttg ctcgccgtta ccgccatttt
     5521 ctcgctgttc gccctcttca gcacctggtc cggcgttccc gtagaaggct cgctggtgaa
     5581 cgtacgtatt atcgcggtga tgtccggcgg gattctgttt ggcccgtggg tgggcatcat
     5641 taccggcgtg attgcgggta ttcatcgtta tttaattgat atcggtggcg tgacggcgat
     5701 cccctgcttt atcaccagca ttctggctgg ttgtatatcg ggctggatca acctgaaaat
     5761 ccccaaagca cagcgctggc gcgtcggtat tctcggcggc atgttgtgtg aaacgttgac
     5821 catgattctg gtgattgtct gggcaccaac taccgcgttg gggatcgata tcgtctctaa
     5881 aatcgggatt ccaatgatcc tcggcagcgt ctgtatcggc tttattgtgc ttctggtgca
     5941 aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag gccaagttgg cgctggatat
     6001 cgccaacaaa acgctacctc tgtttcgcca tgtcaatagc gagtcattac gcaaagtttg
     6061 tgaaattatt cgcgatgaca ttcacgccga tgcggtggcg attaccaata ccgatcatgt
     6121 gctggcctat gttggtgtgg gtgaacataa ctatcagaat ggcgatgact tcattagccc
     6181 gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg atgaagccca
     6241 ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag gcgttgtgac
     6301 cggaacgctg aaaatttact actgccacgc gcatcagata acctcgtcat tacaggaaat
     6361 ggcggttggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg ccgagcagct
     6421 acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta atccccattt
     6481 tctgtttaac gctctgaacg ctatttcatc gtcgatccgt ctgaatccgg ataccgctcg
     6541 ccagctgatc tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga
     6601 gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc
     6661 ccgctttggc gacaagctca cggttatcta tgatattgat gaagaggtga attgctgcat
     6721 tcccagcctg ctgatccagc cgttggtgga gaatgccatt gtccacggta tccagccttg
     6781 caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggcaatcggg tacgcattgc
     6841 cgtgcgggat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg aagcgaatga
     6901 aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg
     6961 cgaggggctg catatccgcc gcctggaacc aggaacggaa attgcgtttt acattcctaa
     7021 ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg
     7081 aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa
     7141 gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt
     7201 ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggt
     7261 gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc
     7321 gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa
     7381 ccgtatcagg agtcacgcat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa
     7441 cagcagacca gcagcacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg
     7501 gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac
     7561 gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc
     7621 gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc
     7681 aatctgaaca aaatccgcga gatcgaaccg tggtttaata acacctatat tctgcgactg
     7741 aaagatctgg attttgaagt gccagtcagc cgcagcaaag taaaagaatt tcgccagtta
     7801 atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt
     7861 ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg cgtctggttt
     7921 gccagccagc ctgcctcgtt gccggtgggc agtttatgta ttgattttcc ccgtctggat
     7981 atcgtgctgc gcggcgaata cggcaatctg ctggaagcaa agcagcaacg tttggtggaa
     8041 ggagaaatgc tgtttattcc ggcgcgcgcg gctaatttac cggtcaacaa caaaccggtg
     8101 atgctgttaa gcctggtgtt cgctccgacc tggcttgggt tatcgtttta cgatagccgc
     8161 accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca acgcggtgaa
     8221 ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcaaaatatc
     8281 attcagccgc tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg
     8341 ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggttcaggat
     8401 aattatgccc agccgctcac ccgtgagaac gtggcgcagt tttttaatat cacgcccaat
     8461 catctgtcaa aactgtttgc gcagcatgga acaatgcgtt ttatcgaata tgtgcgttgg
     8521 gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcgat tcatgaagtg
     8581 gcgcagcgtt gcggctttcc ggacagcgac tatttttgtc gcgtcttccg gcgtcagttt
     8641 ggcctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct
     8701 gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag
     8761 tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg
     8821 ttagcgccca gcgtcagcat gattaccagt tcgacttcgc ccatctctga ctgccagtca
     8881 accggcttca ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg
     8941 tgcgggatcg ccacgccaaa accaacgccg gtggtgacaa tctcttcccg ctgccagaca
     9001 tcctcttcca gctcgaacgg atgttcagtg cgcccgataa cgccgaggtt accgcacagg
     9061 aactggatcg cctgctcttt attgctaaag gcctgatcaa cgaagatatt ctccagcgcc
     9121 aacaatgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc gatttcctgc
     9181 gcgctgcgac attcacacgc ctgacgtgcc agttcccgac acgcctcgct atccagttga
     9241 cgcagctggc ttttcaccgc cggaatgcgc gggctactca tactcagctc atccaggtct
     9301 agcccaagca gtagcggcag ataacggatt tcaccgccca gttcaccgca aatgcctacc
     9361 catttgcccc gctgatgcgc agtggtaaca atctgctgca acatgcgcag gaacgatggc
     9421 gtaatcgggt tataaagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac
     9481 tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa gtgatcgatg
     9541 atgtagcaca ccgacggcac ttccaccatg atacccagcg taatcgtctc tgcatgacgc
     9601 aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcccttcac ccataagatc
     9661 tgatcgaggc tgtgaaccat cgggatcatc aactgggcgt tgccgaaact ggcggcgcgc
     9721 aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg
     9781 cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg
     9841 ccgccgatgt ccattgtgcg gaagataatc ggcttgtcgc ccgccgccag tagcacctgc
     9901 tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt
     9961 tcggtacgga acagcccgac gccttccgcg ccgttggcaa acgcgccagg cgcttccaga
    10021 gcggtgccaa tattcgccgc gatatcaata cgcttgttat cacgggaata agccagctgc
    10081 gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa
    10141 taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact
    10201 gcaggttgcc cggcataacg ggcaatcgca tccagcggca ggccgctcag taccgggatc
    10261 gccgaggcgc gggccagaat cagggtatgc gaggtgcgcc cggttttctc cagaatcatg
    10321 cccgcaagat tttttaaatc caggcttaaa aactggcttg gggttaaatc ctcggccacc
    10381 agaatagtcg gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtaata
    10441 tgcagcaact gttcgctaat atcgcgaatg tcgctaacac gttcgcgcag ataatcgctg
    10501 gcagaagcag aaagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagc
    10561 ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc
    10621 agcgacaaat gggcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctgg
    10681 ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg
    10741 gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg
    10801 ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa
    10861 cgcggcagcg gatgcgccgt cagttctgcc tgcgtaggct gaacgctgtc gctgtcgata
    10921 aagcgcacct ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta
    10981 atgttcaggc tgcaactgtc attaaatagg gtgccggtgc caatcagcgc cagcgagctt
    11041 ttggcatcgg cctttgcgtt ctggcgatgg ttaataaaag tgatttcgct ttgccactgg
    11101 ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag
    11161 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
    11221 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
    11281 tgcaaaatgt cgcggcaatc ggcaatcgat gcggcgcaat gtccgtggcg ggtggcaggc
    11341 cccatcacca ctgtgggtac gccggtaccg gttaagtgca ccgctccacc gtcagtgccg
    11401 ccgttactga acatatccgc ctgtaacggc acgccaattt ctgccgccac ggtttcgatc
    11461 caggcggtga gttttggcgg cgcaatcagt gacttgtcgc ttaacaccag catcgggccg
    11521 ttaccaatct ggcgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
    11581 tcaaggacaa tggcgacgtc cggcgacacc gcgcgggtgg cagtttgccc gccgcgtaat
    11641 cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc
    11701 agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa ggctttcccc
    11761 atcacccgct ggtgggggag aacctgaaaa gtggtatcga acgtgacgcg atcgcctgga
    11821 cgaattcccg cctgcatcac ttcgtcatac gagcgcgcgc caatatccac gcgcatggcg
    11881 ctgacatcat tcccctgccg gtcgccatca agcagaccgg gaattttgca ctcttcacgg
    11941 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gtacgttgcc aaccggcagt
    12001 acatcaatcg ccccttcgcg ggagatgctg cgcaccataa atcccacttc atccatatgc
    12061 gcacagatca tcaccttcgg acctgtcgat tcattcaggc ggatcagcac cgatcccaga
    12121 ccatcaaatc gcacttcttt ttgcaggcga tccgcttctt ccagcaggat ctgccgtact
    12181 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
    12241 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
    12301 gcggggtgac cagcacaaca tcctcgatgc gcacgccccc ttgccctggc aaataaatcc
    12361 ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc ggtgaaaagc
    12421 gcggatcttc gtgtacttca atgccaatag catgaccggt gttgtgacca aaatagtcgc
    12481 caaatcctgc ctcggtaatc acccgacgcg cggcttcgtc aacctgctgg cagcgcacac
    12541 cggggcgaat tgcagagatt gctgcgagct gtgcctgcaa aacaatctga tagacgttaa
    12601 acagcggatg agattcggtg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat
    12661 cagagcagta gccctgatac agcgcaccga aatcgagagt gacaaactcg cccgctgcaa
    12721 caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccactg gcaacaatgg
    12781 tgtcaaaaga ggttttttct gcgccctgct ggcgcataaa ccactccagt tcagcggcta
    12841 tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctctgca ccgcgatcgg
    12901 caatcccaca ggcaaggcgg attttctcca cctcctctgg cgttttgatt tgccgcagca
    12961 catccggcgt ggcgctaacc agtttcgcat tgagttcaga ctgccagcgg tgcgcggttt
    13021 cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgttcatcg gcaatgattt
    13081 cattgacgat ggttgcaagc gtgtgtgtcg cgtcaagcaa atgcagctgg taaccttgcg
    13141 tacgagcttc tacatccgcg taatagcgcg agtccaccag aatgtgcgca ctttcacggc
    13201 taatcagcac atagcctgat ccggtggaga tccccagatg cggctgtttg ttctgccgtg
    13261 aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc gaagcgagta
    13321 atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt tacggaacat
    13381 catcagacgc aggaacacca ccatcagcgc agtaatgact gcccccagcg cgatccccgc
    13441 catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt ccgggaacca
    13501 ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga ccatatacga
    13561 cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac tgatccccat
    13621 aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgtgcgttga acaggcgttt
    13681 gccggttaaa cgacggtcaa taatggtcgc cagccccaga ccaatcggcg gaataacgat
    13741 agcgatagaa cgtgcggtga ccggtaatac gtggtcagtg gtaaagctga aggcaacgaa
    13801 acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg cgatccccat
    13861 tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acagtacgga taccgccgtt
    13921 gatccagcca ccaaaggggg tgatgacgta gtacatcgcc agcatgacaa aaatagcgga
    13981 aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta actggatttt
    14041 ctggttcatc catttcacaa ggtaaccagc aacgatagaa ataatcaggg caccaatgaa
    14101 ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga agttcagcag
    14161 ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc
    14221 accgatagag ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa acaacacgcc
    14281 gccgaaggac tgcgacaacc aggcaaattt cagtaaggag aggtcgaaac cggagaattt
    14341 cccgctatta agagcatcca tgatgccgat atcagcagga attttaagcc agctataagc
    14401 aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga
    14461 aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg
    14521 attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt
    14581 aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc
    14641 gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga tttcataaac
    14701 gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat ggatgatgat
    14761 ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg cgccctgggt
    14821 ttcaattttc acttcataac cggcttctac cgctgcttct tccagcgcct gagcggccat
    14881 aaaggtgtga gccaggccca tcgggcaggc acataaggca atcagtttct tactcatcgt
    14941 taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg
    15001 ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa gtgtgacgca
    15061 ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt taaaagatta
    15121 tcgggagagt tatctcccga tataacagga aggatttaca gaatgtgccc taaggtctgg
    15181 cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag atacaccgga
    15241 atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg gaaaccagag
    15301 gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac gccgccaaat
    15361 gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca aaacagcgac
    15421 aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat atcttttggc
    15481 ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa attcactagc
    15541 ccagggccag aaagcacgcg ttccgccgaa acatggccaa tttccgcacg cagtatttcg
    15601 aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc
    15661 aggcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc cgttccggca
    15721 ccgtaaaccg caataggctt accttctatc ggctctgcgc caccaaactg aatcagatgc
    15781 tcttttttca gcatcggaat cgccattgat acagcggtaa aatcgttaat aatttccaga
    15841 tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt atggttggtc
    15901 attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc tttcacctcg
    15961 accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta atcaagccct
    16021 gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc aagacgtgcg
    16081 ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact gctccgctaa
    16141 agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca acgccggaaa
    16201 ggcaggctcc ctgtaaatat cgatctgggt cacacaatta ctttatcgtt tcagcaccaa
    16261 ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc ataaggaata
    16321 gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg ccgtggcaat
    16381 tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa
    16441 tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac ccctctggat
    16501 catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt tcagccaggg
    16561 tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccggttc cgccctctgc
    16621 gctacctgga cttatcgtag cattaattct cggtcttgct ggcggcgtca gcctggggcc
    16681 ggaacatccg atcatgaccg tcaatatcgc ccttgcggtt gccattggcg ctcgtctgtt
    16741 accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca tcggcgcgct
    16801 gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg gcagtagtga
    16861 agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg gtgcacttac
    16921 taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg gacagatgga
    16981 aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag cagggatggt
    17041 cgccgtatgg tgcttaccac ggttgcacgc gatgatgcat caaatgaaaa atccggtgct
    17101 cgtgctgggt attggcggat ttattctcgg tattctgggg gttattggtg ggccggtttc
    17161 gctgtttaaa ggactggatg agatgcagca gatggtggca aatcaggctt tcagcaccag
    17221 cgattacttt ttgctggcgg tgattaaact gaccgccctg gtcgttgctg ccgccagtgg
    17281 ctttcgcggt ggacgaattt tcccggcagt gttcgtcggc gtggcattag ggttgatgct
    17341 gcatgagcac gtccccgccg ttccagcggc aataaccgtt tcctgcgcta ttctcggcat
    17401 cgtgctggtg gtaacacgcg atggctggtt aagccttttt atggcggcag tcgttgtacc
    17461 caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc tgttattagc
    17521 aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccccccccac gataacgttt
    17581 agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc
    17641 cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt
    17701 tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaacgcag
    17761 gcgaaatcac cctactgcca tcaatcaaat tacaaattgg cgatcgcgat cattacggta
    17821 attactggga cggtggtcac tggcgcgacc gtgactactg gcatcgcaat tatgaatggc
    17881 gtaaaaaccg ctggtggcgt catgataatg gctaccatcg tggctgggat aagcgtaaag
    17941 catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc
    18001 gcggacatgg acaccgccat taaacacatt cgccaatgga gcacaatgcc tgatgcgacg
    18061 ctagcgcgtc ttatcaggca tacaaaccgg ctctttaacg cgtagggcgg ataaggcgtt
    18121 tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat cccagcgccg
    18181 tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc cagcctgtct
    18241 gttttacacg tttgctgttc accagatcgc ccatcaactt gctgtcactg gtgaaaatca
    18301 gcagtggaac cagcgccagg gcgataccaa aacttaacag tacctgactc ataaccagaa
    18361 tccgtgtcgg atctaatccc atcagaatga caataaatga cggcagcatg gtgactgtac
    18421 gacgcaccca cagcgggata tgaaagcgaa tgaagccctg catcaccacc tgccccgcca
    18481 gtgtccccac caccgttgag gacagtccgg cagcaaccag gcttaatcca aagaccgttg
    18541 ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga tcggcaacac
    18601 cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcgcc agattgacaa
    18661 aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa cgttgttgac
    18721 gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca tgcggcataa
    18781 tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt aaactcggga
    18841 tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac tcgacaatgt
    18901 aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagcggt ttttgcccgc
    18961 gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg ccctgcaaca
    19021 acgaaacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc gccaggtcgg
    19081 ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga cgcggatagt
    19141 gatcgcgaat ctgctccgcc agatttttac cggtggcaat ccctagtttg gcagagagga
    19201 tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc tgatagccga
    19261 agctggcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa ccaatcgccg
    19321 caatgaacgc aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt ccgctgctac
    19381 tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct atgtttcatg
    19441 ctatgccaaa cgagaatgat tatcaaattc atttaaatgg gttgtggtga tttctctgat
    19501 agaccaggat tatgactacg aaaagattga tgacttgaat gttatggtta tgtttaatgt
    19561 tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt atgacagctc
    19621 actatttttg aagcttgtca caggacgtca ttatagtgtg tgtcagatct cgttttcctt
    19681 aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg agcaaatatg
    19741 gaccgcgtcc ttcattttgt actggcactt gccgttgttg cgattctcgc actgctggta
    19801 agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt tatcgaagtg
    19861 ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa aggcttctcc
    19921 gaaatgttcg aaaaactgct cggatttgcc aacgaaggga ctaacttcgt ctttggtagc
    19981 atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat cgtctttatc
    20041 tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat ccgcgcaatt
    20101 ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa cgccgtcagc
    20161 tccctgattc tgggtcagtc tgaaaacttt attgcctata aagatatcct tggcaaaatc
    20221 tcccgtaacc gtatgtacac catggcagca acggcgatgt ccaccgtttc tatgtccatc
    20281 gttggtgcat acatgaccat gctggagccg aaatatgtcg ttgcagcgct ggtactgaat
    20341 atgttcagca cctttatcgt gctgtcgctg atcaaccctt accgtgttga tgccagtgaa
    20401 gaaaacattc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat gctgggtgaa
    20461 tacattctgg caggtttcaa agttgccatt atcgttgctg cgatgctgat cggctttatc
    20521 gccctgatcg ccgcgctgaa cgcactgttt gccaccgtga ctggctggtt tggctacagc
    20581 atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt gatgggtgtt
    20641 ccgtccagcg aagcactgca agtgggcagt atcatggcga ccaaactggt ttctaacgag
    20701 ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg tgctgaaggc
    20761 atcatctctg tgttcctggt ttccttcgct aacttctctt caatcggaat tattgcaggt
    20821 gcagttaaag gcctgaatga agagcaaggt aatgtggttt ctcgcttcgg tctgaaactg
    20881 gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact ggtgctgtaa
    20941 gaccatacat aaaaaagccg gggataattc cccggttttt ttacgcctgt taatcagcta
    21001 atggctgcgg gcgaccaatc aaatacccct gcagatattg caccccgagc ttatgcaata
    21061 acgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac ttcgctttcg
    21121 ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca atatctttca
    21181 caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg
    21241 catagccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc agctgctcga
    21301 tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga
    21361 ccgcctgcgg ggagatgtga taacgcttaa atagacgaat aatccgcccg gcaatattct
    21421 tttgcagcag cgtgagcggc attaaattaa ctgaaaagcg cgggcctttt ttgtcgcaag
    21481 ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa cgcgcgctaa
    21541 gattaaactg agcaataagg ggcagaaact tatccggggt cataatgccg ccgtcatatt
    21601 tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt ggctgggcgt
    21661 agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat agcacctgtt
    21721 tagtggtctg cccggaaacc atctcctctc ggctatccag cgccagcaca tgatgatgtg
    21781 cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg gtttcctgat
    21841 ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta ttgttccagt
    21901 gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc gtttccggcc
    21961 cgctcagcac taacagcagt tcactacccg gcaattgata caacttttcg ttttcctgca
    22021 tcaatggcag cagcgtacgg taaattgatc ggatacagtg aacgcgcatc atcaacccgt
    22081 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct
    22141 cttgctccgg cgcttgttcc aacgcacgaa agttgggcag gagcgttaac ggatcggtca
    22201 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    22261 agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg
    22321 tttccacccc ttgcaaaaag ttctggttgt aattcagcag gcaaagcgtt gaaacagccc
    22381 aggtgagatt taaaaacgga tagcgaagct taccgacccc gagggtaaag atgatgaaga
    22441 aaacgggtac caggtaacct gcaataaagt cgttttcata aggtgtgcac agcagaagta
    22501 gcagcacgct taacgctgcc agccagctta aggtaaatgc gcgtttctct ttgcccaatg
    22561 acggagcgat atccctgcgc cacaatatct gcgcaaagtg gggacttaca atcatgcgag
    22621 tgagatagta gaaaagcatg ttgtaaatca gaacagcggt gaaaaggctt agtaaatcca
    22681 cgaccgtgaa aatggcatcc gcatcgccga aaaaggtgga tatctttagc gggaaatcaa
    22741 agaagctccc cacaagatac atgctgcatt tgatgccgat cggcgtcacc aaaccaagcc
    22801 agaacagacg ttgccagaca tatcggctgg tcaatccata acgccaacgc gtccccagct
    22861 gccagcgtaa tattgcgcag gcacacaaca cgacaaatgt ctggctgccg agcatgaccg
    22921 cagtctgtaa aaaagagaga tgaaagttcc acagattggt gcaaaacatc ccgcataaaa
    22981 cgggaacgac accgcgccag ccaaagataa acaagattga cagcattacg cacagtggca
    23041 tccacgccag aaagatataa ctggaatgga taatggcaag tggcgaaata aaacgggata
    23101 gctgaataag taccacggtg agcgtaaacg ctagtgtgaa tatcttgata ttttttatca
    23161 ggttatgctc cacaaacatg agtacactac tctcactgat cggggttttc aggcacacaa
    23221 ataataagtt tattcgcatt gcgaagcaaa caggggccat cctgttgccg atttttcatc
    23281 caatgatgct ttcaccaggc ggcactaacc cggacgtaga ctgtattaca aaagcggcaa
    23341 aaagcagaga caaaaaaccc ccgctttgca gcgagggttg gaaatttggt ggagctaagc
    23401 gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc
    23461 ccacgatgcg tttacgtacc aaatttgctg ggtgcaaaat ttggtggagc taagcgggat
    23521 cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccgtc
    23581 acgtaaagct tgtcgagttg acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg
    23641 gcaaattgca gcgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca attttggaaa
    23701 accgctcgca ttcagtagtt attcatgtca cggtttcctg taaagtggtg ctataaaatg
    23761 aactactaat agacccacat acattcaggg aattgttatg ttcaaggaga ggatgacgcc
    23821 agatgaactt gccagactga ccggctatag ccgccagacc attaataaat gggtacgcaa
    23881 ggaaggctgg acgacgtcac caaaaccagg cgtccagggt ggcaaagccc gactggttca
    23941 cgtcaatgaa caggttcgtg aatatattcg taatgctgaa cgtccagaag gtcagggaga
    24001 agcgcctgcc ctttctggtg atgcgccgct tgaagtattg ctggtgacac tggcgaaaga
    24061 gatgacgcca gttgaacaaa aacagtttac atccttgctt ctgcgggaag ggattatcgg
    24121 attgttacaa cgcttaggga ttcgcgatag caaataatat gaaaagatta cgcaataaaa
    24181 tgaccaccga agaactggct gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga
    24241 tcagagaaaa aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac
    24301 ttattctggt cgatacgcaa gtttgcgagt ttattcagaa cacgcctgcg ttccataaca
    24361 caccaatgtt gatggaagct gaagagagaa ttgctgaata cgctcctgga gcacgcgccc
    24421 cggcgtatcg acaaatcatc aacgcgatag ataatatgac agacattgag caggaaaaag
    24481 tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct cgcccgtctc gatattgacg
    24541 aatcagcata aataaaaaac ggcaggatat tatctcctgc cgtttatctt tttacacgct
    24601 aattactgct ggttttcgcg ttcagcaata aaggccagcg ctttgttgat acgctcaatg
    24661 ctacgtgtct taccgattgc gtgaacggta acatccagtg ccggagactg ccccgcaccg
    24721 gttacggcga cacgcagcgg cataccaact ttacccatac ccacttccag ctcatccgcc
    24781 gtcgcctgaa tagcgtgatg aacgttttca gctgtccagt cagtaatcgc ggccagtttg
    24841 tcacgaacta cttccagcgg ctgacgcgct accggacgca gatgtttttt cgcggcatcg
    24901 gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc tctgtgccat ctctttcagc
    24961 gtcttgcagc gttcgcccag cagtttcacc agatcagcca gctgcgggcc gttacgggta
    25021 tcgatatttt cctgctcaat gtgccactgt aagtgagtag caacatactc cggcggcagc
    25081 gcgttaatgt agtgatggtt cagccacagc agcttgtcgg tgttgaacgc actggcagat
    25141 ttgctgacgg cattcaaagt gaagtatttg atcatctctt cacgagtgaa gatttcctga
    25201 tcgccgtggg accagcccag acgcaccaga tagttcagca gtgcttctgg caaataaccg
    25261 tcatcacgat actgcattac gctgactgcc ccgtgacgtt tggacagttt tttgccgtca
    25321 tcgccgttga tcatagaaac gtgcgcgtaa accggtaccg gcgctttcag ggctttaaga
    25381 atgttgatct ggcgtggcgt gttgttgata tggtcttcgc cacggataac gtgggtgatt
    25441 tccatatccc agtcatcgac aaccacacag aagttatagg ttggggaacc atcggtacgg
    25501 cggataataa gatcgtccag ttcctggttg ctgaactcga tcggaccacg gatctgatcg
    25561 tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta cgacacacgg ctcatcatca
    25621 gcgtgatgct catgactgtg gcggcagcga ccgtcataac gcggcttctc acctttcgcc
    25681 atttgctctt cgcgcagcgc ttccaggcgc tctttagagc aatagcattt ataagctgtg
    25741 ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat caaaacgttt ggtctggtag
    25801 tacggacctt catcccactc caggctcagc cagttcatgc catccataat ggcttcgata
    25861 gcttccggcg tggaacgctc aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg
    25921 tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag
    25981 cctgttgggc ttggcgcgaa gcgagttttg attttcatga aatggcctta cgtttagaaa
    26041 gatgccgaca accggcaaat cctggaaaaa ttgagtgggc gatattctat cactcccgcc
    26101 tgattcctca atgtagttcg ggttgcaacc ttacgcatta ttgttttcgt tgacaaattg
    26161 cgcaacacgg tctgttttgc gatcatcttg cttaaattta cgacgaacga ataatttctt
    26221 tagaaaatgc gttgactcat tttgaactct ccctataatg cgactccaca cagcgggggt
    26281 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    26341 catcacccac caactactat atgtagtctc cgccgtgtag caagaaattg agaagtgggt
    26401 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    26461 catcacccac cactttctca ccagctaaat ttcttgtaaa aatgtgaagt accgaagtgg
    26521 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
    26581 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
    26641 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
    26701 tttttgctat ctgcgatact caaagattcg aacctgcagc aggttcgagt tgagcgcagc
    26761 gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca
    26821 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag
    26881 attgattttt cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag
    26941 gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt
    27001 aacagggcat cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc
    27061 atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaaa
    27121 caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag
    27181 ttgcagagac agcaggcaga aaaggcgcag gaaaaagagc agcgtttgtc tctgaatgta
    27241 tcccttctaa atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga
    27301 tccccttccc gcccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
    27361 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca
    27421 accggcgtaa tgcgctttac caccagcggg ctaccttcag gcaatggtag cgccagtgcg
    27481 ggaagaatac tgatgccgat gcctgcggct accatcggga atagcgtcgc cggatgtcca
    27541 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
    27601 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
    27661 acataatctt ccgcggccaa agcgctatcc cgatggcaaa gcagaaagaa aggctcggaa
    27721 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
    27781 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac
    27841 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
    27901 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
    27961 tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc
    28021 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
    28081 ttttccagct ccttcacact gtgacttact gccgactggc tcaggccgat acgctctcct
    28141 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
    28201 taattcatct gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga
    28261 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
    28321 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
    28381 cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat
    28441 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
    28501 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
    28561 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
    28621 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
    28681 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt
    28741 tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat
    28801 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
    28861 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
    28921 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
    28981 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
    29041 ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg
    29101 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
    29161 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
    29221 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag
    29281 caccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
    29341 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt
    29401 gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttgtattt
    29461 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
    29521 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg
    29581 ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc
    29641 ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc
    29701 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
    29761 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
    29821 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
    29881 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
    29941 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
    30001 gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgattttg
    30061 gccccatacg ctccagtccg gtaagtttgc ctgcggtgag tttgaacaga tccgccggag
    30121 tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
    30181 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca
    30241 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
    30301 ccggacaatg cgtcgggaat acaacctcac gagtatcttc cgggcgttca gaaagcacga
    30361 cgttcaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
    30421 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
    30481 ctgcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
    30541 gaaactcgac gtcacgcaca aaggtcatct gttcctgcgc cgggaattta aacgctaccg
    30601 cccagcgcgg ggctcgcgcg acaaagccaa gctgctcctg ctgtgccagt gagttgacct
    30661 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
    30721 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc
    30781 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
    30841 gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
    30901 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
    30961 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
    31021 cttccagacg cgccgggata ttctctccgt gcagcttcag tggaatggcg cgaatggtac
    31081 gcacgttaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
    31141 aaacgccatt ttcatacaga atactgacag caaggccatc cagcttcagc tcacagcacc
    31201 aggtgacttt ctcgttgctt ttcagacggt cctgcactcg tttgttgaag gcaagaaagc
    31261 tttcttcatc aaaaacgtta tccagcgaca gcattggcac ttcatggcga atctggctga
    31321 aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg
    31381 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
    31441 tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt
    31501 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
    31561 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttaact
    31621 tcgcggatga tgtcctggta ctcacgcaat ttctgcggag tcatcatacg gcgctgatcg
    31681 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
    31741 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta
    31801 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctaaat
    31861 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
    31921 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
    31981 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
    32041 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
    32101 tgttgtgccg gttgcggtgc tgaatgcact ggctgcggcg ctggcgcgac ctgtggcgaa
    32161 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg cacgggttgc
    32221 tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggctgc
    32281 tggaccggtt gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac
    32341 ggacgagcag cctcatgctc ctgggcgtta gccggggcat gattcacgcg gtgaacacga
    32401 acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt
    32461 gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc
    32521 cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga
    32581 cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat
    32641 ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag
    32701 cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat
    32761 tgcgcaatta cccggtaaaa tacgcacaat tttcctggga ttggtcaaaa ggagctcatc
    32821 ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa
    32881 ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc
    32941 aatattttgt tgatgggggg cgcattctgg tggctcttta cgcaactcga tgtctggatc
    33001 ccgactctca tgagttatgt tccggactgg ttacaatggc tgagctatct gttgtggcct
    33061 ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat tgctaactgg
    33121 attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct
    33181 acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc
    33241 gaatggcaaa aatttgcctg gtatctgccg cgcgcaattg tattactaat tctttacttc
    33301 atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg
    33361 ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg
    33421 cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg
    33481 tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg tggcgcgacg
    33541 gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt
    33601 tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta ttccatcttg
    33661 catgtcatta tttcccttct gcatatagat atgctaaatc cttacttccg catattctct
    33721 gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa
    33781 gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat
    33841 cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg
    33901 ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt
    33961 tgaacttgtt gaaccaacca gcggtaatac cgggattgca ctggcctatg tagctgccgc
    34021 tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct
    34081 gctgaaagcg ttaggtgcaa acctggtgct gaccgaaggt gctaaaggca tgaaaggcgc
    34141 aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca
    34201 attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg agatctggga
    34261 agataccgac ggtcaggttg atatatttat tgctggcgtt gggactggcg gtacgctgac
    34321 tggcgtcagc cgctacatta aaggcaccaa aggtaagacc gatcttatct ctgtcgccgt
    34381 tgagccaacc gattctccag ttatcgccca ggcgctggca ggtgaagaga ttaaacctgg
    34441 cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg atctcaagct
    34501 ggtcgataaa gtcattggca tcaccaatga agaagcgatt tctaccgccc gtcgtctgat
    34561 ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg cggcgttgaa
    34621 actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac cgtcatcggg
    34681 tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag aattgcaaca
    34741 gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt ttttgtggcc
    34801 tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca
    34861 gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt
    34921 ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca
    34981 gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa
    35041 aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac
    35101 actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg
    35161 aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct
    35221 gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag
    35281 actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag
    35341 aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt
    35401 taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta
    35461 tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa
    35521 tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat
    35581 cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag
    35641 ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag
    35701 ccctgattaa agataagcac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc
    35761 aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac
    35821 gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg
    35881 ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc
    35941 tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct
    36001 ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc
    36061 aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc
    36121 caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag
    36181 cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag
    36241 tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag
    36301 gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag
    36361 aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg
    36421 ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag
    36481 agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa gagatcctgc
    36541 gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc
    36601 cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac
    36661 aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg
    36721 aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta
    36781 tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt
    36841 cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg
    36901 cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg
    36961 ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc
    37021 cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc
    37081 aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag
    37141 aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg
    37201 ggtttgttcg ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt
    37261 gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt
    37321 tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc
    37381 gcgcctgtag acggcaccat tggtaaaatc tttgaaacca accacgcatt ctctatcgaa
    37441 tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc
    37501 gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt
    37561 gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc ggttgttatc
    37621 tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa
    37681 accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc gccgattggc
    37741 gccatttttt tatgcttcca ccagcggtgg caaaatcaat tcatcgctct catgttgctg
    37801 ggtgtagcgc atcacttcca gtacacgcaa acccgcgcgg tgcaccgcgt cggttaacgc
    37861 cttccctttc agcaagccac tgatgagctg agcacaaaac aggtcgccag tccctttcag
    37921 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcagtga ccaccacaac
    37981 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttagtgt
    38041 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    38101 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    38161 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    38221 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    38281 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag ctcgaagttg
    38341 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    38401 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    38461 aaagacattc aggccgttct gtttgatagc aggcacggca atgctgttgc ctacgctgcc
    38521 gtacactacc tgcgactgca cggcgacgat atccgcctgc agtgccctgc tcttatcgtt
    38581 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    38641 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa ggataaggta
    38701 attcagtgaa gaaaatcatt tgtctggtca ttacactact aatgacactc cccgcttacg
    38761 cgaagttaac tgcccatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    38821 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttctc
    38881 atctgcgtct gaagctgggc aatacccgta accgcattga cactgccgag cagtttattg
    38941 ataaggttgc ttcgtcgtca tcgattaccg ggaagccgta tattgtgaag atccccggta
    39001 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacag
    39061 tgcctgcgca ataatcttaa atccccgccc tctgactaaa atgctcttcc ccaaacaccc
    39121 cggtggaaag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt
    39181 tagcttttgc cacccgcagt gctccggcaa ccgcgccgcc ggagctgacg ccacagaata
    39241 ttccttcccg caccgccagt tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca
    39301 gcacctcatc caccagagaa gcgttgaaaa tccccggcag atattccgta ggccagcggc
    39361 gaatgccggg aatgctgctg ccctcttccg gttgcaggcc gacaatggtc accggtttgg
    39421 attgttcgcg cataaagcgt gacacgccgg tgatagtgcc ggttgttccc atgctggaga
    39481 caaaatgggt aatgcgcccg ccagtctgct gccagatttc cggcccggta gtggtgtaat
    39541 gggcgtaagg gttatcggga ttattgaact gatcgagcag ctttccttca ccacgatccg
    39601 ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg accagaatca
    39661 gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca
    39721 gcaatttcat gcgatagcct ttaagcgcgg caatcattgc cagcgcaatg ccggtgttac
    39781 cactggtggc ttcgattaac acatcacccg gtttaatttc cccgcgcttt tccgcctcga
    39841 cgatcatcga aagcgccgca cgatctttta ccgaacctgc cgggttattg ccttccagtt
    39901 ttaaccacac ttcactgccg ttattcggcc ccattcgctg caacttcacc agaggcgtat
    39961 tgcctattgt ttgttctaat gtactcacga tctctatcca tacgtggtgt tgcctgatgc
    40021 gacgcttgcg cgtcttatca ggcctacacc attaaaaagc ccggatcgcg ggaagcgcct
    40081 ccgggcgttt aacattcact caacctatca ggcgctttgt gcgagagcaa gttcctcatc
    40141 tcgggtttcg atacgctcgt cgccgttata cagccgcgca tgttgcagac caacgaataa
    40201 acgctcgcca cgctgcgggg cattgtcgcc atgcatcacg accgtcagcg gttcgttgta
    40261 ccaccccagc ggctgcacca ctaattgggt gtagtgacct ttcgggctgg cttccagtac
    40321 ctgtaccggc agcggcgaat cgaggctggt acggcggctg atatccactt cccaagggcg
    40381 caggaagaga tccaccggcc cctgatacgc aggtgtgtag cccagcggcc agcgatgcgc
    40441 gccaacatgg aactgcccgc cgcgaatggt tccctgcagg cggttcactt cgcccataaa
    40501 ttcgagcaca aaacgggtcg ccggttcgcg ccatacctga tccggcgcgt cagcctgttc
    40561 aatattgccc tggctcatca caactacacg atcagctact tcggtcgctt cttcctgatc
    40621 gtgggtcaca aaaacgctgg tgaattttag ttcttcatgg agttgacgca gccagcgacg
    40681 cagctcttta cgcacctgcg catccagcgc gccaaacggt tcatcaagca gcagaatttg
    40741 cggttccaca gccagcgcac gcgccagcgc cacacgctgt ttctggccgc cggaaagctg
    40801 cgccggataa cgatccgcca gatgagcaag ctgcaccatc tccagcaatt ttgtcacttt
    40861 cgctttgatc gccgcggcat tcgggcgctc gcgacgcggc agcaccgtca ggccaaaagc
    40921 gatattgtcg aacaccgtca tatggcggaa cagcgcgtaa tgctggaaca cgaaaccgac
    40981 tttacgatca cgtgcgtgca ggcggctcac gtcggtgccg tggaagcgaa tatgcccgct
    41041 ggtttgatgc tccagcccag cgataatgcg cagcagcgtg gttttcccgg aaccggacgg
    41101 ccccagcaac gcgaccatct gacctgaagg aatatccagt gagatatcgt tcagcacctg
    41161 ggtgcgacca aacgacttct taatattggc aatctcaatg ctcatgattt tcctcctgct
    41221 gcgcgcgttt ttcctgattc tccaggcgcc actgcaacat actctttaaa aacagggtga
    41281 taatcgccat cagcgtcaac agcgcggcgg cggtaaagga gccgactgtg ttgtagtcct
    41341 gctccagcaa ttcaatctgt aacggcagcg aaagtgtttc gccacgaatc gagccggaaa
    41401 ccaccgacac cgcgccaaac tcgccaatcg cgcgggcgtt ggtcagcact acgccataaa
    41461 gcagcgccca gcggatgttc ggcaatgtga cacggcggaa catctgccag ccggatgcgc
    41521 caagcaaaat cgccgcttcg tcttcctgac tgccctggct taacatcacc ggcaccagtt
    41581 cgcgcaccac aaacggacac gtcacgaaga tagtgaccag caccattccg ggccaggaga
    41641 acataatttg cagattatgt tcatccaacc agccgccgag cgggccgttg gagccgtaga
    41701 acagcagata caccagaccg gcaaccaccg gtgacacggc aaaaggaatg tccagtagcg
    41761 tcagcagtaa ctggcgtccg gggaagttaa agcgcgtcac cagccaggcc agcagaatgc
    41821 cgaacaccag atttaccggc acggcaatca acgcaatcat caccgtcagc cagatggcgt
    41881 gcagcatgtc cggatcggcc agattctgta aaaccggcat cagccccttg ctgaatgcct
    41941 gcacgaagat gtaaatcatc ggcaccagca agatgaacgc cgaaaccagc atcccgatgc
    42001 caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc aactgtgtca
    42061 cttccgccat taatgaccta ccacacgccg accaaagcga ctttgcagag tgttaattga
    42121 gaacagcagc agcagggatg ccgcgaggat caccgaagca atcgcgctcg ccgccgggta
    42181 atcaaactcc tgcaagcgca caaagatcat cagcgacgtc acttctgtct tccaggcgat
    42241 attcccggcg ataaaaatca ccgcgccaaa ctcaccgagg ctgcgggtaa acgacagcgc
    42301 cacacccgcc accagcgccg gagaaagctc cggtaacacc actttgcaga aactctgcca
    42361 gcgcgtggca ccgagcgttt ccgccgcttc ttcatattcc gggcctaact cttccagcac
    42421 cggctgcacg gtacgcacca caaacggaat gctggtaaag gccatcgcca ccgcaatccc
    42481 aagccaggtg taggtgactt tgatatcaaa cttcgccagc cattcaccgt aaaaaccgtt
    42541 tacggaaaag agcgaggcca gcgttaaacc agcgactgcc gttggcagcg caaagggtaa
    42601 atccatcagc gcatcaagca gcgtgcggcc agggaagcga taacgggtta aaatccacgc
    42661 catcagcagg ccaaaaacgc cgttaaaaat cgatgccaca aacgctgaca gcagcgtcac
    42721 tttgtaggcc gcgaccacct gcgggttggt gatcacctcc cagtactgcg cccagctcat
    42781 ctgagccagt tgcatcacca gcgcggagag cggcagcagc aaaatcaggc acacaaacag
    42841 cagactggtg ccgaggctta aggtaaagcc cggcagcaca cgtctggaag agacagcaaa
    42901 catcagttac gccccgccgc taacagcttg tctaactcgc cgccgctggt gaagtgggtt
    42961 ttcatcactt ccggccagga gccaaacttg tcttccacgc ggaacaactc ggtctgcggg
    43021 aatttgtctt tcagtttgtc catgacttcc gggttattca cgcggtagta atagtcggtg
    43081 atgatggttt gcgcctgcgg gctgtagagc cagttcaggt aggcttttgc ggctttttcc
    43141 gtaccgttgg cctgcacgtt tttatcaacc catgccaccg ggaattccgc cagaatgttg
    43201 gttttcggaa tcaccacttc aaaaccctgc gcttcatact gtttacggat gttgttcact
    43261 tccgactcga agctgatcag cacatcaccc aggccgcgct cggcaaaagt ggtggtcgcg
    43321 ccacgaccgc cggtatcgaa cacttcaacg tttttcagga actgagtcat aaactgttcg
    43381 gttttggctt tatcgccacc gtcggctttg tccgctgcgc cccacgcggc taaataggtg
    43441 taacgcgcgt tacccgacgt tttcgggttc gggaaaatca gcttcacatc ggagcgcacc
    43501 aggtcgttcc agtcgtggat attcttcggg ttgcccttac gcaccaggaa gcccattgtg
    43561 gagtagaacg gcgagctatt attcggcagg cgcgtttgcc agtccgccgg gatcagcttg
    43621 cctttgtcat gcaggatttg tacgtcggtc acctggttat aagtgacaac gtcggctttt
    43681 aagccctgca aaatcgccag cgcctgtttt gatgacccgg catgagattg ttttatcgtt
    43741 agtttatcgc cgccgttatc tttggcccat tgttgctcaa acggcgggtt cagggcggca
    43801 aacagctcgc gggagacgtc ataagaactg ttcagcagtt ccgttgcctg tacatggccc
    43861 gccagcagca gagaagcgac cagcgcgagt gagttctttt tcagtaagtt aacggccatt
    43921 gcgcaccctt atatatttaa tgactttcta atagccatca tatttataac ggacgtgaaa
    43981 ggagtaacgg ttttatatac cgtttggtga tttggaagtt gaaaagggaa taagaacttg
    44041 gttcaacagg cgtaattgtt gcagtcagtt tggacacgaa cagcgcggag aaaccggagc
    44101 gtacacatag tacgtgagga tttcgagcac tgcccggggc caaaatgaca aataaaatag
    44161 cctggtgaac gtagttcaag acgaaatcct ccccacaaat gcagggagga aacagaggtg
    44221 aatcagatac cgacgctaac cgtctccggc agtgtgctgc cgccatcaat cacattctgt
    44281 gtaccggtta aatagctgga ttcatccgat gcgaggaagg ccgccagttc gccaacttcc
    44341 aacggatcgg cgaggcgacg catcgggatt gctttcgcca tttcagtcag caccgattct
    44401 ggatcttccg ggttcgactg gcgggcaatg ctttccgcca ttggtgtgcg aacgtatcca
    44461 gggcatatgg cgttaacgcg aataccagac tgcgcatact ccaccgccag cgatttagtc
    44521 aggccaacaa tcgccgcttt cgttaaggca tacgccgttt cacccggatc ggccaccata
    44581 tcgccagtga ccgaagacat catcacaatg cgaccatctt tgcgggcaat catctccggc
    44641 agcaccgcct tcgtgacgtt ccatacgcct ttaatattga tatcaatatg gaaatcgcga
    44701 tcttcatcgc tcatatcgag gaaactgccc agacgacaaa cgcctgcgtt attcaccagg
    44761 atatcaatgc gcccttcttt ttccttcgcg cgtttgatag ctgcggctac cgacaccggg
    44821 tcacgcacat cggcgacaac cgccgtacag cgatgaccac gaccacacag ttcgtccgcc
    44881 agcttttcga tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa
    44941 gttctggcaa ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc
    45001 gtgagtttac ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc
    45061 tgaaaataca ggataaacaa aatattgctg cggttcagct cactaattca ggaaaatctc
    45121 aatccagtcg ctgctttatg catcatttca ggcgttgtgt tagctcactg ctgcgctgaa
    45181 tcattttcaa tgactcgaca tcattgagtt gcacaaggcc aacaaacagt aaatcggtaa
    45241 cagaattttg cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcgccagaaa
    45301 cggtatcgag ggtgtaatgc gccagccgcc gcagcgggga atcggttaac gaggtaatgg
    45361 caatcaccgt tgccccctgc tttcgtgctg cctcggcgca caagacaatc tcttttttac
    45421 tgccgctgta agagatagcg atttgtacat cgcctttttt cagcgcctgg gaaacggtgg
    45481 cttgtacatg tgtgtcggct tcgcaggcca cgcgatagcc aattttcatc agtttgaacg
    45541 acagatcgcg ccccaccagc gccgacccgc ccaggcccgt tatctggata aacggtgctt
    45601 tactgatgac ctcaatgatt ttttgcagcc gcgcgaagtc aaacaacgcg caggtttgct
    45661 ccagcgccag ctgcttttcg cgattcagtt tacgggcaat cacttccagc gaatcgtcac
    45721 tggtaatcga actgtgcagg tgcagcgccg tagcgttcgt tttctcccgg ctggcgctgt
    45781 attcgccaat taacgccata cgcaattcag taaacccttg cgcgccgagc ttctgggcaa
    45841 acttcacaat actcgactgg ctaatgccaa gctgtttcgc catcttgcgc gaagagacgg
    45901 atttcagctc actcacgttg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa
    45961 attcactttc cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg
    46021 cagcaaaaat tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa
    46081 tgattccttt aagtcggtca atacgggata tatgattcca tacatatgca attatttgat
    46141 cgtaaatagt aaggtcacca ccgatgcaac ttgaaaagat gattaccgaa ggctcgaacg
    46201 ccgcttcggc tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg
    46261 aagataaaac cgtaccactc gccgttgagc gcgtactgcc ggatatcgcc gcggcgatcg
    46321 atgttattca cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat
    46381 ccggtcgtct ggggattctg gatgccagcg aatgtccgcc gacctacggc gtgaaaccgg
    46441 gtctggtcgt gggtttgatt gctggcggcg aatatgccat tcagcacgcg gtggaaggcg
    46501 cggaagatag ccgggaaggc ggcattaacg atctgaaaaa tattaattta acggcgcagg
    46561 atgtggtggt tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat
    46621 atgcacgcca gctcggctgc cgtacagtgg gaatttcctg taatccgggg agtgccgttt
    46681 caaccaccgc cgagtttgcc attacaccga ttgtaggtgc cgaagtggtc actggttctt
    46741 cgcgaatgaa agcaggcaca gcgcagaaac tggtgctcaa tatgctctcc accgggctga
    46801 tgattaaatc cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa
    46861 aactgcacgt gcgccaggtc aatatcgtca aaaacgctac cggatgtagc gcagagcaag
    46921 cggaagcggc gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga
    46981 aaaatctcga tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgca
    47041 aggttttaga caaggaataa ctcatggcca aagagatcag cagtgaactt ctgaacacca
    47101 ttcttacccg tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc
    47161 tgcgtctggg tgtacatgac agttcactgg ttgatcccga tatcaaaacg ctggaaggcg
    47221 tgaagggcgt cattttgacc agcgatcagg tgcaggtggt ttttgggcct ggtaaagcgc
    47281 atcgtgccgc aaaagcgatg agcgagctgc tgggagaagc accggtacag gatgccgcag
    47341 aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat
    47401 ttctcgccaa attcgccacc atcttcacgc cgcttatccc cggttttatt gccgccggtc
    47461 tgctgctggg gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg
    47521 gcacgctacc cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc
    47581 tggtgattct ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg
    47641 caattattgc cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg
    47701 ccggttttca cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgt
    47761 tgattgccgc gtgggcctgc gctcgcattg aaggcatggt acgccgcttt atgccggacg
    47821 atctcgacat gctgctgacc tcgttaatca ccctactgat caccgctacg ctggcctacc
    47881 tgatcattat gccgctgggc ggctggttat tcgaaggtat gtcgtggctg tttatgcacc
    47941 tgaacagtaa tccgctcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat
    48001 ttggcgtgca tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca
    48061 acagcttatt tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac
    48121 tctactggcg ggcgcaaccg cacagtgcat tacgcagtca ggtacgcggg gcgattattc
    48181 ccggtctgct tggcgttggc gaaccgctga tttatggcgt caccctgccc cgcatgaaac
    48241 cgtttattac cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct
    48301 ggtggggtct gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc
    48361 cgctgatgac ttcctcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc
    48421 tggtggcatg ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc
    48481 tggactgata tcatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc
    48541 gtcagcgccg caaaataccc tgttctgaca gaaagttcgc cagagaaagc agggttcaac
    48601 gtcgaacggc ttaaccagat ggatcgctgg attagccagc aagtggacgc cggttatccc
    48661 ggcgtaaacc tgctgattat aaaagataat cagattgtgt atcgcaaagc ctggggagcg
    48721 gcgaaaaagt acgatggcag cgtattgatg gaacagcctg tcaaagccac caccgggacg
    48781 ctgtatgacc tggcttccaa taccaaaatg tacgccacta actttgctct gcaaaagcta
    48841 atgagcgaag gcaagctgca tcctgacgat cggattgcga aatatattcc gggatttgcc
    48901 gatagtccaa atgacaccat caaaggcaaa aacacgctgc ggatttctga cctgctgcat
    48961 cacagcggcg gtttcccggc ggacccacaa tatccgaata aagcggtcgc gggcgcgtta
    49021 tattcccagg ataaaggcca gacgctggag atgatcaagc gcacgccgct agagtatcag
    49081 cccggcagca agcatattta cagtgatgtc gattatatgc tgcttggatt tatcgtcgag
    49141 tcggtcaccg gtcagccgct tgaccgctat gttgaagagt cgatttatcg cccgctcggc
    49201 ctgacacata cggtatttaa cccgctgctg aaaggcttta aaccgcagca aattgccgcc
    49261 acggaattga acggcaatac ccgcgatgga gtgatccact tcccgaatat ccgcacctcc
    49321 actctctggg gtcaggtgca cgatgaaaaa gccttttatt cgatgggcgg cgtttccggg
    49381 cacgcaggtt tgttttccaa taccggcgat attgcggtgt taatgcaaac gatgctgaac
    49441 ggcgggggct atggcgatgt gcagctgttc agtgcggaaa cggtgaagat gttcaccacc
    49501 agctctaagg aagatgccac ttttggcctc ggctggcgcg tgaatggtaa tgccaccatg
    49561 acgccgacgt ttggcacgct ggcaagcccg caaacctacg gtcacactgg ctggaccgga
    49621 acggtgaccg ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca
    49681 cattcgccgg ttgccgatcc gcaaaagaat cccaatatgt tcgaaagcgg tcagctgccg
    49741 attgcaacgt atggttgggt agttgatcag gtgtatgcgg cgttaaagca aaagtaataa
    49801 aaaggcctgt cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag
    49861 cgccatcaac ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa
    49921 acgcaacatc gcatcacgct taccatcggt atcgccaaac atgctcagca gttgctgctc
    49981 aatgttatgc agacgcgcgc agtaggcgca gaagtacaga ccgtgcgtac cactggcggt
    50041 gccgtacggc aggctctgac gaacaatttt cagccctttg ccatcttctt tcagatcaac
    50101 gcgggtcagg tgagaagttt ccgggcgttc gtcgccgtcg atctcttcgt tggcctcttt
    50161 ggtgcgcccg atcatcatct cctgatcgtg aacgctcata cggttgagct gcttcaggtt
    50221 gtgctcccaa cgctggacaa acacatagct gccgcccgca tccacgccgt ctttgataac
    50281 cgccacttcg cgacgcgtct cttcacccgc cgggttttcc gtaccgtcaa caaagccgct
    50341 caggtcacgc tcttcaaccc aacggaaacc gtggatctct tctttcactt caatgcagtc
    50401 gccgaaagct tccatcgccg cctgagcgac agagaagttt acgtcgtgac gcaaagaaag
    50461 aatgtggatc aacacatcga actgggtcgt tggcgcaagg cctttaccgt agcccggaaa
    50521 atctttcagc tcttcggccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa
    50581 ggcaaccacc gcaccaagat gcgcgtccgg gaatttcgct tcaaaagtcg ccagtttgtc
    50641 ggcaaatgtt ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat
    50701 ccaaatcgcc gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc
    50761 tcctgaaaat aataatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt
    50821 ttcgcggcgg cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc
    50881 cagtttttca gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg
    50941 aactggtagc taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca
    51001 ccatcagctt tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg
    51061 attttaacac cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg
    51121 ccttgatcgc cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc
    51181 accacgttgc cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca
    51241 tcggcaatgg cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg
    51301 ctcactttgg tggacgaccc aaaccagttc cacgggttag cggcagacca gttaactgac
    51361 gacatcgtgg aacagccggt cagcatcaat ggcatagcgc ataacattaa acgcagcgat
    51421 ttcatgtcac ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag
    51481 tgccgttatt cttttacttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag
    51541 attaacgcca caatatccgc cactaccagc gccagaccga aaccgctaac ggattcaccg
    51601 ttcagccaca gccacggttg ccagcaaagt aaaaccacct gcgccaacag caacagaaaa
    51661 tagagtacac gccaggtgcg aggaaacgta gcccgccgac cgctcaacaa aaacgccagc
    51721 accgccggaa tgccaggaat cagccccagc cagaaattat cgtgatcggg ataaaacaga
    51781 tttagcagcg cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt
    51841 cgcgcctgaa gcaatagcac aagccagaag agcaagggta aacgcaggcg accgtgcgca
    51901 tcataatgga caggatgaaa ctcagtactc ttcatcttca atcaaacgct tacccagact
    51961 cagcacgtcg gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc
    52021 ttccggcacg ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccagccg
    52081 gttaagcaac gcattggcaa tcccacgccc acgaaactct ggatgcacgc caagataata
    52141 agcagacccg cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgttcac
    52201 ctcagcgacc agaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg
    52261 atcgttccac ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc
    52321 ttcctggcga aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct
    52381 gattatggcg tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc
    52441 ataatgggga gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaacggt
    52501 cgtaacggat tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt
    52561 atgagcactt ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt
    52621 ttggctgccc tgacgttgtc aggaatgtcg caagccatcg ccaaagacga acctttaaaa
    52681 accagcaacg gacacagcaa gccgaaagct aaaaaatctg gcggcaaacg tgtcgttgtt
    52741 ctcgatccag gccacggcgg gattgatacc ggagcgatcg ggcgcaacgg ttcgaaagaa
    52801 aaacatgtgg tgctggcgat tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt
    52861 gatgcgcgtt taacgcgttc tggcgatacg tttatcccac tttacgatcg cgttgaaatc
    52921 gcccataaac atggcgcaga tctgtttatg tcaattcatg ccgatggctt taccaacccg
    52981 aaagctgccg gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg
    53041 aaatacctgt ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac
    53101 aaggatcacc tattgcagca agtgctgttt gatctggtgc aaaccgatac tattaaaaac
    53161 agcctgacgc ttggctcgca tattctgaag aagattaagc cggtgcataa actgcacagc
    53221 cgcaacaccg aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg
    53281 gtggaaacct cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcgttt
    53341 cgtcagaaaa tcgccacagc gattgctgaa ggcgtgatca gttatttcca ctggttcgac
    53401 aaccagaaag cacattcgaa aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa
    53461 cagtttctgc tcaaccttca ggatacgatt tgtcagcagt tgaccgccgt cgatggcaca
    53521 gaatttgtcg aagatagttg gcagcgcgaa ggtggcggcg gcgggcgtag tcgggtgttg
    53581 cgtaatggtg gtgttttcga acaggcgggc gtcaactttt ctcacgtcca cggtgaggcg
    53641 atgcctgctt ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg
    53701 ggcgtttcac tggtagtgca tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg
    53761 cgctttttta ttgccgaaaa accgggtgca gagccagtct ggtggtttgg cggcggcttc
    53821 gatttaaccc ctttctatgg ttttgaagaa gatgccattc actggcaccg caccgcccgc
    53881 gacctgtgcc tgccgtttgg cgaagacgtt tatccccgtt acaaaaagtg gtgcgacgaa
    53941 tacttctacc ttaaacatcg caaagaacag cgcggcattg gcgggctgtt ctttgatgat
    54001 ctgaacacgc cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac
    54061 accgacgctt atttaccaat tgtagagcga cggaaagcga tggcctacgg cgagcgcgag
    54121 cgcaattttc agctctaccg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc
    54181 ggcactctgt ttggcctgca aactggcggg cgcaccgagt cgatcctgat gtcaatgccg
    54241 ccactggtac gctgggagta tgattatcag ccaaaagatg gcagcccaga agcggcgtta
    54301 agtgagttta ttaaggtcag ggattgggtg taactccctc acccccattc ccgcatccgc
    54361 tgatgcaacg tcaacgacgg cttctcggca aacagctgct ggtaatccgt ggcaaattgc
    54421 cccagatgcc agaatcccca ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac
    54481 gggcttatca gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca cgcgttcggg
    54541 ccaatgccta aaatagcgtg aaacgcgttt tgtagcgtgc ggcggctgac atgcagttga
    54601 ttacacaagt ccagcaccgt caccggttcg gacatgtttt ccagcacata ttcacgggcg
    54661 cgggaaagta atctacggta actctgatga ctgatgcttt ccgccgtcac cattggttgc
    54721 gcttcttcca gcatcgcccc catcgccatt agcaaattat cccccagcac ttttcgcact
    54781 gcaggctgat ggagattttc cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag
    54841 ccccacagcg cggctttatg ctgctctttc acttccagcg ccgattggct acgcagcata
    54901 tgtaataccc gatccggatt gtgcaaaaag ttagcctgac gggtgatgac atcttcagac
    54961 agcactacgc ccaggatcgt gtaatcgtcc ggcgtactta attcaaactc ggttccaccg
    55021 gggcgcgtcg ctatttctgc gcttcccaga cattgcgaac cgataaatcc ctgctcaccg
    55081 cgcgtcgccg gaatgccaaa ccagaacgag ttaggccaga ccaggcacga ctgacgcagc
    55141 gccagaccgg tgtattcacg aaaaacctga atatcatcga gtagaatttc cgtaaattca
    55201 ccatgaaact tgcccggatg cagctgatcg taaatctgct gccaggcggt aatcgttaaa
    55261 gcatgttcat agacatccgt tgtctgtcgt tgatgaacat tatccacttc gaccttcggc
    55321 gtgagcttca ggttttcggg taagggttca tgataaagat ggtgcaaatt ggctgtacgg
    55381 gtctttttca tgatgttaat gccgggtgtt gtaggacacc cggcacctcc gacaggttaa
    55441 tggggcttga gacgataacg actactgcgt ttacgtaacg tcccggcaga aaagagctgt
    55501 tcgagcgcat ttctggcttt ttccagcggc cagccaaagt gggcagccac ttctcctgcc
    55561 gtcattccct gacgtactga tgttaacagc gccaacaact cgtcagcaga ttccgcgacg
    55621 ataactggcg cgtcgggttc ctttacgggt tgcttcggct ggcggttaaa gccagtaacc
    55681 agccactggg tgtcatcgtc tggccgaccg atctccttgc ggctgatgac acgcccggta
    55741 cgcatcgcgg cagcacaacc tgcgtccagc gcagcacgac acgccgccag atcgccttcc
    55801 accaccagcg ttaagcgacc agggtcaagc acttcgtgac tgagcagacg aacgttagct
    55861 gctttgagca tggcatccgc tgcatctatc gcagcgacca tgccgtccac ttccagcaat
    55921 cccagtgcat tgatcatcgg caacctccat tacgcacgct ggattggatt acgcgcgatt
    55981 tccagcactg catcggtaaa ggcgttacag gccgctttac acgctgcctg gctaccggtt
    56041 aaaaatgctg ccgagtagtt agtttcagac ggtggcggga cataggtcac cagctgtacg
    56101 tcggcagatt tcaacgctgc atcaatgccg taggtcgctt ccagcggcgg tgccaccaga
    56161 tacgccatcg gatcgccaag cgtgatcccg gcggttgatg agagataaga accggtacgc
    56221 gaaactacat gtgccaggaa tgccgtatct tgcgcatcat tagcccactg gaaagcagcg
    56281 ccattttcaa tatgcgcaac catcgcatcc agaccggcac gcacttccgc cgggtttggc
    56341 ccaccaagca taatcagcac ttcaccggca gtcggtgacg ggccgtgtgc agcgcccgca
    56401 tacagcgagc ggccatacac cacttccacc atcgcctgct tggtcgcttc atcagcggca
    56461 atgtaggtga cgtcatcgga gtcggcagaa atcagaccga ggctacgaat atgcggcggc
    56521 aatttcagtt cacgcgcaaa atcggcgtta acggaggcaa tcacccgcat ggcggttacc
    56581 gacggtcgaa tcaaatctaa agctggcatg atgcctcctt atcgggtcat gttgatgccg
    56641 gatgctttct gctccagcat acgtttcgcc aaatccacaa tgacggctgc ggcttcaacc
    56701 ggcggcgtgc cgccctggtg aatgttagag atgcaggtgc gatcggcctc aacggtggtc
    56761 gccatacgcg gcgagtaaac ggcgtagcag gagaggcttt ctgactgacc caaccccgga
    56821 cgttcgccca ccagcaggat cactactttc gcgccgagga tctcaccgat ctgatcttca
    56881 atcttcacgc gaccataacg aacaaagaat ggtgtaccga ctttcagacc ggcctgtttc
    56941 aggcccgcca tcagcggtgg caggatctct tcgtagttca cggtgatcgc atcagttgac
    57001 aggccatcag aaataacgac ctgcacgtcc ggattggcaa cacactgcgc tttcagcgct
    57061 tcaacagctt ctgcacacag gcggcggccc atatccgggc gagtcaggta gagatttttg
    57121 tcgctaattt cggagcggac ttccagcaag ccttgtgctt tcacccactc ttccggcact
    57181 tctttcagta cggtatcttt cgaacgagag tgatcggcca ggaaacgcag cagcgcctgc
    57241 gtacgtggac gcggaccggc acgaccggta caaacgcggg ccacggtgct gcggcgcagt
    57301 tctgttaata cgtctgcgcg atgcgggttt tcaacaccaa tccacgcttt tgcttccgca
    57361 gaacctaaat ccagcgcgca gctttccgag gtcaccggtg ccgcacaggt ggtggtggtg
    57421 cactttgctt ctgacggcgc gggggccgtt tgtcccattg acgccatcac gctgcgtaca
    57481 atttcttcaa tctgtttttg atccatgatg tgttatctcc gcgtcatcag aagaacagtg
    57541 acggatcgcc cgcccgtttg gtcaggcgac cgtttgccat aatgcccatg ctttccagcc
    57601 agcgttcaaa ctccggtgac ggacgcaggt tgagtaactg acgcacagtg gcggtatcgt
    57661 ggaatgcggt ggtctggtag ttgagcatga tgtcatcgcc caacggcatc cccatgatgt
    57721 agttgcagcc tgcggtggcg agcaggatca tcaggttttc gttgaggttc tggtcagcgt
    57781 cagcgtggtt ggtgtaacag cagtcacagc ccatagagat gccgctcagc ttgcccataa
    57841 agtgatcttc taagcccgca cggataatct ggcggtcgtt gtagagatac tccggcccaa
    57901 taaaaccgac cacggtgttg acgataaacg gatcgtaatg acgcgccagc ccatagttac
    57961 gtgcttccat cgtcacctgg tcagcgccga agttagcgcc agcggatagc gcagagcctt
    58021 gtccggtttc gaagtagagg cagttttccc cggcgatacg gttgaactct gcgcccactg
    58081 cgcgcgcttc gtcgagcatc gccagttcca cgccaaactc tttcagccct ttttcgctgc
    58141 cacagatact ctggaaaatc agtccgccag gcgcgccgcg acgaatcgct tcgatctggg
    58201 tggtgacgtg cgccagtacg cagccctgag ttgggatgtt gaatttgtcg atcacgccat
    58261 aaatggtatc cagcacgcgg cttaagtttt ccacatcgtc agtcaccggg ttaacgccga
    58321 tcaccgcatc gcccaccccg aaggaaagcc cttcgtagat ttgcgcggcg atactttgca
    58381 cgtcgtcacg ggtgtcattt ggctgcaaac gggcgctaaa ggtgcccgga ataccgatgg
    58441 tggtattggc ctttttgatt accggcattt tcttcgcgcc gtagatcagg tccgcgttgg
    58501 agcaaatctt cgctaccgcc gcgaccactt ccgaggtcag ccctttgcgg gtaaaggcaa
    58561 tgtcgtccac gctggtttca tcgctcagca catactcacg cagttcgctg atgctccagt
    58621 ttttaatctg gttgtaggcc gtttcgttaa catcgtcctg aatcagccgc gtcacgcagt
    58681 catcttcata ggcaatcacc ggattattgc ggatgtccgc tacggtcatt tccgacaaca
    58741 cctgctttgc cgccacgcgc tcctgtgagc ttgccgctgc gacgcccgcc agcacatccc
    58801 ccgaacgcag ttcgttggct ttagccagca cctcttttac atccttaaac tgatatacat
    58861 tgccgaacaa tgtggtcttt agtttcataa gtcgttccct caggaaggaa atgcgagtga
    58921 tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt cgatatagtc
    58981 ccccgcgcag acaatcactt cgtcaatgac tgccaacggg agttgttgta gctgtgggcg
    59041 caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca ggggatgcgg
    59101 attcggaaaa cgcgcgacga aatcgaccaa cgcgttgatg accgtcagta ccgcggcgta
    59161 acgcacaggc agcgaggcgg gaagcgccag cacgtacgcg tcagttttgg gatcaagatc
    59221 cagctgaagc agcgcctgtt gccaggcact caccagatcc gtttcatcaa ttgggatcgc
    59281 caccggcaaa ttgcgcagcg gcagttgtac gccctcaagc cagattgtgc tgccagagag
    59341 cgaaagggta tgtgcacccg caccaatcac cgtggcgcgc acggtttgcg ccggaaactg
    59401 tacgttcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagca gcgggccaat
    59461 atcggcaaaa cagaacgggt cggcgggctg gtggcgataa cattcgccca cgccgccaga
    59521 aagcgtaatg atttcgggcg taacacctgc gggcagcaaa ccggtttgca ttaatgcctg
    59581 cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agctctgcca tccgccgggt
    59641 aacctgcacc agctgagcgc cggtcagcga acgggcgtcg gtgcctgcgc cgaagcactc
    59701 atccacaatc atctgccccg gtttatgagc gtaaaccacg cgcccctggc tgtcggtttc
    59761 cagcaggcga ccaccgacgt tgaggcaggc agtgccgctg atttttccgg catcgaacag
    59821 ggcgtagttc gcggtgccac cgccgatgtc gatattcagt acccgacaca gccgttgttc
    59881 agaaagggtt tgtgccccgg ctccgtgacc ggcgatcacg gattcgaggt gcggcccggc
    59941 gctggcaacg acaaaatcgc ccagcgactg agagagcgcc atcaccgccg ggcgagcatt
    60001 gcgggttttc gcgctttcac cggtgatgat gatggcacca gaatcaacgc tttccggcgc
    60061 aatgcccgca gcctgatatt gctctaaaat cagcgatttc agttccgctt cttttaaacc
    60121 accctgttta tcgacagggg taaagaacac cgggctttgc cagctaattt cgcgtttgat
    60181 gaattcgtag cgcggcacct gcgacaccgc cgcacggtta accagttcca ggtgggagaa
    60241 aatcacctgg gtggtggtgg tgccgatatc gataccgacg ctcagtagct ggcgagtgtt
    60301 cacgattgtg cctccgcttc ggttttagcc gcggtcgcgt cgtcttttgg caccagcatc
    60361 atcgccacgc caatcgccgt tacgccgccg atcaacttgc cgacaatcat cgggaagatc
    60421 attgcattca tgttggcagc ggcgaagcct aagtggtcgc ccagggcgaa agcagcggaa
    60481 acggcgaagg cgcagttgat gactttgccg cgggtatcca tctgcttcat catgccgaac
    60541 atcgggatgt tgttggcaag cgttgccacc atgccggctg ccgcgatgtt gttcatattc
    60601 agcactttac cgacgctcat cagcggtttt tcaaaccagc gggtcagcag cagcaccatc
    60661 ggatacgccc ctaacagaac gcaggagata gaaccgataa cttcaatggc gcgcatcacc
    60721 tcaccgggtt tatcgccagg ggccataaag ataggatcca gaccggggat cagttcccag
    60781 ccaagcagga atttcactac cgcagcggca agaccgaggg tgatcaatgc aacgaggaat
    60841 ttggcgaaga tctggaagcc gttgatcatt ttttccggga tgaatttcag ccccagcgcc
    60901 accagaatcg caacaatgat caccgggatc atgttcatca ggatcagggc gaaagtgaat
    60961 tccaccggct ggccgttgat ctgcacaccg gagtacatag ctaccagacc accagcaata
    61021 cagccaatcg gaatggtcac aatgcccgcc agcacgccga gcgccagata acgacggtca
    61081 gaaggttcga taatgccgag cgccaccgga atggaaaaca caatcgttgg ccccatcatc
    61141 gacccgagaa ttaaccctga gtatagccac gctgctacgt cgccgcccgc cagctctttg
    61201 gcgaggaaga agccgcccat atcgcacgcc agcagcgttc cggcgaacat tgacgggttt
    61261 gcgccgagca tttcgtaaac cgggataatt accggcccga gtatgtgagc cagtaccggt
    61321 gccagcgcgg tcataccgac catcgccagg cccagtgcgc ccattgccat aaagccttct
    61381 tcgaactgac cgcctgatcc ttcgatactt ttaccgaact taccgaggaa acgagcagaa
    61441 ccgccgaact gtgacaggat cctgtctacg gcagctatca gcataaagaa catcatgatg
    61501 tacatgatga tttcgttaat tcccatcgcc tttactccct gttagttgtt atttattagc
    61561 ggatgctgcg tgaacgcctt atccgcccta catgtgcaat cccgtaggcc ggataagacg
    61621 cggcaagcgt cgcatccggc aattgcaccg cgccactggc ggatgcggcg tgaacgcttt
    61681 atccgctcta catgtgcaat cccgtaggcc ggataagacg cggcaagcgt cgcatccggc
    61741 atttattgcg ccgctgcgta caggcagaca atctgctcca ggctggcggt acgcgggttg
    61801 ctgcgcagac aaatatcttc ctgcgcggcc tgcgcccatg caccgtaatg cgcagatgtc
    61861 gcgccaacat cgcccagtcg tttaccaatc ccaacttccg caatcagctc acttaccgcg
    61921 ttaatagcgt cacgatcgtc ggatttttta gttcgcagtg cccgaccaat ctgactaaag
    61981 cgttcgcgac aaaccatccg gttaaactcc atcaccgttg gcagcaacat agcgttcgcc
    62041 agaccgtgcg gaatatgcag cgccgcgccc ggctgatgcg ccatcgcgtg gcacaaccca
    62101 agacccgcac tggaaaacgc cattcccgcc atacatgaag ccagcaacat gctctcgcgc
    62161 gcggcaaggt cgtgaccgta gcccaccgct ttcggcagcg atttgccaat catcgcaatg
    62221 gcaccaatcg ccagactgtc ggtaaacggt gtagcgttca gggcgctgta tgcttcaatg
    62281 gcatgggtta acgcatcaat gccggtcatc gccgtgacat gcgacggcac accttcggtc
    62341 aatgcggcgt cgaggatcgc cacatccggc atcagcgagg catgggctaa cacctgcttg
    62401 cgcccgctca ccgcgtcgat aatcaccgtt acatttgtgg tttcagagcc ggttccggcg
    62461 gtagttggaa tggcaatcag cggcaagcgc ggttgcagaa cgctggtttc tgacatctct
    62521 gccagcgtgc tatccgggtt cgtcaccagc aacgccacgg ctttcgccgc atccagcacc
    62581 gagccgccgc caaatgcgat caccccgtca caacctgatt cacgcaactg cgccacggct
    62641 gcacacacgt cggtaatgca cggttcgccc accggacatg gccagagcgt catggcgata
    62701 cctttaacgg ccaggctacg cgtcagcccg gcggtcatcc ctgcctgatg caaaaagctg
    62761 tctgccatca cgaacagatg tttcagccca cgcgtttgcg cttgctgtcc gcaactgctc
    62821 accgagcccg gaccacaaag cgtcaccggt ggaacactaa atgtttttac ccgttgcaga
    62881 ttcagggtat cgaacgcctg aaagagcgcg gtctgcaatt cattttgcat atagcccctc
    62941 cgctttctct cgcccactac tggcgatcgc cagcggggtc ataaacaaac tgtgctgcgg
    63001 taaatgcacc tgtaacgccg ggaattgttt gcgaaacagt tctgccacgc ccggttgcat
    63061 acaagagccg cccgccagcc ataaatcagt aatgccctgc ccttcaatat gccgggcgac
    63121 aatgtccgcc attttttcgt acaccggttt caccgcgggc caaatctcgt caccgtgacc
    63181 gcgcttgtac tgctctgcct cttccagcgg aatacgacga tttccggcga gagtcagaga
    63241 gatgtgatgt ccgccagtcg cttcatccgc cgagtacgtc accttgccct ttttcacgat
    63301 ggcaatgccg gtagtgccgc cgccgatatc cactacaccg gcgttgtcca gttgcagcag
    63361 atccgcgact gcggttggct catccagcac atggctcact tccagtccgg cagattccag
    63421 cacgttgatg gaaatacgcg ggtccgtacc tggaggaaat gaagtcgccg catggctaaa
    63481 acgacgaccg aattgctgct cgagcgtgtc gagatggcga cgaacaatgg tgaccgcgcc
    63541 gaagaaatcc cagacgatgc cgtcgcgaac cacatcggcc cagtcgaggc ataccgccac
    63601 tggctgaccg tcgcggtcga caaccatcga caccacatcg caggtgccca gatccacccc
    63661 cagccacagc ggagattccg tcgcggcggg cgtctggtta cacagcgttg ccgccgtttg
    63721 cagacgtggg gtgagccatt gttcgtcgtg cgccatctgt tactccttaa acaatgcgaa
    63781 acgcatcgac taatacacag cgacgcagac ggacaaacgt ccgcgcgctg gttacccctt
    63841 caccggttgg cgtggtgatg gtcatggtag tccagccttc cccgcccaga cccagcccgg
    63901 caatgcacgg tccgttctta acgaaaatgc tggtatcaat ggcattcgcc atctggttca
    63961 tgttctcgat attgcgcgag tgcattgccg ccgtgtggtg gcaaccgccc tccagtttca
    64021 ccgctagcgc aatggcatcc gccacgttgg cgacgcgcac gacgggcaac accggcatca
    64081 tcagttcagt cacggcaaac ggatgttctg cggtggtttc cacaaacagc aggcgcgttt
    64141 cttgcggaac tttaaggccg attgccgccg cgattttcgc cgcatcgcga ccaacccagt
    64201 cacggctgac ggtgcctttt ccgcgctcgt cgatattttt cagcaacacc ggttgcagct
    64261 gctgcgcctg ttctgcggtc agtttcaccg cgtgctggcc ttccatcaga cgcatcagtt
    64321 catcggctac gctatcaaca acaatcagta ccttttcgtc ggcacaaatg atgttgttat
    64381 cgaaagacgc gcctttgacg atggactgag cagcacgggc gaggtcagcg gtttcatcca
    64441 ccactaccgg cgggttgcca gcgcctgcgg caatcagacg tttattagtg tgtttacgcg
    64501 ccgcttctac taccgcttcg ccgccggtca ccaccagcag gccgataccc ggaaacttga
    64561 acaagcgttg cgcggtttcg atatccggat ttgccacagt aaccagtaag ttttccggcc
    64621 cgcctgcggc aacaatcgcc tggttgagca gcgtgatcgc ccgctgggag acttttttcg
    64681 ccgccggatg cggggcaaaa atgacgctgt tgcccgcggc aatcaggctg atggcgttat
    64741 taattacggt tgccgccggg ttagtggacg gcgtcaccga agccaccacg ccccagggcg
    64801 cgttttcaat taaagtcagg ccgttgtcgc ccgtcagcac ttgcggagag agacactcaa
    64861 cgcctggcgt gccgcgtgcc tgagcgacgt ttttggcaaa tttatcttca acgcgaccca
    64921 tgccggtttc actgacggca agttccgcta aatctctggc gtgtttttcg cctgcttcac
    64981 gaatggcagc aatggctaac tggcgcattg ccacgctctt taacccttgc tgggcgactt
    65041 tggctgccgc aacagcgtca tccagggacg cgaaaacgcc catctcatga acggcggcgg
    65101 gcggcgtgtc actgctttgc attttcagca gtaccgcttt caccacctgt tcaatatcct
    65161 gttgattcat gatgttctgc cttatttgtg gaaaattacc tgaccgccag acaccacctc
    65221 atcgacgatg ccaatcacgc acagatcgac cggtgacgtt tcgcttttat gcgcctggcg
    65281 ggcggaactg ccactcacca gcaacaccca ctccccggtt cccgcgccaa tattgtcgat
    65341 ggcgacggca cattgcccgt caggattacc atgtggatca atcatttcca ccatcagcaa
    65401 tttgtcatgc gccagtccgt gatggcgtac ggtacaaaca atttgtccag tgacgactgc
    65461 cagtttcata cccgcctccg tggcgtattt caggtaaaag ctccccctac cctccgcgga
    65521 aggtaaaatg aaaaatgaga gagcgtgacg cccgaatcga cgtcacacag ggtgattaca
    65581 ggttgctgct atcgcctttc aggccgatcg ggaacacttc ttccaggtcg ccgtgcggac
    65641 gcgggataac gtgtacagat accagctcgc cgatacgctg tgccgctgcg gcacctgcat
    65701 cggtcgccgc tttacaggct gcaacatcac cacgaaccat ggcagtacac aggccgccgc
    65761 caatctgctt aacaccaacc agcttgacgc gcgcagcttt caccatggcg tcagaagcct
    65821 caatcagtgc aaccaggccc cgggtttcga tcattcctaa tgcttccatt gtgctttcct
    65881 ctttatcagg gtccagaacg ggaccgttca ttcaaccagt gtttgtaaac tgctttcgcg
    65941 gttcacttct gtctgacgcg gcacagctgc caccagcgcc agctcgataa tttcctgcac
    66001 gctacaacca cgagagagat cgtgcatcgg cgcggcaagt ccttgtatca atggcccgac
    66061 ggcacgatat ccgccaagtc gctgtgcgat tttgtaacca atatttccgg cttccagcga
    66121 cggaaaaacc atcacattgg ccttgccctg tagcgggctg gcaggcgctt tttgcgccgc
    66181 cacttccggc acgaaggcgg cgtcaaactg caactcgcca tcgacaacca gctttggtgc
    66241 gcgctcacgg acgatttctg tcgcctgctg gacgttggca acgcaggggt gacgggcgct
    66301 accgttactg gaaaacgaca gcatcgccac gcgcggctct tctccggtga tggcgcgcca
    66361 ggtttcggcg ctggcaagcg cgatatccgc cagctgcgcc gccgtcggct gtggtaccac
    66421 gctgcaatcg gcaaagccca acgcggggcc actgtactgt ggcagcatca ggaaaatgga
    66481 tgagagcgtt ttacagcctg gctgcaagcc gataatgcgt aatccggcac gcagcacatt
    66541 cgccgtggaa gagaggttgc ccgcgataca gacatccgct ataccggcgc tgaccattgc
    66601 ggcggcgaac atcagcggtt cggtgagttt ttccagcgca tccggcggcg ttttttcgcc
    66661 cgcgcgggcc agccagcgat gagcaaattc ttcccgcatt gcgaggttgc catgcggatc
    66721 aatcacctgt agcccgtcca tcgctacgcc atgactgagc gcaaactgac gaagttcaaa
    66781 cggattggcg accagaatgg gcgttgccag accttgttga tgtaaatatt gcgccgcttt
    66841 cagcacacgt tgatctaacg catccggaaa aaccactctg gcgggcgctc gcaacgccag
    66901 ttcacgacaa cgttcaataa tcattgcgtt tcccccagtc gttgttggat ctgcttcacc
    66961 gttagcggct gcttcgttac gctcaggatc atcatcacgt agaccgtgct cgaaaggcgg
    67021 ttcagcgcct gcaagatgtc cgggcgtaat acttcaaagc tgcgggtgat aaacacctgc
    67081 gccgctacgg tttcggtttc ccgcactttg gtgcgtagca gattgagcaa cgccgcatcg
    67141 cgcccgtggc tggcttccgg caccagatga tcgtgaccaa gataacgcaa cggctgatga
    67201 gaaagccggt gcagatcttc gtcagaaagc ccgacaatcg cctggcagcc cagcggttcc
    67261 cccagcgcat cggcgcgcat aatgttgcca agacgcgaac ggatatccgc cagccacggc
    67321 tgccacggtt ccgccagttc aatttgcagc cacactgcca gcgcaatggt gctgtcgaga
    67381 accgcgcgaa aacccagacg cggatcgctt ttagcgacca ttttttccgc cgacagatga
    67441 gtcagcgtat ccggcttttt tgccaccggc tggcggcaca gttcgcagca cgcctgcgga
    67501 tgttcatcgc tactggtcag cccgtgaacg ggctgtggct gctgctgctc atcgtcaaca
    67561 aacaggcggc cctgctcgtc aataaacttg atgcgcagat ggcggctttc cagtaactcc
    67621 cgggcagagg gcgtcaggcg actgtccgca gggagatgga tctctgcgcc ttcgctgagc
    67681 gtatggttcg ctcttagcca tgcttcggtg atgaaatctt tcatagggat tgccagttag
    67741 ccggccaggc gacatacagg aatttcacgc tggatgtcgt accaaattcg atgctggagc
    67801 ctttcgggat aaacatcaca tcgcccgctt tggcgatcat ggtttctcct gcgtggcgaa
    67861 catgcagctc cccttccagc accatatcga tttcgtcgta gttcagggtc cacgggaaga
    67921 atgcgttgtc ccactgcata aaaccggcgg ccatgctgct gccgtcgtct ccggtcacaa
    67981 ggtcagttaa gcccacgcaa tgcggctctg cgccgtcgaa acgaccaaac ttgacgctgc
    68041 taccatcaat cactttgatg ccgcctttgc cggtcaccga tttgaagctc ggctgcattg
    68101 caccctgctc cagcgactgt ttttccttca tcactttttc catcaactgc gcgaccaggc
    68161 tttcggtaaa ctggccttcc ggcagctggg caatgatggt ttcgcggatg cgctgacttt
    68221 cggttttatc ggctggcacg ctggcgggaa cggacgccgt taccggggac gattcatcac
    68281 attcggtgat ggtaaaaccc agtagatccg ccacttcacg ggcttccggg gtaataatgc
    68341 tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat
    68401 tcgctgtgat gagttttttc acctgcttcc tcctgtttgg tgagagatgc cagataatcc
    68461 accagttgct gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg ctcctcgaag
    68521 cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag catccggcat gtccgtttta
    68581 ctgatgacgg cgatttgccg tttgctgacg ccaatatcca gcaacccggc aggcaaccga
    68641 ctttccgggt cattcgcgcc gtggacatag atcagcatgt ccacatcctg tagcgtggta
    68701 attaaggcgt gataccagcg gggatggcta aaatattcac ccggcgtatc aatatcgccc
    68761 ttatcattaa attccacggc ctgtgttttt ctggcgaggg tataattccc ctgcaacaca
    68821 ttaaataacg ttgttttccc tgccccgacg gtacccacaa acgcaattcg tttcatgaat
    68881 gcctcgtatt aacttttggt catttcacaa agcgtataat ttaataagcg acctaaacca
    68941 ctgacggttt gtgataacgc ttcttctact gcccccaccg agccataaat caccagcgcg
    69001 ccgctaaaac ggtcgagaaa accgatatgc acatcggcag ctttcagcgc taaatcaccg
    69061 gcgatcatcg ccgtttcgcc aggagttagc gtcataatgc caattgcgcc cgcatccgga
    69121 acgccgattt tcttcgccag ttcctcgccg ggatgcgcga tgagatgcgc cagcgtgacc
    69181 tgtttgccgg gcacaaattc ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga
    69241 aaaacttgcg tgtatcgtga caatttttcg tcagagtaaa taacaaaatt cggcaagcgc
    69301 gtttaaaagg tgaggtagat cactaagaat ggagagatat ttactgttat gaataaactt
    69361 tcatatgcga cgcatgaata taaataaaat aatctcgaga tattttatat actgcttttt
    69421 ttattattcg caaactcagt aataagcttt actaatatat tgcagccaaa ccgcttaccc
    69481 tgaatattca gggtaagcgt gagagttaaa aaagaattac agcggttggg tttgcgcttc
    69541 aaccacggcc agcgccacca tgttgacgat acgacgcacc gatgcgatcg gcgttaacac
    69601 gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga
    69661 aacgcgcagt aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt
    69721 ggcggaacct ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc
    69781 tgcatcgccg tgcatttcac catcaatcat cagttctggt gcacgttccc tgaccagttc
    69841 cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga
    69901 caacaaagca acgcgcggtt caataccgaa acgacggaca gtttctgccg ccatcaaggt
    69961 gatctccgcc agctcttctg cgtctggttc gtcattgacg taggtatcgg caataaaggt
    70021 gttaccactc ggcagcagca gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata
    70081 accaaagaca tttttcacca cgctaaaatg ttcatgataa tcgcccaccg taccgcagat
    70141 cattgcatcg gcttccccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat
    70201 cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc
    70261 ggtccagtac tctttaaagc gcggatcgga ttcgttattg acgatctcaa aatcaacgcc
    70321 cgctttgatc tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccgat
    70381 aaggatcggt ttcgccagtc ccagcgttac cagttcctga gtggcatgca gaacgcgcgc
    70441 ctcttcccct tccggcagaa ctacgcgctt cggcgctttg cgagcctggg agaaaatcgg
    70501 cttcataaac aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa
    70561 atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc gctttcgcga ccgcaggagc
    70621 gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct
    70681 cagatcctga tcgccatacg ctgaagccac cacttcgctc tgttccgcat gggccagttc
    70741 tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg atggcggttg cgccaacgtc
    70801 cagcgcgcca cggaagatga acgggaagca caggacgttg ttcacctggt ttggatagtc
    70861 agaacgaccg gtgcagatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat
    70921 ttccggttcc gggttcgcca gcgccaggat cattggcgca cgagccattt tcttcaccat
    70981 ttcctgggtc agcactttcg ggccggaaca gccgaggaaa atatccgcgc cttcaatcac
    71041 atcatcgagg gtacgtttgc cgtcatccac caccgcatat gcggctttgg tttccgccat
    71101 gtttggctca cggccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt
    71161 ttgcagaccc agcgcgacca gcaggttcat acaggcgatt gctgcggcgc ccgcgccgga
    71221 aaccaccatc cgcacgtcag agatgttttt ctccaccacg cgcaagccgt tgaggatggc
    71281 ggcagtgctg ataattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg
    71341 ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat
    71401 gccgccgaag gtcggttcga gcgcagcgac gacttcaata aatttgtccg ggtcgagttc
    71461 atcaacttca atgtcaaata catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc
    71521 ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc
    71581 gttagagatc accgccacca ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc
    71641 tttttcgatt tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg
    71701 ctgcgttgcc agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg
    71761 gaaatcaagt gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt
    71821 tcacaaataa agtgtgtggg caacagcccc tgcccacaac gtggcgcaca ttattacctt
    71881 gccggagtct acagactttg agcaagtcca aactctcacc attaatataa tgttttggta
    71941 ataatcctat aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg
    72001 cttttttgat gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac
    72061 tttacttttc aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact
    72121 gtcgtggcag acagcggcga tattgagtcc attcgccatt atcatcccca ggatgccacc
    72181 accaatcctt cgctgttact caaggctgcc ggattatcac aatatgagca tttaatagac
    72241 gatgctatcg cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg
    72301 tgtgacaaac tggcggtcaa tttcggtgct gaaatcctca aaatcgtacc cggtcgcgtg
    72361 tcaacagaag ttgatgcacg cctctctttt gataaagaaa agagtattga gaaggcgcgc
    72421 catctggtgg acttgtatca gcaacaaggc gttgagaaat cacgcattct gatcaaactg
    72481 gcttcgacct gggaaggaat tcgcgcggca gaagagctgg aaaaagaagg tattaactgc
    72541 aacctgacgc tgctgttttc ttttgcacag gcacgggcct gtgcagaagc aggcgttttt
    72601 ctgatttcgc cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa gccgatggac
    72661 ccgtatgtgg tggaagaaga tccaggcgtt aaatcagtac gcaatatcta cgactactat
    72721 aagcaacacc actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc
    72781 ctcgccttaa ccggctgcga tcgactgact atcgcaccga atttgctgaa ggagctgcag
    72841 gaaaaagttt cgccagtggt acgtaaatta atcccacctt ctcagacgtt cccacgccca
    72901 gctcccatga gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa
    72961 aaactgtctg aaggcattcg tctgttcgcc gttgatcaac gcaaactgga agatcttctt
    73021 gccgccaaac tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc
    73081 gattcgcgca ctcagtatgg atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc
    73141 gatgggcatg gctgatattg cagaagtgct gtggaacgat tttcttaaac ataaccctac
    73201 cgacccaacc tggtatgatc gcgaccgctt tattctttcc aacggtcacg cgtcgatgct
    73261 gctctacagt ttgctacatc tgaccggtta cgacctgccg ctggaagaac tgaagaactt
    73321 ccgtcagttg cattcgaaaa cccctggcca cccggaaatc ggctatacgc ccggtgttga
    73381 aaccaccacc ggtccacttg gacaaggttt ggcgaacgcc gtcggactgg cgatagcaga
    73441 gcgtacactg gcggcgcagt ttaaccagcc ggatcatgaa attgtcgatc acttcaccta
    73501 tgtgtttatg ggcgacggct gcctgatgga aggtatttcc cacgaagtct gttcgctggc
    73561 gggcacgctg ggactgggca agctgattgg tttttacgat cacaacggta tttcgattga
    73621 tggagaaacc gaaggctggt ttaccgacga tacggcaaaa cgttttgaag cctatcactg
    73681 gcatgtgatc catgaaatcg acggtcacga tccgcaggcg gtgaaggaag cgatccttga
    73741 agcgcaaagc gtgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg
    73801 ttcgccgaat aaagcaggta aggaagaggc gcacggcgca ccgctggggg aagaagaagt
    73861 ggcgctggca cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaagatat
    73921 ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa
    73981 gtttgccgcc tataaaaagg ctcatccgca actggcagaa gagtttaccc gtcggatgag
    74041 cggtggttta ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttacaggc
    74101 aaatccggcg aaaatcgcca cccgtaaggc ttcgcaaaat acgcttaacg cttacggacc
    74161 gatgctaccg gagctgctcg gcggttcggc ggatctggct cccagcaacc tgaccatctg
    74221 gaaaggctct gtgtcgctga aggaagatcc ggcgggaaat tatattcact acggggtgcg
    74281 tgaatttggc atgaccgcta tcgccaacgg cattgcgcac cacggcggct ttgtgccgta
    74341 taccgcgaca ttcctgatgt ttgttgaata cgcccgtaat gccgcgcgga tggcggcact
    74401 gatgaaagca cggcagatta tggtttatac ccacgactca attggcctgg gcgaagatgg
    74461 tccgacgcac caggctgttg agcaactggc cagcctgcgc ttaacgccaa atttcagcac
    74521 ctggcgaccg tgcgatcagg tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca
    74581 caacggaccg acggcactga ttctctccag gcagaatctg gcccaggtgg aacgtacgcc
    74641 ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc
    74701 agatattatt ttgattgcca ccggttcaga gatggaaatc accctgcaag cggcagagaa
    74761 attagcggga gaaggtcgca atgttcgcgt agtttccctg ccctcgaccg atattttcga
    74821 cgcccaggat gaggaatata gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc
    74881 ggtggaagca ggtattgccg attactggta caagtatgtt ggtctgaaag gggcaattgt
    74941 cgggatgacg ggttacgggg aatctgctcc ggcggataag ctgttcccgt tctttggctt
    75001 taccgccgag aatattgtgg caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg
    75061 gtgatcgccg gatgctgatt gccggatgcg acgctgacgc gtcttatccg gcctacagcg
    75121 tcttatccgg cctacatgtc ccgccatttt gtttaacggg tgatccacaa cgtgggccag
    75181 gcgtctggcc catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa
    75241 cgctgaccgt caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc
    75301 gctaaggtca ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca
    75361 tttgtctcct gcccgttgtt gaacggcaaa cgcaaccgaa ccggacgcga ggctagtggc
    75421 tttttacgcg acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta
    75481 atcatcatca gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga
    75541 tcaagcgagc agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttacgt
    75601 tcttcgagtg agagtggcgt cggcgttggg ttaaccaccg cgacctcttt caacgcaggc
    75661 gctggcggta cgctgagcgg cggttcgtcc cctttcttta tccacgcggt ttcactgcca
    75721 acgcgctttt gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg
    75781 gtctgattgc cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg
    75841 gtgatggttg ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca
    75901 cttaacgcca acggctcacc atctaacagc agccgtggcg ctatctcccc ttcagacgca
    75961 tccggcgatt tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg
    76021 ctgcggctca gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag
    76081 ttttggttat tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac
    76141 accaaagacg tcggcaacaa agcgaaaaaa aagagaagga gattgcgata gcgcatggac
    76201 ggtacgaccc cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg
    76261 tggctcaatc ggatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt
    76321 ttcagtccat taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac
    76381 gtatctcgcc ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa
    76441 tatcttcatc ttcgacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga
    76501 taaaaaagtg gattagctca gtcacaccgc ctggcgacat atacagttca aataatttgc
    76561 gcacttcgcc cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg
    76621 gttcgtcgtt atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat
    76681 taacccaggt agcgacacgg aactgacgaa tcagaaccac ggtctttttc ttcgcgttgt
    76741 acaggaggat cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt
    76801 cgccatcttt gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga
    76861 gaattttgtc tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga
    76921 acaattatat tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact
    76981 gattttaccc ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg
    77041 gtaaccacca gatccgcacc acgcacggcg tcaccaccag cgaagatttt cggattggtg
    77101 gtctggtaac ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt
    77161 acaccgtgcg actccagcca cggcatcccg tgcggattga agccaaacgc cataatcacc
    77221 gcgtcggctg gcatgacaaa ttcactgcct tccaccggca ctggacgcct acgcccctgg
    77281 gcatccggct cgccaagacg cgtacgcagg aaacgaatcc cgcaaacgtg accttgttca
    77341 ttcagctcaa gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg
    77401 ttcttcacct ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg
    77461 acgttactcg cgccgtgacg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg
    77521 cccagtacca cgacgttaag tccggcggta ttgataaacg gctcttccgg tagctcttcg
    77581 agccccatca cctgtttagt gttggcgatg aggaacggca gcgcgtcata aacgcccggc
    77641 gcatcttcat tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc
    77701 gcgtcgtatt gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt
    77761 tcgaagtgaa tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc
    77821 agtttgaaag aaggaatgcc gaaagtgagc aagccaccga tttctggatg gcgatcgtac
    77881 accgtcaccg ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggacct
    77941 gcaccgataa tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa
    78001 cctttcgcca acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt taccgcaccg
    78061 tgctcatcgc gaatagtaca ggcaccttca cacaaacggt cttgcggaca aacgcgtccg
    78121 gtaatttccg gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc gatgtttccg
    78181 gctttcacca gttcaatcca ctgcggtata tggttatgca gcgggcaggt ccattcacaa
    78241 acgctgtgct cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg atcggcacgg
    78301 aatggcagat aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc
    78361 tcgccacgcg cgggcgttgc ctgcatttgc tcgactttac tcattaccgg catttcttgc
    78421 gccgcggtac tggcatgcca cggttgatgt tcctgacgtg cggtacgcaa gcggcgggat
    78481 ttcgccatgc ctgagagtgc ggcgtcagtg accagctgca gcgcgtccgc cgggcaattc
    78541 tcgacacagg caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct
    78601 ttaactttgc ccgctgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc
    78661 accacgcagg atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg
    78721 tggctgattg cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag
    78781 gtcactgcac tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatgatgt
    78841 tggctcagga catgttgctc atcattgtga gccatgacac aagcgatttc acaagcatga
    78901 caacccagac attgctgact gttggccata ataaaacgat tcataacgac cttctttttt
    78961 ggttgtaaaa accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg
    79021 gcaatgttca tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata
    79081 atcccatcac tgactaaatt gcgtttcagc gaactggaac attaatgatt ttttgtggag
    79141 aagacgcgtg tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac
    79201 attgtgctgc tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg
    79261 cgcgacgctg aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc
    79321 tacgacttgc aaagtggcag tccacaactc aatgcacatc gccagttatt tcagcaggca
    79381 ctgcattcac cggtattaac caacctcaac gtctggtatg tgccagaagc agtaaaaact
    79441 cgctatgcgc atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat
    79501 ttgccgtggt atcaggccaa tatcaataat tatgttaatc agatagacct gttcgtgctg
    79561 gctttacagc actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc
    79621 ggcatcggta ttttcacgct ggtctttttt actttgcgcc gcatacgcca tcaggtggtt
    79681 gccccgctga atcagctggt taccgccagt cagcgtattg aacacgggca gttcgactcg
    79741 ccaccgctgg ataccagcct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag
    79801 atgtcgagcg agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc
    79861 cgcgatctcc acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc gcaggcgctg
    79921 aacaccagcc agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgataat
    79981 gaagcggctg aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa
    80041 ccaaacccgg aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc
    80101 gaactgcact ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg
    80161 tcgatgctgg gacgcggttt gtactttaat caggcgcaga agcattttca gcagttattg
    80221 ttgatggaag aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt
    80281 tcttacttac gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc
    80341 gcacaaagta tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc
    80401 gagctgttga ctacttttcg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg
    80461 gaaatgctgg atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg
    80521 ccaaccctgg cactggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg
    80581 gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg
    80641 ccggacggca atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa
    80701 gaacccgaag gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctaggtggg
    80761 acgctgactt tttcacaacc ttccggcggc ggtacgttag tgagtattag ctttcgctct
    80821 gcggagggtg aggaaagtca gttaatgtaa tgcctcctac tgaccaaaga atgcttgcac
    80881 ttaaggttca gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt
    80941 ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg
    81001 aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc
    81061 aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg aacaataccc
    81121 cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac
    81181 gctggaaaac accgtgaccc aggttatcga gcaaaatatg actggcctcg ataatctcat
    81241 gtatatgtca tctcagagca gtggcaccgg tcaggcatct gtcactttaa gttttaaagc
    81301 aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg
    81361 aaagttaccg caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa
    81421 cattctgacc attgccttcg tctctaccga tggttcgatg gataaacagg atattgctga
    81481 ttatgttgcc agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga
    81541 tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt
    81601 ccagatgacg gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt
    81661 tgggcaactt ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc
    81721 ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcaaga
    81781 cggctcagag gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga
    81841 ttatcttagc cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg
    81901 cgctaacgaa atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta
    81961 tttcccgcat ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc
    82021 cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc tcgttatgta
    82081 tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt
    82141 gatgggaacc ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt
    82201 cgccatggtg ctggcgatcg gtctgctggt ggatgatgcc atcgtggtgg tggaaaacgt
    82261 cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg
    82321 gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtaccaat
    82381 ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc
    82441 ggcgatggtg ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacgct
    82501 acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa
    82561 ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg
    82621 tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct
    82681 gcgtttgccg acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca
    82741 attgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata
    82801 ttacttcacc catgaaaaag acaacatcat gtcggtgttt gccactgttg gttctggccc
    82861 tgggggtaac gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg
    82921 cgacagtaag accggcacct catttgccat tatcgagcgt gcaacaaagg cgtttaacca
    82981 aattaaagaa gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc
    83041 tgcaggtttt gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc
    83101 agcacgtaac cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca
    83161 taacggcctc gatgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc
    83221 gctgggcgtt gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta
    83281 tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc
    83341 gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat
    83401 ggtgcccttc tctgctttcg cgacctcacg ctgggagaca ggttcgccgc gtctggaacg
    83461 ctataacggt tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg
    83521 tacggcgatg gatattatgg aatcgttagt gaagcagttg ccaaacggct ttggtctgga
    83581 gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg cgctgtacgc
    83641 tatttccttg ctggtggtat tcctgtgtct ggcggcgttg tatgaaagct ggtcggtacc
    83701 gttctcggta atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg caacctggat
    83761 gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc
    83821 ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga
    83881 cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc
    83941 gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgcag gttccggtgg
    84001 tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcagccacta ttctggctat
    84061 ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga agccgcgccc
    84121 ggaataagca ataaaaaagg cgacatgcta atgtgtcgcc tttttcaact ttccgattaa
    84181 gaacctgctc agcgggttct tgctgtttgt actttgtctc aggaattact tacgaagcat
    84241 aacttcgata aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat
    84301 aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc
    84361 gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcgg tgataatttg
    84421 tatgtgatac acagcaacat ttcgagatat tcatacggca tctaatactg atttaattct
    84481 ggttaaaata cagacagata acaagatgaa tattcttaat gtttacgtta aaaatgttta
    84541 atattattta atagtt
//
LOCUS       DAZGCQ010000005        81060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000005 DAZGCQ010000000
VERSION     DAZGCQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.005"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..86)
                     /locus_tag="ACHK07_000367"
     CDS             complement(<1..86)
                     /locus_tag="ACHK07_000367"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO8100753.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKA"
     gene            complement(98..1363)
                     /gene="ydiN"
                     /locus_tag="ACHK07_000368"
     CDS             complement(98..1363)
                     /gene="ydiN"
                     /locus_tag="ACHK07_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8100754.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(1590..2804)
                     /gene="ydiM"
                     /locus_tag="ACHK07_000369"
     CDS             complement(1590..2804)
                     /gene="ydiM"
                     /locus_tag="ACHK07_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8100755.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(2904..3260)
                     /gene="ydiL"
                     /locus_tag="ACHK07_000370"
     CDS             complement(2904..3260)
                     /gene="ydiL"
                     /locus_tag="ACHK07_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO8100756.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(3396..3503)
                     /gene="rprA"
                     /locus_tag="ACHK07_000371"
     ncRNA           complement(3396..3503)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK07_000371"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(3689..4801)
                     /gene="ydiK"
                     /locus_tag="ACHK07_000372"
     CDS             complement(3689..4801)
                     /gene="ydiK"
                     /locus_tag="ACHK07_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO8100757.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            5190..8246
                     /gene="ydiJ"
                     /locus_tag="ACHK07_000373"
     CDS             5190..8246
                     /gene="ydiJ"
                     /locus_tag="ACHK07_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO8100758.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            8243..8653
                     /gene="menI"
                     /locus_tag="ACHK07_000374"
     CDS             8243..8653
                     /gene="menI"
                     /locus_tag="ACHK07_000374"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO8100759.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGYDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            8753..8941
                     /gene="ydiH"
                     /locus_tag="ACHK07_000375"
     CDS             8753..8941
                     /gene="ydiH"
                     /locus_tag="ACHK07_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO8100760.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            9490..9858
                     /gene="sufA"
                     /locus_tag="ACHK07_000376"
     CDS             9490..9858
                     /gene="sufA"
                     /locus_tag="ACHK07_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO8100761.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            9867..11354
                     /gene="sufB"
                     /locus_tag="ACHK07_000377"
     CDS             9867..11354
                     /gene="sufB"
                     /locus_tag="ACHK07_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO8100762.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            11364..12110
                     /gene="sufC"
                     /locus_tag="ACHK07_000378"
     CDS             11364..12110
                     /gene="sufC"
                     /locus_tag="ACHK07_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO8100763.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            12085..13356
                     /gene="sufD"
                     /locus_tag="ACHK07_000379"
     CDS             12085..13356
                     /gene="sufD"
                     /locus_tag="ACHK07_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO8100764.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLEQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            13353..14573
                     /gene="sufS"
                     /locus_tag="ACHK07_000380"
     CDS             13353..14573
                     /gene="sufS"
                     /locus_tag="ACHK07_000380"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO8100765.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPEDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            14586..15002
                     /gene="sufE"
                     /locus_tag="ACHK07_000381"
     CDS             14586..15002
                     /gene="sufE"
                     /locus_tag="ACHK07_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO8100766.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            15151..16155
                     /gene="ldtE"
                     /locus_tag="ACHK07_000382"
     CDS             15151..16155
                     /gene="ldtE"
                     /locus_tag="ACHK07_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO8100767.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(16219..16455)
                     /gene="lpp"
                     /locus_tag="ACHK07_000383"
     CDS             complement(16219..16455)
                     /gene="lpp"
                     /locus_tag="ACHK07_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO8100768.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(16766..18178)
                     /gene="pykF"
                     /locus_tag="ACHK07_000384"
     CDS             complement(16766..18178)
                     /gene="pykF"
                     /locus_tag="ACHK07_000384"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO8100769.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(18186..18380)
                     /locus_tag="ACHK07_000385"
     CDS             complement(18186..18380)
                     /locus_tag="ACHK07_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100770.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            18735..18944
                     /gene="fumD"
                     /locus_tag="ACHK07_000386"
     CDS             18735..18944
                     /gene="fumD"
                     /locus_tag="ACHK07_000386"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO8100771.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            19400..20026
                     /gene="ydhY"
                     /locus_tag="ACHK07_000387"
     CDS             19400..20026
                     /gene="ydhY"
                     /locus_tag="ACHK07_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO8100772.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLTGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            20047..22149
                     /gene="ydhV"
                     /locus_tag="ACHK07_000388"
     CDS             20047..22149
                     /gene="ydhV"
                     /locus_tag="ACHK07_000388"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO8100773.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            22162..22800
                     /gene="ydhW"
                     /locus_tag="ACHK07_000389"
     CDS             22162..22800
                     /gene="ydhW"
                     /locus_tag="ACHK07_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO8100774.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            22864..23532
                     /gene="ydhX"
                     /locus_tag="ACHK07_000390"
     CDS             22864..23532
                     /gene="ydhX"
                     /locus_tag="ACHK07_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8100775.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            23529..24314
                     /gene="phsC"
                     /locus_tag="ACHK07_000391"
     CDS             23529..24314
                     /gene="phsC"
                     /locus_tag="ACHK07_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO8100776.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            24318..25130
                     /gene="ydhT"
                     /locus_tag="ACHK07_000392"
     CDS             24318..25130
                     /gene="ydhT"
                     /locus_tag="ACHK07_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO8100777.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARIAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(25142..26746)
                     /gene="ydhS"
                     /locus_tag="ACHK07_000393"
     CDS             complement(25142..26746)
                     /gene="ydhS"
                     /locus_tag="ACHK07_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO8100778.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADVAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEAIRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(26872..27177)
                     /gene="ydhR"
                     /locus_tag="ACHK07_000394"
     CDS             complement(26872..27177)
                     /gene="ydhR"
                     /locus_tag="ACHK07_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO8100779.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(27285..27361)
                     /locus_tag="ACHK07_000395"
     tRNA            complement(27285..27361)
                     /locus_tag="ACHK07_000395"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27325..27327),aa:Val,seq:gac)
     gene            complement(27366..27442)
                     /locus_tag="ACHK07_000396"
     tRNA            complement(27366..27442)
                     /locus_tag="ACHK07_000396"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27406..27408),aa:Val,seq:gac)
     gene            27750..29006
                     /gene="ydhQ"
                     /locus_tag="ACHK07_000397"
     CDS             27750..29006
                     /gene="ydhQ"
                     /locus_tag="ACHK07_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8100780.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIIENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(29047..30420)
                     /gene="mdtK"
                     /locus_tag="ACHK07_000398"
     CDS             complement(29047..30420)
                     /gene="mdtK"
                     /locus_tag="ACHK07_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO8100781.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            30635..31276
                     /gene="ribE"
                     /locus_tag="ACHK07_000399"
     CDS             30635..31276
                     /gene="ribE"
                     /locus_tag="ACHK07_000399"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO8100782.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(31316..32464)
                     /gene="cfa"
                     /locus_tag="ACHK07_000400"
     CDS             complement(31316..32464)
                     /gene="cfa"
                     /locus_tag="ACHK07_000400"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO8100783.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(32755..33966)
                     /gene="punC"
                     /locus_tag="ACHK07_000401"
     CDS             complement(32755..33966)
                     /gene="punC"
                     /locus_tag="ACHK07_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO8100784.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            34079..35011
                     /gene="punR"
                     /locus_tag="ACHK07_000402"
     CDS             34079..35011
                     /gene="punR"
                     /locus_tag="ACHK07_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO8100785.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(35008..36033)
                     /gene="purR"
                     /locus_tag="ACHK07_000403"
     CDS             complement(35008..36033)
                     /gene="purR"
                     /locus_tag="ACHK07_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO8100786.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            36332..36421
                     /gene="cydH"
                     /locus_tag="ACHK07_000404"
                     /gene_synonym="ynhF"
     CDS             36332..36421
                     /gene="cydH"
                     /locus_tag="ACHK07_000404"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO8100787.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            36587..37756
                     /gene="ydhP"
                     /locus_tag="ACHK07_000405"
     CDS             36587..37756
                     /gene="ydhP"
                     /locus_tag="ACHK07_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8100788.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(37902..38483)
                     /gene="sodB"
                     /locus_tag="ACHK07_000406"
     CDS             complement(37902..38483)
                     /gene="sodB"
                     /locus_tag="ACHK07_000406"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO8100789.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(38611..39438)
                     /gene="mepH"
                     /locus_tag="ACHK07_000407"
     CDS             complement(38611..39438)
                     /gene="mepH"
                     /locus_tag="ACHK07_000407"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO8100790.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            39773..40120
                     /gene="grxD"
                     /locus_tag="ACHK07_000408"
     CDS             39773..40120
                     /gene="grxD"
                     /locus_tag="ACHK07_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO8100791.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(40171..44787)
                     /gene="lhr"
                     /locus_tag="ACHK07_000409"
     CDS             complement(40171..44787)
                     /gene="lhr"
                     /locus_tag="ACHK07_000409"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO8100792.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLSAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRATPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILSARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRTSQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(44880..45527)
                     /gene="rnt"
                     /locus_tag="ACHK07_000410"
     CDS             complement(44880..45527)
                     /gene="rnt"
                     /locus_tag="ACHK07_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO8100793.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(45630..46037)
                     /gene="gloA"
                     /locus_tag="ACHK07_000411"
     CDS             complement(45630..46037)
                     /gene="gloA"
                     /locus_tag="ACHK07_000411"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO8100794.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(46118..47215)
                     /gene="nemA"
                     /locus_tag="ACHK07_000412"
     CDS             complement(46118..47215)
                     /gene="nemA"
                     /locus_tag="ACHK07_000412"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO8100795.1"
                     /translation="MSSEKLYSPLQVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHIAVQL
                     WHTGRISHASLQPDGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRTRFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     TRLQRKAGLNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(47253..47852)
                     /gene="nemR"
                     /locus_tag="ACHK07_000413"
     CDS             complement(47253..47852)
                     /gene="nemR"
                     /locus_tag="ACHK07_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO8100796.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRNSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATSAV"
     gene            47955..48194
                     /gene="ydhL"
                     /locus_tag="ACHK07_000414"
     CDS             47955..48194
                     /gene="ydhL"
                     /locus_tag="ACHK07_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO8100797.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            48243..49139
                     /gene="ydhF"
                     /locus_tag="ACHK07_000415"
     CDS             48243..49139
                     /gene="ydhF"
                     /locus_tag="ACHK07_000415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO8100798.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            49220..49741
                     /gene="sodC"
                     /locus_tag="ACHK07_000416"
     CDS             49220..49741
                     /gene="sodC"
                     /locus_tag="ACHK07_000416"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO8100799.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(49742..51754)
                     /gene="ydhK"
                     /locus_tag="ACHK07_000417"
     CDS             complement(49742..51754)
                     /gene="ydhK"
                     /locus_tag="ACHK07_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8100800.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(51754..52611)
                     /gene="ydhJ"
                     /locus_tag="ACHK07_000418"
     CDS             complement(51754..52611)
                     /gene="ydhJ"
                     /locus_tag="ACHK07_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8100801.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVFSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(52614..52850)
                     /gene="ydhI"
                     /locus_tag="ACHK07_000419"
     CDS             complement(52614..52850)
                     /gene="ydhI"
                     /locus_tag="ACHK07_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO8100802.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            53051..53485
                     /gene="slyA"
                     /locus_tag="ACHK07_000420"
     CDS             53051..53485
                     /gene="slyA"
                     /locus_tag="ACHK07_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO8100803.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(53532..53999)
                     /gene="slyB"
                     /locus_tag="ACHK07_000421"
     CDS             complement(53532..53999)
                     /gene="slyB"
                     /locus_tag="ACHK07_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO8100804.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            54273..55382
                     /gene="anmK"
                     /locus_tag="ACHK07_000422"
     CDS             54273..55382
                     /gene="anmK"
                     /locus_tag="ACHK07_000422"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO8100805.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            55480..55809
                     /gene="mliC"
                     /locus_tag="ACHK07_000423"
     CDS             55480..55809
                     /gene="mliC"
                     /locus_tag="ACHK07_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO8100806.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSRR"
     gene            55868..56524
                     /gene="pdxH"
                     /locus_tag="ACHK07_000424"
     CDS             55868..56524
                     /gene="pdxH"
                     /locus_tag="ACHK07_000424"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO8100807.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            56653..57927
                     /gene="tyrS"
                     /locus_tag="ACHK07_000425"
     CDS             56653..57927
                     /gene="tyrS"
                     /locus_tag="ACHK07_000425"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO8100808.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            57986..58849
                     /gene="pdxY"
                     /locus_tag="ACHK07_000426"
     CDS             57986..58849
                     /gene="pdxY"
                     /locus_tag="ACHK07_000426"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO8100809.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(58893..59498)
                     /gene="gstA"
                     /locus_tag="ACHK07_000427"
     CDS             complement(58893..59498)
                     /gene="gstA"
                     /locus_tag="ACHK07_000427"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO8100810.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLASVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(59604..61106)
                     /gene="dtpA"
                     /locus_tag="ACHK07_000428"
     CDS             complement(59604..61106)
                     /gene="dtpA"
                     /locus_tag="ACHK07_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO8100811.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(61716..62351)
                     /gene="nth"
                     /locus_tag="ACHK07_000429"
     CDS             complement(61716..62351)
                     /gene="nth"
                     /locus_tag="ACHK07_000429"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO8100812.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(62351..63046)
                     /gene="rsxE"
                     /locus_tag="ACHK07_000430"
     CDS             complement(62351..63046)
                     /gene="rsxE"
                     /locus_tag="ACHK07_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO8100813.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADSLLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(63050..63670)
                     /gene="rsxG"
                     /locus_tag="ACHK07_000431"
     CDS             complement(63050..63670)
                     /gene="rsxG"
                     /locus_tag="ACHK07_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO8100814.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(63674..64732)
                     /gene="rsxD"
                     /locus_tag="ACHK07_000432"
     CDS             complement(63674..64732)
                     /gene="rsxD"
                     /locus_tag="ACHK07_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO8100815.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(64733..67051)
                     /gene="rsxC"
                     /locus_tag="ACHK07_000433"
     CDS             complement(64733..67051)
                     /gene="rsxC"
                     /locus_tag="ACHK07_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO8100816.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAISRA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQPANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVP
                     EEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(67044..67622)
                     /gene="rsxB"
                     /locus_tag="ACHK07_000434"
     CDS             complement(67044..67622)
                     /gene="rsxB"
                     /locus_tag="ACHK07_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO8100817.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(67622..68203)
                     /gene="rsxA"
                     /locus_tag="ACHK07_000435"
     CDS             complement(67622..68203)
                     /gene="rsxA"
                     /locus_tag="ACHK07_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO8100818.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(68280..68720)
                     /gene="ydgK"
                     /locus_tag="ACHK07_000436"
     CDS             complement(68280..68720)
                     /gene="ydgK"
                     /locus_tag="ACHK07_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO8100819.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(68806..69021)
                     /gene="ydgT"
                     /locus_tag="ACHK07_000437"
     CDS             complement(68806..69021)
                     /gene="ydgT"
                     /locus_tag="ACHK07_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO8100820.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(69294..69419)
                     /gene="blr"
                     /locus_tag="ACHK07_000438"
     CDS             complement(69294..69419)
                     /gene="blr"
                     /locus_tag="ACHK07_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO8100821.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            69662..70702
                     /gene="ydgJ"
                     /locus_tag="ACHK07_000439"
     CDS             69662..70702
                     /gene="ydgJ"
                     /locus_tag="ACHK07_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8100822.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKSGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(70737..71738)
                     /gene="add"
                     /locus_tag="ACHK07_000440"
     CDS             complement(70737..71738)
                     /gene="add"
                     /locus_tag="ACHK07_000440"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO8100823.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLDHGIRASIN
                     TDDPGVQGVDIIHEYNVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(71842..73014)
                     /gene="malY"
                     /locus_tag="ACHK07_000441"
     CDS             complement(71842..73014)
                     /gene="malY"
                     /locus_tag="ACHK07_000441"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO8100824.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(73024..74616)
                     /gene="malX"
                     /locus_tag="ACHK07_000442"
     CDS             complement(73024..74616)
                     /gene="malX"
                     /locus_tag="ACHK07_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO8100825.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGIVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            74791..75819
                     /gene="malI"
                     /locus_tag="ACHK07_000443"
     CDS             74791..75819
                     /gene="malI"
                     /locus_tag="ACHK07_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO8100826.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            75931..76698
                     /gene="hdhA"
                     /locus_tag="ACHK07_000444"
     CDS             75931..76698
                     /gene="hdhA"
                     /locus_tag="ACHK07_000444"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO8100827.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            76927..77517
                     /gene="uidR"
                     /locus_tag="ACHK07_000445"
     CDS             76927..77517
                     /gene="uidR"
                     /locus_tag="ACHK07_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO8100828.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            77908..79719
                     /gene="uidA"
                     /locus_tag="ACHK07_000446"
     CDS             77908..79719
                     /gene="uidA"
                     /locus_tag="ACHK07_000446"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO8100829.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNYASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            79716..>81060
                     /gene="uidB"
                     /locus_tag="ACHK07_000447"
     CDS             79716..>81060
                     /gene="uidB"
                     /locus_tag="ACHK07_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO8100830.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ"
BASE COUNT        20654 a        19861 c        21058 g        19487 t
ORIGIN      
        1 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caacccaatc
       61 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
      121 ttaaccgggt gctaaatttg cgctcgccaa accgcacatc attctcagga ataataaaaa
      181 cgcggtaata gcggataaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
      241 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
      301 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
      361 ttgggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
      421 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
      481 ctgctgttat tgttgccagt gcagagttaa atacattagc ccagatgggc cggaccattt
      541 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
      601 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
      661 acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
      721 cttccagcca gactaacggt ttgctgttca tttgcggtaa ttcattagct acgctggcgt
      781 ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt acaaacagaa
      841 tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata tagctcacca
      901 gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg accgccgaac
      961 cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc agcgctgagt
     1021 tagcgatacc tacacacact gccagaccgt aggcgagagt taaattcggg caagcgggaa
     1081 taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc cgacgaccaa
     1141 acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa ccgataccgg
     1201 aaattaagta ggcaatgccc gcgttgtcag tggaaaactt ttccgccaga gatgacatat
     1261 tttgggcaag cgtaataaca ctaatgccgt gcaggaagta gctgaagtaa atacaaagaa
     1321 cagccaggat aaatggcgtg ctgaaagcct tattttgaga cattttcaca gctccttgct
     1381 gaaagataat ttcgttaccg tacgtattgt tctccgacgg tttacataat tactatactg
     1441 aagatgattt tccctgggta catggcataa agtttatgtt tccaggaata ttgcatgtca
     1501 taatttgttt ttgatcgcga ttttccttag ttagcgcaga aagagttatt tatccgtaag
     1561 atcttgcatg acctgaaaag aatattgcat tacccacccg gagcgacatt ttcctttagc
     1621 gagtgatgcc gcgttttttt gcggctgcgt aagccgataa acagtgccag taaaaagccg
     1681 atggcagcga tggcggtatc gaaccacata atatcggcaa tacttctttg ggacagatga
     1741 gccgtaatca acggaatagt aaaggtcgca atactgcccg cactgtaata gatccctgta
     1801 gctttacctt tagcgtaggg gaaacgttca gccattaacg tcaggccaat ttgcacaaca
     1861 cctccagcag aggtaaaacc aatgacaaaa gcaaatatta tcaccacata aaatgtggga
     1921 tgcaggcaga cggtaaacag agcaataaat gagataaagg tgtacagcat cagtaatgtt
     1981 gttgggcgaa cggtattacg aatgagtgga gcggtaataa atacacaaag cagcgaaccc
     2041 acggtgtaga tactgagtag tttgatcgac atagtgtatg acatgcctgc aacaaattgt
     2101 ccgtactgtg ccagccactg gctaaccaga taaaacgttg ccattgagat atagccatat
     2161 aaggtataac tggctaaatc gataattgaa cagcgatgtt ccgtagagct ggtggttttc
     2221 tttatgacag gtaagcgacg acccggatgg ggggggaacg tacaacggta taaaaacaga
     2281 gcgttaataa acataatgcc tgcagcaatc ataaaggacc aaccgaacca cagttcagcc
     2341 cacactaaca ggctaatgat tagcggtaat aaaaattgtc cgctggaaac aaatgcttta
     2401 attaaaatat tggctgtccc aggtgagcgt ggaaaagctt ccatcaaact gggataagtg
     2461 cctgcatcga gaaaactgtt tgccattccc gccagaaagc caaaaacata agcgataatg
     2521 atgttattgg tctgcaggat gccaaaaaag aaggccatat agcagcacat cccgagcatg
     2581 ataaaagggc ggcgaccaaa gcgatcggat aataatcctg caaaaagcaa gacacttaat
     2641 cgaccaatgc ccagcgatga gataactatc gagacacccg cggcattagt ctgccaaagt
     2701 gtctccagcg aggccatatt caggctcatc aaaatgacgc ccataccatg caccaggtaa
     2761 ttaaaataca acccaagtgc ggtagggtaa tagggatttt tcataggttc tacttagcaa
     2821 aggtgcttta aaaagtgtag cgattttgtg tcagtatccg ttcattattg ttgtaacaac
     2881 gacaattcct ttttgtggag aaattaacaa agccgctcca gatcgtgtgc atataattca
     2941 gcggctaccg attgatagat cttccagtca ataaaattgc catccgggta gacacgttga
     3001 aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc cgatgcggtt atttattttc
     3061 tctataatgg catgaagatg tttttttcgt tgctgacgca tagccaacag ttgctcgact
     3121 acacgatcag gaatggcgca tttgccattt tcccactgac gccagctttc actgtcaccg
     3181 gtctgggcaa tccaggtcgc gcattcatca atagtcatgg caaaaatatg gcggagtgcc
     3241 tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa taaacaataa aatataaata
     3301 acattatcag cctgctgacg gcttgaagag agtcacagta tcttgtgcaa cattattgga
     3361 aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga
     3421 tgggcaaaga ctacacacag caattcgttg tttcactcag gggatttcca tgcttataaa
     3481 tcaatatgtt gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc
     3541 agattcgtct cattagttta aaaactgtaa agaatttgcg acaggtacta ccagctgaat
     3601 ggcggtaagc aggaaaatat ggggaagagc aagcaaatat caattagcgg tggttaacaa
     3661 tcaggcggca gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt
     3721 cttcgagaat ttcttccggt tggtccgtcg gcggcgggac ttcttccacc cacgcggcaa
     3781 acagacgcca ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag
     3841 caatcaaacc accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc
     3901 gaattaacat tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca
     3961 ataccgttcc ccaggtggta tcgccggtcc agtagagcca gataatcgcc ggaatcagta
     4021 ccggcaacgg gccaagctgg acaaggcagg agaggatcat taacaccgtt agcaacgttg
     4081 cataaggtac gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg
     4141 tcaccaccac acccagcgcc accgcgcgga tagcctgtgc cgccagcagc acggcggcat
     4201 ctccgcgaac gcctgccaga cgggtcgcaa aatggcgaat gccttgtgcc acctgttcac
     4261 cgcgccagta cagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc
     4321 cgatatgcgc cgcttgccca acgaaccagg tggtggtggt gccaatataa gggcggactt
     4381 tcgccatgat cgccgtcccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg
     4441 cgccaatcac cggaatggta ttaagccacg ccagatcggg taacgtcatg tcaccgctgg
     4501 aaatggcttt aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag
     4561 ggatgataaa caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc
     4621 caaacatgat cttttgcaaa cgtaacaata ccggccaggt ggcgataacc accgtaccgg
     4681 cccatgcaaa gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg
     4741 ctaaaaacag caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca
     4801 taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact
     4861 cacctgcata atgatgagca atttcagcgg tttttaacag gccgattctg cccttaattc
     4921 tgatgggcgt gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac
     4981 tcgtcgtatt tcaagttgca tatgctgcgg ctacgttcgt tcaccccagt tacttactgt
     5041 tgtaagctcc tggagttcac tcgcttgccg ccttcctgca actcgaaatc catagagtaa
     5101 ataaacatca cctttctgtg cgctattgca gccgcctgaa agaacagtgt atcgacctac
     5161 atcacagacc gcaggaaagg gtcaatataa tgattccaca gatttcccag gcacctggcg
     5221 tcgttcaact ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgata
     5281 cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc
     5341 tccccgatgc ggtggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg
     5401 ccgcgcagga acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta
     5461 acggtcaggc gctcaaccag ggcattattg ttgatatgtc ccgccatatg aaccgcatca
     5521 tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagaccaac
     5581 tcaatcagta cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc
     5641 gggcaacgct cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg
     5701 gcaaaacgtc agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg
     5761 atacgcaacc tttacccgtc gagctggcgg aaacgctggg taaatccaat accacaatcg
     5821 ggcgaattta taacacggtt tatcaacgtt gccgtcagca acgccagtta attatcgaca
     5881 acttccccaa acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg
     5941 agatgaccga gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta
     6001 ttaccgaagc gcggctggat attacgcgct tgcctaaagt gcggcgtctg gtgaatgtca
     6061 aatatgactc ttttgactct gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc
     6121 tttcggtaga gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc
     6181 attccgtcag cgagttgatt accgatgtgc ctgacaaaga gatgctcggg ctgaacattg
     6241 tggaatttgc tggtgatgat gaggcgctga ttgatgagcg ggtaaatgca ctctgtgcgc
     6301 ggttggatga gctgatcgcc agtcatcaag caggtgtcat cggctggcag gtgtgccgtg
     6361 agctggcggg ggttgaacgt atctatgcaa tgcgcaaaaa agccgttggt ctgcttggca
     6421 atgccaaagg tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac
     6481 atctggccga ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagttatg
     6541 gcatgtttgg tcatgtcgat gctggggtgt tgcacgtccg tccggcactg gatatgtgcg
     6601 atccgcaaca agagattttg atgaagcaaa tttctgatga cgtggtggcg ctgactgcga
     6661 aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg
     6721 cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc
     6781 ataaccgact caacccaggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga
     6841 aagtggacgc ggtgaagcgc ggtacattcg atcggcagat ccccattgcg gtacgccagc
     6901 agtggcgcgg tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta
     6961 gtccgatgtg tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg
     7021 caacgctggt gcgtgaatgg ctgcgtttgt tggcggatcg cggcgttgat ccactcaaac
     7081 tggaacaaga actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca
     7141 atagctggca tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt
     7201 cgggctgtct ggcctgtaaa gcgtgttcga cccagtgccc catcaaaatt gatgtgccgg
     7261 agtttcgctc tcgtttcctg cagctctatc acacccgtta tttacgtccg ctgcgcgacc
     7321 acctcgtcgc tacggtcgag agctacgcac cgctgatggc acgcgcgccg aaaaccttta
     7381 acttcttcat taaccagccg ctgatgcgca aactctcgga aaaacatatc ggcatggttg
     7441 atttgccgct gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa
     7501 acatgacgct ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgttgg
     7561 tggtgcagga cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc
     7621 tggtcgaaaa attaggtttc cagcctgtgt tactgccatt ttcgccaaat ggcaaagccc
     7681 agcatattaa aggttttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc
     7741 tcaaccgtat ggcgaagctg ggtatgccaa tggtaggcgt cgatccggcg ctggtacttt
     7801 gttatcgcga tgaatataaa ctggcactgg gcgaggaacg tggcgagttt aacgtcttac
     7861 tggcgaatga atggctggca agcgcacttg agtcacagcc agtggctaca gtcagcggtg
     7921 aatcatggta tttctttggt cactgtaccg aagttaccgc cttgccgggt gcgccagcac
     7981 aatgggccgc gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct
     8041 gcggcatggc agggacttac ggacatgaag cgaaaaacca taaaaattcg ctcgggatct
     8101 atgagttatc ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg
     8161 gatattcctg ccgtagtcag gtcaaacggg ttgaaggcac gggggtacgc catcctgtgc
     8221 aggctttact ggagattatt aaatgatatg gaaacgaaaa atcaccctgg aagcactgaa
     8281 tgctatgggt gaaggaaaca tggtggggtt actggatatt cgctttgaac atattggtta
     8341 tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct
     8401 acatggaggt gcatccgtgg tactggccga aagtatcggt tccgttgccg gttatttatg
     8461 taccgaaggt gagcaaaaag tggttggtct ggaaatcaat gctaaccacg tccgctcggc
     8521 acgagaaggg cgggtgcgcg gcgtatgcaa accgttgcat ctcggttcgc gtcaccaggt
     8581 ctggcagatt gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac
     8641 cgccattttg tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct
     8701 gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac
     8761 tcaacttgat cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc
     8821 acctgcccaa tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac
     8881 tctctcttct gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg
     8941 atcctgactt aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg
     9001 atgaccttct taatactgct tatctacccg cctgatattg ttcaaaaata atactaatcg
     9061 cagtttgtgt taaaacggcg ggttagcttt atgagattat tcttatcgcc ccttcaagag
     9121 ctaagccact gagagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg
     9181 tcgcacaggc ttaagggttt tcttatttct gaacgctgca cttttttacc tatctcttaa
     9241 atagtcattt tcaatgcaac tttttctttt tcatcacagc cttaatgctc cttagataat
     9301 aaccattatc taacaatgag atacctaatt cttagcgtgc ctgttaaccc acacatcagg
     9361 gtctatgctt attaaaagca ggcaaaaagc atgttttacg gtaaagcccc tgcgtttgct
     9421 gggttgaact gataatcatt atcactaaca tgctgttata cgctgaaagc gatgaagtga
     9481 ggtaaatcga tggacatgca ttcaggaacc tttaatccac aggatttcgc ctggcaaggc
     9541 ttaacgctga cacccgcagc cgctgtccac atccgcgagc tggtggcaaa gcagccgggt
     9601 atggtcggcg tgcgcttagg tgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc
     9661 gacagtgtta gcgagccgga caaagacgat ctgctgtttg aacacgacgg cgcgaagctg
     9721 tttgtcccgc tgcaagcgat gccgtttatt gatggcacgg aagtcgattt cgttcgtgaa
     9781 ggacttaatc agatattcaa atttcacaac cctaaagccc agaatgaatg tggctgtggc
     9841 gaaagctttg gggtataggc ggtactatgt ctcgtaatac tgaagcaact gacgatgtca
     9901 aaacctggac cggcggcccg ctgaattata aagaaggatt cttcacccag ttagccaccg
     9961 atgagctggc aaaggggata aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg
    10021 agccggaatg gatgctggag tttcgtctaa acgcctatcg cgcatggctg gagatggaag
    10081 aaccgcactg gttgaaagcg cactacgaca agctgaatta tcaggattac agctactact
    10141 cagcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc tgaacctggc gcggtgcagc
    10201 aaactggcgc gaacgccttt ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg
    10261 ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat tttcgactca gtttcggttg
    10321 ccactactta tcgcgaaaaa ctggcggagc agggaattat tttctgttcc tttggtgagg
    10381 cgatccacga tcacccggaa ctggtgcgta aatatctcgg caccgtggtg ccggggaatg
    10441 acaacttctt tgccgcgctt aatgcggcgg tagcctctga cggtacgttt atttatgtgc
    10501 ctaaaggcgt gcgctgcccg atggaacttt ccacctattt tcgcattaac gcggaaaaaa
    10561 ccgggcagtt tgagcgcacc attctggtgg ccgacgaaga cagctacgtc agctacattg
    10621 aaggctgttc cgctccggtg cgtgacagct atcagttgca cgcggcagtg gtggaagtca
    10681 tcatccataa aaacgccgag gtgaaatatt ccacggtaca aaactggttc cctggcgata
    10741 acaacaccgg cggtattctc aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca
    10801 gcaaaatgtc atggacgcaa tcagaaaccg ggtcagcgat tacgtggaaa tatcccagct
    10861 gcattttgcg cggcgataac tccattggtg agttttactc agtggcgctg accagcggtc
    10921 atcagcaagc ggataccggc accaagatga tccacatcgg taaaaacacc aaatcgacca
    10981 ttatctcgaa agggatctct gccggacata gtcagaacag ttatcgcggc ttagtgaaaa
    11041 tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg
    11101 ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg caataacagc gcacaactgg
    11161 agcacgaggc aacgacatca cgtattggtg aagatcaact gttttactgc ctgcaacgcg
    11221 ggatcagcga agaagacgcc atctcgatga ttgttaacgg tttctgcaaa gacgtgttct
    11281 cagaactgcc gctggaattt gccgttgaag cacaaaaact cctcgccatc agtcttgaac
    11341 acagcgtcgg ataaggaata aacatgttaa gtattaaaga tttacacgtc agcgtggaag
    11401 ataaagctat cctgcgtgga ttaagcctcg acgttcgtcc cggcgaagtt cacgccatta
    11461 tggggccaaa cggttcgggc aaaagtacct tatcggcaac gcttgccggg cgagaagatt
    11521 atgaagtgac gggcggcacg gttgagttca aaggcaaaga tttgcttgca ctgtcgccgg
    11581 aagatcgtgc aggcgaaggc atctttatgg ccttccagta tccggtggag attccaggtg
    11641 tcagtaacca gtttttcctg caaacggcac ttaatgcggt gcgcagctat cgcggccagg
    11701 aaacgctcga ccgctttgat tttcaggatt tgatggaaga gaaaatcgcg ctgttgaaga
    11761 tgccggaaga tttattgacc cgttcggtaa acgttggttt ttccggcggc gagaaaaagc
    11821 gcaacgatat tttgcaaatg gcggtgctgg aaccggggtt atgcattctt gatgaatcgg
    11881 actccgggct ggatattgac gcattaaaag tggttgccga tggcgtgaac tcgctgcgtg
    11941 acggcaagcg ctcattcatc attgttacgc actaccaacg cattctcgac tacatcaagc
    12001 ctgattacgt ccatgtgcta tatcagggac gaattgtgaa atccggcgat ttcacgttgg
    12061 tcaaacaact ggaggagcag ggttatggct ggcttaccga acagcagtaa cgcactgcaa
    12121 cagtggcatc atttgtttga agctgaaggg gtgaaacgtt ccccgcaagc acaacagcat
    12181 ttagaacaat tgctgcgtac cggactgccg acacgtaaac atgaaaactg gaaatatacg
    12241 cctctggaag ggctgaccaa tagccagttt gtcagcattg cgggggagat atccccacag
    12301 cagcgtgatg ccttagcgtt aacgttagac gccgtgcggc tggtgtttgt cgatggacgt
    12361 tacgtgcctg cgctgagcga tgcgactgaa ggcagcgggt atgaagtgag cattaacgac
    12421 gaccgtcagg ggttacccga tgctattcag gcggaagtgt ttctgcattt gacggaaagc
    12481 ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc aacggccggc aaagccattg
    12541 ctgttaatgc atatcaccca gggcgtggca ggtgaagagg tgaacactgc ccattaccga
    12601 catcatctgg aactggcgga aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt
    12661 aatgatgctc gtcattttac cggggcacgg ttcactatca acgtcgcagc gaatgcccac
    12721 ttgcagcata tcaagctggc gtttgaaaac ccgctcagtc accactttgc ccataacgat
    12781 ttgttgctgg ctgacgatgc caccgcattt agccacagtt tcctgctggg tggcgcagtg
    12841 ttacgacaca acaccagtac gcaactcaat ggcgaaaaca gcatgttgcg gatcaatagc
    12901 ctggcgatgc cggtcaaaaa cgaggtgtgt gatacccgta cctggctgga acacaataaa
    12961 ggtttttgta acagccgaca gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta
    13021 tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa cggatggtca gatgaccaac
    13081 aataatctgc tgatgggcaa actggcggaa gtggatacga aaccgcagct ggaaatctat
    13141 gcagatgatg tgaaatgcag ccacggcgcg acggtggggc gtattgatga tgaacagatg
    13201 ttctatctgc gctcgcgcgg gatcaatcag caggatgccc agcagatgat catttacgcc
    13261 tttgctgccg aactgacgga agcactgcgt gatgaggggc ttaaacagca ggtgctggcc
    13321 cgaatcggtc aacggctgcc aggaggtgca agatgacttt ttccgtcgac aaagtgcggg
    13381 ccgactttcc ggtgctttcg cgtgaggtaa acggtttgcc gctggcttat ctcgacagcg
    13441 ccgccagtgc gcagaaaccg agccaggtga ttgacgccga ggccgagttt tatcgtcatg
    13501 gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc ccaggcgacc gagaaaatgg
    13561 agaacgtgcg caagcgggca tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt
    13621 tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa tagctgggga aacagcaacg
    13681 tgcgggcggg cgataacatc atcataagtc agatggagca ccacgctaac attgttccat
    13741 ggcagatgct ttgcgcacgc gttggcgcag agctgcgtgt gatcccgctc aatcccgacg
    13801 gtacgttgca actggagatg ctacctaatc tgttcgatga gaaaactcgc ctgctggcaa
    13861 ttactcatgt ctccaacgtg cttggcacag aaaatccact ggcggaaatg atcacgcttg
    13921 cgcaccagca tggcgcaaaa gtgctggtgg atggcgctca ggcggtgatg catcatccgg
    13981 aggatgttca ggcgctggat tgcgactttt acgtgttctc cgggcataaa ctgtatggcc
    14041 ccaccggaat tggcattctt tatgtgaaag aagccttgtt gcaggagatg ccgccgtggg
    14101 aagggggcgg ttctatgatc gccaccgtca gcctgagtga aggcactacc tggaccaaag
    14161 caccatggcg gtttgaagcc ggtacaccca ataccggggg catcattggt cttggcgcgg
    14221 cgctggagta tgtttcggcg ctggggctta ataacatagc cgagtatgaa cagaatctga
    14281 tgcactacgc gctatcacag ttggaatctg taccggatct cactctctat ggcccacaaa
    14341 acaggcttgg cgttattgct tttaatctcg gtaaacacca cgcctatgat gttggcagtt
    14401 ttctcgataa ttacggcatt gctgtgcgta ccggacatca ctgcgctatg ccattaatgg
    14461 cctattacaa cgtccctgcg atgtgtcggg cgtcgctggc catgtataac acccatgaag
    14521 aagtggatcg tctggtgacc ggattgcaac gtattcaccg tctgctggga taacagggag
    14581 gcactatggc tgtattgccg gataaagaaa aattgctgcg taatttttta cgctgcgcca
    14641 actgggaaga gaaatatctc tacattattg agctgggcca gcgtctgcca gaattacgcg
    14701 acgaagacag aagcccacaa aatagcattc agggctgcca gagtcaggtg tggattgtca
    14761 tgcgccagaa tgcgcaggga attattgaat tgcagggcga cagcgatgcg gcgattgtga
    14821 aagggcttat tgcggtcgtc tttattctct acgatcagat gacgccgcag gatattgtca
    14881 atttcgatgt gcgtccgtgg tttgaaaaaa tggcgctcac ccaacatctc accccatctc
    14941 gttcacaagg tctggaagcg atgattcgcg caattcgcgc caaagccgct gcacttagct
    15001 aaactaaagg gacagctttc atccggttgg ctcacattgc cggatggtca atgtttacac
    15061 tgacctgcac aggagtgttt cggtttcagg tgaacataag cgccatccga cgtttcagcg
    15121 tgagtctccg gcaaatacag gaggtttaca atgaaacgcg cgtctttgct tacactcacg
    15181 cttatcggcg cttttagcgc catccaggct gcctgggcgg ttgattatcc gctaccacca
    15241 accggaagcc gactggttgg gcaaaatcaa acgtatacgg tgcaggaagg ggataaaaac
    15301 cttcaggcca tcgcccgacg ttttgatact gcggcaatgt tgatccttga agccaataac
    15361 actatcgccc cggtgccaaa acccggtacg acgataacta ttccttcgca actgttatta
    15421 cctgatgcgc cgcgtcaggg gattatcgtt aaccttgcag agctgcgcct ttattattat
    15481 ccgccgggag aaaatatcgt gcaggtttat ccgataggta ttggcttgca ggggctggaa
    15541 acgccggtga tggaaacgcg tgtggggcag aaaatcccta acccaacctg gacgcctacg
    15601 gcaggcattc gtcaacgttc gctggagcgt ggcattaaat taccgccagt cgttcctgcc
    15661 ggaccaaata acccgctagg acgttacgca ctgcgactcg cgcatggtaa tggcgaatac
    15721 ctcattcatg gcaccagtgc gccggacagc gtcggtttgc gcgtcagttc agggtgtatt
    15781 cgcatgaatg caccggatat taaagccttg ttctccagcg tgcggacggg aacgccggtg
    15841 aaagtgatca acgaaccggt gaaatattcc gtggagccta acgggatgcg ttatgttgaa
    15901 gtacatcgac cactatcggc agaagaacag cagaacgttc agacaatgcc atacacactg
    15961 ccagcaggct ttacgcaatt taaagacaat aaggctgtag atcagaagtt agtcgataaa
    16021 gcgttgtatc gtcgggcagg gtatccggtt gcggtgagca gtggagcaac tcccacagcc
    16081 agcaatgcgc cttcagtaga gtcagcgcag aatggtgaac cagagcaagg gaatatgtta
    16141 cgcgcgacgc agtagtggta aatagcagac aaaaaaaatg gcgcacaatg tgcgccattt
    16201 ttcacttcac aggtactatt acttgcggta tttagtagcc atgttgtcca gacgctggtt
    16261 agcacgagct gcgtcatctt tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt
    16321 gctcagctgg tcaactttag cgttcagagt ctgaacgtca gaagacagct gatcgatttt
    16381 agcgttgctg gagcaacctg ccagcagagt agaacccagg attaccgcgc ccagtaccag
    16441 tttagtagct ttcattatta ataccctcta gattgagtta atctccatgt agcgttacaa
    16501 gtattacaca aagtttttta tgttgagaat atttttttga tgggaaggca cttatttttg
    16561 atcgttcgct caaagaagca tcgaacgctg ggttttagat aaaaaaattg agagaaaaca
    16621 ggatgcttcc atcggattca tcttagataa agtgagatta tatagtgaaa tccgatttga
    16681 ttttttattg cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata
    16741 tacaaattaa ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc
    16801 cgctcggtac cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc
    16861 tctgcagagc cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa
    16921 ccagctgcgg cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg
    16981 tcagtgccag gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc
    17041 cctgggtagc aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac
    17101 ggcataccgc ttcggtaatg cgcagtttac ggttgtcatt gttgaactca agacggctgt
    17161 tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt
    17221 atttaccttt tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt
    17281 ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca
    17341 tctgggtcgc agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct
    17401 gggcgaagat aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga
    17461 tgccgtcaga ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt
    17521 tggagatgat gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa
    17581 cgtcagaacg cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa
    17641 agatcaggtc ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa acgccaggca
    17701 ggttcacacc tttgttttca cccaggtcgc cgttgttcag cactttacag ataactttgt
    17761 taccttcaat ggcagtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc
    17821 caacagacag gtcagttgtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga
    17881 taacagattt atcagtagtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac
    17941 cgccttccag tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg
    18001 ttttaccagt tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat
    18061 agtcaccatg agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta
    18121 acatctcttc agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga
    18181 cagtcttagt ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg
    18241 aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa
    18301 aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt
    18361 cgttgagttc tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata
    18421 cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta
    18481 gacaataaat tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc
    18541 tgtttgcgca acgtctggtg ccggggttgt cactgtgaag ataagtgaaa gttcatccgg
    18601 tgtataaata catcatcgat caattgctac agttaaaata atttatcgcg ttatttgtct
    18661 gattttgttt gatttttgtt taattggtta gattgatata tgctgatagt tggcatgata
    18721 aataggtata aagaatggga aatcggacaa aagaggatga actgtaccga gaaatgtgca
    18781 gagttgtcgg taaagttgtg ctggaaatgc gcgatctggg gcaggaacca aagcatattg
    18841 ttatcgcagg cgtattacgt acagcattag ccaataaacg cattcagcgc agtgagctgg
    18901 aaaagcaggc gatggaaacc gtcattaacg cactggtaaa atgaccattc aggatgaggc
    18961 gataagcttc atcctgatat tcttgcttca gttgcggtcc agttaatcat tctaaataac
    19021 aactgctgaa attatacgaa tttcgatatg tgttttaatt tataacgata atattatttt
    19081 tattttctgc aatgaccgtc acaaataatt gtcgcttaag ttaaattcat ttcaaatgat
    19141 aataaatatc agttcactta taatttcttg ctttgtaatt ttaattgata acgatcaatg
    19201 ttaagagccc agaataacca cattattttt tctcctaacc ataaaggatt agtttatcgg
    19261 cgttaaatct tttctcatac actatcgggt tagggaaaat atctggtgtt ggggatagag
    19321 tcagcagcgt attagttgaa tgcttaatat caggctgctg aattgtcagc actgtgtttg
    19381 cgaagaggat gcgaaataaa tgaacccggt tgatcgtcca ctattagata ttggcttaac
    19441 gcgattagaa ttcttgcgta tatcaggaaa aggccttact ggtctaacaa ttgcccctgc
    19501 gttactgtca cttttgggtt gcaaacagga agatattgat agcggcacag tagggttgat
    19561 aaatacaccc aaaggggtgc tggtgaccca gcgagcgcga tgcacgggtt gccaccgctg
    19621 tgaaatctct tgtaccaact tcaacgatgg ctcagtaggg acattcttct cccgtatcaa
    19681 aatccatcgc aattatttct ttggcgacaa cggggttggc tctggcggcg gcctgtatgg
    19741 cgatctcaac tataccgcgg acacctgccg tcaatgcaaa gagccgcaat gcatgaacgt
    19801 ctgcccgatt ggtgcgatta cctggcagca gaaagaaggc tgtattaccg tcgatcataa
    19861 acgttgtatt ggctgtagcg cctgtactac ggcatgcccg tggatgatgg ccaccgtaaa
    19921 taccgaaagt aaaaaatcct cgaaatgtgt gttatgcggt gaatgcgcaa acgcctgccc
    19981 aacaggggcg ttaaaaatta tcgagtggaa agatattact gtgtgaatct tgcaaaggaa
    20041 aaaatcatgg ctaacggttg gacaggtaat atattaagag tcaatctcac gacaggaaat
    20101 attaccctcg aagattccag taagtttaaa agttttgtcg gtggcatggg cttcggctac
    20161 aaaattatgt atgacgaagt accgccaggc acgaaacctt tcgatgaagc gaataaatta
    20221 gtctttgcta ccggcccatt aactggatct ggtgccccct gtagttctcg cgtaaatatc
    20281 acctcacttt ctacttttac caaaggaaat ttagtcgtcg atgcccatat ggggggcttt
    20341 tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg gaaggcgaaa
    20401 tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc cgatttctta
    20461 tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag tccggaaacc
    20521 tgtgtggcgg ctattggtca ggctggggaa aaccttgttc ctctctctgg catgttgaat
    20581 agccgtaacc acagcggtgg tgcgggaact ggcgcaataa tgggttcgaa aaacctgaaa
    20641 gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga gatgaagcgt
    20701 ctcaatgatt acatgatgac tgaacttatc ggtgcgaata acaaccatgt cgtgccaagt
    20761 acgccgcaat cgtgggcaga gtattcagat cccaagtcac gctggacagc acgtaaaggg
    20821 ctgttttggg gcgcggctga aggcggtcca attgaaacgg gtgaaattcc gccaggcaat
    20881 cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggtcc ggcggcagag
    20941 aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat gacccaaatg
    21001 aatattcctc gggtaaaaga gtttggcgtg cccagtacag gtggtaacac ttgtgtagca
    21061 aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga taaagacgat
    21121 ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg cctgtggtgt
    21181 aactacgggc agctgcatcg cgactttact tattgttaca gcaaaggtgt gttcaagcgt
    21241 gttctgccag ctgaagagta tgcggaaatt cgctgggatc aactggaagc gggtgacgtt
    21301 aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct gagccacctg
    21361 gctgatggtt catatgccat cgcagaacgc tggaatttgg gtgaagagta ctggggctac
    21421 gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa tgaagcgtca
    21481 gcgcaggtcg gttccattgt taactgtatg ttcaaccgtg actgcatgac gcatacccat
    21541 atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc gaaagaactt
    21601 tttggatctg aagatgctta cgatgaaacc aaaaactaca cgccgatcaa tgacgcaaaa
    21661 attaaatatg ctaagtggtc gctgttgcgg gtctgtttgc ataacgccgt caccctgtgc
    21721 aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg gggcgatctg
    21781 gcgcttgaag ccaaattctt caaagcgatc actggcgaag agatgactca ggaaaaatta
    21841 gatttagctg cagagcgtat ctttacattg catcgtgcct acacggtgaa actgatgcaa
    21901 accaaagata tgcgtaatga acacgatctt atctgttcct gggtattcga caaggatccg
    21961 cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat gcatgcttcg
    22021 ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgccc aacccgcgaa
    22081 acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca caatctactg
    22141 cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc gaaagtcagc
    22201 gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt tgatacggta
    22261 acggaaccag aaccggctga aatcctggca gagttcattc gccagcattc tgtggctggg
    22321 cagctggtgg cgcgcgccgt gtttctctcg ccaccctatt cggttgcaga agaggaactg
    22381 tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc ttgtatgacg
    22441 ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta cgcggcgatg
    22501 tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt tcgttttgag
    22561 tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc atattacttc
    22621 caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga atatgccgat
    22681 atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg atttatgacc
    22741 tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca gaacccctga
    22801 aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt aggggataat
    22861 cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat cttttttact
    22921 ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg gtatgccatg
    22981 attcatgacg agtcacggtg caatggttgt aatatttgcg cccgcgcatg ccgtaaaact
    23041 aaccacgtcc ccgctcaggg aagccgttta tcgatagcac atattccagt caccgataac
    23101 gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga agatgctccg
    23161 tgcatcgacg tatgcccgac aggggcgtcg tggcgtgatg agcaggggat cgtgcgggta
    23221 gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta ccaggttcgc
    23281 taccttaacc ctgtcactaa agttgcagat aaatgtgatt tctgcgctga gtcccgactg
    23341 gcaaaaggct ttccgcccat ttgcgtcagc gcctgcccgg aacacgcatt gatctttggt
    23401 cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta tcaatatcag
    23461 ctacctggtg ccgggaaacc gcatctgtat cgccggttcg gtcaacattt gattaaaaag
    23521 gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta gcgaactatg
    23581 tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga gtattgctgg
    23641 ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc gtgaagaaat
    23701 cagccaccga tcatggtgag aagatttatc tttacagcaa agcggtcaga ttatggcact
    23761 ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat cactttgcgc
    23821 tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc ggatttttgt
    23881 tactggcatg ctggctcggc tttgttctga tcaatgccgt tgggggtaat ggccaccact
    23941 atcgcattcg tcgtcagggt tggctggagc gggcggcaaa acaaacgcga ttttatttgt
    24001 ttggcattat gcagggggaa gaacatcctt tcccggcaac aacccagtct aaatttaatc
    24061 ccttacagca ggttgcctat gttggtgtca tgtatggatt gctgccgttg ttactattga
    24121 cggggctgct gtgtctctat ccgcaagccg tgggagatgt gtttcctggc gtaagatact
    24181 ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc ttcggtcatc
    24241 tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg gtcgatggct
    24301 atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg cgtatcggag
    24361 cggtgatgta tcgctggttc ctgcgccatt tcccgcgcgg cggtagttat gctgatattc
    24421 atcatgcact gattgaggaa ggatatacgg actgggcgga gagtcttgtt gagtatgcgt
    24481 ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct atgcaaaaac
    24541 tggccacaga tcccggagag agagcatttt gcagtcagtt tgcccgcagc gacgatcatg
    24601 ctcgcatcgg ctgttgtgag gataacgcca gaatcgcaac cgctgggtat gctgcacaaa
    24661 ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt cacattggca
    24721 gctccggtga gcgcgcacgg attgctgtaa ccggcaattc ttcgcggata agcagcgctg
    24781 gagatagcag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca ttgggtgaac
    24841 gatgtcatgt cgctagtaat ggtgacctgg tgcaaatcgc cagctttggc gcaaatgcca
    24901 gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat tcaacggtag
    24961 tgagcacggg cgtggtcgac tcaattattc tcgggccagg aggaagcgcc gcactggcat
    25021 atcatgacgg cgaaagagtt cgtttcgccg tcgccattga aggtgaaaac aatattcgag
    25081 ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga tagctttacc
    25141 tttaatcaaa cgaaagacgc ctacgtgcac gggaggcagg ttttacgacc gcccgagcca
    25201 tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg tcgtgcatca
    25261 accagggcaa cgcgccgaac gctacgcgcc cacgaatatc ttcgggttgc tgtaacgtat
    25321 aatcttcacc aacatcaggg atttcatccc ctgttttctg taattgttcg cgtagtccag
    25381 ggaaaggaat atctttcact ttaagcggac gctgcccgcg ggcatcaata aaaacgtcaa
    25441 acgagtagct gttgtcttcc gttttcagga cggtgcgcga ctcgttaatt tccattttgt
    25501 agtcttcacc gagagcgaga atatgaatga ttcccgcttc acgtaaggca agtaggcgac
    25561 gaatagactc tgaagggatt gccgcataat tatcgatgaa aacccgggca aggcctttac
    25621 tgaaccgttt atggtcgtgt tcattcagat gtggaacaat ttcctgtacg gcttcatgca
    25681 gacgcagaat gacataacgc cagggaacag tatgtttttc tcgtttattg cgttcgactt
    25741 cctggagatt tttttctgcc cagtcaaatg ggtcgcgttg tttgcgctcg gcaaaccagg
    25801 cttgagcaaa ggaatcgaca ttcaggctct ctaaggctat gcgttgactc cagtctggat
    25861 cagcaaactt gatttcctct actatcaatc taaatacccg atccaaaagg ccatattcgc
    25921 ctttttgaat ctcagcattt aatgcctgat cggtgacgat gtgtaagggc tcgtagggaa
    25981 tagggcaata gaaatcggct tcgggtaaaa tacccgtgcg cgacattaac gtaatattta
    26041 gcttttcact tgcgttatcg cggtgaaaaa cgacgtgttg tttatcatct tcaatgaacg
    26101 aaccatgctg aattgccact gccattgccg catccagtcc gctcaaggat gttcccataa
    26161 tacccacgtt acacgcatcg acctttgctt ccatcaggcc tgaccacggg ctgggaaaat
    26221 acgttcggat tgcttcttct tcatcaggcc agacgtgacc cgtggcaatc accgctaaat
    26281 caaacgtctc gctgggtaaa tcctgatttg tagcgagcat gacgccagca tttgtaattt
    26341 gcagatcggt aacctggcat gattcataaa cagccactgc aaatttttgc tttcgtgcct
    26401 ggtctactaa tcgtaaaaat tgatcgcgga aatattcgcc cagcagaatt cgcggtaaaa
    26461 actgacgatc gtgcaaggtt tcttttttaa cgccataacg ctggagatgg ctggcttctt
    26521 gcttttgtag ccattcgaga tacgtacaat aaatcggcgg tatttcaata ctggcaatat
    26581 ttgccagcat catttttgag ttttcctcat cactgtatgg catcccgaca ccggccacat
    26641 cagcctgctc gaagatagaa atagaaagtg gagtttgttg ctgtagaagc gagaataagg
    26701 tgtagatacc cgtaggcccg gcacccacaa tagcaatttt tttcattgtt acttacctgt
    26761 acgcattctt tcgaatttag tgttatcaga taagtttggt tgaaattttt agaatttgag
    26821 aaatttcaga cagggatgaa aagggcgcta accgcccttg attaaaatct gtcaggcgag
    26881 ttttgcctga ttgatttgac tcagtggctc attgacatcg aaaactttgg cgacaacttc
    26941 ctcaacgccg agatttttca gtcgggcagt gtgtttttcc agataggcaa gggcgctttt
    27001 ttcatcagtg aaaaggtaga tcccaccggc ttcgtggttc ttttcacttt cggtccatac
    27061 cttccacaga aaaccaggtt cctgattaat cgactcagca agcggtttaa gctgctcagc
    27121 cattgcgtcg ccaaatgggc cattgaaaga aaaatgaagt tgtaacaaag ttgccatagc
    27181 gattctcctt ctgataacgc atacgaagac agtgtaaatc ttttaacgta ttggatacag
    27241 agaagaagca gagggaaaaa ggagaagacc agattaagaa aatctggtgc gtccgagtgg
    27301 actcgaacca ccgaccccca ccatgtcaag gtggtgctct aaccaactga gctacggacg
    27361 caggatggtg cgttcaattg gactcgaacc aacgaccccc accatgtcaa ggtggtgctc
    27421 taaccaactg agctatgaac gcaacgttgt aggtgacaac ggggacgaat attagcggca
    27481 gagtgggaag gtggcaagag gcaaaacgta attttctgcg ctatttcgac cgtttgcaga
    27541 gcttttaagc aaattggcta tattttgttg atttgcaagg gcgattttta ttcaggatcg
    27601 catttacatc tgatacaacc caataattcg tgtatcagag tttaaacaga gttgtttgcc
    27661 accatctcaa atctgtcgtg gataaatcct ccgtagcctt tacacttagg tagcaataaa
    27721 ggcacacaga tgcagcaaag gaataaatca tgggatctga tgcgaaaaac ttgatgagcg
    27781 acgggaatgt gcaaattgtt aagaccggcg aggtcattgg cgcgacgcaa cttactgaag
    27841 gcgagttgat tgttgaagct ggcggaagag ccgaaaatac cgtggtcacg ggggctggct
    27901 ggttgaaagt ggcaaccggt gggatcgcca aatgcacaca gtacggtaac aatggcacgc
    27961 tatcggtcag cgacggtgcc attgccacag atattgttca gtccgaggga ggcgcaatta
    28021 gtctctctac gctcgctacg gttaatggcc gccatcccga aggtgaattc agcgttgata
    28081 aaggttatgc ctgcggtttg ttgctggaaa atggcggtaa cctgcgtgta ctggaaggac
    28141 atcgcgcgga aaaaattatt ctcgatcaag aaggcggcct gttggtcaat gggacaacct
    28201 cagcggtcgt ggtagatgaa ggtggtgaat tgttggtgta tccaggtggg gaagccagca
    28261 attgtgagat taatcagggc ggcgttttta tgctggcggg gaaagccagt gatacgttgc
    28321 ttgctggtgg caccatgaat aatctcggtg gtgaagactc tgacactatt attgagaatg
    28381 gagccatcta tcgtctgggg acagatggtc ttcagctcta cagttccggt aagacgcaaa
    28441 acctgtccgt taatgtgggt ggtcgggctg aagtgcatgc cggtacgctg gaaaatgcgg
    28501 taatacaagg tggaacagtg atcctgttgt cacccaccag cgcggacgaa aattttgtcg
    28561 tagaggaaga tcgcgcaccg gttgaactga ccgggagtgt tgcattactg gacggcgctt
    28621 caatgattat tggctatggc gcagatctgc aacaatcaac gattactgta cagcagggcg
    28681 gtgtattgat tctcgacggc agtacggtaa aaggtgacag tgtcactttc agtattggta
    28741 acatcaatct gaatggcgga aaactgtggc tgatcactgg tgcggcaacg catgtgcaac
    28801 tgaaagtgaa acgcctgcgc ggagagggag cgatttgcct gcaaaccagt gcgaaagaaa
    28861 tttcacctga cttcatcaat gtgaaagggg aagttaccgg ggatatacgc gttgagataa
    28921 cagatgccag tcggcaaact ctgtgtaatg cactgaaact acagccagac gaagacggga
    28981 ttggcgcaac gctccagcct gcgtaaaaat acaggcgcaa cccgtgaagg ttgcgccttg
    29041 tctttattag cgggacgctc gttgcagaat gatggctgac ggcatacgtt gcaggaaccg
    29101 catacgcagc atcatcataa tggctgccga cgtcaggcca ataataaagc ctatccagaa
    29161 gcctgctggc cccataggtt caacgaccag atcggtcagt gccagaatat agccgcttgg
    29221 caagcccagc acccagtaag ccgtaaaggt gatatagaaa atggaacgcg tatctttata
    29281 accacgcaaa atcccactgc caatcacctg gattgagtca gaaatctgat ataccgccgc
    29341 cagcaacatc aaatgcgcag ccagcgttac aacctcggga ttgtcgttgt acaacagggc
    29401 gatttgctcc cgcagtgaaa ccgtgaatat ggccgtcagg gttgccatac agacacccac
    29461 cataagcccg gtccgcgcag cggtttgcgc atccagcgtt gagccctgac ccagacgata
    29521 acctacgcgg atagttaccg ctgccgccag cgacattgga agcacgaaca ttagtgaact
    29581 aaagttcagg gcaatctggt gtcctgcgac atcaacaata ccgagcggag acactaacag
    29641 agccacgacg gcaaacagtg tcacttcaaa gaatagtgcc agcgcaatcg gcaaaccgag
    29701 ttgaatcagt cgtttcataa ccgcaggatc gggtttcgcg gtgccttttt cgttacgaat
    29761 atcgcgcatg gagcgggcgc gtttaatgta agaaaccatg gcaaggaaca tgacccaata
    29821 caccgccgca gtagccacgc cacaaccaac gccaccgagc tcaggcatac cgaaatgacc
    29881 ataaataaag atatagttca ccgggatgtt caccagcagg ccgataaagc ccattaccat
    29941 acccggcttg gtttttgcca gaccttcaca ctggttacgg gcaacctgga agaacagata
    30001 tcccggcgcg ccccacaaca acgcacgcag ataacccacg gctttgtccg ccagagccgg
    30061 atcgatgttt tccatggagc ggataatgta acctgcattc cacagcacca gcataatgag
    30121 aacggaaaca aaacctgcca gccagaaacc ttgtcgcacc tgatgcgcaa tgcgctcacg
    30181 tcgaccggaa ccatttaatt gcgcgataac cggcgttaat gccagcagca gtccgtgacc
    30241 aaagaggatc gccggaagcc agatagaagt accgatagcg accgccgcca tgtcggtggc
    30301 actgtagccg cccgccatca cggtatcgac aaaacccatc gcagtttggg cgatttgcgc
    30361 gagaatcacc gggattgcta atgctaataa cagacgcgct tcactgatat acttctgcac
    30421 gtgaacacct tttatttgta gttatatgag agactaaaaa gccgcctgaa tgggcagcaa
    30481 aaagaagaag caggggaaat tcagtctatt gtagcgagga tttattaatt ctccagtgaa
    30541 aaaatagtca ttcttacccc ttcactggta agggcgtttg ccacctgcta ttgtgctgaa
    30601 cagaatgtct taactgattt caggagttgt aagtatgttt acggggattg tacagggcac
    30661 cgcaaaactg gtgtcgattg acgagaaacc aaattttcgt acgcatgtgg tggagttacc
    30721 cgaccacatg ctggacggcc tggaaaccgg tgcgtccgtg gcgcataacg gttgctgcct
    30781 gaccgtgacg gaaattaacg gcaaccatgt cagttttgac ctgatgaaag aaacgttacg
    30841 cattaccaat cttggcgatt taaaagtggg ggattgggta aacgttgagc gtgcggcgaa
    30901 attcagtgat gaaattggcg gacacttaat gtcaggtcat attatgacca ccgctgaagt
    30961 ggcgaaaata ttaacctcgg aaaataatcg ccagatctgg tttaaagtcc aggatagtca
    31021 gttgatgaaa tatatcctgt acaaaggatt tattggcatc gacggtatta gcctgaccgt
    31081 cggcgaagtc acgccaacgc gtttttgcgt ccatttaatt ccggaaacac tggaacgcac
    31141 gactcttggg aagaaaaaac ttggcgcacg cgtcaacatt gaaatcgatc cacaaactca
    31201 ggcagtggta gatacggtag aacgtgtgct ggcggcacga gaaaatgcca tgaatcaacc
    31261 aggtacagaa gcctgatctg aaatcgcccc cggagcgggg gcgatagaat agcctttagc
    31321 gagcgactcg aaggccgttt tcaacaccgc gtgagaacac cacctgccag agctgaatat
    31381 cacgggcgcg gaaagcacct gcgcaggcat tcagataata ggtaaacatt cgtttaaagc
    31441 gttcactgta gttatccgca atttctggcc atgcagcgag gaatcgttca taccacgcca
    31501 tcaacgtagt atcgtaatca gcaccgaagt tatgccagtc ttccatcaca aagtggggtt
    31561 cgctggattg agcaatctgg cgtacagagg gcaggcaacc gttcggaaaa atatatttgt
    31621 taatccaggg atcaacattc agatcggttt ttttcgaacc gatagtatgg agcaggaata
    31681 taccttccgg tttcaaatta cgatccacca ccgcaaaata ggtatcgtaa tttttcggtc
    31741 cgacgtgctc gaacatcccc acagaaacaa tacgatcaaa ctggtcgttc aggtcacgat
    31801 aatcttgcag caaaatggtg acatccaggc cttcacagcg ttcctgagcc attttttgct
    31861 gttcggcaga aatggtgacg ccaaccacgc ttacgtcata attagacgcc atgtagtgcg
    31921 ccagtccgcc ccagccgcag ccaatatcca gtacgcgcat ccctggtttt aactgcaatt
    31981 tttcacaaat cattttgagc ttcgcctgct gggcagattc cagattatcg gcatctttcc
    32041 agtaagcgca ggaatattgc atgaagggat caagcatgcg gctgaacaag tcattaccca
    32101 aatcataatg ctctttgccg actatccagg cacgtttttt actctgcaga ttgaagagac
    32161 gagcgccggc aatacgcagc gtgtctttga aatgatgggg gagttggttc tcgagacctg
    32221 cgcgtaagac tttgctaaaa aacatatcca gtcggtcaca ttcccaccag ccatccatat
    32281 aactttcgcc taaccccaaa gagccttctt gcagaacgcg tttaaaaaaa tcggggtttt
    32341 tcacacgaat atccgccggg gcagaaccgt taatggctat accggcacgg ctaagtaatt
    32401 cgttggcgat acggtaccag ttgtcatccg gtacactgac ttcttctata cacgatgaac
    32461 tcatagtttc tccatcactg gtcggtgatc agaaccttaa aacagcgtag acgctttttg
    32521 ggctttgtga gaaatctcgc agaaaaaacc gcgagtctat aatccgacgt agaacagagg
    32581 gaaaggagga acccttgccg aaatgaccat ccatagtgag tagggaacga tcctgtcccc
    32641 gtaaaaacat aatatttatc gtagctttaa caactcgaaa ttagtatagg ccgctaattt
    32701 caatcataca acaaaatttt gttagcagcc taagtataag tatatcgata tagatcagtg
    32761 tgattcgcta tgagcgactt cggcattgcc atgattctgg cagccaactt cttcacaacg
    32821 ttgcatcatg taacccagcg cgaccagcac tactgttgat aacatcacgc tggtggtggt
    32881 gagcaatggc gtgctgatac tgatcagcca ggaaactacc agacttgcga ggaagcacag
    32941 acccagttgt agagtgttct gcaacgctgc ggcgcgacca gttgcgtgtg ggaagggacg
    33001 cagcgcctgg gcgacaacaa tagggtagat cgcgccattg gcaatcgcca tcacacagaa
    33061 tgggatcagg atttcgacca gcgacacatg gctaatgaag cccgcagccc aggtcgcaat
    33121 gacgctgaca gcaaacagca ccagcaacca cggtaataac tgcttgcctt gccatttctg
    33181 cagcgcggcg cgacagccat aaccaccaat cagaaacgcg atagtttgcg ggacataact
    33241 taaaccaata actgccgggc tgtagcccat ttcactaagg atgaacggtg aaccggtcag
    33301 ccatgcaaaa aaactggctg aacaggccgc gtatatcagc acgttgccgc gataggtttt
    33361 agaacgtagc aggtcggtaa aggtcagacc atcctgacta ttgttacggg ccttcgtcgt
    33421 gggtttgagc cagaaaatag gcagaatcag caccacggta atggcaaaaa gggtggcgaa
    33481 aatcgcctgc caggaaaaat ggaccagcag ccagcttcct aacagaggag ccagggccgg
    33541 agatagaccc accagcggca tgatggtcgc aaaaattcgg ttaactttct gtgaaggata
    33601 ataatccgtc actaacgctt gccagataac cgccgcggcg cagacaccca cagcttgtac
    33661 aaaacgcaat accagcagca tagcggcgtt ttctacccac agcatcccca gactacctaa
    33721 cgcaaaaatt gtcaggccga ttaataatac cggtttacga ccataacggt cggaaagcgg
    33781 cccccacaga agctgggctg cggcaaaacc ggcaaggaac agactaaggc tggcactaac
    33841 agcagacgca ggcgtttgca gatcggcctg tatggcggcg aaagcaggca ggtacatatc
    33901 ggttgccaga aaaccgagta cgctcaaacc cgccagccag actaaaaatc ttttcccagg
    33961 ttgcatgtat attctctaaa tctctcaatt aaacaccgcc gcaaagtgta gggagtgaaa
    34021 ttagtgttgt gaaacggtaa tatttgatga ctggtttcaa aaattttgca ggcagactat
    34081 gtggtcagaa tattcactcg aagttgttga tgcggtagcg cgtaatggta gttttagtgc
    34141 cgcagcccag gagttgcatc gcgttccttc tgcggtcagc tataccgtac gtcagctgga
    34201 agagtggctg gcggtgccgc tgtttgaacg acgtcaccgt gatgtggaac tgaccgctgc
    34261 cggggcgtgg tttctcaaag aaggtcgctc tgttgtcaaa aaaatgcaga tcacccgcca
    34321 gcaatgtcag cagatagcga acggctggcg tggtcagtta gctattgcag tggataatat
    34381 tgtcaggcca gaacgtacac ggcagatgat cgttgatttt tatcgccatt ttgatgatgt
    34441 cgaacttctt gtctttcagg aagtgttcaa cggtgtctgg gatgcgcttt ccgacgggcg
    34501 cgtggaactg gcgattggcg caacacgcgc gattccggta ggcggtcgtt atgccttccg
    34561 ggatatgggg atgctaagct ggagttgcgt tgttgccagc catcacccgc tggcgttgat
    34621 ggatggcccg ttcagcgatg atacgttgcg caactggccg tcgttggtgc gcgaagacac
    34681 ctcgcgaacg ctacctaagc gtattacctg gttgctggat aaccaaaaaa gacttgtcgt
    34741 gcctgactgg gaatcatcgg caacctgtat ctcggcagga ttatgcatag ggatggtgcc
    34801 aacacatttc gccaaaccgt ggcttaatga ggggaagtgg gtagcattag aactggaaaa
    34861 cccctttccg gattcggcat gttgtctgac atggcagcaa aatgatatgt cgcctgcgtt
    34921 aacctggttg cttgagtatc tcggcgatag tgagacgctg aataaggagt ggctgcggga
    34981 gccggaagag actcccgcaa cgggtgatta acgacgatag tcgcggaacg ggccgtcagc
    35041 cacggagcgg cgttcaatca agcgcggatg cacttcaatg gactgcggtt cttcacgttt
    35101 gttgacgata cgatccaaca gcatgttgaa cgctgtttca cccagcgagt cttttggctg
    35161 atggatcgtg gtcagcgccg gcgtaaaata gcgggcgttg cgcacgttat cataaccgat
    35221 cagcgaaaca tcctgcggga cgcgcagccc catttcatca gcagcacaaa gtgcgcccat
    35281 tgccatgata tcgccaccac agaagacggc agtagggcga tgcggctgcg acaggatttg
    35341 ctgcatggcg cgataaccgg attcaggttc aaaatcaccc tgcacaatcc agctttccgg
    35401 caccttgatc atcgcttctt ccatcgcctt cataaaaccg gcaaggcggc ctgcgccggt
    35461 gttacgttcc agcgggccgg ggataacgcc gatttcgcga tggccgcgtt ctatcagata
    35521 acgcccggcc atgtagccgc cttcgaacgc gttatcaatg accgcatcgg tgaagtcagc
    35581 ttttgcttca ccccagtcca tcaccaccat tgggatatgg cgatactctt ccagcatcgc
    35641 cagcaacggc tctgggtact cagaacacat taccagcaga ccatcgacgc gtttttgcgc
    35701 catcatcgac agataagccc gctgtttctc aagattattc cacgcattgc ccagaatcag
    35761 ggtgtaacct ttctggaagc aatttttttc aactgcttca atgatctcgg caaaataggc
    35821 cgcttcgctg ctggtcgcca gcaaaccgat agacttggtg tggttaacct tcaggctacg
    35881 cgccaccgcg ctaggggagt agtgtaattc tttaatcgct gcccacacgg cgttgcgcgt
    35941 ttcttcagcg acgaaacgtg ttttgttgat cacgtgtgac acagttgtag tggaaacgtt
    36001 tgctcgtttc gctacatctt ttattgttgc cattccattt cactccagac cctaacttca
    36061 gctcctgcaa aatcgcaagg taaacgtttg cctttacaca ccttacgcac aacaaaagtg
    36121 ttgcggtacg ccggaaaaac gacacggagc gtcaggaagg ggtcaatggc cagtacgcaa
    36181 atagtgtaag cgtggaattt tggccgatct tgacgaaaag gggaagtggt aaaaacactt
    36241 atcattataa atgacgggag attttttcat cacagtgtgt aaaaatgcgt aatatcactt
    36301 tttgtgtggt aataaaaaag gagaaaactt gatgagcacc gaccttaaat tttcactggt
    36361 aacaacgatt atcgtcctcg gtttgatcgt agccgtgggt ttgactgccg cgctgcactg
    36421 atttcctctc gagggagcgc gttctccctc gcatctcctt cctgtttgtt agcaaaatgg
    36481 caaaagtgtt ttctcttttt gttattttca tttttttgtg attgctgtca tgctttcgcc
    36541 actttaatga atatcgcgta atgacgcgaa gaggtggaga taaggcatga aaattaacta
    36601 tccgttgctg gcgctggcga ttggcgcgtt tggtatcggg acaacggagt tctcgccaat
    36661 gggcttgttg cccgtcattg cgcgcggtgt ggatgtctcg attcccgctg ccggaatgtt
    36721 aatcagtgcc tatgcagttg gcgtaatggt tggcgcgccg ctgatgacgc ttctactttc
    36781 tcatcgtgcc cgccgcagtg cgttgatttt cctgatggca attttcacgc tcggcaacgt
    36841 actttccgcc atcgcgccgg attatatgac cctgatgctt tcacgcattt tgaccagcct
    36901 gaatcatgga gcattttttg gtttgggttc agtcgtggct gcaagcgtgg tgccaaaaca
    36961 taaacaggcc agcgcagttg ccactatgtt tatggggtta accctggcaa atatcggtgg
    37021 cgtgccggcg gcgacctggt tgggtgaaac catcggctgg cggatgtcat ttctggcaac
    37081 ggcggggctg ggagtgattt caatggtaag tctgttcttc tcattaccta aaggtggtgc
    37141 aggggcacga cctgaagtga aaaaagagct ggcggtatta atgcgtccgc aggtgctgtc
    37201 tgcattgctg acgacggtac tgggagctgg tgcaatgttt actctctaca cctatatctc
    37261 tccggtactg caaagtatta cccacgcaac accggtgttc gtcacggcaa tgctggtgct
    37321 gattggtgtc ggattctcta tcggtaacta tctcggcggc aaactggcag atcgttcagt
    37381 taacggcacg ttgaaaggct ttttgttgct gctgatggtg attatgctgg caatcccgtt
    37441 cctggcccgc aatgagttcg gcgcagctat tagcatggtg gtgtggggcg ctgcaacctt
    37501 tgcggtcgta ccgccgttac agatgcgcgt gatgcgtgtc gccagtgaag cgccaggtct
    37561 gtcttcatca gtcaatattg gtgcctttaa tcttggaaat gcgctgggag cagccgctgg
    37621 tggtgcggta atttccgctg ggctgggata cagctttgtg ccggtgatgg gggcgattgt
    37681 cgcgggactg gcattattgc tggtgtttat gtcagccaga aaacaacctg aaacagtttg
    37741 cgttgctaac agctaattaa acaaaaacgc agaaggataa ccttctgcgt tacagttgct
    37801 gacaacgtgc gatttatctt gccgaatgcg ctgttaacgc ctcatccgcg ttggcatggt
    37861 aagcgaagcg catcaggcaa tgctgcattt gccatcagtt attatgcagc gagatttttc
    37921 gctacgaatt cccagttcac cagcgcccag aagtgctcca gatagccagg acgtgcattg
    37981 cgataatcga tgtaatatgc gtgttcccag acatcaacgg tcagcagcgg agtcgcatcg
    38041 gtggtcagtg gagtacccgc gttagaggtt gaaacgatag ccagtttgcc atcgctgttt
    38101 ttcaccagcc aggtccagcc agaaccaaag tttttgatcg ctgcatcagt aaactgagct
    38161 ttgaaatcgg caaagctgcc aaaagatgcg gcgatagctt cagcgacttt tccagtcggt
    38221 tcgccaccgg cgttcggtgc caggcagttc cagtagaaag tatggttcca gacctgagct
    38281 gcgttgttga atacgccacc ttcagagctg cgaataatct cttccagtga tttaccttca
    38341 aacgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg atgcttgccg
    38401 tagtgatact cgatagtttc cgcagaaatg tgcggtgcca gagcatcttt agcatatggt
    38461 agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc atacgtacaa
    38521 tagccttatt gtgcgtatgg atagggtagc aaattaaaag gtgagacaaa agatgaactt
    38581 accctgttgc cgtaacaaca gggtaaagtt ttagcgaagt gtttttgggg tcattacccg
    38641 acgagcgcca acatagtggc gctgccagta gtcttcactg agagaagtga tttgaatttc
    38701 ctgacccgta cgcggtgact gaataaattt gccgttgccg acatacacac cgacatgatc
    38761 ggctgtaccg cgtccctgag tacggaaaaa gaccaggtcg ccgtttttca gttcactacg
    38821 ttcgattggc gctgcatcgc gcaggtgata catttcattc gccgtacgcg gaatacgaat
    38881 ttttaccaaa tctttataag cgtaataaac caggccgctg caatcaaaac cggtacgcgg
    38941 tgagctgcca ccccaacgat atggcttacc aatttgctgc atcagtttat tcattgccac
    39001 tttcgtagct ttttgcacct tcgctttgtg cgcgtcggca agagttcctg acgcggcatt
    39061 tttgactttc acacaatgcg atttatagcc cttacgtttg gtacactttt cggtaaagct
    39121 ggctgaggcc gttttattga cggcgtgttt gctgcgagtt ttggcgtttt tagaagcggt
    39181 aatggaagat tttttaacgg aagaagcggt tttgcttttg gtcgtacttt tactggcggc
    39241 ttttttcgct gttttctggc tggctttctg ggtctttttg gtggttgccg tgcttttctt
    39301 tttatctgct tttttggtga tatgagtggt agcagaactc tccctggctt gctttgaagc
    39361 atgggccata ggcgttaaag gcagggtggt aaaaagtaaa gcacagagcg tgatcgaaat
    39421 acggtttatc cgcgccactg agcgtccctc tgttaaggca ggtctatcat gcctgcgtct
    39481 gatttacaaa acttgatgat tctaatgcat agaagaagag aaagttaata gcttttattg
    39541 ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag cagtgaaagc taaaaatggt
    39601 tataaatacg ccagtttgcc gcgcatcaga gaagaaacga atatttgaaa tgcaacaaat
    39661 agagcaaagg gataaaatag ctaaagcgtg atgaaaatgt tattttccga tgctgcactg
    39721 acccgctaca atgtcagatt actgccgtaa agaagtttga ggaagcaaga caatgagcac
    39781 cactatcgaa aaaatccaac gccagattgc tgaaaacccg atcctgctgt acatgaaagg
    39841 ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg
    39901 tggcgaacgt tttgcctatg ttgatattct gcagaatccg gacattcgtg cggaactgcc
    39961 gaaatatgct aactggccga ccttcccgca actgtgggtt gacggcgagc tggtcggcgg
    40021 ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga tcaaagaaac
    40081 tgccgctaaa tacaagtctg aagagccgga cgcggaataa tccgtcctgc aatactgacg
    40141 gatgcgaacg ggcatccgtc agtcctttct ctatccccaa tccagccctt gtggcgaact
    40201 tgaaaatccc gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg gtagatcatt
    40261 cacttctgtt agcgtaaagc gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg
    40321 gaaaacctct ggggcgagta attcctcttt ttcctgccag accagcattt ttttgccacc
    40381 ttgcgccaga tagagtaaca atttgccgcc agaaacgacc accagcgcac ccgcccgacg
    40441 cgttggtacc agcgttgccg gatgtgcagg ccagggcaga agatttcccc acacattagc
    40501 cggatcgttg gcggagagcg ccactggtgt atagtggcgc gtttgcgcgg cttgtgtcgc
    40561 caggtcgcgc aagcggtcaa tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc
    40621 tacaaaacga cctcgcataa ttcgcccgga atcttccata cttcgacaaa gcgtttgcat
    40681 cgatggaaac ccgccaggga tattttcggc tatcaccgcc tgacgactga tgatgccgta
    40741 gcggtcgagc atattttccg ccagcgccag catcctttcg gtatcgttta gtggctccac
    40801 ctgcaataac gaccagcgtc cagccagatt tggtgtgttg taagataccc gcggcgagac
    40861 gggttgtgca tagacaggac gcccacgacg agcccggtga ctgcggcgag ttgaggtgcg
    40921 tgcgttggaa ctgctgcggg tgagggcgcg taacggtgcc caaatatcgc tggtgatgac
    40981 gccttgccag accagcgccc ataacgcctc ttgcagtgcc gagggatcaa ccgattcgcc
    41041 gattttgtcg cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc cgtcagccag
    41101 aacagcgact atcgcttgtt gcagcgccga acgattcgcc tgatccgctt cagcgggagt
    41161 gaacgattct gcagcatatt cctgtagatg cagtgccacc aggccgtcat cttcacccag
    41221 ttttttttgc cccgaccaga taaccgcacc ggttgccagt aattcatcga gcatttctgg
    41281 cgaatagtcg cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc
    41341 gactccggca agctgttcga tcacccgcat cacgccatcc acgccctcat aaacgcctgg
    41401 cgatgttgag gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc
    41461 cagcagcaat cgcgcatagg tcgtggccgc aacgggacgc gtcgcttctc tggcggcttg
    41521 cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag
    41581 attcatcacc agaccctgtt cgcgcagttg ctgaagctgc tcttcgacaa tggcaatacc
    41641 gagactaaac tcatgagcca gttgttcagc cgtgaccaga gcatgagccc ggagatagcg
    41701 cagaaagagg tcgcgaagcg ggtaactgac tctatgcaaa taaatctctg gcagagactc
    41761 tggtagttgt acgccgaggg catcgcgcag cctggcggca tcatccatac aggcaagacg
    41821 ctcctgtccg ctaatcatcg ccgggaagat gcgttttact gtaagaagat tttccagata
    41881 cgacgcaatc tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa cggtcattgg
    41941 ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc
    42001 cagtcgttgc aactcttctt ccacctggcg aatgacctgc gggtcgagta attcccccgg
    42061 atcgacctgc ccgagtagat tgcgcagtaa ctcgctgtcc agtgacagta cggatgcccg
    42121 gcgctctgcc agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa
    42181 actggtggcg aaaggcgagg gcgtagtagt cgttacatcg gatatttgaa tttcgccacc
    42241 gttcaggcga cgcatcaaac gttccagtgc gggaagatca taaacatctt gcagacattc
    42301 gcgtagggtt tcgagaatga ccggaaaatc cggatatccc tgagcgattt ccagcaactg
    42361 actggtgcgc agccgttgtt gccagagcgg ggtgcgatgg cccggcgtgc ggcccggcat
    42421 taataacgcc cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgctg agctgcctac
    42481 cgcctcgcgg acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcgtcggg
    42541 caatttacca tcagtgtcag gaatgcgcgc aacaatgccg tcatcactgg cgaccacaga
    42601 cgcgtcagcg ccccatagcg catgtattcg cccggcaatc gccagcgccc agggttcatg
    42661 cacccgtctt ccatagggag agtgcaaaat aatacgccag tcgccaattt catcacggca
    42721 ccgttcgagg accagatgac gactccccgg aacaataccc gtcgcgttgc gctgctcgtc
    42781 aatcaacccc tgaatattgg cgatcgtatt ttcttctgcc agccacgggg gaatagtccc
    42841 ggaaaagaac gcgccatccg ccagtaaatg aagaaaatcg ccgatcatct cgcctaattc
    42901 agccggacgt ccgttacctt caccacgcca aaagggaagc cgggcagaac gacccggagc
    42961 aggagtcaca atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag
    43021 agtgataata tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg
    43081 ccgcgaaccg gcctgttctt caccttcggg taataacacg ctatacatgc cacgatccgg
    43141 aatggtgcca ccgctggtaa ccgccaacag ctgagcgcca ggtcgggcgg acaatatccc
    43201 ggtctcccgg ttccagacca gttttggtcg aaaagcagaa aaatcgccag agggatagcg
    43261 cccggaaagc atatccagcg tggcgtcaaa aacacgtctt ggcagatctt tccacggtgt
    43321 ggcacggcgt acgcgggagt accattcgtc tacctgtaat gcatccatcg ccgcggcggc
    43381 aacggtgtgc tgcgcaagga cgtcgagtgg attatgcggt ggtgtcaggt tttccagcct
    43441 gcctgcgaac atacactcta caatgactgc ggaatcgact aagtcacgcc gggttcgggg
    43501 gaaaaacagc cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa
    43561 cccactggca acagaaagcg gcgttgccac ttgaatcacc agatccacag cgcccatatc
    43621 aatccccagt tcgagactgg aggttgcgac cacgcagcgt aattcacccg atttcagcgc
    43681 ctgttcggtg attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa
    43741 aatgtcgcta ctttgcacac ggttagaggt tgcgccggag gtcgactcga aatgggccac
    43801 atcaacggcg atagaagggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc
    43861 cgtcagtttt tccgccagcc cccgcgaatt agtaaagaca atggtcgagc gatggcgcaa
    43921 cacttcatca aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg
    43981 gctgtcttcg ccagtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac
    44041 gacaatccgt atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc
    44101 gccgccaaga aatgctgcca catcgctggc tgagcgcaca gtggcagaaa ggccaattcg
    44161 ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc
    44221 acgtttactg cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag
    44281 cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat
    44341 atccggtgga ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat
    44401 ccccacgcga agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt
    44461 caacgggatc tgcaagttgc gctgaacgtc ggtgcccagg gcttttatcg gtgaaatata
    44521 gagaatgcgt gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa
    44581 gagccgatcg agggcgtaga gaaatgctgc cagcgttttc ccggagccgg tcggtgcaat
    44641 caccagcgca tgttcgcctc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg
    44701 ctgtttaaag gcgctaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcagggg
    44761 cgatgaaagg tttggattat ctgccattaa cgctattccc cgtgaatatc atgcatgatg
    44821 cgtggaatag cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat
    44881 tacacctctt cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt
    44941 tcacaaaaca gcaccgcagt gcgttcggtg tcgtacagcg cggagtgagc ttgggtgctg
    45001 tcgaagtcca tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc
    45061 ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagtgag
    45121 gcgcgttcag cggcggccat cataaagctg tgatcgaaat tggcgttgtg cgccaccata
    45181 atggcgcggt tacagccgct cgctttaata cctttacgta caactttaaa aatttcgtgc
    45241 agcgcctcgt attcgctgac cgcgccgcga tcgggatcat tcgggtcaat gccgttgaag
    45301 gcaagggctt ctggttgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg
    45361 gtgtccggca tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc
    45421 agcgcatcgg ttttggcgtt aaatccggct gtttcaacat cgatcacaac aggataaaaa
    45481 ccacgaaaac ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa
    45541 tacggataaa ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg
    45601 ggcgcgatga gttcgcgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttc
    45661 tcttcgatta actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta
    45721 ccgcctttta ccggacccgc ttcacgggtc acgttacccc cgttttgacg gattttttcg
    45781 cacgcttcag cggcattatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg
    45841 tatttatcca cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg
    45901 tagccaacaa acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc
    45961 atgcccagaa ctttggtata aaaatcgatg gagcgttgca aatcgccaac gcgtagcatg
    46021 gtatgaagaa gacgcatctt tatatcctca aaatggctaa cagattcaaa atgttgtttt
    46081 agtatagcgg cggctttgcg ccgctcgcaa tgttggatta cagcgtcggg taatcagtgt
    46141 agccttccgc accaccgcca tagaagcttt cagcacgctg tgggttaagt ccggctttgc
    46201 gctgcaagcg ggtaaccaga tcagggttcg caatccagtc acgaccaaat gccaccgcat
    46261 caattaaccc tttgccgatc agcgtttcag ctttttctac tgtgtatgca cctgcgccga
    46321 taatcggacc gtggaaacgg gtgcgtactt tttcgcggaa cgcatcagta tacggttcac
    46381 ctcccgccca atccggttct gacatatgca gataagcaat gccgcgttta cccagttgtt
    46441 caatcaggta cagcgcatcg gcttcttcat ttgggccatt atccgtgttc tggaaagtac
    46501 cgattggcga aacgcgaatg ccaatgcgat cggcacccca ttcttcaatg cctgcatcga
    46561 ccacttccag caccagacgt gcgcgatttt ccacgctacc gccgtactga tcggtacgat
    46621 ggttcgaaga aggcgaaaga aactgatgca gcaaataacc gtgagcagag tggagctcga
    46681 ccagatcaaa accggcttcg cgggcgttgg ctatggcctg gcggaaatcg ttgacgatac
    46741 caggaatctc ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac
    46801 cattttcatc gcgcagagaa gtgcgtgttc ccgcgctaag tgctgaaggt gctaccggtg
    46861 cctgaccgtc aggttgcagg ctggcgtgag aaatacgtcc ggtatgccac agttgcacgg
    46921 caatatgacc attttcagca tgaacgccag cggtgatttt tttccacgcg gcaatttgct
    46981 cctggctatg gatgccaggc gcaccagcat acccttttgc ctgggcagaa atttgcgtgg
    47041 cttcgctaat aatcaaacca gcactggcac gttggcgata gtattccgcc atcaacgggg
    47101 tagggatgtc acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt
    47161 ttgccgccgt gatcgcgccc acttgcagtg gggaatacag tttttcagat gacataaagg
    47221 ctcccggtcg tctggtgata aaaaaatgcc tgctaaacgg cagacgtcgc aataatggtt
    47281 tttacatgtg ccagcgcgtt ttcaagtggc tcgaaactgc gcgaaatttt ggcctgcaga
    47341 ttcgcaccca gccacagcgc gtaaagcact tgcgcctgtt gtagcggttc gccgcaaaag
    47401 gttaaacaat ggctattacg accattttcc agcgcctgag agagcagggc gatcacgccg
    47461 cgagcacctt tatccattgc gctgcgcata tcttctgaca gatcgcacac ttcggcagag
    47521 agttttactg tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc
    47581 tggtaataag ccagtatgcg gtcgcggtag ttaccttctc cggattgcag caactccgtc
    47641 agtcgctggt gatatgcggc gtaatggcgc tcaagcatgg caacgccaaa cgcttcttta
    47701 gagcgaaagt agtgatagaa ggaccctttc ggcacttcag cggtttttag taattcgctt
    47761 agccccatcc cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt
    47821 tcacgagtat catgttcggt gtgtttgttc atgtgttgga gtgtagtaga ccagtcggtc
    47881 tagcgcaagc aaagttgcta ctttggacat taacagcttc aataatggta ataatcttat
    47941 ctgaggaggg cgaagtggcg gagcaattag agttctttcc cgtccagagc ccgtgccggg
    48001 gaatttgcca gtctgacgaa cgcgggtttt gtcgcggctg ttttcgaagc cgggatgagc
    48061 gttttaactg gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc
    48121 agcgattaat gcgcaaatta cgtgcaaata agccagcgtc atcagacgaa ccggagcaac
    48181 catcactctt ttgagcctaa tcttgcgtat actcctctca tatctgataa gaggaagcga
    48241 atatggttca gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg
    48301 gctactggcg attgatggac tggaatatgt ccgcccgcca gctggtcagt tttattgaag
    48361 agcatctgga tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt
    48421 gcgaagcggc gtttggcgag gcgctgaaac tggccccaca tctgcgtgaa cggatggaaa
    48481 tcgtcagcaa atgtggcatt gctacgactg cgcgtgaaga aaacgtcatt ggccattaca
    48541 tcactgaccg cgaccatatt attaaaagcg ccgaacagtc gctgattaat ctcgcgaccg
    48601 atcatctgga tctgctgtta atccaccgcc cggacccgtt aatggatgcc gatgaagtgg
    48661 cggacgcgtt caaacatctg catcagagcg gcaaagtgcg tcattttggc gtatccaact
    48721 tcacgcccgc gcaatttgcc ctgttgcaat cacgcctgcc gtttaccctt gccactaatc
    48781 aggtggaaat atccccggta catcagccgt tactgctgga tggcacgctc gaccaactgc
    48841 aacaactgcg tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg
    48901 atgattattt ccagccgcta cgtgatgaac tggctgtggt ggcggaggag ttaaacgcgg
    48961 gctcgattga acaggtggtt tacgcctggg tattacgttt accgtcgcag ccgctgccaa
    49021 ttatcggctc aggtaaaatt gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa
    49081 tgacccgtca acaatggttt cgtatccgta aagcggcact ggggtacgac gtaccgtaag
    49141 cacttttagg aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg
    49201 cacaatgaac ggaggtccta tgaaacgttt tagtctggct attctggcgc tggttgttgc
    49261 aaccggcgca caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt
    49321 tgggcagtca attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc
    49381 cgatctgaaa gcattacccc ccggtgaaca tggcttccat attcatgcca aaggaagctg
    49441 ccagccagcc accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga
    49501 tccacaaaat accggcaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc
    49561 tgcactggta gtcaataatg acggcaaagc taccgatgcc gtcatcgcgc ctcgtctgaa
    49621 atcactggat gaaatcaaag acaaagcgct gatggtccac gttggcggcg ataatatgtc
    49681 cgatcaacct aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta
    49741 attaagaggc cagcgtacct tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca
    49801 gccgccagac aattgccgcc agctcacggg cggcaggttg atgatgacgg gcaaggctgt
    49861 cgcaaatccg ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct
    49921 cactcatcac tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg
    49981 aacgcgattc ccagtcgcgt aattgccaga caacatgaga acagttcagc agcactacac
    50041 cccagcgtaa taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt
    50101 gatgataagt gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagttgat
    50161 cgacaaaatc ccgacgcagc gcgcgaatat ggcgacggct tttacgagca tccgatcctg
    50221 gacgcagaat ggcgaacgct aaccacgcca acgcgacgcc aacgattttc gccagattat
    50281 cgttaagaaa atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca
    50341 taaaaacaat cagttgcccc cacagtgcgg caaattttgg catctgcaat ttaagaagct
    50401 gcatcgtcgc cagcagtgga aagagaaaca gcaaaaattg ccacagatca ctaatctgga
    50461 ccatcaggcc aaatttgacc acaaagctga atagagaaag taacaccagc gtgcgcatca
    50521 gcagtgacaa cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg
    50581 ccagcgttaa tgcattggca ccggcatccc attgcgaagc aatactccat gcgccaatca
    50641 tcatcaacgt acaaaatgta cgcagcccgc tccacatagc ttcggcgtta tcggtatgac
    50701 gagccaggcc ggatgtccgt gggagtctgg tatgatcatc tacaccgctt tgcagacgat
    50761 gtaattcctg actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg
    50821 cgacgtgccg atagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg
    50881 tgtaaacatc tgtttgcgaa ctggcgagcg ccgtcagcaa ctgttcgaga atttctcgtg
    50941 tggcacctgg cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg
    51001 tcatacgtaa ttgctggtgg agcagcgcat taaggcgcgc gttttgctgg cgaaaacgat
    51061 agtggctcca gaaagcctgg atacgcagca aattcatggt cagtatctgc ccaatcaccc
    51121 cttcatgtgc tgcacgaatg gcatcggttg tttcaggctg ccagagtaaa ctggcatgtt
    51181 ccagtaatcg ggcgtgcatg tttttcaatg cggttaaaag ggcagtagca tcggaactgc
    51241 tcggcaggat catcatcatc atgccgccgc acaaaatacc gacaatcacc tcgcaaacgc
    51301 gcgcctgagc gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga
    51361 tgatggcagc cgtgtagccc gccagttgaa atgcatacgc gacgttattc gtgaagtgcg
    51421 cacaggccca ggtacaaaag ccaagccacg ccgacatgct caatagaaaa aaccacggct
    51481 cattgagcgt atgtccggca agaagcagtg ccgcaatggc tcccagtaaa ctgccagcga
    51541 tgcgtccgag gcttttgctg atgacaccgc caacggtggg aaagctaacc actgcagccg
    51601 aggtcatcgc ccaatagggt tcatccagat tcaaataata ggcaaccgtc agcgccagac
    51661 acatagcaat ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ctgaaccagg
    51721 gcaaattgcg caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt
    51781 ggtgcccgac accagcgtga catcgcctgg aactttatcc agcgcaaagc ggacgggaac
    51841 gcgttgggcg agacgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc
    51901 actttcaacg ctctgatcat aaatcgcccg accgatactg aaaacgtgac cctgtaacgt
    51961 tttgttatca ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt
    52021 ggtttcttca aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg
    52081 tttcccggtg tcagcgtaat cgccgatgcg ggtagtgagg ttagtcaccc atccactcac
    52141 cggggcgcga atttccgttt gcgccagttg ccattgtgtc tgtttcagcg tcgcctgtgc
    52201 ggcatcaaca ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc
    52261 agaaataaaa ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag
    52321 gtcagattgc gctttcgcca gctgtgcctg tgcgttcagc tcggcgatct gaaagggcgt
    52381 tttatcgatc gtgagcagaa gatcccccgc gttaaccagc tggttgtctt ttatattcag
    52441 ctcaacaata cgtcccgaca cctgtggggt gatagaaacc tgttctgcgc gtattttccc
    52501 gtcgcgtgtc cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc
    52561 aactaccgct acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac
    52621 gcaatcagta ttgccagagc aaggcaaacg caaatcgcaa acagcgataa atccattaac
    52681 aagggatgcc agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc
    52741 cagatgacga atccaaacgc gaatgctttg aaaaacggag gaaagtagac ggacgcaccg
    52801 aacaccagat cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt
    52861 cctttacgac cgaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta
    52921 cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag
    52981 caatacaatt gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa
    53041 ggagatgaaa ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg
    53101 tgctctgata gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt
    53161 acacaatatc catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg
    53221 catcgagcag ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc
    53281 gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga
    53341 gccgctgatc agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa tcttacatgg
    53401 catctccgca gaggaactgg aacaactgat tacgctcatc gcaaaacttg agcataatat
    53461 cattgagtta caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca
    53521 cgcaaactta tttagcgcgg agaaacggtc acctgactgc cattgctggc cagtacgaca
    53581 cgttggcccg gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca
    53641 tcgtctttac gaatttccag ctcgacacct tgcgttttgt tcattgcact ctgtacgccc
    53701 tgaccagcta cgccacctgc aacagcgcct gctgcagtag ccagagaacg cccggttccg
    53761 ccaccaacag tatttcccag gaaaccacca agaacagcac cgccaattgc accgataacg
    53821 ttggaatcat caccgccctg aatctgtacc ggacgtacgt taacgatggt tccatagctg
    53881 acattctgta cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta
    53941 ttaacacaac cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt
    54001 gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg
    54061 gcatatgtga atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag
    54121 gatcaaccaa ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac
    54181 taccaacggt aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg
    54241 tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta
    54301 tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg
    54361 tcgcacagct ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg
    54421 atatttgcca gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg
    54481 ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag
    54541 atatagttgc gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac
    54601 cacacacttt acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg
    54661 tcggtgattt tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg
    54721 cgttccatca tgccttgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg
    54781 gcatcgccaa tctgtcactg ctcattcctg ggcagccggt tggcggctac gataccggtc
    54841 ctggtaacat gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag
    54901 atgccgagtg ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg
    54961 acccatattt ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactacggct
    55021 ggctggagcg ccatttacgc cattttccgg gtgttgatcc ccgtgatgtt caggccacac
    55081 tggcagaact caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac
    55141 gattgatggt atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat
    55201 tactgccagg cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg
    55261 aagcattggc tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc
    55321 cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttcccg gctaacccgt
    55381 gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg
    55441 agggcgtatt gccctccaga ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta
    55501 ctgatctgtt tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcgc
    55561 atgcaaaccg atacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat
    55621 aatccgcgcc aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc
    55681 atttcagcct ctggtgcacg ttacactgac ggaatctatg ttttctggtc gaaaggcgag
    55741 gaagcgactg tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaattca
    55801 cggcgttgag atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa
    55861 gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac
    55921 caaaggcggg ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg
    55981 gctctctcag gcttgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt
    56041 ggatgaacat ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg
    56101 catggtgttt tacaccaacc tcggtagccg taaagcacat caaattgaaa ataatccgcg
    56161 cgttagcctg ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc
    56221 agaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca
    56281 gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag
    56341 taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagcttttg
    56401 gggcggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct
    56461 gcatgaccgc tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc
    56521 ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc
    56581 tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac
    56641 atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta
    56701 gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc
    56761 tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta
    56821 tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg
    56881 ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact
    56941 gttcaggagt gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt
    57001 ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg
    57061 accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg
    57121 gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac
    57181 ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt
    57241 ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat
    57301 cagaatcagg tgtttggcct gaccgttccg ctgatcacca aagcagatgg caccaaattt
    57361 ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc
    57421 taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc
    57481 tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca
    57541 ccgcgcgccc agtatgtact ggcggagcag gtgacccgtc tggttcacgg tgaagaaggt
    57601 ttgcaggcgg caaaacgtat taccgaatgc ctgttcagcg gttccttgag tgcgctgagt
    57661 gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc
    57721 gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt
    57781 aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac
    57841 ttctttaaag aagaagatcg tctgtttggt cgttttacct tattgcgtcg cggcaaaaag
    57901 aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac
    57961 tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt
    58021 tgtttatggt catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa
    58081 cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg
    58141 ctgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa
    58201 attacacacc tgtgatgccg tattaagtgg ctatctggga tcggcggagc agggtgaaca
    58261 tatcctcggt atcgtccgtc aggtgaaagc cgcgaatccg caggcgaaat atttttgcga
    58321 tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca
    58381 tgtgcggcac ggtttgcctg ccagcgatat cattgcgccg aatcttgttg agctggaaat
    58441 actctgtgag catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat
    58501 tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga
    58561 ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg catatcagcc gtccactggt
    58621 ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt
    58681 gaaactgctt cagggggcaa cgctgcagga ggccctggaa catgtgaccg ctgcagtata
    58741 cgaaatcatg gtgaccacca aagcaatgca ggaatatgag ttgcaagtgg ttgctgctca
    58801 ggatcgtatt gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt
    58861 tcggcccaca acgaactgtg ggccgacggc tattacttta agccttccgc tgacagcgcg
    58921 tcttgtactt ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct
    58981 tccagattca gtttcaccgc gtatgcccag cgcagtaccg taaacagata ggcatcagca
    59041 attgtaaatc tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc
    59101 agcttcttct ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga
    59161 aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt
    59221 ttatagcggg aaatactgtt taccgatgcc agcaactggc ggtcggggac gctgtcggca
    59281 agatactgca taatcgctac accttccgtc agcaaagtac cgtcatccag cagcaatgca
    59341 ggtacctgtc ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc
    59401 attaaatcca cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa
    59461 gcgagagagc aggcgcccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg
    59521 tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc
    59581 ttgtaaaaga gtttccctga attttacgct acagcagctt tcgccgcttt gtcagcagcg
    59641 tcatcctgcg tcatgcggtg cagtttcggc gcggtaaaca gcatcagtac tgcaataacg
    59701 gcagtagcga caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga
    59761 tcggtaacgt tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca
    59821 ccggcagtgg tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt
    59881 tgagcaacca ttgccagacc cagaccagag atcataagtt ccccgatact ctgcaggcca
    59941 tagcttgcga ccagccagct tacagacacg atacctgcgt cagacgcgaa ttttgcaccc
    60001 agcggcagaa tcaggaacgc accagaacac atcaccatac cgattgcaaa cttagttggc
    60061 atcggcaggg tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg
    60121 atccagaacg ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggtg
    60181 tgttcaacgt tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg
    60241 aagaagataa tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca
    60301 cctttcatcg cgaaggcttc tttaccgaag ataaccacga taccaaaggc aacaacgccc
    60361 agcgccatac gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc
    60421 acaacaccaa taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt
    60481 gaaccgtact gtttaaccca gcgttggcag aaggcgaagt taacaatagt gatcagcagg
    60541 cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggggta
    60601 gcgatcatag agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg
    60661 tccagacgcg ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta
    60721 aacaggccgt taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga
    60781 ccagaccagg caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg
    60841 cgtttagtac ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc
    60901 agggcactaa aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta
    60961 accaggtaaa cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca
    61021 atcgagaaga tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca
    61081 gttggttttt ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg
    61141 ggtgctttct cgcgttatgc ctaaaaacat acgcgcaatt gttatagtgg gaggggaaac
    61201 ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat
    61261 ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa
    61321 aaaacgtaac ggcagatttt ttcactgcat tgagaggggg atgatggctt gacggcgata
    61381 tgttctgcta tttagcgttt ttcctagtga gatagtccag tttctgaaaa atagccagtg
    61441 taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc
    61501 ttgcaatgtt tttacattgc cacagcatcg tgatcttgat cacgaatgaa tagaaaattt
    61561 cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga
    61621 taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag
    61681 gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat
    61741 tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat gcaggtataa
    61801 cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc
    61861 agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa
    61921 acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt gtttaatacg
    61981 acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg atcttccgga
    62041 acctcgccat tatgctgctc cagtaagata cggcaggttt tgatgatatt ttctgctttg
    62101 ctgttataaa gcccaatcgt tttgatatag gttttcaccc cttcaacgcc cagttcaagc
    62161 atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt aacactgaca
    62221 tcggttgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta
    62281 agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc caggcgtttt
    62341 gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt
    62401 cagtacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc atcagtccca
    62461 ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa
    62521 tctccacgcg taatactttt gcccagctac ctaacagcga atctgcaccg tcaaacaacg
    62581 tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgccca
    62641 taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt
    62701 cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt
    62761 gatacaggcc aaaagcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga
    62821 tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa
    62881 tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag
    62941 tggcagtgga cgtgaccgcc aacagaggac aaaggccgag caactggacc agcgcagagt
    63001 tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt tattctccac
    63061 aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg gcgcgtttta
    63121 ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac
    63181 catctttctt caccgcccag tgcgcatcat ccgcaccact gattttttta cccgcaaaat
    63241 gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc gtttcgtggt
    63301 gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa
    63361 tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc ggtttgtcat
    63421 cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gttaccagat
    63481 agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga tcaaataacg
    63541 ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg
    63601 cagttaaccc tgttgaaccc gctgcaaata gcgccaacgt aatgccgtgt tttcggatag
    63661 ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgggtg tagtaatcga
    63721 tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc
    63781 cgaaactgcg gatcaaccag actaataagc ccgcaagcgc gccgaaaata agacgaccac
    63841 gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg agcatggtcg
    63901 ctccagacag cagatgaatt tgcggtgcgg ccagtgtttc tggtgagaac aaccagccca
    63961 acgttgcgca taacgtcagc gttactaaga agctgagggg aatatgccag cgaatagctt
    64021 tctgccatag caaccacacg ccgccagcca gccaggcgag atttacccat tgccaaccag
    64081 cgcccgccag cataccgctg tagatcggat attgcataat ctgttcaacc gaatgaccgg
    64141 cacggacaga ggttttaaat gtatccagcg gtgtcgcctg actaatgcca tcaataccta
    64201 agcgtagtgt gttcatatca ccaccactgg cggtatgccc gctgaaaata acctgtatgg
    64261 cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct
    64321 gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt
    64381 gccccagacc gccatacaac tgtttagcga tgataaccgc aaacaccgta cccagcacaa
    64441 ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct gtcagcaatg
    64501 ctgagttatc tttcaacgtg gcggctaccg actgcttgcg tagtttgagt acgagagctt
    64561 cggctaacag agcactaacc gatgccaaca ggatctgaac gagagtaccc caaccaaaaa
    64621 accacagttg cgctgcgatt cctggcacgg ctgcgagcaa cactaacagc ataatgcgcg
    64681 atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct
    64741 cgtttacaac cttttgctgg gcggcttttt tggcctgaac gcgggcaata gccgcggcaa
    64801 ctgccgcttt gcgcggatca acctgttctt ctggtaccgc attagcctgt tgctgttcca
    64861 gcttgcgtgc tttggcgcgt gcaatagccg cttcgacggc agctttgcgc ggatcaacct
    64921 gttcttctgg ctccgcatta gccggttgct gttccagctt gcgtgctttg gcgcgggcaa
    64981 tagcggcttc gacggcggct ttgcgcggat cgacctgttc ttctggttcc gcattagcct
    65041 gttgctgttc cagcttgcgt gctttggcgc gggcaatagc ggcttcgacg gcagctttgc
    65101 gcggatcgac ctgttcttct ggctccgcat tagcctgttg ctgttccagt ttgcgcgctt
    65161 tggcacgggc aatagcggct tcgacggcgg ctttgcgcgg atcgaccggt tcttctggtt
    65221 ccgcattagc ctgttgctgt tccagcttgc gtgctttggc acgggcaata gcggcttcga
    65281 cggcagcttt gcgcggatcg acctgttgtt ctggttccgc atttgcctgt tgctgttcca
    65341 gcttgcgcgc tttggcgcgg gagatagctg cttcgacggc agttttacgt ggatcagtaa
    65401 cggttgctgc gtcgttagtt tgctgcagtt ctgcctgttt cgctctggct tgcgctttac
    65461 gggcttcccg tgctgcaata attgcactgt tatccgggcg ttcgcccgct ttaatcacaa
    65521 taggctgtgt agcctgggcc tgtttctctt tcacccgcgc cagagcggca gcaatcgcat
    65581 ctttatcttt ggctgcaggt tgaacggctg cgctcttatg tcgttcaagg cgagccgctt
    65641 tttcgcgctc cagacgagcc tggcgcgctt cgaaacgcgc tttggcttct gcggcgcgct
    65701 tttcttcctg acgaatagcc gcaatttcag ctttttcctg acggaaatat tgcaccaggg
    65761 gaatattgct cgggcaaacc cacgcgcaag caccacattc aatgcagtcg gcaatgttat
    65821 gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca gtacaactgt tgcggcaaaa
    65881 gatccgcagg gcaggcgtca gcacaggcgc tacaccggat gcagctttgt tcttcctgtg
    65941 gttcgccaag ctcattggca gagggagcca acagacagtt ggtaatcttt acgaccggga
    66001 catccagcca tggcaaggta aagcccatta gcgggccgcc cataatcacc atttgatcgg
    66061 cagaggggca gaatccggcg tcattcaata aatgacgcac tggcgtcccc agccgtgccc
    66121 agacgttgcc cgggcgagcg attgcttcgc cagtcagggt tacgacacgc tcggtaatcg
    66181 gctcgccatc aataacggca cgtttcactg cataagcagt gccgacgttt tgcattaata
    66241 cgccgatatc agatgaacgc ccgccatgtg gaacctgctt cccggtcaga atgtaggtta
    66301 attgtttagc accgccagaa ggatatttgg ttggaatcac ccgcagagaa atatcgtgag
    66361 agtccgccag caccgcgcgc agcatggaaa tcgcctgcgg tttgttatct tcaatgccga
    66421 taagaatttc gcgtggctgc agaatatgcg caagaatgcg aataccctct acgacctgag
    66481 ccgcgcaatc ctgcatcaaa cggtcatcgg cggtaatgta cggctcgcac tcagcagcgt
    66541 tgataatcaa cgtttcaatc ttatctccgc cgccctgcaa tttaacgcct gtcgggaatc
    66601 ccgcaccacc cagcccggca acaccaaact gatgaatgcg ctcgattaac tcttcgcgac
    66661 tgcgagagcg ataatcggcc cagccgtctc gtgggatcca gcagtcttca ccatcggcat
    66721 caataatcac gcttaattca gctaaagctg aaggatgagc cgtagagtgg ggcgcaatag
    66781 ccgtaacggt acccgaggtg ggcgcgtgaa caggcagcat tttgccgcga ccacgggtaa
    66841 gcggctggcc gcgcaatact ttatcgccga cgctaacgca caactcacct tcagcgccaa
    66901 tatgctgttt cagtggaatg acaaaacgct gcgccagggg tacctggcgc aggggtgtac
    66961 cgttggactg ggttttcatc tccggtggat ggatgccgcc gttgaaatcc cagattttat
    67021 tttttctgaa tgcagagaat aacttaagca tggtgttcca cgggaatgat acgcacggga
    67081 atggtgttca gatcccattt ccaggagtca ggtgtttctg cgaccggttg caacgagatg
    67141 cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg tacagagatc actcattacc
    67201 gtatgcatgg cacgggtagc gccaacgatg gcgtctaccg gacacgcctg aatacattta
    67261 gtgcagccaa tacagttatt ttcatcaata accgccacca tccgcgcagg cgttagctct
    67321 tgcgcttcgc catccagcgg ctgcggctcg acattaagca actcggcaat ttttagcatc
    67381 acagcttcgc cacctggggc gcaacggttg attttttcac cgttacagct gatggcttcc
    67441 gcgtaggggc gacagccggg ataaccgcac tgaccacact ggctctgcgg taagatttcg
    67501 tcaattttct caacgaccgg atcgtcttcc accgcaaaac ggcgggaggc ataacccaga
    67561 atggcgccaa acgccaggcc cagcaggctc acggcggcaa cggcaatcca gatagcattc
    67621 attacaactt caccaaacca ctaaagccca taaaggccag agacataaga cctgcggtaa
    67681 ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc agccacagca aggcgttcgc
    67741 ggatggctgc gaagagaacc atcaccagcg aaaaaccgac agcggcggaa aaaccgtaca
    67801 gcgccgactg caagaaattg tgcccgagat tgatattcag caacgccacg ccgagcacgg
    67861 cacagttggt ggtgataagc ggcaaaaaaa tccccagcaa acggtaaagc accgggctgg
    67921 ttttgcgcac caccatctcg gtgaactgca cgaccacagc aatcaccaga ataaatgcca
    67981 gggtgcgcag gtaaatcaga ttaagtggga tcaaaatcca cgtatcgata agccaggcgc
    68041 aaatagacgc cagcgtcatc acaaacgttg ttgccagccc catgcccatc gcggtttcca
    68101 gctttttgga aactcccata aacggacaga gaccgagaaa cttgaccagt acaaagttat
    68161 tgaccagtac agttccaaca aacagtaaca ggtagtcagt catttttcgg cctgaaataa
    68221 aaaagccgcc tattatccgt taatccagag caggcgacaa caggttaact gtaaggttat
    68281 tacggattca caaacgtcgc tttcacccgc gacgagcgtt tgaaataggg gacgatcaat
    68341 gcggttgcca acaaagtaaa cagcaactgg cgaacggcta tgccatcttc cacgggtgaa
    68401 aaagcgaacg cttttactgc cagcaatacg gaaataagca gccagataat atagtgttta
    68461 ggaacacaac gccgacgttt aaagaaagcg atggtcaacc acagtgtgta ataccacatg
    68521 gcgatagcag taataaaaga aacgccccat aagatttgtg tcgtcagtgc ttgtgcgccg
    68581 agcgtttgaa atgtttgcgg agaagataac gcagcggtat acagcaacaa cgccagcgtc
    68641 gtacttaaca gagcgactaa aagccaggcc aacgggccaa gcaaccagcc tccaatacgt
    68701 tgtggcgttg tggtggtcat aggttctcca gaatagcgac gcaaacaggc aaatctaatt
    68761 ttaggggcgt tattataagg cttttataac cgatcgctac tttctttatt ggacatagtg
    68821 ccagacggac ttcggtacct ggccgaggtc aaacaaacgc ccgccagaaa ccagctctgc
    68881 gcgacggtga tcggcagcac gatacatatt gatcagttcc tgatcgtccg tcagggtgta
    68941 attaagatgg tcgtaaagtt tttccagact ttcgagtgaa ctgattttgc gaaattttaa
    69001 taagtagtcc tgaacagtca taaaaggtcc ataaaataat taataccaaa ggggtaggta
    69061 ttattagtga tgataaaatt tcaccaaaag tgaacgcttg tgttattaca ataataagaa
    69121 tgcgctgatg gcaaatattc agggatgaaa aactgctatg aaaagagtaa cgcgctttac
    69181 tatccctgac aatcaccaac aacaccgaac aagataataa attcctggtt taatatccga
    69241 caagtgaaaa catgcaccct gacgggcagc atgtcgctct acaagcgcag agcttacttg
    69301 tgttgtaccg tagtactctg ttcaggtggc tgatagttgt caatgtgact cgccacgcca
    69361 agaagaatga ctgaaacgac aagaacgatc caacctgtta attcaataag acgattcatt
    69421 acaactcaca tctctgttga ttgatccatt aacttcaggg ggtaaatgtt acttagcaat
    69481 aatagctcca gcaagatttt tactgaggtt tttgcgatat ttagcttttg tcgttggaaa
    69541 aatcgctatt tttttgactt aagttaaaca acatccctta ttgctggcag gttattaaac
    69601 tgttgagcga gggtaaagga tagtgtcaaa tagccatcat acttcaatga gaggcaatga
    69661 catgagcgac aacatccgtg ttgggttgat tgggtatggt tatgcgagca aaaccttcca
    69721 tgcgcccctg attgcgggca cgcccgggct ggaactggcg gtaatctcca gcagtgatga
    69781 aacaaaagta aaagccgact ggccaacggt tacggttgtc tctgagccga agcatctgtt
    69841 taacgatccc aacatagacc tgattgtcat tcctacaccc aacgataccc atttcccgtt
    69901 agccaaagca gcgcttgagg cgggtaaaca tgtggtcgtt gataaaccct ttaccgtgac
    69961 actgtcacaa gcgcgagagc tggatgcgct ggcaaaaagc ctggggcgtg tgctgtctgt
    70021 attccataac cgtcgctggg atagcgattt cttgacgcta aaaggtttac tcgcggaagg
    70081 cgtgctgggt gaagtcgctt actttgagtc tcattttgac cgcttccgtc cgcaggtgcg
    70141 cgatcgttgg cgtgaacagg gtggcccagg cagtggtatc tggtacgatt tagcaccgca
    70201 tcttcttgat caggctatta cattatttgg tttaccggtc agcatgacgg tggacttggc
    70261 acagttacgg cctggagcgc agtcgaccga ttatttccac gccatcttgt cctatccaca
    70321 gcggcgagtc attttacacg gtaccatgct ggcagccgct gagtcagcac ggtatatcgt
    70381 gcatggatcc cgaggcagtt atgtgaaata tggcctcgat ccacaggaag aacgtctgaa
    70441 aagtggcgag cgtctgccgc aggaagactg gggctacgat atgcgtgatg gcgtacttac
    70501 ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg acggtgcctg ggaattatcc
    70561 ggcttactat gcggctattc gtgatgcgtt aaatggcgat ggtgaaaatc cggttccggc
    70621 aagccaggca atccaggtaa tggagttgat tgagctgggc atcgaatccg ccaaacatcg
    70681 cgcgactttg tgccttgcgt gaaaatggat ggtgcaattt gcaccatcca tttttgttac
    70741 ttcgctgcga ctttttctcg cagtgcgcgt ttttcctcag cgctgaggaa agccatttcc
    70801 agaccattaa tctgtgcctg gcggatttgc tcgcgggata acccagcagc tggcgcggca
    70861 acgttatatt cgtgaatgat atccactccc tgtacgccgg gatcgtcagt gttaatgctg
    70921 gcacgaatgc catgatcaag gaacgttttc agcggatgtg cagccagctc agccacggtg
    70981 ctggtctgaa tattggaggt cagacaggat tcaataccaa tttgttgctc ggcgagaaaa
    71041 tccatcagcg cccgatcttc aatggctttt acgccatgtc caatacgctc cgcacccagt
    71101 tcacgaatcg cctgccagat gctttccggc ccggcagctt cgcctgcatg gacggtaata
    71161 tgccagcccg catcacgcgc gcggttgaag tgagaaagaa acagacttcc cgggaaacca
    71221 agttcatcac cggctaaatc aagtgcggta atctggtcac ggtgggctaa aaaggcctcc
    71281 agctcttgct gacaggcggc ttcgccgaag gttcggctca taatgccgat aagcttcgcc
    71341 tgcacaccaa aggtgcggca accttcacgt acgccatcga tcaccgcttc aacaacacct
    71401 gcaacaggca gctgatgtgc cattgccatg tagcctggag aaaaacgcag ctcgacatag
    71461 tgcaggccgt gacgggctgc atcttcaatg ttttcaaatg ccacgcggcg acaagcatca
    71521 agagaggcga gaactttaac gccccagtca agtttggtca gaaagctcac cagatcgggt
    71581 tcgttggcaa tgacctgaac gtggggaatc agtgtttcca gggattgtgc aggaagcgag
    71641 atattatact ggcggccaag ttcaagaatg gtctggggac gaatgttgcc atcaaggtgg
    71701 cgatgaatat cagttaatgg cagggtggta tcaatcatgg tcgcactctt tttctggata
    71761 taaaagtgcg caccattata aaaacaaagc agggtaaaaa tccacgattg cgcaacgatt
    71821 ttttacgttg cgcaattggg gttagcgaac agcgcggatg gcgttaatta atccagccac
    71881 acctttttcc agtttcgaac gtgggcagcc ggcattgagt cggacaaaac cacgaccttc
    71941 ttcaccgtag gtataccccg gcatgatcgc gactttttct tgttcgataa gtgctttttg
    72001 caaggcgttg tcgtcaatat tcaacggacg taaatcaagc caggccagat aagtggattg
    72061 cgggatctgc cagttgagtt caggaaacgt ggcgttcatt ttatctgcga tatacgtcag
    72121 gttatctttc agatagacgc gtaaggcatc cagccacggt gcaccttgct gataggcggc
    72181 gatatgggca gttaacgcca gtaccgaagg ggaagaaagc ccatcacgtc ctttcagtgc
    72241 cgataaatag gcatcacggc tgctgctatt ttctataatc ccgtaagcac cggtcagcgc
    72301 gggaatattg aaacttttcg atcccgacgt cagcaacgcc cagtctcccc gcgccacatt
    72361 actccaggga atatgcggct gctcgcccca aaccatatcc atatggattt catcggaaat
    72421 aaccctcaca ccatgacgct cgcacaggtc agccatgatc tccagctcat cgcacgtcca
    72481 cacttttccg gtaggattct gtgggctaca caggagcata attttacatt ctggtttcgc
    72541 caacacggct tccagcttgc ccatatcgca aaaccagccg tcagcctgct tctctaaagc
    72601 aacgggcatt actgtgcgct gattaccttc aatggccttg taaaatgcgt cataggcggg
    72661 tgtgtggatc accacgcctt cacctgtttc agaccactga cgaatcagtt ctgagaccat
    72721 atagataaca gaagggccat acaccaccga ctgaggatcg atggcggtgt aatgctgggt
    72781 ggaaaaccag tgggcaatag ccgcgagaaa ctcatcgttc ttccagcggc tgtagccaaa
    72841 cacgccgtgc atcaggcgct gattcagcgc ctcgataatg cagggggcag tggcaaaatc
    72901 catgtctgaa atcgtgaaag gtaacaggtc agcagtgccg aaacggtcag cgacataatc
    72961 ccactgcgta caccatgtgc catgacgatc cacgaccttt gaaaaatcga acatatctta
    73021 tccttatgcc tggacagtat gcatcagacc ggccatttca tcttttactg actgaacttg
    73081 tggcccgata acaacctgca ggttatgttg attaagttgt actacgccaa ttgcccgatt
    73141 gtccttcagt gcctgcacat taacaagcga catatctttc acagacaaac gcaaacgggt
    73201 aatgcagtta tcaaggctga caatattgtc ggcaccgcct aaggcttcga ggattgccgg
    73261 aacgttgtaa cctgatttac ccggcgcacc ggcaacggct ttttcgattg aactggccac
    73321 ttcgctatcg cgccccgggg ttttcagatt gaagcgggtg atagcgaaac ggaagatgac
    73381 gtagtaaacg acaaaccaga ttgccgccac cactggcacc atgtaccact tggttgacag
    73441 accatgcaaa ataccgaaca ccacgaagtc gatgatattg ccgtcggtat taccgatggt
    73501 gacgccgagc acagacatga cggtgaagcc gaggccggtt aacagcgcgt ggatgacata
    73561 cagaactggc gctacgaaca ggaacaggaa ttccagcggt tcggtcgtgc cgccaacgac
    73621 acaggcgatc aggccagaaa tcagcagccc tttaatttta tggcgatttt ccgggcgcgc
    73681 gcagtgatac atagctaacg ctgcacctgg cagaccgccg agaaacgcag gcattttgcc
    73741 ttgcgaaagg aaacgcgtgg cgctttcaga aaaaccgtga gtggtcgggc aactcaattg
    73801 cgcctggaag atggtcagtg caccgctgac ggtttgaccg cagacttcct gcgtgccgcc
    73861 tgcgtcggta aagcgaatta atgccaccag aatgtgatgc agaccaaacg gcaacagcag
    73921 acgttcaccg gtaccaaaca gcatcggtcc gaaatcaccc gcgctattga tcatatggcc
    73981 taagccgcta atacccatgg cgaaaattgg ccagactaat ggaatcacca gaccgacaat
    74041 gcccatcacc agcgaggaga tgattggtac gaagcgcgta ccgccgaaga atgccagcgc
    74101 atccggcagg cggatattat ggaatcgctc atgcagcatc cagacgataa taccggcgat
    74161 caccgcaccg aggatcccgg tgtcgatcga ctggatccca agaatgctct ggatgttatt
    74221 ggctttcaga acggcggcat ccgtggttgg cagaatgcct ttattggtca accagaagtt
    74281 taccgcgagg ttcattaccg cataaccgac gaagccagcg aatgccgcta cgcctttgtt
    74341 ttcgcgtgcc aggcccagcg ggatggcaat acagaacatg actggcagga aactaaaagc
    74401 aaacgagcca atcttactca tccaggtaaa gatagcctgc aacacgggat tgcccaagac
    74461 cgggatcagg gtgatgacat cgtggctact aagggaacta ccaatgccga gcataatgcc
    74521 gcagaacgac aataatgcca cgggtaacat gaaggttttg cctaactgct ggaagaactc
    74581 ccacagcgtg actttcggtg ctgttttcgc cgtcataaaa cgactcctcg tagagaacaa
    74641 tctggattca gtaaattgcg cgagaagata aaacgtttta tcaaatttta gtgaggcata
    74701 aatcacatta cgcaacgata atagcgggta taagataaat aaaaggtaaa acgttttatc
    74761 tgtcacaaaa tcagggagta ggtcatctgc atggctaccg ccaaaaaaat aaccattcat
    74821 gatgttgcgc tggctgcggg cgtgtcggta agtaccgttt cgctggtgct tagtggcaaa
    74881 gggcgaatct ctaccgccac aggagaacgc gttaacgccg ccattgaaga gctgggattt
    74941 gtgcgcaatc gccaggcgtc ggcgctgcgc ggcgggcaaa gcggcgtcat tggtttgatc
    75001 gtccgtgatt tatctgcgcc gttttacgcc gaattgacgg ccggattgac ggaagctctg
    75061 gaagcgcagg gacggatggt ttttttgctt cacggcggta aagacggcga gcagctggca
    75121 cagcggtttt cactgttact gaatcagggt gtcgatggtg tggtaattgc cggggctgca
    75181 ggaagcagcg atgacctgcg acggatggca gaagaaaaag ctatcccggt gattttcgct
    75241 tcccgtgcca gttatcttga tgatgttgat acggttcgcc cggacaacat gcaggctgca
    75301 cagttgttga cggagcatct cattcgcaat gggcatcagc ggatcgcctg gctgggaggg
    75361 caaagttcct cattaacccg tgcagaacgg gtggggggct attgtgcaac tctactaaaa
    75421 tttggcctgc cgtttcacag cgattgggtg ttggagtgca cttccagcca gaagcaagcc
    75481 gcggaagcta tcacggcgct tttacgtcat aacccgacca tcagtgccgt ggtttgctat
    75541 aacgaaacta ttgcgatggg ggcatggttt ggtttgctga aagcagggcg gcaaagcggg
    75601 gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc tggcggcatt taccgatgcg
    75661 acaccaacca cacttgatga tatacccgtt acctgggcca gcacgcctgc gcgggaactt
    75721 ggtaccacac ttgcggatcg catgatgcaa aaaatcaccc atgaagagac gcattcacgc
    75781 aatcttatta ttcccgcccg gctcattgcg gcgaaataat cctctcttta tctgctatac
    75841 ctggtagtgt cctttcctca aggttaatga taaaaaatgt ggcacacagg cggtgccttt
    75901 tatttttact gaagttcaca ggaggtttat gtgtttaatt ctgacaacct gagactcgac
    75961 ggaaaatgcg ccatcatcac aggtgcgggt gcaggtattg gtaaagaaat cgccattaca
    76021 ttcgcgacag ctggcgcatc tgtggtggtc agtgatatta acgccgacgc agctaaccat
    76081 gttgtagacg aaattcaaca actgggtggt caggcatttg cctgccgttg tgatattact
    76141 tccgaacagg aactctctgc actggcagac tttgctatca gtaagctggg taaagttgat
    76201 attctggtta acaacgccgg tggcggtgga cctaaaccgt ttgatatgcc aatggcggat
    76261 tttcgccgtg cttatgaact gaatgtgttt tcttttttcc atctgtcaca acttgttgcg
    76321 ccagaaatgg aaaaaaatgg cggtggcgtt attcttacca tcacttctat ggcggcagaa
    76381 aataaaaata taaacatgac ttcctatgca tcatctaaag ctgcggccag tcatctggtc
    76441 agaaatatgg catttgacct gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg
    76501 gcaatattaa ccgatgccct gaaatccgtt attacaccag aaattgaaca aaaaatgtta
    76561 cagcacacgc cgatcagacg tctgggccaa ccgcaagata ttgctaacgc agcgctgttc
    76621 ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg
    76681 gtacaggagc tcaattaata cactaacgaa ccggtaaaca gccgtatgtg ttgtttaccg
    76741 ggatagcgtg tgcaattcat caacgtctct gctaaacaac tggcagccaa atcacggcta
    76801 ttggttaacc aatagctgga tgaaaagcat acgaatagag tgtgccttcg cactattcaa
    76861 cagcaatgat aggcgctcac ctgacaacgc ggtaaactag ttattcacgc taactataat
    76921 ggtttaatga tggataacat gcagactgaa gcacaaccga cacggacccg gatcctcaat
    76981 gctgccagag agattttttc agaaaatgga tttcacagtg cctcgatgaa agccatctgt
    77041 aaatcctgcg ccattagtcc cgggacgctc tatcaccatt tcatctccaa agaagccttg
    77101 attcaggcga ttatcttaca ggaccaggag agggcgctgg cccgtttccg ggaaccgatt
    77161 gaagggattc attttgttga ctatatggtc gagtctattg tctctctcac ccatgaagcc
    77221 tttgggcaac gggcgctggt ggtcgaaatt atggcggaag ggatgcgtaa cccacaggtc
    77281 gccgccatgc ttaaaaataa gcatatgacg atcactgaat ttgttgctca gcggatgcgt
    77341 gatgcccagc aaaaaggcga gataagccca gacatcaaca cggcaatgac ttcacgttta
    77401 ctgctggatc tgacctacgg tgtactggcc gatatcgaag cggaagacct ggcgcgtgaa
    77461 gcgtcgtttg ctcagggatt acgcgcgatg attggcggta tcttaaccgc atcctgattc
    77521 tctctctctt tccgcgggct ggtgacaact gagcccgcgt ttcatacttt aatttccctg
    77581 tacaaaatac aatctcgccg gagcggggaa ttcctcccta aaatattcac tgtagccata
    77641 tgtcatgaga gtttatcgtt cccaatatgc tcgaacgaac gttcgattgc ttattttatg
    77701 gcttctgtca acgccgtttt aaagattaat gcgatctata tcacgctgtg ggtattgcag
    77761 tttttggttt tttgatcgcg gtgtcagttc tttttatttc catttctctt ccatgggttt
    77821 ctcacagata actgtgtgca acacagaatt ggttaactaa tcggattaaa ggttgaccag
    77881 tatttttatc ttaatgagga gtcccttatg ttacgtcctg tagaaacccc aacccgtgaa
    77941 atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgat
    78001 cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt
    78061 aacgatcagt tcgccgatgc agatattcgt aattatgtgg gcaacgtctg gtatcagcgc
    78121 gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc
    78181 actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca gggcggctat
    78241 acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcacc
    78301 gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac
    78361 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactacgc cgggatccat
    78421 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
    78481 catgtcgcgc aagactgtaa ctacgcgtct gttgactggc aggtggtggc caatggtgat
    78541 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggcaccagc
    78601 gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa
    78661 ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgctgcg cgtcggcatc
    78721 cggtcagtgg cagtgaaggg cgaacagttc ctgatcaacc acaaaccgtt ctactttact
    78781 ggctttggcc gtcatgaaga tgcggatttg cgcggcaaag gattcgataa cgtgctgatg
    78841 gtgcacgatc acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac
    78901 ccttatgccg aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa
    78961 actgcagctg ttggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg
    79021 aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaggcgca cttacaggcg
    79081 attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc
    79141 aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca
    79201 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
    79261 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
    79321 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
    79381 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
    79441 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
    79501 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
    79561 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa gggcatcttc
    79621 acccgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
    79681 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca
    79741 ccatcgttgg ctacagcctc ggtgacgtcg ccaataactt cgccttcgct atgggggcgc
    79801 tcttcctgct aagttactac accgacgtcg ctggcgtcgg tgccgctgcg gcgggcacca
    79861 tgctgttact ggtgcgggta ttcgatgcct tcgccgacgt ctttgccgga cgagtggtgg
    79921 acagtgtgaa tacccgctgg ggaaaattcc gcccgttttt actcttcggt actgcgccgt
    79981 taatgatctt cagcgtgctg gtattctggg tgccgaccga ctggagccat ggtagcaaag
    80041 tggtgtatgc atatttgacc tacatgggcc tcgggctttg ctacagcctg gtgaatattc
    80101 cttatggttc acttgctacc gcgatgaccc aacaaccaca atcccgcgcc cgtctgggcg
    80161 cggctcgtgg gattgccgct tcattgacct ttgtctgcct ggcatttctg ataggaccga
    80221 gcattaagaa ctccagcccg gaagagatgg tgtcggtata ccatttctgg acgattgtgc
    80281 tggcgattgc cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg
    80341 tacgtatcgt ggcgcagccg tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc
    80401 cgctgtttat gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg
    80461 cctcgtcgtt gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg
    80521 tactggtgca aaacctggtt ggtactgtgg catcggcacc gttggtgccg gggatggtcg
    80581 cgaggatcgg taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc
    80641 tgctattctt ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg
    80701 cttcaattgg tcagggcgtt accatgactg ttatgtgggc gctggaagct gataccgtag
    80761 aatacggtga atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat
    80821 ttacccgtaa atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttggggttaa
    80881 gcggatatat cgccaatcag gtgcaaacgc cggaagttat tatgggcatt cgcacatcaa
    80941 ttgccttagt accttgcgga tttatgctac tggcattcgt tattatctgg ttttatccgc
    81001 tcacggataa aaaattcaaa gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc
//
LOCUS       DAZGCQ010000006        79083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000006 DAZGCQ010000000
VERSION     DAZGCQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.006"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHK07_000448"
     CDS             complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHK07_000448"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO8100831.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            complement(1212..2405)
                     /locus_tag="ACHK07_000449"
     CDS             complement(1212..2405)
                     /locus_tag="ACHK07_000449"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO8100832.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHK07_000450"
     CDS             complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHK07_000450"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO8100833.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3073..3774)
                     /locus_tag="ACHK07_000451"
     CDS             complement(3073..3774)
                     /locus_tag="ACHK07_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8100834.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3790..4818)
                     /gene="gtdA"
                     /locus_tag="ACHK07_000452"
     CDS             complement(3790..4818)
                     /gene="gtdA"
                     /locus_tag="ACHK07_000452"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO8100835.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4830..6188)
                     /locus_tag="ACHK07_000453"
     CDS             complement(4830..6188)
                     /locus_tag="ACHK07_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8100836.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6315..7223
                     /locus_tag="ACHK07_000454"
     CDS             6315..7223
                     /locus_tag="ACHK07_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8100837.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7354..7752
                     /gene="yohJ"
                     /locus_tag="ACHK07_000455"
     CDS             7354..7752
                     /gene="yohJ"
                     /locus_tag="ACHK07_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO8100838.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7749..8444
                     /gene="yohK"
                     /locus_tag="ACHK07_000456"
     CDS             7749..8444
                     /gene="yohK"
                     /locus_tag="ACHK07_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO8100839.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPMILAVMG"
     gene            8574..9458
                     /gene="cdd"
                     /locus_tag="ACHK07_000457"
     CDS             8574..9458
                     /gene="cdd"
                     /locus_tag="ACHK07_000457"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO8100840.1"
                     /translation="MHPRFQTAFAQLAENLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            9608..10327
                     /gene="sanA"
                     /locus_tag="ACHK07_000458"
     CDS             9608..10327
                     /gene="sanA"
                     /locus_tag="ACHK07_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO8100841.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10330..10569
                     /gene="yeiS"
                     /locus_tag="ACHK07_000459"
     CDS             10330..10569
                     /gene="yeiS"
                     /locus_tag="ACHK07_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO8100842.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10763..12001
                     /gene="preT"
                     /locus_tag="ACHK07_000460"
     CDS             10763..12001
                     /gene="preT"
                     /locus_tag="ACHK07_000460"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO8100843.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCIAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11995..13230
                     /gene="preA"
                     /locus_tag="ACHK07_000461"
     CDS             11995..13230
                     /gene="preA"
                     /locus_tag="ACHK07_000461"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO8100844.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13473..14483)
                     /gene="mglC"
                     /locus_tag="ACHK07_000462"
     CDS             complement(13473..14483)
                     /gene="mglC"
                     /locus_tag="ACHK07_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO8100845.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14499..16019)
                     /gene="mglA"
                     /locus_tag="ACHK07_000463"
     CDS             complement(14499..16019)
                     /gene="mglA"
                     /locus_tag="ACHK07_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO8100846.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16080..17078)
                     /gene="mglB"
                     /locus_tag="ACHK07_000464"
     CDS             complement(16080..17078)
                     /gene="mglB"
                     /locus_tag="ACHK07_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO8100847.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17358..18398)
                     /gene="galS"
                     /locus_tag="ACHK07_000465"
     CDS             complement(17358..18398)
                     /gene="galS"
                     /locus_tag="ACHK07_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO8100848.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18540..19697)
                     /gene="yeiB"
                     /locus_tag="ACHK07_000466"
     CDS             complement(18540..19697)
                     /gene="yeiB"
                     /locus_tag="ACHK07_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO8100849.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDTQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFTQFDR
                     LGLLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19714..20382)
                     /gene="folE"
                     /locus_tag="ACHK07_000467"
     CDS             complement(19714..20382)
                     /gene="folE"
                     /locus_tag="ACHK07_000467"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO8100850.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20640..21476
                     /gene="yeiG"
                     /locus_tag="ACHK07_000468"
     CDS             20640..21476
                     /gene="yeiG"
                     /locus_tag="ACHK07_000468"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8100851.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21508..23487)
                     /gene="cirA"
                     /locus_tag="ACHK07_000469"
     CDS             complement(21508..23487)
                     /gene="cirA"
                     /locus_tag="ACHK07_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO8100852.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23779..25248)
                     /gene="lysP"
                     /locus_tag="ACHK07_000470"
     CDS             complement(23779..25248)
                     /gene="lysP"
                     /locus_tag="ACHK07_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO8100853.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25453..26334)
                     /gene="yieE"
                     /locus_tag="ACHK07_000471"
     CDS             complement(25453..26334)
                     /gene="yieE"
                     /locus_tag="ACHK07_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO8100854.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26433..27482
                     /gene="yeiH"
                     /locus_tag="ACHK07_000472"
     CDS             26433..27482
                     /gene="yeiH"
                     /locus_tag="ACHK07_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO8100855.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27556..28413
                     /gene="nfo"
                     /locus_tag="ACHK07_000473"
     CDS             27556..28413
                     /gene="nfo"
                     /locus_tag="ACHK07_000473"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO8100856.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28416..29504
                     /gene="yeiI"
                     /locus_tag="ACHK07_000474"
     CDS             28416..29504
                     /gene="yeiI"
                     /locus_tag="ACHK07_000474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8100857.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29560..30810)
                     /gene="nupX"
                     /locus_tag="ACHK07_000475"
     CDS             complement(29560..30810)
                     /gene="nupX"
                     /locus_tag="ACHK07_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8100858.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     MLSPATESSQVSFNNLSFTETPPKSIIEAAATGAVTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30910..31851)
                     /gene="rihB"
                     /locus_tag="ACHK07_000476"
     CDS             complement(30910..31851)
                     /gene="rihB"
                     /locus_tag="ACHK07_000476"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO8100859.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            31981..32679
                     /gene="yeiL"
                     /locus_tag="ACHK07_000477"
     CDS             31981..32679
                     /gene="yeiL"
                     /locus_tag="ACHK07_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO8100860.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32747..33997)
                     /gene="psuT"
                     /locus_tag="ACHK07_000478"
     CDS             complement(32747..33997)
                     /gene="psuT"
                     /locus_tag="ACHK07_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8100861.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34091..35029)
                     /gene="psuG"
                     /locus_tag="ACHK07_000479"
     CDS             complement(34091..35029)
                     /gene="psuG"
                     /locus_tag="ACHK07_000479"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO8100862.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(35017..35958)
                     /gene="psuK"
                     /locus_tag="ACHK07_000480"
     CDS             complement(35017..35958)
                     /gene="psuK"
                     /locus_tag="ACHK07_000480"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO8100863.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQSAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNLIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36382..38073)
                     /gene="fruA"
                     /locus_tag="ACHK07_000481"
     CDS             complement(36382..38073)
                     /gene="fruA"
                     /locus_tag="ACHK07_000481"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO8100864.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVASLAYAFLKRPEVDAVAKAA"
     gene            complement(38090..39028)
                     /gene="fruK"
                     /locus_tag="ACHK07_000482"
     CDS             complement(38090..39028)
                     /gene="fruK"
                     /locus_tag="ACHK07_000482"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO8100865.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39028..40158)
                     /gene="fruB"
                     /locus_tag="ACHK07_000483"
     CDS             complement(39028..40158)
                     /gene="fruB"
                     /locus_tag="ACHK07_000483"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO8100866.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40526..41707
                     /gene="setB"
                     /locus_tag="ACHK07_000484"
     CDS             40526..41707
                     /gene="setB"
                     /locus_tag="ACHK07_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO8100867.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41704..41958)
                     /gene="yeiW"
                     /locus_tag="ACHK07_000485"
     CDS             complement(41704..41958)
                     /gene="yeiW"
                     /locus_tag="ACHK07_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8100868.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42113..42685
                     /gene="yeiP"
                     /locus_tag="ACHK07_000486"
     CDS             42113..42685
                     /gene="yeiP"
                     /locus_tag="ACHK07_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO8100869.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42908..44374
                     /gene="yeiQ"
                     /locus_tag="ACHK07_000487"
     CDS             42908..44374
                     /gene="yeiQ"
                     /locus_tag="ACHK07_000487"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8100870.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            44492..45478
                     /gene="yeiR"
                     /locus_tag="ACHK07_000488"
     CDS             44492..45478
                     /gene="yeiR"
                     /locus_tag="ACHK07_000488"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO8100871.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNGLQSALLKLRLATTA"
     gene            45517..46230
                     /gene="lpxT"
                     /locus_tag="ACHK07_000489"
     CDS             45517..46230
                     /gene="lpxT"
                     /locus_tag="ACHK07_000489"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO8100872.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFSDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46642..47208
                     /gene="mepS"
                     /locus_tag="ACHK07_000490"
     CDS             46642..47208
                     /gene="mepS"
                     /locus_tag="ACHK07_000490"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO8100873.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47389..48945
                     /gene="pdeN"
                     /locus_tag="ACHK07_000491"
     CDS             47389..48945
                     /gene="pdeN"
                     /locus_tag="ACHK07_000491"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8100874.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49027..50841
                     /gene="yejA"
                     /locus_tag="ACHK07_000492"
     CDS             49027..50841
                     /gene="yejA"
                     /locus_tag="ACHK07_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO8100875.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSAKQQGE"
     gene            50842..51936
                     /gene="yejB"
                     /locus_tag="ACHK07_000493"
     CDS             50842..51936
                     /gene="yejB"
                     /locus_tag="ACHK07_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO8100876.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51936..52961
                     /gene="yejE"
                     /locus_tag="ACHK07_000494"
     CDS             51936..52961
                     /gene="yejE"
                     /locus_tag="ACHK07_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8100877.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52963..54552
                     /gene="yejF"
                     /locus_tag="ACHK07_000495"
     CDS             52963..54552
                     /gene="yejF"
                     /locus_tag="ACHK07_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO8100878.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54556..54900)
                     /gene="yejG"
                     /locus_tag="ACHK07_000496"
     CDS             complement(54556..54900)
                     /gene="yejG"
                     /locus_tag="ACHK07_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO8100879.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55233..56423)
                     /gene="bcr"
                     /locus_tag="ACHK07_000497"
     CDS             complement(55233..56423)
                     /gene="bcr"
                     /locus_tag="ACHK07_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO8100880.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56451..57146)
                     /gene="rsuA"
                     /locus_tag="ACHK07_000498"
     CDS             complement(56451..57146)
                     /gene="rsuA"
                     /locus_tag="ACHK07_000498"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO8100881.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57295..59055
                     /gene="radD"
                     /locus_tag="ACHK07_000499"
     CDS             57295..59055
                     /gene="radD"
                     /locus_tag="ACHK07_000499"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8100882.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAELRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59180..59464
                     /gene="rplY"
                     /locus_tag="ACHK07_000500"
     CDS             59180..59464
                     /gene="rplY"
                     /locus_tag="ACHK07_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO8100883.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59603..60610)
                     /gene="yejK"
                     /locus_tag="ACHK07_000501"
     CDS             complement(59603..60610)
                     /gene="yejK"
                     /locus_tag="ACHK07_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO8100884.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60792..61019
                     /gene="yejL"
                     /locus_tag="ACHK07_000502"
     CDS             60792..61019
                     /gene="yejL"
                     /locus_tag="ACHK07_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO8100885.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61039..62799
                     /gene="yejM"
                     /locus_tag="ACHK07_000503"
     CDS             61039..62799
                     /gene="yejM"
                     /locus_tag="ACHK07_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO8100886.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFITFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62874..62950
                     /locus_tag="ACHK07_000504"
     tRNA            62874..62950
                     /locus_tag="ACHK07_000504"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62908..62910,aa:Pro,seq:ggg)
     gene            complement(63052..65643)
                     /gene="yejO"
                     /locus_tag="ACHK07_000505"
     CDS             complement(63052..65643)
                     /gene="yejO"
                     /locus_tag="ACHK07_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO8100887.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65963..66610
                     /gene="narP"
                     /locus_tag="ACHK07_000506"
     CDS             65963..66610
                     /gene="narP"
                     /locus_tag="ACHK07_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO8100888.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66645..67697)
                     /gene="ccmH"
                     /locus_tag="ACHK07_000507"
     CDS             complement(66645..67697)
                     /gene="ccmH"
                     /locus_tag="ACHK07_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO8100889.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67694..68251)
                     /gene="dsbE"
                     /locus_tag="ACHK07_000508"
     CDS             complement(67694..68251)
                     /gene="dsbE"
                     /locus_tag="ACHK07_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO8100890.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68248..70191)
                     /gene="ccmF"
                     /locus_tag="ACHK07_000509"
     CDS             complement(68248..70191)
                     /gene="ccmF"
                     /locus_tag="ACHK07_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO8100891.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            complement(70188..70667)
                     /gene="ccmE"
                     /locus_tag="ACHK07_000510"
     CDS             complement(70188..70667)
                     /gene="ccmE"
                     /locus_tag="ACHK07_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO8100892.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70664..70873)
                     /gene="ccmD"
                     /locus_tag="ACHK07_000511"
     CDS             complement(70664..70873)
                     /gene="ccmD"
                     /locus_tag="ACHK07_000511"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO8100893.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRSVAQQRAREARLRAAQQQEAA"
     gene            complement(70870..71607)
                     /gene="ccmC"
                     /locus_tag="ACHK07_000512"
     CDS             complement(70870..71607)
                     /gene="ccmC"
                     /locus_tag="ACHK07_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO8100894.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71649..72311)
                     /gene="ccmB"
                     /locus_tag="ACHK07_000513"
     CDS             complement(71649..72311)
                     /gene="ccmB"
                     /locus_tag="ACHK07_000513"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO8100895.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72308..72931)
                     /gene="ccmA"
                     /locus_tag="ACHK07_000514"
     CDS             complement(72308..72931)
                     /gene="ccmA"
                     /locus_tag="ACHK07_000514"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO8100896.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(72944..73546)
                     /gene="napC"
                     /locus_tag="ACHK07_000515"
     CDS             complement(72944..73546)
                     /gene="napC"
                     /locus_tag="ACHK07_000515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO8100897.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73556..74005)
                     /gene="napB"
                     /locus_tag="ACHK07_000516"
     CDS             complement(73556..74005)
                     /gene="napB"
                     /locus_tag="ACHK07_000516"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO8100898.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(74002..74865)
                     /gene="napH"
                     /locus_tag="ACHK07_000517"
     CDS             complement(74002..74865)
                     /gene="napH"
                     /locus_tag="ACHK07_000517"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO8100899.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74852..75547)
                     /gene="napG"
                     /locus_tag="ACHK07_000518"
     CDS             complement(74852..75547)
                     /gene="napG"
                     /locus_tag="ACHK07_000518"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO8100900.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75554..78040)
                     /gene="napA"
                     /locus_tag="ACHK07_000519"
     CDS             complement(75554..78040)
                     /gene="napA"
                     /locus_tag="ACHK07_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO8100901.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78037..78300)
                     /gene="napD"
                     /locus_tag="ACHK07_000520"
     CDS             complement(78037..78300)
                     /gene="napD"
                     /locus_tag="ACHK07_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO8100902.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNASPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78290..78784)
                     /gene="napF"
                     /locus_tag="ACHK07_000521"
     CDS             complement(78290..78784)
                     /gene="napF"
                     /locus_tag="ACHK07_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO8100903.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            78893..79057
                     /locus_tag="ACHK07_000522"
     CDS             78893..79057
                     /locus_tag="ACHK07_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100904.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT        19275 a        21334 c        19580 g        18894 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagttca tgaaaggagc gcaacaagat gatgaagctg
       61 aataagcgat tcattgtctc tgataaactg cgattccact attatcgcag cttatcgata
      121 tcaattgcct gaatagccct tgcaatatca tgggaattat tcaacacccg aacatgctga
      181 aataattccg ttgcttcatc gtattcttta cgcaaataac tcaaccactg tttaatccgc
      241 gctacgtgat ataacccggt atcgccctgc ttttccagac gggtatattt ttgcagcaaa
      301 gcaaccacct ccggccacgg cattcgcggt tcgttatatt ttaccacccg gctcaggttg
      361 ggaatattga gcgccccgcg accaatcatc actgcgtcgc agccgctgat cgccatgcat
      421 tgttgcgcgc tctgccagtc ccagatttca ccgttggcaa tcaccggaat attcagccgc
      481 tggcgaatct caccaatcgc ctgccagtca atatgctccg cgcggtaacc ctgctctttt
      541 gtccgtccat gcaccaccag ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca
      601 aatttcttct caccgctgtc ccagcccaga cgcactttca cgctgacggg caaatgcgcc
      661 ggtacagctt cacgcatcgc ttttgcaccc tggtagatga gttcaggatc tttgagtaac
      721 gtcgccccac caccgctacc gttgaccgtt ttcgacgggc agccgcaatt gagatccacg
      781 ccccaggaac ctaattccac cgcacgggcg gcgttctctg ccagccattg tgggaactga
      841 cctaacaact gaacgcgcac cagcgtaccg gatggtgtcc ggctggcgtt ttgtaactca
      901 gggcaaatgc gatgaaagac ttttaccggc agcagttgat ccaccacgcg gacaaactcg
      961 gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt cacgcaccag agagtcaagc
     1021 acaccctcca tcggtgccag taacacacgc atatcatcac ccgcaaaaaa atgaggcgct
     1081 atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact gattaattcg ggcaagattt
     1141 ttaccctcac cctaaccctc tcccgaaggg gcgaggggac cgcccgtgcg cctgattggg
     1201 tgaagaagtg ctcagcgtgg ttgcaaacag ttatcttctt tccagccgta caaccactcc
     1261 atgccgcgat aaaattctgc ctgcgatttc cctttccaca gcagattacg tacctggcgt
     1321 tctacccctg cggcgtggta aacccgcccc atttcacggg tcgaccagac gatgcgcgcg
     1381 gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggcct gcgccgcatc accctcacac
     1441 tgcgccagcg ccttacccag cgttactgca tcttccagcg ccatacaggc tccctgcgcc
     1501 atatattgcg ccaccggatg ggccgaatcg cccacaagcg ttatgcgctt cgttccccac
     1561 ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc atgaagtcgg tttatccaac
     1621 atctggcgcg gacgggggtg aatcccttca aagtaagaga gcacttcctc tttactacca
     1681 tccttcacgc cccactcttc ctgctggcgg ctgtggaatg ttaccaccag gttatactgc
     1741 ttgccgccgc gcaaagggta atgcaccaag tgacaatgcg gccccgccca cagtaccggt
     1801 gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga taaccgcacg gtagacaaca
     1861 tgtccggtga cacgcggcgc atcgcccaat aagctctggc gcaccactga cttcacgccg
     1921 tcacagccaa tcagaatatc gccggtccag ctattgccgc gatcgtcaaa cacggtgaca
     1981 tcgtcttccg tctcgcgaat atccacgacg ttggtggagg tgtgatattc cacgtccgga
     2041 tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa cccggtggat caccgcgtaa
     2101 gggccgccga aatggtcacg aaaagcctgc ccggtttcaa tgcacaccac ttcttcggcg
     2161 ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg cgcgctggcg ggcaacttcg
     2221 ccgacgccga ggctgtccag cgccgaaaac gcatttggcc ccaactggat gcccgcgcca
     2281 atctcaccga tctcatgcgc cttttccagc agcaccactt tgatccccag gcgggccagt
     2341 gacagcgcag ttgccgcacc gccgatcccg cctcccacaa ttactgcgcg tgtcactttc
     2401 gtcattatag ttctcctggt caggctgaaa ttttatcttg ttgattttct ggcgctgcgg
     2461 caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc tttgcagcgt ggatacccgc
     2521 tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg gacaaggcag caatctgcca
     2581 gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt ctggcgcaac agttgttcca
     2641 ccgcgcttaa cccttgctgg atccagtgcg cataccaacg ttttttttct tcttcactga
     2701 cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt attcaatgga tgaatatcac
     2761 aagcgatggc atagacaatt tccagcacct tgttgcgggc cggctcttcc tgcggcacca
     2821 gcggaacttg cgggtagtgc ctctccagcc agtcaataat cgccagcgat tgtccgagtg
     2881 attcgccctc gtcagtcagc agcgtcggca ccagcccgac cggattcatc cgccgatagg
     2941 ccagttcatt ttgctgacca atacggatat tcaccccttc ggtctggtaa ttaatgcctt
     3001 tcagcgccag cgcaatgcgt acccggtacg acgctgaact attaaaaaaa ctgtacagct
     3061 tcatcatgca cctcagacaa tcttcaccgc gataggcgtt aagccctcga cgttgcctgt
     3121 gatcacatca cctttcacta ccgggccaac cccttccggc gtaccggtga agatcagatc
     3181 gcccggttgc agctcaaaga agcctgacag atagctgatg gtttcattca ccgaccagat
     3241 gagatggcga atatcgctgc gctgatgatc ttcgccgtta acctgtaacc agattggcgc
     3301 attatcaata ccgccgatat cgcgggcttt gtgcagcggc gcgatgggcg ctgagagatc
     3361 aaacgcttta ccgatttccc acgggcggcc catctggcgc atttccattt gacggtcacg
     3421 ccgagtcata tcaagacccg tggcataacc ccagacatat tcatgggcgt gttccagcgg
     3481 gatatcgctg ccttttttac caatcgccac caccagttca atttcgtaat ggtagttatc
     3541 ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg gcaaccggaa caacggcatc
     3601 cgccggtttg cagaagaaga atggcggttc acgatccgga tcaaagccca tttcgcgggc
     3661 gtgagcggca tagttacgtc cgacgcagta aacgcggcgc acggggaatt gttcatcact
     3721 gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat acgtattgag tcattttttc
     3781 tctcctaaat taataacgcg cttcgcggaa cagccccagg gcttcttgta ccggtctgtc
     3841 cgaaaaacta aataaaacgg aatcttgcgt ggtctggaac gacacgccat gccaggtcgg
     3901 caccacgaag atatcttttg cactaaagct gaaggtttca ttgccgatga tgacctgccc
     3961 gctaccttcc accacatggt agatggtgct gtcagtggta cgcgcaacgc gtgaggcaaa
     4021 ccctttcggc agcagttgca ggaaagcgcc catcgacggc atcggatagc cgccggtgac
     4081 cgggttgacg tagcgcattt tgtagccatc ccactcgtcg gcatcgccca gtcgggttaa
     4141 atcgtgcagc acttcgcggc tgcggtcata acgatagtta aagatcggcg aggagttccc
     4201 cgtctgatgc cgcagcggca acatattggc agcgtaacgc ggcagataat ctccctcttt
     4261 acgcgttacc ggttgttgct cttccggata atcttcggca aaaccgcagc ccagaatatt
     4321 cactaatggc agatccagcc catcgagcca gataaccggt tcgtcgccag ggttaccgtg
     4381 atcgtgccag cgccactgcg gggtcaggat aaaatcgcct tcattcattg gcgtgcgttc
     4441 gccatcaacg gcggtaaatg cccctttgcc ttcaacaata aaacgcagcg ccgactgatt
     4501 atgacgatga ctcggtgcca cttcgcccgg catgatcagt tgcaaaccgg catataaagt
     4561 cgcggtaatg gaagactgcc cgcgcagcgc cgggttttcc agcaccagca cgcggcgcac
     4621 ggcctctttc gcaccaatca ggccgccgct ctccagtagc agcgggcgga tctcctgata
     4681 attccagtac gctggcgcac agttagcgtt gggcgttttc ggcaccagat ggtgcagtga
     4741 ctcccacagc ggggtcaggt tttgcccgga aatatgctga taaaattgct cgcggctatt
     4801 ttgattgtta tcagtcattg ggatgctcct tattcgttca caacgcctgc gcgaggcagt
     4861 gagtcaggga cagaggtcga ctggcgaacc acggtaatca gcagggttaa catcaccgca
     4921 ctgatcgccg caggcacagc gataatgaaa aacagggtgt cgaaagagaa attcatcgcc
     4981 atcatcacgc cgccagacag cgaaccaaca atcgcgccgc agcggccgat agcatttgac
     5041 cagctcacgc ccgtggcgcg actttgtgtt ggataaagtg ttgcggtcag cgcattcagc
     5101 cccacctgcg atccgctaat gccaatgcca gtgccgaaaa tcgccagcgc catcaaccat
     5161 aatccatcct ggcttaagcc aatcatcacg atgcatatag cgcctatggc atagctcagc
     5221 gccagcacgc ggaaaggatt gaacttatcc atcagcacac ccagcgccag cgcgcctaac
     5281 gtaccaccaa tctggaacgc ggcggtgacc caggacgcgt gttgcaggtc gatgccgcga
     5341 tggttcagta gcgtcggcat ccagcttgag agcaggtaaa taatcagcag gctcataaag
     5401 aacactaccc acagcatcaa cgtgatgggt aactgacgtc ccataaacag ttgacgaatg
     5461 ctgccttttg taacggaggc tgattcaatg agataaaagt gggtatcgac ataacgctcg
     5521 cgggtgatgg cgctgacggt tttcgcaatc accgcctgcg gtagctggcg gcgcacctgc
     5581 cagcgtggcg attccggcag aactaccagc agtgcgacaa acagcatcag cggtaacacg
     5641 ccccccaaca ccagaatgcc gtgccagcca atcaccggca ccagctgcgc actgacaatc
     5701 ccgccaaacg ccgaacccag cgtaaagccg caaaacatca gcgtcaccag cgcgccgcgg
     5761 cggcgggcag gcagatactc cgaggtcatg gtgatggtgt tcggcatcgc gccgcccaga
     5821 cccaggccgg tgagaaagcg caggaagacc agcgtttgca gatccggtga gaaagccgag
     5881 gcgaggctta acgcgccaaa caaaaagacg cacagctcaa tcacccgctt gcgaccaaag
     5941 cgatcggaca acgggccaca aagcagtgcg cctgcggtta atcctagcag acccgcgcca
     6001 aaaagcgggg cgagatcgcc tgcggtgagt tgccagtgag tgcgaatatc aggggcgata
     6061 aatccgatag ccgcggtgtc aaaaccatcg agcattacca ccagaaaaca gcagatgata
     6121 acgcgccact gcatcttgcc gaccggagca gcatcgataa gtgcttgtaa atcacgtcgt
     6181 tgagtcatag taaatgcctc agtgcaggta aggtagatgt ttcttttgtt ttgtatatgt
     6241 tgcgatattg tgattaaaga gatgagatgt acaatggcat ttcgggcaga gagataacct
     6301 ggaggttatg gcaaatggcg aactgggcgc aaaaattaaa actacatcac cttgaaatgc
     6361 tggtggcgct gggtgaacag ggcaatctaa cccatgtcgc acgaatgatg aatatcaccc
     6421 aacctgcgct ttcgaaatgg ttgtcacaac tcgaagatga gattggaatc acactttttg
     6481 agcggcacag caaaggatta cgtccgactg aaggcggcaa attgttattg caacatgccc
     6541 aacggttaat caatgacatg gagcgatcgc aacatgaact ggcccgtttt aaacaaggtg
     6601 gactggtcgg cagtctgaaa attggctgtt ctcccgtcgc cactgactgc gtatcccagg
     6661 cgattttgca tttgcttacg aagatgccaa ctctacacct gaatatcgaa gagaaggtga
     6721 tgaccccatt gctgcaagat ctattggcag gttctgtcga tgtggtcgtt gggcgaattg
     6781 gcggtcgcgc attgcagctg ccactgaatt atcaggtgct gtacaccgag ccagtatgct
     6841 ttgtcgcccg ccctgagcac ccactggcga aatacgccac cctcagctgg aatgatcttg
     6901 ccaactggcg ctggatcgtc tggccgacgg gtacccccat tcgcattagt attgataatg
     6961 cgctggtaga ccacggcgta atgctgccag aaaacacgat tgagtcggcg tcgatgaacg
     7021 tcagtaccaa cctgttacaa agcagcgata tgatctctat tttgtcatta cgcctggcgc
     7081 aacgttatgc cagccaggga caactggtga tactcaacct gccaaagata gaacagaaag
     7141 gtagcgtcgg agtgttctgg cgcaataacg aaacgccttc ttatgcttta agtcagtttc
     7201 tgcacttttt acagcagaat tagtgtgctg aacggggcgc tcgcccgagg ttacctccct
     7261 aaaattcaac tatgctgaga attcttaatg tgatcggtag cacgttttac gaattaattg
     7321 tatgatgaat ccatctcatc tggggtgttg attatgagta agacactgaa cattatctgg
     7381 caatatttac gcgctttcgt cctgatttat gcctgcctgt atgcaggcat tttcattgct
     7441 tccctgctac cggtaaccat tccgggcagc atcatcggga tgctgatcct gtttgtcctg
     7501 ctggccttgc aaattcttcc ggcgaaatgg gtcaatccgg ggtgctacgt actgattcgc
     7561 tatatggcgc tattgtttgt gccgattggc gtaggcgtca tgcaatattt tgatttgctc
     7621 cgcgcacagt ttggcccggt agtggtttcc tgtgcaatca gtacgctggt ggtttttctg
     7681 gtggtgagct ggagttcgca actggtacac ggtgaacgta aagtcgtagg tcagaaagga
     7741 tcagaagaat gatggcgaat atctggtggt cattaccgct gacgttgatt gtcttttttg
     7801 ccgcccgcaa actggcggca cggtataagt ttcccctgct taacccgttg ctggtagcaa
     7861 tggtggtgat cattcctttt ttaatgctga ccggcatctc ttacgacagt tactttaaag
     7921 gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt cgcgctggcc tatcctttat
     7981 atgagcagct acaccagatc cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg
     8041 gcagcgtggt tgcgatggtg acggggactt ccgtggcatt gttgatgggc gcttcaccgg
     8101 aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc tattgcaatg gcggttggcg
     8161 gcagtattgg cggtattccg gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg
     8221 gcgcggtatt tggccataca ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg
     8281 gtctggcaat ggggactgcc tcgcacgccc tcggtacagc gcgctgcgcc gagctggatt
     8341 atcaggaagg tgcatttagt tcgctagcgc tggtgttatg cgggataatt acttcgctga
     8401 tcgcaccgtt ccttttcccg atgattctgg cagtaatggg ctaaaatttg cgatgcgtcg
     8461 cgcatttttg atgtaagttt cacgcgttgc ataattaatg agattcagat cacatataaa
     8521 gccataacgg gttcgtaaac tgttatccca ttacatgatt atgaggcaac gccatgcatc
     8581 cacgttttca aaccgctttt gcccaacttg cggaaaactt gcaatctgca ctggaaccta
     8641 ttctggcaga caagtacttc cccgctttgt tgaccgggga gcaagtctca tcgctgaaga
     8701 gcgcaacggg gctggacgaa gacgcgctgg cattcgcact acttccgctg gcggcggcct
     8761 gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa
     8821 cctggtattt tggtgccaat atggaattta ttggtgcgac aatgcagcaa accgttcatg
     8881 ccgaacaaag cgcgatcagc cacgcctggt tgagtggtga aaaagcgctt gcagccatca
     8941 ccgttaacta cacgccttgt ggtcactgcc gtcagtttat gaatgaactg aacagcggtc
     9001 tggatctgcg tattcatctg ccgggccgcg aggcacacgc gctgcgtgac tatctgccgg
     9061 atgcctttgg gccgaaagat ctggagatta aaacgctgct gatggacgag caggatcacg
     9121 gctatgcgct gactggtgat gcgctttctc aggcggcgat tgcggcggca aaccgttcgc
     9181 acatgcctta cagtaagtcg ccaagcggtg ttgcgctgga atgtaaagac ggtcgaattt
     9241 tcagcggcag ctacgctgaa aacgccgcat tcaacccgac tctgccgccg ttgcagggag
     9301 cgttaattct gttgaatctc aagggttatg attacccgga tatccagcgc gcggttctgg
     9361 cagaaaaagc cgatgcgccg ttgattcagt gggatgccac ctccgcaacg ctgaaagcgc
     9421 tcggctgtca cagtatcgac cgcgtgcttc tcgcttaagc ctggtgccgg atgcggcgtg
     9481 aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa aatcccggca aacagtttgc
     9541 cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg aaattttcct tagatcgagt
     9601 ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc cttatcggct tgctgctgtt
     9661 gactgtgctc ggcctcgatc gctggatgag ctggaaaacc gcgccttata tctacgacga
     9721 attgcaggat ctcccctacc gccaggtcgg tgtggtgctc ggaacagcaa aatattatcg
     9781 tactggcgta attaatcagt attatcgcta ccgcattcaa ggagcgatta atgcctataa
     9841 cagcggtaag gtaaattatc tattactgag cggcgataac gcattgcaaa gttataatga
     9901 gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc gacccatcag atattgttct
     9961 cgattacgca ggctttcgta cgctggattc catcgtgcgt acacgcaaag ttttcgatac
    10021 taatgatttc attattatca cccaacgttt ccactgtgag cgggcattat ttattgcgct
    10081 gcatatgggg attcaggctc agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt
    10141 acgtattcgc gaatttgccg cccgtttcgg tgcgctggct gacctttata tttttaaacg
    10201 tgaaccgcgt tttttagggc cgctggtccc tattccggct atgcaccagg tgccggaaga
    10261 tgcgcagggg tatcccgccg tcacacccga acagttactt gaattacaaa agaaacaagg
    10321 aaagtagtta tggatgtaca gcagtttttt gtcgttgccg tttttttcct tatcccgata
    10381 ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa
    10441 aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc ccggactctt tttcgcttat
    10501 atggtggcat atatcggctt cggaatttta tctatcggca tgattgttta tcttattttc
    10561 tatcgttaat attttctctt ttctgttaat tattgagaat aattattact tcactcaata
    10621 agctgcggat ataacattct gaaccgcagc tatttgtgaa tcctttcaca gtttaaattt
    10681 ccccacgcgc atagccttaa tatcagcacg caatatttat ttagcgttcg ccttaattac
    10741 tcatagcatt aaggaagatc acatgccgca acaaaattat ctggatgaac tcactccggc
    10801 ttttacgcct ttactggcga ttaaagaagc ctctcgctgt ttattatgtc acgacgctcc
    10861 ctgtagtcag gcttgcccgg cacagaccga tccggggaaa tttattcgct caatctactt
    10921 tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat aatgccctcg gtgccgtttg
    10981 tgccagagtg tgcccgacgg agaaattatg ccaaagcggt tgtacccgtg ccggtgttga
    11041 tgctcccatt gatatcggcc gcttacagcg ttttgttact gattttgaac aacaaacagg
    11101 gatggaaatt tatcagcctg gtactaaaac gctcggcaaa gtcgcgatta ttggcgcagg
    11161 tcctgccgga ttgcaggcca gtgtgacact gacaaaccag ggttatgacg tcacgattta
    11221 tgagaaagaa gcgcaccccg gtggctggtt gcgtaacggt attccgcaat tccggttacc
    11281 gcaatcagtg ctggatgcag agatcgcccg tattgaaaaa atgggcgtga ccattaagtg
    11341 caataacgaa atcggtaaaa cactcaccct ggagcaactg aaagcggaaa accgcgcggt
    11401 actggtcacc gtggggttat caagcggttc cgggctaccg ctgtttgagc atagcgacgt
    11461 tgagattgcc gtcgacttcc tgctacgtgc acgacaggcg caaggcgata tcagcattcc
    11521 acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg gacgtagcca gcacgctgaa
    11581 agttctcggc tgtcaggcgg taacctgcat agcgcgtgaa gagttagatg agtttccggc
    11641 aagcgaaaaa gagtttgcca gcgcccggga gctgggcgtt tcgatcatcg atggattcac
    11701 gccagtagcc gtggaaggca ataaagtcac gtttaagcat gtacggttat cgggcgaact
    11761 gacgatggtg gcagataaaa ttattctcgc cgtcggtcag catgccagac tggatgcctt
    11821 tgcggagtta gagccgcagc gtaacaccat caaaacacaa aattatcaga cccgcgatcc
    11881 gcaagtcttt gctgctggcg atattgttga gggtgacaaa accgtggtct atgccgtgaa
    11941 aaccgggaaa gaagccgccg aggcgattca tcactattta gagggagctt gctcatgtta
    12001 acgaaagatc tttcgattac tttttgcggc gtgaagtttc ccaacccgtt ctgcctctct
    12061 tcttcgccgg taggcaactg ctatgagatg tgtgccaaag cctacgacac aggttggggc
    12121 ggcgtggtgt ttaaaacgat cggctttttt atcgccaacg aagtctcgcc gcgttttgat
    12181 catctggtga aagaagatac cggttttatc ggcttcaaaa atatggagca gattgctgaa
    12241 catccgttgg aagagaatct ggccgccctg cgtcggctga aggaagatta cccggacaaa
    12301 gtattgatcg cttcgatcat gggggaaaat gagcagcaat gggaggagct ggcgcgcctg
    12361 gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct cctgtccgca aatgacttct
    12421 catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg tagaaaaata ttgtcgggca
    12481 gtgaaacggg gttccacgct gccaatgctg gcgaagatga cgccgaatat cggtgatatg
    12541 tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg gcattgcggc gattaacacc
    12601 gttaaatcca tcaccaatat cgatcttaat cagaaaatcg gtatgccgat cgttaacgga
    12661 aaatcgagta tttccggata ttccggtaaa gcggtaaaac cgatcgccct gcgcttcatt
    12721 cagcaaatgc gcacccatcc agaactgcgc gatttcccaa tcagcggtat cggcggcatt
    12781 gaaacctggg aggatgcggc ggagttttta ttgctcggcg cagcaacgtt acaggtgacc
    12841 accggcatta tgcagtacgg gtatcggata gtggaagata tggcgagcgg gttgtcgcat
    12901 tatctcgccg atcagggatt tgattcgttg caggagatgg taggtctggc gaataacaat
    12961 attgtcccgg cggaagattt agaccgcagt tatattgtct atccccgtat caatcttgat
    13021 aaatgtgttg gctgtggacg ctgttatatt tcctgttacg acggcggtca ccaggcgatg
    13081 gaatggagcg agaaaacccg cacaccgcat tgtaataccg agaaatgtgt gggttgtctg
    13141 ctttgtggtc acgtctgccc ggtgggttgt attgagctcg gggaagtgaa gtttaagaaa
    13201 ggcgagaaag aacacccggt aacgttgtaa aaccagagac gcatccggca tttggttcct
    13261 gtcgccggat gcggcttgaa cgccttatcc ggcctacaaa accatacaaa tcagtatgtt
    13321 gagatcattg taggccggat aaaacgcgcc agcgttgcat ccggcatttg gctcctgtcg
    13381 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaacca cacagatcat gatgttatgt
    13441 gcgtttatga ccgaatgcgg accacaatca tatcacttct tacgcgcgta cttcagtgaa
    13501 tccagcgcta cggcgaagat aataatcgcc cctttgatga tgtactgcca gtatgggttt
    13561 acgccgatat acgtcaggcc atagttgatg acggtaaaaa taattacccc ggtcaccacg
    13621 ccaatcaccg tccccacacc gccgctgaac gatacgccgc ctaccacgca cgccgcgata
    13681 gcatccagct catacataaa gccgaggttg ttggtggcag agccgatacg tccggcttct
    13741 aacatcccgc caaaggcata gaacacgcca gacaacgcgt agatcatcag caggttcagg
    13801 ccgacgttga caccagatac ttttgccgct tccgggttac cgccaatggc aaaaatgttc
    13861 ttaccgaagc gggttttgtt ccacaacacc cagacgaacg ccaccgcaat caacgcgtag
    13921 aaggtgatgt aagagagacg gaaactcccc agcgcgacaa agccctgagc aaaggtagag
    13981 aagccactgt caaaaccaga aattggcgac gccccgacga aatcgtaata gagcgagttg
    14041 atgccataga cgatgatcat cgtgccgagc gtggtaatga acggcgtaac gttgagataa
    14101 gcgataatca gaccgttgat caaaccgatc accgcaccaa tggcacagac aatcagaata
    14161 accagcgcaa tcggcatcgt cgccatttcc gggaacactt tgttggcgtt atccatggac
    14221 tgcaataatg tcgctgccac cactgccgcc agccctactt gacgccccgc agaaaggtcg
    14281 gtcccctggg tgacaattaa ccctgccaca ccgagcgcga taataatacg caccgatgac
    14341 tgggtgagaa tattacttaa gttcaacaga cttaaaaatg ttgggtcctg gaaaataata
    14401 atcgccagca aaactaaaag aacgacgtaa ataccgccct ctttcaggta agtaagaaaa
    14461 cttttcttat ttaacgcact catgggaagc ccctaatctt aaaggtgcaa agacgcaaga
    14521 cgcagaattt cgttttgcgt tgttgttttt gtatcgacaa ttccggaaac gagaccattg
    14581 ctcatgacca gaatacggtc tgttatccct aacaactcag gcatttcaga ggagataata
    14641 ataatccctt tgcctttctt cgccagttcg gcaattaact gatagatctc aaacttcgcc
    14701 ccgacatcaa taccgcgcgt cggttcatcg agcattaata tttccggttg cgttagtagc
    14761 cagcgcccaa taatcacctt ttgctgatta ccaccggaga gcgaaccaat ttgcgtccga
    14821 tgccccggtg tttttacccg catcgaatca atcacccact gggtatcgct tttcatccgc
    14881 gagttatcca gtaaaccaac tttatttttg tagttgcgaa tattggaaat taacgagtta
    14941 aaaccaatat ccagataggc ataaattcct gttgagcggc gctcctcagt taccagtgca
    15001 aatccgtggt ttatggcttc gttggcatta tggttattga tctttttgcc gtgcaacgta
    15061 atggtgccag ccgatttctc gcgaatacca aataacgtct caacaatatc ggtacgtttc
    15121 gcccccacca gaccggcaat accgaggatc tcccctttat gcagatcaaa cgagacatca
    15181 cggatcgacg gctggcgcag tgacgtcagg ttacgcacct cgaggatgac ttcgcccggc
    15241 ttgttttctt tgtcagggaa acgctggtta agagaacgcc caaccatcat ggcgatgatc
    15301 ttatccatcg tcagtcctgc cagcggttcg gtggcgatcc actgaccgtc gcgcaatacg
    15361 gtaacttcat cacataactg gaagatttct tccattttat gcgagatata aacaataccg
    15421 cagccgcgct cttttaattt acgaataata gtgaacagat gattgacctc tttttcggtt
    15481 aacgaagaag tcggttcatc cataatcaca attttcgcgt tataggaaaa cgctttggcg
    15541 atttcgatca tctgcatttg cgaaacagat aatgtgccga cgcgcgcacg cggatcgata
    15601 tcaatatcca gttcatcaaa aatcgctttg gtttcgcggt acattttgtc ctgatcgaca
    15661 aacatgcctt tggtgggata tcgccccagc cacatgttat ccatcaccga acgttgtaat
    15721 accaggttta actcctggtg taccatcgaa ataccatttt ccagggcttc tttggccgaa
    15781 tggaaatcga tctctttacc ctggaataaa atggtgccgg agtctttttg ataaatacca
    15841 aacaggcatt ttaataatgt cgattttcct gcaccgtttt cccccattaa tgcatggata
    15901 gaatgtggcc ggacttttaa attaacgtta tcaagtgcct taacaccagg aaaggacttg
    15961 ttgataccgc tcatttccaa caagtattcc ccggaagacg gagtcgttga gctgaccata
    16021 taattttacc ttgttggcca tacaataagg gcgcagtaat agactgcgcc caatcagtct
    16081 tatttcttgc tgaattcagc caggttgtct ttatctacgc caacataagg tacgcggacc
    16141 actttgttgt cgattttcca gttggtgcca tcagccgcac ctttaccatc ggccaggttt
    16201 ttcgccagat caaaggtcgc tttcgcctgg ttgttagcat cgttcagtac ggtgcccgcc
    16261 agtgcaccgg atttcaccag cgccagcgct tctggcagag catcgacgcc aaacaccgga
    16321 atgctggact tgttgtgtgc tttcagtgct tctaccgcgc ccattgccat cgcatcgttg
    16381 ttggcgataa ccacttcgat tttgttggcg ttcgggccag acagccaggc gtccatctta
    16441 tctttcgcct gagcggtgtc ccacattgcg gtatctaact gtaactgttc agttttaatg
    16501 cctttgtcgt tcagctcttt aatcacgtaa gtggtacgtg cttctgcatc cggatggccc
    16561 ggttcacctt tcagcagtac gaattgaatc tgaccgtctt tattcagatc ccaaccctga
    16621 ttcgccgccc agtgtttagc aatcaaatcg ccctgaataa tgccggactc tttggagtca
    16681 gtgccaacgt agtaggcttt gtcgtagcta tccagcgcct tacgagacgg ctctttgtta
    16741 aagaaaacca ccggcacgtt ttgcccacgc gctttctcaa tcaccgtacc cgcagctgcc
    16801 gggtcaacca ggttgattgc cagtgccttc acccctttcg ccagcaatac gtcgatctga
    16861 tcgttctgct tggactggtc attctgagaa tcattcatca gcagctgaac atctggcgcg
    16921 gctttcgcat cttgctcaat agccttgcgc actacagaca taaagttatc gtcgtactta
    16981 tagattgtta caccaatgcg agtatcagca gcgtgtgcag cggcaccgaa taacatgctg
    17041 gccatcacag cagacagggt taacaccttc ttattcatgg tatctccggt ttttcttatg
    17101 cagggtagtg cttgagataa atgctcggcg gggcagtaga gttaatgaag tgttactgaa
    17161 cgccgaagct cactttttaa aattcgttct tccatgcccg gtaacgctcc agaaaacggc
    17221 tttaattgtt gttatgacgc tattacctcg gtgaaagtga ttattcactg ttacatacgg
    17281 gttacaacgt taaaacggtg caatcatagc tatcacattg ttaagatact gtgaaatcac
    17341 tcacagattg aaagcggtta catcgcctga tttgttgagt tagtgatcgc cgccgcattc
    17401 tggcgcgttg cgacagaatg gcgacgcact aacgtcggca taaaacagtg gctggcacga
    17461 ggatcaatat tgcctgctgc accctgcaag gccagttcgg tggctaattt agccattgaa
    17521 gcaatgggat aacgcacggt cgttaattgc gggtcggtgt aacgggcgat gggaatatca
    17581 tcgaaaccga tgattgagag atgtaacgga atcgcaatgc cattatcttt taatgctgtc
    17641 agtgcgccag cagccatatt gtcgttataa gcaaatacag cggtaagttg tagattgcgc
    17701 cccagcagtt caaccatcgc cgcctcaccg cccggcatgt ccggcgtacc agtgccaatc
    17761 cagctttccg gcggaataat atcctgctct ttcaacgcac tcatccagcc tgctttacgc
    17821 atggcgtcat cttcaatgcc gtggctggaa gaaagataac caatacgttg atgaccgtta
    17881 ttcagcaaca tgcgcgtcgc cattcgggca ccgctgagat tatccaggca aacgcaacga
    17941 tgggcgtacc ccggcacaac gcggttgatt aacaccatac cgggaatgtt atccataaat
    18001 tgcgccagtt catcgtcact caatgctttt gagtgaacaa tcaatgcatt acaacgctgg
    18061 cgaattaaca cctcaatggc gtgacgttct ttttccgctt catgatagct attgccgatt
    18121 agcacgtatt tctgatgctg ctgggcgacc agatccaccg cttttaccag cgcgccgaaa
    18181 aacgcatcag aaacgtccat caccaccacg ccaatggtgt cgctaacctg agttgccagc
    18241 gcctgggcat tggcgtttgg ccgataatcc agctcactca cggctttcat tactgcttca
    18301 cgcgtgtcgg cactgaccag cgtgctgtta ttgagcaccc gggaaaccgt tgccacagag
    18361 acgccagcct gacgcgctac atcacgaatg gtgatcatat tcactacctg ttatgagatt
    18421 gcagtaaatg actgcttgct ggcggctatt ttgtcagcac tgaatacagg acttcgtgaa
    18481 tcgagtcaca gcaatggaaa cggttacagc cgttttgtta acgaatgtga tccagatcgt
    18541 tatcttgatg ttttagatat tgccggtccg gcagcacgca aagttaactg acgccagagc
    18601 cattccaccg gcccctggcg gaagtaacgc agccagataa cagagaagag aatattcgcc
    18661 agccataccg gaataacaaa cgccagcaat cccagacggt caaactgcgt aaacaaaccg
    18721 aggtgataaa aaagcgtggt acaaatcagc gtttgcaata gatagttggt cagcgccatc
    18781 cgtccgacgc aggcgatcgc aagcaccagc ttaaagcggc tcaattgcgg ccagaaacca
    18841 taaaacagcg acgcatagcc aatcgcctga aacggtgcac tcagttcccg cggcatctga
    18901 agtaagaagg cgcaccagcg atatgcccag tccagccgcc attgcagggc gatggcagga
    18961 aggttaatga tcaccccaat cgccaccagc acaaaaccag tacgacgata gtgacgtaag
    19021 ctgaactgcc ctttcagcca gccactgcgc atcaatgcgg caccaatgag catcatccca
    19081 gccagttgcc agccatactg tgcgcccagt gccagtaaac tgttgccaac accatcggca
    19141 cgattactga tcgcttcaac gccgccgtga agcttccagt atttttcata caaaatagcc
    19201 gatgcatccg gcgtccaggc gcggctggtc tgggtatcgg aaatcagccc caataacagc
    19261 aaaacgccaa gccccaccag ataaagcatg acgccggtat taaacaggct ttttaccgat
    19321 ggcgcatcgc gcaccagccg ccagcagatt aagcccacca gcccgtaagc cagcagaata
    19381 tcgccgtccc agaacaataa accgtgaata aagcccagca agactaacag cgttaaccgc
    19441 gactggatcc agcgtctgcc acggggcagc aacatttgca ggccagcacc aaacagcagc
    19501 gcaaaaagcg tgaggaattt cacctggccg atgagatcga gaaatgccca ggtccatgca
    19561 tcctgcggcg taatagcgcc gtaccaggcg ggattaagat aagccgcctt tggtagccca
    19621 aaggcgctga tgtttaatag caggatcccc agaatggcga cgccgcgaac aaaatcgagc
    19681 gtgacgttgc gctccatggt tcctgccttt taatcagttg tgatgacgca cagcgcgcag
    19741 aaactcgtgg cgcgtattct gactggattt gaacaatcca ccaagagagg tcgttgtcgt
    19801 ggcactggtt gcatcgcgga tgccacgcgc cttcacgcag taatgcaccg cgtcgataga
    19861 gacagccacg ttattggtgc ccagcagcgt ttgtagcgca ataagaattt gctgcgtcag
    19921 acgttcctgc acctgcggac gctgggcaaa gaactgcacg atacggttaa tttttgacag
    19981 accgatcacc gaatctttcg ggatataagc caccgtcgct ttgccatcga tggtaacaaa
    20041 atggtgttca caggtactgg tcagagtgat atcgcgcacg gtgaccattt catcgacctt
    20101 cattttgttt tcaatgaggg tgattttcgg gaagttggcg taatccagac cggagaaaat
    20161 ttcatcgaca tacattttag cgatgcgatg cggcgtttcc atcaaactgt catcagccag
    20221 gtcgagattc agcagctgca tgatttcggt catatgacca gcaataaggc ttttgcgcgt
    20281 ttcgttatcc atttcatgca cgggcgggcg cagcggtgtt tccagtcctc gcgcaactaa
    20341 cgcttcatga accagggccg cttctttact gagtgatggc atttatgatt tctcctgcag
    20401 gtgtgacgcc tccgccctgc gtgggggcaa agttattaag ctgatttaca gcctgattat
    20461 tgtgcgtgag gcggcgcaca taatccagta ttcacagcga taattattgt aattgccgct
    20521 gcctttcatc agcagatgtt aaaacatcgt tatgcaaata cggaagtgaa agttactcac
    20581 agcacattga ataaacggta tgatgaagaa attgcaaaca acacaacaag gagccacgca
    20641 tggaaatgct cgaagagcac cgctgttttg aaggctggca gcaacgctgg cgacacgact
    20701 ccagtacctt aaactgcccg atgacgttca gtatctttct ccccccccct cgtgatcaca
    20761 ccccgccacc agtgctgtac tggctttccg gattaacctg caatgacgag aacttcacca
    20821 ccaaggcggg tgcccagcgc gtggcggcag aactggggat tgtactggtg atgccagaca
    20881 ccagcccgcg cggcgaaaag gttgccaacg acgatggcta cgatttaggc cagggcgcag
    20941 gcttttatct taatgccacg caaccgccgt gggcgacgca ttaccggatg tatgattatc
    21001 tgcgcgatga attaccggcg ctggttcagt cgcaatttaa tgtcagcgac cgctgcgcta
    21061 ttagcggtca ttcaatgggt ggtcacggtg cgctgattat ggcgctgaaa aacccgggca
    21121 aatacaccag cgtttccgcc tttgcgccaa ttgtgaatcc gtgcagcgtc ccgtggggaa
    21181 tcaaagcgtt tagccgctat ttaggtgagg acaaaaatgc atggctggaa tgggacagtt
    21241 gcgcgctgat gtatgccagt aacgcgcagg atgcgatccc gacgcttatc gatcagggcg
    21301 ataatgatca gtttcttgcc gatcagttgc aacccgcagt tctggcagaa gccgcgcgcc
    21361 agaaagcgtg gccgatgacg ctgcgtattc agccgggata tgatcacagt tactacttca
    21421 tcgcctcttt tatagaggat cacctgcgct tccatgcgca gtatttactg aagtgaaagt
    21481 ccgcccggtt cgccgggcat cttctcatca gaagcgataa tccactgcca taaagtaacg
    21541 acgtccgtct tcgttatagc tgtagtcgtc acgactgaga tccttgtcgc caaggttcag
    21601 cacgcctgcg cgcagtttga cgtctttagt cacctgccag gccgcgccgg tattccagat
    21661 ggtataaccg cccggtgttt tagccgtcgc gctgtcggct cgtttctgcc cggtatagtg
    21721 accagaaaca tagaatgacc agtcttccag cgccagcggt ttccagtcca gcgtaccgtt
    21781 ggcggtatgg aacggcagat cggatagcgg tttgttttcg ccgttgctga catcacgacc
    21841 atcgttgtag gtgtagttga gtgacagttt ccattcatcg ttgaacggaa ttttcagctc
    21901 ggtttccacg ccctgaatac gtgctttgtt aacgttgtag tagctaaata ccggcacacc
    21961 gttcgctgta aaaccaacaa agttttggta gcccggtgca gcattgacgt cagacgtacg
    22021 gctgatgctg atacgatctt tcacatcgtt acggaaaacg gtaacgctgg attcaacccc
    22081 ttccagccag ccttcttcac ccatgtagta cagccccagc tcccaacttt cgctggtttc
    22141 tggtttcaga tccgggctac ccacaatctt acatgcgcca cggcaggaat tgctcgtcca
    22201 gtcagggcta agttgcaaca gagaaggtgc tttaaatgcc gtcgcccagc cccctttcac
    22261 cgttacggtg tcggtggcgt tataaaccag gtaggcacgc ggactccagt gttcaccgta
    22321 ggtttcgtga tcgtccatac gcacgccggt cgtcagcgcc agcggctcga agatccgcca
    22381 ttcatcttcc acaaacagcg cgtactggct ggcagacgtt ttggagctgg ttcccccggt
    22441 caggttcacc gcatcgctaa gtttgtcgtg acgccattca ccgccaaccg tgagaaactg
    22501 attaatcgcc gtcagcggca acgtgtattt gccgtcgacc gtattgcttt cggaagttat
    22561 cgggctgctg ttgccagggt ttttgttctc gactttctca ccgtagtatt tcagttcgct
    22621 ggtgccgtaa tcccaacgcc cattatggct gacggagtag ttctggcgtt ccaggcggtt
    22681 tttgtccagc gagtcggaat cacgatcctg acggtcgaaa ccgtatccgg cagtaaaatc
    22741 gtgattttga ttcggtgtcc aggcaaattc gacattgccg tcgcggctgg agaatccttc
    22801 aatacgcggc gtttctccgg tatcggtggt cgttgagttt tgcgggtcat ccttttcacg
    22861 ttttgccagg ctgccgtaag ctttcattcc cagcacacca tcaattaatg gtccactggt
    22921 aaagaactga ccgttatagg tatcaccgcg atcgcgatgt tcctgaatgg tggtatcgac
    22981 ggtaacggta cccgaccatt tctgaccgat ttttttggtg atgatattca ctacaccgcc
    23041 gagcgcatcg gaaccgtaca gcgacgacat cgggccacgg accacttcaa tacgttcgat
    23101 ggaatcgacc gggatccagt tcagatcgaa atcattgtgg cggaagacgg cattgcggga
    23161 gttaacgcgt ttaccgtcga caagaatcag ggtgtagctg ctgtccagac cacgaatact
    23221 aacgccctta cggttatccc cttcgttcgt cagttgtacg ccaggcactt ctttgaggac
    23281 atccttcaga ttctgtaccg gttttcgctg caggtcttcc tgggtaatga cgctgatact
    23341 ggcgggtgca tctttaaggt tttgttcaac ggaagatgca gtgacaacca tcgtttcgcc
    23401 atcatcatcg accgctaaca caggccatgc acaagaaata gcggacaaac acagcccgac
    23461 ccgtacgaaa gggttcaacc taaacattcc atatctccat gaggtaacta cgaaaataaa
    23521 atgggttatc gctcacatct tcttcacgtc cccttgcgta cggcagcatc gcggtggact
    23581 tatttgtatg caggtgatca tcccagaaaa ccggaccagc ctctgatttg tcagcctttt
    23641 ttgattgcgg cgtaacgata atgcaaacga taacaattat caatccaaat gttaaatttt
    23701 atatccgcat gatttgtcag ggaaatggaa ttgaaaaagc cctctcggtt gagagggctt
    23761 agcaaggaag ggagaaactt atttcttatc gttctgcggg aacttcattt cgctgtagcg
    23821 tacgaagtgg gttcctttaa tcagcttgta gccgaaccaa ataatcagga acagcgggat
    23881 accaatatac gttgccgcta caccgcccca gtcaatggta tctttcagga atgcttcgta
    23941 gttctggccc aaagtgataa tcagacacag aatgaatgcg aagatcggac ccagtgggaa
    24001 gaaacctgaa cggtacggca gatcgttaat gtcgtgtccc tgcaatacgt aaccgcgacg
    24061 gaagcgatag tggctaatgg caatccccag ccaggcgata aaaccggtca tcccggaggt
    24121 gttcagcagc cacaggtata ccgtctggtt gccaaacatg gaggtcagga agcacagacc
    24181 ggcaatcacc gtcgtcgcat acagcgcatt acgcggcacg ccaccacgcg acagtttagc
    24241 gaaaatgcgc ggcgctttac cgtcacacgc cagggtgtac agcatacgag tagacgcata
    24301 cataccggag ttacccgctg acagcaccgc cgtcagaata actgcgttca tcaccgccgc
    24361 cgcagagagc agacccgcgt gctggaacac cagggtgaac ggactaacgc tgatgtcttt
    24421 aacatcgtta cgtagcaggc ttggatcggt gtacggaata atcaggctga taatcaggat
    24481 cgcgaacaca tagaacaaca ggattcgcca gaacacctga cgtaccgcgc gtggaatgtt
    24541 tttccccgga tcttcggact cgcctgcagc aataccgatc agctcggttc cctggaaaga
    24601 gaagccgaca atcatagcta cgccgatcat cgccgcaaaa ccaccagcaa acggcgcttc
    24661 gccgattgtc cagttgctcc agcccgcagg ctgcgcgcct ttgaagatac cgataatcat
    24721 cagcacgcca acgatgataa agacaataac tgtcgtgact ttgatcagtg agaaccagta
    24781 ttccgcttca ccaaagccgc gaacagagat gtagttcagc aggaagataa cgccgaggaa
    24841 caacgcactc cagatccagc ccggtgtatc cgggaaccac cagctcatga ccagctgagc
    24901 tgcaaccagg tcaacggcaa tagtcaccgc ccagttgtac cagtagttcc agcccagcgc
    24961 gaagccaaag ccctcttcaa catagttctg accgtaagtg gcaaacgaac cggaaaccgg
    25021 catatatgca gccagttcac cgagactggt catcaggaag taaaccatca ggccaatcag
    25081 catatacgag agcaatgccc cgcccggacc tgcctgagaa atcgttgcgc cagaggcaac
    25141 aaaaagacct gtaccgatgg aaccgccaat ggcaatcatc gtcaggtgac gcgcctttaa
    25201 ttcacggcgt aagcccggcg cttctgtggt tttagtttcg gaaaccatac aaaaatgcta
    25261 tccatcttaa aaatgaggcg cgattgtagc agacgattgg caatccttcc ggcagaaata
    25321 cacggttata agacaccttc atgatcgccc agggattata agtaaagcag ccaatctctt
    25381 ttctggcgaa tgcatgctga aggcgctgcg tcacgaaatc gacacagcac cagcatgttc
    25441 ttgtacagca acttaacgcg ggacattcgc gggatcgcaa tagtccagaa agcgccgtag
    25501 cgcgttggaa aggtgttttt gccgatgatg tatacgccac aacgtacgca tcaggcgcgg
    25561 cagagggacc gcaacttcac ttaatgtgcc tgcctgcaat tggtcttcaa tcacccgtcg
    25621 cgacaggcaa ctaattccca acccatgacg caccgcatgt ttgattgcct cggagttacc
    25681 taattccatc gccatctcaa acttcggtaa atgtgacagc aacagataat cgacaatctc
    25741 ccgcgtgccg gagccgcgtt cacgcaggat ccacggcgca gcggccagct gttctaaggt
    25801 gaccggacca cgggccaacg gcgaagtcgg cgcggcgaaa accaccagct cgtcttccag
    25861 ccatggttca gaaatgattt cagtgctgtg gcacggtcct tcaataaagc caatatcaac
    25921 gcggaaatcc agcaccgctt gcatcacgtc ctggctgttc ccaacgctaa gttcaatcgg
    25981 caactgcgga taatcatggc gataacgggc gataactgca ggcagaatgt agttaccgat
    26041 ggtactactg gcatagatac gaatcgcgcc gttgtcttcg cgaaacagtt gttcgatttc
    26101 aaccgcctgt tcaagcagtg ccaacgcacg cggatagagc agccgcccgt gttcattaac
    26161 aaccagtctt ttccccacgc gatcaaacag ttgcacgcca agctgccctt ccaggtcggt
    26221 caaggctgcg ctcactgctg attgcgacaa cgccagcatc accgacgcct gggtggttga
    26281 tccacttttc aatacttctg caaaaacttc caactgccgg agggtgatgt gcatagtcgc
    26341 ttaccactta taaagattaa ttataaatat ataatcaatt ttatttttaa accagttagt
    26401 cgtaacctta tgcccggtta aaggagaggg ttatgacgaa tatcacctta cagaaacaac
    26461 atcgtacact gtggcatttt attccggggt tagccctgag tgcagttatc accggggtcg
    26521 ccctgtgggg tggttccatt cccgcggttg cgggtgccgg gtttagtgcc ctcaccctcg
    26581 caatcttgtt ggggatggtt ttaggcaaca ccatctatcc gcacatctgg aaaagctgtg
    26641 acggtggtgt gctgtttgcc aagcaatatt tattacgtct gggtatcatt ctttatggct
    26701 tccgtctgac gttctcgcaa attgccgatg tcggtatcag tgggatcatc attgacgtgt
    26761 tgacgctgtc cagtaccttc ctgctggctt gcttcctggg gcagaaagtg tttggtctgg
    26821 ataagcacac cagctggttg atcggtgccg gtagcagtat ctgtggtgct gccgcggtac
    26881 tggcgactga gccggtagtg aaagcggaag ccagtaaagt aaccgtggct gttgcaaccg
    26941 ttgttatctt cgggaccgtc gcgattttcc tctacccggc gatatatccg ctgatgtctc
    27001 aatggtttag tccggaaact ttcggtatct acattggttc tactgtgcac gaagtggcgc
    27061 aggtggtggc ggcaggtcat gccatcagcc cggatgcgga aaacgcagca gttatttcca
    27121 aaatgctgcg cgtgatgatg ctggctccgt tcctcatcct gctggcggcg cgtgttaaac
    27181 agctgtctgg ggcgaacagc ggcgagaaaa gcaaaatcac tattccgtgg tttgccatct
    27241 tgttcatcgt agttgccatc tttaactcgt tccacctgtt accgcagagc gtggtgaaca
    27301 tgctggtaac gctggatacc ttcctgctgg caatggcgat ggcggcgctg ggtctgacca
    27361 ctcacgtcag cgcgctgaaa aaagccgggg cgaaacctct gctgatggca ctggtactgt
    27421 ttgcctggct gattgttggt ggtggtgcga ttaactatgt gattcaaagc gtcatcgcat
    27481 aaaccactac atcttgctcc tgttaacccg ctatcattac cgttttcctc cagcgggttt
    27541 aacaggagtc ctcgcatgaa atacattgga gcgcacgtta gtgctgctgg cggtctggca
    27601 aatgccgcaa ttcgcgccgc cgaaatcgac gcaaccgcgt ttgccttgtt caccaaaaac
    27661 caacgtcagt ggcgtgccgc accgctcacg acgcaaacca tcgatgaatt caaagccgcc
    27721 tgtgaaaaat atcactacac atcggcgcaa attcttcccc acgacagcta tctgattaac
    27781 ctcggacatc cggtcactga agctctggaa aaatcgcgcg atgcctttat agatgaaatg
    27841 cagcgttgcg aacagctggg gctttctttg ctcaacttcc accctggcag ccatctgatg
    27901 cagatttcag aagaggattg ccttgcgcgt attgccgaat ccatcaacat tgcgctggat
    27961 aaaactcaag gtgtgaccgc ggtgattgaa aacaccgccg gtcagggcag taacttaggg
    28021 tttaaattcg aacatctcgc ggcgattatc gacggcgtgg aagataaatc ccgtgttggc
    28081 gtctgcattg atacctgcca tgctttcgct gccgggtatg atttgcgtac tccagccgaa
    28141 tgcgagaaaa cgttcgcgga ttttgcccgt attgtcggct ttaagtatct gcgcgggatg
    28201 caccttaacg atgcgaaaag cacctttggc agccgcgttg accgccatca tagcctcggt
    28261 gaaggcaata tcggtcatga tgcgttccgc tggatcatgc aggacgaccg cttcgacggc
    28321 attccgctga tccttgaaac catcaacccg gatatctggg cagaagagat cgcctggctg
    28381 aaagcgcaac aaactgaaaa agcggtagcc tgaagatgaa taaccgggaa aaggagatcc
    28441 ttgcaatttt acggcgtaac ccgctgattc agcagaacga aattgcggac atgctgcaaa
    28501 tcagtcgttc gcgcgttgcg gcgcatatta tggatttaat gcgcaaaggc cggattaaag
    28561 gcaaaggtta cattctcacc gagcaggaat actgcgtagt ggtggggaca atcaatatgg
    28621 atattcgcgg gatggcggat atccgttacc cgcaatcggc ttctcatccc ggtacaattc
    28681 attgctcagc gggcggcgtg ggacgcaaca tcgcccacaa tctggcgctg ttaggccgcg
    28741 acgtccattt gctttcagtg attggcgatg acttttatgg cgaaatgctc ctggaagaaa
    28801 cgcgccgtgc cggcgtgaat gtctccggct gcgttcgttt gcatggtcaa agcacatcga
    28861 cgtatctggc aattgccaat cgagacgatc aaaccgtgct ggcgattaac gatacccatc
    28921 tgctggaaca gttgacaccg caactactga acgggtcgcg cgatttactt cgtcatgcgg
    28981 gcgtggtact ggctgattgc aacctgacag ccgaggcgct ggaatgggtc tttaccctcg
    29041 ctgatgagat cccggtgttt gtcgataccg tttcagaatt caaagcgggc aaaatcaaac
    29101 actggctggc gcatattcac accctgaaac ccactttacc ggagctggaa attttatggg
    29161 gacaggcgat caccagcgat gctgaccgta atgctgcagt gaatgcgttg catcagcaag
    29221 gtgttcagca actgtttgtt tatttgcccg atgagtcagt ttattgcagc gaaaaggatg
    29281 gagaacaatt tttgctgacc gcaccagcgc atacgacagt agacagtttt ggtgctgacg
    29341 atggttttat ggcgggcctg gtatatagct ttctggaggg aaacaatttc cgtgacagcg
    29401 cccgttttgc gatggcctgc gcggcaattt cacgcgccag cggcagctta aacaacccta
    29461 ccctgtctgc cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc
    29521 gacgcttgac cgcatcttat ccggctcata aatcgcacac tacgctaaac caataaagaa
    29581 cccggcaatg gtggcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc
    29641 aagctgggcg atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac
    29701 cccgatagaa ccaaagttag cgaaaccgca caacgcgaag gaaataatcg ccacggtttt
    29761 tgcatcgaga gtgccagctg tttgcagata gggtgagaaa ttgagataag cgacaaattc
    29821 atttattgcc agtttctgtc caatcaaact cccggcaaga ttcgcatcag tccagtcaac
    29881 acccattacc cacgccagtg gggccagcag gtaacctaaa atggactcca gcgaggcatg
    29941 ttcaaaacca aaccagccgc caacgccgcc gataataccg ttaattaacg caatgatggc
    30001 aacaaatgcc atcaccactg ttgccacacc cgcagcgatt ttcagcccgg tcaccgcccc
    30061 tgtcgcggcg gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa
    30121 ggaaacctgt gaagattccg tagccgggct tagcatgcgg gcaaacaaga tcccgccggg
    30181 gatcgccatt aacgatgccg ccagcaaata ttccacaggc acgcccagtg cggcgtaacc
    30241 aatcatcgtc gaaccagcaa ttgaggccat gccactacaa atcgctgtaa ataactcatt
    30301 gcgattcagc cgatcgataa agggtttgac gattgccgga atttcgtttt gcccgaggaa
    30361 aatggtagtg accgcgacga atgactcgat cttgctgata tttaatgctt tctggaatat
    30421 accgccgaga atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag
    30481 cgcggtgacg aagataattg ccggtaacac cctgaaacca aagataaatc ctgcgccatc
    30541 aaataaggtg tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacccgcgtc
    30601 gctgtacgcc atcactttat gcacgccaaa agcgactttt tcagcgaccc aacgccctgg
    30661 cggtaaccaa agcataatgc cgccaatcac gacctgtaac actaacgccg cgccaacggt
    30721 acgcaggctg atcttcttct tgtttactga cagtaaaaac gcaatcgtca gcaataccac
    30781 cattcccaga acacttctca tgacatccat agttattatc tcttcatgtc atattgggcg
    30841 acggctttct gcccctcaga aatagccggg cctgcatctt gcgatactga cccggcttgt
    30901 tatcaacact taatgggttt tgatgtagcc gcgcacgcac tcttcgacta agccccagaa
    30961 ccagtcggta tcaatagtga tgccgacttt ggtattagcg ggcttgccaa gaacgcccag
    31021 ctcgtcgcag acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat
    31081 ctcctgggtt ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac
    31141 cgggccgcca gccaggccgt agttttcgaa ctgggttttg agagtgaagt tcatgatgtc
    31201 gctgaacagc tctccggcag ggccgcctgc cctttccatc cgagcaatta cgtccggggt
    31261 gcaaacggtc tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa
    31321 caccacgcgt gcggcttccg ggtcagcaaa gatgttgaac tcggcagatg gcgtgaagtt
    31381 gcctgtaccg taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg
    31441 ttgcatacgc attgccaccg cgatatttga caacggacca accggcacca gagtgatatc
    31501 gccatcgctt gccatcaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg
    31561 gcgggtcagc ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc
    31621 aacgatttgt tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc
    31681 cagtttctgg caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc
    31741 aacaatggtg atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat
    31801 agcatcatca tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt
    31861 cctctgtttc cagttgcgtt attttttcta cagcaaagaa atttcgatcg ggccgatgat
    31921 tgaatcttaa caacagcgta cgtatgctaa atatgagaaa tctcatagcg gataaacatc
    31981 gtgaaagaaa tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag
    32041 gattgctttt caacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac
    32101 tacattgtgc aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc
    32161 aggctttacg ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc
    32221 tgttttattg gtgagattga gttaatcgat aaagaccatg aaccgcgtgc ggtacaggct
    32281 attgaagagt gttggtgcct tgcgctccct atgaaacatt accgtccgct gttattaaac
    32341 gacaccctat ttttacgaaa actctgcgtt actttaagcc ataaaaatta tcgcaatatt
    32401 gtttctttaa ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta
    32461 ctctcacaag aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc
    32521 gtttcttatc gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt
    32581 aaaagcaaga aaggatatct aattaaaaac agaaagcagt tgtcagggct ggcgctggag
    32641 atggacccgg agaataaatt ctccgggatg atgcagtaaa aattatttgc aatagcgcga
    32701 ttgccggatg caacgcttaa taagcgagta catccggcaa tgaaactcaa gctaaaccaa
    32761 taaagaaccc ggcaatggtc gcgctcatca ggttggaaag cgttgctgct gccagcgccc
    32821 gtaaaccaag ctgggcgatt tccggagcac gttttggcga aatagccgaa aatgccccaa
    32881 cgacaacacc gatagaacca aagttagcaa aaccacaaag cgcaaaggag ataatcgcaa
    32941 tggttttcac ttccagcgtg ccgcccgttt gcaggtaggg ggagaaactc aggtaagcga
    33001 cgaattcgtt aatcgccagt ttctgcccaa tcaggctacc cgcaaggttg gcatcactcc
    33061 agtccacacc catgatccac gccagcggtg ccagcacata gccaaaaata ctttccagag
    33121 aagcattggt aaaaccaaac cagccgccaa ttccgccgat aatgccgttg atcagcgcaa
    33181 taattgcgac aaacgccatt accaccgtcg ccacaccggc ggcgattttt agcccggtca
    33241 tcgcaccgct cgccgccgct tcgataaagc tttttggcgg cgtttcactg aacgacagat
    33301 tttcaaatgt gacctgcgaa ggctcggttg ccggactaag aatacgtgca aacaaaatac
    33361 cgccggggat cgccatcagc gatgccgcca acaggtagtc aattggtacg cccattccgg
    33421 cataaccaat catcatcgaa cccgcaatgg acgccatccc gctacaaata gccgtaaaca
    33481 actcgtttcg attcatgcga tcgataaacg gtttaacgat cgccgggatc tcattttgcc
    33541 cgaggaaaat agtggtgact gcgacaaaag attcgatttt gctgatgttg agggctttct
    33601 ggaaaatgct gccaaggatg cgaattagca gccccatcac gccaatgtag tacagcagac
    33661 taatgagcgc agtgacgaag ataatcgccg ggagtacgcg aaaggcgaag ataaaacccg
    33721 caccgtcaaa gaggacatcc attttcggcc cgaccagcga accaaaaatg aaggcgctac
    33781 cggcatcact gtaagacatc actttatgaa cgcctaatgc cgcctgttct actgcccatt
    33841 ttcccggcgg aaaatagagc ataatgccac caatagcgat ttgcagcagc agtgcggctc
    33901 caaccgtgcg caaactgatg ctctttttat ttaccgacaa cagaaatgct attgccagta
    33961 acaccaccat ccccacaaca cttctcatta tatccataat gattttccct tcatgccagt
    34021 aaacccggcg tcagcgccag gttttggtat gtttgatgag tacgggcgac ggctttctgc
    34081 ccgtcagaaa ttaacccgcg agacgctgat attctttggc aatttcgctc gccagaatgg
    34141 cgttgttgaa caccagctgg atgttggatt tcaggctgtc accgccggtc agctcagcaa
    34201 cgcgcgccag caggaatggc gtactttctt taccaataac accctgcgct tcagcttcag
    34261 ctaccgcctg atcgatcgcc gcattgatag tgtgttccgg catagcaaac tgttccggga
    34321 tcgggttcgc taccacgagg ccaccgttca gcccgctttg ccatttcacc gccattgcac
    34381 gggcaatttc gctggcgctg tcgagacgaa tgctgacgtc aaacgggctg gtgcggcaga
    34441 aaaacgcagg cagcgctttt gtctgatagc caattaacgg cacaccgaag gtttctaaat
    34501 actcagtggt taatcctaaa tcgagaatag atttcgcccc ggcacaaaca acggtgacat
    34561 tagtatttgc cagttcttgc aaatcggcag aaatatcgaa ggtatgttcc gccccgcgat
    34621 gcacaccacc aattcccccg gtggcaaata ctttaattcc ggcaagcgcc gcaataatca
    34681 tcgttgaagc cacagtggtt gcgccatttt ttccggcggc aacaacaaaa ggtaaatcgc
    34741 gacgactaac tttggtcacg ttatgccctt cacgacccag taattcaatt tcttctttgc
    34801 ttaaacccac tttcatcacg ccgccaataa tggcgatagt ggcaggtaca gcgccctgtt
    34861 tacgaatagt ttcttcaact tcaattgcgg tctgggcatt ttgtgggaac ggcatcccgt
    34921 gagaaataat ggtcgattcc agcgccacaa ccggtttttt gttttttaaa gcgtcctgca
    34981 cttccgggga aatttgtaat aattcagggg aaatttttaa ttcagacatt ctgcgttctc
    35041 cactaacgat ataacgttgg caatcgataa atcggggtta ttggtgtatt cacaggagag
    35101 cgccattgac gaacatccct gtgcgaaacg aacagattcg gcaaacggca ttccgtctac
    35161 ccaacacgaa gcaagtcccg ccatcatggc atcgcccgct ccggtaacat taataacatt
    35221 ggttttgatc ggcgcagacc agccactttc accgctgata tcgctgtaat aaacgccatc
    35281 gccgcccatg ctcaatacca gtcggttcag gccatgttga tggaaccagg cagcaacttt
    35341 tgccacatct tcacgccctg acagcgcaat cccactcagg gtttccgctt caaggcggtt
    35401 tggcttgaga gtgtggatca gatttagacg gtcgcgcact ttgacacatt tccacgcgga
    35461 aacaggatcg acaaatacgg gtacgttggc ggcattatcc agaatccatg ccagtgcctc
    35521 ttcactgata ttacagtccg cgacaatgac ctttgcactc tgaataaatt cacggtgctg
    35581 tgcaagatat tcagctgtaa tagcgttgct aatattcatg tcatttatag caaccagcat
    35641 ttcaccggta ttatcgagta atgataaata actcgacgta ttttctcccg gcacaatcag
    35701 gcatttatcg acataaacgc cagattgatt ggtttgcgtt agcagcgatt gaccataaaa
    35761 atcactgcct acggcgctca gtagccaggc tttgttaccc agcaacgcca ggttttgtgc
    35821 aatattgcgc cctactccac caggcgtaaa ttttatttta cctggatttg aatccgcata
    35881 atttaatgat tcatgtgaat atccggcgac atcaatattc gccgagccta taattacgac
    35941 ataatccttt tcgcgcatag cacgtcctct ggtaaaaaat taccaaccct gtcaatccac
    36001 aagaatatag atactgcaaa gacggtttaa acatgtgttc atatttaaac atgtgctcat
    36061 agtatgcatg ttttacgtaa agtaaattac cgccagagag gcaaaatggg cagatttgag
    36121 caggctcaca aaatgcgatt aaatgcctga attacaaaag cgaggaattt gtgacgagat
    36181 gcacagaaca ggaacactaa aaaagggcag aaaagaatct gcccttgagg aaataacctt
    36241 agtatttgat gaacgtttca ggtttctact gaaagataaa accatttaaa ttcataaaat
    36301 tacattcaca aaccggtcat tctctttgcg ctcatccaat gatacagggc agtaaatttc
    36361 tgccctgtaa cacacctttt attacgcggc tttcgctact gcgtccactt ccggacgttt
    36421 caggaaggca taggccaaac tcgccaccag cgtaccggca ataattgcta ccaggtaacc
    36481 cagtaccggc gtaatagcgc cagggatcag cagaacaaac agaccaccgt gcggtgccat
    36541 cagtttcgca ccaatcgcca ttgagattgc gccagtcagc gccccaccaa cgatacaaca
    36601 cggcagcaca cgcatcggat cacgagcagc aaacggaatt gcaccttccg aaataaagca
    36661 cagaccaaga accagagcgg ctttgccacc ttcctgctgc gctttgtcga atttgcgacg
    36721 cgccaccatt gttgccagac ccattgccag cggtggcacc atacctgccg ccataatcgc
    36781 cgccatcggg ccataggttt gagtactcag cagacccaca ccgaatgcgt acgctgcttt
    36841 atttaccgga ccgcccatgt cagtacacat catgccaccg aggatcgccc ccagcagaac
    36901 cgcattcgca gtccccatgg tctgcagcca gtgagtcaac ccttcgagaa tgccagcaac
    36961 cggtttaccg atcaggtaga tcatcgccag accgaccacc agactggaaa ttagcggaat
    37021 gatcaggatc ggtttcagcg cctccatact ctgtggcagt ttcagttgcg tactgattaa
    37081 cttcgcaatg taaccagcca ggaagcccgc aataataccg ccaatgaagc cagaaccggt
    37141 gctgaccgcc agcataccgc caatcagacc cggcgtgaga cccggacgat cggcaatgga
    37201 gaaggcaatg taacctgcca gtaccggcac catcagcgca aaggctgaac caccaccaat
    37261 ctgcatcagc gccgctgcca acgtacccgg ctctttaaac gcttcgatac caaaagcaaa
    37321 agaaagcgcg atacacagac cacctgcaac gaccatcggc agcatgtaag agacgcccgt
    37381 cagcaagtga cggtatgcgc ctgcactctc tttcttacct tcagaggtcg ccgtttgagc
    37441 tttgcccgcc ggttcatacg gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt
    37501 tttcttcagc gccagaccgg tagaggtacg atacatcggt ttaccagcaa atttcgccag
    37561 atccacttcg atatctgccg ccacaatcac cagatccgct gcggctactt cttcgggagt
    37621 gattgcatta cccgcaccaa cagaaccacg ggtttcaact ttcacccacc agccacgttt
    37681 tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg tgtgctacgc cagtcgggca
    37741 agcagtcacc gcaactacgc gtttcggacc gctggcggca accggtgctg tcgcagcgac
    37801 cggcgcagtg taaggtttcg catggccttt ggcttcactc aggaacagct cagggtgcgc
    37861 aactgcccgg gaaatatcgc ccagccagac atttttaccg ttcagcgcgc tgtcattcgg
    37921 gatggaatca ccgagaacaa tcgccatttc tgcgtcattc ggattgtcga tgatttccag
    37981 ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc
    38041 gagattagcg tcaataatca gcagcgtttt cattatgcct ctcctgctgt cagttaaaag
    38101 gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta atacccacat
    38161 tgctttgact taccgccagg gctgcaacag cagtcgccag acgcagtgtg tgttcactgg
    38221 attcacgcat tagcaagcca taaatcaggc caccaaccat agaatcccct gcgccaacgg
    38281 tgcttacgac atcgactgac ggtggtttgg cgatccattc gccggaggca ttaacccaaa
    38341 gcgcgccttc ggcacccagt gaaataacga catgcgcaat accttgttca cgcagcgcat
    38401 gcgcagcttc aatcacatct ttcatttcag gcagtttacg gcctgcccag atttccagct
    38461 cgcggcggtt aggtttcacc agccacggtg ccgctttcaa acctgctact aacgcttcac
    38521 ggctactatc aaagataatg caagggcact gactacgcag gcgagtcatc cagtcggtga
    38581 acgcttccgg gctgacgcct gacggtaagc ttccgctgac acagaccata tcgaactgac
    38641 cgagccagct cagagaatca gtcacaaagc gttcccagtc ggcgggagtg acttcaaaac
    38701 ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt cagcttaacg ttaattcggg
    38761 tgcgcccctg tacaacctgg aaacggttgg caatgcccag ctcgctgaac agttgctgaa
    38821 aaccatcctg attgtcttta ccgaggaagc cgccaacggt gacatcaatt cccagatctt
    38881 ttaatacttt ggccacgttg atgcctttac ccgccgcatg cagaccggtg gttttcacca
    38941 ggttcacttc gccgcgttca atttccgggc agaaaccaac aaggtcataa gccggattaa
    39001 gggtgatagt agcaacacgt ctgctcatta tgcgccctcc ccaagaccag cagcgatagc
    39061 gtcgccgatt gctttcagcg cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg
    39121 atgacctttc ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt ttgccggttt
    39181 gccggtgcca tcaaggtttg tcacggtaat atcactgtta aattgtttaa tggtgttgac
    39241 cagcatggta cctggacgag catgcaggcc gtgttcattg cgcaccacaa actccgcgct
    39301 taacacgtcg tcggtcggcg catcatcgct ggtcagcagc gccagcaacg ttgccgcatc
    39361 cgctttcagc aagcggtcag ctttattgtc gagcaacaaa tcagcgagac gcttaagaac
    39421 cgcaatgggc tgatcgtcat tcatcgccac actcaccagc atggctgccg tttcgccgtc
    39481 cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg ctacgcaaat tgccttcggc
    39541 gctatcgctc agccagatgc cctgtccgag gttcagcggt tgttcattga tggctttggt
    39601 gacgaaagtg gcgtcaactg cccccgcttc tttcagacgc gcagcgttca gcgcctgaag
    39661 agtcagcaga tcgctggcga cgatatccag cgtcagcatt tcgttgtcga gcttcagctg
    39721 ctcactctgc ttttcgccca tcagcaatgc gcgaagttct tctgctgttg ttgctgactt
    39781 cagttgttca gcaacggaat catcgctcag gacgtgggtc agctggcgta gcaggcccag
    39841 atgttcatcc gagctggcag caataccgat tgccacgtac gctacctgac cgtcacccca
    39901 ggtgacgcct tccgggaact gaaatacctg aacgccggtt ttcagcacct gatcgcgggt
    39961 gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg aacgttgagg tttgctgttc
    40021 gcgcgccagc atgccattga cgtagccttc tgctacatta ccggcctgca ccagcgccgc
    40081 agcgacctgg cgaatcgcct cttctttgtc tccggccttt tcgcccggat ggatgtcctg
    40141 tacggataac tggaacatag ttctcctctc ttgctgaatt gaaacgattc agcctctatg
    40201 agaaaaaaag cgccaaccta gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag
    40261 aaagcataat acttctgttt cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt
    40321 gatgtgctgc acattttttt gtcatttttc ctaattgctg atgggaaaat ccgttatcag
    40381 caatttcatt tcagcagcgt cagcagccaa tcagcaacag tgaaatggca ttttgattat
    40441 tcgtggcgct tttgatttcg ctgtctgttt attttctgac aagcagcgta aactccgcgt
    40501 cttcctcttc cagtgatcga ccagcatgca taactccccc gcagtctcca gcgcgaaatc
    40561 gtttgacctg acctcgacgg cgtttttaat cgttgccttt ctcaccggta ttgcgggcgc
    40621 tctgcaaacc ccgacactca gtatttttct taccgatgaa gtacatgccc gtccggcgat
    40681 ggtgggattc ttctttaccg gcagcgctgt cattgggatt ctggtgagtc agtttctcgc
    40741 cgggcgctct gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg
    40801 cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac tttgttttgc tattcgttgg
    40861 cgtctttctt agcagctttg gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga
    40921 acatgccgac aaaaccggac gtgaggcggt gatgttcagc tcttttttac gcgctcaggt
    40981 ttcactggca tgggtcattg gcccaccgct ggcttatgcc ttagcgatgg gtttcagctt
    41041 tacggtaatg tatctgagcg cagcggtagc atttattgtt tgcggcgtga tggtgtggct
    41101 gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc ggcacggttg aagcgccgcg
    41161 ccgtaaccgt cgcgatacgc tgctgctgtt tgtcatttgt acattgatgt ggggctcgaa
    41221 cagtctgtac atcatcaaca tgccgctatt tataatcaac gaactgcatc ttcccgagaa
    41281 actggcaggc gtaatgatgg ggaccgccgc cgggctggaa atcccgacca tgttgattgc
    41341 cggatatttt gccaaacgtc tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg
    41401 cgtctgtttt tacgcaggaa tgctgatggc gcattcacct gccattctgt tgggcttgca
    41461 gctgctaaat gctattttta ttggcattct gggcggcatc gggatgctct attttcagga
    41521 tttgatgccc ggtcaggcag gttcagccac cacgctctat accaacacgt cgcgcgtggg
    41581 ctggatcatc gcaggatctg tagcgggcat cgtcgccgag atctggaatt atcacgctgt
    41641 gttctggttt gcgatggtga tgattatcgc cactctgttt tgcttactgc ggattaaaga
    41701 tgtttaaggc gcggtcagca tttccagatc gataagccag gtcattgcct gctgacgcga
    41761 gttaccgcac atttcagcgc tggcttgtaa gcctgcacag actttcggcc gcagtggcga
    41821 ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg cagggcgtat tggcgggctt
    41881 gccatctggc ataccgggaa tagggctgga aattgaaggg gcagtgcaac acgccccgca
    41941 acccggacgg cattccatac gttgttctct taaggttaac caccggattg atgagcgcgc
    42001 acagtaccac cttttttgca ccagcaaaag tgcgaatacc acttgcccga aaggcccgtc
    42061 gcgagtactt tgtcgcgata tttttgacat tttcgactac aggaattttt cgatgccaag
    42121 agcgaacgaa attaaaaaag gtatggtact gaattacaac ggcaaactgc tgctggttaa
    42181 ggacattgat attcagtcgc ccactgcccg cggtgccgct acgctgtaca aaatgcgttt
    42241 ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc aaaggagatg atatcgttga
    42301 caccgtgacg ctgacccgcc gttacgttga cttctcctat gtcgatggca acgaatatgt
    42361 ctttatggat aaagaagact ataccccgta taccttcacc aaagatcaga ttgaagaaga
    42421 gttgctgttt atgccagagg gcggcatgcc ggacatgcag gtgctgacct gggacggtca
    42481 actgctggcg cttgagctgc cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg
    42541 catcaaaggt gcatctgcca gcgcccgtaa caaaccggcg acattgagca ctggtctggt
    42601 gattcaggta ccagaatact taagcccggg cgaaaaaatt cgtatccata tcgaagaacg
    42661 ccgttatatg gggcgtgctg actaacttca gccgcatgca gaaaagggat agctcaggct
    42721 gtcccttttt taatttatta taccaatctt ctattttgcg cttcctgaac accttacgcc
    42781 cgcaattttc actcgttgat tgatcgccct cacacttcat cgcattaaca atccagacca
    42841 atttcaattg ctgtcatata actttacact gtcattgtta attaatcgtt actaagacgt
    42901 gactcctatg aatacaatcg cctccgttac gctcccgcat catgtacacg ctccacgcta
    42961 cgatcgccag cagttgcaat cacgtatcgt tcattttggc tttggagcct ttcaccgcgc
    43021 acatcaggcg ttactgaccg atcgtgtgct gaatgcccag agcggcgact gggggatctg
    43081 tgaaatcagc ttgttcagcg gtgatcaact gatgagccag ctccgcgcac agaaccattt
    43141 atacaccgtg ctggagaaag gggcggacgg caatcaggcg ataattgtcg gtgccgttca
    43201 cgaatgcctg aatgcaaaac tggattcctt agcggcaatt attgagaaat tttgcgagcc
    43261 acaggtggca attgtttccc tgacgattac cgaaaaaggc tattgtattg acccggccac
    43321 cggtgcactc gacaccagca atccgcggat tattcacgat ctacaaaccc ctgaagaacc
    43381 tcactccgca ccgggtattc tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac
    43441 accgtttacc gtgctctcct gcgacaatat tcccgacaat ggccatgtgg tgaaaaacgc
    43501 ggtgctggga atggcagaaa aacgttcgcc agaactcgcc gggtggataa aagaacacgt
    43561 cagttttccg ggaaccatgg tcgaccgcat tgttccggct gcaaccgacg aatcactggc
    43621 agaaatcagc cagcatctgg gggtaaatga tccctgcgcg attagctgcg aaccgtttat
    43681 ccagtgggtg gtggaagata acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt
    43741 acaaatggtg aatgatgtcc tgccatggga agagatgaaa ctgcgtatgc ttaatggcag
    43801 ccactctttt ctcgcttatc tgggttacct ctcaggattc gcccatatca gtgattgcat
    43861 gcaggatcgc gcatttcgcc atgctgccag aacattaatg ctggatgagc aagcgccgac
    43921 actgcggatt aaagatgtcg atttaacaca atatgcggat aagttaattg cacgttttgc
    43981 taatccggcg ctgaaacata agacctggca aatcgcgatg gatggcagcc agaaattacc
    44041 gcaacgcatg ctggcaggta ttcgcataca tctggggcgc gaaacggact ggtcgttgct
    44101 ggcattaggc gttgcaggct ggatgcgtta cgtcagcggc gttgatgatg ccggaaatgc
    44161 cattgatgtt cgcgatccgc ttagcgataa aattcgcgat cttgttgcgg gcagcagcag
    44221 tgagcaacgc gtcaccgccc tgctttccct gcgtgaagtt ttcggtgatg atctgccaga
    44281 taacccgcat tttgtgcagg ccatcgaaca agcctggcaa caaatcgcac aattcggcgc
    44341 gcatcaggcg ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa
    44401 gctcgccttc gtccgctctc cccttctctt ttctgccgtt tttagccagg attaacgctc
    44461 agttaactta ccagagtatt gcggagccgt tgtgaccagg accaacctca tcaccggttt
    44521 tctcggcagc gggaaaacca cgtcgattct tcatctgtta gcccataaag atcccaacga
    44581 aaaatgggcg gtactggtta atgaatttgg ggaagtcgga attgatggtg ctttgctcgc
    44641 cgatagcggc gcactgctga aagagatccc cggcggctgc atgtgctgcg ttaatggttt
    44701 acccatgcag gtagggttga ataccttact gcgtcaggga aaaccagacc gcttgttgat
    44761 agagccgacc gggctgggcc atccgaaaca gatcctcgat ctcttaaccg caccagtcta
    44821 tgaaccgtgg atagatctac gcgccacgtt gtgcattctc gatccacgcc tgctgctgga
    44881 cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg gctgccgcag acatcattgt
    44941 cgccaataaa tccgaccgtg cgacacctga aagcgagcaa gcgctacagc gctggtggca
    45001 gcaaaatggt ggcgatcggc aattaattca cagtgagcat gggaaagttg acggtcatct
    45061 tctggatttg ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc attctcatca
    45121 gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca gagcatcaac gctggcgtcg
    45181 cagtctgaac agtggacaag gacatcaggc ctgcggctgg atattcgacg ctgatacggt
    45241 attcgacacc attggcattc tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg
    45301 cgtgctgcgt attcccgaag ggctggtgcg aatcaaccgt cagggcgatg acctgcacat
    45361 tgaaacgcaa aacgttgcgc caccggacag ccgtattgag ctgatttcca gcagcgaagc
    45421 tgactggaat ggcttgcaga gcgcgctgtt gaagcttcgt ttagcgacta ccgcgtaagg
    45481 ttgcctgcgt ttttcagtaa gataattaga gaaaatatga ttaaaaattt gccgcaaata
    45541 gtgttgttga atattgtcgg cctagcgctg tttctttcct ggtatattcc cgttaatcat
    45601 ggattctggt tgccgattga tgcggatatt ttttatttct ttaatcagaa actggtcgaa
    45661 agtaaggcct ttttgtggct ggttgcattg accaacaatc gcgctttcga cggttgttca
    45721 ctgctggcga tgggaatgtt gatgctgagt ttctggctga aagaaaacgc cccaggcaga
    45781 cgacgtatcg tgattattgg tctggtcatg ctattaactg cagtggtatt aaatcagctg
    45841 ggtcaggcat taattcctgt aaaacgggcc agcccaacat tgacttttac cgatattaac
    45901 cgcgtcagcg aactgctctc tgtccccacg aaagatgcct cacgagatag ctttcccggc
    45961 gatcacggca tgatgctgct tattttttcg gcattcatgt ggcgttattt cggcaaagtt
    46021 gcaggtctta tcgcccttat tatttttgtg gtttttgcgt ttcccagagt aatgattggc
    46081 gcacactggt tttctgacat cattgtcggt tcgatgaccg tgatattgat cggtttgccc
    46141 tgggtgttgc tgacgccatt aagtgatcga ttaatcacct tttttgacaa atcactaccg
    46201 ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa ttaacatcat caggtttatt
    46261 tttatagtga ggataatcct gatgatgcgc accgtgcttt catccatcga aagcaaaaat
    46321 cattctctaa gtaaatgaat ggattgcatg cgtttcactc aatcgtactt taattgacca
    46381 atcccgctta ttaactttct gtatcacttt ttcttataaa aaatcatgta aaaccgctcg
    46441 ccaagaccgc accaatcggg taatctcgaa ctcgttttgc ctcggcggta gattatcctc
    46501 acagcatata attttgtgcg ttagtccaca gatttggcct taaggaattg tttcaacatg
    46561 cccaggtaat tagtctcgtg tcgcttggca tttttttata acgatatttg tcgttaagga
    46621 cttcaaggga aaacaaacaa catggtcaaa tctcaaccga ttttgagata tatcttgcgc
    46681 gggatccccg cgattgcagt agcggttctg ctttctgcat gtagtgcaaa taacaccgca
    46741 aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa catcatcact gcaagcttct
    46801 caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag
    46861 tatgctgact ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa aggtatcgat
    46921 tgttctggtt tcgtacagcg tacattccgt gagcaatttg gcttagaact tccgcgttcg
    46981 acttacgaac agcaggaaat gggtaaatct gtttcccgca gtaatttgcg tacgggtgat
    47041 ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg gtatttatat cggcaacaat
    47101 cagtttgtcc atgcttccac cagcagtggt gttattattt ccagcatgaa tgaaccgtac
    47161 tggaagaagc gttacaacga agcacgccgg gttctcagcc gcagctaata aaccgtttgg
    47221 atgcaatccc ttggctatcc tgacgagtta actgaaagca ctgcttaggc agtgcttttt
    47281 tgttttcatt catcagagaa aatgatgttt ccgcgtcttg atccaggcta tagtccggtc
    47341 attgttatct tttaaatgtt gtcgtaattt caggaaatta acggaatcat gttcatacgc
    47401 gctcccaatt ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt gatgattgcg
    47461 atactggtga gctgccttca gtttttggtg gcctggcata agcacgaagt caaatacgac
    47521 acactgatta ccgacgtaca aaagtatctc gatacctatt ttgccgacct gaaatccact
    47581 actgaccggc ttcagccgct gaccttagat acctgccagc aggctaaccc cgaactgacc
    47641 gcccgcgcgg cgtttagcat gaatgtccgt acgtttgtgc tggtgaaaga taaaaaaaca
    47701 ttctgttcat ctgcgaccgg tgagatggac attccactca atgaattgat tccggcgctc
    47761 gacattaata aaaatgtcga tatggcgatc ttacccggca cgccgatggt gccgaacaaa
    47821 cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa atagcggcgt ctttgccgct
    47881 ctgaatctca acctgacgcc ttcactcttt tatagttcac ggcaggaaga ttacgatggc
    47941 gtcgccctca ttattggcaa tactgcgcta tctacctttt cttcacgttt gatgaacgtt
    48001 aacgaattaa ccgacatgcc agtccgtgaa actaaaattg cgggcattcc tctgaccgtt
    48061 cggctttatg cagatgactg gacatggaac gatgtgtggt acgcattttt actgggcggc
    48121 atgagtggaa ctgtcgttgg cctgctctgc tattacctga tgagcgtacg tatgcgcccc
    48181 ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt acgtggcgta tcaaccggtg
    48241 gtggatacac aagctttgcg agtaacgggc ctggaagtac tgctacgctg gcggcatcct
    48301 gtcgcgggag aaattccccc ggatgccttc attaactttg ccgaatcgca aaagatgatt
    48361 gtgccgctga ctcagcacct gtttgagtta attgcccgcg atgccgcaga attagaaaaa
    48421 gttctgccgg taggcgtcaa atttggcatt aacattgcgc cggaccatct gcacagcgaa
    48481 agctttaaag cagatatcca gaaactgctc acttccctac ccgcacacca tttccagatt
    48541 gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag aagccacaca actcttcgcc
    48601 tggctgcact cggtcggcat agaaattgct attgatgact tcggcaccgg gcacagcgcg
    48661 cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac
    48721 gccatcggta cggaaacgat cacttccccc gtacttgacg cggtgctgac gctggcgaaa
    48781 cgcctcaata tgctgacggt tgctgaaggg gtcgaaacgc cggaacaggc gcgatggcta
    48841 agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac
    48901 gattttgtcc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc
    48961 cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg
    49021 ttgcagatga ttgtgcgcat actgctgctg tttatcgctc tgttcacctt tggtgcgcag
    49081 gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg gtacgcgttt
    49141 aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca gataacgttg
    49201 tcagccctcg gtaccttcga taatttcaac cgctatgcac tgcgcggcaa cccgggcgca
    49261 cgcaccgagc agctgtacga cacgctattt acgacttccg atgacgaacc aggcagttat
    49321 tacccgctga ttgctgaaag cgcacgctat gctgacgatt attcctgggt ggaggtcgct
    49381 attaatccgc gcgcccgttt tcatgatggt tctcccatta ctgcccgcga tgtagagttt
    49441 acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc
    49501 accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa
    49561 gatatgttga gtctgttttc gctgccggta tttccagaaa agtactggaa ggatcacaaa
    49621 cttagcgatc cgctcgccac gcctccgctt gccagtggtc cgtaccgaat tacgtcctgg
    49681 aaaatggggc aaaatattgt ctattcccgc gtgaaagatt actgggcagc aaacttaccg
    49741 gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat
    49801 gtcgcctttg aagcgtttaa agcaggtgct tttgatttgc gtatggaaaa cgacgctaaa
    49861 aactgggcca cgcgttatac cggtaaaaat ttcgataaaa aatacatcat taaagatgag
    49921 caaaagaacg aatcagccca ggatacgcgc tggctggcgt ttaatatcca acgtccggta
    49981 ttcagcgatc gccgggtccg cgaagcgatc accctcgcct ttgactttga atggatgaac
    50041 aaggcgttgt tttacaatgc ctggagtcgc acgaacagtt attttcagaa taccgaatac
    50101 gcggccagaa attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat
    50161 ctaccgccag aagtcttcac acaaatctac cagccgccgg tatccaaagg cgatggctac
    50221 gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aagcgggctg ggtgctgaag
    50281 ggtcagcaac gcgttaatgc cacaacgggt cagccactca gctttgaatt attgcttcct
    50341 gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc
    50401 aacatggaca ttcgcaaggt ggataactcg caaatcacca accgcatgcg cagtcgcgac
    50461 tatgacatga tgccgcgcgt atggcgggcg atgccgtggc ccagttccga tttacagatt
    50521 tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt
    50581 atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attactgccg
    50641 ttggggcgag cactggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac
    50701 atggcggaag accgtctcgc ctggtgggat aaattctccc aacccgctgt acgccctgtt
    50761 tacagcctgg gtatcgatac ctggtggtat gacgttaata aagcggccaa actgccgtca
    50821 gccaagcaac agggagagta gatgggcgct tatctgattc gccgtctgtt gctggtgatc
    50881 ccaacgttat gggcgattat cactatcaac tttttcatcg tgcaaattgc gcctggcggt
    50941 ccggtcgacc aggccatcgc cgccattgag tttggtaatg ccggagtatt acccggcgca
    51001 ggcggtgaag gcgttcgcgc cagccatgcg caaacgggcg tcggtaatat cagcgacagt
    51061 aattaccgtg gcggacgcgg attagatcca gaagtgatcg ccgagatcac tcatcgctac
    51121 ggtttcgata agccgctcca cgaacgttac ttcaaaatgc tctgggacta catccgcttt
    51181 gattttggtg atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg
    51241 ccggtttcca tcaccctcgg attatggagc acgctgatta tctatctggt gtcgattccg
    51301 ttaggcattc gcaaagctgt ttataatggg agccgctttg acgtctggag tagcgcattt
    51361 atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc
    51421 gcaggcggca gctatttcga cctgttccct ctacgcggcc tggtttccgc taactttgat
    51481 tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg
    51541 gcgacggtga ttggtggctt tgcggcgctg actatgctga caaaaaactc attccttgat
    51601 gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt
    51661 ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg
    51721 tttatcagca tgttttttac cggctcgctg ctgattgagg tgatgttttc actcaatggt
    51781 cttggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc
    51841 ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg
    51901 ctagtcgatc cgcgtataga ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc
    51961 aggcccgttg ggcgcgtttt cgccataacc gtcgcggcta ctggtcgtta tggattttcc
    52021 tcgttttgtt tggtttgagt ttgtgttctg aacttatagc caacgataaa ccgttgctgg
    52081 tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa ttacagtgaa agcgattttg
    52141 gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa
    52201 ataatggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta
    52261 ccgataagcc cttcccttct cccccctccc ggcaaaactg gctgggaacg gatgccaacg
    52321 gcggcgatgt gctggcacgt attctctatg gcacgcggat ctcggttctg tttggcctga
    52381 tgctgactct ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggctattacg
    52441 gcggtaaagt cgatctctgg gggcaacgct ttattgaagt atggtcgggg atgccgacgc
    52501 tgtttttgat tattttactt tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa
    52561 ttactgtctt gtttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgta
    52621 cccgtaattt cgactacatt cgtgcggcgc aggcgcttgg cgtcagcgat cgcagtatca
    52681 tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct caccttttta ccgtttattt
    52741 tatgtagttc gatcaccacc ctgacctcac tcgatttcct cggcttcggt ctgccgctcg
    52801 gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa taaccttcag gctccgtggc
    52861 ttgggatcac cgccttcttg tcggtggcga tattattgtc tttgctgatc tttattggtg
    52921 aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta
    52981 gcgattgaag atttgtcggt gggttttcgc catcagcaaa ccgtacgtac agtagtcaat
    53041 gatgtttcac tacagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca
    53101 ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc catccccgcc ggttgaatat
    53161 ctctccggcg atattcgttt tcatggcgaa tcgctgcttc atgccagcga tcaaacgttg
    53221 cgcggtgtac gcggtaataa gatcgccatg atttttcagg agccgatggt gtcgttaaat
    53281 ccattgcata ccctggaaaa acagctttat gaagtgcttt cactccaccg cgggatgcgt
    53341 cgggaagcgg ctcgtggcga aattcttaac tgccttgatc gcgttggtat ccgccaggcg
    53401 gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacgcca gcgggtgatg
    53461 attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc aaccaccgca
    53521 ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagctg
    53581 aatatgggca tgctgtttat tactcataac ctcagcattg tcagaaaact ggcccaccgc
    53641 gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata acgccgctac gctatttgcc
    53701 tcacccactc atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgatccagtg
    53761 ccgttgccag aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc
    53821 attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt
    53881 tttacgctac gggcgggtga aacgctgggt ttggtaggcg agtctggttc cgggaaaagc
    53941 acgacgggac tggcgctgct gcgactgatt aattctcagg gcagcatcat ctttgacggt
    54001 cagccactgc aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg
    54061 gtatttcagg atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag
    54121 gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca acaagtgata
    54181 gccgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc
    54241 tctggtggtc agcgacaacg tattgcgatt gccagggcat taattcttaa gccctcgctg
    54301 atcatacttg atgaaccgac atcatcactc gacaaaacgg tacaggcgca aatattgacg
    54361 ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag ccatgatttg
    54421 cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc gacaagggga agtagtggaa
    54481 caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg agtatacgcg tcagctactg
    54541 gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc
    54601 aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg
    54661 ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcttaacg cctggcttag
    54721 tttatacatc accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag
    54781 gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc
    54841 agcagaccag cgatagttct gcggcagtcg atggacgatt gagagttgta atgaagtcac
    54901 ttattttccc cggaagcaca tttacttcac aatttattca catcaatttt aacacatcat
    54961 caacaatccg tctttataca gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg
    55021 ctctgattta agtaatatct gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt
    55081 ctgcgatcta actcaacctt tttaactaca atgatgtgac tttttacata aattgatttt
    55141 acataaaata aacatatatc gggggaatga tagatttgtg gttgacatgc atcaacaaag
    55201 gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt ttttcggccg actggcgtac
    55261 agacagaaga gaatggagct ggttgcgcag aatgcaattg accaaatcat cggccatgca
    55321 gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc aaaacggaag
    55381 gttcctgcca gcgaagatgc cgttcccgcc atatggggaa actcatcaag aatgaccgcc
    55441 atcgcattgg atgacaccat cgacacgcag cccacaaacg ccgcaacgcc aaccaccagc
    55501 gaccagaatc ccagccccag tagcgcactg atgaccatcc acgctgccat aataaactgt
    55561 atccacaacc ccgagcggaa catatttaac gcgccaatac ggcggacgaa gcggctatta
    55621 aagatggtca tcacgaacag gaaaacaatg tttagtgcaa agtaataacc aaagttttcc
    55681 ggcgcgacgt ggttaatttc aatgtagaca aacggtccgg cgcttaagaa cgagaacatc
    55741 ccggcaaagc tgaaaccact ggccagcata taactcagga cacgcttatg acggaacagc
    55801 gccgcaaagt taccaatagt ggtacgaatg tgaaaaggct ggcgacgctc cggtggtaag
    55861 gtttctttga tcagaaagaa gatcattgcc gaagccagaa tcgccgccac ggcgaggatc
    55921 cagaagatgt aatgccagct cagccacacc agcacccagc cgccaactat cggtgccatc
    55981 agcggtgcaa tggttgtcac cagcatgaca aacgacatca tccgcgagaa ctcttcttta
    56041 gggtagatat cacgcatcaa agcgttgata accacgctgg ccgcagccgc cgccagcccg
    56101 tggaagaaac gcatcacaat cagctgatcg atggtttgtg ccaacgcaca cgccaccgcg
    56161 gcggcggcaa acaccagcgt accgccaaga accaccggct tacgcccgaa gctgtctgcc
    56221 atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact gagggtcatc
    56281 tgcgtactgc ccgccggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac
    56341 atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac
    56401 gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag ttagacgacg
    56461 ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc
    56521 gtaatgccgc caatacgttc acgatgcagc tcaaccacat ggttacccac ggcggcgaac
    56581 atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta
    56641 atcacttcca gcaccgccgg cttagtgaga tctttttcgt tatgcagctg cacgccttta
    56701 gcaaattgct ctgccgtatc gtcagctacg ggcgattcca gcgtcaccag ataggtcttc
    56761 tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc tgtcattagt
    56821 accagaccgg tggtatcgat atccagccgc cctgcggcgt gcagtttcca cgccactggt
    56881 tcatccagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacatagccc
    56941 tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg gttgccatca
    57001 taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat ttcgccatcg
    57061 acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc
    57121 tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac ggaggtcggg
    57181 cattgacccg aaaatctgaa ctgttgtctg cccagtatag cggtctaata acttccctca
    57241 agggaaaaag aaccgtggca tactattagc agaataatct acctacgcga gaccatgatt
    57301 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    57361 cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg
    57421 gaacttgcgc ggctggctcg tggtcgcgta ctggtgctgg cacacgttaa agaactggtg
    57481 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    57541 ctaaaacgca aggagagcca cggtaaagtg gtatttggca gcgtgcagtc ggtcgcccgt
    57601 aatcttgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    57661 ggtgatgacg aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc
    57721 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ctggatctac
    57781 cagttccatt atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt
    57841 tatgagctgc cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg
    57901 gatatgccag tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctgttc
    57961 agcgaggccg atctcaaccg cgagctaaaa aaacaacaac gtattacccc gcacatcatc
    58021 agccagatta tggagtttgc cgaaaagcgt aaaggagtga tgattttcgc cgcgacggtc
    58081 gaacacgcaa aagagattgt gggattacta cccgccgaag atgctgcact gattactggc
    58141 gacacccccg gcgctgagcg cgatgtgtta attgaagatt ttaaagccca gcgttttcgc
    58201 tatctggtca acgtcgcagt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    58261 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    58321 cgtctcgctc cgggcaagac tgattgttta attcttgatt atgcgggtaa tcctcacgat
    58381 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    58441 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    58501 ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc
    58561 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc ggaaaacgat
    58621 attgccgccc gccgctgccg cgaatgtgac accgtactgg ttgatccaga tgatatgtta
    58681 aaagcggagc tacgactgaa agacgcgctg gtattacgct gtagcggcat gtctttacag
    58741 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg
    58801 gatgtgagtg agcgtttccg tctgcaaacg cccgcccagc gaactgcctt cgagcagctt
    58861 tttatccgcc cgcatacgcg cacaccgggc atccctctgc gctggatcac cgccgccgat
    58921 atcctcgccc agcaagcctt attgcgacac ccggattttg tcgttgcccg catgaaaggt
    58981 cagtactggc aagtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    59041 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg
    59101 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    59161 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    59221 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    59281 agaagcaccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    59341 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    59401 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    59461 ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggttttttgt atctgcagat
    59521 tatgcctgat tacgatattg ctattttttg caggccagat aaggcgttca cgccgcatct
    59581 ggcattcata acacaaaacc gattaattgc cgccggacgt ccggcgctgc aattgatcgc
    59641 gcaaattcgg cggcgtgcct ttaatagtca gggtatcggt cgccggatcc cagaaaatcc
    59701 gttcgcccaa cagcatcgca tcaaaattaa tagtcaagcc accgccgcta ccggcaaact
    59761 tcgtcaactg acgcagtgtg ctacggtctg ccgggaagct ctcttccagt tcgtagcctt
    59821 tttcagccgc aaactccgtg aagctcactt cgctgacgcc tgccagctct ttcgacagcg
    59881 attccagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct
    59941 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg
    60001 cttgcaacag cccacggttt tgcgctttcg cgttcaatcc ttcgctggcc ccgaggaaat
    60061 ccataaagaa atccgccact ttgcgcccta ctctcccttt caggaaagtg agatagcggg
    60121 tcgactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat
    60181 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacgcgc atactgctca
    60241 ggttgctcag aactgccacc agcaaatact ccaccgccag atagcgatag tggcaaaaca
    60301 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccggttg
    60361 ccgcgcggct aaatgccaga aagtcctctt caccctgacg ctgtaagcgc agcgtctgcg
    60421 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    60481 gcagttctgc taccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca
    60541 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    60601 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    60661 gacgcaataa aagatggcgg tcattttaca gaaggcggcg taacgagaat gatttaacgc
    60721 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    60781 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    60841 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    60901 taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    60961 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    61021 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    61081 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    61141 agccgttacc tgtttatcgc cgactggccg accacgcttg ctggtcgcat ttattcctac
    61201 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    61261 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    61321 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    61381 cttaatccca tcgtctggca actggttatc aacccagacg aaaatgagat ggcgcgcgac
    61441 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    61501 tggagctggc aaaagctgcg cagcctgacg cgtcgtcggc gcttcgcgcg cccgctggcc
    61561 gcattcttat ttatcacctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac
    61621 ttctatcgcc ctatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg
    61681 cgacgttttc ttgagaagca tggcctgctt gatgcgcagg agtatcaacg ccgtcttatt
    61741 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    61801 gatatgggca ctgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    61861 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    61921 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggcatctcg
    61981 ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    62041 aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat
    62101 cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag
    62161 accgccacgc agtggatcaa ctggctgggc cgctacgcac aagaagataa ccgctggttc
    62221 tcgtgggtct ctttcaatgg cactaacatt gacgacagca atcagcaggc atttgcacgg
    62281 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    62341 cgtgattctg gcaaactgga caacacggtg gtgattatca ctgccggtcg gggtattcca
    62401 ctgagcgaag aggaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg
    62461 attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg
    62521 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    62581 ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttaccgcagc ggataacgat
    62641 acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc
    62701 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    62761 caagtactga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg
    62821 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt aacagccacg agtcggcacg
    62881 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    62941 gtgccgacca aaaatcccaa aaaaaaccaa cccttacggt tggttttttt atatctgcaa
    63001 ttaattcgat aaacagaccg tgacacatca cagcctgttt attttctgtt atcagaacgt
    63061 ccagaccaca cccgcctgag tattccacgg cgattctacc cctgcgccat tgccatagct
    63121 gactgacaaa tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac
    63181 gccactggtt ccagccacat cattattaaa tttgccatcg tcattaactt tcacctggtt
    63241 agaatcggcg tattcctgac gcacggcggc tttcagccag ggttccagcg tcgtaccgtt
    63301 ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt
    63361 tcccacgtcc gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc
    63421 ggtaaaggcc agatagggtc taacactcca caatccgtca acccaacgga acccgctctc
    63481 aacatgagca cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat
    63541 cttgccatgg atggtgttgg caaaacggtc aactttcacc accccatcaa cataggcacc
    63601 gttctgatgc tcccaaccgg cataagcccc cagggtatag ctatcgatat taccttttcc
    63661 gccgcgatca aaaccaatat cagaatgaga gtaaccaaag aacaagccgc gaattgtact
    63721 gctttcttca cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc
    63781 aaaaccagct cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat
    63841 cgccgtatcg taactaacgc cttttacgct accaagacgc tcacgcacgg tgtccagttc
    63901 tgcatcaaat accagcggtt gtgctgccgc catatttagc acatcagtgg ttgaaggggt
    63961 aatttgcgcg cgattctctg ccagactcca gctatggtta ccattatcca gcaaggtata
    64021 ttcatacgta ccgatatcaa caacgcctcc ggcattgccc aacgtaaatg cagcatcacc
    64081 gccgcccgtt gttaccagtg taaggctatc tcctgctgcc gggctggcac cggtgtccgt
    64141 cacgaatatt ttgaaatcac ctgttgcctg accggtgacg ttgagctgat cgctctgatg
    64201 attagccata tcggtacgca tataaaaatt gccgtttccc gacagggtat tggtggtcaa
    64261 cgtaatgaaa ttttccgcac ttgccgtcac tgatgaacga gtcactggct catccatcat
    64321 aatcacattt gcgtcagaca tcgcaaggtt ggctaagttg aggagggtgt tttggttcgt
    64381 cgtagtgata tcggcaatga tggtccatac gctattttcg agcgaaacgt ccgctttgct
    64441 ggtatccacg gcgccatgcg ttctaaaaga agcgccctca gaaaccacta ccttacccac
    64501 caacattgcg ggtgcataat ttatttgcgg cttcatgacc tcaagaatgc catcattccc
    64561 tctgactgaa acgttaacca ttgtgccagc ccagacgtta gcgcggccat tgttgataac
    64621 catattttca gagatggctt ttgaggaata cgtgatactt ccattctgat ctgatcgacc
    64681 gagatcatat accgcatttg cctgcactct cgtcccagta tcctttccca gcgattgcat
    64741 agtggcatgc tcatcaagga tagtgtcaat ggcctgcgtg tcctccataa caataagccc
    64801 ggaaccatca tccagttcat aattttttga cacaccatct tttatactaa attggccttt
    64861 actgtttgta ccactcactt ccagcgcatt cgttgagaca attaattttc cgcctgcttt
    64921 tttatcaacg ccagttgcag tcccgccatc cataacctcc agtaagccgc cactatctac
    64981 agtagtatta taagcgaagt cattttcttc aactcgcaaa cttccgccgt tttccaacag
    65041 catattgttt gcaattccat tcttgatatc gaactgaccg agacggtttg ttccgaatac
    65101 ctccatgacc cgcgtggttg ctttaatagc accgcctgct ttctgatcta ccgcaatcgc
    65161 taatcccccc tctttaacct ccagtaatcc tcccttttcg atagcggcaa ggacggtaat
    65221 cccatctttt tcaacgatta agcgcccgcc tttaatcgtg ggatcattcg ccgcgccata
    65281 aacctgtagc acaccattat tgagggtagt ctggtttgcc gcgccaccca tttgaacaat
    65341 ttgataacca tcatttaata cggagtattc tgcggtgcca ttcatttcaa tgtactgata
    65401 cccgccgtta atttcagtat tactacttat accaaattct ttaatatgct gttctccgcc
    65461 aggattgatt ttcgtattat tggcggtccc ataaaccagc tggatatctt tttccaggac
    65521 aacaccatca actgtctcac catactcaac tgttgatgcc aatgctatgg gtgaatataa
    65581 cgctgtagcc accagaacag atattgcgga acgacaaaga gaaacagaac cagattgatg
    65641 cattgagctt tcatcctatg aaattaattg ctgttaaaag cattgggtac agaaaatacc
    65701 catagctcca tacccggaat cagtttttaa aaactgttta aagaaatgca caagtattgt
    65761 gattgatttt ttagttgttt ttcttgatga gaagctgatg caaaattccg tctttataat
    65821 gaaaatgatg ccaaagcgaa cggcaaggtt gtagttttca ctacatgtcc atacataaaa
    65881 tggggtaaca ttcacgcgcc tggtagcgtt accaacgcta cgctcaaaca taatgattct
    65941 aataaaacct caggagacta ctatgcctga agcaacgcct tttcaggtga tgattgtgga
    66001 tgatcatcca cttatgcgac gcggtgttcg tcagttactg gagcttgatc ctggctttga
    66061 agtggtcgcc gaagcgggcg acggcgcgag cgcgatcgat ctggcgaata gactggatat
    66121 cgacgtgatc ttgctggatc tgaatatgaa aggtatgagt ggcctggata ccctcaatgc
    66181 cttgcgcagg gatggcgtta ccgcgcaaat tattatcttg accgtatcag atgcctccag
    66241 cgatgtcttt gcactgatag acgcaggcgc agacggttat ctgttgaaag acagcgaccc
    66301 ggaagtgttg ctggaagcga ttcgtgccgg agcgaaaggc agcaaagtct ttagcgaacg
    66361 cgtcaatcag tacttacatg aacgtgaaat gtttggcgcg gaagaagatc ccttcagcgt
    66421 gctgacggag cgcgagctgg atgttctgca cgagctggca caggggctgt caaataaaca
    66481 gattgcctcg gtgttgaata tttcagagca gacagtaaaa gtacatattc gcaatctgct
    66541 gcgcaaactc aatgtccgct cacgcgtggc agccaccatt ctgttcctgc aacaacgcgg
    66601 ggcacaataa aaatagcccg atggcatcac catcgggcct ctttttattt actctcctgc
    66661 ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa tcaccgcacg gcgagtatcg
    66721 ttggcaggta agagtttcaa catcatctcc cacgcagcaa cggcttcgcc aaatcgctgc
    66781 tgctcaaagg cattaaacgc atacatgctt aacacacgaa tattgctatg gtccgttctc
    66841 accaactggc gcagcagttc gccgccgagg cggttgtcgt tgggatcaga tgaacgtgtc
    66901 aacgcttcag cataacccag tgcggcatcg ctgtttttcg gatcgaggcg atacgcagta
    66961 gcgtatgcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg acccaacata
    67021 atccaacctt ctatatctcc cggatttttt tgcagttgag tacgcatccc cagcgcaagg
    67081 cgcgacatct cttcttcgtt gagcggatcg gctttcggat ccagcgccct gtccagcaac
    67141 gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt gccagtctgg
    67201 tagtagctga cgccagccac aattaacgcc accacaatac ccggcagata aacaacatat
    67261 ccggcacgct taccttccgg cacgctttgt tcaggaaacg cttccagcac cacgcgtacc
    67321 cgacgccgcg agcgggcgta aatgacccaa ccgccaatgc caatagccac tactggcagc
    67381 acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa gttgccgtaa
    67441 cgcgccacca tataatcgac aatctctttc ttacttttac cttcctgcat cagttcatac
    67501 actttctggc gcaggtcggt ggcaatcatc gagttggaat cggcaatgct gttattctga
    67561 catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg tgcttcatct
    67621 ttaaactgca acacgtcgat ggtcgccagc gctgagccgg agatcatcag catcagcacg
    67681 cccaataaaa acctcattgt gcggcctcct tactgtattt ctcccacagc ggcttgatct
    67741 cttcttccca gacgcgagga ttcagatcgc ccgcatggcg atagcgaatg atgccgttgc
    67801 cgtcaataag gaacgtttct ggcgcgccat agacaccgag atccagccct aacatgccgt
    67861 cgccatcaaa caggcttagc gcgtaaggat tgcccagctc tttcagccag ctgattgcct
    67921 tctggcgatc gtctttatag ttcatgccga ccacgcggat gccctgcgca gaaagctgat
    67981 tcagatattg atgttccgca cggcaggtcg gacaccaggt cgcccagacg ttaagcagca
    68041 ctggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg tccagtgatt
    68101 cgaggcgaaa cttcggcaca ggcttgccaa tgagcgccga ttccagattg gtcggatcat
    68161 ccccttcggc attacgcgcc aactgccaca gcagcgccgc ggcaatcgcc aggaagataa
    68221 tcaaaggaat taacaatact ttgcgcttca tacagcctcc ggcgcagttt tctgcgggtt
    68281 cacgcgctta cgatagcgag gatcaaacag gcacagcagc ccgcccagcg ccatcatcag
    68341 cccgcccgcc cagatccagc gaacaaacgg tttgtagtaa agacgaacag cccaggcacc
    68401 gttttccagt tcttcaccga gtgccgcgta caggtcacgg gtgatgccgc cgtcaatcgc
    68461 cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttccgcat acagcaccgt
    68521 ttccggcttg ccatctcgcg ttacgccgat agtcgccaca ccgccgcgcc agttcggacc
    68581 agtcacctct ttaacatcac ggaaggtgaa gcgatattca tgaatatcga cgctatcgcc
    68641 ggacttcatg cgcacatcac gctcaacgct gtagttctga ctgaaagcaa taccaacaat
    68701 tgtcactgcc agccccaggt gagccgccac catcccccaa tagctgaagg tggttttcgt
    68761 gccgcgtgaa atacgcagcg ccgcttctgc aattgccagc accgcaatcc agcaggccat
    68821 tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg gcaacagcag
    68881 agacagcacc agcgtagaga tgaaggcgat aatcaataaa ttgcggattt tacgtgggcg
    68941 atcgcgcccc cagcgcacca gaggaccgac accaagcagt agcgcaaacg gcaccatcag
    69001 ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca gccccagttg
    69061 tttgtgcacc agcggcagca gcgtccccag caacaccacc agcatcgcgg cgaccagcaa
    69121 aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta cgcgtgagcg
    69181 aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca gcaccataaa
    69241 ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg ataccagcac
    69301 gccggaacgc acgaggaaag tccccagcag acacaacgag aaggcactga ttgccagcag
    69361 taatgtccac gctttgaagc tggcacgctg ttcagtcacc gccagcgagt gcatcaacgc
    69421 agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga accaccagcc
    69481 accccagccg agttcgtaat aggcccatgc ggaaccgagc acgatgccga gcgtcaggaa
    69541 gatccacgct gccagcgtcc acggacgagt aaaacgcgca taagtgctgt ccagacgccc
    69601 gctcaacaaa gaagcaatgg caaaagcaaa cgccaccgag aaacccacgt accccatata
    69661 gagcagaggc ggatggaaga tcagccccgg atcctgcaac agcgggttaa gatcgcgccc
    69721 ttcaatcggg aagttcggca gcgtgcgtga gaatgggtta gaggtaaaga gaatgaacag
    69781 caaaaagccg acgctgacca tccccattat cgccagtacg cgggccacaa tatccagcgg
    69841 aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg ctcatcagca gcacccacag
    69901 cagcagtgag ccttcatgtg caccccaggt agccgccacg cgataccaca ccggaagctg
    69961 ggtattggag ttgctggcaa cataggtgac ggtgaagtcg ttgaccacga aagcattgac
    70021 cagcaccaga aatgcgccag ctacagacat aaacagcagc caggcaaaca agcgggaaga
    70081 cgccatcatg cgcgcatctc cgcgcgccac gccccatagc ggatacacag acagcagcag
    70141 cgcaattcct agcgccaggc acagcagccc gttaccaatt tccggcatca tgatgctggg
    70201 tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc aacttctggc
    70261 ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg cgaggatatg attgcctttt
    70321 tccagctcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg caaaatgcct
    70381 tcgtaagaga catccactga gccttcagca tcgtaaatgg tgaacgttac tttcagcgaa
    70441 ttgggatcgc gctgcacgct acccggcatc accatcccgc caacgcgcag acgctgaccg
    70501 acttccggca tctgctgagt ttcacgcttg ccgtagagaa tttcccccgg cgtataaaag
    70561 agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc cagccctgcc
    70621 aacacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca gcctcctgct
    70681 gttgtgcagc acgtaaacgc gcctctcgcg cccgctgttg cgccacgcta cgcagaattg
    70741 cgcgatgctg catcaccgag tgcacgacca aaaccaccag cggaataacg gtcatcacca
    70801 ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc caggaagcaa
    70861 atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg gacggcgttt
    70921 ttccatcagc aaaatcaaat tacgcatccg catcagcgtc agcgtggcag acagcagcag
    70981 gaagccaaaa atcgaccagc gcagcggcga acgcatcgca ggatcgatac tttgctgcat
    71041 ccgcgttgat ccctgatgca gggtgttcca ccactccacg gagtaatgaa taatcggcag
    71101 attcaccacg ccaatcagca ccaggatacc tgccgcacgg cccgccagac ggcggtcgtc
    71161 gaaggcgtgc cacagggcaa tcacacccac atacaaaaac agcagcacca gttcagaagt
    71221 cagacgtgca tcccataccc accaggtgcc ccacatcggt ttcccccatg cagagccggt
    71281 aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca ccgccaggtt
    71341 ggccattttc atctgccaga caaggccaat aaaagccgcc accgccattg atgcataaat
    71401 gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc tatttccctg
    71461 ctgataatca gccggagcaa agccgaatcc ccagatccag ccgacggtaa gcacgaccac
    71521 actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc gtggtgggat
    71581 cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca aaaatcagta
    71641 atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg acttaatgtc
    71701 gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc cgtcaacggg caaatgcata
    71761 gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag cggtaacacc
    71821 agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac gcccggcgca
    71881 ccaagaaagc caagcgtagg ggttcccagc agtagcgtta acgccatcac ttgccagcca
    71941 taaacatcca ttcccagtag cattgctacc aggggcgaaa ggatgagtaa cggcagaccg
    72001 gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg taacggcaac
    72061 aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg ttccagcgcc
    72121 agcagggatg aaagcagcgc ggcaacccag ataatgcccg gtgcaatacg tgccagcagt
    72181 tgcggttccg gaccgatact gagcggaaaa agggtaatta caatcaggaa gaaccacagc
    72241 gggttggcga tttcagcgct atggcgaaac gctacgcgca gctcaagacg gaaaatgcgc
    72301 cagaacatca tacggccctc gtttgcgtca gtgaaatgcg gcgaatttta ctttcagcaa
    72361 cgttgagcgg ctggtgggta gtgagaatca caatcccccc ctgctctgta tgctgcgcca
    72421 tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc ggtaaaaggc tcgtcgagga
    72481 tccataacgt ggcacgggtc agccacagac gcgctaaagc gacgcggcgt tgttgcccgg
    72541 cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc gccagggctt
    72601 ccagacattg tgcggtatcg ccatcgcgat gataaaagtg cagattttct aacgccgtca
    72661 gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg tagctgtcgc
    72721 gtacctgatg caagggctgc ccttgccaga gaacctcgcc tgcgtcaggg cgagacaacc
    72781 ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg atttgtaccc
    72841 actctcctgc gttcagcgta aatgacaagc cactaaataa ggttcgttca tcccgctcac
    72901 aaagtaactc tctgacttca agcataccca cgcaataacc ctgttaaaaa cctggctcga
    72961 cttcacgcat atcggggagc ttgtgcgcta tccctttatg gcaatcaata caggtttgcc
    73021 catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg gttgtatcca
    73081 tatactcgaa gttgtgacag ttacggcact cctgcgagtt attgtccttc atgcgccgcc
    73141 actcattctg tgccatcgtc agacgatgag cttcaaattt ctgcggcgtg tcaataacgc
    73201 caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc ttcggcacaa
    73261 actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg ttgttgtagt
    73321 gcacggaatc catgtattcc tgatacaccg tgttacgcat ttcgtggcag ctaatgcaga
    73381 actcttcggt attggctttt tccatcccgg tgttaaagcc gccccagaag acgatgccgc
    73441 cgacaaaacc gatcaacagc agcgtcccca gcgccagacg gctgggggta cgccaccatt
    73501 tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata acctcttatt
    73561 tcccgtaacc ttttgatggg gtaaaggtat tccccacgat tggcgcggta tcggcctgcg
    73621 gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc actttgccgt
    73681 cgctgtccat aaagtgagta ggactgatac gcggcgcgcc agtggtgcga tagctttcga
    73741 caccgtggca ctgcaagcag cggttggtat tggtcgttac ctgataacct tcaacgctat
    73801 gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc tcttttggca
    73861 tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa tcgacgccat
    73921 tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat tgacacagcg
    73981 ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg tagttattgt
    74041 aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat cgcggctggt
    74101 gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg gttccgggca
    74161 aacatgaaaa caatccatac agcggttaca tttctgccga tcgctggccg caacggtaat
    74221 cacacctttg ctacccagca cgccatacag cgcgccaacc ggacaaatgt gcccgcacca
    74281 gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca gcgcgccgct
    74341 gccgaagccc attaccaggc tacgccccat cagggaaacg gggttgatcc attcccagat
    74401 gagcgtgcca gttaaggctg atcccaccag aatgaccacc aacagcacgt agcgaatgtg
    74461 gcgagggatc gtcgcagact gattgaggtc aaagcgcctg cgtaaccagt tcgctaagtc
    74521 ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcctg ccagggcgta
    74581 gagcacggtg ataatcaccg caccggtcaa cgccaccgtg gcaggcagat gaccactggc
    74641 aagactttgc agcgtcatca gcggatcggt cagcggaacg gtgtcgaata acaggctact
    74701 gctgtagttg ccgtgcaaga tccacacacc aaaccacgga ccactcaaaa acatccccag
    74761 cacgaagaac tgacaaaggc gacgtaacac cagccaacgg tgactgcgcc accagccttt
    74821 tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc ccctccagcc
    74881 agccgaagcg gtaatggtgg cctaactccc ctttcgccag tgacagcggt aacaccttga
    74941 ttgccggttg ttccagcacg cacacttttt cgcacttacc gcaaccggta caggcgtcgc
    75001 tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg cgtgttgcgc tccagctcca
    75061 gggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag cgcagcccct
    75121 gaaagttgag acagttttcc tggtccacca gtaccgccag ccccatccgc gcgtcgtcga
    75181 tcgattcaat ctcacgatcc agcgcaccgc tcgggcacac tttggcgcac ggaatgtcct
    75241 cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc gacaggccag
    75301 aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac tgaccgcaac
    75361 gcacacaagc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc aaccgcacgc
    75421 cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca gccagcccgc
    75481 ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt ttcgctgacc
    75541 gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag tccgtctctt
    75601 tcgagagcgg atcggtcgcg tccagcgtta gtttgttaac cagctgtgcg gcgtcgaaga
    75661 acggcatgta caccaggccc tgcggcggac ggttacgacc gcgcgtttcg acaatcgaga
    75721 tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga tcgcgcgctt
    75781 tcgcatccag cgggtgaata aacaggaccg cttccgggaa ggcacggtgc agttccggca
    75841 cacggcgagt catactgccg gtgtgccagt gctccaggac gcgtccggta gagagccaca
    75901 ggtcgtactc ttcatccggt gcttccgccg ctggttcgaa cggcagtgcg aagatcaccg
    75961 ctttgccatc cggtttaccg tagaacttgt agccttcgcc cgctttcacg tacgggtcgt
    76021 taccttcgct gtaacgccac tgcgtttctt taccgttcac caccggccag cgcagaccgc
    76081 gcgctttgtg gtagtcatcg aacggcgcga gatcgtgacc gtgaccgcga ccaaaccatg
    76141 cgtactcttc gaacagccct ttttgcagat agaagcccag ctcgcgggat tcatcgttca
    76201 gctgatcttc cgccagttcg gataccggga atttgctcac ttcgggggtg gcatacagaa
    76261 cttcgtacag cgttttgcca cgcagttccg gtttcttcgc cagcagctct tccggccata
    76321 cttcttcagt tttgaagcgg cgggagaact ggactaactg ccagaggtcc gatttcgctt
    76381 cgcctggcgc ctgtacctgc tgacgccaga actgagtacg gcgttcggcg ttaccgtaag
    76441 ccccctcttt ttctacccac attgcggtcg gcaggatcaa gtcggcggcc agcgcactga
    76501 ctgtcggata cggatcggag acgatgatga agttgcgcgg atcgcgccag cccggcatac
    76561 gctcttcatt aatgttcggc ccggcctgca tgttgttggt acacatggtc cagtaaacat
    76621 tcagcttgcc gtctttcagc gcacggtctt gtgctaccgc atgcagaccg attttcgccg
    76681 gaatggtgcc gctcgggata ttccacttct tctcgcagat atcacgatgt ttctcgttag
    76741 ttaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc gcagtaccac
    76801 acgcggaagg ctgcccggtc agggagaacg gaccgcaacc cggctgggaa attttgccgg
    76861 tcagcaggtg caggttgtag accaggttgt tggcccacac gccacgagta tgctggttga
    76921 agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc tgcgccagtt
    76981 gttccagctg atcttttggt acgccagtca tttcagcggt tttttccagc gtatactcag
    77041 caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa cccggattct
    77101 tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc gtcgcccctt
    77161 tgcgcaggtt aacgtgcttg ctaaagaagt cctgatttat cgcattgttt tgaatgatat
    77221 agttggcgat gtagttcagg atcaccaggt cagattgcgg cgtaaagatg atgccgttat
    77281 ccgccagctc gaagctacga tgctggtagg ttgaaagcac cgccacggtg acgtcctggt
    77341 tagagagacg acggttagtg atgcgtgacc agaggatcgg gtgcatctcc gccatgtttg
    77401 cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc atcggctcat
    77461 ccataccaaa ggtacgcata aagccaacta ctgccgacgc catacagtga cgcgcgttcg
    77521 ggtcgatgtt gttcgaacgg aagcccgctt taaacagctt ggatgcggca taaccttccc
    77581 agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt tctttcaggg
    77641 cggttttgaa tttctcttcc atcacatcga aggcctgatc ccaggtgatt ggggtaaatt
    77701 cgccttcttt gtcatattta ccgtttttca tacgcagcag cggctgcgtc aaacggtctt
    77761 taccgtacat gatcttgggc aggaaatagc ccttaatgca gttcaggcca cggttaaccg
    77821 gtgcgtccgg gtcgccctga caggccacca cacgcccctg ctgcgttccg accagaacgc
    77881 cgcaaccagt accgcagaaa cggcacggcg ctttatccca tttgatggct tcctgctgac
    77941 caacaacggc gcgggcaacg cccggcacgc tgagaccggc agccgccgca gcggccgcaa
    78001 cggcgttagc tttcataaag ctacgacgac tgagtttcat ggtgtttcct caccttgctc
    78061 ttcctgctgg tgataaacca gcgacaccgc cagcacgccc tctacgttgc gtactgactc
    78121 aatggtttgg atcagcgttt cgctgtcttc tgcttccacc accacaatca actgaccgct
    78181 cggcgcgtcg ctgacagcaa cttcacagcc gggagaggcg ttcagttggg tgctgatgtc
    78241 tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa acttgccagt tagtgtgcat
    78301 ggagatactc cgcagttatg gctgataccg gacagctggc tgcacatgct ccacatccgt
    78361 tgcaaagttg gctattaagt tgcggctggt agatcccgga gagcgtcggg cgaaagataa
    78421 ttgccattgg ctcacagcta tcctgacagc ggcggcattc aactgactga tacgccaggc
    78481 acgcgtcccc aatggtgaac tgcaaatccc aggccctggt gtggcgcgga gaaaatagcg
    78541 attcggggca agcctgcgcg caggcgtaac agaagctgca ctcgttattt ttgaaattaa
    78601 cgctcggata gccgcccgcg ccgcgttgca gaatgttgtt ttcacaagca ttaatacagg
    78661 cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc accggaccag ggcggacgga
    78721 taccgttact ggctttgcgc cagcgaccag tgagtatgcc ccgacgggat gcatcaatct
    78781 tcacattgac cttccatcat taacgcgctc tgaaaattga gagcgaccaa ataaaccgca
    78841 taattaataa gccattttta tagccgctaa gatattaaag gatgtgtcaa agatgcatac
    78901 cccgatcgag gtaaaacctg tagcaggatc aaaagagtgg cgggaagcgt ggcaaaaacg
    78961 ggcttttgct cacatttcaa atggttataa acatatttat atagcgatta attcaccaga
    79021 gatatttctg ctggtttgtt ctctcatcag aatttaacac taaaagagca ggtaaaattg
    79081 tct
//
LOCUS       DAZGCQ010000007        76263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000007 DAZGCQ010000000
VERSION     DAZGCQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.007"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK07_000523"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK07_000523"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            120..195
                     /locus_tag="ACHK07_000524"
     tRNA            120..195
                     /locus_tag="ACHK07_000524"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Thr,seq:ggt)
     gene            235..350
                     /gene="rrf"
                     /locus_tag="ACHK07_000525"
     rRNA            235..350
                     /gene="rrf"
                     /locus_tag="ACHK07_000525"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHK07_000526"
     CDS             complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHK07_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO8100905.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHK07_000527"
     CDS             complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHK07_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8100906.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHK07_000528"
     CDS             complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHK07_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8100907.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3541..4566)
                     /locus_tag="ACHK07_000529"
     CDS             complement(3541..4566)
                     /locus_tag="ACHK07_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8100908.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4997..5218)
                     /locus_tag="ACHK07_000530"
     CDS             complement(4997..5218)
                     /locus_tag="ACHK07_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8100909.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5471..8575)
                     /gene="acrF"
                     /locus_tag="ACHK07_000531"
     CDS             complement(5471..8575)
                     /gene="acrF"
                     /locus_tag="ACHK07_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO8100910.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8587..9744)
                     /gene="acrE"
                     /locus_tag="ACHK07_000532"
     CDS             complement(8587..9744)
                     /gene="acrE"
                     /locus_tag="ACHK07_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO8100911.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10143..10805
                     /gene="acrS"
                     /locus_tag="ACHK07_000533"
     CDS             10143..10805
                     /gene="acrS"
                     /locus_tag="ACHK07_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO8100912.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10808..10987)
                     /gene="yhdU"
                     /locus_tag="ACHK07_000534"
     CDS             complement(10808..10987)
                     /gene="yhdU"
                     /locus_tag="ACHK07_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO8100913.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11071..11955)
                     /gene="yhdJ"
                     /locus_tag="ACHK07_000535"
     CDS             complement(11071..11955)
                     /gene="yhdJ"
                     /locus_tag="ACHK07_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8100914.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLSTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12041..12337)
                     /gene="fis"
                     /locus_tag="ACHK07_000536"
     CDS             complement(12041..12337)
                     /gene="fis"
                     /locus_tag="ACHK07_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO8100915.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12363..13328)
                     /gene="dusB"
                     /locus_tag="ACHK07_000537"
     CDS             complement(12363..13328)
                     /gene="dusB"
                     /locus_tag="ACHK07_000537"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO8100916.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13657..14538)
                     /gene="prmA"
                     /locus_tag="ACHK07_000538"
     CDS             complement(13657..14538)
                     /gene="prmA"
                     /locus_tag="ACHK07_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO8100917.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMTDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14550..16001)
                     /gene="panF"
                     /locus_tag="ACHK07_000539"
     CDS             complement(14550..16001)
                     /gene="panF"
                     /locus_tag="ACHK07_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO8100918.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15991..16233)
                     /gene="yhdT"
                     /locus_tag="ACHK07_000540"
     CDS             complement(15991..16233)
                     /gene="yhdT"
                     /locus_tag="ACHK07_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO8100919.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16342..17691)
                     /gene="accC"
                     /locus_tag="ACHK07_000541"
     CDS             complement(16342..17691)
                     /gene="accC"
                     /locus_tag="ACHK07_000541"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO8100920.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17702..18172)
                     /gene="accB"
                     /locus_tag="ACHK07_000542"
     CDS             complement(17702..18172)
                     /gene="accB"
                     /locus_tag="ACHK07_000542"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO8100921.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(19150..20124)
                     /gene="yhdH"
                     /locus_tag="ACHK07_000543"
     CDS             complement(19150..20124)
                     /gene="yhdH"
                     /locus_tag="ACHK07_000543"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO8100922.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20276..22216
                     /gene="csrD"
                     /locus_tag="ACHK07_000544"
     CDS             20276..22216
                     /gene="csrD"
                     /locus_tag="ACHK07_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO8100923.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22521..23564
                     /gene="mreB"
                     /locus_tag="ACHK07_000545"
     CDS             22521..23564
                     /gene="mreB"
                     /locus_tag="ACHK07_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO8100924.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23630..24733
                     /gene="mreC"
                     /locus_tag="ACHK07_000546"
     CDS             23630..24733
                     /gene="mreC"
                     /locus_tag="ACHK07_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO8100925.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24733..25221
                     /gene="mreD"
                     /locus_tag="ACHK07_000547"
     CDS             24733..25221
                     /gene="mreD"
                     /locus_tag="ACHK07_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO8100926.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25230..25823
                     /gene="yhdE"
                     /locus_tag="ACHK07_000548"
     CDS             25230..25823
                     /gene="yhdE"
                     /locus_tag="ACHK07_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO8100927.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25813..27282
                     /gene="rng"
                     /locus_tag="ACHK07_000549"
     CDS             25813..27282
                     /gene="rng"
                     /locus_tag="ACHK07_000549"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO8100928.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27350..31150
                     /gene="yhdP"
                     /locus_tag="ACHK07_000550"
     CDS             27350..31150
                     /gene="yhdP"
                     /locus_tag="ACHK07_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO8100929.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31489..32934
                     /gene="tldD"
                     /locus_tag="ACHK07_000551"
     CDS             31489..32934
                     /gene="tldD"
                     /locus_tag="ACHK07_000551"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO8100930.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGAMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(33062..33991)
                     /gene="aaeR"
                     /locus_tag="ACHK07_000552"
     CDS             complement(33062..33991)
                     /gene="aaeR"
                     /locus_tag="ACHK07_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO8100931.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            34174..34377
                     /gene="aaeX"
                     /locus_tag="ACHK07_000553"
     CDS             34174..34377
                     /gene="aaeX"
                     /locus_tag="ACHK07_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO8100932.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34385..35317
                     /gene="aaeA"
                     /locus_tag="ACHK07_000554"
     CDS             34385..35317
                     /gene="aaeA"
                     /locus_tag="ACHK07_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO8100933.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            35323..37290
                     /gene="aaeB"
                     /locus_tag="ACHK07_000555"
     CDS             35323..37290
                     /gene="aaeB"
                     /locus_tag="ACHK07_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO8100934.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPINEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            37382..37654
                     /gene="yhcO"
                     /locus_tag="ACHK07_000556"
     CDS             37382..37654
                     /gene="yhcO"
                     /locus_tag="ACHK07_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO8100935.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(37710..37973)
                     /gene="yhcN"
                     /locus_tag="ACHK07_000557"
     CDS             complement(37710..37973)
                     /gene="yhcN"
                     /locus_tag="ACHK07_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO8100936.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(38338..38808)
                     /gene="argR"
                     /locus_tag="ACHK07_000558"
     CDS             complement(38338..38808)
                     /gene="argR"
                     /locus_tag="ACHK07_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO8100937.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            39243..40181
                     /gene="mdh"
                     /locus_tag="ACHK07_000559"
     CDS             39243..40181
                     /gene="mdh"
                     /locus_tag="ACHK07_000559"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8100938.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(40244..41311)
                     /gene="degS"
                     /locus_tag="ACHK07_000560"
     CDS             complement(40244..41311)
                     /gene="degS"
                     /locus_tag="ACHK07_000560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO8100939.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(41401..42768)
                     /gene="degQ"
                     /locus_tag="ACHK07_000561"
     CDS             complement(41401..42768)
                     /gene="degQ"
                     /locus_tag="ACHK07_000561"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO8100940.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(42922..43320)
                     /gene="zapG"
                     /locus_tag="ACHK07_000562"
     CDS             complement(42922..43320)
                     /gene="zapG"
                     /locus_tag="ACHK07_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO8100941.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            43514..44641
                     /gene="zapE"
                     /locus_tag="ACHK07_000563"
     CDS             43514..44641
                     /gene="zapE"
                     /locus_tag="ACHK07_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO8100942.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHAVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            44860..45288
                     /gene="rplM"
                     /locus_tag="ACHK07_000564"
     CDS             44860..45288
                     /gene="rplM"
                     /locus_tag="ACHK07_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO8100943.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            45304..45696
                     /gene="rpsI"
                     /locus_tag="ACHK07_000565"
     CDS             45304..45696
                     /gene="rpsI"
                     /locus_tag="ACHK07_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO8100944.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            46091..46729
                     /gene="sspA"
                     /locus_tag="ACHK07_000566"
     CDS             46091..46729
                     /gene="sspA"
                     /locus_tag="ACHK07_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO8100945.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            46735..47232
                     /gene="sspB"
                     /locus_tag="ACHK07_000567"
     CDS             46735..47232
                     /gene="sspB"
                     /locus_tag="ACHK07_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO8100946.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47274..48641)
                     /gene="dcuC"
                     /locus_tag="ACHK07_000568"
     CDS             complement(47274..48641)
                     /gene="dcuC"
                     /locus_tag="ACHK07_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8100947.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            49021..49812
                     /gene="nanR"
                     /locus_tag="ACHK07_000569"
     CDS             49021..49812
                     /gene="nanR"
                     /locus_tag="ACHK07_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8100948.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49934..50827
                     /gene="nanA"
                     /locus_tag="ACHK07_000570"
     CDS             49934..50827
                     /gene="nanA"
                     /locus_tag="ACHK07_000570"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8100949.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            50936..52426
                     /gene="nanT"
                     /locus_tag="ACHK07_000571"
     CDS             50936..52426
                     /gene="nanT"
                     /locus_tag="ACHK07_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO8100950.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            52474..53163
                     /gene="nanE"
                     /locus_tag="ACHK07_000572"
     CDS             52474..53163
                     /gene="nanE"
                     /locus_tag="ACHK07_000572"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO8100951.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVEMLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            53160..54035
                     /gene="nanK"
                     /locus_tag="ACHK07_000573"
     CDS             53160..54035
                     /gene="nanK"
                     /locus_tag="ACHK07_000573"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8100952.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            54032..54496
                     /gene="nanQ"
                     /locus_tag="ACHK07_000574"
     CDS             54032..54496
                     /gene="nanQ"
                     /locus_tag="ACHK07_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO8100953.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(54556..55683)
                     /gene="yhcG"
                     /locus_tag="ACHK07_000575"
     CDS             complement(54556..55683)
                     /gene="yhcG"
                     /locus_tag="ACHK07_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO8100954.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSLYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(55867..57285)
                     /gene="gltD"
                     /locus_tag="ACHK07_000576"
     CDS             complement(55867..57285)
                     /gene="gltD"
                     /locus_tag="ACHK07_000576"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO8100955.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(57298..61758)
                     /gene="gltB"
                     /locus_tag="ACHK07_000577"
     CDS             complement(57298..61758)
                     /gene="gltB"
                     /locus_tag="ACHK07_000577"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO8100956.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIIETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSAFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            62433..63362
                     /gene="yhcC"
                     /locus_tag="ACHK07_000578"
     CDS             62433..63362
                     /gene="yhcC"
                     /locus_tag="ACHK07_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO8100957.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63458..65794
                     /gene="arcB"
                     /locus_tag="ACHK07_000579"
     CDS             63458..65794
                     /gene="arcB"
                     /locus_tag="ACHK07_000579"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO8100958.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            66024..66677
                     /gene="elbB"
                     /locus_tag="ACHK07_000580"
     CDS             66024..66677
                     /gene="elbB"
                     /locus_tag="ACHK07_000580"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO8100959.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            66674..67402
                     /gene="mtgA"
                     /locus_tag="ACHK07_000581"
     CDS             66674..67402
                     /gene="mtgA"
                     /locus_tag="ACHK07_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO8100960.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPERWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(67399..68031)
                     /gene="yrbL"
                     /locus_tag="ACHK07_000582"
     CDS             complement(67399..68031)
                     /gene="yrbL"
                     /locus_tag="ACHK07_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO8100961.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGERTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(68245..68517)
                     /gene="npr"
                     /locus_tag="ACHK07_000583"
     CDS             complement(68245..68517)
                     /gene="npr"
                     /locus_tag="ACHK07_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO8100962.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(68514..69368)
                     /gene="rapZ"
                     /locus_tag="ACHK07_000584"
     CDS             complement(68514..69368)
                     /gene="rapZ"
                     /locus_tag="ACHK07_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO8100963.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(69414..69905)
                     /gene="ptsN"
                     /locus_tag="ACHK07_000585"
     CDS             complement(69414..69905)
                     /gene="ptsN"
                     /locus_tag="ACHK07_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO8100964.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(70023..70310)
                     /gene="hpf"
                     /locus_tag="ACHK07_000586"
     CDS             complement(70023..70310)
                     /gene="hpf"
                     /locus_tag="ACHK07_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO8100965.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(70333..71766)
                     /gene="rpoN"
                     /locus_tag="ACHK07_000587"
     CDS             complement(70333..71766)
                     /gene="rpoN"
                     /locus_tag="ACHK07_000587"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO8100966.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(71814..72539)
                     /gene="lptB"
                     /locus_tag="ACHK07_000588"
     CDS             complement(71814..72539)
                     /gene="lptB"
                     /locus_tag="ACHK07_000588"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO8100967.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(72546..73103)
                     /gene="lptA"
                     /locus_tag="ACHK07_000589"
     CDS             complement(72546..73103)
                     /gene="lptA"
                     /locus_tag="ACHK07_000589"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO8100968.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(73072..73647)
                     /gene="lptC"
                     /locus_tag="ACHK07_000590"
     CDS             complement(73072..73647)
                     /gene="lptC"
                     /locus_tag="ACHK07_000590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO8100969.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(73644..74210)
                     /gene="kdsC"
                     /locus_tag="ACHK07_000591"
     CDS             complement(73644..74210)
                     /gene="kdsC"
                     /locus_tag="ACHK07_000591"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO8100970.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(74231..75217)
                     /gene="kdsD"
                     /locus_tag="ACHK07_000592"
     CDS             complement(74231..75217)
                     /gene="kdsD"
                     /locus_tag="ACHK07_000592"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO8100971.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(75231..76208)
                     /gene="yrbG"
                     /locus_tag="ACHK07_000593"
     CDS             complement(75231..76208)
                     /gene="yrbG"
                     /locus_tag="ACHK07_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO8100972.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
BASE COUNT        18428 a        19344 c        19934 g        18557 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa atttagcgtg
      121 ctgatatggc tcagttggta gagcgcaccc ttggtaaggg tgaggtcccc agttcgactc
      181 tgggtatcag caccactttt taggttaaag ttcggcagat tagaaaagaa tttgtctggc
      241 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
      301 gccgatggta gtgtggggac tccccatgcg agagtaggga actgccagac atcaaatcaa
      361 gcgaaaggcc atccgaaagg atggccttta tgcttttcga actaacattc aattaatgga
      421 ttacctgcga taaaaatgcc ctcgtacgct ctgacttagg atgcgcaaaa aactcatccg
      481 gtgcagcttg ctccactatt tcgccacgat ccataaaaat cacccggtca gcgacggttc
      541 gcgcaaaccc catctcatgg gttacacaca acattgtcat acccgattgc gccagcccaa
      601 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgatgtg ggctcatcaa
      661 acaacataat tttcggcttc atacacagcg agcgcgcaat ggcaacgcgt tgctgctgac
      721 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      781 aatgcatcgc cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca
      841 gggtacagtt ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc
      901 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta
      961 ccacgatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
     1021 taccggaacc tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
     1081 tatttttcaa aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg
     1141 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1201 ttaaaacgtt tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag
     1261 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1321 gcctgctgaa cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct
     1381 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1441 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1501 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1561 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1621 aacaaaggca gcatgaccga agacataaac agaacggtaa tcagcggcac gccgcgccag
     1681 aattcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac
     1741 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa ctgatgcaat aattaaagtt
     1801 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gagcaaaaaa accgccatac
     1861 atcagccacc agacaatcag tgggtaaatc actgcccaga ccgcaatata gcgaccgcga
     1921 tgcgggagta ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg
     1981 ttaattcgcc agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg
     2041 tggatgaaga cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccag
     2101 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     2161 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2221 atcgcgcggc ttgagttatg gctcgccggg cgcgagggat gagacagcaa tacttttgtc
     2281 atcatgactc cttagcgttc cacgatcgcg atgcggcggt tatagatatt catcagcagc
     2341 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2401 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2461 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggtggaata
     2521 atcacccgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc
     2581 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatccccgc gcggataatc
     2641 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2701 attaaaacca tcccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2761 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccga tgatcaacac tgcggcgatg
     2821 ggccaggtgc ggcgcagctg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2881 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2941 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     3001 acagcctggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     3061 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     3121 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3181 cacaatgcag aaaccagtag cgtattaagt aagccgacaa taaaaacgcg tccgtaggtg
     3241 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3301 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3361 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3421 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3481 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3541 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3601 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3661 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3721 gccttcttta cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3781 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3841 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3901 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcagggcata
     3961 cagttgcgac tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     4021 ttcgtcagag cgatcgaaag tcaccggtgt gtacttcata ttgtttgcct tgaaatagtc
     4081 ggcaacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     4141 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4201 gacgccagta aatgccattc ccatcccggc gtcgcgagat gaagtccagg tcgtattacg
     4261 ggagagcaaa tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg
     4321 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaatatc
     4381 aacatcaata cctgaaaatt taccgtcagc atcggcataa gagaacccag gtaatccatc
     4441 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4501 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4561 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4621 tacctgtctg tggccatcct cttataagca aaagtagtgc catgttttgc gcacgatttc
     4681 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4741 aaaatgaaac atacgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4801 taatgattgt tagcgtgagt atatttctgg tgggaagtgc gaaagttaaa ttgatgcgtt
     4861 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4921 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4981 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     5041 ctgctgctcc gccagcaata gccccagccg tttcccagtt atcctgagtt gtgcctttca
     5101 tacaggtatt ggtgtgaacc agacgcccct gatcgtcata aaccggaacg cagggcgaat
     5161 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5221 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5281 tgccatttta tcaccggagg ttataaatct tacggaatga ataatgacaa attatgtgaa
     5341 ttgtaaaaat agtgaaggga gcgggaacta tagaaagttt ttaaagagtg agtggaagaa
     5401 tatcttatta atttttatgg gtttcactgg aaataataaa ggcgcccgaa agcgccttta
     5461 tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacact ggtacgaaga
     5521 agattgccag caacgttgca gagaccattc cgcccattac cccgataccc actgcgttct
     5581 gcgcgccact gccggcaccg ttactgatag ccagcggtaa tacgccgaga ataaaggcga
     5641 gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc gcttcaacaa
     5701 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5761 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5821 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5881 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5941 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     6001 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     6061 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     6121 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6181 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6241 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6301 taacgtaagt cccacccagc gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6361 ctaatgcctg tgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc
     6421 cattaggacg cacgctgact aagctggcag gatgttgcgc cgccatacca agcaactggt
     6481 tacgggcctg ggttagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga
     6541 aacccgttgc cgtgcccagt tcaacaatgg ctggcatgtt gaatggaatg acaaaaccgt
     6601 cgcggatctt gcccaattcc attttggcac gatggattac cgcttccgca ctgttttcgt
     6661 caccattacg ctcttcccac ggtttcagac tgacgaaggc cataccggcg ttttgtgcct
     6721 ggccgctgaa gctaaagccg ttaaccgtaa agacactttc aacgttcgct ttctcgttct
     6781 tcagatagta atccgtcact tgatccaaca ctttttgcgt ccgctcttgc gtcgcgccag
     6841 cgggtaactg aatcatggtc agaaagacac cctgatcctc ttcaggtaag aaggaagacg
     6901 gaagacgtaa aaacaacact accattcctg caacaatcag cgcatagatc agtaaatatc
     6961 gtcctgtgga accgaggatt ttgccgacgc tgttggtgta gtggttaacg ctatgatcga
     7021 aggtggtatt aaaccagccg aagaaaccgc ccttattttc gtgatgctca gcagagacgg
     7081 gtttaagcag cgttgcacat aacgcagggg taagaatcaa tgccaccaga acagaaagcg
     7141 ccattgccga aacgatggtg atagagaact ggcgataaat tgccccagta gaaccgccga
     7201 agaatgccat cggaataaat accgctgaca gcaccatcgc gatacccacc agtgcgccct
     7261 gaatttgcga cattgatttt tccgtcgctt cttttggcgg gagcttatcc tccatcatca
     7321 cgcgctcgac gttctccacc accactatcg catcatcgac gagcagcccg atggcaagca
     7381 ccatcccgaa catcgttagt gtgttgatgg agtaaccaaa agcggcgagg atggcaaacg
     7441 tccctaacaa caccacgggt accgcaatgg tggggatcag cgttgctcgc atattctgca
     7501 agaacagata catcaccagg aacaccagca taatagcttc gaacagtgtt tttaccactt
     7561 cgtgaataga aagctggacg aatggcgtgg tgtcataagg gtagagaacc ttcattccct
     7621 gcgggaagaa tggctgtaat tccgccagtt ttgccttaat ggctttcgcg gtatcgagag
     7681 cgttcgcacc ggttgccagc ttaataccca ggcccgccgc cggttttccg ttgatgcgag
     7741 cgataacgtt atagttttca ccgccaagtt caacccgtgc gacatctttc agacgcacca
     7801 ccgagccgtc actgtttacg cgcagggtca ctttgccgaa ttcttccgga ttttttaacc
     7861 gcgtctgagc aataatcgag gcattcaact gttgccctgg taacgctggc gttccgccca
     7921 actgtccggc agcgatctga tcgttctgta ccttcaactg gttaatcaca tcaaccggtg
     7981 tcagtttata tttgtttagc agatcggcgt ccagccagat acgcatcgca tactgtgcgc
     8041 cgaaaagctg tacgtcaccg acgccattca gacggctaag cgtatcttta acgttagagg
     8101 ccacatagtc cgagatatcg tcctgtgtgg tgtctgggtt atcagagaca aagcccgcca
     8161 ccatcaaata gctgctactg gacttttcaa cactgatccc ctgctgctga acctcctgcg
     8221 gcagcaacgg cgtggcgagc tggagtttgt tctgcacctg cacttgcgcg atatcaggat
     8281 cggtcccgga ctggaaggta agggtaattg tcacgctacc ggcggaatcg ctggtggagg
     8341 acatatacat caggttatcg ataccgttca tattctgttc gataacctgc gtcaccgtat
     8401 cctgcacggt ctgcgcatca gcgcccggat agtttgctga aacagaaacc gcaggcggtg
     8461 caatggttgg atactgagcg acgggcaatt gtaggatcgc cagtgcgccc gccatcatca
     8521 gaataatggc cagcacccat gcaaatattg gtcgtcgaat aaaaaagttt gccatgtcag
     8581 attaccttac ttcgatgcag tatctgcggg ggtatcggta gtggctttaa cctgctctcc
     8641 cggacgcgct ttttgcaggc cgctgacaat gacttgatcg ccagatttca gtccttcact
     8701 aatcaaccat ttatcgccaa tcgcctgact ggcaacgaca gggcgcgctt caacctgact
     8761 tttatcgtta acaatcagca cggttgcatc accacgcggt gtgcggctaa cgccttgttg
     8821 cgggataaga atggcgtcag gttggacgcc ttcatcaatc cgtgcacgca caaacatacc
     8881 cggcaaaagc gtatgttgcg ggttagggaa gacagcacgt agggttatgg agccggtgct
     8941 ttcatcaacg gtcacatcgg agaattgcag cgtacctttc aggggatagg tttgaccgtt
     9001 ttccatgacc aactctacgt tgctggtggc gttttcctta tgcaaatttc cttgctctac
     9061 tgattgcttc agcctcataa aatcgttgct ggattgggtc acatcaacgt agataggatc
     9121 gagctgctgg acagtcgcca gttcagtcgt ttgcccatta gtgacaagag cgccttcggt
     9181 cacagtcgat ttgccgatac gtccgctaat tggcgcagtc actttggtat aagcaagatt
     9241 gatgcgagcg ctttcgactg tggcttttgc ggcaatcacg gcggcatcgg cctggcgagc
     9301 atcagcaatg gcctggtcgt actcctgctg gctgatgtat ttcgtaccca cgagcggaac
     9361 gtaacgtttt accgtcaaat gcgcgatggc ggcggcggct tcacttttcg ccagttcgcc
     9421 tttcgcgctg tcataatttg cctgataggt cgcgggatcg atctggtaca gggactggcc
     9481 tgcttgcaca tcgctgcctt cagtgaaatt gcgattcagt acgatcccgc taacctgtgg
     9541 gcgaacttcg gctatacgat aagcattggt gcggcctggt aattcagtct taacttctaa
     9601 cggggccgtt tttacaatat gaacggtaac ctgcggttca ccgacgtgag ctttctcttc
     9661 tcccttatcg ttacaaccgg cgattaaagc cgcggagatc agaataaagg agggcaggag
     9721 gaaaaacctg gcatgtttcg tcattactat tcctcaaaaa accaaaagcg cgttatttac
     9781 ccaaaaaggc aacgcgttaa ctcgcagaaa gaaaaataca gttcgctatc ctacaaatta
     9841 tcattcgtcg atgtaaggaa tagttatgaa tacaggcgtc tcaaggcaca taaacacaaa
     9901 aaaagattaa tattctactg ttttattttg acgcgggttg aaagaggcag aattaaaacc
     9961 tcgtaaattg aaatatatat tgatgtagtg aatgtatctt aggtaaataa tatatattat
    10021 ttatttaacg atttatagca actcaatatt agcctctcgt ataaatacac attaggtgat
    10081 agattaacct tcgctatttt ctcactctgt gtcgaatata tttatttcct gaataattaa
    10141 tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg gcaagaactg attgaaactg
    10201 ccatcgccca gtttgcgcag catggcgtaa gcaagacgac gctcaacgac attgccgacg
    10261 ccgctaacgt tacgcgtggc gctatctact ggcacttcga aaacaagact caactgttta
    10321 atgagatgtg gttgcaacag ccttcattgc gggagttaat ccaggaacac ttgacggctg
    10381 gattagagca tgacccgttt caacaattgc gtgaaaaatt gattgtcggc ttgcaatata
    10441 ttgccaaaat tccccgccag caggcgttgc tgaaaatctt atatcacaaa tgtgaattta
    10501 atgatgagat gctggccgag ggagtgatac gcgaaaagat gggctttaat ccgcagactc
    10561 tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt agcaaataac ctcgatttag
    10621 atgttgtgat gattattatt gatggtgcct tcagcggaat tgttcaaaac tggttaatga
    10681 atatggcggg ttatgatctt tataaacaag cccccgctct ggtcgataac gtattaagaa
    10741 tgttcatgcc agatgaaaac ataacgaaat taattcatca aacgaatgaa ttaagtgtca
    10801 tgtaaattca cattctgtcc tgaaaattca gttcgtcaca gttaaccagt tttagtggat
    10861 taactgagtt catattgcgg catttgtctg tttctgttgc cagcagttca atccactgca
    10921 tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac gacgagccac caatacttgc
    10981 gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg caggaaatat acacgaaatg
    11041 aataatctga aaaccaggct tacacttcct ttactttgca atgagatcgg ggtcaacttc
    11101 gcttaaccga cttcgttttg acaggttgcc ggtctttctt tttttcactt tcgccagttc
    11161 ttccgcagag taatgcgacg cgacatccag ccgtcgaagc cccattttga tgtactcgct
    11221 gttgatctca ataccaatga attttcgccc gctggagacg gctacggcac cggtagtaaa
    11281 gctaccggca aacgggtcga gaacgatatc gcctgggttg gaagaggcaa gaataatgcg
    11341 tttcagtaag gcttccggtt tttgcgtcgg gtggttctca tattcatcca tcaaataacg
    11401 cacgcgcgga aaatcccaga cgttacccgg tactttttga tgattgtaag gctgtggagg
    11461 atttttgcga taatcgatca acgcgcgctg cgatccagtt ttggcttcta ccagaatagc
    11521 atcaccgttg aacgtgtagt tctttgcgtc tttcaccatc atcaggatgg gttcgtacat
    11581 ggaaccgtag tgttttttcg cctgcactcc agaactgtca tatgaccaga cgatgcgact
    11641 tttgatggta aaaagcttgc ggcactggag atcgataaag ggcatgtttt ccgtgctgtt
    11701 catgatgtac atgctgccct gctttttcag aacgcggtgg cactctgcaa tcacttcaaa
    11761 cagccagtcg ataaacagat cttctttcca ggcttcgatc agaccatcaa aatttttacc
    11821 gatgttatac ggtgggtcgg caaagatcag atcgacgctt tcagtagata gctttttaag
    11881 ttcggcaagg gcatcaccgt gaataatggt cttagcttca ttaccaaacc gggtcggttc
    11941 acatcctgtt ctcatggtca ctccctttgt gacacctata aaaaaggcgc ttccccatgc
    12001 cgagtagcgc ctttttaatc aagcatttag ctaacctgaa ttagttcatg ccgtattttt
    12061 tcaatttttt acgcagcgta ccacggttga tgcccatcat cagtgcagca cgggtctggt
    12121 taccacgggt gtattgcatc accatgtcca acaggggctg ttctacttca gccagtacca
    12181 gctcatagag gtcattcaca tcctgaccat tcagttgagc aaaatagttc ttcagtgcct
    12241 gtttaaccga gtcacgcagg ggtttttggg ttacctgatc ctgagagtta acggtagaaa
    12301 cggtcagtac gtcagaattt acgcgttgtt cgaacatagt tctgtcagct ctttatttct
    12361 gtttacgcaa aattttcgaa gtatgcctcc aacgcctcca gctgttcgct ggcatcctca
    12421 atggcgttga atgtgcgccg aaactggtca tttggagcgt gttcctggag ataccaggaa
    12481 acgtgtttac gtgcaattcg gtaccctttt gccggaccat aaaagtcatg cagttcccga
    12541 acgtgcgcgc aaagcaagcg cttaacctct gccaaaggca gcgggggcag caactcccca
    12601 gtgtccagat aatgctggat ttcccgaaag atccagggtc ttccctgagc tgcgcggcct
    12661 atcatcaggg catccgcccc tgtatagtcg agcacagctc tggctttaag cgggtcagta
    12721 atgtcgccat tcgcgataac cggaatggaa actttctgct taactgcccg aatactgtcg
    12781 tactcagctt ctccattgaa caaacaggcg cgtgtacggc catgaatggt cagagcctga
    12841 atgccacagt cttcagccag ttgggcaatc tcttcgcagt tacggtgttc cggcgcccag
    12901 ccggtgcgaa tcttcagggt aacaggaacg tccactgcat tgacgacctc ggtaaggatc
    12961 gatttaacga catccgggta ctgcaagagg gctgaacctg cgagcttgcg attcactttt
    13021 ttagccgggc aacccatatt gatatcaata atctgggcac cgctttccac gttaatacgt
    13081 gctgcatctg ccatttcttt cggatcgcta ccagcaattt gcacggtgcg aataccgggt
    13141 tcatcaatgt gcaccatccg taaacgagat ttgtcgcttt cccaaacctg tgggttagaa
    13201 gacatcatct cggatactgt caatccggct cccatctcgt agcacaacgt ccgaaaaggt
    13261 ctgtctgtaa tgccagccat gggcgctgcg atcaggcgat ttctgagctg atattgtccg
    13321 atgcgcatga gttaagaaat gaccatactg tgactgcaag gcggcgtata ttacgcattt
    13381 tttgcacgag atgaaaggcc aaactttgac caatcctctg agatggatca aagaattgca
    13441 tttaaaatga gcgtggtgcg ataattactc ataaaaatca tcatattaga aaatagtgac
    13501 taaaatttac actcaaagaa atttgagtaa gttctcaatt tttctttatg aatgaaaatt
    13561 ttggcacgca aatttgcgta aataatcggc aaatttacgt gccttttgtg agcttgctcg
    13621 cacttcgccc cgcgtcaccc tacggcgatg cgaaggttaa ttcttacgac cggtaatacg
    13681 gcaccactct tctttttcca cgaccgggtc cagtgcgaag ctgtcggcat aagcttcaca
    13741 aacgctctct gcctggcttg ccagaatacc ggaaaggccc agcaaaccgc ctgaaaccgg
    13801 caggacgctg attaacggtg ccagttcacg taatgggcct gcaaggatgt tagcgaccac
    13861 cacgtcggct ttcatttctt ctggctgatc tttcggtaag tagagttcca gacggtcaga
    13921 aacgccatta cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat cgatatcaat
    13981 accaatggct tttgctgcac ccagtttcag cgccgcgatc gccagaatac cggaaccaca
    14041 gccaaagtcg atgactgttt taccggttaa atcgaggctg tcgagccatt gcaggcacag
    14101 agaggtggtt ggatgggtac ccgtaccaaa cgccagccct ggatctaaca tcacgttgac
    14161 ggcgttttcg tccggcacat cacgccagct tgggcagatc cacagtcgtt caccaaagcg
    14221 catcgggtgg aaattatcca tccattcgcg ctcccagtct ttatcttcca gttgttcgat
    14281 tttatgcgcg aagcctgcgc cgagcagcgg atggttttcc agaatcgcca ccacgtcggt
    14341 catatcggtt tcagcgtcga acagaccaat cacatcggtg tcgccccaca ggcgcgtttc
    14401 gcccggcagc ggctcaaata ctggcgtatc gtgggtgtcc tgaaaagtga tagaaacggc
    14461 acccgcttcc atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg tgttcagttt
    14521 cagttggatc caaggcatgg caaaactctt tatttatcag tagtcaaaac ggtagcttgc
    14581 gggacggatg taccgaaacg gtttccgacc aggaaagcca gcaaacttag tagtaacgag
    14641 ggcacgatag ggtggaagcc caggtactga atattcagcg tcgcgagtac ggcatacagc
    14701 acgccgccaa cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga
    14761 cccagcacca gcggccacag gaaaacggct tccagcccac cgaaggccag caaattcagc
    14821 cagatgatca tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc gagaactaac
    14881 gtaattaccg ccgacatccg cttcagacgc gtctcgtttt gcatttgatc cggacggata
    14941 ttcagataga gatctttaat gatcgtagcg gaactttgta gcaattgggc gttaattgtc
    15001 gacatgatcg cagccatagg tgcagccaga aagatcccgg cagcaaacgg tggcagcact
    15061 tttaccatta acgtcgggat caccagatcc ggtacggtga gatcggggat cactgcacga
    15121 cctaacgctc cggccaggtg cataccgaac atcaggattg cgactacaat cgtaccgatg
    15181 atgatccccc ggtgtactgc tttgctgtct ttataagaga tacagcgcac cgcagtatgc
    15241 ggcaggccta taacgccaaa gcacaccagt acccagaacg acgtcataaa ggcaggcgac
    15301 agaatatcgt cagcgccttg tggcgtaacc agttgcggat cgatggtttg caaggtctgt
    15361 actgcgttgc ttaagccgcc agcggcatgt accacgccaa taagcagcac aacggtgcca
    15421 atcagcatca caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa gccaccaaat
    15481 gcggtatata acgcaatgct gataccaaaa atcagcagcc cggtttcata aggaataccc
    15541 gccgcggttt ccagcaggcg cgcgccgccg ataaactgca cggtcatcgc gccaataaac
    15601 gcgaccagca aactcaaact cgccagccac accaaaagac gactctgata gcgggcaaac
    15661 agcatatcgt tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg
    15721 agaataccga gtgaaagcca gactgcagga agctgaatca tcgccagcaa tacccagcca
    15781 aggccgtatt tgtaagcagc tccaggcccg ccgataaacg aactggcact gatataagtc
    15841 gcggtgagcg tcatcgccag cacaataccg cccatagagc ggctgccgag gaaatactca
    15901 ttaaggaagg tgccggtgct ccgtttacgc atcgcataaa ccgagatacc gaacaccacc
    15961 accagatagg cgaccagcgg tagaattact tcaagctgca tcgtcatcct ccagcgggat
    16021 atcgcgatag ataaatttca ccatcgccca gcacagtcca ataaacagca gcggcgtcag
    16081 gatgcaggcc atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa
    16141 gtaagcggct gctaaccaaa ctgccagata caaaagggtc agccccagcg cccagcgcgc
    16201 ctctttatgg gcctgaacaa aacgagtgtc cattttttgt ccctgatggg tgaagaaagc
    16261 gaggattgta ccttatgggg gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg
    16321 ccttttgacg cttttgcagt cttatttttc ctgaagaccg agttttttct ccagatagtg
    16381 gatgttagtg ccaccatgct ggaagttctc gtcattcatg atgcggatct gcagatcaac
    16441 gttggttttg ataccgtcga tgatcagctc ctgcagcgca tttttcatgc gggcaatcgc
    16501 cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc attgagtcat agtacggcgg
    16561 tacggtgtag cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg
    16621 gaaacgggtg attttgcccg gacttggcag gaaggtgttc ggatcttcgg cgttgatacg
    16681 acattccacc gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc
    16741 ggcagcgata cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt ctgtaaccgg
    16801 gtgttctacc tgaatacggg tgttcatttc gatgaaatag aactcgccgt tttcgaacag
    16861 gaactcgaaa gtacctgcac cgcgatagcc gatatcaaca cacgctttag cgcaacgttc
    16921 gccgatgtag cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt cgaccacttt
    16981 ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga tagatagcgt tgccctgacc
    17041 gtcagccagt acctgaatct cgacgtggcg aggattttcc aggtatttct ccatgtaaac
    17101 catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg attgtgccag
    17161 ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccaccg ccgccgccgg aggctttgat
    17221 aatcaccgga taaccaatgc gtttagcaat ggcacggttt ttatccatat cgtcgcccag
    17281 cgggccgtca gaacccggta cgcaagggac gcccgctttt ttcatcgccg cgattgcgga
    17341 tactttgtcg cccatcaggc gaatggtttc tgctttcggg ccaatgaaga taaagccgga
    17401 gcgttcaacc tgctcggcaa agttggcgtt ctcggagagg aagccgtaac ccggatggat
    17461 tgctactgcg ccggtgattt cagcggcgct gatgattgcc gggatgttca gataactttt
    17521 tactgacggt gcagggccaa tacagaccgt ttcatctgcc agtaatacgt gttttagatc
    17581 gcgatccgcg ctggagtgca cagcgacagt cttgatgccc agttctttac aggcacgaag
    17641 aatacgcaat gcaatctcgc cgcggttggc aataacaatt ttatccagca tgttcgcctc
    17701 gttactcgat gacgaccagc ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa
    17761 ttgctttcac ggtaccggat ttgtccgctt cgatctggtt catcattttc atggcttcaa
    17821 cgatgcacag ggtatcgccc acgttgactt tctgacccac ttcgatgaac gcttttgcgt
    17881 ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg tgaccactga
    17941 tttccgctgc tgctggcgct tccatggaag gaacggtcgc cggagcggct gcgttagatt
    18001 gagctggctg ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg
    18061 caggagctgc acggctaatg cgtactgact cttcgccttc agaaatttcc agttcggaga
    18121 tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc atgagtgggt
    18181 tccgtactct ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg taaagcgtat
    18241 gccccgacag ctgtatgcat agcgataaat tccagcaggc cgggacgccg gtctattttg
    18301 ccccagagtg tcaatgttag acttgacgga cattgtgcag cgatctgctg ctatcctccg
    18361 gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg
    18421 cgtcagggag gacgaggtcg cacattataa cgatttcgta gcaattggca gctaaatact
    18481 ggtcttatca gggaagataa tcaacagcta acatgtaaat aaccttcaat accgtgtaat
    18541 ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat cgacggaact tcttataacg
    18601 taaggtgaaa agctgtaaag ccagccctgc gaagatgagg ggctgcaatg agataatctt
    18661 cacagaccac aaataatgta gggatgccag gatcgcgaca agatagacga cattacgcag
    18721 cggttgctgg ggattaccca attttcactg catcgtctgg atagatgtga atgctaaagc
    18781 acgcaaaatt aaccgattga caatgctcag cattaaatag gttcgagtga ttaatttgta
    18841 gtcaaacaaa atcagattat tcaccccatg gtaccagaaa taagtaaggg gttaagcgta
    18901 gcgtcgccca ggcaaagcac cataaaccta acaggcgacg gaggcgtatc aataatagct
    18961 gtttagtgga gtatcaatgc gcatcaattt atggtcagtc gaaatcatga ctttccagtt
    19021 tggatgatgt tgtacaactt catgaccaag gtgttctacg aaagctgtcc ggctaaccgc
    19081 ttttcccgtt aaccgattga ataatgattt caaaatgagt tccctctctt attattcctt
    19141 gctaaatggt tagttaacct tcaccagcgt gcgaccttgg atctggttat taatgatggc
    19201 ctcggcaaag ttcggtgcct ctgacagaga tatctctttt gccgcctggg tatagaatga
    19261 ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat
    19321 tactgaatcc accccttgca aacggacatt acgcagaata aatggcatga ccgtggttgg
    19381 cagagtaaaa ccacccgcca gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc
    19441 cagcactttt gccagcactt tgtcgccaac agtgtcaatt gccccagccc agacttgttt
    19501 ttccagagga cgggattcgg caaactcatc acgagggaga atacggctgg cacctaaact
    19561 tttcagatat tcatgggtac tttcgcgacc ggaaacggca acgacctgat aacccaactt
    19621 atgcagcagc gccacggcgg tactgccgac gccaccactg gcacccgtca cgacaatctc
    19681 cccgtcctgc gggcgaacac cggcatcttc cagcgccatc acacacagca tggcggtaaa
    19741 accggcagta ccgataatca ttgctttacg cgcgtccagc ccttgcggca tggcaaccag
    19801 ccagtcacct ttcactcgcg cctgctccgc cagcccaccc cagtggtttt caccaacgcc
    19861 ccagccagtg agtaacacct cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac
    19921 agttccggca aaatcgatcc caggaatcat cggaaaatta cggatgattt ttcccttacc
    19981 ggtaatcgcc agcgcatctt tatagttcag gctcgaccag tgaacatcga ccgtgacatc
    20041 gccctccggc aggcgacttt cgtccagagt ctgtactgat gcgagagttt tgccgtcctg
    20101 ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg catgatggat tggaaaataa
    20161 tttctgaaga ctatactcgc taatggaaaa gcaaagccga tgacgcgcaa taaattgcga
    20221 gataaatctg atttgctagt atgcccgctt cctcactatc ggagttaaca caaggatgag
    20281 attaacgacg aaattttcgg cctttgttac gctgctcacc gggttaacaa tttttgtgac
    20341 tttgctgggc tgttcgctaa gtttctacaa cgccattcag tataagttta gtcatcgcgt
    20401 tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg aatgacttca gcgcattaag
    20461 gccacaaatt accgaattaa tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg
    20521 tgataaacag gtttataccc tggccagaaa tggtagttat cgtccggttg gcaccagcga
    20581 tctgtttcgt gaactgagcg ttccgttgat aaagcatccg gggatgtcgt tgcgtctggt
    20641 ttatcaggat ccgatgggca actatttcca ttcgttgatg accaccgcgc cgctcacggg
    20701 ggcgattggc tttatcattc ttatgctctt tctggcggta cgctggttac aacggcaact
    20761 tgccgggcaa gaattgctgg aaacccgggc tactcgtatc ttaaacggtg agcgtggctc
    20821 taatgtgttg ggaaccatct atgaatggcc gcccacaacc agcagtgcgc tggatacgct
    20881 gcttcgtgaa attcagaacg cacgcgaaca acacagccgt cttgatacgc tgatccgctc
    20941 ttatgccgca caggacgtga aaaccggcct caataaccga ctctttttcg ataatcagtt
    21001 agcaacgtta ctggaagatc aggagaaagt aggtacccac gggatcgtga tgatgattcg
    21061 tctgccggat ttcaatatgt tgagcgatac ctgggggcac agccaggttg aagaacagtt
    21121 cttcactctg acgaatctgc tgtcgacatt tatgatgcgc taccctggcg cactgctggc
    21181 gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa aagaggcaga
    21241 gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg ccgaacaata aaatgctcga
    21301 tcgcgacgat atgatccaca ttggtatctg cgcctggcgt agtggtcagg ataccgagca
    21361 ggtaatggaa catgcagagt ctgccacgcg taatgcggga ttgcagggcg gcaatagctg
    21421 ggctatttac gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct
    21481 tatcgagcaa atgctcagtc gcggcggccc gcgcctttat caaaaaccgg cggttactcg
    21541 cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc tttgatggta atgaagaggt
    21601 tagctcggca gagtatatgc cgatggtctt gcagtttggc ttatcggaag agtatgaccg
    21661 tctgcaaatc agccgtctta ttccactatt gcgttactgg ccagaggaaa atctggcgat
    21721 tcaggttacc gttgagtcgc tgattcgccc gcgttttcag cgttggctgc gcgatacgtt
    21781 aatgcaatgt gaaaaatcac aacgaaaacg cataattatt gaacttgcag aggccgatgt
    21841 aggtcaacat atcagtcgtt tacaacctgt tattcgttta gtgaatgctt taggggtacg
    21901 ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc agttggatca aagaacttaa
    21961 tgttgagtta ctcaagctcc atccggggct ggtcagaaac attgagaagc gaacggagaa
    22021 ccagctgctg gttcaaagcc tggtggaagc ctgctcaggg accagcaccc aggtttacgc
    22081 caccggtgtg cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg
    22141 gcaaggggat tttttcgcgt cctcacagcc acttgatact aacgtgaaaa aatattcaca
    22201 aagatactcg gtttaacctg ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt
    22261 ctcatgggag tgtgcttgtc tgctcgccag attgttgcag cacatatgca gatgaatgac
    22321 cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt ctggactcag
    22381 gcagagattt gtaacaaagg aaacgaactg cactaatttt caccgtagca gatgattttt
    22441 gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg attttctttt ccgccccagc
    22501 tttcaggatt atcccttagt atgttgaaaa aatttcgtgg catgttttcc aatgacttgt
    22561 ccattgacct gggtactgcg aataccctca tttatgtaaa aggacaaggc atcgtattga
    22621 atgagccttc cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg
    22681 cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt gctgccattc
    22741 gcccaatgaa agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg ctccagcact
    22801 tcatcaaaca agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg
    22861 taccggttgg cgcgacacag gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg
    22921 gtgcccgtga agtcttcctg attgaagaac cgatggctgc cgcaattggt gctggcctgc
    22981 cggtttctga agcgaccggt tctatggtgg ttgatatcgg tggtggtacc actgaagttg
    23041 ctgttatctc cttgaacggc gtggtttact cctcttccgt acgcattggt ggtgaccgtt
    23101 tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca
    23161 ccgcagaacg tatcaagcac gaaatcggtt cggcttatcc gggcgatgaa gtccgtgaaa
    23221 tcgaagttcg tggccgtaac ctggcagaag gtgttccacg cggttttacc ctgaactcca
    23281 acgaaatcct cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg
    23341 cactggaaca gtgcccgccg gaactggctt ccgacatctc cgagcgcggc atggtactca
    23401 ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt aatggaagaa accggcattc
    23461 cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg
    23521 aaatgatcga catgcacggc ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg
    23581 gaagcgtctg tttaccctgc ctggtctgat acgagaatac gcataactta tgaagccaat
    23641 ttttagccgt ggcccgtcgc tacagattcg ccttattctg gcggtgctgg tggcgctcgg
    23701 cattattatt gccgacagcc gcctggggac gttcagtcaa atccgtactt atatggatac
    23761 cgccgtcagt cctttctact ttgtttccaa tgctcctcgt gaattgctgg atggcgtatc
    23821 gcagacgctg gcctcgcgtg accaactaga acttgaaaac cgggcgttac gtcaggaact
    23881 gttgctgaaa aacagtgaac tgctgatgct tggacaatac aaacaggaga acgcgcgtct
    23941 gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag aaaatggtga ctcaggttat
    24001 ctccacggtt aacgatcctt atagcgatca agttgttatc gataaaggta gcgttaatgg
    24061 cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt gttggtcagg tggtggccgt
    24121 cgctaaactg accagtcgcg tgctgctgat ctgtgatgcg acccacgcgc tgccaatcca
    24181 ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac ggttgtacgg atgatttgca
    24241 gcttgagcat ctgccagcga atacggatat tcgtgttggt gatgtgctgg tgacctccgg
    24301 tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg taaaactcga
    24361 tacccagcgc gcttatactg tgattcaggc gcgtccgact gcaggactgc aacgtttgcg
    24421 ttatctgctg ctgctgtggg gggcagatcg taacggcgct aacccgatga cgccggaaga
    24481 ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc
    24541 agacgcgatg gggccaaagt tacctgaacc ggcaacgggg atcgctcagc cgactcctca
    24601 gcaacccacg acaggaaatg cagctactgc gcctgctgcg ccgacacagc ctgctgctaa
    24661 ccgctctcca caaagggcta cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc
    24721 gggagggcaa tagtggcgag ctatcgtagc cagggacgct gggtaatctg gctctctttc
    24781 ctcattgcgc tgttgctgca aatcatgccc tggccggaca acctgattgt tttccggcca
    24841 aactgggtgt tactcatctt gttgtattgg atcctggcct tgccccatcg cgtaaatgtg
    24901 ggcacaggtt ttgtgatggg tgccatactg gatctgatca gcggctcgac gcttggcgta
    24961 cgcgtattgg cgatgagcat cattgcttac ctggtggcgc tgaaatacca gcttttccgc
    25021 aacctcgcat tatggcagca ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt
    25081 attgttttct gggcagagtt tttagtgatt aacgtctctt tcagaccgga agtgttctgg
    25141 agtagtgtag tcaatggggt gctctggccg tggattttct tgctgatgcg taaagtccgt
    25201 cagcagtttg cagtgcaata aaggtttcta tgacttctct gtatttagct tccggttctc
    25261 cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca
    25321 ttgaggagca gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt ctggcgcgcg
    25381 agaaagcacg ggcaggtgtc gcgcaaacgg cgaaggatct cccggtactg ggtgcggata
    25441 ctatcgttat cctgaacgga gaagtgctgg agaaaccgcg cgacgcagag catgcggcgc
    25501 agatgttgcg caaattatcg ggtcagaccc atcaggtgat gacggcagtg gcgttggccg
    25561 acagccagca cattctcgat tgcctggtgg tcaccgatgt gactttcaga acgttaacag
    25621 acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg
    25681 gtattcaggg gctgggtggc tgttttgtca ggaagataaa tggcagctat cacgccgtag
    25741 tcggcttacc gctggttgaa acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga
    25801 aaagggataa acatgacggc tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg
    25861 gcgtatattg atggcggtat tctgcaggaa attcatattg aacgtgaggc gcgacgcgga
    25921 atagtaggca atatctacaa gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct
    25981 tttgtagata ttgggctgga taaagccgcg tttcttcatg catctgacat catgccgcac
    26041 accgaatgtg tggcgggtga agaacaaaag caattcacgg tgcgcgacat ctcggaactg
    26101 gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag atccgcttgg cactaagggg
    26161 gcgcgcctga ccaccgatat cacgctgcct tctcgctatc tggtctttat gccaggggct
    26221 tctcacgttg gtgtttccca acgtattgaa agcgaatcag aacgtgaacg cctgaaaaaa
    26281 gtggtcgcgg agtattgcga cgagcagggc gggtttatca tccgtactgc agcggaaggg
    26341 gttggcgagg ctgaactggc ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt
    26401 atggagcgta aaaagcgccc gcagacccgt tatcagctgt acggcgaact ggcgctggcg
    26461 cagcgtgttc tgcgtgattt cgccgatgcc gaactggacc gcattcgcgt tgactcacgc
    26521 ctgacttacg aagcattact tgagttcacc tcggagtaca ttcccgagat gaccagcaag
    26581 ctggagcatt acaccggacg ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc
    26641 cagcgagcgc tggaacgcaa agtagaactg aaatccggtg gttatctcat tatcgaccag
    26701 accgaagcga tgaccaccgt ggacatcaat accggggcgt ttgtcggtca tcgcaatctg
    26761 gacgacacca ttttcaatac caatattgaa gcgacgcagg ctatcgctcg ccagttacgg
    26821 ttgcgcaatc tgggcgggat tatcattatt gatttcatcg atatgaataa tgaagatcac
    26881 cgccgccgcg tgctgcattc gctggagcaa gcgttgagca aagaccgggt gaaaaccagc
    26941 gttaatggtt tttcggcgct ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc
    27001 attgagcacg tactgtgtaa cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc
    27061 gtggaaacgg tatgctatga aatcatgcgc gagattgttc gtgtccacca tgcttacgac
    27121 tccgaccgtt tcctggtcta tgcttctccg gcagtagctg aagccttgaa aggcgaagag
    27181 tcacactcgc tggcggaagt ggaaattttc gttggtaaac aggttaaagt acaaattgaa
    27241 ccgctctata accaggagca gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca
    27301 tccggcaaag ggtttttgag tcacatattt agcagacaag gagtgacggg tgaggcgatt
    27361 gccggggatt ttactgctta ctggagccgc gctcgttgtg atcgctgccc tgctggttag
    27421 cggcctgcgt attgctttac cgcatcttga cgcctggcgt ccggaaatcc tcaacaaaat
    27481 agaatccgcg actggcatgc cggttgaagc cagtcagctc tcagccagct ggcagaattt
    27541 tggcccgacg cttgaagcac acgacatccg tgcagaacta aaagatggcg gcgaattttc
    27601 ggttaaacgc gttactctgg cgctggatgt ctggcagagc ctgttacata tgcgctggca
    27661 gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc aacactccta tcaccagcgg
    27721 tggtagcgat gacagtctgg aagccagtca catcagcgat ctgtttcttc gtcaatttga
    27781 ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg ccatccggtc agcgcgccga
    27841 gctggcgatc ccacaactca cctggctgaa cgatccacgt cgacaccgtg cggaaggcct
    27901 ggtaagcctc tccagcctta ccggacagca cggcgtgatg caggtgcgca tggatttgcg
    27961 cgatgatgag gggttgttaa gcaatggtcg cgtctggctc caggcggatg acatcgacct
    28021 gaagccgtgg ctcggtaaat ggatgcagga caatattgcg ctggaaacgg cacagttctc
    28081 ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa
    28141 acagggcggt gccagctggt tgggcgagaa gcaaacgcat acgctgtcgg tggataatct
    28201 gaccgcgcat attacgcgtg aaaatccggg ctggcagttc tctattcccg atacacggat
    28261 cacgatggac ggcaaaccct ggccgagcgg agcattgacg ctggcctgga taccggaaca
    28321 ggacgttggc ggcaaagaca ataaacgcag tgacgaactc cggattcgcg ccagtaatct
    28381 ggagctggca ggcctggagg gcatacgccc gctggccgcg aaactttcac ctgcactggg
    28441 tgatgtttgg cgctccacac aaccgagcgg caagattaac acgctggcgc tggatatccc
    28501 gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg agcgatctgg cctggaagca
    28561 atggaaatta ttaccgggtg cggaacactt ctccgggacg ctttccggca gcgttgaaaa
    28621 tggtttgctt accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc
    28681 gccactggaa atcgccgacg gccaggcaac tataagctgg ctgaacaatg acaaaggttt
    28741 ccagctggat gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt
    28801 tcgttacctg caacctgcta acgatgaacc ctggctgggt attctggctg gcatcagtac
    28861 cgatgatggt tcacaagcct ggcgctattt cccggaaaac ttgatgggta aagacctggt
    28921 tgattactta agtggcgcga ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg
    28981 tggcaatccg caactcttcc cctataaaca caacgaaggt cagtttgaag tgctggtgcc
    29041 gctgcgcaac gcgaagtttg ccttccagcc ggactggcct gcattaacta accttgatat
    29101 tgaactggac tttattaacg acggtttatg gatgaaaacc gatggcgtta atctgggcgg
    29161 cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac tcgaaagaaa aactgctgat
    29221 tgacgctgac attaaaggtc cgggtaaagc cgttggccct tactttgatg agacaccgct
    29281 gaaagattct ctgggtgcga ccctgcaaga actccagctc gacggcgatg tgaatgctcg
    29341 cttacatctt gatatcccgc tgaacggcga actggtaacc gcgaaaggtg atgtgacgct
    29401 gcgtaataac agtctgttta tcaaaccact cgacagcacc ctgaaaaatt tgagcggtaa
    29461 attcagcttt atcaatggcg atctgcaaag tgaaccactg acagcaagct ggtttaatca
    29521 gccgttgaac gtggattttt ccaccaaaga aggggcaaaa gcctaccagg tcgcggtgaa
    29581 tctcaacggt aactggcaac cagcgaaaac cggcgttctg cctgcagcgg tgaacgaagc
    29641 attgagtggc agcgtggcgt gggatggtaa agtgggcatt gatctgcctt atcatgctgg
    29701 cgcgacctat aacgtagagc tgaacggcga tctgaagaat gtgagcagtc acttaccttc
    29761 accgttagcc aaacctgcgg gtgaaccact agcggtaaac gttaaggttg atggcaatct
    29821 caacagcttt gaattaaccg gacaggctgg tgcggataac catttcaata gccgctggtt
    29881 gctcggtcaa aagctgacgc tcgatcgtgc tatttgggcg gcagacagta aaacgctccc
    29941 gccgttgccg gaacaaagtg gtgttgaact caatatgccg ccgatgaatg gtgccgagtg
    30001 gctggccctg tttcagaaag gtgcggcgga gagtgtcggt ggtgcagcga gtttcccaca
    30061 acacataacg ttacgtacgc ctatgttgtc gctgggaaat cagcaatgga ataacctgag
    30121 tattgtttcg caaccgacgg caaatggcac ccaggttgag gcgcaagggc gtgaaatcaa
    30181 cgccacgctg gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat atctttatta
    30241 caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac
    30301 ggagcgcatt aacttccgtg gctggccgga cgcacaaata cgatgcgcag agtgctggtt
    30361 ctgggggcaa aaattcggtc gtattgacag tgatctcacc atttctggcg atacattaac
    30421 gctgaccaat ggactgattg ataccggttt ctcgcggctt actgccgatg gtgaatgggt
    30481 taataatccg gggaatgaac gtacctcgct gaaaggaaaa ctgcgcgggc agaaaattga
    30541 tgccgccgca gaattttttg gtgtcacgac gcccatacgg cagtcgtcat ttaatgtgga
    30601 ttacgattta cactggcgta aagcaccgtg gcagccagat gaggcgacgt tgaatggcat
    30661 cattcatact caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca
    30721 attgctgcgc ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga
    30781 cacttttggc gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg
    30841 cgttatgcac accgacgaca cgttggtgga tggcctggag gcggatatcg ccatgaaagg
    30901 gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc
    30961 tgcgacggtg ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt
    31021 tgctgccagt aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat
    31081 ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa
    31141 agcgcaatga tttgacgagg gcgcgtaatt gccccaatct cataagataa tcgttgccaa
    31201 aggccaacga gccagaacat aaccgtaggc cggatagggc gtttacgccg catccggcag
    31261 ccgttgcctg atgcgacgct gtgcgtctta tcaggcctac aaacggaaca taaccgtagg
    31321 tcggataagg cgtttacgcc gcatccgaca gccgttgcct gatgcgacgc tgtgcgtctt
    31381 atcaggccta caaaccggaa cataatcgta ggccggataa ggcgtttacg ccgcatccgg
    31441 cagccgtaaa aaatcctcta ctgcagtaac taacgagtag caaaaacgat gagtctgaac
    31501 ctggtaagtg aacaattgct agcggcgaac ggcctgaaac atcaggactt gttcgcgatc
    31561 ctcggtcaac tggccgaacg tcgccttgat tatggcgatc tctattttca gtcgagctat
    31621 cacgaatcct gggttttaga agaccgcatt attaaagatg gttcttacaa catcgatcag
    31681 ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat ttgcttacgc tgaccaaatc
    31741 agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca ccatcgtccg tgatagtggt
    31801 gatggcaaag tacagacgct gggcgcggta gagcatagcc cgttgtatac ctcggtagat
    31861 ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc tgcgtcgcgt cgataaggtt
    31921 gcccgcgaag cggacaagcg cgtacaggaa gtgactgcca gcctcagtgg tgtctatgaa
    31981 ttaattttgg ttgcggccac cgacggcacg ctagcggcgg atgtccgtcc gctggtgcgt
    32041 ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg aacgcggtgc cagtggcggc
    32101 ggcggtcgtt ttggttatga gttcttcctt gccgatctcg acggcgaagt ccgtgcggat
    32161 gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc tttctgccgt tgctgcacca
    32221 gcgggcacca tgccggtagt acttggcgca ggttggccgg gcgtgctgtt gcatgaagcg
    32281 gttggtcacg gtctggaagg cgacttcaac cgccgtggca cttcagtatt tagtggacag
    32341 gtcggggagc tggtggcttc agaactgtgt accgtggttg atgacggcgc gatggtcgat
    32401 cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag gccagtacaa cgtgctgatt
    32461 gagaacggca ttctgaaagg ctacatgcag gataaactca acgcgcgttt gatggggatg
    32521 acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc tgcccatgcc gcgtatgacc
    32581 aacacctata tgctgccggg taaatcgacc ccgcaggaaa ttattgaatc cgttgagtac
    32641 ggtatctatg caccgaactt tggtggcggt caggtggata tcacctccgg caaattcgtt
    32701 ttctccactt cagaagcata tctgattgaa aacggtaaag taacgaagcc ggtgaaaggc
    32761 gcaacgttga ttggttccgg tatcgaaacc atgcagcaga tttcgatggt tggcaacgac
    32821 ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag ggcaaagttt gccggttggc
    32881 gtgggccagc caacgttgaa agtcgataac ctgactgttg gcggtactgc gtaataattt
    32941 cagtattttc agaaggataa ttaatctttc tacgtgcacg aacggtcccc tcgctcctct
    33001 ggggttaggg tgaggggaac ccgttggcac aggtttgtac aacgtaacag tacaagatga
    33061 attacttctc tttcccgcgc ccgtgcatct cctgaaacaa tttaccgacc tcaacaaaat
    33121 aatccgtcag cgagttgatc acgacctgta ccttcagcgg cagcttatct ttttcggtat
    33181 ataacgcata aaccgggcgt ggatctgact gataacgcgg cagcaggatc tccagctccc
    33241 cacgattgat ctcgttgatc acccacatca gcggcacgta ggcgatcccg gcacccgccg
    33301 ttagccagcg caccagcgtc atcggatcat tagtcacaaa tctcccttgc gggatcaggc
    33361 gagtcgagat cccttccggt gcaatcagtt caaattcatt gtcgggccgc acgctgtatt
    33421 caagccatga atggctactt aaatcggcgg gtttttccgg tatgccgtat tgtgtgagat
    33481 agcttttcgc ggcgcacacc accattggca tcgcgccaag acggcgggaa aacaggctgg
    33541 aatcctgcaa cgcgccgacg cggatcacca catccagacc gtcggcaatc aggtcggggg
    33601 ctggaattcc ggtaaccaga ttgacgctca aacctgggta ttctttcagc attttggctg
    33661 tcagcccggc gagaacattt tgtgccatag ttgaagaaca gccaatgcgt agcgtcccga
    33721 tgggggtgtt attgaaggca tacagttgct catgaacatc ctgcacttca tgaagcatac
    33781 gacggcagcc ctggtagtaa attctaccgg cttcggtcag gccaatgctg cgtgtgctac
    33841 ggtttaacag ctttacctgc aactcatctt ccagttttga caccgtctga ctgatggacg
    33901 aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga gccaaattca actactttgg
    33961 caaacaccga catgcgtttt agtcgttcca ttattcactc tgacttaaaa gtgatttaga
    34021 tcacataata tagataacag cataacagtt acgctaatat attaaatatc aatctacagc
    34081 aatgttgctc tcgcccggct ttccatgccc ttctctgcgg cgacagatgc tgaaaataat
    34141 aacgcctgct ctctctatac aaccaaggtc aacatgagtc tgtttcccgt tatcgtggtg
    34201 tttgggctgt ccttcccacc gatatttttt gaattgcttt tatcactggc gattttctgg
    34261 ctggtgcgcc gggtacttgt gccaacaggt atctacgact ttgtctggca tccggcgttg
    34321 ttcaacaccg cgctctattg ctgcttgttt tatttgatat cgcgactgtt cgtttgaggt
    34381 tgaagtgaaa acactaataa gaaaattctc ccgtacggcc atcacggtcg tattagtcat
    34441 tctggccttc atcgcaattt ttaatgcctg ggtctattac accgaatccc cgtggacgcg
    34501 tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg gacgtttctg gactcattac
    34561 ccaggtgaat gttcatgata accagctggt gaaaaaagga caggtactgt tcaccatcga
    34621 ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt attatcaggt
    34681 actggcacag gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg tgcaggcgat
    34741 gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa acggttctgc atcagttagc
    34801 gaaagcgcaa gcgacccgcg atctggcaaa actggatctt gaacgcacgg tgatccgcgc
    34861 gccagcggat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta ttactcgtgg
    34921 atcaacggcg gttgcgctgg tgaaacagaa ctctttctat gtactggcct atatggaaga
    34981 aactaagctg gaaggggtgc gccctgggta tcgtgcagag atcacgccgc ttggcagtaa
    35041 caaagtgctg aaagggactg ttgatagtgt tgccgctggg gtcaccaacg ccagcagcac
    35101 gcgtgacgac aaagggatgg cgaccatcga ctctaacctt gaatgggtgc gtttggcgca
    35161 acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc ctgcgggcac
    35221 cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg attcgttctt
    35281 ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat tttctccatt
    35341 gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact ggcgctgttt
    35401 gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc ggcgattgtt
    35461 gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat tcgctatcgt
    35521 ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt gatcatcatt
    35581 gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc cggtttttgt
    35641 acctggatat cctcgctggt acgaatagaa aactcgtatg cgtgggggct ggccggttat
    35701 accgcgctga tcattgtgat caccattcag ccggaaccat tgcttacgcc gcagtttgcc
    35761 gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg cgattatggc ggatttgctc
    35821 ttttctccgc gatcgatcaa acaagaagtg gatcgagagc tggaaagttt gctggtcgcg
    35881 caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt cgataaagcc
    35941 tggggcgacc tggttcgacg caccacggcg ctacaaggca tgcgcagcaa cctgaatatg
    36001 gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac gctatcgctg
    36061 acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc ggaattgatc
    36121 actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga cgtccacaag
    36181 cagctcaaac gcctgcgaag agttatcgcc tggaccgggg aacgggaaac gcctgtcacc
    36241 atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg cgttatcagt
    36301 aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga agtaaaagta
    36361 gagtcagccg aacgtcatca tgcaatggtt aacttctggc gaaccacact ttcctgcatt
    36421 ctgggcacgc ttttctggct gtggacgggc tggacttccg gtagtggtgc aatggtgatg
    36481 attgcggtag tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt ggcgatcgac
    36541 tttatctacg ggacgctggc cgcgctgccg ttagggctgc tctacttttt ggtgattatc
    36601 cctaataccc aacagagcat gttgctgctg tgcattagcc tggcagtgct gggattcttc
    36661 ctcggtatag aagtacagaa acggcgactg ggctcgatgg gggcactggc cagcaccata
    36721 aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct cgacagcgca
    36781 ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt gcgggataaa
    36841 tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc tgtttccgcg
    36901 atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta tcagcagctg
    36961 tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc gctgacgatg
    37021 attatcgcgc accagcgcct gcgtgatgcg ccgatcccga ttaacgagga tttatcggcg
    37081 tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag cgatgataaa
    37141 cgtcgtcggt actttggtca gttgctggaa gaactggaaa tctaccagga aaagctacgc
    37201 atctggcaag ctccaccgca ggtgacggaa ccggttcatc ggctggcggg gatgctccat
    37261 aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag cgtcggtttt
    37321 ttcatggcta tacttagcga tgaacggcag aactcgccgc gaaacgtgac ggtggcaaca
    37381 gatgaatatt tatacctttg attttgatga gattgagagt caggaggatt tttatcgcga
    37441 cttcagccaa gcctttggtc tggcgaaaga taaggtacgc gatctcgact cactatggga
    37501 tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc atctgggaga
    37561 gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag aggaagagct
    37621 ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc cgaaccgggg
    37681 gcaatatcgt cggacaagac gatgagggtt tatttgtaca gttcagccgt agcgtgccag
    37741 gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc ttccgctttt
    37801 ttgttcagca tttcacgcat atccattggc gaagacgcca caccacttac ggatacggtc
    37861 ccgattgctt cacgattttg tgcttgtgca gcatcaatgg agtcggcagc gaatgcaccg
    37921 aaagagagaa cagaaagtac gcttaatgca gcaacagtgg ttttgatttt catgattttt
    37981 acctcgacat aatcttttag ctgggccttt gtttcgtgac ccatatcaca aaatcaagta
    38041 tacactaatt attggactaa ttaataccac gctaactgtt tctgttgata ctaagtgtta
    38101 aaaatgtgaa ttttaataac aaaaaagaat tgaaaatacg gttatttatc gttaattcta
    38161 gtattttcaa ttagataatg aattttgcat aatgtgcttt ttttaccgta tgtattcatt
    38221 gtgtgaatga catgtcgcag taaaacgcac tattcgttaa attaatcgca ggggaaagca
    38281 gggggttgag agggataagc aacattttcc ccgccgccag aagcgacggg gcagagatta
    38341 aagctcctgg tcgaacagct ctaaaatcgc ttcgtacagg tctttgacgg tgaaaccgtt
    38401 agcaggggtg gtaaagatgg tgtcatcgcc agcgatggtg cccagaatac cttctgcttt
    38461 gcccagtgag tccagcaggc gagcaattaa ctgcgccgcg ccagggctgg tatgaatcac
    38521 gacaactgca tcgttgtagt cgatatccag caccagattt ttcaatggac tggaggtggt
    38581 tggtacaccc agttcagctg gcaggcagta aaccatttcc attttggcat tgcgtgtacg
    38641 tacagcacca aacttggtca acatccgcga gactttagac tgattaatat tgtcaaagcc
    38701 ttgctcctgc aacgcggcga cgatttcgcc ttgggagcta aatttctctt ctttaagtaa
    38761 tgctttaaat gctttaacta gttcttcttg cttagccgag cttcgcataa gtcacccgat
    38821 atggtggttg atacaacatt attgtgcata cagatgaatt tttatgcaaa cagtcagccc
    38881 tgaagaaggc tgaaataatg ttatgaaaga gcgggatttt atcaaatttc gttattgaga
    38941 aacatgcctg cgtcacggca tgcaaattct gcttaaaagt aaattaattg ttatcaaatt
    39001 gatgttgttt tggctgaacg gtagggtata ttgtcaccac ctgttggaat gttgcgctaa
    39061 tgcataagcg actgttaatt acgtaagtta ggttcctgat tacggcaatt aaatgcataa
    39121 acgctaaact tgcgtgacta cacattcttg agatgtggtc attgtaaacg gcaattttgt
    39181 ggattaaggt cgcggcagcg gagcaacata tcttagttta tcaatataat aaggagttta
    39241 ggatgaaagt cgcagtcctc ggcgctgctg gcggtattgg ccaggcgctt gcactactgt
    39301 taaaaaccca actgccttca ggttcagaac tctctctgta tgatatcgct ccagtgactc
    39361 ccggtgtggc tgtcgatctg agccatatcc ctactgctgt gaaaatcaaa ggtttttctg
    39421 gtgaagatgc gactccggcg ctggaaggcg cagatgtcgt tcttatctct gcaggtgtag
    39481 cgcgtaaacc gggtatggat cgttccgacc tgtttaacgt taacgccggc atcgtgaaaa
    39541 acctggtaca gcaagttgcg aaaacctgcc cgaaagcgtg cattggtatt atcactaacc
    39601 cggttaacac cacagttgcg attgctgctg aagtgttgaa aaaagccggt gtttatgaca
    39661 aaaacaaact gtttggcgtt accacgctgg atatcattcg ttccaacacc tttgttgcgg
    39721 aactgaaagg caaacagcca ggcgaagttg aagtgccggt tattggcggt cactctggtg
    39781 ttaccattct gccgctgctg tcacaggttc ctggcgttag ttttaccgag caggaagtgg
    39841 ctgatctgac caaacgtatc cagaacgcgg gtactgaggt ggttgaagcg aaagccggtg
    39901 gcgggtctgc aaccctgtct atgggccagg cagctgcacg ttttggtctg tctctggttc
    39961 gcgcactgca gggcgaacaa ggcgttgtcg aatgtgccta cgttgaaggc gacggtcagt
    40021 acgcccgttt cttctctcaa ccgctgctgc tgggtaaaaa cggcgtggaa gagcgtaaat
    40081 ctatcggtac cctgagcgca tttgaacaga acgcgctgga aggtatgctg gatacgctca
    40141 agaaagatat cgccctgggc gaagagttcg ttaataagta attgattagc gaataataaa
    40201 aaaccggagc acagactccg gttttttgtt ttgagcactc gacttaattg gttgccggat
    40261 attcctgaat ggtgacctgc agcgttaact gcttatcatc acgcatcact acaaccggga
    40321 tcaccgagcc agggcgaatt tctgccacct gatccatcgt ctccagagca gagatggccg
    40381 gtttgttatc caccgaaata atcagatcgt tgacctgaat acccgcattc gccgccgggc
    40441 cgtcaggtga cacttcatta accacgatcc cttgcagttg atctatacca ccgccctgcg
    40501 cgtgcagtgg tgcgatctcg cgtccgccga taccaatgta gccgcggatc acgcgaccat
    40561 cgcggatcag cttatccata attttggttg ctaactggaa aggaatcgca aagccgatac
    40621 cttccggcgt ttcgccatcg ttactcttat caaacgacag cgtgttaatg cccatcagtt
    40681 ctcccagcga gttcaccagc gcgccgccag agttaccgtg gttaatggaa gcatcggttt
    40741 gtaggaagtt ttgccgcccg gtcgggttca gaccgattcg acccgtggca ctaataatcc
    40801 cctgggtaat ggtctgcccg aggttgtacg ggttaccgat cgccagtacc acgtcgccaa
    40861 tgtgcggtac gcgacgtgca ttgatcggaa tggtaggtaa accgccagtg gcattaattt
    40921 taagtaccgc cagatcggtt agagagtcag atcccaccag caatgcttca aatacacgtc
    40981 catcctgtaa ggcgacgatg atctgatcgg cgtcgttgat gacgtgttta ttggtgatga
    41041 tataaccgcg ttgatccatg attacaccgg atcccagggt gcggatctca agctggttgt
    41101 gagagttggt gttcaaacca cggttgtaaa cgttaaccac cgctggcgcg gcgcggcgaa
    41161 ccgccagatt ataactggca ggcgtctcat cggtactgtc aaattgcgga gtggaaagcg
    41221 ggttaaggct gcgcagcgaa ggcatggcaa ccagcagaat agcgccgaca attaatccaa
    41281 tcgcaacgga acgtaagagc ttcacaaaca tgatggaggc gtcattaaaa aagggaacgg
    41341 cagcagcata ccacgagtta accggacatc acacgtcagc ctgatgcccg gtttacgaca
    41401 ttaacgcatc agcagataga tgctttcatt gccgcgtaca atttgcaggg cgatgatggc
    41461 cggttttgcc gccagcactt tacgcatttc agcaatcgag ttcacccgat cgcggttgac
    41521 gccaatgatc acatcgtctt tttgcaagcc agcctgagca gctgggcttc ctttgacaac
    41581 ttcatcgatt ttaatacctt tgccgccatc ttttagctga ccatcgctca acgttgcacc
    41641 ttccagcgct ggcgtgatca tttcagcgct ggccgacgaa gaggtgctgg tatcgagcgt
    41701 cacttctact tccagtggtt tgccgttacg cagcaggcca agcttcactt tcgtgcccgg
    41761 ctcggtggtc gcgatacgag agcgcaactc agcaaagcta ttcagcggtt tgccgttgag
    41821 gctggtaata atatcgcccg ctttgacgcc cgctttcgcc gagccagaac ctggcaacac
    41881 ttcgctgaca aacgcgccac gctgcacgtc aaggttgaag gctttggcga tatcggcact
    41941 catctcggtg cctttgatgc ctaacaaacc gcgtttgatt tcaccaaagt cgataagctg
    42001 ctgcgccagt gttcgcgcca tattactggg gatggcaaat ccaattccga cgctcccgcc
    42061 gccaggcgca aggattgcag tgttgatgcc aattaactca ccgttaaggt ttaacagtgc
    42121 accgccggag ttaccgcggt taatggaagc atctgtctgg ataaagtttt ccagaccttc
    42181 aagattcaac ccgctgcgtc ctaatgcgga aacaatgcca gaggtggcgg tttgcccaag
    42241 gccaaatggg ttaccgaccg ccacggcaaa atcaccgacg cgcaatttat cggagtcggc
    42301 aatagcgatt tgcgttaatt tgctcgggtt ttgaatttgc aacagggcga tatcgctctg
    42361 gtcatcgcta ccaatcagtt ttgcatcaaa ctcgcgccca tcattgagct gaatactgat
    42421 tttctgtgcc tgattaatca catggttgtt ggtcagcaca tagcctttac tggcgttgat
    42481 gatgacaccg gagcctaaac cttcgaaggg ttgtgcaggt tgatccggta aatcatcacc
    42541 aaaaaacttt ttgaattctt ccgggatttt ctgtccctga ctggccgttc cttccacccg
    42601 tacgctcacc actgccggaa gcactttttc cagcattgga gccagactgg ggaggggggc
    42661 ctgatcggca acctggcctg gaatcgacgc gacggcctga aatgacgccg agagagttaa
    42721 cccgacactt aacgctaatg cactcaacag ctgggtttgt tttttcatta ttcctgctct
    42781 cgtacctgaa tgataagaaa agagattcaa aacagtttga tgttattgaa ttttcagacg
    42841 gctaacaatg agacaccaga ttaaatgata gtcggggagg gagagaagag gagggcgcaa
    42901 tggctgcgcc cgaaaaataa attagtcgcg cttcgcgcca gtacgcagca ggccggatgc
    42961 gccttcagaa tagtcgcgag gcatctgcac cggtgcctga tcgttgctgg cttcagactc
    43021 tgccagacga ttacggaacg ggtttgcttc agcagacagt tccggcagca ggctgctgga
    43081 gcttttcgcc atgtgctgat acagctggcg atagtcgtgc gccatggtat ccagtaattc
    43141 cgcgctgcgg gcaaagtggc taaccagctc ttcgcgatac tcgtccagtt cagctttatt
    43201 cttttccagt tcgtactgca acgcctgttg ctggcgtagt ttacgattac caaaacgcat
    43261 ggccacagca ccaataatga tgccgacgac taacccaatt agcgcatatt cccaggtcat
    43321 gaacatctcc cgttgtcttt tgtttccgta gggtgttggc ttcaggctcc atgcctgcgg
    43381 ctgattatgc cactataacc gttaatgcca cagaagtgga atcccgactg catatcgcgt
    43441 agtgtagaac ggcctttttt tcgtcaaccg tgaacaacgg cgcaccgatt attcaaggaa
    43501 taacaataag atcatgcaaa gcgttacccc aacatcgcaa tacctgaagg cgctcaatga
    43561 aggcagccat caacccgacg acgttcaaaa agaggccgtc agccgcctgg aaattattta
    43621 tcaggaactc atcaatagca ggccaccagc ccccaggacg agtgggctaa tggcgcgggt
    43681 cggtaagctg tggggtaaac gcgaagacac aaagcatacg ccagtgcgtg gcttatatat
    43741 gtggggcggt gtaggacgcg ggaaaacctg gctgatggac cttttctatc aaagcctgcc
    43801 gggagagcgg aaacagcgcc tgcactttca ccgttttatg ctgcgggtgc acgaagagct
    43861 aactgcctta caggggcaga ccgatccgct ggaaattatt gccgatcgct ttaaagccga
    43921 aactgacgtg ctctgttttg acgaattttt tgtttctgat attaccgacg ccatgctact
    43981 tggcggtctg atgaaagccc tgttcgcccg cggtattacc ctggtagcga cgtcaaatat
    44041 tccgccggat gaactttatc gaaatggcct gcaacgtgcg cgttttctgc ctgcaatcga
    44101 tgccattaaa cagcattgtg atgtaatgaa cgtggacgct ggtgttgatt atcgactgcg
    44161 tacactcact caggcgcatc tgtggctttc gccactcaac gatgaaatcc gggcgcaaat
    44221 ggataaacta tggttggcgc tggcgggggc gaaacgagaa atttcaccaa cgttagaaat
    44281 caaccatcgg ccattagcga caatgggcgt cgagaaccag acgctggcgg tctcttttac
    44341 tacgctgtgc gtcgacgccc gcagtcagca tgactatatt gcgctctcac gtctctttca
    44401 tgcggtcatg ttgtttgatg taccagttat gacgcggttg atggagagcg aagcgcggcg
    44461 ctttattgcg ctggtggatg agttttacga gcgccatgtc aaattagtgg tgagtgcaga
    44521 agtgccgctg tatgaaattt atcagggtga gcggctgaag tttgagttcc agcgttgcct
    44581 gtcacgtctg caagagatgc aaagcgaaga gtatctgaag cgcgagcatt tggcgggtta
    44641 aaacctgcca caaatcacaa aaaggggtcg atctttgacc ccgacttctc tataatcctg
    44701 cgaccccacg ttacaagaaa gtttttttcc caaaactttt tgtgtgctgg cataggctat
    44761 tcgaaggggt aggtttgccg gactttgtcg tgtgaacctc aacaattgaa gacgtttggg
    44821 tgttcaccaa cgtgtaacta tttattgggt aagcttttaa tgaaaacttt tacagctaaa
    44881 ccagaaaccg taaaacgcga ctggtatgtt gttgacgcga ccggtaaaac tctgggccgt
    44941 ctggctactg aactggctcg tcgcctgcgc ggtaagcaca aagcggaata cactccgcac
    45001 gtagataccg gtgattacat catcgttctg aacgctgaca aagttgctgt aaccggcaac
    45061 aagcgtactg acaaagtgta ctatcaccac accggccaca tcggtggtat caaacaagcg
    45121 acctttgaag agatgattgc tcgccgtcct gagcgtgtga ttgaaatcgc ggttaaaggc
    45181 atgttgccaa aaggcccgct gggtcgtgct atgttccgta aactgaaagt ttacgcgggt
    45241 aacgagcaca accacgcggc acagcaaccg caagttcttg acatctaatc gggattatag
    45301 gcaatggctg aaaatcaata ctacggcact ggtcgccgca aaagttccgc agctcgcgtt
    45361 ttcatcaaac cgggcaacgg taaaatcgta atcaaccaac gttctctgga acagtacttc
    45421 ggtcgtgaaa ctgcccgcat ggtagttcgt cagccgctgg aactggtcga catggttgag
    45481 aaactggacc tgtacatcac cgttaaaggt ggtggtatct ctggtcaggc tggtgcgatc
    45541 cgtcacggta tcacccgcgc tctgatggaa tacgacgagt ccctgcgttc tgaactgcgt
    45601 aaagctggct tcgttactcg tgacgctcgt caggttgaac gtaagaaagt cggtctgcgt
    45661 aaagcacgtc gtcgtccgca gttctccaaa cgttaattgg cttctgctcc ggcagaaaac
    45721 aatttgcgaa aaaacccgct tcggcgggtt tttttatggc taaattttga attagcgtaa
    45781 aaaatggaaa gttgcttttt gctgccgtct gacagacagg caaaacaaaa accacatcgc
    45841 caataagggg ctaagtcaac tatttcagac taaagcgcat ctctttttcc ccatttccgg
    45901 catcgactca ccacaaaggt cgcaaaatct ggtaaactat catccaattt tctgcccaaa
    45961 tgtcgggtat tgctcatttt ttgtttgatt ttcgaacaaa gagagtcgtt ccttactggg
    46021 taacacaact tctgactggc cacctggtgg ctggtagcag taaaaattct gactatacct
    46081 ggaggttttc atggctgtcg ctgccaacaa acgttcggta atgacgctgt tttccggtcc
    46141 tactgacatc tatagccatc aggtccgcat tgtgctggct gagaaaggtg taagtttcga
    46201 gatcgaacac gtggaaaagg acaatccgcc tcaggatctg attgacctca acccgaatca
    46261 gagcgttccg accctggtgg atcgtgagct gaccctgtgg gaatctcgca tcattatgga
    46321 atatctggat gagcgtttcc cgcatccgcc actgatgcct gtttacccgg tagctcgcgg
    46381 tgaaagccgt ctgtacatgc atcgcatcga aaaagactgg tacacgctga tgaacaccat
    46441 catcaacggt tcagcttctg aagcagatgc cgcacgtaag caactgcgcg aagaactgct
    46501 ggcgattgcg ccggtcttcg gtcagaagcc gtacttcctg agtgatgagt tcagcctggt
    46561 cgattgctac cttgctccgc tgctgtggcg tctgccgcaa ctgggcatcg agttcagcgg
    46621 cccgggtgcg aaagagctga aaggctatat gacccgcgtc tttgagcgtg actctttcct
    46681 tgcttcttta actgaagcag aacgtgaaat gcgtctgggc cggagttaat ctgtatggat
    46741 ttgtcacagc taacaccacg tcgtccctat ctgctgcgtg cattctatga gtggttgctg
    46801 gataaccagc tcacgccgca cctggtggtg gatgtgacgc tccctggcgt gcaggttcct
    46861 atggaatatg cgcgtgacgg gcaaatcgta ctcaacattg cgccgcgtgc tgtcggcaat
    46921 ctggaactgg cgaatgatga ggtgcgcttt aacgcgcgct ttggtggcat tccgcgtcag
    46981 gtttctgtgc cgctggctgc cgtgctggct atctacgccc gtgaaaatgg cgctggcacg
    47041 atgtttgaac ctgaagctgc ctacgatgaa gatgccagca tcatgaatga tgaagaggca
    47101 tcggcagaca acgaaaccgt tatgtcggtt attgatggcg acaagccaga tcacgatgat
    47161 gacactcatc ctgacgatga acctccgcag ccaccacgcg gtggtcgacc ggcattacgc
    47221 gttgttaagt aatacaaaac aggccctggt ggcctgtttt gtcttttaat ggcttaataa
    47281 aatagcgtga ttgtggcaat catattcacc acgaaaccga ctgccatggg gatcgctgtc
    47341 cgcttcacca ccgcaaaagg cgaaacgcct gcaattcctg acgtaacgac taccaccgca
    47401 gtaatcggcg aaaccgcgcg cgctagcgtc gtggcaaaat gcatcggcat aatcattaca
    47461 accgctggta catgtagtcc ggctgcgata ttcggaataa gactggcaaa tgacataaac
    47521 ggcgcattgc cagagcccat cacaatggca caaatggcaa tgaccagcgc cataataatc
    47581 atcacgccaa taccgcccag accagaatgc tccgcacccc tgataaccgc atcgacagtg
    47641 ccaatcgtcg ttaagccttt cgcaaatatt tcacccgcga ccaccagcgt taccacgttg
    47701 gcaaactgcg tacccatgcc gtcaaaaaac gcctgcacat cgtccattgt ttcgcgtaat
    47761 ttatgcttgc ggaagaactc aacaaacatc gtcaccgtca aactcagtaa catcaccacc
    47821 accagatgca gttctgactg catcagcccg atgtgggcaa ggaatagcga gccgagcatc
    47881 aggattaacg gcatcacagg taaaatggcg taatagagcg gcgggacatt atcgagagtt
    47941 ttttgctctg cctgttcgtg attgatattt ttatcttttt tgtcaaaagc gcgttgcacg
    48001 aaaaagtggg agatcgccac cgagataatg acgcaagagg cgaccggaag ctggtagtgg
    48061 aagaagtaag tggcaatttt cattcccgcg acctgggctg caaaaatgga gttcgtttcc
    48121 agaatccccc actcaatgga catcgtggtt gcgataaccg ccaccgcaga gaggcgactt
    48181 actcccagac tcaccagcgt cggaaataag gtgaccatca gcaacatacc cagaccggag
    48241 gcgctggtaa taaactgcgc catgatttgg ccgatgacgt aagttgccga cagaataata
    48301 tacggcgagc gaatgagttt taacgggcgg cttaacaagc tcaccatcgc gcgactggcc
    48361 cctatgcgct ccatgtagcg ggcataaccg cccaccgcca taatcgacag ccccaatccg
    48421 gcaatgcggt tggacagcat gcgcaggatt tcattataaa tatcgacgat caagtagccg
    48481 ctgcttttgg tgggatcgag tacaccaccg aaccctaacc agacaccgca catcatcagg
    48541 aagatacctg cggcagccag caccacctga ggtttgtagt ttttcaggat caaatagccc
    48601 atcgtaatta atacgatgac agatataatt atgccgaaca ttgttgaact ccgtgtcaaa
    48661 agaaaacggt caatcccata aatggcagat tgaaaacaat gatgttatat tttttgcaag
    48721 tctatttatg gtgctgatgt cgtgttttta attgtaggtg aggtgatttt tcattaaaaa
    48781 atatgcgctt atgattattt tgtaagaaca cattcataat attcataatg ctcgtgaata
    48841 gtcttgtaaa taattcaatc gggatgtttt tatctgcgtt acattaattt ttcgcaatag
    48901 ttaattattc cgttaattat ggtaatgatg aggcacaaag agaaaaccct gccattttcc
    48961 cctactttca atcctgtgat aggatgtcac tgatgatgtt aatcacactg accttacaga
    49021 atgggcctta tgaacgcatt tgattcgcaa accgaagatt cttcacctgc aattggtcgc
    49081 aacttgcgta gccgcccgct ggcgcgtaaa aaactctccg aaatggtgga agaagagctg
    49141 gaacagatga tccgccgtcg tgaatttggc gaaggtgaac aattaccgtc tgaacgcgaa
    49201 ctgatggcgt tctttaacgt cgggcgtcct tcggtgcgtg aagcgctggc agcgttaaaa
    49261 cgcaaaggtc tggtgcaaat aaacaacggc gaacgcgctc gcgtctcgcg tccttctgcg
    49321 gacaccatca tcggtgagct ttccggcatg gcgaaagatt tcctttctca tcctggtggg
    49381 attgcccatt tcgaacaatt acgtctgttc tttgaatcca gcctggtgcg ctatgcggct
    49441 gaacacgcca ccgatgagca aatcgatttg ctggcaaaag cactggaaat caacagtcag
    49501 tcgctggata acaacgcggc attcattcgc tcagacgttg atttccaccg cgtgctggcg
    49561 gagatcccag gtaacccaat cttcatggcg atccacgttg ccctgctcga ctggctgatc
    49621 gccgcacgtc caaccgtggc tgatcaggct ctgcacgagc ataacaacgt tagctatcaa
    49681 cagcatattg cgatcgttga cgcgatccgg cgtcatgatc ctgacgaagc cgatcgtgcg
    49741 ttgcaatcgc atctcaacag cgtctctgct acctggcacg ccttcggtca gaccaccaac
    49801 aaaaagaaat aatgccactt tagtgaagca gatcgcatta tcagctttct gtatggggtg
    49861 ttgcttaatt gatctggtat aacaggtata aaggtatatc gtttatcaga caagcatcac
    49921 ttcagaggta tttatggcaa cgaatttacg tggcgtaatg gctgcactcc tgactccttt
    49981 tgatcaacaa caagcactgg ataaagcgag tctgcgccgc ctggttcagt tcaatattca
    50041 gcagggcatt gacggtttat acgtgggtgg ttcgaccggc gaagcctttg tacaaagcct
    50101 ttccgagcgt gaacaggtac tggaaatcgt cgccgaagag gcgaaaggta agattaaact
    50161 catcgcccac gtcggttgcg tcagcaccgc cgaaagccaa caacttgcgg catcggctaa
    50221 acgttatggc ttcgatgccg tctccgccgt cacgccgttc tactatcctt tcagctttga
    50281 agaacactgc gatcactatc gggcaattat tgattcagcg gatgggttgc cgatggtggt
    50341 gtacaacatt ccagccctga gtggggtaaa actgaccctg gatcagatca acacacttgt
    50401 tacgttgcct ggcgtcggtg cgctgaaaca gacttctggc gatctctatc agatggaaca
    50461 gatccgtcgt gaacatcctg atctggtgct ctataacggt tacgacgaaa tcttcgcttc
    50521 cggcttgctg gcaggcgctg atggtggtat cggcagtact tacaacatca tgggctggcg
    50581 ctatcagggg atcgtgaagg cgctgaaaga aggcgatatc cagaccgcgc agaaactgca
    50641 aactgagtgc aataaagtca ttgatttact gatcaaaacg ggcgtattcc gcggcctgaa
    50701 aactgttctg cattatatgg atgtcgtgtc tgtgccgctg tgccgcaaac cgtttggtcc
    50761 ggtagatgaa aaatatctgc cagaactgaa ggcgctggcc cagcagttga tgcaagagcg
    50821 cgggtgagtt gtttcccctc gctcgcccct accgggtgag gggaaataaa cgcatctgta
    50881 ccctacaatt ttcataccaa agcgtgtggg catcgcccac cgcgggagac tcacaatgag
    50941 tactacaacc cagaatatcc cgtggtatcg ccatctcaat cgtgcacaat ggcgggcatt
    51001 ttccgctgcc tggttgggat atctgcttga cggttttgat ttcgttttaa tcgccctggt
    51061 actcaccgaa gtacaaggtg aattcgggct gacgacggtg caggcggcaa gtctgatctc
    51121 cgcagccttt atctctcgct ggttcggcgg cctgatgctc ggcgctatgg gtgaccgcta
    51181 cgggcgtcgt ctggcaatgg tcaccagcat cgttctcttc tcggccggga cgctggcctg
    51241 cggctttgcg ccaggctaca tcaccatgtt tatcgctcgt ctggtcatcg gcatggggat
    51301 ggcgggtgaa tacggttcca gcgccaccta tgtcattgaa agctggccaa aacatctgcg
    51361 taacaaagcc agtggctttc tgatttcagg cttctctgtg ggggccgtcg ttgccgctca
    51421 ggtctacagc ctggtggttc cggtctgggg ctggcgtgcg ctgttcttta tcggcatttt
    51481 gccaattatc tttgctctct ggctgcgtaa aaacatcccg gaagcggaag actggaaaga
    51541 gaaacacgca ggtaaagcac cagtacgcac aatggtggat attctctacc gtggtgaaca
    51601 tcgcattgcc aatatcgtaa tgacactggc ggcggctact gcgctgtggt tctgcttcgc
    51661 cggtaacctg caaaatgccg cgatcgtcgc tgttcttggg ctgttatgcg ccgcaatctt
    51721 tatcagcttt atggtgcaga gtacgggcaa acgctggcca actggcgtaa tgctgatggt
    51781 ggtcgtgttg tttgctttcc tctactcatg gccgattcag gcgctgctgc caacgtatct
    51841 gaaaaccgat ctggcttata acccgcatac tgtcgccaat gtgctgttct ttagtggctt
    51901 tggcgcggcg gtggggtgct gcgtcggtgg cttcctcggt gactggctgg gaacccgcaa
    51961 agcgtacgtt tgtagtctgt tggcctcgca gctgctgatt attccggtat ttgcgattgg
    52021 cggtgcaaac gtctgggtgc tcggtctgtt actgttcttc cagcaaatgc ttggacaagg
    52081 gatcgccggg atcttaccga aactgattgg cggttatttc gataccgacc agcgtgcagc
    52141 gggcctgggc tttacctata acgttggcgc attgggtggc gcactggccc caatcatcgg
    52201 cgcgttgatc gctcaacgtc tggatctggg tactgcgctg gcatcgctct cgttcagtct
    52261 gacatttgtg gtgatcctgc tgattggtct ggatatgcct tcccgcgttc agcgttggct
    52321 gcgcccggaa gcattgcgta ctcatgacgc tatcgacggc aaaccattta gcggtgccgt
    52381 gccatttggc agcgccaaaa acgatttagt caaaaccaaa agttaatcct gttgcccggt
    52441 ctatgtaccg ggcctttcgc taagggaaga tgtatgtcgt tacttgcaca actggatcaa
    52501 aaaatcgctg ctaacggtgg cctgattgtc tcctgccagc cggttcctga cagcccgctc
    52561 gataaacccg aaatcgtcgc agccatggca ttagcggcag agcaggcggg cgcggttgcc
    52621 attcgcatcg aaggtgtggc aaatctgcaa gccacgcgtg cggtggtgag cgtgccgatt
    52681 attggaattg tgaaacgcga tctggaggat tctccggtac gcatcacggc ctatattgaa
    52741 gatgtcgatg cgctggcgca ggcgggcgcg gacattatcg ccattgacgg caccgaccgc
    52801 ccgcgtccgg tgcctgttga aatgctgctg gcacgtatcc accatcacgg tttactggcg
    52861 atgaccgact gctcaacgcc ggaagacggc ctggcatgcc aaaagctggg agccgaaatt
    52921 attggcacta cgctttctgg ctataccacg cctgaaacgc cagaagagcc ggatctgacg
    52981 ctggtgaaaa ggttgagcga cgccggatgt cgggtgattg ccgaagggcg ttacaacacg
    53041 cctgctcagg cggctgatgc gatgcgccac ggcgcgtggg cggtgacggt cggttctgcc
    53101 attacgcgtc ttgaacacat ttgtcagtgg tacaacacag cgatgaaaaa ggcggtgcta
    53161 tgaccacgct ggcgattgat atcggcggta ctaaacttgc cgccgcgctg attggcgctg
    53221 acgggcagat ccgcgatcgc cgagaacttc ctacgcctgc cagccagact ccgcaggcac
    53281 tgcgtgatgc cttatccgca ttagtctctc cgttgcaagc tcatgcgcag cgggttgcca
    53341 tcgcttcaac cgggattatt cgcgacggca gcttgctggc gctgaatccg cataatcttg
    53401 gtggattgtt acactttccg ttagtcaaaa cgctggaaca acttaccaat ttgccgacca
    53461 ttgccattaa cgacgcgcag gccgcagcat gggcagagta tcaggcgctg gaaggcgatg
    53521 taaccgagat ggtgtttatc accgtttcca ctggcgtcgg cggcggtgta gtgagcggcg
    53581 gcaaactgct taccggccct ggcggtctgg cggggcacat cgggcatacg cttgccgatc
    53641 cacacggccc agtctgcggc tgtggacgca caggttgcgt ggaagcgatt gcttctggtc
    53701 gcggtattgc agcggcagcg cagggggagt tggctggcgc ggatgcgaaa actattttca
    53761 tgcgcgccgg acagggcgac gagcaggcgc agcagctgat tcaccgctcc gcacatgtgc
    53821 tggcaaggct gatcgctgat attaaagcca caactgattg tcagtgcgtg gtggtcggtg
    53881 gcagcgttgg tctggcagaa gggtatctgg cgctggtgga aacgtatctg gcgcaggagc
    53941 cagcggcatt tcatgttgat ttactggcgg cgcactaccg ccatgatgca ggtttacttg
    54001 gggctgcgct gttggcccag ggagaaaaat tatgatgatg ggtgaagtac agtcattacc
    54061 gtctgctggg ttacatcctg cgttacagga cgcgttaacg ctggcattag ctgccagacc
    54121 gcaagaaaaa gcgccgggtc gttacgaatt acagggcgac aatattttta tgaatgtcat
    54181 gacgtttaac actcaatcgc ccgtcgagaa aaaagcggaa ttgcacgagc aatacattga
    54241 tatccagctg ttattaaaag gtgaggaacg gattctgttt ggcacggcag gcactgcgcg
    54301 ccagtgtgaa gagttccacc atgaagatga ttaccagctt tgcagcgcca ttgaaaacga
    54361 gcaaaccatc aacttaaaac cgggaatgtt cgccgtgttt atgccgggag aaccgcataa
    54421 accaggatgc gttgtcggcg aacctggtga gattaaaaag gttgtggtga aggttaaggc
    54481 tgatttaatg gcttaaaact tagttcgttt taaagattct gcagacagtg tctgcagaat
    54541 tcgaaaccgc tgaaattaac ggagtctata gccttcttct agcaccgcct gtgtccgaac
    54601 gagttcatcc gcgagtcgtt tttcatcagg tagttgcatc ttatattcac tcgccagaac
    54661 ggtgttaggc aaaccagcca gtgcataatg cgcttctccg actcctttct ctgcacagag
    54721 aaccagaccg atgggcggat tttcatccgg tagcgtccag tgctctttgg cgtagttgag
    54781 atacatattc atctgtccgg catcgctata gctgaatttg cccactttta gatcgacgat
    54841 tagcaggcag cgtaaacggc ggtggaaaaa cagcagatcg acacgaaacc agttgtcatc
    54901 aatgcgtaac cttcgctgcc gaccaacaaa ggcaaaatca tccccaagtt ccagcatgaa
    54961 atccatcagg tggttgatca gcgcctcctc aaaatcggat tctgagtatt catctttcaa
    55021 ttccagaaat tcgagcacaa agggatcgcg tatcgcctgt tgcggaagaa tatgcgtctc
    55081 ggcaggagcg tgttgttgca gcatggctga tttgtcatgt gacagcagtg ttcgctcata
    55141 aaattgggtc gcaatttgcc gttctagctg gcgaacagac cagccacagc ggagcgtctc
    55201 tttttcataa aggctgcggg cgtcagcgtt tttaacggaa agtaaacgga cgtaggtgga
    55261 ccagggcagg gggaaagatt tggcagacag cgtctgccaa atctttgccg atgggaattc
    55321 tgcagatggt gtctgcggaa ttcccaatgg tgttaattcg ccagacactg tctggcgaat
    55381 ttcaacatgt tgaaaaaaga ggtaaaaaag cctcatttgg cgtaagtttt ttgcagagaa
    55441 cccacgctta taccttagac ttaaatcctt tgatagtcgc ttgattagct gcgcaccata
    55501 cgcagccctg gcctcgccac cttgttcaaa ttcgacaatt cgtcggccaa tttcccagta
    55561 tgtcgcggtc atcagcgcgt taacgctacg taccgtttcc gtccgggcgc tatcgaccag
    55621 atgaataatg ccgtcgtgga tttgctggta gcctgttgag gttctctctg agagcgattc
    55681 cattatcttt ccctttatat ggcgcagtgt tgaataatgg aatcagctat acgccgggca
    55741 tctgccggta acaatccaga tgtgcgtatt acattggttt tcttcagatt atgcgaggcg
    55801 ctttccagaa cgaaaggaga atttcagcgc cgactctttt ctgaggaggc ggcgctggga
    55861 cagagcttaa acttccagcc agttcataat gccgtctgcc gccttacgac cttcggcaat
    55921 agcggtaacg accagatcgg aaccacggac gatatcgccg ccagcaaaga ttttcgggtt
    55981 gctggtctgg aaagcgttgt cgctgccttc cggggcgatg atgcggcctt gcgaatccag
    56041 ctcgacgctg tgttttgcca gccattccat gctgtgtgga cggaaaccaa acgccatgat
    56101 caccgcatct gccggaacga tatgttcgga accagccacg atctccgcgc ggcgacggcc
    56161 tttggcgtcc ggttcgccca tttcggtacg caccattttt acgccgctga ctttgccgtt
    56221 gccgttcact tcaataccca gcggctggac gttgaatttg aactcaacac cttcttcccg
    56281 cgcgtttttc acttcgcggc gggaacctgg catgttctct tcatcacgac gataggcaca
    56341 ggttacgtgc ttcgctccct ggcgtacaga cgtgcgcacg cagtccatcg cggtatcgcc
    56401 accgccaagt actaccacgc gtttaccttc catgctgacg aacggttcgt cgttggtttc
    56461 accaaagccc attaactgtt tggtgttggc gatgaggaac ggcagcgctg cgtacacgcc
    56521 atcagcgtct tcgttttcca gcccgccgcg cattgactga taagtcccga cgccaaggaa
    56581 cacggcatcg taatcactca gcagatcgtc cagctgtacg tcgcggccca cttcggtatt
    56641 gagtttgaat tcaataccca tgccggtgaa gatttcacgg cggcgcgtca ttacctcttt
    56701 ttccagtttg aaagccggaa taccgaaggt cagcaagccg ccaatttccg ggtgacggtc
    56761 aaagacgacc gcttgtacgc cgttgcgcgt caggacatct gcacacgcca gacctgccgg
    56821 acctgcgccg ataatcgcca cttttttacc ggtctgtttc acgccggaca tatccggacg
    56881 ccagcccatt tcgaacgctt tatcattgat ataacgctca atgttgccga tggtcaccgc
    56941 gccaaactca tcgttcaggg tgcaggaacc ttcgcacaga cggtcttgcg ggcagacacg
    57001 tccgcatact tccggcagag tgttggtctg atgcgacagt tctgccgctt caaaaatacg
    57061 cccctcgttg gcgagcttaa gccagttcgg gatgtagttg tgtaccgggc atttccactc
    57121 gcagtatggg ttgccgcacg acaggcagcg gtcagcctgc gctttggcct ggccttcgga
    57181 aaacggctcg taaatttcaa caaactcaat tttgcggatc ttcagcggtt tcttaggcgg
    57241 atcaacgcgc tgcaggtcga taaattgata aacattctga ctcattgtag ctacccctta
    57301 ctgcgcctgc acgcgcaact ctgctgcgct acgactacgg tgacccagca gtgctttgac
    57361 atcactggac ttcggtttaa ccagcgcaaa tttagtggcg aaggctgacc agttcgccag
    57421 aatctcttca ccacgctggg aaccggtatg ctgcacatgc tcggtgataa gaccacgcag
    57481 atgctcttca tggatcgcca aatcgtcaac gcttaatact tccaccagtt ccgggttaac
    57541 gcgtttgcgg aaatcgccgc tttcatcgag aacgtaagcg aagccgccgg tcatacccgc
    57601 accgaagtta acgccggttt tacccagaat acagacgata ccgcccgtca tatattcaca
    57661 gccgttgtcg ccaatgcctt ctaccacggt gattgcaccg gagttacgca cgccgaaacg
    57721 ttcacccgcg cggcctgcgg catacagacg accaccggtc gcgccataca ggcaggtgtt
    57781 gccgataata cttgcttcat ggctgcggaa ggcggaacca accggaggac gaatggcgat
    57841 taagccgccc gccatgcctt taccgacata gtcgttggca tccccggtca ggtacagttc
    57901 cacgccgccc gcgttccaca caccgaaact ctggcctgcg gtgccgttga agtacgcttt
    57961 gataggatcg gctgccagcc cctgatcgcc gtgcgtctgg gcgatatagc ctgaaagcga
    58021 cgcgccgaca gaacggtcgg tgttgcggat atcgaaccag aaagttttgc tctggcgttc
    58081 atcgacaaac ggtttcgcct gttgcagcaa ctgcgcgttc agcaggccgt tatcaaacgg
    58141 cgggttggtt tcggtgcagt agagtgcctt acctggatgc ggttcggcgg tctccagcag
    58201 cttcgacagc gccagtttct gctgtttggc ggtgaaaccg tccagctctt tcagcaggtc
    58261 ggtgcgacca atcagatcca ccagacgtgt tacgccaagc tgtgccatca gctcgcgggt
    58321 ttcacgggcg ataaactcaa agtaattcgt caccttgaac ggcaggccgt gatagtgatt
    58381 cttacgcagt ttgtcatcct gagttgctac acccgtggcg cagttgttca ggtggcaaat
    58441 acgcagatat ttacaaccga gcgccaccat tgggccagtg ccgaagccga agctttccgc
    58501 gccgagaatc gccgctttga tgatatcgac acccgttttc aggccgccat cgacctgcaa
    58561 acggatctta tgacgcagac cattggcaac cagcgcctgc tgggtttcaa caagccccag
    58621 ctcccacggg caacctgcgt atttcactga cgacagcggg cttgcgccgg tgccgccgtc
    58681 atagcctgcg atggtgatta aatcggcata agctttcgcc acaccggtgg cgatggtacc
    58741 aacgcccggt tcggaaacca gcttcacgga gatcatcgct ttcgggttaa cctgcttgag
    58801 gtcgaaaatg agctgcgcta agtcttcgat agagtagata tcgtggtgcg gcggcgggga
    58861 gatcagcgtc acgcccggca ccgaatagcg cagtttggca atgtaaggag tgactttgtc
    58921 gcccggcaac tgaccgcctt cgcccggctt cgcgccctgg gcgactttaa tctgaatgac
    58981 gtcggcattg accagatacg ccggagtaac cccaaagcga ccggaagcca cctgcttgat
    59041 gcgcgacact ttgttggtgc cgtagcgcgc cgggtcttca ccaccttcac cggagttaga
    59101 gttaccgccg atgctgttca tcgcttccgc cagtgcctcg tgggcttccg ggcttaacgc
    59161 gccgatagac atcgcagcgg tatcgaagcg tttaaacagt tcgcttgccg gttcaacatc
    59221 cgcaatgttg accgcgtttt cacccggcgt aattgccagc agatcgcgca gcgtggttgc
    59281 cggacgctca ttaaccagct tcgcgtattc ctgatagtcg ctatactcgc cgctctgtac
    59341 cgcctgttgc agcgtgcgca ccacgtccgg gttgtaggcg tggtattcgc cgccgtggac
    59401 gtatttcagc aggccgccct ggctgatggg cttgcgcgcc agccaggcac gtttcgacag
    59461 attcagcaga tcctgctgga agtcttcaaa gctcgccccc ccaatgcggc tgaccgcccc
    59521 ctggaagcac aggcccacta catcatcgtg cagaccgacc gcttcaaaca gtttcgagca
    59581 gcggtaagag gcgatggtgg agatgcccat tttggacatg attttgtaca agcctttgtt
    59641 gatgccgtta cggtagttga gcatcacggt acgataatct ttggcaatcg cgtgggtgtc
    59701 taccaggcgg cccagcgttt cataggcaag gtacggataa atagccgtcg caccgaagcc
    59761 cagcaacacg gcgaaatggt gtggatcgcg ggcgctggcg gtttcgatga tgatgttggc
    59821 atcgcagcgc aggctttgat cgaccagacg ggtctggatc gcgccaaccg ccatcggagc
    59881 cggaaccggc aggcgatctt tagcgatatt ccggtcggag agcaccagca acacggtgcc
    59941 gctacgtacc attttttcgg ctttgtcgca cagctctttg accgtcgctt cgagcgtggt
    60001 tttggtgacg tcaaaggtga tatccagcgt atctgcgcgg tagtgctcct ctttcatcgt
    60061 cgtgagctgt ttgaaatcgg agtagagcag aatcggcgat ttaaagctta aacggtgcgc
    60121 ctggccctct gcttcgcaaa agacgttcat ttcacgacca atgctggtgg cgagcgacat
    60181 aacatgcgct tcacgcagcg ggtcgattgg cgggttagtc acctgggcaa actgctggcg
    60241 gaagtagtcg taaataatgc gcggctgact ggagagcacg gcgaatgggg tatcatcgcc
    60301 catcgaaccg accgcttcct gaccgttttc gcccagtacg cgaattacgg agtccagctc
    60361 ttccgcgctg tagttaaact gtttctggta gctggcaagc gtgtcgtcgt ccagttcgcg
    60421 gctacccact tcttcatcgg gcagatcttc aaacggtacc agtcggcgga cgtttttctc
    60481 catccactct ttataagggt ggcggctttt cagatcgtca tcggtttctg ccgagtgcag
    60541 aatacgccca ctgcgagtgt cgatcaccat cagttcgcct ggcccgacgc ggcctttttc
    60601 gaccacttcg tcaggctggt aatcccagat accgacttca gaggcgcagg tgatgagctt
    60661 atctttggtg atgacgtagc gcgccggacg cagaccgtta cggtcgaggt tacaggcagc
    60721 aaaacgaccg tcagacatca cgatgcccgc cgggccatcc cacggctcca tatgcatgga
    60781 gttaaagtca aagaaggcac gcagttccgg gtccatatcc gggttgttct gccaggcggg
    60841 tggtactaat agacgcatgg cgcggatgat atccatcccg cctgccagca gcagttccag
    60901 catgttatcc atcgaactgg agtcagagcc ggtttcgttg acgaacggtg cggcgtcgtg
    60961 caggtcaggg ataagcggtg tctggaattt ataggtacgc gcacgcgccc attggcggtt
    61021 accggtgatg gtgttgattt caccgttatg cgccagatag cggaacggct gcgccagcgg
    61081 ccagcgcggt acggtgttag tggagaagcg ctggtggaac aggcagatgg ccgattccag
    61141 gcgcaggtcc gcaagatcca ggtaaaagcg cggcagatcc gccggcatac acagaccttt
    61201 ataaatattg accagattcg acaggctaca gacgtagaag tctttgtcgg cttcgagacg
    61261 cttttcgatg cggcggcggg cgataaacag acggcgttcc atatcacgcg gacgccagcc
    61321 tgccggggcg ttcacaaaaa tttgctcaat gcgtggcaga gaggagaggg cgatttcacc
    61381 cagcacgcct tcgttagtgg ggacatcacg ccagcccaca atcgacaacg tttcgcgttg
    61441 cagctcttct tcaacgatgc ggcgtgcggc agcagcgagt tcaggatctt tattcaggaa
    61501 gagcatcccg acagcgtagt tttttgctaa acgccagccg cgctcctgcg caacgatgcg
    61561 aaaaaagcga tccggttttt gtaacagcaa gccgcaaccg tcaccggttt taccatcggc
    61621 gagaatcgcg ccacggtgct gcatgcgggc cagtgcgtgt attgcagtac gcactacctt
    61681 gtggctaggt tcgccttcta tgtgggcgat caggccgaaa ccacagttat ccctctcaag
    61741 ggatttatcg tacaacatat cgtgaacctc ccccaggctc tgcgggaacc ccactaccga
    61801 accgctgcgc acgggcacag aaagagcgtg gcgacggggt ttgcgtgtca tacgcgcgcc
    61861 tcgcattcgc ccccttgttg tccttttcgc atcgattaat acggtgaaga ggggatctca
    61921 attactgcat aaatataatg agcaggctgc tcatccagaa agcttccagc ggatttccaa
    61981 cttatcggga aagctgatac aggtcaaatg gcaagcttat tggtacagaa atgtgcttta
    62041 gcgcaaaatt ctttattaat tgactgaaat taaaatagat tttctaatta aggataaaag
    62101 gagcgtcgtt agagaaacag ccaattcctg actgtcataa aggcgtgtta acccttatcc
    62161 tgccttgaac tgacgagaaa accagcattt tatactgcct taattggtaa tgacgtttca
    62221 tgctactgtt tttgcctaaa atccataagg agataaaaaa atctgacaca agataagcaa
    62281 aagtgattta tgtgattatg catgaaattt cggtaatttt gattgattat gcagttggta
    62341 aaggcaggcc gggacgattg atccaggtca tcgccgaaat gagctaaaaa tggcaggctt
    62401 tggcctcttt tcaaggcgcg gtctatcaga ttatgcagtt acagaaatta gtcaatatgt
    62461 ttggtggtga tctcacccgt cgttatgggc aaaaggtgca taagctgacg ctgcatggcg
    62521 gttttagctg ccctaaccgt gacggtacca tcgggcgtgg cggctgcaca ttctgtaatg
    62581 ttgcctcgtt tgctgatgaa gcgcagcaac atcgttccat tgccgagcaa ctggcgcacc
    62641 aggcgaattt agttaaccgc gctaaacgct atctggccta ctttcaggcg tataccagca
    62701 cctttgcgga agttcaggtg ctgcgttcta tgtatcagca ggcggtgagc caggccaata
    62761 ttgtcggttt gtgtgttggt acccgcccgg actgcgtgcc ggatgcggtg ctggatctgc
    62821 tttgcgaata taaggaccag ggttacgaag tgtggctgga gctggggcta caaaccgccc
    62881 acgacaaaac actgcatcgc atcaaccgcg gccatgattt tgcctgttat cagcgtacaa
    62941 cccagctggc acgtcagcgc gggctgaagg tgtgttccca cttaattgtc ggcctgcctg
    63001 gcgaagggca ggctgaatgt ttgcaaacgc tggagcgcgt ggttgaaacc ggcgtggacg
    63061 gcataaagct gcatccgctg catattgtga aaggcagcat tatggcgaaa gcctgggaag
    63121 cggggcgttt gaacggtatt gaactggagg attacacact cactgccgga gaaatgattc
    63181 gccatacgcc accggaagtg atctaccacc gcatttccgc cagcgcccgt cgcccgacgc
    63241 tgcttgctcc gctgtggtgc gaaaatcgat ggacgggaat ggtcgagctg gatcgctatc
    63301 tgaatgaaca tggcgtacag ggatcggcgc tgggacgtcc gtggctacct cctacggagt
    63361 aggtcattga ggggaattcc gcatttctca cacaatttat aacgtaactg tcagaattgg
    63421 gtattattgg ggcaggttgt cgtgaaggaa ttccctaatg aagcaaattc gtctgctggc
    63481 gcagtattat gttgacctga tgatgaagtt aggtctggtg cgcttctcaa tgttgctggc
    63541 gctggccctc gtcgttcttg ccattgtggt acaaatggcg gtaaccatgg tgctgcatgg
    63601 tcaggtcgaa agcattgatg ttattcgttc tatcttcttt ggtttgctga ttacgccgtg
    63661 ggcggtctac tttctatcgg tggtcgtcga gcaactggag gagtcacgac aacgtctgtc
    63721 acggctggtg caaaaactgg aggagatgcg cgagcgcgat ttgagcctca acgttcagtt
    63781 aaaagataat attgcccagc taaatcagga aattgccgtt cgtgaaaaag cggaagcaga
    63841 actgcaggaa accttcggcc aactgaaaat tgaaatcaaa gagcgcgaag agacacaaat
    63901 tcagctcgag cagcaatcct cattcttacg ttccttcctt gatgcttcac ccgacctggt
    63961 tttttatcgt aacgaagata aagagttttc cggctgtaac cgcgcgatgg agctgctgac
    64021 cggaaaaagc gaaaaacaac tggttcatct gaaacctgct gatgtttact caccggaagc
    64081 cgctgcgaaa gtcattgaaa ccgatgaaaa agtgttccgt cataatgtgt cactgaccta
    64141 tgagcagtgg ctggattatc ccgacggacg caaagcctgc tttgaaattc gtaaagtgcc
    64201 gtactacgac cgcgtgggta aacgtcacgg tttgatgggc tttggtcgcg acattaccga
    64261 gcgtaagcgg tatcaggatg cgcttgaacg cgccagccgc gacaaaacga cgtttatttc
    64321 caccatcagt cacgaattgc gtacaccgct gaacggtatc gtcggcctga gccgcatcct
    64381 actggatacc gaactcaccg ccgagcagga aaaatatctc aaaactatcc atgtttcggc
    64441 cgtcacgctg gggaatatct tcaacgatat tatcgacatg gataagatgg aacggcgcaa
    64501 ggtccagctt gataatcagc cggttgattt caccagcttc cttgccgatc tggaaaatct
    64561 ctccgccttg caggcgcaac aaaaaggatt gcgctttaac ctggagccta cgctgccatt
    64621 accgcatcag gtcattaccg acgggacgcg tttacggcag atcctgtgga acctcatcag
    64681 taacgccgtc aaattcaccc agcaaggcca ggttaccgtg cgcgtgcgct acgatgaagg
    64741 cgatatgctg cattttgaag tggaagattc tggtatcggc attccgcagg atgagctgga
    64801 taaaatcttc gccatgtatt accaggtgaa agacagtcat ggcggtaaac ctgccaccgg
    64861 caccggtatt ggtctggccg tttctcgtcg tctggcgaaa aatatgggcg gcgatattac
    64921 ggttaccagc gaacagggca aaggttcaac ctttacgttg acgatccacg caccgtcggt
    64981 ggcagaagag gtcgatgatg cgtttgatga agacgatatg cctttaccgg cgctgaatgt
    65041 gctactggtg gaagacattg aactgaacgt gattgtcgcg cgttctgtgc tggaaaaatt
    65101 aggtaacagc gttgatgtcg ccatgaccgg caaggcggcg ctggagatgt ttaaaccggg
    65161 cgaatacgac ctggtgttgc tggatattca gttgccagat atgaccgggc tggatatctc
    65221 tcgtgaactg acgaagcgtt atccgcgcga ggatttaccg ccgctggtgg ctttaaccgc
    65281 taacgtgctg aaagacaagc aagagtacct caatgctgga atggatgatg tgctgagtaa
    65341 gccgctttct gttccggcgc taaccgcgat gatcaagaaa ttctgggata cccaggatga
    65401 tgaggagagt acggtgacga cagaagagaa cagtaaatca gaagcattgc tcgatattcc
    65461 catgctggaa cagtatctcg aacttgtagg accgaagctg atcaccgacg ggttagcggt
    65521 gtttgagaag atgatgccgg gctatgtcag cgtgctggag tcgaatctga cggcgcagga
    65581 taaaaaaggc attgttgagg aaggacataa aattaaaggt gcggcggggt cagtggggtt
    65641 acgccatctg caacagctgg gtcagcaaat tcagtctcct gaccttcctg cctgggaaga
    65701 taacgttggt gaatggattg aagagatgaa agaagagtgg cgtcacgacg tagaagtgct
    65761 gaaagcgtgg gtggcaaaag ccactaaaaa atgaccccgg ctagaccggg gtgcgcgaat
    65821 actgcgccaa caccagggaa atcttggctg cgccgtaaat tattatgatg agttacaagg
    65881 gcacagcact gtttttcagg ccgcacgcag ttaagatagc aaaacttaaa tgatttgtta
    65941 catgaatcag ttaaatgtgt gatgcgtacc atttttatta aaaaaattaa tgtgtttcag
    66001 tcaattgcga aaggatgatc acaatgaaga aaattggcgt aattctgagc ggatgcggtg
    66061 tctatgacgg ttctgaaatt catgaagcgg tgttgacgtt gttagctatt tcacgcagcg
    66121 gtgcgcaggc ggtctgcttt gcaccggata agcagcaggt tgatgttatc aaccatttaa
    66181 ctggcgaagc gatgacggaa acgcgcaatg tgctgattga agcggcacga ataacgcgcg
    66241 gtgaaatccg tcctctggcc caggccgatg ccgctgaact ggatgcgttg attgtgccgg
    66301 gggggtttgg cgcggcaaag aatttaagca attttgccag tcttggtcgc gaatgcaccg
    66361 ttgaccgtga attaaaggcg ctggcacaag cgatgcatca ggccggaaaa ccgcttggtt
    66421 ttatgtgtat tgccccggcg atgctgccga aaattttcga tttcccgctg cgtttgacca
    66481 tcggtactga tatcgatacc gcagaagtgc tggaagagat gggcgcagag catgtgccgt
    66541 gtcctgtcga tgatatcgtg gttgatgaag acaataagat tgtcaccacc ccggcatata
    66601 tgctggcgca gaacattgca gaagcggcga gcggcattga taagctggtt tcccgcgtgc
    66661 tggttctggc tgaatgagta aaagccgctt aacggtgttt agtttcgttc gccgttttct
    66721 tctgcggtta atggttgtcc tcgccgtttt ctggggcggg ggcatcgcgt tgtttagcgt
    66781 tgcgcctgtt cctttctcag cggtaatggt cgagcgacag gtcagcgcct ggctgcatgg
    66841 caattttcgt tacgtggcac attctgactg ggtcagtatg gatcaaatct cgccgtggat
    66901 gggactggcg gtgattgccg cagaagatca gaaatttcct gagcgctggg gctttgatgt
    66961 cgcttccatt gagaaagccc tggcgcacaa cgagcgcaat gaaaaccgta ttcgcggtgc
    67021 ttcaacgatt tctcagcaga cggccaaaaa tctcttttta tgggatgggc gtagctgggt
    67081 tcgaaaaggg ctggaagccg gattaacgct ggggatagaa acagtctgga gcaaaaagcg
    67141 tatcctgacg gtttacctga atatcgccga atttggcgac ggtgtgtttg gcgtcgaagc
    67201 tgcggcacaa cgctatttcc acaaacccgc gagcaaactt acccgatcgg aagctgcctt
    67261 actggcagct gtattaccta atccacttcg tttcaaagtc tccgcaccat cgggctacgt
    67321 gcgcagccgt caggcgtgga ttttacggca gatgtatcag ttaggcggtg agccgtttat
    67381 gcaacaacac cagctggatt aagcttcgcg cgaagtgagg gccagcgttt tgctctcacg
    67441 aggttgcaga tctttctgca gggcaatagc cagcgccggt tgggcgataa atcgtttcca
    67501 caaccgctct tgcttacgca gacaacacca ctttgaccag gtcgccagtg gaatcaatgt
    67561 tctttcacca atgttgtcgc agacgacggg gataacctct gactcactaa tgcggtgaca
    67621 gaggatattc tgcggcttca gcgacatcgt cacgatacgg ttatcctgca aataacgctt
    67681 cagctgtttc agtaattggc gcagttgtgc aacgtcttct tcgtaacggc attgttcagc
    67741 aaattcggtc agcgtaatgg agggcttgcc gtcaaaatcg gcaatcacat cgtagacata
    67801 accggttccg caatccgtct caacggtacc gtgatagcgc ggtattccac tccagtcttt
    67861 taaccggcga ccaagatgcg cgtagtactt taactcacgg cggatctctt tatcgccacc
    67921 atcgccacga tggtagacaa tcttgataca gcgttgggca tcttccggat gcgcataaca
    67981 tttccgatgt cttccggtgc ccaggggact ttgttcagat aagcgaatca tgccatctcc
    68041 tggaaatatt ttctgacgat ctccagtatg gtcgattaac ttaaacgaaa cctaaacaat
    68101 gcttcttaag gttttcttaa ggttcttctg aaagtaaaaa gcgcacgaaa tgtacaattg
    68161 acgttgattg agaacgattt cagtacgtga ttttcttatt gtcgggggga gtttgaaggg
    68221 agttgtatgt caaagtgatg aagattaatc ttcatcaaaa ccagaattaa agagggcgat
    68281 aacggcggcc agtgcttcct cttcctgtgg accggtcgct tcaacttcaa tctgccgtcc
    68341 tttggcagaa tccaacatca gcagcgcaat aacgctgttg gcttcagcct cggtgccttc
    68401 gtcattacgt aacagcactt cagcgtcaaa accctgcatt aattcaaaca gcttcattgc
    68461 aggccgggca tgcatgccca gcttgtttgt gatttcaaca gtttgcttga cggtcatggt
    68521 ttacgttttt ccagcgtacg atggcgtgac tggacgtttt taccgcgcga gcggaagtag
    68581 tcagccagtt gctctgcaat atacaccgaa cggtgcttcc cgccggtaca accaatggcg
    68641 accgtcaggt agctacggtt gttggtttcc agcataggta accatagctc aagatagctt
    68701 cgcgtctggt agataaaatt gtgtacttct gtgtggcggt cgaggaacgc ggcgacaggc
    68761 ttatcaagac ctgtcattgg acgcagtttc ggatcccagt gcgggttcgg caagaagcgc
    68821 acgtcaaaga cgtaatctgc atcgataggg ataccgtgtt tgaagccgaa agactcaaag
    68881 accattgtca gttcgcgttc acgtttaccc agcagacggg tacgcagcat ttctgccagc
    68941 tcgtgaacgg acatttctga ggtatcgaca atcagatccg ctcgcgaacg cagaggctcc
    69001 agcagatcgc tttctttgtc gatagcactt tccagcgaca ggtttttgct ggaaagcgga
    69061 tgcagtcggc gcgtgtcact gtaacgacga attaaggtat tacggtcggc atccaggaac
    69121 aggagttgcg gtgagaaagc gtcaggcagg ttactcatcg cctgttcgaa tatttctggt
    69181 gactccggca tattacgaac atcaatgctg acggcggcag aaatctcgcg atcggccagg
    69241 gttcgagcca gatcgggtaa caacactacg ggaaggttat ccacgcagta aaaacccata
    69301 tcttccagcg cacgcagggc gacagattta cctgaacctg aacgtccgct gacgatcatc
    69361 agtaccatgt actgtttctc ctcacaacgt ctaaaagaga cattaccgaa taactacgct
    69421 tcatccggag taccttcggt atccgtaatg atttgataca gctcttcatc gctctgggct
    69481 gcgcgtaaac ggcggcagat ggttttgtcc gccagacgtt tcgccaccag cgacagagta
    69541 tgcaggtgcg ttttagtttg gtctgccggc accagcaggg caaaaagtaa gtccaccggt
    69601 tggttgtcga tggcatcgaa agctataggc gtttcgagct gaacgaaaac gccaacggca
    69661 cgcagagtat cttcttccag tttgccatgc ggaatggcaa taccattgcc gataccggta
    69721 ctgcccattt tttcccgcgt caggatagct tcaaaaacca cctgaggcgg caggctaagt
    69781 tgtttcgccg ccagctcgct gatgatttcc agggcgcgtt ttttgctctg acagtggacg
    69841 cggcttcgcg tacattccct gttaagaaca ctgctaagct gtagagttgt atcattattt
    69901 gtcataattt cacctaagaa cctgcccact caaacagtgg aacaggctcg gagcattaac
    69961 cgtacaaatg gcccgttgtg ccgcacaaca ggccgccatg cacatgctaa ttgcccggac
    70021 aattagtgtt gtttcagttt atctttatgt ttggtcaact ggcgtgccag cttatcaatt
    70081 aagccatcaa tggcagcgta catgtcctga ccttccgcgc tggcatgaat ttcaccaccg
    70141 tttacatgca gtgttgcatc tgaggtgtgg gtgactttct ccactttcag aacaacatag
    70201 acctggttga ttcggtcaaa atattgctca agtttggcaa atttggctgt aacaaattcg
    70261 cgcagtgcct cggtgatctc gacgttattt ccggtaatgt tgagctgcat agtgtcttcc
    70321 ttatcggttg ggtcaaacga gctgtttacg ctggtttgac ggcggaatgg ataaagactc
    70381 tcggtacttc gcaacagtgc ggcgtgccac catgatacct tgttccgaca gcaaagaggt
    70441 taacttgctg tcgctcaacg gtttcgctgg gttttccgcc gcgattaatt tcttcaccag
    70501 cgcacgaatc gccgtggagg aagcttcgcc gccgccctcg gtattgacgt gactggaaaa
    70561 gaaatacttc agttcaaaaa tgcctcgtgg actatgcagg tatttttgcg tggtcacgcg
    70621 agatatcgtc gattcatgca tttcgacagc ctgggcgata tcggccagta ccatcggttt
    70681 catatattct tcaccttgct caaagaaggc ttgctgctgt tcaacgatac agcgactcac
    70741 gcgcagtagc gtatcgttac ggctttccag actcttgatc aaccatttgg catcctgcag
    70801 attgctgcgg ataaactggc tatcaccatc gttgcgcgcg ttattgcaca tcgaggcgta
    70861 gtgctggttg atttgcagac gcggaatgct gtcactgttg agttctaccg tccagtgacc
    70921 gttatgctta cgcaccagca catctggaat gacatactca ggttcgccag tctggatcga
    70981 ttgcccgggg cgcggatcga gcgactggat cagattgacg gcttctttca gcacatcttc
    71041 tttcagacgc gtgacgcgca ttaaagtgcg gaagtcgtga ttggctaaca gatcgagatg
    71101 atcgctaatg atcagtctgg cctcttccag ccacggcgtg gttttatcga attgggagag
    71161 ttggatcagc aggcagtcac gcagatcttt tgccgccaca ccgaccggat caaaccgttg
    71221 gatccgctta aggacggctt caacttcgtc gatgtcgatc tcttcatcgc ccatactttc
    71281 gagaatatct tccagcggga cagtcagata accggtgtca tcaacggcat cgacgataga
    71341 ggtagcaata gcgcggtcag tgtcggaaaa cggtgtcagc tcgacctgcc acatcaggta
    71401 atcctgcaag gtctgcgtcg tttcgccctg atagaccggc agctcgtcgt caatgtagtc
    71461 accgctggtg ccggatggtg taccagcggt gtaaatggta tcccaactgg catcgagcgg
    71521 cagctcttcc ggcatctctt tttgttcgag cgcgtcggcg gtgtccagcg tttcactgtc
    71581 ttgcgtttcg cgggtgtcga tttcttcatg agtgtcgatt tgctcaagca gcggattact
    71641 ctccagcgcc tgctgtagct cctgctgaag ttccagcgtc gacaactgca acagacgaat
    71701 tgcctgttga agctgtggcg tcatcgccag ttgttggcta agcctgagtt gcaaaccttg
    71761 cttcatgttc agaatcgtac tctcctgcta aaacgtcgca aacttctacc ctatcagagt
    71821 ctgaagtctt ccccaaggta tacacgctta acgtgttcgt cttgtaagat ttctgtaggc
    71881 gtgccgtggg cgatcaaatg cccctgactg acgatataag cgcgttcaca aaccgccagt
    71941 gtttcacgca cgttgtggtc agtgatcagc acgcccaggc cgctgtcgcg caggtgctca
    72001 atgatgcgtt taatgtcgat aaccgagatc gggtcaaccc cggcaaacgg ttcgtcgagc
    72061 agaataaatt tcggattcgc agccagtgcg cgggcaattt ctacacggcg acgttcaccc
    72121 ccggagagtg actgccccat gctgtcacgc aggtgctcaa tgtgaaactc ttccatcagc
    72181 tcgttcgcgc ggtcttcacg ttgttcagca gacaagtcgt cacgaatttg cagtaccgcc
    72241 atcaggttat cgtaaacgct gaggcgacgg aaaatggagg cttcctgtgg cagatagccg
    72301 ataccgcggc gcgcgcgtgc atgcagaggc agcagactga tatcgtcatc atcaataatg
    72361 atgttgcccg catcgcgcgg cacaatgcct acaaccatgt agaaagtggt ggtcttaccg
    72421 gcaccgtttg gccccagcag accgacaatt tccccggagt tgacggtcag gctgacgtct
    72481 tctaccacgc gacggccttt ataggctttt gcaaggttct ttgcagttaa tgttgccata
    72541 acgaattaat tacccttctt ctgtgccggg gtctggcctt tgttgttttt gtcctgcagc
    72601 tgcgacggta ccagaacggt ggttacgcgc ttgcctttgt cgctgaaagc ctgcattttc
    72661 tgctctttca ccaggtaagt gatcttatcg cccttaatgt tgctatcgac ctgctgcaga
    72721 taagcattac ccgtcagaac gacaaaatct tttgccagtt cgtagtgcat ctgggaagcg
    72781 tgaccttcaa cgggtttacc gttgtcctgc atctggtaga acgttgccgg tttaccgtag
    72841 ccgtcaatca cttctttacc ttgttcgccg cccggacggg taacgaccac tttgtcggca
    72901 ttaattttga tggtgccctg ggtgacgatg acattaccgg taaaggtaac cacgttgcct
    72961 tgcatatcaa gagattgctg gtccgattca atgtggatcg gctgatcagt gtctccggtt
    73021 acggcaaatg ccggaatgct ggcggccaga agtgagctgg caagcacaag attaaggctg
    73081 agtttgtttg ttttgaattt cataggatgt tctaaccttt tcaatcagct cggcgttctt
    73141 gctgcgtaag ttgccgcgca ttttcagacc gctggagtta aatgttgttc cgtataacgt
    73201 gacgaggtct tcagaggtaa catcctgcgt caccagattg atctgcgcgt tatccgtcgt
    73261 gattctgcga agttgagagt ccggcacaag tgcgttgact tcaacgtgtc catataaata
    73321 gagcatccgg tcattggtca gcttggcttt atctgctttt acggaccatg tcgggatttt
    73381 atccttatca aacgtggtaa gtaccggctg cgtaaaccac gaaacggcct gatcggaata
    73441 atattcaacg tgttgagcaa tcaatcgata gcttagcgcc ccttctgggt tatagacgag
    73501 cgtgtccgta tgctcgcttt tataggtggg atcattgttg ttgacgacca cctgggcggt
    73561 atcgtctttt tcggccatat taataccgat catcaccaga accgccagtg atagcacaat
    73621 gataacccaa cgtctggctt tactcatatc gattgccctt tggcttcatc cagtttgccc
    73681 tgcgccagga gtaataagtc gcaaacttct cgcactgcgc cacgaccgcc agcaatgcgc
    73741 gtcacgtaat cggcgcgcgg gatcaacagt ggatgcgcat cggccacggc gacgcttaaa
    73801 cccacttttt ccattaccgg ccaatcgatg agatcatcgc cgacataagc cacattttcc
    73861 ggggcaatcg ccaatttttc cagcagatcg ctaaaggcga tcagtttgtt tgactgcccc
    73921 tgatacaagt gagtgatccc caatgtggca caacgatctt ctaccagttt agcctttcgc
    73981 ccggtaatga tagcgacttc aatatcagag gtgagcgcac aacgaatgcc ataaccgtca
    74041 cgaacattga acgctttcag ctcttcgccg ttattgccca tataaatcag gccatctgac
    74101 agtacgccat caacgtcgag gattagcaga cgaatgttct ctgcttttgc tataacgtcg
    74161 gcgctgacag ggccgtaaca ggtcgcaagc gacgcacctg ctttgctcat tgttgtttat
    74221 ccttgaatct ttacactacg cctgcacgca gtaaatcatg catatgtaac acaccgagta
    74281 aatggtcgcc atcggcaacc atcacggagg tgatatggcg ggactgcatt aagttcagtg
    74341 cctcaacggc cagaatgcca gggcgcacgc gtattccccc cggcgtcatc acatcggcaa
    74401 tacttaactg acgaacatcc acgcccatat cgaagacacg gcgtaaatca ccgtcggtaa
    74461 agatgccttc aatcatcata ttgtcatcgc aaatgacagt cataccaaga tttttgcggg
    74521 taacttccag caatgcgtca cgcagactgg ccgttttctt aacatgcggg atctcatcgc
    74581 ccgtatgcat aatatcgttt acgcgcagca gaagtttacg acccagtgcg ccgcctgggt
    74641 gtgagagcgc aaaatcttca gcagtaaagc cgcgtgcttt taacagcgcg acagcgaggg
    74701 catcgcccat aaccagcgtg gcggtggtgc tgctggtcgg tgccagccct aacggacagg
    74761 cttctttcgc tactttaaca cacagatgca catctgcggc gcgcgccatg ctgctctccg
    74821 ggcgaccggt gatgcagatt aacggtacgt gaagacgctt aagcactgga attaaggcag
    74881 tgatttcgct ggattcacct gagttagaga tagcaatcac cacatcctgt ggggtgacca
    74941 tgcctaaatc accatgcgcg gcttcaccag gatggacgaa aaatgaaggt gtaccggtgc
    75001 tggcaaacgt tgcggccatt ttgcgcccga tgtgccccga tttccccatc cccatgacga
    75061 caactttccc tttacaccag aacatctttt cacaggcaag cgtgaaattc tgattgatgt
    75121 attgatcaag ctccgccagg cattcacgtt caatcgccag gacttcttta cctgcttgct
    75181 gaaagtcaaa acccggttgt aactctacgt gcgacataat gcgtttccag ttattcaacg
    75241 agtattggcg ataaccagta caacatcgcc agccatacga taaatccgcc agttaataat
    75301 acccctacac cacggcccgg ttgcggggag cgccgccagc acagcaacgc aaaaataatg
    75361 ctcaccagca acatcacgct gtagtcacga ctgtacgcca gtggatcaat ctctcctggc
    75421 gttatcagcg cgggtaaacc caacacgatg acaatattaa aaatgtttgc gccaatgata
    75481 tttccgacag caatgtcgtt ttcacctttg cgaacccccg cgattgcggt tgccagttcc
    75541 ggcaggctgg ttccgatagc aattgccgtc agacccatcg tcaactcgct gatggcaaag
    75601 taattcgcca gcaccgtggc gttatcaacc accatccgcg tggccactgg catgatgata
    75661 agcgcaatgc cgagccataa aaacgcgacg ggcaatccgc cgtcacgcgg cagctctgca
    75721 agctgctctc tggtcaggct gtcagtcccc tgacgttcag cctgacgtgc aagtttaaca
    75781 atgaacagca gccatagcac agccagaaag aggagaaaga taccatcgct gcgactaagt
    75841 tgtccgtcat agagtacgga accggccacc acgctgacca acaacattaa gggtaattca
    75901 cggcgtagaa catcagaatg gacggtaaaa ggacgaacca gcgcagccag accgaggatc
    75961 agcaatatat tgataatgtt tgagccgagg gctgtaccaa cggctaaatc gcgttgttcg
    76021 tgcagagacg cggcaagcga gacgatgatt tctggtaacg atgtaccaat actgaccacc
    76081 gtcatgccga tgatcagcgg cgggatgcca aaggttcggc aaagaataga cgcggcaaaa
    76141 accaggcggt cggcactgta aacgaccaaa agtaaaccaa caattaacag tgccgtagct
    76201 aaaagcatct aacgtccttt cttcaggtat actcgccggt ccgctgaaga ttttcagaaa
    76261 gcc
//
LOCUS       DAZGCQ010000008        74907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000008 DAZGCQ010000000
VERSION     DAZGCQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.008"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..538
                     /gene="yfgH"
                     /locus_tag="ACHK07_000594"
     CDS             20..538
                     /gene="yfgH"
                     /locus_tag="ACHK07_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8100973.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            554..1093
                     /gene="yfgI"
                     /locus_tag="ACHK07_000595"
     CDS             554..1093
                     /gene="yfgI"
                     /locus_tag="ACHK07_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO8100974.1"
                     /translation="MKKVFLCAILVSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1186..2763)
                     /gene="guaA"
                     /locus_tag="ACHK07_000596"
     CDS             complement(1186..2763)
                     /gene="guaA"
                     /locus_tag="ACHK07_000596"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO8100975.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2832..4298)
                     /gene="guaB"
                     /locus_tag="ACHK07_000597"
     CDS             complement(2832..4298)
                     /gene="guaB"
                     /locus_tag="ACHK07_000597"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO8100976.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4460..5830
                     /gene="xseA"
                     /locus_tag="ACHK07_000598"
     CDS             4460..5830
                     /gene="xseA"
                     /locus_tag="ACHK07_000598"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO8100977.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5827..6042)
                     /gene="yfgJ"
                     /locus_tag="ACHK07_000599"
     CDS             complement(5827..6042)
                     /gene="yfgJ"
                     /locus_tag="ACHK07_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO8100978.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6111..7583)
                     /gene="der"
                     /locus_tag="ACHK07_000600"
     CDS             complement(6111..7583)
                     /gene="der"
                     /locus_tag="ACHK07_000600"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO8100979.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7701..8879)
                     /gene="bamB"
                     /locus_tag="ACHK07_000601"
     CDS             complement(7701..8879)
                     /gene="bamB"
                     /locus_tag="ACHK07_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO8100980.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8890..9510)
                     /gene="yfgM"
                     /locus_tag="ACHK07_000602"
     CDS             complement(8890..9510)
                     /gene="yfgM"
                     /locus_tag="ACHK07_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO8100981.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9528..10802)
                     /gene="hisS"
                     /locus_tag="ACHK07_000603"
     CDS             complement(9528..10802)
                     /gene="hisS"
                     /locus_tag="ACHK07_000603"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO8100982.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10913..12031)
                     /gene="ispG"
                     /locus_tag="ACHK07_000604"
                     /gene_synonym="gcpE"
     CDS             complement(10913..12031)
                     /gene="ispG"
                     /locus_tag="ACHK07_000604"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO8100983.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12058..13071)
                     /gene="rodZ"
                     /locus_tag="ACHK07_000605"
     CDS             complement(12058..13071)
                     /gene="rodZ"
                     /locus_tag="ACHK07_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO8100984.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13356..14510)
                     /gene="rlmN"
                     /locus_tag="ACHK07_000606"
     CDS             complement(13356..14510)
                     /gene="rlmN"
                     /locus_tag="ACHK07_000606"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO8100985.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14660..15091)
                     /gene="ndk"
                     /locus_tag="ACHK07_000607"
     CDS             complement(14660..15091)
                     /gene="ndk"
                     /locus_tag="ACHK07_000607"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO8100986.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15231..16085)
                     /locus_tag="ACHK07_000608"
     CDS             complement(15231..16085)
                     /locus_tag="ACHK07_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8100987.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRAP"
     gene            complement(16085..16906)
                     /locus_tag="ACHK07_000609"
     CDS             complement(16085..16906)
                     /locus_tag="ACHK07_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO8100988.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSAPPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16899..17528)
                     /locus_tag="ACHK07_000610"
     CDS             complement(16899..17528)
                     /locus_tag="ACHK07_000610"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO8100989.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17525..19906)
                     /locus_tag="ACHK07_000611"
     CDS             complement(17525..19906)
                     /locus_tag="ACHK07_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO8100990.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHTCGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGIVEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            complement(20070..22382)
                     /gene="pbpC"
                     /locus_tag="ACHK07_000612"
     CDS             complement(20070..22382)
                     /gene="pbpC"
                     /locus_tag="ACHK07_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO8100991.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARQGKAGKLRLQPDDPLLERPLMSSEAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQAQQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(22383..27344)
                     /gene="yfhM"
                     /locus_tag="ACHK07_000613"
     CDS             complement(22383..27344)
                     /gene="yfhM"
                     /locus_tag="ACHK07_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO8100992.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLEVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRCEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            27551..28396
                     /gene="sseA"
                     /locus_tag="ACHK07_000614"
     CDS             27551..28396
                     /gene="sseA"
                     /locus_tag="ACHK07_000614"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO8100993.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            28756..28881
                     /gene="timP"
                     /locus_tag="ACHK07_000615"
     CDS             28756..28881
                     /gene="timP"
                     /locus_tag="ACHK07_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO8100994.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(28913..28999)
                     /locus_tag="ACHK07_000616"
                     /pseudo
     CDS             complement(28913..28999)
                     /locus_tag="ACHK07_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="enhances serine sensitivity caused by inhibition of
                     homoserine dehydrogenase I; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
     gene            29438..29563
                     /gene="timP"
                     /locus_tag="ACHK07_000617"
     CDS             29438..29563
                     /gene="timP"
                     /locus_tag="ACHK07_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO8100995.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(29595..30371)
                     /gene="sseB"
                     /locus_tag="ACHK07_000618"
     CDS             complement(29595..30371)
                     /gene="sseB"
                     /locus_tag="ACHK07_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO8100996.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(30514..31797)
                     /gene="pepB"
                     /locus_tag="ACHK07_000619"
     CDS             complement(30514..31797)
                     /gene="pepB"
                     /locus_tag="ACHK07_000619"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO8100997.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVFACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(31856..32056)
                     /gene="iscX"
                     /locus_tag="ACHK07_000620"
     CDS             complement(31856..32056)
                     /gene="iscX"
                     /locus_tag="ACHK07_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO8100998.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(32068..32403)
                     /gene="fdx"
                     /locus_tag="ACHK07_000621"
     CDS             complement(32068..32403)
                     /gene="fdx"
                     /locus_tag="ACHK07_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO8100999.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(32405..34255)
                     /gene="hscA"
                     /locus_tag="ACHK07_000622"
     CDS             complement(32405..34255)
                     /gene="hscA"
                     /locus_tag="ACHK07_000622"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO8101000.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(34272..34787)
                     /gene="hscB"
                     /locus_tag="ACHK07_000623"
     CDS             complement(34272..34787)
                     /gene="hscB"
                     /locus_tag="ACHK07_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO8101001.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(34883..35206)
                     /gene="iscA"
                     /locus_tag="ACHK07_000624"
     CDS             complement(34883..35206)
                     /gene="iscA"
                     /locus_tag="ACHK07_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO8101002.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(35223..35609)
                     /gene="iscU"
                     /locus_tag="ACHK07_000625"
     CDS             complement(35223..35609)
                     /gene="iscU"
                     /locus_tag="ACHK07_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO8101003.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(35637..36851)
                     /gene="iscS"
                     /locus_tag="ACHK07_000626"
     CDS             complement(35637..36851)
                     /gene="iscS"
                     /locus_tag="ACHK07_000626"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO8101004.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(36963..37451)
                     /gene="iscR"
                     /locus_tag="ACHK07_000627"
     CDS             complement(36963..37451)
                     /gene="iscR"
                     /locus_tag="ACHK07_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO8101005.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(37722..38462)
                     /gene="trmJ"
                     /locus_tag="ACHK07_000628"
     CDS             complement(37722..38462)
                     /gene="trmJ"
                     /locus_tag="ACHK07_000628"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO8101006.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            38581..39384
                     /gene="suhB"
                     /locus_tag="ACHK07_000629"
     CDS             38581..39384
                     /gene="suhB"
                     /locus_tag="ACHK07_000629"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO8101007.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            39529..40383
                     /gene="yfhR"
                     /locus_tag="ACHK07_000630"
     CDS             39529..40383
                     /gene="yfhR"
                     /locus_tag="ACHK07_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8101008.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKNGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            40574..41854
                     /gene="csiE"
                     /locus_tag="ACHK07_000631"
     CDS             40574..41854
                     /gene="csiE"
                     /locus_tag="ACHK07_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO8101009.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVCFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(41846..42985)
                     /gene="hcaT"
                     /locus_tag="ACHK07_000632"
     CDS             complement(41846..42985)
                     /gene="hcaT"
                     /locus_tag="ACHK07_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO8101010.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(43145..44035)
                     /gene="hcaR"
                     /locus_tag="ACHK07_000633"
     CDS             complement(43145..44035)
                     /gene="hcaR"
                     /locus_tag="ACHK07_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO8101011.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            44171..45532
                     /gene="hcaE"
                     /locus_tag="ACHK07_000634"
     CDS             44171..45532
                     /gene="hcaE"
                     /locus_tag="ACHK07_000634"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO8101012.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            45529..46047
                     /gene="hcaF"
                     /locus_tag="ACHK07_000635"
     CDS             45529..46047
                     /gene="hcaF"
                     /locus_tag="ACHK07_000635"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO8101013.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            46047..46367
                     /gene="hcaC"
                     /locus_tag="ACHK07_000636"
     CDS             46047..46367
                     /gene="hcaC"
                     /locus_tag="ACHK07_000636"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO8101014.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            46364..47176
                     /gene="hcaB"
                     /locus_tag="ACHK07_000637"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             46364..47176
                     /gene="hcaB"
                     /locus_tag="ACHK07_000637"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO8101015.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAAIGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            47186..48388
                     /gene="hcaD"
                     /locus_tag="ACHK07_000638"
     CDS             47186..48388
                     /gene="hcaD"
                     /locus_tag="ACHK07_000638"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO8101016.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            48485..48907
                     /gene="yphA"
                     /locus_tag="ACHK07_000639"
     CDS             48485..48907
                     /gene="yphA"
                     /locus_tag="ACHK07_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8101017.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(48955..49827)
                     /gene="yphB"
                     /locus_tag="ACHK07_000640"
     CDS             complement(48955..49827)
                     /gene="yphB"
                     /locus_tag="ACHK07_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8101018.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(49839..50933)
                     /gene="yphC"
                     /locus_tag="ACHK07_000641"
     CDS             complement(49839..50933)
                     /gene="yphC"
                     /locus_tag="ACHK07_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8101019.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMEHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(50966..51964)
                     /gene="yphD"
                     /locus_tag="ACHK07_000642"
     CDS             complement(50966..51964)
                     /gene="yphD"
                     /locus_tag="ACHK07_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8101020.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(51988..53499)
                     /gene="yphE"
                     /locus_tag="ACHK07_000643"
     CDS             complement(51988..53499)
                     /gene="yphE"
                     /locus_tag="ACHK07_000643"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8101021.1"
                     /translation="MFTATEAVPVAKMVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSGLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEIRALRHKP
                     KLDDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITHPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASRILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(53522..54505)
                     /gene="yphF"
                     /locus_tag="ACHK07_000644"
     CDS             complement(53522..54505)
                     /gene="yphF"
                     /locus_tag="ACHK07_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8101022.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQFVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEITQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(54602..57883)
                     /gene="yphG"
                     /locus_tag="ACHK07_000645"
     CDS             complement(54602..57883)
                     /gene="yphG"
                     /locus_tag="ACHK07_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO8101023.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDSLDYRCNLALAMLEYNR
                     ADFPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASKLL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKALGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            58001..59194
                     /gene="yphH"
                     /locus_tag="ACHK07_000646"
     CDS             58001..59194
                     /gene="yphH"
                     /locus_tag="ACHK07_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO8101024.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(59257..60510)
                     /gene="glyA"
                     /locus_tag="ACHK07_000647"
     CDS             complement(59257..60510)
                     /gene="glyA"
                     /locus_tag="ACHK07_000647"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO8101025.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            60838..62028
                     /gene="hmpA"
                     /locus_tag="ACHK07_000648"
     CDS             60838..62028
                     /gene="hmpA"
                     /locus_tag="ACHK07_000648"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO8101026.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(62073..62411)
                     /gene="glnB"
                     /locus_tag="ACHK07_000649"
     CDS             complement(62073..62411)
                     /gene="glnB"
                     /locus_tag="ACHK07_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO8101027.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(62472..63806)
                     /gene="glrR"
                     /locus_tag="ACHK07_000650"
     CDS             complement(62472..63806)
                     /gene="glrR"
                     /locus_tag="ACHK07_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO8101028.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEMFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMVRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(63796..64509)
                     /gene="qseG"
                     /locus_tag="ACHK07_000651"
     CDS             complement(63796..64509)
                     /gene="qseG"
                     /locus_tag="ACHK07_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO8101029.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDALRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(64674..66101)
                     /gene="qseE"
                     /locus_tag="ACHK07_000652"
     CDS             complement(64674..66101)
                     /gene="qseE"
                     /locus_tag="ACHK07_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO8101030.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(66677..70564)
                     /gene="purL"
                     /locus_tag="ACHK07_000653"
                     /gene_synonym="purI"
     CDS             complement(66677..70564)
                     /gene="purL"
                     /locus_tag="ACHK07_000653"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO8101031.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPVHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIYADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            70822..72378
                     /gene="mltF"
                     /locus_tag="ACHK07_000654"
     CDS             70822..72378
                     /gene="mltF"
                     /locus_tag="ACHK07_000654"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO8101032.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQHMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(72375..72878)
                     /gene="tadA"
                     /locus_tag="ACHK07_000655"
     CDS             complement(72375..72878)
                     /gene="tadA"
                     /locus_tag="ACHK07_000655"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO8101033.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(72936..73571)
                     /gene="yfhb"
                     /locus_tag="ACHK07_000656"
     CDS             complement(72936..73571)
                     /gene="yfhb"
                     /locus_tag="ACHK07_000656"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO8101034.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            73780..74628
                     /gene="yfhH"
                     /locus_tag="ACHK07_000657"
     CDS             73780..74628
                     /gene="yfhH"
                     /locus_tag="ACHK07_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8101035.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT        17936 a        20584 c        18707 g        17680 t
ORIGIN      
        1 atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg tggcaatgtt
       61 agcgtcattc actctggcag gatgccagtc aaatgctgac gatcatgccg ccgatgttta
      121 tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta tttccattct
      181 tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag ccttcggcgc
      241 gcttattggc gcagtcgctg gcggtgttat cggccacaac gtcgggtctg gcagcaattc
      301 cggaacgacg gcaggtgcag ttggcggcgg agctgtaggc gcggcagcgg gttctatggt
      361 gaatgataaa accttagtgg aaggtgtttc tttaacgtat aaggaaggca ccaaagtgta
      421 tacctctact caggtgggta aagagtgcca gtttacgaca ggtttagccg ttgttattac
      481 cacgacgtat aacgaaacgc gtattcagcc aaataccaaa tgtcctgaaa agagctaata
      541 atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttggtctcct taagctatcc
      601 ggctatcgcc tcatcattgc aggatcaact ctctgctgtc gcagaagcgg aacagcaagg
      661 taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca gggaaatcca
      721 gcaagagaag caacgtcgcg caaatgccca ggccgccgct aacaaaagag cggcaacggc
      781 agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagccactg cggacaaaaa
      841 acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac aaaaactggc
      901 gctggcgaaa gaagaagccc gcgttaagcg cgaaaacgaa tttatcgatc aggaactgaa
      961 gcacaaagct gcgcaaaccg atgtggtgca atctgaagct gacgccaaca gaaatatgac
     1021 tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaaca aatcggacag
     1081 ctggtttaat taatcgatgt tactaacttc aagcctatga ttcttgaaga taaaaaaccc
     1141 tctgtagtaa cagagggttt tgttcattca tagtgcaggg tcaaatcatt cccactcaat
     1201 ggtagctggc ggcttgccgc tgatgtcata caccacgcgg gaaataccgt tcacttcatt
     1261 gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc agtgagcggt
     1321 cataaagtcg atggtttcga ccgcacgcag agaaacaacc cagtcatact tacgaccatc
     1381 gcccattacg ccaacggaac gtactggcag gaagacggtg aacgcctggc tgactttgtc
     1441 gtataggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc gcagcaggtc
     1501 acagtactct ttcttcactt cacccagaac acgaacgcca aggcctggtc ccgggaacgg
     1561 gtgacggtac agcatgtcgt acggtagacc cagctccaga ccaatcttac gcacttcgtc
     1621 tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg gcaggccgcc
     1681 cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagacgccg cagattcgat
     1741 aacgtcaggg tagatggtgc cctgcgccag ccacttcacg tcctccagtt tcagcgcttc
     1801 ttcatcgaat acttcaacga aaacgcgacc gatgatttta cgttttgctt ccggatcgtt
     1861 ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa tgttcagacc
     1921 aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt
     1981 gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg tgcagcagca ttgcggttac
     2041 ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta cctgctcgcg
     2101 gatgcgagct acagcatcgt cgataatttt cgctggcgtc cacagggctt cacactggca
     2161 gatatcacgc acaaaacgct ccagcatgcg cataccctgg cgggtgtgag tcacttccgg
     2221 gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa acgggcagct
     2281 ttcggtgctg gctacggtga tgaagtcgga cgggatagcg gtaactttat cgccgtggct
     2341 catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac
     2401 cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt cgttagaggc
     2461 ttcaacgtga ccgcccaact gcattgccat ggtctgcatg ccatagcaaa cgccgaatac
     2521 cggtacgcct gcttcaaaga catactgcgg cgcacgcgga ctgttttctt cagtagtact
     2581 ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt gtgcttctgt
     2641 cacatcccac gcccacagtt cgcagtaaac acccagctca cgcacgcggc gcgcaaccag
     2701 ttgagtgtac tgagaaccga agtccagaat gaggatgcga tgcttatgaa tgttttccgt
     2761 cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga catgaagtcg
     2821 ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat ggtcacgtcg
     2881 tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc tttagtacgc
     2941 agttcgtcga tagtaccaca gccggtcaga cccatacagg agcgcaggcc gcccatctgc
     3001 tggtgaatga tctctttcag gcgaccttta taagctacgc gaccttcgat accttccggc
     3061 accagtttgt cggcagcgtt atcgctctgg aaataacggt cagaggaacc tttggacatc
     3121 gcgcccaggg aacccatacc acggtaagat ttgtaagaac ggccctggta gagttcgatt
     3181 tcacccggag attcttcagt acccgccagc atggaaccta ccatcaccgc gcttgcgcca
     3241 gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc gataaccgga
     3301 ataccggtgc cttccagggc ttcaactgcg tcagcaacgg cagtgatctg cggaacacct
     3361 acgccagtaa cgatacgagt cgtacagata gaacctgggc cgataccgac tttaaccgcg
     3421 ctgcaaccag cttctgccaa agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg
     3481 atttgcagat ccggatattt agcacgggtt tcacggatac gttgcagtac accttctgag
     3541 tgaccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca
     3601 cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg gccttgctcg
     3661 tctttacagg cgttcggttt acgttccgct ttctggaaat ctttcacggt gatcatgccg
     3721 atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc gtgcattttt
     3781 gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc tttcggcgtc
     3841 atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg accggtgata
     3901 atacccacca gttcgttttc ttcggtaacg accggatagc ccgcaaaacc gttacgctcg
     3961 gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc
     4021 acgccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc aatggacatg
     4081 tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag gcgcgcttcc
     4141 gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt tttcgtcagc
     4201 tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac gaggagaacg
     4261 tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc gaccagagtg
     4321 gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta cccctttttg
     4381 caaaaaatgc ttgctatccc cgaagggcgg gttactatcg actgaataac ctgctgattt
     4441 agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta ccgttagtcg
     4501 cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt ggatcagcgg
     4561 cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac tcaaagacga
     4621 caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga ccttccgccc
     4681 acagcatggg caacaagttt tagttcgcgc caatattaca ctctacgagc cgcgcggcga
     4741 ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc aacagaagta
     4801 cgaacagctc aaagcgaagt tgcaggctga aggattgttc gatctgcaat acaaaaaacc
     4861 acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca
     4921 tgatattttg catgtgttaa aacgtcgcga tccttctctg ccggtgatca tctaccccac
     4981 cgccgttcag ggcgatgacg cgccggggca aattgttcgc gccattgaac tggcgaatca
     5041 gcgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg
     5101 gagttttaac gacgaacgcg tagcgcgggc gatttttgcc agccgcattc cggtcgtcag
     5161 cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc tgcgtgcacc
     5221 aacaccgtcg gctgccgccg aagtggtgag ccgtaatcag caagagttac tgcgccaggt
     5281 gcaatcgacc cgtcaacggc tggagatggc gatggattat tatctcgcca accgcacacg
     5341 tcgctttacg caaattcatc accgattaca gcaacagcat ccgcagctcc ggctggcacg
     5401 ccagcaaacc atgcttgaac gcctgcaaaa acggatgagc tttgcgctgg aaaatcaact
     5461 taagcgtgcc gggcaacagc agcagcgatt aacacagcgg ctgaatcagc aaaatccaca
     5521 gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac
     5581 cctgcgcgca cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga
     5641 agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg ctactgacgg
     5701 caatgtactg aaaaaagtga agcaagttaa agcgggtgaa atgctaacca cacgtctgga
     5761 agacggctgg atagaaagtg aagtaaaaaa catccagccg gtaaaaaaat cgcgtaaaaa
     5821 ggttcattaa gccagcacga attcaacgcg ttttttcgaa atcaagccat gcccgtgctg
     5881 acagaagtaa tctaccgcac cgcaggcttt taacacctgc aacggttgat ggcagtccgg
     5941 gcaaagagct ttcatttcaa taacttcccc gcaggatggg caatgcgcat ggccgttatc
     6001 ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc ctccggtagc
     6061 cacccctcta cattcataga gggaatggca gataaaatac ttacggataa ttatttattt
     6121 ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta
     6181 cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg cgatcccatt
     6241 acgtccagcg atttgcggaa gtagttcatc aagtaacgct tgtaggaatc aggcaggtct
     6301 ttcacctgat taccgtgaat caccacaatc ggcggattat aaccaccggc gtgtgcatat
     6361 ttcagcttca cacgacgacc gcgtaccagc ggcggttggt gatcttcaac agccatcgtc
     6421 atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata cgcttcacgt
     6481 actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa gtgcacacga
     6541 gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc tttcacttcc
     6601 tggctcaggc catcccactt attcaccaca atgacaagtg agcgcccact attgagaata
     6661 aagcccagca gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat cactaacatc
     6721 accacgttgg cgtcttcaat ggcctgcaac gttttgatta cggagaattt ctctacagca
     6781 tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca
     6841 tcgcgttcca ttggaatgta gatactgtca cgcgtcgtac caggcatgtc ataaacaaca
     6901 acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt cggacgaccc
     6961 acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg
     7021 ccgttctctt ccgcttcaaa ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc
     7081 ggtgcgagat cttccatcca cggcagtagc acatgctcca gcagacttaa tacgccacga
     7141 ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa gcgagtagaa atcaaccact
     7201 gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg tttttcacgg
     7261 gagcgcagat gtttggcaat cgcttcatct gccggcatca ggcccgcgcg cgcatccacc
     7321 ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc catgcgggtt
     7381 tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tacagataaa ctcacggcct
     7441 tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc cgcaaccagc
     7501 gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt agggcgcccg
     7561 acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat cagacaacgc
     7621 acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg agccgttttc
     7681 aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc ttttgcctgg
     7741 atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact atcaactttt
     7801 tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcaaataacc ttcactgtca
     7861 ccgaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg cagcagatcg
     7921 ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg gtcattttga
     7981 tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt
     8041 ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt ataggccagc
     8101 gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag acggtcaatt
     8161 tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc
     8221 agcactgcgc tgacgcgacc attatcgccc cccacgacgg ccgcaccaaa agccgttgcc
     8281 ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt ccatttgaca
     8341 gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat taacaccaga
     8401 ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg ccatgccaca
     8461 gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc
     8521 ccaccagaca cggtcacacc gccagaaagt aatgcaggct ctttagagaa ccagccatct
     8581 ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag cgcttttact
     8641 aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg aagattggaa
     8701 tagaagttgc caatgccgct accaacggaa gtgctccacg ccgtggtcgg cgtaaactgg
     8761 ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt aaacagcgaa
     8821 cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg caattgcatc
     8881 gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc tcagtgccgg
     8941 agtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct tatcaccttt
     9001 gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt cacctttgat
     9061 ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag
     9121 acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc cctgttgtaa
     9181 ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga
     9241 agccagcgca ccataagtat ttttattttc agcagcaaat ttttctgccg ccgggatgct
     9301 atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag aagcggagcg
     9361 tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc caacgcccaa
     9421 aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac
     9481 ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta acccagtaac
     9541 gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc agagcgcaaa
     9601 tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac agcaacgcgg
     9661 gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc
     9721 atcaatttca cgcccggtaa ttcatcacgc agacgctcag ctaatgccat agccgcagat
     9781 tgtgtatcag cacctgaagc caccaggtat atatcgacaa caggatcggc tttaaattcc
     9841 ggattaacgg cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa accgacagcc
     9901 ggtgttgcac ggccgcccag ttgttccaca agaccgtcat aacgaccgcc tgcacacacg
     9961 gtgccctggg agccgagact gttagtcacc cactcgaaaa cggtacggtt gtagtaatcc
    10021 agaccacgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc aagcagtttg
    10081 cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcacctaa tgccggagcg
    10141 tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag cgggttagtg
    10201 tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag gaatgccacc
    10261 agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga tagaattcag ctcaagagtt
    10321 acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg tgagcataat cagttcagcg
    10381 tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa ctgacgataa
    10441 cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag acgctgttcc
    10501 tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg
    10561 cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt ttcaaccacg
    10621 tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat cggcaagcgg
    10681 atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat gcgctgccag
    10741 atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc
    10801 acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca atgtttcggg
    10861 agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt tattattttt
    10921 caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc gcacgaatgc
    10981 gtgcctccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc acgccatctt
    11041 catagagacc gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac
    11101 ccgggccatt caccacgcag ccaataatcg aaacgtccat cggagtgatg atatcttcca
    11161 ggcgttgctc cagcgcgtta actgtaccga taacatcaaa ttcctgacgc gaacaggtcg
    11221 gacaggcgat gaagttgata cctcgcgaac ggatacgaag cgatttcaaa atatcgaaac
    11281 cgactttgat ctcttcgacc ggatcggccg ccagtgatac gcgcagcgtg tcgccgatgc
    11341 cttcagacag cagcagacct aaaccaatgg cggattttac tgccccgctg cgcgcgccac
    11401 ccgcttcggt gatccccaga tgcagcggct gatcgatttg ttttgccagc aaacgataag
    11461 actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga tcgaagttca
    11521 ggcgatcgag atgatcaaca tggcgcatgg cggattccag caacgcctgc ggcgtcggtt
    11581 cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca atacggatcg
    11641 gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca ttaccgatat
    11701 tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc gcaatgcgat
    11761 agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg agtttgaacg
    11821 cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca acgcgttcca
    11881 gcgccttgat ttgattgacc gttgcttcga catctgtcgt acgcgtattg gtcatggact
    11941 gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata cgtgttgatt
    12001 ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac ccgtctgtta
    12061 ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag ttctgataaa
    12121 acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg gcgcaccaat
    12181 tttcagtttg tatggtgcct ggccggttaa gttcaaatta ccgtctttac gctgcatacc
    12241 gctaaacaat tttttaccgg tagcatcagt gacctccagc cagcaatcgg cagtaaagtt
    12301 catcaccagc gcattcggat cagccaccgg cgtggtcacg ccagcctgat cggttggcaa
    12361 cggcgcagca ccatctggcg ttgttgctgc cgttggtgcc ggggtcgcgg cggtatcaac
    12421 atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc
    12481 agttacggca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg gcggcgtgga
    12541 cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc cctgctcgct
    12601 attactgctc agttccgccg aagattgatc ggccatagtg gtgatctctt cctgctgagc
    12661 tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca acaccagcca
    12721 ggtgaaggtc atcagccagc cgtcgcgttt tttgcggcgt ttaccgaggg aaaaactctg
    12781 catcggcgca acttttgcag cccgtagtgg agcctgcttt tccagccctg gcagcagttc
    12841 ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca ggaatgttga
    12901 agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca
    12961 aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat tacgcaggcg
    13021 agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca ttcgctgcag
    13081 gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac accgcgaaac
    13141 atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc
    13201 cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa gtcagcaaaa
    13261 acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata aaacattacg
    13321 ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat gtcgatggct
    13381 tcaccctgca tccgtttacg cagtgtacgt ttcgtacggt cgataacatc gcccgccaac
    13441 tgaccacagg cagcatcgat atcatcacca cgcgttttac gcacaatagt ggtgaaaccg
    13501 tagctcatca gcacttttga gaaacggtcg atacggctgt tcgagctgcg tccatacggc
    13561 gcacccggga acgggttcca cgggatcagg ttgatcttac acggcgtatc tttcagcagt
    13621 tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca
    13681 atagtgactc ggccctgatt ggcgttggat ttctccagat aacggcgcac cgcagcaagg
    13741 aacgtttcga tattgtactt tttgttgatc ggcacaattt catcacgaat ttcgtcgttc
    13801 ggcgcgtgta aggaaattgc cagtgcaacg tcgatcatat cgcccagttt atccagcgcc
    13861 ggaactacac cggaagtgga aagcgtgacg cgacgtttag acagaccaaa accgaaatca
    13921 tcaagcatga tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg
    13981 cccatcatca ctacgttagt gatcgggcgc tgaccggtga cttttgctgc gccgacgatt
    14041 ttcgccgcac gccacacctg gccgataatt tccgacaccc gcaggttgcg gttaaagccc
    14101 tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga agagacgcag
    14161 agcgtggcac ggtcgtcttc agggatatac accgtttcga cgcgctgatc gcctacggcg
    14221 atcgcccatt taatggtgcc gtcagatgaa cgctgttctt caaccacttc cggtgcgcgg
    14281 atctccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt catctcatca
    14341 aaattgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg gaagggtttt
    14401 tcacctaaat ctttaaaaaa ctcccgcatc tgctgacgat tgagatccag caggttgatt
    14461 tttccatctt tggtggtgac gttttcaggt gtgactaatt gttcagacat atgctattcc
    14521 ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc cccggtgagc
    14581 ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc cacgtttgca
    14641 cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg ccgaagaaat
    14701 aagcgatttc gcgagcggca gattcgacgg agtcagaacc gtgggtacca ttttcggtca
    14761 ggctgtcagc gtaatcagcg cgcagagtac cggccagtgc gtttgccgga ttggtcgcgc
    14821 ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa accacgatcg
    14881 ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg tgttcagcat
    14941 aaaagccacg agcctgttca acggtcaggt gcagcatttt ggtgccgaca attttgaacc
    15001 ctgcagcttc aaagcgcgca aagatattac caatgacgtt ttttgctacc gcgttcggtt
    15061 tgataatgga aaaagtacgt tcaatagcca tttttacctc tgtaaagtat tcgttgttgt
    15121 ctgtacctgc gtacgagatg gcgcggatta taaagagcaa cagggccgtt gactattgat
    15181 gaaggtaaca tttttttaaa ataaaatgag ttttagcaac aactcgactt ttatggtgct
    15241 ctcatcccat agcgatgacg ttggcaataa agacaatttt gcgcattttg ctgaaaagta
    15301 agaaaggatt tttgacaggt tttacagact ttgcgattag catgaagatt cacaattttt
    15361 gccggtagaa tcgtcaattc aacggtcatt gcctgatgaa aacaaacagc aatacaattt
    15421 tggcatccgt tacacaaggc atcatttatt tgtaaatggc cttcgttaag cgaaaagacc
    15481 tgttgctcgc aggcccgcca gcatgcaccg caaagctgac acttttgagg atccatcatc
    15541 gggcgagcat cactaatctg gggccataaa taatgtaacc gacgtaatcc tggttgtatt
    15601 tgtgtcatct gaacatccgc acgctgcaca tggaacaaac gacgccggga aacattcacc
    15661 tcactggtat gtggagatac cacagaccag ccttcctcgc catattgccg taagcgttcg
    15721 tttaacagca ataatgcgcg ctcccatgcc ttcgcctgac tcagatcaat cgcgatcgct
    15781 gtgatcccct gctctttatg ccatagcaaa agttcttcca gcgtcggcgc aatagtggtg
    15841 aaaggtgcga ccagggtgtg attgcgaaaa taacgtaaag gggaattaac gccacgaata
    15901 gcctcttgtg gacaaataaa ctggcaaacc gcacactcaa tacattgttc cgcgtcgacc
    15961 gctatgccct cttctgtcag cgttagcgcc tgtgccgggc aagcctcgac acaggcatga
    16021 catgttgcga aacgaaagcg ttggcgcgcg cattccgcgc caatcgttat gttatgactt
    16081 aacattagcc gatactaaaa aagacaaaac gcagcataac ttcactcccc actagcatta
    16141 cgctaccgaa taacgccgcc gggatattct ttttacaacc accgacagca aagaacatca
    16201 tcccaatgac ggaaattccc cagcttagtg tcagtatggg ttttaactgt cccagcatcg
    16261 ctaatggcgc atgtggcaat gtggctatct gcatcgcatt tgccgttaaa tcccctatcc
    16321 acaacggctg aaccaacaaa cgcagcagaa ccaggagagc gatcaccaac acgcatccat
    16381 tacgcagggc atcaatctgc ggcaaaattc cgctaatacg aaacactgct accagcgctg
    16441 aaccgagaat tcccactgta cctaagaaca taatcagcgt gttgtaatgc tgccaggtga
    16501 taatcgacgt atggatgtaa acttgcgcca tgcagaaaac atcaacaatc ccgaccactg
    16561 ccgctagcat aagcaggagt tgccagccgc ctttgcgtaa catgaccagt aatgtggata
    16621 aacccaatag cgccagatac agcgcaccaa agataatttc ccgactcagc catgaactgg
    16681 aaaagtgact tagcgcatgc atcgcattaa gtggataacc caggtgtaag gtagaaatca
    16741 gtaaccctac ggcccctgcc aaaaaagcca acagtaatgg cggccgcata accggggggg
    16801 cgctggaggt gattaaaata tgacgatgaa gaaacgccag ccacaacgtc ataccgaccg
    16861 atgcctgtaa caacagcgta aaagccatta acggtaattc atgcatgatg tgacgcctcc
    16921 ttttccgcgc cctgatgcgg tttaatcacc agatttggct gcgtaatcga cgaatcaggt
    16981 aaacctttca catcactgac atcgccatac ttttcacgca actcatcaat agggccgaac
    17041 ttaatcgccc ccaacgggca cgttgccacg caaatgggtt gttctccttt agcttgcaga
    17101 tcaatacaga aatcgcattt cgacatctgc ccggtctgct cattcatttg tggtgcgccg
    17161 taaggacagg accaagcgca gtaaccgcaa ccgacgcact tatcagtatt cacgcgcaca
    17221 atgccgtctc cgggacgctt atgcatcgcc atcgtcgggc aatttttggt acagatcgga
    17281 tccgcgcaat ggttacagga aacagaaagg gtataagcaa agacattatg actcacaccg
    17341 ccctctccag tggcgatgaa gccacctccg ttcacttcat acacgcggcg aaaacgtcgc
    17401 ccaacctcaa ggttgttttt gtctttacag gccacctgac acgctttaca gcccgaacaa
    17461 cgcgaagagt caataaagaa ccctaattgt ttgtcgctga cagatgcaaa ttctttaaat
    17521 tggctcatgc tttctcaacc tccaccagca ttgtttgatg cgaattccct ttcgccagcg
    17581 ccgtaatgcg ggtcgaactc agtacattgg cacagcctcc gcgatcgata ccttgtgcat
    17641 ccggttgcca ccatgccccc gcctgcatag caactacgcc agggatgata cggggggtta
    17701 tttccaccgg gataatgctc acaccacgat cgttataaat ttttacgcta tctccttcga
    17761 caatcccgcg ctgttttgca tcttgcggat tcagccagag tttttgcgtc tgaacttctt
    17821 gcagccacgg gttagcatac tgggtggaat tagcacggtt tttccctttc caggtgatga
    17881 gctgcagcgg gtatttcgcc gtcagtttat cttctggccc ttcaaaggca gggacatagt
    17941 gcgaaagcgc gggaatttct ggatcttgca tatcaaaaag acgcttagag aagatctcaa
    18001 ttttgccgga aggcgtcggg aaaggattat tctgcggatc gcgaatattt gcttcgaaag
    18061 cgatgtgcgg cgcgcttttg aaaagatgtc gacgtgtttt ttgcagggtc gcgaagtccg
    18121 gcaaattttc atccggcatc gccagacgag tttgctccca gatatgctcg atccattgct
    18181 tctcatcacg tccttgactg aactcggctt cgacacccag ttttttcgcc acgtcacgca
    18241 accagtcata atcggtgcga cgttcaaaat ccggctcaat cagtttttct gacagaataa
    18301 gatagctcgc agttccccat gtttcgccga tattccagcg ttccataaag ctggtttccg
    18361 gcagcaacac atcggcatat ttcgcgctcg gagtcatgaa cagatcgctg agtacgatga
    18421 attctatttt agattcgtct tcaaggagct tcgcggcctg gtggacgtcc gggttctggt
    18481 tcgccagata attacctgcc agtgagaaca gcaggcggat attactgtcg agtttatcga
    18541 cacctttaag cccatcttgc ggcgtaactt tagaagcatc atccgccgcc tgcatccagt
    18601 tcataatcga gattttcgct tgtaccggat tttctggcat atctggtcca acgcagaatt
    18661 tacgattaga actaccgccg taacctgccg cccagccacc tttgatgccg acgttaccgg
    18721 taatcgaggc cagtaaagtt gaacctcggg cggtgcgttc gccgcaagta tgtcgctgtg
    18781 gcccccatcc ctgaatcaac gctgcgggtt ttgtagtggc gtaatcgcgt gccagttgac
    18841 gaatggattg tgctggaacg tgggtaattt tttctgccca ttccggcgtt ttgtgtatcc
    18901 catcttttgc gccaaaaagg taggcaacca acgattcgtt ggctggtacg ccttctggca
    18961 tagagttttc atcaaaaccg agagtatatg tgtcgataaa tgtcttatcg tggagattct
    19021 cgctgataat gacgtacatc atcgcgtcca tcaacgcatt gtccgtggtc ggcagcagcg
    19081 ggatccactg atcggcaagc gaagatacag tgtcggaata acgcggatct accacgataa
    19141 aacgcgtacc gttttgcttc attttttgaa aaaagtaatt ggtgtgaccg aagatggttt
    19201 ccgttgggtt atgcccccag aggatcacca gtttggtatc cagaagagta tccatcgagt
    19261 taccgctggc cgccacgcca tacgtgtaag gcgttgccgc cgcagtatta cccagactaa
    19321 ctgagtgata atattcaaga taaccaccgg ttaaatttaa cagccgacgc gccatcgcat
    19381 cgccagaaaa tgttccacca gaaacagccg tcccgacatg gacataacgt gaagccgggc
    19441 catattgttg cgtgatcctt ttcaggttat cggcaattaa ggtcgtcgcc tcatcccatg
    19501 aaatacgctc aaatttaccc tcgcctcgtt tacctacccg cttcatcgga tatttcaaac
    19561 ggtctggatg ataaacaaat ttacgatagc cgcgaccacg aacacaggcg cgcataatcg
    19621 gcatttgtgg atccagttcg ttatcgggaa gcgtggtaat ttgcgtcacc acaccgtcgc
    19681 gcatatgcgc ccgaatatcg cacttaccac cgcagtcaaa tgtgctacag gtgggcacga
    19741 ctttatcagc caccggagct gtaacttctg gcgacgcatc tggggattgc gcctttgcgg
    19801 tagaagaaat aaaaggcaaa gtgataagcg cggaagtcgc ttgtaaaaaa ctacgtcgtg
    19861 atacttgcgg aaatgtaatg cgttcctttt cgtctggcgt atccatcttg cgttcccgaa
    19921 aatgaaaaaa taaatattca cgtatttatt cgagaacaat gctgataaca atcaaataat
    19981 tagtcgttta aaaaaacgtg agattattaa attattaatg cattccaaat ataaatacca
    20041 taattagatt aataaatatt ttgtatattt cactgaaatg aaaaatgtac tgacgcgatt
    20101 tgccctacgt catccatcac cagtaattga taatcacctt tatccgtcaa atgcagggtc
    20161 acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg ttcacctgcc
    20221 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
    20281 tcgccatcgc gaacgcctgt cagctgcagc gggagctggg catcgtgacc gtatggcggg
    20341 caaattgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg caaccagggt
    20401 tccagcggta gcggccagac atttatcatt tcttgttgcg cctgagggca atcagcggca
    20461 acacgtttgc cattttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc
    20521 tccggcagta atagagttgg cggctgactc ccgtccagca gccaggttgc caggcggcgg
    20581 cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcaaat aacgccacga
    20641 gtgactgagt tcgggcgcgg gtcttccggc agattagcac tgcgcgacag taagatatta
    20701 ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    20761 ccgtccggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    20821 gcgtcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    20881 gcaccatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgcagcc
    20941 tccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg caatttgcct
    21001 gccttgccct ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcacca
    21061 gcgccgccga gaatgagcga aagattcggc gccgcaccgt tgggcaaata taacggcaat
    21121 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag tacctgcaca
    21181 gcaggtaaat tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc atgaaaaccg
    21241 ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg caacagcgat
    21301 gccggatgga tcaagccttc atccagcgcc agaccgtaaa caaacggttt gagcaccgac
    21361 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    21421 tcaaccgaac ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    21481 agtgagctgc gcggtggtaa tcgtcctttc cagttttgcg ccagttcttc cagacgtcgt
    21541 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    21601 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    21661 cttgactctt ttacctgctc acgggaccac acaccttgca cggccatccg ttcgagcact
    21721 ttattacgcg cgacttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct
    21781 tgcggcaaaa ccgccagcat tgccgcctcg gaatagctta aattcgcagg cgactttccg
    21841 agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg agcgcggtta
    21901 agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa cgcgcgccag
    21961 agctggcgaa ttttgccgcc aaatgttttg gggtgaggat caagcagacg agcaacctgc
    22021 atagtgagcg tgctgccacc ggaaataacc cgtcccgaag tgagatcttg ccatgctgcg
    22081 cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    22141 atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg
    22201 ccgtcagcat cggcgaagcg ccagagcggc gtaccatcct gcgccacgac cactcgtgcg
    22261 ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc tgcaagaaaa
    22321 aggagaaatg gcgcagctgc cagcgttatc cagcagccgc gtttggttaa caagcgaggc
    22381 atttacggtc tgacaatcag caaatcttca gccgcgccgg tcgcccgcca ttggggaaca
    22441 tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc
    22501 agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac aaagcgatcg
    22561 tcacggaact caatgtgctt aatactcgcc tgctgcatct ggttcagtaa gttctgcact
    22621 tcgccaccac tttgctccag gctggcgcta ccgttcgcca gattctggtt ttccagttcc
    22681 agacccgcag gcagcagatc cacgactaac gcatccggca cgctgttact ggcttttacc
    22741 tgcaaccaga ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttacca
    22801 tcagtaccaa gaatatgacg ctcaatttgc agcacattgt tcgccggtaa aggtgcggat
    22861 tgcggataac cgctggcatc cacacgcagc cataacggct gctcgccact gttggtcacc
    22921 tgcaaggtgg caagttgatc gctattcaga ttgctgtttt gcgctttctc gcctgtcagc
    22981 ggctcagttg agaaagaggt ttgcgcctgc catttacctg gtaaatcctg aatcgtacgg
    23041 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc aaacgcctgc
    23101 tgcgaaagtg tgttcagcag ggagtactgc tcatcgggta gcagtttgtt ctcttccagc
    23161 aaggagagca tcaacgcgtt gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    23221 tcatcactat tacgcggcgt ttttagcgcc agcgcaatcg cttcttcaca acgcgtcgca
    23281 tcacccatgg ttttcagcgc aacgccaagt tgcagcagcg gtaaaccaga agcggcatct
    23341 gcacgatgct cccagatttc acgcagcgca cccagcggag ccttttgctg acgggccaac
    23401 accagcgcag cgtatgactg tacggcgaat ttactggctt tgagattatc cgcgtacggg
    23461 atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    23521 gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    23581 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    23641 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    23701 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag tgacgggaac
    23761 aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc tttgatataa
    23821 cgtgcgatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggt
    23881 gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg taacgccgta
    23941 ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca ctgcttatgc
    24001 tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg aatttctcca
    24061 tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac acctggcgct
    24121 aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc ggtcagggca
    24181 acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag acgcgaggta
    24241 tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc ggcaactatc
    24301 actttgcttt cgttgctacc gaagtcatct gccgtccagg cttgcgccat gacgcgcaat
    24361 tcaccgttaa aatcgccaat cggcagtgta accgagcctt cgccctgttc gttgagcgtt
    24421 accggcagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt
    24481 ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg accttcaata
    24541 acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcctgc
    24601 cacggatctg gcgtgacgta gtcagtaata ttcagcacac cactatcaac ggcagacacc
    24661 agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat tttcacggtt
    24721 aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag gcgacggttt
    24781 tcatcaccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc ggagcgagat
    24841 ttatcgccag gacgcaccac cagcgtactc aaataaagat catgacgatt ccaggtttta
    24901 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc
    24961 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcaatatgc
    25021 aacttaatgg tatcgccagg gcgataactg gctttatcta gtttcagcgt gacacggtcg
    25081 ggtcgcacgg caccgctccc gtcgctgttg tcctgccagc tatagccagc ccagaaacgc
    25141 acactactga ccgcttcatt cggcgctttg acttccagac gataagcgcc ccactctacc
    25201 gggaaactga ccttgccggt ttcgtccgct ttcagatcca gagtttgttc attttcaatc
    25261 agatcttttt gatcaaactg tgattgccag ccttcatctt ctgaccagtt ccagtagtaa
    25321 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgc
    25381 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    25441 accgtgctgt cagtgcgata atcgtaaacc gatttcgagg cgaactgcgg acggatcccc
    25501 ggcaatgcat cggcaggcca gatagcctgc tcagcgcgac gcgtcactgg gcgaccgccc
    25561 gattccagca aactgccctg gaaaataacc tgtaatgggg aatgcgtttc cttccactgg
    25621 ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc
    25681 gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca
    25741 gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggtgca
    25801 ccgtacaggt agtatcccac cacggagaat ttcacttcat cattcggcgt aagcggggtt
    25861 ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac gtggaaatcc
    25921 cacatccgat actggttatc gcccgtgtta gcgcgaatat gccacatacc ggttgccgca
    25981 ttgctatcga gtggccaggt aaagtggtag aggccatttt ccggctgact aacgacgctc
    26041 ctgagtacct gcccatcggg tttaatcact tctaacttga tgggttgatt gggcaacgct
    26101 ttaccgtctg catcacgcag caaaccattg aggatgaccg tttcacccgg gcgataaaga
    26161 tcgcgcgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    26221 tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgt
    26281 gccagcaata atgcggcgtt tttatcattt tccagctgca catgcccctg agcgtcactg
    26341 gttgcctgag tcagagtctg ccctttctca tttaataaag agacttcaat tccttgctgg
    26401 gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    26461 gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    26521 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    26581 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    26641 accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga attgcggtat
    26701 tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa gttaacatcg
    26761 acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttacc aggcagcagc
    26821 gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat agttttttcg
    26881 taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat agtaacgatc
    26941 aaatcacgtt tcggttcgag gtggcgtaaa cgcagctctt taagattatc tgacagctcc
    27001 caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg tgagaaatcc
    27061 tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc atcaagttgg
    27121 acttcagaga gatccagcaa tgtcagcgcc ttaccggcac tctgttgtgc cagtttttgt
    27181 cgctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc tttagtaact
    27241 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    27301 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac
    27361 aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca
    27421 gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc
    27481 ctcttttgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat
    27541 ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga
    27601 tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg aagatcgtaa
    27661 cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg atatcgaagc
    27721 gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt
    27781 ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa
    27841 tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc
    27901 gattctgggg ggtggacttg caggctggca gcgcgatgat ctgttgttag aagaaggtgc
    27961 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagcag tggtgaaagt
    28021 aaccgatgtg ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc
    28081 tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
    28141 cggtgcattg aacgttccgt ggacggaact ggtgcgcgaa ggcgaactaa aaacgaccga
    28201 tgaactggat gcgatatttt ttggtcgcgg cgtcagctat gacaaaccga ttatcgtcag
    28261 ctgcggctct ggtgtaacag cagccgtggt tttgttggca ctcgcgacgc tggatgtgcc
    28321 aaacgtaaaa ttatacgacg gtgcgtggag tgaatggggc gcacgggcag atttaccggt
    28381 tgagccattg aaataagctt tatacaggca ataaaaaacc gccgaaaata aaaaaccgcc
    28441 gaatttggcg gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgacttgtg
    28501 ttgccggagc aacgcaggca ccccacagtg ttgaaatact ctcttttatg cctggctgtg
    28561 tcaagataat aaacagagta attattcaaa cataagagta ttattcttaa tggccctttt
    28621 cgccgtctcg caaacgggcg ctggctttag gaaaggatgt tccatgaccg taaatacagg
    28681 caccccacag tgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt
    28741 cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg
    28801 ctgttaaccg aggtaaataa taacctgagt ctctccggca acaatttact ggtggttaac
    28861 aaccttcaga acagcaggta agcccgaatg ccgccctttg gggcggcata ttttagatta
    28921 tccggttctg tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcgcaa
    28981 tatgttcgat attaaaatga cctggcttat tagtccgaat tcagacaaat ataaacaaat
    29041 cccgctcaat attaaaaata ctaaccggta aaagatatta cttaaacatg taaattcact
    29101 ttctttaagc aacaaaaaac cgccaaaatc aggcggttat ttgttgccgg tttggttcgc
    29161 ggcctctcca gcagattgac ttgtgttgca tgagcaacgc aggtgcttca cagcagagca
    29221 atattcgcca gcaactctct ttttgtcaag caaaagagag taattattgt ttatttagcg
    29281 tattatcgac accggccctt tccgccgtct cgcaaacggg cgctggcttt aggaaaggat
    29341 gttccgtggc cgtaaatgca ggtgtttcac agcgcttgct atcgcggcaa tatcgccagt
    29401 ggtgctgtcg tgatgcggtc ttcgcatgga ccgcacaatg aagatacggt gcttttgtat
    29461 cgtacttatt gtttctggtg cgctgttaac cgaggtaaat aataacctga gtctctccgg
    29521 caacaattta ctggtggtta acaaccttca gaacagcagg taagcccgaa tgccgccctt
    29581 tggggcggca tattttagat tatccggttc tgtttaaagt cacgcaaaaa accaccccaa
    29641 cgacgttcat agaatggcgc aatatgttcg gtaataaagt ggctaattcc tttttcccct
    29701 tttttcacct gacaaatatc gattggttca tcggcaggta atgtatcggt cgctacactt
    29761 cccgccgcct gaataatttc ttcgacatca ccatcggctt caatgccaat aagtaaatta
    29821 ggctgtgcct cttcgttctc tttaattgaa catataaaag cacgcttcac cggcttaata
    29881 gttttaaata aggtggtgag tgaatcaatc atttgtgctg gcggctctgc gacttccgat
    29941 aatatcagcg attcaccgcc ttccaggatt tcctggctgc tcagcggatt tccctcttca
    30001 ccaatcaaca aactgatttc acgcggcata aattctttac cggtcggcag tttggcatta
    30061 aggaagagcg tttcgccaag ggtcatctca aacaatgtgc gaacgggcat tacgacaaat
    30121 gcctgttcgt cttcaaccgc ctgttgaagt gcttctaacg aggtgaaaaa aggaataacg
    30181 ctggtgccgt cttctttttc ccagtgctgt aaatcaagcg cgctatcttc aaccacagcc
    30241 tcgccctgcg ccgccgtacc aggcacccag acggtggatt ccagtagagt acggaaaaag
    30301 gccgggcggt gcgccggttc ggttgctgct ttttccagca ggtcttcaag ttcatttttt
    30361 gtttcggaca taagaaccac aattcattca acgttcgggg cacaagtgcc tgatgcgcca
    30421 tacttatcag gcctacaagg gagtcgcaat gtattgaatt tgcaatactt tgtaggccgg
    30481 ataaggcgtt tacgccgcat ccggcatatt agtttacgcc gttaacagat tagcaatcgt
    30541 gcgcacacca agtcccgtag cacccgcaga ccactgttca accggcgctt tacggtaagt
    30601 cgccgagcag tcgatatgca gccagccttg ctgatagttc tcaacaaagt gcgacaggaa
    30661 gcccgccgcc gtgctcgcgc ctgccggata cgccgcgctt ccggtattgt tcagttcggc
    30721 aaagttagac ggcagctggc tgcggtggaa ctccgccagc ggcagacgcc agaatggttc
    30781 gttctcttgt gaggcactcg ccagcaaacg accggccagc gcatcgtcaa aactgaacag
    30841 cgcatgataa tcattaccca gcgcggtttt cgccgccccg gtgagggtcg ccgcatcaat
    30901 gatcagttcc ggtttctgcg cactggcatc aatcaggcca tcggccagca ccagacgccc
    30961 ttcggcatca gtgttcatca cttcaacttt tttaccgttg cgataggtga tgatatcgcc
    31021 cagcttgaac gcattgccgc taatcaggtt atccgcacag cagaggaaca gcttcacgcg
    31081 cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc
    31141 catgtccgac ttcatcgagt ccataaacgc agtctgtttg atgctgtagc cgccggagtc
    31201 aaaagtgata cctttaccta ccaggcacgc gaacactggc gcttctttat cgccagttgg
    31261 gttgtaatcc agcgccagca ataccggaga acgttctgaa ccgcgtccga cggtgtgcag
    31321 ccccatataa ccttgctcac gcagatcttc gcctttggtg atccgataag tcacacgatc
    31381 gcccgcgacg ttgctgatca gatcaacagc acgctgtgcc agttgcgatg gtcccaattc
    31441 ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc ggttatccag
    31501 ttcctggcgc tgggcatcgt ccagatccgg ccactccact ttacgcgtgc ctttcggggc
    31561 tttgtaacct tgccagaatg cccagcagcg atccgcatcc cagccttcac cgcttagctg
    31621 aacatgcttg atgcccagac cgtcaatctt gcgcgccgca cgctggatca gccccagatc
    31681 gtctgccccg ttcaaatgca gagtaatgcc atcattatta atgctgtaag ttgctttttc
    31741 tccccagcgc gcgtcggcag gttgggtaga gagggtaatt ttcatcgctt ctgtcatttt
    31801 agttatcctt attcgtttaa cagcaaaacg ggctaccccc aggcagcccg ttcattcact
    31861 cggcttcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg gatgcctgcg
    31921 ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg aatcgaaccg
    31981 ttttcggatc aagatcggga tacgcatcgt acagtgcttc gccaatttcg cggctatcgg
    32041 tccacttaag tcccatacta acctctgtta atgctcacgc gcatggttga tagtgtaacg
    32101 cgggatttcg accactaaat cttcgtcggt aacgcgcgcc tggcagctta aacggctttc
    32161 cggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc tttccggcag
    32221 cgagtcaaaa ccttcgcgaa cgatgcagtg gcaggtggtg caagcacagg atttttcaca
    32281 ggcgtgttca atctcgatac cgttacgcag agctgcgtcg agaatggttt caccgctatt
    32341 agcttccaga acagcgccat cagggcagag ctcctgatga ggcaaaataa caatctttgg
    32401 catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac tggtccatgc
    32461 ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt tcgatggcgt
    32521 caacatcatc gccctgcgcc acttcactca ggtgagcggc agcatcgtca atgacctgac
    32581 gttctgcggc gcttaacagc gcggcatcag cagccagcgc gccgtgcaga ctttccagca
    32641 cacgcgccgc ttctactttt tgttctgcca gcattcgggc ttttacgtcc tgctcggcat
    32701 agctcattga gtctttgatc atcgaagcga tttcgctatc ggtcagaccg taagacggtt
    32761 tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg ctcaaaagac
    32821 cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt agcgccggaa
    32881 taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg cgctcaccct
    32941 gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa tcctgagcgc
    33001 gggccaccgg aatagtggta ttacgcggaa tcactttctc caccaggccg cccatcgttt
    33061 cgaggcccag cgacagtggg atcacatcaa gcaacagcat ttcgctgtct ggcttgttac
    33121 ccaccagaat atccgcctga atcgccgcgc caatagcgac gactttatcc gggtcgatgg
    33181 aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc ggcacgcgag
    33241 tagaaccgcc caccatcacc acttccagca cttcatcagc ttctacaccc gcgtctttca
    33301 gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt tcattgaatt
    33361 gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag tccgcatcgc
    33421 tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg cggttatcgc
    33481 tacgatcagg aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg tcgaaatcat
    33541 cgccgccgag cgcggaatca ccgccggttg ccagcacttc aaacacgccg cgacttaagc
    33601 gcagaatgga aatatcaaac gtcccgccac cgaggtcata aacggcgatc acgccttcct
    33661 gaccggaatc cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt aagcgcagga
    33721 cgtgaaggcc cgccagacgc gccgcgtctt tggtgccctg acgctgggca tcgtcaaagt
    33781 acgccggaac ggtgataacg acaccatcca gctcgcctgc cagggcttca gttgcccgcg
    33841 ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc cccgccgccg
    33901 tttcaatcat cggcaggccg ttttcgctgg cctggaattg ataaggcaga tgcggatagc
    33961 gttgctggat atcagccagc gagcgtccca tcaggcgttt aacagaacta attgtgttgg
    34021 cggtatcgag cgctgcatta gtacgcgcgt cataacccac cgaatgccct tgctgttgat
    34081 agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt tcggcctgac
    34141 cgctgcgcac tgtcgccacc agcgagttgg ttgtgcccag gtcaataccg gccgccagac
    34201 gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag gccatgttta
    34261 gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact gcttcgcagt
    34321 ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccaggt ctcgttgtct
    34381 aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg tttgataaag
    34441 ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagctcttc gcgcagctcc
    34501 aactgttcca tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc gagatcaaag
    34561 ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt ttgccaggcc
    34621 tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct tccgctggcg
    34681 aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag cgcctgggta
    34741 tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac attctcaggg
    34801 ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc aggcgaccac
    34861 ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca cactcatctt
    34921 taacgttcgg gttggtgaat ttaaaccctt cgttcaggcc ttcttttacg aagtccagct
    34981 gcgtaccgtc cagaaattgc aggcttttgc catcgaccac gactttcacg cctttgtctt
    35041 caaacacgat gtcttccggc gtcggttcgt caacaaattc cagtacataa gccatacctg
    35101 aacatccgga ggttctcacg cccagacgca ggccaaaccc tttgccgcgg ttagccagaa
    35161 aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc aaacctcaac
    35221 tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat cgcgtcttct
    35281 gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc aatatcggtg
    35341 tttttgatcg cctgcgcttc gtcgagagac ttccctttca cccattcggt gaccagggag
    35401 ctggaagcga tagcggaacc gcagccgtaa gttttaaaac gcgcgtcttc aatgatacct
    35461 tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc acccaccatg
    35521 ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg attctcgtaa
    35581 tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga taccgattaa
    35641 tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc ccacagcgga
    35701 gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta gtcgatctct
    35761 tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag ctcgtcgttc
    35821 agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca ggcggagccg
    35881 gaagaaaccg cgaggtcttt cagcgccata atcagcgact caccttcaac gtagttgaag
    35941 ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta aacttcttcg
    36001 atatctttga tgccgttcca cagacggtta cgcagtccgc gcagacgttc catctcggtc
    36061 gccatctctt cttttgcgat gcgataggct tcgcccattc cgacgatctg gtgaacaggc
    36121 agagtgccgg aacgcatacc gcgttcgtga ccgccgccgt gcatttgcgc ttcgatgcgt
    36181 acgcgcggtt tacgacgcac atacagcgca ccaatacctt tcgggccata gattttgtga
    36241 ccggagaaag acatcaggtc aactttcaac tggctcagat cgataggcag tttgcccacg
    36301 ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc gccgatagcc
    36361 gcgatatcct gcaccacgcc gatttcgtta ttcacgtgca tgatagaaac gaggatggtg
    36421 tcgtcacgca tcgccgcttc gagttctttc aggtcgataa taccgttacg ctgcggtgcc
    36481 aggtaggtga cttcgaaacc ttcgcgctcc agctgacggc aggtatccag taccgctttg
    36541 tgttcggttt tgctggtgat gatgtgcttg ccttttttct gataaaagtt ggctgcacct
    36601 ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc acgcggatca
    36661 gcgccgacca gatcggcaat ctgattacgg gcgatatcta ccgcttcttc agcctgccag
    36721 ccgaaacggt gagaacggga ggccgggtta ccaaaggttc cgtccatcgt cataaactgc
    36781 atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc gagataaatc
    36841 ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaatc aggctaccgg
    36901 ctggatgtac gaccgtgttt acggagtatt tagcactccg gcctgattct gaattctttt
    36961 tattaagcgc gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg cgcgtcgtga
    37021 gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc gcctaaagta
    37081 atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc gtgggtcagg
    37141 catttatccc cgccctggca gccgccttta ccctgacaac gggtagcatc tacagattcg
    37201 tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc taacagataa
    37261 ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg ggaaaacagt
    37321 tgttccagat aagaaaggga aattccctga cgttcggaaa tatcagccaa cggtaccggg
    37381 cccgcttcag agttgagcgc aacgtcaagc attgcggtca cggcatagcg ccctttagat
    37441 gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt ttattgtaaa
    37501 agtgggggta ttgcatagca gggtcaagtc tgacattccc gagtaaattg gtcaactatt
    37561 tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc gtggcgtttg
    37621 tacctgttgt cggatgcggt gtgaacacct tgtccgacct gggattccgt gcacttacag
    37681 gcctgataag acgcgttaag catcgcatca ggcaaccact gttattcggc cttattacct
    37741 ttattctgct gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc
    37801 gggcgcgcac gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca
    37861 cggataaaac cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca
    37921 tcgaccagcg gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc
    37981 agccaggcca tacgcacttc ataggcaata acctgaaccg ccattgccag gttcagcgaa
    38041 ctgtattccg ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg
    38101 gtcaagccga cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg
    38161 acacttttca gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt
    38221 gcgctggtgc ctaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg
    38281 tgagcattac caatcacatc gctggccccc gccgccaggg caatagcctg ggagtcaggt
    38341 ttcaccagtg gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca
    38401 acagaaccca tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc
    38461 attgtctttc ttcgtctaaa gattattcgc gcatcttatc ataaaacgaa gacagatgcc
    38521 gatctcgctg ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg
    38581 atgcatccga tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc
    38641 aaaaactatg aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg
    38701 accaacgtag ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttaccca
    38761 cagcacacca tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa
    38821 tgggttatcg atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg
    38881 gtatctatcg ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg
    38941 cgtaacgaac tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg
    39001 cgcggcagca ccgctcgcga tctcgacggt actattctgg cgaccggctt cccgttcaaa
    39061 gcaaaacagt acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca
    39121 gacttccgtc gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt
    39181 gacggtttct ttgaaatcgg tctgcgcccg tgggacttcg ctgcaggcga gctgctggtt
    39241 cgtgaagcgg gcggcatcgt cagcgacttc accggtggtc ataactatat gctgaccggt
    39301 aacatcgttg ctggtaaccc gcgtgttgtt aaagccatgc tggcgaacat gcgtgacgag
    39361 ttaagcgacg ctctgaagcg ttaatgactc aggcgggtga tatcactcac ccgccctcgc
    39421 ctttcaggcg ctattccgaa atacttcctc accgctttac tttctttcgt cactctccca
    39481 ccatctttcc ccgattaatg gatgataaag aagtcgcaag gaaaggttat ggcactgcca
    39541 gtgaacaaac gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat
    39601 ttagtgccgc gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc
    39661 gacccctggt cggcggaatc cgtcgaattt acggctaaga acggtactcg tctgcaaggc
    39721 tggtttatcc cttcttcgac gggccctgct gacaacgcca tcgcaaccat cattcatgct
    39781 cacggcaatg ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt
    39841 aatttcaacg tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc
    39901 caggccggat tgctggacga tacgcaaagc gccatcaatg tggtgcgcca tcgcagtgat
    39961 gtaaacccac aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct
    40021 gttattggtc agggtgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc
    40081 tcttatgcaa ccatcgccaa ccagatgatc cccggcagtg gctacttact tgatgagagt
    40141 tacagcggcg aaaattacat cgccagcgtc agcccgatcc cgcttttact cattcacggt
    40201 aaagctgatc acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag
    40261 ccaaaacggt tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac
    40321 ggcgatgttt atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac
    40381 taacccatga tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca
    40441 ctggtcagaa cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat
    40501 tagcattccc ttcgccattt ccctgagcaa actttagcta ttcttatcaa ttatgcttat
    40561 gggagatcta cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg
    40621 ccgctgccag atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt
    40681 cagcgagctt aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg
    40741 ggagatcctg cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt
    40801 tgaaggtaca gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg
    40861 tctgtgtccg tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg
    40921 gcgcggaatt gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg
    40981 ctcccgacgg ctgcaaaaac gctttgaatc gcgcgatatt catttcctgt gtctgtatct
    41041 gcaatattgt ttgctgcagc accacgctgg aattacgcct cagtttaatc cgctccaacg
    41101 tcgctgggcg gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca
    41161 gcgtcgggcg ctccagcctg taccacctga tgagccactg tttatggcac tacttttttc
    41221 catgttgcgg gttcccgatc cattgcggga tgcgcatcag cgggacagac aattgcgtca
    41281 gtctatcaaa cgtctggtaa accattttcg tgagctggga aatgtttgtt tttatgatga
    41341 acaggggtta tgtgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt
    41401 tgccatcggt attgataata ccctgccgga agagttcgcc agactgtatc cacgcctggt
    41461 acgcaccacc cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga
    41521 tgaggaaagt ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct
    41581 gcatgaaaaa cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga
    41641 gcagcagtta cgcgaactaa cgttactgcc gctcaacatt aaacatatgt cggtaaaggc
    41701 atttttgcag acaggcgctc cgcgtggcgc ggcactgatt attgctcctt ataccatgcc
    41761 gttaccgctc ttttcaccac cgctgatcta tacggacctg acactgacaa cacatcaaca
    41821 ggagcagatc cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca
    41881 ttgccggaag cgccaccagc gccatcaccc agaacacgcc gtggcccaga tattgataca
    41941 ggaaaccggc gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa
    42001 ccgcctgtaa acggatgact tcgctaccct ggcgagcggc aatatagcgc atggcggcca
    42061 ggtggcagac cgtgaaggtg ccgcaatgca gaatttgcac cactatcaac cacggcaacg
    42121 ccgtagttgc tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca
    42181 tatcgcgtgc actacaacgg cggaaaagtt tattactcag tgcaaagata atgacttccg
    42241 ccaccacgcc cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc
    42301 agtaaatggc gctaaaaccg taataggccg catgcgcccc ctgcaataaa caaacgcagg
    42361 ccagaaagcg ccagttctgg cgaaccagcg ccaaccacgc tgaccaaccg gtgctctcct
    42421 gttggcggct tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg
    42481 atgccacgcc caacgtcaac agcacgagga tcacccgata atcaaacata gtgaccagtt
    42541 tgcccgtcag cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt
    42601 aatcaagcgg gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg
    42661 gtgagaaaaa gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg
    42721 cccagaaggc gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca
    42781 ggcgagaagg atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca
    42841 gacctgcccc caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga
    42901 cgctccagaa aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca
    42961 accagcgcgt ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggagtt
    43021 aatcgaatgt tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt
    43081 aacgccagtt cacaagcact caaaaactgc gatccgtgac gcatgttgca aagataatgt
    43141 gtaattatgc cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg
    43201 ccggcttcat ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg
    43261 caatgggacg aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag
    43321 tgacgcccag ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga
    43381 cgatatttgg ctggctgttt tcttgtgcaa accacgcttt aacgatcggc gcaagcgaac
    43441 cggaatacgc cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct
    43501 ctttttcatg tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa
    43561 gctccagata atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt
    43621 caccacggcg aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg
    43681 tgtctggctg tctgagacga aacatcggta atacttttgg cagtaaattc acttccgccg
    43741 atggcacaaa gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt
    43801 tggcattttc cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg
    43861 ccgtcaacgc gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa
    43921 gatcgcggat ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg
    43981 taaagttcag tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct
    44041 tccccttgtt atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca
    44101 accaaccagc aacatcctta tggcacaaaa atagaaggtc aatacatctt atctttcagg
    44161 attaaaaaat atgaccacac cctcagattt gaacatttac caactgattg acacccaaaa
    44221 cggtcgggtc actccgcgta tttataccga cccggacatt taccaactgg agcttgagcg
    44281 tattttcggt cgttgctggc tatttctcgc ccacgaaagc cagatcccaa aacccggtga
    44341 tttctttaac acctacatgg gagaagatgc agttgtcgtg gtgcgccaga aagacggcag
    44401 catcaaggcg tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg
    44461 cggcaacact cgcgccttta cctgcccgta tcacggctgg tcttatggca ttaacggcga
    44521 gttgatcgat gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg
    44581 ggggctaaac gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga
    44641 taccagcgca ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat
    44701 gctggatcgt cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa
    44761 ctgcaactgg aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag
    44821 ccatgcttct gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg
    44881 tgatggacaa accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca
    44941 ggacggtcac ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga
    45001 tggcgcagtt tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga
    45061 agttcgcgcc ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa
    45121 cggcactgcc acgctccgcg tctggcatcc gcgcggccct gatcaagtcg aagtgtgggc
    45181 gttctgtatt actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc
    45241 cactcgtgct tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga
    45301 aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg
    45361 gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc
    45421 agaaactgcc gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg
    45481 gcaggaagtg ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa
    45541 gtttcactag agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg
    45601 gacgactgga aatttcgtga ctggctggcg cagctcgacg aagagattcg ttacaccatg
    45661 cgcaccacgg ttaacgcgca aacgcgcgac cgccgcaaag gcgtccagcc gccgacgacc
    45721 tggattttta atgacactaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc
    45781 atggcctggg cagaagagcc accgtcacgc acccgtcact taatcagcaa ctgccagata
    45841 agcgaaaccg acatcccaaa cgtatttgct gttcgggtga attatctgct ttatcgggca
    45901 caaaaagagc gcgatgaaac gttctatgtc gggactcgtt ttgacaaagt tcgccgtctg
    45961 gaagatgaca actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact
    46021 tcccataacc tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg
    46081 tgccggaggg tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg
    46141 gcggcgagtt ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag
    46201 ggtatctgga agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga
    46261 aaacggggaa agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg
    46321 ttgaaggtgg tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa
    46381 cgagtccatt tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt
    46441 tatcgaagaa ggcgcgcagg ttgccacgct ggaactgtcg gcggcgaaag tcgccagtct
    46501 gcgtcagcga tttggtgaac atattctggc ggtggaaggc aacgtgacct gttatgccga
    46561 ttatcaacgc gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg
    46621 caatgcgggc atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga
    46681 aaccggcttc cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gtgcaaaagc
    46741 ctgcgctccg gcgttaatcg ccggtgaagg cagcatgatt ttcacactat caaatgccgc
    46801 ctggtatccc ggcggcggtg gcccgctgta caccgccagt aaacatgccg caatcgggct
    46861 tattcgccaa ctggcttatg aactggctcc gaaagtacgg gtgaatggcg tcggcccgtg
    46921 tggtatggcc agcgacctgc gcggcccaca ggcgctcggg caaagcgaaa cctcgataat
    46981 gcagtctctg acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc
    47041 ggcggatttt acgggtccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag
    47101 cggtgtgatg atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagctgc
    47161 tgggctggat ctttaaggaa gtacgatgaa agaaaaaacg atcattattg tcggtggtgg
    47221 gcaagcggcg gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca
    47281 tctgttttcc gatgagcaac atcttcctta tgaacgccct ccgctctcga aatccatgtt
    47341 gctggaagat tccccacagt tgcagtctgt gttacccgct cactggtggc aggaaaacaa
    47401 tgttcatctg cattccggtg taaccatcaa aacattgggc cgcgacacac gagagttagt
    47461 gttagccaac ggcgaaagct ggcactggga tcagcttttt atagcaaccg gcgcggcagc
    47521 tcgaccgctg ccgttgcttg atgcactggg agaacgctgc tttaccttgc gccatgccgg
    47581 tgatgccgcc agactgcgag aagttctgca gccagaacgg tcagtcgtaa ttgtcggtgc
    47641 cggaactatt ggtctggaac tggctgccag cgccacgcag cgcggatgta aggtgacagt
    47701 gattgaactg gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct
    47761 tttacagcgc catcaacagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt
    47821 ggtcgatggt gaaaaagtag aactgacgct gcaaagtggc gagacgcttc aggccgatgt
    47881 ggtgatttac ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga
    47941 tactaccaat ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc
    48001 cggtggcgat gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg
    48061 ggaaaacgcc aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgcttcc
    48121 gctactgccg ccgccgtggt tctggagtga tcagtacagt gataacttac agtttattgg
    48181 cgatatgcgt ggcgatgact ggctttgtcg tggcaacccg gaaacccaga aagcgatttg
    48241 gtttaatctg caaaacggcg tgcttatcgg tgcggtaacg ctgaatcagg gacgtgagat
    48301 tcgctcaatc cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac agctgacaga
    48361 tgagaacatc gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg
    48421 ggcgtggcga ggtaccagag tgtgtattac gtttaaatca cattatcttg caaagggatt
    48481 ggttatgaac tcactacgtt attttgattt tggtgctgcc cgccccgttt tgttattaat
    48541 tgcccgtatc gccgtggtcc taattttcat tatttttggt tttcccaaaa tgatgggctt
    48601 tgacggtacg gtccaatata tggcctcgct gggcgcaccg atgccgatgc tggcagcgat
    48661 tattgcggta gttatggaag tgcccgccgc gatattaatc gtgcttggct ttttcacccg
    48721 tccgctggcg gtactgttta ttttctacac gctgggtacg gcggtgattg gtcaccatta
    48781 ctgggatatg accggcgatg ccgtcgggcc aaatatgatt aatttctgga agaatgtcag
    48841 tatcgctggc gcgttcttgc tattggcaat taccgggccg ggggcaattt ctctcgatcg
    48901 ccgttaggaa agatgccgga tgcggcgtga acgccttatc cggcatttta taaattacag
    48961 ccactctacc cgcaacgaca tctctgaggt tgttgattcc cccggcgcaa gcgcaatgag
    49021 gtcaccccct tccgggcgat gatggtcatc cggcgcatgg ctcatcggct ccagacagaa
    49081 gaaatcaaac gcatatcctt tatcaaacgc cgggtcggaa acaaagatga aataacacgg
    49141 tgcaggtggc gtcgtttcta tgatgatggc ataaccctcc tgcggctgct cgatgcgggc
    49201 ctgaccattc catccggcaa aaccattgtt cacccactgg cgcggcaacg gcgctagctg
    49261 gttaaaatcc agttcttgcg gtagctgctc gcaaaactca cccgccagcc actgctcccg
    49321 ctccagccag taaccgctcg cctgtgcctg aatccgcgtt tgcggcgaca acggaaaata
    49381 aggatgccag ccggtaccaa atggcagcgt ctctgcccct tgattggtga cagagagcgt
    49441 caccgtcagc gtatccgccg ttaaatgaaa cgcctgactt acccgatagt gatagacacc
    49501 gctgcgatgt tcatacacca gacacaaact atcatcgctg tgcgagacac attgccactc
    49561 gcccagccag ccatcgccgt gtagatagtg cgcatcccac tcaacattcg gttgcagttg
    49621 atactcacgc ccctgccaga caaaatggtt gccgctcacc cggtttgcaa atggcactag
    49681 cggaaaacaa gaggcatcgg ttgccacacc gcttttttta ccaggacgta agagcggcgt
    49741 cgtatcacgc cagaaacctt cgatgacacc gccctggtca gagacatcca gctttagcgg
    49801 tccgtgagat aaggtataga tggtcatccg ctaactcctt aatccgggaa gttaatcaca
    49861 actttcccgc atttgccgct cgccatcagc gcataggcat cacctgcctg ttccagcgag
    49921 aagcgatggg taatggcgtt acgcggccac agcttccagt cagtcaaatc gtgggcgcat
    49981 ttttccatat ggaacagact ggtcacccag gagccaataa tccgccgttg atggtgcatc
    50041 agatcggcgc tgacctcgaa ttccactttt ccggtttcac caatgtaaac cacccgcccc
    50101 cagtcagcag tggattgcag tgccagcaag cgacctgcgg cattaccgga acaatcgagc
    50161 gcaacatccg cgccaccgtg ggtaagttca gcaattatct gcggcagacc ttcggtggtc
    50221 gctaaataac cgtgctccat cacccctaac tgttttgcca tcgccagacg ttccggcagc
    50281 atatcaacgc cgatgatccg ttttgcaccg cgacctttcg ccagcatcat cgccatcatg
    50341 ccgaccggcc ccagaccgac caccagcacg ttatcgctgc cggaaacttc gccgcgcaaa
    50401 atcccttcat aggccgtgcc aacgccgcaa ctgataaacg caccatcttc gtagctcagc
    50461 gcatccggca ggaggatcag atctttttct tccgccagca gatattcggc atgaccgccg
    50521 tcacgctgcc agccgtaagc cgcttttcct ttgccagtac aagagatagg aaagccgcgg
    50581 cggcagttcg ggcaaaaacc acagccagaa atgtgataca ccagcacgcg atcaccctct
    50641 ttaaaatggc gacagccttg ccccatcgcc acaatttgcc cgcacggctc atgaccgttg
    50701 ataaagccct ggtataacgg tttatcgggc gccgccgccg tggcgcggtg ttgatggtag
    50761 atatagtgga catcgcttcc gcaaatcccg gaggatttca ttttgatcag tacctggtta
    50821 atccccggcg tcggcaccgc aacttcccgc agatcgacgg tcgaatttcc tggtaaataa
    50881 gctgccagca tcgttttcat aaatccctca ttaacaatac gattaatttt catccctgcc
    50941 gcacccgcgc caggggcgtt aaggtttagc gtttcgcttt actgctacgc tgggtcagca
    51001 agatattcgc cagcaccgcc accacgatga tgacgccgcg caccacctgc tggaaaaagg
    51061 agttaatccc gagcagcacc agaccgttac cgattagcgt aatcaccagt acgccaagca
    51121 gcgtaccgaa caacgagccg cgaccaccgg aaagcgccgt accgccgacc acgaccgcgg
    51181 cgatgacgtc aaactccaga ccgtttgcgg cacctgcgtt accggaaccg aggcgcgccg
    51241 ccaacaaaat gccggtcacc gccgctaata atcccgaaag ggtaaagata agaatgcgta
    51301 cccgacgaac gttaataccg cacaactgcg ccgccgtggc attaccgcca acggcaaaaa
    51361 ccgagcgccc gaaggcggtt tttcggctaa tgaacacaaa cagcgcaaac agcaccatca
    51421 tgatcagcgc ggataccggc acaccgagaa attgtccgcc cagccagtcc agcacctcgt
    51481 tttcgtcaat cggcactggc agggcgttgg tcataaacaa gcccattccg cgcagggcgc
    51541 tccacagccc cagcgtggcc acgaaacttg gcacgttaaa cacgccacgc agcaccccgg
    51601 cgagcgttcc catcagcgcg cctaacagca acaccagcag acaagcaatc gccaacggaa
    51661 cttcaaattg cagcaaaaat gccaggcaca ccgagacaaa agccaccatc ggcccaacgc
    51721 tgacatcaat ttcaccggag ataataatca gcgtcatcgc ccaggcggca atcccaatgg
    51781 tggcggcatc gcgcagcacg ttcatctggt tattcaatga gataaagcca ggcgcgttca
    51841 gggagaagac cagataaaga atggcaatca ccaccagcaa accgatctca ttaatatggc
    51901 gactgacaaa ttgtttgagc gagacgctct tgccctgcgg caatggtaat gacgaagcag
    51961 acatcttagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    52021 atccacattg accggagcgt gaaactcctg cgagaacgta ccgtgctgta acaacagaat
    52081 gcggtcgcac accagcggta actcctccac ttcactggag ataaacacca cgctttttcc
    52141 ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    52201 gcgcgtcggc tcgtcgagca acaaaatccg gctggcggca tagacccaac gaccgatcac
    52261 cactttttgc tgattgccgc cagaaagcgt accgatgggc gtttcactac tggcggcctt
    52321 gaccgtcatc cgctgcatca cctcttcggt taggcggcgg atggtggacc attgcagcac
    52381 accgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca
    52441 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    52501 gtcgccgtaa tcggggtgcg tgattttctc gccgttgata acaatttcgc cctgttcata
    52561 ctcctccagc ccaacaatcg ctttcagcaa ttcactgcgt cctgccccca gcaggccagc
    52621 aatgccgagc acttcgccac gacgtagcgt aaagctgatg tcgtccagct tgggcttatg
    52681 gcgtaacgca cggatttcca gcacggcctg atccacaatt tcctgagggg caaccggcgc
    52741 aatatcaacg tgatcgcgcc cgagcatcag cgacacaata tgatgcgtgg aggtgttttc
    52801 gagcatcaca tcgcccgcca cctgaccatc gcgcatgacg gtggcacagg aggcaatgcg
    52861 gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt
    52921 tttcacggcg cttatcacca gttcaacttc cgcactggca agcgaactgg taggttcatc
    52981 aagaatgacc acgcgcggtt cgcctttcat cacccgcgca atttccacca gctgcttttg
    53041 cgccgggctt agccctgaaa caagttgttc agggctaacg tcaacgccca gcgcctgtaa
    53101 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca
    53161 ctgaccgagg cagaggtttt ccgccactgt cagcccttcc accaaactta actcctgata
    53221 aaccgcgcgt acccccagtt cagcggcacg gcgagtcaac gtagcttcgt cgccttccag
    53281 tcgcgtctca ccaatccaga tatcaccgct atccggacgt tcgctaccgg taagcattcg
    53341 aatgagggtc gatttgcccg cgccgttttt gcctaacagc gcacgaacct cgcctttatt
    53401 gagcgtgaag ttaacgttat ccaacgcaac gacgccggga taacgcttat ttcctgccac
    53461 catttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    53521 attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc gttttggtat
    53581 agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    53641 atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca
    53701 gcacctgatt gttttccagc tcctgagtaa tttcggttgt catatccgaa ccgaaaacag
    53761 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc
    53821 ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg
    53881 aaatcgcttt atctaaaaca gtcccttcct gattagcgac aaattgcgcg ccgggaacgc
    53941 gggcttttaa tacttcttca aaccctttac gtcgttgcac acaaacttca aaggcttcgc
    54001 aattgatgac ggcaattttc ggctggtcaa ttttgttggc aataaaataa tcggcggcag
    54061 cgttacccag ttttttacca aattccagcg ggtcacccac cagatatgcc gagacatatt
    54121 tatcgacccc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    54181 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat
    54241 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg
    54301 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct
    54361 gacgcactcc agcgtaatat ccctgagtat caaggtatat tgcgccaatg gtcatttctt
    54421 tttccgcagc cctggcaaat aaagcgctac ctaatagcgt agccattaat aatagattac
    54481 gggttgttct cattttttta ggcatagagc ctcctgtagg gtttttatta acaacggctt
    54541 attctaatta ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg
    54601 tttagtgaac gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc
    54661 aggccggatt aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga
    54721 gtcccgcgga cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
    54781 cgccatacag cgacaacaga tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg
    54841 ttttcgccat ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtgtgtt
    54901 gttcgcccag taatcgcagc gccattcctt gccagaagag gtaatcaacc ggctgatcgt
    54961 tgtaatagct gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacagcgcg
    55021 tcgcttcagt ttcatcaccc agggctttgg cacatatcgc ctgccagaac cagatgtcgt
    55081 tgtcagtttg ccccggtaat cggccttcgc ttaaattctc cggatagtgc agcgcggcat
    55141 gaagcagttt gctggcctgc tgcggttgtc tggcatcaag gtgttgccag gcgcgtaaca
    55201 actggttgag gataaactga ctggtgactt tcccttctcc gccttcccac gggtggaatt
    55261 tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgccc cgtgagatgc cacaaattga
    55321 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg
    55381 ccagtcgttt ctccggtgtg gctccactta acttatcaag caaatcccgt tcgaaaagca
    55441 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
    55501 aatcgtgttg cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg
    55561 gcgacatctc tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt
    55621 agtagaagca ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg
    55681 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg
    55741 cgaccagttc gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg
    55801 tgcgctggca gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt ccccagttaa
    55861 tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacacaga ccgcgccact
    55921 gcgcgagagc tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca
    55981 gcggataatc gcgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
    56041 acaccagcag cagattatgc aggcaaagca cttcctgatt ggttgggcag gcgcgaagac
    56101 tttgttggca aaaatccaga cccgcgtcga agttaccatt acgcgccgcc agtcgtgcca
    56161 gaccataata gcctccggct ttactgttgc cgctccagac cgcacgccag aaatcctctt
    56221 cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa ctggcctgtc
    56281 cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca
    56341 ccgcttgcgg gaaatctgcg cggttatatt ccagcatcgc cagcgccaga ttacagcggt
    56401 aatccagcga atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt
    56461 gatgatattg ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt
    56521 gtgctgacag tggcgctttg gcgacgtccg gcaacggcaa ctcttgcggc tgatgttcct
    56581 gataactcag tacaatattg ccgtcggcat cggagagctc aatggtcagc ctttcaggat
    56641 tgataccgtg caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcaa
    56701 gtaacgcgtt gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga
    56761 tggcatacag cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc
    56821 gggaggcatt ttgcaccatg cccagagaat gataaggcag gaaatactgc tcaaaacgct
    56881 tctcttcgta agcatcaagc caggtaaaat cgggctggtt atcggcaaaa ataccggtca
    56941 tcagttcgat atacgggccg ttattatcag ttaggctctt atcccacgcc tggccaaatt
    57001 cactgtgtcc ccagctccac tgctttttac ctggcgcaat atggtggttg gcaacgtgta
    57061 gcaaaccgcc atcttcatcg tgacaccacg cgccaacaaa atcgtactgc gatttttcgg
    57121 ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact ccagcggagt
    57181 agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt
    57241 gatcaaacac cgcagtcaca tccggcggga aaacgctctg atgcccttcc ccccctttca
    57301 ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc
    57361 tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
    57421 catgcatcgg ctccgtttcg cctacccaca ccgtctgtgc gccgtcttca tgggcttcga
    57481 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
    57541 tcccgccgga gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt
    57601 gatagacaaa atcgcgttgt ttcactttat cccatgcgcg atgcacccga ccgcccaatt
    57661 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca
    57721 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
    57781 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
    57841 tcggtatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
    57901 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
    57961 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
    58021 ccaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
    58081 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat
    58141 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa gccagacgcg
    58201 cgggcatagt agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgtctgaa
    58261 cgtgacgccc accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggtga
    58321 atttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
    58381 atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat
    58441 ccacggtcag gttgatccag tgacaggagt gtcgcaaacc atgccgcaag cgccgtggac
    58501 aacgccggtt gaggtgaagt atctgctgga agagaagctc ggcattcggg tgatggtcga
    58561 taatgactgc gtgatgctgg cgctggcgga aaaatggcaa aataattcgc aggaacggga
    58621 tttctgcgtg atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
    58681 ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg
    58741 cgtcgtctgc gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcttt
    58801 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga
    58861 aaaactgaca accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag
    58921 ctgggttgac cgcagtgcca atgccattgg tttgagtctg tataacttcc tcaacatcct
    58981 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa
    59041 tactattatt cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
    59101 agcgacgcaa attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta
    59161 tttgtatgtt gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc
    59221 cgaaggaagc ctttttcaac agattaaccg tttcgcttat gcgtaaaccg ggtaacgtgc
    59281 gcagatgtcg agaactttac ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc
    59341 cagcacgtca cacatccagc cagccagttc tttcgcttct gcttctttga agccgcgacg
    59401 cgtaatcgcc ggagtaccca cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt
    59461 cggtacgctg tttttgttga cggtgatgtt agcacggccc agagcggcgt ctgcttcttt
    59521 accggtcagg tttttatcga ccagatcaac caggaacaga tggttatcag taccgccgga
    59581 aaccactttg tagccgcgtt ccaggaacac ttctaccatc gctttagcgt ttttagcgac
    59641 ctgctgctgg taagttttga actcaggctc catcgcttct ttcagagcaa ccgctttacc
    59701 agcgattacg tgcatcaacg gaccgccctg accaccaggg aaaacggcag agttcagttt
    59761 tttgtacagc tcttcgctac cacctttcgc caggatcaga ccgccgcgcg gacccgccag
    59821 ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg ggtagacgcc
    59881 agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac aggtaagcac cgatgctgtc
    59941 agcgatttca cgcattttcg cccagtcaac cacgccggaa tatgcagaga aaccaccgat
    60001 aatcattttc ggcttgtgtt ctttggcttg tttttccaga tcggcgtagt cgatatgacc
    60061 ggtagcatcg ataccgtaag gaacgatgtt gtacagttta ccggagaagt taaccggaga
    60121 accgtgagtc aggtgaccgc catgcgccag gttcataccc agaacggtat cacctggttc
    60181 cagcagcgcg gtgtacaccg caaagttagc ctgggagccg gagtgcggct ggacgttagc
    60241 gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac
    60301 atactcgcaa ccgccgtagt agcgtttgcc cggataacct tcagcatatt tgttggtcag
    60361 ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc
    60421 gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt cggcatcata
    60481 atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct aacaataaaa
    60541 tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc gaatcattgt
    60601 gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttcaggc tacgcaaggc
    60661 tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg tgatgcaaat
    60721 ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat tgcagggcta
    60781 ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaagga agaccatatg
    60841 cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg
    60901 ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa
    60961 atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt
    61021 gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc
    61081 gcgcagaagc acaccagttt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac
    61141 ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt
    61201 aaagcctatg gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa
    61261 aacgccagca aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca
    61321 ccgcgcagcg cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca
    61381 gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag
    61441 gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg tattgcggtg
    61501 aaacgcgaag agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat
    61561 gtcgtgaaac tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca
    61621 gtgacgttaa tctctgccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg
    61681 gcaaaagcag gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgatgtt
    61741 cacgcctttg ccgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac
    61801 acctggtatc gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt
    61861 ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc
    61921 tgcggcccgg ttggcttcat gcagtttgcc gcgaaacagt tagtggatct gggcgtgaag
    61981 caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtgatt tgatgttgcc
    62041 ggatggaaac atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg
    62101 gtacggatgc gaatgacccg tgccacgtca aagacgaaga ttttaccgtc accgattttg
    62161 ccggtttgcg cagtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg
    62221 acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc
    62281 tcggtatggc ctttctggcg gccaaaacct ttcacctcag tgaccgtcat gccggtaatg
    62341 ccgacttcag ccagtgcttc gcggacatcg tccagcttga agggtttaat aatcgcatca
    62401 atctttttca tgctattcct tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac
    62461 ataacgccaa ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta
    62521 taaaattctg tccggttgcg ccccgccatt ctcgccgcat gggtgacgtt gcctttggta
    62581 atttgcagca gcttacgcaa atagttgagt tcaaactgat tacgtgcctc aacaaaggtt
    62641 ggcagcgccg tgttttcgcc ctccagcgcc tgctctacta atgcatcact aatcaccgga
    62701 gatgaggtca gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc
    62761 cagctcgcgg tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta
    62821 tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc
    62881 tctgccagag ccggaatttt caggctgaca acgttaaggc ggtaatagag atcttcacgg
    62941 aattccccgc gcaccatcgc ttttggcagg tcgcggtgag tggcggaaat aatccgcacg
    63001 tcgatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc
    63061 agcagtttga cctgcaacgg tgcgggcata tcgccaatct catcgagaaa tagcgtaccg
    63121 ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc
    63181 gcatgaccaa acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca
    63241 ataaatggtt tgctattgcg cgggctggcg ttgtggatag cctgggcgaa catctctttc
    63301 ccggtgccgc tctgaccgtt aatcaaaacg ctaacgtctg attgcgccac cagccgcgcc
    63361 tgttccagca aacgcagcat cagcgggctg cgggtgacaa tcgcctcgcg ccagcgttcg
    63421 tcggtggctg gcgcggattg ctccagcgca tcatcaattg cctgatatag cgcgtcttta
    63481 tcgacaggct tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcggga
    63541 atagaaccat gcgcggtaag aataattacc ggcattcccg gctgcacttt ctggatttca
    63601 gcaaacagct gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact
    63661 ttttcgcgat tcagtacccg taaaccttca gcgccacttt ccgccgtgac cacactgtag
    63721 ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat
    63781 aataaatgcg caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt
    63841 ttcactctcg tgtggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg
    63901 gcgttcaata tcagtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag
    63961 aacgtgatgt tgctggcgca gagcgtccag ctcgctgtcg ctggactgct ggagtttact
    64021 gtagcgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg
    64081 atacagtggg cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag
    64141 ctggcggcgc tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt
    64201 attttgccag ctgccgtcgt catattggcg ggcttgctga cgcgactgtg caggcattaa
    64261 acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcag ttgatttacc
    64321 ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg
    64381 aattttttct tctgctggcg tatcaatggc tggcttattg tgactctgca cgcaacccag
    64441 caatgccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat
    64501 gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc
    64561 tggacatgga cagcgcggag aaaccggagc gtacatgtcg tacgtgagga tttcgagcac
    64621 tgcccggggc cgaactgaca aataaaatag cctgatggga tagactgtta gcattatttc
    64681 gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc
    64741 agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg
    64801 ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg
    64861 gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgcg
    64921 ccatgtaaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg
    64981 gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct
    65041 ttgagatcga cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc
    65101 actgtctcca ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt
    65161 ttacggttgt aatcaagcag ttgttcgatc agtttttgca aattgcggct gctgctatca
    65221 agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt
    65281 aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt
    65341 aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt
    65401 tgcccaaccg agcgtaactc gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc
    65461 ccttccccca accgattgat catccgttca atatttttca ccggcccgat aatcatccgc
    65521 gtgaaaagta gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa
    65581 tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg
    65641 cgcgtggcct gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg
    65701 gcagcatcgg gaccactgtt gttgcactga agttgggcca gattgttcaa gtcctgacgc
    65761 aatgcctggt aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg
    65821 taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcatccag cacgcaatac
    65881 tgacggtagc tacgctccat ctccagcgcc gcgttggtca ttgcttcgct gcgccgggca
    65941 tcgataagcg tggtgcggtt gaccagcgcc gcctggtcgc taagcgcatt caggctttgc
    66001 caggcttgcc atgccagcac caacaggggc agcagaatca gcaaaaatgc cagcattacc
    66061 agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac
    66121 gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg
    66181 gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct
    66241 acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta
    66301 gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgattact attatgcagt
    66361 aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgaggggt
    66421 ttatggcttc cccgctattt gaatgtaaat cagccactgt gtctctaaaa agagacaact
    66481 taagaaaaac ttattagatt acaataaaat caatcattta agtgtcgcca gaaagcgaca
    66541 cggtaaacca cccattgtcg tgatttacag acacaaaaaa gctcccgaag ttgggagctt
    66601 ataatagcgg ttgtatttaa tgccggatgc ggcgtgaatg cctcatccgg gctgcaaaac
    66661 caatgagttt gacggcttac cccaactgct tacgtgcatt gcggaaaatg cgcatccatg
    66721 ggccatcctc gccccagttt tcaggatgcc aggagttgct gacagtacgg aaaacacgtt
    66781 ccgggtgcgg catcataatg gtgactcgac cgctttcagt cgtgaccgcc gtaataccgt
    66841 tcggagaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag ttatcgacat
    66901 agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca tcacgcactt
    66961 ccacgcgccc ttcaccatga gagacagcaa tcggcatttg cgagcccacc atcccctgca
    67021 acagcagcga cgggctttgg gtcacttcaa ccaggctgaa acgcgcttca aagcgatcgg
    67081 aggtattgcg cacaaaacgt ggccacaact cactacctgg gatcagttca cgcagattag
    67141 acatcatctg gcaaccgtta cataccccca gcgccagcgt ttgcggacgg tggaagaagg
    67201 ttgcaaactc atcgcgtaca cggtcattga acaggataga cttcgcccaa ccttcaccgg
    67261 cacccagcac atcaccgtag gagaaaccac cgcacgcgac cagggcgtgg aaatcctcca
    67321 ggcccgtgcg tccagccagc aggtcgctca tatgcacgtc gatagcatcg aagcctgcac
    67381 ggtggaaagc tgccgccatt tcaacatgcg agttcacgcc ctgctcacgc agcacagcaa
    67441 ctttcggacg tgcgccagtg gcaatatacg gtgctgccac atcttcgttg atatcgaacg
    67501 acagttttac attcaggccc ggatcggcgt cgtttgattt cgcctgatgc tcctgatcgg
    67561 cacactccgg gttgtcacgc aggcgctgca tctgccaggt agtttctgcc caccagacac
    67621 gcaacgtagt gcggctttcg ctgaatacag tctgcccgtt ggcggtaatc acaaaacggt
    67681 caccggaaac cgcctgccca acataatgga cgcaatcagc aagcccatgc tgtgccagta
    67741 cggactcgac cgcttcacgg tcagcggcac gaacctgaat gaccgcaccc agttcttcgt
    67801 taaacaacgc cgccaggcga tcgtcaccca gagtggcgat atccgcgtaa atgccgcaat
    67861 gaccagcaaa ggccatttcc gccagcgtta ccagcagacc gccatcagag cggtcgtgat
    67921 acgccagcag cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag cctttcagtt
    67981 gcgcgacatc gcgtacatct gccggtttgt cgccaagctg acgataaacc tgcgccagcg
    68041 ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcagcagt gcgttatctt
    68101 cggtagaaag ctgcggcgtg atggtgtgac gtacatcttc cacgcgggca aatgcagaaa
    68161 tcaccagcga cagcggcgac gtcatttcgc gctcttcgtt accttcctgc cagcgggttt
    68221 tcatcgacat agagtcttta cccaccggga tcgtcaggcc cagcgccgga caaagctctt
    68281 cgcccacggc tttaacggct tcatacaggc ccgcatcttc accagggtgg cctgccgccg
    68341 ccatccagtt ggcggaaagt ttaatgcgtt tgatatcgcc aatctgtgtt gcggcaatat
    68401 tggttaacgc ttcaccgacc gccagacggg cagaggcggc gaaatccagc agcgcaaccg
    68461 gcgcacgctc gccaatcgcc atcgcttcac cgtagtagct gtcgaggctg gcggtagtga
    68521 ccgcgcagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc gccaccatgc
    68581 cggttacgct gcggtcaccg atggtcacca ggaaagtttt ctccgccacg gtcggcagat
    68641 gcagtacgcg tttcaccgcg tctgcaatgg tgatcccttc acggaccagc gcatcgccct
    68701 tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt tttaccaagc aggacgtcca
    68761 gcggcagatc gatcggctga ttatcaaaat gacgatcgtg cagagaaaga tgcagttctt
    68821 cggtcgcttc accaatcacc gcgtagggtg cgcgctcacg cttacacagt tcgtcaaaca
    68881 gcggtaactg atcggcagca accgccagca cgtagcgttc ctgggattcg ttacaccaga
    68941 tttccagcgg gctcatgccc ggttcgtcgc tcaggatatc gcgcagttca aatttaccgc
    69001 cgcgcccgcc gtcgcttacc agttccggca tcgcgttaga aagaccgcct gcaccaacgt
    69061 cgtggataaa caggattggg ttggcatcac caagctgcca gcaacgatcg atcacttcct
    69121 ggcagcgacg ctccatttcc gggttgtcgc gctggacgga agcaaagtcg aggtcggcat
    69181 cagactgacc tgacgccatg gaagacgccg ctccgccgcc aagaccgatg ttcattgccg
    69241 ggccgccgag aacgaccagc ttcgcaccga cgttgatctc gcctttttgt acgtgatcgg
    69301 cgcgaatgtt gccgatcccg cccgccagca tgatcggttt gtgataaccg cgcagctctt
    69361 cgccgttgtg gctgttcact ttttcttcat aagtacggaa gtagccattc agtgccggac
    69421 gaccaaattc gttgttaaac gccgcgccgc ccagcgggcc ttcggtcatg atgtccagcg
    69481 cggtgacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttcg aagcccggaa
    69541 tacgcaggtt ggaaacggag aaaccgacca gaccagcttt cggcttcgcg ccacgcccgg
    69601 tggcgccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc cacggtgaaa
    69661 tcgccgtcgg gtggttgtgg gtttcaactt tcatcaggat atgcaccggt tcctgatgga
    69721 aatcgtagcg gcccgtttcg tggtcggcaa aataacggcc cacttcagaa ccttccatta
    69781 cggcggcgtt atctttataa gcagagagaa cgtgatctgg cgtggtttcg aaagtatttt
    69841 tgatcatcct gaacagcgat ttcggctgct gttcaccgtc gataatccag tcggcattaa
    69901 aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa catatacagc tcgatgtcgt
    69961 tcgggttacg accaagcttt gtgaaagcat cctgcagata gtcaatttca tcttccgcca
    70021 gagccaggcc aagacgcagg ttagcgtcga tcagcgcctg acggccctgc cccagcaaat
    70081 caacgctggt aaccggagtc ggttgatggt gggcaaacaa ctgctctgca tcatctaaag
    70141 caaaaaagac cgtttccatc atgcggtcgt gcagttcagc ggtaacctgc tgccattgtt
    70201 cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg cggtttacct
    70261 gttgtagccc gcagttatgg gcaatatcgg tcgctttcga agaccaggga gagatggtgc
    70321 caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg agtgccgggc
    70381 catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc gcattgaggt
    70441 cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca gcctgaaaac
    70501 gtgccagcag tttgttgatt cggaatgccg acagtgcagg cgaaccacgc agaatttcca
    70561 tcataagtct ctcgtcttct aagctttcgg tgtacccaag ggggggaaac gggcgtcatt
    70621 ataaagaatc tgatgcgctg acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat
    70681 tagcaacgcg tgccgccaat ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa
    70741 tgccgctcat ttatgagtaa acctttcact attattacgt tttttcaagc tgggacgcgc
    70801 acgacacaga gaattaacta attgaaaaaa ttaaagatta attatctgtt catcggcatt
    70861 ctggcactgc tgctcgcggt cgctctctgg ccatccattc cctggtttgg taaagccgac
    70921 aaccgtatcg ccgccattca agcgcgggga gagttgcgtg tgagcaccat tcatactccc
    70981 ctgacttata acgaaatcaa cgggaaacct tttggcctgg attacgaact ggcgaaacag
    71041 tttgccgatt acctcggtgt aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg
    71101 tttgacgacc tcgataatgg taacgccgac ctgctggcgg caggacttgt ctataacagt
    71161 gagcgggtaa aaaattatca gcctggccct acctattatt ccgtgtcaca acaactggtt
    71221 tataaagtgg gtcagtatcg cccacgtacg ctgggcaacc tgacggcgga gcaactcacc
    71281 gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc tgaaagaaac aaaattcccg
    71341 gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc
    71401 gaaggaaaac tcgattacac cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt
    71461 cacccggagc tcgccgtagc gctcgatatc accgatgaac aaccggtgac ttggtttagc
    71521 ccgttagatg gcgataatac cctttccgcc gccctgctcg acttcttcaa cgaaatgaat
    71581 gaagacggta cgctggcacg cattgaagag aaatacctgg ggcatggcga tgattttgat
    71641 tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc
    71701 ctgtttgaga aatacgccga agaaattgac tggcgtttgc tggccgctat tgcttatcag
    71761 gaatcgcact gggatgcaca ggccacttca ccgacgggtg tgcgcggcat gatgatgtta
    71821 accaaaaata ccgcgcaaag cctcggcatt acggatcgta ccgatgccga acagagcatc
    71881 agcggtggcg tgcgttattt gcagcatatg atgagtaaag tgccggaaag tgtgccggag
    71941 aacgagcgga tctggtttgc cctcgctgcg tacaatatgg gctatgcgca tatgctggat
    72001 gcccgcgccc tgacggcaaa aaccaaaggg aatcctgaca gttgggctga cgtaaaacag
    72061 cgtctgcctt tacttagcca gaaaccctat tacagcaagc tgacttacgg ctacgctcgt
    72121 gggcatgaag cctacgctta tgtcgaaaat attcgtaagt atcagattag cctggtgggt
    72181 tatctgcaag agaaagagaa gcaggctaca gaagcggcga tgcaactggc gcaggattat
    72241 ccggcggtat cgcctacgga gttgggcaaa gagaaatttc cttttctctc gtttctttcc
    72301 cagtcgtcat caaactattt gacccattct ccctctctgc tgttttccag gaaagggagt
    72361 gaagagaaac aaaattaatc cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg
    72421 cggcgcatgc gaaagaagtc actgagcaac gccgcgcact catccgccag tattccttcc
    72481 gtaatttcca ctcggtgatt catacccgga tgatgcagca catccattaa agatcccgca
    72541 gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac caatgcgact gtggatcatc
    72601 gctccggcac acattacaca tggttcaagc gtgacataca acgtggcgtc gatcagacga
    72661 taattttgca tcaccagacc gccctgcctc agggccatga tttctgcatg tgcggtggga
    72721 tcatgtcgac caatcggacg gttccagcct tcgccaataa cacgattgtt atgcactaat
    72781 accgcgccga ccggtacttc ccgctcatcc caggcacgct tcgccagcgt cattgcatga
    72841 cgcatccagt attcgtggct aaattcgact tcagacaaaa agaaaactcc ggttataaaa
    72901 gcgcggcgca ttatacacgg acgctatgct ttactctatt ccagttgctg gagttcaccg
    72961 cgcggggtta ctcgccagcg atgctggcaa aagtaaagca gcgggttgtc ctgattactg
    73021 tcgctatagc cactgtacag ccgcagcggc gtgccgattt tgcgctccag ttgcgcgacc
    73081 ttttcatgtc ccagacaacg catcgtcaat acccaaccac catagccacg ctgaatttgg
    73141 ctggcgataa gattaactcg cggcagccag ggcgtatcga aataaaccgc ttcaaccagc
    73201 ggctgcggag agccggtaat caaccagata tcagcatcgg aacttaacag gtaggtggtt
    73261 aatcgctcct gaaccagcgg aaaggcggta acattgtcac gaaaccagcg tacgaaatcg
    73321 gcctgcaacg cctgcaaacg cgcttcattg tgaccaaaag tgcatcccca cagaagcaga
    73381 ctcatcggcc agcgtgccgc acgacctttt atcaataacg caatggctat aatcggcaac
    73441 aacggcagga caagtaacgc attcagcggt tggcgacgca gtaaatagcg cagaaaactg
    73501 ccgaacatat cctgctgatg caatgttcca tctaaatcaa aaaacaccac acgacgctcg
    73561 tgagttgcca aaccatactc ctctggactg aaacatcctg attaattttc gctactacat
    73621 agcctaacag atagatcatc acttttccgg caacaatcga ccgcatcatg gctaactgga
    73681 atttttaatt catggcaatt agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat
    73741 attgcctgtt gtcgccacta tttcatcgcg aggaaagata tgaacggctt acttcgtatc
    73801 cgtcagcgtt accaggggct tgcccagagt gataaaaaac tggcggatta tctgctgcta
    73861 caacctgata cggcgcgcca tttaagctct cagcaactgg ccaacgaagc cggagtcagt
    73921 cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag
    73981 ttggcgttga gtgaagcgct ggcaagccag ccggaatcgc cctcagtgcc cattcataac
    74041 caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa aactgattaa agaaaatact
    74101 gccgctatgt acgcaacgct aaacgttaat agtgaagaga aactgcatga atgcgtaaca
    74161 atgttgcgct ctgcgcggcg gataattctg accggtattg gcgcttcggg tctggtggcg
    74221 caaaactttg cctggaagct gatgaagatt ggcttcaatg ctgccgcagt gcgcgatatg
    74281 catgcgctac tcgctaccgt acaggcgtcg tcccctgacg atctgttatt agccatttct
    74341 tacaccggtg tacgacgcga gttaaacctg gcggcagatg agatgctgcg agtgggcgga
    74401 aaagtgctgg cgattaccgg ctttactccc aatgccctgc aacagcgtgc ttctcattgc
    74461 ctgtatacca ttgccgaaga acaagcgaca aacagtgctt caatctctgc ttgtcacgcg
    74521 cagggaatgt taacggattt gctgttcatt gcgctgattc agcaggatct ggaactggca
    74581 ccagaacgta ttcgtcatag tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg
    74641 cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc cttatggcgc tgttaatcac
    74701 taaaaaatgc atcaattgtg atatgtgtga aatcatataa aaacacattt attttcatag
    74761 tgatacagta atgtgttatt gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat
    74821 aagttcagca acacaaagcc tccccctttc tttagcattt tctactgagc agccatccgc
    74881 tttcagacct cacttaaatt acgctta
//
LOCUS       DAZGCQ010000009        72987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000009 DAZGCQ010000000
VERSION     DAZGCQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.009"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..735
                     /gene="lpfA1"
                     /locus_tag="ACHK07_000658"
     CDS             211..735
                     /gene="lpfA1"
                     /locus_tag="ACHK07_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO8101036.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            800..1486
                     /gene="lpfB"
                     /locus_tag="ACHK07_000659"
     CDS             800..1486
                     /gene="lpfB"
                     /locus_tag="ACHK07_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO8101037.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RVDNPTVFNITFNQFFANEKEIEKAGMVPAKGSLNITLPAGAGSVSKIKYNAINDFGS
                     SAEMMTKNVD"
     gene            1515..4088
                     /gene="lpfC"
                     /locus_tag="ACHK07_000660"
     CDS             1515..4088
                     /gene="lpfC"
                     /locus_tag="ACHK07_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO8101038.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYVDDNGTVHHDDGNDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIEKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIIPVSANC
                     Q"
     gene            4103..5158
                     /locus_tag="ACHK07_000661"
     CDS             4103..5158
                     /locus_tag="ACHK07_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101039.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRSV
                     DSMKYYTLTEELEVATKIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5163..5693
                     /gene="lpfE"
                     /locus_tag="ACHK07_000662"
     CDS             5163..5693
                     /gene="lpfE"
                     /locus_tag="ACHK07_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO8101040.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5948..7639
                     /gene="eptB"
                     /locus_tag="ACHK07_000663"
     CDS             5948..7639
                     /gene="eptB"
                     /locus_tag="ACHK07_000663"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO8101041.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            7731..7807
                     /locus_tag="ACHK07_000664"
     tRNA            7731..7807
                     /locus_tag="ACHK07_000664"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7765..7767,aa:Pro,seq:cgg)
     gene            8552..10159
                     /gene="dppA"
                     /locus_tag="ACHK07_000665"
     CDS             8552..10159
                     /gene="dppA"
                     /locus_tag="ACHK07_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO8101042.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10467..11486
                     /gene="dppB"
                     /locus_tag="ACHK07_000666"
     CDS             10467..11486
                     /gene="dppB"
                     /locus_tag="ACHK07_000666"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO8101043.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11496..12398
                     /gene="dppC"
                     /locus_tag="ACHK07_000667"
     CDS             11496..12398
                     /gene="dppC"
                     /locus_tag="ACHK07_000667"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO8101044.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12409..13392
                     /gene="dppD"
                     /locus_tag="ACHK07_000668"
     CDS             12409..13392
                     /gene="dppD"
                     /locus_tag="ACHK07_000668"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO8101045.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13389..14393
                     /gene="dppF"
                     /locus_tag="ACHK07_000669"
     CDS             13389..14393
                     /gene="dppF"
                     /locus_tag="ACHK07_000669"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO8101046.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14423..15694)
                     /gene="yhjV"
                     /locus_tag="ACHK07_000670"
     CDS             complement(14423..15694)
                     /gene="yhjV"
                     /locus_tag="ACHK07_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO8101047.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16170..16277
                     /locus_tag="ACHK07_000671"
     CDS             16170..16277
                     /locus_tag="ACHK07_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8101048.1"
                     /translation="MTLAELGMAFWHDLVAPVIAGILASMIVNWLNKRK"
     gene            16653..16760
                     /locus_tag="ACHK07_000672"
     CDS             16653..16760
                     /locus_tag="ACHK07_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8101049.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17136..17243
                     /locus_tag="ACHK07_000673"
     CDS             17136..17243
                     /locus_tag="ACHK07_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8101050.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17330..19009)
                     /gene="bcsG"
                     /locus_tag="ACHK07_000674"
     CDS             complement(17330..19009)
                     /gene="bcsG"
                     /locus_tag="ACHK07_000674"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO8101051.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19006..19197)
                     /gene="bcsF"
                     /locus_tag="ACHK07_000675"
     CDS             complement(19006..19197)
                     /gene="bcsF"
                     /locus_tag="ACHK07_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO8101052.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19194..20765)
                     /gene="bcsE"
                     /locus_tag="ACHK07_000676"
     CDS             complement(19194..20765)
                     /gene="bcsE"
                     /locus_tag="ACHK07_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO8101053.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21038..21226
                     /gene="bcsR"
                     /locus_tag="ACHK07_000677"
     CDS             21038..21226
                     /gene="bcsR"
                     /locus_tag="ACHK07_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO8101054.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21238..21990
                     /gene="bcsQ"
                     /locus_tag="ACHK07_000678"
     CDS             21238..21990
                     /gene="bcsQ"
                     /locus_tag="ACHK07_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO8101055.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKSSGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            21987..24605
                     /gene="bcsA"
                     /locus_tag="ACHK07_000679"
     CDS             21987..24605
                     /gene="bcsA"
                     /locus_tag="ACHK07_000679"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO8101056.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24616..26955
                     /gene="bcsB"
                     /locus_tag="ACHK07_000680"
     CDS             24616..26955
                     /gene="bcsB"
                     /locus_tag="ACHK07_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO8101057.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            26962..28068
                     /gene="bcsZ"
                     /locus_tag="ACHK07_000681"
     CDS             26962..28068
                     /gene="bcsZ"
                     /locus_tag="ACHK07_000681"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO8101058.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            28050..31523
                     /gene="bcsC"
                     /locus_tag="ACHK07_000682"
     CDS             28050..31523
                     /gene="bcsC"
                     /locus_tag="ACHK07_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO8101059.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLHQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31605..33593
                     /gene="hmsP"
                     /locus_tag="ACHK07_000683"
     CDS             31605..33593
                     /gene="hmsP"
                     /locus_tag="ACHK07_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO8101060.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            33776..35062
                     /gene="dctA"
                     /locus_tag="ACHK07_000684"
     CDS             33776..35062
                     /gene="dctA"
                     /locus_tag="ACHK07_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO8101061.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35283..36779
                     /gene="yhjJ"
                     /locus_tag="ACHK07_000685"
     CDS             35283..36779
                     /gene="yhjJ"
                     /locus_tag="ACHK07_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8101062.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(36875..37804)
                     /gene="kdgK"
                     /locus_tag="ACHK07_000686"
     CDS             complement(36875..37804)
                     /gene="kdgK"
                     /locus_tag="ACHK07_000686"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO8101063.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            38036..38803
                     /gene="pdeH"
                     /locus_tag="ACHK07_000687"
     CDS             38036..38803
                     /gene="pdeH"
                     /locus_tag="ACHK07_000687"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO8101064.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            38873..40933
                     /gene="yhjG"
                     /locus_tag="ACHK07_000688"
     CDS             38873..40933
                     /gene="yhjG"
                     /locus_tag="ACHK07_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8101065.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(41115..42437)
                     /gene="yhjE"
                     /locus_tag="ACHK07_000689"
     CDS             complement(41115..42437)
                     /gene="yhjE"
                     /locus_tag="ACHK07_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101066.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(42830..43843)
                     /gene="yhjD"
                     /locus_tag="ACHK07_000690"
     CDS             complement(42830..43843)
                     /gene="yhjD"
                     /locus_tag="ACHK07_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO8101067.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(43892..44791)
                     /gene="rcdB"
                     /locus_tag="ACHK07_000691"
     CDS             complement(43892..44791)
                     /gene="rcdB"
                     /locus_tag="ACHK07_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8101068.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            45311..45913
                     /gene="yhjB"
                     /locus_tag="ACHK07_000692"
     CDS             45311..45913
                     /gene="yhjB"
                     /locus_tag="ACHK07_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO8101069.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(45964..47613)
                     /gene="treF"
                     /locus_tag="ACHK07_000693"
     CDS             complement(45964..47613)
                     /gene="treF"
                     /locus_tag="ACHK07_000693"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8101070.1"
                     /translation="MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            48018..49415
                     /gene="ccp"
                     /locus_tag="ACHK07_000694"
     CDS             48018..49415
                     /gene="ccp"
                     /locus_tag="ACHK07_000694"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO8101071.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            49626..51026
                     /gene="gadA"
                     /locus_tag="ACHK07_000695"
     CDS             49626..51026
                     /gene="gadA"
                     /locus_tag="ACHK07_000695"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO8101072.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            51396..52220
                     /gene="gadX"
                     /locus_tag="ACHK07_000696"
     CDS             51396..52220
                     /gene="gadX"
                     /locus_tag="ACHK07_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO8101073.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLPNRDSAASIVAQGNFYGADCSAEGIRL"
     gene            52588..53316
                     /gene="gadW"
                     /locus_tag="ACHK07_000697"
     CDS             52588..53316
                     /gene="gadW"
                     /locus_tag="ACHK07_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO8101074.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(53679..56792)
                     /gene="mdtF"
                     /locus_tag="ACHK07_000698"
     CDS             complement(53679..56792)
                     /gene="mdtF"
                     /locus_tag="ACHK07_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO8101075.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGADDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(56817..57926)
                     /gene="mdtE"
                     /locus_tag="ACHK07_000699"
     CDS             complement(56817..57926)
                     /gene="mdtE"
                     /locus_tag="ACHK07_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO8101076.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            58034..58312
                     /locus_tag="ACHK07_000700"
     CDS             58034..58312
                     /locus_tag="ACHK07_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101077.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(58313..58840)
                     /gene="gadE"
                     /locus_tag="ACHK07_000701"
     CDS             complement(58313..58840)
                     /gene="gadE"
                     /locus_tag="ACHK07_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO8101078.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(59639..60211)
                     /gene="hdeD"
                     /locus_tag="ACHK07_000702"
     CDS             complement(59639..60211)
                     /gene="hdeD"
                     /locus_tag="ACHK07_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO8101079.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            60466..60798
                     /gene="hdeA"
                     /locus_tag="ACHK07_000703"
     CDS             60466..60798
                     /gene="hdeA"
                     /locus_tag="ACHK07_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO8101080.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            60914..61240
                     /gene="hdeB"
                     /locus_tag="ACHK07_000704"
     CDS             60914..61240
                     /gene="hdeB"
                     /locus_tag="ACHK07_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO8101081.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            61304..61951
                     /gene="yhiD"
                     /locus_tag="ACHK07_000705"
     CDS             61304..61951
                     /gene="yhiD"
                     /locus_tag="ACHK07_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO8101082.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQIRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(62003..62773)
                     /locus_tag="ACHK07_000706"
     CDS             complement(62003..62773)
                     /locus_tag="ACHK07_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO8101083.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(62770..63762)
                     /gene="shuU"
                     /locus_tag="ACHK07_000707"
                     /gene_synonym="chuU"
     CDS             complement(62770..63762)
                     /gene="shuU"
                     /locus_tag="ACHK07_000707"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO8101084.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(63811..64434)
                     /gene="chuY"
                     /locus_tag="ACHK07_000708"
     CDS             complement(63811..64434)
                     /gene="chuY"
                     /locus_tag="ACHK07_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO8101085.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(64434..64928)
                     /gene="hutX"
                     /locus_tag="ACHK07_000709"
     CDS             complement(64434..64928)
                     /gene="hutX"
                     /locus_tag="ACHK07_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO8101086.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPFSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(64941..66278)
                     /gene="hutW"
                     /locus_tag="ACHK07_000710"
     CDS             complement(64941..66278)
                     /gene="hutW"
                     /locus_tag="ACHK07_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO8101087.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(66298..67212)
                     /gene="shuT"
                     /locus_tag="ACHK07_000711"
     CDS             complement(66298..67212)
                     /gene="shuT"
                     /locus_tag="ACHK07_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO8101088.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRITDVVESLHQQLWPQ"
     gene            67894..69876
                     /gene="chuA"
                     /locus_tag="ACHK07_000712"
                     /gene_synonym="shuA"
     CDS             67894..69876
                     /gene="chuA"
                     /locus_tag="ACHK07_000712"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO8101089.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            69925..70953
                     /gene="chuS"
                     /locus_tag="ACHK07_000713"
     CDS             69925..70953
                     /gene="chuS"
                     /locus_tag="ACHK07_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO8101090.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAV"
     gene            complement(71014..71544)
                     /gene="dctR"
                     /locus_tag="ACHK07_000714"
     CDS             complement(71014..71544)
                     /gene="dctR"
                     /locus_tag="ACHK07_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8101091.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(71700..72266)
                     /gene="slp"
                     /locus_tag="ACHK07_000715"
     CDS             complement(71700..72266)
                     /gene="slp"
                     /locus_tag="ACHK07_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO8101092.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(72620..72987)
                     /locus_tag="ACHK07_000716"
                     /pseudo
     CDS             complement(72620..72987)
                     /locus_tag="ACHK07_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        18207 a        17734 c        19022 g        18024 t
ORIGIN      
        1 ttgttttttc ctaaaaatca ttcctgattt tttatcccga aaggcaactt agtgatttaa
       61 atcaaaattc acactttctt aatttattga tattttatcc tcttaatttt cttttccttg
      121 tttatttgta taatcccgcc ttcaaagttg atgcaatgtt aaagcatcac tatttgctaa
      181 ttaatgtgat ttaataaaat ggagtttttc atgaaaaagg tcgtttttgc cttaactgca
      241 ctggcgttaa cctccggctc tgcatttgca gcagaatctg gtgacggcac ggttaaattt
      301 acaggcgaga tcgtggattc accttgcgta ctgtccgttg actctcagaa ccaggaagtt
      361 gtgttgggtc aggtacaaaa atctgtattt gctgcggttg gtgacaaatc cccggcaaaa
      421 cctttcgaaa tcaaactgga agattgcgat accaccacga tgaaaaaagc caatgtgagc
      481 tttagcggtg ttggtgatgc agatcaatct gatctgattt ctgttagtac tgaagccggt
      541 gcggcgaaag gtgttggtat cggtatttat gataacagca acaccctagt cgctcttaac
      601 ggtggtaaag caagtgttga tctgagtaaa ggccagacgg ttctctactt taccgccaac
      661 tacgtttcaa ctctggcaac ggtaacgacc ggttacggca atgcgcaggt cgatttcaac
      721 ctgtcttacg agtaatcgtc tgttttaccc atggcggcgt aattgccgcc attagtcgtc
      781 aaaattcatg gatcgaatga tgaaaagtaa atttgttgcg ttggctctgt ctctgttcct
      841 ctcccagtca gttctggcgg ggggcgttgg gcttagtgct acccgtgtga tttatgatgg
      901 cagtaagaaa gaagcctcgc tgaccgtaca gaataaaagt aagacggaag agtttctgat
      961 ccagtcctgg gtggatgatg ccgcgggcag caaaaaaaca ccgtttataa ttaccccgcc
     1021 gttgttcagg ctcgatcctg gaaaaaacaa tatattgcgt attgtaaata ctacccctgg
     1081 cctgccgcag gatcgcgaat cggtctactg ggttaatgtg aaagccattc cgtctaaaag
     1141 tgacgacagc gaaaacaaaa atgtattaca gattgctgtt cgcacccgca tcaaactgtt
     1201 ttatcgcccg gcagggttac agggtgatgt aaaaaccgcg ccaaacgaac tgcgatttac
     1261 gcgtagcggt aatcagatcc gtgtcgataa ccccaccgtt tttaatatta cctttaatca
     1321 attttttgcg aatgaaaaag aaattgaaaa agccggcatg gtacccgcaa aagggtcatt
     1381 aaatattaca ttacccgccg gggcaggtag cgtttcgaag attaaatata acgccatcaa
     1441 tgactttgga tcgtcagcgg aaatgatgac taaaaatgtt gattgattaa taccttttca
     1501 ggagaaattg ctcaatgtca cggaagaccg tttcacgtac tttttcttcc ttttctatct
     1561 ctgtggtggc ggttgctgtt gccagcacgt tttctgcaca tgccggaaag tttaacccca
     1621 aattcctgga ggacgttcag ggtgtgggcc agcatgtcga tttaacgatg ttcgaaaaag
     1681 gccaggaaca gcagttaccc ggcatctatc gtgtctctgt ttacgtcaat gaacagagaa
     1741 tggaaacccg cacactggaa tttaaagagg ccacagaagc acagcgcaaa gcgatggggg
     1801 aatcccttgt gccttgcctt agccggacgc aactggcaga gatgggcgtg cgcgttgaaa
     1861 gctttccggc cctcaatctg gtgcctgccg aagcctgcgt gccgtttgac gaaatcattc
     1921 cccaggcctc aagccatttc gacttcagtg aacaaaagct ggtattaagt ttccctcagg
     1981 cggcgatgca ccaggtggcg cgcggcacgg tgccggaatc tttatgggac gaaggtatcc
     2041 cggcattatt gctggattac agttttagcg ggagcaacag tgaatatgac tcctcgggtt
     2101 cttccagcag ttatgtcgat gataatggca ccgttcatca tgatgatggc aacgatacgc
     2161 taaagagcga cagctactac ctcaacttac gcagcggcct gaaccttggc gcgtggcggt
     2221 tacgtaatta cagtacctgg agccattccg gtggcaaagc gcagtgggat aatatcggta
     2281 cgtctttgag tagggccatc atccctttca aagcgcaatt aacgatgggg gatacggcga
     2341 ccgcggggga tatttttgac agcgttcaga tgcgtggcgc gatgttagcc tctgatgaag
     2401 agatgctgcc cgacagccag cgaggctttg cacccatcgt tcgagggatc gccaaaagca
     2461 acgccgaagt cagtattgaa caaaacggct acgttattta tcgcacctat gtgcagcctg
     2521 gtgcctttga aataaacgat ctttacccca ctgcaaacag cggtgatctc acggtcatta
     2581 ttaaagaagc ggatggcagt gaacaacgct ttattcagcc attctcatcg gtgccaattt
     2641 tccagcgtga aggccatctc aaatatagct ttgccgcagg tgaatatcag gccgggaatt
     2701 atgacagcgc ctcgccgcgt ttcgggcagc ttgatctgat ctacggttta ccgtggggga
     2761 tgacggccta cggcggcgta ttaatctcta ataattacaa tgcatttgca ttagggatag
     2821 agaaaaactt tggttatatc ggggcgattt ccattgatgt gacgcaggct aaaagcgaac
     2881 tgaataacga tcgcgatagc cagggacaat cttatcgttt cttatattcc aagagcttcg
     2941 aaagcggcac cgatttccgc cttgcgggct atcggtactc taccagcggt ttctatacct
     3001 tccaggaagc caccgatgtg cgcagtgacg ctgacagcga ctataaccgt tatcacaagc
     3061 gcagcgaaat acagggtaac ctgacgcagc aattaggggc ctatggctct gtttatttaa
     3121 atttaacgca gcaggattac tggaacgacg caggtaaaca gaacacggta tcggcgggtt
     3181 acaacggacg tattggcaag gtcagttaca gtattgcata tagctggaat aaaagccctg
     3241 aatgggatga aagcgatcgc ttgtggtctt tcaatatttc cgttccacta ggccgggcct
     3301 ggagtaacta tcgcgtcacg accgaccagg atggtcgtac caatcaacag gttggggtca
     3361 gcggaacgct gcttgaggat cgcaacctga gctacagtgt tcaggaaggc tacgccagca
     3421 acggtgtggg taacagcggt aacgctaacg ttggctatca gggtgggtcc ggtaatgtca
     3481 acgtaggcta tagctacggg aaagattacc ggcagctcaa ctacagcgtt cgcggcggcg
     3541 tgatagttca tagcgaaggc gtgacgcttt cccaaccgct aggcgaaacc atgacgctca
     3601 tctccgtacc cggtgcgcgc aatgcccgcg tggtgaataa cggcggcgtt caggttgact
     3661 ggatgggtaa cgcgatcgtg ccttacgcca tgccgtatcg tgaaaacgaa atctcactgc
     3721 gtagcgattc gttgggtgac gatgttgacg ttgaaaatgc gttccagaaa gtggtgccaa
     3781 cgcgtggagc gattgtcaga gcgcgttttg atacccgcgt tggttaccgc gtattaatga
     3841 cgctgcttcg ttccgcgggc agcccggtgc cctttggagc aacggcaacg ctaatcaccg
     3901 ataaacaaaa cgaggtgagc agtatcgttg gtgaagaagg acagctctat attagcggaa
     3961 tgccagagga aggacgggta ttgattaaat ggggtaatga cgcgtcgcag caatgcgtgg
     4021 cgccttataa attatccctg gaattaaaac agggcggaat tattcctgtt tcggccaatt
     4081 gccagtaagc aaggagagcg aaatgaaagc ggcaatagca ttatccttat taggatgcgt
     4141 attcggattt tctggcaagg catttgccgg agatgcgtgg gggccatgta cccccgcgga
     4201 tggcacgact tatcactata atgtcgatgt ggatgttggt atacctgatg cggcaaaaaa
     4261 cgtcgcagga acggttttac cggatgtcct taactggtct aatggacaaa acgtatcgct
     4321 aatttgtgaa tgtcctgatt cttataagaa tgaaaaagat actctagtac aaggggtgag
     4381 tatgcttccc ccttctggcc gttcggttga cagtatgaaa tattatactt tgacggagga
     4441 actggaggtt gcgacgaaaa ttcgcatcag taccagtgtg tatggatttg ttcctttcaa
     4501 aaaccagcag gcgctgcaaa caaccggatg caacaaagtc attactacgc cctatatggg
     4561 cggcgcaggt ctactttcct ttgctattac taaacccttt atcggcgatt ccgtcatccc
     4621 tctgacatta attgccgaac tgtatgcctc caaaacaaat aaagattacg gaacaatacc
     4681 catatcttcc gtatctattc agggccgcgt caccataact caggattgcg aaataaagcc
     4741 cggcacggtg ctggatgtgc catttggtga attcccctcc tcagcgttta aaaacagaca
     4801 agggcaaatg cctgaaggtg cgacagagca agaaattaac ctcggctttg attgcaacaa
     4861 tatctcggat ggtatcaaag tctcgttgcg ccttgagggg gcaaccaatg ctgacgatcc
     4921 ccgagcggtg gatatgggca accccgatat tggcgtactg gtgaaagatt ccagcggcaa
     4981 aattttggta ccgaatgatg caagcagcac cacgttatta aatttgagca gcctggattc
     5041 taaaactcac cgcaatgccg cgattcgact gctggcttta ccgattagca cgacgggtaa
     5101 agcgcctaaa ggcgggacgt ttgaaggcgt aacgaccata tatcttgaaa tggagtgatg
     5161 ccatgaaatt caatctattg ctgcatagcg gcatcgccag tttgagtctg tttgcctgcg
     5221 gggtgaatgc ggcgacggat cttggcccgg caggggatat tcatttctcc atcactatca
     5281 ccactaaagc ttgcgagatg gaaaaaagcg atctcgaagt cgatatggga acaatgacgc
     5341 tgcaaaaacc tgcggcagtc ggtacggtgt tgagcaagaa agatttcacc attgaactca
     5401 aagagtgcga tgggatatcc aaagcgaccg ttgagatgga cagtcagtcg gacagcgatg
     5461 atgattccat gtttgccctt gaggctggtg gcgcaacggg tgttgcgttg aagatagagg
     5521 acgataaagg aacgcagcaa gttcccaaag gctccagcgg aacgccgatt gaatgggcga
     5581 ttgatggcga aaccacgtcg cttcactacc aggcgagtta tgtggtcgtc aacactcagg
     5641 ccactggtgg cacagcgaat gcccttgtca atttttccat cacctatgag taagttactg
     5701 ggccagcatc ctggcccaac ctatctctga atagtcgtta atccgttaaa atggtctatt
     5761 ccggacttgc acttttgtaa atttgtgctt catgcacact ctttccccac actttttccc
     5821 tttgctgtgg tctacttatt cgcgcgtgta gattttactt atctgactac ctccgcactt
     5881 ttcccctgct gggcctgaaa agccgctaag cagggtgtta tcacctgttt gtccagggtt
     5941 tgtttgcatg agatacatca aatcgattac acagcagaag ctgagctttt tgcttgcaat
     6001 ctatattggc ctttttatga atggcgcggt tttttaccgc cgcttcggca gctatgcgca
     6061 cgattttacc gtctggaaag gcatttctgc tgttgttgaa ctggccgcca ccgtactggt
     6121 gaccttcttt ttactacgtc ttctttcgct gtttggccgc cgcagttggc gtattctggc
     6181 atcgctggtt gtgctctttt ccgcaggtgc cagctattac atgaccttcc ttaatgtggt
     6241 cattggttat ggcatcatcg cttccgtcat gaccaccgat atcgacctgt caaaagaagt
     6301 tgttggtctg aactttattc tctggttaat cgccgttagc gcattgcctc ttatccttat
     6361 ctggaataac cgctgtcgct acaccttgct ccgacaactg cgaaccccag ggcagcgtat
     6421 tcgcagcctg gcggtcgtcg tactggcggg aattatggtt tgggcaccga ttcgtttgct
     6481 ggatatccag cagaagaaag tggagagggc gaccggcgtt gatttgccga gttatggcgg
     6541 tgtcgtagcg aactcttatc tgccatcaaa ctggctttct gcgttggggc tgtacgcctg
     6601 ggcgcgggtc gatgaatctt ccgataataa ttcattgctt aacccggcga agaaattcac
     6661 ttatcaggca ccgcaaaatg ttaatgacac ttatgtcgtg tttatcatcg gtgaaactac
     6721 gcgttgggac catatgggca ttttcggcta tgagcgtaat accacgccga agctggccca
     6781 ggagaaaaat ctggcggcgt tccgtggtta ctcctgtgat accgcaacca aactctctct
     6841 gcgttgcatg tttgtacgtc aggggggcgc ggaagataat ccgcagcgca cattaaaaga
     6901 acagaacatt ttcgcggtac tgaagcagtt aggattcagt tctgacctct acgctatgca
     6961 gagcgaaatg tggttctaca gcaatacgat ggcggacaat attgcttacc gtgaacagat
     7021 tggtgcggag ccacgtaacc gtggcaagcc ggtagatgat atgttgctgg tagacgaaat
     7081 gcagcaatcg ctggggcgca acccggatgg taagcatcta atcattctgc ataccaaagg
     7141 ttcgcacttt aactacaccc agcgttaccc gcgcagcttt gcgcagtgga agccggaatg
     7201 tattggtgtt gatagcggat gtaccaaagc gcagatgatc aactcctatg acaactcggt
     7261 gacctatgtg gatcacttta tctccagtgt gattgatcag gttcgcgata agaaagcgat
     7321 tgtgttctac gcagctgacc acggcgagtc aattaatgaa cgcgagcacc tgcacggcac
     7381 gccgcgtgaa ctggcaccgc cggagcagtt ccgcgtaccg atgatggtct ggatgtcaga
     7441 taaatatctg gaaaatccgg tcaatgcgca ggcgtttgcg cagctgaaaa aagcagccga
     7501 catgaaagtg ccacgccgtc acgtagagct gtacgacacc atcatgggtt gtcttggcta
     7561 tacttcaccg gatggtggaa ttaacgaaaa caacaactgg tgtcacatcc cgcagacaaa
     7621 agaggcagtg gctaactaag aggcctgctg actttctcgc cgatcaaaag gcattttgct
     7681 attaagggat tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggtgattgg
     7741 cgcagcctgg tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctatca
     7801 ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt ttgcatctgc agcccatgag
     7861 gaagagaacc tccgggggca gggaggttcg actcgacgta ggcctgataa gacgcgtcag
     7921 cgtcgcatca ggcaacggca ccgcacttat tgtcggatgc ggcgtgaacg ccttattcga
     7981 cctacacacc acacatttcc ctgccaaatt tgcgccccat atctcactat ccattaacgg
     8041 atttgtgaca gaaaccattg tcttttttta tatttgctta aatctttttt tgcgttactt
     8101 atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt catggtatta atcgctcaga
     8161 tatccacctt ttcctcagat ttttttcatt aaatgcataa aacttaatca ccaaatgttg
     8221 ctaaatatta agctgattgt tctggtcatg ttggagtagc acaaatttct ctgctgcaaa
     8281 tagctgtttg aagttttttt aatctttgtt tgtggattct caccgttggt gtaaatcccg
     8341 gttggctgta ttgacgtttt cacattctgt tgacagattg taggtcacga ggggcatttt
     8401 atggaggatc cgcactgtta cactgatgtt aattagtacg gcatccccac ctcataacgt
     8461 tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga caacccactg caaagggtta
     8521 aaacaacaaa catcacaatt ggagcagaat aatgcgtatt tccttgaaaa agtcagggat
     8581 gctgaagctt ggtctcagcc tggtggctat gaccgtcgca gcaagtgttc aggctaaaac
     8641 tctggtttat tgctcagaag gatctccgga agggtttaac ccgcagctgt ttacctccgg
     8701 taccacctat gacgcctctt ccgtaccgct ttataaccgc ctggttgaat ttaaaatcgg
     8761 caccaccgaa gtgatcccgg gcctcgctga aaagtgggaa gtcagcgaag acggtaaaac
     8821 ctataccttc catctgcgta aaggtgtgaa gtggcacgac aataaagaat tcaaaccgac
     8881 gcgcgaaatg aatgccgatg acgtggtgtt ctcgttcgat cgtcagaaaa acgcgcaaaa
     8941 cccgtaccat aaagtttctg gcggtagcta cgaatacttc gaaggcatgg gcttgccgga
     9001 gctgatcagc gaagtgaaaa aggtggacga caacacagtt cagttcgtgc tgactcgccc
     9061 ggaagcgccg ttcctcgctg acctggcaat ggacttcgcc tctattctgt caaaagaata
     9121 tgctgacgcg atgatgaaag ccggtacacc ggaaaaactg gacctcaacc caatcggaac
     9181 cggtccgttc cagttacagc agtaccaaaa agattcccgt atccgctaca aagcgtttga
     9241 tggctactgg ggcaccaaac cgcagatcga tacgctggtt ttctctatta cccctgatgc
     9301 ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag gtgatgccgt acccgaaccc
     9361 ggcagatatc gctcgcatga agcaggataa atccatcaac ctgatggaaa tgccggggct
     9421 gaacgtcggt tatctctcgt ataacgtgca gaaaaaacca ctcgatgacg tgaaagttcg
     9481 ccaggctctg acctacgcgg tgaacaaaga tgccatcatt aaagcggttt atcagggcgc
     9541 gggcgtggca gcgaaaaacc tgatcccgcc aaccatgtgg ggctataacg acgacgttca
     9601 ggactacacc tacgatcctg aaaaagcgaa agccttgctg aaagaagcgg gtctggaaaa
     9661 aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt ccgtataacc cgaacgctcg
     9721 ccgcatggcg gagatgattc aggcagactg ggcgaaagtg ggcgtgcagg ccaaaatcgt
     9781 cacctacgaa tggggtgagt atctcaagcg tgcgaaagat ggcgagcatc agacggtaat
     9841 gatgggctgg actggcgata acggggatcc ggataacttc ttcgccaccc tgttcagctg
     9901 cgccgcctct gaacaaggct ccaactactc aaaatggtgc tacaaaccgt ttgaagatct
     9961 gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc gttgaactgt acaagcaagc
    10021 gcaggtggtg atgcacgatc aggctccggc actgatcatc gctcactcca ccgtgtttga
    10081 accggtacgc aaagaagtta aaggctatgt ggttgatcca ttaggcaaac atcacttcga
    10141 aaacgtctct atcgaataat taaaagccat acaagactga tggcaaaggc aaaactgcct
    10201 gatgcgctcc gcttatcagg cctacgaaaa ttctgcaatg tattgagttt gcaagatttt
    10261 gtaggccgga taaggcgtta acgccgcatc ccgcataaac aaagcgctct ttgtcaacaa
    10321 tctgcatacc cggtggtgct gtgcctctgc gtgcagcgcc atccggcagc aatacttcat
    10381 tccctgtcag acaagggcgg gaattgattt gtgagcaata cagacacgca gttccaggct
    10441 gcgggtcact acagagaatc cgggttatgt tgcagtttat tctccgacgt ttgggactcg
    10501 tcatccccac gtttatcggt attacccttc tcacatttgc ctttgtccac atgatcccgg
    10561 gcgatccggt gatgatcatg gcgggcgagc gtgggatctc cccagagcgt cacgcgcagc
    10621 tgctggctga actcggctta gataaaccga tgtggcagca gtatctccat tacatttggg
    10681 gcgttatgca tggcgatcta ggcatttcaa tgaaaagccg catcccggtt tgggaagagt
    10741 tcgtgccgcg cttccaggcc acgctggaac ttggcgtctg cgcgatgatt tttgctacgg
    10801 cagtcggtat tccggttggt gtgctggctg cggttaaacg cggttccatt ttcgatcaca
    10861 cagcggttgg cctggcgctg acaggttatt caatgcctat cttctggtgg ggcatgatgc
    10921 tgatcatgct ggtttcggtg cactggaacc tgacacccgt ctccggtcgc gtgagcgata
    10981 tggtgttcct cgatgattcc aatccgttaa ccggttttat gctgatcgac accgccatct
    11041 ggggtgaaga cggtaacttt atcgatgccg tcgcccatat gatcttgccc gccattgtgc
    11101 tgggtactat tccgctggcg gtcattgtac gtatgacacg ctcctcgatg ctggaagtgc
    11161 tgggtgaaga ttacatccgc accgcgcgcg ccaaaggatt gacccgcctt agggtgatta
    11221 tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac cgttatcggc ctccaggtgg
    11281 gaacattgct ggcgggggcg attctgaccg aaaccatctt ctcgtggccc ggtctggggc
    11341 gctggttgat tgacgcactg caacgccgcg actatccggt agtgcagggc ggcgtattgc
    11401 tggtggcgac gatgattatc ctcgtcaatc tgctggtcga tctgctgtac ggcgtggtga
    11461 acccgcgtat tcgtcataag aagtaagggg acatcatgtc acaggttact gaaaataaag
    11521 tgattagcgc accggtgccg atgaccccgt tacaggagtt ctggcactat tttaaacgca
    11581 acaaaggcgc ggtcgtcggg ctggtttacg tcgtcatcgt gctgtttatc gcgatctttg
    11641 ccaactggat tgctccctat aacccggcgg aacaattccg cgatgcgctg cttgccccgc
    11701 ccgcctggca ggaaggcggc agcatggcgc acttgctggg caccgatgac gtaggccgtg
    11761 atgtgctgtc gcggctgatg tacggcgcgc gtttatcgct gctggttggc tgtctggtgg
    11821 tggtgttatc gctgattatg ggcgttattc tcggcctgat cgccggttac tttggcggcc
    11881 tggtcgataa catcatcatg cgcgtggtcg atatcatgct ggcgctgcca agtctgctgt
    11941 tggcactggt gctggtggcg attttcggcc cgtcgattgg taacgccgcg ctggcgctga
    12001 ccttcgttgc cttgccgcac tatgtgcgct taacacgcgc cgccgtgctg gtggaggtga
    12061 atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg ggcgatgcgt cagatgttta
    12121 ttaacatctt cccgaactgc cttgcgcctc tgattgttca ggcgtcgctc ggtttctcta
    12181 acgccattct cgatatggct gctcttggtt tcctcggcat gggggcgcag ccgccaacgc
    12241 cggagtgggg caccatgctc tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg
    12301 tgaccttccc gggtctggcg atcctgctga cggtgctggc atttaacctg atgggtgacg
    12361 gtctgcgtga cgcgctcgat cccaaactga agcagtaaga ggttcgagat ggcgttatta
    12421 aatgtagata aattatcggt gcatttcggc gacgaaagcg caccgttccg cgccgtagac
    12481 cgcattagct acagcgtgaa acagggcgaa gtggtcggga ttgtgggtga gtccggctcc
    12541 ggtaagtcgg tcagttcact ggcgattatg gggctgattg attatccggg ccgcgtgatg
    12601 gcggaaaagc tggagtttaa cggtcaggat ttgcagcgta tctcagaaaa agagcgccgc
    12661 aatctggtgg gtgccgaagt ggcgatgatc ttccaggacc cgatgaccag ccttaacccg
    12721 tgctacaccg ttggtttcca gattatggaa gcgattaagg tgcatcaggg cggtaataag
    12781 agcactcgcc gtcagcgggc gattgacctg ctgaatcagg tcggtattcc cgatccggca
    12841 tcgcgtctgg atgtttaccc gcatcagctt tccggcggca tgagccagcg cgtgatgatt
    12901 gcgatggcca ttgcctgtcg gccaaaactg ctgattgccg atgaaccgac cactgcgctg
    12961 gacgtgacca ttcaggcgca aatcatcgaa ctactgctgg agctacagca gaaagagaac
    13021 atggcgctgg tgttaattac ccatgacctg gcgctggtgg cggaagcggc acataaaatc
    13081 atcgtgatgt atgccggtca ggtggtggaa acgggcgatg cgcacgccat cttccatgcg
    13141 ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc cggaatttgc tcaggacaaa
    13201 gaacgtctgg catcgttgcc tggcgttgtt cccggcaagt acgaccgccc gaacggctgc
    13261 ctgcttaacc cgcgctgccc ctatgccact gacagatgtc gcgctgaaga accggcgctg
    13321 aatatgctcg ctgacgggcg tcagtccaaa tgccattacc cacttgatga tgccgggagg
    13381 ccgacactat gagtacgcaa gaggccacct cgcaacaacc gctgttgcag gctatcgacc
    13441 tgaaaaaaca ttatccggta aagaaaggta tgttcgctcc ggaacgtctg gtgaaggcgc
    13501 tggacggcgt ttcgtttaac cttgaacgtg gcaaaacgtt ggcggtagta ggtgaatctg
    13561 gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat tgaaacgccc accggcggcg
    13621 agctgtatta ccaggggcag gatctgctta agcacgatcc gcaggcgcag aagctgcgtc
    13681 ggcagaaaat ccagattgtc ttccagaacc cttacggttc gctgaatccg cgtaaaaaag
    13741 tcgggcaaat tcttgaagag ccgctgctga tcaacaccag cttaagcaaa gaacagcgtc
    13801 gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa aaccgagcac tatgaccgct
    13861 atccgcatat gttctccggc ggacaacgtc agcgtatcgc tattgcccgt ggtctgatgc
    13921 tcgacccgga cgtggtgatt gccgatgaac cggtctccgc gctggacgtc tcggtgcgtg
    13981 cacaggtgct caacctgatg atggatttgc agcaggaact ggggctttct tatgtcttca
    14041 tctcccacga cctgtcggtg gtggagcaca ttgctgatga agtgatggtg atgtacctgg
    14101 gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa taacccgcgc catccgtaca
    14161 ctcaggcgct actttccgcg acgccgcgcc tgaatccgga cgatcgccgc gagcgcatca
    14221 aactcaccgg tgaactgcca agcccgctga atccaccgcc gggctgcgcg tttaacgccc
    14281 gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc gcagctaaaa gactacggcg
    14341 gtcaactggt agcttgtttt gctgttgatc aggatgaaaa tccgcagcgt taactcacaa
    14401 aaccggggcc atgcgctccg gtttactcaa tcagcttcaa cagtggcgac aagcatagca
    14461 aaatgccgta aagcagaatc agccaggctt taaatccgcg aagtttttcc agttgtgcga
    14521 ctttatagat caggaaaaac ggaatgaggc acgacacaat gccatataac gggcttccca
    14581 actgaaagaa caccagcacc gatacacgaa acgaaaccca aatcgtcaac gtaatgacga
    14641 taaaagcgca gatcgccaga gtcagcacgc gtgagttaat tttcttggta tcaataattc
    14701 ggcttaacag attgagaata atgcctttaa tggcctcgtg gaaaccgaga taaatgccaa
    14761 agaatgcggt cagtacggca aagatattca gcaccgtaga ggtgatatga atgatatgcc
    14821 cagggatcac ctgcgcggcc agtgccagtg ccgagatatt ttgttcaaag gcagaaacgg
    14881 cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa aaacagtatc accgcgatga
    14941 gcgtgatata actaattcgg tgggtacgca gcgcgagccg ggttgccagt actttatccg
    15001 cttcccgttt acgataggca atattcattg ggttaagtac ctgaataaat actgcagaaa
    15061 agaagcaaaa tggaatggta agcaggacat cgcgaaaaaa gacggaggct tgcgggaagg
    15121 cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa accgaacacg acaataatcc
    15181 ctactttgac caccaccatt ggcccagaaa tcttaaacag taatcgttca ccaccagacg
    15241 caatcgctac cagtacggcg aaaatagcga ctttataaag tagagattgt gaaagatcgg
    15301 catcggttaa accgaaggtt ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt
    15361 agataaaaat cccgtggata atcatcaaaa agtagataac cccgaggaaa attccccagt
    15421 tcttccccag gtaatgactg ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa
    15481 gggtttttaa ataaatgtcc tgcactaccc aggtggcagg ataagcaatg atcgctgcgg
    15541 taataaatac ccaaataccc ttcaagccaa tttgtactgg catcagcacg gttccggcac
    15601 caatcgccat gcctatgcat aataaaaccc agccgaagtc gtagcgtgta aacggcagtt
    15661 tctgatcgtg tttcggtagt gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc
    15721 ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag gtggagaagt cgaatacgca
    15781 gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg cgaggaggcg caattctaca
    15841 aaattgcgca tttcactacc agtctggagt cagcccggat atgaactgca actattgcaa
    15901 ataaagaaat taagctggaa ggcatcgcgc aatgaggaag attgccgggt gctgacagta
    15961 aaaagcaatc gtaaaatcag cggcaagccg gaagggttcc ggtgaggcgc aatgttgcgg
    16021 gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta
    16081 tgcacctgac aacataacgg gggctaatct tgaacctaga ccactcaaga atagccgcga
    16141 aacgttgtca ttacaacaca ggcggctata tgacgctcgc agagctgggc atggccttct
    16201 ggcatgattt agtggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc
    16261 tgaacaagcg gaagtaatgt gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc
    16321 cggaccaggc cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg
    16381 caagcggata atattctgcg gaatgcgcag cacagagtga agacgtgacg gattccggaa
    16441 cagaaaaaca gtcgtagaat cagcgtcaag ccggaaggat tccggtgagg cgcaatgttg
    16501 cgggggctta atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag
    16561 gtatgcacct gacaacatca cgggggccat tcttgaacct gaacaactca agaatagccg
    16621 cgaaacgttg tcattacaac acaggcggct atatgacgct cgcagaactg ggcatggcct
    16681 tctggcacga tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact
    16741 ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc
    16801 gcccggacca ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga
    16861 ttgcaagcgg ataatattct gcagaatgcg cagcacagag tgaagacgtg acggattccg
    16921 gaacagaaaa acagtcgtag aatcagcgtc aagccggaag gattccggtg aggcgcaatg
    16981 ttgcgggggc ttaatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg
    17041 caggtatgca cctgacaaca tcacgggggc cattcttgca tctagaccat tcaagaatag
    17101 ccgcgaaacg ttgtcattac aacacaggcg gctatatgac gctcgcagag ctgggcatgg
    17161 ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt atgatcgtga
    17221 actggctgaa caagcggaag taacgtgtca gacgggcgtc aggctaccgt aatggcaatt
    17281 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtgct tactgcgggt
    17341 aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt atcctggtat tgaataacta
    17401 ctgcatttga gttctcggag accggtgctg tttgtggcaa cccactggtg agttttttcc
    17461 agtcaacatt gtcttcggtg aaaatcttgc catcaagaac gcgaaccacc agatcggaga
    17521 tagccaggaa gctgctcggt tggtcgatga caatcggtgc cccctgatgt ggtgccttca
    17581 tgccgaagaa tttcacccca acggggacgt cggtgataga cgggctaggg atatcacgta
    17641 ggccagatac ctgcattctg tcgcccttca gcgcgccacc gtgttccggc accacgacca
    17701 ccatcacttt acgacccgat ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga
    17761 atttctgcgc ccgcgctttg taatccgctg ttttgctgac gcccggataa tggttgccgt
    17821 catgcagtgg aagcgtgttg tagaacgtgg cactacggct gtttttatct ttttcggtaa
    17881 cgtccagcca gcggttaagc acggcggtat cgtcataaac cggcgaacca tcaaagccca
    17941 gcaaaataac cggcagattt gtttgatcca tcaattcgct ctgcatgccg ccattttcgc
    18001 gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc catcatcagg tgctgggtaa
    18061 agcccagttt cgacaggtta tcaaacagat agcagtcgtt atttgccggt tgatacagat
    18121 tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg gatcgccgcc gggccactgt
    18181 aggaggtggc ggagttaaag ttcttgaact caatatcgaa atgcgaccac agcggatgcg
    18241 acatcaaccc ggcggcttca atatccgacc aggaaagcga acaaatatta atcaccagta
    18301 gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa gatcgattta cgtttcgcct
    18361 ccgcgttata gaaattattc agccaggcgt taaggttcgc cgttgttggc ggtgcagttt
    18421 gtgcgggcat atcacccact accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt
    18481 taccacccgt cgttgttaca gtggtcgtcg gttgaccggc tggccacaag gagaaacttg
    18541 gtcccgccag ggtaagtacg ttcagccaca gcagaatggc aaccacaaaa acggtaatgc
    18601 gaatccattg tgacaggaat aaccaggcca ctaataaaac aaaaatggcc ccaatcatct
    18661 gccagttaat aaagcgtgtg acaaggtcga ttaaataatc ggtactgaac cccgccacct
    18721 gcgaaccctg gctcattatg ctttccgggc caggcaacca ggtgtcatgc cagaacaaag
    18781 caaagccgat cggcagggca atccagtggc gcaagcgatg caggctgtag cgcggaatgg
    18841 gcatcagcag aaacgcggca aacaccaaat tgaggagcgg atggaagtta agatatcccg
    18901 cccacaacag gccgaactta accagaaaat agaagttcca gccggaaagg ccgcgccagt
    18961 attgccagag ggaagaaggc atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc
    19021 cctggctttt tccgtgcggc gtaacgtccc agccggttta acataacgag gtttagcaaa
    19081 gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg
    19141 gaaaaatatc agtgcgcaaa cgacaataat ttcaatgata tcgctgatgg tcatcatgat
    19201 gagcgctcca cagcatcatc taacagtcgc atgggttctg gtattcgtcg ccagtggcga
    19261 ccatcgtgct cggcattgat gaccggttta gaactttgtg ttaaaggcag cggcatgccc
    19321 cattgttctg gggcaagcag gcgcatctgc accagctcgg cactgatttg atcatcttca
    19381 aaccagacca tacggtttga gaaaatgtcg ccagtaggca atgggaaaat atgattcaac
    19441 gcggtatcca gatcgttaat ccgacagaat gagagaaaca gcaccagccg attaccgcca
    19501 atggtcatga tatcgccggt acggttaggg cgacatagcg tcagggcttg ctcaacgcgg
    19561 atacccggta ccggacgtag ggcaaccaga acacctttac cgtgggcagg caaaagaggg
    19621 ttattcatca tgttgttgac ggcattacag aacacatccc acttctggaa accacgcagt
    19681 ttgagcggct gggtcattga cagcaaggta gtgatatctt ccggcacata gcgactaaac
    19741 ttctgccctt gcacgctttc gatcatcgtc agacagcggg agagtggcgc attccacggg
    19801 ataaccatat ttgcaccgca ggccaataac aaacgttcat cggtggcgcg caggctagcg
    19861 gtattttccc gtacgaggat tttcatcgca ctaccgcgct ggcgacgcag agtatgaatg
    19921 ctgcgggcca gtggctcgat ttgcgcattt tgttgtaaag aaaagaccac cgtcgccgcc
    19981 tgagcggtac gggcttcatt gaacaggact tcgttattgt tgaacagttg ccagtgttcc
    20041 gatagcggcg gcgcaccttc cagtacagca acattactca gaatgcgttt ttcgtcgctg
    20101 cgtggttgga tctccgcctc ttcgctttga actaatgtcc agataccatt ttgttgctga
    20161 acgctaagct gctgacgggc gctgacccct ttttcgttgc accagaaggc aatatccagc
    20221 aaatgttggt cgccctgaaa acgcaaactg gcaagaccaa aaagtgaacg gtattcctca
    20281 agcaacaatg aaaattgttt atcgttatta tttccgggat taattaccaa aagcgaacaa
    20341 tggtttaacc tgctccattt attcatttta tccaaccatg agcgaagccg ctcggcagga
    20401 atgttttgcc atgcgttatt tgcgcaaaca agaataaaaa ggtaattatg gggatcaata
    20461 gaacactgca aatcacgggc caaatagtat agaccttttt catgatttag cattgaaaat
    20521 aattttattt tttccggccc ttgagaatca tctaattgaa agattttcgc cggatcgctg
    20581 tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc
    20641 agactgatag catcttcatg gcgatcgacg ttaaaccacc agacgccgcc tgctggcata
    20701 tgtcgcagct catcccataa tgatgagata ccgatagaga atacagggtc cacaatgtcc
    20761 ctcatgtcac cattttgtct gattatcagt ttactagcga aagctaagaa ataaacctac
    20821 cattgaaatt aaaacttatc agatttagca tgtgaatcaa tttagcaggt cacaagacgt
    20881 gtgattttag tcttttcgtt tttaactttt tctgttgcgt aacggaaagt caaaaagtga
    20941 gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat gctcacggtt agaattgtct
    21001 catgaacggt acggttattt catagggatc aagcaaaatg aataacaatg aaccagatac
    21061 tctgcctgat cccgcgatag gctatatctt ccagaatgat attttggcgt taaagcaggc
    21121 attttcactg cctgatattg attatgccga tatttcccaa cgcgaacagt tggccgcggc
    21181 attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg
    21241 gccgtactgg gattgcaggg ggtgcgggga ggcgtgggga caacaaccat caccgccgca
    21301 ttagcctggt cattacaaat gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac
    21361 aacttgttgc gcctgtcatt taacgttgat tttacccacc gtcagggctg ggccagagct
    21421 atgctggatg gccaggactg gcgtgacgct gggttgcgct acacctcgca gctcgatttg
    21481 ctgccttttg gtcagttatc cattgaagaa caagaaaatc cacagcactg gcaaactcgg
    21541 ctgagcgata tttgctccgg cttacagcaa ctaaaatcca gcgggcgtta ccagtggatt
    21601 ttaatcgact taccgcgtga tgcctcgcag ataacccacc agctgctgag tttgtgcgat
    21661 cactcgctgg caatcgtcaa tgtggatgcc aactgccata tccgactgca tcagcaagcg
    21721 ctgccggatg gcgcacatat tttgattaat gacttccgta ttggcagtca ggttcaggac
    21781 gatatttacc agctttggtt gcaaagccag cgccgattac tgccgatgct cattcatcgt
    21841 gatgaagcga tggctgaatg cctggcggct aagcaaccag taggtgaata tcgcagtgat
    21901 gcgctggccg ctgaagagat actgacgctg gcgaactggt gcctgttgaa ctattccggg
    21961 ctgaaaacgc cagtcgggag taaatcatga gtatcctgac ccggtggttg ctaatcccgc
    22021 cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt
    22081 tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt ccgctggagc
    22141 acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct gtatccgcat atcaacgcct
    22201 cgcgtccgcg tccgctggac ccggtccgtt atctcattca aacatgctgg ttactgatcg
    22261 gtacatcgcg caaagaaacg ccgaaaccgc gcaggcgggc attttcaggt ctgcagaata
    22321 ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc cataaaacac
    22381 agcatcttga tgagaaaaaa gagctcggtc atttgagtgc cggggcgcgg cggttgatcc
    22441 tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact cagccgttta
    22501 acccgctggc gcagtttatc ttcctgatgc tgctgtgggg ggtagcgctg atcgtacggc
    22561 ggatgccggg gcgcttctca gcgctaatgt tgattgtgct gtcgctgact gtttcttgcc
    22621 gttatatctg gtggcgttac acctctacgc tgaactggga cgatccggtc agcctggtgt
    22681 gcgggcttat tctgctcttc gccgaaacgt acgcgtggat tgtgctggtg ctcggctact
    22741 tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaagat atgtcgctgt
    22801 ggccgtcggt ggatatcttt gtcccgactt acaacgaaga tctcaacgtg gtgaaaaata
    22861 ccatttacgc ctcgctgggt atcgactggc cgaaagacaa actgaacatc tggatcctcg
    22921 atgacggcgg cagggaagag tttcgccagt ttgcgcaaaa cgtgggggtg aaatatatcg
    22981 cccgcaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg aaatatgcca
    23041 aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg ttcctgcaaa
    23101 tgaccatggg ctggttcctg aaagagaaac agctggcgat gatgcaaacc ccacaccatt
    23161 tcttctcgcc ggacccgttt gaacgcaacc tggggcgttt tcgtaaaaca ccgaacgaag
    23221 gcacgctgtt ctatggtctg gtgcaggatg gcaacgatat gtgggacgcc actttcttct
    23281 gcggttcctg tgcggtgatt cgccgtaagc cgctggatga aattggcggc attgctgtcg
    23341 aaaccgtgac tgaagatgcg catacctctt tgcgcctgca ccgtcgtggc tacacctctg
    23401 catatatgcg tattccgcag gcggcggggc tggcgaccga aagtctgtcg gcgcatatcg
    23461 gtcagcgtat tcgctgggcg cgcgggatgg tgcaaatctt ccgtctcgat aacccgctca
    23521 ccggtaaagg gctgaagttt gctcagcggc tatgttacgt caacgccatg ttccacttct
    23581 tgtcgggcat tccacggctg atcttcctga ctgcgccgct ggcgttcctg ctgcttcatg
    23641 cctacatcat ctatgcgcca gcgttgatga tcgccctgtt cgtgctgccg catatgatcc
    23701 atgccagcct gaccaactcc aagatccagg gcaaatatcg ccactctttc tggagtgaaa
    23761 tctacgaaac ggtgctggcg tggtatatcg caccaccgac gctggtggcg ctgattaacc
    23821 cgcacaaagg caaatttaac gtcaccgcca aaggtggact ggtggaagaa gagtacgtcg
    23881 actgggtgat ctcgcggccc tacatcttcc ttgttctgct caacctggtg ggcgttgcgg
    23941 taggcatctg gcgctacttc tatggcccgc caaccgagat gctcaccgtg gtcgtcagta
    24001 tggtgtgggt attctacaac ctgattgttc ttggcggcgc agttgcggta tcggtagaaa
    24061 gcaaacaggt acgccgatcg caccgcgtgg agatgacgat gcccgcggca attgcccgcg
    24121 aagatggtca cctcttctcg tgtaccgttc aggatttctc cgacggtggt ttggggatca
    24181 agatcaacgg tcaggcgcag attctggaag ggcagaaagt gaatctgttg cttaaacgcg
    24241 gtcagcagga atacgtcttc ccgacccagg tggcgcgcgt gatgggtaat gaagttgggc
    24301 tgaaattaat gccgctcacc acccagcaac atatcgattt tgtgcagtgt acgtttgccc
    24361 gtgcggatac atgggcgctc tggcaggaca gctatccgga agataagccg ctggaaagtc
    24421 tgctggatat tctgaagctc ggcttccgtg gctaccgcca tctggcggag tttgcgcctt
    24481 cttcggtgaa gggcatattc cgtgtgctga cttctctggt ttcctgggtt gtatcgttta
    24541 ttccgcgccg cccggagcgg agcgaaacgg cacaaccatc ggatcaggct ttggctcaac
    24601 aatgatgata acgcgatgaa aagaaaacta ttctggattt gtgcagtggc tatggggatg
    24661 agtgcgttcc cctctttcat gacgcaggcg acgccagcaa cgcaaccact gatcaatgct
    24721 gagccagcta tagccgccca gacggaacaa aatccgcagg tggggcaagt gatgccgggc
    24781 gtgcagggcg ctgatgcgcc agtcgtggcg cagaacggtc cttcgcgtga tgtgaagctg
    24841 acctttgcgc aaattgcgcc gccgccgggc agcatggtgc tacgtggcat taacccgaac
    24901 ggcagcattg agtttggtat gcgcagcgat gaagtggtga cgaaggcgat gctcaacctc
    24961 gaatacacgc catcgccatc gttactgcct gtccagtcgc agttaaaggt ttatctcaat
    25021 gatgagctga tgggcgtgct gccagtgacc aaagaacagt tgggtaaaaa aacgctggcg
    25081 caaatgccca ttaacccact gtttattacc gacttcaacc gtgtgcggct ggagtttgtc
    25141 ggccattatc aggacgtgtg cgaaaacccg gccagcacca cgctttggct ggatgttggg
    25201 cggagcagtg gactggattt gacctatcag accctgaatg tgaagaatga cctgtcacac
    25261 ttcccggtac cattctttga cccgcgtgat aaccgcacca acaccttgcc gatggtcttt
    25321 gcgggtgcgc cggatgttgg gctgcaacaa gcatctgcca ttgtcgcctc gtggtttggt
    25381 tcgcgttctg gctggcgtgg gcagaacttc ccggtgctct ataaccaact gccggatcgc
    25441 aatgccattg tctttgcaac caacgacaaa cggccggact tcctgcgcga tcatccggcg
    25501 gtaaaagccc cggtgattga gatgattaac catccgcaga atccttacgt caaactgctg
    25561 gtggtgtttg gtcgtgacga caaagacctg ttgcaggcag cgaaaggtat cgctcagggt
    25621 aacattctgt tccgtggtga aagcgtggta gtgaatgaag tgaaaccgct gctaccgcgt
    25681 aagccgtacg atgcgccgaa ctgggtacgt accgatcgtc cggtcacatt tggcgaactg
    25741 aaaacctatg aagaacagtt acaatccagc ggtcttgagc cagcagcgat taacgtttcg
    25801 ctaaacctgc cgccggatct ctacctgatg cgcagtaccg gcattgatat ggatattaat
    25861 taccgctaca ccatgccgcc ggtgaaagac agttcgcgga tggatatcag cctgaataac
    25921 cagttcctgc aatccttcaa cctgagcagt aaacaggagg cgaaccgcct gctgcttcgg
    25981 attccggtat tacaaggttt gctggatggc aaaacggatg tctctattcc ggcgctgaaa
    26041 ctgggcgcga ccaaccagct gcgcttcgac tttgagtata tgaacccgat gccgggcggt
    26101 tcggtggata actgtattac cttccagccg gtgcagaatc atgtggtgat tggtgacgat
    26161 tccaccatcg acttctcgaa gtattaccac ttcatcccga tgccggatct acgcgccttt
    26221 gctaacgcgg gcttcccgtt cagccggatg gcggatctgt cgcaaaccat caccgtgatg
    26281 ccgaaaacgc ctaacgaagc acagatggaa acgttgctga atactgttgg ttttatcggc
    26341 gcacagacgg gcttcccggc gattaatctg acggtgaccg atgatggcag caccattcag
    26401 ggcaaagatg ccgacatcat gatcatcggt ggtatcccgg acaaactgaa agacgataag
    26461 cagatcgacc tgttggtgca ggcgaccgaa agctgggtga aaacgccgat gcgccagacc
    26521 ccgttccccg gcattgtgcc ggacgagagc gatcgcgcgg cagaaaccca gtcaacgctg
    26581 acctcttccg gtgcgatggc ggcggtgatt ggcttccagt cgccgtataa cgaccagcgc
    26641 agcgtgattg cgctgctggc agatagccca cgcggttatg aaatgcttaa cgatgcggtg
    26701 aacgatagcg gcaaacgcgc caccatgttc ggttcggtcg cggtgatccg cgagtccggt
    26761 atcaacagcc tacgtgttgg cgacgtttat tacgtaggtc atctgccgtg gttcgagcgc
    26821 ttgtggtatg cgctggcaaa ccatccgatt ctgctggcgg tgctggcggc tatcagtgtg
    26881 atattgctgg catgggtact gtggcgtctg ctgcgtatta ttagtcgtcg tcgtcttaac
    26941 ccggataacg agtaattgaa gatgaatgtg ttgcgtagtg gactcgtgac gatgctgctg
    27001 ttggctgcct ttagtgttca ggcagcctgt acctggcctg cctgggagca gtttaaaaag
    27061 gattacatca gtcaggaagg gcgcgtcatc gaccccagcg acgcgcgcaa aatcaccacc
    27121 tccgaagggc aaagttacgg catgttcttt gccctggcgg ctaacgaccg tgcagctttc
    27181 gataatattc tcgactggac gcagaacaat ctcgctcagg gttctttaaa agaacgtttg
    27241 cccgcctggc tgtggggcaa gaaagagaac agtaagtggg aagtgctgga cagcaattcg
    27301 gcctccgatg gtgatgtctg gatggcctgg tcgttgctgg aggcggggcg tttgtggaaa
    27361 gagcagcgtt ataccgacat cggcagcgcg ttgctaaaac gtattgcgcg ggaggaagtg
    27421 gtgacggtgc ctgggctggg ttccatgttg ttaccgggca aagtgggttt tgcagaggat
    27481 aacagctggc gttttaaccc cagctacctg ccgccgacgc tggcgcagta tttcacccgc
    27541 tttggcgcgc cgtggaccac gctgcgcgaa accaatcaac gtttattgct ggaaaccgcc
    27601 ccgaaaggct tttcgccaga ctgggtgcgc tatgagaaag acaaaggctg gcagctaaaa
    27661 gccgaaaaaa ccttgatcag cagctacgac gccatccgcg tttacatgtg ggtaggcatg
    27721 atgcctgaca gcgatccgca aaaagcgcgg atgctcaacc ggtttaaacc gatggcgaca
    27781 ttcactgaga aaaacggtta tccgccggaa aaagtggatg tggctacggg gaaagcgcag
    27841 ggtaaaggac cggtcggttt ttctgccgcc atgctgccct ttttacaaaa ccgtgatgcg
    27901 caggccgttc agcgccagcg cgtggccgat aactttcccg gcagcgatgc ctattacaac
    27961 tatgtgctga ccctgtttgg acaaggctgg gatcaacacc gtttccgctt ctcgacaaaa
    28021 ggtgagttat tacctgactg gggccaggaa tgcgcaaatt cacactaaac atattcacgc
    28081 tttccctcgg tctggccgtc atgccgatgg tcgaggcagc accaaccgct cagcaacagt
    28141 tgctggagca agttcggtta ggcgaagcga cccatcgtga agacctggtg cagcagtcgt
    28201 tgtatcggct ggaacttatt gatccgaata acccggacgt cgttgccgcc cgtttccgtt
    28261 ctttgttacg tcagggcgat attgatggcg cgcaaaaaca gctcgatcgg ctgtcgcagt
    28321 tagcgccgag ttcaaatgcg tataaatcgt cgcggactac gatgctactt tccacgccgg
    28381 atggtcgtca ggcactgcaa caggcaagat tgcaggcgac taccggtcat gcggaagaag
    28441 ctgtggcgag ttacaacaag ctgttcaacg gtgcgccgcc ggaaggtgac attgctgtcg
    28501 agtactggag tacggtggcg aaaattccgg ctcgccgtgg cgaagcgatt aatcagttaa
    28561 aacgcatcaa tgcggatgca ccgggcaata cgggcctgca aaacaatctg gcgctattgc
    28621 tgtttagtag cgatcgccgt gacgaaggtt ttgccgtcct ggaacagatg gcgaaatcga
    28681 acgccgggcg cgaaggggcc tctaaaatct ggtacgggca gattaaagac atgcccgtca
    28741 gcgatgccag cgtgcaggcg ctgaaaaaat atctttcgat ctttagtgat ggcgatagcg
    28801 tggcggctgc gcaatcgcaa ctggcagaac agcaaaaaca gctggccgat cctgctttcc
    28861 gcgctcgtgc gcaaggttta gcggcggtgg actctggtat ggcgggtaaa gccattcccg
    28921 aactacaaca ggcagtgcgg gcgaacccga aagacagtga ggctctgggg gcgctgggcc
    28981 aggcatattc acagaaaggc gatcgcgcca atgcagtggc gaatctggaa aaagccctcg
    29041 cactggaccc gcacagcagc aacaacgaca aatggaacag tctgctgaaa gtaaaccgct
    29101 actggctggc aatccagcag ggcgatgctg cgctgaaagc caataatcct gaccgggcag
    29161 aacgcctgtt ccagcaggcg cgtaatgtcg ataacaccga cagttatgca gtgctggggc
    29221 tgggcgatgt ggcgatggcg cgaaaagatt atcccgccgc cgaacgttat tatcagcaga
    29281 ccttgcgtat ggacagcggc aacactaacg ccgtgcgcgg gctggcaaat atttaccgcc
    29341 agcaatcgcc agaaaaagct gaagcgttta tcgcctcact ctctgccagt cagcggcgta
    29401 gcattgatga tatcgaacgc agcctgcaaa acgaccgtct ggcacagcag gcagaggcac
    29461 tggaaaacca gggcaaatgg gcgcaggcgg cagcacttca gcggcaacga ctggcgctgg
    29521 accccggcag cgtatggatt acttaccgac tttcgcagga tctctggcag gccggacaac
    29581 gcagccaggc cgatacgtta atgcgcaatc tggcgcagca gaagccgaac gacccggagc
    29641 aggtttacgc ttacgggctg tacctctctg gtcatgacca ggacagagcg gcgctggcgc
    29701 atatcaacag cctgccgcgt gcgcagtgga gcagcaatat tcaggagctg gttaatcgac
    29761 tgcaaagcga tcaggtgctg gaaaccgcta atcgcctgcg agaaagcggc aaagaggcag
    29821 aagcggaagc gatgctgcac cagcaaccgc cttcctcgcg tattgacctc acgctggctg
    29881 actgggcgca acaacgacgt gattacaccg ccgcccgcgc tgcgtatcag aatgtcctga
    29941 cgcgggagcc aactaacgcc gatgccattc ttggtctgac ggaagtggat attgctgccg
    30001 gtgacaaagc ggcggcacgt agccagctgg cgaaactgcc cgctaccgat aacgcctcgc
    30061 tgaacacaca gcggcgcgtg gcgctggcac aggcgcagct tggcgatacc gcagcggcgc
    30121 agcagacgtt taataagttg atcccgcagg caaaatctca gccaccgtcg atggaaagcg
    30181 cgatggtgct gcgcgacggt gcgaagtttg aagcgcaggc gggcgatcca aagcaggcgc
    30241 tggaaaccta caaagacgcc atggtcgcat ccggtgtgac tacgacgcgt ccgcaggata
    30301 acgacacctt tacccgactg acccgtaacg acgagaaaga tgactggctg aaacgcggcg
    30361 tgcgcagcga tgcggcggac ctctatcgcc agcaggatct taacgtcacc cttgagcatg
    30421 attattgggg atcgagcggc accggtggtt actccgatct gaaagcgcac actaccatgt
    30481 tgcaggtgga cgcgccgtat tctgacgggc ggatgttctt tcgcagtgat ttcgtcaata
    30541 tgaacgtcgg cagtttctcc actaatgccg atggtaaatg ggatgataac tggggcacct
    30601 gtacattaca ggactgtagc ggcaaccgca gccagtcgga ttccggtgcc agcgtggcgg
    30661 tcggctggcg aaatgacgtc tggagctggg atatcggcac cacgccgatg ggcttcaacg
    30721 tggtggatgt ggtcggcggc atcagttaca gcgatgatat cgggccgctg ggttacaccg
    30781 ttaacgccca ccgtcggccc atctccagtt ctttgctggc ctttggtggg caaaaagact
    30841 ccccgagcaa taccgggaaa aaatggggcg gtgtgcgtgc cgacggcgtg gggctaagcc
    30901 tgagctacga taaaggtgaa gcaaacggcg tctgggcatc gcttagtggc gaccagttaa
    30961 ccggtaaaaa tgtcgaagat aactggcgcg tgcgctggat gacgggctat tactataagg
    31021 tcatcaacca gaacaatcgc cgcgtcacaa tcggcctgaa caacatgatc tggcattacg
    31081 acaaagacct gagtggctac tcactcggtc agggcggtta ctacagtccg caggaatacc
    31141 tgtcgtttgc cataccggtg atgtggcggg agcgcacgga aaactggtcg tgggagctgg
    31201 gggcgtctgg ctcgtggtcg cattcgcgca ccaaaaccat gccgcggtat ccgctgatga
    31261 atctgatccc gactgactgg caggaagaag ctgcgcggca atccaacgat ggcggcagca
    31321 gtcagggctt tggctatacg gcgcgggcat tacttgagcg tcgcgtcaca tccaactggt
    31381 ttgtcggtac ggcaattgat atccagcagg cgaaagatta cgcgcccagc catttcctgc
    31441 tctatgtacg ttattccgcc gctggatggc agggtgacat ggatttaccg ccgcagccgc
    31501 tgatacccta cgccgactgg taagttttca gatagcgcct ctcttaatgc cgctgcgatc
    31561 gggtatactc gggcggcaat ctgggatttc cggggggaga caatttgcgc gtaagtcgct
    31621 cgttaacaat caagcagatg gcaatggtgg cagccgttgt cctggtgttc gtttttattt
    31681 tttgcaccgt tttgctgttc catctggtcc agcagaatcg ctataacacg gctacgcaac
    31741 tggaaagcat tgctcgctct gtccgcgaac ccttatcttc agccattttg aaaggcgata
    31801 ttcccgaagc ggaagctatt cttgccagca ttaaaccggc aggcgtggtc agccgtgccg
    31861 atgtagtgct gcctaaccag ttccaggcgc tgcgtaaaag ttttattcca gagcgcccgg
    31921 tgccggtaat ggttactcgc ctgtttgagc taccggttca aatctcgctg ggcgtctact
    31981 cgctcgaacg tccggcaaat ccgcagccaa ttgcctatct ggtgctacag gcggattcct
    32041 tccgtatgta taagttcgtg atgagcaccc tctcaacgtt agtgaccatt tacttacttt
    32101 tgtcgttaat attgacggtg gcgattagct ggtgcattaa ccgcctgatt ttgcatccgt
    32161 tacgcaatat tgctcgcgaa cttaacgcca tcccagccca ggagcttgtt ggtcaccaac
    32221 tggcattacc gcgtctgcat caggacgatg aaatcggtat gttggtgcgc agttacaacc
    32281 tcaaccagca attgctgcag cgccattatg aagaacagaa cgaaaatgcg atgcgcttcc
    32341 cggtgtcgga tttgccgaac aaagccttgc tgatggagat gctggagcag gttgtcgcgc
    32401 gtaaacaaac caccgcgctg atgatcatca cctgtgaaac tctgcgtgat actgcgggcg
    32461 tgctgaaaga ggcgcaacga gaaattctgc tgctgacgct ggtggaaaaa ctcaaatcgg
    32521 tactgtcgcc acgtatgatc ctcgcgcaga ttagcggtta tgactttgct gtcattgcca
    32581 acggtgtaca ggaaccgtgg cacgcaatca ctttgggtca gcaagtgctc actatcatga
    32641 gcgagcgcct gccgattgaa cgtattcaac tccgtccgca ctgtagcatt ggcgtggcga
    32701 tgttctacgg cgatctcacc gccgaacagc tttacagtcg cgctatttct gcggcattta
    32761 ccgctcgcca taaaggcaag aatcagattc agttctttga tccgcagcag atggaagctg
    32821 cccagaagcg gttgacggaa gagagcgata tccttaatgc actggaaaat catcagtttg
    32881 ctatttggtt acagccacag gtcgagatga ccagcggtaa actggtcagt gcggaagtgt
    32941 tactgcgtat ccagcaaccg gatggcagtt gggacctgcc ggatggctta atcgatcgca
    33001 ttgagtgctg tgggctgatg gttaccgtcg gtcactgggt gctggaagag tcctgtcgat
    33061 tgcttgcagc ctggcaagag cgcggcatta tgctgccctt gtcggtaaac ctctctgcgc
    33121 tgcaactgat gcacccgaat atggtggcgg atatgctgga actgttaacc cgctatcgca
    33181 tccagccggg aacactgatt ctggaagtga cagaaagccg acgtattgac gaccctcatg
    33241 ctgcggtggc aatcctccgt ccgctgcgca atgccggagt tcgggtggcg ctggatgatt
    33301 tcggcatggg ctacgcaggg ctgcgtcagc tgcagcatat gaaatcgttg ccaatcgacg
    33361 tactgaaaat cgacaaaatg tttgttgaag gcttgccgga agatagcagc atgattgctg
    33421 caattatcat gctggcgcag agcctgaact tacaaatgat tgccgaaggc gtggagactg
    33481 aagcacaacg cgactggctg gcaaaagcgg gcgttggtat tgcccagggc ttcctttttg
    33541 ctcgtccact ccctattgaa atcttcgaag agagttacct ggaagaaaag tagctacccc
    33601 aaactgatta caaaacttta aaaagtgctg gtttgtgcga gccagctcaa actttttaac
    33661 ctttttgttt caattatgat ccaggtacat ttctgtgatg ttgtctgggt gttattttaa
    33721 ggccgcaggt accccataac cttacaagac ctgtggtttt actaaaggac accctatgaa
    33781 aacctctctc tttaaaagcc tttactttca ggtcctgaca gcgatagcca ttggtattct
    33841 ccttggccat ttctatcctg aaataggcga gcaaatgaaa ccgcttggcg acggcttcgt
    33901 taagctcatt aagatgatca tcgctcctgt catcttttgt accgtcgtaa cgggcattgc
    33961 gggcatggaa agcatgaagg cggtcggtcg taccggcgca gtcgcactgc tttactttga
    34021 aattgtcagt accatcgcgc tgattattgg tcttatcatc gttaacgtcg tgcagcctgg
    34081 tgccggaatg aacgtcgatc cggcaacgct tgatgcgaaa gcggtagcgg tttacgccga
    34141 tcaggcgaaa gaccagggca ttgtcgcctt cattatggat gtcatcccgg cgagcgtcat
    34201 tggcgcattt gccagcggta acattctgca ggtgctgctg tttgccgtac tgtttggttt
    34261 tgcgctccac cgtctgggca gcaaaggcca actgattttt aacgtcatcg aaagtttctc
    34321 gcaggtcatc ttcggcatca tcaatatgat catgcgtctg gcacctattg gtgcgttcgg
    34381 ggcaatggcg tttaccatcg gtaaatacgg cgtcggcaca ctggtgcaac tggggcagct
    34441 gattatctgt ttctacatta cctgtatcct gtttgtggtg ctggtattgg gttcaatcgc
    34501 taaagcgact ggtttcagta tcttcaaatt tatccgctac atccgtgaag aactgctgat
    34561 tgtactgggg acttcatctt ccgagtcggc gctgccgcgt atgctcgaca agatggagaa
    34621 actcggctgc cgtaaatcgg tggtggggct ggtcatcccg acaggctact cgtttaacct
    34681 tgatggcaca tcgatatacc tgacaatggc ggcggtgttt atcgcccagg ccactaacag
    34741 tcagatggat atcgtccacc aaatcacgct gttaatcgtg ttgctgcttt cttctaaagg
    34801 ggcggcaggg gtaacgggta gtggctttat cgtgctggcg gcgacgctct ctgcggtggg
    34861 ccatttgccg gtagcgggtc tggcgctgat cctcggtatc gaccgcttta tgtcagaagc
    34921 tcgtgcgctg actaacctgg tcggtaacgg cgtagcgacc attgtcgttg ctaagtgggt
    34981 gaaagaactg gaccacaaaa aactggacga tgtgctgaat aatcgtgcgc cggatggcaa
    35041 aacgcacgaa ttatcctctt aatctcatca cttatgcccg cactcccttc cccgagtgcg
    35101 ggtgtttgcg cataattgac ccctgtgcct gaacttacca cctgggtgag ttcaggttta
    35161 tttcacttct tccgtgacat tttcatgttc ttgcggtcta acacgaagtg tttttaacgt
    35221 catttagact gaccgacaac gggcagtttt tttattacca ggatagttga tcaggggttc
    35281 acatgcaggg cacaaaaatt cgacttttag cgggcggttt gctgatgatg gccactgctg
    35341 gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg acgctttcca
    35401 acggtttaca gtggcaagtg ctgaccaccc cccagcgtcc cagcgatcgt gttgaaattc
    35461 gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt tacagtcacg
    35521 ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcacag gcgcgttcat
    35581 tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc tcttatgaca
    35641 ccacgctgtt taatctgagt ttgcccaata accgtaacga tttgctgaaa gaggcgctct
    35701 cttatctggc aaatgccact ggcaaactga ctatcacgcc agaaaccatc aaccacgcgc
    35761 tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc tggtggcgtt
    35821 atcgtctgaa aggatcaacc ctgttaggtc acgatcctgc cgatccgctg aaacaacccg
    35881 ttgaagcgga aaaaattaaa gatttctatc agaaatggta caccccggat gcaatgacgc
    35941 tgctggtggt gggaaacgtg gatgcgcgct cggtcgtcga ccaaatcaac aaaacgtttg
    36001 gcgaactgaa aggcaaacgt gaaacaccgg ctccggtgcc gacgctttct ccgctgcgtg
    36061 cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatct atcatgtggg
    36121 atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg cgtgcggacc
    36181 tggcccgtga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt aacagcaaag
    36241 acatcggtct tggatttgac tgccgtgtgc tgtatctgcg tgcgcagtgt gccatcaaca
    36301 tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt gaactggcga
    36361 aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg caaaagaaac
    36421 tggagctgca gaaactgttt gccgcctatg cacgagctga taccgatatt ctgatgggtc
    36481 agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcag tatcagaaac
    36541 tgcggcagga tttccttaac agcctgactg tggagatgtt aaatcaggat ctgcgtcagc
    36601 agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg gaatttaaca
    36661 tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atctaccgcc gctgcgacca
    36721 cctctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct gcacagtaat
    36781 gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg gatagagggg
    36841 taacacttta tccagccttt tgcatatgct gcgtttacgc tggcatcgcc tcacgcggga
    36901 taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt ttcgctgcgt
    36961 tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcactgaaa gagtcgccag
    37021 ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgctggaaca tccactaacc
    37081 cttcgccagc aatagacacc aggcaagaat ccgccccgcg tttcaccacc acttctttca
    37141 cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc cacagcgcgt
    37201 cttcatcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgt tggtacacct
    37261 gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg aaaatcactt
    37321 ttccgccgtt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg ctggtcgggc
    37381 ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc agctctgcac
    37441 aaatcgccgc agactgctcg ctctccagcc agaatttggc agcggcttcg ttacgccagt
    37501 agtagaacgt acgttcaccg gtgctgtcgg tttcaatgta gtaaaggccc ggcagacggt
    37561 tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gtgccaggcg tccagcattt
    37621 gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat gccgcaggat
    37681 cgacctgacg ggcgatatag acggaagtat tcagtgtatc gccgccgaaa ccgcgcttaa
    37741 cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca atctttttgg
    37801 acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg gtggtttgct
    37861 ggtcaaccat attaaaacag tgttccatta tttttttgag ccagaacgta ttcctgaaag
    37921 attccgttgt ggagtgagga aatttacccc ggccaggcgg taaagttctg cccttacgcg
    37981 ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat ctttgatgat
    38041 aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct tgcaggaacg
    38101 gcgtttttgg ttgcagtgtg agcgtgctta cacttggcag ccgatctatc aaacatgcgg
    38161 gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc cttcgcaacg
    38221 cctgccgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg aggttgtgaa
    38281 agagcagatt gatttgctgg cgcaaaaagc cgacttcttt atagagcacg gcctgctggc
    38341 atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa aaatcctgcg
    38401 ccaaattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatcc gtctgccgaa
    38461 agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg attttggtac
    38521 cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca aaatcgcgcg
    38581 agaactgttt gtgatgctgc gtcagtcgcc ggaaggccgc acactctttt ctcagctttt
    38641 acatctaatg aatcgctatt gtcgcggggt gattgtcgag ggcgtagaaa caccggaaga
    38701 gtggcgtgat gttcagaact cgcccgcatt cgccgcacaa ggctggtttc tttcacgccc
    38761 ggcaccgata gaaacgctga atacggcggt tctggcgcta taagctgcct cattttccgc
    38821 ctggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa cgatgagcaa
    38881 ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg tcgtggcgat
    38941 cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc agaaagtctc
    39001 tgcggagttg aatcgtccgt tcgctatccg tggcgatctg ggcgtggtgt gggagcggca
    39061 aaaacaagaa actggctggc gcagctgggt gccgtggccc catgtacatg cggaagacat
    39121 cattcttggc aatccaccgg atattcccga agtcacgatg gtgcatttgc cacgcgtaga
    39181 ggcaacgctg gcaccgttgg cgctgctgac caaaacggtc tggctaccgt ggatcaaact
    39241 ggaaaagccc gacgcacgcc tgattcgcct ctctgaaaag aacaataact ggacgtttaa
    39301 tcttgccaac gatgacaaca aagacgcgaa tgcaaagccg tcggcatggt cgtttcggct
    39361 ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa gcaaagcgga
    39421 tctggaaatt tttgtcgatc cgttaggcaa gccactgccg ttcagcgaag ttactggatc
    39481 gaaaggtaaa gcggataaag aaaaggtggg cgattacgtt tttggcctga aggcgcaggg
    39541 acgttataac ggtgaaccgc tcactggtac gggaaaaata ggcggtatgc tggcgctgcg
    39601 tggcgaaggg acgccgtttc cggtacaggc tgatttccgt tcaggtaata cccgtgttgc
    39661 ttttgatggc gtcgtgaatg acccaatgaa gatgggcggt gtcgatttac ggcttaaatt
    39721 ttctggcgat tcactgggtg atctctatga actgacgggc gttctgctgc ccgatacccc
    39781 gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat cgtcggtctt
    39841 tgattatcgc ggttttaatg ggcgcattgg cgatagcgat atccacggtt ctctggtcta
    39901 caccaccgga aagccacgac caaaactgga aggtgatgtc gagtcgcggc aattgcggct
    39961 ggcggacctg ggaccgttga ttggcgttga ttccgggaaa ggggctgaaa agtcgaaacg
    40021 gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggtaaagtgc tgccttatga
    40081 ccgcttcgaa accgataaat gggacgtcat ggatgccgat gttcgtttca aaggccgacg
    40141 tatcgagcat ggcagtagcc tgccgattag cgatctttct actcatatca tcctcaaaaa
    40201 tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggtggcagca ttgcggcgaa
    40261 tattcatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc aggctcgtcg
    40321 actgaaactg aaagaactga tgcccgatgt ggaactgatg cagaagacgc tgggggaaat
    40381 gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt taggcaacag
    40441 taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc tgatggagat
    40501 tgttgggctg aatgtcggca actacattgt cggtgcgata tttggtgacg atgaggtgcg
    40561 ggtgaactgc gcggcggcga atctgaatat tgccaacggc gtggcacgcc cgcagatttt
    40621 tgctttcgat actgagaacg cgttgattaa cgttaccggc acggcaagtt ttgcttcgga
    40681 acagctggat ttgactattg atccggagag taaagggatt cggattatca cactgcgttc
    40741 gccgctgtat gtgcgtggga cgtttaaaaa tcctcaggct ggggtgaaag ccgggccgtt
    40801 gattgcccgt ggtgctgttg ctgcggcact ggcaacgctg gtaacaccgg cggcggcgtt
    40861 actggcgctg atctcacctt ccgaagggga ggctaatcag tgtcggacga ttttgtcgca
    40921 gatgaagaag tgatgataat tgccggatgt ctgacatccg gcaattaaac agatttagat
    40981 tgttggcaaa gtgtgctttg ttcatgccgg atgcggcgtt aacgccttat ccggcctaca
    41041 aatccttgtg aattcaatat attgtatgga aattcaggcc tgataagcat agcgcatcag
    41101 gtttttcact cttattacaa cgactgatgt cgcgtctcat gggtcagcag cagggcgatt
    41161 aacgtcaagc cagccatcgc cgccagatat aaccccaccg cacctagccc gtagttagtc
    41221 tgcaaccagg ctgcgatata tggcgcaacg gaagccccga gaatcgacgc tacgttgtaa
    41281 gagaacgatg ctccggtgta acgcacttct gtcggaaaca gctctggtaa cagcgcaccc
    41341 attggcccga aggtcagccc catcagactt aaccccagca gcaggaaggc aaaaaccaga
    41401 atcgggttgc cagaaccgag cagcgggtta aaggcgaaca gcgcgaacag gatgatcagc
    41461 gtggtgataa ttaccatgct tttacggcga ccaaaggcat cagccagtaa tccggcgact
    41521 ggcaccatca cgccaaaacc aataactgcc atcatcagca tccacaacac ttcgttgcgc
    41581 ggcaggccaa gcccaactgg cgcggcggcg gtactaaagg tcatagagta gaccgtcatg
    41641 atgtaaaata gcgtataggt tgccagcata atgaacgtac ccagtacggt tacgcgaaca
    41701 tgtttggtca gcagcgtacc cagcgggatc ttcacctgtt ttttcgcttt agcgactttc
    41761 tcaaacaccg gcgactcatg cagcgacacg cgaacataca ggccgataat gaccagcacc
    41821 gccgagaaga taaatggcac acgccagccc cagctcataa actgctcgtc ggtcagtagc
    41881 caggaaagca gcaagaaagt gccgttggca aagaagaagc caatcggtgc gcccagctgt
    41941 gggaaggagc catacagagc acgtttgcgc ggtggggcgt tttcagtcgc cagcagcgcc
    42001 gcaccgcccc attcaccgcc taagccaaga ccctgaccaa atcgagccag cgccagcagc
    42061 agcggggcga aaataccaat cgtggcatag cccggcagca gaccaatcac cacggtcgaa
    42121 atccccatcg ttagcaacga ggcgaccagc gtcgctttac gcccaacgcg atcgccaaaa
    42181 tgaccaaata cggcagagcc aatggggcgc gcgacgaagg cgatggcgaa ggtggcgagc
    42241 gactgtagcg ttgctgccgt tggatcgccc tgcgggaaga agatatgcgg aaacacaata
    42301 acggccgcag tggcgtaaat gtaaaagtcg aagaactcaa tggccgtgcc aatgagagag
    42361 gcgacaagga ctttattacg cgagttgatc ggcgtgtatt cttgctcgtg gtcgagtgtt
    42421 gtggctgttg cttgcataat cttttcttat tttttggcta acgaatagcc atattacgca
    42481 cagcaaaagt gatatttcaa tgtgtaacaa atcgcggaaa tggtcaaaaa taaggcaaaa
    42541 aggggataat gtttaggcca gtgatttttc ttctatgaaa tgcttcacat aattttataa
    42601 atggtggata aaactggttt caggttaaat aaaagttacc aactggattt atatgctggg
    42661 gttatgagtt ggcatgaaat tgtttgctcc caaatagatg gctgaagagg ctctggggaa
    42721 gagtttcaag aggttatgtg cgcattatag ccggatgcga tgctggcgca tcttatccgg
    42781 cctacgggga gccaggccga ataaattcgt acgccgtatc cggcctattt taaggctgtg
    42841 ttttccccgg cattcgcggg tcgtctttat attcggcggt ggcaatccac gccgcgcaaa
    42901 acagcgtcaa acgggcgaag aagtagaaaa atgccatcag ccccagcacg gaaccaaatg
    42961 ctgcgccaga gggggatttc atcagcgatg gtagggtgta ggtcatcacg attttaatca
    43021 cttcaaaacc aatagcagcc agaaatgttc cgcgaatcag ggcttttttg cgcggacggt
    43081 ggcgcggtaa ccgccagaag atccagaaga aaagcagata gttggcgaag atggaaatcg
    43141 ccaggccaat caaccgccac gtcggtttca gccactcaat gctgttcagg tgcagggcgc
    43201 taataatcat ttgctgcgcc gaaccggcaa ccgaggtgat cgaaagcgtc acaatcagcg
    43261 caatcaacaa accaatcagc gaaataaaat cacgcagata ttttacccag aacttctcct
    43321 gatcttgcgg cgagcgttcc cagacatcgc gcgactgggc gcgaatcgct tcacgcaggt
    43381 tacccatcca gttgatgccg gaataaagcg ccaccgccag gccgacaagc cctacagtcg
    43441 tacgctgctg aacggcggtg ttgatggtgt ttttcaacgt agcggctagc gtcggatcgc
    43501 tgatgttttg cagaattttg tcgaagatat cctgtagcaa catcggatgg gaggccagca
    43561 caaagccccc tgcggcaaac gacaccatca aaatcgggat catcgacaaa aatgagaaat
    43621 aggtgatagc cgcaccaaac tggttaccca gccgatcgtt aaagcgttct gtcgcgcgaa
    43681 taaggtgcgc aataatcggt tggcgttgga ctttttcggc agtggtggtg acggtttcca
    43741 gcgcctggct aactttgccg cctttctcgc tattatccaa cggcttaatc ggctcgtgct
    43801 ccagatcctg ggtgggacgt ttgatctcgt tttcctgcgt catccgctct tttgtccttc
    43861 tgtcgtggtc attcttaaaa agtatagtcg gtcagtccag gtactctttc attacgccag
    43921 ccagccactg cataaacagg tttacacgcc gggaaagctc ccgacgctgc ggataaacca
    43981 gcgaaaggga gagcggctcg gcacggtagc ctggcaacac ttcaataagc cgcccggcac
    44041 gcagggcttc gcgcacggca atgcgcggga tctgaataat ccccagaccg gcaagaccgg
    44101 cggcgagata gttttcgcta ctgtttaccg tcaacatgcc gccagactta aaccactgga
    44161 cgccattaac gctggcaacc tcaaaaccta acggatgtac acccaggtgc ggtgtataac
    44221 gcactattgc gtgtgaagtc agatcatcgg ggctttgcgg ataaccaaag cgcgtcagat
    44281 agtgcggact ggcacagttg accatggtca gtttgccgag gggacgggcg ataacgccat
    44341 cttccggtaa cgcgccagtg cgtatcacgc aatcaaaacc atcatgaaga atatctaccg
    44401 gacggtcatg gctactcagt tccagctcaa ttcccggatg cagatagaga aattccgaca
    44461 ggcgcggcag taacaggctt ttcgcgattc ctggcgggat gtcgatgcgt aatttaccgc
    44521 tgatactggt ggcatcctgt tgaaacagac cgtccagttc gctgagatta ctcaacacat
    44581 cttttgctcg ttgataatag gtcatgcctt ctggcgtgag tttgacccgt cgcgtggtgc
    44641 gctggagaag ctgggtgcca agttgatgtt ccagtgcctg tatctggcgc gaaacacttc
    44701 cttttggcaa agcaaagaaa tccgccgcgc gggaaaaact ttccagctcc gcgactttga
    44761 tgaacaactg cattgcgtga attttatcca tttctttgac cgattgttgt tcacagcgaa
    44821 acagtaacgc gcatatcgca ttgtttattg ttttttctaa catctaatgc cgggtttcac
    44881 aatgttgtca tgacgaatgt ggcgatttct cagcaggaaa agggcggaat agctttctga
    44941 gaaaggtgaa tccccttaaa cagagcgtat ttttcagcga ttccctttaa agtgaataga
    45001 ggacgtattg gctatttgtg cttatttttg tctcattgcc atttgttatt ttggcttgtg
    45061 gtcgcggaat ttcattttta attgcatgcg tactaaccat taatataagg aaatcctcaa
    45121 aattgtttta aatagtgccc tcgacatgta atttcaggcg tttagctgta actatttata
    45181 tcggtaacta ttttctacgc gccatctctg tagaatggcc accgccaaat ggcgaattct
    45241 tattttttct cgcgggtaat acccttccac tacttaagtc tttttaacgt attcaggtgg
    45301 agattcagtt atgcaaatag tcatgtttga caggcagtca atatttattc atggaatgaa
    45361 aatcagttta cagcagcgta ttccaggagt gagtattcag ggggccagtc aggcagacga
    45421 gctatggcaa aagctggaaa gttaccctga agccttagtt atgctcgatg gcgatcagga
    45481 tggtgagttt tgctactggt tgctgcaaaa aaccgtggtg caatttcctg aggttaaggt
    45541 gttaattacg gcgacggatt gcaataaacg gtggttacag gaagttattc attttaatgt
    45601 gctggccatt gtgcctcgtg attcaaccgt cgagacattt gcgttggcgg taaatagtgc
    45661 ggcgatgggg atgatgtttc tgcctggaga ctggcggacg acgccggaaa aggacattaa
    45721 agatctcaaa tcgttaagcg cccgccagcg ggagatttta accatgttag cggcgggcga
    45781 atcaaataag gagatcggca gggcgctgaa tatcagtacc ggaacggtaa aagcgcatct
    45841 ggaatccctg tatcgccgtc tggaagtgaa aaatcgcacc caggcggcaa tgatgttaaa
    45901 tatctcctcc tgaagagaat aattttacag ccagcaggaa gttagcggct ggctgtaaaa
    45961 atattatggt tcgccgtaca aaccaattaa acggcgtacc acaccgttag tccagccaaa
    46021 cccatcctgc aacggatact cgccaccgcc gccttcgcgg ggaacaccat cggcaatatg
    46081 gtatttctcg atcagtttgt gctgttccag atagaactga ttcaccgtct tcagccagct
    46141 gcgcgcgatt tcatcaccca gaaggtcatc tccatacatt ttaaatccct gaatcgccat
    46201 ccattgtaac ggtgcccagc cgttgggttt atcccactgt tcaccggttt cgtactcgct
    46261 tgccagaatc ccgccaggtg tcagtaaccg actgcgcacg gcgtttgcca gacgatcggc
    46321 ctgttcatgg ttcgccatac cgacatagag tggcacaatg gcggcagcgg aaaacagcgc
    46381 cagttgttcg cgtcgccagt cgtaatcgcg gtagatgccg ttttcatcat cccagaggta
    46441 acggtttacc gcatcgcgac gggcactggc cttctggcgg aacagtgctt ctgtctcttt
    46501 ctcacctttc agcgccgaga tgttggcgat ggcgctctcc agtttaaaca ggaaggcatt
    46561 cagatcgatg gggatgaact gggtggtacg aatgctcgcc agacgaccag tatcacgcag
    46621 ccaacgggaa gagtaatccc aaccggaggc cgcccccgcg cgtaaatcgc ggtacacctc
    46681 gttgggcggg cgaccagaat gtttcgcggt ttcaacgtcc tcaagccagg attcgtcacg
    46741 cggcgtgtcg cgatcgtccc agtaacggtt gagcagcgat ccgtccggca tccgcacaac
    46801 atggcgatag gcctgattag ggatcaacga ttctgcaccg tccatccaga aggcatattc
    46861 cattttcagg tggtcgagat agcggcgtgc accgcgtaca ccatcttctt caaacaactc
    46921 caccatcagc gcaaaaaccg gtggttgcga gcggctcaaa taataggtgc ggttgccgtt
    46981 ggggatgtga ccgtagtttt cgatcatcca ggcgaagtta tcggccatgc atttcagcaa
    47041 atcttcccga ccactttccg ccagccccag catggtgaaa taggaatccc aatagtaggt
    47101 ttcgctaaaa cggccgcccg ggacaatata tgactgcggc agcgccagca gagaagacca
    47161 cggaatgtga tcctgtggtt cgcgggttag caccggccac agctggtcga tatgctcttt
    47221 cagggaattt tgcgggtccg atacatactc gctggagtag acctccggca gccagaagtg
    47281 gttttcaaca aacttgcgca agtcaaaatc acgatgacgg cgcactttac ggtagcggat
    47341 taaaatatcc agcgggtcca ttttaggtgc gcagtcggga aaggttttac tgtcggggaa
    47401 aattttcgcc gactgaacat gctcgaacag ttcgagatag cgatcggcag gcgtcagcgc
    47461 atcagaggcg ggcagccctt caatcatctc gggttccggt tctgcctcga tcatctcatc
    47521 cagttttaat tcatacgggt ccagttcata gcagagatcg atttcgatca tcagttcgtc
    47581 tggattaggg ttttgaattt tctgattgag cataaggatc caacctccga aattcgtcgc
    47641 taattatcgg gcttttcttt aagggtagtc attcgctccg cctggtgggc gacaaagcgt
    47701 gcggtggatc acgtttatta acctgtcaaa aaatataacg cactaatatc catggaattt
    47761 ataaaaatac agatttggtg ttgtttttca ttttcagaac attcaatcct gctctcgccg
    47821 caatcatgat atgccattcc tgatctgggt aataggcaca ggctatctta ttgatagttt
    47881 atattcatgt aattgattga ttcctatcaa aaagtgtatc tccctgtttt ttatgatggc
    47941 tttgtctcgc agagacgttt gcagcattct ggaaaatttt ctgggtcaca ataactcacc
    48001 accgtgaggg aagaataatg aaaatggtct cacgtattac cgcgatcggc ctggctggcg
    48061 tcgcgatttg ctatttaggg ttatctggtt atgtgtggta ccacgataat aaacgcagta
    48121 aacaggccga tgttcaggca tctgctgtca gtgaaaataa taaggtttta ggctttctcc
    48181 gcgaaaaagg atgcgactat tgccacacgc cttcggcaga attacccgcc tattattata
    48241 ttcctggcgc gaaacagttg atggattacg acattaagct tggatataaa tcttttaacc
    48301 tcgaggccgt gcgtgcggca ctgctggctg ataaacccgt ttcgcaaagc gatctgaata
    48361 agattgaatg ggtgatgcag tatgaaacta tgccaccaac gcgttatacc gcgctacact
    48421 gggcgggtaa ggtgagtgat gaagagcggg cggaaatact ggcctggatt gcaaaacagc
    48481 gcgcggaata ttacgccagc aatgatactg ctccggagca tcgcaatgaa ccggtgcagc
    48541 ccatcccgca aaaactgcct accgatgcgc aaaaagtggc gttgggcttt gcgctgtatc
    48601 acgatccccg tttatcggct gatagcacca tttcatgcgc tcattgccat gcgttgaatg
    48661 cggggggcgt cgatggcaga aaaacatcga ttggtgttgg tggcgcagtt gggccgatta
    48721 acgcgccgac ggtatttaac tcagtattta acgttgagca gttctgggat ggtcgtgcgg
    48781 caacattgca ggatcaggct ggtggaccgc cgttgaaccc gattgaaatg gcgtcgaaat
    48841 cctgggacga aattattgct aagctggaaa aagatccgca gcttaaagcg cagttcctcg
    48901 aagtctatcc gcaaggtttc agtggcgaaa atattactga tgccattgct gaatttgaga
    48961 aaacattaat tacgccggat tccccatttg ataaatggtt gcgtggagat gagaatgcgc
    49021 tgacggcgca acagaaaaaa ggctatcaat tatttaaaga taataaatgt gcaacttgtc
    49081 atggtggaat tattctcggc ggacgttcct tcgaaccgtt ggggctgaaa aaagacttta
    49141 actttgggga aattacggcg gcggatattg gtcgtatgaa tgtgactaaa gaagagcgtg
    49201 ataaattgcg tcagaaagta cccggtttac gtaacgttgc tttaacggca ccgtacttcc
    49261 atcgcggtga cgtgccgacg ctggacgggg cggtgaaact gatgctgcgc tatcaggtag
    49321 gcaaagagct gccgcaggag gatgtggatg atatcgtagc tttcctgcac agtctgaacg
    49381 gggtttacac gccgtatatg caggataaac aataattaat ttgatcgccc gaacaggaat
    49441 gtttgggcga tttttattac gataataaag tctgttttta atattatcat gttaaatgtt
    49501 tatattataa aagatcattt ttctgcttag gattttgtta tttaaattaa gcctgtaatg
    49561 ccttgcttcc attgcggata aatcctactt ttttattgcc ttcaaataaa tttaaggagt
    49621 tcgaaatgga ccagaagctg ttaacggatt tccgctcaga actactcgat tcacgttttg
    49681 gcgcaaaggc catttctact atcgcggagt caaaacgatt tccgctgcac gaaatgcgcg
    49741 atgatgtcgc attccagatt atcaatgatg aattatatct tgatggcaac gctcgtcaga
    49801 acctggccac tttctgccag acctgggacg acgaaaacgt ccataaattg atggatttgt
    49861 cgatcaataa aaactggatc gacaaagaag aatatccgca atccgcagcc atcgacctgc
    49921 gttgcgtaaa catggttgcc gatctgtggc atgcgcctgc gccgaaaaat ggtcaggccg
    49981 ttggcaccaa caccattggt tcttccgagg cctgtatgct cggcgggatg gcgatgaaat
    50041 ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac ggataaacca aacctggtgt
    50101 gcggtccggt acaaatctgc tggcataaat tcgcccgcta ctgggatgtg gagctgcgtg
    50161 agatccctat gcgccccggt cagttgttta tggatccgaa acgcatgatt gaagcctgcg
    50221 acgaaaatac catcggcgtg gtgccgactt tcggcgtgac ctacaccggt aactatgagt
    50281 tcccgcaacc gctgcacgat gcgctggata aattccaggc cgacaccggt atcgacatcg
    50341 acatgcacat cgacgccgcc agcggtggct tcctggcacc gttcgtcgcc ccggatatcg
    50401 tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc ttcaggccat aaattcggtc
    50461 tggctccgct gggctgcggc tgggttatct ggcgtgatga agaagcgctg ccgcaggaac
    50521 tggtgttcaa cgttgactac ctcggtggtc agattggtac tttcgccatc aacttctccc
    50581 gcccggcggg tcaggtaatt gcacagtact atgaattcct gcgcctcggt cgtgaaggct
    50641 ataccaaagt acagaacgcc tcttaccagg ttgccgctta tctggcggat gaaatcgcca
    50701 aactggggcc gtatgagttc atctgtaccg gtcgcccgga cgaaggcatt ccggcggttt
    50761 gcttcaaact caaagatggt gaagatccgg gatacaccct gtacgacctc tctgaacgtc
    50821 tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc accgacatcg
    50881 tggtgatgcg cattatgtgt cgtcgtggct tcgaaatgga ctttgctgaa ctgttgctgg
    50941 aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag ggtattgccc
    51001 agcagaacag ctttaaacac acctgataac ataacgttgt aaaaaccgaa tgcccagcct
    51061 ttaaaaaaac agctgggcat tcggttgctt attaatcgca ataatatatt ggctgtctat
    51121 tcatcgtgtt aataagatat atgctatatg aatgtttatt aaatgtagcg attaccttta
    51181 atgtcacgaa aagatattat ttgcttacta ttaatttccc tgcgttctta aacacgtttt
    51241 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
    51301 cgcttcgttt aaatttttta tcaatcaatt tgacttaaga gggcggcgtg ctacattaat
    51361 aaacagtaat atgtttatgt aatattaagt caactatgca atcactacat gggaattgtt
    51421 taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa tatcgctatt
    51481 ttaatggcgg tgacctggtt tttgcggatg caagccaaat tcgagtagat aagtgtgttg
    51541 aaaattttgt attagtatca agggatacgc tttcattatt tctcccgatg ctcaaggagg
    51601 aggcattaaa tcttcatgca cataaaaaag tttcttcatt actcgttcat cactgtagca
    51661 gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat cttcgctatg
    51721 cagaaatgct acgtaaaaga gcattaatct ttgcgttgtt atctgttttt cttgaggatg
    51781 agcactttat accgctgctt ctgaacgttt tacaaccgaa catgcgaaca cgagtttgta
    51841 cggttatcaa taataatatc gcccatgagt ggacactagc ccgaatcgcc agcgagctgt
    51901 tgatgagtcc aagtctgtta aagaaaaaat tgcgcgaaga agagacatca tattcacagt
    51961 tgcttactga gtgtagaatg aaacgtgctt tgcaacttat tgttatacat ggtttttcaa
    52021 ttaagcgagt tgcagtatcc tgtggatatc acagcgtgtc gtatttcatt tacgtctttc
    52081 gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag agattgccga
    52141 accgtgactc ggcggcaagt attgttgcgc aagggaattt ttacggcgct gactgttctg
    52201 cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc ataggggacc
    52261 gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc cctgttggca
    52321 cgggaaactt tgtgctctca gtaagttaaa tataactttt actggaaata agatcagcca
    52381 tttttttata aacataagct atacgctgtg cgaaaatata ttcgtgctgc atttacttat
    52441 tatcaattaa ctgttatgca aaactacttt gtggataaat tttggtccta ccaaatctgg
    52501 cagtttttgc gctaagaaac agtctggcat catttcatta gtatactgaa attgaaataa
    52561 tcgcagtatg aaatataagg gataatcatg actcatgtct gctcggtgat cctcattcgt
    52621 cgttcattcg atatttatca tgaacagcaa aaaatatcgc tgcataacga gagtatcctg
    52681 ctgctggaga aaaatttggc agacgatttt gcgttttgtt caccggatac gcgacggctg
    52741 gatatcgatg agctgacagt ttgccattac ttacaaaata ttcgtcagct accacgcaat
    52801 ttagggttac atagcaaaga ccgtttgtta attaaccagt caccccccat gccgctggtg
    52861 acggcgattt ttgatagttt caatgactcc ggggtaaatt cgccgatact gagcaaaatg
    52921 ctctatcttt cctgtttatc gatgttttct cataagaaag aactgatccc cttacttttc
    52981 aatagtatca gtactgtttc aggaaaagtt gaacgcctta ttagctttga tatcgccaaa
    53041 cgttggtatc tgcgcgatat cgcggaaaga atgtatacca gcgagagtct aatcaaaaaa
    53101 aagttgcagg atgaaaatac ctgtttcagt aaaatattac tcgcttccag gatgtcgatg
    53161 gccagacgat tactcgagtt acgtcaaatt cctctgcata ctattgcgga aaaatgtggc
    53221 tatagcagta cgtcgtactt tataaacaca tttcgacaat attatggtgt aacgccacat
    53281 cagtttgcgc aacattcgcc aggtaccttt tcctgacata ttttgcattt gaatattggt
    53341 caggatctca cacctgcttc atgtgaaact cttccctgat gatttctgcc gggctaccga
    53401 ctagttctct ttcgcagttt gccaggcggt ataggtattt tcaggaggaa aagactcggc
    53461 atgtttggga ttattaagct gacaattcat acgattacgg gaatgtgggt cagtattgtg
    53521 ctgttcaaat tgatgacgaa tggctggagt ggtttctact ttcagtgctg tgtactaagc
    53581 ctggtattcc tgacggttag ctggttgttg agtggtgaat ggttagcagg aaagagtaag
    53641 gctgaacctt cacgttcaac cttactctca tttacacgtt acgctttttt aaagcgggca
    53701 aagagatgtt ccactacaac gaaaaagacc ggaacaaagt aaattgccag cactgttgcg
    53761 gcaaacatcc cgcccatcac gccggtacct accgcgtttt gcgcgccaga accggcacca
    53821 tgactgataa ccagcggcag cacgccaaga ataaaggcca gagaggtcat caggattggg
    53881 cgtaaacgca ttcgtgccgc ttcgatgatt gcctctatcg gcgttttccc ttctttctgc
    53941 atcatctcaa cggcaaattc gacaatcagg atggcgttct tggcggaaag cccgatggtg
    54001 gtcagcaaac caacctggaa gtagacgtca ttacttaagc cgcgcagatc ggtggccagt
    54061 aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg agaacggaat tgaccagctc
    54121 tcataaagtg cggcgagggc gaggaacacc acgaccagtg aaatggcata tagcgcggga
    54181 gcctgatttg aggataacgc ttcctgatac gacagtccgg tccatgagta gccgacgcct
    54241 gccggaagtt tagcgaccag gtctgccata aatttcattg cgtcaccggt acttttcccg
    54301 gccgccgctt cacctaaaat ctccattgac gggatgccgt tgtagcgttc cagtcgcggt
    54361 gaaccatagg tccattcggt agacgagtag gcagaaagcg gtgccatcgt gccagaggcg
    54421 ttgcgtacat accattggtt gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg
    54481 acatacactt ttttcacccg cccctggttg aggaagtcgt tcacgtagct gctgccgaag
    54541 gcggtggaaa ttgtctggtt gatatcagac agcgccacac ccattgcctc agctttcgcg
    54601 gcgttgacgt tcactttgaa catcggcgta tcttccaggc cgttcgggcg taccccggtg
    54661 acctgattcg gtgattgcgc tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt
    54721 tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa aacctgacgc ggtacccagt
    54781 tcagccaccg cgggtaagtt gaacgggaag acgacggctt tattgatact gcttagcgca
    54841 atcattgccc gctgaatgat cgcggtaacc gagttttcct caccgacacg ttcagaccac
    54901 ggcttgagac tgataaacgc caggccgttg ttttgcccct ggccgctgaa gccaaagccg
    54961 ccaacggtaa acaccgactg gacattatct ttctctttag tcagataata atccgtcacc
    55021 tgttgcagca ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc
    55081 ataaataccc cctgatcctc ttctggtaag aaagaggtcg gcgtgcgcag gaacagcacc
    55141 gccatcccgg cgcaaatcag gaggtagacc accatgtagc gaccggtaca acgcaacagt
    55201 gagcgggtgc tatcggtata gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg
    55261 aacagggcgt taggtttgtg accgccttcc ggcgcggctt tcagaatggt agcgcacagg
    55321 gcaggggtca ggctcattgc cacaaatact gaaagcagca tggaggagat cagcgtgatg
    55381 gaaaactggc ggtagatctc cccggttgca ccgctcataa aggccatcgg cataaacact
    55441 gcggaaagaa caacggcaat accgaccagc gcacgttgga tctgccccat cgatttatgc
    55501 gtcgcttcct tcggcggtag cttatcttcc gcaatgacac gctcgacgtt ctccaccacc
    55561 acgatggcgt catccaccag taaccctatc gccagcacca tcccgaacat agtcaaagtg
    55621 ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac caccggtacg
    55681 gcaatcgtcg ggatgattgt ggcacggaaa ttctgcaaaa acagatacat gaccaggaag
    55741 acgaggatga tagcctcaac cagtgttttg aaaacttcct gaatagaaat ttcgataaac
    55801 ggcgtggtgt cgtaaggata aaccgtcttc agacttgccg ggaaataggc tgataagcgg
    55861 ttcagttcct ctttgactgc ccgcgaggta tccagggcgt ttgctccggt agccagtttg
    55921 atggcgatcc cggcagcagg tttgccgtta tagcgcgcca cggtggaata atcgtccgcc
    55981 ccaagttcga cgcgggcgac atcacgcagc agcacttgcg aaccatcttg ctgaactttc
    56041 aacaggattt tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg
    56101 tttagttgct ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga aatctggttg
    56161 ttttgcacct taatctggga aataacatcg gaaggtacca gattgtattt attgagtttt
    56221 tgcgggtcca gccagatacg catggcgtac tcggaaccaa agagctgtac gctaccaacg
    56281 cccgcggtac ggcttagcgg gtctttgata ttagacgcta catagtccgc gatatcgtac
    56341 tggtttaggc tgccgttatc agaaataaac gccgctacca tcaggatatt actgctcgat
    56401 ttatcgacgc taatcccctg ctgctgcact gcttcaggta atgaaggcat agcgagttgc
    56461 agtttatttt gcacctgaac ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga
    56521 gtgatagagg cattacccgc cgcatcactg gttgaagaca tgtacatcag gccatcaagc
    56581 ccattcatat tttgctcaat cacctgagtg accgagtctt ctaccgtttg cgcatcggca
    56641 cccggatagg tagcgctgac ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc
    56701 ggtaagttca tgatcgccag accacctgca agcatcataa taatggcaag tacccaggca
    56761 aaaaccgggc gatcaataaa atagttagcc atgaaagtcc ccttaagcct gcaacgttat
    56821 tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc
    56881 ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg
    56941 acaacccact ggtcgccgat ggctttgctg gcttcaattt cgcgtagctg cacgacatcg
    57001 tctttatcca gaatgagcgc cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc
    57061 ggcaccagta atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca
    57121 ggcagcaagt caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt
    57181 tcatcaaccg tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt
    57241 tccagattga gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc
    57301 tcttctttca tgcgtaagaa atcctgcacc gactgcgtga gatcgacata aatcgggtcc
    57361 agacgttgta cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc
    57421 accgacgatt tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg
    57481 atcgtcgcct gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcaac
    57541 tgggtgcgcg cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc
    57601 tggcggttaa aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct
    57661 ttggcggagt ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc
    57721 tggttcactt tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga
    57781 cgtatctcgg caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc
    57841 gacccagggg agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc
    57901 gatttgtcat cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt
    57961 cttcttctgt tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg
    58021 cgcgaggaga gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa
    58081 ggtgggatac aggcatagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt
    58141 tctgccaaca gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata
    58201 ccaggggaat aaagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag
    58261 catctgcatc cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat
    58321 aagatgtgat acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa
    58381 tgttgaattt ctgaatcttt aatgtttgtt gcgaaatatt aagcgccgac gtgatattgc
    58441 tttcattttt atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata
    58501 attgtgaatt cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac
    58561 tttctttgcg gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag
    58621 gtgccaaaac aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat
    58681 caaggatatg attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt
    58741 tgatctctga cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc
    58801 cctgtaaaag aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta
    58861 tcgtttcgaa catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata
    58921 tagtaaacgc ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca
    58981 atacttcaga tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg
    59041 ttatcagctt gtaaatagca aacaaaaagt taacgtttgc ggattactgc ccaagaataa
    59101 gttatcaata accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga
    59161 tttaaatatc gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt
    59221 taacgcattg aaattacctg agtaaataac acattcatgt cttcaggttt ttatatagcg
    59281 tctggagaca cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg
    59341 attacttatt taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac
    59401 agcactttta aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat
    59461 ggctaatttg ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact
    59521 agcaggtaat tatttcaatg atagtgcgca attgatctac aactccgcgt agcggagaga
    59581 gtattaatcg gatcatagtc acatcaagtg actatgatcc gggtgacaac cgggataatt
    59641 attgctgctt aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt
    59701 caattcccac cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata
    59761 tccaggcaat gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg
    59821 aacgctgacg ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa
    59881 taaatgccgc aatggcaaaa atgcccagct ccggtgcgcg gatgaagaaa tagccgatca
    59941 ataaataggc gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt
    60001 tgctgaataa cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg
    60061 tgcttaaaat atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca
    60121 gcagcacggc aataaactgg attgctctgc gatgtttttt aagcatctcc agatcaaact
    60181 tcaaaattgt tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa
    60241 tcccctgcta tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat
    60301 ttcctaattg cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat
    60361 tttgatattt tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa
    60421 attgattcgt gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta
    60481 ggcgttattc ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa
    60541 aaagcagctg ataacaaaaa accggtcaac tcctggacct gtgaagattt cctggctgtg
    60601 gacgaatcct tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa
    60661 ccagaagatg cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag
    60721 gcttgtactc aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa
    60781 attaagaaag atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca
    60841 ggccggaata tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg
    60901 aatgggttac aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt
    60961 agcagctttg tcactggtga acgcacaatc tgcgctggca gccaatgaat ccgctaaaga
    61021 tatgacctgc caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg
    61081 gatgctgcat gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga
    61141 tctcactcaa attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta
    61201 taccttcaaa aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga
    61261 gtagcaagtt gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat
    61321 tatacgtctt atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatacg
    61381 cggcaaagga gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat
    61441 gattgtttcg aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactggaccc
    61501 cagccgtatt gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct
    61561 cgttcgtaac caaaatattg taggtctgac gactgcagcg gatatctggg tgaccgccgc
    61621 cattggtatg gttattggca gtggtatgta cgaactgggt atttatggtt cagtgatgac
    61681 cttgctggtg ctggaagtct tccatcaatt aaccttccgc ctgatgaaca aaaattacca
    61741 tttacagctg acactagtga acggtaatac ggtatcaatg ctcgactggt ttaagcagca
    61801 aaaaataaaa actgatttag tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat
    61861 tgatatccag cttcatgcca caacgtcgat agaagatctg ttgcgattat taaaaggtat
    61921 ggcaggggtg aaaggcgttt caattagcta atctgtatag ttatttattc gcaggagtac
    61981 cagtgcggta ctcctgttgc ttttaatgat cgaggacgat cagtggtgaa tggttggcag
    62041 gatctttaag tacggtaata tccgcgccgt agagcattgt cagcgcctgc tgtgtcaata
    62101 cgtcctgtgg tttaccattt gcgataacct ttcctttttg cattaataca acacggtctg
    62161 cataacgcgc ggcaagattt agatcgtgca atatgcagca gacattgaac tgccgctcgt
    62221 gaactaactg gcgtaataaa cgaaacaagt gctgctgatg gtgaatatcc agcgccgaag
    62281 ttggttcatc aagaaataac catttcggtg agggagtagg ttcccagagt tgcaccagca
    62341 ggcgcgctag ttgcacgcgc tgttgttcac caccggataa ttgccgatag tcgcggtttg
    62401 ctaacgcctg acagtcacat aacgccatta tttgtgctgt ttcattatcc tgattaccag
    62461 tacgatgcgg atgccgcccc atctggataa cttcttgtac gctgaatgga aacgccatgt
    62521 ggctattttg acgcatcacg gcccggtgtt tcgccagttc agtaatcgac cattcattta
    62581 atggcttatt aaacaaccga cattcgccgc tatcaggttg aagatagccg gttaactggc
    62641 gaagcagcgt agattttccc gcaccattag ggccgagaat ggcaacaatt tccccaccgg
    62701 gaaaagtcag cgagacatta tcagtcaaac gccgcccctg aaggctataa accagattct
    62761 gggcgctgat catgtgatcc tccgatttcg cagaatcaac cacataaaat aagggccacc
    62821 aagcaggctg gttaataatc ccacgggcat ttctgctggc tgtaccagcg tgcgggcaag
    62881 cgtgtctgcc atcagcaata aacaggctcc ggcgagggcg gaacaaggga ttagccagcg
    62941 gtgatttgct ccggtagtca tgcgaatcag atgcgggatc accaggccaa taaagccgat
    63001 aatgccgctt accgatacgg cagcaccaac gagcagtgag ctcactaaca gtaattgctg
    63061 gcgtttacgc ttaacgttca cgccgaggta atgggcttct tcatcaccga gctgcagtaa
    63121 attcagcgtt ccggcgagac aagttgttgc gataatggcc ggaaggatga atgaactggc
    63181 aaccaataac gtcggccatt gcgcctgtcc aagattgccc atcatccaca atgtgagttg
    63241 tcgtagctgc tgctcatcgc cgatatagct caggatgccg atcgctgcgc cgcacagggc
    63301 attgatggcg ataccggcga gtaataattg catcatgctg ccatcgcgat ggcgctgcgt
    63361 gatgagaaaa atgatggtgc agactactaa acttccggca atggcaaaca ccatttgctc
    63421 atagagcacc agaaccactg ggaaggagaa gggcagcaca atagcaacgc caaccattaa
    63481 tgcagaaccg ctactgacgc caagtaatcc tgggtcagcc attgggttgc gaaaaagccc
    63541 ctgcataatt acccctgcgg ttgctaacgc tgcacctacc agcaccgcca gcaaaacccg
    63601 tggtagacga atattgagcc agatatcgcg gtattcctca tcaaaaaggg catcaaaact
    63661 gatctttaac gctccctgac tggcaccgaa cagcaccagg gcaagcaata atagtgatag
    63721 ccccataaag accctggcgg atgaaaatga atccttgagc acgcaaaact ccgttacgac
    63781 attacggcgc ggacatgccg cgccggggta tcacgccggt ttcaggtccg gttcaatcag
    63841 actgtagacc tgctggttaa gtgctggcgc atttgccagt tcatggatat gtgcggcgac
    63901 atcggcacgg cggacaaaac catgacactc ctgattttgt acccgttgtg ctttgcctgt
    63961 tgctgcgccg tcaagcagac caccagggcg gagaatggcg tagtctagct ggctggtttg
    64021 cagccagctt tccgccagcg ttttttcccg taccgcctga ccaaacgcag cctttgcccg
    64081 ctccgataaa aacggccagc tatcgccgca tcccaaagag gtcaccagaa tcatgcgact
    64141 gatgccggcc ttttcggcct catcaatcac tgttctgtgc gccagataat cctgagctcc
    64201 tcccattgtt gagatgataa gcgcatccgg gccagctgcg cggcaagcag ccgcgactac
    64261 gctggcatca caggcgtcgc ctgtaaaaac ctgtacgcct tgttgcgcca gtttcgtggc
    64321 ggcatcggca tgacgaatga cagcaacgac cggacgttgt tccgccagcg ccagttcaag
    64381 cgttctggca cccacacctt ttccgcctgc gccaaagagt agccacggcg tcatcaggcg
    64441 tgttccttta acgatgccgc cagagtatgg aaggcgctga cttgttcgct caggagctga
    64501 cgatggtcat ccctgccgag gaagattttc agcatggcgc tgccttcttt gttgaaaaag
    64561 agaatggacg cggtgtccat gcccataaat ttacgttcga ttaaggcaat atgcgtgcag
    64621 ttttctgctt tgatatgccc ggacatgccg tgctttccgc gtaggttgaa atagccgtgg
    64681 cgatggaaac ccgaaggcag ttcgccgcta aattcgagga ttacatcggc agtatgtact
    64741 aacgtggtga cgttgcccca ttcgcagacg gtatcccaga cagtatcgaa tttatcgcct
    64801 ggcactacgg tagagaaggg tagatttctc actacctcca gcagtgtggt gttgtattgt
    64861 tcggctacca cctccagcgt accgtccggc tccgttttca aaaattcttg taacgagaca
    64921 tggctcataa atcactcctg ttatggtttt tcacgcacaa tcgctggcgc attaagtacc
    64981 tgaattagtt cattaagaga ctgcaaaata ttgcttgccc agaaacgacc ttcattagtc
    65041 agccgcaggc aagttgaagc atcgcgactt aagccttttt ggtgccattg agccagtaac
    65101 ggcgcgagtt tttcggcatg tggcgtaagt tcatccagcg gtacacgcgc tgtttcaaca
    65161 cctgactgca aggtatgacg ccattgcgca ttacgttcgg cgttacgcat gatcatcatc
    65221 agcggttttt tgcctgccgc gacggattca tgccaggtct gaagattgcg ttcgttcatc
    65281 caggagtaac cattaatcga accgccggct ccggaaccaa aggccagaca atcggcacct
    65341 tgttttatca gcaggttata gagattgcgt tcgcgtgtgg tacggcccca gtggctgtta
    65401 ctaatacagc gccaaccggc ttcgtccatg aagtcacacc cttgcagata aagatcgcga
    65461 cgttctgccg gagagggcac ggtagtacgc ccattttcca cggctttgcc cagcggtgta
    65521 ttgggcagga cattgagcgc atagagatcg acgccatcga gaccgatatc gcgggcaata
    65581 gccagatctt ctccccaggt ttgcgcatcc tgacccggca ggccaaaaag caggtcacag
    65641 accaccgcag cacggtcacg tttgaccagg ctttccataa acgcaatagc ggttgggcca
    65701 tccgaggtgc gggccatttt cttgcggatt ttgctgttaa acgactggat accaattgag
    65761 aagcggtttg ctcctgcatc aaggcaagca tcgattcgct cagcgtcaaa attcaatacc
    65821 cgtccttcga tagtgatttc acaatcaggg gccagtggca gcttttctcg taatgtggtg
    65881 atgattctgg ccaaatcgtg tgccgaaagt gcggaaggcg taccgccacc gaaatagacc
    65941 gcgtggatgg gggcagactg atgcaatacg ctgtccgctt ccatttctat ttcacgaatc
    66001 agggcgtcgg tataatgagc acacgcatct tcattaaaac ggttctgata aaaaccacaa
    66061 aacgtgcagt gtgtcgcaca aaaaggaatg tgtaggtaaa ccagtcgctt acgaggtgat
    66121 gccgtttgat tgatcatctc ttgccaggtc tgtgccagtt gctctttggc aactggaatg
    66181 gccccacgaa acggcatcat ggcgcgcctg tctttaaatg gctgatcacc gtctaaagca
    66241 aaatgtggcg tgagatccag ggtgttattt gcgttcattc gtctgattta aatccattta
    66301 ttgcggccaa agctgctggt gtaaagactc aacgacatca gtaatacgcg gacccattcc
    66361 cagaatcagg ttttgatcaa cggtgataat gcgctggttt ttccatgcgg cagtgtgggt
    66421 aattccagca atagaacgta gccgattaat atcgccatcg accatttgtg aggttacgac
    66481 aatcacttca gggttagccg caatcagtga ttctgcactg tagcttttgt actgctggtg
    66541 gggagcgacg ttttctgctc cggcaagcga aagaatggcg tccgcgacgc tgcctttacc
    66601 ggcaacttgt ggcgcacttc cgcctgcgga gagaataaac atggctttaa ccggggcttt
    66661 tttggccgcc acgttttgct gtactcgctc caggcgttgg ctgatttgtg tcaccagcgc
    66721 ctcgccttgt tcaggaacct gtagcgtttt tgccagttgg cgaatgttgg cgtacatctg
    66781 ctcaagggtg gctggcacac gcggcagggt gacgacgttg actttctgcg ctcgtagttg
    66841 gtcgagcaca atttgtggcc cggcatcctg ccaggtaatt acgctatccg ggcgaagtga
    66901 caaaatgcct tcgctgctga gttgtttcca gtagccaata tgcggcagtt tggcggtttc
    66961 tggtggataa gatgtcgttt catcgacacc aaccacgcgt tcgccagcgc ccatcgcgta
    67021 gattagctcc gtcagcgatc ctcctgcgac cacgatacgt tcggcagccg ttacgctaaa
    67081 agcccagcta agagccagta ctgccgtaaa caattttttt cgttttactg cggtcttcgc
    67141 ggcagaggcc acggctgaaa tacataacgt cagtggagaa aaaagaaacg gcctggtgat
    67201 gatccttggc ataatatgag aactcttatc aaattgagaa tgatcatgat tatcatttga
    67261 gttatatatg aaatacaacc gtcgattcaa tacgcaaggc gttacaagcg tatttatttt
    67321 tgatttttat caagatgctt cgtcagctaa cgttcttgtt accgactaaa tttcatcgcg
    67381 ggaaaaagct gagaacccag agatatcgag gcttgcagag caaagagaag aaaaaattat
    67441 cactgttaac gacaaaattt agtgatgagg catgggggga taaccataaa aacaaggtgg
    67501 tttacagggc gcaggatagg tttcataacc ccctgtttat tgtgttaatg gtggttaaaa
    67561 aaaacttgat cataaaaaat tgatcgataa taattctcat tatcacgttt gcatttaaca
    67621 aaacacgacc atatggataa caaaaccata gcatttgcaa gggataacgc acagctctct
    67681 tcactcctga tggggcatat cgttttgcct gacaaagagg gcgtgctttt gcgtaattgc
    67741 cgagttatca ggcaatttca tgggatataa acgcctgtaa actcacccgg cgaatattat
    67801 cgctctgaat tttaaataaa tttatttttc gccagccacc ccggtattaa cccaggcagc
    67861 cagcattttt tattttcctc atggagaatc gctatgtcac gtccgcaatt tacctcgttg
    67921 cgtttgagtt tattggcctt ggctgtttct gccaccttgc caacgtttgc ttttgctact
    67981 gaaaccatga ccgttacggc aacggggaat gcccgtagtt ccttcgaagc gcctatgatg
    68041 gtcagcgtca tcgacacttc cgctcctgaa aatcaaacgg ctacttcagc caccgatctg
    68101 ctgcgtcatg ttcctggaat tactctggat ggtaccggac gaaccaacgg tcaggatgta
    68161 aatatgcgtg gctatgatca tcgcggcgtg ctggttctta tcgatggtgt tcgccaggga
    68221 acggataccg gacacctgaa tggcactttt ctcgatccgg cgctgatcaa gcgtgttgag
    68281 attgttcgcg gaccttcagc attactgtat ggcagtggcg cgctgggtgg agtgatatcc
    68341 tacgatacgg tcgatgcaaa agatttattg caggaaggac aaagcagtgg ttttcgtgtc
    68401 tttggtactg gcggcacggg ggaccatagc ctgggattag gcgcgagcgc gtttgggcga
    68461 actgaaaatc tggatggtat tgtggcctgg tccagtcgcg atcggggtga tttacgccag
    68521 agcaatggtg aaaccgcgcc gaatgacgag tccattaata acatgctggc gaaagggacc
    68581 tggcaaattg attcagccca gtctctgagc ggtttagtgc gttactacaa caacgacgcg
    68641 cgtgaaccaa aaaatccgca gaccgttgag gcttctgaaa gcagcaaccc gatggttgat
    68701 cgttcaacaa ttcaacgcga tgcgcagctt tcttataaac tcgccccgca gggcaacgac
    68761 tggttaaatg cagatgcaaa aatttattgg tcggaagtcc gtattaatgc gcaaaacacg
    68821 gggagttccg gcgagtatcg tgaacagata acaaaaggag ccaggctgga gaaccgttcc
    68881 actctctttg ccgacagttt cgcttctcac ttactgacat atggcggtga gtattatcgt
    68941 caggaacaac atccgggcgg cgcgacgacg ggcttcccgc aagcaaaaat cgattttagc
    69001 tccggctggc tacaggatga gatcacctta cgcgatctgc cgattaccct gcttggcgga
    69061 acccgctatg acagttatcg cggtagcagt gacggttaca aagatgttga tgccgacaaa
    69121 tggtcatctc gtgcggggat gactatcaat ccgactaact ggctgatgtt atttggctca
    69181 tatgcccagg cattccgcgc cccgacgatg ggcgaaatgt ataacgattc taagcacttc
    69241 tcgattggtc gcttctatac caactattgg gtgccaaacc cgaacttacg tccggaaact
    69301 aacgaaactc aggagtacgg ttttgggctg cgttttgatg acctgatgtt gtccaatgat
    69361 gctctggaat ttaaagccag ctactttgat accaaagcga aggattacat ctccacgacc
    69421 gtcgatttcg cggcggcgac gactatgtcg tataacgtcc cgaacgccaa aatctggggc
    69481 tgggatgtga tgacgaaata taccactgat ctgtttagcc ttgatgtggc ctataaccgt
    69541 acccgcggca aagacaccga taccggcgaa tacatctcca gcattaaccc ggataccgtt
    69601 accagcactc tgaatattcc gatcgctcac agtggcttct ctgttgggtg ggttggtacg
    69661 tttgccgatc gctcaacaca tatcagcagc agttacagca aacaaccagg ctatggcgtg
    69721 aatgatttct acgtcagtta tcaaggacaa caggcgctca aaggtatgac cactactttg
    69781 gtgttgggta acgctttcga caaagagtac tggtcgccgc aaggcatccc acaggatggt
    69841 cgtaacggaa aaattttcgt gagttatcaa tggtaatcat ctgccccgat atttcggggc
    69901 atttatctgg aaggaagaga gacaatgaac cactacacac gctggcttga gttaaaagaa
    69961 caaaatcccg gaaagtacgc gcgtgacatc gcaggtttaa tgaatatcag cgaagcagaa
    70021 ctggcatttg cacgcgtcac gcacgatgcg tggcggatgc acggcgatat ccgtgaaatt
    70081 ctggcggcgc tcgaaagtgt tggcgaaacc aaatgtattt gtcgtaatga atatgcagtc
    70141 catgagcaag ttggtacgtt cacaaaccag catttgaacg gacatgccgg attgatcctc
    70201 aatccgcgcg cgctggattt acgtctgttt ctcaatcaat gggccagtgt tttccacatc
    70261 aaagaaaaca cggctcgtgg cgaacgccag agtattcagt tctttgatca tcagggcgat
    70321 gcattactaa aagtttatgc caccgacaat accgatatgg cggcatggag tgagcttctg
    70381 gcacggttta tcaccgatga gaatacgccg cttgagttaa aagccgttga tgcgccagtt
    70441 gttcaaacgc gagccgatgc cactgtggtc gagcaagagt ggcgggcgat gaccgacgtt
    70501 catcagtttt ttacgttgct caagcgccac aacctgacgc gccaacaggc gttcaatctg
    70561 gtggcagacg atttggcctg caaagtatcc aacagtgcgt tggcgcaaat tcttgaatct
    70621 gcacagcagg atggtaatga aatcatggtg tttgttggca accgtggctg cgtacagatt
    70681 ttcaccggtg tggtagaaaa agtggtgcca atgaaaggtt ggctgaatat tttcaacccg
    70741 acgtttactc ttcatctatt agaagagagc attgctgaag cctgggttac ccgtaaaccg
    70801 accagcgatg gctacgtaac cagtctggaa ttgtttgccc atgatggtac gcagatagcg
    70861 caactttatg gtcaacgtac agaaggcgaa caggagcaag cgcaatggcg taagcaaatt
    70921 gcttcgctga taccggaagg cgttgctgta taacgtataa ttaacgggag gtctggctat
    70981 ttattgctgg gcctctttat attgatcaat cgttcacacc agataatcaa tatgctgatg
    71041 gcgtaccagc tcattgatcc gcttaagatt gaggatcatc atgatattgt gtttatggca
    71101 gtagaacgtt tttagcgata ttttcagttt ctccagaata tcttccgttg tcatttgatt
    71161 cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt gacacattcg cttcaggcac
    71221 agagcagtaa ctgccattga tgagtgcgac aaaatcaatg atagtgatag tccgtggaac
    71281 aaaggtcacc ggcactttcc ccaggcagcg tttaatatta aagggggaga aaataatgac
    71341 atggacaggt tttaatcgtt caatttgagt gagaaaatta gaatgaaata cattattcat
    71401 taatgtatca ataatgacga aggcattctg atgcaacatt acgtcgatct cttcttcgtt
    71461 ctgtatatga acgacattac ctttactcag aatgtttttc atcgcggtga agaacatcgt
    71521 atccctggta attataagaa acataaccga ctcctggtgc ggacattaac tttataatca
    71581 gtttgccaaa ggtaattaac aggacagggc ttataataat aatagttagc ttactgtata
    71641 ttaattttat gtaatacatg atgcagatgc tgagaaagca tctgcatctt tcggtggtgt
    71701 tatttgacca gctcaggtgt tacctgactc accgcattgg tgtagtaagg cgcaccccag
    71761 cccggttccg gccagaatgc accatagccg taatcccaca ggttataggt ggtattaacc
    71821 acttctctca aatgccacac ctggatgccc tgcatattca cttccaggaa gttatacggg
    71881 actttattga taaagccagg ttgttcaccc tgaatggtgc cgaggatggt aacaaagtga
    71941 ttacgatagt tcactggatc aaggaagccg ctttgtctgg cgagcagtcg gccctgatag
    72001 ttggcttcaa tatcaggctt cgcatagcta tccaacggta atacagcgat ttctaacaac
    72061 gtatccgttt tgccattgat aacgttgata accttcccac caaagcgcgc ttgttgacca
    72121 acatataacc ccggctggtt atgaacagca acaaaacttt tttgaatatc aggttggtta
    72181 ttgcctttga tattttgcgg aattgaacta catgcggcaa gcaaaaatga aaggctgagt
    72241 atgagtgcac cttttgtcat gttcatgtta ctatccttat caacaaatat taaaaccata
    72301 atagatattt ctttatagtt ttcatctgat tctgaggcga tatgcctgat agtaacgtaa
    72361 acctgtctga tgtttatatc ccttctgttt agttcaattc cgttattttg gggcgtggca
    72421 tgagtatcca tcgccccata atataaccct ttgttttaaa attacataaa atctattttt
    72481 attcaatgat tataaagtgc taataatcaa tattatggta ttatattctg ataatgattt
    72541 gataagcgaa ttcttttacc agacggtatt tctatttcca ccccataatt tatatctttt
    72601 tcatcaggaa gtggcctctt taaataaggc gtgcgtgaca ttttttttgg gttaagcgtt
    72661 tcgtattgaa atacttcgaa ttgatattca gacatttctg cccatgtttg aattttattg
    72721 tatcgggaga gtgtcgtaaa ggctttaact tgcttataac attgtttttg gaattcttgc
    72781 tgatatttta atatgtcgtt gtatgaaaga ttggttatcc tggcctctaa aaatttaatt
    72841 attgtcgaag caatttccgc ttttgctcgt aaaaagttgt cattaatggt taataatgtt
    72901 gaaaataaac cttcgatttg tttgtcaaga tattgttcgt ctttatataa ttttggggta
    72961 gggtgttctt tatgtgaaaa aatagtg
//
LOCUS       DAZGCQ010000010        72675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000010 DAZGCQ010000000
VERSION     DAZGCQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.010"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..525
                     /gene="ogt"
                     /locus_tag="ACHK07_000717"
     CDS             10..525
                     /gene="ogt"
                     /locus_tag="ACHK07_000717"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO8101093.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            720..1472
                     /gene="fnr"
                     /locus_tag="ACHK07_000718"
     CDS             720..1472
                     /gene="fnr"
                     /locus_tag="ACHK07_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO8101094.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            1624..2574
                     /gene="uspE"
                     /locus_tag="ACHK07_000719"
     CDS             1624..2574
                     /gene="uspE"
                     /locus_tag="ACHK07_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO8101095.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(2624..2881)
                     /gene="ynaJ"
                     /locus_tag="ACHK07_000720"
     CDS             complement(2624..2881)
                     /gene="ynaJ"
                     /locus_tag="ACHK07_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO8101096.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            3125..4156
                     /gene="ynaI"
                     /locus_tag="ACHK07_000721"
     CDS             3125..4156
                     /gene="ynaI"
                     /locus_tag="ACHK07_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO8101097.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(4207..5820)
                     /gene="mppA"
                     /locus_tag="ACHK07_000722"
     CDS             complement(4207..5820)
                     /gene="mppA"
                     /locus_tag="ACHK07_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO8101098.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(6157..7056)
                     /gene="pgrR"
                     /locus_tag="ACHK07_000723"
     CDS             complement(6157..7056)
                     /gene="pgrR"
                     /locus_tag="ACHK07_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO8101099.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSYPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLRGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            7194..8114
                     /locus_tag="ACHK07_000724"
     CDS             7194..8114
                     /locus_tag="ACHK07_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8101100.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            8114..9043
                     /locus_tag="ACHK07_000725"
     CDS             8114..9043
                     /locus_tag="ACHK07_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8101101.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            9064..9702
                     /locus_tag="ACHK07_000726"
     CDS             9064..9702
                     /locus_tag="ACHK07_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8101102.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            9993..10721
                     /gene="mpaA"
                     /locus_tag="ACHK07_000727"
     CDS             9993..10721
                     /gene="mpaA"
                     /locus_tag="ACHK07_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO8101103.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(10696..11661)
                     /gene="ycjG"
                     /locus_tag="ACHK07_000728"
     CDS             complement(10696..11661)
                     /gene="ycjG"
                     /locus_tag="ACHK07_000728"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO8101104.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQQILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFNLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEQALQFTTGELHL"
     gene            11780..12286
                     /gene="tpx"
                     /locus_tag="ACHK07_000729"
     CDS             11780..12286
                     /gene="tpx"
                     /locus_tag="ACHK07_000729"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO8101105.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(12330..13871)
                     /gene="tyrR"
                     /locus_tag="ACHK07_000730"
     CDS             complement(12330..13871)
                     /gene="tyrR"
                     /locus_tag="ACHK07_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO8101106.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(14019..15080)
                     /gene="ycjF"
                     /locus_tag="ACHK07_000731"
     CDS             complement(14019..15080)
                     /gene="ycjF"
                     /locus_tag="ACHK07_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO8101107.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQVQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(15077..16474)
                     /gene="ycjX"
                     /locus_tag="ACHK07_000732"
     CDS             complement(15077..16474)
                     /gene="ycjX"
                     /locus_tag="ACHK07_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO8101108.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            16629..17627
                     /gene="ycjW"
                     /locus_tag="ACHK07_000733"
     CDS             16629..17627
                     /gene="ycjW"
                     /locus_tag="ACHK07_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8101109.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPHQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMSLK"
     gene            complement(17678..18583)
                     /gene="ompG"
                     /locus_tag="ACHK07_000734"
     CDS             complement(17678..18583)
                     /gene="ompG"
                     /locus_tag="ACHK07_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO8101110.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(18628..19710)
                     /locus_tag="ACHK07_000735"
     CDS             complement(18628..19710)
                     /locus_tag="ACHK07_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8101111.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(19724..20383)
                     /gene="pgmB"
                     /locus_tag="ACHK07_000736"
     CDS             complement(19724..20383)
                     /gene="pgmB"
                     /locus_tag="ACHK07_000736"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO8101112.1"
                     /translation="MKLQGVVFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGDKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLCAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDFLTWSRLSAFWQN
                     V"
     gene            complement(20380..22647)
                     /gene="ycjT"
                     /locus_tag="ACHK07_000737"
     CDS             complement(20380..22647)
                     /gene="ycjT"
                     /locus_tag="ACHK07_000737"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO8101113.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLCLLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGLSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHREDE"
     gene            complement(22644..23699)
                     /gene="ycjS"
                     /locus_tag="ACHK07_000738"
     CDS             complement(22644..23699)
                     /gene="ycjS"
                     /locus_tag="ACHK07_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8101114.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVNPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQARVMRDTARRQGKVLAYDFHHRFALDTQQLREQVTNGILGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(23709..24497)
                     /gene="ycjR"
                     /locus_tag="ACHK07_000739"
     CDS             complement(23709..24497)
                     /gene="ycjR"
                     /locus_tag="ACHK07_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8101115.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEVLAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(24515..25567)
                     /gene="ycjQ"
                     /locus_tag="ACHK07_000740"
     CDS             complement(24515..25567)
                     /gene="ycjQ"
                     /locus_tag="ACHK07_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO8101116.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPAGAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(25598..26440)
                     /gene="ycjP"
                     /locus_tag="ACHK07_000741"
     CDS             complement(25598..26440)
                     /gene="ycjP"
                     /locus_tag="ACHK07_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8101117.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(26427..27308)
                     /gene="ycjO"
                     /locus_tag="ACHK07_000742"
     CDS             complement(26427..27308)
                     /gene="ycjO"
                     /locus_tag="ACHK07_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8101118.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(27329..28621)
                     /gene="ycjN"
                     /locus_tag="ACHK07_000743"
     CDS             complement(27329..28621)
                     /gene="ycjN"
                     /locus_tag="ACHK07_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8101119.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(28635..30314)
                     /gene="ycjM"
                     /locus_tag="ACHK07_000744"
     CDS             complement(28635..30314)
                     /gene="ycjM"
                     /locus_tag="ACHK07_000744"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO8101120.1"
                     /translation="MKQEITDYLAEIYGGTFTSTHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNKITLRPWQVMWIK"
     gene            complement(30524..30838)
                     /gene="pspE"
                     /locus_tag="ACHK07_000745"
     CDS             complement(30524..30838)
                     /gene="pspE"
                     /locus_tag="ACHK07_000745"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO8101121.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(30913..31134)
                     /gene="pspD"
                     /locus_tag="ACHK07_000746"
     CDS             complement(30913..31134)
                     /gene="pspD"
                     /locus_tag="ACHK07_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO8101122.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(31143..31502)
                     /gene="pspC"
                     /locus_tag="ACHK07_000747"
     CDS             complement(31143..31502)
                     /gene="pspC"
                     /locus_tag="ACHK07_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO8101123.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(31502..31726)
                     /gene="pspB"
                     /locus_tag="ACHK07_000748"
     CDS             complement(31502..31726)
                     /gene="pspB"
                     /locus_tag="ACHK07_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO8101124.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(31780..32448)
                     /gene="pspA"
                     /locus_tag="ACHK07_000749"
     CDS             complement(31780..32448)
                     /gene="pspA"
                     /locus_tag="ACHK07_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO8101125.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            32615..33592
                     /gene="pspF"
                     /locus_tag="ACHK07_000750"
     CDS             32615..33592
                     /gene="pspF"
                     /locus_tag="ACHK07_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO8101126.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIILDPFKRRPPEEANAVSENTSLPMLP
                     LDLREFQMQQEKELLQHSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(33712..34977)
                     /gene="puuE"
                     /locus_tag="ACHK07_000751"
     CDS             complement(33712..34977)
                     /gene="puuE"
                     /locus_tag="ACHK07_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO8101127.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(35015..36295)
                     /gene="puuB"
                     /locus_tag="ACHK07_000752"
     CDS             complement(35015..36295)
                     /gene="puuB"
                     /locus_tag="ACHK07_000752"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO8101128.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(36297..37784)
                     /gene="puuC"
                     /locus_tag="ACHK07_000753"
     CDS             complement(36297..37784)
                     /gene="puuC"
                     /locus_tag="ACHK07_000753"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO8101129.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDLDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(37934..38491)
                     /gene="puuR"
                     /locus_tag="ACHK07_000754"
     CDS             complement(37934..38491)
                     /gene="puuR"
                     /locus_tag="ACHK07_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO8101130.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(38518..39282)
                     /gene="puuD"
                     /locus_tag="ACHK07_000755"
     CDS             complement(38518..39282)
                     /gene="puuD"
                     /locus_tag="ACHK07_000755"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO8101131.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            39494..40912
                     /gene="puuA"
                     /locus_tag="ACHK07_000756"
     CDS             39494..40912
                     /gene="puuA"
                     /locus_tag="ACHK07_000756"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO8101132.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            41061..41225
                     /locus_tag="ACHK07_000757"
     CDS             41061..41225
                     /locus_tag="ACHK07_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101133.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            41215..42600
                     /gene="puuP"
                     /locus_tag="ACHK07_000758"
     CDS             41215..42600
                     /gene="puuP"
                     /locus_tag="ACHK07_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO8101134.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            42734..42979
                     /gene="ymjA"
                     /locus_tag="ACHK07_000759"
     CDS             42734..42979
                     /gene="ymjA"
                     /locus_tag="ACHK07_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO8101135.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            43292..44935
                     /gene="sapA"
                     /locus_tag="ACHK07_000760"
     CDS             43292..44935
                     /gene="sapA"
                     /locus_tag="ACHK07_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO8101136.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            44932..45897
                     /gene="sapB"
                     /locus_tag="ACHK07_000761"
     CDS             44932..45897
                     /gene="sapB"
                     /locus_tag="ACHK07_000761"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO8101137.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            45884..46774
                     /gene="sapC"
                     /locus_tag="ACHK07_000762"
     CDS             45884..46774
                     /gene="sapC"
                     /locus_tag="ACHK07_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO8101138.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            46774..47766
                     /gene="sapD"
                     /locus_tag="ACHK07_000763"
     CDS             46774..47766
                     /gene="sapD"
                     /locus_tag="ACHK07_000763"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO8101139.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            47768..48574
                     /gene="sapF"
                     /locus_tag="ACHK07_000764"
     CDS             47768..48574
                     /gene="sapF"
                     /locus_tag="ACHK07_000764"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO8101140.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(48625..49788)
                     /locus_tag="ACHK07_000765"
     CDS             complement(48625..49788)
                     /locus_tag="ACHK07_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO8101141.1"
                     /translation="MARVSPSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(49797..51170)
                     /locus_tag="ACHK07_000766"
     CDS             complement(49797..51170)
                     /locus_tag="ACHK07_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO8101142.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(51174..54281)
                     /locus_tag="ACHK07_000767"
     CDS             complement(51174..54281)
                     /locus_tag="ACHK07_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO8101143.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     SRRQE"
     gene            complement(54281..55402)
                     /locus_tag="ACHK07_000768"
     CDS             complement(54281..55402)
                     /locus_tag="ACHK07_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8101144.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VTELTGRTSAAFSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(55551..56117)
                     /locus_tag="ACHK07_000769"
     CDS             complement(55551..56117)
                     /locus_tag="ACHK07_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8101145.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            56595..57383
                     /gene="fabI"
                     /locus_tag="ACHK07_000770"
     CDS             56595..57383
                     /gene="fabI"
                     /locus_tag="ACHK07_000770"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO8101146.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            57527..58654
                     /gene="yciW"
                     /locus_tag="ACHK07_000771"
     CDS             57527..58654
                     /gene="yciW"
                     /locus_tag="ACHK07_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO8101147.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            58722..60656
                     /gene="rnb"
                     /locus_tag="ACHK07_000772"
     CDS             58722..60656
                     /gene="rnb"
                     /locus_tag="ACHK07_000772"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO8101148.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(60775..60837)
                     /locus_tag="ACHK07_000773"
                     /pseudo
     CDS             complement(60775..60837)
                     /locus_tag="ACHK07_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            60891..62876
                     /gene="pdeR"
                     /locus_tag="ACHK07_000774"
     CDS             60891..62876
                     /gene="pdeR"
                     /locus_tag="ACHK07_000774"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8101149.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(62858..62986)
                     /gene="ymiD"
                     /locus_tag="ACHK07_000775"
     CDS             complement(62858..62986)
                     /gene="ymiD"
                     /locus_tag="ACHK07_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO8101150.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            63024..63197
                     /gene="yciZ"
                     /locus_tag="ACHK07_000776"
     CDS             63024..63197
                     /gene="yciZ"
                     /locus_tag="ACHK07_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO8101151.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            63287..64036
                     /gene="yciT"
                     /locus_tag="ACHK07_000777"
     CDS             63287..64036
                     /gene="yciT"
                     /locus_tag="ACHK07_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO8101152.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            64305..64523
                     /gene="osmB"
                     /locus_tag="ACHK07_000778"
     CDS             64305..64523
                     /gene="osmB"
                     /locus_tag="ACHK07_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO8101153.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(64649..64975)
                     /gene="yciH"
                     /locus_tag="ACHK07_000779"
     CDS             complement(64649..64975)
                     /gene="yciH"
                     /locus_tag="ACHK07_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO8101154.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAPPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDIDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(64975..65712)
                     /gene="pyrF"
                     /locus_tag="ACHK07_000780"
     CDS             complement(64975..65712)
                     /gene="pyrF"
                     /locus_tag="ACHK07_000780"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO8101155.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(65905..67074)
                     /gene="lapB"
                     /locus_tag="ACHK07_000781"
     CDS             complement(65905..67074)
                     /gene="lapB"
                     /locus_tag="ACHK07_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO8101156.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(67081..67389)
                     /gene="lapA"
                     /locus_tag="ACHK07_000782"
     CDS             complement(67081..67389)
                     /gene="lapA"
                     /locus_tag="ACHK07_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO8101157.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(67538..68302)
                     /gene="pgpB"
                     /locus_tag="ACHK07_000783"
     CDS             complement(67538..68302)
                     /gene="pgpB"
                     /locus_tag="ACHK07_000783"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO8101158.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            68472..69062
                     /gene="ribA"
                     /locus_tag="ACHK07_000784"
     CDS             68472..69062
                     /gene="ribA"
                     /locus_tag="ACHK07_000784"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO8101159.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(69126..71801)
                     /gene="acnA"
                     /locus_tag="ACHK07_000785"
     CDS             complement(69126..71801)
                     /gene="acnA"
                     /locus_tag="ACHK07_000785"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO8101160.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(71965..72060)
                     /gene="ymiC"
                     /locus_tag="ACHK07_000786"
     CDS             complement(71965..72060)
                     /gene="ymiC"
                     /locus_tag="ACHK07_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO8101161.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(72174..72341)
                     /gene="yciX"
                     /locus_tag="ACHK07_000787"
     CDS             complement(72174..72341)
                     /gene="yciX"
                     /locus_tag="ACHK07_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO8101162.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(72344..72484)
                     /gene="ymiA"
                     /locus_tag="ACHK07_000788"
     CDS             complement(72344..72484)
                     /gene="ymiA"
                     /locus_tag="ACHK07_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO8101163.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        17780 a        19074 c        17800 g        18021 t
ORIGIN      
        1 agagaacgga tgctgagatt acttgaagaa aaaattgcca cgccactggg tccactgtgg
       61 gtgatttgcg atgagcaatt tcgcctgcgg gcggttgaat gggaagagta cagcgaacgc
      121 atggtgcagc tgctggacat ccattatcgc aaagaaggct atgagcgcat ttctgccacc
      181 aatccaggcg gtttaagcga caagcttcgt gaatattttg ccggtaatct tagcattatt
      241 gatacgcttc ccactgctac gggggggacg ccatttcagc gcgaagtctg gaaaacacta
      301 cgcactatcc cctgcgggca ggtaatgcat tacggcgaac tggctgagca attgggccgt
      361 cctggcgcgg cgcgtgccgt tggtgcggca aacggatcga atcccatcag catcgtcgta
      421 ccttgccatc gggttattgg ccgaaacggc accatgaccg gatatgcagg cggagttcag
      481 cgaaaagagt ggttattgcg ccacgaaggt tatcttttgc tgtaaacatt aaacaatttg
      541 tgccagcttg ttcacacttt tatgtaaagt tacccttaac aacttaaggg ttttcaaata
      601 gatagacata tatttacatc taatatcgga attctctgct gttaaggttt gcttagactt
      661 acttgctccc taaaaagatg ttaaaattga caaatatcaa ttacggcttg agcagaccta
      721 tgatcccgga aaagcgaatt atacggcgca ttcagtctgg cggttgtgct atccattgcc
      781 aggattgcag catcagccag ctttgcatcc cgttcacact caacgaacat gagcttgatc
      841 agcttgataa tatcattgag cggaagaagc ctattcagaa aggccagacg ctgtttaagg
      901 ctggtgatga acttaaatcg ctttatgcca tccgctccgg tacgattaaa agttatacca
      961 tcactgagca aggcgacgag caaatcactg gtttccattt agcaggcgac ctggtgggat
     1021 ttgacgccat cggcagcggc catcacccga gcttcgcgca ggcgctggaa acctcgatgg
     1081 tatgtgaaat cccgttcgaa acgctggacg atttgtccgg taaaatgccg aatctgcgtc
     1141 agcagatgat gcgtctgatg agcggtgaaa tcaaaggcga tcaggacatg atcctgctgt
     1201 tgtcgaagaa aaatgccgag gaacgtctgg ctgcattcat ctacaacctg tcccgtcgtt
     1261 ttgcccaacg cggcttctcc cctcgtgaat tccgcctgac gatgactcgt ggcgatatcg
     1321 gtaactatct gggcctgacg gtagaaacca tcagccgtct gctgggtcgc ttccagaaaa
     1381 gcggtatgct ggcagtcaaa ggtaaataca tcaccatcga aaataacgat gcgctggccc
     1441 agcttgctgg tcatacgcgt aacgttgcct gatttttccg cataactcac tatccttctg
     1501 tcatatcatt aaatttttct gatttattga tctggcagaa ggttcatcac tgtttcattc
     1561 accagatatg ggttaatctt ttaattacaa actgcgttga cagttgttgt aaggagaccc
     1621 tgtatggcta tgtatcagaa catgctcgtt gttatcgatc ctaaccagga cgaccaacca
     1681 gcattgcggc gagctgttta tttacatcaa cggattggtg gcaaaattaa agcctttttg
     1741 ccgatctatg acttctcata cgaaatgacc accctgctct ccccggacga acgtaccgct
     1801 atgcgtcagg gcgtcatcag ccagcgtaca gcctggatcc acgagcaggc aaaatattat
     1861 ctcaatgctg gcgttcccat tgaaattaaa gtggtctggc ataaccgtcc tttcgaagcc
     1921 atcattcagg aagtgatcag cggcgggcac gatttggtgc taaaaatggc gcaccaacat
     1981 gaccgtctgg aagcggtgat ttttacgcca acggactggc atctgttacg caaatgccca
     2041 agcccggtgt ggatggtgaa agaccagccg tggccggaag gaggtaaggc gctggtggcg
     2101 gtgaatctcg ccagtgaaga gccgtatcat aatgcgctca atgaaaaact ggtcaaagag
     2161 acgatcgaac tggcagaaca agtcaaccat accgaagttc atctggttgg cgcttaccca
     2221 gtaacgccaa ttaatatcgc gattgaactg ccggaatttg atccgagcgt ttataacgat
     2281 gccattcgtg ggcaacattt gctggcaatg aaagctctgc ggcagaaatt cggcattaat
     2341 gaaaacatga cgcacgtaga aaaaggtctg ccagaagagg tgattcctga tttggcggag
     2401 cacttacagg cggggattgt ggttctgggc acggtaggac gcaccggtat ttcagcagca
     2461 ttcctcggca acacggcgga acaggtgatt gatcatcttc gctgcgatct gttggtcatt
     2521 aaacctgacc agtatcagac acccgttgaa ctggatgacg aagaagacga ttaacgactc
     2581 ttcaataact gacaaggccc gagttatcgg gcctattttt tacttactgc tcttcgccgc
     2641 caaggaaata aattcccaac gggattgcca gcaacacagt aaagaccagg ctataaacaa
     2701 aaatcatcga tgactgaatc acatacattg atgtcgtcca ctcagacagc ggaatattgt
     2761 actgttctat aacgccgccg atagtcgcgc gagtcacaac cgacgccgca ataatgaaaa
     2821 ccgccaacaa accaaagaga aatttcttcc ctttcgctga cttcagtttc gccataatca
     2881 tgatgtaatc cttgtgcaga caaatttctt gttacctaaa cgtaacaata ccatgacatt
     2941 tcaccgttgt ctgctctgaa aacctcacct cttagctgga tttacaatca acgcattgat
     3001 tagttagcta atgttgctac ttccttccct gactattgat gatctgaatg aattgtcgcg
     3061 gtaaaatagc cccgccaggg gtttccggca tgacgacaat tatctggagg agcctgataa
     3121 aacaatgatc gctgaactgt ttacaaataa tgcgcttaat ctggtcatta ttttcggtag
     3181 ctgcgcagca ttgattctga tgagcttttg gtttcgccgc ggaaatcgta aaagaaaagg
     3241 ctttttattc catgcggtgc aatttttaat ctacaccata attatcagtg ctgttggtag
     3301 cattattaat tatgttatag aaaactataa actcaaattt atcaccccag gcgttatcga
     3361 ttttatctgt acgtccctga ttgcggttat tctgacgatt aagctatttt tgctgattaa
     3421 tcagtttgaa aaacagcaga ttaaaaaagg tcgcgatatc accagtgcgc ggattatgtc
     3481 gcgcattatc aaaatcacca ttattgtggt gcttgttctg ctttatggcg aacatttcgg
     3541 catgagcctt tctggcttgc tgacctttgg tggtattggt ggtctggctg tcggtatggc
     3601 cggtaaagat attctgagta atttcttttc cgggatcatg ctctatttcg accgtccttt
     3661 cagtattggc gactggatcc gttcaccgga cagaaatatc gaaggtacag tagcggaaat
     3721 tggctggcga attaccaaaa ttacgacctt tgataatcgt ccattgtacg taccgaactc
     3781 gctgttttcg tcgatcagcg tagaaaaccc aggacgaatg accaaccgcc gcattaccac
     3841 gaccattggt ttacgttatg aggatgcggc aaaagtgggc gttatcgtcg aagccgtacg
     3901 tgagatgctg aaaaatcacc cggccatcga ccagcgacaa accttactgg tttatttcaa
     3961 ccagtttgct gactcgtcat tgaatattat ggtttattgc tttaccaaaa ccacggtatg
     4021 ggctgagtgg cttgctgcac agcaagacgt ttatttgaag attatcgata ttgtacagtc
     4081 acacggcgcg gactttgcct tccccagcca gacgctgtat atggataaca ttacaccgcc
     4141 ggaacagggt cgctaaatca gacatcactg gcgttatcca caagggtaac gccagttccc
     4201 atcacatcaa tgcttcacaa tatacatagt ccgactgtac gccacatctt cagggttatt
     4261 aatgggataa cctttcagcc acggtttgat taatcgtcca ttggtatatt gataaattgg
     4321 tgcaatcggt gcttgctcca tgaggatttt ttctgccgcg ttgtaatcgg cattacgcgc
     4381 tttaacggta ttttccgtcg atgcctgggc cagaactttg tcgtatgccg gattgttaaa
     4441 gcgtgaaata tttcctgaat gcgttgacgt taataatgtc aggaaagtgg agggttcatt
     4501 ataatccccc acccatgagg cgcggataac atcaaaattg ccggtgttac ggctatcgat
     4561 ataggttttc cattcctgat tttgcaattt aacatctacg ccaaggttct ttttccacat
     4621 cgatgctaca gcaatcgcaa ttttttgatg gttttctgaa gtgttataca aaagcgtcag
     4681 cttcagcggt ttttgcggac cataaccagc tgcgctcaac aaagtttttg cctgcgcatt
     4741 cagttcttcc tgactcattt gttcaaacgg cgaaggttcc ggcgtaaatc cggcggtaac
     4801 atctggtgta aaatgccacg ctggcttttc gcccgtccct aatacttttt cggtcatcag
     4861 gcggcgatct atcgtcatac ttaatgccag acgaacgcgc tgatctgccg tcggaccttt
     4921 ttgcgtgtta aacgcataat aataggtccc gagctgcggc ggcgtataaa cctgccccgg
     4981 aatatccttc aacagcttct gatacatatt tttcgggaag gattcggtga tatcaatatc
     5041 ccccgcgagg taacgcttag tggctgcgga ttcctgatta attggcagga aggtcacttt
     5101 ttgcagtacc gttttggcgt tatcccaata atgggtattc ggtaccacga ccagtttttc
     5161 attgactacg cgttctttaa gaacataagc gccattgccg atcagatttc cggatttcgt
     5221 ccactcttta ccgctttcta cgttggcttt ttgcaccggg aagaaggcaa agttagcggt
     5281 taaattcaca aaccacggca acggtttatc aagctgaatt ttcaaagtat gggcatcaac
     5341 tgcggtgacg ccaagctggt caggcgtagc tttaccatca ataatcgcct gtgcgttgtt
     5401 gattcccgcc agcgcggcaa accatgcaaa tggcgatagc gttttcgggt ccaccagacg
     5461 ctgccagctg tagacaaaat cttgcgccgt taccggtgtg ccatccgccc attttgcgtt
     5521 atcgcgcagg gtaaaagtcc agatacggtt gtcattactt ttccactgag tcgcaacgcc
     5581 ggggacaatt tcccctttct cgttctgatt caccagtcct tcaaacagat cgcgaatgac
     5641 ctgaatctct ggcaggccca cggctttagc gggatccagc gacgcaggct catctttaat
     5701 gtggcgcacc agctcctgct tctctgccag tactgtgccg ctcggaactt ctgcagcata
     5761 cgaaagagaa atgctgctga ccaacagcgc acaacacgtg actgaaacag agtgcttcat
     5821 aagtttccct ctaaccgaca ttaaaattga tgcgtaatta tttgtttcgc cttaaagaaa
     5881 tgcaaacaac ttccatcaaa aaagtgacac aggaagcata aaatgtttta tgtatcaata
     5941 tattaaatca gatttagtgt aaataatcga cgccaaaatg cggcaaagca catagaacac
     6001 cgaacactac gttcgggctt cgcacgttca aaccagtttt cggtatcata atcagcatct
     6061 gtcgccattg tacgaagaat aaaaaataac caaaacgttg aaaggaactg agaatacgtt
     6121 aagagaagaa ttaaattaat gtatcagcaa gtagtgctaa acagcacctt tatacttcag
     6181 cctatctata aataatgaga atgccgagcc agcatgtcga cggtgtggat agtacaggtg
     6241 ataaccgcgt aaatctggtg tgaatttatc caaaacacgt atcagttttt tttctttaat
     6301 agcccgctca acctgatcat aaggtagata cgccaatcca tgcccatcaa ttgcagcatc
     6361 aatgatcagg tctatcgtat tcagtaaaag ctgaccttcc atgcgaacac gaacttcacg
     6421 tccaccgcgt attaatctcc agcgatttgc tgtacccgat gtgggaagat acaaattaat
     6481 tgcctgatga tctattaatt gtgacactga cgttggaaca cttcggcgag aaaaataatc
     6541 cggtgagcca acaatagcca ttggtatatc tggcccgatt cgaatggcga tcatatcttt
     6601 atccatctcc ccacccagac ggacgcctgc atcaaaacgt tcagaaacga catcggtcaa
     6661 accataatca atggtgagct gaatatcaat ttcaggatac gatttcagga atgtgcgcat
     6721 tgctggtaac aatatcgttt ttgctgcatg ttctacagta gtaatacgta ttgtcccgga
     6781 tgggcggttc tgcagatcgc tcagggatgt cagggcagaa tctatgtcat gcaacatagg
     6841 gccaagaaca gacaaaagat gctcgcccgc ttcagtgggt acaacgctgc gcgtagttcg
     6901 cgtcagaagc cgcaatccca atcgttcttc tatacggcgc actatctggc ttaaagctga
     6961 ctgcgccatg ctcaggcggg ctgctgcgcg agtgaagcta cgctcctctg caacgacgac
     7021 aaacgccatc agatcagcga tttcttcacg cttcattcag tatctccata caatctatta
     7081 atcatttaaa tgataggtct aatcatgatt ttgggtctaa tcaacttaat gatcaatatc
     7141 tatagtgata tcaccaaccg ttaaaacgag ccaacaactc catgaggaac ggaatgatga
     7201 ataataaagt cagcttcact aacagcaata atccaaccat ctctttgtct gcagtgatct
     7261 attttcctcc aaaatttgat gaaacccgtc aatatccggc aattgtggta tctcacccag
     7321 gtgggggcgt taaagaacaa acggccggga cctatgccga aaaactggcg gaaaagggat
     7381 ttgtcactat tgcttatgac gcatcttatc agggtgaaag tggcggcgag ccgcgtcagt
     7441 tagaaaaccc atacattcgt actgaagaca tcagtgccgt gattgattac ctgacaacgc
     7501 tttcttatgt cgacaatacc cgaattggtg cgatgggaat ttgtgccggt gcaggatata
     7561 cagccaatgc tgcaattcag gatcgccgca tcaaggccat cggtaccgtt agtgcggtca
     7621 atattggttc gatgttccgt aatggttggg aaaacaatgt gaaatctatt gatgccctgc
     7681 catacgttga agcgggttca aacgccagaa ccagcgatat tagcagcgga gaatacgcca
     7741 ttatgccact ggcaccaatg aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct
     7801 gggagtatta ccacacccct cgcgcgcagt atccaacagc accgggttac gctactttgc
     7861 gcagccttaa ccagattatt acctatgatg cttaccatat ggcggaagtg tacctgactc
     7921 agccgacgca gattgtggcg ggcagccagg cagggagcaa atggatgagt gacgacctgt
     7981 acgatcgggc atcaagccag gataaacgct atcacattgt tgaaggcgca aaccatatgg
     8041 atctttatga cggcaaagcc tatgtggctg aagcaatttc agtattagct ccgttctttg
     8101 aggaaactct gtaatgaaac atattcaaat ccgcaatagt gatatggcct ggcatattgc
     8161 tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg cgattatcag
     8221 cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg gcaaggcatt
     8281 ggctgaaaaa ggctatgtcg ttctcgctta tgacgccagt ttccagggag aatctggtgg
     8341 ggagcctcgt tggatagaag atccgacaca gcgcgtagag gatattagcc gtgttatcga
     8401 ttatgcagta actctgccat atgttgatgc tgaacgcatt ggcgtgctgg gtgtgtgtgg
     8461 ggggggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag ctgtagtgag
     8521 tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt acgatccgat
     8581 tggcgcattg aatgcaatgg cgtcgcaacg aaccaaagag gctcgtggtg gagaattaca
     8641 gatcaacgaa ttacttcccg ccagcctgga tgcagcaaaa gcccatggac tgaccgaacg
     8701 cgatgtttat gaagcaactg actactataa aacaccacgc gggcaacaac cgggaggtgc
     8761 gacaaaaatg ttgttctcac atgcacagaa aacgctggcc tgggatgcct tcgcatttac
     8821 tgaagtgtta ctgactcagc cggtgatggt agtagttggt gaaaaagtcg gtgccttcgg
     8881 tgcttatcgt gatggactgg aagtctatgg ccgggcgatg gtttcgcagg atcgtcagtt
     8941 agtttcatta cccgatttct cacattatga actgtatgac aaaccggaag cagttcagga
     9001 ggcgctggaa aaagttattc cttttttcaa cacccatctg tgataagttt catgagaaat
     9061 attatgaaaa atgtactcat tcttggtgct ggcggtcaga tagcccgcca tgtgattaat
     9121 caactggcag ataaacaaac cattaaacaa actttatttg ctcgtcagcc agcaaaaata
     9181 cacaaaccct atccaactaa tagccagatt attatggggg atgtactgaa tcatgcagca
     9241 ctgaagcagg ccatgcaggg acaggatatt gtgtatgcca accttacggg ggaagattta
     9301 gatattcagg caaattcagt gatcgctgcg atgaaagcct gtgatgttaa acgtctgatc
     9361 tttgttcttt ccctcggcat ttatgatgaa gttccaggaa aatttggcga atggaataat
     9421 gcagttattg gtgagcccct aaaaccattc cgcagggctg cggatgcaat tgaggcttct
     9481 ggactggaat atacaattct tcgccctgcc tggctgacgg atgaagatat tattgattat
     9541 gagcttacct cacgcaatga accattcaaa ggtacgatcg tatcacgtaa gagtgtcgca
     9601 gcattaatta cagatatcat cgataaacct gagaaacaca ttggtgagaa tataggcatc
     9661 aatcaacccg ggactgatgg cgacaagcca ttcttcatgt aaatcaatta tatagccacc
     9721 atcgtactgt actatgtgca gtacgattta ttaaacttat taaggtgaac tgatcaatca
     9781 gatgcgatta acatgtgtag aaccagtaag aataaaatac gccatgtata taacattacg
     9841 acaattctaa tatctgcaca tcaatgaaaa tacaaacaac ttccggcaaa aaagtgatac
     9901 aagatcgatg acatagcgtt gaagaaaagg atttgataat ccacaaaatt tgcacaacac
     9961 tgcctgtaat acttcctgaa ccagagacga tcatgaccgt aacccgccca cgcgccgaac
    10021 gcggcgcatt tccgcccgga acagaacatt acggacgttc attattgggc gcgccgttaa
    10081 tctggtttcc ggcccctgca gccagtcgcg aaagtggttt gattctggct ggcactcacg
    10141 gtgatgaaaa ctcttcggtc gtcacgctct cctgtgctct gcggactttg acaccttctt
    10201 tgcgccgcca tcatgtggtg ctgtgtgtga atcctgacgg ctgccagctg gggttacggg
    10261 ccaatgctaa tggtgtggat ttaaacagaa actttccggc ggcgaactgg aaggaaggtg
    10321 aaacggttta tcgctggaac agcgccgctg aagaacgtga tgtcgtatta ctgacaggtg
    10381 acaaacctgg ctccgaacct gaaacccagg cgctgtgcca gcttatacac cgcattcaac
    10441 ctgcctgggt ggtctcattc cacgatccac tggcttgtat tgaagatccc agacacagcg
    10501 aattaggcga atggctggcc caggcgtttg aattgcctct ggtaaccagc gtcggttatg
    10561 aaacgcctgg ctctttcggc agttggtgtg cagatctcaa cttgcattgc atcaccgcag
    10621 agtttccgcc catctcctcc gacgaagcca gcgaaaaata tctctttgcg atggcaaact
    10681 tgctgcgctg gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg cctgttccac
    10741 atctaccgcc agccaggtcg gtccgtcaag atcggcgaaa ctgacctgcg gcaccagcgg
    10801 taaagcggcg ctaatggcac gagaggtaca caacatgcag cccagcatca gattgaaacc
    10861 ttgtgcacgc gcttcagtcg ccagcgccag cgcttccgtc agccccccgg ttttatcgag
    10921 cttaatgtta accatctcat agcgcccttt cagcgccttc agattgctac gagtatgaca
    10981 actttcatca gcacaaatcg gcaacggatg aataaaattc tccagtgccg catcgtcctg
    11041 cgcaggaagc ggttgttcaa gcatcgcaac gcctaaatcc gccaatagct ggcaacgtgc
    11101 cgccaaccct tctgcacgcc aggattcatt tgcatcaacg atcagcgtgg catcgggcac
    11161 agctgtgcga attgccacca tccgctcact gataagatgg ttatccagct tcactttcag
    11221 taatttcgcg ccggcctgcc agagtgttga tgcactattg gccatctgat caggcgtacc
    11281 gatgactacc gtctgtgcag tgataactgt ctcgggaagc gttatgccga tcaaatcagc
    11341 cagcgattgc tgctgttttc gcgcggccag atcccacaat gcacaatcca gcgcattacg
    11401 tgctgcgccg gcagggagaa tttgttgcaa ctcctcccgt gtcagccctt tctctaattg
    11461 cggcacgacg ctcataattt gcgccattac cgaggcatca ctttctccat aacgcggata
    11521 cggcgtgcat tcgccggtgc ctttaatacc ctcttcttcc agttcaacca ccaccacgcg
    11581 cgcttcactg cgacttcccc gggcaatcac aaacggggta tgtaagggcc aggcttcctc
    11641 gaatacctta acggttctca tctttactcc ttgttgtcgc ggtggtctgc ggattaagcg
    11701 caaatagcgt ttgctgtgct attgacagtt agcataaact aggtgtgacg ttaactatat
    11761 gtaaacagga aagataatca tgtcacaaac cgttcatttc cagggcaacc cggttacagt
    11821 cgccaactcc atcccgcagg cgggtagcaa agcgcagact tttactctcg tggcaaaaga
    11881 tctgtctgac gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga acattttccc
    11941 gagtattgat accggtgttt gcgccgcatc agtacgtaag tttaaccaac tggcaaccga
    12001 gatcgacaac accgttgtgc tgtgtatttc tgccgatctg ccgttcgccc agtctcgttt
    12061 ctgcggcgca gaaggtctga acaacgttat caccctctcc actttccgta acgctgaatt
    12121 tctgcaagcc tacggtgtgg caattgctga tggcccactg aaaggtctgg cagcgcgtgc
    12181 cgttgtggtt attgatgaaa atgacaatgt gattttcagt cagctggtgg atgaaatcac
    12241 caccgagccg gattacgaag cagctctggc tgtactgaaa gcataagtta atatgcctga
    12301 tggtgttgca ccatcaggca tattcgcgct tactcttcgt tcttcttctg actcagacca
    12361 tattcccgca acttattggc aatcgcggta tgtgaaacgc cgagacgttt tgccagtttg
    12421 cgcgtgctgg gataattgcg ataaagctgg gttaataccg agcgttcaaa acggctggtg
    12481 atttcatcca gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc ggcgtcataa
    12541 tccggcaaca aaatatcctg tggacgcagt tcataaccgt ccagttgtgt cagtgcgcga
    12601 tagatagcgt tctttaactg ccgcacattt cccggccacg cataacgcgt aagtacagta
    12661 ttcaggtcag cggccagttt cggacgcggc acgccctgct cgtcggcaaa gcgggcgacg
    12721 aacagctcgg ttaacggcat gatgtcctgc gggcagtcgc gtagcggcgg cagattgagc
    12781 gtcaacacgt tcagacgata atagagatct tcacggaaca cgcctttttg caccagttcg
    12841 accagattct tctgcgtagc gcaaatcacc cgcacatcga catgcacctc atggtcttcg
    12901 ccaacccgac ggaaagtgcc atcattaagg aaacgcagta atttcgcctg catccgtggt
    12961 gacatctcac ctatttcatc caacagcacc gaaccaccgt tcgcctgctc aaagaatcct
    13021 ttcttccctt ccggagcatg accaaacagt tcactctcga ccgcatcttc cggtatagac
    13081 gcacagttca gcgccaggta aggtttgctc gctcttgggc ttgcctgatg gcaggcgtag
    13141 gcaaaaagat ctttacctgt acctgtgtca cccgtaatca gcagcggcgc gcttagcatc
    13201 gccagtttct gcgcctgttc gacaacatgc ttcattttcg ggctgacagc gacaatttga
    13261 ctgaaggcgc tgacgtcctg ggcggcgaca ttttgcaact gacggcccat acgaatcgtt
    13321 gatcgcaaca tcaccaccgc acctgtcagg acgtgttgat cattttcatc ctgaagataa
    13381 acaggcgtaa tctccatcag gaagttctgt ccattaataa caacatgctc gttatgggaa
    13441 tcttgcggtt cgctttccag ccaacgtaaa aaattaaagc cgttaatcaa ttgtgcggcg
    13501 gtatggttgc gcaggcgatc caatttttgc ccaaaaagct gacagctcgc cgggttcgcc
    13561 atatccactt tgcttttcat atcgacagag agcacaggtt caggcaacgc ctccagtaac
    13621 gcgctcaacg ccagatgctc acgttcggaa ggcatccacg ggacagtacg cacatcggta
    13681 acacctgcaa tacggcgtat ttcggccatc agactgctga aactctcaaa ctccagttca
    13741 gcaaaattga ggtagattcg cccaatggga tcaatctcaa taccgcgtaa atcaatgcct
    13801 cttagcacga gtagatcgag taattcgcgg gtcagaccga gtcggtcttc acaaaagact
    13861 tccagacgca tgggaacctt cacctgaaaa aagaacaatt aatatgatga tatgacacta
    13921 tttggatagc aggaaggttt ctgtcaacaa tcattgacac aaagacggaa aaacgggcac
    13981 ttcggcgtaa agatatccgg tcagcacccg atattgcatt atttttcgct gggcgttttg
    14041 cctttttgca gcgtttcttt cacctgaccg ataagctgac gacggaaatc cccgaggcga
    14101 ggtttgtcat cgtcaatcca cggcagcggg cggcaaagct ccatagcttt aatcccgagt
    14161 cgtgccgtca gaagcccagc accaatcccc tgagctgcgc gggtagacaa acgagcagcg
    14221 agatcttgcg acatccagtc catccccact tcgcgcacca attcgctggc tccggcgaaa
    14281 gcgatattca gcaataccag cttgaacagg cgcaaacggc tgtaatatcc cagttcaatg
    14341 ccatacagcg tggcgatgcg attaatcaaa cgcagattgc gccaggcgat aaatgccata
    14401 tccaccagcg ccagcgggct gaccgcaatc atcaacgttg attccgctgc cgaacggctg
    14461 atttcgcgcc gcgcctgggc atctaaaact ggctggacca aatgcgcata caggctgacc
    14521 acttcacggt cgttttgcgt ttcatggatt gaggcatacc agcgttgcag cgccggatgc
    14581 gactgatcaa tacccgcctg ctgcgccagt ttttcgcaaa atgcgcggcc tttgccggtt
    14641 ccgtggctat gcaataaatc acgcgcttcg tcgcgttcat gggcgcgctg tcgcaagcgc
    14701 cataagcgcc gccactctgt taccacagaa cctacgccag cgccgataat caatgcccca
    14761 gcggcacatc cacccagcgc cacccagtcc tgagtttgcc aggcattcat tgtccactgt
    14821 ataccctgcc cgacaacgct tgcgccaaac agagccagcc cgcccatcac cattttgcgc
    14881 cacaggctgc gtttcggacg taacgctgcg tccattaccg cttcgacttg cccctcttct
    14941 tcctgcgctt cgtcgagcgt ggccggggca aaattttgca cctgattttc gtcaaaggtc
    15001 tgctgcgccc tgaatttagg attctgatcg acctccagag gaccgtcgaa atcaatacgt
    15061 ggttttaacg gttcggtcat cgcaatttat ctcctattaa aaattccagc gcggcatcaa
    15121 gacgaatatg cggcagcggt ttgtcgacat ccatcacctg cggacgaaac gcctcaaact
    15181 ggaagccttg cttatcccag aacgcctgac caggcaaacg tgcggggact tcgccaggat
    15241 aaacagtgag cggtgcgcca tcactaagtc gattaccgcg cagcgccggg attttctcac
    15301 cgttaacatc aataatgccg ctggtggtcg cctgaacaga cgccagcccc aggcagtcca
    15361 tgctgatccc ttcgaacgcc gcattttgcc aggcatcctg aatcagttgt tgcagtaatg
    15421 aaaccatatt agcgtgctga tcgatggtca catggtccgc tttagtggca gcaaacaata
    15481 gcttatcgat aaccggcgaa aacaaacgcc ggaacagggt acgctgcccg tagtgaaaac
    15541 tttgcatcag ttgcgtcagc gccagacgca tatcattaaa tgcctgtggc ccactgttga
    15601 gaggttgcag gcagtccacc agcacaatct ggcggtcaaa gcgcagaaaa tgattcttat
    15661 agaacccctt caccaccttc tcgcagtaat aattaaaccg ctcgcgcagc attccggcat
    15721 tggtgtgctt atcggcctgc gccagtttgg actcgcccca ggcatcgaca tccggccacg
    15781 ggaagaattg cagcgcgggc gcacctgcca tatctcccgg caagacaaag cgccctggct
    15841 gaataaagtg cagcccctgc tgtttacagt ggtggagata atcggtccac gcggcggcaa
    15901 tgtctgccag ccggttttcg tcggcaggcg ctagcgggtc cagcccttcg cacatcattc
    15961 gccatttcgc cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc tggcgcgacc
    16021 agcttaaata gtcctgcgcc agcatcggca ggtcgagcaa ccattcgccg gggtaatcca
    16081 caatctccag atacagcgtg gaggtatcct taaagtggcg cagcagcgaa tcgttcgatt
    16141 tatagcgtag cgccaggcga atttcgctga cgccgcgcgt tggcgtcggc caggcgggag
    16201 gatcgccata cagctgcgcc aacccttcgt cgtaggtaaa acgcggaatg ccaaagtcac
    16261 gctgaggaat gcgttttacg cccagcaggc gctcttcacg caccgcactt aacagcggca
    16321 aacgtgctcc ggcatgaata ttgagcaact gattaaccat cgcggtgata aacgctgttt
    16381 tgccgctgcg gctaagtccg gttacagcga ggcgcagatg tctgtcgaca ccccgattca
    16441 ccagcgcatt aagttcattt ttaagtcgct tcattgctgt cctgtgctgc tctgaagacc
    16501 agaaaaaaat cattcataag gatatgaatg tgggggccag gccattgatt atcaactacc
    16561 cgtgttgtca tcgacgggcg aactccataa gatgtgcagc attcacgttc ggacggagtt
    16621 cctgacctat gtcccctact atttatgata ttgccagggt tgcaggcgta tcaaaatcca
    16681 ccgtatcacg cgtgctgaat aagcaaacca atatctcccc ggaagcgcgc gaaaaagtgt
    16741 tacgggccat tgaagaatta cagtatcaac caaacaagct ggcccgcgcg ctgacctctt
    16801 cgggttttga tgccattatg gtgatttcta cccgttcgac caaaactacg gcgggtaatc
    16861 cgtttttctc tgaagttttg catgccatca ccgccaaagc tgaagaagaa ggtttcgacg
    16921 tgatattgca gacgtcgcac aacccggcag aagatctgca aaaatgtgaa agtaaaatta
    16981 agcagaaaat gattaaaggc attattatgc taagctcacc tgctgatgaa tcgttttttg
    17041 cgcaactcga taaatacgat attccggtgg tggtgattgg caaagttgaa ggtcaatatg
    17101 cccatgttta ttctgtcgat accgataatt ttggcgacag cattgcgttg accgatgcgt
    17161 taattgaaag tggacatcaa aatattgcct gtctgcatgc accgcttgat gtccatgttt
    17221 cagtggatcg ggtaaatggt tataagcaga gtctggaaga tcataatatt gcagtgcgtg
    17281 atgaatggat tgttgatggc ggttataccc atgaaacagc actgcaagca gcacggcaat
    17341 tattaagtca gtcaccgttg cctgaggcag tgtttgccac tgacagcctg aaattaatga
    17401 gcatttatcg tgcagcggca gagaaaaaca ttgctattcc gcatcagtta gcggtggtgg
    17461 gttatagtaa tgaaacgctg tcgtttatat taacgcctgc accgggcggc atcgatgttc
    17521 cgacgcagga gttaggtcaa caaagctgcg agttattatt ccgcttaatt tctggaaaac
    17581 cgtcaccaca gaatattacc gttgccacgc atatgtcgtt gaaataaatg tatgccggat
    17641 ttggcgctcg cgccgcatcc ggcaatttag cccattatca gaacgagtaa tttacgccga
    17701 cacctgcata atggaattta tcactgtctc cttcgtcgtg atcctgccac tcaaacgcgt
    17761 attccagcga aacggaaaga ccattctgga aatcataacc gtaaaataaa ccaacacggt
    17821 taaaatcatg gccttcacgt tcaatatcat cctgccagtc ccagttactc cagcgatcca
    17881 gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc aaataggcgc
    17941 gaatttcttg cgtggaaaac tcaccgttat tgcggctgtc gtccatattg aagccgcgct
    18001 cgagataata gttcactcgc aaggcaaccg tttcgttgaa ggtatattgc agacctgttt
    18061 ccgtttcgac acgggtatca gcgtaaccgg tcgtgttcag atcgttggca aatttataca
    18121 tcgacaacca accgttgaaa cgtaaatcgt cagtcagttt cacatcccag tctggcgcga
    18181 ttttccagcg ctgcatattc gccgtatctt tacccggttc atcaacgtag tgataaccat
    18241 aattacggaa accgccggtc aggccgaaac tgaaatcatc gctttcgagg aactgataat
    18301 gcacctccag ctccgggcga tcaaaccacg ttccacgttt acccgcgcta taatctaccg
    18361 gcccttcctg ataataggcc agagcaattc tccacggccc gttggcggca ttaaaataga
    18421 ctgaaggctc cgccagccca tccatatctt cgccataacc ctcgacgttt tctatttcgt
    18481 acatcgcgcc gatattaaag tgccagtcgt tcttttcctc ggcctgtgcg caggccattc
    18541 ccgcacacat caccagtgcg gtacagggta ataacttttt cattattgtt ccttgtatta
    18601 ttctggggga atgccttccc cctgtattta gcgaattgct atttccgttt ctgcatcaaa
    18661 gaaatgacat ttcgtcatat caaaatgaat agtgatattt tctcctgcat gataatcatt
    18721 taacgcacca gcacggacca ctaactcgtg ccccccaacc gtggtgtaga gcataaattc
    18781 cgcaccggtt aattctgcca cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc
    18841 cggatgaata tcttccggac gtattcccat cacgatgggc ttatgcaaac ttccctgtgt
    18901 tttcagaacc gctaatttct cttcgggaat ggttaattta agcgtttccg taacgaattt
    18961 atcgccatcg atcgtgccac gaataaaatt catcgccggt gatccaataa atccggcaac
    19021 aaacatattc gctggttggt tataaaccgt tttcggcgca ccaacttgct gaacaatccc
    19081 atccttcata atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat cgtgggtcac
    19141 gtagatcatg gtggtattca gcttctggtg cagcttgctg atctctgcgc gcatttgcac
    19201 gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg
    19261 tacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt
    19321 gcgtttcagg tactcacgca ggccgagaat ttgcgcagcc cagttaacgc gctcatcaat
    19381 cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt agaccgtcat
    19441 atgcggatac agcgcatagt tctggaacac catcgcgata ttgcgctctt tagccggaac
    19501 gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc
    19561 aatcatgcgc agcgtagtcg atttaccgca gcctgacgga ccgacaaata cgatgaactc
    19621 tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt
    19681 ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga
    19741 aggccgataa ccgcgaccag gtcaggaagt ccgttgaggg caacagtaat tgcgccccgg
    19801 ttaagcccgc gccgatcccc accgagcgca taccactggc gttaatggcg tcaatgcccg
    19861 cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag
    19921 cgagaaagat ttccgggtcc ggtttcgagt ttttaagttg ggaagcgtcc gcgcagaagg
    19981 tgaaaaactc acgtagctcc aacgccgcta aaatcgtcgg cgcattcagg gagacagaag
    20041 ccagccctat agggatctgc tgtgcgcaga gatctgccag caaagagcga atgccgggca
    20101 acacagcatt aaccgtcagt tcgcgcagtg agtggacgta gagcagattt ttgcgatacg
    20161 ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gtccccgtgt tgcagaatgc
    20221 gccgcagaga ctcatcgcgg ctgatccctt ttagggattc gttaaactgc gcatcaatgc
    20281 tgatgccaat ttcagcagca atctgctgcc acgccttgaa atgcagatgc gcggtatcgg
    20341 tgattacacc atccaggtcg aaaactactc cttgcagttt cattcatcct cccgatgcgt
    20401 ggtagcggtc ccattgaagg gcaaaataaa atcgcccaaa cagaaaacag attcttcagc
    20461 cacggatata agctggccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat
    20521 acgctgcgcg tcgagcgtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa
    20581 ctgttgccac tgctcaggta acgccggatt gagatgcaat tcaccgtcac gcacgctcaa
    20641 cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag cgtggatacc
    20701 atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg
    20761 ataactttgg gtcagcaggc cacagcgtgc ggcaacaatg ccgtggattg ctttacttaa
    20821 tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccgc
    20881 tgagaactgc tccggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg
    20941 catctcgttc acttctgcgc gtgaataatc cagcagaatg gtttgctttc ccgccgccgc
    21001 tttgtatttc gccaggttaa tcgccggctt agccataaac gaatcatcct gcggcaaaac
    21061 gccgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg cgcgatggat
    21121 aaacgcatcg tcgctacagc caaattgacg agcgatgttc agcgcctgct ggacgttgta
    21181 atacgccata tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc
    21241 aataacatca tgaatttcca gacgatcgtt aactctcacc gcgcggctaa tccagaactt
    21301 tgccgtctcc agaagtagcg ccatgccttc atgagcaatg aaactttcat cccctgtggt
    21361 ctgccagtat tgaataaccg cccaggcgat atcggcgacc agatgatgtt ccgcctgcgc
    21421 cgaagccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc
    21481 ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg
    21541 cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac
    21601 cgtcggatcg ctaaacagat gaaacggtag caaaaatact tctgtatccc agaaaacgtg
    21661 gcctttgtag ccttctccgg ttaagccttt tgccgcaata ctgctgcgct cgtcgtgagc
    21721 aggcgtcatg atgcgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc
    21781 gccgccgttt accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc
    21841 tgcaagaagt tggtcgtaac tctgctgcgc gcacatttca agcaggcaaa gcgacgcgct
    21901 gccccactcg tctaaaacgg cttgcctgtc atcccgccag tcgatccaca ccagtttttg
    21961 caacgtcact gtctcgcctg catgaagctg cgcactggta tgttgcagca aacggcgctc
    22021 tttagccgta tagcattgct gaacgtcacc actcacctgg cagcaacagc taattgccac
    22081 atcactgcgt ccatcctgag tggtatagat tccttgcatc aaatgctgtc caaacactcg
    22141 cacctgggtt tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt
    22201 tgaaatcagc actgacgcgt cggcgtccag tggagtaata gtgatttcca gcgcaatgag
    22261 cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg tgccgttgct
    22321 ggtacgccag acaacgctgc ggcgtaattc gccactggca aagtcaagct cacgctgcca
    22381 ggcttcgcgg gataatgaga aaatctcacc atttatggca atctccatcc ccaccacatc
    22441 gggcaggttc accagttcgt tgatttcacc tttacccgcc cgatgataca gccccgccag
    22501 atacatgccg cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata
    22561 gccgttcccc tgcgccatca gtgatgcata cttgttcagg gtatgctggc tgaaatgggg
    22621 ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga
    22681 tacaacgccg ccaccagttg ctggatgatg tacccctgct cggcatcggc tatcatcacg
    22741 ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct
    22801 gccatttccc gttgcatcag cgtcattaac tcaccgttgt tatcggtgta gatatgtgct
    22861 ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat cgactgttcg
    22921 cggatgttga gcgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg
    22981 gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca
    23041 cagctctttt gcgtgccgat cttttgaaag ctatgcgcat tcacgctttt caccgccgga
    23101 aagcccagca catacatcgc cgcatccagc atatgaatac cgatgtcgat cagcgggcca
    23161 ccgccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
    23221 gcgcgggcgg tggtgacgta aatttcgccc aggatgccgt tagtcacttg ctcacgcagt
    23281 tgttgtgtat caagcgcaaa acggtgatgg aagtcgtagg ccagtacttt tccttgtctg
    23341 cgcgcggtat cgcgcatgac ccgagcctgt tccggcgtca tagcaggcgg cttttcgcac
    23401 atcacatggc agccggcttc cagtgccata agggtatgtt catagtgaaa acggttaggt
    23461 gagcagacgc taaccacgtc agggttcacc gccagcagca tggcctgcgg atcgtcccac
    23521 acggatgcat taccgtattt atctgccagc gcctgcgcct gggaaaggcg gctgtcacag
    23581 acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac tttatccgcc
    23641 acctggcccg cgcctatgat cgcgacccgc agcggagagc ttgtcattgc acttttcaca
    23701 aagacctctt agcaggtacg caaccaggcc aaagaatcac ggtacgcctg ggcaggatct
    23761 tctgcccgaa tgcgcccttc atacaccaca tagccctgat aattatcggc gcgcagttgt
    23821 tcaaacagcg cgtggaaatc cagggtgccg ctgcccggct gataacgatg gttatcggca
    23881 atatgcacat gaccgagcag gtcgcggttg tcatgcagcg cctgcgccag gttatcttct
    23941 tcaatattca tgtgatagaa atcgccgata atctgcacat gtttaagatc gttttcgacg
    24001 atgtaacggc gggcatcggc gagagtgttg atcatgtgat cctgataacg gtttaacggt
    24061 tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca
    24121 ctcaccattt tacgatcgcc gtccaggctg cgcggcgagg tcatcggcgg taagcggaag
    24181 gtaaacatgc cccacgctgc cggaacgacg atacctttac cgcccacttc tgccagcact
    24241 tcgagaatgc gctctatctg ctttaagcca ttaagacgac gctcttcgat aaagtcacca
    24301 atccagccgt catagccacc gcaggcggtg gtcaccggta aaccggtttc tttgattgcc
    24361 gctttgactt cttcgatgtt gttgaccagc agtttgccgt caatctcaaa accatcgaag
    24421 cccatctctt tgatataacg aaatttctcc agaatatttt ccgggaagaa cgcctgattc
    24481 tgtgtgccta ttttcatttt ttcattcctt tgaattaaaa cgtgacgccc attttgatgc
    24541 tctgttccgg atgctggtcg acatactgca tgtagctttc cgggctgtta gcaaaggtca
    24601 ccaccgggtc gattaaatcc tcacagttga gataaccgtt catcagcagc tcccagcagg
    24661 tttcttcgat acgcttgcgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc
    24721 gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcgg
    24781 caaacggttt agcaaacgcg acataggaga tggtgccacc gtaagccagg ccgcgcagcg
    24841 ccgactgcag cgcgtcggcg taaccgctgg tttcgataat cacatcggca ccctgcttgc
    24901 cggtcagcgt tttgatctct ttgcctacgt cagtgccaat gggattaagg cagaaatccg
    24961 caccgtgacg acgagcaata tcacaacgat gggcaatagg atcgacgcca attaccaccg
    25021 acgcgccagc gcgtttagcc agttggatgg caatttgacc gatcgcgcca agccctacca
    25081 ccactacaaa atcccctacg cgtacgttgg catcgcgaac gccactcatg gcgaactgcg
    25141 ccgggtcgta gcagacggcg tttttccagg agctgccttc cggcattttg cgcaacttgt
    25201 agttattcac tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt
    25261 cgcccaccgc atagtcggta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt
    25321 tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc acctgccgga cgcggggtga
    25381 acatctgcca ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga
    25441 ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta
    25501 aaatcgcccg atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact
    25561 ttttcatgta aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg
    25621 tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg
    25681 tcaccagcga ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt
    25741 tcaggcccac tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat
    25801 agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca
    25861 ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt
    25921 cttcgatctc atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca
    25981 gagtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaatg ccaagtgcgg
    26041 tgataatttt gaacagcggc accaccagca aaatgccgga gaacatgtac accgtgtaaa
    26101 agctggcgtt gatggtcatc cgacctttaa agcgcaggcg agaaagcgcg taagccccga
    26161 gaataccgag aaataccgcc accacggatg aaaccaccga caccaccatg ctgttacgga
    26221 agtagtcgac aaacgggaaa atcaccgggt taaaaatgtc gacgtaatgc tccagcgtcc
    26281 actgctgtgg cagcagcgta ggatgcagcg agatcgcctc ttttgcgctc ttgaacgagg
    26341 tcatcagcat cacaaaaaac ggaaatagcg tgatgatgag aaacagcgcc agcccgcagt
    26401 aaaaaccgat gcgactgagt gtgcgtttat ttgttgccat tgaggttcac ccgttttctg
    26461 gtcagcagaa tgacagcgaa aataatgatg aatagcacca ccgagatcgc cgccgctttc
    26521 cctaagtcat taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg
    26581 gttgtcagca gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc
    26641 accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca
    26701 ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc
    26761 agaaacgaga taaaggcata tgggaaatag cgccagatgg cgaacagcac caccagcaca
    26821 aagctactgc ccgggttatc gaaccacagc ggtgcctgct cgtagagatg cagaagatcg
    26881 acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcgaacacc
    26941 agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg
    27001 cggaactcgc ggttgaaaaa catcgccacc gccagcccga gcacggtgct gcccgccacc
    27061 accagcgcgg tataccagac cgtcatccac agcgaatgcc agaagccggg atcggagagg
    27121 atacgcacat agttgctcac cccaacaaac gttgactcga tgttgggatt gagcggcaga
    27181 cgtaaaaaac tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt
    27241 aataagctgg gcgcgagaag cagcagcgca aagggcatat cggaacgacc tgaaaaaagc
    27301 ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca tccagttttt
    27361 tctggctcgc ttgcagcgta gtagagagat cggctttacc cacggtgacg ttatgcacca
    27421 ttgaactcac cacgccagaa cccgtcacat cacccatgcg ggtaaagttt ttatccccta
    27481 ctgcgccaaa aacctgaata tttggcagtt caccaattaa ctgattcggc agttcaccca
    27541 gcgccttgat cacgtcgttg tctttccagg tggcggtagt gacaaccgct ttgttcaccg
    27601 gcagcgccgc gcctggcgac atcatcaccc agtcggcaat gttgtctgcc tgctccataa
    27661 aggtgacaaa tttttctgct gcttcggtct cttcggtctt ttgtccggcg gtaatggtca
    27721 gcgaggtcaa catgccgtag accgcagagt ttttctcggt tggcaccacg aaaccgacgt
    27781 ttttcgggtc gccttcttta attacagccg gaaggatata ggtggagtaa atcgccatcg
    27841 gcgcggtgcc gttcataaag gcgtctttca cttccatgat gtcgttagaa cccggcatgg
    27901 tgttggcagc aaggtcgcgg taataggtca gcgcctgcat catctctggt gtatcaaggg
    27961 tgattttgcc ttcggcgtta aagacgttgg cctggttgga taacgcaaac tgggagaagg
    28021 attgttccgt cagcacgctt tctgccgtag gcagcgcaat accgtatttt ttattcgccg
    28081 ggtcattcag tttctgtgca acgtccagca gctgttgcca gtttttcggc tcctccagcc
    28141 cggcttttgc cagcacatct ttgcgatacc agatgccgcc aatccaggcg ctgacgggaa
    28201 caccggtcca ggcactccca tcttcggtgc gcacaatacg cagtacgcca tcgtaaaacg
    28261 cgccttcacc aacgttgctg atgaccgtgg caaccgcctt gcgatcgata agctgctctt
    28321 tgtccataac tttggcgtag tcatggctgg tttcgatcac ttccggcagc gagccgctac
    28381 gtgaaagggt gatgactttg gtgttataag cgtcttcttc cacgggcact tgtttaacgg
    28441 tgatacctgg gttttctttt tcaaaacggg caatcaattt gctgataact gcctgacgct
    28501 cctgctccac cgaagaatgc ataaattcga tggatacatg ggttttattt tcttctttac
    28561 agcctgaaat cagggcgcag gaaaccagtg ctgataacag cacgattttt gatttaatca
    28621 tgagatgttc ctttttattt aatccacata acctgccagg ggcgcagcgt tattttatta
    28681 cccaatatat caacttcact aatcagatcc cgaccgtgca ggccagtaat atttacatgc
    28741 tgaatatttt tactgacatt aaacaatccg gtcagacaat taccctctgc gttacttctt
    28801 tgaatacgca ttacggatga attaaccgta tcgatggtaa aattattatc cggatgaaac
    28861 tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact catgatatac cgcatgcctt
    28921 aatgtagctt catcgttcag ttctcgggtt atctcttcac tgtgatattt tttacggtta
    28981 atcgcacggt tatatccgag tttttcgaca cctgcgtaat cattacgcga gcccagaata
    29041 ctttgaatat atatcgctgg cacaccgggg aaacttaaca aaatcgcatg ggcaaggata
    29101 aacctggcgc aacgttcttc atcgctactc tcgcggcggc ttaacgcatc catataggtg
    29161 acatttattt catagggact gcgcgtaccg tcgggattat ttttccagtt tactaatgca
    29221 ccttcctgct gtaatgcctc gaccagcgct aatatttcgc tttctggtaa caagccccgc
    29281 agtgggttca gcccgatgcc atcgtgagag gcgaggaagt taaaccaggt cgttttaccg
    29341 gagggcaagc tcagactttg cgcccactgg cacagcgcct cgacgttctg tttttgcacc
    29401 gcatgcagca ccagcggcgg cagcgagaac tgatacacca tatgggcttc atcatcgccg
    29461 ttgccaaagt aagcaatgtt gtctttatgc ggaacattgg tctcggtaat gatcactgtg
    29521 cctggcgcga cgttatcaat aatcgaccgt aacagtttga taatcagatg tgttttttcc
    29581 agatggatgc agcttgttcc cggctctttc cacataaagc caacggcatc cagacggaca
    29641 tactctgcgc ccttttccag gtagcacagc aatacatcca ccatcgccag caacacttca
    29701 gggctacggt aattgaggtc aatttgatcg tcgctaaagg tggtccataa atggcgcgtg
    29761 gaattatcat ccatttgaaa cggcgttaat agcggtaacg cacgcggacg ggtgacggca
    29821 ctgagatcgg tttgcggatc aaccgcaata aagaaatctt caaaacccgg aagctgttgc
    29881 aaatagtttt taaaccattc actttttgcc gacatatggt tgcagacaaa atcaaacatt
    29941 aaatgactgc attcaccgag ctgctgaata tcctgccact cccccgcttc actggcgacc
    30001 tgatgataat caattaccga aaatccatca tcagatgacc atggataaaa aggtaataaa
    30061 tgaacatgtg aaaaaatgct ttgcagccat tgatggtaaa actgattaaa tgtgggtagt
    30121 ggttttaaat cattactgtg aaattgatcg gcataggtaa ttaacacgac gtcactttca
    30181 tcccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca
    30241 agtttctgta aatgagttga ggtaaatgtt ccaccgtaaa tttctgccaa gtaatccgta
    30301 atttcctgtt tcatgtgcct ttccgtggta gcggtcccat gaaacgagag actactccgc
    30361 agaaaataaa ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc attttttacc
    30421 agggaaaaag cgtatttgtg agctgacgca aataatctat agggatgtaa ataaggtgtg
    30481 acagaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg
    30541 gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa
    30601 ggatctcttt tgcctgcccg gactggcgtc cggcattgca atacactttc acggtgtcgt
    30661 ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg
    30721 ccccctgaac gtgctcctgc tgatactgtt ctggaacacg aacatcgatc cagtgttcag
    30781 cggcgaaaac gggcagtgaa aacaccagtg tcagagcaag taagcctttt ttaaacatgt
    30841 aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt
    30901 gcgaaactcc cctcaccttt tataacgctg cgccagttta ttggcagccc gacttaacag
    30961 cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc
    31021 ccagcccgcc acacccgctg ggccatagcg cagtgcggta agaagtacca gcttgcctgc
    31081 taatttgaaa ccaggcttta ccttttgccc ggcctgttgc cagcgagtat tcataacttt
    31141 cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc
    31201 atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg
    31261 ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat
    31321 gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc
    31381 accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc ggcgcagacg
    31441 ccacggacca tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc
    31501 attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac
    31561 gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg
    31621 acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa
    31681 acagcacaaa aatggttaat ggaatagcca gaaatagcgc gctcataagt actccttaga
    31741 cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat
    31801 tttggctttt aattgtgcca gttgctcgct gattgcatca tcggctttca gttcggcaaa
    31861 ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg
    31921 gtcaattcga cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc
    31981 cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg
    32041 ctggcgagca cgggtttcgc tcaatttgtt ttccagctcg ccgatctctt tcttcatacg
    32101 caccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca gatcggtcag
    32161 tttctgtttt tcaattaacg ccgcacgcgc cagatcttct ctctctttca gtagtgccag
    32221 ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag
    32281 ctgtttcttt tccgccagcg cacgcgccga agtagaacgt acttcaacca atgtatcttc
    32341 catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct ctaacagagc
    32401 gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tattgtcctc
    32461 ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt
    32521 ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat
    32581 tagcgaatta cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact
    32641 cggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga
    32701 caaaccggtg ctcatcatcg gcgagcgcgg cacaggtaaa gaactgatcg ccagccgtct
    32761 gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa
    32821 tgaaaatctg ctggattccg aactgtttgg tcacgaagct ggcgcgttta ccggtgcgca
    32881 aaaacgtcat ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact
    32941 cgctacggca ccgatgatgg tgcaggaaaa attattgcgc gtgattgagt acggtgaact
    33001 ggagcgcgta ggcggtagtc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa
    33061 tgccgatctc ccggcgatgg tcaatgaagg cacttttcgc gcagacctgc tcgaccgact
    33121 ggcttttgat gttgtacaac tgccaccact gcgcgagcgc gaaagcgaca ttatgttgat
    33181 ggcagaacac tttgccatcc agatgtgtcg ggaaatcaag ctgcctctgt tcccgggttt
    33241 taccgagcac gccagagaaa cattgctgaa ttatcgctgg ccgggaaata ttcgtgaatt
    33301 gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga
    33361 catcattctt gatcccttta aacggcgtcc accagaagaa gccaacgccg tttcagaaaa
    33421 cacttcgctt ccgatgctgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga
    33481 gttgctgcaa cacagtttgc aacaagggaa atataaccag aagcgcgcag ctgaattgct
    33541 ggggttaacc tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg
    33601 ggcaatacaa cttgccggat gcggcgtgaa tgctttatcc ggcatacata atcaatacgc
    33661 cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct
    33721 cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg
    33781 atacaggaaa cgaatcacgt tgccgtatgc gccacaggtc agcagaagca gcccctgcgc
    33841 cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc
    33901 gttaaactct gccgcaatca tagaccccag accgcgtacc gccgcgatgg ccggaacgct
    33961 ttctttggca tcaatcagcg tgtttgtcag acgctggccc agttgattcg cgcgttcgca
    34021 gagtgattct ttgtcgataa tgttgagcac cgcgtgcgcg gcagccaccg ccagcgggtt
    34081 accggcgtag gtgccgccaa gcccgcccgg cgcgggtgcg tccataatat tcgcgttacc
    34141 gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg
    34201 cttatcggcg taatgatcca tcgcaaacag tttgccggta cgcgcaaagc cgctttgcac
    34261 ttcatcggca atcatcacaa taccgtgttc gtcgcacagg cggcgaatgg cggcaaccaa
    34321 ttcttttggc gccacgttga aaccgccctc gccctgaacc ggttcaaaaa taatcgccgc
    34381 gacctgtttc gcttcgatgt ctgatttaaa caaacgttcg atggcgtcca gggagtcctg
    34441 cgttgaaacg tcatgtaaat ctgacggata cggcacgtga tacaccgaac cggggaacgg
    34501 gccgaagccg attttgtacg gcgcgacctt tccggtcagc gccatggtca tgtacgtacg
    34561 accgtggaag ccgccgctaa acgcaatcac acctgggcgt ccggtatggg cgcgggcgat
    34621 tttcaccgcg ttctcaaccg cttccgcacc ggtggtgaag aacgcggttt tagcctgccc
    34681 gcttaccggg gcaagggcgt tgattttctc cgccagggtg acgtagcttt catacggcac
    34741 aatctgatac gcggtgtggg taaactgttg cagttgctgc tccaccgccg cgaccagatc
    34801 agggtggcga tgtccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt
    34861 gccctcaaca tccttcagcg tagcgttttc agccgactgg gcgaagaagt tacacatcac
    34921 gccaaccccg cgcggggtgg cagaaagacg acgctgatgg aattcattgt tgctcatgtt
    34981 tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc
    35041 tgtaataagc cgcgcccatc gcggtaaacg gcacacgcag cgtgcgcccg ccggggaacg
    35101 ggtaatgcgg cagattggcg aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg
    35161 caatcaaacg tccggcaaga tgagtacagg tgacgccgtg gccgctgtaa ccctgcatgt
    35221 aatagatgtt ggtatcgagg cgaccgaact gcggcatacg cgacagggtc agcaggaagt
    35281 tgcccgtcca gcgatagtcg attttcacgc ccttcaactg cgggaaggtt ttcagcagtt
    35341 tcggcaccac aagacgctca acgtcatccg ggtcgcgcgc gccgtacacc acgccgccgc
    35401 cgtacagcaa acggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt
    35461 ccacacagta gtttttcggg atcagcgaac gggctaaatc ttctgacagc cgctcagtgg
    35521 tgatcacctg cgtgccgcac ggcatactac gcttcgccag ttccggctcc actttatcgc
    35581 ccaggtacgc attcccggcg acaatcacat acttcgccgt cacctggcct ttggtagtac
    35641 gcaccacggc gggcgtggtg tgctggatct gcgtcaccgc agaaagctca tacacgcgcc
    35701 cgccgttcag gcggatagcg tccgcttcac caatggcgag gttcagcggg tgaatatgcc
    35761 cgccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatgg
    35821 cgttggcgtc cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct
    35881 cttcgagtgt ggcgagctgt ttatcattca tcgccacaaa caggccgccg gggcggtagt
    35941 cgcagtcaat ctgatagcgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca
    36001 tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc
    36061 ggctgtagga gttcacaagc tgccccccgt tgcgtccgct ggcgccaaag ccgatgcgtg
    36121 aggcttcgag aaccactacg tcaaagcccg cttccgccag atgtagcgcg gaagacagcc
    36181 cggtatagcc gccgccgacc acgcatacgt cgcaggtgat tgactcattc agtgtgtcga
    36241 atggcgcgta tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag
    36301 gcctccaggc ttatccagat ggttttcagt tcggtgaatt tttcaagggc atgcagggat
    36361 ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg
    36421 ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg
    36481 gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt
    36541 agcgcctgtt cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg
    36601 cggcttaagg acgcattcgg gtctaaatcc acaaagatgg tcgggccgat ggcggcagcc
    36661 agcccggcgt tacggccatc caacaacagt tgccctttgc tttcgccttc ccgaataaag
    36721 ctatggaccg agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca
    36781 agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg
    36841 gcgatgctct cttccagcaa caggcgcgtt ccggcgatac acacctgccc ctggttgtag
    36901 aaaatgcccg cagcggtcgc actcgccgct ttttgcagat ccgggcagtc agcgaaaacg
    36961 atgttggcgc ttttgccacc tgcttccagc cagacgcgtt tcatgttgct gtcgcccgca
    37021 tctttcagca gctgtttccc ggtacgggtc gaaccggtaa aggcgatggc atcgatatcg
    37081 ttatggcgcg acagcgcctg ccccgcttca tgaccaaaac ccgttactac gttcaacaca
    37141 ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat
    37201 ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc aagcttccag
    37261 caagtcagca gcagcgggaa gttccacggt acaatggcgg caattacgcc gaccggttca
    37321 cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg
    37381 atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc tcgcagactg
    37441 tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgttggct
    37501 tccattaaat cggcgagttt attcagcacc gctttacgtt tagccggaga agagagtgac
    37561 cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc
    37621 ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt
    37681 tcattttccg ccgcagcagt atattcaccg ttaataaata agcgggtttc aatggcgaga
    37741 cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca
    37801 cgactatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc
    37861 ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta
    37921 cagaacaaaa agattaaaac gtggtgggcg tatgggcgct aataattcgg caaatacctg
    37981 ccgatgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga
    38041 cgagatggta atcctgacca ttaatcgtca gaacaatttc accttccagt acagtgccta
    38101 tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa
    38161 agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct
    38221 gactgcccat ctcaattaaa tcgtcctgat taatgacgac ctgcggctca tcaggttttt
    38281 ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg
    38341 tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc
    38401 cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc
    38461 gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca
    38521 gagtcgctgt ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat
    38581 acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa
    38641 aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg
    38701 caaccgtggg ctaacgacct tcgccccttg tccatgcaga gagtttaccc aaaagttgct
    38761 acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg
    38821 tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg
    38881 ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg
    38941 gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag
    39001 aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg
    39061 cacattgctg ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag
    39121 taaggacggt tccgccagcg catgtggcag cgcaattggc aagccgcctg catggatgat
    39181 ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca
    39241 cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg
    39301 gactaaatta tcgccattac tgcggtttaa atgaccttta tgttcaatat tttttcaatc
    39361 tagcagtggg ttttcatttt tgcaaactca atttaacatt tgacaaacat ttagtttgca
    39421 tacagattcg aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca
    39481 aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga
    39541 agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa
    39601 tacgcaatat gttgatgttt tacttaccga tttgaatggt tgcttccgtg gaaaacgtat
    39661 tcctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc
    39721 aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc
    39781 ggatcgtacc tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt
    39841 tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc
    39901 gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggtcgt
    39961 agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag ggtatctgca
    40021 accgccctgc gcgcccggca ctgatgaccg caatacacaa agccaggttt actccgttga
    40081 taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttacagct
    40141 gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta
    40201 ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg
    40261 tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca
    40321 cgcgggcagc ggaatgcata tccatatcag tatgcaaaat aatcgtggcg agaacgtgct
    40381 ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg gaatgatcga
    40441 tctgatgccg tcatcaatgg cgttgttggc accaaacgtg aactcgtatc gccgcttcca
    40501 gccggggatg tatgtaccga cgcaggcgtc gtggggccat aataaccgta ccgtcgccct
    40561 gcgtattcca tgcggcgatc gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga
    40621 tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcacg gtcttgataa
    40681 cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggatg gcttaccctt
    40741 cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta
    40801 tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt
    40861 tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcat agtacccctt
    40921 gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca
    40981 tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac
    41041 tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg
    41101 tatgcgcccc tcctgcgact gactggactc cgcccccttt gcttcccttg ctaaacaggt
    41161 ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct
    41221 attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg
    41281 aaattgtggc aggtggtgat gatgggtctg gcctatctca cgccgatgac tgtgttcgat
    41341 acctttggca ttgtatctgg cattagcgac ggtcacgtcc cggcatccta tttgctggcg
    41401 ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag
    41461 gccggttcgg cctataccta cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc
    41521 ggctggtcat cactgctgga ttatctcttt ttgccgatga tcaacgtact gttggcaaaa
    41581 atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt gaccttcgtc
    41641 gccattttaa ctgccgcgaa cctgaagagc gttaacctgg tcgccaactt caataccctg
    41701 tttgtgctgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg
    41761 cataaaggag aaggcgttgg caccgtctgg tcacttcaac cgtttatcag cgagaacgcg
    41821 cacctgatcc cgattattac cggggcgacg attgtgtgct tctcgttcct cggtttcgat
    41881 gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc
    41941 ttcctgacgg cggtctacgg tggcgttatc tttatcgcgg cgtcgttctt tatgcagctg
    42001 ttctttcccg atatcagccg ctttaaagac ccggacgctg cactgcctga aattgcgctg
    42061 tacgtcggcg gcaagctgtt ccagtctatt ttcctctgca ccacatttgt gaacacattg
    42121 gcctctggcc tggcttcaca tgccagcgtg tcgcgcctgc tgtatgtaat ggggcgtgac
    42181 aatgtgttcc cagaacgtgt gtttggctat gtacacccga aatggcgtac cccggctctg
    42241 aacgtcatta tggtcggtat tgtcgcgatg tcggcattgt tctttgattt agtcaccgcg
    42301 acagcattga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt
    42361 aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg
    42421 ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct cgagtcaacg
    42481 tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc
    42541 cgccgctatc gcaaagtgcc gttgtatgat ggtgacagaa cgccagtgag cgaaacgtaa
    42601 tagtcggatg cgcggcgcat tatcctgcct gagggtaatg cgccgcgcca ttaactataa
    42661 aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc
    42721 ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta
    42781 cgcgaccgaa tgcgcagagc ctgtcgcaga cgctgaacgc acaccgctgg cccactactt
    42841 ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga
    42901 aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa
    42961 tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtctggat
    43021 aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt
    43081 tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga
    43141 aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg
    43201 gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag
    43261 atagcttctt tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt
    43321 gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga
    43381 tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc
    43441 caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct
    43501 ggatgttgat ccctatacct atcgcctgat gccagaactt gccgaaagct gggaagtact
    43561 cgacaacggc gcgacctatc gcttccacct acgtcgcgat gttccgtttc aaaaaaccga
    43621 ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat
    43681 ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag
    43741 cctgcaattt gccgataacg tgaaaagcgt ccgtaaactg gataatcata ccgttgagtt
    43801 ccgtctggct cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt
    43861 catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg
    43921 tcaaccggtc ggcactggac cgtatcagtt gtcggaatac cgcgccgggc aatttattcg
    43981 cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg tggtggtgga
    44041 tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct
    44101 ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac
    44161 gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa
    44221 taatcccgct gtccgccatg cgctggcact ggcgattaat aaccagcgcc tgatgcaatc
    44281 catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta
    44341 tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaagtc
    44401 gttggggctg gaaaatttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa
    44461 ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa
    44521 agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca
    44581 tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc
    44641 gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa
    44701 attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc
    44761 ctatgacgaa gcgcagagta ttctggcgca ggaattgccc attttgccgc tggcgtcgtc
    44821 attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa
    44881 cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc
    44941 ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc
    45001 ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc
    45061 tgggtgttct ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt
    45121 caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc
    45181 gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc
    45241 cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg
    45301 ttctggctgg cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt
    45361 tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat
    45421 gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg
    45481 atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt
    45541 atcagcacta ttgaagtgta tgaccagaac tatgttaaag cggcggcgac ccgcggtttg
    45601 tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct
    45661 cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt
    45721 agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc
    45781 atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat
    45841 attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt
    45901 atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga
    45961 cgcctctgcg atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg
    46021 cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttaccaat tattgccgcc
    46081 gtcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga
    46141 tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac
    46201 gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct
    46261 ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt ccctgctgtt
    46321 ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg
    46381 gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga
    46441 aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt
    46501 tgctgtgatg cctaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat
    46561 ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc
    46621 tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt
    46681 catgctgcca ggtgcggcaa ttatgattag cgtgctgttg gttaacctcc ttggcgacgg
    46741 tgtccgtcgt gcaattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg
    46801 actattgaat ttaaaaccgg ggatgagtgg gttaaagccg tcgaccgggt aagtatgacc
    46861 ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt
    46921 gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt
    46981 tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat
    47041 aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt
    47101 cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt
    47161 ggctggcgca aacggcgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa
    47221 gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata
    47281 gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg
    47341 gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctaaacca aaacagcaat
    47401 accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt
    47461 aacgtgcttt actgcggtca aacggtggaa accgcgccga gtaaggagtt agtgacgatg
    47521 ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg
    47581 ccgcataaaa gtcgcctcaa tacgctaccc ggcgctatcc cactgctgga acagttaccg
    47641 attggttgtc gtctggggcc acgttgccca tatgcacaac gagaatgcat tgtgacgcca
    47701 cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa
    47761 gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg
    47821 gaccggttgg tttcgtcgcc agaccgtaga agcggtaaaa cccttgagct ttacgctacg
    47881 tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa
    47941 aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccact
    48001 gcattttggc gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc
    48061 gacctcgttg aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgcctgaa
    48121 caccaacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg
    48181 gctgctaccg gatcacgtca gttactatcc gcatatgttg gcccccgggc aaaaacagcg
    48241 tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct
    48301 cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg aattacagga
    48361 aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag
    48421 cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt
    48481 gctggcatca ccgctgcatg aactcaccaa acggctgatt gccggtcatt ttggcgaggc
    48541 attgacggcg gatgcgtggc gtaaggaccg ttagtgttct gcacctcatc ctgacagggg
    48601 ccccccctca ggatgaggta ttcattactt ttgattgtcg attctggtga gagcaaccag
    48661 gaatgccagt gtataacaca ccgtaattgc cagactcatt tttaacagtg tttctccgcc
    48721 aaatcccgac aatggcgcgg ctataccgcc gaagacaaac atgctcatcc ccatcaacgc
    48781 agacgccgtt ccagattcct gtgtccctac tgcactcata gcctctgccc cgccaacagt
    48841 gccgatgccg ctacaaaatg caatagtgaa aaataatgcc accagtgccg gaatgggcaa
    48901 ttgtgtccat gcgcataaga ccgtgagcaa tgcacacaaa attgccgcta tcaggccacc
    48961 tcgcaacaat gtcatcgcat taatccgtcg cgccagacgc gaaaagatcc atgaagcaat
    49021 gataagtccg atgccgttaa ggccaaacac caggctaaat tgcattggac taaagccaaa
    49081 ttccttctgc aacacgaacg acgaagagcc gatatacgca aacaagcctg ccagcataaa
    49141 actttgaatc aggcaaaagc gcataaaacg gcggttctgc accacgcttc gtccggttaa
    49201 tagcaatgat gagccccttt tattttctgg caaggtctca ttaataaata acacgcagcc
    49261 cagcaacaat acggtgctaa tttcagccat tacccagaat aaagtgcgcc agtcaaaagt
    49321 gctaacaatg tacccgccca gcactggcga caacaccggt gccaggccat tcaccgtcat
    49381 taacagcgca aaaaactggg tcaacgttac tccctgatat ttgtcacgag caatagaacg
    49441 agagagcacc gaaccacccg cccccgcgat cccttgaata aaacgccaga ccaccagcca
    49501 gtaaatattg ttcgttgtcg cgcacaaaat ggaagaaaca ataaacaata gcaacgacaa
    49561 gattaacgga cgtttacgcc ctattttgtc gctcagaggg ccaaataaca gttgtccgac
    49621 gccaagaccg atcagtgatg cagtcagcgt taattgcgtt atggtcgtgg ttgccgccag
    49681 ttgctcagac atctccggca aagccggcaa ataaagatcg gtacacatcg ggccgatacc
    49741 ggctaaaaga ccaagaatca atgcccatga aggagagact ctagccatat tattccttac
    49801 ttgagtgatg acatgccgcc gcccagcgac tgccagagcg tgattcggtt tgtaaaatca
    49861 gtcagttgca gtgccagcag ctcctgctgt gcgctccaca agctgcgctg cgccgtcaac
    49921 acggtcaggt aatcaccaac acccgtctga tagcgacgca agccaacatc gaccgtttgt
    49981 tgctcagctt taacatactg gcgctgtgca tccagttgct cttcaagcgt ggtccgccgc
    50041 gccagtgcgt tagcgacatc tttaaatgcg ctctgaacgg ttttttcata ggtggcaatg
    50101 agtccacgct tttgtgcttc tgcgtaacga agctgcgcca gattacttcc gccagtgaac
    50161 agcggtaatg tgaccgatgg cgtaaatgac cagatctgca ttccgtggct gaacaacgac
    50221 gacaatgcgt cactgccaac accagcgctg gcagtcagcg aaatcgtcgg gaaaaagtta
    50281 gcccgcgccg cgccaatatc ggcattggcg ctcttcaaat tatgttccgc ttcctggata
    50341 tccgggcgac gcagcagaac gtcagatgac actcccgcag gaaccagact tatcatctgt
    50401 tccggcaggc tttccagtgt tcccggtagc aaattttcct caagcgttgt acctgccagt
    50461 aagttcagcg catttttatc ctgcatcact tgtgtctgat agctggcaac gctggcgcgc
    50521 gcttgctgat aaacactcat cgcctcactg acatctgtcg ctgccgccgt gccaacctgt
    50581 tgctggcgct ggataatttt caatgagttt tctgcactgg tcatcgtttc ttttgccagg
    50641 gccaggttgc tgttatcggc cgccagtgtc agccaggcgg tgctgatttc agcaatcagc
    50701 gttaaacggg tgttctgggc tgtaaattca ctggcaagcc aggtttctcg cgccgcccga
    50761 gataaactct gattacgacc aaacagatct aacgtaaaac tggacaccgt gccgtcagcc
    50821 tcggcagtgg tttccgtacc attcgccagc gaacgactgc gggttgaact taaggccgca
    50881 ttcaccgtcg ggaataacga cgcattagtt tgcccataga gcgcccgtgc tgagtcaata
    50941 tcagcaatcg ctttttgcac atcgcggtta ctgttcagtg caatagtcac cacctgctgc
    51001 aatcggggat cgtggatcac ctgctgccag tcatggctga ttgcctttcc ctggccctgt
    51061 gctcccggca aggtcgccgg gattggggat tcaggcgtgc tgtagtgggg atcgagcgag
    51121 acacatcccg cactcaacag caccagaaaa ataagagaac gacgcaacat aacttactcc
    51181 tgacggcgcg atttaccggc aaacaaacgt ttcaccagta caaaaaacag aggaacaaag
    51241 aaaatagcca gcaacgtagc ggtcagcgta ccgccaataa tgcccgtacc aatggcgatg
    51301 cggctgttcg cccctgcgcc ggttgctatc gccagcggca ttacccccgc aataaacgcc
    51361 agcgaggtca tgatgattgg gcgtaaacga gtctgcgccg cgcgtaatgc cgcacgactc
    51421 aaggaataac cctctgcaac cgccgcttca gcaaattcca caatcagaat ggcgtttttc
    51481 gacgacaagc cgatagtggt taatagcgcc acctggaagt aaacatcgtt gtttaaatca
    51541 cgcatccagg ccgccagcgc cgcgccaaga agccccagag ggatcaccag aatgaccgag
    51601 aacgggactg accagctttc gtacagtgct gcaaggcaga ggaaaaccac cagaatagaa
    51661 actgcataca gactcaatgc ctgaccgctg gccagtttct cctgcaatga caaaccactc
    51721 caggcccagg ttgttcccgc aggaagactg tttgccagtt cctccatttt tgtcattgca
    51781 tcgccggaac taaagccagt cgcattttcc ccctgaattt cataggctgc agaaccgttg
    51841 tagcgcacca gtctttccgg tccatacagc cagcgggtgg tcgcaaaagc agagaatggt
    51901 gtcatggcgt tatcactacc gcgcacaaac catttaccta agtctgacgg cgcggaacga
    51961 aattcactgt cgccctgaat gtagactttt ttcacacgac cacggtcaat aaagtcattc
    52021 acataagtac cgccccacgc actggacagg gtgtcggtga catcattcaa actcaaccct
    52081 aatgacaccg ctttattact atcaatatct acctgcaatt gcggcatttg tggcaaatca
    52141 ttggcacgaa cagaatgtaa ctccggactt tgattcgctt tttctatcaa ctgattacgc
    52201 atttgcagta gtgtttcgcg atcggttcca ccgttagcta acaattcaaa cgtaaaacca
    52261 ttgctttgcc ccagcccatc aacggctggc ggcgtcatcg caaataccgt ggcatcacga
    52321 attgtgccca gctctttggt tgcccgtagg gcgatagcct gggcggtgtt ttctgcccct
    52381 ttacgttgag accagttttt caacgaaaca aacgccatcc cggtgttctg tccgctgccg
    52441 ctaaaactga aaccatcaac ggtaaaaatg acatcggtat ttgctttctc gttaatcagg
    52501 aaccagtcaa caatctggcg attcacttct gctgtacggg cagcggtcgc cccggccggc
    52561 agcgtgtact gcaccatgat ttcgccctgg tcttcagtgg gtaagaaact gcccggcagt
    52621 ttccacatca tcagggccat tccaccaccc agtacgagat aaagccccat cgttcgagca
    52681 gcacggcgca ggacataaat tacgcctcgc tgatatttat cttcagtacg gcggtaaaag
    52741 cggttaaatg cgccgaaaaa gcctttttta tgtggcggaa catgctggag gacggaaccg
    52801 cacagggcgg gagtcaaggt caatgccacc accacggaaa gcagcattgc ggagataatg
    52861 gttatcgaaa actgacgata aattaccccc gtagatcccc caaagaaggc cataggcagg
    52921 aatacggctg acaacaccag cgcaatggca accagtgcgc cagaaatctc gcccattgat
    52981 ttttccgtgg cttcacgcgc gggcaaccct tcgtcacgca taatgcgttc gacgttctcc
    53041 actaccacaa tggcatcgtc caccagcaag ccgatcgcca gcaccattgc aaacaacgtc
    53101 agggtattaa tcgaatagcc aaacaacgca agaacgccga atgtgcccag caaaacgacg
    53161 gggacagcta acgccggaat cagtgtggca cgcaggtttt gcaggaataa atacatcacg
    53221 caaaccacca atacgatggc ttcaaacagc gtctgaatta cgtcctctac agagatttta
    53281 ataaattcag tgctgtcttt cgggtaagcg atgtcgtatc cctgcggcat gttccgctgg
    53341 aattcggcaa tcttatcctt gaccagcgtc gccgtattca gcgcattcgc accgggcgaa
    53401 agcattacag caaccccggc agacgggtga ccgttaagtt tgccgatagc ggtataatct
    53461 tcactgccca tctcaacgcg agccacatct tttatacgaa caactgcgcc gtctgactgg
    53521 cttttcacga taatattttt gaactgatcc accgtctgca agcgagactg cgcacgtacc
    53581 gttgcggtca gttgctgagt attcggtgaa ggcaatgccc ctattttccc ggcagtaacc
    53641 tgcacgtttt gcgcttcaat agcactttgc acgtctgaag gcattagcga gtaagacgcc
    53701 agtttggccg ggtcaagcca gatgcgcata gcgtattccg cgccaaagac ttgcagactc
    53761 cccacacctt caacacgcgc cagcgggtcc tgaacgttac taaccaacca gtcggcgata
    53821 tccgaactgg aggctttgtc agtggtgtca tacacggcag caatcagcaa aaagttactt
    53881 tgtgatttct ccaccgtaac acccgtttgc tgcacttcgc tgggtaggcg cgactccgcc
    53941 tgctgaattt tattctgcac ctgaacctgt gcagtatctg gatcggtacc ttgttcaaag
    54001 gtcacattaa tactgaccga accatcagag ctgctggttg agctgaaata gagtaaatta
    54061 tcaagcccag tgagttgttg ctcgataacc tgagtcacgc tgttctccag cgtttcggca
    54121 gaagcaccag tataagtggc tgaaatttta atggtcggtg gcgcaacgtc aggatattgc
    54181 gcgacgggca atgtgcgaat agccagaatc cccgccaaca taatcaaaat ggcgataacc
    54241 caggcaaaga ccgggcggcg aacaaaaaag cgcgaaaaca tcaggcgttg cctccattag
    54301 cctgaacttc aacagccttg accgtctgcc ctgaagtgac ttttgcagaa ccttcaacaa
    54361 tcagtcggtc gccgttgtgc aggccgttta gcaccagcca tttatcacca tatgtttctc
    54421 ccgtttcgag cgttcgctgc tctactttat tgtctttatt caccaccagc gcagttgcat
    54481 tgcctttagc atcgcgcgtg acgccctgtt gcggcgcgag aatagcgtct tccatcacgc
    54541 cttcatcgac acgagcgcga acaaacattc ccggtaataa ctgctgttgt ggattgggga
    54601 aaatcgcccg taatgtcacc gaaccggtag actcatctac agcgacttcg gtgagttcca
    54661 gacgcccttt ttcgctgtag gttgtgccat cttccagaat aagtgagacg ctcatggtgt
    54721 cactgttggt cgccagtgac tgtttacgca gacgtaataa atcgacactg gagcgagtga
    54781 ggtcgacata cattgtatcc agaccacgaa tagtcgttaa cgctgtatct tgcgacgcgg
    54841 tcaccagtgc gccaggggtt accgacgaaa tgccaatgcg ccctgaaatc ggtgcggtta
    54901 ccgtggtcca gtcaagatta atgcgcgcag tttccagtgc ggcttttttc gcctctacac
    54961 tggctttatc ttgtgcacag gtagactgag catcatcagc atcctgttgt gaaacaccgt
    55021 tctctttcac cagtcgggca taacgctgcg ctttctggca atcggctttt accagtgcct
    55081 gtgcttgttg taatgctgcc cgagcttcat tccatgcagc ctgataactg gccgcatcaa
    55141 tctggtagag cggctgtcca gccttgacca gatcaccttc cttaaataag cgtttctgga
    55201 taattccccc aacctgcgga cgtacttcgg cactgaacgc agcactggtg cgtccggtta
    55261 attcagtgac taccgagacc ggttgagatt tgactgtaac aacgccgact tctgtttctg
    55321 acggggatga attgttactt tgcgtattat cacaaccaga taagagcagc attgccacta
    55381 cagatgttgc tatatacttc attattttct ccagggtgag aaataccctc ctcattaaaa
    55441 ttaatgagga tatgtcaggg atattcagtt ataacgaacc cgcatcgcga aatgaatttc
    55501 gcaatgaaat aagggggatt aatatccccc gctattggtt aattctgttc ttatttattt
    55561 atcaacatac taacaatatc ctgatatatt tcctgcagat gttgtggatc gcttttttgc
    55621 ggtgttaaac gacgataaac cgtaccttcc atcatggtag caaacacttc aacgcagcaa
    55681 cgaatgtgct cgtcgctaag atggggatgc attcttttca tatgtgcaca accgttagcg
    55741 aacattctgg cgtcagcttc ttcaagcatt gccataaccc gcgggttgcg tgtggcttct
    55801 gcagccactt ccagcatcag cgcgtcgtcg tcctcattta aggatttacg caaggcaagg
    55861 acttccggga agtgatcggt acgcgcgtca atgtccattt gggcaatacg aaagtcgatg
    55921 atacggcgaa ccatctcttc aataatggcg tctttgttgg cgaagtaacg atatatctga
    55981 cccacgctaa gctgagcttc gctggcaatt tgcgacatac tggcggcatg aaacccgcag
    56041 cgacgaaaac accgacgggc ggcgttaatg atttcatcct gacgctgttt gtggcgtatc
    56101 tccagcttgc tggtcatttc tgtctcctgg cctatcactt ggtgagaatg atcgttctca
    56161 tttttgctga ttatacgttt ctccaaagta atggaaagta tttttatctt attcatggtg
    56221 catattctga taatgaagag attgtggaga ttctgtgaag atagttgaca atattaagtt
    56281 ggggtcaatt catcagaaag gctgctactt gttgtcgcca gttctgtttt catcatcaaa
    56341 cttatcggag ctaatacgaa tagtttcata gtcagattct ataagatatt ttcccaacct
    56401 accccttgca ggcgtcaaga gtagataacg caaaatgctc tgtaccgcag cttctctccg
    56461 gtacagaaag cgcaactata gccacccaca gccaggttga ttataataac cgtttatctg
    56521 ttcgtactgt ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa
    56581 taaggattaa agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca
    56641 gcaaactgtc catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg
    56701 cattcaccta ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg
    56761 gttccaatat cgttctgcaa tgcgatgtgg ccgaagatgc cagcatcgac accatgttcg
    56821 ctgaactggg gaaagtttgg ccgaagtttg acggtttcgt acactctatt ggttttgcac
    56881 ctggcgatca gctggatggt gactatgtta acgccgttac tcgtgaaggc ttcaaaattg
    56941 cccacgacat cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga
    57001 atccgggttc tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact
    57061 acaacgttat gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg
    57121 cgatgggtcc ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg
    57181 cggcctccgg tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga
    57241 ttcgccgtac cgttactatt gaagatgtgg gtaactctgc ggcattcctg tgctccgatc
    57301 tctctgccgg tatctccggt gaagtggtcc acgttgacgg cggtttcagc attgctgcaa
    57361 tgaacgaact cgaactgaaa taatcgttct gttggtaaag atgggcggcg ttctgccgcc
    57421 cgttatctct gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc
    57481 cgccttgcgt caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca
    57541 catcaccggc aaaagccact ggtatcatga aacgcaatcc agtactacgg agtatgacgt
    57601 tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc tttctactcg acgtgatcct
    57661 tgaaaaagaa acgctggccc ccttcctttc atggctggac cctgcgcgtg ttcttgcagt
    57721 ggagttgttc cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg
    57781 cttgtcgacg gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta
    57841 ttcggcgcga cttacgccgc tccccgggcc tgattccacc agggaaagta atcatcggtt
    57901 ggcacaaatc acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg
    57961 atcgcgccag catctgaatg acgtcggtct tactgcctgg gactgtgtga tcattaacca
    58021 aatcattggt tttattggct ttcaggcgcg gacaattgcg acatttcagg cttatctcgg
    58081 gcatccggta cgctggttac ccgggctgga gatacaaaac tacgccgacg cgtcactgtt
    58141 tgctgatgaa tcattacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagaaca
    58201 cacaaaaagt tcaactgcag aactttgcca actggccgaa atactctctc tccaccctat
    58261 ttcactttcc cttctcgaaa ggttgttaaa cagcacacgg gttaatacac agccggataa
    58321 tcagcttgcg gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg
    58381 tatggattca tcaaatgaat ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga
    58441 aattaaccaa tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg
    58501 gctgacccga gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga
    58561 aaaatcatca acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa
    58621 tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt
    58681 aaaactgtca gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc
    58741 tgctagcgca gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa
    58801 aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc
    58861 cgccgccaca gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg
    58921 aaaaagaacg tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg
    58981 tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccacttttaa
    59041 aagacgccat tccttgccgc gcagcccgtg gtctgaacca cgagtttaaa gaaggcgact
    59101 gggctgttgc cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgcagaac
    59161 tgacacaata catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc
    59221 gccataatct ggaaaaagaa gcgccagacg gcgtcgctac cgaaatgctc gatgaaggtc
    59281 tggttcgtga agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag
    59341 atatggatga cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg
    59401 cgattgccga tccaaccgcg tggattgctg aaggcagcaa actggacaaa gccgcgaaaa
    59461 ttcgcgcatt caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt
    59521 ctgacgatct ctgctcactg cgcgccaatg aagtccgccc agtactggca tgccgcatga
    59581 cgctctccgc tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat
    59641 ccaaagcgaa gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc
    59701 agcctgaaag tgaagcaatt gccgaacaag tccgtttgct ggcgcaaatt tgccaacgac
    59761 gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta
    59821 ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc
    59881 gtatcgtcga agaagcgatg attgccgcta acatttgtgc ggctcgcgta ctgcgcgata
    59941 agctcggttt tggtatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc
    60001 tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg
    60061 acggtttctg caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc
    60121 gcattcgtcg cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc
    60181 tcggtctgga agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca
    60241 accaccgtct gctgaaagcg gttatcaaag gcgaaactgc gacgcgtcca caggatgaaa
    60301 tcactgtgca aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt gatgttggtg
    60361 actggttata cgcacgcttc ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg
    60421 aaattgtcga tatcagccgt ggcggcatgc gtgttcgttt ggttgataac ggcgctatcg
    60481 cctttattcc tgcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa
    60541 acggcaccgt acaaattaaa ggtgaaacgg tttataaagt aactgacgtt attgacgtca
    60601 ccattgccga agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc
    60661 ctttcacggc ccattcctta tgaatgggcc gtttatttcc ccgctctacc ttcatcatat
    60721 cccggcagtt ttttaactga ctttcgtttg aaaactggcg tttttccaaa actagattac
    60781 aaataaatat ataaaaaaaa caatgtgttt agccgccgtt aatttcatgt aagtcatgtt
    60841 gttcctttta tttaaccgcg gactccgctg ttaaccggag gatatgcatc atgaaaaccg
    60901 ttagggagtc cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga
    60961 cggaaagcag cgatgttttg cgcttttcta ccaccgaaac cacagaacct gaacgtacgt
    61021 tgcagttatc tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc
    61081 tgatgatgag tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa
    61141 tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc
    61201 gtgatttatc acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa
    61261 tagtgattct cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca
    61321 cagggttgaa cgaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc
    61381 gtgaagctgc ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg
    61441 aagtcgaact gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaat
    61501 ttgtccacag cggcagtggc aaaaacgaga tttttttaat ctgttccggc accgacatta
    61561 ccgaagagcg ccgcgctcag gagcgactgc gtattctggc aaataccgac agtatcaccg
    61621 gactgccgaa tcgtaacgca atgcaggagt taatcgatca cgctattaat catgcagata
    61681 acaataaagt tggggttgtg tatcttgatt tggataattt taaaaaggtc aacgacgcct
    61741 atgggcattt gtttggtgac cagttattac gcgacgtgtc attggctatt ttaagctgtc
    61801 tcgaacatga ccaggtgttg gcgcgtccag gtggcgatga gtttctggta ctggcatcca
    61861 acacctcaca aagcgcgctg gaagcaatgg catcacgaat tttgacccgc ttacggctcc
    61921 cctttcgcat tggtttaatt gaagtttata ccagctgttc agtaggtatt gcactctctc
    61981 ccgaacatgg ttcggacagc gcggctatta ttcgtcacgc cgacacagca atgtacacag
    62041 cgaaggaagg cggacgagga caattttgcg tttttactcc agaaatgaat cagcgagtat
    62101 ttgaatatct ctggctggat accaacttgc gtaaagcact ggaaaacgat cagttggtta
    62161 ttcactatca accgaaaatc acctggcgtg gcgaagtgcg cagtctggaa gcactagtac
    62221 gttggcagtc acctgaacgt gggttgattc caccgttgga cttcatttcc tacgcagaag
    62281 agtcagggct aattgtgcct ttaggccgtt gggtgattct cgatgtcgta cgccaggtgg
    62341 cgaagtggcg ggataaaggc attaacctgc gagtggcggt aaacatttct gcacgtcagc
    62401 tcgccgatca aaccattttc accgccctga aacaggttct ccaggaactc aattttgaat
    62461 actgccctat tgatgttgaa ttgacagaga gttgtctgat tgagaatgat gaactggcac
    62521 tgtctgttat tcaacaattt agccaactag gtgcgcaagt gcatctggat gattttggta
    62581 ccggctactc ttcactttcg caactggcgc gctttccgat cgatgccatc aaacttgacc
    62641 aggtttttgt tcgagatatt cacaaacaac ctgtctcgca gtcactggtt cgggcaatcg
    62701 tcgctgtggc ccaggcattg aatcttcagg tgatcgccga aggtgtagag agtgcaaagg
    62761 aagatgcttt tttaaccaag aacgggatca atgagcggca aggatttttg tttgccaaac
    62821 cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta tctgaagcgc gcataagtag
    62881 atgaatatat atacttagcg ctatattccg tcattatata tcgtattccc ggatgataat
    62941 tagccgttgc tgttaaacac ttctctttta gcggcaattt ttgcattatt cattttcccc
    63001 cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta
    63061 ttgatattgt gttagatatc cttgtggcag gcgattacca ctctgcgatc cataatctcg
    63121 agatacttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac atcccgaagg
    63181 caccgtggga gatttaacaa tcgtgccttc aggttttcct atttgaggat gcccggcgcg
    63241 tcagtatttt tttatttagt attataacgt tataagaatt acagcgatga attcccgaca
    63301 acaaactatt ctacagatgg tcattgacca gggtcaggtt agcgtaaccg atctggcaaa
    63361 agccactgga gtttctgaag ttaccattcg ccaggatctc aacaccctcg aaaaactgag
    63421 ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat agtgatgacg tcgaaacccg
    63481 tatgatgagc aactatacgc tgaagcgtga actcgccgag tttgccgcgt cgctggttca
    63541 accgggcgaa accatcttta tcgaaaatgg cagcagcaat gccctgctcg cccggactct
    63601 gggggagcag aagaaaaatg tcactatcat cacggtcagc agctacatcg cgcatttgct
    63661 gaaagacgcg ccttgtgaag ttattttgct cggtggcgtg taccagaaaa aaagcgaaag
    63721 tatggttggc ccgttgacac gccagtgcat ccaacaggta catttcagca aagcatttat
    63781 tggtattgat ggctggcaac ctgaaactgg attcaccggt cgcgatatga tgcgtaccga
    63841 tgtggtgaat gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg acagctcgaa
    63901 atttggtgct gtacattcat actccatcgg tcccgttgag cgattcaatc gcgtgattac
    63961 cgattcgaaa atacgcgcca gcgatctgat gcatcttgag cacagtaaac tcaccgttca
    64021 cgtcgttgac atttaaccat ttcccttctc tacggatgat ttgcagtttg gcaaatcatc
    64081 cgctctaaga tgattcctgg ttgataatta agactattta cctgttatta acactctcaa
    64141 gatataaaat tattatcagc gatataacag gaagtcatta tcacctgcgt gatataaccc
    64201 tgcgcgcgag cggatttcac ggaataattt caccaggctt attcttagct attatagtta
    64261 tagagagctt acttccgtga atcataaatt caggagagag tattatgttt gtaacgagca
    64321 aaaaaatgac cgcggctgtt ctggcaatta ccctggcaat gtctctgagt gcctgttcta
    64381 actggtctaa acgggaccgc aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg
    64441 cagtactgac cgatggcagt acgttgggta cattaggtgg cgcagctgtc ggtggtgtta
    64501 ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc gcaattataa
    64561 tacgcttgat tctgttcagt acgaaataac tgaataatac acacagtaaa atattcgaaa
    64621 gccacggata tatccgtggc tttttatgtt aaccgcctgc gagttttacc ttcatccctt
    64681 tcgcttcaag tagtgatttt aataaatcac gcttatcgcc ctggatttca ataactccat
    64741 ctttaactgc tccgccgcag ccgcattttt tcttcagttc cgctgcaagt tttgtcagtt
    64801 cggcatcatc gatatcgata ccggtaatca ggcaaacgcc cttaccttta cgtccactgg
    64861 tctgacgctg aatacgcacc acaccgtcgc ctttagggcg tactgggggc gcttttggct
    64921 catcaatacg tccggtttct gttgagtaga ccagacggct gttggaatca ctcatcatgc
    64981 actccgctgt aaagaggcgt tgatcgcttt cagcgtctgc gctggatcta ccgattgcgt
    65041 taccgggcga ccaatcacca tataatcaac accagccgcc aacgcctgtt ctggcgtcat
    65101 aatgcggcgc tggtcaccag cttcactccc ctgcggacga atgcccggtg taaccagttt
    65161 gaactcctga ccgaatacct gtttaaagcg cacagcttcc tgagcagaac acaccacacc
    65221 atcaaggcca catttttgcg tcagtgccgc cagacgttct gcataatctg caggtgacag
    65281 tgtcacgcca agatcggcca ggtcgctggc ttccatgctg gtcaacactg tcacagcaat
    65341 caaaagcggt gcatctttgc catacggaac cagtgcctca cgcgctgcgg tcatcatacg
    65401 cgccccacca gaggcatgaa cattcaccat ccacacgcct aagtcagctg cagcagcgac
    65461 agcgtgcgct gcagtgttgg ggatatcgtg gaatttcagg tcaagaaaga tatcaaaacc
    65521 acgctgttga agttcgcgca caaactgtgg cccaaacaat gtaaacatct ctttgccgac
    65581 cttcagacga caatcgcgtg ggtcgatctt gtcgacaaag gccagcgcgg catcacgatt
    65641 atgataatca agggcaacaa ccacaggaga attcgtaaca gcgcgggaag aagatgaagc
    65701 agttaacgtc atgaccagac cttcttgatg atgggcaccg gaagtggcgc gaaacaggta
    65761 aacggcgagc attctacccg tggaaataaa aaattaacag ttgcgatttc tcccctggca
    65821 ggctttttct gcgccgtcgt tgccggagca caatggaaca tcaataacaa gtatgttgta
    65881 actaaagtcg gattttttta aaaattacag gccatcaaga ccgcgaatcg gtttaattgt
    65941 tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt
    66001 ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac gcagcaccat
    66061 caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg
    66121 gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcagcctc
    66181 actaccgtcg cgcgcttcga tgatatccgc aagcatcaat tcagcatcgg caccggtgtt
    66241 ctcttccacc gcgcgctgca ggaattctgc ccattcggca gttttaccca actgctgata
    66301 gcaggtttgc aacatttcca gcgtttcgct aaccagttct ctgtcctggg atatgacacg
    66361 ttgcagactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat
    66421 gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc
    66481 acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat
    66541 ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt
    66601 ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagcgcgc caatgcggaa
    66661 gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcatata acccggcggc
    66721 catgtaatca cgccccagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc
    66781 gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa
    66841 caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag
    66901 aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta
    66961 atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat
    67021 ataccagcca taggcagcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag
    67081 ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg
    67141 gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca
    67201 gaacaggcca caaatcaacc aaccgatagc aaaccccgca gcaaacaata ccgccagcaa
    67261 tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt
    67321 ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa
    67381 atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc
    67441 aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt
    67501 gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg
    67561 ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc
    67621 acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga tcgcgtggcc
    67681 aatgcatccc gagcagcagg cggcttccca tgactccagt tgcccagacc aacaagatag
    67741 caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagtagt gcccaactgg
    67801 cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac
    67861 gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt
    67921 ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccaaccaga
    67981 taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccaa
    68041 caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca
    68101 gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga
    68161 cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac
    68221 gccagccaga aatccatacg gctactggca taacaagcaa tagtgcagct cccactgcgg
    68281 tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga
    68341 aaatgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc
    68401 gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc
    68461 tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg
    68521 atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct
    68581 atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg
    68641 gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc
    68701 aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg
    68761 gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat actgtagagg
    68821 ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt
    68881 tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa
    68941 ttctgaccga agcggggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc
    69001 ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat
    69061 aaccctcttg cattgtgtaa ttaatttgct tgccggaagc aaaataaccg gcaagcaaat
    69121 acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag
    69181 gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacttcctg gctaccatcc
    69241 gcgcgcgtaa gcgtcaccgg aaccgtcgcg ccgggttgta gattttgcag atcgccaata
    69301 tcaatcttct cttccccggt tagccctaac gttttacgcg ttacgccttg cggaaattcc
    69361 agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca
    69421 atcaccacac gaattccaag cagacgcgga ccttttgccg cccagtcacg gcttgagcct
    69481 gatccatact ctttcccggc aattaccgcc agcggcgttt gctcctgctt atagcgcatc
    69541 gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca
    69601 acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact
    69661 tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct
    69721 tgtagatatc gacccgccgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca
    69781 gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccacaggt
    69841 gctggtgttg cctgcatttc atcaaagaaa ggcgataaac gaatataggt tgaatcctcc
    69901 tgccatccgt aggtatcgga tcgtgcgacg ttaatttctt tccactctgc agtgccttca
    69961 aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca
    70021 atttcttgtg ccgatggcca gatatctttc agataaaccg gatcgccttt gcgatcatgg
    70081 ccgataggct cagaagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc
    70141 agcggcggcg aggccagcca gttagtttta accagcggat ggatacggcc ttcaaagtta
    70201 cggttgccgg acagcaccgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata
    70261 ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta
    70321 aaccccagtt cgtcgagata cggtgtcagt ttcgcttttg ccagatagtc agaaacaact
    70381 ttcgagcctg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc cagagttacg
    70441 gcttttttcg ccagcaagcc tgcagccatc agcacacttg ggttagaggt gttggtgcac
    70501 gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata
    70561 acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg
    70621 gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt tggccctgcc
    70681 aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat cggttcatcg
    70741 cccgggttac gccacatgcc ctgcgctttg gcgtattttt cgaccaactc gacctgatct
    70801 tcactgcgcc cgcttaaacg catgtaatcg agggtgacag catcgattgg gaagaagcca
    70861 caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc caacggtagt
    70921 gaatccagac catcaccata aaattcgacg aatttcccga ccacgccatg tttgcgcagc
    70981 atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa ttttccggta
    71041 agtttgaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa cattgcggct
    71101 tctgcttcga tcccaccaac gccccacccc agcacgccaa ggccgttgat catggtggtg
    71161 tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcaccgtc ctgcaattca
    71221 ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc
    71281 ggcacgacgc taaaccgact gaacgcctgc tttccccatt tcaggaacac ataacgttcg
    71341 tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc accaaaacga
    71401 tcgacagtca ccgagtggtc aatgaccagg tcgaccggtg agagcggatt aacctttgca
    71461 gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac aacggcaggt
    71521 acgccggtaa agtcctgcat cagcaccctt gccgggcggt aggcaatttc acggtcagca
    71581 tgggcatttt tcagccatcc cgccagcgcg tggatatcct cttcggtaac cgagttacca
    71641 tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg acttgggtag acgggtgata
    71701 tcgcccagtg atttggcagc aagcggcagg ctgtagtagt ggtaagtttt atctttggcc
    71761 tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc ttaatgacag
    71821 ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa catcgcgtaa
    71881 cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa gcaaatgaca
    71941 accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt cggcccagaa
    72001 gagcatcgac agagaaaatg cacccatcca ggaccaatat ttcatatttg tgttgatcat
    72061 aggctacacc cttaacggtt attactttgt gttgaggtaa ctcaacctgt tattgctgcg
    72121 gtgtattaaa agccctttaa ttcattaaaa atgggtaata agaatgatga taattattgt
    72181 ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga tgttagttgc
    72241 cagtcgtctg gaataacgac ggaacattcc cgcttcgttt ctggttcatt gtcactatcc
    72301 tggcataatg gtgacgactc cagttgctcc tgaccaacca tagttatccc cacactttcc
    72361 aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc gccagccagg
    72421 ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc attgctaacc
    72481 tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat taatcatacg
    72541 atgagacaaa tcctaaagaa actttagtca aaaatccagc gaatttacga atcgtctcca
    72601 gtgaataatt caatcttttg acctgaaata gatatttgaa aactggaatt tgcgaggcgg
    72661 gtaattagac actac
//
LOCUS       DAZGCQ010000011        69751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000011 DAZGCQ010000000
VERSION     DAZGCQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.011"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..311
                     /gene="elaD"
                     /locus_tag="ACHK07_000789"
     CDS             <1..311
                     /gene="elaD"
                     /locus_tag="ACHK07_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO8101164.1"
                     /translation="HADLQQYLSQSCGAFVCMAAQEVIEQMESNSDSAPYTLLKNYAD
                     RFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDANINHYYRNLEIKHSQPKNRASSKRVS
                     "
     gene            complement(382..2109)
                     /gene="yfbK"
                     /locus_tag="ACHK07_000790"
     CDS             complement(382..2109)
                     /gene="yfbK"
                     /locus_tag="ACHK07_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO8101165.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     LMLKKQRESGVTLSTFGVGDDNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGVDPQGYRAEFIRLIEL
                     ADGVTDISQ"
     gene            2297..3217
                     /gene="yfbL"
                     /locus_tag="ACHK07_000791"
     CDS             2297..3217
                     /gene="yfbL"
                     /locus_tag="ACHK07_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO8101166.1"
                     /translation="MIIFYQPWVNALPSTPRHASPEQLEKTVRYLTQTVHPRSADNID
                     NLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASS
                     YENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPHFFRSDEMGS
                     AVHAASLEHPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIAVVGRMQDIN
                     AVRQVKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAIMITDTAFYRNKQY
                     HLPGDTADRLNYQKMAQVVNGVITLLYNSK"
     gene            3320..3823
                     /gene="yfbM"
                     /locus_tag="ACHK07_000792"
     CDS             3320..3823
                     /gene="yfbM"
                     /locus_tag="ACHK07_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO8101167.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(3890..5347)
                     /gene="nuoN"
                     /locus_tag="ACHK07_000793"
     CDS             complement(3890..5347)
                     /gene="nuoN"
                     /locus_tag="ACHK07_000793"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO8101168.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(5354..6883)
                     /gene="nuoM"
                     /locus_tag="ACHK07_000794"
     CDS             complement(5354..6883)
                     /gene="nuoM"
                     /locus_tag="ACHK07_000794"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO8101169.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(7047..8888)
                     /gene="nuoL"
                     /locus_tag="ACHK07_000795"
     CDS             complement(7047..8888)
                     /gene="nuoL"
                     /locus_tag="ACHK07_000795"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO8101170.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(8885..9187)
                     /gene="nuoK"
                     /locus_tag="ACHK07_000796"
     CDS             complement(8885..9187)
                     /gene="nuoK"
                     /locus_tag="ACHK07_000796"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO8101171.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(9184..9738)
                     /gene="nuoJ"
                     /locus_tag="ACHK07_000797"
     CDS             complement(9184..9738)
                     /gene="nuoJ"
                     /locus_tag="ACHK07_000797"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO8101172.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(9750..10292)
                     /gene="nuoI"
                     /locus_tag="ACHK07_000798"
     CDS             complement(9750..10292)
                     /gene="nuoI"
                     /locus_tag="ACHK07_000798"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO8101173.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(10307..11284)
                     /gene="nuoH"
                     /locus_tag="ACHK07_000799"
     CDS             complement(10307..11284)
                     /gene="nuoH"
                     /locus_tag="ACHK07_000799"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO8101174.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(11281..14007)
                     /gene="nuoG"
                     /locus_tag="ACHK07_000800"
     CDS             complement(11281..14007)
                     /gene="nuoG"
                     /locus_tag="ACHK07_000800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO8101175.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(14060..15397)
                     /gene="nuoF"
                     /locus_tag="ACHK07_000801"
     CDS             complement(14060..15397)
                     /gene="nuoF"
                     /locus_tag="ACHK07_000801"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO8101176.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(15394..15894)
                     /gene="nuoE"
                     /locus_tag="ACHK07_000802"
     CDS             complement(15394..15894)
                     /gene="nuoE"
                     /locus_tag="ACHK07_000802"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO8101177.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(15897..17699)
                     /gene="nuoC"
                     /locus_tag="ACHK07_000803"
     CDS             complement(15897..17699)
                     /gene="nuoC"
                     /locus_tag="ACHK07_000803"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO8101178.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(17793..18455)
                     /gene="nuoB"
                     /locus_tag="ACHK07_000804"
     CDS             complement(17793..18455)
                     /gene="nuoB"
                     /locus_tag="ACHK07_000804"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO8101179.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(18471..18914)
                     /gene="nuoA"
                     /locus_tag="ACHK07_000805"
     CDS             complement(18471..18914)
                     /gene="nuoA"
                     /locus_tag="ACHK07_000805"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO8101180.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(19545..20483)
                     /gene="lrhA"
                     /locus_tag="ACHK07_000806"
     CDS             complement(19545..20483)
                     /gene="lrhA"
                     /locus_tag="ACHK07_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO8101181.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            21403..22620
                     /gene="alaA"
                     /locus_tag="ACHK07_000807"
     CDS             21403..22620
                     /gene="alaA"
                     /locus_tag="ACHK07_000807"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO8101182.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            22704..23303
                     /gene="yfbR"
                     /locus_tag="ACHK07_000808"
     CDS             22704..23303
                     /gene="yfbR"
                     /locus_tag="ACHK07_000808"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8101183.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(23362..25194)
                     /gene="yfbS"
                     /locus_tag="ACHK07_000809"
     CDS             complement(23362..25194)
                     /gene="yfbS"
                     /locus_tag="ACHK07_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO8101184.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(25281..25931)
                     /gene="hxpA"
                     /locus_tag="ACHK07_000810"
     CDS             complement(25281..25931)
                     /gene="hxpA"
                     /locus_tag="ACHK07_000810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO8101185.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLDQITVTKQPNGDVIIQ"
     gene            complement(25942..26436)
                     /gene="yfbU"
                     /locus_tag="ACHK07_000811"
     CDS             complement(25942..26436)
                     /gene="yfbU"
                     /locus_tag="ACHK07_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO8101186.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(26519..26974)
                     /gene="yfbV"
                     /locus_tag="ACHK07_000812"
     CDS             complement(26519..26974)
                     /gene="yfbV"
                     /locus_tag="ACHK07_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO8101187.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            27312..28514
                     /gene="ackA"
                     /locus_tag="ACHK07_000813"
     CDS             27312..28514
                     /gene="ackA"
                     /locus_tag="ACHK07_000813"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO8101188.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKDSGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            28589..30733
                     /gene="pta"
                     /locus_tag="ACHK07_000814"
     CDS             28589..30733
                     /gene="pta"
                     /locus_tag="ACHK07_000814"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8101189.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            30923..32443
                     /gene="yfcC"
                     /locus_tag="ACHK07_000815"
     CDS             30923..32443
                     /gene="yfcC"
                     /locus_tag="ACHK07_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO8101190.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVIFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(32476..33018)
                     /gene="yfcD"
                     /locus_tag="ACHK07_000816"
     CDS             complement(32476..33018)
                     /gene="yfcD"
                     /locus_tag="ACHK07_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO8101191.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(33076..33627)
                     /gene="yfcE"
                     /locus_tag="ACHK07_000817"
     CDS             complement(33076..33627)
                     /gene="yfcE"
                     /locus_tag="ACHK07_000817"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO8101192.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(33683..34327)
                     /gene="yfcF"
                     /locus_tag="ACHK07_000818"
     CDS             complement(33683..34327)
                     /gene="yfcF"
                     /locus_tag="ACHK07_000818"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO8101193.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            34463..35110
                     /gene="yfcG"
                     /locus_tag="ACHK07_000819"
     CDS             34463..35110
                     /gene="yfcG"
                     /locus_tag="ACHK07_000819"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO8101194.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            35167..35529
                     /gene="folX"
                     /locus_tag="ACHK07_000820"
     CDS             35167..35529
                     /gene="folX"
                     /locus_tag="ACHK07_000820"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO8101195.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            35550..36443
                     /gene="yfcH"
                     /locus_tag="ACHK07_000821"
     CDS             35550..36443
                     /gene="yfcH"
                     /locus_tag="ACHK07_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO8101196.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCTRWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(36491..37381)
                     /gene="rpnB"
                     /locus_tag="ACHK07_000822"
     CDS             complement(36491..37381)
                     /gene="rpnB"
                     /locus_tag="ACHK07_000822"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO8101197.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLTAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(37578..38351)
                     /gene="hisP"
                     /locus_tag="ACHK07_000823"
     CDS             complement(37578..38351)
                     /gene="hisP"
                     /locus_tag="ACHK07_000823"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO8101198.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGAIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(38359..39075)
                     /gene="hisM"
                     /locus_tag="ACHK07_000824"
     CDS             complement(38359..39075)
                     /gene="hisM"
                     /locus_tag="ACHK07_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8101199.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(39072..39758)
                     /gene="hisQ"
                     /locus_tag="ACHK07_000825"
     CDS             complement(39072..39758)
                     /gene="hisQ"
                     /locus_tag="ACHK07_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO8101200.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(39848..40630)
                     /gene="hisJ"
                     /locus_tag="ACHK07_000826"
     CDS             complement(39848..40630)
                     /gene="hisJ"
                     /locus_tag="ACHK07_000826"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO8101201.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQKIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(40851..41633)
                     /gene="argT"
                     /locus_tag="ACHK07_000827"
     CDS             complement(40851..41633)
                     /gene="argT"
                     /locus_tag="ACHK07_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO8101202.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(41899..42468)
                     /gene="ubiX"
                     /locus_tag="ACHK07_000828"
     CDS             complement(41899..42468)
                     /gene="ubiX"
                     /locus_tag="ACHK07_000828"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO8101203.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(42563..44080)
                     /gene="purF"
                     /locus_tag="ACHK07_000829"
     CDS             complement(42563..44080)
                     /gene="purF"
                     /locus_tag="ACHK07_000829"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO8101204.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(44117..44605)
                     /gene="cvpA"
                     /locus_tag="ACHK07_000830"
     CDS             complement(44117..44605)
                     /gene="cvpA"
                     /locus_tag="ACHK07_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO8101205.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(45046..45708)
                     /gene="dedD"
                     /locus_tag="ACHK07_000831"
     CDS             complement(45046..45708)
                     /gene="dedD"
                     /locus_tag="ACHK07_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO8101206.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(45698..46966)
                     /gene="folC"
                     /locus_tag="ACHK07_000832"
     CDS             complement(45698..46966)
                     /gene="folC"
                     /locus_tag="ACHK07_000832"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO8101207.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(47036..47950)
                     /gene="accD"
                     /locus_tag="ACHK07_000833"
     CDS             complement(47036..47950)
                     /gene="accD"
                     /locus_tag="ACHK07_000833"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO8101208.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(48106..48765)
                     /gene="dedA"
                     /locus_tag="ACHK07_000834"
     CDS             complement(48106..48765)
                     /gene="dedA"
                     /locus_tag="ACHK07_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8101209.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(48848..49660)
                     /gene="truA"
                     /locus_tag="ACHK07_000835"
     CDS             complement(48848..49660)
                     /gene="truA"
                     /locus_tag="ACHK07_000835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO8101210.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRFDLPKPPMGPLFLAD"
     gene            complement(49660..50673)
                     /gene="usg"
                     /locus_tag="ACHK07_000836"
     CDS             complement(49660..50673)
                     /gene="usg"
                     /locus_tag="ACHK07_000836"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8101211.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(50739..51875)
                     /gene="pdxB"
                     /locus_tag="ACHK07_000837"
     CDS             complement(50739..51875)
                     /gene="pdxB"
                     /locus_tag="ACHK07_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO8101212.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKKAGIGFSAAPGCNAIAVVEYVLSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRNLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHIALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            51974..52969
                     /gene="flk"
                     /locus_tag="ACHK07_000838"
     CDS             51974..52969
                     /gene="flk"
                     /locus_tag="ACHK07_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO8101213.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(52966..54144)
                     /gene="yfcJ"
                     /locus_tag="ACHK07_000839"
     CDS             complement(52966..54144)
                     /gene="yfcJ"
                     /locus_tag="ACHK07_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFO8101214.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(54453..55673)
                     /gene="fabB"
                     /locus_tag="ACHK07_000840"
     CDS             complement(54453..55673)
                     /gene="fabB"
                     /locus_tag="ACHK07_000840"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO8101215.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            55832..57838
                     /gene="mnmC"
                     /locus_tag="ACHK07_000841"
     CDS             55832..57838
                     /gene="mnmC"
                     /locus_tag="ACHK07_000841"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO8101216.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHLHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEVPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLVEQKDKAVSAPVYDDLFMLDALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(57959..58237)
                     /gene="yfcL"
                     /locus_tag="ACHK07_000842"
     CDS             complement(57959..58237)
                     /gene="yfcL"
                     /locus_tag="ACHK07_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO8101217.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(58271..58819)
                     /gene="epmC"
                     /locus_tag="ACHK07_000843"
     CDS             complement(58271..58819)
                     /gene="epmC"
                     /locus_tag="ACHK07_000843"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO8101218.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(58819..59628)
                     /gene="yfcA"
                     /locus_tag="ACHK07_000844"
     CDS             complement(58819..59628)
                     /gene="yfcA"
                     /locus_tag="ACHK07_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO8101219.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(59628..60452)
                     /gene="mepA"
                     /locus_tag="ACHK07_000845"
     CDS             complement(59628..60452)
                     /gene="mepA"
                     /locus_tag="ACHK07_000845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO8101220.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKRVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(60456..61541)
                     /gene="aroC"
                     /locus_tag="ACHK07_000846"
     CDS             complement(60456..61541)
                     /gene="aroC"
                     /locus_tag="ACHK07_000846"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO8101221.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(61576..62508)
                     /gene="prmB"
                     /locus_tag="ACHK07_000847"
     CDS             complement(61576..62508)
                     /gene="prmB"
                     /locus_tag="ACHK07_000847"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8101222.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            62674..63225
                     /gene="smrB"
                     /locus_tag="ACHK07_000848"
     CDS             62674..63225
                     /gene="smrB"
                     /locus_tag="ACHK07_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO8101223.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(63339..64178)
                     /locus_tag="ACHK07_000849"
     CDS             complement(63339..64178)
                     /locus_tag="ACHK07_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO8101224.1"
                     /translation="MKIKVILFGMLLFGLNSSVQADVTFNNVQTYGASYGSGLTTSIV
                     YKIDVLTPEGAYYGTYSQGGIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCP
                     GMTSGWVCHRLPVNVHVTGTTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPT
                     EPVASYDISWDENTVVHNKSLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYC
                     RLVSQLMTFSVQGCDNNKVNVTANRHPVTDKQLHDMQVRVDTSERQSFDATCRFQYIL
                     NEL"
     gene            complement(64179..64697)
                     /locus_tag="ACHK07_000850"
     CDS             complement(64179..64697)
                     /locus_tag="ACHK07_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101225.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGVSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
     gene            complement(64700..65176)
                     /locus_tag="ACHK07_000851"
     CDS             complement(64700..65176)
                     /locus_tag="ACHK07_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101226.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            complement(65173..65676)
                     /locus_tag="ACHK07_000852"
     CDS             complement(65173..65676)
                     /locus_tag="ACHK07_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101227.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            complement(65692..66444)
                     /locus_tag="ACHK07_000853"
     CDS             complement(65692..66444)
                     /locus_tag="ACHK07_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8101228.1"
                     /translation="MTNLFCSAKKSTMLLALLLTTASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTRSGQEFTVNNPTPYYVVISNASTQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            complement(66467..69106)
                     /locus_tag="ACHK07_000854"
     CDS             complement(66467..69106)
                     /locus_tag="ACHK07_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8101229.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDVTHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNIMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQSDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPMIQEQTQQVMPTEAPVSVSAN"
     gene            complement(69188..69751)
                     /gene="yfcV"
                     /locus_tag="ACHK07_000855"
     CDS             complement(69188..69751)
                     /gene="yfcV"
                     /locus_tag="ACHK07_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101230.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        17273 a        18852 c        17373 g        16253 t
ORIGIN      
        1 tacatgccga tcttcagcaa tacttaagcc aaagttgcgg tgcatttgtg tgcatggcag
       61 cccaggaagt gattgaacaa atggaaagca attctgacag cgccccctat acgttattaa
      121 aaaactatgc tgacagattt aaaaaatatt cagcagaaga gcagtacgaa attgattttc
      181 aacatcgact ggtaaacaga aattgttatt tagataaata tggcgatgca aatatcaatc
      241 attattatag aaacttagaa ataaaacact cacaacccaa aaatagagca tcaagcaaaa
      301 gagtaagtta acatcattgt taattaattc gaccagtcag caaatatgct gactggtcga
      361 acatagtttg ctaaaaagtc atcactgact gatgtcagtc acaccatccg ccagttcaat
      421 caggcgaata aattccgccc tgtaaccctg tggatctacc ccttttgcct gctgagccca
      481 ctgtttgatc tgctgccagg aggtattgtt caggtattca gaaccgcgta acttttgccc
      541 atatgcagct actgctgcgc gaaaacgcat atcttcagag ggcgcgttta ttgttggccc
      601 cagcgggaat tcaactaact gactttcttt tccctgcggg tatttccagc gaatttttaa
      661 ccaggccagt tctttcgttt tgtccgattt cgctaatttg ttatccgggg catagcgtaa
      721 cttatcaatt gatgcttttt gcccgttcag cgttaattca aataacaacg ttatatgttt
      781 gcctgcgcca atatcccccg catcaacgtt gtcgttatta aaatcttccg cccgaagttg
      841 gcgcttttca taaccaatct gacggtattc cgttacccac gcggggttaa actcaatttg
      901 cgctttgaca tcttttgcta cggtaatcaa cgtctgccgc atttcactgt tcaatacttt
      961 ctgcgcttca gagagggtat caatgtagct gtagttaccg ttgccaacat cggcaattcg
     1021 caccatcatt gcctcgttgt aattgtcatc ccccacgcca aacgtcgaca gagtaacacc
     1081 ggactcccgc tgttttttga gcattaattc aatcgatttt ggatcgtcaa taccaacgtt
     1141 aaagtcaccg tcagtggcca ataaaatgcg attgataccg cccttaataa accccttcgc
     1201 cgcctgctga taagccagtt ccagcccggc accgccattg gtactgcctt cggcatccag
     1261 cgaatcaatt gcggcattaa tttccgcctt atgactcccg gagatagaag gcaatgcaat
     1321 acgggagtcg ccagcgtagg tcacgatggc aatgttatcc tgctcacgaa gttctttgac
     1381 caataatttc aacgaagact ggataagtgg caaacgttca tcagaaatca ttgaaccaga
     1441 agtgtcgata agaaagacca gattagaagc tggtaactct tcacttttgc gatctttcgc
     1501 caggatatca actttcagca atgttcgctg ttcattccat ggtgcaggtg ccaattcgta
     1561 gcgcatagcg aaaggtattg gcttagaggc cggaatagat tgtttgtctt tgatatccca
     1621 atcagacggg aaataattga ctatctcctc cacccgcaca gcgtctggcg gaggtaacag
     1681 cccttgattg aggaaacgcc ttacattcgc ataactgcca gtgtcaacgt caagactaaa
     1741 cgtcgccaac ggattttgcg ctacctgctt aaccggatta tcatcgaact gctggtagcg
     1801 agcggttcct ggatttgcga tatgtgtagc ttttgcttta gccgctcttg caaatgttgg
     1861 cgcttcctgc aatcgcccct gtaaagcctg tttgtctgaa tattgttgca cttcttgctg
     1921 ggccaaggct ttcgcggcgg cggcgctttg ctcagcctct tttattgcag cttgctgcgc
     1981 ggcaagcact tgctgctctg tgggagtact gggttgttgt tgctgacttt ccttattctc
     2041 aggttgcggc ccacatcctg acaaaatcaa actactcata agcaacatga ttatattttt
     2101 atttcgcata taaaatcctt ttaaataaac tcaggcacca gacataaaat atactctttg
     2161 tataatcctg ctaatcttaa taaaatacaa tatcgaaacc catagcaaat aatgattcaa
     2221 caacgcaacc cattaataat ttgcatataa aaaaataatc tttgctttta ttatattatt
     2281 tgtgttttta ctccctatga ttatttttta ccaaccctgg gtcaatgcat taccgtcaac
     2341 gccccgacac gcaagtcctg agcaattaga aaaaacagtt cgttatctta cacaaactgt
     2401 gcatccacgt agtgccgaca atattgataa tctgaatagg tccgcagagt atataaaaga
     2461 ggtctttgtc agtagcggtg ccagagttac ctcgcaggat gtccccatta cgggtggccc
     2521 ctacaaaaac attgttgctg attatggtcc tgccgatgga ccgctgatta ttattggtgc
     2581 gcattatgac tctgccagca gttatgaaaa cgatcaattg acctatacgc cgggcgcgga
     2641 tgataacgcc agcggtgtgg caggattact cgaactggca cgtttgttac atcagcaagt
     2701 accgaaaaca ggcgtgcagc tggtcgccta tgcgtcggaa gaaccgcact tctttcgtag
     2761 cgatgaaatg gggagcgcgg tgcatgcagc ttcgcttgag catccagtga aattaatgat
     2821 agcactggag atgattggct attacgactc tgcgcctgga agccagaatt acccttaccc
     2881 ggcaatgtcc tggctttatc ccgatcgggg agactttatt gccgtggtcg gcagaatgca
     2941 ggatatcaac gccgttcgtc aggtaaaagc ggcattgttg tcatctcagg atttatctgt
     3001 ttattctatg aatgccccag ggtttattcc cggtattgat ttctctgatc acctgaatta
     3061 ttggcaacac gatattcccg ccataatgat tactgacacc gctttttatc gtaataaaca
     3121 ataccacttg cccggtgata ccgcagacag attgaattat cagaaaatgg ctcaggtagt
     3181 gaatggtgtt ataactttgt tatacaacag taaataataa aaatttctcg aggtgggtcc
     3241 agaaaaagcg acatgcacat tttattttcc ctcatctgtt ttaagatgcg aaacatgaaa
     3301 ttcaacggag gaaaaataaa tgggaatgat tggctatttt gcagaaattg actcagaaaa
     3361 gattaaccaa ctgcttgagt ctactgaaaa acctttgatg gataatatac atgacacgct
     3421 ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact ttgggctgac
     3481 aggcacctct gcgttcgatc cagcgaagaa tgatccgctg agtcgtgccg tgcttggtga
     3541 acacagcctc gaagatggta tagatggttt cctgggattg acatggaatc aggagttagc
     3601 cgcgacaatc gaccgactgg aatcactgga tcgaaatgaa ctgcgtaaac agttttccat
     3661 taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg
     3721 gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat
     3781 gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa
     3841 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actaacagat tacatcagtg
     3901 gcattgctaa ccgcacaatg ctaatcagcg gttgtggcca tacacccagc accagtacca
     3961 acagtgcgga gatcagcacc acgataccgc ccgcgctgta ctgccagttt gacggtgcat
     4021 cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt
     4081 agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacaag tgtgcctgga
     4141 caccgaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca
     4201 gagacagcat catcaccgtc atcaccgccg cgaggatcgg acgatgccag aacagaccgc
     4261 ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca
     4321 cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct accgcttcca
     4381 tcgacatctc gccggtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     4441 tagatgagta accgagcaga cgtttgatgt tggtctggct cagcgccatc aggttaccga
     4501 agatgatgga ggcaaaggcg ataatcgcca gcaccacccg aatcgcttcg ctgtcaccca
     4561 ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg
     4621 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     4681 acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa cctgccagca
     4741 acagcggctc gttgagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt
     4801 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     4861 aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     4921 ccaggccaaa cagcggcaaa gagatcagtt caataccgag gaacagagac gccagatggt
     4981 ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat
     5041 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     5101 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa
     5161 cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     5221 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     5281 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     5341 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     5401 tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     5461 gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct
     5521 ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgtaacatc gccagcgaat
     5581 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     5641 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg
     5701 ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc cacagaccgc
     5761 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccgcag agaataaaca
     5821 gacccgccgc tgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     5881 ggctgccggt gtagatagca atcagtacga agcccatgtg ggaaaccgag gtgtaggcga
     5941 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa
     6001 cacccagcca catagcgatt ggcgcgaact ctgccgacgc atttgggaac agcggcaggg
     6061 agaaacgcag caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg tcaacggaac
     6121 cggcggtcgg agcctgggag tgcgcatccg gcagccagcc atgcagcgga accaccggca
     6181 ttttgactgc gaaggcgatg aagaagccca gcatcaacag atattccaca ccactggaca
     6241 ttggcgtatt caacagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa
     6301 caaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga
     6361 agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg
     6421 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaaaaagaac aggaacatgt
     6481 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     6541 agccctgata tttttcgatc tctttccacg aacagagtac cgccagcaca ccgagcagac
     6601 cggtcagcac gaccattagc agcgacagcc cgtcaatggc gagatggata gagataccaa
     6661 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     6721 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     6781 tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     6841 agccgccgat aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt
     6901 gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg
     6961 actgggggta gtgtcggatg caccgcgaag gaggcttatc cgacctacgt tcttatatgc
     7021 ccaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg
     7081 accgcaccga tgctcatgga tgccacatac cagcgcagat aaccgttctc gcttaacagc
     7141 aggcctttac ctgcaaagcg ggaaagaacc gccggaatgt tcatcattga gttcagcgga
     7201 tcgcgtttca gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcgtacagc
     7261 cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca gacggcccgg cgcactgttg
     7321 gcgatggagg tcaccagagt acgtttaccc agccacagcc aggctgccag cagaatgccg
     7381 accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc
     7441 gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa
     7501 aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc gtgagcgtga
     7561 atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag cgaggtcata
     7621 aacgcaccga ccagacctgc caccatcaga ttgatatgac cattcgccat cgcacccgcg
     7681 aggatctcat ccttactgaa gaagcccgca gtgaccagcg gcagtgccga cagtgctgcg
     7741 ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc gcccatcttg
     7801 aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc
     7861 gctttaaaga acgcgtgggt catcaagtga aaaatcgccg catcccatgc ctgcacgcca
     7921 agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg
     7981 tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc gacaataccc
     8041 accagatgca gaacttccgg cgtcatcagg aacaggccgt gggtacgggc gatcaggtag
     8101 acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc
     8161 atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag atttaccgac cgcaccgccc
     8221 agcagcatca gcgtcgccca catcagcata ttattgccgt cagcaaagtg cgctggtgcc
     8281 agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagcgcg
     8341 aaagcgagga acacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcgcca
     8401 ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc
     8461 cagccgaggt acatcagcag caggttgtcg gcaagcacca gaactaccat gctggcgatg
     8521 aacaggttgg tgtaggcgaa gaagcgagag tagccctctt caccgcgcat gtaccaggag
     8581 gcgtacatgt ggatcaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg
     8641 ccgtccagca ccagattgaa accgatgtta aagtcgccta ccgacatcca cgtccacagc
     8701 ggctggctgt aagcctgctc gccgttagcg aagaaatcaa cgccgataaa agcggttacc
     8761 agcgccgcca ggcccacaga gccaacgccg acgatcgccg aaacgttttc agaccagcgc
     8821 ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc
     8881 atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga cgacggtgaa
     8941 gttgcagcag cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg atggcgagaa
     9001 tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca aaggccagcg
     9061 cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa
     9121 ccagaccggt taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg
     9181 ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac
     9241 ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag
     9301 catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt
     9361 agcactgatt ggcgtaccgt cgataccttg atcgttaaca ccgagaatgg cgtaaacaat
     9421 caccaccaac atgatggctg acaaaattgc cggaccaatc cacacctgcg gtttcagcca
     9481 ctggcgttct tgttcgattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa
     9541 cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcga agtaagcgcc
     9601 tagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg
     9661 taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat
     9721 ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc
     9781 ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg
     9841 tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc tttctcgtaa
     9901 accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg
     9961 gtcggacagg cttcttcgca caaaccacag aaaatgcagc gtgagaagtt gatgcggaaa
    10021 aattccggat accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc
    10081 gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg
    10141 gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt
    10201 cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg
    10261 ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc
    10321 agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc
    10381 agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga
    10441 acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc
    10501 cttgccagcc accgaagaac agcgtcacca tcaatgcaga gatggtcaca atcccgatgt
    10561 actcacccac gaagaacaga ccgaacttca tgccggaata ttcaatgtgg taaccatccg
    10621 ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacacacc gccacgcccg
    10681 cgatggcaaa ggtaataaaa ccaaagaatt gcgggatgac gttccacaca tgcgcctggc
    10741 tgttgacgat gtcggtcatg ttgaatgaac cggcttgcgc caccacgccc atcagggaaa
    10801 gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca cccagcagcg
    10861 agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc aaaccggcca
    10921 tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc ggactgactg
    10981 gcacaatcgc aaaggccagc agcagtgagg taaaggcaat catcggtgcc agggtaaaga
    11041 tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacattttg atcatatccg
    11101 cgaccagctg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg ttctggaaca
    11161 gacccagcag gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca accaccagca
    11221 ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt gatatccaac
    11281 tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg gagcaatgcc
    11341 ggacataccc atcggcaagc ccacctgccc tgccgtcagc ccttcagcga tttcaaccgg
    11401 cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt gtacctgcgt tcacacccaa
    11461 cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacggc tctggaagac
    11521 cggagcacgc tgtgacaatt catcactgcc aaacaggtgg taatacggcg caatacgcca
    11581 tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccattctc
    11641 gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc
    11701 ctggaatttg ttccacgcct gcggggagtt ccagcctggc gcccaggcaa acggcacttg
    11761 cgaacggtgc gcggtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat
    11821 atcctgcggc tgacgcggct catgaacgct aatattggcg cgcatggcgg tacgaccgct
    11881 gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc
    11941 agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa tcacatggtc
    12001 gagctgcgtc cagtccactt cacggctcaa cagggtactg tgcagcgagt gtaaccagcg
    12061 ccagctttcc agcatgacag ttttgctgtc gtaataagcc ggatcgtaaa cctggaagaa
    12121 acgttgggcg cgaccttcat tattaattac cgtaccgtcg ctttcagcaa agctggcagc
    12181 ggaaagtacc agatgggcgt tttccatgat cgctgtgcgt tgatggtcaa ccaccatcac
    12241 cagcggcgct ttagccagcg cagcattcac gcgggtagca gaagcgtgac gatgcagatc
    12301 gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcggtta acgcttcttc
    12361 aagcgatcca ccgcccataa tgcccagccc catgctgttg acggaacggg caatcatggt
    12421 gataccgacg tcagcgccgc gacctttcag ggctttcgcg acgttagccg cagcctgaat
    12481 tacttcagcg ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg cacccgccag
    12541 tgcctgcacg atgacgtcga ttttgctttg cagttcaggt tcaataccgt caaccgctgg
    12601 cgcggagtta tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg
    12661 tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa
    12721 cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc agtcagccac
    12781 tttctgtgct gccgccattt cgcgcgcttt acctttcacc gcctgacgca ctgccagcgc
    12841 gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat cgtaagattc
    12901 gatttcgcgc agagcaggag tataaatgcc gccttcacgc agtactttca gcgccagttg
    12961 cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc ccaccagctc
    13021 acgcagcgca aagttacttt ccacgctggc gcgcggagaa ccaatgccga tcactttctt
    13081 cgactgacgc agaatatctg ctgcgccctg cattgcctgt tcggcgttga gggtaatgaa
    13141 atcatcgcca cgacgctgta ctggctgacg cggacgatct ttcaggttga cgtaaccgta
    13201 accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt aacggttttc
    13261 gatacgacgc agttcgccat aacgctcgcc ggggctgatg ttacagccga tggaacattg
    13321 ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt
    13381 tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc
    13441 cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcgccat aaacgcccag
    13501 atctgtaccg tcagcgtaat ctttgtagta acgcacgcag cggtagcagg cgatgcagcg
    13561 gttcatttcg tgagagatga acggccccaa atcctgatta cggtgggtac gtttggtgaa
    13621 acggtagcga cggaagctgt gtccggtcat cacggtcata tcctgaagat ggcagttacc
    13681 gccttcttca cataccggac agtcgtgcgg gtggttggtc attaaccact cgaccacact
    13741 ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg
    13801 tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac
    13861 cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat
    13921 atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc
    13981 tttgccgtct acatgaattg tagccattag catgcttcca gttttctcag tcagagactg
    14041 agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa
    14101 tcaaatgggt attgctgaac ggctgtttga tccccgcctc aaattcttcg cggaaatatt
    14161 tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg
    14221 ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggctga ccttcgccac
    14281 gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc
    14341 agccgcagga ctcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga
    14401 tctcatggtc aaccgccatc gccagcgccg tacccagacg gcttcccgct ttaccgatac
    14461 tttcgaattc catcggcaga tcaaggtgcg cttcggtcag gaagtcagtc cccgcgccgc
    14521 ctggctgcca ggctttaaat ttcagaccat cacgcatacc accggcgtaa tcttcgagga
    14581 tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc cggatttttc acccgaccgg
    14641 agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca
    14701 cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgttg acacaggtcg
    14761 gtttgcccca tacgccggag gttgccggga aggggggttt cgagcgtggg ttagcacgac
    14821 gcccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc cctgccccgg
    14881 tatggacgaa cagttcgaag tcgaaacctg ttcccataat gtttttgcca agcaagcccg
    14941 cttcagtggc ttcggcaatg gcgcggcgca gatttactgc cgcttcgata tattcgccgc
    15001 gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagatgagc ataccttcca
    15061 ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt ataggtgccc ggctccattt
    15121 catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc atcaggctcc
    15181 acttcaggcc agtcgaaaag cccgcgccgc cgcgcccttt cagaccagcg tcttttacct
    15241 gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcccct tcgtaaccgt
    15301 ttttgctgcg gtattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca
    15361 gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg
    15421 gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg atcatcatgt ttggcccttt
    15481 atcacagttc cccaggcagc aagttggcag cagcgtaaag cggccatcaa atgtcgtttg
    15541 ccctggtttg atgttcagct ttttctcgag cgccgcctga ataccctgat acccgttgat
    15601 atgacagacc acgctgtcgc aataacggat cacatggcga ccaaccggct gacggaagat
    15661 ctgactgtag aacgttgcca caccttcgac gtcgcttgcc ggaataccca gcacatcggc
    15721 gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc ttctgaacga ttttcagcgc
    15781 ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg
    15841 ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag
    15901 cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc
    15961 aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtgcggata
    16021 cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt gatccccttg
    16081 gtcgcctcaa tcatctggaa agattcattg gcaggcatca ccggccccca cgacacttgc
    16141 aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggtggcgtg
    16201 gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga
    16261 atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta gcagtcagaa
    16321 acaccaccac caaccgggat ttcaaagtcg aagttttcat agccagaata aggacgcgcc
    16381 ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg cgccagtggt gccccactcc
    16441 agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag aatggtattt
    16501 tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc
    16561 agcaggcgat cccagccacg cggcaggtca tgcgctacac cgccaatacg gaaccacgcc
    16621 gggtgcatac ggaaacccgt gattgcttcc accagatcgt aaattttctg acgatcggta
    16681 aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa taaaggtcga gatatacagc
    16741 aggtgactgt tgatgcggaa cagttcggag agcattacgc gaatgacgtt aacgcgatcc
    16801 ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc gttaacgcag
    16861 ccgccgaggt attcgatacg gtcggtatac ggaatgtagc tgtgccagga ctggcgttcg
    16921 cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc gacaatctct
    16981 tcgccgtcga gctgcaaaac gatacggaaa gcaccgtgcg ccgacgggtg gttcggaccg
    17041 aggttgagga acatgaagtc ctcgttttcg gtgccgcgct tcatccccca ctcttccggt
    17101 ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc aaacggcgag
    17161 aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca ggtttgcggc
    17221 atcatgatgc ggcgcaggtt cgggtgaccg tcgaatgtaa tgccaaacag atcccaggtt
    17281 tcacgctcat accagttagc gttcgggaac agtttggtga aggtcggtac gtgcaggtcg
    17341 ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg
    17401 tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc
    17461 atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgcca
    17521 acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga
    17581 acagtaaagg catccggccc aaaacggttg cgcagttcgc caattaccgg atcatcaaga
    17641 tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt
    17701 gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag
    17761 cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg
    17821 gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa
    17881 acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat agattcctgc
    17941 aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca
    18001 tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccacca
    18061 gagttggcac aggcacccat tgagataacc cattttggtt ccagcatctg gtcatacaga
    18121 cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat caggtcagcc
    18181 tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta
    18241 aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag
    18301 tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact
    18361 tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca
    18421 ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga
    18481 cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc
    18541 cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa
    18601 attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga ccatgcgaac
    18661 agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg
    18721 gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg cacgtttttc
    18781 gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg
    18841 ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat
    18901 gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct cttcacggca
    18961 gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc actgtagaaa
    19021 ttcttttata cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt
    19081 taacaaaagc ttcttcacat tagtttacat aatatcaaca ccattagcat ttaatgccct
    19141 ttcaccccag atccttgacg actccaggat aattagatgt tgttgaatcg tgtccgttgt
    19201 gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca
    19261 aaagagagta ttttcttgat ctgtgacacg cttttgtcat tccataacaa aaacgcagca
    19321 acaaatttac gtatttttta acatcattgt agcaggtgat ttttttcagg cgattatttg
    19381 tgcgtacggg gcgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcacg
    19441 atgtgaagaa ggttctttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct
    19501 cgttttttac actattgtct caggaattat ctatggtccg tcgattactc gatatccctt
    19561 tcaatcaaca atgaatcatc cccttccgga gcagatattg ggctgtattg ccacggatta
    19621 tggtagctct ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca
    19681 tagcacagca aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca
    19741 cgcaagtccg gactcatcat ctcgaccggc cttgccgtca cgccaagacc ggctttcact
    19801 gccgcacgaa cggccggaag cgtcgaggcg acataagcca gtcgccaggg aatatctgct
    19861 ttattaagcg tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga
    19921 gggatcggct cacctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag
    19981 gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact
    20041 tcctgggatt ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct
    20101 aatttcggat aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat
    20161 tcatctgaag caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag
    20221 gcctcatcat taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca
    20281 gttaacagtt tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga
    20341 cgctgcattt gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca
    20401 aaagtgttca gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt
    20461 atcggacgat ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt
    20521 aatgctgtgc acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat
    20581 ccggaacccg gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg
    20641 ttccgttata atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta
    20701 agttactcac tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc
    20761 cttgttccac tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt
    20821 gaggcggcat caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt
    20881 tatttataag ggtcattgcg aattatctga tgcaaagtta tgttatgtta ggcaaagtaa
    20941 tcttctatta ttaataagca catcaaaacc tttttgaata ttaaataata attaattagc
    21001 atcatcctca ttcattaatt ccgcttaaca atagtttcac accttgcccc cattgcgaca
    21061 cctcggaaat catcacgcgg ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc
    21121 attagtgaat tcttaaatga ggcattttca cacaattatc ttacagataa aaaaaccaga
    21181 cttacaatta agaatcagaa caattcacta tataacattg catgtaaagc atatacacct
    21241 cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat
    21301 cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc
    21361 cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa
    21421 tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa
    21481 cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc
    21541 ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa
    21601 gggtattgcg attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactatcag
    21661 gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag
    21721 cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca
    21781 ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat
    21841 ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg
    21901 cctcgtacgc gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc
    21961 aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc
    22021 gatgaaattt atgacaaaat tctctacgac gacgctgagc atcactcaat tgcgccgctg
    22081 gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc
    22141 ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa
    22201 ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt
    22261 cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt
    22321 tatgagcagc gtaaccgcgc gtgggaattg atcaacgata ttccgggcgt ttcctgcgtg
    22381 aaacctcgtg gtgcgctgta catgttcccg aaaatcgacg ccaaacgctt taacattcac
    22441 gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg
    22501 acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat
    22561 gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa
    22621 tcttaatttc actgccggag attgcatccg gcagtgttat cccgccacaa tgacctgatg
    22681 atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc
    22741 tgaaactcat taaccgctgg ccgctcatgc gcaacgtgcg gacggaaaat gtgtccgaac
    22801 acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg
    22861 gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag
    22921 tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt
    22981 acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc
    23041 gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg
    23101 tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa
    23161 ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc
    23221 aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga
    23281 ttagccagga ttcaccgctg taagcagcgg gagtctgcgt cgcatcaggc aataagcgcc
    23341 ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac
    23401 cgccatcacg ataatggtga acggtacccc cagcttcaca aagtcactga agctgtaatt
    23461 tcccggacct aaaaccagtg tgttaaccgg tgaagaaacc ggtgtcataa aggcggcgga
    23521 tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc
    23581 agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat
    23641 aaccgccgac aagacaaaca aacaacccag catcatatgt ggcccgtaac caccgccaat
    23701 gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa
    23761 cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc
    23821 agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt
    23881 aggaatttca tctgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg
    23941 tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa
    24001 gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat
    24061 atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt
    24121 tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc
    24181 aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc
    24241 agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg
    24301 gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga
    24361 aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac
    24421 gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccaa tcattggcga
    24481 tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc
    24541 gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat
    24601 gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt
    24661 tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag
    24721 gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg
    24781 catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat
    24841 aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat
    24901 caacaaaacc aacattttga tttcactgtt gcccgcaact ttgaccagcc atgttcccat
    24961 tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag
    25021 gacaacgtta ggatcagaaa agccggaaaa tacttctggg actgtcagcg ttccgcttaa
    25081 tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa
    25141 caagacgatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta
    25201 tccttgttaa ttgaggggga tgacttgatt ctgccataaa aaagccccga cgagacgggg
    25261 ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca
    25321 gtaatttgat ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc
    25381 ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga
    25441 gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgtgc gcctaacaga
    25501 tacgcatcag gttctggttt tccgcgcttc actcgctcag cggtcacaaa cacctctggc
    25561 gcgggaagcc ccgctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca
    25621 atggcccacg gaatacctgc tttattcaaa tgattgagta aggcaattgc ccccggaagc
    25681 gcggtaatac cttcggtttc cgtggcctcg atttgctcca gacgcgtaaa ctcggcggca
    25741 atatcagcct cggatttgcc cgccataaaa tggcgcagag aggtgatcgc ctgtttaccg
    25801 tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac
    25861 gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct
    25921 ttgcaccgca cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa
    25981 atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat
    26041 tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac
    26101 attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag
    26161 gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg
    26221 caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc
    26281 gccaaactcg cgatccagtt cacgcatctg taatccataa ccacgctcaa tgattgtttg
    26341 caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct tgtactggtt
    26401 agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctatt
    26461 acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt
    26521 acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta
    26581 atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt
    26641 taccgcgaac ttcataaaac cagttgagga ttgcaggggg taatggcgtg acagaacgct
    26701 tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagggca gtggcaactg
    26761 ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg
    26821 gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg
    26881 caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa
    26941 aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag
    27001 attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc
    27061 catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga
    27121 gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc
    27181 aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc
    27241 ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta
    27301 taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga
    27361 aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt
    27421 tccacctgcc cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt
    27481 taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac
    27541 aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg
    27601 aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag
    27661 cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat
    27721 ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta
    27781 tgccggaaga gtcttacctc tacgccctgc cgtacaacct gtacaaagag cacggcatcc
    27841 gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc
    27901 tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg
    27961 tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag
    28021 gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg
    28081 acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagat tctggcctgc
    28141 tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag
    28201 aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct
    28261 acactgcgct gatggatggt cgtctggacg ctgttgtatt caccggtggt atcggtgaaa
    28321 atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg
    28381 atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta
    28441 cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc
    28501 gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca
    28561 aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg
    28621 aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg
    28681 cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga
    28741 tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa
    28801 aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat
    28861 cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt
    28921 cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa
    28981 tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg
    29041 tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat
    29101 cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga
    29161 gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga
    29221 atccagcccg ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg
    29281 tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac
    29341 tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt
    29401 ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg ttgccgcttg
    29461 cctggctgcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat
    29521 ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat
    29581 ggtgaacacc aacacctggc agacttctct gagcctgcag agcttcaacc tggaagttcc
    29641 agttgacgat cacgagcgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc
    29701 tgactggatc gattctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgccagc
    29761 gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga
    29821 aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac
    29881 ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga
    29941 actgggtgca ggcattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg
    30001 tctggtcgag ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga
    30061 agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc
    30121 cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc
    30181 accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt
    30241 ttacggtgac tgtgcgatca acccggatcc gaccgcagaa cagctggcag aaatcgcgat
    30301 tcagtccgct gattccgctg cagccttcgg tatcgaaccg cgcgttgcta tgctctccta
    30361 ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct
    30421 ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt
    30481 aatggctgac gttgcgaaat ctaaagcgcc gaactctccg gttgcaggtc gcgctaccgt
    30541 gttcatcttc ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc
    30601 cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc
    30661 ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc
    30721 acagcagcag taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa
    30781 tagtcactat ttccggtttt ttattctctt aatttgcatt aatcctttct gattatcttg
    30841 cttaactgcg ctgcatcaat gaattgcgcc atttcacttt gcatacttac cactttgttt
    30901 tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg
    30961 ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac
    31021 ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca
    31081 aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg
    31141 cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat
    31201 gaacttcccg tttgaagggt taacctcagg atcgaaatac gggacagccg ttggcatcat
    31261 catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga
    31321 taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc
    31381 gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc
    31441 ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt
    31501 cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg
    31561 tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat
    31621 cgtggtgtgg gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg
    31681 agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa
    31741 gcaggcggat gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct
    31801 gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga
    31861 aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg ttattttccg
    31921 ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat
    31981 gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg ttggtaatgg
    32041 tgaagcaggt gatgccagcg tgttaaatac tatcctcaac agcattgcca atgccattag
    32101 cggtctggac aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt
    32161 cttcgtgacg tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg
    32221 cgatctggtc ggtgttaacc gtcaggttac cgtgctggcg ttccagtttg gtgatggctt
    32281 cagtcacatc atttacccga cctcagcttc gttaatggcg acgctcggtg tttgccgggt
    32341 ggacttccgt aactggctga aggtgggcgc gaccctgctt ggactgctgt ttattatgtc
    32401 cagcgtcgta gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca
    32461 attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg
    32521 cttcatccac aacgctagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt
    32581 gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg
    32641 accgtgagag acgcagctga acaatgcgcc ccagacacgg caatttttat cttcgaaata
    32701 gaactgcccg tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg
    32761 acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa
    32821 catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc
    32881 atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgctcccg
    32941 gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc
    33001 caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt
    33061 tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag
    33121 tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt
    33181 cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgctactgg
    33241 cagatgggta tgaccgtaaa ccagcacatc gttctggttt aaagcaggca gattttccgg
    33301 gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca
    33361 cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg
    33421 cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta
    33481 accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca
    33541 ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg
    33601 aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg
    33661 atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct
    33721 ggacagacgc tcgctgccac tggaatgttg cataatccac caggcgttct ggcacctcat
    33781 cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt
    33841 caccaaataa attcggctga cccagtacta acaaatgttc tgccatcgcg aacagtttct
    33901 ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat
    33961 ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg caaccaggcc tgaatctgtc
    34021 gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa
    34081 atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt
    34141 gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggttgc aaatgttcac
    34201 cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc
    34261 aggcggataa cacataaggg gagaaacagt gggcatctga ccaaagcgtg atagcgggtt
    34321 tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc
    34381 actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga
    34441 tgattagaca accggagctg caatgatcga tctctatttc gccccgacac ccaatggcca
    34501 caaaattacg ctgtttctcg aagaagcagg tctggattat cgcttgataa aggtagacct
    34561 ggggaaaggc ggtcagtttc gcccggaatt tttgcgcatt tcgcctaaca acaaaattcc
    34621 ggcaattgtt gatcattctc cagccgatgg cggcgaaccg ctaagcctct ttgaatctgg
    34681 tgccattttg ttgtatctgg ctgagaaaac ggggctcttt ttgagtcatg aaacgcgtga
    34741 acgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg
    34801 gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta
    34861 tcaggttgaa actcagcgtc tgtaccatgt actgaacaag cggctggaaa actcgccctg
    34921 gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg
    34981 gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat
    35041 ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc
    35101 ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa
    35161 cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt acgtacgttt
    35221 atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc
    35281 cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc
    35341 accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa
    35401 ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg
    35461 gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac cttaagctgg
    35521 cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat
    35581 taattggtcg ccatttgatt ccacgtttgc tggagctggg ccatcaaatc acggtaatga
    35641 cgcgtaaccc gcagaaagcc agttccgttc tcggccctcg ggtgacacta tggcaagggc
    35701 ttgccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga
    35761 ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc cgctggaata
    35821 tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccaccgtcg gtactcattt
    35881 ccggctcggc aacgggctat tatggcgatt taggtgaagt ggtggttacc gaagaggaac
    35941 cgccgcataa cgaatttacc cataaactct gcacccgctg ggaagaaatt gcctgccggg
    36001 cgcaaagtga caaaacgcga gtgtgcctgc tgcgtaccgg cgtagtgctg gcaccggatg
    36061 gcggtattct cggtaaaatg ctgccgccgt ttcgtcttgg cctgggcggg ccgattggtt
    36121 ccggtcggca gtatctggcc tggattcata tcgatgatat ggtcaacggc attctctggc
    36181 tgctggataa cgagctgcgc gggccattta atatggtttc gccttacccg atacgcaatg
    36241 aacaatttgc ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg cgcgtccccg
    36301 caaccgccat tcggctgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg
    36361 cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ttggtacgat ttagaagagg
    36421 cgctggcgga tgtcgttcgc tgatgtggtt tactgcaaac atccgccagt taactcccgg
    36481 tgttacaggg ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg gtcagcgtca
    36541 ggatgacttc gtggtcaaat cctttttcca gcatctcctg agcaatacgc agagcttctt
    36601 cgtgtttgcc ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc atcagtttct
    36661 ccttttcttg tggtgcgcgt tctgttatct caccaataaa tgcacggaaa cgctgggcat
    36721 ctcccgtttg taatacataa ttaaacaggg cttttagctg tctgtcatta gtgttccctg
    36781 taactagcag cgaaacaatt tggtcgacta atcccaacag atcgcgctga cgaatatgtt
    36841 tctgaattaa ctccaacagc gccattttgc gatgttgcat aatctcgtca tccggcacca
    36901 cggtaatatc caccaacgga aaagccgatg aatatatttt acgggcaatc gcaggctcgg
    36961 caaattcatc aagccagcag agtgaataag gataaggact tctgcaacca tgataaaaca
    37021 gcatcgggat caccaatgga agctctttat agcccgcatc aagatggttt tgcatcgccg
    37081 caatggaata acgcatcatg cgaaaagcca ttaattcttc cggcttactt tggtgctcta
    37141 tcactacata aatataaccc actccctcct gcgttttcac agaccacaag aggtcggaat
    37201 aatattgccg caggtcttca tcaataaaac tgtttggttc cagtttaagc gtcgttaaat
    37261 cacacagttt gcgcagcggc gcggtaagat gaatatcaat aaaatcccgc gcggtgtctg
    37321 gatggcgtaa aaaagattta aataccgcat catgcggcgt ggaagttgtc gatattgtca
    37381 tggcgttccg tcacccctca aatttaagat gacgcgacaa taaccacgat taacacccaa
    37441 tgcagcaggc agtttttatc tttggacagc gaattcagaa gtatttttag cggactgcaa
    37501 cttttacatt tcagcggaag ctgactcgca aattcccctt gaaaatactc cggcagcgtt
    37561 agccaccgga gaggcaatta tttcagcgaa cctttaagga actgttgcag acgagggctt
    37621 tgcgggttgc caaataactg ctccggcgcg ccttcttcct ctattttccc ctgatggagg
    37681 aaaatgacgt gagtagaaac atgacgagca aagcccattt cgtgagtcac taccaccatg
    37741 gttttcccct cttctgccag ctgctgcata atgcgcaaca cttcgcctac cagttcagga
    37801 tcgagcgccg aggtaggttc atcaaacagt aaaacttccg gttccatcgc cagcgcccgc
    37861 gcgatagaaa cacgctgttg ctgaccgccg gaaagatgca ccggatattt cccctgtgca
    37921 cgttcgtcta tcccgacttt tgccagatac ttcaccgccc gctcgcgcgc ttcctgcttg
    37981 ctcaggccca acacctgaat cggcgcttcc atgacgtttt ccagcaccgt catatggctc
    38041 cagagattga agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt
    38101 ttatcggcga ctttgagttg accgtctttg tcgcgcacca gattgatcgt ctggccattg
    38161 accacgatcg ccccttcact cggtttttcg aggaagttaa tgcagcgcag aaaggtactt
    38221 ttccccgatc ccgacgatcc aatgatgctt attacatctc cggcattcgc ttgcagtgac
    38281 acccctttca gcacttcgtg ttcgccatag cgtttgtgca aatcgataac gtttaattta
    38341 ttctcggaca tcgtattctc agtgcgttga agaaggtttc acatgctgca accagcgttt
    38401 ttccgctctg cgaaagaggc tgatcaggac ataagagatg attaaataga gcaccgcggc
    38461 aatgccgaag gcagtaaaag gttgatacgt ggcggcgtta atatcgcggg ctattttcag
    38521 cagatccggc accgtggcag taaatgccaa cgcagtagag tgcagcatca ggatcacttc
    38581 gttgctgtat gccggtaacg caatacgcag cgcagaaggc aaaataatgc agcgatacat
    38641 tttaaaagtc gagaagccat aggctctggc ggcttcaatt tccccatgcg gaaccgaacg
    38701 gattgcccca gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac
    38761 ggtacagttc aggccactgc ggaaaaaggc gttaagaaac tcggtcccct taacaatctc
    38821 cagcgtatac atgccggaat agaacaccag caactgaaca tacagcggcg taccgcgaaa
    38881 aatataggta aataaccaga ttggaaactg gatgtattta ttactggaga cacgaccaat
    38941 cgccagaaac agcgccagga ctccgcctat cactaccgac aaaataagca gccacagggt
    39001 gatcgccaca ccagtaaagc gataaccgtc ggtccacagc agcggtttcc agtattcatg
    39061 taagatttcg atcacagatc agccctcttc acacccacgg agtagcggcg ctcaaggaac
    39121 agcagcacac cattggaaac ggtggtgaaa accaagtaaa tcacgccaca gacgatggcg
    39181 aaatagaacg gttcccaggt acttttgcct gccagctgcg tggctttgac cacatcttcc
    39241 aggccgagta acgaaaccag tgcggtagat ttgaggatca cctgccagtt gttgccaatg
    39301 cctggcaggg cgtaacgcat catcgccgga aacatgatcc gccgaaacac ttgcccacga
    39361 gtaaaaccga acgccgtcgc cgcctctata tgtcctttcg gcactgccat aaaagcacca
    39421 cggaacgttt cggtaaaata agcaccgtag ataaaaccga gagtgataat accagcgacc
    39481 atcggatcga tatcaatctg cccgacgccc atcgcctccg tcaccgtgtt tagcgcaatc
    39541 tgcaaaccgt agaaaatcag cagcatcaac actaagtccg gcacgccacg aatcagcgtg
    39601 gtgtaccctt cgaaaatcag ccccgaaagc cgattttgcg agagcttacc gccagcgcca
    39661 attaaaccga tgattacagc gagcactaca gagctgatag ccagctccag cgtgacgagc
    39721 gcaccctgta aaataacacc tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg
    39781 gtgaattacc gcctcttgtc tccctccggt tacccgaagg gagcgggctt tctttcaccg
    39841 atggcgatta accaccataa acatcaaaat cgaagtactt tttcgctaat ttctcgtaag
    39901 taccgtcagc tcgcatttcg gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt
    39961 ctttacgcag gcccatgccg gtccctacgc caaacagttt ttcatcttta acagacgggc
    40021 caccgaattt gtaatcttta ccgacaggtt gtttgaggaa accttcgctg gcagcgacct
    40081 catcctggaa cgcggcatca atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc
    40141 cctgatacga gacgatttca atgccttttg gtgcccagtg ttcgttaccg aacgtctcct
    40201 gggtggtgcc ctgcaatacg cctacccgtt tgcctttcag cgactcgact gtcggctgaa
    40261 tgtcagaatt tttcgccacc accagacgag aatcggcagc gtacagtttg tcggtgaagg
    40321 ctatttcttg ctgacgtttt tccgtaatgg aaagcgatga catgatggcg tcaatcttct
    40381 tcgcttttaa ggacgggatt aacgcatcca gcggattttc gacaaacgta cattgcgtat
    40441 tgatgcgttt gcataattcc tttgccagat cgatatcgaa gccaaccagt tcgccttgtg
    40501 aattttttga ttcaaatggc gcataggtcg ggtcggtacc gatgcggatt ttttgcggaa
    40561 tcgcagcaaa tgccgcagtt gcgctggaga aggccagaac cagagagagc gatagcacca
    40621 gttttttcat aatgtcctca actgacagtc tttttatagg gaatatttac aggtttgtta
    40681 cacttatcgt gccataaaat tgaccatcaa ggcaaaatat ctcgccccgt tagacgtatt
    40741 atttttcatt aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg caacaaacct
    40801 ggcgtgcacc atgatgatca ttccatcagg tacagcttcc ctgcgacgta tcagtcaccg
    40861 tagacattaa agtcaaaata ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc
    40921 tctccaagcg ccttattgaa agcagccgtc agttcagcat catctttacg tagccctaca
    40981 ccggtgccat caccgaagta ttttttgtct ttcactgatg agccagcaaa ggcgaagtct
    41041 ttaccagcag gttgcttgag gaatccttcg ctggcagcaa cttcatcctg cagtgcagca
    41101 tccagacgtc ctgcagccag atcggaatag accaaatcct ggttggcata ggccaccaca
    41161 tccacacctt tgctacgcca ggtctcgtta gcgtaagctt cctgggttga tccctgcagc
    41221 acaccaacat gtttaccttt cagtgaatcc agcgttggct gaatcggcga acctttggcg
    41281 gcaatcagac gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt
    41341 ttatcggtaa tggaaagcga cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg
    41401 atcagcgcgt caaagtcact ggcaacccag gtacatttca cctgcatccg tttgcacatc
    41461 tcgttaccga gatcgatatc aaagccaaca aaatcacctt tagcatcttt cgacgagaac
    41521 ggcgcatagg tggtatcggt tccgatacgt accgtctccg gtagcgccgc atagctggaa
    41581 gccgctgcgg agagaccgac taacaaagac agagcgagaa tcgacttctt catacataac
    41641 cctcaagtga aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc aggctatttc
    41701 atgcaggtct tatgccatct tgacgacaac agtaacattc aacgttaaat gtgttaataa
    41761 gacgttgcat tattgtcctg aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt
    41821 tcgattgaat ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa attgccctga
    41881 aacagggcaa cagcggagtt atgcgccctg ccagcgggca aagagatctt caggaagggt
    41941 tatcgcaaac tggtcaagaa cacgattaac cgtctgattt atcacatcat caagggattg
    42001 cgggcgatga taaaacgccg gaacgggagg cataatcacc gcaccgattt ctgctgcctg
    42061 agtcattaaa cgcagatgac ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg
    42121 gcgacgctct ttcagcacaa catctgccgc acgggtcagt aagccatcag tatagctatg
    42181 gacaatgccg gacagggttt tgattgaaca gggtaaaatc accatcccca gcgtctggaa
    42241 agaaccggaa gagatgctgg cggcaatatc gcgcgcatca tgcgtgacat cagctaatgc
    42301 ctgcacttcg cgcagggaaa aatccgtttc gagggataag gtctggcgcg ccgcctggct
    42361 catcaccaga tgcgtttcga tatctgtgac atcgcgcaga acctgtaata aacgcacgcc
    42421 ataaatcgcg ccgctggcac cgctgatgcc tacaatgagt cgtttcataa atagttgccc
    42481 tgtcagactt gcaggcagac tttgcaggat ttcggcggga gttgcaagtc agggtgccag
    42541 accggcaccc tgagcgaagg catcatcctt cgttatgcat ttcgagattt tccacttcgt
    42601 tctgacgttg cactgctttg gcgtcgtcat tacgtaacgt atcgaggaaa tcgaggtagc
    42661 cctgatcaac atctttggtg acgtagacgc cgttgaacac cgaacattca aactgctgaa
    42721 tatccgggtt ttcagcgcga acggcttcga tcagatcgtt cagatcctgg aaaatcaacc
    42781 cgtcagcacc gatgatctgg cgaatttcat ctacttcgcg accgtgagcg atcagttccg
    42841 tggcgctcgg catatcaata ccataaacgt tcgggaagcg aatttccggt gccgcagaag
    42901 cgaggtacac tttcttcgct ccggcttcgc gtgccatctc gataatctgc tcagaagtgg
    42961 tgccacggac gatggagtcg tcgaccagca ggacgttttt atcgcggaac tcggcgcggt
    43021 tggcgttcag tttacggcgc acggacttac gacgcagctg ctggcccggc atgatgaagg
    43081 tgcggccaac atagcggttt ttaacgaagc cctggcggta cggcttgccc agaatacgag
    43141 caatttccag cgcgatatca cacgaggttt ccgggatcgg gatcaccacg tcgatatcca
    43201 gatcttccca ttcgcgggca attttctcgc ccagtttcgt gcccatattc acacgcgcgc
    43261 tgtaaacgga aattttgtcg atgaacgagt ccgggcgagc aaagtataca tactcaaaca
    43321 ggcacggatt gctgaccgga ttgtcagcac attgacgggt aaacaactgc ccttcttcag
    43381 tgatgtaaat cgcttcgccc ggcgcgacgt cacgcaggaa atcaaatccc agcgtatcga
    43441 gcgcaacgct ttcggaagcg accatatatt ctgtacggtt ctcgtcaata tcacgttttc
    43501 ccagtaccag cggacgaata ccgtttggat cgcggaaagc aaccataccg tggccgataa
    43561 tcatcgccac acaagcatac gcgccgcgga ttaagcggtt tgtagcagcg atggcggcga
    43621 aaatgttgtc ggcttccagc gggtagtggc ggaagttgtc cagctcgctg gcgaagatat
    43681 taagcagaat ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt
    43741 ttttacgcaa ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta atgccatacg
    43801 gggagttaac gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc gtggggtaac
    43861 gcacatgacc aatgcccata ttgccctgca aacgctgcat atggcgagct tcaaatacat
    43921 cgctcaccag cccgttcgct ttacgcaaac ggaagcagtt attggcatct atggtgatga
    43981 tgccggcggc atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa atcgactggt
    44041 taaccggcat aacaccggcg ataccgacaa taccgcacat acgtcttttt cctcgttaag
    44101 ccacatctca gagcacttac gctctgggca agaaacttga cgagctttgc agataatcaa
    44161 aaaaccatct gatgataaaa ctgaactgcg ggatcagctg tgatttactc cagtcttcgc
    44221 ttttcgacac cccggtaaag gagtcgagaa agaagagaat ggcagcaaca atcaacacac
    44281 cgcgcaacgc accgaaacag acgcccagca cccgatcggt gcctgacaac cccgttttct
    44341 ccaccagctg gcctatcacg aagttcacga tagcaccaac gatcagggta gcgataaaca
    44401 gtaccgcgat ggcaatccca tttcgaacca gttcgtcttc aaagcccgta aaccagactg
    44461 acaggtaagt gtagtaatga ctggcaacaa agaaagcaca accccatgtc accagcgata
    44521 acgcttcacg aacaaagccg cggatcaggc taaccagaga ggaaaaagca atcaccgcga
    44581 ttatggcgta atcaatccag accatatgtg tcccacgatt ttacgccctg tcatcctgtt
    44641 cggggcgcat tctaacagaa aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca
    44701 ataaaaatgg cgctgaaaaa atattcaacg ccatcgactt tttatgcctt tgcggcatcg
    44761 gacaatgcgt gtcggatgcg gcgtaaacgc cttatccgac ctacggttct aaccctgcgt
    44821 aggcctgata agacgcgcca gcgtcgcatc aggcaacggc tgtcggatgc ggcgtgaatg
    44881 ccttatccga cctacggttc tacccctgcg taggcctgat aagacgcacc agcgtcgcat
    44941 caggcaacgg ctgtcggatg cggcgtgaac gccttatccg acctacggtt ctacccctgc
    45001 gtaggcctga taagacgcgc cagcgtcgca tcaggcaaga ccgtattaat tcggcgtata
    45061 gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg atcctttcag
    45121 cttatctttc gaggcatctg gcccaaccag aatacgggta attttaccct gcactggcgt
    45181 ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa tctcattcac
    45241 tttatcggca tttttcagcg cccccagttg cacaacatag gctttacccg tcggtgcagc
    45301 tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt caacctttgg
    45361 tttaggcggc tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa
    45421 ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac ggtgctgcgg catcacctgc
    45481 ccgcacctct tccgctgcgc cttccggcgg ttgcgtcggc aacgcctggg tggcggctgg
    45541 catcatatca ggctcatcgc gatcgcccgc ttttggcacc agcgggatag ccgcaaactc
    45601 atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc
    45661 caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg
    45721 cgtcaatcac ttccatgaca tgtgcgaccg tgtggaacga accacacacc agcacggtgt
    45781 cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta tcaaatgatt
    45841 tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg
    45901 gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt
    45961 tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc gttttcatac
    46021 gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg
    46081 actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg
    46141 cattttcact gacctccagc ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg
    46201 gttgcgggac aagcggcaac ggcaggtttt ccagcgtgcc gtgagcatcg ctaaacgccc
    46261 agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacctt
    46321 tttcctgcgc tacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt
    46381 tttcgctgcg aaagatgcct gctttctcgc gaccaatact ttcgcgatct ggccccagcc
    46441 agtcggtatg atccagcgca atgctggtta ctaccgcgac atcggcgtcg acaatattgg
    46501 ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt tgtgcctgtt
    46561 tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc
    46621 gtgccgattc aatctccgca aaagaggcag tgtgggccga ttccggcaat tcctgaccct
    46681 gcacacgtac gcgctcggta taacgcacca gatgcggcga actgtagacg cccactttgt
    46741 accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg ccattcgtac
    46801 ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc gcgacctggc
    46861 tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc
    46921 acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt
    46981 cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg
    47041 cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc gcttcaggat
    47101 tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg
    47161 gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg cgctggaatc
    47221 ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca aagccgatta
    47281 acgctttagg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc
    47341 ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca
    47401 gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc
    47461 caccagaggc ggagaagcag atcagtgggc agttatcttc cagcgcctgc tcaacggcac
    47521 gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg aactcgaatg
    47581 ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc gcatcttttt
    47641 cgccggtttc tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact
    47701 tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca
    47761 ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct
    47821 caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc
    47881 acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa
    47941 tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg
    48001 acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag
    48061 cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg
    48121 tgccgcagcg cgtttgtgac ggattatttc gatgacgccc ggcaaaatag aaaccacaat
    48181 aatcccgacg atcagcagct taaggttatc ctgaaccatc gggattgtac cgaagaaata
    48241 gcctgcgtag gtaaaaagca gtacccacaa cagtgcgccg atcacgttat aagcggcgaa
    48301 atgacggtac gacatgtggc ccattcccgc aacaaacggg gcgaacgttc tgacgatcgg
    48361 cacaaaacgg gcgagaataa tcgttttgcc gccatgtttc tcataaaact gatgggtttt
    48421 gtcgagataa ctgcgacgga aaattttcga attgggatta ctgaataact tttcaccgaa
    48481 caaccgcccg atagtgtaat tgaccgcgtc acccacaatc gcggcgatca gcatcagcac
    48541 caccatcata tggacgttga gatcgttggt ttccagtgat gccaacgctc cggcaacaaa
    48601 cagcaatgaa tcacccggta aaaacggcgt taccaccaga ccggtttcac agaacaaaat
    48661 taagaacaaa atggcataaa cccagacgcc gtactccgcg accagctctg ccagatgcac
    48721 gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg cctatgcctt
    48781 gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt
    48841 attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcaaaccggt
    48901 cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgccagcg
    48961 ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga
    49021 cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga
    49081 tatcaaccac cacataagga ccgtgacgcg tgacattaat atgcataacg ttgcgccacg
    49141 gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc
    49201 attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt
    49261 tactcagtac cgccgggcgc agccgatgat tgtagatgat atagcgataa cggcgagccg
    49321 tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt
    49381 caccaggtaa attcgcattt acgcccaacg tccacgcagc gtctttgcgc tgcgcggttg
    49441 tttcgaaatg caccacctgc ccggtaccgt gtacccctgc gtcagtacgc ccggcgcaga
    49501 agacggtgat gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac
    49561 tgcgaacttc gttctgccgt tgccagccgt aatacttact gccgtcgtac tcaatgccca
    49621 gcgcaatttt ataaactggc ggttgttgct ggtcggacat tagtacagat actcctgcac
    49681 cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac
    49741 cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc caacagaaag
    49801 atgcggcgta cccgaagcat cccctacctg agttgggaat tcgttctctt cagagagcac
    49861 aatatcttcg ccttgaacaa acgcatcacg cgcttcttct gctgccagtg gacgcagagc
    49921 ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga
    49981 aatcatcagt ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc
    50041 acgcacgcta ccttcgctat ccggcagtaa cggcagcatg ttgaacgcca gctgacgccc
    50101 gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttagcactct gccccgctaa
    50161 cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cgctgatacg
    50221 tgataaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc tggtcagact
    50281 gtccggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc
    50341 cggcaccacc agcggtacgt cgggttcgag agcaaacaat ccgctgctgt cgatcaccag
    50401 gcaacctgag ttggtcgctt cttcaaccca ggctgcggta gcttctttgc ctgcgacaaa
    50461 aaatgccagc tgcgcctgcg tccagtcgaa ttcagcagca tcctgcacgg tgattgtctt
    50521 accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc
    50581 aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc
    50641 gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc
    50701 tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg
    50761 gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac
    50821 agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt
    50881 atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac caaatgcacc
    50941 agccttttca gcgttggttg atcgagcggg ccatgcagcg taatgcgacc aaactctggc
    51001 gcgggcagta atgtatccag cgcaatgtgc tgttcatgcc caataaactt gctataggct
    51061 tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tatagcctgc aatatgcggc
    51121 gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc ttcccagaca
    51181 tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcagcgc ggtattatcg
    51241 acgactgcgc cacggcaggc gttaatcaga atcgctccgg gcttcaggtt acggatcagt
    51301 ttttcatccg ccagatgcag cgttttgtac ggcccgtctt tgaagagtgg cgtatggaaa
    51361 gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc ctcatcccca
    51421 cggtcagcgc gaggcggatc gcaaagcaag gttttaatcc ctaacgcttc cagtcgcgcc
    51481 tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc gtgcagtgaa
    51541 aatccatcgc gttcggcaag catcagtagt gaggagagaa catattccac caccgcaatc
    51601 gcattacagc caggtgcagc ggaaaaacca attcccgcct tctttaacca tgcttcatcg
    51661 acatggtctg tccccgcagt ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa
    51721 gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg
    51781 acggggattg gacgcccggg aaccgcgatc acctcaccca aacggctaaa taagtcgcgg
    51841 gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag
    51901 agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt
    51961 ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac caccaggtca
    52021 gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc
    52081 gctggaaagc ttaatgacga aagtgacctc actgacgcaa cagcaaagag cagaactgtg
    52141 ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt
    52201 ccctgccgct gagcataatc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc
    52261 ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc gtcaggcggt
    52321 cacggattat atccgtcata actttggtca gacgccgctg aatcagctct caccggagca
    52381 attaaaaacc attctcaccc tgttgcagga aggcaagatg gttattccgc aaccgcagca
    52441 gcgcgaggcg accgatcgtc ctctattacc ggcggagcac aatgcgctca aacagctggt
    52501 gaccaaactt gcggcggcaa cgggggagcc cagcaaacag atctggcaat cgatgctgga
    52561 actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac
    52621 ctggctgcag gcgcgtcaga cgctaagcca gcaaaatacg ccgacgctgg aatcactaca
    52681 aatgacgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg catatatcca
    52741 gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct
    52801 taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct
    52861 taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg
    52921 gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttaac cccgacgaaa
    52981 cgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga
    53041 agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc cgagggcgat
    53101 atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg ggacacgttt
    53161 aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc
    53221 gcccgctaat gcgacccacg cacccggggc ttgccagagc agcaacaagc ccaccgtttc
    53281 tacaagcaga gagacgatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa
    53341 catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc
    53401 ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc
    53461 ttgtagtgcc agccctaacc ctggtttcca gataagcccg acaacgctcc acagcgatgg
    53521 acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc acgccagtaa
    53581 gggtaatgcc attgtggtga gcgccagtgc ggcaaaaccg taatggctat gaatcaacag
    53641 gccaagcggt gcaccaacag cgagggcacc gtaaatcgcc attccgttcc atgacattac
    53701 tttgccagag tgttttggcc ctacgatgcc taatccccag gtcagagcgc ctgtcagtaa
    53761 ctggctttca ccaaagccaa gaatcaaacg cccgacgacc aacagggcaa atttgaacgg
    53821 tgcggagacg ggcaaaatcg ccgccagcag caacgcgccc cccgccagac cacaagctaa
    53881 catcccctga agcgccgaac gttttgcgcc atattgatcg gccagtcgcc cggcgtaacc
    53941 acgcgtcagc accgtagcca gaaactgaat gccgacggca atgccgacca tggtattgcc
    54001 atagcccaat tcatgatgaa caaacaacgg gataaccggc aacggcaacc ctacggtcat
    54061 gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt
    54121 ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat
    54181 ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggaatta acgcgtacgt
    54241 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    54301 gatgtgaaaa agggaaccat cgggttccct ttggcgttag tgccggatgc cgcattggcg
    54361 cgtaacgtcg gatgcgacgc tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc
    54421 gacctactcc gactactccg gcctactgcg aattaatctt tcagcttgcg cattaccagc
    54481 gtggcgttgg tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg
    54541 gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg
    54601 ctcggggcga taaagccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca
    54661 gcagcgccca gagagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg
    54721 ccgaacactt cacggatagc tgccagctcc ttcacgtcgc caaccggagt cgaagtgccg
    54781 tgggagttca ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt
    54841 actgcgcctt cgccagacgg agcaaccatg tctgcgccat cagacgttgc gccgtagcca
    54901 acgatttcag catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccacc
    54961 accataccgc cgccgccagc gataacgaaa ccgtcacggt gagcatcgta agtacgggag
    55021 gctttttccg gggtgtcgtt gtatttagta gacagcgcac ccattgcgtc gaattcgcaa
    55081 gccatttccc agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt
    55141 tggatctgct ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg
    55201 gagtagttaa cgccatgaat tttaaacggg gtggcgaggc aggcagaaac gccggatgcc
    55261 atcgctttgg tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg
    55321 tcagcgccga acacctggaa acgcggggag ccgccgccgg aacctgcaat caggccaacg
    55381 cgcgggttat tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata
    55441 gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg
    55501 ccagtggtat ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc
    55561 tcctgagaga aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg
    55621 ttattaccga tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac
    55681 ctctgtaagt cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga
    55741 acaagtccga tcagccaatt aatagagaaa tttgcgcagc cttacacaca tcgctaagat
    55801 cgagccaccg cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa
    55861 cctcgaattt aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc
    55921 caacgataac gggctggaag agacgcgtta tgtttttctc ggaggtaacc aattagaggc
    55981 acgctttcct gagcatctac atcctctgtt tgtggtagca gagagcggct tcggcaccgg
    56041 attaaacttc ctgacgctat ggcaggcatt tgatcagttt cgcgaagcgc atccgcaagc
    56101 gcaattacaa cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt
    56161 agccttagcg catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca
    56221 gtggccaatg cccttgcccg gttgccatcg tttattactt gatgaaggcc gcgtgacgct
    56281 ggatttatgg tttggcgata ttaatgaact gatcagccaa ctggacgatt ctctaaatca
    56341 aaaagtagat gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac
    56401 gcaaaatctg tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt
    56461 tacgtctgcc ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg
    56521 taagggcttt ggacgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact
    56581 cccctgctcc acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat
    56641 cggcggtggt attgccagcg cattgttgtc gctggcgcta ttgcgtcgcg gctggcaagt
    56701 aacgctttat tgcgcggatg aagtccccgc gctgggtgct tccggcaatc gccagggggc
    56761 gttgtatccg ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgggtt
    56821 tacttttgct cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg
    56881 cgtcacgcag ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgctgtcaat
    56941 ggatttaccc gaagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg
    57001 cgttgcgaca aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga
    57061 actgacccgt aatgtgctga aactggcgca acagcagggt ttgcagattt attatcaata
    57121 tcagttacag gatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca
    57181 gcaagcaaca cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca
    57241 aacatcatct ctgccagtat attcggttgc cggtcaggtc agccatattc cgacgacacc
    57301 ggaactggca aaactgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc
    57361 ggcgaatcag catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta
    57421 cagtgaggac gatcagcagc agaatcgcca gcggttgatt gattgtttcc cacaggcaca
    57481 gtgggcaaaa gaggttgata tcagtgagaa agaggcgcgc tgcggtgtgc gttgtgccac
    57541 ccgcgatcat ctgccaatgg taggtaatgt tcccgattat gaggcaacac tcgtggaata
    57601 tgcgtcgctg gtggagcaga aagataaggc ggtaagcgcg ccggtttatg acgatctctt
    57661 tatgcttgat gctttgggtt cgcgtggttt gtgttctgcc ccactgtgcg cagagattct
    57721 ggcggcacag atgagtgaag aaccgattcc gatggatgcc agtacgctgg cggcgttaaa
    57781 tccgaatcgg ttatgggtgc ggaaattgtt gaagggtaaa gcagttaagg ctgggtaacc
    57841 agctttggca ttgtttgtcg gatgcggcgt gaacgcctta tccgacctaa gtgtgacgtg
    57901 taggcctgat aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt
    57961 actgctgtga cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac
    58021 gcggcgacaa ttcaccagca ccaatggctt tttccagact ctggctaacg gtcgtatgca
    58081 ccgcctgagc ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc
    58141 cacgcaaata cccactggca aacaactcat catcactggc atggtcaacc ataccgtcga
    58201 ttaatgccag aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt
    58261 ggcgctggct tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg
    58321 tgtgataata attttgtagt gctttaatga aacgtgccgg gcgttccgga atgccatttg
    58381 ttagataatc catcacctgc gcatgtactc gccgttgaaa caccacgcgg tccggttcga
    58441 agtcgccttc cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaaca
    58501 accagtcgag cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtttgggcat
    58561 cgcgtccgtc cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg
    58621 caatacacca gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga
    58681 ttcggttata cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa
    58741 tcagacgggt attaaaatca tcggcaaagc agctattaaa aatttcaatc aactgctcat
    58801 agtggtgtgt actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct
    58861 atcgtaaagt aatttggcac tcatcaccgc ggagacgata acaatcatcg ggcggatcag
    58921 cttttgtcct ttgctcaaca ccagtcgtga acccatgcgc gcgccgagga actgccccac
    58981 cagcatcaca aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcaaacc
    59041 accgatgttt gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa
    59101 tccgcacagc gtaacaaagg ccagcgcgta aaacgatccg gctgccggac caaagaatcc
    59161 gtcataaaaa ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg
    59221 ctggcgatct tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac
    59281 cagaatgggc aaaatctgcc gtaagacatc agcctgaacg tactgtacca gcagcgcgcc
    59341 gctcattgag ccgacaaagg tcatggcgat attgagtttc tgatcgctta aactaaccac
    59401 tttgcggcga ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt
    59461 ggttgccagc gcattagcgg gagacatccc cgccgccatc aatgccggaa tggtgagtaa
    59521 cccaccgcca ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac
    59581 tcccagcaac agcggggaaa ccataaacag gctattaaac gtttccatta gatcacgtgc
    59641 tcatccagta gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt
    59701 gttgttcccg gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct
    59761 ggtggcggta aaggttgatc ttcacactcc agactatcgg caggacaacg taatcgtaca
    59821 tgcatatgcg cacgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg
    59881 cccgcatcaa ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct
    59941 ttgtcctggg cggcgagttt gatcaagctg aaaatttccg gcttccacag cgtggggaca
    60001 acgcgtttac cgtcgcggga tactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag
    60061 gtccagcgag ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg
    60121 gcatgaccgc cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta
    60181 cccatgccca gattgctcac ctggctactc agacgctgga taaacatcac caggtccggg
    60241 tgaccgaaat agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc
    60301 agcgtgtcag cgccgacgat acagccatta gaaaaactgc ctatcgattg tgcgctaccc
    60361 ggcacaggtt gggttatttt ttgccacggc gtcgctgcca ggctgacgct actggcaagc
    60421 agggccagta gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc
    60481 acatcggcat tttgcgcccg ctgccgtaac aggtgatcca ttaatacgat cgccagcatc
    60541 gcttctgcga tcggcacggc gcggatcccg acacagggat cgtgacggcc tttggtgatc
    60601 atctcaactt cttcgccaaa gcggttaatg gtgcgccccg gcacggtaat gctggaggtc
    60661 ggtttcagcg ccatatgggc aatgatttgc tgcccgctgc tgataccgcc aagaatgccg
    60721 cccgcatggt tgctctggaa accgtctttg gtgatttcgt cgcgattctg actgccgcgc
    60781 agcgccacta cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc
    60841 agcgcatggg cgatgtcggc atccaggcgg tcaaagaccg gctcaccaag cccggcagga
    60901 acgccactgg caacaacggt gactttcgcg ccaatagagt cgccctcttt tttcagcgcg
    60961 cgcatcagtt catctaacgc gtcgattttg tccgggtccg ggcagaagaa cggattttgt
    61021 tcaacctgcg accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg
    61081 cggatttcaa taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg
    61141 cgcatggcgg tttcgcgggc ggaagaacgt ccgccgccgc gataatcacg cagaccgtat
    61201 ttttgttcgt aggtgtaatc agcatggcct gggcggaaaa cgtctttaat tgcgctgtaa
    61261 tcctgagaac gctgatcggt attttcgatc aacaaaccaa tgctggtgcc ggtagtaaca
    61321 ccttcaaaaa caccggagag aattttgacc tgatccggct cacgacgctg ggtggtatag
    61381 cgcgatgtcc cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga
    61441 atgcctggcg gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag
    61501 gtggttacgc gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg
    61561 tgtttgcgtg tttacttaat ctttataaat cgcgaaatgt tcacgggccg ccagcagctg
    61621 ctctttggtg agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg
    61681 aacatccgga tattgttcca taagatgtac catgctgttg ccgacttcac aaatcagcac
    61741 accatcatca gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc
    61801 gtcagtgcca gatgccaggc ccagttctgg ctcgtgacgg tattcgtttg gcaggtcgga
    61861 catatcttcc gcatcgacat acggcgggtt agtgacaatc aggtcgtact gcactttcgg
    61921 caaatcacgg aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc
    61981 gatgttctgt tcggcaaccg ccagcgcgtc gggagagata tctaccgcat cgacttccgc
    62041 ttccgggaag gcataagcac aggcaatggc aatgcagccg ctaccggtac acatatctaa
    62101 aatatgctgc ggttgcttgc tgataagtcc ggcaaattta ttgttgatca attcaccaat
    62161 cggcgagcgc ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc
    62221 tttattggtc aggtaagcca ccggaatgcg ttcgttgacg cggcggatca cgcgctcaac
    62281 aatacggtgt ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg gaatatccag
    62341 cggcaggtag agcgacggca acaccaattg taccgcttca tcccacgggt tatcggtgcc
    62401 gtgaccgtac cagatatttg ccgcgctgaa gcggctaacc gaccagcgca acatgtcctg
    62461 aatggtttgc agctcattaa ctgcttcatc aacgaaaatt ttatccacgt attcctccag
    62521 ggcatgatcg caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt
    62581 cacgcgcggt gagtgaggaa aaatacgttt aaaacgatcg attgcgctac gagtcgggta
    62641 aactgtagga aaattagaaa tagagacgca taaatgaaaa agaaaacaac acttagcgag
    62701 gaggaccagg ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacc
    62761 attgtccatc ggccacagcg taaaaaagtc agcgaagtgc cggtgaaacg cttgatccag
    62821 gagcaggctg atgccagcca ttatttctca gatgagtttc agccgttact gaataccgaa
    62881 ggcccagtga aatatgtccg cccggatgtt agtcattttg aagcgaagaa gttgcgacgc
    62941 ggtgattatt cgccggaatt gtttttggat ttacacggtc tgacgcagct acaggccaag
    63001 caggaactgg gggcgttgat tgccgcctgc cgccgtgaac atgtgttttg cgcttgcgta
    63061 atgcacgggc acgggaagca tattttaaaa cagcaaacgc cgctgtggct ggcacaacat
    63121 ccgcatgtaa tggcatttca ccaggcgccg aaagagtacg gcggagatgc agcgttgtta
    63181 gtgttgattg aagtggaaga gtggctaccg ccggagttgc catgaatatt agggggcgga
    63241 tgcgttttgc catttcgccc tgaatgctgg gaccaaatgc cggatgcggg gtaaacgcct
    63301 tatccggcta cgaacactgt cgaggctaat aatattaatt aaagttcatt aagaatatat
    63361 tgaaagcgac atgtagcatc aaaactttgc cgttcactag tatcaacacg cacctgcatg
    63421 tcatggagtt gtttatcagt tacaggatgt cggtttgccg ttacgttcac tttattgtta
    63481 tcacaaccct ggacactaaa cgtcattaat tgtgatacca ggcgacaata ggctccacgg
    63541 tcatcactga agtcactgcc atcacataat cgcccatctt tcattaagaa tgtcggcaac
    63601 gatttctcaa taaccccccc cgtagattgc aggcttaatg atttattatg aacaacggtg
    63661 ttttcatccc aggaaatatc atagcttgct actggctctg ttggacaatt tgattggtgc
    63721 gcattcggcc cctgataata atatggcccc gcctgcggag gccttgctgt gctataaatt
    63781 gatgttgtca ccacccaagg gcaaccatgg gttgtacccg taacatgtac atttacagga
    63841 agtctatgac aaacccaacc agaggtcata ccgggacaat aagaatcatc tactatcggt
    63901 gcccgccctg gcggaattgt tatgtctggg gggacacccg gtccagtcca ggttaagtta
    63961 ggcacaactc ctgcctgtac cataatcccc ccttgtgaat atgtcccata atatgcccct
    64021 tcaggggtta acacatctat tttataaacg atagatgttg ttagcccact gccataagat
    64081 gcgccataag tctgaacatt attaaatgta acgtctgctt gtacagaaga attaagcccg
    64141 aacaataaca taccaaataa aatcactttt attttcattt agatatactc aaaaattgcc
    64201 gtcaggctgg cagtaaattc accagaagtg acttcacgcc cagatttcgc ctgcaaccaa
    64261 gccttaaagt taagattatt agcaccgtta ctcaggttaa atattgcccc tgccgaattg
    64321 ttgatgggga cctgtatccc atcaactgtt tccatgccaa tgctcacacc ttgcgcaccc
    64381 gatgatgcat ccaaagccag aaaccctggc aaatcgctat cttcggtacc ggttaacgta
    64441 actctaactt tgtaaccaac aggccctttg caatctttca attgaaaaac gaccggcaca
    64501 cgatcagatt gttcccgtga cattaaatct ctgctgctga tacttgggaa atggacttca
    64561 gccagcactt gtccttgtac taccagggta caagatttac ttaataaatt tccgtagaag
    64621 tgtaaattat tgtctgcacg cgctgtgcta tttcctgcca gtaacagcat tactatgagt
    64681 gcgaaccagg ttttcatctt cattgatatt ccacccgtaa tgtggccgaa gctgaaaacg
    64741 ttccgtctgt taatacagca tctaccgcct tcacggggac agcctctaat tgtggtttcg
    64801 tatcggcgtt aatttgtatc ggtgtattta ctctaaatgg ctgaccattt tgccgaagtt
    64861 taatccctaa tcctgttaca tcagtttcga ttgcagcatc atcataactt gtttgtgtgc
    64921 ctatccatgt caatgtcata tcccaggaac taccgggtgt aacagcatca catttaatat
    64981 cgtagggcac gacctctcgc ccattatttc catcaatgct atcgataatt acatccctga
    65041 actccacctc gatagtgttt ccatcactaa tagtacatgc cggaggcaat acaagagttc
    65101 ctttgaaggt gatatccggt gatgcattca caataccgct gctcatacag agcaataaat
    65161 acaagagtat tttcattgat aatccaccac cattgttaaa ctcccactaa aatcggcccc
    65221 tgaaagttgt gcaccacttt gttttaccgg gactgcatta agtttcggct gtgatgtgat
    65281 attgaaaggg gtccagttgt tttctccaat ggagaatagt gagttgttat ctgcattctc
    65341 gattcgaata ccaaatcctg gaacccctac atcaacgact ttttctccat taattgtggt
    65401 ggtgtttcct ctcaactgca tacgtaaatc atcagctttt cggtcagggc atgacaacgt
    65461 atatcctgca tctttacgat aattactacc attgatatcg ttgatattca cagtctgaaa
    65521 atctactgaa ctccccgtta cgctacatgg cggaggagga tctacatgta cctgtaaaat
    65581 gagatcaaca ggttttgaat ttgcaaggat aatgttatat atcccactaa cgcccagcag
    65641 taatataccc acccccacaa caaaaatttg tctcatgtcg ctctcccggc atcagccttt
    65701 gcggctctgc tctacatcca cctggcaggt atcaccgcca catttaaaat acagcggcat
    65761 ccgtgcgcca tagtcgttga cgtaagacag caccgggacg ctatccattt tgacttttaa
    65821 cggcacagag gtttttggcg atatcaccat gggggaaaat cccgctgccg gattgccatt
    65881 tttctgcgtg ctggcgttac tgataaccac gtagtacggc gtaggattat tgacggtaaa
    65941 ctcctgaccg ctgcgggtta aggtcacctt atgctgccac gggtttttca tgctgacgtt
    66001 ttccagcgcc tttggtcgcc agaacagctt gatgcgcgtt tgcagcgcta tctgtaacgt
    66061 gttgggttta ttcgatttcg gcggaatttc acgcacgttg aagtagaaca cgctctcgcg
    66121 gtcggcaggc agcttgttga tatccggcat cccctgcacc ttcacctggc cgttcatcat
    66181 ggaatcgatg cgctgcaccg gcggcaggac cgtcagtggc gagctaatcc tgttaccctg
    66241 ttcatcttcc atccagcttt gtgccagata cggcattttc gggtcgttat tacgcagcgt
    66301 gacgctgatt gatttatcac tctcattaaa aatcaggcgt gtacggtctg gcgtgacaga
    66361 ggcctgtgct gacaggctgg cagtggttag tagcaatgcc agcaacattg tggatttttt
    66421 ggcagagcaa aatagattcg tcatggatca ttccttaatc acgttattaa ttggcggata
    66481 ccgataccgg tgcttccgtt ggcatcacct gctgggtctg ctcctggatc atcggcttaa
    66541 tttcatgagg catcgaagga gatatcgccc ctgtttgctg gcatggtaat aacaggccat
    66601 tgaacagatc ggctggcagc gggtcaggca gatgaatatc gcagtgggcc acaccgcccc
    66661 atgaaacgat catatgctcg ccaggtttga cgcccgcgag gtagacgttg ccgtcatcgt
    66721 caaccagacc gacgttctgc gcgctgtcgt ttttcacttc cgcgccaaac ggcggataac
    66781 tgccatcttg cagacgcagc accgccatcg ctttttgccc gctgatcacc gaaaacttac
    66841 ggtagccaat ggctccttcg gtaagcgtcg cctgcaccac ggactgggtg gcttcggcgt
    66901 tttccggcag gttgttaagg tcgatatacg cctggttgcg gtagtagttg ttgacgtcgg
    66961 ccaccacggc cttaccgaac atattggtgt aaaccgccgc accgtttcct tccaccggaa
    67021 caccggccac gccatcggca tcaatcagca ggcgcgtacc gcccatattc tgggtacggt
    67081 ggagcgcgcc accctgcgcg gtcagcgtcg cgccgccctg taaggaaata cctgccgagg
    67141 tgtactgtcc ttcatgatag ttggcgctga gatcgacctg cgccagcgat ccgtcgtggc
    67201 tgtaataacc gtcaacgctg gagtgattgt cgctggtgcc tacgttcaac tggtaatggg
    67261 ttgcgtcatc gacacggctg aaatacccca cctggctgct gtccgaaccg ctgccgtagt
    67321 tgccgttata gctgatggtg ctgctgtcac cccacggcat gctgagcgat atgtaaacac
    67381 ctttatcgga ctggttgtca tactcgtagc ggtatccggt catggaaatg ctcatgttgc
    67441 ggatactgcc caggttgaag tagtgcgaga gcatgatgtt gtagttagtc tgttcgtcgc
    67501 gatcccagta ggtatggcgg gtgtagttga gataaacaga tacaccggca tccctgaagt
    67561 tctggttgta ggtggcggtg tacatctctt tgtcgttgcc ggtgcgcacc atttcgctgt
    67621 cgctggcatc gagatactcg ctcatggtca tgaagttctc ttccgagaag cggtatccgg
    67681 caaaagtgac gcggctgttc agttcatcga agtctttgga atagctcacg cgaaacgagt
    67741 taccgtccag tgaaccttta ccgtaggcag tttctttatc caggcgggta tgcgagtggg
    67801 tgacatcaaa ggccaccgca ccaaacacag acagatcgcg accgacgcca agcgccgccg
    67861 actgatagtg ttcatctgcc agcgccccgc cgtatagcga ccagccgttg gcaatccccc
    67921 aggaagcttc gccgccggag aagaaaccgc cttccacgtg gtgcccccac tcctgcgggc
    67981 ggcccatcat cagtttatag cgcacctggc cggggcgggt caggaacggc attgacgcag
    68041 tgttaatgtc atattcctgc acctgaccgt tctgctcttc aatgcggata tgcagcgtac
    68101 cggagacgga atcgccaaga tcctggatac ggaacggccc cgccggaacc tgagtttcgt
    68161 aaatcacgcg gcccatctgg ctgacggtca ctttcgcggt ggtgtgcgcc acgccggaga
    68221 tatccggcgc atagccgcgc agattcggcg gcaacatttg atcgtcggtg ctgatactgc
    68281 cacccacgta gttaaagccg tcgaaaatat cagaattcag gtagtcttca ccaaggccaa
    68341 gtttggcttt tagcgacggt aaggcccgcc aggcgtagta gcggctccac tcccagtttt
    68401 tttgcgtgtc atcaccgttg ataacatcgt catcattgct tttactatgc aaataatcag
    68461 tctgccagtc ggcacgaaga cgccatgcgc caaggttcac cccaaccgtc ccgttaccgc
    68521 taatttcatt actgtcatcc ccgccatttt cttcatgtcg tgtctgggcg gtaatactgt
    68581 agtcagcaat taaaccagat ataccgtcat cccagcgtga aggcggatcc cagttgatgt
    68641 cggtatattc aaggtaagcc tggggtaatg aaatgactaa cgctgactga ctcaggtcag
    68701 ctttaatatc aatgccttcc agttgaccgg gtttcaggca tttgccgtcg tggatccatt
    68761 gcaggttttt tgccacatcc tcttttaagc caaactgcgc gaccagttca ggcgtcaggc
    68821 aggcataggt tttactggca tcgttctcag aggcgtacca gtaaatatcg tattcttccg
    68881 tcagcggctg tttatttagt tgaacctgta aattgtattt cccaggttcg acataaccct
    68941 ggctggaaaa tcgcttcaga tcgatttttg tgtcgccttt taactccaga aaacgggaat
    69001 caaactgaat ttcatcttca gcccaggcat tgacataact cccggacatt gccaatgcaa
    69061 cgcagcaggg aagtacccgc agtcggaaaa gagaatggtc aggcatacac gtatccatcc
    69121 gtaattatta taatttgcgc gaattccatt taaaaacccc atagcccgaa agctatgggg
    69181 gtatcactta gagataagta atctggaagg tcgtgttggc ttcaaaatta cctaaatctg
    69241 gcgcatcagc agcgcccacc agccaggctt taaagtccag cgtctgcttc gctttgcctt
    69301 tgttggtcgc cgtatcgttt acgattttct gttcgatacc cgcgccgctt ttataagagg
    69361 ttccctgagc gtcaccaatg gccaggctga cattgttaaa tgccgcacca gtatcggtat
    69421 tgtaaatggt gtagtaattg ccgctgttgg tagaagacgc gttaccggtg aaggtagtgg
    69481 tcatcgttgt ctgggtgtca aacacacagt cttgcagatg gatctggaaa gctttcggtg
    69541 tagaagtccc gttgtttttc aggatccctg aaccaatgtc acccatatca acttccagtt
    69601 tgtgatcatc aggaacgatg gagcacgggg aatcttcaat ggtgccgtag aaacgtgctg
    69661 taccattgtt gccagcggcg attgttgaag tagaagcgaa cgcacccatt accattgttg
    69721 cagcaatagc tgtttttaca aacttactca t
//
LOCUS       DAZGCQ010000012        66114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000012 DAZGCQ010000000
VERSION     DAZGCQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.012"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(184..260)
                     /locus_tag="ACHK07_000856"
     tRNA            complement(184..260)
                     /locus_tag="ACHK07_000856"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Arg,seq:acg)
     gene            complement(264..356)
                     /locus_tag="ACHK07_000857"
     tRNA            complement(264..356)
                     /locus_tag="ACHK07_000857"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Ser,seq:gct)
     gene            complement(672..857)
                     /gene="csrA"
                     /locus_tag="ACHK07_000858"
     CDS             complement(672..857)
                     /gene="csrA"
                     /locus_tag="ACHK07_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO8101231.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1092..3722)
                     /gene="alaS"
                     /locus_tag="ACHK07_000859"
     CDS             complement(1092..3722)
                     /gene="alaS"
                     /locus_tag="ACHK07_000859"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO8101232.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3850..4350)
                     /gene="recX"
                     /locus_tag="ACHK07_000860"
     CDS             complement(3850..4350)
                     /gene="recX"
                     /locus_tag="ACHK07_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO8101233.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATEEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLSTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4587..5648)
                     /gene="recA"
                     /locus_tag="ACHK07_000861"
     CDS             complement(4587..5648)
                     /gene="recA"
                     /locus_tag="ACHK07_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO8101234.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5728..6225)
                     /gene="pncC"
                     /locus_tag="ACHK07_000862"
     CDS             complement(5728..6225)
                     /gene="pncC"
                     /locus_tag="ACHK07_000862"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO8101235.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6370..7455)
                     /gene="mltB"
                     /locus_tag="ACHK07_000863"
     CDS             complement(6370..7455)
                     /gene="mltB"
                     /locus_tag="ACHK07_000863"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO8101236.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7711..8274
                     /gene="srlA"
                     /locus_tag="ACHK07_000864"
                     /gene_synonym="gutA"
     CDS             7711..8274
                     /gene="srlA"
                     /locus_tag="ACHK07_000864"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO8101237.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8271..9230
                     /gene="srlE"
                     /locus_tag="ACHK07_000865"
                     /gene_synonym="gutE"
     CDS             8271..9230
                     /gene="srlE"
                     /locus_tag="ACHK07_000865"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO8101238.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLVQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9241..9612
                     /gene="srlB"
                     /locus_tag="ACHK07_000866"
                     /gene_synonym="gutB"
     CDS             9241..9612
                     /gene="srlB"
                     /locus_tag="ACHK07_000866"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO8101239.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9616..10395
                     /gene="srlD"
                     /locus_tag="ACHK07_000867"
     CDS             9616..10395
                     /gene="srlD"
                     /locus_tag="ACHK07_000867"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO8101240.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10501..10860
                     /gene="gutM"
                     /locus_tag="ACHK07_000868"
     CDS             10501..10860
                     /gene="gutM"
                     /locus_tag="ACHK07_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO8101241.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10927..11700
                     /gene="srlR"
                     /locus_tag="ACHK07_000869"
                     /gene_synonym="gutR"
     CDS             10927..11700
                     /gene="srlR"
                     /locus_tag="ACHK07_000869"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO8101242.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11693..12658
                     /gene="gutQ"
                     /locus_tag="ACHK07_000870"
     CDS             11693..12658
                     /gene="gutQ"
                     /locus_tag="ACHK07_000870"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO8101243.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(12655..14169)
                     /gene="norR"
                     /locus_tag="ACHK07_000871"
     CDS             complement(12655..14169)
                     /gene="norR"
                     /locus_tag="ACHK07_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO8101244.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAARFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14356..15795
                     /gene="norV"
                     /locus_tag="ACHK07_000872"
     CDS             14356..15795
                     /gene="norV"
                     /locus_tag="ACHK07_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO8101245.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15792..16925
                     /gene="norW"
                     /locus_tag="ACHK07_000873"
     CDS             15792..16925
                     /gene="norW"
                     /locus_tag="ACHK07_000873"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO8101246.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVMNIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17151..19403)
                     /gene="hypF"
                     /locus_tag="ACHK07_000874"
     CDS             complement(17151..19403)
                     /gene="hypF"
                     /locus_tag="ACHK07_000874"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO8101247.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLTTFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19556..20083)
                     /gene="hydN"
                     /locus_tag="ACHK07_000875"
     CDS             complement(19556..20083)
                     /gene="hydN"
                     /locus_tag="ACHK07_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO8101248.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20233..21243)
                     /gene="ascG"
                     /locus_tag="ACHK07_000876"
     CDS             complement(20233..21243)
                     /gene="ascG"
                     /locus_tag="ACHK07_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO8101249.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVMGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21503..22960
                     /gene="ascF"
                     /locus_tag="ACHK07_000877"
     CDS             21503..22960
                     /gene="ascF"
                     /locus_tag="ACHK07_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO8101250.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAISGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22969..24393
                     /gene="ascB"
                     /locus_tag="ACHK07_000878"
     CDS             22969..24393
                     /gene="ascB"
                     /locus_tag="ACHK07_000878"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO8101251.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24552..25022)
                     /gene="hycI"
                     /locus_tag="ACHK07_000879"
     CDS             complement(24552..25022)
                     /gene="hycI"
                     /locus_tag="ACHK07_000879"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO8101252.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(25015..25425)
                     /gene="hycH"
                     /locus_tag="ACHK07_000880"
     CDS             complement(25015..25425)
                     /gene="hycH"
                     /locus_tag="ACHK07_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO8101253.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25422..26189)
                     /gene="hycG"
                     /locus_tag="ACHK07_000881"
     CDS             complement(25422..26189)
                     /gene="hycG"
                     /locus_tag="ACHK07_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO8101254.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGNMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26189..26731)
                     /gene="hycF"
                     /locus_tag="ACHK07_000882"
     CDS             complement(26189..26731)
                     /gene="hycF"
                     /locus_tag="ACHK07_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO8101255.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26741..28450)
                     /gene="hycE"
                     /locus_tag="ACHK07_000883"
     CDS             complement(26741..28450)
                     /gene="hycE"
                     /locus_tag="ACHK07_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO8101256.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28468..29391)
                     /gene="hycD"
                     /locus_tag="ACHK07_000884"
     CDS             complement(28468..29391)
                     /gene="hycD"
                     /locus_tag="ACHK07_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO8101257.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29394..31220)
                     /gene="hycC"
                     /locus_tag="ACHK07_000885"
     CDS             complement(29394..31220)
                     /gene="hycC"
                     /locus_tag="ACHK07_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO8101258.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31217..31828)
                     /gene="hycB"
                     /locus_tag="ACHK07_000886"
     CDS             complement(31217..31828)
                     /gene="hycB"
                     /locus_tag="ACHK07_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO8101259.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31953..32414)
                     /gene="hycA"
                     /locus_tag="ACHK07_000887"
     CDS             complement(31953..32414)
                     /gene="hycA"
                     /locus_tag="ACHK07_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO8101260.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32626..32976
                     /gene="hypA"
                     /locus_tag="ACHK07_000888"
     CDS             32626..32976
                     /gene="hypA"
                     /locus_tag="ACHK07_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8101261.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32980..33852
                     /gene="hypB"
                     /locus_tag="ACHK07_000889"
     CDS             32980..33852
                     /gene="hypB"
                     /locus_tag="ACHK07_000889"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO8101262.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33843..34115
                     /gene="hypC"
                     /locus_tag="ACHK07_000890"
     CDS             33843..34115
                     /gene="hypC"
                     /locus_tag="ACHK07_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO8101263.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34115..35236
                     /gene="hypD"
                     /locus_tag="ACHK07_000891"
     CDS             34115..35236
                     /gene="hypD"
                     /locus_tag="ACHK07_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO8101264.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35233..36243
                     /gene="hypE"
                     /locus_tag="ACHK07_000892"
     CDS             35233..36243
                     /gene="hypE"
                     /locus_tag="ACHK07_000892"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO8101265.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLASLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36317..38395
                     /gene="flhA"
                     /locus_tag="ACHK07_000893"
     CDS             36317..38395
                     /gene="flhA"
                     /locus_tag="ACHK07_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO8101266.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38440..38868
                     /locus_tag="ACHK07_000894"
     CDS             38440..38868
                     /locus_tag="ACHK07_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO8101267.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(38907..39251)
                     /gene="ygbA"
                     /locus_tag="ACHK07_000895"
     CDS             complement(38907..39251)
                     /gene="ygbA"
                     /locus_tag="ACHK07_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO8101268.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(39328..39462)
                     /locus_tag="ACHK07_000896"
                     /pseudo
     CDS             complement(39328..39462)
                     /locus_tag="ACHK07_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39538..42099
                     /gene="mutS"
                     /locus_tag="ACHK07_000897"
     CDS             39538..42099
                     /gene="mutS"
                     /locus_tag="ACHK07_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO8101269.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EIRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42205..42861
                     /gene="pphB"
                     /locus_tag="ACHK07_000898"
     CDS             42205..42861
                     /gene="pphB"
                     /locus_tag="ACHK07_000898"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8101270.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELNDMEQKEAIGLLLKFHQLPHIIEITNDIIKYVIAHADYPGNEYLFGKQIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMLDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42902..43138)
                     /locus_tag="ACHK07_000899"
     CDS             complement(42902..43138)
                     /locus_tag="ACHK07_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO8101271.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(43149..44576)
                     /locus_tag="ACHK07_000900"
     CDS             complement(43149..44576)
                     /locus_tag="ACHK07_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO8101272.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESSYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(44576..45169)
                     /locus_tag="ACHK07_000901"
     CDS             complement(44576..45169)
                     /locus_tag="ACHK07_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO8101273.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            45316..45723
                     /locus_tag="ACHK07_000902"
     CDS             45316..45723
                     /locus_tag="ACHK07_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO8101274.1"
                     /translation="MALRNKAFHQLRQLFQQHTSRWQHELPGLTKPQYAVMRAIADKP
                     GIEQVALMEAAVSTKATLAEMLARMENRGLVRRDHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(45843..46835)
                     /gene="rpoS"
                     /locus_tag="ACHK07_000903"
     CDS             complement(45843..46835)
                     /gene="rpoS"
                     /locus_tag="ACHK07_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO8101275.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46898..47986)
                     /gene="nlpD"
                     /locus_tag="ACHK07_000904"
     CDS             complement(46898..47986)
                     /gene="nlpD"
                     /locus_tag="ACHK07_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO8101276.1"
                     /translation="MVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGT
                     TSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYS
                     GSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGN
                     AITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAP
                     VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAII
                     ATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS
                     TGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(48177..48803)
                     /gene="pcm"
                     /locus_tag="ACHK07_000905"
     CDS             complement(48177..48803)
                     /gene="pcm"
                     /locus_tag="ACHK07_000905"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO8101277.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48797..49558)
                     /gene="surE"
                     /locus_tag="ACHK07_000906"
     CDS             complement(48797..49558)
                     /gene="surE"
                     /locus_tag="ACHK07_000906"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO8101278.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPAEQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(49539..50588)
                     /gene="truD"
                     /locus_tag="ACHK07_000907"
     CDS             complement(49539..50588)
                     /gene="truD"
                     /locus_tag="ACHK07_000907"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO8101279.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(50585..51064)
                     /gene="ispF"
                     /locus_tag="ACHK07_000908"
     CDS             complement(50585..51064)
                     /gene="ispF"
                     /locus_tag="ACHK07_000908"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO8101280.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(51064..51774)
                     /gene="ispD"
                     /locus_tag="ACHK07_000909"
     CDS             complement(51064..51774)
                     /gene="ispD"
                     /locus_tag="ACHK07_000909"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO8101281.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51793..52104)
                     /gene="ftsB"
                     /locus_tag="ACHK07_000910"
     CDS             complement(51793..52104)
                     /gene="ftsB"
                     /locus_tag="ACHK07_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO8101282.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(52298..52621)
                     /gene="ygbE"
                     /locus_tag="ACHK07_000911"
     CDS             complement(52298..52621)
                     /gene="ygbE"
                     /locus_tag="ACHK07_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO8101283.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(52671..53276)
                     /gene="cysC"
                     /locus_tag="ACHK07_000912"
     CDS             complement(52671..53276)
                     /gene="cysC"
                     /locus_tag="ACHK07_000912"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO8101284.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(53276..54703)
                     /gene="cysN"
                     /locus_tag="ACHK07_000913"
     CDS             complement(53276..54703)
                     /gene="cysN"
                     /locus_tag="ACHK07_000913"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO8101285.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(54705..55613)
                     /gene="cysD"
                     /locus_tag="ACHK07_000914"
     CDS             complement(54705..55613)
                     /gene="cysD"
                     /locus_tag="ACHK07_000914"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO8101286.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55865..56902
                     /gene="iap"
                     /locus_tag="ACHK07_000915"
     CDS             55865..56902
                     /gene="iap"
                     /locus_tag="ACHK07_000915"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO8101287.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56923..57500
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56984..57012
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(57597..57890)
                     /gene="cas2e"
                     /locus_tag="ACHK07_000916"
     CDS             complement(57597..57890)
                     /gene="cas2e"
                     /locus_tag="ACHK07_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO8101288.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57887..58810)
                     /gene="cas1e"
                     /locus_tag="ACHK07_000917"
     CDS             complement(57887..58810)
                     /gene="cas1e"
                     /locus_tag="ACHK07_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO8101289.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGYIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(58807..59457)
                     /gene="cas6e"
                     /locus_tag="ACHK07_000918"
     CDS             complement(58807..59457)
                     /gene="cas6e"
                     /locus_tag="ACHK07_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO8101290.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(59439..60185)
                     /gene="cas5e"
                     /locus_tag="ACHK07_000919"
     CDS             complement(59439..60185)
                     /gene="cas5e"
                     /locus_tag="ACHK07_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO8101291.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(60196..61251)
                     /gene="cas7e"
                     /locus_tag="ACHK07_000920"
     CDS             complement(60196..61251)
                     /gene="cas7e"
                     /locus_tag="ACHK07_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO8101292.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(61266..61802)
                     /gene="casB"
                     /locus_tag="ACHK07_000921"
                     /gene_synonym="cse2"
     CDS             complement(61266..61802)
                     /gene="casB"
                     /locus_tag="ACHK07_000921"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO8101293.1"
                     /translation="MSIVKEEHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(61799..63361)
                     /gene="casA"
                     /locus_tag="ACHK07_000922"
                     /gene_synonym="cse1"
     CDS             complement(61799..63361)
                     /gene="casA"
                     /locus_tag="ACHK07_000922"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO8101294.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPDVPGAQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPQKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDPDELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(63458..>66114)
                     /gene="cas3"
                     /locus_tag="ACHK07_000923"
     CDS             complement(63458..>66114)
                     /gene="cas3"
                     /locus_tag="ACHK07_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO8101295.1"
                     /translation="TFFDFWGKTRRGEKEGGDDYHLLCWHSLDVAAMGYLMVKRNCFG
                     LADYFYQLGISDKEQAAQFFAWSLCWHDIGKFAHSFQQLYLPPELKIQEGARKNYEKI
                     SHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDN
                     FLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKQLSWYISATVVLADWTGSS
                     TRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFTGINTLFPFIENPTPLQQ
                     KVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANTM
                     YDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLIGSEEPDEQAFSQGCA
                     AWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMS
                     CILEGLIERQARGGNSVILLSATLSQQQRDKLVTAFARGTEGQQEAPLLEKDDYPWLT
                     HVTQTDVNSHRVATRKDVERSVSVGWLHSEQECIARIESSVSQGKCIAWIRNSVDDAI
                     KVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQV
                     LEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAP
                     VWDDSPGDEWFGRAMRNSAFVYPDHGRIWLTQRVLRGQGAIQMPHAARLLIESVYGED
                     VVMPEGFARSEQEQVGKYYCDRTMAKKFVLNFRPGYAASINDYLPEKLSTRLAEESVS
                     LWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQE
                     PEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT        15587 a        18183 c        17015 g        15329 t
ORIGIN      
        1 tactgcagat tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg
       61 gaggattact cgccttgcgg ctcgcccttc gggccgttgc taaagcaacg ttatcctccc
      121 tggtgcctgc ggttagtacc gccacctctt ccataccttc atcgctgaag gttacggaga
      181 atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
      241 tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct
      301 tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac
      361 gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat
      421 attactttct gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact
      481 tcacgttcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaatggag
      541 gtctgacaga tagcagtaat gtaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa
      601 gcgaaaagac tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag
      661 acgcggaaag attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc
      721 tcttcacggt gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc
      781 tttaccccta aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga
      841 cgagtcagaa tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat
      901 cccggcatta tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatctaa
      961 gcagtcatgg cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct
     1021 gactttatcg ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc
     1081 ttacgcttat attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca
     1141 ggtaaggccg cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg
     1201 ccacccacct gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg
     1261 tccttagata cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata
     1321 attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca
     1381 acaccgctaa gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa
     1441 agatttgcgc tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac
     1501 tgacgcgtac gttccagtac tgagcgcact ttatcagcca gattattgct atcgcctttc
     1561 agcagatgtg cgacttcgct taagcgatcg ctgtctgcat gaacggtggc gatagcgcct
     1621 tctccggtta ccgcttcaat acgacgaacg cctgcagcag tacccgattc agagatgatg
     1681 cggaacagac caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag
     1741 aaatcgccca tgctcagcac gcgtacgcgc tcatcatact tctcgccgaa cagcgccatc
     1801 gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caaattgcga
     1861 cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct cttctggttt catcgcttcg
     1921 ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca
     1981 tgagtaccca gaacctggcg cagcgcagca tgcatcaggt gcgttgcgga gtgattcaga
     2041 cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga
     2101 gaacccgcag caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc
     2161 accacgaagg agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat
     2221 tccgcataga atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca
     2281 tcaaccgctt taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca
     2341 tagcctttaa attcagatgc actgtcaaca cggatcattg cgttgtaatc ggcaccaaag
     2401 ccgctggctt cgcgcgcgcg acgacgctgc tcttccattg cagcttcaaa accagcttcg
     2461 tcaactttga tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag
     2521 gtgtcgtaca gacggaaagc agtttcacca tccagcgtat caccagaaag ttttgccagc
     2581 tcttcatcca gcaacgccag accgcgctcc agagtacgag caaactgctc ttcttcagtc
     2641 ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata
     2701 acgtcgatca gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatatta
     2761 ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac
     2821 ggcattacgc catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc
     2881 agcgatttat tgctcagatc ggttgcgcca gtgactttcg ctaccgcctg gatcaacgtg
     2941 cggaacaggt cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc
     3001 agacccatac cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atcggcctgg
     3061 cggttgaact gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc
     3121 ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac
     3181 gggccgcacg gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct
     3241 ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt
     3301 tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca
     3361 aaccattttt cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag
     3421 tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg
     3481 acgttttcca ggtcgttgtg tttaccaccc gcacgcacac agcgttggga agtggtagcg
     3541 cgggaataat tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg
     3601 ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga
     3661 tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc
     3721 ataattatcc tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg
     3781 ccgggctggt aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac
     3841 cgtatgcgtt cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc
     3901 acgatagagc agaaaacgct ggatcttaac tttttctgaa aagacagttg acaaaggttc
     3961 accatatttt cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg
     4021 catcgctttt tctgtcgctt cgcgggaaat acctttctga ttcagttcct ggcgaatacg
     4081 cgcaggtcca taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc
     4141 atcgagatag ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt cttccgtggc
     4201 atcaatctct tccgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc
     4261 ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata
     4321 tgccgggcga cgggacgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa
     4381 gggccgcaga tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtgtgca
     4441 gaatgttccc gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag
     4501 tgctggcctg ttcccctcac cctctcccca aaggggcgag gggaccgtcc gtgcacacat
     4561 tatctattat catcaataac gaacgattaa aaatcttcgt tagtttctgc tacgccttcg
     4621 ctatcatcta cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact
     4681 ttcttctcga tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct
     4741 ttaccctgac cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc
     4801 ttctctttta cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg
     4861 atctggaatt cagcctgttt aaacggcgca gcgattttgt tcttcaccac tttcacgcgg
     4921 gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga
     4981 cgaacagagg cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac
     5041 atcacaccaa ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc
     5101 aggttacccg ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga
     5161 gagtcgccga tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg
     5221 acgataacgt ctactgcgcc agaacgcgcc agggcgtcac agatttccag tgcctgctcg
     5281 ccggtgtccg gctgggagca cagcaggttg tcgatatcga cgcccagttt acgtgcgtag
     5341 attgggtcca gcgcgtgttc agcatcgata aacgcacagg ttttaccttc acgctgcgct
     5401 gcggcgatca cctgaagcgt cagcgtggtt ttaccggaag attccggtcc gtagatttcg
     5461 acgatacggc ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg
     5521 gtagagatgg tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta
     5581 ccaaattgtt tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg
     5641 atagccattt ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa
     5701 gcaattatac tgtatgctca tacagtatca agtgttttgt agaaattgtt gccacaaggt
     5761 ctgcaatgca tacgcagtag cctgacgacg caccgcatca cggtcgccgc tgaagcattc
     5821 ccgccgggta atgccttcac cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg
     5881 cttctcttca ctgccgccat ccggcccggc gataccacta atagacacgg cgtaatcagc
     5941 acgagccgct ttcagtgcgc ctatcgccat ttccaccacg acgggttcgc tcaccgcgcc
     6001 atgctgcgcc agcgtctctt cacgtacgcc gatcatctgc gctttggctt cgttactgta
     6061 ggtgacaaat ccgcgttcaa accaggcgga gctaccggca atatcggtaa tcactttcgc
     6121 tacccaaccg ccggtacaag actcagcagt tgttacggtt gcgccacggg ctttcagcgc
     6181 ctgcccaacc tgttcgctta actgcatcag ttcactgtca gtcatcacaa cctctgtcag
     6241 tcaaaaattt atgccggaca agatagcact ttcgtcagga agatggtgtt ggcgttgcaa
     6301 ttttacgagg aggattcaga aaaaagctga ttagccagag ggaagctcac gctcccctct
     6361 tgcaaatagt tactgcacgc gcgccagcgc cacggcttgt cctaactgcc agaccgccat
     6421 tgcgtaatgg gtgctgtggt tataacgggt aatggtgtag aagttcggca gaccgtacca
     6481 gtactggtaa ccggtaccga catccagacg cagcaggctg gcttgttgat ggttgcccag
     6541 cggctgctgt ggcgttaaac ctgccgcggc aagctgcgaa atgctgtact tggttttgaa
     6601 gccatttggc aagcctggag cctgaccgtt tgccattacc gcgacctgat cgcctttcac
     6661 ccaaccgtgc gctttgaaat aattcgccac gctaccgatc gcatcaaccg gatcccacag
     6721 gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg acggcataaa
     6781 ctgtccgtag cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc
     6841 gtcgcgcgcc atcagcagga aggtttccag ctcgccagag aaatactccg cgcggcgtgg
     6901 gtagttaaat gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc
     6961 ccagcgggtt tcaacgccga taatcccgac gataatttcc ggcggtacgc catacacctg
     7021 ccacgcgcga ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt
     7081 gtccggcgta ataaattttt tgcgataacg cagccatgcg ccgttcggac ctgatggcgg
     7141 tttcaccgat gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgttt
     7201 cgcctgggag agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccatttt
     7261 gtcgatgaac tgctgggcat tcgggttatt ggcgaagtca ccgcccatct gcatcacatt
     7321 gtgctgcggc tcaagcagga agccgccaga cggcgttccg gtggtcgttt cagtctcagt
     7381 aggttttggc ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata
     7441 acgacgcttg aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag
     7501 catcacgccc cgcacaagga agcggtagtc actgcccgat acggacttta cataactcaa
     7561 ctcatccccc tcgctatcct tttattcaaa ctttcaaatt aaaatattta tctttcattt
     7621 tgcgatcaaa ataacacttt taaatctttc aatctgatta gattaggttg ccgtttggta
     7681 ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag
     7741 tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt
     7801 cttccgctgt tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag
     7861 catcgaattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg
     7921 ctaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccctaagtct gggtcgcttt
     7981 atgccggaaa agtacaaacc cagctactac gcggccgcct cttatagctg ccactcaatg
     8041 aatggcctct tcccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc
     8101 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagctatct gctggttggt
     8161 ctggtcacca acttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa
     8221 aagatgggta ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta
     8281 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
     8341 gcaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggtac
     8401 agcttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
     8461 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatttat ccgaaacggc
     8521 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccactggcg cagtacattg
     8581 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
     8641 caccacaacc ctcttccgtg ggccgtgact atgacaccag caagaaaatc accgaacaaa
     8701 gcgatggttt actggcgaag gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc
     8761 aatctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg
     8821 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
     8881 ttgctccgct ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc
     8941 cgctgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
     9001 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttacct gcgctgtttg
     9061 ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
     9121 gtcaggacac ggttcgcgtc ggtgtccctt ctgttctggt gagccgcttt ttaaccggcg
     9181 caccgactgt actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac
     9241 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
     9301 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgcctg cggatctcga agagtattgc
     9361 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcgctc
     9421 gggcagcatc gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa
     9481 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
     9541 catgtggcag gccctgtccc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
     9601 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
     9661 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
     9721 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg
     9781 tacggttttg gtgctgacgc caccagtgag caaagcgttc tggcgctctc tcgtggggta
     9841 gatgaaatct ttggtcgcgt ggatttgctg gtctacagcg ccggaattgc aaaagcagcc
     9901 tttatcagcg acttccagct cggtgatttt gaccgttcgc tacaggtgaa tctggtgggt
     9961 tatttcctgt gtgcgcgtga attttcgcgt ttgatgatcc gcgatgggat tcagggacgc
    10021 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    10081 gcagcgaaat ttggtggcgt agggctgact caatcactgg cgctggatct ggcggagtac
    10141 ggcattacgg tgcattcact gatgctcggt aacctgctga aatcgccgat gttccagtcg
    10201 ctgttgccac aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    10261 atcgataaag taccgctcaa acgcggctgc gattatcagg atgtgctgaa tatgctgctg
    10321 ttctacgcca gtcctaaggc atcgtactgc actggtcagt cgatcaatgt caccggcggt
    10381 caggtgatgt tctgatcaac agcggagatc cgttaaggat ctccgtggga ctaatagaat
    10441 gcctgatgcg ctacgctcat caggcataca ggacttccgc cactacatta aggaaaagtt
    10501 atggtatccg cactcatcac tgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc
    10561 ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggcgggtt
    10621 ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat cgccctggac
    10681 gatcagcagc gcatcgccga caccttgttt atgaaaggac tgaccgtctt cgcccgaccg
    10741 caaaaaattc ccgcaattac cggcatgcat gcgggtgatt tacagcccga tgtgatcttt
    10801 ccccatgatc cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa
    10861 tttcgttgtg aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc
    10921 acgcccatga aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt
    10981 aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa
    11041 gatctggtca ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtggtgttg
    11101 aataaagagg aatccgatcc gcctatcgat cataaaacac tcatcaacac ccacaagaaa
    11161 gagctgatag cagaagctgc cgttggtttt attcatgatg gcgattcgat cattcttgat
    11221 gctggcagca ccgttttgca gatggtgcct ctgctctcgc gctttaataa catcacggtg
    11281 atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc
    11341 ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat ttcacgggca gctggcagag
    11401 aatgccttcg agcatttcac cttcgacaaa ttattcatgg gtactgacgg catcgatctc
    11461 aatgcgggcg taaccacctt caacgaggtc tataccgtca gtaaggcaat gtgcaatgcc
    11521 gcgcgcgaag tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta
    11581 gtttgcagtc ttgaaagcgt cgataagctg attaccgacg cagatatcga tccggcgttt
    11641 cgtcaggcgc tggaagagaa agggatcgat gtgatcataa ccggagagag caatgagtga
    11701 agcactactg aacgcgggac gtcagacgtt aatgctggaa ttgcaggaag caagccgttt
    11761 accggaacgt ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg
    11821 caaagtggtg gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac
    11881 gcttgccagt accggcacac cggctttttt tgtccatccg gcagaagcgc tgcacggcga
    11941 tctggggatg atcgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggggcgaa
    12001 ggaactggat ctgattattc cgcgtctgga agacaaatct atcgcgctgc tggcgatgac
    12061 cggcaaaccg acgtcaccgc tgggcctggc ggcgaaagcg gtgctggata tctccgtaga
    12121 acgcgaagcc tgcccgatgc accttgcgcc gacctccagc accgtcaata ccctgatgat
    12181 gggtgacgcg ctggcgatgg cagtcatgca ggcgcgcgga tttaatgaag aagattttgc
    12241 ccgctcccac cctgctggag cactgggcgc tcgcttgctg aataaagtgc atcatctgat
    12301 gcgccgtgac gatgccatcc cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct
    12361 ggaactcagc cgcaccggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca
    12421 aggcgtcttt accgacggcg atttacgtcg ctggctggtt ggcggcggcg cactcaccac
    12481 gccagtcaat gaagcgatga cgacaggcgg caccacatta caggcacaaa gtcgcgccat
    12541 cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tggatgaaaa
    12601 cggcaaactc gtcggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc
    12661 cttcaatccc agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcattcg
    12721 cgcgcaggct gcccagttgt gatgattttg tgccagtgcc tgacgaatag tttcacgctg
    12781 gaacgcttct gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg
    12841 cggcggtaac gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc
    12901 gttgcgggtg gctctcgcca gcactaccgc ccgatgaata gcatgttcca gttcgcgcac
    12961 gttccccgga aaacgatagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg
    13021 ggagagcccc tgccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc
    13081 atcgccccgc tcacgcagcg gcggcaccga aagtggaaac acgctcaggc ggtggaacaa
    13141 gtcagcgcga aatcgccctg ccagcacctc ttcgcgtaaa tcgcggttag tcgccgccag
    13201 cacgcgcaca tcgacccgca aactacggtc atcgccaacg cgctgaatat cgccatactg
    13261 caacaccctc agcagcttgg cctgcaatgc caacgacaac tcgccgatct catccagaaa
    13321 cagcgtgccg ttatccgcca tttcaaactt cccactgcgg ttactgattg cgccagtaaa
    13381 cgctcctttc acatgcccga acaactcact ttccgccaca ctttccggca gtgcggcaca
    13441 gttgagatag accagcggat tcaccgcccg tggcgaggcc tcatgaatcg ctttcgccac
    13501 cagctcctta ccggttcccg tctcaccgct gatcaggacg ttgagatcgg acgccgccac
    13561 aatctcaatc tcttttttca gttgcgtcat gcctggcgac agaccgatca tctgcgtctg
    13621 tttcaccgct tcaaatcgag cggcatcgcc cggcagcata ttctggcttt ccagttgctc
    13681 aatcagcaac gcattgctta acgctcccgc cgccagcgcg gcaatcaggc gtaactcttc
    13741 gtcgctgaaa acatcgaact gatcgggctg catcccgtcg agcgtcaatg cgccgatcag
    13801 gttttgcccg gcaaacagcg gcagaccaac gcaggcgtga accttcagac tctcctgccc
    13861 tggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc
    13921 acccgcgcgg gcaatcgctt ccagccgtgg atgaccttcc agcgcaaagc gtctaccgag
    13981 tacatccttc gccagcccgt cgatggcaag cggaataaac tgccgcgagt cgtaacgtag
    14041 caacgcagac gcatcgcact ccagcacctg acgcagcgtg gtgatcaggc gctgaaaacg
    14101 atcctgatga ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga
    14161 aaaactcatc tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga
    14221 ttgatagtca ttttgactac tcactaatga gcataatttt atttatagag taaaaacaat
    14281 tagataaaaa actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa
    14341 taaattgagg ttgctatgtc tattgtggtg aaaaataaca ttcattgggt gggtcaacgt
    14401 gactgggaag tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagctac
    14461 aacagctacc tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc
    14521 agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc
    14581 gtgattaacc atgcagaaga ggaccacgcc ggggcgctga ccgaactgat ggcacaaatt
    14641 cccgatacgc cgatctactg tacagccaac gctatcgact cgataaatgg tcatcaccat
    14701 catccggagt ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc
    14761 aaacagctca tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac
    14821 ctgacaggcg acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag
    14881 catctgttca acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac
    14941 gccaatatcc tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc
    15001 tttaacttac cagtcgatat gatagcaacc tcccacggcg tggtctggcg cgataacccg
    15061 acgcaaattg tcgagctgta cctgaagtgg gccgctgatt atcaggaaga cagaatcacc
    15121 attttctacg acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg
    15181 attgcggaaa ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa
    15241 aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg
    15301 aataacgtga tgatgccgaa aatcgccggg ctggtggagg agatgaccgg attacgcttc
    15361 cgtaacaaac gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt
    15421 ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agcgaaatgg
    15481 cgaccagacc aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag
    15541 tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tagttaaaga agaaacctct
    15601 gccaccacga cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac
    15661 gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc
    15721 ccggataact tcctctgccc ggaatgctcc ctcggcaaag acgtctttga cgaactggca
    15781 tcggaggcaa aatgagcaac ggcattgtga tcattggttc gggctttgcc gcccgccaac
    15841 tggtgaaaaa tattcgcaaa caggacgcca ctattccatt aaccctgatt gccgccgaca
    15901 gcatggatga gtacaacaaa ccggatctca gccatgttat cagccagggg caacgtgccg
    15961 atgaccttac ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cgcctgtttc
    16021 cgcacacctg ggtgatgaat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc
    16081 agtggcaata cgacaagctg gtgctggcaa ctggtgccag cgcctttgtc ccgcctgtgc
    16141 ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc
    16201 aactgcggga tgcccgacgc gtgttgattg ttggcggtgg tttgattggt agcgaactgg
    16261 cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc
    16321 tggcgtcgtt aatgccacca gaagtaagca gccgcttgca gcatcggttg acggagatgg
    16381 gcgttcatct gctgttgaaa tctcagttgc aggggctgga aaaaacggat tctggcattc
    16441 tggcaacgct ggaccgccag cgtagcatcg aagtggatgc ggtaattgcc gccaccggac
    16501 tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg
    16561 tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa
    16621 ttaacggtca ggtactgccg ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa
    16681 aaaatcttct cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa
    16741 cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaga
    16801 ttaataccga acgccaggga atggtggcgc gcggtgttga cgatgcagac caacttcgcg
    16861 cctttgtggt cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccgg
    16921 tgtaggcggg ctactgtgcc caaaatgtcg gatgcgacgc tgacgcgcct tatccgacct
    16981 acggggagcg catgtgttgg tcacataatg cgttcatgtc gcatccgcct actgtgccca
    17041 aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg
    17101 gataaggcgt ttacgccgca tccggcaatg gtgtccaaat gcaacacgat ttatccgttc
    17161 tggacttcac ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg
    17221 ccatcacccg ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc
    17281 agacgtgcac gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata
    17341 ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga
    17401 aacgcccacg cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg
    17461 gtgagatcca gttgattgtc caccagcggc attgtcaccg gatgcgtcac tccgtggcat
    17521 gaggctgcga gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc
    17581 gcacagccca gtgccgccgc cactgcatcg aacacccgcc cacacgatga cgccagcggc
    17641 gcgttaattc cacgctcaat ggcccgcgcc agaacgctcc agttttgctg ttgtacactt
    17701 gccgtttcag ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggtta
    17761 cgccacggct gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc
    17821 aggtgctcac attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccgttc
    17881 tcccccatac cgataccgtc gagcgtcaaa gcaatgacat caccgccctc cagtggccag
    17941 tgatgttctg ccagacacgc cgccgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc
    18001 agattcattt cgcgcgccca ctggctggag acatagcccg gatgtgcgtc atgcacaacg
    18061 tattgcgggg tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc
    18121 tggatgccat cgtcacttaa atcgcccaga tgctggctca acaccgcttg ttcaccgcgc
    18181 accaggcaga aggtattttt cagatccgcg ccgagacaca gcacaggcgg aacattttta
    18241 aagcccggag gcaaagccag cgcatccggc acataccccc gcgaacggcg tagcatttcg
    18301 ccgctttcgc gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag
    18361 aatccgtcgg caatgccctg caaatctgcc agcgcctgtt cgttgctgat agccggtggt
    18421 ttaccgctca ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa
    18481 tgctggagcg ggttcgcagg caacattacc ccgacttcgt taaggtcagg ggcgatatca
    18541 tcacaaagct caggtacgta ttttttatcc accagcacaa tcggcgcggc gggcgtggta
    18601 agcaactggc gcgcagcgtc tggtaaaccg tcagccactg gcaacatgac cgccagcggt
    18661 ttcgccgggc gatgtttgcg cgcccgcagt gtcgccaccg cgttactgtt acgtgcatcg
    18721 caggcaagat gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt
    18781 gcgatagccg cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca
    18841 agatgcgggc cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg
    18901 tcgcggtact ctttatcaca ggctggacac agcggaaacg ccgccatcac ggtaaacggg
    18961 cgatcgtaag gcatggcgcg aataatggtg aaacgcggac cgcagtgggt acagttgata
    19021 aacggataac gccagcgtcg ctcgccaggg gtattcattt cggcaaggca ggcagggcaa
    19081 gtggcggcat cgggaacaat ttgcgtattc atggcgccgc ccgcgctctg gcggatagtg
    19141 aactcggtgg gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc
    19201 agcggcgggc agtgctgatg caattgaaca agaaacgttt ccgggtcttc cagcagccgg
    19261 acttctacgc catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagc
    19321 tgccagacaa acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca
    19381 ccgcaagatg tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgtc
    19441 cagccggaca cgacagcgtc gcatccggta gtcacaggtc ggcgatactg cccgcttcgt
    19501 attctacgaa tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga
    19561 acatcagcgc cgtgcggcga cgtttttctg cgctcagttg ttcaagttta ttacgatcga
    19621 cacaaatcag cgcatgagtc gggcaagccg ccatacacgc cggaccgtct tcacgatggt
    19681 tgcacaggtc gcatttattg gcttcggctt tgtcagcccg tacattcagc cctgcgccgc
    19741 tgttgcgaat caccggacgt accaccactt ccatcgcgcc atacgggcaa gccacaacgc
    19801 aggttttgca accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacggctga
    19861 tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg
    19921 ccgtggaaat gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca
    19981 gcgatgcaca gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc
    20041 aaccaataca tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaatg
    20101 acagttaatg cgccgataca ttcacaaatc atgccagttt ttaattcact gttatttaag
    20161 gaaattaatt tctgtaatgc aggaaaaaaa gatgtcatcg acactagtga cgatgacatg
    20221 tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggcggatca gttttccgct
    20281 gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat
    20341 ttcctgaatc atctctgtta ccggaatttt tacgctggag agcgccggaa cggtgtaggg
    20401 ggcaatagcg atatcatcga atcccataac tgacacctgc tctggcaccg ctacgccgcg
    20461 ctcgtgtaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct
    20521 aaatttagcc ccacgttcga gcaacatttc taccccttcg gccccgctgg caggcgtcca
    20581 tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt
    20641 atacccggca agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat
    20701 ctcctgatgt ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc
    20761 gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttgagcacca ttatcggctg
    20821 actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg ggtaaatcat
    20881 aatcgcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct
    20941 gtgtttacca tctgccaata gtaactgtcg ccctttctct tccgccattc gcgcggcatg
    21001 aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta ccagccccag
    21061 cgtctgagta ctcttcgccg acagattacg cgccagcaag tttggacggt aaccgctctc
    21121 ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag
    21181 cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt
    21241 catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc
    21301 cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata aaaaatgaac
    21361 aattaactct cttgcttatt tggtgacaac tattcatgat tttgtgaaac cggtttctta
    21421 attccgtttc agcatcggca tttttccgtc acgtcgactg ataacaacca catctaccct
    21481 actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac gctcggtgat
    21541 agcggcactg ggcggcgttg ataacatctc ggcagtcacg cactgtatga cgcgtttgcg
    21601 ctttgttatc aaagatgatg cgcttatcga cagcccgacg ttaaaaacca tccccggcgt
    21661 gctcggcgtg gtacgcagtg acaaccagtg tcaggtgatt atcggcaata ccgtttcaca
    21721 agcctttcag gaaatcgtca gcctgctgcc gggagatatg cagcccgcac agcccgtagg
    21781 taaacccaaa ctcacgctac gtcgcattgg tgcggggatc ctcgatgcgc tgatcggcac
    21841 catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc tggcgatgat
    21901 cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc tgaatgtgat
    21961 tggtgacggt gctttcttct tcctgccgct aatggtcgcg gcatctgccg ccatcaaatt
    22021 taaaaccaat atgtcgctgg cgattgccat ctcgggtgtg ctggtacatc cgagttttat
    22081 tgaactgatg gcgaaagcgg cccaggggga acatgtcgaa tttgccctga tcccggtcac
    22141 cgcggtgaaa tacacctaca cggtgatccc ggcgctggtg atgacctggt gcctgtcata
    22201 tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc tcaagccaat
    22261 gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga ttggtatctg
    22321 gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg gctggctttc
    22381 agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc accgcgtctt
    22441 tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg tcatgccgtc
    22501 agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt ggaaaacgaa
    22561 aaacccggaa ctgcgccaga ctgcgctggc tgcggcggca tcagccatta tggcggggat
    22621 ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta tcgccagtct
    22681 tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca gccactcaat
    22741 ggcagcgccg gggctattta ccagcgtgca gttcttcgat ccggcgaatc caatgagcat
    22801 cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc tcacactgtt
    22861 gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa agcatcagag
    22921 cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat gtcagtattt
    22981 ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg tgcgttccgt
    23041 gaaggtggca aaggactgac aacggtcgat atgatcccac acggcgagca tcgaatggcg
    23101 gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta tcccagccat
    23161 gaggcgacgg gtttttatca tcgttataaa gaagatatcg ccctgatggc agagatggga
    23221 ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg cgatgaaatc
    23281 acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg taaaaagtac
    23341 ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct ggtcaccgaa
    23401 tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc ccggacctgc
    23461 tttgaagcat ttgatggtct ggtgaaatac tggctaacct tcaatgaaat caacattatg
    23521 ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa tcaggatcag
    23581 gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac caaaatcgcc
    23641 catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggcaa cttctaccct
    23701 tacagttgca agccggaaga tgtctgggcg gcgctggaga aagatcggga aaacctgttt
    23761 tttatcgatg tgcaggcgcg gggcacgtat ccggcttact ctgcccgcgt attccgcgaa
    23821 aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa caccgtcgat
    23881 tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa cgccaacaac
    23941 agcagtgcgg cgaacgtggt gaaatcgctg cgtaacccgt atctgcaagt gagcgactgg
    24001 ggctggggaa ttgatccact cggtctgcgt atcaccatga acatgatgta cgaccgctat
    24061 cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact tgctgccaat
    24121 ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg cgcaatgggc
    24181 gaagcgattg cagacggcat tccgctgatg ggctacacca cctggggctg tattgattta
    24241 gtttccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctttgt tgaccgtgac
    24301 gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg gtataaaaaa
    24361 gtgattgcca gtaatggtga agatttagag taagtaaccg tgccggatgc ggcgtgaacg
    24421 ccttatccgg cctacatagt tcgtgcgaca tgtaggcccg ataagcgcag cgcatcgggc
    24481 aatttagcat ttggcatcag agctggttca ccccatcaag aacatccctg tcctgattcc
    24541 ttattgaaaa actactcttc ttccaccgct aactgcgcga agccgccgtt cccttcccag
    24601 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgggt catcgggtag
    24661 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat atcttctttc
    24721 aactgatcga taaggtagtt caacggcatg ttatgggtag tcatcataaa catctcggcg
    24781 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    24841 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgcg
    24901 ctaccgccgt caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    24961 agcggacctg cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    25021 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    25081 taacagcatt gtactccatt cctgctgctg cgaagtttgc gctgcccggg ctttatcgaa
    25141 tgcattggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    25201 accttccatt ttgcggcggg cttcgctgcc cgcctccaga gtggcaatcc atgccagata
    25261 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    25321 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    25381 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    25441 tctacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    25501 agccagcgtg caacctgttc ttcgccctgc cctaactgtg taaggtaatc atcggcaatc
    25561 tgacgaccgt aacgataacc cgccagtcga cgtgcttcgc gatccacttt cacacgcagc
    25621 ggctgcacca tattgccatg cagaatttct gccggttgtt catcctgctc acccggccca
    25681 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    25741 gcaggcgttg gcgggcagcc agggatataa acatccaccg ggacgatttt atccgtaccg
    25801 ccccacacgc agtagagatc gtggaagatc ccgccgctgt taccgcaggc accgtaggag
    25861 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    25921 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    25981 atgccgaagc gttctgcatc gaacagcggc gacaaagtgg cgaaaatttc gatttcgcag
    26041 ccgttgcagc cgccgcagtc cacgcggtaa acataggcag aacgtttgat ttttttcagt
    26101 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    26161 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    26221 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    26281 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    26341 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg acagttgcac agcgcgaagc
    26401 gggactgttg caggaagtct tctttcttcc acaccgccag ttcgtactct tgcgacagct
    26461 taatcgccgc cgtcgggcag acttcttcgc agcgtccgca gaagatgcaa cgcccgagat
    26521 taaactccca ggccagctct cctgtggcga ggtcagtttc aaccgttaag gcgtttgacg
    26581 ggcaggcatt gacgcaggcc gcgcagccga tgcactgctg cgggttctgc tctggcttac
    26641 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    26701 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    26761 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc ttcttcttac
    26821 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtcgagg ctaccgataa
    26881 tcagcggcgc atcggaaacg gtattgccac gcagcatgta gcgcagggtc ggccagttcg
    26941 cgtaagtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    27001 agtggatatc atcgccgcgc ggcgcttcgg caaagcccag cgcaaagcgg tgcggaatgt
    27061 aggtaaagcc ttccaccatc agtgggccac ccggcaggtt atccagaccg tagtcgatca
    27121 tgttcagcgc ggtatagact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    27181 cctgctcgct gtggacttcc attggcagca ggccataacc aacaaacggg tgatcggcgc
    27241 gagtatcgcg agcgtgaccg ctggcacgga ccatcgggcc gacgttactg aagtcgcgag
    27301 cgatttccgg gtccagacga ccaatgccga cagtgcgttg ttccatgttc ggtgtgctca
    27361 acagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    27421 ggatcatgtc atctttcagc agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    27481 ttttacgcgc accggtaagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    27541 actgcataaa gccggagtca aagccggtaa agtggcaggc aagaccgagg ttgagcagat
    27601 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    27661 cctgaatacc catcgcgttt tccaccgacg tggtgtaggc ggtgctgtga gcaaagccgc
    27721 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    27781 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    27841 cgccatcgac gaacagacgg aagtgacccg gttcgtcaga agtgacgtgc agcggaccaa
    27901 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    27961 cggtggtcgg tgccggacgt tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    28021 catccggcca gtcatccggc agcaccagac ggcgttcatc cggcagccct accggaatca
    28081 aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc cgccggaacg cgcggcgtca
    28141 cggacggata ctccggtttg ttggcgtcaa cttcgacgcg aaccgtaatc caacacttag
    28201 tgcccttctc catcgacagc acgtagtaaa cggcgtaatg accattcagt ttgcgttcgt
    28261 cgttaccaaa cagcaccgac agccagccac cctgtttgta gtagagaaac tccaccactt
    28321 ccggcagata gttcaccttc acggtcacgg tcagctgatc tttggtctgc caggcgtggt
    28381 ccagcacgac gcccggaaat gcctcattca gcgcggcgag ataatgttga cctaattttt
    28441 cttcagacat gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa
    28501 tgcaaagcca aacccagccc aggtaatgcg cggagtaata tcaagacgca gacgggccat
    28561 gctgttttcg aacagcgcga taaccaggac gccgaccacc agtttgacga tggcaatcac
    28621 cagcgccagc agcagtccac cagcggtgaa ggtttccatt tgtccccacg gaataaacac
    28681 cccgacgaac atctgcagca ccaccagctg tttcaggctg ataccccatt tcatgacgcc
    28741 aaagccgctg ccgctatact cagagagcgg accttcctgc aactcctgct cggcttccgc
    28801 caggtcgaac ggcagtttgc ccatttcgat aaaagtggcg aacgcacagg cacaaagcgc
    28861 cagcaccagc gggatgctct ggctcagcgg ccagtgataa acggtgtcgg tgatgttgct
    28921 gatgttggtg gaaccggcaa cctgtgcggc aacccacaga ccaagcagca acatcggttc
    28981 gaccagcacg ccaagcatcg cttcacggct cgcgccgata gcggtaaacg ggctaccggt
    29041 atccagacca gaaatggcaa agaagaaacg cgcgatggca aagagataca gtaaggtgat
    29101 caaatcaccc agttgcggca gcggagaacc aacggtcacc accggcagcg cagtagcgat
    29161 agtcagcatg acgcccacca tcacatacgg cgtcaggcgg aacacccagc cggaggcatc
    29221 cgggccaacg ctctggcgac ccagcagttt gataatgtcg cgatactcct gcaacacgcc
    29281 cggcccgcga cggttatgca agcgggcgcg cgccacgcgg gttataccgg agagcagcgg
    29341 cgcaacggca aatagcacca gcgcctgaat taacggatat aaaacactca ttctcaggct
    29401 cctcgtgaaa caataatcac caccagcacc gccagttcaa ccagcgccat ccggcggaac
    29461 agcaacgcac tcccctcgca ctgccagccc ggcaccagag acaccggatt cagccatttg
    29521 cgtagtttca gcaccggcgc aaacgcctgt ttcaccggca tggcaaaacc gtgagcggta
    29581 atcaccattg atttttcgtg gtcgtagccg cacacccagg ccgcgccgcg ggaacgcgat
    29641 ggcaaacgat cgcctttgca aatcgccata atgatgaatg gcagcagcgg gcaggcaatc
    29701 agcagcaacg tgatcattgg ttgagaaacg gtggtgttag caggctccag aggcagaggt
    29761 actgcagcag agagcatcgg cagcagccac ggcgcagcaa caccgccaat tacgcagcaa
    29821 atcgccagtg ccactacgct tacgctcatc aggagcggtg cacaggtggc gttttcggct
    29881 tctttggtac gcggcgcgcc gaggaacgtg acgccataga ctttcgccat acacatcacc
    29941 gccagcgcac cggtaattgc cagccccaca gcgagcagcg gccccagcag acgggcaaca
    30001 aacgcgccac tattgctcag tttgaaaaag gattgataga taacccattc cccggcaaac
    30061 ccgttcagcg gcggcagcgc agccattgcc atcaacccga ctaacatggc gatggagata
    30121 accggcattt tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac
    30181 cagacgctcc ccgccccgag gaacagtacg cttttgaaca ggctatggtt aagcagatgg
    30241 tacagaccac cgaccagacc aagggcaatc agcgccggtt gttcgagcgc gatacccgtt
    30301 acgccagcgc ccagccccag caggatgatg ccgatatttt ccagggtgtg gtaagccagc
    30361 aggcgctgga tgttgtgctc catcagcgca tacagaccac cgacaaacgc ggtgatcatg
    30421 ccgagcacca gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg
    30481 gttaaaatgc ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc
    30541 gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccggccagc
    30601 aggccaaagc caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata
    30661 cgcatatcca gcaggcgcag atccagcgtg ccataacgct gccacagcag ccagcaagca
    30721 atcaccagca gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg
    30781 ttgctggtga ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg
    30841 aggttgctgg caatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg
    30901 ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt agaggctgac aaacagaccg
    30961 cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag agatttgcac atcgtagctt
    31021 accagcgaca gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct
    31081 gccgtataca gactaccaac cgcgccgcca attccggcta tccagccgct tagcgccttt
    31141 tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa accacgccac gccgctattg
    31201 atcagggaaa ttgcgctcat ttagcctctc cactttgagc ctgctgaaac aaggtgagat
    31261 cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg
    31321 tgttatccac cagatgcagg gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt
    31381 catcaaagct acaaaggtca catttgacgg cgatcgcgcg aatacctggc acccagtcaa
    31441 gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt
    31501 ttgccggaat atccagcgga cggctgccgg aaaattcaat tgcgccaaac gggcaggcga
    31561 tgccgcacag tttgcagctt acgcacaggc tttcattcaa ctgcacggcc ccatcaacgc
    31621 gggtgatggc gttaaccggg cagaccaccg cgcagggtgc atcttcacag tggtgacaga
    31681 gctgcggcgc agattctttt tcattcagca tcactctcag gcgcggcatt gattgcaggc
    31741 cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt gtggcagccg atacagagcg
    31801 tggagtcagc aattacaaaa cgattcacca ggcattcctc aggtgattgt catttttgac
    31861 gaaaacatgc cgatgaaatg tcattttcga cactcatcga cacgcccatc cccaaacagg
    31921 cgtaacgcct gcaaaacggg caaagcctca gctcatgctg ccgggctttg tccctttacc
    31981 agttggctta aattcaccgg cacattgttt tcaatggcgg tgtataagct gtcgtaaacg
    32041 ccagcgattt tttcgagata gtgttccaga acctgttcgc gatcgcggat gttgaccttg
    32101 ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca
    32161 tcttttgttt cgacgtaata ctcgatggtg tggctgttaa agccatccgc cagggtgacg
    32221 taaatacgaa aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc gcagctcatg
    32281 gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt gcagtagatg
    32341 tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt
    32401 tcccaaatag tcattgtcag gttacccgtt taacagagat gccagcttta agcatttttt
    32461 gtgccaactt ttaatttatt gttattaaag cgatttttaa gattttggtg aatttcgtcg
    32521 ccgtgtcgac gtgtcatttc gacatcatcg acattattca ctgcagggat aatcaacact
    32581 ggcacaatta ttgcttgtag ctggcaatag ttaatgggag gcgatatgca cgaaataacc
    32641 ctctgccaac gggcactgga attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc
    32701 gtaactgggg tctggctcaa aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc
    32761 ttttgttttg atctggtttg ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa
    32821 gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct actgacccag
    32881 cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac agattgtggc agacgacggt
    32941 ttacagattc ggcggataga aatagaccag gagtgagcga tgtgtacaac atgcggttgc
    33001 ggtgaaggca acctgtatat cgagggtgat gaacataacc ctcattccgc gtttcgtagc
    33061 gcgccatttg ccccggcggc acgcccgaag atgaaaatca ccggcattaa aacgcctgaa
    33121 tttaccccca gccagactga agaaggcgac ctgcattacg gtcatggcga agcgggcact
    33181 cacgcgccag gcatgagcca gcgccggatg ctggaagtcg aaattgacgt gctggacaaa
    33241 aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg cccgcaagca actggtgctc
    33301 aacctggttt ccagccctgg ttccggtaaa accaccctgc tgacggaaac cttaatgcgc
    33361 ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc
    33421 gcacgcattc gcgctaccgg cacaccagcg attcaggtga acaccggtaa aggctgccat
    33481 cttgacgcac agatgattgc cgacgccgca ccgcgtctgc cactggacga taacggtatt
    33541 ctgtttatcg aaaacgttgg caacctcgta tgcccggcca gcttcgatct cggtgaaaaa
    33601 cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa atatccgcat
    33661 atgtttgccg ccgcctcgct gatgctgctc aacaaagttg acctgttgcc gtatctcaac
    33721 tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat tgaaatcatc
    33781 cttatttccg ccaccagcgg cgaagggatg gaccagtggc tgaactggct ggagacacag
    33841 cgatgtgcat aggcgttccc ggccagatcc gcaccattga cggcaaccag gcgaaagtcg
    33901 acgtctgcgg cattcagcgc gatgtcgatt taacgttagt cggcagctgc gatgaaaacg
    33961 gtcagccgcg cgtgggccag tgggtactgg ttcacgttgg ctttgccatg agcgtaatta
    34021 atgaagccga agcacgcgac actctcgacg ccttacaaaa catgtttgac gttgagccgg
    34081 atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc
    34141 gccggaacag gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac atctctctta
    34201 caccgccgaa cgccctctgc ggattatgga agtgtgcggt ggtcataccc acgccatttt
    34261 taaattcggc ctcgaccagt tactgcctga aaacgttgag tttatccacg gtccgggttg
    34321 cccggtgtgc gtactgccga tgggcagaat cgacacctgc gtggagattg ccagccatcc
    34381 ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg ccggggaaac agggatcgct
    34441 gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga tggatgcgtt
    34501 gaaactggcg caggagaatc caacccgcaa agtggtgttc ttcggcttag gttttgaaac
    34561 caccatgccg accaccgcta tcactctgca acaggcgaaa gcgcgtgatg tgcagaattt
    34621 ttacttcttc tgccagcaca ttacgcttat cccgacgctt cgcagtttgc tggaacagcc
    34681 ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta tcggcactga
    34741 tgcctataat tttatcgcca gcgattttca gcgtccgctg gtggtcgctg gtttcgaacc
    34801 ccttgatcta ctgcaaggcg tggtgatgct ggtggagcag aaaatagcgg cccacagcaa
    34861 ggtagagaat cagtatcgtc gggtggtacc ggatgccggt aacctgctgg cgcaacaggc
    34921 gattgccgat gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag gcgtgattga
    34981 atcttctggc gtgcacctga cgccggatta tcaacgattc gatgccgaag cacatttccg
    35041 cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt tgtggcgaag tcttgacggg
    35101 caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc aaaccgcgtt
    35161 tggtgcgctg atggtttcct ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca
    35221 ggagagtgaa gcgtgaataa tatccaactc gcccacggca gcggcggcca ggcgatgcag
    35281 caattaatca acagcctgtt tatggaagcc tttgccaacc cgtggctggc agagcaggaa
    35341 gatcaggcac gtcttgatct ggcgcaactg atagcggaag gcgaccgtct ggcgttctcc
    35401 accgacagtt acgttattga cccgctgttc ttccctggcg gtaatatcgg caagctggcg
    35461 atttgcggca cagccaatga cgttgcggtc agtggtgcca ttccgcgcta tctctcctgt
    35521 ggctttatcc tcgaagaagg attgccgatg gagacactga aagccgtagt gaccagcatg
    35581 gcagaaaccg cccgcacggc aggcattgcc atcgttactg gcgatactaa agtggtgcag
    35641 cgcggcgcgg cagataaact gtttatcaac accgcgggca tgggcgcaat tccggcgaat
    35701 attcactggg gcgcacagac gctaaccgca ggcgatgtat tgctggtgag cggtacactc
    35761 ggcgaccacg gggcgactat ccttaacctg cgtgagcagc tggggctgga tggcgaactg
    35821 gtcagcgact gcgcggtgct gacgccgctt attcagacgc tgcgtgacat tcccggcgtg
    35881 aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga gttcgcggca
    35941 gcctgcggtt gcggtattga aatttctgaa tcagcgctgc cggttaaacc tgccgtgcgc
    36001 ggcgtttgcg aattgctggg actggacgcc ctgaactttg ccaacgaagg caaactggtg
    36061 atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag cgttacattc ccatccactg
    36121 gggaaagacg cggcgctgat tggtgaagtg gtggaacgta aaggtgttcg tcttgccagt
    36181 ctgtatggcg tgaaacgaac cctcgattta ccacacgccg aaccgcttcc gcgtatatgc
    36241 taataaaatt ctaaatctcc tatagttagt caatgacctt ttgcaccgct ttgcggtgct
    36301 ttcctggaag aacaaaatgt catatacacc gatgagtgat ctcggacagc aagggttgtt
    36361 cgacatcact cggacactat tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc
    36421 gcaactggta aagcgttctg cgctcgccga caacgcggct attgtgttgt ggcaagcgca
    36481 gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac acccccatta aatatgaaga
    36541 cgaaactgtt ctggcacacg gtccggtacg cagcattttg tcgcgccctg atacgctgca
    36601 ttgcagttac gaagaatttt gtgaaacctg gccgcagctg gccgcaggtg ggctataccc
    36661 aaaatttggt cactattgcc tgatgccact ggcggcggaa gggcatattt ttggtggctg
    36721 tgaatttatt cgttatgacg atcgcccctg gagcgaaaaa gagttcaatc gtctgcaaac
    36781 atttacgcag atcgtttctg tcgtcaccga acaaatccag agtcgcgtcg ttaacaatgt
    36841 cgactatgag ttgttatgcc gggaacgaga taacttccgc atcctggtcg ccatcaccaa
    36901 cgcggtgctt tcccgcctgg atatggacga actggtcagc gaagtcgcca aagaaatcca
    36961 ttactatttc gatattgacg atatcagtat cgtcttacgc agccaccgta aaaacaaact
    37021 caacatctac tccactcact atcttgataa acagcatccc gcccacgaac agagcgaagt
    37081 cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc tgctgattaa
    37141 tctccacgag cgggatgatt tagcccccta tgaacgcatg ttgttcgaca cctggggcaa
    37201 ccagattcaa accttgtgcc tgttaccgct gatgtctggc gacaccatgc tgggcgtgct
    37261 gaaactggcg caatgtgaag agaaagtgtt taccactacc aatctgaatt tactgcgcca
    37321 gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg aaatccatcg
    37381 tctgaaagaa cggctggttg atgaaaacct cgccctgacc gagcagctca acaatgttga
    37441 tagtgaattt ggcgagatta ttggccgcag cgaagccatg tacagcgtgc ttaaacaagt
    37501 tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg gcacgggtaa
    37561 agagctgatt gcccgtgcta tccataatct cagtgggcgt aataatcgcc gcatggtcaa
    37621 aatgaactgc gcggcgatgc ctgccggatt gctggagagc gatctgtttg gtcatgagcg
    37681 tggggctttt actggtgcca gcgcccagcg cattggtcgt tttgaactgg cggataaaag
    37741 ctccctgttc ctcgacgaag tgggcgatat gccactggag ttacagccga agttgctgcg
    37801 tgtattgcag gaacaggagt ttgaacgcct cggcagcaac aaaatcattc agacggacgt
    37861 gcgtctaatc gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc gtgagttccg
    37921 tagcgatctc tattaccgcc tgaacgtatt cccgattcac cttccgccac tacgcgagcg
    37981 tccggaagat attccgctgc tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg
    38041 gcgcaatatc gacagcattc ctgccgagac gttgcgcacc ttgagtaata tggagtggcc
    38101 gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgcg gtattgctaa cacgcggcaa
    38161 cgtgctgcag ctgtcattgc cagatattgc tttaccggag cctgaaacgc cgcctgccgc
    38221 aacggttgtc gctcaggagg gcgaagatga atatcagttg attgttcgcg tgctgaaaga
    38281 aactaacggc gtcgttgccg ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac
    38341 gaccctgctg tcacggatga agcgactggg aattgataaa tcggcattga tttaacagat
    38401 gtggttatac tcgatcaaca ttcaatgaaa ggaggcagta tggcagtttc agcaagaaat
    38461 caattaaccg gaacagttag cgcagtagca atgggcgcgg tgaatgacga agtagaactg
    38521 acgctggcag gtggtgcaaa gctggtggca attgtgactc acagcagcca gcaggctctg
    38581 ggtctggcga aagggaaaga agcgattgcc ctgattaaag ccccgtgggt aacgctggca
    38641 acggaagact gtggactgaa attctctgct cgcaaccagt ttgctggcag cgtgtcgaca
    38701 atcaccgaag gcgcagtgaa cgccactgtc catatcaaaa cggatgcggg ttttgaaatt
    38761 gtggcagtgg taacaaatga aagtcaggat gaaatgaaac tcaccactgg ctcacgcgtt
    38821 attgcgttga tcaaagcatc agcaatcctg attgctacca aagcgtaagc gccttatttg
    38881 ccggagagta tggctctccg gcagatttat ttctttggac gatatttttc tggcaactcc
    38941 ggcaccggac gcttgtcatc gatgagatga cgcacggtta agattggatg acgccacagc
    39001 attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa
    39061 cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc
    39121 cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga cgcctgcggg
    39181 catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc
    39241 ttgccggaca tactgcctcc atactcttaa ggtgtattta tactacaact taatttgaaa
    39301 ggggactatg gctctgaagg aggaggttta ttcaaccgat acaattttgc gtacttgctt
    39361 cataagcatc actcaaaagc tgcaaaacag catctttccc ggaaccagca tcaagaactc
    39421 gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct tatgtgatta
    39481 caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca taaccccatg
    39541 agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct caagctgaaa
    39601 gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga actgttttat
    39661 gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg aggtgcttca
    39721 gcgggagagc cgatcccgat ggcggggatc ccctaccatg cggtggaaaa ctacctcgcc
    39781 aaactggtga atcagggcga gtccgttgcc atctgcgaac aaattggcga tccggcgacc
    39841 agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac catcagcgat
    39901 gaagccctgt tgcaggagcg tcaggacaac ctgttggcgg ctatctggca ggacagcaaa
    39961 ggtttcggct acgcgacgct ggatatcagt tccgggcgtt ttcgcctgag cgaaccggct
    40021 gaccgggaaa cgatggcggc agaactgcaa cgcactaatc ctgcggaact gctgtatgca
    40081 gaagattttg ctgaaatgtc gttaattgaa ggccgtcgcg gcctgcgccg tcgcccgctg
    40141 tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg cactcgcgat
    40201 ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg
    40261 cagtatgcga aagataccca acgtacgact ctgccgcata ttcgttccat caccatggaa
    40321 cgtgagcagg acagcatcat tatggatgcc gcgacgcgtc gtaatctgga aatcacccag
    40381 aacctggcgg gtggtgcgga aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg
    40441 atgggcagcc gtatgctgaa acgctggctg catatgccag tgcgcgatac ccgcgtgttg
    40501 cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt acagccggta
    40561 ctgcgtcagg tgggcgatct ggaacgtatt ctggcgcgtc tggcgttgcg tactgctcgc
    40621 ccgcgcgatc tggcccgtat gcgccatgct ttccagcaac tgccggaact gcgcgcgcag
    40681 ttagaaaatg tcgatagtgc accggtacag gcgctgcgtg agaagatggg cgagtttgcc
    40741 gaactgcgcg atctgctgga gcgggcaatc atcgacacac caccagtgct ggtacgcgac
    40801 ggtggtgtta tcgcaccggg ctataacgaa gagctggatg agtggcgggc gctggctgac
    40861 ggcgcgaccg attatctgga gcgtctggaa atccgcgagc gtgaacgtac cggcctggac
    40921 acgctgaaag tcggatttaa cgcggtgcat ggttattaca ttcagatcag ccgtggacaa
    40981 agccatctgg cccccatcaa ctacatgcgt cgccagacgc tgaaaaatgc cgagcgctac
    41041 attattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg caaagcactg
    41101 gcactggaaa aacagcttta tgaagagctg ttcgacctgc tgttgccgca tctggaagcg
    41161 ttgcaacaga gcgcaagcgc gctggcagaa cttgatgtgc tggttaacct ggcggagcgg
    41221 gcctataccc tgaactacac ctgcccgact tttattgata aaccgggtat tcgcatcacc
    41281 gaaggccgcc atccggtggt tgaacaggtg ctgaatgagc cgtttatcgc caacccgctg
    41341 aacctgtcgc cacagcgtcg catgttgatc attaccggtc cgaatatggg cggtaaaagt
    41401 acctatatgc gccagaccgc actgattgcg ctgatggcct acatcggcag ctatgtaccg
    41461 gcacaaaaag ttgagattgg acctatcgat cgcatcttta cccgcgtagg ggcagcggat
    41521 gatctggcat ccgggcgttc aacctttatg gtggaaatga ccgaaaccgc caatatttta
    41581 cataacgcca ccgaatacag tctggtgtta atggatgaga tcgggcgtgg aacgtccact
    41641 tacgatggtc tgtcactggc atgggcgtgc gcggaaaatc tggcgaataa gattaaggca
    41701 ttgacgctgt ttgctaccca ctatttcgag ctgacccagt taccggagaa aatggaaggc
    41761 gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt tatgcatagc
    41821 gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct ggcgggcgtg
    41881 ccaaaagagg tgattaagcg cgcacggcaa aaactgcgtg agctggaaag tatttcgccg
    41941 aacgccgccg ctacacaagt ggatggtacg caaatgtctt tgctgtcagt accggaagaa
    42001 acctcgccag cggtcgaagc tctggaaaat ctcgacccgg attcactgac tccgcgtcag
    42061 gcgctggaat ggatttatcg cttgaagagt ctggtgtaat aacaattccc gatagaaaaa
    42121 tactatcggg aatattatct acagctgacg aatcaaataa aaataccctg tataatagga
    42181 aagtttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat caatgcccat
    42241 cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt attacaatcc
    42301 cgcttacatc aactctcttt tttccccgaa accgacttac ttatttctgt cggcgataat
    42361 attgatcgtg ggccggagag tcttgacgtc ctgcgcctgc taaaccagcc ctggtttatc
    42421 tctgttaaag gcaaccacga agcaatggcg ctggatgcgt tcgcgaccgg cgatggcaat
    42481 atgtggcttg ccagcggtgg tgactggttc tttgagttaa atgatatgga acaaaaagaa
    42541 gctataggtc tattgctgaa atttcatcaa cttccacata ttattgaaat taccaacgac
    42601 attataaaat atgtcatcgc acatgcggat tatccgggca atgaatatct ctttggcaaa
    42661 caaatagcgg agagcgaatt actttggcct gttgatcgtg tgcagaaatc tcttaatggc
    42721 gagttacaac aaataaacgg cgctgattat tttatatttg gacatatgat gcttgataac
    42781 attcagacgt tcgctaacca gatttatatt gataccggat cgccgaaaag cgggcggctg
    42841 tcattttata aaataaagta atgaggccgg gtaaagcaaa gccactaccc ggcacttttt
    42901 attagcgctt accttccgcc agcagcggcg ggatgctcgg caccattggc gcgtcatcaa
    42961 tatctttctg cgtcatgcgg aacgcttcgg gataatgttc gcggctggta cggcgcagcg
    43021 gttcggtatc gcgccaggta taaaggcaat gctggcactg atataccgtc cagacatctt
    43081 tcaccggcga tttcgccatc acttcaatct gttcatcggc acaacgtgga caaatcatct
    43141 tgttctcctt atttacgtgc ggccagcata gcggtcagtt tttcagccca ggctttggtt
    43201 tccggcaaat ccaccaccgg ctggctgtag tgaccacggt tgtccggggc gacaggcgtg
    43261 gtggcgtcga taatcagctt gtcggtgatc cccgccgggc ttgagcctgg gtcgagttcc
    43321 agcacggaca tattcggcaa ctgcaccaaa tcccctgccg gatttacttt cgaggagagc
    43381 gcccacatca cctgcggcag gttgaacggg tcaacgtctt catcgaccat aatcaccatc
    43441 tttacgtagc ccagaccgtg cggcgtggtc atcgcacgca ggcccaccgc gcgggcaaag
    43501 ccgccgtagc gttttttggt ggagataatc gccagcaggc cgtgggtgta catggcgttt
    43561 accgcctgca cttccgggaa ctcggctttc agttgctgat acagcggcac acaggtggct
    43621 ggccccatca ggtagtcgat ttcggtccac ggcatgccga ggtacagcga ttcgaaaatc
    43681 ggcctggtgc ggtaagagac tttatcgatg cgcaccacgg tcatgttgcg cccgccggag
    43741 tagtgcccgg taaactcacc gaacggcccc tcgatttcgc gtttacggct ttcgatgacc
    43801 ccttcgagga tcacttctga accccacggc acatcaaaac cggtcagcgg ggcggtggcg
    43861 atcgggtacg agctttcacg cagcgcgcct gccatttcgt actcagattg atcgtatttc
    43921 agtggcgttg cgcccatcag cgtgatgatc ggatcgttac ccagggtgat ggcaatcggc
    43981 agatcttcac cgcgctcttc tgctttatgc agatgcaggg cgatatcgtg catcggcacc
    44041 ggttgcaggc cgagcttacg cttgcccttc acttccatgc ggtagatgcc gacgttctgc
    44101 ttgccgaagt tatccgggtc gagcggatcg cgggaaacca cgcacgcttt gtcgagatag
    44161 aaaccgccat caccgtcgtt taaacgaaac agcggcagga tatcgaacag gttgatctcg
    44221 tcgccatcga cggtgttctg cgcccaggct ggattggcgc ggcgctccgg ggcgatcggg
    44281 aagttatccc agcggcggat aaactcatca atctgctttt tgaccggagt gtttggcggc
    44341 aggccgaggg aaatcgcgtg gttctgccag gaaccgatgg tattcatcgc cacgcgggca
    44401 tcggtaaaac cgcgaatatt atcaaaccac agcgccggtg caccatcgcc gatacgcccg
    44461 gtggcgttgg cagcggcagc cagatccggt tccgcgttca cctcttcgct gattttcagt
    44521 aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta aatcatcaaa tgccattatt
    44581 cattctcctg agaaaaattc cgggcctgcg gcaatccttg ccagcgcctg gcgtggggat
    44641 gttcgaggcc aaattgatcc agcacgcggg ctactacatg gtggacaatg tcatctaccg
    44701 tttcgggatg gttataaaag gcaggcatcg gcggcaccat cgccacgccc atgcgtgaaa
    44761 gtgcgagcat attttcgaga tggatggtgc taagcggcat ttcacgcggc accagcacca
    44821 gtttgcggcc ttctttgagc acgacgtccg ccgcgcgccc taccaggcca tcagcgtaac
    44881 cagcgcggat accggcgagc gttttcatac tgcacggaat aacgatcatg ccgtcggtac
    44941 gaaaggaacc tgaggagatg gtcgccgcct gatccgccgg gttatggctg aagtcagcga
    45001 gggcagcaac atcgcgggcg ctgtaaggcg tttccagttc aatggtggtt ttcgcccact
    45061 tcgacatcac cagatgagtc tcgacattcg gcatctcccg cagcgcttgc agtaatgcca
    45121 caccaagagg cgcaccggta gcccctgtca tcccgacgat cagtttcatt gcttattctc
    45181 ttaattgttc gtatacgaac gttattatta aaatacgcct ctgttctcat cgcggcaagc
    45241 actgaaaagt aacaacatcc ccttcgtcac tgaaaaaccg ctatgatagc ggcagagaat
    45301 tagcccggag tttacatggc gttacgaaat aaagcgttcc atcagttacg acagcttttt
    45361 cagcagcaca catctcgctg gcagcatgag ctacctggcc tgaccaaacc gcagtacgcg
    45421 gtgatgcgcg ccattgctga taagcctggt atcgaacagg ttgcgctgat ggaagcagca
    45481 gtcagcacca aagcaacgct ggcagaaatg ctggcaagaa tggagaatcg tggcttagtc
    45541 agacgtgatc atgatgccgc tgacaagcgg cgtcgctttg tctggctgac tgccgaagga
    45601 gaaaagatac ttgctgcggc gataccgatt ggtgacagcg tggatgagga atttttgggg
    45661 cgtttgagtg cagaagagca ggagctgttt gtgcagctgg tgcgcaagat gatgaacaca
    45721 taggatgcaa attgccgggt aggacgctga cgtgtcttat ccaggcgaca aaaacacaag
    45781 cttcccaaac aaaagaaaaa ggccagcctc gcttgagact ggcctttctg acagatgctt
    45841 acttactcgc ggaacagcgc ttcgatattc agcccctgcg tttgcaggat ttcgcgcaaa
    45901 cggcgcaggc cttcaacctg aatctggcga acacgttcac gggtgaggcc aatttcacga
    45961 cctacatctt ccagtgttgc cgcttcgtac cccagcaaac cgaatcgacg tgccagtact
    46021 tcacgctgtt tggcgttcag ctcgaacagc catttgacga tgctctgctt catatcgtca
    46081 tcttgcgtgg tatcttccgg accgttctct ttttcatcgg ccaggatgtc cagcaacgct
    46141 ttttcggaat caccacccag cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc
    46201 atacggctga cgtcatcaac tggcttatcc agttgctctg cgatctcttc cgcacttggt
    46261 tcatggtcca gcttatggga caactcacgt gcggttcgca ggtaaacgtt cagctccttt
    46321 acgatgtgaa tcggcaaacg aatagtacgg gtttggttca taatcgcccg ttcaatcgtc
    46381 tggcgaatcc accaggttgc gtatgttgag aagcggaaac cacgttccgg gtcaaacttc
    46441 tctaccgcgc ggatcagccc caggttgccc tcttcgataa ggtccagcaa cgccagacca
    46501 cgattgccat aacggcgggc aatttttacc accagacgca agttactctc gatcatccgg
    46561 cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc
    46621 gttaacagtg gtgaataacc aatctcacca aggtaaagct gagtcgcgtc caacacacgc
    46681 tgtgtggctc cctgcgataa cagttcctct tcggccaaat cgttatcact gggttcctct
    46741 tctactaagg ccttttcgtc aaaaacctca actccgttct catcaaattc cgcatcttca
    46801 tttaaatcat gaactttcag cgtattctga ctcataaggt ggctcctacc cgtgatccct
    46861 tgacggaaca ttcaagcaaa agcctggttc cgccgattta tcgctgcggc aaataacgca
    46921 gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc
    46981 cggtgctacc catggttgct attttttgcc ccgccttcac ttcttgttgt tcccggacca
    47041 gcattgtgtc gttatgggcg taggcactca ggtaatcatc attatgtttg atgataatca
    47101 gattaccgta gccgcgcagc gcgttaccgg cataaacaac gcggccatct gcggtcgcga
    47161 taattgcctg tcctttgctg cctgcgatat caatcccctt gttgcccccc tcagaagcgc
    47221 caaaggtttc gatcactttg ccctcagtcg gccagcgcca ggtggagata ggcgtactgg
    47281 ttgatgtact gctgacagtc ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg
    47341 tgaccgtggt cgcagttggc ttgttgttcg gcaacatttt gttagcactc tgttcacccg
    47401 aagactcaga atacgtaatt gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag
    47461 gcttgatcac aactccttgc tctgctgcgt cggcctgggt aatggcattt ccgccagtga
    47521 ttggcgtacc ggaagcattc cccacctgta aggtctgacc aacgttcagc gcgtatggtg
    47581 cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat
    47641 agaaaagtgt gtcgcctttt ttcacggtgt atgtactgcc gctataactg cctttcggaa
    47701 tgttcccata ctgacggtta tagacgatgc gtccgttttc catctgtacc ggctgctgag
    47761 ctactggctg cactggctgg atttgcggtt gttgagtagc ctgaatttgt ggctgctgta
    47821 ccggctgaat ttgcggttgc tgcgctgtag acgtcgtccc cattttcggc ggcggcgtaa
    47881 tcaacatacc agaattggta tttgcaggcg cattgccatt gacggaactg accggtgccg
    47941 gtggatttga agtgtcagaa cagcctgcca gccatagcga aaccagtgac aaagccgcaa
    48001 tgcggcgaac ggtgaatttt gggcttcccg cgctcattta tcccccagga aaaattggtt
    48061 aataaccagt gacataatta ccgtgcaagg caccatactg aacactggaa aagatgttca
    48121 cgatacgctg acctgcggca aaataaccag gaaaaatcca ggtatttcct cacgttttaa
    48181 gccagctcac ccttcactaa agggacaaag cgcacggcct ccacggtatc gataataaat
    48241 tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct cctcccctac gggtaagacg
    48301 agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc
    48361 gtaacaatga tagcgtcaaa cggcgcacgt gcctgccaac cttgccatcc atcgccatga
    48421 cgggttgaaa cattatgtaa atcaagattt ttcagacggc gacgcgcctg ccactgcaaa
    48481 cctttaatcc gttcgaccga gcaaacatgc tggacaagat gcgccaggat tgccgtttga
    48541 tatcccgaac cggtgccaat ttccagcacc cgcgactgcg gcgtcaactc gagtaattcg
    48601 gtcattcgcg ccaccatata cggctgcgaa attgtctgcc cctgacctat cggcaaagcg
    48661 atattgtccc aggctttttg ttcaaacgct tcatcaacga atttttcacg cggcacggcg
    48721 gcaagtgcat tcagcacctg ctcatcctga ataccttgcg cacgtaattg atccagaagt
    48781 gcttgtacgc gtctgcttac cattgcgtgc caactcccac gctgtttaac cagtctgaaa
    48841 ccacatcttg cgcgctatgc gcagttaaat ccacatgcag cggcgtgatg gagacatagc
    48901 cctcatctac cgcagcaaaa tcggtccccg gaccggcatc acatttaccg cccggcgggc
    48961 caatccagta cagcgtattg ccgcgcggat cttgctgcgg gatcacctgt tctgccggat
    49021 gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg atccaagggt aaatccggaa
    49081 cgttaatatt aagaatacgc ccggtgcgca gcggctcttt acacagtgcg cgcaaaattg
    49141 aacaggttac cgccgcggca gtgtcgtaat gtttatgccc gtcaagcgag acggcaagcg
    49201 ccggaaaacc taaatgacgg ccttccatcg cggcggctac cgtaccggaa taaataacat
    49261 catcccccag attcggcccg gcgttaattc cggacacaac aatgtccggg cgcggacgca
    49321 tcagagcatt cacgccaaga tagacgcaat cggtcggggt tcccatttgc acagcaatat
    49381 caccattttc aaaggtaaac gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc
    49441 cgctgcggtt acgatcgggg gcgaccacct gaacgtcagc aaactcacgc aaggctttcg
    49501 ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt actcagcaat atgcgcataa
    49561 tcacctgttg tgttgataag ttccctgaca acgctggttg caaaactacc cgccggaagc
    49621 cagaaacgga tctctacggt gacgtcatcc caccagttcc agcttaattg ttgcggatac
    49681 agcagcatcg ctctgcgcgc ggcttcaact ttttcgcgca ccagtaaggt ttgtaattca
    49741 gtttctgcgg cgaccgctgc ttgttcgaat gccaacgctt cacgctgagt tccccattcg
    49801 ccactgcccg gcaatgcggc ggttatcatc agctctttat cgttgacgcg acgctgtaat
    49861 tccaccagtt cttcggtggt tgcaacaaac cagctaccac gtccggctaa ttgtagcgca
    49921 tcgccgtcaa caacttgatt aacgtctgct tttttgaggc gctcagcaac aatctgatta
    49981 aacaacgcac tgcgggctgc cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga
    50041 gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc taccgccaat cccaaaacgt
    50101 tgggcaccga agtagttcgg tacacctttt acgcaaatat cgatcagacg ttgttcaacg
    50161 tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg cgttaccttt cagcgcccct
    50221 aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct ggcagccttc caattgaaag
    50281 gcgctcagat cgggcatttc cttgcccggc acgcgagcgc ataaccactg ttctgtaaca
    50341 gcatgtttgt ctttttgccc agcaaagctg acttcacggg catgaatttt caggaatttc
    50401 gccagtgcat ccgccacaaa acgggtattg cagccgtttt tgaggattct aaccagaata
    50461 tgctcacctt caccatcagg ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga
    50521 ttggctttca gcagcccggt gccttgcggt ttaccgtgga ggtaagtgag attatcaaac
    50581 tcaatcattt tgttgcctta atgagtagcg ccaccgcttc acaggcaatc ccttccccac
    50641 gtccggtaaa tccaagtttt tccgtagtag tggctttcac gttaacatca tccatatggc
    50701 agccgagatc ttcggcaata aacacgcgca tttgtggaat gtgcggcaac atcttcggtg
    50761 cctgagctat gatagtgaca tcgacgttgc caagggtata acccttcgcc tgaatacgac
    50821 gccaggcttc gcgtagcagc tcgcggctat cggcaccttt aaatgccgga tcggtatccg
    50881 ggaacagttt gccgatatcc cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat
    50941 ggagcgccac gtcgccatca gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc
    51001 caccaatgat aattgggcct tcaccgccaa aggcgtgtac gtcaaaaccg tgtccaattc
    51061 gcattatgta ttctcctgat ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc
    51121 ctccgggcgc gtgactttaa tgttatccgc acggccttcg accaactgag gatggaatcc
    51181 gcaatattcc agcgccgagg cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt
    51241 cagacagtca tgtaacagct cacgagggaa aaattgcggc gtcagcgcgt gccataagcc
    51301 gttgcgatca acggtatgag caatggcatt tttgcccggt tcggcacgtt tcatagtatc
    51361 gcgcactggt gctgccagga tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg
    51421 cgcgaggtca tcctgatgca gacaaggacg agcggcgtca tgcaccaata cccactgcgc
    51481 gtcgccagcg gctttcaaac ctgccagcac ggaatcggca cgctcatcac cgccatctac
    51541 aacggtgatt tgcggatgat tcgccagagg aagttgtgca aaacggctat cgccaggact
    51601 tatggcaatg acgacacgtt tcacccgggg atgcgccagc agcgcatgca ccgagtgttc
    51661 aagaatggtt tgattaccga ttgagagata ttgcttagga cattccgttt gcattcgacg
    51721 gccaaatccg gccgccggaa ccacggcgca aacatccaaa tgagtggttg ccatgttaat
    51781 tcccgggctg atttatcgat tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg
    51841 taccagacga taaaaagttt cgcccggcct ggtcatgctg agttcattac gcgcacgctc
    51901 ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg
    51961 cgctttaagt ttcgcgtttg tagcttgctg tgccgccaca tcatcattga cgcgggtata
    52021 gtcatgtata ccgttcttac cgaaccacag cgaatactgt agccagacca gaatagccag
    52081 caacagcagc gttagtttac ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca
    52141 tccgaagacg actctacatc ctctgttggg gataccgcga caacgcgggc aaatgtacca
    52201 catttgtcca ttgttacgta tacccagggc gtgcagaaca taatctcatt attagttacg
    52261 gtttgaatta tgaacagagg agacaagaaa gtacaaatta gcccagtagc cacataaaca
    52321 gtgcgccaaa cataatgcct actgtcatca gggtgaaaac aatactgtag cgtagctttc
    52381 cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc aacgggcatc agcgccagaa
    52441 agaaaggcca ggtgtagata aagaagaaca gcgtgttaga gccataaatc aacatcggca
    52501 tcgccagcgc aaataaccaa gagataaaac cgaccacggc accaggcagt gaccatgtgg
    52561 tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt aatgttatgg ctattacgca
    52621 tatttgatcc tgttactttg acgaaccggg catggaaacc cggtggtgtc tcaggatctg
    52681 ataatatcgt tctgtctcaa cagatctaat aattgctgta ccaaatttgt tactaattgt
    52741 tcaccattga gatgaatttc tgccgattca ggcgcttcgt aaacggaatc tattcccgta
    52801 aagttgcgca gttcaccggc acgcgctttc ttatataagc cttttggatc gcgggcttcg
    52861 caaatcgcca gcggcgtatc gacaaacact tcgataaagc gcccttctcc tacgcgttcg
    52921 cgaaccatct ggcgttcggc gcggtgtggc gagataaatg cggtcagcac caccagtccg
    52981 gcttcaacca tcaaattcgc cacttcaccg acgcgacgga tattctcttt acgatcggca
    53041 tcgctaaaac cgagatcgct gcataatccg tggcgaacat tgtcgccatc cagcagatac
    53101 gtactgacgc cgagtttatg taacgcctcc tccagcgccc cggcgaccgt tgatttaccg
    53161 gacccggaga ggccggtaaa ccacagcact acaccacgat gaccgtggtg tagctcgcgt
    53221 tgttgcacag tgaccggatg gctatgccag acgacgtttt cgtcatgcag cgccattatt
    53281 tatcccccag caaatcgcgc gcgccccagt gtggaaagtg gcggcgaacc agggcattca
    53341 attccagttc gaatgcacta aattcagatg gcgcagcagt tgcctggcta actggctcat
    53401 gcaccatacc ggcacctacg gtcacattgc tcaggcgatc gataaaaatc agcccaccgg
    53461 taaccgggtt ttgctgataa cgatctaaca ccagtggctc gtcaaaagtg agatccacca
    53521 ggccgatgcc gttcagcggc aggttttcaa cttcgcgttg ggtaaggtta ttaatatcaa
    53581 cctgataacg aatgccatca acacgagcac gcgtcttctt accggcaatt ttgatgtcgt
    53641 aactctggcc cggggaaagc ggctgttccg ccatccatac cacatccacc gacgcgctct
    53701 gcacagctgg taacgcttcg tctgccgcca gcagcagatc gccacggctg atgtcgatct
    53761 catccgtcag caccagggtg atagcttctc cggcaaaggc ttcttcgcga tcaccatcaa
    53821 aagtcacgat ccgcgcgacg tttgattcca caccagaggg cagcactttt acacgttgcc
    53881 cgacttccac gcgaccggat gccagcgttc cggcgtagcc acgaaaatcg agatttgggc
    53941 ggttaacgta ctgcaccggg aagcgcattg gctgggcatc caccactcgc tgaatctcca
    54001 cggtttccag cacttcgagc agtgtcggac cgctgtacca cggcatactt tcactttgcg
    54061 aagccacgtt gtcaccttcc agtgcggaga gcggcacaaa gcggatatcc agattacccg
    54121 gcagctgccc ggcaaaggtc agataatctt cacgaatacg ggtgaacgtc tcttcactgt
    54181 aatccaccag atccattttg ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg
    54241 agataaaact gtgacgacgg gtttgatcga gcacgccttt acgggcatcg atcagtaaga
    54301 tcgccagttc acatgtcgat gcgccagtcg ccatattgcg ggtgtactgc tcgtgccctg
    54361 gagtgtcggc gataataaat ttacgcttct cggtagagaa atagcggtag gccacgtcaa
    54421 tggtgatgcc ctgttcacgc tcagcttgca ggccgtccac cagcagagcc agatccagct
    54481 tttcgccctg ggtgccgtga cgcttactgt cattatgcag cgatgagagc tgatcttcat
    54541 agatttggcg ggtatcgtgc agcagacgac caatcagggt acttttgccg tcatcgacgc
    54601 taccacaggt cagaaaacgc agcaggcttt tatgttgttg cgcaatcatc caggcttcga
    54661 cgccgccttc attggcgatt tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga
    54721 cgttttttca gctccataga accagcctgg tcgcggtcaa tcacgcggcc ctgacgttca
    54781 ctggtggtgg aaaccagcat ctcttcgatg atttccggca gtgtttgtgc atttgactcc
    54841 accgcaccgg tcagcggcca gcagcccagc gtacggaaac gcaccatccg ttttttgatc
    54901 acttcgcccg gttgcaggtc gatacggttg tcatcaatca tcatcaacat accgtcgcgt
    54961 tccagaaccg gacgttccgc agcgagatac agcggcacaa tgtcgatatt ttccagccag
    55021 atgtattgcc agatatcctg ctcggtccag ttagagagcg ggaagacgcg gatgctttcg
    55081 cctttgttaa tttgcccgtt gtagttgtgc cacagctccg ggcgctgatt tttcggatcc
    55141 cagcgatgga agcggtcacg gaaagagtag attcgctctt tagcacggga tttctcttcg
    55201 tcacggcgcg caccaccgaa ggcggcatca aaaccgtatt tattcagcgc ctgtttcagg
    55261 ccttcagttt tcataatatc ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc
    55321 atcgccacgc cttccgggtt tttatgcacc agcagctcgc agccgtaggc tttcgccgta
    55381 cgatcgcgga actcatacat ctcgcggaat ttccagccgg tatcaacatg cagcaacggg
    55441 aaaggcagcg tacctggata aaacgccttg cgcgccagat gcagcatgac gctggaatct
    55501 ttaccgatag agtagagcat caccggattt gaaaattctg ccgccacctc gcgaataatg
    55561 tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc gtttttgatc cataaccgtt
    55621 cctttgcaat accgctattt tcttgccatc agatgtttcg actataggga gcgtaagaga
    55681 acgaatgaaa ttaccaatta gaatgagtag ttccttaacg gaataacgat ttggcaaagc
    55741 taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt
    55801 ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat ttgcatggta tttaaggact
    55861 cactatgttt tccgcattgc gccaccgtac cgctgccctg gcgctcggcg tatgctttat
    55921 tctccccgta cacgcctcgt cacctaaacc tggcgatttt gctaatactc aggcacgaca
    55981 tattgctact ttctttccgg gacgcatgac cggaactcct gcagaaatgt tatctgccga
    56041 ttatattcgc caacagtttc agcaaatggg ttatcgcagt gatattcgga catttaatag
    56101 tcggtatatt tataccgccc gcgataatcg taagagctgg cataacgtga cgggaagtac
    56161 ggtgattgcc gctcatgaag gcaaagcccc gcagcagatc atcattatgg cgcatctgga
    56221 tacttacgcc ccgctgagcg atgctgacgc cgatgccaat ctcggcgggc tgacgttaca
    56281 aggaatggat gataacgccg caggtttagg tgtcatgctg gaattggcag aacgcctgaa
    56341 aaatacgcct accgagtatg gtattcgatt tgtggcgacc agcggcgaag aggaagggaa
    56401 attaggcgct gagaatttac tcaagcggat gagtgacacc gaaaagaaaa atacgctgct
    56461 ggtgattaat ctcgataact taattgttgg cgataaattg tatttcaaca gcggtgtaaa
    56521 aacccctgaa gcagtaagga aattaacgcg cgacagggcg ctggcaattg cgcgtagtca
    56581 tggaattgcc gcaacgacca atccgggttt gaataaaaat tatccgaaag gcactgggtg
    56641 ttgtaatgac gcagaaatat tcgacaaagc gggcattgct gtactttcgg tggaagcgac
    56701 taactggaat cttgggaata aggatggtta tcagcaacgc gcaaaaacac ctgccttccc
    56761 ggcgggaaat agctggcatg acgtaagact ggataatcaa caacatattg ataaggcact
    56821 tcctggaaga atagaacgtc gctgccgtga tgttatgcgg ataatgctac cgttggtgaa
    56881 ggagctggcg aaggcgtctt gatgggttgg aaaatgggag ctgggtgttc taccgcaggg
    56941 gcggggaatt ctcagtgata tccatcatcg catccagtgc gcccggttta tccccgctgg
    57001 cgcggggaac acatattttt gtcggtcgcc tggaagttca gtttcggttt atccccgctg
    57061 gcgcggggaa cacggattca gaacgccgtc atcctccgca atccacggtt tatccccgct
    57121 ggcgcgggga acacaattca tcatcagatt cacggtcagc gccaggcggt ttatccccgc
    57181 tggcgcgggg aacacatcca gcgcgaccat ttttccctgt aaattttcgg tttatcccca
    57241 ctggcgcggg gaacacctat cttgcgacag ctaaatattc caacaaagcg gtttatcccc
    57301 gctggcgcgg ggaacacaaa gtgctgaagg cgcttaatgc tctcgctggc ggtttatccc
    57361 cgctggcgcg gggaacactc atacagcagg gtgccgtcac cgtcacgcaa cggtttatcc
    57421 ccgctggcgc ggggaacact tgcaatctga tggggcagct ttcttcaatt tcggtttatc
    57481 cccgctggcg cggggaacac actaagcata catatctgtt tttaaacaac tttattccac
    57541 atcaacaatc taccaactaa attcaaacat ttccttattt ttaaagaaca cataacctat
    57601 tgattatcaa caggaagaaa agaaaccaaa cgtaacccat ccaaatccac cggaatacgt
    57661 ctgttttctc cccaggtctg aaattcaaaa cccgactcgg tattggtcgc ccaggccatc
    57721 accacatttc cgcaaccagc cagttgggta atttgctgcc agatcatctc ccgaatacgt
    57781 tttgatgtat caccaacata cacaccggca cgcacttcca gtagccagat tgcgagccgt
    57841 ccacgtaagc gcggagggac attttctgta acaaccacga ccatgctcat ccgccgcgcc
    57901 cccggtgacc actatcaccc agcgtttcag gttcagggat ggcaggcggt aacatatccg
    57961 gcgcgggttg tggtggttca atttcacctg cagcaaggac ttcctcaatt aacggtatta
    58021 atttgcccgt taacttagtg ctacggaaaa tatcgcgaca ggctaatctg acttctttat
    58081 caggttctgc gggttgcctc gctgctattt caaatgcctt tggcacaacc gaatcaaatt
    58141 taatgatatc ggctatgtca taaacaaatg aaagcggttt gccactatga atatatccaa
    58201 tagcgggcgc atatcccgcg gctaatactg ccgcttcaga aataccgtac agacatgatg
    58261 tggcagcact gatgcagcga ttcacaacat cgcctttttc ccagtcttta ggatcgtatt
    58321 tgcgaccatt ccatttcaca ccatattgtt tcgccagtaa tgcataggtc tggcgaacgc
    58381 gggatccctc aattccccgt agctgatcca ctgaacggcg agctggcggt ggctcacgaa
    58441 aacgtaattc atacattttg cgcaccacct tcaggcgtag atcttccgtt aaagccagct
    58501 ttgcctggta gagtaattta tctgcccgcg cccctccggg ttgtccggaa gagtaaacgc
    58561 gaacgcccgc ttcaccgacc cagaccagca gtgttcccac cgtggcggcc agatgcaccg
    58621 ccgcgtggga aactctcgtt cccggttcga gcataatgca ggcgaccgat cccaccggaa
    58681 tgtgcgtgcg gatcccggtt ttgtcgatca gcacgaaagc gccgtccagt acgtcgattt
    58741 gaccgtactg gaggaagatc atagaggtgc gatcttttaa cgggatcgga ctcagtggta
    58801 caaacgtcac acctctgctc cgggtttgat cagcatcaga ccacagccga acgcgcggct
    58861 tttgccatac ccctgactga gacgttgcag aaataacccg ggatcggtga ccgtaagcat
    58921 ccccgtataa tccacgctac tgaactggat cagttgccgg gagttttccc gccgcagttg
    58981 ctgttgtcgg taggcatcaa ccgaggtatc cagcagtgta aaaccgcttc tctccccctg
    59041 cgctgccagc cagtccagcg ccgcctgttg ttgatgcaac cagacatcac gcccttccgc
    59101 ctgtcccctc acctgccgtt tcgcctccat cagcagatcg tggcgcttgc ccgctttaca
    59161 aattgtggga ttcgcccgca ggttgaaaca aagggattgt ccggtacgca gctccggagc
    59221 aaatgaccgg cattcgatag tgaaagtttc actttccgcc gggcgttcct gcgagaggac
    59281 aaaaaagcga aacgcaccct gaagttcctc ccgacgataa agaaattgcc tttctttgcc
    59341 gctagggaag agatcccaca gccactgatg catcacatat tcaccgcgat ccaccagatg
    59401 cagcaactgc gcaggcaaaa gctgagcggt atgtaaggtt attcttgaga ggtacatggt
    59461 tcctccttgc tgagccacgg cccctgattg acggtgcgtt ccccaaacag ccactgctgg
    59521 cgatttaaag gaacatcccg gcggcgtaat attttgctca cgaccaggcc gtcgtgctcc
    59581 ccttcccacc agcattcatc ctgaagtttc gggagtgaga ctttcaggtc gtggaaatga
    59641 tcctgatact gttggtatgc gttacgtaaa acatcagacg cgttgccttc gagcagtaac
    59701 ggcgcaagtg gtaatgccag cggatgactt tttcgcccca gataaagagg aaaaactgga
    59761 tggcgtaaac cgccctgcaa ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc
    59821 gccaccatcc accaggcgtc ggtgtagtag tcgcgccggg agataatcgc gctcagaaga
    59881 tcagggtcgc tcaactcttc gcgacggctg aaataacgcg ctttacgcac ctcttttggc
    59941 atctggatcg tgtgataatc ccgtgcccag cgcgggttac ggctggcgca aaccaccagt
    60001 gaatagtggc gattaaacgc gtttaagcgt tcggtatcat cacgccgaat ccctaccccg
    60061 gcggccagca gacccagcaa tgctgagcgc gaaggcagtt catgggtatg acgcacttcg
    60121 ccgggggcat cgacgcccca ggatgccatt ggcccatgaa gctgaaaaat cagatattgg
    60181 ctcataagcc gccccttacg cgcagataaa gtccagcacg tccttcatgc ttccctgttt
    60241 gttcatcacg tcaaaacttg cgcattcggt cttctgttcg tagaccgtat tcatattttc
    60301 gcgaagcgtt gtaatacgct gcaccgccac gtctaactgc cgggtgccat taatgggttc
    60361 atagaaggcc gcagccagag aacgcggttg atcggtgcct ttttccgcca gcgcccagga
    60421 agcataggca cggctggcaa agctattttg tttaccggtt ggggaaactt taagcgcggc
    60481 ttccgtaaaa gcgcgtatgg tctgattagc taacgcttcg tcaccgccga ggttttctac
    60541 cagcagatct ttatcaatgc agatataggt gtagaaaagt gctgaaccga atccggtttc
    60601 tccgagatgc ccggcaccgg catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa
    60661 aaaatcatct tcgacaatcg tttcactgac accaaacgca tgcgcgacct ggcaggcggc
    60721 ttcaacatta aactcaggtt tattcgccag catacgacca aacatagcga tatctaccgc
    60781 tatacggtct ttacgtaaca acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc
    60841 cagttgatgg gccagcgctt ttacggcgtc aaattctgcc gggctgatat gaactaattg
    60901 ttcagtttcg gcgttagtga gcggatcttt tggttttttg tcgtttttag ctttcccaag
    60961 ataatccgca atttttgccg cccattcgat ggcttttttc tcttcgattc ctttctcaat
    61021 caggatagtt gccgcctcac gcgcaattcg cccactgcga ataccaatat ggcccgccag
    61081 tgcctgttca aaaagtgcag aagtgcgcca cgcacgtttc agactttgtg aggaaacgcg
    61141 cagacgcgtt gcaccaccca gtaccacagt ttttggcgca ccagtatcat cacggttaag
    61201 gttggcagca gggtaagcgg ttaacaaatg aagctgaata aatgttgtca tgagatagtc
    61261 ctttattaat tttgttcgtt atcggcgtcg ccagcctggt aatattccag tgcccagcgg
    61321 atacggataa attcggttgg ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc
    61381 tcctggcacc agcggaaaat atcttctgcc aaagagataa gattgacgga accgtttaat
    61441 aatttgaccg cgcgacgtaa ctgacgcagt aaatcatccg gcgtttttac tgccgacagg
    61501 cgagtaaaac gcccctccga cattaccgcc gccagctgcg cggcaaaagg ctgccgttca
    61561 tcaatggctt ttacattggc gctaactgcg gcgaccagcg ccagtgcggt aaaacgccac
    61621 tcttgtgctt caattttcca caaagtgtgg gtctgcatca gcagagaacg aaacccttct
    61681 gacaagcaaa catcattaac cgttgtgcta cgccgcagac tggcacgatt gccgcgttta
    61741 gcctgcaact cttcgtgcca tttacgcagc gtggctttgt gctcctcttt tacaatactc
    61801 attcagcagc ctcctgcttt ttctccctgg cggctttagc attttgcttc tccgccgatg
    61861 ttgtaaaata tttcttgcgt gcggtcatga cgcgtttcaa atcaacgggc tcataaggat
    61921 tggtgaatac acgctcgtca aaatcctgac gcgcaaataa ccaaatttcc ttttgccatt
    61981 tgccgagtaa ttcatctgga tcctgacctt cttcaatttg gcgcactaac ctcaggaagc
    62041 gatgctgagt tttgttccag aagtcgatat ccacaaaact gaaatcaccc cgtgcacctt
    62101 ttggatcgga gaaccacgct tctttcaatg cactccgtaa cagactcaga atccgtgaag
    62161 ccgtttgcgc agccagccgc agtttcggta tctggccttc ttttttattg agcagaagcg
    62221 ggaaatggtg ctcgtaccaa cagcgcgctt tcatgttgtc aaaatcataa ccaaatcccc
    62281 acaggcccac ttttgcctgt ttcagactgc tggcattaaa gagtttcacc accagcgcgg
    62341 gaagttccgt attgttttct gatttccccg tttcgataag gcctaaccag tcgcgccaga
    62401 ttaaaccgcc cggttgtggt ttaactgagt aaaactcgcc gccctcttta agtggtacac
    62461 ggtaaggcgt taagggatgc tgccacatgg catagttagc gccgtaattt ttggtcgtca
    62521 tcaggctcag aagcgcgtcg ctctgctcac cgcaaatatc gcagttgccg actgtggtgg
    62581 tattaaaatc aatacgaata cgccgtggca ttccccagta cgcctggagt ttattaacct
    62641 gatcatcggt taccaccgca ccggccagtt cgctggtacg cgtcgggcca agccagggga
    62701 aaaccagatc gtcaaatttt tggggtagcg gtaagtcggc ttcatcctgc ggcatcacgt
    62761 tgagccacag tttgcgccat aagggtgttt gttgattgcc ctgatattcc tgtaattcaa
    62821 tcagagtggt catcggccca ccgccgcgca aaccggtgcg atagcctttg ccacccgctg
    62881 gtgcattcaa ctgtaaagag aacaaggcca atgctaaaca atgggggcac agatattccg
    62941 ttacaccacg cttgataaaa tggtctttat taaacttcgt cgtttgagcg ccgggaacat
    63001 caggcagtag cgaagcgacc tgaactttat ctcccgtgag cgcctcaaaa tcctgcataa
    63061 atgaagggga atctgggcca aactggaaag catgttctaa tgacagcaat gcttccctta
    63121 gcttttcagc ttccagtccg tcttcccata tatcatccca acgacgatga tcttttggcg
    63181 caaagctggt ttgtagtagc cccagcaaaa actgccatgc cgcaccttgt aaatctgctc
    63241 gcggcgcggc gatatcgaca acattttcat cagccagatc gactggtgcc agcttgcctg
    63301 tcgttccgtc tttataacga acgggcaacc acggggttgt cagaagtgaa aacgagttca
    63361 tacgcatcct ctcgtaaaaa gaccatccct ggtcggttgt ccatccttcc atccttttcg
    63421 gtgtatcaac tattcagaga gccctgtcgc aggcatctca accaaccttg ccagtcaatc
    63481 cttccatagc cgaatacccg caactttcgt catcggtgac taaaatcacg tttgccatct
    63541 ccggctcttg ccgctgttca atgcaccact gcctgaacgc atccccttcc agtaaagaaa
    63601 actcatcccg atgttttttc caccagcttc gacgcactct gacaacgctc atttcccatg
    63661 cgtgagcacc ggtagcataa ggcttcacca caccgtcaat acaggtagcc agccacaggg
    63721 aaacagactc ttcagccaga cgtgtcgaca gtttttccgg aaggtaatcg ttgatactgg
    63781 cggcatagcc gggcctgaag ttcagcacaa actttttagc cattgtgcga tcgcagtaat
    63841 atttgcccac ttgctcctgc tcgctgcggg caaatccttc cggcattacc acgtcctcac
    63901 cgtaaactga ttcaataaga agccgggctg cgtgtggcat ctgaatagcg ccttgcccac
    63961 gcagcacacg ctgcgtcagc cagattcgcc catgatcggg gtagacaaat gcactgttcc
    64021 gcatggctct gccaaaccat tcatcacccg gagagtcgtc ccagacgggt gccagaatca
    64081 gcaattcagg aggggaacgc tcgtcttttc cgtcacgctt taactgacca ttaatatcgc
    64141 gaatatgccg ctgtaatcgc cccgctcgct gaatcagcaa atcaacaggg gccaggtcgg
    64201 agatcatttc gtccaggtca caatcaacgc tctgctctaa gacctgagta caaatgagga
    64261 cttttccggc acgctgtgaa ccgtcttctt taccaaagcg tgccagcgtc tccatttcaa
    64321 ttcgctggcg atcgctaaaa gcaaagcggc tatgaaagag tgaaaggctg gaagcgggaa
    64381 tgacgccgcg ggcaagtaac tgacgatgaa ctttaatagc gtcatcgaca gaattccgga
    64441 tccaggcgat gcatttcccc tgacttaccg aagattcgat acgcgcaata cattcttgtt
    64501 cgctatggag ccaacccacg ctcacgctac gctcaacgtc tttgcgcgtc gctacccggt
    64561 gcgaattcac atccgtttgc gtgacatgcg tcagccaggg gtaatcatct ttttccagca
    64621 atggagcttc ttgctggccc tctgtgccac gcgcaaaggc ggtaacaagt ttgtcgcgct
    64681 gctgttgaga taacgtggca gaaagcaaaa taacgctgtt tccaccacgc gcctgtcgct
    64741 cgatcagccc ttcaagaatg caagacatgt aagcgtcaca ggcgtgaatc tcatcggcca
    64801 gcaggatttt gttactcagc cccagaagcc gcagattatt atgtttaaac ggcatcaccg
    64861 ccatcatcgc ctgatccagg gtgccgacgc caatttcagc cagcaacgcc ttcttgttac
    64921 tgtcggcaaa ccaggccgca caaccctggc tgaaagcttg ttcatccggt tcttctgacc
    64981 cgattaaatc accggaccag agtgattcat taaagcggtc cattaatgtg cgggcactgt
    65041 gtgccagcac cagactgggg cgagattctg gcgaatagaa agaaagccag gttttgacga
    65101 gccgatcata catggtattg gccgttgcca ttgttggcag gccaaaaaac aaaccctgag
    65161 ctttacccgc agccatcaac ctgtgcgcaa gaataagcgc cgcttctgtt ttacctgcac
    65221 cagtcacatc ttccagaata aataactgtg gcccaggctg gctgatatcc agatccagca
    65281 ccttttgctg taacggtgtc ggattctcaa taaaaggaaa cagcgtatta attccggtga
    65341 aaggcgcgac ttttgctttt agaggaaaga cggttaaggc gttttgagcc tgaactaaag
    65401 ttttctgcca gtaaccttta atatccattg ggtgtgcgac gcgtggaaaa aatcgcgttg
    65461 acgaacctgt ccagtctgcg agtacgacgg ttgcagagat ataccaggaa agttgtttta
    65521 taagttcaat gccctcgtca tcatcccaga atgcggggat ctctattcgc ggaaacagtg
    65581 ccttgattgc aaggagaaaa tctcgtgctg cagctttgtc ttcaggcaga aaattatcca
    65641 attcatccat acgatcaggg ggccgaccat gatgccctgt cgtcacgggc atccacattt
    65701 ctatcactcg cctaagtttg cgcggagaga gcgaagatga aggaagtaac tcctgacatt
    65761 cacttaaata atgattccac aaccagtagc ccagcgttga atgagagatt ttttcgtaat
    65821 tttttctggc accttcctga atcttgagtt cagggggcag gtaaagttgt tgaaaagagt
    65881 gggcaaattt tccaatatcg tgccagcaca gcgaccaggc gaaaaattga gccgcctgtt
    65941 ccttgtcaga aatccctaat tgataaaaat aatcagccag cccgaagcaa tttcttttaa
    66001 ccattaaata gcccatcgcg gccacatcca gcgaatgcca gcaaagaagg tgatagtcgt
    66061 cgcccccttc tttctcgccg cgtcgggttt ttccccagaa atcaaagaaa gtca
//
LOCUS       DAZGCQ010000013        65425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000013 DAZGCQ010000000
VERSION     DAZGCQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.013"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..752
                     /gene="comR"
                     /locus_tag="ACHK07_000924"
     CDS             120..752
                     /gene="comR"
                     /locus_tag="ACHK07_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO8101296.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(814..1353)
                     /gene="ycfJ"
                     /locus_tag="ACHK07_000925"
     CDS             complement(814..1353)
                     /gene="ycfJ"
                     /locus_tag="ACHK07_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8101297.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1580..2884)
                     /gene="ndh"
                     /locus_tag="ACHK07_000926"
     CDS             complement(1580..2884)
                     /gene="ndh"
                     /locus_tag="ACHK07_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO8101298.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3292..3834)
                     /gene="ycfP"
                     /locus_tag="ACHK07_000927"
     CDS             complement(3292..3834)
                     /gene="ycfP"
                     /locus_tag="ACHK07_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO8101299.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3857..4882)
                     /gene="nagZ"
                     /locus_tag="ACHK07_000928"
     CDS             complement(3857..4882)
                     /gene="nagZ"
                     /locus_tag="ACHK07_000928"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO8101300.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4893..5717)
                     /gene="thiK"
                     /locus_tag="ACHK07_000929"
     CDS             complement(4893..5717)
                     /gene="thiK"
                     /locus_tag="ACHK07_000929"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO8101301.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLHDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENAEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5698..6339)
                     /gene="lpoB"
                     /locus_tag="ACHK07_000930"
     CDS             complement(5698..6339)
                     /gene="lpoB"
                     /locus_tag="ACHK07_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO8101302.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6353..6730)
                     /gene="ycfL"
                     /locus_tag="ACHK07_000931"
     CDS             complement(6353..6730)
                     /gene="ycfL"
                     /locus_tag="ACHK07_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO8101303.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6733..7092)
                     /gene="hinT"
                     /locus_tag="ACHK07_000932"
     CDS             complement(6733..7092)
                     /gene="hinT"
                     /locus_tag="ACHK07_000932"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO8101304.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7426..9615
                     /gene="fhuE"
                     /locus_tag="ACHK07_000933"
     CDS             7426..9615
                     /gene="fhuE"
                     /locus_tag="ACHK07_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO8101305.1"
                     /translation="MLSTQFNRDNQHQAITKPSLIAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQTTLNATHSEVE
                     FDSKMMYVDAYINKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGRYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(9675..11108)
                     /gene="ptsG"
                     /locus_tag="ACHK07_000934"
     CDS             complement(9675..11108)
                     /gene="ptsG"
                     /locus_tag="ACHK07_000934"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO8101306.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11403..12200)
                     /gene="ycfH"
                     /locus_tag="ACHK07_000935"
     CDS             complement(11403..12200)
                     /gene="ycfH"
                     /locus_tag="ACHK07_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8101307.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12211..13215)
                     /gene="holB"
                     /locus_tag="ACHK07_000936"
     CDS             complement(12211..13215)
                     /gene="holB"
                     /locus_tag="ACHK07_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO8101308.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYNTLAPEKGKNTLGIDAVREITEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13212..13853)
                     /gene="tmk"
                     /locus_tag="ACHK07_000937"
     CDS             complement(13212..13853)
                     /gene="tmk"
                     /locus_tag="ACHK07_000937"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8101309.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRSRYLELAAQDNSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13843..14865)
                     /gene="yceG"
                     /locus_tag="ACHK07_000938"
     CDS             complement(13843..14865)
                     /gene="yceG"
                     /locus_tag="ACHK07_000938"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO8101310.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14868..15677)
                     /gene="pabC"
                     /locus_tag="ACHK07_000939"
     CDS             complement(14868..15677)
                     /gene="pabC"
                     /locus_tag="ACHK07_000939"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO8101311.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15797..17038)
                     /gene="fabF"
                     /locus_tag="ACHK07_000940"
     CDS             complement(15797..17038)
                     /gene="fabF"
                     /locus_tag="ACHK07_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO8101312.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17126..17362)
                     /gene="acpP"
                     /locus_tag="ACHK07_000941"
     CDS             complement(17126..17362)
                     /gene="acpP"
                     /locus_tag="ACHK07_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8101313.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17573..18307)
                     /gene="fabG"
                     /locus_tag="ACHK07_000942"
     CDS             complement(17573..18307)
                     /gene="fabG"
                     /locus_tag="ACHK07_000942"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO8101314.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18320..19249)
                     /gene="fabD"
                     /locus_tag="ACHK07_000943"
     CDS             complement(18320..19249)
                     /gene="fabD"
                     /locus_tag="ACHK07_000943"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO8101315.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WAMTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19265..20218)
                     /gene="fabH"
                     /locus_tag="ACHK07_000944"
     CDS             complement(19265..20218)
                     /gene="fabH"
                     /locus_tag="ACHK07_000944"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO8101316.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20286..21356)
                     /gene="plsX"
                     /locus_tag="ACHK07_000945"
     CDS             complement(20286..21356)
                     /gene="plsX"
                     /locus_tag="ACHK07_000945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO8101317.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21437..21610)
                     /gene="rpmF"
                     /locus_tag="ACHK07_000946"
     CDS             complement(21437..21610)
                     /gene="rpmF"
                     /locus_tag="ACHK07_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO8101318.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21662..22183)
                     /gene="yceD"
                     /locus_tag="ACHK07_000947"
     CDS             complement(21662..22183)
                     /gene="yceD"
                     /locus_tag="ACHK07_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO8101319.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22382..22966
                     /gene="yceF"
                     /locus_tag="ACHK07_000948"
     CDS             22382..22966
                     /gene="yceF"
                     /locus_tag="ACHK07_000948"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO8101320.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23078..24037)
                     /gene="rluC"
                     /locus_tag="ACHK07_000949"
     CDS             complement(23078..24037)
                     /gene="rluC"
                     /locus_tag="ACHK07_000949"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO8101321.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24610..27795
                     /gene="rne"
                     /locus_tag="ACHK07_000950"
     CDS             24610..27795
                     /gene="rne"
                     /locus_tag="ACHK07_000950"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO8101322.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27990..28943)
                     /gene="flgL"
                     /locus_tag="ACHK07_000951"
     CDS             complement(27990..28943)
                     /gene="flgL"
                     /locus_tag="ACHK07_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO8101323.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPGGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28955..30598)
                     /gene="flgK"
                     /locus_tag="ACHK07_000952"
     CDS             complement(28955..30598)
                     /gene="flgK"
                     /locus_tag="ACHK07_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO8101324.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30664..31605)
                     /gene="flgJ"
                     /locus_tag="ACHK07_000953"
     CDS             complement(30664..31605)
                     /gene="flgJ"
                     /locus_tag="ACHK07_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO8101325.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31605..32702)
                     /gene="flgI"
                     /locus_tag="ACHK07_000954"
     CDS             complement(31605..32702)
                     /gene="flgI"
                     /locus_tag="ACHK07_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO8101326.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32714..33412)
                     /gene="flgH"
                     /locus_tag="ACHK07_000955"
     CDS             complement(32714..33412)
                     /gene="flgH"
                     /locus_tag="ACHK07_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO8101327.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33465..34247)
                     /gene="flgG"
                     /locus_tag="ACHK07_000956"
     CDS             complement(33465..34247)
                     /gene="flgG"
                     /locus_tag="ACHK07_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO8101328.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34419..35174)
                     /gene="flgF"
                     /locus_tag="ACHK07_000957"
     CDS             complement(34419..35174)
                     /gene="flgF"
                     /locus_tag="ACHK07_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO8101329.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGLVLQADPTLRVMSGVLEGSNVNTVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35194..36402)
                     /gene="flgE"
                     /locus_tag="ACHK07_000958"
     CDS             complement(35194..36402)
                     /gene="flgE"
                     /locus_tag="ACHK07_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO8101330.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLKFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(36427..37122)
                     /gene="flgD"
                     /locus_tag="ACHK07_000959"
     CDS             complement(36427..37122)
                     /gene="flgD"
                     /locus_tag="ACHK07_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO8101331.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37134..37538)
                     /gene="flgC"
                     /locus_tag="ACHK07_000960"
     CDS             complement(37134..37538)
                     /gene="flgC"
                     /locus_tag="ACHK07_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO8101332.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37542..37958)
                     /gene="flgB"
                     /locus_tag="ACHK07_000961"
     CDS             complement(37542..37958)
                     /gene="flgB"
                     /locus_tag="ACHK07_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO8101333.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTTPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38113..38772
                     /gene="flgA"
                     /locus_tag="ACHK07_000962"
     CDS             38113..38772
                     /gene="flgA"
                     /locus_tag="ACHK07_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO8101334.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNASVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38848..39141
                     /gene="flgM"
                     /locus_tag="ACHK07_000963"
     CDS             38848..39141
                     /gene="flgM"
                     /locus_tag="ACHK07_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO8101335.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQDLQSN"
     gene            39146..39562
                     /gene="flgN"
                     /locus_tag="ACHK07_000964"
     CDS             39146..39562
                     /gene="flgN"
                     /locus_tag="ACHK07_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO8101336.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39602..41137)
                     /gene="murJ"
                     /locus_tag="ACHK07_000965"
     CDS             complement(39602..41137)
                     /gene="murJ"
                     /locus_tag="ACHK07_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO8101337.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41247..42170)
                     /gene="yceM"
                     /locus_tag="ACHK07_000966"
     CDS             complement(41247..42170)
                     /gene="yceM"
                     /locus_tag="ACHK07_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8101338.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSAGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42172..42819)
                     /gene="yceH"
                     /locus_tag="ACHK07_000967"
     CDS             complement(42172..42819)
                     /gene="yceH"
                     /locus_tag="ACHK07_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO8101339.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42830..43414)
                     /gene="rimJ"
                     /locus_tag="ACHK07_000968"
     CDS             complement(42830..43414)
                     /gene="rimJ"
                     /locus_tag="ACHK07_000968"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8101340.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43650..44858
                     /gene="mdtH"
                     /locus_tag="ACHK07_000969"
     CDS             43650..44858
                     /gene="mdtH"
                     /locus_tag="ACHK07_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO8101341.1"
                     /translation="MSRVSQARSLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44922..45569
                     /gene="grxB"
                     /locus_tag="ACHK07_000970"
     CDS             44922..45569
                     /gene="grxB"
                     /locus_tag="ACHK07_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO8101342.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45703..46263
                     /gene="yceB"
                     /locus_tag="ACHK07_000971"
     CDS             45703..46263
                     /gene="yceB"
                     /locus_tag="ACHK07_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8101343.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46369..47415
                     /gene="pyrC"
                     /locus_tag="ACHK07_000972"
     CDS             46369..47415
                     /gene="pyrC"
                     /locus_tag="ACHK07_000972"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO8101344.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47489..47734
                     /gene="dinI"
                     /locus_tag="ACHK07_000973"
     CDS             47489..47734
                     /gene="dinI"
                     /locus_tag="ACHK07_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO8101345.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48024..48278
                     /gene="bssS"
                     /locus_tag="ACHK07_000974"
     CDS             48024..48278
                     /gene="bssS"
                     /locus_tag="ACHK07_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO8101346.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48393..49511
                     /gene="solA"
                     /locus_tag="ACHK07_000975"
     CDS             48393..49511
                     /gene="solA"
                     /locus_tag="ACHK07_000975"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO8101347.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49559..49672
                     /locus_tag="ACHK07_000976"
     CDS             49559..49672
                     /locus_tag="ACHK07_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO8101348.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49933..50499
                     /gene="yceJ"
                     /locus_tag="ACHK07_000977"
     CDS             49933..50499
                     /gene="yceJ"
                     /locus_tag="ACHK07_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8101349.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50503..51078
                     /gene="yceI"
                     /locus_tag="ACHK07_000978"
     CDS             50503..51078
                     /gene="yceI"
                     /locus_tag="ACHK07_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO8101350.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51120..52172)
                     /gene="trhO"
                     /locus_tag="ACHK07_000979"
     CDS             complement(51120..52172)
                     /gene="trhO"
                     /locus_tag="ACHK07_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO8101351.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52397..53317
                     /gene="lpxL"
                     /locus_tag="ACHK07_000980"
     CDS             52397..53317
                     /gene="lpxL"
                     /locus_tag="ACHK07_000980"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO8101352.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53489..54715
                     /gene="mdtG"
                     /locus_tag="ACHK07_000981"
     CDS             53489..54715
                     /gene="mdtG"
                     /locus_tag="ACHK07_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO8101353.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            54798..55172
                     /gene="msyB"
                     /locus_tag="ACHK07_000982"
     CDS             54798..55172
                     /gene="msyB"
                     /locus_tag="ACHK07_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8101354.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55173..55400)
                     /gene="yceK"
                     /locus_tag="ACHK07_000983"
     CDS             complement(55173..55400)
                     /gene="yceK"
                     /locus_tag="ACHK07_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8101355.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55573..58116)
                     /gene="mdoH"
                     /locus_tag="ACHK07_000984"
     CDS             complement(55573..58116)
                     /gene="mdoH"
                     /locus_tag="ACHK07_000984"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO8101356.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPLDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(58109..59644)
                     /gene="mdoG"
                     /locus_tag="ACHK07_000985"
     CDS             complement(58109..59644)
                     /gene="mdoG"
                     /locus_tag="ACHK07_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO8101357.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPSNSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            60037..61194
                     /gene="mdoC"
                     /locus_tag="ACHK07_000986"
     CDS             60037..61194
                     /gene="mdoC"
                     /locus_tag="ACHK07_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO8101358.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSLIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61202..62683)
                     /gene="clsC"
                     /locus_tag="ACHK07_000987"
     CDS             complement(61202..62683)
                     /gene="clsC"
                     /locus_tag="ACHK07_000987"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO8101359.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62625..63158)
                     /gene="ymdB"
                     /locus_tag="ACHK07_000988"
     CDS             complement(62625..63158)
                     /gene="ymdB"
                     /locus_tag="ACHK07_000988"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO8101360.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENSHLYERLLTQQGDE"
     gene            complement(63253..63564)
                     /gene="ymdA"
                     /locus_tag="ACHK07_000989"
     CDS             complement(63253..63564)
                     /gene="ymdA"
                     /locus_tag="ACHK07_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO8101361.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNSQVASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63685..64017)
                     /gene="csgC"
                     /locus_tag="ACHK07_000990"
     CDS             complement(63685..64017)
                     /gene="csgC"
                     /locus_tag="ACHK07_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO8101362.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(64076..64531)
                     /gene="csgA"
                     /locus_tag="ACHK07_000991"
     CDS             complement(64076..64531)
                     /gene="csgA"
                     /locus_tag="ACHK07_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO8101363.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(64572..65027)
                     /gene="csgB"
                     /locus_tag="ACHK07_000992"
     CDS             complement(64572..65027)
                     /gene="csgB"
                     /locus_tag="ACHK07_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO8101364.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(65227..>65425)
                     /locus_tag="ACHK07_000993"
     CDS             complement(65227..>65425)
                     /locus_tag="ACHK07_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101365.1"
                     /translation="SFRHVKSCCFFSTPPWTIFYCKKTRFVTACAQDISQQSATGKKN
                     DCLVIFDSGNGDLKENKIFFA"
BASE COUNT        15972 a        17394 c        16045 g        16014 t
ORIGIN      
        1 aaaatcttta aatttcagtt aaatagaaag caattatttt tatgggatct ggactggtga
       61 acggaggtga ttatttttat aataaccttt cattcacagt caccaggtac aacggcatca
      121 tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca
      181 gggatgccgc gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt
      241 ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta
      301 ccaacaaaga ggggttattt cgcgccgtac tcgaccgcta tatcgatcgt tttgccgcta
      361 agcatgaagc acagctgttt tgtgaagaga aaagcgtgga gtcagcgctg gctgactatt
      421 ttgctgccat cgccaactgc tttaccagca aagacacccc ggctggctgc ttcatgatca
      481 acaactgcac caccctctcc ccagattcag gagatatcgc caatacgctg aaatcacgcc
      541 atgcgatgca agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa
      601 tcccgaccca ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga
      661 tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact gatgcagatt gcccgaacga
      721 ctttgcgttt atggccggaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc
      781 gaatgagggg ctaaattgca gagtacaacc ttgttatact ttgttattca aaatcagttg
      841 cccgttgcta tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg
      901 ctgatcgcca atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac
      961 cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat
     1021 ctggttaccg gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccacg
     1081 accaccacca aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt
     1141 aatgcgattt tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg
     1201 acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg
     1261 gccacgttca aacacgttca gactggccac tgccgctacg cccagcgcag ctgcgacacc
     1321 aatcccgata cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg
     1381 ccaatcatgc aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgtgggaat
     1441 ggctggaaat acttaaggat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg
     1501 cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac
     1561 atccgccagt gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac
     1621 tccccaccag catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc
     1681 tgtatagcga gatgtacaca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg
     1741 tcaggttacc catcaggcta ccgacggtgg agaagttcga cagcgatacc agcgaaccgt
     1801 gatctttata ctgataattt ttcagcggct taccgttcat ctgcgccaga atgttgttca
     1861 ttgcgcaagt tgccatctgg tgtgcagcct gagcgcgcgg cggaacaaag cccccttccg
     1921 gacgcgggca tgacgcgcaa tcgccaatag cgtaaatgtc tggatcgcgg gtggtttgca
     1981 gcgtcggttc caccaccagc tggttgatac ggttggtttc aagaccacca atatctttca
     2041 ggaagtctgg cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcgc
     2101 catctttagt gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc
     2161 gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga gatacgtggc ggtaatgcag
     2221 gcaaaatacg ttcacccgct tctaccagcg ttacgttcag ggcttcgttg gtcaggcctt
     2281 tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcag ttcagcggag agttctacac
     2341 ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc atttgcgccc aggttggcgg
     2401 agtatttcag gaagagattc agcatctcct ggtgaaagcg acgcgcctgg tgcgggttat
     2461 cgaggaaaat gcagttctct ttgacacctg gcgtattgaa atcgttagag gtgctaccca
     2521 gcgccattac cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct
     2581 catcgcgcag ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac
     2641 ccagctggaa ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc
     2701 cttcatcaag cgagccagtc gccacttcgt gcagcagcgg tttccacagg tggctgtggt
     2761 tacgatcgac cagcgtaatt ttggcttttt tcttgcgtcc cagcttatgc cccagctgtg
     2821 ttgccatttc cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag
     2881 tcaacgtgac ccccttaaat ttattaacca attgttaatt aatggttatt aacatagctc
     2941 ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg
     3001 gtgcattggt cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac
     3061 tttaccgggt gagatcaaaa aacagagttt attgtttatt atttgagcga atctggaagg
     3121 gtgatatagg gtgaagcgta tatcgagggc gtttggtcgt actgataaga tgcgcagcat
     3181 cgcatcaggc attgatgctc gactgtcgga tgcggcgtga acgccttatc cgacttacag
     3241 aatccggagc gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag
     3301 ggttttgaac gctttaatgc gctgcaaatg cggagagata ttcttgaatt tgtgcgtctg
     3361 ctcttcgtcc cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt
     3421 aagcgcttca tcattacgcg acaaaatcac cagacaacga tcgcgattct tctcgcggaa
     3481 gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc
     3541 catgttctca taagggaaca aattagggtt gaagatcacc tggcggatgt cgcaaagaaa
     3601 accaatccgt tccgcccagt atccgcccaa gccaacgccg caaattagcg gacgctcgtc
     3661 aacgttcagt tgcaacattt tgtccacttc tttaagcaga tgctgcatat catgtttcgg
     3721 atgccgcgta ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac
     3781 tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc
     3841 ctcacataag ccagggttag tgacctgctt tctcttcctg ccagcgttca tgtaactgat
     3901 tcagacgggc gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg
     3961 aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca
     4021 cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaaccc gcatccagtg
     4081 atgcctgccc gcgttcggca taactgccca taatcgcggc accttccatc gataaatcgt
     4141 cagagaaaat cacgccgtca aaacccagtt cctgacgcaa aacggttttc agccagtagg
     4201 gagaaccgct cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg
     4261 cgtcgagttt gttttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct
     4321 ccgcttgtgg acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt
     4381 gtccagggaa gtgtttcccg gtcgttttca ttccggcttc atgcatacca tcaataaacc
     4441 ggctggcaat tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg
     4501 cgctgatatg cccgacatcc agcacaggcg caaagctgat atcaatatcc atagcgatca
     4561 tttcgctggc catcaaccaa cctgcctcct gcgccagttt gccaccctct tccattcctg
     4621 acagcgcagc gaatgattgc gccgctggca agcgggtaaa accttcacga aaacgctgca
     4681 cgcgtccacc ttcctgatca accgccacca ccagatgatt gcgcgaagct gcgcggatct
     4741 ggcgcaccag ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc
     4801 ctcccaccag cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga
     4861 catccaacat tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag
     4921 gtgtcatcgg ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac
     4981 caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc
     5041 gccggataaa tcttcgcgcg agtggcatag tcattgacca attgtcggtg ctgttcagca
     5101 ttttccaccc acaccgccgc cagttccagc gcgatatcac catctcctgc atactcccag
     5161 tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc
     5221 aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taacattcgc
     5281 aaccaaccca ctgtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaacggc
     5341 aacaacgtta ttcgccagcc aaaacgtggt tgttgatgta gataatacag caagcctgcc
     5401 agttcgttgg tatccggcaa atacgttttt accgcgccgg gcagatagtc gactaccatc
     5461 cagtcatgga gatataaatg cggcttcggt gcaatgcatg cgggtagttg tgataaagcc
     5521 cgatactggc gcaagaacgc tgactgcggc gcatcaggat cgtgcggctg acgcacaaca
     5581 aaacgctggc cctggtgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc
     5641 gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta
     5701 ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag
     5761 ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg
     5821 agcgccgaca ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg
     5881 cgacaaacct aactgttgct tcgccatcga cagctgctgg gcggaaacca gggtaaattt
     5941 cccgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc
     6001 gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca ccccgtcagc
     6061 cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg
     6121 cggcgcaggc ggtgccgttt gatcttcgtg ctcaattggg cctggctgct gcgggatcgt
     6181 cggcaccgag ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg
     6241 tttcacttct tctaccggtg caggttcacg ttgccccaca cacccggcga gaaacatcgc
     6301 cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat
     6361 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
     6421 ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc
     6481 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
     6541 gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
     6601 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattt accggaattt
     6661 ccggatgtga acgacagccc accagcagta acaacgccag agacaccagc ccaaagcatc
     6721 cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa
     6781 caagtgcata tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag
     6841 acgataacca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
     6901 acgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt
     6961 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
     7021 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
     7081 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
     7141 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg
     7201 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
     7261 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gtcaaatatg aatgcgtata
     7321 tttctcattt gcatttacat acaaaattat tcgcacacta aatataaccg ctcatcaccc
     7381 tgctccggca tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa
     7441 tttaacaggg ataatcaaca tcaagccatc accaaaccgt cactaattgc cggttgcata
     7501 gcactggcac tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt
     7561 gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc
     7621 tctgcgggta ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt
     7681 agccagcagc ggatggaaga tcagcagcta caaacgctgg gcgaagtgat ggaaaacacg
     7741 ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc
     7801 cagatcgata actatatggt tgatggtatc cctacctatt ttgaatcgcg ctggaatctg
     7861 ggcgacgcac tttctgatat ggcacttttt gaacgcgtag aagtagtgcg tggcgcgaca
     7921 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg
     7981 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg
     8041 tatgtggcgg atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc
     8101 ggcggctacc agaataacga ctcatggctg gaccgctaca atagtgaaaa gaccttcttc
     8161 tccggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat
     8221 cagcgcattg atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc
     8281 agcagcaaca gttacgatcg tgcacgcagt accgcacctg actgggcgta caacgataaa
     8341 gagatcaaca aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaaacgacg
     8401 ctgaatgcca cccactccga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat
     8461 ataaacaaag cggatggtat gctggttggg ccatacagta attatggacc tggttttgat
     8521 tatgtcggcg gcaccggttg gaacagcggc aaacgtaaag ttgatgcgct ggatttgttc
     8581 gctgacggtc gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac
     8641 agcaaacaaa acaatcgtta cttcagttca tgggccaata ttttcccgga tgaaattggc
     8701 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg
     8761 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat
     8821 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac
     8881 agcatggaga aaaaccacac cacgccttat gctggtctag tgtttgacat caatgacaac
     8941 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
     9001 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
     9061 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct
     9121 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
     9181 gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag
     9241 ctgacatttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct
     9301 aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag
     9361 ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg
     9421 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
     9481 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac
     9541 gataccaacg tggaaggttc tatcgtctac ggcacaccgc gtaatttcag cattaccggc
     9601 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc
     9661 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
     9721 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
     9781 agtttcttca ggccggcctg atccacttta gacacatcag caacgctgac gcgcagacgg
     9841 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
     9901 ggtgccattt cgctggtacc tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc
     9961 gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa
    10021 ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag
    10081 tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg
    10141 aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag
    10201 gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata
    10261 ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca
    10321 gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca
    10381 cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca
    10441 ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa
    10501 cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga
    10561 gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta
    10621 aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga
    10681 tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag
    10741 ataatccctc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt
    10801 aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca
    10861 actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca
    10921 aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca
    10981 acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc
    11041 agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc
    11101 ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc
    11161 aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag
    11221 taaaataatt cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga
    11281 gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc
    11341 caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc
    11401 attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg
    11461 gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca
    11521 acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa
    11581 ggtgagtcag tttccaccag taaccgatcc agggggacat aacgcgcagc atcgcgcagt
    11641 tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt
    11701 aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc
    11761 actttttctt cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata
    11821 accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt
    11881 actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc
    11941 tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga
    12001 tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa
    12061 taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc
    12121 gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag
    12181 tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc
    12241 cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat
    12301 gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag
    12361 gcgcgaggga gaaagatggt ttgccagttc gacgaccagg cccggcacat caacattggt
    12421 cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg
    12481 taaacgcgcc ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga
    12541 tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg
    12601 aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaaa cgcaatgcgg caagtaatgc
    12661 atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg
    12721 cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg
    12781 ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt
    12841 agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctaa
    12901 gcgtgcgtgc tcattcagct tttcggtgat ctcacgtacc gcatcaatgc ccagcgtatt
    12961 tttccctttt tcgggagcca gggtgttgta atcgggatgc gtgccagcct gcatcaactg
    13021 acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg
    13081 gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc
    13141 atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca
    13201 tggataccat ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca
    13261 tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactgtt atcttgtgca
    13321 gccagttcca gatagcggga gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata
    13381 cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga
    13441 tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata
    13501 tgttggtcaa taccacgtcc gccgccctga tacgcctgag tggagagatc gtggcgatcg
    13561 ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg
    13621 cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat
    13681 ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccgc
    13741 gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct caaccaccac attacgcgcg
    13801 gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc
    13861 cttaagcact ttcagataat cctgcacaga cttgttatga cttgcaagat tggtattaaa
    13921 cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc
    13981 agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat
    14041 ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata
    14101 acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa
    14161 acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc
    14221 gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg
    14281 cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc
    14341 gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca
    14401 ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt
    14461 gtgcttgata tacggagcct cgcgcaattg cttgaggtag tcgctcagac gcatcccttc
    14521 taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg
    14581 cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc
    14641 gatacgcagc aaccattgaa aaacccgtgg gcgattgatg atcttatcgg cataaagctg
    14701 ttcaccaagc gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat
    14761 aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc
    14821 cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc
    14881 tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag
    14941 gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac
    15001 gcctcttcca gagaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg
    15061 atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact
    15121 acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg
    15181 tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc
    15241 tcaagacgat tgagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg
    15301 cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt aatgtgcagg ataagccgta
    15361 acggagagaa tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg cccgccacta
    15421 ccgcgactga tcacgacttt cagtacacca ttttgctgtt ctgctgccag cgttttcatc
    15481 tcttgttcaa gctgtggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt
    15541 agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta
    15601 aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa
    15661 ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca
    15721 ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg
    15781 ggtggaaaat gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc
    15841 cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga
    15901 aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat
    15961 cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac
    16021 cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt
    16081 tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt
    16141 agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg
    16201 cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc
    16261 cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta
    16321 ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc
    16381 tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa
    16441 cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat
    16501 acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga
    16561 tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca
    16621 caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg
    16681 tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc
    16741 gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga
    16801 caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct
    16861 cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat
    16921 ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg
    16981 tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt
    17041 ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt
    17101 cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag
    17161 cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt
    17221 ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag
    17281 aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa
    17341 cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg
    17401 ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg
    17461 attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt
    17521 actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca
    17581 tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg
    17641 ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct
    17701 gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa
    17761 agcccggagc aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt
    17821 tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca
    17881 tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc
    17941 gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc
    18001 actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca
    18061 ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg
    18121 ggtcggtcac attcaacatc agacctttgc cgttagcacc taaataatca ctgatcgcct
    18181 gagcgccatt ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt
    18241 cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa
    18301 aattcataat tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca
    18361 gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc actttgcccg
    18421 ggccgacttc atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc
    18481 actgaaccgg gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt
    18541 cgcatttcac atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg
    18601 ctaattctac tgccagtttg tcggctgctg gtttcatcag cgcacagtga gatggtacgc
    18661 tcactggtaa cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct
    18721 caaccgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag
    18781 aaacgacctg accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac
    18841 cgatgattgc cgccatagcg cccgttcctt ccggtacggc ttcttgcatg aacttgccgc
    18901 gcatctcaac cagacgcacc gcatcagcga aatcaatcac accagcgcaa accagcgcgg
    18961 agtattcccc caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc
    19021 atacgcggta cagcgcaaca gaggcagtca acagcgctgg ctgggtttgc caggttttat
    19081 tcagttcttc agctggcccc tgctgggtca tcgcccacag gtcgtagccc agcgccgcag
    19141 aagcttctgc aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc
    19201 caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct
    19261 tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca
    19321 gaaccaactg ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga
    19381 cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca
    19441 taccgagttt tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc
    19501 agtccagttg agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca
    19561 gttccgttac cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct
    19621 ctggattcac gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat
    19681 gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc
    19741 catcgccaaa aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat
    19801 cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc
    19861 ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa
    19921 tgcccaacat gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg
    19981 ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg
    20041 tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc
    20101 ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg
    20161 cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt
    20221 acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg
    20281 tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat
    20341 acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc
    20401 tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg
    20461 ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc
    20521 ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc
    20581 ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt
    20641 aatgtgacat ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac
    20701 tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag
    20761 gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag caacgccacg
    20821 cgaggattgg gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc
    20881 accaacattg tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc
    20941 ttttgctgat gcggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg
    21001 agtaataatt ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc
    21061 gctcgacctt ctttcaccag ctccagggcc acgcgcattg aactcccacg actggcgcgg
    21121 atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga
    21181 cgcgaacgtt gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg
    21241 actaaaagaa gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc
    21301 acggaagggc caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta
    21361 tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca
    21421 tcacgcagac gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag
    21481 tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg
    21541 cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt
    21601 gtacggccat ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca
    21661 attacttacg ctttaagctg gctaatacgg caaatgggtt tggcttttgc gcttcttcag
    21721 gcagttcacc aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg
    21781 gaactaccgg caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac
    21841 cgaattcgtt aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg
    21901 aacgcacagg actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac
    21961 gctgacactc gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt
    22021 tatcgatagc gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg
    22081 cgacgcgctc aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg
    22141 tacgaaccgg atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac
    22201 tttgtaacgt catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc
    22261 gcacacattg cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc
    22321 aaaacgaagc gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa
    22381 tatgcctaaa cttattttag cctccacctc gccctggcgt cgcgctcttc tggaaaaact
    22441 gcaaatctct tttgaatgtg cagcacctga ggtcgacgaa accccacgca gcgacgaatc
    22501 accgcgacag ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcacgtta
    22561 tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg
    22621 taaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt
    22681 caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctgc aaacagaagt
    22741 ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg
    22801 taaggagcat cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct
    22861 gtttgagcgt ttagaggggc gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct
    22921 gtgtcagatg ttacgtcggg aagggaaaaa tccgctgatg ggatagtttt ttgcgccaga
    22981 tgcggttacg tcgcatccgg caatcggtgc tggcggcctg atgcggcgta aacgccttat
    23041 caggccaacc gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt
    23101 gcaaacaacg cttcaaacca tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc
    23161 ccggatgggt aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg
    23221 cttcagtgag ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat
    23281 gacccgcata ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacagc
    23341 gcaccagggt ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc
    23401 cttcctgact cacacgcacg atacgttcgc cgctttgcag aatatttttc agtaacggcg
    23461 cttgaacgct cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt
    23521 gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta
    23581 ccagcaatac acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt
    23641 ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta
    23701 ccgccgtacc ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt
    23761 ccgccagcgc cgccaccttt tgcagatgtg gtgaaaccgc ctcttcttcc cgctcagcaa
    23821 cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac
    23881 gttttttgtt cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta
    23941 cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga
    24001 tagcaacaat ttttacggat ggagtctctg ttttcatggt tggcgattct aattagccaa
    24061 caggattcgc gccactcatt tttctatgct tatatttact tttgcacctt attacttcac
    24121 tgcgtgctca ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt
    24181 gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata
    24241 atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt
    24301 tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag
    24361 cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag
    24421 tctgtttctt tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc
    24481 gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg
    24541 ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag
    24601 taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc
    24661 cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa
    24721 aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt
    24781 gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat
    24841 ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt
    24901 caggaggtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc
    24961 acctttatca gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt
    25021 ggcatttctc gccgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc
    25081 cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct
    25141 gaggcgctgc aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc
    25201 gctgaaagcc gcccggcccc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca
    25261 ttccgcgatt acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc
    25321 gaactggcac gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa
    25381 ctgtacaccg gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc
    25441 gccttccagc gtgaagtccg tctgccatcc ggtggttcga ttgttatcga cagcaccgaa
    25501 gcgttaacag ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa
    25561 actgcgttta acaccaacct cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt
    25621 gacctaggcg gcctgattgt tatcgacttc atcgacatga cgcctgtacg ccaccagcgt
    25681 gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc
    25741 catatttctc gctttggcct gctggaaatg tcccgtcagc gcctgagccc atcactgggt
    25801 gaatccagtc atcacgtttg tccgcgttgt tctggtactg gtaccgtgcg tgacaacgaa
    25861 tcgctgtcgc tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag
    25921 gaagttcacg ccattgttcc tgtgccaatc gcttcttacc tgctgaatga aaaacgttct
    25981 gcggtaaatg ccattgaaac tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat
    26041 cagatggaaa ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc
    26101 ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag
    26161 ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg
    26221 ccgccagcgc caacgccagc tgaacctgcc gcgcctgttg tagccccagc accgaaagct
    26281 gcaccggcaa caccagcaac tcctgcacaa cctgggctgt tgagccgctt cttcggcgca
    26341 ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccga ccgagcaacc agcaccgaaa
    26401 gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt
    26461 gaccgtaatg agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa
    26521 gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt
    26581 cagcaggctg aggtaacgga aaaagcacgt accaccgacg agcaacaagc gccgcgtcgt
    26641 gaacgtagcc gccgccgtaa cgatgataaa cgtcagacgc aacaagaagc gaaggcgctg
    26701 aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag
    26761 ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa
    26821 gaagcggtag ttgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa
    26881 gcgccagctc cacgcacaga actggtgaaa gtcccgctac cagtcgtagc gcaaactgca
    26941 ccagaacagc aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt
    27001 tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac
    27061 gagcgttatc caacccaatc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg
    27121 gcctctggca aagtctggat ccgctatccg attgtacgtc cacaagatgt acaggttgaa
    27181 gagcagcgcg aacaggaaga agtgcaagtg cagccgatgg tgactgaggt gcctgtcgcc
    27241 gccgctgtcg aaccggttgt tagcgcgcca gttgttgaag aagtggctga agtcgtagaa
    27301 gcccccgttc agattgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagta
    27361 attgccgccg cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc
    27421 caggaagtcg cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt
    27481 gaagaagttg ccgaaactgc tgaggttgta gttgctgaac ctgaagttgt tgctcaacct
    27541 gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtaaccgc ggttaaacca
    27601 gagatcaccg ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa
    27661 tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt
    27721 aaaggtgccg caggtggtca tacggcaaca catcatgcct ctgccgctcc tgcgcgtccg
    27781 caacctgttg agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt
    27841 tatttcatct ttgaatcaac atattaaaat tcatcgaatg gcatccttgc taaccaacaa
    27901 tgcaaaatag gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac
    27961 ccagtttcat gatatgtttt aaagcgaaat tatttgttga gctggaagag cgacaatccc
    28021 tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat
    28081 gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa
    28141 gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc
    28201 gcgcgaacag tcagcacatt gttcagcgag tttttcagtc cgcggttggt tttatccagt
    28261 gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg
    28321 atggcactat ccagcatggc aaaaagattg gtttcagaag cgctaccgcc tggttccgct
    28381 accgcgttgc tggtaatact gtcgaaaatt ttgtcacccg tgtgccctat taccatcgaa
    28441 cgcgaagcat cgacctgttg tttaatactt tctgttccgc cgacataatc accgttagcc
    28501 tcactaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca
    28561 gtggtgttcg ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag
    28621 gcccggtcat cgtcactcaa agtgccattg ctggcgtaga caattttttc ctgagcattc
    28681 tggatagctg tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg
    28741 aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aactacggct
    28801 tgtgatgctg caatgggatc gtcagaaggg ttaacgactc gcttacctgt cgacatctgc
    28861 tcgccgtact tcatccattc cgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac
    28921 atcatctggg tactgaaacg catcttatct ccccttagcg aatgttaatc agcgcatcaa
    28981 aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt
    29041 gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa
    29101 gctgcgtcac cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac
    29161 cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt
    29221 tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat
    29281 cttcttcgct cgccatcgcg attttcgctt cgtcggtgat taatacatcc atgttgacga
    29341 tggcgtcact tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg
    29401 tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg
    29461 tattgctggc aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg
    29521 tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtta ccgttgtttt
    29581 tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcaccc tcatcgccgt
    29641 tggcatcaaa tccggctttg tgttgggtgt tgaaagcctc agcaaatgcc agcgccagtt
    29701 gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca
    29761 gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacat
    29821 aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac tgccgccaat tgccgcgccg
    29881 tacttccctg aaccagtgag taaccattgg ccatcgtgat gttataagtg ccgccatcct
    29941 gaacgctgac ttctacacca acaatctggt ttaattcgct caccagttga tcgcgttgat
    30001 ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgggag atttgatcgt
    30061 tcaggctggc gatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga
    30121 tattgacctg tttgtcctgg tcgcgaagat actgatcggt ggttttaaac tgattcacca
    30181 acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca
    30241 gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg
    30301 agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg
    30361 tctgcgccgc acgtaactgg ttggtaataa atgcatcata ctcacgctgc acaccagaaa
    30421 cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa
    30481 tagtagtttg gcgggtatat ccggcaacgt tatagctgga gatattatta cttgctgtat
    30541 ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg
    30601 aggttcctta taagccttca attacagagt attatcggca gcgccccgcc ggacttgagt
    30661 tattcagaac agattatcaa tgttcatact gtaggttttg ctcaccttgt cgcttatcga
    30721 tttcatctgc tgaatcatgt tggtgagttt acgggcatag tgaggatcgg tggcatagcc
    30781 cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca cggcggcgta
    30841 gcgcgggtta cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta
    30901 gacccgaaac ttcgctttta ctttcttcgc ttcgccattt tcatattcag tcgtggtgat
    30961 ttcagtgact ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg
    31021 ctcgccgttt tcgcggcgga tttgccgttg cccccagcca gattccagcg ccgcctgagc
    31081 gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag
    31141 ttgcgcgagg aatgctttac tgtcacctgg cagcgaatca tcgtagttac gtggcacggc
    31201 cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacggttt cgagcgggaa
    31261 tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgctt
    31321 aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg
    31381 gtcatacata ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa
    31441 agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac
    31501 cggacggata tttgccgccg ggtcttcgcc cgctttcgcc tttagttcgt tgagtgattg
    31561 cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt
    31621 tttgcccgca gacatcccgc actttgcatt gattgcagta tggacattaa atccattggg
    31681 gtggctccca aggcgttgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg
    31741 ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc
    31801 tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgtaact
    31861 gagagattcc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc
    31921 gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata
    31981 ttctggatat cggcaaggaa acggacctgg gagctgttgc cacttggtac gcgcacctga
    32041 atagtccgcg catccaacgc ggtggcgctg ccatatccac gcactcggtt gatggtgtca
    32101 gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg
    32161 acgccaaact ggctgggtaa ttcacgttca ataactgcac cattggtgat ccgtccaccg
    32221 ttcagttggt tcacctgaac actgctaccg ccagcggagg ctccagcgcc gccaaccaga
    32281 atattgccct gtgccagcgc atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac
    32341 aacgtacctc cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt
    32401 tgcccctgac gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt
    32461 agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc
    32521 gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc
    32581 agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata
    32641 cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc
    32701 accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat
    32761 tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag
    32821 tagacggtac ggtattgctg ccgctgatgg tgcgtggatt aaccacgcca gagaagcgaa
    32881 taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt
    32941 tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg
    33001 ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac
    33061 caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac
    33121 ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca
    33181 gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa
    33241 tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt
    33301 gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg
    33361 aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca
    33421 tcttcaaaaa tcaataaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt
    33481 ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc
    33541 acctgaatca tattgaccag ttcttccgcc acattgacgt tagacgtttc aacataccct
    33601 tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat
    33661 tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg
    33721 gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc
    33781 acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc
    33841 acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca
    33901 cgggtatagg ctgatgagcc atctggcaac atcacctgga aaaagccctg ccctttaatc
    33961 gcgacatctt tgctgttgtt ggtctgcgac aggttgccct ggctgtgtaa gcgttcagtg
    34021 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    34081 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    34141 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    34201 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    34261 gtggattagt gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac gccttatccg
    34321 gcctacaagt gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc gataaacttg
    34381 cgcatcgggc aacttatact tgtcatgtat cctttcaatt aactcatcga cagcagttgg
    34441 ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc
    34501 cgcgcgctgg caatcatgtc gctcattgcc gcaacggtat tgacattact gccttccaga
    34561 acacccgaca tcacacgcaa ggttggatct gcctgcagta caagcccacg cgtggcctgg
    34621 gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct
    34681 ttcaccagtt taagacgccc tactggcgca accgtatttg ccggatcgcc cggattcagc
    34741 gccgagattg tgccatcggc agcaatagtg atttccgccc cttccggcac agcaattggc
    34801 cccgcctcgc ctatcaccgg atgcccctga attgtcagtt gcccggtggg atcaacctga
    34861 atgctgccat tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc cagccagcca
    34921 tcctgctgca atgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc gggcgtcatg
    34981 tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc
    35041 ggcactgcgc gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga
    35101 ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcggtataa
    35161 attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca gcgtgttgag
    35221 gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct gggcaacgat
    35281 catattgacc agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag
    35341 ggtgccaaag tttcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc
    35401 agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac
    35461 aatctgcccc agcagttggg tttgttcatt ggaatagttg ccgacaaccg taccgtcatc
    35521 attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat
    35581 gttgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa acgtggcggg
    35641 ttctgcaccg ttaattgcgc cggttgcgat attattcgcc atcgcaccat ccactaatgt
    35701 gccattagca ttaaatttca gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct
    35761 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaca cgctcatgtc
    35821 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    35881 attgctggca tcaaatgcgt taacagaggg aagcggatcg ctggaattca ggttgatctg
    35941 catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt
    36001 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    36061 taaaccttgc atattcacca ggttacgatt ttcatccagc ttaaattgtc cgttacggct
    36121 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    36181 gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    36241 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    36301 tgatttaaag ccgtatgtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    36361 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    36421 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    36481 cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    36541 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    36601 cgcagtgctg ccatcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cggcggtcag
    36661 ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac
    36721 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc
    36781 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag
    36841 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt
    36901 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    36961 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa
    37021 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    37081 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    37141 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc
    37201 tgatagctgc gtgacgccga catggtgtta accatctctc cgacaacatc aacgttcggc
    37261 atttttacgt agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc
    37321 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    37381 gcgttaacct gaaataccac ctgttttgcc cgatatggct gtccatcggg accggtcacg
    37441 ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    37501 gccgacccgg cgatatcgaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    37561 acgttcatca tgcctttgat ttgcccgctc aacgcactaa ggctcatctg gtattgcagg
    37621 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    37681 ggctggtccg gaatacggta ttgcagttct gcggtaggag tcgtcagcgc ctgcgccgga
    37741 atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    37801 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    37861 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    37921 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    37981 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    38041 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    38101 ctgggggcga aaatgctggc aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    38161 ccgttaagtg cggcgagcaa tctcacatcg caattgcaaa ccttttttag ctcccagctc
    38221 gcagggataa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg
    38281 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    38341 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    38401 tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    38461 ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    38521 gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    38581 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    38641 tttagcgcca acgctgaagg tcaggcgctg aacaatgcat ccgtcgcaca gaatgcgcgg
    38701 gtgcgcatgg tatctggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    38761 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    38821 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc
    38881 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacac ccgggcggca
    38941 aaaacaacag catccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    39001 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    39061 aacggtgaac taaaaatgga catcggcaaa attgccgatg cgctgatcaa cgaagcgcag
    39121 caagacttgc agagtaatta atcgtatgac gcgtcttgca gagatcctcg accagatgtc
    39181 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    39241 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcaac
    39301 gttggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat
    39361 tagtcaacgc tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    39421 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    39481 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    39541 cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatgcatt
    39601 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    39661 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagcaagcgc cagggcatgg
    39721 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    39781 gtaccgccac caccagacgc aacagaaacg ccatccagcc aggttgcggg gtaaagattt
    39841 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    39901 aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc aattgcgtta
    39961 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    40021 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    40081 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    40141 tcaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa
    40201 gacgcagccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    40261 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    40321 ccattaagcg gtcggcgtaa tacatccacg acaccgaacc ggaagcaaga aacgaggcaa
    40381 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatt gccggtccca
    40441 tctgtttcac cacacgcatt gctccggcat cgtggaagtt aatgcgtggc aaaaccagca
    40501 tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggacgccg ccgaccgtaa
    40561 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    40621 caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg
    40681 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    40741 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    40801 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcga
    40861 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    40921 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    40981 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    41041 caaagattct ggcgacaatt gcgtcacgtg cgaagccaag cacacgcgaa aacatggtca
    41101 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    41161 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    41221 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    41281 acgatacgtt gcgccagcac ggcctgttcg ccggcggttt gcggaactgt ctgattttgc
    41341 acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag cgtgctctgc
    41401 caaccaggaa tcggtttatt cactacaccc tgcccacgct cctcacgcca ttcgcgcatt
    41461 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    41521 gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    41581 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    41641 cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    41701 agatcgtgtg gcccggcgct attgctacga tgtttatcca ttcttagcga ggctgcggtg
    41761 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    41821 aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    41881 gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca
    41941 aagtgactgg cggtgctgga atgtacaaaa accgcatcgc aactggcggc aaggctggat
    42001 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    42061 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    42121 ttttgcgcaa tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    42181 caggtgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    42241 ttcgacgcgg gcctgtaaat caccgtcaac cgcgttcgac atatccgtta ccgccggctg
    42301 atcctctacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    42361 ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc
    42421 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg
    42481 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagctt
    42541 cagatcgcca aattctgaat tacaaaaacg ttgttcatat ttggtgactc gattaccaaa
    42601 gccactcacc gtgcgcagat aatggcgttt taccagatta tccagttgct cctgcacctc
    42661 ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgttactac
    42721 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcagcc
    42781 aatcacgcgg gcttccagag cagtaagttg atatttcatg agattctcct tagcggccgg
    42841 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    42901 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgtgccagt aaatcaccgc
    42961 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    43021 tataacgaat ggctgcggtc agagcttcaa acatgagtcc tttgccctgc catttttgcc
    43081 caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg
    43141 caacgccaat aatctctttt tcgtccgggt cgaataaacc aaagtagaaa gctgaacctt
    43201 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga tagcagtggc
    43261 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    43321 ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    43381 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    43441 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtcccaaa
    43501 tgccattatc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    43561 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    43621 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg aggagcctgg
    43681 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    43741 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    43801 ctctcggtct acgccaattt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    43861 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    43921 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    43981 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    44041 aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    44101 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    44161 cagttctatt tgtgctgtgt gcggcgttca atgcgtggtt gttaccagca tggaaactct
    44221 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    44281 tcacctatgt tctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    44341 tgccaattat ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca
    44401 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    44461 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    44521 tgggcatggt cagcggcctg caacaacttt ttaccctgat ttgtctgttt tatatcgggt
    44581 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    44641 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggtggcgct attggttata
    44701 tcggtggcgg ctggctgttt gacctgggca aatcggcgca ccaaccagag cttccgtgga
    44761 tgatgctggg cattattggc atcttcactt tccttgcgct gggctggcag tttagccaga
    44821 aacgcgccgc gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc
    44881 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    44941 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    45001 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    45061 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc
    45121 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    45181 aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca
    45241 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    45301 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    45361 tcagcgatga tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    45421 aactttcgga agatgatatt cagctatttc cactactgcg taatctgacg ctggtagccg
    45481 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    45541 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg
    45601 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    45661 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    45721 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    45781 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    45841 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acctgaccag ccaaattggt
    45901 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    45961 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    46021 gaaaaaggtg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    46081 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    46141 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga agcaatggcg
    46201 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc atttactgat
    46261 taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    46321 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    46381 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    46441 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc
    46501 aatctggctc cgcccgtgac caccgttgag gctgccgtgg cgtatcgcca acgtattctt
    46561 gacgccgtac ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg
    46621 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    46681 tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcgattga cgccatcatg
    46741 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    46801 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaaccg
    46861 ctgcgccagc gcctgactgc actgaaagtc gtttttgaac acatcaccac caaagacgca
    46921 gccgactatg tccgtgacgg aaatgaacgg ctggctgcta ccatcactcc gcaacatctg
    46981 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtctaccc
    47041 atcctcaaac gcaatattca ccaacaggca ctgcgtgaac tggtcgccag cggttttaat
    47101 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcacctgc
    47161 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    47221 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaatggccc gcagttctat
    47281 gggttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcatca ggttgctgaa
    47341 agcatcgcac tgacagatga cacgctggtg ccattcctcg ccggggaaac ggtacgctgg
    47401 tccgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa
    47461 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    47521 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    47581 tgcgtttcct gataatgaag gccacgtatc ggtacgttac gccgcagcga ataatttatc
    47641 ggttattggc gcgacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    47701 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    47761 gtcgccccgg cttttttatt ttacttacga attttgagta caaaatttct acacttcctc
    47821 aaaataattg cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata
    47881 aatcttaaaa gcagaaattt atctgtttcc ccctttaaaa attcggtgaa tacccttact
    47941 tattggtata ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta
    48001 gtaacacgaa ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc
    48061 tcgtagggtg ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact
    48121 accagacccc aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa
    48181 ccactgatgt tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat
    48241 ccggtgattt cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa
    48301 aaggcacctc accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac
    48361 tctgcttaaa ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca
    48421 gcggttccgt aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctta
    48481 tgaccgacgc ccatatgcca ccacatcaac acggcagcca ccacggcgat acgcgattaa
    48541 ttcgccatgc ttatggtgaa ggcgaaaagt atgtcccact ggtcctccgc gcgcaaacac
    48601 tgtgggatga gctctcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca
    48661 ttaacctcgg cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt
    48721 ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac
    48781 gcgtcccgga caattacatc ggcttatttg agactgattc cggttttttg cgcagcgaac
    48841 tggcgattaa aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact
    48901 gcccggtcac cgcaattcgt catgacgatg atggcgtaac tattgaaacg gctgacggtg
    48961 agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg
    49021 agctgcctgt ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata
    49081 gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg
    49141 gttttccggc agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc
    49201 attcagcgga tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc
    49261 cgttcttgcg caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct
    49321 atgataattc gcctgacgaa gattttatta tcgataccct acccggccac gataatacac
    49381 tgctcattac cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag
    49441 ctgccgattt tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc
    49501 gcttccaata atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat
    49561 gcgtcggctg ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt
    49621 tctctgggga ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc
    49681 actgacataa actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgag
    49741 attattcctg cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat
    49801 gaagattctt tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg
    49861 ttatttttgt gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgtt
    49921 ttggatgcac ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc
    49981 ttccattggc taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc
    50041 acgctcagtt attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt
    50101 attttgttaa tgatggggct ggttattcgc gttctgtggc gcgtcatatc tccaccgccg
    50161 ggtccgctgc cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca
    50221 ttgtacctat tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt
    50281 aaaccgatca gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca
    50341 caggcagact ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtattgtcc
    50401 gttatgcacg gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag
    50461 cgcatgctgg gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt
    50521 ggtttaacct tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa
    50581 attgacaaag aaggtcagca cgcctttgtt aacttccgca tccagcatct tggctatagc
    50641 tggttatacg gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg
    50701 gctgccgata aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa
    50761 cgcgataaac atcttcgcag tgcggatttt ctcaataccg caaaatatcc acaggcaaca
    50821 ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg
    50881 ctgaatggtg taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac
    50941 ccatggggtg gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc
    51001 aatatcaaga cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa
    51061 ggcgtacagc agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt
    51121 tattctgttg gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag
    51181 atcttattgc cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg
    51241 ctttcttcgc agcaaatctc actacaacaa cctttgtatt tttccgcaca tgctggacac
    51301 tgaataaaca gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgca
    51361 ccgcactggt ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca
    51421 aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg
    51481 tattcaataa ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag
    51541 gcactggcct tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta
    51601 tgtgcctgca tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt
    51661 tcgagcgcgt tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat
    51721 agtgcatcgg gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca
    51781 ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa cgatacgatc gcggaccttc
    51841 atgcgcagta cccagaaaga tttcccatca tcatccaacg cgatattcag gcgtaaaccc
    51901 tctaaagccg ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgctggt
    51961 acgctgattt gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc
    52021 gcggtaaaca gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac
    52081 ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg
    52141 tcgttggaaa tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag
    52201 taaaaatccg cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta
    52261 catttgcgca ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag
    52321 ccacaaattt tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgaataggtt
    52381 gaaaaacagg attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg
    52441 ttattggtta acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc
    52501 ggttatctac cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg
    52561 cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg
    52621 ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg ggactgatgg aaactggcat
    52681 ggcgtggttc tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca
    52741 cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac
    52801 actggagctg ggtgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc
    52861 gaacgataat ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc
    52921 gatgctcgac cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt
    52981 ctggtacgca ccggatcatg attacggccc gcgctcaagc gttttcgtcc ctttatttgc
    53041 cgttgagcag gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg
    53101 tctggtgccc ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct
    53161 gccgccagag tgttctccgc cgctggatga tgccgaaact accgccgcgt ggatgaacaa
    53221 agtggtcgaa aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt
    53281 taaaacacgc ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag
    53341 aatggcatcc ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat
    53401 tgcgccccct ggaagtcggg cgcataatta gtgtgcttat cttttcttct tatgttcacc
    53461 gcgcctggcg caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa
    53521 ctggaaacga aacctgatcg tcgcctggct aggctgtttt cttaccggtg ccgccttcag
    53581 tctggtaatg cccttcttac ccctctacgt tgagcagctt ggcgttaccg gccactccgc
    53641 cctgaatatg tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc
    53701 accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc
    53761 tctcggcatg ggcatcgtga tggtgttgat ggggctggca caaaatatct ggcagttttt
    53821 gatccttcgg gcgcttcttg ggttacttgg cggatttgtc cccaacgcta atgctcttat
    53881 cgccacacaa gtaccgcgta ataaaagcgg ctgggcactg ggtacgctct ccacaggcgg
    53941 cgttagcggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt
    54001 acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt
    54061 ctgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt
    54121 ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacta cgttaatcat
    54181 ccaagtggcg acgggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg
    54241 taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc
    54301 tctgctgagt gcaccacgac tcggcaaact tggcgatcga atcggccccg aaaagatcct
    54361 gattacggcg ctgatcttct ctgtactgct gttgatccca atgtcatacg ttcagacgcc
    54421 attacaactt gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc
    54481 cgtacagaca ctgttggttt acaactcgag caaccaaatc gccggacgta tcttcagcta
    54541 taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc
    54601 agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt tattcaacgc
    54661 agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt
    54721 cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac
    54781 tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac
    54841 gcgaagaatt tcttgcagac aaccccggca tcgacgccga agacgcgaat gtgcaacagt
    54901 tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg
    54961 ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg
    55021 tttttgatgg cgactatgat gagatagaga tccgccagga gtggcaggaa gagaatacat
    55081 tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac
    55141 gcgcagcagc tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg
    55201 atcggcagca gtaaagtatc aaaaaccagg gagaatggca gatcaaggat tgtgacataa
    55261 cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt gccatgcccc
    55321 tgccccggta tagtgcggct aatgatgctg ccacagccac tcagcaaggt caccatgatg
    55381 ctcaccacaa ttaaacgcat ttgatacctc ttccgtaaaa aaataccggc ataacgccgg
    55441 tatttttatt ggctcgttgt ttttgccaga tgcgacgtga acgccttacc taacctacaa
    55501 aatcttgcaa aatcaataaa ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag
    55561 gcaactacgt ttttattgcg aagccgcatc cggtttacgc aatgccagtg gattgagctt
    55621 tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc agacgcggaa
    55681 atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat cgcgattcag
    55741 cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga tttccagcac
    55801 cttactggcg cggtgacgcg cggtcgccat tgcggtggcc agagcgttaa atgacgggtt
    55861 aaacactgcg tgcataaaac catcatcaag ggagcgttga cggttcatct caaggaaccg
    55921 atcggtatca accagtacct gcggcggtga atactcttcc gggatcagga acagtttcca
    55981 gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa acggtgacag
    56041 gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca accacgccat
    56101 cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga acgcttcacc
    56161 ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc agccaaggaa
    56221 cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca gcacggaaaa
    56281 cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccatatt ctttcgttcc
    56341 tttgcaccaa ataagcaaaa tgctcaatag ctttggcagg aacaacagca ccatggtcga
    56401 agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga acaactgccg
    56461 tggttgcagg aagtattgcg gttcggttaa cgcatgcact acctgcaatg cagtagagag
    56521 cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg tcaggaacac
    56581 cgcacggtga accgggtgca tacccttaac caggaacaga cggaagttca tcaggttacc
    56641 gtggcaccag cggcggtcac gttttagctc atcaagtaag ttaggcggta attcttcata
    56701 agaacctggg agatcgtaag caatccagac tccccaacct gcacggcgca tcaacgccgc
    56761 ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg gcagcggagc
    56821 cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc cccagtagtg
    56881 cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat acacgcgggt
    56941 cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg gcgacgactg
    57001 aatgatcccg gcgttcgggt tggcttccat caagcgcacc aggccgcata aacaatcacc
    57061 ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc cccagcgacg
    57121 gcagaagtca tcgatattac cgcttttacg cttcacgcga cgacggcggc ggcgatagaa
    57181 aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt gctctgcgac
    57241 gcagatatcc gggttataac tgtcactcag aatgtagaca tcaaagtgtt tggcattccc
    57301 ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt tcacgtcttc
    57361 gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt catcgccaac
    57421 tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga agcccattaa
    57481 cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga tcaggatacc
    57541 ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct gaccaaccat
    57601 atccaaagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca tataccaggt
    57661 cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga tggtaccgac
    57721 ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca gatagcgcgg
    57781 cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg tacgccacgg
    57841 gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttaagctgat cgcgcccttc
    57901 gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt ccagacgcgc
    57961 ctttaccgag ccttgcgggg agtcatcctc ccgcgcccag gtgcggtgtt cggcatccag
    58021 cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat
    58081 gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg tagctccagg
    58141 tttcactcaa cgtctgatcg gcattcacca gcgcagctcg catttcagtg gttttcttgg
    58201 catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg ttatagcgca
    58261 ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg accggggtat
    58321 cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg atagtaccgt
    58381 caggctggcg gatcaggttc gactgcttca catcccccgt tgaacgacgc gtttgttgca
    58441 cccatgcgtt atctggtgca tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt
    58501 taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa gcgacgatgt
    58561 tatcgttagt ttcatcgttg gttggaattt ccaccagctc aacgctgcct ttgccccact
    58621 cccctttcgg agtcacccat gcgcttggac ggagatcgta acgatcatcg agatcttcaa
    58681 agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt tccatggaga
    58741 agctgctgac cgctaaatgt ttcgggttat tcaacggacg ccagatccat tcgccattac
    58801 cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta tttgcaggcg
    58861 acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc agtttgccga
    58921 ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc cctggcataa
    58981 ctacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac
    59041 gtttatcagt tggttttgga cgctcgatcc agaactcttt gaagcgtgga aattcttcac
    59101 ccgatggcaa ggcggtatca attgccagac cgcgggcaga aaggccataa acctgacctg
    59161 caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg tttttatctt
    59221 tgctgttgct cgggtaaagc actttaaaac cggcaaaacc aaggtctttt accgtgtctt
    59281 tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt cgtttgactg
    59341 cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacatcccc tgatggtaga
    59401 actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga ttaaactgga
    59461 tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg
    59521 cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca atactgaaag
    59581 cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa cgcattttca
    59641 tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg cttatatcga
    59701 tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt atcgttcagc
    59761 acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt gcttctccta
    59821 aagacagaag catcgacttc gtgtagggtt tgccagcaaa tttatttatc aaccgtctta
    59881 cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac ttgaagcctg
    59941 cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat aacccgacag
    60001 ggataagacg aaaaatccga gattagttaa ccatatatga atccagtacc cgcgcaacgt
    60061 gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt tagggatccc ttttcatatt
    60121 tctttaatct attcgagcca tacatggcat gtgaatagcg ccgaaccgtc attgtggctg
    60181 acccttttta atgacttcat ccactcgttc cgcatgcagg tatttttcgt tatatccggc
    60241 tacttttcct acatgctttt tttacgctat cccttgaaaa aatggtggaa agtacgtgtc
    60301 gaacgtgtag gtatcccgat gttaacagcc atccccctac tgacattgcc gcaatttatt
    60361 atgctgcaat acgtcaaagg gaaagcggaa agttggcctg ggctgtcatt gtatgacaaa
    60421 tataatacgt tagcctggga attaatatcg cacctgtggt ttttactggt gttagtggtc
    60481 atgacgacgc tgtgcgtatg gatatttaaa cgcatcagaa ataatttaga aaattctgat
    60541 aaaacgaata aaaaattctc gatggtaaaa ctatcgctga tttttttgtg cctcggcatc
    60601 ggttatgcgg taataagaag aacgattttt attgtgtatc cgcccattct gagtaatggc
    60661 atgttcaatt ttattgtcat gcaaacgcta ttttatttgc cgttctttat cctcggcgca
    60721 ctggctttca ttttccctca tcttaaagcc ttgtttacca cgccgtctcg tggctgtacc
    60781 ctcgctgcag cattggcgtt tgtcgcttat ttactcaacc agcgctatgg cagtggcgat
    60841 gcctggatgt acgaaaccga gtcggtgatc accatggtcc tcggtctgtg gatggtgaat
    60901 gtggtcttct cctttggcca ccgtttgctt aacttccagt cagcgcgggt gacttacttt
    60961 gttaacgcat cgctatttat ctatctggtt caccacccgt taacgctgtt tttcggcgcg
    61021 tacattacac cgcacatcac ctccaactgg cttggttttc tctgtggcct gatattcgta
    61081 gtagggattg cgataattct gtatgaaatt catttacgca tcccattact gaagtttttg
    61141 ttctctggta aaccggttgt taagcgtgag aacgataaag caccagcccg ttaagccgcg
    61201 tttacaataa ccattccacg ggcaatatcg acgccagtct gaccataacc cgcttccaga
    61261 aactggtggc gggttctttt ttgagaacaa tctctttctt tgcatgacga tcaacccagt
    61321 tgatccgccc ccacctgtcc agacggagct gccaggccgc atcatactgg ctctgaataa
    61381 agcgtttatc aattaactgt gccagcgttt cgctctctat cacgaagccc atttcagtat
    61441 tgagcaatgt cgaacgtgga tcgaaattga aagagccgat aaacaccgtt ttaccatcga
    61501 tgctaaaggt tttagcatgc aggctggctc cggaattacc ggttatgccg cgatcgtgta
    61561 acgtactact ttgttcacgc gtcggcttga gttcatataa ttccacgcca tagcggagca
    61621 attttttgcg ccagcgcgcg tatccggcat ggacgacagc aacatcgtta gcggcaagag
    61681 aattggttag gatcgcaatc tttacccctt ttctcaccat ccgtaagagt tgcgccacac
    61741 ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat gcgttcactg ggtgagccca
    61801 tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt tgccttgccc tcccctttcg
    61861 ccggatcatc acttaataaa cgtgttttcg cccagataag cggcaacgtt ccatcaacca
    61921 gatgatttat aaacggactg gattccattt tgcgtaagta acgatgcgtc atggcatcgt
    61981 tatgccagga ggcgggtaac tcgatgcgat ccgccatttc accttccggg acatccagca
    62041 cctgctgtaa gggtgaaacc gatttgcaat accagtagcg ggcgaaatca tcggcaacgt
    62101 cctctactac gggtcctatt gccatgacat ctaaatccga aaaaagtggc tcttcccctg
    62161 ccccaaaata ggcatcacca atatttcgcc cccctaccag cgtcaccacg ccatcgacag
    62221 tgaaactttt attgtgcata cggcgattga gacgggaaaa gtcggtgata taaccaagcg
    62281 gacgcagcaa gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg ggatggctgt
    62341 caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc aacaaacgga
    62401 cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa cagtaaccga cccgacatat
    62461 cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc tgccatttcg gccagacgat
    62521 accgggcggc aaacgcatcc agacttttct ccagagggaa aagaccacac tgaccgggat
    62581 gttgcgaaca cagtggcagc accgcgctcg ccagccgggg caaatcattc atctccttgt
    62641 tgggtaagga gtctttcgta gaggtgggag ttttcttcat cataacagac aaagtatacc
    62701 tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg tttttactgc gatttcagcc
    62761 gctgccgcac gagggtaacc ataaacccca gtactgattg caggaaaagc cactgacgta
    62821 tagctgtttg ccgccaccag tcgcaggcta ttgagatagg catcctgcaa aagctggtct
    62881 tcgttttgtt caccaccacg ccagactggc ccgacggtgt gcactacggc tttagcagga
    62941 agatcgcctg caagcgtaat aacggcatgc cccgtagggc aatcgccctg ctgttgccta
    63001 acttttaaac aggcatccag cagggccgga cccgctgcgc gatgaatggc cccatcgacg
    63061 ccgccgcctc ccattaatga cggattagcc gcattcacaa tcacatcaac ggccagtttg
    63121 gtaatatcac cctgcacaac atgaatacgc gttttcataa cgcctcctta caccttcttg
    63181 tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctgctca gcaacgtgag
    63241 ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct gtttatcaag
    63301 atactgcact tttactgagg ctacttgcga gtttttaaca ttgccgctca ttaacgcttt
    63361 gctggaatag gtttttcccg gaacagaacc attctgtggg cagttcattt caacgggtga
    63421 tgactgggtg ctgacgtcac atgccggttc tacaatttgg ccataaaaat ggatcacgcc
    63481 cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa aacaaactgc cgagcataag
    63541 agagtttaaa aaaggtctga acatttattc tatttcctca atgattagtc atccttgagg
    63601 gttgtgttat ccatactttc aggttggctg attcgcagca gaccattctc tccagattca
    63661 tcttatgctc gatatttcaa caaattaaga cttttctgaa gagggcggcc attgttgtga
    63721 taaatgaagt gactgtccat cagaaacagt aacaactatt ttcacccgat cgtccgggga
    63781 aatatttaaa ctcaacttcg tcaaagcaat gggttgatta gcaggcaatg aaagggtctt
    63841 ttcttgcttc gtctgacttt gccctgaact gccttcgcgc agggacaata tttgtactct
    63901 gcacagacaa gattgagtaa gagtgacttc aggaataatg gtatacacat ccccttgctg
    63961 ggtcgtatta aaggttatct gactggaaag tgccgcaagg agtaataacg cattcatatt
    64021 cttctcccga aaaaaaacag ggcttgcgcc ctgtttctgt aatacaaatg atgtattagt
    64081 actgatgagc ggtcgcgttg ttaccaaagc caacctgagt cacgttgacg gaggagttag
    64141 atgcagtctg gtcaactgca gcaccgttgc caccaccgaa ctgtttaacc gtcatttcag
    64201 aatttttgcc gttccactga tcaagagtag cgctgttacc gaagccacgt tgggtcagat
    64261 cgattgagct gtcatccgag ccctgaccaa catctgcacc attaccgccg ccatgctggg
    64321 taatagtcaa gtcagagtta cgggcatcag tttgcagagc aagtgcagag ttaccgccac
    64381 cgtactggta aatgttcagc tcagaatttg ggccgctatt attaccgcca ccaccgtggt
    64441 taccgccgcc gccgtactga ggaacaacac ctgccagagc gctaccggag aatacgattg
    64501 ctgcaattgc tgctactttt aaaagtttca tgtaaaaccc ccatcggatt gatttaaaag
    64561 tcgaatggaa attaacgttg tgtcacgcga atagccattt gcgactgtct ctgcactaca
    64621 attgccgttt tttgagtacc atactgtgta atatttgctt tattaccaga acctttctgg
    64681 ataatcatcg cagtattacc ataagcacct tgcgaaatac tggcatcgtt ggcactgccc
    64741 gcctgatcaa tatatgcaag gttataatct cctgtctggt caatctttgc ccggttgcta
    64801 ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa ctgagcacta
    64861 ttattagtcc cagcttgacc aattatggct gcctgattaa atgaagactt actcaattca
    64921 tttaccgcga agttatattc tgaattagct aaatcataac ctgctgcggc tgcaatccca
    64981 ggcgcaccca gtattgttaa catcataaat aacaatttgt ttttcatgtt gtcaccctgg
    65041 acctggtcgt acatttaaga aattaaatca tttcaacttg gttgttaacg caacctgtat
    65101 tttgttaacg ctgcgttacg atggaaagta tgtctgcgga aatattttta ataactcacc
    65161 cgcgcgttgt atttttcttt ttttgttcat accaaagtat taaaagccac gtaaatacgc
    65221 tgttatctac gcaaaaaata ttttgttttc ttttaaatct ccgtttccgc tatcaaaaat
    65281 caccagacag tcattcttct tgcccgtcgc tgattgctgc gatatgtctt gcgcacaagc
    65341 cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac ggaggtgtgg agaaaaaaca
    65401 acaacttttt acatgacgaa aggac
//
LOCUS       DAZGCQ010000014        61259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000014 DAZGCQ010000000
VERSION     DAZGCQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.014"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(73..3222)
                     /gene="acrB"
                     /locus_tag="ACHK07_000994"
     CDS             complement(73..3222)
                     /gene="acrB"
                     /locus_tag="ACHK07_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO8101366.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3245..4438)
                     /gene="acrA"
                     /locus_tag="ACHK07_000995"
     CDS             complement(3245..4438)
                     /gene="acrA"
                     /locus_tag="ACHK07_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO8101367.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4580..5227
                     /gene="acrR"
                     /locus_tag="ACHK07_000996"
     CDS             4580..5227
                     /gene="acrR"
                     /locus_tag="ACHK07_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO8101368.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5355..8717
                     /gene="mscK"
                     /locus_tag="ACHK07_000997"
     CDS             5355..8717
                     /gene="mscK"
                     /locus_tag="ACHK07_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO8101369.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKIKVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8778..8876
                     /locus_tag="ACHK07_000998"
                     /pseudo
     CDS             8778..8876
                     /locus_tag="ACHK07_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8929..9090)
                     /gene="rsmS"
                     /locus_tag="ACHK07_000999"
     CDS             complement(8929..9090)
                     /gene="rsmS"
                     /locus_tag="ACHK07_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO8101370.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9104..9631)
                     /gene="priC"
                     /locus_tag="ACHK07_001000"
     CDS             complement(9104..9631)
                     /gene="priC"
                     /locus_tag="ACHK07_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO8101371.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9701..10078
                     /gene="ybaN"
                     /locus_tag="ACHK07_001001"
     CDS             9701..10078
                     /gene="ybaN"
                     /locus_tag="ACHK07_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO8101372.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10231..10782
                     /gene="apt"
                     /locus_tag="ACHK07_001002"
     CDS             10231..10782
                     /gene="apt"
                     /locus_tag="ACHK07_001002"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO8101373.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10911..12842
                     /gene="dnaX"
                     /locus_tag="ACHK07_001003"
     CDS             10911..12842
                     /gene="dnaX"
                     /locus_tag="ACHK07_001003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO8101374.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12175..12239
                     /gene="dnaX"
                     /locus_tag="ACHK07_001003"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12895..13224
                     /gene="ybaB"
                     /locus_tag="ACHK07_001004"
     CDS             12895..13224
                     /gene="ybaB"
                     /locus_tag="ACHK07_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO8101375.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13224..13829
                     /gene="recR"
                     /locus_tag="ACHK07_001005"
     CDS             13224..13829
                     /gene="recR"
                     /locus_tag="ACHK07_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO8101376.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13939..15813
                     /gene="htpG"
                     /locus_tag="ACHK07_001006"
     CDS             13939..15813
                     /gene="htpG"
                     /locus_tag="ACHK07_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO8101377.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15994..16638
                     /gene="adk"
                     /locus_tag="ACHK07_001007"
     CDS             15994..16638
                     /gene="adk"
                     /locus_tag="ACHK07_001007"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO8101378.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16770..17732
                     /gene="hemH"
                     /locus_tag="ACHK07_001008"
     CDS             16770..17732
                     /gene="hemH"
                     /locus_tag="ACHK07_001008"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO8101379.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17729..18688)
                     /gene="aes"
                     /locus_tag="ACHK07_001009"
     CDS             complement(17729..18688)
                     /gene="aes"
                     /locus_tag="ACHK07_001009"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO8101380.1"
                     /translation="MKPENKLPVLDLISAEMKIVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNYADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18840..20144
                     /gene="gsk"
                     /locus_tag="ACHK07_001010"
     CDS             18840..20144
                     /gene="gsk"
                     /locus_tag="ACHK07_001010"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO8101381.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20274..21950)
                     /gene="ybaL"
                     /locus_tag="ACHK07_001011"
     CDS             complement(20274..21950)
                     /gene="ybaL"
                     /locus_tag="ACHK07_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO8101382.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22188..23408)
                     /gene="fsr"
                     /locus_tag="ACHK07_001012"
     CDS             complement(22188..23408)
                     /gene="fsr"
                     /locus_tag="ACHK07_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO8101383.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23626..25278
                     /gene="ushA"
                     /locus_tag="ACHK07_001013"
     CDS             23626..25278
                     /gene="ushA"
                     /locus_tag="ACHK07_001013"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8101384.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARVLPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QLDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25315..25794)
                     /gene="ybaK"
                     /locus_tag="ACHK07_001014"
     CDS             complement(25315..25794)
                     /gene="ybaK"
                     /locus_tag="ACHK07_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO8101385.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25998..26792)
                     /gene="ybaP"
                     /locus_tag="ACHK07_001015"
     CDS             complement(25998..26792)
                     /gene="ybaP"
                     /locus_tag="ACHK07_001015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO8101386.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTNDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26930..27271
                     /gene="ybaQ"
                     /locus_tag="ACHK07_001016"
     CDS             26930..27271
                     /gene="ybaQ"
                     /locus_tag="ACHK07_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8101387.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLALKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27486..29990)
                     /gene="copA"
                     /locus_tag="ACHK07_001017"
     CDS             complement(27486..29990)
                     /gene="copA"
                     /locus_tag="ACHK07_001017"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO8101388.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADSDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30252..31184
                     /gene="glsA"
                     /locus_tag="ACHK07_001018"
     CDS             30252..31184
                     /gene="glsA"
                     /locus_tag="ACHK07_001018"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO8101389.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31187..32479
                     /gene="ybaT"
                     /locus_tag="ACHK07_001019"
     CDS             31187..32479
                     /gene="ybaT"
                     /locus_tag="ACHK07_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8101390.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32817..34172
                     /locus_tag="ACHK07_001020"
     CDS             32817..34172
                     /locus_tag="ACHK07_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO8101391.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMVSAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            34275..36299
                     /locus_tag="ACHK07_001021"
     CDS             34275..36299
                     /locus_tag="ACHK07_001021"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8101392.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTTVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGEVAITASVTDLSGNTGNTSRTITVDSQAPA"
     gene            36357..55052
                     /locus_tag="ACHK07_001022"
     CDS             36357..55052
                     /locus_tag="ACHK07_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8101393.1"
                     /translation="MPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVD
                     ALADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHIQAQII
                     SGTVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDK
                     AGNTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTL
                     NGQNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSTTASHNVLVDSAL
                     PGVTINPVATDDIINAAEAGVAQTISGQVTGAAVGDAVTVTLGGNNYTTTVQAGLSWS
                     VDVPADAIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNI
                     IEHGQALVVTGSSLGLAEGTPLTVTINDVEYTTAVQADGSWSVGITAAQVSAWPAGAV
                     NIAVSGESSAGNPISITHPVTVDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNV
                     EAGQTVTVTFGGKNYTASVAGDGSWTATVPAADLAALPEGSASAQASVSNINGNSASA
                     VHSYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQT
                     YQATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLT
                     INTIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVP
                     AADVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKG
                     ADLQISGTSNQPAGTTITLTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTAN
                     VTDSAGNSNSASHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDT
                     VTVTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGELTVNAWVTNGVGNTGSGSRDIT
                     IDANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLA
                     DGTWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGD
                     DVINAAEKGADLTLSGSTSGVEPGQTVTVTFDGKTYTATVAGDGSWTTTVPAADLAAL
                     RDGDATVQASVSTINGNTASATHVYSVDATTPTLTINTIATDDILNAAEAGNPLTISG
                     SSTAEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVNDKAGN
                     PVSATHGLAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNS
                     KTYTTTLDASGNWSVGVPLSDVTALGSGPQNITATITDAAGNSDDASRTVTVNLAAPT
                     IGINTIATDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDASGNWSATV
                     PASAVSALGEANYTVTANVTDKAGNSNSASHNVLVNSALPAVTINAVATDDIINADES
                     DNAQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSISVPAADIQALGNGDLTIN
                     ALVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAG
                     TALTVEINNVTYAATVLADGTWSLGVPAVDVSNWPAGTVNITVSGTNSAGTTSTITHP
                     VTVDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTV
                     EANGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIA
                     SDDTLNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSG
                     LTASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVS
                     GTSTGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRA
                     GNSDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLIISGGSSGLATGTTVTVML
                     NGLAYSATTDGSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGITTHTVNVESLL
                     PGVIINTVAGDDIINAAEIGVNQTISGQVTGTAAAGDTVTVTLGGNQYVATVQPDLSW
                     SVSVPANVLQALGNGELTISASVTNSANNTGTTTHDIVIDANLPGLRVDTVAGDDVIN
                     SIEHTQALVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGT
                     VNIAVSGTNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSD
                     VEAGQTITVIFGGKSYTTTVAADNTWGLTIPAADLAALPDGAANVQASVSSVAGNSAQ
                     ATHVYSVDATAPSVTINTIAADDILNAAEAGSALIISGTSTTEAGQTVTVTLNGVNYS
                     GNVQADGSWSVSVPTGDLANLTASSYTVNASVSDKAGNPASATHNLTVDLAAPVVTIN
                     TVAGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPAS
                     VISALAQGDVNISATVTDSAGNSGTASHVVSVALGAPVLAINTIAVDDIINATEKGAD
                     LAISGTSDQPAGTQITVTLNGQNYTTTADASGNWSVTVPASAVGTLGEATYTVTASAT
                     DADGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVT
                     VTLGGATYTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITID
                     ANLPGLRVDTVAGDDVVNIIEHGQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADG
                     SWSVGVPAADVSAWPAGTVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINAITVDDV
                     INAAEKGAALTLSGSTSGVEAGQTVTVNFGGKTYTASVATNGSWSTTVPAADMAALRD
                     GDASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSS
                     TAEAGQTVTVTLNGENYTGTVQTDGSWSVSVPQADVSALTASNYTVSAAVNDKAGNPA
                     SVNHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNT
                     FTTVLDASGNWSVGVPASVVSALANGTVTINASVTDVAGNSGSATHQVTVNTGLPTIT
                     FNAISGDNVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVP
                     VSDLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAG
                     ADQTISGVVTRAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITA
                     SVTNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGA
                     VLTVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLTVEVAGQSSADNPVSVSHPF
                     TVDLTAVAISINTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVA
                     ANGSWSKTVPVADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIAS
                     DDILNATEAGSPLTISGSSTAETGQTVTVTLNGATYTGTVQADGGWSVSVPTSALGAL
                     NASNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISG
                     TSTGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAG
                     NSDDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNG
                     QNYSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPG
                     ITINPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQPDLSWNVS
                     VPAADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIE
                     HSQAQVITGSSSGFAAGTALTVVINNQTYAATVLVNGTWSVGVPAADVSNWPAGTLNI
                     TVSGANSAGTQTSITHPLTVDLTTVAISINAITPDDVINAAEKGAALTLSGSTSGVEA
                     GQAVTVTFGGKTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATH
                     EYSVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTV
                     QADGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVA
                     GDDVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVIS
                     GLADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTL
                     SGTGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQD
                     SAGNSATASRSVAVDLTASVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVT
                     FGGKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTV
                     APTVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWSATVGSGGSWS
                     VFIPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNA
                     AEHGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAY
                     VINASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNG
                     SLTAALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNV
                     GATDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGATLSAG
                     EFAQISIDGGTTWQNLSVSGLTWSYLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVT
                     VDTTAPLASKTIAIASISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGG
                     VTWTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAA
                     DGLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTSLTLTGSLGAGLASGEVAQISLDGGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPGIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            55056..57218
                     /locus_tag="ACHK07_001023"
     CDS             55056..57218
                     /locus_tag="ACHK07_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO8101394.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIVATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            57215..58390
                     /locus_tag="ACHK07_001024"
     CDS             57215..58390
                     /locus_tag="ACHK07_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO8101395.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            58387..58794
                     /gene="cueR"
                     /locus_tag="ACHK07_001025"
     CDS             58387..58794
                     /gene="cueR"
                     /locus_tag="ACHK07_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO8101396.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(58798..59001)
                     /locus_tag="ACHK07_001026"
     CDS             complement(58798..59001)
                     /locus_tag="ACHK07_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101397.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(58998..59420)
                     /locus_tag="ACHK07_001027"
     CDS             complement(58998..59420)
                     /locus_tag="ACHK07_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101398.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(59521..>61259)
                     /locus_tag="ACHK07_001028"
     CDS             complement(59521..>61259)
                     /locus_tag="ACHK07_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8101399.1"
                     /translation="KSVKLSLLAVVVATIVSPSAFADDTVEAAATELTAIHPGMSHDE
                     IDQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPASTAVQTDSNITDQKQADRD
                     AAQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASAAH
                     ANTNANTALMNGLKLSGAVSDNKNQIEKNSSSIAEQQEQMALSEQNQIHRDAAQDSSI
                     QDAQHSANWASLKADDAHHAIMVAQTDIDANKSAISDTQKDVAAVRTDVSAMKDDVAD
                     VKASADHANANANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAATG
                     DVKTAARYQSMINALQTATADAQQSQSEAQQTQITEQQKQIAATEKTVAAIGDPQSTA
                     HYQKMINAKLTAQNEANERSTADQEQRINELASNVATQQQIDSVQYGEQIRNLAQDSA
                     QAHEQIESLTQDSAQTHQQLTNTQKRVADNSQQINSLNNNFSSLKHEVEDNRKEANAG
                     IASAVAIASQPQVKTGDFMMVSAGAGTFNNESAVSVGASFNAGIHTVIKAGVSADTQS
                     DFGAGVGVGYSF"
BASE COUNT        14450 a        16949 c        16950 g        12910 t
ORIGIN      
        1 aattactcct taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga
       61 ttacacgttg tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg
      121 gctaaagcgg cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt
      181 tgcggtcacc atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc
      241 accagtactg ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat
      301 cggacgtaaa cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt
      361 atccatcaag tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat
      421 ggttgtgagc aggcctacct ggaagtaaac gtcattggtc agcccacgga aggtggcagc
      481 cagcaacgca ccgataaccc ccagcggaac gaccagcata acggagaacg gaatcgacca
      541 gctctcgtac agcgccgcca gacacaggaa cacgacaatc aacgaaatcg cgtacagtga
      601 aggtgcctgg ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac
      661 accggtaggc agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt
      721 acccggtgcc gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg
      781 cggcgaaccg tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc
      841 agcagcacga acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga
      901 catgacgtaa actttcttca cacgaccgcg gtcgataaag tcgttcacat agctgccgcc
      961 ccatgcagcg cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt
     1021 ttcctggtcg atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct
     1081 ggtcaacata tcagggtgct tcgctgcttc tgcaagcaac tggttacgcg cctgagtcag
     1141 tttttcgtga ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcggtacc
     1201 cagttccacg atcgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa
     1261 agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc
     1321 ccagtccttc aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa
     1381 gccgttaacg gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt
     1441 tacctcattg agcactttct gtgtacgttc ctgcgttgca cctgctggca gctgaaccat
     1501 ggtcataaat acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag
     1561 ataggccatg ccgaccacga tgatcagata cagcaccagg taacgccctg tactgcgcag
     1621 aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca
     1681 gccgaagaag ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt
     1741 ggcacaaaga gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac
     1801 aatggtaata gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg
     1861 tacgaatacc gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat
     1921 cgacttacgg gtggcttctt ttggcggcaa accttcttcc gccatcacac gctcaacgtt
     1981 ttctaccaca acgatagcgt catccaccaa caggccgatg gcgagcacca tcccgaacat
     2041 tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac
     2101 caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat
     2161 aaccaggaac acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat
     2221 tttcacgaac ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg
     2281 ttccatcttc gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt
     2341 cgccagcttg atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta
     2401 gttctcacca cccagctcaa tcttcgccac atcacgcagc agcacgcggg aaccatcctg
     2461 attcactttc agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat
     2521 aatagaggcg ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc
     2581 aacctgggcg ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt
     2641 gttcagctca ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac
     2701 gtcacccacg cccgacgtac ggctgatggc atctttcata ttcgccgcca catagtcgga
     2761 gatatcctcc tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct
     2821 gctggatgat ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat
     2881 cgccagctgc agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc
     2941 aaaggtcagg gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag
     3001 gttatcgata ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt
     3061 cgcatcagcg ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata
     3121 ttgcgccacc ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat
     3181 cacccacgca aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt
     3241 taagttaaga cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag
     3301 cggtaacttc ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta
     3361 ctacgcgatc gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc
     3421 ttgcaacgat cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat
     3481 cgccacgcgg cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc
     3541 cttcttccag acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga
     3601 agatagcgcg tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca
     3661 gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc
     3721 cgttctcttg tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc
     3781 tggactgggt cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg
     3841 cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa
     3901 tcggagaggt gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg
     3961 ctgcagttac cgcagcattc gcctgttgcg catcagccag agcctgatcg tactcttgct
     4021 tactgatgta ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat
     4081 tggctgcagc ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg
     4141 tcgcaggatc aatctgatag agagagacac ctgcttcgat gtcgctacct tctttgaaat
     4201 tacgcttcag gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg
     4261 tgcgacccgg aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa
     4321 cggcgggcat ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta
     4381 agctgcctga gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat
     4441 gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc
     4501 tgcgtttata ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg
     4561 cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca
     4621 acacatcctc gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct
     4681 gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga
     4741 caagtcggat ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga
     4801 gcttgagtat caggcaaaat tccctggcga tccactctca gtattaagag agatattaat
     4861 tcatgttctt gaatccacgg tgacagaaga acggcgtcga ttattgatgg agattatatt
     4921 ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg
     4981 tctggaaagt tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt
     5041 gcctgcggat ttaatgacgc gtcgcgcagc aattattatg cgcggctata tttccggcct
     5101 gatggaaaac tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta
     5161 cgtcgccatc ttactggaga tgtatctcct gtgccccacg cttcgtaacc ctgccactaa
     5221 cgaataaccc tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg
     5281 gttcactgcg tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga
     5341 aaccttcatt catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt
     5401 cagcatttat tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt
     5461 catcgaatgg tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata
     5521 aacaaaaaga tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag
     5581 ccaccctcga taaaatcgat cgcgtaaaag aagagaccgt tcagctacgg caaaaagtcg
     5641 ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg
     5701 ataacgacga agaaacgcgc aaaattctga gcacgctttc gttgcgccag ctggaaactc
     5761 gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa cgatctagcg tcttataaca
     5821 gccagctggt ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt
     5881 cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct
     5941 tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg
     6001 accagcagcg taaaagcctg gaagggaata ccgtcttgca ggataccttg caaaagcaac
     6061 gtgattacgt gacggcgaac agcgcccgtc tggagcacca gttacaactg ttgcaagaag
     6121 cggtaaacag caagcgcctg actttaaccg aaaaaacggc gcaggaagcc gtctccccgg
     6181 atgaagccgc gcgtattcag gctaatccgc tggtgaagca ggaactggaa attaaccagc
     6241 agttaagtca gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca
     6301 ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga
     6361 ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc
     6421 tgccctcggc ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac
     6481 agtttgaagt taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac
     6541 tggaagaagg tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg
     6601 ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga
     6661 tggccattaa cctgcaaatc aaccagcagc agttaatgag tgtgtcgaaa aacctgaaat
     6721 ccatcctgac tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga
     6781 tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatt aaggtgaact
     6841 gggaaaaagc ctggcccgcc gtttttatcg ctttcctcgc tggtttgccg ctgctgttga
     6901 ttgccgggct gatccactgg cgtctggggt ggctgaaagc gtatcaacaa aaactggctt
     6961 ccgctgtggg ttccctgcgt aacgacagcc agctcaacac accaaaagcg atccttatcg
     7021 acctgatccg tgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga
     7081 ccatgcagct caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct
     7141 ggctggtgtt tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact
     7201 tcggcatgcc ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg
     7261 cgttgctgcc tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg
     7321 atgatgtgct ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat
     7381 ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctggtcacca
     7441 ttaccgtgct gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct
     7501 acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga
     7561 acttgctcta ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc
     7621 gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc
     7681 cggaagaacc caccattgca ctggaacagg ttaaccagca gacgctgcgt attaccatgt
     7741 tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg
     7801 tgttcagcta tctcgacagc attacgctgt ggcattacaa cggtactgaa gctggtgctg
     7861 cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg
     7921 cctgggcgtt gattcgcaac ctgcctggtt tgctggaagt gctggtgctc tcgcgactga
     7981 atatgcgcca gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgctg
     8041 ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg
     8101 cagcagcatt atccgtaggt ctcggttttg gtttacaaga aattttcggt aacttcgtct
     8161 ccggtttgat cattctattc gaacgtccgg tgcgtattgg cgatacggta accattggta
     8221 gcttctcggg gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc
     8281 gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt
     8341 tgactgacac tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg
     8401 aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac
     8461 caatgccgga agtcttcttt acggcgtttg gtgccagcac gttggatcat gagctgcgtc
     8521 tgtatgtgcg tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg
     8581 atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc
     8641 acaacgagaa gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gatgacccga
     8701 cgccagcggt agggtaataa cgaaaggggg cgacattttg ttgccccgag attgctaaca
     8761 aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca
     8821 aaaattcaat atattgcagg atcggtgtag gcctgataag cgtagcgcat caggcagttt
     8881 tgcgtttgcc cgcaacctta ggggacattt agccacccca tttatttctc acttttccgc
     8941 ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
     9001 cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga cggccagttt
     9061 gacatcgtcg ggggcatttt ccagtgacat actctctcca ttgctagcgg gttaaacgcg
     9121 ctaacctgtt ttcgattttt tccagcgcat ggcgacaccg cgccaggcgc gcttcatagg
     9181 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
     9241 ctctacgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
     9301 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggctg
     9361 tggcttcacg cgctatagcc tccagttgcg ccgccagatg ttctgccagc caggcgactt
     9421 gcggcagttg ctgttgctca actgcatgac gaagcgcagc cagattatcg cccgcctcat
     9481 cgagacaagc ctgcagtgtt gtcgcacggg tctgaaaaag gtgcctgtca aagcgggcac
     9541 ttagcgtggc aaattgcgct accggggcac aacgctggcg cagcgtggcg agctgtcctt
     9601 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
     9661 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
     9721 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
     9781 gacgacgccg tttatcctgc tagcggcctg gtgctttgcc cgttcttccc cgcgctttca
     9841 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
     9901 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
     9961 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
    10021 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
    10081 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
    10141 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
    10201 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
    10261 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
    10321 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
    10381 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
    10441 ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa
    10501 ctgccgcgtg aaactatcag tgaaacttac gatctggaat acggcaccga tcagctggag
    10561 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
    10621 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
    10681 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
    10741 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
    10801 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
    10861 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
    10921 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
    10981 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
    11041 ccggtacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
    11101 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
    11161 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagaca
    11221 ctcgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc
    11281 tcatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaactcttg
    11341 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgacccacag aaattgccgg
    11401 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
    11461 gccatcaact tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc
    11521 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
    11581 cgattgccag cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc
    11641 ttgacgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
    11701 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
    11761 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
    11821 acgacatggc cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
    11881 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
    11941 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc
    12001 cgctgcctga gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa
    12061 tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca ccgactgtac
    12121 cgctcccgga aaccaccagc caggtgctgg cggcgcgcca gcagttgcag cgcgtgcagg
    12181 gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgg ccggtgaata
    12241 acgctgcgct ggaaagactg gcttcggtca cggatcgcgt tcaggcgcgt ccggtgccat
    12301 cggcgctgga aaaagcgcca gctaaaaaag aagcgtatcg ctggaaggcg accactccag
    12361 tgatgcagca aaaagaagtg gtcgccacac cgaaggcgct gaaaaaagcg ctggaacatg
    12421 aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc cattgagcgc gacgcgtggg
    12481 cggctcaggt gagtcaactt tcgctaccaa aactggtcga acaggtggcc ttaaatgcct
    12541 ggaaagagga gagtgataac gcagtatgtc tgcatttgcg ctcctctcag cggcatttga
    12601 acaaccgcgg tgcacagcaa aaactggctg aagcgttgag catgttaaaa ggttcaacgg
    12661 ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg gagtggcgtc
    12721 aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg gataataata
    12781 ttcagaccct gcgtcgattc ttcgatgcgg agctggatga agaaagtatc cgccccattt
    12841 gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa acctatgttt
    12901 ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga aaaaatgcag
    12961 aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc aggtctggta
    13021 aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc gagcctgctg
    13081 gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga cgcagcacgt
    13141 cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat gcagctgccg
    13201 cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag cttatggaag
    13261 cactgcgctg tctgccgggc gttggcccga agtcggcgca gcgtatggcg ttcacgctgc
    13321 ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg gcgatgtcgg
    13381 aaatcggcca ctgcgccgat tgccgcactt tcaccgaaca ggaagtctgt aacatctgtt
    13441 cgaatccgcg tcgtcaggaa aacggtcaaa tctgcgtggt ggagagtccg gcggacattt
    13501 acgccattga gcagacgggg cagttttcag gtcgttattt tgtgttgatg ggacatctgt
    13561 caccgctgga cggcatcggt ccggatgata tcggccttga tcgtctggaa cagcgtctgg
    13621 cagaggaaaa aatcactgaa gtgatcctcg ccaccaaccc tacggttgaa ggtgaagcta
    13681 ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagccagc cgaatcgctc
    13741 atggcgtacc ggttggcggc gagctggaaa tggtcgatgg caccacgctg tcacactccc
    13801 ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc tgctctcgct
    13861 tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct taaaatggca
    13921 ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc agaagtgaaa
    13981 cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt cctgcgtgag
    14041 cttatctcta acgcctccga tgcggcggac aagctgcgtt tccgcgcgct ctctaacccg
    14101 gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa agacaagcgt
    14161 acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat tgaccatctg
    14221 gggactatcg ctaaatccgg cactaaatca ttccttgaat cgctgggttc tgaccaggcg
    14281 aaagacagtc agctgatcgg tcagtttggt gttggtttct actctgcgtt tatcgtggcc
    14341 gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg cgtcttctgg
    14401 gaatcggctg gcgaaggtga atataccgtt gccgacatca ccaaagaaga tcgtggtact
    14461 gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg gcgcgtgcgt
    14521 tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga aaaacgcgaa
    14581 gagaaagacg gcgaaaccgt tatctcctgg gagaaaatta acaaagcgca ggcgctgtgg
    14641 actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa acacatcgcc
    14701 cacgacttta acgatccgct gacctggagc cacaaccgcg ttgaaggtaa gcaggagtac
    14761 accagcctgc tgtacatccc gtcccaggct ccgtgggata tgtggaaccg tgatcataaa
    14821 cacggcctga aactgtatgt tcagcgtgtg ttcatcatgg acgacgcaga acagttcatg
    14881 ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc gctgaacgtt
    14941 tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc gctgaccaag
    15001 cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggaaaaata ccagaccttc
    15061 tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc taaccaggaa
    15121 gcgatcgcca aactgctgcg ttttgcttct actcataccg attcttctgc gcagaccgtg
    15181 tctctggaag actacgtttc ccgcatgaaa gaagggcagg agaaaatcta ctacatcact
    15241 gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg taagaaaggc
    15301 atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta tctgactgag
    15361 ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga aaaactggct
    15421 gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt catcgaccgt
    15481 gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg tctgaccgat
    15541 acgccagcga ttgtctctac tgatgcggac gaaatgagca ctcagatggc gaaactgttc
    15601 gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa cccggatcac
    15661 gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga gtgggtagaa
    15721 ctgctgctgg atcaggcact gctggcagaa cgcggcacgc tggaagatcc gaacctgttt
    15781 attcgtcgta tgaaccagct gctggtttcc tgatgtaatg ccggatgacc ttcgtgtcat
    15841 ccggcatttt tcttttcatt atctgcactt tccgcaaatt atctcgccat taaccgtttc
    15901 agcccctggt gcctttcttg aggcaatcgc ctgatggtgg tatcgtttat cgctttttca
    15961 aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct tggcgctccg
    16021 ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat tccgcaaatc
    16081 tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg taaacaagca
    16141 aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc gctggttaaa
    16201 gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt cccgcgtacc
    16261 attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt tctggaattc
    16321 gacgtaccgg acgaactgat cgttgaccgt atcgtcggtc gccgcgttca cgcgccgtct
    16381 ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggtaaaga cgacgttacc
    16441 ggtgaagaac tgactacccg taaagacgat caggaagaaa ccgtacgtaa acgtctggtt
    16501 gaataccatc agatgacagc accgctgatc ggctactact ccaaagaagc ggaagcgggt
    16561 aataccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg cgctgatctg
    16621 gaaaaaatcc tcggctaatc acgccgcagc agatctgctt tcgggcaggt ctgctcatcc
    16681 acctcaggca atacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta
    16741 tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt atcctgctgg
    16801 caaacctggg tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat
    16861 ttttaagcga cagacgcgtg gttgatacct cacggttgtt atggtggcca ttgctgcgcg
    16921 gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct gtatgcctct gtctggatgg
    16981 aaggtggctc gccgctgatg gtttacagcc gccagcaaca acaggcgctg gcacaacgtt
    17041 taccggagac gcccgtagcg ctgggaatga gctacggctc gccatcactg gaaagcgccg
    17101 tagatgaact cctggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat
    17161 tctcctgttc tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac
    17221 gtagcattcc ggggatctcg tttatacgtg attacgccga taaccacgat tacattaatg
    17281 cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc
    17341 tctcttatca tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt
    17401 gccgcacaac gactcgcgaa ctggcttccg cactggggat ggcaccggaa aaagtgatga
    17461 tgacctttca gtcgcgcttt ggtcgggaac cctggctgat gccttatacc gacgaaacgc
    17521 tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg
    17581 cggattgtct ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg
    17641 ccggcgggaa aaaatatgaa tatattccgg cgcttaatgc cacgccggaa catatcgaaa
    17701 tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt
    17761 cgcgaagagc ctcgtcggcg gttttcatca tccgtgaata atgcaaaaag gcgtgcagcg
    17821 tgcctgggta gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tggtaaagca
    17881 gacggctgtc atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt
    17941 cgcgagtgag atcattatta aacagacagt aatacggcga ctcgcggtcc gcgtagttgc
    18001 ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc
    18061 ccaacagacg acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacac
    18121 ccgcaatttt accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca
    18181 gcatggcacc tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct
    18241 ccgcctgctg gtggaaataa caacaggcag ccacaatttc ctctatcgct tgcggaaaac
    18301 gcgcttcagg tgaaagggtg taatcaatgc caatcaccgt acattggctg tagcttgcca
    18361 gcaggcgcat gatgcgatca tgggtatcga gattgccgag aataaaaccg cctccatgca
    18421 aataaaatag cgttgccggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc
    18481 catattttgt tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga
    18541 atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg
    18601 gcggtaaatc cggctgaaga gtattcacaa cgatcttcat ttcagcagaa ataaggtcca
    18661 gaacaggtaa tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa
    18721 ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc
    18781 gcgctcccgt cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca
    18841 tgaaatttcc cggtaaacgt aaatccaaac attacttccc cgtaaatgca cgcgatccgc
    18901 tgcttcagca attccagcca gaaaacgaaa ccagcgccgc ctgggtagtg ggtatcgatc
    18961 aaacgctggt cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa
    19021 gcgccgggca ttcactggtg attgaggatg atgtagccga agcgctttat caggaactaa
    19081 aacagaaaaa cctgattacc catcagtttg cgggtggcac cattggtaac accatgcata
    19141 actactcggt gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg
    19201 aaattggcag ctatgcctat cgttacctgt gtaacacctc cagccgtacc gatcttaact
    19261 atctacaagg cgtggatggc ccgattggtc gttgctttac gctgattggc gagtccgggg
    19321 aacgtacctt tgctatcagc cctggccaca tgaaccagct gcgggctgaa agtattccgg
    19381 aagatgtgat tgccggagcc tcggctctgg ttctcacctc ttatctggtg cgttgcaagc
    19441 cgggtgaacc catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg
    19501 taccggtggt gctgacgctg ggaactaagt ttgtcattgc cgagaatcca cagtggtggc
    19561 agcaattcct caaagaccac gtctctatcc ttgcgatgaa cgaagatgaa gccgaagcgt
    19621 tgaccggaga aagcgatccg ttgttggcat ctgacaaggc gctggactgg gtagatctgg
    19681 tgctgtgcac cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga
    19741 aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc ggaattcaac cagtatgagt
    19801 ttagccgcgc catgcgccac aaggattgcc agaatccgct gcgcgtctat tcgcacattg
    19861 cgccgtacat gggcgggccg gaaaaaatca tgaacaccaa cggagcaggg gatggcgcac
    19921 tggcagcgtt gctgcatgac attaccgcca acagctacca ccgtagcaac gtaccaaact
    19981 ccagcaaaca taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg
    20041 ctaaccgtgt gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc
    20101 cggagcgtga agacagcctg gaagagtctt actgggatcg ttaagttatc gtcggttcgt
    20161 aggccagata aggcgttcac gccgcatctg gcattttgct ctcaatgcct gatgcgacgc
    20221 tggcgcgtct tatcaggcct acatatccgc gcctattatc cggcaaccac cgtttacccc
    20281 gtcaccactt cacccgccgg tggcgtttcc agcagttcca gcatagtacg ggcaatttca
    20341 cgttcaccca tcactacctg attcgcgcca cgttcggtga tatacgccac ttcatcgtca
    20401 taatgggcgc gggcaataat ctcaatatcc ggatttttcg cgcgggcaga tgccacaatc
    20461 tcgcctgctt cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc
    20521 gccagttgca taatttcttc gttcgccgca ttgcccaata cagcgcggac cccacgctct
    20581 cgcagctcat caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg
    20641 agcaatttct cccccagcag gctgcctaca cgaccgtaac caaccagtag cgcatggttg
    20701 caaatatcca ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc
    20761 agcgtttcgg tcttcgccag atatttctcc agcagcgcaa acaggaccgg gttgagcata
    20821 atcgacaaaa tcgcacctgc cagtaccagg ttctgcccgg cttgcggcaa cagatccagc
    20881 gccatgccca gtcccgccag gataaacgcg aactcaccaa tttgcgccag gctggcggcg
    20941 atagttaatg ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac
    21001 gacttaccaa acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga
    21061 attaacggat caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc
    21121 aatggcagcg tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg
    21181 gcaaagaacg caccgagtgc aaaggagaca tcaaacagct ccaccgcacc aaaggcaatc
    21241 cctaacgcca gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg
    21301 cgtgccataa tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg
    21361 atcaccttgc cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc
    21421 tgttccatca ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct
    21481 tccacaatca accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct
    21541 tcaagtgcgc gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata
    21601 ccggtcatta acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg
    21661 gctatctggg cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc
    21721 gaaaagtgca gacccacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc
    21781 gcaagcttgg tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac
    21841 agatatccca ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg
    21901 agcacaaggc cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct
    21961 tttcctggtg gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta
    22021 tgacaatttc attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta
    22081 gctgcaaaaa aagcacgaat cggaaaacag aaggcggttt aacagaggaa aggcttatgg
    22141 cgactgtaaa acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc
    22201 cggttatcag gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta
    22261 tagactaact cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct
    22321 cccatgccaa aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagc
    22381 agctcctgag cgtaaaccag aatggcagag aatgccgaag cgaggataaa tccaataatc
    22441 accgttaaaa cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca
    22501 acgccgagga tagagcccca aatcacatat ttccgcccaa ttttatcccc tacaggcccg
    22561 ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc
    22621 tggatagata atccgaattt ttgcatcaga taaaaggtgt aatagctgct gatgctcgcc
    22681 atatagaaat atttcgagaa aatgaggatt aacagaatgc tgaccgccag taccactttg
    22741 ttgcgcggca gtggattgat aatcgtcgct ttgggttttc ctttattcat tcggtgctgt
    22801 gccgagtacc aacggctgat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca
    22861 aaccaggcaa cgttgccttt gccataaggc gcgataatca ccgccgccag caagggtccc
    22921 agggaactgc caaagttgcc gccgacctga aagatagatt gtgccaggcc atgccgcccg
    22981 ccggaagcca tacgggccac gcgagaagat tccggatgaa agaccgatga accggtaccg
    23041 accagcgccg ccgccagcag aactgcgcca aaactgcccg ccagcgcaag cagcaccaga
    23101 ccacttaagg taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc
    23161 cagtagccga ccactggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg
    23221 ccaatctgca taaatgtcag agaaaactct gactgaagca gcggataaat cgccagaatc
    23281 agcgattgga tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac
    23341 gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt ttgttcactc
    23401 attgccatag gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat
    23461 tattacccga ctaacatacc tgtatgcgcc gcctgaagga agtctcaacg ctgaatacag
    23521 aatttctaat ctggatgcag atttatcttc accggacgca gacttgtcta tgatgtcgcg
    23581 tcatactatt tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag
    23641 cggggcgtgg cgttagcact gttaaccaca tttacactgg cgagtgaagc tgctctggcg
    23701 tatgagcagg ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat
    23761 ttttggcgca atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtggatggt
    23821 atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggcgacatt
    23881 aacactggcg tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggtatgaat
    23941 ctggtgggct atgacgcgat ggcgatcggt aatcatgaat ttgataatcc gctcaccgta
    24001 ttacgtcagc aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat ctaccagaaa
    24061 agtaccggcg agcgcctgtt taaaccatgg gcgctgttta agcgtcagga tctgaaaatt
    24121 gccgttattg gtctgacgac ggatgacaca gcgaagttag gcaacccaga aaacttcacc
    24181 gatattgagt tccgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag
    24241 acagaaaagc cagacattat tatcgcggcg acccatatgg ggcattacga caatggtgag
    24301 cacggctcta atgcaccggg cgatgtggag atggcacgcg tgctgcctgc cggatcgctg
    24361 gcgatgatcg tcggtggtca ctcgcaagat ccggtctgca tggcggcaga aaacaaaaaa
    24421 caggtcgatt acgtgccggg tacgccatgc aaaccggatc aacaaaacgg catctggatt
    24481 gtgcaggcac atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc
    24541 gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg
    24601 gaagacggga aaagcgagcg cgtgctgtac acccctgaaa tcgctgaaaa ccagcaaatg
    24661 atctcgctgt tatcgccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc
    24721 gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gaccaatatg
    24781 gggcggttaa ttctggcagc acaattggat cgcactggtg ccgactttgc ggtgatgagc
    24841 ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa
    24901 gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat
    24961 tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgccaac
    25021 gttagctttg tggcgaaaga cggcaaactg aacgacctta aaatcaaagg cgaaccggtc
    25081 gatccagcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatgga
    25141 tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg
    25201 ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt
    25261 gaggtgagct ggcagtaatc cgaaagtgcc ggatgttcgc atccggcaca atgcttaatc
    25321 gcggcgggcg atatcagcaa atttggcatc gaggatcttc gccagatcgc ctgccgccag
    25381 ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg
    25441 ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag
    25501 gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct tggcacccag
    25561 cgcttttgct acttttttaa ggtccagttg acctgcgacc ggcgtaacgg ccacggcaag
    25621 gtgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat ccggattcaa
    25681 acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt
    25741 atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat
    25801 tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag
    25861 agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc
    25921 gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag
    25981 cgatattggc cattttttta gcgcaacatt tgcggtaaat tcccttctcc atacaggtgt
    26041 agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag
    26101 gcgagatttc gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc
    26161 gttatttcat tattttgcgg cggcgcattc agccaccagc tcatcatttg ttgcagcaac
    26221 cgtgcattgg tgtgccagtg ggtcagcgta tcgtccagca gtgccagtcc tttgtcaggg
    26281 agctggagca acatggcaat ctggttttca gccccttcca gttcaatcac gggtttatgt
    26341 tgttgcttcg ccgcctgcaa tagctggtaa tcgataccgt attctgcccg cagccccagt
    26401 ttttgcgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg ggtagaaaag
    26461 agtgagggag aaatgcccat ctcctggcta atgtgctgca gtttttggag ttgctcctcg
    26521 ctaatgcgct cttccagcgc ctcgcaggca ggcaaattag caaaaggcgt atcgttggtg
    26581 gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg
    26641 ggcaggggag ccatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga
    26701 ttgccgggaa gggtgatatc gatggcaggc caggtgtaat gattaccgcg cagcgcggcc
    26761 caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct
    26821 agcgcgcggt gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat
    26881 tcagtatgtt cttgtatcgc tattcacagg gaagcaacag ttaaaaacta tgaaacaggc
    26941 aacaagaaaa ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcgc
    27001 tttgaaaatc aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtacact
    27061 gatcaataat aatcgtaaac tcaccactga aatggcattc cgtctggcga aagtttttga
    27121 taccacagtc gatttttggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa
    27181 caacatgcgt acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg
    27241 ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc
    27301 ggataaggcg tttacgccgc atccggcatc cgagcaccat tgcctgatgc gacgcttacg
    27361 cgtcttatca ggcctacaaa cctgtgcctg aaccgtaggc cggataaggc gttcacgccg
    27421 catccggcag tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca tccgcaatga
    27481 cgtacttatt ccttcggttt aaaccgcagc aaccggttgg cgttactcac tacggtaatc
    27541 gacgagagcg ccattgccgc tccggcaact accgggttaa gcagtgttcc agtaaacggc
    27601 cacaaaatac cggcggcgac cggaataccg atactgttgt agataaacgc gccgagcagg
    27661 ttctgtttca tgttgcgcag cgttgcgcgg gaaatagcga gcgcatccgc aacgcccatc
    27721 aggctatggc gcatcagggt aatcgccgcg gtttcaatgg caacatcact gccgcccccc
    27781 atcgcaatgc cgacatccgc ctgggccagc gctggcgcgt cgttaatgcc gtcgcccacc
    27841 attgccacct gacgtccttc actttgcaga cgtttgatcg cttcggcttt accgtccggc
    27901 agcaccccgg ctatcacctc atcaatcccg gcttctttgg cgatcgcatt ggcggtggtt
    27961 gggttatccc cggtcaacat caccagacga tatcccgctt tatgcaggcg ttgcagtgcc
    28021 gccacgctat cactacgcaa tggatcgcgt acagccagca gggctactgc tttcccgtca
    28081 atcgccagca gcacaggcgt tgctccttgc gatgcctgag ctgaaatatc ggcttcaatc
    28141 gctttagtat cgacttgctg atcatttaac aacgcctgat tacccagcaa taacgcatga
    28201 ccttctgctt caccgctcac acccagcccg cgcaatgtgc ggaaaccgtt gacctgcggc
    28261 agctgcatat cgcttgcttt atcgaggatc gcccgtgcca gcggatggct ggaaccttgt
    28321 tccagtgccg ccgccagacg cagcgcctgc gcttcgtcag aatcagcaaa tgttttcact
    28381 gcgacaacct gcggcttccc ttcggtcagc gtcccggttt tatcgaacac gacggtatcg
    28441 agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa ctcagccgcc
    28501 cgcccgacgc cggaaataat cgacatcggc gtcgccagcc ccagcgcaca cggacaggca
    28561 ataatcagca ccgtggtggc aatcaccagg gtatagacaa tctgcggtgc cggaccaaag
    28621 aaataccaga ttgccgcact gacaagcgca ataaccacta ctaccggcac aaatacggct
    28681 gagattttat ccgccagctg accgatttct ggcttgctgc tctgggcctg gcgcaccata
    28741 cgaataatcc ttgatagcgt ggtatggctg ccaaccgcac tggcgcgaaa cagaacgcta
    28801 ccgtcctgta ccactgtccc ggcatggacg ctatcgcctt cgcctttttg ctgcgggatc
    28861 ggttcgcccg tcagcatcgc ttcatccagc cacgcttcgc cctgggtaat ctcgccatct
    28921 accggcacgc ggtcgccggt cgtcaggcgc agtagcatgc ctggctgaac ttccgccagc
    28981 ggcacgcttt tttcaccttc gtcggtgacc aggcgcgccg tcggcggagt taaatcgagt
    29041 aatttctcca gcgcctttga agaacgctga cgtgcgcgtg cttccagcat atggccgaga
    29101 ttgatcagac cgataatcat cgcgctggct tcgtaataaa gatgtcgcgc ttccatcggg
    29161 aaccactgcg gccacaggtt gacgctcatc gaatagagcc acgccacgcc agtacccagc
    29221 gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc actgcggtaa
    29281 aaatggccgc cggcgaaaac catcactgcc agggttatca ggccgataac taaccacaag
    29341 ctgcggttgt cagcggtgac catcatgtta tcgccgatca tcccccagac cattaccggg
    29401 atacccacag ccagtgcaac aattgcctgc cagcggaagc gcttcatcgt agcgacggcg
    29461 gtttcttgct ggcgctcgcg gcgtttcgcg tcatcttcaa tcgcttccgc accgtagccc
    29521 gctttttcca ccgcctgcac taaatcttgt ggggaggcac tgcccatcac cagcgcagta
    29581 cgctccgcca ggtttacccg tgcctgagtg acgcccggta cgctttgcaa cgcattttgt
    29641 acgcgggtga cacagctggc gcagctcatg ccgctcagca gcaactgctg gctgtcatcg
    29701 tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc cgacgggatt
    29761 gatgactccg ccagcggttt agcctttggg tggcttacag atgcgtcata acccgcttgt
    29821 ttgatggttt cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc ttcagtgata
    29881 gacacatccg cttgttccac atccgggcgc tgttcgagac tttctttcac gcgtttaacg
    29941 cagtgaccgc aggacaggcc gtccagggtc aggtcgatag tttgtgacat aaaacactcc
    30001 tttaagacag ttttgactgg ctgtgataaa ggttaaacct tccagcaagg ggaaggtcaa
    30061 gaaattaata acccaggcga gtaaaagtcc gtaaagatta aaaaatcggc tcgatttgca
    30121 tcaggattag acatttatct ctttgttttc ctgtagttaa gttgcgggtg ctaagttaaa
    30181 tcaggatgcc tgaaaatcgg caccggggtg aggaattacc tcccgcatct ataaaaagga
    30241 gttaacaaaa gatgttagat gcaaacaaat tacagcaggc agtggatcag gcttacaccc
    30301 aatttcactc acttaacggc ggacaaaatg ccgattacat tccctttctg gcgaatgtgc
    30361 ctggtcaact ggcagcagtg gctatcgtga ccagcgatgg caacgtctat agtgcgggtg
    30421 acagtgatta ccgctttgcg ctggaatcca tctcgaaagt ctgtacgtta gcccttgcgt
    30481 tagaagatgt cggcccgcag gcggtacagg acaaaattgg cgctgacccg accggattgc
    30541 cctttaactc agttatcgcc ttagagttgc atggcggcaa accgctgtcg ccactggtaa
    30601 atgctggcgc tattgccacc accagcctga ttaacgctga aaatgccgaa caacgctggc
    30661 agcgaatttt acatatccaa cagcaactgg ctggtgagct ggtagcgctc tctgacgaag
    30721 tcaaccagtc ggaacaaaca accaacttcc ataaccgggc cattgcctgg ctgctgtact
    30781 ccgccggata tctctattgt gatgcaatgg aagcatgtga cgtgtacacc cgtcagtgct
    30841 ccacgctcat caatactgtt gaactggcaa cgcttggcgc gacgctggcg gcgggtggtg
    30901 tgaatccgtt gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac attctggccg
    30961 aaatgatgat ggaagggctg tatggtcgct ccggtgactg ggcgtatcgt gttggtttac
    31021 cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt ccctggagtg atgggaattg
    31081 ccgcgttctc accaccgctg gacgaagaag gcaacagtgt tcgcggtcaa aaaatggtgg
    31141 catcggtcgc taagcaactc ggctataacg tgtttaaggg ctgatcatga tgaacacgga
    31201 aggtaataac ggtaacaaac ccctcggtct atggaacgtc gtttccatcg gcattggggc
    31261 aatggtgggg gcggggatct tcgcgctgct ggggcaggct gcgttgctaa tggaagcctc
    31321 gacctgggtc gcctttgctt ttggcggtat tgtggcgatg ttttccggtt atgcctatgc
    31381 gcgtctgggg gcgagctatc ccagcaatgg cggcattatc gacttctttc gtcgcggatt
    31441 aggcaacggc gtcttttcgc tggcgctctc gttactgtac ctgttgacgc tggcggtgag
    31501 catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg cagtttttgc atgaaggcag
    31561 ccaggaggag caccttattt tgctctacgc gttggggatc attgcggtga tgacgctttt
    31621 caactcctta agcaaccatg cggtagggcg gctggaggtg atcctcgtcg gcattaaaat
    31681 gatgatcctg ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg cgcatatttc
    31741 cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt atcgggataa ccttccttgc
    31801 ctatgcgggc tttggcatga tggcgaacgc ggcggataaa gtgaaagatc cgcaggtcat
    31861 tatgccacgg gcgtttctgg tggcaattgg cgttaccacg ttgctttata tctcgctggc
    31921 gctggttttg cttagcgatg tatcggcatt agagttagaa aaatatgccg ataccgccgt
    31981 agcgcaggca gcttctccgc tgctcgggca tgtgggttat gtgatcgtcg tcatcggcgc
    32041 tttactggcg acggcttcag ccatcaacgc gaacctgttc gccgtgttta acatcatgga
    32101 caacatgggc agcgaacgcg aactgccgaa gctaatgaat aaatccctgt ggcggcagag
    32161 tacctggggc aacatcattg tcgtggtgtt gattatgctg atgacggcgg cactaaattt
    32221 aggctcactc gccagcgttg ccagcgccac ctttttgatt tgctatctgg cggtgtttgt
    32281 ggtggcaatc cgcctgcgtc atgatattca cgcctcgttg ccgattctta tcgtaggtac
    32341 gttggtgatg ttgttggtga tcgttggctt tatctacagc ctgtggtccc agggcagccg
    32401 tgcgttgata tggattattg gtgcaatctt actcagcctt attgtggcaa tggtcatgaa
    32461 gcgcaataaa accgtataac acctctctgc gcgcagtact gcctgtactg cgcctttgtc
    32521 tttaattcaa attacctttc ttattccttt aataaataag tcacaaaaaa atgatatata
    32581 cgcgataaat ctgtgatgct aatcacttat taccgtggat tattcttaat gtgtatgatg
    32641 gtaatctaaa agtcgtagtg tattctaaat aaataacgta taagcttttt agctgcattt
    32701 ctttgccaat atcaatgatc gacaaaagag atgcacaagt tattaccctt cttaataaaa
    32761 tgagactggt tataaaaacc agttaaaata atgacctgga aggaagtgac ttatctatgg
    32821 cgcgattcca gtttaaaagc cgtaaaaata atgggtttat ttttttaata agttttatgg
    32881 tgatgggaga agctacaatt gctgcaccgc tgccacaatg ggcgaacgcc ccagcggtaa
    32941 cgccagttgc ccaattatcc ttacaggaaa gtatattacg cgcctttgcg cgaaatcccg
    33001 acgtcactca acaggcggcg cagataggca ttggcgaagc gcaaattgat gaagccaaaa
    33061 gtgcctggta tccgcacgtt ggattaaccg gcaacgcggg gccgtcccga caaacggact
    33121 ccagcggcag gcttgataac aacgtttcgt atggcataac cctgacacag ctggtgtatg
    33181 actttggtaa aactaacaac gatatcaatc tgcaaactgc cgcccgtgac agctaccgct
    33241 ttaaattgat ggcaacctta accgatgttg cagagaaaac ggcgactgcc tatatggaag
    33301 tcagtcgtta tcaggctttg tgcgatgcgg ctcaacgcaa tattcactcg ctggaaaacg
    33361 tctacaacat ggcggcattg cgcgctaacg ctggcctgaa ctcatcgtcg gatgaattac
    33421 aggcccagac gcgtattgcc ggaatgcgct caacgctgga gcaatatcag gcgcagatgg
    33481 tgagcgccaa agcgcaactg gcggtgctca ctggcgtaca gccggaggcg atagccgcgc
    33541 caccggctga attagccgag cagccggtat cgctaaaaaa tattgattac cagtccatcc
    33601 cgctggtgct ggcggcagaa aacttacgcc agtcagcaca gtacggcgtg gaaaaaacga
    33661 aagcgcaata ctggccaacg ctcagtattc aggggggtaa aacgcgctac cagaccagcg
    33721 accgctcgta ttgggatgat cagctacaac tgaacgttaa cgcgccgctg tatcagggcg
    33781 gcgcggtttc tgcccaggtg caacaggccg aagggcaaca aaaaatctct gcctcgcagg
    33841 tcgaacaggc aaaactggat gtgctgcaac gagcgtctgt ggcatatgcg aactggaccg
    33901 gcgcacgggg tcgtgaagaa gccggtttag cgcaatccga aagtgcgcac aaaacgcgag
    33961 atgtgtacca aaatgaatat aaattgggta aacgcagtct gaatgatcta ttaaccgtcg
    34021 agcaagatgt ctttcaggcg caatcggctg aaataaatgc caattatgat ggctgggtcg
    34081 ccgccgtaaa ttatgccgct gcggtgaata accttattcc attagcggga attaaacagg
    34141 gcttatacaa cgacttaccc gacttgaaat aatcctgcca ttattactag tgggccgttg
    34201 ttaagtgatt acctttgtct tatttagata aatggactcg ctaaggatgt gcgccattta
    34261 tcaggaatgc cattatgagc ctgattattg atgttatttc gcgtaaaaca tccgtcaaac
    34321 aaacgctgat taatcctggc gacgtcacgg ttgttattta tgagccttcc gtggtgcagg
    34381 ttcatgctca ggcctctgcc gttgcgcgtt acgtccgtga aggaaatgac ctgctgatct
    34441 atatgcagga cggcacggtg atccgctgca acggttattt cctgcaagcg gcgaatacag
    34501 ctgaacaatc ggaactggtg tttgccgatg gtcaacagct aacccatatc acctttgccg
    34561 atactgctgc gggtggatta gcccccgtag aactgactgc ccagaccact gcgattgaaa
    34621 gcattgcgcc atttcttgat accgttgctc agaccagcgc tttcccgtgg ggttggctgg
    34681 cgggggcggc ggtaggtggt ggcgcgcttg gtgcactgct ggcaagcggt ggcgatggcg
    34741 actcgaaaac agaagtgatt aataacccta cgccacccgc tgagcctggc aacgccacac
    34801 catcattttt agttaccgat aatcagggcg atcagcgcgg cattctcgcc accaatgaca
    34861 tcaccgatga caccacgcca acctttagcg gcagcgggca ggcgggggcg actattcaga
    34921 ttaaagacag taacggcaat actattgcca gtactcaggt agacaacaac ggtcactgga
    34981 gtgtctcgtt acccacgcaa agtgcaggtg aacatacctg gtcagtggtg caaattgtcg
    35041 gcagtaccat cactgacgcc ggttcgataa cgttaaccat cgacaatagt caggccagcg
    35101 tgcaggttgc caccaccgca ggcgataaca ttattaacgc cagcgaacag gccgccgggt
    35161 ttacgctttc tggcaccagt agccatctgg cgcagggaac agaactcacc gtcacactta
    35221 acggcaaaac ctacaccaca actgtagggg ctaacggtgc ctggagcgtg caggtgccga
    35281 ccgccgatgc acaggcgtta ggcgaaggga atcaggcggt actggtcagt gggaaagaca
    35341 ccacaggcaa tacggtcacc ggcgcgcagc tgctaacggt cgatacccaa ccgccaacgc
    35401 ttgccatcaa caccatcgcc caggacaaca tcatcagtgc tgcggaacat aacgtcgcgc
    35461 tggtactgag cggcacgtcg aatgcagaag cggggcaaac cgtaacactg accgtcaacg
    35521 ggaaaagcca tacagcaacc gtcggtagcg acggaacctg gcaagtgacg ctgcctgcca
    35581 cggaagtcca ggcactggcg gagggtaatt acgctgtcaa tgccagtgtc agcgatcggg
    35641 cagggaacac caccagccac agcgcgaatt tcacggtaga cacctcagca cccgtggtca
    35701 gtgttaatac cgtggcgggc gacgatattc ttaataatgc cgagcaggcc gtcgcgcaga
    35761 tcatctccgg acaagttagc ggtgcttctc ctggcgatac ggtaacggtg aaattgggca
    35821 ctcatgtcct gacgggcatc gtgctggcag atggcagctg gaatgtggcg ttggacccgg
    35881 cggtaacccg cacgctggat cgcggagcca atacgatttt cgtcaccgtg acagatgctg
    35941 caggaaatac tggcgcggcg tctcgagcaa tcacgctggt cggtgtttct ccgttgatca
    36001 ccattaacac cgtctccggc gatgacatta tcagtggcgc agaaaaaggt gcgccactga
    36061 cccttaccgg tagcactcaa caggctgaga caggacaaac cgtcacagta accctggctg
    36121 gacagagttt taccactacc gtgcaggccg atggctcctg gagtctgacg gtacctgccg
    36181 ccgcgatggg aaatctgcct gacggcgagg tggcgattac cgcttctgtg acggatctca
    36241 gcggcaatac cggcaacacc tcccgcacca ttaccgtcga cagccaggcc ccggcctaaa
    36301 gcattgatcc actgaccgct gataacatca ttaacgccgc cgaaagcggg caggatctgc
    36361 ccatcaccgg caccaccgac gcccagccag ggcagacggt aacggtgacg cttaatgggc
    36421 agacgtatca gggcgtcgtg cagccagacg gcacctggag cgtgaccgtt cccgccgcca
    36481 acgttgacgc gctggctgac ggtaacgcca cggtcaccgc cagcgtgaac gatgtcgccg
    36541 gtaacccgac cagcgtctca cgcgtggcgc tggtcgatgc cacgccgccg gtggtgacca
    36601 ttaatccggt ggcgaccgat aatgtcatta acacgccgga acatatccag gcgcaaatta
    36661 tcagcggcac ggtcactggc gctcaggcgg gcgatatcgt caccgtgacg ctgaataatg
    36721 tggattacac cacggtggtg gatggttccg gcaactggag tctgggcgtt ccggcctcgg
    36781 tggtcagtgg gctggcggac ggcagttatc ctgtcagcgt ctcggtaacc gacaaagccg
    36841 gaaacacggg cagccagtca ttgaccgtca cggtcaatac cgccgcgccc cttatcggca
    36901 ttaacagcat tgcgggcgat gatgtgatta acgccagcga aaaaggggcc gatctccaga
    36961 ttaccggcac cagcgatcag cctgttaaca ccgccatcac cgtgacgctg aacgggcaaa
    37021 attacaccac cacgaccgac gcctccggca actggagcgt caccgttccg gcatcggcgg
    37081 taaccgcact gggccaggcc aactacacgg taacggcggc ggtgaccagc agtatcggca
    37141 atagcaccac cgccagccat aacgtgctgg tcgacagcgc gctgcccggc gtgaccatta
    37201 acccggtggc aaccgacgat attattaacg ccgccgaagc gggcgtggcg caaaccatca
    37261 gcgggcaggt gacaggagcc gccgttggcg acgcggtaac cgttacgctg ggtggcaata
    37321 actacaccac caccgtgcag gccggcttaa gctggagcgt ggacgttccg gcggacgcta
    37381 ttcaggcgct gggtaatggc gatttaacgg ttaatgcctc ggtcaccaat caaaacggca
    37441 acaccggcag cggcacgcgg gatatcacca tcgacgccaa tctgcccggc ctgcgggtcg
    37501 atacggtggc gggcgatgat gtggtcaata tcatcgagca cgggcaggcg ctggtggtca
    37561 ccggcagcag cttggggctt gctgaaggca cgccgcttac cgtcacgatt aacgatgtgg
    37621 aatacaccac tgcggtgcag gccgatggca gctggagcgt gggcatcacg gcggcgcagg
    37681 ttagcgcctg gcctgcgggg gcggttaata ttgccgtatc cggggaaagt agcgccggaa
    37741 atccgataag cattacgcat ccggtaacgg tggatctcac tccggcggcg atcaccatca
    37801 acaccatcgc cacggatgac gtgattaacg ctgcagaaaa aggggctaat ttaactcttt
    37861 ccggcaccac cactaacgta gaagcagggc aaaccgtcac tgtcaccttt ggcggtaaaa
    37921 attacaccgc cagcgtggcg ggcgatggta gctggactgc caccgttccc gccgccgatc
    37981 tggcagcatt accagagggc agcgcctccg cgcaggccag cgtcagcaat atcaacggca
    38041 atagcgcctc ggcggtgcac agctacagcg tcgacagcag cgcgccaacc atcattatca
    38101 ataccgtcgc cagcgacaat atcgtcaacg ccagcgaagc cgatgcgggt gtgacggtga
    38161 gcggcagcac caccgctgaa gcggggcaga ttgttacggt aacgctcaat agcccgaccg
    38221 tgcagacgta tcaggcaacg gtgcaggcgg acggcagctg gagcatcaat attccggcgg
    38281 cagatcttga ggcattgacc gatggcagcc acaccctgac cgccacggtc aatgacaaag
    38341 cgggcaaccc ggcgagcacc acgcataatc tggcggtgga tctcaccgtt ccggtactca
    38401 ccatcaacac cattgcgggc gatgacatta taaacgccac cgaacacggg caggcgctgg
    38461 tgatttccgg ttccagcact ggcggagaag cgggagatgt cgtcaccgtc acgctaaaca
    38521 gtaaaaccta caccacgacg ctggatgcct ccggcaactg gagcgtcggc gttccggcgg
    38581 cggatgtcac ggcgctgggt agcggcccgc aaaccgtgac tgcgaccgtt accgatgccg
    38641 ccggtaacag cgatagtgag acgcacacgg tcaccgttaa ccttaccgcg ccgaccattg
    38701 gcattaacac catcgccacc gatgacgtga ttaacgccac cgaaaaaggc gcggatctgc
    38761 aaatttccgg caccagtaat cagccagcag gcaccacaat tacgctgacg ctgaacgggc
    38821 aaaattacac tgccaccaca gatgcttccg gcaactggag caccacagtg ccagcttccg
    38881 cagtcggtgc gctgggcgaa gccagctaca cggtgacggc gaacgtcacc gacagcgcgg
    38941 gcaacagcaa ctccgccagc cataacgtgc aggtgaatac cgcgctgcct ggcgtcaccc
    39001 ttaatccggt agcgagcgac gatattatca acgctgccga atcgggcgtg gcgcagacca
    39061 tcagcggcca ggtcacgggc gcggcggcgg gcgatacggt taccgtaacg cttggcggga
    39121 aaacctacac cgctaccgtg caggggaatt taagctggag cgtggacgtt ccggcggcgg
    39181 atattcaggc catcggcaat ggcgagctga cggttaacgc ctgggtaacc aacggcgttg
    39241 gcaataccgg cagcggttcg cgcgatatta ctatcgacgc caacctgcct ggcctgcgcg
    39301 tcgataccgt ggcgggcgat gatgtggtta acagcatcga acacggtcag gcactggtga
    39361 taactggtag cagcagcggg ctggcggcgg gcgctgcgct gacggtggtg atcaataacg
    39421 tcacctatgg cgcgacagta ttagccgatg gcacatggag ccttggcgtt ccggcggcaa
    39481 atgtaggcaa ctggcctgcg ggtacggtga atatcacggt ttcaggtgct actagcgcag
    39541 gaaacccggt caccatcacc catccagtca ccgtcgatct ggcagctgtg gcgatctcca
    39601 ttaacaccgt ttccggcgac gatgtgatta acgccgccga aaaaggggca gatttaaccc
    39661 tttccggcag cacctccggc gtggagccag ggcaaacggt caccgtcacc tttgacggca
    39721 aaacctacac cgccaccgta gcaggcgatg gtagctggac aaccaccgtc cctgccgccg
    39781 atctcgccgc attacgcgac ggcgacgcca cggtacaggc cagcgtcagc actattaacg
    39841 gcaacacggc ttcggcaacc cacgtctaca gcgtcgatgc cacgaccccg acgcttacca
    39901 ttaacaccat cgccaccgac gatattctga acgccgccga ggcgggtaat ccgctaacca
    39961 tcagcggcag cagtaccgcc gaagcggggc agacggtaac cgtcacgctt aatggcgtga
    40021 cttacaccgg caccgttcag gcggacggta gctggagcgt cagcgtgccg acggcggatc
    40081 tcagcaatct gaccgccagc cagtacaccg tcagcgcctc ggtaaacgat aaagcgggta
    40141 acccggtgtc cgccactcac gggctggcgg tggatctcac cgtgccggtg ctgaccatta
    40201 acaccgtctc gggcgatgac attattaacg ctactgaaca cgggcaggcg ctggtgatct
    40261 ccggttccag tactggcggc gaagcgggcg atgtcatcac cgtcacgtta aacagtaaaa
    40321 cctacaccac cacgctggac gcttccggca actggagcgt cggcgttccg ctgtctgatg
    40381 tcaccgcgct tggcagcggc ccgcaaaaca tcacggccac aattaccgac gcggcaggca
    40441 acagcgatga cgccagccgc acggtcaccg tgaatctcgc cgcgccaacc attggcatca
    40501 acaccatcgc caccgatgac gtgattaacg ccacggaaaa aggcgcggat ctgcaaatta
    40561 ccggtaccag taatcagcca gcgggcacca cgataacggt gacgctgaac gggcaaaatt
    40621 acaccgccac cacagatgct tccggcaact ggagcgccac ggtgccagcc tcggcagtta
    40681 gcgcattggg tgaagctaac tacacggtaa cggcaaacgt caccgacaag gcaggcaaca
    40741 gtaattccgc cagtcataat gtgctggtca acagcgcctt gcctgccgtt accattaacg
    40801 cggtggcgac tgacgatatt atcaatgccg acgagtcgga caatgcgcaa accatcagcg
    40861 gccaggtgac gggggccgcg gcgggagata ccgttaccgt gacgctgggc ggcaacacct
    40921 acaccgccac ggtgcaggct aatttaagct ggagtatcag cgttccggcg gcggatattc
    40981 aggcgctggg taacggcgac ctgacaatta atgccttggt caccaatggc gtgggcaaca
    41041 ccggcagcgg ctcgcgcgat atcacgatcg acgccaatct gcctggcctg cgggtcgata
    41101 ccgtggcggg cgatgatgtg attaacagca ttgagcacaa tcaggcgctg gtgatcaccg
    41161 gcagtagcac cgggctgacg gcgggaacgg cgttaacggt cgagattaac aacgttactt
    41221 acgctgccac ggtgttagcc gacggcacgt ggagcctcgg tgttccggcg gtagacgtca
    41281 gcaactggcc agcgggtacg gtgaatatca cggtaagcgg caccaacagc gccggaacaa
    41341 cctccaccat tacccatccg gtcaccgtcg atctggcggc ggttgccatc accattaaca
    41401 ccctttccgg cgatgatgtg atcaacgccg tcgaaaaagg cgaaacgctg gtggtgagcg
    41461 gcagcaccag cggtgtggaa gccgggcaga cggtgaccgt cacctttggt ggcaaaaatt
    41521 acaccactac cgtggaagcc aacggcagct ggacggtgaa tgtgccgcct gccgatctcg
    41581 ctgcgctgcc ggatggcgcg ggcaacgtgc aggcgagcgt cagcaacatc aacggcaata
    41641 acgcccaggc ggatcgcgcg tacagcgttg atgccaccgc gccacttgtg accatcaaca
    41701 ccatcgccag cgacgatact cttaacgtga gcgaagcggg cgcggggata accatcagcg
    41761 gtaccaccac ggcgcaggcc gggcagacgc tcaccgtcac gctcaataac aacacgtacc
    41821 agaccaccgt tcaggcggat ggcacctgga gcgtgaatgt tccggcagcg gatttaagcg
    41881 gattaaccgc cagcagttac accgtgaccg ccacggtgag cgacaaagcg ggtaacccgg
    41941 caagcgccga ccacgcgctg gcggtggacg ttaccgcacc ggatctcacc attaataccg
    42001 tcgcgggcga tgacattatc aacgccatcg aacacggtca ggcactggtc gtcagcggaa
    42061 ccagtacggg cgcggcggcg ggcgatgtgg tcacggttaa cctgaacggt aaaaactaca
    42121 ccaccacgct ggacgcctcc ggtaactgga gcgtgggcat tccggcggcg gatgtcacgg
    42181 cgctggcaac cggcagccag acgatcaccg ccagcctgag cgatcgcgcg ggcaacagcg
    42241 acagcactac ccataatgtg accgttgatc tcagcggccc gacgctgacc atcagtatcg
    42301 tctccggtga tgacattatt aataatgcgg agaaaaccca ggatctaatc atcagcggcg
    42361 gctccagcgg cctggcgacc ggcactacgg tcaccgtgat gttgaacggc ctggcgtaca
    42421 gcgcaactac cgacggttct ggcaactgga gcgttactgt gcctgcttcg gcggtcggcg
    42481 cactgggcga ggcggtgtac cagattagcg ccagcgctac cgacagcgcg ggcaacagcg
    42541 gcatcaccac ccatacggtg aatgttgaat cgctcctgcc aggggtgatc attaataccg
    42601 tggcgggcga tgacattatc aacgccgctg aaattggtgt taaccagacc atcagcggac
    42661 aggttacggg gacggcagca gccggagata cggtcactgt cacgctcggc ggtaatcaat
    42721 acgtcgccac cgtacagcca gatttaagct ggagcgtcag cgtaccggcg aacgttttgc
    42781 aggcgctggg caacggtgag ttgaccatca gcgcctcggt gaccaattcc gcaaataata
    42841 ctggcaccac aacgcacgac atcgtgatag atgccaacct gccaggcctg cgggtcgata
    42901 ccgtggcagg cgatgatgtg attaacagca tcgagcacac tcaggcgttg gtgatcaccg
    42961 gtagcagtag cggactggcg gcgggcgcgg cgttgacggt ggttatcaat agcgtcacct
    43021 acggcgcaac ggttttagcg gatggtagct ggagcgttgg tgttccggcg gcagatgtca
    43081 caaactggcc tgcggggacg gtcaatattg ccgtctctgg caccaacacc gccggaacga
    43141 caaccagtat tacccatccg gtgaccgtcg atctcgcagc cgtggcgatc accatcaacc
    43201 ccctttccac tgacgatgtg attaacgccg ctgagaaagg ctctgatttg cagctctccg
    43261 gcaccacctc cgatgtggaa gcggggcaaa ccatcaccgt tatcttcggc ggcaaaagct
    43321 acaccaccac ggttgccgcg gataatacct gggggctgac aatcccagcc gccgatctcg
    43381 ccgctttacc ggatggcgca gcaaacgtcc aggccagtgt cagcagcgtc gcaggtaaca
    43441 gcgcccaggc aacgcatgtt tacagcgttg atgccaccgc gccctccgtc accatcaaca
    43501 ccatcgccgc agacgatatt cttaacgccg ctgaagcggg atcggcgctg atcatcagcg
    43561 gcaccagcac gacagaagcc gggcagacgg taaccgtgac gcttaacggc gttaattaca
    43621 gcggcaacgt gcaggctgac gggagctgga gcgtcagcgt accaactggt gatttagcta
    43681 accttaccgc cagctcgtat accgttaacg cctcggtcag cgacaaagcc ggaaatccgg
    43741 cttcggcaac gcataatctg acggtggacc ttgccgctcc ggtcgtcacc atcaacacgg
    43801 tggcgggcga tgacatcatc aacgccacgg aacacggaca ggcgcagatc atcagcggct
    43861 cggcaacggg cgcgactacc ggtaatacgg tttccgtgac gattggcacg accacctata
    43921 ccaccgtgct ggacgccaac ggcaactgga gcatcggcgt gcctgccagc gtgatttccg
    43981 cactggcgca gggcgatgtg aacattagcg ccacggttac cgactccgca ggcaacagcg
    44041 gcacagcctc gcacgttgtc agcgtggcgc tcggcgctcc ggtgctcgcc attaacacca
    44101 ttgccgtcga tgacatcatc aacgccacgg agaaaggcgc ggatctggcg atttccggca
    44161 ccagcgacca gcctgcgggc acgcagatta ccgttacgct caacgggcaa aattacacca
    44221 ccacagccga tgcttccggg aactggagcg tgaccgttcc ggcgtctgcg gttggtacgc
    44281 ttggtgaagc gacttatacc gtgaccgcct ccgccactga cgccgatggc aacagcggtt
    44341 ccgccagcca taacgtacag gttaataccg cgctgccggg cgtcaccatt aacgtggtgg
    44401 caacggacga tattattaac gccgccgaag cgggcgtgga ccagaccatc agcgggcagg
    44461 tgacgggtgc ggcggcaggc gacacggtga ccgtcacgct cggcggggcg acttacactg
    44521 ccacggtgca ggcgaattta agctggagcg tcgatgttcc ggcctccgcg ttacaggcgt
    44581 tgggcaacgg tgagctgacc atttcggctt cggtaacgaa cagcgtaggc aataccggca
    44641 acggcacgcg tgaaatcacc atcgacgcga atctgccagg tctgcgggtc gataccgtgg
    44701 cgggcgatga tgtggtcaat atcatcgagc acgggcaggc gctggtgatt accggcagca
    44761 gctccgatct ggcagcgggc agcaacgtca cgctgaccat aaacgggcaa acgtatgttg
    44821 cggcggtgct ggcggatggc tcatggagcg tcggtgttcc ggcggcagat gtcagcgcct
    44881 ggcctgcggg gacggtcacg attacagcca gcggcaacac caccgccgga aacccggtga
    44941 gcgttacgca tccggtgact gtcgatctca cggcggtggc ggtcagcatc aacgccatta
    45001 ccgtcgatga tgtgattaac gccgctgaaa aaggcgcggc gttaacgctc tccggcagca
    45061 cctctggcgt cgaagccggg caaacggtca ccgtgaactt tggcggaaaa acctacactg
    45121 caagcgtggc gacgaacggt tcctggagca ccacggttcc ggcggcagat atggcggccc
    45181 tgcgtgatgg cgatgccagc gcacaggcca gcgtcagcaa tgttaacggc aacagcgcca
    45241 ccactactca cgcttacagc gtcgatgcca gcgcgccaac ggtgaccatt aacaccattg
    45301 cgggcgacga cattcttaac gccgccgaag ccggagcggc gctgaccatc actggcagca
    45361 gcacggcgga agcggggcag acggtgaccg tcaccctcaa tggcgaaaac tatactggca
    45421 cggttcaaac ggacggtagc tggagcgtca gtgtgccgca agccgacgta agcgccctga
    45481 ccgccagcaa ctacaccgtg agcgcggcgg tgaacgacaa agccggaaac ccggcctcgg
    45541 ttaaccacaa cctgacggtg gatacttctg ttccggtcgt caccatcaac acggtggcag
    45601 gcgatgatgt gatcaacgcg acggaacacg cccaggcaca gatcatcagc ggatccgcca
    45661 cgggtgcggc aaccggtagc accgtgacgg tgactatcgg cacaaatact ttcaccacgg
    45721 tgctggatgc cagcggcaac tggagcgtcg gcgttccggc aagcgtcgtc tcggcactgg
    45781 cgaacggcac ggtgaccatt aacgccagcg tcaccgatgt cgccggtaac agcggcagcg
    45841 ccacgcatca ggtgacggtc aataccgggc tgccgaccat tacttttaac gccatcagcg
    45901 gcgataacgt cctgaacgct gatgaaaaag gccagccgtt gaccatcagc ggcggcagta
    45961 cggggctggc gacgggcgcg caggtcaccg tcacgctcaa cggtcacaac tacagcgcca
    46021 ccaccgacgc gtcgggcaac tggaccttaa ccgtgccggt gagcgatctg gcggcattag
    46081 gtcaggccaa ctatacggtc agcgccagcg ccaccagtgc agcaggcaac accgccagca
    46141 gccaggcgaa tttactggtc gacagcggcc tgccggacgt caccatcaac accgtggcag
    46201 gcgacgatat tatcaacgcc gccgaagcgg gggccgatca aaccatcagc ggggtggtga
    46261 ctcgcgccgc cgctggcgat acggtcaccg tgacgctggg cgggaacact tacaccgcta
    46321 cggtacagag caacttaagc tggagcgtca gcgttccgac agccgatctc caggcgttgg
    46381 gcaatggtga tttgaccatt accgcctcgg tcaccaacgc taatggcaac accgggagcg
    46441 gcacgcggga tatcaccatt gatgccaacc tgccggggct gcgcgtagat accgtggcgg
    46501 gcgatgatat cgtcaacagc atcgagcacg ggcaggcgct ggtgatcacc ggcggcagta
    46561 gcggcctgaa tgcaggtgct gtgctgacgg ttaccatcaa cagtgtggcg tattccgcca
    46621 cggtgcaggc ggacggaagc tggagcgttg gcattccggc ggcaaacgtc agcgcctggc
    46681 ctgcggggcc gttaaccgtt gaggtagccg ggcaaagcag cgccgataac ccagtcagcg
    46741 tcagccatcc gttcaccgtc gatttaacgg cggtggcaat cagcatcaat acggtcgcca
    46801 gcgacgacgt gattaacgcc gccgagaaag gcaccaatct gaccctttcc ggcagcacca
    46861 gcgggattga aagcgggcaa accgtaaccg tcacctttgg cggcaaaacc tacaccgcaa
    46921 gcgtcgccgc caacggaagc tggagcaaaa cggttccggt ggcagatctg gcaactctgc
    46981 cagacggcgc ggcgaacgtg caggccagcg ttagcagcgc gagcggtaac agtgcctcgg
    47041 cgacccatgc gtatagcgtt gacgccagcg cgccgacgct caccattaac accatcgcca
    47101 gcgacgatat ccttaacgcc acggaggccg gaagcccgct caccatcagc ggctccagca
    47161 ccgccgaaac cgggcagacg gtcaccgtca cccttaacgg cgcaacctac accggcactg
    47221 tacaggcgga cggcggctgg agcgtcagcg ttcctacttc agccctgggc gcgctcaacg
    47281 caagcaatta caccgtcagc gccacggtca acgacaaagc gggcaacccc ggcagcgcca
    47341 gccacaatct ggcggtagat accaccgcgc cggttctcac cattaacacc gtggcgggcg
    47401 atgacatcat caacgacgcc gaacacgccc aggcgctggt gatctccggt accagcacgg
    47461 gcggggaagc gggcgatgtg gtgagcgttg tgctcaatgg caaaacctac accaccacgc
    47521 tggatgcctc cggtaactgg agcgtcggcg taccggcggc ggatgttgcg gcgctgggta
    47581 gcggcgcaca gaccatcacc gccagcgtca gcgatcgggc aggcaacagc gacgacgcca
    47641 gccgcaccgt gaccgtcagc ctcagcgcgc cagtgattag catcaacacc atcgcgggcg
    47701 atgatgtgat caacgcgacg gaaaaaggat ctgatctggc gctttctggc accagcgatc
    47761 agcctgcggg tacggcgatc accgtcaccc tgaacggaca aaactacagc gccaccacgg
    47821 atgcctccgg caactggagt gttaccgtgc ctgcctcggc ggtcagtgcg ctgggcgaag
    47881 cgacctacag cgtgacggcg agcgtcacca atgctcaggg taacagcagc accgccagcc
    47941 ataacgtgca ggttaatacc gcgctgccgg gcatcaccat taatccggtg gcgacggacg
    48001 atattatcaa cgcttcagag gcaggcagcg cgcaaactat cagcggccag gtaaccggtg
    48061 cggcggcggg cagcaccgtc accgttgaac tgggcggtaa aacttacacc gccaccgtgc
    48121 agccagattt aagctggaat gtaagcgtac ctgccgccga ctggcaggcg ctgggtaacg
    48181 gtgagttaac ggttaatgcc tcggtgacca acgccgttgg caataccggc agcggcacgc
    48241 gcgatatcac catcgatgcc agtctgcccg gcctgcgggt ggataccgtc gcgggagatg
    48301 atgtggtcaa tatcatcgaa cacagtcagg cgcaggtgat caccggcagc agctccggct
    48361 ttgccgcagg cacggcgttg acggtggtga ttaacaacca aacttacgcc gccacggtgc
    48421 tggtgaatgg tacctggagc gtcggcgttc cggcggcgga tgtcagtaac tggcctgcgg
    48481 gaacgctgaa tattaccgtt agcggcgcga acagtgccgg aacgcaaacc agcattaccc
    48541 atccgctgac tgttgatctc actaccgttg ccatcagcat taacgctatt acccctgatg
    48601 atgtgattaa cgccgccgaa aaaggcgcgg cattaacgct ctccggcagc acgtccggcg
    48661 tcgaagcggg gcaagccgtc accgtcacct ttggcggtaa aacctacacc accacggtgg
    48721 cggcaaacgg tagctggagc accactgttc cggctgcaga tttggcggcg ctgcgtgacg
    48781 gcgatgccag cgcccaggtg cgggtgacta acgtcaacgg caacagcgcc acagccacgc
    48841 atgaatacag cgtcgatagc gccgcgccaa ctgtgaccat caacaccatt gccagcgata
    48901 acatcatcaa cgccagcgaa gcggcggcgg gcgtaacggt gtccggcacc agcacggcgc
    48961 aaaccgggca gacgctcacc gtcacgctta acggcactaa ctaccagacc acggtgcagg
    49021 cagacggcag ttggagctta acgctgcccg ccagcgactt aaccgcactg gccaataacg
    49081 gctacaccct gaccgctacg gtcagcgatc tggcgggtaa ccctggcagc gccagcaaag
    49141 gtgtgaccgt cgataccacc gcgccggtga tcagctttaa caccgtggcg ggcgatgatg
    49201 tgattaacaa cgtcgaacac actcaggcgc agattatcag cggcaccgcc acgggcgcgg
    49261 tcgcaggcga ccgcctggtg gtgaccatcg ccgggcagca gtatgtcacc agcaccgatg
    49321 ccagcggcaa ctggagcgtc ggcgtgcctg ccagcgtgat ttccggcctt gcggacggca
    49381 cggtgaccat cagcgccacc attaccgata gcgcaggcaa cagcagcacg cagacgcaca
    49441 acgtacaggt gaacacggcg gcagtgtcgc tttcggtcag caccatcagc ggcgataata
    49501 ttattaacgc cgccgaagcg ggcagtgcgc tgaccctgag cggcactggc actaatttcg
    49561 cgacaggtac agtggtgacc gtgttgctta acggcaaagg ctacagcgcc accattcaga
    49621 gcaacgggag ctggagcgtg aacgtgcctg cggcggatgt tgcggcgctc agtgatggca
    49681 ccagctacac ggttagcgcc tccgctcagg acagtgccgg aaacagcgcc acggcttcgc
    49741 gcagcgtggc ggtggatctc accgcgtcgg tgatcagcat taacaccgtt tcgacggatg
    49801 accgactcaa cgccgccgaa caacagcagc cgttaaccct taacggctcg accagcgcgg
    49861 aagtgggaca gacggtcacc gtcacctttg gcggcaaaac ctataccgcc acggttgctg
    49921 ccaatggcac ctgggcgctg aacgtgcctg cggcagatct ggccgcgctc gggcagggcg
    49981 cgcagaccat taccgccagc gtgaacgatc gcgccggtaa ccccggacag gccacgcacg
    50041 ccctgaccgt cgataccgtt gcgccaacgg tcaccatcgc cacggtggcg ggtgacgata
    50101 ttatcaacaa cgccgagcag cttgccgggc agaccatcag cggcaccacc accgccgaag
    50161 taggccagac ggtgaccgtc acttttaatg gtcagagctg gagcgcaacg gtcggcagcg
    50221 gcggaagctg gtcggtattt attccggcgc agcagtttgc cggattaagc gacggcagct
    50281 acaccatcag cgcgacggtt agcgatcagg ccggaaaccc cggcagcgcc agccgtggcg
    50341 tgacgctgaa cggcgacgtg cccaccgtca ccatcaacac ctttgcgggc gatgatgtgg
    50401 tgaatgcagc agaacacggc tcgtcgctgg tgatcagcgg caccaccacc gcgccggtcg
    50461 gacagacgct gaccctgacc ttaaacggca aaacctacac caccacggta cagaccggcg
    50521 gtagctggag ctacacccta ggcagcgccg atgttaccgc gctggcggac ggcaacgcct
    50581 acgtgattaa cgcctcggtg agcaatgcca ttggcaacac cggtagcagt aatcacacca
    50641 ttaccgtcga tctcagcgct ccggcgatgg gcattaatat cgattccctg caagccgaca
    50701 ctggccttag cgccagcgac tttatcacca gcgtcagtcc ggtagtggtc aacggctcgc
    50761 tcaccgccgc gcttgccagt aacgagacgg cgcaaataag tatcgacggc ggcacaacct
    50821 ggaccacgct taccgttacc ggcacgacct ggcgctataa cgacagccgc acgctgaccg
    50881 atggcaacta tctctatcag gtgcgggtga ttgacgcagc gggcaacgtt ggcgcgaccg
    50941 acagccagaa tgtggtgatc gacactaccg cgccagatcc cgcggtgaaa accatcgcca
    51001 tcagcgcgat caccaccgat acgggcttga tcactaacga ttttgtcacc agcgacacga
    51061 cgcttgcggt gagcggcacg ctgggggcga cgctttctgc cggtgagttc gcgcaaatca
    51121 gcattgatgg cggcaccacc tggcagaatc tttcggtaag cggtttgacc tggagctatc
    51181 ttgacgggcg tacgctgacc gatggcaact acaactacca ggtgcgggtg atcgacaccg
    51241 ccggaaatat cggcgcgacc gccagccaga tagtcactgt ggataccacc gcgccgctgg
    51301 cgagcaaaac catcgccatt gcgagcatca gcgatgacac cgggttaagc agcagcgatt
    51361 ttgttacacg cgacaccacg ctgaccgtgc gcggcacgct cggcgcggcg cttgccgccg
    51421 atgaacgggc gcaaatcagc ctcgacggcg gcgtcacctg gactacgtta accgttatcg
    51481 gcaccagctg gagctatgcc gatggtcgca cgcttactga cggcacctgg aactacacgg
    51541 tgcgggtggt ggatctggcg gggaacgttg ggcagaccgc aacgcaaaac gtggtggtcg
    51601 ataccaccag cccggaagcg gcgaaaagta tcaccattac cggcatcagc gatgacaccg
    51661 gaaccagcag cagcgatttt attaccagcg acaccacgct taccgtgcgc ggcgtgttgg
    51721 gcgcggcgct cggcgctaat gagttcgcac aaatcagtac cgacaacggc gcaacctggg
    51781 tgaacgtgac gctagcagca gacggcctga actggagtta cgttgacgga cgaaccctca
    51841 ccaacggcac caccacctgg caggtgcggg tggtcgatct ggcgggcaac gttggcgcaa
    51901 ccggcagcca gtcggcgcag attgacaccg ttaacccggc gcaggtgttg accatcgcca
    51961 gcatcagcac cgacacgggg agttcggcaa ctgactttat caccagcgat acctcgctca
    52021 cgctaaccgg ttcgctggga gcggggcttg ccagcggcga agtggcgcag attagccttg
    52081 atggcggcgc gacctggact acgctcacca ccaacggcac gcagtggacc tacactgaca
    52141 gccgcacgct gaccgacggc agctatgttt atcaggtgcg ggtgctggat ctggccggga
    52201 acaccggtcc ggtggtgtcg aaaacggtgg tggtcgatac gattaacccc accgccacgc
    52261 caggtattgt gtcgtatacc gatgatgtcg ggcagcggca ggggacgtta agcagttcgc
    52321 aggccaccga cgacactacg ccgctgctga acggtgtact ttccgcgccg cttgccagcg
    52381 gtgaagtggt ctatctctac cgtaacggcc tgctgttagg tgcggtaacg atggtcggcg
    52441 cactgaactg gacctacagc gacagcgggc tggtcagcgg tgcctatacc tacagcgcgc
    52501 gagtggtgga tttggcgggg aatatcacct cctccagtga ttttgtcctg acggtcgata
    52561 cctctattcc gaccacgctg gcgcaaatca ccagccagac cacgcgcgat actacgccga
    52621 ttatcagcgg ggtgatcacc gccgcgcttg ccagtgggca gtatgttgaa gtggtgatca
    52681 acggcaaaac ctacacctct gaaccgggcg gcgcggtagt ggtcgatccg gcgcacaaca
    52741 cctggtatgt acagttgccg gataccgatg cgctgacagt ttccgcgacc gcctataccg
    52801 ttactgcaca ggtaaaaagt tcggcgggta acggcaataa cgccaatatt agcaacggca
    52861 cggtgacggt taacgcggcg attgattaca caccgacctg gaccaccgcc agcaaaacca
    52921 ccgcctgggg gctgacctac ggcctcgact cgcacgggat gtggacggtg ctggcaaacc
    52981 agcaggtaat gcaatcgact gacccactca cctggtcgaa gaccgcgctg acgctgtatc
    53041 agagcggcaa caactacgcc accagctcca tcgccgatta cgaccgtaac ggcacgggcg
    53101 atctgtttat cacccgtgat gactacggta caggctatat caacggcttt accaataacg
    53161 gcgacggcac cttttccagc gctattcagg tcaccgtcgg caccctgacg tggtacggct
    53221 cgattgtggc atttgataaa gagggcgacg gctatctcga cttctggatt ggcgacgcgg
    53281 gcgggccgga ctccaacacc tttctgtgga acaacgcagg cacgctggta ggcaactcca
    53341 ccacgtcgaa cagcggcggt agcgccacgg tgggcggggc ggtgacgggg tatctttcgc
    53401 tcaacgaagg ttctggcgtc gatctgaaca atgacggcag gatcgacctg gttcagcaca
    53461 cctataacct gaacaactat tacacgctgt cttcgctcat caaccagggg aatgggacgt
    53521 ttgtctgggg gcagaacacc accaatacct tcctgagcgg ggcgggtagt ggcgctatga
    53581 gcagcagcgt ttccatgacc tgggccgatt tcgatggtga cggcgatatg gatctcttcc
    53641 tgcccgccag ccagggaaga gctaactacg gctcgctgtt attcaacacc aacggcgtac
    53701 tgggtagccc ggtggcggtg ggcgcaacgg caaccaccta cgccagccag tttagcctgg
    53761 cggtggactg gaaccatgac ggcctgatgg atatcgcccg tatcgcccag accggacaat
    53821 cgtatcttta caccaacgtt ggtggtgcca gcaactggac acaatcagcc ctcggcggca
    53881 gccagagcgg taccaccagc ggcgtggcgg caatggacta cgactgggac ggcgcggtgg
    53941 atgtgctggt gaccaaacag tcgggcagcg tgttcctgat ccgcaacacc aacacggtga
    54001 gctacggcac ttcgctacac ctgcgcatca ccgatcccaa cggcattaac gtctattacg
    54061 gcaataccgt gaagctgtac aactcggcgg gagtgctggt cgccacgcaa atcatcaacc
    54121 cgcagtcggg tatgggggtt aacgacacct cggcgctggt caacttctac gggctgaatg
    54181 ccggagaaac ctacaacgcg gtgctgatca aatccaccgg caccaccgcc agcaatatcg
    54241 accagacggt caacaccagc tggggcggtt tacaggccac cgatgccact cacgcttacg
    54301 acctcagcgc cgaagcgggt accgcgagca acaacggcaa gttcgtcggc accggttata
    54361 acgacacctt ctttgccacc gcaggcaccg atacctacga cggttccggc ggctgggtgt
    54421 acagctccgg caccggaacg tggctggcga acggcgggat ggacgtggtt gatttccggc
    54481 tttcgacggt gggcgtgacg gctaacttaa gcagcaccgc cgcgcaggcc accggtttta
    54541 acacctcgac atttaccaat atcgaaggca tttccggatc gaattttaac gacactctga
    54601 ccggcagcag cggcgataac caactggaag ggcgcggcgg caacgacacg ctcaacatcg
    54661 gcaacggcgg ccacgacacc ttgctctata aactgctcaa cgccagcgac gccactggcg
    54721 gcaacggttc ggatgtggtg aatggcttta cggtaggcac ctgggaaggc actgccgaca
    54781 ccgatcgcat tgatatccgc gagttgttgc agggcagcgg ctacaccgga aacggcaaag
    54841 ccagctatgt caacggcgtg gcgacgctgg atgcgcaggc cggaaacatc ggtgactttg
    54901 tcaaagtcac ccagagcggc agcgacacca tcgtgcagat cgaccgcgac ggcacgggcg
    54961 gcacttttgc gacaactaac gtggtcacgc tgacgggcgt gcacaccgac ctcgccactt
    55021 tgctggcgaa tcatcagttg atggtggtgt agataatgaa aaaaaatgcc cagtccgtgg
    55081 aagcctggct ggaggcgatg atcgccgtgg cgcgctatta tcggctcgat ttttctcagg
    55141 agaatgtgcg cgcgaccgtc aactgggagc gcgacagtaa gcgggaagag ttgctcaccg
    55201 acatggcgcg gcagttggga atggggttac ggctggtgga gttttccgcc gattcgctta
    55261 acccctggcg tctgccgctg atcgcggtgt ttgataatca gcagattggc gtgatcaccc
    55321 gtcgcgacaa tcatgacaat atcagcgttc agttcagcgg tgacgaaggg ctggaaacca
    55381 cgcttaacgt cgcggatgtc gaggataaaa tcgttgaact ggcgctactg cgcccgctca
    55441 gcgccatccc tgacgcgcgg gtagatgact atattcgccc gtatcaggcg aactggttct
    55501 ggagtctttc gctaaaagac tggcggcgct acggcgatat tatgctggcg tcactggtgg
    55561 cgaacgtgct ggcgctggcg gcgatgattt tctcgatgca ggtttacgac cgcgtcgttc
    55621 cggcgcagtc ctacccgacg ctatgggtgc tgtttgccgg ggtgatgatg gcgatcctgt
    55681 ttgagttctg tatgcgcatg gtgcgtacgc atctctcgga tgtgatcggc aagcgggcgg
    55741 atctgcgtat ttcggatcgc gtttttggtc acgcattacg gctgaaaaac aacgtgcgat
    55801 cgaaatccac cggctcgttt atctcgcaga tccgcgaact ggaatcggtg cgggagctga
    55861 ttacttccac cactatcggc gcggtggcgg atctgccgtt cttcctgctg tttgttttta
    55921 ttctgtggat gattggcggc tggctggtgc tggttgtttt gctggcgctg ccgttattgg
    55981 tgatccccgg cctgctggtg caacgcccgc tggcgcggct ggcgaacgaa ggaatgcgcg
    56041 agtcagcggt acgcaacgct acgctggtgg aagccgtgca gtcgattgaa gatattaaac
    56101 tgcttcgcgc tgaacagcgt tttcagaatc agtggaatca caccaacgac gtggcctcgt
    56161 ccatcagcat gaagcaacgt tttctcaccg ggctactgct cacctggacc caggaagtgc
    56221 aatccatcgt ctacgtcgtg gtgctgttgg tgggctgctt tatggtgatg aacggcgaca
    56281 tgaccactgg cgcactggtc gggacgacga tcctggcttc gcgcaccatc gcgccgctgt
    56341 cgcagatatc cggcgtgctc tcgcgctggc agcaggcgaa agtagcacgt aacggccttg
    56401 atgaactgat gaagcgcccc gtggatcagc cggaacatgg caaactggtg cataaagcgg
    56461 tgctgcacgg taattatcag ttcagcaacg cggtgtttta ttacgacgaa gaagagaaaa
    56521 ttgccgatgt ggcgatcggt aaactcaaca ttcaggccgg agagaaaatc gccattctcg
    56581 ggcgcaacgg cgcgggtaaa tcgacgctac tgcaaatgct cgccggaatg cgtatcgccc
    56641 agcaggggca ggtgttgctg gataacatca gtatcgggca gcttgatccg gcagatctgc
    56701 ggcgagacat ggggctactc agccagaccg ggcgactgtt ctttggttcg ctgcgggaaa
    56761 acctcaccat ggggatgccg gaagccagcg atgaagatat cgaacgcgcg ttaaccctca
    56821 gcggagcgct gccctttgtg cagaagcaga aaaacggcct caactacatg attcaggaag
    56881 ggggatttgg tctttctggc ggtcagcgac aaacgctgct gctggcgcgg ctactcatct
    56941 ctcaacccaa tatcgtcctg cttgatgaac ccagcgcctc gctggatgaa atggcggaag
    57001 cgtatttaat cgagcaactg aaacagtgga ttgggcatcg cactctgatc gtcgctaccc
    57061 accgcacggc gatgctgcaa ctggtggata ggataatcgt gatggatcag gggcggattg
    57121 tgatggacgg cgcgaaagag gccattttgc gtgaacaggg cgaacccacg gcgcgacggg
    57181 tggtgttgca ggaaaagaat aaggggtctg ccgcatgaac ggacaactgc aatataaccg
    57241 tggcctgact gaaccccgct tgccgcgttc ggcgctggcg gtacgcgtta ccgctgtgat
    57301 gctgctctgc tttcttggct gggcgtggta cttccagctt gatgaagtca ccaccggcag
    57361 cggcacggta gaaccctccg ggcgcgagca ggtggtgcag tcgctggaag gcggaatttt
    57421 gtatcatctc gacgtcaagg ttggcgatat tgttgaacag ggccagccgc tggcgcagct
    57481 taaccgcacc aaaacggaat ccgacgtcca ggaagcgatg tcgcggctgt acgctgcgct
    57541 ggcaacctcg gcgcggctgc gggcggaagt gagcaataaa ccgctggtct tccctgacga
    57601 actgaataaa ttccccgaac ttatcgagtc ggaaacggcg ttgtacaaca ctcgccgcga
    57661 cgggttgaac aaagccacca ccgggctgac gcaggggatc agtctggtca atcgcgaact
    57721 ggcgatgacc cagccgctgg taaaacaggg ggcagcgagc agcgtcgaag tactgcgcct
    57781 gcaacgccag gccaatgaac tggaaaacaa actcagcgac gtgcgcacgc aatactacgt
    57841 tcaggcgcgg gaagagttag cgaaagccaa tgcggaagtg gaaacccagc gttcggtgat
    57901 ccgtgggcgt gaagactccc tcaccaggct caactttact gcgccagttc gtggcattgt
    57961 tcaggatatt gacgtcacca cggttggcgg cgtcatcgcc cccggtggta aactgatgac
    58021 catcgtgccg ctggatgagc aactgctgat tgaggcaaaa atctccccgc gcgatgtggc
    58081 gtttatccat ccggggcaaa aatcgctggt gaaaataact gcctacgatt actccatcta
    58141 cggcggtctg ccgggagaag tagcggtgat atcaccagac accgtgcagg atgaagtgcg
    58201 gcgcgatgtt tattactatc gggtgtacat tcgcactttc agcaaccatc tggaaaacaa
    58261 aagcaaacag cagtttccga tattccccgg tatggtggcg acggtcgata tccgcaccgg
    58321 caaaaaaagc gtgctggatt atctgttgaa accctttaac aaagcacagg aggcgttgcg
    58381 cgaacgatga acattagcga tgtagcaaaa attaccggcc tgaccagcaa agccattcgc
    58441 ttctatgaag agaaggggct ggtgacgccg ccgatgcgca gcgaaaatgg ttatcgaacc
    58501 tacacgcagc agcatctcaa cgaattgacc ttactgcgcc aggcgcgcca ggtgggcttt
    58561 aacctggaag agagcggcga gctggtgaat ctgtttaacg acccgcagcg tcacagtgcc
    58621 gacgtcaaac gtcgcacgct ggagaaggtg gcggagattg aacggcatat cgaagaactg
    58681 caatctatgc gcaaccagct actggcgctg gcgaatgcct gccccggtga tgacagcgcc
    58741 gactgcccga ttatcgaaaa tctctccggc tgctgtcatc atcgggcggg gtgaaaatca
    58801 gttaattccc gtttgcaccg aaagaatatg ctgcaaactt tctaccataa tgcgccccga
    58861 ctgaaataag ccttcctgga tttcgttgtt aacattcaca gcgtgaacca gggaggctaa
    58921 taatactact tgcaagaagg caaaaataaa cgcaccgata atcataatat gcgtcagggg
    58981 tattttcctt tgctccttca ttttaaataa cccacaaact ggccttcttt cattaaggcg
    59041 acttttcctg tgtgatgata aacaatgacg ttatacagac ctgactgata aacagtggca
    59101 tctttattgt cttcatcaat tgcacataat gaatcattga gataagcaaa gtttttactg
    59161 ctcattctaa aggtcatgcc aaatccttta taaatggctt tctttgccgc ctgggcatgt
    59221 tgtgcggctt ctgcattttt aaatgcctgc gcttcggaag cacttaaaat agccgtctgg
    59281 cgctgctgtt ctgcgagata acaggtgtca tggctgacgc cctgcgcctg gcaatctgcg
    59341 atgcgggaag cgggtgagct acagccagcc agtaatataa ccgccagcag ggaaaacgat
    59401 ttaatggagc tattcatcat tgttaacctt aaataaactg cggaatattg ctatacccgt
    59461 catacttcaa gttgctgcaa ctcgaattat ttagagtata ataagaaatt gctattaaaa
    59521 ttagaacgaa taccccacgc cgacacccgc gccgaaatca gattgtgtat ccgcagagac
    59581 tcccgcttta attaccgtat gaattccagc attaaacgaa gcaccgacag ataccgcaga
    59641 ttcattgttg aatgtacccg ccccggcaga caccatcata aaatcacctg tcttaacttg
    59701 aggctgtgaa gcaatagcga cggctgacgc aatacccgca ttagcctctt tacggttatc
    59761 ttccacttcg tgtttcaacg aactgaagtt gttattgagc gaattgattt gctggctgtt
    59821 atctgcaaca cgtttttgcg tgtttgttaa ctgctggtgc gtttgggcag aatcttgtgt
    59881 cagactttcg atttgctcat gtgcctgggc cgagtcttgc gcaaggtttc gaatttgttc
    59941 accgtattgc acgctatcga tttgctgttg cgtcgcgaca ttggaagcca gttcattaat
    60001 tctctgctcc tgatcagcgg ttgaacgttc attggcttca ttttgcgccg tcagtttggc
    60061 attaatcatc ttttgataat gcgcagttga ctggggatcg ccgattgcag cgaccgtttt
    60121 ttctgtggct gcgatctgct tctgttgctc tgtaatctgc gtttgttggg cttcagactg
    60181 tgattgttgc gcatcagcag tggctgtctg aagagcgtta atcatgctct gataacgagc
    60241 agcggtcttt acatcacccg tggcggcaac ggttttttgc gtggcagtga tttgttcctg
    60301 ctgttcggta atcgccgtac tgtttttttc aatctggttt ttgttgtcgc tgacgacacc
    60361 ggaaagtttc tggccattca tcagcgcggt attagcattc gcgttggcat gatcggcact
    60421 tgctttaaca tctgcgacat catctttcat tgcactcaca tcggtgcgga cagcggcaac
    60481 atctttttgt gtgtcggaaa tggccgactt attggcgtcg atatccgttt gcgccaccat
    60541 aatggcatgg tgcgcgtcat cggctttaag cgaagcccag ttagcagagt gctgggcatc
    60601 ctgaattgac gagtcctgtg cggcatcgcg atgtatttgg ttctgctcac ttaacgccat
    60661 ctgttcctgc tgttcagcaa tagaggagct gtttttttca atctggtttt tgttgtcgct
    60721 cacggcaccg gagagtttca ggccattcat cagggcggta ttggcgtttg tgttggcatg
    60781 agcggcgctt gctttaacat ctgcgacatc tttctgagta tcagcaatag ctcccttgtt
    60841 cgcatcaatt gcagattgcg cagtggtaag cgcttttttg gcgtcatctg catttgccgc
    60901 cgcaatgctt gcgatattgt gtgcgttctg aattgcggta tcttgcgccg cgtcgcgatc
    60961 agcctgtttt tggtcggtga tgttgctgtc agtctgtacg gctgtactgg cagggtaaat
    61021 attatcgcct gtctgtgggt ccatatatcc atgagaaaac aggtaatctt gcgcggcttc
    61081 tgtattacct gtccgaatca tgaagcgatt gattttttga tcgatttcat catgagacat
    61141 acctgggtga atcgctgtca gttctgtcgc tgccgcttca accgtatcat ctgcaaaagc
    61201 agatgggctc acaatggtag caacaacgac tgccagtaaa gacaacttta cagatttca
//
LOCUS       DAZGCQ010000015        57523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000015 DAZGCQ010000000
VERSION     DAZGCQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.015"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..578
                     /locus_tag="ACHK07_001029"
     CDS             <1..578
                     /locus_tag="ACHK07_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO8101400.1"
                     /translation="AEMKELAKIFSFFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSF
                     HEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            575..1066
                     /locus_tag="ACHK07_001030"
     CDS             575..1066
                     /locus_tag="ACHK07_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO8101401.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGSSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNLHDFKK
                     DNF"
     gene            complement(1108..2058)
                     /gene="corA"
                     /locus_tag="ACHK07_001031"
     CDS             complement(1108..2058)
                     /gene="corA"
                     /locus_tag="ACHK07_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO8101402.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2210..2266)
                     /gene="ysgD"
                     /locus_tag="ACHK07_001032"
     CDS             complement(2210..2266)
                     /gene="ysgD"
                     /locus_tag="ACHK07_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO8101403.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2428..3192
                     /gene="yigE"
                     /locus_tag="ACHK07_001033"
     CDS             2428..3192
                     /gene="yigE"
                     /locus_tag="ACHK07_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO8101404.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYVKGGAIPWQRYPFVTMISAERKG"
     gene            3275..4177
                     /locus_tag="ACHK07_001034"
     CDS             3275..4177
                     /locus_tag="ACHK07_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO8101405.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTGRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAISFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(4222..6384)
                     /gene="uvrD"
                     /locus_tag="ACHK07_001035"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(4222..6384)
                     /gene="uvrD"
                     /locus_tag="ACHK07_001035"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO8101406.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(6468..7184)
                     /gene="yigB"
                     /locus_tag="ACHK07_001036"
     CDS             complement(6468..7184)
                     /gene="yigB"
                     /locus_tag="ACHK07_001036"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO8101407.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(7184..8080)
                     /gene="xerC"
                     /locus_tag="ACHK07_001037"
     CDS             complement(7184..8080)
                     /gene="xerC"
                     /locus_tag="ACHK07_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO8101408.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(8077..8784)
                     /gene="yigA"
                     /locus_tag="ACHK07_001038"
     CDS             complement(8077..8784)
                     /gene="yigA"
                     /locus_tag="ACHK07_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO8101409.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8781..9605)
                     /gene="dapF"
                     /locus_tag="ACHK07_001039"
     CDS             complement(8781..9605)
                     /gene="dapF"
                     /locus_tag="ACHK07_001039"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO8101410.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLAYRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9642..9845)
                     /gene="yifL"
                     /locus_tag="ACHK07_001040"
     CDS             complement(9642..9845)
                     /gene="yifL"
                     /locus_tag="ACHK07_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8101411.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10004..10146
                     /locus_tag="ACHK07_001041"
                     /pseudo
     CDS             10004..10146
                     /locus_tag="ACHK07_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10308..10628
                     /gene="cyaY"
                     /locus_tag="ACHK07_001042"
     CDS             10308..10628
                     /gene="cyaY"
                     /locus_tag="ACHK07_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO8101412.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VRFR"
     gene            complement(10668..13214)
                     /gene="cyaA"
                     /locus_tag="ACHK07_001043"
     CDS             complement(10668..13214)
                     /gene="cyaA"
                     /locus_tag="ACHK07_001043"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO8101413.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPDRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            13601..14542
                     /gene="hemC"
                     /locus_tag="ACHK07_001044"
     CDS             13601..14542
                     /gene="hemC"
                     /locus_tag="ACHK07_001044"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO8101414.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14539..15279
                     /gene="hemD"
                     /locus_tag="ACHK07_001045"
     CDS             14539..15279
                     /gene="hemD"
                     /locus_tag="ACHK07_001045"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO8101415.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            15301..16506
                     /gene="hemX"
                     /locus_tag="ACHK07_001046"
     CDS             15301..16506
                     /gene="hemX"
                     /locus_tag="ACHK07_001046"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO8101416.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMNNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQAAPQAD
                     TPAAAPQGE"
     gene            16509..17705
                     /gene="hemY"
                     /locus_tag="ACHK07_001047"
     CDS             16509..17705
                     /gene="hemY"
                     /locus_tag="ACHK07_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO8101417.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            18384..20039
                     /gene="aslA"
                     /locus_tag="ACHK07_001048"
     CDS             18384..20039
                     /gene="aslA"
                     /locus_tag="ACHK07_001048"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO8101418.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     DYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(20207..21442)
                     /gene="aslB"
                     /locus_tag="ACHK07_001049"
     CDS             complement(20207..21442)
                     /gene="aslB"
                     /locus_tag="ACHK07_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8101419.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(21589..21665)
                     /locus_tag="ACHK07_001050"
     tRNA            complement(21589..21665)
                     /locus_tag="ACHK07_001050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21629..21631),aa:Pro,seq:tgg)
     gene            complement(21708..21794)
                     /locus_tag="ACHK07_001051"
     tRNA            complement(21708..21794)
                     /locus_tag="ACHK07_001051"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21758..21760),aa:Leu,seq:cag)
     gene            complement(21815..21890)
                     /locus_tag="ACHK07_001052"
     tRNA            complement(21815..21890)
                     /locus_tag="ACHK07_001052"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21855..21857),aa:His,seq:gtg)
     gene            complement(21949..22025)
                     /locus_tag="ACHK07_001053"
     tRNA            complement(21949..22025)
                     /locus_tag="ACHK07_001053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21989..21991),aa:Arg,seq:ccg)
     gene            complement(22128..23513)
                     /gene="yifK"
                     /locus_tag="ACHK07_001054"
     CDS             complement(22128..23513)
                     /gene="yifK"
                     /locus_tag="ACHK07_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8101420.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(23704..24444)
                     /gene="wecG"
                     /locus_tag="ACHK07_001055"
     CDS             complement(23704..24444)
                     /gene="wecG"
                     /locus_tag="ACHK07_001055"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO8101421.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(24447..25799)
                     /gene="wzyE"
                     /locus_tag="ACHK07_001056"
     CDS             complement(24447..25799)
                     /gene="wzyE"
                     /locus_tag="ACHK07_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO8101422.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILIGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(25796..26875)
                     /gene="rffT"
                     /locus_tag="ACHK07_001057"
                     /gene_synonym="wecF"
     CDS             complement(25796..26875)
                     /gene="rffT"
                     /locus_tag="ACHK07_001057"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO8101423.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDNCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(26872..28122)
                     /gene="wzxE"
                     /locus_tag="ACHK07_001058"
     CDS             complement(26872..28122)
                     /gene="wzxE"
                     /locus_tag="ACHK07_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO8101424.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVIGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(28124..29254)
                     /gene="rffA"
                     /locus_tag="ACHK07_001059"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28124..29254)
                     /gene="rffA"
                     /locus_tag="ACHK07_001059"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO8101425.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(29259..29933)
                     /gene="rffC"
                     /locus_tag="ACHK07_001060"
                     /gene_synonym="wecD"
     CDS             complement(29259..29933)
                     /gene="rffC"
                     /locus_tag="ACHK07_001060"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO8101426.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(29911..30792)
                     /gene="rfbA"
                     /locus_tag="ACHK07_001061"
     CDS             complement(29911..30792)
                     /gene="rfbA"
                     /locus_tag="ACHK07_001061"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8101427.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(30811..31878)
                     /gene="rffG"
                     /locus_tag="ACHK07_001062"
     CDS             complement(30811..31878)
                     /gene="rffG"
                     /locus_tag="ACHK07_001062"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8101428.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(31878..33140)
                     /gene="wecC"
                     /locus_tag="ACHK07_001063"
     CDS             complement(31878..33140)
                     /gene="wecC"
                     /locus_tag="ACHK07_001063"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO8101429.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(33137..34267)
                     /gene="wecB"
                     /locus_tag="ACHK07_001064"
     CDS             complement(33137..34267)
                     /gene="wecB"
                     /locus_tag="ACHK07_001064"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO8101430.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(34323..35369)
                     /gene="wzzE"
                     /locus_tag="ACHK07_001065"
     CDS             complement(34323..35369)
                     /gene="wzzE"
                     /locus_tag="ACHK07_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO8101431.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(35381..36484)
                     /gene="wecA"
                     /locus_tag="ACHK07_001066"
     CDS             complement(35381..36484)
                     /gene="wecA"
                     /locus_tag="ACHK07_001066"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO8101432.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(36724..37983)
                     /gene="rho"
                     /locus_tag="ACHK07_001067"
     CDS             complement(36724..37983)
                     /gene="rho"
                     /locus_tag="ACHK07_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO8101433.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(38068..38169)
                     /gene="rhoL"
                     /locus_tag="ACHK07_001068"
     CDS             complement(38068..38169)
                     /gene="rhoL"
                     /locus_tag="ACHK07_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO8101434.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(38310..38639)
                     /gene="trxA"
                     /locus_tag="ACHK07_001069"
     CDS             complement(38310..38639)
                     /gene="trxA"
                     /locus_tag="ACHK07_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO8101435.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            38770..40035
                     /gene="rhlB"
                     /locus_tag="ACHK07_001070"
     CDS             38770..40035
                     /gene="rhlB"
                     /locus_tag="ACHK07_001070"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO8101436.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            40169..41653
                     /gene="gppA"
                     /locus_tag="ACHK07_001071"
     CDS             40169..41653
                     /gene="gppA"
                     /locus_tag="ACHK07_001071"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO8101437.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(41700..43721)
                     /gene="rep"
                     /locus_tag="ACHK07_001072"
     CDS             complement(41700..43721)
                     /gene="rep"
                     /locus_tag="ACHK07_001072"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO8101438.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            43938..44387
                     /locus_tag="ACHK07_001073"
     CDS             43938..44387
                     /locus_tag="ACHK07_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO8101439.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFITTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            44582..44863
                     /gene="ppiC"
                     /locus_tag="ACHK07_001074"
     CDS             44582..44863
                     /gene="ppiC"
                     /locus_tag="ACHK07_001074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO8101440.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(44909..46384)
                     /gene="ilvC"
                     /locus_tag="ACHK07_001075"
     CDS             complement(44909..46384)
                     /gene="ilvC"
                     /locus_tag="ACHK07_001075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO8101441.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            46534..47427
                     /gene="ilvY"
                     /locus_tag="ACHK07_001076"
     CDS             46534..47427
                     /gene="ilvY"
                     /locus_tag="ACHK07_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO8101442.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(47479..49023)
                     /gene="ilvA"
                     /locus_tag="ACHK07_001077"
     CDS             complement(47479..49023)
                     /gene="ilvA"
                     /locus_tag="ACHK07_001077"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO8101443.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(49026..50876)
                     /gene="ilvD"
                     /locus_tag="ACHK07_001078"
     CDS             complement(49026..50876)
                     /gene="ilvD"
                     /locus_tag="ACHK07_001078"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO8101444.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(50941..51870)
                     /gene="ilvE"
                     /locus_tag="ACHK07_001079"
     CDS             complement(50941..51870)
                     /gene="ilvE"
                     /locus_tag="ACHK07_001079"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO8101445.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(51890..52153)
                     /gene="ilvM"
                     /locus_tag="ACHK07_001080"
     CDS             complement(51890..52153)
                     /gene="ilvM"
                     /locus_tag="ACHK07_001080"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO8101446.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(52150..53796)
                     /gene="ilvG"
                     /locus_tag="ACHK07_001081"
     CDS             complement(52150..53796)
                     /gene="ilvG"
                     /locus_tag="ACHK07_001081"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO8101447.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLSTTKMPATCTLKGLGAVAADYSYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLSDEHAWRYDHPGDTIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(53799..53849)
                     /gene="ilvX"
                     /locus_tag="ACHK07_001082"
     CDS             complement(53799..53849)
                     /gene="ilvX"
                     /locus_tag="ACHK07_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO8101448.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(53936..54034)
                     /gene="ilvL"
                     /locus_tag="ACHK07_001083"
     CDS             complement(53936..54034)
                     /gene="ilvL"
                     /locus_tag="ACHK07_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO8101449.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            54387..55907
                     /gene="yifB"
                     /locus_tag="ACHK07_001084"
     CDS             54387..55907
                     /gene="yifB"
                     /locus_tag="ACHK07_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO8101450.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIANHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSNIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(55932..56270)
                     /gene="maoP"
                     /locus_tag="ACHK07_001085"
     CDS             complement(55932..56270)
                     /gene="maoP"
                     /locus_tag="ACHK07_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO8101451.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            56389..57228
                     /gene="hdfR"
                     /locus_tag="ACHK07_001086"
     CDS             56389..57228
                     /gene="hdfR"
                     /locus_tag="ACHK07_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO8101452.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(57324..57399)
                     /locus_tag="ACHK07_001087"
     tRNA            complement(57324..57399)
                     /locus_tag="ACHK07_001087"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57364..57366),aa:Trp,seq:cca)
     gene            complement(57408..57484)
                     /locus_tag="ACHK07_001088"
     tRNA            complement(57408..57484)
                     /locus_tag="ACHK07_001088"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57448..57450),aa:Asp,seq:gtc)
BASE COUNT        14227 a        15473 c        14516 g        13307 t
ORIGIN      
        1 ttgcagaaat gaaagaacta gcaaaaattt tttccttttt tggacaatat aaaacactta
       61 tcgaagatct gattgaacat tttagatatg gaaatggcag caattttcat agccaacaat
      121 taaatttatc ttttcatgaa aaaataaata aatatggtta taatagtcca atacgaataa
      181 tcaaagaatg tatcgaaaat ggcattaatt caactccctc gacaggatac caacctctta
      241 tcctgcaaag cattaaaaca aaattacttt catcaagact aaataaattt aatgacttcg
      301 aggacagttt taatggctta ggaatcagtg ttcatgatat ttcagctcaa aaaatatctc
      361 ttttaagttt tcaaaaatac gctataggat ggagtgcgac aattcatttt gtagcacagg
      421 accacttcgg tcttgatgta actgatatca aaaacaagac ttacagcaaa tatagatttt
      481 ttcgcatatg gttcttttta caacggcata aagactttgc tttcaaacct tttttcacca
      541 attttaacac tatcgaaaga atcgaaaatt atctatgaaa agcaaactcc agaaaattat
      601 attatgttta tttcttctat gctgcatcta taatttatgg acattgcgtc ctgtacagat
      661 tttgtataca tactctgatg ctggcagttc agtctttctg gtcgttgatc atctgccatg
      721 gactgacagc gataaaataa attggtatct gaagcatcag aatgaaatta aaaatcaaca
      781 tcccctacca gaaggtagtt ggcatacctg gtatgtcatt gatatcggta atggcttcac
      841 tgactataaa aaatatatcg aagggccgta tgaagatctc tactgtttcc cgacaattaa
      901 gagcaatgat aattgcatcg tgaaaaatta cttaatggtt attaatgaat acccttatcg
      961 taacactcat attggtatta acgattttac tgagtatcaa ttaacacaag aaaataaaat
     1021 agagcgagtt ttcaatctgc atgactttaa aaaagacaat ttttagaaat aagccggata
     1081 aaaaaatagc tttatccggc actcatatta caaccagttc ttccgcttaa agtacagata
     1141 cggtgccagg cccgcgagga tcataaagat aatcgcgcct gggtagccga aactccactt
     1201 cagttctggc ataaactcaa agttcatccc gtagctggaa gcaaccagcg ttggcggcag
     1261 gaacacgacg gataccaccg agaaaatttt gatgatgcgg ttctgctcga tgttgataaa
     1321 acccattgcc gcctgcatca ggaagttcac cttctggaac agggattcgt tatgcggcag
     1381 cagggattcg atatctcgca ggatttcacg cgcctgctcc agttgaccac ccggtaaacg
     1441 cgccttacgc accaggaagt tgagcgcacg ctgggtatcc atcagacaca ggcgcacttt
     1501 ccagccgata tcttccagtt ccgccagagt ggagagcgcc tcgtcgtact catcgccctg
     1561 atgcccttcc ataatcaccc ggctcaactg ctccaggtcg ctatagatat tttcaatttc
     1621 atctgccaac tgttcgattt tggtttcgaa cagatccagc agcagctcgt aggcgttacc
     1681 gtccaccatt gactggctac gggcgcgcat acgatacaga cggaatgcag gcagttcacg
     1741 ctcacgcaga gtaaacagac gaccatcacg gatggtaaat gccacagtgg agttaccggc
     1801 gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata tgcaggccgt catcgtcttc
     1861 aaagaaacgt gccgatgctt cgatgtcttc cagttccggg cgggttgcca ggctctggcc
     1921 aagttcagat tgtacgcgca gtcgctcgtc gtcgtccggt tcgacaagat caatccatac
     1981 tgcatttaca aggggttgtg actcttcgac ttccagccgg gtcagtcggt tattttccag
     2041 ttgaaatgcg ctcagcatga ccgggactcc caatgcttaa aatatcggac agttcggtgg
     2101 gcacacagaa acaaattggg tttcagacca ttaaacagcc tgactcagcg cgacgggaaa
     2161 aattgaggtc gctgacaacc gctaaggcta tcagcaaaaa gggatagcct taggagttga
     2221 tcctggatga caggatagtg agccagtatc tactgggtgt gtccaaggcg aatgtcctct
     2281 tagagtgatc gtgcgcgcat gttacgccag caaaattttg ccgtcaacac gcaacagaac
     2341 accacagaac aatagttgaa cttcgcgctt tttggtactc gtcaaggtcg ctaacaggat
     2401 aaaaaaatcc cacatccagg acctataatg gcgcatcggc tactcattgg taaaggaatg
     2461 atcaccttga atctcaaacg gatttttctc gccctcacct tacttccctt gtttgctgtt
     2521 gccgctgatg attgcgcact ctcagatccg acgctgaccg tacaggcgta taccgttaat
     2581 cctcaaacag agcgggtgaa aatgtactgg caaaaagcca atggcgaagc gtggggaact
     2641 ttacatgctc tgctggcgga tatgaatagt cagggtcagg tgcagatggc gatgaacggc
     2701 ggcatctatg atgaaagcta tgcgccgctc ggtttgtaca tcgaaaacgg tcagcagaag
     2761 gtggcgttaa atctcgcttc aggtgaaggg aatttcttta tccgtcctgg cggcgtgttt
     2821 tatgtcgcgg gagataaagt cggcatcatt cgtctggatg ccttcaaaac cagtaaagag
     2881 attcagtttg cggtgcagtc agggccaatg ttgatggaaa acagtgtaat taatccgcgt
     2941 attcatccca acgtcgcctc acgtaaaatt cgtaacggtg ttgggattaa taaacatggg
     3001 aatgccgtgt ttttgttgag ccagcaggcg acaaattttt atgattttgc ctgttatgcc
     3061 aaagcgaaac tgaacgttga gcagctgctt tatcttgatg gcacgatttc acatatgtat
     3121 gtgaaaggcg gggcaatccc gtggcaacgc tatccttttg tcaccatgat ttccgccgag
     3181 cggaaaggtt aagcattgta cactcgagga ggccagacaa actggggctg gctacacagc
     3241 tggaaaatca tcagataaat gaagggaaac aaaaatgaaa aaaacgctcg caacgttatt
     3301 tttattaacg tgtctgggat catcatccgc ttatgcggat aacgctttga ttttacaaac
     3361 tgattttagc ctgaaagatg gtgccgtctc ggcgatgaaa ggggtcgcat tcggtgttga
     3421 ccataatctt aaaatctttg atttaacaca cgaaattcca ccgtataaca tctgggaagg
     3481 cgcttaccgc ctgtatcaga ccgccagtta ttggccacaa ggttcggtgt ttgttagcgt
     3541 ggtcgatcca ggtgtaggta caggccgtaa atcggtagtg ctgaaaacca aaaacggtca
     3601 atacttcgtt tcaccggata acggtacgct cactttggtg gcggagtcgc tgggtattga
     3661 gagcgtccgg caaatcgatg aaaaaaccaa ccgcctgaaa ggttccgaaa agtcatatac
     3721 cttccatggt cgcgatgtat atgcctacac aggggcgcgt ctggcttccg gcgcgatttc
     3781 ctttgagcag gtggggccag agcttccggc aaaagttgtc gaactgcctt atcagaaagc
     3841 gaaagcgaca aaaggtgaag tgaagggcaa tatcccgatt ctcgatattc agtacggtaa
     3901 cgtctggagc aatatcagcg acgagttgct gaatcaggca ggaatcaaac tcaacgatac
     3961 gttatgtgtg accatttccg aaggttccga gaaaaaatac gaagggaaaa tgccgtatgt
     4021 cgccagcttc ggcgatgtgc cggaaggaca gccgatggtg tatctgaaca gcttattgaa
     4081 tgtttccgtg gcgctgaata tggataattt cgcgcagaaa catcaggttg cgtctggtgc
     4141 tgactggaat attgatgtga agaaatgtga taagtaaggt gctgatgccg gatgcggtgt
     4201 ggcgcaccgc atccggcaac gttataccgt ctccagccgg gcgtaagccg ccaccagcca
     4261 tttaataccc tggccctgga atgccacctg caaacggcta tgctcgccgc tgccttccat
     4321 attgacgata gtgccttcac caaacttggc gtggcgcacg cgctggccaa gtttgtagcc
     4381 gctgtcgttc tcgaccatcg gcgtacccat acgctgatgg ctgaccgggc ggcttaccgt
     4441 ggcgcgcagg cgcacctctt ccacacactc ttccggcaac tcaccgataa agcgcgacgg
     4501 gcgatggtaa acctctttgc catacagacg gcgagtttcc gcgtaggtca gcgtcagttt
     4561 ctgcatcgca cgggttacgc caacgtaggc cagacggcgt tcttcttcca gacgaccgcc
     4621 ttcatccagc gacatctggc ttgggaacat gccctcttcc ataccgacga taaacacctg
     4681 cgggaactcc aggcctttcg ccgagtgtag cgtcatcaac tgcaccgcgt cctgccaggt
     4741 atccgcctgc ccctcgcccg cttccagcgc cgcatgggag aggaacgcct gcagcggcat
     4801 taaatcttcg tcttcttcgt tgtagctgaa ctggcgcgtt gccgtcacca gttcctctaa
     4861 gttttcgata cgcgtctgac ctttttcgcc cttctcctgc tcgtacatgg tacgcaggcc
     4921 ggagtcttta attacccggt cagtctgtac atgcagcggc atatcggcag tttcctgcgc
     4981 taaggcgtcg atcagttcca taaaccgctg caaggcactg gctgcacgtc cggcgagggc
     5041 tttttcctgc aacagttcac gacatgcctg ccagagtgtt aactggcgat cgcgcgatgt
     5101 ctgacgtacc acgtccagcg tccggtcacc aataccccgc gttggtgtat tcactacgcg
     5161 ctcaaaggcc gcgtcgtcgt tgcggttggc aatcaggcgc agatacgaga gcgcatcttt
     5221 gatttcctgg cgttcgaaga agcgcatccc gccgtaaata cggtacggca tactcgcctg
     5281 taataaggcc tcttccagta cacgcgactg ggcgttgctg cggtagagaa tggcgcactc
     5341 ggcaagcgct ccgccgttgt cctgccaggt tttgatgcgg ttaaccacaa aacgcgcttc
     5401 atcgagttcg ttaaaagcgc aatagaggga aataggctca ccgtccgcgc catcggtcca
     5461 cagtttttta cccagacgcc cgttattgtt ttcaatcagg gcgttagcgg cgctcagaat
     5521 attgctggta gagcggtagt tttgctccag acgaatagtt tcggcaccgg ggaaatcatt
     5581 aaggaaacgc tgaatattct ccacctgcgc cccgcgccag ccgtagattg actggtcgtc
     5641 atcaccgacg atcatcactt tgccggtgtc gcccgccagc aggcggatcc acgcgtactg
     5701 aatgttgttg gtatcctgga attcgtccac caggatgtta gtaaaacgtt cgcggtagtg
     5761 ttgcaggata tgcggcttgt taagccacaa ctcgtgagca cgcagcagca gctcggcgaa
     5821 gtccaccagg cccgcacggt cgcacgcttc ctgatacgcc tgatacactt tctgccaagt
     5881 ctgctccacc ggattaccgt agctttgaat atgatgcgga cgtaagcctt catctttctg
     5941 gctgttgatg taccacattg cctggcgcgg cggccactgc ttctcgtcga ggttcatggc
     6001 tttgatcaga cgcttaagca ggcgtagctg gtcttcactg tcgaggatct ggaaatcctg
     6061 cggcagattg gcgtccatat ggtgcgcacg cagcaggcgg tgcgccagcc cgtggaaggt
     6121 gccgacccac atgccgccct ggctggtgcc catcagttgc ccgatacgat gacgcatctc
     6181 cgccgccgct ttgttggtaa acgtcacagc cataatcgag tatggcgagc agttttccac
     6241 gctcatcagc caggcgatac gatgcaccag tacgcgcgtc ttaccactgc ccgcgcccgc
     6301 cagcaccaga aggttgctgc gtggcgcggc caccgcttcg cgctgtttgt cattaaggct
     6361 gtcgagcagg taagaaacgt ccattggcac cgccgcgtaa aaataggttg ggcggagaag
     6421 cgcaacgccc tccgccaaaa agctgggtat atatacagat ttgctgatta tatcagcgag
     6481 gtcagagatg ccaaccggga aatttccaga tgcggcagta aacggctatc ccaggtttgc
     6541 atcagatcgc cattttccgg tctgatccaa caagcctgca ttccgctgcg aattgcccca
     6601 cccacgtcag tggtgagatc gtccccaaca tgtaagatct cgccgatcgg cacgttgagt
     6661 ttttccgcag ccaaaaagta catatcgctg aacggttttg agcgcccgtg cgggccagcg
     6721 cgcagcacaa actcaaaata atcccccaaa ccaaacagtt ccggctgggc gttaccgttg
     6781 gtgatcgcca ccagcggcca ttttttcgcc agctgtttta aggtgtcgtg agtttgctgc
     6841 gggacgtcga ttcggctgcg ccatttggca aagttaatca ttgctgcgtg tgcgcctgca
     6901 ctggcttctt cggcactcag cccggcgtcg agcatcgctt gttcaatcga acgaaaacgc
     6961 caacgcgtca cgtcgtgata aatctcgggt tccgcttccc gtaccgcctg gcgcaggcgt
     7021 tgcagatctt cattctggaa gctgcgcagc gccggatgat aattttgcac aaaggtaagc
     7081 gcctctcgct cggtgcgcaa aatcaccgga cggttatcgt aaagggtatc atccaggtca
     7141 aaggtgagcg ccgagatgcg ccccaaaggc cggtaaaaac gcattatttc ccccgtttgg
     7201 cgcgtggatg cgccgcatcg tacaccgagg caaggtgttg aaaatcaaga tgagtataga
     7261 tttgcgtggt ggagaggttg gcatgaccca gcagctcctg cacaccacga agatcgccgc
     7321 tcgactccag catatgcgta gcgaacgagt gacgtaattt atgcgggtga acgtgattat
     7381 tcagcccttg ttttatgccc cattcggcaa agcgtttctg cacattacgc gcggagatac
     7441 gcttgcccag tttcgacaga aaaagcgcgt cgtcttcgct accaaacagg tcgcgcaaat
     7501 caagccagtg ctcaatccac gccacagcgt tgcgaccaat cggcaggcgg cgctctttgc
     7561 tgcctttccc cattacccac acttcaccag actccaggtc gaggtgtttg atatccagcc
     7621 ccaccaactc ggaaagacgc agaccagcgc cgtacatcac ttccagcatt gcacggtcgc
     7681 gtacagcgag gggatcgttg atatcaatat ccagcagccg attcatatcg tcgacgtcga
     7741 tgtttttcgg cagatgacgc ggcgctttcg gtgccgaaac acctttcgcc gggttagctt
     7801 tgagttcgtt ctggctgacc agccagtcaa aaaagctacg tagcgcagaa agccgtaacg
     7861 cgaggcttgc tgctcccagc cctttacggc gactgcgtac agcaaaattg cgcaccatcg
     7921 ctgcatcgca ttgttgccag ctttgcaggc cgttttcgct ggcaaaattg atgatcgcct
     7981 caagctgacg ctggtagtta agtagggtta tcgggctaag ctgacgctcc acgctcagat
     8041 aacgtaggta gcgttctaca tcggtgtgta aatcggtcat acgcgttcaa tccaacgctc
     8101 cagaagctcc ggcaacatca gcgcaatttc atgaagtaac tgcgttccct gcccttgttg
     8161 atagtgactg gcatcgcgac tggtaaacag cacgacaccc aaatcagcat cgcttcccag
     8221 catcgacatc gccaccgatc ccaccgcttt cgcttccggt agcaccacca gcagctctgg
     8281 tccgttaagc ggcccaagat agtgctgttc ctgccccaaa cgctgaatac gcagcggttc
     8341 gaaagactga cggcttaatg ccagatgagt gtggttcgac ggcgcaccta agcgccagcg
     8401 atccggaaac aggcgcagac tcgcacctgc caggccgaga tcgcgcgccc agcggtgaaa
     8461 gcgcatcagc atatcgtcga gactgctggc ggcggtgaga ctgcgctgca ggtagagtaa
     8521 gcgataaaac aggccttcgt tggcgatagc ctgttccatc aacagcgcca tgttctcttc
     8581 cagaacatga atatgattac gtgcgcgggc catgtgccac tcgaccaacg aaacggtgcc
     8641 gcgtaccgga tgcggcacac gtatcgcttc tactgcgcgc gcattgcgga taaaaaactc
     8701 aggattttta atcagataat cgacaaccgc ccggtcatca agctccgtga gtgtttcctg
     8761 cagttcttcc cctggttgct tcatagatga ataaatccgt cgtagacatg taccgccggg
     8821 ccagtcatat ataacgggtg acccggacct ttccaggcga tatcaagacg accgccgggg
     8881 agttccacgc gtacttcttc ggccagcaaa ccttgctgaa tccctaccgc caccgccgca
     8941 catgcgccgc tgccgcaggc ctgggtttct cctgccccac gctcataaac gcgtaaacga
     9001 atatgctcgc gcttaaccac ttgcataaaa ccgatattgg ctcgctccgg aaaacgctcg
     9061 tggctttcca gaacaggacc aagcgtttct accgccgcgg tatcgacatc atcgacctga
     9121 atcacgcaat gcggatttcc catcgacacc acgccgcata agattgtctg ctcggcggcg
     9181 cgcataatgt aggtcttttc cgctttgtta gcgcgaaacg gcacagcgga aggttcgaag
     9241 ttgggttcgc ccatatttac gcggaccaga tcatcatcgg tgacggtcag aaccatccgc
     9301 ccgttggcgg tgctgacgcg gatatcgcgc ttattggtca gccctttcag gcgcacaaaa
     9361 cgggcaaagc agcgcgcacc gttgccgcac tgcgccactt cactgccatc agcattgaaa
     9421 atgcgatagt gaaaatccag ttcaggatca tacggcggct caaccaccag cagctggtca
     9481 aaccctaccc ccaggtgccg atacgccagg cgacgaatca gctccggtga aaaaaagaca
     9541 ttctgcgtta ccgcgtcgac gaccataaaa tcgttgccaa ggccatgcat tttcgagaac
     9601 tgcatcattt actccaatca cgcgggtaca gaaactgact tttagtaatt cacctgggat
     9661 ggaccatcgc cagtggcgcg atcgttttta tccggcaccg tggattgcgt ttgcgtctct
     9721 accggtttgg tcggcggcgg tgcgttttta tctgcaggcg ggaaatagag cggacctttc
     9781 agaccgcagc ccgtcaggct gaagagagta agtaatacag tgagtgcctt aaacacgttt
     9841 ttcattatag attgcctgta aagttcatcg ctttctgctt tctatcatcg caggagaagg
     9901 cgtaaaagca agcgtttagc gactctttat gaagtctgaa aagtgaggaa atgctgcgag
     9961 tataacgctt actcgtgggg atttatgttt tgctggtagt ttcgtgatcg gcacctcgcg
    10021 ccaggatggc tttatgcact atcgcaatgt ggtttgtttg ttggcttttt tagttccgtc
    10081 agcgtgagga tgctcgctcg gtaaacctga tcataatgtt taccagaaac agggaaaggc
    10141 gtggtgtaac tgcgccctaa tgaagagaca attctttcgc tacacagaga catcattatt
    10201 ccggtgacgg atgaatcatg ccgtaacgca gccgcagagg tgcaaacttt gaagccctga
    10261 ccccatgccg ctatactgcc gccatcactt aaaaacagga tacaacaatg aacgacagtg
    10321 aatttcatcg cctggctgat caactgtggc tgaccattga agagcgtctg gacgactggg
    10381 atggcgacag cgatatcgac tgcgaaatca acggcggtgt actgaccatt acctttgaaa
    10441 acggcagcaa aatcattatc aaccgccagg aaccgctgca ccaggtatgg ctggcaacca
    10501 aacaaggcgg ctaccatttt gatctgaaag gcgatgagtg gatttgcgat cgcagcggcg
    10561 aaaccttctg ggatttgctg gaacaggcgg cgacgcagca ggcgggtgaa acagtcagat
    10621 tccgctaaga ttgcatgccg gataagcctc gctttccggc acgttcatca cgaaaaatac
    10681 tgctgtaata gcggcgtatc gtgatcctga ttggcaggcg gcatgttacc gatagatttt
    10741 gtgcggaacg gaatcacctg ttcacgacca tcaaccttca caatctgata gaactgcggc
    10801 aggttgaagt tgatgaagct tgagccgtag gtaaaacggt catgcgatga cgagtagaag
    10861 cgactgacgt cacgtaccag ctcctctttg ctgccttcgc agtggtgata cacctcaacc
    10921 cggttgcttt cgtcgagaat gtagatatta aagccattct catcttgcgt ttcttcgaaa
    10981 aagaactgga tgatcccttc gctggcaaag ccgtccacca ccgccggtaa tttgacgtga
    11041 ttggtttcaa cctgcactga caggccgtgc agtttgttat gcgaaatagc gccataaaac
    11101 tcgatggcgt tctccagttt ctgtaccgat acattcaggc gttcgaagaa caagccccag
    11161 gtttgaccag aaacgcgcag cgccttaaaa cgcccggttt cctggcgggt gctggaaaga
    11221 cgcaattcaa tacactcaga aaccagttgc tgcacgcgag tacgaattaa gccgcgcaga
    11281 tgctggctat aacagaagac ttccacgcta tctggcggtg cggcgtcctg atgcattttg
    11341 ccgagaatag ttttcagggc ttcgatcatc gattgctcgc cgttgaagtg cagcgtacgc
    11401 acttcgttcc acgagttgcg gtacagcagg tcaacgctac ctaccaggca attttgattc
    11461 tcgccaaagc tgaagacatc cagcttacgg aaatcgaaat gcaccacctg attgcggaac
    11521 gccgctgtcg ggtcatattc caggttaacg ataatcgcca gatggcggat ctcacacggg
    11581 ctatagagcg ccttcggtgt cggtgcaggt aagcgcagcg ggaaatggtg cgacacgtcg
    11641 gcgaccatct cctgcaactt aggcaaatcg acaatgccgt tacctttaat atacaaacgg
    11701 gtgcgcgagg tcagcaggcc attaaaccat gcccacgcca ccagcttatt caggtaacgg
    11761 ttatattcca gcggctgatg gctgatgatc gactcaatat ttggcgcgcg gttatacaga
    11821 taccaacctg aacggttagc ccggtccggc ggcacataaa taaaggtcag attcggttcc
    11881 gagagatcgg gtgaaatctg cgggtttacc agcgtcactt tacctggtaa tgcttcaaac
    11941 gcggcataca gcttacgcgt cagcacgccg atatcctgcg gactggcgga gacgctaagg
    12001 ttattgcgac gcgcaaagcg gatcagatta cggtagctct gcatcatcgc gtcgagcaac
    12061 tcgttgtgcg cctcacgcac ctgatcaatc ttccagttag cgcggttatc gagcattgcc
    12121 agacgagctt cgtcccaacc ccactcgctc actaactggc tcaacactgc gcgacgccag
    12181 cctacgcagg cgcgttcacg gctgagcttt tcgcacactt ttaaatagaa gcagcgacgt
    12241 actaaatcca gacgggtaaa atcttcaatc gccgtcaggt attcagtaac acgctccagc
    12301 atcatgcagt atggatcgag accaaacgat acaatctcgc cgtcgtgcaa acgctgtttg
    12361 atatctttcg ccagcagacg tgggttcggg tattcccagg aataggcttc cagcagcagt
    12421 gttttcagta ccgctttgta tggggaatcg atactcttgt agagctgcca aaggctggca
    12481 ccaaagtact cttcagcaga aagcgagctt aagccaccga gatccagcca ttcatttggc
    12541 gtcagcacgc cttgtgcgta gagcgtcatt acgtagtcat cgtaatgctc ttcttcgtcg
    12601 cacggcacca tattccacag aatacgctta ccggcgagac gcacggcggt acgataaaat
    12661 tcatcaagca gcagtatatg ctgggtggag ccacaatctt cgccccccag actgccactc
    12721 tcgttgtggc gaaaacggtt ttcatcaatc aggaagaagc taacttccac acccagcgag
    12781 gcggcccagc tttccagcag gctgcattta cgttgtagca actggcgctc ttcgctatcg
    12841 agccaggatt gatgacagac ccagatatcc aggtcagagg aacaactttg ccctacggac
    12901 gaggtgctgc ccatggtgta tacaccagta attggaagct cacctttcgg cgggtcctgt
    12961 actgacattc cacgatacag ttcaagctcg ttcaggtagt ggcgttgagt ttcatcaggc
    13021 gtgtaaaggc aaatgccttt gggaacgtta ccatcaaggt aacccggcat tagcggatgg
    13081 tgatagtgca acaatgtcgg cagtagactg tagacctgtt ggaatgcagg ccccatagca
    13141 gcaagcgcgc gatccacacg caattgattt atggcatcca gtctctgttt cagagtctca
    13201 atatagaggt acaagacgta tcgcctgatt tgctacccgt catgactgtg attccgccaa
    13261 catcaacggt aacacgcggc attcgggata tttcgtatgt caaaggtaac cgttaccact
    13321 tttcgcgcct ggttttttta gtttcacgac gaaaaaatgg tctaaaacgt gatcaattta
    13381 acaccttgct gattgaccgt aaagaaagat gcgctacata caagtgtagc accgtttatt
    13441 ctctgtaaat tccttattac gacggcgtga aacgcctgtc aggatccact gccagacctc
    13501 attttacggt ttgcgcaggc gtctacgttt caccacaaca ctgacatcac tctggcaagg
    13561 atgttaggat ggaccacgga tgataatgac ggtaacaagc atgttagaca atgttttaag
    13621 aattgccaca cgccaaagcc cacttgcact ctggcaggca cactatgtca aagacaagtt
    13681 gatggcgagc catccgggcc tggtcgttga actggtaccg atggtgacgc gcggcgatgt
    13741 gattcttgat acgccgctgg cgaaagtcgg cggaaaaggc ttatttgtaa aagagctgga
    13801 agtcgcgctc ctcgaaaatc gcgccgatat cgccgtacat tcaatgaaag atgtgccggt
    13861 tgaattcccg caaggtctgg ggctggtcac tatttgcgag cgtgaagatc ctcgcgatgc
    13921 ctttgtgtcc aataactatg acagtctgga tgcgttaccg gcaggcagta tcgtcgggac
    13981 gtccagttta cgtcgccagt gccaactggc tgaacgccgc ccggatctga ttatccgctc
    14041 cctgcgcggc aacgtcggca ctcgcctgag caaactggat aacggcgaat acgatgccat
    14101 cattcttgcc gtagccggac taaaacgttt aggtctggag tcccgcattc gcgccgcgtt
    14161 gccacccgag atttctcttc cggcggtagg acaaggtgca gtcggtattg aatgccgcct
    14221 tgatgataca cgcactcgcg agctgcttgc cgcgctgaat caccacgaaa ctgcactgcg
    14281 cgttaccgca gaacgcgcca tgaatacccg tctcgaaggc ggatgtcagg tgccaattgg
    14341 tagctacgcc gagcttattg atggcgaaat ctggctgcgt gcgctggtcg gcgcgccgga
    14401 cggttcgcag attattcgcg gtgaacgccg cggtgcgccg caaaatgccg aacaaatggg
    14461 gatttcgctg gcagaagagc tactgaataa cggcgcgcgc gagatcctcg ctgaagtcta
    14521 taacggagac gccccggcat gagtatcctt gtcacccgcc cgtctcccgc tggagaagag
    14581 ttagtgagcc gtctgcgcac actggggcag gtggcctggc attttccgct gattgagttt
    14641 tctccgggtc gacaattacc gcaacttgct gatcaactgg cagcgctggg ggagagcgat
    14701 ctgttgtttg ccctctcgca acacgcggtt gcttttgccc aatcacagct gcatcagcaa
    14761 gatcgtaaat ggccccgact acctacttat ttcgccattg gacgcaccac cgcactggcg
    14821 ctacataccg taagcggaca gaagattctc tacccacagg atcgggaaat cagcgaagtc
    14881 ttgctacaat tacctgaatt acaaaatatt gcgggcaaac gtgcgctgat attacgtggc
    14941 aatggcggtc gtgagctaat tggggatacc ctgacggcgc gcggtgctga ggtcactttt
    15001 tgtgaatgtt atcaacgatg cgcaatccat tacgatggtg cagaagaagc gatgcgctgg
    15061 caatcccgcg aggtgacgac ggtcgttgtt accagcggtg aaatgttgca gcaactctgg
    15121 tcgctgatcc cacaatggta tcgtgagcac tggttactac actgtcgact attggtcgtc
    15181 agtgagcgtt tggcgaaact cgcccgggaa ctgggctggc aagacattaa ggtcgccgat
    15241 aacgctgaca acgatgcgct tttacgggca ttacaataac tctcataaca ggaagccata
    15301 atgacggaac aagaaaaaac ctccgccgtg gttgaagaga ccagggaggc cgtggacacc
    15361 acgtcacaac ctgtcgcaac agaaaaaaag agtaagaaca ataccgcatt gattctcagt
    15421 gcggtggcta tcgctattgc tctggcggca ggcgtcggtt tgtatggctg gggtaaacaa
    15481 caggccgtca atcagaccgc caccagcgat gccctggcta accaactgac ggcattgcaa
    15541 aaagcccagg agagccaaaa agccgagctg gaaggcatta ttaagcaaca agctgcacaa
    15601 cttaagcagg cgaatcgtca gcaagaaacg ctggcaaaac agttggatga agtccaacaa
    15661 aaggtcgcca ccatttccgg cagcgatgct aaaacctggc tgctggctca ggccgatttt
    15721 ctggtgaaac tcgccggacg gaagctgtgg agcgatcagg acgtcacgac cgctgcagcg
    15781 ttgctgaaaa gtgcagacgc cagcctggcg gatatgaatg acccgagtct gattaccgtt
    15841 cgtcgggcaa ttaccgatga tatcgccagc ctttctgcag tatcgcaggt ggattatgac
    15901 ggcatcatcc ttaagcttaa tcagctttca aatcaggtag ataacctgcg tctggccgat
    15961 aatgacagcg atggttcgcc gatggattcc gacggtgaag agctttccag ttccatcagc
    16021 gaatggcgta tcaatctgca aaaaagctgg cagaacttta tgaacaactt cattacgatt
    16081 cgccgtcgtg atgacaccgc cgtaccgctg ttagcgccaa atcaggatat ctatctgcgc
    16141 gaaaatattc gctctcgcct gctggtcgca gcacaagctg taccgcgtca ccagcaagag
    16201 acttatcgcc aggcgctgga gaacgtctcc acctgggtac gtgcttacta cgatactgat
    16261 gatgccacca ccaaagcgtt cctcgacgag gtggaccagt taagccagca aaatatctcg
    16321 atggatcttc cggaaaccct gcaaagccag gcgatgctgg aaaaactgat gcagactcgc
    16381 gtgcgtaacc tgctggcaca accggcagcg gggacaacgg aagctaaacc tgcacctgca
    16441 ccgcaagctg caccgcaagc tgcaccgcaa gctgatactc cggcagccgc gccgcaagga
    16501 gaataaccat gctaaaagtg ttattgctct ttgtgttgct gattgcgggg atcgtggttg
    16561 gcccgatgat tgccggtcat cagggttatg tgctgatcca gaccgacaac tacaatatcg
    16621 aaaccagcgt cacgggcctg gcgatcatat tgattctggc gatggtagtg ctgtttgcca
    16681 ttgagtggct actgcggcgg atcttccgca ctggcgcgca cacccgtggg tggtttgtcg
    16741 ggcgtaagcg tcgccgtgca cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag
    16801 gcgattatca gcaagttgaa aagctgatgg cgaaaaatgc cgatcacgct gaacaaccgg
    16861 tggtgaacta tctactggct gccgaagccg cgcaacaacg tggtgatgaa gcgcgcgcca
    16921 accaacatct ggaacgcgca gcggagctgg ccggcaatga caccattccg gtagaaatca
    16981 cccgtgtacg tctgcaactg gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata
    17041 agctgctgga agttacgcca cgccatccgg aagtattacg tctggcggaa caggcgtata
    17101 tccgcacagg tgcatggagt tcgctgctgg atattatccc atcaatggcg aaagcccatg
    17161 ttggtgatga agaacatcgt gcaatgctgg aacaacaggc atggattggc ctgatggatc
    17221 aggcgcgtgc cgataacggt agcgaaggtt tgcgtaactg gtggaaaaac caaagccgga
    17281 aaacgcgtca tcaggtagcg ttgcaggtgg caatggcgga acatcttatt gaatgtgacg
    17341 atcatgatac tgcccagcaa attatcatcg atggcctgaa acgccagtac gacgatcgcc
    17401 tactgctgcc gattcctcga ctgaaaacaa acaatccgga acagctggaa aaagtgctgc
    17461 gccagcaaat caaaaacgtc ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac
    17521 tgatgaagca cggagaatgg caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac
    17581 gtccggacgc ctacgattac gcatggcttg ccgacgcgct ggacagactg cacaagccgg
    17641 aagaagctgc agcgatgcgt cgcgacggtt tgatgttaac gttgcagaac aacccaccac
    17701 agtagttcct tctcacccgg aggcaagcac ctccggggcc ttcctgacac ataaaaaaac
    17761 gcctgctctt attacggagc aggcgttaaa acaggtctgt atgacaacaa gtgggtgctt
    17821 cactcaacgt tgtgtccatg gtgtctgatg aggcataagc gacatctgtc agtggacgat
    17881 aagcaccgta aacggctctg cgtcattccg gagtttatga ggcactaagg cgaacataag
    17941 agatggaatg agcatctact cgtttattat gccacagaga atcgggaaat aacatccctt
    18001 aacacttgtt atgagataat tctgtaatcc tcttggcttc ctgagtaata acttcctgag
    18061 tgaatattta ccctgagctt gattctacac atactcatta tgaatgataa aattcattca
    18121 attaataaca catatattaa ttgccgttaa aactaaaaac agcatcaata atcaacgcga
    18181 tataataaac ctgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg
    18241 attttccaat tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta
    18301 atcatcctgt cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat
    18361 gacatgaaac cggagcattt tgcatggaat tttcgttttc acccaaacgt cttgttgttg
    18421 ctgtcgccgc cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg
    18481 cacgcaaagg ctttgccgga tacgatcacc caaaccagta tctggttaaa ccggcgacca
    18541 ctattgccga caacatgatg ccagtaatgc agcatccggc gcaggataaa gaaacccagc
    18601 agaagctggc agaacttgag aaaaaaaccg gtaagaaacc gaatgtggtt gttttcttgc
    18661 tggacgatgt gggctggatg gacgtcggtt ttaacggtgg cggcgtggcg gtgggtaacc
    18721 ctacaccaga tatcgacgcc gttgccagcc aggggctgat tttaacttcg gcgtattctc
    18781 aaccaagctc ttccccaacc cgcgccacca ttctcaccgg acaatactcc atccaccacg
    18841 gcattctgat gccgccaatg tacgggcaac cgggcgggct gcaagggtta accacgctgc
    18901 cacagttgct gcacgatcag ggctacgtca ctcaggccat cgggaaatgg catatggggg
    18961 aaaacaaaga gtcgcagccg cagaacgttg gctttgatga tttccgtggc tttaactcgg
    19021 tgtctgatat gtacaccgaa tggcgcgacg ttcacgtcaa tccggaagtg gccctgagtc
    19081 cggaccgttc tgaatacatc aagcaattac cgttcagcaa agatgacgtt catgcggtgc
    19141 gcggcggcga acaacaggcc attgccgata ttacgccgaa atatatggaa gatctggatc
    19201 aacgctggat ggactatggc gttaagttcc tcgacaagat ggcgaagagt gataagcctt
    19261 tcttcctcta ctacggcact cgtggctgcc acttcgataa ctacccaaat gcgaaatatg
    19321 caggtagctc tccggcacgc acctcgtatg gcgactgcat ggtggaaatg aacgatgtgt
    19381 tcgctaatct gtataaagca ctggagaaaa acggtcagct tgataacacg ctgattgtct
    19441 ttacctccga taacggaccg gaagccgaag taccgccgca cggacgcaca ccgttccgtg
    19501 gtgcgaaagg atctacctgg gaaggcggcg ttcgcgtacc gactttcgtg tactggaaag
    19561 gcatgatcca accgcgtaaa tctgacggta ttgtcgatct ggcagatctc ttccctaccg
    19621 cgctggatct ggcagggcat cctggcgcga aagtggcgaa tttagtgccg aaaaccacct
    19681 ttatcgatgg tgtggaccag acatctttct tcctgggaac aaatggtcag tctaaccgta
    19741 aggccgagca ctacttcctc aacggtaaac tcgctgctgt gcgtatggat gaattcaagt
    19801 atcacgtcct gattcagcaa ccttacgctt atacccagag cggatatcag ggtggattca
    19861 ccggcacagt aatgcaaacg gcgggatcgt cggtgtttaa cctgtacacc gatccgcagg
    19921 aaagcgactc cattggcgtg cgccatattc cgatgggtgt accgctacag accgaaatgc
    19981 acgcgtatat ggagatcctg aaaaagtatc caccacgcgc gcagattaaa tctgattaag
    20041 ccggcgctat cgctgagatc tgcctttgcc ggatgcgatg ctgacgcatc ttatccagcc
    20101 tacagaacgc tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg
    20161 ccgcatccgg caatcaaccg caggcggcca caaaatggcc gcttttttac ttactcacca
    20221 ccagcaaatg cgcatgcata atgtcgctgg ccggacgacc gtgcgccagc aaatcagcca
    20281 tagctttaag atatggcggt agatggcgga aataacgctg atacccggca cacaaataat
    20341 tcagccccgg tttgccgctg gcatcgagca tgaagcggtg tttcggacag cctccccaac
    20401 atgcttttaa cacgttacaa ctgcgacact gcgccggtaa ctgcttaaat ttatcttcac
    20461 caaaagcctg ctgctgcgga gaatctacca tttctgcaat tgtttgctgg tgcatattcc
    20521 ccagtcgata ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa
    20581 caatcaccga gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa
    20641 aattggcaaa cgcccattcg atattcatca caaaaatctt gccgacatca cgtttgatcc
    20701 agtggtcgaa aatcgccacc agaaattcac caaattcatc ggggcgcacc gaccattccg
    20761 ttagctcacc ctgaatatca ccaggcgcat gcaatttcag tccttcgcgg gcagccgttt
    20821 catcagccaa gcgctcgacc accggaataa actggatgaa ttccactccc gcatcgcaca
    20881 aaaaatcata tacctgcaac ggttgctgcg cgctggtgcg attaacgcag accagcacgt
    20941 tatagtcgac atgatgtttt tgcagcagcg tcagggcacg catcaccagc ttatgcgttg
    21001 gtctgccacc tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata
    21061 acccaacaag aaaatgattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc
    21121 cgttagtctg gaagctgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcta
    21181 cggcacggcg gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag
    21241 ccacttcgtt ttgcggttcg ctggcagcga tatagtaacg gacatacgct tccagcgtgt
    21301 catcgtccat atgcgtgact ggtttttcgc ggtaaaggga ttgtttttcg agataaaaac
    21361 agtagtcaca gttcagatta caatcggaac cgctcggttt cgccatcaca tgaaaagcac
    21421 gcgttggaac ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa
    21481 gggcgaaatc ataccgtggg gcaatgagtt attacgggcg tttattgcga aggaagggtg
    21541 atagaatgca cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga
    21601 gaggattcga acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact
    21661 cgccgaaata ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg
    21721 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
    21781 tccgccacct tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc
    21841 acgacaactg gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat
    21901 cttgttacgc ggtattacta ccaccgcagc tcaagcgccg ggactaaatg gcgcgcccga
    21961 caggattcga acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg
    22021 ggcgcttagc gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc
    22081 tttttaaata aattgcgcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt
    22141 ttatgcgctt tcccgtggcg attaaggcca aaaactttat aaatcgccgt caccgccagc
    22201 ataaagatga tgccaacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc
    22261 aaaacgcaaa tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg
    22321 aacggatggc tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat
    22381 atcacaaacc atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca
    22441 cgctgcggat tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat
    22501 actgccacac ccgcaacggg tacgccgtga cgggaaactt tcgccatcgc cgccggtaac
    22561 tgacggtttt tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag
    22621 ccagagagcg cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg
    22681 attttggcaa aagtcagtac gaacgggctg ccgttgctgc ctatttcatt ccacgggaag
    22741 atggtgacga taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcaccttg
    22801 cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc
    22861 agctccacgc cctggtagga cgccaccaca atacacagcg cggtcaggaa ccctttccag
    22921 ccacccgcaa agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca
    22981 ttgccaaagc caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg
    23041 actttgatca tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc cgccagattc
    23101 gccaacgcca ccagcgccac tgcgatcaat gcgggtatcc actgcgccat ctccgggaac
    23161 cagaactgga cataaacgcc aatggcggtg atttcagaga tccccaccgc catccacata
    23221 aaccagtaag accaggcggt gagatagcca aagaacgggc tcatataacg atgcgcataa
    23281 acggcgaacg aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg
    23341 aaaaagacga acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc
    23401 agggtactgg cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt
    23461 tcgatatgtc gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct
    23521 cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt
    23581 gcgaatgttt tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt
    23641 gtgtaataaa aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga
    23701 aaatcagagg ttgccggtgt aatgccagcg taaataacgc agcaaacgaa gctgacgctt
    23761 aatgcggctc ggctgcgaaa gcaggcggta gagccactcc agccccagcg tttgccagat
    23821 tttcggtgcg cgtttcacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata
    23881 cagcgcatct ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc
    23941 catcgcaacg gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg
    24001 ctgctcgggt ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg
    24061 cagtttagct tcagtttgcg ccagcacttc aggtttaccg cccacaagaa atactggcgt
    24121 cccttctttg cctgcgcgcg ccatcagctc ttcccaaaga tcggcaccgg caacgcggga
    24181 aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata
    24241 tttaaattcg gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat
    24301 tttttcggca ttaatggcaa ccaacgttcc ctgcttaagc tgcccgtcag caaacagata
    24361 atcgagggcg tgctgcatat cacgccaacc gatcaactgt aagccacgca gcgtataggt
    24421 cggtgccgtg gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt
    24481 gtacgtttat gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc
    24541 agacatgcgc cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct
    24601 tcacgcgcca gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca
    24661 gccttatagc gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg
    24721 atcagtccaa ccacaatcgc cccgagcggg atgaacaacg cgccgcccat caccaccagt
    24781 gagcctataa gcgtaggcga gatcgccagt ccggagtggt tattcagcac ttcccaggta
    24841 aagtagttgg ctgagttcag caccatactc gggcgacccg gccacagcca ggaagggata
    24901 aagacataga aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc
    24961 tgcaacagca acgccagatt ctcccacggc gagaaggtgt cgcgagtgag atagagaaac
    25021 gtatagaacg cttcatcgcc gctcacattc attccatagc gttttagcgc cagccagaac
    25081 atgccaacaa tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga
    25141 ataatgccaa taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg
    25201 acaatcatat aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct
    25261 ttgctgtcct ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag
    25321 cgttttaacg ccacgccgga gacttcactg gagaagatct gactgtagga gttaagccgg
    25381 aacagcaaaa agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatgcctatc
    25441 aggatcaccc acgtaagatt ggtttccacg cggttcatgg taaacagcgg acggcgcggt
    25501 acatcagcaa cgcgtttgcg taggcgggtt ttgtaggtga cgtagtaaac cgcgtagaag
    25561 cagcccgcag aaagcaacgc ctgcaacaag atttctggcg gcgcgacacc aacatcaaag
    25621 cgaaatacca gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat
    25681 gaaaagaaga cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca
    25741 ataaacagcg tgcagagcag ccagacaaca aacaggccac tgaattgcag cagactcatg
    25801 cgacctcccc ggcggcaatc gccaacgccc gctgccagcc ttgcagatag ttcgggctaa
    25861 agaaggcaat ggtgttttta tccaccgacg ccaactggcg ctgcgcttca cgcacaatat
    25921 cctcgttgag atcgtcggta gtaaacagca ccggcaaatg ctgttccgtc atatcctgcc
    25981 agaacggatt ttcccggtta agcacacaag gaatgcccgc ctgaatcagt aagcacagcg
    26041 taccaatgcc ctgctggcgg gcaaaaataa agtaaccgag atcgcactga cgaagtagcg
    26101 ccagataggc gtcaaattcc agtttttcgc tcagaacttg cagattttct tcgctgaata
    26161 actccagccc agcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca
    26221 tcggcaccac cacttttacc gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa
    26281 tatgctcatt gctgcggtcg ccggagttcc ccaccagaat ggtcattttc ccttcacgtt
    26341 gccgatcgtt cgccatcgta ttgagagaag agtccatccg cgtcgggaag tacagcagtt
    26401 cgccccgcac ctttggatgc gttttggcaa aaaagctcaa atcaccgcgg gtggcaaata
    26461 cacagccgac tcgcttttgc gccaggcgac gtagcgggta aaaaagctta tatctcaagc
    26521 cactggaaag ctcatacaga tctgcccccc agatatgcca gaaaaactgg cgggacttaa
    26581 tgccaccact cagcaaagcc agccacagtt tgggattgaa ctgaccgtgg aagaaaaaac
    26641 gctgctgacg gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag
    26701 ggaaaaattg cacagaaagc gccggacagt tatcacttaa gccgtcgtcc ttgccaacaa
    26761 ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa
    26821 cggttcggtt atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg
    26881 cctacgccag agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat
    26941 ataggcctgc gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat
    27001 caataaagtg aactggctga cttccgccag aatataaaac cgcagtgaag ctttggcgat
    27061 caccagataa ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc
    27121 aaagagatcg cgcatagcgg taaatttatt cgacaacagc agccagatag caaaatcacg
    27181 cagcaaccag acggtaaaac tcgccgccgc cactgccggt aagacgaatt tcagcgattt
    27241 aaccacttcc cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata
    27301 tacgctgaac gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg
    27361 ccagatcccc acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta
    27421 agcaacaggc aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc
    27481 tgccagaccg ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttgat
    27541 caacatgatg gcggcaggaa tcaccaccag cgcgggaatc agcgccagac ccagcaacgc
    27601 cccttcataa ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc
    27661 aacaatcagc gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa
    27721 caggttgccc caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata
    27781 gtcggtatta ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc
    27841 catcagcgta gagaagccaa ttaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg
    27901 ttggggatta tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc
    27961 ggcaagcacg ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc
    28021 cggaccaaat gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag
    28081 tgtactggcc gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca
    28141 acaaagtcgc aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc
    28201 gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagc
    28261 gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa caccgccatg atttccgctt
    28321 ctttcagaaa gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca
    28381 tatgcgcgtt ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg
    28441 ccagaggtgc taacgcatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac
    28501 gatccgctgc ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc
    28561 tggagccaat atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt
    28621 tagtgccctt ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg
    28681 tcgcgccgcc ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa
    28741 tatgaccaat ggttccaagc gcacgccctt tgtaagtgga catcacgccc tgagcggcat
    28801 cttctaccac aaacagatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg
    28861 ccacacccgc gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa
    28921 tcagcgtttc gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc
    28981 cacgcagcac aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat
    29041 cgccaggctg gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg
    29101 tcagtaacac tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa
    29161 aaccgccatc gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg
    29221 ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg
    29281 gtgctttcta cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt
    29341 tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc
    29401 atcagctcag cacctgcacc gcgtccagcc agcaggccta ttcgcgcatc tgtcgcattg
    29461 agttcccgta aagagacata gccgcgaata tcgccggacg ccgcacgtaa aatcagacat
    29521 tgatgatcaa aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgactgctg
    29581 gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctggctaac
    29641 tgacgtaatg cgggaatatc ggtctcttgt gccactacag caccgctatc actggcattg
    29701 ttcacgggta gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg
    29761 gcatccagtt cacccgtatt tgatgccgca attttggcct gcacccgtga ccacggcgct
    29821 aacgcgtctg gcgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca
    29881 ccaaagaagg cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa
    29941 ctccagcaga tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc
    30001 ctcgtcatcg agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc
    30061 ctggcgtttt tccaccgtct gtacaaaggt gctggcttca atcaggctgt cgtgagtgcc
    30121 agtgtccagc caagcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag
    30181 gtacatctgg ttgatggagg taatctccag ttcaccacgc tccgacggct tcacctgctt
    30241 tgcgtactcc acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt
    30301 cggctgtttt ggcttttctt ccagcgagat agcgcggaaa ttgtcgtcaa actccaccac
    30361 gccaaagcgt tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc
    30421 cgcaacatga cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac
    30481 cagacaagaa ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc
    30541 gtccgggctg ggctgttcgg catattccag ctggataccg aactcactac catcgcccag
    30601 caggcgctgg aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc
    30661 ggccagcatc agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag
    30721 ttgcttcgat acgccgcgcg taatcggatg caatcgggtg ccggaaccgc ccgccaggat
    30781 aatacctttc atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcgccctg
    30841 atagctgccg tcctgcacct gcttccacca actttcatta gccagatacc actgcaccgt
    30901 tttacgcatt ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt
    30961 cgaagcatca atggcatagc gcagatcatg ccccggacgg tcagcgacaa aggtgatcaa
    31021 gtcacgataa tgcaccacgc cttgcggctt gttcggagcc agttcttcca gcagctcgca
    31081 aatggtttcc acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc
    31141 accgactttc ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag
    31201 ccagtcacgg atttgctgcc cgttgccata taccggcagc gatttacccg ccagcgcgtt
    31261 gaggatcatc agcgggatca gtttttccgg aaagtggtaa gggccgtagt tattcgagca
    31321 gttggtgata agcgtcggca gaccataggt ccgcagccag gcgcgcacca gatggtcgct
    31381 gctggctttt gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa
    31441 atcatccgtc gagtgcaggt caccatatac ttcgtcagtg gagatatgat gaaaacggaa
    31501 cgctgatttt ttatcttccg ttagcgcatt ccagtaagcc cgcgccgctt caagcaatgt
    31561 ataagtcccg acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac
    31621 atggctttcg gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgcgc
    31681 cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac
    31741 cggtgccagc gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcgtc
    31801 gctcgtttcg ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt
    31861 tatcagaatc tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg
    31921 acattgtcgc cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct
    31981 gccgttgcca gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc
    32041 aactggtgga tgttaggctc aacaaccaga gtttcgccgc tatgccactg ggcgatcagt
    32101 tcagcgattt ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga
    32161 ccaaagcagg cgattttcag ttcactggcg cgtttatcgg tagccgccag gcaatcagcc
    32221 accgccgctt tcacctgatc gataacccag aacggtttgt gatcgttcac ttcgcgcgcg
    32281 gtacggataa gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca
    32341 atgcagtgac cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc
    32401 gccaggcgaa tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca
    32461 ttagcaaaag cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc
    32521 gtccgcgagt tagtgacgac acactcacct tcgaggaaaa ttttgtacag ttcgctggcg
    32581 cgggccgaac aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc
    32641 ataacctggc ccggtaacac gcgttccggg cagtaagcaa tgttgacgtc cgcctgctcg
    32701 cccacctgct gtgggaaagt gagatccgga cgcatctctg ctaaccattc tgccatcttc
    32761 tcggttgacc ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact
    32821 ggcgcaatgg agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta
    32881 aacggcgtgg gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt
    32941 aaaaaaccgc cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg
    33001 atttcgccac gattgatagt atcaaccgcg tgttggttga tatcgacacc aatcacctgt
    33061 ttttgccgtg aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca
    33121 gaaatggtcg caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag
    33181 agcatgcctg accatcacca tacgggttat gggcgcggct catagtttga tattcgtttt
    33241 cgtcttttaa aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac
    33301 gcaccgtacc cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg
    33361 gtttccccag cgaaggcgct tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg
    33421 cgtggttcat cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt
    33481 tcacatgccc cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat
    33541 agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt
    33601 caaagccacg accgaagctt tcacgcctgt gaccggtcac cagaatcatc tttttatcgg
    33661 ggtcgataaa cgggtaattt gccgcaagtt ctgaacgcag cgtgtcgctg ctcatcacct
    33721 ggtcacgcac ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat
    33781 ctgcaacgtt ttcacgcagc aagttttgcc gggaagtttc ggttggagag aaatgataca
    33841 tcgccagatg cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagatcgc
    33901 ccgtgcgtaa accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc
    33961 tggttgccag cgtcgtcgtt gtgtcgccgt gaaccagcac gacgtctggt ttgaactcgg
    34021 caagaatagg ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg
    34081 gctgcattat gttgagatcg tagtcaggta caatggaaaa gagtttcagc acctgatcga
    34141 gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca
    34201 acgcatgcac caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta
    34261 ctttcacatc gattctcttc gaataagcgg cgagcgactt tgcgctcacc gcagcagtgt
    34321 tgctatttcg agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg
    34381 ccccacataa tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc
    34441 gtacgcaaat agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg
    34501 tttaacatgg cccgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa
    34561 ttttccagtc gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat
    34621 tcctcggcag gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc
    34681 gcctgctcaa tgctgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc
    34741 ttcacctgag ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc
    34801 agatggctgg ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg
    34861 tcaggcgcgg tttcggcaat aagcttcacg ctgtcattga ccgcgcgggt aaagtctccg
    34921 gggataaact ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg
    34981 cccaccatcc gctgcttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac
    35041 gaggccagtt gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttggtcggca
    35101 gaagccatgt ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc
    35161 cccagcatat tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca
    35221 aaaaacgtat acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc
    35281 ttcccagccc acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg
    35341 gccggtttcc caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc
    35401 tgccaccacg atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc
    35461 aggcacgctt aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga
    35521 cccactccgg gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg
    35581 cggcaagggt aatcagcaca aacgcctgac gggaagtaaa cccggcacgc atgatcaaat
    35641 ggtgaatatg ctgccggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca
    35701 taatcgccac catatccatt agcggaatgg cgattatcca caaagcggta accgggctga
    35761 tgggatgggt tttgccctgg gtcgtttcga gcaggatcca gatcacggta aaaccaatca
    35821 gcgtactgcc cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa
    35881 gcatgatgta tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt
    35941 gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga gacacaggac aacccgccca
    36001 gcaagccatc aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata
    36061 gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca
    36121 ggctactgag ataaagcttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg
    36181 tggcacggat ttttacgctg atatcaaaac ggtcatccag cgcgccaatg aaaacaagca
    36241 caccggcaca agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg
    36301 tgaagcaaat ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac
    36361 gtttgcggaa gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa
    36421 acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat
    36481 tcacaatgca tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt
    36541 cagaagggaa agtccgttcc gaacccacca gtcctgtgca aaaattaacc atttgatatg
    36601 caaattgcat aacatgccag caaattccag gaattttcct aacatcgcca gcgcggcatt
    36661 aagattacag atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca
    36721 aatttatgag cgtttcatca tttcgaagaa atcgtcattg gtcttggtca ttgccagttt
    36781 attaatgagg aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat
    36841 ccacattttc tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg
    36901 gttgtagtcg atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc
    36961 catgttgcct gtacctttaa actcttcgta gataacttcg tccattttag aaccagtatc
    37021 gataagcgcc gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc
    37081 aaagaagcgt ttcggacgat gaagggcatt ggcgtccaca ccaccggtca acactttacc
    37141 tgacgccgga acaacggtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat
    37201 gataacgtct ttcttgtgct caaccaggcg tttcgccttc tcgatcacca tttccgcaac
    37261 ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag
    37321 acgctgcatc tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca
    37381 atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc agcagcatgg ttttaccggc
    37441 tttcggcggt gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag
    37501 tacgcgagca gttaaatctt ccgtagaacc gttaccacgt tccatacgca gacgagagtt
    37561 tgcgtgcagc ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc
    37621 gaagttaact tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg
    37681 aatcttacca gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga
    37741 aacgtagatg tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa
    37801 tccatcctgc aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg
    37861 ctgcttcagg atggcaaaaa taatgtcctg cttacgcata cgagccaggt tttccagccc
    37921 catattttcg ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt
    37981 catagtggtg tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat
    38041 ggcaggatca tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac
    38101 aggagagtac gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc
    38161 acttcgcatt taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg
    38221 tatgtttaat acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta
    38281 gcgacggggc acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc
    38341 aactgacctt tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc
    38401 agcagagtcg ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg
    38461 ttcagttttg caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg
    38521 gcgatcattt tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc
    38581 gctttgagta catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata
    38641 tataactcca caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa
    38701 ctaaaggttg actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc
    38761 taccacacta tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat
    38821 ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca
    38881 ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc
    38941 gggaaaacga tggcgtttct tacgtcaacg tttcattatc ttctctctca tcctgcgatt
    39001 gccgatcgca aggtgaatca gccgcgtgcc ttaattatgg caccgacgcg tgaacttgcc
    39061 gtgcagatcc atgccgatgc ggaaccgctg gcggaagcta ccggcctgaa gctgggtctg
    39121 gcttacggtg gtgatggtta cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt
    39181 ctgattggca ccacgggtcg tttaattgac tacgccaagc agaaccacat taacctcggt
    39241 gccattcagg tagtggtact ggacgaagcc gatcgcatgt acgatctggg ctttattaaa
    39301 gatatccgct ggctgttccg ccgtatgccg cctgcaaacc agcgcctcaa catgctgttc
    39361 tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa
    39421 tatattgaag tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac
    39481 ccttctaacg aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac
    39541 agagcgatta ttttcgccaa caccaaacac cgttgtgaag agatctgggg ccacctggcg
    39601 gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt
    39661 attcttgatg aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg
    39721 cgtggtttgc atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt
    39781 gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatt
    39841 agcctggcgt gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac
    39901 tcaattccgg taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg
    39961 cgcctcacgc gcccgcgtac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt
    40021 cgtcgttcag gttaaataaa atgtcatact caaaatcatt caaactgtat caagatggca
    40081 agagaatgaa tcctgaatca cttactgtag taagtgattc aggtgaaaga atgcagccaa
    40141 cacagagaca gattgaagga tgaagagtat gggttccacc tcgtcgctgt atgcagccat
    40201 tgatctcggt tcgaatagtt ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca
    40261 gacgctgacg cgaataaaac gcaaagtgcg tctggctgct ggcctgaata gtgaaaatgc
    40321 cctctctaat gaagcaatgg agcgcggttg gcaatgtctg cgcctgtttg ctgaacgtct
    40381 gcaagatatt cctccctcgc aaattcgcgt tgtcgctacg gcgacgttac gccttgccgt
    40441 caatgcgggt gattttattg ccaaagcgca ggaaattctc ggttgtccgg tacaggtgat
    40501 cagcggtgaa gaggaagcac gtctgattta tcagggcgtt gctcacacca ctggtggtgc
    40561 cgatcagcgc ctggtggtgg atataggcgg agccagtact gaactggtaa ccggcacggg
    40621 tgcacaaact acctcgttgt tcagcctgtc gatgggctgc gtcacctggc tggaacgcta
    40681 ttttgccgat cgtaatctgg ggcaggaaaa ttttgatgct gcagaaaaag cggcacgcga
    40741 agtgttacgt ccggttgccg atgaattacg gtatcacggc tggaaagtgt gcgttggtgc
    40801 ttccggcacc gtgcaggcgt tacaggaaat catgatggca caggggatgg atgaacgcat
    40861 taccctggaa aagttgcagc aattgaaaca gcgagccatt cattgcggtc ggctggagga
    40921 actggagatt gacgggctga cgctggaacg tgcgttagtg ttcccgagtg gtctggcgat
    40981 cctgatcgcc atttttaccg aactgaatat tcagtgtatg accctggcgg gcggtgcgct
    41041 gcgtgaaggc ctggtctacg gcatgttgca tctggccgtc gagcaggata ttcgcagccg
    41101 tacgctgcgt aatattcaac gccgctttat gatcgatatt gatcaggcac agcgcgtagc
    41161 caaagttgcg gctaacttct tcgatcaggt ggaaaacgaa tggcatcttg aagcaataag
    41221 ccgcgatttg ctcatcagcg cctgtcagct tcatgaaatc ggcctgagcg ttgacttcaa
    41281 acaagcgccg caacacgctg cttatctggt gcgtaatctg gatcttcccg gttttacccc
    41341 cgcacagaaa aaattgctgg cgacgctact gctcaaccag actaatccgg tcgatctctc
    41401 atcgctgcat cagcaaaatg ccgtaccgcc gcgcgtcgca gaacaactct gccgtttact
    41461 acgcctggcc atcatttttg ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt
    41521 acaggctaac catgaactgt tgaccttgac gcttccgcaa ggttggctaa cccaacatcc
    41581 gctgggtaaa gagattattg ctcaggaaag ccagtggcag agctatgtcc actggccgct
    41641 ggaagtgcat taatgagtaa gtgccggatg cgatgctgac gcatcttttc cggccttgat
    41701 tatttccctc gttttgccgc catcatcgct ttcagattcg ccagatggct ttgccctttc
    41761 tgcatccgtt cttcggcgct gaccactttg cgctcctgtt cccaaatcag atcatcctgc
    41821 ggcagctcca gcaaaaagcg gctcggctcc gggcgcacca gttcgccgta ctgacggcgt
    41881 tctttacaca gcgtaaaggt caattccttc tgggcgcggg taatgccgac ataggccagc
    41941 cgccgctcct catcgatatt atcttcatcg atgctgctct ggtgcggcaa aaacccttct
    42001 tccataccga ccatgtagac ataaggaaac tccagccctt tcgacgcgtg gagagtcatc
    42061 agttgcacct gatccagctc ttcttcactc tcaccacgct ccatcatgtc gcgcaaagta
    42121 aagcgcgtca ccacctgggt taacgtcatc ggctcatcca gttcactgcc ttcgagcatc
    42181 tccgtcatcc agctaaacag ttggttgacg ttcttcatgc gcatttcggc ggctttcggg
    42241 ctgggcgatg tttcgtacag ccaggattca taatccatgc cgtggatcag atcacgcacc
    42301 gcggcaatcg gctcccgctc cgccagacgc tggatttctg ccaaccagtg agtgaagcgg
    42361 gtcaatgctt cataaccacg tccgctaagc gtctgactca ggcccatatc aaagctggcg
    42421 gtaaacatgc ttttattgcg cgtcatcgcc cattcaccca acttttttag cgtcgccggg
    42481 ccaatctctc gcttcggcgt gttaacgata cgcagaaatg cgctgtcatc gtccgggtta
    42541 gtcagcacgc gcagataagc cagcaagtcc ttgatttcag gacgagagaa aaacgacgta
    42601 ccaccagaaa ttttgtacgg gatgcggttt tgcatcagga atttttcaaa cacccgcgac
    42661 tgatggttac cgcgataaag aatggcgtaa tctttgtact gcgttttatt gacgaagtga
    42721 tgggcgatca gctcgccagt aacgcgctcg gcttcatgtt cctcgttatt cgcgcttaaa
    42781 acttttagct ctgtgccgta acccagttcg gagaacagac gcttttcaaa gacgtgcgga
    42841 ttattggcga tcaggatgtt cgccgctttc aaaatacgtc cggaagagcg gtagttctgc
    42901 tcaagcttaa tcaccttcag cgccggaaaa tcctgactca gcagcaccag gttttgtgga
    42961 cgtgcgccgc gccaggagta gatcgactgg tcgtcatcgc ccaccacggt aaagcgcgcg
    43021 cggctgccca ccagcagttt caccagctca tactggctgg tgttggtgtc ctgatactca
    43081 tccaccagca gatagcgaat tttgttctgc cagcgctcgc ggacttcttc attgcgttgc
    43141 agcagcaacg tcggcaataa aatcagatca tcgaagtcga gaacgttaca ggctttcagg
    43201 tgtgcatcat acagcccata acaatgggca aaaatacgat cccgctcgcc ctttgcctct
    43261 gccgccgcct gcgccggtgt tttgagatca ttcttccagt tagagatggt cgaaatcagt
    43321 tgttgcagga gaactttgtc atcttcaatc agcccctcgg tcaactcttt aagcaaagca
    43381 agctgatcgg tatcgtcaaa caacgagaag ttcgctttca tcccaagcgc cgcatactcg
    43441 cgtttgatga tatccagccc caacgtatgg aaagtggaga tcatcagccc acgcgcctct
    43501 ttgcgcccca gcgtctgccc tacacgctct ttcatctcgc gcgctgcttt attagtaaag
    43561 gtcaccgccg caatgtgccg cgcctgataa ccgcaaccgc ggatcagatg ggcgatttta
    43621 ttggtgataa cacgagtttt accggaaccc gcgcccgcca gcaccaggca ggggccggta
    43681 acgaattcga cagcttgttg ttggccgggg tttagacgca taggtgtatt gctcaatctt
    43741 cgaacggggg gaggattgta gcagaatcgc atttgaatgt gaccgcgtgt aagccgctaa
    43801 ccggttggct gcggatgttt aaatcagaga gagaagaccg aaatcggagt tcatcgtgaa
    43861 tccattagac ttagaaaata tcgggtcgta acgttgtgtt attacttttt cagagtttac
    43921 taaggagagg tttactcgtg gcgattaatt tcagccctaa ggttggtgaa atactggaat
    43981 gcaactttgg taactatcct gttagccaaa atggaccgtt catcaccacg tattacgatg
    44041 gccgcattcc tcctgaaatg ataaaaaaca ggttagttgt cgtgctcaac ggaaaaatta
    44101 atggcaacgc atgtatcgtc gttccattat caaccactcg cgaccatgac aagttaaatc
    44161 gtggaatgca tgttgaaata gccagtaata ttatcaatga cctgcaattc tttgatcagc
    44221 agattcgttg ggccaaagcc gacctggtac aacaagtcag tcgaaacaga ttaaacagag
    44281 caagaactta cagagggtat cttaatcaat gtttgccaca tgagttggtt gcagacatac
    44341 agagggcggt catcaagtca atcaatgcca tatcgctcat aaattgacaa taaaatcacc
    44401 atccccgata atgactctgt agcccataga ggccctaagt tttctcccct tgagggacct
    44461 gcgaagcccg gattttccgg gcttttgcca tatatgtgac ccgtcctgac tgacaattac
    44521 gttagcatct gcggatcatg ctaaaatcgc cgcctgacaa ttattcactc aaggcacgat
    44581 catggcaaaa acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga
    44641 tcttctcgag cagattaaga acggggccga ttttggcaag ctggcgaaga aacactccat
    44701 ttgcccatca ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc
    44761 ggcgttcgat aaagttgtgt tctcttgtcc ggtactggag ccgactggcc cgctgcatac
    44821 ccagttcggt taccacatca ttaaggtgct ttaccgtaac taacagtaag gccttctcca
    44881 ggagaaggcc ttgaactctg aatcgctatt aacccgcaac agcaatgcgt ttcatatctg
    44941 tcatatagcc gcgcagtttc ttacctacct gctcaatcgc atggctgcga atcgcttcgt
    45001 tcacatcacg cagttgcgcg ttatctaccg cgccttccgg aatagcttta cccaggtcgc
    45061 ccggttgcag ctctgccata aacggtttca gcaacggcac acaagcgtaa gagaacagat
    45121 agttaccgta ctcagcggta tcagagataa ccacgttcat ttcgtacaga cgcttacggg
    45181 cgatggtgtt ggcaatcagc ggcagctcgt gcagtgattc ataatatgca gactcttcaa
    45241 tgatgccgga atcgaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga
    45301 tcatcagtac gcctttatcg aagtattcct gctcgccgat tttgccttca tactgcggcg
    45361 cggtttcaaa cgcggttttg ccggtttctt cacgccaggt cagcagtttc ttatcatcgt
    45421 tggcccagtc cgccatcata ccggaagaga attcgccgga gatgatgtca tccatatgtt
    45481 tctggaacag cggtgccatg atctctttca gctgttcaga cagcgcgtaa gcacgcagtt
    45541 tcgccgggtt agagagacgg tccatcatca gggtgatgcc gccctgtttc agcgcttcgg
    45601 tgatggtttc ccagccgaac tgaatcagtt tttctgcgta tgccgggtcg gtgccttctt
    45661 ccaccagctt gtcgaagcac agcagcgagc cagcctgcaa cataccgcac agaatggttt
    45721 gctcgcccat caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac
    45781 ggtgaccgcc ggtcgctgcc gcccaggctt tggcaatcgc catgccttca ccttttggat
    45841 cgttttccgg gtgaacggca atcagcgtcg gtacaccgaa tccacgtttg tactcttcac
    45901 gtacttcggt gccagggcat ttcggcgcaa ccattactac ggtgatgtct ttacggatct
    45961 gctcgcccac ttctacgatg ttgaaaccat gcgagtaacc cagcgccgcg ccgtctttca
    46021 tcagtggctg tacggtgcgc actacatcag agtgctgctt gtccggcgtc aagttaacca
    46081 ccagatccgc ctgcgggatc agttcttcgt aagtaccaac tttaaagccg ttttcggtcg
    46141 ctttacgcca ggacgcgcgc ttctcggcaa tcgcttcctt acgcagcgcg taggagatat
    46201 ccagaccaga atcacgcatg ttcaggccct ggttgagacc ctgtgcgcca cagccgacga
    46261 tgaccacttt tttaccctga aggtagctcg cgccgtcagc gaattcgtcg cgacccataa
    46321 agcgacattt gcccagctgt gccagctgct ggcgcaggtt cagtgtattg aagtagttag
    46381 ccatggtgat tcctcgtgat gttgtgcttc ttattgttcg gttcgctttt gcgagattga
    46441 attcactata tgacaggaaa tttattgcgg aaattgatat attcacaacg tcacattgca
    46501 atttttgcaa cgtcaacatc gagggctgtc cctgtggatt tacgcgatct gaaaaccttc
    46561 ctgcatctgg cggaaagccg ccattttggc cgcagcgcgc gggcgatgca cgttagccca
    46621 tccacgctct cacggcagat tcagcgcctg gaagaagatc tcggtcagcc gctgtttgtg
    46681 cgcgataacc gcacggtgac gctgactgaa gcgggcgaag aactgcgcgt tttcgcccag
    46741 caaacgctgt tgcaatatca gcaattgcgc cacaccatcg accagcaagg accgtcgctc
    46801 tctggcgaat tacatatctt ctgctcggtg accgctgcct acagccatct gccgccgatt
    46861 ctggatcgct tccgcgcgga acatccgtcg gtggagatca aactcaccac tggtgatgcg
    46921 gcagatgcaa tggaaaaagt ggtcacggga gaagcggatc tggcgattgc cgggaaaccg
    46981 gagacgctac ccggcgctgt ggcgttttcg atgctggaga atctggcggt agtgctgatt
    47041 gcgcccgcgc tgccctgccc ggtgcgtaat caggtctcgg tagagaagcc ggactggtca
    47101 acggtaccgt ttatcatggc cgatcagggg ccagtacgcc gccgcattga actgtggttt
    47161 cgacgcaata aaatcagcaa cccaatgatt tacgccacgg ttggcgggca tgaagcgatg
    47221 gtatcgatgg tggcgctcgg ctgtggtgtg gcgctgttgc cggaagtggt gctggaaaac
    47281 agcccggagc ctgtacgcaa ccgggtaatg attttagatc gcagcgatga gaaaacgccg
    47341 tttgaactgg gcgtgtgtgc acaaaaaaag cggctgcatg agccgctgat tgaggcgttc
    47401 tggaggattt tgccgaacca caaatgacgt tgtcgcgcga gtaggcctga taagcgaagc
    47461 gctatcaggc atttttccct aacccgccaa aaagaacctg aacgccgggt tattggtttc
    47521 gtcgtggcaa tcgtagccca gctcatttag ccgggtttcg aaatccggtt catggtcgcc
    47581 aagctcgaac gccgccagta cacgcccgta gtcggtaccg tgactgcgat aatggaacag
    47641 agaaatattc cagtgcgtac ccagcgtgtt gaggaagcgc agcagcgcac ccggcgattc
    47701 cggaaattcg aagctgtaga gacgttcctg caacggatgc gatggacgcc ccccaaccat
    47761 atagcgcacg tgtaacttcg ccatttcgtc gtcggagaga tcaaccacgc tgtagccgcc
    47821 gtcgttgagc atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc
    47881 gacaaagatg caggcgtttt tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg
    47941 cccgccaagc agttggcaga atttgaggaa gctgcctttt tcttccggaa tggtcaccgc
    48001 caacaacgct tcacgctgtt cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg
    48061 gaagttcacg ttggcaccgg aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg
    48121 ggcgatatat tttttcattc ccgccagcgc cagcgcgcca gagggttccg ccaccgcgcg
    48181 cacatcttcg aataaatcct tcatcgccgc acagatcgca tcactatcga cggtgatgat
    48241 atcgtcgaga tactcctggc acaaacggaa ggtttcgtcg ccgatgcgtt ttaccgcaac
    48301 gccttcagca aacagcccca cgcgcggcag atcaaccgga tgacccgcat ccagcgctgc
    48361 tttcaggcag gcggagtctt ccgcttctac ggcgatcact ttgatttgcg gcatcagttg
    48421 tttgatcagc accgccacgc ccgcagccag accgccgccg ccgactggca caaatacgcg
    48481 gtcgagatgg gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc cggcaatcac
    48541 catcggatga tcgaacggcg gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc
    48601 tttggctttc gcttcatcaa agttcgcgcc gtggagcagc acttcgccgc cgaagccgcg
    48661 caccgcgtcg actttgatat cggcggtggc ggttggcatg acgatcagcg ccttcacgcc
    48721 taaccgtgcg gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag cagtgatcac
    48781 gccgtgcgct ttttgttctt ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt
    48841 aaagctgtgc actggctggc gatcttcgcg cttcaccaga atgacgttat caagacgcga
    48901 cgacagtttt tccatttttt gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg
    48961 cagcactgct cttaaatatt cggcaccttc cggagcaccg gacaggggtt gcgagtcagc
    49021 cattattaac cccccagttt cgatttatcg cgcaccgcgc ctttgtcggc gctggttgcc
    49081 aggctggcat aagcacgcag ggcaaaggag acttgacgtt cacgattttt cggcgtccag
    49141 gctttgtcac cccgagcttc ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc
    49201 tgtaactgaa tgccacggtt cggaatgtcg atggcgatca ggtcgccgtc ttcaatcagg
    49261 ccaatgctgc cgccgcttgc cgcttccggt gagacgtggc cgatagaaag gccagaggtg
    49321 ccgccagaga aacgaccgtc ggtgatcagc gcacaggctt tgccgaggcc cattgatttc
    49381 aggaagctgg ttgggtagag catttcctgc atgccagggc cgcctttcgg accttcatag
    49441 cggatcacca ccacatcgcc cgcgacaacc ttgccgccga gaatcgcttc taccgcgtcg
    49501 tcctggcttt cgtatacttt cgccgggccg gtgaatttga ggatgctgtc atcgacgccc
    49561 gcggttttaa cgatgcagcc gttttctgcg aaattaccgt agagcactgc caggccgccg
    49621 tctttgctgt acgcatgctc cagcgagcga atacagccat ttgcgcgatc gtcgtccagc
    49681 gtatcccaac ggcaatcctg cgagaatgcc tgtgtagtac gaatgcccgc cgggcctgca
    49741 cggaacatat tttttaccgc gtcatcctgg gtcagcataa cgtcgtattg ttccagcgtt
    49801 tgcggcaacg tcaggccaag tacgtttttc acatcacggt tcagtaaccc cgcacgatcc
    49861 agctcgccga gaataccgat aacaccacca gcacggtgaa catcttccat atggtatttt
    49921 tgggtgctcg gcgcaacttt gcataactgc gggactttac gcgaaagttt gtcgatatca
    49981 ctcatggtga agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag tacggtgtta
    50041 gtcgatccac ccatcgcgat atccagcgtc atggcatttt caaacgccgc tttgctagca
    50101 atattgcgag gcagtgcact ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca
    50161 atgcgtttac cagcattgag gaacagctgc ttacggtcgg cgtgagttgc cagcagcgag
    50221 ccgttgcccg gctgcgacag gcccagcgct tcggtcaggc agttcattga gttagcggta
    50281 aacatcccgg agcaggaacc gcaggttggg catgcggaac gttcaacctg atcgctctgg
    50341 gagtcagaaa ctttcgggtc tgccccctgg atcatcgcat caaccagatc gagcttgatg
    50401 atctgatcgg acagtttggt tttcccggcc tccatcgggc cgccggaaac aaagatcacc
    50461 ggaatattca ggcgcagaga ggccatcagc atccccgggg tgattttgtc acagttagag
    50521 atgcagacca tggcgtcggc gcagtgggcg ttgaccatat actcaacgga atcagcgatc
    50581 agttcgcgag atggcagtga ataaagcatc cccccatggc ccatggcaat cccatcatcc
    50641 accgcaatgg tgttgaactc tttggcaacg ccgccagccg cttcaatttg ttcggcgacc
    50701 agtttaccga gatcgcgcag atggacgtga cccggtacaa attgggtgaa cgagttcaca
    50761 accgcgataa tcggcttacc gaaatcggcg tcggtcattc cggtggcgcg ccacagcgca
    50821 cgagcacccg ccatattacg accatgagtg gtggtggcgg aacggtactt aggcatactt
    50881 tatttactcc cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat
    50941 ttattgatta acttgatcta accagcccca tttatcttcg gtttcgccgg tgaagaggcc
    51001 gaagaaggct tgctgaatac gtttggtaac cgggccacaa cggccttcgc caacctgaat
    51061 accgtctacg ctgcgcactg gcgtgatttc tgccgccgta ccggacataa acacttcatc
    51121 cgccaggtac agggattcgc gcgacagtac ctgctcacgc acttcaattc ccagctcttt
    51181 cgccagtttg atgatggcat cacgggtaat acccggcagc gcggaggagg tgaacggtgg
    51241 ggtgaacagc acaccatctt tcacttcaaa caggttttcg cctgcgcctt cagagatgta
    51301 accattcaca tccagcgcga taccttcctg ataaccgtgg cggcgtgctt cgctacccac
    51361 cagcagggaa gagagatagt taccaccggc tttcgccgcg gttgggatgg tgtttggtgc
    51421 tgcacggttc caggaggaaa ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag
    51481 atacgctccc cacgggaaag cggcgataat cacatcggtt gagtatcccg ctggcgggtt
    51541 aacgcccata ccaacatcac cgacgaagat cagcggacgg atataggcgc tggtgagatt
    51601 gtttttgcgg atcacgtcac gacaagcttc catcagctca tcaatgctct gcgaaaccgg
    51661 gaagcgatag attttggcgg agtcatgcag acgctgcata tgctcacgat ggcggaatac
    51721 aaccggtcct ttgtgcgagt cgtagcaacg gatgccttca aaaaccgagg tgccatagtg
    51781 cagcgcgtgc gacatcacat gcaccttcgc gtcttcccag cgaaccatct ccccattgaa
    51841 ccaaatgtaa tcagctttct tcgtggtcat ttttatattc cttttgcgct caggcgcgga
    51901 tttgttgtga tgtggttgtg ctctggcaga tggcaacgtg tgcgacgtcc accagtttat
    51961 ttaactgact aaacagtaag tcgaccgacc gtgggctggc aacggtcaat tcgatattta
    52021 tattttgtgc atcgctggcg gcggccatat tcattgagca gacgtggaaa ccacgatgac
    52081 gcaccacgcg taaaacacgt tctaaggttt ctggattgaa gcgagccgat acattgacct
    52141 gatgttgcat catgataatt tctccaacat ttctgaatta ctggcgccag gcggcaccag
    52201 cggccagacg ttctcaagtt cgtcgattga gacatgaagc aggtatggcc catcactgtt
    52261 cagcatggtg tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt ggccagggat
    52321 gccgaaggcg ctggctaaca tgaggaaatc ggggttatca gtaagggtgg tttcgctgta
    52381 tcgttcctga aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt ggttatcgag
    52441 taagacgatt ttcaacggta actgcttgcg ttttacggtg cccagctctt gcacattcat
    52501 catgaaagag ccgtcaccgg agatacagac aacggtatcg ttcggtcgcg cgacttgtgc
    52561 gccaaccgcc gccggtaaac caaaacccat ggtgcctaag ccgctggagg taatgaaatt
    52621 ttccgggcga gtgtgtgcga tgtgctgcgc ggcccacatc tgatgctgcc ccacatctgt
    52681 ggtcacaacg caatccgcag gtttacgatc cgacagttgt tttaacaaca acggcgcgta
    52741 gatagtgtca ccgggatggt cgtaacgcca ggcatgttca tcactcagct gcgcgcagta
    52801 ttgctgccag tcattgatat ttaatggctg ctgtaatgct ggtaacagcg catttaaatc
    52861 accctgtaat gccacatgtg cctgacgcag cttgttcatt tctgccgggt cgatatccat
    52921 atggataaca ctggcgtgtg gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa
    52981 acgtgcaccc acggcgatca gcaagtcgca ctcctgcacc gcgaagttcg ccgctttggt
    53041 gccgtgcatt cccagcattc ccagatagta cgaataatct gctgcaactg cgcccagccc
    53101 tttcagcgtg caggtggcag gcatttttgt ggtagagaga aattctcgta aagcaggaac
    53161 tgcctgcgcc atacccacgc caccaccaac gtacagcatc ggtttttgcg cttttgccag
    53221 catctggcgc gcttgctcga cttcggcatg ggggaaagtc acttcgtttt caacggtggt
    53281 gaaccacggt tccaggtcgc cgctggctaa ctggatatct tttgggatat cgaccagaac
    53341 cggaccagga cgacctgagc tggcaacgtc gaatgcttca gccatgatgc gcggcaactc
    53401 ttccagcgac tgcaccagga agctgtgctt agtacaggct aacgacaatc ccaggacatc
    53461 cacttcctga aatgcgtcag tgccgataaa cggtgcggac acttgaccgg tgatggcaac
    53521 aacagggata gaatctaaca gtgcgtccgc aagcccggtt atcaggttgg ttgctcccgg
    53581 accagacgtg gcgatacata cgccagtttt gccggtagca cgagcataac cgatagccgc
    53641 cattgccgcg ccctgttcat gtcggcacag caagtgctcc acgccgccgt catacaatgc
    53701 atcgtaaacc ggcataattg cgccacccgg ataaccgaaa acggtgttca caccctgtgc
    53761 ccgcaacgca tgtaccaccc actgtgcgcc attcatagtt agttccccgt cctgaatctt
    53821 gagaaacaga attttgtgct gttattcatt gtctgctcct cggttatgtt tttaaggtca
    53881 aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag
    53941 cctttcctcg tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca
    54001 cgaccaggct aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga
    54061 aggaatgcct aaagagttac catagatttt gcgaatagca caagatattt tttggccgat
    54121 ttttatgatg cctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga
    54181 gtgaaaaaaa ttcaatttca gtataaatgt tgttcccttc ctcgtagttc atcctttgtc
    54241 cgctgcattt ttgcgagcat cattccaccg ccagaacaac tcaacttcac ttgcacaaat
    54301 aaacaggaat cagccactaa aattcacgaa ttagcgggct acagcagcga tatcttatgc
    54361 acataatggt gactcaagga gggcttatgt cactgtcaat tgttcatacc cgcgcagccc
    54421 tgggagtaaa tgcgccccca atcactgttg aggtacatat cagtaaaggt ctacccggct
    54481 taacgatggt gggcttacca gaaacaacgg taaaagaagc ccgcgatcgc gtgcgcagcg
    54541 ccattatcaa tagcggatat gaatatccgg ccaaaaaaat caccatcaac ctagctccag
    54601 ccgatctgcc aaaagaaggg ggacgatatg atttacctat cgccattgcg ttgctggcgg
    54661 cctcagaaca gcttacagcc aataagttag atgaatatga attagtcgga gaactggcgc
    54721 ttacaggcgc tctgcgtggc gttcccggtg caatctccag tgcaactgaa gctattaagt
    54781 cgggcagaaa aattatcgtc gcgaaagata acgaagatga agtggggcta attaacggtg
    54841 aaggatgcct gatagccaat catctgcagg ctgtctgtgc gtttctggaa ggtaagcaca
    54901 ctctcgaacg cccgaaacca actgatgcag tatcccgggc gctacaacat gatctcagtg
    54961 atgttgtcgg tcaggagcaa ggaaagcgag gactggaaat taccgccgct ggcgggcaca
    55021 accttttact gatcggaccg ccgggaacag gtaaaacaat gctcgccagc cgtattaatg
    55081 gtcttttgcc agatttaagc aatgaagagg cactggagag cgctgcgata ttaagtctgg
    55141 taaatgctga atcagtacaa aaacaatggc ggcagcgccc gttccgctca cctcatcaca
    55201 gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc agggcctggt gaaatttcgt
    55261 tggcacataa cggcgtgctt tttcttgatg agctacctga atttgaacgg cgtacactgg
    55321 atgctttgcg agagccgatt gaatccgggc agatccatct ttcacgcaca cgagcaaaaa
    55381 taacctatcc agcccgtttc cagcttgttg cggcgatgaa tcccagccct accggacatt
    55441 atcagggaaa ccataaccgc tgcacgccag aacagacatt acgttatctc aaccggctct
    55501 cggggccctt tctcgaccgc ttcgatctct cactggagat cccattacca ccccccggca
    55561 ttttgagtaa aacggtagtg ccgggagaaa gcagcaccac cgttaaacaa cgtgtaatgg
    55621 ctgccagaga gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg
    55681 aaatacgcaa attctgcaag cttgagagcg aagatgcgca gtggctggaa gaaacgctga
    55741 tccatctggg gttatcgatt cgtgcctggc agcggttatt gaaagttgca cgaaccattg
    55801 ctgatattga tcagtctaac attatcacac gtcagcattt gcaggaggca gttagctatc
    55861 gtgcgattga ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt
    55921 cgcccttttt attaatcgtc agaatcggtg tagtcttcag caccttcaac ctgcggttta
    55981 ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac
    56041 acgcgttctg cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc
    56101 tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga
    56161 cgctcaagca gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga
    56221 taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct
    56281 cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta
    56341 aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa
    56401 ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg
    56461 ctctatctga cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt
    56521 gtgaaccttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta
    56581 ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgtaagga ggtggcgcat
    56641 acctcacgac ataacgagtt ttctatcggt gccagcgcct cattgtggga atgtatgctt
    56701 aatcagtggc tgggacgttt gtatcaaaat caggatgccc atacaggctt acagttcgaa
    56761 gcgcgaattg cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt
    56821 cttattacca ctgaagcgcc caaaatggac gaatttagta gtcagttgct ggggtatttc
    56881 actttagcgc tttataccag tgccccttcc aaactaaagg gagatcttaa ctatctgcga
    56941 ctggagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt
    57001 cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt
    57061 tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg gcctacacac cgttgtcgat
    57121 agcacaacac tttcgcggcc gctttatgcc atatggctgc aaaatagcga taaaaatacg
    57181 ctgattcgcg atctattgaa aattaacgtg ctggatgaag tgtattaatc tgaatggctg
    57241 gcaaggatgc cggtagaagg atttacttca gagagggtta tttcagataa aaaaaatcct
    57301 tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg
    57361 ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg cggaacggac
    57421 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    57481 ctccaccgaa ttcttttaca accaccggtt ttatgaccgg ctt
//
LOCUS       DAZGCQ010000016        57473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000016 DAZGCQ010000000
VERSION     DAZGCQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.016"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..1580)
                     /gene="wzxC"
                     /locus_tag="ACHK07_001089"
     CDS             complement(102..1580)
                     /gene="wzxC"
                     /locus_tag="ACHK07_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO8101453.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVVVFFLSDAI
                     GDVLNNPDLAPLIKTLSLAFVMIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTVGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(1582..2976)
                     /gene="wcaJ"
                     /locus_tag="ACHK07_001090"
     CDS             complement(1582..2976)
                     /gene="wcaJ"
                     /locus_tag="ACHK07_001090"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO8101454.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(3031..4401)
                     /gene="cpsG"
                     /locus_tag="ACHK07_001091"
                     /gene_synonym="manB"
     CDS             complement(3031..4401)
                     /gene="cpsG"
                     /locus_tag="ACHK07_001091"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO8101455.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTSLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(4594..6030)
                     /gene="cpsB"
                     /locus_tag="ACHK07_001092"
     CDS             complement(4594..6030)
                     /gene="cpsB"
                     /locus_tag="ACHK07_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO8101456.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGELTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(6033..7256)
                     /gene="wcaI"
                     /locus_tag="ACHK07_001093"
     CDS             complement(6033..7256)
                     /gene="wcaI"
                     /locus_tag="ACHK07_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO8101457.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQC
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAAERLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(7253..7732)
                     /gene="gmm"
                     /locus_tag="ACHK07_001094"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(7253..7732)
                     /gene="gmm"
                     /locus_tag="ACHK07_001094"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO8101458.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(7735..8700)
                     /gene="fcl"
                     /locus_tag="ACHK07_001095"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(7735..8700)
                     /gene="fcl"
                     /locus_tag="ACHK07_001095"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO8101459.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(8703..9824)
                     /gene="gmd"
                     /locus_tag="ACHK07_001096"
     CDS             complement(8703..9824)
                     /gene="gmd"
                     /locus_tag="ACHK07_001096"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO8101460.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(9851..10399)
                     /gene="wcaF"
                     /locus_tag="ACHK07_001097"
     CDS             complement(9851..10399)
                     /gene="wcaF"
                     /locus_tag="ACHK07_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO8101461.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(10415..11161)
                     /gene="wcaE"
                     /locus_tag="ACHK07_001098"
     CDS             complement(10415..11161)
                     /gene="wcaE"
                     /locus_tag="ACHK07_001098"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO8101462.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSS
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRRLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKS"
     gene            complement(11287..12507)
                     /gene="wcaD"
                     /locus_tag="ACHK07_001099"
     CDS             complement(11287..12507)
                     /gene="wcaD"
                     /locus_tag="ACHK07_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO8101463.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(12482..13699)
                     /gene="wcaC"
                     /locus_tag="ACHK07_001100"
     CDS             complement(12482..13699)
                     /gene="wcaC"
                     /locus_tag="ACHK07_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO8101464.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(13696..14184)
                     /gene="wcaB"
                     /locus_tag="ACHK07_001101"
     CDS             complement(13696..14184)
                     /gene="wcaB"
                     /locus_tag="ACHK07_001101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO8101465.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNSVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(14187..15026)
                     /gene="wcaA"
                     /locus_tag="ACHK07_001102"
     CDS             complement(14187..15026)
                     /gene="wcaA"
                     /locus_tag="ACHK07_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO8101466.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(15119..17281)
                     /gene="wzc"
                     /locus_tag="ACHK07_001103"
     CDS             complement(15119..17281)
                     /gene="wzc"
                     /locus_tag="ACHK07_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO8101467.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAYRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(17284..17727)
                     /gene="wzb"
                     /locus_tag="ACHK07_001104"
     CDS             complement(17284..17727)
                     /gene="wzb"
                     /locus_tag="ACHK07_001104"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO8101468.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(17733..18872)
                     /gene="wza"
                     /locus_tag="ACHK07_001105"
     CDS             complement(17733..18872)
                     /gene="wza"
                     /locus_tag="ACHK07_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO8101469.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            19181..19330
                     /locus_tag="ACHK07_001106"
     CDS             19181..19330
                     /locus_tag="ACHK07_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101470.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            19531..21114
                     /gene="yegH"
                     /locus_tag="ACHK07_001107"
     CDS             19531..21114
                     /gene="yegH"
                     /locus_tag="ACHK07_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8101471.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(21566..23419)
                     /gene="asmA"
                     /locus_tag="ACHK07_001108"
     CDS             complement(21566..23419)
                     /gene="asmA"
                     /locus_tag="ACHK07_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO8101472.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     TAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQILRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(23441..24022)
                     /gene="dcd"
                     /locus_tag="ACHK07_001109"
     CDS             complement(23441..24022)
                     /gene="dcd"
                     /locus_tag="ACHK07_001109"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO8101473.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(24114..24755)
                     /gene="udk"
                     /locus_tag="ACHK07_001110"
     CDS             complement(24114..24755)
                     /gene="udk"
                     /locus_tag="ACHK07_001110"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO8101474.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            25073..28390
                     /gene="dgcE"
                     /locus_tag="ACHK07_001111"
     CDS             25073..28390
                     /gene="dgcE"
                     /locus_tag="ACHK07_001111"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8101475.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(28499..29347)
                     /gene="alkA"
                     /locus_tag="ACHK07_001112"
     CDS             complement(28499..29347)
                     /gene="alkA"
                     /locus_tag="ACHK07_001112"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO8101476.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMSLKRAEALIHLANAALEGSLPMAIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            29481..30833
                     /gene="yegD"
                     /locus_tag="ACHK07_001113"
     CDS             29481..30833
                     /gene="yegD"
                     /locus_tag="ACHK07_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8101477.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQRVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALEEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(30846..32786)
                     /gene="yegI"
                     /locus_tag="ACHK07_001114"
     CDS             complement(30846..32786)
                     /gene="yegI"
                     /locus_tag="ACHK07_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO8101478.1"
                     /translation="MKPTLYTATGECVTTGRELGKGGEGAVYDINEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSAVSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRT
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMELLRQAAEKLAQAQADLSRC"
     gene            complement(32783..33544)
                     /gene="pphC"
                     /locus_tag="ACHK07_001115"
     CDS             complement(32783..33544)
                     /gene="pphC"
                     /locus_tag="ACHK07_001115"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO8101479.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(33541..34200)
                     /gene="yegL"
                     /locus_tag="ACHK07_001116"
     CDS             complement(33541..34200)
                     /gene="yegL"
                     /locus_tag="ACHK07_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8101480.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(34421..34480)
                     /gene="ibsA"
                     /locus_tag="ACHK07_001117"
     CDS             complement(34421..34480)
                     /gene="ibsA"
                     /locus_tag="ACHK07_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO8101481.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(34753..34809)
                     /locus_tag="ACHK07_001118"
     CDS             complement(34753..34809)
                     /locus_tag="ACHK07_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8101482.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(35081..35137)
                     /locus_tag="ACHK07_001119"
     CDS             complement(35081..35137)
                     /locus_tag="ACHK07_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8101483.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            35416..36663
                     /gene="mdtA"
                     /locus_tag="ACHK07_001120"
     CDS             35416..36663
                     /gene="mdtA"
                     /locus_tag="ACHK07_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO8101484.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNEPQSAAPGATKQAQ
                     HPPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            36663..39785
                     /gene="mdtB"
                     /locus_tag="ACHK07_001121"
     CDS             36663..39785
                     /gene="mdtB"
                     /locus_tag="ACHK07_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO8101485.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKIFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAHVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMLAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            39786..42863
                     /gene="mdtC"
                     /locus_tag="ACHK07_001122"
     CDS             39786..42863
                     /gene="mdtC"
                     /locus_tag="ACHK07_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO8101486.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            42864..44279
                     /gene="mdtD"
                     /locus_tag="ACHK07_001123"
     CDS             42864..44279
                     /gene="mdtD"
                     /locus_tag="ACHK07_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101487.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPIGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            44276..45679
                     /gene="baeS"
                     /locus_tag="ACHK07_001124"
     CDS             44276..45679
                     /gene="baeS"
                     /locus_tag="ACHK07_001124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO8101488.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            45676..46398
                     /gene="baeR"
                     /locus_tag="ACHK07_001125"
     CDS             45676..46398
                     /gene="baeR"
                     /locus_tag="ACHK07_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO8101489.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            46589..46921
                     /gene="yegP"
                     /locus_tag="ACHK07_001126"
     CDS             46589..46921
                     /gene="yegP"
                     /locus_tag="ACHK07_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO8101490.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            47119..47416
                     /locus_tag="ACHK07_001127"
                     /pseudo
     CDS             47119..47416
                     /locus_tag="ACHK07_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            47418..47714
                     /locus_tag="ACHK07_001128"
     CDS             47418..47714
                     /locus_tag="ACHK07_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8101491.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            47817..49178
                     /gene="yegQ"
                     /locus_tag="ACHK07_001129"
     CDS             47817..49178
                     /gene="yegQ"
                     /locus_tag="ACHK07_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO8101492.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(49508..49825)
                     /locus_tag="ACHK07_001130"
     CDS             complement(49508..49825)
                     /locus_tag="ACHK07_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101493.1"
                     /translation="MKKIVAISLFSIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CVE"
     gene            50230..51129
                     /gene="yegS"
                     /locus_tag="ACHK07_001131"
     CDS             50230..51129
                     /gene="yegS"
                     /locus_tag="ACHK07_001131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO8101494.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIVLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDNIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(51211..51984)
                     /locus_tag="ACHK07_001132"
     CDS             complement(51211..51984)
                     /locus_tag="ACHK07_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8101495.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(52090..53130)
                     /gene="gatD"
                     /locus_tag="ACHK07_001133"
     CDS             complement(52090..53130)
                     /gene="gatD"
                     /locus_tag="ACHK07_001133"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8101496.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIVHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(53178..54552)
                     /locus_tag="ACHK07_001134"
                     /pseudo
     CDS             complement(53178..54552)
                     /locus_tag="ACHK07_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(54556..54840)
                     /gene="gatB"
                     /locus_tag="ACHK07_001135"
     CDS             complement(54556..54840)
                     /gene="gatB"
                     /locus_tag="ACHK07_001135"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO8101497.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(54871..55323)
                     /gene="gatA"
                     /locus_tag="ACHK07_001136"
     CDS             complement(54871..55323)
                     /gene="gatA"
                     /locus_tag="ACHK07_001136"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO8101498.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(55333..56595)
                     /gene="gatZ"
                     /locus_tag="ACHK07_001137"
     CDS             complement(55333..56595)
                     /gene="gatZ"
                     /locus_tag="ACHK07_001137"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO8101499.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            complement(56624..57472)
                     /gene="gatY"
                     /locus_tag="ACHK07_001138"
     CDS             complement(56624..57472)
                     /gene="gatY"
                     /locus_tag="ACHK07_001138"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO8101500.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKKYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
BASE COUNT        13557 a        15460 c        14586 g        13870 t
ORIGIN      
        1 cgcgtcttat caggcctaca ggtcccgagc acagaaccgt aggatggata aggcgtttta
       61 cgctgcaccc ggcaaccgtt gtcggagcct aaaacaacca ttcaccccgc ccgtaaaagc
      121 attttcattt tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgc
      181 gacagcacaa tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc
      241 atccccagcg ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc
      301 ggcagagaga gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaaccg
      361 agcaccggtt taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg
      421 aagccaaccg tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata
      481 aacagaaatg ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc
      541 agcagcgaac caatcgggtt acccaccgaa cgcagcagac ccaccacaca cagcaattgc
      601 agcaccggaa taatgctgtt ccacttctca ccaaagacca gcggtacaaa gttattcgac
      661 accaccatca gccccagcag agccggaaag ttgataatcc ccactaccga cagcagcttg
      721 tagaagttaa cgcgcagctt ttcggtgtcg tcctgaattt tggcgaatgc cggaaacaac
      781 acgcgggtga tgattgggtt cagcttcatc ggtggcacaa cggccacgtt gtacgccagg
      841 ttgtatcccc ctgccacgcc cgcgccgaga atacgcgcca gcaccagcgt tgaaaggttg
      901 gtattgagat agttgatgat gctgtccgcc gtcagccagg caccaaagcg taagttcggt
      961 gccaccgacg ccagcgagaa atgcagaccg gggcgataaa ttttgcggcc aaagtagcca
     1021 aacagcagcg ttcttaccgc actattgacc agataaccga ggatcgcggt catcgccagc
     1081 ggccagaaat gggcgctaac caccgtaaaa gtgaagcccg ccagtactgc gctggtttcg
     1141 atcatgccga ttttgttgaa ctccagctcc ttctgcatca gtgcgcggaa ctgctgcccg
     1201 tgggggatca tcacaaacgc cagcgacaat gttttaatca acggtgccag gtccgggtta
     1261 ttcaacacgt caccaatggc atcactcaaa aaaaacacca ccacgcacac cacgatcccc
     1321 agcccgacgt tcagccagta caacgtggtg agttccaggt gactgatttc ttttcgctga
     1381 ataatcgagt tagcgatacc aaagtcagaa agcgtatctg ccagcgcgat aatcaccagc
     1441 gacacggtca gcaaaccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc
     1501 accagcccga ggccgataat gatcaccgtg gcaatcgccg accacttcgc gccgctgatg
     1561 gttttttcac gtaagctcat atcaatatgc cgctttatta acgaagccct tgaataccgt
     1621 caggaaaacg attttgatat cgaaccagac gctccattcg cgaatgtact caaggtcgaa
     1681 ttcgacgcgt ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg
     1741 cgcccagcca gtaatgcccg gtttcacctt atggcgcagc atgtagcctt caatgagctg
     1801 gcgatattgt tcgttatgcg ccactgcgtg cggacgtgga cccacaatcg acatcccccc
     1861 ggtcagcaca ttgataaact gcggcagttc atccagcgaa gtgcggcgca gaaagttccc
     1921 cactttagta acgcgcggat cgttctgcgt cgcctgagtc accactttgt cgttctccat
     1981 cactttcatg gaacggaact tccacacttt gatcggcttg ccatccatgc cgtagcgagt
     2041 ctggcggaaa ataaccggcc ccggtgaact cagtttcacc gccagcgcaa tacagcacag
     2101 caccggggaa atcagcagta aaatcagcgt tgccagcaca atgtcttccg cacgttttag
     2161 caggcggtta accccggaaa gcggcgtgtc gtacagcggc accacaggta cgccgttcat
     2221 ctcttcgata cgggaatgaa gaatattgaa ggtaaaaaca tcggggatca gcagcaccga
     2281 acaggtggta tccgccagtt gatggaccag ttttttcact cgcgcgccgt cgcacatctg
     2341 catcgcgata tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag
     2401 gttacccgcc cagtcgttag aaacgccgcc cggttttggg tcgtggtata cacccaccac
     2461 ttcaaaccct aaccacggct ggttacggaa gctctccatc agcatttgcc cggcggctaa
     2521 atcccccgcc accgcgacca tgcgcttgtt atagccatga ttacgcagcc agcccgcccc
     2581 aatgcgaata cacgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag
     2641 ccagattttc agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat
     2701 cacgcttaag gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg
     2761 ccatgagcga taaaaatcgg tgatgccacc cagcatctgg aacaccacca gcgtaatcag
     2821 cgccaccaac aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag
     2881 tccggcaaac atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt
     2941 ggttttcgct cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt
     3001 acccgcagtg gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg
     3061 cgttcgcgct tccatcagcg gcacatcacc gcgcgattcc acattcaacc gcaccaccgg
     3121 ttcggtgttg gaggagcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc
     3181 ggtgcgatcc accgccagcg cctcacggct aaaatgctgt tcgacgcggt taatcgcctc
     3241 aacggggtgc gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc
     3301 gcgcaccagt tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca
     3361 cgggatcatg ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc
     3421 gccaccgtag atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt
     3481 cgacatcacc ggcgtgccgc cagcagcagt caccacatca acagtgttcc aggagagacg
     3541 tggatcgtgg atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc
     3601 gacaatgtag tagccctcga taaactgccc tttttcgtca aacaggaaac agcggtcaaa
     3661 atcgccatca aaagcaatgc ccatatccgc gccgtgttta atgaccgcat tgcgggtgtc
     3721 gtcgcggcat tccggcagca gggggttagg aataccgttg gggaagttgc cgtccggtgt
     3781 gttgtgtact ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc
     3841 gtccaccacc ggacccgccg cgccgttccc ggagttgatc accagcttga gcgacgtgag
     3901 gtttttgacg ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg
     3961 ctgatagcga ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccaggcg
     4021 ctggacatca cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt
     4081 catgccgtta taatccatcg ggttatggct ggcggtaact tcgatgccgc catccacgcc
     4141 gagatggaac gtggcgaaat aaatctcttc ggtgccggac atgccaatat ccagcacatc
     4201 gacgcccgca tcctgtaaac ctttcgccag cgccagtttt aaggtttcgc tggtgaggcg
     4261 gacatcgccg cctaaaacaa tggttttcgg tttaagaaat tcgccatagg cgcgaccaat
     4321 acgccaggca atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt
     4381 aaagcaggtt aattttttca tatcgttacc ctttttcagg caatagttgg ccctgaccga
     4441 aacggccaat tttgtttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc
     4501 gacgctgccg cgtcttatca ggcctacaaa accgaaccgt aggttggata aggcgttcac
     4561 gccgcatccg acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa
     4621 gcgcaccaca tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg
     4681 aattttcccc gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tcgactcgtt
     4741 ttcaccaagc agtttgatat caccgtcaat agtgactttt gccgttcccg cgacaaccac
     4801 ccagtgttcc gcgcggtgat ggtgcatctg taccgacaag ccttcgcccg gtttcacggt
     4861 gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacgggcg
     4921 atagacttcg cgatgtaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac
     4981 ttttttcaca tcctgcaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac
     5041 cactaccaga tctttcaccc cgacggtggt tacgaggcca gattcggcat acacatagct
     5101 gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc
     5161 gctgatctcc cataatgaag accaggaacc gacatcgctc cagcccgcat ccatcggcac
     5221 cacaacggca tctgccgtac gttccatgac cgcgtaatcc accgactctt ccgggcaggc
     5281 cagaaacgct tcttcatcca cgcgaataaa atcgagatcc ggatcgacgg cactcattgc
     5341 tttttcacag gcatcgagaa tatccgggcg atattttttc agttcttcga gatagcgtcc
     5401 ggcgcggaac aggaacatgc cgctgttcca gtaatattcg ccacttgcca cataggcctg
     5461 ggcggtttcc agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg
     5521 ctcgcccgca gatacttcac cgcgacgaat atagccatat ccggtttcag gtagatccgg
     5581 cacaatgccg aaggtcacca gtttgcccgc ttcggcatat ggcatggcat tacgcacggc
     5641 ggcacggaac gcgtcttcat cggcaatcac atgatccgcc gccagcacca gcattaacgg
     5701 gtcgctctcc ggactatgac gtgttgccgc cagcgccgcc agcgcaatgg ctggtgcagt
     5761 gttacgccct gctggttcga gaataatgtt ctcggtgagt ttgttcagtt ggcgcagctg
     5821 ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac
     5881 gccgttcagg cggcagatgg tggtttgcag catggtgagt tcgcctttca ggcataaaaa
     5941 ctgcttggga taaagtacgc gggaaagcgg ccataagcgg ctaccggagc cacctgccat
     6001 cacaactgga tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga
     6061 cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttgtgt
     6121 ttgggcagca ggagcgcctg acgtatcccc gccaccagcg cctcgaccga ttccggttct
     6181 acgcaaaccg caatgcccgg aaaggtttcg caaagctgtc ccagttctgt gtgggcctct
     6241 gcggtaatca ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg
     6301 gcatctgccg cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgcg
     6361 ggtaaagcgt catacgattg cagcgggaag aattgcatgt tgtgcagtcc acgctgctgc
     6421 gccatttttt ccagccgcgc tttgccgccg ccctgcccga caatggcaaa aatcagcggt
     6481 tcatcacgca ggcgttcggc agcttcaata acgttttcca gcccctgctt ttcaccaata
     6541 ttgccggagt aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca
     6601 tcaacatcgg cgtctgcaac atgctgaaaa cgggcgattt ccgaccagtt ggggaagaaa
     6661 atgacgttat ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc
     6721 gtggagacgt tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc
     6781 actttgccgc ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa
     6841 tcctgaatat gcagcacggt acgcgcacca gagagtttcg ccagcaggcg cattcccggc
     6901 gtgcaaaaca gcgttggcac tacgccgata atgcgatccg gcttccagcg acattgcgcc
     6961 atcaacggga aaaaactgct gacggcaaaa ctgccgagat gtaacaggcg tttcagggtg
     7021 ctcggctgtt ttggcacgta cagcgggcag cgccacaccg tagcggcccc ctcttctcgt
     7081 ttgtagcgcc aggcggaata gttctcgccc acctgccact gcgggtagta aggcggtgcg
     7141 gtaatgaccc gcacctcatg accttgtgcc gccagccatt ccaccatctc gccggtgtat
     7201 ttgccgatgc cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc
     7261 cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg gctgttagcg tgaacattgt
     7321 cactggcgag cagcgcgtcc ggcgtcaacc agcggtaatc gtcatgctgc tcatccggca
     7381 gtaacagttc ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga
     7441 aatccgtgcc agagaagtta tcgtcataaa agtgctgcca gacaccgtaa aactggcctg
     7501 ctgttatcgg cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca
     7561 gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt
     7621 tggttctttt gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga
     7681 caagcggagt ggagcgcact accgtggcaa agtcttcctg acgtaaaaac atcattaccc
     7741 ccgaaagcgg tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag
     7801 tgagatttcg tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg
     7861 cgtaccatcc ggtttactgg catcaaaaac cacccgacct ttgtaaccca ccactttggc
     7921 gatggtttgc gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga
     7981 cagcatcggc tgggtgttct ccagccagac ttcgtgcgcc agctccatga catgaatgct
     8041 cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccacac
     8101 caccacgtcc ggtgcattct gtgccgtcgc ctcgtggaag cgacgcagca atgcagggat
     8161 cacatgcgaa ttactcgggt ggaagttgtc atgcgggcca tacaggttgg tcggcatgac
     8221 cgagcggtaa tcgcgtccat actggcggtt gtaagattcg cacagtttga tcccggcgat
     8281 tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg ccctgcaata actcgctttc
     8341 tgccatcggc tgttttgcca gtttcgggta gatgcaggac gatccgagaa acagcagttt
     8401 gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc tcaatcatca tgttctgata
     8461 gatgaaatcc gccggatacg tgttgttggc aacaatgccg cccactttcg cggccgccag
     8521 atagacctgg tcaatacgtt cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag
     8581 gttcagctcg tcgcgggtgc gtaataccag ttccacatca ccgcgctgtt cgagctgccg
     8641 cctgatggcg gaaccgacca tcccgcgatg gccagcaata aaaatgcgtt gtctactcat
     8701 acttatgact ccagcgcgat cgccacgtcg tagccgtgag atttcagcag agagtgtttt
     8761 ttcgccgctt cgaggtcatt agccaccatt tcagacacca tctctctgag ggtgatttcc
     8821 ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttca
     8881 gccggacgga agtaacgcgg atcaacggcg ataatcacat cacccggttt aacgcccggc
     8941 gcgtcatgtc cggtgacgga aaccacaatg cccttctctt caacgcccgt gccttcaaag
     9001 cgcagtttga tgcccagctg tgccgccgcc atttccacga actgacgtac ggagtactga
     9061 acgccggtag cgataacgaa atcttccggt tgttcctgtt gcagcatcat ccactgcatt
     9121 tttacgtagt ctttggcatg gccccagtca cgcagggaat ccatattgcc gaggtacagg
     9181 cacgattcca gcccctgggc gatgttggcg attgcgcggg tgattttgcg ggtaacgaag
     9241 gtttcgccgc ggcgcgggga ttcatggttg aagagaatac cgttgcaggc gtacatgccg
     9301 taggattcgc ggtagttaac ggtgatccag taggcgtaca gtttggcgac cgcatacgga
     9361 gatcgcgggt agaacggcgt ggtttctttc tgcggaattt cctgcaccag accatacagt
     9421 tcagaggtgg aagcctgata gaaacgggtt ttcttttcca gaccgaggaa gcggatcgcc
     9481 tcgagcaggc gcagcgtacc catcgcgtcg acgtcagcgg tatattccgg tgactcaaaa
     9541 gagaccgcaa cgtggctcat tgcccccagg ttgtacactt catccggctg tacttcacgc
     9601 aaaatgcgcg tcaggttgga ggtatcactc aggtcgccat aatgcagatg gaatttcggg
     9661 ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg
     9721 cgacgcttaa taccatgcac ctcgtaacct ttttccagca gaaactctgc caggtaagaa
     9781 ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg acatgtctta ttcctctgta
     9841 ttttttgaat ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc
     9901 aaaccacatt tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt
     9961 cgccaatcgt gacgccaggg gcaacaaaaa catctgtcgc cagccagcat ttctcgccaa
    10021 tcacaatagg cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc
    10081 ataaataact tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat
    10141 ataaattgac gtcatcgccg acccacgcgt aatcacctaa ggttaatttc cacggatagg
    10201 taatttttac tgacggacga ataactacgt tttttcctat ttttgcgccg aataaacgta
    10261 ataaaaaagc ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta
    10321 ctgcccacca taattgcact tttatagcgt tgccgccccg gaaccctttc ggcaccgaga
    10381 atccacttaa atcttgcatc gttttccctt atattcagct tttgttatat aaggctttag
    10441 tcttacctgt cgtacgctgg cgtaaatgcc aggataattc agcccagaag ccaggcacat
    10501 gtaatatttg acgttggact tttttggcgt cagcacacaa ttccatatta ttggtggtag
    10561 ataccccacc catagaaaat tcagacacca gtccattgag ttttttaaat gcataaccgg
    10621 ctttatacat tttggctgcc agcgcgtaat cagaagaaac tttatattcc aggtcataac
    10681 gccatttttt caagccggat accgggaaaa atatcgcctg atgactggcg ggcagactgt
    10741 gataaatata ccaacccggt ttggcgctac gtttaatttt atgcccgtcg ccaaaatcca
    10801 gtagcgcatc gccggtgatc atcacgttat ctttttgcac ttttaaccta cggacaaaat
    10861 ttgcggcatc ctgatgaaaa atatcacctg agttgagaaa caacgcgaac ttaccttgcg
    10921 ccatcgcaat gcctttgttc atggcgtcgt agataccgtt atctggctcg ctgacaaagc
    10981 gtaggttata gataccattg agattttcca ggtactcacg ggtgccgtca ctggaaccgc
    11041 catcgacaac aatccattcg aagctgatat cgtccgcctg cgccagatgc gccagcgagg
    11101 cgtgtgtttt gactatccct tcgaggttac gaaacgcgac agtgattatg ctaagcaaca
    11161 tgttttacct cgtaataccc gtcatacttc aagcggcatg tgcgttggtt ttcctcgctc
    11221 accccagtca cgtactcgtg tacgcttctg gggattcgct gcgtcaccaa cttcctgcaa
    11281 ctcggattat ttagggtgta tatttaacgc ttttcgcaaa ataaacggac agacgattaa
    11341 aaatgcatat tccgggctaa atatcgaacc ggtaaaaaac agcgataccg gagtaaaaag
    11401 atataattgc acgcgaaaat tgcggttatc accaaaagcg ttgatcatca tttttatcac
    11461 tttccccatg taccacagcg ataaaaacac cgcgaaccag gaaaaataaa taatcagcag
    11521 atacaaacca ttgtctatgg tttttccgac atccgcaccg ttaaatattc cgaatgatgc
    11581 gacatattca taaagtgagc caaatctgac tacaccgtca atatgggtca aagaataacc
    11641 gaccatcacc agcggaccga caatacgata atacgatgac gatccttccg tacctaaatc
    11701 gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaaccagga atacagccag
    11761 agaaatcaat gctaacggta actttttctt aatcgcctct ttattcagat attgaaatgc
    11821 ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc cggataatat
    11881 tatccctgcg agaatcatag catcggtttt aggcgttttg ataccaaact gtttgatgct
    11941 gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa gtgctgtcgt
    12001 gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa tcagatattt
    12061 cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca tctccaccgc
    12121 cgccagcgcc accaccagcc cgaccaccag ataaaagaaa cgtaatatct tgcgatggtt
    12181 gtgtggcgaa atcgttttaa agcgaatact ccacaccatg ccaataatga tcacaatata
    12241 gacaaacagc atggttgaag tgacgtattt actggcatcc aacgactgac caaacaggta
    12301 gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt taacgctgat
    12361 gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga tggggaaact
    12421 ttcgccaagc tgagcgattt tgacgttaac cagcaggtag atcagcggca gcagcaggta
    12481 gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg
    12541 cgcggctgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc gcgatttccg
    12601 gtttgcttaa ctggaccagt tgcagcacct cttcttcgct gacggtttta ccgccggatt
    12661 tttgcaacac ttcacgcgcc gcgtcgctat gggtggcaat caccggcacg ccaattgata
    12721 acgcctcaca caaaatcagc gggtagttat cgacgcgaga actgaatacc agcgcatcca
    12781 tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga ttaaccacgt
    12841 tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg tcgcccagcg
    12901 ccatcatctc gcgtaccagt tgctggttag ttttgccgtc gtaacgcagg tcatgcgcta
    12961 ccaccgcgat tttcggctta ccctgagttt cgcgcaccgg aggcaaatcc gccagaatcg
    13021 cctcggttgc catatcaata ccgttattga taatccggca acgccctgga ccgtacaggc
    13081 tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc agcgccagca
    13141 tctcacggaa taactggcgt ttgcccgcca ccagctggtg cgcccgatca atcttcaccg
    13201 gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt
    13261 cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag accagagtga
    13321 cgtccggttt gtggtttttc accttttcgc aaaagcgcac cacgctctta agatttagcc
    13381 agtagctgtg cagcacatga aaatgaagga ccaccggacc cggtgtgcga gtaatggtgc
    13441 gatataactc attgaaattg ccaaacagat cgcgattaaa cagacgaaac agggcaatat
    13501 tcgccattgc ggtcatccgc ggcgtatgtt tgattacctg cggatagttc tgatggctga
    13561 cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca
    13621 gcgcgcgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc agtcgcacat
    13681 taaattgcag aatattcatt taattacctt cactcgcgct ttttctccca ccaccagcgc
    13741 gttgtccggg acagagtcga gcaccacgct gcccgcgccc acggtgacgt tgttaccaag
    13801 cgtgatatca ccaagaataa tgacgttggc accgagttcg acgctgttgc caatgtgtgg
    13861 acatgccatg ttatcggcac cacgattgcc gatagtgacg ccgtggcgaa tggtgaaatc
    13921 atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga tagtaaagcg
    13981 gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg
    14041 atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt tgcgccacac
    14101 cgaacaaaaa tgagcgacgc gataggcaag aaccatgcag cacgggcgta aactccagct
    14161 gttggcgcgt agatcttcca gcatatttag cgcccccgaa taccatcagc cagacgtttg
    14221 ccgttacgca ccgacagcag cgtcagcaaa gtgcgccagg tcatacgttt attgcggatc
    14281 tggtagagag taaacagctg atatttttta ctggcacggt cgaatttgtc tttgtgcttg
    14341 cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcaccg
    14401 tgattgatat gcaggatctg cgtcgcttct tctactttcc acggttcgcc atactccacc
    14461 accatccgca ggaagatgtc gtaatcctgt gccgctttca gttcggtatc gaacaaacac
    14521 tctttgaaac gccacgccca ggtaaagacc tgattgccaa tgatattgcg tttatagaac
    14581 aggcggcgtg agtacggcga tttgggatac agtggtaagc ttgccggttg ggaataaact
    14641 tcgccctggc aaacgtaatc gttagcgtac aaaaaggcgt gagtgaccag ttgctgttta
    14701 tgggcgagga agacgctcag acggttgggt gtccattcgt catcgtcatc gatcccggta
    14761 atgtattccc cttgcgccag cataatagcc tggttacgta ccgcgcacgc cccgctgtta
    14821 atgtcgttgt gaatgtaggt aatacgcgga tcgttgaggg cggtgacgta ctgttgcagt
    14881 tgttcccaag aggtggagca atcatccacg atgatcatct cccagttgct gtagtcctgg
    14941 cgcaagaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt cggcatatag
    15001 attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc tggtttgctt
    15061 gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc aggcgtcgtt
    15121 atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc
    15181 ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc tcaaagcggc
    15241 tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg
    15301 ttccaacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa
    15361 tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca
    15421 tcaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa
    15481 tagaggtcgg tttagctgcg gtagtaatat cgccctgacc aatcagaatt tccgacaggc
    15541 cattgacgtt attggtgccc aacagctcgt gggtgtagcc tttgcgcata tcgcagtcga
    15601 tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg
    15661 ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc gcctgcatca
    15721 tcgcgaagtg cagactggta cgcagactgc ggatggcttc aatcgccaga tcggttggat
    15781 tccccaccgc cagtagctgg ctctgtttat agcgtttaac tcctttgatg gttttgacgc
    15841 tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg ataccgtgtt
    15901 cttccagcac ctgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca
    15961 cgatagagag catcaggccg aggataatcg ccccaaggat aatcagccct ttcttcggtt
    16021 ttagcacgcc aggctgggtg attgccgggt caacaatgcg cacatcaccg acggtgctgg
    16081 cctcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag
    16141 actcgacatc gcgggtcaga cggacaatct cctgctgggt tttcggcatc gccgttacgc
    16201 gaccgttaag tttggctttt tcgtcttcca gcgcctgacg tttctccagc agcgtgcggt
    16261 acgcggggtg aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt
    16321 tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttcc agcggcagat
    16381 caacagaatc tttatcctga cggtaggcat tcagtttgtt ttcggcaaca tcaaggcggc
    16441 tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt
    16501 tgcgctcaat attttgttcc tgatagttac gggcgatgct gttaagaatg tcgcggatct
    16561 gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg
    16621 ttaccgtcag gctgttttgc agttggttga tcatccccag cgtggagtat ttggtgacgg
    16681 taaactcact gcccgggctg gcgtgaatag cttcaaccat cagcgtgacg ccttcttttt
    16741 tcagcatctg gcccgcttgt ccacgggcgc taaagccgcc atcactgctc agggtgtagt
    16801 ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc ggacggttaa
    16861 aggtagtcac tttcaccgtc tcgttctgac gtcccatcag gcgatcccag cccgcaccga
    16921 aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac
    16981 caagcaccag gcgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg
    17041 ctaacgccga tccgatatcc tgcactaacg aattgccgct gctttgctcg atttgtacca
    17101 gtgcgtcggc actataaatc ggcgtggcga agaaggtgta aaccacggca caaagggcga
    17161 atacagcggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac
    17221 caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca
    17281 ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa
    17341 tgtgtacacc gctgcaaacg tttcccggct tttgcgatac ggatcgggga tttcacattc
    17401 gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca tctcgcataa
    17461 gcgttcgata tggcgctttt ccatggtcaa aatcaggtca tagttgcgac acaggcggcg
    17521 gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg cgacgctgat
    17581 agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact ccacttttag
    17641 ctccgggtga taacgttgca gtaagcgttc cgccgtcggg gaacggcaaa tattgccgac
    17701 acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta
    17761 tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag
    17821 cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc
    17881 gccatcacca gcgacgtggc atcggacata tccagctggt agatattggc aatcttgccg
    17941 ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg
    18001 gtcatgtcaa tgccttcagc attgcccaga gcttcagtca gggtcatgcc gctaaagtcc
    18061 attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta
    18121 cgcggcacgt agagaatatc gccggggtaa agcaggcgat tctggttgag gtcgccgttt
    18181 tgcatcagcg cctgcaaaga aatgcgctct tcacgaccat tgtgtgttag caccacgttg
    18241 cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt
    18301 ggtacgttgg tgatcgcctg ttgaccagat ttattcacct gacctgatat ataggccttt
    18361 tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag
    18421 cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg
    18481 atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg
    18541 cggtactgac cggctggcgt ggtgagttcc gggtgatccc agacggtgac attcagaacg
    18601 tcgcccggcc cgacgcgata ctggtaattc gcgatctcac tttccagcgt catattgggg
    18661 cgcgctacgt tgggtcgtgg acgtaattga tcaatcaggc gcggggtcaa cggataaaca
    18721 ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc
    18781 gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat
    18841 aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat
    18901 tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat
    18961 aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa
    19021 taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt tttagctacc
    19081 aatacactga tttagcttaa tttttaaaac cctctcaaca tacagttgtt gatgagaaag
    19141 ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct
    19201 ctccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg
    19261 gcaattggct atttttaaga attatattaa gtgtcattca atatggtttt taggagtttc
    19321 tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa ataatgtttt
    19381 attattacca cttttaattc agggataatg tgaggttatt accctcaaat aatatgagat
    19441 aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt
    19501 gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg
    19561 gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt
    19621 attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg
    19681 ctgttgctgg cgatgttaat gcgcctgtta ctgctggcgt caatctcctg gctggtcacc
    19741 ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg
    19801 ctgtttggtg gtttcttctt gttgttcaaa gccacgatgg agctgaacga acggctggaa
    19861 gggaaagaca gcaataaccc cacgcaacgc aaaggtgcga agttctgggg ggtggtgaca
    19921 caaattgtgg tactggacgc catcttctca ctcgactcgg tgattacagc cgtcgggatg
    19981 gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg
    20041 gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta
    20101 agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg
    20161 aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcgct caatcagttg
    20221 gctatcttta accggcgacg ttttctttcc gctaaccaga cgctgcgcca gcggactacc
    20281 gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccgaaacc
    20341 gcgtctatgc tggtggatca tggtaaccag cagatcttta atccgcagga acggcggatg
    20401 attgagcggg tacttaatct taaccagcgt accgtcagca gcattatgac gtcgcgccac
    20461 gatattgagc atatcgatct caacgcgccg gaagacgaga tccgccagtt gctggagcga
    20521 aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt
    20581 gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa cctgcgggtg
    20641 ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttacctgc tctggagcag
    20701 ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggttc agtggaaggg
    20761 attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa
    20821 gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt
    20881 catatgccgc tggaagatct ggtgcaatat gtgccgctac cgctggatga aaaacgtgaa
    20941 tatcacacca ttgccgggct gttgatggaa tatttacagc gtattccaaa acctggcgaa
    21001 gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag
    21061 aaggtgcaga ttattccgct acgtaaggat ggcgagatgg agtacgaggt gtgatggtat
    21121 cggtcatttt acccgatgct gcgtaacgcc ttataacttc agcaatgaag cgttgtctgt
    21181 ggtacatgga cattacagcg ggggtgtggg tccggctgta aatcgccgaa tcgtttcctg
    21241 taggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc
    21301 gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc ggcgatttac
    21361 cgccgggagc ccgggttgcc aggatggtgg cgattgagcc accctggcac gttcaccggc
    21421 ttgagcgttc catagtagtg aggaacatca agtgaacgga acagccttcg atcttacata
    21481 ttcagaatat aatggtgccg ccgcgaacgc ctgatgcgac gctttacgcg tcttatcagg
    21541 cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc acatctttgc
    21601 cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc gcttcgtcct
    21661 gtagatgttt gcgcagtatc tgatccactt gcagactgta attgagttgc tgccagttgc
    21721 cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg ctttccccgt
    21781 tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct gccagattca
    21841 acatgccttc ccctgtaagc gccagcacag gcgattgacc ttgcatgtcg tttaacgtca
    21901 cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagacgcgtc acgttatcga
    21961 agttttcagc ggccttcaca tcaccaccat tacgttctac cgcttgctga atcatctgct
    22021 ggaagttcat cccttccatg cgcgtatcgg ccatttcgac atgcgccgtc ccctgccagt
    22081 tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc agtgacatct
    22141 ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtgccaatc tcaacgtttt
    22201 ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc gtgcccggca
    22261 gtgaaacctg tccaccgtta agtttgccct gcagttgagt aatttccagc aaacccgact
    22321 tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc acgttactgg
    22381 cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg ataggccggt tcatcaatac
    22441 gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc gcggcaccgt
    22501 tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt tgcgggaatt
    22561 gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgc ccgctcagcg
    22621 tactatcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt gactcctgcc
    22681 actgggcctg gaaactcccc tgcccctgaa taccttgttt tggtaaatct gcaccctgca
    22741 actgccagtt aatttgttca atagccgccg ttaaatcatg cggataatca gaagcatcta
    22801 ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc ccggaaaact
    22861 caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcgaatattg cggattgtca
    22921 cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta agactggata
    22981 tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg cgcggtgcaa
    23041 ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc actgcccctt
    23101 ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc gccacgtcca
    23161 ggcgcatgtt gtcggcgcga accagtggct ggctcgcgcc ctgggcggtg agagacattc
    23221 gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg tcgagctgca
    23281 attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa tcattcggat
    23341 tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaggagt atcatcagcg
    23401 tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta tcgattcggc
    23461 tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg taaggtcgcg
    23521 ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc ggacgcagcg
    23581 ccagcggcag cttaccggag ttgtagaact ccagcacaat gcagccagac cagcccggat
    23641 cgatgcggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag gaacgcccgt
    23701 ccagccagcc caccagatcg gctggcagcg tcaccgactc cagcgtcacc gccagcgcca
    23761 gctctcctgg gtgaagatag aacgcctcgc tttcatcgag aacgatctca tcgctcatca
    23821 cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata aacgctgccg
    23881 tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc gcgccgttaa
    23941 tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc caggcttcaa
    24001 tatctcggtc acacagacgc atggcatttt ctcctttcgc gcatcactcc cttaacgcca
    24061 attacgtcaa gggcatacca ggttatcact gaacggtaca caatttatca agcttattca
    24121 aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt cccgccgcgc
    24181 ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa catcgggcgc
    24241 acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc gttaacgtca
    24301 cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat ggagaagtta
    24361 agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat gatgaccttc
    24421 ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct gtaaaccggc
    24481 aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcagcag gctgtgatcc
    24541 atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag atggctttga
    24601 tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac ttgctcacgt
    24661 aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc gccagcgata
    24721 ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg attaacctgg
    24781 atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt catccgcctg
    24841 ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag aattcccggt
    24901 ggcaaattac gttgatcagt tttatacaaa gtaaaaaatg ttatacgcag ttgcgcaaat
    24961 tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac ttttgtacta
    25021 gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg taatgagcaa
    25081 acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg tcagtttagg
    25141 tttagtctcg tttatcttta cccttttctc gcttgagctt tcgcagtttg gcacccaact
    25201 cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc atgccgggcg
    25261 catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat ccatcctgct
    25321 tttttccacc agctcgctga acatgacctg gacgaccatc aatattgttg aagccgtggt
    25381 cggggcagtg ctgctacgta aattgctgcc gtggtataac cccttgcaaa atctggctga
    25441 ctggctgcgt ctggcactcg gcagcgccat tgttccgcct ctgttggggg gtgttttggt
    25501 tgtcctgctg acgcccggag acgatcctct cagggcattt ttgatatggg tactgtcaga
    25561 atccatcggc gctctggcac tggtgccgct gggattgtta tttaaaccac actatctgct
    25621 gcgccatcgc aacccaaggt tgctttttga gtcgctgctc acgttagcca tcacactgac
    25681 gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt actttcatta ttgtgctgtt
    25741 gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt ttgatcttcc ttaccacggt
    25801 gatgatggtg tcgctgatga tggccgcgga tccctccctg cttgctacgc cgcgtacgta
    25861 cctgatgagc catatgccgt ggctaccgtt tttgctgatc ctgctgcccg ccaacatcat
    25921 gacgatggtg atgtatgcct ttcgtgcgga acgcaaacac atttccgaaa gcgaaacccg
    25981 ctttcggaac gcgatggaat attccgctat cggtatggcg ttagtgggca ccgagggaca
    26041 atggctgcaa tccaacaaag cactctgcca gtttctcggt tacagtcagg aagagctgcg
    26101 cggactcacc tttcagcaac tgacctggcc ggaggatctc aataaagatc tccaacaggt
    26161 tgaaaagctg ataagcggtg aaataaacac ctattcaatg gaaaaacgtt actacaaccg
    26221 caatggcgat gttgtctggg cgttgcttgc cgtctctctg gtgcgccaca cggatggcac
    26281 gccgctctat tttatcgctc agattgaaga cattaacgag ctaaaacgca ccgaacaggt
    26341 gaatcagcaa ctgatggagc gcatcacgct ggctaacgaa gcgggcggga ttggcatctg
    26401 ggagtgggag ctgaagccga atatttttag ctgggataag cggatgttcg aactgtatga
    26461 aattcctccg catatcaaac cgaactggca ggtgtggtac gagtgcgtgc tgccggaaga
    26521 tcgtcaacac gccgaaaaag tgattcgtga ttcgttgcaa tcacgctcgc cctttaagct
    26581 ggaatttcgc attgccgtga aagatggcat ccgccatatc cgcgccctcg ccaaccgggt
    26641 actgaataaa gaaggcgaag tcgaacgcct gctcggcatt aatatggata tgaccgaggt
    26701 taaacagctt aacgaggcat tgtttcagga aaaagagcgc ctgcacatta cgctggactc
    26761 cattggcgaa gccgtagtct gtattgatat ggcgatgaaa attaccttta tgaatccggt
    26821 ggcggagaag atgagcggct ggacgcagga agaagcatta ggtgttccgc tcctgacggt
    26881 gttgcatatt acttttggcg acaacgggcc attaatggag aatatttaca gtgccgacac
    26941 ctcacgttcc gcgatcgaac aagatgtggt gttgcactgt cggagcggcg gcagctacga
    27001 cgtgcattac agtattacgc cgttaagtac tctggacggc agcaatattg gttcggttct
    27061 ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc cagctgagct acagcgcctc
    27121 ccatgatgca ctgacgcatc tcgccaaccg cgccagtttt gagaaacaac tgcgtatcct
    27181 gctgcaaacg gtaaacagta cgcatcagcg acatgcactg gtgtttatcg atcttgatcg
    27241 ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc gacgctttgc tgcgcgaact
    27301 ggcgtcgttg atgctgagta tgctgcgctc cagtgacgtg ctggcgcggc tcggcggtga
    27361 tgaatttggt ctgctgctgc cagactgcaa tgttgaaagc gctcgtttta tcgctacacg
    27421 tattatcagt gctgtgaatg actatcactt tatctgggaa ggccgggtac atcgggtagg
    27481 tgccagcgcc gggattacct tgatcgatga caacaatcat caggcggctg aagtgatgtc
    27541 gcaggctgat atcgcctgtt atgcctccaa aaatggtggc cggggccggg tgacggttta
    27601 cgaaccgcag caagctgccg cacatagcga acgggcggcg atgtcgcttg atgaacagtg
    27661 gcggatgatt aaagagaatc agttgatgat gatcgcccac ggtgtcgctt cgccacggat
    27721 cccggaagcg cgtaatttgt ggctgatttc acttaagctc tggagttgcg aaggcgagat
    27781 tattgatgaa caaacatttc gtcgtagctt cagcgatccg gcgcttagcc atgctcttga
    27841 ccgccgggta ttccacgaat ttttccagca ggccgcaaaa gcggttgcca gtaaaggcat
    27901 aagcatcgct ctcccccttt ccgttgccgg tttgagtagc gccacgctgg tgaatgatct
    27961 gcttgagcag ctggaaaata gccctctacc accacggtta ttacatctga ttattccggc
    28021 tgaagcgatt ttagatcacg cagaaagcgt gcaaaaactg cggctggcgg gatgtcggat
    28081 agtgctcagc caggtgggcc gcgatctgca aatcttcaac tcgctgaaag cgaatatggc
    28141 agattacctg ctacttgatg gtgagttatg cgccaacgtg cagggtaatt tgatggatga
    28201 gatgctgatt acgattattc aggggcacgc tcagcgactc gggatgaaaa ccatcgccgg
    28261 gccagtcgtt ttacccttag tgatggatac gctttctggc atcggcgtcg atctgattta
    28321 tggtgatgtg attgccgatg cccaaccgct ggatttgctg gtgaatagta gttatttcgc
    28381 gattaactga ggcgtgcttc cccatcgcct gatgcgacgc taacgcgtct tatcatgcct
    28441 acaaatcgct cattccccag gccggataag gtgctcgcac cgcatccggc gaccaacgtc
    28501 atgcttcgtc tggttgccag ccttccgtat accagatatg caacagcgca taagaacgcc
    28561 agggcttcca gcgttcggca taacggcgga tttgtgccgg tgtcattccc ggaaatcgct
    28621 gtttaatcag ataatcatcc ggcaggaaaa catctttcgc ctgccagcca cgcaaagcga
    28681 aataattcgc cgtccagcgc ccgatacccg gaaaagtttg cagcgttttc atcgcctgct
    28741 ccacatcgcc cggaattgcc attggtaagc tgccctccag cgccgcattt gccagatgaa
    28801 tcagtgcctc tgcccgtttc aacgacatgc ctaacgcttt taatgcctgc gggtcggctg
    28861 ctgccagccg ctgaggcgtg gggaagcaga tatactccgg aaaatcatcc agccgttcgc
    28921 cataaagctg taccactttg gcagtcaatt ttgccgccat cgccacgctc accagttggc
    28981 ctaaaatcgc ccgcacgccc tgctcaaaag catcaacact gccgggtaaa cgcaatccgg
    29041 gccgcgccgc gcctaacttg cccaacgcac cgttaacaat ttgtgggtta cattgcagat
    29101 caaacaggcg gctcattttc gccagacact ctgcggcaac aggttctaaa cctgcactta
    29161 aatttatgtg cagagtatgg cgggctatat ccggaatagc agtcactacg ccgcgatatt
    29221 cgcccaccgc cagactacgg gcataataac tgtccgcgac cgtttccaca ccgctcaccg
    29281 cacgggcggc gagaaatccc aacatccacg accagtcata cggcggctgc cagttcaggg
    29341 tatacatcgc atctccttat tcatccgctt tcagcataaa cgttattcag acgctgcgct
    29401 ttgctttcat attccggttg tcgcgacggc aacatttcgc taaagtcacg ccccttcttc
    29461 accggcatgg ggattatttc gtgtttattg gttttgatta cggtacagca aactgttcag
    29521 tggcggtcat gcgtgacggt aaaccgcact tgctaaaaat ggaaaacgac agcacgctgc
    29581 tgccttcaat gctttgcgcg ccaacgcgtg aggcggtaag cgaatggctg taccgccatc
    29641 atgatgttcc ggcagacgac gatgaaacgc aggcgctgtt gcgtcgggcg attcgttata
    29701 accgcgaaga agatatcgat gttacggcga aaagcgtgca gttcggtctt tcctcactgg
    29761 cacagtacat tgatgatccg gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg
    29821 gtgccagcgg cttaaaaccg cagcgggtag cgctgtttga ggatctggtc tgcgcaatga
    29881 tgttgcacat tcgccagcag gcgcaggcgc agctgccaga agcgattact caggcggtga
    29941 ttggtcgccc gatcaacttc caggggctgg gcggcgatga agcaaacgcc caggcgcaag
    30001 ggattctgga acgcgcggcg aagcgtgccg gattcaggga cgtggtattc cagtacgagc
    30061 cggtagcggc tgggctggat tacgaagcca ccttacagga agaaaaacgg gtactggtgg
    30121 tggatatcgg cggtggtacg actgactgtt cattgctgct gatggggccg cagtggcgtt
    30181 cgcgtctcga tcgtgaagcc agcctgctgg gtcacagtgg ttgccgtatt ggcggtaacg
    30241 atctggatat cgcactggcg tttaaaaacc tgatgccgtt acttggcatg ggtggcgaaa
    30301 ccgaaaaagg catcgccctg ccgattctgc cgtggtggaa tgcggttgcc atcaacgacg
    30361 ttcctgcgca gagtgatttc tacagtagtg ccaacggtcg tctgcttaac gatctggtac
    30421 gcgatgcccg cgaaccggaa aaagtggccc tgttacagaa agtctggcgt cagcgtttaa
    30481 gctatcgcct ggtgcgtagc gcagaagagt gcaaaattgc tctttcaagc gtagcggaaa
    30541 cccgcgcctc actgccgttt atcagcgatg aactggctac gctgattagc cagcaagggc
    30601 tggaaagcgc cctcagccag ccactggcgc ggattctgga acaggtgcaa ctggccctcg
    30661 ataacgccca ggaaaaaccg gacgttatct atctgaccgg cggtagcgcc cgttctccgc
    30721 tgattaaaaa agcgctggaa gaacagttgc cgggcattcc gattgcaggc ggcgatgact
    30781 ttggctccgt caccgccggg ctggcacgct gggcggaagt ggtgtttcgt taattgaaaa
    30841 atggctcagc agcggcttaa atctgcctgc gcctgagcca gtttttctgc cgcctgacgt
    30901 aacagttcca tcaacggcat ggtccgtgct ggagcatgaa ggcggaatcg ctgcaactct
    30961 gtcgcgccga cagtcaacgc cgattccagc cgatgtcgtt tggcggccat tttcgccatt
    31021 accgcttgtc tgtctgcagg cgttaccgct tcgttcggcc tgaacacaaa acggcgttca
    31081 cagctcgctt tccagtcgat gaccgcctgg gtcagatgat caccaaaccc tttaacttgc
    31141 ttaaccccac gacgggtaac atccgctgct gtttcaatac caaaggaccg taacgccgct
    31201 ttacgcgcag ggccaacacc gggaatagag gcaacatcaa taaaaaatcc ctccagaaac
    31261 ttctgcttct gccgttccct tgcagtgtcg tgaagtgctg ccagagcacg tttttcttct
    31321 tccggtaacc cgagcatttc gtccttcatt ttttcgagca tcgtccgttt ggcaataaaa
    31381 ccttccagcc cacccaactg ttggatctgg ctgactaaat ggtcatagtc cattttcgtg
    31441 cgattaaatg cctcacggcg ttgctggatt tctgctttat aagctttgct tgtcagactg
    31501 ccaataatcc agatagccgc cagcacagga accaaaataa tataacgcgg ttctgcctga
    31561 aggccgcaaa gcagcagcga taccgctgac agtgcgatct caagcagaat gatgtattcg
    31621 cgccgtaaca ggcctaaagg aagcggcctg ccagtcggtt ggaaatgatc gggtaatggc
    31681 aattgcactg ctggcggtgc tactgacgcc atcaccatcg cccagacttt cgccagcaca
    31741 aaatccccgc cggtggtaat aacctcttcg ccgagatcaa taaaatagat aacgccttga
    31801 ttatccagcg tacaccacgg gcagtcagtc aaatgagcgg ggtaaacatg cattgctgaa
    31861 acggtacatt tctttaactg ttggcgtaga gagtccagtg ccgctaccca cgcttttgcc
    31921 gtcggacgcc ccgttgccac gccactttcc gtgaatgcct gctgaaacat ggcttcaaca
    31981 tctcccggta acatcgacag cggaatagat cgtggcggcg gttttaaacc acgtcgctga
    32041 ttatctgacg cgtaggcata acggaaatgg gcaatatccg tctccagcgc attacccgca
    32101 tcagagataa gcggcacacc agaataagga tgccgcccac caaacaagac gtgaaaaatc
    32161 agcaacgcaa ggccaaaatt atcgtgattc gcggtacgtt caaaaccgac aaatgatggc
    32221 aaggtttgta gctctggcgg cgtaaaatgc gacacgccga cttcgcataa atgcagtgtg
    32281 ccattggcgt taatctgaaa ggagtcacta tcgatcaaca ccactttgct gtcgcgacct
    32341 accataaagc tgttctggtt tacgtcaccc acgacgtgcc cgtgctcgtg aaccgtagca
    32401 aaagatgaag caatattgcg cgcaacatag agtagaaaat cccacgcaca atgagggtaa
    32461 ctctggcgac gatgtgccgg gctatagatc atatgaatcg gttctttacc agaaactttt
    32521 ggcatcataa aaccgataac ttttccgcct cgtccaccgt gaagcgttgc ctgcggccag
    32581 gcgacataat tcaacaactg cgcgtcagct gtcgcagcca taaaggcaag tttgtcctgt
    32641 tttaaggcgg gtggcggcgt gtgataaatc ttggcgacgc tatcggcaaa ctcattgata
    32701 tcataaaccg caccttcacc gcctttgccc agttcacggc ctgtcgtaac gcactcacca
    32761 gtagcagtat ataaagtggg tttcatggta accacagcgc cagtgccaac gttttatcat
    32821 catcggtacg ctcgtttacc gccgggctgg acaaaaactg ctttaacaat tcaggtaata
    32881 aatcgagctg ttcctgcgtt gctgccgcca gtccattgaa aaaaggggta aagaaaggca
    32941 catgcggaga attatccaac atatttaacg ccagtcgctg gataccatcc gtaaatgctg
    33001 caactttatg cacacgctca gtgctggtga aagtttccag tctggaaacg gcatcttcat
    33061 cggtaatgaa atgcgtcata ttggcatatt cacccgccat cggcgtaagc ggtaattgca
    33121 gaccgtgacc aaaatccacc accacgccgc catcgccaat ctgcataatc aaggtgccgt
    33181 tagccgatga aatcaatccc agaaacgtgc aggcaaaatc gcgaactgct aattctttgg
    33241 cttccgcctc ggcaaaaagt cgctgacgaa cggtcagcac aatatccgtg gcgaggatat
    33301 cattgagtcc aaattcaccg tgctgcactt tttgcgacac ataagccatc gcttcattga
    33361 tggcaagcat cgcgccttcg ccaccctgcg agacactgcc tgcgccatca gcgacaaata
    33421 ccgacaataa tggctgctgg tcatttagcc aggcaacctg catctggcag gcatcctggc
    33481 agggtaaatc agcgctaatg tgcgacgttc caacagcgga tgcataaacc agacgccagc
    33541 tcacactgac gtccagcctt ttggcgcttc cagtacaact tccgttcccg gcgtggagcg
    33601 ggaaaccgaa cgcagtgaac tggataacca gctaaacagt tcgcggaatt gtaatccctg
    33661 cagagacaaa ggctgacgaa cgctgatttg tgccagagtc ttcatatccg ccccctgaac
    33721 accgatggaa aagaaggcaa acttcttatc ttcttccccc tgaaacactt tgttggcggc
    33781 agcctgccac tcatcggttg gagcgccatc ggtgatcagg aaaatccacg ggcgataata
    33841 ggaaatacca tttgcgcggt actcacgttt ccgctcctct accatatcca gggctttggt
    33901 aatggcggca cccattggtg tatcgccctg ggcaaacagg atgggcggga aaaaattagc
    33961 ggcgctggta aagggttgtt ccacatgcac cgggccgaac gtcacaatac caagttccac
    34021 tctttttaag gccagcgggt cggcaagcag ctcatcgcga aaggtaacca atccggcgtt
    34081 aagttcatta atgggtctgc cactcatcga accagaaaca tccagcagca gaatgcaggg
    34141 gcaacgtggt tccgggttac tggcaaaatc gctggtggca aacgtgattt gttcgctcat
    34201 aagaaatcct ttcttagaga attattgagg tccggtatca tactgattgc aataatgaaa
    34261 taagagcaaa cgaagaaact ttcagaatta gtcccggctg cgcagaaaat tttgctattc
    34321 gcctcgcaag aacggagagc cgtcgattga cactaagggc ggagtgacat aatttcagga
    34381 gtgagggtta gggagaggtt tccccctccc cctggtgttc ttagtaagcc tggaagctaa
    34441 tcactaagag tatcaccagt atgatgacgt gcttcatcat aaccctttcc ttattaaaag
    34501 ccctcttctc cgggagaggg tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct
    34561 cctttcgccc tgaatgcagt ctatcgctaa cgcgtgaaca aaatagttcc ctctgtgatt
    34621 attgcgtaac gactcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcag
    34681 attgacaatc ttatcgtgaa ggcatacttt caggagtaag ggtagagcgg ggtttccccc
    34741 gccctggtag tcttagtaag ccgggaagct tatgactaag agcaccacga tgatgatcca
    34801 cttcatcatg accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca
    34861 gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg aattcagtct atcgctaacg
    34921 cgtgaacaaa atagtttcct ctgtgattat tgcgtaacgt ctcgcaaaga atatttgctg
    34981 gcaggatcgc agactgcaaa gcctgcggat tgacattctg aacgtgaagg catactttca
    35041 ggagtgaggg tagagcgggg tttcccccgc cctggtagtt ttagtatgct gggtagctaa
    35101 tcactaagag caccacgatg atgagtatct tcatcatgac cctttcctta tttaagaccc
    35161 cttcctcggg aggggctttc ccgtttcagc atcccgctga aatcgtcggc ttacctcctt
    35221 tcgccatgca tgcagtctat cgctaacgcg tagataaaat agtttcctgt gttattactg
    35281 gatgcgtgct cgcaaatgtg cccgccattc agacgattcc cgacagtgtt tcataattcc
    35341 tccatttttc tcccttattg gctggctaca ctagtatcat tccgcgaaac gtttcaggaa
    35401 gagaaactct taacgatgaa aggcagttat aaatcccgtt gggtaatcgt aatcgtggtg
    35461 gttatcgccg ccatcgccgc attctggttc tggcaaggcc gcaatgaacc ccagagcgca
    35521 gcaccaggtg cgacgaaaca agcgcagcac ccacctgcgg gggggcgccg tggtatgcgt
    35581 tctggccctt tagcgcctgt tcaggcagcg actgccgtgg aacaggcggt tccgcgttat
    35641 ctcaccgggc ttggcaccat caccgcggct aacaccgtta cggtgcgcag tcgcgtggac
    35701 ggccaactga tggcgttgca tttccaggaa ggccagcagg tcaaagcagg cgatttactg
    35761 gcagaaattg accccagcca gtttaaagtt gcattagcac aagcccaggg gcaactggca
    35821 aaagataaag ccacgcttgc caatgcccgc cgtgatctgg cgcgttatca gcaactggca
    35881 aaaaccaatc tcgtatctcg tcaggaactg gatgcccaac aggcgctggt cagtgaaacc
    35941 gaaggcacca ttaaagccga tgaagcgagc gtcgccagcg cacaactgca actcgactgg
    36001 agccggatta ctgcgccagt cgatggtcgt gttggcctga agcaggttga tgttggtaac
    36061 caaatctcca gtggcgacac gaccgggatt gttgtgatca cccagacgca tcctatcgat
    36121 ttgctcttta ctctgccgga aagcgatatc gctaccgtag tgcaggcgca aaaagccgga
    36181 aaaccgctgg tggtagaagc ctgggatcgc accaactcga agaaattaag cgaaggcacg
    36241 ctgttaagtc tggataacca aatcgatgcc actaccggta cgattaaagt gaaagcacgc
    36301 tttaataatc aggatgatgc gctgtttccc aatcagtttg ttaacgcgcg catgttagtc
    36361 gacaccgaac aaaacgccgt ggtgatcccc accgccgccc tgcaaatggg caacgaaggc
    36421 cattttgtct gggtactgaa tagcgaaaac aaagtcagca aacacctggt gacgcctggc
    36481 attcaggaca gccagaaagt ggtgatccgc gcaggtattt ctgcgggcga tcgcgtggtg
    36541 acagacggca ttgatcgcct gaccgaaggg gcgaaagtgg aagtggtaga agcccagagc
    36601 gccaccgcac cggaagagaa agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc
    36661 tgatgcaggt gttacccccg agcagcacag gcggcccgtc gcgcctgttt attatgcgtc
    36721 ctgtggccac cacgctgctg atggtggcga tcttactcgc cgggattatc ggttatcgcg
    36781 ccctgcccgt ttcggcgctg ccggaagtgg actatccgac cattcaggtg gtcacgctct
    36841 acccaggtgc gagcccggat gtcatgacct ctgccgttac cgcgccgctc gaacgccagt
    36901 tcgggcagat gtctggtctg aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta
    36961 tcactttgca gttccagcta acattgccgc tggatgtcgc cgagcaggaa gttcaggccg
    37021 cgattaacgc cgccactaac ctgctaccta gcgatctgcc taacccgccg gtttacagca
    37081 aagtgaaccc ggcagatccg ccgattatga cgctcgccgt cacctcaacc gccatgccga
    37141 tgacccaggt agaagatatg gtggaaaccc gcgtcgcgca gaagatctcg cagatttccg
    37201 gcgtcggcct ggtgacactt tccggcggcc agcgtccggc tgttcgcgtc aaacttaacg
    37261 ctcaggcgat tgccgccctc ggcctgacca gcgaaaccgt gcgcaccgcc attaccggtg
    37321 ctaacgttaa ctcggcaaaa ggtagcctcg acggcccttc ccgcgcggtc acgctttccg
    37381 cgaatgacca gatgcaatcc gccgaagagt atcgccagtt gattatcgcc taccagaacg
    37441 gcgcgccgat tcgtctgggc gatgtcgcaa ccgtagagca aggggcagaa aacagctggc
    37501 tcggcgcgtg ggcgaacaaa gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg
    37561 ctaacattat ctccaccgcc gacagcatcc ggcagatgct gccgcagctt accgagagtc
    37621 tgccgaaatc ggtgaaggtg acggtgcttt ctgaccgcac caccaatatc cgagcatccg
    37681 tcgacgatac ccagtttgaa ttgatgatgg ctatcgcgct ggtagtcatg attatctacc
    37741 tgtttttgcg caatattccg gcgaccatca ttcccggtgt cgctgtgccg ctgtcattaa
    37801 tcggcacctt cgcggttatg gtgtttctcg atttttcaat caataacctg acgctgatgg
    37861 cgttaactat cgccaccggg ttcgtggtcg atgacgccat cgtagttatc gaaaatattt
    37921 cccgctatat cgaaaaaggc gaaaaaccgt tggcggcggc gctcaaaggc gcgggtgaaa
    37981 tcggctttac cattatctcg ctgaccttct cactgattgc ggtgttgatc ccactgctgt
    38041 ttatgggcga tatcgtcggg cgactgttcc gcgaatttgc tattaccctg gcggtagcga
    38101 ttttaatctc agccgtggtg tcgctaaccc tgacaccgat gatgtgcgca cggatgctca
    38161 gccaggagtc gttgcgtaaa cagaaccgct tctcccgtgc ctcggaaaaa atattcgaca
    38221 ggataatcgc cgcctatggt cgtggactgg cgaaagtgct gaatcatccg tggctgacct
    38281 taagcgtggc gctcagcacg ctgctgctta gcgttctatt gtgggtgttc attccgaaag
    38341 gtttcttccc ggtacaggat aacggcatta ttcagggcac tttgcaggca ccgcaatcca
    38401 gctcctttgc caatatggcc cagcggcaac gccaggtcgc ggacgtgatt ttgcaggatc
    38461 cggcagtgca aagtctgacc tcatttgttg gcgttgatgg cactaacccg tcgctgaaca
    38521 gtgcgcgttt gcagatcaac ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag
    38581 tcatcgcccg tctgcaaacg gcggtggata aagtgccggg cgtcgatctc ttcctgcaac
    38641 caacgcagga cctgaccatc gatactcagg tcagccgcac ccagtaccag tttaccttgc
    38701 aggccacgtc actggatgcg ctcagtacct gggtgccaca gttgatggaa aaactccagc
    38761 agctgccaca gctttctgat gtctccagcg actggcagga caaagggctg gtggcgcatg
    38821 tcaatgttga tcgcgacagc gccagccgtc tggggatcag catggcggac gtcgataacg
    38881 ccctgtacaa cgcgtttggt cagcggctga tttccactat ttatactcag gctaaccagt
    38941 atcgcgtggt gctggagcac aacaccgaaa ataccccagg cctcgcggcg ctggatacca
    39001 ttcgcctgac cagcagcgac ggcggcgtgg tgccgctaag ctcaattgcc aaaattgagc
    39061 agcgttttgc gccgctttcc atcaaccatc tggatcagtt cccggtaacg accatctcct
    39121 ttaacgtgcc ggataactat tcgctgggcg atgcggtgca ggcaattatg gacaccgaga
    39181 agacgctgaa tctcccggtg gatatcacca cgcaattcca gggcagcacc ctcgccttcc
    39241 agtcggcgct gggcagcact gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg
    39301 tgctcggcat tctgtacgag agctttattc acccgatcac cattctctcg acgctaccca
    39361 ccgcaggggt cggcgcactg ctggcattaa tgcttgccgg aagtgagctg gatgtgattg
    39421 cgattatcgg cattattttg ctgatcggta tcgtgaagaa gaacgccatc atgatgatcg
    39481 acttcgcgct ggctgctgag cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg
    39541 cttgtctgtt gcgttttcgt ccgatcctga tgaccaccct ggcggctctg cttggcgcgc
    39601 tgccgctgat gttgagtacc ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca
    39661 tggtcggcgg cctgattgtc agccaggtgc tgacgctgtt caccacgccg gtgatttatc
    39721 tgctgtttga ccgcctggca ttgtggacca aaagtcgctt tgctcgccat gaagaggagg
    39781 cgtaagtgaa gttttttgcc ctcttcattt accgcccggt ggcgacgatt ttactgtcgg
    39841 ttgccattac cctgtgcggc atactgggtt tccgtatgct gccggtcgcc ccgctgccgc
    39901 aggtcgattt tccggtgatt atggtcagcg cctcgctgcc cggtgcatcg ccggagacaa
    39961 tggcgtcttc cgttgccacg ccgctggagc gctcacttgg gcgcattgcc ggggtcagtg
    40021 aaatgacctc cagtagttcg ctcggcagca cgcgcattat tttgcagttt gattttgacc
    40081 gggatatcaa cggcgcagcg cgtgatgtgc aggcggcgat caacgctgca caaagtttgc
    40141 tgcccagtgg gatgcccagc cgtccgacct atcgcaaagc gaacccgtcg gatgcgccaa
    40201 ttatgatcct gacgctaacc tccgatactt attcgcaggg tgaattgtac gatttcgcct
    40261 cgacgcagct ggctccgacg atttcgcaaa tcgacggcgt cggtgatgtc gatgtcggcg
    40321 gcagctcgct gcccgccgtg cgcgtcgggc tgaacccgca ggcgctgttt aatcagggcg
    40381 tgtcgctgga cgacgtacgc actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg
    40441 cgctggaaga tggcactcac cgctggcaga tccagaccaa cgatgaactg aaaaccgccg
    40501 ctgaatatca gccgctgatt attcactaca acaacggcgg cgcggttcgt ctgggcgatg
    40561 tggcgacggt gaccgactca gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac
    40621 cggctatttt gctgatgatc cgcaagctgc cggaagccaa tattatccag acggttgaca
    40681 gcatccgggc aaaattaccg gagttgcagg aaaccattcc ggcggcgatt gatctgcaaa
    40741 ttgctcagga tcgctccccc accattcgcg cctcgctgga agaagtcgag caaacgctga
    40801 ttatctcggt ggcgctggtg attctggtgg tctttttatt cctgcgctcg ggtcgcgcca
    40861 ctattattcc cgccgttgcg gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc
    40921 tgtgcggatt cagtctcaat aacctgtcgt taatggcgct caccatcgct actggtttcg
    40981 tggtggatga cgccatcgtg gtgctggaaa acattgcccg tcatctggaa gcggggatga
    41041 aaccactaca ggccgcgcta caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga
    41101 gtctgtcact ggtggcggtg ttcctaccgc tgctgttgat gggcggattg ccgggccgac
    41161 tgttacgcga gtttgccgtg acgctttctg tcgccattgg tatttcgttg ctggtttctc
    41221 tgacattaac gccaatgatg tgtggctgga tgctgaaagc cagcaagccg cgcgagcaaa
    41281 agcgactgcg tggttttggt cgcatgttgg tagccctgca acaaggctat ggtaagtcac
    41341 tgaaatgggt gctcaatcat acccgtctgg tgggcgtggt gctgcttggc accattgctt
    41401 tgaatatctg gctatatatc tcgatcccga aaaccttctt cccggagcag gacactggag
    41461 tgttgatggg cgggatacag gcggatcaga gtatttcgtt tcaggcgatg cgcggtaagt
    41521 tgcaggattt tatgaaaatt atccgtgacg atccggcagt ggataatgtc accggcttta
    41581 ccggcggttc acgagttaac agtgggatga tgtttatcac cctcaagcca cgcgacgaac
    41641 gcagcgaaac ggcgcagcaa attatcgacc gtctgcgggt gaagctggcg aaagaaccgg
    41701 gggcgaatct gttcctgatg gcggtacagg atattcgcgt tggcggacgt cagtctaacg
    41761 ccagttatca gtacacgttg ttatccgacg acctggcggc actgcgcgaa tgggagccga
    41821 aaatccgcaa aaaactggcg acattgccgg aactggcgga cgtgaactcc gaccagcagg
    41881 ataacggcgc ggagatgaat ctggtttacg accgcgacac catggcacgg ctgggaatcg
    41941 acgtacaggc cgccaacagc ctgttaaata acgccttcgg tcagcggcaa atctcgacca
    42001 tttaccagcc gatgaaccag tacaaagtgg tgatggaagt ggatccgcgc tatacccagg
    42061 acatcagcgc gctggaaaaa atgttcgtta tcaataacga aggaaaagcg atcccgctgt
    42121 cgtatttcgc taaatggcaa ccggcgaatg ccccactatc ggtgaatcat cagggattat
    42181 cggcagcctc gaccatctcg ttcaacctgc cgaccggaaa atcgctctcg gacgccagtg
    42241 cggcgatcga tcgcgcaatg acccagcttg gtgtgccttc gacggtgcgc ggcagttttg
    42301 ccggcacggc gcaggtgttc caggagacga tgaactcaca ggtgatcctg attatcgccg
    42361 ccatcgccac ggtgtatatc gtgctgggta tcctttacga gagttatgta catccgctga
    42421 cgattctctc caccctgccc tcggcgggcg ttggggcgct gctggcgctg gagctgttca
    42481 atgccccgtt cagcctaatc gccctgatag ggatcatgct attaatcggc attgtgaaga
    42541 aaaacgccat tatgatggtc gattttgcgc ttgaagccca acggcacggt aacctgacgc
    42601 cgcaggaagc tattttccag gcctgtctgc tgcgttttcg cccgattatg atgactaccc
    42661 tggcggcgct gtttggcgcg ttgccgctgg tgttgtcggg cggcgacggc tcggagctgc
    42721 gccaacccct ggggatcacc attgtcggcg gtctggtaat gagccagctc ctgacgctgt
    42781 ataccacgcc ggtggtgtat ctctttttcg accgtctacg gctgcgtttt tcgcgtaaac
    42841 ctaaacaaac ggtaaccgag taaatgacag atcttcccga cagcacccgc tggcaattgt
    42901 ggattgtggc tttcggcttc tttatgcagt cgctggacac caccatcgtt aacaccgcgc
    42961 ttccctcaat ggcgcaaagc ctgggggaaa gtccgttgca tatgcacatg gttattgtct
    43021 cttatgtgct gaccgtggcg gtgatgctgc ctgccagcgg ctggctggcg gacaaagtcg
    43081 gcgtgcgcaa tattttcttt accgctatcg tgctgtttac tctcggttca ctgttttgcg
    43141 cgctttccgg cacgctgaac gaactgttgc tggcacgcgc gttacagggc gttggcggtg
    43201 cgatgatggt gccgatcgga aggcttacgg tgatgaaaat cgtaccgcgc gagcaatata
    43261 tggcggcgat gacctttgtc acgttacccg gtcaggtcgg tccgctgctc ggtccggcgc
    43321 ttggcggcct gctggtggag tacgcgtcgt ggcactggat ctttttgatc aacattccgg
    43381 tgggaattat tggtgcgatc gccacattga tgttaatgcc gaactatacc atgcagacgc
    43441 ggcgctttga tctctccggc tttctgttgc tggcggttgg catggcggta ttaactctgg
    43501 cgctggacgg cagtaaaggt acaggtttat cgccgctggc gattgctggc ctggtcgcag
    43561 ttggcgtggt ggcactggtg ctttatctgc tgcacgccag aaataacaac cgcgctcttt
    43621 tcagtctgaa actgttccgc actcgtacct tttcgctggg cctggcgggg agctttgccg
    43681 gacgtatcgg tagcggcatg ttgcccttta tgacgccggt tttcctgcaa attggccttg
    43741 gcttctcacc gtttcatgcc ggactgatga tgatcccgat ggtgcttggc agcatgggaa
    43801 tgaagcgaat tgtggtacag gtggtgaatc gctttggtta tcgtcgggta ctggtagcga
    43861 ccacgctggg tctgtcgctg gtcaccctgt tgtttatgac taccgctctg ttgggctggt
    43921 actacgtttt gccgttcgtc ctgtttttac aagggatggt caactcgacc cgtttctcct
    43981 ccatgaacac cctgacgctg aaagatctcc cggacaatct ggcgagcagc ggcaacagtc
    44041 tgctgtcgat gattatgcaa ttgtcgatga gtatcggcgt caccatcgcc gggctgttgc
    44101 tgggactttt tggttcgcag catgtcagcg tcgacagcgg caccacacaa accgtcttta
    44161 tgtacacctg gcttagcatg gcgtttatca tcgcccttcc ggcgttcatc tttgccagag
    44221 tgccgaacga tacgcatcaa aatgtagcta tttcgcggcg aaaaaggagc gcgcaatgaa
    44281 gttctggcga cccggtatta ccggcaaact gtttctggcg attttcgcca cctgcattgt
    44341 cttgctgatc agtatgcact gggcggtgcg tattagtttt gagcgtggct ttattgatta
    44401 catcaagcat ggtaatgaac agcgattaca actgttaagt gatgcgcttg gcgagcagta
    44461 tgcgcagcat ggcaactggc gcttcctgcg caacaatgat cgctttgtct ttcagatcct
    44521 gcgttcattt gaacacgata attcggaaga taaacccggc ccgggtatgc caccgcacgg
    44581 ctggcgtacc cagttctggg tggttgatca aaacaacaaa gtgctggttg gtccgcgagc
    44641 gccgattcca cctgacggta cacggcgacc cattctggtc aacggtgcgg aagttggcgc
    44701 ggtgatcgcc tcccccgttg agcggttaac gcgcaatact gatatcaatt tcgataaaca
    44761 acagcggcaa accagctggc tgattgtcgc cctggcaacg ttactcgcgg cgctcgccac
    44821 ctttctgctg gcgcgtggtc ttttggcccc ggtcaaacgg ctggtggacg gcacgcacaa
    44881 actggcggcg ggcgatttca ctacccgcgt aacgcccacc agtgaagatg aactgggcaa
    44941 actggcgcaa gacttcaacc agcttgccag cacgctggag aaaaaccagc aaatgcggcg
    45001 cgattttatg gccgatattt ctcacgaact gcgtacgcca ttagcggtgc tgcgcggtga
    45061 actagaagct attcaggatg gcgtgcgcaa attcacgccg gagacggtgg cgtctttaca
    45121 ggcggaggtc ggtacactga ccaaactggt tgacgatctt catcagttgt cgatgtctga
    45181 tgaaggcgct ctcgcctatc aaaaagcacc ggtagatttg atcccactgc tggaagtggc
    45241 gggcggcgca tttcgcgaac gattcgccag ccgtggcctg aaactgcaat tttccctgcc
    45301 agacagtatt accgtatttg gcgatcgcga ccgtttaatg cagttgttca ataacttact
    45361 ggaaaacagc ctgcgctaca ctgacagcgg cggcagcctg aaaatctctg ccgagcagca
    45421 cgacaaaacg gtgcgcctga cctttgccga cagtgcgcca ggtgtcagtg acgatcagct
    45481 acaaaaattg tttgaacgtt tttatcgcac cgaaggttcc cgcaaccgtg ccagcggcgg
    45541 ttccgggctg gggctggcga tttgcctgaa cattgttgaa gcacataatg gccgcattat
    45601 tgccgcccat tcgccttttg gcggggtaag cattacagta gagttaccgc tggaacggga
    45661 tttacagaga gaagtatgac cgagttacca atcgacgaaa acacaccgcg tattttgatc
    45721 gtggaagatg aaccgaagct ggggcagttg ctcattgatt atctgcgtgc tgcgagctat
    45781 gcgccgacgc ttatcagtca cggcgatcag gtactgccgt atgtgcgcca gacaccaccg
    45841 gatctgatcc tgttggatct gatgctccct ggcaccgatg gcctgacgct gtgccgggaa
    45901 attcgtcgtt tttctgacat tcctatcgtg atggtgacag caaaaatcga agagatcgac
    45961 cgcctgctgg ggctggagat tggcgcagat gactacatct gcaagccgta cagcccacgg
    46021 gaagtggtag cgcgtgtcaa aaccattttg cgccgttgca aaccgcagcg tgagttgcaa
    46081 caacaggatg ctgaaagccc gttgattatc gacgaaggcc gttttcaggc ttcatggcgc
    46141 ggtaaaatgc ttgacctgac gcctgcggaa tttcgtctgc tgaaaacgct ctctcatgaa
    46201 ccaggaaaag tgttctcccg cgagcaattg ctcaatcatc tttatgacga ctaccgcgta
    46261 gtaaccgacc gcaccatcga cagccacatt aaaaacctgc gccgcaagct ggaatctctc
    46321 gacgccgaac agtcatttat ccgcgccgtt tacggcgttg gttaccgctg ggaagccgac
    46381 gcctgccgca tcgtttagtt ttagcgacat gactttgtta gccggagatg gcgttcagac
    46441 ccgatccggc aacattatcc cacgcatggt cagcaaactg acattatctc tccgccctgg
    46501 caggcatcag aaaatcgctc atactttaat cggtaaacag cacctttaga tgctgttttc
    46561 gatacacaat ttcaatcaag gagtcattat ggctggttgg tttgaactca gtaagagcag
    46621 tgataatcag ttccggtttg tgctaaaagc gggcaatggt gagactatcc tcaccagcga
    46681 gctgtatact tcaaaagcct ctgcggaaaa gggcatcgcg tcggtgcgta gcaacagccc
    46741 gcaagaagaa cgctatgaga aaaaaacggc aagcaacggc aaattctatt tcaatctgaa
    46801 agccgccaat catcaaatta tcggctccag ccagatgtat gccaccgcgc aatctcgtga
    46861 aaccggaatt gcctccgtta aagccaatgg cacaagccag acggtgaaag acaatacgta
    46921 attacgatgc cgggcgtggg ttgttggcgt ccggctttat ttccaaataa cgcttattta
    46981 tttacctgcg ccattaaaat agtggacact gatgaatact cattgtgctt tctttgttta
    47041 acatctccat gcttatcctc ctgaacatgt tcatctgcca aaatatcttc taatactttt
    47101 cgtgacgcta tccggtttat gacatacact gatgtaagat ttttacttac aggagagcat
    47161 aaacatggcc agaacgatga cggttgatct cggcgatgag ttgcgtgaat ttatcgagtc
    47221 cttaattgaa tccggcgact atcgcaccca aagcgaagtg atccgtgagt cactgcgctt
    47281 acttcgagaa aagcaagcag agtctcgcct gcaagcatta cgtgacatgc ttgcagaagg
    47341 gttgagtagt ggtgaagctc agccgtggga aaaggacgcg tttttacgga aggtgaaagc
    47401 agggataagg aaataatgtg cgtattatta aactcatgcc aaaagccaat gaggatttag
    47461 aaggcatctg gtattatagt tatcaccatt tcggcgaacc acaagccgac agatatgtgg
    47521 aacatctttc agacgttctc cagattctga gtaacaataa tataggtacg cccagacctg
    47581 aattaggcga aggtattttt gttcttcctt ttgaacgcca cgtaatttat ttcctgcaat
    47641 caccagggga aattatcgtc attcgtatcc ttaaccaaaa ccaggacgcc accagacatt
    47701 tacactggag ctaaacccgc cgattgcgct acaatgcccg cccttaaagt gggggcactc
    47761 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    47821 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    47881 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctgcgtgtg cgcaacaacg
    47941 aattcaacca cgaaaacctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    48001 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    48061 tgaaaccagt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    48121 tgctggtgcg tgagcacttc cctgaaatgc cgatccatct ctcggtacag gctaacgccg
    48181 tgaactgggc gacggtgaaa ttctggcagc aaatgggcct gacccgcgtg atcctctctc
    48241 gcgagctgtc gctggaagag attgaagaga tccgcaatca ggtgccggat atggagatcg
    48301 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    48361 atatcaacaa gcgcgatccg aaccagggta cctgcaccaa cgcctgccgc tgggagtaca
    48421 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    48481 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    48541 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    48601 acatcatgaa ctcgaaagat ctgcgcgcta tcgcccatgt tgaacgcctg accaaaatgg
    48661 gcgtgcattc gctgaaaatc gaaggccgta ccaaatcttt ctactattgc gcacgcaccg
    48721 cacaggttta ccgcaaagct attgatgacg ccgctgcggg caaaccgttt gacaccagcc
    48781 tgctggaaac cctggaaggc ctggcgcatc gcggttacac cgaaggattc ctgcgtcgtc
    48841 atactcacga cgattatcag aactacgaat acggttattc ggtttctgac cgccagcagt
    48901 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc ggcggtagcg gtgaaaaata
    48961 aattctccgt tggcgacagc cttgagctga tgacgccgca aggcaacatt aactttaccc
    49021 ttgagcacat ggaaaacgcc aaaggtgaag cgatgccggt cgcaccaggc gatggttata
    49081 ctgtgtggct cccggtgccg caggatcttg agctcaatta cgcgctgctg atgcgtaatt
    49141 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    49201 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    49261 tcgctgaaaa acataaccca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    49321 gtaatctccc ttacacgggc ttatttttta tgcataagcc ctatccctgg tcaccgtctt
    49381 ccattgacca catcgataga atctcccatc atagcacgat gcctttcacg taacggcatc
    49441 gtgctcgcac aggttccggc tacgcacagc cagaacgcgc atgtttgacg cttaccaaaa
    49501 aatattctca ctctacacat ttgaatgtca gacgagcgac gccatgtaat cctgcacctt
    49561 ctgtcttcag gtcaactatc tgcatttttt tgccctgagt aacacagaaa tgggctgcat
    49621 cattttttac tatattttct gcaccagata ttctccccct ggctaaagaa gcttcggctt
    49681 cggtgtagta ttggttatcg agtttacgct gaatattact tttatatgca agaccaaatt
    49741 taccgatact tgtctcatca ttatgcacag cacaaccaga cataagaaaa atactaaata
    49801 atgatatagc aactatcttt ttcatctcac ctttccccat taaataccaa cgacactctc
    49861 tagtgtttaa atataataat ggctgattat tataattgaa taggattata ataaatgttc
    49921 tgtacaacat ttcctacata agtaggaatt acggacatgg aggcccttcc gggtaactcc
    49981 atgggggctt taatatatta tattgaagat gccactgttt agttgaatat taggtatatg
    50041 ctcttttttg acatttatcg gtggctgccg ttagtattcg ctgtcccaat tgcaagctcc
    50101 tggtggtaac cactgaatcc tccatacttg aactgacttt ttatcctccg actttcatcc
    50161 tgttctgact ccaccttttc ttttctgctc tacactatct acagaccaat cataaaggca
    50221 catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa aagtactgac
    50281 aatttaccat tgcgcgaagc aattgtgctg ttgcgtgagg aaggaatgac gatccatgtg
    50341 cgggtcacct gggagaaagg cgatgccgca cgatatgtag aggaggcccg gaagttgggc
    50401 gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta atgaagtttc tacggcgttg
    50461 attcagtgtg agggggataa catacccgcg ctgggaattt tgccattagg aaccgccaat
    50521 gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa actggcaatt
    50581 gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg ttttattaat
    50641 atggcgacag gcgggtttgg gacgcgtatt accacagaaa cgccggaaaa attaaaagcc
    50701 gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac tctgcaaccg
    50761 gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct ggtcattggt
    50821 attggtaacg ggcgtcaggc cggtggcggt cagcaattgt gtccgaacgc gttaattaac
    50881 gatggcttgc tgcaactgcg catttttacc ggcgatgaaa ttcttccagc tctcgtatca
    50941 accttaaaat ctgacgaaga taacccgaat attatcgaag gcgcttcgtc gtggtttgat
    51001 atacaagccc cacacgaaat cacttttaat cttgatggcg aaccgttgag tgggcaaaac
    51061 tttcatattg aaatacttcc ggcggcgttg cgttgtcgat taccaccaga ttgcccatta
    51121 ttgcgttaat caaactattc tctcttgaac cagagagaat ccgtaatgcc ggatgcggtg
    51181 taaacacctt atccggcata cagaacaata ctacaccgta attaatttta cccgtgtctt
    51241 ctgcaatgcc agtttatcca cctctgacac agcatcatcg gtaataataa tgtcaatttt
    51301 ttcgattggc agtacctgat taaaaccgcg acgattaaat ttcgatgaat cgagaacggc
    51361 aatcacttta ttagcagcgg tgaccatcgc accgctaatc gaataacctt cattaaaggt
    51421 cgtaatacca ttaaccgcat caataccatc agcaccgaca aacattaaat cggcattaat
    51481 atcctgcaat gaacgctcgg caatggaacc atgcatcgag cgcgttttat ggcggaccgt
    51541 gccaccgcag acgaccagag taatgtcttt attttcggaa agggcaaacg ctgccgggag
    51601 actgttggta atcaccgtga tatttttggc ggtcattaat ccttcggcga taagcattgt
    51661 cgtacttccg ctgtcgagaa taacggtcat cccttcgtgg atcattttga ctgccgcctg
    51721 cgctattctg ttttttggcg cgctggcgag ctgaaatcgc tctttaaacc cgacttcctg
    51781 ggtctcggtt tcactattac cggacattat tttcgccgca ccgccatgaa agcgcgtaac
    51841 cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt gtcgcttccg aggcagcaaa
    51901 tactcccgcc agatcctgaa caagcacggt tccctgttca ttcactaatt ggatgatctt
    51961 attccttcgc tcgaatgaat tcatcgtcat ttacccatat tcaattgtgg ctagtgtaaa
    52021 cgaaggggga ccattggtga atgatctgtt ttgcacaata ccgggccagc atcacgctgg
    52081 cccgcggttt cagggaatga gcaacacttt gcccggcata gcattacgag cgatgtcacg
    52141 caccgcctgg gcgaagcttt caaagcttcc acggtgaacg attaatggct ctaggcttaa
    52201 cttacgttct gtcagcaacc ggctcgccgt ttcccactcc tgccccggcc aagggctgga
    52261 gtagttcatc caactgccga taaccgtcag ctctttacgc aatattttgc caaacgttgc
    52321 cgatgttaaa tgcagatcct gatgcaacgt ccccaccagc gccagttggg catgaggacc
    52381 ggcaatctct accgccagtt cgacagtttg cggtacgcca gccgtctcga ggataagctg
    52441 attaaagcgc agctcgcgta aaacgccctg catttgcggc gcgctcattt cgctactgtt
    52501 aaatgtttgc atcgcaccga aagattttgc cagtgctagt ttttctgagc taatgtcgat
    52561 cgccgtcaca ctctttgctc ccagcgcgac agcgcactga atagccagca ggccaatggt
    52621 tccggcacca ataataataa cgtttttatt ctcacaacct tgcgctaaat gaaaagcatg
    52681 caggccaacg gtaatcggtt caataaaagc cccatcctca ataggcatat ccgtgggtag
    52741 agcaaagaca tttttccgct taacgacaat atattcggca aatccaccat cacgccgtga
    52801 gccaataaaa tcatattttg cgcactggga ataaaaccct ttcagacact ctggacaagt
    52861 aaaacagggt aataacggca cacaggcaac cgcatcgcca gggtgtaaat catcaacacc
    52921 ggatcctacc gcatcaatat agccgctaaa ttcatggcct aacgttattg gataataatg
    52981 tgcaccattt ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc tggcaatttt
    53041 tacccgcacc tcatcctgat gtttaatttc aggaatgacg ctttctgcaa cgcgcacgat
    53101 accatcagta tcattcacca ctgatttcat aaaaactcct gattgttaag ggggataacc
    53161 tccccctcag gtaaaaatta ttctgcaaga acgactttct cttgtttaat aaagccacgc
    53221 gctctacgcc aggtcatgaa aataccggta agataaattg cgccgataat aatgaaaccg
    53281 ggaatatttt gcggggagaa aacctgaatc agtaaccagg taatgggaga accgccctga
    53341 tccattgaag ccaccatacc cccggctttt aacgccccag cattagccgc cagttgggtg
    53401 tgcaaaccaa tagtttgcgt cgcgatccac agggtgatgc tcataatgat gacacccgag
    53461 attaaggtgc ggaacagatt tccacgatgc accgcgaccg ccatcgccac aaagaagccg
    53521 atagtggcaa gatcgccaaa cggcagcacc tgattacccg gcacacaaac agcaattaaa
    53581 atggtgagtg gaataaaaat caggcttgcc gataccaccg ccgtgtgccc cagcagcaac
    53641 gccggatcaa ggccaatcag gaactcctga ccgccgaact tcgcctgtaa acgactacgg
    53701 gcctgcttag cgatgggtgt taaaccatcc atgatgggtt aaaccatcca tgatgggttt
    53761 aatcacccgt ggcatcagca gcatcaccgc cgctgttttt accgccagct gcaatacacc
    53821 tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac ccacggtgac
    53881 aggctcgcca aacgggccaa atttgcgctg aatatcgtcg gcgctaaatt taatacggtt
    53941 aacgcctggg attttctcga tgatagcatc gaccagcacc gcaattggcc ccatatacgc
    54001 cgacgtaccg tgcgggattg caatgccttc cagctcaaag aaatttcggg tatcgcgggc
    54061 aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt accacacccg ccatccctat
    54121 catccatgaa ccggttgcca gatgcagcaa cgcgccggtg aaggtcatat gccagatatt
    54181 ccagatatca acatttacca cccgcgtcat acgggtcagt aacatcgcca cgttaaccag
    54241 aatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg gtgaagagcc
    54301 cggccagccg acatcgacca catgcagatt caggtcgaaa ttttccgcca tcgctttcgc
    54361 cgccgggcca atggaatcca gcattaagcc aatcacaagg ccaatgccaa caaagccaat
    54421 cccgatatgc agacccgctt taaagcaatc gcctgccttc atgcctaaaa ttttagaaaa
    54481 aataatgatg acaatcggca acatcaccgt agggccgaga tcgagaatat aacgcatgac
    54541 ttctgaaaac ataggtcacc cctgtaagat agtcagaatt ttattttgta atgcttcgat
    54601 accgacacca gaaacaaaag gcatgccgtg aactaatgga atatcgccaa aactacgatc
    54661 cactttggca gtggtgcata tcaaatgcac gccatccata taggtttcta tttcattaac
    54721 ccgacactgg attaattcaa caggaatatt atgactctga cacaactctt taatttcttc
    54781 cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg acaataatct tgcgtttcat
    54841 aaatagtcct ttttattgtt acagagggat ttattcgtct gaatctaaaa catactttgt
    54901 gaagtattcc tttaactgag tttcaggaag agtgattagt gtctcgacga tatcgggctg
    54961 ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc tgctgcggat tttccacaat
    55021 caacgcaata accaacgata ccgccacgtc attatcatca tccgcttgct gaaaatgaac
    55081 tttatttgtt ggccttaaca gataaatggc tgacgactta gcatgaatcg cctcacaatg
    55141 cggtattgca atggcgtgct gctcaagcat tatcccagta gggaattctg cttctctggc
    55201 aattaatgcc tgtggccagg tatcatgaac cacacctttc gcgagcatct cattaccgat
    55261 atgggttaaa acttcgctac gatcgacaaa agaaattccg ctacgaacaa acaggttagt
    55321 catataccgt ccttattccg cacagccgta gcgataggtg cgcaaaacat cataaatttt
    55381 atccataata agctgatgcg gtattgctga taattccccg gactgaatgc gttcaaattg
    55441 tttaggaaga tactgactaa tcatgcccag tgggatgtcc acgccttcca gattcaccat
    55501 catcgtttca acgctatttt taatccgact atgcggccag taataacgaa tacgatccga
    55561 taggctgtaa cgaatatcca gtaatgaatc gttaaaaccc gtgcgataat attttttcca
    55621 gtattgcggt tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc tgcgattttc
    55681 gggggcgata agttcctgct caatttgtgc cagtgcaaat atcgcctcgc gtaaagcaaa
    55741 ggttaatgcg ggaccgactt tcaatattgc aaagtgatcg cggactaatt cccaataagc
    55801 cgtccgggtc tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt tttctatcca
    55861 ttgcgccagc gcctgcgctt cctgcggctg ataatggata atattgctgt gatcaaattc
    55921 cacacccggc tgcaccacga tggcaatcac acgtgttaac gcctctgtca ggccacgggc
    55981 aataaaggcc ttttgatgcg tacgtaaagt attggcggca tcttcaacat gggtgatgtg
    56041 tactgactga atggcgctgg cctcaccgcc cggaaccgga acttcggtgc caatgacata
    56101 gctcagttgc tcacgctggc aatctgtcgc cacactttcc gcagcaaagc aaagcacagc
    56161 agctcgttcc gcaaccgttt ctggtgctaa cggtatggga tcccccgcgc aggacattga
    56221 cgcatcaaga tgaattttac tgaagccagc acgaacatat gcctttacca gctcgacgga
    56281 tttttccatt gccgcatccg cattttcttg ctgccagcag tttggcccca gatggtctcc
    56341 gccgagaata atgcgctcgc gcgcaaatcc gactttatcg gcaatcgtaa aaacaaattc
    56401 gcgaaagtct gccggtgtca ttccggtata accgccaaat tgattgacct ggtttgacgt
    56461 tgcttcaatc agcactttgc gcgtgctgtt gcgatcaaat gccagcgccg cttcgataac
    56521 caacggatgg gcagaacaga ctgaacatat gccgatatgt tcaccagctt tatgccgggc
    56581 aattaacgtt ttcatgtttt ttccttgtta aatggcaggt gcgttatgcc ctgccctcgc
    56641 agccacaatc ggcaatcact ttgctcacca catcgcgcat tgcggattta gccgactgca
    56701 aataatcccg gggatcggtc gcttcggggt gctcggtcag gtattttttt aacgcctgcg
    56761 agaaggcatt tttcagctcc gttgcaacgt tgattttgca tatccccagt ttgatggttt
    56821 gctgaatatc cttagtcgat aaccctgacg cgccatgcag caccagcggt aagttcaccc
    56881 actggcgaat gttctccagt cgagaaaaat caagcgccgg tgcgctggca tacataccat
    56941 gagccgtgcc gatggcgacc gccagggaat caattccggt tgcctcggca aattcacgcg
    57001 cctgagcggg gttggtataa aaagcatcgg cttcattgac ttgcacatca tcttcctggc
    57061 cgccaagttg ccccagctcc gcttcgacgc tgacatcaaa gcgatggcaa aaatccacca
    57121 cctctttgac ccgtgaaata ttttgcgcaa aaggcaaatg cgaggcgtca atcatgactg
    57181 agcgcacgcc agaacgaacc ttctgagcga tatcgtcaaa tttcgtgtga tggtcgagat
    57241 gaattgccag tggatggtga tattgcttcg ccatcgcgct gaccagcgcc aagagatttt
    57301 ctgtaccagc atgagtaaat gtgccaggcg ttccggcgat gatgaccggc gcatgcaggt
    57361 tggcagcggt ttctaccacc acttgcatcg tttcgagatt gtgaatattg aatgccggaa
    57421 ccgcataacc gccgcgctgt gcgttgttca gcatctgctt tgtcgatacc acg
//
LOCUS       DAZGCQ010000017        57352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000017 DAZGCQ010000000
VERSION     DAZGCQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.017"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            277..336
                     /gene="ibsC"
                     /locus_tag="ACHK07_001139"
     CDS             277..336
                     /gene="ibsC"
                     /locus_tag="ACHK07_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO8101501.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(437..1039)
                     /gene="fau"
                     /locus_tag="ACHK07_001140"
     CDS             complement(437..1039)
                     /gene="fau"
                     /locus_tag="ACHK07_001140"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO8101502.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(1060..1243)
                     /gene="ssrS"
                     /locus_tag="ACHK07_001141"
     ncRNA           complement(1060..1243)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK07_001141"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1285..1614)
                     /gene="zapA"
                     /locus_tag="ACHK07_001142"
     CDS             complement(1285..1614)
                     /gene="zapA"
                     /locus_tag="ACHK07_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8101503.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            1782..2360
                     /gene="ygfB"
                     /locus_tag="ACHK07_001143"
     CDS             1782..2360
                     /gene="ygfB"
                     /locus_tag="ACHK07_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO8101504.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            2386..3711
                     /gene="pepP"
                     /locus_tag="ACHK07_001144"
     CDS             2386..3711
                     /gene="pepP"
                     /locus_tag="ACHK07_001144"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO8101505.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            3708..4886
                     /gene="ubiH"
                     /locus_tag="ACHK07_001145"
                     /gene_synonym="visB"
     CDS             3708..4886
                     /gene="ubiH"
                     /locus_tag="ACHK07_001145"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO8101506.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            4909..6111
                     /gene="ubiI"
                     /locus_tag="ACHK07_001146"
                     /gene_synonym="visC"
     CDS             4909..6111
                     /gene="ubiI"
                     /locus_tag="ACHK07_001146"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO8101507.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(6185..6268)
                     /locus_tag="ACHK07_001147"
     CDS             complement(6185..6268)
                     /locus_tag="ACHK07_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101508.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            6559..7653
                     /gene="gcvT"
                     /locus_tag="ACHK07_001148"
     CDS             6559..7653
                     /gene="gcvT"
                     /locus_tag="ACHK07_001148"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO8101509.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVEKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            7677..8066
                     /gene="gcvH"
                     /locus_tag="ACHK07_001149"
     CDS             7677..8066
                     /gene="gcvH"
                     /locus_tag="ACHK07_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO8101510.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            8184..11057
                     /gene="gcvP"
                     /locus_tag="ACHK07_001150"
     CDS             8184..11057
                     /gene="gcvP"
                     /locus_tag="ACHK07_001150"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO8101511.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAGWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(11223..12656)
                     /gene="bglA"
                     /locus_tag="ACHK07_001151"
     CDS             complement(11223..12656)
                     /gene="bglA"
                     /locus_tag="ACHK07_001151"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO8101512.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            12701..13012
                     /gene="yqfB"
                     /locus_tag="ACHK07_001152"
     CDS             12701..13012
                     /gene="yqfB"
                     /locus_tag="ACHK07_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO8101513.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            13176..13835
                     /gene="yqfA"
                     /locus_tag="ACHK07_001153"
     CDS             13176..13835
                     /gene="yqfA"
                     /locus_tag="ACHK07_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO8101514.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(13912..14892)
                     /gene="ygfZ"
                     /locus_tag="ACHK07_001154"
     CDS             complement(13912..14892)
                     /gene="ygfZ"
                     /locus_tag="ACHK07_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO8101515.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            15135..15401
                     /gene="sdhE"
                     /locus_tag="ACHK07_001155"
     CDS             15135..15401
                     /gene="sdhE"
                     /locus_tag="ACHK07_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO8101516.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            15382..15789
                     /gene="ygfX"
                     /locus_tag="ACHK07_001156"
     CDS             15382..15789
                     /gene="ygfX"
                     /locus_tag="ACHK07_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO8101517.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(15829..16350)
                     /gene="fldB"
                     /locus_tag="ACHK07_001157"
     CDS             complement(15829..16350)
                     /gene="fldB"
                     /locus_tag="ACHK07_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO8101518.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            16462..17358
                     /gene="xerD"
                     /locus_tag="ACHK07_001158"
     CDS             16462..17358
                     /gene="xerD"
                     /locus_tag="ACHK07_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO8101519.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            17383..18093
                     /gene="dsbC"
                     /locus_tag="ACHK07_001159"
     CDS             17383..18093
                     /gene="dsbC"
                     /locus_tag="ACHK07_001159"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO8101520.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            18099..19832
                     /gene="recJ"
                     /locus_tag="ACHK07_001160"
     CDS             18099..19832
                     /gene="recJ"
                     /locus_tag="ACHK07_001160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO8101521.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            19923..21021
                     /gene="prfB"
                     /locus_tag="ACHK07_001161"
     CDS             join(19923..19997,19999..21021)
                     /gene="prfB"
                     /locus_tag="ACHK07_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO8101522.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            21031..22548
                     /gene="lysS"
                     /locus_tag="ACHK07_001162"
     CDS             21031..22548
                     /gene="lysS"
                     /locus_tag="ACHK07_001162"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8101523.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFGGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(22591..23139)
                     /gene="idi"
                     /locus_tag="ACHK07_001163"
     CDS             complement(22591..23139)
                     /gene="idi"
                     /locus_tag="ACHK07_001163"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO8101524.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNPVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            23194..23265
                     /locus_tag="ACHK07_001164"
     CDS             23194..23265
                     /locus_tag="ACHK07_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101525.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(23262..23387)
                     /gene="yqfG"
                     /locus_tag="ACHK07_001165"
     CDS             complement(23262..23387)
                     /gene="yqfG"
                     /locus_tag="ACHK07_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO8101526.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(23389..24837)
                     /gene="uacT"
                     /locus_tag="ACHK07_001166"
     CDS             complement(23389..24837)
                     /gene="uacT"
                     /locus_tag="ACHK07_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO8101527.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            25273..27192
                     /gene="ygfT"
                     /locus_tag="ACHK07_001167"
     CDS             25273..27192
                     /gene="ygfT"
                     /locus_tag="ACHK07_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO8101528.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHI
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGCDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            27192..27680
                     /gene="ygfS"
                     /locus_tag="ACHK07_001168"
     CDS             27192..27680
                     /gene="ygfS"
                     /locus_tag="ACHK07_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8101529.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLLGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     LL"
     gene            complement(27716..29083)
                     /gene="ghxQ"
                     /locus_tag="ACHK07_001169"
                     /gene_synonym="ygfQ"
     CDS             complement(27716..29083)
                     /gene="ghxQ"
                     /locus_tag="ACHK07_001169"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO8101530.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(29119..30435)
                     /gene="guaD"
                     /locus_tag="ACHK07_001170"
     CDS             complement(29119..30435)
                     /gene="guaD"
                     /locus_tag="ACHK07_001170"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO8101531.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(30453..31853)
                     /gene="xanQ"
                     /locus_tag="ACHK07_001171"
     CDS             complement(30453..31853)
                     /gene="xanQ"
                     /locus_tag="ACHK07_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO8101532.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(32018..34888)
                     /gene="xdhD"
                     /locus_tag="ACHK07_001172"
     CDS             complement(32018..34888)
                     /gene="xdhD"
                     /locus_tag="ACHK07_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO8101533.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(34885..35664)
                     /gene="ygfM"
                     /locus_tag="ACHK07_001173"
     CDS             complement(34885..35664)
                     /gene="ygfM"
                     /locus_tag="ACHK07_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO8101534.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(35715..37043)
                     /gene="ssnA"
                     /locus_tag="ACHK07_001174"
     CDS             complement(35715..37043)
                     /gene="ssnA"
                     /locus_tag="ACHK07_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO8101535.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLKAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(37046..40144)
                     /gene="ygfK"
                     /locus_tag="ACHK07_001175"
     CDS             complement(37046..40144)
                     /gene="ygfK"
                     /locus_tag="ACHK07_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO8101536.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(40466..41044)
                     /gene="mocA"
                     /locus_tag="ACHK07_001176"
     CDS             complement(40466..41044)
                     /gene="mocA"
                     /locus_tag="ACHK07_001176"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO8101537.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            41147..41917
                     /gene="yqeC"
                     /locus_tag="ACHK07_001177"
     CDS             41147..41917
                     /gene="yqeC"
                     /locus_tag="ACHK07_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO8101538.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            41965..43590
                     /gene="yqeB"
                     /locus_tag="ACHK07_001178"
     CDS             41965..43590
                     /gene="yqeB"
                     /locus_tag="ACHK07_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO8101539.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLTTSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(43811..44743)
                     /gene="arcC"
                     /locus_tag="ACHK07_001179"
     CDS             complement(43811..44743)
                     /gene="arcC"
                     /locus_tag="ACHK07_001179"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8101540.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(44791..46176)
                     /gene="hyuA"
                     /locus_tag="ACHK07_001180"
     CDS             complement(44791..46176)
                     /gene="hyuA"
                     /locus_tag="ACHK07_001180"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO8101541.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(46229..47440)
                     /gene="ygeY"
                     /locus_tag="ACHK07_001181"
     CDS             complement(46229..47440)
                     /gene="ygeY"
                     /locus_tag="ACHK07_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO8101542.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(47498..48694)
                     /gene="dpaL"
                     /locus_tag="ACHK07_001182"
     CDS             complement(47498..48694)
                     /gene="dpaL"
                     /locus_tag="ACHK07_001182"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO8101543.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVFSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(48752..49939)
                     /gene="ygeW"
                     /locus_tag="ACHK07_001183"
     CDS             complement(48752..49939)
                     /gene="ygeW"
                     /locus_tag="ACHK07_001183"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO8101544.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            50418..52196
                     /gene="ygeV"
                     /locus_tag="ACHK07_001184"
     CDS             50418..52196
                     /gene="ygeV"
                     /locus_tag="ACHK07_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO8101545.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGIVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(52236..52715)
                     /gene="xdhC"
                     /locus_tag="ACHK07_001185"
     CDS             complement(52236..52715)
                     /gene="xdhC"
                     /locus_tag="ACHK07_001185"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO8101546.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(52712..53590)
                     /gene="xdhB"
                     /locus_tag="ACHK07_001186"
     CDS             complement(52712..53590)
                     /gene="xdhB"
                     /locus_tag="ACHK07_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO8101547.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(53601..55898)
                     /gene="xdhA"
                     /locus_tag="ACHK07_001187"
     CDS             complement(53601..55898)
                     /gene="xdhA"
                     /locus_tag="ACHK07_001187"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO8101548.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVSRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            56314..57069
                     /gene="actS"
                     /locus_tag="ACHK07_001188"
     CDS             56314..57069
                     /gene="actS"
                     /locus_tag="ACHK07_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO8101549.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            57148..57221
                     /locus_tag="ACHK07_001189"
     tRNA            57148..57221
                     /locus_tag="ACHK07_001189"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57180..57182,aa:Gly,seq:ccc)
BASE COUNT        13885 a        14351 c        15092 g        14024 t
ORIGIN      
        1 aaagaatcaa cgatgtcaat cagggcgatg cgggatgtat cgccctgaac cgatcatttg
       61 atagacactt catcgcccac gtttactgcc gtaattaatc ccttatccct gcaatgtgtg
      121 tctgtcttac atgacgtttt acgagtgcgc tcgaagaaaa aatttaaagg tagatcaatg
      181 cgttgtgctt atttaaagag agtttacgat ggcagggcag catggggctg taacggtaaa
      241 gccctcaccg aagcgagggc ttgaaggaga agggttatga tgcgacttgt catcatactg
      301 attgtactgt tactcataag tttcagcgct tattaacagt cagtcacagg ggaggagcaa
      361 tcctccctta cccttactca ctaaattagg ttaaagaatc aacgatgtca atcagggcga
      421 tgcaggtgtg tcgccctcac cactcccaga ctttcgacgg tgtaaccacc gcaggaagag
      481 ggatatccca ctcttcaacg gggagttttt ccaccaactg acaatcatgc gcataaccca
      541 ccggttgcat tttatagtgc tgccagtttt gtaaggtccg atcataaaaa ccaccgccca
      601 ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg tgtgatcaac acgtctaatc
      661 gggaaagggg tagcacgtca cgcacatcca gttttggctc atggatcttc aacctgttca
      721 tcaccagttc actttgcgga tggtaattca ggaacagcaa attaccggca ctaaagggat
      781 gcaaaactgg aagatatacg cgcttaccgg cgcgccagag ttgttctata agcggctggg
      841 tgtcgagttc gccatcaaaa gagaggaata cagcgaccgt atgtgccatc accaccgggg
      901 gataagtcat catccgggta gcggcttgtt gacccatttc ctgctgttgt tccggcgtta
      961 acgtacgacg acgttgccga atcattttgc ggatttcttg tcgggataat gtcagtggga
     1021 gttctggtag ttgcgtcatg gctggtgcca gataagaagg gaatctccga gatgccgccg
     1081 caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt
     1141 ctcggacgga ccgagcatgc tcaccaaccg cggagcgcca cattcttgtg gtatgaaata
     1201 tcggctcagg ggactggccc gcttgcgaac atctcagaga aattttgtct tcacggttac
     1261 tctaccacag taaaccgaaa agtgtcattc aaagttttgg ttagtttttt cggtgatgcg
     1321 accttgttca agtaacgctt gttctatggt ctgctgcagc atccgaatac gctgttccat
     1381 acttgccgcg tagtcacgag tctttgcttt ttcttgcgct aactcatagc tgatattcaa
     1441 tgcggcaatg aagaccaact gttcagtatt tgtgactcta gtgcgttctt tcagatcttg
     1501 caaccgttgg ttcagatcgt ccgctgcctg attcaacgca tccctttggt caggcgggca
     1561 gttcacacgc agtgaacggc caaaaatttg gatatcgacg ggttgtgcag acatgccacc
     1621 ttcctgctga ttgactgcgc tgccttcgtc tttagaccca tggtctgcga aggggcgaca
     1681 ctatagctac cctgatgaga agagacaagc ccttttctgg tccaccaggg cccaaagtgg
     1741 tagcatatca tgaatattcc tccctttgac gacgaatgct tatgtctata cagaacgaaa
     1801 tgcctggtta caacgaaatg aaccagtatc tgaaccaaca agggacgggt ctgacccctg
     1861 ctgagatgca tggtttaatc agtgggatga tatgtggcgg taacgatgac agctcatggc
     1921 tgccgctact tcacgacctg acgaacgaag gcatggcttt cggtcatgag ctggcacagg
     1981 cactgcgtaa aatgcactct gccaccagcg atgccctgca ggatgacggc ttcctttttc
     2041 agctttatct gcctgatggc gatgatgtca gcgttttcga tcgggctgat gcgctggccg
     2101 gttgggtcaa tcacttcctg cttggtcttg gcgttacgca accgaagctg gacaaagtga
     2161 ccggcgaaac cggtgaagcc atcgacgatc tgcgtaacat cgcgcagttg ggctacgacg
     2221 aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg
     2281 ttgccgcgct gttatgccac gacaccttta ctcatccgca accgaccgcg ccagaagtac
     2341 aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc
     2401 cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc
     2461 gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg acagcgaata cccctatcgt
     2521 cagaacagtg acttctggta cttcaccggc tttaacgaac cggaagcggt gctggtgctg
     2581 attaaaagcg atgacactca taaccacagc gttctgttta accgcgttcg cgacctgacg
     2641 gcggagatct ggtttggccg tcgtttaggt caggatgccg cgccagagaa actgggcgtt
     2701 gaccgcgcac tggcattcag cgaaattaat cagcaacttt atcaactact taatggtctg
     2761 gatgtggttt accacgccca gggcgaatat gcatatgctg atgaaatcgt gaacagcgcg
     2821 cttgagaaac tgcgtaaagg ttcgcggcaa aatctcaccg cgccggcaac gatgatcgac
     2881 tggcgtccca ttgttcatga aatgcgcctg ttcaaatcgc cggaagagat tgccgtactt
     2941 cgccgcgcgg gtgaaatcac cgccctggcg catacccggg cgatggaaaa atgccgtccg
     3001 ggaatgttcg agtaccacct ggaaggcgaa attcaccacg aatttaaccg ccacggcgcg
     3061 cgctatcctt cctataacac cattgtcggc agcggtgaaa acggctgcat tctgcattac
     3121 accgaaaacg agtgtgaact gcgtgacgga gatctggtgt tgattgatgc gggttgtgaa
     3181 tacaaaggtt acgctggcga tattacccgc accttcccgg tcaacggcaa attcacccag
     3241 gctcagcgtg aaatctacga cattgtgctt gagtctctcg aaaccagcct gcgcctgtat
     3301 cgtccgggaa cttccattca ggaagtcacc ggtgaagtgg tgcgcatcat ggttagcggc
     3361 ctggtaaaac tcggcatcct gaaaggtgat gttgatgaac tgatcgctca gaacgcccat
     3421 cgtcctttct ttatgcatgg ccttagccac tggttaggac tggatgtcca tgacgttggc
     3481 gtttatggcc aggatcgctc gcgcattctg gaaccgggca tggtactgac cgtagagcca
     3541 gggctgtata ttgcgccgga tgcagaagtg ccagaacaat atcgcggtat cggcattcgt
     3601 attgaagacg acattgtgat taccgaaacc ggtaacgaaa acctcaccgc cagcgtggtg
     3661 aaaaagccgg aagaaatcga agcgttgatg gcagctgcga gaaagcaatg agcgtaatca
     3721 tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg
     3781 gggcgctgcc ggtacatttg attgaagcga ccgcgccaga gtcacatgct catccgggct
     3841 ttgatgggcg agcgattgcg ctggcggcgg gtacctgtca gcaactggcg cgcattggcg
     3901 tctggcaatc tctggcggat tgcgcaaccg ccatcactac cgtgcatgtc agcgatcgtg
     3961 gtcatgccgg attcgtcacc ctcgccgcag aagattacca actggcggcg ctggggcagg
     4021 ttgtcgaatt gcacaatgtc gggcaacggc tgtttgcatt gctgcgcaaa gcacctggcg
     4081 taacgctgca ttgcccggat cgcgtggcta acgttgcccg tactcagagt catgttgaag
     4141 tgacgctgga gtgtggcgag acgctgacgg gccgcgtgct ggtagccgct gatggcaccc
     4201 attcagcgtt agccactgtg tgcggcgttg actggcagca ggagccttac gaacaactgg
     4261 ctgtgattgc caacgttgcg acttccgttg cgcatgaagg gcgcgctttt gaacgcttta
     4321 cgcaacatgg cccgctggcg atgttgccga tgtctgacgg acgctgttcg ctggtctggt
     4381 gtcatccact ggaacggcgc gaagaggtgc tgtcgtggag cgacgagaag ttttgccgtg
     4441 aactccagtc ggcctttggc tggcgacttg ggaaaattac ccacgctggt aaacgcagtg
     4501 cttatccgct ggcgttaacc cacgccgcca gatctattac ccatcgtacc gtgctggtgg
     4561 gcaatgcggc gcaaaccctg cacccgattg ccgggcaagg gttcaacctc ggtatgcgag
     4621 atgtcatgag tcttgcggaa accctgactc aggcgcagga gcgcggagaa gacatgggtg
     4681 attacggcgt attgtgtcgt tatcagcagc gtcgacagag cgatcgcgaa gcaaccattg
     4741 gcgtcacgga cagccttgta catctttttg ccaaccgttg gacgccactg gttgtcgggc
     4801 gcaacatcgg gctgatgacg atggaattat tcaccccggc acgcgatgtg ctggcgcagc
     4861 gcaccctcgg ttgggtggcg cgttgaaggc ggttttaagg aataaacaat gcaaagtgtt
     4921 gatgtagcca ttgttggcgg cggcatggtg gggctggcgg ttgcctgcgg cttacagggg
     4981 agcggcttac gcgttgccgt actggagcag cgcgtaccgg aacctctggc ggtggatgca
     5041 ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg aaaaattact cacccgtctt
     5101 ggcgtctggc aagacattct ctctcgtagg gccagttgct atcacggtat ggaagtgtgg
     5161 gacaaagaca gttttggtca catttcgttt gacgatcaaa gcatgggcta tagccatctt
     5221 gggcatatcg ttgaaaactc agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg
     5281 tcagatatca ctctgttggc ccccgcagaa ttacagcagg tcgcctgggg agaaaacgaa
     5341 accttcctga cgctgaaaga tggcagtatg ttaacggcgc gtctggtgat tggcgcggac
     5401 ggcgctaatt cctggttgcg caacaaagcc gatattccgc tgactttctg ggattatcag
     5461 catcacgcgc tggtagcgac catacgcacg gaagaaccgc atgatgcggt ggcgcggcag
     5521 gttttccatg gcgaaggcat tctggccttt ttaccgctta gcgatccgca tctttgctcg
     5581 attgtctggt cactgtcgcc agaggaagcg cagcggatgc agcaggcaag tgaagacgaa
     5641 tttaatcgcg cgttaaatat cgcttttgat aatcgccttg gcttatgcaa ggttgagagc
     5701 gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc agtttgccgc gcatcgtctg
     5761 gcgctggtgg gcgacgccgc gcataccatt cacccgctgg cggggcaggg ggtaaatctc
     5821 ggctttatgg atgctgcaga gctgattgct gaactgaaac ggttgcatcg tcagggtaaa
     5881 gacatcgggc agtacattta cctgcgccgc tatgaacgta gccgcaagca cagcgcggcg
     5941 ctgatgctgg ctggtatgca gggttttcgc gatctctttt ccggcgctaa tccggcgaaa
     6001 aaactgctgc gtgatattgg tttgaaactg gccgacacgc ttcctggcgt taagccgcaa
     6061 cttatccgcc aggcaatggg attaaacgat ttgcctgaat ggctgcgtta aaaatttctc
     6121 ctctgttgtt tatttgatac ccatcacact ttcatctccc ggttttttcc ccgggagatt
     6181 ttcctcattt gaaataaact aatttcacct ccgttttcgc attatatttt ctaatgccat
     6241 gattttttga tttagtgttt tttgacattt ttttagctct taatattgtc ttattcaaat
     6301 tgactttctc atcacatcat ctttgtatag aaactggtgt gttttttggt tttttattct
     6361 gtcgcgattt ttgcattttt taaccataag ctaatgtgat gatcaatttt accttatggt
     6421 taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg gggaccggga
     6481 gtggctccga tgctgggttt tgtggtgata atttcaccat gaaaaagttg tcagccccgc
     6541 ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca cacgctttgc
     6601 ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc
     6661 gacgaacatc atgcggtacg taccgatgcc ggaatgtttg atgtgtcgca tatgaccatc
     6721 gtcgatctcc gcggcagccg cacccgggag tttctgcgtt atctgctggc gaacgatgtg
     6781 gagaagctca ccaaaagcgg caaagccctt tactcgggga tgttgaatgc ctctggcggt
     6841 gtgatagatg atctcatcgt ctactacttt actgaagatt tcttccgcct cgttgttaac
     6901 tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc tttcggcatc
     6961 gaaattaccg ttcgcgatga cctttccatg attgccgtgc aagggccgaa tgcgcaggca
     7021 aaagctgcca cactgtttaa tgacgcccag cgtcaggcgg tggaagggat gaaaccgttc
     7081 tttggcgtgc aggcgggcga tctgtttatt gccaccactg gttataccgg tgaagcgggc
     7141 tacgaaattg cgctgcccaa tgaaaaagcg gcggatttct ggcgcgcgct ggtggaagcg
     7201 ggcgttaagc catgcggcct gggcgcgcgt gacacgctgc gtctggaagc gggtatgaat
     7261 ctttatagtc aggagatgga cgaaaccatc tctcctttag ccgccaatat gggctggact
     7321 atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg aagccctgga agtgcagcgt
     7381 gagcatggta cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat
     7441 gaactgccgg tacgttttac cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc
     7501 gggactttct ccccgacgct gggttacagc attgcgctgg cgcgcgtgcc ggaaggtatt
     7561 ggtgaaacag cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt gacgaaacct
     7621 gtttttgtgc gtaacggcaa agccgtcgcg tgatttactt ttttggagat tgattgatga
     7681 gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt aaagaagccg
     7741 acggcactta caccgttggc atcaccgaac acgctcagga gctgttaggc gatatggtgt
     7801 ttgttgacct gccggaagtg ggcgcgacgg ttagcgcggg cgatgactgc gcggttgccg
     7861 aatcggtaaa agcggcgtca gacatttatg caccagtaag cggtgaaatc gtggcggtaa
     7921 acgacgtact gagcgattcc ccggaactgg tgaacagcga accgtatgca ggcggctgga
     7981 ttttcaaaat caaagccagc gatgaaagcg aactggaatc actgctggat gcgaccgcat
     8041 acgaagcatt gttagaagac gagtaacggc tttattcctc ttctgcggga gaggatcagg
     8101 gtgaggaaat ttacgcctca ccctcactct cttcgtaagg agagaggttc acaattcact
     8161 gcacgtttca ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aatagcggcg
     8221 cttttattga acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg
     8281 ttggcgcaca atcgttaaac gcgctgaccg gccagattgt accgaaagat attcagcttg
     8341 cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc tgcactggca gaactcaagg
     8401 ctattgccag tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc
     8461 agttaccgcc ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtata
     8521 ctccgtatca acctgaagtc tcgcagggcc gccttgaggc actgctcaat ttccagcagg
     8581 taacgctgga tttaaccggg ctggatatgg cctctgcttc tcttcttgac gaagccaccg
     8641 ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct
     8701 tctttgttgc ttctgacgtt catccgcaaa cgctggatgt ggtacgtact cgtgctgaaa
     8761 cctttggttt tgaagtgatt gtcgatgatg cgcaaaaagt gctcgaccat caggacgtct
     8821 tcggcgtgct gttacagcag gtcggtacta ctggtgaaat tcacgactac actgcgctta
     8881 tcagcgaact gaaatcacgc aaaattgtgg tcagcgtcgc cgccgatatt atggcgctgg
     8941 tgctgttaac tgcgccgggt aaacagggcg cagatattgt ctttggttcg gcgcaacgct
     9001 tcggcgtgcc gatgggctac ggtggcccac acgcggcatt ctttgcggcg aaagatgaat
     9061 acaaacgctc aatgccaggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg
     9121 cgctgcgcat ggcgatgcag actcgcgagc aacatattcg tcgtgagaaa gcgaactcca
     9181 acatttgtac ttcccaggtg ctgctggcaa acatcgccag cctgtatgcc gtttatcacg
     9241 gcccgattgg cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg
     9301 caggcctgca acaaaaaggt ctgaagctgc gccatgcgca ctatttcgac actctgtgtg
     9361 tggaagtggc cgacaaggcg ggcgtactgg cgcgtgccga agcggctgaa atcaacctgc
     9421 gtagcgatat cctgaacgcg gttgggatca cccttgatga aaccaccacg cgcgaaaacg
     9481 tgatgcagct tttcagcgta ctactggggg ataatcacgg gctggacatc gacacgctgg
     9541 acaaagacgt ggctcacgac agccgctcta ttcaacctgc gatgctgcgt gatgacgaaa
     9601 tcctcaccca tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc
     9661 actcgctgga gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca
     9721 ccatgaaact gaacgccgcc gccgagatga tccccatcac ctggccggaa tttgccgaac
     9781 tgcacccttt ctgcccgccg gaacaggccg aaggttatca gcagatgatc gcgcagctgg
     9841 ctgactggct ggtgaaactg accggttacg acgccgtctg tatgcagccg aactctggcg
     9901 cacagggcga atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag
     9961 ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg cactaaccct gcttctgcac
    10021 atatggcagg aatgcaggtg gtggttgtgg catgtgataa aaacggcaac atcgatctgg
    10081 ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct ctcctgtatc atggtgacct
    10141 atccttccac ccacggtgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc
    10201 agttcggcgg tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct
    10261 cgccaggctt cattggcgcg gacgtttcgc acctcaacct gcataaaact ttctgcattc
    10321 cgcacggcgg tggtggtccg ggtatgggac cgatcggtgt gaaagcgcat ctggctccgt
    10381 ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcggttt
    10441 ctgcggcacc gttcggtagc gcctctatcc tgccaatcag ctggatgtac atccgcatga
    10501 tgggcgcaga agggctgaaa aaagcaagtc aggtggcaat cctcaacgcc aactatattg
    10561 ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg tcgtgacggt cgcgtggcgc
    10621 acgaatgtat tctcgatatt cgtccgctga aagaagaaac cggcatcagc gagctggata
    10681 ttgccaagcg cctgatcgac tacggtttcc acgcgccgac catgtcgttc ccggtggcgg
    10741 gtacgctgat ggttgaacca actgaatctg aaagcaaagt ggaactggat cgctttatcg
    10801 atgcgatgct ggctatccgc gcagaaattg accaggtgaa agccggtgtc tggccgctgg
    10861 aagataaccc gttggtgaac gcgccgcaca ttcagagcga actggtcgcc gggtgggcgc
    10921 atccgtacag ccgtgaagtt gcggtattcc cggctggcgt ggcagacaaa tactggccga
    10981 cggtgaaacg tctggatgat gtttacggcg accgtaacct gttctgttcc tgcgtaccga
    11041 ttagcgaata ccagtaattc actgattcga ctctcttcta aaggcgcttc ggcgcctttt
    11101 tagcttgtta ataaagtgcg ctttgtgtgt ttcggctgag gcgtaatata aaatcttgcg
    11161 agctcaatat attaaaagag tgccaggcct gatgagcgta tcgcatcagg cacatcggct
    11221 aattacagat tctcgccgtt gctggcaatc acctctttgt accagttaaa gctcttctta
    11281 cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag
    11341 cgtttgctgt actgcccggt ggtgaacgac acgcagtcga tgcagcccca cggtgtgtag
    11401 cccatcagat ccacgccatc gtaagtcacc gcttttttca tctcttcgat atgggcgcgc
    11461 aggtagtcaa tgcggtagtc atcgttgatg ctgccatctt cttccacttt gtcgtaagcg
    11521 ccaaaaccgt tttcgacaat aaacagcggc ctctgataac gctcatacag ttcgcaaagg
    11581 gcatagcgca gacctactgg atcaatctgc cagccccagt cagacgcttt aacatacggg
    11641 ttcggtacgc tgccttcgaa accagagatc gcatcgccgg tgccgccttc ggccttcact
    11701 gcgttggtca tgtaatagct gaaaccaaga taatcgcagg tgccttcgcg cagtatatcg
    11761 agatcgccat cttccatttt gatgttaaat ccgcgacgct cccactcgtt caacacatag
    11821 gacgggtaat agccgcgtag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc
    11881 tgagcgaaca tcacatcgtc cgggttacag gagtaaggat agagcggcac catcgccagc
    11941 atacagccga ctttcatctc cgggttaatg cgacgcgcag ctttcaccgc cagggcgctg
    12001 gcgacaaact ggtgatgcag cacctgatac atcgtctctt ccgggttctc atgctcggta
    12061 taaaccacgc cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta
    12121 atttcgttga aggtcatcca gtatttgact ttatgcttat agcgttcaaa taccacttcc
    12181 gcgaaacgga caaagaaatc aaccacttta cggttggtcc agctaccgta ttgctgcacc
    12241 agatgcagcg gcatttcaaa gtgggagagg gtgatcactg gttcgatgtt gtatttcagc
    12301 aattcatcga acagagagtc gtaaaacttc agcccttctt cgtttggctg agcttcatcg
    12361 ccttttggaa aaatgcgcgt ccaggcgatg gatgtacgaa aacatttgaa gcccatttcg
    12421 gcaaatagct tgatgtcttc cttgtagtga ccataaaaat caacggcttc atggtttgga
    12481 tagtattttc ctggcaagac ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg
    12541 gtcagaacgt cacaaatgct cggccctttg ccgcctttgt tccagccgcc ttcgacctga
    12601 tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc
    12661 ataggctcca gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac
    12721 tttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga
    12781 cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcgggcgtt ttgaagatga
    12841 cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaacgctgg atacgctgac
    12901 ggaaaaacat gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga
    12961 catctatccc ggtcagacac agttttatgt gattgaattt aaatgtcttt aaattgaact
    13021 cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt tttaggagat tagtctcaaa
    13081 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
    13141 tatacatgtg cgtgtacgtt tctccggagt aagttatggt tcagaagccc ctcattaagc
    13201 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggttggtgt
    13261 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
    13321 cagcgataac cagctacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
    13381 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
    13441 gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
    13501 ctccgttagc gcgcgggttg atgattgtta tctggagcct ggcattgctg gggattctgt
    13561 tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacttat ctggcgatgg
    13621 gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta
    13681 ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac gtctgcaaac
    13741 gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt gtgtgccact
    13801 ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt aggcctgata
    13861 agacgcgcca gcgtcgcatc aggcgataaa tgcctgatgc gacacagaga tttactcttc
    13921 gagcgaatac ggcagcggct cgatatgcaa tgtattcgca tcgtcacgta cgcggaagat
    13981 gctgtccggt tccatatcgt tattcatcac tacctgtacc acgacctgac catcttccag
    14041 ttttaccgca gccagcactg taccggtacg acgccagttc tcgcccattt tcagctctaa
    14101 gtcttcacca gcttccggca gtcggctggc actacctgtc agcaaccaga gcgcacgttt
    14161 attggcacca cggaattttg ctcgcgccac catctcttgt ccggtataac agcctttctt
    14221 aaagctgata ccgcccagcg cctggagatt ggtcgcctgt gggataaact gcccgctgtt
    14281 cgcggcatca atcaccggga aacccgcttc aatgtttaat gccagccact gttggctatt
    14341 gttcagttcc gcttcaccgc gcagtttatc ggtcagcata ttggcagtag cttcatcggt
    14401 tacgatcagg aaacgttctg ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt
    14461 aactacctgt ttttctctcg aaggtagttc actaaagaga ttggccagcg cggcgcgcgc
    14521 ctgaaaaccg gcaacaccaa gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga
    14581 gaacaccgca tattttttca gttcagtcag ctgcggttca cgcacgctgc gccgttcaat
    14641 ccacgcaaag ccatcgccgt cgcggaacag acgtaaattg ctccacattt tacctttggc
    14701 gtcgcaatgg gcggcgagca ggtgctgatt ttctgtcatc tggctgacat ctgctgtcac
    14761 ctggccctgc atatattttt cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc
    14821 aagcgtcatc agcgtcagcg gtaaacgggc agaagccgta ggctgacgtg gaggaaaagg
    14881 tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt attaataact aatggtaaaa
    14941 gagctatgag gcaatgcaag tgctttcact acgcgatctg tgccttctgc gaggcgactt
    15001 ccaaaaaaat gaagcccttg aattgttgtg aattaatttc gcgatcgcga ttgtgtttac
    15061 tgatattccc gtaaaagaag tcgaaaaaca cgttacaata gcggggtact ggattttcgt
    15121 gagacaggaa gaagatggac attaacaaca aagcccgcat tcattgggca tgccgccgtg
    15181 gtatgcgcga actcgatatt tcaatcatgc cgtttttcga acatgagtac gacagcttaa
    15241 gcgatgacga aaaacgcatc tttattcgtc tgctggaatg tgacgatcca gacctgttta
    15301 actggctgat gaatcacggt aaaccggccg atgcagaact ggaaatgatg gtccgactca
    15361 tccagacacg gaaccgggaa cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc
    15421 gcacagtggc tttccttgct gattcatggg ctggttgccg ctgttatttt actcatgccc
    15481 tggccgctca gttacacccc gttatggatg gtgttacttt cgctggtggt gtttgattgc
    15541 gttcgcagcc agcggcgtat taatgctcgc cagggggaaa ttcgcttgtt gatggacggg
    15601 cgtttgcgtt ggcaagggca ggagtggagc atcgtcaaag caccgtggat gattaagagc
    15661 ggcatgatgc tgcgtttacg ttctgatggc ggtaaacggc aacatttatg gctggcagcc
    15721 gacagcatgg acgaagccga atggcgggat ttacggcgga ttttgttgca acaagagacg
    15781 caaagataaa gatgacggca aagcatatgc gctaagccgt cagtgacatc aggcgtaatg
    15841 ctctgccatt tcgttgagga tttgttcgca ccagctctga atacgctcgt cactaaggtc
    15901 atactggtta gtttcgtcca gcgccagccc cacgaacagt tgcccatcag caatcaccgg
    15961 tttcgggctg gtaaattcat atccttccgt tggccagtag ccgacgaact tcacgccttt
    16021 ggtcgagagt ttgtcatgca gcataccgag cgcatcgagg aaccactcgc cgtatcccag
    16081 ttgatcgcca agcccataca gcgcaacaat tttaccttcg aggttcaggt cgtcgagctg
    16141 atcccagacg gcttcccagt cttcctggat ttcaccaaaa tcccaggtcg ggatacccag
    16201 aatgagcaca tcgtactgct ccattaattt cggggagtcg tccttgaggt tatgtaaggt
    16261 caccagttct gggccgataa tatcgcggat tttttctgcc gccatttcgg tgtaacaggt
    16321 gctggaaccg taaaaaagac ccatattcat aacgtaagct tctcgattct gctgtggctt
    16381 ttgcggctag tgtatcagaa tcgctttagt cacggcacaa taaggcataa tggatcaaat
    16441 gagtgaccat aaggggcgca agtgaaacag gatctggcac gcatcgagca gtttcttgat
    16501 gctctgtggc tggaaaaaaa tctggctgaa aatacgttga acgcttaccg tcgcgatctg
    16561 tcaatgatgg tggagtggtt gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac
    16621 gatttgcagg cattactggc agaacggctg gagggcgggt ataaagccac cagctcagcg
    16681 cgtttgctga gtgcagtgcg ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa
    16741 gacgatccca gtgcgcatct cgcttcaccg aaattgcccc agcgtttgcc aaaagattta
    16801 agcgaagcgc aggttgaacg tttattacag gcaccattaa ttgatcagcc actggagcta
    16861 cgcgataaag ccatgcttga agtgttgtat gctaccgggc tgcgtgtctc tgaactggtc
    16921 ggactgacaa tgagtgatat cagcctgcgt cagggcgtag tgcgggtcat tggtaaaggc
    16981 aacaaagagc gcctggtacc gttaggtgaa gaggcggttt actggctgga aacctatctg
    17041 gaacatgggc gtccgtggct gctgaatggt gtgtcaattg acgtgttgtt tcccagccag
    17101 cgtgcgcagc agatgacgcg acagaccttc tggcaccgta ttaaacatta tgccgtgctg
    17161 gcgggtatcg acagcgaaaa gctgtcaccg catgtgttgc gccacgcttt tgccactcat
    17221 ttattaaatc atggtgcgga tttacgcgtg gtgcagatgc tactgggcca cagcgatctt
    17281 tccaccacgc aaatttatac gcatgtcgct accgagcgtc tgcggcaact tcatcaacag
    17341 catcacccgc gggcgtgatg tctgaaaaga acgggaagat ttatgaagaa aggttttatg
    17401 ttgtttactt tgttagcggc gttttcaggc tttgctcagg ctgatgacgc ggcaattcaa
    17461 caaacgttag ccaaaatggg catcaaaagc agcgatattc agcccgcgcc cgtagctggc
    17521 atgaagacag ttctgactaa cagcggtgtg ttgtacatca ccgatgatgg taaacatatc
    17581 attcaggggc caatgtatga cgttagcggc acggctccgg tcaatgtcac caataagatg
    17641 ctgttaaagc agttgaatgc gcttgaaaaa gagatgatcg tttataaagc gccgcaggaa
    17701 aaacacgtca tcaccgtgtt tactgatatt acctgtggtt actgccacaa actgcatgag
    17761 caaatggcag actacaacgc gctggggatc accgtgcgtt atcttgcttt cccgcgccag
    17821 gggctggata gcgatgctga gaaagaaatg aaagctatct ggtgtgcgaa agacaaaaac
    17881 aaagcgtttg atgatgtgat ggcaggtaaa agcgtcgcac cagccagttg cgacgtggat
    17941 attgccgacc attacgcact tggcgtccag cttggcgtta gtggtactcc ggcagttgtg
    18001 ctgagcaatg gcacacttgt tccgggttac cagccgccga aagacatgaa agaattcctc
    18061 gacgaacacc aaaaaatgac cagcggtaaa taattcgcgt gaaacaacag atacaacttc
    18121 gtcgccgtga agtcgatgaa acggcagact tgcccgctga attgcctccc ttgctgcgcc
    18181 gtttatacgc cagccgggga gtacgcagtg cgcaagaact ggaacgcagt gttaaaggta
    18241 tgctgccctg gcagcaactg agcggcgtcg aaaaggccgt tgagatcctt tacaacgctt
    18301 ttcgcgaagg aacgcggatt attgtggtcg gtgatttcga cgctgacggt gccaccagca
    18361 cggctctaag cgtgctggcg atgcgctcgc ttggttgcag caatatcgac tacctggtac
    18421 caaaccgttt cgaagacggt tacggcttaa gcccggaagt ggtcgatcag gcccatgccc
    18481 gtggcgcgca gttaattgtc acggtggata acggtatttc ctcccatgcg ggggttgagc
    18541 acgctcgctc gttgggcatc ccggttattg ttaccgatca ccatttgcca ggcgacacat
    18601 tacccgcagc ggaagcgatc attaacccta acttgcgcga ctgtaatttc ccgtcgaaat
    18661 cactggcagg cgtgggtgtg gcgttttatc tgatgctggc gctgcgtacc tttttgcgcg
    18721 atcagggctg gtttgatgag cgtaacatcg caattcctaa cctggcagaa ctgctggatc
    18781 tggtcgcgct ggggacagta gcggacgtcg tgccgctaga cgctaataat cgcattctga
    18841 cctggcaggg gatgagtcgc atccgtgccg gaaagtgccg tccggggatt aaagcgctgc
    18901 tggaagtggc aaaccgtgat gcacaaaaac tcgccgccag cgatttaggt tttgcgctgg
    18961 ggccacgtct caatgctgcc gggcgactgg acgatatgtc cgtcggtgtg gcgctgttgt
    19021 tgtgcgacaa catcggcgaa gcgcgcgtgc tggcaaatga actcgatgcg ctaaaccaga
    19081 cgcgaaaaga gatcgaacaa ggaatgcaaa ttgaagccct gaccctgtgc gagaaactgg
    19141 agcgcagccg tgacacgcta cccggcgggc tggcaatgta tcaccccgaa tggcatcagg
    19201 gcgttgtcgg tattctggct tcgcgcatca aagagcgttt tcaccgtccg gttatcgcct
    19261 ttgcgccagc aggtgacggt acgctgaaag gttccggtcg ctccattcag gggctacata
    19321 tgcgtgatgc gctggagcga ttagacacac tctaccccgg tatgatgctc aagtttggcg
    19381 gtcatgcgat ggcggcgggt ttgtcgctgg aagaggataa attcgaactc tttcaacaac
    19441 ggtttggcga actggttact gagtggctgg acccttcgct attgcaaggc gaagtggtgt
    19501 cagacggtcc gttaagcccg gccgaaatga ccatggaagt ggcgcagctg ctgcgcgatg
    19561 ctggcccgtg ggggcagatg ttcccggagc cgctgtttga cggtcatttc cgtctgctgc
    19621 aacagcggct ggtgggcgaa cgtcatttga aggtgatggt cgaaccggtc ggcggcggtc
    19681 cactgctgga tggtattgct tttaatgtcg ataccgccct ctggccggat aacggcgtgc
    19741 gtgaagtgca actggcttat aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc
    19801 aaattatcat cgacaatatc tggccaattt agcgtcatct tctctataaa aaagagcgtg
    19861 gattgggtac aatcccgctc ttatcaccgc attttgacta gctcaataaa agaaatcaga
    19921 ccatgtttga aattaatccg gtaaataatc gcattcagga cctcacggaa cgctccgacg
    19981 ttcttagggg gtatctttga ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc
    20041 gagctggaac agccggatgt ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag
    20101 cgttcctccc tcgaagccgt tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat
    20161 gtttctggtc tgctggaact ggctgtagaa gctgacgacg aagaaacctt taacgaagcc
    20221 gttgctgaac tcgacgccct ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc
    20281 tctggcgaat atgacagcgc cgactgctac ctcgatattc aggcggggtc tggcggtaca
    20341 gaagcacagg actgggcgag catgcttgag cgtatgtatc tgcgctgggc agaatcgcgt
    20401 ggtttcaaaa ctgaaatcat cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg
    20461 actatcaaaa tctccggcga ttacgcttac ggctggctgc gtacagaaac cggcgttcac
    20521 cgcctggtgc gtaaaagccc gtttgactcc ggcggtcgtc gccatacgtc gttcagctcc
    20581 gcgtttgttt acccggaagt cgatgacgat attgatatcg aaatcaaccc ggcggatctg
    20641 cgcattgacg tttatcgcgc gtccggcgcg ggcggtcagc acgttaaccg taccgaatct
    20701 gcggtgcgta ttactcacat cccaaccggg atcgtgaccc agtgccagaa cgaccgttcc
    20761 cagcacaaga acaaagacca ggccatgaag cagatgaaag cgaagcttta tgaactggag
    20821 atgcagaaga aaaatgccga gaaacaggcg atggaagata acaaatccga tatcggctgg
    20881 ggcagccaga ttcgttctta tgtccttgat gactcccgca ttaaagatct gcgcactggg
    20941 gtagaaaccc gcaacacgca ggccgtgctg gacggcagcc tggatcaatt tatcgaagca
    21001 agtttgaaag cagggttatg aggaaccaac atgtctgaac aacacgcaca gggcgctgac
    21061 gcggtagtcg atcttaacaa tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgc
    21121 gagcagggga ttgccttccc gaacgatttc cgtcgcgatc atacctctga ccaattgcac
    21181 gcagaattcg gcggtaaaga gaacgaagaa ctggaagcgc tgaatatcga agtcgccgtt
    21241 gctggccgca tgatgacccg ccgtattatg ggtaaagcgt ctttcgttac cctgcaggat
    21301 gtgggtggcc gcattcagct gtacgttgcc cgtgacgatc tgccggaagg cgtttacaac
    21361 gagcagttca aaaaatggga cctcggcgac atcctcggcg cgaaaggtaa actgttcaaa
    21421 accaaaaccg gcgaactgtc tatccactgt accgagctgc gtctgctgac caaagcactg
    21481 cgtccgctgc cggataaatt ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt
    21541 tatctggatc tcatctctaa cgatgaatcc cgtaacacct ttaaagtgcg ctcgcagatc
    21601 ctctctggta ttcgccagtt catggtgaac cgcggcttta tggaagttga aacgccgatg
    21661 atgcaggtga tcccaggcgg tgccgctgcg cgtccgttta tcacccacca taacgcgctg
    21721 gatctcgaca tgtacctgcg tatcgcgccg gaactgtacc tcaagcgtct ggtggtcggt
    21781 ggcttcgagc gtgtattcga aatcaaccgt aacttccgta acgaaggtat ctccgtacgt
    21841 cataacccag agttcaccat gatggaactc tacatggctt acgcagatta caaagatctg
    21901 atcgagctga ccgaatcgct gttccgtact ctggcacagg atattctcgg taagacggaa
    21961 gtgacctacg gcgacgtgac gctggacttc ggtaaaccgt tcgaaaaact gaccatgcgt
    22021 gaagcgatca agaaatatcg cccggaaacc gacatggcgg atctggacaa cttcgactct
    22081 gcgaaagcga ttgctgaatc tatcggtatc cacgttgaga agagctgggg tctgggccgt
    22141 atcgttaccg agatcttcga agaagtggca gaagcacatc tgattcagcc gaccttcatt
    22201 actgaatatc cggcagaagt ttctccgttg gcgcgtcgta acgacgttaa cccggaaatc
    22261 acagaccgct ttgagttctt cattggtggt cgtgaaatcg gtaacggctt tagcgaactg
    22321 aatgacgcgg aagatcaggc gcagcgcttc ctggatcagg ttgccgcgaa agatgcaggt
    22381 gacgacgaag cgatgttcta cgatgaagat tacgtcaccg cactggaaca tggcttaccg
    22441 ccgacagcag gtctggggat tggtatcgac cgtatggtaa tgctgttcac caacagccat
    22501 accatccgcg acgttattct gttcccggcg atgcgtccgg tgaaataagc attacgttat
    22561 gctcacaacc ccggcaaatg tcggggtttt ttatttaagc tgggtaaatg cagataatcg
    22621 tattctggct tcgcgatttg tcgcctgcat caccatccac ggactgaacg cccacggcgt
    22681 ggcatcaata ccgcgtaata catcagctaa atcacaccat tgataatcca tcacttcatc
    22741 atcgttgatc tgtaacgcac tggtggtgcg tgcggcaaat accggacaca cttcattttc
    22801 cacaatgcca ttcggatcgg tggcgcgata gcgaaagtca ggatagatag attcaggagg
    22861 cgtaatttcc acgccaagct cataacggca acggcggatc accgcgtctt cgttgctttc
    22921 tcccagttgt gggtgcccac aaaccgggtt agtccacacg ccaggccatg cttttttgct
    22981 cagtgcgcgg cgggtaacta ataattgtcc tttggcatta aacagccaac tggagaacgc
    23041 gagatgtaag cgggtgtctg ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac
    23101 tccctgtgca ttcaataaaa tgacgtgttc cgtttgcata atttctcaca tgtaattctg
    23161 atcactcatc attatagata attgaagagt gaaatgatta accaagtgag cgtttatcga
    23221 caaccgcccg ttttgagcgg atgtcgacag gtaaaaacca tttaaaaaag tagcatcata
    23281 tatttgaagc gactttcaat ggccattgca acacagtcag aaatgacagg aatagagaga
    23341 gtaaaaagca acagcagact gaatatagcg cgcattaaaa aattcatatt attctccatg
    23401 ctcatttttc ttcagcttgc gcatcagaag ccacatacgt actgtcctga cttttaaatc
    23461 tttatcagag acggattcct tcaccaggtc agcatgatgc tggtagccat taaagaagac
    23521 attcagcaca acggcgctaa gggttgcgag cataatgccg ctatgcagca gcggttgcag
    23581 tacggccggt aacttagaaa agaaatcgtg agagagcgtc ggagtcatgc caacgccgag
    23641 actgatcgcc acaatataga ggttgtaacg gttggtggtg tagttacagc gcgacagaat
    23701 tcgaatccct gtcgccagta ccatgccgaa catcaccagc ccagcgccgc ccagcacaaa
    23761 ttgcggaatg gaggctacca gcaccgccat ttttggcacc atgccaaaca ggattaatat
    23821 aattcccgaa gaaatacaca cccagcggct atgaacgcgc gtcacgctaa ccaggccaac
    23881 gttttgagaa aatgacgtat gggggaagct gttaaacgtg ccgcctatca ttgtccctac
    23941 gccatcgaca cgcagcccgc gaataatatc gtgtgaagag agtttacgac caactatttc
    24001 acccagtgcc aggaacatcc ccatcgactc gataaacacg atgattaaca cggcagtcat
    24061 ggtcagaatg gaaacgggat caaaaatcgg cataccaaac gacatcggcg taacaatcgc
    24121 aaaccatgaa gcatcatgta gcccggataa attgacttca ttcatcatcc acgaaagtaa
    24181 aaagccaaat acaatcccca gtaatacggc gacgttggac ataaatcctt tcgcatagcg
    24241 agtaatgagc aagataaaaa ttaagacggc aaaggagata cctaaataaa cgggattacc
    24301 atattgcgga ttccctttac ctccggcggc ccagtcaata cccacctgaa tgatgctaag
    24361 cccgatagaa gtaataacca caccggtaac cagtggcgga aataaaggca tcaagcgacc
    24421 gataagtggc gctaataatg tggtgataaa acccgcggcg atagtggcac caaatatccc
    24481 cagcaggccg atatccgggt tcatcccaat ggctatcatt ggtgttacag cagcaaaggt
    24541 caccgacata atcaccggca ggcggatccc cataaagcgg ccgataccga tacattgcaa
    24601 taatgtgacg atcccgcagc aaaagagatc cgagctaatg agcatcgcaa tagcatcttt
    24661 tgagaggccc agtcggtcac caatcataag aggaacagcg actgcacctg cgtacatgac
    24721 aaggacgtgt tgcagaccga gtatgatcag ctttcctggt gataatatgc ggtcaacctc
    24781 atcagttggg cggtcttgcc cagaagatga gggaagttgg gaatctatgg cgctcatcag
    24841 tgtttctcct ttcgcccgcc agagtaaaaa gcaaccgttt acgttaatga aactgtctaa
    24901 acacatgctc gtggagtgcc agtttttgcg aggaagggga gttagagaca ggaattgcgg
    24961 aggcaatcac ataatcgtgc ttaaaccacg aaaatgagaa gagaacctga caatggaatt
    25021 atctgtaatt ttctcatatt gatatttcat taatcattct ggcatgtcgc actctcgcat
    25081 ttaatcgttt ttatctggat agcgctcttt tgatcggcaa tgttgaaata atgaaagcgg
    25141 catagattga ggggcaataa tttgaactca gtcgcaaaat tataattaaa acggtcatgc
    25201 tacctaatat atgcccgttt attgcatcgc gaaatttgag aagacataac tcattaaatg
    25261 aaagggatgc aaatgaataa gtttatcgct gctgaagctg cggaatgtat aggctgtcat
    25321 gcttgtgaaa ttgcctgtgc ggtggcacac aatcaagaaa actggccgct gagtcacagt
    25381 gactttcgac cacgtatcca cattgtaggg aaaggccagg ctgcgaatcc ggtggcctgc
    25441 catcactgca acaatgcccc ttgcgttacg gcttgtccgg ttaatgctct gactttccag
    25501 tccgatagcg tacaactgga cgagcaaaaa tgtattggtt gtaaaagatg cgcaatcgct
    25561 tgcccctttg gcgtcgttga gatggtcgat acgattgcgc agaaatgcga cctttgtaac
    25621 cagcgcagtt ccggcacgca agcctgtatt gaagtttgcc caacacaggc gttacgactg
    25681 atggacgata aagggttaca gcagataaag gtggcccgcc agcgcaaaac ggcagcagga
    25741 aaagcgtcat cagacgctca gccatctcgc agtgcagcgt tgctccccgt taactcgcgt
    25801 aaaggcgcag ataaaatttc agcaagtgaa cggaaaaacc attttggcga aatctattgc
    25861 gggctggatc cacaacaagc gacttatgag agtgaccgct gtgtttattg tgccgaaaaa
    25921 gctaactgca actggcattg cccgctgcat aacgctattc cggattacat ccgtctggta
    25981 caggagggaa agattattga agcggcagaa ctttgccacc agaccagttc cttaccagaa
    26041 atctgcggca gggtatgtcc acaagaccgt ctttgtgaag gcgcatgtac tttgaaagat
    26101 cactctggcg cagtctctat cggtaatctg gaacgctaca tcaccgatac cgcgctggcg
    26161 atgggctggc gtcccgatgt cagcaaagtt gttccccgta tcgagaaagt ggcggtgatt
    26221 ggcgctgggc ctgcagggtt agggtgtgct gatattctgg cgcgcgcagg agttcaggtc
    26281 gatgtctttg atcgccatcc agaaattggc ggtatgctga cttttggcat tcctcctttc
    26341 aaactcgata aaacggtatt aagccagcgg cgagagatat tcaccgcaat gggaatcgac
    26401 tttcatctta actgtgaaat tggctgcgat attaccttta gcgatttaac ttctgaatat
    26461 gatgcagttt tcatcggcgt ggggacttac gggatgatgc gagctgatct gccgcatgaa
    26521 gatgcgcccg gtgtcattca ggctctaccg ttcttgactg cccatacccg ccagctcatg
    26581 gggttgccgg agtctgaaga gtatccgctg acggacgtgg aaggtaagcg agtcgtggta
    26641 ttgggcggtg gcgatacgac aatggattgt ttgcggactt ccatccgcct caatgccgcc
    26701 agcgtgacct gcgcgtatcg tcgtgatgaa gtcagtatgc cgggctcgcg caaagaggtg
    26761 gtcaatgcgc gcgaggaagg tgtcgagttt caattcaatg ttcagccgca atatatcgct
    26821 tgtgacgaag atgggcgttt aactgcggtg ggcctgattc gtaccgccat gggtgagccg
    26881 gggccggatg gtcgccgtcg tcctcgtccg gttgcgggtt cagagtttga attgcccgcc
    26941 gatgttctca ttatggcctt tggtttccag gcgcatgcca tgccgtggtt gcagggcagc
    27001 ggaattaaac tcgataaatg gggcctgatt caaaccggcg acgttgggta tttacctacc
    27061 cagacgcatc tgaaaaaagt ctttgctggt ggggatgcag ttcatggcgc ggatctggtt
    27121 gtcactgcca tggccgcagg aaggcaggcg gcgcgcgata tgttaactct gtttgatacg
    27181 aaggcatcgt gatgaaatcg ttaattatcg ttaatccggc tgactgtatt ggctgccgta
    27241 cttgtgaagt ggcctgtgtg gtcgctcacc cttcagaaca ggagttgaat gccgatatct
    27301 ttttaccccg gctgaaggta cagcgactgg actgcatcag tgcgccggtg atgtgccacc
    27361 aatgtgaaaa cgccccttgt gttggcgctt gccccgtggg ggcgctgacg atgggcgagc
    27421 aggtggtgca gaccaattct gcccgttgta ttggctgtca gagttgcgtc agtgcgtgcc
    27481 cgtttggaat gatcaccatt cagtcgttgc tgggggatac ccggcaacaa atcgtgaaat
    27541 gcgatctctg tgaacagcgg gaagaggggc ctgcctgcgt tgaatcttgc ccaacacagg
    27601 cgctgcagtt gcttactgaa agagaactca ggcgagtccg ccagcagcgt attgttgcca
    27661 gcggtgagaa tcttctctga aagaaatctc cgcgctgaaa ggcgcggaga atctattaga
    27721 ttgcccaacc acccgcgtaa aatgcgacca gtgcggcagt aatgatcacc gtaccaatat
    27781 tcagcttttg ccattcgcgt gcaaagacgc ggcctacgac cagtgtcaca aagcccagca
    27841 taataccggt aacgatatta caagtcagaa cgatgaacac ggcacacacc aggccagcca
    27901 tagcgtcaat aaaatcattg aaatccagct tcgagacgtt acttaacatc agcaaaccta
    27961 cgtacatcag agcgggtgca gtggcgtaac cagggatcaa aaatgacagc ggtgataaga
    28021 acagaatcag caggaataac gcccccacta cggttgcggt taaccctgtt ttacccccgg
    28081 cggcggttcc tgccgctgat tcgatataaa ccgctgcggg cgctgcgccc accaggccgg
    28141 agaatattga acttacagag tcactggtca gggctttgcc gccgttgatg atctggttgt
    28201 ctttatccaa caaattcgcc tgaccggcga cggcacggat ggtgccagta gcgtcgaaca
    28261 ctgcggtcat caccaatgcc agtacactcg gaagtacagt tggctggagt gcgcccataa
    28321 tatcgaggct gaaaatcaga gacttaccat cttcgccagt caggcttggc atcgccacca
    28381 gaccgtggta tttcaccgct gggtcaaaga ttaagccgat gatcgaaatt gcaataatca
    28441 ccaacaagat cccgccgggt acgcgacact tctccagacc gaagatgacc gccagcccca
    28501 gcaagctcat catcaccggg aaggaggtaa acgcaccgag cgccactggc aagccttcaa
    28561 tcgggttttt gataaccata cccacaccgt tagcagcaat cagcagcaga aacagcccga
    28621 taccgatacc tgtaccgtga gcgataccca tcggcaaatt acgtaagatc caggtacgca
    28681 caccggttac ggaaatggcg gtgaagatga cgcccatcag aaataccgcg cccagtgcga
    28741 caggaacgct aatttgttgc ccgagtacca gactgaatgc ggtaaacgcc gtcaaggaaa
    28801 tcgcgcaacc aatcgccatt ggcaaattag cccataaccc catcagcaac gagccgaagc
    28861 ccgcgaccag acaggtggca acaaacacag ctgcgggagg aaaacctgct ttgcccagca
    28921 ttcccggaac gacgataacg gaataaacca tggccagaaa ggtcgttaag ccagccagta
    28981 cttcctgacg aacagtactg ccacgagcgg taattttaaa ataattatcc agcgcgccct
    29041 gtggctttgg tgcgtccggt gtttgtagga tgtctccaga cataatgatg tcctcacgag
    29101 gatgttaaag ttttattatt agttgcgttc gtacaccaga cgaccatcaa cgtaggtgcg
    29161 gtagatcgaa cggtcatcgc ccaacgtcat catcacgaac aatttgtcga ctaaagaaac
    29221 agagttgtca tagcgcagct gctgtagcgg agtggcggtg ggttccatca ccacgaaatc
    29281 agcctctttg ccaggtaaaa agttgccaat caaatcgtca aggcccagag atttcgctcc
    29341 gccgagcgtg gccaggtaaa acgcttcata tgccgagagg cgatagcctt gtaattgcaa
    29401 cactttgtag gcttcgttca gcgtttgcag catgttgaaa gtggttccgg caccgatatc
    29461 cgttcccatg cccactttaa ctttcttctg ccatgctttt ttcaagttga ataacccgct
    29521 gcccaggtaa aggttggagg tcggacagaa agcaatgctg gatttggttt cgctgagacg
    29581 atcccactct ttttcttcga gatggacgca gtgagcaaag acacagtttt taccggtcag
    29641 gccgtactga tggtaaacat ccagataacc atcatggtca ggataaagcg atttcaccca
    29701 ggcaatttca tctttgtttt cacagagatg ggtatgtacc cacgtatccg gatattcctc
    29761 tttcaggcgt tgcgccatcg ccatctgttc aggagatgag gtaggggcga atcgtggcgt
    29821 aatcgcatac aacagacgac catttttgtg ccagcgttcg atcagttctt tgctttggtg
    29881 atagctgctt tcggcagtgt cgagcagata atccggtgcg ttacggtcca tcatcacctt
    29941 accggcaatc atacgcatat tgatatgact ggcggcttca aacagcgcat caacggattg
    30001 cggatgaaca gtgccaaaca ccagcgccgt ggtggttccg ttacgtaaaa gctgcttgat
    30061 gaagaacgcc gacatttcgc gggcgtactc taaatcctca taacgacgtt cagtagggaa
    30121 ggtgtgttta ttcaaccact ccagcaattg ctcaccatag gcccccacca tttcactttg
    30181 cggataatgg atatgtgtat cgacaaagcc cggtactatc agtttgccgc gatagtcgcg
    30241 cacgcgaatg gtgtcaggaa tttgatgctt tccgttttcc cattcgccaa accattccac
    30301 ttttccctgt ttaatgagta ataaaccatc ctcaataaac cgcagcgcag aggcaatctc
    30361 ttccgggtta tcgaccgtac gggtgacatc aataaaactg cctcgtaccg cttttaacgt
    30421 gtgttctcct gacatcattg actcctttac tgttaatcca tctcttccgc tgaggtaata
    30481 ccaggcagaa cgttttcctg tcggtagcca ccagggagga taatatttaa taaaatcgca
    30541 gttaacccgc cagcacaaat agggttttca actaatacat aaatagaggc tggcaatatt
    30601 ttaaaaattt cgggatcgta ggagacgcca agccctaaac ctaaagaagt ggcgacaata
    30661 agtgtttcac gccgctttaa tccgttggtg atgatgatgc gaatcccagc gatggcaatc
    30721 atggaaaaca tcaacgtcat tgcgcctccc agaactgccg agggaatggt cgtgaagaag
    30781 ccgccaatca tcggaaataa gccgaggata accagcatta ccgcgatggt tcgcccgaca
    30841 taacgtgaag cgacgccagt catctgaata accccattat tttgcgcaaa cgtggttaat
    30901 ggtaatgaac caacagcgga ggcgataaca gaaaccagac catctgccag cacgccgcct
    30961 ttcagtcggg actgatactc ttccccctga atggggcggc gggaaaccat tgccgtggcg
    31021 gtgatatcgc cgactgcttc cagcacgcta agcagataaa tcgtgccaac caccaggaac
    31081 tgatggaagc taaaactaaa gccgtatttg aacggatgcg ggatggtgat taacggcaaa
    31141 ttgcgcatac tgctgaaatc caccatgccc aggcataacg atgcgatata gccgacacat
    31201 agcccaatgg cgatccctcc catgcgtagc aacggactgc gacagcagtt aaagccgatc
    31261 accacaatta aaaccaataa accaacgccg agatgttcgt aattgccgaa cgtaccgctg
    31321 cttttggctg caaatcctcc accaaaatcg ataatgccga ctttaatcag gcttaagccg
    31381 atcatcagta ccacaatacc gctgacggta ggcgtaataa cccgacgtaa atagggcaag
    31441 ataaatgaag atccgacaac cagaaatgcg ccaacgaagg agacgccgag aagcgacgac
    31501 atgattaact cttcgtgaaa accgtcgctt ttcatgctgc tgcccagcgc aatcatgacc
    31561 gtaacaaatg aaaaattgac tgactgaatt gagagtaggc cagaaccgac gatgccgtag
    31621 cggtttactt gtaaccaggt accaatacca gaggcgatca tcgccataga aacaagatag
    31681 gcggttgttt cagcggaaag ctgtaaggcc gcacccacga ttaacgcggg ggttaccatc
    31741 ggaacgaaaa ttgccaacag atgggtaatg gcaccaacga gagcctgatg aaagggaggg
    31801 cgatcctcca gttcaaatat aaggtcagaa cctgcatggt ttatatcaga catcccttca
    31861 ctccttttaa agtaaatttg atcatctgaa ttagtaaaat aaattcccac gaatttgggg
    31921 cgcaggacgc cttgagaacg gcttatctca ttttcgcagt cactatttag tcatcgttca
    31981 aacagagaaa caggccgccg aagcggccat ttattactta tattttttcc agcgcggtga
    32041 gtattttctc cggtgtgaaa tgccattcgc gtaaccagat gccgcaggca tcgtgaattg
    32101 cggtagcaat cgccggagct gcgccattta caccaatttc cgagatcgat ttcgccccga
    32161 acgggccgac tttatcgtcg ctcggcacca acacagcgcg gaaatcacgc ggaatgtcac
    32221 caattttcgg tgcgccataa ctgcgtaaat cacgcgttaa cgggtgacct tcggcgtcat
    32281 aaatgatctc ttcgctcata ctgtggccga tagcacgcag ggtggcaccg tagatttgtc
    32341 ccaacgctaa ctctggattg accggtgtac cgcagtccag caaggcgtag aatttatcca
    32401 ggcggatttc acccgtacgc gtgttgacgg caacttcagc gaagtttgcg ccatacggga
    32461 aagcgaaatc aggcgtgata taactgccag ttcccaccag tgaaccaaag ccggtgccgg
    32521 tttcgccttt atgggcaata tccccgaaac taacttcgcc tttcttgccg cgcacgacgc
    32581 ccggcgttgc tagttgaaca tctgccactg gctcacccaa catttgcgcg ccgtggaaca
    32641 gaattttctc ccgcagattt tccgctgcca aacgcgccgc gttacccgag aagcaagtac
    32701 cggacgaggc atatgcgcct ttatcaaaca acgcatgatc ggtatcaccg gagataacat
    32761 gcacgtcctg cggtgggcag tgcagcactt ctgctgccag tttcgtcact acagtatcca
    32821 gaccagtacc aatatccgca ccgccagaat gaacgataaa ggtgccgtcc gattccagtt
    32881 tgatcatgca gttagcctga tcgatatccg ggatacccga tttctgcatg ataatggcga
    32941 cgccgcgacc gatgtgccag tcaccatttt gcggtttcgg tgaagaccat tggatcatct
    33001 cgcgaccctg acgcaggatc tcttccagtg cgcagctggc ggcggaagga acggaggttg
    33061 gcgctttacc ttcaccaatt gcaccgagaa ttttcagctc ttgcccttcg tgtacccggt
    33121 tacgttcgat aatttccagt tggtcgatct gtaactgttc agcaagttcc gctaatgcca
    33181 tggtgatagc gaagttacct ttcggtgcgc cataaccctg ataagcacca tttgggcaaa
    33241 tgttgctgta gtaggtggtg acctggaaat cgacgttatc gcacggatat aagggcagcg
    33301 acagcgccgg tccgttacac ggtacggtga gtgagtggtt gccgtaaggg ccagtgttgg
    33361 cgcggaaatc catcttcact gccgtcaggc gaccatcttt tttcgctccc agtttgacgg
    33421 tgactttcgc gacgtgacga gaggtgttag caataaactc ttcttcacgg gtgtagcgga
    33481 acagtaccgg acgcccggtc acgcaggttg cccatgcgca cacttcttcc agcaggatgt
    33541 cctgtttgga accaaaaccg ccgccaactc gctctttaat gacatgaact ttatgctgtt
    33601 tcatgcccac gaggcgcgcg acctggcggc gtaagtgcca tggtacctgg gtggaggcgt
    33661 ggataaccag acgatcgcca tccatgcggg taaagcagat atgtgtttca gtcgggcact
    33721 gctgcgcctg cgttgagtta taggttcgct caatgatcac atcggcatcg gcaaagcctt
    33781 tgtccatatc gccaatatga ccatgaatac tggcggcgat atttttgcgg gggcgagaac
    33841 cgatcgggaa gttaatgatc atatgctcgc cgcgctgggc tgcatggctg ttatcgtctt
    33901 ccagagtatc tggcgcacca gcaacataca ccaccggttc atcgtgcacg acaggcgcat
    33961 cttccgccat tgcttcgtcg atcgacatta ccggcttaag cacttcatat tcaacgtcga
    34021 taagcttcag tgcttcgagc gcaatttctt cactttctgc gaccaccgca gcaacacgat
    34081 cgccgacgtg acgcattttc ttgccgaaca tacggcggtc aagcggtgac ggttccggtg
    34141 cgctctgccc ccccggggta tagtagatat ccgggcaatt cagatgagta ataacgtgaa
    34201 cgacgcccgg taaggcttca gctttgctga catccagatg agtaatcagt gcgtgagcgt
    34261 gtgggctacg taacatttta atgacgcagg cgtcagccgt tacgcggtct tcaacatagc
    34321 agggcttcgc ctgcaccatt tttgcggcat cagttttagg ataatgctta ccaatgactt
    34381 caaggtcgtc acggaaagtc ggagcgatat caatggtggc ctgcggatta tttttacgtg
    34441 caaccgccag ttcaatgacc tgatagtatt gctgccagcc agcatcgcgg ctaaacagac
    34501 ctgagagcgc gtcgtcgatc tcttcgcggg taggtgcggc gatgcgatcg agaagatcgg
    34561 tgataatcag agctgcagct ggatcgttat aaccagactg caccacgcca acatcaacca
    34621 ttgcctgttg aaccagactt aactcgttcc atttgcccag agattctgcg gtacgaatat
    34681 ctgccttctc taactgtgcg gcaataagca aggacgcgtt aacgatatta ccgttaaaga
    34741 taattgcgtc agaaccggca aacccgaagc catcatcact gttgcgtaca gagtgcattc
    34801 ccatgttaaa caacagcttt tgcacgtttt cgcctggatt aacggttagc tcctgaggcg
    34861 cgccatttaa agtaaagtgg atgatcatac ggcttcctcc ccagcctgtt ggcagtcggc
    34921 atacaggtcg gctaccagaa ctcccgtgat atagcgttta taggccacgc tgccccgcaa
    34981 atcttcctgc gggaaaatgg cgttggcgac agcctgttca agtgcattgc cttccagatt
    35041 ttgtttttcg acatcgtgca gacgcagtgc tttactggcg acgccatcca gcgcaattcg
    35101 cataccgtcg tggtctgtca ttgcaacggc tgccgtcacg acggttaaac ctgcctgaga
    35161 acggctaatt ttccgggtcg cacaggtgcg atacggatct ttaatgataa tttcggttag
    35221 caggcgatcg catgggcagg ccaggtagtc ctcgattgac agcgtttcgc cgttgccaaa
    35281 aaccagttca gcatccagtg ccagcaggac gggaagcagc accgactctt cctggcgggc
    35341 ggcgatttca ccaccaatgg tcgactgatt acgaacatgg cgtgagtaaa caaaaccgag
    35401 ggcttcacgc agcgctgcag gaataaatcg cgcatcacgc agtggctgca agcgagacat
    35461 tgcgccaatc cgcagtgcac cgttatccca gtcaacccag tccagttcca gatcctgtaa
    35521 ggagatggca atctttttat cggtacgggt tggtgtagca ttgagtttgc tgcccccggc
    35581 gaaccagacg gcttcatcct ggtagcggcg cttcagttcc agcgcctgtt cgacagagtc
    35641 gggcctgaaa aattgttcaa tcatcttctt tccttattta accctcttcc ctgcgggaag
    35701 agggcatctg tcatttatgc cagcgcatcc atccgacgcc acatactggc agcggctttt
    35761 ctggcttgtg cataaatgga atcgcaatcg aagttaaact gacggtcttc atagaccatc
    35821 acaccattga ccatcacgct gtgaacgctg cctgagccca taccgaaagc gatatgcccg
    35881 gcgatattgt ctgccagcag cggcgtcggc gagttgtaat cgcaaatggt taaatcagct
    35941 ttgtaaccgg ctttcagaag cccaaatttc gcgccaaagt tgcggctcat cagttcgtta
    36001 ccgttagtca gggctttggc aaaactgtca ggccacagcg gaccacccgc atcgcgatgt
    36061 ttaaagaagg caaatttcat ctcttcaaac atgtccgaac caatgccgtc cgttcccaac
    36121 gccagattgc ggatgtcgct aaggtgatgg ttgtagccga catggttgtt catgtttgaa
    36181 cgggcgttat gcaccaggaa cgcatcgcgc tgattgagta gggtgatgtc atctttcgac
    36241 aagtacagcc catgagcgac cagtgttttg ctgtcgatga gatcgaattg cgccagtcgt
    36301 gccagcaggt ctttgccgta ccagtggtga ctgtaggaaa cgtcgtaaag gtcttccgca
    36361 gcgtgaatat gcaaaccacg gcctgtggct ttcacggctt cacgcagcat ctccagaccg
    36421 gcatctggca cggtaaacgg cgcgtgagca ccgatatgtg cttccaccag atacggctcg
    36481 gttgctgctt tcttcgcctc atcaatctga cgggcaaagc ggatgttttc ttctacacct
    36541 tcctgcaact ctttgatgcc gttgttacgg tcagtagttt caaaacaggt catcgcgcgc
    36601 aggccaactt ttaaaaatgc gtcgcgcaat gtggagagcg acccgccgat atacgccgga
    36661 gaggcatggt gatcgataac cgatgtacat ccgctcttaa tcgcttccag ggaacaaatc
    36721 agtccgctgt aatagagcga ctcttcatca agggcgcgat cgagccgcca ccagagattt
    36781 ttcagcgttg agatgaaatc cgggcaaggg gcgatgtttg ccataattcc gcgggaaagc
    36841 cccgagtaaa aatggttgtg cgagcagaca attcccggca tcacaatccg accatgcatc
    36901 tctttgaagc tggcgtcggg gtagcgttgc gtcagggcat cgccgatagc gacaatcaca
    36961 tcgttttcga tggcgatatc aacgccttcc tgcacttttg ccgggtgtag ctgtaccgca
    37021 gtgacattct tcagaatcaa catgattaca cctccacgcg gcccagcaga taatgatgat
    37081 gctggtggac atggctgatg atgcggcaca tatcgttcag ctccggtggt acgttgttaa
    37141 actgaccttt gctgtcgatg tttaacaccc agctttggtt attcagacgt actcgtaccc
    37201 ggcaatcttc cacaaggaag cctgggttgc tgctgttatc aaagtcttgc gccaggctga
    37261 agacggtgat tttgtctttg tacggtttac cgttccacgg acagaactga gcgcagttgc
    37321 cgcattcgtt acagtaagcg tcgaggtgca gcgtctggaa acggttctgg aagcctggga
    37381 ccgcaatgga gacgttggcg cggttcgggc agacatccac acacttgctg caaacgtagt
    37441 tacattcgag gcagcgagcg gcttcctggg cgacaaacgc gtcacgatcg tcactgttca
    37501 ccagcgtgat agagatatcg cctttacgtt gatagatttc cgccggattg acgttgttcc
    37561 agtatttatc gttctggtgg gaacggatat tttcccgact aaggatggca tcggtcgccc
    37621 gacgcgcggt tccgacagca gcgacaatgg aggatggtcc gcgctgtacg tcgccgatca
    37681 taaagacgtc agtcagacga gtttcgccat tatggtcgac gtctggccag ccgtttttgt
    37741 ccagcggcac gcccatcgca ttcagggctt cagtatcctg ctgttcacca atggcggtga
    37801 tcaggctgtc tacatgcagt gttactgttt cgttggtttc aaccggacga cgacgacctt
    37861 tctcatccgg ttcgccaagc gacataacgc gcaaggttaa ggtgccatca gcatcgaaac
    37921 gttccggatt gttcaggaaa cggaactcca cgccgtcgtg caacgcttct tcatactctt
    37981 cgcgccatgc gggcatctct tgcagtgaac gacggtaaac gatcgttgct ttttctacgc
    38041 ctggaacgcg taacgccgca cgagcgcagt ccattgcggt gttacccgcc ccgacaacga
    38101 ccacatgttt gcccagcttg agcgctgtac ccttgttgta ttcacgcagg aaggggagtg
    38161 atttccagac attttggttg tcgcccgcca gtttcacacc gctattttta tcagtgccgg
    38221 tggcaatcag aacatagtgg aagccctgat tttttaactg ttcaacggtt aaatcgggtg
    38281 agcagccata ctcaaatttc acgccgtgat cggcaacaaa atcgatatcg tgctgaatta
    38341 actctgcagg aatacggaac tgaggaatga tatttttcac tacgccgccc gcattggctt
    38401 cgcgttcaaa cagcgtaacc ggatggcccg ctctggcaag gaagtaacct gcagccagac
    38461 ccgccggacc tgcaccaatc acggcaaccg gatggcgtga accagaaccg gctggtttgt
    38521 gccagcgttg cttatattca tcccaacctt tttccagcgc gacttttttc agttcgcgga
    38581 tattcagcgc actgtcgtaa tccaggcggg tacagttgta ttggcactgg tgatcgcaaa
    38641 tatgaccggt aatggcaggt agggcgttgc gttggtagat gagttccagc gcgtcggcat
    38701 agcggtgttc gccaagcaga cggatgtatt ccggaatatc ttgcttgata gcgcaggcag
    38761 taacacaggg ggcaacgtag cagtcggtca gcggcaggtc ttctgccact tcaatacgct
    38821 cttctggctt ccagtgtttc tgggtgtatt ccatggttaa cgcgtctgct gccagtctgt
    38881 tcagtcgttc gacgtcaaca tggtcaagtc cccaggcgtc ggagccttcc agctcgcgca
    38941 tgcaggcact taagcgcaga tagccgccag gtttcagcag atcggttgcc atagtaatag
    39001 ggcgaatacc agtatcaaaa atatcgcgga tagtcagctg actggcacca ccggaataag
    39061 aaatgggcag tttgccgtca aaggcgcgag agagaactgc tgcaacattg atggagagcg
    39121 ggaacagcgc acggcctgac atatacatct cttcaccggg cagtgcgcct ttattgttga
    39181 tggtgccgag agtgttagtc agttttacgc caaagccgag tgatttttct tttgccagtg
    39241 ccatcaggcg ttccagcatt tccagtgctt gcgtcagctt gaggtcgtga tcaaatgact
    39301 cttcttttaa gcctatgtaa ccgaaaccgc agacatcgag gatctcacga acacgcgcgt
    39361 acccgagtaa ggtcgggtta agtttgacaa aggtgttgag ccctttttct tccagcatgt
    39421 agcggcaaat ggcttcgatt tcatgcggag gacagccgtg catggtggag agggtgacgc
    39481 cctgcaccat actggtgggg atgcgagcgg gtaaagcttg caagctttcg cgtttttcct
    39541 gcaatccgtg gcgggctaaa aatgcgtcat cctggagtaa tttattcagc gtatcgcgat
    39601 attgcgcgaa tttcggatgg tcagatgcgt ccatcatatt gtcgatgaac tgttgcatcg
    39661 gcggttgctt aataccttca aggttgtaac cgacgctcat attaaagatg aacgatttac
    39721 cggaatcaga aggctggaac atcgcttcga gaaggtgcag ggcaaaccag gctttgaggt
    39781 attcatccca ggctttaagc agggtaaatt cggtagacca ttcggtgtta aagcactcgt
    39841 cttcggcatc gatacaaggc ttttccagct ccaggcggtc aagaatttgg acggttttta
    39901 gttcgatgaa gcgtccgcca gtcagccagg aagtgacaat attttgcgcg agctgcgtgt
    39961 gcgggccagc ggcagggccg acgggagtgg cacaggtttc accgaagacg ctaacagttt
    40021 taccttttac gggtgagtaa aactgttgct cgggaatacc aaagattgag cgttgttgtt
    40081 ggtattcatc aaatatgcgc gtcaaaagtt cctcaaacgg aatgggacgc ataatatccc
    40141 ccataaccct ctccttagct tatacagata tcattgagtg accggtgttg agaaatccgg
    40201 tggtttaggg ggatagagca acaatcgcac cactcttgcc aggtttggcg ggaaatataa
    40261 aaaaatgtga ggcggctcga aacttaaggg ctttttgtga atgaactcac aggtgccaaa
    40321 tgagaattaa aatcactcct gaaaattatt attgccagcc catttatcac tttttctctt
    40381 ttctcaaaat tagtgattcc ttatcattat gataaagagt gctggagggg ttaaatattt
    40441 gaacgcaatc gtttaactac gttttctaaa tttcagtata ccttttcttc gctgtaataa
    40501 agtcgtccgg agtatcaata tctaaaatta tttctgcatt ttctatttcg acggaataat
    40561 gatctcccat aaggagtgcc tgacgcatat tggtgacatt gggtcgctgg aatgcctgca
    40621 tcaagcatgg ttttgacact aaaatcggat ggccggggat gccattatgg agtggcagta
    40681 ttgcgccatc atttcgtaac gaccagattt ttctaaaaat atcaatggtg agggttggca
    40741 tgtcaccgtg ggtgagaaaa caatgttctg tttgcactgc gggtactgcg gccttgactg
    40801 atgttagtaa accctgggca taatctgggt tgtgaataat agtgatatta ctctggttcg
    40861 cataacgttc gtgcagttca ttaccacgat agccggtgac taaaataatt cggctacaaa
    40921 actgcaacgc atttttgata cttgtatcaa gaattgttcc ctgttgccag ggtaacatca
    40981 ttttccattg ccccattctt gatgataatc cggcagcggt aattatacag tcgatggctg
    41041 acatagtggt tccattattt ataggggcta ataacgcctg ataattgcgt tacttcctga
    41101 tggattaagt tgggggtaat ttatcccaga gaggtcataa agactcgtga aaagtatagt
    41161 tgacccatcg gcattattca ttgatttagg tgcgcagaaa cgtcccactg tgatttctgt
    41221 tgttggtgct ggggggaaaa ccagcttgct tttttggctg gcagaactgc tccagactcg
    41281 cggcaggcgt gtgttaatca ctacgacgac acatatgttt atgccaaagt cacactggcc
    41341 cgtggttttc tgtcgtgatc ccgccatgct tcctcatgcg tctcttacat cccccatttc
    41401 attttgtttt cacagctgga aagcgaacca gggaaaagtg cagggattta cgccagaagc
    41461 gattgatgca ctggtgcaac gaccagagtg tgacgtaatt ctcattgaag cagatggctc
    41521 gcgtggaatg ccgttaaaag cgcctgatga gcacgaacct tgcataccca aaagcagttg
    41581 ctgcgtgatt gctgtgatgg gaggacatac tttgggcgcg aaagtgagca cagaaaatgt
    41641 ccatcgctgg tcgcagtttg ctgatattac cgggttaaca cctgatgcaa ccttgcaact
    41701 gagcgatctc gttgcgctgg ttcgccaccc tcagggggcg tttaaaaacg taccgcaagg
    41761 ttgtcggcga gtctggttca ttaaccgttt ttctcaatgt gagaatgcga ttgcacaaag
    41821 cgagctcctt caaccgctgc aacaacatga cgtagaggca atctggctgg gcgatataca
    41881 agaacatcct gcaatcgcgc gcagatttgt gaattagcgg cccgggtatt ccgggttact
    41941 gaaaggatat ccattgaggt tggtatgaat attttcacag aggctgcaaa actcgaagag
    42001 caaaattgtc cgtttgcgat ggcgcaaatt gttgatagcc gaggctcgac tccccgccat
    42061 tctgcacaaa tgttagtgcg cgccgatggt tctatcgtcg gtacaattgg tggcggaatg
    42121 gttgagcgga aggtgattga agagtcgctt caggcattgc aggaacgtaa gccgcgatta
    42181 ttccatggac gtatggctcg taacggtgcg gatgctgtcg ggtcagattg tggaggtgca
    42241 atgtcagtat ttatcagcgt ccatggtatg cgtccacgtc tggtgttgat tggcgcgggg
    42301 catgtcaacc gggcgatagc ccagagtgcg gcgctattag gatttgatat tgccgttgct
    42361 gatatttatc gcgaaagcct caatcctgaa ctattcccac catcaaccac gcttctccat
    42421 gctgagtcgt ttggtgcggc agtggaagca ctggatattc gccctgataa ttttgtcctg
    42481 attgccacga ataatcagga tcgtgaagcc ctcgacaaac tcattgaaca gcccattgca
    42541 tggttggggt tgctggcaag tcgtcgcaag gttcagcttt tcctgcgtca attgcgtgag
    42601 aaaggcgtgg ctgaagaaca tattgcccgt ttacatgcgc ccgttggtta caacataggt
    42661 gcggaaacgc cgcaggagat cgccatcagc gtgctggcag aaatattaca ggtgaaaaat
    42721 aacgcgccgg gtgggctgat gatgaaacct tctcatcctt ccggacacca gctggtggtg
    42781 attcgcggtg cgggggatat cgccagtggt gtggcgctac gtctgtatca tgcgggtttt
    42841 aaagtgatca tgctggaagt ggaaaaaccg acagtgattc gttgtaccgt ggcgtttgcc
    42901 caggccgtgt tcgatggcga aatgacggtc gaaggcgtca ctgctcgcct gacaaccagc
    42961 tctgcggaag cgatgaaact taccgaacgc ggattcatcc ctgtgatggt agatcccgcc
    43021 tgttcattgc ttgatgaact gaaaccgctt tgcgtggtgg acgctattct ggcgaaacag
    43081 aatttgggaa cgcgggcaga tatggcacca gtaacaatcg cgcttgggcc tggctttact
    43141 gctgggaagg attgtcatgc ggtaattgaa acaaatcgcg ggcactggct cggtcaggtg
    43201 atttactctg gttgtgcgca ggagaatacc ggtgttcccg gcaatattat ggggcatacc
    43261 acccgacggg tgatccgtgc tcctgctgca ggcattatgc gatccaacgt gaaattaggc
    43321 gatctggtga aagagggcga tgtgattgcc tggattggtg agcatgaaat taaagcaccg
    43381 ttgacgggga tggtgcgtgg cttgttgaac gacggactgg cagtggtcgg tggttttaaa
    43441 attggtgata tcgatcctcg tggtgaaacg gctgatttca ccagcgtttc tgataaagcc
    43501 cgggcgattg gcggcggcgt acttgaagcg ttaatgatgt tgatgcatca gggcgttaaa
    43561 gcgacgaaag aagtgctgga agtggcttaa atgaaaatgg ccggatggta ttacccaccc
    43621 ggcctgagac tgatgacaaa cgccaaattg cctgatgcgc tacgcttatc aggcctacaa
    43681 gtgcattgca atctattgaa tttgcgatgt tttgtaggac ggataaggcg ctcgcgccgc
    43741 atccagcatc aacaaagcgc acttacttga caatctttgg ccggatggta ttacccaccc
    43801 ggccttaaaa ttactgacaa atcaccgttc cggttttgcc ttcaatccct tctttcgctt
    43861 tgctcaatac ggtaatcagt gcttcacgac ctgcgcgaga gcgagcaaac gaggcggcag
    43921 cttccacttt aggcagcata gaacctttcg caaaatgacc ttcttcgata aagcgttcag
    43981 catcacttaa tgacaggcga tcgagccact gttcattctc tttaccgaag ttaatggcca
    44041 ctttttctac cgcagtcaga atgatcagca tatcggcatc aatcatttct gctaaacggg
    44101 cgctggccca gtctttatcg ataaccgcgc tggcaccacg cagatggtta ccttcacgaa
    44161 taacagggat accaccaccg ccaacggtga tcaccacctg gcctgcatct accagagctt
    44221 taaccgtttc tttttcaatg atatcaaccg gttttggcga ggcaacaaca cggcgatagc
    44281 cgcgaccagc atcttctttc agagtgtaac cctgttttgt cagttgctct gcttcttgct
    44341 cggtaaagaa cgaaccgatc ggcttggtcg ggttgaggaa tgccggatcg ttagcgtcga
    44401 cttcgacctg ggtaaccagc gttgctacag gtttattaat gccccgagaa agcagttctt
    44461 ctcttaaagc gttttgtaga tcgtaaccaa tataaccctg gcttaatgca acacagacag
    44521 acatcggcag cattggcgag tgtgcttcag tttttgcggc agcttcaaag gcctggttaa
    44581 tcatgcccac ctgtggcccg ttaccatgag tgacgataac ttcatgtccc tgggcaatta
    44641 aatcaacaat tgcctgagaa gtaattttta ccgctttcat ttgcccggcc aggtcatcgc
    44701 ccagcgcatt cccgcccagg gcgagaacaa ttttcttact catttttcct cactcatcat
    44761 tcttgattgt gtatagatcc ctggcagtgg ttagagcacg ggagggacaa acggtttgcg
    44821 tcgcaggaaa cgacctcgcc cggctttgcc tgtaaaggtg ccgtcacaga aaatcgtttc
    44881 accacgggat aaggttctga caatcgcgcc ctgacaggta aaaccctccc atggcgagta
    44941 gtcggcgttg tcgtggagat ggcgatgctg aatttgttgg ctctgacgtg ggtcgataat
    45001 caccacgtcg ccatcggaac cgggcgctaa taatcctttt tgcggccaca ggccaaacag
    45061 cctggcgggc atcgcgctgg ttaattcaac aaaacgttcc ggtgttatac gtcccgtcat
    45121 cacgccactg gaaaacagta actgcatgcg gttttccaca ccgggtaagc catttgggca
    45181 gcgactgaaa tcgcctttag aaatttgcag gcgttgagcc attgagaacg tgcagtgatc
    45241 ggtcgccacc acgtcaatcg caccatcgct gatgccacac cacagtttgt cctgctcgcg
    45301 tacgttacgc agcggtgggc taagaatgaa cttcatgcca tcttctgtat cgtagctgcg
    45361 ttcgtccaac aggagatatt gtgggcaggt ttcaacccag actggctggt gattcgcacg
    45421 ggcaagacgc agataatcca gacctaagcc gttagacagg tgcacgatat agagcggggc
    45481 gttaccggca atttgtgcca ggttaatcat gcgggcgatg gcttccgctt cgcattccag
    45541 agggcgactc aaggcgtgat agcgcggcgc ggttaaccct gcggcgataa actccgcccg
    45601 cttgctggcg atagcagcat cattttccgg gtgcacggtg gtcagcgcgc cggattcatg
    45661 caagcggcgt aatgcctgca aaacttcgtc atcgttgagt ttgtattgat aggttaaata
    45721 gagtttaaaa ctgctcagtc cttcctcgac catcatcggg atttcgtcga ggatggcgtg
    45781 attaatgtgc tggatcacac cgtgaaagct gtagtcgatg accgccttat gggcggcata
    45841 gccacgataa acctccagtt gatggcgtaa ccgacagccg tttgggccaa atcccatatg
    45901 gtcaataatg gttgttgtac cgccacacgc agctgcgcgg gtaccggtaa aaaaatcatc
    45961 acaactgcgc gcgatgccga catcaatatt gaaatgcgta tggacatcca cgccgccagg
    46021 gaaaacgtaa cagccagtgg catcaatttc ttcatacggg agctgcggcg aaatattgtt
    46081 gcccaactgg cgaacaatcc cgctttcaat cagcaaatcc tgtttggctt gtccatctgc
    46141 gttaacgaca gtgccgtttt tgatcaatac gcgcatagca aactccaaaa accctccggc
    46201 gaaccggagg ggaataagtg aagagtaatt attcggttgc cagccagctt aacgggattg
    46261 cagcgtacat cgcggcacag gtcaccaagt gagatttcca ggttttttcg ttaggtgcat
    46321 gcgcttcagg ttctttaccc gggccaaagc cgatcaccgg aatgccgtga cggcccatga
    46381 tagatacgcc gttagttgag aaggtccact tatcaacaac cggcgctttg ccaaacagcc
    46441 cttcgtaggc attcaccagt gctttaacgg tgaagtgatc ttcttccact ttccaggtcg
    46501 ggaagtagca ttcggttggg taaaccaggc cagtccagga cggacggtcg tagttgtaca
    46561 tagaaacaac cgcgttagct ttctgtactg caggcagggc gcggatttcg tccagcgcgc
    46621 cttcccaggt ttcgccccaa gtcagacggc ggtcaataga gacggcgcag ctatctgcta
    46681 cagcgcaacg gcttggggat gtgaagaaga tttcagaaac ggtgagggtg cctttgccga
    46741 ggaattcgtc ataacccaga cgttgggaga gttcttgtaa ttcgccaaga atcggaccca
    46801 ttttgaaaat ggcgttgtca ccgcgttctg gcgcagaacc gtggcagcta acaccctgaa
    46861 catcaatacg aatttccata cgaccgcgct gaccacggta tacctggcag tcggttggtt
    46921 cggtactgac cacaaattcc gggcgaatgc cggattgttc aataatgtac tgccagcaca
    46981 gaccgtcgca gtcttcttcc tgaaccgtac cggtaaccag cagggtatat tcatcttcca
    47041 gaccgaggtc tttaatgatt ttaccggcat aaaccataga tgccatgccg ccttcctggt
    47101 cggaagtacc gcgaccaccg atcagctcat cagtttccat gccttcgtac ggatcgaagt
    47161 cccagttttt gatgttgcca atgccgacgg tatcgatatg agcgtccatt gccaccagac
    47221 gcgggccgtg gccgatataa ccgagaacgt tgcccatcgg gtcgatttca actttatcga
    47281 agccgacttt ttccatctct tctttaatac gatgtactac gcgtttctcg tcgcagcttt
    47341 cactgggaat agcaaccatg tcgcgcagga agcgagtcat gtccgcctgg taatcttttg
    47401 ctttttcaag aatcagtttg aatggaatat tcttagccat tatttttctc cagatttttt
    47461 actccccgtt ttgcagggag tagttccaat agggtgatta aggtgctaca gcgtgtttgc
    47521 cttcccagac aacttcgcgg tagtgcttca cgtcggtgtc gccttcagtg ctgataacca
    47581 gcaccacggc atctttgttc agcgccagtt tttccatcag gctttgacgt tgcgggtgat
    47641 aatgaaccgc cgcgagaacg cccaaaccga cagcgccgga ttcaccggag atgatgcgcg
    47701 ggtcgttgcc gtacggatta cccagcacgc gcatacctaa tgcggcaacg ctgtcctggc
    47761 aggagatgaa ttgggtggcg cagttacgta ggatttccca gcccagcggg ttaggttcgc
    47821 cacaggccag gcccgccatg atggtggcca tatcaccgcc aacgttgacg atgtcgcctt
    47881 tgacgccgga gcgataaata cagtcagctt tgtcaggttc aacaataatg ctgtgcagat
    47941 tttgcgggct aaagacgtcg accagataac ccagcacacc accggccatt gctccgacac
    48001 cggcttgcag cagaacgtgc gtcggggtta cgcccatttc acgcatttgc tcgacggctt
    48061 catctgccag ggttgcgtag ccttgcatga tccaggttgg aattttggtg taaccttccc
    48121 atgccgtgtc ctgtaccact tcccagccgt gctgctgcgc gtgttgcatg gtcaggcgaa
    48181 cggtatcgtc atagttcata tccgtgacga tgcactcggc accgaggttc agaatggcgt
    48241 caacgcgttc ctgagcagaa cctttcggca tgtaaatcac cgcattctgt ccgagttgct
    48301 gcgctgccca cgccacaccg cgcccgtggt tgccgtcggt ggtggtcgcg aaagtcattt
    48361 tttcgccgat ggcatttttc aggtgctcaa atgacagcgt ttcgatatca agatgatatt
    48421 tttcacacaa taattgagcg atagcgtacg caccgccgag cattttgaag gcgttcagac
    48481 cgaatcgttt tgattcgtct ttgacaagaa ttttcttcac accaaaaagg tttgcgagat
    48541 cgtccagcgc acaaagcggt gttgggcgat aaccagctat tttctggtgg aactggcgcg
    48601 ccagtttggc ctggctttgc gaaaagagcg gtgatgtttc gccgttgaaa aatttgttat
    48661 cggcgatatc aatcttcaat gagaaaacgg acatatccta tccttttggc ctctggaaat
    48721 agagggccga ccagctgatc ggcccgaggg gttatttcac gcgttcttgc gcttctttca
    48781 gcagttgctc gagcagtgca cctggtttgg cgtatttacg ggacaggatc atcgcggcga
    48841 tgatataagg cttccagctg gcttctttgt aggtagcgat acggtatttt tcgaatacgc
    48901 cttcagtcac ttcaccttct ttacaggata cgccgctgat atcagctggc aggcagtgca
    48961 tgtacagggc ttcgccatca cgggtcagtt ccatcatctc ttcagtacaa tgccagtctt
    49021 tgtgttgcgc gttctgtgcc agacactgtt tttccagtgc ttttaagcct tcgtgatcgt
    49081 tcgcacgcag caattcagta cgctcttcca tcactttgta aggtgcccat gacttcggat
    49141 aaacgatgtc tgcgtctttg aaggcttctt ccatgctggt gacctgacgg aagctaccgc
    49201 cggaggcttt agcattgttt ttagcgactt caacgacatc cgggatcagg tcgtagcctt
    49261 ccggatgggc cagggtgaca tccataccga agcgagtcat cagaccgatg atgccttgtg
    49321 gtacagagag cggtttgcca tagcttgggg agtaggccca ggtcatggcg atttttttac
    49381 ctttcaggtt ttccaatgaa ccaaagtgtt cacgtaacca tgccaggtca gccattgact
    49441 gagtcgggtg gtcaatatca cattgcaggt tcactaaagc cggacgttgc ggcagcacac
    49501 cctgcttgta accgtcatca agtgcagcgc caacttcacg catataggcg ttgcctgcgc
    49561 ccagatacat atcgtcgcga ataccaatag cgtcggcgca gaaggagatc atattggcgg
    49621 tttcacgcac ggtttcgccg tgagcgattt gtgatttgcc ttcgtcgaga tcttgctgtg
    49681 caaggccgag caggttaagc gcggaagcat aagagaagcg ggtacgggtg gagttgtcgc
    49741 ggaatacgga aatacctaat ccactattaa agactttggt tgagatgttt tcagcacgca
    49801 gtgcttttaa tgctgcggca acgtccagta cttgtttcag ttcatctggc gtctgttccc
    49861 acgttaacaa aaaatctttc tcgtgaaggt gagaggtcag cgaattgata tccttaatca
    49921 gctcattaac agttttcatc ataaagtccc ggtagtggaa tgacaggcaa agtggtttat
    49981 ttcactgtga cttgccaaag tgattggtgt tttcacagct atacctggga ctaacaataa
    50041 gtgtgccaga attatccacg gggctgcaaa agactaattt gacggataat gtgtggggtt
    50101 gatcacaaat tgatgctagc ttccggttgt ttgtaaaata cgaaaacgtt tggctttttt
    50161 tattgcgtta tttatttgct gctaagtatc agaaataaga taaaggggca ggctatccct
    50221 cgtcagtttg ataaactcac cgccgtaatt ctcatattga tatagaaatg tgatatctct
    50281 tccataattt cattagaaag tgataaaaat cttatgtttt tatcaaagcc aatttcgcct
    50341 gagttaatat catcatgatc tgtgaaccat caacgtcttc aatgatcgac ttttaagtaa
    50401 gtctggaagg taaagctatg gagcttgcta ctacgcagtc agtattgatg caaattcaac
    50461 cgacaattca gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag gttgagatcg
    50521 ttgatgaaaa cttgtgtcgc gtcgccggaa ctggcgcgta tgggaagttt cttggtcgcc
    50581 agttgagcgg caactcacgc ctgctccgcc acgtcctgga aacgaaaact gaaaaagttg
    50641 tgacacagtc tcgcttcgat cccctttgcg aaggttgcga tagtaaagaa aattgccgcg
    50701 aaaaagcatt tctgggtacg cctgtcattt tacaggatcg ttgtgttggg gtgataagtt
    50761 tgattgccgt tacccacgag caacaagagc atatcagtga taatttacgc gaattttctg
    50821 attatgttcg ccatatatcc accatttttg tttcgaaact tctggaggat caggggccag
    50881 gagataacat cagtaaaata ttcgcgacca tgatcgataa tatggatcag ggcgtattag
    50941 ttgttgatga tgaaagtcgg gttcagtttg ttaatcagac tgccttaaaa acacttggta
    51001 ttgtacaaaa taatattatt gggaaaccta tccgtttcag accattaaca tttgagagta
    51061 attttaccca tggacatatg cagcatattg tttcgtggga cgataaaagt gaattaatca
    51121 ttggccaatt gcataacatt cagggccgac aattattttt aatggcgttt caccaatcgc
    51181 ataccagttt ttctgtagca aatgcacctg atgaaccgca tattgaacaa ttggttggcg
    51241 agtgccgtgt tatgcggcaa ttaaaacgac tcattagccg tattgcaccc agcccatcca
    51301 gcgttatggt ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccgt gcaatccata
    51361 agttgagcgg aagacggaat aaacccttta ttgctatcaa ctgtgccgcg attccggagc
    51421 agcttctgga gagcgaactg ttcggttatg ttaaaggcgc atttactggc gcttctgcca
    51481 acggtaaaac agggttgatt caggcggcga atacgggcac gctgtttctc gatgaaatag
    51541 gtgatatgcc attaatgttg caggctaaat tactgcgcgc tattgaggca cgtgaaattc
    51601 tgccgattgg tgccagtagc ccaatacaag tcgacattcg catcatttct gcaactaacc
    51661 agaatttggc ccagttcatt gccgaaggta aattccgcga agatctcttc taccgactta
    51721 atgttatccc gataactctg ccaccgctgc gtgaacgtca ggaagatatt gaactactgg
    51781 tgcattactt tttacatctg catacccgtc gtctgggatc ggtttatcct ggcattgctc
    51841 ccgatgtcgt cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc gagttaagca
    51901 atttgatgga atatctggtt aacgttgttc cttcaggtga agttatcgac agcacgctat
    51961 tgccgccaaa tctgctgaat aatggcacaa cggagcaaag tgatgtaaca gaggtcactg
    52021 aggcacacct gtcactcgat gatgcgggcg gcacggcgct ggaggagatg gaaaagcaaa
    52081 tgatccgcga ggcgctttca cgtcataaca gcaagaagca agttgctgat gaactgggca
    52141 tcggcattgc tacgctctat cgcaagatta agaaatatga gttgttaaac acataacatc
    52201 agtgaatccg gcctgaattc aggccggata tccttttact tcgttttctc gcaatccaga
    52261 actgtattta caatcatctg ataccccgtg cagcgacaaa gatttcccgc cagtccgcga
    52321 cgaatttccg taatggttaa tggcttctcg cgtggtttcg ccagcattgc cgtggtagcc
    52381 ataatcaggc caggcgtaca aaacccgcac tgcactgcgc cggatttcgc ataagcctgc
    52441 tgaacatgag aaagttttcc gcctttcgct tcaccttcca gcgtgcggat ctcttttcct
    52501 tcagcccagg cggcaaggta taagcaactg tctattgctg tgccgtcgac cagcaccgta
    52561 caggcaccac attcacccac gcagcaccct tgtttgacac ttagcagtcc ttgttcgcgg
    52621 agtaattccg agagcggcgt gcctggtgcg gcatgaagct gaaaaggcat cccgttaatg
    52681 gtgcattcga tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc gccgcggcga
    52741 cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga aactctttac
    52801 tggcccgcca tgaagaacgc ggggcgacat cttgcaggac agattcgctg atagcttcca
    52861 gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg cagcgaatcg
    52921 gcgttggcgc ggcaacacca aatgccagac gtaattcgct gaaattgccg ttatccagtc
    52981 ggcaatgtgc ggcgcagcca atcgttgaaa tatccattgc gtcgcgcatg gcatatttaa
    53041 aatgcgcgct gcccgcgtgt tctttcggct ggggcggaaa atgaaaggcg acaaggattt
    53101 cgtcatgctc aagagacact ttgcctggcc cagtgtgaaa gccattaatc gggacgaaac
    53161 gaacaccgcg tggggagtgg atctccagct ttgcgtcata aattagcgtt ggcgtggcag
    53221 aatctgcgct ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg ttacggatct
    53281 gcggtccggc aatggacgag gccgcagcac ataacgccgg gagatgacgt tgagttatag
    53341 aatcttctat tagctgggta aatgtcgttg cagagccgat acgtagcgag ccatcttccg
    53401 ccagcgtaat tccccgcagc tccgccagat tatgaatatc aacaatatgg cgataacggt
    53461 cattgtgatg gtggagctga atcagtacgt cagtgccacc ggcgagcagt ttggcctgcg
    53521 ggttgtcagc cagcaggttg atggcatcgg caagggttgc tgcgcgatgg taagaagcaa
    53581 aatcaaacat gatgttatcc tcaaatcaat cctgccagat gaaactcttc atataaccgt
    53641 tttggcgtca gcggcagtgt attgattgca acaccggtag ccatcttcac cgcgttacga
    53701 atagctgcgg caacaggaat tattggtggc tcacccagtg acttatgtcc gtatgcggat
    53761 tgcggttcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg catggtcggc
    53821 attttgtaat ccagcagatt ggggttacgg accacgccgc ttttcgcatc gatgatcatc
    53881 tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg accttctgcc
    53941 agcagcggat ttagaatatg ccccgaatca tgaacgttga ggatgcggtt gatggtgact
    54001 ttgcacagtg caatatcgac cgtcagatca acaaaggtac agccaaacgc cggtgggtta
    54061 gtggtggttt tgatggagct ttcagcagag agctgcccgc cgcgttcagg gtggtagaaa
    54121 gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc aaccagcacg
    54181 atatggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac tgcggcgtga
    54241 gcgatgattt tctcttttaa taacagtgcc gcactgcgca gcgcaggcgc ggcaacatag
    54301 ctctggcgtg aggcaaatgc gccgggatcg aacggcgtaa cgtcggtatc ttgtgttgaa
    54361 ataacgcgaa cgtcgctaac cggaaccccc acggtttctg ccaccatttg cgagaagacg
    54421 gtgtcggcac cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt tccatcctga
    54481 ttcatcagaa ggcgcgcgcc tgctatttct acgccgacag gccaggtatt agaggtgtag
    54541 ctaaaacagg cgacgccaac gccgcggcgc aaattgcctt gctggttctg gcattctgca
    54601 cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg caaccctgcg
    54661 ctgtaaatac gtttgcccgt gagcggatta gcatctcctt cgcgggcggc gttgcgtaaa
    54721 cgaatttcaa caggatcaat acctaacgct gtcgcggcgt catcaagcat agactcaacg
    54781 gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg gaggttggta
    54841 tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc gactttattc
    54901 ccccccgcag aagcgatgga gtgcccgtga gatgcataag cgccggtgtt agacagaaca
    54961 tccagactat aacctttcaa tgttccgtcg cggctcacgc ccatttgccc gtcaatagta
    55021 aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga aactttcacc
    55081 ggaatgccgc caagcttgct ggtcaggaat gccgccattg gctcttccag tacatcctgt
    55141 ttattaccaa aaccgccacc gacaaatggt ttgatgactc gtacgcatga ccagggaata
    55201 tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct ggaaacgatg
    55261 gtaattcgcg agtcatcctc catccacgcc agcgatgtta cgctttccat gtgacaatgt
    55321 tgaataacgg gggtctgata gtgcccctgt acctggtagt cggcggcatc gattgtttgt
    55381 tggacattgc ccgtcgacat cgtgctttgt ttcagtaaat tgccgccgtt atggattggt
    55441 gcagcgtcct ctgccagcgc cgcttctggc gtggtgataa cgggtaattc ttgccactca
    55501 atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac gacgatggca
    55561 acggcgtcac catgatgacg aacatggcga gttagcagtg cgcgatcggc ggtatcgcgc
    55621 ttgttttcgt caagtgtcca tgcatgccca gctgtagcga atgggatatc aggcacatct
    55681 tcccaggtaa aaatcgccag tacgcctggt aaacttctgg cttgttcatc attaatactt
    55741 acggcataac catgtgcgat agggctacgt acatatttcg cataacacat gcccgccata
    55801 acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc aacgcgcatg
    55861 catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc gatttgaaca
    55921 tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag aaacgacgat
    55981 aagcgttagg ggaggaagga agtgtgatga cgttaaaaaa acacagatat gatacgaaac
    56041 aggactctga ataggggcag tgatacgtat caatatgagt ggcgataaaa tcaatgtgat
    56101 aaattgggat tattacgcat caggatggga aatagggtaa aaaagtcagg aaattcgata
    56161 cctctacagc gaaaattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt
    56221 ctctattacc gcgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga
    56281 tatggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa
    56341 tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc
    56401 aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg
    56461 ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag agctggcgcg actgaacggc
    56521 atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt
    56581 agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg
    56641 tcatcagcgg taccgaaatc ttcctggccg ccagtagggc aacgttgttg gttatggcca
    56701 acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat
    56761 atctctgctc cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg
    56821 ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt
    56881 acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg
    56941 caaaaaatcg ccaccatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt
    57001 cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca
    57061 aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa
    57121 ggtttataat gccttacgca tctcaaagcg ggcgtagttc aatggtagaa cgagagcttc
    57181 ccaagctcta tacgagggtt cgattccctt cgcccgctcc aatttatctt cacctcatca
    57241 acctgccatt tttgttgtat ctgattttct atattatctt aattcaatcg gttcagactt
    57301 ttcgcaatcg cagtaacact tccccatttt tttgtaaata aaaagcgttt ac
//
LOCUS       DAZGCQ010000018        56184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000018 DAZGCQ010000000
VERSION     DAZGCQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.018"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /gene="yneK"
                     /locus_tag="ACHK07_001190"
     CDS             <1..840
                     /gene="yneK"
                     /locus_tag="ACHK07_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO8101550.1"
                     /translation="ARNGRHKENIEFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVT
                     MGRVFQTGVERVLFLFLNDFIEQFPMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQF
                     DLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKEDLLQSLMD
                     SLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFASMLLHYHQFMNPNESDLNNVLM
                     PASLSDDKIIKHMARKTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDIP
                     DRH"
     gene            991..2181
                     /gene="ydeA"
                     /locus_tag="ACHK07_001191"
     CDS             991..2181
                     /gene="ydeA"
                     /locus_tag="ACHK07_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO8101551.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2206..2871)
                     /gene="marC"
                     /locus_tag="ACHK07_001192"
     CDS             complement(2206..2871)
                     /gene="marC"
                     /locus_tag="ACHK07_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO8101552.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3083..3517
                     /gene="marR"
                     /locus_tag="ACHK07_001193"
     CDS             3083..3517
                     /gene="marR"
                     /locus_tag="ACHK07_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO8101553.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3538..3921
                     /gene="marA"
                     /locus_tag="ACHK07_001194"
     CDS             3538..3921
                     /gene="marA"
                     /locus_tag="ACHK07_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO8101554.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3953..4171
                     /gene="marB"
                     /locus_tag="ACHK07_001195"
     CDS             3953..4171
                     /gene="marB"
                     /locus_tag="ACHK07_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO8101555.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4202..5101)
                     /gene="eamA"
                     /locus_tag="ACHK07_001196"
     CDS             complement(4202..5101)
                     /gene="eamA"
                     /locus_tag="ACHK07_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO8101556.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGALLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            5296..6483
                     /gene="ydeE"
                     /locus_tag="ACHK07_001197"
     CDS             5296..6483
                     /gene="ydeE"
                     /locus_tag="ACHK07_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO8101557.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWRQP
                     ALC"
     gene            6610..6705
                     /gene="mgtS"
                     /locus_tag="ACHK07_001198"
     CDS             6610..6705
                     /gene="mgtS"
                     /locus_tag="ACHK07_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO8101558.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6705..6810
                     /gene="mgtT"
                     /locus_tag="ACHK07_001199"
                     /pseudo
     CDS             6705..6810
                     /gene="mgtT"
                     /locus_tag="ACHK07_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
     gene            complement(6925..7815)
                     /gene="dgcZ"
                     /locus_tag="ACHK07_001200"
     CDS             complement(6925..7815)
                     /gene="dgcZ"
                     /locus_tag="ACHK07_001200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO8101559.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FAAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(8069..8461)
                     /gene="ydeI"
                     /locus_tag="ACHK07_001201"
     CDS             complement(8069..8461)
                     /gene="ydeI"
                     /locus_tag="ACHK07_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO8101560.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            8737..9255
                     /gene="ydeJ"
                     /locus_tag="ACHK07_001202"
     CDS             8737..9255
                     /gene="ydeJ"
                     /locus_tag="ACHK07_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO8101561.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(9300..11345)
                     /gene="dcp"
                     /locus_tag="ACHK07_001203"
     CDS             complement(9300..11345)
                     /gene="dcp"
                     /locus_tag="ACHK07_001203"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO8101562.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGSLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWGFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDEVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            11482..12228
                     /gene="ydfG"
                     /locus_tag="ACHK07_001204"
     CDS             11482..12228
                     /gene="ydfG"
                     /locus_tag="ACHK07_001204"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO8101563.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            12317..13003
                     /gene="rspR"
                     /locus_tag="ACHK07_001205"
                     /gene_synonym="ydfH"
     CDS             12317..13003
                     /gene="rspR"
                     /locus_tag="ACHK07_001205"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO8101564.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            13180..13383
                     /gene="ydfZ"
                     /locus_tag="ACHK07_001206"
     CDS             13180..13383
                     /gene="ydfZ"
                     /locus_tag="ACHK07_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO8101565.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(13418..14878)
                     /gene="ydfI"
                     /locus_tag="ACHK07_001207"
     CDS             complement(13418..14878)
                     /gene="ydfI"
                     /locus_tag="ACHK07_001207"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8101566.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLFLLAHGAKATVAKYSVK"
     gene            complement(14968..16335)
                     /gene="ydfJ"
                     /locus_tag="ACHK07_001208"
     CDS             complement(14968..16335)
                     /gene="ydfJ"
                     /locus_tag="ACHK07_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO8101567.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(16393..17412)
                     /gene="rspB"
                     /locus_tag="ACHK07_001209"
     CDS             complement(16393..17412)
                     /gene="rspB"
                     /locus_tag="ACHK07_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO8101568.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(17424..18638)
                     /gene="rspA"
                     /locus_tag="ACHK07_001210"
     CDS             complement(17424..18638)
                     /gene="rspA"
                     /locus_tag="ACHK07_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO8101569.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTITPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(18844..19170)
                     /gene="ynfA"
                     /locus_tag="ACHK07_001211"
     CDS             complement(18844..19170)
                     /gene="ynfA"
                     /locus_tag="ACHK07_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO8101570.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            19305..19646
                     /gene="ynfB"
                     /locus_tag="ACHK07_001212"
     CDS             19305..19646
                     /gene="ynfB"
                     /locus_tag="ACHK07_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO8101571.1"
                     /translation="MKITLSKRIGLLAFLLPCTLALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            19681..20241
                     /gene="speG"
                     /locus_tag="ACHK07_001213"
     CDS             19681..20241
                     /gene="speG"
                     /locus_tag="ACHK07_001213"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO8101572.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(20244..20954)
                     /gene="ynfC"
                     /locus_tag="ACHK07_001214"
     CDS             complement(20244..20954)
                     /gene="ynfC"
                     /locus_tag="ACHK07_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO8101573.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            21062..21367
                     /gene="ynfD"
                     /locus_tag="ACHK07_001215"
     CDS             21062..21367
                     /gene="ynfD"
                     /locus_tag="ACHK07_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO8101574.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            21565..23991
                     /gene="ynfE"
                     /locus_tag="ACHK07_001216"
     CDS             21565..23991
                     /gene="ynfE"
                     /locus_tag="ACHK07_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO8101575.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            24052..26475
                     /gene="ynfF"
                     /locus_tag="ACHK07_001217"
     CDS             24052..26475
                     /gene="ynfF"
                     /locus_tag="ACHK07_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO8101576.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPKMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            26486..27103
                     /gene="ynfG"
                     /locus_tag="ACHK07_001218"
     CDS             26486..27103
                     /gene="ynfG"
                     /locus_tag="ACHK07_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO8101577.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            27105..27959
                     /gene="ynfH"
                     /locus_tag="ACHK07_001219"
     CDS             27105..27959
                     /gene="ynfH"
                     /locus_tag="ACHK07_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO8101578.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            28002..28616
                     /gene="dmsD"
                     /locus_tag="ACHK07_001220"
     CDS             28002..28616
                     /gene="dmsD"
                     /locus_tag="ACHK07_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO8101579.1"
                     /translation="MTHFSQQDNFSVVARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGKLARLTLAQWQSQLLIPVAVKPLFR"
     gene            28777..30069
                     /gene="clcB"
                     /locus_tag="ACHK07_001221"
     CDS             28777..30069
                     /gene="clcB"
                     /locus_tag="ACHK07_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO8101580.1"
                     /translation="MHHLHIYPNLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(30022..30717)
                     /gene="bioD"
                     /locus_tag="ACHK07_001222"
     CDS             complement(30022..30717)
                     /gene="bioD"
                     /locus_tag="ACHK07_001222"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8101581.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(30842..32062)
                     /gene="mlc"
                     /locus_tag="ACHK07_001223"
                     /gene_synonym="dgsA"
     CDS             complement(30842..32062)
                     /gene="mlc"
                     /locus_tag="ACHK07_001223"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO8101582.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(32197..33090)
                     /gene="ynfL"
                     /locus_tag="ACHK07_001224"
     CDS             complement(32197..33090)
                     /gene="ynfL"
                     /locus_tag="ACHK07_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8101583.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            33197..34450
                     /gene="ynfM"
                     /locus_tag="ACHK07_001225"
     CDS             33197..34450
                     /gene="ynfM"
                     /locus_tag="ACHK07_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101584.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(34499..34618)
                     /locus_tag="ACHK07_001226"
     CDS             complement(34499..34618)
                     /locus_tag="ACHK07_001226"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO8101585.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            34874..35182
                     /gene="asr"
                     /locus_tag="ACHK07_001227"
     CDS             34874..35182
                     /gene="asr"
                     /locus_tag="ACHK07_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO8101586.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            35275..35358
                     /locus_tag="ACHK07_001228"
     CDS             35275..35358
                     /locus_tag="ACHK07_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101587.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            35458..36279
                     /gene="ydgD"
                     /locus_tag="ACHK07_001229"
     CDS             35458..36279
                     /gene="ydgD"
                     /locus_tag="ACHK07_001229"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO8101588.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36318..36647)
                     /gene="mdtI"
                     /locus_tag="ACHK07_001230"
     CDS             complement(36318..36647)
                     /gene="mdtI"
                     /locus_tag="ACHK07_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO8101589.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(36634..36999)
                     /gene="mdtJ"
                     /locus_tag="ACHK07_001231"
     CDS             complement(36634..36999)
                     /gene="mdtJ"
                     /locus_tag="ACHK07_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO8101590.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            37411..38445
                     /gene="tqsA"
                     /locus_tag="ACHK07_001232"
     CDS             37411..38445
                     /gene="tqsA"
                     /locus_tag="ACHK07_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO8101591.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(38470..39858)
                     /gene="pntB"
                     /locus_tag="ACHK07_001233"
     CDS             complement(38470..39858)
                     /gene="pntB"
                     /locus_tag="ACHK07_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO8101592.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(39869..41401)
                     /gene="pntA"
                     /locus_tag="ACHK07_001234"
     CDS             complement(39869..41401)
                     /gene="pntA"
                     /locus_tag="ACHK07_001234"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO8101593.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            41925..42869
                     /gene="ydgH"
                     /locus_tag="ACHK07_001235"
     CDS             41925..42869
                     /gene="ydgH"
                     /locus_tag="ACHK07_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO8101594.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            43055..44437
                     /gene="ydgI"
                     /locus_tag="ACHK07_001236"
     CDS             43055..44437
                     /gene="ydgI"
                     /locus_tag="ACHK07_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8101595.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            44474..45196
                     /gene="folM"
                     /locus_tag="ACHK07_001237"
     CDS             44474..45196
                     /gene="folM"
                     /locus_tag="ACHK07_001237"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO8101596.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(45193..45528)
                     /gene="ydgC"
                     /locus_tag="ACHK07_001238"
     CDS             complement(45193..45528)
                     /gene="ydgC"
                     /locus_tag="ACHK07_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO8101597.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            45657..46376
                     /gene="rstA"
                     /locus_tag="ACHK07_001239"
     CDS             45657..46376
                     /gene="rstA"
                     /locus_tag="ACHK07_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO8101598.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            46380..47681
                     /gene="rstB"
                     /locus_tag="ACHK07_001240"
     CDS             46380..47681
                     /gene="rstB"
                     /locus_tag="ACHK07_001240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO8101599.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            47757..48686
                     /gene="tus"
                     /locus_tag="ACHK07_001241"
     CDS             47757..48686
                     /gene="tus"
                     /locus_tag="ACHK07_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO8101600.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHNPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(48683..50086)
                     /gene="fumC"
                     /locus_tag="ACHK07_001242"
     CDS             complement(48683..50086)
                     /gene="fumC"
                     /locus_tag="ACHK07_001242"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO8101601.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(50229..51875)
                     /gene="fumB"
                     /locus_tag="ACHK07_001243"
     CDS             complement(50229..51875)
                     /gene="fumB"
                     /locus_tag="ACHK07_001243"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8101602.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            52074..53249
                     /gene="manA"
                     /locus_tag="ACHK07_001244"
     CDS             52074..53249
                     /gene="manA"
                     /locus_tag="ACHK07_001244"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO8101603.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIL
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            53350..54858
                     /gene="ydgA"
                     /locus_tag="ACHK07_001245"
     CDS             53350..54858
                     /gene="ydgA"
                     /locus_tag="ACHK07_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8101604.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(54903..56168)
                     /gene="uidC"
                     /locus_tag="ACHK07_001246"
     CDS             complement(54903..56168)
                     /gene="uidC"
                     /locus_tag="ACHK07_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO8101605.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT        14277 a        14305 c        13370 g        14232 t
ORIGIN      
        1 gccaggaatg gaagacacaa agaaaatatt gaatttcagg tagtaataaa tttaagccct
       61 atttatttag ataatttccg ccatgatgga gaaattaata ttttttgcgc tcccaaccca
      121 aagcctgtca ctatggggcg cgtatttcag accggcgtcg agcgtgtgct ctttctgttt
      181 ctgaatgatt ttattgaaca atttccaatg ataaaccctg gcgttcccat caaaagagcg
      241 catacaccac atattgaacc cctgcctccg gatcaccata ccgctgcaga ttacttacgc
      301 cagtttgatt tgcttgtcct gaattttatc tctcgcggta attttgttat actcccccga
      361 ttatggaata actctgaggt tcacagatgg ttcgtcaata aagatcctaa cttgatcacc
      421 gccattctcg acataacgga cagcgaatta aaagaggatt tattgcaaag cctgatggat
      481 tcattaggtt ctaacaaaca tgtactaccc gaagtctgca tctgcttttt atccctttta
      541 gcagaacaag aatctcctca ttttcaggac ttgtttttat ttttcgccag tatgttattg
      601 cactatcacc aatttatgaa tcccaatgaa agtgatttga ataacgtgtt gatgccagca
      661 tcattaagtg atgataaaat tatcaaacat atggcgcgca agaccctcaa actttttgta
      721 aaaaatgaaa caccgccaaa agttactcat gaagacctgg tgaaaaacag gcctcgctcc
      781 cctgtcagac cacctatacc cgcaacctcc aaaacgccag acattcctga tcgtcattaa
      841 accgtgatgt ttaccgtctc tctgacgcgt gaaagaatca gcgtcagaga aacggaaaac
      901 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
      961 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
     1021 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
     1081 gtccctgttg gcctactctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
     1141 ggcattatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgcta
     1201 atgaccagcc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     1261 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
     1321 gtggcttttg cacatgcgat tttctggtca attacggcgt ccctggcgat ccgtatggct
     1381 ccggccggga agcgagcaca ggcattgagt ttaattgcca caggtacagc actggcaatg
     1441 gtattaggat tacctctcgg gcgcattgtg gggcagtatt tcggttggcg aatgaccttc
     1501 ttcgcgattg gtatcggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta
     1561 ctgcccagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     1621 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     1681 tatattgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     1741 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcgggaa actgggtaat
     1801 cagtatgcgt ctgcgttggt gagtacggcg attgcgctgt tgctggtgtg tctggcactg
     1861 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
     1921 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgcc
     1981 accgacgtcg cgatggcgct attctccggg atatttaata ttggaatcgg ggcgggtgcg
     2041 ttggtaggta atcaggtgag tctgcactgg tcaatgtcga tgattggtta tgtgggcgcg
     2101 gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
     2161 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
     2221 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc
     2281 catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga
     2341 gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
     2401 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
     2461 catcgcaatg gttcccggac cggcggtact tggcattgcc agtggtacaa aagcaatatt
     2521 ggcgctgggt tcatcttcca gctcttccga cttgcttttc gcttccggtg aatcaatcgc
     2581 tttctgttgc ggaaagagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
     2641 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc
     2701 caccatcatg atggcaaaga cgtacaccga ggccatcaac gactgacgat tacgttcggc
     2761 actgttcatg ttgcctgcca ggccaagaaa taacgcgaca gttgttaatg ggttagctaa
     2821 cggcagtaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
     2881 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
     2941 actctttagc taaccttgca tcgcattggc caaaacttga accgatttag caaaacgtgg
     3001 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattatcc tctgcaacta
     3061 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
     3121 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta atgagtatct
     3181 gtctccgctg gatattactg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
     3241 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
     3301 tatgctggat cgcctggtct gtaaaggctg ggtggaaagg ttgccgaacc cgaatgataa
     3361 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgtgaac aatgccatca
     3421 attagttggc caggacctgc accaagaatt aacaaaaaac ctgacggcgg acgaagtggc
     3481 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaaagagg tatgacgatg
     3541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     3661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     3721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     3781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     3841 gtcccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     3901 ttaaaccatt acaacagcta gttgaaaacg tgacaacgtc actgaggcaa ttatgaaacc
     3961 gcttttatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
     4021 aacccagcaa ccgctcgtta cttcctgtgg cgatgtggtg gttgttcccc catcgcagga
     4081 acaaccaccg ttcgatttaa atcacatggg tacaggcagt gacaaatcgg atgcgctggg
     4141 cgtgccctat tataaccaac aagccatgta gtatattcag gccccggcat atcggggctt
     4201 gttaacctct tacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
     4261 cggtcataat gagcaccgcg ccgaaaaatt gcagacccgt taagcgttca tccaacaata
     4321 gtgccgcact tgccagtccg actacaggca ccagtaacga taacggtgca acccgccagg
     4381 tttcatagcg tccaagtagt gctccccaga tcccataacc aacaattgtc gccacaaaag
     4441 ccagatacat cagagacaag atagtggtca tatcgatagt gaccagactg tgaatcatgg
     4501 ttgcggaacc atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaatgcgc
     4561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
     4621 agatgttgcc acacgcccaa ctaaatgctg ccgccagggt caacataaag ccgagcatcg
     4681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
     4741 cgatccccgc caattgtttg ccatgcagtc gctcaccgaa agtaaacgcg ccaagcacga
     4801 tagtaaaaaa cgcctgagcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
     4861 taatggcgca gaagagaaag gcaaactgtg caaaactgat ggttaatcca taccccagca
     4921 gcaaattcag tggtactttc ggtcgtgcga caaaaaagat ggccggaaaa gcgaccagca
     4981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
     5041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
     5101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
     5161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
     5221 acttcggatt tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca
     5281 ctgccggaca gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg
     5341 tcgttgttat taaccatcgg acgcggcgct acgctgccat ttatgaccat ttacttaagt
     5401 cgtcagtaca gcctgagtgt cgatctcatt ggttatgcga tgacaattgc gctcactatt
     5461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
     5521 atgttactgg caattacggc cttcgccagc ggttttattg ctattcccct ggtggacagc
     5581 gtcacgctgg tggtactttt tttcgcactc atcaactgcg cctattccgt ttttgccaca
     5641 gtgttgaaag cctggtttgc cgacaatctt tcatccacca gcaaaacgaa gatcttctcg
     5701 atcaactaca ccatgctcaa tattggctgg accgtcggcc cgccactcgg cacgctgttg
     5761 gtcatgcaga gcatcaatct gcccttttgg ctggccgcta tctgctctgc gtttcccatg
     5821 tttttcattc aaatttgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
     5881 gtctggtcgc cgaaagtttt attacaagat aaggcactgt tgtggtttac ctgctctggc
     5941 tttctggctt cttttgttag tggcgcattt gcttcatgca tttcacaata tgtgatggtg
     6001 attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgccgcc
     6061 atggtagtta cgttgcaata ttccgtgggc cgccgactta acccggctaa catccgcgcg
     6121 ctgatgacag caggcaccct ctgtttcgtc atcggtctgg tcggttttat tttttccgga
     6181 aacagcctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aataatttat
     6241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
     6301 ttttccgccc agtctttagg ctggcttggt gccgcaatta acccattagt gagtggccta
     6361 gtgctaacca acctgccgcc ttcctcgctg tttatcatct tagcgttggt gattattgct
     6421 gcgtgggtac tgatgttaaa agggattcgt gcaagaccgt ggaggcagcc cgcgctttgt
     6481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta cctctgctaa
     6541 tatgtttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
     6601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
     6661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
     6721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
     6781 aaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt gtgttttgct
     6841 tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt agcatcagtt
     6901 ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt cgtcaataaa
     6961 catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg ctcttccaat
     7021 gaccacatcc agaggctctt caggaaatgc gcgactgaca cctgctgtca cggtaatgtt
     7081 gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa ttctgacacc
     7141 tgcacgacat gcttcttcat cattagccgc tttgacaata atgataaatt cttcgccccc
     7201 gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg ccagggtgcg
     7261 taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa atcggtcaat
     7321 atccaacaac attaaataaa gattcagagg ctcagtgttg cgtaactgat gatcaaagga
     7381 ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc tacggattgt
     7441 cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagtaacc cttcctgaaa
     7501 agcggcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta attcccgacc
     7561 acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta attcatcgtt
     7621 atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat aagaatggtt
     7681 atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca tactcaccag
     7741 ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat caatttccgt
     7801 tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta ttctaacgag
     7861 agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt catatctgag
     7921 ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc tgtaaattac
     7981 ctctgcatta tcgtaaataa aaggatgaca aaaagcataa cccaaaccct aatggcccag
     8041 tagttcaggc catcgggcta atttattttt atttctgcaa atgagtgacc cgaacgacgg
     8101 ccggcgcgct tttcttatcc agactgccgc taatgttgat catctggtcc ggctgaactt
     8161 ctcgtccatc aaaaacggcc gcaggaataa caacattaat ttcaccgctc ttatcgcgaa
     8221 aaacgtaacg gtcctctcct ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc
     8281 catcgtgcat ggtttttgcg aaatcaacgg tcattttttt tgcatcatcg gttccgcgat
     8341 agccatcttc tattgcatga ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt
     8401 catctgccaa cgcataaggc atgacaagaa aacttgctaa tacaatggcc tgaaatttca
     8461 tactaactcc ttaattgcgt ttggtttgac ttattaagtc tggttgctat ttttataatt
     8521 gccaaataag aatactgcca attgttataa ggcatttaaa atcagccaac tagctgtcaa
     8581 atattcagag aatttaactc actaaagtta agaagattga agagtcttaa acatattttc
     8641 agaataatcg tatttatatg tttgaaaatt attatattgg acgagcatac agaaaaagca
     8701 aatcaccttt acatataata gcgtggacaa aaaacagtga acattaataa agataaaatt
     8761 gtacaactcg cagataccga cactattgaa aacctgacat ccgcgttgag tcaaagactt
     8821 atcgcggatc aattacgctt aactaccgcc gaatcatgca ccggcggtaa gttggctagc
     8881 gccctgtgtg cagctgaaga tacacccaaa ttttacggtg caggctttgt tactttcacc
     8941 gatcaggcaa agatgaaaat cctcagcgta agccagcaat ctcttgaacg atattctgcg
     9001 gtgagtgaga aagtggcagc agaaatggca accggtgcca tagagcgtgc agatgctgat
     9061 gtcagtattg ccattaccgg ctacggcgga ccggagggcg gtgaagatgg tacgccagcg
     9121 ggcaccgtct ggttcgcgtg gcatattaaa ggccagaact acactgcggt tatgcatttt
     9181 gctggcgact gcgaaacggt attagcttta gcggtgagat ttgccctcgc ccagctgctg
     9241 caattactgc tataaccagg ctggcctggc gatatctcca ggacagccat tggtggtgtt
     9301 tatatgttca agccacgatg ttgcagcatc ggcataatct gaggagcctt accgcgccat
     9361 tgtcgataca ggcgttccag atcttcgctg ttacctctgg aaaggatcgc ctcgcgaaaa
     9421 cgcagcccat tttcacgcgt taatccgccc tgctcaacaa accactgata accatcatcg
     9481 gccaacattt gcgtccacag ataagcgtaa taacctgcgg catatccgcc accaaaaata
     9541 tgggcgaaat aactgctgcg atagcgtggc ggtatagcag gaagatccat attttccgcc
     9601 accagcgccc gcaattcgaa atcatcgaca tcctgcattg cttcgttttc ttccaggcaa
     9661 tgccagcgca tatcgagaag tgcggcgcta agcagttcgc tcatctcata ccctttgttg
     9721 aacaggctgg cattacgcat tttctgttgc agttcgtcag gcattgctgc cccgctctga
     9781 taatgccggg cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc gttgatttgc
     9841 gacggaaatt cgacaaaatc acgcggcgtg ttggtaccgg agagcgtggc ataacgctgg
     9901 cgggcaaaga ggccgtgtag cgtatgacca aattcatgga ataaggttat gacttcatcc
     9961 cagagtaaca acgcaggctc accggcagcg ggtttctgat aattgcagac gttataaatt
    10021 accggatgcg tttcattaag cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg
    10081 ctttttgaat cacgggcgaa gaaatcaccg taaaataacg ccagccccac gccattatga
    10141 tcaaaaattt cccacacccg aacgtcagga tggtagacag gaatatcaaa acgttcgaca
    10201 aacttaatac cgaagagctg attcgcggtc cagaatacac cttcattcaa caccgtgttt
    10261 aattcaaaat atggcttgag ctgcgcctca tcaagatcaa atttctcccg ccggacctgt
    10321 tcggcataaa atccccagtc ccacggctgc gcgctaaacc cgccctgctg cttatcgata
    10381 accgcctgta tggaggctaa ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc
    10441 cgcataaagt taagtgctgc ttcaggtgtt tttgccatct gatcggcgat tttccatgcg
    10501 gcataatgag gaaaaccgag taatgtagcc tgttgtgcac ggatctccac cagacgttga
    10561 atgatagcgc gggtatcatt ggcatcattt ttttccgctc gcgtccagcc cgcaataaac
    10621 agtttttcac gcgtcgcacg atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc
    10681 agcagcggaa tcagccattt gttatccaga cctttctcgc gagccgcctc tgccgccagc
    10741 gcaatctctt gctcactcat tcctgccagc tgcgcgatat cgttcacaac cagactgccg
    10801 gatttatttg ctgccagtaa ccgctggtta aactgactgg tcagtgtcgc agcttctgta
    10861 ttcagtactt ttaattttgc tttatcagct tgtgcaagtt tggctccggc taggacaaaa
    10921 cgttgatgaa tcacctccac caggcggatg gattcactat caagccccag agattcacgg
    10981 cgttgccaga cagcatctac ccgcgcgaat aattcaccgt tcagatagat atcattagcc
    11041 agttccgcca gttcagcgga aaactgctcg tcaagacgct gtaattcatc attggtatgc
    11101 gccgcagtca tggcaaaaaa gacgctggta acgcgggtaa gtaattctcc gctttgttcc
    11161 agtgccagaa tagtattgtt gaaatcaggt gtttgcgggt taagcgcgat ggcagcaatt
    11221 tctgcccgct tttgcagcat tccctcatcg aatgccgggc gatagtgatg attggcaatt
    11281 tgatcaaaat ggggagccag atacggcagt gtgctttgca caaggaaagg attcattgtt
    11341 gtcattttct tctcctgaac gcgaggtgtt ccatagcgta ggcttactga taacggagtg
    11401 caatcttgca aaccagtatt acccgctctt aagcatcccg tgctatgtta ttgacacaca
    11461 aaagcgttga ggaacagtga gatgatcgtt ttagtaactg gagcaacggc aggttttggt
    11521 gaatgcatta ctcgtcgttt tattcaacaa gggcataaag ttatcgccac tggccgtcgc
    11581 caggaacggt tgcaggagtt aaaagacgaa ctgggagata atctgtatat cgcccaactg
    11641 gacgttcgca accgtgccgc tattgaagag atgctggcat cgctccctgc cgagtggagc
    11701 aatattgata tcctggtaaa taatgccggc ctggcgttgg gcatggagcc tgcgcataaa
    11761 gccagcgttg aagactggga aacgatgatt gataccaaca ataaaggcct ggtatatatg
    11821 acgcgcgccg tcttaccggg tatggttgaa cgtaatcatg gtcatattat taacattggc
    11881 tcaacggcag gtagctggcc gtatgccggt ggtaacgttt acggtgcgac gaaagcgttt
    11941 gttcgtcagt ttagcctgaa tctgcgtacg gatctgcatg gtacggcggt gcgcgtcacc
    12001 gacatcgaac cgggtctggt gggtggcacc gagttttcca atgtccgctt taaaggcgat
    12061 gacggtaaag cggaaaaaac ctatcaaaat accgttgcat tgacgccaga ggatgtcagc
    12121 gaagccgtct ggtgggtgtc aacgctgcct gctcacgtca atatcaatac cctggaaatg
    12181 atgccggtta cccaaagcta tgccggactg aatgtccacc gtcagtaatt tttatacccg
    12241 gcgtaactgc cgggttattg cttgtcacaa aaaagtggta gactcatgca gttaactcac
    12301 tcacaagcaa gaacgaatga ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca
    12361 gcagatttat cgtatccttc gtcgcgacat tgtccattgc ctgattgccc caggcacacc
    12421 gttgtcggaa aaagaagttt ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc
    12481 ctttattaaa ctggcggaaa acggcctgat tcaaattcgt ccgcagcgtg gcagctacgt
    12541 caacaaaatt tccatggccc aggtgcgcaa cggcagtttt atccgtcagg ccattgagtg
    12601 cgcggtggcg cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc aactggaaca
    12661 aaatcttcac cagcaacgca ttgccattga gcgcaagcaa ctggatgatt tttttgaact
    12721 tgatgacaac ttccatcaac tcctgacgca gattgccgac tgtcaactgg cgtgggatac
    12781 cattgagaac ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg accacgtttc
    12841 tccaccagaa atgctgttac gccagcatct tgatattttc tctgccctgc aaaaacgtga
    12901 tggcgatgcg gtagaacgtg caatgacgca acatttgcag gaaatcagcg aatccgtgcg
    12961 ccaaatccgc caggaaaaca gcgactggtt tagcgaagag taattcattt cctctcatcc
    13021 catccggggt gagagtcttt tcccccgcct tatggctcat gcatgcatca aaaaagatgt
    13081 gagcttgatc aaaaacaaaa aatatttcac tcgacaggag tatttatatt gcgcccgtta
    13141 cgtgggcttc gactgtaaat cagaaaggag aaaacaccta tgacgaccta cgatcgtaac
    13201 cgtaacgcaa tcaccactgg cagccgtgtt atggttagcg gcaccggtca cactggcaag
    13261 atcctgtcga ttgatactga aggtctgacc gctgagcaaa tccgccgcgg aaaaaccgta
    13321 gttgttgaag gttgtgaaga gaaactggca ccactggacc tgattcgtct cggcatgaac
    13381 taagcgtgtg aatgccgccg atggcggcat tgctttttta cttcacggaa tatttcgcca
    13441 cggtcgcttt cgcaccatgc gctaataaaa acaagtacgc ttccgtcact tttgcagtaa
    13501 acaagctatt gtctggcaaa tcatcaccaa agatcgcctt aatcgccagc aatgactgga
    13561 cgcgcgcttt cccttcggca ctactttgta cagccttctg aataacaggt aacagtgggt
    13621 cactgatttc aatcggattt ccctgttcat caacaccacc gacataacgc atccaacccg
    13681 cgacgcccag cgccagcaga tcgaacttgc tgtcatgcgc cagatgccag cgaacagaat
    13741 ccaacatccg ctgtggcaat ttctggctgc catccatcgc aatctgccag gttcgatggc
    13801 gtaacgccgg gttgctatag cgtgcaatta atcggttagc gtaatcttgc aaatcaacgc
    13861 cctgcacttt cagcgtcggc gcttgttcct gcaacatcaa ggcatacgcc gcataacgat
    13921 aatgttcatc ttccatacag tcattaatgt gctgatatcc ggcaagataa cccaaatacg
    13981 ccaggaatga atgactgccg ttgagcatgc gcaacttcat ctcttcataa ggcagcacat
    14041 cactaaccag ttcggctccc gctttttccc attccggacg tccggcaaca aagttatctt
    14101 cgattaccca ctggcggaaa ggttcacagg caacgccagc aggatcgcgc acaccggtaa
    14161 gctgttcgat tttcgctagc gtatcctctg tcactgcggg cacaatacgg tccaccattg
    14221 ttgatgggaa agtcacgtta tcttcgatcc attgtgccag ttttacatca acggcttgtg
    14281 cgtatgaagt gacaacgtca cgcataacat gaccgttttc tggcatgttg tcacatgaca
    14341 tgacggtaaa tgcgggaagt cctgccgctt tacggcgagc cagcgcctca acaatcaccc
    14401 ctgttgctgt tttcggctgg tggggatttt gcacgtcggc agcgaccatc gggtgatcga
    14461 gcattaactg tccggtcgcc ggagagtgga aatacccttt ttcggtgatt gtcagagaga
    14521 caatcgcgat ttgcggttca cacatcgcag ccaacacggt ttctaagcca tccatctgta
    14581 cgtgcaaggc ttttttaacg acgccaacga cgcgtgccgt ccacgcatcg gccgacattt
    14641 ccgcaacggt ataaagatta tcttgctgtt gtaaatcggc aatttgctgt tcgccaccga
    14701 ttaagttgac ctcataatat ccccagtcac tgaaatgttc tgtagcaaga atatcggcat
    14761 acacgccctg atgcgcacgg tgaaatgcac caaagcctaa atgaacaatt cttggggcca
    14821 agttattacg atcataaaca gggagcgtcg cttttgctga taacaaatta tttcccataa
    14881 ctattcctta aatataaata tggcaagcta catgttttgt tatatgaata aaaaatcccc
    14941 tctccggtaa gagaagggat tgaggattta aagacttctg gaaggttgcg cagctcttac
    15001 aacacgcggt tgatcttccg cagcgtcttc cagcgcactt aaatcacggt ctttcacttc
    15061 tggcattttc agcgcagaga ttaaaccaat cactgaatat gccatgatca taatggcgat
    15121 cggataccag gattccgtca tggtgcagaa aatacccgca aggataggac caaaaccgga
    15181 agcgataaga ccaccaattt ctttagaaat agccatccgg gtaaagcggt ttttacagcc
    15241 gaacatttct gccatggtaa tgttttccag agcaaataat cccagcaccg cacagttatg
    15301 aatcacaatc agtgcaacca taatggtgct cggggcatag cttttatcta cgatgataga
    15361 aagcattggc catgccagca caatcgcaga ggtattcata ataatatacg ggatccggcg
    15421 accaatttta tcggataacc aaccaaggaa cggaatggtc ataaagccga gaatcgaact
    15481 gatcatcaat gcatctgttg gaattgcttt gttaaacaat aacgtctgta ctaaatagcc
    15541 tgcaaggaaa gtctgaatta acccggagtt acctgcctga ccaaaacgca gccctgttgc
    15601 cagccagaag gatttgctct ggaacatgct accagcaggt gcaggttttg ctgtcggttg
    15661 gttgctgtcg ttaaccttct caaagaccgg gctttctttc agattcatac gcaaccagat
    15721 agcaaagacc atcacgacaa cgctcgccag gaacggtata cgccatcccc acgccagcag
    15781 ttcctcttta ctgagaatga agaacataaa ggcccagatt gccgttgcgc tcaaggttcc
    15841 gcagttagtt cccatagcca caaatgagga gataattccg cgcttacctt ttggcgcata
    15901 ttccgccagc atcgtaccgg caccggaaat ttccgcacct gcacccaatc cctgaataat
    15961 acgcagcgtc accagcaaga tgggggcaaa aacaccaatc tgtgcatagg tcggtaacac
    16021 tccaattaag gtggtacaga tccccatcat ggtgatggta ataaagagca cttttttacg
    16081 ccctattctg tcgcccattt tgccgaaaat aaatgctccg acaatacgcg ccacataacc
    16141 tgcaccgtag gttcccattg ccagaattaa cgccattgcc gttgatgatt caggaaaaaa
    16201 tatttcatga aacactaacg ctgcgccgag cgaatataac tggaaatcca taaattcaag
    16261 cgcagtgccc agccatcccg atactgctgc tttcaccaaa tccttagtgc ttctttcgtg
    16321 tttttctatt gtcataatgg ttatctctaa aaaagaggta agatgcgtac tacttactcg
    16381 ccgttattgg tattattcag aaaaagtgag taagactttg cagcaatgtt tttgatcctg
    16441 ttcaaataaa ctaatggcat cagcaacatg ctggaaatca aacgtatggg taattaattt
    16501 ttctggttta attaaccctt tacttaacca gtcgataacg accggaaatt tatttgcatt
    16561 taagcgtgaa gagaaaatag agagttcttt tccggtaatt ccttgctgaa tcacttcaga
    16621 aggttcactg gagaagccca tcaatacaat acgtgccgct ggagaagcca gcgttacggc
    16681 ctctttcagg atagaaggat gacaagccgc atcgataatt aatgtcggct ttatgccttt
    16741 ttcagcgaaa atctcgccaa gcggtgtctg gctgttatta atcacccaat cagccccgct
    16801 ctctttcgct ttttccagtc gttcatcaat gcgatcggca acaatcacat ttttaacgtt
    16861 atagacgcct tttaatacct gaacgatcgt caggccgatt ggaccggcac cataaaccag
    16921 aacggtatca ttttcagtcg gttgaccatg tccggttacg ttagccgcaa tggtaaaagg
    16981 ttcgatcatc accgcatatt gatcggccac tgcttcagga attttccacg cattttttgc
    17041 cggaaccaca gcatattcac tgaaaccacc gtcaacatgc acacctaata cagcgagtgt
    17101 cgtacaaacg ttcggcttac ctatagagca cggatagcaa tgaccacagc tgaccaccgg
    17161 atcgacagca acacgttcac cgactctggc gctttccaca ccttcaccca ccgcatcaat
    17221 gacgccaaag aattcatgtc caatgacgcg cggatatttc gcaaaaggat tatgcccacg
    17281 ataaatatgg ctatctgaac cacaaattcc ggcaagtttt acttttactc gaacttcacc
    17341 cgctgacggg gtgggtattt cacgttcgat aatcgccagt tgattcggtt tttcaattaa
    17401 aatgcttttc attaacttac tccttaccag ttccacagcg tgccatcttc cagacgtgcg
    17461 actggtagat aagcaggttc atagggatat ttcgccgcca gcttttcatc gaattcgatg
    17521 ccaagacccg gtttgtctcc cggatgcata tagccgttat cgaaagtcca gttgtgcggg
    17581 aagacttcga gcatttgttc ggaataaccc atgtattcct ggacaccgaa attggggacc
    17641 cacagatcaa agtgcagcgc cgcagccatg cagactggtg acaaatcgga aggaccgtgt
    17701 gagccagtac gtacctgata caacgaagca aaatcggcaa tccggcgcat accggtaatt
    17761 ccgcctgcat gggtcagcgt ggtgcggata taatcgataa gttgctcttc aatcagttgc
    17821 ttgcagtccc agatgctgtt gaagacttca cccaccgcga tgggtgtgat ggtatgttgg
    17881 cgaatgagac ggaagcattc ctggttttcc gcaggcgtcg ggtcttccat ccagaacatg
    17941 cgataatctt caatgctttt accaaagcgc gccgcttcaa taggcgttaa gcgatggtgc
    18001 atgtcatgca gcaaatgttc attaaaacca aacttgttac gtaccgcgtc aaacaatttc
    18061 ggcatgaaat cgaggtattt ctccgtcgac cacagctgct cttccggcca ctgtcctttg
    18121 gttgcgggtt cataagccag acctttacct ttcgacatgc cgtaggtggt tttcatacca
    18181 gggatgccgc actgcacgcg gatggctttg aatcccagct cctgatgacg ggcataatca
    18241 tccagagctt catcaatact gtgaccggtg gtatggcaat aaaccatcac cccttcacga
    18301 gacgcgccgc cgagtaactg gtaaagcggc atgttggcag ctttggcttt aatatcccac
    18361 agcgccatat caaccgctga aatggccgac atcgtaaccg gaccgcgacg ccagtaagca
    18421 cctttataga aaaactgcca gatatcttcg atacggtgcg catcgcgacc aataagctgc
    18481 ggacaaaggt gatcctgcaa ataagaggcc acagaaagct cacgtccatt gagggtggca
    18541 tccccaaggc ccgtaatacc gtcctcagtg gtgattttta atgtgacgaa attacgcccc
    18601 ggacaggtaa caaaaacttc agcctttacg atcttcatgt tcgattcctt gcatcgcttg
    18661 tcgtgatgca tgaaatctac gcaactgagc tactaccata caagtatgaa gatcaaaaaa
    18721 agccggagtg atcacaaaaa aaggcgtata ttttcgccat gaatggttga caaaagatga
    18781 aatagagtac cttttatcag ctgacgcttc ctcgtatctt attgataaaa tagatttatg
    18841 tccttacgtg cgcccccagc cggcaacaat gatcaacatg ccgcaaagcg caatcaacgc
    18901 acccgtccag tcataaagag tcagtttcac gccatccaca acgcgcagcc acatcaacgc
    18961 cgtgcagaca taaacgccac cataagccgc gtaaacacgc ccactcgccg caggatgcaa
    19021 cgttaacaac cagacaaaca gcgccagtga aatccccgcc ggaagcaaca gccagatact
    19081 ggcgtttcgt tttaaccaca accagggcag aaagcatcca ataatttcac acagcgcagt
    19141 agcaaaaaat agtaacgttg ttttaatcat ctttgtcact tattgacatc atgtatagtt
    19201 atagggcgac ataatatcat caatataaac accctcctgg tacgttttgc gttcgcagtg
    19261 gatggtgtag aatcaccttt aatcattcat acagggaatg aattatgaaa atcactctca
    19321 gcaaacgaat cggcctgctc gcttttctgc tgccttgcac actggcattg agcacaactg
    19381 ttcatgccga aactaacaaa ctggtgattg agtctggcga cagtgcacaa agccgccagc
    19441 acgccgctat ggaaaaagag caatggaatg acacgcgcaa tctgcgtcag aaagtgaata
    19501 aacgcactga aaaagagtgg gataaagccg acgccgcttt tgataaccgc gataaatgtg
    19561 agcaaagcgc caacatcaat gcctactggg agccaaatac tttgcgctgc ctggaccgtc
    19621 gaactggccg cgttattacc ccctaacctg ttattgattt aaggaatgta aggacacgtt
    19681 atgccaagcg cccacagtgt taagctacgc ccgctggagc gtgaagattt acgctatgta
    19741 catcaactcg acaataacgc cagtgtgatg cgttactggt ttgaggaacc ctacgaagcc
    19801 tttgttgaac tctctgatct gtatgataag catattcacg atcagagcga acgacgcttt
    19861 gtggtggaat gtgacggcga aaaagccggt ctggtggagt tggtggaaat taaccatgta
    19921 catcgccgcg cagaatttca gataattatc tccccggagt atcaggggaa aggtctggca
    19981 acccgtgccg ccaaattagc aatggactat ggcttcaccg ttctcaatct ctataagctg
    20041 tatctgatcg ttgataaaga gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt
    20101 acggttgaag gtgaattgat gcatgagttc tttattaatg gtcaatatcg taatgccatt
    20161 cgcatgtgta tattccagca tcagtatctg gcagagcaca aaacaccggg tcagactctc
    20221 ctgaagccga ccgcacaata gcattaataa taatcgatcg tatttttgat ggtgtaaacc
    20281 cgttcgacgg cgggttttac tccttcatca acaatgatca gctgacagtc caccggattg
    20341 gcgtgactgt catattcaca gctctgtttt acattaccaa ctcgttgatt attcagtaaa
    20401 gtaaccgctg tgtaatctaa ttttttgatc gggtccgttg atggcgtggc gctgacagat
    20461 aatgttttgt cgttactttt cgtggtttta cccagtggat aaccctgatc atcatagcga
    20521 tattccatct gcatttgttt gctgctggct ttaatcacga agccattatc atcggtttcc
    20581 caactcaccc cggcagaagg taattctgct agctggcatt ttccctgtaa acgtactctc
    20641 ttctccagcg tctcggcatc acggtaataa ttggcgtcca gtaccagagc gaccacggta
    20701 ttattttcca gatccagtaa ttcgagtgaa tcaaaacagc cttcttccga caaagtcccc
    20761 gaaacacgtt tcgtcacttc accttgctca tccattaatg tctgagtgaa atcttttacc
    20821 ggaccacgca gcggatcaaa atcgaattca ttagagaaac tggccatctc aggagtaaat
    20881 gaaagtgtta cctctgtgcg gtcacatcct gtgagtaata tcgcgagtaa gcatggcagt
    20941 aatttgtatt tcacatcagt caccagagag tagagatgat tctcaatcat agtagcaaat
    21001 acagtacttt acacgttaaa tgctatgctt aaagaagtta tcttcgcgta aggagcttat
    21061 gatgaaactc tcaacctgct gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact
    21121 cgccgcgccg ggatcctgtg agcgcataca aagcgatata tcacagcgca ttatcaataa
    21181 tggcgtaccc gaaagcagct tcacgttaag tattgtaccc aatgaccagg ttgatcagcc
    21241 tgattcccag gtcgtcgggc attgcgctaa tgatacgcat aaaattctct acacccgcac
    21301 caccagcggt aacgtctctg ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc
    21361 gcagtaatac attatcgact gaacgccgga tacgacgcat ccggcgattt gaacatataa
    21421 cataacccac cttattactc atacccttct attgatatgg attaataatt cttaacccaa
    21481 aatgggtaga ctccctctat tgttagcgcg ctaaatattc aatatataaa cttttatata
    21541 acgataaaga acagggagtg agttatgtcc aaaaatgaac aaatggtggg catcagccgc
    21601 agaacactcg ttaaatctac cgcgataggt tctctggcgc tggctgcagg cggtttttct
    21661 ttgccgttca ccctgcgcag tgcagcggca gcagtacaac aggccagcga acaagtggtc
    21721 tggggtgcct gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat
    21781 aatgaagtgg cctgggtgga aactgacaac accggcagcg atgagtatgg caaccatcag
    21841 gtacgcgcct gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg
    21901 aattacccaa tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg
    21961 gatgaagccc tggatactat cgccagtagc ctgaagaaaa ccgtcgaaca atatggcaat
    22021 gaggctgtat atattcagta ctcttcgggg atcgttggcg gcaatatgac ccgctcttcg
    22081 ccatcagcct cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat
    22141 ggctcctaca gcaccgcaca aatttcctgt gccatgccct acacctacgg cagtaatgat
    22201 ggcaacagca ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg
    22261 gcagaaaccc gcatgagcgg cggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa
    22321 tcgaacgcca aaatgattgt tatcgatccg cgatataccg atacggctgc tggtcgtgag
    22381 gatgaatggc ttcctattcg cccgggcacc gatgccgcgc tggttgcggg gattgcctgg
    22441 gtattgatta acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat
    22501 gacgaaaaaa ccttacccgc agatgcacca aaaaatggtc actataaagc ctatattctt
    22561 ggtgaaggtg atgataacac agcgaaaacg ccgcagtggg cttcgcaaat taccggtatc
    22621 ccggtggacc gtatcatcaa actggcgcgt gaaattggca cagcaaaacc cgcttatatc
    22681 tgccagggct gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct
    22741 atgttgccta ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa
    22801 tcgacctata ccattaccat agagcgcctg ccggtgctgg ataatccggt caaaacgtca
    22861 atctcctgct tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcaatccgc
    22921 gacggcgtcc gcggcaaaga taaactggat gtgcccatta agttcatctg gaactacgcg
    22981 ggaaataccc tggttaatca gcattctgac atcaacaaaa cgcatgaaat tctgcaagac
    23041 gaatcgaaat gcgaaatgat tgtggtcatc gaaaacttta tgacctcatc ggcaaaatat
    23101 gccgacattc tgctgccaga cctgatgacc gttgagcagg aagatattat tcctaacgac
    23161 tacgccggta acatgggata tctcattttc ctccagcctg tcaccagcga aaaattcgaa
    23221 cgcaaaccga tttactggat cctgagtgaa gtcgcgaaac gtcttggacc tgacgtctat
    23281 caaaagttca cagaaggtcg cacgcaggaa caatggttac aacatctgta cgccaaaatg
    23341 cttgccaaag atccggcgtt accgtcttac gacgaactga aaaaaatggg tatctataag
    23401 cgtaaagatc ccaatggtca ttttgtcgcc tacaaagcat ttcgtgacga ccccgaggca
    23461 aatccactta aaacaccttc cggtaagatt gaaatttatt ccagcaagct ggcggaaatt
    23521 gcccgtacct gggaactgga aaaagatgaa gtgataagcc cactgccagt ttatgcctct
    23581 acctttgaag gctgggactc acctgaacgt agaaccttcc cactgcaact gtttggtttc
    23641 cattacaaat cccgtactca ctcgacctac ggcaatattg atctcctgaa ggctgcctgc
    23701 cgtcaggagg tgtggatcaa ccctatagat gcgcagaaac gtgggattgc caacggcgat
    23761 atggtgcggg tgtttaacca tcgtggcgaa gttcggctac cagccaaagt gacaccacgc
    23821 attctccctg gggttagcgc tatgggccag ggagcctggc acgaggccaa tatgtctggc
    23881 gataaaatcg accatggagg ctgtgtgaat acgctaacca ctctgcgccc ttcaccactg
    23941 gcgaagggaa acccgcagca tacgaatctg gtcgagatcg aaaaaatata acccactaca
    24001 accataaatt ctggcatgac attttgtttg aaaagcaata agtgagtaat gatgaaaatc
    24061 cataccacag aggcgctcat gaaggctgaa atcagccgta gaagtctgat gaaaacctcc
    24121 gcacttggca gtcttgcgct ggcaagcagt gctttcactc tgccattttc ccaaatggtc
    24181 cgggcggcag aggctccggt agaagagaaa gcggtctgga gttcctgcac cgttaactgc
    24241 gggagccgct gtctgttacg tttgcatgtg aaagatgaca ccgtgtactg ggtggagtct
    24301 gatacgacag gtgacgacgt ctacggtaat catcaggttc gagcgtgttt acgcgggcgc
    24361 tctatccgcc gacggatgaa tcatcctgat aggttgaaat atcccatgaa gcgcgtcggc
    24421 aagcgcggtg aaggtaaatt tgaacggata agttgggacg aagccctgga taccatcagt
    24481 gataatcttc ggcggatcct gaaagattac ggcaatgagg ctgtacatgt cctgtacgga
    24541 acaggtgtag atggcggaaa catcaccaac tcaaacgtcc cgtaccgtct gatgaactct
    24601 tgcggtggct ttctcagtcg ctatggtagc tacagtaccg cacagatcag tgccgcaatg
    24661 agttatatgt tcggtgccaa tgatggcaac agcccggatg atatcgccaa tacgaaactg
    24721 gtcgttatgt tcggaaataa cccagcagaa acgcggatga gcggcggtgg tgtcacttac
    24781 tacgtcgagc aagcccgcga acgttcaaac gcacgcatga tcgtcatcga tccacgttat
    24841 aacgacactg ctgccgggcg tgaagatgaa tggctgccca ttcgccctgg caccgatggc
    24901 gcactggcct gtgcgattgc ctgggtactg attactgaaa acatggtcga tcagccattt
    24961 ctcgacaaat attgtgttgg ttacgatgaa aaaacgctgc ccgccaacgc accacgtaac
    25021 gcgcattata aagcctatat tctgggcgaa gggcctgacg gcatagctaa aacgccggaa
    25081 tgggcagcaa aaatcaccag catcccggca gaaaaaatta tccagttggc acgagagatc
    25141 ggttcagcaa aacctgctta tatttgtcag ggttgggggc cacaacgaca ctccaacggc
    25201 gaacaaacat cccgcgctat tgccatgctt tccgttctca ccggtaacgt cggcataaac
    25261 ggcggcaact caggcgtacg cgaaggtagc tgggatctgg gggtagaatg gttcccgatg
    25321 ctcgagaatc ctgttaaaac gcagatttcc gtctttacat ggacagatgc catcgaccat
    25381 ggtacggaaa tgaccgcgac ccgtgatggt gttcgtggaa aagaaaaact ggatgtcccc
    25441 atcaagtttt tatggtgcta cgccagtaac acattgatca atcaacatgg cgacatcaat
    25501 cacacccatg aggtgcttca ggatgacagc aagtgcgaga tgattgttgg cattgaccac
    25561 ttcatgacgg cctcggctaa gtattgcgat atcctgttgc ccgacctgat gccaacagag
    25621 caagaagacc ttatctctca tgaatctgca gggaatatgg gctatgtgat cctcgcccaa
    25681 cccgcaacct cagcaaaatt tgaacgcaaa cccatctact ggatgctgag tgaagtcgcc
    25741 aaacgcttag gaccggacgt ttatcaaacc tttactgaag gtcgcagtca gcatgaatgg
    25801 atcaaatatc tccatgcgaa aacgaaggaa cgtaacccta agatgcccga ctacgaggag
    25861 atgaaaacga ccgggatctt taagaaaaaa tgcccggaag aacactacgt cgctttccgc
    25921 gcattccgtg aagatccaca ggcaaacccg ttgaaaacac cttcggggaa aatcgaaatt
    25981 tattctgaac gactggcgaa gattgcagat acctgggaat tgaaaaaaga tgaaattatt
    26041 catccccttc ctgcgtatac cccaggtttt gatggctggg acgatcccct gcggaaaacc
    26101 tatccactgc agttaacggg cttccactac aaagcgcgta cccactccag ctacggcaat
    26161 attgatgtgt tacagcaggc ctgcccacaa gaggtgtgga tcaaccccat tgatgctcag
    26221 gctcgcggta tccgtcatgg cgataccgtg cgggtattta acaataatgg agaaatgctg
    26281 attgccgcaa aagtgactcc gcgtattctg cctggcgtta ccgccatcgg gcaaggtgcg
    26341 tggcttaaag cggatatgtt tggtgaccgg gtcgatcacg gcggcagtat taatattctg
    26401 acctctcacc gcccttcacc gctggcaaag ggaaacccgt cgcacagcaa tcttgtccag
    26461 atcgaaaagg tttaaggagt agcccatgac cacacaatat ggatttttta tcgattccag
    26521 ccgctgtact ggctgtaaaa cctgcgaact ggcgtgcaaa gattttaaag atcttggccc
    26581 ggaagtcagt ttccgccgta tttatgaata tgctggcggc gactggcagg aagataatgg
    26641 cgtctggcac cagaacgtgt tcgcttatta tctctccatc tcctgtaacc attgcgatga
    26701 ccccgcatgt acaaaagtct gccctagcgg cgcaatgcat aagcgtgaag atggctttgt
    26761 ggtggttgat gaagatgttt gtatcggctg ccgctactgc cacatggctt gtccgtacgg
    26821 cgcgccacag tacaatgctg aaaaagggca catgacgaag tgcgatggtt gttattcgcg
    26881 cgtcgccgag gggaaacaac ccatatgtgt cgaatcctgc ccgctgcgtg cacttgagtt
    26941 cggtccaatt gaagagctgc gccagaagca cggcactctg gctgccgtcg cgccactgcc
    27001 gcgcgcgcat ttcacaaaac ccaatatcgt tatcaaacct aacgccaaca gccgcccgac
    27061 cggcgatacc actggttatc tggctaatcc ggaggaagtg taaaatggga aatggatggc
    27121 atgaatggcc actggttatc tttaccgtat tgggtcaatg cgtagtaggc gcgctgattg
    27181 ttagcggaat tggctggttc gccgccaaaa atgacgcaga ccgtcagcac attgttcgtg
    27241 gcatgttttt cctctggctg ttgatgggca tcgggtttat tgcctctgtc atgcacctgg
    27301 gatcgccact gcgggcgttt aactcgctga atcgaatcgg agcttctggt ctaagtaacg
    27361 agattgctgc cggttccata ttttttgccg ttggcggact atggtggctg gtggcggtca
    27421 tcggtaaaat gccgcaagcg ttggggaaac tctggttact tgtcagcatg gcgctcggcg
    27481 tcattttcgt ctggatgatg acctgcgtgt atcaaatcga cactgtgcca acctggcata
    27541 acggttacac tacgctggct ttcttcctga cggtattgtt gagtggtcca atacttgctg
    27601 ccgcaattct gcgggcagcc cgcgttacat ttaataccac accatttgcc atcattagcg
    27661 ttctggcgct gatcgcttgt gccggggtga ttgtgctgca aggtttgtct ctgtcgtcta
    27721 ttcactcatc cgtgcaacaa gccagcgcac tggtaccaga ttacgcctcc ttacaggtct
    27781 ggcgtgtggt attgttatgc gcaggtctag gatgctggct ttgtccgctc attcgtcgcc
    27841 gtgaacctca cgttgccgga cttattctgg ggttaatcct cattcttggc ggtgagatga
    27901 tcgggcgtgt gctcttttat ggcctgcaca tgacggtagg aatggctatt gcaggttaac
    27961 aaattggtgc ggggcaaccc gcacatttca ggatgttagg aatgacccat ttttcacagc
    28021 aagataattt ttctgtcgtg gcgcgcgtgt tgggcgcgct gttttattac gctccagaga
    28081 gtgcggaagc cgcacctctg gttgcggtac tcacccgtga cggctgggaa actcagtggc
    28141 ctctaccaga agcgtcatta gcgccgctgg tgacggcttt tcagacacag tgtgaagaga
    28201 ctcacgccca ggcctggcag cgtttgtttg tcggcccgtg ggcactgcct tcaccgccgt
    28261 gggggtcggt ctggctggat cgcgaatctg tgctgtttgg cgattcaaca ttggcacttc
    28321 gtcagtggat gcgcgagaaa ggcattcagt ttgaaatgaa gcaaaacgaa ccggaagatc
    28381 attttggttc actgctgttg atggctgcgt ggttggcgga gaatggtcgc cagacagaat
    28441 gcgaagaatt gctggcatgg cacctttttc cgtggtcaac acgttttctt gacgttttta
    28501 tcgaaaaagc agaacaccct ttctaccgtg cactgggtaa actggctcgc ttaacgctgg
    28561 cgcaatggca atcacaactg ttaattcctg tcgcggttaa accgctgttt cgataggatc
    28621 accataatat tgccggatgg tgacgattac gcgcaattcg gcaataatac gttacagtgt
    28681 cacgtttttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc ttttgccgca
    28741 aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca catctatccg
    28801 aatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc
    28861 tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt cctcaataat
    28921 gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg gctgctaact
    28981 ccggcgctcg gcggactggc ggcgggtttg ttgctgatgg gctggcagaa atttacccag
    29041 caacgccctc atgcgccgac cgattatatg gaagcgttgc aaaccgatgg tcagtttgat
    29101 tacgcagcaa gcctggttaa atcgcttgcc tctctgctgg tagtaaccag cggcagtgca
    29161 attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg ttttgcccaa
    29221 cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc ggcgggaatg
    29281 gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt gctgtttggc
    29341 actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc attgctggtt
    29401 agcaatctga ttaatcatag cgacgcgtta ctctacagcg tacaactctc agtgacggtt
    29461 caggctcgtg actatgcgct gattatcagt acaggtgtgc tggcaggtct gtgcggacca
    29521 ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg
    29581 ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt cacacctgca
    29641 gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccgcc actgttaatg
    29701 atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc
    29761 gcacccggtg gtgtctttac accgacgcta tttatcggtc ttgccattgg catgttgtat
    29821 ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacgctttt actcggattg
    29881 accgggatgg cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg
    29941 atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc ctgcgtaatt
    30001 gcgtcggtaa tctcgcggac gttacaccgt gactctatct accgccagca cactgcgcag
    30061 catagctaag cggatgtatt gccccagttc acgctgttcg gcgcgcggca gataaggcag
    30121 ttcaccaatg agcggtgccg gaagtttttt accgagcaca tcaatgattt ccgcataatg
    30181 cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc cgtcgttggc
    30241 aatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac cgacaaccat
    30301 caacaccggc agttgttcct gcaccaccca ttcagagagt ggacgcaaat cattcatcag
    30361 actgcgccag ccgccagtcc cttctaccac gacatgatcg actttatcgg tcaggtttgc
    30421 caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgctacgc tactttcttc
    30481 ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg aaacactctg
    30541 caacaccagg gcatctttat tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac
    30601 gggtttatat cccgcaaccg tttttccctg ggaggctaac gcttgtagca atgcgcggga
    30661 aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct tcagcatcac
    30721 taactccacc gttatgcttc acaaatataa accaggaaaa taattaacct tgaaagtcta
    30781 agttatgctt tcctggccca aattgagata gcgcaaattt tggtagaaca gttaaaaaat
    30841 gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct tttaccagcg
    30901 cagcccctgc catcgtgccc tggttagaaa actgagtact ctcaacgcta atgtgctgac
    30961 tatacgcagg aagggcctgc tgacggatgc tatctgagat gaccgggaag aggatatctg
    31021 ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa ttcaccatga
    31081 tggcaagaat gcgccctaca tgcgcgccca ccccggtaat gatgtctttt gccagcagat
    31141 cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgccca tgtaacatcg
    31201 agctcatgga ttgattgaga cgcagctgtg ccagctcaag aatactgtcc acactggcga
    31261 tggtttcgag gcagccgtgg ttcccgcaat aacagcgttt accatatggg tctacctgtg
    31321 tgtgacctat ttccacgagg ctactgctgc ctgcgtgtag cagatgacca tcggtaatga
    31381 cgcccgcccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg
    31441 caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata taaaccggaa
    31501 cgccggtatg ctgctccaga gcctctccga gcggcatctc ttttacatcc tcgtagaacg
    31561 gcatgcgatg tacaatgcca ttttccgtat caataattcc cggcagggtt atggcaatcg
    31621 aagttagacg ctcaagtttt ttctggtggc ggataaaaaa ctgatcgata tgggaaatga
    31681 tacgatccag caatggcgag tcatctttta acgccagttc ctgcgactct tccaccacca
    31741 gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg cgcagagaaa
    31801 gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca cggttccccg
    31861 cttctttgat ttccagctct tgcaccaggt gtgcttcgag catctcacgg acaattttag
    31921 tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag actggaccaa
    31981 gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga tcaatgtgcc
    32041 caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg aaataatctg
    32101 taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttaggc atgtgattaa
    32161 cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat tcagcagatg
    32221 aatacgaaag ttacgcgcag ctggactttg ttcatgatgt ttcggccaga ccaaccacat
    32281 ttcagaaacc gcatcctctt cagcaatcgg cacccagcgc atttcgttga gctgaacacg
    32341 tttaaatgac gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac cgatgatggt
    32401 cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc
    32461 gagaatatcg tcatacagcc ctgtcccgac gtgcggatca aaaaagacaa agggttcttt
    32521 cgccagttca gccagcgtta cattcgggtt atttgccagg ggatgatcgt gcgggatcat
    32581 cgccataagc ggttcatgga cgatgactgc gtgttcaagc gtctccggta acgctgtgtt
    32641 acgcagcaat cccatatcca gcgttccttc aatgagcgga gcgatttgct cgcgagtgtt
    32701 catttcgcgg gtttgtaaat ggacatcggg ataatctcgg cgaaacagcg ataacgtatc
    32761 ggacaccgcc cgaataaaag gagccgacga agtaaaacca atgcgcaact cccccgcttc
    32821 accctgatgc agtctttcag cgcgagcggc agcgtcatcc accatagaca ggatttgccg
    32881 actatctgca agaaactgtt ttcctgctgc cgtcagcaat acactgcgat tggttcgtgc
    32941 cagcagtcgg gcaccaattt gttgctccag cgcctgaatc tgctgactta gcggcggttg
    33001 cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa
    33061 gtaacgcaga tgacgaagtt caatattcat atttaaaaca tcttatttga gattattaat
    33121 atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc acaggcatat
    33181 ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg caagcgacac
    33241 tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc cgcaatttat
    33301 gcgcgtcacc ctggcgctgt tctctgccgg actggcaaca tttgcacttc tctattgtgt
    33361 gcagcctatc cttccggtgc tttcgcagga gtttggctta acgcccgcga acagtagtat
    33421 ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg gccccctatc
    33481 cgatgccatt ggtcgcaaac cagtgatggt cacggcgcta ctgttggcct ccatttgtac
    33541 gttactttcg acaatgatga ccagctggca cggcattttg attatgcgcg ccttgattgg
    33601 gctttcgtta agtggcgtgg cagctgttgg catgacttat cttagcgagg aaatccaccc
    33661 cagtttcgtg gccttttcga tggggttgta tatcagcggc aactcaattg gcggcatgag
    33721 cggacgctta attagtggtg tcttcacgga ctttttcaac tggcgaattg ctctggcggc
    33781 tatcggttgt ttcgcgctag cctcggcgtt gatgttctgg aaaatcctcc cggaatcacg
    33841 ccattttcgc ccgacctcgc tgcgacctaa gtcgttgttt atcaactttc gtttgcactg
    33901 gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt ttgctgatgg ggtcgttcgt
    33961 cacgctgttt aattacatcg gctatcggtt gatgctctcc ccctggcatg tcagtcaggc
    34021 cgtggttggc ttattatcgc tggcttattt gaccggtaca tggagctcac ccaaagccgg
    34081 aaccatgacc acccgctatg ggcgcggtcc ggtgatgttg ttttcgacgg gggttatgct
    34141 gtttggttta ctgatgacct tattcagctc gctgtggctg atctttgccg gaatgttact
    34201 cttctcagca ggattcttcg cagcccactc agtagccagc agctggatcg gcccccgcgc
    34261 aaaacgcgct aaaggccagg cctcctcgct gtatctgttc agttactatc tggggtcgag
    34321 tattgccggg acgctgggtg gtgttttctg gcataactat ggctggaacg gcgtcggcgc
    34381 atttattgct ctgatgctgg tcattgctct gctggtcggg acgcgtttgc atcgtcgtct
    34441 gcacgcctga aaaataagtc cggactgcgg taaatacccg tccggactta ttgcctgctc
    34501 aaaccaacgt taatagccat cctaaaatag acgaagcgcc agccaattcc cgcagcgcgt
    34561 ctagcgtcat caggattata agtacccaaa taaacggatt cattttgctg tgtgtcattt
    34621 attactgatg cgcagttatt ctaccgcttt gtaagtagta aaatagttaa cccgatcaag
    34681 actactatta ttggtagcta aatttccctt aagtcacaat acgttattat caacgctgta
    34741 atttattcag cgtttgtaca tatcgttaca cgctgaaacc aaccactcac ggaagtctgc
    34801 cattcccagg gatatagtta tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat
    34861 tgagggtatg acaatgaaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
    34921 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
    34981 agcagcgccg gcgaaaacta cacatcataa aaaacagcat aaagcagcac ctgcccagaa
    35041 agcgcaggcg gctaaaaagc atcataaaaa tgcgaaagct gaacagaaag cccctgaaca
    35101 aaaagcgcag gcagcgaaga aacacgccaa gaaacacagc catcagcaac cggcaaaacc
    35161 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
    35221 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
    35281 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
    35341 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
    35401 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
    35461 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
    35521 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccttgtt ttttggtcat
    35581 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
    35641 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
    35701 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcggt ggcgctgcgt
    35761 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
    35821 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
    35881 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacgccgttg
    35941 ccgttatttg agggagataa agccgcgctt accgccgcat taaaagcggc aggtcgtaaa
    36001 gtgactcagg caggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
    36061 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
    36121 gacagcggtt cgcctctgat gttgcatacc gatgacggct ggcaattaat tggggtgcaa
    36181 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
    36241 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgacag
    36301 cgcgggcagc gtcttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
    36361 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
    36421 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
    36481 acggcctgag aaagcgcact aaaggcagcc agcaccgccg caagggagag caagccaaat
    36541 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
    36601 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
    36661 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
    36721 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
    36781 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatttt
    36841 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
    36901 gccgccattt ccctcactga cgctcgccca tttcatggac agcgtaccgg taatttctgt
    36961 agcaatagcc agacctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
    37021 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgctta
    37081 gcgcaagctt tggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
    37141 gcaagatagt ggatgaggta cgaagatggg taaatgtaga aaataacgtc ctgaacaaat
    37201 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
    37261 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
    37321 atttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
    37381 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
    37441 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
    37501 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
    37561 gtgcaacaca tggtccgctg gcgtgtgccg cgtgtactgg cagtgtcgat tttgatgacc
    37621 atcatcgtga tggcgatggt gttgctatta gcttacttgg gttccgcgct caacgagttg
    37681 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc gcttgaaccg
    37741 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta tattgatccg
    37801 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
    37861 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
    37921 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca gcgtgcgatt
    37981 gacagtgtgt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
    38041 gcatgggcga tgctcgccgc cctcgatgtt cgcttcgctt ttgtctgggg attgctggcc
    38101 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
    38161 caggtactgg tgtttaatgg cttctacgaa gcgttgttgg tgctggcggg atatctgctg
    38221 attaatctgg tcttcggcaa tattctggag ccacgcatca tggggcgtgg gctgggactt
    38281 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
    38341 atgctgcttt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
    38401 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
    38461 ccgtcagggt tacagagctt tcaggattgc atccacgctg gctttggcgt caccaaacag
    38521 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
    38581 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
    38641 aatcggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
    38701 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
    38761 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
    38821 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
    38881 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
    38941 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc
    39001 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
    39061 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
    39121 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
    39181 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
    39241 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt atcagcaatg ccagcacttg
    39301 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
    39361 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
    39421 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
    39481 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
    39541 atagctgtta aagccaacca gcactgccgc cagacccacg aagctatgca ggatcgccac
    39601 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
    39661 aatgaccatc gccagcaaga tccagccaac gttacccgta tccggtccaa aaatggtggc
    39721 gattagcgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
    39781 tttcgaaagc ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
    39841 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
    39901 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
    39961 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
    40021 aaatcgcgtt ggtgaccgac atcagcggtg tatgcagcgc gtgcgataca ttccacacca
    40081 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
    40141 gcgcaacgct tgccagccag ccgaaaagga tgattgccag cgccatcaac gcgtatttac
    40201 gccacggtga acaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
    40261 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
    40321 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
    40381 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgt gaggattgcg
    40441 tcggcagacg gcccggaaga tcggtatagc caatcacttt gacaccattt tccgtagtga
    40501 agatttcacc cggcacggtg tattcacagt tgccgccgtt ttgggctgcc aggtcgacaa
    40561 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
    40621 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
    40681 gttccatttc cgctttgatg aacgcgtccg acatcacttt ggcatagcca tcgccgctac
    40741 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
    40801 tcacttccgg gcgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
    40861 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
    40921 cggccgcagt gatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
    40981 cgcgataacc ggcgatgttc gccatcgagc ttaatgcgtc cagcgattgt gcgcgtgaga
    41041 tacgcggtac agagtccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
    41101 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
    41161 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
    41221 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
    41281 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
    41341 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
    41401 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
    41461 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
    41521 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
    41581 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
    41641 ctgataattg tcttcgacgg tcgggtaaaa cgagacatcg ccccggcacg aatcactact
    41701 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
    41761 ttttttaagc atagacggtc atttgagcag gattaaaaat ggcttaagga atgtgatatg
    41821 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
    41881 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
    41941 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
    42001 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
    42061 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
    42121 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
    42181 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
    42241 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
    42301 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
    42361 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc
    42421 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
    42481 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
    42541 cgaagaaagt tgagatcccg ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
    42601 gcttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcacgctc ccggatggca
    42661 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
    42721 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
    42781 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
    42841 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
    42901 gccgcctttc gcatttttag tcacaaattt ccctgcgacc attgcatgca gacacatctc
    42961 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
    43021 attcttctta tcacgtttta atcactggat atcgatggaa aagaaactgg gactgagcgc
    43081 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
    43141 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
    43201 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
    43261 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
    43321 gggatactgg ctgtgcgcgg tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
    43381 gttaagcttt tttacggaca cgccggaatt gcgcctgttt ggcgatggta atacctggca
    43441 atcgatagtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
    43501 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
    43561 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
    43621 acttgcactt ggcgtacctg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
    43681 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
    43741 tgttggcaaa gcgacactac tggcggttct ctccgctctg ggcgtttact tgctggtaac
    43801 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccatcaat
    43861 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
    43921 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
    43981 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
    44041 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
    44101 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
    44161 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
    44221 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
    44281 gctgttatcc gttgttctgt atgcaccggg gctgctggtt ttcctgtatg cgcgcaaaac
    44341 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
    44401 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagt
    44461 aaaggagata acgatgggta aaacccagcc cttgccaata ttaattactg gcggaggtcg
    44521 tcgcatcggc ctcgcccttg catggcattt cattaatcaa aagcaaccgg tgattgtcag
    44581 ctatcggaca cactatccag ccattgatgg actgattaat gcaggtgcgc agtgtattca
    44641 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagcac
    44701 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
    44761 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    44821 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    44881 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    44941 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    45001 gaattctatt gcgccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    45061 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    45121 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    45181 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    45241 ccaacacgca acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    45301 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    45361 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgatga gcgcaaaggt
    45421 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    45481 caacagcact accagcgcac cgagcgcggc tttgattacc agccccatct ttttacctta
    45541 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    45601 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    45661 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctag
    45721 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    45781 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    45841 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    45901 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    45961 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    46021 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    46081 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    46141 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    46201 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggcctg gatcgtagcg
    46261 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    46321 gcattaaaac tgtgcgtaac aaaggctatc tctttgcgcc tcatgcatgg gaataagcga
    46381 tgaaaaaact gtttatccag ttttacctat tattgtttgt ctgcttcctt gtaatgtctc
    46441 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    46501 atttgatgaa cagttcgctg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    46561 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    46621 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    46681 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    46741 cagttggtcc tgttccttat ctttattacc tccatcagat gcgattgctg gatatcgccc
    46801 tgatcgcttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    46861 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    46921 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    46981 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    47041 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    47101 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    47161 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    47221 acctgccgtt gtggctatca acgcatctgg cagatattca ggcagtaacg cccgataaaa
    47281 cggtacggat aaaaacgctc atgcaaggcc attatgcggc gttggatatg cgcttaatgg
    47341 agcgcgtgct ggataatttg ctcaataacg ccctgcgcta ctgccattca acggttgaaa
    47401 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    47461 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    47521 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    47581 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgcccgcttc tcgtttagct
    47641 ggccgttatg gcataacatc ccgcaattta cctctgcttg acactacgcg cacggtggtc
    47701 aagtcaccac cactgtgcta taaattaagg atgttgtaac taaagtggtt aatattatgg
    47761 cgcgttacga tctcgtagac cgactcaaca ctacttttcg ccagatggaa caagagctgg
    47821 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    47881 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggtg aaacaacatc
    47941 tcggcaacga cgcgcagtcg ctggcgttgc gtcatttccg tcatttattt attcaacaac
    48001 agtccgaaaa tcgcagcagc aaagccgctg tccgtctgcc tggcgtgttg tgttaccagg
    48061 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    48121 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    48181 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    48241 tgcacaaccc cgccacttta cgttttggct gggctaataa acatatcatt aaaaatttgc
    48301 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    48361 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    48421 tgccacagaa cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    48481 tctggtacaa aggagatcaa aaacaagtgc aacacgcctg ccctacaccg ctgattgcac
    48541 tgattaatcg ggataatggt gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    48601 ccgacaatgt gcagcatcgt tataaacctc aggcgcagcc gcttcgtttg atcattccac
    48661 ggctgcacct gtatgttgca gattaacgcc cggctttcat actgccgacc atctgttctg
    48721 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    48781 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    48841 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    48901 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    48961 gcaagcgcac cgattgcagg aaattatgga tcaccatcgg acggaagacg ttcagttcaa
    49021 agttaccgga agcgccaccc atgttgatcg ccacgtcgtt ccccatcacc tgacagcaga
    49081 gcatggttaa tgcctcacac tgtgttgggt tcactttccc tggcatgatt gagctgcccg
    49141 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccggag gccagccagc
    49201 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    49261 gaaccagggc atcacaggtc gccagcgctt caaatttgtt cggcgcggta acaaacggtg
    49321 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    49381 gtccagtacc caccgctgta ccgccaagag ccagttccgc tacgtgaggc aggctgtatt
    49441 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagcg
    49501 ttaacggcgt ggcatcctgc aagtgagtac gaccaatttt gacgatatcg gcaaatgcac
    49561 gggatttctc attcagtgtc tgtgtcaggg ttttaagctg cggaatgagt tgcttgcgca
    49621 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    49681 tgttcacgtc atcgttaggg tgaactttac gttccatccc gcgcacaccg ccgagtaatt
    49741 cactggcccg gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg
    49801 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    49861 cctgacgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    49921 ccgctgcacg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    49981 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    50041 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac
    50101 ctgattaatt ttttctttct gttttgcttt cgttaagcaa cttttagctc acttattatt
    50161 taccatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    50221 ggctctgttt atttcacaca gcgggtgcat tgtgtgagtt gtatctgctg aaagaagtca
    50281 tttcctttat catccacaag aataaacgcc gggaaatctt ccacttcaat tttccagatg
    50341 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    50401 aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca
    50461 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct
    50521 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    50581 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac
    50641 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgggc aatatcacga
    50701 ccaacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    50761 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    50821 ttgcgcagct cttccgggat atatttgcct ggattatgtt ccagtttttc gatccagatc
    50881 ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggagac gcccataccg
    50941 accgggcagg atgcgccgtg acgtggcagg cgaatcacgc ggatgtcgtg agcgaagtat
    51001 ttaccaccaa actgcgcacc cagaccaaga ttttgcgctt cgatcagcaa ttctttttcc
    51061 agttccacat cgcggaacgc ctgaccgtgc tcattccctt ccgttggcag ttcatcatag
    51121 tatttcgcgg aagccagttt caccgtttta aggttcgttt ctgcagaagt tccaccaata
    51181 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    51241 aggtaatttt tcagtttccc cggcgtcagt aacgctttgg tttcctgata gagatacgtc
    51301 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg
    51361 gcataaagat cgatctgcgc tggtagattg gtgccggtat tcacttcttt atacatatcc
    51421 agcggcgcgt tttgcgagta gcgcagatta tcttcgatat aagtgttata gacaccgcgc
    51481 gccagcgccg cttcatcacc accaccggtc catacacgct gccctttttt accaacaata
    51541 atcgcggtgc cggtatcctg acaggttggc agaacgcctt tcgccgcgat gtcggagtta
    51601 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cctccgggtc acgcagaatg
    51661 tcggccacct gttgttggtg cgccggacgc agcataaacg acgcatcatg aaacgcctga
    51721 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    51781 gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    51841 aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt
    51901 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac
    51961 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    52021 ggattcttgc tttaatagcg ggattaattt ccacattaaa acagggattg atcatgcaaa
    52081 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    52141 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    52201 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    52261 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    52321 tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac
    52381 acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    52441 agcgtaacta taaagatcct aaccacaagc ctgagctggt ttttgcgctg acgcctttcc
    52501 ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag
    52561 gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac
    52621 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    52681 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattctttta atttctgaat
    52741 tttacccgga tgacagcggc ctgttctctc cgctgttgct gaatgtggtg aaattgaatc
    52801 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    52861 tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg
    52921 atattccgga actggttgcc aatgtgaagt tcgaagccaa accggctaac cagttgttga
    52981 cccagccggt gaaacaaggt gcagaactgg acttcccgat tcctgttgat gattttgcct
    53041 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    53101 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    53161 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    53221 gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    53281 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    53341 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    53401 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agagatggtc
    53461 gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat
    53521 caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc
    53581 gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    53641 catggtccct tcccccttgc ccagcttaaa aaactgaacc tgatcccatc gatggcatca
    53701 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    53761 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    53821 tcgctcaagc cgctgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    53881 ttccagttaa atgcggacag agacggcaaa gctatctccc tttccgggga ggcgcaaagt
    53941 ggtcggatag acgcggttaa cgaatacaac cagaaagtac agttgacctt taataatctg
    54001 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    54061 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    54121 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    54181 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    54241 aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa
    54301 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    54361 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    54421 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    54481 ctgaaagacc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    54541 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagttgatg
    54601 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    54661 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    54721 accatcacca ccagcctgca atatgctaac ggtcagataa cgttaaatgg gcagaaaatg
    54781 ccactggaag atttcgttgg tatgtttgca atgccggcat taaacgttcc ggtcgtaccc
    54841 gctattccgc agcagtaatt cacagccaaa acgccggagg tttttctctc cggcgttatt
    54901 tcttacttca gcataaagtc atactgaata ctcattttca catcccagta attcaggcga
    54961 tcgcccagcg tactgccatc gcgcttttcc cgccctttat cggcagcaag aaagaaattc
    55021 atcccctgta acggcccttc gctgacccga tagttcatac cacctaagat acccgcgcct
    55081 tcaatccgca catctcgttt actggattca tagccgcgag tcacaatcgg tgcaaatgtc
    55141 agcgtaaatt gcggggttaa acgatagttg acgcccaatt gccaggactg cattccttca
    55201 cggttacgat caagacttaa tgaaaaggga tagccaatgt cggtatccac cacggcacca
    55261 ttcattgcat ggcgcaaata accaccactg ccaaatacgc ttccccacgg cgcattccag
    55321 gtcaattgtc cactaaccaa cgccgtttca ttgggctggc tggtattacg acttaaacct
    55381 tcgagctgcg cgtaaaagcc gagcaactcc cctttcaacg tataattcgg gtcgagcggt
    55441 atgcttttct cgagaatact ttgtatctga ttggaaacat ctttctgtac cgatgccccc
    55501 agagaatagc gactggtatc atcatgccaa ctggcggcta aaaaagaacg tgcgcgatgt
    55561 ttatcgtatt ttcccgtctg gctgttatac accccttcat cacgaatacc ggttgttgaa
    55621 tcagtccata aaaatactcg tgaacgccac attccccaca gatcaatatt tggcgttaaa
    55681 gcccaataac ctaatgctcc ttcagaaact gtcggtaatg tacgttgcga actgccagcc
    55741 attaacggaa tacgataagg taagctacca taactataat ccgtcccgaa gcgacctaat
    55801 ttaagcaggc tattttcact gggttttatt tttactgccc ccagggcaaa gccaaaactt
    55861 ttatcaccat caagatacca ccgggtactc atatcagcac gagcacctaa tttataaaca
    55921 taatacgctc cgccttcaac gccaataata ttggaataat aaccactatt gaaatcgagc
    55981 aatcctccct gtacccatgc gtaaatatct cttcccgcgc cagcactggg cttatcagcc
    56041 ctgcgcaatt cgtttttcag tctgatgcgt agtcccgctt catcgtcagc cagttgcgcc
    56101 gcgtaagcag aggtaaggcc agaggcagcc gtcaggcaaa taacggccat ggccactatt
    56161 tttctcatag ttgcaccttt gatg
//
LOCUS       DAZGCQ010000019        55115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000019 DAZGCQ010000000
VERSION     DAZGCQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.019"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            251..784
                     /locus_tag="ACHK07_001247"
     CDS             251..784
                     /locus_tag="ACHK07_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101606.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            834..1517
                     /locus_tag="ACHK07_001248"
     CDS             834..1517
                     /locus_tag="ACHK07_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8101607.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1530..3980
                     /locus_tag="ACHK07_001249"
     CDS             1530..3980
                     /locus_tag="ACHK07_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8101608.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSLLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3993..4952
                     /locus_tag="ACHK07_001250"
     CDS             3993..4952
                     /locus_tag="ACHK07_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO8101609.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(4990..5889)
                     /gene="nhaR"
                     /locus_tag="ACHK07_001251"
     CDS             complement(4990..5889)
                     /gene="nhaR"
                     /locus_tag="ACHK07_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO8101610.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5955..7121)
                     /gene="nhaA"
                     /locus_tag="ACHK07_001252"
     CDS             complement(5955..7121)
                     /gene="nhaA"
                     /locus_tag="ACHK07_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO8101611.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            7707..7859
                     /gene="mokC"
                     /locus_tag="ACHK07_001253"
     CDS             7707..7859
                     /gene="mokC"
                     /locus_tag="ACHK07_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO8101612.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(7963..9093)
                     /gene="dnaJ"
                     /locus_tag="ACHK07_001254"
     CDS             complement(7963..9093)
                     /gene="dnaJ"
                     /locus_tag="ACHK07_001254"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO8101613.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9182..11098)
                     /gene="dnaK"
                     /locus_tag="ACHK07_001255"
     CDS             complement(9182..11098)
                     /gene="dnaK"
                     /locus_tag="ACHK07_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO8101614.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            11475..11879
                     /gene="yaaI"
                     /locus_tag="ACHK07_001256"
     CDS             11475..11879
                     /gene="yaaI"
                     /locus_tag="ACHK07_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO8101615.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11905..12618
                     /gene="msyB"
                     /locus_tag="ACHK07_001257"
     CDS             11905..12618
                     /gene="msyB"
                     /locus_tag="ACHK07_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8101616.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            12767..13333
                     /gene="satP"
                     /locus_tag="ACHK07_001258"
     CDS             12767..13333
                     /gene="satP"
                     /locus_tag="ACHK07_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO8101617.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(13368..13955)
                     /gene="mog"
                     /locus_tag="ACHK07_001259"
     CDS             complement(13368..13955)
                     /gene="mog"
                     /locus_tag="ACHK07_001259"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO8101618.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(14070..15023)
                     /gene="tal"
                     /locus_tag="ACHK07_001260"
     CDS             complement(14070..15023)
                     /gene="tal"
                     /locus_tag="ACHK07_001260"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8101619.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            15302..16732
                     /gene="yaaJ"
                     /locus_tag="ACHK07_001261"
     CDS             15302..16732
                     /gene="yaaJ"
                     /locus_tag="ACHK07_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO8101620.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            16802..17578
                     /gene="yaaA"
                     /locus_tag="ACHK07_001262"
     CDS             16802..17578
                     /gene="yaaA"
                     /locus_tag="ACHK07_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO8101621.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(17731..18027)
                     /gene="yaaX"
                     /locus_tag="ACHK07_001263"
     CDS             complement(17731..18027)
                     /gene="yaaX"
                     /locus_tag="ACHK07_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO8101622.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(18241..19527)
                     /gene="thrC"
                     /locus_tag="ACHK07_001264"
     CDS             complement(18241..19527)
                     /gene="thrC"
                     /locus_tag="ACHK07_001264"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO8101623.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(19528..20460)
                     /gene="thrB"
                     /locus_tag="ACHK07_001265"
     CDS             complement(19528..20460)
                     /gene="thrB"
                     /locus_tag="ACHK07_001265"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO8101624.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVETAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDARLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(20462..22924)
                     /gene="thrA"
                     /locus_tag="ACHK07_001266"
     CDS             complement(20462..22924)
                     /gene="thrA"
                     /locus_tag="ACHK07_001266"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO8101625.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGLHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(22950..23069)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(23005..23070)
                     /gene="thrL"
                     /locus_tag="ACHK07_001267"
     CDS             complement(23005..23070)
                     /gene="thrL"
                     /locus_tag="ACHK07_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO8101626.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(23284..23970)
                     /gene="yjtD"
                     /locus_tag="ACHK07_001268"
     CDS             complement(23284..23970)
                     /gene="yjtD"
                     /locus_tag="ACHK07_001268"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8101627.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(24370..24510)
                     /gene="yjjY"
                     /locus_tag="ACHK07_001269"
     CDS             complement(24370..24510)
                     /gene="yjjY"
                     /locus_tag="ACHK07_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO8101628.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            24606..25322
                     /gene="arcA"
                     /locus_tag="ACHK07_001270"
     CDS             24606..25322
                     /gene="arcA"
                     /locus_tag="ACHK07_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO8101629.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(25382..26734)
                     /gene="creD"
                     /locus_tag="ACHK07_001271"
     CDS             complement(25382..26734)
                     /gene="creD"
                     /locus_tag="ACHK07_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO8101630.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLGELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDELRIWK
                     "
     gene            complement(26792..28216)
                     /gene="creC"
                     /locus_tag="ACHK07_001272"
     CDS             complement(26792..28216)
                     /gene="creC"
                     /locus_tag="ACHK07_001272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO8101631.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(28216..28905)
                     /gene="creB"
                     /locus_tag="ACHK07_001273"
     CDS             complement(28216..28905)
                     /gene="creB"
                     /locus_tag="ACHK07_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO8101632.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(28918..29391)
                     /gene="creA"
                     /locus_tag="ACHK07_001274"
     CDS             complement(28918..29391)
                     /gene="creA"
                     /locus_tag="ACHK07_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO8101633.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            29602..30471
                     /gene="robA"
                     /locus_tag="ACHK07_001275"
     CDS             29602..30471
                     /gene="robA"
                     /locus_tag="ACHK07_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO8101634.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(30468..31115)
                     /gene="gpmB"
                     /locus_tag="ACHK07_001276"
     CDS             complement(30468..31115)
                     /gene="gpmB"
                     /locus_tag="ACHK07_001276"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO8101635.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            31167..31679
                     /gene="yjjX"
                     /locus_tag="ACHK07_001277"
     CDS             31167..31679
                     /gene="yjjX"
                     /locus_tag="ACHK07_001277"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO8101636.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(31826..32152)
                     /gene="trpR"
                     /locus_tag="ACHK07_001278"
     CDS             complement(31826..32152)
                     /gene="trpR"
                     /locus_tag="ACHK07_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO8101637.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(32242..34179)
                     /gene="sltY"
                     /locus_tag="ACHK07_001279"
     CDS             complement(32242..34179)
                     /gene="sltY"
                     /locus_tag="ACHK07_001279"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO8101638.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            34390..36057
                     /gene="ettA"
                     /locus_tag="ACHK07_001280"
     CDS             34390..36057
                     /gene="ettA"
                     /locus_tag="ACHK07_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO8101639.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(36164..36331)
                     /gene="nadS"
                     /locus_tag="ACHK07_001281"
     CDS             complement(36164..36331)
                     /gene="nadS"
                     /locus_tag="ACHK07_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8101640.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(36365..37597)
                     /gene="nadR"
                     /locus_tag="ACHK07_001282"
     CDS             complement(36365..37597)
                     /gene="nadR"
                     /locus_tag="ACHK07_001282"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO8101641.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(37618..39000)
                     /gene="radA"
                     /locus_tag="ACHK07_001283"
     CDS             complement(37618..39000)
                     /gene="radA"
                     /locus_tag="ACHK07_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO8101642.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(39049..40017)
                     /gene="serB"
                     /locus_tag="ACHK07_001284"
     CDS             complement(39049..40017)
                     /gene="serB"
                     /locus_tag="ACHK07_001284"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO8101643.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            40123..40767
                     /gene="ytjB"
                     /locus_tag="ACHK07_001285"
     CDS             40123..40767
                     /gene="ytjB"
                     /locus_tag="ACHK07_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO8101644.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            40795..41811
                     /gene="lplA"
                     /locus_tag="ACHK07_001286"
     CDS             40795..41811
                     /gene="lplA"
                     /locus_tag="ACHK07_001286"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO8101645.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            41843..42106
                     /locus_tag="ACHK07_001287"
     CDS             41843..42106
                     /locus_tag="ACHK07_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8101646.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(42190..42909)
                     /gene="deoD"
                     /locus_tag="ACHK07_001288"
     CDS             complement(42190..42909)
                     /gene="deoD"
                     /locus_tag="ACHK07_001288"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO8101647.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(42966..44189)
                     /gene="deoB"
                     /locus_tag="ACHK07_001289"
     CDS             complement(42966..44189)
                     /gene="deoB"
                     /locus_tag="ACHK07_001289"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8101648.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(44241..45563)
                     /gene="deoA"
                     /locus_tag="ACHK07_001290"
     CDS             complement(44241..45563)
                     /gene="deoA"
                     /locus_tag="ACHK07_001290"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO8101649.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(45690..46469)
                     /gene="deoC"
                     /locus_tag="ACHK07_001291"
     CDS             complement(45690..46469)
                     /gene="deoC"
                     /locus_tag="ACHK07_001291"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO8101650.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            46727..48277
                     /gene="yjjI"
                     /locus_tag="ACHK07_001292"
     CDS             46727..48277
                     /gene="yjjI"
                     /locus_tag="ACHK07_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO8101651.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            48249..49112
                     /gene="yjjW"
                     /locus_tag="ACHK07_001293"
     CDS             48249..49112
                     /gene="yjjW"
                     /locus_tag="ACHK07_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO8101652.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVEEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(49509..50291)
                     /gene="yjjV"
                     /locus_tag="ACHK07_001294"
     CDS             complement(49509..50291)
                     /gene="yjjV"
                     /locus_tag="ACHK07_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8101653.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(50288..51361)
                     /gene="yjjU"
                     /locus_tag="ACHK07_001295"
     CDS             complement(50288..51361)
                     /gene="yjjU"
                     /locus_tag="ACHK07_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO8101654.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(51483..51644)
                     /gene="ytjA"
                     /locus_tag="ACHK07_001296"
     CDS             complement(51483..51644)
                     /gene="ytjA"
                     /locus_tag="ACHK07_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO8101655.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(51771..52376)
                     /gene="osmY"
                     /locus_tag="ACHK07_001297"
     CDS             complement(51771..52376)
                     /gene="osmY"
                     /locus_tag="ACHK07_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO8101656.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(52769..54355)
                     /gene="prfC"
                     /locus_tag="ACHK07_001298"
     CDS             complement(52769..54355)
                     /gene="prfC"
                     /locus_tag="ACHK07_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO8101657.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            54575..54823
                     /locus_tag="ACHK07_001299"
     CDS             54575..54823
                     /locus_tag="ACHK07_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO8101658.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT        13583 a        14547 c        13488 g        13497 t
ORIGIN      
        1 taattaactg cagagaaata aaaaataatc tgaagcatgc tctgaaacat cctactctgt
       61 aactctaatg atggcacctt gctcttctta taaagaaggg aacaattaag aatattaaat
      121 tttcaaactt tcatcaactt cacatcaaga aattaaaaaa tttaaacatt aatctaaata
      181 ataacaacct gatcacataa tcgggtttga aaacgagtga ccttccatac cagttaatta
      241 aggatgattt atgaaaaagt atattattcc cttcgttgcg acaacgttga tgtttagcgc
      301 aacaaccttc gccaaggatg tcgatcaagg aacactaacc attagaggag aagttgttga
      361 tacaacgtgt tactttgtta atggtgattc gacaacagat atcgttgtcg aagatgttgt
      421 aaccagtgca atgaatcaac ttatcaataa tgaaatctat actttaaaaa ccggttcaac
      481 ctctcatgac ttagaaattc agtgtgacgg aaatacagcc ccaagtatcg aagtactagc
      541 atcagagttt gatgctaata actttacgct gaataaagga actgctgaga acattggctt
      601 tgccattttc ctcgatgatg ggactttgac cagaatgaga ccagggttaa aaacaccgct
      661 gaaagataat ggctctggtc aatattttct taatttgaat gcacaatatg cccgcacaag
      721 tggcaatcca gttacaaaag gctccgtaga gtcaaccgta acgattaaaa tcagtgcaga
      781 ttaacgtttt ttgccggtag ctctgctacc ggtattattt acggagtgat tacgtgagaa
      841 taataacagc attactgatg tcatttttta ttttacctgt tcatgccggt gtggtcatct
      901 atggtacgcg cattatttat cctgaaaaaa acagagaagt tctcgtacag ttaatgaatc
      961 aaagtaaaaa tgcctcttta attcaggcat ggatcgatga tgggaacacc actatcgcgc
     1021 cagaaaaaat tcaggtccca tttattctca caccgccagt aagtcgtgta gcaggtggtt
     1081 ctggccaaca acttaaaata agaaaaatgc caaacaactt gcctcacaat aaagaaagtt
     1141 tattttatct aaatgttctg gatatccctc caaacaaccc acaaaatgca ggaaaaaaca
     1201 aaattaaact tgcattgcaa aaccgtataa agttactttg gcgtccaagc ggaatagctc
     1261 cggtagataa aaaaagcctt tctcaattaa atatcaaaaa gaaaaataac gccatctcaa
     1321 taaacaacga aacagccaac tggataacgg ttacaactat caaagcacaa aacgtgaaag
     1381 ttaataatga aagtatctta ctgcctccat tttcgaataa cgacattaca ttaaaaaata
     1441 accacgcatc agaatatgaa ctaacggtag ttgatgatta cgggaataac atccatagta
     1501 aaatcgccgc gagataacag gaaaaagtca tgttaaggac gacacgatgg gtcgctgcta
     1561 ttattttttt gtattctttt ccgggttatg ctgaagaaac ttttgatacg cactttatga
     1621 tcggcggcat gaaaggagaa aaagtttccg aatatcattt cgataataaa caaccgcttc
     1681 caggaaacta cgaacttgat ttttatgtaa ataaccagtg gcgtggaaaa caagatatca
     1741 cgatcccaga gtcaccagtc aaaccatgtc tgcctaaggt gctactaacc aaactggggg
     1801 taaagaccgg taacctcaat acagaagata attgtatttt actggataaa gcggttcatg
     1861 gtggacaata tcagtgggat atcagtgaac accgactaaa tttaacagta ccacaggcgt
     1921 atatcaatga acttgaaaga ggttacgttc ctccggaaag ttgggatcgc ggcattgatg
     1981 ctttttatac ctcctataat ttaagtcagt atcgctctta tgacagcaat aataacagta
     2041 atacagccag ttatggccgg tttaacagcg gattaaattt atttagttgg cagttacact
     2101 ctgatgccag ttatagcaaa cctgatgata tgaaaggcaa atggcaaagt aatactcttt
     2161 acctggaacg tggctggtcg cagatattaa gcacagttca gataggagaa aattacacat
     2221 cctcactcat attcgactct ctgcgattta gcggaatacg cttatttcgc gatatgcaaa
     2281 tgctacctga ttctatgcaa agctttacac ctttagtaca aggtgttgcg caaagtaacg
     2341 ccttgataac ggtttcgcaa aatggctata ccatttacca aaaagaggtt cccccaggac
     2401 catttactat cgcagatttg caactttcgg gcagcggctc tgatctcgat gtcagcatta
     2461 aggaagcaga tggtagtgtt cgttcctttt tggtgcccta ctcttccgtg cccaatatgc
     2521 tgcaacctgg tgtttcaaat ttcgatttta tcgccggacg tagtcagata tatggtgtca
     2581 agaatcagga ggactttctc gaggcaaatt atatatatgg cctcaacaat cttttgacac
     2641 tttatggcgg tacgatttta tccgataact ataatgctat aactctggga aatggttgga
     2701 atacaccgct gggtgccatc tcttttgacg ctacccgatc gagtagtaag ttaaataatg
     2761 ataccagaca cgaaggtacg agctatcagg tcgcctataa taaatatcta ttgcaaacgg
     2821 ccactcactt tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg
     2881 accatcttta cgaaaatgat aaaataaatc atcagagtga ctatgatgat ttttatgata
     2941 ttggcagaaa aaatagcctt tctgccaata taatgcaacc tttatccaat aatctgggaa
     3001 atgtatcatt aagtgcactt tggcggaatt actgggggcg aagtggaaat gctaaagatt
     3061 accaattcag ttactccaat aactggcaac acattagtta tactttctct gccagccaat
     3121 cctatgatga aaacgataaa gaagaggagc gttttaatct gtttatctcc attcctttct
     3181 actggggtga tgatattgcc aaaacacgtc accaaattaa cttatcgaat tctacctcat
     3241 tttccaaaga tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg
     3301 atcagttaaa ttatggtata tatgttaatc agcaacaaca aaataatgat acctcgcttg
     3361 gtacgaattt aagctggaga actccaatcg ccacaataga tggcagctat agccattcta
     3421 aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta
     3481 ttaatatcac taaccagcta tccgatacat ttgcaattct ggatgcgcct gggttagaag
     3541 gcgcgcatat taatggacaa aaatacaacc gaacaaacag caaaggccag gttgtttacg
     3601 acctgatgat acctcatcgt gaaaaccatc tggtacttga tacagcaaac agtgaaagtg
     3661 aaacagaatt gcagggcaat cgtcaaatta ttgcgcctta ccgtggagca gtttcttatg
     3721 tgcagtttac aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt
     3781 cgccattaac ttttggctat gacgtactgg atctccagga aaacaatatt ggagtcgttg
     3841 gccagggtag tctccttttt attcgcgtag atgaaattcc cactggcata aaagttgctc
     3901 tcaatgatga acagaattta ttctgtacta ttacttttca acacgttatc gatgaaaaca
     3961 aaacatatat atgccagtaa aaggagttta ctatgaagtg gcttttatta atcaccttga
     4021 gtctatattc ttttattgtg caaagtgctc cctgtggatt gaccaatgtc ggggaacaac
     4081 gtggaacata tatactcaag ccactgagta tgaaaggtaa tctcaccgcc gaaaaaattg
     4141 aaaacaccgg aaatatcatt gagtgtgcag acttgctaga gacgactaac gccattaaag
     4201 ttcatttttt aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta
     4261 atttcccctt tgaatctgac ggtaaaaaaa tagagcttcc agggaagtca tcttataatt
     4321 tagaagagat gctatctgcg ataaatttca ccattccata ctctatcgca tctgtatcag
     4381 atagttccaa caccgcagat atttttccag gaactccttt tcctttgttg ttcgaaattg
     4441 aagtgagttc atgtacaggg aataatacta attgtaatag cgtccgcttt aattttaatt
     4501 taaatactat tttacaggta aatttaatga cctgtggttt tgaagatcaa tccatcgaca
     4561 ctggacgatt tgtactgtct cgtatcaata atgaaaatta tcagtttcat catataagat
     4621 ttgactgcat ccagggcgag caaggtgaat tagtttttga acccagtgac gttgaatatt
     4681 actttgaacc agtgacgata caggagtcag gaaccagcat attaaaaaat gagttagaaa
     4741 acagtgagga cggtgcgggt caaataggct ttcaactctc caatgatggt acgaatgaaa
     4801 tccagtatgg taaaagcaat tactactctt ttcagcatcc ccatgaagga agtaatcaga
     4861 ttcctctatt tattcgcccg cgtacatatg gaaacaatgt ctcaagcggg caaataatga
     4921 gccgagtcaa aatcgtggta atgtacaact aatctatctc ccccacctta actttggggg
     4981 agctgccgat taacgcaccg ctggactaaa aagcgcagaa taatccgtat tgcagattcg
     5041 ctgtaccgcc gggtgctgaa tcatccgctc agcaaaaata gcatggtact cttccatcac
     5101 attctcgacg cgaccaattt ctacgacagt tttatcggca taaaagtcat atgcataaag
     5161 cgttggggca acgaagattg cattgtgcat cgcaccaaaa gctttcatca aagcggcatc
     5221 atcaaactcg ccaaggattt ctacgtttaa tccctgggag ttaaaccagt taagtaattt
     5281 gcgccctaac attgaacgtc gcccaggaat caaaagccgc cgttcttcaa gacaagctgg
     5341 gaacggtttt tccggcggtg gatttgtaca ccagaaactc acgccacatt cgccaattct
     5401 cacggagaac aggccttcct gctgcgtaga atctatcgga cagtcggaaa tgatcatatc
     5461 cagtttatgc tggcttaatt gctccagcag catttcgtgg gtggattcga agcagcgaag
     5521 atgaatcggc tcgccttcta ctactgcagc attcagcacg ctactgacca gacgtttgga
     5581 aagtgcatca gccacgccaa cgtcaaacaa taaattggat tctttgcgat agttcacgat
     5641 atccagcatt tcctggctca aggtgaacat tttatcggca tagcgataga ccagctcccc
     5701 cagctcgctg ggctcgagac cacgtccctt gcgcttaaat aatttgcctt gcaggcgctc
     5761 ttccagcgcc cgaatctgtc cggtaatggt ttgtggtgtt aaataaagcg cctccgctgc
     5821 gccaaccaca gaaccttctt tataaacatg ccagaagtaa tacaagtggt tgtaattgat
     5881 atgagacatg ctcatttctc tccctgataa caatgaaaag ggagccgttt atggctcccc
     5941 ggtaaaccgt cctgtcaaac tgatggacgc aaacgaacgc gtaaccagct gtatccaatt
     6001 accgccgaag agatagaacc gaccaggata ccgagtttcg cccagttaat cagttctgga
     6061 tctacgctac caaaggccag gctggcaata aagatagaca tagtaaaacc gataccgcac
     6121 aggatcccca ccgccataat ttgctgataa gtcgttccct caggcagatg cgccagtttc
     6181 aaacgcagcg ccaaccagca gaacagacta atccccagcg gtttgccaat cagcaagcca
     6241 gcgatgatcc ccaatggcag aatggaggtc aagccatcca gcgtgacgcc ttgcagtgaa
     6301 acgccagcat tagcaaatgc aaacagtggt aaaatcagat acgccaccca cgggtgcaac
     6361 acatgctcca gtcgcttcgc tggagaacgc ccatgcttct ctttcaaagg aataaagaag
     6421 ccgacaatta cccccgccag ggttgcgtga acccccgatt tcaacactgc agtccacaac
     6481 actacgccaa ccagaatata gacgcccgtg cggcgtacac cacacagatt caataccgcg
     6541 agtaccgcaa ttgctacagc cgcgacgcca agagaggcca tcgataagtc attagtgtag
     6601 aacaatgcga tgataatgat ggccccaaga tcgtcgataa tagccagagc catcaaaaag
     6661 atcttcagcg ctaacggaac acgacttccc aatagcgcca gcacaccaag tgcaaaggca
     6721 atgtcagtag ccgccgggat cgcccaccct tcgcgggtaa tcggatcggc atagttaaag
     6781 gccagataga gtaatgccgg cacaatcatc ccaccaatag cggcgataac tggaaatgcg
     6841 gcctggcgta agctggctag cgatccttgc atcagttcac gtttaacttc cagaccgact
     6901 aacaggaaaa ataccgccat cagcgcgtca tttatccata acagcatgtt tttgttgatt
     6961 tcgagtgaac caacccggag ctgaaccggc gtctccagaa agtcgtgata ccatccactg
     7021 gttgcgccgc tgttggccat catcatcgcc aggatagcgg caatgataag aataatgcct
     7081 cccgaggcat cactgctaaa gaatcgatgc agatgtttca ctttttattt ctctttcagg
     7141 tgaatagatc gtttttacta ttttaccccg cacgaatcat cgttaaaatc agattatagt
     7201 gtaggaataa ttcgtttttt acgatctata gaagaatttg cgccatcagc ggtgagatgc
     7261 gtttttgacg gctgtaagcg ctgacgggtg accctaagcc ctgataatca tagcttaagg
     7321 tgaggatttc tgagggatta attaggaaaa gtaataatta atttgttgtc agtcggcgat
     7381 gtttgttagg ccaatatttc aaaaataatc cagcttgtcg tcaataattg cgatgaagag
     7441 ataaattaaa gttaaataat taaatcatta aaaaattacg ccataaaaat gcgaggcgtc
     7501 ttccggatta gaatcgataa aacagaatgg gttaaagaga gcgatataat agcggcgggt
     7561 gcttgaggct gtctgtctca ggcattagct gaacggcaga tagagaaaag ccccgagtga
     7621 tattttacca tcaacccgag gccccctata tgctgaacac atgtagagtg cctcttactg
     7681 accgtaaggt caaggagaag agagcaatga agcagcataa ggcgatgatt gtcgccctga
     7741 tcgtcatctg tatcaccgcc gtagtggcgg cgctggtaac gagaaaagac ctctgtgagg
     7801 ttcacatccg aactggccag acggaggttg ctgttttcac ggcttacgaa tccgagtaag
     7861 agcaacggcg gggagtgatc cccgccactt tacaggtgct cgcatatctt caacgcaccc
     7921 tatttttacc caggcctgcc cacgggcagg cttttgggga ggttagcggg tcaggtcgtc
     7981 aaaaaacttc ttcacaccat caaagaagct ctttgagcgc gggctgttgt gctcgccggt
     8041 tgggccaccg aagctttctt gcagctcttg cagcagctgt ttctgcttct cgttcaggcc
     8101 tacaggtgtt tcgacgacaa cgcggcacag caaatcaccc tgtgcgccac cgcggacaga
     8161 cttgacgcct ttaccgcgca tacggaatag cttaccggtc tgggtttcgc caggcacttt
     8221 cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg ccacccagcg ccgccatagc
     8281 gaagttgatc gggacttcgc aatacaggtt gttgccttca cgctcgaaaa tcgggtgctg
     8341 tttaacctga acctgaacgt acagatcgcc tgccggtgcg ccatgctcgc ccgcttcacc
     8401 ttcgcccgca agacggatgc ggtctccagt gtccacccct gccgggattt taacggacag
     8461 cgttttgctg cgctcaacac gaccatgacc atgacatttg ttgcacggat ctttgatcag
     8521 cgtaccgcgg ccctgacagt gtggacaggt ctgctgcacg gcaaagaaac cctggcgcat
     8581 ctgcacctga ccagaaccat gacaggtcgg acaggtctgc ggctgtgtac ctggttttgc
     8641 accgctaccg tggcaaacgt cacactcttc cagagtcgga atgcggatct ctttggtcac
     8701 gccacgtaca gcttcttcga gggtgagctc catgttatag cgtaaatcag caccgcgcgc
     8761 cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg aaaacgtcac caaaaatatc
     8821 gctgaagtct gcgccgccgc caaaaccgcc gccgcccatg ccaccttgct caaacgcagc
     8881 atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg gtcagaactt cataagcttc
     8941 cttgatctct ttaaatttcg cctcggcctc tttgtcaccc tggttacggt ccgggtggta
     9001 tttcatggcc aggcgtttgt aggccttttt gatttcacgc tcttccgctg ttttggaaac
     9061 gcctaaaatc tcgtaataat cttgcttagc catctttttt aaattgcccc tagatgaatg
     9121 cacgggcgga gaggaaattc cccttcgccc gtgtcagtat aattacccgt ttacagggcg
     9181 attatttttt gtctttgact tcttcaaact cagcgtcgac aacatcgtca tctttcgcgt
     9241 tgtttgcaga agcatcagca ccggcagtct gctgctgggc atgttgctgc tgggcgattt
     9301 ccatcagttt ctgggaaacc tgtgccagct cctgcatttt cgcttcgata gcggctttgt
     9361 cttcaccttt cagagcagtt tccagtgcag tcagcgcaga ctcgatagca gttttgtcgt
     9421 cagccggcag tttgtcgcct gcttcttcaa cctgcttacg ggtgctgtgc agcagatggt
     9481 cgccctggtt gcgagtctgt accagctctt caaacttacg gtcagcttcg gcgttagctt
     9541 ctgcgtcgcg taccattttc tggatttcat cttcgttcag accagaagaa gccttgatgg
     9601 tgatcttctg ctctttaccg ctgtttttat ctttcgcgga aacgtgcagg ataccgtcag
     9661 catcgatatc gaaggtaact tcgatctgcg gcataccgcg cggtgccggg ttgataccat
     9721 ccaggttgaa ctgacccaga gatttgttat cagccgcacg tttacgttca ccctgcagca
     9781 cgtggatggt taccgcagac tggttgtctt cagcggtaga gaacacctgg ctgtgcttgg
     9841 tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac accgcccatg gtttcgatac
     9901 ccagagacag cggggtaacg tccagcagca gtacgtcttt tacgtcacca gtcagaacac
     9961 caccctgaac agcagcaccg attgctacag cttcgtccgg gttaacgtct ttacgcggct
    10021 ctttaccaaa gaactcagca actttcttct gaaccattgg catacgagtc tgaccaccaa
    10081 cgaggataac gtcgtcgata tcagatacgg acaggccagc gtcctgcagt gcaactttca
    10141 gcggctcaat ggaacggttt accagatctt caaccaggct ttccagtttc gcacgagtca
    10201 ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt gatgtacggc aggttaacgt
    10261 cggtctgctg agcggaagac agttcgattt tcgctttttc tgccgcttct ttcaggcgct
    10321 gcattgccag cggatcgttg cgcaggtcaa tgccctgatc tttcttgaat tcttcaacca
    10381 gatagttgat cagacggctg tcgaagtctt caccacccag gtgggtatca ccgttggttg
    10441 ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat ttcaataata gaaatatcga
    10501 aagtaccacc acccaggtca taaaccgcga tagtacggtt gccagtacct ttgtccagac
    10561 cgtaagccag cgcagctgca gtcggttcgt tgatgatacg ttttacttcg agaccagcga
    10621 tacggcctgc gtctttggtt gcctgacgct gagcatcgtt aaagtatgcc ggtacggtga
    10681 taacagcttc agttaccggt tcacccaggt aatcttcagc ggttttcttc atttttttca
    10741 gcacttcagc agaaatctgc ggcggtgcca ttttctggcc tttaacttcg acccatgcgt
    10801 cgccgttatc agcagcaata attttgaacg gcatgatgga aacatcacgc tgtacttctt
    10861 cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa cagggtgttt tgcgggttcg
    10921 tcactgcctg acgtttagcc ggctgaccaa ccagagtttc accatcctgg gtataggcaa
    10981 tgatagaagg cgtggtgcga tcgccttcgg cgttctccag cacacgagga gtggtgccat
    11041 ccataatcgc tacacaagag ttggtagtac ccaggtcgat accaattatt ttacccatct
    11101 aaacgtctcc actatatatt cggtcatcat gtggttgtga gtctgtaata ggggcgaaac
    11161 gtctggtttc aactgcccaa tttcattttt ttgcagactc actcactgcg gttgactact
    11221 aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa aaattttttg tgcttttgcg
    11281 cagaaatttc gcgatatggc aggacaagac gtaaaattga cagcaatttt attcatataa
    11341 agaatgaatc gcagtcaagg tattcatcaa aaaattgtaa taaaaagaaa agattacgtg
    11401 cctgaatctt ctctttatca gcagtaaact agtgggtatt catcccccta cctcttccca
    11461 ctaagagaat ccttatgaaa tccgttttta cgatttccgc cagcctggcg attagcctga
    11521 tgctgtgctg cacggcgcag gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc
    11581 gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac
    11641 aactctctgc cgaccatggc gatttacagt taagcggtgc atcggtttat ttcaaagccg
    11701 cccgtagcgc cagtcagagc ctgaatattc cttcagaaat aaaagaaggg caaaccactg
    11761 actggatcaa cattaacagc gataacgaca ataaacgctg cgtctcaaaa atcacctttt
    11821 ccggtcatac ggtgaactca tcggatatgg ccacgctgaa aattatcggc gacgactaac
    11881 gccagaatat agaagccaca aaaaatgaat gttaattacc tgaatgattc agatctggat
    11941 tttctccagc attgtagtga ggaacagtta gcaaatttcg cccggctgct cacccataat
    12001 gaaaaaggca aaactcgcct ctccagcgta ctgatgcgca acgaactgtt taaatcgatg
    12061 gaaggacatc ccgagcaaca tcgccgcaac tggcagctga ttgccggaga attacagcat
    12121 tttggtggcg atagtatcgc caacaaactg cgcggacacg gtaaattgta tcgggccatt
    12181 ttgctcgatg tttcaaagcg attgaagctg aaagccgaca aagagatgtc tacgtttgaa
    12241 attgagcaac agttactgga acaatttctg cgtaatacct ggaagaaaat ggacgaggaa
    12301 cataagcagg agtttctgca cgcggtcgat gccagggtga atgagctgga agagctgctg
    12361 ccgctgctga tgaaagacaa attattggca aaaggtgtgt cgcatttgct ttccagccaa
    12421 ttgacccgca ttttacgcac ccacgcagca atgagcgtgc ttgggcatgg tttgctgcgc
    12481 ggcgcgggtc tgggaggccc tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc
    12541 agcgcctatc gcgtgacgat tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt
    12601 agcgccactc aggtctgata ccggaaaccg gggaaaattt tcattttccc ctcaaaagat
    12661 cgtagacact gccccactgg ctgattatta tgccgcgccc tgaaaacact acagttattc
    12721 agggaaatta tttcaccatt cattcgatga tgatttttga ggaattatgg gcaacactaa
    12781 gttggctaat ccggcaccgc tgggcctgat gggcttcggc atgaccacca ttctgcttaa
    12841 cctgcacaac gtgggttatt tcgctctgga cggtattatt cttgccatgg gcattttcta
    12901 cggcggcatc gcgcaaattt ttgccggtct gctggagtac aaaaaaggca acactttcgg
    12961 tttaaccgca ttcacctctt acggttcttt ctggctgacg ctggttgcga ttctgctgat
    13021 gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc cttggtgtct acctgggtct
    13081 gtggggcgta tttacgctgt ttatgttctt cggcacgctg aaaggcgcac gcgtcctgca
    13141 attcgttttc tttagcctga ccgtgctgtt tgccctgctg gcgatcggta acattgccgg
    13201 taatgccgcg atcatccact ttgccggctg gattggtctg atctgcggtg ccagcgcaat
    13261 ctatctggcg atgggtgaag tgctgaacga gcagtttggt cgcaccgttc tgccgattgg
    13321 tgaatcccac taattgtttt gagatccccc cgctcggggg gattttttta ttcgctaacg
    13381 tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt ctggtgccgt ttcaacgtat
    13441 ggcccttcca gcaactgaat gcagtacggt acgctggcaa aaataccgtg taccacaacg
    13501 ttaccctcag cgtccttcac accttccagc gtctctttaa tagacttcgg ctgaccgggt
    13561 aagttaagga tcagcgcctg tttgcgaatc acacccacct gacgcgaaag gatcgcagtt
    13621 ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc
    13681 gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg
    13741 gtgagcacca gatggcaact catttcatcc accagctcac acaacgtttg ctcgatgatc
    13801 gcctgctcat cggggattaa gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc
    13861 agccattctt ccagcgcagg gatgccttta tcctgataaa cgccactgga tgcgcgatca
    13921 gagatggaaa ctaagccaat acgtaaagta ttcatcaaga tttttccgtt taaacagtct
    13981 cgttaaacag aatgatacac tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt
    14041 gaccgacttc ccggtcacgc taagaatgat tacagcagat cgccgatcat tttttccagt
    14101 ttttcctggt caatagcaaa cttacggata ccttccgcca gtttatctac tgccattgga
    14161 tcctggttgt gctgccacag gaactcggac tcagtgatac gcgccggacg cgctttcact
    14221 tcgccggtgt aagacagttt acgttcgata gccccttcgc tctcagccag ctctttcagc
    14281 agtgccggtg cgatggtcag acggtcgcag cctgccagtt ccagaatttc gccgatgtta
    14341 cggaagcttg cgcccataac cacggtttca taaccgtgct ctttgtagta ctggtagatt
    14401 tcagatacag aaaccacgcc cggatcttct gccggagcgt actctttctt atcggtattc
    14461 gctttgtacc agtcaagaat acggccaaca aatggagaga tcagaaacac gcccgcttcc
    14521 gcacaagcac gagcctgagc gaaggagaac agcagggtca ggttacagtt gataccttct
    14581 ttttccagct gttccgcagc acggataccc tgccaggtag aagccagttt gatcagaata
    14641 cgatcgttgc taatacctgc atcgttgtag agtttgatca ggcgttttgc tttcgcaatt
    14701 gacgcttcgg tgtcatagga aagacgcgca tcaacttcag ttgagatacg gcccggaacc
    14761 agtttcagga tttccagacc aatatttact gccagtttgt cagtcgcgtc cacgatctgc
    14821 tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag catcatcaat caacttacga
    14881 tattccggaa tctgcgctgc gttaagaatg agagaagggt tggttgtggc atcctgcggt
    14941 tgatacagct tcattgccgc gatgtcccca gtgtcggcca ctacggtggt gtactgacga
    15001 agggaggtca atttgtccgt catgatagta tttctcttta aacagcttgt tagggggatg
    15061 taaccggtct gccctgatga tatcacgacg caacgtcagc gcaaccgccg acaagacggt
    15121 tatcgccagg cgagactgtt tcggatttct gacactgcgt tgatatcgcc cgccattttt
    15181 aaacaaaacc tcatgtatgc tacgcagaag ttatcaagta cctcgtagcg tatatacttc
    15241 ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga ccggcaataa gagggatatg
    15301 catgccagat tttttctcct ttattaacag cgtcctttgg ggatcggtaa tgatttacct
    15361 gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat
    15421 ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca cagccaggcg gtttaacctc
    15481 atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt
    15541 tgcgctggct attaccgccg gtggacctgg agccgtcttt tggatgtggg ttgccgcgtt
    15601 tatcggcatg gcgacctcgt ttgccgaatg ttccctggca caactttata aagaacgtga
    15661 cgtcaatggg cagtttcgtg gcggaccggc atggtatatg gcgcgcgggc tggggatgcg
    15721 ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg
    15781 agttcaggca aacgctgttg cccgagccct gagtttttct tttgattttc ccccgctggt
    15841 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
    15901 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtac tgaccagcct
    15961 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
    16021 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
    16081 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
    16141 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggaa ttgtccagat
    16201 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
    16261 ggcgggtaac ggcacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
    16321 gcgggtgcta atgggttctt ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
    16381 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
    16441 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
    16501 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
    16561 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
    16621 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
    16681 tatcggtcgc cagctttctc gggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
    16741 atcgatcaac tcattctcgt tttttgctaa agtcggcata aatttcctgc aaggactgga
    16801 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
    16861 gcgatataca ctgccggagc tgttagacaa ttcccagcag ttgatccatg aagcgcggaa
    16921 actgacgcct ccgcagatta gcacgctgat gcgtatcagc gacaaactgg caggtatcaa
    16981 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggcgat
    17041 tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca gcgaagacga
    17101 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
    17161 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
    17221 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    17281 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    17341 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    17401 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    17461 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    17521 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    17581 ttgtagtcaa tgccggatgc ggcgtggacg ccttatccgg cctacaaaag catgcagatt
    17641 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgcta
    17701 aatgcaggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    17761 gccgtgatga tcatgatgag ctttcttatg gtgacgcggc ggtggcggcg gtccgtgtgg
    17821 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    17881 accgccatcc cagtaataac cgcgattatc acgatcgcct atctgtaatt ttactgacgg
    17941 gactaacgta atttccgcag cctgcgctgc catgggagcg accagaacca gggaaagtgc
    18001 gagtacaata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    18061 cgatatccgg agattactcg atgagaacgg cggttgttat tctccgcaat cctaatctct
    18121 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    18181 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagata
    18241 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgcga
    18301 aagcaagggt aaatcggcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    18361 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    18421 ttcgcctgga ttcaactggt cacgcagcgc acgatacgct acggcagcgt gcggctccga
    18481 agtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    18541 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    18601 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    18661 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    18721 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    18781 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    18841 tgcttcaaag tagtagcaaa tctgcgccag caaccggctg atgttaatcg agttagccga
    18901 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    18961 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca atgtacagaa
    19021 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    19081 cggtaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    19141 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    19201 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    19261 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    19321 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    19381 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa
    19441 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcatt
    19501 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    19561 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    19621 ggcaacgcgc tgggcggtat ccggcttgtc acacagagcg aacaaggtcg ggccggagcc
    19681 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    19741 aggcagtaac cgttcacggt agggttctgc gataacatct ttcatcagct tcgcggcaag
    19801 ctcaggctga cgggaatagc aagcgtgaat gaagcctgcc agatgtcgcc cgtgcgcaat
    19861 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    19921 aatccccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    19981 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    20041 gtaatgaatg ctgccggaga tacgcccttc cagctcgccc atcaaagcca gcaaacgagc
    20101 gtcattaagc ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    20161 ggcgctggag cctaagcccg aaccgatcgg catattcttt tccagagtca tcgccactgg
    20221 aatttgcttg cccagctcct ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    20281 tggttctgac ggcagcttat cggcaaaacg tccgaggttg ttgaggctga atgtctctgc
    20341 cgtctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    20401 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    20461 gtcagactcc tagcttccat gagagggtac gtagcagatc ggcaaagaca ccggcagcgg
    20521 taacgtcatt gcccgcacca tatccgcgca gcaccaatgg cagcggctga taatagtggc
    20581 tatagaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttaccatcca
    20641 cttcggcaat cttcacgcgg cagacgccat cttcatcaat attgccaaca tagcgcaaaa
    20701 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    20761 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcactggtt
    20821 caatttcaat atccgccaac tccagttcac gtcccgtttc acgagcgaga attaatagtt
    20881 tacgcgctac atccatacca gaaagatcat ctcgcggatc cggttcggta taacccattt
    20941 cccgcgccag agtagtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    21001 aagaaagcga acctgaaaga atgccggaga acttcatcaa ttcatcacca gcattgagca
    21061 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    21121 tacgtcgcga tttttccgcc gcatgacgca acagatggta gtaatccatc gacgaggtgt
    21181 tggccttttt gttcggcgtg acaacgtgca aaccttcgcg caggaagtcg gcatattgat
    21241 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    21301 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgcgcc agttcttcct
    21361 gccagttttc caggtttagg ccatgcacat tggtaagcag agccttcgag ttagcaacac
    21421 cgcagacacg taagtcgata tgtttattct tcaaccagct ttgctgacgc ttcagttgct
    21481 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    21541 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    21601 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    21661 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    21721 ccaccgagat aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca
    21781 gttccaggta gaactcttcc tgcattgccc gttcagctcg cacacagtcg ctttgcggaa
    21841 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    21901 cgcgtgacat tgcagcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    21961 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgt
    22021 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    22081 tcaggcacgg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    22141 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgatttcaac aacctcgcat
    22201 cgggcacctg acgcgggtcg caggtataaa ccccgtcaac gtccgtccaa atctcgcaac
    22261 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    22321 gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc tgccatcagc accatgtgat
    22381 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    22441 cgaggtaatg ccccactgcc agcagttttt cgaccggatc gataacggta acgttgtgac
    22501 cacgcgcttc taacacgccg gccataatgg cgattgacat tttctcgcca cggcaaatca
    22561 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    22621 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    22681 gggcggcggc gagtcccgtc agaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    22741 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggttattt
    22801 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    22861 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    22921 gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc
    22981 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
    23041 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
    23101 tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
    23161 tttactcacg gcaggtaacc agttcagaag ctgctgtcag agactctttt tttaatccac
    23221 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
    23281 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
    23341 tcgttgctct aaaagcccca ggcgttgttg taaccagtcg accagtttta tgtcatctgc
    23401 cactgccaga gtcgtcagca atgccatggc tcgttcgcgt aaagcttgca gttgatgttg
    23461 gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt gatagcaata
    23521 gaccatcacc gcctgcccaa gattgagcga aggataatcc gccaccatcg gcacaccagt
    23581 aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
    23641 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcggcacca gttctactgg
    23701 cgtggcgtag taatgatatt tcgcccgact gcgtgcagtg gtggcgacag tgaaatcgac
    23761 atcgtgtaac gattcagcca gcgtcgggaa aactttaata ttatcaataa tatcaccaga
    23821 tccatgtgcg acccagcgag tggctggctc cagatgtgcc tgactatcga caatccgcag
    23881 ttcgctaaac cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
    23941 gggtgcgacc agaataatcg ttatacgcat attgccactc ttcttgacca aatgaccgcg
    24001 aacagggtga tcactgccaa cttattacgc ggtgcgaatt tacaaatcct taacgtaagt
    24061 cgcagaaaaa gccctttact tagcttaaaa aaggctaaac tatttcctga ctgtactaac
    24121 ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
    24181 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
    24241 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
    24301 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
    24361 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
    24421 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
    24481 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
    24541 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
    24601 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
    24661 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
    24721 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
    24781 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
    24841 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
    24901 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
    24961 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
    25021 tcaatggttg ggaactggac atcaacagcc gttcattgat cggccctgat ggcgagcagt
    25081 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
    25141 ttcagtcccg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc
    25201 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
    25261 cggaaatcat cgccaccatt cacggtgaag gttatcgctt ctgcggtgat ctggaagatt
    25321 aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt atttttcaac
    25381 cttatttcca gatacgtaat tcatcgtccg ttgtaacgtc tttactggct ttcattttcg
    25441 gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca
    25501 ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc agtccccaca
    25561 tcacaccatc cagcaacaac agcgcgaggg taaacaacat gctgttgcgc cagcctttca
    25621 ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg attatccatg
    25681 ccacggtaaa accggtatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg
    25741 aaagccccac cagcaaatat tgcattgggt gtaagcgttg cgcggtgagc gtttcaaaaa
    25801 caaagaacgc cataaaagtc agcgcaatca gcagaatggc gtacttagtc gcccggtccg
    25861 ttaattggta ttgatcggca ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc
    25921 agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt
    25981 gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggcaaa aaatcaccta
    26041 aaaaactggg atgcggccag ttgctggtta aggtcatttc gctattacgc ccggcaggca
    26101 ccacagaaag atcgccggta ccgcttaaat tcagggccat attcagcttc aggttctgct
    26161 tgcgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg
    26221 tgccgggttc aatggtcagc gccgttccgt taacttcagg cgctttcacc acaccaatac
    26281 cacgcgcatc cccgacgcta atcacaataa atggcttgcc aaaggtgata tttggcgcgt
    26341 cgagctcgcc aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga
    26401 cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca
    26461 atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt
    26521 cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg agtttttgcg
    26581 gcccactggt actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga
    26641 caatcacctg ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaggg
    26701 tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca atggcagcag
    26761 gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg
    26821 aggccagcac gccgccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg
    26881 cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca
    26941 aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat
    27001 ccagcacctt aagcgtgacg tgttcatgat ccacttcggc gcttagcgtg atgcaaccgc
    27061 tctcgggagt aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc tccagtaacg
    27121 ccggttcagc agcaacgtta acctcagtag gcataacatg caaagtgatg tttttttctg
    27181 ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca tcaacagcag
    27241 tcagaactac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tctaccaatg
    27301 cctgcatacg cgcattttgg gtcagaatgt tgtcggtaaa acgagccacc acttccggtg
    27361 gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct
    27421 catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca
    27481 tactttccag cgcctgcgca agcttacgca gctcgctact gccgagttca ggaagaggaa
    27541 cgggcttatt gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg gagcggttga
    27601 tccaccaaac catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg gcgctggccc
    27661 ataaaattcg ccgctcgctg cgcttaatga ccggagccat cgccgcgttc ggtttgccta
    27721 cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcactg
    27781 aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga ccacgcaacg
    27841 ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga
    27901 acaatacttt gccctgcgca tcggtcatat agacatgata ttcattgcgc actttgttaa
    27961 tgccaccgat attggcgcga aacgggcgat gttgtagctg attaaacgcc tgcgccagtt
    28021 gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg
    28081 cggtgtcgat cagcgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa
    28141 aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcaacaacc
    28201 gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat
    28261 cggtgaaaga tcggggttga tggcgcgcag cttggcacgc aacgttttaa tgtgggtatc
    28321 gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatta gttgctggcg
    28381 ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt
    28441 cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg
    28501 accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg
    28561 caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat
    28621 ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa
    28681 cgccggatgg agcgccagta actggcggca caattcaaag ccgctgatat caggcaaacc
    28741 aacatcgaga atcatgacat cgggaacctg ctggcgagct ttatccagca ccggcaagcc
    28801 tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc
    28861 tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgcataaatc gcctctgtta
    28921 ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac
    28981 tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcatcataaa agcgcacgac
    29041 ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt
    29101 gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag aaatggccgc
    29161 atcggaggta tcttccgcca ggcccaatcc ccctttaata cccccggttt tcgcccggct
    29221 cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg
    29281 atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg catgagccaa
    29341 tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc
    29401 atttttatct attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac
    29461 aaaatctcaa tacttttatt tccgtcaaga cctaaaacat actctactaa gaaaaaaaca
    29521 ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg
    29581 ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg
    29641 ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat
    29701 tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat
    29761 attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt
    29821 ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag
    29881 aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt
    29941 attcgcccgc cgctgcgtct gggtgaattc accatgccag agcacaaatt tgtcaccctg
    30001 gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct
    30061 gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc
    30121 attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa
    30181 caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg
    30241 gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga
    30301 accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat gctcaacctg
    30361 acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc caaagcggga
    30421 gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta acgctgcagc
    30481 tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac gacccagcca
    30541 gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt
    30601 aagcggcgct ctgcccatgc tggtaatccg agaatcgtac tcaccagaca tcccagtgca
    30661 ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt
    30721 gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccatca
    30781 acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata
    30841 tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtca
    30901 cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg
    30961 atgatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca
    31021 cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc
    31081 gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa
    31141 cgttaatttt ctgagtaata ctgattatgc accaagttgt ctgtgcgacc accaatcccg
    31201 ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata
    31261 ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt gaggaaacgc
    31321 gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt
    31381 gggtggcgat tgaagctggc attgatggcg acagcacttt cagttgggtg gtgattgaaa
    31441 acgccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttgcctgcg gtgattctgg
    31501 aaaaagtgcg tgaaggtgaa gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg
    31561 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
    31621 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcataatgcc gtgtattaag
    31681 cgcctgatgc gacgctgccg cgtcttatca tgcctaccaa acatattgaa attacgggta
    31741 tttgtaggac ggataaggcg ttcacgccgc atccggcatt cggtgcacga tgcctgatgc
    31801 gccacgtctt atcaggccta caaaatcaat cgcttttcag caacacctct tccagccact
    31861 ggcgcagctc gacgggcgcg gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc
    31921 ctgcgccgag ttcatttttt aactcacgct ggctcatttc gccgcgcaac agctcttcga
    31981 caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca
    32041 acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact
    32101 cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc ataatatgtc
    32161 gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa agagtacgat
    32221 agcatatcat aaacgtgtgg atcagtaacg acgtccccat tccgtggcgc tcatcaacgt
    32281 cggtttatcc cccatgaaat agcggtagta agcgtcataa gccagcacgt tcttcacata
    32341 accgcgcgtc tcggagaatg gaatactctc gacaaatgcc actgcgtcga tacgcccggc
    32401 gctgttgcca agccaggttc gcacccgccc tggtccggcg ttataagctg ctgaggagaa
    32461 aatacgatta ttgccaaatt gctgataaac atattgcagg taactggtgc caatgttgat
    32521 attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag agaacatctt
    32581 caccgtatgg gtcgccgtgc ccggcataat ctgcatcagg ccgctggccc ctaccggtga
    32641 tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat agctttgcgg
    32701 gatctcctta ccgctggtgt agcgtttgaa aagatcgttg taagccagcg ggaatcgctc
    32761 ttccagatga tcccacaact tcccggcgat cgttgcctga acgctaagat cccaccattg
    32821 gttgttgaaa gcataccgcg ccagttgagc ttgctctgtt tttgacttgc tcttcaccag
    32881 attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact cgcgcacgcg
    32941 cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt tatcaatctt
    33001 cagctcatac tcttctccga tgcgttgcgc tgcaaccatc gggtagaaac cacgctgttg
    33061 catgagttga tgcaaaatct cttttgcttc agcttcacgt ccgcgttcca gcaataaatc
    33121 cgcctgccag taacgccatt catctttctc tttcgcctcc atcggcaaac gcgccagcca
    33181 ggtattcagg ccgcgacgat cgccagtgcc aagcgccatt cgcacacggc gttcaatcaa
    33241 cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct cgtcggtgac
    33301 atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct gatcttcatt
    33361 aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcag catcctgccg
    33421 cgccacactg gcaaacgcca ccgccgccat ttgacgggta aaatcggtcg ctccggttgt
    33481 acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg ccgaggcgat
    33541 agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag tgttacccgc
    33601 tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac ctgacgcacg
    33661 ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta gccacagctc
    33721 tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat agtagtaatt
    33781 acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc cacgccagtc
    33841 ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt
    33901 ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca ggtcatcggt
    33961 gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag gcatcatttg
    34021 ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt aacgactacg
    34081 ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac agacaccggc
    34141 agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct ctaagtgtaa
    34201 atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca ttgccgttta
    34261 cgccacgtta cggacacttt tttactttta ctgcgaaggt gatggaccaa ctgggattag
    34321 gcggggaaaa aggctacact tcgcctttga aaactcatta tcacgataaa catagaggcg
    34381 aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg
    34441 aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc
    34501 ctgggtctga acggcgcggg taagtccacc ctgctgcgca ttatggcagg cattgataaa
    34561 gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttatct gccgcaggaa
    34621 ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt ttcagaagtg
    34681 gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat
    34741 tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt
    34801 cataatctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc ggactgggac
    34861 gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg
    34921 ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga tgccgaatcc
    34981 gtggcgtggc tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc gattacccac
    35041 gaccgttact tcctcgataa cgttgcgggc tggatcctcg aacttgaccg cggtgaaggt
    35101 attccgtggg aaggtaacta ctcctcctgg ctggagcaga aagatcagcg tctggcgcag
    35161 gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt
    35221 cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg
    35281 aacagcaccg aataccagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg
    35341 cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg cgatcgcctg
    35401 ctgattgatg acctgagctt ctcgatcccg aaaggggcga tcgtcgggat catcggtccg
    35461 aacggcgcgg gtaaatcgac cttgttccgt atgatctccg gtcaggaaca gccggacagc
    35521 ggcaccatca ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca
    35581 atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat catgaagatt
    35641 ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa aggggttgat
    35701 cagggtaaac gcgttggtga actctccggt ggtgagcgcg gtcgtctgca tctggcgaag
    35761 ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caactaacga cctggatatc
    35821 gaaaccctgc gcgcactgga aaacgccctg ctggagttcc ctggctgcgc gatggttatc
    35881 tcgcacgacc gttggttcct cgaccgtatt gccacgcaca tcctggacta ccaggatgaa
    35941 ggtaaagttg agttcttcga aggtaacttt accgagtacg aagagtacaa gaaacgcacg
    36001 ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc
    36061 gtaaaatgcc ggatgcggcg cgaacgcctt atccggccta caaaaccagc aatttcaata
    36121 cgttgtaaaa ctgtaggcct gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg
    36181 aatgccggat gcggcgtgaa caccttttcc ggtctacaac agccttagca tcctcaattt
    36241 cgtggcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca gcctcttcca
    36301 gagaggtttt caactcatca aaaaaactca ttttatacct cgcctttgag cagtttcatc
    36361 acggttatct ctgctccccc atcatctccc gcaccagctc cacgcagcgc aggaaacggc
    36421 tgtcgtaatc ttcctcttca acccgcacaa attcgatatt gttctcttcc agcatctcca
    36481 ccagcagatt ctggaactct ttacgatcca ccgaactgcc gaggctgcgt aaaccatccg
    36541 ccacccacgg cgtgttgttc tccagcagga tcaccagatc gaaacggtat tcatcaatca
    36601 gcgcctgcac gaacggatgt tcccgccctt cgtacttttt gcagaacgcc tgagtggtga
    36661 caaaatcggt atcgataaat gccactttat tggcatattt caccgcaaaa tcaatgtatt
    36721 gagcgtggcc cagcgcgatt ttatcgtagt cagaatactg caatgcgatc tcatcaccgc
    36781 cgaggtgtga aaagacataa tcgcggccat attcccacgc actggtggtg ttgaagatat
    36841 tggcaagttt gtttaccagg gtggatttac cgctcgactc gccgccaagg atcgccacgg
    36901 tacgcacaaa aaacggcttc acttcggtag gaatatattc ccagtagcgg aacgggtttt
    36961 cgcggatctg cgcaccgctg atactcataa aggtacgttt cggatcgacc agcaccgtct
    37021 cgatccccag atgttccata tactgtggcg catcggcttc ttccgaggtg tagatcagat
    37081 ccggctggat ccctttttca gccataaact ttttgatacc gttgctccac acatcccagc
    37141 cgtgcggata cggctccatg ccctcttcgt tgaaagcatg aatgcgaata tttttctgat
    37201 atttaaaagt ttgcaataac cagcgcagac gatccggcac ggtcggctgc tgcgacatgg
    37261 cactgtcttc gaacaacgcg cggtcacggg tatcgtcaaa acccataatg atatgcagct
    37321 cgtcaacctg gctacaggcg cgctggataa ggtagatatg tccggtatgc agtgggtaga
    37381 acttaccgaa tacgacaccg atcgttttct tctgccgggg aaactcaagc cccaaaaaac
    37441 ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttgat tttggcattc agtaactggc
    37501 ttaaataccc tttggtcata ccgctggcat cagctacctg ctgtagcgtg cagccctgtt
    37561 gcttgatggc agttttcagg taatcaaatg acgacatatc tccctccgta tttctcatta
    37621 taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc
    37681 ttccggcgct tttttcggta cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt
    37741 cgccgcttca gagattcgtt cctgaccgct gggcaccggg cggatctccc ctgccagccc
    37801 gacttcacca aacaccacca gatcctgcgg tagcggtctg tcacgcaggc tggaaaccat
    37861 cgccagcagt aacgctaagt cggcgctggt ttcggttacc tttacgccgc cgaccacgtt
    37921 cacaaacaca tcctgatcgg ccatttgcag accaccgtga cggtgcaaga cagccagcag
    37981 gattgccaga cggttttgtt ccagccccac tgccacgcgg cgcggattcg ccatcatcga
    38041 gtgatcgacc agcgcctgaa tctccaccag caacggacgc gttccttccc acaccaccat
    38101 cacggagctg ccggaggtca cttcatctcc gcgacttaag aaaattgccg aagggttgct
    38161 gacttcacgc agcccctgtt cggtcatcgc gaagacgccc agctcattca ccgcgccgaa
    38221 gcggttttta tggctgcgca aggtgcgaaa acgggagtcg gcatcgccat ccaaaagcac
    38281 cgaacagtcg atacagtgtt ccagcacttt cgggccagcc agcgaaccat ctttggttac
    38341 gtgccccacc atgacaatcg ccacaccgcg cgttttggcg aagcgtgtca aataagccgc
    38401 cgtttcacgc acctgcgcca cgctaccagg cgatgactgt acatccgcca tatgcatcac
    38461 ctggatcgag tcaattacca tcagcttcgg ttgctcttct tcggcaatca ggcagatctg
    38521 ttcgatgctg gtttccgaca acatattgag attgtcagtc ggcaggccaa ggcgatgagc
    38581 gcgcattgcc acctgttgca gcgactcttc gccggtgaca tacagcgttt tcatctgctg
    38641 ggccagtttg cacagcgttt gcagtagcag cgtggatttc cccgcaccag ggttaccgcc
    38701 aatcagaatg gcacttcctg gcaccacgcc gccgcctagt acgcggtcga actctttaaa
    38761 tccggtggaa aaacgcggca gctcttcaag gctgatatcg gagagtttct ggacttttgc
    38821 caccccggcg ctaccggcat agccgctgag acgctcgtta cgcgccaccg taggcgacgc
    38881 agcaagacgc acctcggtga tggtgttcca ggcatgacag gcactgcact gcccctgcca
    38941 gcgcggataa tcggccccgc attcattaca aacaaaggcg cgttttggag cttttgccac
    39001 ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg gcgagcaatt acttctgatt
    39061 caggctgcct gagaggatgc agaatacccc catcaggtca gcgtgacgga tggtgacttc
    39121 cgccttttca ttcacttttg gcttggcatg gtaggcaatc cccagccctg ccgctttgat
    39181 catcggcagg tcattggctc catcgccaat cgccacggtc tgcgccagcg ggatttcata
    39241 ctcctgcgcg aggcgagtca gagttttcgc tttgtactgc gcgtctacga tgtcgccgat
    39301 cacattgccg gtaaatttac cgtccatgat ctccagttca ttggctacca cggctgtcag
    39361 gcgcagcttg tcgcgcaggt attcagcaaa gaaagtaaag ccgccggagg caatcgccac
    39421 tttccagccc agcgtttcca gcttgagcac cagttgcgtt aagcctggca tcagcggcag
    39481 attttcacgc acctgttgca gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct
    39541 gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta cttccgccac
    39601 catctcgccc gttccggcca gtttggcaat ttcatcaata cattcaatct ggatggcggt
    39661 ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt ttcccagcgg
    39721 cgcgacatcc agctgcgctt cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc
    39781 cagacgaatc acctgataat cttccacgca ccaggcggca acaatcacca tcgccgcacc
    39841 cagtttgctc tggtattggg tcagacgctg tttatccagc ccacgaccat acagcagcca
    39901 gccgctacga cctgcgtggt aatccagtgg catcacttca tcaccactta atgaaagagg
    39961 cagacccggc cataaagaga catcttcagg caggtcgcac caggtaatgt taggcattaa
    40021 ggctcctgta aaatcgttcg aagcagggaa aataacgcat gaggctacct tgtatccatt
    40081 gcttctggca acattaagtc tcaaattttc aaagggtgga agatggctcg cacaaaactg
    40141 aaattccggc tacatcgggc agtgattgtc ctgttctgtc ttgccttgtt agtggcactg
    40201 atgcaggggg cgtcatggtt tagtcaaaac caccagcgac agcgtaatcc acagctggaa
    40261 gaactggccc gcaccctggc gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc
    40321 gactcaccgg atgaaaaacg cattcaggcg atcctcgatc agttaacgga tgaaagccgt
    40381 atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta tcgcccgttc tggcgaaagc
    40441 gtcgaagtgc gcgaccggct ggcgctcgac ggcaaaaaag cgggcggcta ttttaaccag
    40501 cagattgtcg aacccattgc cgggaaaaac ggaccgctcg gctatctgcg cctgacgctc
    40561 gacacccata cgctcgccac cgaagcccaa caggtggata acaccactaa cattttacgc
    40621 ctgatgttgc tgctctcact ggcaatcggc gtagtgctga cccgcacgct gctacagggt
    40681 aaacgtaccc gctggcagca atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa
    40741 gaggaagaaa gcgagaaaaa agagtgaccc attactacaa gaaaggaaat cgttatgtcc
    40801 acattacgcc tgctcatctc tgactcttac gacccgtggt ttaacctggc ggtggaagag
    40861 tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt ttctctggcg caatgccgac
    40921 acggtagtaa ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg gcggatggaa
    40981 gaagataacg tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt ccacgatctc
    41041 ggcaatacct gttttacctt tatggctggc aagccggagt acgataaaac catctccacg
    41101 tcgattgtgc tcaatgctct gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac
    41161 gatctggtgg tgaaaaccgc cgaaggcgac cgcaaagtct caggatcggc ctatcgcgaa
    41221 accaaagatc gtggcttcca ccacggcacc ttgctgctca atgccgacct cagccgcctg
    41281 gcaaactatc tcaatccgga taaaaagaaa ctggcggcga aaggcattac ctcagtgcgt
    41341 tcccgcgtga ccaacctcac cgagctgttg ccggggatca cccatgagca ggtttgcgag
    41401 gccataacca aggccttttt cgcccattat ggcgagcgtg tagaagcgga aatcatctcc
    41461 ccagacaaaa cgccagactt gccaaacttc gccgaaacct ttgcccgtca gagtagctgg
    41521 gaatggaact tcggtcaggc tccggcattc tcgcatctgc tggatgaacg ctttacctgg
    41581 ggcggcgtgg aactgcattt cgacgttgaa aaaggccata tcacccgcgc ccaggtgttt
    41641 accgacagcc tcaaccccgc gccgctggaa gccctcgccg gacgactcca aggctgcctg
    41701 taccgcgcag atatgctgca acaggagtgc gaagcgctgt tggttgactt cccggaacag
    41761 gaaaaagagc tacgggagtt atcggcatgg atggctgggg cggtaaggta actcgctgat
    41821 taaaatatcc aaggaaggaa atatgatcga tctggaaaac caggaacggg agattatcaa
    41881 tttaatgttc agccagcgta tttcctggct agccgccgtg cgtatcaggc ataaattatc
    41941 gcttgctgaa gtaagtaaaa tgttaggtat ctcgattaat tctctaaaac agatcgaaaa
    42001 gacggaacgg cttagctcaa acatcaaaag caaaatggca gaaatttatg gttgtccacc
    42061 agagttgctg atatgccctt cctggatgac ggctgaacat aaatgaatag attcgtgaat
    42121 agatactaaa aagccggagc agtcccccac tccggcttaa cacgacaatc gccttgcagc
    42181 gaaacacaat tactctttat cgcccagcag aacggattcc agtgcgattt tgatcatgtc
    42241 gttgaaggtg gtctgacgct cagcggcagt ggtctgctcg tgagtgcgga tgtggtcaga
    42301 cacggtgcag atggtcaggg ctttcgcgcc aaattctgca gcgacgccgt agataccagc
    42361 cgcttccatt tccacgccga ggatgccgta tttttccatc acgtcgaaca tttcgccgtc
    42421 cggagagtag aacaggtcag cggagaacag gttacccacg cgagcatcaa tgcccagtgc
    42481 tttagctgca tctactgcgt tacgcaccat gtcgaagtca gcgatagctg cgaagtcatg
    42541 gtctttaaag cggatacggt taactttgga atcggtgcag gcacccatac caatcaccac
    42601 gtcacgcagt tttacgtgcg gcagaacagc gccacaggaa ccaacgcgga taattttctt
    42661 cacgccgaaa tcggtgatca gttctttggt gtagatggag caggacggga tacccatacc
    42721 gtgacccatt acggaaattt tgcggccttt gtaagtacca gtgaagccca gcataccgcg
    42781 tacgttgttc acttcacggg catcttcaag gaaagtttca gcaatatact tcgcacgcag
    42841 cgggtcgcct ggcatcaaaa ctacgtcagc gaaatcgccc atttctgcat taatgtgtgg
    42901 ggtagccatt gttttatcct ttttgtgaca taacaaaggc ggagtcaacg ctccgcccaa
    42961 atccatcaga acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga
    43021 gtctggccga tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg
    43081 ccgtaaacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatca
    43141 cagccgtggt cagcggtaag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc
    43201 ggcagacggc ggtcgaacag ttccagaccc gcagcataac cggcgacgtc gcgacggtgg
    43261 ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct
    43321 ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cagtcgcttt cacttttttg
    43381 gtgataccgc agttggcgta gatgtccgca attttaccga cagaaaccac ctggccgtgt
    43441 ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caggtcgtga
    43501 cggttaccgg tacgctggaa gttaccggct ttgtcgccga taaacggacg agcgataaca
    43561 cgaccgatat tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag
    43621 agtttatcca gaccgaaagt ttcttcatga caggcaatct ggaacacgga gtcagcggag
    43681 gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagttgatc cagaatgacc
    43741 gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc gaccagttta
    43801 tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaacaga
    43861 accgggacac cggcgatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg
    43921 tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa
    43981 ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc
    44041 gggcctttac gaccgttatc agcttcgcct ttggcacaag cttcagcgat atgacccagg
    44101 gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag
    44161 tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa
    44221 atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc
    44281 ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg
    44341 ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaga
    44401 cgtgccatat cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg acggcgtccg
    44461 ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct
    44521 tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc
    44581 tcaacgaagt cggtcgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct
    44641 ttaccgttgt ccagcaccgc ctgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt
    44701 ttgccggaga tcagcatctc cacacacagc gccatcgtga catcaaacag acgcgggtta
    44761 cgatattcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg
    44821 gaggccagta cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta
    44881 gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg
    44941 ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgccag tttcttcgcc
    45001 aggatcgagg cagtgatcag cgggatggag tccacggttg cggtaatatc gcgggtcgcg
    45061 tagaaacgtt tatctgccgg agccagtgag ctggtctggc caataatcgc cacaccgacg
    45121 tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat
    45181 tccagtttgt cgagcgtacc gccagtatga ccgaggccac gaccggagat catcggaata
    45241 tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtgacatc gccgacgcca
    45301 ccggtggagt gtttatcaac aatcggtcca ttcagatgca ggcttttcca gtcgagaacg
    45361 gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg
    45421 aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaata
    45481 ccgttgataa agaaacgaat ttcttcatcg ctcagcgcat gaccatcacg ttttttacga
    45541 ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt
    45601 gccggatgcg tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag
    45661 cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcgccg
    45721 tggcccagcg ctttcagcag gcttgccagc aggctggaag cgccaaagcg gtagtgacgc
    45781 gcatcagccc agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct
    45841 tccgcagtac gcacgccgcc cgccggtttg aaaccaacgg ttttttctac gcccatatca
    45901 cggatcactt ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc cactttaccg
    45961 gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc
    46021 agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc
    46081 gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt acccgccatc
    46141 agcgcgcggt acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg
    46201 gtttctgcca gtgcgatgtc gatgtcgtcg ttaccgtgtg ggaagttggt taccgtagca
    46261 atacggattt ccggggtgcc ctgctctttc agtgttttgc gagcaatcgg gataaagcga
    46321 ggatagatac agatagcggc ggtattgccg accggagttt tggcctgatg acacagagca
    46381 attactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca
    46441 cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt
    46501 caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg atatacatca
    46561 caattaagga aatctactta caagtttgca tcactataat gcgatctggt tcaaataatt
    46621 cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga
    46681 aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca
    46741 tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga
    46801 gcagaagcgc cattttctgg cactggaagc agaaaacaat ctgccttacc cacagcttcc
    46861 tgccgaagcc cgccgcgcgc tggatgaagg tgtaatctgc gatatgtttg aaggtcatgc
    46921 gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga
    46981 atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct
    47041 ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt
    47101 gttgcaaccg tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt
    47161 ctggcgttac ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc
    47221 tgattcgccc attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc
    47281 gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc
    47341 gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat
    47401 tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg
    47461 tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga
    47521 tgacttcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc
    47581 gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga
    47641 agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc
    47701 ggttaacttg ctgtgtgaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc
    47761 aaatgaagtg ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt
    47821 gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggatca gttccgatat
    47881 cggcaccacg ccgggcgcgc gtttgccgta tggcgatgag ccagatccga tcacccatct
    47941 gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct
    48001 cgacgaaacc atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa
    48061 agccggaatg cgtgaattta ccgccaatgt cagcggtaac gatctggttc gcgttaccgg
    48121 ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac
    48181 cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt
    48241 gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg
    48301 acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact
    48361 gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc
    48421 cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtctgcgagc
    48481 aatgtgatac ctgcctgaag aggtgtccgc aacatgccac accaatggcg caatccatga
    48541 gcgtggagga ggtgcttagc catgtgcgca aagcggtgct gtttattgaa gggataacgg
    48601 taagcggcgg cgaagccacg acccaactgc cgtttgtggt ggcgctgttt actgctatca
    48661 aaaacgatcc gcaactgcgc cacctcacct gtctggtgga cagtaacggc atgttgagcg
    48721 aaaccggctg ggaaaaattg ctcccggtgt gcgacggcgc aatgctcgat ctcaaagcgt
    48781 gggggagcga atgtcatcag caattaaccg gacgcgataa tcagcagatt aagcgcagca
    48841 tctgtttgct ggcagagcgc ggtaagctgg cggaactgcg tttgctggta atccctggcc
    48901 aggtggatta tttgcaacac atcgaagaac tggcggcgtt tatcaaggga gtcggcgatg
    48961 ttccggtacg cctgaacgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg
    49021 cgagcgccac gccggaagac gttgagccgt tggctgatgc gttacgggag cgcggggtga
    49081 gccggttaat atttccggcg ctgtatttgt gacatgccgg atacggccta cggttcggca
    49141 cagacttgta ggcatgataa gacgcgtcaa gcgtcgcatc aggcattatg cgccgactgc
    49201 cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt aggcatgata
    49261 agacgcgaca agcgccgact gccggatgcg gcgtgaacgc cttatccggc ctacggttcg
    49321 gcacagactt gtaggcatga taagacgcga caagcgtcgc atcaggcatt gtgcgccaac
    49381 tgccggatgc ggcgtgaacg ccttatccgg cctacggttc ggcacaaacc tgtaggcatg
    49441 ataagacgcg ttaagcgtcg catcaggcat tatgcgccga ctgccggatg ctgcctacga
    49501 agggcaaact acccacgaac gttaaacacc gcatacgtgt tattaagcaa cacttcggca
    49561 atctcatccg cgggttccgg gcgtaactcg caaagcacgg caaacacacg ggcagcatgc
    49621 tccgggcggt taggctgccc ctgaaaaccg ttgagcggca tatccggcgc atcggtttcc
    49681 agcaacaacg atgccagcgg taattttgcg atgacatcgc gggttttact ggcgcgtgga
    49741 taggtgatag taccgcctac gccgattttg tagcccagca gtacaaaccg ttcagcctgt
    49801 tgctggctgc cagaaaatcc gtggacaaca ccagcacgcg gcaaatcgtg gcgtttaaga
    49861 tgcatcgcca gtttgtcgtg cgtgcgccgt gaatgcagga tcaccggcag atcgtagcgt
    49921 ttcgccagtt tcagttgttc gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg
    49981 ccgaagagat ccagaccgat ctcccccacc gccaccacct tcgccggacg cctttccagc
    50041 gcctgctgta gctgatcaag agacacatcg ctatgttttt ccaacatacc aggatgcaag
    50101 cccaatgcgg catacagcgg ttgataattt tccgctaacg cctgcacgcg ggcaaaattt
    50161 tccgcctctg tcgccggaac aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg
    50221 ctggcctctt catcgccact aaacggcggg aaatcaaaat ggcagtgggt gtcgataaaa
    50281 cggcaaatca agccagatcc tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcga
    50341 tcacttcggc aaccagcgta tcgttggcga ctggcgcagg cggaatgaca atcgattccg
    50401 gcgtcaccac tggcaccaga tggcgggtaa gcggcgcttt ttcagttaat agcttgccaa
    50461 ccgttgccag gaaataacga ccgcataaac gcccaagttt atagtcttcg cgcagcgccg
    50521 gaatccgact gccaagcgcg atactatgta atggcttcgg cggataaatt tcgaatatcc
    50581 gcagcttgcc cggtggtttc tcaataaatt gctgaatgtc acgatagctg gtttcatgat
    50641 gctgcaccag attgaccagc ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt
    50701 tgaaccactg cggcgtgtag tacatttgtg acggcacagt gcggatgacg accaacgttt
    50761 tagcgccctg ccttgccgcc tctttaaccg gaatcgcatc actgatcccg ccatccaggt
    50821 agttaatgcc ttccagcgac actccgctac gataaaagcc aggtatcgca ctggaggcgc
    50881 gaatcacatc cagccagttt tgtttggttg gtaaaaagta attcggcgcg tagtcatcct
    50941 gacgacaggc gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg gcggtatcca
    51001 tttgcaacgg catctggctt gcggtggcct ccaccagcca gtcgagatcg ataagatttc
    51061 ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag cgcatgatga
    51121 ctttgcgcgc gtaaccgggc tgattgcaga taaacgccga gaggttctgc gccccggcag
    51181 atgtgccgag ataaagatcg aaaggattaa actgcgcgcg cataaactca tccagcacgc
    51241 cagccgtgaa aattccacgc tgcccgccgc cttcgcacac cagagcaatt cgtccgggct
    51301 ggaacggcct tagcgacaac ggcgcaatat taccaagcgt tacaggtatt cgctgcccca
    51361 cctctgcctt cctgtttttg ttgtgaaaga atgacaaagt aacgcaattt attattcgct
    51421 aaaaccgcaa aagccagtcc gctggactgg ctttggataa aattttctta atggtctgaa
    51481 atctagggtc gttttcggcc catgaacaaa ctcaccagga acagaataat cccgacgaca
    51541 aagacaattt ttgctgcgcc tgcagcggta ccggccagac caccaaatcc aagtgcggcg
    51601 gcgattaacg cgataaccag aaatatgatg ccccaacgaa acataagcgt ctcctttacc
    51661 atagttaatg tcaccgcttg atatgagcgt gaaaatcact caatgcgata tttttagtgt
    51721 ggtgcacatc gcgcctcccg acaaagttcg ggaggacgaa ttacgacgaa ttacttagtt
    51781 ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact ttcagcacgg
    51841 tcactttgtg cctgagaatc tacggtaccg gagagctgaa ccacgccatc ggtggtttca
    51901 actttcacat gacgggaagg gacgatatcg tccgccagca gtttggcttt gatttcactg
    51961 gtggtggcgg tgtcacccgc gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc
    52021 agtttgtcgc tgacagaggt caccccttca acgcctttcg ccactttcac tgcctcttcg
    52081 gcctgggcct ggctttcaac gaaaccgctc agggtcacga ctttttgatc ggttttgaca
    52141 gagatatcgg tgctcttgat gttgtcatga tccaccaggg ccgccttcac tttcgcggtg
    52201 atggcgctgt catccatgaa attaccgact ttattcatag agctatcgac tttttgccct
    52261 gcgctttcat tggtagtctg tgcgttgttt tccgcgtagg cagagccggt cgcgacggca
    52321 gaggtcaaca ttacagccag cagagttttc gaaatcttca gtcttgtcat agtcatcgat
    52381 ttattcctgt atgtttgctc gtaatttgag cctggcaaca cgaggttgca ttgctgaata
    52441 gggagagact tcaccctcta cagaagtcaa tggtcgccat cacaaaagcg atgagtgatg
    52501 aataacgacc attacagcct ctgaatcagt tattaatatc ggtagaatga caatcgacgg
    52561 ctttagatac tgatatctac gcattgaacg gtatttaacg ccgtcagaaa tgtcatcact
    52621 ttgttaaata tagatcacaa ttttgaaacc gctcgggata tcagcgaaaa cataagcaaa
    52681 agtgaatgtt ttaagaacat tccgtaagcg gctaataagg aagggaaatt gacagggcgc
    52741 agcggctacc gcgccctgga ggcaagaatt aatgctcgcg ggtctggtgg aactgaacgt
    52801 ccggataacg ttcctgtgcc aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt
    52861 tatcgccgcc atcaagcgcc agttggcttt cgttcttacg cttgaactct tcgaatttct
    52921 tcgcgtctgc acattctacc cagcgggcag tggcaacgtt gactgactca tacactgctt
    52981 caacgttgta ttcgctcttc aggcgcgcta ccaccacatc aaactgcagc acaccaactg
    53041 caccaacgat cagatcgttg ttggagattg gacggaacac ctgcaccgcg ccctcttcgg
    53101 aaagctgtac cagccctttg agcagctgtt tttgcttcag cggatctttc aggcggatac
    53161 gacggaacag ttctggtgcg aagttcggaa taccggtgaa cttcatcatc tcaccctggg
    53221 taaaggtgtc gccgatctga atggtgccgt ggttgtgcag gccgaggata tcgcccggat
    53281 acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt cagcgcgtcg gagatcacca
    53341 catctttcgc agtgcgcacc tggcgcagtt tcatgccttt ttcatattta ccggacacca
    53401 cacgcataaa cgccacgcgg tcgcggtgtt tcgggtccat gttggcctga attttaaata
    53461 cgaagccggt aaatttatct tcgctcgctt ctacggtacg ggtatcggtc tgacgcggca
    53521 tcggcgcagg cgcccactcc accaggccat ccagcatatg atcaacgccg aagttaccca
    53581 gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag gaacagctct ttgtcgaact
    53641 cgttagacgc gcctttaacc agttccagtt cgtcacgcag ctgctgtgcc agatcttcac
    53701 caaccgcagc atcgagatcc gggttattca gccctttaac aatgcggact tcctgaatgg
    53761 tgtgcccttt accgctctga tagagatagg tttcatcttt ataaaggtgg taaacgcctt
    53821 taaacagctt gccgcagcca attggccagg tgatcggtgc gcagccaatt ttcagctcgt
    53881 tctcaacttc atcgagcaat tccatcgggt cgcggatatc acggtcaagt ttgttcataa
    53941 aggtgaggat cggcgtgtcg cgcagacggg taacttccat cagcttacgg gtacgatctt
    54001 caacaccttt tgcggcgtcg ataaccatca ggcagcagtc caccgccgtc agggtacgat
    54061 aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag caggttaacc aggcaatcgt
    54121 gatacggaaa ctgcatcaca gacgtagtaa tggagatccc acgctgcttt tccatctcca
    54181 tccagtccga cttagcgtgc tggttggaac cacggccttt tactgtaccg gcagtctgaa
    54241 tggcctgtcc gaacagcagc accttctcgg tgatggtagt cttaccggcg tccgggtgag
    54301 aaataattgc aaaggttctt cttttcgcta cctctgcggc caaaaggctt gtcataattg
    54361 ttgtctcttt gattatgtac aatcatatgt acaaagaaaa attcatgccg ctgattgtac
    54421 attttggagg tatcagagca tagcagaaca tgatcagtag gtgtatttgt agccaaagaa
    54481 catttgtaca tgcagcaatc tttgttctat tgacactgtt tattcataca gttaaaaata
    54541 acactgtata caaacacagt gtagagggat ttttatgcgt attgagatct gcatagccaa
    54601 agagaaaatg actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaactcg
    54661 acgcatcagt aagcgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg
    54721 cctttctgtt acgcgcaccg ccgataaaga ttcagctaaa acttttgttc aggaaactct
    54781 gaaagatacc tgggagtctg ctgacgagtg gtttgttcac taattaacac gtaaaatcgg
    54841 taacggctgg aaatcattca atactcgcac tatcggaagt tcaccagcca accgcagcac
    54901 gttcttgcat acgacgtgtc tgcggttttt caaccattca gatacagcac tctcatcaca
    54961 ttcattcttc cgcattaaag tatatatgac catatcacct taatattttt cttcttcaga
    55021 taaaaattgt tatctatgta tacttttaaa ccgaatccgt gtagagtctc tacctaagtt
    55081 agttcgcagc taccccttca gtttgcgcca ttaac
//
LOCUS       DAZGCQ010000020        53959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000020 DAZGCQ010000000
VERSION     DAZGCQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.020"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..867
                     /gene="rhaD"
                     /locus_tag="ACHK07_001300"
     CDS             43..867
                     /gene="rhaD"
                     /locus_tag="ACHK07_001300"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO8101659.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAHEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            877..1191
                     /gene="rhaM"
                     /locus_tag="ACHK07_001301"
     CDS             877..1191
                     /gene="rhaM"
                     /locus_tag="ACHK07_001301"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO8101660.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(1232..2626)
                     /locus_tag="ACHK07_001302"
     CDS             complement(1232..2626)
                     /locus_tag="ACHK07_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO8101661.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            3104..3550
                     /gene="frvA"
                     /locus_tag="ACHK07_001303"
     CDS             3104..3550
                     /gene="frvA"
                     /locus_tag="ACHK07_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO8101662.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            3561..5012
                     /gene="frvB"
                     /locus_tag="ACHK07_001304"
     CDS             3561..5012
                     /gene="frvB"
                     /locus_tag="ACHK07_001304"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO8101663.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            5002..6072
                     /gene="frvX"
                     /locus_tag="ACHK07_001305"
     CDS             5002..6072
                     /gene="frvX"
                     /locus_tag="ACHK07_001305"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8101664.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLMLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            6072..7820
                     /gene="frvR"
                     /locus_tag="ACHK07_001306"
     CDS             6072..7820
                     /gene="frvR"
                     /locus_tag="ACHK07_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO8101665.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(7922..8977)
                     /gene="yiiG"
                     /locus_tag="ACHK07_001307"
     CDS             complement(7922..8977)
                     /gene="yiiG"
                     /locus_tag="ACHK07_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO8101666.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNRLR"
     gene            complement(9130..9963)
                     /gene="fdhD"
                     /locus_tag="ACHK07_001308"
     CDS             complement(9130..9963)
                     /gene="fdhD"
                     /locus_tag="ACHK07_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO8101667.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            10157..13207
                     /gene="fdnG"
                     /locus_tag="ACHK07_001309"
     CDS             10157..13207
                     /gene="fdnG"
                     /locus_tag="ACHK07_001309"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:10742..10744,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8101668.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            13220..14122
                     /gene="fdoH"
                     /locus_tag="ACHK07_001310"
     CDS             13220..14122
                     /gene="fdoH"
                     /locus_tag="ACHK07_001310"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO8101669.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            14119..14754
                     /gene="fdoI"
                     /locus_tag="ACHK07_001311"
     CDS             14119..14754
                     /gene="fdoI"
                     /locus_tag="ACHK07_001311"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8101670.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            14751..15680
                     /gene="fdhE"
                     /locus_tag="ACHK07_001312"
     CDS             14751..15680
                     /gene="fdhE"
                     /locus_tag="ACHK07_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO8101671.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(16010..16252)
                     /gene="yiiF"
                     /locus_tag="ACHK07_001313"
     CDS             complement(16010..16252)
                     /gene="yiiF"
                     /locus_tag="ACHK07_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO8101672.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(16469..16687)
                     /gene="yiiE"
                     /locus_tag="ACHK07_001314"
     CDS             complement(16469..16687)
                     /gene="yiiE"
                     /locus_tag="ACHK07_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO8101673.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(17381..18295)
                     /locus_tag="ACHK07_001315"
     CDS             complement(17381..18295)
                     /locus_tag="ACHK07_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8101674.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(18308..19195)
                     /locus_tag="ACHK07_001316"
                     /pseudo
     CDS             complement(18308..19195)
                     /locus_tag="ACHK07_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19605..20546)
                     /gene="fabY"
                     /locus_tag="ACHK07_001317"
                     /gene_synonym="yiiD"
     CDS             complement(19605..20546)
                     /gene="fabY"
                     /locus_tag="ACHK07_001317"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO8101675.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(20591..21028)
                     /gene="dtd"
                     /locus_tag="ACHK07_001318"
     CDS             complement(20591..21028)
                     /gene="dtd"
                     /locus_tag="ACHK07_001318"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO8101676.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(21025..21897)
                     /gene="yihY"
                     /locus_tag="ACHK07_001319"
     CDS             complement(21025..21897)
                     /gene="yihY"
                     /locus_tag="ACHK07_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO8101677.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(21891..22490)
                     /gene="yihX"
                     /locus_tag="ACHK07_001320"
     CDS             complement(21891..22490)
                     /gene="yihX"
                     /locus_tag="ACHK07_001320"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO8101678.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TAFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(22631..23518)
                     /locus_tag="ACHK07_001321"
     CDS             complement(22631..23518)
                     /locus_tag="ACHK07_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8101679.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(23508..24857)
                     /locus_tag="ACHK07_001322"
     CDS             complement(23508..24857)
                     /locus_tag="ACHK07_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101680.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(24956..26389)
                     /locus_tag="ACHK07_001323"
     CDS             complement(24956..26389)
                     /locus_tag="ACHK07_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101681.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNTLCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLIHWEPLGANVDAEDAEVLAELKK"
     gene            complement(26459..27469)
                     /locus_tag="ACHK07_001324"
     CDS             complement(26459..27469)
                     /locus_tag="ACHK07_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO8101682.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(27502..28389)
                     /locus_tag="ACHK07_001325"
     CDS             complement(27502..28389)
                     /locus_tag="ACHK07_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO8101683.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQIMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(28414..29292)
                     /gene="yihU"
                     /locus_tag="ACHK07_001326"
     CDS             complement(28414..29292)
                     /gene="yihU"
                     /locus_tag="ACHK07_001326"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO8101684.1"
                     /translation="MATIAFLGLGQMGSPMANNLLHKGHSLQVFDVNALAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            29465..30361
                     /locus_tag="ACHK07_001327"
     CDS             29465..30361
                     /locus_tag="ACHK07_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8101685.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIARLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            30401..31189
                     /locus_tag="ACHK07_001328"
     CDS             30401..31189
                     /locus_tag="ACHK07_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8101686.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(31300..32565)
                     /gene="yihN"
                     /locus_tag="ACHK07_001329"
     CDS             complement(31300..32565)
                     /gene="yihN"
                     /locus_tag="ACHK07_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101687.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVKLKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(32667..33647)
                     /gene="yihM"
                     /locus_tag="ACHK07_001330"
     CDS             complement(32667..33647)
                     /gene="yihM"
                     /locus_tag="ACHK07_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO8101688.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(33655..34365)
                     /gene="yihL"
                     /locus_tag="ACHK07_001331"
     CDS             complement(33655..34365)
                     /gene="yihL"
                     /locus_tag="ACHK07_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8101689.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(34582..36405)
                     /gene="typA"
                     /locus_tag="ACHK07_001332"
     CDS             complement(34582..36405)
                     /gene="typA"
                     /locus_tag="ACHK07_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO8101690.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            36778..38187
                     /gene="glnA"
                     /locus_tag="ACHK07_001333"
     CDS             36778..38187
                     /gene="glnA"
                     /locus_tag="ACHK07_001333"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO8101691.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            38361..39410
                     /gene="glnL"
                     /locus_tag="ACHK07_001334"
     CDS             38361..39410
                     /gene="glnL"
                     /locus_tag="ACHK07_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO8101692.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            39422..40831
                     /gene="glnG"
                     /locus_tag="ACHK07_001335"
     CDS             39422..40831
                     /gene="glnG"
                     /locus_tag="ACHK07_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO8101693.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            40943..41053
                     /gene="yshB"
                     /locus_tag="ACHK07_001336"
     CDS             40943..41053
                     /gene="yshB"
                     /locus_tag="ACHK07_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO8101694.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(41243..42616)
                     /gene="hemN"
                     /locus_tag="ACHK07_001337"
     CDS             complement(41243..42616)
                     /gene="hemN"
                     /locus_tag="ACHK07_001337"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO8101695.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(42805..43311)
                     /gene="yihI"
                     /locus_tag="ACHK07_001338"
     CDS             complement(42805..43311)
                     /gene="yihI"
                     /locus_tag="ACHK07_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO8101696.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(43430..43596)
                     /locus_tag="ACHK07_001339"
                     /pseudo
     CDS             complement(43430..43596)
                     /locus_tag="ACHK07_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43893..44525
                     /gene="yihA"
                     /locus_tag="ACHK07_001340"
     CDS             43893..44525
                     /gene="yihA"
                     /locus_tag="ACHK07_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO8101697.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(44907..47693)
                     /gene="polA"
                     /locus_tag="ACHK07_001341"
     CDS             complement(44907..47693)
                     /gene="polA"
                     /locus_tag="ACHK07_001341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO8101698.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIESGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALDEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAYEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LKYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            48057..48989
                     /gene="yihG"
                     /locus_tag="ACHK07_001342"
     CDS             48057..48989
                     /gene="yihG"
                     /locus_tag="ACHK07_001342"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO8101699.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRHDK"
     gene            complement(49030..50460)
                     /gene="yihF"
                     /locus_tag="ACHK07_001343"
     CDS             complement(49030..50460)
                     /gene="yihF"
                     /locus_tag="ACHK07_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8101700.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAS
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEMNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLHY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(50615..51241)
                     /gene="dsbA"
                     /locus_tag="ACHK07_001344"
     CDS             complement(50615..51241)
                     /gene="dsbA"
                     /locus_tag="ACHK07_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO8101701.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(51258..52244)
                     /gene="srkA"
                     /locus_tag="ACHK07_001345"
     CDS             complement(51258..52244)
                     /gene="srkA"
                     /locus_tag="ACHK07_001345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO8101702.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFNHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(52321..52590)
                     /gene="yihD"
                     /locus_tag="ACHK07_001346"
     CDS             complement(52321..52590)
                     /gene="yihD"
                     /locus_tag="ACHK07_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO8101703.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            52660..53244
                     /gene="mobA"
                     /locus_tag="ACHK07_001347"
     CDS             52660..53244
                     /gene="mobA"
                     /locus_tag="ACHK07_001347"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO8101704.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            53226..53753
                     /gene="mobB"
                     /locus_tag="ACHK07_001348"
     CDS             53226..53753
                     /gene="mobB"
                     /locus_tag="ACHK07_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO8101705.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(53855..>53959)
                     /gene="rrf"
                     /locus_tag="ACHK07_001349"
     rRNA            complement(53855..>53959)
                     /gene="rrf"
                     /locus_tag="ACHK07_001349"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        13623 a        13730 c        12939 g        13667 t
ORIGIN      
        1 ggtcgtgcat ccgacaacac cgaatttaca ggaacacaga acatgcaaaa tattacccag
       61 tcctggtttg tccagggaat gatcaaagcc accaccgacg cctggctaaa aggctgggac
      121 gagcgcaacg gcggcaacct gacgctgcgc ctggatgacg ccgatatcgc gctttatcac
      181 gacaacttcc acccacaacc gcgctatatt ccgctcagcc agcccatgcc tttactggca
      241 aatacgccgt ttattgtcac cggctccggc aaattcttcc gtaacgtcca gcttgatcct
      301 gcagctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca tattctctgg
      361 gggttaacca acgaagccgt tccaacttct gaacttccgg ctcactttct ctcccactgc
      421 gagcgtatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca tgccaccaac
      481 ctgatcgccc tcacctatgt actggaaaac gacaccgcgg tcttcactcg ccaactgtgg
      541 gaaggcagca ccgagtgtct ggtggtattc ccggatggcg ttggcattct gccgtggatg
      601 gtgcccggca cggacgaaat cggccaggca actgcccacg agatgcaaaa acattcgctg
      661 gtgttgtggc ccttccacgg tgtgttcggt tccggaccga cgctggatga aacattcggt
      721 ttaatcgaca ccgcagaaaa atcagcggaa gtgttagtga aggtgtattc gatgggcggc
      781 atgaaacaga ccatcagccg tgaagagttg atagcgctcg gcaagcgctt cggcgttaca
      841 ccactagcca gtgcgctggc gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg
      901 caggttaacc ccgacgccca cgaagagtat cagcgtcggc ataaccccat ctggccggaa
      961 ctggaagcag tgttgaaatc tcacggtgcg cataactacg ccatttacct cgacaaagcg
     1021 cgtaatctgc tgtttgccat ggtagagatt gaatctgaag aacggtggaa tgcggtagcc
     1081 agcactgaaa tctgccaacg ttggtggaaa tatatgaccg acgttatgcc cgctaacccg
     1141 gataacagcc cggtgagtag cgagctgcaa gaagtgtttt acctgcctta atgagttgcg
     1201 gtgccggaaa atatccagca ccgcgacttc atcagaagaa gtatttaaag cgaacgcgtg
     1261 cgccgtaacg gtcatcagta tcatcaccgt gttttttatc gccgtcgcta tccagaatgg
     1321 agtaatacgt cccgaggtaa atattaaagt tctccatatc catcacatta gcgaactgat
     1381 aagacgcatg aatggtgtga atggtgtacg tcccttcatc atcaatccag cagtcgttat
     1441 cacaaactac accgctgaat tcatcaatct tattgtgcgc gtagatataa ccgagctcga
     1501 aacgtttcca cagcgcgtta atcccggcag tgaaatcttt ctcattatta gcatctaaat
     1561 aggcggtatt aagattaacc acaatgccat tgtccggatc cgttttcagg ccgttccagg
     1621 tcatgctcat accatagccg gtacgatcgg actgatcgac aaaattaccc ttgctatcgg
     1681 tataaccata agcattattt accacgttcg cttccatcgc tgcggaaacg gtgaattctt
     1741 ccgtcggcga ccaggcaatt actgggcgca gataagcaac atttttctgc tgctccatat
     1801 cgcgtccatg ataattaccg tcattatata aagatgttcc gtcttcaagt aacgtgttta
     1861 attcaaaata ccagttatcg agttgtttgc tgacgaggaa attaccgcca gcgttagagc
     1921 gcccgcggcc ctctttcatc atatagatat aaccgctgcc atcgtcataa agatcgttcg
     1981 cagtattacc ggaatgttca acaaaggtat cctgattcag cgggaacata tcgtaggctt
     2041 caaaacggcc tactttgacc ttccagtcgt tttctttgcc aaagaagaat accgcatcat
     2101 cgatatttac tgaaccgtgc atatcaccca gcggttgtgc ggagaacccg gcgaaataac
     2161 cgttgtccat ctttcgcata ccgtcaaaac ctaacagaat acgaccattg agattccact
     2221 gttcattagt gggatcgcta ttcacatcag cgttggtcat tgccagcagg ccatgattac
     2281 tttccgcatc catattaaat tctacgtcgc cgtagatttt taaatcgcca taacctgaaa
     2341 gcaaaagatt aggtgaggtg gcatttttct ttgcagattc cgccgttgtc tgtccattta
     2401 ccggcgtatt gccctgggcg gccttaattt cgcggatctc agcggcttgt tgtgtctgca
     2461 gctgttttac ctgcccttct gcttttgctg cccgcatttc tgccgcttcc aggcgttctt
     2521 ccagcaatgc catccgttgt tccagcgtta aatgctgtgt ttttgccata ccggtactac
     2581 atgccaataa taaaccgatg gctaaagata atgtcgattt tttcattatt tacgttccct
     2641 gaaataaagg aggccctacg ctttccaaaa ggaatacgta tatagagaaa tataaagtgt
     2701 gcactttcga atttttaatc caacttcagc aggccgatgg atagtatccc gccgcataat
     2761 atgagtaaat ccgctgattt cctggaacgt gaggaatata acaatagtgc cgtttttaac
     2821 taaatatcgt aaaaaatatt agatatgatt caagctgact cataagccac aaatataaat
     2881 aacgtttaga caaattatct gagacattta aacattcaca aaatcattaa ataataactc
     2941 tttattcaca agatttattt tacctactaa tatgtgcctt acgccgcaaa atttatcctt
     3001 cactctgctt tatattgtca cgacttcacg acatatccgc gctcatcaca aacccgcccc
     3061 gctattacaa ttaactgaaa tattttttca tggagtccga ttaatggcag ctcttactgc
     3121 aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccattctga agcagttagc
     3181 gacaatggcg ttacaaaacg gttttatcac cgactcacac cagttcctgc aaaccctgct
     3241 gctgcgcgaa aaaatgcact ccaccggttt tggttctggt gtcgccgtgc cgcacggtaa
     3301 aagcgcctgc gttaaacaac cgttcgtgtt attcgcccgc aaagcgcagg ctattgactg
     3361 gaaagccagc gacggcgaag acgtcaattg ctggatctgc ctcggcgtgc cgcaaagtgg
     3421 cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc aaaattattc accaggattt
     3481 tattcaccaa ctgcaacagg gcgacgccga ccaagtgctt gccttgttaa atcaaaccct
     3541 cagctcataa ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc accaactgcc
     3601 ccgccgggat cgctcacacc tacatggtgg cggaagccct ggaacagaaa gcccgttctc
     3661 tcggccatac cataaaagtg gaaactcaag ggtccagcgg cgttgaaaac cgcttatcca
     3721 gcgaagagat tgccgctgcc gattacgtga ttctcgccac cgggcgtggc ctgagcggtg
     3781 acgatcgcgc acggtttgcc gggaaaaaag tttatgagat tgccatctcc caggcgctga
     3841 aaaatatcga tcagattttc agtgaattac cgacaaattc gcagcttttt gccgcagata
     3901 gcggcgtgaa gctaggcaaa caggaagtac aaagcggcag cgtgatgagc cacctgatgg
     3961 cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg gcgcttgcca
     4021 acatgctggt gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg ccgtcgttta
     4081 cctgggtagt tgaatccatc ggttaccttg gcttcacctt tatgattccc atcatgggcg
     4141 cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt ctggtttgct
     4201 atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc ctcggcgcgg
     4261 tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgtaaagtc cgtctcggca
     4321 aagcgctaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg ctggttttcg
     4381 gcgtgctcac ttactacgtt atcggaccgg tgatgtccga cctcatgggc gggctactgc
     4441 acttcctcaa taccattcct ccatcaatga agtttgcggc agcgtttctg gtcggtgcga
     4501 tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc tgcttctcac
     4561 tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg ctgatgccgc
     4621 ccgtcgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga caggaaaaag
     4681 aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg gctattcctt
     4741 acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggc ggtattaccg
     4801 gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc tggtttgggt atctttgacc
     4861 cgctgatcgg cctgatgtcg ccggtgggtt cgttttatct ggtgctggcg attggcctcg
     4921 cgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc aaagcgaaag
     4981 ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg cgaagccagc
     5041 gccgtcagcg gcgatgaaca ggaagttcgc gacattctga taaacacgct ggaaccttgc
     5101 gttaatgaga tcacctttga tggtctgggc agctttgttg cccgtaaggg aaataaaggt
     5161 ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac ccacatcgac
     5221 gagagcggtt ttctgcgctt taccaccatt ggcggctggt ggaatcagtc gatgctcaac
     5281 caccgggtaa caatacgcac acacaaggga gtgaaaatcc ctggtgtgat tggttccgta
     5341 gcgcctcatg cgttaacgga aaagcaaaag caacaaccgc tgtcatttga tgagatgttc
     5401 attgatattg gcgcgaacag tcgcgaagaa gcgaaaaaac gcggcgtgga aattggtaat
     5461 tttattagcc cggaggccaa ttttgcctgc tggggcgaag ataaagtagt cggcaaggcg
     5521 ctggataacc gcatcggctg cgcgatgatg gcggagctac tacagacagt aaataaccca
     5581 gaaattacgc tgtacggcgt cggcagcgtg gaagaagaag ttgggctacg cggggcacaa
     5641 acctcggctg aacacactaa accggatgtg gtgatcgtgc tggataccgc cgtcgcgggc
     5701 gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg gccggggctg
     5761 atgctgtttg acaagcgcta cttccccaac cagaaactgg tagcagcgtt aaaaaactgt
     5821 gccgcacata acggtttacc gctgcaattt tccaccatga aaaccggagc gacggatggc
     5881 gggcgctaca acgtgatggg cggcgggcgt ccggttgtcg cgctgtgtct gccaactcgt
     5941 tatctgcacg ccaacagcgg catgatttca aaagccgatt acgatgctct gctcatgctg
     6001 atacggggtt ttctgacgac cttaaccgcg gagaaagtca acgcgtttag tcagttccgt
     6061 caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg gagcaacagc
     6121 cgcgcacgcc tggcgagctg gcgcaacaga ctggcgtttc aggcaggacc atcctgcgtg
     6181 atattgacta tctcaacttc acccttaacg gcaaagcccg cattttcgcc agtggcagtg
     6241 cgggctatca gctggaaatc ttcgagcgcc gcagtttttt tcagttgctg caaaagcacg
     6301 ataacgacga tcggctgctg gcgctgttat tactgaatac tttcactccc cgtgcgcaac
     6361 tcgcctcggc gcttaatttg ccagaaacgt gggtagcaga gcgtctgccc cgcctgaaac
     6421 agcgttatga acgtacttgc tgcctggcca gtcgccccgg tttgggccat ttcattgatg
     6481 aaacagaaga gaaacgcgtt atcttgctgg cgaacttgct acgcaaagat ccgtttttaa
     6541 taccgctggc gggcgtaaca cgagataacc ttcagcattt atccacggtc tgcgacaacc
     6601 aacaccgctg gccgctgatg cagggtgatt atctctccag cctgattctg gcgatttacg
     6661 ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc cggtaacgag ataaaacaaa
     6721 tcgttgaaca gagcggcctg tttcttggtg ataatgctgt aagaaccctg acgggtttga
     6781 tagagaaaca gcatcagcaa gcgcaggtaa tttctgccga tcatgtgctg gggttgctgc
     6841 aaagggtgcc aggcatcgcg tcattgaata ttattgatac gcagttggtt gagaatatta
     6901 ccgggcattt attacgttgc cttgccgcac cggtgtggat tgccgagcac cgccaaagca
     6961 gcatgaataa cctgaaagcc gcctggcctg cggcctttga tatgagtctg cactttatta
     7021 cgctactgcg agaacagctc gatattcctc ttttcgacag cgatctgatc ggtttgtatt
     7081 ttgcctgtgc gctggaacga catcaaaacg aacgccagcc gatcattttg ctctcggacc
     7141 agaacgcgat tgccaccatt aatcagctcg ccattgagcg cgatgtttta cattgtcggg
     7201 tgattattgc ccgtagctta agcgagcttg ttgccattcg cgaagagatt gagccgttat
     7261 tgatcattaa caacagccat tatttactgg atgacgcggt aaataattac atcaccgtaa
     7321 aaaatatcat tacggctgcc ggtatcgaac aaataaaaca ttttctggcg acggcattta
     7381 ttcgccagca accggagcgt tttttctctg cccctggaag ttttcattat tcgaatgtac
     7441 gcggtgaaag ctggcaacat attacccggc aaatttgtgc gcaattagtc gcacaacacc
     7501 atattaccgc cgatgaagcg caacggatca tcgcccgcga aggcgaaggt gaaaacctga
     7561 ttgttaatcg cctcgccatc ccacattgct ggagcgaaca ggagcgacgt tttcgtggat
     7621 tttttattac cctcgcccaa ccagttgagg tgaataacga agtcattaac catgtcttga
     7681 tcgcctgcgc cgccgccgat gcgcgtcatg agctaaaaat atttagctat ctggcaagcg
     7741 tattgtgtca gcatccggcg gagactattg tcggactaac ggggtatgaa gggtttatgg
     7801 cgttacttcg ccaggggtga atgatgtcgg atgcaccgct aacacggctt aacgacgttt
     7861 aggtcggata aggcgtttac gccgcatccg accttgtatt tctcagcaaa aacaaaaacg
     7921 tttaacgcag gcgattataa tcatcgacca tttcgttgta ctcgcgtaat gctctcggga
     7981 aagagtcttc gaccatccag cttgagtttg cgtcctgaag ttgctcttta tcccagtcag
     8041 agtacgggac tttatctctg atgcggcgaa cgtatttttt agcctcgagt tgatactggc
     8101 ctgccgaatt aataaacgag aaattcatgc cgcctttatc cgcttctttg gcctgcgcca
     8161 ccagcgtttc cagttcagac actttcttca ttgccgcttc tgcatcaaac tcatcttgcg
     8221 atatcaggtt attaatttgt ttcgctgaaa tcatgactgc cagagagtag tagtggaatg
     8281 ttttcccttc tctttcttca atatttttca gctccgcaag ctggcgctta tcattaattt
     8341 cctgaatcgc cgcatgataa gattcggcta caggttcaaa ggcttccaga ttttttaaga
     8401 atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt
     8461 aatatttatc catttcgtta atggtattac ccaacgccac ggcagcatcg atataactca
     8521 ccgctacgcc atcaataggt tgcagcgcag gcgttaacgc cacagcactt tttacgcctt
     8581 tttcacactc agcgaggttg gattcactaa tgccgtagat gccataaaca gtacgctctt
     8641 caccggtagg cccctgttta aaatctttca gccagtcagc ataacgcgcc agactgcgct
     8701 gtaccgggat ctgtaacttg ttgtagcact tgatatagac atccattttt tcgtcgaaaa
     8761 ccgcttgttc atcgaccacc ggttgggcag gcgtttcagg tttagcttct gcttttgcaa
     8821 cgggagcttc agtaggtttc gcttcaggag ctggtgcggc aggttgacta ttggcaggcg
     8881 ggagcgtttc tgtttcggct tttttatcat cacaacccgt cagaccgagg gccatgatgg
     8941 cgactataat agcggaagat aataaattcc ttttcattta attgccttat aaatatatag
     9001 ataagagagt gaacataata aatgaaaaaa gaaaacgcca ctacacgcgt tttatttata
     9061 aataaaacaa taaaattcac gaaatgaata agtcctgcaa tttaatatat atcgcaggac
     9121 ttattttaat taattgctca aacgctgcgg atgggtataa accgttgccc gacccggttt
     9181 acaaaaacct accagtgtca aattacagcg ctcagccact tctacagcaa gcgtggtcgc
     9241 ggcagacacc gcaaacaaaa tctctacgcc gcacatcgcc gacttttgca ccatttcata
     9301 actggcacga ctggaaacca gtaccgcacc ttgctgccag ctttcccctt cttgtgaccg
     9361 gcggcctaac agtttgtcca gcgctacatg gcgacccacg tcttcatgcc cgccgaccag
     9421 ttcgccagat ggcaacatcc aggcagcggc gtgagtacaa ccagtcagtt gtcccactgg
     9481 ctgaaaatcg ttgagatgac gtaatgcatc atccagtttg ttgagatcaa acgtctgggt
     9541 gaacggtagc ggctgcaccg gttttccgat gtcattaagt tgctccacgc cgcatacgcc
     9601 acagcccgta cgtcccgcca gcgcccggcg gcgctccttc aaccccataa agcggcggct
     9661 ggaaagctca atttgtactt caagaccatt acaggaagga acgacatcca tgccgaagat
     9721 atcgcgcgga ctttctatta tcccctcgga aagcgaaaaa ccgagcgcaa agtactcaag
     9781 gtctttgggc gacgccatca tcaccacatg cgaaatgccg ttgtagacca gcgcaacggg
     9841 aacttcttcc gccacctcat ctaaccgtgg gtgttgtaaa tcatcacgcc gccataactc
     9901 aatttgacga acacctgtga catttgtcac attttcgatt tctttttgct gtgttttttt
     9961 cactttctat taaccggata agaacacaca caccaacatt atggtattct gttacaaacc
    10021 cttcctggat ggagggaaat tgagccaatt ctggaccttt gcggccactt ccgcaaagaa
    10081 aaataactcc cactccctgc acacgcagca agcgaatgta aatgggacgt gacaatgtcg
    10141 aaacaaggag caatccatgc aggtcagcag aaggcagttc tttaagatct gcgctggcgg
    10201 tatggcaggc accacggcag cggcactggg ttttgcaccc agcgtagcac tcgcggaaac
    10261 ccggcagtat aaactgctgc gcacccgcga aacccgtaat acctgcacct attgttccgt
    10321 aggctgtggg ctgttgatgt acagcctcgg tgacggagca aaaaacgcca aagcatctat
    10381 cttccatatc gaaggcgatc cggatcaccc ggtcaaccgc ggtgcacttt gtccgaaagg
    10441 cgctggcctg gtggatttca tccactccga aagccgtctg aagtttccgg aataccgtgc
    10501 gccaggttct gataaatggc agcaaatcag ttgggaagag gcgtttgatc gcatcgccaa
    10561 actgatgaaa gaagaccgcg atgctaacta cattgcgcaa aacgccgaag gcgtgactgt
    10621 taaccgctgg ctctccaccg ggatgctgtg tgcttccgcg tcgagtaacg aaaccggcta
    10681 tttaacgcaa aaattctccc gcgcgctggg tatgctcgcg gtcgacaacc aggcgcgtgt
    10741 ctgacacgga ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa
    10801 ccactgggtc gacatcaaga acgccaacct cgtcgtggtg atgggcggta acgccgctga
    10861 agctcacccg gtcgggttcc gctgggcgat ggaagccaaa attcacaacg gcgctaagct
    10921 gattgtgatc gatcctcgct ttacgcgtac ggcggcggta gctgactact atgcccctat
    10981 tcgttccggt actgacattg ctttcctgtc aggcgtattg ctgtacctgc tgaacaatga
    11041 aaaattcaac cgcgaataca ctgaagccta taccaacgcc agcctgatcg tgcgtgagga
    11101 ttacggcttt gaagatggcc tgttcaccgg ctacgacgcg gaaaaacgca agtacgataa
    11161 atcctcctgg acttatgaac tggacgaaaa cggcttcgcc aaacgcgata ccacgctgca
    11221 acatccgcgc tgcgtgtgga acttgctgaa acagcacgtt tcccgctaca cgccagatgt
    11281 ggttgaaaac atctgtggta cgccaaaaga cgcgttcctg aaagtctgcg aatacatcgc
    11341 agaaaccagt gctcacgata aaactgcctc gttcctgtat gccctcggct ggacgcaaca
    11401 ctccgttggt gcgcaaaaca ttcgtacgat ggcgatgatc cagttgctgc tcggtaacat
    11461 ggggatggct ggcggcggcg ttaacgccct gcgcggccac tccaatattc aggggctgac
    11521 ggacctgggg ctgctgtcgc agagcctgcc gggttacatg acgctgccaa gcgagaagca
    11581 gaccgatctg caaacctacc ttaccgccaa cacgccaaaa ccgctgctgg aaggccaggt
    11641 aaactactgg ggcaactacc cgaaattctt cgtctctatg atgaaggcct tctttggtga
    11701 taaagcgacg gcggaaaata gctggggctt tgactggttg ccgaagtggg ataaaggcta
    11761 cgacgtcctg caatacttcg agatgatgaa agagggcaag gtcaatggct atatctgcca
    11821 gggctttaac cctgttgcct cattcccgaa caaaaacaaa gtgatcggtt gtctgtcgaa
    11881 actgaagttc ctcgtcacca tcgacccgct gaacactgaa acctctaact tctggcagaa
    11941 ccacggtgag ctgaacgaag ttgactcgtc gaagatccag accgaagtgt tccgtctgcc
    12001 atcgacctgc ttcgcagaag agaacggttc aatcgttaac tcaggtcgct ggttgcagtg
    12061 gcactggaaa ggtgcggacg ccccggggat tgcgctgact gacggcgaga tcctctccgg
    12121 tatcttcctg cgcttgcgca agatgtatgc cgaacagggt ggcgcgaacc cggaccaggt
    12181 gctgaacatg acctggaact acgccattcc gcatgagccg aagtcggaag aagtagcgat
    12241 ggagagcaac ggtaaggcgc tggccgatat taccgatccg gcaaccggtg cggttatcgt
    12301 caagaaaggc caacaactta gctcgttcgc ccaattgcgc gatgacggta caacctcctg
    12361 tggctgctgg attttcgccg gtagctggac gccggaaggc aaccagatgg cgcgtcgtga
    12421 taacgccgat ccgtctggcc tcggtaacac gctgggctgg gcatgggcat ggccgcttaa
    12481 ccgccgcatt ctgtataacc gcgcctccgc agatccgcag ggtaacccgt gggatccgaa
    12541 gcgtcagttg ctgaaatggg acggcactaa gtggaccggc tgggatattc cggactacag
    12601 cgcagcgcct ccgggtagcg gcgtcgggcc gtttatcatg cagcaggaag gcatggggcg
    12661 tctgtttgcc ctcgataaga tggcggaagg tccgttcccg gaacactacg agccgtttga
    12721 aacgccgctg ggaactaacc cgctgcatcc aaacgttatc tcgaacccgg ctgcgcgaat
    12781 ctttaaagac gacgccgaag cattgggtaa agccgataag ttcccgtatg tcggaaccac
    12841 ctatcgtctg accgagcatt tccactactg gaccaaacac gcgctgttga acgcgatttt
    12901 gcaaccagaa cagtttgtgg aaatcgggga gtcgctggcg aataaacttg gcattgccca
    12961 gggcgatacc gtgaaagtct cctccaaccg tggctatatc aaagccaaag cggtggtgac
    13021 caaacgtatt cgcacgctga aagcgaacgg caaagatatc gataccatcg gtattccgat
    13081 tcactggggt tatgaaggcg tagctaaaaa aggctttatt gccaatacgt taacgccatt
    13141 cgttggtgat gcaaacacgc agacgccgga gtttaagtcc ttccttgtga atgtggaaaa
    13201 ggtgtaacgg agacgacata tggcttatca atcgcaagat atcattcgtc gttccgcgac
    13261 taacggtctg acccccgcgc ctcaggcgcg ggacttccag gaagaagtgg cgaaactcat
    13321 cgacgtcacc acctgtatcg gctgtaaagc ctgtcaggtg gcgtgttcag agtggaacga
    13381 cattcgcgat accgtcggca ataacattgg ggtgtacgac aaccccaatg atttaagcgc
    13441 caaatcgtgg acggtgatgc gcttctcgga agtggagcag aacgacaaac tggaatggct
    13501 gatccgcaaa gacggctgta tgcactgttc cgatccaggc tgtctgaaag cgtgcccggc
    13561 ggaaggtgca attattcagt atgccaacgg tatcgtcgac ttccagtccg agcagtgcat
    13621 tggctgcggt tattgcattg cgggctgtcc gttcgacatt ccgcgcctca acccggaaga
    13681 caaccgcgtc tacaaatgta cgttgtgcgt tgaccgcgtg gtggttgggc aagaaccagc
    13741 ctgcgtgaag acctgcccaa cgggcgcgat tcacttcggt acgaaagagt cgatgaaaac
    13801 gctggcgagc gagcgcgtgg cggagctgaa aacccgcggt tacgacaatg cgggcctgta
    13861 cgatccggca ggcgtcggtg gtacacacgt tatgtacgtg ctgcaccatg ctgacaagcc
    13921 aaatctgtat catggcttgc cggagaaccc ggaaatcagc gaaaccgtga aattctggaa
    13981 aggcatctgg aaaccgctcg cagctgttgg ctttgcggct accttcgcag ccagtatctt
    14041 ccactacgtg ggtgtcggtc cgaaccgtgc ggatgaggaa gagaataatc tgcacgaaga
    14101 gaaagacgag gagcgcaaat gaaacgacgt gacaccatcg tgcgctacac cgcgccggaa
    14161 cgtatcaacc actggatcac cgccttctgc ttcatcctgg cggcggtgag cgggctgggc
    14221 tttttgttcc cgtccttcaa ctggttgatg caaatcatgg gcacaccgca gctggcgcga
    14281 attctgcacc cgtttgtcgg cgtggttatg tttgcctcgt tcatcatcat gtttttccgt
    14341 tactggcatc acaacctaat caatcgggat gatatctttt gggcgaagaa tattcgtaag
    14401 atcgtcgtca acgaggaagt aggtgacacc gggcgttata acttcggtca gaaatgcgtt
    14461 ttctgggcgg cgattatttt cctggttctg ctgctggtga gtggcgtgat tatctggcgt
    14521 ccttattttg cgcctgcttt ctcaatcccg gtgatccgat tcgcgttaat gctgcattca
    14581 tttgccgcag tggcgttaat tgtggttatc atggtgcata tctacgccgc cctttgggtg
    14641 aaaggcacga ttaccgccat ggtggaagga tgggttacca gcgcctgggc gaagaaacat
    14701 cacccgcgct ggtaccgtga ggtccgcaag acaacggaaa agaaagctga atgagtattc
    14761 gcataatccc gcaagatgag ctgggttcga gcgagaaacg tacggcggat atgattccgc
    14821 cgttattgtt ccctcggctc aagaatttat acaaccgccg cgccgagcgt ctgcgcgagc
    14881 tggcagaaaa taatccgctg ggtgattacc tgcgctttgc tgcgcttatc gcccacgccc
    14941 aggaagtggt gctgtacgac catccgctgg agatggatct gactgcacgc attaaagaag
    15001 ccagcgcaca aggcaagccc ccgctggata ttcacgttct gccgcgtgat aagcactggc
    15061 aaaagctgct gatggcgcta attgctgagc tgaaacctga aatgagcggc ccggcgctgg
    15121 cagtgattga gaatctggag aaggcatcga ctcaggagct ggaagatatg gccagcgcac
    15181 tgtttgcctc tgatttctca tccgtcagta gcgataaagc gccgtttatc tgggctgcac
    15241 tgtcgctcta ctgggcgcag atggccaatc tgatccccgg caaagcccgc gctgaatacg
    15301 gcgaacaacg tcaatattgc ccggtatgtg gctctatgcc ggtgtccagc atggtgcaaa
    15361 ttggcaccac tcagggtctg cgttacctgc actgcaacct gtgtgaaact gaatggcacg
    15421 tagtgcgcgt aaaatgcagc aactgtgagc agagcggcaa actgcattac tggtcgctgg
    15481 atgacgaaca ggccgcgatt aaagccgaaa gctgcgatga ctgcggcact tacctgaaaa
    15541 ttctctatca ggaaaaagaa ccgaaagttg aagccgtggc agatgacctc gcctctctgg
    15601 tactggacgc acgaatggag caagaaggct atgcccgcag ttccatcaac ccgttcctgt
    15661 ttccgggtga aggggagtaa tctctgacgt tttccgggtg gcgctggtcg cccggattac
    15721 agctcatagg gtgtgacgac accatctctc gtattccgcg ttaccacctc aagctatgcc
    15781 gccagtgcct gttttgcctc aatttaccca cccccacatc aataaggtta tgggtatgag
    15841 actgatgata agcgtaagat tgcctgatgc gccgcgctta ttaggcctac caataaactg
    15901 caatacattg aatttatacg actttgtagg ccgggtaagg cgttcacgcc gcatccggca
    15961 tgaacaacgc acacgttgtc agcaatctga ttgatatcat tttttcccat taccattcct
    16021 cgcgcagctt acgctggtat tcgacaccat cttcctcacg cccttgccat atgccaaaag
    16081 cactggttct tactgtttgc gattgattta ccaagtattg atctaccgct tcccgtaata
    16141 gctccgtacg aggaagatta cgctgcacct caagatcatc aagttgctta atcacctcat
    16201 tcgataaatc gagtataatt ctgcccatat caatacctgc cagagagttc ataaatattt
    16261 gaccaatatc atttgatatc tatggagaga aaagtaccct tgtcgaatct ttaaagaaag
    16321 ctcatttacg catcattttt tatttttgcg aggcgttttc cagatatgat gtaacagttg
    16381 cagttattat aagttcaagt aaaatagcct tgtattaata ccagaataag ggcatctgtt
    16441 ttttatattc aagaatgaaa aatttttgtc attccttatg ctccttacac tgatcatcaa
    16501 caccaactac ctcccccttt tgcctgcctc cctttgcttt acgcaccggg aattgctgac
    16561 gttccagata ttgctctacc gcttcgcgta aaattgcaga tcgtgaaacc ttacgccagc
    16621 cccgaagctt gttcagccgt ttaattgcct cttctgataa atgaagaaaa accgtattca
    16681 tcgccattgc caccctctta catatattac tcattaatgt atgtgcgaaa attacgccgg
    16741 aaaattaatt aacgaaagcg gatttttgca tgttgttttg tttttgcgag acgttttcca
    16801 gaaataataa gagcgttgca gttgtttcat ttgttactca aaaatggttt agttatcgcc
    16861 caggatgagt tatgggtgct atgaatatat tatattcagg gatatttatt ttttcattgg
    16921 atctttattt gcataatctg aatggtgtac ccgaaaaatg tcagcatcct aacagcacca
    16981 ggatttaagg tgaaattaat cttccatcac agaccggtca gtactgccac caaccggtct
    17041 ttacttgctc atcgttataa ttttgttggt aacagttcgg gaaacaagga attccttccg
    17101 gaacaccccc ggcatcatcc gatagcattg acgtcaatca tcggctcgac ggtgagtttt
    17161 tttaatcgca tcatagggtg gcaacgctca gcacgatacg ggtcataaca aaactcctga
    17221 gcagcggcac tatgccgctt tcacaggagt tctgaatgct ggtgctttct gtatggcttc
    17281 gccatgcctc tcgcggctaa gcgcctgcac acatctctct ttgtatttca tttttgcaat
    17341 gcattggccg aattctgata atcctaatga gttttactct tcagactccc atccctacgg
    17401 tatacgagtt agattcatga atgaaatcga catttttaaa aaacgaatct tgtaaaacat
    17461 ccgtgttgat attaccttgc aacccaacat taaatttgtt agagagatga agaactcgaa
    17521 tatcaaaaag gtcttttaac atcattactt cttcggctgt taatgaatcg caactaaaat
    17581 atccctgttg atcaaaaagg ttttctattt ctccatcatc ggcgaggcgt ttttcaaaga
    17641 gtgtttgcaa catcgagttg ccaaggacaa tggatatcgt gcgatctttc gcagatttac
    17701 accccatcac aacggcaaca ttcgtatccc cctcaggagt ttcatttatc gctgtcgcta
    17761 aaatggtggt caaacacgca aacggaactg tatttctttc gtgatttaat ttacctacca
    17821 gttcattcca tttatcaaaa gccgggcgat atttgttgcg cacagaagta tccgactggt
    17881 taaacaattt agccatgcga ttttttatca tctcgatatc cggcgaacca gaaagacttt
    17941 gcgcataata cggtcctgat gatgttgcat acattaaagc cgcgcccaca cctgtcgcca
    18001 tgatgggcgc agccacggtt ttggcaaggg cgacgctgcg atggctatgc gcttcacagg
    18061 ttaaaggaac atattccact gactgcggta attccttaca tgcctccctc atcattgccg
    18121 tatgctttac aaacaacggt ttttcactgc ccaaaaaaac tttctgctta attgcactaa
    18181 gaaaacgtaa atcaaaaatg attgtttttg gtttattatt ttttggcaca tttcctatta
    18241 tcccgctgag aacctttctt gctgcatgga gatttttttg cacagcacat aacacaattg
    18301 acgaagcctt ctctactgcg tcattgattt taattttaaa atccgcatga tatacgttga
    18361 cgggcaatac aagtatgtct ggcgctacat ctttggctga gtatttattg gtgatcgacg
    18421 gtcgtgaatc cacgagaaaa cttagctgca aattatgctc tatattacca gctgtttttg
    18481 tggtgtgatt aaacatagag atgccacctc tcattctttc acacatatcc tcaacttttt
    18541 cacttcgttc ttgtggcaat ttggctttaa cgttaatttg atgattttta cccgtgcatt
    18601 ccttaactac aaggggaatg ttatctaaag tgccatacag gctaataacg tcaccaggat
    18661 ttttatcatg taagtaagtt tgattaatac tcgaagcatt cacaaagagg tcaatattat
    18721 aactgtttat aacttcacct acatcaaatg tgtagtgctt actctggagg ctgccatcct
    18781 tatttaattg tatatcaatc aatatatccc cggactttgt caccatatct ttgccataaa
    18841 ccatttgcat agccatttcg aggagatttt tatctaactg attgcgggcg ttgagtaagc
    18901 gactaccaaa taatttatat actgttcccg tcattgccag ggctgcgctt tctggtactt
    18961 tgcgtttcca cagtgaggtt tctctatttt taaacaggtc atattcatta ttactcgaca
    19021 aaccatattt cagttcgtta acacaatgga acagttgttc ctcaccttcc attttaaggc
    19081 gatgtgaaaa atcatttaat agcgtttgct tcggtgattc attactcatt agttgttttt
    19141 ctaaattaac aacagaataa ctttcttgtt gaatgagatt aatattggca accattacag
    19201 cctcctttcc acagttaaga gcaaacatac aacataatta tttataacat ccagagcagt
    19261 aaatgaatga tactgacaat gatgccagcc cattatcttt gcataatatt tgttagctat
    19321 agattttaaa aatatttatg agaaaagtat ggcaccagag ggggttttga ctatcaaaaa
    19381 tcgctcaaac atgaactaag atgatgaatt aagcattatc tgcgataatt gccatacagc
    19441 tctacaaata atatttaaat gctcctgaac agtgatgtag caagacacgt atggagccaa
    19501 cgaagaataa aaatttatag agttcttatt caatataacc gaaatagcca aggactaata
    19561 taaatgacct gaatgagaga ggcagcaccg cccctctcca tcagctactc ttcttcgttc
    19621 ccgccctctt catacgggcc aaatggcttc gcgggcagaa cgatatacgt gccttcaaac
    19681 actgcacccg gcgtctcgtc gccaaagatt tcgacctgca tctgcacccg tgcttttcgt
    19741 ccgcgcgcca gacggtcgag atcgccgctt aaggcaccga ggtcggctac cgcatgaggt
    19801 ttaccgctaa tcggcttgct gtagcggata tgcgcatccg caagaataat cgttccgccg
    19861 aggtggcgtt cacgcagcat cagccagata agtccccaac cggtgagcgt cgccagtgag
    19921 aataaactcc cggcaaacag cgtatggtgc ggattctgat tgccggtttc tggcatggta
    19981 gtgataaatt tttgcccggt atattgctga atgcgcacgc ccattttttc actaagcggg
    20041 atatgttcgt accacgcctg ttgcagctgc gcgcaccagt cgccgcgatg cagaatatca
    20101 tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa tcggcgtggt ggttggcgtg
    20161 gtgatttctc cctgattaac aaaccccagc ttggcgaaaa actccaccgc gtcttcacgg
    20221 gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca ccgactccag ggtcatcgcc
    20281 atcagcgtgc ctaacccttt gtcctgcacg tcgggatgaa cggccataaa gcgaatggac
    20341 gcttcattgt cggcattaat atacagtcgg cctaccgcca ccagattacc ctgctcgtcg
    20401 acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc gttccgaacc ttttggttga
    20461 tgcaggggct tacgcaacat ttcccagcga aactgatagt aacgctctaa ttcttcttct
    20521 gtttgtggaa cccgaaggtg atacatagcg atactctctc ttgtttcccg tgacaaccct
    20581 ggaagctggc tcatacctgc aaccagaatg tcacggggcc atcattgacc agcgatacct
    20641 gcatatccgc agcgaagcgt cctgtttgcg tgttcatctc ttgctgacgg cagcgttcga
    20701 cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc acctttggag aaacttggac
    20761 gcatcccccg ttcggtatct gcggcgaggg taaactggga aaccaccagc acactgccgc
    20821 ccgcctgttg cacgttgagg ttcatcttgc cttcggcatc gctaaagatg cgatagccga
    20881 gcacacgctc gcacagacgg tttgctttct gttcgtcgtc atccttttcg acacccaata
    20941 acaccaaaag tcccgcgcca atttcgcccg tcacttctcc ctccacggtg acgctggcac
    21001 gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct tcttcttgtt cagctgcttg
    21061 ttttagtttg cggtattccc cgagagtgac agtaatttcc gcgccaagca agacgataca
    21121 ccatgtccag tagacccaaa caaagagaat ggggatcacc gccagcacac cgtaaatgag
    21181 ctgatatgac gggaacatgg tgatataaag cgcgaaaccc ttctttcctg cttcgaacag
    21241 gagcgcggcg acaaacgcgc cgacaatcgc gtcgcggtta ggtacgcgga tggtaggaac
    21301 aatgctgtac agcaaccaga aggagatcca cgacaacagc agcggaaaaa tacgcagcac
    21361 attgtcgatg acagtattga gatcgctcgc ccagcgcagg gagagcaaat aggaactgat
    21421 cgccagactg gcccctgcca gcagcggccc cagcgttaaa atcatccagt acacggcgaa
    21481 cgagtaaatt tttggtcgag ctcgtttact gcgccagatg gtattcaacg cgctatcgat
    21541 ggagtacatc aacaataacg cggtgacgat cagcccgcac gccccaacgg cggtcatctt
    21601 gttggaattg gcaacaaatt gttcgatata ccgctgaata acatcgccag tagcaggcag
    21661 aaagttggca aaaataaagt gacgtaactg aatgctgacg tcggaaaaca tgggaaaagc
    21721 ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat gagagcaacg acacataggc
    21781 aaggttacct gccagggttg tcatattgtc ctcatcaatg cgttgccaga gtagttttag
    21841 ccaggcccat agtggacggg tacggtgcct ggctttgtcc tgaatggttt ttagcataac
    21901 accttcgcga aatagtccgg gatggtggtt ttatctttca ccagaatact ggtaatgcct
    21961 agctgattag ctccttctat attatcggcg ttatcgtcga aaaaggccgt atcgctgggt
    22021 gaaaaacctt ccgcctgcaa aacatgctgg taaattcgag cttcaggttt gcgcatcccc
    22081 agatcttgcg acagatagat atggtcagca gcatcacgaa tttccgggta ttcttccggc
    22141 cagaaggtgg tatgcaggcg gttggtattg gaaagcacca ccacgcgatg cccctgctca
    22201 cgcagtttat gcatgatggc gatcacttcc gggcgcagcg caacaaacac cgcctgccag
    22261 ccgtgagaga actgctcgta gcttagcggt agagccatct catgacacag cgcctctgcg
    22321 aacgcttcgt cgctaatttc cccacgctca tgctgatgaa acgcctcccc catatgaaaa
    22381 ctcttcttaa gcgtagccag cggaatacgc gttaaatcgc tccaggctcc cagcacacgg
    22441 ttaaagtcga tatcgacaat cacattacct aaatcaaaga tatagagcat ttttgcctcc
    22501 tttcgcgcca caagaaatta actgtagcgg gaaaggatgg gtttggctat gtggcaataa
    22561 gggtaatctt gtgatgctga ttgcgaaaaa gcagagtaat ttcactggct ttttcgcaat
    22621 agatgaatgg ttaagattca ggtgtaaatt gccaacgagt ttcgataacc agcggcctat
    22681 ctggtgtcac caattccggc gttagattta ttgcgttagg tgcctgagtc agcgggttaa
    22741 tacaggtggc atcgggctgt ttatcaaata ccaccagact gtttgcagaa gagatcatgg
    22801 tcatcgcgag tttaccaggc cacagtaact taaccttcac cccgtcataa aaaccaaaac
    22861 agtcatccca gggaccgact tgaggagaga tgcgatttcc agttggcagt tcatcactac
    22921 ccgcttcttc ttgccagtcc gcatcaaaaa gcacctgcaa cgattctgta ttttgcggag
    22981 tcagcttttt cgcaaaccac ggatgccagc ctgcggaggc cgggaatgta tcggcatatg
    23041 aatggacttc cagttgcaaa accagcgcat cgttctccag caaaaaagtc tgaatcactt
    23101 ccccttgcca aggccacggt gaagccaggg gcatacgtaa agtcagggaa tctgctgttt
    23161 tgtcgatgat ttcccacgtt gagtaacaag ccataccatg caacgcatgt ggtggtttat
    23221 tggcgggtaa agaataacat tgcccaccag cattcagcgt cgcattcccc agtcgccctg
    23281 cccacggcac cattgggaaa cagccatact gaaaagctct acgctgcggc tgccattggc
    23341 gtaatacttc agaaccaaat gcctttaacg aggttatcct ggcaccatca tgcggataaa
    23401 tctgcaggtg aatattgcct ttttccagcg tcactaactc tcctacaggc ccaggccatg
    23461 gcgcgtcaaa cgcctgttgt agcgccaggt aatgctcgtt ttttcgttca gttttcatga
    23521 gattgttccc tgatgtagcg gtaagaagcg gtttcttgtt catcggtcgg ctcaatcagc
    23581 cagacgcaaa caaaggaaag aactgagaca gcaaataaaa acagagcgac ataccacggt
    23641 tttcctcccc ctagcgccag caaacttgag gcgataaggg ggcttagtcc gccaagaatc
    23701 gcggaaaatt gataagcaaa agaaaggccg gtatatcgta ctttggtacc gaacatgcgg
    23761 gtgaaaagtg ttggctgcac ggcaaacatc atcgtcggac ctaagttgta gccgaggatc
    23821 atactgcacc agatgatgag cgtacttttg ctatccagta ataagaagaa aggaaacgcg
    23881 aacaggacac aaaatcctgc tcccgacaaa tacagacttt tttgacctat acgatctgat
    23941 aaccatccaa ccaatggaca actgaaaata ccgatcgccg aggcaatcaa catacccgtc
    24001 aggggaatat ctcgcgataa tgctaattgg ctggaaatat agacaatacc aaaggcgtta
    24061 ataatattag aggagacact ttccgccagc ctcgcaccca gtgccaataa aatatttcgt
    24121 ggatgttttt tgaataattc aatcaacgga ggaacggact tttctttatt attcgtcgtt
    24181 ttttgctttt gaaaatccag cgtttcttcg gtatgcaggc ggataaacag cccaacgatc
    24241 aacatcaccg cagaaagcca aaacggaatg cgccatcccc aggagagaaa ctgctccgaa
    24301 gtaagaaaat ggttacagag agcaaaaata ccggttgcca gcataatacc cacagacgca
    24361 gccgtttgcg ggagtgaacc taagaatccg cgtcgggatt cgggcgctga ttctatggtc
    24421 atcaatgccg ctccaccgta ctcgccacca agaccaaagc cctgaatcaa acgcagcacc
    24481 attaatacaa gcggtgccca aatgccaatt tcctgatacg tcggaataaa accaatcaaa
    24541 aatgtggagc aaccgacaat agccagcgtc cagataagtg ttatttttcg cccgatttta
    24601 tcgccaaaat gaccaaagac gatgccccct aaaggacgag tcagaaaacc gacaccaaag
    24661 gtcgcaaagg aagctataag acctataaaa ggatcgctgt ctggataaaa cagcggtgca
    24721 aataccagtc ctgctgccgt gccaaaaata aagaagtcat accattccat taacgcgcct
    24781 gcgaaactgc ccataacgac acgtctcatg gctttaccgg aaagcccttc acgcggtgcg
    24841 ggtaactttt cagacataat tttcctctcc tggtaggcga gaaatctggg atacgaacca
    24901 ctaaacgtgg gcagtttgaa ttaatgcgca tcggcttaag ccgatgcgca ttaaattatt
    24961 tcttcaattc agccagaacc tctgcgtcct cagcatcaac atttgcaccc agtggttccc
    25021 aatgaatcaa tacgcaggtc aggaacccca ggaatggcac gattgccaac cagtaaaacg
    25081 cttcagtttt caacgcaccc cacagcatcg gccagaaaat gagtcctgca attgcccctg
    25141 tgcgcataat agcgcgggca aatccgaccc cagtagggcg aatagcggga ggataagaga
    25201 gtgtggcaat ggtcatgccc agcccaccag gacccgctga atggaagaaa ataattgatc
    25261 ccaataaccc taaagacagc cagggattag tcgttgccag tgcgcctaac gacagcaatg
    25321 ctaaggtcac aacagcaaat ccatacatcg actgtcggcg agtacccagt cgttggagaa
    25381 taaacgagcc gatcatcccg cctgtcaccc cacaaagggt attcacgacg gcagaaccgg
    25441 tcaacgcgcc agtaagcccg ccacttatta ttcccgccaa tgtaagagga agataaacac
    25501 ctacggcgtt atattgccac gcctgcatgg ttgccacgac acagccaaga atggtgcgtt
    25561 tgcggtaacg atcattaaat aagatgccat atccaccact gtatttattc tctgcttttt
    25621 ccgagaattt cgcttctgtt gtactcgatg ccgcaacgcg agcctgaaca ccataacgtt
    25681 ttcccagaat gtcgcacgct tcctggtaac gcccgacgcg tgcggcccac attgcggatt
    25741 caccaataaa gaagtaacgc aaaatcatga agatgactgc aaaaatagct ccgatgaata
    25801 ttccgtaacg ccacaattgt tcttcagcga tacccgacag aagcagcggc aaaagtacca
    25861 aataaacaac ggttgtcgaa acataccagg ccatttgcca gaggttgacc gacgttccgg
    25921 ttttcttcga cgttgaacca cgaagctctg ccacggcatt ggttgccaaa gggaagtcga
    25981 tgcctaaacc aaagcccatg atgacgcggc acaccagcac ccaccagata ctgggagcaa
    26041 tagctaccgc tgcagcaccg atggtgcaca gccccatgcc aataataaat gcccgctttt
    26101 gcccaaagcg gtttattact cggttcgcta ataaaccgcc aattaacgcg gcgattaata
    26161 ctgatgcatt gacggttgca gcaagacccg gcgaaatgcc gaattgagcg gcaatgtatt
    26221 tattaccaaa ccccaccatc gccgcctggt aggcatcaat caatatgccg cccaaagcaa
    26281 taaaaataac gccgatactg gtgttgattg cagagttctt attaacaata tcaattacgt
    26341 cctgtggccg actaattgaa taagttgcag gactggtgat ttgactcatc gcagtgtcct
    26401 ttttaattgt tgtagggtgg cgctgacgtg acgcagcgcc agacagagat ttagaaaatc
    26461 attccggcgc aaattgcagc atgaccttta tggagtcatt ttgctgcgcg cgttcgaatg
    26521 ccgccgctgc atcctcaatg gcaaataaat cggtaaccag ttcactggta tcaaaccgcc
    26581 catccgccag ccattcgata acagcatcga aatcttcagg gatgtacatg ccagagttga
    26641 gcaagtcacg ctcgaaacgt tgcatcatcg gtaatggtat ttcacgcggc ccacttggta
    26701 cgcccatgca cacgacggtg cctccggcat atacacgggt acatgcatcg ttaagcgtgg
    26761 cctgcgctgc aacgacatca atcgcccccg taaatcgcac atctgcggcc agctcacctg
    26821 gtgcccaaac ttctgctgcg cccagcttta acgccagcgc gcgtttggca gcgtcaggtt
    26881 caatcacggt aatcttacct gcccccataa tgcgcagtgc ctgcacgatg gataagccta
    26941 tcgttccggc accaatcacc agtacgcttt ccagagaagc tttcggcatt cggttaacgc
    27001 agtgacgggc acaagccgcc ggttcggcaa aggccaacac ttttagcggt aaagaggctg
    27061 gtgcgacatg gcaattacgc gcaggaacaa tgtgttgtga gcgagcaaag cccggtgcgc
    27121 gaaaaccagc aacctgcggt ggttcacaaa gattaaaacg tcctgctttg caggctcggc
    27181 attccatgca agccatgatg ggatcgacca caacatgatc gcccggctgt acatttttaa
    27241 cgtcgctgcc aacttccgta acgcgcgctg caatttcatg accggggacg acaggtggtt
    27301 tggcaaacgg atgaccgcct ttcagaacat gcagatccga accgcatatc ccatagaaca
    27361 caggcgcaat ccgcacttcg tgcggtttaa gttcctggtg tggtacttca acatattgag
    27421 tggtgacttt cccaatatct gaaaataaaa cctgggtaat tttatccatt gttattctcc
    27481 agaatcgata tccatagggg attagcgttg cgcgatacct tcatcgacaa tttctgccaa
    27541 ccgttgtaaa cgaggaacag agacatcgcg cagcataatt tgtggatctt gtgcaccaac
    27601 aaccgacgcc caaaccgctc gtccagccag gaagcctgac gcaccacctt tcatggcaat
    27661 acttactgca cggccaaaaa tatcggcatc aacgccagaa gaaagaatta cccacggcat
    27721 cttcatctga tcgttaagtt gctgacaggc ggcaagtaag gttttttcat cgcctttgcc
    27781 ccctaatggc atctcggctt tgtaaagatc agcctctgtt ccaccaagct ctgcggcggc
    27841 ggcaacaatg gcgctttcac gatcaaagtc ccaaccgcga cgaggtggac gcaccacagg
    27901 ttcaataatg ctaaccagcc cggcactacg gcagcgacga acaaatttat caaccattgc
    27961 cagacgttcc tctgcgggtt catcttcacg ccagagtaca agtaatttca ttgccttagc
    28021 ccccatttca cgggctttat gcgggtcgac ggacatatct atttcaacgc tatcaaccgg
    28081 gatgccatta cctggaataa aaagatcggc agcgacaatc aggccgcaag aattagcaac
    28141 agcgccctgc tcgactattt gctcaagaca aaattgttta tccgccagaa cggcagaagc
    28201 ataaggagaa aggattcggg tcgcggcaac tttaaaatcg gtgagtacac tgtcggcgat
    28261 gggttttggc tgccctgctg ccgcaaacat caagcgcatg gcctcacgct gatcgacagc
    28321 taacatggca aatccaccac ctgggcggga gatatctttc agcgtaaatg gggtgaagtg
    28381 ggatgacata gaaactcctg ttaatagata aaattaaaga ccagcctgct ggcgaacctg
    28441 atttagcagg gcactccagt cctgccgccc tttgccgtct tgacttgctg cagcgtaata
    28501 actttctgct gcaatcccca tattgagcgg tgcccccgtc tcccgagcga cgtcaactgc
    28561 aatacgcaga tctttcaacg ccagatccac catgaaggcg ggtgaaagat cgcctttcag
    28621 cactttgcca ggccaggtgg tcgtaaaatg tcctttccct gctgccgtac cactcatcac
    28681 cttaattgcg acatcaagat ttaatcccag tgactcacat aacacagcgg cctcggagga
    28741 cagcgcatta agcgcaatgc tcatatagtt gttaattagc ttcacgcgga tccccattcc
    28801 agggccgcca gcctcgacca gctcattacc catgcacatg agaatcgggc gtgcacgtgc
    28861 aatctgctca tcagtgccac ccgctaaaat cagcaatgtg ccatcaatcg cattcacgga
    28921 cgttctgccc acgggggcgt ccatcatact gatgcctttt tcttgtaact cccgtattaa
    28981 tgcatcggtc tgtaagggat gaatagtcga catatcaatc aataacgcgt cagatgaaac
    29041 agcctcacag acgccttttt cccccaacag aacctgtcga acaatgtcac cattgggaag
    29101 catagtgatg ataaattcag cgcctgctgc tgcttccgct ggggtttgtg ccgcagtggc
    29161 cccctgcgta actagcgcat cgacggccag tgcattgaca tcaaatacct gcaatgaatg
    29221 ccctttatgc aatagattgt tagccattgg gctacccatc tgccccagcc cgagaaatgc
    29281 aatggttgcc atgaatactc tcctgtctga cattgacaaa ataatgacaa ttgaaaattc
    29341 gttttcgtct gtttttgagc atatttgtaa tttatgttca aaaacacgac atacatcact
    29401 aatttgatca tttgtgtcat ttaaattgac caaagacgac aaaattaaac aggacaagac
    29461 aaatatgacc agaattgcat gcgtgggtat cacagttcag gacaggattt attctttgcc
    29521 cactctgccg gagggcggcg ggaaatatca ggcaaatcat tatcttgaga ttggtggcgg
    29581 cccggcagca acagccgctg ttgcaatcgc aaggcttggc gtggaagttg atttcatcgg
    29641 tcgcgtcggc gatgacagtt gtggaaatac cttacttgca gaacttgagg gttggggcgt
    29701 caatacagca ttttgccgcc gatatcctaa cgcccgttcg tcgcaatcag cgatcctcgt
    29761 tgatcagcat ggcgaacgga ttatcgttaa ttatccaagt cctgatctcg gtactgatgc
    29821 cgaatggtta gaagcgatcg atttctctcg ttatgatctg attctggccg atgttcgctg
    29881 gcatagcggt actgaaaaag cattctcgct agcgcgcctt gcgggtgtga caacgctttt
    29941 agacgccgat atgacaccgc aggatatttc tccacttgtc gcgcttgccg atcacgccgt
    30001 gttctcaacg ccagggctga aacgcatgac tggcctgcag tcacctgaag aaggtttgtt
    30061 ccaggcaacg acacaaacag caggtaaagt gtatgttacg ctgggttctg aaggatcgtt
    30121 gtggattgaa gatggtcatt tgtgtcaaca ggaggcattt tctgtcaatg tggttgatac
    30181 cactggagca ggcgatgttt ttcacggcgc actcgcggta gcgttggcag aaaaaatgcc
    30241 aacaaaagag gctattcgtt ttgccagtgc ggttgccgcg atgaaatgta cccagcctgg
    30301 cggtcgggcc ggcattccta atcgtgagca aaccgaatca tttttgtcac tttatgcgta
    30361 aaatggtaaa catctaccga ggaggggcaa gggagtattc atgagtatca tcgaagtgac
    30421 aggtaatcca cgccatgacc aactcgttca tctaatcgca gaacgcggtt atatgaatat
    30481 tgaagaactg gcacaactgc tggatgtttc aacgcaaacc gtgcgccggg atatccgaaa
    30541 actcagtgag cagggcttga tcacccgtca tcatggaggg gccgggcgcg tctccagcgt
    30601 catgaatacc gcttttgagc aacgggaact ttcgctcacc gccgagaaac gggcgatcgc
    30661 tgaggcagtc gccgattacc ttcccgaacg ctgtaccgtc tttatcacca tcggtacaac
    30721 cgtagaagcc gttgccaggg cattactcaa ccggcgtgat ttacgcatta tcactaacag
    30781 cctacgtgtg gcacagattc tttataagaa tcaggatatt gaagtgatgg tgccgggagg
    30841 cactttacgc gctcataacg gcgggattat cggcccagga gccgtggatt ttattgaagg
    30901 cttccgcgca gattatttaa tcaccagtat aggtgccatt gaacatgacg gcaccctact
    30961 ggaatttgat gtaaatgaag cgttagtcgc gagaacgatg attaaacatg cgcggaatac
    31021 attgttagtg gccgatcata cgaagtttgc cgcgtctgct gccgtttcaa ttggcaatgc
    31081 acggaatgtc agggcttttt ttactgatgc cccgcctccc aattctttct gccagttgtt
    31141 aagtgaagag aatgttgaac tggtggttgc cgagcaggaa gtttcctgaa attgaaactt
    31201 cgaaaaatcc gctggggatt cagcggattt tattttgccg gatgcggcct acggttgata
    31261 cggttttcgt tggccggatt aagcacatcc ggccattaat taagcttcag ccgccagcat
    31321 ctctttctta cgttggctaa tacgcttaac cagcattacg gaaaccaccg caccacagaa
    31381 tgcgaagcag gccataatgc caaacacgat tttgtagcca ataatccccg gatttaaatc
    31441 cagaatgtag ccatacagac tgaagcagaa catcgccggg gcgtaaccaa taaagctacc
    31501 caacgccatc gccgcgcctg ttttattttc agcaattttt gcttcgccga taggtgcaaa
    31561 aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg ccatccctaa
    31621 atagaccggc atactttcgt gaggtagcat aatcaacagt acgagcgcag cggtactgat
    31681 gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg aaatcatgcc
    31741 accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt aagcccccac
    31801 cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg tcaggccaca
    31861 gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatagttt tgtctttcag
    31921 caccgaggtc atactggtgt tttgcgatgc cccgtcttct tttttcaact taacggacgg
    31981 tgcctcttct ttgtcattca ggacaaagaa aataataata cctacggcaa tcacaataag
    32041 ggaatagaac cagatgcctg ctttaaaacc caataagcca ctgccaaacc aggtaaatac
    32101 tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt cgaagaagcc
    32161 aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga gcaagaccgg
    32221 ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga tgccccaata
    32281 ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg agaatgacat
    32341 ggtgtatctg cgcggtagtt tatcagcaaa ataaatagac agaaaaaagc ccactgtggt
    32401 aacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat ggaaatattc
    32461 ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg taccgccaca
    32521 cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat cccctaagcg
    32581 atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatatg gaatggtttt
    32641 attaactgcg tatgagttaa aacttattag ttgttcagaa gatggtttgc ctcttgtttg
    32701 atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc tttttcttta
    32761 cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat ctggcgataa
    32821 gggatccacg gattatcatc ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc
    32881 tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cacccaggca gataaggtgc
    32941 gttaataacg ctttgaacgg catatcaccc tgacctgaaa tacaggcttt atgacccagg
    33001 ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat atgcggtgcc
    33061 atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttcgc aaaatcaaac
    33121 agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccaactc atgacttttt
    33181 aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt cctgatacgt ttctcgtaca
    33241 taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc ataacgcgga
    33301 taaaaacgta cggacgatgc cccagttttc aacgcgatgg cgacggcttc gtcaatagat
    33361 tttttatctg aggcgctggt ttcaatatga atatcaaggt tcagacggcg ggctttatcc
    33421 ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc gccatccaga
    33481 acatggattt tgacgccacg tagattattt tcacttgcga tatcaagtaa atcagcaggc
    33541 aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata aagaggaaga
    33601 gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcatcataaa acacctacag
    33661 attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct ggacaatatt
    33721 gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg cagaaccgcc
    33781 aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt tttttactac
    33841 cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt caagttgttc
    33901 aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa ctttatgtcc
    33961 ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggtgtca ctgcaatcag
    34021 tggcgcgagc gtctcgggaa tatcgctggt acggcttttc tcggtaaacc cccaggaagg
    34081 ttcccgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg gactgtaatt
    34141 aaatcgcggc tgggtcacga accacccctt acgatcctta cggaagattt tggattctgc
    34201 ctcgaggttc aacaacgcct ggcgcagcgt catacgttta atgcccagca gttctcccag
    34261 ctctctttct gaagggagtt ttccacccgg cgtggtgatg ccatctttca accaccgatc
    34321 cagtttctct tttgcatttt ctacggtgga ttgattctcc gccattgttt aatccttttg
    34381 gtttaaacca gatgaagcat cttacgctta cgccaaaaaa gaaagcaatt aacggcatat
    34441 gactttgcag ataattgtga caaagctctc tattgacgta accacagact tataagggag
    34501 atactcgcat tggtgtgcgc tcacgacagg caaaaaaaaa cctgccatcg ctggcaggtt
    34561 ttttataact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc gacgacgatc
    34621 gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt ctaccagttc
    34681 gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg gaaccagaac
    34741 aacggcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt
    34801 tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt aaacttctgc
    34861 accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga acgcgaccgc
    34921 tttaccctga ccgttagaga tcagtacacc gttctgacgc tgacccactt cgcccggacg
    34981 tacgtcgtcg tagtggctga aggtggagta cagcagaccc gtaccggaag tcatggtcat
    35041 gaactcagaa cggaagccaa tcagaccacg gcttggaatc acgtagtcga gacgtacgcg
    35101 gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca gcgcctgcat
    35161 tacagaaccc tgatgctgtt cttcaacgtc cagagtcacg ttttcatacg gctcttgttt
    35221 acgaccgtcg atttcacgga agataacttt cggacgggat accgccagtt cgaaaccttc
    35281 acgacgcatg ttttcgatca gaacagacag gtgtagttcg ccacgaccag aaacgcggaa
    35341 cgcatcagcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt
    35401 cagacgatcc aggatctgac gagacgttac gaacttacct tctttaccgc agaacggcga
    35461 ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg ccggcagcgc
    35521 ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca ggcccgtgat
    35581 cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac cgaggtggcc
    35641 cagcacttta ccgactttcg cgttgcgggt tttgccttcg ctatcgatga tagtgacctg
    35701 ctggttcggc ttcactttac cgcgcttgat gcggccaatg ccgataacgc caacatagct
    35761 gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc
    35821 aggaacgtgg tcaacaatcg cctgatacag cggggtcatg tcttccgcca tatcttcgtg
    35881 gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc
    35941 gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc aatcaggacg
    36001 cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt aagcaaacgc
    36061 ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag
    36121 cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg
    36181 ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat
    36241 ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc
    36301 acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg ttttaccatg
    36361 gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt tgcctcaggc
    36421 attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg atcacaaaca
    36481 tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag cataaaaagg
    36541 gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag cactatattg
    36601 gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga taacgccttt
    36661 taggggcaat ttaaaagttg gcacagtttt cgctttatct tttttacggc gacacggcca
    36721 aaataattgc agatttcgtt accacgacga caatgaccaa tccaggagag ttaaagtatg
    36781 tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt tgatttgcgc
    36841 ttcaccgata ccaaaggtaa agaacagcac gtcactatcc ctgctcatca ggtgaatgct
    36901 gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg gaaaggcatt
    36961 aacgaatccg acatggtgct gatgccagac gcatccaccg cagtgattga cccgttcttc
    37021 gccgattcta ccctgattat ccgttgcgac atccttgaac ctggcaccct gcaaggctat
    37081 gaccgtgacc cgcgctccat cgcgaagcgc gctgaagatt acctgcgttc cactggcatt
    37141 gccgacaccg tactgttcgg accagaacca gaatttttcc tgttcgatga catccgtttc
    37201 ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc atggaactcc
    37261 tccacccaat atgaaggtgg taacaaaggt caccgtccag cagtgaaagg cggttacttc
    37321 ccggttccgc cggtagactc ggctcaggat attcgttctg aaatgtgtct ggtgatggaa
    37381 cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg tcagaacgaa
    37441 gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat ctacaaatat
    37501 gttgtgcaca acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc aaaaccgatg
    37561 ttcggtgata acggctccgg tatgcactgc cacatgtcgc tgtctaaaaa cggcgttaac
    37621 ctgttcgcgg gcgacaaata cgcaggtctg tctgagcagg cgctgtacta cattggcggc
    37681 gtaatcaaac acgctaaagc gattaatgcc ctggcaaacc cgaccaccaa ctcttataag
    37741 cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcacg caaccgttct
    37801 gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga agtacgtttc
    37861 ccggatccgg cggctaaccc gtacctgtgc tttgctgccc tgctgatggc cggtcttgat
    37921 ggtatcaaga acaagatcca tccgggcgaa gctatggaca aaaacctgta tgacctgccg
    37981 ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc actgaacgaa
    38041 ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcacggatga agcgatcgat
    38101 gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc gcacccggta
    38161 gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc gcaacaacgc
    38221 cgggatttag ttgccgtgga aactttcagc ccatctctgc atgggctttt ttctccgtca
    38281 attctctgat gcttcgcgct ttttatccgt aaaaagctat aatgcactaa aatggtgcaa
    38341 cctgttcagg agactgcttt atggcaacag gcacgcagcc cgatgctggg cagatcctca
    38401 actcgctgat taacagtatt ttgttaatcg atgacaacct ggcgatccat tacgccaacc
    38461 ccgctgcgca acaactgctc gcccaaagct cccgcaaatt gtttggtacg ccgttaccgg
    38521 aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagcctg gaggcggggc
    38581 aaggttttac cgataacgaa gtgacgctgg tgatcgacgg gcgctcgcat atcctttctg
    38641 tgacggccca gcgtatgccg gacggcatga tcctgctgga gatggcgccg atggataacc
    38701 agcgccgctt aagtcaggaa cagctacagc acgcccagca ggttgctgcc cgtgatttag
    38761 tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc
    38821 ttctcagcaa agcattacct gacccgtcac tgctggaata taccaaagtg attatcgaac
    38881 aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg gccgcaactg cccggtacgc
    38941 gcgttaccga aagtattcac aaagtggctg aacgcgtggt gacgctggtg tcgatggaac
    39001 tgccggacaa cgtgcggttg attcgtgatt acgaccccag cctgccagaa ctggcgcacg
    39061 acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta caggcgctgg
    39121 ggccggaggg tggagaaatc attctgcgta cccgcaccgc gtttcaactg accttacacg
    39181 gcgagcgcta ccggctggcg gcgcggattg atgtggaaga taacgggcca ggcattccgc
    39241 ctcatttgca ggatacgctg ttttacccga tggtcagcgg ccgcgaaggt ggcaccgggc
    39301 ttggcttatc catcgctcgt aatttgattg atcagcattc aggcaaaatt gaatttacca
    39361 gttggccagg gcataccgag ttctcggttt acctgcctat caggaaataa aggtgacgtt
    39421 tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt gggtgcttga
    39481 acgtgcgctc gctggagcgg gtttaacctg tacgacattt gagaacggcg cggaggtact
    39541 ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca gatatccgta tgccgggaat
    39601 ggacgggctg gcactgctca agcagattaa acagcgccat ccgatgcttc cggtcatcat
    39661 tatgaccgca cattccgatc tggatgctgc cgtcagcgcc tatcaacaag gggcgtttga
    39721 ttatctgccc aaaccgtttg atatcgacga agccgtggcg ctggttgagc gcgccatcag
    39781 tcattaccag gaacagcagc agccgcgtaa tgttcagctt aacggcccaa cgaccgatat
    39841 catcggcgaa gcgccagcca tgcaggacgt gttccgtatt atcggtcggc tttcgcgttc
    39901 ttctattagc gtgctgatta acggcgaatc cggcaccggt aaagaactgg tcgctcatgc
    39961 cctgcatcgc cacagtccgc gagccaaagc gccgtttatc gcgctgaata tggcagctat
    40021 cccaaaagat ttgatcgaat cagaactgtt tggtcacgag aaaggcgcgt ttactggcgc
    40081 gaataccatt cgtcaggggc gttttgaaca ggctgatggc ggtacattat tcctcgatga
    40141 aattggcgat atgccgctgg atgtgcagac gcgtttgctg cgcgtgctgg cagacggtca
    40201 gttttaccgc gttggcggct atgcgccggt gaaagtggat gtgcggatta tcgctgccac
    40261 tcaccagaat ctcgaacagc gggtgcagga aggtaagttc cgtgaggatc tgttccaccg
    40321 cctgaacgtt atccgcgttc atctgccgcc gctgcgtgag cgtcgggaag atattccccg
    40381 cctggcgcgc cattttttac aggttgccgc gcgagaactg ggcgtagaag cgaagttgct
    40441 gcatccggaa accgaagccg cgttgacgcg tctggcgtgg ccaggcaacg tgcgccagct
    40501 ggaaaacacc tgccgctggc taacggtaat ggctgccgga caggaagtgt tgattcagga
    40561 tttgcctggc gaactgtttg aatcaacggt tgcggagagt acttcgcaaa tgcaaccgga
    40621 cagttgggcg acgcttcttg cgcagtgggc agacagagcg ctacgttccg gtcatcaaaa
    40681 tctgctttcc gaagcgcagc cagagctgga gcggacgtta ctgacgaccg cgttgcgaca
    40741 tacgcagggg cataaacagg aagcggcgcg gctactcggc tggggccgca acactctgac
    40801 gcgtaagtta aaagagctgg ggatggagtg attcacggct tgtgtgtaaa gattgattat
    40861 tgagcgcaaa ttgctggtat tttgcgcttt actgttccga taagttcagt atgatcttgc
    40921 ccggaaaacg gggagagtca ttatgctgga atcaataatt aatctggtat cgagtggcgc
    40981 agttgacagc cacacaccgc aaactgccgt tgctgccgtg ctgtgtgccg cgatgattgg
    41041 gctgtttagt tgagtttcag attgctgatg aagtgctctt tgttcatgcc ggatgcggcg
    41101 tgaacgcctt atccggtcta caaaagcaat attttcaaca ggttttgtag gcctgataag
    41161 cgtagcgcat caggcaatct tgttctcagc acattgatgg cgctttcgtt ttctactttg
    41221 taaacgaagc gccattcact atttaaatca cccgagagaa ctgctgcatc cgcgctttct
    41281 ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga cctttcgccg
    41341 tcacctgtat tcccttctca tccacatcca ccagcccatc ttttgctaac ggggcgagca
    41401 gcttgagatc ttccgcaaag taatcagcga agtgcaaatc ccactgtttc tcaatagggg
    41461 cgtaatccag acggaagttg cagatgagcg acttaatcac atcgcggcga atacagtcat
    41521 cacgcgttag cgcaatacca cgccacagcg cattgccttg ttcatccact tgctgatagt
    41581 actgtttcaa ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa
    41641 cgcccatccc cagcagatcg gtatcgccct gagtggtgta gccctggaaa ttacgatgca
    41701 gcacgccttc acgctgggcc accgccagct cgtcatccgg acgggcaaag tgatccatac
    41761 cgataaactg atagcccgat tgcgtcagga aggcgatggt ttcctgcagg atatcgagtt
    41821 tttgctgcgg actcggcagg tcagcatctt tgattttgcg ctgagcagca aaaatggtcg
    41881 gcagatgcgc atagttaaag acgctcagac ggtcagggtt tagctccgcc acgcgtttca
    41941 gggtaaaggc gaaactttcc ggcgtctgtt tcggcaggcc gtaaatcagg tcgatgttgg
    42001 tggaggtaaa gccaatctca cgcgcatggt taagcagcgc aaagataaac tcttcatcct
    42061 gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc atactcaggc
    42121 gattaaagcc ctcggcgcgt aaatgatcga gtacatccag ttcgatttcc cgcggatcga
    42181 cttcgatcga aatctccgca tcggcattga actggaagtt ttcgcgcagc agcttcatca
    42241 ggcggctgat ttgcgcttta ttaagatacg tcggcgttcc gccgccccag tgcagctggc
    42301 tgacgtgacg cccggcgaac agcggtgcac gatggacgat ttcttgctcc agcgcgtcca
    42361 gatactgatc ggccttgtgc tgctggcgag taacaatctt attgcaaccg cagaagtaac
    42421 aaagcttatg gcagaacggg atatgtacgt agagagataa tggacgctca ggatagcgcg
    42481 ccacggcttg taaaaacgcc tgttcgccga agtcttctga aaactccagc gcggtcgggt
    42541 acgaggtgta tcgtggcccg gaatagttat atttctggat cagggccaga tcccagtcga
    42601 tttgctgtac agacatgctc actccttccg atggcgtctc tggcggctac ggcgtaccgg
    42661 cggatgcccg tttcgggtca tcagccggtt gcgcagccac ttttgtcgcc gcgacaaccg
    42721 ctgtagttta acgaataacc acaccagata acatataacc ggaagggtta tcagcagaac
    42781 gggaaggccc acggcggcaa tccgttagtt gccccgcagc agacgcatca tgtcttcttg
    42841 cttctcgtct tcttcctctt cttcgtcatc gtcataagag aggccgagtt tctgcatcaa
    42901 ctcatcaata cgatccagtt tagcatccac ccaggattgc tcttcggcac tcagggtttc
    42961 gcctgcttcc agacgttcca gcagcgcatc cagacgctca tccgtttcca gtaattccaa
    43021 ctccgcctgc ggtgaaagca taggtttctc actcttcggt ttgtgctgtt tggtgacttt
    43081 ttcagtcaca cccaatggaa taggggtttt actgccaata cgtggatctt tcggtgcgtt
    43141 ctggcctttg ctgcctgacg tgttgtttcc gcccgctgcg cggctgcccg gcgcatgacc
    43201 acgacgtttt ttctgacgct tgcggtcacg agcttcctga ttcagctctt cgcgcgtttt
    43261 gcgacgcgct tttgcatgac ctttgctgcg tgatggtgaa gatggtttca taatggttcg
    43321 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    43381 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    43441 tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac aatccgtgtt
    43501 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    43561 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    43621 atgggtgtta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    43681 attttcagag ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc
    43741 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    43801 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    43861 ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    43921 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    43981 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    44041 aaagcctggc tcgtacctca aaaaccccag gacgcaccca gcttatcaac ctgtttgaag
    44101 tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag
    44161 agatgaagcg caaatggcag cgcgcgctcg gcgagtacct cgaaaaacgt cagagcctac
    44221 aaggtctggt agtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga
    44281 ttgagtgggc ggtagacagc aatatcgccg tgctggtact gctgaccaaa gcggacaaac
    44341 tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg tgaagcggtg ctggcgttta
    44401 acggtgatgt gcaggttgaa acgttttctt cgctgaagaa acaaggcgtg gacaagctgc
    44461 ggcagaaact ggatacctgg tttaacgaga tgcagcctgt agaagaaacg caggacggcg
    44521 aataattttc ttaccttaat gcttatgccg gatgtggcgt gtccggccca taaattcaat
    44581 agattacaat tctaccgttg gcctgataaa catggtgcat caggcattgc ggattttttc
    44641 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    44701 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    44761 cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt
    44821 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    44881 gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    44941 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    45001 ttcgcgacgg catcgacatc atctctatga acttcaaata ccagttcatc gtgtacctgc
    45061 atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc
    45121 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    45181 gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    45241 agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atacttcagc
    45301 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    45361 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    45421 ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc
    45481 gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc
    45541 gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa
    45601 tcctctggcg caataaacgc ctggcggata cggcgacctt cttcgttacg taccggaatg
    45661 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    45721 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc
    45781 gatttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    45841 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    45901 ggtttaatgc cctgtttttc aaacagaatg gtttgtaact gcttggtgga agaaaggtta
    45961 aattcctcac ctgcaatttc atacgctttc ttttccagct cagccagacg aagggtgagc
    46021 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccattacg ttcaatgcgt
    46081 gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    46141 ttttgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc atcttcggcg
    46201 gcgtaacgtc cggcctcatc gagggcaatc tggttaaagg tcagttgatt tttgccttta
    46261 cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc
    46321 atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca
    46381 atcccacgca gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc
    46441 ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    46501 cgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata tgccgctacg
    46561 cctgactcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    46621 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    46681 gtttcttcat caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    46741 ggtgcttcgt ctgcaacact ggtttcctgt ggcttcgcgg cgggttttgc ccctttggcc
    46801 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    46861 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc
    46921 agttcaacgt cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt
    46981 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct
    47041 ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt
    47101 tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag cgccaggaaa
    47161 tcgatgatca gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    47221 gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    47281 tcgccagtgc tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    47341 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    47401 atcgcgtgca agggttcgat ttgtgctcgc agatcgtccg gcattggcgg gcgatgtgat
    47461 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    47521 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    47581 gcaccggtcg gttcgcctgc gctgttagtc agcggaggaa acgcgtgata tgcgcgataa
    47641 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    47701 gtgcctgttt atcagattat gggtaaagga tgccacagac aggataaaaa catgagtttt
    47761 tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg
    47821 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagt
    47881 atgatgtttg tctttgtttt tcatgcagtt aaaaataaag ccatccctaa taattgagat
    47941 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    48001 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
    48061 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    48121 gtatcgtatt gaccattttg gtcactattt tttgctccgt cccgatcatc attgccggga
    48181 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    48241 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
    48301 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    48361 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
    48421 tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct
    48481 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg
    48541 agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
    48601 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa
    48661 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
    48721 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
    48781 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc gtacatgtgg
    48841 atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc
    48901 gccattttca gcaatggctg aactcactgt ggcaggaaaa agaccggctt ctgacgtcgt
    48961 tgatgtcatc acagcggcat gacaagtaaa ttttgctcgc ctctgaatga gcagaggcga
    49021 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc
    49081 ctgtccgtta aaaacaactt tacccggtgt ataatgcagc tgcagcgagg cggtattgtt
    49141 gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga tttgtttatc
    49201 agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt
    49261 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga tatctttgtt
    49321 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
    49381 gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt tttgtaataa
    49441 cggtaaatac tctttgaaca atgcagcatt catttcttgc agagcaactt catcgtttgc
    49501 taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg
    49561 gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
    49621 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
    49681 gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg caacgtcttc
    49741 cccctgattg ataatattaa cctgattaaa tttcgactcg ctttcaccaa ccggaaattt
    49801 cttttcttct aatcgtgtca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
    49861 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
    49921 actaccatca ccattaaaag taaactggcc ctcaccgaaa gaaaactttc catactctgc
    49981 agaggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
    50041 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
    50101 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
    50161 caacggaccg tgctcaacat ccacatcaaa tgcaactttc tggcctggct tgatattgag
    50221 atcgggtgca ccgttatcaa atgaaatttg catttgaaaa tgggaattaa aaaagccttt
    50281 gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc
    50341 gttaaaatct ttaataaatt tttcgacacc aggctgaatt tgcgtacctg tgtaccatgt
    50401 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
    50461 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
    50521 cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa aaaagcccgt
    50581 gaatattcac gggctttatg taatttacat tgaattattt tttctcggac agatatttca
    50641 ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata ccctgcggat
    50701 tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc acgtcagctg
    50761 cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac gccgcgtcgt
    50821 actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa gcagaacgaa
    50881 tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct tccacgccca
    50941 gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca cccatgaagt
    51001 tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca ttatcagaaa
    51061 tatgcagtac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa aactccagca
    51121 cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca tcttcatact
    51181 gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa atctttttca
    51241 tgatctactc tctccgatta atacataggt gttaattgca aagggggttc ttgtagaact
    51301 tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc ggttaaccac
    51361 gggaaatttt tcgggaacgc gggatcagcc caacgccgca ttagccaggc aagataataa
    51421 accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc gaactcgcta
    51481 aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctcggc tttatcgcca
    51541 ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc cagatcaaca
    51601 aacattgggc catcgcgcca gagaatattc ccggcgtggc agtctccatg tagccgcaga
    51661 acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc tgtcgctttc
    51721 aggaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag cttgcgtggc
    51781 tcaatgagat attcattcag accgatggtc gggcgatggt taaaaagctg tttgcgcccc
    51841 gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat attatcagct
    51901 tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg atgattcaat
    51961 aaagtctgac cgttaaaggc cacaggtgct gcgaccggga cttcatcatt taccagctgc
    52021 aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg gcgataaaat
    52081 ttgacgacaa aacgtcgacg atcttcgtcc tgaaattgat agacacggtt ttcatagctg
    52141 ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc atccatgatg
    52201 gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc atccggaaaa
    52261 cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc ttacaagctt
    52321 ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt cttcataatc
    52381 tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca ggtgatagat
    52441 cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt tcgccagttt
    52501 ctgcaaaaat tgcagcaggt taaggtctgg ctctttttgc caggctggct ggaggagttc
    52561 aataacttca ttcagacgtt tacatttcat agtgatgctc cttactcttg agacagacac
    52621 gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat gacgacgata
    52681 acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga taaaggattg
    52741 cttgaattaa acggcaaacc attatggcaa catgtcgctg acgcgcttat gacgcagctc
    52801 tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg cggtctgaaa
    52861 gtgattgaag attcactggc ggattaccca ggccctctgg cagggatgct ttcagtaatg
    52921 cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta cattccccat
    52981 gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg ggtccatgac
    53041 ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc tttattacta
    53101 gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc tggcggtcat
    53161 gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc agaggagctt
    53221 gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg agtggtaccg
    53281 gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg atccgtccag
    53341 ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa gatagctatg
    53401 agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga tgggccttga
    53461 tgacggaaac accagacgaa gaagagctgg atctacagtt tctcgcaagt cgaatggata
    53521 cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc gcaaagattg
    53581 tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac aggcatgtta
    53641 ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat attaatgatg
    53701 ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa tcaggacgga taagcaagag
    53761 taggctggag cagacgcgcc agcgtcgcat ccggcatttt tttgcaccaa cgcaaaaagg
    53821 ccatccgtca ggatggcctc tcgctttatt tgatgcctgg cagttcccta ctctcgcatg
    53881 gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
    53941 aggtgggacc accgcgcta
//
LOCUS       DAZGCQ010000021        53609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000021 DAZGCQ010000000
VERSION     DAZGCQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.021"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            373..459
                     /gene="leuL"
                     /locus_tag="ACHK07_001350"
     CDS             373..459
                     /gene="leuL"
                     /locus_tag="ACHK07_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO8101706.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            552..2123
                     /gene="leuA"
                     /locus_tag="ACHK07_001351"
     CDS             552..2123
                     /gene="leuA"
                     /locus_tag="ACHK07_001351"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO8101707.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2123..3214
                     /gene="leuB"
                     /locus_tag="ACHK07_001352"
     CDS             2123..3214
                     /gene="leuB"
                     /locus_tag="ACHK07_001352"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO8101708.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYY
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASTIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3217..4617
                     /gene="leuC"
                     /locus_tag="ACHK07_001353"
     CDS             3217..4617
                     /gene="leuC"
                     /locus_tag="ACHK07_001353"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO8101709.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4628..5233
                     /gene="leuD"
                     /locus_tag="ACHK07_001354"
     CDS             4628..5233
                     /gene="leuD"
                     /locus_tag="ACHK07_001354"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO8101710.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5341..5472)
                     /gene="sgrT"
                     /locus_tag="ACHK07_001355"
     CDS             complement(5341..5472)
                     /gene="sgrT"
                     /locus_tag="ACHK07_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO8101711.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5561..7219
                     /gene="sgrR"
                     /locus_tag="ACHK07_001356"
     CDS             5561..7219
                     /gene="sgrR"
                     /locus_tag="ACHK07_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO8101712.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7448..8017)
                     /gene="espY2"
                     /locus_tag="ACHK07_001357"
     CDS             complement(7448..8017)
                     /gene="espY2"
                     /locus_tag="ACHK07_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO8101713.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8259..8358
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8389..9372
                     /gene="thiB"
                     /locus_tag="ACHK07_001358"
     CDS             8389..9372
                     /gene="thiB"
                     /locus_tag="ACHK07_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO8101714.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9348..10958
                     /gene="thiP"
                     /locus_tag="ACHK07_001359"
     CDS             9348..10958
                     /gene="thiP"
                     /locus_tag="ACHK07_001359"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO8101715.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10942..11640
                     /gene="thiQ"
                     /locus_tag="ACHK07_001360"
     CDS             10942..11640
                     /gene="thiQ"
                     /locus_tag="ACHK07_001360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO8101716.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSIVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(11727..12491)
                     /gene="yabI"
                     /locus_tag="ACHK07_001361"
     CDS             complement(11727..12491)
                     /gene="yabI"
                     /locus_tag="ACHK07_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8101717.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12577..13455)
                     /gene="araC"
                     /locus_tag="ACHK07_001362"
     CDS             complement(12577..13455)
                     /gene="araC"
                     /locus_tag="ACHK07_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO8101718.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13794..15494
                     /gene="araB"
                     /locus_tag="ACHK07_001363"
     CDS             13794..15494
                     /gene="araB"
                     /locus_tag="ACHK07_001363"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO8101719.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHMP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15505..17007
                     /gene="araA"
                     /locus_tag="ACHK07_001364"
     CDS             15505..17007
                     /gene="araA"
                     /locus_tag="ACHK07_001364"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO8101720.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17176..17871
                     /gene="araD"
                     /locus_tag="ACHK07_001365"
     CDS             17176..17871
                     /gene="araD"
                     /locus_tag="ACHK07_001365"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8101721.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17946..20297
                     /gene="polB"
                     /locus_tag="ACHK07_001366"
     CDS             17946..20297
                     /gene="polB"
                     /locus_tag="ACHK07_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO8101722.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20461..23367
                     /gene="rapA"
                     /locus_tag="ACHK07_001367"
     CDS             20461..23367
                     /gene="rapA"
                     /locus_tag="ACHK07_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO8101723.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISVLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23379..24038
                     /gene="rluA"
                     /locus_tag="ACHK07_001368"
     CDS             23379..24038
                     /gene="rluA"
                     /locus_tag="ACHK07_001368"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO8101724.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24156..24971)
                     /gene="djlA"
                     /locus_tag="ACHK07_001369"
     CDS             complement(24156..24971)
                     /gene="djlA"
                     /locus_tag="ACHK07_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO8101725.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGDNWQQTQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25226..27580
                     /gene="lptD"
                     /locus_tag="ACHK07_001370"
     CDS             25226..27580
                     /gene="lptD"
                     /locus_tag="ACHK07_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO8101726.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27633..28919
                     /gene="surA"
                     /locus_tag="ACHK07_001371"
     CDS             27633..28919
                     /gene="surA"
                     /locus_tag="ACHK07_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO8101727.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28919..29908
                     /gene="pdxA"
                     /locus_tag="ACHK07_001372"
     CDS             28919..29908
                     /gene="pdxA"
                     /locus_tag="ACHK07_001372"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO8101728.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAILGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLSGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29905..30726
                     /gene="rsmA"
                     /locus_tag="ACHK07_001373"
     CDS             29905..30726
                     /gene="rsmA"
                     /locus_tag="ACHK07_001373"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO8101729.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30729..31106
                     /gene="apaG"
                     /locus_tag="ACHK07_001374"
     CDS             30729..31106
                     /gene="apaG"
                     /locus_tag="ACHK07_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO8101730.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31113..31961
                     /gene="apaH"
                     /locus_tag="ACHK07_001375"
     CDS             31113..31961
                     /gene="apaH"
                     /locus_tag="ACHK07_001375"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO8101731.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(31958..32272)
                     /locus_tag="ACHK07_001376"
     CDS             complement(31958..32272)
                     /locus_tag="ACHK07_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO8101732.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(32275..32508)
                     /locus_tag="ACHK07_001377"
     CDS             complement(32275..32508)
                     /locus_tag="ACHK07_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO8101733.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(32594..33073)
                     /gene="folA"
                     /locus_tag="ACHK07_001378"
     CDS             complement(32594..33073)
                     /gene="folA"
                     /locus_tag="ACHK07_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO8101734.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33265..35127)
                     /gene="kefC"
                     /locus_tag="ACHK07_001379"
     CDS             complement(33265..35127)
                     /gene="kefC"
                     /locus_tag="ACHK07_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO8101735.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(35120..35650)
                     /gene="kefF"
                     /locus_tag="ACHK07_001380"
     CDS             complement(35120..35650)
                     /gene="kefF"
                     /locus_tag="ACHK07_001380"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO8101736.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLDGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGKSHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35761..37092)
                     /gene="yaaU"
                     /locus_tag="ACHK07_001381"
     CDS             complement(35761..37092)
                     /gene="yaaU"
                     /locus_tag="ACHK07_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101737.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(37150..37437)
                     /gene="fixX"
                     /locus_tag="ACHK07_001382"
     CDS             complement(37150..37437)
                     /gene="fixX"
                     /locus_tag="ACHK07_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO8101738.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37434..38720)
                     /gene="fixC"
                     /locus_tag="ACHK07_001383"
     CDS             complement(37434..38720)
                     /gene="fixC"
                     /locus_tag="ACHK07_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8101739.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38771..39712)
                     /gene="fixB"
                     /locus_tag="ACHK07_001384"
     CDS             complement(38771..39712)
                     /gene="fixB"
                     /locus_tag="ACHK07_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8101740.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGTDGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39727..40497)
                     /gene="fixA"
                     /locus_tag="ACHK07_001385"
     CDS             complement(39727..40497)
                     /gene="fixA"
                     /locus_tag="ACHK07_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO8101741.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40970..42484
                     /gene="caiT"
                     /locus_tag="ACHK07_001386"
     CDS             40970..42484
                     /gene="caiT"
                     /locus_tag="ACHK07_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO8101742.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42515..43657
                     /gene="caiA"
                     /locus_tag="ACHK07_001387"
     CDS             42515..43657
                     /gene="caiA"
                     /locus_tag="ACHK07_001387"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO8101743.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43786..45003
                     /gene="caiB"
                     /locus_tag="ACHK07_001388"
     CDS             43786..45003
                     /gene="caiB"
                     /locus_tag="ACHK07_001388"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO8101744.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            45077..46630
                     /gene="caiC"
                     /locus_tag="ACHK07_001389"
     CDS             45077..46630
                     /gene="caiC"
                     /locus_tag="ACHK07_001389"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO8101745.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPKFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46739..47524
                     /gene="caiD"
                     /locus_tag="ACHK07_001390"
     CDS             46739..47524
                     /gene="caiD"
                     /locus_tag="ACHK07_001390"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO8101746.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVDSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47530..48120
                     /gene="caiE"
                     /locus_tag="ACHK07_001391"
     CDS             47530..48120
                     /gene="caiE"
                     /locus_tag="ACHK07_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO8101747.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVAPKR"
     gene            complement(48239..48634)
                     /gene="caiF"
                     /locus_tag="ACHK07_001392"
     CDS             complement(48239..48634)
                     /gene="caiF"
                     /locus_tag="ACHK07_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO8101748.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48895..52116)
                     /gene="carB"
                     /locus_tag="ACHK07_001393"
     CDS             complement(48895..52116)
                     /gene="carB"
                     /locus_tag="ACHK07_001393"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO8101749.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(52134..53282)
                     /gene="carA"
                     /locus_tag="ACHK07_001394"
     CDS             complement(52134..53282)
                     /gene="carA"
                     /locus_tag="ACHK07_001394"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO8101750.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETAIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        12818 a        14453 c        14096 g        12242 t
ORIGIN      
        1 tttatttata tgtttaatgc attaattctt aacattaatt gatcaataat attcaccaaa
       61 tcaatatcaa aaaatccgca aaacatataa ttcaatacaa atcatcagga taggttttgc
      121 aacgcgtgca ttttgtcccc tttttcctcg tggattagat gcaaaaattt attctgaaat
      181 atgtcaaccg atgaaaagcg tcggtagtta atcagaaatt aatatcgctt gttttaacca
      241 cagcagcaca attagctaat tttacggatg cagaactcat gctggcggga cgtttttatt
      301 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      361 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      421 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      481 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa
      541 ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      601 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      661 gtatgggtgt tgacgtgatg gaagtcgggt tccccgtctc ttcgccgggt gattttgaat
      721 cagtgcaaac catcgctcgc caggttaaaa acagccgcgt atgcgcgtta gctcgctgcg
      781 tggagaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc
      841 atacctttat tgccacttcg ccaatgcaca tcgccaccaa gctgcgcagc acgctggacg
      901 aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      961 aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag
     1021 cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
     1081 cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtgcctaac atcgacaaag
     1141 ccattatttc cgtacatacc cacgacgatt tgggcctggc ggtcggcaac tcactggcgg
     1201 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1261 gaaactgttc actggaagaa gtcatcatgg cgatcaaagt tcgtaaggat attctcaacg
     1321 tccacaccgc catcaatcac caggagatat ggcgcaccag ccagttagtt agccagattt
     1381 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct
     1441 ccggtatcca ccaggatggt gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1501 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1561 tgaaacatcg catggatgag atggggtata aagaaagtga atataattta gacaatttgt
     1621 acgacgcctt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1681 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1741 gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc tgcggcgaag
     1801 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcgatta
     1861 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgct aaaggtcacg
     1921 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg
     1981 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg catgtactga
     2041 acaatatctg gcgcgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     2101 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2161 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2221 ctttgcgatg cgcatcacca ccagccatta ttatgtaggc ggcgcagcca ttgataacca
     2281 cgggcagccg ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2341 tggctcggta ggcggtccga agtgggaaca tttaccacca gaccagcaac cagaacgcgg
     2401 cgcgctgctg cctctgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact
     2461 gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcaa acggcttcga
     2521 catcctgtgt gtgcgtgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2581 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagattga
     2641 acgtatcgcc cgcatcgcat ttgaatctgc tcgcaagcgt cgccacaaag tcacctcaat
     2701 cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc
     2761 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2821 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2881 gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa
     2941 cgagcaaggt tttggactgt atgaaccggc gggcggctcg gcaccagata tcgcaggcaa
     3001 aaacatcgcc aacccgattg cacaaatcct gtcgctggca ctgctgctgc gttacagcct
     3061 ggatgccgat gatgcggctt ccaccattga acgcgccatt aaccgcgcat tagaagaagg
     3121 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3181 tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaagacgtt atacgaaaaa
     3241 ttgtttgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3301 cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt
     3361 cgcccggtac gtcagccggg gaaaaccttc gccaccatgg atcacaacgt ttcaactcag
     3421 accaaagaca ttaatgcctg cggtgaaatg gcgcgcatcc agatgcagga gctgatcaaa
     3481 aactgcaaag aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc
     3541 cacgtgatgg ggccggaaca gggcgtgacc ttgccgggga tgaccattgt ctgcggcgac
     3601 tcgcacaccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3661 gttgaacacg tactggcaac gcaaaccctg aaacagggcc gtgcgaagac catgaaaatt
     3721 gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgtt ggcaattatc
     3781 ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3841 cgtgatttaa gcatggaagg tcggatgacc ctgtgcaata tggcaatcga aatgggcgcg
     3901 aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg tcgtctgcat
     3961 gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac
     4021 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagagatttc gccgcaggtc
     4081 acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccagct
     4141 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4201 aaaccgggta ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc
     4261 aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaaggccg gaaagtcgcg
     4321 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4381 gaaggtctgg acaaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4441 atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc ctccaccagc
     4501 aaccgtaact ttgaaggccg tcaggggcgc ggcgggcgca cgcatctggt cagcccggca
     4561 atggctgccg ccgcggctgt gaccggccat ttcgccgaca ttcgcaacat taaataagga
     4621 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4681 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4741 ttttggcgcg catctgttta acgactggcg ttttctggat gaaaaaggcc aacagccaaa
     4801 cccggacttc gtgctgaact tcccgcagta tcagggggct tccattttgc tggcgcggga
     4861 aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt
     4921 taaggtggtg attgcgccga gttttgctga catcttctat ggcaatagct ttaacaacca
     4981 gctgctgccg gtgaaattaa gcgacgcaga agtggatgag ctgtttgcgc tggtgaaagc
     5041 caatccgggg attcattttg atgtagacct ggaagcgcaa gaggtgaaag cgggtgagaa
     5101 aacctatcgc tttaccattg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5161 cattgggctt accttgcagc acgacgacgc cattgcggct tatgaagcga agcaacccgc
     5221 gtttatgcgt tagtttgtac accgtccggt actcaatcgc caaaaagaaa aaagccagca
     5281 gataatcact gctggcgtgg cgattaacac caatactcag tcacacatga tgtccgcaaa
     5341 tcagtagtca gaatgactcc cctcccactg catcagttct tcaagcatgg ttaatcgttg
     5401 tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat agtgctgata
     5461 aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact taatataggg
     5521 aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg ctcgtctgca
     5581 acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa
     5641 cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgcaccctgc tcaacaccat
     5701 gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgtggtaaac gctcgcgtct
     5761 gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc tgctggagca
     5821 ggaccgtatc gaccaactag tgcagttagt tggcgacaaa gcgactgtga ggcaaatgct
     5881 ggtttctcat ctgggccgta gcttccgcca ggggcggcac atcctgcgcg tgctctacta
     5941 tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa cccatatcgc
     6001 ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac tggaagcaga
     6061 catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt tgcgtccagg
     6121 agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct ctttaaaacg
     6181 aatcaatacg ctgccgctct attcgcatat tgctgacatt gtgtcgccga cgccctggac
     6241 gctggatatc cacctcacgc agccggatcg ctggttgccg ttactgctgg gacaagttcc
     6301 ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc atcccatcgg
     6361 caccggtccg tatgcggtga ttcgcaacag caccaatcaa ctgaaaattc aggcattcga
     6421 tgacttcttc ggttaccggg cattaatcga tgaagttaat gtctgggttc tgccggaaat
     6481 tgccgacgag ccagccggag ggctgatgct gaaaggccca cagggcgagg aaaaagagat
     6541 tgaaagccga ctggaggaag gttgttacta tttactgttc gacagccgaa cccatcgcgg
     6601 ggcgaatcaa caagtcagag actggataag ctatgtgctt tcgccgacca atctggtcta
     6661 tttcgccgag gaacagtacc agcaactgtg gtttccggcc tatgggctgc tcccccgctg
     6721 gcaccacgcc cgcccaacac attgcgaaaa acctgccggg ctggaacatc tcaccctgac
     6781 gttttatcag gatcatattg aacatcgggt gattgccagg atcatgcagc agattctggc
     6841 gagtcatcag gtcacgatgg aaatcaaaga gatcagctac gatcaatggc acaaaggtga
     6901 gatcgaaagc gacatatggc ttaacagcgc caactttacg ctgccgctgg atttttcgct
     6961 gttcgcgcac ttgtgcgaag tacccctgct gcaacactgt attccgctcg actggcaagc
     7021 cgacgcagcc cgctggcgca atggcgagat aaatctggcg aactggtgcc agcaactggt
     7081 cgccagtaaa gcaatggtgc cacttatcca ccactggctg atcattcagg ggcaacgcag
     7141 tatgcgcggc ctgcgcatga ataccctcgg ctggttcgat tttaaatcag cgtggtttgc
     7201 gccgccggat ccagagtagc ttgcgccttt tggcggtcat gagcgcattt tttatattca
     7261 gacggaatga taaaatacat tacttttata ttttctctgt tttatgcatc gtctgattag
     7321 cgtcgcaaat aagattaaag aaggggcatt ccagcccctc tttcatattc tgagttaatc
     7381 aataacggcg cagtctagcc ttgttctggc cggaaagatc atctttttca ggttaacctc
     7441 gcgcaggtca gtcataccaa cggctattgt tcgtttcagc attgctcagg tcaacctcga
     7501 gacagtcaat accacaaatg acaaccccgt taaagtaagc attactcaga tcaatgcacc
     7561 cggtcctggt gttctctttc agctgcaact tcagagtaaa tatcgcctca tcgccaataa
     7621 aaaaactgac gcataacgta tcttttggcg agagtgaggc cttcgcggcc atattttccg
     7681 gatctcttgc gtgttctttg agttcattaa attttatcag ccagtgagtt aactgatcac
     7741 gcgccttccc gtcgacagcc ccggtaaagt attttgagta gtcatcacta ttctggaaat
     7801 aaaatgcatc cttcagaatt tgccttgccg cagtctggga atgtttatct gaccggaaga
     7861 gattgcgtaa cgtctcccat agctcttcaa agatattcat attctcggtc tttttgacca
     7921 agttagccat cctgataatc tgagaattag taaggcctgg atctttagtg gtattactag
     7981 cagggatact aatctgttct gggtttctta ctttcatgct gatagctcca ctctgagtaa
     8041 aaaactcaga ataagcgtcc tgaataagaa tactgtcaaa aatcgctcat tcacgcaata
     8101 aacggttacg ttacctattt tgactgtagc gaagggattc tttcggatca acgctctcca
     8161 gctatgcaaa tacgctcttg atagctgaac gaatattggc ttttttaata cgccttctct
     8221 atggattgct ggttgataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg
     8281 cggacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga
     8341 tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa
     8401 tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt
     8461 tatacctacg attccttcgc cgccgactgg gggccaggtc cggtggttaa aaaagccttt
     8521 gaagccgact gtaattgcga actgaaactg gtggcgctgg aagatggcgt ttcgcttctc
     8581 aaccgtctac ggatggaagg caaaaacagt aaagccgatg tggtgctggg gctggataac
     8641 aacctgttag acgccgccag taaaaccgga ctgtttgcca aaagcggtgt ggcagcggat
     8701 gccgttaacg ttcccggcgg ctggaataat gacactttcg taccgtttga ttatggctac
     8761 ttcgccttcg tttatgacaa gaacaaactg aaaaacccgc cacaaagcct gaaagaactg
     8821 gttgagagcg atcaaaactg gcgggtgatt tatcaggatc cgcgcaccag tacaccgggg
     8881 ctgggtctgt tgctatggat gcaaaaagtc tatggcgatg acgccccaca agcctggcag
     8941 aaactggcga agaaaaccgt caccgtcacc aaaggctgga gcgaagccta cggcctgttt
     9001 ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc
     9061 gaagagaaga aagataacta cgccgccgcg aacttcagcg aaggtcacta tctgcaagtg
     9121 gaagtcgccg ctcgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag
     9181 tttatggttt ctccggcttt ccagaatgcg atcccaaccg gcaactggat gtatccggtg
     9241 gcaaacgtca cgctgcctgc cggttttgaa aaattgacca aacccgcaac cacgctggag
     9301 ttcacgccag ccgaagtggc ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc
     9361 gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtgatag
     9421 cggttgcgct ggcggcgttt ctcgccctgt ggtcgaacgc gccgcagggt gactggtcgg
     9481 cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc
     9541 tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgcgctc tatcgcaggc
     9601 gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc
     9661 tggttgctgt tttcggcatt cttagcgtct atggtcgcca gggctggctg gcatcgctct
     9721 gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggt attttgctgg
     9781 cgcacgtatt ttttaatcta ccgatggcga gccgcttatt actccaggca ctggaaaaca
     9841 ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc
     9901 gcttcgtcga atggccgtgg ttacggagac aaatcccgcc ggttgctgcg cttatcttta
     9961 tgctctgttt cgccagcttc gccaccgtac tgtcgctggg cggcggtccg caggcgacca
    10021 ctatcgagct ggcaatctat caggcgctga gttacgacta cgatcctgcc cgcgcggcga
    10081 tgctggcgct gatccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactga
    10141 gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgacccg gacgatcgtc
    10201 tgcatagccg catttgcgac acggtgttaa ttgtgctggc gctgctgctg ttgcttccac
    10261 cgttactggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac
    10321 cggtactgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggcgtattgt
    10381 gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa
    10441 tgctggcggg tcaggcgctg gagatgagtg gcatgttgat cctcgccatg ccggggattg
    10501 tgctggcaac gggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg
    10561 acggcattgt gattttcacc aatgcgttaa tggcgatccc ctacgccctg aaagtgctgg
    10621 aaaacccgat gcgcgatatc accgcccgct acagcatgtt atgtcagtcg ctggggattg
    10681 aaggctggtc acgcttaaaa gtggtggagc tgcgcgccct gaaacgtcca ctggcgcagg
    10741 cgctggcctt tgcctgcgtg ctgtcgattg gtgattttgg cgtggtagcg ttgttcggta
    10801 acgatgattt ccgcaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc
    10861 aggacggcgc ggtcaccgcg ttaattctgc tgctactctg ttttctgctg tttaccgtga
    10921 ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct ttaccaccat
    10981 ttgccgatgc gttttagctt aacagtagaa cgcggcgagc aggtggcgat cctcgggcca
    11041 agcggcgcag gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc
    11101 ggttcgctga ctatcgatgg cgttgatcac accacgacgc cgccgtcacg ccgtccggtg
    11161 tcgatgctgt ttcaggagaa caacctgttc agccacctga cggtcgcgca gaacatcggg
    11221 ctggggctaa atccgggatt gaaactgaac gcggcacagc aggagaaaat gcacgcgatt
    11281 gcccgtcaga tggggattga taatttaatg gcgcggttac ctggcgagct ttccggcggt
    11341 cagcgacagc gagtggcgtt agcgcgttgt ctggtacgcg aacagccgat tttattgctc
    11401 gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgttgac gctggtgagc
    11461 acgagctgcc agcagcaaaa aatgacgtta ttgatggtgt cgcacagtgt ggaagatgcg
    11521 gcaaggattg caacacgctc gatagtggtc gccgacgggc gcatcgcctg gcagggtaag
    11581 accgaagaat tgttgagcgg taaggcgagt gcttcggcgc tgttgggaat tacaggttaa
    11641 tatgcactgt cggatgcggc gtgaacgcct tatccgacct acgggtgcgt tttgtccgca
    11701 tcaagcgccc cacacaagac tatgccctaa actccaacca ctttacgcaa aatatcgata
    11761 tacaccggca ttaacggatg gcgaattaac accaccagcg ccaccacgcc gatggcagaa
    11821 atcaacggcg tcagccacaa caaacgaccg cgggacaaat aatgactcaa acggtcagtc
    11881 gctttaccac tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc
    11941 gtcgccagca gcaaccattt aaactcaccg ctctgcattc cggctggaat atcgatcgcc
    12001 gcgcccgcca gaatccctgg caggaagtaa aacggcggcc acagcaggca gccgataata
    12061 ttcggcgtaa taaatttagc caccggcaga tccagcattc ccgccaccat tggcaccagc
    12121 ggacgcgtcg ggccaacaaa acgaccgacc agaatggtga acatgctgtg ttgatgcaac
    12181 gcatgttcag ttttatcgag taatgctttg ttcttcttca gaaatgacca gcgatgcaat
    12241 ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag acaaccgacg
    12301 atccctgcca gccaggcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc
    12361 cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag
    12421 gccaccaaca ccaccgccat caatgaatac acggtggatt gggtaataaa gtgttccagc
    12481 aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
    12541 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
    12601 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
    12661 aaatactcgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
    12721 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atgcgttggt cctcgcgcca
    12781 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
    12841 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
    12901 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
    12961 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
    13021 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
    13081 cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc
    13141 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
    13201 atgacggccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
    13261 gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
    13321 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
    13381 cggcgttaaa cccgccacca gatgggcgtt aaacgagtat cccggcagca ggggatcatt
    13441 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
    13501 ttgcatcaga tattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt
    13561 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
    13621 taacaaaagt gtctataatc acggcagaaa agtccacatt gataatttgc acggcgtcac
    13681 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
    13741 ttcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga
    13801 ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca
    13861 gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt
    13921 gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag
    13981 cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga
    14041 ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg
    14101 cgctgcgccc agagtttgcc gaaaacccga acgcgatgtt cgtattgtgg aaagaccaca
    14161 ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact
    14221 actcccgcta cattggcggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg
    14281 tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact
    14341 gggtgccagc tctgctttcc ggtaccacgc gcccgcagga tattcgtcgc ggacgttgca
    14401 gcgccgggca taagtcgcta tggcacgaaa gctggggcgg cctgccgcca gccagtttct
    14461 ttgacgaact ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgatacct
    14521 ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc
    14581 ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggtgcag
    14641 gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg
    14701 ccgacaaaca gagcgttggc gagcgggcag tgaaaggtat ttgcggtcag gttgatggca
    14761 gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt gatatctacg
    14821 cctggttcgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag catccggaac
    14881 taaaagagca aattaacgcc agccagaaac aactgcttcc ggcgctgacc gaagcatggg
    14941 ccaaaaatcc gtctctggat cacatgccgg tggtgctcga ctggtttaac ggccgccgca
    15001 caccgaacgc taaccaacgc ctgaaagggg tgattaccga ccttaacctc gctaccgatg
    15061 ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg
    15121 agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc
    15181 ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg
    15241 ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag
    15301 tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag aaaaccctgc
    15361 aaccgtgcag cgagcaggca caacgctttg aacagcttta tcgccgctat cagcaatggg
    15421 cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct gcccaggccg
    15481 ttccgactct ataaggacac gataatgacg atttttgata attatgaagt gtggtttgta
    15541 attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag
    15601 cacgttgtta atgcgctaaa tacggaagcg aaactgccct gcaaactggt gttgaaaccg
    15661 ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta cgacgatcgt
    15721 tgcgctggtc tggtggtatg gctgcacacc ttctccccgg ccaaaatgtg gatcaacggc
    15781 ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc ggcgctgccg
    15841 tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag
    15901 ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tggttaccgg tcactggcag
    15961 gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtctc taaacaggat
    16021 acccgtcatc tgaaagtctg ccgttttggc gataacatgc gtgaagtggc ggtcaccgat
    16081 ggcgataaag ttgccgcaca aatcaagttc ggtttctccg tcaatacctg ggcggttggc
    16141 gatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag
    16201 tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgacagaac
    16261 gtgctggaag cggcgcgtat tgagctgggg atgaaacgtt tcctggaaca aggtggcttc
    16321 cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc aggtctggcc
    16381 gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc
    16441 gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg
    16501 gaggactaca cctatcactt cgagaaaggc aatgacctgg tgctcggctc ccacatgctg
    16561 gaagtctgcc cgtcgatcgc cgcagaagag aaaccgatcc tcgacgttca gcatctcggt
    16621 attggtggta aggacgatcc tgcccgcctg atcttcaata cccaaaccgg cccagcgatt
    16681 gtcgccagcc tgattgatct cggcgatcgt taccgtctac tggttaactg catcgacacg
    16741 gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcgctgtg gaaagcgcaa
    16801 ccggatctgc caactgcttc cgaagcgtgg atcctcgcag gtggcgcgca ccataccgtc
    16861 ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca cgacattgaa
    16921 atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcgct gcgctggaac
    16981 gaagtgtatt acggatttcg tcgctaagta gccgcatcag gtgtgtaacg cctgatgcga
    17041 cgctgacgcg tcttatcagg cctacacgct gtgattttgt aggccggata agcaaagcgc
    17101 atccggcatt gttttccccc tcgccccttt ggggagaggg ccggggtgag gggaaacgac
    17161 ctctcaggag tcaacatgtt agaagatctc aaacgccagg tattagaagc caatttggcg
    17221 ctgccaaaac ataacctggt cacgctcacc tggggcaacg tcagcgccgt tgatcgcgag
    17281 cgcggcgtct ttgtgatcaa accttccggc gtcgattaca gcatcatgac cgccgacgat
    17341 atggtcgtgg tcagcatcga aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc
    17401 gacacgccaa ctcaccggct gctctatcag gcattcccgt ccattggcgg cattgtgcac
    17461 acacactcgc gccacgccac catctgggcg caggcgggtc agtcgattcc agcaactggt
    17521 accacccacg ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca
    17581 gaaatcaacg gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac cttcgaaaaa
    17641 cagggtatcg atgcagcgca aatgcccggc gttctggtcc attctcacgg cccatttgca
    17701 tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat
    17761 atggggatat tctgccgtca gttagcgccg cagttaccgg atatgcagca aacgctgctg
    17821 gataaacact atctgcgtaa gcatggcgcg aaggcatatt acgggcagta atgactgtat
    17881 aaaaccacag ccaatcaaat gaaaccaggc tataatcaag cctggttttt tgatggattt
    17941 tcagcgtggc gcaggcaggt tttatcttaa cccgacactg gcgggatacc ccgcaaggaa
    18001 cagaggtctc cttctggctg gcgacggaca acgggccgtt gcaggttttc cttgcgccgc
    18061 aagagtccgt ggcgtttatt cccgccgatc aggttccccg cgctcagcat attttgcggg
    18121 gtgaacaagg ctttcgcctg acgccgctgg cgttaaagga ttttcaccgc cagccggtgt
    18181 atggccttta ctgtcgcgcc catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag
    18241 gtggcgttac cgtctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt
    18301 ttatcacctc gccggtgtgg gtcgagggtg atatgcacaa tggcgctatc gttaatgccc
    18361 gtctgaaacc gcatcccgac tatcgcccgc cgctcaagtg ggtgtctatt gatattgaaa
    18421 ccacccgcca cggtgagctg tattgcatcg gcctggaggg ctgcgggcag cgcatcgttt
    18481 atatgctggg gccggagaat ggcaacgctt ccgcgctcga tttcgaactg gaatacgtcg
    18541 ccagtcgccc gcagctactg gaaaaactta acgcctggtt tgccaactac gatcctgatg
    18601 tgatcatcgg ttggaacgtg gtgcaattcg atctgcgaat gctgcaaaaa catgccgagc
    18661 gttatcgtat tccgctgcgg ctggggcgcg ataacagcga gctggagtgg cgcgagcacg
    18721 gctttaaaaa cggcgtcttt tttgcccagg ctaaagggcg gctaattatc gacggtatcg
    18781 aggcgctgaa atccgcgttc tggaatttct cttcattctc gctggaaact gtcgctcagg
    18841 agctattagg cgaaggaaaa tctatcgata acccgtggga tcgaatggac gaaattgacc
    18901 gccgtttcgc cgaagataaa cctgcgctgg cgacttataa cctgaaagat tgcgagctgg
    18961 tgacgcagat tttccataaa actgaaatca tgccgttttt gctcgaacga gcaacggtga
    19021 acggcctgcc ggtagaccgc cacgggggat cggtggcggc gtttggtcat ctctattttc
    19081 cccggatgca tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca
    19141 gccctggcgg ctacgtgatg gattcgcgac ctgggcttta tgattcggtg ctggtgctgg
    19201 actacaaaag cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcgggctgg
    19261 tggaaggcat ggcgcagcct gatccagagc acagcaccga aggctttctc gatgcctggt
    19321 tctcgcgaga aaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg
    19381 aagccaaacg tcagggtaat aagccgcttt cgcaggcgct gaagatcatt atgaatgcct
    19441 tttatggcgt gctcggcacc accgcctgcc gcttcttcga tccgcggctg gcatcgtcga
    19501 tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc gttgattgaa gcgcagggct
    19561 acgacgtgat ctacggcgat actgactcaa cgtttgtctg gctgaaaggc gcacattcgg
    19621 aagaagaagc ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg
    19681 aaacgctgca aaaacaacag ctgaccagcg cactggaact ggagtatgaa acccatttct
    19741 gccgttttct gatgccaacc attcgcggag ccgataccgg cagtaaaaag cgttatgccg
    19801 ggctgattca ggagggcgac aaacagcgga tggtgtttaa agggctggaa accgtgcgta
    19861 cggactggac gccactggcc cagcagtttc agcaggagct ttacctgcgt atcttccgca
    19921 acgagccata tcaggagtac gtccgcgaaa ccatcgacaa actgatggcg ggtgaactgg
    19981 atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat cagcgtaatg
    20041 ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc
    20101 ccttgcaata ccagaaccgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc
    20161 cgctggacta ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac
    20221 ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc
    20281 aacttgggct attttgagca aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
    20341 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
    20401 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
    20461 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
    20521 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
    20581 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc
    20641 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
    20701 ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt
    20761 ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gcctgtttgc cgggcagatt
    20821 gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc
    20881 cgtatgccgt acagcggctt gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac
    20941 atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt
    21001 ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct
    21061 gaacgtgtgc tgattattgt cccggaaacc ttacagcatc agtggctggt agaaatgctg
    21121 cgccgtttca acctgcgttt tgcgctgttt gatgatgagc gttatgccga agctcagcac
    21181 gatgcttaca acccgttcga taccgaacaa ctggtgattt gctcgctgga ttttgcccgt
    21241 cgtagcaaac agcggctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
    21301 gaagcccatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa
    21361 caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg
    21421 atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg
    21481 cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg
    21541 gcaggtaata aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag
    21601 gatatcgagc cgctgttgca agcagcaaac agcgacagcg aagatgccca gagcgcccgt
    21661 caggaactgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
    21721 acccgtaacg gtgtgaaagg cttcccgaaa cgcgagttgc acaccattaa gctgccgcta
    21781 ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg
    21841 gaagatcgtg ctcgagatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat
    21901 aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc
    21961 catcgctctc agaaagtgct ggtgatctgc gctaaagctg ccactgcgct gcaactggag
    22021 caagtactgc gcgaacgtga aggtatccgc gctgctgtgt tccacgaagg catgtcgatt
    22081 attgagcgtg accgcgctgc cgcctggttt gctgaagaag acaccggcgc acaggtactg
    22141 ctgtgctcgg aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg
    22201 tttgacctgc cattcaaccc ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc
    22261 ggtcaggcgc acgatattca gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg
    22321 ctggtgcgct ggtatcacga aggtctggat gcattcgagc acacctgccc gaccggacgc
    22381 actatttacg atagcgtcta caacgatctg attaactatc tggcttcacc ggatcaaacc
    22441 gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacaa
    22501 ctggaacagg ggcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag
    22561 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg
    22621 aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg
    22681 accccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc
    22741 atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg cacagtttat tacctgggag
    22801 catccgctga tccgcaacgg tctggatctg atcctttctg gcgatactgg tagcagcacg
    22861 atttcagtgt tgaaaaacaa agcgttaccg gtaggtacgc tgttggtgga actgatttac
    22921 gtggtcgaag cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
    22981 gtacgtatgc tgctggataa aaatggcaac aacctggcgg cccaggtgga gtttgaaacc
    23041 tttaaccgcc agcttaacgc ggtcaaccgt cacaccggca gcaaactggt taatgccgtg
    23101 cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa atcggcccgt
    23161 gcattgattg ataccgcacg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt
    23221 ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg acgaactgac cgccattgaa
    23281 agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gctggcgtct ggatgccctg
    23341 cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc
    23401 caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg gtggtcaaca
    23461 agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagac agcgtgatga
    23521 cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca
    23581 gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc
    23641 gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg
    23701 aaggtctggt agatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct
    23761 gttacgaaac aggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata
    23821 acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata
    23881 tgctggcgct gggtcatccg attctcggcg atcgttttta tgcatcaccg gaagcgagag
    23941 cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg
    24001 gcaatagtat gacgtttaaa gcgccagcgg atttttaata gtcacactgg cgaagttcgg
    24061 ttggccggag aaggcgttca cgccgcatcc ggcaattttg cgccgattgc ctgatgcgac
    24121 gcgggccgcg tcttatcagg cctacagttt gaaacttatt taaacccttt ctgctgctta
    24181 atcaattcat aggcctgctg aatttcctgc gctttttgct tcgccatctc catcatctca
    24241 ggcggcaaac ctttcgccac caacttatcg ggatggtgtt cactcatcag cttacggtag
    24301 gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt acaggcatct
    24361 tccagcgtcg ggccacgctg ggtttgttgc cagttatcac cgccagattg ctgctgataa
    24421 ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg
    24481 gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg gtgcagtgaa
    24541 ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa gtcaaaacga
    24601 ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt
    24661 cccacccgga acgcgttttg cgccgcagta cgggaagcgc catgaaggtt cattcggtcc
    24721 atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga tttggttaaa
    24781 tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg ctggttggca
    24841 aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa caggcctaac
    24901 actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat gatttttccc
    24961 caatactgca tatattcccc aaatcgacac acggatatca gggctatctc ccacaatata
    25021 aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta taatttgcat
    25081 tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg acaggattaa
    25141 cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc
    25201 gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc tggccaccat
    25261 gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct cacagtgcat
    25321 gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg acttacccgt
    25381 gactatcaat gctgaccacg cgaaagggga ctacccggat gacgccgtgt ttactggcag
    25441 cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc tccatcaaaa
    25501 agaggcacca ggacaaccgg agccggtacg taccgttgat gcgctcggta atgtccatta
    25561 cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga
    25621 taccaacgtc tgggagggtg attaccagat ggtgggtcgc cagggccgcg gtaaagcgga
    25681 cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta gctttacctc
    25741 ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc atgaccgcga
    25801 agaacaagtg gccgaaatct ggaacgcccg ttttaaagtt ggcccggtac caatctttta
    25861 tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct tgatcccgaa
    25921 cgccaagtac accaccacca actactttga gttctacctg ccatattact ggaacatcgc
    25981 gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca acatcatgtg
    26041 ggagaacgaa ttccgctacc tctcccaggc gggcgctggc ttgatggaac tggactatct
    26101 gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac gtcgttggtt
    26161 attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac
    26221 caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt ccagtactga
    26281 cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca atgccaccgt
    26341 ttcaaccaag cagttccagg tcttcagcga acagaacacc agtagctact cggcagagcc
    26401 gcagttagac gttaactact accagaatga tgttggtccg tttgatacgc gtatttacgg
    26461 ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc gtgttcacct
    26521 ggaaccgacc atcaatttgc cgctctctaa taactggggc agtatcaata ccgaagcgaa
    26581 gttgctggca acccattatc agcaaactaa tcttgactgg tataactcca gaaacacgac
    26641 caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg gcaaaatggt
    26701 ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg aaccgcgcgc
    26761 gcagtatttg tacgtgccat atcgcgatca gagcgacatc tataactacg actcatctct
    26821 gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc ttgaccgtat
    26881 tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg atgctgccgt
    26941 tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc gcactggcga
    27001 tgacaacata acatgggaga atgacgacaa aacgggctca ctggtgtggg caggcgatac
    27061 ttactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg atacacgtct
    27121 ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag accgtctggt
    27181 acagctgaat taccgttacg ccagcccgga atatattcag gctacgctgc ctaagtacta
    27241 ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc
    27301 aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg cgaacaagca
    27361 agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta
    27421 cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg acaacgcaat
    27481 cggctttaac atcgaacttc gcggcctgag ctccaattac ggtctgggta cgcaagagat
    27541 gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt taccacgtaa
    27601 tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg
    27661 gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat aaagtcgcag
    27721 ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg cagtcggtaa
    27781 aactgaacgc tgctcaggca aggcagcagc ttcctgatga cgcgacgctg cgccaccaaa
    27841 tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa atgggagtga
    27901 aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag aacaacatga
    27961 cgctggatca gatgcgcagc cgtctggcct acgatggtct gaactacaac acctatcgta
    28021 accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca
    28081 tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg
    28141 ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac ccgacctctg
    28201 atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg
    28261 ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg aacggcggcc
    28321 agatgggctg gggccgtatt caggagttgc cggggatctt cgcccaggca ttaagcaccg
    28381 cgaagaaagg cgacattgtt ggtccgattc gttccggcgt tggcttccat attctgaaag
    28441 ttaacgacct tcgcggcgaa agcaaaaata tctcggtgac cgaagttcat gctcgccata
    28501 ttctgctgaa accgtcgccg atcatgactg acgaacaggc ccgcgtgaaa ctggaacaga
    28561 ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa gagttctcgc
    28621 aggatccagg ttctgctaac cagggcggcg atctcggctg ggctacacca gatattttcg
    28681 atccagcctt ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc
    28741 actcttcatt cggctggcat ttaatcgaac tgcttgatac ccgtaatgtc gataaaaccg
    28801 acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc tcggaagaag
    28861 cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg agcaactaat
    28921 ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg gcccggactt
    28981 agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac
    29041 tctccttacc gaccgggcag cgatactcgg tttgccgctc accctccgcc cttattcccc
    29101 caactcccct gcacaaccgc aaactgcggg cacattaacg ctacttcctg tcgcgctacg
    29161 agaatctgtc actgcggggc agttagcggt tgaaaatggg cattacgtgg tggagacgct
    29221 ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct gcgctgatca caggccccgt
    29281 gcataaaggc gtcattaacg acgcaggcat tccgtttacc ggtcataccg agtttttcga
    29341 agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac ttcgcgtggc
    29401 gctggcaacg acgcatttac cgctgcgcga tatcgcagac gctatcaccc ctgcactttt
    29461 gcacgaagtg attgctattt tgcatcacga tttgcggacc aaatttggta ttgccgaacc
    29521 gcgcattctg gtctgtgggc tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga
    29581 agagatagac accattattc cggtgctcga cgaactgcgg gcgcagggga tgaaacttag
    29641 cgggccgctg cctgctgata ccctgtttca gcctaaatat ctcgataacg ccgacgcggt
    29701 gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg
    29761 tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg gcaccgcgct
    29821 tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc ttaatctcgc
    29881 catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca cttagcccgt
    29941 aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga tcgacagcat tgtttctgcc
    30001 attaacccgc agaagggcca ggcgatggtc gaaatcggcc ccggtctggc tgcattgacc
    30061 gaaccggtcg gcgaacgtct ggaccagctg acggttatcg aacttgaccg cgatctggcg
    30121 gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca gcaggatgcg
    30181 atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg tgttttcggc
    30241 aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta tactgatgcc
    30301 attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg
    30361 aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa tgtgatcccg
    30421 gtactggaag taccgccgtc agcctttaca ccaccaccca aagtggattc cgccgtcgtg
    30481 cgcctggttc ctcatgcaac gatgcctcac ccggttaaag atgttcgcgt gttgagccgc
    30541 atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg
    30601 tttagcgtcg aggtgttaac gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc
    30661 tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc tttgcaggag
    30721 agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg
    30781 ctcaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc atacgcaatc
    30841 tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat ggcaatggcc
    30901 gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg
    30961 aagagtacca gtacaccagc ggtgcaatca ttgaaacgcc gctgggcacc atgcagggtc
    31021 actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc gtattccgac
    31081 tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt ggcgacgttc
    31141 atggttgtta cgatgaactg atcgcattgc tgcataaagt agaatttacc cctgggaaag
    31201 ataccctctg gctgacaggc gatctggtcg cgcgcggccc gggttcgctg gatgttctgc
    31261 gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac gatctgcatc
    31321 tgctggcggt atttgccggg atcagccgca ataaaccgaa agatcgcctg acaccgctgc
    31381 tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct ctgctgcaaa
    31441 tcgacgaaga gaaaaagctg gtgatggccc acgcagggat cacgccgcag tgggatctac
    31501 agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac tcctatccct
    31561 tctttctcga cgctatgtac ggcgatatgc caaataactg gtcaccggaa ttgcgggggc
    31621 tgggaagatt gcgttttatc accaacgcct ttacccgtat gcgtttttgc tttccaaacg
    31681 gccaactgga tatgtacagc aaagaatcgc cggaagaagc ccctgcccca ctgaaaccgt
    31741 ggtttgcgat tcctggccct gtcgctgaag aatacagcat cgcctttggt cactgggcat
    31801 cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc tgctgctggg
    31861 gtgggtcatt aacctgcctg cgctgggaag ataaacagta ttttgtccag ccgtcgaacc
    31921 ggcataagga tttgggtgag ggagaggcgg tcgcgtctta aatcccgtcg agcataaaat
    31981 ctaatgctcc tttaatcaac gttcggtgcg cgctggcatc gcaaaatttc gttcccagcg
    32041 cgttaactgg aacagttgca gtctcatgcg tcatgagcac atattctttg ccgtctacca
    32101 gtaaaaggat tgggttaagc cgttctggtg ggcgaatacg gctaaatcgt tcaataggtg
    32161 ttaatggaat aacaatacgg cgattaatca caccaataat gtcgctggta acatcgataa
    32221 caaagggaaa cgcggcgttc ctgctgcgac tgcggtatac cgtaaattgc atcgctagaa
    32281 gctccggtac tcatcagaga aacatccggt ttcatcagcc aattgattta acgcagcgat
    32341 agcttctgcg ttctcttcac gccaacgtgt tgctgcatgt tttttaagtt cagcattgag
    32401 cgcaaccgta agggtggcgc tcatatttaa gcctgcatca cgagcgcgat tgactaactc
    32461 acggtcgacg gtgacggtca cactttgtgt ggtacgtttt gcagtcatga tatcgtgctc
    32521 ctgtacaatt tatacgtcta gtatacacgg atatctgcac gtatcagtga tgccggaatt
    32581 ctaatataca aaattaccgc cgctccagaa tctcaaagca atagctatga gagttctgcg
    32641 catcagcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggga
    32701 aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttt tgcgcttttg
    32761 ccaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt acgtcaccac
    32821 acgccgcgat ggcttcatcc accgacttca cccacgttac gcgatcgtcc gtacccggtt
    32881 gactgctgag gataatattt ttgcgtcctg gcaacggacg accgatggat tcccaggtat
    32941 ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag
    33001 gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct aacgccgcaa
    33061 tcagactgat cattgagatt tcccgataaa aaaaattgtc gccactatac gtaaagcgta
    33121 aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt
    33181 ccacgctctc cccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta
    33241 aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt catccgccat
    33301 gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag
    33361 atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt tatagaccgc
    33421 cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct gaatattaaa
    33481 gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag
    33541 cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctccggct tttcaacgcc
    33601 tgcctgacgc aaacgaatgt agtggtcgac atcgcgggcg cgggcaataa tctgcaaatg
    33661 cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc
    33721 gttaatcagc acttccgctt tcgctgctcc ggcagattcc agtaaatcca tccgcgtggc
    33781 atcgccgtaa aacactttca taccaaattt acgcagcgtt tcgatatggt ccggatcgtg
    33841 atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa
    33901 acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat cggcttcacg
    33961 cgcttcctca gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc
    34021 tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt
    34081 cgccatctgc gccgcgccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac
    34141 cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca gccacagcat
    34201 ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaat
    34261 cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc
    34321 tttaaacggt tcgatatcgc tttccagcgc atgacggtat tccgagcttg ccagcagtac
    34381 gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa
    34441 gccaaacaca aggaataacg ccacggcact aaacacttcc cgcaagccag agcgggcaac
    34501 aaaacgcagc gccggacgcg taacatagcg ccccagcaat accaccagta ccagcgcacc
    34561 cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc
    34621 cagtagcgga atcatcgcca ccagcgggat cgccgcaata tcctggaaca gcagcaccgc
    34681 aaaggcgctg cgacccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc
    34741 aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct gccagcgcag
    34801 cccaagtaac atgcagaaca gccccagcag gccgccgcaa atcaccatct gtaatgcgcc
    34861 accgccgaac accgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat
    34921 aataaacagc atcagcacca caccaatttc agcaaagtgc agaatagatt cggcatcggt
    34981 caccagccgc aacccccacg ggccaataat gcagcctgca atcaggtagc caagtactga
    35041 tcccagaccg aggcgtaccg caatgggtac aatcagcgcc gccgatccga ggtagatcag
    35101 cgcctgaacc agcgtatgac tatccatgat gggcctcctg ccattccagc agacgttgct
    35161 tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg
    35221 caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca
    35281 gcacatcaaa gcccggatgc gcaccaattt caaaatggct tttcccgccg ccggtcgtca
    35341 ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt
    35401 gcgagaaaac tttatcgatc caaagtttaa ggagcggtgg aatgctgtac cactgcatcg
    35461 ggtgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg gcaatatcga
    35521 tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccatcc agcgtccttg
    35581 cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatta
    35641 tgaggatcat agttattagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca
    35701 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaaa
    35761 ttatcccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acattcctcg
    35821 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    35881 tagcatcgtg ttactgatac cgtaattatt gataaatatc ggcagtgccc aggtcgaaac
    35941 aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt
    36001 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    36061 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatta gccccaaaac
    36121 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    36181 gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacatt
    36241 ccctagtgcc gcgtttttgc caacccccaa tcccaacaaa ccaacgattt gcgggccaaa
    36301 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    36361 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    36421 ctcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
    36481 ccctttgcgt aacagccagc ggggagattc tggcagttcg aatcgaccaa tcaaaatcaa
    36541 caaacaaggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
    36601 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg cgataaagct
    36661 gatggaaaat gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg
    36721 ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga
    36781 tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    36841 tttgcgccgt ccgactttat cggaaatata accaaacagc gatgtgccaa cgaacagccc
    36901 ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggcgt
    36961 cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc
    37021 gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct
    37081 ggacggttgc atgggttatt cctgtttttt gcaatgagag tcagtgcggc gttacgtatc
    37141 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    37201 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    37261 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    37321 cagcagctcc agggcctgtt tatcggcatc ggccttcaca acaatgtgcg gatgctcttc
    37381 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    37441 acggtcactc ctttcatgcc atccttgatt agattgatga agcccacttt cttaccgtgg
    37501 cggaggattt tcttgcgcat cagttctggc gcgctgccgt caatggtgaa caggtcacgc
    37561 gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc aaggaacgcc
    37621 ggtagtttct ggtacatacg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    37681 tccgcgagtt tttgcttact gaaatcatcg cttttcatcg ctgaaagcac ggtttttgct
    37741 gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    37801 cacattccgg cggcatcacc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg
    37861 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc acccgcgatc
    37921 agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttc
    37981 gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtgtt ttcattggta
    38041 taaagaaaac cgccgcccat caggccatca gtaggtgatc ccgcaaacag gcaagccgcg
    38101 ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa ctcgatcagt
    38161 tccttcacgc caaccgccac atccgtcggt ttgacgcgtt ttgccatccc caatttttcg
    38221 gcaaggatgg agttcacccc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    38281 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    38341 gtaattaact gcgcacccgc ttcttcggcc tgctccatca accaggcatc aaatttactg
    38401 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccgttgca gtagtccata
    38461 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacg
    38521 ggggcggagt cggcgaaacc tggaataatg tgttccagac tgtgggcata gagacgcccg
    38581 ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcacct
    38641 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    38701 tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccgtaa tgcagccctg
    38761 ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc acggcgtcgc
    38821 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatctttg ttgatggcga
    38881 aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag atccccaccg
    38941 ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    39001 tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct atcgccttac
    39061 aaagctgttc tgccagcgca atgttctctt tgctgccaat gccgcgaccg acgctgacca
    39121 ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg
    39181 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    39241 ctggctgagc cgcatcgaac gtgccactgc tgatggtcag taccgcatac ggcgtggcaa
    39301 tgcgttcttc gccaatcgcc aggccgccgt aaaccatgtg tttcactgtt gctttaccgt
    39361 cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt
    39421 ttgccgccag taatttgccg cgacgggtgt ttggcaacag caccaggccg tctgtgccgt
    39481 gctggcgaat agtgtcagcc atgacactgg cgtaatcttc gatcatccga tcgtccggtt
    39541 tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat
    39601 cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    39661 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    39721 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    39781 gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttctga
    39841 ccaggctgcc tctgcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    39901 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    39961 agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc
    40021 aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat
    40081 attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg aagaaccatc
    40141 gccacagagg atcagatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt
    40201 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccgggcc
    40261 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    40321 ggctgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    40381 gagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac catcagcatt
    40441 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    40501 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    40561 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    40621 atccaacttt caatattgtt aagttcctca ccaatattga aaacatggcg tagcaaaaag
    40681 aaattttcaa tattgtttta tagatcacca atattgaaag tcagatgcta tttttttacc
    40741 cacaaaagaa cataacaaac tgattaataa caaaaattaa cggaaagacg tcctctattc
    40801 acccgaaaca aaaatgtgac actaatcaca gaatacagct tattgaatac ccattatgag
    40861 ttagccatta acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa
    40921 acaaacggga acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga
    40981 aaagagaaaa acgggaatag aaccgaaggt tttctttcca ccgttaataa tcgtcggcat
    41041 actttgttgg cttacagtca gagatctgga tgcggcgaat gtcgttatta atgctgtatt
    41101 cagttacgtc accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt
    41161 cggttggttc tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccgcc
    41221 agaatttagc accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt
    41281 actgttctgg ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga
    41341 accgaactcg acaggagcga aagagttggg gctggcttac agcttgttcc actggggacc
    41401 tctgccgtgg gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg
    41461 caaaatggaa gtgattcgcc ccagctcgac actggtgccg ctggtaggtg aaaaacacgc
    41521 caaagggttg ttcggtacca tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat
    41581 gggtaccagt ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg
    41641 cattccgcat accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc
    41701 catttgcgtc gcctgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta
    41761 cctgagcttc ctgatgctgg gttgggtgtt cattgtcagc ggtgccagct tcatcatgaa
    41821 ctacttcacc gattcggtgg ggatgttgct gatgtatctg ccgcgaatgt tgttctatac
    41881 cgatcccatc gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg
    41941 ggtgatttac gccatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt
    42001 gcgtgaactg tgcttcggca tggtgatggg gctgacagca tccacctgga tcctgtggac
    42061 cgtgctcggt agtaacactc tgctgttgat ggataaaaac atcatcaaca ttccaaatct
    42121 gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag
    42181 caccgccacc atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa
    42241 cgcctgctct tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc
    42301 accgctgctg gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct
    42361 ggcgctcggc ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt
    42421 cttcgtcaac attatggtga cgctctcctt tattaaagac gcgaaacaga actggaaaga
    42481 ttaattaacc cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca
    42541 ggaactgttt gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt
    42601 tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat
    42661 gggtatcgac agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac
    42721 tctcgccgcc gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca
    42781 gttgccgggc ggattcaaca ccttcctgcg cgaaggcaca caagagcaga tcgacaagat
    42841 tatggctttc cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc
    42901 gggctccgac gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct
    42961 taatggtagt aagtgtttta ttaccagcag cgcctacacc ccgtacatcg tggtgatggc
    43021 gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa
    43081 accgggcatc aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga
    43141 aatcaccttt gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg
    43201 ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta
    43261 cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt
    43321 tggcgaggct attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa
    43381 attaaactcc atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat
    43441 cacctctggc gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt
    43501 ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg
    43561 cttctggcgt gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct
    43621 gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac
    43681 gctgacgcgt cttatcaggc ctacgggagc acgaatgtag gccggataag gcgtttacgc
    43741 cgcatccggc agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaccc
    43801 atgccgaaat tcggaccgct ggccggattg cgcgttgtct tctccggtat cgaaatcgcc
    43861 ggaccgtttg ccggacaaat gttcgcagaa tggggcgcgg aagttatttg gatcgagaac
    43921 gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat
    43981 ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta
    44041 atggaaacca ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc
    44101 attaccgatg aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt
    44161 tttggtcagt acggcaccga ggagtacacc aatcttccgg cctataacac catcgcccag
    44221 gccttcagtg gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg
    44281 tataccgccg attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat
    44341 aaagcgcgtg aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg
    44401 cgtatgggcc agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg
    44461 agcaaaggta aagatcccta ctacgccggt tgcggtctgt ataaatgcgc cgacggctac
    44521 atcgtgatgg aactggtggg cataacccaa attgaagagt gctttaaaga tattggcctc
    44581 gcacatctac tcggtacacc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa
    44641 tgcccttacg gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc
    44701 gcagaagtta aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta
    44761 ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg
    44821 atggatggtc gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga
    44881 caaatctggc gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc
    44941 ggctacagcg aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac
    45001 taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca aacgaacaaa
    45061 aatggataga ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga
    45121 tctggcggac gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt
    45181 taaccggtat agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta
    45241 tacgctgggg attcgcaaag gcgacaaggt tgcactacat ctcgacaact gcccgaaatt
    45301 tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg
    45361 cctgttacgc gaggaaagcg cgtggatcct gcaaaatagc caggcgtgcc tgctggtgac
    45421 aagtgcgcaa ttctatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg
    45481 gcacatttgc ctgacagatg tggcacttcc cgctgatgat ggcgtgagtt cgtttactca
    45541 actgaaaaat caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac
    45601 ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca
    45661 ttacaacctg cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga
    45721 cgtctacctg acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc
    45781 ggcgttttct gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg
    45841 gggacaggtg cagaagtacc gcgccaccat taccgaatgt attccgatga tgattcgtac
    45901 gttgatggta cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt
    45961 ttatctcaac ttgtcggagc aggaaaaaga tgcgttttgt gaacgcttcg gcgttcgctt
    46021 gctgacgtct tatgggatga cggaaaccat tgtgggcatt atcggcgatc gccctggcga
    46081 taaacgacgc tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga
    46141 cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc
    46201 agggaaaacc atcttcaaag agtattttct caacccgaaa gccactgcaa aagtgctgga
    46261 agctgatggc tggctgcata ccggcgatac cggataccgc gacgaagaag gcttttttta
    46321 tttcgtcgat cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga
    46381 gctggaaaat atcatcgcca cccatccgaa aattcaggac atcgtggttg tgggtattaa
    46441 agattcgatt cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt
    46501 gagcgaagag gaattttttc gcttctgcga acaaaatatg gcgaaattta aagtgccctc
    46561 ttatctggag atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa
    46621 tctgaaataa atgaaacggc agggaactac cctgccagct aataatcacg ctctaaaaca
    46681 atatgcattt tttgcaggga tgttgtcgtc cctgaaaaag caaaaatgga gaaaaggaat
    46741 gagtgaatca ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgccc
    46801 aaaagcgaat gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt
    46861 ccgtgacgat ccgcaattac gtgtcgccat tattaccggt gccggagaga aattcttttc
    46921 cgcgggctgg gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc
    46981 gggtggtttt gcgggattaa ccgaaatttt caatctcgac aaaccggtta tcgcagctgt
    47041 gaacggctac gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg
    47101 tgccgataac gccagcttcg ccctgccgga agccaaactg ggcatcgttc ctgacagcgg
    47161 cggcgtgctg cgtctgccga agatcctgcc gcctgccatc gtcaatgaaa tggtgatgac
    47221 cggcagacga atgggcgcag aagaggcgct gcgttgggga gtagtcaacc gcgtggttag
    47281 ccaggcggaa ctgatggata acgcccgcga actggcgcag caactggttg acagcgcccc
    47341 gctggcgatc gcagcgctga aagagatcta ccgcaccacc agcgaaatgc cggtagaaga
    47401 agcgtatcgc tatattcgca gcggcgtgtt gaaacactat ccatcagttc tgcattcaga
    47461 agatgccatt gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg
    47521 ttaacaaccg tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg
    47581 tttgtccatc ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc
    47641 ggcccactcg cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat
    47701 attcaggatg gctgcattat gcatggctac tgcgacactg acactatcgt tggggaaaac
    47761 ggccatatcg ggcacggagc gatcctgcat ggttgtgtga ttggtcacga tgcattggtc
    47821 gggatgaaca gcgtgattat ggatggtgcg gtcattggcg aagagagcat tgttgccgcc
    47881 atgagctttg tcaaagcggg ctttcgcggc gagaaacgtc agttgttgat gggtacgcca
    47941 gcccgcgcgg tacgcactgt tagtgacgac gagttacact ggaaacggtt gaataccaaa
    48001 gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac atgaaacaca gccactgagg
    48061 caaatggagg aaaatcgccc ccgtttgcag gggacgacgg atgtggcgcc gaaacggtaa
    48121 tcttgccgga tgcggcgtaa acgccttatc caactctgct ggagagcaac cgtaggccgg
    48181 ataagatgcg ccagcatcgc atccggcgac caggtcgcac ggtatcatcg aaatgacatt
    48241 aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta agttccgtga
    48301 gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc cgactgttat
    48361 tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt cgctgacact
    48421 ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt atttctgtga
    48481 cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata tcgaaatgaa
    48541 tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg gcgattaaca
    48601 agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca caaattaaaa
    48661 gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt gtggatatat
    48721 cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa
    48781 atatcgattc ccgaaaagat ttacttaatg ggcaaaaaaa acggtcctca tcagagaacc
    48841 gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc gccatgacac gctattattt
    48901 gatctgcgcg tgcatttcct gcaccgaaat tactttttcg gtcgcatcgg cattcagcgc
    48961 catcgcggtg gcaaagccgc cgttcagggt ggtgtcgtaa tgcactttat attgcagcgc
    49021 actgcgacgg atcacgcggg agtcttcaat cgcgcgacga cctgaagtgg tgttgatgat
    49081 gtaggtatat tcgccattct tgatgcggtc ctgaatgtgc ggacgacctt catgcacctt
    49141 gtttaccaga cgcggattga tacccgcttc gcccagcaca atcgccgtgc cgtgggtcgc
    49201 atccagctcg aagccctgtt tcagcagttt tgccgccagg tccaccacgc gttctttatc
    49261 gccttcgcgc acggaaagca gcgcacgacc gtgtttcttc atggtggagt tgctgcccag
    49321 ctgcgctttg gcaaacgctt cagcgaaggt gcggcccacg cccatgactt ccccggtaga
    49381 gcgcatttct ggccctaaca gcgggtcaac gcccgggaat ttattgaacg gcagcaccac
    49441 ttctttcacc gagtagtacg gcgggataac ttctttggtt acgccctgct cagccagcga
    49501 tttgccagcc atcacacgcg ccgccacttt tgccagcggt acgccggtgg ctttggagac
    49561 gaacggaacg gtacgcgccg cacgcgggtt aacttcaatc aggtagactt cgttgttttt
    49621 caccgcaaac tgcacgttca tcaggccgcg cacctgcaat tcgaaggcca gtttctgcac
    49681 ctgctggcgc atcacatcct gaatttcctg acttaaggtg tacgctggca gcgaacatgc
    49741 ggagtcaccg gagtgcacgc ccgcctgctc gatgtgctcc atgatgccgc caatcagcac
    49801 catttcgccg tcgcagatgg catccacgtc aacttctact gcgtcatcaa ggaaatgatc
    49861 cagcaacact ggcgcatcgt tagacacgct aaccgccgtc tggaagtagc gacgcaggtc
    49921 agcttcgtca tagacgattt ccatcgcccg accgccgaga acgtaagacg gacgcaccac
    49981 cagcgggtag ccaatctctt ttgccttctc aactgccatt tcaatagcag taacggtggc
    50041 gttcgccggt tgtttcagtt tcagacggtc aaccgcatgc tggaagcgtt cacggtcttc
    50101 cgcacggtcg atagcatccg ggctggtgcc gataaccggt acgccagcgg cttccagtgc
    50161 acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg ataacgcctt tcggcttctc
    50221 aatacgcacg atttccagca catcttccag agttaccggc tcgaagtaga ggcgatcgga
    50281 agtgtcgtag tcggtagaga cggtttccgg gttacagtta accataatgg tttcgtaacc
    50341 gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag tcgaattcga taccctgacc
    50401 gatacggttt ggaccgccgc caagcaccat gattttttca cggtcggtag acggattcgc
    50461 ttcgcactct tcttcataag tggagtacat gtaagcggtg tcggtggcaa actctgccgc
    50521 acaggtatcc acgcgcttgt agaccgggtg caggtcatac tggtcacgca gcttacggat
    50581 ttccgcttcg cgtacgcccg ccagttttgc caagcgcgca tcggcaaagc ctttgcgttt
    50641 cagctggcgc aggaattcag cgttcaggcc agtgatgccc acttccgcca ctttctcttc
    50701 cagacgcacc agttcttcaa tctgtaccag gaaccagcgg tcaatgttag tcaggttgaa
    50761 gacgccgtcc acagacaggc ccgcgcggaa agcatcggcg atgtaccaga tacgctctgc
    50821 gcctgcgtct ttcagttcgc gacggatttt ggttaacgct tccgggtcat ccaggctcac
    50881 tttcgggtcg aatccagtcg caccgacttc caggccgcgc agcgcttttt gcagggattc
    50941 ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat ttcatctgag tggtcagacg
    51001 gtcgttagca ccggcgaatt tttcgaagtt gaagcgagga attttagtaa ccacatagtc
    51061 gatggacggc tcgaaggagg ccggagtacg tccgccagtg atgtcgttca tcagttcgtc
    51121 gagggtgtaa cccaccgcca gtttcgccgc cactttagca atcgggaaac cggtcgcttt
    51181 cgacgccagc gccgaagaac gcgacacgcg cgggttcatt tcgataacaa tcaggcgacc
    51241 gtttttcggg ttcaccgcaa actggacgtt ggaaccaccg gtttcaacgc cgatttcacg
    51301 cagcaccgcc atcgaggcgt tacgcatgat ttgatattct ttgtcggtca gcgtttgggc
    51361 tggcgcgaca gtgatggaat caccagtgtg gatgcccatc gcatcgaagt tttcgataga
    51421 gcagacgatg atgcagttgt cgtttttatc acgcaccact tccatctcgt actctttcca
    51481 gccgatcagc gactcatcaa tcagcaactc tttggtcgga gagagatcca gaccgcgggc
    51541 gcaaatttct tcaaactctt cgcggttata agcgataccg ccgccgctac cgcccatggt
    51601 aaaggatggg cgaataatgc acgggaagcc cacgtcagcg gcaaccgcca gcgcttcttc
    51661 catcgtgtgt gcgataccgg aacgcgcggt ttccagacca attttcttca tcgctacgtc
    51721 gaaacgacgg cggtcttctg ctttatcaat cgcatcggca gtggcaccaa tcatggtgac
    51781 gccgaactct tccaacacgc cctgacgttc cagctccagc gcgcagttca gcgccgtctg
    51841 accgcccatg gttggcagca ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac
    51901 ttcccagtga atcggctcga tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt
    51961 cgccgggttg gagttcacca gaatgacgcg gtaaccctct tcgcgcaggg ctttacatgc
    52021 ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata acaatcgggc ccgcacccag
    52081 aatcaggata ctttttatat ctgtacgttt tggcatggct cttttactcc tgattactta
    52141 gcggttttac ggtactgctc aattaactcg ataaagtggt cgaacaacgg cgcggcgtcg
    52201 tgtggaccag ggctggcttc agggtgaccc tggaagctga acgccggttt atcggtgcga
    52261 tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag gtttgcaggt
    52321 aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc
    52381 tcaacatctt taaccggatg gttgccgccg tggtgaccaa atttcatttt gacagtcttc
    52441 gcaccgctcg ccagcgccag cagctgatga ccgaggcaga tgccgaatac cggaatagcg
    52501 gtttcgagga atttctggat ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga
    52561 ccgttggaga ggaagatgcc gtctggattc attttcagca catcttccgc agaagtttgc
    52621 gccggaacga tggtcaggcg acagcctcta tccaccagca tccgcaggat gttgcgcttg
    52681 gcaccaaaat cataagccac gacgtggaac ggcagctcgt cttctttttt cgcttctggc
    52741 aggccgccgg tcaacgtcca gctcccttgt gtccagctat aggcttctgc ggtggtcact
    52801 tcttttgcca gatccatgcc gttcagacct gggaacgcgc gggctttttc taacgccagc
    52861 gccgcatccg ggttatcgcc tgcgataatg cagccattct gtgcgccttt ctcgcgcagt
    52921 aaacgtgtca gcttacgggt atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg
    52981 taagaagata ggtcttcggt attacggaag ttgctggcaa tcagcggcag atcgcgaatc
    53041 accagacctt gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt gccgacattg
    53101 ccaatatggg gataagtaag agtaacgatt tgacgagaat aggaaggatc agtgaggatt
    53161 tcttgataac cggtcattga agtattgaaa acgacttccc caaccgccga acctgttgcc
    53221 cctatggccc gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc tgacttaatc
    53281 aaaacaccct ccagagaata ttcactcact ttatttgcat attaattcac aatgatgacg
    53341 taaatcaatg caaatctgct taccgacgaa tttctggcaa acggcggcat tctggagata
    53401 tgggcgctaa aagtcaactt aatggcggga ttttttatat tattttgtgg ctcatgcatg
    53461 ttttacaggc attaagcgac aaaaagatca tctaacctgt tctggaaaac gcttgcgcag
    53521 caagaagatt gcatgaagta aaaacgagta gaaaaaagtg gaccaaatgg tcaaaattta
    53581 cattaaaaca acaaaaatag aagacaaaa
//
LOCUS       DAZGCQ010000022        52656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000022 DAZGCQ010000000
VERSION     DAZGCQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.022"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..593
                     /gene="yraH"
                     /locus_tag="ACHK07_001395"
     CDS             9..593
                     /gene="yraH"
                     /locus_tag="ACHK07_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101751.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /gene="yraI"
                     /locus_tag="ACHK07_001396"
     CDS             673..1368
                     /gene="yraI"
                     /locus_tag="ACHK07_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8101752.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1397..3913
                     /gene="yraJ"
                     /locus_tag="ACHK07_001397"
     CDS             1397..3913
                     /gene="yraJ"
                     /locus_tag="ACHK07_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8101753.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYSYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3924..5015
                     /gene="yraK"
                     /locus_tag="ACHK07_001398"
     CDS             3924..5015
                     /gene="yraK"
                     /locus_tag="ACHK07_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101754.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5058..5918)
                     /gene="rsmI"
                     /locus_tag="ACHK07_001399"
     CDS             complement(5058..5918)
                     /gene="rsmI"
                     /locus_tag="ACHK07_001399"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO8101755.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHK07_001400"
     CDS             5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHK07_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO8101756.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7977..8372
                     /gene="yraN"
                     /locus_tag="ACHK07_001401"
     CDS             7977..8372
                     /gene="yraN"
                     /locus_tag="ACHK07_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO8101757.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8392..8982
                     /gene="diaA"
                     /locus_tag="ACHK07_001402"
     CDS             8392..8982
                     /gene="diaA"
                     /locus_tag="ACHK07_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO8101758.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8992..9567
                     /gene="dolP"
                     /locus_tag="ACHK07_001403"
     CDS             8992..9567
                     /gene="dolP"
                     /locus_tag="ACHK07_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8101759.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9681..10721)
                     /gene="yraQ"
                     /locus_tag="ACHK07_001404"
     CDS             complement(9681..10721)
                     /gene="yraQ"
                     /locus_tag="ACHK07_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8101760.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10794..11429)
                     /gene="yraR"
                     /locus_tag="ACHK07_001405"
     CDS             complement(10794..11429)
                     /gene="yraR"
                     /locus_tag="ACHK07_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO8101761.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHK07_001406"
     CDS             11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHK07_001406"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO8101762.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12055..12498)
                     /gene="yhbP"
                     /locus_tag="ACHK07_001407"
     CDS             complement(12055..12498)
                     /gene="yhbP"
                     /locus_tag="ACHK07_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO8101763.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHK07_001408"
     CDS             12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHK07_001408"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO8101764.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12838..13341)
                     /gene="yhbS"
                     /locus_tag="ACHK07_001409"
     CDS             complement(12838..13341)
                     /gene="yhbS"
                     /locus_tag="ACHK07_001409"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8101765.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13335..13859)
                     /gene="ubiT"
                     /locus_tag="ACHK07_001410"
     CDS             complement(13335..13859)
                     /gene="ubiT"
                     /locus_tag="ACHK07_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO8101766.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14068..15063
                     /gene="ubiU"
                     /locus_tag="ACHK07_001411"
     CDS             14068..15063
                     /gene="ubiU"
                     /locus_tag="ACHK07_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO8101767.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15072..15950
                     /gene="ubiV"
                     /locus_tag="ACHK07_001412"
     CDS             15072..15950
                     /gene="ubiV"
                     /locus_tag="ACHK07_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO8101768.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16031..17038
                     /gene="yhbW"
                     /locus_tag="ACHK07_001413"
     CDS             16031..17038
                     /gene="yhbW"
                     /locus_tag="ACHK07_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO8101769.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17156..18400)
                     /gene="mtr"
                     /locus_tag="ACHK07_001414"
     CDS             complement(17156..18400)
                     /gene="mtr"
                     /locus_tag="ACHK07_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO8101770.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18554..20443)
                     /gene="deaD"
                     /locus_tag="ACHK07_001415"
     CDS             complement(18554..20443)
                     /gene="deaD"
                     /locus_tag="ACHK07_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO8101771.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20436..20516)
                     /gene="yrbN"
                     /locus_tag="ACHK07_001416"
     CDS             complement(20436..20516)
                     /gene="yrbN"
                     /locus_tag="ACHK07_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO8101772.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20623..21507)
                     /gene="nlpI"
                     /locus_tag="ACHK07_001417"
     CDS             complement(20623..21507)
                     /gene="nlpI"
                     /locus_tag="ACHK07_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO8101773.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21616..23751)
                     /gene="pnp"
                     /locus_tag="ACHK07_001418"
     CDS             complement(21616..23751)
                     /gene="pnp"
                     /locus_tag="ACHK07_001418"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO8101774.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23998..24267)
                     /gene="rpsO"
                     /locus_tag="ACHK07_001419"
     CDS             complement(23998..24267)
                     /gene="rpsO"
                     /locus_tag="ACHK07_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO8101775.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24416..25360)
                     /gene="truB"
                     /locus_tag="ACHK07_001420"
     CDS             complement(24416..25360)
                     /gene="truB"
                     /locus_tag="ACHK07_001420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO8101776.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25360..25761)
                     /gene="rbfA"
                     /locus_tag="ACHK07_001421"
     CDS             complement(25360..25761)
                     /gene="rbfA"
                     /locus_tag="ACHK07_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO8101777.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25926..28598)
                     /gene="infB"
                     /locus_tag="ACHK07_001422"
     CDS             complement(25926..28598)
                     /gene="infB"
                     /locus_tag="ACHK07_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO8101778.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28623..30110)
                     /gene="nusA"
                     /locus_tag="ACHK07_001423"
     CDS             complement(28623..30110)
                     /gene="nusA"
                     /locus_tag="ACHK07_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO8101779.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30138..30590)
                     /gene="rimP"
                     /locus_tag="ACHK07_001424"
     CDS             complement(30138..30590)
                     /gene="rimP"
                     /locus_tag="ACHK07_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO8101780.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30797..30873)
                     /locus_tag="ACHK07_001425"
     tRNA            complement(30797..30873)
                     /locus_tag="ACHK07_001425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30837..30839),aa:Met,seq:cat)
     gene            31221..32564
                     /gene="argG"
                     /locus_tag="ACHK07_001426"
     CDS             31221..32564
                     /gene="argG"
                     /locus_tag="ACHK07_001426"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO8101781.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(32572..34197)
                     /gene="yhbX"
                     /locus_tag="ACHK07_001427"
     CDS             complement(32572..34197)
                     /gene="yhbX"
                     /locus_tag="ACHK07_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8101782.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34656..34742)
                     /locus_tag="ACHK07_001428"
     tRNA            complement(34656..34742)
                     /locus_tag="ACHK07_001428"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34706..34708),aa:Leu,seq:gag)
     gene            complement(34757..35089)
                     /gene="secG"
                     /locus_tag="ACHK07_001429"
     CDS             complement(34757..35089)
                     /gene="secG"
                     /locus_tag="ACHK07_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO8101783.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35317..36654)
                     /gene="glmM"
                     /locus_tag="ACHK07_001430"
     CDS             complement(35317..36654)
                     /gene="glmM"
                     /locus_tag="ACHK07_001430"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO8101784.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36647..37495)
                     /gene="folP"
                     /locus_tag="ACHK07_001431"
     CDS             complement(36647..37495)
                     /gene="folP"
                     /locus_tag="ACHK07_001431"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO8101785.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37585..39519)
                     /gene="ftsH"
                     /locus_tag="ACHK07_001432"
     CDS             complement(37585..39519)
                     /gene="ftsH"
                     /locus_tag="ACHK07_001432"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO8101786.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39619..40248)
                     /gene="rlmE"
                     /locus_tag="ACHK07_001433"
     CDS             complement(39619..40248)
                     /gene="rlmE"
                     /locus_tag="ACHK07_001433"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO8101787.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40374..40667
                     /gene="yhbY"
                     /locus_tag="ACHK07_001434"
     CDS             40374..40667
                     /gene="yhbY"
                     /locus_tag="ACHK07_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO8101788.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40823..41299)
                     /gene="greA"
                     /locus_tag="ACHK07_001435"
     CDS             complement(40823..41299)
                     /gene="greA"
                     /locus_tag="ACHK07_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO8101789.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41547..42980
                     /gene="dacB"
                     /locus_tag="ACHK07_001436"
     CDS             41547..42980
                     /gene="dacB"
                     /locus_tag="ACHK07_001436"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8101790.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHTRFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43020..44192)
                     /gene="cgtA"
                     /locus_tag="ACHK07_001437"
     CDS             complement(43020..44192)
                     /gene="cgtA"
                     /locus_tag="ACHK07_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO8101791.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44208..45173)
                     /gene="yhbE"
                     /locus_tag="ACHK07_001438"
     CDS             complement(44208..45173)
                     /gene="yhbE"
                     /locus_tag="ACHK07_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8101792.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45300..45557)
                     /gene="rpmA"
                     /locus_tag="ACHK07_001439"
     CDS             complement(45300..45557)
                     /gene="rpmA"
                     /locus_tag="ACHK07_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO8101793.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45578..45889)
                     /gene="rplU"
                     /locus_tag="ACHK07_001440"
     CDS             complement(45578..45889)
                     /gene="rplU"
                     /locus_tag="ACHK07_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO8101794.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46148..47119
                     /gene="ispB"
                     /locus_tag="ACHK07_001441"
     CDS             46148..47119
                     /gene="ispB"
                     /locus_tag="ACHK07_001441"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO8101795.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPKEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47347..47625
                     /gene="sfsB"
                     /locus_tag="ACHK07_001442"
     CDS             47347..47625
                     /gene="sfsB"
                     /locus_tag="ACHK07_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO8101796.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47673..48932)
                     /gene="murA"
                     /locus_tag="ACHK07_001443"
     CDS             complement(47673..48932)
                     /gene="murA"
                     /locus_tag="ACHK07_001443"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO8101797.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48987..49241)
                     /gene="ibaG"
                     /locus_tag="ACHK07_001444"
     CDS             complement(48987..49241)
                     /gene="ibaG"
                     /locus_tag="ACHK07_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO8101798.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49401..49694)
                     /gene="mlaB"
                     /locus_tag="ACHK07_001445"
     CDS             complement(49401..49694)
                     /gene="mlaB"
                     /locus_tag="ACHK07_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO8101799.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49694..50329)
                     /gene="mlaC"
                     /locus_tag="ACHK07_001446"
     CDS             complement(49694..50329)
                     /gene="mlaC"
                     /locus_tag="ACHK07_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO8101800.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50348..50899)
                     /gene="mlaD"
                     /locus_tag="ACHK07_001447"
     CDS             complement(50348..50899)
                     /gene="mlaD"
                     /locus_tag="ACHK07_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO8101801.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50904..51686)
                     /gene="mlaE"
                     /locus_tag="ACHK07_001448"
     CDS             complement(50904..51686)
                     /gene="mlaE"
                     /locus_tag="ACHK07_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO8101802.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51694..52503)
                     /gene="mlaF"
                     /locus_tag="ACHK07_001449"
     CDS             complement(51694..52503)
                     /gene="mlaF"
                     /locus_tag="ACHK07_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO8101803.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
BASE COUNT        13053 a        14328 c        12792 g        12483 t
ORIGIN      
        1 ggttataaat gaataaagtt acaaaaacag ctattgcggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgtaggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc
      301 tggaaacgcg ggataatggt accgtcccta acttccctaa cttggcaaaa gtgggctctt
      361 ttgctggcac agcagcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc
      421 ttatgccact aaatgctatg ggcaatgata acacagttta tcagatccct gcggactccg
      481 ctggaattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatccgaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctttca gataacgccc ttttccttcc tctttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcggtgataa taaccttgtt gtgtagcttc
      721 tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac gccttattta
      841 ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca
      901 ccgctatttc gtcttgaagc tggcgatgat tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgagccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cttgaagggg ttggtcaacg atgcgtggaa atcactggaa
     1141 tttaaacgta gtgaccattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggactgg cagtcgataa agccgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tccagcatct ggcggaaaga acgtgaaatg ggctgcgatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 cacaggtcat cagggaatgc cacaacgaca ccaccaggga cataaacgca caccgaaaca
     1441 gttggcgctc attatcaaac gctgtttgcc gatggtgctc actggcagcg gcatgctttg
     1501 cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa ccacaaaaag
     1561 tggtatgcaa acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac taccaggaac
     1621 ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa ttacatttac
     1681 cgccaatgca gagcaacttc tgcagccaca gtttacggta gaacaactac gtgagctggg
     1741 tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg tgatcaactc
     1801 gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc agcgacttaa
     1861 tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct ccccttctcg
     1921 ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt ctgataaccg
     1981 ttaccgccag ggcaatcgta gccaacgaca gtacctgaat atgcaaaatg gtgctaattt
     2041 tggcccctgg cgattacgta actattctac gtggacacgc aacgatcagg cgtcaagctg
     2101 gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaagtctc agttgcttct
     2161 gggagaaagc gccaccagcg gcagtatttt ttccagctac acctttactg gcgtgcaact
     2221 cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa cggtacgcgg
     2281 tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga tctatcaaag
     2341 caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca acagcggcga
     2401 tttagaagtc acgattgaag aaagtgacgg tacacaacgt cgctttatcc agccttattc
     2461 ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg ctggacgcta
     2521 tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca cggcaatata
     2581 tggtttgaat aatactttta cgctgtatgg cggcctgctc ggttctgaag attattatgc
     2641 gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga tggatatcaa
     2701 cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat ggcgtacgca
     2761 gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact atagctatac
     2821 caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact ataacagtcg
     2881 ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg taagtctgta
     2941 tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga atatctctgt
     3001 tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat acagccgcta
     3061 cactgatcaa aataatgacc gcgcactctc tttgaatctc agtattccgt tagaacgctg
     3121 gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc caacccaaca
     3181 tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata gcctggaaca
     3241 aagtctggat gacgataaca accataacag tagcctgaac gccagttacc gttcacctta
     3301 tggaaccttc agtgccggat acagttacgg taatgacagc agccaataca attacggcgt
     3361 taccggcggc gtggttatcc atcctcatgg cgtgacgctc tcgcaatatc tgggcaacgc
     3421 ttttgcgctt atcgatgcta acggggcatc tggcgtgagg atacaaaact atccggggat
     3481 tgctaccgat ccctttggct atgcagtggt tccttatctc acaacttatc aggaaaaccg
     3541 tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa caacacagtt
     3601 tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata tcggttatcg
     3661 cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg ctcttgccag
     3721 caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat atctctctgg
     3781 gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag atcaacaatg
     3841 tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac aaggcacagc
     3901 gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga cttttgttga
     3961 tatccacatc ctgcgcttat gcctcctcag gagggtgtgg agccgacagc actagcggtg
     4021 cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat aacgtgacag
     4081 gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct
     4141 ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac
     4201 atcagacagg atattacaaa ctcaatgaca gcctggatat taaaaccaca ttacaggcaa
     4261 acgacattcc aggactcaca accgaccagg ttgtctctgt taacacccga ttcacacaga
     4321 taaaaagcag caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt
     4381 ctcgttatgg acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc
     4441 catttctcgg ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg
     4501 tcgatggaat gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgatac
     4561 agggaaatct caccgcccca cagtcgtgca aaattaatca gggcgatgtt attaaggtta
     4621 attttggatt catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta
     4681 ctccagtaga ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt
     4741 tgcaaatgcg catcgacggt acaactgggg tagtagacca gtacaacctg gtcgccagac
     4801 gaagaagttc agacaatgcg cccgatgtcg gtattcgtat tgaaaatctc ggtggcggag
     4861 ttgcaaatat tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg
     4921 tcaatatgcg cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc
     4981 agggcacagc caccattacc gtcatcgtgc ggtaaaagaa gacgttgcgt tttgattaac
     5041 gcaacgcttc ggcactctta cccctgctgc tccagcgcat acttatatag cgcatttttc
     5101 ttcacgccgt gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt
     5161 agcagcgcca gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc tttatgacct
     5221 tcgacaatca gcaccatttc gcctttgcga cggttttcat cttcctttac ccacgccagc
     5281 agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc acgcgccaga
     5341 accacgtagc gggattcgcc taataccgca acgatatctt ccaggctatc taacagacgg
     5401 tgggtagatt cataaaaaat cagcgtgcgc ggctccgctt caatggcttt tagcgcatca
     5461 cggcggcctt ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa
     5521 cccgcggcgc ttaacgcagt gatagcagca cacggtccgg gtagcggcac cacacgaatc
     5581 cccgcttcac ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg
     5641 gcatcggaaa ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct
     5701 ttttgttgtt cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc
     5761 agcaataaac cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc
     5821 tctaacgcac gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc
     5881 tggccctgag aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt
     5941 taatattgag cattgcgtaa aaaaaatatc actggataca ttatggtacc ctcaacattt
     6001 tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgtcggt
     6061 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     6121 tctgcctttt atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa
     6181 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt
     6241 aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta
     6301 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaattaca
     6361 cccgccgatt tagaacaaaa ccagcaagcc cgttactggc aggcaaaaat cgatgccagc
     6421 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgcttggc
     6481 gcgaaagaaa aacagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag
     6541 gaacaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     6601 ctgcagcgcg tctggtttga taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc
     6661 gactggcaga aacgttatcc gaacaacccg ggcgcgaaaa tgctgccaac gcagttggtt
     6721 aacgtaaaag cgtttaaacc agcctcgacc aacaaaatcg ccctgctgtt gccactgaat
     6781 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     6841 ggcactcagc cagtggcagc tcaggtagct gccgcacctg ccgcagacgt agcagaacaa
     6901 ccccaaccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg
     6961 accggtgaac agcctgcagc ccagccggtg cctgtaagcg ccccggcgac aagcaccgca
     7021 gcggtaagcg cacccgcaaa tccatccgca gagctgaaaa tctacgatac ctcatcacaa
     7081 ccacttagcc agatcttaag ccaggttcag caggatggcg cgagtattgt ggtcggtccg
     7141 ttgctgaaaa ataacgttga agagttgctg aagagcaaca ctccgctgaa cgtactggca
     7201 ctgaaccagc cggagaatat cgaaaatcgc gtcaatattt gttacttcgc gctttcaccg
     7261 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     7321 gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaagagtgg
     7381 cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     7441 gcgggtgtta acggcggttc tggtatcgct ttaacgggta ccccgattac tcccagagcg
     7501 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     7561 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     7621 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     7681 tacgccagct cccgcagtgc gcaagggacc gctggcccgg atttccgact ggagatggaa
     7741 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctaccgtt aatgcagcag
     7801 gcactcagcg cggtgaataa cgattattca ctggctcgca tgtatgcgat gggcgtcgat
     7861 gcctggtcgc tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac
     7921 ggtaataccg gaagcctgac ggctaacccg gattgcgtga ttaacaggaa gttatcatgg
     7981 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     8041 cgtgggaagt acaagcgcgt cgctggctgg aaggcaaagg actgcgattt gtcgccgcta
     8101 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     8161 ttgtcgaggt gcgttaccgc cgttctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     8221 gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga
     8281 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     8341 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     8401 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     8461 ccggatgcca tctcccgtgc ggccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     8521 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     8581 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     8641 gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     8701 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc
     8761 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgaccattgt ggcattgacc
     8821 ggctatgacg gcggtgaact tgcaggtttg ttagggccac aggatgtgga gatccgcatc
     8881 ccttcgcatc gcagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     8941 gatctgatcg ataacacgct tttccctcac caggatgatt aaggagaata catgaaggca
     9001 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     9061 gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     9121 caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag
     9181 attaagaaag aagcgcgcat taatgtaacg gcctatcagg gcaaagtgct gctggttggg
     9241 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     9301 gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcgtct
     9361 aatgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa
     9421 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     9481 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg tgagcggcgt gaagcgggta
     9541 actacggcgt ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc
     9601 gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat
     9661 ccgacatgtt ttccctcaaa tcagaataac agcgccagcc cgccgacaat cacgcctgac
     9721 actgccacca tcgcccccgt cagccataag gctttcgccg ggaacgcttt gcgcagcata
     9781 atcagtgacg gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcagtt
     9841 cccatacctg ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca
     9901 aacaagcatc ctgctaccgc catcgccacc acccacatca ggctgttatc gacagcacca
     9961 tcggcatggg ggaataacca gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa
    10021 accgggatcg tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaatccg
    10081 ccctgtgctt ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc
    10141 catttttgca ccagcgtcgc aatcagcaac accatcacca gcccggccac cagacgaatc
    10201 gccgcaaaac cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacacc
    10261 ggattgccca tccagaatgc cagcgcaccg cccatcgaca cctgttggcg acgcattccc
    10321 gccgcgaccg gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc
    10381 agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca acaaccagtc acgcgggatc
    10441 agcacctgaa tcaacgaacc gaggatcacc cccagcactg ccgctttcca taccgcgagg
    10501 aaatagatca tcgcgtaatc caacgccgct tgccacggat tagcatccgc ctgcgcaagg
    10561 atagatttac cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc
    10621 catttcacat accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac
    10681 tgaatgggcg ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag
    10741 ttatgataat tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca
    10801 gctcttttgc gtaattctga agagcttaaa atagtcacgc cttcatgctc aggccgcatc
    10861 gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggt
    10921 aacaaacgga acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc
    10981 agtaacatcg acgggcgagc aatggtcaat ttcggccagt tctgggcaat taatgcttct
    11041 tccatctccc ctttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca
    11101 ctgaccacca gcatatgctg cgcgcccagc cgccgcccgg ttaatgcggt atccactacc
    11161 agcgtgtaat cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg
    11221 agacaacaaa acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc
    11281 ggatcatggg gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct
    11341 attgcgttaa ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc
    11401 gttgcgcctg taatcagtac ctgactcatc ttcgctcctt tacagaattg tctaccttgc
    11461 gctccacggc tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtggga
    11521 aattcgcctc atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta
    11581 atcactgatg aatttgaaga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc
    11641 ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga
    11701 gaagccagcg tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc
    11761 ctgttgctac cgggcggtca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc
    11821 tttacccgtg attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag
    11881 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ccgcagttaa accgatcatt
    11941 attgatgtta aaaatgcggg cgcggaattt tacgatcagg aagtcgtggt tgataaagat
    12001 cagctggtta ccagccggac gccggacgat ctgccagcgt ttaaccgcga ggcgttacgc
    12061 ctgctcggtg cctgagccgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc
    12121 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
    12181 cggaaagcga cgattgtacg ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg
    12241 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
    12301 cgttccggca accgcagcct gcggcccact catctgcgca tgacgcgttt tttcttccgt
    12361 caaaatgtag aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccagag
    12421 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat
    12481 ggtgatgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
    12541 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
    12601 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
    12661 aagcactgcg cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt
    12721 cgctggcgtt acgggcggaa tatcgcgtta aacaactgac caaacggcaa aaagagcgtc
    12781 tggtagcgga aggcgcaggg tttgcagagc tgttaagcag tctgcaaacc ccggagatta
    12841 aaagcgattg aaatgctcgt gatactcaac cagaccagtt acgccattca acgcgtcatc
    12901 cgctaaacga tgtacctgga aggcgctttc agtgcctggc cagcggcaac gcagatcgtg
    12961 atgtgccgcc agttcaaagc caaaacggct gtacagcgcc ggatcgccca gcgtcaccac
    13021 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
    13081 cccctgtcca cggtattttt catctaccgc caacggtgcc atgccgaccc attgcaggtc
    13141 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
    13201 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
    13261 ttccgcatca ctttcgaatg agcgacgcag cagggcatca atacccggcg catcaatggg
    13321 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
    13381 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
    13441 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
    13501 ccttcaatca ccagccgacg ctggaagaag agcgtatccg gatcctgttt acgcgccgca
    13561 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg
    13621 accaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
    13681 atacttaacc agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
    13741 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
    13801 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
    13861 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa taacatagcg
    13921 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
    13981 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
    14041 ttgatagcgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
    14101 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
    14161 accaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
    14221 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg
    14281 gacggttacg cccgttggca gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg
    14341 ctgatcctcg ccgacctcgc catgctggag tacgccgccg agcgttaccc gcatattgag
    14401 cgtcatgtgt cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
    14461 cattttgacg ttgcccgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacag
    14521 ctggcacggg tcacacctgt cccgctggaa gtgtttgctt tcggcagcct gtgcattatg
    14581 tcggaaggtc gttgctatct gtcgtcgtat ctgacgggtg agtcgcccaa caccgtgggc
    14641 gcgtgttctc cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc
    14701 ctgaacgaag tgctgatcga tcgttatcag gacggcgaaa acgcaggtta tccgacgttg
    14761 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
    14821 ctcaataccc tggaactgct accggagtta atggcgtcga atatcgcttc ggtgaaaatt
    14881 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
    14941 atcgaccgtt gtaaggccga tccgcaaaac ttcgtaccgc aaagcgcgtg gatggagacg
    15001 ctcgggtcga tgtccgaagg cacgcaaacc acccttggcg cgtatcaccg taaatggcag
    15061 tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
    15121 tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggcgaag
    15181 cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
    15241 tcgccggaag tggtaagcag atagtgctct ccacgctggc gctggtgcag gcatcatctg
    15301 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
    15361 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccgga cacgcgctga
    15421 actgctataa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
    15481 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagctgg
    15541 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact
    15601 ccgctcgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
    15661 gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac
    15721 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctggcatcca
    15781 tgcagggcct ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc
    15841 tcgacgcctt ccgcgctaat gaaaatggcg ctgcaccact gccgttgacg gcgaacagcg
    15901 actgtaacgg ctactggcgg cggctggcag gactggagct gcaagcctaa gtaaatagct
    15961 cactttgtta acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa
    16021 gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc
    16081 cgaaggttct tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga
    16141 aaagcgcggc tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag
    16201 tgctgccacg tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg
    16261 gtctggcggc gtgatgttgc ctaaccactc accgttggtc attgcagaac agttcggcac
    16321 gctcaataca ctctatccgg ggcgaatcga tttggggctg ggtcgtgctc cgggtagtga
    16381 ccagcgaacc atgatggcgc tacgtcgtca tatgagcggc gatattgata atttcccccg
    16441 ggatgtcgcg gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc
    16501 ggtaccaggc tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc
    16561 gcaactggcg gcgcagcttg gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat
    16621 gctgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcagcac ggctggaaaa
    16681 accatacgcg atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt
    16741 tctgtttacc tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccgggcaact
    16801 gccgccgccg attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca
    16861 gcaggcgctg agtatgtcgt tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc
    16921 gatcctgcgc gaaaccgacg ccgatgagat tatggtcaac gggcagattt tcgaccacca
    16981 ggcgcggctg cattcgtttg agctggcgat ggatgttaag gaagagttgt tgggatagtg
    17041 tgtcttaacg cgggaagcct tatccgagct ggcaacgctg tcctacatag acctgataag
    17101 cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact
    17161 gatacaccgg cagtaaatta aagctcgata aaatatgcac cagtgcgttg ccgacgccaa
    17221 acaccagaat cagcgcaatc atcggcttgc caccccagac gcggaatttc gggctgccaa
    17281 agcgtttacg cgatgcgcgg gccagcagtg ccggaacaat tgccgcccag atggtagccg
    17341 ctaaaccagc ataaccaatg gcgtacagga atccgttcgg gaacagcagg ccccccacga
    17401 caggcggggc aaaggtcagc aatgctgttt tcaagcggcc cacagctgag tcatcgaaac
    17461 caaacagatc tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta
    17521 ccgcaaagtt tgagaacacg accagcagca gatccagact acggctgttc agtacgccgc
    17581 ttaacgcctg taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg
    17641 gacgcgggat gttacccatc gtcgccagca accagatggt atacagcgcc agcgccatca
    17701 gcgtaccgta caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca
    17761 ggcttggcac gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcaaca
    17821 gatacggtgc ataagacgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat
    17881 gccccagcag gctaccaaag gtgaggaaga aggtaatgac tttcgccccc agcacaatcg
    17941 ctgtcatgcg actgacggct ttagtgctca accacaccac aaacgctacc aacaatgcaa
    18001 aaccaaaacc cgccgcccgt gccgggacgt ttagtgacat ctctgcgaag gtgtgatgca
    18061 gaatcgaacc actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg
    18121 aaatgccgtt gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac
    18181 tcgaaccgat tctgtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac
    18241 agaaccaggt aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca
    18301 ctggcagaga aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa
    18361 gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat gagggcttct ctccagtgaa
    18421 aaatagtgcg actgcgttgt tatgtattgc actgtaccag tacacgagta caaaagacag
    18481 aaaaaaagcc ccgatggttc aaatcggggc tgtatatatt attttacaga ctgtgttcgc
    18541 tgttcagcga tgattacgca tcaccaccaa aacgacgacg accggtagag tcatcacgac
    18601 gcggagcgcg gccttcacga cgttcgccgc taaaacgacg accatcacca cgaccacctt
    18661 cacggcgttc accgctgaag ttacggccgc cttcacgacg ctcgccactg aaaccacgac
    18721 caccgccacg acgctcaccg ccagtatgcg gctgtgcatc gcccagtaat tgcatattca
    18781 tcggcttgtt cagaatgcga gtacgtgtaa agtgttgcag cacttcaccc ggcatacctt
    18841 tcggcagttc gatggtggag tgagaagcaa acagcttaat gttaccgatg taacggctgc
    18901 tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc
    18961 ggcccacttc aatacgatac aactgcatgt caccaacatc acgacgttca cgacgcggac
    19021 gatcttcacg gtcgccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac
    19081 ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg cggaacaatc agagtacgtt
    19141 caccctgtgc cattttcagc agtgcggcgg ccagagtttc gagatccagc tcttcacctt
    19201 ctgcagtcgg ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca
    19261 gctgctgctg tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt
    19321 tcggcagttc tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac
    19381 gacgctcgcg gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac
    19441 gaccgatacg gtgaacgtaa gactcagaat ccatcgggat atcgtagtta accaccaggc
    19501 tgatacgctc aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac
    19561 gaccatcttt caggcgttcc agtgtctgct cacgcagcgc ctggttcatg tcaccgttca
    19621 gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcatttt
    19681 tggtacgaac gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg
    19741 cttcgttttt acgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag
    19801 tcacgctgga ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac
    19861 gaatcgcttc cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg
    19921 ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca
    19981 gaaccagacc gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac
    20041 ggcccggagt accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat
    20101 aacgctggcc gccgtacagg gcaaccacat ttacgccgcg catgtgttta gagaaatccg
    20161 tcattgcttc agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg
    20221 gtgctttcag ctcaggatca agattctgca gcagaggtaa agagaatgct gcagtttttc
    20281 cgctccccgt ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact
    20341 ccgcctgaat tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag
    20401 ccttcaggcc cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca
    20461 aaattaatgg cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa
    20521 aagtgtgaac cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg
    20581 tgatggcaat caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc
    20641 tgccaggtca tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc
    20701 aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa
    20761 atcccccaga cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc
    20821 gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat
    20881 gttgcccagg tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa
    20941 gtgctgtttc aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag
    21001 ccacagacta cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg
    21061 cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt
    21121 gtaagttgga tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc
    21181 tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc
    21241 cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac
    21301 tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat
    21361 ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag
    21421 gacttcactt ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac
    21481 gaaacaccag cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg
    21541 tcctgttccc ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga
    21601 gggcaaatgg caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg
    21661 ttgagactgc tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc
    21721 cagaactttc accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg
    21781 tttgtcagcg atttgagaga tgtgtaccag accttcttta ccgccgccga tggcaacaaa
    21841 tgcgccgaag tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc
    21901 tgcagtgatc tcttcgatac gacgaatagc gtgtttcgct ttctcgccgt cggtcgctgc
    21961 gatcttcaca gtaccgtcat cttcgatttc gatggtggtg ccggtttctt cggtcagggc
    22021 acggataaca gagccgcctt taccgataac atctttgatc ttgtccgggt tgatcttgat
    22081 ggtatggata cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc
    22141 cattacaccc aggatgtgca gacgcgcacc tttagcctgg ttcagtgcaa cctgcatgat
    22201 ctctttggtg ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga
    22261 acccgcaact ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag
    22321 tacaacgtag ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt
    22381 gattggcaca cctgcgtcca tcagcgccag agacgcaccg cacacggaag ccatagaaga
    22441 agaaccgttg gattcagtga tttcagacac aacacgtacg gtgtacggga atttgtccat
    22501 atccggcatt actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg
    22561 cttcggagaa ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa
    22621 caggaaggta tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcagt
    22681 acccagcgtt gcggtaacca gcgcctgcgt ttcaccacgg gtgaacagcg cagaaccgtg
    22741 agtacgcggc agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc
    22801 atcgatacgc ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatcgcgtg
    22861 cagaatttca cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat
    22921 ggtttcagat ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta
    22981 agcatcgctc agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac
    23041 cggctccggc tgccagtccc aacgcggttt accggcttct ttcaccagtt cattgatgtt
    23101 ctgaataaca acctgctgtt gttcatgacc gaacactact gcgcccagca tctggtcttc
    23161 gctcagcagt tcagcttcag attcaaccat cagtacagcg gcttcagtac ccgcaacaac
    23221 cagatccagt ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt
    23281 gatgtaacct acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag
    23341 tgctgcggaa gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac
    23401 cacggtggcg ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg
    23461 gcgatcaatc agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa
    23521 gaagctaccc gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg
    23581 gaagaagtcc tgacctggtt tggctttttt ctggccaaca acggtaacaa ataccgcggt
    23641 gtcatccatg ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag
    23701 agtcacggtg tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc
    23761 ctttctcttt cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat
    23821 cctcgcgact aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc
    23881 gcgaacctct gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc
    23941 tgccgtcagc ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta
    24001 gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag
    24061 gtagtcgagc agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg
    24121 atcttttttg tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc
    24181 tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt
    24241 agctgttgct tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac
    24301 gccgatctct aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg
    24361 acgttaagcg gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg
    24421 ccgggtattc aaccaccaga cgacgaggcg caacgcggcc ttcatcgtca atttcgccca
    24481 taccgataaa tttgccgttc tcaccttccg tgacgcgaac cagtccttcc agcggcgcgc
    24541 cagatgtacg aaccgggtta ccatttttga agtaaacaga agacgttaac ggaagattca
    24601 ccaccggata gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg
    24661 cagctggaat atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca
    24721 ccatccgttc aaccggatat ttacttaccg ccagacggcg caggtaaata acatgcgcgc
    24781 cacaaccgag tttctcaccc aggtcgtcaa tgatagtgcg gatataagtg ccttttgagc
    24841 agtgaatttc cagctccagc tcattgcctt catggcgaat aaacagcaat tcataaacgg
    24901 taatcggacg cgcttcacgc ggaacttcaa tgccctgacg cgcatattcg tacagttttt
    24961 tgccctggta tttcagtgct gaatacatcg atgggatctg ttcgatatcg ccacggaaag
    25021 tatccagtgc cgccgccagt tgttctgcac taaaggttac cggacgctct tcaacgattt
    25081 gtccgtcggc gtcagaagta tcagtacgct gtccaagacg cgcaatgacc cgatagcgtt
    25141 tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa atcggcaaca
    25201 tgccggtcgc cagcgggtcc agcgcaccgg tatgaccggc acggttggcg ttataaatcc
    25261 gtttcacttt ttgcagcgca tcgttgctgg acataccctg aggtttatcc agcaacaaaa
    25321 cgccgttaat gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc
    25381 cgggttaaca cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg
    25441 catcccttca accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg
    25501 catcgctttc cccagcaggc tgcggatgaa accagaagct tcctgcaacg ctttgatgcc
    25561 cgctttaact gcgtcttcat ctttgtcgtt gaggaaagtg acatacactt tggcatacgc
    25621 caggtcgcga gacatttcga caccggaaac ggtggtcatc atgcccagac gaggatcttt
    25681 aatttcacgc tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg
    25741 gcgaccaaat tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt
    25801 tttaaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgctagcg
    25861 tcgcaacagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat agaaccctaa
    25921 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    25981 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    26041 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcatagat aaccacgttg
    26101 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    26161 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    26221 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcgatcagg
    26281 ttatagatga cggagtagta acgtagatcc aggctttcgg attcaatcac tttacgtgca
    26341 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggatgccgc agccagggtg
    26401 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    26461 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    26521 aggacgatat tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    26581 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atagagtgca
    26641 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggaaca
    26701 ccggacaagc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    26761 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    26821 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    26881 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    26941 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cggtacccgc tttcgcagat
    27001 acgtgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    27061 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaaccacc
    27121 accggtacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg cattacgccg
    27181 tcgtcggcag caacaaccag gactacgatg tcagttgcct gcgcaccacg tgcacgcatt
    27241 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    27301 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    27361 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    27421 accggagcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    27481 agctcgtttt cacgacgcag gataactttg tggcccatct cttcagcaac cagctgtgcg
    27541 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    27601 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    27661 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    27721 gaacctttac gttttccgcc cttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    27781 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    27841 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    27901 gaagtagtga cgtgataatc gctggaatcc tcagtcggtt ccgcgttatc agtccatttg
    27961 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    28021 cgacgcgctt cttcttcggc tttacgcttg agctctgcag cttcctgctc acggcgggct
    28081 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    28141 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    28201 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    28261 tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt cagcctcttg cggatcgcgt
    28321 ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt tccaccggta
    28381 ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc cgggcctgaa
    28441 tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac agagtcgtca
    28501 gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac ggaggtctgt
    28561 cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc tgctacagtt
    28621 tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag tgctccggct
    28681 ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc ggcgagatct
    28741 tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc taccccttca
    28801 aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg ggcctgtgca
    28861 atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg ctcatcaagg
    28921 ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag cgtcgagaag
    28981 ccttcctcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt ggtgaaggtg
    29041 tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac ggtcatcacg
    29101 ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt acggccaatc
    29161 gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc ttcatccacc
    29221 acgatagaag caacgtctgc tggtgccatt gcgttaatca caaactgcgc cgggttatca
    29281 tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc ctgaacacgc
    29341 gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt ggttttcacc
    29401 gcgattttcg cacgagaacc cgggtcgcga gccgctgctt taatttcaat cacttcttcg
    29461 ccgatttctg gcacttcaat acggaacagt tcgatcagca tttccggctt ggaacgagtg
    29521 acgaacagtt gcgcgccacg cgcttccggg cgaacggaat aaagcacgcc acgaacgcgg
    29581 tcgccagggc ggaagttttc acgcggcagc atatcttcgc gcaggatcac ggcttcagcg
    29641 ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac gccggtgatg
    29701 atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc ttcacgcact
    29761 ttctgcacga taacctgttt tgccgtctgg gtagtgatac ggtcaaaggt aacagactca
    29821 atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg tgcggcttca
    29881 agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg acggaaggtg
    29941 tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt cgatctcttg ttcatatttt
    30001 ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc gcgaggtagc
    30061 gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat cgcgggcttt
    30121 tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat attactcagc
    30181 gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc taccgctttg
    30241 ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag agtcacctct
    30301 tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag acccggtgag
    30361 gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac agcacttacc
    30421 tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc aatatagatg
    30481 cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa acccagggcc
    30541 tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa gcccaccccc
    30601 aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca aaaaaccccg
    30661 ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg gagcactttc
    30721 cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat atttgaaaaa
    30781 ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga
    30841 gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgtggcaaat tctactcgtt
    30901 ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc gagacgggac gtaaaatctg
    30961 caggcattat agtgatccac gccacatttt gtcaacgttt attgctaatc acgtgaatga
    31021 atatccagtt cactttcatt tgttgaatac ttttgccttc tcctgatccc ccttaagcgc
    31081 attattttac aaaaaacaca ctaaactctt cctgtctctg ataaaagatg attaaatgaa
    31141 aactcattta ttttgcataa aaattcaatg agagcggaaa tccaggctca tcatcagtta
    31201 attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta ggtcaacgta
    31261 ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg atgcgacaaa
    31321 agggagcggt tccttatgca tatactgcaa acctgggcca gccagacgaa gaggattatg
    31381 atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg atcgactgcc
    31441 gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt cataacacca
    31501 ccggtggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact ggcaccatgc
    31561 tggttgctgc tatgaaagaa gatggcgtga atatctgggg tgacggcagc acctataaag
    31621 gaaacgatat cgaacgtttc tatcgttacg gtctgctgac caatgctgaa ctgcagattt
    31681 acaaaccgtg gcttgatact gactttattg atgaactggg cggtcgtcat gaaatgtctg
    31741 aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagct tactccacgg
    31801 actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc aactccagcg
    31861 tcaaaatcgt caacccgatt atgggcgtga agttctggga tgagagcgtg aagatcccgg
    31921 cagaagaagt cacagtacgc tttgagcaag gtcatccggt ggcgctgaac ggtaaaacct
    31981 ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt cacggtctgg
    32041 gcatgagcga ccagattgaa aaccgtatca tcgaagcgaa aagccgtggt atttacgagg
    32101 ctccggggat ggcgctgctg cacattgcgt atgaacgtct gcttaccggt attcacaacg
    32161 aagacaccat tgaacagtat catgcacatg gtcgtcagtt gggccgtctg ctgtaccagg
    32221 gacgttggtt tgattcccag gcgctgatgc tgcgtgactc tctgcaacgc tgggttgcca
    32281 gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat tcaatcctga
    32341 ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa aaaggcgact
    32401 cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaacctg gatatcaccg
    32461 atacccgcga gaaacttttc ggttatgcca aaaccggcct gctttcctcc tctgccgctt
    32521 caggcgtgcc gcaggtggat aatctggaaa acaaaggcca gtaattcgac atcaaccctg
    32581 cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga gcatcacata
    32641 atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt gtgcaatcac
    32701 ttcctcaagc gtttgcggct gttccggctt tgttaccccc atccacgcat taataaggta
    32761 attattgtaa gctgtcgaaa agatgttgtt ttccgttgtg cgatccacac catccccaag
    32821 aacggggcta taccagataa acatcggaac atgatatgcc tgctggctgg cctccctgcc
    32881 tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata
    32941 catgaccgag gcgcggcgat cttttaataa ttcaaaaacc tgacctagca aactatctgt
    33001 gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct
    33061 ggattgcgga taggcgctac aagccggttc atggcttcca tttaaatgaa gaacaatcag
    33121 tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca acaattcatc
    33181 aaatcctctg acataaactg tttccatggc acgcatggcg atactggtaa ctgctgtacc
    33241 attctgccga aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg cttgattagc
    33301 catattaata atgttgtcgg ggtaattatg aatgtcatgg ctcaaaacag aatcagcagt
    33361 taaagaaagg ggaaccgaca gcgcagtgta aggtgcaccg cttattgctt gattaaacag
    33421 tttgatctgt tttctttgtg cttcgacttg cggtgtcgta gagcgtgtat atccatacaa
    33481 agacatattg tcgacacgta cagactcccc cacaatcaac acgtaggtat caatacctgt
    33541 atccctgact gataattgaa aatacggcac tgtgtttgca attgagagta atctttgatg
    33601 ctctttcgct gctaaagcaa aataattgag attgaaaaac ggcgtatagg tagcaaatcg
    33661 cgacgctagt atatatggac tgaacgcatt tttatttttg gcatctttat aagcaaattg
    33721 acaagcggaa aataaacttc ccgaaatgac aatcagcaat aatattcctg tcactttttt
    33781 tgtcggcaag gaaacatcat attttattat tacagccaaa aaaagaaggg ataaaaaggc
    33841 aaaagcacat agataagggc tatacattcc cagcattttg gcaacttcat ccggatcact
    33901 ctgcagcaca ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac accatgaata
    33961 gaggctaata ctcatgtctg ccgcagtcag taacacaaat ggtgcggcaa caagcaggcg
    34021 aagcggaagg gatgatatac gtttaacaac caggatggtc agaacgaaga acagcaatcg
    34081 ctgaaccata tgcgctccgg aagaggcgac taagttcgta ataccaaaaa caataacact
    34141 aagatacaac aaccaatgag atttaaaact tctagcaaat ttgttgaata ctgtcatcca
    34201 gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgtgcaattt tatatcagac
    34261 atgtttttca actctgatcc atatcagaac gaaacactgt tagttttttc tttttaaatg
    34321 atgcggccat tgcgttctca gtaattaaat aacattatca atgcgtctct aaacattacg
    34381 cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg agaagtaaac
    34441 atatccgtat tcgtaccgga actggagtta acacaataaa gtgccaatta tgtcagcagt
    34501 agggaaaagt ttataacgaa ggcgtaccgg caccatcgtt tcaaggtgcc agctacgagt
    34561 aaagcaactg gacgagatac agatacctga caaccattcc tcagaccagg accaaaacga
    34621 aaaaagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg gacttgaacc
    34681 cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc caccacctcg
    34741 gcacggaaac tactttttag ttcgggatat cgctggtcgg cttagccgga gcagctggct
    34801 gagtttgttc ggttttcgcc ggcgcactca ggttttccca ttcgctacct ttattggttt
    34861 tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt gccagcaacg
    34921 ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta gcggaagcgc
    34981 ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg atcagaccaa
    35041 caaggccaat tgccacaata aggaaaacta ccaaaagagc ttcgtacata atcaacctgt
    35101 tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt tttaatcttt
    35161 cccactgaag cgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg gcaattttat
    35221 cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgccga gaaaaaaggc
    35281 gacaatcgcc acctttttag ccagttatct aacgctttaa acggctttta ctgcatcggc
    35341 gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat
    35401 taacggttcg gtgccggatt tacgcagcaa cacgcgtcca cggttgccca gcgccgcttc
    35461 aacctctgcg gtcacagctt tgactgattc atgctccagc ggatcgccgc tacctgcggt
    35521 gtaacgcacg tttaccagga tttgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag
    35581 gctcatatgg ttacgcgcca tcgccgccag cacctgcaaa ccagcaacga tgccgtcacc
    35641 ggtagtggtt ttatccagca ggatcacatg accggaattc tctgcaccga tacgccagcc
    35701 tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat
    35761 tcctaactgt ttcaacgcca gttcaagccc catgttgctc atcaatgtac ccacagcgcc
    35821 accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga tctgatcgcc
    35881 atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc
    35941 aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc
    36001 ccccacttcg gcattgatgt ttacaccgtt cggctcacaa ccgatagcga taacgttcgc
    36061 ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg cacaatccac
    36121 cacaatcttc agttcactaa ggctaagttc gttcgggaac gtggctttgc aaaactcgat
    36181 atagcgaccc gcagcatcaa cgatacggct ggctttaccc agttctgccg aatcaacgca
    36241 gctgatctcc ttttccattt ccgcttcgat ggcctcttct accgcatccg gcagtttggt
    36301 gccgtcgata gagaagaatt taatgccatt atcgtagaac gggttatgcg atgcagatat
    36361 cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg gtgttggcat
    36421 cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca gtgctgactc
    36481 cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg agccgtggcg
    36541 cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcagggg tgatcggcgc
    36601 atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac tcatagcgtt
    36661 tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg gtttctttga
    36721 cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca caggccagac
    36781 taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc gattttcgtg
    36841 acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc gccagtaatg
    36901 aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac aatttctctt
    36961 ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga ttcacttctg
    37021 caaagacatc gtcatacttc ggagcttcct gcatggtttt gggatttccc tgcatatgca
    37081 tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt tcggaaaggg
    37141 agcggatatc attaataatg tgagcgccaa cttttgctga ctcacggatg acttctggtt
    37201 tggatgtatc gaccgagatc cagacttcga agcgttgagc aattgcctca accacaggaa
    37261 taacacgttg caactcttct tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc
    37321 caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag attcgcatgt ttcaccgcat
    37381 ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc
    37441 ccattacgtg aggatggcta aggtccagtg aagtaccctg ggcaaagagt ttcatgatgt
    37501 tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt tttcggtaca
    37561 aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca tggtgttacc
    37621 cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac cattgtcgcc
    37681 agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat cgcgacgtgc
    37741 catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag catctttcat
    37801 cgcatgcaga atatccatat tgtcggtcag aagctgacgc gcacgattat agttacgttc
    37861 aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc
    37921 tttcgctacg ctacggccga ggaacacttc accttcttct tccgcgtaca gcagtggacc
    37981 caatttctca gagaagcccc actgagtcac catgttacgt gccaggttgg tcgcaacttt
    38041 aatatcgttg gacgcaccgg tagaaacatg ttccggcccg tagatgatct cttctgccag
    38101 acggccgccg tacagcgtag aaatctggct ttccagtttc tgacggctgg cgctgattgc
    38161 gtcgccctca ggcaggaaga aagtcacacc caacgcacga ccgcgtggga taatcgtcac
    38221 tttgtgcacc ggatcgtgct ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg
    38281 gtaagccgtc gattctttct gcgcttccgt catcaccatg gagcgacgtt ccgcacccat
    38341 catgattttg tctttcgctt tctcgaactc aaccatcgac acaacgcgtt tgttgccacg
    38401 agcagcgaac agtgccgctt cgttcaccag gttcgccagg tcagcaccgg agaaaccagg
    38461 agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg
    38521 aaccttcagg atctgctcac gaccgcgaac atctggcaag ccaaccacaa cctgacggtc
    38581 gaaacggcca ggacgcagca gcgctgggtc gagaacgtcc ggacggttag tcgcggcgat
    38641 aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct ggttcagagt
    38701 ctgttcacgt tcatcgtgac caccgcccag accagcgcca cgctggcggc ctacggcgtc
    38761 gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga acatgtcacg
    38821 aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg agatagtaaa
    38881 gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc agcgttttac cggtacccgg
    38941 aggaccgacc atcaggacgc ctttcgggat cttaccgccg agtttctgga agcggctcgg
    39001 ctcgcgcaga tactcaacca gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac
    39061 gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa
    39121 cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga tccagacacc
    39181 aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc ttggttcttc
    39241 aggcggttca ccgacaacct tgacgttctt ggtcaacagg ttatccagta gtttcggatc
    39301 ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt tgatttcacg
    39361 tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga aggtagagta
    39421 atccacctta cggccattag actcgctggg cccaaagctc tggaatactg acatcagcac
    39481 aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg gattaacctc
    39541 ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc tttcaaactt
    39601 tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca cttcccgcga
    39661 acgtgcacga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg agcgaatttc
    39721 ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac tgccacctgg
    39781 cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg cacgggggat
    39841 atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa cctggacttt
    39901 gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa aatcgccctg
    39961 aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga tgatgcggcc
    40021 tttgccgcca atttgggtga ccacatattg tgaccaacca cccggagcag caccaaggtc
    40081 gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat caagtttaaa
    40141 ccaggcacgg gaacgtaacc cctttttctg tgcctgttga acatatttat cgctaaagtg
    40201 ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt aactttccca
    40261 tggggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg gtgatatatg
    40321 ggagatggcg gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc
    40381 tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag ccagttgttc
    40441 tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa gcgttagagc
    40501 accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaactaaa accttgatcg
    40561 tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg
    40621 tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag
    40681 ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc aaaatgccac
    40741 gctctgttcg ttgataaaag gccgcatagc ggcctttttc ctttctttac aatacatcaa
    40801 catcttgagt attgggtaat tcttacaggt attccacctt aattacttca aattctactt
    40861 cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc aggccacgag
    40921 caataggtga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca tcgccaacga
    40981 tgcgataagt ctgttcttcg tcagaatcga gattcagcac cgttacggta gcaccaaaaa
    41041 taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttc gacagcttgg
    41101 cttcgatgtc tttaatacgg ccttcgcaga aaccctgctg ttcacgagct gcgtggtatt
    41161 cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca atgatttcag
    41221 gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg
    41281 tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg caagtcttca
    41341 cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga agcaaaaaaa
    41401 taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatag tcattttacc
    41461 ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag tatgacggct
    41521 cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc ggattgacca
    41581 gctgtatagc gttcagtgtt caggccgcaa atgttgatga gtacattact caactccccg
    41641 ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc gtcggccccc gctattgatt
    41701 accacagtca gcagatggcg ctgcccgcca gtacccagaa agtgattact gcgctggcgg
    41761 cgttgattca actcggcccc gattttcgtt ttaccacgac gcttgaaacc aaaggcaatg
    41821 tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa
    41881 aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg
    41941 atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc cccggctggc
    42001 catggaatga catgacacaa tgctttagcg ctccgcctgc cgccgccata gttgaccgca
    42061 actgtttctc cgtctcgctc tacagtgccc caaaacctgg tgatatggct tttatacgcg
    42121 tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc cgtggttctg
    42181 ccgaagcgca atactgcgaa ctggatgtcg taccaggcga cctgaaccgc tttacgctga
    42241 cgggatgcct gccacaacgt tctgagccgc tcccgttggc ttttgccgtg caggatggag
    42301 ccagctatgc cggtgcaatt ctgaaagatg agttaaaaca ggcgggtatc acctggagcg
    42361 gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc agtaaacagt
    42421 cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcggacaac atgatcgccg
    42481 atacggtttt ccgcatgata ggccatacgc gcttcaatgt gcctggaaca tggcgggccg
    42541 ggtcggacgc tgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt ggaaacacca
    42601 ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc accatgatgc
    42661 aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc atgctgccgc
    42721 tggcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg
    42781 gaaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg ggattcatta
    42841 ccacagcgag cgggcaacga atggcgtttg tacaatatct ttctggctat gcagtagaac
    42901 cggcggatca gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt ttgtataaag
    42961 atatttatca gaacaattag tcaaaaagaa accccggcac atggccgggg ttttgctgat
    43021 taacgcttgt aaatgaactc aacgccttct tcgtcgtctt cgtcccagtc gtcatcccag
    43081 tcctcatcat cctcttcagc aatctcttca agctgctggc gatgataatc atcccacatg
    43141 aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata
    43201 aaggtcatca catcccagca gagatctttc acgcccagtc cactcgccgc agagatcaga
    43261 taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc ttcggcttct
    43321 gccttatcca gcagatcgat cttattgaag actaaccaac gcggtttcgc cgccagatcc
    43381 tggctgtatt tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg
    43441 tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg
    43501 aagcgaatgc ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca
    43561 acaacgaagc tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta
    43621 aacggataat ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta
    43681 cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc
    43741 tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat
    43801 ttgaaacggg tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg
    43861 tgtttggtca tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc
    43921 ggcactttaa ttgtgacgtc tttaccgcgc ttaccggtac agtcgcggct tgcgccattc
    43981 tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc
    44041 tcgtcggctt ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct
    44101 ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg
    44161 accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg
    44221 cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg
    44281 gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac
    44341 atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta
    44401 aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct
    44461 tccgccaggg cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc
    44521 tgccagtggc taagctgcgc tatcactcca ggctttgcca gagggaagag cgcaattgta
    44581 cataaagtgt acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt
    44641 tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag
    44701 gtgtaatcgg ttagcttggt aaatatctcg accagactgg tgttaaaaaa catcaccagg
    44761 ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata
    44821 aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc
    44881 ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc
    44941 gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc
    45001 ttcttcaccg caagaatggc acccaggcca atactcgcca tcaagaaacg gtaaaacacg
    45061 attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag
    45121 caaattgctg tggtgagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat
    45181 tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga
    45241 atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact
    45301 tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact
    45361 ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg
    45421 tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg
    45481 aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca
    45541 gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt
    45601 tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa
    45661 acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc
    45721 cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt
    45781 cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac
    45841 cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg
    45901 cttccgcgca caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg
    45961 aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa
    46021 cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaatcac cctatattcc
    46081 taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc
    46141 ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa
    46201 tgcggcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta
    46261 catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc
    46321 tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt ttatccacac
    46381 ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac
    46441 cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg
    46501 cgcgttccag atgatgacca gcctcggttc gctcaaagtg ctggaagtca tgtcagaagc
    46561 cgtaaacgtc atcgcagagg gtgaagttct gcaactgatg aacgttaacg atccggacat
    46621 cactgaagaa aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc
    46681 cgcgcagtgt tccggaattc tggcgggctg tacgccgaag gaagagaaag gcctgcagga
    46741 ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc
    46801 cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct
    46861 gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc
    46921 catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg
    46981 tggatctctt gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc
    47041 gttacaggtg ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc
    47101 tgttcaacgc gatcgttaat cccctcccct catcccgcgc agtgcgcggg atgattccag
    47161 taaactccat aagaatactt attcagcgta ataatgcctg aaaaaccagt gtatacatct
    47221 ttatatattc tgaatattca cactctttac aggaactttt tagagcaatc ggccatcagg
    47281 agtatagtga tgctcgacag aagaagtgtt ctgaatgaaa gcgactaact taaggagtga
    47341 ggaaagatgg aaagtaattt cattgactgg catcccgctg acatcattgc gggtttgcgc
    47401 aagaagggaa cgtcaatggc ggcggaatct cgcagaaatg gtttgagttc ctcaacgctg
    47461 gcgaacgcat tatcgcgccc atggccgaaa ggagagatga ttattgcgaa agccctggga
    47521 actgacccct gggttatatg gccatcacgc taccatgatc cgcagaccca tgagtttatc
    47581 gacagaacgc agttgatgcg tagctacact aaaccgaaaa aatgagtggt ctggcggtag
    47641 ccccgcgaac ggggctgcca gctctcagac gattactcgc ctttcacacg ctcaatattt
    47701 gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc cacgatcgat gtgataaata
    47761 cgatcaacca ccgtcgtccc ttccgcaata cagccagcca gcaccaggct tgctgatgca
    47821 cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt caacaccgtg acaaataacg
    47881 gtattgcttt cgatttcggc gtgcgcgccc atacggctca gctctggcac atgcataaag
    47941 cggttttcaa aaaccgtttc ggtgatgaat ccggtccctt ctgccaccag gttcaacagc
    48001 gtgaactggg cctgcatatc ggtcgggaat gccggatgcg gcgcagtacg tacgttaaca
    48061 gccttcggac gtttgccatg catatccagg ctaatccagt cttcgccgac ttcgatgtcc
    48121 gctccagcat cacgcagttt cgccagcacg gcgtcgagag tatctggctg cgcgttacgg
    48181 cagataattt tgccgcgaga gatcgccgcc gccaccagga aagtaccggt ttcgatacga
    48241 tccggcagca cgcgatagac accgccgcct aaacgttcca caccttcgat gacgatacga
    48301 tcggtgccct gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg
    48361 atttccggtt cacgcgctgc attttcaata atcgtggtgc cttccgccag ggttgcagca
    48421 cacatgatgg tcaccgttgc gccaacgctg actttatcca tcacgatatg tgcgcctttc
    48481 agacgaccat cgacggaagc tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat
    48541 tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca accgccaggt
    48601 agtgaaactt gcccctgacc aaagcgcgct accagcggcc ccagtgccca gatagaagca
    48661 cgcatggttt taaccagatc gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata
    48721 tgcacagaac cattacgttc tactttcgca cccagctggc ttagcagctt cattgatgta
    48781 tcgacgtctt tcagtttcgg gacgttctgg atctctaccg gttcttccgc cagtagagcg
    48841 gcaaaaagga taggcagagc agcattttta gcgccggaaa ttgtgacttc gccctggagc
    48901 ttcgttggcc cctgaacacg aaatttatcc atttagtttg ttctcagtta acaattcata
    48961 tccgctaccg gcgaatcgcc catagctcaa aagccgttca gtttgcgatc gcgcgcccac
    49021 tccgcagggg tatacgcttt gatcgacaca gcatgaatgc ggttatccgc aatatattcc
    49081 atcagcggac catagaccgt ctgctgtttt ttaacccgac tcatgccgtc aaacaactca
    49141 cccacggcaa taacctgaaa gtggctgcca tcgccggaaa cgtggacttc ctggagggag
    49201 agagcgttca tcaacacgct ctgaatttca ttattttcca tgggatcttc aatcatcagt
    49261 taataaacca gcgaaacatc ttagagcaaa gttgcgctgg cataaataag caaaaagcct
    49321 cgctgataaa tcagacaagg ctcgacttgc aggcaggttt gccggacagg cggttaacgc
    49381 catatccggc ctgaaaaaat ttaacggggc agaacatcag caggcaaatt atacaatttc
    49441 gccagggtat acactttgtc gtttaccccc tgaagcgtca cattgttgcc ctgctttttc
    49501 gccagatcga taagatggag cagcagtgcc agtccacccg tatccacgcg ggacacacgg
    49561 ctaagatcga tgcaggtaac ccccttcacc gcttcctcac gcatttccca aagcggtagc
    49621 agaacgtcct gatccagctc tccggataac gccagcgtgt cacccgtctg catccagctc
    49681 agtgactcgc tcattatttt ttctcttcca gagtgatttt ctgttgagaa atcgatttca
    49741 gttgcgcagt caggccgtcg atacctttgg tacgcagcag cgttccccac tcgttttgtt
    49801 tggtggtgat catactgacg ccttcagcaa tcatgtcgta agcctgccag ttgcccgtct
    49861 gggagttttt acgccactgg aagtccagac gcaccggcgg gcggccattc gggtcaataa
    49921 tggtaacgcg aataggcaca atggttttat cacccagcgg ctgttctggc gcaatctgat
    49981 aggtttgacc gtgatacatc gccagcgcct gaccgtaagc ctgcttcagg tactcacgga
    50041 aagcggcaaa gtaggcttca cgttgagctg gggtcgcact cttgtaatac tggcccagca
    50101 ccagcgcacc ggcgtatttc acctgtacgt atggcagcag ttcctgatca acaatggtac
    50161 gcagataatc cgggttggcc cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt
    50221 tctgcgccgc ctcgtccatc agcttatacg gattggtctg gtctgccgcg gttgccgcac
    50281 tcagcggtgc aatcaccagc aaagcgacca tcattaaacg tttaaacatg cgtcggttct
    50341 cctgaaatta tttcgttgta cccacaggtt cagtggtttc attattacct ggcgcagcag
    50401 ctggcgcatc gccactattc ttattgtcat cgcctttact accgtaaagg aactgaccaa
    50461 tgagatcttc cagcaccatc gcagacttgg tgtcctgaat tgtatcgcca tccttcagga
    50521 tagcagtccc cagttccggg tcttcaaaac cgacgtttaa tgccagatat tgttccccca
    50581 gcaggccgga agtacgaatg ctcagcgaac tggtatcagg aatatggtta taacgttgtt
    50641 caatttccag cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata tccgccaccc
    50701 ggcccacaac aacgccacca atactgaccg gagagcgcgc tttcaggccg ccaatgttat
    50761 cgaacgtcgc ataaagcgta taggtcggtt cagtacgtat tgacgtcacg ttcgccgcct
    50821 tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa aaagataccc acccaaattt
    50881 catttttttt cgtttgcatg aactcaattc ccaaacatca atgcggtcag cacaaaatcc
    50941 agccccagaa cagccagaga cgagtggaca acggtgcgag tggttgcccg gctaatcccg
    51001 gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg
    51061 aacaccacgc tcttaatcag acagttgacc agatccatac gccagtcgac ggcattttgc
    51121 attgccgacc agaagaaccc gctatcaatg cctttccaac tgacgccgac cagcgatccg
    51181 ccccagatcc ccacggcgac gaagataacc gtcaacagtg gtaatgaaat aaccccagcc
    51241 cagaaacggg gagaaataac ccgacgcagc ggatccaccg ccatcatctc catactggag
    51301 agttgctctg tagcgcgcat caggccgatt tctgcggtta acgccgaacc agcacgcccg
    51361 gcaaacaaca gcgcggcaac caccggcccc agttcacgca gtagcgataa cgccaccagc
    51421 atacccagac tggtttccgc actataagtg gtcagaacca gataaccttg cagccccaac
    51481 accattccga tgaacacgcc agaaactaca ataatcagca tcgacaggac gccgacatta
    51541 tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca
    51601 ttgaataaca ttaacccggc ccgcccgaac gttctcaggg ttttaatccc tttatgtccg
    51661 agcgacgcca gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg
    51721 ataatcgccg gcaggatagc ggaacggaac aggcccgtca gctatcccgt ccagaaactg
    51781 acgtacgcgc ggatcaggat tcgcctgcaa cgccttggca ctgccatgag cgacaatttt
    51841 tttgtccgcc aggatccagg cgtgatccgc aatacttaac acttccggca catcgtgaga
    51901 aaccaccaca caagtcacgc ccagcgcgct gttcagctca gaaatcagct tcaccagtac
    51961 gcccatggta atgggatctt gcccaacaaa gggctcatca aacatgatga gatccggctc
    52021 cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg
    52081 cattagttta gccgctccac gcagccccac ggcctccagc ttcatcatca ccgtactatg
    52141 caacaatggc gcgggaagtt gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac
    52201 gttcatatca gtgaacaacg ccccggactg aaataacatg ctcatccgtt tgcgcactgt
    52261 atacaggcgc gaacgagaca tcgccggaat attctcacca tcgaaaagga tctcaccatg
    52321 atctggtgcg atttgcccgc caatcagacg gagtagcgtc gttttaccga tgcccgatgg
    52381 ccccatgatc gccgtgatct tccctcgcgg cacggtcagg gaaatattat cgaagatgca
    52441 gcgattgcca cgcgtaaaac tgacatcgcg catatcgact aaattcgcca cagactgctc
    52501 cataattcac ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt taaccttgaa
    52561 cccaacatat ttacagaata ttacccgccg tggttagcga aagctggcat ttgttttact
    52621 ttttagccgc ataaagtcaa aattaagaat ccgtta
//
LOCUS       DAZGCQ010000023        52291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000023 DAZGCQ010000000
VERSION     DAZGCQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.023"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(604..688)
                     /locus_tag="ACHK07_001450"
     tRNA            complement(604..688)
                     /locus_tag="ACHK07_001450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:Leu,seq:caa)
     gene            884..1904
                     /gene="ahr"
                     /locus_tag="ACHK07_001451"
                     /pseudo
     CDS             884..1904
                     /gene="ahr"
                     /locus_tag="ACHK07_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            complement(1908..2471)
                     /gene="idnK"
                     /locus_tag="ACHK07_001452"
     CDS             complement(1908..2471)
                     /gene="idnK"
                     /locus_tag="ACHK07_001452"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO8101804.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPHADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            2688..3719
                     /gene="idnD"
                     /locus_tag="ACHK07_001453"
     CDS             2688..3719
                     /gene="idnD"
                     /locus_tag="ACHK07_001453"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO8101805.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3743..4507
                     /gene="idnO"
                     /locus_tag="ACHK07_001454"
     CDS             3743..4507
                     /gene="idnO"
                     /locus_tag="ACHK07_001454"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO8101806.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4570..5889
                     /gene="idnT"
                     /locus_tag="ACHK07_001455"
     CDS             4570..5889
                     /gene="idnT"
                     /locus_tag="ACHK07_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO8101807.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            5956..6954
                     /gene="idnR"
                     /locus_tag="ACHK07_001456"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             5956..6954
                     /gene="idnR"
                     /locus_tag="ACHK07_001456"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO8101808.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7032..8534
                     /gene="yjgR"
                     /locus_tag="ACHK07_001457"
     CDS             7032..8534
                     /gene="yjgR"
                     /locus_tag="ACHK07_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO8101809.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASNEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8695..9777)
                     /gene="lptG"
                     /locus_tag="ACHK07_001458"
     CDS             complement(8695..9777)
                     /gene="lptG"
                     /locus_tag="ACHK07_001458"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8101810.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9777..10877)
                     /gene="lptF"
                     /locus_tag="ACHK07_001459"
     CDS             complement(9777..10877)
                     /gene="lptF"
                     /locus_tag="ACHK07_001459"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8101811.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10940..10990)
                     /gene="ytgA"
                     /locus_tag="ACHK07_001460"
     CDS             complement(10940..10990)
                     /gene="ytgA"
                     /locus_tag="ACHK07_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO8101812.1"
                     /translation="MSIIILATISTTQENK"
     gene            11144..12655
                     /gene="pepA"
                     /locus_tag="ACHK07_001461"
     CDS             11144..12655
                     /gene="pepA"
                     /locus_tag="ACHK07_001461"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO8101813.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13009..13452
                     /gene="holC"
                     /locus_tag="ACHK07_001462"
     CDS             13009..13452
                     /gene="holC"
                     /locus_tag="ACHK07_001462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO8101814.1"
                     /translation="MKNATFYLLDNDITVDGLNAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13452..16307
                     /gene="valS"
                     /locus_tag="ACHK07_001463"
     CDS             13452..16307
                     /gene="valS"
                     /locus_tag="ACHK07_001463"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO8101815.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEYN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16363..17559)
                     /gene="yjgN"
                     /locus_tag="ACHK07_001464"
     CDS             complement(16363..17559)
                     /gene="yjgN"
                     /locus_tag="ACHK07_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO8101816.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKRFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            17752..18255
                     /gene="yjgM"
                     /locus_tag="ACHK07_001465"
     CDS             17752..18255
                     /gene="yjgM"
                     /locus_tag="ACHK07_001465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8101817.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPVIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(18301..18717)
                     /gene="rraB"
                     /locus_tag="ACHK07_001466"
     CDS             complement(18301..18717)
                     /gene="rraB"
                     /locus_tag="ACHK07_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO8101818.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            18879..19883
                     /gene="argF"
                     /locus_tag="ACHK07_001467"
     CDS             18879..19883
                     /gene="argF"
                     /locus_tag="ACHK07_001467"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO8101819.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
     gene            complement(19940..21766)
                     /locus_tag="ACHK07_001468"
     CDS             complement(19940..21766)
                     /locus_tag="ACHK07_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101820.1"
                     /translation="MSKISDLNYSQHITSADNFKPKNEVLNTWCVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSSKVIIEISLSTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDDS
                     SLHLITNKVLACYQREANKEIARLLNNHQKLNNLQKLNNLQKLNNIQELNNSQELNNS
                     QELNNSQALNNSQELNNPQELNNSQESNDSQELNNSQVSCKGSVDSTITDLLETTLNN
                     ALLAIRNEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDNEINDI
                     KVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYINDVRILDWCFNKIMQYFGDSEK
                     IKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLV
                     AYVNDFNLSNQEKSRLILRLFDDVKLLFDEVPVSILVNDIFLKDFFMKQPDFAKWYFY
                     QLLKKYEGEQLYLNELGYVYGNEEKTNEIVKKYPGYVVKIFEEKMGNELKIRTRMMET
                     LRDGKINIYDYINEKQLEKLNPPQDLRSAIEKIGWKNRPITA"
     gene            complement(21889..22341)
                     /gene="tabA"
                     /locus_tag="ACHK07_001469"
     CDS             complement(21889..22341)
                     /gene="tabA"
                     /locus_tag="ACHK07_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO8101821.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(22486..23079)
                     /gene="bdcR"
                     /locus_tag="ACHK07_001470"
     CDS             complement(22486..23079)
                     /gene="bdcR"
                     /locus_tag="ACHK07_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8101822.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            23150..23863
                     /gene="bdcA"
                     /locus_tag="ACHK07_001471"
     CDS             23150..23863
                     /gene="bdcA"
                     /locus_tag="ACHK07_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8101823.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDHL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            23994..24389
                     /gene="yjgH"
                     /locus_tag="ACHK07_001472"
     CDS             23994..24389
                     /gene="yjgH"
                     /locus_tag="ACHK07_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8101824.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDITSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            24670..24804
                     /gene="pyrL"
                     /locus_tag="ACHK07_001473"
     CDS             24670..24804
                     /gene="pyrL"
                     /locus_tag="ACHK07_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO8101825.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            24808..25743
                     /gene="pyrB"
                     /locus_tag="ACHK07_001474"
     CDS             24808..25743
                     /gene="pyrB"
                     /locus_tag="ACHK07_001474"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO8101826.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            25756..26217
                     /gene="pyrI"
                     /locus_tag="ACHK07_001475"
     CDS             25756..26217
                     /gene="pyrI"
                     /locus_tag="ACHK07_001475"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO8101827.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            26290..26676
                     /gene="ridA"
                     /locus_tag="ACHK07_001476"
     CDS             26290..26676
                     /gene="ridA"
                     /locus_tag="ACHK07_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO8101828.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(26882..29578)
                     /gene="mgtA"
                     /locus_tag="ACHK07_001477"
     CDS             complement(26882..29578)
                     /gene="mgtA"
                     /locus_tag="ACHK07_001477"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO8101829.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(29719..29772)
                     /gene="mgtL"
                     /locus_tag="ACHK07_001478"
     CDS             complement(29719..29772)
                     /gene="mgtL"
                     /locus_tag="ACHK07_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO8101830.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29957..30904
                     /gene="treR"
                     /locus_tag="ACHK07_001479"
     CDS             29957..30904
                     /gene="treR"
                     /locus_tag="ACHK07_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO8101831.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            31023..32444
                     /gene="treB"
                     /locus_tag="ACHK07_001480"
     CDS             31023..32444
                     /gene="treB"
                     /locus_tag="ACHK07_001480"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO8101832.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            32494..34149
                     /gene="treC"
                     /locus_tag="ACHK07_001481"
     CDS             32494..34149
                     /gene="treC"
                     /locus_tag="ACHK07_001481"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO8101833.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            34543..36681
                     /gene="nrdD"
                     /locus_tag="ACHK07_001482"
     CDS             34543..36681
                     /gene="nrdD"
                     /locus_tag="ACHK07_001482"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO8101834.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            36871..37335
                     /gene="nrdG"
                     /locus_tag="ACHK07_001483"
     CDS             36871..37335
                     /gene="nrdG"
                     /locus_tag="ACHK07_001483"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO8101835.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(37380..37766)
                     /gene="cybC"
                     /locus_tag="ACHK07_001484"
     CDS             complement(37380..37766)
                     /gene="cybC"
                     /locus_tag="ACHK07_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO8101836.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(37950..39302)
                     /gene="pmbA"
                     /locus_tag="ACHK07_001485"
     CDS             complement(37950..39302)
                     /gene="pmbA"
                     /locus_tag="ACHK07_001485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO8101837.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            39396..39947
                     /gene="yjgA"
                     /locus_tag="ACHK07_001486"
     CDS             39396..39947
                     /gene="yjgA"
                     /locus_tag="ACHK07_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO8101838.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(40103..41476)
                     /gene="mpl"
                     /locus_tag="ACHK07_001487"
     CDS             complement(40103..41476)
                     /gene="mpl"
                     /locus_tag="ACHK07_001487"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO8101839.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            41652..42650
                     /gene="fbp"
                     /locus_tag="ACHK07_001488"
     CDS             41652..42650
                     /gene="fbp"
                     /locus_tag="ACHK07_001488"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO8101840.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(42683..43642)
                     /gene="yjfF"
                     /locus_tag="ACHK07_001489"
     CDS             complement(42683..43642)
                     /gene="yjfF"
                     /locus_tag="ACHK07_001489"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO8101841.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLISEESIPINHPVYDTLSSLVWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQVGYALAGVGVELDAIASVVIGGTLFGVAIQGLIQTYINFDGTL
                     SSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(43629..44651)
                     /gene="ytfT"
                     /locus_tag="ACHK07_001490"
     CDS             complement(43629..44651)
                     /gene="ytfT"
                     /locus_tag="ACHK07_001490"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO8101842.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(44665..46167)
                     /gene="ytfR"
                     /locus_tag="ACHK07_001491"
     CDS             complement(44665..46167)
                     /gene="ytfR"
                     /locus_tag="ACHK07_001491"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO8101843.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQPISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIEMLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(46307..47263)
                     /gene="ytfQ"
                     /locus_tag="ACHK07_001492"
     CDS             complement(46307..47263)
                     /gene="ytfQ"
                     /locus_tag="ACHK07_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO8101844.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            47573..48103
                     /gene="ppa"
                     /locus_tag="ACHK07_001493"
     CDS             47573..48103
                     /gene="ppa"
                     /locus_tag="ACHK07_001493"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO8101845.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(48183..48533)
                     /gene="chpB"
                     /locus_tag="ACHK07_001494"
     CDS             complement(48183..48533)
                     /gene="chpB"
                     /locus_tag="ACHK07_001494"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO8101846.1"
                     /translation="MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(48527..48778)
                     /gene="chpS"
                     /locus_tag="ACHK07_001495"
     CDS             complement(48527..48778)
                     /gene="chpS"
                     /locus_tag="ACHK07_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO8101847.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(49068..50063)
                     /locus_tag="ACHK07_001496"
     CDS             complement(49068..50063)
                     /locus_tag="ACHK07_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8101848.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIVTLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(50130..50423)
                     /locus_tag="ACHK07_001497"
     CDS             complement(50130..50423)
                     /locus_tag="ACHK07_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8101849.1"
                     /translation="MDIPQKIRVMRKSEDLTQREFADMIGISYSALTSYEYGRNLPGL
                     EITIKLFKHPRFSKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            50539..50964
                     /locus_tag="ACHK07_001498"
     CDS             50539..50964
                     /locus_tag="ACHK07_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO8101850.1"
                     /translation="MQESTQNAPESLCGTRCDHQSEISEAEHLKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGLRNKRISKKAA"
     gene            complement(51046..51483)
                     /locus_tag="ACHK07_001499"
     CDS             complement(51046..51483)
                     /locus_tag="ACHK07_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101851.1"
                     /translation="MSIPSNLDVFNETVGKTFAFLYETFPRKVTIDICALTGVSELTF
                     EEDRSSRRQLVEALELRRDSIAWLVDAGYVSAKSSPPHYFSDAVLTAKGLELLKLDPE
                     SLKQSFGDKLVDAAKSGSMDAIKSTASSALTTGLSFAFKSLLG"
     gene            51482..51676
                     /locus_tag="ACHK07_001500"
     CDS             51482..51676
                     /locus_tag="ACHK07_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101852.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            51702..52037
                     /locus_tag="ACHK07_001501"
     CDS             51702..52037
                     /locus_tag="ACHK07_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8101853.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATVTTEIHATPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLISGL
                     RFQYPGSKH"
     gene            52056..>52291
                     /locus_tag="ACHK07_001502"
     CDS             52056..>52291
                     /locus_tag="ACHK07_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO8101854.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVA"
BASE COUNT        13386 a        13358 c        12922 g        12625 t
ORIGIN      
        1 ttataaccaa tgaaatctac aacaagcaac tgatggataa tccgatattg gtcgcattta
       61 atggcatggt agcaaagttt tcatcacata cctcctttgc accagaataa acgccagaga
      121 tctgaaattg cgagtacact acagaaattg tactgccggt cccatcagca taaaaccata
      181 gttacacagg taatcatcat tattcgccaa gataataaat attattcggt agaatagcgt
      241 tctcaggtgg tcagtaaaat tgtcttcaac aagatgtctg cgcatctcgt ctgctagccc
      301 tacataactg ctctcgaccg aaactattta ttgaatgttc accaaggtgg gcgtggagac
      361 aaaatgacgt cttttgatat tagctacctg agccacatgg tcaaaacgtt caagtagcat
      421 agcgaaacca ccctgcccac gtaatgtgga cgatgcagct tccagccaga gcaaacttgg
      481 gaccatcgac tacaaaatga agaatactga actgcttttc tggcaggcct atatactgca
      541 cggatatggg tgatttcaga cacaaaaaaa gccgcccttg agcgactcga tttgcatacg
      601 gtgtggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa
      661 ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt
      721 atacctgtcg cacgccacca tgcaagcgcc agcttgctcg cacctcgcta agtgctgaaa
      781 ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca ctccaggcac cccgccctgc
      841 catgctctac acttcccaga caacaccaga gaaggaccaa aaaatgtcga tgataaaaag
      901 ctatgccgca aaagaagcag gcggcgaact ggaagtttat gagtacgctc ccggtgagct
      961 gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atctgccatt ccgatctgtc
     1021 gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat
     1081 tgggcgcgtg gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg
     1141 tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa
     1201 tcagatcaac tgcgagcaag gtgcggtgcc gacgattatg aatcgcggtg gctttgccga
     1261 gaagttgcgt gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc
     1321 cgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat
     1381 cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa
     1441 acttctgcac gcaatgggat gcgaggtgac agcctttagt tctaatccgg cgaaagagca
     1501 ggaagtgctg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa
     1561 agcactggcg gggcagtttg atctcattat caacaccgtc aacgtcagcc tcgactggca
     1621 gccctatttt gaggcgctga cctatggcgg taatttccat acagtcggtg cggttctcac
     1681 gccgttgccg gttccggcct ttacgctgat tgcgggcgat cgcagtgtct ctgggctctg
     1741 ctaccggtac accttatgag ttgcgtaagc tgatgcgctt tgccgcccgc agcaaggttg
     1801 cgccgactac cgaactgttc ccgatgtcga aaattaacga cgccatccag catgtgcgcg
     1861 acggtaaggc gcgttaccgc gtggtgttga aagccgattt ttgaaaatca ttcgcagcgc
     1921 tgatctgagg cgctgccctc tttcgcacat attctgtttt gtcgtatcgc cagcacagcc
     1981 tgccgacatt gttcggtgac attggcaata tcatggttga tatcaatgcg cacaatatcc
     2041 tgttcatctg catgtggacg ctccagtgcc tcaaactgac tttttagtaa cgctaccggc
     2101 ataaaatgcc cagcccgacg ctgcattcgc gcgagaatag tttcatagtc gccatctaac
     2161 cagaggaaat gaacatgggg gctacccttg cgtaaaatat cacgatactg tttttttaat
     2221 gacgaacaga caataaatcc tgtttcattc tttttataaa gactgtatga agcatcattt
     2281 aagcgttcca gccagggaag tcgatcttca tcagataatg gaataccctg cgacatttta
     2341 tctatatttt tggctggatg aagatcgtca ccatcaataa atttagcaga taataacgcg
     2401 gcaaccttgc taccaattaa tgttttacca ctccctgaaa cgcccatcaa aataaagctt
     2461 tcacccgcca tttaaaatct cctgaatcat cttacctgct gcgatactac gcctggcggc
     2521 ataataattt cacgtttttt tatttctgct gataagaatt acaaggcaca tcacgttatg
     2581 cgtaacatag taatgtaaca attttctgac gtgatcttca tcacaaataa tgacaattaa
     2641 aaccgcttaa atgcttccga ggtgtctacc tgaccagtga ggaatttatg caagtgaaaa
     2701 cacagtcctg cgttgttgcg ggcaagaaaa ctgttgccgt taccgagcag acgatagatt
     2761 ggaataataa tggaacatta gtacaaataa cccgaggtgg aatttgcggt tccgatttac
     2821 attattatca ggaaggaaaa gtaggtaatt tcatgataaa ggcaccgatg gtgttaggtc
     2881 atgaagttat cggtaaagtt attcatagcg actcatcaga attacatgaa gggcaaacgg
     2941 tagccattaa tccgtctaaa ccgtgcggtc actgcaaata ctgcattgaa cataacgaga
     3001 atcagtgtac agatatgcgt ttttttggca gtgccatgta tttccctcat gttgatggtg
     3061 gttttacccg ttataaaatg gtcgaaacgt cgcaatgtgt cccttatccg gccaaagctg
     3121 acgaaaaggt tatggctttt gccgaacctt tagccgtcgc gattcatgcc gcacatcagg
     3181 ccggcgagtt acagggcaag cgagtattta tttccggtgt tggacccatt ggctgcctga
     3241 ttgtcagtgc agtgaaaaca ctgggggccg cggaaattgt ctgtgctgat gtgagtcccc
     3301 gttccctttc gctgggcaaa gagatggggg cggatgtgct cgtaaaccca caaaacgacg
     3361 acatggatca ctggaaagcg gaaaaaggct atttcgatgt cagctttgaa gtgtccggtc
     3421 atccttcatc agtgaatacc tgtctggagg tcactcgtgc acgcggcgta atggtgcagg
     3481 taggtatggg aggcgcgatg gcagaattcc caatgatgac gttgattggt aaggagattt
     3541 cactcagagg ctctttccgt tttaccagcg aatttaatac cgcagtgtca tggctggcga
     3601 atggcgttat caatccactg cctttactga gtgctgaata tcccttcact gacctggaag
     3661 aggcgctacg tttcgccggt gataaaaccc aggcagcaaa agtccagctt gttttctaag
     3721 agataaataa aggaataata caatgaacga tctattttca ctggcaggaa aaaatatctt
     3781 gattaccggt tcagcacagg gcattggctt tttactggca accggcctgg gtaaatatgg
     3841 cgcacaaata attattaatg atattactgc cgaacgcgca gaacttgctg tagaaaaact
     3901 ccaccaggag ggtattcagg ccgttgccgc accttttaat gttactcata aacatgaaat
     3961 tgatgccgcc gttgaacata tcgaaaagga catcggcccc attgatgtgc tggtgaataa
     4021 cgccggtatc cagcgccgtc atccttttac tgagttccct gaacaagagt ggaatgatgt
     4081 gatcgcagta aaccagaccg ccgtgttcct ggtatcgcaa gcggtaactc gtcacatggt
     4141 tgaacgcaag gcaggtaaag ttattaatat ttgctcgatg caaagcgaac tgggacgtga
     4201 caccatcacc ccttatgccg catcgaaagg ggcggtaaaa atgctcaccc gcggcatgtg
     4261 cgtcgagctg gcgcgccaca atattcaggt caacggtatt gcgccgggct atttcaaaac
     4321 agaaatgact aaagcactgg ttgaggacga agccttcacc gcctggttgt gcaaacggac
     4381 ccccgccgca cgctggggag atccgcagga actgattggt gctgcggtgt tcctttcttc
     4441 aaaagcctct gatttcgtaa acggccacct gttgtttgtt gatggcggca tgttagtggc
     4501 tgtttaacag ccccccctac gtgcagcgcg aaagcgctgc ttttctggca ctaataagag
     4561 ataaagacta tgccattaat cattattgcg gcaggcgtcg cgctgcttct tatcctgatg
     4621 atcggcttta aagttaacgg ctttattgcc ctcgttctgg tagctgccgt cgtcggattt
     4681 gccgaaggga tggatgcaca ggccgtcctg cactctatac aaaatggtat cggcagcacg
     4741 ctcggcgggc tggcaatgat cctcggtttc ggggccatgt taggcaagct gatttctgat
     4801 acgggtgcgg cacaacgtat cgccactacg ctgattgcta cttttggtaa aaaacgcgtg
     4861 caatgggcgc tagtgatcac cggtctggtt gtgggcctcg ccatgttttt tgaagtgggt
     4921 tttgtcctgc tgttgccgtt ggtatttacc atcgtagcat catcaggatt acccctgttg
     4981 tatgttggcg taccaatggt agcagcgctc tctgtaaccc actgttttct gccgccacat
     5041 ccagggccta ctgccatcgc gactatcttt gaggctaatc tcggaacgac tttactgtat
     5101 ggatttatca ttaccattcc gacagttatt gtcgcaggac cgctgttttc taaactgcta
     5161 actcgctttg agaaagcacc accggaaggc ttatttaatc ctcatctgtt tagcgaagag
     5221 gagatgccct ccttctggaa cagtattttc gctgccgtga tcccggtcat cctgatggct
     5281 atcgccgccg tttgtgaaat tacgttaccg aaaactaaca ccgtgcgcct cttctttgaa
     5341 tttgtcggta accctgccgt tgcgctgttt attgccattg ttattgcgat tttcacactg
     5401 ggccgacgta atggacgcac catcgagcaa atcatggata tcattgggga ttctataggc
     5461 gctatcgcga tgattgtgtt tattatcgct ggcggcggcg cgtttaagca ggtattagta
     5521 gatagcggtg tcgggcagta tatttcacac ttaatgaccg gaactacgct ttcgccgtta
     5581 ttgatgtgct ggactgttgc ggcgctgttg cgtatcgctc tgggctctgc caccgtcgcg
     5641 gccattacca ccgcgggtgt ggtgttgccg ataatcaacg ttacccatgc cgatcccgct
     5701 ttaatggtac tggcaaccgg tgcgggcagt gtgatcgcgt cacacgtaaa cgaccctggc
     5761 ttctggctat ttaaagggta ttttaatctg acggttggtg aaacgttgcg tacctggacg
     5821 gtgatggaaa cccttatttc tattatgggt ttgctgggcg tgttagccat taacgccgta
     5881 ttgcactgaa agaataaaat attgtgatgt agtttcgggg gaagattgaa gattcccccg
     5941 acgttgggag aagttatgcg caatcacaga atttctttac aggatatcgc tacgctggct
     6001 ggcgtaacaa aaatgaccgt gagtcgttat atccgctcgc cgaaaaaggt ggcaaaggaa
     6061 acaggcgagc gcatcgccaa aattatggaa gagatcaatt atatccctaa tcgcgctccc
     6121 ggcatgttgt tgaatgcaca aagttatacc ctcggtatct tgattccgtc atttcaaaat
     6181 caactcttcg ctgacattct cgcgggaata gaatccgtca cctctgagca taattatcag
     6241 acccttattg ctaattacaa ttacgatcgc gattccgaag aagagtcggt gattaacctg
     6301 ctctcttata acattgacgg gattatactt tcggaaaaat atcacaccat caggacggtg
     6361 aaatttctgc gttcagccac cattccggtc gtggaattga tggatgtaca gggagaacgg
     6421 ctggatatgg aggtcggttt tgataatcgg caggccgctt ttgacatggt gtgtaccatg
     6481 ctggagaagc gagtcaggca caaaattctg tatctcggtt ccaaagatga cacccgcgat
     6541 gaacagcgtt atcaggggta ttgcgatgcg atgatgctgc ataatctttc cccattacgc
     6601 atgaatccac gcgccatctc atccattcat ttaggaatgc agttaatgcg tgatgccctt
     6661 agcgcaaacc cggatttaga cggtgtattt tgcaccaatg acgatattgc gatgggtgcg
     6721 cttctgctgt gccgcgagcg aaacctcgcc gtgccagaac aaatttccat tgcgggtttt
     6781 catgggctgg agattggcag gcaaatgatc ccaagcctcg ccagcgtaat tactccccgt
     6841 tttgatattg gtcggacggc tgcccaaatg ttgctgagca aaattaaaaa taacgatcac
     6901 aaccacaaca ctgttgacct cgggtatcag atttatcacg gcaacacgct ttaggttatt
     6961 ttctcttcat tacacgtatt ctctttgccg gacgttgcct atactccagg caggaaactg
     7021 gaggaaatct catgagcgaa cccctgttaa ttgcccgcac gccggacaca gaactatttt
     7081 tactgccggg aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa
     7141 ccgttacgct gcaaaaactg gcagagtcat tgtcggaaat cggcgtgccg gtgtttatgg
     7201 ctgatgtgaa aggcgatctg accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc
     7261 tcgcaaggct taaaaatatc ggcgtcaatg attggcaacc gcatgccaat ccggtggtgg
     7321 tgtgggatat ctttggcgag aaaggccatc cggtgcgggc gacggtttcg gatctggggc
     7381 cgctgttgct ggcgcggctg ttgaatctca acgatgtgca atctggcgtg ctgaatatca
     7441 tcttccgcat tgctgacgat caggggctgt tactgctcga ctttaaagat ctgcgggcaa
     7501 ttacccagta catcggcgat aacgccaaat ccttccagaa tcagtacgga aatatcagta
     7561 gcgcatcggt tggtgccatc cagcgcgggc tgttgtcgct ggaacagcaa ggcgcagcac
     7621 acttctttgg tgagccgatg ctggatatca aagactggat gcgcaccgat gccaacggta
     7681 aaggcgttat caatatcctc agcgccgaga aactttatca gatgccgaaa ctgtacgccg
     7741 ccagcctgct gtggatgctt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg
     7801 agaaaccaaa actggtgttt ttcttcgacg aggcacatct gctgtttaac gatgccccgc
     7861 aggtactgct ggataagatt gagcaggtga taaggcttat tcgctcaaaa ggcgtgggcg
     7921 tctggttcgt ttcgcaaaac ccgtctgata ttccggataa tgtgctcggc cagctcggta
     7981 atcgcgttca gcacgctttg cgggctttta cgccaaaaga tcagaaagca gtgaaggcag
     8041 cggcgcaaac catgcgggcc aatccggcat ttgataccga aaaggcaatc caggaactgg
     8101 ggaccggcga ggcgttaatc tcgtttctcg atgcaaaagg aagtccttct gtggtggaac
     8161 gagcgatggt gattgcgcct tgttcgcgga tgggaccggt gacggaagat gagcgcaatg
     8221 gcttgattaa ccactccccg gtgtatggca aatatgagga tgacgtggac cgggaatccg
     8281 cctatgagat gttgcaaaaa ggctttcagg ccagtaacga gcagcaaaat aatccccccg
     8341 cgaaagggaa agaggtagcg gtagatgacg gcattcttgg tggattgaag gatattttgt
     8401 tcggcactac cggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggccaaaa
     8461 gcgccgctcg ccaggtgacg aatcagattg tacgcgggat gttggggagt ttgctggggg
     8521 ggagaaaaag gtaagccagg tgcgatttgt ctttgcctga tgcgacgctg gcgcgtctta
     8581 tcatgcctac accactgcaa tatattggat tttcataatt tttcaggaat gaacgaagca
     8641 cactttgcta ataataataa agggagcttt cgctcccttt atgcgttcat tagattacga
     8701 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
     8761 aatgatcggc gggatgccgt aaaccaacgt cagcggaccg aagatctggt ccagtacgta
     8821 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
     8881 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
     8941 gattttgctc cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata
     9001 cttcacgtag ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc
     9061 cagtttgtcc ggtgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtaat
     9121 ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc
     9181 cgggtcaaac ttcgcagtag cggcatagcg tacggattgc agacgacgat tctcgttaaa
     9241 ggcataaatg ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtagacgaa
     9301 gttgttgcca tctttcgccc ataacccttg ctgggtagag agcaacgagc cgccgtacat
     9361 cgcctgcgca cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcgccaat
     9421 cgccatcgtc agtaagacca gcggaatggc ggttttcatc accgacagcg ccacctgcat
     9481 acgggtaaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag
     9541 accaagcaac gccccaagca gagccgccat cgggaagaag atctgcacat ctttcggcac
     9601 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     9661 cagctgatcg acaaacttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat
     9721 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     9781 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     9841 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg
     9901 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga
     9961 tacagcagca tggctggcag catcgacagt acgcgtccct gacgtgggtt aaccacgctc
    10021 agcggtacga ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc
    10081 agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg
    10141 gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa
    10201 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
    10261 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
    10321 gaaggacgtg cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg
    10381 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
    10441 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
    10501 cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc
    10561 gcaaggatca ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc
    10621 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
    10681 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
    10741 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
    10801 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
    10861 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
    10921 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
    10981 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
    11041 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
    11101 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
    11161 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
    11221 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
    11281 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc
    11341 gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga
    11401 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat
    11461 ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt
    11521 gcgtcaggct gtcgagacgg caaaagagac gctctacagt ttcgatcagc tgaaaacgaa
    11581 caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
    11641 actgaccagc ggtgagcgcg cgatccagca cggtctggcg attgccgccg ggattaaagc
    11701 agcaaaggat cttggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca
    11761 agcgcgccag ctggctgaca gctacagcaa gaatgttatc acccgcgtta tcggcgaaca
    11821 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcaaaacga
    11881 atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
    11941 gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg
    12001 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggcg tgatgcggat
    12061 ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat
    12121 gcctggcgga cgagcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt
    12181 tgaagtgctg aatactgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt
    12241 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat
    12301 cgcgctgggt catcatatta ctggtctgat ggcgaaccat aatccgctgg cccatgaact
    12361 gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta
    12421 tcaggaacaa ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
    12481 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcacct
    12541 ggatatcgcc ggtaccgcct ggcgttctgg taaagcaaaa ggcgccaccg gtcgtccggt
    12601 agcgttgctg gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaattgcg
    12661 tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct tatccgacct acacagcact
    12721 gaactcgtag gcctgataag acgcgcaagc gtcgcatcag gcaatgctgt tattgtcgga
    12781 tgcggcatga acgccttatc cgacctacac agcaccgaac tcgtaggcct gataagacgc
    12841 ggcagcgtcg catcaggcaa tgctgttatt gtcggatgcg gcgtgagcgc cttatccgac
    12901 ctacacacag cactgaactc gtaggcctga taagacacaa cagcgtcgca tcaggcgctg
    12961 cggtgtatac ctgatgcgta tttaaatcca ccacaagaag ccccatatat gaaaaacgcg
    13021 acgttctacc ttctggacaa tgacatcacc gtcgatggct taaacgccgt tgagcaactg
    13081 gtgtgtgaaa ttgccgcaga acgttggcgc agtggtaagc gcgtactcat cgcctgtgaa
    13141 gatgaaaagc aggcttaccg actggatgaa gccctgtggg cgcgtccggc agaaagcttt
    13201 gttccgcata atttagcggg agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg
    13261 ccgcaaaagc gcagcagcag cccgcgcgat atattgatta gtctgcgaac aagctttgca
    13321 gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga ttctctgaaa
    13381 caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct gaatacggca
    13441 acctggaaat aatggaaaag acatataacc cacaagatat cgaacagccg ctttacgagc
    13501 actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa agtttctgca
    13561 tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa
    13621 ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc ctgtggcagg
    13681 tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag attgccgcag
    13741 aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa atctgggaat
    13801 ggaaagcgga atccggcggc acgattaccc gtcagatgcg ccgtctcggc aactccgtcg
    13861 actgggagcg tgaacgcttc acgatggacg aaggcctgtc caatgcggtg aaagaagttt
    13921 tcgttcgtct gtataaagaa gacctgattt accgtggcaa acgcctggta aactgggacc
    13981 cgaaactgcg caccgctatc tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga
    14041 tgtggcacat ccgctatccg ctggctgacg gtgcgaaaac cgcagacggt aaagattatc
    14101 tggtggtcgc gactacccgt ccggaaaccc tgctgggcga tactggcgta gccgttaacc
    14161 cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctaccg ctggttaacc
    14221 gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga
    14281 aaatcactcc ggcgcacgac tttaacgact atgaagtggg taaacgacac gcgctgccga
    14341 tgatcaacat cctgaccttt gacggcgata tccgtgaaag cgcccaggtg ttcgatacca
    14401 aaggtaacga atctgacgtt tattccagcg aaatcccggc agagttccag aaactcgagc
    14461 gttttgctgc acgtaaagcg gtcgttgctg cggttgacgc actcggcctg ctggaagaaa
    14521 ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtagtt atcgaaccaa
    14581 tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggcg gttgaagcgg
    14641 ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac ttctcctgga
    14701 tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac cgtatcccgg
    14761 catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa gtgcgcaagg
    14821 aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc gacacctggt
    14881 tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaacacc gacgccctgc
    14941 gtcagttcca cccaaccagc gtgatggtat ccggcttcga catcatcttc ttctggattg
    15001 cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc
    15061 cgttccacac cgtttacatg accggcctga ttcgtgatga cgaaggccag aagatgtcta
    15121 aatccaaggg taacgttatt gatccgctgg atatggttga cggtatttcg ctgccagaac
    15181 tgctggaaaa acgtaccggc aatatgatgc agccgcagct ggcggacaaa atccgtaagc
    15241 gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc ctgcgcttca
    15301 ccctggcggc gctggcttct accggtcgtg acatcaactg ggatatgaag cgcctggaag
    15361 gttaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg atgaacacag
    15421 aaggtcagga ttgcggcttc aacggtggtg aaatgacgct gtcgctggcg gaccgctgga
    15481 ttctggcaga gtacaaccag accatcaaag cgtaccgcga agcgctggac agcttccgct
    15541 tcgatatcgc tgcaggcatt ctgtatgagt tcacctggaa ccagttctgc gactggtatc
    15601 tggagctgac caagccggta atgaacggtg gcaccgaagc cgaactgcgc ggtactcgcc
    15661 atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc attccgttca
    15721 tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc gacaccatca
    15781 tgctgcagcc gttcccgcag tatgatgcgt ctcaggttga tgaagcggcg ctggccgaca
    15841 ccgagtggct gaaacaggcg atcgttgccg tacgtaacat ccgtgcagaa atgaatatcg
    15901 cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca gaacgtcgcg
    15961 taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc accgtgctgc
    16021 ctgccgatga caaaggtccg gtttccgtta cgaagatcat cgacggcgca gagctgctga
    16081 tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgtctggcg aaagaagtgg
    16141 cgaagatcga aggcgaaatc agccgtatcg agaacaagct ggcgaatgaa ggctttgtcg
    16201 cccgcgcacc ggaagcggtc atcgcgaaag agcgtgagaa gctggaaggc tatgcagaag
    16261 cgaaagcgaa actgattgaa cagcaggctg ttatcgccgc gctgtaatca aaacacagtg
    16321 atgaaaacga aggccggagc atgctccggc cttttttatc tcttacagaa atggtaaaga
    16381 aggcataatt ccccgtgaaa tactggctaa gaagccttta tctggttgtt cttctttatt
    16441 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
    16501 catccatatt ttcagcagtg gataagccag accgcccgta atcccggata tcaccaccaa
    16561 cgcacacatg cgataaagca taccgtggta tgttaaagtt gagcgaaaac gaatacgccc
    16621 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
    16681 gctaacagca atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc
    16741 cataaattgc tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt
    16801 taatatttga tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa
    16861 tgcgaggatg gcatatttta tacaatatgc agtatctaat ttcaccttga aacgatgtat
    16921 accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa
    16981 aataccaatg gaaacaatac caactattgc catcagaacc acagatataa taacactact
    17041 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
    17101 cattaaaatc ggcaagaaaa aggttaccca ccagaacctt ttcatagagc aattaaaact
    17161 aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat
    17221 aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaacaagcg gcatatctgc
    17281 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac
    17341 attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcatagag
    17401 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
    17461 caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga
    17521 atgggacgga acatccattt cattaataac ttgagccata aaaattccct gaattaaaaa
    17581 atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct
    17641 tatcagtaat aggctggcaa aaactttatt gctttataag atgcttgcgc acgtaagaag
    17701 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
    17761 attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgttattgcc
    17821 cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc
    17881 gccgatccga atcttgacga actgtatcaa gtgtatagtc agccgggtca tgcatattgg
    17941 gtggttgaat acgagggtga agtggtcggc ggcggtggga ttgcgccatt agcaggtagt
    18001 gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg cggtaaaggg
    18061 ctggcgaaaa aactggcctt aatggcgatg gagcaggcac gagaaatggg cttcaaacgc
    18121 tgctatctgg aaacgaccgc ttttttaaag gaagccattg cgctttatga gcatttgggc
    18181 tttgagcata tcgactatgc gcttggctgc acgggccatg tcgattgtga agtgcggatg
    18241 ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt aatcaattaa
    18301 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc catcttcgcc
    18361 gttaggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
    18421 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
    18481 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
    18541 ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca gatcgtctgc
    18601 ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc
    18661 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
    18721 tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc attgcctcca
    18781 cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca tataaattga
    18841 attttaattc attgaggcgt tagccacagg agggatctat gtccgggttt tatcataagc
    18901 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
    18961 cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact ggtaaaaaca
    19021 tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa gttgccgcat
    19081 atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt ggtcataaag
    19141 agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg
    19201 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
    19261 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
    19321 atttacccgg taaagcattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
    19381 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtttggtcg
    19441 cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgct ctggcacagc
    19501 aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggcgttgaa ggtgctgact
    19561 ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agagaaatgg gcagaacgga
    19621 ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg
    19681 aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga ccagacgacg cttggtaaga
    19741 aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat
    19801 ctgccgccag cattgttttt gatcaggcgg aaaaccgtat gcataccatc aaagcagtga
    19861 tggtcgcgac gctcagtaac taattgatat cgctataaag atgccgggaa atacaaaact
    19921 atcccggcat cacattattt tatgcagtga taggtctatt cttccaccca attttttcta
    19981 ttgcagaacg taaatcttgc ggtggattta atttttctaa ctgtttttca ttaatataat
    20041 catatatatt gattttccca tcccgcaggg tttccatcat tcttgttcta atttttaatt
    20101 cattacccat cttttcttca aaaatcttaa cgacataacc cggatatttt tttacaattt
    20161 catttgtttt ttcttcatta ccataaacat atcctaactc attaagatac agctgttcgc
    20221 cctcatactt cttaagtaat tgatagaagt accacttcgc aaaatcaggt tgcttcatga
    20281 aaaaatcctt caaaaaaata tcatttacta aaatgctgac aggcacttca tcaaatagca
    20341 acttaacatc atcaaataac cttagtatta agcgtgattt ttcttgattg cttaagttaa
    20401 aatcattgac atatgcaacc aacttatccc aaatcaattc ctgtaattta cggctatttt
    20461 tatagggttc tttatcgaga ttgaaaaata aggtctcagc tattttaatt ttagattgat
    20521 cgcagctaaa atctttagat tgatcgcggc taaaatcaat atgatttatc acggagcatg
    20581 catactttat cttttcagaa tcaccaaaat attgcattat tttattaaaa caccaatcca
    20641 gtattcttac atcattaata tatggggatt ttaaaaaagt ctccagaatc gaatcgatga
    20701 ccggtgtaag gttaactttt gcatcatcta aggaatcaat aagaatatga gacaggttaa
    20761 ctttgatgtc attaatttca ttgtcagtag ctttcttatt attaagaaga taattattta
    20821 ctgcgttaag gtcatctatt tctttcagta tccctttttc accaagcaag tatgaaatcg
    20881 attcattaca tacataaggc attaatagca gatgttcgtt ccttattgct aataatgcat
    20941 tattcaatgt tgtttctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
    21001 cctgcgagtt atttaactcc tgcgaatcat ttgactcctg agaattattt agctcctgtg
    21061 gattatttaa ctcctgcgaa ttatttaacg cctgcgaatt atttaactcc tgcgaattat
    21121 ttaactcctg cgaattattt aactcctgta tattatttaa cttctgtaga ttatttaact
    21181 tctgtagatt atttaacttc tgatgattat ttaatagacg cgctatttcc ttgttagctt
    21241 cgcgttggta acacgccaga accttgttag taatcaggtg taatgaacta tcatcttttc
    21301 tttcaaaaga cttcccttca taacgaaaac tcaaaaaatc agattgtgga aatataatgg
    21361 ctgtatgtcc ttgacgagtc cccatagtag tagtggaaag tgaaatttct ataatcacct
    21421 tagatgaatc cgctcgctca ccttcaggat tattcataac aaacttcatt atttcactgc
    21481 catcattatt tttgatataa gatgaggact caatatgagc catcatattt ctacccgctt
    21541 cgttggagcg tttgctaaaa tccacataac ccagggtaaa tatttttgcc aaaaactcca
    21601 aaaatgctcc aggagaaaga atattccttc tgctatcctg ccccccggca atacgggcaa
    21661 aatcattcat tcccacacac caggtattta agacttcatt ttttggttta aaattatcgg
    21721 ctgatgtaat gtgttgagaa taattcaaat ctgatatttt gctcatgacg aactccttga
    21781 gttaataaga acgtctaagc attacaaatt ttactcacaa aaaaatatca aaaaacgctc
    21841 ttttcacaaa ctcgccgtaa acctggagtt atttacggcg aaaagtcatc acgccatcag
    21901 catcttcact actgctttgc gaacctgggc tggcgcgccc actgcgcaca gcggtttatg
    21961 cacttccccc ggataaaaca caacaaaatc accttcatta aggataactg ttttctcatc
    22021 aacgccttcc ggcaaaaatg cgatgtcttt atcagctaac cagtcggtat ccggcgtacc
    22081 tgccggttgt gtgctgaagg tcatgccttc ctgacctttt aacacaatct gaatgtcgag
    22141 atagcgggca tggtactccg cacggcgagc ttcgtacggc tcggtcatat cttccgagat
    22201 aagataaaac agacgattgc cttcgatatc gtgcttgccc tttggcgttt ctaccgtaac
    22261 gtgtgctttg atatgctcaa tcgcctggcg taactcctgg ggtagccacg gctgaagatt
    22321 atgaatattt ccgatgatca tcttttccac tccttaatta aaacaacgtt tcatttctag
    22381 cgttatacga aaagatgatc aattccgcta gccttaacgc gtgaattatt gtcttatcac
    22441 aacttatcgg caggcgcgtt cccgcctttc gggtcaaggc ccgcatcact ccttgagaat
    22501 agtttttatc gctccccccg ccagtgcagc tgttgcacag agttgttcga gggagtgccc
    22561 ctctcgtgcc ttcgcagaaa gccctgacat cacggtactc ataaaatcag tcacacattg
    22621 tgcgctttgt ggatgccgcc tggcgatata gtcataaatg gccgtttctg cggcgtgata
    22681 atattgaacg gcaatatcac gcgcttgtgg atcatgacta tgaatgcctt caagaaccat
    22741 acagccagcg cagccgccgt tttggctata tctgcgcgcc gcttctttta atacctcagc
    22801 caggcactcc cctaccggac gatcatcacg aagaatatcg acaagcggaa tagcttccgt
    22861 accgacatat tcattgagta cacggctaaa taacccagcc ttactgccaa aagccgcgta
    22921 gaggctcggg gggttaatac caagataatc agtaacttca gcaacactga cagcatcgaa
    22981 acctttttgg tgaaaaagca cttttgccgc agagactgcc tgctcaggag cgaaacgtct
    23041 gggacgacct ggaacgcgag attgtttttt agtgaccatt acaaaacttg ttgacagaaa
    23101 gttaaaacag ttttgtaatg catgttacat aataaatcaa ggagtcctta tgggcgcttt
    23161 tacaggtaag acagttctca tcctcggtgg tagccgtgga atcggtgccg ctatcgtacg
    23221 tcgtttcgtc accgatgggg ccaatgtacg attcacctat gcggggtcga aagatgccgc
    23281 tgaacgcctg gcacaagaga ctggagcgac agcagtattc acagatagtg ctgacagaga
    23341 cgctgttatt gatgtcgttc gtaagagcgg cgcattggat atcctggtgg taaatgcagg
    23401 tattggcgtc tttggcgatg ccctggaatt aaatgccgac gatattgatc accttttcaa
    23461 aatcaatatt catgctcctt accatgcctc tgttgaagcc gcccggcaga tgcccgaagg
    23521 cgggcgcatc ttaatcatcg gctccgtgaa tggcgatcgt atgcctgttg caggcatggc
    23581 tgcttatgcc gccagtaaat ctgccctaca aggcatggcg cgcgggctgg cccgtgattt
    23641 tggaccgcgt gggatcacca tcaacgtcgt ccagccaggg ccaattgata ccgacgctaa
    23701 tcccgccaac gggccaatgc gcgatatgtt gcatggtttt atggccatca aaagacatgg
    23761 gcaaccggaa gaggtcgctg gtatggtcgc atggttagca gggccagaag ccagttttgt
    23821 taccggcgcg atgcatacca ttgatggcgc gtttggcgca taaccgacta cgctcaatta
    23881 agcccagcca ttatccatga tgtctgggtt ttgtttactc acgtcgtccg ctaaaagcgg
    23941 ctcccggtaa atataaatct tctgttcatg tcgtaaatct tgaggataac caaatggtag
    24001 aaagaaccgc tgttttccct gctggccgac attcactgta tgcagaacat cgttattctg
    24061 cggctattcg ttccggcgat ttgctgtttg tttccgggca agtaggaagt cgagaggacg
    24121 gatcaccaga acccgatttt cagcaacaag tcagactggc atttgataat ttgcatgcga
    24181 ccctggctgc tgcgggatgc acttttgaca atatcattga tattacgagc tttcataccg
    24241 atccagaaaa acaattcgaa gacatcatga cggtgaaaaa tgaaatattt agcgccccac
    24301 cttatccaaa ctggacggcg gtgggtgtta catggctggc aggctttgat tttgaaatta
    24361 aagtgatagc gcgcatccct gagctgtaag caatagtgtt agccgttcgt tttcacactc
    24421 cgccctataa gtcggatgaa tggaataaaa tgcatatctg attgcgtgaa agtgaaaaag
    24481 aaaaaagcag gaaatgtctg caattattga taccgaacga cagttcccct ccagaatcac
    24541 agtaaataaa aatgcatata ttttgacttt aaattcaaat aaaccgtttg cgctgacaaa
    24601 atattgcatc aaatgcttgc accgcttctg acgattagta taatgccgga caatttgccg
    24661 ggaggatgta tggttcagtg tgttcgacat tttgtcttac cgcgtctgaa aaaagacgct
    24721 ggcctgccgt ttttcttccc gttgatcacc cattcccagc ccctcaatcg aggggctttt
    24781 ttttgcccag gcgtcaggag ataaaagatg gctaatccgc tatatcagaa acatatcatt
    24841 tccataaacg accttagtcg cgatgacctt aatctggtgc tggcaacagc ggcaaaactg
    24901 aaagcaaacc cgcaaccaga gctgttgaaa cacaaagtca ttgccagctg cttcttcgaa
    24961 gcctctaccc gtacccgcct ctctttcgaa acttccatgc accgcctggg tgccagcgtg
    25021 gtgggcttct ccgacagcgc caatacatca ctgggtaaaa agggcgaaac gctggccgat
    25081 accatttcgg ttatcagcac ttacgtcgat gcgatagtga tgcgtcatcc gcaggaaggt
    25141 gcggcgcgcc tggccaccga gttttccggc aatgtaccgg tactgaatgc cggtgatggc
    25201 tccaaccaac atccgacgca aaccttgctg gacttattca ctattcagga aacccagggg
    25261 cgtctggaca atctccacgt cgcaatggtt ggtgacctga aatatggccg caccgttcac
    25321 tccctgactc aggcgttagc gaagttcgac ggcaaccgtt tttacttcat cgcgccggac
    25381 gcgctggcaa tgccgcaata cattctggat atgctcgatg aaaaagggat cgcatggagt
    25441 ctgcacagct ctattgaaga agtgatggcg gaagttgaca tcctgtacat gacccgcgtg
    25501 caaaaagagc gtctggaccc gtccgagtac gccaacgtga aagcgcagtt tgttcttcgc
    25561 gccagcgatc tccacaacgc caaagccaat atgaaagtgc tgcatccgct gccgcgtgtt
    25621 gatgagattg cgacggatgt tgataaaacg ccacacgcct ggtacttcca gcaggcaggc
    25681 aatgggattt tcgctcgcca ggcgttactg gcactggttc tgaatcgcga tctggtactg
    25741 taaggggaaa tagagatgac acacgataat aaattgcagg ttgaagctat taaacgcggc
    25801 acggtaattg accatatccc cgcccagatc ggttttaagc tgttgagtct gttcaagctg
    25861 accgaaacgg atcagcgcat taccatcggt ctgaacctgc cttctggcga gatgggccgc
    25921 aaagatctaa tcaaaatcga aaataccttt ttgagtgaag atcaagtaga tcaactggca
    25981 ttgtatgcgc cgcaagccac ggttaaccgt atcgacaact atgaagtggt gggtaaatcg
    26041 cgcccaagtc tgccggagcg catcgacaat gtgctggtct gcccgaacag caactgtatc
    26101 agccatgccg aaccggtttc ttccagcttt gccgtgcgaa aacgcgccaa tgatatcgcg
    26161 ctcaaatgca aatactgtga aaaagagttt tcccataatg tggtgctggc caattaattg
    26221 cggttggtaa taaaagtctg gctccctata atgagccaga ctttttaccg ctgtaataaa
    26281 ggagaaatca tgagcaaaac tatcgcgacg gaaaatgcac cggcagctat cggtccttac
    26341 gtacagggcg ttgatctggg caatatgatc atcacctccg gtcagatccc ggtaaatccg
    26401 aaaacgggcg aagtaccggc agacgtcgct gcacaggcac gtcagtcgct ggataacgta
    26461 aaagcgatcg tcgaagccgc tggcctgaaa gtgggcgaca tcgttaaaac taccgtgttt
    26521 gtaaaagatc tgaacgactt cgcaaccgta aacgccactt acgaagcctt cttcaccgaa
    26581 cacaacgcca ccttcccggc acgttcttgc gttgaagtag cccgtctgcc gaaagacgtg
    26641 aagattgaga tcgaagcgat cgctgttcgt cgctaatctt gatggaaatc cgggctatca
    26701 tgcccggatt aagtctgatg acaaacgcaa aatcgcctga tgcgctacgc ttatcaggcc
    26761 tacgtgattc ctgcaattta ttgaatttgt tggccggata aggcatttac gccgcatccg
    26821 gcatgaacaa aactcacttt gtctgcaatc tgaatccggg tcaccatgcc cagttgattc
    26881 tttattgcca gccgtaacga cggctataga accctttcac caactgggtt aatgtcatat
    26941 accctgccag aatcgcaacc agccacggga aatagcttaa cggcagcgcc tgcagctgca
    27001 gataactggc cagcggtgaa aacggcaatg cgataccgac aatcatcacg atcacggtca
    27061 tgatcattaa cggccacgat gcacagctct gaataaacgg cacacggcgg gtgcggatca
    27121 tatgcacaat cagcgtttgc gacagtaagc ccaccacaaa ccatcccgac tggaacagcg
    27181 tttgcgtttc cggcgtgttg gcatggaata cccaccacat caggcaaaac gtcagaatat
    27241 cgaagatcga gctgatcggt ccgaagaaga tcataaagcg ccccagatcc gccggattcc
    27301 aacgctgcgg cttttgaatt tgctcgtcgt cgacattatc aaacgggatc gccacctgtg
    27361 acacatcgta cagcaggttc tgaataagca agtgtaacgg caacatcggc aggaagggca
    27421 agaaagcact cgccaccagc acgctgaaca cattaccgaa gttagaactc gccgtcattt
    27481 tgatgtattt cagcatgttg gcgaaagtgc gacgtccctc aataaccccc tcttccagca
    27541 ccatcaggct tttttccagc aggatgatat cagccgcttc acgggcaata tctaccgcgc
    27601 cgtccacaga aatgccgata tccgccgcgc gtaaggcggg cgcatcatta ataccatcgc
    27661 ccataaagcc aaccacatgc ccttcgcgct tcagtaaggt cacaatgcgt tctttatgca
    27721 tcggcgtcag gcgggcaaac agcgtggtgc gctgtgcaag atttgccagt tcgtcgtcag
    27781 atagcgtttc aatatcacta ccaatgacca cctctcccgc gtccagcccc acttcatggc
    27841 acactttcgc tgcaactaac tcactgtcgc cagtaaggat ttttacggta attccactcg
    27901 cttttaatgc cttcagtgcc ggagcggttg tctcttttgg cggatcaaga aaagcaatat
    27961 atccttcaag gatcaggtcg gattcatccg cccgctggta atctccttca cgcgctggca
    28021 ggtatttcgt cgccaccgca accacgcgca gcccctgacg attcagcgta tcagtaaccc
    28081 gcttaatctt acgcagcatg atgtcatcga gcggcacaat ctcgccattg tgacgcacct
    28141 gcgaacacac attgaggatt tcctgcaatg cgcctttgca aaccagctga tggtgctcgg
    28201 tattttctgc cactaccacc gacatccggc gacgctcgaa atcaaacgga atctcatcga
    28261 ttttttgcca acgactggcc agcgagcgcg ctgactcttc atccgtacct tcgagcaccg
    28321 ctgtatcgag caggttttta agtccggtct gataatgact gttcaaccac gcgctatgca
    28381 gcacgcgttc gctggtttta ccggagatat cggtatgatt ctccagcaca attttatcct
    28441 gcgtcagggt gccggtttta tcagtgcaca gaatatccat tgcgccaaag ttctgaatag
    28501 catccagatg tttgacgatc actttctgtt tcgacagctt tactgcgccg cgcgccagcg
    28561 tcgaggtaac aatcatcggc aacatttccg gcgttaggcc taccgcgacc gaaagcgcaa
    28621 acagcgccgc ttcccaccag tcgcctttgg tgtagccatt gattaacagc accaccggtg
    28681 ccatcaccag cataaagcga atcagcagca tactgacgcg gctgatccct tgctgaaagg
    28741 cattcggctc gctttcctgc tcactaacac gccccgccag ttgaccaaac caggtattgg
    28801 cacctgtagc aatcaccatt gcttgtgccg tgccgctcac cacggtggtg cccataaaac
    28861 acagcgtgtc gcactccagc ggattgctgt gctccggctg gcgagtggta gcggcttttt
    28921 ctacgggcag agactcaccg gttaacgacg cctgagcgac gaacagatcc cgcgcctgca
    28981 agatacgtaa atctgccggg atcatatctc ccgccgccag ttttataata tcgccgggca
    29041 ccagctggtc gatcgggatc tccagccagc cattttcgcc tttgtcgtta attacgcgca
    29101 gcaccgtcgc agtattgctg accatcgctt tcagggcatc tgccgcttta gtggaacgtg
    29161 cttcctgaat aaagttcagc aacgtagaaa tagcgaccat tagcgcgata acgcccgcag
    29221 caaataaatc ttccgtggcg taagaaatag cgccgagaat ggtgagtaaa atattaaagg
    29281 ggttgcgata gcagacccat aaatgtaccc accacggcga cggttgttgt gcgggtaatt
    29341 tattttcacc atgttgttcg cgggcagatt ccacttccgc ctgatttaac ccttccggat
    29401 gggtgtcgaa cgttttccac aattcttctt cgggcatcac cgccattttc aggcaatgcg
    29461 cacttaagga cggcgggacc accgtattca ctgtctgctg cgcgccaggc aatggatcac
    29521 gatgaaccag acgggaaggt aaatggcgaa tgagccgggt aaaaatttct ttaaacataa
    29581 ggagtccctc cgcactgtct gaaacagtac agaaatgtct tcaggcgtgg cgaagcctta
    29641 cctgaaggta ataaagctga gcagggacgt taaaacgtca cggccttacg catccggtaa
    29701 cgccgtgaaa agacgggctt accggaaaag ttttaatctc cgtcgaggga gaggcgtggg
    29761 atcaggttcc atataacctc cggtaagtga aaaaagcgcc gcaaataata aggagaatcc
    29821 aataatattc gtagcatcta tggcgatata atacctgctg gcaactaaac caacgataaa
    29881 ccagacttta cccattgctg aatgcacggg taaagttagg ctcaaataat taaacaacac
    29941 gttacaggac aacaggatgc aaaatcggct gaccatcaaa gacatcgcgc gcttaagcgg
    30001 cgtggggaaa tctacagttt cccgggtgct gaataacgaa agcggcgtga gccagcgcac
    30061 ccgcgagcgt gttgaagcag tgatgaatca gcatggattt tccccttccc gctctgcgcg
    30121 cgctatgcgt gggcaaagcg ataaagtggt cgccatcatt gttacccgtc tggattcgtt
    30181 gtcagaaaat ctcgccgttc aaaccatgct gccagcgttc tatgaacaag gttacgaccc
    30241 aatcatgatg gaaagtcagt tttccccgca attagttgcc gaacatttgg gggtgctgaa
    30301 acggcgtaat atcgacggcg tagtgctgtt cggttttact ggcataacag aagaaatgtt
    30361 agcccactgg cagtcatcgc tggttctgct ggcgcgtgac gcaaaaggct ttgcttcggt
    30421 ctgttatgac gacgaagggg caatcaagat cctgatgcaa cggctgtatg accaggggca
    30481 tcgtaatatc agttatctcg gcgtgccgca cagtgacgtg acgaccggta agcgacgtca
    30541 cgaagcctac ctggcgttct gcaaagcgca taaactgcat cccgttgccg ctctgccagg
    30601 ccttgctatg aagcaaggct atgagaacgt agcaaaagtg attacgcctg aaactaccgc
    30661 cttactgtgc gcaaccgaca cgctggcact tggcgcaagt aaatacctgc aagagcaacg
    30721 catcgacacc ttgcaactgg cgagcgtcgg taatacgccg ttaatgaaat tcctccatcc
    30781 ggagatcgtc accgtagatc ccggttacgc cgaagctgga cgccaggcgg cctgccagtt
    30841 gatcgcacag gtaaccggac gcagcgaacc gcaacaaatc atcatccccg ccaccctgtc
    30901 ctgatcgttt cctgaacgat aaattgtgat cttcgctgcg tttcgggaac gttcccgttt
    30961 ttaaattttt ccgcgcaata tattctgcag ccaaccaaaa atgtcatctg ccatggggct
    31021 ttatgatgag caaaataaac caaacggata tcgatcggtt gattgaactg gtcggcgggc
    31081 gcggcaatat tgcgacggtg agccactgta ttactcgcct acgctttgtc ctcaaccaac
    31141 cggccaatgc cagaccgaaa gaaattgagc aactccccat ggtgaaaggc tgtttcacca
    31201 atgccgggca atttcaggtg gtgattggca ccaacgtggg tgattactat caagcactga
    31261 tagcgtcaac cggacaggcg caggttgata aagagcaggt aaaaaaagcc gcccggcaga
    31321 atatgaaatg gcatgagcag ttgatctctc atttcgcaga gatcttcttc ccgttgctgc
    31381 ccgcgttgat tagcggcggt ttgatcctcg gttttcgcaa tgtgatcggc gatttgccca
    31441 tgagcaacgg tcaaacgctg gcgcaaatgt acccttccct gcaaacgatc tacgattttc
    31501 tgtggttgat cggtgaagcg atcttcttct acctgccggt cgggatttgc tggtcagcgg
    31561 tgaaaaaaat gggcggcacg ccgatccttg gtatcgtgct tggcgtgaca ctggtttccc
    31621 cccagctgat gaacgcttat ctgctcgggc agcagctgcc ggaagtgtgg gactttggca
    31681 tgttcagcat cgccaaagtg ggctatcagg cgcaggtgat cccggcacta ttagccgggc
    31741 tggcgctggg cgttattgaa actcgcctta aacgcatcgt gccagattac ctctatctgg
    31801 tggtggtgcc cgtctgttcg ctgatcctcg cggtgttcct cgcccatgcg ctgattggtc
    31861 cgtttggtcg catgattggc gatggcgttg cctttgcggt acgtcacctg atgaccggca
    31921 gctttgctcc gattggcgcg gcattgtttg gcttcctgta cgccccgctg gtgatcaccg
    31981 gggtacacca gaccacgctt gctattgatt tgcagatgat tcaaagcatg ggtggtacgc
    32041 cagtgtggcc gctgattgcg ctgtcgaata tcgctcaggg ctccgccgtg ataggcatta
    32101 tcatttccag ccgcaaacac aatgaacgcg agatctccgt gcctgcggcg atctccgcct
    32161 ggcttggggt cactgagcct gcaatgtacg gcatcaacct gaaatatcgc ttcccgatgc
    32221 tgtgcgcgat gattggttct ggtctggcag gattgctatg cggcctgaac ggcgttatgg
    32281 cgaatggcat cggcgtaggc ggcctgccgg gaattctctc gattcaaccg agctactggc
    32341 aggtgtttgc gctggcaatg gctatcgcca tcatcatccc gattgtactc acctcgttta
    32401 tctatcagcg gaaataccgc ctgggcacgc tggacattgt ttaattttct tcggggcgca
    32461 attgcgcccc ctcgcatttg caggaataac gtaatgacta atcttcccca ctggtggcaa
    32521 aacggcgtta tctaccagat ttatccaaag agttttcagg acaccacggg tagcggcacc
    32581 ggcgatttac gtggcgttat ccaacgcctg gactatctgc ataaactggg cgttgatgcc
    32641 atctggctaa ccccctttta tgtctctccc caggtcgata acggttacga cgtagcgaac
    32701 tatacggcga ttgatcccac ctacggcacg ctggacgatt ttgacgaact ggtgacgcag
    32761 gcaaaatcgc gcgggattcg tatcattctc gatatggtgt ttaaccatac ctctacccaa
    32821 catgcctggt ttcgcgaggc gctgaacaaa gaaagccctt accgccagtt ttatatctgg
    32881 cgcgatggag aaccagaaac gccaccgaac aactggcgtt caaaatttgg cggtagtgcg
    32941 tggcgctggc atgcggaaag cgaacagtac tatttgcatc tctttgcacc agaacaggcg
    33001 gatctcaact gggagaatcc agcggtacgc gcagagctga aaaaagtctg tgagttctgg
    33061 gccgatcgtg gggtcgacgg gttgcgcctg gatgtggtga atctgatctc caaagacccg
    33121 cgtttccctg atgacctgga tggcgacggg cgtcgcttct acaccgacgg gccacgagca
    33181 cacgagtttt tgcacgagat gaaccgcgat gtgtttacgc cacgcgggtt aatgaccgta
    33241 ggtgaaatgt cctccaccag ccttgagcat tgccagcgat acgcggcact gactggcagt
    33301 gaattgtcga tgacctttaa ttttcatcac ctgaaggtcg attatcccgg tggtgaaaaa
    33361 tggacgctgg ctaaacctga ctttgtggcg ttgaaaacat tgttccgcca ctggcaacaa
    33421 ggaatgcaca acgtagcatg gaatgccttg ttctggtgta accacgatca gccgcgcatt
    33481 gtttctcgct ttggtgatga aggtgaatac cgcgtgcctg cggcaaaaat gctggcgatg
    33541 gtgctgcatg gcatgcaggg aacgccgtat atctaccagg gcgaagagat tggcatgacc
    33601 aacccgcatt tcacgcgcat tactgactat cgcgacgtag agagcctcaa tatgtttgcc
    33661 gagctgcgca acgatgggcg tgatgccgac gagttattgg caatcctcgc cagtaaatcc
    33721 cgtgacaaca gccgcacgcc catgcaatgg agcaacggcg ataatgccgg gtttacggct
    33781 ggcgaaccgt ggattggcct gggcgataac tatcaacaaa tcaacgtaga agccgcgctg
    33841 gccgatgagt cctcggtgtt ttacacctac caaaagttaa tcgcactgcg taagcaggaa
    33901 gccgttctga catggggcaa ttaccaggat ctgctgccaa acagccctgt attgtggtgc
    33961 tatcgccgtg aatggaaggg gcaaaccttg ctggtcattg ccaaccttag ccgtgagatc
    34021 caaccctggc agccagagca aatgcgcggc aactggcagc ttgtgatgca taactacgaa
    34081 gaagcctcac cacaaccctg tgccatgact ttacggcctt ttgaggctgt ctggtggtta
    34141 cagaagtaaa tcttccctaa gccccggtaa tgccggtcat tccggggttt tgtctgtcag
    34201 tatgttgttt ttgttgattt tttaaccagc aaattcatta aaaaatttac ataacgctgt
    34261 agcgcccgtc atccgtacgc tctgcttttt actttgagct acatcaaaaa aagttcaaac
    34321 atccttgatg caaagcacta tatatagact ttaaaatgcg tcccaaccca atatgttgta
    34381 ttaatcgact ataattgcta ctacagctcc ccacgaaaaa ggtgcggcgt tgtggataag
    34441 cggatggcga ttgcggaaag caccggaaaa cgaaacgaaa aaaccggaaa acgcctttcc
    34501 caatttctgt ggataacctg ttcttaaaaa tatggagcga tcatgacacc gcatgtgatg
    34561 aaacgagacg gctgcaaagt gccgtttaaa tcagagcgca tcaaagaagc gattctgcgt
    34621 gcagctaaag cagcggaagt cgatgatgcc gattattgcg ccactgttgc tgcagttgtc
    34681 agcgagcaga tgcagggccg caaccaggtg gatatcaatg agatccagac cgctgttgaa
    34741 aaccagctga tgtcaggtcc atacaaacaa ctggctcgtg cttacatcga gtaccgtcac
    34801 gatcgcgaca ttgaacgtga aaaacgcggt cgcctgaatc aggagatccg tggtctggtc
    34861 gagcagacca acgcctcgtt actcaacgaa aacgccaaca aagacagcaa ggtgattcca
    34921 acccagcgcg acctgctggc cgggatcgtg gctaaacact atgcacgtca gcacctgctg
    34981 ccgcgtgacg tggtgcaggc acatgagcgt ggcgatattc actatcacga tctcgattac
    35041 tcgccgttct tcccgatgtt caactgcatg ttgatcgacc tgaaaggcat gctgacccag
    35101 ggctttaaga tggggaacgc cgagattgaa ccgccgaagt cgatctctac ggcaaccgct
    35161 gtaactgcgc agattattgc tcaggtcgcc agccatattt atggcggcac caccattaac
    35221 cgtatcgatg aagtgctggc accgtttgtc accgccagct acaacaaaca tcgcaagacc
    35281 gcagaagagt ggaacatccc ggacgccgaa ggctacgcta actctcgtac cattaaagag
    35341 tgctacgatg ccttccagtc gctggagtac gaagtaaaca ccctgcacac cgccaacggt
    35401 cagacgccgt ttgtaacctt tggttttggt ctgggaacca gctgggagtc gcgcctgatt
    35461 caggaatcta tcctgcgtaa ccgtatcgca ggcctcggta aaaaccgtaa aactgcggtg
    35521 ttcccgaaac tggtgtttgc gattcgcgat ggcctgaacc ataaaaaagg cgatccgaac
    35581 tacgacatca aacagctggc gctggagtgc gcaagcaagc gcatgtatcc ggatatcctg
    35641 aactacgatc aggtagtgaa agtcaccggt tcgtttaaaa ccccgatggg ctgccgcagc
    35701 ttcctcggcg tgtgggaaaa tgaaaacggc gagcagatcc acgatggtcg taacaacctc
    35761 ggcgtgatca gcctgaacct gccgcgtatt gctctggaag caaaaggcga tgaagccacc
    35821 ttctggaagc tgctggatga acgtctggtg ctggcacgta aggcgctgat gacccgtatc
    35881 gctcgtctcg aaggcgtgaa agcgcgcgtg gccccgatcc tctatatgga aggtgcttgt
    35941 ggcgtgcgtc tgaatgctga cgatgatgtt tctgaaatct tcaaaaacgg tcgtgcgtct
    36001 atttcgctgg gttacatcgg catccacgaa accattaacg cgctgttcgg cggcgagcat
    36061 gtttacgaca acgagcagct tcgcgcgaaa ggtatcgcca ttgttgaacg tctgcgtcag
    36121 gcagtggatc agtggaaaga agaaacgggt tatggtttca gtctctacag cacgccgagt
    36181 gaaaacctgt gcgaccgctt ctgccgtctc gatactgctg agtttggcgt ggtgccgggc
    36241 gtgaccgata aaggttacta caccaacagt ttccacctcg atgtggagaa gaaggtgaac
    36301 ccgtacgaca agatcgactt tgaagcgcct tacccgccgc tggcgaacgg tggtttcatt
    36361 tgctacggcg agtatccaaa cattcagcac aacctgaagg cgctggaaga tgtctgggat
    36421 tacagctatc agcatgtacc gtattacggc accaatacac cgattgatga gtgctacgag
    36481 tgtggcttta ccggtgagtt cgagtgcacc agcaaaggct tcacttgccc gaaatgtggt
    36541 aaccatgacg cctcccgtgt gtcggtaact cgccgcgtgt gcggatattt aggtagcccg
    36601 gatgcacgtc cgtttaacgc tggtaagcag gaagaagtta agcgccgcgt taaacatttg
    36661 ggaaatgggc agataggttg atctgcacta agcaaatgcc ggatgtgatg tgaaagcgtc
    36721 gcatcccgcg ttgattgccg gatgcggcgt aaacgcctta tccggcctac atttggcaac
    36781 ggctgtaggc ctgataagac gcgacagcgt cgcatcaggc gttgattgcc tgatacagcc
    36841 tacacgcgga ctgataagcc aggcgaaata atgaattatc atcagtacta tcctgtcgac
    36901 atcgtcaacg gtcccggcac tcgctgcacc ctgtttgtct ccgggtgtgt tcatgaatgc
    36961 cccggttgct ataacaaaag cacatggcgg gtaaattccg gtcagccatt tactaaagca
    37021 atggaagacc agatcattaa cgatctgaat gacactcgta tcaaacgcca gggtatttcc
    37081 ctctccggcg gcgatccgct gcatccgcaa aacgtgccgg atattctgaa actggtacaa
    37141 cgcatccgcg ccgagtgtcc gggtaaagac atctgggtgt ggacaggcta taaactcgac
    37201 gaactcaacg ctgcgcaaat gcaggttgtt gatttgatta acgtgctggt cgacggcaaa
    37261 tttgtgcagg atttaaaaga cccttccctg atctggcgcg gcagcagcaa tcaggtggtg
    37321 catcatttgc gatgataagt tccctgttac ctgagtgcag gcaacaggga aataaggaat
    37381 taacgatact tctggtgata ggcgttgcgg gtcgttttca gttgctctgc ggcagcctgc
    37441 gcttctttta ctttaccttc atttgccagc ttcagcgcgt cgtcaatctg accgaccaga
    37501 atgtcgaaac cgtggcggaa atctttcatt tccgggctgt ccggtgattt atcttcgagc
    37561 ttcggcggcg ttgctttttg cgcatccagc gctgcggcgc gcatcttcgt taacgcgtct
    37621 ttgacttgcg ccgcgttatc ggctttttcg accactttta aattgtcgtt gagggtttcc
    37681 atattgtctt caagatcagc ggcaaacgac gccgaactga ataccaacga ggagactgca
    37741 agaatagcta acaggctttt tcgcattgct cacttccttt tttatttaca cttcatcctt
    37801 cacactgcct catcgacgac tacgctcatt cgaccggatc acgtactgcg agtacgctcc
    37861 ccggccctca ttcactttcc gtctcgatgc agtgcgaatg attttgtgta attttttagt
    37921 ttatagcgcg gcaggtcgcg ccagtttttt tactgtccgg cgattttcat ctccggcaac
    37981 agcacagaac cacactgtat attactgcgt gtttcaatat cgttaccgac ggtgacaata
    38041 ttgcgccaca tatcttttaa attacctgcg atggtgattt cgctaaccgg atactgaatt
    38101 tcgccgtttt ctacccagaa gcccgccgca ccacgggaat aatcaccggt aattgcactc
    38161 acgccctggc ccatcaattc cgtcaccacc agcccggtgc ccatctcttt gagcatctgc
    38221 tcgaagctta gaccttgtcc ggcaatccgc cagttgtgaa taccgcccgc atgtccggtg
    38281 cttttcagcc ccagttttcg cgccgaatag ctggtcagca gccactgagt caggatgcca
    38341 tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc tgtcgaatgg cgtcgacgcc
    38401 agccctttca gcagatgcgg atgctcttca atggtcagcc agtccggcag aatttgttta
    38461 cccagcgaat ccagcaggaa ggtagattta cggtaaaccg atccacccgc tatcgccccc
    38521 accagatggc caaaaagccc ggttgccact tcattggcaa aaatgaccgg cgctttcatg
    38581 gtggagagtt tacgcggtga caggcgcgat aaagtacggc gagcacagtc ggccccaacc
    38641 cactctggcg tttgcagatc gctcatcgca cgaccaatgg tgtaggcgta atcacgctcc
    38701 atatcgccat tttcttcggc aattacacag ctggagagcg aatgacgcgt tgagcagtaa
    38761 ccctgcaaca tgccgtggct gttgccaaaa actttgacac cgtagtggct gttaaagctg
    38821 ccaccttcgg tattggtgat gcgtttgtcc gcctgcaatg ccgcctgttc tgcgcgggcc
    38881 gccagttcaa tggcttcatc cggggaaact tccgcagggt ggaacaagtc gagatctggt
    38941 gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac agggatctgg cgaggtataa
    39001 cgggcaatat ccagcgccgc ctgtacagta cgggcaatgg cctgcgggct taaatcggtg
    39061 gatgatgcgc tacctttgcg gttctggtga taaacagtga tccccagcgc gccatcgcta
    39121 ttgaattcaa cattctccac ttcaccataa cgcgtgctta cgctaatgcc ggtggtcttg
    39181 ctgacggcaa cttccgcacc gtccgatttg cctgaggcca actccagcgc agtggaaact
    39241 gcttcttcca gaatcttgcg ctgcgcttca acttgagaga ttactttcat tgcaagtgcc
    39301 ataatgtaga gagtttctct gaagtctaac agagaaccgt ttttcagtgc gcatcttaac
    39361 tggtaacatt agcctctttt tttaaggagc ctgagatgac taagcagccc gaagactggc
    39421 tcgacgacgt tcccggtgat gacatcgaag acgaagacga tgaaattatc tgggtcagta
    39481 aaagtgaaat taaacgtgat gccgaggagc taaaacgcct tggcgcggaa attgttgatc
    39541 tggggaaaaa cgcgctggat aagatcccgt tagatgcgga tctgcgcgcg gctattgagc
    39601 tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt gcagctcatc ggtaagatgc
    39661 tgcgccagcg cgacgtagag cctattcgtc aggcgctgga taagctgaaa aaccgtcaca
    39721 accagcaggt ggtgctgttc cataaactgg aaaacctgcg cgatcgtctg atcgatcaag
    39781 gtgatgacgc catcgctgag gtattaaatc tgtggcccga tgccgatcgt cagcaactgc
    39841 gtactctgat ccgcaacgcg aagaaagaga aagaagggaa taagccgccg aaatccgcac
    39901 gccagatttt ccagtatctg cgcgaactgg cggagaatga agggtaattt tcgttgttga
    39961 tgtgagtatg gtttgccgga tgcggtgcac ctgtaggcct gataagacgc atcaagcgtc
    40021 gcatcaggca ctatgctcaa atgccggatg cggcgtgaac gccttatccg gcctacgcac
    40081 actatatcag gctaaggccg aattactgcg cggcttccgc tttcttcgcc agaccatcca
    40141 gcagtttctg atggatccca ccaaaaccgc cgttgctcat caccagaata tggtcgccag
    40201 gctgagcggt tttcaccacc atatctgcca gcgtatccac atcgccactc cagtgtgcag
    40261 gctgaacgca ggcttctgcc acttctgcca cctgccacgg aatatgcgcc ggttgcagca
    40321 ggaagacttc atcggcacga cctaaagaag gtgccaaatc gtctttgcag atccccattt
    40381 tcatggtatt cgagcgcggt tccagcacag caataatgcg cgccgtacca ccaactttgc
    40441 cacgcagcgc cgccagcgtt gccagaatcg ccgtcgggtg atgggcaaaa tcgtcatata
    40501 ccgtgacgcc attcgcttca ccacgcaact ccagacggcg acgagcatta ataaacgaac
    40561 ccagcgcgtt agcggcatct gccggcgcta caccaacatg gcgagccgct gcaatcgcca
    40621 tcaggccatt gtgcatatta tgttcgccta ccagcgacca tttcacttcg cccacttttt
    40681 cgccatccag caaaacttcc cattcggaag catcggtggt cagctttttc gcctgccagt
    40741 gaccctgctc acccaccagc tcctgctcgc tccagcagcc catcgccatg gtctgtttca
    40801 ggttgatgtc gttttctggc cagataatac ggccctgccc cggaacgata cgcaccagat
    40861 ggtggaactg tttctggatc gctttcaggt cgtcaaagat atcggcgtga tcgaactcaa
    40921 ggttgttgag gatcagcgta cgcgggcagt aatggacaaa tttagagcgt ttgtcgaaga
    40981 aggcgcagtc gtactcatcc gcttcgataa caaagaagtc gctttcgccc agacgcgccg
    41041 aaacctcaaa gttccccggc acaccgccga ttacaaagcc cggtttgtaa ccacactgtt
    41101 ccagaatcca ggtcgccatt cccgcggtgg tggttttgcc gtgtgtaccg gcaacggcca
    41161 gcacccagcg gtcgcgcagc acaaaatcgt gcagccactg tggacctgac atataaggga
    41221 tgtttttttc cagtaccgct tccacacacg gatttccacg ggtcatggcg ttgccaataa
    41281 tcaccagatc cggctgcgga tcgagctggc tggcatcgta accctgaatc agttcaatgc
    41341 cttgcttctc aagtaaggtg ctcatcggcg gatacacatt ggcgtccgaa cccgttactt
    41401 catggcctaa ctggcgcgcc agcatcgcca gaccgcccat aaacgtgcca caaattccta
    41461 aaatatgaat gcgcatacgt cactatcctt ttttaatctg ccgctcattt tacgcatatg
    41521 tcgggcaagt gagaaacgca tttcaggata atccgcaatt tgctggcgcg attcacctga
    41581 gcgcaacatt gagattattt gttaagattg ttgcggtcgc tttactccat aaacattgca
    41641 gggaaagttt tatgaaaacg ttaggtgaat ttattgtcga aaagcagcac gagttttctc
    41701 atgctaccgg tgagctcact gctttgctgt cggcaataaa actgggcgcc aagattatcc
    41761 atcgtgatat caacaaagca ggactggttg atatcctggg tgccagcggt gctgagaacg
    41821 tgcagggcga ggttcaacag aaactcgact tgttcgctaa tgaaaaactg aaagccgcac
    41881 tgaaagcacg cgatatcgtt gcgggcattg cctctgaaga agaagatgag attgtcgtct
    41941 ttgaaggctg tgaacacgca aaatacgtgg tgctgatgga ccccctggat ggctcgtcca
    42001 acatcgatgt taacgtctct gtcggtacca ttttctccat ctaccgccgc gttacgcctg
    42061 ttggcacgcc ggtaacggaa gaagatttcc tccagcctgg taacaaacag gttgcggcag
    42121 gttacgtggt atacggctcc tctaccatgc tggtttacac caccggatgc ggtgttcacg
    42181 cctttactta cgatccttcg ctcggcgttt tctgcctgtg ccaggaacgg atgcgcttcc
    42241 cggagaaagg caaaacctac tccatcaacg aaggaaacta cattaagttt ccgaacgggg
    42301 tgaagaagta cattaaattc tgccaggaag aagataaatc caccaaccgc ccgtatacct
    42361 cacgttatat cggctcactg gttgcggatt tccaccgtaa cctactgaaa ggcggtattt
    42421 atctctaccc aagcaccgcc agccacccgg acggcaaact gcgtttgctg tatgagtgca
    42481 acccgatggc attcctggcg gaacaagcgg gcggtaaagc gagcgatggc aaagagcgta
    42541 ttctggatat tatcccggaa accctgcacc agcgccgttc gttctttgtc ggcaacgacc
    42601 atatggttga agatgtcgaa cgctttatcc gtgagttccc ggacgcgtaa tctcctgcgc
    42661 ctctccacac ggagaggcgt ttttattgct gcgcaatgtt gactcttgtc actggcgaac
    42721 tctgacgatt ctcccacagc accgttaacc cacgctgtaa tgctataaaa ataaacaaca
    42781 aaataccgat ggcgattttc gtccaccatg agctcagcgt gccatcaaag tttatgtaag
    42841 tctgaatcag tccctgaatc gccacgccaa aaagcgtacc gccaatcacc actgaggcga
    42901 tagcgtccag ttccacacct acgcccgcca gcgcatatcc gacctgggta taaatcgaga
    42961 agacaatccc cgccagcgtt gccagtccgg tggagagcat ataaatgcga atagtagtgc
    43021 tacgggtaga aatccccatc aggtttgccg acgtcgcatt gccgccaatg gcgtatacct
    43081 gattaccaaa acgggtacga tgcgcgagga atatgccgat aaccaccacc gccagcatca
    43141 gcagccccat cgcacttaag cgaccgccgc cagggatttt ccatacaagg cttgagagcg
    43201 tgtcataaac aggatggttt atcgggatcg actcttctga gatgagatag ctgacaccgc
    43261 gcaaaaagaa catccctgca agggtaatga taaatgccgg gatcttcagg gcgtcgatca
    43321 aaagccccat aaatgcgccg aaggcacagc ccatcaccag caccagcgga aacgccagca
    43381 gcggtgagag accgaaatct ccaatcactt ttgccagaaa cacgccggta aaggcgatca
    43441 ccgaaccgac ggagagatcg atcccaccgg agaggatcac aaaggtcatg ccaacggcaa
    43501 tgatcccaag aaaggcgtta tcggtcagga tattgcagat cactcttgtg gaagcaaaac
    43561 cgggaaactg ggtcaggcag taaagataac ccaacacaaa gacgccgatg gtgatcatca
    43621 gtggcaaatt acgttttatc acggctacgt actcctttaa tcagactgat aaagcgttgc
    43681 gactggacaa tcagcacgca aagaaccacc accgctttta caacctggtt catctctggc
    43741 ggaaagcccg aaagcaaaat tccggtgttc atcccctgaa taatcagcgc ccccaccacc
    43801 gaaagtagca ggttaaagcg cccgcccatc agcgatccgc cgccaatcac caccgcgaga
    43861 atggcgtcca gctccagcca taacccggcg ttattggcat cggcaccgcg aatatccgcc
    43921 gccacgataa tgcccgcaat cgccgcacac agcccgctca acacgtaggt aagcatgacg
    43981 atgatccgcg tgtttacccc ggcatttttt gccgcccgaa tgttgatacc aacggcttcg
    44041 ataaacatcc ccagcgccgt tttgcgggtc aacagccaga acaggataag cgtcagcacc
    44101 gcgataatga ccggcgttgg caggaacaac agcgatccac tgccgaacca tgagagatcc
    44161 ggcgagttaa acgtgacgat ctgcccggcg gtgatcagtt gcgccacgcc gcgcccggcg
    44221 accatcagga tcagagtggc gacaaacggc tgaattttga ggatcgctac cagtatgccg
    44281 ttccacaatc ccgccaggat gccggtgccc agggcgctta acaaaacaat cggcaggctg
    44341 aatcccgcga ccgtcatcgc agccgttgtg gctccggcga tagccatcac cgcccctacg
    44401 gagagatcaa tcccaccggt ggcgatcacc agcgtcatgc caatcgccag taacgcaacg
    44461 ggagctgcac ggttaagaat gtctatgggg ctaccgaaca aacgcccatc ctgaagcacc
    44521 acctgccaga aatgcggggc taccaggcta tcgaccagca gcaccagcaa tagtgctacc
    44581 agctgcggca tccccgtcgg ccagcgaaag cgcctttttg gcgtagtggt gtccggaaga
    44641 gattgaggca tcacactgtt ctccttacgc cgcaatggcg ttcatgatcg ccggaacgga
    44701 aagctctgcc agcgggatct ccgccacctg tttgcgatcg cgcatgatga tcacccggtc
    44761 ggcatagccc accagttctt ccagttcgga ggagatcacc agcagcgcca gaccatcggc
    44821 gcatagcatt tcaatcaggc ggatgatctc ggcgtgcgca ccaacgtcaa ttccacgcgt
    44881 tggctcgtcg aggatcagaa attgcggacg agtcagcagc cagcgagaga gcagcacttt
    44941 ttgctgattg ccgccggaga gaaattcaat cggttgttca gtggaagggg tgcgaatgcc
    45001 aagctggcgg ataaagcgtt cggcaatctc ttgctgttct ttgcgggaga tcggtcgcag
    45061 ccagccgcgc tgggcctgta gcgcgaggat gatattttcc cgcaccgagg cagcggcgat
    45121 gatgccatcg gttttcctgt cttccgggca gaagccgata cccagtaccg acgcctgatg
    45181 tggcgatcgc agggtttgcg gtttgccttt gatcaacgca gtgccgctgt cagcaggttt
    45241 gataccgaag atcacttcgg cggtttcggt acgtcctgat cccagcaagc cagccagacc
    45301 gacgatctcg ccggggcgta cttcgagatc aaacggtgcg atcgttcctt ttttgccgta
    45361 atttttgaac gcggcaacgg gtttgtcgct caacaatgtt cgcccggcac gctgtagcgc
    45421 gtgggtatcc agctcgcgcc ccagcatcat ttttaccagt tcgatctgcg gtagctcgca
    45481 cgtttcccga cagcctacga aactgccgtt gcgtaagacg gtgatccgat cgctgacctg
    45541 atagacctga tcgagaaagt gagtgacaaa aatcaggctg acgccgcgat cgcgcaactg
    45601 acgcatcagg tcaaacagta actccacttc ctgggtgtcg agactggcgg ttggttcatc
    45661 gaggatcagc actttggcag agagatcgat agcccggcaa atagcgacga tttgctgcat
    45721 cgcgactgaa aagcggttga gcggttcgcg cacgtcgagg gagaaaccgt aagatgccat
    45781 cagttcggtg gcgcgctttt ccatctcttt gcggcgtaga aggccgaagc gtttgggttc
    45841 gcggcctata aatagattat cagcgaccga catattgggt agcaggttta cttcctgata
    45901 gacggtgcca atgccgagtt gttgcgcgtg ggcggtattt ttcggtgaga taggctggcc
    45961 ttccagccag atggtgccgc gatcggcatg gtatacacca gttaatgctt tgattagcgt
    46021 tgattttccc gccccgtttt caccgagcag cgccatgatt tcgccacggc gcaggctgaa
    46081 atcaacgttg tctaacgctt tgacgccggg gaaaaattta cttaatcctt cggtgcggag
    46141 gatctcctgg tgttggtcgg tcgtcatggt ttcccccggt gtaaagcgtg ctcccagtag
    46201 gcatgataag acgcggtaag cgtcgcatca ggcttcagtg caccatcgcc ggatgcggcg
    46261 cgagcgcctt atccggccta cgaatcggca tcaggcatag caaccctcaa taccccatat
    46321 ttttcttctt ctctaactct tcttttgcgg tatcaggcag gtaaagggtg gatttggtta
    46381 acgtcagctt ttcaggcatg gtgccgtctt ttttgtattt ctccagcgcg tcaaaagcgg
    46441 gtcccgccat gttcggcgtc agttcaacac tggcgttcgc ttcgccatcc atcatcgctt
    46501 tgtagatgtc cggtacaccg tcaatggaac ccgtgaggat atctttgccc ggtttcaggc
    46561 ccgcttcttt aattgcctga attgcaccaa tcaccatgtc gtcgttatgg gcgtaaacca
    46621 tgcagatgtt tttgccgttg ttttccgctt tgataaagct ctccatgact tctttgcctt
    46681 tactgcgggt gaagtcacct gactgtgagc ggatgatttt gatatttggc gcattcttaa
    46741 tggcttcggc aaagcctttc ttacggtcaa tggcgacgct ggccccaacg gtgccctgca
    46801 gctccaccac gttgcatggt ttgccattca cttcttttac cagccagtca ccaatcaact
    46861 tgccttcgag gatgttgtcg gcagtaacgg tggtcatata gagagatttg tctttcacat
    46921 cgatggaacg gtcgagcaag aagaccggga tttcggcatc tttcgcctct tttaataccg
    46981 gttcccaacc cgtcgcaacc accggagcga taaagatcgc atccacccct tgtgcaacga
    47041 aggaacgtac cgctttaatc tggttttcct gcttttgctg accatcggca attttcagcg
    47101 taattccgcg tttttcggcc tcacttttcg ccacattggt ttctgcggcg cgccagccgg
    47161 attccgatcc gacctgcgaa aatcctacgg ttaatggagc ggccaacgcc atagacgaca
    47221 tggctgccga gactgcagag actacaagta agcgtttcca catagggttg tcctcgtcgg
    47281 ggtaattatt attggtgaaa agatgttcgc gaaaaaacta tagacaattc gttatgtaac
    47341 ggattgcgtt acatcacact tcaaaaaagt aaataaaacg ttaatcacaa gtttgtaatc
    47401 gctttcatct cactatgaaa aatgcggcta cggttatgga ttttcctgct ctgtataccg
    47461 tcttaaaact ggcgaaaaag gaaaatgaag acgaaaacaa gcgaagacat tcggcgcgag
    47521 ttggctataa tactcggcac ttgtttgcca catattttta aaggaaacag acatgagctt
    47581 actcaacgtc cctgcgggta aagatctgcc ggaagacatc tacgttgtta ttgagatccc
    47641 ggctaacgca gatccgatca aatacgaaat cgacaaagag agcggcgcac tgttcgttga
    47701 ccgcttcatg tccaccgcga tgttctatcc atgcaactac ggttacatca accacaccct
    47761 gtctctggac ggtgacccgg ttgacgtact ggtcccgact ccgtacccgc tgcagccggg
    47821 ttctgtgatc cgttgccgtc cggttggcgt tctgaaaatg accgacgaag ccggtgaaga
    47881 tgcgaaactg gttgcggttc cgcacagcaa gctgagcaaa gaatacgatc acattaaaga
    47941 cgttaacgat ctgccagagc tgctgaaagc gcagatcgct cacttcttcg agcactacaa
    48001 agacctcgaa aaaggcaagt gggtgaaagt tgaaggttgg gaaaacgcag aagccgctaa
    48061 agctgaaatc gttgcctcct tcgagcgcgc caagaataaa taagttcttc tagcgcaata
    48121 accctgaacg ccgggcttcg gttagtaagg gtttttttat gcccgcgata aataaacaca
    48181 ccttattcca ccaccgcctg caagcgtaat aacgcctctt ccaccacctc atccgcagcc
    48241 agaccaatac gctttgccag ccgggcgcgt agatccatca tccgcacctg attaaccagc
    48301 accacgccgt gcacatcgcc ttcttcgcaa tgtaaaggaa cgctaaatcc ggcataacgg
    48361 gcaaaatttc caccctgcgt aatgggggcc accagcgtca ttcccagttg attaaaggct
    48421 tgaacggaga gcacaagcgc aggtcgacca gcaccttgct gttcatggct gcttgctgga
    48481 tcaaagccaa ccagcacaat gtctccccgt tcaaattcac ttttctttac catatttcgt
    48541 cacccgcagg ggtggattta ccccagacat cctgctcgct aagttccgcg gcgttcatgt
    48601 cacactgtgc cagcagttca tcaagcgagt agcgcctgga gatgggtgtc agaatcagtt
    48661 ggttgttgct cacctgcgct tccacgctct gccccggctg taagttaagt tctttcatta
    48721 cgatattggg aatgaccata cctgcactgt tcccccatct ttttatggta atacgcatct
    48781 gcacctcctt aagttataca aagtataact cacggtgcta caggaaacgt tttacccctg
    48841 cgcatttgtg aatgccagca gagtttttta tgcgttaata gatatggaaa gcggctcgga
    48901 gagaaaagca aaaggtgagg gaattacaaa acagaatgcg agtcgtctcg caaaaaacaa
    48961 cgccacccga aggtggcata agggggtagc atgtaccgca ctaccccttg atatatcaaa
    49021 tagttacaag ttcaaagtgg ttcgaaatcg tgccatttag tccccgttta tgggcaaaat
    49081 ctggacaact ccgcaaccgg attgaacctt accgcgtcct ggagataatc aggtgcaaag
    49141 tgcgcatagg tcattgtttg ctgaattgtg ctgtgcccta aaatacgctg aagggtaaca
    49201 atgttccctc catttatcat gaaatgagtt gcgaatgtgt gccgtaaaac atgcacagac
    49261 tgaccgactg gtaaatcagg ccgcacagtc cttattgcac ttctgactat cgcataactg
    49321 gggttcatta atcttccagt cgctttgact ttaatcagag attcaagatc tttagctata
    49381 ggaataaccc gccgcgatcc attctttgtc tccatgaaaa taaccttccc accaattatg
    49441 tgttcacctt taatattggc aacttcaccc cagcgcccgc ctgtagccag gcttaacaat
    49501 acagccttaa gattatcgcc agataaaagt gcggttagcc tgggaatatc atcatgtgca
    49561 agataagcca tttccggctg attaattgtt agcttgcgta tctcgtgaaa cgggttatca
    49621 cccacaaact cttcaacatc aatcagcttt tcaaagaaac cgctcatcat tgcacaataa
    49681 cgatttaccg ttgactgctt aattccgcga ttcattaatt caaggcgata tctgataatg
    49741 ttttttcggg ttaagtggtc acttctgtta ataccaagct cttgcatatc acgaagaacc
    49801 gcttcaattc tgacccgatt catcgcgcca taacgatgat tcttaccgtg gtacacccac
    49861 cacagctcca gaagatcgct taacgcttta cgttcaatag gtttttcgac ccagtcctta
    49921 tcgtgataat gcgtcagaac atggcgttca tacatcgccg cttcaccacg ggtaggaaat
    49981 cgccgccgaa atcttcttcc ttcagatcca gcaggccgca cgtccacaca gtatttgcca
    50041 tcagcgagct tcttaatcga cataaggaag ccccccaatg aaaacatcat cttgatcaca
    50101 attttgagaa ataaaggcca gatggaccgt taaccagtct tcttgccgga gtgggatgat
    50161 tccgcttcgc tttgcccaat gtgtgcgaga gccggtacca cctgcccggc ctttggttcg
    50221 acctcatcga acaaaaacca gtcacggtat ttagaaaatc taggatgctt gaaaagcttg
    50281 atggtgatct caaggccagg aaggttacga ccatactcat aactggtcag agcagaataa
    50341 gaaattccaa tcatatcagc gaattcccgc tgagtgagat cttcagattt acgcatgacg
    50401 cgaatttttt gaggaatatc catttgacaa actcctacat atgtgtagat taacaccaca
    50461 tatgaacagc aaacaaaatg ttctaagtgg ttccaaatag tgccaattag aaaaagcacc
    50521 aatggcggag gatatcagat gcaagaaagc actcaaaacg cgccggagtc actttgtggc
    50581 acacgatgcg atcaccagtc agagatatcg gaagcagaac acctgaagac cggccgcaaa
    50641 gcccctgaac gccaggaaat ccggctttca caaaaccctt ctcatctcct gtcaaaagaa
    50701 gggtttgcga tgtatatcgg caagaccgtt gacgccatcg tgagcatggc aaaagcaggg
    50761 aaattacccg ctgtgtacat ggcggaccct ctcaagcctg gcggaaacgc cgaactctac
    50821 atcaaccgga aagcgtggga tgaagcctgc gatcgactca ctgaaaacgc accgcaagaa
    50881 tggcacgaat gggaaaaccg acttttttta tttaagccag cagctggttt acggaataaa
    50941 cgtatcagta aaaaagcagc ttaatatcaa acacacagcg aaatccacaa caggagattt
    51001 cgccgtaaat aatctaatcg ttggtgttat gcgggggcgc aacaatcaac caagaagact
    51061 tttgaaagca aaggataacc cagtagtaag agcggatgat gcggtgcttt ttatcgcatc
    51121 cattgaacca ctcttggctg catcaacaag cttgtcgcca aatgattgtt taagtgactc
    51181 tggatcaagc ttaagtaatt caagaccttt ggccgtgaga accgcatcag aaaagtaatg
    51241 cggaggagaa gatttagcgg atacataacc agcatcaaca agccatgcga tggagtcacg
    51301 gcgaagctca agagcttcaa ctagttgcct cctggaggat cggtcttctt caaaagtcag
    51361 ctctgaaaca ccggttaagg cacaaatatc aatagttact ttgcggggaa aagtttcata
    51421 aagaaacgca aaagttttac caactgtttc attaaaaaca tcaagatttg aaggtattga
    51481 catgactgac tccactcaaa caaaagactt atacagcgat ttacgcttca tcatcataca
    51541 acagcaatca ggcaggatgg atccaacacg tgcaagcaag gcctggaaag aagcccttac
    51601 cgatcagggg atcgatgtaa ttagcgaagt catggctgaa gtgattttta ggctgacaag
    51661 agacgaatca ctgtaatcat cgcataagga ccgagtgaag aatgaagcaa caacgaaatg
    51721 cacgcatcaa taacaacacc gaacgccacg ttgcacgcca gcacttccgt aaaaatagtg
    51781 cgaccgtaac cactgaaatc catgccacac cagacggtca cccggtaaaa caaaccggaa
    51841 agcacacctg ggcgattggc gacactggca tcgttattca caaaagtttt cgcagccctg
    51901 ttactggcag atttaatttc accctgactc gcggggatga ttacttcgga caggacttca
    51961 cattttttga agccttaaga acggcagaca gattaatcag cggcctgcgt tttcaatatc
    52021 cgggaagcaa gcactgataa ataaaggatt cagccatgat cgaagcctac aaaatactta
    52081 tcaataacgc gttgcaacgt tatcactttg aactcgcaaa ccggggcgta actaaggatg
    52141 aaagccttcg taactcactg cgcggcgcaa ttatggggct atacaactgc gcactgggca
    52201 gcgatgaccg tgaagcgctg gaagaattaa agtcgattat gacagaactg tttgaaggtg
    52261 aaattgtcga gccttacttc acaggggtag c
//
LOCUS       DAZGCQ010000024        51231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000024 DAZGCQ010000000
VERSION     DAZGCQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.024"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..1513)
                     /gene="rhlE"
                     /locus_tag="ACHK07_001503"
     CDS             complement(149..1513)
                     /gene="rhlE"
                     /locus_tag="ACHK07_001503"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO8101855.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1742..2413
                     /gene="cecR"
                     /locus_tag="ACHK07_001504"
     CDS             1742..2413
                     /gene="cecR"
                     /locus_tag="ACHK07_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO8101856.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2416..3411
                     /gene="hlyD"
                     /locus_tag="ACHK07_001505"
     CDS             2416..3411
                     /gene="hlyD"
                     /locus_tag="ACHK07_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO8101857.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3404..5140
                     /gene="ybhF"
                     /locus_tag="ACHK07_001506"
     CDS             3404..5140
                     /gene="ybhF"
                     /locus_tag="ACHK07_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8101858.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5133..6266
                     /gene="ybhS"
                     /locus_tag="ACHK07_001507"
     CDS             5133..6266
                     /gene="ybhS"
                     /locus_tag="ACHK07_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8101859.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6277..7383
                     /gene="ybhR"
                     /locus_tag="ACHK07_001508"
     CDS             6277..7383
                     /gene="ybhR"
                     /locus_tag="ACHK07_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8101860.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTYQTVPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7345..7755)
                     /gene="ybhQ"
                     /locus_tag="ACHK07_001509"
     CDS             complement(7345..7755)
                     /gene="ybhQ"
                     /locus_tag="ACHK07_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO8101861.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7888..8649
                     /gene="ybhP"
                     /locus_tag="ACHK07_001510"
     CDS             7888..8649
                     /gene="ybhP"
                     /locus_tag="ACHK07_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8101862.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8646..9887
                     /gene="clsB"
                     /locus_tag="ACHK07_001511"
     CDS             8646..9887
                     /gene="clsB"
                     /locus_tag="ACHK07_001511"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO8101863.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9887..10843
                     /gene="ybhN"
                     /locus_tag="ACHK07_001512"
     CDS             9887..10843
                     /gene="ybhN"
                     /locus_tag="ACHK07_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO8101864.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGENTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10879..11592)
                     /gene="ybhM"
                     /locus_tag="ACHK07_001513"
     CDS             complement(10879..11592)
                     /gene="ybhM"
                     /locus_tag="ACHK07_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8101865.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11798..12502)
                     /gene="ybhL"
                     /locus_tag="ACHK07_001514"
     CDS             complement(11798..12502)
                     /gene="ybhL"
                     /locus_tag="ACHK07_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8101866.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12638..13090)
                     /gene="moaE"
                     /locus_tag="ACHK07_001515"
     CDS             complement(12638..13090)
                     /gene="moaE"
                     /locus_tag="ACHK07_001515"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO8101867.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13092..13337)
                     /gene="moaD"
                     /locus_tag="ACHK07_001516"
     CDS             complement(13092..13337)
                     /gene="moaD"
                     /locus_tag="ACHK07_001516"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO8101868.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13330..13815)
                     /gene="moaC"
                     /locus_tag="ACHK07_001517"
     CDS             complement(13330..13815)
                     /gene="moaC"
                     /locus_tag="ACHK07_001517"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO8101869.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13818..14330)
                     /gene="moaB"
                     /locus_tag="ACHK07_001518"
     CDS             complement(13818..14330)
                     /gene="moaB"
                     /locus_tag="ACHK07_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO8101870.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14352..15341)
                     /gene="moaA"
                     /locus_tag="ACHK07_001519"
     CDS             complement(14352..15341)
                     /gene="moaA"
                     /locus_tag="ACHK07_001519"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO8101871.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15329..15473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15738..16646
                     /gene="yvcK"
                     /locus_tag="ACHK07_001520"
     CDS             15738..16646
                     /gene="yvcK"
                     /locus_tag="ACHK07_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO8101872.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16837..18858)
                     /gene="uvrB"
                     /locus_tag="ACHK07_001521"
     CDS             complement(16837..18858)
                     /gene="uvrB"
                     /locus_tag="ACHK07_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO8101873.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19437..20114)
                     /gene="bioD"
                     /locus_tag="ACHK07_001522"
     CDS             complement(19437..20114)
                     /gene="bioD"
                     /locus_tag="ACHK07_001522"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8101874.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPRLAENPENAATGK
                     YINLALL"
     gene            complement(20107..20862)
                     /gene="bioC"
                     /locus_tag="ACHK07_001523"
     CDS             complement(20107..20862)
                     /gene="bioC"
                     /locus_tag="ACHK07_001523"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO8101875.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLVWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYRHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20849..22003)
                     /gene="bioF"
                     /locus_tag="ACHK07_001524"
     CDS             complement(20849..22003)
                     /gene="bioF"
                     /locus_tag="ACHK07_001524"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO8101876.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCSGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            complement(22000..23040)
                     /gene="bioB"
                     /locus_tag="ACHK07_001525"
     CDS             complement(22000..23040)
                     /gene="bioB"
                     /locus_tag="ACHK07_001525"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO8101877.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23127..24416
                     /gene="bioA"
                     /locus_tag="ACHK07_001526"
     CDS             23127..24416
                     /gene="bioA"
                     /locus_tag="ACHK07_001526"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO8101878.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIIHPQQLQRLTAGVNRAVQDETFFCQ"
     gene            24475..24951
                     /gene="ybhB"
                     /locus_tag="ACHK07_001527"
     CDS             24475..24951
                     /gene="ybhB"
                     /locus_tag="ACHK07_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO8101879.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25103..26386
                     /gene="ybhC"
                     /locus_tag="ACHK07_001528"
     CDS             25103..26386
                     /gene="ybhC"
                     /locus_tag="ACHK07_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO8101880.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26527..28788)
                     /gene="ybhJ"
                     /locus_tag="ACHK07_001529"
     CDS             complement(26527..28788)
                     /gene="ybhJ"
                     /locus_tag="ACHK07_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO8101881.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSSIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28971..30404)
                     /gene="ybhI"
                     /locus_tag="ACHK07_001530"
     CDS             complement(28971..30404)
                     /gene="ybhI"
                     /locus_tag="ACHK07_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO8101882.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30480..31532)
                     /gene="ybhH"
                     /locus_tag="ACHK07_001531"
     CDS             complement(30480..31532)
                     /gene="ybhH"
                     /locus_tag="ACHK07_001531"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO8101883.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31716..32669
                     /gene="ybhD"
                     /locus_tag="ACHK07_001532"
     CDS             31716..32669
                     /gene="ybhD"
                     /locus_tag="ACHK07_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8101884.1"
                     /translation="MKHELSSMKAFVILAESNSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32710..33705)
                     /gene="pgl"
                     /locus_tag="ACHK07_001533"
     CDS             complement(32710..33705)
                     /gene="pgl"
                     /locus_tag="ACHK07_001533"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO8101885.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33860..34678
                     /gene="ybhA"
                     /locus_tag="ACHK07_001534"
     CDS             33860..34678
                     /gene="ybhA"
                     /locus_tag="ACHK07_001534"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO8101886.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34679..35737)
                     /gene="modC"
                     /locus_tag="ACHK07_001535"
     CDS             complement(34679..35737)
                     /gene="modC"
                     /locus_tag="ACHK07_001535"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO8101887.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35740..36429)
                     /gene="modB"
                     /locus_tag="ACHK07_001536"
     CDS             complement(35740..36429)
                     /gene="modB"
                     /locus_tag="ACHK07_001536"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO8101888.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36429..37202)
                     /gene="modA"
                     /locus_tag="ACHK07_001537"
     CDS             complement(36429..37202)
                     /gene="modA"
                     /locus_tag="ACHK07_001537"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8101889.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37369..37518)
                     /gene="acrZ"
                     /locus_tag="ACHK07_001538"
     CDS             complement(37369..37518)
                     /gene="acrZ"
                     /locus_tag="ACHK07_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO8101890.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37647..38435
                     /gene="modE"
                     /locus_tag="ACHK07_001539"
     CDS             37647..38435
                     /gene="modE"
                     /locus_tag="ACHK07_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO8101891.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38503..39975
                     /gene="modF"
                     /locus_tag="ACHK07_001540"
     CDS             38503..39975
                     /gene="modF"
                     /locus_tag="ACHK07_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO8101892.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            40237..41253
                     /gene="galE"
                     /locus_tag="ACHK07_001541"
     CDS             40237..41253
                     /gene="galE"
                     /locus_tag="ACHK07_001541"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO8101893.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPMFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMCAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41263..42309
                     /gene="galT"
                     /locus_tag="ACHK07_001542"
     CDS             41263..42309
                     /gene="galT"
                     /locus_tag="ACHK07_001542"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO8101894.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42313..43461
                     /gene="galK"
                     /locus_tag="ACHK07_001543"
     CDS             42313..43461
                     /gene="galK"
                     /locus_tag="ACHK07_001543"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO8101895.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43455..44495
                     /gene="galM"
                     /locus_tag="ACHK07_001544"
     CDS             43455..44495
                     /gene="galM"
                     /locus_tag="ACHK07_001544"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO8101896.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHLWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            44697..45449
                     /gene="gpmA"
                     /locus_tag="ACHK07_001545"
     CDS             44697..45449
                     /gene="gpmA"
                     /locus_tag="ACHK07_001545"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO8101897.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45607..46659)
                     /gene="aroG"
                     /locus_tag="ACHK07_001546"
     CDS             complement(45607..46659)
                     /gene="aroG"
                     /locus_tag="ACHK07_001546"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO8101898.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46975..47355
                     /gene="ybgS"
                     /locus_tag="ACHK07_001547"
     CDS             46975..47355
                     /gene="ybgS"
                     /locus_tag="ACHK07_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO8101899.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47469..48410
                     /gene="zitB"
                     /locus_tag="ACHK07_001548"
     CDS             47469..48410
                     /gene="zitB"
                     /locus_tag="ACHK07_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO8101900.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48407..49126)
                     /gene="pnuC"
                     /locus_tag="ACHK07_001549"
     CDS             complement(48407..49126)
                     /gene="pnuC"
                     /locus_tag="ACHK07_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO8101901.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49164..50207)
                     /gene="nadA"
                     /locus_tag="ACHK07_001550"
     CDS             complement(49164..50207)
                     /gene="nadA"
                     /locus_tag="ACHK07_001550"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO8101902.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50485..50610)
                     /locus_tag="ACHK07_001551"
     ncRNA           complement(50485..50610)
                     /ncRNA_class="other"
                     /locus_tag="ACHK07_001551"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(50641..50716)
                     /locus_tag="ACHK07_001552"
     tRNA            complement(50641..50716)
                     /locus_tag="ACHK07_001552"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50681..50683),aa:Lys,seq:ttt)
     gene            complement(50750..50825)
                     /locus_tag="ACHK07_001553"
     tRNA            complement(50750..50825)
                     /locus_tag="ACHK07_001553"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50790..50792),aa:Lys,seq:ttt)
     gene            complement(50972..51047)
                     /locus_tag="ACHK07_001554"
     tRNA            complement(50972..51047)
                     /locus_tag="ACHK07_001554"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51012..51014),aa:Lys,seq:ttt)
     gene            complement(51051..51126)
                     /locus_tag="ACHK07_001555"
     tRNA            complement(51051..51126)
                     /locus_tag="ACHK07_001555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51091..51093),aa:Val,seq:tac)
BASE COUNT        12416 a        13578 c        13050 g        12187 t
ORIGIN      
        1 gaaataattt cattgccatt ttagatacac tagatagatc ataaagcaaa tttattaaaa
       61 caataaaaac agacatgtca tgcatgtctg accgtgttac ctgaataaag ggcaaaagcc
      121 tgtgatactc ttttgccctt acatggtatt actgcgcagc ggcaggttta cgcggacggc
      181 ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac ggtttttctg
      241 caggcttcgc gcttgcagat tttgcaccac cttctccacg gcgtagttgc tgttgaccgc
      301 gaccaccgcc ctgcccacga ccaccgccgc cacgttgctg gcgaccgttc tggatcggtt
      361 cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg atctcttttt
      421 tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc aacgacaacg
      481 cttctccggt ggctgcggca cgaccggtac gcccgatacg gtggacataa tcttccggta
      541 cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga ccgcgcgcag
      601 cgatgtcagt tgccaccagt acacgaatat cgcccgattt aaaatcagcc agcgcacggg
      661 tacgcgcacc ttgcgattta ttaccgtgga tcgccgcact acggatgcca tctttattaa
      721 gctgttcagc gagatggtta gcgccatgtt tggtacgggt aaacaccagc acctgctgcc
      781 agttcccttt cccaatcatg tgcgacagca attcgcgttt gcgcttctta tcaacaaagt
      841 gaacatgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg atttccagcg
      901 ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc gcggagaaca
      961 acaggttctg gcgcttcgca ggtagttttg ttaacacgcg acggatatcg tggataaagc
     1021 ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga tccagcttca
     1081 ctgcattctg atgttccagg tccagcaaac gtcccggcgt tgccaccagc acatcaacgc
     1141 cgccgcgcaa cttcatcatt tgtgggttaa tgctgacacc gccaaacacc accagcgaac
     1201 gaatgttcag atatttgctg taatcacgga cgttttcgcc aatctgcgca gccagttcac
     1261 gggtcggggt aagaatgaga gcacgtaccg gacgacgccc tttggcgtgc ggctggcgag
     1321 tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttgccggtg ccggtctggg
     1381 cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc tgaatagggg
     1441 tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt aaacccaaag
     1501 aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt tttcagggag
     1561 atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt gtagcagttt
     1621 ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct tagccatact
     1681 ggacaactcc cctatggagt cataatctta atcaatcatt tgattaagaa tggactgtgc
     1741 gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc tgattgctgc
     1801 cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga tagccgccca
     1861 ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag atttgtacct
     1921 cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc atgccgagga
     1981 agccgaacgc ttgttcgccc aaccacagcc tgatcgggct gccatccgtg aactgatcct
     2041 tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca acctcagcaa
     2101 gtttatctcc cgtgagcagc tctctcccac ggcagcctac cacctggtgc acgaacaggt
     2161 gattagtccg ctacacagcc acctgacacg cctgattgcc gcctggaccg gctgcgacgc
     2221 caatgacacc cgcatgatcc ttcataccca tgcgttgatt ggtgagattc tggcgttccg
     2281 tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg aagaaaagac
     2341 cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag gattatcgca
     2401 aaggagtttg tagtgatgaa aaaacctgtc gtgatcggac tggcggtagt ggcacttgcc
     2461 gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg cctgacgctg
     2521 tatggcaacg tggatattcg tacggtgaat cttagtttcc gtgttggggg gcgcgttgaa
     2581 tcgctggcgg tggacgaagg tgatgcgatc aaagcgggcc aggtgctggg cgaactggat
     2641 cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gcgtctcggt ggcacaggcg
     2701 cagtatgacc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc cgccgctgcg
     2761 gtgaaacagg cgcaagccgc ctatgactat gcgcagaact tctataaccg ccagcaagga
     2821 ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc ctcgcgcgac
     2881 caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg ttccggtaac
     2941 cgtgaacagg acatcgccca ggcgaaagcc agcctcgaac aggcgcaggc gcaactggcg
     3001 caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg cacgctgtta
     3061 acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt taccgtttca
     3121 ctaacgcgtc cggtgtgggt gcgcgcgtat gttgatgaac ggaatcttga ccaggcccag
     3181 ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta tcacgggcag
     3241 attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac accggatctg
     3301 cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga tgcgttacgc
     3361 cagggaatgc cagtgacggt acaattcggt gacgaggcag gacatgaatg atgccgttat
     3421 cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg tcgcgccgct
     3481 cgattgcacc attcatgccg gttatgtgac ggggctggta gggccagacg gtgcgggtaa
     3541 aaccacgctg atgcggatgc tggcgggatt actgaaacct gacagcggca gtgccacggt
     3601 gattggcttt gatccgatca aaaatgacgg cgcgctgcac gccgtgctcg gctatatgcc
     3661 gcagaaattt ggtctgtatg aagatctgac ggtgatggag aacctcaatc tgtacgcgga
     3721 tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc tggagtttac
     3781 gtctcttggc ccgtttaccg gacgcctggc gggcaagctc tccggtggga tgaaacaaaa
     3841 actcggcctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg atgaacccgg
     3901 cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg agctggcggg
     3961 cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc agtgccgtga
     4021 cgtgttactg atgaacgaag gcgagttgct gtatcaggga gaaccaaaag ccctgacaca
     4081 aaccatggcc ggacgcagct ttctgatgac cagtccacac gagggcaacc gcaaactgtt
     4141 gcaacgcgcc ttgaaactgc cgcaggtcag cgacggcatg attcagggga aatcggtacg
     4201 tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg ggatgccgga
     4261 aatcaacatc aacgaaacta cgccgcgttt tgaagatgcg tttattgatt tgctgggcgg
     4321 tgccggaacc tcagaatcgc cgctgggtgc aatattgcat acggtagaag gcacacccgg
     4381 cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg ccgccaccga
     4441 tcacgtcaac tttgccgtta aacgcgggga gatttttggt ttgctggggc caaacggcgc
     4501 gggcaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt ccggccaggc
     4561 gctggtgctg gggatggatc tgaaagagag ttccggtaaa gcgcgccagc atctcggcta
     4621 tatggcgcaa aaattttcgc tctacggcaa cctgacggtc gaacagaatt tacgcttttt
     4681 ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc gtatgagcga
     4741 ggcatttggc ctgaaaagta ttgcctcaca cgccaccgat gaactgccat taggttttaa
     4801 acagcggctg gcgctggcct gttcgctgat gcatgaaccg gatattctgt ttctcgacga
     4861 accgacttcc ggcgttgacc ccctcacccg ccgtgaattc tggctgcaca tcaacagcat
     4921 ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag cggaatattg
     4981 cgaccgcatc ggcctggtgt accgcgggaa attaatcgcc agcggcacgc cggacgattt
     5041 gaaagcacag tcggctaacg atgagcaacc cgatcccacc atggagcaag cctttattca
     5101 gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt tcctggcgtc
     5161 gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg agtagctggc
     5221 tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc attaacctcg
     5281 actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg gcgctggatt
     5341 tcacccacac tatgaccggt tcgccctaca tcgacgccac catcagcgat aaccgtcagg
     5401 aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt ccggtggatt
     5461 ttgcggaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc accgacggca
     5521 gtgagccgaa taccgctaac tttgtacagg ggtatgtcga agggatctgg cagatctggc
     5581 aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat gtacaaaccc
     5641 gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt gcggtgacca
     5701 tcatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcacga gaatgggaac
     5761 gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg ctgctgtgta
     5821 agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg ctggtgtcag
     5881 tgtttattct cggcgtgccg tatcgcggtt cgctgctgat tctgtttttt atctccagcc
     5941 tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc cgcaaccagt
     6001 tcaatgccgc tcaggtcgcc ctgaacgccg cgttcctgcc gtcgattatg ctttccggct
     6061 ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac attattcccg
     6121 ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt ccagtggtgc
     6181 tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc ctgacgtggc
     6241 tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt atggacgtta
     6301 atccgcaaag agttgcagtc gttgctgcgc gaaccacaaa cccgcgcgat tctgatttta
     6361 cccgtgctaa ttcaggtgat cctgttcccg ttcgccgcca cgctggaagt gactaacgcc
     6421 accatcgcca tctacgatga agataacggc gagcattcgg tggaacttac tcaacgtttt
     6481 gcccgcgcca gcgcctttac acatgtgctg ctgctgaaaa gcccacagga gatccgcccc
     6541 accatcgaca cacaaaaggc gttactactg gtgcgtttcc cggctgactt ctcgcgcaaa
     6601 ctggatacct accagaccgt gccgttgcag ttgatcctcg acgggcgtaa ctccaacagt
     6661 gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca ggaactgctg
     6721 gaaggaaaac cgaaacccaa caacagcgag ctggtggtac gcaactggta taacccgaat
     6781 ctcgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac tatcggcgta
     6841 atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct cgatcagcta
     6901 ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc ggcgttaatt
     6961 gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta tcaaatcccc
     7021 ttcgccggat cgctggcgct gttctacttt acgatggtga tttatggttt atcgctggtg
     7081 ggattcggtc tgttgatttc atcactctgc tcgacacaac agcaggcgtt tatcggcgtg
     7141 tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt ggaaaacatg
     7201 ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac ggacattacc
     7261 aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt gtggccgcta
     7321 ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg taaggtgatg
     7381 taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga ttgccagccc
     7441 tgcaattgcc agcaaaaagt tgttttccag aacgcgggac aacagccaca gcacaatcag
     7501 cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca cctcacgcca
     7561 gcgttgctcg gggtggcgct gaaacaccag catcatcact accgtgacac aatacagtgc
     7621 gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca ccagcagcgc
     7681 cactaccagt aaatgcaaca taatctgcca gcaacttaga cccgttgcga cacgaacacg
     7741 ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc agagtaccga
     7801 actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca ctatttcaat
     7861 gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt ttcgttcaag
     7921 gtgctcacca tcaatattca caaaggcttt accgcgttta accgacgctt cattttgccg
     7981 gaacttcgcg atgccgtgcg taccgtcagc gccgatattg tttgcctgca ggaagtgatg
     8041 ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc gcactacgag
     8101 tttctcgccg acactatgtg gagcgatttt gcctacggtc gcaatgccgt atacccggaa
     8161 gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga gaatcgcgat
     8221 gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt gccgccgatg
     8281 accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc gcaccgtcag
     8341 gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga accggtattg
     8401 gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa agtgcaggcc
     8461 ggactggatg agatttttac ccgcgcccac ggacgcccgg cgcgcacgtt tccggtgcaa
     8521 tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag cgcgccaacc
     8581 gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag tgcggagatt
     8641 catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa aacggcgagc
     8701 aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc attcttgaaa
     8761 cgtttatctg gtttgaggat gacgtcggca aacaactgca tgcggcacta ctggcagcag
     8821 cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg gatctcagcg
     8881 atgagtttgt caatgaactg acggcagctg gcgtggtgtt ccgctactac gatccccgcc
     8941 ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa attgtggtga
     9001 tcgatgcgcg tatcgccttt attggcgggc tgaattattc cgccgagcat atgtccagct
     9061 acggtccaga ggccaaacag gattacgcgg tgcgccttga agggccgatt gtcgaagata
     9121 ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc tggtggcgac
     9181 gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg ctggtctggc
     9241 gcgataacga agaacatcgc gatgatattg aacgtcacta tctgaaaatg ctcactcagg
     9301 cgcgtcggga agtgattatt gccaacgcct acttcttccc cggctatcga tttttacacg
     9361 ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag ggcgaaccgg
     9421 atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt aaaggcggcg
     9481 ttcaggtgtt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg atggacgatc
     9541 actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg aatctcgaag
     9601 caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat ctgaacggca
     9661 ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc acctggtgga
     9721 acctaaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg ctggttggct
     9781 ggcttccggc acacacgcca cgtctggcgc aggttgatcc ccccgcacaa ccgacaatgg
     9841 aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga gtaaatcaca
     9901 cccgcgctgg cgcttagcaa agaagatcct cacctggctg ttttttatcg cggtgattgt
     9961 gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg tcatccgcga
    10021 ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct atctgattta
    10081 cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga agcgccaggt
    10141 gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct gggtcggcgg
    10201 cattggtatg cgctatcgtt tgtactctcg tctggggttg cccggtagca ctattacgcg
    10261 gattttctcg ctcagtatta ccaccaactg gctgggctat attttgctgg cggggattat
    10321 ctttaccgca ggcgtggtgg agctaccgga tcactggtat gtcgatcaaa ccacactgcg
    10381 cattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt gcgctttcgc
    10441 gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccctcat ggaaattcgc
    10501 cctcgcccag atgttgattt ccagtgttaa ctggatggtc atgggagcga ttatctggct
    10561 gttacttggt caaagcgtga actatttctt tgtactgggc gtgttgctgg ttagtagtat
    10621 tgcaggcgtc atcgtgcata ttccggcggg gattggtgtg ctggaagcgg tatttatcgc
    10681 attactggca ggggagaata catccaaagg cacaattatc gccgccctgc tcgcttatcg
    10741 cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc tactggaaag
    10801 ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaagttcag ttaagttctt
    10861 tagtatgtgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga caagcgttat
    10921 agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg ttaatgcacc
    10981 tataaaagcg caacgaaccg gactccacaa ctcttcacta tggcatttcc ccgccagttc
    11041 aaggagctta tttcgtccat gcgagataat gcctgaccat aacacaatca ttaagcaact
    11101 tattacccaa atgggccgtt cactcattaa tgccgcattc acgatgatta ccagggccaa
    11161 ccctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat taaataataa
    11221 accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga tgataatggc
    11281 atatacagga actaaacttg caacgacaat cgccaccaaa gagagtaaaa acagatatat
    11341 tacgcctgcg atggcggcat ttatcttgtt gatatcctta cttaagccat acatacaaag
    11401 taataataac cctatggcga catacattgg tggtgaatca tatttggttg ccgtaatttc
    11461 gagatactta gcaccccagg ccagccctgc ggttgccacc aaacctaaag cggtaatgag
    11521 ccatataccg tttaatatcc tggtctcgtg ctgaaaatct aatttattgc tgttttgcaa
    11581 gtatgattcc attcttttag ttgtccttat agaaaaatat taatattcat catgaataaa
    11641 tttaaaccca cgcatacaaa atacttttgt atttatggat acggaaaacg cttccgctcg
    11701 gccataaggc atattttata cctggtactt gttaagtgtc gtttttagta agaaaaaaaa
    11761 tcccggcacc aggccgggat cagcaggaag aagagaatta acgtcgattg ccaaaaattc
    11821 gcagcaacat caggaacagg ttgatgaagt ccagatacaa ggttaacgcg ccaagaatgg
    11881 aatatttgcg caggttcgac gcgtcgcggg tatctatctg ctcacccata tttttcagtt
    11941 tctgggtgtc atacgccgtc aatccgacaa agacaatcac gccgatgtag gtaactgccc
    12001 acatcaatgc ttcgcttttc agccagaagt tgaccagcga tgccagcaca atgccgatta
    12061 acgccataaa cagcatattg ccaaagccac ttaaatcgcg cttcgtggtg taaccgtaca
    12121 ggctcattgc gccgaacatc ccggcagtaa cgacgaaggt actggcgata gaagcagcgg
    12181 tataaacaat gaaaatactg gaaagcgtaa gacccgtcag cgccgaataa agcataaaga
    12241 gcatcgtcgt tacacctgcg ctcagctttt gaatcatcgc tgataacaca ataaccaatg
    12301 ctaattgcgc gatgatcaga ccgattaaaa agacacggtt agtgaacaac agctccatca
    12361 cggccgcgga attagccgca taccaggcaa caaatgcggt cagcaacaag ccaacggtca
    12421 tccagccata gacttgcgcc atataagtct gcaagccagc ccggggttgt acaattgaat
    12481 cagaacgtgg gaatctgtcc atgacgatct cctgaagata taaggaatat cttaaggata
    12541 ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg catgtactga gcggtgaaat
    12601 tgccggacgc agcggtgcct tatccggcta acaaaaacta ccagcgtttt gccgcctgct
    12661 gatcgctctc ccgagcttcg acccagcggt cgccctccgg cgtggcttcg cgcttccaga
    12721 acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac gcactgctgc
    12781 gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc cggccataat tccccgatgc
    12841 ggtgaatcac cgtgacgcgc cccagcggcc agcggttacg cgcttcatcg acaatttctg
    12901 ccagtgcttt ttcagtcatc cccggatagt gttcgagggt taatgctttg acgctgtcgc
    12961 ccaggttatg gttgcgcacc ttaccggtaa aggtgactac cgcaccgtct tcgtcacgct
    13021 ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca acaattttgg
    13081 tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc gccgtcagtc
    13141 agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt gccatcttcc
    13201 agcgccagcg cccagcgatc gctctgcgca gccaggtgct ggcgtaacgc ttcaacggtt
    13261 gggaaatccg cagccacttc ggttgcatct gttcctacca actcgcgcac ctgggcgaaa
    13321 aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc cgctcttcgc
    13381 cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca tgtcataaat
    13441 ggtcagcgcc gccacggagg ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt
    13501 caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct gtaaattgac
    13561 ttcaactttg ctgagcatca gcggatgaca cagcgggatc agatcccagg tgcgttttgc
    13621 cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc
    13681 aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg
    13741 cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg cgttgatatg
    13801 ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg acgcgtacgg
    13861 gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt ggtcgaaccc
    13921 ggcatggcga aaatcagcgt tttgttagcg acgcccgcta ccgcacgaga ttgcaacgtg
    13981 gaagtgccaa tctcttcaaa cgacaacata cggaacactt caccaaaacc ttcaacttca
    14041 cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag gccagtcccc
    14101 cccgtaatca acaccacttg tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga
    14161 atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc
    14221 gccgaatcgc gcaaatagtg accggaggta tcgtcttctt caccgcgacg attagaaacc
    14281 gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat ctgatctctc
    14341 cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt gttgttttga
    14401 tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc
    14461 tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc accaaacagg
    14521 cagagatgga gtttaccaat ggaggaaacg cgcagacggt tgcaagtggc gcagaagtct
    14581 ttttcatacg gcatgataag gccaatctct ccggcgtaat ccggatgaca aaagacttgc
    14641 gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag tagctcgtca
    14701 cgcagaacct gaccagagat gtgatgctta cggaagagct cgctgccctc gcccgtttcc
    14761 atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag aaaggtgtcg
    14821 agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt ctcaaaaccg
    14881 gcctcaaatg cagcatcaat ccctgccatc acctggttga atttatcctg cccggtaata
    14941 gcgtgaaact ggcgggcgtc cagactatca acgctgacgt tgatgccagt aagtccagca
    15001 tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac cgctatctgg
    15061 cgaatagcgt cgttttctcg cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac
    15121 ggctcaccgc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc gcgcgtaacc
    15181 cggcgaattt catcgacggt cagaaagcct ttattggtga cgccgctcgg tttgtagcca
    15241 tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag gcgcaagtag
    15301 taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt tccagatacg
    15361 ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat tgtcacggcc
    15421 aaagcaccat atcagttgac ccaggtgcag aggctagagt gtttagtggt taagccgata
    15481 ctagcgtgta aatgagattt ttaccatcca cactttcgct atgtaatcat gtatatagcg
    15541 tcatgatcgt gctatttcgc tacagaataa gggaaaatgg cggttttact ttgatataca
    15601 tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag tttgtatcct
    15661 caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc gcggataatc
    15721 tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc
    15781 tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt
    15841 taacgggtat cgtcaccacc acggataatg gtggctcgac ggggcgtatt cgccgttcag
    15901 aaggcggcat tgcctggggc gatatgcgca actgccttaa ccagctgata actgaaccca
    15961 gcgtcgcttc cgcaatgttt gaatatcgtt tcggcggtaa tggcgaactt tccgggcaca
    16021 accttggaaa cctgatgttg aaggcactgg atcaccttag cgtgcgacct cttgaagcga
    16081 tcaatctgat tcgaaatctg ctgaaagtgg acgcacattt gattccgatg tcggagcatc
    16141 ctgttgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg
    16201 accagttaac tacgccgatt caagagttat tgttaacgcc taatgtaccc gcaacgcgtg
    16261 aggcggttca cgctatcaat gaagcggatc tcatcattat tgggcctggc agtttttata
    16321 ccagcctgat gcccattctg ctgctgaagg aaatcgcgca ggcattacgc cgcacgccag
    16381 cgccgatggt ctatatcggc aatctggggc gtgagttgag tttacctgcg gccaatttga
    16441 agctggaaag caagctggca attatggagc agtatgttgg taaaaaggtc attgatgcgg
    16501 tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg attcaggagg
    16561 tgctggaggc cagcgatatt ccgtatcgtc atgatcgcca gttgttacat agcgcactgg
    16621 aaaaggcgtt acaggcttta ggttaacgga aatccgaagg aaaattccgg cttcctattg
    16681 aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    16741 ccgcgctaat tcaatgtatt gcagaatcat gtaggcctga taagcgtagc acatcaggca
    16801 attttgcttt tgtcatctgt cttcttcgtt atcctgtcac gaagccgcga taaacaattc
    16861 acgcagctga tgcaactggt cacgaatttg tgccgcttct tcgaactcca gattctgcgc
    16921 gtgttgcatc attaaccctt ccagctcatg gattttctgt tgcaacgctt taggcgacat
    16981 atccatcggc acattatccg gctcaacaat cgggcgcgat tttcctctgc ccttcgcttt
    17041 ggttttggcg atgttctgcc ccagcgccag gatatcgacc actttcttgt tcaagccttg
    17101 cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc tcgcggcgac gttcggtttc
    17161 gccaatcgct ttcgccattg atggggtgat cttatcgccg tagagaatcg ctttaccgtt
    17221 aacgttacgt gccgcacgac caatggtctg gatcaacgaa cgttcggaac gcaggaagcc
    17281 ttctttgtca gcgtcgagga tcgccaccag cgaaacctcc ggcatatcca gaccttcgcg
    17341 cagtaagttg atccccacca gcacgtcgaa ctcacccaga cgcaagtcgc ggataatctc
    17401 catacgttcg acggtgtcga tatccgagtg aagataacgc acgcgctcgc catgttcttc
    17461 gagatattcg gtgagatctt ccgccatccg cttggtcagt gtggtgacca gtacgcgttc
    17521 gttaattgcc gctcgctgac gaatctccga aagaagatca tcaacctgtg tcgccactgg
    17581 ccgcacttcg ataatcggat caagcaatcc ggttggacgc accacctgat ccaccacatc
    17641 gccgccggat ttttccagct cataattacc tggcgtcgcc gaaacataga tggtttgcgg
    17701 cgctaatgct tcgaactctt caaatttcag cggacggtta tccagcgctg atggcaggcg
    17761 gaagccgtac tccaccagtg tctctttacg cgcccggtca ccgcgataca tgccgccaat
    17821 ttgtggaatg gtgacgtgag attcatcgac caccagcagc ccatcggcag gcaggtaatc
    17881 aaacagcgtc ggcggtggct cacccggtcc acgaccagag aggaagcgcg agtagttttc
    17941 aatccccgaa cagtagccca gctcgttcat catctccaga tcaaactggg tacgctgggt
    18001 cagccgctgc tcttccagca gtttgttgtt ttccaacagc actttgcgtc tggcggccag
    18061 ctcttctttg atctcctcca tcgcctgtac gatgcgctcg cgcggtgtga cgtagtgcgt
    18121 tttcgggtag atggtaaaac gtggaatagt ggaaacaatc tgcccggtca gcgggtcaaa
    18181 taacgacaat cgttccactt cctcgtcaaa cagttccacg cgaagtgcaa tgtcatccga
    18241 ttctgccggg aatatatcaa tcacctcgcc acgaacgcgg aaagtaccgc gctggaatgc
    18301 ctgatcatta cgagcgtatt gcagctccgc cagtcggcgc agaatcgcgc gctgatcgat
    18361 aatcataccg accgtgagat ggagcatcat cttgagatat aaatcaggat cgcccagacc
    18421 ataaatcgcg gaaacagacg ccaccacaac cacatcacgt cgctccagca tcgctttggt
    18481 ggcggacaaa cgcatctgtt cgatatgttc gtttaccgag gcatctttct caatgaaggt
    18541 gtcagaactc ggtacatagg cttccggctg atagtagtcg tagtaggaga cgaaatattc
    18601 caccgcgttt tccgggaaga actctttcat ttcgccatac agctgggccg ccagcgtttt
    18661 gttgggcgca agtaccatgg tagggcgctg aaggtcagca atgacattgg caatggtgaa
    18721 ggttttcccg gagccagtca cgccaagtaa cgtctggtgc gccaggccat cttccagccc
    18781 ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa ggtttaaaag cggaattcag
    18841 tttgaacggt ttactcatga gtctctacct gaaggagttg ggcgggcagg tatgtaattt
    18901 tactcgtcgt acttaattat gccaacaaat tatactggat aaaaaaacag ttcatcacca
    18961 taatatttct gatacagcgt aaactccgct gtcaatcatg agcaaaattt actctgtggc
    19021 gagataaaac tccggcctta ccgggttatc cccaaagcaa cggctttttt aacatttgtc
    19081 aagatgagtg gtgacagttt tatggcaaga gatgcctcgt tagtgactgc cattgcaatt
    19141 atctaaccag ttaaaaaata aaagaaattt ctttgagccg tctttaacgc caattcgcgt
    19201 gcaagccgcg tattctctcg cttgcctcgt gttttctaac tctaatacac atggttatcc
    19261 acaggaatag tggataactg tctgcagccc ctatcccccg ccgcctgggc aattcccaca
    19321 ttcgccgcat gatgcggaag taaaattttt ttcgtgatta ttttagaatt taattggtta
    19381 aattgcagtc aatcgaagac gcgatctcgc tcgcaattta accaaacaca ggatggctac
    19441 aacaaggcaa ggtttatgta ctttccggtt gccgcatttt ctggattttc tgcaagcctg
    19501 gggatctctc ccagcagcgg cgcgggaatc atgcgggtga gcgtggtcat atattcagcg
    19561 tgacgttttc ccggaggcgt gacatcgttc gccacccaac ccgccagagt cagcccggcg
    19621 tgttgtatcg cctgtgccgt caacatcgcg tgattaatac agccaagttt cacgccaacc
    19681 accagtatta ccggcagttg ttcctgtgtt acccaatccg caaaagtgaa agtgtcagaa
    19741 agcggcgtaa accagccgcc agcgccttcc actaacaccc agtcagcctg ttgttcaagc
    19801 gcgcgtaatc cggagctcat taccgatgat tctatcgacc tgccctcttg cgcgctgatg
    19861 atgtgcggtg aggtgggttc tgcgaaggtg taaggattta ccgttgcgta atccagctgc
    19921 aggctgctgt tgcgctgtaa cgccagcgcg tcactattac gtaaaccctc cggggtcttt
    19981 tcgctgccag aggcgaccgg tttataacct gccgtccgat agcctgctgc ctttgcggct
    20041 tgtaaaagtg cacaactggc gacagttttc cccacttcgg tatccgttcc ggtgacaaaa
    20101 taacgtttac tcacgaacaa tcactcccaa aaaaagatga tacgtcagag gatatcgccc
    20161 ctgttgttgc ggccaggcca gttgcaatcg ctgcaactgt gaacgcgtta atattcgctg
    20221 gtcgcgccct tcatgaagat gcgtggcacc gatgcctttc agcgaacgca tggcactgag
    20281 cgcatcatca aaccacagcg tgatgggctg aatatgatgt cgataatgca cgccgttcag
    20341 tgactgttcg atttcatctg gcggtaaaaa gcgattagca tgcgggcgct cgtccaccgc
    20401 ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc agcgtggtaa acgcgaccac
    20461 gcctccgggg cgcaccaccc gatacagctc gcggagtgcc gtagataaat taccgcacca
    20521 ctgcaccgcg agattgctcc atacaagatc gaacgtcgca gtcgctaacg gcagggattc
    20581 gatatctccc gccagataat gatctgcggc atctttatgg cgggcttgcg caagcattgg
    20641 cggcgagaga tctaacgccg tcacctgcgc gtgacgctct cgccagtggc ggctcatcca
    20701 gcctggccca caccccgcgt ccagtacgtg ggtgtattta cgctgtggga gcattgccag
    20761 taagacgtca gcactctggc gctgtaactc tgcgtgttgc tcatagtgcg cggctgcccg
    20821 accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc atgcagcacc tccagcagac
    20881 ggtcgatatc ctgcatttca tgcgcggagg ttagtgttaa gcgcagtcgc gcagtaccag
    20941 cgggtacggt tggcgggcga atcgccgtga cccagcagcc ttgctggcgc agtttttctg
    21001 ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg ctggatggcg ctgcatgaat
    21061 cagcaagcgt aaacggcaaa tcctgtactc cggcacgaaa acgcgtaatg agtgccgcca
    21121 gtttttcgcg ccgtgcatca ccctcatcac tgcgaatgac cgccagtgac gcacgtaatg
    21181 cctgcgcctg agcgggcggc atactggtgc tgtagataag atggcgggcg aattgcagca
    21241 gataatccgc caccgtactg gagcaaagca ccgcagcccc gctgacgcca aatcctttgc
    21301 caaaagtcac taccagcaat tctggtttta ccttttgcaa ccagcaggtg ccgcgcccct
    21361 gctccccgat aacgcccgtg ccgtgggcat catcgaccat caaccagcca ttgtgctgtt
    21421 gcgttacctg ctggatttcc gccagtggcg cactatcgcc gtccatgctg aacacgcctt
    21481 ctgtcaccac cagttgctgc cccgaacagg gggaagcaag cagtcgcgcc agatgagcga
    21541 catcgttatg gataaaacgg cgaagctgcg acgggcttaa actggcagcc tccagcaatg
    21601 aggcatggct aagccggtcg gcaacaatac ggtcctcttt cgccatcatc gcggcaatga
    21661 ctgcctgatt cgcggcaaaa ccagagataa acagcagcgc ccgcgaatag ccaagccact
    21721 cggccagttc ttcttccagc gcctgatgcg ccacgctata accgctgaca tgcccggaac
    21781 cgccgctacc tacaccaaac ttttctgcgc cctgctgcca ggcgcggata atttgcggat
    21841 gatggcttaa acctaaataa tcgttactgg aaaagttcag atactggcga tcatccgcca
    21901 ccagccagcg tccggctcct tgcgtcaccg gataacggcg acgcagggca tcggcagcac
    21961 gccgcgcatc gagcgccgcg ttgattttct cctgccagct catagtgctg ccgcgttgta
    22021 atattcgtca gtatccgggg tcatcagtgc ctgctccaga cgctgctgtt gttcgttatc
    22081 ccctgccagc acggcagttt gctgcggatt taaccccagt ttgcgaaaca gttgcaggtc
    22141 tttatcttct tccggattcg gcgtagtcag cagtttgcag ccgtagaaaa tcgagtttgc
    22201 gccagccata aagcacatcg cctgagtctg ttcgttcatc tgctcgcgtc cggcagaaag
    22261 gcgtacgtaa gaggtcggca tcatgatccg cgctacggca atggtgcgaa taaaatcaaa
    22321 ggcatcaacg tcatcgttat cggcaagcgg cgtgcctttc accttcacca gcatgttgat
    22381 tggcacgctt tccggcggcg tcggcaggtt tgccagttgc agtaataatc cggcgcgatc
    22441 ttttaccgtt tcgcctaaac ccacaatgcc acctgagcag actttgatcc cggcatcgcg
    22501 cactttttcc agcgtatcaa ggcgttcctg ataagtacgt gtggtgatga tattgccgta
    22561 aaactccggc gaggtgtcga ggttatggtt gtagtaatcc agcccggcat tcgcaaggcg
    22621 ctgcgcttga gattcactca acgtgcccag cgtcatacac gcctccagcc ccatcgcttt
    22681 taccccctgc accatttgtt ccaggtacgg catatcgcgt tcgtggggat tcttccacgc
    22741 cgcgcccata cagaaacgcg tcgatcctgc cgctttcgct ttgcgcgccg actccagcac
    22801 ctgttcaact tccatcaacc tctcggcttc cagcccggtt ttgtagcgcg agctttgcgg
    22861 gcagtatttg caatcttccg gacaagctcc ggtcttaatc gacagcaacg tgctgacctg
    22921 cacctgacga ggatcgaaat gctggcgatg gacctgctgc gcttcaaaca gcagatccag
    22981 caacggtttt tcaaataatt ctgtgacttg cgacaatgtc cagcgtgggc ggtgagccat
    23041 ggggcttctc caaaacgtgt tttttgttgt taattcggtg tagacttgta aacctaaatc
    23101 ttttcaattt ggtttacaag tcgattatga caacggacga tcttgccttt gaccaacgcc
    23161 atatctggca cccatacaca tccatgacct ccccgttacc ggtttatccg gtggcaagcg
    23221 ccgaaggttg cgagctaatt ttgtctgatg gcagacgcct ggttgacggt atgtcgtcct
    23281 ggtgggcggc gatccacggc tacaatcacc cgcagcttaa tgcggcgatg aagacgcaaa
    23341 ttgatgccat gtcgcatgtg atgtttggcg gtatcactca tgcgccagcc attgagctgt
    23401 gccgcaaact ggtggcgatg acgccgcaac cgctggagtg cgtttttctc gcggactccg
    23461 gttccgtagc ggtggaagtg gcgatgaaaa tggcgttgca gtactggcaa gccaaaggcg
    23521 aagcgcgcca gcgttttctg accttccgca atggttatca tggcgatacc tttggcgcga
    23581 tgtcggtgtg cgatccggac aactcaatgc acagcctgtg gaaaggctat ctgccagaaa
    23641 acttgtttgc tcccgccccg caaagccgca tggatggtga atgggatgag cgcgatatgg
    23701 tgggctttgc ccgcctgatg gcggcgcatc gtcatgaaat cgcagcggtg atcattgagc
    23761 cgattgtcca gggcgcaggc gggatgcgca tgtaccatcc ggaatggtta aaacgaatcc
    23821 gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga cgagatcgcc accggatttg
    23881 gtcgtaccgg caaactgttt gcctgtgaat atgcagaaat cgcgccggac attttgtgcc
    23941 tcggtaaagc cttaaccggc ggcacaatga ccctttccgc cacactcacc acgcgcgagg
    24001 ttgcagaaac catcagtaac ggcgaagccg gctgctttat gcatgggcca acttttatgg
    24061 gcaatccgct ggcctgcgca gcagcaaacg ccagcctggc gattctcgaa tctggcgact
    24121 ggcagcatca ggtagcggct attgaagcgc agctgcgcga gcaacttgcc cccgcccgtg
    24181 atgccgaaat ggttgccgat gtgcgcgtac tgggggccat tggcgtggtc gaaaccactc
    24241 gcccggtgaa tatggcggcg ctgcaaaaat tctttgtcga acagggtgtc tggatccggc
    24301 cttttggcaa actgatttac ctgatgccgc cctatattat tcacccgcaa cagttgcagc
    24361 gtctgaccgc aggggttaat cgtgcggtac aggatgaaac atttttttgc caataacggg
    24421 aagtcagcgt gagggtttct ggctacactt tctgcaaact agaaaggagg gtttatgaaa
    24481 ctcatcagta acgatctgcg cgatggcgat aagttgccgc atcgtcatgt ctttaacggc
    24541 atgggttacg atggcgataa tatttcaccg catctggcgt gggatgatgt tcctgcggga
    24601 acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc caaccggctc cggctggtgg
    24661 cactgggtag ttgttaattt acccgctgat acccgcgtat taccgcaagg gtttggctct
    24721 ggtctggtag caatgccaga cggcgttttg cagacgcgta ccgattttgg taaaaccgga
    24781 tacgatggcg cagcgccgcc gaaaggcgaa acccatcgct acatttttac cgttcacgcg
    24841 ctggatgtag agcgtattga tgtcgatgaa ggagccagcg gcgcgatggt cgggtttaac
    24901 gttcatttcc actctctggc aagcgcatcg attaccgcga tgttcagtta attactctgc
    24961 cagatggcgc aatgctatct ggtatcactt aaaggtatta aaaacaactt tttgtctttt
    25021 taccttcccg tttcgctcaa gttagtataa aaaagcaggc ttcaacggat tcatttttct
    25081 atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg cgctggcatt
    25141 ggcttttggc gtgacgctga ccgcctgtag ctcaacacca cccgatcaac gtccttctga
    25201 tcaaactgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag cgcagaattt
    25261 cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga atccgtcccc
    25321 gataaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggtactc aaggtgtaac
    25381 gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgcacca acaagcgcca
    25441 gtatattgcc gtgatgcctg gtgagtatca gggaacggtg tatgtccctg ctgctccagg
    25501 tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg ggctttccct
    25561 tgatgggggc atgagtcctg ccgactggcg tcacgacgtc aacccgcgcg gcaaatatat
    25621 gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca gcgacagtat
    25681 cggtgttctg tgttctgcgg tcttctggtc acaaaacaat ggcctgcaac tgcaaaacct
    25741 gaccatcgaa aacacgctgg gtgatagcgt agatgcgggt aaccatccgg cggtggcact
    25801 gcgtactgac ggtgaccagg tacagattaa caacgttaac attctcggtc gtcagaacac
    25861 cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc agccgcgtac
    25921 gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc gcggcgcagt
    25981 ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag aagcgtatgt
    26041 gtttgcaccg gctacgctgt ccaacattta ctacggtttc ctcgccgtaa acagccgttt
    26101 caatgcttcc ggtgatggcg tggcacaact gggccgctcg ctggatgttg atgccaatac
    26161 caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca cggctaaacc
    26221 gtggggcgat gcggtgatct ctaatcgtcc gtttgcgggt aacaccggca gcgttgacga
    26281 tagcgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt gggaatacaa
    26341 taaccgcggc gtgggtagca aagtggttgc agaggcgaag aagtaagagc tattaactat
    26401 ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt tgtaggtcgg
    26461 ataagatgcg ccagcatcgc atccgacaat aagtgccgaa tgcgacctac attcacatgg
    26521 cgctttttac atctgacggt ttttattgaa gttaatcaaa ctacccgcct tgataatctc
    26581 gcgctcttca gcagtcagac tttccatata gagcgtaatt tccgttaccg gcgcatcttc
    26641 atggatcaca taacctttaa acgtcgtacc cggattatcc agcgccgctt taatgccagg
    26701 gatgtaaatg taatccccca cttcaaaggt tggcacttcc gccatctgca gaggtaacat
    26761 cccccagttg atgacgttag aacgatagcg tttagtcgcg tactcctcgg caatattcgc
    26821 cagaccgcca atcacacgct gacaactcgc cgcctgttca cgcgcagaac catcgcctgg
    26881 tttcaccgca tagaccatgc tgccaatttc agtttgcagc ggatcaatat gctcctgacc
    26941 agcaatctgc ttaatgcgcg caaacacctc tgtcagctcg ctgacattcc ccgccagacg
    27001 ctgattttcc agctcagcag tcgctttact tctgccaaca taacctggat cgcggcgtga
    27061 cagggtaaac tccgccagac caatcggatt tgaacgataa gaagaggttt caccggaagg
    27121 gatcagttcg tcggtggtgg tcacttcgtc gaggatcttc gagcacactt tgaggacgat
    27181 attgtcagtc agcgcaccta attccggcca gtctttaatg ttcggtccgt aaatcagcgg
    27241 ttgctgagtt gcccctttaa caaagccctg ataaacacgg tttttatacg gcgttacatc
    27301 gaaggcgtac tccggcacgt tgtcccagca atcgagttcg ctggcagaag ttaaatagcc
    27361 accgtttgcc gcagtcgcag cgatagaacg agcgtccatc aacgccaccg ctgacatctg
    27421 cccattggct ggcttagaac cttcgcggtt cgggaagtta cgcgtggtgt ggcgaatact
    27481 caaaccgttg ttgattggcg tatcgcccgc gccaaagcat gggccgcaga acgcggttct
    27541 gatgattgcg cctgcgccaa tcaaatctgc taccacacct tttttggcta aatccataaa
    27601 tactggctgt gatgacgggt aaaccgctaa cgagaaggtg tcattgccac aggattgacc
    27661 gcgcagtgca ttcgccgccg cgatgacgtt ttcgtagtta ccgccagaac agcccgcgat
    27721 aatcccctgc tgcactttca ggcgaccatt ttccactttg tccagcagcg agagtttggc
    27781 tttaccgtgc gccacgcgtt cggactcaat ttcaatctca cgcagaatgt cggtcaggtt
    27841 ctggttcagt gtgtcgattt catacacgtt gctcgggtgg aacggcagcg caatcattgg
    27901 tttgatggag cttaaatcaa cgctgatgca gccatcgtag tacgccatcg gttgagggtt
    27961 aagctggcag taatcctggc cgcgaccgtg cagcgccagc cagttatgga cttcttcatc
    28021 ggtttgccag acagaactta aacaggtcgt ttcagtggtc ataacgtcaa cgctgttacg
    28081 gaaatcggta gagagcgcag caacaccggg acctacgaat tccatcactt tgtttttgac
    28141 gtagccgttt ttgaacacgg caccgatgat agccagcgcc acatcctgcg gccccacata
    28201 cggcgctggt tttccggtca gatgcaccgc aacaactccc ggatagtcga tatcccaggt
    28261 gtcattaagc aattgtttta ccaactcacc gccgccctca ccgactgcca ttgtccctaa
    28321 tgcaccgtaa cgggtgtggc tgtctgaccc gaggatcatt ttgccgccgc ctgccatcat
    28381 ctcacgcata tattgatgga tgaccgcaat atgcggaggc acaaaaatac cgccataacg
    28441 ctgggccgcc gataaaccaa aaacatggtc atcaccgtta atagtaccgc cgacggcgca
    28501 gagtgagtta tggcagttgg tcagcacata cggcagcggg aaacgttcca ttccggacgc
    28561 tttagcggtc tgtacaatac caacaaaggt aatatcgtga gaggctaatg aatcaaactt
    28621 aattttaagt ttatccatat ttccggacgt attatgagaa gagagaatag accaggcaat
    28681 agtgcctttt tttgcttctt cttttttaat ttcgccggtg aaatgttctt cggcaattat
    28741 ttcgttatta ctggcgagaa acacgccttt ttcagataac ttgatcatca aatgctccag
    28801 atatctgcgc accgtgaagt gcgcagaaag ataataaatg gatatactct aaataattcg
    28861 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    28921 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat ttacagcatg
    28981 ttccagccga tcagcatatt ccaccaccac acgccgaggg tgatatgcac caggaaggtt
    29041 aatatcgtca gtaccgcacc gaccaaccac caggatttaa tatcgttgta acccacgcca
    29101 aagatgaccg gacctgccgc gccgccatag tgagtaacca tgccgccata ggagttggag
    29161 aacaacagtg ccagcgcggt taacattaac ggtgcgccgg agacgttcgc cagcatggca
    29221 aatacaggta acatagcaac gatataggca ctaccggaag cgaagaaata acgcacgatg
    29281 atgctgagga aaataataac gaagaaagca acgttaccgt gaccatcaaa tgccaggtta
    29341 tttttaaaga cttcagctaa ccattcgaag aatttaactt tcgataataa ggagcttaag
    29401 ccgataatac cgccgtacca aattaaagta ttccagccgc ctttattttt aaccacgtct
    29461 tcccaggtaa caatacccag cagcagcatg gttgccataa caacgattgc cacggtggat
    29521 tcatcaaccc ccagagactt actgaaaatc cagcccagca gcgccagcac aaagacaccg
    29581 agcagcattt tttcgcggat tttcatcggt cccagttcgg caaggcccgc tttagcgatg
    29641 gttttgttat ccaccttctt aatttctggc ggatacatgg tgtaaatcac cagcggagta
    29701 accagcagca taatgatgcc cggcaatccg gcggccagcg cccatccccc ccagctaatt
    29761 tgcaggtgca gaatgtcgtt gatcattttc agcgccagaa tgttccccgc cattgcggta
    29821 aagaacatat agctggtggt tttggtgacc atgtaaatgg acatcatcag gtaatgtccg
    29881 acacgacgcg gacttttttc cggttcggac cccaaagcca ccgccacgct gttgatgatc
    29941 ggtaacacaa tgccgcccgc acgcgcggtg ttagacggtg ttgccggagc cagtaccaga
    30001 tcgaggaaaa ccgtaacgta acccagaccc agcgtggtgt taccgatttt accaatcagc
    30061 agataggcaa tacgtttacc taaaccggtg gtcacaaatg cggcgcttaa ggtaaacgcc
    30121 gagaacacca gccaggtagt acctgaagag taaccgctta atacggcggt ggttttaaac
    30181 gcaccgtcgg ataagttacc gaccaccacc atggaggcag caacggcaat taacagtacg
    30241 acaggttccg ggaaaggctt gataaccagc cccacaatgg ccgctaggta aataccaaaa
    30301 agcacccacg ccagttcgct taatcctgcc ggagctggca taaagccaat aatacatggg
    30361 atcgctaata tcagaattag cttccataac gatttcttat tcatttaaaa tcctgttctt
    30421 tctgtatgac tcatacagaa gtcctttatt gattgtcctt atcctgacaa cgaatctttt
    30481 cagggaagat aaacttcacc ggaaaatatt tttctggtcg tccgaataac agatgcgcgt
    30541 aacgtcgtgg catcctgacc ttcattactt aaatgaacgt cgagcgcacc actggggtgt
    30601 tcaatattga tattgccata tcctacagaa gggatgattt gtcgggtgac ggtgccttcc
    30661 aatgcacaac tactggaaat agcaatagca ccggttatcg ccaacgcgcg atggcaagaa
    30721 tgcggcataa aataacgcac attaattgcc ccgcctttct gcgctggaga aataagcaca
    30781 ggtttaggga taaccatatt actgacatca cctaagccca ttgctttacc cgcttgtaga
    30841 cggatagatt caatgcgggc taataatgct ttgtcggcat ccagttccgc cggtaattca
    30901 tagcctgttt ttcccagata ttcagccgga ataatgacga ctggcatcgc catatcgata
    30961 caggtcaccg ggacatcgtc aaaataatca atctgattat cagtcgggaa aacttttccg
    31021 gttttggttc cagcggcatt caggaaagtg agcgcaaccg gtgcggcagt acccggtacg
    31081 ccgtcaattc tggcactacc ctcgtactcg acaacaccat ttggcgtttg cacatcagct
    31141 tcgatgaacg tacccgtatt gacgttgcgg atacgtacgc gggtaactgg cgaagtcgct
    31201 gcaatcaaac cattttcaat ggcgaatgcc ccaacgccag acagcatatt gccgcagtta
    31261 ggcgtggtat cgacacgttg ctcatggacg ataacttgag caaacagata atcaacatca
    31321 gcacgcggat cgctggaacg gctaataatg gcgactttac tggtcagtgg attaccgccg
    31381 ccaataccgt caatttccag atcgttaccg gaacccataa ttgccatcaa tattttatcg
    31441 cgctgcgttt gatcttcggg taaatgttcc gctaacagga acgcgcccct cgaggttcca
    31501 cctcgcatca tcacgcaggg tatttttttc ataataatgc cctcaattta aaaacataac
    31561 ttattgcgat atggttacat taagggcaaa gcatctctaa tgactttagc tcaacgatta
    31621 ataacatttt ttcattaatt taagttggct aaatgcagtt aaaattttta acggccagcc
    31681 acccaaaatc gtcgcttcca tttcccggag taataatgaa gcatgaatta tcaagtatga
    31741 aggcatttgt catactggca gagtccaatt catttaacaa tgccgctaaa ttactcaata
    31801 ttacgcaacc tgcattaaca cgaagaataa aaaagatgga ggaagattta catattcaac
    31861 tatttgagcg tacaactcgc aaggttacgt taacaaaagc aggaaaaagg ttgctcccag
    31921 aggcgcggga attaataaag aaattcgatg aaacgctttt taatattcgt gatatgaatg
    31981 cttatcatcg tggtatggtg acgttagcgt gtattccgac cgcagtattt tattttttac
    32041 cgctggcaat cggtaaattt aatgagctgt atcccaatat taaagtgcgg attctggaac
    32101 aaggcacgaa taattgcatg gaatcggtgc tatgtaacga atcagacttt ggcataaaca
    32161 tgaacaacgt cacaaattca tccattgatt ttagcccact ggtcaacgag ccgttcgtgc
    32221 tggcctgtcg gcgtgaccat ccattggcca aaaagcagct tgtagaatgg caggagttag
    32281 tgggttacaa aatgattggc gtacgctcct ccagcggcaa ccgactgttg atagaacaac
    32341 agctagccga caagccctgg aagctggact ggttttacga agtgcgtcat ctttcgacgt
    32401 cattaggact ggtggaggca ggactgggga tttcagcgct tcctggtctg gcaatgcccc
    32461 atgcgcccta ttcttcgatt attggtatcc cactggtgga acccgttata cgtcggacat
    32521 tggggattat tcgccgcaaa gatgccgtgc tttctccggc agctgaacgc ttttttgcct
    32581 tgctgattaa tttgtggact gacgataaag acaatttgtg gaccaatatc gtcgaacgcc
    32641 agagacatgc gttacaggag ataggttaat acaccggcgc aggagagact gcgccgggta
    32701 aatcagcggt tagtgtgcgt taaccaccac ccacatcggt ccctgcccga ccgcatagcg
    32761 gcctttttca tgcagtagcc cctgctcacc aacaatttcg tataccgaga tatggtgaga
    32821 tttttgcccg gcggcaatca gatacttacc acggtgatcg atattaaagc cgcgcggctg
    32881 ggtttcagtt ggctggaagc cctctttact caacacgctg ccatcttccg aaacgctgaa
    32941 gacagtaatc aggctggcgg tacggtcaca ggcatataag tgacgaccat ccggggtaat
    33001 atgaatatca gccgcccaac gggtgtcgga gaagttttcc ggcatcatat ccagcgtctg
    33061 gacacattcg atattgccgt gcggatcttt cagttcccag acatccactg agctgtttaa
    33121 ctcattgacg caatacgcat attgttcgtt tggatggaat accatatgac gcgggccggc
    33181 cccttcaacg gtagtcactt ccgcagggtc ctgcgccacc agatgaccat catcgctgac
    33241 cgtaaacagg caaatgcgat cctgctttaa tgccggaacc cacagcgtac ggttgtccgg
    33301 tgagatattg gcggaatggc aaccgtccag cccctcgacc acatcgacga cgccaactgg
    33361 caggccatct tccagacgcg ttacgctcac gttacccgca ttgtaagaac ctacaaagac
    33421 aaactgcccc tggtgatcgg tggaaatatg cgtcggacta cccggcagcg cagactctgc
    33481 ggcaaaggtc agtgcgccat cgtccggggc gatacgatac gccaggacgc gaaactcagg
    33541 gcgaacacca acatagagat aacgtttgtc cgggctgacc accatcggct gcacctgccc
    33601 cggcacatcg acaacctgtg tcagcgtcag tgcgccttca tgattcaggt tccagacgtg
    33661 aatttgctgg ctctcagggc tggcgatata aactgtttgc ttcatgaatg ctcctttgca
    33721 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
    33781 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagaaga aaattcttaa
    33841 tcttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
    33901 attgaccccg aaaaagaccc tgctaccttc atcgatagaa gccctggccc gcgctcgcga
    33961 agcaggttat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
    34021 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
    34081 tcatgcaaaa actgtgcttg aagccgatcc aatgcctgtt aacaaagccc tgcaactcat
    34141 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgacg cgatggttta
    34201 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
    34261 acagcgtccg actttcacac aagtcgcttc tctggctgaa acggcgcaac aagttaacgc
    34321 cgtatggaag ttcgccctca cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
    34381 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
    34441 acgcggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggctggtc
    34501 gatggaaaac gtcgtggcgt tcggcgataa ctttaatgat atcagtatgc tggaggccgc
    34561 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
    34621 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
    34681 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
    34741 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acctccagtt
    34801 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
    34861 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
    34921 tacgtagcgc agcttgcagc ggttcgtcca gcttattgac ccacaaatgc tgatcgccca
    34981 gcgccagcgc agtcatcgcg taatgcggat gatgctcaag taccgtcact ttcagaatgc
    35041 tactttgttg ctctttcggc agccacggat tcatcacgct actaccccag acttcttcca
    35101 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
    35161 ggatctcatc cagcgaatgg ctgacataca acatcgggat gttgatttcc cgcgtcagac
    35221 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
    35281 ccagcaacag caattctggt gccgtcagca aagcccgccc aatcgctacg cgctgttttt
    35341 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
    35401 ccagcttatc gaactgatcg accatgcttt tcgccatgcc gtagcgcaga ttgccacgca
    35461 ctttgtaatg cgggaacagc cgcgcatcct gaaaaacata gccaacgcga cgcttttccg
    35521 gcgtcaggca gatacctttc tcggcatcat ttagtacccg accattgagg acaatcctcc
    35581 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
    35641 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaata gtcaggcaat
    35701 ggttgcccaa cgtctgggaa aaattcagtt ccagcatgat tagcgccccg cccgttcacg
    35761 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
    35821 aatgcacagt ctcgctgcgc cactttcccc gccgggggtc tgaatcaggg tatacatggc
    35881 agaaggaatg gttcgcgttt caccaggaat gttcgacaca aaggtgatgg ttgcaccaaa
    35941 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataatgcccg gtaatgtcag
    36001 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cccagtgttc ttgcggcctg
    36061 ttccagtttg acatcaactc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
    36121 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taatgccaaa
    36181 ccagtcatac aaacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
    36241 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
    36301 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
    36361 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
    36421 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgcagc ctgcggtccc
    36481 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
    36541 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
    36601 ctggcaactg cgtcagaacc gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac
    36661 gccagcgcgc cacgaacatc ttccgccgga gccagtttcg gggagagcgt atcccatgcg
    36721 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
    36781 accgccaggc gaccgccatt cagcagtgaa gtccagttgg ttttgctgtc gatggtaaaa
    36841 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
    36901 cgcgtagctg tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
    36961 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
    37021 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
    37081 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
    37141 ttgccagcaa cagcgaaaga gagtgccgcc ccagcaaaca agttcaacca tttacgagcc
    37201 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
    37261 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
    37321 aggaagttga gaaaaagaaa atgcccgact aagcgggcat tcagggaatc aatgattttg
    37381 tccaggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
    37441 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
    37501 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
    37561 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
    37621 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
    37681 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
    37741 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
    37801 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
    37861 gtggcggcgg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
    37921 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
    37981 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
    38041 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
    38101 ctgatggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgcg cgtctggggc
    38161 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
    38221 acgaagcggt cgcgcaaaac gctgacaacc aattaccggg tattattagt catattgagc
    38281 gcggcgcaga gcagtgcgaa gtattaatgg cgctaccgga cgggcaaaca ctgtgcgcca
    38341 cagtgccggt aaatgaagcg acttctcttc agcaaggaca gaatgtcacg gcctacttta
    38401 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
    38461 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
    38521 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
    38581 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc
    38641 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
    38701 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcagcggaat
    38761 aacaccgata tgctcggccc tggcgaagat gacaccggac gcactacggc tgagattatt
    38821 caggatgaag taaaggatgc accgcgctgc atgcaactgg cacagcagtt cggtattacc
    38881 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
    38941 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
    39001 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
    39061 actctggtac tggtgctcaa tcgcttcgat gagatcccgg agtttgtcca gtttgctggc
    39121 gtgctggcgg attgcacgtt agcggaaact ggcgctaaag aggaactgct ccaacaagca
    39181 ctcgtcgcgc aactggcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
    39241 gaaccttcag cacgtcacgc cttacccacc aacgaaccgc gcattgtgct gaataatggc
    39301 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatcctggc
    39361 gagcactggc aaattgtcgg gccaaatggt gcgggaaaat cgacgttatt aagcctgatt
    39421 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggacg acgtcgtggc
    39481 agcggcgaaa ccatctggga tatcaaaaag catatcggtt acgtcagcag tagtttgcat
    39541 ctggattacc gggtcagtac tactgtgcgt aatgtgattc tttctggcta ttttgattcg
    39601 attggcattt atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
    39661 attctcggca ttgataaacg cacggctgac gctccgttcc atagtctttc ctggggacag
    39721 cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgac
    39781 gaaccattgc aggggcttga tccgctgaat cgccagctta ttcgccgttt tgttgatgtg
    39841 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accatgctga agatgcgcca
    39901 gcttgtatta cccatcgtct ggagttcgtg ccggacggtg gcctctatca ttatgcgctg
    39961 acaaaaataa actaagtcgg taatgctgac tgtgccggat gcggcgtaaa cgctttatcc
    40021 ggcacacggt cagacgcatc caggcatgaa actgcgtctt tttttcagat aaaaagcgca
    40081 atcattcaca atccctctgt tttataatca cttaatcgcg cataaaaaac ggctaaattc
    40141 ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt tgttatgcta
    40201 tggttatttc ataccataag cctaatggag cgaattatga gagttctggt caccggtggt
    40261 agcggttaca ttggaagtca tacctgtgtg caattactgc aaaacggtca tgatgtcatc
    40321 attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc
    40381 ggcaaacatc cgatgtttgt tgaaggcgat atccgtaacg aagcgttgat gaccgagatc
    40441 ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc cgttggcgaa
    40501 tcggtacaaa aaccgctgga atattacgac aacaatgtca acggtactct gcgcttgatt
    40561 agcgccatgt gcgccgctaa cgtcaaaaac tttattttta gctcctccgc caccgtttat
    40621 ggcgatcagc ccaaaattcc atacgttgaa agcttcccga ccggcacacc gcaaagccct
    40681 tacggcaaaa gcaagctgat ggtggaacag atcctcaccg atctgcaaaa agcccagccg
    40741 gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat
    40801 atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc ccaggttgct
    40861 gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga agacggtact
    40921 ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa
    40981 aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt aggcagcagc
    41041 gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa ctatcatttt
    41101 gcaccgcgtc gcgagggcga ccttccggcc tactgggcgg acgccagcaa agccgaccgt
    41161 gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac ctggcactgg
    41221 cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca atttaatccc
    41281 gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct ggtttcaccg
    41341 caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca ggtgttacct
    41401 gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac
    41461 cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat gtctgacacg
    41521 ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc
    41581 cgggtgatct gcttttcacc ggatcacagt aaaacgctgc cagagctcag tgttgcggca
    41641 ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag aactggggaa aacgtaccca
    41701 tgggtgcagg tctttgaaaa taaaggcgcg gcgatgggct gctctaaccc gcatccgcac
    41761 ggacaggttt gggcaaatag cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa
    41821 aaagaatatt ttgccgagca gaaatcacca atgctggtgg attatgttca gcgcgagctg
    41881 gcagacggta gccgtaccgt tgttgaaacc gaacactggt tagccgtagt gccttactgg
    41941 gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttacg gatcaccgat
    42001 ttgaccgacg cccagcgcag cgatctggcg ctggcgttga aaaagctgac cagtcgttat
    42061 gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc gccatttaat
    42121 ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct gttgcgctcc
    42181 gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg
    42241 accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc
    42301 ggagtgtaag aaatgagtct gaaagaaaaa acgcaatctc tgtttgccaa cgcatttggc
    42361 taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacc
    42421 gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagttgt
    42481 gcgccacgcg atgaccgtaa agttcgcgta atggcagccg attatgaaaa tcagctcgac
    42541 gagttttccc tcgatgcgcc cattgtcgcg catgaaaact atcaatgggc gaactacgtt
    42601 cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggcgt ggacatggtg
    42661 atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg
    42721 gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca aatcgcgctt
    42781 aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat ggatcagcta
    42841 atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgtcgctc actggggacc
    42901 aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt
    42961 accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc
    43021 ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt tgcacatgag
    43081 ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc ccgcaccgtt
    43141 gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt gatggcggag
    43201 tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga cactctggta
    43261 gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt
    43321 ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc
    43381 gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg taaaccatca
    43441 caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt cagccgtacc
    43501 gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga
    43561 ctttactttc agcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct
    43621 gtgccagccc ggaatgctat caggatcagg ccgcgtttct gggggcctct attggtcgtt
    43681 atgccaaccg catcgccaat agccgttata cctttgacgg tgaaaccgtg acgctttcgc
    43741 caagtcaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc
    43801 agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gacttcagat gatggcgatc
    43861 agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac gataaccgta
    43921 tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg
    43981 tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg
    44041 cggacgaata tctgccggtt gatgaaggcg gcattccgca cgacggcctg aaatctgtcg
    44101 ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg
    44161 acgatcagcg caaagtaaaa ggttacgatc acgcgttctt gttacaggcc aaaggcgatg
    44221 gcaagaaagt ggcggcgcat ctctggtcag cagatgaaaa attgcagctg aaggtctaca
    44281 ccactgctcc ggctctgcaa ttttactccg gcaatttcct cggcggcaca ccgtcgcggg
    44341 gaaccgaacc ttacgccgac tggcaagggc tggctctgga aagcgagttt ctgccggaca
    44401 gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcatcctggt gaagagtatt
    44461 ccagcctgac ggaatatcag tttattgctg agtaagttta agccctcgtc atgagggctt
    44521 tatctcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    44581 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    44641 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg
    44701 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    44761 tcaccggttg gtacgacgtg gatctttctg agaaaggcgt aagcgaagca aaagcagcag
    44821 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    44881 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    44941 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    45001 aaaccgccga aaagtatggc gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga
    45061 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    45121 aactgagcga gaaagagctg ccgctgactg aaagcctggc gctgaccatt gaccgcgtga
    45181 tcccttactg gaatgaaact attctaccgc gtatgaagag cggtgagcgc gtaatcatcg
    45241 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    45301 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    45361 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcagcg aaagcagcgg
    45421 cggttgctaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaatgag aaagccgact
    45481 gcaagtgagt cggctttttt gttgctaaca atggagcaca ttgcctgatg cggcgctgcg
    45541 cgtcttatca ggcctacggt gattcatcgg atacgccact ctggcggtgc atccgacaat
    45601 taaaccttac ccgcgacgcg cttttactgc attcgccagt tgacgtaaca gagcatcggt
    45661 atcttcccag ccaatgcagg catcagtgat gctcttaccg taggccagcg gctccccgct
    45721 ctccaggctc tgattgcctt caaccagatg actttccacc atcacgccaa taatggcctt
    45781 ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct ttttgaattg
    45841 ttttgacgag ttagcatggc tgaaatcgat catcacctgt gctggcaggc ctgctttgtt
    45901 cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt taccgccgcg
    45961 cagaatgata tggcaatcgc cgttaccgct ggtattcaca atcgccgaat gcccccattt
    46021 cgttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga tagccacttt
    46081 aatcgtaccg tcggtgccat ttttgaagcc gaccggacaa gaaagccctg atgccagttc
    46141 gcggtgcacc tgcgattcgg tggtacgtgc gccaattgcg ccccagctca tcaggtcagc
    46201 gagatattgt ggggtgatca tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt
    46261 aatatcaagc agcaatttac gggcaatacg caaaccgtcg ttgatctgga agctattgtc
    46321 catatgcgga tcgttaatca gccctttcca gcccaccgtg gtgcgcggct tttcaaaata
    46381 gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca gcaagcgagt
    46441 ggcgtactct ttagccgcta caggatcatg aattgagcac ggaccaatca ccactaacag
    46501 gcgatcatca ttccctttca ggattttatg gatcgctttt cgggcatggg caaccgtatt
    46561 agcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa gtaactcttt
    46621 gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag tgttgccata
    46681 cttatcttag tgaatgcaat acctccaatc tatatcttcc gtcagaatgt gtaaacgggg
    46741 ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa
    46801 aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat tatttcaaga
    46861 ttctctgctg gatttctttg cctggctgaa atcttaaacc aatgccttat attcacctgc
    46921 aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca tcctatgaaa
    46981 atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag cggtgccgca
    47041 ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc cgctgatgcg
    47101 ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg
    47161 gtaaatacgg gttctggcgg cacaatgctg catccggatg gttcttcaat gaacaatgac
    47221 ggcatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg ctgtccggat
    47281 atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac caaaaccgac
    47341 ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc tctccctttt
    47401 aatttttccc ttctaaaagc agtatgatgt cataaagtta agtgagaata attaagggat
    47461 gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa gataataatg
    47521 ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta gaagtcattg
    47581 gtggttttct ttctggttct ctggctttgc tggccgatgc gggtcatatg ttgaccgata
    47641 ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct cccaccattc
    47701 gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat gccatcgccc
    47761 tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc acgccgcgtc
    47821 cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gttgctggca aatatacttt
    47881 ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga gcagcggcac
    47941 tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg ttgattatta
    48001 tctggaccgg ctggacacct gctgacccca ttctctcgat actggtgtcg cttctggttc
    48061 tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgag ggtgcaccgg
    48121 tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca
    48181 atgtgcacca tgtgcatgta tggatggtag gcgagaagcc ggtgatgacg ttgcatgtgc
    48241 aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac tacctgatgg
    48301 atcactatca gattgagcac gccaccattc agatggaata tcaaccttgt catgggccag
    48361 actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa tgggacagcg
    48421 cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg agcgcaataa
    48481 aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca aaaataacga
    48541 cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta cgcgtcatca
    48601 gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg aaagcgtccg
    48661 gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatgatg actgccacgc
    48721 gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaata gcaatgacac
    48781 aaaccgccag ccaggttaat gccttcggca atggtaacca gcgaattttc aactccgcct
    48841 cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg gcaaagaaaa
    48901 acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg ccaaacaagg
    48961 tgacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca aggccaatac
    49021 acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa ccgcctgccc
    49081 ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt cccccaaagc
    49141 gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga
    49201 ttgagcggca ccagcgccct ctctcgcagc ctctcatcaa catgaacctc gtgattactt
    49261 ccttcctgtt ctaatgcctc agcgatagcc tgaaggccat tcatggccat ccacggacaa
    49321 tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg gtgcttccag taactcttta
    49381 tctggcaccg cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc
    49441 tgatgtggca atgtttttgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc
    49501 atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct
    49561 tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaatgca agcaccctgc
    49621 cagcataaaa tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt
    49681 gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac ggcaatgctt
    49741 gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg
    49801 acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga
    49861 tccagtgaac actcagcctg aagtgtcggc atcagaattg ttttttccgg actgagaatt
    49921 ttggcggttt ctcccataaa tctcacccca gcgactaaca acgtagaagc gggatgcttt
    49981 gcaccgaagc gcgccatttc caaagaatca gaaatacagc caccggtttc ttctgccagt
    50041 tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc
    50101 agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc
    50161 ggggggaaag gataaatcgc cgtgtctgga tcaaacatca cgctcatctt accatctcgt
    50221 tttacaggct taacgttaaa accgacatta gcgtgatatt cgctgttatc ggtgtaattt
    50281 gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgtatga
    50341 gatgccgaat gccacacttt gcgtcttatt cctccgacgt aaagttgttt acgcagcaag
    50401 cgcggagtat gtcatcaggg tcaagaattg aaattgagag atgcctgaat agcaaaaaag
    50461 cgcctttagg gcgctttttt acattggtgg gtcgtgcagg atgactcgct tcgctcgccc
    50521 ttcgggccgt cgccgcgagc ggctccgttg tttcgctgcg ctcaactcga acctgctgca
    50581 ggttcgaatc tttgagtatc gcagatagca aaaaagcgcc tttagggcgc ttttttacat
    50641 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
    50701 actgagctaa cgaccccttg cgggattatg ctgctttaat cattcaggat ggtgggtcgt
    50761 gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac
    50821 gacccatacg ggtgttacct gaaagatttt tactcggggc caatctttaa gattggtggg
    50881 tcgtgcagga tgactcgctt cgctcgccct acgggccgtt gctggcgcaa cgttatcctt
    50941 cacgcttaac atctgagttt gatgttaaaa ctggtgggtc gtgcaggatt cgaacctgcg
    51001 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccgag tggtgggtga
    51061 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
    51121 tcacccgata ctgcgctgga tactacaaac tacttccacc gaacccaccg taattagtgg
    51181 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc t
//
LOCUS       DAZGCQ010000025        51062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000025 DAZGCQ010000000
VERSION     DAZGCQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.025"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..795
                     /gene="elfA"
                     /locus_tag="ACHK07_001556"
     CDS             256..795
                     /gene="elfA"
                     /locus_tag="ACHK07_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101903.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            878..1579
                     /gene="elfD"
                     /locus_tag="ACHK07_001557"
     CDS             878..1579
                     /gene="elfD"
                     /locus_tag="ACHK07_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8101904.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1604..4204
                     /gene="elfC"
                     /locus_tag="ACHK07_001558"
                     /pseudo
     CDS             1604..4204
                     /gene="elfC"
                     /locus_tag="ACHK07_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4195..5265
                     /gene="elfG"
                     /locus_tag="ACHK07_001559"
     CDS             4195..5265
                     /gene="elfG"
                     /locus_tag="ACHK07_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101905.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5277..5819
                     /gene="ycbU"
                     /locus_tag="ACHK07_001560"
     CDS             5277..5819
                     /gene="ycbU"
                     /locus_tag="ACHK07_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101906.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANIVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5827..6342
                     /gene="ycbV"
                     /locus_tag="ACHK07_001561"
     CDS             5827..6342
                     /gene="ycbV"
                     /locus_tag="ACHK07_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8101907.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6308..7045
                     /gene="ycbF"
                     /locus_tag="ACHK07_001562"
     CDS             6308..7045
                     /gene="ycbF"
                     /locus_tag="ACHK07_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8101908.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSLRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7156..8166
                     /gene="pyrD"
                     /locus_tag="ACHK07_001563"
     CDS             7156..8166
                     /gene="pyrD"
                     /locus_tag="ACHK07_001563"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO8101909.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8340..8882
                     /gene="zapC"
                     /locus_tag="ACHK07_001564"
     CDS             8340..8882
                     /gene="zapC"
                     /locus_tag="ACHK07_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO8101910.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8879..9988)
                     /gene="ycbX"
                     /locus_tag="ACHK07_001565"
     CDS             complement(8879..9988)
                     /gene="ycbX"
                     /locus_tag="ACHK07_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO8101911.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10232..12340
                     /gene="rlmKL"
                     /locus_tag="ACHK07_001566"
     CDS             10232..12340
                     /gene="rlmKL"
                     /locus_tag="ACHK07_001566"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO8101912.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12352..14259
                     /gene="uup"
                     /locus_tag="ACHK07_001567"
     CDS             12352..14259
                     /gene="uup"
                     /locus_tag="ACHK07_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8101913.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14389..15642
                     /gene="pqiA"
                     /locus_tag="ACHK07_001568"
     CDS             14389..15642
                     /gene="pqiA"
                     /locus_tag="ACHK07_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO8101914.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15647..17287
                     /gene="pqiB"
                     /locus_tag="ACHK07_001569"
     CDS             15647..17287
                     /gene="pqiB"
                     /locus_tag="ACHK07_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO8101915.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17284..17847
                     /gene="pqiC"
                     /locus_tag="ACHK07_001570"
     CDS             17284..17847
                     /gene="pqiC"
                     /locus_tag="ACHK07_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO8101916.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            18103..18270
                     /gene="rmf"
                     /locus_tag="ACHK07_001571"
     CDS             18103..18270
                     /gene="rmf"
                     /locus_tag="ACHK07_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO8101917.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18340..18858)
                     /gene="fabA"
                     /locus_tag="ACHK07_001572"
     CDS             complement(18340..18858)
                     /gene="fabA"
                     /locus_tag="ACHK07_001572"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO8101918.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFNKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18927..20687)
                     /gene="ycbZ"
                     /locus_tag="ACHK07_001573"
     CDS             complement(18927..20687)
                     /gene="ycbZ"
                     /locus_tag="ACHK07_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8101919.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20873..21325
                     /gene="matP"
                     /locus_tag="ACHK07_001574"
     CDS             20873..21325
                     /gene="matP"
                     /locus_tag="ACHK07_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO8101920.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21401..22456)
                     /gene="ompA"
                     /locus_tag="ACHK07_001575"
     CDS             complement(21401..22456)
                     /gene="ompA"
                     /locus_tag="ACHK07_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO8101921.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKAHNNVTGESEKNHDTGVSPVFAGGVEWAITPE
                     IATRLEYQWTNNIGDAHTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK
                     HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQ
                     GLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRR
                     VEIEVKGIKDVVTQPQA"
     gene            complement(22813..23322)
                     /gene="sulA"
                     /locus_tag="ACHK07_001576"
     CDS             complement(22813..23322)
                     /gene="sulA"
                     /locus_tag="ACHK07_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO8101922.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23541..24170
                     /gene="sxy"
                     /locus_tag="ACHK07_001577"
     CDS             23541..24170
                     /gene="sxy"
                     /locus_tag="ACHK07_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO8101923.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(24133..26295)
                     /gene="yccS"
                     /locus_tag="ACHK07_001578"
     CDS             complement(24133..26295)
                     /gene="yccS"
                     /locus_tag="ACHK07_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO8101924.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26305..26751)
                     /gene="yccF"
                     /locus_tag="ACHK07_001579"
     CDS             complement(26305..26751)
                     /gene="yccF"
                     /locus_tag="ACHK07_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO8101925.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26874..28928
                     /gene="helD"
                     /locus_tag="ACHK07_001580"
     CDS             26874..28928
                     /gene="helD"
                     /locus_tag="ACHK07_001580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO8101926.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28960..29418)
                     /gene="mgsA"
                     /locus_tag="ACHK07_001581"
     CDS             complement(28960..29418)
                     /gene="mgsA"
                     /locus_tag="ACHK07_001581"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO8101927.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29514..30176)
                     /gene="yccT"
                     /locus_tag="ACHK07_001582"
     CDS             complement(29514..30176)
                     /gene="yccT"
                     /locus_tag="ACHK07_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO8101928.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30349..30762
                     /gene="yccU"
                     /locus_tag="ACHK07_001583"
     CDS             30349..30762
                     /gene="yccU"
                     /locus_tag="ACHK07_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO8101929.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30807..31124)
                     /gene="hspQ"
                     /locus_tag="ACHK07_001584"
     CDS             complement(30807..31124)
                     /gene="hspQ"
                     /locus_tag="ACHK07_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO8101930.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31182..32372)
                     /gene="rlmI"
                     /locus_tag="ACHK07_001585"
     CDS             complement(31182..32372)
                     /gene="rlmI"
                     /locus_tag="ACHK07_001585"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO8101931.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32467..32745
                     /gene="yccX"
                     /locus_tag="ACHK07_001586"
     CDS             32467..32745
                     /gene="yccX"
                     /locus_tag="ACHK07_001586"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO8101932.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32742..33071)
                     /gene="tusE"
                     /locus_tag="ACHK07_001587"
     CDS             complement(32742..33071)
                     /gene="tusE"
                     /locus_tag="ACHK07_001587"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO8101933.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33162..33821)
                     /gene="yccA"
                     /locus_tag="ACHK07_001588"
     CDS             complement(33162..33821)
                     /gene="yccA"
                     /locus_tag="ACHK07_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO8101934.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34229..35248)
                     /locus_tag="ACHK07_001589"
     CDS             complement(34229..35248)
                     /locus_tag="ACHK07_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8101935.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35217..35468)
                     /locus_tag="ACHK07_001590"
     CDS             complement(35217..35468)
                     /locus_tag="ACHK07_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO8101936.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            complement(35535..37973)
                     /locus_tag="ACHK07_001591"
     CDS             complement(35535..37973)
                     /locus_tag="ACHK07_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8101937.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AKCKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQQETEQNG
                     QKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCGAVMGDATYQET
                     FNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREITS
                     TSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDIS
                     TIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILR
                     RSYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFEGERHNALHDARYQAKYV
                     SAIWQKLFPNQADF"
     gene            complement(38066..38254)
                     /locus_tag="ACHK07_001592"
     CDS             complement(38066..38254)
                     /locus_tag="ACHK07_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8101938.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(38251..38439)
                     /gene="dicB"
                     /locus_tag="ACHK07_001593"
     CDS             complement(38251..38439)
                     /gene="dicB"
                     /locus_tag="ACHK07_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8101939.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(38450..39304)
                     /locus_tag="ACHK07_001594"
     CDS             complement(38450..39304)
                     /locus_tag="ACHK07_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8101940.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            complement(39823..39975)
                     /locus_tag="ACHK07_001595"
     CDS             complement(39823..39975)
                     /locus_tag="ACHK07_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8101941.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            40250..40552
                     /locus_tag="ACHK07_001596"
     CDS             40250..40552
                     /locus_tag="ACHK07_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO8101942.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            40555..40914
                     /locus_tag="ACHK07_001597"
     CDS             40555..40914
                     /locus_tag="ACHK07_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8101943.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(40961..41353)
                     /locus_tag="ACHK07_001598"
     CDS             complement(40961..41353)
                     /locus_tag="ACHK07_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8101944.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            41480..41740
                     /locus_tag="ACHK07_001599"
     CDS             41480..41740
                     /locus_tag="ACHK07_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8101945.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANEQAAAGVDS"
     gene            41737..42174
                     /locus_tag="ACHK07_001600"
     CDS             41737..42174
                     /locus_tag="ACHK07_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8101946.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            42261..43271
                     /locus_tag="ACHK07_001601"
     CDS             42261..43271
                     /locus_tag="ACHK07_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO8101947.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAERKVFTQVQWEQKFARHVQHVRAQVKPVSKGGSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            43264..43725
                     /locus_tag="ACHK07_001602"
     CDS             43264..43725
                     /locus_tag="ACHK07_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8101948.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKYPKGPTPIEWLKQEYERRKAAGFI"
     gene            43759..44520
                     /locus_tag="ACHK07_001603"
     CDS             43759..44520
                     /locus_tag="ACHK07_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8101949.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            44553..44834
                     /locus_tag="ACHK07_001604"
     CDS             44553..44834
                     /locus_tag="ACHK07_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8101950.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            44831..45136
                     /locus_tag="ACHK07_001605"
     CDS             44831..45136
                     /locus_tag="ACHK07_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO8101951.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            45123..45773
                     /locus_tag="ACHK07_001606"
     CDS             45123..45773
                     /locus_tag="ACHK07_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8101952.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGVQS"
     gene            45770..46099
                     /locus_tag="ACHK07_001607"
     CDS             45770..46099
                     /locus_tag="ACHK07_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101953.1"
                     /translation="MRASISYVDDCHLSVRVDEIVSSVPTFPTKNAAVNAGAPFGWRT
                     AVRIERRFENVWVVGKKCFQSDRSAGLNFEAYRFPFLRWEKEGGITKCPILSVRRFKQ
                     EATSEQD"
     gene            46086..46430
                     /locus_tag="ACHK07_001608"
     CDS             46086..46430
                     /locus_tag="ACHK07_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO8101954.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVGIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            46432..46785
                     /locus_tag="ACHK07_001609"
     CDS             46432..46785
                     /locus_tag="ACHK07_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101955.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            46782..47144
                     /locus_tag="ACHK07_001610"
     CDS             46782..47144
                     /locus_tag="ACHK07_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO8101956.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPDTKTAVLVAREFDRKGDWRMKWATYIPGHPDANDGWVVPGAS
                     WIPSHWMPLPEPPKEVRQ"
     gene            complement(47347..47646)
                     /locus_tag="ACHK07_001611"
     CDS             complement(47347..47646)
                     /locus_tag="ACHK07_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8101957.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(47652..47909)
                     /locus_tag="ACHK07_001612"
     CDS             complement(47652..47909)
                     /locus_tag="ACHK07_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8101958.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            48045..48323
                     /locus_tag="ACHK07_001613"
     CDS             48045..48323
                     /locus_tag="ACHK07_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101959.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            48325..49383
                     /locus_tag="ACHK07_001614"
     CDS             48325..49383
                     /locus_tag="ACHK07_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8101960.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            49384..49764
                     /locus_tag="ACHK07_001615"
     CDS             49384..49764
                     /locus_tag="ACHK07_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8101961.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            49761..50582
                     /locus_tag="ACHK07_001616"
     CDS             49761..50582
                     /locus_tag="ACHK07_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8101962.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            50809..51006
                     /locus_tag="ACHK07_001617"
     CDS             50809..51006
                     /locus_tag="ACHK07_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8101963.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNMYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
BASE COUNT        13200 a        11926 c        13299 g        12637 t
ORIGIN      
        1 atctttttct gaaaataaac taaagaaagg gctttatata tcacgcatat ttatttattg
       61 gatagtcatt agatgttgaa tcaatgaatt actcgttcag gtaatattta aatattcatg
      121 aaatctataa attaaagatt tgtcacttat tggatttagt atgcttgatt taaatggcgg
      181 acaattattg ttattattaa gtctaatgtc atttaaaggc attatcatat ttacgaagag
      241 atcaggatga taacgatgaa aaaaagtgta ttgacggcgt ttataactgt ggtatgtgca
      301 acgtccagtg ttatggcagc tgatgataat gctatcacgg atggttcagt aacatttaat
      361 ggtaaagtta ttgctccagc ttgtaccctg gtcgctgcga cgaaagattc cgtggtgact
      421 ttgccagatg ttagtgccac gaagttacaa accaatggtc aggtttctgg cgtgcaaact
      481 gatgtgccaa ttgaattaaa agattgtgat actaccgtaa caaaaaatgc aacgttcacc
      541 tttaatggca ctgcggatac tactcagatt acagcgtttg ctaaccaggc ctcatctgat
      601 gctgctacaa acgtggccct gcaaatgtat atgaatgatg gtacaacggc catcacgcca
      661 gacacagaaa ccgggaatat tttgttgcaa gatggagatc agacgttgac ttttaaagtt
      721 gattatatcg ctacggggaa agcgacttct ggtaatgtga atgcggtaac aaatttccat
      781 attaactatt attaatagaa ctcattaatt gttttattaa ttagtacccc tccagtgttc
      841 tggaggggat attcatattt tttaagagtg actatttatg aaaacttgca taacaaaggg
      901 aattgtgacc gtaagtttaa cggcaatatt actcagctgc tcatccgcat gggcggcagg
      961 taaaggcggg attggacttg cagcaacacg tcttgtttat tcagaaggtg aagagcaaat
     1021 ttcactgggt gtacgtaaca ccagtccgga tgttccttat cttattcagt catgggtgat
     1081 gaccccagat aataaaaaat cagcagactt tattattaca ccaccgctat ttgtgctgaa
     1141 tccggctaat gagaatctgt tacgcattat gtacattggt gcgccgttgg cgaaagacag
     1201 agaaactctt ttcttcacta gcgtacgggc agtcccttca acaacgaagc gggaagaagg
     1261 caataccctg aagattgcca cacaaagcgt catcaaactt ttctggcgac caaaaggttt
     1321 agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc acttcgtcag ctgacatggt
     1381 tacggtcagt aacccaacac cttatttcat taccctgaca gacctgaaaa taggtggaaa
     1441 ggtagttaaa aatcaaatga tttccccctt tgataaatac caattctctc tgccaaaggg
     1501 tgccaaaaat agcagcgtaa cgtatcgaac catcaatgac tacggggcgg aaacgccgca
     1561 actcaactgt aaatcgtaat tcgtcttcag ttaagagagc gagatgtata gaactcaccg
     1621 acaacacagc ctgttaagct ctggtggcgt gccatcgttt attggtgggc tggtggtgtt
     1681 tgtgtcggca gcgttcaatg cacaagctga aacctggttc gatccagcct ttttcaaaga
     1741 tgatccctca atggtggccg atttgtctcg tttcgaaaaa ggacaaaaaa taacgccagg
     1801 ggtttatcgt gtcgatattg ttctgaatca gacaattgta gatacgcgca acgtcaattt
     1861 tgttgagata acgccagaga aggggattgc cgcctgtttg acgaccgaaa gcctggatgc
     1921 aatgggcgtg aatactgatg catttccggc ttttaaacaa ctggacaaac aagcgtgtgc
     1981 gctattggcg gagattattc cggatgccag cgtaactttt aatgtgaata aactccgtct
     2041 ggaaatttca gtaccgcaaa ttgccatcaa aagtaatgct cgtggttatg tcccccctga
     2101 acgttgggat gaagggatca acgcgctatt actgggatat tcatttagcg gggctaacag
     2161 tattcatagc agcgcagaca gtgattctgg cgacagttat tttctgaatt taaacagtgg
     2221 cgttaattta ggcccatgga gattgcgcaa caattcaaca tggagtcgta gtagtggcca
     2281 aaccgcagaa tggaagaatc tcagcagcta tttgcaacgg gcggttattc cactaaaagg
     2341 cgaactgacc gtaggtgatg attatactgc aggcgatttt ttcgatagcg ccagctttcg
     2401 tggtgtgcag ctggcatcag atgacaacat gctgccagac agcctgaaag ggtttgcgcc
     2461 tgtagtgcgt ggtatcgcca aaagcaatgc ccagataacg attaagcaaa atggttacac
     2521 catttaccaa acttatgtat cgcctggtgc ttttgaaatt agtgatctct attccacgtc
     2581 gtcgagcggt gatttgttgg ttgaaatcaa agaagcggac ggcagcgtca atagctatag
     2641 cgtgcccttt tccagcgtgc cattactcca gcgtcagggg cgaatcaaat acgcggtgac
     2701 actggcgaaa tacaggacca atagtaatga acagcaggag agcaaatttg cccaggtcac
     2761 gttgcagtgg ggcggaccgt ggggaacgac ctggtatggt ggtggacaat atgctgaata
     2821 ttaccgtgct gccatgtttg gtctgggatt taacctcggc gatttcggag caatttcgtt
     2881 cgatgcgacc caggcgaaga gtacgctggc agaccaaagc gaacataaag gtcagtcata
     2941 tcgttttctg tatgccaaaa cgctcaacca attgggcact aattttcaat tgatgggcta
     3001 tcgctattcg acgtcgggtt tctacaccct ttccgacacc atgtataaac atatggatgg
     3061 ctacgaattt aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt attacaattt
     3121 gttttacacc aaacgtggca aactgcaggt caatatctcc cagcaattag gcgagtacgg
     3181 ttcgttttat ttaagtggta gccagcaaac ttactggcat accgatcaac aggatcggct
     3241 attacagttt ggctataaca cgcaaattaa agatctctcg ctggggatct cctggaacta
     3301 cagtaagtcc cgtggtcaac ctgatgctga tcaggtgttt gcactaaatt tttccctgcc
     3361 gctcaatctg ttgctcccca gaagtaatga tagctatacc aggaaaaaaa attacgcctg
     3421 gatgacctct aacaccagta tcgataacga agggcacatt acacaaaacc tgggtttaac
     3481 ggagacacta ctcgatgatg gtaacctgag ctacagcgtg caacagggat ataacagcga
     3541 ggggaaaacg gctaatggta gcgccagcat agactacaaa ggggcgtttg cagatgcccg
     3601 agtgggctac aactacagcg ataacggcag tcaacaacaa ctgaactacg ctctttcagg
     3661 cagtttagtt gcccattcac agggcattac cctggggcaa tcgctggggg aaactaacgt
     3721 gctgattgca gcaccaggcg cagagaatac tcgtgtggcg aacagcaccg ggctgaaaac
     3781 tgactggcgc ggatataccg ttgttcctta tgccacttct tatcgtgaaa attgaatcgc
     3841 acttgatgcg gcgtcgttaa aacgtaacgt ggatcttgaa aatgcagtag tcaacgtggt
     3901 tcccaccaaa ggggcgttgg ttctggcgga gttcaatgcc catgcgggag caagggtatt
     3961 aatgaaaaca acaaagcagg gtataccgct gcgttttggc gcgatagcga cgctggacgg
     4021 ggtacaggct aatagcggca taattgatga tgatggctcg ctctatatgg cgggtttacc
     4081 ggcgaaggga acaataaccg tacgctgggg cgaagcaccc gatcaaattt gtcatatcag
     4141 ttacgagctt accgaacaac aaattaactc tgcgattacg cgaatggatg ccatatgcag
     4201 ataatctttg gagaaaaatg cgtgtcatta ctacgactat tttttgccgc cgtcttaatg
     4261 ctatggtgcg ctcaaaccgc tgcttatagc gggcagtgtc ataccactca ggggaatccg
     4321 tatattggcg tcaattttgg cgttaaaacc ctggaggaag aagaaaatac tgcaggggta
     4381 gttaaagaca aattttatca gtggaacgaa tcgaatgatt attatgtttc ctgtgattgc
     4441 gataaagaca atgtcagaaa tggtcgatgg gcattcgccg cggattcacc gttagtctat
     4501 ttaggcgaca actggtacaa aattaatgac tatcttgccg ccaaagtttt attgcaggtt
     4561 aaaggcagtt ctcctactgc ggttcctttc gaaaacgtgg gcacaggggg ggatacccga
     4621 tggcatattt gcgaccctgg cggtcaacgt ttaggcggcc agggggctag cggtaatagc
     4681 ggtagctttt ccctgaaaat attgcagccg ttcgttggct cggtcgtcat tcctcctatg
     4741 gcgctggcgc gattatttga atgctacaac atacctgcag gtgattcctg cacgactaca
     4801 ggtacaccgg ttttagtgta ttacctgtct ggtacgatca attcacttgg ctcatgttcc
     4861 gtcaatgccg gagaaacaat cgaggttgat ctgggcgacg tatttgcggc taactttcgt
     4921 gttgtagggc ataagcctct tggggccaga acggcagaac ttgcaattcc ggtcaggtgt
     4981 aacacgggaa acgcggggtt agttaacgtc aacctgagtc tgactgcaac ctcagacccc
     5041 agctatcccc aggcgattaa gacgtcacgt ccaggcgtgg gcgtggtggt gaccgatagc
     5101 cagaacaaca ttatttcccc ggctggtgga acactaccgc tctctattcc tgatgatgca
     5161 gacagtatcg cgcgaataaa tgtctatcca gtcagcacga caggtgtacc accagaaacc
     5221 gggcgatttg aagccacggc aacggtgaga ataaattttg attaacgcgt gaacgtatga
     5281 agaaaaaaac gatatttcag tgcgttatct tgttctttag cattcttaac atccatgtcg
     5341 ggatggctgg gcctgaacaa gttagtatgc atatttatgg gaatgtggtc gatcagggct
     5401 gtgatgtcgc caccaaaagt gcattacaaa atattcatat tggtgatttt aatatcagtg
     5461 attttcaggc cgcgaatatc gtaagcactg ctgctgattt gaatatcgat atcaccggtt
     5521 gtgccgctgg tattactggc gcggacgtcc tttttagcgg cgaggctgac acccttgcgc
     5581 cgacactgct caaactaact gacacaggcg gaagcggtgg tatggcaacg gggattgccg
     5641 tgcaaattct tgatgcgcaa agtcagcaag aaatcccgct caatcaggtc cagcctctta
     5701 cgcccttaaa agccggggat aacacactca aatatcaact tcgttataag tccacaaagg
     5761 cgggagcaac gggcggtaat gcgacggcgg ttctctattt tgatctggtt taccagtgaa
     5821 ggagtgatgt tgaaacggat aatctggatt ctgttcttac tgggattaac ggggggttgt
     5881 gagctatttg cccatgatgg cacggtcaac attagcggat cgtttcgccg taatacatgc
     5941 gtgctggcac aggatagcaa gcaaatcaat gtgcagttag gcgatgtctc actgacacgt
     6001 ttttctcatg ggaattatgg cccggagaaa tctttcatca ttaatttgca ggattgcgga
     6061 acagatgtca gcacggttga cgtcaccttt tcaggaaccc ccgacggagt gcagagcgaa
     6121 atgttgtcca ttgaaagtgg tacggatgca gccagcgggc tggcaattgc gattctggat
     6181 gatgcgaaga tattgattcc gcttaaccag gcgagtaaag actacagcct acatagcggt
     6241 aaggtgccat taacttttta tgcgcaattg cgacctgtca atagcgatgt gcagtcgggt
     6301 aaagtgaatg ccagcgcgac atttgtactt cattatgact aatacgtgga acagattggc
     6361 gctcttgatt ttcgccgttt tatcgctgct ggtggcgggt gaattgcagg caggagtggt
     6421 ggtcggcgga acgcgattta tctttccggc agacagagaa tcgatatcta ttttactgac
     6481 taataccagt caggaatcct ggcttattaa cagtaaaatc aaccgcccaa cgcgttgggc
     6541 cgggggtgaa gcgtcgacag tgccggcacc attactggcc gctccgccac ttattctcct
     6601 gaagcccggt acgacaggca cgttgcgctt gctgagaacg gaaagcgaca tcttgcctgt
     6661 ggatcgcgaa acgctatttg agttaagcat tgccagcgtg ccatccggca aagttgaaaa
     6721 tcagagcgta aaagtggcga tgcgctcggt atttaaactg ttctggcgac ccgaaggttt
     6781 gccgggcgac ccgctggaag cttatcaaca attacgctgg acacggaatt cgcagggtgt
     6841 acaactcact aacccaacgc cttactacat taacctgatt caggtaagcg taaatggtaa
     6901 agcgttaagt aatgtgggag ttgtgccgcc taaaagcctg cgtcagacaa gctggtgtca
     6961 ggcaatagca ccgtgtcatg ttgcctggcg ggcgataaat gattacggcg ggttgagtgc
     7021 aaagaaggag caaaatctgc cctgaaacag gttcggaaaa cgtttgcgtt ttttttgccg
     7081 caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt gcacaccggg
     7141 aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca gctcgatcca
     7201 gagcgcgctc atgagtttac ttttcagcaa ttacgccgca ttaccggaac accgtttgaa
     7261 gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct gacgtttaaa
     7321 aatccgcttg gtctggcagc cggtcttgat aaagacgggg agtgcattga cgcgttaggc
     7381 gcgatgggat ttggatcgat cgagatcggt accgtcacgc cacgtccaca gccaggtaat
     7441 gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg tatgggcttt
     7501 aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aagcccatta tgacggcgtc
     7561 ctgggtatta acatcggcaa aaataaagat acgccagtag agcagggcaa agatgactat
     7621 ctgatttgta tggaaaaaat ctatgcctat gcgggatata tcgccatcaa tatttcatcg
     7681 ccaaataccc caggattacg cacactgcaa tatggtgaag cgctggatga tctcttaact
     7741 gcgattaaaa ataaacaaaa tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca
     7801 gtgaagatcg cgccggatct ttctgaagaa gaattgatcc aggttgccga tagtttagtt
     7861 cgccataata ttgatggcgt gattgccacc aataccacac tcgatcgttc tctggttcag
     7921 ggaatgaaaa attgcgatca aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc
     7981 accgaaatta ttcgccgctt gtcactggaa ttaaacggtc gcttaccgat catcggtgtt
     8041 ggcggcatcg actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg
     8101 caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat cgttacccat
     8161 atctaattat ttcttcgaat tcctctttat aagataatca gggctttatt ttcagccctg
     8221 gttgttttat attcatcact gttgcttatt tagacatttt gtacttttat tgttgaggtt
     8281 attaagcgaa gcgacaatgg attgtgttgc tgcggtttta taaggttggg gagaggcaga
     8341 tgcgaattaa accagacgat aactggcgtt ggtattacga tgaagagcac gatcgaatga
     8401 tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaaa atgttgactc
     8461 cagatgcctt ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat ttctcttttg
     8521 aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa agccgaactg gtgctgaatg
     8581 cactggtagc aattcgctat ctcaaaccgc aaatgccgaa aagctggcat tttgtttccc
     8641 atggtgaaat gtgggtgccc atgccggggg atgcggcctg cgtttggttg agcgacacta
     8701 acgagcaagt gaatttactg gtcgttgaat ctggcgagaa tgccgcgcta tgcctgctgg
     8761 cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca
     8821 tgaacgacag gctgaaaccg caggttaatg ttgacagctt cagcctcgaa caggcagtct
     8881 aacgcgccaa cttaagtgca gtcttcggta cacagctaca gcaaagaatg gtgccatcat
     8941 cgcccattgc tgattttttc agcggcgtga cttcgccttc taaaagctga acacggcaac
     9001 ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt tctaattgct
     9061 ccagcaacac ctgttggtta tttccacgaa atgcctgtcc ctgccagtca atatctacat
     9121 tggcgtccgg ttgttgcgtg atgttggcag tatcatcagc ggcagctgcg ccgtaaattt
     9181 ttgccggagc cgttgtcaga atttccacct catcgccaac gcgaatcacg ccgctattac
     9241 gggcaattaa attctgacca aaatcgacat cgccgttatc ctgggcagtg cggaaagatt
     9301 gcaatgtttt taatggttcg cctgccggat gtttttgccc tttttctggg ctgacggtgg
     9361 tgaaaataca gcggctacaa ggtttaacca catcaaacac cacatcacca atgcgaatca
     9421 ctttccaact atcttcttcc caggctgacg cgccggaaac caccagattg gggcggaatt
     9481 gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag gcttcgttag
     9541 caagaaggta aggatagcca tcagcaaatg acaggggtac agtgttgtgg cgtttcacgc
     9601 gccgggtcat ttgtggcccc acccagcgta attgcacttc gcgggagaaa aatccactta
     9661 accatttgtt gatcgcttct ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg
     9721 ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg ccatctggtg
     9781 cggtgagatg caagccatca tgcacaggtg aaggagtaaa ccgtaccatc tggggaaact
     9841 ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca
     9901 gaccactgac atcagccaga gcatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat
     9961 gaataaaaag ccgggttaat gtcgccacgg tgtggtcctc aaatgaaaat aagccctcaa
    10021 ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag cgcctgtccc
    10081 attaggctat tttatttgtc agtttggccc tgggttgtgc tcgaaatcct cacgtacttg
    10141 tgtacgctcc ggtttctccg cgctgtccat gtccagactg actgcaacaa ttacgcctac
    10201 tgcggtaggc tcttagagta aaagtgacga tatgaattct ctgtttgcca gtacggcccg
    10261 tgggctggaa gagctgttaa aaactgaact ggaaaacctg ggggccgttg aatgccaggt
    10321 ggttcagggt ggggtccatt tcaagggcga cacacggctt gtttaccaga gcctgatgtg
    10381 gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt acagcgattt
    10441 agacctctat ctcggtgttc aggcgatcaa ctggacagag atgtttaatc ctggcgcgac
    10501 cttcgctgtc cacttcagtg gtttgaatga cacaatccgc aacagtcagt acggcgcgat
    10561 gaaagtgaaa gacgcaatcg tcgatgcttt cacgcggaaa aatctgccgc gtccaaatgt
    10621 tgatcgcgat gcgccggata tccgcgttaa cgtctggctg cataaagaaa ccgccagtat
    10681 cgctcttgat ctgagtggtg atggtttaca tctgcgtggc tatcgcgatc gtgctggtat
    10741 tgcgccgatc aaagaaaccc tggcagccgc tattgtgatg cgatccggct ggcagccagg
    10801 aacaccgctg ctcgatccga tgtgtggttc cggtacgttg ctgattgaag cggcgatgct
    10861 ggcgaccgat cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg gctgggcgca
    10921 gcatgatgaa gctatctggc aggaagtgaa agcagaagcg caaactcgcg cccgtaaagg
    10981 cctggctgag tatagctctc atttttacgg ttcggacagc gacgcacggg tgattcaacg
    11041 tgcgcgcact aacgcccgtc ttgcggggat tggtgaactg atcacctttg aggtgaaaga
    11101 tgtcgcgcaa ctgaccaatc cgctgccgaa agggccgtac ggcacagtgt tgagcaaccc
    11161 gccatacggg gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata gcctgctggg
    11221 tcggatcatg aaaaaccagt ttggtggctg gaatctctct ttgtttagtg cctcgccgga
    11281 tctgctaagc tgcttgcagc tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct
    11341 ggactgcgta cagaaaaatt accacgttgc cgaaagcact ccagacagca aaccggcgat
    11401 ggtagcggaa gattacgcca accgcctgcg taagaacctc aaaaaattcg agaagtgggc
    11461 tcgccaggaa ggaattgaat gttaccgcct gtatgacgcc gatctgccgg aatataacgt
    11521 tgccgttgac cgttatgccg actgggtggt ggtgcaggag tatgcaccgc caaaaactat
    11581 tgatgcgcac aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca tttcggtact
    11641 ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa cgccagaagg gcaaaaacca
    11701 gtaccagaaa ctgggcgaga agggcgagtt tcttgaagtt accgaatata acgctcactt
    11761 gtgggtgaac ctgacggatt atctcgatac tggtctgttc ctcgatcacc gtatcgcccg
    11821 ccgtatgctc ggtcagatga gcaaaggcaa agatttcctc aacctgtttt cttataccgg
    11881 cagcgccacc gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg tggatatgtc
    11941 gcgtacttat ctggaatggg cagaacgcaa cctgcgtctg aatggcctga ccgggcgtgc
    12001 gcatcgcctg attcaggccg attgcctggc gtggctgcgt gaggcaaatg aacagttcga
    12061 tctgatcttt atcgatccgc caaccttctc taactcaaaa cgaatggaag atgcgtttga
    12121 tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg
    12181 gacgatcatg ttctcgaaca acaaacgtgg cttccgtatg gatctcgacg gcctggcaaa
    12241 actgggactg aaagcacaag aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa
    12301 ccgccagatc cacaactgct ggctgattac cgcagcctga aaggaatagt aatgtcatta
    12361 atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa
    12421 ctgcatatcg aagataacga acgtgtttgt ctggtgggcc gcaacggcgc aggcaaatcg
    12481 acgttaatga aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat tatttacgag
    12541 caggatttga ttgtagcgcg tctgcaacag gatccgccgc gtaacgttga gggtagtgtt
    12601 tatgatttcg ttgccgaagg cattgaagaa caagcggaat acctgaaacg ctatcacgat
    12661 atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact ggcaaaggtt
    12721 caggaacagc tggatcacca caacctgtgg cagctggaaa accgcatcaa cgaagtgctg
    12781 gcgcaactgg ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt
    12841 aaagcggcat taggacgcgc gctggtgagc aatccgcgcg tattgctgct ggacgaaccg
    12901 actaaccatc tggatatcga aaccatcgac tggctggagg ggttccttaa aaccttcaat
    12961 gggacgatta ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt
    13021 gttgatctcg atcgcggcaa gctggtgacc tatccgggca attacgatca gtacctgctg
    13081 gaaaaagaag aagccctgcg cgtggaagaa ctacaaaatg ccgagttcga tcgcaaactg
    13141 gcgcaggaag aagtgtggat tcgccagggg atcaaagcac gccgtacccg taatgaaggc
    13201 cgcgtacgcg ccctgaaagc gatgcgtcgc gagcgtggcg aacgtcgcga agtgatgggt
    13261 accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt cgaaatggaa
    13321 gacgtttgct accaggttga cggaaagcaa ctggtgaaag atttttctgc ccaggttcta
    13381 cgtggcgaca aaattgccct gattgggccg aatggctgcg gcaaaaccac gctgctgaaa
    13441 ctgatgctcg gtcagcttca agcggacagc gggcgtattc acgttggcac caaactggaa
    13501 gtggcttatt tcgatcagca ccgcgcggaa cttgatcccg ataaaacggt gatggataac
    13561 cttgccgaag gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt tttgggctat
    13621 ttgcaggact tcctgttcca tccgaaacgg gcgatgacgc cagtacgtgc gctttctggc
    13681 ggtgagcgga accgcttgct gctggcgcgt ttgttcctca aaccaagcaa cttattgatt
    13741 cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg agctgctgga agaactgatc
    13801 gacagctacc agggcacggt attgctggta agccacgatc gtcagtttgt tgataacacc
    13861 gttacagaat gctggatctt cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat
    13921 catgacgccc gtggtcagca agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa
    13981 aacgaagaag ccgccgcgcc aaaagcagaa actgtaaaac gcagcagtag caaactaagc
    14041 tataaattgc agcgcgaact ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag
    14101 ctggaagccc tacagacgca ggtggcggat gcttccttct tcagtcagcc gcatgagcag
    14161 acgcaaaaag tgctcgctga tatggctgcc gcagaacagg aactggagca agcctttgaa
    14221 cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa
    14281 cagagacagc tgacaacggt agaattgcct gatgcactgg gtttatcagg catgaaaacc
    14341 aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat gtgcgaacat
    14401 catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtagc gttaccgcgc
    14461 cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg
    14521 gatgcccccc ggcagcgtcc taccgcctat gcgttggctg cactgttcat gctgttgctg
    14581 tccaacttgt ttccttttgt gaatatgaac gttgcaggag ttaccagtga aattacatta
    14641 ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac ctttttcctg
    14701 ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact ggtgaatcgc
    14761 gcggaattac cggttcgttt aaaagagcaa ctggcacggg tgctttttca actcaaaacc
    14821 tggggaatgg cggagatttt cctcgcgggt gtgctggtca gtttcgttaa actgatggct
    14881 tacggcagca ttggcgtagg cagcagcttt ctcccctggt gtttattttg tgtcctgcaa
    14941 ctgcgtgctt ttcagtgcgt tgatcgtcgc tggttatggg acgatatcgc cccgatgcca
    15001 gaactgcgcc agccgctaaa accaggcgtc acggggatac gtcaggggct gcgttcgtgc
    15061 tcctgttgta cggcaatcct tcctgctgat gaacccgtgt gcccgcgctg tggtactaaa
    15121 gggtacgttc gacgtagaaa cagcctgcaa tggacactcg cgctgcttgt tacgtccatc
    15181 atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt aggctcgaag
    15241 atgccatcga cgattctcgc tggggtcatc ctgttatgga gcgaaggctc ttatcccgtc
    15301 gctgcggtga tcttccttgc cagtattatg gtgccaacgt taaagatgat cgccatcgcg
    15361 tggctgtgtt gggatgccaa agggcatggc aagcgcgaca gtgaaagaat gcatttgatt
    15421 tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt tatcgcggtg
    15481 ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat gggtgcatta
    15541 atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga cccgcgtttg
    15601 tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg aatctaataa
    15661 tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt
    15721 cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg gaccggaagt
    15781 gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg
    15841 tagcgttgac gtcggcgtgg ttgaaagcgc cacactcgct gatgatttga cacacgttga
    15901 aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagata ccgtcttttg
    15961 ggtggtgaaa ccgcagattg gtcgagaagg gattagcggt ctgggaaccc tgctgtctgg
    16021 ggtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat acgatttgct
    16081 ggactcgcca ccgttggccc cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa
    16141 aaaagccggg cagctctcgc caggagatcc ggtgctgttc cgtggctatc gggtaggttc
    16201 ggttgaaacc agcaccttcg atacgcaaaa acgcaatatc agttatcaac tgttcatcaa
    16261 tgcaccttat gaccgactgg tgaccagcaa tgttcgcttc tggaaagata gtggcattgc
    16321 ggttgatctg acgtcagcgg gaatgcgtgt ggagatgggc tcattgacaa cgctgctgag
    16381 tggcggtgtc agctttgatg tgccggaagg tctggattta gggcagccag tggcaccgaa
    16441 aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt acaccgatca
    16501 cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt
    16561 agaattccgg ggtattcgcc tcggtaccgt aagcaaagtg ccattctttg cgccgaatat
    16621 gcgtcagaca tttaacgatg attaccgtat tccggtgctg attcgtatcg agccagagcg
    16681 gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg aattgttgaa
    16741 acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggcgcac tgtatgttga
    16801 tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta atggttatca
    16861 gattatcccg accgttagcg gcggcctggc gcaaatccag caacgactga tggaagcgtt
    16921 ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca gtacgctttc
    16981 tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga acaagatcct
    17041 cgccagccag tcgatgcagc agttgccgac ggatatgcag tcaacgttgc gtgaattgaa
    17101 tcgcagcatg cagggcttcc agcccggctc cgcagcctac aacaagatgg tggcggatat
    17161 gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa
    17221 gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga agagggcgaa
    17281 acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg cagctccggc
    17341 gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca aagtaccgcc
    17401 agccagggca atcgcctgtt atgggtagag caggtcgctg tacctgacta tctggcgggg
    17461 aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg
    17521 gccagcccgt tggatcaaca gttgcgcaac accctggtag ccaacctgag cacgcaactg
    17581 cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggacacgct caatgttacc
    17641 gtaacggagt ttaacggtcg ctatgatggc aaggtcattg tcagtggtga gtggctgttg
    17701 aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggagtgca aactcaggat
    17761 ggttacgatg agatggttaa agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt
    17821 gcacaagaga taaagcgtct accttaatta taaagatttg taaatataac cgtctccggt
    17881 atgttgcctg aggcggtttt tttgtttcta acgtgcggaa aaatttgttc ctcttcacat
    17941 tttttgtaca accgacatgc ccgtgtagct cacaaatatg acagtggcgt gaattttgcg
    18001 cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt tagtaatcac
    18061 tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag acaaaaacga
    18121 gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa
    18181 gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa
    18241 gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa acctccgcat
    18301 tgcggaggtt tcgccttttg atactctgtc tgatgataat cagaaggcag acgtatcctg
    18361 gaacagaccg actttcaggt cgctggcggt atagatcaga cgaccatcaa ccagcacttc
    18421 gccatctgcc agacccataa tcagacgacg attaacaatg cgtttaaagt gaatacggta
    18481 ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc caacgcccag
    18541 cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca gctgccacat
    18601 tgcgtccagg cccaggcatc ccggcataac cggatcgcca ataaagtggc atccgaagaa
    18661 ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgttga agttaccacc
    18721 cgtttcggtc attttgacca cacggtccat catcagcatg ttcggtgctg gcaattgcgg
    18781 gcctttagcg ccaaacagtt caccgcgacc agaggcaaga aggtcttctt ttgtatagga
    18841 ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg caggatagct
    18901 aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag ccaacgtaat
    18961 ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg
    19021 gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa taacggcagt
    19081 gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac cgctttcacc
    19141 agttcactgt gaagacttaa atggcgaacg ttagcagtgg ggataatgac accttgttta
    19201 cccgttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc atttaaacca
    19261 ccgaccggct gggcgcgacc gaactgatcg actgaacctg tgatagcgat actctgattc
    19321 accggcacat cggcgagggc gcttatcaga gcgcagagtt cagccatcga ggcactatcg
    19381 ccatccactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc
    19441 tcaagctgta gttccgacat caggaacgcc tgcatgatca tcatcccttt cgcatggata
    19501 ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca
    19561 acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc
    19621 gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat ttgctcctga
    19681 aggatctcat cctgcatgcg ttcagcgagg aaaccttcgc gccattcacg ctgctgtagc
    19741 attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc gacttcttta
    19801 cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt
    19861 gctgcttcgc ggataagtat cggccaggca tccgctcccg gtgcaggtaa gtgattatgt
    19921 ctggcggtaa atgtcaccca gcgacaccac tggcttactg actccgcatc gacaatctgg
    19981 agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc catctcctgg
    20041 aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa
    20101 ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg
    20161 ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt acgcaaagag
    20221 atgatgagaa taccgccatt tgcctgatgc accagaccag gctgcagggt aatgtcgcca
    20281 ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc agcgacaact
    20341 tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt tacgtaagcg gatggtgtgg
    20401 ccggaaacct catagtgacc gcccaccagt tggcctgcat cactttgtag cgcacgtgcc
    20461 gcatcggcaa taagattcag atactcagac tcttccgggg ccttcgccag cataaaagag
    20521 gaggatgctc gcgtatgcag caactgctcc agcgcaaatt gcagtcgcgg ttgagtatca
    20581 ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc ctgatagcta
    20641 tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt tgatgttttt
    20701 tagtctgttg tcaaagccgc gattataccg taaccggcac tacagcacac gtagaaagca
    20761 ccgacaatac tcctggcatg ggcgttaaag ctcacaggat ggagattctt ttcttcactg
    20821 gcctaaaaag ctgatattct gtaaagagtt acacggtaac attgagatcg ctatgaaata
    20881 tcaacaactt gaaaatcttg aaagcggttg gaaatggaag tatctggtga agaagcatcg
    20941 cgaaggggag ttaattaccc gttacataga agccagtgcc gcccaggaag ccgtcgatgt
    21001 gttgctctcg ctggaaaatg aacccgtgct ggtaaatggc tggattgaca agcatatgaa
    21061 tccggaactg gtcaatcgca tgaagcaaac cattcgggcc agacggaagc gccattttaa
    21121 tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg gaatttattg tctggcaacg
    21181 tctggctggt cttgcgcagc gtcgcggtaa aacactttct gaaacgattg ttcagctgat
    21241 tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg tcttctttga agcaggatct
    21301 gcaggcattg ctgggtaagg aataactgat gaaactcagt tcaatttact aaaggcgaaa
    21361 aaaaaccccg cagctgcggg gttttttacc agacgagaac ttaagcctgc ggctgagtta
    21421 caacgtcttt gataccttta acttcgatct caacgcgacg atccggagcc aggcagtcga
    21481 tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc agtaaccggg ttggattcgc
    21541 ccataccacg tgcggagatc ttgtctgccg ggataccttt ggagatcagg taatcaacaa
    21601 cagactgagc acggcgctcg gacagaccct ggttgtaagc gtcagaaccg atgcggtcgg
    21661 tgtaacccag aacaactacg gaaccgtctt tcggatccag gttgctcagc tggctgtaca
    21721 gctgatccag agcagcctga ccttccggtt tcagggttgc tttgttgaag ttgaacagaa
    21781 cgtcagactt cagagtgaag tgcttggtct gtacttccgg tgccggagct ggagccggag
    21841 caactaccgg agctgcttcg ccctgaccga aacggtagga aacacccagg ctcagcaggc
    21901 cgttgtccgg acgagtgccg atggtgtgtg cgtcaccgat gttgttggtc cactggtatt
    21961 ccagacgggt agcgatttca ggagtgatgg cccattcaac accacctgcg aataccggag
    22021 aaacgccggt atcgtggttt ttctcagatt cacctgtcac attgttgtga gctttggtgt
    22081 ctgcacgcca aaccatacca cccagacgag tgtacacgtc caggtcgtca gtgattgggt
    22141 aacccagttt agcggtcaac tgaacgccct gagctttgta tgcaccgttt tcaacgctgc
    22201 ctttgtacgg catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa
    22261 cctggtaacc accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt
    22321 cgatgaaacc agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt
    22381 tatctttcgg agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga
    22441 tagctgtctt tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc
    22501 aacgagataa cacggttaaa tccttcaccg ggggatctgc tcaatattaa ctctaccgat
    22561 atcttcggct tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt
    22621 acaagtgtga actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct
    22681 tttttgtcag cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc
    22741 aaggaaaatt aatcacgact gaaagcattg gctggacgac aaaaaaagtt ccaggattaa
    22801 tcctaaattt acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt
    22861 tgcgtgagag gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc
    22921 cgcatcaaca agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac
    22981 cacactgtaa ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaaggggg
    23041 gagctggcta atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg
    23101 actcagtttt tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg
    23161 taacaatggc aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc
    23221 actgataagc ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga
    23281 tgagaacgac gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt
    23341 gagttactgt atggatgtac agtacatcca gtgacgacaa agatcaaccc tattttcgga
    23401 aagagcctcg caaattttgt cgttggtgac gggaaaacat aaattaatct tgcccctaaa
    23461 gaataagttg cctattttcg tatttaacgg atccgttaat gtgaaccatt cttttatgtt
    23521 atgattttaa aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag
    23581 aatacctggc aacgttgggc acaattgaat accgatcatt gtttggtagt tacagcctga
    23641 ccgttgacga cacggtgttt gcgatggttt ctgatggtga gttatatctt cgggcttgtg
    23701 agcaaagtgc acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg
    23761 gccgatccgt taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga
    23821 agctggtgcg tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca
    23881 atacccggga aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg
    23941 gggaggtggg gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt
    24001 tgcgactgcg gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg
    24061 ccattatcgg cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat
    24121 gggctgactc tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg
    24181 cagacgacca atctcaggca gtaatgcaat caataaccca acttgttgta cgaccagagg
    24241 ttctttgctg tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc cagctaatgc
    24301 ctgattgacg cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac
    24361 tgcgtcatca agaaatgcca gaatttcagg attagttaac tgctcccggt gagcaccgag
    24421 ggctgagata tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg
    24481 aatttgcggg gtaacgttcg gttcgctgga catatttgat accaccgacg ccagttcagc
    24541 atcacggttg tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg
    24601 atggtattgc tccagtatgg catcgagata gcgacagttg gcctctgtgg cgcgttcgag
    24661 catgcgcggc agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc
    24721 aatggcacaa ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc
    24781 cagtaagtta aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gagcgtattg
    24841 cacgttacgg aaggcaaaaa agagcacgcc ggtaataacc agcagcacca gctgcccttc
    24901 cagtgatggc acaaaccaca gcacaggaat gccaatggcg atacctacca gcgtaccaat
    24961 aatccttaac ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact
    25021 tgtcagcaag atccaatacc cgtgatgcat tccggttatt tgaatgatgg cgtagccgaa
    25081 gcacaacacc agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg
    25141 acggctaaga cgcagccaga tatcactcaa tccgtgcggg ctgtcatcag cgagctcatt
    25201 ttcgtcatta ttatggggca gtgcctgggc ctgttctgat tcaattgttg ccagttgggc
    25261 atcaatggcg cgtaaattgt tcagcaaaaa tcccagtgtt ttgagtaaat cggcaggcgc
    25321 gccgttatcg cgcatccgct ccagggcagc atcaatatgc gtaaaagcgc gctcaaaatg
    25381 cggatcatgt tgataaggct gacgcagcaa aatacagcgt gacagttgct ggcacgcctg
    25441 gccctgcatc gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc
    25501 acgcaatgtt tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac
    25561 aaagtaataa tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt
    25621 cagcagcgag agtttcgtct gattcaatgt cgccatcagc tgaccgttgg cgagagccaa
    25681 atcgtacagc ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag
    25741 ctcaagataa cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac
    25801 cgggaacagc agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag
    25861 atacatcggc tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatagc
    25921 gatcagcaat gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat
    25981 gaagccgctg gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa
    26041 ttctactgag gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg
    26101 tcccgccagt cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgtcagcgg
    26161 aatcgtcagt tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat
    26221 aaaaatacgc gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact
    26281 tagcatatag gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc
    26341 agcttgcgct gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc
    26401 aattttaaag ttcgcaatac cgacaggaat gccaatgatg gaaatacatt gtgcgatgcc
    26461 cgttgcaatg tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa
    26521 taccgtaccg ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg
    26581 aatagcttca ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt
    26641 cagcggtaag gtaaaaatca gcacaatact gaccagagtc gccaacagcc agcccagagt
    26701 ggtggcaaat ccgccaagca caaagtttag aatgttcaaa acggtacgca taaaacctcg
    26761 ctttactgtg gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt
    26821 tttctctgac ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac
    26881 tgaaagcgac aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc
    26941 tcaatgccgg gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat
    27001 tactggcgat tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg
    27061 acgaaaaagt ggtgcgtctg cacggtaccg aatggggcga gacgcagcgt ttttaccatc
    27121 atcttgatgc tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggtgttt
    27181 tacgccagca actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg
    27241 agcaaacctc tggcgttcag caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg
    27301 ttaaccgact ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt
    27361 tgaaagatat tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta
    27421 ccgagtatgc ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc
    27481 gggcagtcgt taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa
    27541 cgtcggtgct ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc
    27601 aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg
    27661 aacggctaca taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta
    27721 ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc
    27781 gtcatgaact ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga
    27841 agggctggcg gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct
    27901 gggatgatga aaaattacag cgtcgtcttg cctcacgcct cgatcgttgg gtaagtctga
    27961 tgcggatgca cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg
    28021 atctgttcag taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc
    28081 tgaaggcaga aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg
    28141 agaaaggtcg ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct
    28201 cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt
    28261 tcgccgttgg tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca
    28321 ccaccgcttt ccatgaaaac tttggtgaag gcgaacgctg tgatttagac acgacttacc
    28381 gttttaacag tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagc
    28441 tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg
    28501 agagtcaact tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca
    28561 ttctgatcct ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac
    28621 gctggccgaa gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg
    28681 attacgtcat catcgttggc ttgcaggagg gaagtgatgg ttttccggct gcggcgcggg
    28741 agtcgattat ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac
    28801 ggcggttaat gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca
    28861 aagagaatcc ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtggcgagaa
    28921 aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag
    28981 ataacgctga taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat
    29041 aatgaagtct gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg
    29101 cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt agtggatccc agaagaaaat
    29161 caatacatca attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg
    29221 gccactcaac atcgcgttga cgttcatgcc ggttgcgcgg gaaatcaagt taccggtagt
    29281 gcctgttgca tacagtacgt gttgttccag taacggttga tgtcgttcca cccagctcat
    29341 cagcatttgt ttgcagtgat cgtgtgctac cagcgcaata tgtttccgcg caggtaaagt
    29401 gcgagtcgtc agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc
    29461 acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa
    29521 gatggttgtt tttccgccca ttgcaggaaa gtatttcgcg tttgtgggtc agccagttta
    29581 aaccaatact tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc
    29641 tcggaaacac ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt
    29701 gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt
    29761 gccgttgagg taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc
    29821 aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga
    29881 ggcaggcgaa aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg
    29941 gagatataca gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact
    30001 aactgatgcg gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa
    30061 accttcttgc cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca
    30121 aaaacggata ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt
    30181 agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt
    30241 actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag ctgcatggtt
    30301 ggcatgtcgg ctttgtcgta cactttgtga aacagtctgg aggaaataat gaaagaaacc
    30361 gatattgcag gcattttaac gtccactcat accattgcgc tggtgggggc aagcgacaaa
    30421 cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt
    30481 ccggtttcgc caaaagttgc cggcaaaacg ctgctaggcc agcaggggta tggcacgctg
    30541 gcagacgttc cggaaaaggt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg
    30601 ggcgtggcac aggaagctat tgccattggc gcgaaaaccc tctggatgca actgggggtg
    30661 attaatgagc aagcggcagt actggcacgg gatgccgggt taaatgtagt gatggatcgc
    30721 tgcccggcta ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa
    30781 aaaccttccg gggatttgtt cagggattag ttacgcagac gcggggcctg gagttgtttg
    30841 cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg
    30901 ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc
    30961 accacgtgat accacggagc ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac
    31021 ggttcagaga gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg
    31081 gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa
    31141 atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca
    31201 aaccctttca gatatagccc ttccggatag gtagcgatca ccggatgatc ggctgcctga
    31261 cggaactgct ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt
    31321 ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgcct
    31381 tcattcagca actgaatcgc cagcatgttg atgtctttat agccacgaca cgcgcccatc
    31441 aactggcttt tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca
    31501 ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg
    31561 ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagggc ttcctgggag
    31621 gtatcaacgc tgacaacctg gttgcaaccg cccatcagtg ccgatacggc gaaaccaccg
    31681 gtataggaga aacagttcag tacgcgttta ttttcaacgt agcggcgggt agccaggcgg
    31741 ctgtcacgct ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc taccagcagc
    31801 ttcatgccat gttcttcaat cggcagcagg gcaggcggca actcgccggt gacgaggccc
    31861 tgggtcagct ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca
    31921 cattccgggt acagcgtttg cagggcgcta attaatgccg cgcgctgata ctctgcccca
    31981 gcactcagca gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg
    32041 ccatcagatt ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag
    32101 tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca
    32161 gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt
    32221 gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct
    32281 tccatacggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg
    32341 cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa
    32401 tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga
    32461 gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga
    32521 tttcgctaca ccacacagta cgaagcgaaa agactgggct taaccgggta cgccaaaaat
    32581 cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt agaaaaatta
    32641 atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag
    32701 ccgcatcatc cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt
    32761 tttggcaggc cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat
    32821 aaatagcggc tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata
    32881 cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc
    32941 cagtgttctg gcgacagcga aatcccttcg ttctctgcaa tcaccactgc cagcggctca
    33001 ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg
    33061 aagatcagca taattattac taccttaatc agactgccgg cagtgtaaca aaaaacaaag
    33121 ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag
    33181 cccagaatgc tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg
    33241 gcacgaatat agttcgtttc accgccatga atgatgttgc tggtttcaaa caaaatagcg
    33301 ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat gcagagcagg cagttgcagg
    33361 aagatattag caaccatacc aatcagcacc accacaatac ccgccatcag cataccgccg
    33421 aggaacgaca tatctttgcg ggtggtcagc acatatgcag agcaacagaa gaacactaac
    33481 gccgttccgc ccagtgccat agcgattacg tcacccattc cggcagacag ataggtattc
    33541 agaattggtc cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg
    33601 gtcggcttat tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc
    33661 agaatcagac ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa
    33721 aaggccagcg tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc
    33781 agtgatgtac ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca
    33841 gatgtaatta attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat
    33901 ctttacgcat ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat
    33961 aacgccctga gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta
    34021 tcttatctgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc
    34081 aagaccgatg aaaaataaat aaaaatcaat gcaatactaa aaacagctga aaaattaacc
    34141 aaacttaaca cctagaaaaa tcaggctgtt agggagcttt cagagttagt ataccaagtc
    34201 ttttttgcca gaaaatttcc tgagttgcct acttgctgac cacaagtggc aaatccagtg
    34261 tcggcgtgat ttttgtttta cgatcataaa tcaacacctg attttctgtt ttatgtccac
    34321 tgaaaatctg tttgtcgcga ctgcttcctt cgtaatctga tattcctttg gcttttatgt
    34381 catggaaatt gcacccaaac gggacgccgg attttttctc ggctgcgtgt ttagcctggt
    34441 tccaccagtt gtttagcgtt ttagctatga ccttcccgcc cttggttgtg ttgatcacat
    34501 attcacatgt gcctgaagat acatttctgg ctaactggat cgcggttcgt aatcgtggag
    34561 accattcctt gatttgtttg gtgccggtct tgttttgctc aatgtagatt cctttatcca
    34621 taatatcctg ccatttcagc tcgagcacat caccgagtct tgccgcacaa agataggata
    34681 tctccattgc aatgcgtaac tgtggtattg cttccgcata tatcgccgca tactgttcat
    34741 ctgtgatgta aacagtgcgg gctttaagcg taaatttcct gactcctttg catgggttat
    34801 tcttcacata tccacgctca tatccccacc cgtatacacg actgagactt gccagttcat
    34861 ggtttgcctg ggtcttgctc tcaatccccc gtttatccat gaaaattctt acctgctcaa
    34921 tttttacatt atcagccagt acttttccga agactgccag taacgccctc tgatgttgcc
    34981 gataatcttt ttgggttctg ggggccagtt ctgtaaatgc gggggaatcc ataaacatgt
    35041 gccataattt agcgacggtc attatgttgt ggcgttttgc tttttccagt tcataattct
    35101 gccagacttt agctatgctg gtttcccgca ctcttcctag ccctatagtt tttgtgcttc
    35161 cttcgggttt ccatacgtaa ctgtaaccat tcgatctaac acgtggcggt agtgcattat
    35221 cttttttgtt ttttcttggt cttcccattg ttcagcgcct caaaatcggg gtcagcagaa
    35281 accagttcag atactttcgg tatcgcagtc agtccgcgcg ggacatcact gcgaagaact
    35341 attggttcgt ttttaggacc gggtacaaat gggataccgt gcaaccttaa ctgttgttgc
    35401 tgttttgtat accgttcgta tctcgtgatc tcttgaattt ctgctggcga tagagttaat
    35461 tcgtacatgt ggtcacgtgc cttacagcat gaccgccgcc actatagctt gtgggcggcg
    35521 atatgggttg aacattaaaa atcagcctga ttcgggaaca gtttttgcca gatagctgaa
    35581 acgtattttg cctggtaacg cgcatcgtgc agggcattat ggcgttcacc ctcaaatgtg
    35641 atgacacttc gagcatcgaa atccatcacc agccccaggg caacaatcgt tcttacatca
    35701 cgatcattgg tgtaacgcca cgggcagggg atctcctgcc gttcatatga acggcgtaaa
    35761 atcacgttgt cgaagttggc tccatttccc cagacctgaa caaaaaactc accggagttt
    35821 tcgtctataa attcccgtaa ttgtaaaagc gcatcatcaa gcggtattcg atcagctaaa
    35881 atggcggcac gggcttcgcc agagcgttga agccaccatt cgatggtgct tatgtctaca
    35941 gtgccgccac atgtacccat atcaattatt ttgctgaatt caggcccctg ctctccggtt
    36001 tgtggatcga aaaatacagc gcctattgag gctattggtg cgtcaggatt tttgcccatt
    36061 gtttcaaggt caatcatcag atggtgccac aacctgctgg tggatgtgat ttcacgatga
    36121 ccgttcacct taattaaggg atttgctgtc tcgcctgttt cattattgcc attggcatgt
    36181 tgatcgcctt cggtgcactc cttgttcggg agtccggcac tttccatttc ctccggatct
    36241 ttttcccggg cttcatcctg gctttcttcg ttgaatgtct cctggtaggt tgcgtccccc
    36301 atcactgcgc cacaatcagg gcagttacca ccaccagtct gattgcatgc ggtacaaact
    36361 ttttcctctt cctgttgcac tactggttca ggttgtttcg tttctggctc gttttgtaac
    36421 gcatttgggc tgttttgttc cgctttctga ccgttctgtt ccgtttcttg ctggttctgg
    36481 ttcacagaat cgcaggtttc aatcccctta acccatttcg aatcattcgg gtcgctaatc
    36541 ccttcaacaa actcaccacg agaagcggca agcaatttgt cggcatctgg tcgactgatg
    36601 tctgctgcct ccataatttt atttacttct tcagcagtaa cttttacctg tgacttgtca
    36661 tccagcggct gcgggtcctg atgatgttca actgtatccg ctgccattgt ttcagccgtt
    36721 gcctgttcat ctgccattgc gccagatggt tgcgggtttt cttcatcatt tttttcttct
    36781 tctgttttgc acttagcagc cagtccgccg ttaatttctt ccaggatatc tttttccggt
    36841 gtatgccggg cagccatgag cgtttcagct gtgggattct cgtgatcagt ttctgtcagg
    36901 ttggcattga tatacccctg aaggcgtccc gggtagtgat aaaactcagg gtgggcgctc
    36961 cggataagtg caaaaatagc tgcgcgggaa tagtccagga taccaggggt tgcgcgaagt
    37021 gctgcggacc attccctgaa cggactttct tttttcagga cgatttcttt tgcgcgacga
    37081 taaacgctca cgggtatttc ataaatatta aaatccatcg gaagtgtggc tgctgcagcc
    37141 tccacatcca gagtatcgag ggtgtgtact aagtcaggat tacgatcggt tttatttccg
    37201 ccaccggcat ttgcaccgga agccgtgcgg gtgatgcgag aaacacgatt tcctttcatc
    37261 cactcttttg tcagcagacc tcgatcggta tagtcagcgt ccaggtatgc ttcgaaaaaa
    37321 gcagtcatta accccagact ggaattaacg ggattaggga aaactttgtc tgtgtcgcgt
    37381 accagtttgt gaaggtcgcg aatctccagc gggtcgagca gctttgtgtt gtgggaaata
    37441 gccagggcag taacagccgg cagttcttca gctcgtgcga tatgtaatgc ctgaagttct
    37501 tcccgtgaaa cgtgcgttac cggtttttcg ctgccatgtt gcgcaagcca gcgaatgggc
    37561 agttcctgac cggaaatcgg caggagcata ttatcctcaa tctcagccat gtcttcgcca
    37621 ttgacgttgg tattgtcagt gctggctggt ttgtcctgaa ctgaggggga aggcgctata
    37681 aatatcattg tgatgccatc ttccccgcct ttttcatagc ggttgcagaa ttcggtatca
    37741 aacacgcctt ccggtggaag gtcattcaca acggggaaat ggacgcggac aggttttttg
    37801 aaatcatcag cgtcgtagcc tgcctcgtcc attgcaacaa cgcaccgtga gatggcaaca
    37861 gataattttt ttgctgtagt ccagtaaaaa cctcctttaa tcccaaggcg ttttctgacc
    37921 ttgtcatttt ttgcttcgca atatagcgaa aattcttctt tatcagtgct catggataaa
    37981 cctcataact attttaaggt tgtacgaacc cctgccattg ctggcatgct taatcaacgg
    38041 gtatggcgtt aatatggctg gtggattatc cagccggtat ttcgttattc aggtacagcg
    38101 atactttttt taacgggagg cattcgccag aaattttttg ctcgtctctt gcacggaggc
    38161 atgattcttt actggcataa attccggtaa tcacattctg tggctcaccc gttataagaa
    38221 aaaccgtcat caccagtgca aatgcagaag tcactgctgc cctccgaaaa taccaagttt
    38281 aagaagggca attctggaga gtatggaatt atcattgaga agataaggct catattttct
    38341 catcttaatg gcatcttcag taaactcccg gttactgagc agaataccaa tatcaaaaca
    38401 cccttcagac gtattaacgt ttggtaataa cgtttccatt atcgcgtcct caacaatgaa
    38461 tttttagtaa ttgctccaca gtcatatttt taattgcgct ccggtttaca agagtccagc
    38521 cttgtttctc cagataaaac tggaaggtat ccagggtgca gaccattgca ccatcaggaa
    38581 cggtttcagt gaatttgata ttgccgtgtt cgtcgagacg gataaccagg gtacgtccgt
    38641 ccccgtgaat cattttgtcg ggagacgggg cgttattctg gcgcagttct ttctccatgc
    38701 gatcgaactc agcgatgtag gcttctttaa atgcagcggc ttttttgccg gtgaagccca
    38761 tcaccaggaa aacgaagccg tttttggtga tttggtacat tgggagtttg cgaccggttg
    38821 agtcggtata ttcgctcgac acaaaattgt gctcagtgaa ttttgctgaa cagtccagat
    38881 tgcgaatttt atccaacact cgttcgtggc gtttaccaaa gaactcagcg atcgcaatag
    38941 acgtagtgac agcgcgtcca ttttcgatgg ttacgtcagg gtgagaaagg gcagggatag
    39001 tagccatgat ggcagcctcg atagtcagtt taaataactc accaccaagg ctttccacga
    39061 ccataagggt ggtgagacgt acaggggtgg aaataccggt gactaccgaa accggccagc
    39121 ctttcggctg ccctgcacgc cccaccataa tctgaatgtg gctgtgcatt acgcataaaa
    39181 aaaccgcctg agcgcggtta tgcgcagtag tcatcatcgg gtttccacgc ccggcacccg
    39241 ctttatgagg tgcaggtgca ctataattcc acccgttctg gttttcaata gctacattca
    39301 acattttctc ttacccttca tcactgaagt gaactttgtt gatgcagtgc ctggtgcctc
    39361 caggtgacgt taaccagtta acaattaacg ccggaataca gaaggatgcc cgttacgccc
    39421 cgtaaaagac cactttacgg ttttaactgt tccgcgtgcg cttagccgca ttcaccgcat
    39481 cacaaaattc actttaaaaa gggcggacat cagaaagcta ataaactgat gccgccaaag
    39541 gtaccactgc cattgaaaca tggagatgtt gtggcggggt tgtcactcag gcatatggtc
    39601 aacctgacaa cccggtgtcc tcaatgaggg gaaaagaaat aaaaaccccc agccatattt
    39661 accgccgcgc catttcgcgg attgccacaa ccggaagcac acggtcgaag aaatctaacg
    39721 acaagccttc taaggaaaag acgccttcgc cgtgtgcttt ctgttgtgcc ctgacttttc
    39781 agggtaaatt aaccagtggc aacctgtttt tactggtgct aatcagttag tgtttctggc
    39841 taaccagcga cgcgcgccag cttcggtttt aaacttttta cttttggtat acgtcatcgc
    39901 ggtaaacgtg ccgtcctggt tagggaacac accgcatacc agagattcgt tgttgccaag
    39961 attgagcgta tccatgttga cctcatttcc ccttaacgcc ggggtagcgg aacaaaaaac
    40021 ctgctgcata gttaaacgta atccccgccg tcatgttcat acgcctcagg ctggctactt
    40081 aacccctgac cactgccggg taactcgaag tattgccctg cgttctgtgg ggcggggtgg
    40141 gtttatggat acaatttaca aattaaaatt taactggtca acatgtttat tattaaattt
    40201 gtaattgcga gctgttgcat cagtcccaaa atgggactac tatacagtta tgaagattat
    40261 ctcagttaaa acactcaggg atttttgggc ggagaatcct gatgcagaac aaccgctaag
    40321 ggcatgggtg gatgaggcgg caaaagctga ctggaaaagc ccagcagaca ttaaggcaca
    40381 ataccgaacg gccagtatat tgaaaaaccg gcgcgtggta ttcaacataa aaggtaatca
    40441 ttaccgttta attgttgcaa ttgcgtatca gcgaggatgg gtatttgtta aattcatcgg
    40501 aaaccacaag gaatacgatg ctattgatgc tgataccatc gaactggagt aagcatgaac
    40561 atcaaaccta ttcgtacaga gcaagattat gaagccgcgt tgcgtgctgt tgaacccatg
    40621 ttcgacaatg agcccgaaat tgacacgcct gagggggatt tttttgaggt gatgtgtttg
    40681 ctaatagagg aatatgaaaa aaaacattat cccattgagc caccatcccc aattgaagct
    40741 ataagatttc gcatggaaca gcaggggctg actgtgaaag atttggaacc cgcaattggg
    40801 aaaaaaaatc gggtttatga ggtgttgaat ggtaccagaa gccttacgtt accaatgatt
    40861 cgccgtcttc ataatcaatt tggtatcccc ctggaaagcc tggttggatt ataaaatctg
    40921 ctagtcattt gcctgatgct cgttccagaa aaggaatgca tcaggcagtt ttgtttttct
    40981 gccgcagtaa ctcttcaagt ttccgtttat agaaatcgcg tttttgctcc atatcacgaa
    41041 tgatctgctc tgcgtcgctt tgaggtaact catctaaaag cgatatgatt tttcgttgtt
    41101 gttctgtaag ttgcggttgg ttgtcattac tggatacagc cattttatcg ccgagagttt
    41161 cttcttccat aaagaaccaa tggacgggat gttgtgagag ctctgctaat ttttccagtt
    41221 tatccattct tggcatcacg cctttcaacc aaccttgcac ggattggggt tttacaccaa
    41281 gacgtcttcc cagctctgac tggtttatat tcaattcctg caacacctgc tgaaggcgtt
    41341 ttacaaagat catcaccacc cctcgtaaac tagttccgcg atcctacaga aaaaattgat
    41401 aagtggcatt acaaatagaa gttgaaattt aaatttaaat ttgtaatcat cggtgtcatc
    41461 gtaaagttcg gagggaaaca tgcaaaaaag tactcaagtg aaaatcctgt caataatgag
    41521 ccaatcagaa ttagggcgtc gtcttggtaa aacaccgcaa accataagtg ggtggtttaa
    41581 aaaacgagtg ccagcggagg aggttatacc agcatgtgag gcgcttgact ggggagtaac
    41641 tccgcatgaa ttgcgccctg ataaataccc taatccaacc gatggtttac ctgttgagta
    41701 tcaggctaac gaacaagcag cggcgggagt tgattcatga aaatcaagca tgagcacatc
    41761 cgcatggcga tgaatgcgtg gctgctttat ccgagggtag ggcgcaaaaa aatcgctgat
    41821 gatatagcga cagcatattt tgagcttgaa atgacttatc caccaatgca tgacacctct
    41881 acgacagagg gtattggatt gaatatacaa aatatttttc gctggcttga aaaggatacg
    41941 cctgatgctg ttgaaaaaat tcaggcacta attccggcta tcctgactgt tcttccgcgt
    42001 gaactgcgtt atcacctcag tatttttgac actgttgagc gccgtgcatt actggcggcg
    42061 caggaagcgt tgagcacggc aattgatgca catgatgatg ctgtccaggc cgtttaccgg
    42121 aaagcgtatt tcagcgacgg cgggtcatcc ggcgagtctg ttgtggtgca ttgatattta
    42181 tgccagcccc ctgctgattc tgttgattgg ggaatcacag aatatcacca gaggatggtt
    42241 cgtcacaaga tgaggcaatt atggccgcat taccatacat gcaactgtac atagctgatt
    42301 acctagctga caccatgcat ttgtccgcag aggagcacgg tgcgtatttg ttgctgatgt
    42361 tcaattactg gcaaacaggg aaaccaatac ctaaaaacag gctggcaaaa atttcccgtc
    42421 tgactaacga gcgatgggct gatgtggaac catccttgcg ggagtttttt tgcgataacg
    42481 gcgatgaatg ggtgcatctt cggattgagg aagatctggc atcagtcagg gaaaaattaa
    42541 ccaaaaaatc agccgccgga aaagcatctg ttcaggccag aagaagcaga aaggaagcat
    42601 atgttcaaac aaaacaagag agagatttaa caggcgttca aacaaatgtt ggtgttgtgt
    42661 ttgaacatga cgcaaacaca aaagcaacta ataaagatac agatctaaaa gaattaaacc
    42721 ccacacataa cgtgcgtgaa cgcgagagta ttccgaccag tgagtcgcat ggtgcgccgt
    42781 tgcagacagc cgaacctgaa tacctggacg gcctgagcga accgatcggg aaattttcga
    42841 tgaccactgt ctggcagccg tcgccggatt ttcgacaacg ggcagcagtg tggggtatgg
    42901 ctctgcctga gccggaattt acacctgctg agcttgccgc attccgggat tactggatgg
    42961 cggagaggaa ggttttcaca caggttcagt gggagcagaa atttgcccgc cacgtgcagc
    43021 acgtcagggc acaggtaaaa ccagtcagca aggggggaag ccatgcagca tcaggtggca
    43081 cggcatcacg ggcagttcag gaaatccggg cagcacgcga acagtgggaa cgagacaacg
    43141 gatttatcag caacggaaac ggcctggaag ctgtgggagc ttatggggga ggtgtattcg
    43201 aaccgctgga ctcagaagaa cggggccgca ccttcgaagc tctggattgc ccagattggt
    43261 gcgatgactg aacagcaaat ccggcaggtc tgccgtcagt gcatggaccg ctgccgggcg
    43321 ggtgaaacgt ggcccccgga cctggctgag tttgttgcac tgatttcgga gagtggggca
    43381 aatccatttg gtcttacggt ggatgcagtg atggaagagt accggcgctg gcgcaatgaa
    43441 tcctggcgat acgacgggag tgataaatac ccgtggccac agcctgtgct gtaccacatc
    43501 tgcctcgaaa tgcgtaccag agggattgag cgccagatga cgcagggtga gttaaaacga
    43561 cttgcggaac ggcaactgac gaaatgggca aagcatgttg gtaacgggat gagtgttccg
    43621 ccagtgcgac gacaactgga aggggcgaaa tacccgaaag ggccaacgcc aattgaatgg
    43681 ctgaaacagg aatacgaacg ccggaaggca gctggtttta tttgaatctg agaagcgatt
    43741 ttgtaggagg aaattttaat ggaaaccgtt tttgacgcac tgaaagcaat gggaaaagcc
    43801 agctctgttg gactggctga gcggcttgat atcagtcgtg aagaggttct caacgaactg
    43861 tgggaactca aaagaaatgg cgttgttgat aaagcgggtc acatctggtt tctggctggc
    43921 gaaggtgaat ccggggtaac cgaagagcag ccagaacagt ctgaagtacc ggatgtgctg
    43981 accggggagg tcgaacaaaa agttaccgca gacatgatga ttgagtttat cggtcaggag
    44041 ggggctaaaa cgtgtgagga actggcggat aagttcggtg ttagcattcg caaggttgct
    44101 tccacgttgg cggtggtaac cgcaacggga cgcctggcac gcgtaaatca gaacggtaaa
    44161 tttcgttact gcatgccggg cgataattta ccagcagagc cgaaagccgt gccggtaacg
    44221 gaaaatgatg gtaaggcctt tcctcagcca gcaggtgttg cgttaccagt acaggaatct
    44281 gcaacacagg aagatattaa aacagaaaca gtggcggaca ttgtgcagtc gttgccatcg
    44341 tttaccgaaa cgcaagcaga tgagctgatt tttctgtccc tgtgcagggc aaacctggcg
    44401 ctgcgcaggg cgaaaagtga tgttcagaag tgggagcgag tctgtgccgc gctgcgggaa
    44461 ctgaacaaac acagggatat tctccgggat attaccgcca ccagagagct gctgcggtga
    44521 gtggctggaa gaagtggcgc tgggcggaaa ttatgatact ccggcagtgt gcgggaacga
    44581 tgacagtcga aagcatcggc agtctgattg gccgtagtgg ggctgccgtc aggacgaaag
    44641 cgcgggaact gggtatctgc atgatgttac ggggtgatta tcaccagtca gccaaatgtt
    44701 cacagcgtga tattgagctg gcgcggcaac tgcatcagcg tggcgtaccc cgacgggaaa
    44761 ttgccgaaaa gtttgggatg aagttgggcg cagtgaataa ctacgtttat ttcgacagga
    44821 gggttcaggc gtgagggtga gaatttatat cgccggtccg atgacgggat atgaaaattt
    44881 caaccgtgag gcgtttcaca gggcggaaga agcactgaaa cgggaagggc ataccgtttt
    44941 aaacccggca gtacttccgg acggactgac acagccacac tacatggata tttgcatggc
    45001 gatgatacgc tgcgtggatg cgatttacat gctgaaaggc tggcagcggt cggcaggcgc
    45061 taaggcagaa ctggcactgg cggagaaact gggacatgcg gttattttcc aggaggcaac
    45121 cagtgagaaa aattaattat cagacgctac gtgagatagc aaaactggca acccagggcg
    45181 aatgggtcgc attcatttcg ccgggcactg atacatatgc ggtgcatacg ccgggggatg
    45241 aacgttgcgg agacattatc aaatggcctg gctttgatga ccagaaaaat gcagagaaca
    45301 acgcagaatt tattgcagct ttcaaccctg aagtagtaca ggcactgctg gatgaacggg
    45361 aagcccaaag caaacgtatt gcagagctgg aagctaatct tgtggcgctg gcggcggaga
    45421 atgcacttgc tcgtaaagca gttcaggcat tctgcgatgt tgttggcgac aacaccgagg
    45481 ttatctgcga ggaggttggg agagatggcg ttctggttat tttggaggcc atgaaggcaa
    45541 caggaaatat gccagccacc gatgctttcc tgtctgaagt gcgggcgctg ggggtggatg
    45601 ctgctataga agctgcaaaa aatctggtgg cccaagaata tgagtataag gatttcaaag
    45661 cggcgcagag tgattgctgt atgcaccctg gttcagacct gatagggaag gttgaaatga
    45721 ctgagtggtt agttgacttt gctgcccagc ttcgcaaagg agttcagtca tgagagcttc
    45781 aattagttat gtcgacgatt gccacctaag cgtgcgtgtt gatgaaattg tgtcatccgt
    45841 gccaactttt ccgacaaaaa atgcagccgt taatgcaggc gcgccattcg gatggaggac
    45901 ggcagttcgt attgagcgtc gattcgaaaa cgtttgggtg gtaggtaaga aatgcttcca
    45961 aagcgatcgc tctgcggggc ttaattttga agcatatcga ttcccgttcc tccgatggga
    46021 gaaagagggc gggattacaa aatgcccgat tctttccgtg cgcagattca agcaggaggc
    46081 aaccagtgag caagattgac tatcaggcat tgcgtgaggc agcacaaaac tatcggtcga
    46141 tgctggcgtg gtatcaggag aaaccagaca gtccaaacgc tgagcaggat tgtgatgcag
    46201 ctttggctgc gtttaagcgt gaaatccgtc atcgtgaagt gggtattatc gctgatttgc
    46261 tggatgaact ggaggaagca aaacagcgta tcgacgagca ggagtcccgc atagtgaagt
    46321 taccagaacc attcaagctg gctaaatcat caagtggatt aacgtactac tacgctgatg
    46381 aggtcaatgc cgcactgact gctgctggta ttcgcataga aggagagtga gatgaccact
    46441 attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga cattgacgat
    46501 atagacggcg gtgaaattcg tgagctggcg cttatcgcgc tggcatcgct ggaagcgaag
    46561 cctgtagctt catgtattat tgaagatggg ggcatgtgtg ttgacgggtt cggtgagtat
    46621 gttggtcact cgctgcctga tggaacgcac cagctttacg ccgccccgcc agtgcctagt
    46681 aagttgccgc gtgaatacag aaacggttgg cctcttgcat atagtgatta tgctgaaggc
    46741 tggaacgact gccgcgaagc catgcttcag ggagataaat catgattaat cgtatcaagc
    46801 tggagcacat cctcgaatat gccaggcagc agaagcatac tggtcagcat tgtaaaattc
    46861 caccagggga tatggttgaa atcatggaga ttgccatgcg caaggctggc aattctccgg
    46921 taactccgga tggttggata agctgtagtg agcgaatgcc ggataccaaa acagccgttc
    46981 ttgttgccag ggagtttgac aggaaaggtg actggcgaat gaaatgggcg acttacatcc
    47041 cggggcatcc tgacgctaat gatgggtggg tagtacctgg tgcgtcgtgg ataccatcac
    47101 actggatgcc gctaccagaa ccgccgaagg aggtgcgcca atgagctggc ctgaggcctt
    47161 ctcaaatgtt ggtattgcaa tggcggtggc gctggtgggg tattcgattt gccgctgggg
    47221 ataaatcgcc gaaaaaagat cccgccacaa acatgagccg ggatctttga tttatatagc
    47281 ctacgaatcc gcagtaagag aggggcagac ggtttattct aacaccggaa tgatgtgggt
    47341 aaaagtttat aagaaatcgg tttcataact ttgcccacca tgatagatac cgacaataaa
    47401 gacttttctg ccatcaacgg caaaagcaat aatcgttctg tggcggaaat gagttacccg
    47461 catcccctgg cgaatatcat cgcgtttatt gccccgatgc gggaatgtag aaaacccatc
    47521 aagataatca agaagcgcat tggcataatt gtcagcaatg atgttccctg ctttctccgt
    47581 tatatatctg tgcaggttga ttatttgttg ttcggcctca ggagtaatga tgacttcata
    47641 tgtcatgcag attacttccc ggatcgaatc gcgacgcgaa cctgtgaaat ggagcgtccg
    47701 ttgtttgggt tttcgcggat agaatcaaga gagggggcgg ctgaatgcgt taaccacgct
    47761 tcgattgctt tatcgcgctc attcagtgcg cgaagccctt cacgaatgac ctcgctttct
    47821 gaagcatagg cgccggaagc cacacgggcg cgcaccatgt cagccatctc gttagttaat
    47881 gtaatgctga attgttgggt tgtacgcatg gtaaacctca cggagtagga tagaacacta
    47941 ttcgatgata gcacgctgcc tgttgacgac aacagaaatc agagacaata ttgccgcacg
    48001 ccagcctgaa caactggcac ctgctgcgcc agcagagaca accgatggcg caagatacca
    48061 aattacacaa ttcggataac tccaccgccc ctgccagcag gcacgggcgg cgttctcatg
    48121 cattcaaatc tgactggttc cagcatgacc catgcactga agaacaggcc gaatggctga
    48181 tccagtgcta ccgcagacgt ggttacgagt ttcagaaaga tctcagcttc gatcgtcgtc
    48241 actggataat ctccgtcagg ctcccttatt ccgaacgccc accgcgtccg tcccgcacat
    48301 tccagcagcg catctggagg taacgtgcgg gtattacttc gacctgttct ggtaccggaa
    48361 ctcgggatgg tgctccttaa gccgggccgt gaatcaatgt cagcattcca taacggcaga
    48421 atattggtgg aaccggaacc gaaaaacatg cgcggtctgc cgtccggagt cgttcctgcc
    48481 gttcgccagc cgctggcaga ggataaaaca ttactgccat ttttcagcga tgagcgggtt
    48541 attcgtgcag caggtggtgc aggtgcactg tctgactggt tattacgtca cgtgaaatcc
    48601 tgccagtggc cacacggtga ttatcatcac agcgaaaccg tcattcaccg ttacggtacc
    48661 ggcgcgatgg tgttgtgctg gcactgtgac aaccagctgc gcgaccagac atcagaatca
    48721 ctcgatcaac ttgctcagca gaatctggtt gcctggatga ttgacgtcat ccgtcacgca
    48781 ataagcagta cgcaggagag ggagttatcg ctggccgaat tatcctggtg ggcggtctgc
    48841 aatcaggtgg tggatgcact acctgaggca gtagtgcgtc gttcgctggg attaccagcg
    48901 gaaaaaatcc gctccgtata ccgtgagagc gacatcatac cgggagagca ggccgccacc
    48961 agcatactga agcagcgcac aaaaaatatt gcgctgccac ttcacgtcca ccagcaacaa
    49021 aatccccccc aggaaaagac gttagtaagc atcgccgttg atccggagtc tccggctcag
    49081 tacctccagc gccagaaacc acaacgggaa gagatgcctg tatacacgcg ctgggtaaaa
    49141 acgcagaaat gcatgacgtg tggcaatcag gcagatgatc cgcatcacat cattggtcat
    49201 ggactgggag gtatgggaac aaaggctgac gatttgtttg ttattccgtt atgccgtaaa
    49261 tgccataacg aactacacgc cggggtaaaa gattttgaag agaaacacgg cagtcagctg
    49321 ttgttgctga ttcgtttttt aatgcacgcg agaaattcgg gtgttctgaa gtggaaagca
    49381 taaatgaccg agcgcataga atttgttttg ccttacccgc cgacggtgaa cacttactgg
    49441 cgacgccgtg gcagcacata ttttatatcg gaggagggaa agcgctatcg ccgggcagtg
    49501 gcgcttattg ttcgccagca gcggctgaaa ttaagcctgt ccggacggct ggcaataaaa
    49561 attattgcag agccaccgga taagcgtcgt cgtgacctgg acaatattct gaaagcaccg
    49621 ctggatgcgc tgacgcatgc gggagtgctt attgatgacg agcagtttga tgaaatcaat
    49681 attgtacgtg gcaagccagt atctggtgga cggctggggg tgaagattta ccccataatg
    49741 cttgaagggc aggtcaaaaa atgaaactgg aagatttacc gaaatactac tccccaaaat
    49801 cccccggcct gactgatgca tcggcctcaa cgtcgaaaga tgcgctgagt atcactgatg
    49861 tgatggccgc gcagggcatg acacagaatc gggctgagat ggggttttct gcgttccttg
    49921 ggaaaatggg cattagtatg aatgacagag aacgggcaac agaattgctg acagaatatg
    49981 cactcagtcg gtgtgattgc gtggcggcgt taagaaaact cccggcagaa ataaaaccgg
    50041 cagtgatgcg tattatggct tcgtatgcgt ttgaagatta tgcccgtagc gcggcgagca
    50101 aaaaacagtg ctcctgttgt catggaaaaa aatttattga aagcgaggtt tttacaaaca
    50161 agatccagta tccggatggt aagccgccag tgtgggcaaa gtgcacaaaa ggcgtgtatc
    50221 cgtcttactg ggaggaatgg aaaaaagtca gggaggtggt aaaagttgcc tgtccggagt
    50281 gtggcggaaa gggggaggtt tccaccgcct gtaaagattg tcgtgggcgc ggtgttgcca
    50341 ttcatcgtga agagtctgaa aaacggggta tgcctgttat cagggactgc cagcgttgtg
    50401 gtggtcgtgg ctatgaaaga ttgccgtcaa cagaggcatt taatgctata tgcgaggtga
    50461 caaaccagat aacacgcgcg tcatgggaaa aaacagttaa gaaattctat gatgcgctgg
    50521 tgagcaagtt tgatattgag gaagcatggg ctgagaggca gttaaaaaag gtaaccaggt
    50581 aacaaggttg atttttccgg aatctgtggt aaatttgccc taacgatggg cgttttatgc
    50641 ctgacgttag aagacttttt acacccgtcg ccaggcgggt ttttttatga ctgaaatcac
    50701 tccagtacag taaacgcgct ggcggcggtg aataccggtc tttcagcttg ctggcttttt
    50761 cgacaagagt tattggtgtg tcacgttaac cggaaaaggg aaaaagacat gctgaaacag
    50821 caggatatga ccgaaaccgc cagagtggtg tttaatgaat taagcgtcac cgaaccggcg
    50881 acagtcgggg agattgcgca gaatatgtac ctttcacgcg aacgctgtca gttaatactg
    50941 acccagcttg ttatggcggg tctggcagac tatcagttcg gttgttacag acgccttcag
    51001 tcctgaaggc ttttttattt gtggtaaatg ggcggctggt gggtgttagg ggcactcacc
    51061 ag
//
LOCUS       DAZGCQ010000026        50394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000026 DAZGCQ010000000
VERSION     DAZGCQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.026"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..447)
                     /locus_tag="ACHK07_001618"
     CDS             complement(<1..447)
                     /locus_tag="ACHK07_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101964.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPAEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYQPYTLTFTRGENNEGILSVESKEGFISQRTINLNEYETAIN"
     gene            complement(911..1789)
                     /locus_tag="ACHK07_001619"
     CDS             complement(911..1789)
                     /locus_tag="ACHK07_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8101965.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            1941..3131
                     /locus_tag="ACHK07_001620"
     CDS             1941..3131
                     /locus_tag="ACHK07_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8101966.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            3212..3862
                     /gene="inaA"
                     /locus_tag="ACHK07_001621"
     CDS             3212..3862
                     /gene="inaA"
                     /locus_tag="ACHK07_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO8101967.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIGGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(3916..4170)
                     /gene="yfaE"
                     /locus_tag="ACHK07_001622"
     CDS             complement(3916..4170)
                     /gene="yfaE"
                     /locus_tag="ACHK07_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO8101968.1"
                     /translation="MARVILRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4170..5300)
                     /gene="nrdB"
                     /locus_tag="ACHK07_001623"
     CDS             complement(4170..5300)
                     /gene="nrdB"
                     /locus_tag="ACHK07_001623"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8101969.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(5534..7819)
                     /gene="nrdA"
                     /locus_tag="ACHK07_001624"
     CDS             complement(5534..7819)
                     /gene="nrdA"
                     /locus_tag="ACHK07_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8101970.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            7947..8090
                     /gene="ypaB"
                     /locus_tag="ACHK07_001625"
     CDS             7947..8090
                     /gene="ypaB"
                     /locus_tag="ACHK07_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO8101971.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            8515..12267
                     /gene="yfaL"
                     /locus_tag="ACHK07_001626"
     CDS             8515..12267
                     /gene="yfaL"
                     /locus_tag="ACHK07_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO8101972.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDTTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIELRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFEHSLTGFQNGTLNIDAGSNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFETGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIEVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFRGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(12408..13130)
                     /gene="ubiG"
                     /locus_tag="ACHK07_001627"
     CDS             complement(12408..13130)
                     /gene="ubiG"
                     /locus_tag="ACHK07_001627"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO8101973.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            13277..15904
                     /gene="gyrA"
                     /locus_tag="ACHK07_001628"
     CDS             13277..15904
                     /gene="gyrA"
                     /locus_tag="ACHK07_001628"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO8101974.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            16053..17741
                     /gene="yfaA"
                     /locus_tag="ACHK07_001629"
     CDS             16053..17741
                     /gene="yfaA"
                     /locus_tag="ACHK07_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO8101975.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DEEQDTEATAIASDLLSGKKRWQVSFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDNSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            17738..18361
                     /gene="yfaT"
                     /locus_tag="ACHK07_001630"
     CDS             17738..18361
                     /gene="yfaT"
                     /locus_tag="ACHK07_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO8101976.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGNAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            18385..22899
                     /locus_tag="ACHK07_001631"
     CDS             18385..22899
                     /locus_tag="ACHK07_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO8101977.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWITL
                     QRLNDTQYEARVPVNSTFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEAPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLTHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            22900..24549
                     /gene="yfaQ"
                     /locus_tag="ACHK07_001632"
     CDS             22900..24549
                     /gene="yfaQ"
                     /locus_tag="ACHK07_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO8101978.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLNEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGDRYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            24554..25330
                     /gene="yfaP"
                     /locus_tag="ACHK07_001633"
     CDS             24554..25330
                     /gene="yfaP"
                     /locus_tag="ACHK07_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO8101979.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            25605..28454
                     /gene="rcsC"
                     /locus_tag="ACHK07_001634"
     CDS             25605..28454
                     /gene="rcsC"
                     /locus_tag="ACHK07_001634"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO8101980.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(28654..29304)
                     /gene="rcsB"
                     /locus_tag="ACHK07_001635"
     CDS             complement(28654..29304)
                     /gene="rcsB"
                     /locus_tag="ACHK07_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO8101981.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(29322..30638)
                     /locus_tag="ACHK07_001636"
     CDS             complement(29322..30638)
                     /locus_tag="ACHK07_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8101982.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKAYNIFLKSNS"
     gene            complement(30672..30926)
                     /gene="xisR"
                     /locus_tag="ACHK07_001637"
     CDS             complement(30672..30926)
                     /gene="xisR"
                     /locus_tag="ACHK07_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8101983.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(30934..31080)
                     /locus_tag="ACHK07_001638"
     CDS             complement(30934..31080)
                     /locus_tag="ACHK07_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101984.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(31101..31343)
                     /locus_tag="ACHK07_001639"
     CDS             complement(31101..31343)
                     /locus_tag="ACHK07_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101985.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(31336..32238)
                     /locus_tag="ACHK07_001640"
     CDS             complement(31336..32238)
                     /locus_tag="ACHK07_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101986.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(32235..32459)
                     /locus_tag="ACHK07_001641"
     CDS             complement(32235..32459)
                     /locus_tag="ACHK07_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101987.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(32476..32838)
                     /locus_tag="ACHK07_001642"
     CDS             complement(32476..32838)
                     /locus_tag="ACHK07_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO8101988.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(32831..33697)
                     /locus_tag="ACHK07_001643"
     CDS             complement(32831..33697)
                     /locus_tag="ACHK07_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO8101989.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(33721..34305)
                     /locus_tag="ACHK07_001644"
     CDS             complement(33721..34305)
                     /locus_tag="ACHK07_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101990.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(34292..34852)
                     /locus_tag="ACHK07_001645"
     CDS             complement(34292..34852)
                     /locus_tag="ACHK07_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101991.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(35260..35622)
                     /locus_tag="ACHK07_001646"
     CDS             complement(35260..35622)
                     /locus_tag="ACHK07_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101992.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(35797..35997)
                     /locus_tag="ACHK07_001647"
     CDS             complement(35797..35997)
                     /locus_tag="ACHK07_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101993.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(35994..36185)
                     /locus_tag="ACHK07_001648"
     CDS             complement(35994..36185)
                     /locus_tag="ACHK07_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101994.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(36187..36564)
                     /locus_tag="ACHK07_001649"
     CDS             complement(36187..36564)
                     /locus_tag="ACHK07_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8101995.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(36650..37309)
                     /locus_tag="ACHK07_001650"
     CDS             complement(36650..37309)
                     /locus_tag="ACHK07_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8101996.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            37649..37942
                     /locus_tag="ACHK07_001651"
     CDS             37649..37942
                     /locus_tag="ACHK07_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101997.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            38185..38553
                     /locus_tag="ACHK07_001652"
     CDS             38185..38553
                     /locus_tag="ACHK07_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8101998.1"
                     /translation="MRAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            38587..40245
                     /locus_tag="ACHK07_001653"
     CDS             38587..40245
                     /locus_tag="ACHK07_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO8101999.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            40247..41215
                     /locus_tag="ACHK07_001654"
     CDS             40247..41215
                     /locus_tag="ACHK07_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO8102000.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            41215..42078
                     /locus_tag="ACHK07_001655"
     CDS             41215..42078
                     /locus_tag="ACHK07_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8102001.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            42157..42582
                     /locus_tag="ACHK07_001656"
     CDS             42157..42582
                     /locus_tag="ACHK07_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8102002.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            42782..42937
                     /locus_tag="ACHK07_001657"
     CDS             42782..42937
                     /locus_tag="ACHK07_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8102003.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCDVRIRTGQTEVAVF
                     VDYESEK"
     gene            43065..45182
                     /locus_tag="ACHK07_001658"
     CDS             43065..45182
                     /locus_tag="ACHK07_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8102004.1"
                     /translation="MCECSKNVDFHKRTILCCQTSDRKSRKSVKSVEPDIADRANNWL
                     KSYGLDYKLEQAPLNNEIDNALDAYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLIEYK
                     GYKDRLIRLGSNGIIENKDARKEWNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGM
                     TGWRDDGTGELHHEIGVWYVSKNNLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLP
                     PEELEKIKASKEEEIDTRLSRLNNDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAP
                     LDKKELTSSTEEDLRDGDIIFRKIRNFLRLKAVPETKREMILRSLQNTLWTENINKPV
                     NGESQLKRVFVKVVDDLGEYYKIGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYV
                     ATLLARLARVNKDSYVWDFATGSAGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAE
                     QLLGLEVLSSIYMLAILNMILMGDGSSNILNKDSLADFDGKYGFGKTGEKFPADAFIL
                     NPPYSAKGNGMIFVQKALSMMDKGYAAVIIQSSAGTGKATEYNKKILKENTLLASIKM
                     PADLFIGKSSVQTYIYVFQVKIPHNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRA
                     KERYQEVVDLVHFGKGCLNIFTEDEYFEGTIDPDSGEDWNQTRPVDARPTLDDFKKTV
                     GDYLAWEVSQLLKKQGENNFAGK"
     gene            45251..45796
                     /locus_tag="ACHK07_001659"
     CDS             45251..45796
                     /locus_tag="ACHK07_001659"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8102005.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEYFISLPVKNNEIDWFFMDNYIEELEAAHIEELEAYLLVSGLADS
                     RTRGLADSRTRGLADSRTDKL"
     gene            45849..46370
                     /locus_tag="ACHK07_001660"
     CDS             45849..46370
                     /locus_tag="ACHK07_001660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8102006.1"
                     /translation="MWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISA
                     FISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKS
                     SCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKK
                     LVAYRQITSEKVV"
     gene            complement(46727..46945)
                     /locus_tag="ACHK07_001661"
     CDS             complement(46727..46945)
                     /locus_tag="ACHK07_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102007.1"
                     /translation="MLIYNVFGRHIGVKREGDHWLVFRADLTERKFSRLYDIVIPDDM
                     TEDEIPGWLSDIFHEAATERHPDVKRIR"
     gene            complement(47176..47273)
                     /locus_tag="ACHK07_001662"
                     /pseudo
     CDS             complement(47176..47273)
                     /locus_tag="ACHK07_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47805..49715
                     /locus_tag="ACHK07_001663"
     CDS             47805..49715
                     /locus_tag="ACHK07_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8102008.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            49855..50037
                     /locus_tag="ACHK07_001664"
     CDS             49855..50037
                     /locus_tag="ACHK07_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8102009.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            50063..50335
                     /locus_tag="ACHK07_001665"
     CDS             50063..50335
                     /locus_tag="ACHK07_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8102010.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT        12595 a        11669 c        13124 g        13006 t
ORIGIN      
        1 gttaattgca gtttcatatt catttaagtt tattgttctt tgtgaaataa acccttcttt
       61 gctttcgaca ctcaaaatac cttcgttgtt ttcacctcgg gtaaaggtaa gggtataggg
      121 ttgataatcc gctgcaaatt catttttata cgttatgaca tgttcgacaa aatgtcttat
      181 tggtacgaaa ccataactgt gaacgtcaat tttttgggtc ttgatactat caattgcatc
      241 tgaggctttc gtgaaaaaag ccaataaatt agctatgctg tctgttgagg ttgataaccc
      301 gtatctactt tgagatattt ttagaaactc atcgcaaaaa aactcaagtt tggcggtttg
      361 ttgtgattct ttgtaaattt ctgctggttg caatttatta ttttgtaaaa tattacagcg
      421 agataagtga atagatgtat ttggcataaa tgattccttg tattgtgagt taacaagata
      481 caatttaatc tttgcagaat atattcacta tcaaatttag ctcattttat tatgaagacc
      541 tttacgcatg ttagaaagta tcattattgt taataaatac ggtaataagt aacaaacaga
      601 tattgataac ttccctgaca agttattagg tatcccactt gaagattgag aatggctcca
      661 gagtaaactg tgtactgttc gccggagtgt cattactctc aacaatcgga tttgacagtg
      721 agtcgggcgc ttcataccgg tttctacctg gagtcattgt cacttctccc aactggataa
      781 atatatcgcc aattcacaaa gtgggccaaa atcaggtgaa aacgaattat tacgcctggt
      841 aaaggctata gtacaactaa gtgtggcgaa tcaggcagtc ttgcatttgt catcaatctc
      901 atgtcttaca tcaaattttt ccactcacca ctagttgttc cgtacacatt tgtagtaaaa
      961 acagtggact gccttctacc ggcttttctt tagccagcaa cgaaatctgt cgatagaagg
     1021 ttggttcgag aggaacggaa cacaattcat ctgcaatggt tttcagcgtt aactctggta
     1081 ataaggcgat gcctaatccc tgtcgaataa aactgatcgc tgtatccggg tgattaaatt
     1141 catattttat ctgtggggta atattctttt ctttaaatag tgccataatg cttaattcat
     1201 agcgtccttt actaatgatt aatggttcac ccattaattc ttccactgta atagtgctgt
     1261 tttttgccag agcatggttg ggcggtaaca ctacggtgaa tttatcctga taaatgggca
     1321 cggagtacat tccgctcacc ggaaaatgaa caaaaccggc atcaatggat tcattctgaa
     1381 gcgaatcaat aatcgctgtg ctattttcct caaaaggaat aatttttata ttcggatgat
     1441 gaatttcaaa gtagcgaatc acaaccggta agatacaggc gcagacgctg gggaaacaac
     1501 caagtctcag cgttctttga gggttctgct tttcctgctc ggctatctct tttactgcgt
     1561 taacttcccg attaatggcg cgcagatgtt gcacaatgcg gtgacctgct gtggtcagtt
     1621 gtatagcttt acggcgatcg cgtaacagta tattcacacc aagttcttgt tcgagggcag
     1681 caattgcctg acttaccgcc gactgcgtca tgaacaatcg cttgctggct tcagtaaagc
     1741 caccgtaatc cagcacggcg agtaaagaat ttatttgggt aagagtcatt agagtttgct
     1801 aatcaataat attaaaacga ttaatttctc taatagcatg ccgccattta tacttctgtt
     1861 ctgcaaagta catcacaatt tgacgcaacc gcagtaacgg gagtgttgtc tgcgtgcgtc
     1921 atcctgacag gcggtagggt atgaaagaga agttatggac gaaggatttt tgggcaataa
     1981 ccatcatcag ctttattatt ttcttcgtct tttatgtttt actaacattg ttgccaattt
     2041 atatctctga ccgcttgcat gcctctcctg ataaagcagg tttgttggtg acattatttt
     2101 taattgcggc gattgttatt cgaccctttg ccggacaatg ggtgggtaaa tattcgaata
     2161 aaactattct ggtgctctct tctctggcct ttttggtggt cactgcgctg tatccttttt
     2221 gccactcaat agaatcactg ctttttatta gggtgcttca tggtattacc ttcggggtta
     2281 tcacaacggt aaagggaacg atttccgcgc ggctgatccc ggcctcccga cgtggggagg
     2341 gcatcagttt tttctctctg gcaatggggc tggcaatggt ggtcgggccg tggattggcc
     2401 tgaatatggc gcgctgggag gcctttaata tggctttctg gttatgtact ggcgttgcgg
     2461 cggtggggat tatcctgtcg ctgattatga ccgtgccgcc ggttatcagc catgccgatg
     2521 gttcaaagcc aaagatgggc ttcgccgcca tgttcgatcg cgcggcattg ccgtttgcca
     2581 tggttacatt ctttatgacc ttttcgtatg ccggggtttc tgcctttctg gcgctttacg
     2641 cccgcgaact taatctcatg tcggcggcca gtaatttcct gctctgctac gccatcttcc
     2701 tgatgatctg ccgtaccttc accggcaatg tttgcgacaa aaaaggcccg aaatatgtgg
     2761 tttacccctg cctgctgttc tttacggttg ggctggtggt tctcggctac acccagggca
     2821 gcgtaatgat ggtcgtttct ggcgcgttga ttggtatcgg gtatggttcc gtgacgccag
     2881 tttttcagac gcagattatc agttcagtgg aaccgcataa aatcggtgtc gcaaactccc
     2941 tcttcttcaa tgcgatggat gcaggcctgg cgctgggagc ctgtgtgatg gggatgatgg
     3001 ttgcacatac tggctaccga atgatttatc tgctgggcgc actattagtg gtagtggctg
     3061 gtggagtcta tgcgctgcaa atgaagggta aaagcggtgt cgcgctagta gtggcaaaag
     3121 aaattcatta atacgacacg tttcattaag atttttcgca ggtaaccacc tatagtcatt
     3181 gtcgttaata aaaaatgaca ggcgataggg tatggcagtt tcagcaaagc acgacgaatt
     3241 taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg
     3301 tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctatatgtaa agcgcatgac
     3361 gcatcatctg tttcattccg tacgttatcc tttcggccga ccaacgattg tccgtgaggt
     3421 tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga
     3481 agcggtgaaa attgggggtg aatggcgcgc gttgctggtg actgaagata tggcggggtt
     3541 catcagcatt gctgactggt atgcccagca tgcagtaacg ccctattctg acgaagtacg
     3601 gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg ttaatcgtca
     3661 gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg cagaagctgg
     3721 ttttcttgat ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga ttaatcatga
     3781 cttccgccag ctggaaaaat atctggagcc gatccccaaa gcggactggg agcaggtaaa
     3841 agcgtattac tacgccatgt aatcgttgta ggccagataa tacgcataag cgttgtatct
     3901 ggcactacaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc aacaacaggg
     3961 caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcca cctggcctgc
     4021 aacaaggcgc gtgcgacagg agccgcagta accttcgcgg cactggtact caaccgccac
     4081 attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca gcagttgtgt
     4141 gccagtgatg cgcagaataa cgcgggccat cagagctgga agttactcaa atcgtcggtg
     4201 tccacttccg agtcaatctg cccgaccaga taagaactca cctccacttc ctgtggagca
     4261 acctgcacgt tatcagacac cagccaggtg ttgatccacg ggatcgggtt ggagcgcgtc
     4321 tggaacggca gatccaaacc aaccgcctgc atacggatat tggtgatgta ctccacgtac
     4381 tggcagagaa tgtctttgtt cagaccaatc atcgaaccgt cacggaacag ataatccgcc
     4441 cagtctttct cctgttgtgc cgcctggaca aacaggtcat agcactcctg cttacattct
     4501 tcggcaattt ccgccatctc cggatcatcc gcgccgctgc gcagcagatt cagcatatgc
     4561 tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat tttggcgtta
     4621 ccttccatca gctcacgttc agcgaatgcg aaagagcaag caaagctgac gtagaaacga
     4681 atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt caactcgcgc
     4741 aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag caggtgccag
     4801 tagctggtca tttcgatcag ctcatcgtaa tagctggaga tcccttccgc acgtttctgg
     4861 atctgctcgt tggtgacgat atcgtcaaac acaacagacg gatcgttaac gatattacga
     4921 atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt ttcgacccag
     4981 gtttccagtt ccggaataga aataagcggc aatagcgcca cgttcgggct acgaccctga
     5041 atggagtcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgttca
     5101 ggcagcgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg acgccagaag
     5161 aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga
     5221 gccacgttga ccggctgacc aaagaacatc ggttctttga gctgatcatt tttcgtctgt
     5281 gaaaaggtgg tatatgccat gagtgtgtcc tgttgggagt ttaatgccgg ataaggcatt
     5341 ttacgccgca tcctgtgcct gatgcgacgc tggcgcgtct tatcaggcct acaaatccga
     5401 gccgtaggcc ggataaggcg ttcacgctgc atccggcacc cggagcctga tgcgacgctg
     5461 gcgcgtctta tcaggcctac aaaccgagcc gtaggccgga taaggcgttt acgccgcatc
     5521 cggcatctca atatcagatc ttacatgcgc cgctttcgca gccatcgtcc tggattgacg
     5581 gcaccagatc gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa tacagcgttt
     5641 tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa ttgctgcatc ggcacttttc
     5701 ctgacgggaa gcgtgacgga tcgtagttgg tgttagcaga gatcgactga tcgataaatt
     5761 tctgcatgat acccaccagt tgcaggtagc catcgttacc cggcatttcc cacagcagct
     5821 cataggcgtc gtgcagatgc tcgtagtccg gcactacctg gcgcagaata ccgtctttcg
     5881 acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg ttagagatct
     5941 gtgaagaagt ctcggacggc atcagggcag aaagcgtgga gttacgcaga ccgtgcgttt
     6001 tgatcgattc acgcagtgct tcccagtcgt aatgcagtgg ttcattagcg atggcatcca
     6061 gatctttctt ataggtatcg atcggcagga tccctttcgc gtaagtggtt tcgttaaacc
     6121 acgggcacgc gccttgctct ttcgccagct cattagacgc tttcagcagg taatactgaa
     6181 tcgcttcgaa ggttttatgc gtcaggttgt tggcgctgcc gtcggagtag cgtttaccat
     6241 gcttcgccag gtagtaagcg aagttgatca caccaatacc cagcgtacga cgacccatcg
     6301 ctccacgttt ggcggccggg atcgggtaat cctgataatc cagcagcgcg tcaagtgcac
     6361 gaaccgccag aattgccagc tcttccagtt catccaggct attaattgcg cccaggttga
     6421 aagcagacag cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg ttcagcggtt
     6481 tggtcggcag ggctatctcc aggcacaggt tagactgacg cactggcgcg atggccggat
     6541 caaacgggct atgggtattg cagtggtcaa cgttctgaat atagatacga ccggtagatg
     6601 cacgttcctg catcatcagc gagaacagct caacggcttt cacacgctgc ttgcggatgc
     6661 tgtcgtcttt ctcatatttg gtatacaggc gttcaaactc ttcctgatcg gcgaagaatg
     6721 cgtcgtacag ccccggtacg tcggacgggc tgaacagggt gatatcttca cctttcagca
     6781 ggcgggtata catcagtttg ttgatctgta ccccgtagtc catatggcgc acgcggttgc
     6841 cttccacacc acggttgttt ttcaacacca gcaggctttc cacttccaga tgccacatcg
     6901 ggtagaacag cgttgccgca cctccgcgca cgccgccctg agagcaggat ttcaccgcag
     6961 tctggaaatg tttgtagaac ggaatgcagc cggtatggaa cgcttcgcca ccgcgaatcg
     7021 ggctacccag cgcacgaata cgcccggcgt tgatgccgat cccggcacgc tgggaaacat
     7081 atttcacaat cgcgctggag gtggcgttga tggaatccag gctgtcgccg cactcgatca
     7141 gtacgcagga gctgaactga cgagtcgggg tacgcacgcc ggacatgatt ggcgtcggca
     7201 gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt cacgtattgc aggcgcgttt
     7261 cacgcgggta gttcgagaac aggcacgcag caaccagaat ataaaggaac tgggcgcttt
     7321 cgtagatttc gccggtcaca cggttctgta ccagatattt gccttccagc tgcttaacgg
     7381 cagcataaga gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc tgcttgaact
     7441 cttcttccgt gtagtcttcc agcagatgat tatcgtattt gcccatctcg accattttca
     7501 ccacatggtc atacagcgca ggtggctcaa actggccgta ggctttttta cgcaggtgga
     7561 agatcgccag gcgcgcggcg agatactgat aatccggcgc atcacgggag atcaggtctg
     7621 cggcagcctt gataatggtt tcgtggatgt cagaggtctt gataccgtca taaaactgaa
     7681 tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg cagtccttct gccgcccaat
     7741 ccagaacgcg atggattttg tcgagattga tgcgctctgt gctaccgtcg cgctttgtca
     7801 ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg aattctttcc gcaatagttt
     7861 catgcagact acctgtagta cgggaagctg cttagaacac tatatatagg gggtatgttt
     7921 gggattcact gcaagatagt gtgaaaatgg ccctcttgca agtgcataac tttgtggata
     7981 actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca gacaaggtgt ccgggaaagt
     8041 caatgggaag agaaaaattt gttaaaaata actgttcggc gatttcttga acggcataat
     8101 aggttagata aattgatata gatggtttat taatcttttt gctaatgatt gcaaaaattg
     8161 atctaagcac gagtcatcac ataatctgat gtatcagttg tggtcaatat ataagcgaat
     8221 gtaagcgatt gttgatgtcg aaatggttgt tttctattcg gttattgtta aatgcgaagc
     8281 cattttgtta atttacctta tattataaga aaattattca ttactttaat ttagtttaaa
     8341 tataactggt atcccttaag gttattaact tgaattatga cgcactgata ctattcatta
     8401 aataataaca aaatagctat tgcatcgggt tggacaattt acaaaagaaa gataacccat
     8461 attgtataat gcgtttaaat atgccgtctt atataaaatg atggtcagat taatatgcgg
     8521 attatctttc tacgcaagga gtatttatct ctactcccgt caatgattgc atctcttttc
     8581 tctgctaacg gtgtcgcggc ggccattgat ttatgccagg gatatgatat caaagcgagt
     8641 tgtcacgcca gcaggcaaag cctttcaggc attacgcagg tctggagtat cgccgatggg
     8701 caatggctgg ttttttcgga tacgaccaat aatgccagcg gtggggccgt atttttgcaa
     8761 caaggagcgg aatttacatt atcaccagaa aatgaaactg gaatgactct gtttgccaat
     8821 aacaccgttt caggagaata taataacggc ggggcaatat ttgctaaaga aaactcaacg
     8881 ctgaatctta cggatgttat tttttctggt aacgtcgcag gcggctatgg tggcgcaatc
     8941 tattcttctg gtactaacga taccggtgcc atcgagttac gtgtcactaa cgccgtgttt
     9001 cgcaataaca tcgctaatga cggcaaaggt ggtgcaattt ataccatcaa taatgatatc
     9061 tatttaagtg atgatgtttt taacaataac caggcatata catcaacaag ttacagtgat
     9121 ggcgatggcg gcgcaatcga tgtcacagat aataatagcg acagcaagca tccttcaggt
     9181 tatacgataa taaataacac tgcctttaca aataacactg ccgaaggtta tggcggggcg
     9241 atatatacca atagcgcgac ggctccctat cttattgata tttctgttga tgacagctac
     9301 agccagaacg gaggcgtgtt agtcgatgag aacaatagcg cagcaggcta tggagatggt
     9361 ccttcctctg cggcgggtgg ctttatgtat ctcggcttaa gtgaagttac atttgatatt
     9421 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
     9481 gctggtaccg ggttaatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
     9541 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
     9601 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
     9661 acgataggga gtattgataa gtaccagaat caggcagagc taaatgttgg ctccacccaa
     9721 caaacctttg agcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
     9781 agcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
     9841 accattgcgc aaaacggcag ctatgtgctg gcgggggcgc agtcgatggc gctaaccggc
     9901 gatatagtgg tggatgctgg tgcggtgctt tcgctggaag gcgacgcggc agatcttgcc
     9961 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
    10021 ttctccacct ggcagagcgg tacatcatac aaagatggcc ttgaagtcag tggcagcagc
    10081 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gcggaaacga tatgcatatc
    10141 ggcggcgacg ggaaagatgg cgtctacgtg gtgatcgatg cgggtgacgg gcaggtcagc
    10201 ctggcaaatg acaatcaata cctcggcaca acgcaaatcg cttccggtac gctgatggtg
    10261 agcgacaact cgcagcttgg agatacccat tataaccgcc aggttatctt taccgataag
    10321 ccacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac aacgactgag
    10381 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
    10441 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
    10501 ttgacgaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggcg
    10561 gtacgtgtcg aagaaggcac cctgaaaggt gatgttgctg atatccttcc ttatgcttca
    10621 tcactgtggg ttggtgacgg ggcaacgttc gagactggcg cggatcagga tattcagtct
    10681 atcgatgcta cttccagcgg cactatcgac atcagcgatg gtacggtttt gcgcctgacc
    10741 gggcaggata cttccgtcgc ccttgatgcc tcactgttta acggcgatgg tacgctggtg
    10801 aatgccaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
    10861 ctgacttatc tttccgacgt aacagttaat ggcaatctga ccaatacgac cggtgcggtc
    10921 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta caccggcggc
    10981 ggtacgttac tgttcgacag cgaattaaac ggcgatgact cagcaagcga ccagttggtg
    11041 ttgaacggta atactgctgg caacacaact gtggtggtta actccattac agggattggt
    11101 gagccgacat cgacaggcat tgaagtggtt gatttcgcag ctgatcccac gcagtttcaa
    11161 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
    11221 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
    11281 ccacctgatc cagacccgac tcccgatcct gatcccacgc cggatcctga tccaacaccc
    11341 gacccggaac ctacgcctgc ttaccagccg gtgttgaatg ccaaagttgg cggttatctc
    11401 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgcaggtggc
    11461 gatggtcaga cgctgaattt acgtgttatc ggcggagatt atcattacac agcggcaggg
    11521 caactggctc agcatgaaga cacttctacg gtgcagctta gcggtgacct gtttaggggg
    11581 cgctggggca cggatggcga gtggatgctt gggattgttg gtggctacag cgataaccag
    11641 ggcgacagcc gctcgagtat gaccggaact cgcgccgata accagaacca cggttatgcc
    11701 gttgggctaa cctcaagctg gtttcagcac ggtaatcaga agcagggggc ctggctggat
    11761 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcgtggat
    11821 cattaccatt cgtcggggat tatcgcctcg ctggaagcgg ggtatcagtg gttaccgggg
    11881 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
    11941 tttaccgccg ctaaccgtgc gcgcgtgtca caatcgcagg gtgatgatat tcagacgcgg
    12001 ctgggtttac acagcgaatg gcgtaccgct gttcatgtca taccaacatt agatctgaat
    12061 tattatcacg atccccattc gacggaaatt gaagaggatg gcagcactat cagtgacgat
    12121 gcggtgaagc aacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
    12181 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
    12241 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcactattg ccggatgcgg
    12301 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gcggcaagcg
    12361 tcgcatcagg cattggcgcg gttgccggat gaatccaaac aacaacctca cttattctgc
    12421 gtgtgcagca tatagttcac atccacgccg gggccgagtt taaaagtatt agtgatcggg
    12481 ttgtaatgta gcccggttat atgtcgctct ttaaggcttg tctgatctac ccagccaagc
    12541 agctctgccg gtttaataaa cttcttcaca tcatgcgtgc ctttgggcac catgcgcaaa
    12601 atatattccg cgccaaccac cgccatcagc catgacttgc cgttgcggtt aagcgtcgag
    12661 aaaaagacat cgccgcccgg tttcaccagt tgcgcacagg ctctgaccac tgactgcgga
    12721 tcggggacgt gctccagcat ctccatgcag gtcaccacgt catactgccc ggcatgtttt
    12781 gccgcgtgct cttccacagt ttcttgcacg taatccactt gaatgccgct ttccagcgcg
    12841 tgcagttttg ccacctgcaa tggctcaaag cccatatcca gaccggtcac cgtcgcacct
    12901 tcgcgcgcca tactctcggc cagaatgccg ccgccacaac cgacatcgag cacctttttg
    12961 ccaaataagc cgccagcacg ctcggcaata tagcccagac gcagcgggtt aatgcggtgc
    13021 agcggtttga actcaccttc cagatcccac cagcgggagg cgacggcttc aaatttagcg
    13081 atctcttcgt ggtctacgtt atggtttacc ggcgattttt cggcattcat tggcacttct
    13141 actccgtaat tggcaagaca aacgagtata tcaggcattg gatgtgaata aagcgtatag
    13201 gtttacctca atctgcgcgg ctgtgttata atttgcgacc tttgaatccg ggatacagta
    13261 gagggatagc ggttagatga gcgaccttgc gagagaaatt acaccggtca acattgagga
    13321 agagctgaag agctcctatc tggattatgc gatgtcggtc attgttggcc gtgcgctgcc
    13381 agatgtccga gatggcctga agccggtaca ccgtcgcgta ctttacgcca tgaacgtact
    13441 aggcaatgac tggaacaaag cctataaaaa atctgcccgt gtcgttggtg acgtaatcgg
    13501 taaataccat ccccatggtg actcggcggt ttatgacacg atcgtccgta tggcgcagcc
    13561 attctcgctg cgttacatgc tggtagacgg tcagggtaac ttcggttcca tcgacggcga
    13621 ctctgcggcg gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc atgaactgat
    13681 ggccgatctc gaaaaagaga cggtcgattt cgttgataac tatgacggca cggaaaaaat
    13741 tcccgacgtc atgccaacca aaattcctaa cctgctggtg aacggttctt ccggtatcgc
    13801 cgtaggtatg gcaaccaaca tcccgccgca caacctgacg gaagtcatca acggttgtct
    13861 ggcgtatatc gatgatgaag acatcagcat tgaagggctg atggaacaca tcccggggcc
    13921 ggacttcccg acggcggcaa tcattaacgg tcgtcgcggt attgaagaag cttaccgtac
    13981 cggtcgcggc aaggtatata tccgcgcccg tgctgaagtg gaagttgacg ccaaaaccgg
    14041 acgtgaaacc attatcgtcc acgaaattcc gtatcaggta aacaaagcgc gcctgatcga
    14101 gaagattgcg gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc tgcgtgacga
    14161 gtctgacaaa gacggtatgc gcatcgtgat tgaagtgaaa cgcgacgcgg tcggtgaagt
    14221 tgtgcttaac aacctctact cccagaccca gttgcaggtt tctttcggta taaacatggt
    14281 ggcattgcac catggtcagc cgaagatcat gaacctgaaa gacatcatcg cggcgtttgt
    14341 tcgtcaccgc cgtgaagtgg tgacccgtcg tactattttc gaactgcgta aagctcgcga
    14401 tcgtgctcat atccttgaag cattagccgt ggcgctggcg aacatcgacc cgatcatcga
    14461 actgatccgt catgcgccga cgcctgcaga agcgaaaact gcgctggttg ctaatccgtg
    14521 gcagctgggc aacgttgccg cgatgctcga acgtgctggc gacgatgctg cgcgtccgga
    14581 atggctggag ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg aacagcaagc
    14641 tcaggcgatt ctggatctgc gtttgcagaa actgaccggc cttgagcacg aaaaactgct
    14701 cgacgaatac aaagagctgc tggatcagat cgcggaactg ttgcgtattc ttggtagcgc
    14761 cgatcgtctg atggaagtga tccgtgaaga gctggagctg gttcgtgaac agttcggtga
    14821 caaacgtcgt actgaaatca ccgccaacag cgcagacatc aacctggaag atctgatcac
    14881 ccaggaagat gtggtcgtga cgctctctca ccagggctac gttaaatatc agccgctttc
    14941 tgaatacgaa gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta ttaaagaaga
    15001 agactttatc gaccgactgc tggtggcgaa cactcacgat catatcctgt gcttctccag
    15061 ccgtggtcgc gtctattcga tgaaggttta tcagttgccg gaagccactc gtggcgcgcg
    15121 cggtcgtccg atcgtcaacc tgctgccgct ggagcaggac gaacgtatca ccgcgatcct
    15181 gccggtgacc gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta acggtactgt
    15241 gaagaaaacc gtcctcaccg aattcaaccg tctgcgtacc gccggtaaag tggcgatcaa
    15301 actggttgac ggcgatgagc tgatcggcgt tgatctgacc agtggcgaag atgaagtaat
    15361 gctgttctcc gccgaaggta aagtggtgcg ctttaaagag tcttctgtcc gtgcgatggg
    15421 ctgcaacacc accggtgttc gcggtattcg tttaggcgaa ggtgataaag tcgtctctct
    15481 gatcgtgcct cgtggcgatg gcgcaatcct caccgcaacg cagaacggtt atggtaaacg
    15541 taccgcagtg gcggaatacc cgactaagtc gcgtgcgacg aaaggggtta tctccatcaa
    15601 ggttaccgaa cgtaacggtt tagttgttgg cgcggtgcag gtagatgact gcgaccagat
    15661 catgatgatc accgatgccg gtacgctggt acgtactcgc gtttcggaaa tcagcatcgt
    15721 gggccgtaac acccagggcg taatcctcat ccgtactgcg gaagatgaaa acgtggtggg
    15781 tctgcaacgc gtggctgaac cggttgacga ggaagatctg gacaccatcg atggcagtgc
    15841 tgcggaagga gacgacgaaa tcgctccgga agtggacgtt gacgacgagc cagaagaaga
    15901 ataattttac ttcttcatgc caaaagggag ctatctccct tgtttgaatt gaaaagtcca
    15961 ggctgcaaag tctgggcttt tgtcgtatta gggcacggta aagtttggct gtgcccgtaa
    16021 aaaatggctg gctatacaca aggaatgtgg caatgagtgg tgaaaaaaag gcgaaaggct
    16081 ggcggttcta tggtcttgta ggttttggcg caatagtact gctttccgct ggcgtctggg
    16141 cgttgcaata tgctggcagt gggccagaaa aaacgttgtc gccgctggtg gtgcacaaca
    16201 atctgcaaat cgatctcaat gagccggacc tctttctcga cagcgactct ctgagccagc
    16261 ttcccaaaga tctcctcacc attccgtttc tccacgatgt tctgagtgaa gatttcgttt
    16321 tctattatca gaatcatgcc gatcgtctgg gcattgaagg cagcattcgt cgcattgtct
    16381 atgaacacga tctcacgctg aaagataaac tcttttcgtc actcttagat cagcccgcac
    16441 aggcggcgct atggcacgat aaacaaggcc atctttcaca ttatatggtg cttatccagc
    16501 gtagtggctt aagcaagctg ctggagccat tgctgtttgc cgctaccagc gacagccagt
    16561 taagtaaaac ggaaatcagt agcatcaagc taaataatga aactattcct gtttatcagt
    16621 tgcgctataa cggcaataac gccctgatgt tcgcgacata tcaggacaag atgctggtgt
    16681 tttccagcac ggatatgctg tttaaagatg aagagcagga taccgaagct acggcgatcg
    16741 caagtgattt gttgagcggt aaaaaacgct ggcaagtcag ctttggcctg gaagagcgta
    16801 ctgccggaaa aacaccagta cgccagcgca tcgtggtcag cgccaggttg ctgggttttg
    16861 gctaccagcg attaatgcct tcttttgccg gagtgcgttt cgaaatgagc aacgacggct
    16921 ggcatagctt tctggcgtta aatgatgaat ccgccagcgt agataacagt ttcgatttta
    16981 cgccggtctg gaacagtatg cctgccgggg ccagcttctg tgtggcggtg ccgtattcac
    17041 acggtattgc cgaagagatg ctttcgcaca tcagccagga aaacgacaag ctgaatgggg
    17101 cgttagacgg tgccgcgggg ctgtgctggt atgaagactc aaaattgcaa accccgctgt
    17161 ttgtcggtca gtttgatggc actgccgaac aggcgcaatt gccagggaaa ctgtttacgc
    17221 aaaatattgg tgcgcacgaa agcaaagcgc cagaaggtgt tttgccggta agccagactc
    17281 agcagggcga agcgcaaatc tggcgtcgcg aagtgagttc ccgatacggt cagtatccga
    17341 aagcgcaggc ggcgcaaccc gatcaattaa tgtcggatta ttttttccgc gtgtcgctgg
    17401 cgatgcaaaa caaaacgctg cttttctccc tcgatgacac gctggttaat aacgccctgc
    17461 aaacactgaa taaaacccgc ccggcaatgg tggatgtaat acccactgat ggcatcgttc
    17521 cgctctatat caatccacaa ggcgtggcga aactgctgcg taacgaaacg ttgaccagtc
    17581 tgccgaagaa tctcgaaccg gttttttata acgccgcaca aactttatta atgccgaagc
    17641 tggacgcttt atctcaacaa ccgcgttatg tcatgaagct ggcccagatg gaacccggtg
    17701 ccgcctggca gtggctaccc ataacctggc agccgctatg aggcacgggc tgctggcgct
    17761 gatttgctgg ctgtgttgtg ttgttgccca tagcgaaatg ctgaatgtcg aacaatccgg
    17821 gctgtttcgc gcctggtttg tacgcattgc acaagaacag ctccgccagg ggccaagtcc
    17881 acgctggtat cagcaggatt gtgcgggcct ggtgcgattt gcggcgaacg agacgctgaa
    17941 agttcacgac agtaaatggc taaaaagtaa cggtttatct agccagtatt tgccgccaga
    18001 gatgacgcta acacctgaac agcgtcaact ggcgcaaaac tggaatcagg ggaacgggaa
    18061 aaccggcccc tacgtgaccg cgattaattt gattcagtac aacagccagt ttattggcca
    18121 ggacataaac caggcgctgc ctggcgatat gatttttttc gatcagggca atgcccagca
    18181 cttaatggtc tggatggggc gttacgtcat ctaccacacc ggaagcgcca cgaaaactga
    18241 caacggaatg cgcgcagtca gtctgcaaca acttatgaca tggaaggaca cccgatggat
    18301 acccaacgat tccaatccca atttcattgg catttatcgt ttaaattttc tggcgcgata
    18361 gcagcgtgct tgtccctgtc tcttgtggga acagggcttg ctaatgctga tgattcgctt
    18421 ccttccagta actatgcgcc gcccgcaggg ggaacattct ttttgcttgc tgacagcagt
    18481 tttagcagca gtgaagaggc gaaagtgcga ctggaagcgc cggggcgtga ttatcggcgc
    18541 tatcagatgg aagagtacgg cggcgtggac gttcgcctgt atcgtattcc tgacccgatg
    18601 gcatttttgc gccagcagaa aaacctgcat cgcattgtgg tgcaaccgca atatttgggc
    18661 gacgggctga acaatacgct gacctggctg tgggataact ggtacggcaa atctcgccgc
    18721 gtgatgcagc gtactttctc ttctcagtca cggcagaatg tgactcaggc attacccgaa
    18781 ttacatctcg gcaatgccat tattaaacct tcccgttatg tacagaacaa ccagttttcc
    18841 ccgctgaaaa aatatccctt ggtggaacag ttccgttatc cactatggca ggctaaaccg
    18901 gtcgagccgc agcaaggggt aaaactggac ggcgcatcca gcaatttcat ctcgccgcag
    18961 ccgggtaaca tttatattcc tctcggccaa caagagccgg gactgtacct cgtcgaggcg
    19021 atggttggtg ggtatcgggc gacgacggtg gtgtttgttt ccgataccgt ggcgcttagc
    19081 aaagtgtcag gcaaagagct tctggtgtgg accgcgggta aaaaacaggg tgaagcgaag
    19141 cccggctcag agatcttgtg gactgacggt cttggcgtga tgacccgcgg tgtgaccgat
    19201 gacagcggta ccttgcagtt acaacatata tcgccagaac gttcatacat tctgggtaag
    19261 gatgctgaag gcggcgtttt tgtctccgag aacttcttct acgaaagcga aatctacaac
    19321 acccgcttgt atatttttac cgatcgcccg ctatatcgcg caggcgatcg tgtcgatgtt
    19381 aaagtgatgg gccgcgagtt ccacgatccg ttgcattcat cccccatcgt cagcgccccg
    19441 gcgaagcttt cggtgctgga cgctaacggc agtctgttgc aaaccgtcga tgtcacgctg
    19501 gatgcgcgca atggcgggca gggaagtttc cgcctgccag aaaatgccgt tgccggaggt
    19561 tatgagttac gtcttgctta ccgcaatcag gtctatagca gtagttttcg cgtagccaac
    19621 tatatcaagc cacatttcga gattggttta gctctcgaca aaaaagagtt caaaactggc
    19681 gaagcggtca gcggcaaact gcaacttctc tacccggatg gcgagccggt aaaaaatgcc
    19741 cgcgtgcagt taagtttgcg cgctcagcaa ttatcaatgg tcggtaacga tttgcgttat
    19801 gccggacgtt tccccgtgtc gctggaaggc agtgaaacgg tgtccgacgc cagcggtcat
    19861 gtgacgttaa atctccccgc cgccgataaa ccgagccgct atttgttaac cgtctccgcc
    19921 agtgacggcg cggcgtatcg cgtcaccacc accaaagaga tcctcattga acgcggcctg
    19981 gcgcattact ctttaagtac cgccgcacaa tacagtaata gcggcgagtc ggttgtgttc
    20041 cgttatgccg cgctggaatc ttcaaaacag gttcctgtta cgtatgaatg gttgcgtctc
    20101 gaagaccgca cgagccatag cggagagcta ccgtctggcg gcaaatcctt taccgtcaat
    20161 ttcgataaac ctggcaacta caatctgacg ttacgcgata aagacggctt aattctcgct
    20221 gggttaagtc atgccgtcag cggtaagggc agcatgtcgc atactggtac ggtagatatc
    20281 gtggcagata aaacgctgta ccagccaggc gaaaccgcga agatgttgat tacctttcca
    20341 gaaccaattg atgaagcctt gctgacgctg gaacgcgatc gcgtggaaca gcagtcgctg
    20401 ctttcgcacc cggcaaactg gataacgcta caacgcttaa acgataccca gtatgaagcc
    20461 cgcgttccag tgaacagtac ctttgcgccc aacatcactt tttcggtgct gtacacccgt
    20521 aacgggcagt acagttttca gaacgcaggg atcaaagtcg ccgttcccca gttagatatt
    20581 cgggtgaaaa cggataaaac ccactaccag ccgggcgaac tggtcaacgt cgaattaacc
    20641 tcgttgctga aaggtaaacc ggtttctgcg cagctaacgg ttggtgtggt cgatgaaatg
    20701 atctacgcgc tacaaccgga aatcgcgccg aatatcggca aatttttcta tcccctgggg
    20761 cgaaacaatg tgcgcaccag ttccagcctg tcatttatca gctatgacca ggcgctctcc
    20821 agcgagcctg ttgcgcctgg cgcgactaac cggagcgagc ggcgagtaaa aatgctggaa
    20881 cgtccacggc gtgaagaggt ggataccgct gcatggatgc cgtcgctaac aacggataaa
    20941 caaggcaaag cgtactttac gttcctgatg ccggattcgt taacgcgctg gcgcattacc
    21001 gcgcgtggga tgaacggcga cgggctggtc gggcaggggc gtgcttatct gcgttcggaa
    21061 aaaaatctct acatgaagtg gagtatgcca acggtgtatc gcgtgggcga caaaccgtcg
    21121 gcaggactgt ttatcttcag tcagcaggat aacgaaccgg tggcgctggt gactaaattt
    21181 gcaggcgctg agatgcgcca gacgctgacg ctgcacaaag gggcgaatta tatttcgctg
    21241 gcgcagaaca ttcagcaatc tggcttgtta agcgcagaac tgcaacaaaa tgggcaagtg
    21301 caggacagca ttagcacaaa actgtctttt gtggataaca gctggcccgt tgaacagcag
    21361 aaaaatgtca tgctcggtgg tggcgataac gcgctgatgt tgcccgagca ggcgagcaat
    21421 atccggctac aaagtagtga aacgccgcag gagattttcc gcaacaatct tgatgcgtta
    21481 gtcgatgaac cgtggggtgg ggtgatcaac accggtagcc gtctgatccc gctcagtctc
    21541 gcctggcgtt cgcttgccga tcatcaaagt gccgccgcta acgacattcg tcagatgatt
    21601 caggataacc gtctgcggct gatgcaactg gcggggcccg gagcgcgctt tacctggtgg
    21661 ggtgaagatg gcaatggtga cgccttcctt acggcatggg catggtacgc cgactggcag
    21721 gccagccagg cgctcggcgt aacgcaacaa ccggaatact ggcagcatat gctcgacagt
    21781 tatgccgagc aggcagataa catgccgtta ttgcatcggg cgctggtgct ggcgtgggca
    21841 caggagatga atctgccgtg caaaacgttg ttgaaagggt tggatgaagc tatcgcccgg
    21901 cgcggaacta aaactgaaga tttctctgag gaagcccccc gcgatatcaa tgacagcctg
    21961 atcctcgata caccggaatc tccactggca gatgcggtgg caaacgtctt aaccatgacg
    22021 ttgctgaaaa aagcgcagtt gaagtccacg gtgatgccac aggttcagca atatgcgtgg
    22081 gataaagcgg caaacagcaa tcagccgctg acgcacacgg ttgtgctgct caatagcggg
    22141 ggcgacgcta cccaggcggc tgctatttta agtggtttga ccgctgagca atctactatt
    22201 gagcgcgcgc tggccatgaa ctggctggcg aaatatatgg cgacaatgcc tccagttgtt
    22261 ttgcctgcgc ctgcgggcgc atgggccaaa cataagttaa ctggaggggg cgaatactgg
    22321 cgttgggttg gtcagggcgt gccggacatt ctctcttttg gtgacgaatt atcgccgcaa
    22381 aatgtgcagg tccgctggcg tgaaccggca aaaacggctc aacaaagtaa cattccggtg
    22441 accgttgaac gccagttgta tcggcttatc cccggtgaag aagagatgag ctttactctg
    22501 cagccggtga ccagcaatga gattgacagc gatgcgctgt atctcgatga aattacgctt
    22561 accagcgagc aggatgcagt tctgcgctat ggtcaggtgg aagtaccgct cccgccggga
    22621 gccgacgttg agcgcacaac atggggcatt tcagtcaata aacccaacgc cgcgaaacag
    22681 caggggcaat tgctggaaaa agcgcgtaat gaaatgggcg aactggccta tatggtgccg
    22741 gtgaaagaac tgacgggaac ggtcactttc cgccatttgc tgcgcttctc gcaaaaaggg
    22801 caattcgttc tgcctcctgc tcgttatgtg cgttcctatg cacctgcaca gcaaagtgtt
    22861 gcggcaggga gtgaatggac cgggatgcag gtgaaataag tgaactggcg cagaatcgtc
    22921 tggctattgg cgttggttac tcagccaacg cttgcggagg agacgccgtt acaactggcg
    22981 ctgcgcggtg cgcagcacga tcaactgtat cagctgtcgt catcaggcgt gaccaaagtc
    23041 tcagcattac ctgactcgct caccacgccg ttagggagtt tgtggaaact gtatgtttac
    23101 gcctggctgg aggataccca tcaaccggag cagccctacc agtgtcgcgg aaactccccg
    23161 gaagaggttt attgttgtca ggctggggag agcataacgc gcgataccgc gctggtacgt
    23221 tcctgtgggc tctattttgc gccgcagcgg ttgcatattg gcgcagatgt gtgggggcaa
    23281 tactggcaac agcgtcaggc acctgcgtgg ttagcgtctc ttactacgct gaagccagaa
    23341 acctcggtaa cggttaaatc gctgcttgat tcattagcga cgttacctgc gcaaaacaaa
    23401 gctcaggaag tcttactgga tgtggtgctg gacgaggcga aaatcggtgt cgcatccatg
    23461 ctcggtagtc gggtacgggt gaaaacctgg agctggtttg ccgatgataa acaggaaatc
    23521 cgtcagggtg gttttgctgg ctggttaacc gatggtacac cgctgtgggt tactggcagt
    23581 ggcaccagca aaacggtact gacccgttat gcgacggtgc ttaatcgggt tttgccagtg
    23641 ccgacacaag ttgccagtgg gcaatgtgta gaggtggagc tatttgcgcg ctatccgctg
    23701 aagaaaatca cagcggaaaa gagtacgacg gcagtaaaac ccggcgtact taatgggcgc
    23761 tatcgtgtga cctttaccaa tggtaaccac attacgtttg tcagccacgg tgagacgact
    23821 ttactgaacg aaaagggcaa actgaaattg cagtcgcatc tcgatcgtga agagtacgtt
    23881 gccagagttc tggatcgcga ggcgaaaagt acgccacctg aggcagcgaa agccatgacc
    23941 gtggcgatac gcactttttt acagcaaaac gccaatcggg aaggcgattg cctgaccatc
    24001 cccgacagca gcgccacgca gcgtgtttct gcttcgccag ctaccacggg ggcgcgaacg
    24061 atgaccgcct ggacgcagga tctcatctat gcgggcgatc cggttcatta tcacggcagt
    24121 cgcgctaccg aagggacgct ttcctggcgg caagccatgg cccaggcggg gcagggagat
    24181 cgttacgacc agatactcgc gtttgcttat cccgacaaca gccttagccg ctggggagcg
    24241 ccacgctcaa cctgtcagtt attacccaaa gcgaaagcct ggctggcgaa aaaaatgccg
    24301 cagtggcggc gtatattaca aggcgagacg gggtacaacg aaccagacgt gtttgcggtc
    24361 tgtcgtttag tctcaggttt cccctatacc gatcggcagc agaaacggct gtttattcgt
    24421 aacttcttta cgcttcagga tcggctggat ttaacccatg agtatctgca cctggccttt
    24481 gatggttatc ccaccgggct tgatgagaac tatatcgaaa cgctgacccg acaattatta
    24541 atggactaat gctatgcgaa agatttttct tccgttgtta ctggtggcgc tttcgcctgt
    24601 cgctcacagt gaaggtgtgc aggaagtcga gattgacgca ccgctctcag gctggcatcc
    24661 ggcagagggc gaagatgcca gtttttcaca atcgatcaat tacccggcgt cgtccgtcaa
    24721 catggcagat gatcaaaata tctcagcgca gatccacggc aaaataaaga attatgctgc
    24781 ggcgggtaaa gttcagcagg gccggctggt ggtcaacggt gccagtatgc cgcagagaat
    24841 tgaatccgat ggttcatttg cacgccctta tattttcact gaaggcagca acagcgtgca
    24901 ggtcatcagc ccggatgggc aaagccgaca aaaaatgcag ttttactcaa cgccgggtac
    24961 aggaacgatt cgtgcacgtt tacggctggt tctctcgtgg gatacggaca ataccgatct
    25021 cgatctccat gttgtcacgc ccgatggcga acacgcctgg tacggtaaca cggtgctgaa
    25081 aaacagtggt gcactggata tggatgtcac gacggggtac ggacccgaga ttttcgccat
    25141 gccagcgcca gttcacggac gctatcaggt gtatatcaac tactatggtg ggcgcagcga
    25201 aacggaatta accaccgccc aactgacgct gatcaccgat gaaggatcgg tcaatgagaa
    25261 acaggaaaca tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg tgaaaagttt
    25321 tgactggtga tccatccctt cacacaacct ggctctttct tttttcagat aagactgctg
    25381 ggcgatttgc cgacgccagg atataaccat taacaatcgt cggcagattg gtcatttctt
    25441 atttttcgta ctgagaactg tccatttact ccggctttcc tggttaaggc aatggtttct
    25501 aagcgatacg cattattgca atggtgacag aacattgcgg taacacgctt ttaccgctac
    25561 cttaaccaca ctccatcggt cacctgaggc ggagcttcgc ccctttgaaa taccttgctt
    25621 cttttcgtac aactctgaaa gcctcgcgct acatgttcag agcattggcg ttagtgctct
    25681 ggctgttgat tgctttttca tccgtttttt acatcgttaa tgcgttacat cagcgagaat
    25741 cggaaattcg tcaggaattt aatctgagtt ccgatcaggc tcagcgcttt attcaacgca
    25801 cctctgatgt gatgaaagag ctgaagtaca tcgctgaaaa tcgcttatcg gcagaaaacg
    25861 gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt gcctgcgttt gaaccgctgt
    25921 ttgccgactc tgattgttcc gcaatgagta acacctggcg aggttctctg gagtcattgg
    25981 cgtggtttat gcgctactgg cgcgataatt tttctgcggc ttacgatctc aaccgggtat
    26041 ttttaatcgg cagcgataac ctctgcatgg ccaatttcgg tctgcgtgat atgccagtgg
    26101 aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa taaatatcga aatgcaccac
    26161 aagatgatag cggcagtaac ctctactgga tcagcgaagg tccgcgccct ggcgtcgggt
    26221 atttttacgc gttgacgcca gtttatctgg cgaacaggtt gcaggcgctt ttgggtgtcg
    26281 agcagaccat ccggatggag aattttttct taccgggtac gttgccgatg ggggttacca
    26341 ttcttgatga aaatggtcat accctgattt cgcttaccgg accagaaagt aaaattaagg
    26401 gcgatcctcg ctggatgcag gaacgctcct ggtttggcta tacggaaggg ttccgggagc
    26461 tggtgctgaa gaaaaatctg ccaccctcat cgctaagcat cgtgtattcg gtgccggttg
    26521 ataaggtgct ggaacgcatt cgcatgttga tccttaacgc aattttgctg aatgtgcttg
    26581 ccggagctgc attgtttact ctcgcgcgga tgtacgagcg acgtattttc attccggcgg
    26641 aaagcgacgc cctgcgactg gaagaacatg agcagttcaa tcgcaagatt gtcgcctccg
    26701 cgccagtggg tatctgcatt ttgcgtaccg ctgatggcgt caatatttta agtaacgaac
    26761 tggcgcatac ctatctcaat atgcttacgc atgaggaccg ccaacgactg acacaaatta
    26821 tctgtgggca gcaagtcaat tttgttgatg tcctgaccag caacaatacc aatctgcaaa
    26881 tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc catttgtgtg ctggtggatg
    26941 tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat ggcacaagca gcggaacagg
    27001 cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca tgagctgcga acgccgctgt
    27061 atggcattat cggtaacctg gatctgttgc aaaccaaaga gttaccgaaa ggcgtcgatc
    27121 gtctggtgac ggcaatgaac aactcttcca gcctgttgtt gaaaattatc agcgatattc
    27181 tcgatttctc gaagattgaa tcggaacagt tgaagatcga accgcgtgag ttttcaccgc
    27241 gtgaagtgat gaaccacatc accgccaact atttaccgct ggtggtacgc aagcagttag
    27301 gcttgtactg ctttattgaa ccggatgtgc cagtggcttt aaatggcgac ccgatgcgtt
    27361 tacagcaggt catctccaac ctgttgagta acgccataaa attcaccgat accggctgta
    27421 tagttttgca tgtccgcgct gatggcgatt atctctctat ccgtgttcgc gataccggcg
    27481 tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc cttcttccag gtcggaacgg
    27541 gcgtacagcg aaacttccag gggaccggtc tgggtctggc gatttgtgaa aaactgatca
    27601 gcatgatgga cggcgatatc tcggtagatt cagaaccggg aatgggcagc cagtttaccg
    27661 tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa aggcgtggaa gggctgagtg
    27721 gtaaacgctg ctggctggcg gtccgcaatg cgtcgctctg tcagttcctg gaaaccagtt
    27781 tgcagcgcag cggcatcgtc gttacaacat acgaagggca ggaaccgact cccgaagatg
    27841 tgttaatcac tgacgaggta gtgagtaaaa aatggcaggg cagagcggta gtgaccttct
    27901 gtcgtcgcca tattggtatt ccgctggaga aagcgccagg ggagtgggta cacagtgtgg
    27961 ctgcaccgca tgagctaccg gcattgttgg cgcgtattta tttgatcgag atggagagcg
    28021 acgatcctgc taacgctctg ccgtcgacgg acaaagcggt cagcgataat gacgatatga
    28081 tgattctggt cgtggatgat catccgatta accggcgttt gctggcagat cagttgggat
    28141 cgttgggcta tcaatgtaaa accgcgaatg atggcgtcga tgcgcttaat gtacttagca
    28201 agaatcatat tgatatcgtg cttagcgacg tcaacatgcc caatatggat ggttaccgct
    28261 tgacgcaacg cattcgtcag ttgggactga cgttgccggt aatcggagta acagctaatg
    28321 cgttggctga agagaagcag cggtgtctgg agtccggtat ggacagctgt ctgtcgaagc
    28381 cagtaacgct ggatgtgata aaacagacgc tgacggtata tgccgagagg gtcaggaaat
    28441 cgcgggaatc gtaggccgga taaggcgttt acgccgcatc cggcattcag tgcaaatgcc
    28501 agatgcgacg ctgacgcgtc ttatctggcc tactttaatg taatctgttg aaataatggg
    28561 aatcgtaggc cggataaggc gtttacgccg catccggcat tcagtgcaaa tgccagataa
    28621 gacactaacg cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaaggt
    28681 cacagaagag agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc
    28741 agatttcttc tggctactga tggttttaat actgcggttc agctttttag cgatctcggt
    28801 caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt
    28861 gtcaccgtaa ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa
    28921 tttcttccct ttctgcagcg cggcgagggc tttcggcaga tcggtcggtg caccttgttt
    28981 cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt
    29041 catggtcaga acaataatcg acaggcttgg gaaatggcgc ttgatgtact taattaaggt
    29101 aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc
    29161 cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac
    29221 ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc
    29281 aataattacg ttcatattgt tcatgtactt gtacctatgt attaactatt tgattttaaa
    29341 aatatattat atgctttgtt tagtcgttgg ggcacctgtg ggacataagt acatagcttt
    29401 ctgtttagta cttctacctg ggactggtta ttgtctttca tccatgcacc gtaaacattg
    29461 taaaccatct gggcattcgc atgtcccatc tggttggcta tgaagtttgg atttgctccg
    29521 gcagttaaag cccagcaagc ataagtatgc cgagactgat acgcttttct atggcgaatt
    29581 tttgctcgtt ttaatgcaga ttcccatgtt ctgttgatgg atgttacggc ataatgaact
    29641 cctgagttac ctgtgcgatc taatatctga ggattgaaaa caaagctgca tgagtgagtg
    29701 cttgtccggc catactctcg cagctttact tctatctgat attgtttacc aagcctggtt
    29761 aatgtcgcct gattttttaa tacgtcaatt gctggctgta tgagttgtat taccctgttt
    29821 gttcctgcat ctgttttggg tagtgtaaat tcctttgtct gagtgtaatt ccttttgacg
    29881 gtcaaggtac ccgcttccag atcgatatcc tcccatgcca gcccacacag ttcgccatgc
    29941 cgcatcccgg tatatacagc cagcgaccag aaatttttag tttgctggtg gtggcacgca
    30001 tcaatcagtc tggcaaactc atcttttgtt agtggatcgg gaggtgtttt cgctcttttc
    30061 agtgatggaa gctcactgaa agggtttttg cctatatacc cattgtttgc cgcaaaacgg
    30121 aacatcccgg agataattgt catatagcta tttacagtag gtacactacg acctttaata
    30181 ggcttcctct gattttttcc tggcaactga tatcccgtta ataattcttt acgaaaaatg
    30241 agaacatctt cctgtgtaat tgatgatgcc agtttgttcc ctccaaggca gtcaaggcaa
    30301 tttcggatag ctgatttgta tctgaccatg gcatttgctg ttatttccat tttcttaagc
    30361 tcaagccatt tttccgaaag tgccttaatg gttatctctt tttttcccag accaaagtgt
    30421 ttcaggttag gggaattagg gaactgcgcg gcgtagtcga agctccccat tctgattgca
    30481 aaacaaacgg aagtacgaag ttcacctgcg atcttccggt ttttggcggt gtcaggaaca
    30541 ccgaggtttt ctctgacacg tttgccgtta tagtgaaacc atatgcggag tgatccgcca
    30601 tggttttcaa cgcctgtcgg gtatgatgcg ttactcatta aacctcccag acgtccagga
    30661 gcattaacag gttaaccgga acttgcattt ttggcacctg gttgtttctg gttttcgatc
    30721 cagcgcataa tttcctcaat gttgtataca cattcactga aaggcccggg ctctccttct
    30781 gctgcgtaat gacggtattc tttccccagt aaccaggatt tttttcttgc tctggcaatg
    30841 gttcctgatt tcagtccagt tgcagcaata agggttcttt ctgtgcacca tttgctgggc
    30901 gttatctgat agatgattgt ttccattcct gactcacact atcttcaggc cacggcagtg
    30961 gcaccacacg tcaaacattc gctttacaac ttcacggcag tagaagccgt gagcatctct
    31021 ggttaaatcg tatcggtacc cgtatctgag tcgtatccat atttcaaact cgcgattcac
    31081 gacttattct cctgctgtct ttatgccagt tcatccggtt gcgctggggc ccaggccttc
    31141 cgtggcctgt atttgcggcg ttttctggct tcaatgcgta tctttgtcac cctgactggc
    31201 tttggttcgt gccggaacac tttgtttctg atattacgcc gtgtattcat catccagata
    31261 aaacgctggg tatggtcgca tccgtcatca acggtaatta ctgtgcggac cagttcatcg
    31321 ttaatatcaa ccatttcacc cattcactga gccctcatac gcgaatttcg tggcttccat
    31381 cagttgcgtc catacccatt caagaaattg tgcgttttct cctgagcggt catattcatc
    31441 gacgctgcgg ggcttgatgg tcgccagtag tggcacaatc cggtacttca tctgttcggt
    31501 taacttgtcc ggtacaagtc gccagccgtt atatttcgat ggaacatggt ggtattcagc
    31561 caggaagcgt cgtatatcgt ttttcatttc ctcgcatgag ttctgatcat gaagtgcagc
    31621 ggaaagccag cgtccgatat attcagggat tcgcacatca agtacttttt ctgccgtcgt
    31681 cagcattgcc tgtcggcagg cgttccagcc gttgacatag tgacgtgttg catcccatga
    31741 aacctgcaca tcagggtaag catcctgttc tgttctcact ggcggtatct gaggaacagt
    31801 gaatagtgga ctgacgatat atttatccag agagcctccc cgctcttctg caagactttc
    31861 ccacgatgtc attgtgggga ccctgtaacc tgcttttttg tgcgtataca tccatgccac
    31921 tctgccgtta tcatctgcgg cggcaatgcg ctgccaggca ggataaagcg gggtgtatgt
    31981 ttctctgtca ttacgggcgt caggcatggc atcaatactg caaacttccc ccgtaaggtt
    32041 attcagccag gccttcgggc taatctctga cgttacgatt tcatccagtt ttttctccag
    32101 attttctatg tgactgatta gcgcaaggat aactggcgga gtggcaaggg agtgaaatgc
    32161 ttccagatca catcccttgt ctccatatac ggtattttgt gcggcgtcac gcagtgcttt
    32221 cagatcagct tctttcatcg ttcagactcc cttgttgctg ttttgtaggc gcgcaggatg
    32281 tcgcgtgttt ttcctgataa tgacgactta accaggaaaa agccgctccg gctttctgtg
    32341 atgtccggcg tgcacagaag taccgcatca acgacacgat tatgttttcg caatgtcagg
    32401 cgggaactgg tgataatcag ttttgcctgt tcgccgtaat cggtgaatga aataatcatc
    32461 tgatggcgat ctccgttatg aaagtgttcc tgctggttca atgctgataa tttctgctgc
    32521 aataagcata cctgccagcc atgcttcgcc gagcatgtcc tcatccgtac atgcaagatc
    32581 accgatctgt attgtggcca taatgttagt ttcaccggta tattcatcct gtgcttcttc
    32641 atacggcagg ttttcataaa ggttttcaat tgcgcaggtt attacatcca gtcccgtcag
    32701 attgccgaca acgtccactt caaatgtttc gcggtactcc cattgcccga gtgtcagacg
    32761 aaccgtctgt cttgccatgc gcccgcataa tgtcatgttc gggtcgtagt tcatgattgc
    32821 cgcagtagtg ctattcatgt tctgcctccg gtgtgtaaat agctttgtcg tgtttaaatt
    32881 caccgttcca gtttttcttc atagggagtt caccgttcat gtaaaactgg tataaacggt
    32941 ggcatccttt ttccagcaat acgggggtga atttcgtgaa tgttgctttc ccgtgtggag
    33001 tgatctgtac tgcttcttcg gtcaggtatc tgtcacgggc ataagaggcc gtgcgccagc
    33061 ggggtgtttt ctccggatcg cgttgttcgt tgaataccca ttttcggcca gacagccacc
    33121 acattatctg gctggtattt acgccgttca gcgcacggca gaactgggga acagtcatgc
    33181 ctttagagaa atgtttttcc agactgtcta cggttgcaga gagcgtttta ttttccaggg
    33241 ccatagcttc agcccgttct tcggcctcaa taaccatcaa cgcgagttcc ttgcgagtga
    33301 gtacgcgctg aacatcgatg ttttcccgct gagtgaagta gaactccaca aggtcttcgt
    33361 gatagttcca cgcctgatcc gtttccagca attttgcgtg gttggctgcg ccgcgttcgg
    33421 tccagaggat gagggatcga acattgctgg caattttcac agagtagctt tgagatactc
    33481 tgtgcttaag ttcacgtagt tcatcgcctt ccagcaaaaa ataatgcttt ccaacaacga
    33541 atcgttcttc gtttcgggaa tgattctggc gaatacggat tggtgttgct ccataacctg
    33601 ccgccagttg ttcagtggtc acaacgcgtt gaccgcgata ttcgataatt tgcaaatcac
    33661 gggctgccac cggcacaagt tcagttgatt ttttcatcat tacacctcac gaaaagctga
    33721 ttagtggaac actctggttt ccggttctga gttgtcagtg gcttcactga taagcccatc
    33781 cagaatcatg tgcatcgctc tgtaaccttc cattgtgatg ccggtttttt cgccgtcgcc
    33841 cagagcgccg gtgaatgctt tgtacataag aatgcttttg agcagccctg attccgctcc
    33901 gtaagtttct atgagtgccc attccatata gttctgcata gcgagacgta atggtttcag
    33961 gtagattgtc atgccaccgt tgtggcctct gtacatggtg cccttgtctg taccgccatt
    34021 ctcgttaagc acttcggtcc agccgtttct ggtgagctgt tcagagaaga acatggcggt
    34081 ataaagccag cgccagtaca tgattgtctt ttccggcgct agctcacctg tcatgccttt
    34141 ttcttctgct tcactgatgc aacacactat ttccagacca tgccagatcg gctcgtcaaa
    34201 acagccttca tcgagcattt ccagtgcttt gttgatatca agcagtccgt aaggtgtgtg
    34261 gataccttca gcggtaagaa aaaataactg ttcagttgca gtcatggttt gctctcttct
    34321 taaatggtct ttgcgcgaca agccggcaat actgatcaat ccaggcgttg tatttttcca
    34381 tccaggcatc aaactgtttt ctgcgtgcaa ggatgcgcaa caggcgatga aaacaacgcc
    34441 cgtgagctct gtagtattcg ggcgtaattt ctcccggatg aaagacttct ccatattccg
    34501 gatgcatcag gcgctggtca ggcagacgtt tttcaaaacc tgaactttcg aataccttcg
    34561 ttgtcataaa atgagcgaga tgtatgatgg ccgtttttcg cgtgaagcat ttcttaactt
    34621 gtccgtggcg gcagactctg taaacagggc cgcaatcgag ttcgtattta tgaaaagcac
    34681 ggtcgattcc ggcattattt attgcactgt attccgcatc actgaagaaa ggtggaaata
    34741 cgatgtatcc ttccattgtc agcgccagaa cgcgcggatc ggtttctgtc agccattcgt
    34801 tttctgtgaa ttcttcagaa ttaatttcaa tgatttcagg ttcttctttc atggttactc
    34861 cgtgttcatc cggtgttaat tacttttatt cactctgagc gcagccagaa gatgtggctc
    34921 agcaattcgt ttgttgcgat atatctgtga tacagcctgg ccttttcagg tcgtaaaaat
    34981 ccccgacaat aaagctgtaa attaattact gttcttaaat aatgttattc gttttcagtc
    35041 cggcacagat gcttttctgc tttgtctgct gtacaggata atgagcggaa aagacaattc
    35101 atcatgttta gcgcatctgg tgaaccaata tcaccgctgt caataatcac ctgtaacaaa
    35161 gacaggcttt ctgttatctc gcagtggaca ttatcgagta cttcaacgca attattcatt
    35221 tttcattatc tccgttattc cgggggagga gctatgctgt taatgattaa cgcatccggt
    35281 aatatgcccc tctttaatgt aatcgatgca ttcattcaaa agttcatcaa tgattaactt
    35341 tcctgattca gtcagatact caccatgttg gttgataccg acagcatcct ggtatgcagt
    35401 gcgtattgct gtttcaccat ctttccgccc aaactcacca cgggtaacgc cttcaaatcg
    35461 taatagcaac tggcttatga actgttctgt tatttcaata gttgttaact ctccatcatg
    35521 acgctttaca gtgaggagat tacttcctgt ttttcgtttc atccgccgca gtgctgcaac
    35581 cgttatgcgg cgacgatatg cagaaatgta ttcatctttc atttgttatt tcccgtatgc
    35641 ctttttgaga aacaatactg caactgacca gtatccggca tcagccatta ataaagcggt
    35701 tttataggca tgtttgtttt tcatgtgtca tccccatttt aaaatagtga gaactcccga
    35761 ccgcagaccg tcatgttttt atgtatgatt gtctgtttaa tttcctttat tcgttctcag
    35821 agcatcaaca tactcgtaag ccttttcaca ggttttattc atggaacgaa tcagacaaag
    35881 taagaaatca tctgtctctc cgttatcacc agagttttta aatataaact caagcattga
    35941 cgtgttttct tttatttctg ctgccacttc ctcaagcata atcaggggag ttttcatgtt
    36001 ctttgttcct taaatgcatc gcatgcgctt ctggcgtatt gttgtgccag taaaaatatg
    36061 tcatccgaaa gttcatcaca ttcttcatca ccggaagccg aaatgattaa ccccgcttca
    36121 agcagtactg caatgtgatg aaaagctgtt tccggttcgt tggtgaggcc tttgaacatt
    36181 ttcatcttac gattcctttg tcgtgtcaga atggaaactt gtaagtaacc acatcggatc
    36241 gcagtcaagt atatttgcca gaggaatcac ctggcttgcc agaggttcag ccttgccgtt
    36301 ctcccactgg atgatggttt cttcatcaac tccaagcagc gtggcgagtt cagcggtgga
    36361 tagaccgcag ctttcgcgtt gggtgcggat gccatcacgg caatcttttt tgtttctcgg
    36421 aggtaacaga atatgtttta gttgttggat tttttcgatt ccaacctcaa ggagtacgtg
    36481 acggtttctt tccgatacag actccagact ttcatgaagc agcgattcta cttgttcgat
    36541 aagcatcaat tgtgaagtag acataaaaaa acctcgtaac caatatttcg aaatctaggt
    36601 tacgagtgag taatgtaggt gtcaagagca attaatgttt tttgttgaat taaatttgca
    36661 tacgcatctc aacgacaaca ccaataattt cacaatcccc attgatgggg atcagtggat
    36721 agcgtgggtt tagtggcttt aggaaacatt gcccagcgtc ttctatgtac tgtttaaggg
    36781 ttgcctctcc ggagtttttg agcctggcga cgacatattt tcctgaaaaa acttctttgt
    36841 cagggttaac aagaatgttc atgccttcaa aaatagagaa ctctccattc tgtgatgtca
    36901 ttgagtcccc tttcacttcc agccagaagc cgtgatcgcc cgcatatata tcgctaccaa
    36961 tccattcttc tggataagct atgtaatctt cgcagttcca ggggccagca ctcacccatg
    37021 ttagtttcgg atatctataa cggtttggag aaactggctt aggatttcgg acgttttgaa
    37081 tgctatctgt aggtgatggg gcgctgccgt caagaagcca tttaggagag catttcaatg
    37141 catccgcgat tctaaatagg tttgaagatc ttgtgtcttg tgagtctcct aactctattt
    37201 tgctgattgt tacacgcgag acaccagtca attttgccag gtcatcttgt gacattccta
    37261 gctgtactct gcgcattaac agtctgccac caaagctatc gtgatccatc ttctccccct
    37321 tcttgtcgaa ataatcgttt cgaaacaaat atagcaaaaa atcttgaaat ctaggtttcg
    37381 gaaaattaag attaatcgaa acaaacgtgt cgaggtggat atggatttgt atgaaattct
    37441 aaaaaacatg tttggatcga atgttgaaat aggtcgttat tttccccgaa ggggaagggc
    37501 tagaaccgga caggctgtag gtaagtggaa aacccgaggt gtgccggaag acgtggcgat
    37561 cctttgtcat ctggatccca aaattcctta tcagcatccg tcgctcatga atgccagtca
    37621 tgaatcgtga tgttctgccg gaggctgtat gtcccctgat tacgttcaga tggagatgcc
    37681 tgccaggtac agccaggcag attcagagtg gattaagcgg cagttacaga gcctgccttc
    37741 ctcactgaga cagaaggttg ccctgaaata cgcggaggtt tacgaagtta cttttgattc
    37801 cgagcctgtt tcattccgta aggagaacag agcgaggcat gaagccaatg tacggctgcg
    37861 caggttcgtt gaaacacacg ggcgtgcatt acaggggtat acgactcagc cgcccctggc
    37921 aggaacgcaa cagcgtacct gaatggtgtt ggtcttaaag gtgaccgacg ggctgagacc
    37981 ccacttctca ggatgcacct gtgtactacc aagctagtgc ataagttgag agggggggta
    38041 agggggggag tgcccgtgtg ttagtgcgaa gcactggaac gggatcttcc aacagacggg
    38101 tacagaggtt aggtagatct cgatctaaag gggcatccct gaaaaaacgg acgtatcagc
    38161 aaactagtac atcatggtta aaatatgaga gctgaactga aacaggtact aatcaacctt
    38221 ctggaagagg aattcggaca tggaatggac aaggtcacat tcgactacgt tctgcaaaag
    38281 aaaatcaaat ctctgggctg tgagttgcaa cgctgtttca ctgtcaggct gaaaggtgat
    38341 cgcaggggat ttatcgatct tctggttatt tctcccgatg gtcagcgttg cgcagttgag
    38401 gtggataacc gatcgccccg tcagcgttca ctgatgaaaa ttcgagcgct tcctgaaggc
    38461 atttcgggat ttgtttttct ccgtgatggc aaacatccac agcgttatat cgccgatggt
    38521 gtggatgtta tccgggcgac ccggtttaag taatcactgt atcagccctg tcatcgggag
    38581 aataccatgc tgaatatcaa gccgaatttt gctcaggaac gtgcgctgaa tatgttgcgt
    38641 catgactgga aaacatatca gtccttcatg atgtacatgc ctacagggag tggtaaaacc
    38701 ggcctggcgg cgttcatcac tgctggtctg gtaagccgtg gcatgcgcgt tctgtttgtc
    38761 gttccttata ccattttgat caaccagaca gcacagcgct ttgttcagta cgggctttcc
    38821 ggggatgaaa tcagctttat ctggcgtgat catcccaata acgacccgtc acgactgatt
    38881 cagattgcca gtgcggatac gctgattcgc cgtgattttc ctcagaatat cgacctgctg
    38941 attattgatg aagctcatct ccgtaagcgc cgcatcctca cggaaatcga acgactggtt
    39001 gcagagggaa atgtgaaggt gattgggttg tccggtacac ccttttcatc gttcctgggg
    39061 cgttattacc agcgtcttct caagccgacc actatagggg aactgatcca gcgtggcgaa
    39121 ctgagcaatt acgaatttta tgcgcccacg aaaccggatc tggaaggtgt aaaaacgaaa
    39181 gtctcaatgg agtacggcag ggattacgac gaatcacaac tggcagagat tatgtgcggc
    39241 gcggatctgg ttggcgatat tgtcgataac tggctgtgta acggaaggga tttaccgaca
    39301 gtggcgttct gcgtcaataa aacgcatgcc agcttcgtga ccatgcagtt caacaaagcc
    39361 ggaattaacg cagaggtgat ggttgctgaa acgccacatg atgagcgtca gttaatgatc
    39421 agtcgttttg aaacgggagc gacgaaaatc attgtcagcg ttggcgttct ggtggcggga
    39481 tttgacagtg atgtccggtg catcatttac gcacgcccga caaaatcaga gattcgctgg
    39541 cttcaggcga ttgggagagg attgcgtaca gcgccaggca aggagtcatg cctgattttc
    39601 gaccattccg gtacggttca tcgcctggga tttcctgaaa atattgagta tgacgaactg
    39661 cccggcaaaa acgacgggat gcaggtgtca tcatcacgtg gtaacgaaat tcgggaagaa
    39721 aaactgccga aagaatgtcc ggggtgtcat ttcatgaaac cggcaggtgt ttatgtctgt
    39781 ccgaaatgcg gatttaaacc gctggctggt gaggatgtga acacagatac ccgccgcaat
    39841 atcaggaaac tcagcaagga cgaaaaaatt tacaccaaaa gcgacaaaca ggcctggtgg
    39901 agtcagatca aattctatca gcgtcagcgt gcctctctgg ggcgaccggt cagcgatgga
    39961 tggtgcgctc acactttccg ggagaagttc ggcgaatggc ctgacgggct gagcagtttt
    40021 ccgatggaaa tcaccccgga ggtcagtaat tacatcagac acaaattcat ccggtttgcc
    40081 agagggcgta gccgggcaga gaagatgacg gaaaaatccc ccgaagcgct ttctctgtcc
    40141 ctgcttcatg gtgtccggtc tggggtgccg gaaggcagtg agtcgtggca gatcatgcag
    40201 gcaaagcaac aacttcagaa aaacagaaac agtctgagtc agtaagatga aaacagcaga
    40261 agcagcgaaa ggccactggc ctgaaatttt aaaacattac ggactgccgc cagtcacggg
    40321 taaaaagcac ttcaaagggg aatgtccggt ttgtggtgcc agaggtaagt tccgtattga
    40381 tgaccgtgat ggcaccggaa cgtggatctg tgtttgtggt agcggtgacg gcatgaaact
    40441 gatcgctctg acacaaaaaa gagcgtttca tgaaatttgc gctgaaatag accgcatcac
    40501 ggggaatgaa taccggagag ataaaccgcc tgtgatctgt acgtcggaaa gcctgagatc
    40561 gcgcattcag cgtcgatttt cagcgctcac gtcgctacag ggaacatctg gtgctgaata
    40621 tcttcgttcc cgtggcatat tcagtcttcc ggttgaggga gttcgcttca acagccagca
    40681 aaattataac gggcgcatgt tccagtctct ttatgccctg gcgaccgacg acaagggaga
    40741 actgtgttac ctgcatcaga cattgcttga tggagctaaa aaagcagaca ttggtatcag
    40801 cgccagaagg ctcaaatctt tacaggacga taactatctg gaccatgccc ggtcagttgc
    40861 tatcaggatg tttccggtag ccagtacact gggtatagct gaaggtatcg aaaccgcgtt
    40921 gtccgcacat cagatttaca aagtaaatac ctgggcaacc attaacagtg gttttatgaa
    40981 aaagttccgc gtaccggcgg gagtcaccca tctgattatt tttgccgatc gcgatgaata
    41041 cagtgctacc ggaatggctg cggcctgtga atgcgcacat gcaaatctcc aggcccgaaa
    41101 tgacattcag cgggtaagtg tccggtggcc tgatcatgat gatttcaaca atatgctcat
    41161 gaacggcgat caggttcggg aactggtttt ttacagaaaa aaggcggttg cgtgatgcgt
    41221 acggacaatc aggtacataa agctttattc actatcccga cggcagcgta cagcgccgtt
    41281 ccggcaaata tcaaacctct gccagaacaa aggaggatca ccggacataa acagacagat
    41341 gcttatctct ggattctgga ggttattcac ctgaacgagg ccgtacatct ggacgcagcc
    41401 gaagcagcac tggagaaact caaaataacg ccggaagaag cgagtgaacg gtatggacgt
    41461 tatttgcagg agattaatgt tgatcctttc cagattgctt tcgcaaccat aggcatagat
    41521 aacccggcgc aggcgatcag gaatgcccgt gagaatatca aaaaagccgc atcagtcagg
    41581 gccacatttg gcagctatga ggcagcactc gatgatgtgg aggctgagcg gataattcgc
    41641 acctccccga aatttatcga cgattactac tggggctgga ctgcggcaga gaaaaaagcc
    41701 ggaagtattg acggtgttcg ctcaaatgaa attgatgatc agcgtcgtgc atatgttgat
    41761 ggctatcgtg atgtactgcc agagcctcat acattgtcag acgttgttcg tgagtttatt
    41821 tactgggact ggctttatga gatgcgccag acagcgggga gagaaacagg ggacaaatac
    41881 ggtttcacag gtgaacatca tgagtcagta tatgatcggc agttctggct cgaaaatttg
    41941 ctgggaaaaa taaagcctgt gacgcgtgat gaagcggttg aagtgtgtcg ctggtttctg
    42001 gcaagcggaa aagatgagta catggaagac aatggttcag cggtcattct caatctggtt
    42061 ggggagtgtg agcaatgaag ccggagatat ccagtagaca ttttccccct gagcattcaa
    42121 atcagaaaaa gtatggttac agcggagtac agaataatgc gtgatatcca acaggttctg
    42181 tcccgatggg gtgcatgggt ggtaaataat catgagagtg tggcatggtc cggcgtcgct
    42241 gccggattta agggagtgat cccatcaaag gttaaatcac ggcctcagtg taccgatgat
    42301 gatgcgctga ttatcagcaa ttgcatggcg caactgaacg tcaacaacag cgatttgcat
    42361 gattttctgt atgattatta tgtgttcggg atgacgctta tgggactggg ccgtaagcat
    42421 gagcgctctg attgctgggc cgggcgggta ctgcaaaaag ctgaaggtgt tattgagggg
    42481 atgttgatta tgcagggaat aaaactggaa atggacagat acgttgagcg tgaacgatca
    42541 ggggcacagg tcagtcagtt ttccggacat acgggaaatt gaaagcgcgg ggttttactg
    42601 tagaatgact gcgggtgctt gagggtgtct gcctcgggca tgctgccgta aggcagacgg
    42661 agaaaagccc cagttaacat tacgcgtcct gcaagacgct taacattaat ctgaggccaa
    42721 tttcatgctg aacacatgta ggttagcctc ttacgtgccg aaaggcaagg agaagcaggc
    42781 tatgaagcag caaaaggcga tgttaatcgc cctgatcgtc atctgtttaa ccgtcattct
    42841 gacggtactg gtagcgagga aagacctctg cgatgtacga atccgaaccg gccagacgga
    42901 ggtcgctgtc ttcgtagact acgaatctga gaagtaagag acctggcggg ggagtaatct
    42961 cccgccccct ctgatgtgtc aggcatcctc aacgcacccg tactttagtt gtcatatgct
    43021 ccgcaaaatg caggttgtcg ttgcaacccg tgcggtaatt ttttgtgtgc gagtgctcaa
    43081 aaaacgttga ttttcataaa aggacgattt tatgctgcca gaccagcgac cgtaaatcaa
    43141 gaaagtctgt aaaatctgtt gaaccagaca ttgccgaccg ggctaacaac tggctcaaat
    43201 cttatggact tgattacaaa ctggagcagg caccacttaa caacgagatc gataacgcac
    43261 tggatgctta tttttcgaaa aatggcggca caggcggtaa tcgtcccgac gtaaaactcc
    43321 ttgttcagga caaatacggc aagcagtatc ccgttctcat cgagtataaa ggctacaagg
    43381 acaggcttat cagacttggc agcaacggta ttattgagaa caaagatgcc agaaaagaat
    43441 ggaattttaa aaatattaat ggatacgcgg tgaatggcgc agtccattat gccaacgcac
    43501 ttcttcagtt caccaattac cctgatatta ttgctattgg tatgaccggc tggcgcgatg
    43561 atggtacagg cgaactgcat catgaaatcg gcgtgtggta cgtatcaaag aataatttag
    43621 gagccgggca gaaggttggt gaattcaccg acttatcgtt ccttgctgac aaaaatgttg
    43681 atggatttct caacaagata aagctgctta atcttcctcc cgaagaactg gaaaaaatca
    43741 aggccagcaa agaggaagag atagacacgc gactttcccg tctgaataac gatatttacc
    43801 agaatgaaaa ggggcttggt gaaagcgatc gtgtttacct tgttgtggct accgttattg
    43861 caacgttggg gattccggga aagttagcac cgctggataa aaaagaactg acctcttcaa
    43921 cggaggagga cttgcgtgat ggtgatatta ttttcaggaa aataaggaat tttttaaggc
    43981 tgaaagctgt tcctgaaaca aaaagagaaa tgattttgcg ctcgttgcaa aatacgctgt
    44041 ggactgaaaa cattaacaaa ccagtcaacg gtgaaagcca gttaaagcgc gtgtttgtta
    44101 aagttgttga tgatcttggg gagtattaca aaatcggact gaccactgat tttacgggca
    44161 aactgtttaa tgagatgtat cgctggcttg gatttacgca ggacaaactc aatgacgtcg
    44221 ttctgacacc accttatgtt gccacattgc tggcacgact cgccagagta aacaaagatt
    44281 cctatgtgtg ggattttgct acaggttctg cgggattgct tgtggctgca atgaatgaaa
    44341 tgctcattga cgccagagaa aatattcact caccgaatga actacaactt aaggaagcgc
    44401 aaatcaaggc tgaacagctt cttggccttg aggtgttatc cagtatttat atgctggcca
    44461 tcctgaatat gattttgatg ggggatggta gctcgaatat cctcaataaa gactcgctag
    44521 ccgattttga cggcaagtat ggatttggga aaacaggaga aaaattccct gcggacgcat
    44581 ttattctcaa tcctccgtat tcagctaaag gtaacggcat gattttcgtg cagaaggcgc
    44641 tgtcgatgat ggataaaggc tatgctgcag tgattatcca gagttcagcc ggcacaggaa
    44701 aggctacgga gtacaacaaa aaaatactca aggaaaatac cttgctggca agcatcaaaa
    44761 tgcctgcgga tctgtttatt ggtaaatcaa gcgttcagac gtacatttac gtttttcagg
    44821 taaaaattcc gcataacgcg aaacaggctg ttaagtttat cgacttttcc aatgacggct
    44881 atgctcgctc taatcgcaaa aaggcaagaa acaatcttgt tgatgccgat cgtgcaaaag
    44941 agcgttatca ggaggttgtg gatctggttc acttcgggaa aggttgcctt aatatcttta
    45001 cggaggatga atattttgag ggaacaatcg atcctgatag tggtgaggac tggaatcaga
    45061 ccaggccagt agatgctagg ccaacgctgg atgatttcaa aaagacggta ggtgattacc
    45121 ttgcatggga ggtctcgcaa ttactgaaaa aacaaggtga aaacaatttt gcgggaaagt
    45181 aaattccccg ctcagcgata aattaagaag cgttgagtgg ggagagttca ggattggcga
    45241 tttgttctat gtgagcacag gctcgcttat ctcctcagga agattaaaac caggcaaaat
    45301 taaacgtgtt tcagcaaaga gcgatcaaaa cggtatcatc ggagagtttg ataccgagtt
    45361 tatggatgat gcaaggcatt atgaaaattt tataagcgtt aatttttttg gagatgcttt
    45421 ttatcatcct tattgtgcca gcgtagaaat gaaggtgcat gttcttacat taaaatcaag
    45481 taagtccgac tttaatgcaa agcgcggact gtatgttgcc agtatgatta ataaagcttt
    45541 aaaggggcgt tttaattatg gtcaacagtt atcgagttcc aaactcagag atggagaata
    45601 tttcatctca ttaccagtga aaaataatga aattgactgg ttttttatgg ataattatat
    45661 agaagagctg gaagcggccc atatagaaga gctggaagct tacttgctgg ttagcggact
    45721 cgcggactcg cggactcgcg gactcgcgga ctcgcggact cgcggactcg cggactcgcg
    45781 gactgataag ctatgagctt acaccgcagg aatataacgc gattgagcta ttaaatagca
    45841 ataatcttat gtggggcact tataacctca aaaagttatt tggccccgca ataagaggta
    45901 agcgtctgaa aaacgcagat cgtattcccg gatccttgcc ttttgttact gcgggtgaag
    45961 gcaatgaagg tatttctgca tttatcagta atgatgttgt tgtattccct gaaaatacca
    46021 ttacgataga tatgtttggc tccgcaaaat acagaaatta tgagtatggt gcagatgacc
    46081 atgtggcagt tgttcataca gaattctttg acagaaatgc cgttattttc ataacagcat
    46141 caattcataa gtcttcttgt accggaaagt tcaactacgg gagaaatttc tatgcaaaag
    46201 atgccgacga gttaatggta tctttgccgt caaaagataa taaccctgat tattctttca
    46261 tggagacgct aattttagca gtacataaac tggtcatcaa agatgttgtc cagtacactg
    46321 ataaaaagct ggtagcgtac aggcagatta catctgagaa ggtcgtttag tgcaaatccc
    46381 cacctgacag ggtggggaat ggtcaggtta tcgtgccctg atgcagaaaa acagtttaca
    46441 atcgtaaaaa tcatcatatc atgacaaaag tggttacatt gccacgcagc cgaacccgcc
    46501 gatgcgcggg ttttttgtat ccggaatcct gtgagttata cggaaagtac acagaaagga
    46561 aggtgcgacc gcaattaata acaaaatctt aaaaattgca tatggcacta ttagttttct
    46621 aaatattgta tattttgagt attgcaggat atcccctgta acgaagtttg cgtaacagca
    46681 ttttactcta cgagtttgcc agcctccccc agtgggctgg ctttttttat ctgatgcgct
    46741 tcacatcagg gtgacgttct gttgccgctt catggaaaat gtcgctgagc cagcccggta
    46801 tctcgtcttc tgtcatatcg tctggaatga caatatcgta taaccgggag aattttcgtt
    46861 ctgtcaggtc tgcccggaag accagccagt gatcaccttc acgtttgaca ccaatatgtc
    46921 gaccaaagac gttgtagatt agcactatcg acctctttca ggaattgtgt aactgagatg
    46981 acagattcag atttcgtcaa taaatgcaaa atgtcaatcc tgagatcatg gtgagctatc
    47041 tggcggtttt ttatatctgt ttaacggggg atgttacata cctcacaatt cagtcagttg
    47101 aaggttgtct gcccggatga gaatttgtta gaaaatttct gcatggtgaa tccccctgag
    47161 cggaggggca tatcagcgac aggtggttgg ttatatccct tatctggtgc gggttcaggt
    47221 gctgatactg aactcaccgg gaggcacccg gcaccatgca atgaacggta catagcgcta
    47281 ctcttcagcc cctctccgga ggggcttttt aatgagggga agacactggg cagtgaaatg
    47341 ttaaatcact cacaattcag gtagttgacg gttgtctgct tggctgagaa tttgtaaaaa
    47401 aaatcctgca tggtgaatcc ccctgtgcgg aggggcgact ggtgaatggt atgatctctt
    47461 tgatgatcgt aagcgagaat acgcgggttt ggtggcacca ggccgaactc accgggaggc
    47521 acccggcacc atgcagtata cagagattag gcatatacca aggcccctca tagcaggggc
    47581 ctttttacat gcaaaaaaag ccgccccggg aagagcggtt ggcaagaaga aaagcaatat
    47641 gaacaattaa ttaacgatgg gaataatacc ttagagtaat caccttgcgc aactgtaagg
    47701 gcatatttca tctttgcggg ctgtttttcc gggtgacttc tgtgtttccg gaggccagcc
    47761 tgtaccttct ctgactcaga acattatccc ggccgggagg attcatggca tttaaacact
    47821 atgacgtggt cagggcggcg tcgccgtcag accttgcgga acgaataact caaaaactga
    47881 aggaagggtg gcagccttat ggtagtgcgc tgatttcgac agctggttat ggtgcggagt
    47941 tcatccagcc agttgtgagt gaggggagca tctcatcacc agaggagcca ggcaaccgtc
    48001 cgacgacctc agcgccttct gttgcgccag aatattacta tgtgatcgcg cttgctggtc
    48061 agtccaatgg tatgtcatac ggtgagggac tgccattacc ggatacattc gacagccctg
    48121 atccacgtat taaacagtta gcgcgtcgca gtacggtgac accgggaggt gctgcatgta
    48181 tctataacga tattatcccg gcagatcact gtctccatga tgtggaggat atgacagggc
    48241 ttaatcatcc caaagcggat ttatcgaaag gtcagtacgg ctgtgtggga caggggctgc
    48301 atatcgccaa aaagcttctg ccatttatac cggcgaatgc gggcattctt cttgttccgt
    48361 gctgccgtgg tggttcagct ttcaccaccg gaaccgatgg cacatacagt gacactactg
    48421 gcgcctcaga gagttccacc cgctgggggg tgggcaggcc gctgtataag gacctcattg
    48481 gtcgtacaaa agccgcgctg gcgaagaatc cgaaaaatgt gctgcttgcc gtggtatgga
    48541 tgcaagggga atttgatttt aacggaacgc cagcgaatca tgcagcccgt tttacagaag
    48601 tggtggatca ataccgtgcg gaccttgcag atatggcggg gcagtgtgtg ggtggctcag
    48661 ctgacagtgt tccctggatt tgtggagaca caacatattt ctggaagcag aagaacgaaa
    48721 cggcgtacca gacggtgtac ggtagttaca aaaataaaac ggaaaagaat atccatttcg
    48781 tgccgttcat gaccgatgag aacggggtga atgtgccgac gaacaaaccg gaagaagacc
    48841 cggatattcc ggatatcggg tattacggtt caaaatggcg aacggacaga agcacctgga
    48901 catctcagga cagggccagt catttcagtt catgggctcg ccgtgggatt atttccgacc
    48961 gtctggcaac ggcgattctg agctgcgcgg gtaagtcttc tgcgtttgtt aatggtactg
    49021 ccggggtggt tgttccagac agaccggtta ccacctcaga gtctgtaatt ttttacgatg
    49081 ccaaaaaagc ttcagacaat cagctgaaac cttatggctg ggacggtatg gatggcagac
    49141 gcacactggt tgatgacagc ggcaataaag ctctgcgaat tgagaaaaat aacagcgcga
    49201 aatcctggtc aatgtactgt gatattgctg cagacaaggc aaaactttta ctggaaaaag
    49261 gcggggaaat tgctgtccgg tttaaaatcc ccgaaaacgt caatcttgag acaaccagaa
    49321 acaagtatgc ctttggtttg tactggcgaa tagcggaatg gccgggtgag ggtggtgaag
    49381 gctatctgag ttctttcttt gtccagacag ataaagccag tattgatgtt gcataccatc
    49441 atacagttaa tcaacaaaaa gaacttggca cgtttggcgc attcgaccat gactggcata
    49501 cgcttgcatt taaatttaag ggcagtaaca gcattaatgt tactccggtg cttgatggtg
    49561 tggatggaca ggcgtttgac ctggtgaaat gggccaatac tgctaatgga ctcaacaggt
    49621 ttgtcattac ggatattaca ggtagtgcag aaacctaccc tgtacttatt gatacggtgg
    49681 aagttaaagc aaacaaagct ggagcagccg cataattgct aaaaaaagcc gccagcggca
    49741 ggaatggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat gctttcgtac
    49801 attggttttt taaatgaaaa cagttctcat tgtcaaccat aacggtaaga aattatgata
    49861 tttattcatc aggtgatgct gtacttctgt acggcggtct gtgtgctgta tcttctttcg
    49921 ggtgggtaca gggcagtgcg cgatttctgg cgcaggcaga ttgataaaag ggccgctgag
    49981 aaaatcagcg ccagtcagtc agccggaagc aaacccgaag agcccgttac tccttaacaa
    50041 cccctttcag cgagaaaatc ccatgtcaga aattacatcc ctggtcactg cagaggcagt
    50101 gaaggaagtc ctgcgctctg aagaagtccg gagcgcactg aaacagaaac ttcgccagaa
    50161 tcttgaggcg cgtcttgatg cagaagttga tgccattctg gatgagctgc ttggtgtaca
    50221 ggcagagcca ccgactgaag cgggagatac caccgcagag agcggtgaag ttcagcctga
    50281 atcaccggtc gccgatgcga ctgaacctca acccgaatcg gtcatgatgc tgtaacgagg
    50341 agtcagggcc atcagtaaac ggctgctggc ctttttcatg ttgtgagctt ccgg
//
LOCUS       DAZGCQ010000027        50381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000027 DAZGCQ010000000
VERSION     DAZGCQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.027"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHK07_001666"
     CDS             complement(<1..150)
                     /locus_tag="ACHK07_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO8102011.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINN"
     gene            complement(235..555)
                     /gene="chbB"
                     /locus_tag="ACHK07_001667"
     CDS             complement(235..555)
                     /gene="chbB"
                     /locus_tag="ACHK07_001667"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO8102012.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(854..1192)
                     /gene="osmE"
                     /locus_tag="ACHK07_001668"
     CDS             complement(854..1192)
                     /gene="osmE"
                     /locus_tag="ACHK07_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO8102013.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1394..2221
                     /gene="nadE"
                     /locus_tag="ACHK07_001669"
     CDS             1394..2221
                     /gene="nadE"
                     /locus_tag="ACHK07_001669"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO8102014.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2451..3338
                     /gene="cho"
                     /locus_tag="ACHK07_001670"
     CDS             2451..3338
                     /gene="cho"
                     /locus_tag="ACHK07_001670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO8102015.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3298..3873)
                     /gene="ves"
                     /locus_tag="ACHK07_001671"
     CDS             complement(3298..3873)
                     /gene="ves"
                     /locus_tag="ACHK07_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO8102016.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4076..4561)
                     /gene="spy"
                     /locus_tag="ACHK07_001672"
     CDS             complement(4076..4561)
                     /gene="spy"
                     /locus_tag="ACHK07_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO8102017.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4891..5859)
                     /gene="astE"
                     /locus_tag="ACHK07_001673"
     CDS             complement(4891..5859)
                     /gene="astE"
                     /locus_tag="ACHK07_001673"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO8102018.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5852..7195)
                     /gene="astB"
                     /locus_tag="ACHK07_001674"
     CDS             complement(5852..7195)
                     /gene="astB"
                     /locus_tag="ACHK07_001674"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO8102019.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREKGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7192..8670)
                     /gene="astD"
                     /locus_tag="ACHK07_001675"
     CDS             complement(7192..8670)
                     /gene="astD"
                     /locus_tag="ACHK07_001675"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO8102020.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAVFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPHLLQAGTSLLTPGIIEMTCVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8667..9701)
                     /gene="astA"
                     /locus_tag="ACHK07_001676"
     CDS             complement(8667..9701)
                     /gene="astA"
                     /locus_tag="ACHK07_001676"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO8102021.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGIVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9698..10918)
                     /gene="astC"
                     /locus_tag="ACHK07_001677"
     CDS             complement(9698..10918)
                     /gene="astC"
                     /locus_tag="ACHK07_001677"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO8102022.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACERFVSGGSS"
     gene            11364..12170
                     /gene="xthA"
                     /locus_tag="ACHK07_001678"
     CDS             11364..12170
                     /gene="xthA"
                     /locus_tag="ACHK07_001678"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO8102023.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12337..13047
                     /gene="ydjX"
                     /locus_tag="ACHK07_001679"
     CDS             12337..13047
                     /gene="ydjX"
                     /locus_tag="ACHK07_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8102024.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            13052..13729
                     /gene="ydjY"
                     /locus_tag="ACHK07_001680"
     CDS             13052..13729
                     /gene="ydjY"
                     /locus_tag="ACHK07_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO8102025.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VIFKITE"
     gene            13744..14451
                     /gene="ydjZ"
                     /locus_tag="ACHK07_001681"
     CDS             13744..14451
                     /gene="ydjZ"
                     /locus_tag="ACHK07_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8102026.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14451..14999
                     /gene="ynjA"
                     /locus_tag="ACHK07_001682"
     CDS             14451..14999
                     /gene="ynjA"
                     /locus_tag="ACHK07_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO8102027.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            15009..16175
                     /gene="ynjB"
                     /locus_tag="ACHK07_001683"
     CDS             15009..16175
                     /gene="ynjB"
                     /locus_tag="ACHK07_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8102028.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VPPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTADPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKSGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            16148..17683
                     /gene="ynjC"
                     /locus_tag="ACHK07_001684"
     CDS             16148..17683
                     /gene="ynjC"
                     /locus_tag="ACHK07_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO8102029.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLMIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVILLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDRSWTAVVWGHLLWVIPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17683..18336
                     /gene="ynjD"
                     /locus_tag="ACHK07_001685"
     CDS             17683..18336
                     /gene="ynjD"
                     /locus_tag="ACHK07_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8102030.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALPGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDFQDVPADSSVLDMAQWSENYNKLR"
     gene            18403..19710
                     /gene="ynjE"
                     /locus_tag="ACHK07_001686"
     CDS             18403..19710
                     /gene="ynjE"
                     /locus_tag="ACHK07_001686"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO8102031.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVNTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TEKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19719..20339)
                     /gene="ynjF"
                     /locus_tag="ACHK07_001687"
     CDS             complement(19719..20339)
                     /gene="ynjF"
                     /locus_tag="ACHK07_001687"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8102032.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20426..20833
                     /gene="nudG"
                     /locus_tag="ACHK07_001688"
     CDS             20426..20833
                     /gene="nudG"
                     /locus_tag="ACHK07_001688"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO8102033.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20799..21067)
                     /gene="ynjH"
                     /locus_tag="ACHK07_001689"
                     /pseudo
     CDS             complement(20799..21067)
                     /gene="ynjH"
                     /locus_tag="ACHK07_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
     gene            21303..22646
                     /gene="gdhA"
                     /locus_tag="ACHK07_001690"
     CDS             21303..22646
                     /gene="gdhA"
                     /locus_tag="ACHK07_001690"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO8102034.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGKQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22763..23803)
                     /gene="ynjI"
                     /locus_tag="ACHK07_001691"
     CDS             complement(22763..23803)
                     /gene="ynjI"
                     /locus_tag="ACHK07_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8102035.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLITMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23930..25891)
                     /gene="topB"
                     /locus_tag="ACHK07_001692"
     CDS             complement(23930..25891)
                     /gene="topB"
                     /locus_tag="ACHK07_001692"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8102036.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFSPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25896..26939)
                     /gene="selD"
                     /locus_tag="ACHK07_001693"
     CDS             complement(25896..26939)
                     /gene="selD"
                     /locus_tag="ACHK07_001693"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO8102037.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(27056..27607)
                     /gene="ydjA"
                     /locus_tag="ACHK07_001694"
     CDS             complement(27056..27607)
                     /gene="ydjA"
                     /locus_tag="ACHK07_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO8102038.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQELSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27768..29624
                     /gene="sppA"
                     /locus_tag="ACHK07_001695"
     CDS             27768..29624
                     /gene="sppA"
                     /locus_tag="ACHK07_001695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO8102039.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GPIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29740..30450
                     /locus_tag="ACHK07_001696"
     CDS             29740..30450
                     /locus_tag="ACHK07_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO8102040.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEDAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            30582..31598
                     /gene="ansA"
                     /locus_tag="ACHK07_001697"
     CDS             30582..31598
                     /gene="ansA"
                     /locus_tag="ACHK07_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO8102041.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            31609..32250
                     /gene="pncA"
                     /locus_tag="ACHK07_001698"
     CDS             31609..32250
                     /gene="pncA"
                     /locus_tag="ACHK07_001698"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO8102042.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(32343..33701)
                     /gene="ydjE"
                     /locus_tag="ACHK07_001699"
     CDS             complement(32343..33701)
                     /gene="ydjE"
                     /locus_tag="ACHK07_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8102043.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVNVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSIFMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     ALGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(33819..34577)
                     /gene="ydjF"
                     /locus_tag="ACHK07_001700"
     CDS             complement(33819..34577)
                     /gene="ydjF"
                     /locus_tag="ACHK07_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8102044.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(34714..35694)
                     /gene="ydjG"
                     /locus_tag="ACHK07_001701"
     CDS             complement(34714..35694)
                     /gene="ydjG"
                     /locus_tag="ACHK07_001701"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO8102045.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGALDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(35704..36651)
                     /gene="ydjH"
                     /locus_tag="ACHK07_001702"
     CDS             complement(35704..36651)
                     /gene="ydjH"
                     /locus_tag="ACHK07_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8102046.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(36656..37492)
                     /gene="ydjI"
                     /locus_tag="ACHK07_001703"
     CDS             complement(36656..37492)
                     /gene="ydjI"
                     /locus_tag="ACHK07_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO8102047.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(37513..38556)
                     /gene="ydjJ"
                     /locus_tag="ACHK07_001704"
     CDS             complement(37513..38556)
                     /gene="ydjJ"
                     /locus_tag="ACHK07_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO8102048.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(38573..39952)
                     /gene="ydjK"
                     /locus_tag="ACHK07_001705"
     CDS             complement(38573..39952)
                     /gene="ydjK"
                     /locus_tag="ACHK07_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8102049.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIHQTGKPLPPVVIADDGKTPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(39979..41055)
                     /gene="ydjL"
                     /locus_tag="ACHK07_001706"
     CDS             complement(39979..41055)
                     /gene="ydjL"
                     /locus_tag="ACHK07_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8102050.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(41425..41697)
                     /gene="yeaC"
                     /locus_tag="ACHK07_001707"
     CDS             complement(41425..41697)
                     /gene="yeaC"
                     /locus_tag="ACHK07_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO8102051.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(41739..42152)
                     /gene="msrB"
                     /locus_tag="ACHK07_001708"
     CDS             complement(41739..42152)
                     /gene="msrB"
                     /locus_tag="ACHK07_001708"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO8102052.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            42494..43489
                     /gene="gapA"
                     /locus_tag="ACHK07_001709"
     CDS             42494..43489
                     /gene="gapA"
                     /locus_tag="ACHK07_001709"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8102053.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            43573..44457
                     /gene="yeaD"
                     /locus_tag="ACHK07_001710"
     CDS             43573..44457
                     /gene="yeaD"
                     /locus_tag="ACHK07_001710"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO8102054.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(44508..45362)
                     /gene="yeaE"
                     /locus_tag="ACHK07_001711"
     CDS             complement(44508..45362)
                     /gene="yeaE"
                     /locus_tag="ACHK07_001711"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO8102055.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLTGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(45452..46198)
                     /gene="mipA"
                     /locus_tag="ACHK07_001712"
     CDS             complement(45452..46198)
                     /gene="mipA"
                     /locus_tag="ACHK07_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO8102056.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            46634..48568
                     /gene="yeaG"
                     /locus_tag="ACHK07_001713"
     CDS             46634..48568
                     /gene="yeaG"
                     /locus_tag="ACHK07_001713"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO8102057.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            48681..49964
                     /gene="yeaH"
                     /locus_tag="ACHK07_001714"
     CDS             48681..49964
                     /gene="yeaH"
                     /locus_tag="ACHK07_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO8102058.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            50111..>50381
                     /locus_tag="ACHK07_001715"
     CDS             50111..>50381
                     /locus_tag="ACHK07_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8102059.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNY"
BASE COUNT        12626 a        13080 c        12187 g        12488 t
ORIGIN      
        1 gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc gaataaagcc
       61 atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag ggaggagtac
      121 cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaaaaac cgcaatttaa
      181 atattgcggt attgatttat gaaataactc tttgacggga aaatttaaaa taaattaatt
      241 tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac catcgacttt
      301 gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac gctggatttc
      361 gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct gacctttttc
      421 accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt tttctgcctg
      481 tgcgcgcatt tttgatacca gtaaagaggt agacatgccc gcagaacaaa acagataaat
      541 gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag acactccgca
      601 agccttaacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact ttttttgtgg
      661 ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt ataaattgtc
      721 tctccttgac ctgcggttat gaccctcttc acatttcggg caaataattc gcgaagataa
      781 ttaagaactc agaccaaaaa aaaccggcgc aatggccggt ttccgttgtt actcaagctt
      841 tcagacgaag tgattacttc gcagcctgtg gatcagtgtc gtattcagca caggtctgat
      901 aaccggagtt gatgacatgt ccggtatcat ctaacgcgac aaagtaggtt tctgctttac
      961 catcacgttg acccaggatg taggtctggc aagtaccacg agcatggatc atgctcactt
     1021 cagacgaagg tttacccgca atttgtgcaa cctgcgcccg gctcatgcct tttttgacgt
     1081 ctttcaccac aggctgtaca aactggtctt tggtacgatc ataagccgta caacccgcca
     1141 gcatggttaa taccgccgct gcactcagaa ttcctgccat attcttgttc atattccgtc
     1201 ctcttgttta tcagcgtgtt agataagcct ggaatacatt gggcgctttt tcaagcccgt
     1261 gaacgaaacg gctccgcttt cagaggattc ctgtatgacg ttttaaccac cattcagccc
     1321 gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa ggaagttttg tcttttctgt
     1381 ctggaggggt tcaatgacat tgcaacaaca aataataaag gcgctgggcg caaaaccgca
     1441 gattaatgcc gaagaggaaa ttcgtcgtag tgtcgatttt ctgaaaagct acctgcgaac
     1501 ttatccgttc attaaatcac tggtgctcgg gatcagcggc ggtcaggact ccacgcttgc
     1561 cggtaagctg tgccagatgg cgattaatga gctgcgccag gaaaccggca acgaatcact
     1621 gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc gacgaacaag attgccagga
     1681 tgccattgcc tttattcaac cggatcgcgt attaaccgtt aatatcaagg gcgcggtatt
     1741 ggctagcgag caggcattgc gggaagcagg cattgaactg agcgattttg tccgtggcaa
     1801 tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt gcgggtatga ccagcggtgt
     1861 cgtggtgggc accgatcatg cagcagaagc cattaccgga ttcttcacta aatatggtga
     1921 cggcggtaca gacattaatc cgctgtatcg tctcaacaaa cgtcagggta aacagttact
     1981 ggcggcatta ggttgcccgg aacaccttta taagaaagcg ccaacggccg atctggaaga
     2041 tgatcgccct tctctgccag atgaagtggc actcggcgtg acctatgaca atatcgacga
     2101 ctatctggaa gggaaaaacg tacctcaaca ggtcgccaga acaatagaga actggtatct
     2161 gaaaaccgaa cataaacgcc gtccgccaat taccgttttc gatgatttct ggaaaaagta
     2221 ataatttgca cctattggat tgtgcgaaaa agagtaattt gttcacgccg gatgcggcgt
     2281 gaacgcctta ttcgacctac aaaactatgc aaattcaata cattgcagga gtcgaatggg
     2341 cctgacaggc gtagcacgtc agacggtgta acctttgtca tcaacccgcc tcttttttaa
     2401 tcgcttcacg cctgttacac tggatagata accagccttc ggagtcaaca gtggtacggc
     2461 gtttaacttc tccgcggctc gaatttgaag ctgcggcaat ttatgaatat cccgaacatt
     2521 tacgttcatt ccttaatgac ttacccaccc gaccaggggt gtatctgttt catggcgaaa
     2581 gtgacaccat gccgctctat atcggcaaaa gcgttaatat ccgcagtcgg gtgctttctc
     2641 atttacgcac cccggatgaa gccgccatgc tacggcaatc ccggcgaatt agctggatct
     2701 gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa gaacaacagc
     2761 cgctgtttaa taaacggttg cgccgcaatc gccagctctg tgccctgcaa ttaaatgaaa
     2821 agcgcgtcga tgtggtgtat gccaaagagg tggatttttc acaagccccc aacctgtttg
     2881 gcctgtttgc caataggcgc gcagctttgc aagcattgca gaccatcgct gatgaacaaa
     2941 aactttgtta tggcctgctg ggactggaac cgttaagtcg cggtcgtgca tgttttcgtt
     3001 cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga gagccatgag gaacatgcgc
     3061 tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg ttggccttgg caaggtgcgg
     3121 tggcactgaa agaacagcac ccggaaatga ctcaatatca tattattcaa aactggctgt
     3181 ggctgggggc ggttaattcg cttgaagaag cgacaacgtt aattcggaca cccgccgggt
     3241 ttgatcacga cggttataaa attctttgta agccgctgct ttccggtaac tatgaaatta
     3301 ctgaacttga tccggcgaat gaccagcgag ccagttgatt tcactgaaca acagtttacc
     3361 ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac cagcacgcgc cctggtcggt
     3421 agtgagcagt ttgtcgccca gttgccacgc cccgttaatc acaaacacca ccccgccgcg
     3481 tgagccaaag gtagtaaacg tacgttcggc aattctgact ttcgccttac agacatccag
     3541 gcgtgtcatg atgttgaaat ccatcgacat ctgcccttcc gtcagtttcg cttttaccac
     3601 ctggtccgcc gcaaaggcaa aaggttgtaa cggttttaaa gtatggttaa agcggtctgt
     3661 gctttcaaga aacatctcgc cgccttccag caacgtcact atcctttcca taccgggaaa
     3721 caaagaaaac tccccattag ccgcgatgga tgcaatgctg gcacgccagt aaaaatcacg
     3781 ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg gcagcgtttc gccacagatt
     3841 caccgacatt ttacgcatat caaagtattc catactcgct cccggcccgt tcttttcatt
     3901 gttatcgttc aggtaaccgt ggatggttat cggcaactcg ggtgatttac gttagtggtg
     3961 atcaggcttt ttttacaact gtttgagaag agaaaagaaa accgccgatc ctgtccaccg
     4021 cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg aaagattatt
     4081 cagcagttgc aggcatttta ccttttgccg ctggacgttc tgtcagacgc ttctcaaaat
     4141 tagcattaaa ttgctttttc tgttccggcg tcaggatgtt gtaaattttg ttctgggttt
     4201 ccatgtgcgc cagcatgtta gctttgcgct gttcttccat ttttgcgatc tgcgcttcag
     4261 cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg cattgcgcgg cgttcttcca
     4321 gcggcggacg tttcatctgg tcacgctggc ctttcatgat ttcgcggatc tgctgtttct
     4381 gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg atgcggaccg aacttgcctt
     4441 tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt agtggtgtct gcggcatggg
     4501 ccaggttagc cgcgccaaga gccagggtag aggcaacaaa cagtgcagtt aatttacgca
     4561 tattctatat ccttcctttc agttatttat tacggctttc ttaagtagcg tgccgtgttg
     4621 acgagattaa ctttactgac tttagcgtca attaatcaga gcaacggtaa aacaatgaaa
     4681 gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag aaaatttgat ggagagtgat
     4741 gagcgaatat tacaacacga tgattttgca gagattatga agaactatac cggatgactg
     4801 gtgataaata aagcaaataa ccaggattaa tctgtattaa tttataagaa agcaacttaa
     4861 tacccgcaga atgatttctg cgggtaagta ttagcttatt ttttcgagca ttaatcccgc
     4921 gcgtaacccc aacgctacca acggattagg gaataacaca tactctacat catgggttac
     4981 ggtaaaacgt tcctcaccgt cctgcgccag caacgttcct ttcttaaacg gcataaaatt
     5041 cagcgtgtca cttgccatat gcatttcgaa ggacggcgag tggcgagtaa tttgcgaaac
     5101 cacccgataa cggagcggcg gtgttctcac gataccgaca ctctcaccag atagcagcgc
     5161 agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg ttttgcccaa agggcaacgc
     5221 tttgccaagt tccagcgtac aggccagcgc gccaaaatgt ctggcgctga aatgggtaaa
     5281 cgtaccacca ggctcctgat ggaacaccag cgcctccagc cccgccgcac ccagccacgt
     5341 cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat acaccgaact gcggatgcaa
     5401 ggagccacga attgcggtat gtagatcaag gtgccagcgc acagattctt tgccctggtc
     5461 ataaaaatct tccaggcact gttccagttc gcgcgcccga caggtttctc cgctttcagc
     5521 aaacagctgc caacgaccgc caaacattcg gttcatatcg ctatggcaat aacgtttccc
     5581 ttgcttcagc gcaggaggat tcccgaggat caccagcaac cgccaacgta acgggatctc
     5641 gccgtgagat atcgcgccaa gcaacgcgtc cagcatttcc accggagccg tttcattacc
     5701 gtggatcccc gctgaaatca ccagtgcgcc ttgcggtggc gttaatggcg tcagttccag
     5761 cacaccatcg cccagccagc gccagcgaac accgttgatt tctcgctcgg tgataaccgg
     5821 ttttttactc gttaaggtca gagcaagaaa attatccatt gccccctccc tcgcgctgga
     5881 acggataaac cgaaccgaga ttcagtaatt ggctcaatac atccagtgct tcccgccctt
     5941 cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag acgatcgcgg tagtaacgat
     6001 ccacccagtc attgagcgca ttaaacaagg tatcgttcat catcaccgcc ggattcaccg
     6061 cccggcgttc ttcttgtgtc aataccaccc gcaaccgcag gcacgccggg ccaccgccat
     6121 tcgccatgct ttcacgtaaa tcaaagactt ttagttcgct gatcgggttg tcagcggcaa
     6181 ggagttcatt gagataacac catactccgg cgtgttcccg acactcctgt ggcagcacca
     6241 acatcatgga accatcatcg cggctcaaca gttggctgtt aaacagatat gtagacaccg
     6301 catccgacac ggaaacctga gttgccggaa cttctatcgc cataaaaccg ttcacccgcg
     6361 cacgcaggtt tgccagtaac tgtgactggc gagcgaacgc ctgttggtgg caaaacagca
     6421 cctggcggtt actcacggca atcacgtcat tatgaaaaac gccctggtcg ataacgtccg
     6481 ggttttgctg ggcgaaaatc acctgttggg gattcacctg attcagcctt gccacagcct
     6541 cgctggcttc gcgagtctgt cgcgccggat aacgggaagg ccgggtatca ttgccttttt
     6601 ctcgcccgta gacaaaaagt tgcatacccg gttcaccgta atgaccgccg agacgattgt
     6661 ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg caacgccgaa tggacgctaa
     6721 atttctcttc gtcgttaaaa atagctttta acagcgattc agtgacgggc gcttccagcg
     6781 aacggtgaaa tttattgttc aggttggcaa cggtgagatg cactttgcca tccagcgtat
     6841 cggcagatgg cgcgatcgtt gccgcattgg ctacccacat tggcgaagcg gagctgacgc
     6901 tggaaagcca gtgcggtgcc tggcgtgcaa ctttttccag tacctgctca tcgctaccac
     6961 tgaatcccaa ctgacgcagc accggaataa acggacgctc gtgcggcggg atcacggcct
     7021 gggggaatcc cgcatcggca agggttttca ttttcagtaa gccctgtttc gccgccagtc
     7081 gcgggttaga cacctgaaaa cggtgacggg tagaggcttc attaccaaac gacaggccag
     7141 cgtaattgtg tgtcagccct accagcccgt cgaaattaac ttcccaagcg ttcatcgcac
     7201 cacctcatcg gaaaaatcca gtccggggtt aagcgtggcg ggtaacgtta acgagtccga
     7261 ctccaggctc gccataggcc atgcgcagta atctgcggca taccaggcgc tggggcgatg
     7321 gttaccggat gcaccaatgc cgccgaatgg cgcggtactg gcagcaccag taagcggttt
     7381 gttccagtta acaatccccg cccgcgcttc cagcaacagt tgttcgaact tttcccgctc
     7441 gggggaaacc agaccgcaag agaggccgaa gcgagtgtta ttcgccatcc tgatcgcctc
     7501 atcgaaattg tcataacgcc agacgcgcaa gagtggccca aacacttctt catccggcac
     7561 gccagcaacg cacgtcattt caatgatccc cggcgtcagc aacgatgttc cggcttgcag
     7621 caagtgcggc gcaagcaggg ttcgtccgcc catcgcttcc agttgctgcc aggcagtaac
     7681 cacctgctgt gcggcctgtt cagaaatcag cccgccaata aacggctgcg gttcgtcatc
     7741 ccagttgccc ggcgttaatc gctggctgac ggcaaccaga cgagcaagaa acgcatcgcc
     7801 ctgcgccccg cttttcagca ataaacggcg ggcgcaggtg cagcgttgac cggctgtgac
     7861 aaacgccgac tgaatggtca gatggacagc ggcgtcgata tccgccacct catcgataat
     7921 tagcggatta ttaccgccca tctcaagggc gagaattttc tccggctgac cggagagctg
     7981 gcgatgcagc tggtagccgg tattggcgct accggtaaac agcaagccgt cgagatcctc
     8041 cagcgcactc agcgcctgac ccgtttcacg cccgccctgc accaggttca gcacgcccgg
     8101 cggcaagcca gcctgctgcc ataaacgcat taccgcttcg ccactccacg gtgtcagttc
     8161 gctgggttta aagataatgg tgttacctgc cagcaatgcc ggaacgatat gtccgttcgg
     8221 caaatgacca gggaaattat acggcccaaa caccgccagc acgccgtgcg ggcgatgtcg
     8281 caggctcgcc gcgccgtccg gcatttcact acgctgctcg ccggtacgaa cgtgatacgc
     8341 cttaattgaa atcgcgattt tattgatcat cgccgtcact tcggttgccg cttcccagcg
     8401 cggcttgccg gtttctcggg caatgattgc ggttaattcg gctttattgc tttccagcaa
     8461 tccggcaaag cgttcgacaa ctgcctgacg ttcagcaaat gagagccgcg cccagcgcgg
     8521 aaacaccgca cgggctgcgc gacaagcctg cccgacctga gcggcatcgg catcattgcc
     8581 ttgccataac acctcgcccg ataccggatt acgcttcaca cgcgatgcac cctggcccgt
     8641 tatccagtca ccgttaatcc ataaagtcat gctgttttct cctctgcgca caggcgcacc
     8701 agacgaacgc gatcccctgc gtggcatttg agggcatcca gttgtgcggc ggttaaaatc
     8761 aaacgctcgg ttgccggatc ggtacgcacc agcaccacgc ggaaatggtg ataattttca
     8821 ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct gcccttctgc cacttccacc
     8881 agccgacttt tacggatggc gcgcacgcgg tcgatgtcac actcaagcgt cggcccaccg
     8941 tcgaagatgt cgatataatt acggtagcga aaaccttctt tctccagcac cgcgcgggca
     9001 ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg cctcctggga taaaaagtgc
     9061 gtatagatcg gatgtttcgg catcagttct gcaataaatg ccttttgccc ggtgccgcag
     9121 agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt taccgaggct ttgccagaac
     9181 ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag caaccacttt gtcattaaac
     9241 ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg acagcaaata gccgttgccc
     9301 tctttgcgcc agtccgggtc gagaaacagc gtgcacagct cgctgctgcc ggtgtgatcg
     9361 ttactgagaa acagcgttgg cagcgcgtta tagacattca gctctttcga agcgtgaacc
     9421 aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc caactgccac ctcaatggcg
     9481 caaatccccg ccacaatgcc tgtctcgcta tcttccagta caaacacata accctgctca
     9541 cttttgggca gttcaccttg ccaggttttg attgcccttt cgatacgcgc cgaaagcgtg
     9601 gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt tgctggcaag ctgcatcagc
     9661 gccgagacat ctgagcgttc aacgggacgg atgaccatca tgatgaacct ccgctcacaa
     9721 agcgttcaca ggccgcagca aagcgatcca gtccggtcgt cacctcttct tcgctgacat
     9781 tgagcgcagg cgcaaaacgc accacgttgc cacccgcaat cagcaccatc acgcccgctt
     9841 tcgccgcctc ctgagagatc tgtttcgctt gtccggcgta atcggcattc agtacacagc
     9901 caatcagcaa acctaagccg cgaacttcac tgaacaaacc atagcggtga ttaataatat
     9961 taagacgctc aacaaaccag tcgtgacgtt gcttaacccc gttaagcatc tctggcgtat
    10021 tgatgagctc cagcactttg cctgccaccg ccgaggccag cggattgccg ccataggtgg
    10081 tgccatgagt gccaacggtc atcacgctgg cgcactcttc ggttgctaac aacgcaccga
    10141 ccgggaaacc gccgcccagc gctttggcgg tagttaacag atcaggcgtc acgccgtagt
    10201 gcatataggc atacagttcc ccggtgcgcc cgacgccggt ttgtacttca tcaaaaatca
    10261 acagcgcatt gtggcgatca cacaattcac gcagaccttg taaaaatgcg ttgctggctg
    10321 gcaccacacc gccttcgccc tggatgggtt cgacaatcac cgcacaggta gagtcgtcaa
    10381 tcagcgcgct ggcagagtta atatcgttat atgcagcatg acggatatct ggtggcagtg
    10441 gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact gacagtaaac agcgtgcgac
    10501 catgaaacgc atttttaaac gccacgatac cgctcttatg gctgccgtag cggtcgtgag
    10561 cgaatttacg cgccagtttt agcgccgctt cgttggcttc cgcgccagag ttacaaaaga
    10621 agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc cagtcgcagt accggttcgt
    10681 tggtgtaacc gttgccggta tgccagaact tactcgcctg ttcgttcagc gcctcgcgca
    10741 gttccgggtg cgcatgtccc agcgcgttca ccgcaatgcc acccgcgaag tcgatatact
    10801 ctttcccctg ctgatcccac aagcgcgaac cttcgccacg taccggtata aacggtgccg
    10861 gagcgtaaac aggtatcatc cattcatcaa agttttcacg cgtaattggc tgagacatag
    10921 cgacctctac agtaaataat tcgttattta tatgttaata ataagtaata tttgcgctgt
    10981 aaatgtagat tgcagggttc gtgccagcca gtgataaaag tgcataaacc gcggaggcta
    11041 actggaaatc aaggagttat aaccaaacca tatgcattta aagtgcatat aaagtgaata
    11101 cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa tgttatgcag aagtaaaata
    11161 taattctgga attgtgatca ttgacgaaat ttactggaaa ttactgcgcc attctgacgc
    11221 aacgcgcacc aaaagcgggc attttttgcg ccatcgttga catcattaac aaccatcgat
    11281 caaatcactt aacaacaggc ggtaagcaaa gcgaaattct gctaccatcc acgcactctt
    11341 tatctgaata aatggcagcg actatgaaat ttgtctcttt taatatcaac ggcctgcgcg
    11401 ccagacctca ccagcttgaa gccatcgtcg aaaagcacca accggatgtg attggcctgc
    11461 aggagacaaa agttcatgac gatatgtttc ccctcgaaga ggtggcgaag ctcggctaca
    11521 acgtgtttta tcacgggcag aaaggccatt atggcgtggc gctgctgacc aaagagacgc
    11581 cgattgccgt gcgtcgcggc tttcccggtg acgacgaaga ggcgcaacgg cggattatta
    11641 tggcggaaat cccctcaccg ctgggtaatg tcaccgtgat caacggttac ttcccgcagg
    11701 gtgaaagccg cgaccatccg ataaaattcc cggcaaaagc gcagttttat cagaacctgc
    11761 aaaactacct ggaaaccgaa ctcaagcgtg aaaatccggt gctgattatg ggcgatatga
    11821 atatcagtcc tggcgacctg gatattggca ttggcgaaga gaaccgtaag cgctggctgc
    11881 gtaccggtaa atgctctttc ctgccggaag agcgcgaatg gatggacagg ctgatgagct
    11941 gggggttggt cgataccttc cgccatgcga atccgcaaac agcagatcgt ttctcatggt
    12001 ttgattaccg ctcaaaaggt tttgacgata accgtggtct gcgcatcgac ctgctgctcg
    12061 ccagccaacc gctggcagaa tgttgcgtag aaaccggcat cgactatgaa atccgcagca
    12121 tggaaaaacc gtccgatcac gcccccgtct gggcgacctt ccgccgctaa tttagcagct
    12181 ctcctggctc aaactgggtc aggagaatta accttgagaa aaatcaacaa actgtcagta
    12241 atgatttgtt gcctgccgtc ctttgttata ccgtcactgc gtttttagtt gtctgaccac
    12301 ttctctatta tcaagtttga tataggaaac tccacgatga acgctgagcg taaatttctg
    12361 tttgcctgtc ttatttttgc gctggtcatt tacgctatcc acgctttcgg tttattcgat
    12421 ctgctcaccg atttacccca cttacagaca ctcattcgcc agagcggatt gttcggttat
    12481 agcctctata ttctgttatt catcattgcc accctctttc tgttaccagg aagcatactg
    12541 gtgatcgcgg gaggaatagt ttttggcccg ctcttaggga cactactctc attaattgcc
    12601 gctacgctgg cctcctcgtg ctcattcctg ctggcgcgct ggatgggacg tgatttactg
    12661 ctgaaatacg tgggccatag ccataccttc aaggctatag aaaaaggcat tgcgcgtaac
    12721 ggcatcgatt ttcttattct gacccgctta attccgctgt ttccgtacaa tattcaaaat
    12781 tacgcttacg gattaaccgc tattgctttc tggccctata cccttatttc ggcactcacg
    12841 accctgcccg gaatcgtaat ttataccgtg atggcaagcg atcttgccaa tgaaggcatt
    12901 acgctgcgct ttattttaca actctgtctg gcgggcctgg cgctgtttat tctcgtccag
    12961 ctcgccaaac tctacgcccg acacaaacat gtggatctgt ctgcttcgcg ccgcaaccca
    13021 cttactcacc caaaaaatga aggatagaac gatgttgcaa cattattcag tgtcatggaa
    13081 aaaaggactg actgcactct gcttactggc tgttgctggg cttagcggct gcgatcaaaa
    13141 agagaatgcg gcggcaaaag tggaatacga cggactttcg aacagccaac cgctgcgcgt
    13201 cgatgccaat aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt tcctcaccga
    13261 cgacactcgt cacggtattg tgtttaaaga tggctccaac ggacataaat cgctgtttat
    13321 gggttatgcg accccgaaag cattttatga agccctgaaa gaggcaggtg gtacaccggg
    13381 cgaaaacatg acgatggata ataaagaaac gactcatgtc acaggcagca aactggatat
    13441 ttcggtcaac tggcaagggg cggcaaaaac gtattccttc gatgaagtga ttgttgacag
    13501 taatggcaag aaactggaca tgcgctttgg cggtaattta acggcagcag aagagaagaa
    13561 aacgggttgc ctggtgtgtc tggatagctg cccggtcggc atcgtcagca atgcaacata
    13621 cacttatggt gcggttgaaa aacgaggtga agttaaattc aaaggcaatg cctcggttct
    13681 cccggcggat aacacgctgg caacggttat ctttaaaatc accgaataaa gccaggataa
    13741 aggatgatga agatgcaatc gcgaaaaatc tggtactacc gtataaccct catcatcctg
    13801 ttgttcgcta tgctgctggc atgggcgctg cttcccggcg tccatgagtt tatcaatcgc
    13861 agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag aacgctttat tcagtcttac
    13921 ggcgcactgg cggcagttgt ctcgttcttg ttgatgattt tacaggccat tgctgcaccg
    13981 ctacctgcgt ttttgatcac ctttgccaat gcgtcgctat ttggcgcgtt ctggggcggc
    14041 ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt gcttttttat cgccagagtg
    14101 atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg tacttgacag tatggacggc
    14161 tttttcactc gctacggcaa acacactatc ctggtctgtc ggttattgcc ttttgtccct
    14221 ttcgatccaa tcagctatgc tgccggtttg acttcaatac gttttcgctc gttttttatc
    14281 gccaccgggc ttggtcaatt accggcgact attgtttatt cctgggcggg cagcatgtta
    14341 acagggggta ccttctggtt tgtcaccgga ctgtttattc tgtttgccct gaccgtggtg
    14401 atttttatgg cgaagaaaat atggcttgag cgccagaaga ggaatgcctg atgggtttac
    14461 cgccgcttag caaaattcct ttcattttac gtccgcaggc gtggctgcat cgtcgccatt
    14521 acggcgaggt gctaagcccc attcgctggt gggggcggat cccgtttatc ttttatctgg
    14581 tgtcgatgtt tgttggctgg ctggagcgca aacgctcacc gctcgatccg gtagtacgat
    14641 cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga gttttgtgtg gatatcacca
    14701 gtatgaaagt cgccgagcgc accggcagca gcgataaact gctggcagtg gctgactggc
    14761 ggcaaagccc gctctttagc gatgaagaac ggctggcgct ggagtacgcc gaagccgcaa
    14821 gcgtaacgcc gccaacggtc gatgatgccc tgcgtacccg actggctgcg cattttgacg
    14881 ctcaggcgct caccgaactg acggcattga tcggcctgca aaatctgtca gcccgtttta
    14941 attctgccat ggacattccc gctcaggggc tgtgccgtat tccggaaaaa cgttcttaag
    15001 gagagatgat gcgccattgt gggtggttgc tgggattgtt atcgctgttt tctctggcaa
    15061 cacatgccag tgactggcaa gaaattaaaa atgaggccaa agggcaaact gtctggttta
    15121 acgcctgggg cggcgatacc gcaattaacc gctatctcga ctgggttagc ggcgagatga
    15181 aaacccatta cgctataaac ctgaagattg ttcgtctggc ggatgccgca gacgcggtga
    15241 agcgcattca gaccgaagcc gcagccggac gtaaaacggg cggctcggtg gatctactct
    15301 gggtgaacgg cgaaaacttc cgcaccttaa aagaggccaa tttactgcaa accgactggg
    15361 cagagactct gcccaactgg cgctatgtcg acacacagct gccggtgcag gaagattttt
    15421 cagtgcctac agaaggggct gaatcgccct gggggggcgc acaactgaca tttatcgccc
    15481 gccgcgatgt tccgccacag ccaccacaaa cgccgcaagc cttactggag tttgctaaag
    15541 ccaatcccgg cacggttacc tatccgcgcc caccggactt taccggcacg gcgtttcttg
    15601 aacagttgct gattatgctg acggccgatc ccgccgcatt aaaagaagcg ccggacgatg
    15661 cgactttcgc ccgtgtcact gctcccttgt ggcaatatct tgatgcgctg catccgtatt
    15721 tgtggcgcga aggaaaggat ttcccgcctt cgcccgcgcg gatggatgct ctgctgaaat
    15781 ccggcacatt gcgcctgtcg ctgaccttta accccgcgca tgcgcagcaa aaaatcgcca
    15841 gcggcgattt gcctgcaagc agttacagtt ttggctttcg cgaggggatg attggcaacg
    15901 tgcatttcgt caccattcct gccaacgcga atgccagtgc tgcggcgaag gtagttgcca
    15961 atttcttgct ctcacccgat gcgcaattgc gtaaagcaga tcccgctgtc tggggcgatc
    16021 cttctgttct cgatccgcaa aaactgcctg atgggcagcg cgaaacattg caatcaagaa
    16081 tgccgcaaga tctgccgcca gtactggctg aaccgcacgc aggttgggta aatgcgctgg
    16141 aacaagaatg gctacaccgt tacggtacgc attaatcttt ttgctgtggg cgatggtggc
    16201 ggtgatttat gcaccgctga tcccggcagc tctcacgctg atatcgcctg ctttgtcgtt
    16261 gacacactgg caggcgttat ttgccgatcc acagttaccg caggcattac tggcaacgct
    16321 ggtgtcgaca accatcgcgg cggtcggggc gttgttgatt gccctgctga tgattgtggc
    16381 gctgtggcct gggccgaaat ggcagcgtat gtgcacccgt ctgccgtggc tgctcgccat
    16441 tccccatgtg gcttttgcca ccagcgccct tctgctcttt gctgacggag ggctgcttta
    16501 tgactatttc ccgtatttca ctccgccaat ggacaaattg ggtattgggc tgggcttcac
    16561 gctggcggtg aaagaaagcg catttctgct gtggatctta gcagcggtat tgagtgaaaa
    16621 acggctttta cagcaagtca tcgtgctgga ttcgctgggc tacagccgct ggcaatgcct
    16681 gaactggctg ctgttgccct ccgtcgcgcc tgcgctggca atggcgatgc tggcgattgt
    16741 tgcctggtcg ctgtcggtcg tggatgtggc gattattctt gggccaggta atccaccgac
    16801 gctggcagta attagctggc agtggttaac tcagggcgac gccgatcaac aaacgaaagg
    16861 cgcactcgcc agcctgctgc tgatgttgtt actcgccgcc tacgttttgc tgggctatct
    16921 gctatggtgt acctggcggc gtactattcc ccgcgtagat ggcgttcgta agcctgccac
    16981 gcctttattg ccgggcaata cgctagcgag ttttttaccc ttgaccggtg tgctgtgtgt
    17041 aattctgctg gcgatcctcg cggatcagtc gacgatcaat agtgaagcgc tcatcaacag
    17101 cctgacaatg gggctggtgg cgacattcat cgctttgctc ctgttactgc tgtggctgga
    17161 atgggggcca cagcgtcgcc agttgtggct atggttgccc attttattac cggcactgcc
    17221 actggtggcg ggccagtaca cgctggcgct atggctgaac ctggatagga gctggacggc
    17281 ggtggtctgg gggcatctgc tgtgggtaat tccgtggatg ctgtttatcc tgcaacccgc
    17341 ctggcagcgc attgattcac ggttaatttt gattgcacaa acactgggct ggtcgcgggc
    17401 caaaatcttc ttttacgtga aatgcccact catgttgcgc cctacgctga ttgccttcgc
    17461 ggtgggattt tcagtcagta ttgcgcagta tatgccgaca ctgtggctgg gcgcggggcg
    17521 ttttccgacg ctcaccactg aggcggtggc attaagcagc ggcggcagca acggtattct
    17581 cgccgcccag gctttatggc aactgctatt accgcttatt atttttgccc tgaccgcgtt
    17641 agtcgcaaaa tgggtaggtt atgtcagaca aggactccgc taatgctctg cgtgaaaaat
    17701 gtttcgctac gtttaccaga aagttgcttg ctgacaaacg ttaactttac ggtggataaa
    17761 ggtgacattg tcacgttaat ggggccgtct ggctgtggaa aatccactct gttttcatgg
    17821 atgattggtg cactgcccgg acagttttct tgtacaggtg agctatggct caatgagcaa
    17881 cggattgaca tgctacccac cgcacagcgt cagattggca ttctttttca ggatgcactg
    17941 ttatttgacc agttcagtgt cgggcaaaat ttacttctgg cgctaccggc gacacttaaa
    18001 gggaatgccc gacgtaatgc cgtgaatgat gcacttgagc gttcaggcct tgatggagct
    18061 ttccatcagg atcctgccac tttgtctggc ggtcagcgag cgcgcgttgc tctgcttcgc
    18121 gcccttctcg cccagccaaa agcgttactc ctggatgagc cattcagccg tcttgatgtg
    18181 gctctgcgcg ataattttcg ccagtgggtg ttcagcgaag ttcgcgccct ggcgatcccc
    18241 gtcgttcagg taacgcacga tttccaggat gttcctgctg atagttctgt tctggatatg
    18301 gcgcagtggt cagaaaatta caacaaactg cgataacgca aagtttttct caatgcgtca
    18361 gttcagaatg gcgcactcaa aactacaatg tcgggatttt cgatgaaacg tgtttctcaa
    18421 atgaccgcgc tggcaatggc tttagggctg gcttgcgctt cttcgtgggc cgctgaactg
    18481 gcgaagcctc ttacacttga ccagcttcaa caacaaaatg gcaaagcgat agatactcgc
    18541 cccagcgcgt tttataacgg ctggccacaa accttaaatg gcccttctgg tcatgaacct
    18601 gccgccttaa acctctctgc cagctggctt gacaaaatga gcaccgaaca gctaaacgcg
    18661 tggatcaagc aacataacct gaaagccgat gccccggtgg cgctgtacgg taatggcaaa
    18721 gatgtcgacg ccgtcaatac gcgactgcaa aaagcaggtt ttacgcatat ctccattctg
    18781 agtgacgcgc taagcgaacc ttcccgtcta caaaaactgc cgcactttga gcagctggtt
    18841 tatccgcaat ggctgcacga cctgcaacaa ggtaaagagg ttacggagaa acctgccgga
    18901 gactggaaag tcattgaagc ggcctggggc gctcctaagc tttaccttat cagccatatt
    18961 cccggtgctg actacatcga taccaacgaa gtggaaagcg aaccgctgtg gaacaaagtt
    19021 tctgatgaac aactaaaagc gatgctggca aaacacggca ttcgccatga caccacggtc
    19081 attctgtacg ggcgtgacgt atacgctgca gcgcgtgtgg cgcagattat gctttatgcc
    19141 ggcgtgaaag atgtgcgcct gctggatggc ggctggcaaa cctggtccga cgcgggtctg
    19201 cccgttgagc gcggtacgcc accgaaagtg aaagcggaac cggatttcgg cgtgaagatc
    19261 ccggcacaac cgcaactaat gcttgatatg gaacaggcgc gtggactgct gcatcgccag
    19321 gatgcatcgc tggtgagcat tcgttcgtgg ccagagttta tcggtacgac cagcggttac
    19381 agctatatta aaccaaaagg tgaaatagcc ggagcacgtt ggggacacgc tggtagcgac
    19441 tcgacgcata tggaagattt tcataacccg gatggcacca tgcgcagcgc cgatgatatt
    19501 acggctatgt ggaaagcatg gaatatcaga ccagatcagc aagtttcatt ctactgcggc
    19561 accggctggc gcgcgtccga aacctttatg tacgcacgag caatgggctg gaataacgtc
    19621 tccgtttacg acggcggctg gtacgaatgg agcagcgatc caaaaaatcc ggttgcaacc
    19681 ggtgaacgcg gcccggacag cagcaaataa cattgcaatt actgacgctg gagcgacttc
    19741 agcgtcagat acccactcca cacccgcgta aaggtcgtca tccagcacaa cgcaccaaat
    19801 acccacgcaa accacggaaa acaggtcgga aagaggcaac tcagaacaaa cagcaaaatc
    19861 gtctcgctgc cttcggttaa cccgcccaga taataaaacg atttatgcgc ataaccggga
    19921 ttatcaatct gatgtttcgc tgccagtgca gcaaaggcga gaaaactgct gcccgtaccg
    19981 ataaacgcaa acaacaacca gccgcctgcc agcgcatttt gctccggtgc agcaagaata
    20041 aaaccaaacg gcaccagcgc gtaaaagaga aaatccagcg agatatctaa aaacccgcct
    20101 gcatcggtta atcctctgcg ccgcgccagt gcgccatcca gaccatcaag cagacgattc
    20161 aacaaaatga cgactagcgc cgccagatac cagcccagag ccagaaacgg cagagccagc
    20221 acgccgatgg caaatcccac taatgttaaa ccatctggcg taatgcccgg tttatcaaga
    20281 acccgcacgc actgatgcaa caacggttta atccggggat gaagatggcg gtctagcaca
    20341 ggcactcctt aaatataaag cctttctgat tgagcaacag tgcggatatt atggcatttt
    20401 tcgcttatct gcccgtgtgt aatttatgaa aatgattgaa gttgttgccg ccatcattga
    20461 acgtgatggc aaaattttac tcgcgcaacg ccccgcccag agcgatcagg cgggattatg
    20521 ggagtttgcc ggtggtaaag tcgagctgga tgaaagccag cagcaggcgc tggtgcgtga
    20581 gttaaacgaa gaattgggca tcgaagcaac tgtgggtgag tatgttgcca gccatcagcg
    20641 agaagtttcg gggcggatta tccatcttca tgcctggcac gtacccgact tccacgggac
    20701 gttacaggca catgaacatc aggcgctggt ctggtgctca cctgaagagg cgctgcaata
    20761 tccgctggcc cctgctgaca ttccactatt agaggcattt atggctttac gcgctgccag
    20821 accagcggat tagtgttaag gggtcatcac gctggcattg cagcagtatt ccctcggctt
    20881 taattaccgc tccttcagaa taatttttat cctgataaac acagcactga gtacagggct
    20941 gcgctgactg cccgcctgaa ctgaacactt ctggcggtac gtttacctcc acgtccggac
    21001 gataatgcgg gttagccagt gcaattaatg gaaatgctaa tactacggcg aacaatgctc
    21061 gactcacagg gaactcctta acgttattgt ctctgctact gataacggta gccgggtggc
    21121 aaaactttag cgtctgagtt atcgcatttg gttatgagat tactctcgtt attaatttgc
    21181 tttcctgggt catttttttc ttgcttaccg tcacattctt gatggtatag tcgaaaactg
    21241 caaaagcaca tgacataaac aacataagca caatcgtatt aatatataag ggttttatat
    21301 ctatggatca gacatattct ctggagtcat tcctcaacca tgtccaaaag cgcgacccga
    21361 atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac cacactctgg ccttttcttg
    21421 aacaaaatcc aaaatatcgc cagatgtcat tactggagcg tctggttgaa ccggagcgcg
    21481 tgatccagtt tcgcgtggta tgggttgatg atcgcaacca ggtacaggtc aaccgtgcat
    21541 ggcgtgtgca gttcagctct gccatcggcc cgtacaaagg cggtatgcgc ttccatccgt
    21601 cagttaacct ctccattctc aaattcctcg gcttcgaaca aaccttcaaa aatgccctga
    21661 ctactctgcc gatgggcgga ggtaaaggcg gcagcgattt cgacccgaaa gggaaaagcg
    21721 aaggcgaagt gatgcgtttc tgccaggcgc tgatgactga actgtatcgc cacctgggcg
    21781 cggataccga cgttccggca ggtgatatcg gggttggtgg tcgtgaagtc ggctttatgg
    21841 cggggatgat gaaaaagctc tccaacaata ccgcctgcgt attcaccggt aagggtcttt
    21901 catttggcgg cagtcttatt cgcccggaag ctaccggcta cggtctggtt tatttcaccg
    21961 aagcaatgct aaaacgccac ggtatgggtt ttgaaggaat gcgcgtttcc gtttctggct
    22021 ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga atttggtgct cgtgtgatca
    22081 ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg attcacgaaa gagaaactgg
    22141 cacgtcttat cgaaatcaaa tccagccgcg atggtcgagt ggcagattac gccaaagaat
    22201 ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt accggttgat atcgccctgc
    22261 cttgcgccac ccagaacgaa ctggatgttg acgccgcgca tcagcttatc gccaatggcg
    22321 ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat cgaagcgact gaactgttcc
    22381 agcaggcagg cgtactgttt gcaccgggta aagcggctaa tgctggcggt gtagcaacgt
    22441 caggcctgga aatggcacaa aacgctgcgc gcctgggctg gaaagccgag aaagttgacg
    22501 cacgtttgca tcacatcatg ctggatatcc accatgcctg tgttgagcat ggtggtgaag
    22561 gtaagcaaac aaactacgtg cagggcgcga acattgccgg ttttgtgaag gttgccgatg
    22621 cgatgctggc gcagggtgtg atttaagttg taaatgcctg atggcgctac gcttatcagg
    22681 cctacaaatg ggcacaattc attgcagtta cgatctaatg taggccgggc aagcgcagcg
    22741 cccccggcaa aatttcaggc gtttatgagt atttaacgga tgatgctccc cagggaacat
    22801 ttcttatggg ccaacggcat ttcttactgt agtgctccca aaactgctta tcgtaacgat
    22861 aacacgcttc aagttcagca tccgttaact ttctgcgata gcagcagata tgccagtaaa
    22921 gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac tcgtgcgcct
    22981 ttcgagaggc aagcattata taatgccagg ccagttcttc ttcaattgtc ccgttttgaa
    23041 aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt agcttcgccg
    23101 aggatttacc ggagctatga ttagcgcaat cagagatata gtctgaggga aaaacagcaa
    23161 atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct ttaaaggtat
    23221 tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataactt tcgctatctt
    23281 cgatacccca ttgatcctct aaagactcgc gtcttttact tatgatatcg atcgagtcaa
    23341 aaggaagcac atgatattgg aaggtatctt tgccaggttc aggctttcgc ggccagaact
    23401 ccagcgtttc agaccattgc ttatgataga atcgataagg tgcgatcaat tgtagcgcct
    23461 gtaacttctc gatactgagc ggctcaatac ctttagcctg ataataatgc agttgttctt
    23521 tttttgcttt aaaaccggcc cgaacaataa gccccatcgt aattaataga taaagaaaag
    23581 agcatcccgc ggtaatcaag cctctttcat tcaaaccgtt ggatgttatc gctgcgaata
    23641 caaacattac agcgacaaca catgttaaat aaaaccccca cttacaaagc agcatggcct
    23701 tattttcttt aatcatccgt tcaaaattac tattaaatat ttcccagcca ttaaaagaat
    23761 acttctcgct cccagggtgg ttttgtaata aaactttttt catcacattc cctgttatta
    23821 catactttag aggcgataat tttatccatg caaaaaaata tccaacgaat tttcttgatc
    23881 cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg
    23941 ccccgcttcc ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag
    24001 cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca
    24061 ccggagtacg tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa
    24121 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
    24181 tcatgtccgg tcgcgtagcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg
    24241 tggagtggat atagcgccct tttttagtga ggaaaccacg cttgaacaac agttcaataa
    24301 tcccggcacg cgttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat
    24361 ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa
    24421 aatggcgagg cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat
    24481 cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
    24541 ctaacagcgt gcgccagcct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt
    24601 tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggctg aactgcatca
    24661 agtactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
    24721 aactccgtgc ggtcgggatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc
    24781 ggttgcgtat atctggatct accactggct gcggcaacag atctggtgca tgaacgctga
    24841 tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg
    24901 aacgcggata agtgattagc ttgtgtgttt cgtacagctt ctggcagata tcaagcacgt
    24961 tctgcgcact cagaccaaaa cgttttgccg cttcaatctg caacgccgaa agcgaaaaag
    25021 gcagcggcgc ggattctgat tcccgtttat cgttatagct agtgactata gccggttgac
    25081 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
    25141 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
    25201 gtgtcacgat atgcgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
    25261 catcgcggcg caccaccagc ccaagcacgg gcgtctgcac gcgtcccacg gaaagtacgc
    25321 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca
    25381 gccagtcggc acgcgctcgc gccagcgcag aaacgcacag cggtacaaac tcactgttgg
    25441 aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac
    25501 gctgtacctg ctggcgcttt tccggtgcca gttgtagata gtccagcact tcatccacca
    25561 gcagttgccc ttcgcgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcaggt
    25621 accgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt
    25681 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
    25741 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
    25801 cgataaagcc atcgcctttc cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca
    25861 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
    25921 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
    25981 gtagctttaa cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt
    26041 tgcggatcgc acagcaaatc gcgcacttca cgcggcattt cacccatcag atgaccgtag
    26101 ctggcaaagt tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc
    26161 cccggcaatt ttgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac
    26221 atttcgctca aatggcccag cagaccaaaa ccagtaacgt cggtcatcgc ttttacgcct
    26281 tcgatgttgg caaaggacgc acctgcgatg ttcatccggc acatcacttc cgtcgccagt
    26341 ccctgatgtt ctggtttcaa cagtgatttt ttctcagccg tggtaagaac gccgatcccc
    26401 agcggtttcg tcaggaacag tttacatccg gcttgtgcgg tactgttttt cttcacccgc
    26461 tcggtcggta cgatccccgt caccgccaga ccaaaaatcg gctccggcgc atcgatggag
    26521 tgaccgccag ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact
    26581 tcgcgggcaa tttctgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata
    26641 atcggtttgc cccccatagc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca
    26701 aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg
    26761 ccattgccca gatcgtacac cgccgcatcg tcgcgggttt cattacccac aagcaaattc
    26821 ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt
    26881 ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg
    26941 gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga
    27001 gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga
    27061 aataagtgac aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcagctgcg
    27121 gcgtaccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac
    27181 gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaac ccctgggcaa
    27241 cggctgccat ttgcatcgcc atgaccgcgc atccggcaga caattcctgt tcccatcgcg
    27301 ggactttatg attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg
    27361 gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt
    27421 ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt
    27481 gcatggactt atggtccggc gcgcgcatac ccgcacgcag gatgttttgc agttgttcac
    27541 ccgttggtgc gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg
    27601 catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt
    27661 ttgttacagc acagtccgca attcctgctg acaagtgtcg gttgggtcat tacgataacc
    27721 acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt
    27781 ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa
    27841 tggtacttaa cctgttcttt atttttctcg tgctggttgg tgtggggatc tggatgcagg
    27901 tcagtggtgg tgattcgaaa gaaacggcca gtcgtggtgc actgctgctg gacatttctg
    27961 gtgtaatcgt cgataaaccc gacagttctc agcggtttag taaattaagc cgccaactac
    28021 ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc
    28081 gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag
    28141 gcggcgacca gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg
    28201 ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt
    28261 tcgccaataa aatttggctg tcaccgcaag gcgtggtgga tttgcacggt tttgctacca
    28321 acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg
    28381 tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgacatgtca ccggcagccc
    28441 gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg
    28501 ctaaccggca gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt
    28561 taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac
    28621 tggcatcgag tgcggaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg
    28681 ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg
    28741 gtgacagcat cggtgtcgtc ttcgctaatg gcccaattat ggatggcgag gaaactcagg
    28801 ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag
    28861 tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga
    28921 ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg
    28981 gcatggcggc atctggtggt tactggattt ctacgccagc taattacatt gtggctaacc
    29041 ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc
    29101 tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggca gatgtttcta
    29161 tcaccagggc actgccgccg gaagcgcagc agatgatgca gttaagcatt gagaatggct
    29221 ataaacgctt tattacgctg gttgctgatg cgcgtcattc gacgccggag cagattgata
    29281 aaatcgccca gggccacgtc tggaccggtc aggacgcaaa agctaacggg ctggtcgata
    29341 gtctcgggga tttcgatgat gcggtcgcca aagcagcaga gctggcaaaa gtgaaacagt
    29401 ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt
    29461 ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg
    29521 cctcggtagc ctctaccgtt aaaagtgaaa gtgacaagct ggccgcgttt aacgacccac
    29581 aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaaatcctg caaaaatgtg
    29641 gtcgatatgc tgtcggccac actcctgaat cggactgtct ttcactccct ctctccacta
    29701 cattcccttg ctcatttagc atctgatatg gagtcatcag tgaacaaggc cccctcttta
    29761 attgccgcta tcgtcctcgg actggggatt agcgcctgtg gttattttgt tggcgatggt
    29821 gttaaacatt taaaaaccaa caaccgttat gtgaatgtgc gcgggctttc tgaaaaagaa
    29881 gttcgcgcag atacggcaga attaacaatt gcgattaact ttaagggtaa tgttcccggc
    29941 gaactgttcc cgaaactgga agacgcgcag aaaaaaattg ttgccgaact caatgctcag
    30001 gggattaacg agaaagagat aatcctgggc caatggacaa gcaagcgaac agattccttc
    30061 tatctgaaag atgatcccac aatgccacgc tataacgcag acgggtcagt gacgataaaa
    30121 acccataatg ttgctgctgt cgaaaaagtc gtcgcgaaat taaacgaact gcaagtcgct
    30181 acggatggcg ctatcgctga gagcaaagtc gcctatcgct ttaatggtat tggcgcactg
    30241 cgcgctgaga tgattgctgc cgccaccaaa gatgcacgca acgccgcact acaatttgca
    30301 acggactcag gtagccaggt ggggtcaatt agcgatgcct cacaaggcgt attccagatc
    30361 tttgccagtg gcagtgatga agatgatcct acggcgatta ataaaacggt acgcgtagtc
    30421 acgaccgtaa cctatgcgtt gcaggattaa ccacttttct ccggcgggac actaatccct
    30481 ccgtcctgcc ggacttttct catatccaca gagtatccag gtgcgctcgc tgcccctcac
    30541 gtatatactt ttgctctttc gatatcattc atatcaatat catgcaaaag aaatcaattt
    30601 acgttgccta cacgggcggg accatcggga tgcagcgttc cgagcagggt tatataccgg
    30661 tgtcaggtca tctacaacgc caactggcgc tgatgccgga attccatcgc ccggagatgc
    30721 cagatttcac cattcatgaa tatacgccgc tgatggattc ttcagatatg acgccggaag
    30781 actggcagca tattgctgaa gatattaaag cgcactatga cgactatgat ggttttgtca
    30841 ttctgcacgg caccgacacg atggcgtata ccgcctctgc gctgtcgttc atgctcgaaa
    30901 atctcggtaa accggtcatt gtgacagggt cacaaatccc gctggctgag ttacgctctg
    30961 acggacaaat taatctgctg aatgcgttgt acgttgcggc gaattatccg atcaacgaag
    31021 taacactctt ttttaataac cgactgtatc gcggcaaccg cactaccaaa gcccatgccg
    31081 atggttttga tgcgtttgcc tctccaaacc ttcctccgtt actggaagca ggtatccata
    31141 ttcgtcgttt gaatacgcca cccgccccgc acggtgaagg ggaattaatc gttcatccaa
    31201 tcaccccaca accaattggc gtagtgacga tttatccagg gatttctgct gacgtcgtgc
    31261 gcaattttct gcgccaaccg gtgaaagcat tgattctgcg ctcctatggc gtgggtaatg
    31321 cgccacaaaa caaagccttc ctgcaggaat tacaagaagc cagcgatcgc ggtattgtag
    31381 tggtcaacct gacacaatgt atgtccggta aagtgaatat gggtggttat gccaccggta
    31441 acgccctcgc ccatgctggc gttattggcg gtgcagatat gactgtagaa gccacgctaa
    31501 ccaaactgca ttacctgctt agccaggaac tggatactga aaccattcgc aaggccatga
    31561 gccaaaacct gcgcggcgaa ctgacgccgg atgattaagg agactgcaat gccccctcgc
    31621 gccctgttac tggtcgattt acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg
    31681 gaaggtgaca gtacggtgga tgtcgctaac cgcctgattg actggtgcca gtcgcgcggt
    31741 gaagcggtta tcgccagtca ggactggcac ccggcgaatc acggcagttt tgccagtcag
    31801 cacggtgtag agccttatac gccaggccaa ctcgacggtt tgccacaaac cttctggcca
    31861 gatcactgtg tgcagaacag tgaaggcgca caattacatc cgttactgaa ccaaaaagcg
    31921 atcgcagcgg tgttccataa aggcgaaaat cctttagttg acagttacag tgcctttttt
    31981 gataacggcc gtcggcagaa aaccacgctc gatgactggt tacgcgatca tgaaatcgat
    32041 gaattgatcg ttatgggcct ggctactgac tattgcgtga agtttaccgt gctggatgcg
    32101 ttacagttag gttataaggt gaacgtgatt accgatggtt gtcgtggcgt gaatattcag
    32161 ccacaggaca gtgcacacgc gtttatggag atgtcagcag ctggggcaac gctatatacg
    32221 ctggcagact gggaagagac gcaggggtaa ttttacgctg gcctacagtt ctgtactggc
    32281 attgtaggcc agataaaaca cgtcagtggt acatctggta atggacgcca tcaacgaaag
    32341 atttagttca cctcagaaat ctcttccagc gacaccttcc gcgtttcgat gccaaaaatg
    32401 gagagaacca gcgcgcagag cactaacatc acaccaagca caataaacac cgtgattgac
    32461 ccataatgtg ttaataatgc cgcaacgcca tagggcgtga agactgcgac gatccggccg
    32521 agggcattaa cgaaacccga accacgcagg cgtaaatgcg ttggccaaag ttccgggata
    32581 taaaccgccg acgcaaagca aacatacatg tataaaaaga agatcatcac cagaccatag
    32641 attaaaatcg cccactctgt agtctgaatt gaatagatat agcctaacac agcaataata
    32701 ataagtaagg cggagccaaa taatcgacga ggaaaatgat caataattaa tgccgcaata
    32761 aatattccta ccggagcgcc aatcataata acagcggtca tgaatattga tttatcgaca
    32821 tcaatgccgg agttaacaaa tatggtcggt atccatacgg taatggtata aagcgaaatg
    32881 ttcattgcaa ttaaaacagt tatcgcaact aatgtacgtc gtaacatttc ccctttaaac
    32941 aggagccaga aagtaccctt gattacgtta accttgctgt tgctctgata cgaagtaagc
    33001 gggggtaaac gaatactctt ctctctttca atttgctgct ctacttcacg aagttggctt
    33061 tctgcaccgg cgatttgccc tttccctgcc agccatcgtg gcgactcgat aaagtattta
    33121 cctgagagaa accaggctaa cagtatgcca ataccaccca acagaaacat tatccgccaa
    33181 ctaaaaaaag cgataaccac cacacctatc gccgcagaca gcatgggcga ccagttacca
    33241 acaaatgaga gccgcgcgga ccattttcca cgcaccgtcg cggggataaa ctccgtaaat
    33301 gaggcatagc caaccataat cagcgccccc attcctgttc ccatcaggaa gcgaaagaag
    33361 atgagccagt acatatcagg gacaaaagcg gcccctgttg cagcaatacc gacgatgaga
    33421 agatttatgc gaaacgccct gcgccgccca aagtaatcac cgatgaaccc accggtaaga
    33481 gagccgatga aataaccaaa cattaatgcc gaggtaaagg cggcattgag gaaattattt
    33541 gaccatccat tgcttaccag ctttgctaag acgacattac ctgagtaact caaaaacccc
    33601 gttaataaca gactaaagct tattatacca aatatacgat aatgaaaccg ggccaaaggc
    33661 aagcggtcca gtcttgcgcc aatttgatca tattgttcca ttgggtgcct cggatttgct
    33721 aaaaaaggta gcaattgcgg caaattcact tgccgcatct ttatcatcat tttatttcca
    33781 ggcacaggaa caaataatat tgacaacgtg tgctaaaatt accacaccag ctggatatta
    33841 ttgtctttgc aaaatgctat ccactctgca cccggtgatt tatcagttat tatgtaatta
    33901 atatgactaa agtcagctaa ctggacaaaa gctttgcgat caaacttaga gtgatcaacc
    33961 aataacgcaa cttctgtcgc ctgacggatc attgtctttt tgatttcagc ttccgcttca
    34021 ttagagtcca gcgcgccgct gttaatatca agacctttac aactcattac catgatatcg
    34081 acatgatagc ggctgatgat ctctttagta attcttcctt gcagtgaaag tgtatttttg
    34141 tttagttccc cacccgttga aacgacttta atttctgact gagccagaac atgaatcgct
    34201 tccgccgagt tggttagcaa cgtcaggtca ctacggtcct gtaacaattt gagcaattcc
    34261 attacggtac tactggaatc ggctgccatg gtggttttat tgtcgataaa gggtagtgcc
    34321 ttgcgtgcaa taagctgctt ctcttcataa aacgatgaag cgcgcttata aaaatggata
    34381 ttctccgtca acatcgctgt atttaaaaca gcaccaccat aggttctggt caaaaagcct
    34441 tcatcttcca gcttctcaag atcgcggcga atggtttctt cggttacctg aaaaatccca
    34501 ctcaaatttg agactgtcac ctttttatcg ttggcaacca tttgcttaat tgcctgaatc
    34561 ctgtcttttg ccgccacgat tacacccctg tatcttttta catcacatta gcgcgattat
    34621 cgcataaccg atgtttactt tcaaaataac ctgtttgaat cacagatttt catcacagtt
    34681 ttcacagaaa cagaggtgaa tcgtgttgag tatttaacgc tccagggcct ctgccatttc
    34741 cctcatcaat gttgcgtctg catccgataa gttgatattc agtgccgcga cattttcgcg
    34801 tacctgttcc ggtgcagtag ccccactaag aatggagatt aaatcactct gttttaatat
    34861 ccacgccagt gccagagtgg gaattgtgca ctgataacga gcacaaagtg gctgccactg
    34921 ttcaagcata tcaatcactt tcagcatgtt ttcacgctgg aaccagactt tatttgcccg
    34981 agcgccgccc ggaacgtaat cacgagtgat ggtgccggtc aacaatccct gctctagcgg
    35041 ggaataaacc tgaaccacaa tgccattatc acgacatagc ggcagcagtt cgttttccat
    35101 tgcccggtcg aggatactgt atttcgcctg aataatatcc agttcaccat attgcagata
    35161 ctcgcggata tggtcagcat cgacgttagc agcgcctata gcgcgaattt tcccttcagc
    35221 ctttaactca ttaagcactg cgacagtttc agcgatcggc gtaaaaaatg gcggcaccga
    35281 ctgccagtgc gtcatgtaga tatcgatgta atcaataccc agacgttgca agctggcttc
    35341 tacctcttcg cggatagatt ccggggaaag gtttttatac aactgccgat cgccaacttt
    35401 gttgaataaa cttccttttc gttcccagac aatgccgcat ttggtttcta ctacaacctg
    35461 ttcacggggc aattttttta acgcctgacc gacgataact tcactattgc caaagttata
    35521 tcctggtgca gtatcaatca ggttaatgcc gcaacgatgg gcttcaagaa tcgtatcaat
    35581 acatatttgc cgatcgagag cgccattcca tgcaggaccg ccgccaatgg cccatgtccc
    35641 caaccccatt cgcgaaagcg taatatccgt tgtgcctaaa ggtatctttt tcatttgcct
    35701 tcattatcct tcgtattctt ccagcaattg ttcgaccagc tttctgtttt ttacgccggt
    35761 ggtggcaccg acgcttagaa ccgagatagc cgccgttgca ttggcaaagc gtgcgcattc
    35821 acgcagattt tttccttcta acagtgccgc aataaaacct gaagcaaagt tatcgcccgc
    35881 gccaatggtg tcgatggcgg ttattcctgc gaccgccggc accttcatcg tcatgtcacc
    35941 acgcttgata aagcagccgt ctttacccgt tttaatcacc accgttttta cgccgcacgc
    36001 aagaaagcag tcagcaattt catccagtgt ctctttcccg gtgagtaatt ttgcctcggc
    36061 ataattagga aacagataat cgacataact caatgcttcg caaatatcat ccagcgtttc
    36121 attcaaccgc ggtttgatca tatcggcaca gataatcatc tgccgggctt tagcttgcgt
    36181 aaaaatttct gttagcgctt taccatccaa tagtggactg ttgaaaatac tggccagtga
    36241 taataatttc gcctgggaaa accgcgcaaa atcaacatcg tcaatattca atttccacag
    36301 actgccgtta cggtttgtga caaacgtccg ctcgccatcc tccgtcacca gtccaacatt
    36361 aatagaggta tctatgttga cgtcctgctt caggctttga atatcaatat tctctttgcg
    36421 gcaatggtcg agaataaact gccctgcggc atctttacca atacggctca ttaatgctgt
    36481 acgatggccc agacgagaaa taattgttgc ttcatttatt gcatcacccc cggtggtcat
    36541 tgcgattctt tcaagaggat aagaatccac atcaaagata tttttactga ccggttgcaa
    36601 tggaatatca acaatagcgg cacctataca aataacgtcg agattatcca tatcattatt
    36661 ccgctttgcc atcagaaccg aacagtttaa ttttttccaa tgcccgttct tttacagctt
    36721 tacgtacttc acgttctaaa tgcaggaaag gctgatcctg gttttctttc actgcgacca
    36781 ttgcggcctg acagagctcc gtatggatgt tgattttcgc gatacccaac gaaattgcag
    36841 ttttaatgtc ggcatcactg atccccgatg cgccatgcaa aaccagcgga acagacacgg
    36901 catcgcgtac gcgtttgacg acctcaaagt tcaattgtgg ctctgacgta taaaccccat
    36961 gctggttgcc gatggcgaca gccaatgagt cacagcccgt acgttcaaca aattcagccg
    37021 cctggtcagg atcggtatag tgatagcccg ccagcgcttc ctcataaacg gtttcattac
    37081 cgacatgccc taattccgcc tctaccggaa tacccagcgg atggaagaaa tcgacagcct
    37141 ctttggttaa gcgaatattt tcttcgaaat caaatgcgga agcatcacgc attaatgaat
    37201 tcataccatg agtccaggcg ttatgaataa tctccatact ccgaccatga tcccaatgag
    37261 ttattaccgg caccgttgct tttcgtgcca ttgataccat catgtgagag aaatcttcaa
    37321 atgaggtgtt gccgacaaaa cctgtaccaa aagaaataat aaccggtgat ttcgcttctt
    37381 cggctgcgtc gataacgccc atcaacattt ctgcattcca tacattaaaa tgggcaatag
    37441 cataacgttt atttgtggca tcgttttccc aatatctgat atctgcgagc atgttctgtt
    37501 ctcccgtatt aattaatcgc taatcttaat aacgccttta ataatgtcgc gtttattgtt
    37561 aactgactct tcaaatgcct gttgtacatc ccgataatcg taaatatgcg tcaccatcga
    37621 tttcacatcg aatcgccctg aagaaatagc ttcaatcgtg accggataac gattggcgta
    37681 gcggaatacc gtctggatag taacttcgcg atttattttg aggaaattga ttgccgaatc
    37741 gccgggtaca gtaccaacaa tcataatttt accgccgcgc attaccagat aaggtgcctg
    37801 tttaacggtg accgcagaac ccgctgtttc gaaaacaata tctgcgccca tgtcttcggt
    37861 aaattgctga cagcgtgcaa tagtgtcttc ttttgcgccg ttaataacca ctgtcgcacc
    37921 aagctgttcc gccattgcca gacgtttttc cagcacatca acgacggcaa tttcagttgc
    37981 tcccaggcat ttgcacgctt gcaacgtcat caaaccaata caacctgctc ccagaataat
    38041 tatcttctta cccggtttaa catctgccag catcgcggca tgcatcccga ctgcggcagg
    38101 ctccaccagc gccccttcca tcgtgtccat attgtcgggc agtttgtaag taaagctctc
    38161 cggatgacac agatagtgtg ttaatgcgcc gcggtagttg ggttgtgtcg ccataaaatc
    38221 aacgtccggg cagatgttat atttgccttc cagacagtaa cgacagtgac cgcaaggaac
    38281 gccaggttcg atatttaccc gatcccccgg tttaaatttg cgcacgcggc ttcccacagc
    38341 cacaaccgtc ccggcgcatt catgacccag gccaatttct tgatttgggt ctttaggcgg
    38401 aataaacgga cctgattcaa aaccatgtac atctgaacca caaataccga catattctac
    38461 tttaatcaaa acttcatctt ctttaggcac tggtatttct gctgaaataa ttttcattgt
    38521 gcccggcacc tgcaatattg cttttgaatt tttcattttg aatccacccg tattatttat
    38581 tggctactgc atcaatactt aaactttcaa cggagacacc tttggtttcg attccaatgg
    38641 tggcgatagc aattgcgaca ataattgaaa ccgcccccag aagaataaag actcccgtaa
    38701 cgccataact actgagcagc actgcaacgg cataaggtgc ggcaatacca ctgattcgcc
    38761 ctaccgcatt cgccagaccg gagccacgga gtttggcctc tgtcggccag atctcaggga
    38821 catacactgc cgaggcatag caaacgtaca tatagacaaa agtaatcagg aagaaaccaa
    38881 tcaacgttat gagcaacata ctggtttgca gtgaatagat atatccgagc accgcaatca
    38941 ggattaatag ccccacaccc attgttttac gcggaatttt atccatcacc agcatggcaa
    39001 taaaaatgcc aaatggcgcg ccaaacatac tcatggtatt taaaacaatc gagtctttta
    39061 aattaatccc ctgggtcatg aatatcgttg gcaaccagtt aattagtgtg tactgcacaa
    39121 cgttcatggc aatcagcaca caagagccta atatcacgcg tttcagtaat actcctgtca
    39181 gtaaggctga atacggcacc gcttgtggcg ttttaccgtc atcagcaata accacgggcg
    39241 gcaaaggttt gcccgtctgg tgtatgacgc cttcttctat actccgcatg actttctcgg
    39301 cttcctgata ccgtccgcgc gattccagcc agcgcgggga ttcaggaaag tagcgccagg
    39361 ccagcgccgt agcgataagc gacaatattg cagggataag cagttgtacc cgccagttcc
    39421 actctgcact aatcagcggc gtgagtccca tcgctatcaa tgaacagagc ggatatgacc
    39481 agttgccaat aaaggaaacc cgacttgacc acgttccacg atttctaccg ggcatatatt
    39541 cggtgaaacc agcaaacagc gtaaccagta aagctcccag cccaactccc atcacaaaac
    39601 ggcaggcaat aaggaaatcc atattcggtg agaaagcacc gacaaccatc gaggcaatat
    39661 gaatggcctc atagaggatg aacgcatttc tgcgaccggt tttgtcgcca atgatgccgc
    39721 ccaccagcgc accgagaaac attccggcgg tcgtgattgc tgagaatgtg gctgtggtgg
    39781 aattatctgt ccagcccaac gctttcagct gcgcgaggat caagccacca acggcattac
    39841 tccagcagac aagcaagcca aacgcgacga tggcaaacat tgacgaatgc cagcggcaat
    39901 ccggtaagcg atccagccgt gcaccacaat gcggttttgt tatctgttcc atgcgataac
    39961 gttccttcag gaaagttgtt attcatcaaa gtcgtaagtc atgatcactt tgattgcggt
    40021 tttatcgacc atcgcatcaa acccttcgcg ccattgcgac aggccgatac gatgcgtgat
    40081 catcggtttg actttgatag cgccgctggc taatagcctg atagcgttac gccatgaggt
    40141 ggagtcatag gccatatgcc caatgatgct tttgttccag gcggtaatgt cattaatcga
    40201 gaaatcaaga ggtttgaagc ccattccaac gcgtaccact tccccgttcg ggcggagcat
    40261 ttcgatggct tgtttcagtg cgatattggc accggagcat tcaatcacca gtcccagatt
    40321 gtcttttcca caaatttgct ggcagcgcgc caccacatct tcggtagaac catttactac
    40381 tgccgtcgca cccagttctt ttgcaaccgg gaagcggacc gccacatctt cttgcagacc
    40441 aacgacgacg atatttaccg cccccataat tcgcgccatt tgtacggaga acagcccgag
    40501 tgggccagtg ccgatgacga ccacatcctg accaggaagg aatttcgatt gctgcgcgat
    40561 ggatttgtag gcattacaga tagggtcaag tacggctgcg tcctcataat caacaccatc
    40621 agggatttcc cacaacgcat gacgatgaat tttgagaatt tcaccaggaa ccagacaata
    40681 tttggaaaaa ccaccgcccc aggtattatt atccagacca aggtttactt tttctgtaca
    40741 acacagaaaa tcaccttgtt cacaggccgg acaaacgccg caaacgtgac cgctgttatc
    40801 cgatacgacg cgttgcccca ctttccagtc tttgactttt tcaccaacct gcgcaataca
    40861 acctgcgaac tcatggccgc ggatagagtt aaactcatca gaaccgctat cgacattgta
    40921 gtgcttcatg tctgcgccgc agattgccgc ggctttaatt tcaatcacta catcttccgg
    40981 gccacacatg ggttgtggga catcaatcat cttgtagccg ccaaaggctt tgccaaaccg
    41041 agccagtgct ttcattgcgt catcctctgt gttttgtttt gcttagatgg tcatcatact
    41101 tacccacatg atttttgagg tcaacattaa atctgtaaaa cacagataat gatctgcgtt
    41161 ttacaactca gatcacaatt acgcagaaaa tggttggttc tgttacaggt tgatggaagg
    41221 ctgaacgcaa aaggagcaaa tttgagaggg attaccggcc aaataggtct gagacttaag
    41281 ttcaggagaa gtcattcagg cgtgaacgcg acggcaagag accgggctgg ggctttgcct
    41341 ggtcgtgacg agataattga gatgagtcgt ggggttattg ttggctatcc cctttataac
    41401 cgcggcaaaa aggggacaaa ccacttactt aaacatggca ataggctttg ctgagatacc
    41461 aaaatcttct tttaactgtt gtttgctctt catgaccatc tgaccatttg tgtcaattgt
    41521 catatgctgt gcttcggtat tattgcgggc ttgccacagc atcaccagtt gcaggcagtt
    41581 ctctttttgt tcctcggtca acgcaacgcc atcaggccat ttccccagtt caacggcggt
    41641 cgacaaacgc tggtatacct caggcgtcat gctgttgata atgtcatcaa gattcataat
    41701 ttttccgctc ctgtggaata agttgctgaa tcgttttttc aaccattgat ttcttcgccg
    41761 ttttcgccat cagtgaagcg taaagaagca gagttaacac aataacgttc gcccgttggc
    41821 tgcggcccgt cggggaagac atgccccaga tgggcatcac agttaccgca acgaatttct
    41881 atgcgctgca ttccatgtga caagtctttg atataacgaa tggattcttc actcaccggt
    41941 tcgtagaaac tgggccagcc acagccggaa tcatacttgg tttgggaatg aaacagcggg
    42001 gcatcgcaga tcaaacagtg atatacgccg tcacgcttgt tatgcagtaa acgacccgta
    42061 aatggcggct ctgtcccatg attctgcgtc acgtaaaact gcatctcgga caaatttttt
    42121 ttcagttctt ctgccgaagg tttattagcc atttgctcac atctcacttt aatcgtgctc
    42181 acattacgtg actgattcta acaaaacatt aacaccaact ggcaaaattt tgtcctaaac
    42241 ttgatctcga cgaaatggct gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt
    42301 gccctttaaa attcggggcg ccgaccccat gtggtctcaa gcccaaagga agagtgaggc
    42361 gagtcagtcg cgtaatgctt aggcacagga ttgatttgtc gcaatgattg acacgattcc
    42421 gcttgacgct gcgtaaggtt tttgtaattt tacaggcaac cttttattca ctaacaaata
    42481 gctggtggaa tatatgacta tcaaagtagg tatcaacggt tttggccgta tcggtcgcat
    42541 tgttttccgt gctgctcaga aacgttctga catcgagatc gttgcaatca acgacctgtt
    42601 agacgctgat tacatggcat acatgctgaa atatgactcc actcacggcc gtttcgacgg
    42661 taccgttgaa gtgaaagacg gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc
    42721 tgaacgtgat ccggctaacc tgaaatggga cgaagttggt gttgacgttg tcgctgaagc
    42781 aactggtctg ttcctgactg acgaaactgc tcgtaaacac atcaccgctg gtgcgaagaa
    42841 agtggttatg actggtccgt ctaaagacaa cactccgatg ttcgttaaag gcgctaactt
    42901 cgacaaatat gctggccagg acatcgtttc caacgcttcc tgcaccacca actgcctggc
    42961 tccgctggct aaagttatca acgataactt cggcatcatc gaaggtctga tgaccaccgt
    43021 tcacgctact accgctactc agaaaaccgt tgatggcccg tctcacaaag actggcgcgg
    43081 cggccgcggc gcttcccaga acatcatccc gtcctctacc ggtgctgcta aagctgtagg
    43141 taaagtactg ccagaactga atggcaaact gactggtatg gcgttccgcg ttccgacccc
    43201 gaacgtatcc gtggttgacc tgaccgttcg tctggaaaaa gctgcaactt acgagcagat
    43261 caaagctgcc gttaaagctg ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga
    43321 agatgacgta gtatctaccg atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa
    43381 agctggtatc gctctgaacg acaacttcgt gaaactggta tcctggtacg acaacgaaac
    43441 cggttactcc aacaaagttc tggacctgat cgctcacatc tctaaataag ttgagatgac
    43501 actgtgatct aaaaagagcg acttcggtcg ctcttttttt tacctgataa aatgaagtta
    43561 aaggactgcg tcatgattaa gaaaattttt gcccttccgg tcatcgaaca aatctcccct
    43621 gtcctctccc gtcgtaaact ggatgaactg gacctcattg tggtcgatca tccccaggta
    43681 aaagcctctt ttgcattaca gggcgcacac cttctctcgt ggaaacctgc gggtgaagaa
    43741 gaagttctgt ggttgagcaa caacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc
    43801 gtaccggttt gctggccgtg gtttggtccg gcggcacaac aaggcctgcc tgcgcacggt
    43861 tttgcccgca acctgccgtg gacgctgaaa tcacatcgtg aagatgctga tggcgtagcg
    43921 ctgacttttg aattgacaca aagcgaagag acgaaaaaat tctggccgca cgactttacg
    43981 ctgttagcgc atttccgcgt gggtaaaacc tgtgaaatcg atcttgaagc acacggcgaa
    44041 tttgaaacca cctctgccct gcatacctac tttaacgtgg gtgatatcgc taaggtaagc
    44101 gtcagtgggc tgggcgatcg cttcattgat aaagtaaatg acgcgaaaga agatgtactg
    44161 accgacggta ttcagacctt ccctgaccgt accgatcgcg tatatctgaa tccgcaagat
    44221 tgcagcgtga ttaatgatga agcgctgaat cgtattatcg ccgtaggcca ccagcatcat
    44281 ctgaacgttg taggctggaa cccgggaccg gcgctttcag ttagcatggg cgatatgccg
    44341 gacgatggct acaagacctt tgtttgtgta gaaacggctt acgcttcaga aacgcaaaaa
    44401 gtgaccaaag aaaaacctgc acatctggcg caatccattc gcgttgcgaa acgttaattt
    44461 acgttaatgt tgtgtgccgg gtgcgatgca tccggcacgt atcaacatca caccatatcc
    44521 agcgcagttt ttccttttgg tgccggatat gctttgtcca gcatggctaa ttccgctgac
    44581 gaaagttcga cctcaagcac agccgcattc tgttggacat gggcaatcgt ggccgctttt
    44641 ggaatcgcca tcacaccctg atggctgatt acccacgcca acaatacttg tgccgcgctg
    44701 atattgtgag catgtgcaat ttcgttgact accgcgtttt ttaacagtcc attgcgcaac
    44761 cgcccggcct gggctaacgg actgtaagcc atcaccggca tctgctgttg ctggcaccag
    44821 gggagtagat cgtactcaat ccctcgtgaa ccgagatggt aaagcacctg attagtggca
    44881 cactgatttc cccccgtcag ctgccagagt tcctgcatat cagcataatc aaggttcgaa
    44941 acgccccagc ggcggatttt tccttgggcg atcaattttt ccatcgctgc gacagtctct
    45001 tcaaaagcga aactgccaga ccagtgtaat aagtagagat cgagataatc agtattgaga
    45061 cggcgtaaac tggcttcgca tgcatttatc gctttttgcc cgccagcatt ccacggatag
    45121 actttagaga cgagaaagac gttatcacgc agaccggtta atgcttcccc aaccaccttt
    45181 tcggcaccac catcggcata catttcggcg gtatcaatga gggttaaacc gagctcaatg
    45241 cccgcgcgta gtgcagcaac ttctgtttta cacttactgg catcttcgcc catataccat
    45301 gttccctgcc ctatggctgg cagtgagaca tcgccactaa attgaatcat tttttgttgc
    45361 attgtttcct cccaggtatt gcaccaccgt aatgcaaaac agggcgtgac gccctgtttt
    45421 tatgcacaaa atgccctgga aagatgcatt atcagaattt gtaggtgatc ccggtagaaa
    45481 tcaggccagt ccaggattta tccaccatcg ggctgtcagt aacttcatca gacagacggg
    45541 tgtagcgcgc ggtaccgtaa acactccagt cgccgaggaa gttgtagctg gcgctcagct
    45601 ccaggtaagg gctccagctg tcgttcgggt tatagccacg cagaccgctg cgagcggact
    45661 ctttgcgcga tacgccataa tagtattcgt tctggttttc gctgttccac tgcacaccaa
    45721 tacccggagt cacggtcagg ccaccgttgg tgtaacgata caaccaggcc atatcccaga
    45781 cgatgccgtt gctgttatcc agggtatcgc cagccagggt ggtacgcagg taaccgtact
    45841 gggtaaagtg agcataagac agaccagcca tcatggtgct cttacggtca tccaggtgac
    45901 gcatttggtg atcgccactg tctttagctt tgaagtaaag cggcgaccag taagcggtaa
    45961 ttgaaagttt atccgttgcg tcattccaca ggtagtaacc accacctaag ccacggaacc
    46021 agaagttatc gccttcatag ttgattaccg gtactgggta aacatcggta tcgtaatctt
    46081 tatatgggtg ttcaacgaca cctacgcctg cgcccaggga aaatttacct tcagcgtgcg
    46141 ctacgcctgc agacgttgcg ataagcactc caagtgccag aagtttgagt ttggtcacaa
    46201 ttaatcattc cttaaacaaa tgtttagcgg gcgacaaagt ttacccgtca atacatcgaa
    46261 acccaacctt tttacgtttt cattttttaa agtaactgtt taattttcct gacgcggatg
    46321 acaccgcgct tacagccaaa tgaatttaac gttactggca accctggtct ttacattaat
    46381 tatgcaaaat ttatggatga gttgttgata tgccattgaa attaagaaag ccgtgcaggc
    46441 aagttttcca tttgccatct acgcttaatt ttgaaggtgt atcaccgggc acgttgttct
    46501 catcgtcgat aaaatggcat gagagttgct gtgttttagc aagagacgtc gttcagttta
    46561 cctcttccgg gagcctctac tattcatatg aacggctctt aacctgtgct aaaaaacgaa
    46621 aggacggcat accatgaata tattcgatca ctatcgccag cgatatgaag ctgccaagga
    46681 cgaagagttc acactgcagg agtttcttac cacttgtcgg caagatcgca gtgcttatgc
    46741 caacgcggct gagcggctat tgatggctat cggtgagcct gtcatggtcg atacagccca
    46801 ggaacccaga ctttctcgac tcttttctaa ccgggtcatt gcacgttatc cggcatttga
    46861 agagttttac ggcatggaag acgcgattga acagattgtc tcttatctga aacacgcggc
    46921 tcaggggctg gaagagaaga aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa
    46981 atcatcgctt gctgagcgac tgaaatcatt aatgcagctc gtaccgattt atgtattgag
    47041 cgccaacggt gagcgtagcc cggtcaacga tcatccgttc tgtcttttca atccgcagga
    47101 agatgcgcag attctggaaa aagagtatgg cattcctcgc cgctatctcg gcaccatcat
    47161 gtcgccgtgg gcggcaaaac gtctgcatga atttggtggc gatattacta agttccgggt
    47221 agtgaaggtc tggccgtcca ttctgcaaca aattgctatc gccaaaacag agcccggtga
    47281 tgagaacaac caagacatct ccgcgctggt agggaaagtc gatattcgta aactcgaaca
    47341 ctacgcgcag aatgacccag acgcctatgg ctattccggc gcactgtgcc gcgccaacca
    47401 ggggatcatg gaattcgttg agatgtttaa agcaccgatt aaagtgctgc atccgctgtt
    47461 aaccgccact caggaaggta actacaacgg gacggaaggt atctccgccc tgccgttcaa
    47521 cgggattatt cttgcccact cgaacgagtc cgaatgggtc actttccgta ataacaaaaa
    47581 caacgaagcc ttcctcgatc gtgtttacat cgtgaaggtg ccgtattgct tgcgcatttc
    47641 cgaagagata aaaatctacg agaaattgct taatcacagt gaattgactc acgccccatg
    47701 cgcccctggc acgctcgaaa cactgtcacg tttttccatt ctttcgcgcc tgaaagagcc
    47761 agaaaactcc agcatttatt caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac
    47821 cgatcccaaa gccaagtcgt atcaggaata tcgtgactac gccggtgtcg atgaagggat
    47881 gaacggtctg tcgacgcgtt ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca
    47941 tgtagaagtg gcggcaaacc cggtccatct gttctacgtc ctggaacagc agatcgagcg
    48001 cgagcagttc ccacaagagc aggcagaacg ctatctggag ttcctgaaag gttatctgat
    48061 cccgaaatat gccgagttta ttggcaaaga gatccagacg gcctaccttg aatcctattc
    48121 cgaatatggg caaaacattt tcgaccgtta tgttacctac gcggatttct ggattcagga
    48181 tcaggagtat cgcgatccgg ataccgggca gctgtttgac cgcgagtctc ttaacgccga
    48241 gctggagaaa atcgagaaac cggcggggat cagtaatcca aaagatttcc gcaacgagat
    48301 tgttaacttc gtactgcgcg ccagagccaa taacagcgga cgcaatccga actggaccag
    48361 ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc tccaataccg aggagctgtt
    48421 gccggttatc tcgtttaacg ccaaaacgtc aaccgacgag cagaagaaac acgacgactt
    48481 tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg
    48541 gtatttgcgc gtacgtaaat cgtcttaaca accctggccc ggtacacatg ctaccgggcc
    48601 tacaacgaca gcgaaccgtg ggccagagaa atggcaacac aggcgtagca tacagttggc
    48661 aaatgtagta cggggggcat atgacctggt ttattgaccg gcgtctgaac ggcaaaaaca
    48721 aaagcatggt gaatcgccag cgttttttac gccgttataa agcgcaaatt aaacagtcga
    48781 tctccgaggc cattaataag cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca
    48841 ttcccacgga agatattagc gaaccgatgt ttcatcaggg gcgtggcggc ctgcgccacc
    48901 gcgtacatcc gggcaatgac catttcgtcc agaacgaccg aattgaacgt ccccagggtg
    48961 gcggcggagg ttccggcagt ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg
    49021 aattcgtctt tcagatttcg aaagatgagt atcttgatct gctctttgaa gatttggcct
    49081 taccgaatct gaaacaaaac caacaacgcc agctgaccga atataaaacg catcgggcgg
    49141 gttataccgc taacggcgtt ccggccaata tcagcgttgt gcgttccttg cagaactcac
    49201 tggcgcgacg cacagccatg acggcaggca agcggcggga acttcatgca ctggaagaga
    49261 atttggccat catcagcaac agtgaacctg cgcagctgct ggaagaggaa cgtctgcgca
    49321 aagaaattgc agaattacgt gccaaaattg aacgcgtccc ttttattgac accttcgatt
    49381 tacgttacaa gaactacgag aagcggcccg atccctccag ccaggcagtg atgttttgcc
    49441 tgatggacgt ttccggttca atggatcaat ccactaaaga tatggctaag cgtttttata
    49501 ttctgctgta tctgttcctc agcagaacgt ataagaacgt ggaagtcgta tacatccgcc
    49561 atcataccca ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag gaaactggcg
    49621 gcaccattgt ttccagcgcc ctgaaactga tggatgaggt agtgaaagag cgttataacc
    49681 cggcacagtg gaatatttac gctgcacaag catcggacgg cgataactgg gccgatgact
    49741 ctccgctttg ccatgaaatt ctggcgaaaa aattattacc cgttgttcgt tattacagct
    49801 atatcgaaat tacccgtcgc gcgcatcaga cactgtggcg agaatatgag catctgcaat
    49861 ctactttcga caactttgcg atgcagcaca tccgcgacca ggatgatatt tatccggtgt
    49921 tccgtgaact gtttcataaa caaaatgcaa cagctaaaga ctaaaactat cagccgggtc
    49981 attatcgcct ggctgatttt tagcttactg taaattatct catttattac atacattagc
    50041 ttacaatcgc tttaaatatg acagcataac ctttacataa tttagttcca gaaaacaatc
    50101 attcggaaaa atgattcagt caacacgtat ttccatgggg ttattcttta aatatttttt
    50161 atcgttaacg aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa taaaatccag
    50221 cactcacatt gctctccttt ttatggtttc tatgggtaca caaaaattaa aagctcaaag
    50281 cttttttatt ttcagtttat tgctgacgtt gattttattt tgcattacta ccttatataa
    50341 cgaaaacaca aatgtaaaac tcatcccaca gatgaattac c
//
LOCUS       DAZGCQ010000028        46140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000028 DAZGCQ010000000
VERSION     DAZGCQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.028"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..279
                     /locus_tag="ACHK07_001716"
     CDS             97..279
                     /locus_tag="ACHK07_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102060.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
     gene            complement(1224..1502)
                     /locus_tag="ACHK07_001717"
     CDS             complement(1224..1502)
                     /locus_tag="ACHK07_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO8102061.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQASDALKQSMSAAGIK"
     gene            complement(2256..2504)
                     /locus_tag="ACHK07_001718"
     CDS             complement(2256..2504)
                     /locus_tag="ACHK07_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO8102062.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(2730..2825)
                     /locus_tag="ACHK07_001719"
                     /pseudo
     CDS             complement(2730..2825)
                     /locus_tag="ACHK07_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2822..3388)
                     /gene="yecD"
                     /locus_tag="ACHK07_001720"
     CDS             complement(2822..3388)
                     /gene="yecD"
                     /locus_tag="ACHK07_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8102063.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLHRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3698..5470
                     /gene="aspS"
                     /locus_tag="ACHK07_001721"
     CDS             3698..5470
                     /gene="aspS"
                     /locus_tag="ACHK07_001721"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO8102064.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            5588..6040
                     /gene="nudB"
                     /locus_tag="ACHK07_001722"
     CDS             5588..6040
                     /gene="nudB"
                     /locus_tag="ACHK07_001722"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO8102065.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            6069..6809
                     /gene="yebC"
                     /locus_tag="ACHK07_001723"
     CDS             6069..6809
                     /gene="yebC"
                     /locus_tag="ACHK07_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8102066.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6844..7365
                     /gene="ruvC"
                     /locus_tag="ACHK07_001724"
     CDS             6844..7365
                     /gene="ruvC"
                     /locus_tag="ACHK07_001724"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO8102067.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(7367..7969)
                     /gene="yebB"
                     /locus_tag="ACHK07_001725"
     CDS             complement(7367..7969)
                     /gene="yebB"
                     /locus_tag="ACHK07_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO8102068.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            8040..8105
                     /gene="yobI"
                     /locus_tag="ACHK07_001726"
     CDS             8040..8105
                     /gene="yobI"
                     /locus_tag="ACHK07_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO8102069.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8244..8855
                     /gene="ruvA"
                     /locus_tag="ACHK07_001727"
     CDS             8244..8855
                     /gene="ruvA"
                     /locus_tag="ACHK07_001727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO8102070.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            8864..9874
                     /gene="ruvB"
                     /locus_tag="ACHK07_001728"
     CDS             8864..9874
                     /gene="ruvB"
                     /locus_tag="ACHK07_001728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO8102071.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(10088..10873)
                     /gene="znuB"
                     /locus_tag="ACHK07_001729"
     CDS             complement(10088..10873)
                     /gene="znuB"
                     /locus_tag="ACHK07_001729"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO8102072.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(10870..11625)
                     /gene="znuC"
                     /locus_tag="ACHK07_001730"
     CDS             complement(10870..11625)
                     /gene="znuC"
                     /locus_tag="ACHK07_001730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO8102073.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            11704..12636
                     /gene="znuA"
                     /locus_tag="ACHK07_001731"
     CDS             11704..12636
                     /gene="znuA"
                     /locus_tag="ACHK07_001731"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO8102074.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            12652..13974
                     /gene="mepM"
                     /locus_tag="ACHK07_001732"
     CDS             12652..13974
                     /gene="mepM"
                     /locus_tag="ACHK07_001732"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO8102075.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            14094..15065
                     /gene="lpxM"
                     /locus_tag="ACHK07_001733"
                     /gene_synonym="msbB"
     CDS             14094..15065
                     /gene="lpxM"
                     /locus_tag="ACHK07_001733"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO8102076.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLMIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(15196..16638)
                     /gene="pyk"
                     /locus_tag="ACHK07_001734"
     CDS             complement(15196..16638)
                     /gene="pyk"
                     /locus_tag="ACHK07_001734"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO8102077.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16766..17635)
                     /gene="hexR"
                     /locus_tag="ACHK07_001735"
                     /gene_synonym="yebK"
     CDS             complement(16766..17635)
                     /gene="hexR"
                     /locus_tag="ACHK07_001735"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO8102078.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            17973..19448
                     /gene="zwf"
                     /locus_tag="ACHK07_001736"
     CDS             17973..19448
                     /gene="zwf"
                     /locus_tag="ACHK07_001736"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO8102079.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            19683..21494
                     /gene="edd"
                     /locus_tag="ACHK07_001737"
     CDS             19683..21494
                     /gene="edd"
                     /locus_tag="ACHK07_001737"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO8102080.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            21531..22172
                     /gene="kdgA"
                     /locus_tag="ACHK07_001738"
                     /gene_synonym="eda"
     CDS             21531..22172
                     /gene="kdgA"
                     /locus_tag="ACHK07_001738"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO8102081.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(22228..23406)
                     /gene="purT"
                     /locus_tag="ACHK07_001739"
     CDS             complement(22228..23406)
                     /gene="purT"
                     /locus_tag="ACHK07_001739"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO8102082.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            23540..23830
                     /gene="yebG"
                     /locus_tag="ACHK07_001740"
     CDS             23540..23830
                     /gene="yebG"
                     /locus_tag="ACHK07_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO8102083.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            23897..24253
                     /gene="yebF"
                     /locus_tag="ACHK07_001741"
     CDS             23897..24253
                     /gene="yebF"
                     /locus_tag="ACHK07_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO8102084.1"
                     /translation="MKKRGAFLGLLLVSVCASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVVWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            24579..25238
                     /gene="yebE"
                     /locus_tag="ACHK07_001742"
     CDS             24579..25238
                     /gene="yebE"
                     /locus_tag="ACHK07_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8102085.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            25447..27507
                     /gene="ptrB"
                     /locus_tag="ACHK07_001743"
     CDS             25447..27507
                     /gene="ptrB"
                     /locus_tag="ACHK07_001743"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO8102086.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(27504..28166)
                     /gene="exoX"
                     /locus_tag="ACHK07_001744"
     CDS             complement(27504..28166)
                     /gene="exoX"
                     /locus_tag="ACHK07_001744"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO8102087.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(28190..28846)
                     /gene="yobB"
                     /locus_tag="ACHK07_001745"
     CDS             complement(28190..28846)
                     /gene="yobB"
                     /locus_tag="ACHK07_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO8102088.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(28948..29178)
                     /gene="holE"
                     /locus_tag="ACHK07_001746"
     CDS             complement(28948..29178)
                     /gene="holE"
                     /locus_tag="ACHK07_001746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO8102089.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            29317..29691
                     /gene="yobA"
                     /locus_tag="ACHK07_001747"
     CDS             29317..29691
                     /gene="yobA"
                     /locus_tag="ACHK07_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO8102090.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWRVVSVDGHKTKGHYTFSVK"
     gene            29695..30567
                     /gene="copD"
                     /locus_tag="ACHK07_001748"
     CDS             29695..30567
                     /gene="copD"
                     /locus_tag="ACHK07_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO8102091.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            30580..30921
                     /gene="yebY"
                     /locus_tag="ACHK07_001749"
     CDS             30580..30921
                     /gene="yebY"
                     /locus_tag="ACHK07_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO8102092.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            31317..31973
                     /gene="pphA"
                     /locus_tag="ACHK07_001750"
     CDS             31317..31973
                     /gene="pphA"
                     /locus_tag="ACHK07_001750"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8102093.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(31974..32165)
                     /gene="yebW"
                     /locus_tag="ACHK07_001751"
     CDS             complement(31974..32165)
                     /gene="yebW"
                     /locus_tag="ACHK07_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO8102094.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(32270..32506)
                     /gene="yebV"
                     /locus_tag="ACHK07_001752"
     CDS             complement(32270..32506)
                     /gene="yebV"
                     /locus_tag="ACHK07_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO8102095.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(32624..34063)
                     /gene="rsmF"
                     /locus_tag="ACHK07_001753"
     CDS             complement(32624..34063)
                     /gene="rsmF"
                     /locus_tag="ACHK07_001753"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO8102096.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(34143..36776)
                     /gene="yebT"
                     /locus_tag="ACHK07_001754"
     CDS             complement(34143..36776)
                     /gene="yebT"
                     /locus_tag="ACHK07_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO8102097.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(36745..38028)
                     /gene="yebS"
                     /locus_tag="ACHK07_001755"
     CDS             complement(36745..38028)
                     /gene="yebS"
                     /locus_tag="ACHK07_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO8102098.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            38158..38655
                     /gene="msrC"
                     /locus_tag="ACHK07_001756"
     CDS             38158..38655
                     /gene="msrC"
                     /locus_tag="ACHK07_001756"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO8102099.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            38752..39450
                     /gene="proQ"
                     /locus_tag="ACHK07_001757"
     CDS             38752..39450
                     /gene="proQ"
                     /locus_tag="ACHK07_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO8102100.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            39470..41518
                     /gene="prc"
                     /locus_tag="ACHK07_001758"
     CDS             39470..41518
                     /gene="prc"
                     /locus_tag="ACHK07_001758"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO8102101.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            41710..42591
                     /gene="htpX"
                     /locus_tag="ACHK07_001759"
     CDS             41710..42591
                     /gene="htpX"
                     /locus_tag="ACHK07_001759"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO8102102.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(42641..44014)
                     /gene="yebQ"
                     /locus_tag="ACHK07_001760"
     CDS             complement(42641..44014)
                     /gene="yebQ"
                     /locus_tag="ACHK07_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8102103.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMADLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMYALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            44191..44982
                     /gene="kdgR"
                     /locus_tag="ACHK07_001761"
     CDS             44191..44982
                     /gene="kdgR"
                     /locus_tag="ACHK07_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO8102104.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(45126..45365)
                     /gene="yobH"
                     /locus_tag="ACHK07_001762"
     CDS             complement(45126..45365)
                     /gene="yobH"
                     /locus_tag="ACHK07_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO8102105.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            45524..45667
                     /gene="mgrB"
                     /locus_tag="ACHK07_001763"
     CDS             45524..45667
                     /gene="mgrB"
                     /locus_tag="ACHK07_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO8102106.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            45742..46029
                     /gene="yebO"
                     /locus_tag="ACHK07_001764"
     CDS             45742..46029
                     /gene="yebO"
                     /locus_tag="ACHK07_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO8102107.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT        11847 a        11201 c        11867 g        11225 t
ORIGIN      
        1 gttggctggg cacaggcgta ataaaaaagg ggggctgata aacagcccct gaccaaagaa
       61 aagtatcagc ggaaacattt agaaggatct aaataaatga ccatgcttgt atacggcgta
      121 aagactgcca cggaaatgtt aattctgtac gtgatgctga tcttgtttcc ggagcatcct
      181 gttgatgtga ataattttca gattttcgga agtgagagtt tggatatttc catatatgcc
      241 acagttgatg tgttttgtgg cgcctggcag tgtgattaag ggaacaggga taacgaaaaa
      301 agcgggggta tcaccacccc ccggtacaag gaagtattaa ctataatgga aacattaatg
      361 aagacattca gtgctatgtt atatggcaaa taaagactgc cacagggaag tttgcagaaa
      421 cagtgatgcc gatcgtgttt ctgtgctctt ctctgataag agtaattttt cagatttccg
      481 gaagtccgta ttttgcacgg gcccggttgt atgcttcaga ctgcggtgga tatcaaggtc
      541 acggagttgg tcggttgcaa ggaatagatt gagaattgag cgtgtttctg gcacatgcct
      601 gactgtaatc atccctacaa actacattga tgccagaaac gcactggtaa cagggcatcg
      661 tgtatttcag tatgtccagt ttttgttgcg gtgattgcat aaaaaatccc ccttgagggt
      721 tgaaatatca ccggaatttt cggcaagtat ttttatcggg ttgagtgtat ttctggggct
      781 ctgctgaggc aattcactga atgtggcttt tgtgctcgct ggaaatgcac tcatacactt
      841 tttaacgcga tttttttcag gcaatagttt ttaatttgtt agcgttctcg ctgtcaggca
      901 cgttttgagc acgtttccga ctctcagcta cagcaatcca cgactaggga cagcacttac
      961 gttttactta gcgcaccgta aaactccgct cttcagggta agggcttaca tgaagaaaaa
     1021 aaataaactt gtgaatatga aagtaaatac tataaaaaga tggttcctct tttttctcac
     1081 gtgctgaata atataaacaa tagtgcatga tacaaaagac actaataacc acaaagttat
     1141 gatacggagc acgatttatg tcttcctgat tcagtgtgtc aagaaacagt tatcaatata
     1201 caataactgt ttcttttatt atattattta attccggcag cgctcattga ttgcttaagc
     1261 gcgtcacttg cttgtttaca ggcagcatcc tggcttgcct cgggcagaga tgagaattgt
     1321 ttctttgatt gttcatattg ttgtttcatt aaattaatct gtccttctgc tactccggtt
     1381 gttttcattt gcgataagaa cttatctgtt tcatcgaaat acttctgaca tgtttctgtc
     1441 atagccgcac tggcagttgt gcaggatatt acagtgatta ataaagcaaa gagacttctc
     1501 atatatcctc cattgttttg tttgaaaaat taacgctatt aaataactca tagacaggta
     1561 aatttatgtg attatagtca taaactataa aaatattgat gttgtctttg tagaaaggtt
     1621 gtgagcaatt atgaaaattt atttgcttgt tcactggttt tagggagttt gatgtgtcca
     1681 tgaagcgttt ctgacgagaa atcaacataa atcaccagtt ggtgaactag gattgccaga
     1741 aacgctcctg tcagttctgt agtgtgtagt gctcacttag agcctggtga actggataat
     1801 gagtcgcacc tcagtatcct gcaatgggct gcttttcccc tgctcctgat tcttttttgt
     1861 ttcaggtctt accaacataa tggttgttgt aataaattgc gtgtgattct gtatcgggtc
     1921 aaccatgtga cgatacatgt tcagtaagga agactgaagg gttcagagtg cgagtatgat
     1981 taatagatgg aatcagcgtt tcaaagattt tgagggaata taaggtctga cctgatgatt
     2041 accttttgcg cctgtatcct ttgcataaag gaggttgatg tagataaagt catctccact
     2101 tgtgatataa aacagtcagt tatgaaaaat cggcataaac atccttttgt taaccttaaa
     2161 gccagactta cggtttttcc atagttacag ataagcggct agtaaggttt tacaggtgat
     2221 aagcctgtaa cggaaatcac ctgcaaatct attaattagt gaataaacca gtcatccgct
     2281 gtttcccaga cttcctgaag cgtttcttgt acaaactctt gcgcggattc tttatccacg
     2341 gctcttaaaa tagtgagtcc atcattgctg gcagatttaa cgataacatc tacatcgtca
     2401 taacgcttac cgacacgtcg tagcatttcc tgttgcaggg caggaacaga accttttggc
     2461 attttgctga ttttttcttt agcgatacag atctcaacac gcataacacc ctcctgtaac
     2521 tgtggttatg tacaggtgtt atttttatct gtatggataa ccagtgtcaa ttcctgatgt
     2581 tgtttatggg gcatcaatgg ggcatgtatg ggacactttt tatcggcgaa attcgtcgaa
     2641 gttcgtcgac atggtaaacg aatcatctat ccaacccttg aaaaacggcg ctcctggacg
     2701 atcttcgtcg atttttaaaa atgttgcgtc acgcagttaa agtggcgggc atactcttca
     2761 aggcgggtga tccccaaccg cacccattta ggatgcgacc attgcggtag accgatgtaa
     2821 atcataacgc gtgaaggatc tcttccacgc tacgcacacg ggcgatgcgc gggtagatat
     2881 gattaatgct gttattgtgt tgctcggcgc tggcggcact acaggcgtct tcggcaatca
     2941 ccagattaaa gccaagttcc caggcattgc gggcggtgga ttcaacaccg atattggtcg
     3001 agatcccaca taacactatt gtatcgatac ctcggcggtg taattgcaac tccagatccg
     3061 taccgtaaaa cgcaccccac tgacgtttga ttatttcgat atcactgtca gttgcaccta
     3121 atgcagctgg atgttgccac cagttttcgg gcaacacttt agcgggggaa ggggcatcaa
     3181 ccggctgttt taatgcttcg gcgtaatcgg cagaccagcc aacgcgcacc agaaacacgg
     3241 gctgaccgct ggcgcgaaat ttcgccgcca gcttcccggc gcgattaact acctcatcgg
     3301 cagtatgtgg accaccggca aaaggtaaga tgccttcttg taaatcgatc accaccagcg
     3361 cagtggtttt agcattaagt tcaagcatga tgactcctga aaaaacaaaa tcgtgcctca
     3421 caccttaatg cgattagccg atggcggaac gacaaatttt gttaattttt atgagttcgg
     3481 gcaacacctt tatagcaaac tggcgtagag tctgatttca ggcccgatat aaagtggtaa
     3541 cgaggcgttc cagcgcgggc caggctctta cggtgcggca gaatttccag tataatagcc
     3601 gccttttttc attcagttgt gacatacagc taacgctgcg acttggtcac ctgcgcagca
     3661 gggggcatcc gccggcagct aagttaaggg atatctcatg cgtacagaat attgtggaca
     3721 gctccgtttg tcccacgtgg ggcagcaggt gactctgtgt ggttgggtca accgtcgtcg
     3781 tgatcttggt agcctgatct tcatcgatat gcgcgaccgc gaaggtatcg tgcaggtatt
     3841 tttcgatccg gatcgtgcgg acgcgttaaa gctggcctct gaactgcgta atgagttctg
     3901 cattcaggtc acgggcaccg tacgtgcgcg tgacgaaaaa aatattaacc gcgatatggc
     3961 gaccggcgaa atcgaagtgc tggcgtcctc gctgactatc atcaatcgcg cagatgttct
     4021 gccgcttgac tctaaccacg tcaacaccga agaagcgcgt ctgaaatacc gctacctcga
     4081 cctgcgtcgc ccggaaatgg ctcagcgcct gaaaacccgc gctaaaatca ccagcctggt
     4141 gcgccgtttt atggatgacc acggcttcct cgatatcgaa actccgatgc tgaccaaagc
     4201 cacgccggaa ggcgcgcgtg actacctggt gccttctcgt gtgcacaaag gtaaattcta
     4261 cgcactgccg caatccccac agttgttcaa acagctgctg atgatgtccg gctttgaccg
     4321 ctactatcag atcgttaaat gcttccgtga cgaagacctg cgcgctgatc gtcagcctga
     4381 atttactcag atcgatgtgg aaacttcttt catgaccgcg ccgcaagtgc gtgaagtgat
     4441 ggaagcgctg gtgcgccatc tgtggctgga agtgaagggc gtggatctgg gcgatttccc
     4501 ggtaatgacc tttgcggaag ctgaacgccg ttacggttcc gataaaccag atttgcgtaa
     4561 cccgatggaa ctgaccgacg ttgccgatct gctgaaatct gttgagtttg cagtatttgc
     4621 aggtccggcg aacgatccga aaggtcgcgt tgccgctctg cgtgttccgg gtggcgcatc
     4681 gctgacccgt aagcagatcg acgaatacgg taacttcgtt aaaatctacg gcgcgaaagg
     4741 tctggcttac atcaaagtta acgaacgcgc gaaaggtctg gaaggtatca acagcccggt
     4801 agcgaagttc cttaatgcag aaatcatcga agccatcctg gatcgtactg ccgcgcaaga
     4861 tggcgatatg attttcttcg gtgccgacaa caagaaaatt gttgccgacg cgatgggcgc
     4921 actgcgcctg aaagtgggta aagaccttgg tctgaccgac gaaagcaaat gggcaccgct
     4981 gtgggttatc gacttcccga tgtttgaaga cgacggtgaa ggcggcctga cggcaatgca
     5041 ccatccgttc acctcaccga aagacatgac ggctgcagag ctgaaagctg caccggaaaa
     5101 tgcggtggcg aacgcttacg atatggtcat caatggttac gaagtgggcg gtggttcagt
     5161 acgtatccat aatggtgata tgcagcagac ggtgtttggt attctgggta tcaacgaaga
     5221 ggaacagcgc gagaaattcg gcttcctgct cgacgctctg aaatacggta ctccgccgca
     5281 cgcaggtctg gcattcggtc ttgaccgtct gaccatgctg ctgaccggca ccgacaatat
     5341 ccgtgacgtt atcgccttcc cgaaaaccac ggcggcagcg tgtctgatga ctgaagcacc
     5401 gagctttgct aacccgactg cactggctga gctgagcatt caggttgtga agaaggctga
     5461 gaataactga tatgagtcaa atacacgaaa tcattcgcgt tgcatcgagg cggcaactga
     5521 gtgaactccc atgagcatag ataactatgt gaataggatg agcgaaggca gtcaacgaag
     5581 aggcagcgtg aaggataaag tgtataagcg tcccgtttcg atcttagtgg tcatctacgc
     5641 acaagatacg aaacgggtgc tgatgttgca gcggcgtgat gaccccgatt tctggcagtc
     5701 ggtaaccggc agcgtggaag agggtgaaac cgcgccgcaa gctgccatgc gcgaagtaaa
     5761 ggaagaggtc accattgatg ttgtcgctga acaactgacc ttaattgact gtcagcgcac
     5821 ggttgagttt gaaatttttt cacatttacg tcatcgctat gcgccgggcg tgacgcgtaa
     5881 tacggaatca tggttctgtc ttgcgcttcc gcacgagcgg cagatcgttt tcactgaaca
     5941 tctggcttac aagtggcttg atgcgcctgc tgcggcggcg ctcactaagt cctggagcaa
     6001 ccggcaggcg attgaacagt ttgtaattaa cgctgcctga acaggcaggg ctatctggag
     6061 atatttttat ggcaggtcat agtaaatggg ccaacaccag acatcgtaaa gctgcgcagg
     6121 atgctaagcg cggtaaaatc ttcactaaaa tcattcgtga gctggtaacc gcggctaagc
     6181 tgggcggtgg cgatccggac gctaacccgc gtctgcgtgc agcggtggat aaagcactgt
     6241 ctaacaacat gacccgtgac actctgaacc gcgcaattgc acgtggtgtg ggcggtgatg
     6301 atgatgcgaa catggaaacc atcatctacg aaggttacgg tcctggcggc acggcaatca
     6361 tgattgaatg tctgtctgac aaccgcaacc gtaccgttgc tgaagtgcgt catgcattca
     6421 gcaaatgtgg cggtaacctc ggtactgatg gttccgtagc ctatctgttc agcaaaaaag
     6481 gcgtcatctc cttcgagaaa ggcgatgaag acaccatcat ggaagcagca ctggaagcag
     6541 gtgctgaaga cgttgtgacc tatgatgacg gcgcgattga tgtctacact gcatgggaag
     6601 aaatgggtaa agtgcgcgac gctctggaag cggcaggtct gaaagcagac agcgcggaag
     6661 tttccatgat cccgtctacc aaagctgata tggatgcaga aaccgcaccg aaactgatgc
     6721 gtctgatcga tatgctggaa gattgcgacg acgtgcagga agtttaccat aacggtgaaa
     6781 tctctgatga ggtcgcagcg actctctgat gaggcctgct aaacagcaaa acggagacgc
     6841 gtgatggcta ttattctcgg cattgatccg ggttcgcgcg tgaccggcta cggcgtcatc
     6901 cgccaggtag gtaggcaact gtcctacctg ggtagcgggt gcatccgcac caaagtggat
     6961 gatttaccgt ctcgtctgaa actcatctat gcgggcgtga cggaaatcat cacccagttc
     7021 cagcctgatt atttcgccat tgaacaagtc tttatggcaa agaacgctga ctcagccctg
     7081 aaactgggcc aggcgcgcgg cgtggcgatt gtggcggcgg tgaatcagga gttgccagta
     7141 tttgaatacg cggcacgtca ggtaaagcaa acggtggtag gtattggtag tgccgaaaaa
     7201 agccaggtgc agcatatggt ccgcaccttg ctgaaactgc ccgctaatcc acaggcggat
     7261 gccgccgatg cgctggcgat tgccatcacc cactgccacg ttagtcagaa tgcgatgcag
     7321 atgagcgaat cgcggctgaa cctggcgaga gggcgactgc gttaagttat accgcctcgg
     7381 tcagttccgg ctgaggcgtt tccactccct ccgcatgaat caacaccaaa cccggatgat
     7441 gccacaaact ggctggcgtg acggttttac gctcccacgg aatagaaccc aggaaccaga
     7501 atttccagaa agtgagtttt gcattcggat tgctatttaa caattctcca aacgtctcta
     7561 tttcacccac cggaatacat agcgcttctt tataaatatc aaaaacaaat tttgaacaga
     7621 actggcgcga agattcgtat ttaaaaccgg tgtggtaaag tttgcgtagc cgggagggaa
     7681 cctgttcaac aattcgttgt ttttgttgtt ctgtcagccc agcgtctaat cgctttatag
     7741 catagcgttg attagacgaa cgtttaataa aacgagatag cgtggtgatg gttgaaaggg
     7801 gaacccggct ttctgcaacc aggaagtctt caccgttatg accgataatg atcccgacgt
     7861 gattactcca gcaatttgat gcagctgata tttgaccaaa taaggcagca cctatacatg
     7921 taaagacgat atctccaatt tcatattcag cagggtaatt aatattcacg ttgtcattcc
     7981 attgaaatag atacgaatat acgtaatagc ataaaaaatt tattttttca ggaggcgcaa
     8041 tgtatatttt tattacgcat ttattcactg aatatgtaat attaaaatat ttgcttccaa
     8101 tataacctgt agaataaatt atactgtgcc atttttcagt tcatcgagac acctcgcaag
     8161 ttttcttcat ccttcgctgg atatctatcc agcatttttt tatcatacag cattatcttt
     8221 gattcattac gcaggagcgt catgtgatag gcagactcag aggcatcatc attgaaaaac
     8281 aacccccgct ggtgttaatt gaagtgggcg gcgtaggcta tgaagtgcat atgccgatga
     8341 cctgttttta tgaactccct gaagcgggtc aggaagcgat cgtcttcacc cactttgtgg
     8401 tgcgtgaaga cgcgcaactg ctgtacggtt ttaacaataa acaagagcgc acattgttta
     8461 aagagttgat caaaaccaac ggcgtcggcc cgaagttggc gctggcgatc ctctccggaa
     8521 tgtcagcgca gcagttcgtt aatgccgttg agcgtgaaga agtgggggca ctggtgaaac
     8581 tgccgggtat tggcaaaaaa accgccgaac gcttgattgt tgaaatgaaa gaccgattta
     8641 aaggtttgca tggcgatctc tttacgccag ccgccgacct ggtactcacg tctcctgcca
     8701 gcccggcgac cgacgatgct gaacaagaag cggttgccgc gctggtggcg ctgggctata
     8761 aaccacaaga agcaagccgc atggtgagca aaatcgctcg ccctgacgcc agcagtgaaa
     8821 cattaattcg cgaagcccta cgcgccgcgt tatgaggtaa aggatgattg aagcagaccg
     8881 tctgatttct gccggtacca ctttgccgga agatgtagca gatcgcgcca ttcgccctaa
     8941 attactggaa gagtatgttg gtcagccgca ggttcgttca cagatggaga ttttcatcaa
     9001 agcagcgaaa ctgcgcggcg atgccctcga tcatttgttg atttttggtc ctccggggtt
     9061 gggtaaaact acgcttgcca acattgtcgc caatgaaatg ggcgttaatt tacgcacgac
     9121 ttctggtccg gtgctggaaa aggcgggcga tttggctgcg atgctcacta accttgaacc
     9181 gcatgacgtg ctgtttattg atgagatcca ccgtttatcg ccagttgttg aagaagtgct
     9241 gtacccggca atggaagact accaactgga tatcatgatt ggtgaaggtc cggcggcacg
     9301 ctccattaaa attgatttgc cgccgtttac cctgattggt gcaaccacgc gcgcaggttc
     9361 gctgacatca ccgttgcgcg accgttttgg tattgtgcaa cgtctggagt tttatcaggt
     9421 gccggatctg caatatatcg tcagtcgcag cgcacgcttt atggggcttg agatgagtga
     9481 tgacggcgcg ctggaagttg ctcgtcgcgc tcgcggtacg ccgcgcattg ccaaccgtct
     9541 gctgcgtcga gtgcgtgatt tcgccgaagt gaagcacgat ggcaccatct cggcagatat
     9601 cgctgctcag gcgctggata tgttgaatgt cgatgctgaa ggtttcgatt atatggaccg
     9661 caaattgttg ctggcggtaa tcgataagtt ctttggtggg ccggtaggtc tggataacct
     9721 ggcggcggct attggcgaag aacgtgaaac cattgaggat gtgctggaac cttatttgat
     9781 tcagcaaggc tttttgcagc gtacaccgcg cgggcgtatg gcgacgacgc gggcgtggaa
     9841 tcactttggc ataacgccgc cagaaatgcc gtaaatcgga ttggtcgctc gcaccgcatc
     9901 cgacattggc agtgtgcctg atgcgacgct attcgcgtct tatcaggcat ataccagaga
     9961 tagcgaaccg tagggcggaa aaggcgctcg cgccgctgtt cgcgtcttac caggcctacc
    10021 agagatagcg aaccgtaggt cggataaggc gctcgcgccg catccgacaa atgtgttcag
    10081 cgatagatta gctggcctgc tttttcatca tactgataat aaataacagt gccgcacata
    10141 gcaccaccga cggacccgcc ggcgtatcgt aaaacgcgga aaaggttaaa ccgccagtta
    10201 ctgccaccat ccccaccaaa acagcgacac cagccatctg ttccggcgta cgggcaaagc
    10261 gacgcgcagt agctgcagga ataatcagca acgaagtaat aatcaacgca ccgacaaatt
    10321 tcatcgctac accaatcgtc aatgccgtca ccagcatcaa caacaatttc actcgctgta
    10381 atttcacacc atcaacaaac gccagatccg ggctaatcgt catcgacagc aaattgcgcc
    10441 attgccagaa caaaatagcc accacgatga ccacgccaat tgcaatagag atgagatctt
    10501 ctggcgtcac tgccagtaaa tcaccgaaca ggtaagccat caaatcaaca cgaatgttag
    10561 acatcagact aacgaccacc aggcccagcg acagggcact gtgcgccata atccctaata
    10621 acgtgtcgat cgccagctgt ggacgcttct ccagccatac cagaccgccc gccagcagca
    10681 gcgtaacggc aatcaccgca tagaatggat tcacgtccag caacaaacca aacgcgacac
    10741 caagtaatga ggcatgagcc agcgtatcac cgaaataaga catacgacgc cagactacaa
    10801 acgaacccag cggacccgcg gcacaggcga gcatgatccc ggctaaccaa ccgggaaata
    10861 ataattcaat catgagcgat catttccccg acgcaaaaca attcgtccct gtaaatcgtg
    10921 acgatgatta tgatgatggc gatagatacc cagttgttca gcaccacgag gaccaaacat
    10981 tgaaataaac tccggatgca gggaaacaac ttccggtgtg ccggaacaac aaatgtggtg
    11041 attcaggcac agcacttcat cggtttttgc catcaccaga tgcagatcgt gagaaaccat
    11101 taaaacgcca caatccagtt cacgacgcag ttggtcaata aggtcatata acgccacctg
    11161 gccatttaca tccacgcctt gagtgggttc atccagcact aataattgcg gtcgatttaa
    11221 caatgctcgc gctaacagta cacgctgcgt ttcgccacca gagagctttt gcatcggtgc
    11281 gttaatcagg tgcccggcct ggacacgttt cagtgcaggc aaaatatctt ctttatgtgt
    11341 accagggcgt aagcgtaaaa aacggtttac ggtcagtggc aacgtggtgt cgagatacag
    11401 cttctgcggt acatagccga tgcgcagttt tccgttgcgc ttgataaccc cttcatcggg
    11461 tgttaccagc ccgagcacta cccgtaccag tgtcgactta cctgcgccat ttggcccaag
    11521 taaagtcaaa atttttccag gtttaagttc cagcgacaca tcagagagga cgcggcgttg
    11581 gccaaaagaa accgagacat tttccaggga aaccagactt gtcatgttaa ttttagtctt
    11641 gcagtagtca tgaaatgtta taatatcaca cttctcatat tcattacgat gattggtcgc
    11701 attatgttac ataaaaaaac gcttcttttc gcagcattat ccgccgctct ctggggaggt
    11761 gcaacacagg ccgcagatgc cgccgttgtc gcttcgctta aacccgttgg gttcatcgct
    11821 tctgccattg ctgatggggt aacagaaacg gaggttttac tgcctgacgg tgcttcagaa
    11881 catgattatt cactgcgtcc atcggatgta aaacgcttac agaacgcgga cttagtcgtt
    11941 tgggttggcc cggagatgga agcgtttatg caaaaaccgg taagcaaatt accagaagcg
    12001 aagcaggtaa cgattgcgca gctagaagat gtgaaaccgc tactgatgaa aagtattcac
    12061 ggcgatgatg atgatcacga ccacgcggaa aaaagtgacg aagatcacca tcacggcgat
    12121 ttcaacatgc atctttggct ttccccagag atagcgcggg ctacagcggt tgcaatccat
    12181 ggaaaattag tggaacttat gccgcaaagt cgagccaaac ttgacgccaa cctgaaggat
    12241 tttgaggcac aattagcctc aaccgaaacg caggttggta acgagctcgc gccgctcaag
    12301 gggaaaggtt atttcgtttt tcacgatgct tacggctatt tcgaaaaaca gttcggactg
    12361 acaccgcttg gtcattttac cgttaaccct gagattcaac ctggcgcgca gcgtttacat
    12421 gaaataagaa cacagttggt tgagcaaaaa gcaacctgcg tttttgctga gccacagttc
    12481 aggccagcgg tcgttgagag cgtcgcacga gggacatccg ttcgtatggg aacgttggat
    12541 cccctgggga cgaatatcaa actgggtaaa acaagctatt cagaattcct gagtcaatta
    12601 gccaaccagt atgcgagctg cctgaaagga gattaatgag gaagtgatta cgtgcaacag
    12661 atagcccgct ctgtcgccct ggcgtttaat aatttaccgc gaccacaccg cgttatgttg
    12721 gggtcgctca ccgttcttac tctggccgtc gctgtctggc ggccctatgt ttatcaccgc
    12781 gatgccacgc caattgtcaa aaccattgag ctggaacaga acgaaattcg ttcgctctta
    12841 cctgaagcca gtgagccgat tgatcaagct gcacaagaag atgaagccat tccccaggac
    12901 gaactggatg acaaaatcgc cggtgaagcg ggcgtgcatg aatatgttgt ttccactggc
    12961 gatacgctaa gcagcattct caatcagtat ggtattgata tgggtgatat cacccaactg
    13021 gctgcggccg acaaagaatt gcgtaacctg aaaatcggtc aacaactctc ctggacatta
    13081 accgcggacg gcgaactgca gcgcctcacc tgggaagtgt ctcgtcgtga aacccgaacc
    13141 tatgaccgta ctgccgctaa cggttttaaa atgaccagcg aaatgcagca aggagagtgg
    13201 gttaacaatc tgctgaaagg taccgtcggg gggagctttg ttgccagcgc cagaaacgcc
    13261 ggtttaacca gcgccgaagt gagcgcagtg attaaagcca tgcagtggca aatggatttc
    13321 cgcaaactga aaaaaggcga tgaatttgcg gtattaatgt ctcgagaaat gcttgatggt
    13381 aaacgtgagc aaagccagct gctgggcgta cgtttgcgtt cagaaggtaa agattattac
    13441 gctatccgcg ctgaagatgg caaattctac gatcgtaacg gtactggtct ggcgaaagga
    13501 ttcttgcgat tcccgacggc gaaacagttc cgtatctcgt ctaactttaa cccgcgtcgt
    13561 actaatccgg tgaccggtcg cgttgcacca cacagaggtg ttgatttcgc catgccgcag
    13621 ggtacgccag tgctttcagt gggtgacggt gaagtggtgg ttgccaaacg cagtggcgca
    13681 gcaggttatt atgtggctat tcgtcatggt cgcagctaca ccacgcgtta tatgcacttg
    13741 cgcaagattc tggtgaaacc gggacagaag gtgaaacgtg gcgaccgtat cgcgctttcc
    13801 ggtaataccg gacgttcaac cgggccgcat ctgcactatg aagtatggat aaaccagcag
    13861 gccgtaaacc cgctgacggc aaaactgccg cgtaccgaag ggctgaccgg ctccgatcgt
    13921 cgcgaattcc tggcgcaggc caaagagatt gtgccgcagc tacggtttga ttaattaaca
    13981 tccattcgca gccggtacgt agtcagtacc ggcttttttt atttggtgcg gggcaagttg
    14041 cgccgctaca ctatcaccag attgattttt gccttatccg aaactggaaa agcatggaaa
    14101 cgaaaaaaaa taatagcgaa tacattcctg agtttgataa atcctttcgc cacccgcgct
    14161 actggggagc atggctgggc gtagcagcga tggcgggtat cgctttaacg ccgccaaagt
    14221 tccgtgatcc cattctggca cggctgggac gttttgccgg acgactggga aaaagctcac
    14281 gccgtcgtgc gttaatcaat ctgtcgctct gctttccaga acgtagtgaa gctgaacgcg
    14341 aagcgattgt tgatgagatg tttgccaccg cgccgcaagc gatggcaatg atggctgagt
    14401 tggcaatacg cgggccggag aaaattcagc cgcgcgttga ctggcaaggg ctggagatca
    14461 tcgaagagat gcggcgtaat aacgagaaag ttatctttct ggtgccgcac ggttgggccg
    14521 tcgatattcc tgccatgctg atggcctcgc aagggcagaa aatggcagcg atgttccata
    14581 atcagggcaa cccggttttt gattatgtct ggaacacggt gcgtcgtcgc tttggcggtc
    14641 gtctgcatgc gagaaatgac ggtattaaac cattcatcca gtcggtacgt caggggtact
    14701 ggggatatta tttacccgat caggatcatg gcccagagca cagcgaattt gtggatttct
    14761 ttgccaccta taaagcgacg ttgcccgcga ttggtcgttt gatgaaagtg tgccgtgcgc
    14821 gcgttgtacc gctgtttccg atttatgatg gcaagacgca tcgtctgatg attcaggtgc
    14881 gcccaccgat ggatgatctg ttagaggcgg atgatcatac gattgcgcgg cggatgaatg
    14941 aagaagtcga gatttttgtt ggtccgcgac cagaacaata cacctggata ctaaaattgc
    15001 tgaaaactcg caaaccgggc gaaatccagc cgtataagcg caaagatctt tatcccatca
    15061 aataaaaaag cctctcgcga ggagaggcct tcgcctgatg ataagttcaa gtttgcttca
    15121 gaatattcga aatctgttga actatcattg aactgtaggc cggatgtggc gttttcgccg
    15181 catccggcaa cgtacttact ctaccgttaa aatacgcgtg gtattagtag aacccacggt
    15241 actcatcacg tcgccctggg tgacaatcac caggtcacca gacatcaagt aacctttatc
    15301 gcgcagcaga ttaaccgctt cgctggcagc tgctacgccg tcattagcgc tatcaaagtg
    15361 caccggcgta acgccacgat agagagcagt caggttcagc gtacgttcat ggcgcgacat
    15421 ggcgaaaatt ggtagaccag agctgatacg ggaggtcatc agcgcggtac gacccgattc
    15481 ggtcatggta atgatcgccg taacgccttt caggtggtta gctgcgtaca ttgctgacat
    15541 ggcaatagct tcttccacat tgtcgaactg aacgtccaga cggtgtttag aaacgttgat
    15601 gctcgggatt ttttccgcac ccaggcaaac gcgcgccatg gctgcaacag tttctgacgg
    15661 atactgccca gcggcagttt ctgcagacag catcacagcg tcagtaccat ccagaacggc
    15721 gtttgctacg tccatgactt ctgcacgcgt cggcatcggg ttagtaatca ttgactccat
    15781 catctgggtc gccgtgatta ccgcgcggtt aagctgacgt gcacgacgga tcaacgcttt
    15841 ctgaatgccg accagttccg ggtcgccaat ttccacaccg aggtcgccac gtgcaaccat
    15901 taccacgtca gaggcgagga tgatgtcatc cattgcatcc tggctgcaaa cggcttccgc
    15961 acgttcaacc ttggcaacaa ttttcgcatc acatcctgca tcgcgtgcca ggcgacgggc
    16021 atagttcaga tcttcgccac agcgtgggaa ggagacagcc aggtaatcta cgccaatcaa
    16081 cgccgcagtc ttaatgtctg ctttgtcttt ttcggtcagc gcttcagccg acaaaccgcc
    16141 gccaagtttg ttgatacctt tattgttgga gaggggacca ccgacggtga cttcggtgaa
    16201 cactttcatg ccctgaactt ccagtacttt taactggacg cgaccatcgt ccagcagcag
    16261 gatgtcgcca ggcacaacgt cagcaggcag gcctttgtag tcgataccga ctttttcttt
    16321 gtcgccttca cctttaccca ggttggcgtc gagcaggaat ttatccccaa tattgaggaa
    16381 aactttgcct tctttaaagg tggatacacg gattttgggc ccctggaggt cacccagaat
    16441 agccacatga cgccccagtt ttgcggcaat ctcacgaact ttatccgcgc gcattttgtg
    16501 atcttcaggc gagccgtgag aaaagttcat acgtacaacg ttggcacccg ccgcgataac
    16561 tttttcaagg ttattatcgc gatctgttgc tgggcctaac gtggtaacga tttttgttct
    16621 gcgaagcctt ctggacatgt aatactccgt tgactgaaac aaccaggtgt tgcttgaaca
    16681 tgaaatccga atgaaataac gccgcgatgg cgataaatta atctcattcg gatgtcgtat
    16741 aagattagga cagtgacagt cgcttttagc gatcgtcact taaattaagt aactgcttat
    16801 caaaacgcga ttccttcagc gcttccttga cccgcttcaa gttatctctg aattttgcac
    16861 cgcgtcgcaa agtaaatcct gtcgccagca catctatcac ggtcagctgt gcaagtcgag
    16921 aaaccatggg catataaatg tcagtatctt ccggtacgtc gagggtaatt gccagcgttg
    16981 cttcccgggc gagcggggta cccgcagagg tgagggcaat caccatagcg tcgttttcgc
    17041 gtgccagctg cgccagctcg accagatttt ttgttcttcc agtgtgagaa atcagcacca
    17101 ccacgtctcc gtcgctacaa ttcatacaac tcatgcgttg cagcacgata tcatcggagt
    17161 acaccaccgg aacattaaaa cgaaagaact tattcatcgc atcgtgggca acggcggctg
    17221 aagagcctaa tccgaaaaag gcgatttttt ttgcctgagt gagcaagtcg acggcgcggt
    17281 tgatggcaga tttatccagt gaatgacgga catgatcgag cgttgccatt gcggactcaa
    17341 atattttccc cgtgtatgat tcaacgctgt catcttcatt gacgttgcga ttaacatagg
    17401 gagtgccatt cgccagactt tgtgccagat gaagtttaaa atcaggaaaa ccgcgcgtgt
    17461 ccatgctgcg acagaaacga ttcaccgtcg gttcgctaac attggcttcc agtgccagag
    17521 cagcaatact cgaatggatc gcgttatcgg gcgaagccag aatgacctcg gcaactttgc
    17581 gctctgattt gctcaaatgt tccagctgag actggatttt ttccagcata ttcatgatgt
    17641 aaagagactc acgggtaatg acgatttccg cactgaaaga aatcgaaatg cagttttgtc
    17701 agatattacg cctgtgtgct gtgttaatga caaaagcaga taaaaaagtt gttatttttt
    17761 ttcataacat gatcagtgtc agatttttac ccaatggaaa acgatgattt ttttatcagt
    17821 tttgccgcac tttgcgcgct tttaccgtaa tcgcacgggt ggataagcgt ttacagtttt
    17881 cgcaagctcg taaaagcagt acagtgcacc gtaagaaaat tacaagtata ccctggctta
    17941 agtaccgggt tagttaactt aaggagaatg acatggcggt aacgcaaaca gcccaggcct
    18001 gtgacctggt cattttcggc gcgaaaggcg accttgcgcg tcgtaaattg ctgccttccc
    18061 tgtatcaact ggaaaaagcc ggtcagctca acccggacac ccggattatc ggcgtagggc
    18121 gtgctgactg ggataaagcg gcatatacca aagttgtccg cgaggcgctc gaaactttca
    18181 tgaaagaaac cattgatgaa ggtttatggg acaccctgag tgcacgtctg gatttttgta
    18241 atctcgatgt caatgacact gctgcattca accgtctcgg tgcgatgctg gatcaaaaaa
    18301 atcgtatcac cattaactac tttgccatgc cgcccagcac ttttggcgca atttgcaaag
    18361 ggcttggcga ggcaaaactg aatgctaaac cggcacgcgt agtcatggag aaaccgctgg
    18421 ggacgtcgct ggcgacctcg caggaaatca atgatcaggt tggcgaatac ttcgaggagt
    18481 gccaggttta ccgtatcgac cactatcttg gtaaagaaac ggtgctgaac ctgttggcgc
    18541 tgcgttttgc taactccctg tttgtgaata actgggacaa tcgcaccatt gatcatgttg
    18601 agattaccgt ggcagaagaa gtggggatcg aagggcgctg gggctatttt gataaagccg
    18661 gtcagatgcg cgacatgatc cagaaccacc tgctgcaaat tctttgcatg attgcgatgt
    18721 ctccgccgtc tgacctgagc gcagacagca tccgcgatga aaaagtgaaa gtactgaagt
    18781 ctctgcgccg catcgaccgc tccaacgtac gcgaaaaaac cgtacgcggg caatatactg
    18841 cgggcttcgc ccagggcaaa aaagtgccgg gatatctgga agaagagggc gcgaacaaga
    18901 gcagcaatac agaaaccttc gtggcgatcc gcgtcgacat tgataactgg cgctgggccg
    18961 gtgtgccatt ctacctgcgt actggtaaac gtctgccgac caaatgttct gaagtcgtgg
    19021 tctatttcaa aacacctgaa ctgaatctgt ttaaagagtc gtggcaggat ctgccgcaga
    19081 ataaactgac tatccgtctg caacctgatg aaggcgtgga tatccaggta ctgaataaag
    19141 ttcctggcct tgaccacaaa cataacctgc aaatcaccaa gctggatctg agctattcag
    19201 aaacctttaa tcagacgcat ctggcggatg cctatgaacg tttgctgctg gaaaccatgc
    19261 gtggtattca ggcactgttt gtacgtcgcg acgaagtgga agaagcctgg aaatgggtag
    19321 actccattac tgaggcgtgg gcgatggaca atgatgcgcc gaaaccgtat caggccggaa
    19381 cctggggacc cgttgcctcg gtggcgatga ttacccgtga tggtcgttcc tggaatgagt
    19441 ttgagtaata tctgcgctta tcctttatgg ttattttacc ggtaacatga tcttgcacag
    19501 attgtagaac aatttttaca ctttcaggcc tcgtgcggat tcacccacga ggcttttttt
    19561 attacactga ctgaaacgtt tttgccctat gagctccggt tacaggcgtt tcactcataa
    19621 atcctctgaa tgaaacgcat tgtgaatcat cctgctctga caactcattt tcaggagcct
    19681 ttatgaatcc acaattgtta cgcgtaacaa atcgaatcat tgaacgttcg cgcgagactc
    19741 gctctgctta tctcgcccgg atagaacaag cgaaaacgtc gaccgttcat cgttcgcagt
    19801 tggcatgcgg taacctggca cacggtttcg ctgcctgcca gccagaagac aaagcctctt
    19861 tgaaaagcat gttgcgtaac aatatcgcca tcatcacctc ctataacgac atgctctccg
    19921 ctcaccagcc ttatgaacac tatccagaaa tcattcgtaa agccctgcat gaagcgaatg
    19981 cggttggtca ggttgcgggc ggtgttccgg cgatgtgtga tggtgtcacc caggggcagg
    20041 atggaatgga gttgtcgctg ctaagccgcg aagtgatagc gatgtctgcg gcggtcgggc
    20101 tgtcccataa catgtttgat ggtgctctgt tcctcggggt gtgcgacaag attgtcccgg
    20161 gtctgacgat ggcagccctg tcgtttggtc atttgcctgc ggtgtttgtg ccgtctggac
    20221 cgatggcaag cggtttgcca aataaagaaa aagtgcgtat tcgccagctt tatgccgaag
    20281 gtaaagtgga ccgcatggcc ttactggagt cagaagccgc gtcttaccat gcgccgggaa
    20341 catgtacttt ctacggtact gccaacacca accagatggt ggtggagttt atggggatgc
    20401 agttgccagg ctcttctttt gttcatccgg attctccgct gcgcgatgct ttgaccgctg
    20461 ccgctgcgcg tcaggttaca cgcatgaccg gtaatggtaa tgaatggatg ccgatcggta
    20521 agatgatcga tgagaaagtg gtggtgaacg gtatcgttgc actgctggcg accggtggtt
    20581 ccactaacca caccatgcac ctggtggcga tggcgcgcgc ggccggtatt cagattaact
    20641 gggatgactt ctctgacctt tctgatgttg taccgctgat ggctcgtctc tacccgaacg
    20701 gtccggccga tattaaccac ttccaggcgg caggtggcgt accggttctg gtgcgtgaac
    20761 tgctcaaagc aggcctgctg catgaagatg tcaatacggt ggcaggcttt ggtctgtctc
    20821 gttataccct tgaaccatgg ctgaataatg gtgaactgga ctggcgggaa ggggcggaaa
    20881 aatcactcga cagcaatgtg atcgcttcct tcgaacaacc tttctctcat catggtggga
    20941 caaaagtgtt aagcggtaac ctgggccgtg cggttatgaa aacctctgcc gtgccggttg
    21001 agaaccaggt gattgaagcg ccagcggttg tttttgaaag ccagcatgac gttatgccgg
    21061 cctttgaagc gggtttgctg gaccgcgatt gtgtcgttgt tgtccgtcat caggggccaa
    21121 aagcgaacgg aatgccagaa ttacataaac tcatgccgcc acttggtgta ttattggacc
    21181 ggtgtttcaa aattgcgtta gttaccgatg gacgactctc cggcgcttca ggtaaagtgc
    21241 cgtcagctat ccacgtaaca ccagaagcct acgatggcgg gctgctggca aaagtgcgcg
    21301 acggggacat cattcgtgtg aatggacaga caggcgaact gacgctgctg gtagacgaag
    21361 cggaactggc tgctcgcgaa ccgcacattc ctgacctgag cgcgtcacgc gtggggacag
    21421 gacgtgaatt attcagcgcc ttgcgtgaaa aactgtccgg tgccgaacag ggcgcaacct
    21481 gtatcacttt ttaagacgac aaatttgtaa tcaggcgaga gaaaactctg atgaaaaact
    21541 ggaaaacaag tgcagaatca atcctgacca ccggcccggt tgtaccggtt atcgtggtaa
    21601 aaaaactgga acacgcggtg ccgatggcaa aagcgttggt tgctggtggg gtgcgcgttc
    21661 tggaagtgac tctgcgtacc gagtgtgcag ttgacgctat ccgtgctatc gccaaagaag
    21721 tgcctgaagc gattgtgggt gccggtacgg tgctgaatcc acagcagctg gcagaagtca
    21781 ccgaagcggg tgcacagttc gcaattagcc cgggtctgac cgagccgctg ctgaaagctg
    21841 ctaccgaagg gactattcct ctgattccgg ggatcagcac tgtttccgaa ctgatgctgg
    21901 gtatggacta cggtttgaaa gagttcaaat ttttcccggc tgaagctaac ggcggcgtga
    21961 aagccctgca ggcgatcgcg ggtccgttct cccaggtccg tttctgcccg acgggtggta
    22021 tttctccggc taactaccgt gactacctgg cgctgaaaag cgtgctgtgc atcggtggtt
    22081 cctggctggt tccggcagat gcgctggaag cgggcgatta cgaccgcatt actaagctgg
    22141 cgcgtgaagc tgtagaaggc gctaagctgt aaaagtcaaa atgcccgatc gaggatcggg
    22201 catttttgta gcgttttttt gccgggttta accctgtact tttacctgtc cggcggcgtg
    22261 cttcgcgcgt tcaatggcgt caacaacact ctctgcagta gccagtgcca cccccagacg
    22321 acggctgcca tcaatttccg gcttaccaaa taaacgaatc tgcaaatctg cgcctacggc
    22381 attctgcaca ttatcaaacg tgacattctg actggtcagt tgtggcagaa taacggcaga
    22441 agctgcagga ccatactgac ggatcccgcc aaccggaagt ccgaggaagg cacgtacatg
    22501 cagggcaaac tctgagagat cttgagaaat taacgtcacc atcccggtat catgtggacg
    22561 aggggagacc tcactgaaaa tcacctcatc accacagaca aacagctcga caccaaacag
    22621 accataaccg cccagtgcca gcaccacttt acgggcaatc tcctgcgcac gttcaagggc
    22681 aagcgggctc atttgctgtg gttgccagga ttcacggtag tcgccatctt cctggcgatg
    22741 acctactggt gcacagaaat ggacgccatc caccgcgctg acggttagca gggtaatttc
    22801 gaagtcaaac ttaacgacgc cttcaacaat tacgcggccc gctccggcgc gaccgccttg
    22861 ctgagcgtat tcccatgcct gagcaagttg ctctgcagag cgaataaacg tctgcccctt
    22921 gccggaagag ctcatcaccg gttttacaat gcagggatag ccaatggcgg caaccgcctc
    22981 tcggaaaagg ctttcgctat cggcaaaacg ataagtggaa gtgggcagct gcagctcttc
    23041 tgccgccagg cgacggatac cctcgcgatt catcgttaat ttcgttgcgc gagcgcaggg
    23101 gacaacattc agtccttcct cttcaagttg gatcagcata tcggtggcaa tagcttcgat
    23161 ctccggcacg atataatgtg gtttttccag ttcaaccaca cggcgtaatg catcaccatc
    23221 aagcatatta atgacatggg agcgatgcgc gacatgcatg gctggtgcgt cggcatagcg
    23281 atcgacggca atcacctcta cgccgagacg ctgacactcg attgccactt ctttacccag
    23341 ttcaccggag cctaataaca tcacgcgagt tgctgctgga cgcagcgcag tgcctaataa
    23401 cgtcataacg aatatcccct gattaattcc gcgcgcagta taaacgaaaa cgtttgcgtg
    23461 tgtctttatt gctggtgttg atttctcaac cgaaaagaaa tatactgtat aaaatcacag
    23521 ttattatgag aggctgcaaa tggcggttga agttaaatac gtagtcattc gtgagggcga
    23581 agagaaaatg tcgtttacca gcaaaaagga agccgatgct tatgacaaaa tgcttgatac
    23641 ggcggatctg cttgacacct ggctgacaaa ttctccagtg caaatggaag acgagcaacg
    23701 tgaagccctt tcgctatggc tggcagaaca aaaagatgtg ctgagcacca ttctgaaaac
    23761 cggcaaatta ccgtccccac aggtcgtcgg tgctgaatcc gaagaggaag atgcctcaca
    23821 cgcggcctga agcaacttgc gctcctgaca atttgtacca tgctttctcc tgaatcattg
    23881 tgctatggag aaaaacatga aaaaaagagg ggcgttttta gggctgttgt tggtttctgt
    23941 ctgcgcatca gttttcgctg ccaataatga aaccagcaag tcggtcactt tcccaaagtg
    24001 tgaaggtctg gatgctgccg gaattgccgc gagcgtaaaa cgtgattatc aacaaaatcg
    24061 cgtggcgcgc tgggcagatg atcaaaaaat tgtcggtcag gccgatcccg tggtatgggt
    24121 cagtttgcag gacattcagg gtaaagatga taaatggtca gtaccgctaa ccgtacgtgg
    24181 taaaagtgcc gatattcatt accaggtcag cgtggactgc aaagcgggaa tggcggaata
    24241 tcagcggcgt taatggcttc tgataattcg attgaccgtt ggcggatgcg gcgcgagtgc
    24301 cttgtccgcc cacaacacac cacaatcgta ggcctgagaa gacgcaacag cgtagcatcg
    24361 ggcaatcagc atcatacagt gccactcaaa acgtcaaact actttcacct aaaataccct
    24421 tatcacctga tatatagtaa acaccttacc tggcgcgaat ttctttgaat aacggccaac
    24481 cgccccgctt atttgcgcat cttttgtctc gatatacatt ttctgacatc ctcatccgct
    24541 atgctacagg cactaaaaat ggttattgag gagcaaatat ggctaactgg ttaaatcaac
    24601 tgcaatccct gcttgggcaa agcagttctt ctacctcctc gtctgcggat cagggattgg
    24661 gcaaactgtt agtgccaggc gcattaggcg gactggctgg gctgctggtc gcaaataaat
    24721 cagcacgtaa acttcttacg aaatatggca ccaacgcgtt actggttggc ggcggagcgg
    24781 tagcgggtac ggtgctgtgg aataaataca aagataaaat tcgcgcggcg catcaggacg
    24841 aaccgcagtt tggcgcgcaa agtacgccgc tcgatgagcg tacggaacgt ttgatccttg
    24901 cgctggtctt tgccgctaaa agtgatggtc atattgatgc caaagaacgt gcggcaatcg
    24961 accagcaatt gcgtgaagcc ggcgtggaag agcaggggcg ggtactcatt gagcaggcaa
    25021 tcgaacaacc gctggatcca caacgcctgg ctaccggggt ccgcaatgaa gaagaggcgc
    25081 tggaaatcta tttcctgagc tgcgcggcta ttgatattga ccattttatg gaacgcagtt
    25141 acctgaatgc actgggtgac gcgctgaaaa tcccccagga tgttcgtgaa ggcattgaac
    25201 gcgatctcga acagcaaaaa cgcactttgg cggaataatc ccgctctctg gatagcctga
    25261 agttgactat ttcgggctat ccagaaccgc acagccattt gaacaggctg attttctctg
    25321 tctgcataac attcgatatt cgcgccatta cctgtcatca tctaagcaat gactgccctg
    25381 tttcgcttgc atccccggtg agttttgcca cccttataag atgtttcaac cagaaagaac
    25441 aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca tggcgatacg
    25501 cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccgga agtcctggac
    25561 tacctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcacaaca agccttgcag
    25621 gatcgcatct taaaggaaat catcgaccgc attccgcaaa gagaagtttc tgcgccctac
    25681 atcaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata tgctatctac
    25741 cagcgtcaat cggcgttcag tgaagagtgg gacgagtggg aaatattgct cgatgccaac
    25801 aagcgcgcgg ctcatagtga gttttattcg atgggcggaa tggcgattac gcccgataac
    25861 accattatgg cgctggcaga agattttctt tcccgacgcc agtacggcat tcgttttcgt
    25921 aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc cagctttgtc
    25981 tgggcaaatg actcctggac tttctactat gttcgcaagc atccggtgac gctgctgcct
    26041 tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagataaact gatttacgaa
    26101 gaaaaagacg atacctatta cgtcagcctg cataaaacga cctcgaagca ctatgtagtc
    26161 attcatttgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga aatggccgat
    26221 gccgagccgt ttgtttttct gccgcgccgc aaagatcacg aatacagcct tgatcactac
    26281 cagcatcgtt tttatctgcg ttccaaccgc aacggcaaaa actttggctt ataccgtacc
    26341 cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgcgaaaa catcatgctg
    26401 gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg cgggttaacc
    26461 agtttgcgcc aaattaaccg caagacccgg gaagtcattg gtattgcctt tgatgatccg
    26521 gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt
    26581 tattcttcca tgaccacacc agacactttg tttgaactgg atatggatac cggtgagcgt
    26641 cgtgtattaa aacaaacgga agttcctggt tttgatgcgg cgaattaccg cagtgaacac
    26701 ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgttggtcta ccatcgcaaa
    26761 cattttcgca aagggcacaa cccgttgctg gtgtatggct atggttctta cggcgcaagt
    26821 attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggttt tgtctacgcc
    26881 attgtccatg ttcgcggcgg cggtgagctg gggcaacaat ggtacgaaga cggtaaattt
    26941 ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt gttaaaactg
    27001 ggctatggct ctccttcgct ttgttatgcg atgggcggga gtgcgggggg catgttgatg
    27061 ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca ggtaccgttt
    27121 gttgatgttg taacaacgat gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa
    27181 gagtggggga atccgcagga tccgcaatat tacgagtaca tgaaaagcta cagcccatat
    27241 gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt gcacgattct
    27301 caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat
    27361 gaccatcttt tattgctctg taccgacatg gactcaggcc atggcggtaa atctggtcgc
    27421 tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga
    27481 acattacccg ctacgcctgc ggattaagta ttttccagat aatgtttcag tgttaaacgc
    27541 agctccgggc tcatgctgtc caggttatta aataaccagc gcagatagcc cggatcgcgt
    27601 tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt cgtcatcaac
    27661 gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt gttcataata
    27721 tcgataagca acgcggcggt gatataacag tcatacaacg cgcggtgatg atgcaggccc
    27781 ggcggcgtct gtacattgag cttccgtgtt ttatataacg ccatattgct gtacttgatc
    27841 cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg catctcaggc
    27901 agtacgcggc ggtcaaagct ggcgttatgc gcgacatacc attcactacc gtaatagtgt
    27961 gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc
    28021 gccatcgctt gtggactaat aggacgatcg gggcgcacca ggtggctcat ggggttgacg
    28081 atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg caaaccgcag
    28141 gtttctgtat cgataatgcg caacatggca tgctccaggc cgaaaaagcc tagcgtaatg
    28201 gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat aatgaaccac
    28261 gatcggcgcg aacgatgaga cgaccatatt catcccatag tgcactgtta ccacgggcat
    28321 ttgccatcag cacggcaatc gagtactgat gtgaaaaaaa ttgtaagcga cgggccgacg
    28381 ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca
    28441 tatccatacc ttgcggttgt tcatcgacca ccgtgatggt tctggaacga cggcccagac
    28501 atgcaccatg actctgatgg tagatcccag gcgtttttcg ccacggtgcg aacaccgcaa
    28561 ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg gtcattcgcc
    28621 aggtcgttgc ggcataacaa agcgggtcta acagtgagag atcgggtggg gcaggaaggg
    28681 cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaac agctgacatt
    28741 gttgcctggc ggcggccctc acaaactcca gatgatgagc tacctgttcg gtgagtgagg
    28801 ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg acttcctttt
    28861 caaaatgact gcaaattatg ggtgcggttt agaaaatact cataatttag caggcgttat
    28921 gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag gtaaacgtga
    28981 cagattgacc gaagccaaac ggtgggcaat aagccgctcg cgaaaccagc tgcgcaaatg
    29041 ttcaggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc gttctttaaa
    29101 tgccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt gatccagttt
    29161 agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt tactaagagt
    29221 gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac aaggttatgc
    29281 tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc acgctccctt
    29341 cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg ggcacatgcg
    29401 catttaacgc atcagtatcc tgcggcaaac gcgcaagtga cagctgcccc gcaggcaatc
    29461 actttaaact tctcggaagg tgttgaaaca ggattcagtg gtgcaaaaat cacggggcca
    29521 aaaaacgaaa atattaaaac attaccggcg aagcgaaatg agcaggacca aaagcaactg
    29581 attgttccac tggccgattc gctgaaaccc ggaacctaca ccgttgactg gcgtgttgtt
    29641 tcggtggacg ggcataaaac gaaaggacac tacaccttta gcgtgaaata aatcatgctg
    29701 gcgtttacct ggatcgcgct gcgatttatc catttcacct cgctgatgct ggtttttggc
    29761 ttcgcaatgt acggtgcctg gctggccccc ttaacgattc gtcgcttgct gacgaagcgt
    29821 tttctgcgtt tacaacagca cgctgccgtc tggagtttga tcagtgccac tgcaatgctc
    29881 gccgttcagg gcgggttgat gggaacggga tggacagatg tattttcgcc aaacatttgg
    29941 caggctgttt tgcaaacgca gtttggtggg gtctggttat ggcaaattgt tctcgccctc
    30001 gtaacgttga tcgtcgccct tatgcaaccg cggaatatgc cacgtttgct gtttatgctc
    30061 actaccgcgc aatttattct gctggcggga gtggggcatg cgacgctgaa tgaaggggta
    30121 acggcgaaaa tccatcagac taatcacgcg attcacctga tttgtgcggc ggcgtggttt
    30181 gggggattac tgccggtgct ctggtgtatg caactcatca aaggccgttg gcgacaccag
    30241 gctattcagg cgctgatgcg tttttcctgg tgcgggcatt ttgcggtgat cggcgtactg
    30301 gcaagcggcg tgcttaatgc attgttgatt accggatttc cccccacgct tacgacttac
    30361 tggggccaac tcttattgct caaagccatc ctggtgatga ttatggtggt cattgcgctg
    30421 gctaatcgtt atgttcttgt accgcgcatg aggcaggatg aagatcgtgc ggcaccctgg
    30481 tttgtgtgga tgactaaact cgaatgggcg ataggtgcgg tagtgctggt gatcatcagc
    30541 ctgcttgcga ctctcgaacc tttttgatgg attacgaaaa tgatgaaaaa aagtattctg
    30601 gcgtttctgt tactcaccag ttctgcagcg gcgctggctg caccgcaggt gattaccgtc
    30661 agccgttttg aagtgggtaa agacaaatgg gcgtttaatc gcgaagaggt gatgctgact
    30721 tgcagaccgg gtaatgcttt gtatgtcatt aacccaagta ccctcgtgca gtatccttta
    30781 aacgatatcg cacagaagga agttgccagt gggaagacta aagcccaacc catttcggtg
    30841 attcagattg atgatcccaa caatcccggc gaaaaaatga gtctggcacc gtttatagaa
    30901 cgggctgaaa aactctgtta attacctaaa atagcctttt gatttccaat aaaaaaaccg
    30961 cctcagttct ttcaccagaa cgggcggttt ttaacattta agctgatgac caccgcgctt
    31021 tttattgacc attttgcacg caaactggaa aacctggcgt cgtcatctat tcttaaaggg
    31081 caaggcaact aagcctgcat taatgccaac ttttagcgca cggctctctc ccaagagcca
    31141 tttccctgga ccgaatacag gaatcgtgtt cggtctcttt ttatctgtta aaagccagaa
    31201 gcatttcctt cgctgacttt atagtcaacc ataacacaca ctctactgtc tgagtccagc
    31261 gttttttaac attcttgtta agattatgtg atctttagcg cgggaggaaa atattgatga
    31321 aacagcctgc gcccgtttat cagagaattg cgggtcatca atggcgacat atctggcttt
    31381 ctggcgatat acacggttgt cttgagcagt tgcgccgcaa attatggcat tgtcgttttg
    31441 atccgtggcg agatttactt atctcagtgg gagacgttat cgatcgtggg ccgcaaagtt
    31501 tacgttgtct gcagttactg gaacaacatt gggttcgtgc ggtaagaggc aatcatgaac
    31561 agatggcgat ggatgcgctg gcatcccagc agatgtcttt gtggttgatg aatggcggcg
    31621 actggtttat tgcgctggca gataatcaac agaaacaagc gaaaacggcg ctggaaaaat
    31681 gtcagcattt gccctttatt cttgaagtac acagccgcac cggcaaacat gttattgctc
    31741 atgccgatta tccagatgat gtttatgaat ggcaaaagga cgttgatttg catcaggtct
    31801 tgtggagccg ctcgcgatta agtgaacgcc aaaaagggca gggaattaca ggtgctgatc
    31861 atttctggtt tggtcataca ccgttgcgcc atcgcgtgga tattggcaac ctgcattata
    31921 ttgataccgg cgctgtcttt gggggcgaac tgactttcgt gcaattgcaa taattaaaaa
    31981 tcaccatact cctgtgctgg tcgccagaaa ccatctataa aatcctcaat cggaaaacaa
    32041 ccgccatggc ggatccgttg atcgctcata gaataaagac actgctgttc cgtgttgtag
    32101 acatccacaa caatatcttc acaaccgcca tccaggtagc aaacaaaaag taccagcgcg
    32161 aacatttcat ccccgaagtg tggtgccgta ccgttaagtt taggagagat tttacaacgg
    32221 gggaataacc aggacaaata acccgccatg ccgacgggtt ctttttggat caggcaagac
    32281 gcataatcca ggcatcagat tgttgatcgt aacgggtacg gtaaaggaca gagtgttcgc
    32341 catttagcag ggcagggata ctggtttcag catcccaggc atccagttgc atacataaat
    32401 cggggtcaga tttacaagga atggttaacg ttcgatcacc cggcgattcg ccgtgtaatt
    32461 cgccgtcgtc gatttcaaaa gcgcctatgc gcacactcgt tttcatcttc gtgctctcca
    32521 tttagctgcc gattgtggta cgaccagtcc gatcgcacca tttttcagcc tagttgaggc
    32581 cgccgaatgt gccagtcaaa aagtgcgctt tttttgtgcg ctgtcatgcg ttactggtaa
    32641 aaagtttccc gtcccgcacc agttcacgcg ggtagctgtt tttcagtcgc gaaccaatcc
    32701 gtttggctaa accaatcggc tgatgctgga aagtaaccaa cacgtcatcc gccactggcg
    32761 cggcttgcgg gtaaacatcg cgcccgcgat accactcctc cgcttcttgc ggtgtcagtt
    32821 caaaagcgtt ctcgttgtcg ggggaggcaa gggcaataac cgcttcatgc tgccagcgat
    32881 aacctttgtt atgcgtctcg gcaagtttaa tccccaagcg agaaaatcgg actttaccga
    32941 tcagggcttc aatgcccacc gggaacaacc acagttcttt gtcacgctgc caggggcgca
    33001 ggtttccatc ccagtttaag ccaacacttg cagccgcctg acgaatttgt gcggcttcgc
    33061 gatctttcac cgggctgaac gggaaattgc ccactttgta ttttggggtg ggtaaggcgg
    33121 gaatggcctg agttttacgc agacgagcaa cgaagaagcc ttcgcagtcg taaatttgtg
    33181 ggaaaacatg caaaaagcct tcttcggtca gcgctttgtt tgcaccaggg aagagatcgc
    33241 caagtggtaa aaactctact gcgtcggggt aagtctcttt aagccacagg caaacggatt
    33301 cgttttcttc ccggtttaag gtacaggtcg agtaaaccag tgtaccgcca gggcgtaatg
    33361 catgaaaggc actgtcgatc agttcccgtt gggtagctgc gatttcctga ttgctttctg
    33421 gtgaccagtt ttttagcgca tcgggatctt tacgcaccac gccttcgccg gagcagggag
    33481 cgtccagcaa aatggcatcg aacatttctg gcaatgccgc accaaacacg cggccatcaa
    33541 aatgtgtgag cgcaacatta ctgataccac agcggctgat attggcatgt aacactttta
    33601 cccgactggc ggaaaactca ttggcaagga ttgccccttc gttattcatc cgcgcggcaa
    33661 tttgcgtcgt tttggagcct ggcgcagctg cgacatccat cacccgctgt ggtgcattat
    33721 cgtcagcaaa caaggcggca acgggcaaca ttgaactggc ttcctgaata taaaacaggc
    33781 cgcttaaatg ctcggcggta ctacccaatg gcaatgcatc ttcattgtcg cgttcaatcc
    33841 agaaaccttc ttcacaccac ggaattggcg taagcgtcca gccataagga gcggttaatt
    33901 gcaggaaatc agcaacggag attttcagcg tattaacgcg aatgctgcgg cgcaacgggc
    33961 gctgacaggc ggcaagaaaa tcatcaaaag agagcgtcga aggcatcgct tcgcgcattt
    34021 gcgtcagaaa ggcgtccggg aaataaacgg tgtgttgggc cacgagcata taccactggc
    34081 aaaaaaacag gcgcgcagtt tagcataaac gctccggcgc aggcacgccg gagcagtggg
    34141 cattatttgg gaagcgcagt tccccattca cgccactctt tcggttcact ttcctgcaac
    34201 aggaagtgct tgccttcctg ggctttcggt gccagtggcg tacccggagg cgtggcgaag
    34261 gcgatgccgc cacggataaa ctggttaaag gtgccggttt tcactacgcc gcccgtcaga
    34321 ccaaaatcca gactgtaacc cgatgccaac cagaagacgg aattgttacg caccaggtgt
    34381 tgatagcgtt tactgatgcg catcgcaatc atcacgcgat ctgacaatgt ccccagcgtc
    34441 attccggtaa ccgtacctac ttccagacca cggaacagca caggcgtacc gatgcctaac
    34501 gaaccggctt ccggcgcttc aacaataatg cttaaaccat ccaggtaacg cgaatcagta
    34561 atggtggcct cttgtaattc aaagtcgcga cgaggattgc cacggcctgg ttcgacgttg
    34621 atatacggct ggaggatagt atctagatgc tcaacgccag ctgccgaaat ttgcggtgtg
    34681 accactgaga agcgcgtacc tccgcgagca aaagtctgga catattccgg atagagcacc
    34741 gcctttgcct gtacttcatt acgcgcggta atcagatcca gtgtctggat ttgcccgata
    34801 tcaataccga gatagcgaat tggcatcccg accgccagtt ttccggcatc gaaagcgtga
    34861 agcgtaatct gcccgccaac cgcacgggcc gctgtttcgg aagcatacag aatacgctta
    34921 tcgcctttac gctgactggc gctggcaccg ctgaggttat cgaagctaat ggctcccttt
    34981 aatgctctgg agagcgggga tgcctgtacg gtcaggccac taccattcag ctgaactttc
    35041 gccccgcctt ctgcccagaa cacgctattg ctggtcagaa ggttgcgata ctccggctta
    35101 atatgcagat cgatatcaaa cgcgttagct cgcggacgca cggtaataac ttcaccaact
    35161 tcaaatttac ggtacagcac taccgatcct gcctgcacat ccggcagcgt ctctgcactc
    35221 aaactcacgg ttgtggtggg taaatcgcta aggctgttct ccagcgcttt ttccagattg
    35281 gcatacagtg gatagctggc tttcatctcg cctttatcgc ccggcagaat acgtatcccg
    35341 ccgttaatcc attctgaggc gctggcaccg agaaactcaa cgccatccag ccccaccttc
    35401 acgtcgacac ggctgttgac gacaaatttg ctatcgcctt ttaccagttc tcgatgctga
    35461 ggctcgatgg cgacggtaaa ggtgacgcct ttgctggtga gtttacgatc gataacctgg
    35521 cctacctgca cgccgtgaag aatgagcggc tgacccgcat caataccgta actttccggc
    35581 gcggtcaggg tcagcgtcag aacatcaggt tcatgcagca gtgctttttc gcctggcaca
    35641 acaacgaact ctttgcgtgg ctcgccatcg ccgggtacca actcgaaggt tttgccagtc
    35701 agcagggcgc tgagattggc atcgctaagg gataatttcg gattacgtaa ttcgatgcgg
    35761 gtattttcac gaagcagggt aacgacgctg ggatcaacgg tcatttcccc agtgacttta
    35821 ccaccaggat ttaaatccag tttagttagc tgtccgactt ccagtccctg atacattaat
    35881 ggcgtcgagt cggcggttaa tccggcccca ctcggcagtt ccagttttat tattacgcca
    35941 cgctggctgt gggccagatc ttcatacaga ccaaaggtat cttccgcctc ggcaggtttc
    36001 gactcttcag gtgaatcgaa ggcaatcgca ccgttaacca gtgccgccag actttccagt
    36061 ttcaccttcg cgccactgat actgacgttg gcatcaacgc cagaaacatt ccagaaacgg
    36121 ctaccttttt tcaccaggtc agtaaaacgc cgctcgatca ggacatcaat caccacgcct
    36181 tgtttattgg gattgatggc atagtcgtag acttttccca ccgggatctt gcggaaatag
    36241 accaatgaac cgctgttcag cgaaccgaga tcgggggctt gcaggtggat catcagatcg
    36301 ccattgtcca gccgatattt cggttgggta tcgagcgcga caaagtgatc ctgctcttta
    36361 cctttacccg gcatcatgcc gatatagttc ccaccgacga gggcgtccag cccggagaca
    36421 cctgccaacg atgcttttgg cgtcaccagc cagaactgag tctcttcgcg cagtgcatct
    36481 ttcatatcgg acttgatgct gaccttgact tcaatcttac gaagatcgtc gctgaggctg
    36541 atatcctgca ctgttccgac ttcaacgccc tgataacgaa caggcgtacg gcctggaaca
    36601 ataccatccg ccgacataaa gtcgatggtg acggtattac cccggtcctg ataactgtcc
    36661 caaatcagcc aaccggcaat cattagcgcg atgaacggca gcagccagaa aggtgagata
    36721 cggcgtttat ttttaatctg cgcttcagtc gtcgaagcgg gcgtttcctg actcatgtgc
    36781 atcccaaagt aagcggctgt ccagccattc cacagcaaga atagtcaaaa ttaccgctgc
    36841 gccgaaataa aacgcagccg gtcccatagt aaaagcgagg atctgatcgc gattaatcag
    36901 cgacatggtt aaagatatga caaacaggtc gagcatcgac cagcgaccaa tccaggtcac
    36961 cattcgcagt aacagaatgc gtgtgcgtaa accttgctgg catttaaaat gaatgctcaa
    37021 cagtaaagtg aacatgacga tcactttagt aaacggtacc agaatactgg cgataaacac
    37081 gattcccgca acggcaatgt tgctacttgc cagcgacata atcccggaaa gaatcgtatc
    37141 ttcctgccgg ccaccattca gataaatgat agaaataggc aacaggttgg caggtaacaa
    37201 caaaacgatt gacgctaaca gcgccgccca gcatttttgc aaactatgac ggcgacgcag
    37261 gcgtagcgga atatgacaac gcgggcagcg accacgctga tcaggatagc cggtaaaatg
    37321 gcacccaaga cagacacgaa gtttctcgtc cctacgcgta gcggggcgct gcggataaaa
    37381 tcgctcccac agctcctcga cattaagatg tgacaacgtc accgtcgtta aaatcaccaa
    37441 cgccacaaaa gagaacaagc cgacacccgc ctggatatgg gcataatcct gtacctttat
    37501 agaagcaacg ccaatgccga ccaggtagat atcgagcatt acccactctt taagtcgctc
    37561 aagcatcagc agtaccggac gtaaattcat tcccagtcgg ttcccaaacc ataaatatgc
    37621 tatggaggac accagaatga ggggggcacc aataacgcag aaaaagacca tcgcccccgt
    37681 tatcgtatcg ccctgtttgg tcatttgcca gatgccttgc atgacattgg cgtcgatacg
    37741 aatacctaac agccagatat gcaacagcgg ttcgccccag gcaaacggca tcagcaacag
    37801 catggtgaag gccattgccg ccaggcgcgt tagcgaccag tcgcgcccgt cacgaatttt
    37861 tgcctgacag cgcggacaat aggcactttg atgagaattt atctcgggca ggctaaacag
    37921 catgtcacat tgcgggcaac gttggtaatc accacgcggc agttcctcgc cgattgccct
    37981 cactgttatc ttttttgtcg gcgtaatttg tggtgtgtta agagccatga cgtgccaaat
    38041 gtaataagaa ttaacattaa tcttaaccca tgatgggaat gatcttgagc attaacgcat
    38101 ttaatgctta ttttaatagg ctggcagttc caggtaagac aaccaagtga ttatataatg
    38161 aacaaaacag aattttacgc ggatttaaat cgcgacttta acgcgctgat ggcgggagaa
    38221 accagttttc tggcaacgct tgcgaacacc agtgcgttgt tatatgagcg tctcactgac
    38281 gtaaactggg caggttttta tttgcttgag gacgatacac tggtgctcgg accatttcag
    38341 ggcaaaattg cctgtgtccg gatacccgtc gggcgtggcg tgtgcggcac tgcggttgcc
    38401 cgcaatcaag tgcagcgtat cgaggatgtt catgcgtttg acgggcatat tgcctgtgat
    38461 gcggcgagta attctgaaat tgttctgccg ctggtggtga aaaatcagat tattggtgtt
    38521 ctcgacatcg atagtaccgt cttcggtcgc tttacagacg aggacgagca aggcttacgt
    38581 cagcttgtgg cacagcttga aaaggtgctt gcaacgacgg attacaaaaa attctttgcg
    38641 agcgtcgcag gataatcaac ggataacgta gcatttactg atagcgtcat tataatgacg
    38701 cctgttcatg cctgcgcttg ttggctacgt ccgttgtaat caggaaattt catggaaaat
    38761 caacctaagt tgaatagcag taaagaagta atcgcgtttc tggccgaacg ttttccccac
    38821 tgtttcagtg cggaaggtga agcgcgtccg ctgaaaatcg gtatttttca ggatttggtc
    38881 gatcgtgttg ccggggaaat gaacctgagc aaaacgcaat tacgatccgc tttacgtctc
    38941 tacacttcga gctggcgtta cctttacggt gttaaacccg gcgcaacgcg tgtcgatctt
    39001 gacggcaacc catgcggtga gctggacgag caacatgtag agcatgctcg caagcagctt
    39061 gaagaagcga aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa
    39121 gctgccgcag ctgctggtga gaaagaagac gcaccgcgtc gcgaacgcaa gccacgtccg
    39181 accacgccac gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag
    39241 aaagcgccaa aaacagcaaa agcacctcgc gaagaacagc acaccccagt ttctgacatt
    39301 tcagctctga ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc gatggatgcc
    39361 accgtattag aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg tatgtctttg
    39421 attgtgcgcg cagaacacct ggtgttctga aacggaggcc gggccaggca tgaacatgtt
    39481 ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacct tcgctgtaga
    39541 agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc atgcgacggt
    39601 gagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg acctcgatca
    39661 ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca gccacaacgt
    39721 gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag gcgatgaact
    39781 gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa agcgtcgttt
    39841 tgaacgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca ccggcaacga
    39901 cacttataac cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc
    39961 gctgtgggac agtaaagtca aattcgacga gttaagcctg aagctggcag gaaaaacgga
    40021 taaagaaatt cgtgaaaccc tgactcgacg ctacaaattt gccattcgtc gtctggcgca
    40081 aaccaacagc gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg aaatcgaccc
    40141 gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc
    40201 gctggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta tcaattcgat
    40261 ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca aaattgtcgg
    40321 tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg atgatgtggt
    40381 tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac ctgctggtaa
    40441 agggaccaag acccgcactg taacattgac ccgtgaacgt attcgtctcg aagaccgcgc
    40501 ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtactgg atattccggg
    40561 cttctatgtg ggtttgacag acgatgtcaa agtacaactg cagaaactgg aaaaacagaa
    40621 tgtcagcagt gtgatcatcg acctgcgtag caatggtggt ggggcgctga ccgaagcggt
    40681 atcgctctca ggtctgttta ttcctgcggg tcctattgtt caggtccgtg ataacaacgg
    40741 taaagttcgt gaagacagcg ataccgacgg acaggttttc tataaaggcc cgctggtggt
    40801 actggttgac cgcttcagtg cttcggcttc agaaatcttt gccgcggcaa tgcaggatta
    40861 cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg
    40921 ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc tgggttctgt
    40981 gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt
    41041 aacgccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga
    41101 agataacgcg ctgccgtggg atagcattga tgccgcgacc tatgtgaaat caggcgattt
    41161 aacggccttt gaaccggagc tgctgaagga acataatgcg cgtatcgcga aagatcctga
    41221 gttccagaac atcatgaagg atatcgcgcg cttcaacgcc atgaaggaca agcgcaatat
    41281 cgtttctctg aattacgctg tgcgtgagaa agagaataat gaagatgatg cgacgcgtct
    41341 ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga aactggatga
    41401 tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga atatcgcact
    41461 cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgcacccg tcaagtaata
    41521 tcaatcaggc acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag
    41581 atgctacaaa atgtaaagtt gtgtctttct ggtgacttac gcactatcca gacttgaaaa
    41641 tagtcgcgta acccatacga tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa
    41701 aagaaaatta tgatgcgaat cgcgctcttc ctgctaacga acctggccgt aatggtcgtt
    41761 ttcgggctgg tactgagcct gacagggata cagtcgagca gcgttcaggg gctgatgatc
    41821 atggccttgc tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg
    41881 gcattacgat ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg
    41941 ctggtcaata ctgtagcaac ccaggctcgt caggcgggga tcgctatgcc gcaagtggct
    42001 atctaccatg cgccggacat caacgctttt gcaaccggtg cgcgccgtga tgcctctctg
    42061 gttgctgtca gcaccggttt gctgcagaac atgagcccgg atgaagccga ggcggtaatt
    42121 gctcacgaaa tcagccacat cgccaatggt gatatggtca ccatgacgct gattcagggc
    42181 gtggtgaaca ccttcgttat ctttatttcc cgtatcctgg cgcagcttgc cgcgggtttt
    42241 atgggcggaa atcgtgatga aggtgaagag agcaacggca acccgctgat ctactttgcg
    42301 gttgcaacgg ttctggaact ggtgtttggt attctggcga gcattatcac catgtggttc
    42361 tcgcgtcatc gtgaattcca tgctgatgcc ggttcggcaa aactggttgg tcgcgagaaa
    42421 atgattgccg cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc aaccagcatg
    42481 atggctttct gcattaacgg taagtcgaaa tcgctcagtg agttgttcat gactcacccg
    42541 ccgctggata aacgaattga agctctgcgt acgggtgaat acctgaagta atattatgaa
    42601 gtaaccacta aaaagcgcgt cctgatcgac gcgctttttt ttatgccctg gatcgtggct
    42661 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
    42721 gcgagacgtg tgtaccatta tctccaaact gatttagcat cagcgccacc agcgccgcgc
    42781 cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca ccgctacgtt
    42841 cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag ccaaatccag
    42901 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
    42961 ccagggaaaa aagccccgca gccatgatga ataatcctaa agcacccagc aatcctgcat
    43021 gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct aacggccacg
    43081 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag gacggtttgc aggtaaaagg
    43141 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    43201 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    43261 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    43321 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    43381 cgtacatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggcg
    43441 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    43501 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    43561 agacggcaac aataaacgag tttatgccca tccctctacc cagaaaacgt tgtggataga
    43621 tcaggcggat aagggcggta ttaacgctca tcaacgctgc accgccgaaa ccttgtatga
    43681 cacgcgcaag ggtgagtatt tgcagcgaat cagaaagggc gcagaacagt gaagacaaca
    43741 gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatca cccagaaacg
    43801 aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc cagatggaac
    43861 tggctggcgt ggcatgaagg tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc
    43921 cgtcaaggtc agccatcgaa ataccaatca caatggttaa tatcgcaccg tatcgctggg
    43981 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttaaatctt tggtgaataa
    44041 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    44101 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    44161 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    44221 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    44281 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    44341 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    44401 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    44461 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    44521 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    44581 cgcatgtatt cacggattgg gcgtcgtaat ccactgtaca gcaccgcgat tggtaaggta
    44641 ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    44701 cgcagtaccg agcggaccat caccagtaca gaagcgttat taccggttct ggaccaggtg
    44761 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    44821 gttccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    44881 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    44941 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    45001 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtggcgt
    45061 tagattaaac gcagttgcta taagaatatt ttactcatga gtgagaatct ggttatttat
    45121 tacacttaac cattatcgac cacgatattg cttttgcgta acagcgggca atctgttatc
    45181 cccaaaaaac cacttttagt gtgcaagtat tgtaccgtgc tggtgcctct ggcagtcaga
    45241 taggtacatt gcaaacctaa tcctgcggca ttctctttgc ttccaatcaa aacgccatat
    45301 ccgctgagta ataatcctat ccataccagt gctatcagca taactgtgcg aatgatgaat
    45361 cgcattacaa cctcttctct ttttatgttc gcttaatcgt agcggcaata tgcgctgaag
    45421 caagcgactc attccgaaaa agcacgaata tcgacatagt taggcgctgt ttaactaacg
    45481 catgctagtt taatgacata aggtaggtga aacggagatt ggagtgaaaa agtttcgatg
    45541 ggtcgttctg gttgtcgtgg tgttggcttg cttgctgctt tgggcgcagg tattcaacat
    45601 gatgtgcgat caggatgtac aatttttcag cggaatttgt gccattaacc agtttatccc
    45661 gtggtgatat cattttttca gcactagatt ttcttcctct ccagcagtgg tagaatagcg
    45721 cctctactca gaggtggtga aatgaacgaa gttgtaaatt caggcgtgat gaacattgcg
    45781 tctttggttg tatcggtggt ggttcttctt atcgggctca tcttgtggtt ttttattaat
    45841 cgtgccagtt ctcggactaa cgaacagatt gaactgcttg aggcgttgct ggatcagcaa
    45901 aaacgtcaaa atgcactgtt acgtcgtttg tgcgaagcaa acgaaccaga gaaagcagat
    45961 aaaaagacca ttgagagtca aaaatcggtt gaagacgaag atattattcg cctggtcgcc
    46021 gaacgataat ccttcctcaa tcttgagtaa ccccgtcgtt gaagggttac tcaattcatc
    46081 ctgattatac gcactgtcat ggtgcaactg cttcaaatta aaactctggt ctgacttcaa
//
LOCUS       DAZGCQ010000029        45921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000029 DAZGCQ010000000
VERSION     DAZGCQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.029"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="ACHK07_001765"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="ACHK07_001765"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO8102108.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1543..2349
                     /gene="tcdA"
                     /locus_tag="ACHK07_001766"
     CDS             1543..2349
                     /gene="tcdA"
                     /locus_tag="ACHK07_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO8102109.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="ACHK07_001767"
     CDS             complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="ACHK07_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO8102110.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRKTFAPLTQWEEKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAEHGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="ACHK07_001768"
     CDS             complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="ACHK07_001768"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO8102111.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4240..4467
                     /gene="ygdI"
                     /locus_tag="ACHK07_001769"
     CDS             4240..4467
                     /gene="ygdI"
                     /locus_tag="ACHK07_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO8102112.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4818..5735
                     /gene="gcvA"
                     /locus_tag="ACHK07_001770"
     CDS             4818..5735
                     /gene="gcvA"
                     /locus_tag="ACHK07_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO8102113.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5754..6149
                     /gene="ygdD"
                     /locus_tag="ACHK07_001771"
     CDS             5754..6149
                     /gene="ygdD"
                     /locus_tag="ACHK07_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO8102114.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6142..7242
                     /gene="rlmM"
                     /locus_tag="ACHK07_001772"
     CDS             6142..7242
                     /gene="rlmM"
                     /locus_tag="ACHK07_001772"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO8102115.1"
                     /translation="MNKVVLLCRPGFEKECAAEISDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="ACHK07_001773"
     CDS             complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="ACHK07_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO8102116.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="ACHK07_001774"
     CDS             complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="ACHK07_001774"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO8102117.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8499..9917)
                     /gene="fucK"
                     /locus_tag="ACHK07_001775"
     CDS             complement(8499..9917)
                     /gene="fucK"
                     /locus_tag="ACHK07_001775"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO8102118.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10026..11801)
                     /gene="fucI"
                     /locus_tag="ACHK07_001776"
     CDS             complement(10026..11801)
                     /gene="fucI"
                     /locus_tag="ACHK07_001776"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO8102119.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11834..13150)
                     /gene="fucP"
                     /locus_tag="ACHK07_001777"
     CDS             complement(11834..13150)
                     /gene="fucP"
                     /locus_tag="ACHK07_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8102120.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13697..14344
                     /gene="fucA"
                     /locus_tag="ACHK07_001778"
     CDS             13697..14344
                     /gene="fucA"
                     /locus_tag="ACHK07_001778"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO8102121.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDTVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14372..15520
                     /gene="fucO"
                     /locus_tag="ACHK07_001779"
     CDS             14372..15520
                     /gene="fucO"
                     /locus_tag="ACHK07_001779"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO8102122.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIICNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15575..16330)
                     /gene="xni"
                     /locus_tag="ACHK07_001780"
     CDS             complement(15575..16330)
                     /gene="xni"
                     /locus_tag="ACHK07_001780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO8102123.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMLALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16442..17809)
                     /gene="sdaB"
                     /locus_tag="ACHK07_001781"
     CDS             complement(16442..17809)
                     /gene="sdaB"
                     /locus_tag="ACHK07_001781"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO8102124.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17867..19156)
                     /gene="sdaC"
                     /locus_tag="ACHK07_001782"
     CDS             complement(17867..19156)
                     /gene="sdaC"
                     /locus_tag="ACHK07_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO8102125.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19713..21077)
                     /gene="ppnN"
                     /locus_tag="ACHK07_001783"
     CDS             complement(19713..21077)
                     /gene="ppnN"
                     /locus_tag="ACHK07_001783"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO8102126.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKHTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21189..22037)
                     /gene="queF"
                     /locus_tag="ACHK07_001784"
     CDS             complement(21189..22037)
                     /gene="queF"
                     /locus_tag="ACHK07_001784"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO8102127.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGADIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGNVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22105..22650
                     /gene="syd"
                     /locus_tag="ACHK07_001785"
     CDS             22105..22650
                     /gene="syd"
                     /locus_tag="ACHK07_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO8102128.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23272..23601
                     /gene="yqcC"
                     /locus_tag="ACHK07_001786"
     CDS             23272..23601
                     /gene="yqcC"
                     /locus_tag="ACHK07_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO8102129.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDVPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23601..24383
                     /gene="truC"
                     /locus_tag="ACHK07_001787"
     CDS             23601..24383
                     /gene="truC"
                     /locus_tag="ACHK07_001787"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO8102130.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24401..24850
                     /gene="yqcA"
                     /locus_tag="ACHK07_001788"
     CDS             24401..24850
                     /gene="yqcA"
                     /locus_tag="ACHK07_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO8102131.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25285..26637
                     /gene="gudP"
                     /locus_tag="ACHK07_001789"
     CDS             25285..26637
                     /gene="gudP"
                     /locus_tag="ACHK07_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO8102132.1"
                     /translation="MSSLSQAASRVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26639..27979
                     /gene="gudX"
                     /locus_tag="ACHK07_001790"
     CDS             26639..27979
                     /gene="gudX"
                     /locus_tag="ACHK07_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO8102133.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28000..29340
                     /gene="gudD"
                     /locus_tag="ACHK07_001791"
     CDS             28000..29340
                     /gene="gudD"
                     /locus_tag="ACHK07_001791"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO8102134.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29573..32329)
                     /gene="barA"
                     /locus_tag="ACHK07_001792"
     CDS             complement(29573..32329)
                     /gene="barA"
                     /locus_tag="ACHK07_001792"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO8102135.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32386..33687
                     /gene="rlmD"
                     /locus_tag="ACHK07_001793"
     CDS             32386..33687
                     /gene="rlmD"
                     /locus_tag="ACHK07_001793"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO8102136.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33735..35969
                     /gene="relA"
                     /locus_tag="ACHK07_001794"
     CDS             33735..35969
                     /gene="relA"
                     /locus_tag="ACHK07_001794"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO8102137.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36047..36295
                     /gene="mazE"
                     /locus_tag="ACHK07_001795"
     CDS             36047..36295
                     /gene="mazE"
                     /locus_tag="ACHK07_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO8102138.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKEVW"
     gene            36295..36630
                     /gene="mazF"
                     /locus_tag="ACHK07_001796"
     CDS             36295..36630
                     /gene="mazF"
                     /locus_tag="ACHK07_001796"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO8102139.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36701..37492
                     /gene="mazG"
                     /locus_tag="ACHK07_001797"
     CDS             36701..37492
                     /gene="mazG"
                     /locus_tag="ACHK07_001797"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO8102140.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37720..39357
                     /gene="pyrG"
                     /locus_tag="ACHK07_001798"
     CDS             37720..39357
                     /gene="pyrG"
                     /locus_tag="ACHK07_001798"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO8102141.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39445..40743
                     /gene="eno"
                     /locus_tag="ACHK07_001799"
     CDS             39445..40743
                     /gene="eno"
                     /locus_tag="ACHK07_001799"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO8102142.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40803..41705)
                     /locus_tag="ACHK07_001800"
     CDS             complement(40803..41705)
                     /locus_tag="ACHK07_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO8102143.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLRTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(41702..42850)
                     /locus_tag="ACHK07_001801"
     CDS             complement(41702..42850)
                     /locus_tag="ACHK07_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO8102144.1"
                     /translation="MRIFLLLLMLCSFQVFAERLPSVNDLTGTLTTEEQSVLNQQLQT
                     LEQQNHLQVAVLVTPTTGGKNIKLAASQSYGLYHWKPIGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPKQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASIKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            complement(43087..43758)
                     /locus_tag="ACHK07_001802"
                     /pseudo
     CDS             complement(43087..43758)
                     /locus_tag="ACHK07_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(43772..44374)
                     /locus_tag="ACHK07_001803"
     CDS             complement(43772..44374)
                     /locus_tag="ACHK07_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO8102145.1"
                     /translation="MLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            44547..45218
                     /gene="queE"
                     /locus_tag="ACHK07_001804"
     CDS             44547..45218
                     /gene="queE"
                     /locus_tag="ACHK07_001804"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO8102146.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   45557..45890
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=45557..45585
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT        11448 a        11108 c        12060 g        11305 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttggc atgattttga acaaaaacaa atcactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggaa acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc gggggcattg agtgataaat atattctcgc ttacagtaac
      721 tccctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcactggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tcttttgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggtc gcgcttcagt tgcctctgat cgttccatta ttccgccagg tacaaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgggtttgg
     1261 gtgctgaaaa cggctccggg cgcaggtaac gtctttagtg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac atcctgatta ttgactaagt
     1381 gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa tcatgcaaat
     1441 ccaaaatatt gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt
     1501 ttagggtgag ggataacctc gcccttttta ttctgaggtg ctatgtctgt ggtaattagt
     1561 gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgctgcaa
     1621 ctgtttgctg acgcgcatat ttgtgtggtc ggtattggcg gtgtcggttc ctgggcggcg
     1681 gaagcgctgg cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc
     1741 gtcaccaata ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg
     1801 gaagttatgg cggagcgtat tcgccagatt aacccggagt gccgtgtaac ggtggtggat
     1861 gatttcgtga cgccggataa cgtagcgcag tatatgagcg tgggttattc gtacgtgatt
     1921 gatgccattg atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa
     1981 atcccactgg tcacaaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt
     2041 accgatctgg cgaaaacgat tcaggacccg ctggcggcga agttgcgtga gcgcctgaaa
     2101 agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg gcgtcgattg cgtgttctct
     2161 accgaagcat tggtgtaccc gcagtcagac ggtacggtgt gtgctatgaa agccacggca
     2221 gaagggccga agcggatgga ttgtgcatct ggatttggcg cggcgacgat ggtgaccgcc
     2281 acctttggtt ttgttgcggt ttctcatgcg ctgaaaaaga tgatggcgaa agcggctcgt
     2341 caggggtaaa taatcaggtc ggatgcgacg cgaacgtctt atccgacctg gtactgtgct
     2401 taaacctgct tcgccgcagc gataatcgcc tcgcttaacg cattcagccc ctggctgcgt
     2461 gaggcgctaa gttgcgcacg caatcccagc tcatcaaaca atgccagcgg cgactgtgcc
     2521 tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca gcaccgccag caggccgcgc
     2581 actatacgtc cttcgctgtc gccaaagaag tgcattttac cgtgttcagc cacggtatat
     2641 cccagccaga cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct
     2701 ggcaaagccg gaagctgttt ccccagcatg atcaactggc gatatttttc ttcccattgc
     2761 gtcaacggtg cgaaggtctt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt
     2821 ccggcgaatt gcgggttcgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat
     2881 tcaccagcgc atccacatca ctctttgtat tatatggcgc aaaagaggcg cgcagtgtgc
     2941 cggttacgcc taattctgcc agtagcggct gagcgcaatg ttgcccggcc cgcaaggcaa
     3001 taccgtactc cgccagcagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg
     3061 ccagcaggct ggaatcctga cagcggaatg aacggaagcc tggacgtttc gccagtgcat
     3121 cttccgccag tgttgctaaa ctacggctcc agctttcggc ctggttgata tcgtaatctg
     3181 ccagccattc cagcgccgcg cttaatccta tgacaccagc cacatttggc gttcctgctt
     3241 ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa actcacttcg tgaaccattt
     3301 tgccgccgcc tagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc
     3361 cgatacccgt cgggccatac agtttgtgac cagagaaagc atagaaatcg atatccagtt
     3421 gctgaacatc cgcggggaaa tgcaccgccc cctgagcacc atcaaccatc accaccatcc
     3481 cggctgaatg agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtaacgttcg
     3541 acatctgacc caacgccaga atccgactac ggggagtaat cagttctggc aacaaatcga
     3601 catccggcag tcgctgcgca ttaagcggca atttcaccac tttggctcca gtttgttggg
     3661 cgaccatcag ccaggggacg aggttagcgt ggtgttctgc cacgctgaca ataatctcat
     3721 cgcccggttg cagacgcgga cgcgcatagc attgtgccac catgttgatg gattcagtgg
     3781 tgccgcgcgt ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct
     3841 ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga
     3901 cgtttccggc gctcaggctg taaaactgtc gagtggcttc aaccacggct tcaggtttaa
     3961 gcgcggtcgc ggcgctgtcg agatagacgc ccgcatcctg tagtgcggga aactgggcgc
     4021 gaaactgcgc gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac
     4081 gcaaaattca ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc
     4141 gaagccgcgt cagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca
     4201 atactattaa gatttattaa tcgctttaag gagaagatga tgaaaaagac tgccgcaatt
     4261 atttctgcct gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg
     4321 cacaccaatg acggacgtac catcgtctct gacggcaaac cacagactga taacgatacc
     4381 ggtatgattt cgtataaaga cgctaatggc aacaaacagc agatcaaccg taccgacgtg
     4441 aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa
     4501 ttaggcggtg ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa
     4561 aggtagcttt gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat
     4621 cacaaccgta agccaaaagt tcaccagaac acgcattccg ataaaacttt tcgttccggc
     4681 tcaggaagtg ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg
     4741 gctcagatta aaaaaactaa taggttacac agtgtgatct aattgttaaa ttcatttaac
     4801 atcaaagttt aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg
     4861 atgccgcagc acgccattta agtttcactc gcgcagcaga agagcttttt gtgacccagg
     4921 ccgcagtaag tcatcaaatc aagtctcttg aggattttct ggggctaaaa ctgttccgcc
     4981 gccgtaatcg ttcactcctg ctgaccgagg aaggtcaaag ctatttcctc gatatcaaag
     5041 agatattttc gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg
     5101 cgttgacggt cagtttactc cccagtttcg ccatccattg gttggttccg cgactttcca
     5161 gctttaattc agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag
     5221 ataagctggc ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc
     5281 tacgggtgga aaaactgtac gccgaatatt tattgccggt gtgttcgccg ctactgctga
     5341 ctggcgaaaa acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg
     5401 cgtcacgccg tgactggcag acatatactc gtcaattggg gttaaatcat atcaacgttc
     5461 agcaagggcc aatttttagc catagcgcca tggtgctgca agcggctatt cacgggcagg
     5521 gagtggcgct ggcaaataac gtgatggcgc aatctgaaat tgaagccgga cgtcttgttt
     5581 gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta tctggtttgt catgacagcc
     5641 aggcagaact gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg
     5701 aacaagaaaa attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca
     5761 gccgttttat gctgattttc gccgccatta gcggcttcat ttttgtggct ctgggcgctt
     5821 ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccaaaccg
     5881 gcctcgaata ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc
     5941 gtcgcatcag tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt
     6001 tcagcggcag cctttattgc ctggcactgt cccatctgcg tttgtgggcg tttgtcactc
     6061 cggttggcgg cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt
     6121 taaagcgcaa gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa
     6181 aaagagtgcg ccgcagaaat tagcgataaa gccggccagc gggaaatttt cggttttgcc
     6241 cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat
     6301 aagttaatcc gtgagttgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg
     6361 ggggaactcc tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta
     6421 cagggcgtag tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc
     6481 aaagagttgc tgaaattctg ccgtaaattc accgtgccgc tacgcgctgc cttgcgcgat
     6541 gcgggggtgc tggcgaacta tgaaacgccg aagcgtccgg tagtgcatgt attcttcatt
     6601 gcaccaggct gctgttatac cggttactca tacagcaaca ataattcgcc gttctatatg
     6661 ggcattccgc gcctgaaatt tccggcagat gcgccgagtc gttccacgct caaactggaa
     6721 gaggcatttc atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg
     6781 tgggcggtgg atttgggcgc ttgcccaggc ggctggacct accaactggt gaagcgcaac
     6841 atgtgggttt attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag
     6901 gtgacgtggc tacgggaaga cgggttcaaa ttccgtccga cacgcagcaa tatctcctgg
     6961 atggtatgcg atatggttga aaaaccggcg aaagttgcgg cattgatggc gcagtggctg
     7021 gttaatggct ggtgccgtga aaccattttc aacctcaaac tgccgatgaa aaaacgctac
     7081 gaagaagtgt cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat
     7141 gctcagattc aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc
     7201 cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt
     7261 cgtctccggc ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct
     7321 catcagaaat aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat
     7381 taaatttact tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt
     7441 tgtaatcagc gttgatggca ttggagtccc acagtgcgcc gctgctatcg atcccttcac
     7501 aagaaaaaat aaacagatcg atttccagcg atttaagttg ggaaatcagc gagggattga
     7561 cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc
     7621 gtttaccgag ttcatggcaa atcggatggc tattggtgaa gacctgaatg ttgatgtcag
     7681 gcaactggcg tgccagatac cagcaggttg aactggcgtc taaggctatc accatccctt
     7741 cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca
     7801 gcctgatgtg aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgtc
     7861 catgattgcg cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt
     7921 ctttactgac ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca
     7981 gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata
     8041 accgcccgcg tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa
     8101 gaatattccc gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc
     8161 gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa
     8221 gcgcattacg gtaacgtcgt tctacttcag gatcgagagt gtcaccttct accgctgcca
     8281 tcatcaccag cggcggtgca taactgtcca gttcaaataa cgggataatc gcctggagca
     8341 agtcgctcac caacaggcca tcagcgcgga tcacctgcgg tcccatcgaa tgggcgggaa
     8401 agtgagcatc ggaaaaaata atttcatctc catgtcccat ctctgccagc actttcaata
     8461 gttcgggaga aattaacggc gaaattgttt tcagcatttc acacttcctc tataaattca
     8521 ggttcagttt gcgggtagaa ataacggtac tgataatgaa tctgcgcgcg ggcttcttcc
     8581 gggctgttaa attcccctat gccataccaa ccgaacagcg cagctcctgc gacggtcgtt
     8641 tcggcgtcgt cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac
     8701 aatgtgttgc gacttcctcc accgactaac aataattcag aggctttaaa gtgcccgatt
     8761 ttttccagca tctgtagatt gcgctgtaat tgcgcagtta acccttccag cgccgcgcga
     8821 tagaaatgcc cccgcgtggt attaagcgtc actccttgcc agccagcgtt ctgacacgac
     8881 aataaatcac actgcatttt cacgccatcc gcgccaggcg cgatcagacg agcctcttca
     8941 atcaacattt gccagggtgt ttcagccgtc cagaacagtt ttctcaccca ttccagcacg
     9001 ccggatgcca gccattgcat acctgggtta tacaaccctg cctggctatc cagttcgcag
     9061 gtggaaccgg cgtactgact taacagcgaa gtatcaacct gggcgctgcg aaccattaaa
     9121 atttcccatg taccggaaga gagcacgggt tcattttgtt cagcaccagc gccaaaaagg
     9181 gcgaactggg tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt
     9241 gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag
     9301 agtcggcgtg gaataccggt ggcttgtaaa atttgtggac tgaaatcgcg ttgctggata
     9361 tccagcatct ggctggttcc ggccatcgtg atatcagtag tgaattcgcc ggttaaacgg
     9421 tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc gttccagcag ttgtggatga
     9481 ttttctttca accacaccaa cttatataac gtattgaaac taaaggctcc gacgccagaa
     9541 atagcctgca accgctgtgc ggagattaac cgttcaatat tgtccataac cgctgctgtt
     9601 cgcggacatt tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg
     9661 ccatccacac caaaggtggt gacggcgata ccgcggatgt ggcattcagt cagttcacta
     9721 ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg cgtctaaaga ccactggtgc
     9781 caggtgttgt tttccatcgc gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt
     9841 tttccctgcc gattaaccgc gatggccctg acattggtcg cgccacagtc gagtaccagg
     9901 ataacttctt gtttcataat cgctcctgca atatagccgg ataacattgc ttatccggct
     9961 aaccactctt gcttgttcag gcccggtagc cggagcgacc gggcatcaca tcagggagta
    10021 atgtattaac gcttgtacaa cggaccgtag ttctggcaag cgcggtaatc ctggccttca
    10081 atatccatgc cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg
    10141 tgcatacata ccgggatacg cagcatggag gcgagagtga taaagtctgc gccaacgtgg
    10201 ccgatggtca gaaccccatg gttagcgccc cagttcgcca ttaccgagta cacatccgta
    10261 aacggccctt taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg
    10321 cgtttgttga ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt
    10381 tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg
    10441 ccttcggtaa ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg
    10501 caccattcgg tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc
    10561 ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa
    10621 cctgagttga tcaaatggat gatgccgtgt tctgccagtc catcgagttt atgccccgtt
    10681 acacgctcaa ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg
    10741 ccggtgagct ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc
    10801 acgacaaagg gttcgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcggcg
    10861 gtgtcaccat tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg
    10921 gcgttgtagc caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc
    10981 atcatgtcgc ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc
    11041 tcggcattac gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca
    11101 gcccaggcca gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga
    11161 cgcagttcgg tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg
    11221 tgatcaacaa tagaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta
    11281 cctttcatgc tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca
    11341 tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc
    11401 gggatgcctt tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt
    11461 tcagtgccgt taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca
    11521 ctgccatagc accagcaagg cgttaccgta atggtgaggc ctacattctg actgctgaat
    11581 ttttcttcgc aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac
    11641 tcgacggcag ctccgcaggc atggcgcagt ttctcggtca gcagtgcggc cgtagctttc
    11701 gccatattca ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata
    11761 accgggcgga taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat
    11821 tcggaaaata tgttcagtta gttgccgttt gagaacggaa acgggcaaag ataaagatga
    11881 ccgcgaagca gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga
    11941 caaaacccat gaccggagtg acaataccgc cgccaatgat ggtcataacg atgaaggacg
    12001 aaccgtattt ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact
    12061 gaatcgacat aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga
    12121 aggctgagat caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg
    12181 gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg
    12241 ttccggttaa atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt
    12301 agcgaatcaa atagctccag caggccgttt gtgcgccaac gtagcagaat tgcgctaata
    12361 ctgcccagcg ccagtggcga atacgcgcca ggcgagaaag cgatgcggag aacgatcctt
    12421 gtttggcgtc actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca
    12481 gggcgaccag taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca
    12541 ggctgtgttt atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt
    12601 gatgtggcac gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa
    12661 acgagttaaa tgtttgcgca agatttaagc ggaagtgacc actactttcc ggccctaata
    12721 ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa tccggctgca ataataaata
    12781 ggccaactaa aaataaggtg tagttcatta tttctgccgc gggccagaat aatgcagcac
    12841 ccaaggcata taaaaataac ccggtaataa tccctgcttt ataactgagt tttttcatca
    12901 atatcccagc aggaattggg ataatgaaat aaccaaagta aaaggccgat tggatcaggc
    12961 ctgcctggaa atttgtcagc gtaaaagcct gctggaattg aggtaataaa atgtcgttaa
    13021 ggttattggc taccgcccaa agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt
    13081 aacttctgct ttgccctgca tctttatcta ccgcacggta actctgcgtt tgtattgatg
    13141 tgtttcccat agcatcctct taggttcaga agcttaagca cgtaatcggt tatgttttca
    13201 aaagtgcaaa accagcgagt tcgggcttca tatcagtgaa tatttgcctg tctgaaatga
    13261 actcatggat tcattttcaa ataaaaaacg ctctgtaata tgacggcggt cacacttatt
    13321 agctggaagg tgactttatg tgactaccat cactttaata atcgaaattt catgcccgtt
    13381 tcagaaaata aatcataaaa aacacaaacc agatgaccgt attaattgtt tatgccgaaa
    13441 acgggcattc ctggcttagg tgaatagaaa aatgaccgtt ttgataaata tttttaagag
    13501 cggtcatttt taatggggtt ataaaaagag aacttgcagt tatttactgt gattagttga
    13561 gccaggtcac aaaatataag cttgtgaatt tctttcttgt taatcaggaa gaagatggtg
    13621 ggtaattaaa cggctaattc aatagtgtga aaggaacaac attattgccc tgttttgaat
    13681 cagagagagg tagctaatgg aacgaaataa acttgctcgt cagattattg acacttgcct
    13741 ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg aacgtcagtg tacgttatca
    13801 ggatgggatg ctgattacgc ctacaggcat tccatatgaa aaactgacgg agtcgcatat
    13861 tgtttttatt gatggcaacg gtaaacatga ggaaggaaag ctcccctcaa gcgagtggcg
    13921 tttccatatg gcggcctatc aaagcagacc ggatgccaac gcggttgttc acaatcatgc
    13981 cgttcattgc acagcagttt ccattcttaa ccgaccgatc cccgctattc actacatgat
    14041 tgcggcggct ggcggtaatt ctattccttg cgcgccttat gcgacctttg gaacacgcga
    14101 actttctgaa catgttgcgc tggctctcaa aaatcgtaag gcaactttgt tacaacatca
    14161 tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta tggctggcgc atgaagttga
    14221 agtgctggcg caactttacc tgacgaccct ggcgattacg gacacggtgc cagtgctgag
    14281 cgatgaagag attgccgtag tgctggagaa attcaaaacc tatgggttac ggattgaaga
    14341 gtaatttcgt aaagcaacaa ggagaaggat gatggctaac agaatgattc tgaacgaaac
    14401 ggcatggttt ggtcggggtg ctgttggggc tttaaccgat gaggtgaaac gccgtggtta
    14461 tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa tgcggcgtgg tggcgaaagt
    14521 gaccgataag atggatgctg cagggctggc atgggcgatt tacgacggcg tagtgcccaa
    14581 cccaacaatt actgtcgtca aagaagggct cggtgtattc cagaatagcg gcgcggatta
    14641 cctgatcgct attggtggtg gttctccaca ggatacttgt aaagcgattg gcattatctg
    14701 caacaacccg gagtttgccg atgtgcgtag cctggaagga ctttccccga ccaataaacc
    14761 cagtgtaccg attctggcaa tccccaccac agcaggcact gcggcagaag tgaccattaa
    14821 ctacgtgatc actgacgaag aaaaacggcg caagtttgtt tgcgttgatc cgcatgatat
    14881 cccgcaggtg gcgtttattg acgctgacat gatggatggt atgcctccag cgctgaaagc
    14941 tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg tatattaccc gtggcgcgtg
    15001 ggcgctaacc gatgcactgc acattaaagc gattgaaatc attgctgggg cgctgcgagg
    15061 atcggttgct ggtgataagg atgccggaga agaaatggcg ctcgggcagt atgttgcggg
    15121 tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt atggcgcatc cactgggcgc
    15181 gttttacaac actccacacg gtgttgcgaa cgccatcctg ttaccgcatg tcatgcgcta
    15241 taacgctgac tttaccggtg agaagtaccg cgatatcgcg cgcgttatgg gcgtgaaagt
    15301 ggaaggtatg agtctggaag aggcgcgtaa tgccgctgtt gaagcggtgt ttgctctcaa
    15361 ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt gtacgcaagg aagacattcc
    15421 ggcactggct caggcggcac tggatgatgt ttgtaccggt ggcaacccgc gtgaagcaac
    15481 gcttgaggat attgtagagc tttaccatac cgcctggtaa atgcgctgat gtgataatgc
    15541 cggatacgac gtttgcggcg tatccggctc gccgttaccg taccaatcgc aattgctgta
    15601 aattgccatc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac agaaacgcca
    15661 tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc agattctcat
    15721 atatcccttc cagactctga aactcgacca gcagctgtgt ggcgcttttt ggtccgattc
    15781 ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa tcgggcaact
    15841 gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa cgtttctgga
    15901 agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta tctgtcgaaa
    15961 caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg gctaagtcat
    16021 cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag gcggcgcgta
    16081 atgcaagcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct ttgtaatctg
    16141 gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg gcgacagcgt
    16201 gggttggctg gctgtgcata atgagctgat cgagcgcgtg ctggcaggtt tcgacacagg
    16261 gcgaaccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca ataagcaaat
    16321 gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg atactggaac
    16381 aggctatggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct ctggagagcg
    16441 attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg cggtacttgg
    16501 cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg cagacgcgcg
    16561 gctcgctggt acggcgcagc gccatacgtg cggcgttcac cgcttttact gccgcaatgg
    16621 cgttacgctc gatgcatggc acctgtacct gtccggcgac cgggtcacac gtcagaccga
    16681 ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt gcgcctaata
    16741 gttctgccag accagccgcc gccattgagc acgccacgcc aacttcaccc tggcaaccca
    16801 cttcagcacc agaaatcgac gcgttcatct tataaagaga accaatggcg ctggctacca
    16861 gcaggtaacg agccagtgag ttagcgttca cttcgcggat aaacttatcg tagtacgcca
    16921 gcacagccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg cgaccgccag
    16981 cggcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca accgccatcg
    17041 ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca gcacggcgtg
    17101 gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca ataccgccgc
    17161 gcatgacttc ccagacgttc gccaggtgct gttccagctc ttctttgctg tgcagcgcca
    17221 gctcgttttt catcatcagg ccagagagtg acagcccggt ttcctgacaa tgtttttgca
    17281 gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctatcc tgctggccaa
    17341 aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctga ctgtaaacga
    17401 ctttatcacc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa aggttgtcgg
    17461 catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg tttgccagca
    17521 tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg atatccacgg
    17581 tatccggcag gttacccgcc agacccataa ttatggcgat atcagtgtgg tggcctttac
    17641 cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct ttaagcaggt
    17701 tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca acggtatgag
    17761 aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata ggaaatacat
    17821 cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag ctgaacagag
    17881 agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg ttgctgatgt
    17941 gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc atcaggaaca
    18001 ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg ctcgggttca
    18061 gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg ttcagcttgt
    18121 tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg ttgaagcctt
    18181 cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc gcgataatcg
    18241 gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac agaatcgaga
    18301 tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtaca cagctgaaga
    18361 cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc ttcgagcatt
    18421 tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa gagatgatcg
    18481 gagagtggtt gaacgagaac accattaccg gaattgccag ccacagggtc atccacagac
    18541 cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg ccgttccact
    18601 gcgggatcag gtacagagcc agcagcatca atacgccaac aaacgggaac accagaatac
    18661 tcatcgcttt aacgatcatc tgctcaccga agcgaacgat ggtcatcata cccacgatca
    18721 ggatcagcga cagaatcgca cgcggcggtg gcgtcatacc cagctggtga gacatgaagc
    18781 tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtaa atagcgaaga
    18841 agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct acaacctcgg
    18901 tgatgtcttc gcccgggttt ttaccagaca gtacgaagcg agtcaggccg cggtgagcaa
    18961 aaaacgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata ccgccaacac
    19021 cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa aggcccagca
    19081 tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca atcgtgctgg
    19141 tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct tttagccagt
    19201 tcagcccggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga tggcttaatc
    19261 atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta tctatcagtg
    19321 atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg ctttactctg
    19381 taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga catcttactt
    19441 cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt tagttaatca
    19501 tggcgataat ttctttttca tagcgataaa atacggttta tgaaaatagg gaaacgtttg
    19561 cgttgcaata taaattgtat gacatattaa ctgtgatgat atttgtcgcg tgagggtggt
    19621 aagaaacatt catataacaa atgaaatgag ttgatgagta agtgtccaaa aacgagaaaa
    19681 cccggcaaca aacgtcaccg ggggagggga gattacgtgc agatttcgta gcaagggatg
    19741 taggctgagc ctggcaactt catacgatgc tgggcaacaa aaccctgtag caggtcgtcc
    19801 atacgacgca taatctcttt atcgccgttg attttgtaag gaccaaactc ttcaatggcg
    19861 cgaataccga cttcttttac gttacccgcc acaataccgg agaacgcacg gcgcaggtcg
    19921 gcagccagca cttcaacagg ttgatccggg taaagcttca gattagccat attctcgtga
    19981 gacggctcaa acggcatttg caaatctggc gcaatgcgca ttgaccagtt aaaactgtaa
    20041 gcgtcaccgg tgtcgcggcg attttctttc accagcggca tcgatttttt catctgacga
    20101 gcgacttcag cggcgtcatc aatgatgatg cggtaatggc ggcgcgcgtt ttcgcccagc
    20161 gtatgtacga caaactcgtc cagtacgcgg aagtagtcgg cgctctcttt cgggccggtg
    20221 aggatcaatg gtaaaacctg atctttgttg gccgggttca ttaaaattcc cagcaaatac
    20281 agcaactctt ctgccgtacc cacaccgcca gggaagataa tgataccgtg agcgatacgg
    20341 acaaacgctt ccagacgttt ttcgatatcc ggcatgatga tcaattcgtt gaccagcggg
    20401 ttaggcggtt cagcggcgat aatcgacggc tctgtcatac caataaaacg actgtctttg
    20461 taacgctgct gcgcgtgtcc gaccgcagca cctttcatcg gcgcttccat cgctcccgga
    20521 ccacagccgg tgcagatatt cagctcacgc aggcccagct ggtttccgac gcgacgggca
    20581 tacaaatact cattttcgtt aattgagtga ccgccccagc agaccaccat atttggcgct
    20641 tcacccacat gcagcgcgcg agcgttacgc aagatggaaa agaccaggtt agtgatgtgg
    20701 acggagttgt cgagattcag gttgggaaaa cgaacggtgt tatggatttg cccgtaaacg
    20761 aagagaatgt ctcgcagaac cgcgaacaag ttggcctgca aagcgcgaat aattcgccca
    20821 tcgacaaaag cctcttccgg gggattaatc agttccagct ttacgccgcg ttcacggcgc
    20881 aggacgttaa tatcgaaatt ttcaaaacga gacagcaatt ctttgctgtt atcggtcaaa
    20941 ctaccggagt tcagtacggc aagtgaacag ttgcgaaaca attgatagag gtcgctgctg
    21001 gcggtgtgtt taagcatatc cacttccagc tgcgacaaca tatccatgga gccaagcggg
    21061 ctaatatgtg taatcaagaa aaactcctta tgggacgaat atcgtcattc cctttgatta
    21121 caatagccct gcctgatgac ctttcacaac cttctgcgtg aatccagcac gcaaaattga
    21181 gaaaaaattt attgccgaac cagtcttgtg gtcgatggga caaaatcgct attactgcgc
    21241 cacgggttaa tgtccagacc gccacgacgg gtataacgtg cgtaaacgct caatttctct
    21301 ggctggcaga agcgtaacag gtcattaaag atgcgttcca cgcactgttc gtggaactcg
    21361 ttgtgatgac ggaatgagac caggtaacgc agcagttttt ctctgtcaat ttggcgtcca
    21421 cgatactgaa tttggatcga accccaatct ggttgatggg tgatcaggca gtttgatttc
    21481 agcaggtggc tgacaagcgt ctcttccact actttttcac cacaggtggc attctccaga
    21541 tagtcagtag tgaattcata gttatcgata gtgatatcct ggtcatcaat gcaagtgcca
    21601 ttgaaatgac ctatcggctg gccttccagt tcatcaagac gatataacgc cacgctaaca
    21661 ttaccctgag cgcaagtgct taagtcgcgc tccagcgtct ggcgcacctc atcccagtta
    21721 ttaaaacgcg tctggttaaa actgttgaga tagagcttaa aactcttcga ctcaatcaga
    21781 tttacgctgg tgtaatcaag ttcaacatga ccgacagcga cctgcggcaa acctttcgca
    21841 ttcagccagg aaagttcata cagcgtccag atgtccgcac cttgaaaagg caggttatcc
    21901 gctttcagac ccagcggatc gcgattcagg ctgcgtggaa cgccttgcag taggctggcg
    21961 tcataggtat cccggtaatc ggttgatttt ccaagagtca ggcccgcaag tgcctgatgg
    22021 tttgcataag aagacatgtt tcaccgtcat aaaccagata aactaaacga caagtgtatc
    22081 tggtttaagt gaagagagaa attagtggac gatttgaccg cacaagccct gaaagatttt
    22141 actgcacgct actgcgatgc gtggcatgaa gagcataaaa gctggccgtt aagtgaggaa
    22201 ctgtacggcg ttccttcacc gtgcattatt tcaactactg aagatgccgt atactggcaa
    22261 ccccagccgt ttaccgggga acaaaatgta aacgcggttg aacgcgcttt tgatattgtg
    22321 atacaaccca caattcatac cttttatacg actcagtttg ccggggatat gcacgcgcag
    22381 tttggcgata tcaaactgac attgctgcaa acctggagtg aagacgactt ccgtcgggtg
    22441 caggaaaatc tgattggtca tctggtgact cagaaaaggc taaaactgcc acccacgcta
    22501 tttatcgcga cgctggaaga agagttagag gtcatctcgg tgtgtaatct ctccggtgaa
    22561 gtgtgtaaag agacgctggg aacacgtaaa cggacgcatt tagcctcaaa tcttgcggaa
    22621 ttccttaatc aacttaagcc tcttctgtaa tccaaatacc ccatctggtt gtgagagatc
    22681 tcttacagac tctgtaggag atcgccagga aataagcgaa tacttaaaaa gataagaatc
    22741 gttattttca tttaaaaaca acatgttgct tgttaattcc taactttcat atgaaatttt
    22801 ccttaaggca ttgtctgtaa gcgccttgta agacttcgcg aaaaagacga ttctatcttc
    22861 gtcgacaggg agtcagacaa cgaagtgaac atcaggatga tgacacttct gaaggacaca
    22921 ccaggatggt gtttcaggga aaggcttctg gatgaagcga agaggatgac gcaggacgcg
    22981 ttaaaggaca cctccaggat ggagaatgag aaccggtcag gatgattcgg tgggtcagga
    23041 aggccaggga cacttcagga tgaagtatca catcggggtg gtgtgagcag gaagcaatag
    23101 ttcaggatga acgattggcc gcaaggccag aggaaaagtt gtcaaggatg agcagggagc
    23161 aacaaatgta gctggaatgc tgcgaaacga accgggagca ctgtgaatac agtgctccct
    23221 ttttttattc ctgctatcct tcgcggcagt ttttcttatt gaggttgctt tatgaccact
    23281 catgaccgcg ttcgtctcca gttgcaggcg cttgaagcgt tactgcgtga acatcagcac
    23341 tggcgaaacg atgtacccca gccgcatcaa tttaatagta cccaaccgtt ctttatggac
    23401 accatggaac cactggagtg gttgcagtgg gtgctgatcc cgcgaatgca cgatttgtta
    23461 aataacaacc agccgttacc gggcgctttt gccgttgctc cctattatga aatggcgctg
    23521 gcgacggacc atccgcaacg cgcgcttatt ctggctgagt tggaaaaact ggatgctctt
    23581 tttgcggatg atgcgagcta atgctggaaa tactctatca ggatgaatgg ctggttgcgg
    23641 taaataaacc ctccggctgg ctggttcacc gcagctggct ggatcgcgac gagaaagtag
    23701 tggtcatgca aaccgtgcgt gaccagatag gacagcatgt ttttactgct catcgtctgg
    23761 accgacccac ttctggtgtg ttgttgatgg gattatccag cgaggccgga cggctgctgg
    23821 cacaacagtt tgaacagcac caaatccaga aacgttacca tgcgattgtg cgcggctggt
    23881 tgatggaaga agcggtgctg gattatccac tggtggaaga actggacaaa atcgctgata
    23941 aatttgcccg cgaagataaa ggcccgcagc cagcagtgac gcattatcgc ggtctggcga
    24001 ccgtagaaat gccggtagcg accggacgtt acccgaccac gcgctacggc ctggtggaac
    24061 tggagccgaa aaccggacgc aaacatcagc ttcgccgaca tcttgcccat ttgcgtcatc
    24121 cgattattgg cgatagcaaa catggcgatt tacgccagaa tcgcagcggt gctgagcatt
    24181 ttggtcttca gcggttaatg ctgcatgcca gtcagttgtc attgacgcat ccttttactg
    24241 gcgagccgct gactattcac gcgggcctgg acgacacctg gatgcaggcg ttatcacaat
    24301 ttggctggcg cggtctgctt cctgaaaatg aaagggttga gttcagtgcg ccatcaggtc
    24361 aggatggaga gataagttcc taatccaggg agaaaacggt atggcggaaa ttggtatttt
    24421 tgtcggcacc atgtacggaa attcgctgtt agtggcggaa gaagcagaag caattctgac
    24481 cgcacagggc cacaaagcaa cggtatttga agatcctgaa ttaagcgact ggctgcccta
    24541 tcaggataag tatgttctgg tggttacgtc cacgaccggg cagggcgacc ttcctgatag
    24601 cattgtgccg ctctttcagg gaatcaaaga tagtctgggc ttccagccga atctgcgtta
    24661 tggcgtgatt gcgctcggcg acagcagtta tgtaaatttc tgcaatggcg gcaaacagtt
    24721 cgatgccttg ttgcaggaac agagcgctca gcgggttggc gaaatgctgt tgattgacgc
    24781 cagtgagaac ccggaaccgg aaacggaatc aaatccgtgg gtcgaacagt ggggcacgct
    24841 gttgtcctga tatgttcagc gagcggtaaa tgtcgtttta gcggtgctga atcgaatctt
    24901 ttcaggcaaa tgccagtaaa aactgcttca tagcgcggat ttttactggc gtttgcctgg
    24961 agtcaagcga tccatttcat actcttcttt atttcttcgt tttaaccctt cctttcttgt
    25021 tcttgttttc atttccgtga agtggattcc accgtccagg gctaatgcca aaatcgggcc
    25081 tcattgaacg cattaatgtt gtgttgttgc acggtgagcc gctatggcgc gctttttata
    25141 ctgctattgc cagatataaa cacgcgccgt attcggcgaa cgacctataa aaacggcaaa
    25201 aaacacccta cgtcacctct gatttcctgg cgatgtcgca gtccagagtg agcgtggcta
    25261 acgcgaattt tcaggagtgc aacaatgagt tctttaagtc aggctgcgag ccgtgtggaa
    25321 aaacgcacga atgctcgtta ctggatagtg gtgatgttgt ttatcgtcac atccttcaac
    25381 tacggcgacc gcgctacgct ttctattgcc ggttcggaaa tggccaaaga tatcggcctt
    25441 gatcccgtgg gaatgggcta tgtgttctct gccttctcat gggcttatgt tatcgggcag
    25501 atccctggtg gttggttgct ggatcgtttt ggttcaaaac gcgtctactt ctggtcgatc
    25561 tttatctggt cgatgtttac cttgctgcaa ggcttcgtcg atatctttag tggattcggc
    25621 attatcgttg ccctgtttac tctgcgcttc ctggtcgggc ttgctgaagc gccatctttc
    25681 cccggcaaca gtcgcattgt tgcggcctgg tttccggcgc aggaaagggg aacggcggtg
    25741 tcgattttta actccgctca atacttcgca acggtgatct tcgcgccgat tatgggctgg
    25801 ctgacgcatg aagtgggctg gtcacacgtc ttcttcttta tgggcggtct ggggattgtc
    25861 atcagcttta tctggttgaa agtcatccac gagccaaatc aacatccggg ggtaaatcag
    25921 aaagagctgg agtacatcgc cgcgggtggc gcgctgatca atatggatca gcaaaacacc
    25981 aaagttaaag tgccgttcag cgtgaagtgg gggcagatca aacagctgct cggatcacgg
    26041 atgatgatcg gcgtttatat cggtcagtac tgtatcaacg ccctgactta cttcttcatt
    26101 acctggttcc cggtttatct ggtgcaggcg cgcgggatgt cgattctgaa agcgggcttt
    26161 gtggcttccg ttccggcggt ttgcggtttt atcggcggtg tgctgggtgg gataatttcc
    26221 gactggctga tgcgccgcac ggggtcgctg aacattgcgc gtaaaacacc gatcgtaatg
    26281 ggcatgttgc tgtcgatggt gatggtgttc tgcaactacg tcaacgttga gtggatgatc
    26341 atcggcttta tggcgctggc cttcttcggt aagggcatcg gggcgctggg ttgggcagta
    26401 atggcagata ccgcgccaaa agagatcagc ggtctttccg gtggcctgtt caacatgttc
    26461 ggtaacattt ctggcatcgt cacgccaatc gcaattggtt atatcgttgg cacgactggc
    26521 tcgtttaatg gggcgctgat ttatgttggt gttcatgcct taatcgcggt actgagctac
    26581 ctggtgctgg tgggcgatat caagcgtatc gagctgaaac ctgtcgcggg gcaataagat
    26641 gacgacacaa tccagtcctg ttattactga tatgaaagtc attccggtcg ccgggcacga
    26701 tagcatgttg cttaatattg gtggggcaca taacgcatat ttcacccgca atattgtggt
    26761 actcaccgat aacgccgggc ataccggcat tggtgaagcg ccgggcggag aggtgattta
    26821 tcagacgctt gtcgaagcta ttccgatggt gctgggccag gaagtcgcgc gcctgaataa
    26881 agtggttcag caggtgcata aaggtaatca agcagccgat tttgatacct tcggcaaagg
    26941 tgcctggact tttgaattgc gcgttaatgc cgtagcggcg ctggaagccg ccttgcttga
    27001 cctgttaggt aaggcgctga atgttccggt ctgcgaactg ttagggccag gcaagcaacg
    27061 cgaggctatt accgtcctcg gttatctgtt ttatatcggt gatcggacca aaaccgatct
    27121 tccttatctg gaaaatacgc cgggcaacca tgagtggtat cagttgcggc atcagaaagc
    27181 gatgaacagc gaagccgttg tgcgtctggc ggaagcctca caggatcgct acggctttaa
    27241 agattttaaa cttaagggcg gcgtgttacc tggcgagcaa gaaatcgaca ctgttcgtgc
    27301 attgaagaaa cgcttcccgg atgcgcggat taccgttgat cccaacggtg catggctgct
    27361 tgatgaagcc atttctctgt gcaaagggct gaatgatgtt cttacctatg ccgaagatcc
    27421 atgcggcgca gaacagggct tctccggacg tgaagtgatg gcggagtttc ggcgggcgac
    27481 cggcttgccc gtcgcgacta acatgatcgc caccaactgg cgcgaaatgg gtcatgcggt
    27541 gatgctcaat gcggtagata ttccacttgc cgatccgcac ttctggacgc tttccggtgc
    27601 agtccgtgtg gcgcagcttt gcgacgactg ggggctgacc tggggctgcc attctaataa
    27661 ccatttcgat atctctctgg cgatgtttac ccatgtgggc gcggcggcgc cgggtaatcc
    27721 taccgctatc gatacccact ggatttggca ggagggcgat tgtcgcctga cccaaaatcc
    27781 gctggagatt aaaaacggaa aaattgccgt tcctgatgcg cccggtctgg gcgtggaact
    27841 ggactgggaa caggtacaaa aggcacatga ggcctataaa cgtctgcctg gcggtgcgcg
    27901 taacgacgca ggcccgatgc agtacctgat ccccggctgg acctttgacc gtaaacgtcc
    27961 cgttttcggc cgtcattgat tctgaaaaag gacataaata tgagttctca atttacgacg
    28021 cctgttgtta ctgaaatgca ggtcatcccg gtggcgggcc atgacagtat gctgatgaat
    28081 ctgagtggtg cacacgcacc gttcttcacg cgtaatattg tgattatcaa agataattct
    28141 ggtcacactg gcgtagggga aattcccggc ggcgagaaaa tccgtaaaac gctggaagat
    28201 gcgattccgc tggtagtggg taaaacgctg ggtgaataca aaaacgttct gacgctggtg
    28261 cgtaatactt ttgccgatcg tgatgccggc gggcgcggtt tgcagacatt tgatttgcgt
    28321 accactattc atgtagttac cgggatagaa gcggcaatgc tggatctgct ggggcagcat
    28381 ctgggggtaa acgtggcatc gctgctgggc gatggtcaac agcgtagcga agtcgaaatg
    28441 ctcggttatc tgttcttcgt cggtaatcgc aaagccacac cgctgccgta tcaaagccag
    28501 ccggatgact catgcgactg gtatcgcctg cgtcatgaag aagcgatgac gccggatgcg
    28561 gtggtgcgcc tggcggaagc ggcgtatgaa aaatatggtt tcaacgattt caaactgaaa
    28621 ggcggtgtac tggccgggga agaagaggcc gagtctattg tggcactggc gcaacgcttc
    28681 ccgcaggcgc gtattacgct cgatcctaac ggtgcctggt cgctgaacga agcgattaaa
    28741 attggtaaat acctgaaagg ttcgctggct tatgcagaag atccgtgtgg tgcggagcaa
    28801 ggtttctccg ggcgtgaagt gatggcagag ttccgtcgcg cgactggcct gccgactgca
    28861 accaatatga tcgccaccga ctggcggcag atgggccata cgctctcgtt acaatccgtt
    28921 gatatcccgc tggcggatcc gcatttctgg acgatgcaag gttcggtacg tgtggcgcaa
    28981 atgtgccatg aatttggcct gacctggggt tcacactcta acaaccactt cgatatttcc
    29041 ctggcgatgt ttacccatgt tgccgccgct gcaccgggta aaattactgc tattgatacg
    29101 cactggattt ggcaggaagg caatcagcgc ctgaccaaag aaccgtttga gatcaaaggc
    29161 gggctggtac aggtgccaga aaaaccgggg ctgggtgtag aaatcgatat ggatcaagtg
    29221 atgaaagccc atgagctgta tcagaaacac gggcttggcg cgcgtgacga tgcgatggga
    29281 atgcagtatc tgattcctgg ctggacgttc gataacaagc gcccgtgcat ggtgcgttaa
    29341 gacagaaaac caaaaccggc tctgtcagga gccggtttga gactgatgcc aaacacaaaa
    29401 ttgcctgatg cgctacgctt atcaggccta catgcttttt aatttattga atttaattgg
    29461 ttttgtaggc cggataaggc gttaacgccg catccggcat aaacacaggc actttgtcac
    29521 caatctgaaa ccagcgtcat aaaaagccga ttgctactcg acaagacgtc cgttacccga
    29581 gaattttgct ggcttcgcgc gcgacattat ccatctcgtc caacagttcc agcagctccg
    29641 gctccaaatc ttcttcttta gtaccgctac gtagctgttg ttcgatcagt tggcagagat
    29701 tcttcatacg cggcacaccg ctatagccgc aactgccatg cagtttatga atcaaatcaa
    29761 ccaggccttc cgggttttct ccaaccagct gttcctcaac tttgttgcga acttcaggca
    29821 ggaaatcgag taacatttgc agcatatcgc gcgctaaatc ggtttttcct gctgcctggc
    29881 gtagtgccag ttgccagtcg agggtcgcat tcgggttcac cacaatttcg ttgacttcgg
    29941 gcgtcacgac gcgagacgaa ataccgctgc caggcttgta gcgcaacaac aaattatgca
    30001 atcgctcttc ttcaatcggt ttcgccagat aatcgctcat tcctgcgcca agcagcttct
    30061 ctttttgccc ggccattgca tgcgccgtta ccgcgataac cggcgtttgt tgctgatggg
    30121 ggagctggtg gatgagctcg caggcccgaa tgccatccat gtccggcatt tgaatatcca
    30181 ttaagatcaa atcgaacggc atctgtttcg cccgttcaac cgcctgatgc ccgctatcgc
    30241 aaagttccac atgttgcacc atatcttcca gcaatgcgcc gataagtttc aggttagcgg
    30301 ggttgtcatc aaccgccatg actgtcattg ccagcttact ttcatcggtt acaggcaaaa
    30361 gcgtgttttg tttgtgatga caaaattccg tcagggcagg cagcaggcgc gtaggtgtta
    30421 atggtttcag cagacacgcg ccgataccat cttgcttgag tttttcagca ttgacttgtg
    30481 catggcaagg aagtgccagc atcaggaaat cggtcatcga taccgctttc gctaatcgct
    30541 catgttgcat tgttaacggc tcgcggaagg tcaccgcgat gcctaacaac atcatgtcgt
    30601 aatgcgcggg aggcagcgcg gagaacgttg ggctataaac cacttccagc ggcgtttcac
    30661 ttaaaatatc cagcgtgcat tgcgctgctg cggagtttgg ttcgacatag gccaggcgtt
    30721 taccggcgag gcactgggtg gatggccctt caataataat gttcgggttc agatcgagat
    30781 taatgtggaa ccagaaagtt gaaccgcgat ttggctggct atggaacgaa atatcgccgc
    30841 ccatttcatt aaccagtttt tgtgtaatca ccagccccag cccggtgcca ccatgacggc
    30901 gggaaatact ggcatcagcc tgtcggaagg cctggaataa gcgcgattga tcgcgttcag
    30961 gaataccaat gccggtatcc cgaatctgca cttcaatctg cactttggta ttactcagcg
    31021 cacgtttttc taccagaata tcaatgttgc cattctcggt gaatttaatt gcattcccca
    31081 ccaggttagt gatgatttgc tgtaatcgca gtgggtcgcc gatcacgtta tcaggcacgt
    31141 cgctcttaat attgagcgtc agttccagcc ctttatcgtg agaagaatga gccagcaggg
    31201 taacgacttc atccagcgtg ctgcgtagtg ggaatggaat actttccaga atcagcttac
    31261 ctgcttccag ttttgagaag tcgagaacat cattaattat tgccagcaaa ttatttgccg
    31321 aacgttcaat cgtattcagg tgatcgcgct gcgttggtgt taattctgtt ttcagcgtca
    31381 ggcgggtaaa gccaataaca ccattcagtg gtgtacgcag ctcgtgtgac atattagcca
    31441 gaaactcgga tttaatacgc gccgcttcct gggcgcgctt tttcgccaga tccaactcaa
    31501 cgttctgaat ttccatctgc tccagcgttt cacgcagatc ggacgtcgcc tggtcaatat
    31561 tgtgctgcat ctcttcgtga taagcagcca gcgacattgc catcgagttg ataccgtttt
    31621 tcagcatatc cagctcgccg agcataaatc cttccacccg gctgtcgagt tgcccgcgac
    31681 ggatgcggtc gacggtattc accatgttgc gaatcggacc ggttacatcg cgcattaagc
    31741 gccagccaaa aataagcgca ataccgatac aaaacagcat catcacgctg gaaataaaga
    31801 tctctttata ttgctgcaag cgaaccgatt taagatccag ctcaagcgca atatatccca
    31861 acatattttg actatttttg gcatcgctac tgggcgattc gtcaggggag taactctcag
    31921 aaataatcgg cgtgcggagg atcataatat cgccgtcacg agtgacagtg agctggcgag
    31981 gaaacggcac gttgctgccg agctgcattg atgagggatc aagatgaaaa ttggaggtga
    32041 caaagagtcg gttattttca tcataaaccg aaatcgcgcg aacaatatcg gaatggcgac
    32101 gatgcagtac gcttattaac tgaccgatag attcgcgatt ttgcaggctc atgccatatt
    32161 cagtagaaac tgcaagcggc tcaataatgc tggcaccggc atcttccagt tgacgctgca
    32221 agtcgttata gcgatgcacg acgaaaaaga tactcagcaa taaaccaata aggacggtcg
    32281 gtgccaggat cagaatcatc atgcgtgcgc gcaggctgta gttggtcatg gagttccgtt
    32341 atgggacaat taaggtcaca ctgttaaatt gagaaagtct cgccaatggc gcaattctac
    32401 tctgcaaaac gacgcacgac gacgcgtcag atcataaccg tttcagtcaa cgacctcgac
    32461 tcttttggtc agggcgtggc gcgacataac ggcaaaacgc tatttatccc cggattattg
    32521 ccgcaggaaa acgcggaagt tactgttact gaagataaaa aacagtattc ccgcgctaaa
    32581 gtcgtacgcc ggttaagcga tagcccggaa cgcgaaacgc cacgctgtcc tcattttggc
    32641 gtatgcggcg gctgtcagca acaacacgcc agcgtggatt tacagcagcg aagcaaaagt
    32701 gcggcactcg cccgattaat gaaacacgaa gtctctgaag tgatcgccga tgttccctgg
    32761 ggctatcgcc gtcgcgcgcg tttaagtctg aattacttac cgaaaacaca gcaacttcag
    32821 atggggtttc gcaaagcggg ctccagtgac attgtcgacg ttaaacaatg ccccatttta
    32881 gtgccccaac ttgaagcatt gctgcccaaa gtcagggcat gcctgggcag cttacaagct
    32941 atgcgccatc ttggtcatgt tgaactggta caggcaacca gcggcacgct gatgattttg
    33001 cgccataccg caccgctaag ttcggcagat cgcgaaaaac tggaacgctt ttcgcattct
    33061 gaaggcctgg atctgtatct cgcccccgat agtgagatac tcgaaaccgt ctctggtgag
    33121 atgccctggt atgactcaaa tgggttgcgc ttaactttta gcccgcgcga ttttattcag
    33181 gtcaatgcgg gtgtgaacca aaaaatggta gcgcgtgcgt tggaatggct ggatgtgcaa
    33241 cctgaagatc gcgtactgga tctgttctgc ggtatgggca actttacact gccattggcg
    33301 acacaagcag ccagtgtggt gggtgtagaa ggtgttcctg cgctggtgga aaaaggccag
    33361 cagaatgcgc gtcttaatgg cttacagaat gtgacgtttt atcatgaaaa tcttgaagaa
    33421 gatgtcacaa agcagccgtg ggcgaaaaac ggcttcgata aagtgttgct ggaccccgcg
    33481 cgagcaggtg ccgcaggtgt tatgcagcaa attataaaac tggaacctat tcgtatagtt
    33541 tatgtatcct gtaaccctgc aacgctggct cgggatagcg aagcgttatt aaaagcagga
    33601 tataccattg cgcgactggc gatgctggat atgttcccac acacgggaca tctggaatcg
    33661 atggtacttt tctcgcgcgt taaatagttg cgatttgccg atttcggcag gtctggtccc
    33721 taaaggagag gacgatggtt gcggtaagaa gtgcacatat caataaggct ggtgaatttg
    33781 atccggaaaa atggatcgca agtctgggta ttaccagcca gaagtcgtgt gagtgcttag
    33841 ccgaaacctg ggcgtattgt ctgcaacaga cgcaggggca tccggatgcc agtctgttat
    33901 tgtggcgtgg tgttgagatg gtggagatcc tctcgacatt aagtatggac attgacacgc
    33961 tgcgggcggc gctgcttttc cctctggcgg atgccaacgt agtcagcgaa gatgtgctgc
    34021 gtgagagcgt cggtaagtcg gtcgttaacc ttattcacgg cgtgcgtgat atggcggcga
    34081 tccgccagct gaaagcgacg cacactgatt ctgtttcctc cgaacaggtc gataacgttc
    34141 gccggatgtt attggcgatg gtcgatgatt ttcgctgcgt ggtcatcaaa ctggcggagc
    34201 gtattgctca tctgcgtgaa gtaaaagatg cgccggaaga tgaacgcgta ctggcggcaa
    34261 aagagtgcac caatatctac gcgccgttgg cgaaccgtct tgggattggg caactgaaat
    34321 gggagctgga agattactgc ttccgttatc tccacccgac cgaatacaaa cgcatcgcaa
    34381 aactgttgca tgaacgccgt ctcgaccgcg aacactatat cgaagagttt gtcggtcatc
    34441 tgcgcgctga gatgaaagct gaaggcgtta aagctgaagt gtatggtcgt ccgaaacaca
    34501 tctacagcat ctggcgcaaa atgcagaaaa agaacctcgc cttcgatgag ctgtttgatg
    34561 tgcgtgcggt acgtattgtc gccgagcgtt tacaggattg ttatgccgca ctggggatag
    34621 tgcacacaca ctatcgccac ctgccggatg agtttgatga ttacgtcgct aacccgaaac
    34681 caaacggtta tcagtctatt cataccgtgg ttctggggcc gggtggaaaa accgttgaga
    34741 tccaaatccg caccaaacag atgcatgaag atgcagagtt gggtgttgct gcgcactgga
    34801 aatataaaga gggcgcggca gctggcggcg cacgttcggg acatgaagac cggattgcct
    34861 ggctgcgtaa actgattgcg tggcaggaag agatggctga ttccggcgaa atgctcgacg
    34921 aagtacgtag tcaggtgttt gacgaccggg tgtatgtctt tacgccgaaa ggtgatgtcg
    34981 ttgatttgcc tgcgggatca acgccgctgg acttcgctta ccacatccac agtgatgtcg
    35041 gacaccgctg tatcggggca aaaattggcg ggcgcattgt gccgttcacc taccagctgc
    35101 aaatgggcga ccagattgaa attatcaccc agaaacagcc gaaccccagc cgtgactggt
    35161 taaacccaaa cctcggttac gtcacaacca gccgtgggcg ttcgaaaatt cacgcctggt
    35221 tccgtaaaca ggaccgtgac aaaaacattc tggctgggcg gcaaatcctt gacgacgagc
    35281 tggaacatct ggggatcagc ctgaaagaag cagaaaaaca tctgctgccg cgttacaact
    35341 tcaatgatgt cgacgagttg ctggcggcga ttggtggcgg ggatatccgt ctcaatcaga
    35401 tggtgaactt cctgcaatcg caatttaata agccgagtgc cgaagagcag gacgccgccg
    35461 cgctgaaaca gcttcagcaa aaaagctaca caccgcaaaa ccgcagtaaa gataacggtc
    35521 gtgtggtggt tgaaggtgtc ggtaacctga tgcaccacat cgcgcgctgc tgccagccga
    35581 ttcctggaga tgagattgtc ggcttcataa cccaggggcg cggtatttca gtacaccgcg
    35641 ccgattgcga acaactggcg gaactgcgct cccatgcgcc agaacgcatt gttgacgcgg
    35701 tatggggtga gagctactcc gccggatatt cgctggtggt ccgcgtggtg gctaatgatc
    35761 gtagtgggtt gttacgtgat atcacgacca ttctcgccaa cgagaaggtg aacgtgcttg
    35821 gcgttgccag ccgtagcgac accaaacagc aactggcgac catcgacatg accattgaga
    35881 tttacaacct gcaggtgctg ggtcgcgtgc tgggtaaact caaccaggtg ccggatgtta
    35941 tcgacgcgcg tcggttgcac gggagttagg ccgaaatttg ctcgtatcta caatgtagat
    36001 tgatatatac tgtatctaca tatgatagcg gtttgaggaa agggttatga tccacagtag
    36061 cgtaaagcgt tggggaaatt caccggcggt gcggatcccg gctacgttaa tgcaggcgct
    36121 caatctgaat attgatgatg aagtgaagat tgacctggtg gatggcaaat taattattga
    36181 gccagtgcgt aaagagcccg tatttacgct tgctgaactg gtcaacggca tcacgccgga
    36241 aaacctccac gagaatatcg actggggaga gccgaaagat aaggaagtct ggtaatggta
    36301 agccgatacg tacccgatat gggcgatctg atttgggttg attttgaccc gacaaaaggt
    36361 agcgagcaag ccggacatcg tccggctgtt gtcctgagtc cgttcatgta caacaacaaa
    36421 acaggtatgt gtctgtgtgt tccttgtaca acgcaatcaa aaggatatcc gttcgaagtt
    36481 gttttatccg gtcaggaacg tgatggcgta gcgttagctg atcaggtaaa aagtatcgcc
    36541 tggcgggcaa gaggagcaac gaagaaagga acagttgccc cagaggaatt acaactcatt
    36601 aaagccaaaa ttaacgtact gattgggtag tgttactaac tcacttctat tctggtcaca
    36661 ggtttccacc tgacggaccc gcaattttca ggacattcta atgaatcaaa tcgaccgttt
    36721 gctcactatt atgcagcgcc tgcgcgaccc ggaaaacggc tgcccgtggg ataaagagca
    36781 gacatttgcc accattgcgc cttacaccct tgaagaaacc tacgaagtgc tggacgccat
    36841 cgcccgtgaa gattttgacg atctgcgtgg tgaactcggc gatctgctgt tccaggtggt
    36901 gttttacgcc caaatggctc aggaagaagg gcgctttgac tttaatgata tttgcgctgc
    36961 gattagcgat aaattagagc gtcgccatcc gcatgttttt gctgacagtt ctgccgaaaa
    37021 cagtagtgaa gtgcttgccc gttgggagca aatcaaaacc gaagagcgcg cgcagaaagc
    37081 gcagcattcg gcgctggacg atattcctcg tagtttaccg gctttaatgc gggcgcaaaa
    37141 aatccagaaa cgttgcgcca acgttggctt cgactggacg acgcttggcc cagtggttga
    37201 taaagtctac gaagagatcg acgaggtgat gtacgaggca cggcaggctg ttatcgacca
    37261 ggctaaactg gaggaggaaa tgggtgacct gctgtttgcc acggtcaatc tggctcgtca
    37321 tttagggaca aaagcggaaa tcgcattgca aaaagcgaac gaaaaattcg agcgtcgttt
    37381 tcgcgaagtg gagcgtattg ttgccgcgcg tggactggaa atgacaggtg ttgacctcga
    37441 aacaatggaa gaagtctggc aacaggtaaa acggcaggaa attgatctct aagggaatta
    37501 cgcggtcaag cgccatttgt gtcatttttt aaatgacaag cgcttgattt gcgtcaaaaa
    37561 catttacccc aaaggggcta ttttctcact cctgatttca atagtgcgct ggcgaagagg
    37621 agggataatg aaagtttgtg gcacaggtca tgttcgggta tactgctttc ccgtcctggt
    37681 tattccatcg tcttttcaac ctaacttctc aggttcagca tgacaacgaa ctatattttt
    37741 gtgaccggcg gggtcgtatc ctctctgggt aaaggcattg ccgcagcctc cctcgcagcc
    37801 attcttgaag cccgtggcct caatgtgacc atcatgaaac tggatccgta catcaacgtc
    37861 gatccaggta ctatgagccc aatccaacac ggggaagtgt tcgttactga agacggcgct
    37921 gaaaccgacc tggacctggg gcactacgag cgtttcatcc gtaccaaaat gagccgccgc
    37981 aacaacttca ccacgggtcg tatctactct gacgttctgc gtaaagaacg ccgcggtgac
    38041 tacctcggcg caaccgtaca ggttattccg cacatcacta acgcaatcaa agagcgcgtg
    38101 ctggaaggtg gcgaaggtca tgacgtagta ctggtcgaaa tcggcggtac agtaggtgat
    38161 atcgaatccc tgccgttcct cgaagcgatt cgccagatgg ctgttgaaat tggccgtgag
    38221 cacactctgt ttatgcacct gacgctggtg ccgtacatgg cagcgtctgg tgaagtcaaa
    38281 accaaaccga ctcagcactc tgtaaaagag ctgctctcca tcggtatcca gcctgacatc
    38341 ctgatttgtc gttcagatcg cgccgttccg gcgaacgaac gtgcgaagat tgcattgttc
    38401 tgtaatgttc cggaaaaagc ggttatttct ctgaaagacg tcgattccat ctataaaatt
    38461 ccgggcctgt tgaaatctca ggggctggac gattatattt gtaaacgatt cagcttaaac
    38521 tgcccggaag cgaatctgtc cgaatgggaa caggttatct tcgaagaagc gaatccggta
    38581 agtgaagtca ccatcggtat ggtcggcaag tacattgaac tgccggatgc ttataaatcc
    38641 gtgatcgaag cactgaaaca cggtgggctg aagaatcgtg tcagcgtcaa catcaaactg
    38701 atcgattcac aagatgttga aacgcgcggc gttgaaatcc ttaaaggtct ggacgcaatc
    38761 ctcgtacctg gcggtttcgg ctatcgtggc gtagaaggca tgattacgac cgcgcgtttt
    38821 gcgcgtgaga acaatattcc ttatctgggc atttgcctgg gtatgcaggt ggcgttaatt
    38881 gattacgctc gccatgttgc caacatggag aacgccaact ctacggaatt tgtgccagac
    38941 tgtaagtacc cggttgtggc gctgattacc gagtggcgcg atgaaaacgg caacgttgaa
    39001 gttcgtagcg agaagagcga tctcggcggt accatgcgtc tcggcgcaca gcagtgccag
    39061 ttggttgacg atagcttggt tcgccagctg tacaatgcgc cgacaattgt tgagcgtcat
    39121 cgtcaccgtt acgaagtcaa caacatgctg ttgaaacaga ttgaagatgc aggtctgcgc
    39181 gttgcgggcc gttccgggga tgatcagttg gtcgagatca tcgaagttcc gaatcacccg
    39241 tggttcgtgg cttgccagtt ccatccggag tttacttcta ctccacgtga tggtcacccg
    39301 ctgtttgcag gctttgtgaa agccgccagc gagttccaga aacgtcaggc gaagtaagta
    39361 aaaaagttag aacggcaacg cgtaccctgg gtacgcgttg tttgtctgga gtttcagttt
    39421 aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa aaatcatcgg tcgtgaaatc
    39481 atcgactccc gtggtaaccc gactgttgaa gccgaagtac atctggaggg tggtttcgtc
    39541 ggtatggcag ctgctccgtc aggtgcttct actggttccc gtgaagctct ggaactgcgc
    39601 gatggcgaca aatcccgttt cctgggtaaa ggcgtaacca aagctgttgc tgcggtaaac
    39661 ggcccgatcg ctcaggcgct gattggcaaa gatgctaaag atcaggctgg cattgacaag
    39721 atcatgatcg acctggacgg caccgaaaac aaatccaaat tcggcgcgaa cgcaatcctg
    39781 gctgtatctc tggctaacgc caaagctgct gcagctgcta aaggtatgcc gctgtacgag
    39841 cacatcgctg aactgaacgg cactccgggc aaatactcca tgccggttcc gatgatgaac
    39901 atcatcaacg gtggtgagca cgctgacaac aacgtcgata tccaggaatt catgattcag
    39961 ccggttggcg cgaaaactgt gaaagaagcc atccgcatgg gttctgaagt tttccatcat
    40021 ctggcaaaag ttctgaaagc gaaaggcatg aacactgcag ttggtgacga aggtggctat
    40081 gcgccgaacc tgggttccaa cgctgaagct ctggctgtta tcgctgaagc tgttaaagct
    40141 gctggttatg aactgggcaa agacatcact ctggcgatgg actgcgcggc ttctgaattc
    40201 tacaaagacg gtaaatacgt tctggctggc gaaggcaaca aagctttcac ctctgaagaa
    40261 ttcactcact tcctggaaga actgaccaaa cagtacccga tcgtttctat cgaagacggt
    40321 ctggacgaat ctgactggga cggtttcgca taccagacca aagttctggg cgacaaaatc
    40381 cagctggttg gtgacgacct gttcgtaacc aacaccaaga tcctgaaaga aggtatcgaa
    40441 aaaggtatcg ctaactccat cctgatcaaa ttcaaccaga tcggttctct gaccgaaact
    40501 ctggctgcaa tcaagatggc gaaagacgct ggctacactg cagttatctc tcaccgttct
    40561 ggcgaaactg aagacgctac catcgctgac ctggctgttg gtactgctgc aggccagatc
    40621 aaaactggtt ctatgagccg ttctgaccgc gttgctaaat acaaccagct gattcgtatc
    40681 gaagaagctc tgggcgaaaa agcaccgtac aacggtcgta aagagatcaa aggccaggca
    40741 taagtctgac tttatctgat ttaaaaatgc cagcccggag gctggcattt tttatggttg
    40801 agttaccagc ggccggatgc gccgccacct ccagaagaac cgcccccgcc gctaaatccg
    40861 ccgccacttg agcctccacc ggaagaggat gaagaagaac cttttcttga agctctttct
    40921 ttactgagtt ttttgtattt ggcgcgtagc tttttattga cgataagcca aaaaataacc
    40981 agtggtacga tcgcaaaagg cgttaacata ccgcaggcaa cgattaaatt ggtccatggt
    41041 cctggctcgg caacatagag gaataccatc ccgtaaatgg cgccagtgag agtgatccag
    41101 ggtaaattcg acggatcggt aaaaacaata aaatatgaaa gtaccgccag caatataatt
    41161 acagcaagcg ataatggaat agggggattg atactgtaat aatcccccct gtcttccggt
    41221 aattcaccat tcaacagaag cgcgccgata ttttcgcttg ccagtgttaa tccacccatc
    41281 aggtcatcac ttttgaaggc agggatcaat atatccctga taatcttcgc cgcttgcagg
    41341 tcggtaacca ccccttccag cccgtagcca acctcaatac gaacagcatg atcctcccag
    41401 gcaaccagaa gtaaaatacc atcgtttctc tgcttatctc ccagtttcca gctatcaaaa
    41461 acccttgtag catattgttc aatggaatcg tctccggtgg acggcactac taatacagcg
    41521 acctgcgccc gtgttttatg tgtaatatcc tgcagctgct gtgtcagtga ttgttgttca
    41581 gacgttctta acgtaccagt aatatcagtc acttgttggc gcagctccgg cacagcaatt
    41641 tgctgggcaa gcccgggaag gctccacaaa gcaagtagaa taaagattaa gcgcattacg
    41701 atcaccaacg gccggatgaa ccgccgcctc ctgaagaacc gccaccaccg cggaaagaat
    41761 ttctcgacga agagcgagag tgagcattac gattccccgt tttcctgttg cttttttctc
    41821 ctgatcccag cttaagattg ccagtaaatt tggccacaat gatcagcaga acgatgccag
    41881 caatggttat tggaccgata aaatgatcaa ttaacccaat aattgttgag atgaatgcaa
    41941 tatctttaat actggcgaac accaggcacc atcccacaat aattatcagc atcacgacaa
    42001 acaatgaatt tcgtcctgat tcacgaacct gcggagtctt cttttgtttt ttcactttag
    42061 cgtttttatt ttttggggcc agcatcatag gaaatacgcg gcacatatta aaaactaccg
    42121 cgagacgctg cttgcaggtt gttgctaaca ataaactaaa acaaacgacc gatacccgcc
    42181 gaatgatgac atcgtcctga aaggagaaaa accagaccat aggggtaatc atcgatatca
    42241 tccatatgcc acgccgtggg cggctggcaa atcgccagag gacaacgatg gcaacgatca
    42301 tcagcagtgt ccaggcaaga catggcagcg aacgttgcgg attagcaaac agatgattgg
    42361 ctaaagcatc caaagggcta aggttcgctt tgatatgggt aaactgtgcg atgctttcaa
    42421 ttccgcccgt taatccagca aagagattgt ttttttcgaa ttctggctgc atatggtaac
    42481 gaacaatttg cgcagcttgt ttcggcggca acatctcttc cagtccatgt ccaatcttca
    42541 ttgacgccag cccttccggc caaacgacga gaattaagat accttcgccg tatcgcttct
    42601 ctccaatggg tttccagtga tataacccgt agctctgcga ggctgcaagt ttaatgtttt
    42661 tgccccctgt ggttggcgta actaacacgg ccacctgtaa gtggttttgc tgctcgagag
    42721 tctgtaattg ttgatttaaa acactttgtt cttctgtcgt taatgtcccg gttaagtcat
    42781 tcactgaagg caatcgttca gcgaatacct gaaaactgca tagcatcaga agtaacagaa
    42841 aaatacgcat atcaattcct tttgttacca gcgcccggaa gcgccaccgc caccagatga
    42901 accgccacca ccgctgaaat tgtcattgtt gaatgaagag gatgacgatg atgaatagtg
    42961 ggaggaagaa ttgcttcttg tatggcgtga tggctggttg gcatcacgca tcaaatcccg
    43021 aacttttttg ccgaaggggg taaaagcgat ggcgacagcc aggaacggtg tggcgaagca
    43081 gagtaatgtt gtgagtactc cccagcttcc gctgaaacct gctataggta aaacaaaggc
    43141 cagtacaata gctgcgaagc aaattactcc cagcgttttg atatatcctc tggcagaaat
    43201 aaacgtcagt actatcgccc aggctaacaa ggcccaccaa ccgctaaaag gtaaagggtg
    43261 atcggccggt gctatcgtcg ctaaattatt ccccgtaaga cgactttcaa tatcgctgat
    43321 ccccttttgt aaaccgccag ccaggtcgcc ttttttaaat gcaggaataa tactgttgcg
    43381 aataattttc ccggactgcg catccgtaat aataccttcc agtccgtaac cgatttcaat
    43441 acgcacggta tgatcctgcc aggcgactaa cagtagaacg ccatcatcgc ggtctttatc
    43501 acccggtttc cagctatcga atacccgcgt tgcgtactgc tcgatggttt catcgcctgt
    43561 tgtttcaacc aataacacga ccagttgtgc gtggcttttt tgcgtcaggg ctttaatctg
    43621 gttggtcagg gtgtttactt cgctgtttgt taatgtgccc gttaagtcat tcacatactt
    43681 gggtgattca ggtactggaa tttgtgtggc aaacacagat gaacagaaga aagccataag
    43741 gaagataaaa tttcgcatag aacttccccg cttaatgagc attagcattc tggctaaaat
    43801 ctattgttgg ggctttactt actgcggcga catcatccgg caaataattt ttctttggtg
    43861 tgtaatccat gacctttgcg gttaacacag cggggaattt gcggatagtg acattatatt
    43921 gttcaatggc tttaatatat cttcctcgcg caacggcaat ccgattttca cttccttcaa
    43981 gctgtaccat taaattttga tagagttcct gagattttaa ctcagggtaa cgctcactaa
    44041 ttatcgttaa ctgaccgaga gtgcgagtca cctgcgcttg agcctgttgc caggcttgta
    44101 atttttgttc atctcccgga gtttgctgaa gatcgctgct ggcgcgatta gcctggctac
    44161 gagccaatgt tacggcttcc agtacatctc gttcatggct ggaataacct ttaatgctgg
    44221 cgacaagatt aggaataaga tcggcgcgac gttgatattg attcaatacc tcagaccagg
    44281 aggcgttaac ctgctcatca taggtttgaa tatcgttata tccacagcct gataaattga
    44341 aaataaatat aattacgatg aatattctga gcatgtttaa atccctttta attaattcct
    44401 taaaacataa tactctgctt cattttattc tttatgatct aatccacttt ttgccataaa
    44461 cctgcctgta acagaatgtt aataagacat gattgttgtg gcaggatctg caataattaa
    44521 caattatcca cttcacagag aatgctatgc agtacccgat taacgagatg ttccagaccc
    44581 tgcaaggtga gggttacttt accggcgtcc ccgccatttt tattcgttta cagggatgcc
    44641 cggttggctg tgcctggtgc gacaccaaac acacctggga aaagcttgag gatcgggaag
    44701 tctccctttt cagcattctg gcgaagacca aagagagtga taagtggggg gcggcgagca
    44761 gtgaagactt gctggctgtc attggtcgcc agggatatac cgcgcggcat gtggtgatta
    44821 cgggtggtga gccttgcatt catgatttgc tgccactgac tgacctgctc gaaaagaacg
    44881 gttttagctg ccagattgaa accagtggta ctcatgaagt acgctgtaca ccgaatacct
    44941 gggttaccgt atcgccaaag ctgaacatgc gcggcggcta tgaagtgttg tcacaggcac
    45001 tggagcgagc caacgaaatc aagcatccag tgggacgcgt acgtgatatt gaagcactgg
    45061 atgaactttt ggcgacgctg accgatgata aaccgcgagt cattgcactg cagccgatta
    45121 gccaaaagga tgatgccaca cgtttgtgca ttgaaacctg cattgcgcgt aattggcgtt
    45181 tgtcgatgca aacccataaa tatttaaata ttgcctgatt aaacatttat aagcgttata
    45241 aatgggtgga gcttgtaaag aattccactc atttgtattc tttgtcgtct ctgaaaaacc
    45301 tccattttgc ccatcctgaa ctaatcatta tcattctcta caaattctgt ggcgttaatt
    45361 tttcgctgga gtgaaaatta ttgcggtaaa gtttggtaga ttttagtttg tatagagtta
    45421 ttttaaatat ttaccttttt aatcaattgg ttaagtgctc tttaacataa tggatgtgtt
    45481 gtttgtgtga tactgtaaag ttggtagatt gtgactggct taaaaaatca ttaattaata
    45541 ataggttatg tttagagagt tccccgcgcc agcggggata aaccgggcgc ttcggtgtcg
    45601 atctgggcgt tgatggcgag ttccccgcgc cagcggggat aaaccgatgt cggctgcaat
    45661 ctgccgcgcc gggcgtgtga gttccccgcg ccagcgggga taaaccggct ggatacagtc
    45721 tggcagggtt ctgtgatgag agttccccgc gccagcgggg ataaaccgat gatgcagccg
    45781 tctcaggacg accttcgcac gagttccccg cgccagcggg gataaaccga acggctacgg
    45841 cactgaggtg acaaaccaga tgagttcccc gcgccagcgg ggataaaccg gtttctggca
    45901 tcggaaaaca ttgcatatcg t
//
LOCUS       DAZGCQ010000030        45063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000030 DAZGCQ010000000
VERSION     DAZGCQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.030"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            365..739
                     /gene="tomB"
                     /locus_tag="ACHK07_001805"
     CDS             365..739
                     /gene="tomB"
                     /locus_tag="ACHK07_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO8102147.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            765..983
                     /gene="hha"
                     /locus_tag="ACHK07_001806"
     CDS             765..983
                     /gene="hha"
                     /locus_tag="ACHK07_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO8102148.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1155..1706
                     /gene="maa"
                     /locus_tag="ACHK07_001807"
     CDS             1155..1706
                     /gene="maa"
                     /locus_tag="ACHK07_001807"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO8102149.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1822..2292
                     /gene="ylaC"
                     /locus_tag="ACHK07_001808"
     CDS             1822..2292
                     /gene="ylaC"
                     /locus_tag="ACHK07_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO8102150.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2456..4006
                     /gene="pdeB"
                     /locus_tag="ACHK07_001809"
     CDS             2456..4006
                     /gene="pdeB"
                     /locus_tag="ACHK07_001809"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO8102151.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4048..4401)
                     /gene="ybaA"
                     /locus_tag="ACHK07_001810"
     CDS             complement(4048..4401)
                     /gene="ybaA"
                     /locus_tag="ACHK07_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO8102152.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4521..4617)
                     /gene="ffs"
                     /locus_tag="ACHK07_001811"
     ncRNA           complement(4521..4617)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK07_001811"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4780..5091
                     /locus_tag="ACHK07_001812"
     CDS             4780..5091
                     /locus_tag="ACHK07_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO8102153.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5122..5694)
                     /gene="ybaY"
                     /locus_tag="ACHK07_001813"
     CDS             complement(5122..5694)
                     /gene="ybaY"
                     /locus_tag="ACHK07_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO8102154.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5912..6772
                     /gene="tesB"
                     /locus_tag="ACHK07_001814"
     CDS             5912..6772
                     /gene="tesB"
                     /locus_tag="ACHK07_001814"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO8102155.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKKMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6821..8107)
                     /gene="amtB"
                     /locus_tag="ACHK07_001815"
     CDS             complement(6821..8107)
                     /gene="amtB"
                     /locus_tag="ACHK07_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO8102156.1"
                     /translation="MKIATIKTGLASLAMLPGLVIAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8137..8475)
                     /gene="glnK"
                     /locus_tag="ACHK07_001816"
     CDS             complement(8137..8475)
                     /gene="glnK"
                     /locus_tag="ACHK07_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO8102157.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8656..10437)
                     /gene="mdlB"
                     /locus_tag="ACHK07_001817"
     CDS             complement(8656..10437)
                     /gene="mdlB"
                     /locus_tag="ACHK07_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8102158.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10430..12202)
                     /gene="mdlA"
                     /locus_tag="ACHK07_001818"
     CDS             complement(10430..12202)
                     /gene="mdlA"
                     /locus_tag="ACHK07_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8102159.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12232..12690)
                     /gene="decR"
                     /locus_tag="ACHK07_001819"
     CDS             complement(12232..12690)
                     /gene="decR"
                     /locus_tag="ACHK07_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO8102160.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12844..13662)
                     /gene="cof"
                     /locus_tag="ACHK07_001820"
     CDS             complement(12844..13662)
                     /gene="cof"
                     /locus_tag="ACHK07_001820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO8102161.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13762..15462
                     /gene="ybaE"
                     /locus_tag="ACHK07_001821"
     CDS             13762..15462
                     /gene="ybaE"
                     /locus_tag="ACHK07_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO8102162.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15527..16222
                     /gene="queC"
                     /locus_tag="ACHK07_001822"
     CDS             15527..16222
                     /gene="queC"
                     /locus_tag="ACHK07_001822"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO8102163.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLTDKP
                     TVMAAMKQKTGLK"
     gene            complement(16274..16672)
                     /gene="fadM"
                     /locus_tag="ACHK07_001823"
     CDS             complement(16274..16672)
                     /gene="fadM"
                     /locus_tag="ACHK07_001823"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO8102164.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16778..17149)
                     /gene="ybaV"
                     /locus_tag="ACHK07_001824"
     CDS             complement(16778..17149)
                     /gene="ybaV"
                     /locus_tag="ACHK07_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO8102165.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17300..19171)
                     /gene="ppiD"
                     /locus_tag="ACHK07_001825"
     CDS             complement(17300..19171)
                     /gene="ppiD"
                     /locus_tag="ACHK07_001825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8102166.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDKVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19363..19653)
                     /gene="hupB"
                     /locus_tag="ACHK07_001826"
     CDS             complement(19363..19653)
                     /gene="hupB"
                     /locus_tag="ACHK07_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO8102167.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19844..22198)
                     /gene="lon"
                     /locus_tag="ACHK07_001827"
     CDS             complement(19844..22198)
                     /gene="lon"
                     /locus_tag="ACHK07_001827"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO8102168.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22386..23660)
                     /gene="clpX"
                     /locus_tag="ACHK07_001828"
     CDS             complement(22386..23660)
                     /gene="clpX"
                     /locus_tag="ACHK07_001828"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO8102169.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23786..24409)
                     /gene="clpP"
                     /locus_tag="ACHK07_001829"
     CDS             complement(23786..24409)
                     /gene="clpP"
                     /locus_tag="ACHK07_001829"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO8102170.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGKSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24655..25953)
                     /gene="tig"
                     /locus_tag="ACHK07_001830"
     CDS             complement(24655..25953)
                     /gene="tig"
                     /locus_tag="ACHK07_001830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO8102171.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFSIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26297..26614)
                     /gene="bolA"
                     /locus_tag="ACHK07_001831"
     CDS             complement(26297..26614)
                     /gene="bolA"
                     /locus_tag="ACHK07_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO8102172.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26919..27497
                     /gene="yajG"
                     /locus_tag="ACHK07_001832"
     CDS             26919..27497
                     /gene="yajG"
                     /locus_tag="ACHK07_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8102173.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27541..29016
                     /gene="ampG"
                     /locus_tag="ACHK07_001833"
     CDS             27541..29016
                     /gene="ampG"
                     /locus_tag="ACHK07_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO8102174.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            29475..30422
                     /gene="cyoA"
                     /locus_tag="ACHK07_001834"
     CDS             29475..30422
                     /gene="cyoA"
                     /locus_tag="ACHK07_001834"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO8102175.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANDPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            30444..32435
                     /gene="cyoB"
                     /locus_tag="ACHK07_001835"
     CDS             30444..32435
                     /gene="cyoB"
                     /locus_tag="ACHK07_001835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO8102176.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            32425..33039
                     /gene="cyoC"
                     /locus_tag="ACHK07_001836"
     CDS             32425..33039
                     /gene="cyoC"
                     /locus_tag="ACHK07_001836"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO8102177.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMAPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            33039..33368
                     /gene="cyoD"
                     /locus_tag="ACHK07_001837"
     CDS             33039..33368
                     /gene="cyoD"
                     /locus_tag="ACHK07_001837"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO8102178.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            33380..34270
                     /gene="cyoE"
                     /locus_tag="ACHK07_001838"
     CDS             33380..34270
                     /gene="cyoE"
                     /locus_tag="ACHK07_001838"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO8102179.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            34419..35783
                     /gene="yajR"
                     /locus_tag="ACHK07_001839"
     CDS             34419..35783
                     /gene="yajR"
                     /locus_tag="ACHK07_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8102180.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCIGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(35911..36402)
                     /gene="yajQ"
                     /locus_tag="ACHK07_001840"
     CDS             complement(35911..36402)
                     /gene="yajQ"
                     /locus_tag="ACHK07_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO8102181.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            36570..37481
                     /gene="panE"
                     /locus_tag="ACHK07_001841"
     CDS             36570..37481
                     /gene="panE"
                     /locus_tag="ACHK07_001841"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO8102182.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            37444..38034
                     /gene="yajL"
                     /locus_tag="ACHK07_001842"
     CDS             37444..38034
                     /gene="yajL"
                     /locus_tag="ACHK07_001842"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO8102183.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(38088..39536)
                     /gene="thiI"
                     /locus_tag="ACHK07_001843"
     CDS             complement(38088..39536)
                     /gene="thiI"
                     /locus_tag="ACHK07_001843"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO8102184.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            39742..39984
                     /gene="xseB"
                     /locus_tag="ACHK07_001844"
     CDS             39742..39984
                     /gene="xseB"
                     /locus_tag="ACHK07_001844"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO8102185.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39984..40883
                     /gene="ispA"
                     /locus_tag="ACHK07_001845"
     CDS             39984..40883
                     /gene="ispA"
                     /locus_tag="ACHK07_001845"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO8102186.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            40908..42770
                     /gene="dxs"
                     /locus_tag="ACHK07_001846"
     CDS             40908..42770
                     /gene="dxs"
                     /locus_tag="ACHK07_001846"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO8102187.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            42825..43799
                     /gene="yajO"
                     /locus_tag="ACHK07_001847"
     CDS             42825..43799
                     /gene="yajO"
                     /locus_tag="ACHK07_001847"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO8102188.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEDLGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            44030..>45063
                     /gene="espY3"
                     /locus_tag="ACHK07_001848"
     CDS             44030..>45063
                     /gene="espY3"
                     /locus_tag="ACHK07_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO8102189.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKT"
BASE COUNT        10763 a        11856 c        11089 g        11355 t
ORIGIN      
        1 taatccataa tcatttagag gctattctta attatttgcg gtaattcttt attcattcct
       61 cggttattac gtcatattca gagcaatcct ggtattagtg tcaccaattt catctggcga
      121 taatcctgaa atgttatgaa cagttcgagc aaactgcttt tacctgctgc gggttggtgc
      181 tagtatgaaa aagtgagccc tgtcccgctt ccttcctaat tgtaattttt cgtaataatg
      241 cgatgaaaac ctgcaaagag tggcttatag ttaagctaac aaacgagagg gcaagtccag
      301 gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt caaccgctaa gaaggggacg
      361 cgttatggat gaatactcac ccaaaagaca tgatatcgca cagcttaagt ttctctgtga
      421 aactctgtat catgactgcc ttgcaaacct tgaagaaagc aatcatggct gggttaacga
      481 cccaacctcg gcgatcaacc tccagttgaa tgaactgatt gagcatattg cgaccttcgc
      541 acttaattac aaaattaagt ataatgaaga caataagctc attgagcaga tcgacgaata
      601 tctggatgac acctttatgt tgttcagcag ttatggtatt aatatgcagg atcttcagaa
      661 atggcggaag tcaggtaatc gactattccg ttgttttgtc aatgcgacga aagagaatcc
      721 tgcgagttta tcttgttaga attattacaa ccatgggtag aagtatgtcc gaaaaacctt
      781 taacgaaaac cgattattta atgcgtttac gtcgttgcca gacaattgac acgctggagc
      841 gtgttatcga gaaaaataaa tacgaattat cagataatga actggcggta ttttactcag
      901 ccgcagatca ccgcctcgcc gaattgacca tgaataaact gtacgacaag atcccttcct
      961 cagtatggaa atttattcgc taataaataa ttcgctttcg gagctataac cggctgttta
     1021 ttaagaattt tatacttttt cgccatgaag acatacccta tgtgatcttt atcacacaga
     1081 tgtaatggga acgttctctt cactgacttt tcgtcttact gtgttgccgc attttcagca
     1141 accggaggtc agtaatgagc acagaaaaag aaaagatgat tgctggtgag ttgtatcgct
     1201 cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg tcagcttatt caccgataca
     1261 atcattccct ggcggaagag cacacattac gccagcaaat tctcgctgat ttattcggtc
     1321 aggtgacaga ggcttatatt gagccaacgt ttcgctgtga ctatggctat aacatttttc
     1381 tcggtaataa ttttttcgcc aacttcgatt gcgtgatgct tgatgtctgc cctattcgca
     1441 tcggtgataa ctgtatgttg gcaccaggcg ttcatatcta cacggcaaca catcccatcg
     1501 accctgtagc acgtaatagc ggtgctgaac tggggaaacc cgtcaccatc ggtaataacg
     1561 tctggattgg cggacgcgcg gtcattaacc ctggtgtgac cattggtgat aacgtcgtgg
     1621 tagcctcagg tgcagttgtc acaaaagatg tcccggacaa cgttgtcgtg ggcggtaatc
     1681 cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa ctgttatgca aaattgtggt
     1741 aaatctgtta ctttccctct actattccca cgttaaaata gggtgttccc tggaaagttg
     1801 cagataccac gaaggcaaac gatgaccgaa atacaacgcc tgctgaccga aacgattgag
     1861 tctctgaata cccgcgaaaa acgcgacaac aaaccccgct ttagtatcag ttttatccgt
     1921 aaacatccgg ggctgtttat cggtatgtac gttgcttttt ttgccaccct ggcggtgatg
     1981 ttgcagtccg aaacgctttc aggctctgtc tggctactgg ttgtattatt tatcctgctt
     2041 aatggtttct tctttttcga tgtctaccca cgctaccgct atgaagatat cgacgtgctg
     2101 gatttccgcg tttgctataa cggcgaatgg tacaacacgc gctttgtacc tgccgcgctg
     2161 gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca actgcaaaaa
     2221 atgatcgtcc gtaaaggtga attgtctttt tacgatattt ttaccctcgc tcgcgccgaa
     2281 tcaacatctt aagttaggat tacataccag gcgtaaagct ctgcgcctgg tcaaatgaca
     2341 acgatcgttt ccacccatca cttcatgaaa taccagctct acctccttat ctcctgccag
     2401 cctttttcca caatcagata tactttccct acactgtgtt aataaggata tgctggtgag
     2461 aacacgacat ctggtcggcc ttatttcggg agtactgatt ctttcagtat tgctgcctgt
     2521 cggcttaagc atctggctgg cccatcagca ggtagaaaca tcgtttattg aagagctgga
     2581 tacctattcc tcccgcgtcg ctattcgggc caataaggtg gcgacacaag ggaaagatgc
     2641 gctgcaagag ctggaaagat ggcaaggcgc tgactgtagc gaagcccatc tcatggaaat
     2701 gcgtcgggta tcttacagtt atcgctatat tcaggaagtg gtttatatcg ataacaacgt
     2761 tccccaatgt tcgtctctgg agcatgagag tccgcccgat accttccccg agccaggtaa
     2821 aatttcgaaa gatggttatc gtgtctggtt aacatcgcat aacgatttag gcattatccg
     2881 ttacatggtc gccatgggaa cggcacatta tgtcgtcatg atcgaccccg cttcctttat
     2941 tgatgtcatt ccctatagct catggcaaat tgatgccgcc attattggca atgcccataa
     3001 tgttgtcata accagcagcg atgaaattgc tcagggaatt attaccaggc tacaaaaaac
     3061 acccggtgag catatcgaaa ataatggaat catttacgat atcctgccct tcccggagat
     3121 gaatatttcg atcatcacct gggcttcaac gaaaatgttg cagaaaggct ggcatcggca
     3181 agtctttatt tggttaccgc tcgggttggt gattggcctg ctggcagcga tgtttgtgct
     3241 gcgtattttg cgccgcatcc agtcaccgca tcatcggctg caggatgcta tcgaaaatcg
     3301 tgatatttgc gtgcactatc agccgattgt ctccttagcc aatggcaaaa ttgtcggtgc
     3361 tgaggcactg gcgcgctggc cgcagacaga cggcagttgg ttgtcaccag acagttttat
     3421 tccgctggca cagcaaacgg gcctttctga gccattgacg ctactgatta taagaagcgt
     3481 ctttgaagat atgggcgact ggctgcgtca gcatccacag cagcatattt cgatcaatct
     3541 tgaatccacc gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca
     3601 ctatcaggtt aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc
     3661 gaaaaccagc gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga
     3721 tgattttggt acggggtatt caagtttaag ttatttacag gatctggatg tcgacattct
     3781 gaagatcgat aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat
     3841 cgaaatggca aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca
     3901 agaagagtgg ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc
     3961 attaccgaaa gaagatttct tacgctgggc cgagcaacat ttgtgaattt ttgggctgac
     4021 ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg attgactcga atccgccata
     4081 gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc gtgggtccga
     4141 catcatcttt tgattagctg cgtcgcggac ctcttttgaa gggtattcaa tccagctaaa
     4201 gacaacctct tcattctctt ccgctttcac cgccatacga aaatcggtca ctttgccatc
     4261 cggtacatcg ctggcccagc attcgacaat acgaagcgcg ccaaactctt taaacaatgg
     4321 cgcggcctta gcggccattt cccgataggc atccttttta tcggcaggaa cggcaaccac
     4381 aaaaccatca acatacttca tcagacatac ctccgaagcg tactgcgcag ccagacaatt
     4441 cttgcgacgt cagttgtggt taaagtttag tcacgctggg attttttgac gaaacggtgg
     4501 gaagcagaaa tgggtggggg ccctgccagc tacatcccgg cacacgcgtc atctgccttg
     4561 gctgcttcct tccggacctg acctggtaaa cagagtagcg ttgcgggaga accaacagag
     4621 cccccattga gagcgttgag aaccaacgcg caggcgcatt atcactgctg ccccggctaa
     4681 ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa tgcgacttca ctcgggcgtt
     4741 tttccagatt atgctgaaaa attacctcag gaagaaaaga tggaaaaaga agattcattt
     4801 ccccaacgcg tctggcaaat cgtcgccgct attcccgaag gctatgtaac cacttacggt
     4861 gatgtggcga aactggcggg atcgccccgc gccgcgcgcc aggtgggcgg tgtgttaaag
     4921 cgtctccctg aaggcagcac cttaccctgg caccgggtgg ttaatcgcca cggcacaatt
     4981 tcgctaaccg gaccggattt acagcgtcag cgacaggcat tactggcaga aggtgtgatg
     5041 gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct ggaactacta accctctccg
     5101 ggcggagagg gttaacgtcg cttaatattg cgtaggagct ggaactgccg aagacggatt
     5161 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
     5221 cacggcagtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
     5281 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
     5341 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
     5401 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
     5461 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
     5521 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
     5581 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
     5641 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
     5701 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
     5761 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
     5821 cagaccattg cacccatcgg accagtagca aatttgcgtt atactcaact cactttggct
     5881 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
     5941 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
     6001 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
     6061 cgtccctgaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc ctggcgatag
     6121 taagaagccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     6181 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacagcct ctttccaggc
     6241 accagaagcg ggtttcgaac atcaaaaaaa aatgccgtcc gcgccagcgc ctgatggcct
     6301 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgctgaaaga
     6361 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     6421 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     6481 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     6541 agctctacag ccgcacggca tcggttttct cgaaccgggg attcagattg ccaccattga
     6601 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     6661 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     6721 cgtgctggtt gcctcgaccg ttcaggaagg ggtgatgcgt aatcacaatt aaaaaaatag
     6781 ccggaggtga aaaccgtccg gctgtttttt gcagtgcttg ttacgcgtta taggcattct
     6841 cgccgtgact gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgcaggccaa
     6901 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     6961 tggcaatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     7021 ccacgccgcc cagcgaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     7081 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     7141 tggtaacgcc ccacaagccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     7201 ccccaatgta gccacaggct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     7261 cccccagcag tgaaggctta ccacgcagtg cccattcacc gaagatccag ccaagaattg
     7321 cagccgccgt tgcgaccaca gtattcacaa aagccagtgc cgcgatttca ttcgccgtgc
     7381 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     7441 ccatcggcag gttgtgtggt ttaaacgcct ctttaccgaa gcccacgcgt tttcctatca
     7501 gatacgcgcc caccagaccg gcgattgcgg cgttaatgtg caccaccgtg ccacccgcga
     7561 aatccagcgc accgtgagaa gccagcaaac caccgcccca gaccatatgc gcaattggaa
     7621 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     7681 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     7741 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt ttcagcatca
     7801 gccagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgag taaccgtaaa
     7861 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     7921 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     7981 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     8041 cagctattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     8101 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     8161 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     8221 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     8281 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     8341 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     8401 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     8461 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     8521 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     8581 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     8641 gaaatattgc gctattcagg cgctcaatga ttcttcttca cgcacgctgg ctgccagctc
     8701 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     8761 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatag tgtcggcatc
     8821 aacaatggtc gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     8881 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     8941 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     9001 gactgagaga ttatttccct gctcgcccag cggcgtgtaa ataccgtcgc tcatgctacg
     9061 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     9121 acgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     9181 cattgccacg ccctggcgca gcgcgctgtg acttagcgaa cttaatggac gaccatcaag
     9241 gcgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     9301 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     9361 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     9421 gccgctctgt aacgggcgat catcattgcc atattgctgg cgcggtccgt ccatcagctc
     9481 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgctgcg tggtcagttc
     9541 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     9601 aatggtgccg ctggcggaga agccaaacag catcaacaag ccacagagaa tgagcgatga
     9661 aaacagactc agcagcggac gcaggagaaa accgtcgagg cgcagggttt gcatcctcgc
     9721 catatagtgt gaacgactgg cctcacccat gcgttcgcca aatcgcgcct gctggcggaa
     9781 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     9841 ataggcgcgc acacgacgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     9901 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     9961 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
    10021 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
    10081 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
    10141 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
    10201 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
    10261 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
    10321 ttctgccgct gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
    10381 cgaaccgtac gctaacagac gcttgagagt cggccacagt tggctaaaac tacgcatcga
    10441 cggcctcctc gcgaatttcc ggagcgtcgt cgagcgccgc ctccagttgt tgatagcgat
    10501 acatatcgcg ataccagcca ctttgttgcg ccagctcatc atgattgcca cgctgggcga
    10561 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgcagaa aggcgatggg
    10621 cactgatgat taccgttctt ccctgccccc actgacgcag gttatgcagg atctggtgct
    10681 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
    10741 ataacgcacg agcaatggag atacgctgtt tttgcccgcc ggaaagcatc acaccgcgct
    10801 cgcccacctc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
    10861 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
    10921 tcgccacggt gtcagaaaaa aggaatggcg tctggctaac taccgccagg cggctacgcc
    10981 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
    11041 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
    11101 agatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
    11161 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
    11221 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
    11281 acgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
    11341 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
    11401 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
    11461 tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
    11521 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
    11581 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
    11641 gcttaaagcg ttcatgcaaa gcatcgccgt tgcgcttaat catgatcgcc atcactggca
    11701 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
    11761 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
    11821 cgacgcgatc gacgtcatta gtcgcacgag ccatgaggtc acccgtgcga tgacgcaggt
    11881 aaaactcagg atgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
    11941 gttgataaga cgcaccaaac agcaatacgc gccagacgta acgcaggaga taaaccacaa
    12001 cggcaatcag caccatggtg gcgatccaca tcaggatctg cccggtagta aagtgttgtt
    12061 ctgtcacgcc atcgacaaca ataccaacca cttttggtgg aaccagttgc agcatcgcga
    12121 taatgacaag caaggcgaca gccccgagat agcgacgcca ttcccgacgg aaataccagc
    12181 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
    12241 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
    12301 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
    12361 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
    12421 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
    12481 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
    12541 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcaag gggtggttgt
    12601 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
    12661 cagcagctta cggtcaattt tatctaacat agcccttcca cagagaattt ttttctcttt
    12721 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
    12781 acaaaatgac aaattcaacg caaaaaaaag ccgggcaatt gcccggtgct ggaaggaatc
    12841 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
    12901 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
    12961 cggcatcgca ttgcccataa taaatccgct accgacgctg cctaacattt cgcgatcgtt
    13021 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagtac
    13081 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcaaggcaat ccgtggcgga
    13141 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca aacgtgtaag
    13201 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
    13261 atcgattatt tgataacgaa aaccgctata gacaaatgcc tgcaacaacg cagggatctc
    13321 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
    13381 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
    13441 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tacgcatcca gcgatagcgc
    13501 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
    13561 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat gatcaggcat
    13621 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
    13681 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
    13741 ataatattgt tccgggttta tatgcgactg ctcaaccgtc ttaaccagta tcaacgtctg
    13801 tggcaacctt ccgccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
    13861 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
    13921 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgcca
    13981 gaatcgctac gcaatgcgat gatggaaaag gcactggaaa ccggaaagca gcaagatgtg
    14041 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
    14101 ggacaatggc aaaacgatac gcccacgttg cgtattccct actatcgccc gctcgaaccg
    14161 ctacaaccgg gctttttgcc cggccgtgcc gagcagcatc tcgccggaca gatattttcc
    14221 ggcctgaccc gcttcgataa taatacccag cgcccgattg gcgatttagc gcatcactgg
    14281 gaaacctctg ctgacgggtt acgctgggac ttttatcttc gttcaaccct acactggcat
    14341 aacggcgatg cagtaaaagc ctcacactta caccagcgat tattgatgct gttacaactg
    14401 ccagcactgg atcaattatt tattagcgtg aagcgtattg aggtcaccca tccgcagtgt
    14461 ctgaccttct ttttacatcg ccctgattac tggcttgcgc accggctggc gagctattgc
    14521 agccatctgg cgcatccgca attcccactg atcggcacgg gtccttttcg cttaacacaa
    14581 ttcacagcgg agctggtgcg cctggaaagc catgattatt accatttacg tcatccgctg
    14641 cttaaagcgg ttgagtactg gataactccg ccgcttttcg aaaaagattt gggaaccagt
    14701 tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg agctgcaacg ggtcagccag
    14761 gtcagtagtg gcatcagttt aggtttttgc tatttaacgt tgcgcaaaag tccccgactc
    14821 tccctctggc aggcgcgaaa agtaatctcc attattcatc aatccggttt attacaaacg
    14881 ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat tactgccagg ctggactatt
    14941 ccgcattggc aagtaccgga tgaagtcaaa ctaccgaaaa ccttgacgct ggtttatcac
    15001 ctaccgatag aacttcatac catggcagaa cgcctacagg cgacactggc agcggaaggc
    15061 tgtgaactca caattatttt tcataacgca aaaaactggg acgacacgac cctactggca
    15121 cacgcagacc tcatgatggg cgacagatta attggcgaag caccggaata tactctggag
    15181 caatggctac gttgcgatcc actgtggcca catgttttcg acgctccagc atatgcccat
    15241 ttgcaatcga cactggacgc ggtgcaagta atgcctgatg aggaaaaccg atttaatgcc
    15301 ctgaaagcgg tttttagcca gttaatggca gacgcgacgc tgacgccgct attcaactat
    15361 cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc gactgacacc gcgcggctgg
    15421 tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat gaatagctgg tccgggttaa
    15481 tcgcaggcgt taccataacg cttttattgt tcaagcagga ttatctatga aacgtgctgt
    15541 cgttgtgttc agtggaggtc aggattccac cacctgtctg gtgcaggcat tacaacaata
    15601 tgatgaagtc cattgcgtga cgttcgatta cggtcagcgc catcgcgcag aaatcgacgt
    15661 ggcacgcgaa ctggcgctga aactgggggc acgcgcgcat aaggttcttg atgtaacgct
    15721 gctcaacgag ctggcggtca gcagcctgac gcgtgacagc attccggtgc ctgattatga
    15781 acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg cgtaatattt tgttcctgac
    15841 actggcggca atatatgcgt atcaggtaaa agcagaagcc gtaattactg gcgtctgcga
    15901 aacggatttc tccggctacc cggattgccg cgatgagttt gtgaaagcac taaaccatgc
    15961 cgtcagtttg ggcatggcga aagatattcg ttttgaaacg ccgctgatgt ggattgataa
    16021 agcggaaacc tgggcgctgg cagattatta cggcaaactg gatttagtcc gtaacgaaac
    16081 gttgacctgc tataacggca ttaaaggcga cggttgcggt cattgtgcgg cgtgtaattt
    16141 acgcgccaac ggtttgaacc attatctgac cgataaaccg acggtgatgg cagcgatgaa
    16201 gcagaaaaca gggttgaagt aattattcca agtattgccg gatgcggctt gagcatccgg
    16261 cacaacaaaa cgtttactta accatctgct ccagcttttc gcgcaattcc ccttccagag
    16321 ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt aataagcgca tccgctacta
    16381 cctgcccttc cggctccagt gtaatgacct ggcttaagat gccgctttta ccgtttaatt
    16441 gctgtaactg gctcgtaatg gttaacaagt cgcttaatac cgccgggcga cgatagttaa
    16501 tattgatatt caccaccacg aaagcgatgt tatgggccgt catccagtga aaactgtcgc
    16561 tattttccaa cccatcccag cgggcttctt cgagaaattc aaggtagcgg gcgttgttta
    16621 cgtgctggta aacgtcgaga tgatatccac gaactttgat ttgtgtttgc atagcgcaat
    16681 aaccttacgt tgtttttata aaaactgtta taagtacaga ggtcataact ggtatgacct
    16741 cttcagtctg gcaaaaaatt gccactatgc aaattaatta cagggttaat accgccagat
    16801 tacgttccac cagcgaattg cccatccccg gcacctgctt tagatcctcc accgttttaa
    16861 acggaccgta ctcttcgcga taactgacaa tcgcctgcgc tttcttcagg ccaacgccat
    16921 tcatcgcgcg ggctagctct tccgcgctgg cattattaat gctgacccgg gtgccttctt
    16981 cgtcactggc tttcgccggt actgctgctt tactttgtgc tgcaggagct tccgctttgg
    17041 tttctaccgc cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg
    17101 cacaggccag ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc
    17161 cttgtttgct aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg
    17221 ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt tacagctgca aagcattttt
    17281 ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct
    17341 ctttacgcag gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac
    17401 cctgcaccat cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg
    17461 ccagcagaac cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg
    17521 ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg
    17581 ttttcggctc tccaaatttc agaccggcag cctgcatagc ttccgcacct ttgccggctt
    17641 tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct
    17701 gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt
    17761 gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga
    17821 tgcccggcgc gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct
    17881 tgaagttcaa ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa
    17941 cgccggcagc ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca
    18001 ctttctgctg cagcgcgtag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa
    18061 tgtcgtcacg tactttgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta
    18121 caatcaggaa accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac
    18181 cagcattttt cagttcatcc gggatagtgg catcttctaa ccaacccata tcgccgccgt
    18241 tacgagcaga gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt
    18301 tattcagctc atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt
    18361 agcgggtacg ctgcggctgg gtgaattgat cctgatgctg gtcgtagtag ctctggatat
    18421 ccgcatcgct aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga
    18481 attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt
    18541 cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat agtcgcttca cgtaccacgc
    18601 gttgttgagc gactagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac
    18661 cggcaacgcc gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact
    18721 gatcggcggt catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc
    18781 cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcgctgatac
    18841 ccagtttcag ctcacgtgcg tactgatcca gcagcgcctc gtcgatcaga cgattcagca
    18901 cctgttgacg cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc
    18961 ccagctgttg ctgcatgcga ttacgctcgc tgttgaaggc attctcgaac tgcccacggc
    19021 tgatttcctg gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac
    19081 tcacgccggt caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac
    19141 tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact
    19201 gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca
    19261 attgtcagcc cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag
    19321 atgcgccctt gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt
    19381 cagtgcttta cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc
    19441 ggtctgcggg ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc
    19501 taccagtgct acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc
    19561 taacgcacgg ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa
    19621 ttgagattta ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa
    19681 ggtacgaacg cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag
    19741 ggcaagcccg atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta
    19801 gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa
    19861 cctgcatgcc agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca
    19921 caggatgaat gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat
    19981 tttcgaacgg aattaacact gttttaatcc caccgcgatg cgctgccagt agtttttctt
    20041 tcaaaccacc gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat
    20101 cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccag
    20161 cactcggacc atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcgcgttttt
    20221 cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg
    20281 cctgaatgga ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttcc
    20341 ccggaacgca cgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac
    20401 cggttacctg gccgacacgg ttttcgttat cagcgcgtcc atagtcgaaa cgctgaacac
    20461 cgagatagtc atgcaagtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca
    20521 gtaactgctt aaccgcttta cggcacagtt tggagatttc acgctccaga ccacgcacgc
    20581 ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct
    20641 cacctttttt cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt
    20701 tcagtttttc atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg
    20761 gtgccggaat gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat
    20821 ccacttccag gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca
    20881 gtgcagaggc cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga
    20941 acagcgggtt tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac
    21001 cgatgtaagt acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca
    21061 tacggacata tttacgcccg gtggctttgg cgatggactg acccagggag gttttaccta
    21121 ctcccggcgg ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa
    21181 ccgcaagata ctcaaggatt cgatctttca cgcgctccag accataatgg tcggtatcaa
    21241 ggatttcctg cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct
    21301 gtaccatcca gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca
    21361 ttttcagctt ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg
    21421 cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt
    21481 ctttctgaat agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct
    21541 gctttttaac gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca
    21601 tcattgccat cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt
    21661 tgtcagccag tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt
    21721 cgatgctatt cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc
    21781 cttcgaactg gctgattgca gtacgcacca gcacttcctg ttctcgctca tcaatggtcg
    21841 gcgactccag atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa
    21901 tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca
    21961 tctgcaatat agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg
    22021 ttgaagcttc tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca
    22081 gacaacggat agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca
    22141 ccacatcgcg cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag
    22201 agctctctct tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca
    22261 tctattaaca tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag
    22321 aggaaaaatt aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg
    22381 gttaattatt caccagatgc ctgttgcgct tccggcttgc cataaatcag caacggtttg
    22441 ctttgaccat caattaccga ctcgtcgata accacttttt cgacatcttc catggacggc
    22501 agatcgtaca tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg
    22561 gttttacgcg ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga
    22621 tccacgcctt ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct
    22681 ttgaggatct gaatcagagc ttcttcgctc agttcattca acgttgcgac aaccggcaga
    22741 cgaccaataa actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc
    22801 agcagctcgc cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg
    22861 ccggagccgg tttctacacg atgggaaatc actttatcca gaccggcaaa cgcaccgcca
    22921 caaataaaca ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc
    22981 ccaccttgcg gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt
    23041 acgccttcac cggaaacgtc acgggtaata gacgggttgt ctgacttacg agaaatcttg
    23101 tcgatttcat cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt
    23161 tgcaacagct tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc
    23221 gtggttgcgt cggccatggt gaacggaacg tccagcaggc gcgccagcgt ttcagccagc
    23281 agcgttttac cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgacg
    23341 ccattgctgg tatcgccgtt gcgcagacgt ttgtagtggt tgtataccgc gaccgccagc
    23401 acttttttcg cctgttcctg gccgataacg taatcgtcca ggtggttacg aatttcatgc
    23461 ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg
    23521 atgtcgttac ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc
    23581 ttgcgcactt catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca
    23641 tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac
    23701 gacgcaaatg ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta
    23761 tagcggcaca gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac
    23821 cgtattccac cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct
    23881 gttctaatga tttacccgta tgaagcgcca taagttcatt catacgccct ttaactttca
    23941 gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt
    24001 ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca
    24061 gcaagaaagc gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga
    24121 taaactgcat ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa
    24181 tgtacagata gatatctttt tctgggtttt ctgcttccag gaacagcatc tgcgccacaa
    24241 tcaggttagc catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta
    24301 gacgagaata gatatcaaaa gagcgctcac cacgtgaggt ctgttcaatg accatcggca
    24361 ccagcgccat atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct
    24421 ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag
    24481 tccctgtgtt atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg
    24541 ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc
    24601 accacctggc ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc
    24661 ctgctggttc atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag
    24721 tacagcttca acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt
    24781 gtttttgctg tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc
    24841 gatcaggcct ttcacgcgct cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc
    24901 cagcagcagg ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag
    24961 ttccagagct tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc
    25021 gatttcgctg tcaatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc
    25081 gatcgcctga gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt
    25141 acgcacttca gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa
    25201 ttctgcagtc agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt
    25261 cgctgcttta cctttcaggt tttctgcgtg gtattcttcc gggaaggtca cgtcgatgga
    25321 gaactcttcg ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc
    25381 ctggcccatc gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac
    25441 agaaccggtg aagtcgatgg ttacgcggtc ttctgcttca acagcgccgt ctttttcttt
    25501 ccaggtcgcc tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac
    25561 ttcaacgatc ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata
    25621 aacttcaaac tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata
    25681 agtcggtgcg ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct
    25741 catcaggtca cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt
    25801 cattggcact ttgcctttgc ggaagccgtc aatacgtact ttttttgcaa cgttgaccag
    25861 ctcgctttta acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc
    25921 aaggccttga gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct
    25981 cggtcaactc ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat
    26041 aatcaatcac attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat
    26101 tatagcggca tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc
    26161 acaattcccg ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac
    26221 aaaacggccc gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac
    26281 agttgcaaat gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga
    26341 cggtgtcctg caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa
    26401 cggtagtaga gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac
    26461 gttcacccgt aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg
    26521 ggacattgtg acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc
    26581 ttaatttttc ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag
    26641 atgtcatcca tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca
    26701 aatatttagg tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat
    26761 tgaaacacag gcctcgcatc agcgtttctt ttaccagggg cgattatttc gtcaacatga
    26821 tgaatttaca tgccttacct acttcccctc gccgttggca atgttatgat ggcgggaatt
    26881 tttaccaaac acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc
    26941 cgttagttgc tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt
    27001 ccccgacgat tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta
    27061 atggtgccga tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg
    27121 ttaccctgac cgcctcccgc gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga
    27181 tgaccgcgcg tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta
    27241 gccaactgta tgctgacgtg tcccagggca acgtgcgcta caacatcgct accaaagcgg
    27301 atatcgccat catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca
    27361 gctacaacgt tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca
    27421 gtgtgctgac cgataccatc gctgatatgt ctcaggacac cagcattcac gaattcatca
    27481 agcagaacgc gcgttaattt ctgccctctg gcccggtgca agccgggcct gtagacgccc
    27541 atgtccagtc aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg
    27601 ggttttgctt ccgggctacc gctcgccctg acatccggca ccttacaggc ctggatgacg
    27661 gtcgagaata tcgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtc
    27721 tttaaattcc tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg
    27781 cgcggttggc tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt
    27841 ctcgaaccag gcacccaact ccgctggatg gcggcgctgg cggtggtgat cgctttttgc
    27901 tctgcctccc aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa
    27961 cgtggtgcgg gcgcggcaat cagcgtgctg ggttaccgtt tagggatgtt ggtttccggc
    28021 ggcctagccc tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg
    28081 gcggcgctgt tgatcccctg tattatcgcg acgttgcttg caccagaacc aaccgacacc
    28141 attcctgtgc ccaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt
    28201 cgcaataatg cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc
    28261 gccatgagcc tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta
    28321 ggcgtggtta acaaaacact gggcttactg gcgaccattg ttggcgcatt gtacggtggg
    28381 attttgatgc agcgcctgtc actgttccgg gcactgctga ttttcggcat tttacaaggt
    28441 gcgtctaacg ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc
    28501 gcagccgtct ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcgctg
    28561 ttaatgacgc tatgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt
    28621 tctgctgtag ggcgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc
    28681 tggtcgacat tctatctatt ctccgtcgcc gctgccgtac cagggcttat tttgctgctg
    28741 gtttgccgcc agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgca
    28801 tatccggcag gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc
    28861 gtgtggttac tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct
    28921 gctctgctgg aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg
    28981 gattatctgg cgctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag
    29041 taatcactcg ccgtaattat tacggctaaa taaacatcag catcgcttat taatatttgt
    29101 tcttttgtgc ggcttagcgt ttggaaatta ttggcgccat ttataaattc tatttttctt
    29161 acacgattca gctaatgagt ctttattttc tcacccccag ttgtcactcc aatgataatt
    29221 atttgttaaa taattgtttt atttcgtatt ggttatacca attgcccgcc cagactcctt
    29281 ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc aacctgatat
    29341 gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta atcatgttta
    29401 cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag atcccgtgga
    29461 attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg ttgtcattat
    29521 ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc aaaggacaga
    29581 ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg attgtcgtta
    29641 ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc aataaagatg
    29701 ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc tggacggtac
    29761 ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac gctcttgagc
    29821 ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt tccatggact
    29881 ggaaatggtt cttcatctac ccggaacagg gcattgctac cgtgaatgaa atcgctttcc
    29941 cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac tccttcttca
    30001 ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc ctgcatctga
    30061 tcgccaacga ccccggcact tatgacggta tctccgccag ctacagcggc ccgggcttct
    30121 caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgcattcgac cagtgggtcg
    30181 caaaagcgaa acagtcgccg aactccatgt ctgacatggc agcgttcgaa aaactggccg
    30241 cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac ttgtttgccg
    30301 atgtaattaa caagtttatg gctcacggta agagcatgga catgacccag ccagaaggtg
    30361 agcacagcgc acacgaaggt atggaaggca tggacatgag ccacgcggaa tccgcccatt
    30421 aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca gtcccgttcc
    30481 atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg gcgctcgttg
    30541 gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg acctccgtcg
    30601 accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg ctgcgtggtt
    30661 ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc gaagcgggct
    30721 tcctgccacc tcaccactac gatcagatct ttaccgcgca cggcgtgatt atgatcttct
    30781 tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg cagatcggcg
    30841 cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact gttgttggtg
    30901 tgattctggt aaacgtttct ctcggcgtgg gcgaatttgc gcagaccggc tggctggcct
    30961 atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac tggatatgga
    31021 gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc gttaccattc
    31081 tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc tgggcatcac
    31141 tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc gtcgcgttgt
    31201 tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt ggcaacatga
    31261 tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc ctgatcctgc
    31321 ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt ctgtttggtt
    31381 atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc gtttggctgc
    31441 accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt atcaccacaa
    31501 tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc atgtatcagg
    31561 gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc accttctcgg
    31621 tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt ctacataaca
    31681 gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc ttcggctgct
    31741 tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac gaaacctggg
    31801 gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg ccactgtacg
    31861 cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg cagttccaca
    31921 ccatgctgat gattgcagcc agcggtgcag tactgattgc gctaggtatt ctctgcctcg
    31981 ttattcagat gtacgtttct attcgtgacc gcgaccagaa ccgtgacctg actggcgacc
    32041 cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc acctccgttc tataactttg
    32101 ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag aaaggcgaag
    32161 cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc ggtgcaggta
    32221 tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat atctggtggc
    32281 tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc ttcgacgagg
    32341 acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag catttcgatg
    32401 agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc gactgcccac
    32461 gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt ttggatctac
    32521 ctgatgagcg actgcattct gttctctatc ttgtttgcta cctatgccgt tctggtgaac
    32581 ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt tctggttgaa
    32641 actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc catgtacaaa
    32701 aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg cgccggattt
    32761 atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggc tccggatcgc
    32821 agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca cgtcacttcc
    32881 ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct gaccagcact
    32941 aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt ggtttggatc
    33001 tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct accgatcaca
    33061 gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg tcgatcattc
    33121 tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc gtaattctgg
    33181 gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc ttcctgcaca
    33241 tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc gtgctaatca
    33301 tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac aacatgatga
    33361 tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga aaccaggcat
    33421 catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa agggcagcat
    33481 tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg cgtcgggttg
    33541 tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga cgaagaatcg
    33601 ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca ccttgctggg
    33661 tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct ggctgggggt
    33721 gatgggcttt gtggtttatg tcggcgttta tagcctgtac atgaaacgcc actcagtcta
    33781 cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggtt actgtgcagt
    33841 aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc tgtggcagat
    33901 gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg caaacattcc
    33961 ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc tgtatatcat
    34021 cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat ataaatatct
    34081 ggtggtcgcc gcggcggtga gcgtctggtg gttaggtatg gccctgcgcg gttataaagt
    34141 tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg ccatcactgc
    34201 cctctcggtg atgatgtccg ttgattttat ggtgccggac tcgcatacgc tgctggctgc
    34261 tgtgtggtaa cacaacctct ctattaaaaa ggtgctacgg cacctttttt cttagcatta
    34321 gaaacatatc cctctcgaaa tatttactaa aaaatccgca tgtttacccc attcgtttgc
    34381 cgctttacac tagtcgcgaa tttaaaacag aggtggtaat gaacgattat aaaatgacgc
    34441 caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt ctcgttgcgc atgctgggca
    34501 tgttcatggt tctgccggtt ctgaccacgt acggcatggc tctgcaaggt gccagcgaag
    34561 cattaatcgg tattgccatt ggtatttatg gtctgactca ggccgttttt cagattccgt
    34621 ttggcctgct ttccgaccgc attggtcgca aaccattaat tgtcggtggg ctggcagtgt
    34681 ttgccgccgg tagcgttatc gctgcgctct ctgactccat ctggggaatt attctgggtc
    34741 gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca
    34801 cgcgcgaaca aaaccgcacc aaagcaatgg cgtttatcgg cgtgagcttt ggcattacct
    34861 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
    34921 tctggatgat cgctattctg gcaacgaccg gcattgcgtt gaccatttgg gttgtgccca
    34981 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt ttcagtaaag
    35041 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
    35101 tgatgtcgac ctttgttgcc ctgcccggac aactggctga tgcggggttc ccggcggctg
    35161 aacactggaa ggtctatctg gcgacaatgc taatcgcctt tggctcggtc gtgcctttca
    35221 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc atcgggttga
    35281 tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca gttctggcaa ctggtggtcg
    35341 gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc cctcctgccc tcacttatca
    35401 gtaaagagtc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
    35461 ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc
    35521 agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
    35581 tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat cccggcgaac attgccgcaa
    35641 acgaggcgtt aaaagtgcgt ttgctggaaa ctgaaggcat caaagaagtg ttgattgcag
    35701 aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt gacgaatcgc tttgaggttg
    35761 aacaggcaat tcgccaggca taaaagcgag gcgaaattgc ctgctgcact acgcttatca
    35821 ggcctactaa gttcctgagt tacaaagctt tgtaggccga atcaggcgtt catgccgcat
    35881 ctgacatgaa caaagcgcag gttgtcgcga ttaatcgcgg aagtttttga actggaacgg
    35941 ctgacccaga tcgccaccgc gcaccatcgc catgacagcc tgcaaatcat cacgagattt
    36001 gccagtcacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
    36061 gatcatcttg acgattttct tctgggtcgc gctctcaatg ccctgtttca gtttcgcttc
    36121 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagcg aactgccttc
    36181 aatgccgcgc ttcagcagct tggcacgcaa aatatccagc aactgattga cctggaaatc
    36241 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
    36301 gttacggaag tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc
    36361 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
    36421 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
    36481 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
    36541 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
    36601 tagggcaatt atggcttaca gcactttgta aacagggtca cgaagttcag ggctggctgc
    36661 gcgtaccgca accttattgt agtgtgaatc tggttgagac tgatggttcg atatttaacg
    36721 aatcgctgac cgccaacgat cccgattttc ttgccaccag cgatctgctc ctggtgacgc
    36781 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacta
    36841 cgccaatact gttaattcac aacggcatgg gcaccatcga agaattgcaa aacattcagc
    36901 agccattact gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc
    36961 atgtggcaaa cggtatcacg catattggtc cagcacggca acaggacggc gattacagtt
    37021 atctggcgga tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
    37081 ccgagctgtg gcgcaagctg gcagtcaact gtgtgattaa tccactgact gccatctgga
    37141 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag
    37201 tcgcggcagt gatcgaacgc gaagggcatc atacttcagc ggaagatttg cgtgattacg
    37261 tgatgcaggt gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgcg
    37321 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
    37381 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
    37441 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
    37501 accacgatcg acctcttggt tcgcggcggt atcaaagtca ccactgccag cgtagccagc
    37561 gatggcaacc tggcgattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
    37621 gtcgaagtgg ctgatggcga atatgacgtg atcgtactgc ctggtggcat taaaggcgca
    37681 gagtgttttc gcgatagtac cctgctggtt gaaaccgtta aacagttcca ccgttccggg
    37741 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtcccgca cgatatcttc
    37801 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc cgaacaatgg
    37861 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
    37921 acagctatcg actttggtct gaaaattatc gacctgttgg ttgggcgtga aaaagcccat
    37981 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
    38041 atgcggtaag cgtcgcatct gaccagatgc gacgttacaa ccacaaatta cgggcgatat
    38101 accttcacat tgttaaagcc ctgctcgcgc agatagagcg cctgcagacg gctcatcacc
    38161 ccgcgctcac accacaacag ccaggtttta ctctggtcga gatcgccaaa tttggtgctc
    38221 agtttataga acggcagaga aaccacatca accccttcga ctttcagtgg cttatcttct
    38281 tgttcatcga tagaacggat atcgaggatg acgtcattcg ggccgaagcc attgacagtt
    38341 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
    38401 gcttcctcaa ccactttatc gagaatgctg aagtcgaact tctcttcttc cgcttcaatc
    38461 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca caccgcagta ttccggcatc
    38521 gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
    38581 taagagatca gcggacgcag gatcagcgta tcagagacgt tatcaatcag gcgcaggttg
    38641 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
    38701 taacgttcag ccactttaga cgcggcacgc accatcatac gtttgaggat aacgcccatc
    38761 tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg gctcgaaatt aatagcgaca
    38821 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgtacg
    38881 ccaatttcat gcgccgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga
    38941 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagtaca
    39001 tcttcctggg tgccgatcgg gaaaccacca ataccttcgt agcggccttt aatcagcagg
    39061 agacgatcgt cttccacttc caaatggaca gtcacatccg gattggtcag cttcacgcgc
    39121 gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
    39181 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg
    39241 cgatactgaa ccaacgcttt ctcgaaaata tcgtgcatgt cggtaaacgg cacatcttcg
    39301 acttcgagaa tatggtggat acccggaata cgggtcagag cgtcgcgaat agccagacgc
    39361 tggttttcat cttttgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
    39421 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
    39481 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
    39541 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
    39601 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
    39661 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
    39721 attgagagtc agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
    39781 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
    39841 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
    39901 caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc
    39961 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
    40021 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
    40081 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct tttctggttt
    40141 atgccaccgg tcatatgttc ggcgttagca caaacacgct ggacgcaccc gctgctgccg
    40201 ttgagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
    40261 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcgaac gcgattctcg
    40321 ctggcgatgc tttacaaacg ctggcgttct cgattctaag cgatgctgat atgccggaag
    40381 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
    40441 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacatgta cctctggacg
    40501 cactcgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
    40561 gtgcattaag cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag
    40621 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggtg ggagatactg
    40681 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
    40741 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgccagt
    40801 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
    40861 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
    40921 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccaa
    40981 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
    41041 gttccagtgg gcacttcgcc tccgggctgg gcacggtcga gctgaccgtg gcgctgcact
    41101 acgtctataa cactccgttt gaccagttaa tctgggatgt ggggcatcag gcttatccgc
    41161 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggtggcctgc
    41221 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcgga cattcatcaa
    41281 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
    41341 gcaccgtctg tgtgattggc gatggcgcga ttaccgcagg catggcgttt gaagcgatga
    41401 atcacgcggg cgatatccgt cctgatatgc tggtggttct caacgacaat gaaatgtcga
    41461 tttccgaaaa tgtcggtgcg ctcaacaacc atctggcaca gctgctttcc ggtaagcttt
    41521 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgttccgccc attaaagagc
    41581 tgctcaaacg taccgaagaa catattaaag gcatggtggt gcctggcaca ttgtttgaag
    41641 agctgggctt taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca
    41701 cgctgaagaa catgcgcgac ctgaaaggcc cgcagtttct gcatatcatg accaaaaaag
    41761 gtcgtggtta tgaaccggca gaaaaagacc caatcacctt ccacgctgtg cctaaatttg
    41821 atccctccag cggttgtttg ccgaaaagta gcggtggttt gccgagctat tcaaaaatct
    41881 ttggcgactg gttgtgcgaa acggcagcga aagataacaa gctgatggcg attactccgg
    41941 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgctacttcg
    42001 atgtggcaat tgccgagcaa cacgcggtga cctttgccgc cggtctggcg attggtgggt
    42061 acaaacccat tgtcgcgatt tactccactt tcctgcaacg cgcctatgat caggtgttgc
    42121 atgacgtggc gattcaaaag cttccggtcc tgttcgccat cgaccgtgcg ggcattgttg
    42181 gtgctgacgg tcaaacccat cagggcgctt ttgatctctc ttatctgcgc tgcataccgg
    42241 aaatggtcat tatgactccg agcgatgaaa acgaatgtcg cctgatgctc tataccggct
    42301 atcactataa cgatggcccg tcagcggtgc gctacccgcg tggcaacgcg gtcggcgtgg
    42361 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
    42421 aactggcgat ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc gccgaatcgc
    42481 tgaacgccac gctggtcgat atgcgttttg tgaaaccgct tgatgaaacg ttaattctgg
    42541 aaatggccgc cagtcatgaa gcactggtta ccgtagaaga aaacgccatt atgggcggtg
    42601 caggcagcgg agtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
    42661 ttggcctgcc ggacttcttt attccgcaag gaactcagga agaaatgcgc gccgaactcg
    42721 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc
    42781 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    42841 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    42901 cagatcgtgg taatcacgca tggacactgc cggaagagag cagccgtccc ataattaaac
    42961 gcgcgctgga aggcggcata aatttctttg ataccgccaa tagctattcc gatggcagca
    43021 gcgaagagat cgtcggtcgc gcactgcggg atttctcccg tcgtgaagac gtggtcgttg
    43081 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    43141 tgcgctctat cgacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
    43201 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctc aacgacgtgg
    43261 taaaagccgg gaaagcgcgt tatatcggcg cgtcatcaat gcacgcttcg cagtttgctc
    43321 aggcgctgga gctacaaaaa cagcacggct gggcgcagtt tgtcagtatg caggatcact
    43381 acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc gctgtgttat caggaaggcg
    43441 tggcggtgat tccgtggagc ccgctggcac ggggacgtct gacgcgtccg tggggagaaa
    43501 ccactgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaga
    43561 atgacgcgca aattgcggag cggttaacgg gcgtcagtga agatctgggc gcgacacgag
    43621 cacaagtcgc actggcatgg ttgttgagca aacctggcat tgctgcaccg attatcggta
    43681 catcgcggga agaacagctt gatgagctac tgaacgcggt ggatatcact ttgaagccag
    43741 aacagattgc cgaactggaa acgccgtata aaccgcatcc ggtcgtagga tttaagtaaa
    43801 tttcgctttt cgcccatttt atctgctgaa ttccccatcg tctgcgatga tggggacaac
    43861 gtacctccca tcaccttctg taaccataat ccacctctgc aagagaaatt cttcgtgatt
    43921 aaatttacag ctattcatca tcttcatcgc tttgagcgat ttttgatagt ttctcccgat
    43981 aatatcccct aaaaatgtct tatttagtca atgtaagagg attacaatca tgatgaaaac
    44041 catcaccaaa caaccgatac tgtttactga tgtaccggtt gcagatttaa gaaactcaat
    44101 gaagcaagat ttaaatcaaa atcttattga aaggctgtgg aacaaaattc gtgatttttt
    44161 tctggacagt gataaacaaa aggcatttaa atcgattcat aaatacatca atacattgtc
    44221 agttctaaat tataattcag cgttaactcc agatcccaac tttaatatag atgcaacgtc
    44281 agatttagac tcatatttaa agctggattt tgatcgcctc tcgcccaaac aaaagcagac
    44341 cacgctttgt tgtttctgga ataaaattgc atcctcattg ccagagccat acaacagtac
    44401 catcaaacat aacattattt tttataaaga cggagaaaat ctgatgatac gtggaactat
    44461 ctctattgtt aacgaggtag taaagactta ttcactccca atagaaaagg atgacaatgg
    44521 atattacgat ttcagcggtc tatatctagc acacagcaat atttccggta aagatcccaa
    44581 taaagatccc gatatagatt tcggtataga tatgggaaac tgtaactgct caaatgtcaa
    44641 cttcgaacat acttattttt atggcgtcaa gtttacaaat gcgaattgta caaatgcgaa
    44701 ttttaacaat tgtagattta agaaatgcga cctgacaaat atgaattgca ctggcgcaat
    44761 tcttgataat gcgatgatat atggtaaaga aaaagaacct gagatgcaat atccagaagc
    44821 agatcaaata atacaaagga taacttatca gaaatcagat gggaatgaaa cgaaagggat
    44881 gattctaact aactgctcct gtgttaaaac cactttcaat tgggcagatt tatcagaaag
    44941 tgattgccaa aatgtggact ttagcgaggc taatctttca aacactattc ttcctgacat
    45001 cgtcaggatg aaaggcacga aactttaccg taccgacctt tttaatccga tattaaaaac
    45061 aga
//
LOCUS       DAZGCQ010000031        44370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000031 DAZGCQ010000000
VERSION     DAZGCQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.031"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..3927
                     /gene="narG"
                     /locus_tag="ACHK07_001849"
     CDS             184..3927
                     /gene="narG"
                     /locus_tag="ACHK07_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO8102190.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            3924..5462
                     /gene="narH"
                     /locus_tag="ACHK07_001850"
     CDS             3924..5462
                     /gene="narH"
                     /locus_tag="ACHK07_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8102191.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMV
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5459..6169
                     /gene="narJ"
                     /locus_tag="ACHK07_001851"
     CDS             5459..6169
                     /gene="narJ"
                     /locus_tag="ACHK07_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8102192.1"
                     /translation="MIELVIVSRLLEYPDAALWQYQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LMKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6169..6846
                     /gene="narI"
                     /locus_tag="ACHK07_001852"
     CDS             6169..6846
                     /gene="narI"
                     /locus_tag="ACHK07_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8102193.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7030..7161)
                     /locus_tag="ACHK07_001853"
     ncRNA           complement(7030..7161)
                     /ncRNA_class="other"
                     /locus_tag="ACHK07_001853"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7208..7292)
                     /locus_tag="ACHK07_001854"
     tRNA            complement(7208..7292)
                     /locus_tag="ACHK07_001854"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7256..7258),aa:Tyr,seq:gta)
     gene            complement(7327..7458)
                     /locus_tag="ACHK07_001855"
     ncRNA           complement(7327..7458)
                     /ncRNA_class="other"
                     /locus_tag="ACHK07_001855"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7502..7586)
                     /locus_tag="ACHK07_001856"
     tRNA            complement(7502..7586)
                     /locus_tag="ACHK07_001856"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7550..7552),aa:Tyr,seq:gta)
     gene            complement(7746..8588)
                     /gene="purU"
                     /locus_tag="ACHK07_001857"
     CDS             complement(7746..8588)
                     /gene="purU"
                     /locus_tag="ACHK07_001857"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO8102194.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(8638..9096)
                     /gene="ychJ"
                     /locus_tag="ACHK07_001858"
     CDS             complement(8638..9096)
                     /gene="ychJ"
                     /locus_tag="ACHK07_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO8102195.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAVLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9209..10114
                     /gene="rssA"
                     /locus_tag="ACHK07_001859"
     CDS             9209..10114
                     /gene="rssA"
                     /locus_tag="ACHK07_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO8102196.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10206..11219
                     /gene="rssB"
                     /locus_tag="ACHK07_001860"
     CDS             10206..11219
                     /gene="rssB"
                     /locus_tag="ACHK07_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO8102197.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            11421..12329
                     /gene="galU"
                     /locus_tag="ACHK07_001861"
     CDS             11421..12329
                     /gene="galU"
                     /locus_tag="ACHK07_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO8102198.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(12473..12886)
                     /gene="hns"
                     /locus_tag="ACHK07_001862"
     CDS             complement(12473..12886)
                     /gene="hns"
                     /locus_tag="ACHK07_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO8102199.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            13490..14107
                     /gene="tdk"
                     /locus_tag="ACHK07_001863"
     CDS             13490..14107
                     /gene="tdk"
                     /locus_tag="ACHK07_001863"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO8102200.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(14409..17084)
                     /gene="adhE"
                     /locus_tag="ACHK07_001864"
                     /gene_synonym="adhC"
     CDS             complement(14409..17084)
                     /gene="adhE"
                     /locus_tag="ACHK07_001864"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO8102201.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            17477..17530
                     /gene="ychE"
                     /locus_tag="ACHK07_001865"
     CDS             17477..17530
                     /gene="ychE"
                     /locus_tag="ACHK07_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO8102202.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            17561..18208
                     /gene="ychE"
                     /locus_tag="ACHK07_001866"
     CDS             17561..18208
                     /gene="ychE"
                     /locus_tag="ACHK07_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO8102203.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSFQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(18366..18662)
                     /locus_tag="ACHK07_001867"
     CDS             complement(18366..18662)
                     /locus_tag="ACHK07_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102204.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNHLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            18946..20577
                     /gene="oppA"
                     /locus_tag="ACHK07_001868"
     CDS             18946..20577
                     /gene="oppA"
                     /locus_tag="ACHK07_001868"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO8102205.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            20663..21583
                     /gene="oppB"
                     /locus_tag="ACHK07_001869"
     CDS             20663..21583
                     /gene="oppB"
                     /locus_tag="ACHK07_001869"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO8102206.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            21598..22506
                     /gene="oppC"
                     /locus_tag="ACHK07_001870"
     CDS             21598..22506
                     /gene="oppC"
                     /locus_tag="ACHK07_001870"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO8102207.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            22518..23531
                     /gene="oppD"
                     /locus_tag="ACHK07_001871"
     CDS             22518..23531
                     /gene="oppD"
                     /locus_tag="ACHK07_001871"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO8102208.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            23528..24532
                     /gene="oppF"
                     /locus_tag="ACHK07_001872"
     CDS             23528..24532
                     /gene="oppF"
                     /locus_tag="ACHK07_001872"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO8102209.1"
                     /translation="MNAVTEGRKVLLEIDDLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(24585..24914)
                     /gene="yciU"
                     /locus_tag="ACHK07_001873"
     CDS             complement(24585..24914)
                     /gene="yciU"
                     /locus_tag="ACHK07_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO8102210.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(24949..26409)
                     /gene="cls"
                     /locus_tag="ACHK07_001874"
     CDS             complement(24949..26409)
                     /gene="cls"
                     /locus_tag="ACHK07_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO8102211.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            26552..26725
                     /gene="yciY"
                     /locus_tag="ACHK07_001875"
     CDS             26552..26725
                     /gene="yciY"
                     /locus_tag="ACHK07_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO8102212.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(26779..28032)
                     /gene="kch"
                     /locus_tag="ACHK07_001876"
     CDS             complement(26779..28032)
                     /gene="kch"
                     /locus_tag="ACHK07_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO8102213.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(28333..28629)
                     /gene="yciI"
                     /locus_tag="ACHK07_001877"
     CDS             complement(28333..28629)
                     /gene="yciI"
                     /locus_tag="ACHK07_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO8102214.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            28853..29572
                     /gene="tonB"
                     /locus_tag="ACHK07_001878"
     CDS             28853..29572
                     /gene="tonB"
                     /locus_tag="ACHK07_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO8102215.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTASARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(29612..30010)
                     /gene="yciA"
                     /locus_tag="ACHK07_001879"
     CDS             complement(29612..30010)
                     /gene="yciA"
                     /locus_tag="ACHK07_001879"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO8102216.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(30115..30654)
                     /gene="yciB"
                     /locus_tag="ACHK07_001880"
     CDS             complement(30115..30654)
                     /gene="yciB"
                     /locus_tag="ACHK07_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO8102217.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(30684..31427)
                     /gene="yciC"
                     /locus_tag="ACHK07_001881"
     CDS             complement(30684..31427)
                     /gene="yciC"
                     /locus_tag="ACHK07_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO8102218.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(31455..31544)
                     /locus_tag="ACHK07_001882"
                     /pseudo
     CDS             complement(31455..31544)
                     /locus_tag="ACHK07_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            31784..32422
                     /gene="ompW"
                     /locus_tag="ACHK07_001883"
     CDS             31784..32422
                     /gene="ompW"
                     /locus_tag="ACHK07_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO8102219.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(32482..32988)
                     /gene="yciE"
                     /locus_tag="ACHK07_001884"
     CDS             complement(32482..32988)
                     /gene="yciE"
                     /locus_tag="ACHK07_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8102220.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(33034..33534)
                     /gene="yciF"
                     /locus_tag="ACHK07_001885"
     CDS             complement(33034..33534)
                     /gene="yciF"
                     /locus_tag="ACHK07_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8102221.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(33620..33799)
                     /gene="yciG"
                     /locus_tag="ACHK07_001886"
     CDS             complement(33620..33799)
                     /gene="yciG"
                     /locus_tag="ACHK07_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8102222.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            complement(34180..34986)
                     /gene="trpA"
                     /locus_tag="ACHK07_001887"
     CDS             complement(34180..34986)
                     /gene="trpA"
                     /locus_tag="ACHK07_001887"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO8102223.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(34986..36179)
                     /gene="trpB"
                     /locus_tag="ACHK07_001888"
     CDS             complement(34986..36179)
                     /gene="trpB"
                     /locus_tag="ACHK07_001888"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO8102224.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIEIGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(36191..37552)
                     /gene="trpCF"
                     /locus_tag="ACHK07_001889"
     CDS             complement(36191..37552)
                     /gene="trpCF"
                     /locus_tag="ACHK07_001889"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO8102225.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNIEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(37553..39148)
                     /gene="trpD"
                     /locus_tag="ACHK07_001890"
     CDS             complement(37553..39148)
                     /gene="trpD"
                     /locus_tag="ACHK07_001890"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO8102226.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(39148..40710)
                     /gene="trpE"
                     /locus_tag="ACHK07_001891"
     CDS             complement(39148..40710)
                     /gene="trpE"
                     /locus_tag="ACHK07_001891"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO8102227.1"
                     /translation="MQTQKPTLELLTCEGAYHDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LSFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(40732..40825)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(40802..40846)
                     /gene="trpL"
                     /locus_tag="ACHK07_001892"
     CDS             complement(40802..40846)
                     /gene="trpL"
                     /locus_tag="ACHK07_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO8102228.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            40984..41865
                     /gene="rnm"
                     /locus_tag="ACHK07_001893"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             40984..41865
                     /gene="rnm"
                     /locus_tag="ACHK07_001893"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO8102229.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDIIHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            41862..42482
                     /gene="yciO"
                     /locus_tag="ACHK07_001894"
     CDS             41862..42482
                     /gene="yciO"
                     /locus_tag="ACHK07_001894"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO8102230.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            42510..>44370
                     /gene="yciQ"
                     /locus_tag="ACHK07_001895"
     CDS             42510..>44370
                     /gene="yciQ"
                     /locus_tag="ACHK07_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO8102231.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTLGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFTR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAG"
BASE COUNT        11124 a        10691 c        11327 g        11228 t
ORIGIN      
        1 cagtatgtta tgctgtaact gttaattatc ctaaaggggt atcttaggaa tttactttat
       61 ttttcatccc catcactctt gatcgttatc aattcccacg ctgtttcaga gcgttacctt
      121 gcccttaaac attagcaatg tcgatttatc agagggccga caggctccca caggagaaaa
      181 ccgatgagta aattcctgga ccggtttcgc tacttcaaac agaagggtga aacctttgcc
      241 gatgggcatg gccagcttct caataccaac cgtgactggg aggatggata tcgccagcgt
      301 tggcagcatg acaaaatcgt ccgctctacc cacggggtaa actgcaccgg ctcctgcagc
      361 tggaaaatct acgtcaaaaa cggtctggtc acctgggaaa cccagcagac tgactatccg
      421 cgtacccgtc cggatctgcc aaaccatgaa cctcgcggct gcccgcgcgg tgccagctac
      481 tcctggtatc tttacagtgc caaccgcctg aaatacccga tgatgcgcaa acgcctgatg
      541 aaaatgtggc gtgaagcgaa ggcgctgcat agcgatccgg ttgaggcatg ggcttctatc
      601 attgaagacg ccgataaagc gaaaagcttt aagcaggcgc gtggacgcgg tggttttgtt
      661 cgttcttcct ggcaggaggt gaacgaactg atcgccgcat ctaacgttta caccatcaaa
      721 aactacggcc cggaccgtgt tgctggtttc tcgccaattc cggcaatgtc gatggtttct
      781 tacgcatcgg gtgcacgcta tctctcgctg attggcggta cttgcttaag cttctacgac
      841 tggtactgcg acctgcctcc tgcgtctccg caaacctggg gcgagcaaac tgacgtaccg
      901 gaatctgctg actggtacaa ctccagctac atcatcgcct ggggttcaaa cgtgccgcag
      961 acgcgtaccc cggatgctca cttctttact gaagtgcgtt acaaaggcac caaaactgtt
     1021 gccgtcacac cagactacgc tgaaatcgcc aaactgtgcg atctgtggct ggcaccgaaa
     1081 cagggcaccg atgcggcaat ggcgctggcg atgggccacg taatgctgcg tgaattccac
     1141 ctcgacaacc caagccagta tttcaccgac tatgtgcgtc gctacaccga catgccgatg
     1201 ctggtgatgc tggaagaacg cgacggttat tacgctgcag gtcgtatgct gcgcgctgct
     1261 gatctggttg atgcgctggg ccaggaaaac aatccggaat ggaaaactgt cgcctttaat
     1321 accaatggcg aaatggttgc gccgaacggt tctattggct tccgctgggg cgagaagggc
     1381 aaatggaatc ttgagcagcg cgacggcaaa actggcgaag aaaccgagct gcaactgagc
     1441 ctgctgggta gtcaggatga gatcgctgag gtaggcttcc cgtactttgg tggcgacggc
     1501 acggaacact tcaacaaagt ggaactggaa aacgtgctgc tgcacaaact gccggtgaaa
     1561 cgcctgcaac tggctgatgg cagcaccgcc ctggtgacca ccgtttatga tttgacgctg
     1621 gcaaactacg gtctggaacg tggcctgaac gacgttaact gtgcaaccag ctatgacgat
     1681 gtgaaagctt ataccccggc ctgggccgag cagattaccg gcgtttctcg cagccagatt
     1741 attcgcatcg cccgtgaatt tgccgataac gctgataaaa cgcacggtcg ttcgatgatt
     1801 atcgtcggtg cggggctgaa ccactggtat cacctcgata tgaactatcg tggtctgatc
     1861 aacatgctga ttttctgcgg ctgtgtcggt cagagcgggg gcggctgggc gcactatgta
     1921 ggtcaggaaa aactgcgtcc gcaaaccggc tggcagccgc tggcgtttgc ccttgactgg
     1981 cagcgtccgg cgcgtcacat gaacagcact tcttatttct ataaccactc cagccagtgg
     2041 cgttatgaaa ccgtcacggc ggaagagttg ctgtcaccga tggcggataa atcccgttat
     2101 accggacacc tgatcgactt taacgtccgt gcggaacgca tgggctggct gccgtctgca
     2161 ccgcagttag gcactaaccc gctgactatc gctggcgaag cggaaaaagc cgggatgaat
     2221 ccggtggact atacggtgaa atccctgaaa gagggttcca tccgttttgc ggcagaacaa
     2281 ccagaaaacg gtaaaaacca cccgcgcaac ctgttcatct ggcgttctaa cctgctcggt
     2341 tcttccggta aaggtcatga gtttatgctc aagtacctgc tggggacgga gcacggtatc
     2401 cagggtaaag atctggggca gcagggcggc gtgaagccgg aagaagtgga ctggcaggac
     2461 aatggtctgg aaggcaagct ggatctggtg gttacgctgg acttccgtct gtcgagcacc
     2521 tgtctctatt ccgacatcat tttgccgacg gcgacctggt acgaaaaaga cgacatgaat
     2581 acttcggata tgcatccgtt tattcacccg ctgtctgcgg cggtcgatcc ggcctgggaa
     2641 gcgaaaagcg actgggaaat ctacaaagcc atcgcgaaga aattctccga agtgtgcgtc
     2701 ggccatctgg gtaaagaaac cgacatcgtc acgctgccta tccagcacga ctctgccgct
     2761 gaactggcgc agccgctgga tgtgaaagac tggaaaaaag gcgaatgcga cctgatccca
     2821 ggcaaaactg caccgcacat tatggtcgta gagcgcgatt atccggcaac ttacgaacgc
     2881 tttacctcta tcggcccgct gatggagaaa atcggtaacg gcggtaaagg gattgcctgg
     2941 aacacccaga gcgagatgga tctgctgcgt aagctcaact acaccaaagc ggaaggtccg
     3001 gcgaaaggcc agccgatgct gaacaccgca attgatgcgg cagagatgat cctgacgctg
     3061 gcaccggaaa ccaacggtca ggtagccgtg aaagcctggg ctgccctgag cgagtttacc
     3121 ggtcgtgacc atacgcatct ggcgctgaat aaagaagacg agaagatccg cttccgcgat
     3181 attcaggcac agccgcgcaa aattatctcc agcccgacct ggtctggtct ggaagatgaa
     3241 cacgtttctt acaacgccgg ttacaccaac gttcacgagc tgatcccatg gcgtacgctc
     3301 tctggtcgtc agcaactgta tcaggatcac cagtggatgc gtgatttcgg tgaaagcctg
     3361 ctggtttatc gtccgccgat cgacacccgt tcggtgaaag aagtgatagg ccagaaatcc
     3421 aacggcaacc cggaaaaagc gctcaacttc ctgacgccgc accagaagtg gggtatccac
     3481 tccacctaca gcgacaacct gctgatgctg actttaggtc gcggtggtcc ggtggtctgg
     3541 ttgagtgaag ccgatgccaa agatctgggt atcgccgata acgactggat tgaagtattc
     3601 aacagcaacg gtgctctgac tgcccgtgcg gttgtcagcc agcgtgttcc ggcagggatg
     3661 accatgatgt accacgcgca ggaacgtatc gttaacctgc ctggttcgga aattacccaa
     3721 cagcgtggtg gtatccataa ctcggtcacc cgtatcacgc cgaaaccgac gcatatgatc
     3781 ggcggctatg cccatctggc atacggcttt aactactacg gcaccgtagg ttctaaccgc
     3841 gatgagtttg ttgtagtgcg taagatgaag aacattgact ggttagatgg cgaaggcaat
     3901 gaccaggtac aggagagcgt aaaatgaaaa ttcgttcaca agtcggcatg gtgctgaatc
     3961 tcgataagtg catcggctgc cacacctgtt cagttacctg taaaaacgtc tggaccagcc
     4021 gtgaaggcgt ggaatacgcg tggttcaaca acgtggaaac caagccgggc cagggcttcc
     4081 cgactgactg ggaaaaccag gaaaaataca aaggcggctg gatccgtaaa atcaacggca
     4141 aactgcagcc gcgcatgggt aaccgtgcca tgctgctggg taaaatcttc gctaacccgc
     4201 atctgccggg gatcgacgat tattacgagc cgttcgattt tgactatcag aacctgcata
     4261 ccgcgccgga aggcagcaaa tcgcagccga ttgcccgtcc gcgttcgctg attaccgggg
     4321 aacggatggc gaaaatcgaa aaagggccga actgggaaga tgacctgggc ggtgagtttg
     4381 acaaactggc gaaagacaag aacttcgaca acatccagaa ggcgatgtat agccagttcg
     4441 aaaacacctt catgatgtat ttgccgcgcc tgtgcgaaca ctgcctgaac ccggcatgtg
     4501 tggcgacctg cccgagcggt gcgatttaca agcgtgaaga agatggcatc gtcctgatcg
     4561 accaggataa atgccgtggc tggcgtatgt gcatcaccgg atgcccatac aaaaaaatct
     4621 acttcaactg gaagagcggt aagtctgaga agtgcatctt ctgctatccg cgtattgaag
     4681 cgggtcagcc gaccgtgtgc tcagaaacct gtgtcggtcg tatccgttat cttggcgtgc
     4741 tgctgtacga tgccgacgcg attgaacgtg cagccagcac cgagaacgag aaagatctct
     4801 accagcgtca gctggaggtg ttcctcgatc cgaacgatcc gaaagtcatc gagcaggcga
     4861 ttaaagacgg tattccgctg agcgttattg aagctgcaca gcagtcgccg gtttataaaa
     4921 tggtaatgga atggaaactg gcgctgccgc tgcatccaga atatcgcaca ctgccgatgg
     4981 tctggtacgt gccgcctctg tctccgattc agtctgcagc agacgcgggt gagctgggta
     5041 gcaacggcat tctgccagac gtcgaaagcc tacgtattcc ggtacagtat ctggcgaatc
     5101 tgctgaccgc cggtgatacc aaaccggtac tgcgcgcgct gaaacgtatg ctggcgatgc
     5161 gtcattacaa acgtgctgaa accgttgacg ggaaagtgga tacccgtgcg ctggaagagg
     5221 tcggtctgac cgaagcccag gcacaggaga tgtaccgtta tctggcgatt gctaactacg
     5281 aagatcgctt tgtggtgccg agtagtcatc gtgaactggc acgggaagcc ttcccggaga
     5341 aaaatggctg cggctttacc tttggtgatg gctgccacgg ttcagatacc aaattcaatc
     5401 tgttcaacag ccgtcgtatc gatgccatcg atgtgaccag caaaacggag ccgcacccat
     5461 gatcgaactc gtgattgtat cgcgtctcct tgaatatccg gatgctgcct tatggcagta
     5521 tcaacaagag atgtttgagg cgattgccgc gtcgaaaaat ctgccaaaag aggatgccca
     5581 tgcgctgggc attttcctgc gcgatttaac gacgatggat ccgctcgatg cccaggcgca
     5641 gtacagcgaa ctgttcgacc gtggccgcgc cacgtcactg ttgctgtttg aacatgtgca
     5701 cggcgaatcc cgcgaccgcg gccaggcgat ggtggacctg ctggcgcagt acgagcagca
     5761 cggcttgcag ttaaacagcc gcgaattgcc ggaccatctg ccgctgtatc tggagtacct
     5821 ggcgcagcta ccgcaaagcg aagccgtgga aggtttgaaa gatatcgcgc cgattctggc
     5881 attgctgagc gcgcgtctgc aacagcgtga aagccgttat gccgtgctgt ttgatctgct
     5941 gatgaaactg gcgaataccg ctatcgacag cgacaaagtg gcggaaaaaa ttgccgacga
     6001 agcgcgcgac gatacgccgc aggcgctgga tgctgtctgg gaagaagagc aggttaaatt
     6061 ctttgctgac aaaggctgcg gtgattcagc aatcactgct catcagcgtc gctttgccgg
     6121 tgccgtcgcg ccgcaatatc tgaatatcac caccggagga cagcactaat gcaattcctg
     6181 aatatgttct tctttgatat ctacccgtac atagccgggg cggtcttcct gattggtagc
     6241 tggctgcgtt atgactacgg gcagtacacc tggcgcgcgg cgtccagcca gatgctggat
     6301 cgcaaaggga tgaacctggc gtcgaacctg ttccatatcg gtattctggg gatttttgtc
     6361 ggtcacttct tcggtatgtt gacgccgcac tggatgtatg aagcctggct gccgattgaa
     6421 gtgaaacaga aaatggcgat gtttgctggt ggtgccagcg gcgtgctgtg tctgattggc
     6481 ggcgtgctgt tgcttaagcg tcgtctgttc agcccacgcg tgcgtgcaac cactaccgga
     6541 gcggatatcc tgatcctgtc gctgctcgtt attcagtgcg cgctgggcct gttgaccatt
     6601 ccgttctccg cccaacatat ggacggtagc gagatgatga aactggttgg ctgggcgcag
     6661 tcggtggtga ccttccacgg tggcgcttct caacacctcg atggtgtggc gtttatcttc
     6721 cgtctacacc tggtactggg gatgacgtta ttcctgctgt tcccgttctc gcgtctggta
     6781 cacatctgga gcgtaccggt ggagtatctg acacgtaagt accagctggt gcgcgctcgt
     6841 cactaagcga attttagttc acatagaccc tgcttcggcg gggttttttt atgggcacgg
     6901 tgcggggtga gttgtcggat gcgcttcgct tatccgacct acaggggagg atattgtagg
     6961 tccggtaaac gcggtgccat cgggcagaat tagctcggag taacaggttt tgagtatttc
     7021 aggaagaaat ggtggtgggg gaaggattac tcagcgctgc gcgcttcgcc cttcgggtag
     7081 ttgcctgcgg caacgctttc tcattggcgc tcgagtcgaa ccttggtcga agcttctcat
     7141 ccttccccgc atgggcagaa tatttaattg cggattcgtt gggaagttca gggacttttg
     7201 aaagtgatgg tggtggggga aggattcgaa ccttcgaagt cgatgacggc agatttacag
     7261 tctgctccct ttggccgctc gggaacccca ccagaggtaa ttcaaatttt gaggtaatgc
     7321 ttgaggtgct ggtgggggaa ggattattcg tcgcttcgct cctcaccctt cgggccgttg
     7381 cctgtggcaa tgttctctcg ctttcgctcg agtcgaacct tagtcgaagg ttctcaccct
     7441 tccccgatga gtgcaaactt tcacaatctc accgaagtta ccacatcgct gtggtgaatc
     7501 atggtggtgg gggaaggatt cgaaccttcg aagtcgatga cggcagattt acagtctgct
     7561 ccctttggcc gctcgggaac cccaccacgg ggtaatgctt tttactggcc tgctccctta
     7621 tcgggaagcg gggcgcatca tatcaaatga cgcgccgctg taaagtgtta cgttgagaaa
     7681 aatgaactgg ttgcgtaatt tttatccgta acggattaaa ggtaaccagt tatttttgct
     7741 ggcgattaaa gaataatcgt ccgattaccg taaacaaaga cgcgctgcgc cagtactttg
     7801 taaagcgcac gacttaagac gtttttctcg acgtcacgac ctgcgcgcat catatcttca
     7861 gctgtgtagg tatgatcgac atgaataacg tcctgcatga tgattgggcc ttcgtccaga
     7921 ttgtcattca catagtgagc ggttgcacca ataatcttca cgccacgttc ataggcctgg
     7981 tgataaggac gtgcgccgat aaacgctggc aggaaggaat ggtgaatatt gatgatctta
     8041 ttcgggaagc gtgacacaaa ttccggtgtt aatacccgca tatacttcgc cagcaccacg
     8101 tagtcaggtt gataagcatc aatggcatcc gccatctttt gatcgtgctc gttgcggctt
     8161 aacccttcat ggcttaccag ctcaaacggt atatcaaaac gctcaaccag agaacgtaaa
     8221 gtatcgtggt taccaataac tgccgcgatt tcgacatcca ggccgccgta attggctttc
     8281 atcaacaaat cgccaaggca atgcgcttct ttagtgacca gaatcactat ccggcgacga
     8341 ccggcaggat tcagctcacg cacggagcct tctggcaatg cgctatcgag atccgccagc
     8401 agggtggaat cattaaaaat cccttccagt tccgtgcgca taaaaaagcg cccggtacgg
     8461 tgatcaacaa attcattgtt ctgtacgata tttaactcgt gcttgtagca aatattggta
     8521 atacgtgcga tcagaccttt ttggtccgga caaatagtac gcagaacttt acgttggagt
     8581 gaatgcattg ctggaaaaac cttgttgaga gtgtttgcta aaccgtaccg tcaaccatta
     8641 ttggccgcag cactttttaa attttttacc tgaaccacaa gggcagggat cgttgcggcc
     8701 aaactgcgga cgtgtaccgt caatatagta ccattgaccg ttttctttca aaaatcgcga
     8761 acgttcaatt attgcacctg ttttgccacc ttcggtaaat cgagcaacaa aactgacaaa
     8821 tccgatattg tcgacgtcct gccaacaatg ttcaaatacg gtcaatccca accattcagt
     8881 atgcgcaaat ccggcaatca attcggcacg taacaccgct gccccacaag atggatgcca
     8941 ggtctttatt aaataatctg cgtcttgcat cacaaaagcg cagtaacgcg aacgcatgag
     9001 atgttctgga tcaggtgcaa ccttttcacc agacacataa gggtggcaac ataggctata
     9061 ctcgacagca ctaccacagg gacaaagctg agacacaaat aatctccctg gaaacaataa
     9121 cggcgtatta accgcctgag tagcactatg ttaaccgagc agtagcgatg tggctacgat
     9181 tgcattccag gggaatcttg cgggaataat gagaaagata aaaatagggc tggcgctggg
     9241 atctggcgcg gcgagaggtt ggtcgcatat tggcgttatt aatgcgctaa aaaaagtggg
     9301 tattgaaatt gatatcgttg caggatgttc tattggttcg ctggtgggcg cggcctatgc
     9361 atgcgatcga ttatctgcgc tggaagattg ggtaacctct ttcagttatt gggatgtttt
     9421 acgcctgatg gatctctcct ggcagcgcgg tgggttactg cgcggcgagc gtgtcttcaa
     9481 tcaataccgc gaaataatgc cggaaacaga gatcgaaaat tgttcccgtc gcttcgcggc
     9541 tgttgccacc aatttaagta ctgggcgtga attatggttt actgaaggcg atctccatct
     9601 tgctattcgc gcatcatgca gtattccagg actaatggca cccgttgcgc ataacggcta
     9661 ctggctggtt gatggggcag tcgttaaccc aattcctatt tcgctcacgc gtgcattggg
     9721 tgctgatatt gtgatagcgg tcgacctgca gcacgatgct catttgatgc aacaagattt
     9781 gctctccttt aatgtcagtg aagaaaatag cgagaatggt gattctctgc cgtggcatgc
     9841 gcgtctgaaa gaaaggttag gcagcataac gacacgtcgg gcggtgacag caccaacggc
     9901 aacagagatt atgaccactt ctatccaggt gctggagaac cgccttaaaa ggaaccgcat
     9961 ggcaggtgat ccgcccgata ttctgattca acctgtttgc ccgcaaatat ctacgcttga
    10021 tttccatcgc gcgcacgctg ccattgcggc cgggcagctg gcagtggaaa agaaaatgga
    10081 cgaacttttg ccgttggtac gcaccaacat ttgaccagaa tttttatcta cacttaagtt
    10141 aattctgaca ggcgcaggtg gcaatagcat gccactattg agtaaagcca gtcaggggag
    10201 agaacatgac gcagccattg gtcggaaaac agattctcat tgttgaagat gagcaggtat
    10261 ttcgctcgct tctggattcg tggttttcct cattgggagc gacaacggta ctggcggctg
    10321 atggggtgga tgcccttgag ttgctgggag gtttcactcc agacctgatg atatgtgata
    10381 tcgcgatgcc acgaatgaac gggcttaaac tgctggagca tatacgtaac agaggcgacc
    10441 agaccccagt tctggtgata tctgccactg aaaatatggc agatattgcc aaagcgttac
    10501 gtctgggcgt tgaagatgtt ttgctgaaac cagttaaaga tctgaatcgc ttgcgcgaga
    10561 tggtttttgc ctgtctctat cccagcatgt ttaattcgcg cgttgaggaa gaggaaaggc
    10621 tttttcgcga ctgggatgca atggttgata accctgccgc agcggcgaaa ttattacagg
    10681 aactacaacc gccggttcag caggtgattt cccattgccg ggttaattac cgtcaattgg
    10741 ttgccgcgga caaacccggc ctggtgcttg atattgccgc actttcggaa aacgatctgg
    10801 cattttattg ccttgatgta acccgagctg gacataatgg cgtacttgct gccttgttat
    10861 tacgcgcatt gtttaacgga ttattacagg aacagcttgc acaccaaaat caacggttgc
    10921 cagagttggg cgcgttattg aagcaggtaa accatttact ccgtcaggcc aatctgccgg
    10981 ggcagtttcc gctattagtt ggctattatc atcgcgaact gaaaaatctc attctggttt
    11041 ctgcgggtct gaatgcgacg ttaaataccg gcgaacacca ggtgcaaatc agtaatggtg
    11101 ttccgttagg cactttaggt aacgcttatt tgaatcaatt gagccagcga tgcgatgcct
    11161 ggcaatgcca aatatgggga accggtggtc gactgcgctt gatgttgtct gcagaatgag
    11221 caaacgatga cgcgggctaa atttgcatta ccagctaatg tcggctggtg gtactatcgt
    11281 cgccattcgt ataagtaatt gtcttaatta tgctaactcg catccttttc agaacttagc
    11341 ccctccgggg tgctgatata ctgggatgcg atacagaaat atgaacacgt tcaaaacacg
    11401 aacagtccag gagaatttaa atggctgcca ttaatacgaa agtcaaaaaa gccgttatcc
    11461 ccgttgcggg attaggaacc aggatgttgc cggcgacgaa agccatcccg aaagagatgc
    11521 tgccacttgt cgataagcca ttaattcaat acgtcgtgaa tgaatgtatt gcggctggca
    11581 ttactgaaat tgtgctggtt acacactcat ctaaaaactc tattgaaaac cactttgata
    11641 ccagttttga actggaagca atgctggaaa aacgtgtaaa acgtcaactg cttgatgaag
    11701 tgcagtctat ttgtccgccg cacgtaacta ttatgcaagt tcgtcagggg ctggcgaaag
    11761 gcctgggaca cgcggtattg tgtgctcacc cggtagtggg tgatgaacct gtggctgtta
    11821 ttttgcctga cgttattctg gatgaatatg aatccgattt gtcacaggat aacctggcag
    11881 agatgatccg ccgcttcgat gaaacgggtc atagccagat catggttgaa ccggttgctg
    11941 atgtgaccgc atatggcgtt gtggattgca aaggcgttga attagcgccg ggtgaaagcg
    12001 taccgatggt tggcgtggtt gaaaaaccaa aagcggatgt tgcgccgtct aatctcgcta
    12061 ttgtgggtcg ttacgtactt agtgcggata tttggccgtt gctggcaaaa acccctccgg
    12121 gagctggtga tgaaattcag ctcaccgacg caattgatat gctaatcgaa aaagaaacgg
    12181 ttgaagccta tcatatgaaa gggaagagcc atgactgcgg taataaatta ggttacatgc
    12241 aggccttcgt tgaatacggt attcgtcata acacccttgg cacggaattt aaagcctggc
    12301 ttgaagaaga gatgggcatt aagaagtaac atccgtatcg gtgttatcca cgaaacggcg
    12361 ttgagcaatc gacgccgttt ttttatagct tattcttatt aaattgtctt aaaccggaca
    12421 ataaaaaatc ccgccgatgg cgggatttta agcaagtgca atctacaaaa gattattgct
    12481 tgatcaggaa atcgtcgagg gatttacctt gctcatccat tgcttttttg attacagctg
    12541 gagtacggcc ctggccagtc caggttttag tttcgccgtt ttcgtcaacg tagctatatt
    12601 ttgccggacg ctgagcacgt ttagctttgg tgccagattt aacggcggca aggctattca
    12661 gcagctcgtt cgggtcaata ccgtcagcga tcagcatttc gcgatattgc tgcagtttac
    12721 gagtgcgctc ttcaacttca gcagcagccg cgctttcttc ttcgcgacgt tcgttaacaa
    12781 caacttctaa tttttccagc atttcttcca gcgtttcaag tgtacattct cttgcctgcg
    12841 cacgaagagt acggatgttg ttcagaattt taagtgcttc gctcattgta gtaatctcaa
    12901 acttatattg gggtggtttg ttgaggtaat aatagagcct taaattcagt tgtgcaatag
    12961 ccaggaatgt aagtaattca aaattgttct ttattttgtg ccgccaataa atatcttttc
    13021 ataaaattag ccagaaaaga cgcggcatat agccctattt gtaccgatga tttcgcagca
    13081 cgtgaggtta aaacttcctg attcatgtca cattttatgg ggagattatc gtaagctgac
    13141 gacctttcag tcttctgtat tagtcgtgtt tacgagaatt ccctattaag cgaatgatga
    13201 aaagtagaac agtcgcaata agagcaggga cttagtattg cactatctcc tggaggtcaa
    13261 cagagggcta ttacttgcgc aacaggttaa agattgtgaa tagttaccag cagtcattta
    13321 cccgcttata acaagcgagg cagttgtaat gatagctcag aaggattatg caaggcttcg
    13381 taagggagaa cgcatatacc acttctgtgc atactgttga gctgaaaaac tgacgaatta
    13441 tgataaactc cagccaactt tatttcatat cattgagggc ctgtggctga tggcacagct
    13501 atatttctac tattccgcaa tgaatgcggg taagtctaca gcattgttgc aatcttcata
    13561 caattaccag gaacgcggca tgcgcactgt cgtatatacg gcagaaattg atgatcgctt
    13621 tggtgccggg aaagtcagtt cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca
    13681 aaattcatca ttatttgatg agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt
    13741 actggttgat gaatgccagt ttttaaccag acaacaagta tatgaattat cggaggttgt
    13801 cgatcaactc gatatacccg tactttgtta tggtttacgt accgattttc gaggtgaatt
    13861 atttattggc agccaatact tactggcatg gtccgacaaa ctggttgaat taaaaaccat
    13921 ctgtttttgt ggccgtaagg caagcatggt gctgcgtctt gatcaagcag gcagacctta
    13981 taacgaaggt gagcaggtgg ttattggtgg taatgaacga tacgtttctg tatgccgtaa
    14041 acactataaa gaggcgttac aagtcggctc attaacggct attcaggaaa ggcatcgcca
    14101 cgattaataa gaatttcttt actgacaggg tgagcagggc acttttatcc tgtcagttcg
    14161 ttttacgcac ttcttccggg ctatacaccc ttctcggcag ttttttaacg ccgctatacg
    14221 cctcacaggg ctcttaagca ccgacgttga cttgtgatct gtaaagtaca atatcgctgt
    14281 gtttaggcgt tatacatcgt cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa
    14341 agttgctttg ggtaaacaaa aaaacggccc cagaaggggc cgtttatgtt gccagacagc
    14401 gctactgatt aagcggattt tttcgctttt ttctcagctt tagccggagc ggcttctttt
    14461 ttcgctgcag tttcaccttc tacataatca cgaccgtagt aggtatccag cagaatctgt
    14521 ttcagctcgg agatcagcgg gtaacgcggg ttagcgccgg tgcactggtc atcgaatgca
    14581 tcttcagaca gtttatccac gttcgccagg aagtctgctt cctgaacgcc agcttcacgg
    14641 atagatttcg gaatacccag ttcagccttc agcgtttcca gccatgccag cagtttctcg
    14701 atcttagcag cagtacggtc gcccggtgcg ctcagaccca ggtggtcggc aatttcagca
    14761 taacgacggc gagcctgcgg acggtcatac tggctgaatg cagtctgctt ggtcgggttg
    14821 tcgttcgcat tgtagcgaat aacgttacaa atcagcaggg cgtttgccag accgtgcgga
    14881 atatggaact gggaacccag tttgtgcgcc attgagtgac atacacccag gaaggcgttc
    14941 gcaaacgcga tacccgcgat agtcgctgca ctgtgaacac gttcacgcgc taccggattt
    15001 ttagaccctt cgtggtagga cgctggcaga tattctttca gcagtttcag tgcctgcaga
    15061 gcctgaccat cagagaactc agatgccagt acagaaacat aagcttccat ggcgtgagtt
    15121 actgcgtcca gaccaccgaa agcacacagg gacttcggca tgtccataac caggttggcg
    15181 tcgacaatcg ccatatccgg ggtcagcgca tagtctgcca gcggatattt ctgaccagta
    15241 gcgtcgtcag ttacaaccgc aaacggagtg acttcagaac ctgtaccaga agtggtggtg
    15301 acagcgatca ttttcgcttt cacgcccatt ttcgggaact tgtagatacg tttacggata
    15361 tccataaagc gcagcgccag ctcttcgaag tgagtttccg gatgttcgta cataacccac
    15421 atgatcttcg cggcgtccat cggggaacca ccacccagcg cgataatcac gtctggtttg
    15481 aaggagtttg ccagttctgc acctttacga acgatgctca gggtcgggtc agcttctact
    15541 tcgaagaaga cttcagtttc aacgcctgct gctttcagta cggaagtgat ctgatcagca
    15601 taaccattgt taaacaggaa gcggtcagtc acgatgagcg cacgtttgtg gccatcagta
    15661 atcacttcat ccagcgcgat tggcagggag ccacggcgga agtagataga tttcggaagt
    15721 ttgtgccaca acatgttttc agctcgctta gcaacggttt tcttgttgat caggtgtttc
    15781 ggaccaacgt tttcagagat ggagttacca ccccaggaac cacaacccag agtcagggaa
    15841 ggtgcgagtt tgaagttata caggtcaccg ataccaccct gagaagccgg ggtgttaatc
    15901 aggatacgag ccgttttcat tttctgaccg aagtaagaaa cgcgagccgg ttggttatcc
    15961 tggtcagtgt acaggcaaga ggtatgaccg ataccaccca tagcaaccag tttctctgct
    16021 ttttctaccg cgtcttcgaa atctttagcg cggtacattg ccagagtcgg ggacagtttt
    16081 tcatgtgcga acggttcgct ttcatcaaca acggtcactt caccgatcag aatcttggtg
    16141 ttttctggta cagagaagcc tgccagttca gcaattttat aggctggctg accaacgata
    16201 gccgcgttca gcgcaccgtt tttcaggata acgtcctgaa cagctttcag ctctttaccc
    16261 tgcaacagat agccgccgtg ggttgcgaaa cgttcacgta ctgcgtcata aacagagtca
    16321 acaacaacaa cagactgttc agaagcacag attacgccgt tgtcgaaggt tttggacatc
    16381 agtacagatg caactgcacg tttgatatca gcagtttcat caataacaac tggagtgtta
    16441 cccgcgccta cgccgatagc tggtttaccg gagctgtatg cggctttaac catgcccgga
    16501 ccaccagtcg cgaggatcag gttgatgtct gggtggtgca tcagtgcgtt agacagttca
    16561 acagaaggtt gatcgatcca gccgatcaga tctttcggag caccggcagc gatagcagcc
    16621 tgcagaacga tatcagccgc tttgttggtg gcatcttttg cacgcgggtg cggggagaag
    16681 ataatggcgt tacgggtctt cagactgatc agcgatttga agatagcagt tgaagtcggg
    16741 ttagtggtcg gaacgatacc gcaaataata ccgattggtt cagcgatagt gatggtacca
    16801 aaagtgtcgt cttcagacag aacaccacag gttttttcat ctttataggc gttgtagata
    16861 tattcagaag caaagtggtt tttgatcact ttatcttcga cgatacccat gccggattcg
    16921 gcaacggcca ttttcgcgag tgggattcga gcatctgcag cagccagagc ggcggcgcgg
    16981 aagattttgt ctacttgctc ttgagtgaaa ctggcatatt cacgctgggc tttttttaca
    17041 cgctctacga gtgcgttaag ttcagcgaca ttagtaacag ccataatgct ctcctgataa
    17101 tgttaaactt ttttagtaaa tcatctgctc gaatacgaga gtatagtcag tgcggtgatg
    17161 atttgcttaa ccgatgaaaa tcaaaagctt actcgcgctc acactcactg tgatttacta
    17221 aaagagttta aacatcagag ttattatctc taatgcgtca cttccaggtg gcgtaagcaa
    17281 gattactcac ttctgggtac tgattacgtg atccaaatca aatttttgca aagctgacac
    17341 ctttcagcat cgcttttcgc cattatagct aacagttaat aaattgtagt atgatttggt
    17401 ggctacatta gcatgttttg cacaactaga taacaataac gaatgatagc aattttaagt
    17461 agttaggagg tgaaaaatgc tgtcaaaagg cgtattgtca gcgcgtcttt tcaaccttat
    17521 ttatggctaa cattatccgg cttttgcttc ggagctaacc gtgattcaga ccctttttga
    17581 ttttcccgtt tacttcaaat ttttcatcgg gttatttgcg ctggtcaacc cggtagggat
    17641 tattcccgtc tttatcagca tgaccagttt tcagacagcg gcagcgcgaa acaaaactaa
    17701 ccttacagcc aacctgtctg tggccattat cttgtggatc tcgctttttc tcggcgacac
    17761 gattctacaa ctttttggta tatcaattga ttcgttccgt atcgccgggg gtatcctggt
    17821 ggtgacaata gcgatgtcga tgatcagcgg caagcttggc gaggataaac agaacaagca
    17881 agaaaaatca gaaaccgcgg tacgtgaaag cattggtgtg gtgccactgg cgttgccgtt
    17941 gatggcgggg ccaggggcga tcagttctac catcgtctgg ggtacgcgtt atcacagcat
    18001 tagctatctg tttggtttct ttgtggctat tgcattgttc gctttatgtt gttggggatt
    18061 gttccgcatg gcaccgtggc tggtacgggt tttacgccag accggcatca acgtgattac
    18121 gcgtattatg gggctattgc tgatggcatt ggggattgaa tttatcgtta ctggtattaa
    18181 ggggattttc cccggcctgc ttaattaatt cctttcaaat gaaacggagc tgccatgctc
    18241 cgtttacttc gtcattattt ttactttgtt cccgcgcagt tatcaaaagc aaaaggaata
    18301 ggtaaaaata ttcttctcaa attacagtta gttataagga tttccttaac tgtttctctt
    18361 caccatcatg ttattttcgc cacatcataa tcctgggctt gctgaagaat aattgaaatg
    18421 atattagtaa ttccactgcc tttggtagag gaaagtgcta aataataatc aattgttaaa
    18481 tgattgtgca tttcactact ggaactgtaa tcagaaaaga tagacatgct taaccaatct
    18541 ctatttgatt gaattgaaag atgtttgtta agacatggat gcaagctata gattctgata
    18601 cggtcaataa aagagaattg cttaacaatt ttgcaaaatg tattggcgag taagaagcgc
    18661 atttggtact ttccgggcaa ccgccaggcg attctttatt ggtaatgaga ataattaaca
    18721 attaaagagc gtcgcgaaag aataatgtgt ctcgacaggg gagacacagt acgaatcgac
    18781 ataaggtgat cgtctgaatc accagaataa ataaagtcgg tgatagcaat ccgtaacgat
    18841 aaagtaacct gacagcagaa agtctccgag cctgtgcagg gtcccattcc gggattacac
    18901 atgctggtta ataccagtaa ttataatgag ggagtccaaa aaacaatgac caacatcacc
    18961 aagagaagtt tagtagcagc tggcgttctg gctgcgctaa tggcagggaa tgtcgcgctg
    19021 gcagctgatg tacccgcagg cgtcacactg gcggaaaaac aaacgctggt acgtaacaat
    19081 ggttcagaag ttcagtcatt agatccgcac aaaattgaag gtgttccgga gtctaatatc
    19141 agccgagacc tgtttgaagg cttactggtc agcgatcttg acggccatcc ggcacctggc
    19201 gtcgctgaat cctgggataa taaagacgcg aaagtctgga ccttccattt gcgtaaagat
    19261 gcgaaatggt ctgatggcac gccagtcaca gcacaagact ttgtgtatag ctggcaacgt
    19321 tctgttgatc cgaacaccgc ttctccgtat gccagttatc tgcaatatgg gcatatcgcc
    19381 ggtattgatg aaattcttga agggaaaaaa ccgattaccg atctcggcgt gaaagctatt
    19441 gatgatcaca cattagaagt caccttaagt gaacccgttc cgtacttcta taaattactt
    19501 gttcacccat caacttcacc ggtgccaaaa gccgctatcg agaaattcgg cgaaaaatgg
    19561 acccagcctg gtaatatcgt caccaacggt gcctatacct taaaagattg ggtcgtaaac
    19621 gaacgaatcg ttcttgaacg cagcccaacc tactggaaca acgcgaaaac cgttattaac
    19681 caggtaacct atttgcctat tgcttctgaa gttaccgatg tcaaccgcta ccgtagtggt
    19741 gaaatcgaca tgacttataa caacatgccg atcgaattgt tccagaagct gaaaaaagag
    19801 atcccggacg aagttcacgt tgatccatac ctgtgcactt actattacga aattaacaac
    19861 cagaaaccgc cattcaacga tgtgcgtgtg cgtaccgcac tgaaactagg tatggaccgc
    19921 gatatcattg ttaataaagt gaaagcgcag ggcgacatgc ccgcctatgg ttacactcca
    19981 ccgtatactg atggcgcaaa attgactcag ccagaatggt ttggctggag ccaggaaaaa
    20041 cgtaacgaag aagcgaaaaa actgctggct gaagcgggtt ataccgcaga caaaccgttg
    20101 accatcaacc tgctgtataa cacctccgat ctgcataaaa agctggcgat tgctgcctct
    20161 tcattgtgga agaaaaacat tggtgtaaac gtcaaactgg ttaaccagga gtggaaaacg
    20221 ttcctcgaca cccgtcacca gggtactttt gatgtggccc gtgcaggctg gtgtgctgac
    20281 tacaacgaac caacttcctt cctgaacacc atgctgtcga acagctcgat gaataccgcg
    20341 cattataaga gcccggcctt tgacagcatt atggcggaaa cgctgaaagt gactgacgag
    20401 gcgcagcgca cagctctgta cactaaagca gaacaacagc tggataagga ttcggccatt
    20461 gttcctgttt attactacgt gaatgcgcgt ctggtgaaac cgtgggttgg tggttatacc
    20521 ggcaaagatc cgctggataa tacctacacc cggaatatgt acattgtgaa gcactgatgg
    20581 caatacgtgg ggcaggagtg tcctgctcca cggtgtctga tttttatcgc attacagaag
    20641 gcacaggcca gaaggtaggg caatgttaaa atttatttta cgtcgctgtc tggaagcgat
    20701 tccgacgcta tttattctta ttactatttc gttctttatg atgcgcctcg cgccgggaag
    20761 tccttttacc ggcgaacgta ctttaccgcc agaagtgatg gccaatatcg aagcgaaata
    20821 tcatcttaat gatccgatca tgacacagta tttcagctac ctgaaacaac tggcgcacgg
    20881 tgatttcggt ccatcgttta aatataaaga ttattcggtc aatgacctgg tggcatccag
    20941 ttttcccgtt tctgccaaac tgggagccgc agcatttttc cttgcggtaa tactgggtgt
    21001 tagtgctggc gttattgccg cattaaaaca aaacaccaaa tgggactata ccgtgatggg
    21061 gctggcaatg accggggttg ttatccccag ttttgtggtt gcgccattat tagtcatgat
    21121 atttgcgatc attttgcatt ggctgccggg cggtggctgg aatggtgggg cgcttaaatt
    21181 catgatattg ccgatggtgg cgttgtcact cgcctatatc gccagtattg cgcgtattac
    21241 ccgtggctct atgattgaag tattacactc caactttatt cgtactgccc gggcgaaagg
    21301 gttacctatg cggcggatca ttttacgcca cgcattaaaa cctgctctgt tacccgtgct
    21361 ctcctatatg ggccccgcat ttgtcggcat tattaccggt tctatggtta tcgaaaccat
    21421 ttatggtttg ccggggattg ggcaactgtt cgttaatggc gcattgaacc gtgactattc
    21481 cttagtgtta agcctgacca tcctggttgg tgcgttaacc attttgttta atgccattgt
    21541 cgatgtgcta tatgcggtta tcgacccgaa aatccgttac tgatactgga gctcgcgatg
    21601 atgttaagta agaaaaacag cgagacgctg gaaaatttca gtgaaaagct ggaggtcgaa
    21661 gggcgcagct tgtggcagga cgcacgtcga cgttttatgc ataaccgtgc ggcggttgcc
    21721 agtctgatag tgctggtgct gatcgcgtta tttgtaatcc tggcaccgat gctttcgcag
    21781 tttgcctatg acgatactga ctgggcgatg atgtccagcg ccccggatat ggagtccggt
    21841 cactactttg gtactgactc atccggtcgc gacctgcttg tgcgcgttgc gattggcggg
    21901 cgtatctcac tcatggtcgg tgttgctgcg gcactggtgg cagtggtcgt ggggacactt
    21961 tacggttcgc tttccggtta tctgggcggt aaagtggatt cggtaatgat gcgtctgctg
    22021 gaaatcctca actccttccc attcatgttc ttcgtcattt tgctggtgac ctttttcggc
    22081 caaaacatcc tgctgatttt cgtggcgatt ggcatggttt cctggctgga tatggctcgt
    22141 attgtgcgtg ggcaaaccct gagtctgaag cgcaaagagt ttattgaggc ggcacaagtt
    22201 ggcggtgtat cgacgccggg cattgttatt cgccacattg tgccgaacgt actcggtgtg
    22261 gtggtggtct acgcatcgct actggtgccc agcatgatcc tctttgaatc tttccttagc
    22321 ttcctggggt tgggtacgca agagccgtta agcagctggg gggcattgct gagtgatggc
    22381 gcgaactcga tggaagtctc tccatggtta ctgttgttcc cagcgggatt cctcgtggtg
    22441 acgctgtttt gtttcaactt tatcggcgat ggcttgcgtg atgccctcga cccgaaagat
    22501 cgttaaggag tgcagccatg agcgtaattg aaactgcaac tgtgccgctc gcacaacaac
    22561 aggctgacgc actgctgaac gtgaaagatt tgcgtgtcac ctttagtacc ccggacggcg
    22621 acgtcacggc ggtcaatgat ttaaattttt ccctacgtgc cggagaaacg ctgggcattg
    22681 taggtgagtc tggttcgggt aaatcgcaaa ctgcatttgc gttgatgggc ctgttggctg
    22741 ccaacggacg cattggcgga tcggcaacct tcaatgggcg tgaaatcctc aatttgccag
    22801 agcgtgaact caataaactg cgcgctgaac aaatctcaat gatttttcag gacccaatga
    22861 cttcgttgaa tccctatatg cgcgtcggtg agcagttgat ggaagtgctg atgctacata
    22921 agaacatgag caaagctgag gcgtttgaag agtcggtgcg gatgctcgat gcggtaaaaa
    22981 tgccggaagc acgtaaacgc atgaaaatgt acccgcacga attttctggt ggcatgcgtc
    23041 agcgagttat gattgcgatg gcattgctat gtcgacctaa gctgctgatt gcagatgaac
    23101 ccactactgc gctggacgtc accgtacagg cgcagatcat gacgctattg aatgaactga
    23161 agcgggaatt taataccgcc atcattatga tcacccacga tcttggcgtg gtggcgggga
    23221 tctgcgacaa agtgctggta atgtacgccg ggcgcacgat ggaatatggc aacgcgcgcg
    23281 atgtctttta tcaacccgtt catccttatt ctatcggttt gctcaacgcg gtgcctcgtc
    23341 tcgatgcgga aggtgaaaca atgttgacta ttccgggtaa tccgccaaac ctgctgcgat
    23401 taccgaaagg ttgcccgttc cagccacgtt gtccgcatgc gatggaaatt tgtagtagcg
    23461 caccgccgct ggaagagttt acgcctggcc gtctgcgtgc ttgctttaaa ccggtggagg
    23521 aactgttatg aatgctgtaa ccgaaggaag aaaagtcctc ctcgaaatcg acgatctgaa
    23581 agtgcacttt gaaatcaaag atggcaaaca gtggttctgg caaccaccga aaactctcaa
    23641 agccgtagat ggtgtaactc ttcgcctgta tgaaggggaa acattaggtg tggtagggga
    23701 atcgggatgc ggtaagtcca cctttgctcg cgccatcatc ggtttggtca aggcgaccga
    23761 cggtcatgtt gcctggttag gtaaagagtt gctgggcatg aagcccgatg aatggcgtgc
    23821 cgttcgcagt gatattcaga tgattttcca ggatccgttg gcatcgctaa acccgcgtat
    23881 gaccatcggc gagatcattg ctgaaccact gcgtacttat catccgaaaa tgtcacgcca
    23941 ggaagttcgc gagcgcgtga aggcgatgat gctgaaagtc gggttattgc ctaacctgat
    24001 taaccgctat ccgcatgagt tctccggtgg gcagtgccag cgtatcggga ttgctcgtgc
    24061 tcttattctt gaaccgaagc tgattatctg cgatgagccg gtttcggcgc tggatgtgtc
    24121 aattcaggcg caggtggtca acctgctcca gcagctgcaa cgtgagatgg gattgtcatt
    24181 aatttttatt gctcatgacc tggccgtggt aaaacacatt tccgatcgtg tgttggtgat
    24241 gtatctcggc catgcggtag aactggggac ctatgatgag gtctaccaca atccactaca
    24301 tccttacacc agggcattga tgtcggcagt ccccatacct gatccggatc tggagaagaa
    24361 caaaaccatc cagttactgg aaggggaatt accgtcgccg atcaacccgc cttccggttg
    24421 tgtttttcgt acccgttgcc cgattgccgg tccggagtgt gccaaaacac gtcctgtgct
    24481 ggaggggagt ttcagacacg ccgtttcttg cctgaaagtc gatccgcttt aaacacaaaa
    24541 agggctgaca gttgtcagcc ctttttcacg ccgaaagtga ttatttattc ccgccagatg
    24601 atatggcaaa gtttgtgatc tttttcgcgg cataacagaa tgcgggcgaa aacatcgttg
    24661 atttcaccat cttcactgtc cgccagacca atcaccactt cggcaaaaaa gtctgggttc
    24721 aaatcgaaat caacatgttc ctgccagtct tccgccggat cgaataactc cgcgccgccg
    24781 cgctcttcaa actgaagatt gaacagcaga acatcagctg gatcgagatt gtcggcagcc
    24841 agttcgagaa aaatatcgta ggcctgctca agcgtttcat cttcagtcag gcgattgttt
    24901 agatccatat ccatagtcac tacctgttta acctctgttg gcgacgtttt acagcaacgg
    24961 gctgaagaag taaaacagtc gctcggcgac acgttgccat aatggacgtt ttagccataa
    25021 acgggcatcg agcagacgtg aacgcgaaat ataatcgtcc tgaacggcgg caaggtctgc
    25081 accaaaacct ttatcgtcga ttgccagggt aatctcgaaa ttcagccaca gactacgcat
    25141 atcaaggtta acggtgccaa ccagacttag ttcgccatcg accagcacgc tcttggtatg
    25201 cagtaacccg ccttcaaact gataaatttt aaccccagca gcaagcagtt ccgtaaagaa
    25261 tgcgcgactg gcccagccga ccagcatcga gtcatttttt cgcggaagga taatactgac
    25321 atccaccccg cgctgcgccg ccgtgcaaat cgcatgaagt aaatcatcgc ttggcacaaa
    25381 gtagggcgtg gtcatgatca aatattcacg cgccgaataa gccgcagtca ataatgcctg
    25441 gtgaatgaga tcttccggaa agccggggcc agaagcaatt gtgtgaatgg tatgaccgct
    25501 ggcctgctca aacggcataa tattgacatc aggtggtggc ggtagaatac gtttccctgt
    25561 ttcaatctcc cagtcgcagg aataaataat ccccatcgcg gtggcgatgg ggccttccat
    25621 acgcgccatc agatcaatcc attgccctac gcccgcatct tgtttgaagt agcgaggatc
    25681 gaccatattc atgctgccgg tgtacgcgat gtaattatcg atcatgatca tcttgcgatg
    25741 ttggcgcagg tccatacggc gtaaaaacac acgcatcaga ttgaccttta aggcttcgac
    25801 cacttcaata ccggcattac gcattagctc gggccacggg ctgcggaaaa aagccacact
    25861 cccggcggag tcgagcatca atcggcaatg aatgccgcgt cgcgcagccg ccattaatga
    25921 ttcagccacc tggtccgcca tgccgccggg ctgccagata taaaacacca tctcaatatt
    25981 atggcgcgcg agctggatgt cgcggattaa cgcctgcatc acatcatctg actcggtcat
    26041 cagttgtagc tgattccctt tgaccccagc gatcccctga cgacgctcgc aaagcttgaa
    26101 taatggcgca gcgacactgc tattttcttc ggcgaagata tgcttacagg ctttaaggtc
    26161 gttaagccat tttgcggtgg aaggccacat cgctctggcg cgctcagcgc ggcgtttgcc
    26221 taaatggagc tcgccaacgg caagataggc aataattccg actaacggca gaatgtaaat
    26281 aatcaacagc caggccatcg cggagggaac tgcgcgtcgt ttcattagaa tgcgtaaagt
    26341 tacgcctgca atgagcaacc agtatcccag aatggccaac caactcacca acgtataaac
    26401 ggttgtcata gattagaaat ccttttgaaa gcgcattgtt atgagtttac gcatatggat
    26461 tcatctggca aataaaaacg cgggaaaagc actggtcagc gtcgggggcg ggggtataat
    26521 gaccattctg ttattgcata gagtagttaa catgaagcgg agtagaacgg aagtggggcg
    26581 ctggcgcatg cagcgtcagg ctagccgacg taaatcgcgt tggcttgagg ggcaatcgcg
    26641 ccgaaatatg cgtatccaca gcatcaggaa gtgcattcta aacaaacagc gtaactcgtt
    26701 attgtttgcg atctacaata tctaaatgtt ctgggcacca ttgcggtgcc caataagttt
    26761 acttttccgt ctttaactct atttttgcac cgattcttta ctgtcagctt tcgtttcttg
    26821 ttcatcgtta tcgctgaaaa tgccatgacc ggagttcaac aacattgaaa cgagcatatc
    26881 attattaatc tcttcaccat ttaaaactcg cgccagaatt tcgctgccaa acagttgcgg
    26941 tgagagaatg atatccggat gtaccatctt aatcttattc aggtttttgc tatcactgac
    27001 ggcgagaact gttttgacat cactgctcat atcttttgcc gagagtacaa caaacgcgtt
    27061 atctgcatcg ttatcactca gcgccagaat ggcccggcaa cgatcgattc ccgctttctt
    27121 taatacagaa ctgtcattac tgtcaccggg gataacgtct gcgttatcgc ctaagcgttg
    27181 ctcaagttgc ttgatatcat cttcaggcaa gttgctgata accgttacgt tttgtccgcg
    27241 ttgattcagt tgcaggatcg tattgatggc gagaatcgaa tgtccgcaaa caataaaatg
    27301 atctttacga tgcattgtat gattgtttcc ttttacaagt ttgttgaatc ccccgcggat
    27361 aagcggacca aaaattgagg tcatagatgt ggcaaaaacg gtaatgccgg aaataatgac
    27421 cgaaatagtg aacaatcgtg ctgattcaga aacagggaca atatcgccat agccgacggt
    27481 tgacatggtt tctatcgaaa aatagaacgc ggtcatcaaa ctttctattc gcggattaaa
    27541 accttcgctt aaataaagcg caccgtaggt tgagtaaaac agtaatgtcg tgaaactaat
    27601 aaatgcaaaa attgtcccgg ctgcggcgct actgtgggag aagtctttgc gcagtatcag
    27661 caaaaacacc agcgtaaaaa tgcaaaatcc aatactaaat ttcagccagg gataaaaatg
    27721 cagggtgtaa attagcgcta tcaacaacaa aatgatactg attgcccatg cgagctttgc
    27781 ccggaacagt aagccaatgg agttcaggac aagaaagacg ccgagcataa atagtggggc
    27841 gtttgccagc aaggagagat ccaactctga aaatgctttg atatggaaaa tatcaagcaa
    27901 attgaccgac ataccataga ttgttttaaa aataagcaat ccatttaaaa agacggccag
    27961 cgcgagaatg tcgtgccgta gggtcaccca taaatttgtt gctgtttgtt tgaatgtagc
    28021 ccagtgactc actgatacct ctgtaaccac aattttgttt aattatctta gcgtgcacat
    28081 tttctgaaca tacatgcagc gcgtgaatgt gttaaacaat agtagatgac tttatccgca
    28141 gtaatcaaag gttttgagtg agcaactctg cgtcaggaaa gggcattttt cctgaatatg
    28201 ctcacatcat ataaagaaat acagataaag ttattatctg cttgtggtgg tgaatgcact
    28261 gaccgaccta taaggaaagg ccaaacaaga acacggttgc aaaaaccgtg cccttaaata
    28321 ttggctctct actcagaaca ctttcttaaa tggtttcact gaaacgtgtt catagactcc
    28381 tgccgctacg tacgggtcag catcggccca ggcctgagct gcttccagcg attcaaattc
    28441 agcaataacg gttgagccag taaatcccgc agcccctgga tcgttactgt ctaccgctgg
    28501 cattggacca gctgtcaaca aacgaccttc atcatgcagt aactgtaaac gtgctaaatg
    28561 tgccggacga acggaaaggc gtttttcgag gctatcagct ttgtcttgag cgtaaataac
    28621 atacaacacg ggcacaactc cttgttcggg aaagttgtaa agtacgttat ttgaaagggc
    28681 gaagatctgc aacggaaaga tgatgtcttt gttaagacca tgcataaagt aaggtcaatt
    28741 acgccaaaaa tgacattttc actgatcctg atcgtcttgc cttattgaat atgattgcta
    28801 tttgcattta aaatcgaggc ctggtttttc tactgaaatg attatgactt caatgaccct
    28861 tgatttacct cgccgcttcc cctggccgac gttactttcg gtctgcatac atggtgctgt
    28921 tgtggcgggt ctgctctata cctcggtaca tcaggttatt gaactacctg cgcctgcgca
    28981 gccaatttct gtcacgatgg ttgcgcctgc tgatctcgaa ccgccacaag ccgttcagcc
    29041 gccaccggag ccggtggtag agccagaacc ggaacctgag ccgatccccg aaccgccaaa
    29101 agaagcaccg gtggtcattg aaaagccgaa gccgaaacct aagccaaaac cgaagccggt
    29161 gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc
    29221 accgtttgaa aatacggcat cggcacgccc gacatcaagt acggcaacgg ctgcaaccag
    29281 caagccggtt accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta
    29341 tccggcacga gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgatgtcac
    29401 gccggatggt cgcgtggata acgtgcaaat cctctcagct aaacctgcga acatgtttga
    29461 gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg
    29521 gattgtggtg aatatcctgt ttaaaattaa cggcaccacc gaaattcagt aagcagaaag
    29581 tcaaaagcct ccgaccggag gcttttgatt attactcaac aggtaaggcg cgaggttttc
    29641 cttcaggatc aaccgcgaca tacttaaata atgcttctgt cgctttatag cgttgcccga
    29701 ttggttcaga cgctactttt ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc
    29761 ctttctggac acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga
    29821 aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg ggcaatttct ttcgccagaa
    29881 tagcgccgcc aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat
    29941 tggtatcggc gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg
    30001 ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt
    30061 tcctgaggag aacagaggag atacagcgcc agccccgaag gactggcagt ctggttagga
    30121 tttatcttcc tgcggcatgt ggcggtagat atagataccg cttaacaatg tgaagattaa
    30181 ggtaagggcg gtcaggccaa agactttaaa gttgacccaa atattttgcg gcagccagaa
    30241 tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa acagcccagg ccagattcag
    30301 cttcgaccat accggttgcg gcagcgtgag ttctttaccc agcatccgct gaattagcgg
    30361 ctttttcatc acccattggc tgactaacag ggcacccgca aacagggcat aaatgacagt
    30421 aaccttccat ttaataaact catcattgtg gaagaacagc gtcaagccac cgaagacgac
    30481 caccagaaca aaagtgatca gggccatctt ctcaacctta cgaaagcgaa cccagctata
    30541 gataagcaca atcgccgtgg cgacgatcag cgccgcagta gccgcataga tgtcataaat
    30601 cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca agaaactgct tcattttacg
    30661 attccgtaat caaatgctta aggttattgg cgaatcaaca tatacagacg gaacagatag
    30721 atgagcaata cggctgaaat caggttgctc aaggtgttcg ccagtacggc accaatttcc
    30781 ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca gtgtctttgc cagcaaccag
    30841 ctcagtactg cgggcgccac cagacgcata ttcgcccaag tcagccgcat actgctacgc
    30901 atcgaggcaa aaacgcccat tttgtcctgt accaacatca ccggtgccag ggccagtaaa
    30961 atggccatga taattcccgg aacgaccact agcatgatgc caatctgtac taaaagggta
    31021 gtcagaaaaa tcagaataaa taactttggc aatatcggcg cactggcacc aatagctcgc
    31081 agcgcactga ctctctgacc cgcagacacc agctggataa ttaatattac gcctccggcg
    31141 agaatggcgt taccgattaa tcctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt
    31201 tgttgctgtt ccggtgacat attctgaacc aggtcgaaca acccactact gccgctaacg
    31261 ggcacgccgt cattgagctg cgcaagctgt gcatcactgg gtgagaaaac atgccctaac
    31321 accactgtga taaacgcaca tagcaacgat accaacagaa tggtcataaa ttgattacgg
    31381 aagaaatttc cggtgtcacg gtatacggac tgcgccgtga tagacatgca ctctccttga
    31441 gttttatgca ggtgttaatt agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca
    31501 tcaggtcttg tatggaaaag catatctttg taaagcggag gtaattgctg gccttcaggc
    31561 gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg cgtagcgaaa atatttaaaa
    31621 attgatttaa atcacgttaa ccaggattcc caatgcaact tctaaattaa tccagatcaa
    31681 taaagggtga attatcatat gtaatgtgat ctatgtagga tcatttgtta ctccaatgta
    31741 ggtatatttg tcacgttttt ataaccataa cgacggagcg gatatgaaaa agttaacagt
    31801 ggcggctttg gcagtaacaa cacttctctc tggcagtgcc tttgcgcatg aagcaggcga
    31861 attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca gaaggtgctg gtggtacgtt
    31921 aggaagtctg ggtggattca gcgtgaccaa taacactcaa ctgggcctga cgtttactta
    31981 tatggcgacc gacaacattg gtgtggaatt actggcagcg acgccgttcc gccataaaat
    32041 cggcacccgg gcgaccggcg atattgcaac cgttcatcat ctgccgccaa cactgatggc
    32101 gcagtggtat tttggtgatg ccagcagcaa attccgtcct tatgttgggg caggtattaa
    32161 ctacaccacc ttctttgata atggatttaa cgatcatggc aaagaggcgg ggctttccga
    32221 tctcagtctg aaagattcct ggggagctgc cgggcaggtg ggggttgatt atctgattaa
    32281 ccgtgactgg ttggttaata tgtcagtgtg gtacatggat atcgatacca ccgccaatta
    32341 taaactgggc ggtgcacagc aacatgatag cgtacgcctc gatccgtggg tgtttatgtt
    32401 ctcagcagga tatcgttttt aattccgcac aaaaacgacc ccgtaatata cggggtcaat
    32461 aaggacatgg cataaagggt attatttctt cgcttctacg ccatcagttt cagagcgaat
    32521 taaaaatttc tcagttgttt gcggaatatg ctgaatcagc cagtcggcca tttgcttttc
    32581 ctcatttaaa atcgcttcga tgatcggaat tgaggctgta tcaccggcat tttttgctgc
    32641 tgctaataga gatgtgtaac aggcgatttc aaattgctcg aagacatatc cgctaataga
    32701 acctttgact atttcatcag aagggaatat accaccgatt gactgcccaa gcgcagccat
    32761 tttactcatg gaatctttaa tgactgaacg tgaaatgtca ttacgatcaa gaatagtttc
    32821 cagttgaaca atctggtttt tggtttcact aagatgttgt tcaatacgag cacgtagttc
    32881 aggataatta tctatacggc tggccatgga ttcaagcata gattcggctt gcttttccat
    32941 tgcatgggcg tcacgtaacc agtcatgata atgttcaata cgattcatga aaatgtcctc
    33001 tttataaaat gactaaaagt taaaattcat atttcaggct ttattttcgg ctttcttatt
    33061 tacgttatta agggccagat cagtcagttt gatgtcggtg gccttttctt cttccagggt
    33121 ctctttcaga agcttcgctg ctttacggta acctaattgt tcagccagcg tcgctaatgt
    33181 cccgtaactg gcaatctcat aatgctcgac tttctgtgct gcggcaatca gtgcggcatc
    33241 acgcacttcg tttttctcgg tactttcgat gacctcatta gcttcttcaa taagaccttc
    33301 cattgccaca catttcatgc gcttaatttt cagattcgat tctgattcca caacttgatc
    33361 aatacgttca atctgtccat gtgtttcctc gaggtgcgca tgaaaagcct gacttaattt
    33421 ttcgtttgat gttgctcttg cgagttttgc cagtgcccgg gttaattgtt tttctgcgct
    33481 gtaggtatct gaaagtaggt gaataaatac atcttccatg gtcttcatat tcatattttt
    33541 ctccagtgga atcactgcga gccatacggc ccgcagaagc aattatcttt atcagctgat
    33601 attaataatt aacggtgaat caggatttac ctgatttatt accaccgttt tgttgaccgc
    33661 cttttttacc cgcttcagat gcgcgttgcg gatcattttt aaaattaccg ccgctatgct
    33721 gaccgccttt acggcctgcg tcggatgcct tctcacggtc ttcggcgaaa tttcctgaac
    33781 caccacgatg ttcggccatg ttatttctcc cgttgcgttg cattgtttca ttaatatgag
    33841 tgttgtgtgt cgacactcat taaaattagt cgataatgag aattggtcaa attaagcgta
    33901 acgagaagga agtgggaaaa tatattatga ggaacgttct tgatatatta acaattgcct
    33961 gaggaataag tgacttagag aaaattattg attttactgg tgttatgttg cgggattaat
    34021 ttgtttgatt atattactgt tgggcggaaa aatgacgtaa gttgacgttc gacaggggta
    34081 agcgaaacgg taaaaagata aatattaaat gaatttagga tttttccggc ttcgttaaag
    34141 aaagttaaaa tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt
    34201 catcggttgt acaaaagctt tcagtgccgc cagcattttc tctggctcat taatatgttg
    34261 ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc
    34321 tgcttttacc tgatccgggg cggaaatacc aaatccctgc aatggaggtg cagcgttgta
    34381 ctctttcagc ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt
    34441 cacgcccgct cgtgacagca aatatgtgta accacgaccg taagaggcta tctggcgcag
    34501 caggtcgtca tcggcatttg gcgggcagat gaagataggt gcgacattat gacgcaacgc
    34561 ggcctggcgg aagggcgcgg actcttcaac tggcacatcg gcaaccagca ccgaatcgac
    34621 gccgactttt tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccagattggc
    34681 atacatcaac aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc
    34741 aaaacattgt gccggagtca cacctgccgc aaaggcgcgc agagtggcgt tttgaatggt
    34801 cgggccatcc gccagtgggt cggagaaggg gatacctaac tccagcgcgt cagcaccggc
    34861 ttcaattagc gtatcgataa ttttcaatga ctgctcaatg cccggatccc cgagcgtgac
    34921 gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg
    34981 ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg
    35041 ccgcgaccgg aaaggttaac caccagtagc tgctcttttt ccgggttttc gcgcatcatt
    35101 ttcagtgcat gggccagggc gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg
    35161 cacagcgttt tgaaggcttc aagggcttca tcatcggtaa tagacacgta atcagcgcgt
    35221 ccagtgctgt taagatacgc gtgttgtggg ccgacggacg ggaaatccag cccggcggag
    35281 atggagtaag attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg
    35341 aaatagatgc ccacgcgacc atgttttaac ggtgcgccgt gctcgccgat ttctataccg
    35401 tgaccgccag gctccacacc aatcaggccg acgttggttt cgttgatgaa atctgcaaac
    35461 atgccgatgg cattcgaacc gccgccaaca caggcgataa cggcatccgg caggcgacct
    35521 tctctttcca gaatctgtgc tttggtttct tcgccaatca tccgctgaaa ctcacggaca
    35581 atggtcggat aaggatgcgg gccagctgcg gtgcccagca tatagtgcgc ggtttcataa
    35641 ctgccggacc agtcgcgtag cgcctcgtta caggcatctt tcagcgtcgc ggaaccgcta
    35701 tgcaccggga tcacttccgc acccattaag cgcatacgaa aaacgtttgg cgactggcgt
    35761 tcaacgtctt tggcccccat ataaatacgg catttcaggc cgagcagggc gctggcaagg
    35821 gccgacgcca cgccatgctg accggccccg gtttcggcga tgatttcggt tttacccatc
    35881 cgcttcgcca gtaaagcctg accgagcacc tggttagttt tatgcgcgcc gccgtgcagc
    35941 aaatcttcgc gcttcagata cagcgtggtt ttcgtcccag ccgtaatgtt ctggcatttg
    36001 gtcagtgcgg ttggacgccc ggcatagttt ttcagtaggt cgttgaactg ggcctgaaat
    36061 tcaggatctt tttgcgcgct gacaaaagct tcttccagct ggcgcagagc aggcatcagg
    36121 atttgtggca cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt
    36181 gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt
    36241 tgatgcccgg ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg
    36301 ccgcttccac gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt
    36361 gaccgtttaa cagtgaccag tcgaaacgtt gcccgcttcc accctggccg ttgtctaaaa
    36421 catatttatc aacgtgctga agctcgcggg cgggcagggt ttcaccgacg cttaatgctt
    36481 tccagatggc aacgtgtgct ggcagagctt cacgcagcgt atcgatatac agctgatctt
    36541 cattaccatg cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg
    36601 caatatcgtg attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct
    36661 gcgcctgttc aatgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa
    36721 tcgcgcccgc gtcatacgct gctttagcat cttgcccacg cgtcaggcca catactttat
    36781 tctcacccag caacacccgg cgcacggcgg cgttcaaatc gtcatgggcc atcaacgccg
    36841 aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga
    36901 tgcctgattc gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac
    36961 ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg acctttgccc
    37021 ccaatgcaat ggcgcgctcc agttcctctt cattactgac ttcggtcagc acacccatct
    37081 ccagactgtg ggcgacggcg gcaagctggc gatattgttc gtcatccagt actgaaagca
    37141 ttaataagca ggcatcggcc tggtaatagc gcgccagata gatctggtaa gggtcgataa
    37201 tgaagtcttt acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa
    37261 agctcccctg aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatgtttat
    37321 aaatggcggc aatgcgtgct ggatcgaaat catcacggat cacgcctttt gacggcgacg
    37381 ctttcttgca ctccagaata aacgccgtgc gcgcaccctg tagcgcatca taaaaatgtc
    37441 gcgtgctcgg ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggctt
    37501 ctacccaaat cgccttgtct gcgacgatct tcgctaaaac ggtttgcatc atttaccctc
    37561 gtgccgccag tgcggtgact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg
    37621 tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacgttcg
    37681 cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg taacaaacgt gttaaaatgt
    37741 cacggttttc ttccggtgtt ccgcctgcca gttgctcctg gtggtagggc gtcaggccaa
    37801 aatcttcagc ggtcaattga tagctcttaa tttcgccgtc atgcagctcg gcaacgattg
    37861 tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat
    37921 accccagcac gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa
    37981 ttaacgccag cggcggatgc gccgggttaa tcaatggtcc cagcacgttg aacaaggtgc
    38041 gggttttcag ttgctggcga accggcatcg cgtggcggaa tccggtgtga tacttcggcg
    38101 caaagaggaa acagacgcct aattcgtcca gcgcctggcg cgatttatcg gcgttcatat
    38161 caagattaat accgaatgcc gccagcagat ccgacgagcc agatttactg gagacgctac
    38221 ggttgccgtg tttcgcaact ttcagcccac aggccgcggc gacaaacgca ctggcggtgg
    38281 aaatattgat actgttgctg ccgtcaccgc cagtcccgac gatatcagca aacagataat
    38341 ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt tgctgctccg gcgatctcgt
    38401 tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggcttca
    38461 gctcgccacg caccaccgct gaaaacagct ggtgactttc ttgttggcta agcgtctgcg
    38521 cctgatacag tttttccaga atcggttgca gcgtgttggt tggctcaagt ttctgctgcg
    38581 cccaggccag cgtttgttcc agcaggcgag cgccctgggt agtaagaatg gattccggat
    38641 ggaactggaa tccacaaacg cgatccgcat cgtgacgtac tgccatcacc atgccattaa
    38701 aatgggcatt gatggttaaa cctgccggaa tgttactgcc aaccagcgag tgataacgcg
    38761 ccaccggcaa tgggtttgtt aatccggcaa acatcgcctg accgtcgtgt tcaatgctgg
    38821 aggctttacc gtggagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa
    38881 tcgcctgatg accaaggcaa atgccaataa tcggcagctt accgcgcagg cgggtaagca
    38941 gttctggcat acaaccagct tcgctcggca caccgggtcc tggagaaagc atcagcaccg
    39001 gattgctcat cgtcgccagg cgttcaatta aggtttgcgc cggaatatgg ttgcggtaaa
    39061 tcaccacgtt atgcccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga
    39121 tattatcgag cagcagaatg tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc
    39181 aatagcgcgc agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac
    39241 agaatcaagg actacgccag caccggcttg cacggtggcg ataccgtttt ccaccagcgc
    39301 cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc
    39361 gccgtagctg ccgcggcgac gaccttcagc ctcggcaatt aactgcatag cgcgtacttt
    39421 cggcgcaccg cttaacgtac ccatattcat acaggcgcgg taagcgtgta gggcgtcgag
    39481 atcgtggcgc agctcaccga ccacgcggga gaccaggtgc atcacgtaag agtaacggtc
    39541 aactttggta agatcggcga cgtagcggct gccgggggtg caaatgcgtg ccagatcatt
    39601 acgggcgaga tccaccagca tcagatgttc agaaagctct ttatgatcgg tacgcatttc
    39661 cagttcgatg cggctgtcga ggtctctgtc cagtgaacca tcggcacgac gaccgcgtgg
    39721 acgtgttccg gcaatcgggt agatctcaat ctggcggctg gtggcgtcat acttgagcga
    39781 actttccggc gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg
    39841 gctgggatta ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga
    39901 gaaacggcga gatggcacca cctggaaaat ttctccggcg cgaatcgctt tttgcaacaa
    39961 acgcactacg ccaccgaact cttcatcgct ctggttacat tcacaacgca tatgcggcac
    40021 ggaaaccacc ggcagcggtg gcgcggtttc ggtcagttgc tggcgaagat cgttcaggcg
    40081 agcagtgaga cgttgttttt cttcttcatt cggagcaaaa aggctggcct gaatgcgggt
    40141 gctttttttc tgatggtcaa tcaccatcag cgtttcagcg agataaaaac agaaatcagg
    40201 gcagttattt tccgctgaca gttgcggtaa atcttcaaac cccgccacaa ggtcataaga
    40261 gaacaggccg ccgaagaaca tggcttctcg ttcttccttc ggtacattca acagattctg
    40321 caataagcgg aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag
    40381 tggactaaca ggggggaagc tcagcacacg gcagtttggt aattgttcat tttccacacc
    40441 cgcaggcagg gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg aaagggactg
    40501 gattgtgaca gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca gcagcaggct
    40561 ttttaaatca tctttgctgt cgatatctgc ggattccagc agcagcgttg ccggacgatt
    40621 cccacacaac tggtgaaaaa gcgcggtggg attgtcgtga taagcgcctt cgcaggttag
    40681 cagttcgaga gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag
    40741 cccgctcatt aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt
    40801 ttcaggaagt gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt
    40861 ttacgtgaac ttgcgtacta gttaactagt tcgatgatta attgtcaaca gctcatttca
    40921 gaatatttgc cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga atgggagtgc
    40981 gccttgagcg acacgaatta cgcagtgatt tacgacctgc acagccatac cacagcttcc
    41041 gatggctgcc tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc
    41101 ctggcgatca ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca
    41161 cgttcgggac tggcactgaa tcttattccc ggcgtggaaa tatccacggt ctgggaaaat
    41221 catgaaattc atattgtcgg gctgaatatt gatattattc atccgctaat gtgtgagttc
    41281 ctcgcgcagc agacagaacg gcgcaatcag cgggcgcagc aaattgccga aagacttgaa
    41341 aaagcgcaaa tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg
    41401 acgcgcggtc attttgcgcg tttccttgtt gagtgcggca aagccagttc aatggcggat
    41461 gtctttaaaa agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca
    41521 atagaacaag ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct
    41581 gggcggtaca atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac
    41641 gacggcgacg cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg tacccagctg
    41701 gcgacgcttg cccgccagca ccatttatgg gcatcacaag gatctgattt tcatcagcca
    41761 tgcccgtgga tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg
    41821 cagctatggg aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata
    41881 ttcatcctga taacccgcag caacgtctga tcaaccaggc ggtggagatc gtgcgtaaag
    41941 gcggggtgat tgtttatcca accgattccg gctatgcgct cggctgtaaa attgaagata
    42001 aaaacgcgat ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc
    42061 tgatgtgtcg cgatctttct gaactctcga cctattcatt tgtcgataac gttgcgtttc
    42121 gtttaatgaa aaacaacacg ccgggcaact ataccttcat cctgaagggg acgaaggagg
    42181 tgccacgccg tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc
    42241 cgattgcgca ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc
    42301 tgccaggcag cgaatttacc gaatccgatc ccgaagaaat taaagaccgt ctggaaaaac
    42361 aggtagattt gattattcac ggcggctatc tcgggcagaa accgacaacg gttattgatc
    42421 ttaccgatga tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat
    42481 aacaaattgc gttccacgga tggaagacta tggcagggaa gtttcgctgc attttgctat
    42541 tgatagttgg gctttttttc tcttcgttaa gttatgcgaa aaacacggag atgccttctt
    42601 atgaagaagg gatctcgctc tttgatgttg aagccactct gcaaccggat ggggtgctcg
    42661 acatcaaaga gaacattcat tttcaggcgc gaaatcagca gattaagcac ggattttatc
    42721 gtgatttacc acgactatgg atgcagcctg atggggacgc tgcactgctg aactatcata
    42781 ttgttggcgt cacccgtgat ggtattcctg aaccctggca tcttgactgg catatcgggt
    42841 taatgagtat tgtcgtgggc gataaacaac gtttcttgcc tcaaggcgac tatcattatc
    42901 aaattcatta tcaggttaaa aatgctttcc tgcgtgaggg ggattctgat ctgctaattt
    42961 ggaacgtgac cggtaaccac tggccgtttg aaatttataa gacccgtttt tctctccagt
    43021 tctctaatat tgcgggtaat ccatttagcg aaatcgatct ctttaccgga gaagagggcg
    43081 acacatatcg taatggccgc atccttgagg acggaagaat tgaatcccgc gatccgtttt
    43141 atcgtgaaga tttcacggta ctctaccgtt ggcctcactc gttacttagc aatgccccgg
    43201 ctccacaaac gacgaatatt ttcagccatc ttcttttacc ctccacgtca tcgttgttaa
    43261 tttggtttcc gtgtctattc ctggtttgtg gatggttata tctctggaag cgcaggccgc
    43321 aatttacgcc ggtagatgtg attgaaaccg atgtcattcc gccagattac acgctcggca
    43381 tgttacgtct cgatgcgaag ctggtttacg acgataaagg tttttgtgcc gatatcgtaa
    43441 atctgatagt gaaaggaaaa attcatctgg aagatcagtc tgacaagaac cagcaaaccc
    43501 tgattcgtgt taatgaaggc gtgaccagaa ataatgcggt attactgccc gcagagcagt
    43561 tattactgga agcgttattt cgtaaaggcg ataaggtcgt tcttacgggg agacgcaaca
    43621 gagtcttacg cagggcattt ttacggatgc agaaatttta tctgccgcgt aaaaagtctt
    43681 cgttttaccg gcctgatacg tttttgcaat ggggtggact ggcaatattg gcggtcattc
    43741 tctacggtaa cctgagtcct gtaggctggg caggaatgag tctggtgggc gatatgttta
    43801 ttatgatctg ctggcttctt ccttttttat tttgttccct tgagcttttg tttacccgcg
    43861 atgatgacaa gccttgcgtt aatcgtgtaa tcatcacttt gtttttaccg ctgatttgtt
    43921 caggcgtggc cttttattct ctctatatca atgtcggaga tgtattcttt tactggtata
    43981 tgccagcggg gtattttagc gctgttttcc tgaccggtta tctcactggt atggggtata
    44041 tttttctgcc aaagtttacc caaactgggc agcaacgtta tgcccacggt gaagctatcg
    44101 ttaactatct tgcgcgtaaa gaggcggcaa cacacagtgg gcggcggcgg aaaggggaaa
    44161 cacggaaact ggattacgcg ttgctaggtt gggcagtctc agcaaacctt ggaagagaat
    44221 gggttgcacg tatcacccca tcactcacag cggctgttag cgccccggaa attgcccgta
    44281 gtggcgtttt gttctcatta cagatgcacc tgagtctggg ggccaatacc agtttgttgg
    44341 ggcgaagtta ttccggtggt ggtgctggcg
//
LOCUS       DAZGCQ010000032        43754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000032 DAZGCQ010000000
VERSION     DAZGCQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.032"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(74..1327)
                     /gene="amiC"
                     /locus_tag="ACHK07_001896"
     CDS             complement(74..1327)
                     /gene="amiC"
                     /locus_tag="ACHK07_001896"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO8102232.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1559..2890
                     /gene="argA"
                     /locus_tag="ACHK07_001897"
     CDS             1559..2890
                     /gene="argA"
                     /locus_tag="ACHK07_001897"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO8102233.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2952..4778)
                     /gene="recD"
                     /locus_tag="ACHK07_001898"
     CDS             complement(2952..4778)
                     /gene="recD"
                     /locus_tag="ACHK07_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO8102234.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4778..8320)
                     /gene="recB"
                     /locus_tag="ACHK07_001899"
     CDS             complement(4778..8320)
                     /gene="recB"
                     /locus_tag="ACHK07_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO8102235.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESRLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLSDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYSLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAELITLMDEMFAGMTLEEA"
     gene            complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="ACHK07_001900"
     CDS             complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="ACHK07_001900"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO8102236.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAASIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11377..14745)
                     /gene="recC"
                     /locus_tag="ACHK07_001901"
     CDS             complement(11377..14745)
                     /gene="recC"
                     /locus_tag="ACHK07_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO8102237.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14758..15081)
                     /gene="ppdC"
                     /locus_tag="ACHK07_001902"
     CDS             complement(14758..15081)
                     /gene="ppdC"
                     /locus_tag="ACHK07_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO8102238.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(15066..15473)
                     /gene="ygdB"
                     /locus_tag="ACHK07_001903"
     CDS             complement(15066..15473)
                     /gene="ygdB"
                     /locus_tag="ACHK07_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO8102239.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMYSWQTQPAVQCLQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRAGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15470..16033)
                     /gene="ppdB"
                     /locus_tag="ACHK07_001904"
     CDS             complement(15470..16033)
                     /gene="ppdB"
                     /locus_tag="ACHK07_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8102240.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(16024..16494)
                     /gene="ppdA"
                     /locus_tag="ACHK07_001905"
     CDS             complement(16024..16494)
                     /gene="ppdA"
                     /locus_tag="ACHK07_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8102241.1"
                     /translation="MKTQRGYTLIETLVAMLILVTLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="ACHK07_001906"
     CDS             complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="ACHK07_001906"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO8102242.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="ACHK07_001907"
     CDS             complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="ACHK07_001907"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO8102243.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="ACHK07_001908"
     CDS             complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="ACHK07_001908"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8102244.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="ACHK07_001909"
     CDS             complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="ACHK07_001909"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO8102245.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21648..21794)
                     /gene="ygdT"
                     /locus_tag="ACHK07_001910"
     CDS             complement(21648..21794)
                     /gene="ygdT"
                     /locus_tag="ACHK07_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO8102246.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFGGSVLNLVAHSVDK
                     YGIG"
     gene            21980..22669
                     /gene="mutH"
                     /locus_tag="ACHK07_001911"
     CDS             21980..22669
                     /gene="mutH"
                     /locus_tag="ACHK07_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO8102247.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22738..23451
                     /gene="ygdQ"
                     /locus_tag="ACHK07_001912"
     CDS             22738..23451
                     /gene="ygdQ"
                     /locus_tag="ACHK07_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8102248.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23589..23807
                     /gene="ygdR"
                     /locus_tag="ACHK07_001913"
     CDS             23589..23807
                     /gene="ygdR"
                     /locus_tag="ACHK07_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO8102249.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23915..24955
                     /gene="tas"
                     /locus_tag="ACHK07_001914"
     CDS             23915..24955
                     /gene="tas"
                     /locus_tag="ACHK07_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO8102250.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24987..26180)
                     /gene="lplT"
                     /locus_tag="ACHK07_001915"
     CDS             complement(24987..26180)
                     /gene="lplT"
                     /locus_tag="ACHK07_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO8102251.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26173..28332)
                     /gene="aas"
                     /locus_tag="ACHK07_001916"
     CDS             complement(26173..28332)
                     /gene="aas"
                     /locus_tag="ACHK07_001916"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO8102252.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28918..29949
                     /gene="galR"
                     /locus_tag="ACHK07_001917"
     CDS             28918..29949
                     /gene="galR"
                     /locus_tag="ACHK07_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO8102253.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29956..31218)
                     /gene="lysA"
                     /locus_tag="ACHK07_001918"
     CDS             complement(29956..31218)
                     /gene="lysA"
                     /locus_tag="ACHK07_001918"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO8102254.1"
                     /translation="MPHSLFSTDTALTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31340..32275
                     /gene="lysR"
                     /locus_tag="ACHK07_001919"
     CDS             31340..32275
                     /gene="lysR"
                     /locus_tag="ACHK07_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO8102255.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32262..32954)
                     /gene="ygeA"
                     /locus_tag="ACHK07_001920"
     CDS             complement(32262..32954)
                     /gene="ygeA"
                     /locus_tag="ACHK07_001920"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO8102256.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEELSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33083..34501)
                     /gene="araE"
                     /locus_tag="ACHK07_001921"
     CDS             complement(33083..34501)
                     /gene="araE"
                     /locus_tag="ACHK07_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO8102257.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34816..35577)
                     /gene="kduD"
                     /locus_tag="ACHK07_001922"
     CDS             complement(34816..35577)
                     /gene="kduD"
                     /locus_tag="ACHK07_001922"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO8102258.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35607..36443)
                     /gene="kduI"
                     /locus_tag="ACHK07_001923"
     CDS             complement(35607..36443)
                     /gene="kduI"
                     /locus_tag="ACHK07_001923"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO8102259.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVTDEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCVPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(36730..37911)
                     /gene="yqeF"
                     /locus_tag="ACHK07_001924"
     CDS             complement(36730..37911)
                     /gene="yqeF"
                     /locus_tag="ACHK07_001924"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8102260.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38166..39395
                     /gene="yqeG"
                     /locus_tag="ACHK07_001925"
     CDS             38166..39395
                     /gene="yqeG"
                     /locus_tag="ACHK07_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8102261.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            39855..40487
                     /gene="yqeH"
                     /locus_tag="ACHK07_001926"
     CDS             39855..40487
                     /gene="yqeH"
                     /locus_tag="ACHK07_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8102262.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            40821..41630
                     /gene="yqeI"
                     /locus_tag="ACHK07_001927"
     CDS             40821..41630
                     /gene="yqeI"
                     /locus_tag="ACHK07_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8102263.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            41623..42105
                     /gene="yqeJ"
                     /locus_tag="ACHK07_001928"
     CDS             41623..42105
                     /gene="yqeJ"
                     /locus_tag="ACHK07_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO8102264.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTSNWVIF
                     NDQGRANLTIDFMYNQKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTS
                     KKVNKFDIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(42138..42218)
                     /locus_tag="ACHK07_001929"
     CDS             complement(42138..42218)
                     /locus_tag="ACHK07_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102265.1"
                     /translation="MKDVDQNFDALYWHILQSYLILLFYD"
     gene            complement(42254..42679)
                     /gene="yqeK"
                     /locus_tag="ACHK07_001930"
     CDS             complement(42254..42679)
                     /gene="yqeK"
                     /locus_tag="ACHK07_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO8102266.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLILSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            43109..43600
                     /locus_tag="ACHK07_001931"
     CDS             43109..43600
                     /locus_tag="ACHK07_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102267.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKFVNSVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMCIYKLPITDDLMSMATNELIKLT
                     KIENNLDLNKFATICTDVLSPLVTRHNKQKNQNDILPFAKIPFLIFIEKKEIDNMNPG
                     HAH"
BASE COUNT        11277 a        11452 c        10336 g        10689 t
ORIGIN      
        1 attattgaac accctaacgg gtgttttttt gtttctggtc tcccataaaa aagcgccatt
       61 cagcgccttt ttatcatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa
      121 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      181 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      241 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg attttaccga
      301 gcttattcag cactgcttta ccaaacttca ggctgtcggc aatggtcagc gactgcacca
      361 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
      421 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgca cctttggttg
      481 agagcgcaaa cacagaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag
      541 agacaaacag gtcagcacgc tgtttctgag cttttgctac gcgcacttgc aacggaatga
      601 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc
      661 gcagacggcg agctatttgc aataccacgt ctttttcgcg cgttttgtat ttccccaccg
      721 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
      781 ctgctttacc tggttgtgga ccactttgcg ctggtggcac ctgcttttcg aggtcgcctt
      841 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
      901 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
      961 gttttacgtt ttgctttaat tcaaaaacca tccgtacggt ttgcgggtca aattgcccga
     1021 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
     1081 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttccgggtta ctcaacgcga
     1141 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1201 gccagacgcg caccgccacg acctggctga ccgcagccag actgacctga cttacgctca
     1261 atagccacat ggcacccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1321 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
     1381 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
     1441 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
     1501 tgcgaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaaga ggtgtgccgt
     1561 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     1621 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     1681 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     1741 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
     1801 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     1861 gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct
     1921 acagggcgcg catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt
     1981 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat
     2041 ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
     2101 tggcgagagc tttaacctga cctcggaaga gattgccact caactggcca tcaaactgaa
     2161 agctgagaag atgattggtt tttgctcttc acagggcgtc actaatgacg acggtgatat
     2221 tgtctcagaa cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg
     2281 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2341 cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     2401 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     2461 aacaatcaac gatatcggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     2521 tctggtacgc cgttctcgcg agcagctgga gatggaaatc gataaattca ccattattca
     2581 gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
     2641 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagc tcatcacggg gtgaagttct
     2701 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     2761 cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata ttgatttatt
     2821 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     2881 tttagggtaa gcgatgaaaa tcgccggatg cggcaggcgt aacactcgtg cgtcgcatcc
     2941 ggcaattacg tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3001 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc
     3061 gcgcgggtca ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt
     3121 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3181 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3241 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     3301 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag
     3361 tgcggattac gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt
     3421 ccagccacgc caaatggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc
     3481 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc
     3541 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg
     3601 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
     3661 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     3721 cagagactgt cgcgcaaaga tgccgcttct gtgccagttc ctgccggaac gtgagtcccc
     3781 gtcaggcggc ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag
     3841 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     3901 atcactcgcg catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
     3961 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4021 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
     4081 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
     4141 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4201 tctgccattt gaattaacgc tgccagcaac ttcgctacgg tggtcgtttt accggtgcca
     4261 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgccgccgc aactttttgc
     4321 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag taaagcttca
     4381 tcaacctcaa tggcatgatt cacttcgttg aaaaagcgtg ccactgtgcg ctcgttacac
     4441 cacatgcgat tcaaataaag acgatcacca cagaggatca tcggcgtggg ttcatctccc
     4501 cggctgaccg cttgtgaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
     4561 ctgacacagg tcgccaacag cggatgcgac gcctcattat tttccagtcg tgaaagcggc
     4621 aaacaaacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
     4681 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
     4741 tgctccacag cttccagtaa ttgcttctgc aatttcatta cgcctcctcc agggtcatac
     4801 cggcaaacat ctcatccatc agggtaatca actcggcgtt ggggcgagtt gtgtaaatcc
     4861 cctgttgcgg atgttcttta tcaacgccac gcaggaacag ataaataacg ccgccaaagt
     4921 ggcgctcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat
     4981 aaagctgata ttgcagatca tagcggtgtg cctgcattgc cgctgccata gcctgttggg
     5041 tgtaagccga actgtcttca cccaaccagt tggatttata gtcgagcagg taataacgcc
     5101 cttcgtggcg gaacaccagg tcgataaagc cttttaacat gccacgtacc tgcatgaact
     5161 ccagcggcgg gcagcctgcg gatagcgggt caaactggcg gattaacgta tcaagctgac
     5221 tggcgataag cggttcacta atcggcagat aaaactccat ctccacctgt ttattgcggg
     5281 cggaaagttg actcaggctt acgccggttt cattgagagg tgcctggagg acagccgtga
     5341 tccactcggt caataccggt tcccactgcg attcaaagcc gccgagttcc agtttttccc
     5401 gcacccagtt cgggtcaacc ggctgggtaa aatccaggtc ttcaaacaaa ctgtgcaaga
     5461 acgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     5521 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     5581 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     5641 gtaacgtctt cgcattcagc tctgctgtag aaacatcatt aacctgccag ggttggttat
     5701 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
     5761 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
     5821 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
     5881 tacaatgcca aaccgaacgc gtcagcgcca cataaagcaa acgcagatct tccgccagac
     5941 gttcggcctc cgcgagatcg acactttctg gcgcagcgtt aagatccaga acagcctcaa
     6001 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6061 accagacaag cgaatattcc agcccttttg atttatggat cgtgacaatc tgcaccagat
     6121 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6181 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta
     6241 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     6301 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     6361 tgcgccagat ttgccgataa ccatcgaact cttcgaccac cgcgtcccac gcatgttcgt
     6421 cattattcag cgtttcgata tccagcgcgt tcagccccat cattgacgtt gccagcgcac
     6481 tacgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct
     6541 gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca
     6601 gcaacgttaa ggcatcgcgc acctgggcag cctcctggcg gctgcgcacc agcacactga
     6661 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
     6721 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     6781 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     6841 cacctttaaa tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
     6901 gtatttcgcg aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acgctgttca
     6961 ccattcctgg tgcggaacgc cagttggtgt ctaaagtgta gtgggcgtga acttcgctac
     7021 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
     7081 cgccaattag caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt
     7141 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7201 tcgccgctgc caacacctca ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
     7261 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     7321 cagccaatgc gcgggtgatc accagatcgc ggatcgacaa tggttctgca agcagttgat
     7381 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta
     7441 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
     7501 ctgcccaggc gctgatcttg tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     7561 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     7621 gttttaccgt atcaatacgc gccacaattt gcgcgtgacg ggaagccagc gtttcatcat
     7681 cgggcggcgg tgctttgata accggcgctt cgccttgcag ataacgatta atatcgctca
     7741 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
     7801 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagccga gactcatctt
     7861 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
     7921 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
     7981 accactgcgc ggcttgcgct ttatcgtcga tctcttccag cagacgttcg tacagtggat
     8041 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8101 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca
     8161 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg
     8221 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     8281 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     8341 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     8401 tcggctttcc cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg
     8461 accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc
     8521 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     8581 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
     8641 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
     8701 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     8761 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
     8821 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     8881 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
     8941 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
     9001 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
     9061 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
     9121 aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
     9181 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9241 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
     9301 agctgatctt ccgtagcggt atagctaaag tatccctcga gcaatgcctg gaacagctgc
     9361 ggcagacgct gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa
     9421 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
     9481 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
     9541 aaaatcaggc tgacatcagc tttgggttcg ctggcaaaat aacggcttgg cgcatacacc
     9601 acgcgcagat tcgactcatc aacaatcagc tctggatggt catatttctt ctctgactta
     9661 atcagcgaga aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatactg
     9721 gcagcttttt gctgccagtc agcgaaagtt tgttcgctga ttttatcgac ctgatacggc
     9781 gcatcgacaa aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc
     9841 gcattctgcg gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg
     9901 gcaatattga ctgcatccag cgtatgctca accggaacgc gaatcatggt atctgccagc
     9961 cattcgacgt aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca
    10021 ttcgccagtt catcgaagta ttgtttatcg atcccttttt cacgtaacaa attgaggtag
    10081 ctaaaaatag ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca
    10141 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    10201 tcaactaacc cctgcttttg cagccagtca gacagtgtac ctgggctgcg attaccaatc
    10261 agataggtaa tcaattcatc cgttttactg cggaacttcg ctgagttgtt atcgatgcga
    10321 aactcaacgc gcagaacttt acgcggcagc gctgggacgt aatgaatgat aatgcccttt
    10381 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    10441 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt actgtaaata
    10501 accgccttca tcaaattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    10561 accggattac caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    10621 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    10681 cgcgccatgg ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg
    10741 agcaagggtt ctgcaatagc atcggccagg cggtctaccg caccgggcaa ggcgtcgttc
    10801 tcaacttcca gatagaaagc cgtgcgatac ggtgccgtgc tggcattgtg actaccgccg
    10861 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    10921 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    10981 acgggcacca ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    11041 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11101 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccaaagggca
    11161 actaacaaca ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa
    11221 ctttattttc tggccagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg
    11281 tgctgcacga gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    11341 agtaagcgga tagattgcgc aatttttata cagcgctcat gactgattaa agcgaaacag
    11401 cggtaacagg aaacgttgcg actgttcaac gattgcctcc attgtctctg gtgttaattg
    11461 ccgccagagc ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    11521 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    11581 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    11641 caatggcgcg gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    11701 ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact
    11761 ttcaccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    11821 cgccacactt aataaagagg gacgccagcg caacaagcca tccggctgca cctgcggcaa
    11881 ccaaccagtt atctgcacac cgttgcaagc gagatcaatt tccatactct gccccggctg
    11941 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa
    12001 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg
    12061 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    12121 aagtccttcc agaataaatg gctcggtgtc ggggatttcg ctgtcttcag tacggaagtt
    12181 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12241 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    12301 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    12361 ctgatagttt tgtggatcaa acggcattcg ggtatggagg caagtaagat gcgcttttac
    12421 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    12481 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    12541 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    12601 cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    12661 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    12721 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggcccggc
    12781 gagaaaacgc tggctgatac gttcttgatc caggcgctgt gccagttcat cacgcaatag
    12841 tgacagcggc accgcatcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    12901 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt
    12961 gagcatatcg cgacaaaccg gcaaccactc ttccagcgga cgctcctgtg ccagcccgcg
    13021 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13081 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13141 gtagcccaac aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    13201 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13261 ctgacgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    13321 atccagtaat gccagcacgt cttccgacac aaagcggctg tctggcaacg ataacaggct
    13381 gataaacgct tcaagtacag gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg
    13441 caggtaacga tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc
    13501 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat
    13561 cgccagcagg cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    13621 ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    13681 gatgttcaca ccagcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg
    13741 cagcaggtta tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc
    13801 agaaaggaga taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc
    13861 gacatcctgt tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cgcgaaatag
    13921 cggcaattcg cgatcttcaa aactgtgtcg acgctgacgg gtcagcagtt tcgccagata
    13981 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14041 ttcaatatgt ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat
    14101 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14161 ttcgataaag cgctgataga gattcgcgcg gtgccagcgc ggttgcccga gttcatgggt
    14221 atattccacc agagccttcc acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc
    14281 aaccaaatgt cctgtttccc attgtgccag ccactccggg cgatagacca gatactggtc
    14341 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    14401 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    14461 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggatct ccggtaacac
    14521 ccggacgaac atatcccaga taaagctcgc aggcagcgga aaatcaatgt ttgccgcaat
    14581 accaaacttt tgcgacaggg tcatttgcag ccactgtgcc ataccggtac tttgcaccag
    14641 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    14701 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    14761 ctgacgattc gggcaatgca ggcgggtcat ctcgcctgct ctgcccccgg gtgaaactag
    14821 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    14881 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg
    14941 gtactggttt ctgctggcaa gactgttcat taatgttcgc tggaaacccg ataacgcagt
    15001 gacaatcatc accatcaaca ccatcgccaa cattacctcc ggcaggctaa agccttgttg
    15061 attcttcagg gaagctgaca taacgccccc tctttcagcg gacaaaaatc gctccagccg
    15121 cgtggcgaaa aaacaatttt tccatcgatg acttcacctg ctcgccacaa cgaaacgcct
    15181 tcataaccgg caatcaataa ggcttcatta tctgccaata aacgcaaaca aacccgggca
    15241 tcggttccag cgtactgcaa gcactgaact gctggctgcg tctgccagga gtacattttt
    15301 ccccacgcca gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc
    15361 acgcgagagg caaaactgcg atcctgttgg ctcattcctt gtaatagcaa gctacccaaa
    15421 actagcagca tcaggaccag agccagtgac gaaacaccct tttcgcggtt cacaggttga
    15481 atcctgtcac gctatagctg gcattcacca cagtttgcgg ttcagactta ctggcagcac
    15541 gcatattaac cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag
    15601 tgtcgataat gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg
    15661 tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcggact
    15721 cttttaccgg ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgacac
    15781 agtcaccctg tccgacaatt tccagccctt cgccggtaca gatgccatga caataacccg
    15841 ccctctggag atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagtgcca
    15901 gcttacgggt gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca
    15961 acaacaatac gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct
    16021 cttttacagg catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga
    16081 aaccaccagc caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt
    16141 attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca
    16201 gcgtggcaca aagaccaatg gtgaactgcc atgacaggta ttcgccccag caacggaact
    16261 cacaaggcac cataacgtcc cctccctgat aaaactgata ctgtggtcgc ggttatgcca
    16321 gttggcatct tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccaaag
    16381 ccgttgcgac tgctgccagt attgccagcc atagagtcca cttgcgctta gcgtgaccaa
    16441 aatcagcatc gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc
    16501 agtatggagc gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc
    16561 ggactattta cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta
    16621 atggagggta aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    16681 agatagccac cggcgcttta atacccggat gcggatcgta gccttcaatc tcaaagtctt
    16741 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    16801 gcggttcgcg gcttaattgc agatgagttt gatccatatg attgctgtac aggtgcgtat
    16861 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    16921 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    16981 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    17041 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atacggcggg
    17101 aatccgggtc gtttttcagc tggttcagta ccgtagtgat ctggtcaata tgacgaccat
    17161 ccggcgttgg ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccgttttcat
    17221 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc
    17281 cctgcagaaa ccacagcagt tcatggatga tggaacgcag gtggcaacgt ttagttgtca
    17341 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    17401 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    17461 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    17521 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    17581 actgcaccca ggcaccggta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    17641 gaaacgcgcc gtagccaatc aggaacaagc ctgagacagc tcccattggc cgcggtttac
    17701 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagt tcgtaaagct
    17761 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    17821 tttgcagcag caaaatatct tctgtacggg agcctgggaa cagcatggca aacgggaagt
    17881 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcgccaa
    17941 gaccaaacgg aatgagcggt gctataaaat cagatacctg gaagaaggaa cgtttggtgc
    18001 ggcgggcgaa gataatcatc accacaatta cgccaatcag gccgccatgg aaggacatgc
    18061 cgccgtccca gacgcgaaac agatacagcg gatcagccat aaactgcggg aagttgtaga
    18121 acagaacata cccaatacgt ccgccaagga aaacgccaag gaagcccgca tagagtaagt
    18181 tttcaacttc atttttggtc cagccgctgc ccggacgatt cgcccgacgt gttgccagcc
    18241 acattgcaaa aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    18301 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    18361 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    18421 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    18481 ttaaaagata tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc
    18541 gacgctccat aaaggctgca acctgatggc gaacttcggt cgccagttgc gcttccagac
    18601 tacgctgcgc aagattttct gcttcggcat aatcaatgcg ccgcagcagg tattttaccc
    18661 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    18721 cgcacatggg atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg
    18781 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt
    18841 tcgccacccg ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc
    18901 caacagagat gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggta
    18961 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    19021 cacgtccggc acgttcaatc aggcggcgag cttcgtcaac ttcatcgagg ctggtgacca
    19081 tcggcaacag aatattcagg ttgcccgtag cggcgttagc acgcagcatc gcccgcacct
    19141 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    19201 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    19261 gcaaggtgac gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt
    19321 cttcttccga cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga
    19381 tgccatcaat acggctgccc agtttttctt catgttccgg gcttaaacca gcattgagca
    19441 tgacttttat acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca
    19501 gacggctaag ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct
    19561 ccggatcgac cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca
    19621 ccgaaggctg aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg
    19681 cagcatggga gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca
    19741 gctcagcaag agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    19801 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    19861 aatcgccagc gcgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    19921 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    19981 attcgcgacg cagccgggtg tccgaaagca ggtgcgagta aagatcgaaa atagccgccg
    20041 tttctttttg tgcaccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt
    20101 cttccagcgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    20161 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    20221 gtgctgccgg taatgcgcgg attcgcgtct ggcgatattg cccaaacaag gcagtcaact
    20281 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    20341 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    20401 gttgaataat tggtacgcct aaaaacgcgc ggaagcgttc ttcttttacg gaggggatgt
    20461 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    20521 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    20581 gcccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    20641 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    20701 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg
    20761 tgacttaacc tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct
    20821 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    20881 atgacaccac ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg
    20941 gtgtactgct ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc
    21001 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    21061 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    21121 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttaattc
    21181 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    21241 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    21301 tacctcaata taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac
    21361 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    21421 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    21481 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga
    21541 cattttaaaa tattcattat cagtcaatgc attacatgta atttaagtaa tgaaaaagag
    21601 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    21661 tatttatcca cagaatgtgc cactaagtta agcactgaac caccaaaaac tggagtttcg
    21721 tcgcacgtca aggctgtaaa tggaaacagt agtggaggtt tttcacagtt atcccagctt
    21781 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaacccg
    21841 ttgcagtgtt gcgcaactcg attaccggca acctaaaaag caatataaat cagagaattg
    21901 aacaacgcat gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt
    21961 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactg ctctctcctc ccgaaactga
    22021 agaacagttg ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc
    22081 acttgccggg ctggttacgc cagagaattt aaaacgcgat aaaggctgga ttggcgtgtt
    22141 actggagatc tggctaggtg ccagcgcagg aagtaaacct gagcaagatt ttgctgctct
    22201 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt
    22261 cgtttgtgtt gccccgttaa cgggcaatag cggggtgacc tgggaaacca gccacgtgcg
    22321 ccacaagctc aaacgcgtgc tgtggatacc ggttgaaggc gagcgcagca tcccgctggc
    22381 gcagcgtcgc gttggatcac cgttgctgtg gagcccgaat gaagaggaag accggcagct
    22441 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcaggttg agcggattac
    22501 cgctcgtcac ggggaatatt tacagatacg accgaaagca gcgaatgcga aagcgcttac
    22561 cgaagccatt ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa
    22621 gaaaaatttc accagtgcgc tgctggcccg tcattttctg atccagtagc catcgctttg
    22681 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    22741 ttatttgcat ggataaccga tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg
    22801 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagcttccc
    22861 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg
    22921 gcgctgctgg catcaatcgc ctgggttacg cgcctgacaa atccgctttt tacaatattc
    22981 agtcaggaaa tttctgcccg tgacttgatt ctgcttctgg gtggcttgtt ccttatctgg
    23041 aaagccagca aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc
    23101 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    23161 gattcagtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggcagccgtg
    23221 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc
    23281 catccttcgg taaaaatgct ggctctctct ttcctgattc tggtgggctt taccctgatt
    23341 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    23401 attgcggttg aaagcctcaa cctgattcgc aacaaaaaga atccgctctg atactccgta
    23461 cgctctcctg cgggagggcg tactcactcc tgttaccctc acagtttaag attttcctgc
    23521 tttcaagata tataacgtcg gtttataaac agactattat cataggtggg ccaaatgagg
    23581 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    23641 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    23701 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    23761 cgatgcaaat taaccgtgat gatgtttcgc aaataattga acgttaacaa ataaggtcag
    23821 catccggctg gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata
    23881 ttccttatgt cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag
    23941 ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg tttggtgaac agaacagcga
    24001 agccgacgcc cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt
    24061 tgccgaaatg tacccagtac ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt
    24121 cggcaactgg ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag
    24181 cggaccgtcg cgcaataatg acaagggcat ccgcccggat caggcgctgg atcggaagaa
    24241 tatccgcgaa gcgctgcatg acagcctcaa gcgtctgcag actgattacc tcgatcttta
    24301 tcaggtgcac tggccgcagc gcccaaccaa ctgcttcggc aaactcggtt atagctggac
    24361 agattctgcg cctgcggttt cgctgctgga tacgctggac gcactggcag agtaccaacg
    24421 agcgggaaaa attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta
    24481 cctgcatctg gcggacaaac acgatctgcc gcgtattgtc accattcaga acccttacag
    24541 tctgttaaac cgcagttttg aagtaggtct ggcagaagtc agccagtatg aaggggtcga
    24601 actgctggcc tattcgtgcc tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc
    24661 aaaacccgct ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca
    24721 aacgcaaaaa gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc tggatcctgc
    24781 tcagatggcg ctcgcttttg tacgtcgtca accgtttgtt gccagcactc tgctgggcgc
    24841 aaccacgatg gagcagctga aaactaacgt cgaaagtttg catctggagt tgagcgaaga
    24901 cgtattagct gaaattgaag cggtgcatca ggtttatacg tatcctgcac cataagatta
    24961 acgccggtta aaaccggcgt taaatattaa tgacggcgct gccagatcca cagcgccgtt
    25021 attgccagcg caaatagcgc accgaagcca atgccaatgg gcacgaccgg gatgcctacc
    25081 attaccgcca gcgagtaaat gcccagcatc aacaacatgg cgctattttc gccaaggttt
    25141 tgtactgcaa tcgcattccc cgccccgacg ctttttttac cccgctcctg tagcaacgca
    25201 ttgagcggaa cgacaaaaaa gccccccagc acgccaatca gcatcaacaa ggcataggct
    25261 ggcagcagct cgtgttgcag ggagaaaatc agtaccacca cgccaatcaa aatcccggct
    25321 ggcatacagc gtgacacggt ttccagcgta accagcttcg ctgccgcacc tgcgccaacc
    25381 acgataccaa tcgctaccat cgcgttgaga taggtcggcg tagcgttatc ggtaatgccc
    25441 agcgccaccg gtacccacag caccaacagg aaacgcagcg tgacacccgc tccccagaat
    25501 aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc accatagcga ggtgcaggca
    25561 ttcaggaaac tgcgggtcat gttgatgaga ttccaggact gccccggacg cgccgccgcc
    25621 agtttgggaa tgtagatatt ggcaacgacc gcaccaccgt aggccagtgc gcatgcggcc
    25681 agggcgacca ggacatgcca gtcagccagc acaccaccgg ctacggaacc gagcaaaatc
    25741 gccgctatgg tagaagcttc cattaaaccg ttagctttta ctaacttact acccgtggtt
    25801 aattcgccga gaataccgta tttcgccggt gaataggctg cagcaccaac acccaccagc
    25861 gtatagccga gaaacggatt gataccaaag cagatactgg ctgcgcccag cagcttcagg
    25921 ccgttggcaa acatcatcac ccggcctttg gcgaagctat ccgccacctg cccgacaaac
    25981 ggcgcaaaaa gaatgtaagc acctacaaac accatttgca ggatgggctg gctccactcc
    26041 ggatagaact gcgctttcag taacgccaga gtggcaaaca gtagtgcatt atcgccaaac
    26101 gcagagagaa actgcgccac gataaccgct ttcatcccct tcgaccacaa cgaagtatta
    26161 gtgtgcactg actcactcat cgtgttgttc cgcttcgtct acccagcttt tcaacgtgac
    26221 aaagtcaggt ttgccgctgc caagtaatgg catctgtttc agataacgaa tatcacgcgg
    26281 tacagcaagc tccggcacac cgtgctcgcg ggcatactgt tgcaacttat cgcgcgtcag
    26341 ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg ctggcatcgc tcttaattgc
    26401 agtggcatgg actttatctg gcgaaacacc aagtgccagt tgttccacca tttccagcga
    26461 caccatttcg cctgcaattt tggcaaagcg ttttgcgcga ccctgaatct gcacaaagcc
    26521 ctgctcgtca aaacgcacga tatcgccagt gtcgtaccag ccgcgctcca tttcgccgcg
    26581 cacattctcg gcggtgggca cttccagtac acctggcttc tccacccgca gatagccgtt
    26641 cattatgttc ggccctttca gttgcaggcg tccgccctct tcgataccag gaaccgacaa
    26701 caggcgcgca tccattcctg gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg
    26761 tacgttgata gaaacgacag gcgcgcactc ggttacacca tagccttcaa ggatgcgcag
    26821 gccaaattta tcctgccaaa gctgtttggt actttcttgt agtttttctg cgcctgccac
    26881 cacatagcgt agacgataga aatcatacgg gttggcgaaa cgcgcgtagt gaccgaggaa
    26941 agtggaggta ccgaacaaca cggtgcaact gcggtcatac accaactccg gcacaatgcg
    27001 gtaatgcagc gggcttggat aaaggaacac ttctgcacct gtgagcagtg gcgtaaacag
    27061 gcctaccgtc agcccaaagg agtgaaacag cggtaacgcc gacataaagc gatcgttggt
    27121 ggtgaagtcg gcaatcgttt taatctgctc gacattcgcc agaatgcttt tatggctatg
    27181 gacgacgcct ttcggatggc cttcagaacc ggaggtaaaa aggatcagcg cctcttcttc
    27241 cggctgctgt ttaacctgtg ccagacgcgg catcagcaaa tgagcgaaga tccatacttt
    27301 gtcggcagtg gtgacatctg cttttaaatc ttccagatag acccagcgca cctgagtaag
    27361 ttgctccggc agatgccaga gtttgccttt atcgagaaac tggcgggaag tgaagatggt
    27421 tttgatttca gccgccgtaa tggcgctggt cagccctttt accccagcgg tgtagttcat
    27481 cattgcggga atgcggcgac gggcgatggc cccaaaaatc actgccgcac tgatgcctgc
    27541 attgggcagc attaagccga tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc
    27601 aacaaacagc gttttagtaa gcaatttgcg ataggagtct ggggtaaagt tgacgtcttc
    27661 gacacatttc ttcccggctc cgaagcggta cattgcactc agtaaagatt cgtacagcgt
    27721 ttcacgcggg cgcaccgcca tacgcgcttc catcattatt tgatgcagca tttcgccagc
    27781 gattttgcga cggtcacggg cacgcggcgc atccggcatc tccacctgcg ttggtggcaa
    27841 aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc agacctttca ggcggctgaa
    27901 gtgcgtaagt tccgcccctt caatacgcac aggaataacc gttgcaccag acttcgccgc
    27961 gacaaaaccc gcgccatcgt agattttcat cagcgagcct gtggtggtga tgcgtccttc
    28021 agggaaaatc accactggtc ggccctgttc caccagacgt accagatgtt taatcgccat
    28081 aggttgcgtc gggtcgagag gaacaaagtc gataaatgat ttcagccaac gcatatacca
    28141 ctgttgactt attgaggtgt aaacggcaaa taccggacgt acgggtaaaa acaatgccag
    28201 cagtatgcca tcaataaaag agacgtgatt aggcgtgatt agaacgcgct cgcccttcaa
    28261 tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa
    28321 gctaaaaagc attccaactc cctttgctct gattcagtaa aagcgaatgg agggagatta
    28381 cacgagataa agaacgcgag cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc
    28441 gcaggttggt gcaagagaca gggtacgaag agcgtaccga ataatctcac caatcaatac
    28501 ctctgggatc ttgattgtgg tctgcacgac gactcttcgc cagcgagaaa acgcaaagga
    28561 atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg
    28621 caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg
    28681 acttcaccta tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca
    28741 gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg
    28801 tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc ccttgaacca
    28861 acgggcgttt tccgtaacac tgaaagaatg taagcgttta cccactaagg tattttcatg
    28921 gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc
    28981 attaataatt cacccaaagc cagcgaagcg tcccggctgg ctgtgcatag tgcaatggag
    29041 tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg
    29101 gtcggtctgg tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc
    29161 gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta ccacaacgaa
    29221 caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc gttggtcgtc
    29281 catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg
    29341 gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct tgacgatcgt
    29401 tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg cattggttat
    29461 ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc
    29521 cttgctgaaa gtggtattcc ggccaatgat cggctggtga catttggcga accagacgaa
    29581 agcggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt cactgcggta
    29641 gcctgttata acgattcaat ggcggcgggt gcgatgggcg ttctcaatga taatggtatt
    29701 gatgtaccag gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg
    29761 cgaccgcgcc tgaccaccgt gcgttaccca atcgtgacga tggcgaccca ggctgccgaa
    29821 ctggctttgg cgctggcgga taatcgccct ctcccggaaa ttactaatgt atttagcccg
    29881 acgctggtac gtcgccattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc
    29941 agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg tctggcgacg
    30001 gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg gacggctgtt
    30061 gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc
    30121 cgccttcact tccggcaagg cgcgggtttc gacatttccc ccttcctgct gggtaaagac
    30181 atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg cgtgttccag
    30241 agaacgacca tcagctgcca gagcactgat atggtggtaa ctaccgtaca ttgctgggcg
    30301 catcagatcg ttgaacccgg catcaacaag cacaaagtgg cggctaccca tttgtttgac
    30361 gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac ccggttcaat
    30421 ttccagtttc acagggtggc ccaaatggcg ggcaatttgc tcacgcgcgg cattccacag
    30481 accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa cagaaagccc
    30541 accgcccgca gaaatagcct gtaaatcctg accgaattcg aggacctgac gcaccatagc
    30601 accacacacc tgttccagat gggcataatc aacgccagaa ccaatgtgca tgtgaatgcc
    30661 gaccagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca
    30721 gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc cgtgaccaaa
    30781 ccccgggtta acgcgcagcc atacccgatg cccaggcgaa acctggccca gttggtcgag
    30841 catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc
    30901 ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg gattgtaacc
    30961 cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca cgccctgctc
    31021 acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc gcaccacatc
    31081 aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg
    31141 gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagagcgg tatcggtgct
    31201 gaacagtgaa tgtggcatag caaactccag ataagtgctt ttttatgatt acgccacatc
    31261 ataaaaagaa taaaaaatat cgatttatgt cgagtctatg caaaaatgat atggattacc
    31321 ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc
    31381 ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct cacagccaac
    31441 cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcgt
    31501 acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc
    31561 ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt ttcgccaggg
    31621 agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc agctcctgca
    31681 accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg aatcaccgct
    31741 acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga cactccatac
    31801 gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc
    31861 gggtcatcca ctggcggtaa aaaaggtatt aacgccggat gattttcagg gtgagaacta
    31921 catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat ttactgaaca
    31981 tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtgt gcgcaatggt
    32041 acgggcgggg gtaggtgttt cggtggttaa cccgctcacc gcactggatt atgcggcaag
    32101 cggtttagtg gtgcggcggt tcagtattgc ggttccgttc accgtcagcc tgatccgccc
    32161 cctgcatcgc ccgtcatcag cgctggtgca ggcgtttagt gagcatttac aagcgggttt
    32221 accgaaactg gtcacttctc ttgacgcgat tttgtcgtca gctacgacag cataaaagcg
    32281 acagcatcct cggcatggat cgccgcggta tcaaacacag gcaaaacact gagctcttct
    32341 ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc ctgttctgca
    32401 aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattg ccccagacac
    32461 agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg aataagacaa
    32521 ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgc
    32581 gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc ggtggcatcc
    32641 gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac tttatgcatc
    32701 gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag cgccgcctca
    32761 gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc tatttcatga
    32821 aaatcgacgc tatgtagcag cacttgcgca gagtgaagcc caccaagccg ctgtttaatg
    32881 ccttcattta tcagacgata gtaaggaata gtggattccc agctcattcc tcccagcaaa
    32941 ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac
    33001 gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag ggccggatgt
    33061 acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg cctgccatca
    33121 gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga
    33181 tgcctacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcgccaatgc
    33241 tatcaagtag tgtcaggaag gtcgcgccga taatcatatt cgacacccag tttgtcgtcg
    33301 ttgagcaggt gatgccaaaa tcgcggcatt tcagcggctg aatttcagag cacaggatcc
    33361 acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag
    33421 agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg caatagccca
    33481 gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt ttacgccctg
    33541 ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt cccacgacca
    33601 gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgcggcg
    33661 cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga
    33721 gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta
    33781 acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca
    33841 gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgctagc cagcgcgggc
    33901 tatttggcag gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca
    33961 acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga
    34021 tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg
    34081 ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca
    34141 ccacacgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct atagaaccga
    34201 gtacaaacag gatggccccc gccatcaggc tgtatttacg ccccaggcgg aacgacagcc
    34261 aaccattaaa cagcgcacca attgctgcgc cgagcatcat gctactaacc acccattcct
    34321 gcaaacgact ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc
    34381 cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca
    34441 tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca
    34501 ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ctcacacagg
    34561 tgtcaggtcg gaaacagcat aaatatggat taaattgcta cgacatgtcg ttatgtgatg
    34621 gatattccaa ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta
    34681 cagcagataa aattcataaa gttcattaat tgataattga tatggattat ttcataacca
    34741 tgatatggat tatgatgatc tacaggtata aaaaaccctg ccatgcggca gggtcataaa
    34801 agtaagagaa atgaattaac gcgccagcca accgccatcc acggcaatgg tataaccatt
    34861 cacataatct gaagcgctgg aggcaaggaa cactaccggc cccatcaggt cactcggcag
    34921 tccccaacgg ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg
    34981 tagttgttga gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt
    35041 gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc ataacaccgc tttttgatgc
    35101 ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat
    35161 cttgccgcca ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac
    35221 gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcatc
    35281 ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac
    35341 cgcgcgatcc agcagtgctg gaataccatc aatctttcgc agatcggcgg tcaggcttaa
    35401 aaaacgacgc cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat
    35461 gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagtccagt
    35521 atcacaacca gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac
    35581 aataccttat tcgtttatgc cgacaactag cgcaaatctt taacggccac atggtccata
    35641 tcatcaaaga cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg
    35701 acgccggaat ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg
    35761 tgacgcgttt cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg
    35821 aaatagaaat aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag
    35881 ttacctggag ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg
    35941 acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt tatcccctaa cgtgactgga
    36001 gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tacgcaattg
    36061 taataaaact tcgccggagt ggcggtatca tcactggcaa agacaacttc ttttgcacct
    36121 ttacccacat acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc
    36181 gtaccggcac cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg
    36241 ccgagttgtt taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg
    36301 ccaacaataa tacggtcaat gtggctgtaa accatggtgt actcatcggt gacaaatacc
    36361 ttttcaacca aaaattcatt gcgcagcccc tgggtatcca gtgtttttgc gtgcgcactg
    36421 tggatgctct gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa
    36481 aacgatagtg aaacactgtt tcgttttctc tataagggga tgatatgccc atgggatagg
    36541 gttttcaatt aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgccg
    36601 tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg agcccagaaa atgtatgtcg
    36661 acttgccagc catcattatt caacagatta atgacggcag atatttatcg gtaaggagga
    36721 aatgcagagc tactcgtcac gttcaatggt caatgctacg ccctggcccc cgccgataca
    36781 aagcgttgcc agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag
    36841 gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt
    36901 gacccgacgc tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc
    36961 ttcattagcc tcgataagat cgacatcagc caactgccag cccacacgct ccaggcaacg
    37021 gcgggtcgca tacaccggcg caattcccat caatgctgga tctacaccaa cgctggcaaa
    37081 tgcgcggatg cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat
    37141 cattaccgca gctgcgccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag
    37201 actatcaaat gaaggattta aacgggctaa gccttctgca ctggcgtcag tgcgtggctg
    37261 ttcatcggta tcaataacca acgtctgacc attactttgg gtcattaccg ggacgatctc
    37321 atctttaaat cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta
    37381 agcatcctgc aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc
    37441 aatatgataa tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg
    37501 gctattgcca agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat
    37561 gttttcctgg ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc
    37621 cagatgcagt gccttaagcc cggaaccgca aacgtcatta atcgtgattg cagaaacgct
    37681 attaggaaga ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt
    37741 caacacctga ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat
    37801 taacgctttc acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg
    37861 aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc
    37921 gcaccaatta acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa
    37981 aaaagtgatt tttatcacaa aggaaatatg cctgagcagc agtcagagac ataactggca
    38041 cgtaaggttt gcaaccacta acccaccaat agaggggtag atagggcgtt aatctcccat
    38101 acttaacctg gtttatggta aattgccctc tattttgttt aatttgtaga tgatacgttc
    38161 agataatgtc taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca
    38221 cagccgttgg tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggcagtgg
    38281 tcctgtttat tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat
    38341 gccagttcat cctctcatcg aaaacatcag caggtgaagg gataacgggc gcagtaacac
    38401 actactatgg caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg
    38461 tcgtcgtgtt gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc
    38521 atatggttat tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga
    38581 atctcatttt tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc
    38641 cattgattgc ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc
    38701 tattaacaac ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc
    38761 ccgtgatggt tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac
    38821 gtgaaaaata tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc
    38881 tcatcatctg cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt
    38941 cgtatattga agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc
    39001 cgaacgcccc agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga
    39061 aatcattcct gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa
    39121 cattacagca ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt
    39181 tggtatcgct gatcaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt
    39241 atgcgatcag cggcccactc attgccatga tacttttcat catgcctacg ctgtcaacgt
    39301 atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg
    39361 gtattctgtg cgtatcggta atgttcttta gctaatatcc attgtgaatg gttcaaaatt
    39421 gctacaattt tgaaccattc catttttcat aatgttcatt tgttattatt ccgccctatc
    39481 acttctctca tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat
    39541 cttaaataag attatttaag atgcataact tagattcgca agatatcttc tggtcattca
    39601 aaaacaattt gcgatataga tattctcata ccatgagata gttatctgga ccactctcaa
    39661 ccatgcaaga ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa
    39721 atgtcttaat cagaattata gcaaatacag attaatcgca atttttccca gcaacattac
    39781 aaaatcatgg tgtattgaca tcatcaataa ccaatgaaat tatgcaatta tatacggata
    39841 gggaggttct taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct
    39901 caatgcattg ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt
    39961 gtttttgtat gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct
    40021 taacaaaaaa ccacaatgcg agattaagcg gatatatttt tgtcgatttt tcagtaagtt
    40081 ttttacgtct attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga
    40141 ttgttttagt cagcgaccgc aatatgcaat cgttagctaa ctactggcga aagcacaact
    40201 ctgcaatatc tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca
    40261 gacaactatt tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg
    40321 aattcaccat aatggggtat atggtttctg gttataaccc atatcagatt gctgaagttc
    40381 tggatatgga tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg
    40441 gtaaaataaa cgagttattt attcgttcac attcggtcca acattaatac tcaacgatct
    40501 gccagaatca tactctgctt atacgggcat ctattggaat gcccgtattt tataaataag
    40561 cagtaacccc aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc
    40621 attacgaaat atgaatttat caacaatagt ggcataaatg ttaaccatgt taatttatat
    40681 aaagttttac gttgcgccat taaagcctca tttcaatcat catgataaat ataaaattaa
    40741 tatatattta tgccgtaaat accaatatac ttagcaaact atgtgatttc catttcgatt
    40801 gatttagtgt ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg
    40861 agcaccgaaa attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag
    40921 ccagtcgttg tttatccctt ctacttgaag cttttcctga tgtggttgca caacaagatt
    40981 ttttcaccag agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga
    41041 acatatccat tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgcttattg
    41101 caaccgtgcc gagaagagga ttcaagatcc ataatgacat taacatacaa aatcatgtaa
    41161 taaactcgtc aacagacgca catacacaca atgccccacc ggccataaaa gttaatgcgg
    41221 gatacaaaga gagcattggt ggcgcaaaga atttcaataa caaaatcctc aaacatataa
    41281 aaagttatct aattatgttg agcgcatttg tcataggcgc atattctgca tattggctat
    41341 ggaataataa tcaaccgaag ccatttttca aggattacaa gactgttgcg gaaattaatg
    41401 gctgccattt taatgtaaca gaagatacaa ttgatgggtt gaaagagttc gataaatata
    41461 aaacacggat actggattcg gggattaact gtaaaaaata tccgtggtta tacttccctc
    41521 ttgcaaaatc ctcaccgggg atgattgtta tggcatgcaa taaaaactat aaccaacatg
    41581 aagtggcagg ctgtttaacc ctgtcttacc gtgaggttaa tcgtgattga ttataaaaaa
    41641 aacttattat ttattttagt ttttatcagt gggtttattc tcttcactgt ctattcttac
    41701 actgcagaaa aaatgatata taacgaaaca tgcacttcaa attgggtgat ttttaatgac
    41761 caggggcgag caaatcttac tatcgacttt atgtataacc aaaaaaataa aacaggaact
    41821 gttgcgttga gtgggacctg gcaacaaggt aacagagaaa gtaaatcaat acggcgaaat
    41881 attgaataca catgggttga aaactatgac acagcccatt taacttcaaa aaaagttaat
    41941 aagtttgata ttatggatca agtcgatgat gatagacttg ctgaacttat tcctgatttt
    42001 tatgtctttc cagaaaaaag tgtaagctat aatatactaa agcaaggtaa acatgctttt
    42061 attttgagta ttggtaacag agcaataatg cattgtgcaa ggtaaactaa aatagataac
    42121 aaaaagatgg aacctcgtta atcataaaat aataaaatta aatatgattg cagtatatgc
    42181 cagtataaag catcaaagtt ttgatcaaca tctttcattt tagacatcac cttgcaatgt
    42241 aaaatatcat gtatcaacaa catctggtgc atatccatga gtcgaactca acattcatct
    42301 tagctcgatt attattcctt cgtacagtcg atgtaaacaa tacagaaaga ggattattaa
    42361 gaaccagttt actcagtgaa tcaatagagg aaaggattaa cgtttcttta aaagaattaa
    42421 tttcatcatc tgttaaacta aactcatcat tgacagatcg tgagatataa ctgtttttaa
    42481 ctttactctt tacgttgact ttactgacag aattaacatt cacatatctt gaatttaatg
    42541 cccatgatgt tgtttgcgtg agaaccttct cagcattaag gaattttttt aacggaatcc
    42601 ttggtttctt tttcgagtcc gaattaacta tatcatgcat atagaccatc tcatgaatct
    42661 gcgaaaactc aatgtccatt atatacctca catttttacc gtgactcgat gttactgttc
    42721 aataatcacc ttccatcaat actaaaatta ataccccaaa tgtgccgata acaaatatag
    42781 tcattctacg taacgtctcc ataaggtgat aaccttgatc aaatgaattt tgaatataat
    42841 aaaggaaatc ttttgagatg tttatcacga ataacgctat ttatgttacc aatatcattt
    42901 cgagtgcgca cactatccat ctgtttttta aggatataaa aaacaacatg taagttatgt
    42961 aaattgccga tgctactaag taacattatg gtaaaaaaag taaaaatgag cgatttttga
    43021 tagaataaag caagagaaaa ggtgatattt gacattatca gaagctgcga attcggattt
    43081 tgctctaatc aaatgagaga aatgtaaaat gaagccacga aatattaata atagcctacc
    43141 actgcaacca ttagttcctg atcaggagaa caaaaataag aaaaatgaag agaaattcgt
    43201 taattcagtt aaaattacca tggggtctgg tttaaattat attgaacaag aatctcttgg
    43261 aggaaaatac ctaacacatg atttgtcaat aaagatagcg gatatttctg aagagataat
    43321 tcagcaagca atattatctg ctatgtgcat atataaatta ccgattacag atgatttaat
    43381 gagtatggct acaaatgaac tcataaaact gaccaaaata gagaataatt tagacctgaa
    43441 taaattcgct actatatgca cagacgttct atcccccctc gtcaccagac ataataaaca
    43501 aaaaaaccaa aacgacattc tacccttcgc aaaaatcccc tttttaattt tcattgaaaa
    43561 aaaggaaatt gataatatga atccaggtca tgcacattaa ggcattttga aatgtcgtaa
    43621 ttaagataac taataaggtg aatattagta aaacaactaa tgatgttatt attttgtagc
    43681 ggtaagtatt tttactaata acgaacaaat atactcataa gcaatgcaag cttaaaacta
    43741 ataatttaaa aaag
//
LOCUS       DAZGCQ010000033        43391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000033 DAZGCQ010000000
VERSION     DAZGCQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.033"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..120
                     /locus_tag="ACHK07_001932"
     tRNA            44..120
                     /locus_tag="ACHK07_001932"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Met,seq:cat)
     gene            169..243
                     /locus_tag="ACHK07_001933"
     tRNA            169..243
                     /locus_tag="ACHK07_001933"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:201..203,aa:Gln,seq:ctg)
     gene            281..355
                     /locus_tag="ACHK07_001934"
     tRNA            281..355
                     /locus_tag="ACHK07_001934"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:Gln,seq:ctg)
     gene            complement(509..1684)
                     /gene="ubiF"
                     /locus_tag="ACHK07_001935"
     CDS             complement(509..1684)
                     /gene="ubiF"
                     /locus_tag="ACHK07_001935"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO8102268.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1830..3254
                     /gene="miaB"
                     /locus_tag="ACHK07_001936"
     CDS             1830..3254
                     /gene="miaB"
                     /locus_tag="ACHK07_001936"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO8102269.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3407..4447
                     /gene="ybeZ"
                     /locus_tag="ACHK07_001937"
     CDS             3407..4447
                     /gene="ybeZ"
                     /locus_tag="ACHK07_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO8102270.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4444..4911
                     /gene="ybeY"
                     /locus_tag="ACHK07_001938"
     CDS             4444..4911
                     /gene="ybeY"
                     /locus_tag="ACHK07_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO8102271.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            5001..5879
                     /gene="corC"
                     /locus_tag="ACHK07_001939"
     CDS             5001..5879
                     /gene="corC"
                     /locus_tag="ACHK07_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO8102272.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5904..7442
                     /gene="lnt"
                     /locus_tag="ACHK07_001940"
     CDS             5904..7442
                     /gene="lnt"
                     /locus_tag="ACHK07_001940"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO8102273.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWSKTTWLRVAIVAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7839..8747
                     /gene="gltI"
                     /locus_tag="ACHK07_001941"
     CDS             7839..8747
                     /gene="gltI"
                     /locus_tag="ACHK07_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO8102274.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8917..9657
                     /gene="gltJ"
                     /locus_tag="ACHK07_001942"
     CDS             8917..9657
                     /gene="gltJ"
                     /locus_tag="ACHK07_001942"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO8102275.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            9657..10331
                     /gene="gltK"
                     /locus_tag="ACHK07_001943"
     CDS             9657..10331
                     /gene="gltK"
                     /locus_tag="ACHK07_001943"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO8102276.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10331..11056
                     /gene="gltL"
                     /locus_tag="ACHK07_001944"
     CDS             10331..11056
                     /gene="gltL"
                     /locus_tag="ACHK07_001944"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO8102277.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11174..12109
                     /gene="rihA"
                     /locus_tag="ACHK07_001945"
     CDS             11174..12109
                     /gene="rihA"
                     /locus_tag="ACHK07_001945"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO8102278.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHGEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            12193..13863
                     /gene="hscC"
                     /locus_tag="ACHK07_001946"
     CDS             12193..13863
                     /gene="hscC"
                     /locus_tag="ACHK07_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO8102279.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHTSAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(13923..15374)
                     /gene="djlC"
                     /locus_tag="ACHK07_001947"
     CDS             complement(13923..15374)
                     /gene="djlC"
                     /locus_tag="ACHK07_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO8102280.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDDATEHEILRVFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSVGMDEEEREAFLEAI
                     QAGDCFDFLGLLEYPVALQNQTVEYYFALERCCHYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15371..16078)
                     /gene="ybeU"
                     /locus_tag="ACHK07_001948"
     CDS             complement(15371..16078)
                     /gene="ybeU"
                     /locus_tag="ACHK07_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8102281.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            16180..16734
                     /gene="ybeT"
                     /locus_tag="ACHK07_001949"
     CDS             16180..16734
                     /gene="ybeT"
                     /locus_tag="ACHK07_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8102282.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16744..18171)
                     /gene="djlB"
                     /locus_tag="ACHK07_001950"
     CDS             complement(16744..18171)
                     /gene="djlB"
                     /locus_tag="ACHK07_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO8102283.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEECEVAEHEILLAFSALLASDSERFLPSAWQRFIQQLNY
                     CSMDEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGKSLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18168..18875)
                     /gene="ybeR"
                     /locus_tag="ACHK07_001951"
     CDS             complement(18168..18875)
                     /gene="ybeR"
                     /locus_tag="ACHK07_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8102284.1"
                     /translation="MDMKSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            19039..20016
                     /gene="ybeQ"
                     /locus_tag="ACHK07_001952"
     CDS             19039..20016
                     /gene="ybeQ"
                     /locus_tag="ACHK07_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO8102285.1"
                     /translation="MIFTSSCCDNLSIDKIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(20086..20568)
                     /gene="ybeL"
                     /locus_tag="ACHK07_001953"
     CDS             complement(20086..20568)
                     /gene="ybeL"
                     /locus_tag="ACHK07_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO8102286.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            20804..23386
                     /gene="leuS"
                     /locus_tag="ACHK07_001954"
     CDS             20804..23386
                     /gene="leuS"
                     /locus_tag="ACHK07_001954"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO8102287.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23401..23982
                     /gene="lptE"
                     /locus_tag="ACHK07_001955"
     CDS             23401..23982
                     /gene="lptE"
                     /locus_tag="ACHK07_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO8102288.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23982..25013
                     /gene="holA"
                     /locus_tag="ACHK07_001956"
     CDS             23982..25013
                     /gene="holA"
                     /locus_tag="ACHK07_001956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO8102289.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            25015..25656
                     /gene="nadD"
                     /locus_tag="ACHK07_001957"
     CDS             25015..25656
                     /gene="nadD"
                     /locus_tag="ACHK07_001957"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO8102290.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            25680..26291
                     /gene="cobC"
                     /locus_tag="ACHK07_001958"
     CDS             25680..26291
                     /gene="cobC"
                     /locus_tag="ACHK07_001958"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO8102291.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26551..26868
                     /gene="rsfS"
                     /locus_tag="ACHK07_001959"
     CDS             26551..26868
                     /gene="rsfS"
                     /locus_tag="ACHK07_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO8102292.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26872..27339
                     /gene="rlmH"
                     /locus_tag="ACHK07_001960"
     CDS             26872..27339
                     /gene="rlmH"
                     /locus_tag="ACHK07_001960"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO8102293.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27370..29271
                     /gene="mrdA"
                     /locus_tag="ACHK07_001961"
     CDS             27370..29271
                     /gene="mrdA"
                     /locus_tag="ACHK07_001961"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO8102294.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29274..30386
                     /gene="mrdB"
                     /locus_tag="ACHK07_001962"
                     /gene_synonym="rodA"
     CDS             29274..30386
                     /gene="mrdB"
                     /locus_tag="ACHK07_001962"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO8102295.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30397..31485
                     /gene="rlpA"
                     /locus_tag="ACHK07_001963"
     CDS             30397..31485
                     /gene="rlpA"
                     /locus_tag="ACHK07_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO8102296.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLSATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLRQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31625..32836
                     /gene="dacA"
                     /locus_tag="ACHK07_001964"
     CDS             31625..32836
                     /gene="dacA"
                     /locus_tag="ACHK07_001964"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO8102297.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32947..33210
                     /gene="ybeD"
                     /locus_tag="ACHK07_001965"
     CDS             32947..33210
                     /gene="ybeD"
                     /locus_tag="ACHK07_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO8102298.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33311..33952
                     /gene="lipB"
                     /locus_tag="ACHK07_001966"
     CDS             33311..33952
                     /gene="lipB"
                     /locus_tag="ACHK07_001966"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO8102299.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34212..35165
                     /gene="ybeF"
                     /locus_tag="ACHK07_001967"
     CDS             34212..35165
                     /gene="ybeF"
                     /locus_tag="ACHK07_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO8102300.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35374..36339
                     /gene="lipA"
                     /locus_tag="ACHK07_001968"
     CDS             35374..36339
                     /gene="lipA"
                     /locus_tag="ACHK07_001968"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO8102301.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAAALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36440..36643)
                     /gene="tatE"
                     /locus_tag="ACHK07_001969"
     CDS             complement(36440..36643)
                     /gene="tatE"
                     /locus_tag="ACHK07_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO8102302.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36772..37560)
                     /locus_tag="ACHK07_001970"
     CDS             complement(36772..37560)
                     /locus_tag="ACHK07_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO8102303.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILALPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            37653..38036
                     /gene="crcB"
                     /locus_tag="ACHK07_001971"
     CDS             37653..38036
                     /gene="crcB"
                     /locus_tag="ACHK07_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO8102304.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(38090..38299)
                     /gene="cspE"
                     /locus_tag="ACHK07_001972"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38090..38299)
                     /gene="cspE"
                     /locus_tag="ACHK07_001972"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8102305.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38474..39034)
                     /gene="pagP"
                     /locus_tag="ACHK07_001973"
     CDS             complement(38474..39034)
                     /gene="pagP"
                     /locus_tag="ACHK07_001973"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO8102306.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLANENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39623..41008
                     /gene="dcuC"
                     /locus_tag="ACHK07_001974"
     CDS             39623..41008
                     /gene="dcuC"
                     /locus_tag="ACHK07_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8102307.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(41048..41728)
                     /gene="dpiA"
                     /locus_tag="ACHK07_001975"
                     /gene_synonym="citB"
     CDS             complement(41048..41728)
                     /gene="dpiA"
                     /locus_tag="ACHK07_001975"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO8102308.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(41697..43355)
                     /gene="dpiB"
                     /locus_tag="ACHK07_001976"
     CDS             complement(41697..43355)
                     /gene="dpiB"
                     /locus_tag="ACHK07_001976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO8102309.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGHPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT        10876 a        11034 c        10769 g        10712 t
ORIGIN      
        1 cctggttcga atccaggtac cccagccatc gaagaaacaa tctggctacg tagctcagtt
       61 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
      121 aattctgaat gtgtcgaata tgttcgacaa attcaaaaac caatttgttg gggtatcgcc
      181 aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag
      241 ccaatttatt caagacgctt accttgtaag tgcacccagt tggggtatcg ccaagcggta
      301 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta
      361 aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc actcaatgtc gaatgcgatg
      421 ttgacacgtc ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg
      481 ttggcacgca tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac
      541 gtttcaacac gccagcacgc tccgccgcca tcaaaccaag attacgcaca aaacgcagcg
      601 gcggcagatt attactgaat ccggcataaa acagatccat accgctctgc ataatgaagt
      661 tatccgccat gcgccgcatc tggtaacgct tgaggacagg ataactggcc cacgcttcgc
      721 cgtagctgcg ggcattcacc agaacatcaa tcagggcatc gacatcacga taaccaagat
      781 tcaccccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc
      841 ctggctgcac atactgcaac gcatggcgac gcgtcagcgg aaacgcacca gcggcaagcg
      901 gtgtaacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgg agctgtgcca
      961 tattcatatt ctgcaactgg cgaatacgcg ccggagagtc ataccacacc agcgatgccc
     1021 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg agtaaattgc tgccaggtgc
     1081 tatcgccggg atcgttctcg cactggacgc taatcaacat gcacgactgc gcatactgcc
     1141 atgcatgaac gccaattccc gccatctgcc gcacctgcga atttgcgccg tcggcaccaa
     1201 tcaccagctt cgagcgaatc acttcaccgc ctttcagctc cagctcctga agatcattat
     1261 ctcgatgtaa tgcaatcagc gagcctggca cacgtaacgt tactttcgga tgcgcttcca
     1321 gcgcctgcca caacgcctgt tgcaggacag tgttttccac catatagcca agcagcggta
     1381 gcttaagttc agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc
     1441 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
     1501 atgataccga agccgcgctg atcgccgaga tccgcacgtc cggttggcta tcagcgacaa
     1561 acggtgcagg ttctgcgtgc tcgatcaccg ttaccgtaaa tccgtgctgt gccagcccca
     1621 gcgccagtgc gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg
     1681 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
     1741 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt
     1801 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
     1861 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
     1921 gctatcaact gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa
     1981 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
     2041 agaagaatcc agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc
     2101 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc
     2161 tgccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc
     2221 cggaaatcga gaagtttgac cgtctaccgg aaccgcgcgc cgaagggccg accgcgtttg
     2281 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg
     2341 gtgaagaggt aagccgtccg tccgacgata ttctgtttga gattgcccag cttgcggctc
     2401 agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact
     2461 acgacggcac caccggatcg tttgccgatc tgttgcgtct ggttgctgcg atcgacggga
     2521 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
     2581 tgtatcgcga cacgccggag ctggtgagct tcctgcatct gccggtacag agcggttccg
     2641 atcgcattct gaacctgatg ggacgtaccc atacggcgct ggagtacaaa gcgatcatcc
     2701 gtaaactgcg tgcggcgcgt ccagatattc agatcagttc tgatttcatc gttggcttcc
     2761 ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
     2821 acatgagcta cagctttatc ttctctgcac gtccgggtac accagccgcc gatatggttg
     2881 atgatgtccc ggaagaagag aagaagcagc gtctgtatat tctgcaagag cgcattaatc
     2941 agcaagcgat ggcgtggagc cgccggatgc tcggcaccac ccagcgtatt ctggtagaag
     3001 gtacatcgcg taagagcatc atggagcttt ccgggcgtac ggaaaataac cgcgtggtca
     3061 acttcgaagg cacgccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
     3121 acccgaactc tctgcgcggt aaagtggtgc gaactgaaga tgaaatgggt ctgcgcgtgg
     3181 cagaaacacc ggaatcggtg atagcccgta ctcgcaaaga aaacgacctt ggcgtgggtt
     3241 attatcagcc gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat
     3301 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
     3361 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
     3421 tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg ttgagcctgt gcggcccgtt
     3481 tgatgacaac atcaagcagc tcgaacgccg tctcggcatc gagatcaatc gccgcgataa
     3541 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
     3601 cctgtatgtc gatactgccc cgatgcgcgg tcagattcag gatattgaac cggaacagat
     3661 ccaccttgcg attaaagaag cgcgggtatt ggagcaaagt gcggagagcg tgccggagta
     3721 cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca
     3781 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
     3841 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggaaat
     3901 tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc
     3961 tggcgattta agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga
     4021 aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcgcc
     4081 gctggcctat atgcgtggtc gtacgctgaa tgacgccttt atcattctcg atgagagcca
     4141 gaacaccacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
     4201 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggcttacg
     4261 tcatgccatc gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga
     4321 agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcctatgaag cctgggaaga
     4381 agccgaacaa aaacgtaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca
     4441 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataactc cggattgccg
     4501 gaagagagcc agtttcaaac atggctgaat gcggtgatcc cgcagtttca ggaagagtcg
     4561 gaagtgacga ttcgcgtggt cgataccgcc gaaagccaca gtctcaatct gacctatcgc
     4621 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggaa
     4681 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
     4741 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
     4801 ttaggttacg atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag
     4861 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagc
     4921 catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac
     4981 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
     5041 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
     5101 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
     5161 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgtccgc gacatcatga
     5221 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
     5281 tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
     5341 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
     5401 gcatggacaa agtgttacgt caggcagttg tcgttcctga aagtaagcgc gtagaccgga
     5461 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgttattgac gaattcggtg
     5521 gggtttccgg tctggtaacc attgaagaca tcctggaact gattgttggt gagattgaag
     5581 acgaatatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
     5641 tgcgcgcact ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
     5701 aagaagtcga cactatcggt ggtctggtga tgcaggcatt tgggcatctt ccggcacgtg
     5761 gcgaaaccat cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcggcgta
     5821 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccgaagctg gatgaataaa
     5881 accgaaactg gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc
     5941 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
     6001 tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc gctgaccttt aaccgccgtc
     6061 cactccagtc tgccgctatt ggcttttgct ggggatttgg cctgtttggc agcggtatta
     6121 actgggtcta tgtcagtatc gcgacctttg gcggaatgcc tggcccggtt aacatcttcc
     6181 tggtggtgct gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgttgt
     6241 cgcgtctgtg gtcgaaaacc acctggctgc gcgtggcgat tgtcgctcct gctctctggc
     6301 aagtgaccga gtttctgcgc ggttgggtgc tgactggctt cccgtggtta cagttcggct
     6361 atagccagat tgatggcccg ttaaaagggc tggcaccgct aatgggcgtg gaagccatta
     6421 acttcctgct tatgatggtt agcggcctgc tggcactggc gttagtcaaa cgcaactggc
     6481 gtccgctggt ggtggccgtc gtgctgtttg cccttccctt cccgctgcgt tacatccagt
     6541 ggtttacccc acaaccggag aaaaccattc aggtttcgat ggttcagggc gatattccgc
     6601 aatcgctgaa atgggacgaa ggccagcttc ttaatacgct gaagatttac tacaacgcaa
     6661 cggcaccgct gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc
     6721 tggaaattaa tcagcaaccg ttcctcaaag cactggacgg tgagttgcgt gataaaggga
     6781 gtacgctggt gaccgggatt gttgacgcgc gtcttaataa gcagaaccgt tacgatacct
     6841 acaacaccat catcacactg ggtaaaggtg caccgtacag ctacgaatca gccgatcgct
     6901 ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc
     6961 cgttagcacc gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc
     7021 cgccgctgtc ggcaaatggt attgagctta ctgcggctat ttgctacgag atcattctcg
     7081 gcgagcaagt gcgcgataac ttccgcccgg ataccgacta tctgctgact atctccaacg
     7141 atgcgtggtt tggtaagtct attggtccat ggcagcactt ccagatggcg cgaatgcgtg
     7201 cgctggagct ggcgcgccca ttgctgcgta gtaccaacaa cggcattacg gcggtgattg
     7261 gtccgcaggg tgagattcag gcgatgatcc ctcagttcac ccgcgaagtg ttgaccacta
     7321 acgtgacgcc gaccaccgga ctcacaccat acgcacgtac cggcaactgg ccgctgtggg
     7381 tgctgacggc actgtttggt tttgccgctg tgctgatgag tctgcgtcaa cgacgtaaat
     7441 aatcccttct tatcgtgccg gaatacatcc cgtatttcgg cacgctcacc ggattcggca
     7501 tcagtgcgtt atccggataa caaaattccc ctatccaact ttggcacatc tattgctttg
     7561 ttatacaagg caaagcttga accagcatca gtacaacccg gttgcaccac agcaatccac
     7621 cggtagcacc aaaagagtgc attttaagaa ttttgcttcg gaatggtgcg gcgcacctcc
     7681 caaatataaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
     7741 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
     7801 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
     7861 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
     7921 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
     7981 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
     8041 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
     8101 tgaaactgat tccgatcacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
     8161 attttgaatg tggttccacc accaataacg tcgaacgcca aaaacaggcg gctttctctg
     8221 acactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
     8281 ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
     8341 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg
     8401 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
     8461 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc
     8521 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
     8581 tgatggatga cactatcgct caggtgcaga cctccggtga agcggaaaaa tggtttgata
     8641 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
     8701 aaatgaaagc actgttcaaa gaaccgaatg acaaagcact gaactaatta caagaaccag
     8761 gggcggaaat tctagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
     8821 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
     8881 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
     8941 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
     9001 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
     9061 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
     9121 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
     9181 ccggagttgc tgccagagaa aatcggcatg tggtttaagg ccgagctgga cccaaatatt
     9241 cagtttttcc tctcctccat gctctgtctg gggctgttta ccgccgcccg tgtttgcgaa
     9301 caggtacgcg ccgccattca gtcgctgccg cgcgggcaaa agaatgccgc gctggcgatg
     9361 gggctgacgc tgccgcaggc ttaccgctat gtactgttgc ctaatgctta tcgcgttatc
     9421 gtcccgccga tgacctccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
     9481 atcggtctgg tagatatggc agcgcaggcg ggtaaattgc tcgattactc ggcccacgcc
     9541 tgggagtcgt ttaccgccat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
     9601 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
     9661 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
     9721 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
     9781 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
     9841 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccgggtt
     9901 ttctgcaaaa cgtgctggga ttatcgccaa aaaatgatat ccgtttaatt tcggcgatgg
     9961 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    10021 gtatttctcg tggtcagtct agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    10081 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgctgctg ctcactcagg
    10141 gcatcgtact gttccaggat acctcgctgg tgtatgtgtt atctctggcc gatttcttcc
    10201 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    10261 gatttgttta tttcgttatt agccttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    10321 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    10381 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    10441 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccggtgc agcaaggtga
    10501 aatcaccgtc gatggtatcg tggttaacga taagaaaacc gatctggcaa agctgcgttc
    10561 tcgcgtcggg atggtattcc aacatttcga gctgttccct catctgtcga ttatcgaaaa
    10621 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcaccggctc gtgaaaaagc
    10681 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    10741 ttccggcggc cagcagcagc gtgtggcaat tgctcgcgca ttgtgtatgg atcctattgc
    10801 gatgctgttc gacgaaccca catcggcact agacccggag atgatcaacg aagtactgga
    10861 cgtgatggtg gaactggcga acgaggggat gaccatgatg gtggtgaccc acgaaatggg
    10921 ctttgcccgt aaagtggcga atcgggtgat ctttatggat gagggtaaaa ttgttgaaga
    10981 ctcgccgaaa gatgcgttct tcgatgatcc gaaatcggac cgcgcaaaag acttcctcgc
    11041 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    11101 tcgtctcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    11161 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgatgacgc
    11221 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    11281 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    11341 taatcgcacc gatattccgg tggcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    11401 tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc ccggcattac cggaaccgac
    11461 gtttgcaccg caaaagtgta cggcggtgga gctgatggcg aaaacgctac gtgaaagtgc
    11521 ggaacctgtc accattgttt ctaccggacc gcaaactaac gttgctttgc tgctcaatag
    11581 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    11641 gggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccagaag cggcagaaat
    11701 tgtattccag tcagggatcc cggtggtgat ggccggtctg gatgttactc acaaagcaca
    11761 aattcacggt gaggacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    11821 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    11881 cgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    11941 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat acccagggga tgacggttgt
    12001 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    12061 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    12121 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctctt tttctttcaa
    12181 gatataaatg caatggataa tgcagaactc gccattggta tcgatctcgg tactaccaat
    12241 agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa
    12301 tatttaacac catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg
    12361 gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgtgcaatg
    12421 ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct
    12481 ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat
    12541 gtggtgatct ctgttccggc ttatttcagc gatgaacaac gcaagcatac ccgtttagca
    12601 gcggagttag ccggtttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg
    12661 gcgtatggcc tgcataccca acaaaatacc cgttcgctgg tgtttgatct cggtggcggc
    12721 acgtttgacg ttacggtgct tgagtacgcc acgccggtga ttgaagttca cacctccgct
    12781 ggcgacaact tcctcggtgg cgaagatttt acccatatgc tggtcgatga ggttttaaaa
    12841 cgcgcggatg tcgccaggac cacgcttaac gagagtgaac tggcagcctt gtatgcctgt
    12901 gtggaagcgg caaaatgtag caatcaatcg ccaatgcaca ttcgctggca gtaccaggat
    12961 gaaacgcggg aatgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc
    13021 aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cacgtctgaa gccgagtcaa
    13081 atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc
    13141 gtgcgtctgt ttggcaaatt accgtatcaa agttacgatc cgagcaccat tgtcgcgctg
    13201 ggcgcagcaa ttcaggctgc ctgccgctta cgcagtgaag atattgaaga agtgatcctc
    13261 actgatattt gcccttactc gttgggcgtt gaggttaacc gtcagggcgt ttccggcatt
    13321 ttctcgccga ttattgaacg aaacaccact gtgcccgtgt cgcgtgtaga aacttattca
    13381 accatgcacc cggaacagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa
    13441 gttaaaaaca acattctggt ggaatccttc gatgtgccgt tgaagaaaac cggggcttat
    13501 cagtcgattg atattcgctt tagttatgat atcaacggtt tgcttgaagt tgacgtgctt
    13561 ctggaagacg gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg
    13621 cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg
    13681 ctcatcaatc gtacctttaa agccaaactg gaagagttgt gggcgcgggc gctgggtgac
    13741 gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgctgca gtcaaacgat
    13801 atggcccgcg tcgatgaggt caggcggcgg gcgagcgttt atttagccat tgagatccca
    13861 taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat
    13921 cgtcaactaa acaacatcgc gattttgccg agagcataac ctataaccag caccgttaaa
    13981 ctgattttga taatgctggt aatgctctgt ggtttttcac cctcatcatt tttttcttca
    14041 tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt tactttcgtc
    14101 aacagcatcc gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta
    14161 ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa gtactacctg ttccgctacc
    14221 cgatacaaat ccagtaacgg tgaatcttgc tctgtctcgc taccgactct cgatttccac
    14281 cacgcccatg ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat
    14341 agttttccgc tatgtaaaat ttccgcccat aacagacgcg tccgggcgct ctcaccagga
    14401 acatatttca acggtttacc ttgcgcatct accatgctcc gcgcttcgat aaccatcact
    14461 aataacgcga aataatccgg gcaatgttgt gcgctccact gcaacaacaa attatcggct
    14521 tgtgtagatg ggtaacttcc ccagtacgcg cagagatcgg caagcaggct ttccccctcg
    14581 ccgcagtaca aacgttgtgc acactggtaa taccgggcat cttcgctttc tggttcttcc
    14641 atttgccact gtttggctac aggaatgagt tccgccatac ctgtttgcac cgagctgtac
    14701 cagcgcaaca gtttgcgatg taactttgca tcatcaggaa tgaaccacgg accttccatc
    14761 gccaaaaaag cagtgacata gtcaggatgg taatggcagc aacgttccag cgcgaagtaa
    14821 tattcaacag tctggttctg caacgcaacg ggatattcca gaaggccaag gaaatcgaaa
    14881 caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattcctacg
    14941 ctgtcattaa acgaatgcca gttcaaacgc tctgccagca aagaagcaca atttaatgaa
    15001 agatatcgcg cttctatagc gatggcacac agcggccagc gtaattgatc gacatcctcc
    15061 atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg aaaacgatca
    15121 ctttctgaat ccagtaatgc cctgaataca cgtagtattt catgctcagt ggcatcatct
    15181 ttttcttcat catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag
    15241 gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacataa cggtaagcga
    15301 gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc
    15361 caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag
    15421 ttaatggctg ccagggaagc gtggcataga aacgatcgct tcccgctgcc agttcttcga
    15481 acatacgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagtgt
    15541 tgtcttcgca ctgagaagat tgccagtaga gacgccctag tgaataggca gaaaaatagt
    15601 gcatccagct gtgatagtaa tggtgagctc gtacatagac gcgcgattgc agccataaac
    15661 tctcgtcttc cgtcagccac ttgttgagca caccgacccg tgacagaaac cccattcgaa
    15721 cataatccca agctttgatt cccccttctc cacagcacat agcggtttgg gcaacgaatt
    15781 gggtataaat caaccctctc tccgagccac cagcaattaa tgctttccac tcttcagggg
    15841 aagagcgatg ccatgtcaga tataagccag ctaaatcatt agcatgacca tcatcagtca
    15901 tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat gatgagagat
    15961 cgaattcttt tatcccggta tacatttttg gcgagtcatg cgcgggtttg caaccctgat
    16021 taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc ttattcattt
    16081 gttaataatc ccttaagtgc attaactgac tactttttta ttattgatga gtataaactt
    16141 gacgctttca aaataaaaag aaaatcgaag cattcacaca tgaataaaaa attaatgtat
    16201 atattcgcaa tttttatagt tgcagcaatt acctgtatta gccaacccaa gaaaacgacg
    16261 ttgcgtgata aagccatggt gaattatgcc tttgattatt taagctcacc gggcagtctt
    16321 ccattcacca cggcagccac ggagctttcc gcgattcatg gtcactcaac gtcgcaatat
    16381 cgccttggag aattttatct tcatggtagc gacggtaaac cactggatta tacacaggcg
    16441 agatactggt atgagcaatc agcggaacag gaaaatccac gcgcgcaaag taaactgggg
    16501 tggatctacc tcaaaggtct gggggtcaaa cccgacaccc gtaaagcaat tctctggtat
    16561 aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctttaggttt gatctacaga
    16621 aatggctcag gtattaatgt taaccattat gaatctcaaa aatggttaaa actggccgcc
    16681 aaacaacatt acaaaaatgc ggaaagatta cttgccgggc ttcccgcaca ttaacgtcga
    16741 ctgtcacccg aacagatgga gtattttccc tatcaaacca gcaaagataa aaatatagaa
    16801 aataatttta atgatgccca gacagcctgg cccaccatcg tcaacgggca ttggctgcgg
    16861 ttgcggctcc ggttcaacag tttgcttctc ggtaaaattc gccgcctgaa ttaataatcg
    16921 acgaataatt aatgcttcca gtggacaacg attttctgca tcaagacggc ttacgatcgg
    16981 cattaatgat tcttctgttg gttggctggc ctgcactaat tgtttggcaa ctaaatataa
    17041 tggccataag ggatgttctg tatcacgcgt gagtgagatc cgcggcttac gtcctttgtg
    17101 caaaaccatc tcgaccagag aacgccccag cgcagagagt ttttcatcac tgagtatttc
    17161 gtataaatga aaacgcgtac gagcgctgtc accagggaca taaagtagcg gttttccctg
    17221 atcgttaacc agatcacgcg cttcaatcat catcaccact aatgggtaat aatcgacccg
    17281 atgctgacgg caccattgca acattaaagc atccgcctgg gtggagggat attcgcgcca
    17341 gtagtcacaa agttctgcca gcaggctttt gccttctcca caatagacgc gttgcgcgta
    17401 ttcataataa cgcgcatctt cattatcagg ttcagcttcc cgccacgacc gggcgtaatc
    17461 caggagttcg ggaattccca ggcgacctgc gctataccag cggagtaaat ttctgtgcag
    17521 ttttgcatca tcggggataa tcactgcgcg atgttgcgct aaatacaatg tcagccattc
    17581 tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac
    17641 ggcaacgggc agatgcagaa tggtctggaa gttaaaaaca ttccccttcg caatggcata
    17701 taaaaaggat tccagttctt cttcgtctat ttccccggta ttttcctcct gtaaccaccg
    17761 caatctttct gctaataaca ccacgcactc gaaggataaa tgggcagtgt tcatggctat
    17821 tgtgcacagc gaccagcgta attcatcaat ctcatccatc gagcaataat ttaattgctg
    17881 aatgaatcgc tgccaggcgg agggcagaaa acgttcacta tcagaggcaa gtaacgcact
    17941 aaaggcgagc agaatttcat gttctgctac ctcacattct tctggttgcc aaacagattt
    18001 agcaggcgac tgcgcaatcc ggagcgcttc ctcatacgcc tgacgaagtt gtttaaaacc
    18061 ctgcggatcg gtttctggat gaaaggacgg taacagcgcc agataagcgc ggcggataat
    18121 atcgacgtca gtcgtttcct ctatgttgag gatcttccag caatttttca taggtcgctc
    18181 atatccttaa gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca gggtaaggtc
    18241 gcgtaatatg catcgtcact ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg
    18301 tattctttac gcgctaagaa ttcacgtaat aaaggcagat tatcatcttc gggagactgc
    18361 caatattgcc gaccaaaggt ataaccggca aagtattgcc gccagttact ataataacgt
    18421 agagcccgta gatgaatacg cgactggatc catagacttt cttcttcact caaccagtta
    18481 ttaagtacgc ccattcgact taaaaaaccc atccggacat aatcccatgc tttaattcct
    18541 ccctcaccac agcattcggc tgtgctggcg acaaattgcg cataagcctg accttgctca
    18601 tttaattcag caagatagtc acgccattca catggagaat aacgaaacca gcggtgataa
    18661 aaccctgcca accgagcggc atgaccgtta tcgatcattc ggtgaattgt gcgcagcagg
    18721 tcatcacggt catcaattcc ccaggaggaa gaaaggtcaa aaaagcatgt acctaaatac
    18781 attttggggg atgaatgcga aggtaaacag cattcattgc gtatttccat cggagtggaa
    18841 agtgcaaaca atattttttg cgacttcatg tccatataat ttatccttaa acattcattt
    18901 agtgaatgta aactacatta cgaggctcat ttatttatac ttttctgaca caacataata
    18961 cgcataaaat aagacttacg caaaaacgga acacataaaa atataatatg ttttatctgc
    19021 aaccataagg aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat
    19081 aagattatcg aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat
    19141 tataatcgcg atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag
    19201 ctggccgctg agcaaggtca ttgtgaagca caatactcct tagggcaaaa gtataccgag
    19261 gataaaagcc gtcataaaga taatgagcaa gccatcttct ggctgaaaaa agctgcccta
    19321 caaggccata ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc
    19381 aactataaag aagcggttgc ctggtatcag atagccgcgg agagcggaat gtcttatgcg
    19441 caaaataatc ttgggtggat gtacagaaat ggcaacggag tcgcacaaga ctatacgctg
    19501 gcatttttct ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg
    19561 gccgatcttt atgaagacgg aaaaggcgtt gctcaaaacg agacactcgc cgcattctgg
    19621 tatttgaaaa gcgcacagca gggtaatcgg catgctcagt ttcaaattgc atgggattat
    19681 aacgctggcg aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct
    19741 gccgctcagg ggagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa
    19801 ggcgttgaga aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat
    19861 gacgccactg cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc
    19921 gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc
    19981 agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat
    20041 ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg
    20101 gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta
    20161 gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc
    20221 gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt
    20281 tttatcggtg atgtctgcca gctcctgcca caagctttct ttaatcaccc gcataaagac
    20341 gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg
    20401 tctgacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac
    20461 gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc
    20521 gctcagtgac gcaaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc
    20581 cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc
    20641 acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga
    20701 aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctctaa
    20761 cgctactttt gtagccgtaa tgaaaacagg accactggct gccatgcaag agcaataccg
    20821 cccggaagag atagaatcca aagtacagct tcattgggat gagaagcgca catttgaagt
    20881 aaccgaagac gagagcaaag agaagtatta ctgcctgtct atgcttccct atccttctgg
    20941 tcgactacac atgggccacg tacgtaacta caccatcggt gacgtgatcg cccgctacca
    21001 gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg gacgcgtttg gtctgcctgc
    21061 ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg tggacgtacg acaacatcgc
    21121 gtatatgaaa aaccagctca aaatgctggg ctttggttat gactggagcc gcgagctggc
    21181 aacctgtacg ccggaatact accgttggga acagaaattc ttcaccgagc tgtataaaaa
    21241 aggcctggta tataagaaga cttctgcggt caactggtgc ccgaacgacc agaccgtact
    21301 ggcgaacgaa caagttatcg acggctgctg ctggcgctgc gataccaaag ttgaacgtaa
    21361 agagatcccg cagtggttta tcaaaatcac tgcttacgct gacgagctgc tcaacgatct
    21421 ggataaactg gatcactggc cagacaccgt taaaaccatg cagcgtaact ggatcggtcg
    21481 ttccgaaggc gtggagatca ccttcaacgt taacgactat gacaacacgc tgaccgttta
    21541 cactacccgc ccggacacct ttatgggttg tacctacctg gcggtagctg cgggtcatcc
    21601 gctggcgcag aaagcggcgg aaaataatcc tgaactggcg gcctttattg acgaatgccg
    21661 taacaccaaa gttgccgaag ctgaaatggc gacgatggag aaaaaaggcg tcgatactgg
    21721 ctttaaagcg gttcacccat taacgggcga agaaattccc gtttgggcag caaacttcgt
    21781 attgatggag tacggcacgg gcgcagttat ggcggttccg gggcacgacc agcgtgacta
    21841 cgagtttgcc tctaaatacg gcctgaacat caaaccggtt atcctggcag ctgacggctc
    21901 tgagccagat ctttctcagc aagccctgac tgaaaaaggc gtgctgttca actctggcga
    21961 gttcaacggt cttgaccatg aagcggcctt caacgccatc gccgataaac tgactgcgat
    22021 gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac tggggggttt cccgtcagcg
    22081 ttactggggc gcgccgattc cgatggtgac gctggaagac ggtaccgtaa tgccgacccc
    22141 ggacgaccag ctgccggtga tcctgccgga agatgtggta atggacggca ttaccagccc
    22201 gattaaagca gatccggagt gggcgaaaac taccgttaac ggtatgccag cactgcgtga
    22261 aaccgacact ttcgacacct ttatggagtc ctcctggtac tatgcacgct acacttgccc
    22321 gcagtacaaa gaaggtatgc tggattccga agcggctaac tactggctgc cggtggatat
    22381 ctacattggt ggtatcgaac acgccattat gcacttgctc tacttccgct tcttccacaa
    22441 actgatgcgt gatgcaggca tggtgaactc tgacgaacca gcgaaacagt tgctgtgtca
    22501 gggtatggtg ctggcagatg ccttctacta tgttggcgaa aacggcgaac gtaactgggt
    22561 ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc cgtatcgtga aagcgaaaga
    22621 tgcggcaggc catgaactgg tttataccgg catgagcaaa atgtccaagt cgaagaacaa
    22681 cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg gacaccgttc gtctgtttat
    22741 gatgtttgct tctccggctg atatgactct cgaatggcag gaatccggcg tggaaggggc
    22801 taaccgcttc ctgaaacgtg tctggaaact ggtgtacgag catactgcga aaggtgatgt
    22861 tgcggcactg aacgttgatg cgctgactga agatcagaaa gcgctgcgtc gcgatgtgca
    22921 taaaacgatc gctaaagtga ccgatgatat cggccgtcgt cagaccttca acaccgcaat
    22981 tgcggcgatt atggagctga tgaacaaact ggcgaaagca ccgaccgata gcgagcagga
    23041 tcgcgccctg atgcaggaag cgctgctggc cgttgtccgt atgcttaacc cgttcacccc
    23101 gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa ggcgatatcg acaacgcgcc
    23161 gtggccggtt gctgacgaaa aagcgatggt ggaagactcc acgctggtcg tggtgcaggt
    23221 taacggtaaa gtccgtgcca aaatcaccgt tccggtggac gcaacggaag aacaggttcg
    23281 cgaacgtgct ggccaggaac atctggtagc aaaatatctt gatggcgtta ctgtacgtaa
    23341 agtgatttac gtaccaggta aactcctcaa tctggtcgtt ggctaagcgc gggaggaagc
    23401 gtgcgatatc tggcaacatt gttgttatct ctggcggtgt taatcaccgc cgggtgtggc
    23461 tggcatctgc gtgataccac gcaggttcct tccactatga aggtcatgat cctggactca
    23521 ggtgatccga acgggccatt aagccgtgcg gtgcgtaacc agttacgtct gaatggtgtc
    23581 gagttgcttg ataaagaaac cacgcgtaag gacgttccat ccctgcgttt aggtaaagtg
    23641 agcatcgcga aagataccgc atcggtattc cgtaacggtc aaacagcaga gtatcagatg
    23701 atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg acatctaccc gattagcgcc
    23761 aaggtcttcc gttcgttctt cgataacccg caaatggcgt tagcgaaaga taacgaacaa
    23821 gacatgatcg taaaagagat gtacgaccgt gctgccgaac agctgattcg taagctgcca
    23881 agcatccgtg ctgcggatat tcgttccgac gaagaacaga cgtcgactac aacggatact
    23941 ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact gatgattcgg ttgtacccgg
    24001 aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc gtatctttta cttggtaacg
    24061 atcctctgtt attgcaggaa agccaggacg ctgttcgtca ggtagctgcg gcacaaggat
    24121 tcgaagaaca ccacactttt tccattgatc ccaacactga ctggaatgcg atcttttcgt
    24181 tatgccaggc catgagtctg tttgccagtc gacaaacgct attgctgttg ttaccagaaa
    24241 acggaccgaa cgcggcgatc aatgaacaac ttctcacact caccggactt ctgcatgacg
    24301 acctgctgtt gatcgtccgc ggtaataaat taagcaaagc gcaagaaaat gccgcctggt
    24361 ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca gacaccggag caggctcagc
    24421 ttccccgctg ggttgctgcg cgcgcaaaac agctcaactt agaactggat gacgcggcga
    24481 atcaggtgct ctgctactgt tatgaaggta acctgctggc gctggcccag gcactggagc
    24541 gtttatcgct gctctggcca gacggcaaac tgacattacc gcgcgttgaa caggcggtga
    24601 atgatgccgc gcatttcacc ccttttcatt gggttgatgc tttgttgatg ggaaaaagta
    24661 agcgcgcgtt gcatattctt cagcaactgc gtctggaagg cagcgagccg gttattttgt
    24721 tgcgcacatt acaacgtgaa ctgttgttac tggttaacct gaaacgccag tctgcccata
    24781 cgccactgcg tgcgttgttt gataagcatc gggtatggca gaaccgccgg ggcatgatgg
    24841 gcgaggcgtt aaatcgctta agtcagccgc agttacgtca ggccgtgcaa ctcctgacac
    24901 gaacggaact caccctcaaa caagattacg gtcagtcagt gtgggcagag ctggaagggt
    24961 tatctcttct gttgtgccat aaacccctgg cggacgtatt tatcgacggt tgatatgaaa
    25021 tctttacagg ctctgtttgg cggcaccttt gatccggtgc actatggtca tctaaaaccc
    25081 gtggaaacgc tggcgaattt gattggtctg acgcgagtca caatcatccc taataatgtt
    25141 cctccgcatc gtccccagcc ggaagcgaac agcgtacagc gtaaacacat gcttgaactg
    25201 gcgattgccg acaagccatt atttactctt gatgaacgcg agctaaagcg caatgccccc
    25261 tcttacactg cgcaaacact gaaagagtgg cggcaggaac aagggccgga cgtgccgctg
    25321 gcgtttatta ttggtcagga ttcattgctg acctttccga cctggtacga atacgaaacg
    25381 atactcgaca atgcacattt gatcgtctgt cggcgtccag gttacccact tgaaatggcg
    25441 caaccgcaat accagcaatg gctggaagat catttgacac ataacccgga agatcttcac
    25501 cttcagcctg ccggtaaaat ttatctggct gaaacgccgt ggtttaacat ctcggcgacc
    25561 atcatccgcg aacgtttgca aaacggtgaa tcatgtgatg atttattgcc ggaaccggta
    25621 ctgacttaca ttaaccaaca aggcttgtat cgctgatacc tgtcgttacg gagggcatga
    25681 tgcgactgtg gttaattcgt catggcgaaa cacaagcgaa tgtcgatggt ctgtacagcg
    25741 gtcatgcgcc cacacccctg accgcacgcg gtattgagca agcgcaaaat ctgcatacac
    25801 tgctagatga tgtttccttt gacctggttt tatgcagtga actggaacgg gcacagcata
    25861 ccgcgcgact ggttctcagt gaccgccagc tccccgttca tatcatccct gaactcaacg
    25921 aaatgttttt tggcgactgg gagatgcgac atcatcgcga cctcatgcaa gaagatgccg
    25981 aaaactatag cgcgtggtgc aatgactggc agcatgctat ccccacgaac ggtgaaggat
    26041 ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag gcttagtgaa tatcagcact
    26101 atcagaatat tttaatcgtc agccatcagg gcgtactgag tctgttaatt gctcgtttaa
    26161 ttggcatgcc tgccgagtct atgtggcatt ttcgcgttga ccaggggtgt tggagcgcca
    26221 ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg
    26281 agaatgcatg acttttctgt ttttttacgg gtaagccgca acgaccattg acagccccgg
    26341 gcaggctgat attctccgca gccagacttt ttccgccaga cacgactttg tagaaattgt
    26401 tttacaaaaa tggcgatgca atctgcggcg cggggtggga tgatagccca ctttcgaaag
    26461 ccgattcggc gacaattgtc ccgaaatcgc ctctggttca ggtatactga cagaccattt
    26521 ttatctattt gattcaccca gggggaaaac ttgcagggta aagcactcca ggattttgtt
    26581 atcgacaaaa ttgatgacct caaaggtcag gacatcatcg ccttagacgt tcagggcaaa
    26641 tccagcatca ccgactgcat gatcatctgt acgggtacgt ccagccgtca tgttatgtcc
    26701 attgctgacc acgttgtgca ggagtctcgc gcagcgggcc tgttaccgct cggcgtagaa
    26761 ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg
    26821 caggaagaga gccgtcgcct gtatgaactg gaaaaactct ggagttaatg cgtgaagctg
    26881 caacttgtcg ccgtaggaac gaaaatgccg gactgggtac aaaccggttt taccgagtac
    26941 ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg aaattccggc cggaaaacgc
    27001 ggcaagaatg cggacatcaa gcgcatactc gacaaagagg gtgagcagat gttggcggcc
    27061 gcaggcaaaa accgcattgt caccctcgat attccaggca agccctggga tacgccgcag
    27121 ttagccgctg agctggaacg ctggaagctg gatggtcgcg acgtcagtct actgattggc
    27181 gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg agcagagctg gtcgctgtcg
    27241 gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg cagagagtct gtaccgggcg
    27301 tggagcatca ccaccaacca tccttatcac cgtgagtgat aagggagctt tgagtagaaa
    27361 acgcagcgga tgaaactaca gaactctttt cgcgactata cggcagagtc cgcgctgttt
    27421 gtgcgccggg cgctggtcgc ctttttgggg attttgctgc tgaccggcgt gcttatcgcc
    27481 aacctgtata atctgcaaat tgttcgcttt accgactacc agacccgctc taatgaaaac
    27541 cgcattaagc tggtgcctat cgcgcccagc cgcggcatta tctatgaccg taacggtatc
    27601 cctctggccc tcaaccgcac tatctaccag atagaaatga tgccggagaa agtcgataac
    27661 gtgcagcaaa cgctggacgc tttgcgcagc gtggtagatc tgaccgatga cgatattgct
    27721 gcattccgaa aagagcgcgc acgttcacac cgtttcacct ctattccggt gaaaactaac
    27781 ctgaccgaag tacaagtagc tcgctttgcc gtcaatcagt accgttttcc gggtgtcgaa
    27841 gttaaaggct ataaacgtcg ttactatcct tacggttcgg cgttgaccca cgtcatcggc
    27901 tatgtgtcga aaatcaacga taaagacgtc gaacgcctga ataatgacgg caaactggcc
    27961 aactatgcgg caacgcatga tatcggtaag ctgggcattg agcgttacta tgaagacgtg
    28021 ctgcacggtc agaccggtta tgaagaggtt gaagttaaca accgtgggcg tgttatccgc
    28081 cagttaaaag aagtaccacc gcaagccgga cacgatattt acctgacgct ggatctcaaa
    28141 ctccagcaat atattgaaac gctgctggcg ggtagccgcg cagctgtggt agtcaccgat
    28201 ccgcgtacag gtggggtgct ggcgctggtt tccacgccta gttatgaccc aaacctgttt
    28261 gttgacggta tctccagcaa agattattcc gccttgctga atgacccgaa tacaccgctg
    28321 gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta cagtgaaacc ctatgtggcg
    28381 gtttcggcat tgagcgccgg ggtgatcacg cgcaatacga cgctgtttga cccaggctgg
    28441 tggcaactac cagggtcgga aaaacgttat cgtgactgga aaaaatgggg ccacggacgt
    28501 ctgaatgtca caagatcgct ggaagaatct gcggatacct tcttctatca ggtggcctac
    28561 gatatgggga tcgatcgcct ctccgaatgg atgggtaaat tcggttatgg tcattacacc
    28621 ggtatcgacc tggcggaaga acgttccggc aacatgccta cccgcgaatg gaaacagaaa
    28681 cgctttaaaa aaccgtggta tcagggtgac accattccgg ttggtatcgg tcagggttac
    28741 tggacagcga ccccaatcca gatgagtaag gcactgatga tcctgattaa tgacggtatc
    28801 gtgaaggttc cgcatttgct gatgagcacc gccgaagacg gcaaacaggt gccatgggta
    28861 caaccgcatg aaccgcccgt cggcgatatt cattccggtt actgggagct ggcgaaagac
    28921 ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc ataaatactt tgccagcgca
    28981 ccgtacaaaa ttgcggcgaa atccggtacc gctcaggtct tcggtctgaa agcgaacgaa
    29041 acctataatg cgcacaaaat tgccgagcgt ttacgtgacc acaaactgat gaccgccttt
    29101 gcgccataca acaatccgca agtggctgtc gccatgattc tggaaaacgg cggcgcgggt
    29161 ccggcggttg gtacgctgat gcgccagatc ctcgaccaca ttatgctggg tgataacaac
    29221 accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg aggaccatta atcatgacgg
    29281 ataatccgaa taaaaaaaca ttctgggata aagtccatct cgatcccaca atgctgctga
    29341 ttttactggc attgctggtt tacagcgccc tggttatctg gagcgccagc ggtcaggata
    29401 ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg tctggtcatc atggtggtga
    29461 tggcgcaaat tcctccacgc gtttatgaag gctgggcccc ctatctctat atcatctgta
    29521 ttattttgct ggtggcggta gatgctttcg gtgccatctc taaaggcgct caacgctggc
    29581 tggacctcgg tattgttcgt tttcagccgt cggaaattgc caaaatagcc gtaccactga
    29641 tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc gttgaagaac actggcatcg
    29701 cgctggtgct gatatttatg cccacgctgc tggtggctgc acagcctgac ctgggaacat
    29761 caatcctcgt tgcgctttcc ggtctgtttg tactgttcct ctctggcctt agctggcgtc
    29821 tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc gattctgtgg ttcttcctga
    29881 tgcatgatta ccagcgccag cgcgtaatga tgctcctgga cccggaatca gacccactcg
    29941 gcgcgggcta tcacattatt cagtctaaaa ttgctattgg ctccggcgga ttacgcggca
    30001 aaggctggct gcacggcact cagtcacagc ttgaatttct ccccgaacgc catactgact
    30061 ttatcttcgc ggtactggcg gaagagctgg gattagtggg cattctgatt ctgctcgctc
    30121 tctacattct gctgatcatg cgcgggctgt ggatagccgc cagagcgcaa accacctttg
    30181 gtcgcgtcat ggctggtggc ttaatgctga tattattcgt ttatgtcttc gtaaatattg
    30241 gtatggtaag cggtattctg ccggttgtag gggttccgct cccactggtc agttatggag
    30301 gatcggcgct aattgtgctg atggctgggt tcgggattgt aatgtcaatc cacacccaca
    30361 ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc
    30421 tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg atggtcagca acagacggta
    30481 agtgtaccgc agcctgcggt atgtaacggc cccatagttg aaattagcgg ggcggacccg
    30541 cgtttcgaac cactgagcgc gacggcaaat caggattacc agcgcgacgg taaaagctac
    30601 aaaatcgtgc aggatccgtc tcgatttagc caggcgggac tggcggcaat ctatgatgcc
    30661 gaaccaggca gtaacctgac ggcctctggc gaagctttcg atccgacaca gctgacggcg
    30721 gcccatccaa cgcttccgat ccccagctac gccagaatca ctaacctggc taacgggcga
    30781 atgatcgtgg tgcgcattaa tgatcgcggt ccttacggca acgaccgcgt tatttcgctt
    30841 tctcgcgcag cagctgaccg tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg
    30901 attattgtcg cccaggatgg ttcgctttct ggtcctggta tggcttgtac cacagtcgcc
    30961 aaacagactt acgccctgcc tgcacctccc gatttaagcg gtggcgcggg aacaagttca
    31021 gtgtctggcc cgcagggtga cattcttccg gtcagtaatt cgacgctaaa aagcgaagat
    31081 ccgaccggcg cgccggtaac cagcagcggt ttcctcggcg caccaacgac cttagcgcct
    31141 ggtgtactgg aaggcagcga accgacgcct gcgccacagc ccgttgttac agctccgtcg
    31201 acaacgcctg caacctcgcc tgcaatggtg acaccgcaag ccgcctcgca aagcgccagc
    31261 ggcaacttta tggtgcaggt cggggccgtt agcgatcagg ctcgtgcgca acagtaccaa
    31321 cagcaactga gacagaagtt cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcagtctgg
    31381 cggatccagc ttggcccatt cgccagcaaa gcagaagcca gtaccttgca gcaacgtttg
    31441 caaaccgaag cccaattaca gtcatttatt accaccgcgc agtagcgtaa agcaggcatc
    31501 tgatgtgtca atttctgtaa atgacgttaa caaagtcacg cggaaagtca gatgcctgcc
    31561 ggtagtgcat ttgctatagt agggcacttt ttttaattcc atcacggatg tcgtagttca
    31621 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    31681 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    31741 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    31801 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    31861 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    31921 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    31981 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    32041 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg atgcttttgt
    32101 tggcttgatg aacagctacg ttaacgccct gggcctgaaa aacacccact tccagacggt
    32161 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    32221 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    32281 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    32341 cggcatcaaa accggacaca ctgacaaagc aggttacaac cttgttgctt ctgcgactga
    32401 aggccagatg cgcttgattt ctgcggtgat gggcggacgt acttttaaag gccgtgaagc
    32461 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttt gaaaccgtta acccactgaa
    32521 agtaggtaaa gagttcgcct ctgaaccggt ttggtttggt gattctgatc gcgcttcgtt
    32581 aggggttgat aaagacgtgt acctgaccat tccgcgtggc cgcatgaaag atctgaaagc
    32641 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    32701 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    32761 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    32821 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    32881 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    32941 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    33001 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    33061 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    33121 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    33181 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg cacctggtcg
    33241 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    33301 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    33361 gccaatctcc caggctatgc atgaattcac cgatacccgc gatgatagta cccttgatga
    33421 aatctggctg gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca
    33481 cattttaatg cctggtgata ttccggtgat tcagagcgat cgcggtgggc aggtgactta
    33541 tcacgggccg gggcaacagg tgatgtatgt attgcttaac ctgaaacgcc gtaaactcgg
    33601 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    33661 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gttggggaaa agaaaatttg
    33721 ctcactgggt ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    33781 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    33841 taaaatatca caatggaaac ccgaagcgac gactaataat attgcaccac gtttactgga
    33901 aaatatttta gcgctactaa acaatcctga cttcgaatat attaccgctt aatttcatac
    33961 ctcaatggcc caatttacac tgggtcatta ctcataacac ctgctaatgc tttacatctc
    34021 cttcgcgttt gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt
    34081 aactcacccc attgattaac ttattgaaat agttgattat gatgagcaaa agcgttccgt
    34141 tccaattaga cataagatta ttgtaatttt tttaaatatt atttttccat gaataaaatt
    34201 ggagtaagtg cgtggatagt aataatcaaa tcgaaccctg tttaagcaga aagtcgtcag
    34261 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt
    34321 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    34381 cctcggcaat cagtcagtct atacagaaac tgcgcgttat attccctgac ccattgttta
    34441 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    34501 gtcagggcct tgaatccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    34561 gaacgataac tatcgcgacc accccctcgg tcggagccct ggtccttcct gtcatttatc
    34621 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    34681 aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc
    34741 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcgagggaa
    34801 atccactact ctctttagaa gatgacagag agactatcga caacgctgca catgtactcc
    34861 tgttaccgga aggacaaaat ttcagcggtc tgcggcagag aattcaagag atgtttccgg
    34921 accggcagat cagcttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    34981 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagccgt tgctggccgc
    35041 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    35101 acaaattcag cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    35161 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    35221 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    35281 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    35341 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    35401 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    35461 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    35521 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    35581 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    35641 ctgggcgcta tttgtacccg ccgttgtccg ttctgtgacg ttgcccacgg tcgcccggta
    35701 gctcctgatg ccaatgagcc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    35761 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt
    35821 gcggattgta ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    35881 ccggatttcc gcggtcgtat ggatcgtgct ctggatattc tgactgcaac gcccccagat
    35941 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca
    36001 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    36061 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
    36121 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    36181 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    36241 gcagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    36301 cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa ttacttacat
    36361 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    36421 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    36481 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    36541 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    36601 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    36661 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
    36721 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    36781 ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgca cctgacgcac
    36841 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    36901 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
    36961 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtgatg
    37021 ctctttgagc ggcccgcgaa cccaggcggc aggaagtgcc aggatttcag ctccctgtaa
    37081 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    37141 catcccttcc acctccagta acggagcgat ttcattacca gcatcaacac ggcgtgattc
    37201 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacaa tgtttcctgc
    37261 ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt gaaggaacat gaatcgtcag
    37321 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    37381 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgtg ccagcaatgc
    37441 ttccggcagg acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    37501 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat
    37561 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    37621 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    37681 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
    37741 atcaggcgat tccgttgggg acgctggctg caaatctgat tggggcattc atcataggaa
    37801 tgggattcgc ctggttcagc aggatgacaa acattgatcc cgtgtggaaa gtattaatca
    37861 ccaccggatt ttgtggcggt cttacaacct tctcaacatt ttcggcagaa gtggtgtttt
    37921 tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaac cttctggggt
    37981 cttttgccat gaccgcactg gcattctggc tgttttcggc ctcaaccgca cactaaagac
    38041 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    38101 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    38161 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    38221 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    38281 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    38341 cggttaccag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    38401 aaaagtcgcg ttatttttga tgttaattat tttagttatt gattttaaag aagttactaa
    38461 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    38521 aggtacccgg aatgtaggtc atctgaaaag tcactgggcc ataacccacg gaggccaatg
    38581 gcagtagaac cgggagaggg atgtaattcc agttatcacg tgccgttacg ccagcggtga
    38641 atcccagacc taaatgaaaa ttttcattcg ccagcggtcg ccaggtactt tcccatccgt
    38701 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    38761 gccagtttcc tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt
    38821 tatagcgatc ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa
    38881 tatataaatc ataatgttca ggctgttgcc aggtttgtgc aatattttct ctaaacgttg
    38941 tcatccactc atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa
    39001 aggaaaagat agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa
    39061 atctactact agcatagcaa agctacaatt aacataacct taatagcccc aacataaaga
    39121 ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc
    39181 gattaaagat gattcgtcgt tttctgcgat gggaatagtc ataaaagaaa aagcaagttg
    39241 tttctgataa caaaatgcaa ccaagagaaa attactttat tttttaacag catacaaaaa
    39301 gcatcataac gaagaatata ttatatatcg tcaacccacg cccattgcag attatttttt
    39361 gagcacacaa ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    39421 tttacacaca ttattatgcg ataattgcca aacgctaaat atgcgttttg ttatctatgt
    39481 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcac
    39541 cgccaattta ttgggcatat tttttcctta agttttagga attttttatt atttactttg
    39601 gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt
    39661 tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggcg tgttatttgt
    39721 cggtggcctg ttattgctga ttattagtgc cattatgggg cacaaagtgt taccgtccag
    39781 ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa tattgctaat
    39841 gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggctttg ccgcttacat
    39901 gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tacagtatat
    39961 taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga tgtctctggc
    40021 cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctgtttc cggtgatggt
    40081 aaacgttggt atcagtcgtg gagcagctgc tgccatttgt gcctccccgg cggcgattat
    40141 tctcgcaccg acttcagggg atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat
    40201 tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat
    40261 cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatatctc atgaaatgtt
    40321 agatgtcagt gaaataacca ccactgcccc tgcgttttat gccattttgc cgttcacgcc
    40381 gatcatcggg gtgctgattt ttgacggcaa atggggtccg caattacaca tcatcactat
    40441 tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca
    40501 gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcat ttgcaaacgt
    40561 ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat
    40621 tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt
    40681 attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta
    40741 tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt
    40801 gactatcccg atgctgcagg cgtcaaacct cggtcgtacc ctgtcacccg tttctggcgt
    40861 agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc
    40921 ggtgccagtg cttgttgggc tggtgattgt tatcgttgct acagagctga tggtgccagg
    40981 aacggcagca gcggtcacag gcaagtaaat agtcatgccg gaggcagttt cctccggctt
    41041 tatcatgtca cccactgtgg tatatgcgtt gcggtctgcc aactttgccg tgaacaattt
    41101 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    41161 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacacccggc tctttaaaca
    41221 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggtagctcg tccttaggtt
    41281 caccgcgagc ataagcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    41341 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagc cgttcatagg
    41401 caattggctt aatgagataa tcaaatacac cacaacgtac ggcttcagac accgtttcca
    41461 tatcgctggc tgcagtggta aacaccacgt cgccgggata atgcgcctgc accagttcat
    41521 gcagtaaatt aatccctcta ccgtcaggaa gatagttatc gagcaagatt agccccggct
    41581 taaaacgctc gatcatcatt cgggcctgcg ccaggtttcc cgccagtaat atctgactga
    41641 atccgggaat gtgacgaata tattccgcat gcatctctgc cagcggcgtt tcgtcctcaa
    41701 cgatcaatag ggttaatgga gctgtcatta ggtttcactt tcggaatata gattgaaaat
    41761 aaggtaccgc agggatcatt atcttcgaga gtgataacac caccgcagcg cgttacgtag
    41821 ctggcaatca agtacaaccc aatgccatgt tcaccgggct cgtcagcacg cgtactgacc
    41881 ccctgctcaa atattttgtc tcgtagagac tctggaacgc cgcagccctg atcggcgact
    41941 tcaatcacca catcatcgcc ttcatcgctg aggaataatt caacgatctt gtttccttca
    42001 tcgctacgca ggctggcttc gaaggcgtta tcaagtaaat tgccaacaat ggcggcaaac
    42061 tcggtgctat ccagtcctgg cggcagttgc gaaagctggc taccggggac gatgatcatt
    42121 tttagcccca gttcgcgggc gcgctgcact ttaccaaaaa gcagccccgc cacctggcga
    42181 tcggcaaacg cctcgcgcag gctgtcaata agctgttgct gggcctgaga ctccccctgc
    42241 accatcgcca gcacgcgatc atactctttc atctgcaaca gaccattgag cgtcgacatc
    42301 caattgagat gctcgtgtcg caatgtacga aggctctcga cgtattgttt tatttgcgtc
    42361 agttgcgcat tgagggtgga aatttcgtct ttactacgaa agctgataat ggcccccagc
    42421 aaatcatcac ctgaacgaat agcttcccgg ttggcaataa cgctcagacc gttaaagttc
    42481 gccaccacat cctgacgttt ttcatcaatc tgttcggtaa agaaatcggc aggcctgacc
    42541 acttctgcaa tgggtttacc caaccattgc cgtccagggg aactcagccc cagcatcttt
    42601 cttgcgttac gattgatggc ggtaatgtaa ccgtgcggat ccaccgcaat cagcccttca
    42661 taaaccgaac taaacagcgc ctcttgctga cggaccacgc gtgcgatttg ctttggctcc
    42721 atgcccatca tctgccgacg gatatgcgcg gccaggaacc acgacagcaa catcagaatc
    42781 cctaacagca cgacaaacac gcctgccatc ggtaataaaa actcagcccg ccagctatcg
    42841 attttactca ccaggtagcc aatcgacacc acgccgatga cttttccatc gtcatcaaag
    42901 attggcgttt tggcgcgcat cgccataccc attgaccctt tcccggtaat gaagtagctc
    42961 tcccctttct ccagcgcgcc ctgtttggtg aactgcatag gatgaccaat tttctccgga
    43021 ttaggatggt aaaggcggat cgagtgccgg tcgccaatca ccacataatc aaaatcggta
    43081 tctctttgta atttgttagc gatggtcgcc agccgtttgt agtcacgcgt ttttactgcg
    43141 ctgatgatac tgtcattaga agcaataatt ttcgcctgat tcattgccat gtcgcgtaca
    43201 tgaagcgtta aatagtcctc aaaactggcc gtaaaatatt gcgccagggc tgcaatgaca
    43261 aatattgaga acaccagaat cagcaaaaag atacgcagcg gaaatgccag tctttggaaa
    43321 aatgcaaact gtttattctc gttaagctgc aacattgttt tccttgcggg ttgtcccgag
    43381 cctctttgac g
//
LOCUS       DAZGCQ010000034        42891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000034 DAZGCQ010000000
VERSION     DAZGCQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.034"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..164
                     /locus_tag="ACHK07_001977"
     CDS             <1..164
                     /locus_tag="ACHK07_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8102310.1"
                     /translation="PYELYNSHRDFLNLKEIKLEHPLPSIKLYISYNKSSLNNLVFSR
                     FIDRLNECF"
     gene            311..1531
                     /gene="ybdN"
                     /locus_tag="ACHK07_001978"
     CDS             311..1531
                     /gene="ybdN"
                     /locus_tag="ACHK07_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8102311.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1504..2133
                     /gene="ybdM"
                     /locus_tag="ACHK07_001979"
     CDS             1504..2133
                     /gene="ybdM"
                     /locus_tag="ACHK07_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8102312.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2134..3294)
                     /gene="ybdL"
                     /locus_tag="ACHK07_001980"
     CDS             complement(2134..3294)
                     /gene="ybdL"
                     /locus_tag="ACHK07_001980"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO8102313.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3403..4491
                     /gene="hcxA"
                     /locus_tag="ACHK07_001981"
     CDS             3403..4491
                     /gene="hcxA"
                     /locus_tag="ACHK07_001981"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO8102314.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNNAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4501..4698)
                     /gene="ybdD"
                     /locus_tag="ACHK07_001982"
     CDS             complement(4501..4698)
                     /gene="ybdD"
                     /locus_tag="ACHK07_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8102315.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4880..6985)
                     /gene="cstA"
                     /locus_tag="ACHK07_001983"
     CDS             complement(4880..6985)
                     /gene="cstA"
                     /locus_tag="ACHK07_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO8102316.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKEMP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7166..7579)
                     /gene="entH"
                     /locus_tag="ACHK07_001984"
     CDS             complement(7166..7579)
                     /gene="entH"
                     /locus_tag="ACHK07_001984"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO8102317.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7582..8328)
                     /gene="entA"
                     /locus_tag="ACHK07_001985"
     CDS             complement(7582..8328)
                     /gene="entA"
                     /locus_tag="ACHK07_001985"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO8102318.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8328..9185)
                     /gene="entB"
                     /locus_tag="ACHK07_001986"
     CDS             complement(8328..9185)
                     /gene="entB"
                     /locus_tag="ACHK07_001986"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO8102319.1"
                     /translation="MAIPKLQAYALPEAHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9199..10809)
                     /gene="entE"
                     /locus_tag="ACHK07_001987"
     CDS             complement(9199..10809)
                     /gene="entE"
                     /locus_tag="ACHK07_001987"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO8102320.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAANNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFIAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10819..11994)
                     /gene="entC"
                     /locus_tag="ACHK07_001988"
     CDS             complement(10819..11994)
                     /gene="entC"
                     /locus_tag="ACHK07_001988"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO8102321.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12183..13139
                     /gene="fepB"
                     /locus_tag="ACHK07_001989"
     CDS             12183..13139
                     /gene="fepB"
                     /locus_tag="ACHK07_001989"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8102322.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGIHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13143..14393)
                     /gene="entS"
                     /locus_tag="ACHK07_001990"
     CDS             complement(13143..14393)
                     /gene="entS"
                     /locus_tag="ACHK07_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO8102323.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14504..15508
                     /gene="fepD"
                     /locus_tag="ACHK07_001991"
     CDS             14504..15508
                     /gene="fepD"
                     /locus_tag="ACHK07_001991"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO8102324.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15505..16497
                     /gene="fepG"
                     /locus_tag="ACHK07_001992"
     CDS             15505..16497
                     /gene="fepG"
                     /locus_tag="ACHK07_001992"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO8102325.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16494..17309
                     /gene="fepC"
                     /locus_tag="ACHK07_001993"
     CDS             16494..17309
                     /gene="fepC"
                     /locus_tag="ACHK07_001993"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8102326.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17306..18439)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK07_001994"
     CDS             complement(17306..18439)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK07_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO8102327.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18655..22536)
                     /gene="entF"
                     /locus_tag="ACHK07_001995"
     CDS             complement(18655..22536)
                     /gene="entF"
                     /locus_tag="ACHK07_001995"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO8102328.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAAQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHSERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFSSLLGCDVQDVDADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22533..22751)
                     /gene="ybdZ"
                     /locus_tag="ACHK07_001996"
     CDS             complement(22533..22751)
                     /gene="ybdZ"
                     /locus_tag="ACHK07_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO8102329.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22754..23956)
                     /gene="fes"
                     /locus_tag="ACHK07_001997"
     CDS             complement(22754..23956)
                     /gene="fes"
                     /locus_tag="ACHK07_001997"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO8102330.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLEAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24199..26462
                     /gene="fepA"
                     /locus_tag="ACHK07_001998"
                     /pseudo
     CDS             24199..26462
                     /gene="fepA"
                     /locus_tag="ACHK07_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            26637..27257
                     /gene="entD"
                     /locus_tag="ACHK07_001999"
     CDS             26637..27257
                     /gene="entD"
                     /locus_tag="ACHK07_001999"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO8102331.1"
                     /translation="MKTTHTSLPFAEHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27379..27531)
                     /gene="hokE"
                     /locus_tag="ACHK07_002000"
     CDS             complement(27379..27531)
                     /gene="hokE"
                     /locus_tag="ACHK07_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO8102332.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            27500..27768
                     /locus_tag="ACHK07_002001"
                     /pseudo
     CDS             27500..27768
                     /locus_tag="ACHK07_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27983..29101
                     /gene="ybdK"
                     /locus_tag="ACHK07_002002"
     CDS             27983..29101
                     /gene="ybdK"
                     /locus_tag="ACHK07_002002"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO8102333.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29167..29415
                     /gene="ybdJ"
                     /locus_tag="ACHK07_002003"
     CDS             29167..29415
                     /gene="ybdJ"
                     /locus_tag="ACHK07_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO8102334.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29480..29848
                     /gene="ybdF"
                     /locus_tag="ACHK07_002004"
     CDS             29480..29848
                     /gene="ybdF"
                     /locus_tag="ACHK07_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8102335.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29942..30595
                     /gene="nfsB"
                     /locus_tag="ACHK07_002005"
     CDS             29942..30595
                     /gene="nfsB"
                     /locus_tag="ACHK07_002005"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO8102336.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30853..32100
                     /gene="ybdG"
                     /locus_tag="ACHK07_002006"
     CDS             30853..32100
                     /gene="ybdG"
                     /locus_tag="ACHK07_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO8102337.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32168..33544)
                     /gene="pheP"
                     /locus_tag="ACHK07_002007"
     CDS             complement(32168..33544)
                     /gene="pheP"
                     /locus_tag="ACHK07_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO8102338.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTIDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33646..36789)
                     /gene="cusA"
                     /locus_tag="ACHK07_002008"
     CDS             complement(33646..36789)
                     /gene="cusA"
                     /locus_tag="ACHK07_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO8102339.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDTTLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36801..38024)
                     /gene="cusB"
                     /locus_tag="ACHK07_002009"
     CDS             complement(36801..38024)
                     /gene="cusB"
                     /locus_tag="ACHK07_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO8102340.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDSATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38040..38372)
                     /gene="cusF"
                     /locus_tag="ACHK07_002010"
     CDS             complement(38040..38372)
                     /gene="cusF"
                     /locus_tag="ACHK07_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO8102341.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38396..39778)
                     /gene="cusC"
                     /locus_tag="ACHK07_002011"
     CDS             complement(38396..39778)
                     /gene="cusC"
                     /locus_tag="ACHK07_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO8102342.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNGGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39935..40618
                     /gene="cusR"
                     /locus_tag="ACHK07_002012"
     CDS             39935..40618
                     /gene="cusR"
                     /locus_tag="ACHK07_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO8102343.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40608..42056
                     /gene="cusS"
                     /locus_tag="ACHK07_002013"
     CDS             40608..42056
                     /gene="cusS"
                     /locus_tag="ACHK07_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO8102344.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
     gene            42650..>42891
                     /locus_tag="ACHK07_002014"
     CDS             42650..>42891
                     /locus_tag="ACHK07_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8102345.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFEL"
BASE COUNT        10284 a        11817 c        10890 g         9900 t
ORIGIN      
        1 tcccctatga actttataac tcccaccgtg actttcttaa tttaaaagag ataaaactag
       61 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaacaatc
      121 tggtgttctc ccgatttatt gaccgtctaa atgagtgttt ttgatatata acctcagaca
      181 gctactataa ctttcaccta tttattcaca gcgcgaatga aaaataaaac actctgcttt
      241 tcagtgttta ctttataatt aacagtctta tctcactgat caccctgtaa cgttcagaga
      301 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
      361 agagcgtatt acctggacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
      421 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
      481 aatctgcgtt ttgtttatcg actgggaggc gcaattctct tgcaccatta actatgttca
      541 gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg cgctcccgct
      601 tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg aacctgatgt
      661 cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat cctgactttt tctgctttta
      721 ccagccaggc atgaccttcg aacaatttgt acgtgaattt gccgaatggt tttcacaaaa
      781 acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc gttttgtcgc
      841 catcgccagt ttaaataaac aacgttttgc cgacgataaa ccctggacta cggccgcacc
      901 aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg atatctggac
      961 ctggtatgca aatcatcaaa gtctctgcaa cccactgtat aacctaatgt atcaggcagg
     1021 cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac gacaaggatt
     1081 atggctctat cacgttatcg aaccggatcg ctgggctgct atgtgcgcac gagtcagcgg
     1141 ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc atcggaaaat
     1201 cctcaagcca gaacatttag actggcaaga atatgcatta ttgctgctca atagcatgcc
     1261 ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact ggtatcagaa
     1321 aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag atatcccctc
     1381 ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg cattatcatt
     1441 tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag gaaaacgtca
     1501 ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt tctcgccagc
     1561 ttgccggaag atgatcgcat caacgcgatt aatgaaatcc gcatggcgat ccatcaggtt
     1621 agcccctttc gtgaagagcc agtggattgc gttctgtggg ttaaaaacag ccaacttatg
     1681 cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct gaaaaaatcc
     1741 attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa aaatgccctg
     1801 gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc attaaaatta
     1861 cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca acgcatcgcc
     1921 gcgactattc gtcataatcg cgctcgtgga cgccatcaaa tcaccgccat gtcggaaatc
     1981 gtccgtgaac ttagccagtt gggatgggac gataataaaa ttggcaaaga gctgggaatg
     2041 gacagcgacg aagtgctgcg cctgaagcaa attaacggcc tgcaagagtt gtttgccgac
     2101 cgtcaatatt cccgcgcctg gacggttaaa tagctaaagc tggcgcaggc gttcagctgc
     2161 tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat gagggaaggg
     2221 atcggcgcaa aacaccgata gcggaatcgc cgctacgccg tgctcctgcg tcagccactg
     2281 gcaaaactca acatcatcca gggtagatac ggcgctgtaa tccaccagca aaaagtatgt
     2341 accttcacac ggtaaaatct ccagccggct ttcatttaaa gcattcacca gaatatcgcg
     2401 cttctggcga taaaagtccg gtaacgcaag ataatgctca ggttctgcac gtagcatatc
     2461 agcaagcgcc agctgtgccg gggtattcac cgaaaaggtc agatactgat gtaccttgcg
     2521 aatttcggca ctgatgggcg ctggcgcaac acaataaccc actttccagc cggtcatatg
     2581 ataggtcttg ccaaatgaag aaaccgccac tgcccgctca cgcagctgcg gatgcgccag
     2641 cacactggca tggccctgtt gtgaaaagtt gatgtgctcg tagacttcat cgctaatgac
     2701 aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg aaatcagcct gctgccagac
     2761 agttgcactg ggattatgcg gagtgttgag gatcaccagt ctggtgcgct cgcttaacaa
     2821 tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt tgcagtgcca tacgcttcac
     2881 tattccccca gaaagcgcga tggcgggggc gtaactgtca tagctgggat caaaacaaat
     2941 cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg tataacgctt ccgtcgcccc
     3001 tgccgttacg gtgatatcgc tatcggcatc tggttgatag ccatacaaac gttccgtttt
     3061 ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg ggcgcgtatt ggtttgcccc
     3121 ctgggcaacg tggtacgcca gccgctcctg taaatagcgc ggaccatcaa aatcaggaaa
     3181 gccttgcgac aggttaatcg cctggtgttg ctgcgccagc gcgctcatct gggtgaaaat
     3241 agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg tcatttttat
     3301 tgtacctgac gaagttgcgt gttgaaggca ctataacacg atgctagtat ttggcaatca
     3361 agacgtttag atgtctaaat aaaacaataa ggacaacaca acatgcctca caatcctatc
     3421 cgcgtggtcg tcggcccggc taactacttt tcacatccag gaagtttcaa tcacctgcac
     3481 gattttttca ctgatgaaca actttctcgc gcggtgtgga tctacggcaa acgcgccatt
     3541 gctgcggcgc aaaccaaact tccgccagcg tttggacttc caggggcaaa gcatattttg
     3601 tttcgtggtc attgcagcga aagcgatgta cagcaactgg cggctgagtc cggtgacgat
     3661 cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg acaccgcgaa agccctcgcc
     3721 cgccgtctcg gtctgccatt tgttgccgct ccgacgatcg ccgccacctg cgccgcctgg
     3781 acaccgctct ccgtctggta taacaatgcc ggacaggcgc tgcattatga gattttcgac
     3841 gacgccaatt ttatggtgct ggtggaacca gagattatcc tcaatgcgcc gcaacaatat
     3901 ctgctggcgg ggatcggtga cacgctggcg aaatggtatg aagcggtggt gctggctccg
     3961 caaccagaaa cgttgccgct aaccgtgcga ctggggatca ataatgcgca ggccattcgc
     4021 gacgtcttgt taaacagtag cgaacaggcg ctgagcgatc agcaaaatca acagttaacg
     4081 caatcatttt gcgatgtggt ggatgctatt attgctggtg gtgggatggt cggtggtctg
     4141 ggcgatcgtt ttacgcgtgt ggcggcggct catgccgtgc ataacggtct gaccgtgctg
     4201 ccgcaaaccg agaagtttct tcacggcacc aaagtcgcct acggaattct ggtgcaaagc
     4261 gccttgctgg gtcaggatga tgttctggcg caattaactg gagcgtatca gcgttttcat
     4321 ctgccgacta cgctggcgga gctggaagtg gatatcaata atcaggcgga gatcgacaaa
     4381 gtgattgccc acaccttgcg cccggtggag tccattcatt acctgccagt cacgctgaca
     4441 ccagatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt ttaaagcctg acgtgcaaat
     4501 ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg tcctgccgct cacggaaaaa
     4561 ctcctcgtag gtcatcggcg tttgatcggg atggttaacc cgcatatgtt cgacatagtt
     4621 gtcgtaatca ggcataccaa tcatcagttt cgccgcctga cctaaatatt ttccggcttt
     4681 tgccagtgaa tcaaacatct tggcctccgc taaccatgtt gctgaaagag tgccggatgc
     4741 ggcgtgaacg ccttatccgg cctacaaatg ctatcaacgc gctcggtcag tcccctcgcc
     4801 ccatcgggga gagggttagg gtgaggggga acccacggcg ccatgccaac attcgccaac
     4861 atcccccctc actctgactt tagtgcgcgc cttttgcctg cgccacgatc tcctcgacat
     4921 tttccggcat tggctcatac ggcatttctt tcgccgttgg cttcggctct ttcaatgccg
     4981 caagtgccgt cttaatcgag aacagtgcca gaaccaccac gaccaccata aagaagatgg
     5041 ttaacccggc atccagacgg ttgttgaaca ccagttgcgc cagctgtgac tcagtatact
     5101 gcgacggaat attgccgctg tcgatcattg cctggaactt gttagcaatg gccaggaagc
     5161 cgactttcgc atccgggcta aacgctttct gccagccagc ggtcagggta caaatcagca
     5221 gccaggccgt tggtaccagc gccacccagg cgtaacgttg acgtttcatc ttgaacagca
     5281 ccacggcaca gagcatcagc gccatccctg ccagcatctg gttggcaata ccaaacagcg
     5341 gccacagagt gttaataccg cccagcggat cgaccacgcc ctgatggagg aagtagcccc
     5401 acgctagcac gcacagcgcc gttgccagca ggttagcagg cagtgaatcg gtccgcttca
     5461 ggccaggaga caccacgccc agcagatcct gcaacataaa gcgcgcagca cgcgtacccg
     5521 catctaccgc cgtcagaata aacaatgctt caaacaaaat ggcgaagtga taccagaacg
     5581 ccacatccat catgccgccc agcgcgccgt gcagaatgta ggccatcccc accgccagcg
     5641 tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc cacttcgctg gcaatctggt
     5701 ttagcgtatc cggggtaatc gcaaagcccc agctactcac cacttgcgcc gcagaagcga
     5761 ccacatccgc cgtccctgcc ggagccagca ccgccatcgg gctgttcatg gcgaaataga
     5821 cgcccggatc gatgatacag gcggaaacca gtgccataat cgccacgaag gattccatca
     5881 acatcccgcc gtagccgata aagcacgcct gcccttcgtt cgccagcatc ttcggcgtgg
     5941 taccggaaga gatcagcgca tggaagccag acaccgcacc acaggcgatg gtgataaaca
     6001 ggaacgggaa caggttaccg gtccataccg ggccagtgcc atcgacaaat ttggtcagcg
     6061 caggcatggt cagcgtcgga cgcataatca aaatgcctac cgccagaccg acgatagtcc
     6121 cgattttcag gaaggtagag aggtagtcac gcggggccag cagtaaccac accggcagca
     6181 ccgccgccac aaaaccgtag cccaccagca tccaggtcag ctgcacgccg gtaaagtcaa
     6241 agtacggtgc ccaggtcggg ctttctgcca cccagccgcc agagataatg gcaaaaatca
     6301 ggaacaccag gccaatcact gacacttcgc caatacgccc cggacgcaga tagcgcaggt
     6361 agattcccat aaaaatcgcc agcggaatgg tgaacgcaac ggtatacgtt ccccacgggc
     6421 tatgagtcag ggctttcacc acgatcatcg ccagtactgc aaggataatg accatgatca
     6481 taaagcaggc caccagcgcg atcaccccgg cggttggccc catctcttct ttgaccagct
     6541 cacccagcga acgaccgtca cggcgcgtag aaacaaacag caccatgaaa tcctgcaccg
     6601 caccggcgag aaccaccccg gcgagtagcc agatcatccc tggcaggtaa cccatttgcg
     6661 ccgccagtac cggcccaacc aacggacctg ctccggcaat ggccgcaaaa tggtgaccga
     6721 acagcacttt cttgtccgtc ggcacatagt ccagcccgtc gttatggcgc accgctggcg
     6781 tcatacgcgt cgggtcaacc gccagcacat ttttggcgat atacaggcca taaaaacggt
     6841 aagcgatcag atagatacag accgacgcca ccacaatcca cagcgcgttg atctgttccc
     6901 cacgatttaa agcaatgtat cccagagcaa atgctcccat tacagagagc actgtccaga
     6961 cgaggtattt ccctgatttg ttcatagttg ttatccgtgt gcgtgtccat agagatgtta
     7021 cattttgttt ctataacatc tttgtacgat ttaacaacac ggcaaccatg taaatcggag
     7081 ttgaaaccaa tatttaactt aacaatgtta actcgatcac tccgttaacc gagttttcct
     7141 gcaatctcat ttaattctgt cgggctcatc ccaaaactgc cgtacccagc cgacaagtgc
     7201 aacaacgccg cccctgttca tcgaaaacga cgatttccca gctctgattt tgccgcccaa
     7261 gatgcagcgg ctggcagacg ccgcgtacct tgccttcaga caccgggcga tgatgggtgg
     7321 cgttaagttc cgtgcccacc acacactgcc cgtcacgggt catcataaat ccggccatcg
     7381 atcccagcgt ttcagccagc gcagccgacg cgccgccatg cagcaggcca aacggctgat
     7441 gagtacgggt atcaaccggc atttcggctt ccagcacatc atcgcccaga cgggtataca
     7501 caattcccag atgcgccacc attgtgttat cgctggtggc gttcagttcg tcgagcgtta
     7561 aatggcgttt ccagatcatg cttatgcccc cagcgttgag ccgccatcga ccacaatatc
     7621 ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac aaaatcgtgt tggcgatctc
     7681 ttgtggacgg gcgattttcc ccagcggaat gccgagttta aactgctcgc caaagccgcg
     7741 aatacgctgt tcttcggcgt catcgctcac ccacagcgtg cgttgcatat cggtatcggt
     7801 ggaacccggc gaaaccacat tacagcgcac gccgctaccc gccagttcca gcccgacgct
     7861 caacgccagg cttttcagcg ccgctttcga tgcgccataa gcactcatac caatacgcgg
     7921 cgtgtgtgcg gcgtcggacg ccacagtgac aatcgccccg ccccgctgac ggcgaaactg
     7981 gttcatggtt tgctggaaca ggttaaacgc accgccgacg ttaaccgcaa aagtctgctg
     8041 ccagtcctcc ttactgagct gatcggtcgc gcccatgcgc aaaattcccg ccgcattgac
     8101 cagcacgtcc agtcgctccg tttcagctaa cagtcgctga catacctgcg ccacctgcgc
     8161 agcatcggca acatccatta cttcggtcgc aaagggatat tgctcctgag cgaacgcttg
     8221 atcaaaacct gtaactttcg ctcccgcttc aacaaacgcc agcgccgtgg cgtagccgat
     8281 acctttcccc gcgccggtta cccagacatt tttaccgctg aaatccatta tttcacctcg
     8341 cgggagagta gcttccacca ggcgtcgatg gtcgggtttt tcgccaacat gacaaagtcg
     8401 atatcgccat gcactttgcg ccagcgcgcc gccagcgcca tcatgcgcac cgaatccaga
     8461 ccgtagtcga tcaggttgtc gtcatcgaac ggttcatcgg actcgtccag caacggcagg
     8521 atcacctcac gcagcgccgc tttgctggcg gggataggtg ctggcagtaa ttcttcggtc
     8581 atcaccaccc ggccagaacg tccggccaca tatttcagcg acatcaaatg ctcgtcacgg
     8641 ctgaaatcgg ccagcgcatc cgccaccata aacggtttaa tatcgcgcat aaatgcgtcg
     8701 gttgcggtgg tcatacagcc aatgtgggca tatacccctg taataatcag ctggttacgt
     8761 ccactctctt tgagcatttg ctccagcgga gaacgatgaa acgcgctgta gcgccacttc
     8821 accagcaccg tgtcgtcggc atctggcgtc aggcgatcca ccaccttttg ctgttccggc
     8881 gagcgggtca ggcccggccc ccacatatca ttcaacagcg cccgatcttc atcgctctgc
     8941 tctttcggct gggcggtgta ataaaccggg atattgtgct gtttgcagta gtcgcgcagc
     9001 gcagcaatat tcgcgatcac ctgctccatc atcgggcagt tctcgcccca gaagctgaca
     9061 aaatagtcct gcatatcatg gattaacaac gcggcacgtt gcggttcaaa ggcccagtcg
     9121 actttattct tagggatatc gtgagcctcc ggcagtgcgt aagcctgtaa ttttggaata
     9181 gccatcgtgt tctctccttc aggctgatgc gcgtgacgcc agccactgac gtaattgttt
     9241 tttatcgact ttcccgaccg ccgtcagcgg aagtgaatcc acacactcca cgcgatccgg
     9301 taatttaaat tcggcaatac cctgttcacg caggaaacga cgcacctgca ccgcgcgcag
     9361 cggctctttt accaccagat aagcacagct tttttcgccc atcagctcat cttccatact
     9421 gaccagtgcg gcgtagatca ccgccggatg gcgcagcaac aggttttcga tctcttcggc
     9481 agcgatcttc tcgccgccac ggttgatctg atctttctcg cgcccctgca cggtgatgta
     9541 accctctgga tcaatagaga tcagatcgcc ggaacagtaa aaaccgttgg catcaaaggc
     9601 gctggcattg tgctgcgggc ttttgtaata gccacggaag gtatacggcc cgcgcgtcat
     9661 caggcgtccg acttcccctt gcggcagtgg atttccttcg gcatcggcaa cccatacttc
     9721 gtcatccgga cacattgggt aaccctgggt atggataatt ttctccgcgc tatcatcaag
     9781 acgggtatag ttcaccagcc cttccgccat accaaacacc tgctgcaact ggcaaccaat
     9841 ctcagcggga atacgcgccg caagcgtggc agaaagacgt gcgccaccga cctgtaacag
     9901 tttcagcgag gcaagctgcg cccggctttc gccttcggtc agcgcctgca accacaggct
     9961 gactgccggc ggcaccagcg cggtaacgtt aatctgatgt ttttcaatca atgggaagca
    10021 aagcgtggcg ctgggatcgg ccgccagaac aaccgttcct ccggcaagaa agacgcccag
    10081 cgatcctggc gaactcatgg cgtagttatg agccgccggg atcgcgcaca ggtagcgcgt
    10141 ctgttgtgtg aactgacaaa tctcgacgct acgacgcacg ctgtagtagt agtcgttatg
    10201 agtgcgcggg atcagtttcg gtgtgccggt ggtgccgccg gaaagctgga aataggccac
    10261 ttcatcagca ggtgacggcg tggcagtaaa atcctcagcc ggatggttaa tcgcatcctg
    10321 caagttatgc tcaccgctgt cgttgtgcag ttgcaccacg cgaatggagg agtgctctgc
    10381 aataaatgta ttgaggaaat catccccgct aaatagcgca tgttggcgat cggcaatcag
    10441 taacgccggt tcaatctggc tggcataggc gttcagttca ctacgctgat ggctgaacag
    10501 cgccagcacc ggcgcaacgc ccagtttcag cagcgcgaaa aaggtaatgt aaagttcagc
    10561 gacgttaccc agttgtacca gcgcggtttc accgggttta atgccctgac ggcgcaaact
    10621 acaggcgaga ttattcgccg cctgattcag ctcccgataa ctcaactgcc gctcgccgtc
    10681 gataaccgcg atgctgtcac tcgcagcgtg gcgagtcaga atgtcagtca gtggcaaatc
    10741 ctgccagtag cctttttccc gatagcgacg ggcaaactct tccggccagc gggtaaatgg
    10801 aatgctcatc atcgctcctt aatgcaatcc aaaaacgttc aacatggtag aaagtttgac
    10861 gcctgtttcg cgccactcac ccaacggtga cgacgcaggc acaatccccg ctccggcaaa
    10921 cagacgcacc tgattttccc gcagcttcgc gcagcggatg gtcaccaccc attcgccgtt
    10981 accttcgctg tcacaccaac ccacaatgcc gccaaacagt tcgcggtcga acggctccag
    11041 ttcagcaata acctgggtcg cggcctgatg tgggaaaccg ctcagcgcgg gggtcggatg
    11101 cagcagacaa gccagagtca gtgcgttttc ttgcgaattc gctttacctt caaagggagt
    11161 tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga gaggagggaa cgtgtaactc
    11221 actactgcgt tcgcgcagta cctctttcat cgcctgagtc accagttcat gttcatggcg
    11281 atctttttct gacgccagca gacgattacc cgcttcgcga tcgagcactt catccggctg
    11341 acgacgcgcg gaaccggcta acggaatgga gctaaaacgc tcgccgtctt tacgtagcag
    11401 cagttccggg ctggccccca gcagaacgcc accatctgcc aacggcacat ggaagttgta
    11461 actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg ccactatcaa tggcggcgtc
    11521 agtggtgata tcaatcaacc gtgacaacac cactttgtcg acctgcggcg tggcggtaag
    11581 tgcggcggcg cgggcaacca tctgttcaaa cgtggtttgt tccggaattg cctggcgttc
    11641 caccacattc agcaactggc tgcgggtgaa acggcgtgcg gaagcttgtt tttcctgacg
    11701 ggagaacgac tgccaggatt cgggaatata cagcgacgaa ggctgacgtg gatcgaaggg
    11761 aatcgccccg accatcaccg gatttttgat gccctgcgct ttggcatcgg caaacagcgc
    11821 ggcgagtttt tgctggaagg gactgtcagg cgaatccccg ttcacagccg gttcatcgaa
    11881 gcgggcgaaa catcctgacg tcgtaaaact gcggtacggc gacataaaga aaaagcgatt
    11941 gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc agtgacgtat ccatatcatc
    12001 ctccacaaaa tgataaaggc tttaataatg attatcattt atattttcgg tcgctaacct
    12061 aaaagcaaac gcgcactatg tcaactcttg aggtaacgca atctgcaact cggggttgca
    12121 tctggttgtg gttacaatga aaatgagaag cattaacttc attaataaca ggaagctgat
    12181 ttgtgagact cgccccgctc taccgcaatg cccttctatt aacaggactt ttgctttcag
    12241 gaatagccgc agttcaggcc gccgactggc cgcgtcagat tactgacagc cgtggcattc
    12301 ataccctgga aagccagccg cagcgtattg tttccaccag cgtcaccctg accggctcac
    12361 tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac cacgccaaat aaccgcgtcg
    12421 cggatgacca gggcttttta cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac
    12481 ggctctatat cggcgaaccg agtgcagaag ccgttgccgc gcaaatgccg gatctgattt
    12541 taattagcgc aaccggcggt gattcggcgc tggcgctgta cgatcagctt tccaccatcg
    12601 cgccgacatt aatcatcaat tacgacgaca aaagctggca gtcgctgtta acgcagcttg
    12661 gcgaaattac cgggcatgag aaacaagcgg cagagcggat tgcacagttt gataagcaac
    12721 tggcggcggc gaaagagcaa atcaaattac cgccgcagcc ggtcactgcc attgtctata
    12781 ccgccgctgc acacagtgcc aatctctgga cgccggaatc agcacaaggg cagatgctgg
    12841 aacaactcgg ctttacgctg gcgaagttgc ccgcaggctt aaacgccagc caaagccagg
    12901 gcaaacgcca tgacatcatt cagcttggtg gggaaaatct ggctgcaggg ttaaatggcg
    12961 agtcactatt cctgttcgcc ggtgatcaga aagatgccga tgctatttat gctaatccgc
    13021 tgctcgcgca cctgcctgca gtacaaaaca agcaggttta tgcgctggga accgagacgt
    13081 tccgtctgga ttactacagc gccatgcaag tgctggaacg acttaaggcg ctgttttaag
    13141 cattaaccgt cagacgctgt cacctgcggc ggcgtctggc gaaaacgtcg caactccacc
    13201 agcaccagca ataacaacac gccgataatc aacaaaccaa aaccgcttgc gcttgcggaa
    13261 gcaaccggtg tcatcatcgc tcccaaaccg cccagcagcg ccgcgcctat ggcatcgccc
    13321 gtcacgtttt gtgccgtcca caaaccgtta atccgcccta acatcgcttc cggggtttgc
    13381 gtttgcagca ttgtgtattg cagcaacgag ctgaccgcac tcaaccagcc gaacagcgcc
    13441 agacaaacca cgcctaaaat ccacatcggc atcaggccaa acagaccaat ggcgaggaac
    13501 gatcccagcg tggagagcag catcaataac cctggtcgcg cgctatgcgc cagcttcccg
    13561 ctggttaacg cgccaatagc cgcgccgagc gggatcgccg catagagaaa accaatctgt
    13621 gccgccgaca tctgccagtt gtcagccagc gccggataca gtacccgcac cgcactcgcc
    13681 atcgtcaata aaccacccag cagcgcaatc cctcctacca gcgggctggc gagcagaaaa
    13741 cgaaatcctg ccagtaatga tttcaacgga tgctcacgcg gctgcggtgg cggtggcaac
    13801 gccggaaggc ttaacaacgg cagcaaggta ataaacgtgc ccgccgccgc cagtccgtag
    13861 ttccaggcca caccgccagt cgccagcagt aaaccgccaa tcatgggcga aatcaccgac
    13921 cccagacgca cggtcaacat ggtgatcgcc ccggcctgca ttaagttttc acgccctacc
    13981 agtgcaggtg tcgccgccag tagcgccgta acgccaagtg atgcgaaaaa accatcccat
    14041 aaaccaagta aatagattgc cagcaatgac ggttcaggca gcagtgcatt aaggcacagt
    14101 ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa tcactttttt gcgttcatag
    14161 cgatccgcca gcacaccgcc gaccatcagg ccaacaaaca tcgcgccgcc ggtcagcgtc
    14221 accgaaagcc ccacctgcca ggtggaatgt gtcatcatct ggatctgcac cggcaccgcg
    14281 acgccgagca aacccagaga aacaatagag atgaaacgag cgaggaatac tgcgcgaaac
    14341 gccgggtgcg ttttcaacag gctgaggtta agcagccagg attgtttatt cattacaatg
    14401 ccttgccatc aaatatttta aatacctatt cttaggctgc acatgctaac atatccaaat
    14461 aagatcgata acgataatta atttcattat catggaagtt cgtatgtctg gttctgttgc
    14521 cgtgacacgc gccattgccg tgcccggatt gctgttatta ctgattatcg cgacggcatt
    14581 aagtctgctc attggggcaa aatcactccc cgcttccgta gtgctggagg ccttctccgg
    14641 cacctgccag agcgccgact gcaccatcgt gctcgacgcg cggctgccgc gtacccttgc
    14701 cggtttactg gcaggcggcg cgcttggcct tgccggggcg ttaatgcaaa ccctcacccg
    14761 aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc ggagccagct ttgccattgt
    14821 gctgggtgcg gcgctgtttg gttactcttc cgcacaggaa caactggcga tggccttcgc
    14881 cggggcgctg gtggcctcgt tgattgttgc ctttaccggc agccagggcg gcgggcagtt
    14941 aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg gcagtgctgg aagggctgac
    15001 cagcggcatc gccctgctta atcctgacgt ctacgatcag ttgcgtttct ggcaagccgg
    15061 ttcgctggat attcgcaatc tacatacctt aaaagtggtg ctgatcccgg tgctgatcgc
    15121 cggagcaacc gcgctattac tgagccgcgc gctgaacagt ttgagcctcg gcagcgacac
    15181 cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg attggtctgc tggcgattac
    15241 cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt gcctttatcg gcctgatgat
    15301 gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc tggtcgctgc ccgtcacgct
    15361 acttgctacc cctgccctgc tgctgtttgc cgatatcatc gggcgggtga ttgtgcccgg
    15421 cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca ccggtgctga tcttcctcgt
    15481 gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc gccgattact catcacctgt
    15541 ttgctgctgg tttccgcctg tgtggttgca ggtatctggg gattacgcag cggtgccgtc
    15601 acgctggaaa cctcgcaggt attcgccgcg ctgatgggcg atgcgccgcg cagtatgacg
    15661 atggtggtca ccgaatggcg tttaccacgc gtgctgatgg cgctgttgat tggcgcagca
    15721 ctgggcgtca gtggcgcgat ttttcagtcg ctgatgcgta acccgctcgg cagccctgac
    15781 gtaatgggtt ttaacaccgg ggcgtggagc ggcgtgctgg tggcgatggt gctgtttggt
    15841 caggacctga cggctatcgc gctggcagca atggtgggcg gcattatcac ttcgctgttg
    15901 gtctggctgc tcgcctggcg taacggtatc gacacctttc ggttgattat tatcggtatc
    15961 ggcgttcgcg ccatgctggt ggcctttaat acctggctgt tgctgaaagc gtctttagaa
    16021 acggcgctaa cagcaggttt gtggaatgcc ggatcgctca acggcctgac gtgggcaaaa
    16081 acctcgcctt ccgcacccat cattatattg atgctaattg ccgccgcctt actggtacgc
    16141 cggatgcgct tgctggaaat gggcgatgat accgcgtgtg cgctgggcgt cagcgtcgaa
    16201 cgttcgcgtc tgttaatgat gctggttgca gtggtgctta ccgctgcggc aacagcgctt
    16261 gccgggccga tttcctttat tgctttagtc gcgccgcaca ttgcccgacg cattagcggc
    16321 accgctcgct gggggctaac ccaggcggcg ctgtgcgggg cgttgttact gctggcggcc
    16381 gatctctgcg cccaacaact gtttatgccg tatcaacttc cggttggcgt cgttaccgtc
    16441 agcctcggcg gtatttacct tatcgtcttg ttaattcagg agtctcgcaa aaaatgaccg
    16501 aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg atatggcaaa tataccgttg
    16561 cggaaaatct gactgtagaa atacctgatg gtcacttcac ggcaattatc gggccaaatg
    16621 gctgcggtaa atccacgttg ctgcgtacct taagccgcct gatgacgcct gctcacgggc
    16681 atgtctggct ggatggcgag cacattcaac attacgccag taaagaagtt gcacgccgga
    16741 ttggtctgtt ggcgcaaaat gctaccacgc cgggcgacat caccgtgcag gagctggtgg
    16801 cgcgtgggcg ttatccgcat caaccgctgt ttacccgttg gcgcaaagaa gatgaagagg
    16861 cggtaacgaa agcgatgcag gccacgggaa taactcatct tgcagatcaa agcgtggata
    16921 ccctttccgg tgggcaacgc cagcgagcgt ggatcgcgat ggtgctggcc caggaaacgg
    16981 cgattatgct gctcgacgaa cccacgacct ggctggatat cagtcatcag attgatctac
    17041 tggaactgtt aagcgaactg aaccgcgaga aaggctatac cctggcggcg gtgctgcacg
    17101 atcttaatca ggcctgtcgt tacgccagcc atttgattgc attgcgggaa gggaaaattg
    17161 ttgctcaggg agcgccgaag gagattgtca ctgctgaatt gatcgagcgc atttatggtc
    17221 tccgctgcat gattattgac gatccggtag ccggaacgcc gcttgtggtg ccgctcggac
    17281 gaacggcacc ttcaaccgca aagatttaaa ctaagtggtc tgccatcatg gcatcctgct
    17341 ttctggatgc catcgcatag cgcaataaca cgctaccaca agccaccatc ccgccgatca
    17401 gcgcggaaag gatcacaata atcgccttac ccggaccgtc ttttttcact ggcaatgacg
    17461 ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt gatatttgct tttgttaact
    17521 gctcaacaag atactgacga ttacgtaatt caccgttcag ttccgcaacg tcagtgaccg
    17581 ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc gagagaaata gagaaatcgg
    17641 ggtcatcttt aacggcctga ccattactgt aaacgggctt tttaattcct gccgcgttgg
    17701 caatgtcgag tgaataattg aggcgctgaa tgtttgcatc aagttgattt ttcattttaa
    17761 tgcggtcctg agccagtttt tctttttcaa actgggtttt gatctccagt ttattacgga
    17821 cgttttctat cgactctttc accaccaacg cagagatata atcgatatac ccgctcaaaa
    17881 cggtctgcgc ctcttcactg gttggcgcgg taaaacttag cgtccaggag gtatacagtg
    17941 acggttcatc ttttttctta ctggcattgt catcaaccgc tttcattttt tcgctcaatg
    18001 cgacaattgc gcgatgcaaa tccagttcgt cgattttcgc ctcttttaat tggtccatca
    18061 cataaggtga tgaacgcagg tactcttcca gcaagctaac cgactgaaac ttcttgataa
    18121 acaggttaaa tgcttctgta cgatcaattt tgatatccag atccagcaca cgaagcttag
    18181 tgaatgtttt ctccaactct tgccactgaa caggttctgg aggcgtgaca accgccgcgc
    18241 tggtccattt ttgcggcagg atgaaagaga tcagcaagcc tgcgcaggca aacgcaaaaa
    18301 cgaccgccat gaccgttttt ttggcccgcc ataaaactga gattagatta agtaaatcaa
    18361 tttcattatt actgggcgac gccagaggat aatcgggaaa atgagcgtca cttccctgtt
    18421 taatattcag tgatgacatg gggtataacc tgaaaatgag tccaataggg ggaaatctcc
    18481 acggcgctca ctttaccagt tggtgagaat tatccgaagc tgaagtttgt aaattcattc
    18541 cactgaatgc aacattcact ctatttaaga aaaataacca gcaaacttat aaattatata
    18601 aatgataaac ccataattat ttcctgcaat tatgggttta tagataatgt taatttatct
    18661 gtttagcgtt gcgcgaataa tcggcccaat tttttcaaat gcccctggag agataatatc
    18721 cacatgcgca caatcctgac gatagatatc cagctccgca atccacggcg accaggcgcg
    18781 ttcaggactc ataccttcct gaagcgtgcg ttcagcaaca aacaacgtcg ctttaccgtc
    18841 aaacggtacg ctatgagcag tcgtcagcag gcgcacagca tcagcgtagt tgccttcaat
    18901 ggtggtaaac aactccgttg aagtacttcc ctgctgtgcc gccaggaagg cctcgcgctc
    18961 gcggttaatc tccgccagca cttccgggtc cagaccatta gcttcttttt cctgccagtt
    19021 ttgcgtttct ggcggccagg tatccagcaa gccaagaaat gccacctgtt cgccacgggc
    19081 acgcagccgc gccgcaatac cttgcgccag cgtaccgcca agtgaataac ccagcaggta
    19141 ataagggccg tgcggttgtt gttcaagtaa cgttgccaga tgcgcttcgc agacttcatc
    19201 caggttcgtc gctgtctgca tggggccatg agggcgcggc gactgaatgc cgataatcga
    19261 ccatagtgga tcgagataac gcgagagcac gctaaactgc caggcaaaac cggatgccgg
    19321 atggaaacaa aacagcgtcg ggccattacc ttcacgcaac ggcagaatgg tttcgaatcc
    19381 catgcgccgg gagctgtcct cttcaccatc aataatcgtt gccagtttgg cgacggttga
    19441 cgcgaccatc acctgccccg gcgtcacctg acgggcaaac tgccgactta actgcgctgc
    19501 cagtttcatt gccagtagcg aatgaccacc aagcgcgaag aaatcagcat cgacatcctg
    19561 cacgtcacaa cccagtaacg acgagaatgc cgcggcgata atcgtttcac tgcccgcttt
    19621 cggcgcacgc cccggcgttt gtgccttaag ttcaggcaac ggtaaggctt tgcgatccag
    19681 cttgccgttg gcgctaagag gtaactgtgg aagttgcagc agaacgaccg gcaccatatg
    19741 cggcggcaat gtttcgcgaa gctgtgcctg taatgcgctg gtatccaacg gcagacctga
    19801 ttgcgacacc agatagccca ccaactgacg cgcatcacca ccggtggctg ccgcctggtt
    19861 aatcacacag gcgtgggtaa cggcttgttc gacatccggc agcgcctgca tcacgcgatc
    19921 gatttcgccc agttcgatac gctgcccgcg aatttttagc tgatcgtcac tgcgcccgag
    19981 gtactccacc gcgccgttat ccagccagcg ggcaacgtct ccggtacggt acatccgttc
    20041 gccaggagca aaaggatcgg caataaaacg gctggcggtc agatcgggtc gtccaagata
    20101 cccctgcgcc agttgaatgc cggtgagata gagatcaccc gccacacccg gcggcaccgg
    20161 atgcatcatc gcatcaagaa tacgcagacc cgtattccat accggataac caatcggcac
    20221 actgctgccg cgcacctgtg ccagttcctc gccaaaagcc ggataccagc tgacatctac
    20281 cgccgcttcc gtcgggccat atagattatg caacggcgcg cccgttaact gttgccattc
    20341 gcggcataaa tcagccggta aggcctcacc actacagaaa acctgtttca acgtcgcgca
    20401 actctggcga gcggtttgcg gagtcagcga tgcaacaaat gccgccagca tcgacggcac
    20461 aaagtgcgtg gtcgttacgc catattcggc aaagaattgc tgcatagcga gcgggtcgcg
    20521 gtgcgcttcc ggttcagcca tcaccagttt tgccccggca ataaacggcc agaaaaactc
    20581 ccacaccgag acatcaaaac tgcacggcgt tttttgggca acgacatctt cgcctgtaag
    20641 cgggtagtga ttttgcatcc ataacaggcg gttaacgata gccgtctgcc cgaccattac
    20701 ccctttcggt ctgccggtgg aaccagaggt aaagatgata taagccgtgt ggtgcggttg
    20761 tgaaagttgc agcggcgcac tgccctgcgg tgtaagcggg gcgttatagc aaaggttggt
    20821 taaatctgga acatcggcaa agcgcggcag ttgatcatcg gtggtgatta acagcgacgg
    20881 acgcgcatct tccagcatca ttttcaggcg atcgtccgga taccccgtat ccagcggtaa
    20941 ccaggccgca cctgcttcaa caatcgcatg tagcgccagg gtcaaaaaga ccgagcgcgg
    21001 taatgccacc gccacactgt cccctggttt aacgccgtgc tcacgcagca gattcgccag
    21061 cgccaccacc tgctcgcgca tttcccgata gctgaactgg taacgcgcat ctgccagcgc
    21121 cggagcatcc ggtgtttttg ccgcttgttc tgccaccagc gcgctaagcg tggtttctgg
    21181 aatctcaacc tgagtggcgt tgatctgcgc caactgcgca tactcacctg gcagcataat
    21241 atcgacatcg ccgcacaaca gcgccggatc cgcggcgaac tgggcaatca gcattttcag
    21301 gcgttcagaa tgctggatta acgttggctc atcgtaacgc tgtttattgg caaggatctc
    21361 aatactcaaa tcaccgtgtt catccgggaa cagggccagt tcaaggtcat taaccggccc
    21421 ggttgccagg gtatgggttt gcgcctgaac accaggaatg tccagttggt aatcaaatac
    21481 tttgatattg agtaccggac cgaacagcgg ttcatcacct gccgctcgcc cactgtcacg
    21541 gacaatttgt tcggcatcgt aacgttgatg gcgacgcatt tttttcagtt gtgctgccag
    21601 tcgggttgcc agttccggca gcgtttcttg cgccgcaatg tgaataccca acggtaaaac
    21661 gttgagcacg ggtccggtag ccgtcagcgc cgccgagccc agtcgacgca taaagataaa
    21721 tccggcggcg tagtccatgc gattgcacaa tcgccccagc cacaaggctg ccagcgcaag
    21781 ggctaaatcg gtacgctgca cacctgaaag ttgcgcagcc aactggcgga attccccgtc
    21841 ggtaaattcc agtttcaggc gcagaatatc tgccgaggcg ctacgccccg gtaaaggtgc
    21901 cggagaaagt gacgcgggcg gtggcagttg gcgacgctgt tccacccaga atgccgcatc
    21961 acgctgccag gcttcgcttt cgcggtattg ctggtactct tccactacat cagcgaaagg
    22021 cgtaaacggc gaagcaggcg ttggttcgcc acgcagcaat gcgcagtaaa tgttggcgat
    22081 ctggcgggta atggccggaa aactgaagcc atcgaccagt aaatggtgat aacgctgata
    22141 ccagtaccag cggttatctg ccacctgtat cagctggtga aagaccagtg gtttaccgct
    22201 atcgacgcgc aaatcttgtt gcaaatccgc ctgcattaat gcctgcgcag taccgtgcgg
    22261 atcaatgttg gttcgcaggt cgataatttc tggcagttcg aatatcagcg catcatcgac
    22321 ccattgccag acttcgccgt tatcttccgt aaaacgcatc cgcaacgtat ctgcttgcgc
    22381 taatccggca accaccgcgc gggccagtaa tggcgcatca acctctccgg ttaactcaac
    22441 gtaatgcgcc acgctccagg cggagggtaa atccgacagt ttttctgcca tccagatgcc
    22501 gggctgtgcg gcgaccaaag gtaaatgctg gctcattgtg cctcctgcaa ctgggtgaaa
    22561 ttcgccggtg tcagagtacg ccagtgggct tcaagccact gctggcagga ctcttgtgac
    22621 tgcggctgac acacaatgtc ccagcctgcc ggtaaggcgc attgttgcgg ccacagactg
    22681 aattgcccct gcgcattgcg caaaatgtaa aacactccct gtggatcatc gaagggatta
    22741 ctgaatgcca tattcaactc ctgtcatgga aaagtggttg ccagaggtcg attaacccct
    22801 gcatcaagcc accgcgccaa caaagcgcat catgtccgcc gtcaacctga cgccagaaaa
    22861 tggattcttt tatagggtgt aattgcgcat acagcgcctg attggcccgc atgatcatcg
    22921 gctcgcgaat acccgcttcc agcacaatgc gcagaccttc ggcgctaact tcaccagctt
    22981 ctagcttttc aagtaacacg ccctcttgct gcccgccccg atgcggccac cagtacgatc
    23041 ctgactggct taatacacag ccaaagcgtt caggccagtg cagtccggca tacagcgcgg
    23101 acagcccacc aaaactctgc ccggcgacca cggtgcgatc ggcgcgatcg ctaaaagggg
    23161 caatagcttt caccaggggc aataactctt gctgtactgc cagccagaaa tccgcattac
    23221 acggcagttc gtgggcgcgg tgcgtggtgt cgatagcgtc gatcaacaca tacacggcgg
    23281 gaggaagttg ctgacgatgg gtcagcgaag tcagcacagg ccagacgggc atactttgcg
    23341 cccaaaattc gccatcgagc aaaactgcca gcgggcgttc ttctgctgtt acatcgccgg
    23401 tggtaaaaat ccatacacga cgtgaatttt tcaaccgttc acttttccag ataatttctt
    23461 tggctggtat ttctggcgct tgcggacaat cccatcccgg ttgcagaggc gcttgcggca
    23521 tttcgagtgc agaaacagcg tgccctcgcc cgcctttcca gctttgtgga ttcagcggat
    23581 cggctatcgc ctggggtaat agttttcgcc agccttcacg taattcgagg cgatcggggg
    23641 atggtgcaga aaaaatgtca tcgcgttcgg tgggaataaa gcagtagctg ccgcgccagt
    23701 tggcattgag ttgtgtcgtc cactgccaga catcagtacc tgcaattcgc tgcatcgact
    23761 ggggctggct gttctgatga tgatcggtca caccagtgat gtagacccat acacgcttta
    23821 tcgtcgagta ttcttcagaa ccttggggat cacgccacca gaaagtgacc tcaaacattt
    23881 cgtcattcag acgctgccat tccgggccat gtttcgactg ccaccagctc tcacttccta
    23941 cttttaacgc cgtcacacca taaccccatg tttactgtgc aatttttcat tgattgcaga
    24001 aatatattga taatattatt gataactatt tgcatttgca atagcgtaat ggcgcgccgt
    24061 gggaagcgcg gacattaatt aaccaactgc actgcgtgtc tttcaggatc aaaggttttc
    24121 gcggtagcgg gatgcgtcgt gttgatgacg accatgcccg acagttgcaa ttcgtggcaa
    24181 aaatgcagga ataaaacaat gaacaagaag attcattccc tggccttgtt ggtcaatctg
    24241 gggatttatg gggtagcgca ggcacaagag ccgaccgata ctcctgtttc acatgacgat
    24301 accattgtcg ttaccgccgc cgagcagaac ttacaggcgc ctggcgtttc gaccatcacc
    24361 gcagatgaaa tccgcaaaaa ccccgttgcc cgcgatgtgt cggagatcat ccgtaccatg
    24421 ccaggcgtta acctgaccgg taactccacc agtggtcagc gagggaataa ccgacagatt
    24481 gatattcgcg gtatgggtcc ggaaaacacg ctgattttga ttgacggcaa gccggtaagc
    24541 agccgtaact cggtgcgtca gggctggcgt ggcgagcgcg atacccgtgg tgatacctcc
    24601 tgggtgccac ctgaaatgat tgaacgtatt gaagttctgc gtggtccggc agctgcgcgt
    24661 tatggcaacg gcgcggcggg cggcgtggtt aacatcatta ccaaaaaagg cagcggcgag
    24721 tggcacggct cctgggacgc atatttcaat gcgccagaac ataaagagga aggtgccacc
    24781 aaacgcacta actttagcct gaccggtccg ctgggcgacg aattcagctt ccgcttgtat
    24841 ggcaacctcg acaaaactca ggctgacgcg tgggatatca accagggcca tcagtccgcg
    24901 cgtgccggaa cgtatgccac gacgttacca gccgggcgcg aaggggtgat caataaagat
    24961 attaacggcg tggtgcgctg ggacttcgca ccattgcaat cgctggaact ggaagcgggt
    25021 tacagccgcc agggtaacct gtatgcgggc gacacccaga acaccaactc cgattcctat
    25081 acccgctcga aatatggcga tgaaaccaac cgtctgtatc gccagaacta ctcgctgacc
    25141 tggaacggcg gctgggataa cggcgtgacc accagcaact gggtgcagta cgaacacacc
    25201 cgtaactcgc gtattccgga aggtctggcg ggcggtaccg aagggaaatt taacgaaaaa
    25261 gcgacacagg atttcgtcga tatcgatctt gatgacgtga tgctgcacag cgaagttaac
    25321 ctgccgattg atttcctcgt taaccaaacg ctgacgctgg gtacggagtg gaatcagcaa
    25381 cggatgaagg acttaagttc caacacccag gcactgaccg gaacgaatac cggtggcgct
    25441 attgatggcg tgagtgccac cgaccgtagc ccgtattcaa aagcagaaat tttctcgctg
    25501 tttgccgaaa acaacatgga gctgactgac agcaccatcg taacgccggg gctgcgtttc
    25561 gatcatcaca gtattgtcgg caataactgg agcccggcgc tgaacatatc gcaaggttta
    25621 ggcgatgact tcacgctcaa aatgggcatt gcccgtgctt ataaagcgcc gagcctgtac
    25681 cagactaacc cgaactacat tctctacagt aaaggtcagg gctgctatgc cagcgcgggc
    25741 ggctgctatc tgcaaggtaa tgatgacctg aaagcggaaa ccagtatcaa caaggagatt
    25801 ggtctggagt tcaaacgtga cggctggctg gcgggcgtga cctggttccg taacgattat
    25861 cgcaataaga ttgaagcggg ctatgtggct gtagggcaaa acgcagccgg caccgatctc
    25921 tatcagtggg ataacgtgcc gaaagcggtg gttgaaggtc tggaaggatc gttaaacgta
    25981 ccggttagcg aaacggtgat gtggactaat aacatcactt atatgctgaa gagtgaaaac
    26041 aaaaccacgg gcgaccgttt gtcgatcatc ccggagtata cgttgaactc aacgctgagc
    26101 tggcaggcac gagaagattt gtcgatgcaa acgaccttca cctggtacgg taagcagcag
    26161 ccgaagaagt acaactataa aggtcagcca gcggttggac cggaaaccaa agaaattagt
    26221 ccttacagca ttgttgggct gagcgcgacg tgggatgtga cgaagaatgt cagtctgacc
    26281 ggcggcgtgg acaatctgtt cgacaaacgt ttgtggcgtg cgggtaatgc ccagaccacg
    26341 ggcgatttgg caggggccaa ctatatcgcc ggtgccgggg cgtataccta taacgagccg
    26401 ggacgtatac ctataacgag ccgggacgta cgtggtatat gagcgtaaac actcacttct
    26461 gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca tgccggatgc ggcgtgaacg
    26521 ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga
    26581 taagcgtagc gcaccaggca gttttgcgtt tgtcatcagt ctcgaatatg gtcgatatga
    26641 aaactacgca tacctccctc ccctttgccg aacatacgct gcattttgtt gagttcgatc
    26701 cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa ctgcaacacg
    26761 ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt tatgccttgc
    26821 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg
    26881 cggaggtata cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc
    26941 aaccgattgg cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag
    27001 acaacattat tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc
    27061 tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa
    27121 ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc
    27181 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc
    27241 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgaccg
    27301 cgggcgaaag tgtaaagcag gtgccttacc atcctgacct gacaaccgga tatgcgggga
    27361 ttactccccg cacaacggct acttcttcgg ttcgtaagcg agaacagccc tgaactcaat
    27421 attacgttcc ttcaccgtaa actcacacag cgagtcgccg accagaagcg taaatcccag
    27481 caccgtcaaa cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt
    27541 gcctccatgc gaagaaggga ctaccatcca aattgttcag gtttggagtg gcagccccag
    27601 ggttgataga aatatctcct ggggcttcat ctttccagac ctcagagtta acctgaaacc
    27661 ggaaagcttc aggcacccgc cgttatctta cccttcctca acaaaatggc tgtttattta
    27721 tacagtaaat cctgtttcgg acttctcact cctcatttaa tcaactgaag ctgagcccgt
    27781 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagccgtg ccataacttg
    27841 tcacattgca aaagtagcca aattgcttca ggcaccatca gttcaccatc accaaaaaaa
    27901 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
    27961 aacgccaaca taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
    28021 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
    28081 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
    28141 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
    28201 cgggcaattt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
    28261 aatttgcggc ggtggcacgc acccgtttca gaaatggcag cgtcaggagg tatgcgacaa
    28321 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
    28381 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctacacgg
    28441 cttgtcacgg tttgtgccgc actttatcgc cctttccgcc gcgtcgccgt atatgcaggg
    28501 aacggatacg cgttttgcct cctcacgacc aaatattttt tccgcctttc ccgataatgg
    28561 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gcctgagtta
    28621 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
    28681 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
    28741 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt ttaaacatca
    28801 ggaaaaagat tacctgctgt ataaattcaa ccgtttccag gcctgccgct atgggctgga
    28861 aggcgtcatt accgatccgc acaccggcga tcgtcgaccg ctaacggaag acaccttgcg
    28921 attgctggaa aaaatcgccc cttccgcaca taaaattggt gcatcgagcg caattgaagc
    28981 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
    29041 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
    29101 agtcgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
    29161 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
    29221 ttcctctctt gcctgctgct tcccgccccc gcattggggc tgacgctggc acaaaaactg
    29281 gtgaccacgt tccatctgat ggatcttagt cagctttaca ctttattgtt ttgtctgtgg
    29341 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
    29401 tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc
    29461 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc
    29521 ccttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
    29581 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
    29641 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
    29701 gtatcacatg aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc
    29761 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
    29821 cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta
    29881 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt
    29941 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
    30001 aaaacttacc ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag
    30061 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
    30121 tgccaaatcc gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca
    30181 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
    30241 ccaggaagat gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
    30301 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
    30361 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga
    30421 cgcggtaccc atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga
    30481 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
    30541 cgctacgctg ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc
    30601 ctgacagccg ggaccccatc ccggctcaga ttgctgacaa agtgcgcttt gtttatgccg
    30661 gatgcggcgt gaccgcctta tcctgcctac aagttcgtgc caattcattc aggtaggcat
    30721 gataagcgta gcgcatcagg caattttgta tttgccatca gccctcgcag gaaatccccc
    30781 ctcagattct cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat
    30841 aaaggagacg ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc
    30901 gatcacacca cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg
    30961 catattattt tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca
    31021 cggctttggt tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg
    31081 caggggatta tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca
    31141 gatattctga ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc
    31201 tcgctgctgg atgttatttt gaatctggcg cagaaattcc cggcagcatc tcagttaccg
    31261 ctgaaaggga tatttcaagg gattaaactg atcggcgcga ttctggtcgg cattttgatg
    31321 atctcgctgc tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct
    31381 gccgtgctga tgttggtatt taaagatccg attcttggtc tggtggcagg tattcagctt
    31441 tccgcgaacg atatgctgaa actgggcgac tggctggaga tgccgaaata cggcgcggat
    31501 ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt
    31561 accactattc ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca
    31621 gcatccggcg ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt
    31681 cttgatgaag acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc
    31741 agccgccatc aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcggtatta
    31801 aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt
    31861 aaccatccgc ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat
    31921 aacggtttac cgcttgagat ctatgcgttt accaacaccg tggtgtggct ggaatatgaa
    31981 agcattcagg ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga
    32041 cttcatcagt cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa
    32101 ttaaaaaaac cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt
    32161 gaatgcctta tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg
    32221 gcagcaggat cgctgacaag cgcatatcat ctatcgtgca catcagcagc aaaatcatgc
    32281 cgaggaaggc aatgcagaga tagtttccga acggatagag cagcgcctta aactgtgttt
    32341 cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg cgccagacag atcataatcc
    32401 agttcaacag cagcgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca
    32461 gcagatagtt gattaacacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa
    32521 tcggcacgcc gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgtacagaaa
    32581 ggccaaacag catgcggctg ttagagtaaa ccccgctgtt atacactgac agcgatgcta
    32641 ccagaatgac gaagttcagc gcagaagcta ccacgttgct gtcgagatta tggaaaatca
    32701 tcacaaacgg gctactgttg gatttcactt ccacccacgg atagagcgcc agtaaaacca
    32761 ccagtgaacc gatgtaaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa
    32821 tgcttttttc cggatcgcgc gcttcagcgg cagtaatccc aatcagctcc agaccgccga
    32881 aggagaacat aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac
    32941 caccgtagcg ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca
    33001 gccacaggcc aaagccgatc ataccgatga ttgccagcac tttaatcagc gcaaaccaga
    33061 actcggtttc gccatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga
    33121 aggcggcagc ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg
    33181 cagcggtcag ctctgccatt cccaccagca cgaacatcac ccagtaattc cagccagaga
    33241 ggaagcccgc gaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg
    33301 gctcctcaac caccatttcg ccaagctggc gcataatcag gaaagcgatg atcccggcga
    33361 cgccgtagcc cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa
    33421 acagaccagt accaattgcg ccacccagcg caatcagttg aatatgacgg ttatgtaatc
    33481 cgcgatgaag cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt
    33541 tcacgcattt cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca
    33601 tcgtcgcaag acacaatcca cacggttaaa cggggtatcc tgcttttatt tccgtacccg
    33661 atgtcggtgc agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg
    33721 cgcggtgatc atgccgccaa tcatcggcgc ggcaatccgg ctcatcacct ctgaccctgc
    33781 tcctgttccc cacagaatcg gcaacagacc agcgataatc accgccaccg tcatcgcttt
    33841 cgggcgcacg cgcaggaccg cgccgtgata caacgcctca tccagcttct gctcgctgaa
    33901 tgtttgcgga ttattcaacg acggctcggc ctctatggcg tgacgtaaat acatcagcat
    33961 caccacgcca aattcggcgg cgaccccggc aagggcaata aaaccggtgc ccgtcgccac
    34021 agaaagatga aagcccatcc accacagcaa ccagatgccg cccaccagcg caaacggtac
    34081 gctgctgata atcagcaacg cttcgcccac ccgacggaat gccagataca acagcacgaa
    34141 gatgatcatc agtgtcatcg gcaccatgag tttcagctta tggttggcgc gctccagtag
    34201 ctcgaactgc ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat
    34261 cgctttttgc aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat
    34321 ccagctcgtc gggcgcgcat tctcggtttt cagcattgac ggtccggtag agactttaac
    34381 gtcggccacg tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcaactggcg
    34441 cagtgcctgc ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc
    34501 aatcccttcc accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg
    34561 cacatccgcc accgtcatac cgtaacgcgc ggctttttca cggttaatct caacgttgat
    34621 atagcgccca ccttccagac gctcggcaag cgcagaagct acgcctggca ccgttcgcgc
    34681 cacttcttca atttgctccg ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt
    34741 aatgccgatg gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg
    34801 cacccacaga ttcgccagcc ccggcagacg cacggtgtta tccagttcct caatgatttt
    34861 gtccattgtc atgcctggcc gccactgatc ctgcggctta agctggatgg tcgtttctac
    34921 catctccagc ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg
    34981 cgccacttca ggtacgctca taatcagctt gtcggttttt tgcagcatac tcgccgcctc
    35041 tgctgcggaa atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg
    35101 cggtaaaaat tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc
    35161 tgccaccaac agcgtggttt tcggccagtg cagtactttc agcaatagag gatgataaac
    35221 acgaatcaaa aagcgattga gcgggttact gctttccggc ggaattttgc cacggatcca
    35281 gtaacccatc aggataggga tcactacgat cgccagcagc gccgcacccg ccatcgcata
    35341 cgttttggtg aacgccagcg ggccaaacag acgcccttcc tgcccttcca gggtgaagat
    35401 cgggataaac gacagtgtga taatcagcag actgataaac agcgccggtc ccacttcaac
    35461 agacgcatcg gtaatcacct gccagcgcgt tttattatcc agcgtggtgt caggatgctg
    35521 gtgctgccac tcttccagcc gtttatgcgc attctcgatc atgacgatag cggcatcgac
    35581 catcgccccg acggcaatcg caataccgcc cagcgacata atattggcat tcagcccctg
    35641 gaagtgcatg acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag
    35701 cgccgagcgc acatgccaga gaaacagcgc acagactacc gccaccacaa taaactcttc
    35761 cagcaacttg ccgctgagat tgtcgatggc ccggtcaatg agctggctgc gatcgtatgt
    35821 tgtaactatc tccacgcctt ccggcagact gcttttcagc gtttctagtt tgtccttcac
    35881 ggcggcgatc acttcacggg cgtttttgcc ggagcgtagg atcaccaccc cgcccgcaac
    35941 ttcgccttcg ccgttcagtt cggcaatgcc ccggcgcatc tccgggccaa cctggatctt
    36001 cgcaacatca cgcagataaa cgggcacgcc attttcactg gcttttaaaa cgatgtgatt
    36061 aaagtcgtcg agcgtttgta gatagccgct ggcgcgcacc atatattctg cttccgccag
    36121 ttcgattgac gaaccgcccg cttcctggtt tgaagcatcc agcgcgcttt taacttcggc
    36181 gagactgatg ccatactgcg ccaggcgctg gggatcgata accacctgat actctttcac
    36241 cacgccgccc accgacgcca cttccgcaac gtcagggatg gtttttagct catatttgag
    36301 aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc ttaccgctgc gatccaccag
    36361 tgcatattca tagatccagc caacacccgt ggcatctggc cccagctcgg cgctgactcc
    36421 cgcaggcagc ttaccctgta cctggttgag gtactccagc acccgcgagc gcgcccagta
    36481 cggatcggtg ccatcttcga aaatgacata cacataagag tcgccaaact gcgagaaacc
    36541 gcgcacagtc ttcgcgccag gtaccgacaa catggtggta gttagcggat aagtcacctg
    36601 attttcaacg atttgcggtg cctgcccggg atagctggtt ttaataatca cctgcacatc
    36661 ggagagatcc ggcaacgcat ccactggcgt gttaatgatg gtccaggtgc cccagatact
    36721 cagaaataac gcgcccatca gcaccagaaa acggttcgcc accgagcgac gaataatcca
    36781 ttcaatcatt ggttattccc tcaatgcgca tgggtagcac tttcagagcg catccgctcc
    36841 agtgcgccag aaatattggc ttcggaatca atcaggaaca ggccgctgga aaccaccttt
    36901 tcaccttccg ccagaccaga gcgtaacgcg gtgacacctt gcgatgcctg gaaaacggca
    36961 acgcgtttcg gtacaaagcg cccgtcggca tcaacggtaa tcacccgctg ttcgttgccg
    37021 gtatcaatca gcgcttgtga cggaatgagc agcatcggtt cgctggcggt attgagttgc
    37081 aaccaggcgt tcattcccgg ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagc
    37141 tgcagcgtgc gggtcgcgga atccacgcca ggtagtagcg tccatttgcg gatggtgagt
    37201 gttttatcag gtcgcgccgg aacggtgagc gtaaactgcg aggcatcttt caccagccag
    37261 gcgatggact ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattttcgcg
    37321 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
    37381 tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggcggcga
    37441 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
    37501 gccgtaccgc cggtttcgcg cagcagtaaa tactcactct gcgcttccac ccagtcggga
    37561 atggtcaggt cgagaagcgg tgtgcccttt tgcactttat cgcccacggt aagcggatac
    37621 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
    37681 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc cgcgcgtgac ggtagccgtt
    37741 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
    37801 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
    37861 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcagtcgac
    37921 gttttttctg cgggcggttc agcccggtca aaccaggtaa aacctgccgc agaaataata
    37981 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
    38041 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
    38101 gccactttgt cgccagtttt aatttcactc attttcgtct gcggagtgat ggtaaagcgc
    38161 atagtcatct ccggccagtt cacggcagca atcggatcgt gatggatggt gatttttttg
    38221 ctttccagat caacaccttt taccacgccg gtggcactaa tgacctgtgg ttgtgcttcg
    38281 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
    38341 ctgaacattg cgacttgcag tgcttttttc atttactctc tcctggagtt aaaaattatt
    38401 gctgccaacc gccaccaagt gcggtatata aagaaatttc gttgacctga cgggcataat
    38461 tcaggtcaag taaggtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
    38521 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcaaagta atttgcagcg
    38581 acgccagata acgctgctgg gcgctgattt gatcgttcag gctttgacgt aatgccagcg
    38641 catctgccac ttctttaaag gcgttctgga ttttctgttc ataattcact accgactgct
    38701 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
    38761 tctcaatttt gggtataaaa ttccacatcc cgctgctggc gttaaataat gacgacagat
    38821 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcgcgtg
    38881 cagcaccaat attggcatta gccgccatta acgcatgttc agcttccata atatccgggc
    38941 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc agactgccgc
    39001 cgtttactgt ctgcgcttgc ggcagcttgc cgtagcttcc caataacagt tgcaatgcat
    39061 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc gctgcgggta ctttcaatca
    39121 ccccgcgagc ctgttccagc gccagaacat tactgctacc tgtcaacagt tgtttttcga
    39181 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
    39241 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaga atatgcaccg
    39301 cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc ggcttcgctc atgttcttta
    39361 agcgcccgaa aaaatcgagg tcaaagctgg cgttaaggcc agtagaaaac tcccgcgtcg
    39421 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcacca ttgagctgtg
    39481 ggtagcggtc ggcatcagtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
    39541 ccatgcgcaa atcccggtta ttctccagcg cctcgctaat cagcgtcttc acctgattat
    39601 caacaaaaaa ggtgcgccag cccgcgttct gatagttatc tgctgcgtta accaggccgt
    39661 tctggctgag tgagaactgc tgaggaacgg gcattgccgg acgctgataa tccggtgcca
    39721 gtgaacaacc ggttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
    39781 gctcataatt tctggtgatt ttataccgcc aactttactc gccaggctct gattttccgg
    39841 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
    39901 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
    39961 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
    40021 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg atctgataat
    40081 cctcgatatt atgctgccgg acgtgaacgg ctgggatatc gtgcgcatgt tacgctccgc
    40141 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
    40201 ggggctggag ttgggggcag atgactacct ggtgaaacca ttcgcttttg ctgaactact
    40261 ggcgcgggtg cgcacattac tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
    40321 ggttgccgat ttgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
    40381 cactttgacc agtaaagagt ttactctgct ggagtttttt ctccgccatc agggcgaagt
    40441 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aattttgaca gcgacactaa
    40501 cgccattgat gtggcggtga agcggctgcg cggcaaaatc gacaacgact ttgagccgaa
    40561 gctaattcag accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
    40621 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
    40681 tcgcggcgtt tttcgccttt gcatggatca tgatccactc agtaaaagtg cattttgccg
    40741 agcaggatat taatgattta aaagagatta gcgccaccct tgaacgcgta ctaaatcacc
    40801 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
    40861 acgtgttgat ttccctggca gatagccacg gtaaaacggt gtatcactcc cccggtgcgc
    40921 cggatatccg cgagtttacg cgtgacgcca tacccgataa agacgctcag ggtggcgagg
    40981 tgtatctcct ttccggcccg acgatgatga tgccaggcca cggtcacggg catatggaac
    41041 acagcaactg gcggatgatc agtttgccag ttggcccact ggttgacggc aaaccgattt
    41101 atacgctcta catcgcgctt tcgatcgatt ttcatcttca ttacataaat gatttgatga
    41161 ataaacttgt aatgaccgca tcgatcatca gcatcctgat tgtctttatc gtgctattgg
    41221 cggtacataa aggtcacgcg ccgattcgca gcgtcagccg tcaaatccag aatattacct
    41281 cgaaagatct cgacgttcgc ctcgacccgc agaccgtgcc gattgagctg gaacagctgg
    41341 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttacccgc cagtccaatt
    41401 tctcagcgga tatcgctcac gaaattcgta cgccgattac caatctcatc acgcaaacgg
    41461 aaatcgccct cagccagtct cgcagccaga aggagctgga agatgtgctc tactctaatc
    41521 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gcccaggccg
    41581 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa atcggcaaag
    41641 tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt ggagttgcgg tttgttggcg
    41701 acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg ggcgttaagc aacctgcttt
    41761 ctaatgccct gcgttatacg ccacccagtg aggctattgt agtgcgctgc cagacggtcg
    41821 atcaccaggt gcaagttatc gtcgaaaacc ccggtactcc cattgcgccc gagcacttac
    41881 cgcgattgtt tgaccgtttc taccgcgttg acccttcccg ccagcgaaaa ggcgaaggta
    41941 gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc acataaaggc acggttgcag
    42001 taacgtcaga tgcgcggggg acaaggtttg tgattgtgtt gccggagagg gggtgattat
    42061 taagttcaaa ttcagggcga gggtttacag cgtttttcgc taaatttcca tcattataaa
    42121 caagccacct atcatataaa gcaatgaata attaaatcgc gattaatcat cattaatttt
    42181 tatattattc acccaatcca tttataaagt gaatttgata aattcctaat ttaaaatatc
    42241 cactattgag ccagtattta acaacgttaa gatgtgcaat gtgcagatca tgttatcagg
    42301 gtggagcggt agcatatatt ttttaagcaa tcattcattt taatgatgtt aattgggtta
    42361 tttagttgtg aataaaaaca tattatgaat attggtttta ctttgcgttt ttgttactcg
    42421 ctgtgatgtg cgcacctgtc ctgaataacg tcaggaaaca ataagttgag cttgtgatta
    42481 tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa attctcacat
    42541 attcatagca ttttttgttc cttccctaat aacgtcttaa gtcgtcccgc ttatctcatt
    42601 atccgcagtt gtacggtgcg gattattatt cattttaccg gaggttgcta tgtcaaccgg
    42661 attacgtttc acactggaag tggacggcct gccaccggac gcttttgcgg tggtttcctt
    42721 tcatctcacc cagtcactct cttcgctctt ttccctcgat ctctccctgg tcagccagca
    42781 gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga caatatggca
    42841 gggtgatgat gtacagcgcc gggtgaaagg tgtggtgacc tggtttgaac t
//
LOCUS       DAZGCQ010000035        42698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000035 DAZGCQ010000000
VERSION     DAZGCQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.035"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..350
                     /locus_tag="ACHK07_002015"
     CDS             <1..350
                     /locus_tag="ACHK07_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8102346.1"
                     /translation="DEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAG
                     KLPEAFFWTDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSA
                     DEILAYRVGWAQE"
     gene            complement(790..1119)
                     /locus_tag="ACHK07_002016"
     CDS             complement(790..1119)
                     /locus_tag="ACHK07_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8102347.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(1260..1347)
                     /locus_tag="ACHK07_002017"
     tRNA            complement(1260..1347)
                     /locus_tag="ACHK07_002017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1311..1313),aa:Ser,seq:tga)
     gene            1773..2891
                     /gene="hyaA"
                     /locus_tag="ACHK07_002018"
     CDS             1773..2891
                     /gene="hyaA"
                     /locus_tag="ACHK07_002018"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO8102348.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2888..4681
                     /gene="hyaB"
                     /locus_tag="ACHK07_002019"
     CDS             2888..4681
                     /gene="hyaB"
                     /locus_tag="ACHK07_002019"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO8102349.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4700..5407
                     /gene="hyaC"
                     /locus_tag="ACHK07_002020"
     CDS             4700..5407
                     /gene="hyaC"
                     /locus_tag="ACHK07_002020"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO8102350.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5404..5991
                     /gene="hyaD"
                     /locus_tag="ACHK07_002021"
     CDS             5404..5991
                     /gene="hyaD"
                     /locus_tag="ACHK07_002021"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO8102351.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLGAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            5988..6386
                     /gene="hyaE"
                     /locus_tag="ACHK07_002022"
     CDS             5988..6386
                     /gene="hyaE"
                     /locus_tag="ACHK07_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO8102352.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6383..7240
                     /gene="hyaF"
                     /locus_tag="ACHK07_002023"
     CDS             6383..7240
                     /gene="hyaF"
                     /locus_tag="ACHK07_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO8102353.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7374..8918
                     /gene="appC"
                     /locus_tag="ACHK07_002024"
     CDS             7374..8918
                     /gene="appC"
                     /locus_tag="ACHK07_002024"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO8102354.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQSFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8930..10066
                     /gene="appB"
                     /locus_tag="ACHK07_002025"
     CDS             8930..10066
                     /gene="appB"
                     /locus_tag="ACHK07_002025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8102355.1"
                     /translation="MFDYETLRFIWWLLIGVILVAFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10079..10171
                     /gene="cbdX"
                     /locus_tag="ACHK07_002026"
     CDS             10079..10171
                     /gene="cbdX"
                     /locus_tag="ACHK07_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO8102356.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10251..11555
                     /gene="appA"
                     /locus_tag="ACHK07_002027"
     CDS             10251..11555
                     /gene="appA"
                     /locus_tag="ACHK07_002027"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO8102357.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCP
                     QSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11675..13855)
                     /gene="etk"
                     /locus_tag="ACHK07_002028"
     CDS             complement(11675..13855)
                     /gene="etk"
                     /locus_tag="ACHK07_002028"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO8102358.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13875..14321)
                     /gene="etp"
                     /locus_tag="ACHK07_002029"
                     /gene_synonym="yccY"
     CDS             complement(13875..14321)
                     /gene="etp"
                     /locus_tag="ACHK07_002029"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO8102359.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14309..15448)
                     /gene="gfcE"
                     /locus_tag="ACHK07_002030"
     CDS             complement(14309..15448)
                     /gene="gfcE"
                     /locus_tag="ACHK07_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO8102360.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15494..17590)
                     /gene="gfcD"
                     /locus_tag="ACHK07_002031"
     CDS             complement(15494..17590)
                     /gene="gfcD"
                     /locus_tag="ACHK07_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8102361.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17590..18336)
                     /gene="gfcC"
                     /locus_tag="ACHK07_002032"
     CDS             complement(17590..18336)
                     /gene="gfcC"
                     /locus_tag="ACHK07_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8102362.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLAVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18333..18977)
                     /gene="gfcB"
                     /locus_tag="ACHK07_002033"
     CDS             complement(18333..18977)
                     /gene="gfcB"
                     /locus_tag="ACHK07_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO8102363.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19084..19389)
                     /locus_tag="ACHK07_002034"
     CDS             complement(19084..19389)
                     /locus_tag="ACHK07_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102364.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19831..20043)
                     /gene="cspH"
                     /locus_tag="ACHK07_002035"
     CDS             complement(19831..20043)
                     /gene="cspH"
                     /locus_tag="ACHK07_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO8102365.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20329..20541
                     /gene="cspG"
                     /locus_tag="ACHK07_002036"
     CDS             20329..20541
                     /gene="cspG"
                     /locus_tag="ACHK07_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO8102366.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20552..20740
                     /gene="ymcF"
                     /locus_tag="ACHK07_002037"
     CDS             20552..20740
                     /gene="ymcF"
                     /locus_tag="ACHK07_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO8102367.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20715..20945
                     /gene="ymcE"
                     /locus_tag="ACHK07_002038"
     CDS             20715..20945
                     /gene="ymcE"
                     /locus_tag="ACHK07_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO8102368.1"
                     /translation="MHRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            20935..21108
                     /gene="gnsA"
                     /locus_tag="ACHK07_002039"
     CDS             20935..21108
                     /gene="gnsA"
                     /locus_tag="ACHK07_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO8102369.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21156..22229)
                     /gene="yccM"
                     /locus_tag="ACHK07_002040"
     CDS             complement(21156..22229)
                     /gene="yccM"
                     /locus_tag="ACHK07_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8102370.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRYMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(22301..25045)
                     /gene="torS"
                     /locus_tag="ACHK07_002041"
     CDS             complement(22301..25045)
                     /gene="torS"
                     /locus_tag="ACHK07_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO8102371.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTLEVGSCFCLRLPLRVATAPAPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDIPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            25128..26156
                     /gene="torT"
                     /locus_tag="ACHK07_002042"
     CDS             25128..26156
                     /gene="torT"
                     /locus_tag="ACHK07_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO8102372.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQSSTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26129..26821)
                     /gene="torR"
                     /locus_tag="ACHK07_002043"
     CDS             complement(26129..26821)
                     /gene="torR"
                     /locus_tag="ACHK07_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO8102373.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26951..28123
                     /gene="torC"
                     /locus_tag="ACHK07_002044"
     CDS             26951..28123
                     /gene="torC"
                     /locus_tag="ACHK07_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO8102374.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDKLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28123..30669
                     /gene="torA"
                     /locus_tag="ACHK07_002045"
     CDS             28123..30669
                     /gene="torA"
                     /locus_tag="ACHK07_002045"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO8102375.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSKNLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTIAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30666..31265
                     /gene="torD"
                     /locus_tag="ACHK07_002046"
     CDS             30666..31265
                     /gene="torD"
                     /locus_tag="ACHK07_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO8102376.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAMDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(31358..31663)
                     /gene="cbpM"
                     /locus_tag="ACHK07_002047"
     CDS             complement(31358..31663)
                     /gene="cbpM"
                     /locus_tag="ACHK07_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO8102377.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31663..32583)
                     /gene="cbpA"
                     /locus_tag="ACHK07_002048"
     CDS             complement(31663..32583)
                     /gene="cbpA"
                     /locus_tag="ACHK07_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO8102378.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32843..34096
                     /locus_tag="ACHK07_002049"
     CDS             32843..34096
                     /locus_tag="ACHK07_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFO8102379.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQIYSGVSGG
                     ITYTIQYVEDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDD
                     ENISLQPEWINAPGISDLDTGIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILV
                     LKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSD
                     NKIQGEGSLMSWNFLFFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEKRIN
                     EVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPE
                     LFAQAYKLNNKEMNAQLDYLNRYAI"
     gene            34389..35630
                     /gene="agp"
                     /locus_tag="ACHK07_002050"
     CDS             34389..35630
                     /gene="agp"
                     /locus_tag="ACHK07_002050"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO8102380.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(35668..35895)
                     /gene="yccJ"
                     /locus_tag="ACHK07_002051"
     CDS             complement(35668..35895)
                     /gene="yccJ"
                     /locus_tag="ACHK07_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO8102381.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(35916..36512)
                     /gene="wrbA"
                     /locus_tag="ACHK07_002052"
     CDS             complement(35916..36512)
                     /gene="wrbA"
                     /locus_tag="ACHK07_002052"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8102382.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            36885..37058
                     /gene="ymdF"
                     /locus_tag="ACHK07_002053"
     CDS             36885..37058
                     /gene="ymdF"
                     /locus_tag="ACHK07_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8102383.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(37141..38469)
                     /gene="rutG"
                     /locus_tag="ACHK07_002054"
     CDS             complement(37141..38469)
                     /gene="rutG"
                     /locus_tag="ACHK07_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO8102384.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(38490..38984)
                     /gene="rutF"
                     /locus_tag="ACHK07_002055"
     CDS             complement(38490..38984)
                     /gene="rutF"
                     /locus_tag="ACHK07_002055"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO8102385.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(38995..39585)
                     /gene="rutE"
                     /locus_tag="ACHK07_002056"
     CDS             complement(38995..39585)
                     /gene="rutE"
                     /locus_tag="ACHK07_002056"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO8102386.1"
                     /translation="MNEAVSPGALSTLFTNARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(39595..40395)
                     /gene="rutD"
                     /locus_tag="ACHK07_002057"
     CDS             complement(39595..40395)
                     /gene="rutD"
                     /locus_tag="ACHK07_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO8102387.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(40403..40789)
                     /gene="rutC"
                     /locus_tag="ACHK07_002058"
     CDS             complement(40403..40789)
                     /gene="rutC"
                     /locus_tag="ACHK07_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO8102388.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(40801..41493)
                     /gene="rutB"
                     /locus_tag="ACHK07_002059"
     CDS             complement(40801..41493)
                     /gene="rutB"
                     /locus_tag="ACHK07_002059"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO8102389.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(41493..42584)
                     /gene="rutA"
                     /locus_tag="ACHK07_002060"
     CDS             complement(41493..42584)
                     /gene="rutA"
                     /locus_tag="ACHK07_002060"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO8102390.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGYFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
BASE COUNT        10347 a        10887 c        11183 g        10281 t
ORIGIN      
        1 gcgatgaaat taacgcctgg cgtaatgcga tggaagcggc gaactacacg tttgagcaca
       61 atgggcggaa atgggactac gggaagtcaa cgcagacgcg tcttgagccg tcggtggcgg
      121 cagcgaaagc ggggaaactg ccggaggcgt ttttctggac ggatgcggaa aacaatgatg
      181 tgccggtgac agcagaagag cttattgcgc tgagtgaagc ggcagagcag gcgatgttca
      241 ccaaagggat ggaaatccac gtccggcagc gcaccatgaa gaaagagctg gaaaagctga
      301 ccagtgcgga tgagatactg gcatacaggg ttggctgggc acaggaataa cagacaaaaa
      361 gatgggggac catcacctcc cccatgaact gagtcgatta tgtaattatt gtgaagagac
      421 cttacctaca cagataaaga gtgtcacgga aaagttaatc ctgaaagtga tgcaggtctc
      481 atttccagag catcctgttg atatggataa tttttcagat ttccggaagt gagagttttt
      541 ggtgtttcca taccagccac aattgatgtg ttttatggcg cctggcagtg cgattaaggg
      601 aacagggggt aataaaaaaa acgggggtat caccacaccc ccggtacaag aaaggagtaa
      661 ctataatgga aaaattaatg aagaccttta gtgctacgtt atatggcaaa taaagactgc
      721 cacagggaag tttgcagaaa cagtgatgcc gatcgtgttc ctgtgctctt ctctgataag
      781 agtaattttt cagatttccg gaagtccgta ttttgcccgg gcgcggttgc atgcttcatt
      841 cgcgatgcca ttttctccgg acattgcgaa ttcccggcat gtggacggac ggtttttgta
      901 aacagagcaa tatgcgtttt tacccggagt tcccgccaga gcgacacatc ggggattttt
      961 ctgactggtg ccgcgcatac agcgatgaaa gggggatatt tgctcagtga gactgaccgg
     1021 aacctttccg ccagcatcgt cagcttctgc ccagtaaaaa gagacgcgga aaaatgcaca
     1081 acaggcacca cacgtcatgc atggattggg gttattcata attcatcggt atagcgataa
     1141 atcgtaaaga gaacaatgta cctagtgtgt attgagctaa atataccaat tagtatacca
     1201 agtgtatact aagagcaaaa aaaggaaagg ggctaccatt tggtaacccc ttgattgttt
     1261 ggcggaagcg cagagattcg aactctggaa ccctttcggg tcgccggttt tcaagaccgg
     1321 tgccttcaac cgctcggcca cacttccgga atgacgcgca ctataaacat cccgatgcgg
     1381 cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca ttgatagcgt
     1441 gaaataaaag cagattgatc atttcatcac cataaattcc ttctctttta ctcgtttagc
     1501 aactggctaa acatcccacc gcccggcaaa aagaaaaata ggtccatttt tatcgctaaa
     1561 agataaatcc acacagtttg tattgttttg tgcaaaagtt tcactacgct ttattaacaa
     1621 tactttctgg cgacgtgcgc cagtgcagaa ggatgagctt tcgttttcag catctcacgt
     1681 gaagcgatgg tttgccttgc tacagggacg tcgcttgccg accataagcg cccggtgtcc
     1741 tgccggtgtc gcaaggagaa gagacgtgcg atatgaataa cgaggaaaca ttttaccagg
     1801 ccatgcggcg tcagggcgtt acccggcgca gctttctcaa atattgtagt ctggctgcca
     1861 cgtcgctggg attaggcgcg ggaatggcac caaagattgc ctgggcgctg gagaacaaac
     1921 cgcgcattcc ggtagtatgg atccacggtc tggaatgcac ctgctgtacc gaatctttta
     1981 tccgctccgc tcacccgctg gcaaaggacg tcatcctttc cctgatttcc ctcgattacg
     2041 acgatacttt gatggctgcc gccggaaccc aggcggaaga agtatttgaa gacatcatca
     2101 cgcaatacaa tggcaaatat atcctcgcag tagaaggtaa tccgccgctg ggcgagcagg
     2161 ggatgttctg tatcagcagc ggtcgaccgt ttattgagaa actcaaacgt gccgctgccg
     2221 gagccagcgc gattatcgcc tggggaacct gcgcgtcctg gggctgcgtg caggccgcgc
     2281 gacctaatcc gacacaggca acgcctatcg acaaagtcat caccgacaaa cccattatca
     2341 aagtacctgg ctgcccgccg atcccggatg tgatgagcgc catcattact tacatggtga
     2401 cctttgatcg cttgccagat gtcgacagaa tgggtcgtcc actgatgttt tatggtcagc
     2461 gtatccacga taaatgctat cgccgcgctc acttcgacgc cggagagttc gtccagagtt
     2521 gggatgatga cgctgcccgc aaaggttatt gcctgtacaa aatgggctgc aaagggccta
     2581 ccacctataa cgcctgttcc tccacacgct ggaatgatgg cgtttctttc ccaatccagt
     2641 ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg ggatcgcggt tcgttctaca
     2701 gccgcgtggt cgatattccg caaatgggta ctcattccac cgccgatacc gtcggtttaa
     2761 ccgcgcttgg cgtggtggca gcggctgttg gtgtgcacgc agtcgccagc gccgttgacc
     2821 agcgcagacg tcataaccag caacctacag aaaccgaaca tcagccaggc aatgaggata
     2881 aacaggcatg agcactcagt acgaaactca gggatacacc atcaataatg ccggacgccg
     2941 cctggtggtc gacccgatta cgcgcattga aggccacatg cgctgcgaag tgaatattaa
     3001 cgatcagaat gtgatcacca atgccgtctc ctgcggcacc atgtttcgcg ggctggagat
     3061 catcctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc gttgaacgta tctgcggcgt
     3121 ctgtactggc gtacacgccc tggcttcggt ttacgccatc gaagatgcaa tcggtattaa
     3181 agtgccggac aacgccaata tcatccgcaa cattatgctg gcaacgctct ggtgccacga
     3241 tcatctggtg cacttctatc agctcgccgg gatggactgg atcgatgtgt tagatgcgct
     3301 gaaagccgac ccgcggaaaa cctccgaact ggcgcaaagt ctctcctcat ggccgaaatc
     3361 atcccctggc tatttcttcg acgtacaaaa tcgcctgaag aaattcgttg aaggcgggca
     3421 gttggggatc ttccgcaatg gctactgggg gcacccgcag tacaaactgc cgccagaagc
     3481 caacctgatg ggctttgccc actatctcga agctctcgat ttccagcgtg aaattgtcaa
     3541 aatccacgcg gtctttggcg gtaaaaaccc gcatccaaac tggattgtcg gcgggatgcc
     3601 atgcgccatt aacattgacg aaagcggcgc ggtcggggcg gtcaatatgg aacgcctgaa
     3661 cctggtgcag tcgattatca cccgcacggc agacttcatt aacaacgtga tgatccccga
     3721 cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa atcggcacgg ggctttctga
     3781 taaatgtgtc ctcagctacg gcgcattccc ggatattgcc aacgactttg gtgagaaaag
     3841 tctgctgatg cctggcggcg cggtgattaa cggcgacttc aacaatgtgc tgccagtgga
     3901 tttggttgat ccgcagcagg tgcaggagtt tgtcgatcac gcctggtatc gttatcccaa
     3961 cgatcaggtc gggcgtcatc cattcgatgg cattaccgac ccgtggtaca accccggcga
     4021 tgtcaaaggc agcgatacca acattcagca gctgaatgaa caggaacgct actcgtggat
     4081 caaagcgccg cgctggcgcg gtaacgcgat ggaagtgggg ccgctggcac gcacgttaat
     4141 cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc gatcgcatga tgtcggcgct
     4201 gaacctgccg ctttccggta tccagtcaac gttgggccgc attttgtgcc gcgcgcacga
     4261 agcacagtgg gccgcaggta agttgcagta tttcttcaac aagctgatga ctaacctgaa
     4321 aaacggcaat cttgccaccg cctccacgga aaaatgggaa cccacaacct ggccgacaga
     4381 gtgccgtggt gtcggcttta ccgaagcgcc gcgcggggcg ttaggccact gggccgccat
     4441 tcgcgatggc aagattgatc tctaccagtg cgtggtgccg accacctgga acgccagccc
     4501 gcgcgatccc aaagggcaga ttggcgctta tgaagcggcg ctgatgaaca ccaaaatggc
     4561 gatccccgag caaccgctgg agatcctgcg tactctgcac agctttgatc cgtgcctcgc
     4621 ctgttcaaca cacgtgctgg gcgacgacgg tagcgagctg atctccgtgc aggtgcgtta
     4681 acagcgaagg agaatcatca tgcaacagaa aagcgacaac gttgtcagcc actatgtctt
     4741 tgaagcgcca gtgcgtatct ggcactggct gacggtgtta tgcatggcgg tgttgatggt
     4801 caccggttac tttatcggca aaccgctacc ttccgtcagc ggcgaggcga cgtatctgtt
     4861 ctatatgggc tacatcaggt taattcactt cagcgccggg atggttttta ccgtggtctt
     4921 gctgatgcgg atctactggg cttttgttgg caatcgatac tcccgcgagc tgtttatcgt
     4981 gccagtatgg cgtaaaagct ggtggcaggg cgtgtggtat gaaatccgct ggtatctgtt
     5041 tctggcaaaa cgtccgagtg ccgatatagg ccataacccc atcgcccagg cggcgatgtt
     5101 tggctatttc ctgatgtcgg tctttatgat catcaccggc tttgcgctgt acagcgaaca
     5161 cagccagtac gctatttttg cgccgttccg ttatgtggtg gaatttttct actggacggg
     5221 aggcaactca atggacattc acagctggca tcggctgggg atgtggctga ttggcgcgtt
     5281 tgtgatcggt catgtctata tggcgctgcg tgaagacatc atgtccgacg acacggtgat
     5341 ctccaccatg gtcaacggct accgtagcca caaatttggc aaaataagta acaaggagcg
     5401 ttcatgagcg agcaacgcgt ggtggtcatg gggctgggca acctgctgtg ggccgatgaa
     5461 ggtttcggcg tgcgggtggc ggaacggttg tatgcccatt accactggcc cgagtatgtg
     5521 gagattgtcg atggcggtac tcagggactg aacttgctgg ggtatgtcga aagcgccagc
     5581 catctgttga ttctcgatgc cattgactac gggctggaac ctggaacgct gcgaacctat
     5641 gccggagaac gtattccggc ttatctcagc gcgaagaaaa tgagcctgca ccagaacagt
     5701 ttctccgaag tgctggcgct ggcagatatc cgcggacatc tgccagcaca tattgccctc
     5761 gtcggtctgc aacccgcaat gctcgacgac tacggcggca gcctgagcga actggcacgg
     5821 gagcaactgc ccgcagcgga acaggcggcg ctggcgcagc tgggtgcatg gggaattgtg
     5881 ccgcaaccgg ctaatgaatc gcgctgtctc aattatgact gtctgtcgat ggaaaattac
     5941 gaaggcgttc gcttgcgcca gtacaggatg acactggagg agcagggatg agcaacgaca
     6001 cgccatttga tgcgttgtgg caacgaatgc tggcgcgcgg ctggacgccg gttagtgaat
     6061 tccgtcttga cgactggctt acgcaagcgc cagacggcgt ggtgttatta agcagtgacc
     6121 cgaaacgcac gccagaggtc agcgataatc cggtaatgat tggcgaatta ctgcgcgagt
     6181 ttcccgacta cacatggcag gtggcgattg ctgaccttga acagagcgaa gccatcggag
     6241 atcgctttgg cgtctttcgc tttcccgcca cattagtgtt taccggcgga aactatcgcg
     6301 gcgtgctgaa tggtattcac ccgtgggcgg aactgataaa cctgatgcgt gggcttgtcg
     6361 aaccacaaca ggagcgtgcc tcatgagcga aacttttttc catctgctgg ggccaggaac
     6421 gcaaccgaac gatgacagtt tcagcatgaa tccgctgccg atcacctgtc aggtgaatga
     6481 tgaaccgagt atggcggccc tggaacagtg tgctcacagc ccgcaggtga ttgcgctgtt
     6541 aaacgagtta caacatcaac taagcgaacg ccagccgccg ttgggcgagg tgctggcagt
     6601 cgatctgtta aatctcaatg ccgacgatcg tcactttatc aatacgcttc tcggggaagg
     6661 ggaagtgtca gtgcgcattc agcaggctga cgacagcgaa agtgaaatac aggaggcgat
     6721 cttctgcgga ttatggcggg tgcgcagacg tcgcggcgaa aagttgctgg aggacaaact
     6781 ggaagctggc tgcgcgccgc tggcgttgtg gcaggcggca acgcaaaatc tcttgccgac
     6841 agattcgctg ttaccgccgc ccattgatgg cctgatgaat ggcctaccgt tggcgcatga
     6901 gttactggcg catgtacgta accccgacgc gcagccgcac agcattaatc tgacgcaatt
     6961 acccatcagc gaggctgatc ggctttttct ctcacgtctc tgtgggccgg gaaatattca
     7021 gattcgtacc attggctatg gcgagagcta tatcaacgcc acggggttac gccatgtctg
     7081 gcatttacgc tgtacggaca ccttaaaagg cccgttactg gaaagttatg aaatctgccc
     7141 aataccggaa gtggtgctgg cagcgccaga agatttggtc gactctgcgc agcggcttag
     7201 cgaggtatgt cagtggctgg cggaaggtgc accgacgtaa aaagacggta agtatcgctt
     7261 tcagtcttat gaatatcgca atcggcgaat acctctggtc gtagagtttc aggataaaga
     7321 gggagatcta ccattatcgg gttatttttc tctcttcgcc tacaggagtg cgcatgtggg
     7381 atgtcattga tttatcgcgc tggcagtttg ctctgaccgc gctgtatcac tttttatttg
     7441 taccccttac cctggggctg atttttttgc tggctatcat ggaaaccatt tacgtggtca
     7501 ccggcaaaac aatctaccgc gatatgacgc gcttctgggg taagctcttc ggtatcaact
     7561 ttgctcttgg cgtggctacc ggcctgacca tggagtttca gtttggtact aactggtcat
     7621 tctattccaa ctatgtgggc gatatttttg gcgcaccgct ggcgatggaa gcattaatgg
     7681 ccttcttcct cgaatccacc tttgtcgggc ttttcttctt cggctggcag cggctgaata
     7741 aataccagca cctgctggtg acgtggctgg tggcgttcgg ttcaaatctc tctgcattgt
     7801 ggatattgaa tgccaacggt tggatgcaat acccgaccgg tgcgcatttt gatatcgaca
     7861 ccctgcgtat ggagatgacc agcttcagcg agctggtctt taacccggtc agccaggtga
     7921 aatttgtgca caccgtaatg gcgggctacg tgaccggggc catgtttatt atggcgatca
     7981 gcgcctggta tttactgcgc ggacgggagc gcgatgtcgc attacgctcg tttgccatcg
     8041 gttccgtctt cggtactctg gcgattattg gcacgctgca actcggagac agttctgcgt
     8101 atgaagtcgc gcaagtacaa ccggtaaaac tggcggcgat ggaaggggag tggcaaacgg
     8161 aacctgcacc tgcaccgttc catgtggttg catggccgga acaggatcaa gagcgtaacg
     8221 cctttgccat caaaattccc gcgctgctag ggatcctcgc cactcactca ttagataaac
     8281 ccgtgccggg tctgaagaat ttgatggctg aaacctaccc acgcttgcaa cgcggacgta
     8341 tggcctggct gttaatgcag gaaatatcgc aaggcaatcg tgagccgcat gtgttgcagt
     8401 cattccggga gctggaaggc gacctgggct acggcatgtt gctctcccgc tatgcgccgg
     8461 atatgaatca tgtcacagcc gcacagtacc aggcggcgat gcgtggcgcg atacctcagg
     8521 ttgcgccggt attctggagt ttccgcatca tggtgggctg tggttccctg ctgctactgg
     8581 tgatgcttat tgcgcttgtc cagacgctgc gtggcaaaat cgaccagcat cgctgggtgc
     8641 tgaaaatggc gctctggagt ttgccgctgc cgtggattgc gattgaagcc gggtggttta
     8701 tgaccgagtt tggtcggcag ccgtgggcga tacaggacat cttaccgaca tactccgcgc
     8761 actccgcttt aaccacagga caactggctt tctcactgat catgatcgta ggcctttaca
     8821 ccctgttctt aatcgccgaa gtctacctga tgcagaaata tgcccgtctg gggccgagcg
     8881 cgatgcagag tgaacaaccg acgcagcaac aggggtaaag gagaaaatca tgtttgatta
     8941 tgaaacattg cgcttcatct ggtggctgct gattggcgtg atcctggtgg cctttatgat
     9001 ctccgacgga tttgacatgg ggattggctg tttgctgccg ctggtggcgc gtgatgatga
     9061 tgaacgccgg atagtgatta atagcgttgg tgcgcactgg gaaggcaatc aggtctggtt
     9121 gatcctcgca ggtggggcat tatttgccgc ctggcccaga gtgtatgcag cggcgttttc
     9181 cggcttttat gtggcgatga tcctggtgct gtgctcgctg ttcttccgcc cgctggcctt
     9241 tgattatcgc ggaaaaatcg ccgatgcacg ctggcgtaaa atgtgggatg ccggtctggt
     9301 catcggcagt ctggtgccgc cggtagtctt cggtatcgcc ttcggcaact tgttgctcgg
     9361 cgtgccgttt gccttcactc cgcaattacg cgtggagtat ctcggcagct tctggcaact
     9421 gctgacgcca ttccctttat tgtgcggatt gctcagcctt gggatggtga ttttgcaagg
     9481 tggcgtctgg ttacaactga aaactgttgg tgtgattcat ctgcgttcac agctggcgac
     9541 taaacgcgct gcactgttgg tgatgctgtg ctttttgctg gcgggttact ggctgtgggt
     9601 cggtattgat ggctttgtac tgctcgccca ggatgctaac ggtccttcca atccgttaat
     9661 gaaactggtg gcagtgctac ctggtgcctg gatgaataat tttgtcgagt cgcccgtttt
     9721 gtggatcttc ccgctgctgg ggttcttctg cccattgctg acggtgatgg cgatttatcg
     9781 tggtcgcccg ggttggggat ttttgatggc gtcattgatg caatttggcg tgattttcac
     9841 ggcaggcatc acgctgttcc cctttgtcat gccgtcaagc gtgagtccaa tctccagcct
     9901 gacgttgtgg gacagtactt ccagtcagct gacgctgagc attatgttgg taatcgtgct
     9961 gatatttttg cccattgtgt tgctctacac tctctggagc tactacaaaa tgtggggacg
    10021 catgacgaca gaaactctcc gccgtaacga aaacgagctg tactaaggag caggaacaat
    10081 gtggtattta ctttggttcg tcggcatttt gttgatgtgt tcgctctcca cccttgtgtt
    10141 ggtatggctg gacccgcgat tgaaaagtta acgaacgtaa gtctgatgcg gcgcattagc
    10201 gtcgtatcag gcaatcaata atgtcggata tgaaaagcgg aaacatatcg atgaaagcga
    10261 tcttgatccc atttttatct cttctgattc cgttaacccc gcaatctgca ttcgctcaga
    10321 gtgagccgga gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt catggtgtgc
    10381 gtgccccaac caaggccacg caactgatgc aggatgtcac cccagacgca tggccaacct
    10441 ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc tatctcggac
    10501 attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc tgcccgcagt
    10561 ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca ggcgaagcct
    10621 tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca gatacgtcca
    10681 gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat aacgcgaacg
    10741 tgactgacgc gatcctcagc agggcaggag ggtcaattgc tgactttacc gggcatcggc
    10801 aaacggcgtt tcgcgaactg gaacgggtgc ttaatttccc gcaatcaaac ttgtgcctta
    10861 atcgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg gaactcaagg
    10921 tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg ctgacggaaa
    10981 tatttctcct gcaacaagca cagggaatgc cggagccggg gtggggaagg atcactgatt
    11041 cacaccagtg gaacacgttg ctaagtttgc ataacgcgca attttattta ctacaacgca
    11101 cgccagaggt tgcccgcagc cgcgccaccc cgttgttgga tttgatcatg acagcgttga
    11161 cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca gtgctgttta
    11221 tcgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc aactggacgc
    11281 ttccaggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa cgctggcgtc
    11341 ggctaagcga taacagccag tggattcagg tttcgctggt cttccagact ttacagcaga
    11401 tgcgtgataa aacgccgctg tcattaaata cgccgcccgg agaggtgaaa ctgaccctgg
    11461 caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggccggtttt acgcaaatcg
    11521 tgaatgaagc acgcataccg gcgtgcagtt tgtaatgtat aaaaaagagc attcagctac
    11581 ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac cggaaaaggc
    11641 gttcacgccg catccggtca ctttaccttc agttttactc tttctcggag taactataac
    11701 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    11761 tagcgccttt aatattgacg cctgcctgtt ccagacgctg cattgacaaa ctcacctctt
    11821 tggcggtgtt caggccaaaa cgtgcaacca gcaggctggt gccgacagag cgccccacga
    11881 ccgcagcatc gctcaccgcc agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt
    11941 cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca gacgggttag
    12001 gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc tggatcactt
    12061 tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta ctcacggtaa
    12121 acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact ttttgatcgg
    12181 actgggcgat caccgctgcc agagttgaac tgacaaatgt tttaccactg tctggcgtcg
    12241 caccggtgat catcagaatg ttattctcag tctccatcat agcgaaatgc agactggttc
    12301 gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc aggaagggga
    12361 tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc agacgggtgc
    12421 gtttatccag ccactcggac attgggatag tggcataaac gctgatgccg tgctcttcca
    12481 gttgttccgg tgcttctaca ccacgacgca acatcgcacg cgccagcacg gcacccaccg
    12541 aaataaacag gccaagaata aaaccaagca ccacgttcaa cgcttttttc ggtttcactg
    12601 gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca ctggatttcg
    12661 aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg cccgcttcta
    12721 cgtcacgact taaacgcaac acttcctgtt gggtggaagg catagccgat acccgcttat
    12781 tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg
    12841 ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt
    12901 gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta aggtcaaccg
    12961 aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca
    13021 cttcaggcaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag
    13081 cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgggta atcaactggg
    13141 gatcatcacc ggtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg
    13201 taaaggtttc ctgcaacgcg ttaatcgctt ccagttcggt acgttggctc aggacaaact
    13261 gtgttcctgg tttggcctta atatccgcta tagtcagcgc aacgccatct ttttccagac
    13321 gctggccgac cataccattg acggtgaact cttcaccttc cagcgtatag tggccgtttt
    13381 ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc
    13441 tgatcgccag ctcacctggt ttctctttgg ttaatctcgc ccagccgcga cccacaatcg
    13501 gaaaatactt ctgctcaacc atgtcgcgca gattcagttc agcaatggtt ttaccgagaa
    13561 tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca
    13621 tatcactcag gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat
    13681 ctgcctgata aattggtgtg cttaacagcg agtaagcgac agcgatcagc gtgaataacg
    13741 cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga cgaagcagat
    13801 cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat atttttagtt gtcatcgtat
    13861 tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc gcgctccaac
    13921 ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg gatctctttc
    13981 tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc gattgcggta
    14041 acctgagcaa tatgttccga ctccattgcc agaatcagat cgtaatttcg tgccatctca
    14101 gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc ggcaacatct
    14161 gccgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga tttgactttt
    14221 acgcccggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg
    14281 gtacaaacca ccaggattga gttaaatttt agttgggcca tgtttgaatc caccgtgaag
    14341 tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc acacggttcc
    14401 aacgagccag aggcgcggta gtgacataaa caatgtcgta aggttcaagc tggaattccg
    14461 ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg gcgattttgc
    14521 cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca gcgacatcct
    14581 gattcatacc ctcagcgtta cccagcgctt ccgccagggt cataccgctg cggtccattt
    14641 tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca tcgttgcgcg
    14701 gaataaataa aatatctccc gggtgcagca gtttgttctg gcgcaagtcg ccacgctgca
    14761 tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc acattgcgcc
    14821 agtctgcatc tgcggtcaga ccgcctgcgg cgttaatcgc atccatgatg gtcagaggaa
    14881 tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg
    14941 atcggaacgc cgcgacgctg acatcgacct gcgggctttc aatgactgaa tccagacgcg
    15001 ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg cgaccaatat
    15061 aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg gcgctacggt
    15121 attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc aacacatcac
    15181 caatgccgat acgatattca tagttcgcga tttgctgctc cagctccggg ttagcctgcg
    15241 acataatcgg tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga tagatattga
    15301 ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg ttactggtcg
    15361 ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc aatgtcagta
    15421 ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat gtaatgttat
    15481 gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt ggatagagct
    15541 ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg
    15601 cgcggttgcg gttaggtcca atggtcatca agtcgaacgg aatcgagata taaaaacctt
    15661 tactaaagcc gccttcgccg tagtcatctt tagatacgtt actgattgcc gcccataccc
    15721 ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg
    15781 ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc gggttccagt
    15841 aagccgtcac gaaacctgtg ggcgtggaat aatcggtgaa gcgcatcatg ttgtcccagt
    15901 cacgttgctt cacgtagtta acgtccacac ccagcgccca gctggcatct agcgggcgat
    15961 aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag ataaccgcca tacacctggc
    16021 catagaaacc attgcctaag tcggcaaagt agttcgcctg caagttgttg agataaacgt
    16081 cattgcgaac gtagtcacgg atatgcgtgc gcacgcgggg cagggtagag tccgcgggca
    16141 acagtgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata ccgccatcaa
    16201 gcagcaggtg gtcggtaaac cagtagcggg cgctggacat cagccccagc tggaacatat
    16261 agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag tagctaaagc
    16321 gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttcagct tcaacacgtt
    16381 gttgttgcag tggctctgat tgaccaggcg ctgtacctgc cagctgtttg cgcaggctgg
    16441 ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc acgctaatct
    16501 tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg accgcttcac
    16561 gagagtcacg gtatttgtat tgctgaccag acatatacag cgtcttatcg cgtagctgaa
    16621 tttctggcgc gtcaaagccc gcgttatact tcagggcggt aagttggttt gctaccgtgg
    16681 tgtactgcaa tccttcagat tcaggcgcag gttgatatgc cggttttggc gtatcgcgca
    16741 gggcaggacg cagatcgttg aaattggtcc gtaaagtgaa accaaacatc aacgtgttac
    16801 cgcgttcata actcaggttg aggtctgccc agctggcagc gcgataaact gcaccgacgt
    16861 tgaaatggct tgcctgaggc agtttgccag cgaaatcatt ctggtaattg ttgccgtcgt
    16921 attcgagttt cagacgcagg ggattccacg gcgtttgata ctcaatgccg ccaaagatgg
    16981 aagccgggcc acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga gactctgcgc
    17041 gatgacaata tttatcgctc acccggcaaa acgggttggt aatattgccc gcattaccgg
    17101 cgtagcccca tgccatcccg agggtgaaat caaatggccc cgcttgcttg ctggccacca
    17161 aatactcacc gtcaaacaga cccgtaccag caatatcacg tttaccaaac gccacttgcg
    17221 gtagccagta accttcttcc cacaggcgaa gtttaaaatc gaatgatttg tctttgtatg
    17281 actgatcgcc gctgaaatct tcccactggc tatatttacg tgtgcgcaca tccgtataac
    17341 gaatggtgcc ttccagccat gggaacagcg ccacggaggt ggagtagaaa cggtattgat
    17401 cgttatcccg gtagttgacg ctgaattcac cttccggtgc gatgcgcgca tttggcatct
    17461 gcaacaatcc tgtgccgcca aagtccgatt gcgacgaacc cagcggatcc gggtaggtta
    17521 atacttcagc atgacaggca ctggagacgg caatggccag gcagcttaaa agataagaat
    17581 tcttcttcat cagtcaggaa cacgttgcgt taaaacagac acaatctggt catttaaatc
    17641 agcatatttt tcgggcagta catgcgcaga aaagcctaac caaagctggc tgccaggcgg
    17701 tggttccaca tgtcttttat tccacagggc aacaggggcg acgacagtct caccctcagg
    17761 cgtaatcacg ataacgttat tcttgtccgc gcctgcaaga cgggggtgat cttgcagata
    17821 atcggtaacg ctacggcctg cctgccaggg caattgccca gccccagaaa ccgcacccag
    17881 cagagtaata gtcaccggac gctgtacggt atagagcgta tagtccccga ccagcggggg
    17941 attgctgttc tcatccactc gtacaaaatc agggtccagt ttgaccgcta agcgaccagt
    18001 gatattgaga ttgagtaact gctggcgcac ggatttaatc gttgccgcca catcgtcgtc
    18061 agcttctgct tcccaggaag caagctgtgc catgacatgt tggtagtctt taagcgcttt
    18121 agctttcgcc gcagagtcgg tcagcaaggc accaggccac cagaggcgat cgcgcagttg
    18181 cggttgtgtc accaactggg caacattttc cacggggcca acggaaagtg tctgttgttc
    18241 gccaggcaga taaatagtca ccgttccttg cgcaaaggca tggggtgtca ttacgtaaag
    18301 tacgctggca atgaaatacg actgtaattt attcatggtg cgggtttaag aaacgtcatt
    18361 tctacgggaa taacccctgc gcctaacatt tgccgacttt gacgaacctg gccagtagcc
    18421 gaatcgaccc agaatgtgtt ttgccactgt ttttctggca gcgtggaagt cacctcttcc
    18481 tgccagacat tacacagcac atcgcgaccc gcaatatgca gcgtctctaa tccagcaaag
    18541 gaaaaagtgg aactcagggt cgcagagcga aaatggttgt cttcactcca gagaatatcg
    18601 cgcgtccagc ggctgccgtc ttttattgcc agcgcgttac gcagagggtc ctgcccggaa
    18661 tttgtcactt ccagcagatt attattaagt ttgacggtct ttaaaagacg tccattgtgg
    18721 gtaaccagca tggcgttatc ctgggataac catttgcttt gttcttgttc tatatatccc
    18781 agtaccacaa agattcgttg cccttcatta aggcgtaaat acatcgtgga ataaggcaac
    18841 gcctggatct gctgatccgc tacggtgata tcctgattat cgaaaaggct ggcgcgaaat
    18901 gtatcgacca tactttgctg gctgtgcgta catcccgcaa gaaatagtgc gaaaatcgat
    18961 aaaataagag ggcgcacctt taatccttga aaggttaatt aaaaaaatgc aaatctctaa
    19021 agcccggtac gaatatcgca accgggtatt atttaacttt aatttcgacg cgtttaagca
    19081 gaattagcgc gtagtggacg tggtggttga ggtgttatga gaaccgccat cgccactgtc
    19141 atttgctgca actactgcca acgtcgccac accaacagcc gttgccacac cgacaactgt
    19201 cgccgtgccg acagcgccag cagccacgcc accagtggta gcagccgtcg tggttgcacc
    19261 ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt ttgctgcttc
    19321 aggggcagca aacgcagctg gcgtcgtcaa catgaaggcc ataaggattg cagaaagctt
    19381 gtgtttcata acttttcctt tattcatcgc atggacaata cgtacgcttg agatcaggat
    19441 tgtctacttt gagtataaca tccttgtgaa atctcccttt cctcatacca cttgcactat
    19501 atacagcggg tgagaaaggc gcgcagaggg aaatataaga ttgttaacta ttcccctctg
    19561 gaaatacgac ttgtgagcat aagataaatc ttagcaaaat acaaattaaa gataagaaaa
    19621 ttgttaagaa agctattaac tattattggc aatatagttt caagtggtac tataactcgc
    19681 tgttaagtgg catttataac tattagttgt agagatattc ggttaaatgg attagttttc
    19741 cggtgaaaaa gtaacttgtg ttataataca tggtgttgac tatttaacta tgccaataaa
    19801 accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg ccgctgttgg
    19861 tcctcgcagg ccatttacgc ggcaaaattc cacgcgtaat cctggtataa gcacttctgc
    19921 gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt cggaggggat
    19981 aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca ttttacggga
    20041 caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata aaataaagcc
    20101 agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatctca attatcatgc
    20161 gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac taaagcagtt
    20221 ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta agtagtttct
    20281 gtattaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat gtctaataaa
    20341 atgactggtt tagtaaaatg gtttaacgca gataaaggtt ttggctttat cactcctgat
    20401 gatggcagca aagacgtttt cgtccatttc accgccatcc agagcaatga attccgcacg
    20461 ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg ccccgcggca
    20521 gcgaacgtag ttacgctcta aggttgccgt tattactcaa catctccatt ttcgctgtcc
    20581 atgttgtcat ggttcacagt accgcacatc ggcattcgat gtgacggagc gaaacccttt
    20641 gggcgctaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg tcgcgctaca
    20701 aatacgcact gaccatgcac cgctggattt cacaaaataa tatcaggctc cctcgtggag
    20761 ccttttttat atctgcctta tttttcttca acgctgtatg tatagtaagc gataacctgt
    20821 tgattattga atctttcggg gagatggctt ataacatttc ttacctgacc agggtaccgg
    20881 gaaccaacac cttactggcg tgttgctgtc tttcaagacc agacgaggtt aacagtgaat
    20941 attgaagagt taaaaaaaca agccgaaacg gaaatcgccg actttatcgc gcaaaaaatc
    21001 gccgagctga acaagaatac agggaaagaa gtctctgaaa ttcgcttcac cgcacgagaa
    21061 aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa taatgtgatt ttgtgaacat
    21121 cacccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat tggcgcttgc
    21181 ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc cgcatacatt
    21241 gcgtaaccaa tcaccgcaaa cataatcccc agaaccagta gcgtcattaa ccagccagac
    21301 aacgcaaagg cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa tgttgaggct
    21361 accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg tacctgaatt
    21421 aatttatcga ccgggatccg tgatgggcaa ttttttgcgc atttgccaca gtcgatacaa
    21481 ctttcggcat tgcgacgaat cttaaacggc gacaatagcg aaaccacgcc catcagcgcg
    21541 ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc aatcagcaaa
    21601 acggtcacgc tgattaatgt cgcggtcccc atataacgaa agaaatcgag cattttaacg
    21661 tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc gggcatcaat
    21721 aacgcgatat agagaaaaaa actcaacagc aaatacttta caccgcgcag aggaatatcc
    21781 agccagcggg gaaggacaaa ttgccgacca aaaagtttgt taccgagagc gccgattaat
    21841 tcagaaagcg taccaaccgg gcataaccat gaacaaaagg cctttttgag taatagactg
    21901 atgacgataa aagcgaccaa taacagcatt gcggcggcgt ggacggacgg taactgacct
    21961 gttgcaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc ttcgatacct
    22021 cccggtctgg cgacaaatgt cgtactacct gccgtttcgt aatagcgcac ccaataccag
    22081 aacgtgatgg caatataaat attcattgcc aatagtaaga attgcgtcgc tttacgccag
    22141 gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcgtgcc tggccgccgc
    22201 tgccagcggg ttcttttttt ctctgccatg attttgccag tccgttaagt tgcataccaa
    22261 atgccactat tctagttatt cttaactggc tgatattgat tcaaatcgcg ttcaggtctt
    22321 tcttattcaa ccatgcttcc agagcggcaa cactgcgtgt aatttcttcg tgtggaaggg
    22381 gggcagataa tggctgctgc tccagttgtg cgcatagctg gctggcgctg cgcattccca
    22441 gactcgagca actgcttttt agctgatgcg cggcacgctt tattttctcg ctgtcctggc
    22501 tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc atgttgttta aataatgcca
    22561 gccattcgtg gatcttctcc gtccccatta attgagcatc ttcattgagt tgcgatacat
    22621 caagtggtat atcgttattg acttgcagtt ggagatagtg ctccagtaac tgaccgagca
    22681 cttcacgcgg caccggttta gggataatcc cgcgaaatag tgaactggta cgctggcgca
    22741 gcgtttcgtc aatgacatga gcgctaaagc caatcaaaac cagcgacgga tattgctgtg
    22801 ccagttgccg ggcaagggta atgccgtcga tatccggcag atcaaaatcc accagtgcgg
    22861 cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg ccaatagcaa
    22921 caacctgcgc accactggtg ttcagcatct caacggtaat tcgctgggtt agcgggttat
    22981 cttcaattaa cagcaaacgt aaaccgtcca gacgcaccgc ctgattggct gtttttggcg
    23041 cgggtgctgt ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca acctccagcg
    23101 tgctggtggc gctcagttcg ccgcccatcg cctgggccag acggctactg atagtcagcc
    23161 ccagcccggt gccgccgcgt ttgccactta cctgaataaa tggctggaag atttctgcca
    23221 gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat tgctcaccat
    23281 cagtacggct acgtaaaacg atctgccctt cgtcagtaaa acgcagggcg ttgctcaaca
    23341 ggttggtaat gacctgacga atacgccgcg gatcgcccat taacgcagtc ggtacatcgt
    23401 cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccactc attaattgca
    23461 gggtactttc cagcagcggt cgcggttcaa agggttcatc gctgaccgaa acattcttgc
    23521 cgcctgcttc gatagccgaa taatcgagaa tatcgttcag gatggtcagc aacgattcgc
    23581 cagagtcggt aatcgcccgc aaatcatcac gctgggcgtt aagtgcgggg ttatctgcca
    23641 gtagctgtgc ggtgccgaga ataccgtaca gcggtgtgcg gatctcatgg ctcatcgccg
    23701 ccagaaacgc cgattttgcc tggctggctt tttctgcttc cgcccgtgcc tgtcggtgtt
    23761 ctatcaccag ttcctgcaat tcagctgtac gtgctttgac ctgcgccgcc agctgttcgc
    23821 ggtggcgatt cagtgcatga acactgctgc gaaacgcatc catcagccgc ccgatggtat
    23881 ccagctcccg tacgccagcg gtttccggaa aaggggagtc aatatcaccg tccagcagcc
    23941 gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa accacgcgcc
    24001 agaggatcag aatcagtgcg caaagtgaaa ccatccccag caataacagg ctgtattgcc
    24061 cgcgtgcact ggctttttcc agatgcgcca gtccatgctg attgcgcagc tcaatggtgt
    24121 cgaccagctg actgacttcg ctactaaact gcgcgaactg ggcgatatta ttttgggcga
    24181 gagtttgcag gtgattgctg atttcactgt cctgctggaa cagcgccagc aaatcgctat
    24241 attggctaac ggtagttaac gttgttgcga cctgagcacg aactcccgga tcttcaatgc
    24301 gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc agcgttggcg
    24361 catttttctg gatctgctcc agccccagat tcatcaccat ttgctgcacc cgcagagcgc
    24421 taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc agccgatcga
    24481 gtgcactttc agcagcctga cgctgatctt gttcgatcaa atcgtaaatc ccggcctggg
    24541 tcgctccggc ggaggttgcc gcattattcg cctgaccttg cgccaggcgt gcgatctcat
    24601 cggcggcagc gactatctgc tgactgagtc gctgttgttg ctggcgcagt tgcaaacgct
    24661 gccccaccag ttccccttgc tgacgtaacg aacgggagat ctcctgctcc tgttgttcaa
    24721 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
    24781 tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
    24841 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
    24901 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
    24961 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
    25021 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaat gttgagtcgc
    25081 aaagaggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
    25141 tatttttact tctttccctt ttcatgttgc cggcattttc ggctgataac ctgttgcgct
    25201 ggcatgatgc gcagcatttc acggtgcaat cctctacgcc gcttaaagcc aaacgcgcct
    25261 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
    25321 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
    25381 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
    25441 ccattttgct cggtagtagc acgacgtcat ttcccgatct gcaaaagcag gtagcaagtc
    25501 tgccggtgat cgaactggta aatgctattg atgctcccca ggtgaaaagc cgcgttggtg
    25561 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
    25621 aaccactgaa cgtgctgttg atgcccggac ccgataacgc cgggggcagt aaggagatgg
    25681 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
    25741 gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa cgccatccag
    25801 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggg gaagggcgta
    25861 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
    25921 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
    25981 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
    26041 cgattttagt tctgacgccg aaaaatgccg accgcgaaca tatccgccgc tcgctgtcgc
    26101 cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
    26161 cgccatgttg cgtcaccagt aaatccgcgc tgagtttatg gcgtaaacga cgaattaaca
    26221 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agagagcata cgaagcagac
    26281 gttcacggct gagaatttct cccggattgg tcacaaatgc caccaacatt tcatactctg
    26341 cgcgggtcag tttaatcggc tcaccatccc gctccagcgt atggcgcgac acattcaggc
    26401 aataacccgc aaagcgatag cagttgtcct gagtgagcgg ttgaacttgt cgcgcgaggt
    26461 cgattcgcca gagcagattt tttacccgta ctaccagttc gcgcagttcc agcggtttgg
    26521 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
    26581 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
    26641 cattttcatc gggcaagttg atatccagca gaattaaatc caccggctga ttctgcataa
    26701 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
    26761 aggattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg tgatgtggca
    26821 tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag ccattcatcc
    26881 tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc ataattttac
    26941 aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt
    27001 actggcgctg gtcgctattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt
    27061 tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt
    27121 gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg
    27181 ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgtaaac tggaagcgag
    27241 caacgatatc taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc
    27301 caaacgcgcg gaacttgccg agcgtgaatg ggcacgaatg aaagaaaaca actcggcaac
    27361 ctgccgctcc tgccataact acgacgcgat ggatcatgcg aagcagcatc ctgaagcggc
    27421 acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat
    27481 tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc
    27541 cggtgctaat gacagtggcg acaagctgta ctctattgat attaagccga tttatgcggc
    27601 gaaaggcgat aaagaagcct ctggttctct gctgcctgcc tcggaagtga aagtccttaa
    27661 acgtgacggc gactggctgc aaattgaaat taccggctgg acagaaagcg ccggacgtca
    27721 gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt
    27781 gcagcagcag gtgaaaacgc tggagaaaac caccgtagcc gacaccaata ccgagtggag
    27841 caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat
    27901 ctgggcttat gcggattcgc tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga
    27961 aatctcccac tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac
    28021 cagcctcgat aagcgtgaag aacgcacctt gttgaaatat ctgcaaatga atgcgtctga
    28081 caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc
    28141 tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt cgccgggatg
    28201 ctggggccgt cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc gactgaggct
    28261 gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgacggtg
    28321 aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg
    28381 attgccggat tgccggatca tgtacacaac gcggcgcgta ttcgttatcc gatggtacgc
    28441 gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt
    28501 gtgcgtgtga gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag
    28561 aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc
    28621 cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg
    28681 ggcggagatt actctactgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcgatg
    28741 gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg
    28801 ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat
    28861 gtttatgaat attacgcgca gttgaaagcg aaagtcgccg ccggtgaaat tgaggtcatt
    28921 agcatcgatc cggttgttac atccacccat gagtatctgg ggcgcgagca tgtgaagcac
    28981 attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcgta tacgctgtac
    29041 agtaaaaacc tgtacgataa aaacttcctc gctaactact gtgtgggttt tgagcagttc
    29101 ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa
    29161 ctgaccggca ttgatgccga aaccattcgt ggactggcgc ggcagatggc ggcgaacaga
    29221 acgcagatta ttgctggctg gtgcgtacag cgtatgcagc acggtgaaca gtgggcgtgg
    29281 atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggctttggt
    29341 tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt
    29401 ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa aggctacagc
    29461 agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac
    29521 tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc cggaactaac
    29581 ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa gctggaaacg
    29641 gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat cgtgctgcct
    29701 gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc
    29761 attatcgcca tgaaacaggt cgtgccgccg cagttcgagg cgcgcaacga ctttgatatt
    29821 ttccgcgagc tgtgccgccg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa
    29881 atgggctggc tgaaacgcat ctggcaggaa ggtgttcagc aaggcaaagg acgcggcgtt
    29941 catttgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg
    30001 cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg
    30061 ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta cgacgattgt
    30121 caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc cggctcgcag
    30181 acgtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt
    30241 gagtcggaaa ctctgcgtca gcaatatacg atagcgggta aagagccagt gttcattaac
    30301 ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct
    30361 cgcggtcagg tgttggcagg ggcagtagtt tctgatcgct atgcacccgg cgtggcgcga
    30421 attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa
    30481 tacggtaacc ccaacgtgtt gacaatcgac atcggtactt cgcagctggc gcaggcgacc
    30541 agtgcgcaca ctacgctggt ggaaattgag aagtacaacg gaacagtgga tcaggtgacg
    30601 gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg
    30661 aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt
    30721 tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggctg
    30781 aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcgatggat gagctggaaa
    30841 accgtgttgc cactctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt
    30901 gcggcctgtt tctgatgact gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac
    30961 aggacgagca agaaattaaa cgcttgttag ttgaggcagg gatggaaacc agcggcaatt
    31021 tcaacgaacc ggcagatcat ctggcgatct atctcgagtt gttgagtcat ctgcattttt
    31081 cgctgggaga ggggacggtt cctgcgcgaa gaatcgacag tttacggcaa aaaacactca
    31141 cggcgctgcg gcaatggtta ccagagtttg ttgcgcgttg ccatcagtat gacagctttg
    31201 gtttttacgc ggcactaagc cagttattgc tggtgttagt tgagggcgac caccaaaaca
    31261 gataacgtcg tttgttggcc tgataagacg cgtttagcgt cgcatcaggc attatggggc
    31321 agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg
    31381 aaagccgctg gcgcagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg
    31441 tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgcaccgcgc
    31501 gttgcaccac gatggctgca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg
    31561 gttcaaccac ccccaaaccg acaatttcat tcagttcctc ttcagagatg ccggtatgca
    31621 ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc
    31681 tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacaacg cggcagtgtt
    31741 ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt
    31801 tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgccaggcgg
    31861 gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc
    31921 ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat caaacagcgg
    31981 atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccgccgt tttcgcccgg
    32041 cgtcccctgg cctttcaggc ggatgcgttg accattgccg acgcctgccg ggatcttcac
    32101 attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt
    32161 atagctgatg gtacgcttat gctcagtaag cgtttcttcg aggaataccg ccacttcgat
    32221 ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc tggcgggcat gctgaccgaa
    32281 aattgacgag aagatatcgt caaaatcttc ggcgttaaaa ctctgaccgt cgccatggtg
    32341 gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg
    32401 acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc
    32461 atccggttct ttgctgacat caggatggta tttgcgggca agtcgacgat aggcggtctt
    32521 gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc
    32581 catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta
    32641 taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat
    32701 cgcggagagg aggtcgcctc aaggtggggt gctgaacata ttttaaacag gtgaaaaagg
    32761 gtgagcgatt tttgatagtt gaaacaggca ctttaagttt tactagtgcg tcattattta
    32821 ttaaatttta taggcgctat atatgggtaa tatacatgga attagttgca ctgcaaataa
    32881 ttatttgaag caagcctgga acgatataaa aaatgagtac gaaaaaaata aaacatattc
    32941 aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttgtataaag aaatcagacg
    33001 tcaacaagct gaagactaca ttccatgcct ggaatgcgat tcactagaaa aagaatttga
    33061 ggaaatacag aatgataatg atttatcatt atttatgaaa acattgcgta ctaatgataa
    33121 acaaatttat tcaggggttt caggaggtat tacatatact atacaatatg ttgaagatgt
    33181 tgatattgtt agagtgtcct taccgggcag agggtcagag tctatcacag attttaaagg
    33241 ttattattgg tatggcttta tggaatatat tgaaaacatt aatgcgtgtg atgatgtttt
    33301 ttctgagtat tgtttagatg atgaaaatat tagtcttcag ccagaatgga taaatgcgcc
    33361 gggaatatct gatttagata ctggtattga tttatctggt atatctttta ttcagcgtga
    33421 aactaaccag gcattaggat taaaatatgc tcctgtagat ggcgatggct attgtctgtt
    33481 aagggccata ctggttttaa aagaacatga gtattcatgg gcgctgggca gccataagac
    33541 gcaaaagcaa gtatacgaag agtttattaa aattgttgat aaacaaacga tcgaggctct
    33601 tgttgatacg gcattctata atctcaggga agatgtaaag acgttatttg gcgttgatct
    33661 acaatctgac aacaaaattc agggggaggg tagtcttatg tcatggaact ttctgttttt
    33721 taaaaaagaa tttattgata gttgcttgaa tgacaaaaaa tgtatactgc atttacccga
    33781 gtttatattt aatgataaca aggcacgcct tgttttagac accgacccag agaagaggat
    33841 taatgaggtg aaaaatttcc tcactgctct ttcagatagc atttgctcat tatttattgt
    33901 taatagtaat gtggcatcaa tctccttggg gaatgaatcc ttttcaacag atgaagatct
    33961 tgagtatggt tatttaataa acaccggcaa tcattatgac gtttacctcc ctcctgaact
    34021 ttttgctcag gcttacaagt taaacaataa ggaaatgaat gcgcaacttg actatttaaa
    34081 tcgttatgca atttaatggc aaaggcatat gctaaaaacc attgttatta gtctcacact
    34141 tttttattgt taaatattgt ctctgtattg gtaacgccgc agatattctg tttagccaca
    34201 ggtgcaatta tcagcggcgt acgcgaggca ggggctaatc aggcatagtt tgcgtcaaac
    34261 cttgcctgtt tttgaagatg tatatagaaa aacaggcgtt caacaagcca ttttgcgaac
    34321 ctgttcccgg aaaaaagtca tatttctgtc acactcttta gtgattgata acaaaagagg
    34381 tgccaggaat gaacaaaacg ctaatcaccg caactgtggc agggatagtt ttactcgctt
    34441 caaatgctca ggcacaaacc gtaccggaag gctatcagct acagcaagtg ctcatgatga
    34501 gccgccataa cttacgtgcg ccgctggcga acaatggcag tgtgctggag cagtcgacgc
    34561 cgaataaatg gccagaatgg gacgtccccg gtgggcaact caccaccaaa ggtggcgtgc
    34621 tcgaagtgta tatgggccat tacatgcgtg aatggctggc agagcagggg atggtgaaat
    34681 cgggggaatg cccgcctccg gacaccgttt atgcctatgc caatagtctg caacgtactg
    34741 tcgcgaccgc acagttcttt attaccggcg cattcccggg gtgtgatatt cctgtgcatc
    34801 accaggaaaa aatgggcacc atggacccaa cctttaaccc ggtgatcacc gatgattccg
    34861 ccgcattcag tgaacaggcg gtggcggcaa tggagaaaga gctcagcaaa ctccagctta
    34921 ccgacagcta ccagctactg gaaaaaatcg ttaactataa agattcccct gcctgtaaag
    34981 agaaacagca gtgttcgctg gtggatggca aaaatacctt tagcgcgaag tatcaacaag
    35041 aaccaggtgt ttccgggccg ctgaaagtcg gcaactcgct ggtagatgcg tttactttgc
    35101 aatactacga aggttttccg atggatcagg tggcctgggg agaaatcaaa tctgaccagc
    35161 agtggaaggt gttgtcgaag ctgaaaaacg gttaccagga cagcctgttt acctcaccgg
    35221 aagtggcgcg caatgttgcg aaaccgctgg tcagttatat cgacaaagcc ctggtcaccg
    35281 atcgcaccag tgcaccaaaa atcacggtgt tagttgggca tgactccaac attgcctctc
    35341 tgttaacggc gctggatttc aaaccgtatc agttgcatga ccagaacgaa cgcacgccga
    35401 ttggcggcaa aatcgttttc cagcgttggc atgacagcaa agccaatcgc gatttgatga
    35461 aaatcgagta tgtgtaccag agtgcggaac agttacgtaa tgccgatgcg ttaaccctgc
    35521 aggcacctgc gcagcgtgtg acgctggaat taagcggttg cccgatagat gccaacggtt
    35581 tctgcccgat ggataagttt gatagcgtgt tgaatgaagc ggtgaaataa cagaaaactc
    35641 ccccgtgaga aacgggggag tcgctggtta aacgttttta cgttctatgg tctgttcgcc
    35701 ccaaaaaagc gaatctttat cggtcttagc aaaggctttg actaacactt catcactacc
    35761 ttcttcccaa atcttttccg ccattttttc gtcgtacccg gcgacttcga aaatggcctc
    35821 agctatttcc ggcgacgtat tgcgcagaga tgcccattca ccgacgtgat gagctttcgc
    35881 ttcttgagtt ggcatgcgta tcctcctgtt gaagattagc cattaagttt aactgccaga
    35941 cctgcgacat attccccttg ataacgagca atagacagtt cttcctggct gggctggcgt
    36001 gaaccgtcac cgcctgcgat ggtggttgcg ccgtacggcg taccgccgcg aacctgtgaa
    36061 acgtcaaata attcctgcgc tgcgtagcca atggggacaa tcaccatgcc gtgatgcgca
    36121 agggtcgtcc aggtggatgt gatggtttgt tcctgaccgc cgccagtacc ggtggaacta
    36181 aagacgctcg ccagttttcc gtatagtgcg ccggaagccc acaggccgcc cgtctggtcg
    36241 aggaaggtac gcatttgacc ggacatgttg ccaaagcggg taggtgtacc aaaaataatg
    36301 gcgtcgtaat cggccagttc ttgcggggtt gcaaccggtg cagtttgcgt tttaccgcct
    36361 gctttttcaa ataattgcgg cggcatggtt tccggtacac gcttaacgac aacttcagcg
    36421 ccatccactt tgcttgcacc ctcagcgact gcgcgtgcca tcgtttcaat atgtccgtac
    36481 atggaataat aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat
    36541 tccgtagcga ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt
    36601 cacaaccact tatttgattt ataacaactt tcacaagcac gcaattttgt cgcaaaatga
    36661 cacattttta tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga
    36721 gatgatatct attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtagaaaca
    36781 cgtcagttca ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt
    36841 taattctatg caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag
    36901 gcggttccgg caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg
    36961 gacagcacag cgggggtaat ttcaagaatg atccgcagcg cgcatctgaa gcaggtaaaa
    37021 aaggtggcaa gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg
    37081 catttttttc ttaagctcgt caaaaagtca tcaccgccgg taatcccggc ggttttctgt
    37141 ttatggctcc tgatgaacaa cttctggcgg tggaacgtca accaaccttc tgcttaataa
    37201 cgcattgagt aaaatcgcgc caaaggttgc tgtaccgatc cctcccaacg taaaaccgcc
    37261 cagcgtgaga gcaaaatcac ccgcgcccag cactaaggtt actgcgacca taatcaaatt
    37321 accgttctgg cttaaatcga cacggttttg tacccatatc cttgcgcctg cgacggcaat
    37381 cagcccgaac acaacaattg atgcaccacc aatcaccgcg gccggaatgg tatgaatcag
    37441 cgcgccaaat ttcggtgaaa agcccaatag catggcgatg acggcagcag cgacaaacac
    37501 cagcgtcgag tagacttttg tcacggccat taccccgata ttctcggcat aggtggtgac
    37561 gccgctgcca ccgacagaac cagaaagcat cgtcgccagc ccatcaccga cgaatgcccg
    37621 tcccatatac ggatccatat tgcgtccggt catcccggcg accgccttga gatggcctaa
    37681 gttttccgcc accagaatca ccgccacggg cgcaatcagc atcattgcct gaccattaaa
    37741 accaggagtg gaaaaatgcg gtagaccgaa ccaggcagca tggctgacga gagtaaaatc
    37801 gacggctttt cccagcccta aaacgttggt catcacgcca tacagcagac aggcgacaat
    37861 taatcctaca agaatcaata accgctggat catgccacgg gtaaacaccg ccaccagccc
    37921 aatacacagc accgtcatta ccgccatcca gctatcaaag cctgaagccg atacactttt
    37981 taccgcgata ggcgctaagt tcaggccaat cgccatcacc accgcacccg tcaccaccgg
    38041 cggcattagc cgttcaatcc agcgcgtacc gattttcatc accaccaggc caatgacggt
    38101 ataaaccagc ccacaggcga taatcccgcc cagcgcgatg ctgatattcg ggttaatccc
    38161 ctggccgtta aagcccgttg ccgcgatcac cacaccaaca aaagccgcgc tggagccgag
    38221 ataactgggc acgcgcccgc cggtaataaa gaaaaacagt aacgtgccga tccctgacat
    38281 taaaatggaa agattgggat ccagccccat cagaatcggc attaacaccg tcgcgccaaa
    38341 catcgccacc gcgtgttgaa cgcccattat tgccgtctga gcaaacggca atcgttcatc
    38401 cggcgcgacc acgccgctct ctgtagaggt cgattttaac tgccagtgag gaagaccgaa
    38461 cattgccatc agctgtctcc ttaaggaggt taacaagcag ggcgcatcag cgcgtgataa
    38521 ctgcgatcga accacaccag cccgtagggt gtggtgtgac gatgaatcgc ttcgatggcg
    38581 caaaacaaaa tgtcgtgggt gccgacgctc accacctggc tgatacggca gtcaaacgaa
    38641 accagcgcct cttccagttg cgggcatccg gtcacaccag tctgccagcg ggcggcggca
    38701 aagcggtgtt ccatgggcgt tttgccgcca aaaaggtttg aaagcggctc ctgcccggcg
    38761 ctaagtgtat ttacacacag cgttcgattt tcattgaata ccggccagac ggacgcccca
    38821 cgattcaggc acaccagtaa tgtgggaggc gtatcggtca cgctgcagac ggcgctggcg
    38881 gtgaacccgg cgcgcccggc tggaccgtcc gtggtgatga tattgaccgc cgcacccatg
    38941 caggacatcg catcgcgaaa agtttgttga tcgacaatgt tcatagtttg ctccttacaa
    39001 cagcccgcag gcttcttcaa aggacagacg tggcaggcgc gcaaaaagtt tgctgctatc
    39061 gccatagccg atattaatca gcagattgct cttcagcgtg ctacccgcaa aaaaggcgtc
    39121 gtccacgtgt tgacggtcaa aacccgacat cgggccagta tccagcccca gcgcacggca
    39181 ggcgacgatc agataggccg cctgcatgga actgttgcga aacgctgttt cttcggcaag
    39241 ttgtgggctg gaggtaaacc aactgcgggc atcgccgtga ggaaacagtt gcggtaaccg
    39301 ttcataaaat tcactgtccc atgcgacgat agcggtgacg ggcgcggtca gggttttttg
    39361 caaattgccg ctggaaagcg ccgggcgcag acgttctttt ccctctgcag tgcggataaa
    39421 cacgatacgt gccggagaac agttagctga tgtcggcccc catttcatca gggcataaag
    39481 ctcccgtaac gtctcatcgc tgacgggtgt ctcccgccag ccattgtgag tgcgggcatt
    39541 ggtgaacagg gtgctaagcg cacctgggct aacggcttcg ttcatagcaa ttccttacag
    39601 ggcggcttca cggtgatgta acaggctggc aagcccgttg agtaacagag cattaaacgt
    39661 ttcgggatcg gtcacgttgc aggcgtgtcc gccatagcgc atcaccattt tctggctatc
    39721 gggcagggcg gcatgaagtt cactggaaca tgctgatggc accagcagat catcactggc
    39781 gcagatgatt tgcaccgggc agcggatgcg atccgcatgg tgactaaagt cagcgcgttt
    39841 gagggcgtta agtcgacgca gtaaattatt tttgccctga aaatgcgcca gtgccagcgc
    39901 gtcttctgcc tccaggcgag gtgcgcgggc cgccatccag tcggcgggat agaggaacaa
    39961 cggctgcgct tccacccatg cctgcgcgcc gccgctatac agtaatcgtt cgcgaacctg
    40021 aaaacagcgg cgcgtatggg cgtttattcg tagccagccg ttaacgctga ccagcacagt
    40081 taccgacgcg ggataatcca gcgccagctg cattcccacc agcgcaccga gcgcatggcc
    40141 gaccaccgcg taatgctcaa tccctgcggc taccagcgcc tgatgcagtt ccgctgccat
    40201 ctgggcgata ctgtaatctt ctgccagtgt gtcgggatta ttgccggtgc cgcgctggtc
    40261 gtaacagact acctgatact cctgctccag caccgccagt tgcggtaacc agtaactgcc
    40321 gctaccgcca agacccgaaa tcaacaccac tacgggtgca tcagcataag ggggaggtga
    40381 gagtgaaagt ttcatcgcgg cctcacttgg cgatatgcgc aattgtggcg atttccacca
    40441 gcgcgtcagg ttttaccagt ccgcactgaa tgcagaatcg cgccggttta tcacctggaa
    40501 aaaactcggc gtagatttcg ttaatcgcgg cgtaattttt ccagtcggta ataaagatgc
    40561 tgttgaaggt cacatccgcc atcgtgccac ccgctgtctc gatcaccttg cggatagttt
    40621 ccagaacgtg gcgggtttgc gcctttgggt catcggcaaa cagcacgtta ttgtgttgat
    40681 caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg ggaacgaagg
    40741 gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc gttaaactcc
    40801 ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt cgacgtcgct
    40861 gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtacaa attccggccc
    40921 cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa agccgtcgcg
    40981 tagcgtcgat tcgacgcaga cgttggtagc gatgccggtg aaaaccagat ggcgtattcc
    41041 gcggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc gcggcttcgg
    41101 cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat cccaggagcc
    41161 tttcgccagc aatttcccct gtagctgcgg ctgcttacgc atggttttca aggcgttcga
    41221 tttatgaaaa ttcggtgagc cggggccgcc agcctcgaca tactgttcat cccagccatt
    41281 ttgaaaccag atgatcaaca tccctgccgc tcgcgctgcg gtcacggcgg tttgaatgtt
    41341 ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt agccgcctgg
    41401 cgtggcatag gcgttttgca tatccaccac gatcagcgca ctttgctgcg gatcgaaggt
    41461 aatggcttcc ggtcgagcgg ttaatgtcgt catcatgcca cctcctgagt cagcacaggg
    41521 agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc gattcccgac
    41581 agaaaatcgt cgaaggttaa cagcacgcct tcggcaccag gcacgcttgc gacttcatct
    41641 aacatgcgcg cgacactggc gtaagaaccg actaacgtcc ccatattgat gtttaccgcc
    41701 gaagtgggat ctgccatctg acggacgttg gtgtcagtac cggagcgggt atctttctga
    41761 ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta gtgttcccat
    41821 ttggcacgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac ataagagcca
    41881 acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg ggcgaaagcc
    41941 gtaggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg agcggagaac
    42001 gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact cggttgcgga
    42061 ctgacgcgac aatcattcat ggtgaaaaaa tagcctttaa aatcgctttt tcccgtgccc
    42121 cacaggtcgc gcaacacctg aacatattcg gtgagatagt cgtaacgacg ggagaaatag
    42181 tcatcgccag gccagatacc catctgctca tactcgggct tttgccagcc agtcacgagg
    42241 ttgacgccaa aacgcccgcc agagatggag tcgatggttg cggccatacg ggcgacgatt
    42301 gccggaggta acgttaaggt ggcggcggtg gcgtaaatct gaatgcgcga ggtcacggct
    42361 gccagccccg ccatcaaggt gaacgactca aggttgtgat cccagaactc agttttgccg
    42421 ccaaagccac gcagtttgat catcgacagg gcgaaatcaa aatggtagtg ctccgctttt
    42481 tgcacgatgg ctttattcag ttcaaaggtc ggcatgtact gcggcgcgtg ggtcgaaatg
    42541 agccagccgt tgttgccaat aggtacgaat acgccaattt tcatcatcaa cctctcttcg
    42601 tctcgtaaag tgaaagtcag acggggcgct gcatcctgca tatccttttc agccgcgtat
    42661 tggcttgttt gcaaagtgga tgccagtttt taaaaagt
//
LOCUS       DAZGCQ010000036        41100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000036 DAZGCQ010000000
VERSION     DAZGCQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.036"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..917
                     /gene="espX2"
                     /locus_tag="ACHK07_002061"
     CDS             <1..917
                     /gene="espX2"
                     /locus_tag="ACHK07_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO8102391.1"
                     /translation="KNLAFDLYHNYFALPEIKQFVTEDIKLNFNEQQGLLDKDNKCYI
                     LLSSDNSGRVMRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPETICTR
                     AFPLFAKGWEYAQRNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVPECNNQPTELNS
                     SFSSLMNDSDQWQQHTLKDKHYASLLTMLDLNEASESDKSKIFFCLSAVFANISHSNV
                     FNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQLRI
                     SSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1201..3351
                     /gene="dinG"
                     /locus_tag="ACHK07_002062"
     CDS             1201..3351
                     /gene="dinG"
                     /locus_tag="ACHK07_002062"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO8102392.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3379..4341
                     /gene="ybiB"
                     /locus_tag="ACHK07_002063"
     CDS             3379..4341
                     /gene="ybiB"
                     /locus_tag="ACHK07_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO8102393.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4482..5567
                     /gene="hcxB"
                     /locus_tag="ACHK07_002064"
     CDS             4482..5567
                     /gene="hcxB"
                     /locus_tag="ACHK07_002064"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO8102394.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            5634..5693
                     /gene="ybiE"
                     /locus_tag="ACHK07_002065"
     CDS             5634..5693
                     /gene="ybiE"
                     /locus_tag="ACHK07_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO8102395.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5796..6056)
                     /gene="ybiJ"
                     /locus_tag="ACHK07_002066"
     CDS             complement(5796..6056)
                     /gene="ybiJ"
                     /locus_tag="ACHK07_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO8102396.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6321..6587)
                     /gene="ybiI"
                     /locus_tag="ACHK07_002067"
     CDS             complement(6321..6587)
                     /gene="ybiI"
                     /locus_tag="ACHK07_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO8102397.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(6661..7338)
                     /gene="ybiX"
                     /locus_tag="ACHK07_002068"
     CDS             complement(6661..7338)
                     /gene="ybiX"
                     /locus_tag="ACHK07_002068"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO8102398.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(7380..9662)
                     /gene="fiu"
                     /locus_tag="ACHK07_002069"
     CDS             complement(7380..9662)
                     /gene="fiu"
                     /locus_tag="ACHK07_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO8102399.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9927..10187)
                     /gene="mcbA"
                     /locus_tag="ACHK07_002070"
     CDS             complement(9927..10187)
                     /gene="mcbA"
                     /locus_tag="ACHK07_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO8102400.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10463..11389
                     /gene="rlmF"
                     /locus_tag="ACHK07_002071"
     CDS             10463..11389
                     /gene="rlmF"
                     /locus_tag="ACHK07_002071"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO8102401.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11386..13611)
                     /gene="ybiO"
                     /locus_tag="ACHK07_002072"
     CDS             complement(11386..13611)
                     /gene="ybiO"
                     /locus_tag="ACHK07_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO8102402.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13872..14594)
                     /gene="glnQ"
                     /locus_tag="ACHK07_002073"
     CDS             complement(13872..14594)
                     /gene="glnQ"
                     /locus_tag="ACHK07_002073"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO8102403.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14591..15250)
                     /gene="glnP"
                     /locus_tag="ACHK07_002074"
     CDS             complement(14591..15250)
                     /gene="glnP"
                     /locus_tag="ACHK07_002074"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO8102404.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15389..16135)
                     /gene="glnH"
                     /locus_tag="ACHK07_002075"
     CDS             complement(15389..16135)
                     /gene="glnH"
                     /locus_tag="ACHK07_002075"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO8102405.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16539..17042)
                     /gene="dps"
                     /locus_tag="ACHK07_002076"
                     /gene_synonym="pexB"
     CDS             complement(16539..17042)
                     /gene="dps"
                     /locus_tag="ACHK07_002076"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO8102406.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17341..18228)
                     /gene="rhtA"
                     /locus_tag="ACHK07_002077"
     CDS             complement(17341..18228)
                     /gene="rhtA"
                     /locus_tag="ACHK07_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO8102407.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18463..18528
                     /locus_tag="ACHK07_002078"
     CDS             18463..18528
                     /locus_tag="ACHK07_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102408.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18581..19096
                     /gene="ompX"
                     /locus_tag="ACHK07_002079"
     CDS             18581..19096
                     /gene="ompX"
                     /locus_tag="ACHK07_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO8102409.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(19146..20729)
                     /gene="opgE"
                     /locus_tag="ACHK07_002080"
     CDS             complement(19146..20729)
                     /gene="opgE"
                     /locus_tag="ACHK07_002080"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8102410.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKDGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(21001..21129)
                     /gene="mntS"
                     /locus_tag="ACHK07_002081"
     CDS             complement(21001..21129)
                     /gene="mntS"
                     /locus_tag="ACHK07_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO8102411.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21315..21782
                     /gene="mntR"
                     /locus_tag="ACHK07_002082"
     CDS             21315..21782
                     /gene="mntR"
                     /locus_tag="ACHK07_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO8102412.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21779..22897
                     /gene="ybiR"
                     /locus_tag="ACHK07_002083"
     CDS             21779..22897
                     /gene="ybiR"
                     /locus_tag="ACHK07_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO8102413.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22955..23875)
                     /gene="ldtB"
                     /locus_tag="ACHK07_002084"
     CDS             complement(22955..23875)
                     /gene="ldtB"
                     /locus_tag="ACHK07_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8102414.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            24094..25686
                     /gene="ybiT"
                     /locus_tag="ACHK07_002085"
     CDS             24094..25686
                     /gene="ybiT"
                     /locus_tag="ACHK07_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO8102415.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25927..27192)
                     /gene="ybiU"
                     /locus_tag="ACHK07_002086"
     CDS             complement(25927..27192)
                     /gene="ybiU"
                     /locus_tag="ACHK07_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO8102416.1"
                     /translation="MAPTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAG
                     DSVWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(27339..28154)
                     /gene="ybiV"
                     /locus_tag="ACHK07_002087"
     CDS             complement(27339..28154)
                     /gene="ybiV"
                     /locus_tag="ACHK07_002087"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO8102417.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(28300..30732)
                     /gene="ybiW"
                     /locus_tag="ACHK07_002088"
     CDS             complement(28300..30732)
                     /gene="ybiW"
                     /locus_tag="ACHK07_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO8102418.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRFHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(30738..31637)
                     /gene="ybiY"
                     /locus_tag="ACHK07_002089"
     CDS             complement(30738..31637)
                     /gene="ybiY"
                     /locus_tag="ACHK07_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO8102419.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31768..32430
                     /gene="fsa"
                     /locus_tag="ACHK07_002090"
     CDS             31768..32430
                     /gene="fsa"
                     /locus_tag="ACHK07_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8102420.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32506..33255)
                     /gene="moeB"
                     /locus_tag="ACHK07_002091"
     CDS             complement(32506..33255)
                     /gene="moeB"
                     /locus_tag="ACHK07_002091"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO8102421.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(33255..34490)
                     /gene="moeA"
                     /locus_tag="ACHK07_002092"
     CDS             complement(33255..34490)
                     /gene="moeA"
                     /locus_tag="ACHK07_002092"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO8102422.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            34694..35659
                     /gene="iaaA"
                     /locus_tag="ACHK07_002093"
     CDS             34694..35659
                     /gene="iaaA"
                     /locus_tag="ACHK07_002093"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8102423.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSSHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            35646..37517
                     /gene="gsiA"
                     /locus_tag="ACHK07_002094"
     CDS             35646..37517
                     /gene="gsiA"
                     /locus_tag="ACHK07_002094"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO8102424.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQVLRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAPVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37537..39075
                     /gene="gsiB"
                     /locus_tag="ACHK07_002095"
     CDS             37537..39075
                     /gene="gsiB"
                     /locus_tag="ACHK07_002095"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO8102425.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            39093..40013
                     /gene="gsiC"
                     /locus_tag="ACHK07_002096"
     CDS             39093..40013
                     /gene="gsiC"
                     /locus_tag="ACHK07_002096"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO8102426.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            40016..40927
                     /gene="gsiD"
                     /locus_tag="ACHK07_002097"
     CDS             40016..40927
                     /gene="gsiD"
                     /locus_tag="ACHK07_002097"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO8102427.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIMVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         9839 a        10187 c        11047 g        10027 t
ORIGIN      
        1 agaaaaactt agcatttgac ctttatcaca attattttgc attacctgaa ataaaacaat
       61 tcgttactga agacataaaa ttgaatttca atgagcagca aggattgtta gataaagata
      121 ataaatgtta tatcctgtta tcatccgata actctgggcg agtaatgcgc ttatcgcacc
      181 aggcgttaat ctcaatgctt gagccagagg ttaagaaaaa gactatctgg aataattact
      241 ctatttatcc atctttgcag gatacccatg aagttgtcag agatgatcca gaaacgattt
      301 gtacgcgtgc atttccttta tttgcaaaag gatgggaata tgcgcagaga aataagaaac
      361 accaattgat tctcaatgct cttggattca aaggttatat tcgtgatata tttatgtcag
      421 caataatgcg aaaaacagat tttgtgcccg aatgcaataa tcaaccaaca gaacttaatt
      481 catcattttc ctctttaatg aacgattcag atcaatggca acagcatact ctgaaagata
      541 aacattacgc tagtctatta acgatgctgg atctcaacga ggccagtgaa agtgataagt
      601 ctaaaatatt tttctgcctg tctgccgtat ttgcaaatat ttctcacagt aatgtcttta
      661 acggtattcc tgatgcgtca aaaactttaa aacgttatgc atttgcttta ttggcgaaag
      721 ctcattcttt agatgaaagt atgataagca atcaaacttt taatacatac aaaacggtat
      781 tgcttgattt caataatctg tctaatgaag aggccaacca gttgcgcata agttcgttat
      841 atcgtgatat ggtccgctat gcacaatatc gtttttctaa agttttatcc gagtggacgc
      901 cagacgcatg gctgtaaaga tcgcagcaga ataatggcat tgatggccct ggctcaggcc
      961 agggtcggca ataccggttc ctgcgaagcg atgacggcgg caagtgtaac cacgaaatgc
     1021 ggtatcactt gctgttttct gccctgaaga ctggcgctca ctgtgccgtc gaattttcaa
     1081 tcaaatatat tgcgagaggg cgtgtttgcg agccgctttc cagaaacaga aaaaccatta
     1141 cccctgaaaa ccgaaaaatg ccacaatatt ggctgtttat acagtatttc aggttttctc
     1201 atggcattaa ccgccgcgct taaagcgcaa attgccgcct ggtataaggc gcttcaggaa
     1261 cagatccccg actttattcc ccgtgcgccg cagcggcaga tgatcgcgga cgtcgccaaa
     1321 acgctggccg gagaagaagg gcggcatctg gcgattgaag cccccactgg cgttgggaaa
     1381 acgctctcct atttgattcc cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg
     1441 gtgagcaccg ccaacgtggc attgcaggat cagatctaca gcaaagattt accgttgctg
     1501 aaaaagatca ttcccgatct taaattcact gccgcttttg ggcgtgggcg ctacgtttgc
     1561 ccgcgtaatc tgacggcgct tgccagcact gagcccacgc aacaggatct gctggcgttt
     1621 cttgacgacg aactgacgcc aaataatcag gaagagcaaa aacgttgtgc gaagctgaag
     1681 ggcgatctcg acacttataa atgggatggt ctgcgcgatc atacggatat cgctatagat
     1741 gacgatctct ggcgtcgttt aagtaccgac aaagccagct gcctcaaccg caactgttac
     1801 tactatcgcg aatgcccgtt ttttgttgct cgtcgggaga ttcaggaagc ggaagtggtg
     1861 gtggcaaacc atgcgctggt gatggcggcg atggaaagcg aagcggtatt gcctgacccg
     1921 aaaaatttac tgctggtgct ggacgaaggc catcacctgc cagatgtggc gcgggatgcg
     1981 ctggagatga gtgccgaaat caccgcgcca tggtatcggc tacagctgga cttgttcacg
     2041 aaactggtcg ctacctgcat ggagcagttt cgcccgaaga ccatcccgcc gctggcgatc
     2101 cctgaacgtt tgaatgcgca ttgtgaagag ctgtatgagc ttatagcctc attaaacaac
     2161 attctcaatc tctacatgcc tgctgggcag gaagcagaac accgttttgc gatgggcgaa
     2221 ctgcctgatg aagtgctgga gatctgccag cggctggcaa aactcaccga gatgctgcgt
     2281 ggtctggcgg agttatttct taacgattta agtgagaaaa ccggcagcca tgacattgta
     2341 cgtctgcatc ggttgatttt gcagatgaac cgcgcgttgg ggatgttcga ggcgcaaagc
     2401 aaactctggc ggctggcttc gctggcgcaa tcttccggtg caccggtgac caaatgggcg
     2461 acgcgggaag agcgcgaagg gcagctacac ctctggtttc actgcgtggg aatacgtgtc
     2521 agcgatcagc tggaaaggct gctgtggcgc agtattccgc acattattgt cacctccgca
     2581 accttgcgtt cgctgaacag tttttcgcgt ttgcaggaga tgagcgggct gaaagagaaa
     2641 gcgggcgacc gttttgtggc gctggattcc ccctttaacc actgcgaaca gggcaaaatt
     2701 gttattcccc ggatgcgcgt tgagccttcc atcgacaacg aagaacagca tattgctgaa
     2761 atggcggcct ttttccgtga gcaggtggag agcaaaaaac atctcggtat gttggtgctg
     2821 tttgccagcg ggcgtgcgat gcagcgcttt ctcgactatg tgacggattt acgtctgatg
     2881 ttgctggtgc agggcgatca gccgcgttac cgtttagttg aactgcaccg caaacgcgtc
     2941 gccaacggtg agcgtagtgt gctggtgggc ttacagtcat ttgccgaagg gcttgatttg
     3001 aaaggtgata tgctcagcca ggtgcatatc cacaaaatcg cttttccgcc tatcgacagc
     3061 ccggtggtga tcaccgaagg ggaatggctg aaaagcctca accgctatcc atttgaggtg
     3121 caaagcttgc cgagcgcctc gtttaacctg attcagcagg ttgggcgact gattcgaagt
     3181 cacggttgct ggggcgaagt ggttatctac gacaaacgct tgctgaccaa aaattatggc
     3241 aagcggctac tggatgcatt accggtattt ccgatagagc aaccggaagt ccctgaaggt
     3301 atagttaaaa agaaagaaaa aacgaaatcc ccacgccgtc ggcggcgtta atgatgtaag
     3361 tcaggtaagg agtcgtaaat ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa
     3421 aaccacgcgc gcgatttaga ccgggatact gcgcgcggtc tgtatgccca tatgctcaac
     3481 ggtgaagtcc ctgaccttga gttgggcggt gtattgattg cgctgcgtat caaaggggaa
     3541 ggggaagcag agatgctcgg cttttacgaa gccatgcaaa atcacaccat caagctgacg
     3601 ccgcctgcag gcaagccgat gccgattgtc atccccagtt acaacggcgc gcgtaaacag
     3661 gccaacctga cgccgttgtt ggcgattctc ctgcacaaac tcggttttcc tgtggtggtt
     3721 cacggggtta gcgaagatcc aactcgcgtg ctgaccgaaa ccattttcga actgatgggt
     3781 attacgccaa cgttgcacgg cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg
     3841 tttatgcctg tcggcgcgtt ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg
     3901 atgggcgtgc gtaacagtgc acataccctg gcgaaactgg cgacgccatt tgccgaaggt
     3961 gaggcactgc gtctttccag cgtttcgcat ccggaataca tcgggcgagt cgcgaagttc
     4021 tttagcgata tcggcgggcg ggcgctacta atgcatggta ctgaaggtga agtgtatgct
     4081 aatccgcagc gctgcccgca gatcaatctc attgaccgtg aagggatgcg ggtgctgtat
     4141 gaaaaacagg accctgctgg tagcgagtta ttgccacaag caaaagatcc ggaaaccacg
     4201 gcgcagtgga ttgagcgttg cctggctggc agcgaaccga ttcccgaatc gctgaaaatc
     4261 cagatggctt gctgcctggt ggctacgggt gaagcggcaa ctatcagcga tggcctggcg
     4321 cgcgttaatc aggcatttta atttttatct cagccggatg agccacgtca tccggctttt
     4381 accccaccgc aatatgaaat tcctgcatct ttattgacct tctcgcgccc ggcgtgcagc
     4441 ataaaaaaac aacaaacaca taacataaac aggagttaac catggaaagt ggtcatcgct
     4501 ttgatgctca gacgcttcac agctttattc aggctgtatt tcgtcagatg ggtagcgagg
     4561 aacaagaagc gaaattagtt gccgatcatt taatcgcggc aaacctggca gggcatgatt
     4621 cacatggtat tggcatgatc ccaagctatg tacgctcctg gagtcagggg cacctgcaaa
     4681 ttaaccatca tgccaaaatc gttaaagagg cgggggcggc ggtcacgctc gatggcgatc
     4741 gcgcatttgg tcaggtcgcg gcacatgaag cgatggcgct ggggattgag aaagcgcatc
     4801 agcacggtat tgccgccgtg gcgctacata actcgcatca tatcggccgt atcggttact
     4861 gggcggagca gtgtgcagcg gcggggtttg tctctatcca ctttgttagc gtggtcggta
     4921 ttccaatggt cgcgccgttc cacggtcgcg acagccgctt tggcaccaat ccgttctgtg
     4981 tggttttccc acgtaaagat gattttccgc tgttgcttga ttacgccacc agcgccattg
     5041 catttggcaa aactcgcgtc gcctggcata aaggcgtccc cgtgccgcca ggttgtctga
     5101 ttgacgttaa cggcgtgccg acgacaaatc cggcggtaat gcaggagtcg ccgttgggtt
     5161 cgctgttgac ctttgccgaa cataaaggct acgcccttgc agcgatgtgt gaaattcttg
     5221 gcggggcgct ttctggcggt aaaacgacgc atcaggaaac gttacaaacc agtcccgatg
     5281 ccattcttaa ctgcatgacc actatcatca tcaatccgga actgttcggc gcgccggatt
     5341 gtagcgcgca gaccgaagcc tttgccgagt gggtgaaagc ctcgccgcat gatgacgata
     5401 agccgatttt gctcccgggc gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg
     5461 ggattccatt ggatggggga agctggcagg ccatttgtga tgcggcgcgg cagattggta
     5521 tgtcggaaga gacgttgcag gctttctgtc agcagttagc cagctaaaaa aaagcccgtc
     5581 cagtggcgga cgggcaaaca agggtaacat aggatcaatg agggttagag catatgcgtc
     5641 tgtcggcaaa cagacaggga aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc
     5701 aacggttcgg attttgtagg cctgataaga cgcggtagcg tcgcatcagg catctgttgc
     5761 cggatgcggc gtaaacgcct tatccgtcct acgaattact tataaattac cgcagtaccg
     5821 cttaatttat tgttgttggt ggcggaagtg atgctgtatc cactcgcccc agctgccgcc
     5881 gctttctcag ccagtttcgc ttccagggca tcgagggtgg atgcgccatc ggcagaaacc
     5941 acgccgattt tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc ggcaaacacg
     6001 ccaaatgaca gagttgaaag agccatagca gcaacaacag tattgatagt tttcataatt
     6061 aatctctcgc aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac
     6121 gtttttttac gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg
     6181 atatctaaca tactgataaa ccatttattt gttgtactta tggacttgtt ttatgcagcg
     6241 tggcagtcac agcagcgtta aaggcacgga atgccacgca aaatacaaat tatactcgct
     6301 atacatctcg ccagttgtag ttaacgtaac tggctgtctt tcgaacctct gcgattatat
     6361 cctgtataag ctggtttttg taaatctttc tcctgctgac aatgaataca taagcgcacg
     6421 ccaggaatgg cttcccgacg ggcttgcggg atgggggcac cgcactcttc acattcatac
     6481 aggctttcgc cacgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg
     6541 ttgatctgtt cgttgacggc gtcatcgtta gcccaaccgg atgccatatc gtcctcccca
     6601 tatcaatgct tgtacagtta agtgtagcta atatagggac gaattcgggc agttcaagcg
     6661 tcagatctcc gaccattccc gcagcagatt atgataaaga ttgagcagcg acaggatctc
     6721 ttcactttcg ccgtagcggc ttttcagcga ctgaatattg ttgtccagtt caaacagcat
     6781 ggcgcgcttt ttatcatcac ggatcatcga ctggatccac ataaatgatg ccactcgtac
     6841 gccgcgggtt acgggtgtca cgcaatgcag gctgctggag ggatacaaca ccagatcgcc
     6901 tgccgggagt tttacccgat gttgtccgaa ggtgtcatta acgaccagtt cgccgccgtc
     6961 gtagctttgt ggatcgctta aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt
     7021 ttgtggatgg ctgcgtactg cgccatccac atgaaaacca taggtttcat tgttctgata
     7081 gcgattaaac agcggcgtgg aaagggtacg cggcaaggcc gcggcaaaga acaaagcatg
     7141 ttggttaacc gcgttcagca cctcattttg cagggcggcg tataacgtgc tgcgggtgtc
     7201 gacctgttga ttgttcttaa cttgcgcacc ttgtgcgcca gtggtgacgc gtccatccac
     7261 ccattcggct tgttccagtt gttcgcgaaa acgggcgacg tcctgtggcg ataacacgcc
     7321 gggaatgtgg tacatcatac aagttctcca aaaagtgggg cctgcgcccc acatctgaat
     7381 cagaaatgca tattggctgt gagcaagaag gttcttggct cgcccgggtg ataacggtag
     7441 ccgctcttat tgattgaggc gacgtaatcg gtatcaaaca ggttgtagac gtttagctgg
     7501 aagtcgagat tgcgattaac ccgatacccc agtttggcat cggcgaccca gtaaccttcg
     7561 gtaaacgctg gcgttcccac cgcgccgtct gaacctttat gcatactgcc gatatagcgt
     7621 gcgcccgcac ccacagaaat atcatcggtt gcctgatatt ggctccataa ggtgaaggcg
     7681 tgctccgggg tatacggcag cgatgaggaa ccatcctggg caacatcttt gccgtttttg
     7741 atggttgctt tttgctgggt atagccgcca atcacctgcc acgcgggagt gatattccct
     7801 gccacggata tctcatagcc ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca
     7861 tcattttgct caacttcatt ttcgatatca gtacggaaca gcgcggcggt gagcaacagg
     7921 cgtttatcca gaacctgcca tttggtgcca atctcgctgg tgttggcttt ttgcggttta
     7981 aaatcggtgc ggttggcact gttaccactg ccagactgcg caagggcgaa gttgttgccg
     8041 cccggaggct gctgggaaac ggcatagtta atatagacat tgccgttttc cgtcaggtga
     8101 tacagcgccc cggctttcca gttcaccaga ttgcccgact tggcggtgtc gacggtggtg
     8161 actggagaac cttttgccac accagctggg caggtgatgg caccgcgtcc gctgccgccg
     8221 caggcggtgg cactgtcata ttcagtatga taattatcca gacggatccc gccgttcagc
     8281 tcaaaatcac gggtgatttg cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga
     8341 ccattggcgt ttgcgccgtt gcgcgtcagt ccgccgggat gaatgctgct gtcaggatga
     8401 taaatattta ccgccggtaa cgtcaccgga ttaacgccgt agttagtctg cgtttcacgg
     8461 gtaaattcca cgccggtact gacatcatga ccgatagaac cggtatagaa cgtcgaggtc
     8521 aggttggtct ggttggtgag aattttatta ctcacatctt tggtattcgc cgtgcgtgac
     8581 cacgtccagc tattcacatc gctggtgggc tgggtaatat tcgacgcccc acccataatc
     8641 gccgtcatca ggtaatcctg cttcacgcgc gaccaacggg tagtgttgcg aatggtggtg
     8701 ttatcgttga tgtcgtgctc aaaacgcatg gtagcggtgt cggtggtcga atcgtcgtaa
     8761 tcggaatccg tgccgtaaaa gttatgcgta tcaacttttc cggaatgatt cagggccgcc
     8821 gttcccgcag atggggcaga atagcccggc aaaccgatgg ttggaatgcc gccgtctggc
     8881 gtgttgtgct gggtgacatg cagataatta agatacaaac gattcgctgt accaaggcca
     8941 aaagcgacag aaggggcgac gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg
     9001 tgcgtttttt cgcccatcac attcaggcgc gccgcggtgg tatcaccaat gacctgattg
     9061 acgtccagcg tgccgcggcg gaaccaggcg ctaccaatac tggcggaggc gtcaatgccg
     9121 gaatcattgc gcggctgctt gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg
     9181 tagtcggtgc cggacggccc tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc
     9241 gagacgctgc cgatatcgcg aatgccatca atataaatac tgttagaggt atcagcaccg
     9301 cgcatataaa tggcgtcgcc agtggtggag ttaccgttct cacccgcaaa aaacgcaccc
     9361 acgccgggga cgtttttgag tgcgtcggta aggttggttg cgccctgatc tttaatcact
     9421 tgttcagaaa tcaccgtcat cgtgcgggta gtatccgcta ccggacgcga gaatttcgga
     9481 tctgcagatt gttgtggcgc ataaagcgaa ggcgttgatg cttcgacaac cagcgtgtca
     9541 tccgcgttag tttgcccttc ggcggcgagt gcctgagcca caggcgtgat gccaatacaa
     9601 agaccggcaa agaacgtgag cgaatgaaat tgtctggcag ggaaattgcg attgttttcc
     9661 attttgcagg tgactttttc ttatatgttg aaattttcac tgccacgaga aagcctctct
     9721 gcggatgcga tgccactgtt aatctcgtga tagtgttcgt gcaagtgata ataattttga
     9781 taagcattat cacttggcgc gattttctat cacccattgc gcgaaaaaga aatacattta
     9841 tttacattgc gttcatattt gatgtttttt gattacaaaa caggcgaaaa ctgcatccag
     9901 aggcgaatgc agttacgttg gtggggttat ttgtagatgg ttgcagtacc gaacatctgg
     9961 tcatttccgc ctgcggaatt aatgacataa cctttcgcgc cttgctcgcg cgctttttct
    10021 gccagtttat cctccagatc gcttaggttt gatgcgccag ttgccgaaac ggtaccggcg
    10081 ggccgtagct gaccgctgtc caggttactc atcggttgcg cggcataagc cgtgagagag
    10141 attccgctca ggacggtggc aatcagtagt gtgaggcact ttttcataat gacatcctca
    10201 taaatacaac gggtagtgcc tgataagtgt aggtcgcctg ggcgatgaaa atgacgcaaa
    10261 aaattgacag agatacgctt attttttgaa cgatatattt ttacaaaata agacaaatca
    10321 atgacatgca tgatgctgat ttttatgatg aattgccata aaccatagcc atgttaaggt
    10381 ttttagaaat gataaacgca gccagatatt taccctgtcg cgttatgcgg gtatcttacg
    10441 ccgctgctta aaggagaatg ctatgtccgc ccagaaaccg gggttgcatc cgcgcaaccg
    10501 tcatcacagc cgctacgatc tcgccacgct ttgtcaggtc aatcctgaac tcaggcaatt
    10561 cctcacgctt acacccgcag gggagcaaag cgtagacttt gccaatccgc tggcggtgaa
    10621 ggcgctcaat aaggcgttgc tggcccattt ttacgccgta gcgaactggg atatccccga
    10681 cggttttctc tgcccgccag taccgggccg ggcggattat attcatcacc ttgccgattt
    10741 actggcagaa gcaagcggaa caattccagc caatgccagc attctggata tcggcgttgg
    10801 tgcgaactgt atttatccgc tgattggcgt acatgaatat ggctggcgtt ttaccggtag
    10861 tgaaaccagc agccaggcgt taagcagcgc gcaggcgatc atcagtgcta atccggggct
    10921 taatcgcgcc attcgtctgc gtcggcaaaa agagagtggg gcgattttta acggcatcat
    10981 ccataaaaac gagcaatacg acgcgacctt gtgtaacccg ccattccacg attctgccgc
    11041 tgcggcgcgg gcaggtagtg agcgtaaacg ccgtaacctg gggctgaaca aagacgatgc
    11101 actgaacttt ggcggccagc aacaggagtt gtggtgtgaa ggcggtgaag tcacctttat
    11161 caaaaagatg attgaagaga gcaaaggctt cgcgaagcag gtgatgtggt ttacttcact
    11221 ggtatctcgt ggtgaaaact taccgccgtt gtatcgtgct ctgacggacg tgggcgcggt
    11281 gaaggtggtt aaaaaagaga tggcccaggg gcaaaagcag agtcgcttta ttgcctggac
    11341 ctttatgaac gacgagcagc gccgccgttt tgtcaatcgc cagcgttaaa gcgttggctc
    11401 ccccggcggt aacggttcag ccggggtcgc gcccggagca ggcagcacct gataagtctg
    11461 cactggcgcg cgaacgcccg ccaggtcgaa atgttttttc acctggctgt cgagggcaaa
    11521 gcgtaccgtc cactgtttga gcggcagcgt agtgaacgaa acacgcagtg taaacgcggt
    11581 attgcttaag ccgacaatcc cggcaaaatt cggttcacca ataatcagcc cgcgaatttc
    11641 ttcgttttcc attaattccg ctaccgcatc tttcagtgcc tgattggctt tatcagcatc
    11701 ttcatggcga tcaacatcgt agtttgccac taccgaacca atgccgcgga caaagttggc
    11761 aaaggtggta atcgaagacc acggaatgat gtggtacgcc ccggtatcct ggcgtacgcc
    11821 cacggagcga atcgacatcc gttccacagt gccggtcaac ggcccaatag tcaccaaatc
    11881 tccagtgttc atgccgtttt caaactgaat aaataccccg gtgataatat ctttcaccag
    11941 cgtttgcgaa ccaaacgaga tagccagacc taatgccccg gcacctgcca gcaatggcgc
    12001 gatattgacg ccgatttccg acaacacaat catgatggtg atggtactga taatcaccgc
    12061 cagcgcgtta cgaaacagcg taagcagggt acgcgtgcgg gcgctgggta gcgggcggcc
    12121 atgaatatcc gaagccagcc ggttttcgat caaactggcg agcaccgtcc agccaaccgc
    12181 cgagaagaaa agaatgagtg cgatacggat caggatatct acggttttct gccccgcgcc
    12241 gttttgcagc cagttccaga aatcgaacaa tccccatgcg ctcaacagca gcattaccgc
    12301 cacgcagacg gtcagaatac gcgccgtttt cagcgccgcc gaaagccagc cattcagccg
    12361 tttttgcaac tccggatagt tacgctgagt atgtggcgag agagtgatgg tttttgccag
    12421 ccagcgggaa aacataccgg aaacaaacgc tgcgatacca ataatcgcca ggctgcgcac
    12481 cgttgcaccc atcataaatt tcaggctatt gcccggatcg aacaacgaaa agaaaaacag
    12541 cacgataaaa taggcgcttg ccagccagtg ccacaccagc gcaaaggcgc ggataaacag
    12601 gctgaaaaag gccagcgaat gctccgcaaa gttgagcaaa tgctgggtaa tctctttttt
    12661 attacgaaag atcaggtaca acgcccagac ggtcatgcac agcataatga tgacgttcgc
    12721 cagcgcgcca atttgtacat tcacctgatt agagataatc ggcacggcca caatcaggcc
    12781 ataacctatc aaactgctta accagctcag gcgacgactc cagtaacggg cgctctcgtc
    12841 ctgaatcgtg aacgggcgca gttccgccac gtttgggcaa aaaatcaggc gtagtacggc
    12901 tttgaaaaat tcaatcaggg caaaggcgtt gagaaacaaa ctttgttgga aagcgatggt
    12961 gcgactgcct gcattcaggt tgtcgcttaa tacctggccg acaaataatg tcagggccag
    13021 taacagcagg tcgataataa acgccccgat aatcatcgcc ggaagctgca accagttact
    13081 gcgctcacga tttttttgcc gcgcccactg acccattttg cgatacagcg gcagtgcgca
    13141 caggcgaatc agccagtaaa aaccaaacac taaaaccgct aacattgaaa aatgggtcag
    13201 cgcattgctg aaggtttgcg gattaaacgg cttatgcggg gagccggtga tattgcgata
    13261 aagttgcccg aagcgggcgg aaagggcttc accataatgg cggctgactt cggtgacctt
    13321 ttgcagcacg gtttgctctt caaccagcgt cggcggcacg atcttcggga ccggttcagc
    13381 aggtggcgtc gcggcgacgg tgcgcaactg gtcgatcaac tctttacgcg aggtgtcatt
    13441 atccagcaca tccgccagtg cgccataggc cgcttttttt tgttcgatat ccggttccgg
    13501 ggccggttca gtcgttgagt ccgttgtcgt tgtggttgta acgccgggta tggataccgc
    13561 gtgggcaggt gcgcccagca ggcagaagag gatgaacagg atccaccgca tgactcctcc
    13621 agtgagaaaa cagagcaaaa agataagtat agatgctggg gaaggggcga ttttaaatga
    13681 gaggaatctg gtgtgcctcc ctttcgggtg aaagggagga agggatatta agcaggttgc
    13741 tgacaacgtg cgggcttttt tgccggatgc ggcgtgaacg ccttatccgt cctacaagtt
    13801 catgcaattt taataagttg ccagatatcc tgtaggcctg ataagcgtag cgcatcaggc
    13861 aatgtgtctt attaagagac gtgctgcaaa aattcctgca agcgctggct cggcgggttc
    13921 ttgatcaaca cctgcggatc gccatcttcc gcaatccggc ctttatcaat gaagatcagg
    13981 cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct
    14041 tcttcagcca gatcctgcat aaccttcagc acttcatggc gcagttccgg gtcaagagcg
    14101 gaagtcggtt catcaaacag catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc
    14161 acacgctgct gttgaccacc agaaagttcg gaagggtagt gatgtgcacg ttctgccaga
    14221 ccgactttcg ccagcagctc acgtgccagt ttttccgcct cttctttgtt cgcgccacgc
    14281 acgcgtagcg ggccaaacat aacgttttcc agcgccgtca gatgcgggaa aaggtaaaac
    14341 tgctggaaca ccatacctgc ttcctggcga atcaggcgct cgtcaacttt cggatcgtta
    14401 accttcaggc catcgacaat cagatcgccg gaggtgattt cttccagttt gttgatgcag
    14461 cgcagcaggg tcgatttacc ggaaccggac ggcccgataa tcaccacgac ttcgccctgg
    14521 gcaatgttca aatcgatatt gtgcagcacc tgggttgggc caaagtgctt ggagacgttt
    14581 ttaaattcaa tcacaggatt ttcatccttc tttccagacg acgcagaata aagctcagca
    14641 ccagcgtaat aatcagatag aacaccgcca cggcgctcca gatctcaagg gcgcggaagt
    14701 taccggcaat aatttcttgc ccctgacggg tcagttccgc cacgccgata acaataaaca
    14761 gcgaggtgtc tttaatgctg atgatccact ggttacccag cggcggcagc atacgacgca
    14821 gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc
    14881 ctgcttcacg aaaacctttg tggatagaca gcaccgcacc acgcgtgatt tccgcaatgt
    14941 acgcgccgga gttgatcatg atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt
    15001 cgttaaacgc catcggcagg gcgaaataaa taaacatcac ctggacgacg ataggtgtgc
    15061 cgcggatcac ttcaataaag accagcgcga cgtggttggc tatccaacca ccgaaggtgc
    15121 gtgcaaaacc tgccagcaat ccgattacca gaccgccagc cagaccgagg accgaaatcc
    15181 acagggtcat tttggcacct tcaatcagaa gcggaatggc aggccagatg gcactccagt
    15241 caaactgcat atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat
    15301 ggccccggag cgggtttgcg cccctcaccc taatcctctc cccagagggg agagggaact
    15361 gccagtgcgc tttacaggtg tagcgttatt atttcggttc agtaccgaac cattttttgt
    15421 agatttcgtt gtaagttccg ttctcgcgca gggttttcaa cgcgccgttg actttgtcac
    15481 gcagctcgtc gctacctttc gggaacgcta taccgtattg ctgcgcttcc agagagtcac
    15541 ctaccgcttt gaactgaccg ttaccggcgg ttttgatgaa gtacagaatg tttggcgtat
    15601 cgtgcagaac ggcgtctgcg cggttggtgc ccagttccat ataggcgtta tcgatgttcg
    15661 ggaactgacg cagatcttta gttttgatgt ttgctttcgc gtaatcaacg gagccagtac
    15721 cgctcttcac agcaaccact ttcccgtcga gatctttcac gcttttcaca tcgttattgt
    15781 tagctttcac catcactaac aggccgcttt tgtagtagcc gtcagagaaa tcgatcgctt
    15841 ttttacgctc gtcggtgatg gtaatgcccg ccagcgccag atcgacgttt ttggtttgca
    15901 gtgccggaat gatcccactg aaatccatcg gcttcagttc gtaatccagc ttcagctctt
    15961 tagcgatggc agcccacaga tcaacgtcaa agcccacata tttatcgccc tgtttaaatt
    16021 caaacggaac gaaggcggta tccgtcgcga caactaattt tttatccgcg gcatgagaag
    16081 aaaccgcaaa agccagggtc agtgcagcca gtgaaacttt taatacagac ttcatagcat
    16141 ttcctttttt ctatccacgg gacgatcccc tgcgtgacat tcacatatat gaaaaaatcg
    16201 tgccagtttt gcaactcatt gatttttcga gatgcggcaa aaacgtgatg cacgatttat
    16261 agggcaatac cctgaagatg caccattctg gggcaccaaa ctggtgcgct aaaattgtgc
    16321 actcaacaca gtgattattt agcgtaaaac ctgatgaaaa aacaatcttt atgtaacgat
    16381 tgtgtgatga tgtggataca aaaaatttac ttaatcagct ggggatagca gatggatgca
    16441 ctaaataagt gcgttgaggc gggctgcaaa acaaaacggc ctcctgtcag gaagccgctt
    16501 ttatcgggta ctaaagttct gcaccatcag cgatggattt attcgatgtt agactcgata
    16561 aaccacagga atttatccag gtcgcgagac gcggcggtca ggatatctgc ggtgtcgtca
    16621 tctttcgctt cgccaatcgc tttgcgtacg tcattagcga cgattgcgta acggtcagcc
    16681 agttctttca ggtgatcctg aacgttgtgg atgtccagcg ggtaactttt cagcggggtt
    16741 ttgctgttga taacttgagt ggtccccaga gctacaccgc ccagctgcac tgcacgttct
    16801 gccatggtat ccagatgatc gatcagtgca gtgcggaagc catccagcat ttcatgtacg
    16861 gcaatgaagt tagcgccgcg catgttccag tgcgcttgtt tggtaatcaa agaaagatca
    16921 ataaactgga taacctgacg attcagcaac tctactgttg cttttttctc gctgtcagag
    16981 acatcgttgc gggtataaag cagattggtc gcttttgatt taactaattt agcggtactc
    17041 ataatttcat atcctcttga tgttatgtcc cagtaattaa cgagactaag tatagcaccg
    17101 gctatgtgtt ccgctattct ggctgttcct atcacactaa tagtggtaac aagcgtgaaa
    17161 aacaaaacta ataaagattc aatgagttag atatattgat aagaacaatt cttatttatt
    17221 tgctcgccag gaattttcgg aataaatatg cttttgcata attcgatata taaagacggt
    17281 gtagaggaaa agtagcgaga aattctgcat ggttatgcat aaccatgcag aaatgtaaat
    17341 ttaattaatg tctaattctt ttattttgct ctctttgcgt actgtcagcg tagaacccat
    17401 tgaagcggcg atgatagcgc cgagcgccag cagctgtatg ggtgtcagtg tttctccgag
    17461 gaaaatcatc ccggaaacgg cagccagcgc cggttccatg ctcatcagcg taccaaatgt
    17521 ccgtgttggc aaacgggtga gggcaatcat ttccagcgaa taaggcagag cggtcgagag
    17581 aatagcgacc gccagaccca atggaataac cgaccagtgc cagagtgctt caccagcctg
    17641 aagcgctcca attggcacga aaattaacgc tgcaatcaac gaaccaattg ccaccgttgc
    17701 agggccatgt tccgctcctg cgcgttgccc acttaaaatg taaatagccc aacaagcccc
    17761 ggcccccagt gccagcgcac agccggttaa atcgacatgg gaaacgtctt gccccagagg
    17821 tagcaggaac cacagaccaa gaaccgccag cacaacccag acgaaatcta ccgggcgacg
    17881 agaagagaac agcgccaccg ccagtggtcc ggtgaactcc agcgccaccg caatacccag
    17941 cggtaccgtc tgaatagaaa gataaaaaag ataattcatc ccccccagcg aaacgccgta
    18001 aaacaacagc ggtaatcgtt gctctttggc aaagcgcagt cgccatggct taaagaacgc
    18061 gatgaggatc agcgttccta atgccagacg cagcgcagtg acacccggtg cgcccaccag
    18121 aggaaaaagt gacttagcta acgaagctcc accctgaata gacgccatgg caacgagcaa
    18181 tatgactatt ggtaaccaga ccggcatttt acgtaatgaa ccaggcatcc tttctcccac
    18241 aaatatctag acttaagtaa agcgtggagt gtactggata tacccaatgc tggttgagca
    18301 tttgttgaaa aaattttccc ccgttttgac taaaatgcgc caggattgat ggaatcatta
    18361 gtctggtgat taggaataat ctggatgaat gacagggaaa acatgcgtaa tacttacgca
    18421 gttctctgaa aaagtgattt aaatttagat ggatagcggt gtatggaaac gttctgttac
    18481 atgaaatggc ccgttagaca tcacaaatcg cgaagagttt cccattaatt tttgatatat
    18541 ttaaaactta ggacttattt gaatcacatt tgaggtggtt atgaaaaaaa ttgcatgtct
    18601 ttcagcactg gccgcagttc tggctttcac cgcaggtact tccgtagctg cgacttctac
    18661 cgtaactggc ggttacgcac agagcgacgc tcagggccaa atgaacaaaa tgggcggttt
    18721 caacctgaaa taccgctatg aagaagacaa cagcccgctg ggtgtgatcg gttctttcac
    18781 ttacaccgag aaaagccgta ctgcaagctc tggtgactac aacaaaaacc agtactacgg
    18841 catcactgct ggtccggctt accgcattaa cgactgggca agcatctacg gtgtagtggg
    18901 tgtgggttat ggtaaattcc agaccactga atacccgacc tacaaacacg acaccagcga
    18961 ctacggtttc tcctacggtg ctggtctgca gttcaacccg atggaaaacg ttgctctgga
    19021 cttctcttac gagcagagcc gtattcgtag cgttgacgta ggcacctgga ttgccggtgt
    19081 tggttaccgc ttctaatcac tttggtgata taaaaaaatc cgcctctcgg ggcggatttt
    19141 tgtttttaag gtttcgggtc gaaaatatcg gttccgagat ggttgtaatc caccttctgt
    19201 aactggaagt tggtgatgta tatcggcccg gctttcttct cagatataaa cgggtatttg
    19261 atgttaattt ccttcgcttt aattcccgtc cactgggaga aaaagcctaa gaagtcattg
    19321 gctgagcggc gggctttaat cacacgatgc gctttatcgt cgctggaaat gaccataaaa
    19381 ggcacctgga aattttgctg atatttatca tcatgggcaa ggtattgcac gtctttgccg
    19441 cgctctttaa aggccagacc gtggtcagaa aagtaaacca gcgagaagct gctgccgctg
    19501 ttgcgtaact gatcgtacag cttgcgcagt aaatcgtccg tttgcgtcat agtatagaga
    19561 tagcacgacg tttctttcga ttgcacaaaa gtttcatatt tgccttgtgt ccggtcgcag
    19621 gcctgcggat gcgagcccat cagatgcaga acaatcagct gcggttgcga gtgctcttgc
    19681 gccagcactt gagcggtcat atccagtaac gcttcgtctt tggtgttttt atctgcttca
    19741 aaattaccat ctttaaggaa gtacacttca tctgctcgtt tggcgatgct ggcgatggcg
    19801 gtatcgtatt cgccgatttg gccctggttg gaaaaccacc aggtctggaa gcccgcgcga
    19861 tttgccaggg tgacaaagtt atcctgaaac tgtggcttgc catcgacaac gcgattgagc
    19921 gttaagccaa gcgatttctg cgtggagcca ctggcggcaa tgtagtcagc aaatatcaaa
    19981 ccgttaacgc tgctggcaaa cggggtattg tcccagtgac cgccaaaggc accgagggca
    20041 tcgcgacgcg cgctttcacc gatcaccacc acataggtct gatacttcgg cttaacggca
    20101 gtgaccgtcc aggtatcttt cattccggaa agttttgcca tccgttcttg ctcttcaatc
    20161 acctcgttat tattgacgat gacatcctta gcaaaacgaa ataccggata gccagtatct
    20221 ttcagtttaa atacgccacc ccaggccagg ttttgcacgg gagcaacaaa aaatgtcgcc
    20281 acactgaata ccagacacag gctgtcgaag gtattccagc gcgctttttc attctctttt
    20341 tttcgcctga ttgctattac gccgagcgca aaaataaata agccgaccag gtagctgtac
    20401 cacggaaaaa tcgtcaggat ttcggtcgac tcttccatat tggtggagtg caatgccagc
    20461 aatgtattga aattaggcgc gccgtaggcc tgagcaaaag ggaaatagca agccgccacc
    20521 agcgaactga caccgaccag aactttttgt acgcgaggca atgttcgcca tagcaaaagc
    20581 aaaatagccg taaacgcagc ggtgtagagc aaactgaagg gataacctaa gccgaggtta
    20641 attaatagcg actgtaaaaa gtagaacgca gtccacgggc taaatacccg gctacgggta
    20701 acaagcgatt ctttgagggt taaattcata tgccactatc gtaacaaacg ccatgtgctt
    20761 accctggcgt caagggtcaa tacctgcaag agtgcgtgaa gaggggaaag gggtccgcat
    20821 ccgcgagccg caatatacgc agggctgcaa gaagatagag cgagccgccg ttcaggtcaa
    20881 ctactgacga acaaatcttt tgcgaagggg attgcaaatc cgacaaaaag tggggttttt
    20941 cagaccaaaa cgcgttatgc ctgcatgaaa gcaggagtga agcggcgtac cgcgacgccg
    21001 ctatttatcg gaaggtttat cttgctgcgg tttgttgttg accatatcgc acaacataga
    21061 gagcagcatt aaccgtactt taaagggaga atgactaaac acgcgcatac acctcttgaa
    21121 ctcattcata agacctcctg acttgctaat cccgtcgatc cttgagggat gattgcatta
    21181 catacagata tagcacaggc tatattatat agctattgct aaaacgttaa ttttttgtac
    21241 cttcgcaact ctggtttaca atgtgcgcac gaaatgagaa cgctatgtat gcgtcaccat
    21301 aatgaggaag cacaatgagt cgtcgcgcag gtacgccaac agcaaaaaaa gtgacgcagt
    21361 tagtgaacgt ggaagagcac gttgaagggt tccgccaggt cagagaggcg catcggcgcg
    21421 agcttattga tgattacgtt gagctgattt ctgacttgat cagggaagtg ggggaagctc
    21481 gtcaggtaga catggctgct cgtttgggag tttcgcaacc gacggtggct aaaatgctta
    21541 agcgactggc aaccatgggg ctgattgaaa tgatcccctg gcgaggcgtg tttttaacgg
    21601 cagaaggaga gaagctggcg caggaaagcc gcgagcgaca tcagatagtc gaaaatttct
    21661 tattggtgtt gggcgtcagt ccggaaatcg cccgtcgcga cgcggaaggc atggagcacc
    21721 atgttagtga agagacgctg gatgcctttc gtttgtttac ccagaaacac ggtgccaaat
    21781 gagcctgcct tttttacgca cgctgcaagg ggatcgtttt tttcagttat taattcttgt
    21841 tggtatcgga ttaagctttt tcgtgccctt tgcaccgaaa tcctggcctg ctgctatcga
    21901 ctggcacacc atcatcacat taagcggcct gatgctgctg accaaaggtg tggagttaag
    21961 cggttatttt gatgtgctgg ggcgcaaaat ggtgcgccgc tttgctacgg agcgtcggct
    22021 ggcgatgttt atggtgctgg cggcggcgct gctttctacc tttctgacca acgatgtcgc
    22081 gctgtttatt gttgttccgc tgactatcac gctaaaaaga ctgtgtgaga tcccggttaa
    22141 tcggctgatt atttttgagg cgctggcagt caatgctggt tcgctactga cgccaattgg
    22201 caacccgcaa aatattctta tctggggacg ttctggtctt tcgtttgccg gatttattgc
    22261 ccaaatggca ccgctggctg gtgcaatgat gctgacactc ctgctgttgt gctggtgttg
    22321 tttccctgga aaggcgctgc aataccatac gggggtgcaa acgccggagt ggaaaccgcg
    22381 gctggtgtgg agttgtctgg gactgtatat cgtctttctg acggcgctgg agttaaaaca
    22441 agagctgtgg ggactggtga ttgtggcggt gggctttgcg ctgctggcac gtcgcgtggt
    22501 gttgagtgtg gactggacgc tgctgctggt gtttatggcg atgtttatcg acgtccattt
    22561 actgatccag cttccagcgt tgcaaggcgt gttgggtaac gtgagtcatt tatctgaacc
    22621 cgggttatgg ttaacggcaa tcggtttatc gcaggtgatc agtaacgtgc cgagtaccat
    22681 attgttgctg aactatgtgc cgccgtcttt attactggca tgggcggtaa acgtaggtgg
    22741 ctttgggtta ttacccggat cgctggcaaa tttgattgcg ctacgtatgg cgaacgatcg
    22801 ccgcatctgg tggcgtttcc atctctattc aataccgatg ctgttgtggg cggcgctggt
    22861 gggatatgtt ttgttggtta tgatcccggc ctggtaaggt ctggcaaata aaaaaggcgg
    22921 attatgagtc cgcctttgtt ttacgttttg acgattaatt cagacgaacc ggcatcccgg
    22981 agcggttttt aatcgcttca tcaagaacaa cctggtcaac atctggctga ccggtcactg
    23041 tctgcacgct cttcgtcagg gtaattggca caatttcctg accttcaaac tgggcttcgg
    23101 tggtagacag cggattatgg acttcaatat aacggctgcc gtctggctcg gtggtcgctt
    23161 ttaccggctc atcaataaac tgtacgcggg taccgaccgg tactttctcg aacaggaatt
    23221 tgatgtcttc gttacgcaga cgcacacaac catgacttac acgcaggccg ataccgaagt
    23281 tggcgttggt gccatggata gcatacaggc gaccgatgta gagtgcatac agccccatcg
    23341 ggttatccgg acctgccgga acgacagccg gaagcggttc gcccgcagcg cggtactctg
    23401 cgtgcatttt ggcggtcggc gtccaggtcg ggcctgcttt cttacgctca actttggtgg
    23461 tccagttgat aggcgtatct ttgcctaact gaccaatgcc gatcggcagc acgataacgg
    23521 tgttggtccc tttcggatag taatacagac gcatctctgc actgttaatg acgatgcctt
    23581 catgaacggt atccggcagg atcagctgct gcggaatgtt cagtacagta ccgcctttcg
    23641 gcaggaaggt atccacaccc gggttcgctt ccatcatatt ggaaagcccc atctggtact
    23701 ccgcggcaaa atactccagc ggctgagtgt taccttcagg aatggtgatc acctgattct
    23761 gaccaaccag gcgactcccg tcggttggca gaggataagt taccgcagag gcggtactgc
    23821 aaaagccgac aacagcgaag gccgctgcga ataatgtttt caatttcata ttcatgttaa
    23881 gcgagatttt gtgcctggca ggccattggg ttgagaatat tagagtattg gaagcgcatt
    23941 ataagttcat tccagctcac agtgaaatca gatgtgtacg aaatcacatt ttttgccttt
    24001 ggcttgagtg tagaccttaa gcgaggagca ggatcttctt tcagacttat ggcataatgc
    24061 gcggtttgtc atatctcttt tcaggatacg cctgtgttag tttccagtaa cgtcaccatg
    24121 cagttcggca gtaagccgtt gtttgaaaac atttccgtca aatttggcgg cggcaaccgt
    24181 tacggcctga ttggcgcgaa cggtagtggt aaatccacct ttatgaagat cctcggcggc
    24241 gacttagagc cgacgttggg taacgtttcc ctcgatccca acgagcgcat tggtaagctg
    24301 cgtcaggatc agtttgcctt tgaagagttc actgtgctgg atacggtgat catggggcat
    24361 aaagagttgt gggaagtgaa gcaggagcgc gaccgcatct atgctttgcc ggaaatgagt
    24421 gaagaagacg gctataaagt ggccgatctg gaagttaaat acggcgaaat ggacggttac
    24481 tctgcggaag ctcgcgccgg tgaattgttg cttggcgtgg gaattccagt ggaacagcac
    24541 tacggcccga tgagtgaagt tgctcctggc tggaagctgc gtgtgctgct ggcgcaggcg
    24601 ctgtttgctg atccggatat tctcctgctc gacgaaccga ccaacaacct cgacatcgac
    24661 accattcgct ggctggaaca ggtgctgaac gagcgtgaca gcaccatgat catcatctcg
    24721 cacgaccgtc acttccttaa catggtttgt acccacatgg cggatctgga ttacggcgag
    24781 ctgcgcgttt atccgggcaa ctacgatgag tacatgacgg cggcgaccca ggcgcgtgaa
    24841 cgtctgctgg ctgataacgc caagaagaaa gcgcagattg ctgagctgca atctttcgtt
    24901 agccgcttta gcgccaacgc ctcgaaatct cgccaggcaa cttcgcgcgc gcgccagatt
    24961 gataaaatca aactggaaga ggtgaaagcc tccagccgtc agaacccgtt catccgtttt
    25021 gaacaggata agaaactgtt ccgtaacgcg ctggaagtgg aaggtctgac caaagggttt
    25081 gataacggtc cactgtttaa aaatctcaac ctgctgctgg aggtcggtga aaaacttgcg
    25141 gtactgggta ccaacggcgt cggtaaatca acgctgctga aaacgctggt gggtgatctg
    25201 caaccggaca gcggcaccgt aaaatggtct gaaaacgcgc gcattggtta ctatgctcag
    25261 gaccacgaat atgagtttga aaatgatctg accgtgttcg aatggatgag ccagtggaag
    25321 caggaaggcg atgacgagca ggcggtacgc agcattctcg gtcgtttgct gttcagccag
    25381 gacgacatca aaaagccagc taaagtgctt tccggtggag aaaaagggcg gatgcttttt
    25441 ggtaagttaa tgatgcagaa gccgaacatt ctgatcatgg acgaaccgac caaccacctg
    25501 gatatggaat ccatcgagtc actgaacatg gcgctggaac tgtatcaggg cacgctgatc
    25561 tttgtttcac acgaccgtga gttcgtaagc tccctggcga cccgcattct ggaaatcacc
    25621 ccggaacgcg tgatcgactt tagcggtaat tacgaagatt acctgcgtag taaagggatc
    25681 gagtaagtta ttatccgtca ccccgatact gtcggggtga cggcggctga tgacaaatgc
    25741 aaaatagcct gatgcgctac gcttatcagg cctacgcagt acctgtaata tattgaatct
    25801 gcaggacttt gtaggccaga taaggcgtta acgccgcatc aggcattaag aaagcgcact
    25861 tgtcagcaat ctgagtaacg tcgaatcgat tttcttaatc ccacatcaat tcacttccat
    25921 taattctcaa acatccatgc ccagtgcgcg cttaccgtga atgttgaggt cggcaagcgt
    25981 aaagcgtcct tcccagtttg tttcatagtc ctcgcgcggg aagtcgcccg gcgatgcgcc
    26041 tttttccagc gccattttga ctttgtgtgc ataagcccga tttttctcgc acattggcgc
    26101 agcagggata tacatcacgt tgccccaacc ctgttgattg tctactggcg cgacggagtg
    26161 aatcacatcg cagtgccacc agacggaatc ccctgctgtt atcgcgggaa tggaagtcag
    26221 cgctttaagc agtagtggat gccatttctc tgaaatgggc agaactttgc caggtgcaac
    26281 cccgcatagt tcatcgtccg gtacgtcatc cagcagcggg cgcaataaaa catacgccat
    26341 cgcttccggg ataggcacca catgtaacaa tccctgaccc gataacatat cagagagcgc
    26401 cgtccagcct tgaaacgtgc ggaacaccga acattttgta gtgttatcta ccgtgtactc
    26461 ttcaacttcc gtacgatgtg ccgcatgcca gggatcgtat ttcgccagat tgccattaaa
    26521 gacgttagcg aaaacgtgct gatacgctgg aagcagccag cgttccagtg ccccggagtc
    26581 ggtatgcgct ccaagacctt tggaggtcgt tccgggcgga cggcggcgga tacggtcagg
    26641 gtagatgacg ctcacatccg ggttaaacca ttgctttcca tcactttcaa atgtccacag
    26701 acgattgaga aacgactgcg cattcgccat ttcttcactc tggcgagcct gcatttgcgc
    26761 ctgcgaccag tagatggggt aaatctcggg acgtgaagcg ctgagcgtcc cgaagaaatt
    26821 atcgccgggg cctttgtaga cctcgtcaaa gcggttgcgg tccagatagt ccagcatcga
    26881 ctgatcccag cctagcgctt gttcgcgggg gaaatggcct tttatcaccg cacaaccgcg
    26941 acgtttaatc tgttcgcgct gctctgcagt aacatgacct gctttgatat cggcataaga
    27001 cagcaccggc cagacggcat cgccctgtgc tttgagtgcg ttgatttcag ccactcgcgt
    27061 ggcaatgtca tcgcttagct gattaaagat ctgctggacg tcgccaagct gcgcccgcag
    27121 cgcgtgcttc atctgacgga tagctgcttt gtgatcggca ggcaatgtgt cgctggtaaa
    27181 agtaggagcc ataaccacct cgcattaact ttcattcgaa actgtgctga tttgcatgtg
    27241 acaatataag ttataaataa gttaatgcaa gtttaatgat ttgatgtgtt ggatgtaaaa
    27301 agaagggaaa accggagcgc atggctccgg tagagaggtc agctgttaaa aggggatgtg
    27361 ttatccagca cagcctgaat cacattcagc gcgccttcat gattattatc atcggtagcg
    27421 taacgggcga tttgtttaat gttttccgca gcattgccca tcgcaaagga ataacgcgcc
    27481 attttcagca tctccgcatc gttaccgctg tcgccaatcg ctaccacatt ttgcggtgac
    27541 agatcccagc gtttcagtaa ccgcgaaata ccgtttgctt tatgtagacc gggaataatc
    27601 aggtcgataa agccaaaacc actggtgacg ggtttcataa tgccatccag cgctatgtgc
    27661 agtttgtcga tcactaacgg gatttgttca tccggcaggt tgagcgaaaa cttgaacagt
    27721 acgtcgtcaa tctcctgata atcttttaca ggtttcaggc gatggtagtg ttttgccatc
    27781 agtgcgacga atgcttcggg ggcgttttcg ctgacatatg cactttgcag accgcaggcg
    27841 acaaaattga gttgcttatc ttttagcaac tcgccaatca caatccgcga ttcatgtcgg
    27901 gtcagttcgc cgtggaacag ttgcttgcca tgttcgtaaa ccagtgcgcc gttttccgcg
    27961 acaaaagaga tctcatcctt tagctcagga aagaacgaaa taagctggta atactgatta
    28021 ccgctggcga caacgaactt aatgccgcgc tttttcagtt cctgatattg cgccataaaa
    28081 cgtggttggt tgtacgtttt ggcgtcgtta agaaaagtac cgtccatgtc tgtgacgata
    28141 acttttacgc tcataggtgt gctcctggct cgaaaatgaa accgtaacag tgtaataaca
    28201 atgtgacgca gagcacaaat tatatttcga atgaaagtaa ggatgaaatt gatgatgtga
    28261 atgatttagc ccggcgacga cgccgccggg ccgaggagat tacagcatat gttcagtacg
    28321 ggcgatgata tcgtcctgtg cgtctggaga gagcgcggtg aagaaggcgg aatagcccgc
    28381 gacacgcact accagatcgc ggtattgatc cggatgtttt ttcgcctcca gcaatgtttc
    28441 gcgggaaacg atgttgtact gaatatgcca gcctttatgc acctcgaaga aggtgcgcag
    28501 caggatcatc agtttctgct tgtcagattc gttctccagt gtggctggat tcagtttctg
    28561 gttgagcaac acgccgccga gaatcgctgc cgtaggcagt ttacccaccg agccaatgac
    28621 cgcagtaggg ccaagatgat cagtaccgga agccgggctt gcgccttctg ccagcggggt
    28681 atgcgcttta cgtccgtccg gcgttgccat agtctgcgcg ccaaacggta cgttagcgga
    28741 gatagacgac gtacccgcgt aatagttgcc gccaaccgga ccacgaccgt agcgcgggtt
    28801 atggtactgt ttcagttcgt cgatataggt ctgataagcg cgagccagca gcgtatcgac
    28861 ggtatcatcg tcgttgccgt acttcggcgc gccgttaatc agccgctgac gcagttgctc
    28921 atgagtcagg ccatcgaagt catccgccag tgcggcagcc agctgttgct gaccaatcgc
    28981 gccctgttca aacaccagtt tcttcactgc cgccaggctg ttgccgaggt tggcaatgcc
    29041 aacctgcagg ccagaaaccc agtcatattt cgcgccgcct tgcttgatac ttttcgctcg
    29101 ctcaatacag tcatccacca gcgccgagca gagaatatcg tgcacgttct cttccagcat
    29161 ggtgtcgacg acatattcga tttcgattga tttacgcgtg tagtaacgga tttgcgtatc
    29221 ccacgcgtcc atcacttcat cgaagttgtt gaagttacct gccgacaacg ctttttcttg
    29281 tggcaggaac actttgccgc tggtggcatc acgcccgcct tccagcgccg ccagcatcac
    29341 gcgggcgaag ttgataaagc tcatgccggt acagcgatag ccccatttgc caccgacggc
    29401 ggtttcgata caaccaatcg ctgcatagtc gtaagcgtcc tgcggttcaa taccgagttt
    29461 aataaattcc gggatgacga tttcgtcgtt gttgaacgcc ggcatcccga agccgcaacg
    29521 gatcacctgt acgcaggcgt cgaggaaatc gttactcatt cctgcatgga aacgcacgct
    29581 gaggttaggc tgagtggaac gcaggcgacc gcaggattca aggatcgcgt aagagagggg
    29641 attcaccgcg tccattggtt gaccatcaac cagattttgc ccgccaatgg tgacgttctg
    29701 atacagcgga cttcccgcag aggcttttga gtgtgagccg gagcggattt tgttcacttc
    29761 cagcagtttc agccagcagc tatgcagcaa ttcgatggcg tgttcgcgat ccagtgtctg
    29821 gttgagttca acgtcgcggc gatagtaagg gtagagatac tggtccatac gaccaaacga
    29881 taccgagtga ccgttagatt cgatctgcaa tatcaactgg atgaagtaac acagttgcag
    29941 cgcctgccag aacgtctgcg gcggctggtg ggcgataaga tcgcagtttt ctgccatcgc
    30001 cagcagttca tcgcgacggc tttcgcgggt ttcggtcgcg gccatttcac gcgccagggc
    30061 agcgaaacgt tcaatgtgtt cactgactgc caccagcacg atatcaattg ctttcaggaa
    30121 ctgctcgccg tgtaaatctt ccagcaccgt caggttgatg cgcgagcgac gttccgctac
    30181 tttctcgcgc agaccatcaa gccctttttc cagcagcagc gggaaattga ccgccaggtg
    30241 cgcatcgccg gaggtcatat tgccttccgc tttaatgatg ccggtcgcca gcagaccttt
    30301 ttgctcatcg gtaaacatgc cgtagcagcg atcctgtacg gtctgaccgc gccaccacgg
    30361 gcacacttca tgcagaacgc gtttgttctc ttcgctcacc gcaaagccag caccgggacg
    30421 atctgccaga tcatcaatct ctttttcgat ccacgagaca gtatattccg ggaagatcgg
    30481 cgcggcgcga acttcgcttg cctggttgcc gatgatcaac tcatcgtgct tgatccagat
    30541 agtgcgattc gccaggtgat gtgccagcgc cagcgcgcga cgtacaggga tcggcttatc
    30601 gagatgttgt tgatacatct cggtatagtg ctgcgcgcgc tcggtacaaa ccggcggttt
    30661 cacaatatgc accagcgcat ttttgtgcgc tttaatgcgg tcgctgagcg tgtccagttt
    30721 cagtgtggtc atggttgtta tcctcgtaag gtcgcggtta accctttctg gcaggcatac
    30781 tgctgggcaa agtcgagcag ttctggcgca tcaagcggtt tttccggggc gtcatagggc
    30841 agattaagta agtgatattt gttgatgccc agcgtgtggt agggcaggaa atgaatttcg
    30901 ccaacgtgca gctcgtcggc ggcaaaatcg gtaatggctt ttacagaggt ttcgtcggca
    30961 ttaaagccct gaatcagcgg cacgcggata atgatttttt tgcccgctgc ggcgagtttt
    31021 ttcaggttat ccagcactct ggcggcgtta ccgtcggtcc actgtttaaa cggcgcgtcg
    31081 gcaacgtgtt ttaaatcggc aagaaacaga tcgatatagg gcagagaagg ggcgatatat
    31141 ttccacggca catgcagaca ggtttctacc gcagtatgaa tgccggcctc gtggctggct
    31201 tgcagtagcg ccgtcgccat ttccggctgc ataaagggtt caccacccga aagcgttaag
    31261 cctccgccgc tgcgatcgta aaacggttta tcgcgcaaaa cggtcgccat gatctcctca
    31321 acgcttttca cttcaccaca cacggttaat gcctgtgtcg ggcagcagtc ggttaacgcc
    31381 gtcagatgct ccggggttaa cttttcccga tgaataagca aaccattcag cgcgcgctca
    31441 atcacttccg gcgctgcctt agcgcacagc tcgcagcctt ccagacacag tcgtgcgtca
    31501 tacagcagat cctgcgtgcg ggcgcggctt tccgggttct gacaccagcg gcagcccagc
    31561 gaacagcctt taagaaatac gaccgtgcgg ataccggggc catcatgggt cgagtagcgc
    31621 tgaatattga aaatcatagt tgcctctctg tttcgttcaa acattaaaat actttcgaat
    31681 gaaagttaga ttgatgtgcg tcaactgttc agagagtttt cccgtgatag tctacattca
    31741 gacaaaaagt acattttgag gatggttatg gaactgtatc tggatacttc agacgttgtt
    31801 gcggtgaagg cgctgtcacg tatttttccg ctggcgggcg tgaccactaa cccaagcatt
    31861 atcgccgcgg gtaaaaaacc gctggaggtt gtgcttccgg aacttcatga agcgatgggc
    31921 ggtcaggggc gtctgtttgc ccaggtaatg gctatcactg ctgaagggat ggtgaatgac
    31981 gcacgtaaat tgcgttctat tattgcggat atcgtggtga aagttccggt gaccgccgag
    32041 gggctggcag cgattaagat gttaaaagcg gaagggattc cgacgctggg aaccgcggta
    32101 tatggcgcgg cacaagggtt gctgtcggcg ctggcaggtg cggaatatgt tgcgccttac
    32161 gttaatcgta ttgatgctca gggcggtagc ggcattcaga ctgtgaccga cttacaccag
    32221 ttattgaaaa tgcatgcgcc gcaggcgaaa gtgctggcag cgagtttcaa aaccccgcgt
    32281 caggcgctgg actgcttact ggcaggatgt gaatcaatta ctctgccact ggatgtggca
    32341 caacagatga ttagctatcc ggcggttgat gccgctgtgg cgaagtttga gcaggactgg
    32401 cagggagcgt ttggcagaac gtcgatttaa ccggacgttc tgcatcctca taaattgctg
    32461 atgacgtggc ggagtgccgc gtcttatcag gcctggaggt ggcaattact gcccgcacac
    32521 ttcacacccc ggattgcgca tcagtttcat ttcgcgaaac tgacaggtca tcgcatcgta
    32581 catcacgatt tttccgctgg ccggtttgcc ataacctgcc agcagcttga tcgcttccat
    32641 tgcctgcaac gaaccaatta cgccgatcaa tggtgccatc acgcctgctt ctacgcaggt
    32701 taacgcgttc tcgccaaaca aacggctaag gcagcgatag cacggttcgc cgtcctgata
    32761 agtgaagacc gtgatttgac cttccatacg aattgccgcg ccggaaacca gcggcacctt
    32821 cgcggcaaaa catcctgcgt tcagttgatt acgtaccgca acgttatccg tacagtcgag
    32881 caccagatcg tgttcagcaa tcaatgctgc aagttctgcg tcatccagca gtgcattgac
    32941 tggcgtaatc gcgatatgtg ggttgatccg cgccagggcg tcacgggcgg attccacctt
    33001 cggttgcccg accgtggcat cactgtgcag cgtctggcgt tgcagattcg agagcgaaac
    33061 cgagtcgaag tcgagcagcg tcaggttgcc gacaccggcg cttgccagat actgcgaagc
    33121 tgcgcagccg agtccaccga ggccgactac cagcacacga gaatctttta gcgcctcctg
    33181 accgtcaaaa tcaaaaccgc gcagaatgat ttgccggttg tagcgcagca tctcctgatc
    33241 gctgagttcc gccattacag gcctccgaac aacgcgttaa acggttctac ttccacccat
    33301 tcgcccacat ccacattgcc gcgatcgcgt tccagtacga taaagcagtt gccgaggcta
    33361 aaggagctaa atatatgtga accctgatgt ccagtggtcg tcacttccag ttcgccatcg
    33421 gcgttgcgtt gcagcacgcc gcgctggaaa tcaaggcgac ctggcgtttt tttcaggcgg
    33481 gacgctgtgc gtacgcgctg gcgcgcgggc aggccgctgg cggtgttacc gcttagtttt
    33541 gccagcaaag gctgtaccag ttgatagaag gtcagcgtcg ctgaaactgg gttgcccggc
    33601 aggccgcaga accagctatt gctgagttta ccgaacgcga acggtttacc tggtttaatc
    33661 gccagcttcc agaaggcgat ctcccccagc tcttcaagaa tcgttttggt gtaatccgct
    33721 tcacccactg aaacaccgcc ggaactgatc accacatccg cctggctgtc ggcttcaata
    33781 aatgcggcgc gcagggcatg gggatcatcg cggataatcc ctaagttaat tacctcgcaa
    33841 cccagctgtt ctaacatcag gtgtacggcg agacggttgg tatcgtagat ttggccgtcg
    33901 cccagcggct gaccgggcaa ctggagttca tcaccggtag aaaaaagcgc cactcgcact
    33961 ttacgaatca ccggaacttc ggcaatcccc agtgaagcaa ttactggcag ctctgcggta
    34021 gtcaggcgag tccccgccgg gaaaacaacc gcacctgcag agatatcttc accgcgacgg
    34081 cgaatatttt gcccgctacg cacttcagca gtaaaacgca cgccattgtc cgtttgttca
    34141 gtctgctcct gcatcaccac cgcttcgcag ccttccggca ccggcgcacc ggtcataata
    34201 cgaatgcagg tacccgcagg ccattcacca tggtacggct gaccggcaaa ggatttaccg
    34261 gcaacgggca gcggttgccc ggaggcaata tcggctaaac gcaccgcgta gccgtccatt
    34321 gcggagttat caaaccccgg aacatcaagc ggcgaaacga catcgctcgc cagaatacga
    34381 ccaaaacact gtaccagtgg cagcgtttcc tgggcggtca gtggggtgac gcgagaaagc
    34441 atctcattaa gcgcggtgtc gagcgacatc aatccggtgg taaattccat gaaaacactc
    34501 ctgcggaggc aaaatcgaat ttgcctatta tgtcagaaaa acgccacaga ctgtatgcca
    34561 cctcgggcgt agcgctgggt cctgccttta catgccatat ccatctttct atattcaaaa
    34621 attgaatgag taattcataa aaattctgat atttatagca aaagtggcga gccaccctta
    34681 atggacgaat actatgggca aagcagtcat tgcaattcat ggtggcgcag gtgcaattag
    34741 ccgcgcgcag atgagtctgc aacaggaatt acgctacatc gaggcgttgt ctgccattgt
    34801 tgaaaccggg cagaaaatgc tggaagcggg cgaaagtgcg ctggatgtgg tgacggaagc
    34861 ggtgcgtctg ctggaagagt gtccactgtt taacgccgga attggcgccg tctttacgcg
    34921 tgatgaaacg catgaactgg acgcctgtgt gatggatggt aacaccctga aagccggtgc
    34981 ggtggcgggc gttagtcatc tgcgtaatcc ggttcttgcc gcccggctgg tgatggagca
    35041 aagctcgcat gtgatgatga ttggcgaagg ggcagaaaat tttgcgtttg ctcatggcat
    35101 ggagcgcgtc tcaccggaga ttttctccac gcctttgcgt tatgaacaac taatggcagc
    35161 gcgcgaggaa ggggcaacag tcctcgacca tagcggtgcg ccactggatg aaaaacagaa
    35221 aatgggcacc gtgggggccg tggcgttgga tttagacggc aatctggcgg cagccacgtc
    35281 cacgggcgga atgaccaata aattacccgg acgagttggc gatagcccct tagtgggtgc
    35341 cggatgctac gccaataacg ccagtgtggc ggtttcttgt accggcacgg gcgaagtctt
    35401 catccgcgcg ctggcggcat atgacatcgc cgcgttaatg gattacggcg gattaagtct
    35461 cgcggaagcc tgcgagcggg tagtaatgga aaaactccct gcgcttggcg gtagcggtgg
    35521 cttaatcgct atcgaccatg aagggaatgt cgcgctaccg tttaacaccg aaggaatgta
    35581 tcgcgcctgg ggctacgcag gcgatacgcc aaccaccggt atctaccgtg aaaaagggga
    35641 cgccgttgcc acacagtgat gaacttgatg ccggtaatgt gctggcggtt gaaaatctta
    35701 atattgcctt tatgcaggac cagcagaaaa tagctgcggt ccgcaatctc tcttttagcc
    35761 tgcaacgcgg tgagacgctg gcaattgttg gcgaatccgg ctccggtaag tcagtgactg
    35821 cgctggcatt gatgcgtctg ttggaacagg cgggcggttt agtacagtgc gataaaatgc
    35881 tgttgcggcg gcgcagtcgt gaagtgattg aacttagcga gcagagcgct gcacaaatgc
    35941 gtcatgtgcg cggtgcggat atggcgatga tatttcagga gccgatgaca tcgctgaacc
    36001 cggtatttac tgtgggtgaa cagattgccg aatcaattcg tctgcatcaa aacgccagtc
    36061 gtgaagaagc gatggtcgag gcgaagcgga tgctggatca ggtacgcatt ccggaggcac
    36121 aaaccattct ttcacgttat ccgcatcaac tctctggcgg gatgcgccag cgagtgatga
    36181 ttgcgatggc gctgtcatgc cgcccggcgg tgctgattgc cgatgagcca accaccgcgc
    36241 tggatgtcac tattcaggcg cagatcctgc aattaatcaa agtattgcaa aaagagatgt
    36301 cgatgggcgt tatctttatc actcacgata tgggcgtggt ggcagagatt gccgatcggg
    36361 tactggtgat gtatcagggc gaggcggtgg aaacgggtac cgtcgaacag atttttcatg
    36421 caccgcaaca tccttacacc cgtgcgctgt tagctgctgt tccgcaactt ggtgcgatga
    36481 aagggttaga ttatccccga cgtttcccgt tgatatcgct tgaacatcca gcgaaacagg
    36541 aaccccccat cgagcagaaa acggtggtgg atggcgaacc tgttttacgg gtgcgtaatc
    36601 tggtcacccg tttccctttg cgcagcggtt tgttgaatcg cgtaacgcgg gaagtgcatg
    36661 ccgttgagaa agtcagtttt gatctctggc ctggcgaaac gctatcgctg gtgggcgagt
    36721 ctggcagcgg taaatccact accgggcggg cgttgctgcg cctggtcgaa tcgcagggcg
    36781 gcgaaattat ctttaacggt cagcgaatcg ataccttgtc acccggcaaa cttcaggtat
    36841 tgcgccgcga tattcagttt atttttcagg acccttacgc ttcgctggac ccacgtcaga
    36901 ccatcggtga ttcgattatc gaaccgttgc gtgtacacgg tttattgcca ggtaaagaag
    36961 cggttgcacg cgttgcgtgg ttgctggagc gcgtgggcct gttacctgaa catgcctggc
    37021 gttacccgca tgagttttcc ggcggtcagc gccagcgcat ctgcattgct cgcgcgttgg
    37081 cattgaatcc aaaagtgatc attgccgacg aagccgtttc ggcgctggat gtttctattc
    37141 gcgggcagat tatcaacttg ttgctcgatc tccagcgtga tttcggcatt gcgtatctgt
    37201 ttatctccca cgatatggct gtggtagagc ggattagtca tcgtgtggcg gtgatgtatc
    37261 tcgggcaaat tgttgaaatt ggtccacggc gcgcggtctt cgaaaacccg cagcatcctt
    37321 atacgcgtaa attactggcg gcagttccgg tcgctgaacc gtcccgacaa cgaccgcagc
    37381 gtgtactgct gtcggacgat cttcccagca atattcatct gcgtggcgaa gaggttgcac
    37441 ccgtctcgtt gcaatgcgtc gggccggggc attacgtcgc acaaccacaa tcagaatacg
    37501 cattcatgcg tagataacat tcaggcggag aataaaatgg caagagctgt acaccgtagt
    37561 gggttagtgg cgctgggcat tgcgacagcg ttgatggcat cttgtgcatt cgctgccaaa
    37621 gatgtggtgg tggcggtagg atcgaatttc accacgctcg atccgtatga cgcgaatgac
    37681 acgttatctc aggccgtagc gaaatcgttt taccaggggc tgttcggtct ggataaagag
    37741 atgaaactga aaaacgtgct ggcggagagt tataccgttt ccgatgacgg cattacttac
    37801 accgtgaaat tgcgggaagg cattaaattc caggatggca ccgatttcaa cgccgcagcg
    37861 gtgaaagcga atctggaccg ggccagcgat ccggcaaatc atcttaaacg ctataacctg
    37921 tataagaata ttgctaaaac ggaagcgatc gatccgacaa cggtaaagat taccctcaaa
    37981 cagccgttct cagcgtttat taatattctt gcccatccgg cgaccgcgat gatttcaccg
    38041 gcagcgctgg aaaaatatgg caaggagatt ggttttcatc cggtgggaac cggaccgtat
    38101 gaactggata cctggaatca gactgatttt gtgaaggtga aaaaattcgc gggttactgg
    38161 cagccaggat tgcccaaact ggacagcata acctggcgtc cggtggcgga taacaacacc
    38221 cgcgcggcaa tgctgcaaac cggtgaagcg cagtttgctt tccccattcc ttacgagcag
    38281 gccgcactgc tggagaaaaa caaaaatatc gagttgatgg ccagtccgtc aattatgcag
    38341 cgttatatca gtatgaacgt gacgcaaaag ccattcgata acccgaaggt ccgtgaggcg
    38401 ctgaattacg ccattaaccg tccggcgctg gtgaaagtgg ccttcgcggg ctatgcaacg
    38461 ccagctactg gtgtggtacc gccgagtatc gcctacgcgc aaagttataa accgtggcct
    38521 tacgatccag tgaaagcgcg cgaattactg aaagaggcgg gatatcccaa tggtttcaat
    38581 accacgctgt ggtcgtcaca taaccacagc accgcgcaga aagtgctgca atttacccag
    38641 cagcagttag cgcaggtcgg gattaaagcc caggtgactg cgatggatgc cggacagcgg
    38701 gcggcagaag ttgaaggtaa agggcaaaaa gagagcggcg tgcggatgtt ctacactggc
    38761 tggtcggctt caaccggcga agcggactgg gcactatcgc cgctgtttgc ctcgcagaac
    38821 tggccaccga cgctgtttaa taccgcgttt tacagcaata aacaggtgga tgacttcctg
    38881 gctcaggcac tgaaaactaa tgatccggcg gaaaagaccc gcttgtataa ggcggcgcag
    38941 gatatcatct ggcaagagtc gccgtggatc ccgctggtgg tagaaaaact ggtgtcggca
    39001 cacagtaaaa acctgaccgg tttttggatc atgccagaca ccggcttcag ctttgaagac
    39061 gcggatttgc aataagcaac gcagggagtg gaatgcttaa ttacgttatc aaacgattac
    39121 tggggttgat tccgacgctg tttatcgtct cggtgctggt gtttttattt gtgcatatgc
    39181 tgcccggcga tccggcgcga ttgattgccg ggcccgaagc tgatgcgcag gttatagaac
    39241 tggtgcgtca gcagctgggg ttggatcagc cgctgtatca ccagttctgg cactatatca
    39301 gcaatgctgt gcagggggat tttggcctgt cgatggtgtc gcgtcgtccg gttgccgatg
    39361 agattgccag ccgctttatg ccaacgctgt ggctgaccat aaccagtatg gtctgggcgg
    39421 tgatatttgg tatggcggcg ggaattatcg ccgccgtctg gcgtaatcgt tggccggatc
    39481 gattgagtat gaccattgcg gtgtcgggga tctcgtttcc ggcatttgct ctggggatgc
    39541 ttttaattca ggtattctcc gttgaactgg gctggctgcc taccgtggga gcagacagtt
    39601 ggcagcacta cattttaccc tccctgacgc tcggcgcggc agtggccgcc gtgatggcgc
    39661 gctttacccg cgcgtcgttt gtcgacgttt taagcgaaga ttatatgcgt accgcgaggg
    39721 cgaaaggggt gagcgaaacc tgggttgtcc tcaaacacgg gctacgtaac gcgatgatcc
    39781 cggtagtgac catgatgggc ttacagtttg gctttttgct cggtggttct atcgtggtgg
    39841 agaaagtttt caactggccc ggacttggac gcttactcgt tgactccgta gaaatgcgcg
    39901 attacccggt gattcaggcg gaaattctgc ttttctcgct ggaatttatt cttatcaact
    39961 tagtggtgga tgtgctttac gccgccatta acccggctat caggtacaag taaggatgcg
    40021 actatttaac tggcgacgtc aggcggtgtt aaacgccatg ccactggtca aacctgacca
    40081 ggtacgtaca ccgtggcatg aattctggcg acgatttcgc cgtcagcata tggcgatgac
    40141 cgccgcatta ttcgttattt tattgattat ggtggccatt tttgcacgct ggatcgctcc
    40201 ctatgacgcc gaaaattatt ttgattatga caatctgaat aacggacctt ctttgcagca
    40261 ctggtttggc gtcgattcac tggggcgcga cattttcagc cgtgtcctgg ttggtgcgca
    40321 aatctcgctg gctgcgggcg tgtttgccgt gtttatcggt gcggcgatag ggacgttgct
    40381 gggcttgctg gctggatatt atgaaggctg gtgggatcgg ctgatcatgc gcatttgcga
    40441 tgtgctgttt gccttcccgg gtattttact ggcgatcgct gttgttgcgg tgttgggaag
    40501 cggcattgct aacgtgatta ttgcagtcgc cattttttcc atccccgcgt ttgcccgcct
    40561 ggtgcgcggc aacacgctgg tgttgaaaca gcaaaccttt attgagtcag cacgcagtat
    40621 tggtgccagc gatatgacca ttttattgcg tcatatcttg cctgggaccg tctcgtctat
    40681 cgtggtgttt ttcaccatgc gcattggcac ttcgattatc tctgccgcca gcctgtcatt
    40741 tctcggcctc ggtgcgcagc cgccgacacc agagtgggga gcaatgctca atgaggctcg
    40801 agcggatatg gttatcgcgc cgcatgtcgc tgtttttccg gccctggcta tttttctgac
    40861 tgtactggcg ttcaatttgt tgggcgatgg tttacgcgat gcgctggatc cgaaaattaa
    40921 aggatagtta cgtttgaata ttgcttgaaa gagtaatcac ctcacaggaa attattgccc
    40981 taagcaagtg ttgtaacttt ctgctgattt tgtagaatcg ggtaatttgg ttaaaagccg
    41041 cagcaaggga ctgtttttgc agcggcacag cgttcagaca gttattttgt taaatgtatt
//
LOCUS       DAZGCQ010000037        40852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000037 DAZGCQ010000000
VERSION     DAZGCQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.037"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(68..349)
                     /gene="yehE"
                     /locus_tag="ACHK07_002098"
     CDS             complement(68..349)
                     /gene="yehE"
                     /locus_tag="ACHK07_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO8102428.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(611..1720)
                     /gene="apbC"
                     /locus_tag="ACHK07_002099"
     CDS             complement(611..1720)
                     /gene="apbC"
                     /locus_tag="ACHK07_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO8102429.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1852..3885
                     /gene="metG"
                     /locus_tag="ACHK07_002100"
     CDS             1852..3885
                     /gene="metG"
                     /locus_tag="ACHK07_002100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO8102430.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4026..4220
                     /locus_tag="ACHK07_002101"
                     /pseudo
     CDS             4026..4220
                     /locus_tag="ACHK07_002101"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            6753..7829
                     /locus_tag="ACHK07_002102"
                     /pseudo
     CDS             6753..7829
                     /locus_tag="ACHK07_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7842..10874
                     /locus_tag="ACHK07_002103"
     CDS             7842..10874
                     /locus_tag="ACHK07_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO8102431.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHITWGKVGSNGQSQVKSFAD
                     ATAAEKAELKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNNETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDELLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLFRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLAPYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIGLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKIKNSSKVLPDSALQHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTAGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVAELEDRLAPDLGLDDNGSLLLDFGSRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVE
                     HPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDAITASELINDIEALFE"
     gene            10884..14516
                     /gene="yehI"
                     /locus_tag="ACHK07_002104"
     CDS             10884..14516
                     /gene="yehI"
                     /locus_tag="ACHK07_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO8102432.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRNWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEKSCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQKNRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNNPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRHFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHLLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            14577..14894
                     /gene="yehK"
                     /locus_tag="ACHK07_002105"
     CDS             14577..14894
                     /gene="yehK"
                     /locus_tag="ACHK07_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO8102433.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            16134..17222
                     /gene="yehL"
                     /locus_tag="ACHK07_002106"
     CDS             16134..17222
                     /gene="yehL"
                     /locus_tag="ACHK07_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO8102434.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDAAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17233..19512
                     /gene="yehM"
                     /locus_tag="ACHK07_002107"
     CDS             17233..19512
                     /gene="yehM"
                     /locus_tag="ACHK07_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO8102435.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFLGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESDGKPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHVPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLN
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            19505..20641
                     /gene="yehP"
                     /locus_tag="ACHK07_002108"
     CDS             19505..20641
                     /gene="yehP"
                     /locus_tag="ACHK07_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8102436.1"
                     /translation="MSELNDLLTNRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARNFDFKSTLRANLQHWHPQHSKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVNLTADVADPVELLMKVQ
                     LGGGTSIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20638..22641
                     /gene="yehQ"
                     /locus_tag="ACHK07_002109"
     CDS             20638..22641
                     /gene="yehQ"
                     /locus_tag="ACHK07_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO8102437.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMAQRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSAGVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHMLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22766..23227
                     /gene="yehR"
                     /locus_tag="ACHK07_002110"
     CDS             22766..23227
                     /gene="yehR"
                     /locus_tag="ACHK07_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO8102438.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23268..23738)
                     /gene="yehS"
                     /locus_tag="ACHK07_002111"
     CDS             complement(23268..23738)
                     /gene="yehS"
                     /locus_tag="ACHK07_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO8102439.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23785..24504)
                     /gene="btsR"
                     /locus_tag="ACHK07_002112"
                     /gene_synonym="yehT"
     CDS             complement(23785..24504)
                     /gene="btsR"
                     /locus_tag="ACHK07_002112"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO8102440.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24501..26186)
                     /gene="btsS"
                     /locus_tag="ACHK07_002113"
     CDS             complement(24501..26186)
                     /gene="btsS"
                     /locus_tag="ACHK07_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO8102441.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26408..27139
                     /gene="mlrA"
                     /locus_tag="ACHK07_002114"
     CDS             26408..27139
                     /gene="mlrA"
                     /locus_tag="ACHK07_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO8102442.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            27199..27306
                     /gene="yohO"
                     /locus_tag="ACHK07_002115"
     CDS             27199..27306
                     /gene="yohO"
                     /locus_tag="ACHK07_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO8102443.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27287..28018)
                     /gene="yehW"
                     /locus_tag="ACHK07_002116"
     CDS             complement(27287..28018)
                     /gene="yehW"
                     /locus_tag="ACHK07_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO8102444.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(28023..28949)
                     /gene="yehX"
                     /locus_tag="ACHK07_002117"
     CDS             complement(28023..28949)
                     /gene="yehX"
                     /locus_tag="ACHK07_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO8102445.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVVDYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(28942..30099)
                     /gene="yehY"
                     /locus_tag="ACHK07_002118"
     CDS             complement(28942..30099)
                     /gene="yehY"
                     /locus_tag="ACHK07_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO8102446.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKFGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(30106..31023)
                     /gene="osmF"
                     /locus_tag="ACHK07_002119"
     CDS             complement(30106..31023)
                     /gene="osmF"
                     /locus_tag="ACHK07_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO8102447.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLGRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31275..33572)
                     /gene="bglX"
                     /locus_tag="ACHK07_002120"
     CDS             complement(31275..33572)
                     /gene="bglX"
                     /locus_tag="ACHK07_002120"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO8102448.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSSQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33768..35483
                     /gene="dld"
                     /locus_tag="ACHK07_002121"
     CDS             33768..35483
                     /gene="dld"
                     /locus_tag="ACHK07_002121"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8102449.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35522..36454)
                     /gene="pbpG"
                     /locus_tag="ACHK07_002122"
     CDS             complement(35522..36454)
                     /gene="pbpG"
                     /locus_tag="ACHK07_002122"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO8102450.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36628..37215)
                     /gene="yohC"
                     /locus_tag="ACHK07_002123"
     CDS             complement(36628..37215)
                     /gene="yohC"
                     /locus_tag="ACHK07_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO8102451.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37385..37963
                     /gene="yohD"
                     /locus_tag="ACHK07_002124"
     CDS             37385..37963
                     /gene="yohD"
                     /locus_tag="ACHK07_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8102452.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKIIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38093..38854)
                     /gene="yohF"
                     /locus_tag="ACHK07_002125"
     CDS             complement(38093..38854)
                     /gene="yohF"
                     /locus_tag="ACHK07_002125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8102453.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38907..40343)
                     /gene="mdtQ"
                     /locus_tag="ACHK07_002126"
     CDS             complement(38907..40343)
                     /gene="mdtQ"
                     /locus_tag="ACHK07_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO8102454.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSELFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40567..40650
                     /gene="yohP"
                     /locus_tag="ACHK07_002127"
     CDS             40567..40650
                     /gene="yohP"
                     /locus_tag="ACHK07_002127"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO8102455.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT        10221 a        10733 c        10205 g         9693 t
ORIGIN      
        1 aaaaagccga aatcatttat ataaatgatt tcggctttct aaatgcaatt aaaatcaggt
       61 tgccaaatta atcgtagcga tttaacacga ttcgacgttt gctatcacta cctgcaacaa
      121 ccacttctgt cgttacacct ttgacgggcg aagaaacctg atgacgggta tcgacattat
      181 acgttaactg accacaatgc tgttgaggtt gactattaac catagccata ctgcatgttg
      241 ggggactgat cctgccatta atcgttaatg tggcagtctc tgaagagaac gcgggagaag
      301 ccaatccata ggcaagaaaa ataattcctg acagccaata cttattcatt gaacgttatc
      361 cctgtagtaa aggttatgcc tggatagaat gagtgcataa caaactatag ctgtacatcc
      421 actacacagc acgaaggatg ataatgaagc attgcctgta cgatcaatcg actttgtaga
      481 gtttcggacg aaggtccgca gaatattcgc agtattaaat aagtgttcat taatgcggca
      541 aaaacaaata caccgcatca atataacatc tctgcggtgt atgaagaata accagaatgg
      601 ttaataggcg ttagaccgcg cggaaggaaa tctcgcctgg aatgacttca ccctgccagt
      661 agagctgagc tgcaacgcgg tcagccagct ggcggtagat agcggtaaat tcgctctccg
      721 gacggctgat aaccgttggc gtgcctttat cgagatcctc acgtaaggag atgtgtaacg
      781 gcatctgccc cagcagctgt gtatggtatt tttcagccag tttctctgca ccaccggtac
      841 cgaaaatcgg ttcatggtga ccacagttac tgcaaatatg cacactcatg ttctcgacaa
      901 taccgagcac cggaacctcg actttctcga acatcacaat gccttttttc gcatcgatca
      961 acgcgatgtc ctgcggggtg gtgaccacga ctgcaccagt tactggaatg ttttgcgcca
     1021 gcgtcaattg aatgtcaccg gtgcctggcg gcatatcaag caccagatag tcgagatccg
     1081 gccacaaggt ttcctgcaac atctgcatta acgccttgct cgccatcggt ccacgccaca
     1141 ccatcgcatt gtcgtcagtg accagataac caattgaatt ggttgccagg ccatgagaca
     1201 taatcggtgc catatgggta ccgtccggcg aagttggacg ctgattttct gcgcccagca
     1261 tggtgggaat tgatgggccg tagatatcgg cgtccagaat gccgactttc gccccttccg
     1321 ccgccagtgc cagcgccagg tttaccgccg tggaggattt acccacgccg cctttaccgg
     1381 aactgaccgc aatgatgttc ttcacgccat taatacccgg ctgattcttc acgcgtttta
     1441 aggtggcgat attgtgcgac agtttccagt cgatagcttt cgccccggtg atacgcagca
     1501 gttcggcgct acattgctct tttaactctt caaacgcgct atgccagacg aatggcataa
     1561 ccagttccac atgcaacgtg tcgtccatcc aggcgacatg gtgcaacgct ttcagcgtgg
     1621 tgaggttatg cttcagggtt gggtgctgaa aattggccag tgtcccggcg accattgcgc
     1681 gcaaggcttc tggtgatttg gcctgggatt gttcgttcat cccgactcct tttttgtata
     1741 gataaaccat cagctgatag tttacctgaa gaatatagag aagtacttac ttaacatttt
     1801 cccatttggt actatctaac cccttttcac tattaagaag taatgcctac tatgactcaa
     1861 gtcgcgaaga aaattctggt gacgtgcgca ctgccgtacg ctaacggctc aatccacctc
     1921 ggccatatgc tggagcacat ccaggctgat gtctgggtcc gttaccagcg aatgcgcggc
     1981 cacgaggtca acttcatctg cgccgacgat gcccacggta caccgatcat gctgaaagct
     2041 cagcagcttg gtatcacccc ggagcagatg attggcgaaa tgagtcagga gcatcagact
     2101 gatttcgcag gctttaacat cagctatgac aactatcact cgacgcacag cgaagagaac
     2161 cgccagttgt ctgagcttat ctatactcgc ttgaaagaaa acggttttat taaaaaccgc
     2221 accatctctc agctgtacga tccggaaaaa ggcatgttcc tgccggaccg ttttgtgaaa
     2281 ggcacctgcc cgaaatgtaa atccccggat caatacggcg ataactgcga agtctgcggc
     2341 gcgacctaca gcccgactga actgatcgag ccgaaatcgg tggtttctgg cgctaccccg
     2401 gtaatgcgtg attctgaaca cttcttcttt gatctgccct ctttcagcga aatgttgcag
     2461 gcatggaccc gcagcggtgc gttgcaggag caggtggcaa acaaaatgca ggagtggttt
     2521 gaatctggcc tgcaacagtg ggatatctcc cgcgacgcac cgtacttcgg ttttgaaatt
     2581 ccgaacgcgc cgggcaaata tttctacgtc tggctggacg caccgattgg ctacatgggt
     2641 tctttcaaga atctgtgcga taagcgcggc gacagcgtaa gcttcgatga atactggaag
     2701 aaagactcca ccgccgagct gtaccacttc atcggtaaag atattgttta cttccacagc
     2761 ctgttctggc ctgcaatgct ggaaggcagc aacttccgca agccgaccaa cttgtttgtt
     2821 cacggctatg tgacggtgaa cggcgcgaag atgtccaagt ctcgcggtac ctttattaaa
     2881 gccagcacct ggctgaatca ttttgacgcc gacagcctgc gttactacta cactgcgaaa
     2941 ctctcttcgc gcattgatga tatcgatctc aacctggaag atttcgttca gcgcgtgaat
     3001 gccgatatcg ttaacaaagt ggttaacctg gcctctcgta atgcgggctt tatcaacaag
     3061 cgttttgacg gtgtgctggc aagcgaactg gctgacccgc agttgtacaa aaccttcact
     3121 gatgccgctg aagtgattgg tgaagcgtgg gaaagccgcg aatttggtaa agcagtgcgc
     3181 gaaatcatgg cgctggctga tctggctaac cgctatgtcg atgaacaggc tccgtgggtg
     3241 gtggcaaaac aggaaggccg cgatgctgac ctgcaggcga tttgctcaat gggcatcaac
     3301 ctgttccgcg tgctgatgac ttacctgaag ccggtactgc cgaaactgac cgagcgcgca
     3361 gaagcattcc taaatacgga actgacctgg gatggtatcc agcaaccgct gctgggccac
     3421 aaagtgaatc cgttcaaggc gctgtataac cgcatcgata tgaagcaggt tgaagcactg
     3481 gtggaagcgt ctaaagaaga agtgaaagca gctgccgcgc cagttactgg cccactggca
     3541 gatgatccga ttcaggaaac catcaccttt gacgacttcg ccaaagttga cctgcgcgtg
     3601 gcgctgattg aaaacgcaga gtttgttgaa ggttctgaca aactgctacg cctgacgctg
     3661 gatctcggcg gtgaaaaacg caatgtcttc tccggcattc gttctgctta cccggatccg
     3721 caggcactga ttggccgtca caccattatg gtggctaacc tggcaccacg taaaatgcgc
     3781 ttcggtatct ctgaaggcat ggtgatggct gccggtcctg gcgggaaaga tattttcctg
     3841 ctaagcccgg atgccggtgc taaaccgggt catcaggtga aataatcccc cttcaaggcg
     3901 ctgcatcgac agcgcctttt ctttataaat tcctaaagtt gttttcttgt ttgttcaacg
     3961 ctcttaattt ttttcattaa ctgctagcat ccgcttataa ctggatataa atacagggaa
     4021 cgcggatgaa gagttatatc tatcaggacg aaaaatcaca taaattctgg gcggtggagc
     4081 agcagggaaa cgagttgcat atcagctggg gaaaagttgg tacccaaggg caaagccagg
     4141 taaaaaattt tgctgatgtt gcagcagcgg aaaaagcgaa actgaagctt atcgcggaga
     4201 aaacgaaaaa aggctatgtg gaaaatgctt cagcaaacgt gcatatcccc cccattacca
     4261 aagccactcc tgaagttgaa acttcccctg agagtaaaaa ccaacgcccc tggctggctg
     4321 atgatgcggt cattcggcta actgacgata ttaatcgatt tgcttttcct catcgctctc
     4381 gccccagaga gatcaattat ctgcacaaag atggtcaaat atggcagctc atcgcgaata
     4441 acaccaaggc atatgatccc gccaacaatt accgttctta tccagaaaac tggcagcagg
     4501 cttttgctga gttacaaatg cgagttcagg gtaatcaaca gacagggagt gtgcaatctg
     4561 atgcagcatt gctctggagt ttctggaact cttactctgc agatgaactg atcgatgact
     4621 tagtcattcg ctgtggtctg gaaagtgccg ttgaaattgc ccttctcgct cttcaactta
     4681 gatataaacc agtaaaagcc gacgtcacca ccatcattcc ccctgatctc aaagcagaat
     4741 cgctaccgaa ctggcatcaa cgtctatgtc atcatctttc actcgcttca gaagatgagt
     4801 ggcaacgctg tgtagataag gtactcgcgg ctattccctc gctatcccca gcacggcaac
     4861 cttttgcggc gttactgatc cctgaacgcc cggatatcgc caatgctatg gcgctacatt
     4921 atgcagatca aaacgttccg gcgatgacct ggttaagtat gatggtaagc gatgatgttg
     4981 ccctggccac cctggaaaaa tatggtttcc caccgctata taatgatttt cgcaaatatc
     5041 tggctacgtt gctggcaaat aatggaatgc gtggtgtaag ccggatactt ctcaagctac
     5101 ctgtcgacta tcccgtaaaa tacacggacc ttttcactca catacacgcc aatgctgaag
     5161 atcttgtcaa atggctatgg aaaacgaatc acccagatgc gattcaaatt ctgatcctcg
     5221 gtgtaaatgg caagaaaaag cacctggaat acttaagcaa agcctgccaa aaacatcccg
     5281 ctgcggctat tgcggcttat gctactttgc tggcaataca tgaagataaa gagtggcgta
     5341 aagcgcttgt caaactgatt accgccacgc cagagttagt atgcgacgtc attccctggg
     5401 ttaatgctaa agcggcaggc atcttatctg aatgtcgtcc gcagccagta gctgaagaat
     5461 gtgaatatgc cactgtggat atgttgccgg agctgcttgt ttcgccgccc tggatgacca
     5521 aagagaaaaa gaaaaatacg cctgtgtttg atcttccagt gcttccagtt ccttctgttt
     5581 ctgatgtcac tccagagatt accaaaaaac tgacccgtac ttaccttgtc acccacttcc
     5641 agcaaatagc ccaacagcag gcgacaaaac aaacattgtt taccgacctg ccgccgatca
     5701 aaaaagcaag ctgggagaaa cacttaatac cgcttacacc ggagcaacaa atactgtggc
     5761 atcttggttt tgaaaaatgg cgggaaagcg gagaaaaaat atacgaaaaa attccggcgc
     5821 cacaaagcgc cgttgatgcc ctgctacgct tcgattttcc agcactcaat gctgaatttg
     5881 tgcaatatca taacaatgca tacaaaagct ggaatctcat tgccctctgt tacctgcctg
     5941 gtcaacaggc catttctttt cttaaccaga tagtcaaaga agataactac tctggtgagg
     6001 gaaatattct ggcaattttt ggtagcgccg ccatacctgc atttatggcc tgcctgcaag
     6061 gtgatccacg acgtctgtgc ttcttcccct ttttcctcgg tgtcagtgaa ctggccttac
     6121 cgatggcgca acaattacag aaaaaaatgt cttatgaaga tgcgcgaaac tggctaactg
     6181 attatccacg ccatgccgcc gccgggctac ttcctgtggc cctgggcaaa aaaggaaaag
     6241 atcgtgattg tgcccgccag gcgcttcgtc tgctggtgaa tttaaaccag cgagaaacga
     6301 ttgaggaaat cgcgcaagga tataatcaac ctgatgtttt agctgcgctg gcaacattgt
     6361 tcgatagcga cccactggaa gaatatcccg ctaaaatcgc cccactgtcc ggcttttatc
     6421 agttcacctt gtggcgcaga ccacggctta aaagtaacaa cctgcctctg tcagatgacg
     6481 ctatgcgcca cctcggcact atgctgagct ttcctcgcga cattaccgcc tacgctgggc
     6541 tggatatcat tagagaaatc ttcacccgcg aatcactggc tgaatttggc tgggatctgt
     6601 ataccgcctg gacagaagct ggcgcacccg caaaagaaaa ctgggcattt acttcgcttg
     6661 ggattttagg caatgacgac accgcgcgta aattaacccc gcttatccgc gcctggcctg
     6721 gggaatccca gcataaacgg gcggtgtccg ggctggatgt attagctgat attggcagcg
     6781 atgttgcgct aatgctgctt aatggcatcg cgcgaaaaat taaattcaaa gcattacagg
     6841 aacatgcccg cgagaaaatc aacatagttg ctgaaaaccg tgggctgact atggctgaac
     6901 tggaagaccg cctggctcca gatttagggc ttgatagcag tggctcgctg atactggatt
     6961 tcggcccccg caaattcacc gttggttttg acgaaacctt aaaacctgta gtgtgcgatg
     7021 caaacggcaa agtcctgaaa gatttaccta agccaaacca gagcgatgat aaaacactgg
     7081 caactgacgc agttaatctc ttcaaacagt tgaaaaaaga tgttcgtgct atcgccagcc
     7141 agcaaattac ccgtctggaa caggctatgt gccagcgcag acgctggacg gcagagcagt
     7201 tccgcctgtt tctggtggag catccgctgg tacgtcactt aacccgacgg ctgttatggg
     7261 gggtttataa cgatgaaaac gctcttatca cctgttttcg cgtggcagaa gacagcactt
     7321 acagcgatgc gcaggatgag ttattcacgc tgccagcagg aaacatcggt attccgcatg
     7381 tgctggaaat atcccctgaa tccgccgctg cattcaggca aatttacgct gattacgaac
     7441 tgcttccccc tttccaacag ctcgagcgag gtagctatca ccttgctgat aatgaacgta
     7501 atactcacga actgacacgc tggcaaggac ggctttgcca ggccggacgc attgtcgggc
     7561 tggaacgcag aggctggcaa cgtctggaag aaagcggcag cgtttatgcg atgcgcaaaa
     7621 caacccctca tggcgatctc gaacttgaaa cggaaccttt ttcattaatt tatggtgaaa
     7681 cggggtatgg cgaccagcta ccggttgaaa gcgtaaaaat cacctcgcca gatgatcgct
     7741 acggtaaaca atcctcactc actttctcca tgctcgacga catcaccgcc agcgagctga
     7801 ttaacgacat cgaatcactg tttgattaaa aggatttaac aatgaaaacc tatatttatc
     7861 aggacgaaaa atcacataaa ttctgggcgg tggagcagca ggaaaacgag ctgcatatta
     7921 cctggggtaa agtcggtagt aacgggcaaa gtcaggtaaa aagctttgct gatgctacgg
     7981 cggcggaaaa agcggaactg aagctgattg cggagaaggt gaagaagggg tatgtggagc
     8041 aagtagacgc taacgctcac tcacatgatg cctcagcaaa gaaagtcctg gtcacagagg
     8101 aaaaagcctc aataaatcgc caggcagcaa ctaatggttt accctggctg gcagacaacg
     8161 atcccattat tcttcccccg aatattgccc gccatgccct cagccaccgc ctttggccgg
     8221 gagagccagt aaaaaaacca accaaacccg agaaacaaaa tttaatttcc cgattggcaa
     8281 atcgtactta tcattattat aaaaatctga ctttcgatta cagctcatgc tctgatgaat
     8341 gggctgatgc gattagccag gcaatcagct taattaaggc ccaaaagact gagatacccg
     8401 cgcgtactat ggcggtactt acttttctgg aaatatgttt ttgtcagggc tggaataatg
     8461 aaacctcatc cgagttgctg gatgaaattg taagcacctt cggtatcgaa tacgcaactg
     8521 aaactgtcat cttctggtgg caaattgatt tcgattttga ctataaagat gaacttttga
     8581 ctttctttat taattatgca gatagcagca aggacagtta tagaaggaat aacgacaaat
     8641 ttagtttacg tctgcgtaaa catttatcac tagcagaaga agaagtatgg caaaattgca
     8701 ttgcaaaatt actcgttgcg ttaccagata tatcactctt tcgccaaccg ttaattgcca
     8761 ttttaatgcc agaaataccc gaagtggccc atgagatcgt acatcgttta caccaggtcg
     8821 cagatgttcc acaacttgaa atgttgaaac tggtagcgac agatccattc acgctcgaaa
     8881 tactggagaa ctatcaatac attgatgtat ttaacaccta tggtgccagt tggtcagcta
     8941 cggtattacg ggagcaaggt atagcggcac tggctcgcct ggctccctat gctgaagaag
     9001 ataattgcgg tgacgtactg aaatgtatta atcatccgtt ggccattacg gcactcatcg
     9061 aagccgccga tactaataaa cgctgttatg aacgactaat aaaaagtgcc gaaaattttc
     9121 cttctgccac gatagcggca ttagccgaag cattggtaaa aaaagatgat aaacgcaaac
     9181 atacgttgct ggtttcatta cttaatgcaa atcctttatt ggttgaaaaa ttagtcatcg
     9241 gattatcgtc gcaagcggca aatttattgc aacaatgtca gcaattgctg aatagtcgca
     9301 atgaagattt tgcaaactca gaaattctac caatggtact agtctcgccg ccctgggaaa
     9361 ctcaaaataa aaataacgac aacagtcctg tgggtaaata cccggcgaaa atcccccctt
     9421 taccgttgtt ttatcaacct ccattatgga ctcgcccgaa aatcaaaaat agcagcaagg
     9481 tactgccaga tagcgcactc cagcacctcg gtgaaatgct ccgcttccct caggaagagg
     9541 ccctgtatcc gggattattg caggtgaaag atgcctgtac cgccgactca ctggctgaat
     9601 ttgcctggga tctgtttacc gcctggttga ctgcgggcgc accgtcgaaa gagagttggg
     9661 cgttcactgc gttaggcgtt cttggtaacg atgacaccgc ccgcaaactg acgccattaa
     9721 tacgcgcctg gcctggtgaa tcccagcata aacgcgccac cgttgggttg gatattctcg
     9781 ccgctatcgg tagtgatatc gccctgatgc agcttaacgg tattgcccag aaactgaaat
     9841 tcaaagcatt acaggagcgg gcaaaagaaa aaattgccga cattgccgaa agccgcgaac
     9901 tcacggtggc agagcttgaa gatcggttag caccggatct cggtctggat gataacggtt
     9961 cgttgctgct ggatttcggc tcacgccagt tcaccgtcag ctttgatgaa accttaaaac
    10021 cgtttgtgcg tgatgtttct ggcagccgtc tgaaagacct gcccaaacca aacaaaagcg
    10081 atgatgaatc acaggcgaac gatgctgtta accgctacaa attgctgaaa aaagatgcgc
    10141 gtaccgtcgc cgcccagcag gtagcaaggc tggaatccgc catgtgcctg cgccgccgct
    10201 ggtcgccgga aaacttccag ctcttcctgg ttgagcatcc gttggttcgc cacttaaccc
    10261 gccgtctgat ttggggtgtt tatagcgccg aaaaccagct actggcttgc tttcgcgtag
    10321 cggaagataa cagctacagt accgctgacg atgatctttt caccctgccg gaaggcgata
    10381 tctctatcgg cattcctcac gttctggaaa tatcaccaac ggatgctgcc gcctttggtc
    10441 agctttttgc cgactacgaa ctgctaccac cgttccgtca gcttgaccgt aacagttacg
    10501 ccctgacaga agccgagcgc aacgccagcg aactgacccg ctgggcaggc agaaaatgcc
    10561 cgagcggtcg ggtcatgggg ctggcgaata aaggctggat aaagggcacc ccgcaggatg
    10621 gaggctggat cggctggatg atcaaacctc tgggccgctg gtcgttaatc atggaaatcg
    10681 atgaaggttt tgcggtaggc atgtcgccag ccgaactgag tgccgagcag ctcttaagca
    10741 agctgtggct atgggaaggc aaagcagaaa gctatggctg ggggagtaat tcaacacagg
    10801 aagcgcagct ctccgtactc gatgccatca ccgccagcga gctgattaac gatattgaag
    10861 ctctgtttga ataaggaaag tgcatggaca aggaattacc gtggctggcg gataacgccc
    10921 aagtggaact gaaatataaa aaaggcaaaa caccactcag ccatcgcaat tggccgggcg
    10981 aaccggtgcc tgttatcact gaaagcctca tccagacact gggtgatgaa ttgctacaaa
    11041 aagcagagaa gaaaaaaaac attgtctggc gttatgaaaa tttttcactg gagtggcagt
    11101 ccgccattac gcaggccatc aacttgatcg gcgaacacaa accctcagtc ccggcccgca
    11161 caatggcggc gctagcctgt atcgcgcaaa atgacagcca acagttgctc gacgaaatcg
    11221 tccaacaaga ggggctggaa tatgcgactg aggtggtgat tgcacgccag tttattgcac
    11281 ggtgttatga gagtgatcct ctggtagtca cattgcagta tcagaacgag gactatggct
    11341 atggttatcg ctcagaaacc tataacgaat tcgatctccg actgcgtaaa cacctctctc
    11401 tggcagagaa aagctgctgg cagcgttgcg ccgacaaact cattgccgca ctgccaggaa
    11461 taaccaaagt tcgccgtcct tttattgcgc tgatcctccc ggaaaagcct gaaatagcca
    11521 atgaactcgc cagtcttgaa agctcccggt caagtctcca ttcaaaggag tggttaaagg
    11581 tcgttgctac tgataatacg gcggtaaaaa aactcgaacg atactggggc ctggacgtat
    11641 ttagcgatcg cgaagccagc tacatgtcac aaaaaaaccg cttcggctac gcggcctgcg
    11701 cctccctttt gcgcgaacaa ggactggcag ccgttccgcg cctggcaatg tatgctcata
    11761 aagaagattg cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc
    11821 tgttagttgc tgataaaaac aaccccagcc tgcaacgtgt ggctaaatac agtaaaaact
    11881 tcccccatgc gacgctcgcc gcactggcag aactgctggc gttaaaagaa ccaccagccc
    11941 gccctggtta tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa gctcgagatg
    12001 aatactggcg tacgctgtta caaacgctga tggcatcgca gccacaactg gcagcagaag
    12061 tgatgccgtg gttaagtact caagccaggg cagtgctgaa gagttattta tcggcactgc
    12121 caaaaccggt tattgatggc accgataaca gcaatctgcc tgaaatcctc gtttcaccac
    12181 cgtggcgtag taagaaaaaa atgacagctc cacgtcttga tttggcaccg ctcgaattaa
    12241 ccccgcaagt ttactggcaa ccaggcgaac aagagaggct tgcctccact gagtctgccc
    12301 gttatttcag cacggaatct cttgcggaac gcatggaaca aaaaagtggg cgagttgtat
    12361 tacaggaact gggttttggg gatgatgtat ggctgtttct gaattatata ctccccggaa
    12421 aactggatgc tgcacgcaat tcactcattg ttcagtggca ttactaccag gggcgggttg
    12481 aagagatcct gaatggctgg aactccccgg aagcacaatt agcagaacag gcgctccgca
    12541 gcggtcacat agaagcgtta attaacatat gggaaaatga caactactca cgttatcgtc
    12601 cggaaaagag tgtctggaac ctgtatttat tggcacagtt gccgcgtgag atggctttga
    12661 ccttctggct gcgtatcaat gagaaaaaac atctgttcgc gggtgaggac tattttctca
    12721 gtatcctcgg gttggatgcg ctaccaggtc tgatgttggc tttttcacat cgtccaaaag
    12781 aaacatttcc gttaatttta aattttggcg caacagaact ggccctgccc gttgcccgcg
    12841 tctggcgacg tttcgcggtg cagcgtgatc tggctcgcca gtggatttta cattggccgg
    12901 aacatacggc tactgcactt atccctcttg tcttcaccaa atccagcgat aatagcgaag
    12961 ctgcattact tgccctgcgt ttactttacg aacagggaca tggtgaattg ctgcaaaccg
    13021 tggcaaaccg ctggcagcgt acagatgtat ggcctgccct ggagcattta cttaaacaga
    13081 gtccaataga gatttacccg gcacgcattc caaaagcccc tgatttctgg catccgcaaa
    13141 tgtggtccag gccgcgcctt atcactaata atcaaactgt taccgatgac gctctggaaa
    13201 ttatcggcga aatgctgcgc tttacccagg ggggacgttt ttatagcggg ctggaacaac
    13261 tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc ctgggatctc ttcactgcgt
    13321 ggcaacaagc tggtgccccc gcaaaagaca actgggcatt tctggcgtta agtctctttg
    13381 gtgacgaaag cacggcacgg gatctgacga cacagatcct cgcctggcca caagaaggca
    13441 aatctgcccg tgctgtcagc ggcctgaaca tccttaccct gatgaataat gatatggcgc
    13501 tgatacagct gcatcatata tcgcaacggg ctaaatcccg ccccttacgt gataacgcgg
    13561 cggaatttct tcaggtggtc gcagaaaatc gcgggctaag ccaggaagag ctagcggaca
    13621 gattagtccc aaccctgggc cttgatgatc cgcaggcgtt gagttttgat tttggtcccc
    13681 ggcattttac cgttcgcttc gatgaaaacc ttaacccggt tatctttgat cagcaaaacg
    13741 ttcgccagaa aagcgttccc cggttgcgcg ccgatgacga tcaactgaaa gcgcccgagg
    13801 cactggcccg actaaaaggg ctaaaaaaag atgccactca ggtgagcaaa aacctgctcc
    13861 cgcgtcttga aacggcccta cgtaccactc gacgctggtc gctggcagat tttcattctc
    13921 tgtttgttaa tcatcccttt acccgtctgg ttacccagcg attaatatgg ggggtttatc
    13981 cggcaaatga accgcgttgt ttactcaacg cctttcgtgt ggccgcagag ggggagttct
    14041 gcaatgcgca agatgagcca attgacctgc ctgcggacgc tctgattggc attgcccacc
    14101 tgttagaaat gacagtagaa atgcgcagtg aatttgcaca gctttttgcc gattacgaaa
    14161 ttatgccgcc ttttcgccag ttgtcgcggc gcacggtgct gctcacacct gacgagtcaa
    14221 ccagtaacag cctgactcgc tgggaaggta aatccgctac cgttgggcaa cttatgggaa
    14281 tgcgatacaa aggctgggag tcaggctatg aggacgcatt tgtctatgac ctgggtgagt
    14341 accggctggt ccttaagttt tcacccggtt ttaaccacta caatgttgat agcaaagcgc
    14401 taatgagctt ccgttctctt cgagtgtacc gtgacaataa atccgtcact tttgccgaac
    14461 ttgatgtgtt tgatttgagt gaggcgttaa gcgcacctga cgtcattttc cattaaaaca
    14521 cagcgggcag tgatgcaact gcccgttatc aacacgactt accagcgggg aaagcgatga
    14581 tcgtgcaaaa agagctagtt gctatttacg attatgaggt ccctgtacct gaagatccgt
    14641 tttccttcag acttgagatc cataaatgct ctgaattatt tacaggttcc gtctatcgac
    14701 tggagcgatt ccggctacat ccaacatttc atcaacgtga tcgagaagat gctgacccgc
    14761 taataaatga tgcgttgatt tatataagag atgagtgtat tgatgagcgg aaattacgag
    14821 gtgaatcacc tgaaactgta atagcaattt ttaatcgtga actacagaat atattcaacc
    14881 aagaaataga ataatatact ctagataatt aaagtcgcag cacatgcaac ttgaagtatg
    14941 acgagtatag ctttcttaaa gggggaaacg gtattccccc ttagatgcgt ttcaatttgc
    15001 ccgttacttc aacaaatcaa tcaaccgtaa cagagccact aataactcca gtacggcaat
    15061 gagcacggtt aacttttgtg tcagcgtgtc catccgctct ccttattaag gagcgcaaca
    15121 ccagcacctt acccttacac tgcctgtcga tactggttta cgtatgttgg tacgaaacgt
    15181 tgctctccgg atcagggcac cgcctgcggc accacccgtc cagcagccag gacttaaaat
    15241 tggtccgatc cggcacaacc tcccaacggg caggattata aatctcattc actgaattgc
    15301 aatggagtgt ggatatggtt ataatttatt cgttggtacg aaatgttgca caatcagcaa
    15361 gttagcgtca ctgtagtaaa taaaagaacc tgcctcacca gcaggttttt ttattttctg
    15421 cgatctactt tccagatatt ttttgccaat agcccaccat aaatattcac atcccggtca
    15481 aatggtgcgt tgaatataaa atcgatgaac ttataaataa acatgagtta tatgatgaat
    15541 taataaaaac agtaatcatt ttattaaagg gggaagcggt tttccccctt agatgcgttt
    15601 caatttgccc gttacttcaa caaatcaatc aaccgtaaca gagctactaa tagctccagt
    15661 acagcaatga gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttattaagga
    15721 gcgcaacacc agcaccttac ccttaaactg cctgtcgata ctggtttacg tatgttggac
    15781 cacgatgttg cactccggat cagggcaccg cctgcagcac cacccgtccg gtagccagga
    15841 cttgaaatag gttcgatccg gcgcaacgtc ccaacggtct ggattataaa tctcattatc
    15901 ttaattgtaa cggggtccag ccgtggttat aattcgttcg ttggaacacg atgttgcaca
    15961 ggctgtggtg ttaggcctga aaatagtaaa taaaagaacc tgcctcacca gcagtttttt
    16021 ttatttactg tgatctgctt tccagatatt tttcgctcaa acaactaatg cgccaaacat
    16081 ttattgcgcg taaaatatcg tttatttcat taatacattt cagggatgaa tatatgtcac
    16141 cgcagaataa tcatctgcag cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa
    16201 aattaaaaca aaatgatgcc gcaccttgcc cgcccggttg gcagttaagt ttgcctgcgg
    16261 cccgtgcttt tatccttggc gatagcgcgc aaaatattag ccgtaaagtg gtgattagtc
    16321 cctccgctgt cgaacgtatg ttagtcactc ttgccaccgg gcgcggtttg atgttggtgg
    16381 gggaacctgg caccgcaaaa tctctcctgt ctgaattact ggcaaccgct attagcggcg
    16441 atgccgggtt aaccatccag ggcggcgcat ctactactga agatcaaatc aagtacggct
    16501 ggaactacgc cctgcttatc aatcatggtc catcaacgga agcgctggtc cctgccccgc
    16561 tttaccaggg aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc
    16621 tggaagtgca agactgtctg ttaggtatgc tttctgacag ggtgatgacg gtgccggaac
    16681 tcactggtga agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca
    16741 atacccgcga tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg
    16801 aaactgtctt tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg
    16861 cgcgtttgct ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac
    16921 tggtccgcac cttccgcgat ctgcgtgcca acggcgaaaa gaaaacctca atggatacac
    16981 taacagcgat tatgtccacc gctgaagccg tgaacgtggc ccacgccgtg ggcgtccgcg
    17041 cctggttttt agcgaatcgc gcaggtgagc cagccgatct ggtggactgc attgccggaa
    17101 ccatcgtcaa agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacagcgtg
    17161 tcgccacaca taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt
    17221 gaggaaagat gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc
    17281 gcccggctgg tgaaatcgtt aatcgaaagc cagcggccac gatacgtgtt gattgaaggc
    17341 ccggctgatt ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct
    17401 atttacagtt attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca
    17461 tttgctgaat tttcgccgga gtggcaggcg ctacaagccg ctcgtcgtat tcaggcacaa
    17521 acttacttca tcgatttgcc ttgctgggca cagagtgaag aagaggacga ttcgcctgat
    17581 acgcaagagg aaagccagac cttgctgctg cgtgccaccc gcatggataa cagcgatacc
    17641 ctgtgggatc acttgttcga agatgaaagc cagcaaactg cattaccctc tgcactggcg
    17701 cactattttg cccaactgcg gggcgatttc ctcggcgatg cactcaatcg tcagcgcgaa
    17761 gcctttatgg ctcgctggat tgcatgggcg gtgcagcaaa ataatggcga cgtgttagtc
    17821 gtctgcggtg gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg cccgcaggac
    17881 attaacaagc cagaattgcc ctcgctggca gatgccgtta caggttgtta tctcacaccc
    17941 tacagtgaaa agcgccttga tgtgctggca ggataccttt caggaatgcc tgccccggtc
    18001 tggcaaagct ggtgctggca gtggggctta cagcaggcgg gagaacaact gctaaaaacg
    18061 gttcttaccc gtttgcgcca acaccacttg cctgcttcga ccgcggatat ggctgccgct
    18121 catctgcatg cgatggcgct ggcacagttg cgcggacata aactaccgtt acgcactgac
    18181 tggctggatg ccatagcagg ctcgctgatt aaagaagccc tgaacgcgcc gttgccgtgg
    18241 agctatcgcg gagttattca tcccgacacc gatccgattc tgctaacgtt gatagacaca
    18301 ttagcgggtg acggattcgg taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa
    18361 gatgtcacct gcgaactgga acgtaccgca atctctcttc cggcggagct taccttaaat
    18421 cgctttaacc ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag
    18481 atcccaggga ttgtacgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa
    18541 cgctggaaat taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt
    18601 ggtgccacac tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt
    18661 ggaatcggca gtatcaccac atgtcttagc caggcggcgt tagcgggtct ggcgtccttc
    18721 agtcaacaat tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa
    18781 atggggcagg cgctggaagt cctttatgcc ttatggcggt tagatgaaat cagcggtatg
    18841 caaggcgcgc agatattgca gacgacgtta tgtgcggcta tcgatcgcac gctgtggctg
    18901 tgtgaatccg acggcaaacc ggatgaaaag gagtttcacg ctcacctgca tagctggcag
    18961 gcgctttgcc atatcctgcg cgatctacat agcggcgtta atttacccgg cgtttctctt
    19021 tctgcggcgg tggccttact ggaacgccgc agtcaggcaa ttcatgtccc ggcgctggat
    19081 cgcggcgcgg ctcttggcgc actgatgcgt ctggaacatc ccaacgccag tgccgaagcg
    19141 gcgctgacaa tgctggcgca gttatccccg gcacaatccg gcgaggcgct gcacggtttg
    19201 ctggcgctcg cccgtcatca actggcctgc cagccggcat ttatcgccgg tttcagcagt
    19261 catttaaatc aactgaatga cgacgatttt attaacgccc tgcccgattt acgcgcggcg
    19321 atggcctggc taccaccacg agaacgcggg acgctggcgc atcaggtgct tgagcattat
    19381 caactggcgc aacttcccgt ttcggcgctg caaatgccgt tgcattgtcc accacaagcc
    19441 attgcacatc atcaacaact cgaacagcag gcactggcat cgctgcaaaa ctggggagtt
    19501 ttccatgtct gaactgaacg atcttctgac caaccgtgag ctacaacgct ggcgattaat
    19561 tcttggcgaa gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga
    19621 ccacgcgctg gagtggctgt atgggcgcga tcctgaacgg ctacagcgtg gtgaacgctc
    19681 cggtggatta ggtggctcaa atctcaccac ccctgagtgg atcaacagta ttcacacgct
    19741 gtttccgcaa caggtgattg agcggctgga aagcgatgcc gtactgcgct atggcattga
    19801 agatgtggtg acgaatctcg acgtgctgga acgtatgcag ccttctgaaa gcctgctacg
    19861 cgccgttttg cacaccaaac atctgatgaa ccccgaagta ctggctgccg cccgccggat
    19921 agtgcgccag gttgttgaag aaattatggc tcgactggca aaggaagttc gtcaggcttt
    19981 ttctggtgtc cgcgatcgcc gtcgccgctc atctattcca ctggcgcgaa actttgattt
    20041 caaaagtact ctacgcgcca acctgcaaca ctggcaccca caacacagca agttgtatat
    20101 cgaatccccc cgctttaaca gccgtattaa gcgccacagt gaacaatggc aactggtctt
    20161 actggttgat caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc
    20221 ctgtttgtgg cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt
    20281 taatctcacg gcagacgttg ccgatccggt agagttatta atgaaagtac agttgggcgg
    20341 cgggaccagt atcgccagtg ccgtggagta tgggcggcaa cttattgaac aaccagcaaa
    20401 aagcgtcatt atcctcgtga gcgattttta cgaagggggt tcatcatcat tgctgacgca
    20461 tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg ggactggcag cgctcgatag
    20521 cacagcaacg ccttgctatg accgcgatac ggcccaggcg ctggttaatg tcggcgcaca
    20581 aatagccgcc atgacgccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg
    20641 aattcactac gtccggaatt attagaactg acaccgcagg cactgacggc gttaagcaat
    20701 gccggttttg ttaagcgcag ccttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc
    20761 catgagaacg gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac
    20821 ggtcaggcac tgaaagaggc tcagtgcagc tgcggggcca gcggcatgtg ccgtcatcgc
    20881 gtgatgctgg tgttaagtta tcaacgactt tgtgctaccg cccagcctac gggaaaagaa
    20941 gaggaatggg atccggcaat ctggctggaa gaactggcta cccttcccga tgccacccgc
    21001 aaacgcgcac aggcgctggt cgctaaaggc atcaccattg agttgttctg tacgccgggc
    21061 gaaattccct ctgcccgctt accgatgagc gatgtgcgtt tttattcccg tagcagcatt
    21121 cgtttcgccc gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggcagta
    21181 caggcctttg tccaggcaaa agcgcagcaa gcggaattga ctcatttaat ctggcagatg
    21241 cgcagcgaac acgtcacatc atctgacgac ccgtttgcca gcgaagaagg caaaacgtgt
    21301 cgtcaatatg ttcagcaact aagccaggca ttatggctgg gcggcatcag ccagccgctt
    21361 attcactacg aggccgcttt cagccgcgcg cagcaagcag cggaacgctg caactggcga
    21421 tgggtgagtg aatcgctacg gcaactgcgc gccagcgttg atgccttcca cgcccgcgcc
    21481 agccactatc atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat
    21541 tgcgcacaag agatggccca gcgcgacagt gttggtgaag ttcctcctgt gccgtggcgc
    21601 acggtcgttg gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct
    21661 ttaggtatgc gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat
    21721 cccgacaccg gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaaaac
    21781 tcaccggcag ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa
    21841 attgtttcac aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac
    21901 cgcttaagca gcgttgtgcc gctgtcgcct gatgcctggc gaatgttgag cgcgccgtta
    21961 cgccagccgg gcattgtggc tttgcgggaa tatttacgcc agcgtccccc ctcctgcata
    22021 cggcctctta atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc
    22081 ggttgggata gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat
    22141 aatctgctga cgttatcact gccagcctca gccagcgcgc cttatgccgt tgaacgcatg
    22201 gcggcgcttt tgcaacaaac agacgacccc gtgtgtctgg tttctggctt tgtcagtttt
    22261 gttgatgggc aattgacact ggaaccacag gtaatgatga caaagactcg tgcctgggcg
    22321 ctggacgcag aaactgcgcc tgtggtggca tcactacctt ctgccggcgt tctgcctgtg
    22381 ccgtctactg ctcatcagtt gctgatgcgc tgccaggcat tacttattca actgctccat
    22441 aacggctggc gctatcagga acagagtgct attggtcagg cagagttgct ggcgaatgac
    22501 ctcaccgcgg tgggttttta tcggctggca catatgttgg gacaatttcg taatacagaa
    22561 agcgaggcac gggtagaagc tatgaataac ggtgttttgc tgtgcgaaca attattcccc
    22621 atgcttcagc aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga
    22681 ctgctcacgc cacggtatta ggcatatcct atatagcccc tggtgagagt caccagttcc
    22741 ttgattaaat aaaatggagt tttacatgaa ggctttcaat aagctgtttt cccttgttgt
    22801 tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt
    22861 cagcgccaat ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt
    22921 gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc attggtgcaa ccaccaaaga
    22981 agatgctgcc aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga
    23041 agaaaaatta acctataccg atacctacgc gcaggaaaac gtgactatcg atatggaaaa
    23101 agtggatttt aaagccctgc agggtatttc aggaatcaac gtttctgctg aagatgccaa
    23161 aaaaggtatc actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaagaagt
    23221 gaaataatct gtcggcggcc atgtttcgca tggccgccat acccgtctta gcttttcttc
    23281 acatgctggc gcgctgccag tccacgcaga aaataacgta aaaattgatc gccgcattcg
    23341 cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat cgaaacgcgg
    23401 aactgctgtt cggtgaggat cgccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt
    23461 aattttttca gcacgatgtt gttattaatg cgacgttccg gctccagtgc cggagcagac
    23521 tcatccttgc cgcgtttttc ataaatcagg ccattgagga atgacgacaa aacaatgtcc
    23581 ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg
    23641 gtggcttcga cattacccag cgccagtata cgcaccaggt cattattatt ggctttcaaa
    23701 atgtagcgca cgctgcgcag aatatcgtta cttagcatga ggccttcagg tgttgatgag
    23761 gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc ttttcagata
    23821 acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct ggccgttatc
    23881 ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat ggcagcgcag
    23941 tagtggtgta cgactttcca gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc
    24001 gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg
    24061 cagcaaataa atccgactat gcccagtaca agggataaat ttcagcgcct gttgattttc
    24121 tggtaacagc gaaacatcct gcttgctgcg ttcctgccgc aaacgcgcca gcgttttctc
    24181 cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcgtgttctt caaaggcttt
    24241 aatggcgtat tcgtcaaacg cggtgagaaa aacaatatac gggcgatgtt ccgggtcaag
    24301 catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc
    24361 cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact caccaacgat
    24421 ttcaatatcg ctctgctcct gcaaaaatac gcgcaggttc tcccgtgcta acggttcatc
    24481 atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg
    24541 gtgtaactat caggctcaca ggcgacgctt atcccatagt catcgccaaa ccgttcacgt
    24601 aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa
    24661 ccggcattgt cttcgatctc cagcattaaa tgttgcccct cacgtcgggc gctgattgcc
    24721 actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt
    24781 tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc
    24841 tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcattcac atgttcaatt
    24901 tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa
    24961 agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg
    25021 ttaagcgcat taaacaaaaa atgggggttc acctgggcgt gaagcagttt gatctctgac
    25081 tgggtgagca tcgctttttg ccgctcatat tgcccggcaa ggatctgcgc cgaaagtagt
    25141 tgcgcaatcc cctcacccaa cgtgcggttg attgaactga ataaacggtt tttggcttca
    25201 tacaatttga tggtgcccat cacccgctga ttttcaccac gtaacggaat taccagcgtc
    25261 gaccccagtt tgcattgcgg atgcaaagag caacggtaag gtacttcgtt gccatcagcg
    25321 tagaccactt caccggtttc aatcgctttt aaggtataag tcgaagaaat cggtttgccg
    25381 ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc
    25441 gcgactgcac caatatccag ctcctgatac agcacctgtg ccactttcat gctgttcact
    25501 tcgttaaacc cctggcgcaa aatgccttcc gtcgaggccg ccactttcag cgcagtggca
    25561 gaaaaagctg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac
    25621 agcgccgcgc cgacggtatt ggtgaccatc attggcgcag caatattact caccagacgc
    25681 accgcatctt cataaggtcg ggcgatcgcc aggatgatca acatttgcac catttcagcg
    25741 acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg
    25801 atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggtcga gatcatgcaa
    25861 cttagcgcgg tcatgccccc catcgaatat cgatgtaacc cgccggtcag gccaaccagc
    25921 ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg
    25981 gcaatagaat cgtcaatgtg caacccaaac caggtgccca tgatgcagaa gatggaaaag
    26041 acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa cggtatgaat
    26101 aatggcgttt tactcattaa ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc
    26161 agcaacacca gattaaaatc gtacataccc gcaaaccaca cttcccttta aaacgcgtaa
    26221 catacattgc ctgcgtttaa ctttctttga actcttgcag aaaaatgaga attcgtgagt
    26281 acgatcactc aaaatcgcct ggcaaaaata aaatcaccct ataaatgcac aaaaaacggg
    26341 caaaactacc tggttcgcaa aactgcgtct aaagttaaac cgggacctcg cgagcaaggg
    26401 tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata ttaaccctgt
    26461 cacgttacgc gcgtggcaga ggcgttacgg attgctgaaa ccgcaacgga cagacggcgg
    26521 tcatcggctg ttcaacgatg ccgatattga ccggatccgc gagatcaaac gctggatcga
    26581 caacggcgtg caggtcagca aagttaaaat gctgctcagt aatgaaaatg ttgatgtgca
    26641 gaacggctgg cgcgatcagc aagaaacatt actgacctac ctgcaaagcg gcaatctgca
    26701 tagcctgcga acgtggatca aagagcgcgg tcaggattac cccgcccaga cgctcaccac
    26761 acatctgttt attcctctgc gccgacggct tcagtgccaa caaccgactc tccaggcgct
    26821 gctggcgatc ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg cttcggcacg
    26881 taaaaaacag ggtaaagatg cgctggtggt tggctggaat attcacgata ccacccgtct
    26941 gtggctggag ggctggattg ccagtcaaca aggctggcgc attgatgtcc tcgcccactc
    27001 actcaatcaa ctacgccctg aactgttcga aggccgtaca ttgctggtgt ggtgcggtga
    27061 aaatcgaacc tccgcccaac agcagcaact caccagttgg caagaacaag gctatgatat
    27121 tttcccactc ggcatttaat gattcgttaa caaatgcgct ttactgtaca atcctttcgt
    27181 taacataagg agtgcattat gcgcatagct aaaattgggg tcatcgccct gttcctgttt
    27241 atggcgttag gcggaattgg tggcgtcatg ctcgcaggtt atacctttat tttgcgtgct
    27301 ggctaagcgc ctgcaccagc ctttcaaaca ggcggtctgc gatgatcgcc gccagtgcca
    27361 ccagtaacgc cccctggatc acatacgcgg tattaaatcc gctaagcccg atgatgatgg
    27421 gcgtacccag cgtgctggcc cctaccgttg aggctatcgt cgccgtacca atgttgataa
    27481 tcaccgaagt tcgcacgccc gccagaatca ccggagccgc cagcggtagc tcgaccttac
    27541 gcagtcgctg accacgactc attcccatac ctttcgcaac ttctgtcacg ctggcatcaa
    27601 tcgctcccag cccggcaagt gtcgcctgca ggacgggcag cacaccgtaa aggatcaagg
    27661 cgataatcgc tggttgcaga ccaaacccga tcaccggaac ggcgatcgcc agcaccgcga
    27721 cgggcggaaa agtctgtcca acggcggcaa tagtttccac cagtgggcga aattccgcgc
    27781 cccacgggcg agtgacagca attccggcac cagtaccaat gatcaccgca aacaaactcg
    27841 aaattcccac cagccagaaa tgagccagtg ccagagctgc aaaactttct tgctgataaa
    27901 cgggtcgtgg cagttgtggg aacaaggcag caaacagcgg ctggctgtaa ggcagccaga
    27961 aaatcagtgc cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac atcttcatac
    28021 gcttacgcct ccaccagcag atcctgaaaa tgcagcgtgc cgcaaggctg gccctgcgtg
    28081 ttcaccaccg gcagcacctc gcatccccgc gcgacaaaca gagagagcgc atcgcgtagc
    28141 gtcatctctt ctgccagtgc ctcaccttct gctcgttctt cgcgacgcac gtaatccacc
    28201 acactacgta acgaaagcag gcgcacaccc agttcactac gtccaaaaaa ctggcggaca
    28261 aaatcattcg ccggacgagt cagcatcgtc agcggattcc cctgctgcac cacttcaccg
    28321 tgatccatca ataccagatg ttctgccagc cgtagcgcct catcaatatc atgagtgacc
    28381 agcacaatag tgcgccccag caaacggtga atgcgcgtca tctcttgttg caacgcgccg
    28441 cgcgttaccg ggtccagtgc gccaaaaggt tcatccatca gtaagacttg cggatcggca
    28501 gccagtgcgc gcgccactcc cacacgttgc tgctgaccac cggaaagctg atgtggataa
    28561 cgctcacgta aatttgactc cagccccagt agcgccatta attcgtcgat acgatcgtca
    28621 atcctcgccc gcgaccattt ttgtaattgc ggcacggtgg caatgttttg tgccacgctc
    28681 cagtggggaa acaggccaat agattgaatg gcatagccca tacggcggcg caactccagt
    28741 actggcagcg agcgaatttc ttctccggca aagcggatct ctccgctgtc atgttccacc
    28801 aggcggttaa tcattttcag ggtggtggat ttgccggaac cagatgtgcc aatcagcacc
    28861 gaaaaactcc cttcctgaaa attgagattg agatcgttaa cggctttttg tgcgccgaac
    28921 agtttgctga catggctaaa ttcaatcatt acgtttcacc ttcaacagtg cgataagtaa
    28981 atcgaacagc gcatcgatca gcaccgccag aacaattacc gggatcaccc ccagcaacac
    29041 taaatcaatg gcgctgctta gcagcccctg gaaaaccagc gcaccaaaac cgcctgcgcc
    29101 gattaacgcc gcaatcaccg ccatgcctac agtttgcacc atcaccaccc gcaggctgcg
    29161 cagaaatacc ggtaacgcca gcggtaactg aacatgcagg aatcgctgcg ccccgctcat
    29221 ccccatcgct ctggcgctct ccagcacatc gcgcgggatc tggttcaagc ctactaccac
    29281 gccgcgcacc agcggcagca aggcatagag caccagcgca atcagtgcgg gtgtcattcc
    29341 ggttcctgct atgccgaact tccccagcca cggaaaggcc gtaaccagtc cggcaagcgg
    29401 cgcaatcagc aggccaaaga gcgccaccga aggcacggtc tgaatgacat tgagcagaga
    29461 aaaaattgcc ccctgccgcg cagtggaaaa atagcaccag atgcccaacg gcacaccaat
    29521 cactaacgca ggcagcaccg caccaaacag caacgtcaga tgttgtgcca gcgcgtcgtc
    29581 aaacacatcc tgacggttgg cgtattcttt cattagtgag agatcgttaa gcgtgccgga
    29641 gtacagcaac cacagcggaa taatggcaat ctgcatatgc aacaaccagc gccacagcgg
    29701 atgcgtggag attcggcgga tggcatcgct acaggccagc aatgccagcg ccgcagccag
    29761 ccagaaacca ctgccgaggc tggtacgcgc cagcgcactg ccattttgcg ccagttgggt
    29821 cgccgccttt cctgctcccc acaccagcaa tacgaagacg aattgcgcca gaatgagtgc
    29881 aaaaatgctg ccttttttag cgggaataaa acaggctgtc agccaggcgc aaccaacgcc
    29941 caccagcatc caaatcgttt gcggccacag ctgccagaga tgtcgccctt cacccgagac
    30001 taaacgatta ggcgcgtaac tgataaacgg cagcgccgct gcgattgccg tcagcaacag
    30061 cagcagcgcc agaacaggat tgatacgtag ataagtcacg ggaaattact tcgtccaccc
    30121 tttttgtttc aggtagtcgg cagccacttt tttggcatcc agtccttcaa ctgcaatgct
    30181 ggcattcagt tgctgcaatg ttttttcatc gaggctggcg aagactggct gtagccactg
    30241 tgccatttgc ggatactcct tcaacaccga ttcacgcacc actggtgcag gcgcgtagat
    30301 aggttgcacg ccttgcggat cgcttaaggt ttgcagcccc agcgccgcga ccggaccgtc
    30361 agtgccgtaa gccattgcgg cattaacgcc agaagtttgt tgggcagcgg ctttaatcgt
    30421 taccgccgtg tcgccgccag ccagtgacag taactgatcc tgatcgagtt taaagtcgta
    30481 ggctttttca aacgcgggta acgcatcggc gcgttcgata aactctgccg aggctgccag
    30541 tttgaaggtg ccgccctctt tcagataacg accgagatcg gcaagcgaag tgagtttgtt
    30601 tttctctgcc acatcctgac gcacggcgat ggtccaggtg ttattcgccg gtgcaggcgt
    30661 cagccagatt aacttgtttt gctctgcatc gagttttttg actttctcgt aaccttgctg
    30721 cgcgtttttc catgccgcat cgttttcatc tttaaagaag aaagcgccat tgccggtata
    30781 ttccggatag atatccagtt caccggaagt aatcgccccg cgcaccacag gagtcgttcc
    30841 gagttgcact ttattcaccg tcggtacgcc gtggctttcg agtacttgca aaatgatatt
    30901 gccgagcagc gcgccttcgg tatcgatttt tgaaccgact ttaacgggag aagctgcctg
    30961 tagcggcagg ctcacggctg ccaacataac cagtgaacct gcccagacct ttgagagtgg
    31021 catgatgctt tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt
    31081 gataatgaca gagttagcct gagcggacgg attcagttgc agaatcagat aaatacttaa
    31141 gaggcatatt cggtccggcg tttcctgccg gatgcggcgc gagcgcctta tccggcctac
    31201 aaagggcgaa aattcagcaa gttaagatat gaacatcagg caaagggcgc aaacgtcgcg
    31261 cccttactaa agcattacag caactcaaac tcgcctttct taacgcgtgc ggaatcagtg
    31321 ccgataaaga cattgaactt gccaggctcg gcgtcatatt tcatctgttg attccagaac
    31381 ttcagcgcct cgatatcaat cgggaagcta acggtctgag tttcgcccgg tttcagggtg
    31441 attttctcaa agcctttcag ctgtttcacc ggacgactca tggaagccgt cacatcctgc
    31501 aagtacatct gcactaccgt ggccccttcg cgtttaccgg tgttcgtcac ctgcacgctg
    31561 gccgtcactt tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc agagacggtg
    31621 aaagtggtat agctcagacc atagccgaac ggataaagcg ccccgttagc ttcatcaaaa
    31681 taacgcgaag tgtatttgtt cggcttgtcg gcattatacg gacgaccggt gttcagatgg
    31741 ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acatcggcag cttgccggac
    31801 gggttgtaat cgccaaacaa tacatcggca attgcattac cgccttcagt ccccgcaaac
    31861 caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgcccg
    31921 ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt
    31981 tggctttgcg gaatagtaat atcggtacgg ctggaagctt cgtgcgccat cccctgagct
    32041 tcaccgacca cagccaccac cacatcagat tgtttcgccg tctgcaccgc ttcatcaatc
    32101 atctcttgcg gcgaacgcgg atcgactttg accgcttctt catactgatt caggaaatcg
    32161 ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca
    32221 ccgacggcat ttttaatccc ggtcagcacg gtcacggatt gatcggcaac accggctgcg
    32281 gaccagctgc ccatcacgtc acgcttgctg tctgccagcg ggccaaccac cgcaatggtt
    32341 gcggattttt tcagcggtaa cgtttcgaga cggtttttca gcaacaccag gctttcgcgc
    32401 gccacttcac gcgcttcttt gcggtgcagg cggctttcgg cattggtatc caccgggtca
    32461 gactctttcg gaccgagatg gctgtatggg tcgttaaaca accccatatc atatttaacg
    32521 ttcagtacat ggcgggcagc atcgtccagc tctgccatcg tcactttgcc ggatttgatc
    32581 aacccaggca gatacttcga gtagtactcg tcgctcatgc tcatgttgat tccggatttc
    32641 agcgccacgc gcaccgcatc ttccgggtct gccgccgtgc catgtttaat cagttctttg
    32701 attgcaccgt gatcggaaac ggtgatgcct ttaaaacccc actgatcgcg cagaacatct
    32761 ttcagcagcc aggagtcgga ggtggctggc gtgccgttga gtgagttcag cgccaccatt
    32821 accgcaccgc tgcctgcgtc cagccccgct ttgtacggcg gcatataatc attaaacagg
    32881 cgctgcgaac tcatatcgac ggtgttgtat tctttaccgc cttctaccgc gccgtaagcg
    32941 gcaaagtgtt tgacgctggt catcaccgag tagcgatctg ccgggctttt accctgcatc
    33001 gcttccacca tagttttacc cattattgaa gtgagatacg tatcttcgcc aaaaccttcg
    33061 gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc
    33121 aggccatcat ctgccgcttc ataagcagag acacgtccga ccgtttttac tgcatcgagg
    33181 ttaaaagacg aggccagacc gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg
    33241 tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg
    33301 gcgcggatat cctgacgggt tacggtgttg aaaatcgctc caacctgacc gtctttgatc
    33361 atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg cagctgacca
    33421 attttctcat caactgtcat tttcttaagc agttcggtga cgaacgcatc ccgcgcttca
    33481 ggcgttaatg gatggttacc gaataaatca tccgccagtg ctggctgcag ggccagactc
    33541 accgcgattc ctactgaaca tagccatttc atatggattt ttcttccttt gttgcccgac
    33601 gtggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa
    33661 gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg
    33721 cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga
    33781 caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc
    33841 tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg
    33901 acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg ctgaaagcct
    33961 gcgtcaccgc cgacaaaatt attctgatgc aggctgccaa tacaggcctg accgaaggat
    34021 cgacgccaaa cggtaacgat tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg
    34081 acaagctgca cgttctcggc aagggcgaac aggtgctggc ctatccgggc accacgctct
    34141 attcgctgga aaaagccctc aaaccgctgg gacgcgaacc gcactcggtg attggatcat
    34201 cgtgtatagg cgcatcggtc atcggcggta tctgtaataa ctctggtggt tcgctggtgc
    34261 agcgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa gatggcaaac
    34321 tgacgctggt gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca
    34381 agctggatga cgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg
    34441 attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg
    34501 ccgatccgga tcggttattt gaatcttctg gttgcgccgg gaagctggcc gtctttgcag
    34561 tacgtcttga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc
    34621 agccggaagt gctgaccgaa atccgccgtc atattctggc taacttcgaa aatctgccgg
    34681 ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca
    34741 ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg
    34801 gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt actgaccgtg
    34861 cgatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc
    34921 gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtt ggtgaagcca
    34981 aatcgtggct ggtggattat ttcaaacagg ccgagggcga tttctttgtc tgtacgccgg
    35041 aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc
    35101 aggcggtgca ttccgatgaa gtcgaagaca ttctggcgct ggatatcgct ctgcggcgta
    35161 acgacaccga gtggtatgag catttaccgc cggagatcga cagccagctg gtgcacaagc
    35221 tctattacgg ccattttatg tgctatgtct tccatcagga ttacatcgtt aaaaaaggcg
    35281 tggatgtgca tgcgttgaaa gaacagatgc tggagctgct acagcagcgc ggcgcgcaat
    35341 accctgccga acataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagtttt
    35401 atcgcgagaa cgatccgacc aacagtatga atccggggat cggtaaaacc agtaagcgga
    35461 aaaactggca ggaagtggag taaaaatgac ggatggcaga gtatcgccat ccctaattca
    35521 cttaatcgtt ctgtgccgtc tgccccgccg ccgccatttg ggcggctttt tgttttttat
    35581 agctcaacgc tgctgccgga acaggcatca ctttaccggt ttcaatccag gtacgcaggc
    35641 ggctggcatc ggcaaaatgg gtatatttgc caaacgcatc catcactacc agcgccaccg
    35701 gtttattatt gataaccgta cgcatcacca gacaatggcc cgccgcattg gtaaagccgg
    35761 ttttggttag ctggatattc cagttatcgc gatacaccag atgattagta ttgcggaacg
    35821 gcagcgtata cgtcgggtta gagaaggttg ccatatcttc ccgggtggta cttaactgcc
    35881 cgatcaacgg atattgtttg ctggcaatga gcagtttggt taagtcacgg gcggttgaaa
    35941 cgttatgcac cgatagtccg gtcggttcaa caaagcgcgt gttgttcatt ccgagcgatt
    36001 tcgctttcgc attcatcgcc ttaataaagg ctttgtaacc accgggataa tggtgcgcaa
    36061 ggcttgccgc cgcgcggttt tctgaagaca tcaacgccag caacagcata tctttacggc
    36121 tgatttcgct gttcagtcgt acgcgcgaat agaccccttt catctccggc gtctggctga
    36181 tatccacttt tagtttttca tccagcggca gtcgcgcatc cagcacaacc atcgcggtca
    36241 ttaatttgct gatagacgca atcggacgca ccagatccgg gtggttcgaa tagatcactt
    36301 tgttggtatt cagatcaaca atcatcgcgc taccggaggc aatttccggt tgtgaagcgg
    36361 tggtagcggc tgccgttttc gcaacggcct gcggtgccaa aggcacagcc agcatcaggg
    36421 ccaggctaaa taaagaaact cgaaatttcg gcatgatgag cattcagata gtggttcacg
    36481 cgcacgggtt gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc catcataacg
    36541 agcaaaaagt gcgatcgtca aaggagaatc gtgaggaaat gctgcattgc tgacatttac
    36601 gccagcaatg caacgtcaaa gaactttcta gaacaaacga taaccgtagc cccatagtat
    36661 aacggtaagg gcgagcagca cttccagtac cagcacgcca atcgccagcg tcgaactgga
    36721 gaagctaagg ccttcctctt tgttgatatt caggaagctc ggaataccaa ggtaaagcag
    36781 gtaaccggtg taaaacagtg ccaccgtgcc gaccagcgca cacaaccaga ccagcggata
    36841 aagcgccacc agaccgctta aaaacagcgg agttgcaacg tagcccgcga agaccatgca
    36901 gtgcgcaagt gacggacgct gcgggtaatt gcgcgccatc caccagatga cccgccccat
    36961 caccgcaacc ccagccagca taacgccgta aaacaagaca gccagcgcca gtccggtaaa
    37021 ccaggataac ttcaggatag tgccatcgcc aaaattccag ccaatctgtg tagtgccaat
    37081 gaaggcgcaa atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga
    37141 aatcgtttcg ttttcgcgat taatcacctg catttcacga tcgggatggg aaaacagtcc
    37201 ccagacatgg ctcataccgc ccccttgttg tgagttcatg aacctgacag ttcaagtata
    37261 agtcagcttg tgattatttt ttgttcgctc atgtaatttg caccgtctga tgtacgtcct
    37321 ttttcagcgt gaatgatttc cagggtgtat gcttacaggc aaccaaaggg agacagactg
    37381 gcctatggat ctcaatacac tcatctcaca atatggttat gccgcgctgg tgatcggtag
    37441 cctggcggaa ggtgaaaccg tgactttgct gggaggcgtt gcggcgcatc aggggctatt
    37501 aaagttcccg ctggtggtac tttctgtggc gcttggcggc atgattggcg accaggtgct
    37561 ttatctgtgc gggcggcggt ttggcggcaa gctgttacgc cgtttctcga aacatcagga
    37621 taaaattgag cgggcgcaga aaattatcca acgccatccg tatctgtttg tcattggtac
    37681 gcgctttatg tatggctttc gggtgattgg cccgacgctg attggtgcca gccagctgcc
    37741 gccgaaaatc tttctgccgc tgaatattct cggcgcattt gcctgggcgt tgatttttac
    37801 cactatcggt tacgctggtg gtcaggtgat tgcgccgtgg ttgcacaatc tcgatcagca
    37861 tttgaagcac tgggtctggt tgattctggt cgtggttctg gtggtaggcg tgcgctggtg
    37921 gctgaaacgg cgcgggaaga aaaagccgga taatcaggcg taaaaccatt gccctggata
    37981 aggcgttcgc gccgcatccg acatccgggg caccatgcct gatgcgacgc ttgtgcgtct
    38041 tatcatgcct accaaaattt tacccattga aataaccacg cacctcactg cgctattctg
    38101 ggttgaactg cggattcgcc aacataaagc cgccatccac tatcaacgac tgcccggtgg
    38161 tgtaatttgc gccctctgag cacagccacg ctaccaggct ggcaatctca tgcgttgcgc
    38221 caaaacgccg cagaggaatc gaaggctccg cgtcgggctt cacgtcgctg tcatccatgc
    38281 cattcattgg cgtggcgatc gccccaggcg cgactgcgtt caccaaaatc ttatgtctga
    38341 ccagctccag cgccattgct ttggttaacc caccgagcgc atgtttagcg gctgtgtagg
    38401 cgctggcatc cggcagcggc gtatgttcat gtaccgacgt aatgttgatg atgcgaccgc
    38461 cctgcccttg tttcaccatc tggcgggccg caatttgtga gcataaaaat gcaccatcga
    38521 catcaacggt aaaaatcttg cgccactcat cgaaagccat atcaagaaac ggcgctttgg
    38581 tcattgcgcc cgcattattc accagcacat caatacgccc cagccgttga atgagtttct
    38641 ccaacgcctg cgcaccttct ggcagtttac cgagatccag ctgcacaatc tccgcacgta
    38701 cgccgtggct aactacctca cgcgcggtat cttttgcccc ttcttcatct gagtgccagg
    38761 taatgccaat atcaaaccct tgctgcgcca gtaataacgc gcactctttg ccgatccccg
    38821 aatcggaggc ggtaataatc gctacctgtg ccatcgagtt ctccacttaa cgctgaataa
    38881 acgttaagta tagaagtcac atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg
    38941 agttgaatgg aggcatccag ccactgccct tgcaataaca ggccattggc ccgctcacgc
    39001 agcgcgggga ttctggcttc gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgg
    39061 gcctgcgcaa gacctaccac acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt
    39121 ttctccgcca gtgtctgaac ctgactggct gcccgcgcca cgtcattcac cgcttcaacc
    39181 accgctttgt tgtagctggc gatagacaag ttgctttcgg cttttgcgat atcgagattg
    39241 gcgttaagac gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc
    39301 tgcgcggaat gacggaacag ctcgcttaag tgcaacgcat cctgttgcag gaaggccatc
    39361 aggttgatgt caggataaaa tgccgctttt gccgcatcaa tggtgcttag cgatgactca
    39421 acgtaccagt gcgccgcctg caaatctgcc cgccgggcca gtaaggagta ccccagttca
    39481 tcaggaagct ggcttgccac tttcggcaac gcgaccggtt taagcttcaa tgactttgtc
    39541 tggtgatttg taagtgcgct taaccgtgcc tcaataattt tcattttccc cgcgacatcg
    39601 ttgagctgct gccgggtttt gctggcatta atatcggttt ccacaccttc aactgaagaa
    39661 gtaatcccgt tctgatatag ctggcgatcg gtcgcgataa tggtgttctg ctctttttcg
    39721 atttgctgca agaccgtgtt taacgccgcc tgggtttgcc actcccagta caggcgggct
    39781 acgctgccag ccagcaattg gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta
    39841 cccaggcggg cagtaacctc cgcccgattc tttccccaga tatcgagatg ccagcccgcc
    39901 gttaagccaa aagttccgtt ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc
    39961 agagcaaacg gccccattag cccttctgcc gacatttttt gccgctccat atccgcagaa
    40021 aagtcgatct gcggaccatc ctgagtggca attgccttcg cctgggcttc agctagctga
    40081 atgcgctgtt cagccacctg catatccggt gcgttctgta gtgcattgtt aattaatgaa
    40141 gtgagttgat tatcgtgata ctccagccac cattggctgt ctggccaacc atttttcagc
    40201 gccgtgggta atgcggtatc aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg
    40261 gtttcatgca taggcgcaca cccggtcagc atcagtaaca gcggaaaaca ggcgatggct
    40321 ggataaaagg aatcacgatt catgggggaa taatcaggta agaaaaggtg cgcggagatt
    40381 accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat acatcagtag caataaaacg
    40441 acatatccag aaaaatatac actaagtgaa tgatatcttc cgatttatct taatcgttta
    40501 tggataacgg caaagggctt cgttttttcc tatacttatt cagcactcac aaataaagga
    40561 acgccaatga aaattatact ctgggctgta ttgattattt tcctgattgg gctactggtg
    40621 gtgactggcg tatttaagat gatattttaa aaatcagata tgacatataa gttgtcaggt
    40681 caggaactag atattttgtg tacttgattt gcaaacagct ccggtgttgg tggcgtcaaa
    40741 atcgctgaag cgttccccga aggccggggc agcccacatg gatgtgggct gagggcgcgt
    40801 cttacaagga cgtttcctcg cgcccgaccc ggtagccgaa ggggataagc tg
//
LOCUS       DAZGCQ010000038        40534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000038 DAZGCQ010000000
VERSION     DAZGCQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.038"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            406..687
                     /locus_tag="ACHK07_002128"
     CDS             406..687
                     /locus_tag="ACHK07_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8102456.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            693..1181
                     /locus_tag="ACHK07_002129"
     CDS             693..1181
                     /locus_tag="ACHK07_002129"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8102457.1"
                     /translation="MFQLRPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHNIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(1421..3253)
                     /locus_tag="ACHK07_002130"
     CDS             complement(1421..3253)
                     /locus_tag="ACHK07_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8102458.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTTHADINTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DATALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTETLA
                     AARTHSDENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHS
                     DENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHSDENNKR
                     TLISANTYADQKETAINNRTDGLLMTERQARTAGDAATLAVARTHSDENNKRTLISAN
                     TYADQKETAINNRTDGLLTTERQARIAGDAATLATAAAYSDAGDSLTLKQATAYSDIS
                     TRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYVAENSFSYGIGVG
                     NYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            3485..4975
                     /locus_tag="ACHK07_002131"
     CDS             3485..4975
                     /locus_tag="ACHK07_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO8102459.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKYHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGLRGTGYDTSGRVQENGHA"
     gene            4968..6449
                     /locus_tag="ACHK07_002132"
     CDS             4968..6449
                     /locus_tag="ACHK07_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO8102460.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESGIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            6517..7554
                     /locus_tag="ACHK07_002133"
     CDS             6517..7554
                     /locus_tag="ACHK07_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8102461.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPIRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(7750..7825)
                     /locus_tag="ACHK07_002134"
     tRNA            complement(7750..7825)
                     /locus_tag="ACHK07_002134"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7790..7792),aa:Phe,seq:gaa)
     gene            complement(7931..8638)
                     /gene="yqgA"
                     /locus_tag="ACHK07_002135"
     CDS             complement(7931..8638)
                     /gene="yqgA"
                     /locus_tag="ACHK07_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO8102462.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHEPF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            8791..8874
                     /locus_tag="ACHK07_002136"
     CDS             8791..8874
                     /locus_tag="ACHK07_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102463.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            9037..11172
                     /gene="speC"
                     /locus_tag="ACHK07_002137"
     CDS             9037..11172
                     /gene="speC"
                     /locus_tag="ACHK07_002137"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO8102464.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAVEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(11222..12478)
                     /gene="nupG"
                     /locus_tag="ACHK07_002138"
     CDS             complement(11222..12478)
                     /gene="nupG"
                     /locus_tag="ACHK07_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO8102465.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(12680..13759)
                     /gene="mltC"
                     /locus_tag="ACHK07_002139"
     CDS             complement(12680..13759)
                     /gene="mltC"
                     /locus_tag="ACHK07_002139"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO8102466.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(13824..14099)
                     /gene="yggX"
                     /locus_tag="ACHK07_002140"
     CDS             complement(13824..14099)
                     /gene="yggX"
                     /locus_tag="ACHK07_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO8102467.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(14127..15179)
                     /gene="mutY"
                     /locus_tag="ACHK07_002141"
     CDS             complement(14127..15179)
                     /gene="mutY"
                     /locus_tag="ACHK07_002141"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO8102468.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            15340..16059
                     /gene="trmB"
                     /locus_tag="ACHK07_002142"
     CDS             15340..16059
                     /gene="trmB"
                     /locus_tag="ACHK07_002142"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO8102469.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            16059..16385
                     /gene="yggL"
                     /locus_tag="ACHK07_002143"
     CDS             16059..16385
                     /gene="yggL"
                     /locus_tag="ACHK07_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO8102470.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            16569..17288
                     /gene="yggN"
                     /locus_tag="ACHK07_002144"
     CDS             16569..17288
                     /gene="yggN"
                     /locus_tag="ACHK07_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO8102471.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            17464..18510
                     /gene="ansB"
                     /locus_tag="ACHK07_002145"
     CDS             17464..18510
                     /gene="ansB"
                     /locus_tag="ACHK07_002145"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO8102472.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            18627..19634
                     /gene="yggM"
                     /locus_tag="ACHK07_002146"
     CDS             18627..19634
                     /gene="yggM"
                     /locus_tag="ACHK07_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO8102473.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINKACDRWKKDTEQLRRNGVI
                     K"
     gene            complement(19790..20926)
                     /gene="hemW"
                     /locus_tag="ACHK07_002147"
     CDS             complement(19790..20926)
                     /gene="hemW"
                     /locus_tag="ACHK07_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO8102474.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(20919..21512)
                     /gene="rdgB"
                     /locus_tag="ACHK07_002148"
     CDS             complement(20919..21512)
                     /gene="rdgB"
                     /locus_tag="ACHK07_002148"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO8102475.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(21520..21810)
                     /gene="yggU"
                     /locus_tag="ACHK07_002149"
     CDS             complement(21520..21810)
                     /gene="yggU"
                     /locus_tag="ACHK07_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO8102476.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(21807..22373)
                     /gene="yggT"
                     /locus_tag="ACHK07_002150"
     CDS             complement(21807..22373)
                     /gene="yggT"
                     /locus_tag="ACHK07_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO8102477.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(22391..23095)
                     /gene="yggS"
                     /locus_tag="ACHK07_002151"
     CDS             complement(22391..23095)
                     /gene="yggS"
                     /locus_tag="ACHK07_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO8102478.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            23113..24093
                     /gene="yggR"
                     /locus_tag="ACHK07_002152"
     CDS             23113..24093
                     /gene="yggR"
                     /locus_tag="ACHK07_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO8102479.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRQGISLALRLLPSHYPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(24277..24693)
                     /gene="ruvX"
                     /locus_tag="ACHK07_002153"
     CDS             complement(24277..24693)
                     /gene="ruvX"
                     /locus_tag="ACHK07_002153"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO8102480.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(24693..25256)
                     /gene="yqgE"
                     /locus_tag="ACHK07_002154"
     CDS             complement(24693..25256)
                     /gene="yqgE"
                     /locus_tag="ACHK07_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO8102481.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(25365..26315)
                     /gene="gshB"
                     /locus_tag="ACHK07_002155"
     CDS             complement(25365..26315)
                     /gene="gshB"
                     /locus_tag="ACHK07_002155"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO8102482.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(26328..27059)
                     /gene="rsmE"
                     /locus_tag="ACHK07_002156"
     CDS             complement(26328..27059)
                     /gene="rsmE"
                     /locus_tag="ACHK07_002156"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO8102483.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(27139..27846)
                     /gene="endA"
                     /locus_tag="ACHK07_002157"
     CDS             complement(27139..27846)
                     /gene="endA"
                     /locus_tag="ACHK07_002157"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO8102484.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(27941..28438)
                     /gene="yggI"
                     /locus_tag="ACHK07_002158"
     CDS             complement(27941..28438)
                     /gene="yggI"
                     /locus_tag="ACHK07_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO8102485.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(28515..29909)
                     /gene="galP"
                     /locus_tag="ACHK07_002159"
     CDS             complement(28515..29909)
                     /gene="galP"
                     /locus_tag="ACHK07_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO8102486.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(30346..31500)
                     /gene="metK"
                     /locus_tag="ACHK07_002160"
     CDS             complement(30346..31500)
                     /gene="metK"
                     /locus_tag="ACHK07_002160"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO8102487.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            31556..31807
                     /gene="yqgD"
                     /locus_tag="ACHK07_002161"
     CDS             31556..31807
                     /gene="yqgD"
                     /locus_tag="ACHK07_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO8102488.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            31918..32061
                     /gene="yqgG"
                     /locus_tag="ACHK07_002162"
     CDS             31918..32061
                     /gene="yqgG"
                     /locus_tag="ACHK07_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO8102489.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            32155..32286
                     /gene="yqgB"
                     /locus_tag="ACHK07_002163"
     CDS             32155..32286
                     /gene="yqgB"
                     /locus_tag="ACHK07_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO8102490.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            32295..34271
                     /gene="speA"
                     /locus_tag="ACHK07_002164"
     CDS             32295..34271
                     /gene="speA"
                     /locus_tag="ACHK07_002164"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO8102491.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            34417..35148
                     /locus_tag="ACHK07_002165"
     CDS             34417..35148
                     /locus_tag="ACHK07_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102492.1"
                     /translation="MKKWKVRSALIALVVLLVGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            35284..36204
                     /gene="speB"
                     /locus_tag="ACHK07_002166"
     CDS             35284..36204
                     /gene="speB"
                     /locus_tag="ACHK07_002166"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO8102493.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(36410..37168)
                     /gene="loiP"
                     /locus_tag="ACHK07_002167"
                     /gene_synonym="yggG"
     CDS             complement(36410..37168)
                     /gene="loiP"
                     /locus_tag="ACHK07_002167"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO8102494.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            37446..39437
                     /gene="tkt"
                     /locus_tag="ACHK07_002168"
     CDS             37446..39437
                     /gene="tkt"
                     /locus_tag="ACHK07_002168"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8102495.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            complement(39483..40391)
                     /locus_tag="ACHK07_002169"
     CDS             complement(39483..40391)
                     /locus_tag="ACHK07_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO8102496.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVLLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
BASE COUNT         9741 a        10020 c        10818 g         9955 t
ORIGIN      
        1 gagttgcctt tagtgtaaat ttacgttaaa aatcagaaga gcacgtttcc gggccgttca
       61 tgccaccacg tgaacattcg tcgcggaata ccggagacgt gctcattata ataaccaata
      121 tacataattc ctctttgaca ccccctgaat tcgggggatt tttttgcccg ggtatgttca
      181 ctcacagata aaatcccggt gtactccgga tgtcgctgct ggtaaataat ctgagctttt
      241 atctgtatgc aatggtcaaa gatcgtggcg ttaacatccc ccggatagtt ttgtctgaat
      301 ctgatttttc ttttcaggca gaatgcctgt ctgccgtccc ggtgtcagag tgtaatttgc
      361 acaatacggc aatttgccgt acagtgtatt caggaggtac aactcatgcc atctgcaaca
      421 caaactgccc gactggaagc aaggatcagc acagaactac atgccatgct gaagcgcgct
      481 gcagaaatcc aggggcgcac aatgaccgat tttatcattt ctgccgttca ggatgcggcc
      541 caacgtgcta tcgaacaaac cgatataatc cgactgtccc ggacagatca ggaaattttt
      601 gctcaggcat tactgtcacc aacagagccg aatgatgcgc ttaaacgggc ctttaaacgc
      661 cgtaataata tgctgtacac aaaataaata taatgtttca actgagacca tttaacagca
      721 catatgacag aacctcattt ttatgtggct ctgatgctct ggaccgttac ataaaagagc
      781 atgtcacaca ggatattcga cgacgcatcg cggcctgttt tgtcgctgta gataacgatc
      841 ataatatcat cggatactat acgttatcag cggccagtat attattaact gacttacctg
      901 agcatttgag aaaaaagctt ccgcgatatc ccactattcc ggctattcgt atgggacgac
      961 tggcagtaag tctgtcatca aaaggaacgg ggcttggggg agcactttta gctgatgcgc
     1021 tatatcgtgc atacacatct gaaattgcag catatgcgct tattgttgat gcaaaagatg
     1081 acacggcagt cgcattttac aaacattatg gctttatggc gttcccggag tccggaagaa
     1141 ctttattcat gccattggca tcattatcca caataaaata aacagaataa atcagggaaa
     1201 tacggtgctt gtggcaacgt cagcccacga agttaaatgt taacgggcaa cacggcggca
     1261 ggaaatctgc cgccggattg ttgtgaaggc tgaccacact gcgcgccctt tttccttgat
     1321 tatcctgctc agtccagtgg tctgttccgg atggggctgt gttgcgtact gaattaatgt
     1381 gattatggaa taccataatg ccgtatgtac gcaagattat ttaccagccg ccggcaaccc
     1441 ctgcaccaat cgcggtgtta tgagatgagt cccaggagat gttgaggcgt atattggtat
     1501 tacgtgatgt tttatactga atgcctgcgg ccacagcatt agcgttctga tagttaccca
     1561 cgccgatccc gtatgaaaaa gaattttcag ccacataggg gatggaggat attgctgtta
     1621 ctccggcgat gcctgcatta agtctttttt ctgcccgttc aattttattg ctcaggtttc
     1681 tgaatttgtt gtcggtataa cgattggcct ctgacagtgc ccggcgggta ctgatatcgc
     1741 tgtaggctgt tgcctgtttc agtgtcagtg aatccccggc gtcactgtat gccgctgctg
     1801 ttgccagagt tgcggcatca cctgcgatgc gtgcctggcg ttcggtcgtc agtaatccgt
     1861 cagtacggtt attgatggcg gtttctttct ggtcagcata ggtattcgca ctgattagtg
     1921 ttcttttgtt gttttcatcg ctgtgcgttc tggcaactgc cagcgtcgca gcatcacccg
     1981 cggtgcgtgc ctggcgttcg gtcatcagta atccgtcagt acggttattg atggcggttt
     2041 ctttctggtc agcataggta ttcgcactga tcagtgttct tttgttgttt tcatcgctgt
     2101 gcgttctggc agcagccaga gttgcagtat cgcctgcggt gcgtgcctgg cgttcggtcg
     2161 tcagtaatcc gtcagtacgg ttattgatgt cggtttcttt ctggtcagca taagtattcg
     2221 cactgaccag tgttcttttg tcgttttcat cgctgtgcgt tctggcagca gccagagttg
     2281 cagtatcgcc tgcggtgcgt gcctggcgtt cggtcgtcag taatccgtca gtacggttat
     2341 tgatgtcggt ttctttctgg tcagcatagg tattcgcact gaccagtgtt cttttgtcgt
     2401 tttcatcgct gtgcgttctg gcagcagcca gagtttcagt atcgcccgcg gtgcgtgcct
     2461 ggcgttcggt cgtcagtaat ccgtcagtac ggttattgat ggcggtttct ttctggtcag
     2521 cataggtatt cgcactgacc agtgttcttt tgtcgttttc atcgctgtgg gctctggctg
     2581 cagccagcgc tgttgcatca ccttctgttc gggctctggt ttcagttatg acctgaccag
     2641 agagggtatt aagctgattc tgcaggttgt ttgttgattt tattctttct ccagcttccc
     2701 ggttaacagc actggtcatc tgcatcagat ttaccgcatc atggtcatct ttcccctgtg
     2761 ccagatttgt gatgcggcgt ttcagtgtgt cagagccgac ggatacgata ttgtttccgt
     2821 tagcactgga atgactgccc agtgccacgg agtcgttccc ggttacggac gatgtcagac
     2881 ctatggcaac agatgaatca ccgttaattc tggtggcatt accgaggctg acgctgtaac
     2941 catccagcac aatatcttcg cccacttttc tgatcaaacc ggaagctttg accgtggagg
     3001 aattaccaat aacaatgttg ttataccctc cctccacgga attactgtta cccacaacca
     3061 gactgtttga ctctgatgtg acagtatttt cactgccgta aatatttccg gagaatcgtg
     3121 ttgttgtatt gtttttttct ccggtgatgt tcggtgtgtt gatatcagca tgtgttgtct
     3181 gacagacgat ggcggctgac agcatagctg caactgttaa tgaataagaa acgtgttttt
     3241 ttacttcgga cattattttt tcctgccaga ttatgacctt tcaggtcccg gtatcctgga
     3301 taagcgcggc agacaggaga ccacctgcct ttctgaggac aggatgagaa gcatccggtg
     3361 tgatggcgat gattaattca tcatatgacc agggaaattt ttctgttgct gtacagagtt
     3421 atttgtggtg ggcatacccc tgatgcatca gattacggaa atgtttttcc ggagaagaga
     3481 atgcatgtct tttaatccca cagatgaaca gtacgccatt attaactggc aggggcagca
     3541 actggttgtg aatgcgtttt ccggcacagg gaagacgagt acgctggtgc agtttgcccg
     3601 ggccagtccg gacagccgta tgctctatct tgcctataac cgggcaatac gtgatgaggc
     3661 agagcgtaaa tttcccttta acgtggaatg cagaacgtca catcagctgg cgtatgcccg
     3721 ggttggaaga cactaccgtc atcgtctggt tccgggatta cgtattactg atgtggcgag
     3781 aaagctgaat acacgatact gggctctggc acgggtggca gggacggggc tgaatcactt
     3841 tatctgttct gccgatgcgg taccgggcct gcatcacctg ccggataagg atgagatgcg
     3901 gggtgttccg ccagcagatg cgcttcgggc ggtgcagttg ctctggaacg aaatgagcaa
     3961 ccccgggggg acgttcccgg tgacgcacga tacttatctg aaactcttcc agttgtccgg
     4021 tgcagacctg tctcaccgat gggacaccat cctgtttgat gaggcgcagg atgcgaatcc
     4081 ggtgaccact gctctggttc tggggcagaa gtgtcgggtg gtccttgttg gagattgtta
     4141 tcaacagatt taccgctttc ggggtgcgaa taatgcgctt tcccatcctg cactgaagaa
     4201 tgcggatcgg ttgtggctga cacagagttt ccgttttggt ccggcggtgg ctcgtatggc
     4261 aaatctgctt ctgcagcgtg aaggtgaaac acgagaggtg aaaggttgtg gtggtgatga
     4321 tgaggtattg ctgaaatacc atgcgcgtga acatctgcag gggcattaca cggtactcag
     4381 tcgtactgtc gcgggtgtga ttggtactgc gctgatggcg gcaatgaaag ggcaaaaggt
     4441 ttactgggtg gggggaatcg aaggttacag gaccggggaa cttgaggatc tctactggtt
     4501 ccaggttgat atgccggaac ggatgcattc agacaggctg cgccgggact acaggaattt
     4561 tgaagaatat aaatatattg caaaatccac gaaggatgtg gagatgaatc agtcccttcg
     4621 gcttctggag ttgtgttttc cgttaccgaa aaaactggaa cttctccggc agtatacggt
     4681 aacgaatgag caggatgcag atatcacggt gtcaacggca caccggagca aggggctgga
     4741 gtggccggtt gtaatgctgg atgaggattt ccaggatatc acggatccgc tgatgacgga
     4801 ggccgagcgt catgatgaaa cctgtctgtt atatgtggct gtcacccggg ccctcaggac
     4861 gctggtactg aatgacctga tgcaccaact ggcagaagaa cagcagaaaa ctgaaggtct
     4921 gcgggggacc gggtacgaca catcaggtcg ggttcaggag aatgggcatg cttgaacgcc
     4981 tgaaaaggtt atttcctgtg aagacagaat gttctgatgt ccggacggat attcccgggg
     5041 aaacggtgcc ggaggggtat tttacaccat tgccggctga tgtactggta tccacaccgt
     5101 tcaggaaaag gtgtctgaaa cagttatggg aaaacagttc catgccggtg gatatgtatc
     5161 agcgcctttg tcttgatcct gtgcatcagt tgttgtctgt cagtcagaat ctgccggcca
     5221 cagcagaagg tcgctggggt tacaccggtg gttttggtga cctgaccctg cagttcacga
     5281 cgtatgctgt ccggctggcc agaggcagaa tgtttccacc gggagcgaca gcagaagagc
     5341 aggccgcgca ggggagtgtg tggaacatgg tggtgttctg gtcggctctg ttttatcacc
     5401 tgccgttgct ggcggaactg gaaggcgaac aggccgacgg cagatgctgg cagccgggga
     5461 tatctgtacc tgagggcgta taccggttcc ggttcaggaa gatgccctgc cagggaacgg
     5521 agggacggca actggcagcc ctgatggcgg gacaactgct gcccggagag gcgggagcct
     5581 ggcttgccac tgtcccgggg gcattacaga atcttgccgg agcagtctgg catcagcatc
     5641 ctgaaatggc actgatacgt acagtattac aggaagctat ggaggaagtc gtgagtcccc
     5701 tcgctgcagg agatgtggcc gaagttccgg tggctgaggt tgttccggta ctgctgcagt
     5761 cagcagcaat ttcagcagaa agcgtcattc agccgttgcc ggttgtacag tcgccagtgg
     5821 aggatgccga aaatgagggc cggacagcgg aacaggaggt ggatgagggg aacgaaactg
     5881 atgctgatac cggaatgctc cttacgctgt tttctgctgc tgatttgtcg gatgagggag
     5941 ggcagatagc gtcaggcgtg ccgccggata aagaaagcgg tattgagtta ccggaagaga
     6001 gcggatgtgt aacagaaaat cagggaaacc gtatcccgga aacagtggaa aaaacggcag
     6061 agttttccct gacagaggat agcagggtag agagtgaatt tatcagatgg ttaaaagagg
     6121 ggctgaggga gggttcactt tcggttaaca gcgttgattc aaatgttcat gttgtttccg
     6181 gatatgtttt tctgtgtgcc ccggggattt tttatcgttt tctgaaggag agtggttccg
     6241 ggcaggatcg tcgttatgtt cagtcgctct ttgaacgggc aggtatccat aaggtacgtt
     6301 caggagagcg tttttttcag gcccgtcttt atagtgacgc agaacattcc ggacgttaca
     6361 aaatggtttc gggatatctg gttaagggca ggtttctgta cgggaatatt acggttcccg
     6421 gagacagccc gcttattgtt ttcgggtgag taaaggcgat gatgtgcaga atttgaccgg
     6481 agagtgcggg agcacagtaa acaaggggaa tatataatgc agaatgacga ttccgtcaca
     6541 aaaatcacct gggaacaact gctggaagag tacttttttt caaagaatct tcggccggat
     6601 acagagtgga gctactgcaa agttgtcagg gtattcaggg aatttatcgg tgatattttg
     6661 ccgggggagg tgactgtccg tgatgttcag cgataccggc gttatgtcct gaaagagcag
     6721 aagcgcaggg aggtgacctg gaataacaag gtggcccact tgcgggcaat tatgggattc
     6781 gggatcaaga tgaaactgct gcctcagaca gaaaacccgt tggtggaagc atcagttgcg
     6841 agtactgaaa aaaagaaaaa gacactgagt gacaatcaga tgctcagggt ttacctggtt
     6901 gtgcagaggt ttgcagaagc ggaagagcag aacatggtga atagtcgtcg acgcaatgct
     6961 atataccctg cctggtactg gctgacagtt ctggatgttc tgcgttatac cggaatgcgc
     7021 tttaatcaac tgaagcatat ccgtgtgagg gatgtctgtc cggatgaaag cataattgat
     7081 cttaagctgg agggaagtaa aactcacagg gaatggggcg ttcctctggt ggctcccctg
     7141 aaggtatcga tggaaaaact gctggagcgg gcccgtcagt gcggggcagg tgatgatgac
     7201 tatctttttg acgtctgtcg tttcagtgag ccgatctctg ccgacagata tcagtactgt
     7261 cccattcggg cgcatcagac cgtacgttca tttttccggc gactttcgcg agaatgtggt
     7321 tttcctgttt caccacatcg tttcagacat acgctggcaa ccagactgat gaaatatcct
     7381 gaacgtaatt tacatctggt caaatccata ctgggacaca ggagtcttac aacgaccatg
     7441 gggtatgttg atgtctgtat ggagtctgca gcgcagacac tggaagatgc cctgagtttg
     7501 tatacagatg agcgcacagt gagcaagatt tgcaaagggg ggaataaatc ttgacaggct
     7561 gaatcagcga tgtaagcata cagtgttaac gagaacaggg cgtctccgga aaagacaccc
     7621 tgtttgagga tacgatacca aactcaatca gtgtaaccac aacttacaca aacggagacc
     7681 ctttgatgcc gcatcctgta ttaaccgccc gtcaaggatc tcatccctta accagcgtgt
     7741 tctggtatat ggtgcccgga ggcggaatcg aaccaccgac acggggattt tcaatcccct
     7801 gctctaccga ctgagctatc cgggcaacgg ggcgcattaa acctgattcg cctcgtctcg
     7861 tcaatcaaat ttcttcaatt tactgcagac tgcacaatct atcatcactt tgccgctatt
     7921 gcacgcattg tcaggcaaac caggcggtcc aggctgcgga aagcggcatt gccagaagga
     7981 gtgctggaag catgttgacc accgggaaca ttttaatgcc acagatgcgt aatccggttg
     8041 ccagcagcaa taaaccgcct acggcgctga agtctgccat catcgacggt gtggtcagcg
     8101 gtaatatcag cgcggcagcc cacgccagcg ttaactggat gatcagtaat gggatactaa
     8161 ttaccgacac cgcaataccc agcgagcagg cgaagatcat cgccgtaaag aaatcaagaa
     8221 atgacttggc gattaaaata ctcggatcgc cggtcatccc ttcgttcatc gccccgaaga
     8281 tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac ataattctga ataaaaggtt
     8341 catgcgctgg cttcttacgt gagtgacgaa acagattttg tgctttggcg accgctgtat
     8401 tgacgccttt ctccagcaga caaatttcgc cgattaatgc tccgagtaag gttgctaaaa
     8461 ccatcgctgg aaggttggcg catttcacca ccagtaaaat accaatcccc agcgatgcca
     8521 gaccaaaaat tgatgtcatg gagacgcgga tacgttccgg taagcgttgg ctaagcagtg
     8581 caccgagcat gccacccagt aatattgcac ttgcgttgat aaaagggccg atgaccacaa
     8641 gagttcctgt taactgtatt gtttgatttt cttattatga actttttgcc ttgttgatgg
     8701 cttgtctggt cgcaaagtcc tggcttgcac gtcttagcga aaggtgccat gattgcgcga
     8761 attttctcct ctctgtacgg agtttgcccg atgcacgcca tctccttaca ttctctcgct
     8821 tatcgccgtt tcgcgcgaaa cccttctctt tttatgctac tgctcatgcg gtgaagttaa
     8881 cgcacgttca ctgcaggaca acagtaaaat cagagcgttt ctgcttttac tgatgtctgg
     8941 cggtcggagc tggtgaccag tttgacccat atctcatggg gcagggtttt acaccttgtc
     9001 cggtattctt acttccccga aacgggtttt gcgcttatga aatcaatgaa tattgccgcc
     9061 agtagtgaac tggtatcccg actttctact catcgccgcg tggtggcgtt gggagatact
     9121 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     9181 acgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     9241 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcattg gctggagctg
     9301 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     9361 cagtacgttg agatgggcaa cagcaccttt gcttgccctg ggcatcaaca tggtgcgttt
     9421 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     9481 gccgatatgt gtaatgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     9541 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtactg
     9601 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tattaacgcg tggcgatctg
     9661 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     9721 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     9781 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     9841 gccgacctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggcact
     9901 gtctataacg cccgtcaggt gatcgatacc attgggcatc tgtgtgatta cattctgttt
     9961 gattccgcgt gggtcggtta cgaacagttt atcccgatga tggcggatag ctcgccgctg
    10021 ctgttagaac ttaacgaaaa cgatccaggg atctttgtga ctcagtcggt gcacaaacag
    10081 caggcgggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
    10141 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
    10201 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
    10261 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
    10321 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
    10381 gattatccga catcagtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
    10441 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
    10501 ttactcacta caccaggtat cgatgccgaa accggcgagt atagcgactt tggcgttccg
    10561 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
    10621 aactccattc tgttcttatt aactccggcg gaaagccaag agaagctggc acaactggtg
    10681 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
    10741 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
    10801 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
    10861 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
    10921 cgcggtgacg tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggtagaaggg
    10981 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
    11041 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
    11101 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
    11161 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
    11221 attagtggct aaccgtctgt gtacctgtcg ggacacgaac gtgtttatat ttgaacatcg
    11281 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
    11341 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
    11401 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
    11461 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
    11521 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
    11581 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
    11641 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
    11701 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
    11761 tgtcgaagct gtgcaggaag gtattaccga acatgttggt aatctgcagt tccgcgccca
    11821 gcagcattga gaagatgaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
    11881 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
    11941 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
    12001 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
    12061 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
    12121 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
    12181 atatcacaag gaacatcgcc tccggcgtcg tgacctgtgc cgccatgaac agcgtgatag
    12241 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
    12301 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
    12361 tagaagcacc gtcaaacttc agggtaacaa acatatagga accgagggtc gtcagccaac
    12421 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
    12481 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
    12541 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
    12601 acattcctaa tttgcaaaac gaaattgtga tatggataac atttttgccc ctgagcatcg
    12661 tcaggggcgg ttagtggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
    12721 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtttga taaacatcgc
    12781 ccggcgtcat ggtgttaata atattagccg cctgaatctt atcattcgaa aagactcgca
    12841 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgg gatgtagggt
    12901 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
    12961 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
    13021 ggaacacatc tttcccggca gtatgttgta ccacctgcat taaccccagc gcatcggaac
    13081 ggctgaccgc atacgggtta aaggaagatt cagtctgcat aattgccaga atcagcgact
    13141 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
    13201 tatcaaggtg gttcggcacc atgttaatgg tgacgctgta gatgatacgc agcccgttgc
    13261 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgcttgcg cgaccttccc
    13321 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
    13381 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcaata
    13441 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atggtttcga
    13501 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
    13561 tcacgtagtc cttaggacca gcgatcacca cctctttgaa gccccagatg ttctcaatat
    13621 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
    13681 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
    13741 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagctgcc
    13801 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
    13861 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
    13921 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
    13981 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagttcgcc ggggtacagc
    14041 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
    14101 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
    14161 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
    14221 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
    14281 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
    14341 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
    14401 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
    14461 tgctgtaaca ggagaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
    14521 ggataaagcg accagctatt gttggtggcg gcaatacatc cgttttgtag cggacagagc
    14581 gaacatttcg gcttcgaacg cgtgcaaatc atcgcgccca aatccatcat cgcctgatta
    14641 aaccgttcca cgccgaccgc gggcgtcacc tgctcgctta aactccataa tttattctcg
    14701 acctcttttt tcccaggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
    14761 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc gcctgcggtg
    14821 gaacgcccga cgcccggcag tgctgcaact tcctcaaagg tttccgggaa tttaccgccg
    14881 tgtaaggtcg ccacttgttg tgccgcttta tgcagattgc gcgcgcgggc gtaatagcca
    14941 agccccgtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
    15001 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
    15061 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagagtt
    15121 tttcgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
    15181 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
    15241 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
    15301 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
    15361 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
    15421 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
    15481 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
    15541 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccccgag
    15601 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
    15661 catgaagagg gcttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
    15721 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
    15781 aaagcgcgcc ataataaacg ccgtatcgtt caggtgccgt ttgccgaact ggtaaaaagc
    15841 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
    15901 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
    15961 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
    16021 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
    16081 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
    16141 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
    16201 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
    16261 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
    16321 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
    16381 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcat
    16441 gcctacaaag atgcacgatc gagtaggcgg gataaggcgt ttacgccgca tccggcatgg
    16501 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt tgttcttgag
    16561 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
    16621 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
    16681 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
    16741 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
    16801 ctgaactacg tagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgagaaag
    16861 cccgtattgc tctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
    16921 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
    16981 gcagcgatgg cctgacgttc cactataaag ccattgatca ggttcgcgct gaaggccagc
    17041 aattagtgaa tcaggcaatg ggcggaattt tacaggacag cattaatgaa atgggcgcga
    17101 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
    17161 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
    17221 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
    17281 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
    17341 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
    17401 cgtcaaattt cccatacaaa gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
    17461 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
    17521 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
    17581 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
    17641 gcggtgccgc aactgaagga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    17701 tcccaggaca tgaacgatga tgtctggctg acactggcga aaaaaattaa caccgactgc
    17761 gataaaaccg acggcttcgt cattactcac ggtaccgaca cgatggaaga aaccgcttac
    17821 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    17881 tccacctcca tgagcgcaga cggtccattc aacctgtata acgcggtagt gactgcagct
    17941 gataaagcct ccgctaatcg tggcgtgctg gtagtgatga acgacaccgt gctggatggt
    18001 cgtgacgtca ccaaaaccaa caccaccgac gtagcaacct ttaagtctgt taactacggt
    18061 ccgctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    18121 accagcgaca cgccgttcga tgtctctaag ctgaatgaac tgccgaaagt cggcattgtt
    18181 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    18241 ggcatcgtca gcgctggtgt gggtaacggc aacctgtata aaaccgtatt tgacaccctt
    18301 gcaaccgccg cgaaaaacgg cactgcagtc gtgcgttctt cccgcgtacc gacgggcgct
    18361 accactcagg atgccgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    18421 aacccgcaaa aagcgcgcgt tctgctgcaa ctggctctga cgcaaaccaa agatccgcag
    18481 cagatccagc agatcttcaa tcagtactaa tcgcctcgcc ccggtatcgt gccggggctt
    18541 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    18601 aaattattcc gcgcgagggt tttcgggtga aaaagcaatg gattgttggc acggcgctgc
    18661 ttatgttgat gactggtaat gtctgggcag atggcgaacc gccaactgaa aatatcttaa
    18721 aagatcaatt caaaaagcag tatcacggca ttctcaaact tgatgccatc accttaaaaa
    18781 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    18841 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctggaa cagacctgga
    18901 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    18961 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    19021 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    19081 ttagtcagct tgagtctgcg ttgaataacc agaagaactc gattcctgcg ctggaaaagg
    19141 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    19201 gaaaagatgc aaacggtaag caaatgaccc gcgaagacgc ctttaaaaaa attcaccaac
    19261 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    19321 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    19381 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact tttctgcaat
    19441 cacaaaaact tagccgtaaa ttgcaggatg actggataac gctcgaaaaa gggcaatatc
    19501 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaagattg
    19561 acgatatcaa taaggcctgc gatcgctgga aaaaagatac cgaacaactg cggcgcaatg
    19621 gggtgattaa gtaagttttg atatagcgca cgatttgtag caggatgcgg cgttgacccc
    19681 gcatccggca atcaacgtct gatgcgacgc tagcgcgtct tatcagacct acaaccgttg
    19741 ccacatgtag gctggataag gcgttcacgc cgcatccggc aatacaagtt tactcagcca
    19801 gaaaaagctc cagcagcgaa tttaaaaaca acttcccatg ttccgttatc tgccagtaat
    19861 ccgcacattc tgtgagataa ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat
    19921 cttcgcaaag cccggtatac gcactaaact ctgcgcgtgg cgcggcctcc agcaagcgaa
    19981 agcggttcat aaagaactca aacggcttat ctgcagcttc gacatcacgc tggctttcca
    20041 gataccttcc ttgcataaaa ccacgcggat gacgcgtttt ggtggtacgc agaatgcgcc
    20101 catccgggaa ggtcactttg ccgtgcgcgc cgcagccaat accgatgtag tccccaaagc
    20161 gccagtagtt gagattgtgc tggcactggt aaccgggttt ggcgtaagcg gaagtttcat
    20221 attgctgata acccgctgcg gttaataact gatgcccctg ttcgaatata tcccacaacg
    20281 cgtcatcgtc cggcagtaca ggagggcgcg agccaaacag cgtattgggt tcgatggtca
    20341 gttgatacca ggaaagatgc ggcggattca gttcaatggc ctggcgcaga tcgccaagcg
    20401 cctcttccag tgattgatcc ggcagcccat gcatcaaatc aaggttaaaa ctacgcagcc
    20461 ccagcccgct tgccagattc gcagctcgtt tcgcttcttg cgggccatga atgcgcccaa
    20521 gtcgtttcag cttttcttcg ctaaaactct gtacgccaat agagatgcgg ttcacgccag
    20581 cacgctgata atcgacaaag cgatcggctt ctaccgtacc agggttcgct tccatagtaa
    20641 tttctgcatc cgctgccagc ggcaaacgcg cacgcacgcc gtccagcagc gtttgcatcg
    20701 ccgggccgga aagcaggctc ggcgtaccac cgccaataaa aattgtctgc acttcacggc
    20761 cctgagcgta agccacatcg ttgtccagat cgttaagcag atgctgaaca taatcgtcgt
    20821 gcggcacttc tcctttcaac gcgtgagagt tgaaatcgca gtacgggcat ttctgcacgc
    20881 accacgggat gtgaatgtag agactcagcg gcggtaattt aaccattacg taaagcgtcc
    20941 agcagcagtt tcaacgcctg accacggtgg gaaatggcgc tcttttgttc gcgagtcagt
    21001 tcggcagcgg ttttcccttc ggaaggtacg aagaagattg gatcataacc aaagccacca
    21061 gtgcccgccg gttcacgagt aatcactcct ggccagctac cgtggcacac cagcggagtc
    21121 ggatcttccg cgtgacgcag atacaccagc acgcagtgga aacgcgcctg acgttggtcg
    21181 tccggtacgt ctttcagtgt ttccagcagt ttttgcagat tcttttgatc ggtcgcgtct
    21241 tcaccggaat aacgcgcgga gtaaatgcct ggcgcgccac caagtacatc taccgccaga
    21301 ccggagtcgt cggcaattgc aggtaaaccg gtcacttttg ccgcatggcg cgctttcaga
    21361 atcgcgtttt cgataaaggt caggccggtt tcttcagcgg aatctacgcc gaggtctgtt
    21421 tgggccacga tatcaagacc gaagtcgcta agcagcgacg ccagctcacg cactttaccg
    21481 gcattgccgg ttgcgaggac aactttttgc ataggatacc taattaatta acgccgcgat
    21541 ttctggcggg atttgttgcg gattaatgat tttaatttgt ttgtggcggc caagttcgcc
    21601 tttttcaatc accacctggc ttttggcaac ccggaattgc ttaccgagaa acttcaccag
    21661 atgactgttg gcctggccgt caaccggcgg cgcggttatg gcgactttta cctcgtcgcc
    21721 atgtaaaccg acaatagaat cacggctggc tttcggctga atataaagcc gtaaaaccag
    21781 accgtcatcg ttaactgtta cggcactcat aacgccatcc acagccccgg cagcagcatg
    21841 tttccggttg cctgtaatac ttctgcgaca cccatattga tgacatacag cagcagaacg
    21901 aggatcatcg gcgagaaatc aatcccaccc attgccggta gcaggcggcg aatcgggcgc
    21961 agcagcggat cggccagctg aatcagcacg tattcaatcg ggctacggcc ctggcttacc
    22021 cagctcataa tcgccatcac cagcaggacc cagaaaatca gcaggccgat ggttttcagc
    22081 agaatcagta aaccggcaat ccagatgatt ggcaggaagg tcaccacttt aaacagcacg
    22141 atggctttga taaaactgag aatataggca accagtagcg aggcgctatc aatggggccc
    22201 attgccggaa taacgcggcg cagtggcccg ataattggct gcgttacctt cactacaaac
    22261 tgtgagaagg ggttgtaaaa atcacaatga gcccactgca tccagatgcg taataacagc
    22321 accatggtat acagctcaat gaccgttgaa agcaggaaag tcaacgtatt catggcgttc
    22381 ctttaattcc ttatttttta gagtaatcac gcgcaccaaa aattgcagtg ccgatacgaa
    22441 ccatcgtgct acctgccgca atggcggctt ccatatcgtc cgacattccc agagagagcg
    22501 tgtcgatatg cgggtagcgc gttttcagtc cggcaaatgc tacagccatt tggcgtgcaa
    22561 cttcaaactg ccttacatat tctgactcag gcgcagggat tgccatcagc ccgcgcagac
    22621 gtaaacgcgg tagttcagcg accgctgctg ccagctcgtc cagttctgcc agttgaatcc
    22681 cggacttact gttttcatca ctaatattaa tttgaatcag cacgttaaga gggggaagtt
    22741 ctgccgggcg ctgatcgttg aggcgggtag cgatgcgcaa acggtcgatg gtatgacacc
    22801 agtcgaaatg ctctgccacc aggcggcttt tattagactg caacgggcca ataaaatgcc
    22861 attctaatcc tgttacgccc agttcctgaa agtggcgaat tttatctacc ccttcctgaa
    22921 cgtagttttc accaaattga cgctgcccgg catcaatggc ttctgcgatg gcgctcgcag
    22981 gttttgtttt actgactgca agcagcgtaa tttcttctgg agaacggccg caacgcgttg
    23041 cagccgctga gattttgtcc cggacctgtg ccaggttatg cgcaatatcg ttcattttcc
    23101 gaggatctta gtatgaatat ggaagaaatt gtggccctta gtgtaaagca taacgtctcg
    23161 gatctacacc tgtgcagcgc ctggcccgca cgatggcgca ttcgcgggct aatggaagct
    23221 gcgccgtttg atgcgccgga cgtcgaagag ctactgcggg agtggctgga tgacgatcag
    23281 cgggcaatat tgctggagaa tggtcagctg gattttgccg tgtcgctgac ggaaaaccag
    23341 cgattgcgtg gcagtgcgtt cgcgcaacgg caaggtattt ctctggcgtt acggctgtta
    23401 ccttcgcact acccgcagct cgaacagctt ggcgcaccac cggtattgcc ggaattactc
    23461 aagagcgaga atggcctgat tctggtgacg ggggcgacgg ggagcggcaa atctaccacg
    23521 ctggcggcga tggttggcta tctcaatcaa catgccgatg cgcatattct gacgctggaa
    23581 gatcctgttg aatatctcta tgccagccag cgatgtttga tccagcagcg ggaaattggt
    23641 ttgcactgta tgacgttcgc atcgggattg cgggccgcat tgcgggaaga tcctgatgtg
    23701 attttgctcg gagagctgcg tgacagcgag acaatccgtc tggcgctgac ggcggcagaa
    23761 accgggcatc tggtgctggc aaccttacat acacgtggtg ccgcgcaggc agttgagcga
    23821 cttgtggatt catttccggc gcaggaaaaa gaccccgtgc gtaatcaact ggcaggtagt
    23881 ttacgggcag tgctgtcaca aaagctggaa gtggataaac aggaaggacg cgtggcgcta
    23941 tttgaattgc tgattaacac acccgcggtg gggaatttga ttcgcgaagg gaaaacccac
    24001 cagttaccgc atgttattca aaccgggcag caggtgggga tgataacgtt tcagcagagt
    24061 tatcagcagc gggtggggga agggcgtttg tgacgttgcc atgcccgatg tgcaagctta
    24121 tcgggcctac aaaggcaata aacgtctgat gcgacgcttg cgcgtcttat cagacctaca
    24181 accgctgcca catgtaggct ggataaggcg ttcatgtcgc atccggcaat aagacgcgat
    24241 gatattattt acactccgcc aggcttttaa atcgccttaa tatccctgct cgaaatagct
    24301 ttcgagaata ataaccgcag aggcagagtc gactttgcct ttgttaagcg cccgatagcc
    24361 gccctgttca aacagaccgg aacgggcttc cacggtgcta agacgctcgt catggagctt
    24421 tacttcaaca ccgaaacgac catgaatacg gttggcaaat ttacgcgctc tggcggttaa
    24481 cggctgttcg gtaccgtcca tattcagcgg caaaccaacg atgatttcgt ccggctgcca
    24541 ctctttcagt aaacgttcga tgagattcca gtccggcgta ccgtcctgtg ctttaattgc
    24601 aggcaatggg cgagcggtgc cggtaatgcg ttggccgacc gctacgccaa tgcttttggt
    24661 accgaagtcg aaggcgagta aggttccact catcaggcgt gtcctgccac accaggcatg
    24721 gtgagaatat ccacaccaat cagttttgcc gcctcgcgcc agcggtcggc aattggcgtt
    24781 ttgaacagaa tattcagatc tgccggggcg gttagccacg cgttatcgag aatttcttgt
    24841 tccagttgac ctttctccca ggaggcataa cccagagcca ccaatacgtc agacggttgt
    24901 ttatcggtgc cgagcgtttc cagcacatcg cgggaagtgg tcattaccgt gttgtctgaa
    24961 atgcgaatgc tggaagcaaa attagagggc ggagtatgca aaataaaccc gcgatcttca
    25021 gccagtgggc cgccgagcat aaccggttta tccagacgga ttgattcatc acgcggctcc
    25081 ggcgtgatct tcagcttttc cagaatccct tcaattttga gattttccag cggcttgttg
    25141 acgatgatcc ccattgcacc attggtatta tgttcgcaaa tgtagaccac ggaacgacgg
    25201 aaaatcggat cctggagagc aggcatggca ataagaaagt gatgctgtaa attcattgtc
    25261 agaggttctg tttcctggtt caaaaaagca acagcgccca gtatgcgtgg aaagaagggc
    25321 gctgtcactc agagtctcaa cgagatcctt ctcgctaagg tgggttactg ctgctgtaaa
    25381 cgtgcttcaa tggcatccat taacattcca gtgatcgaca ccggaaactc tgcttcaatc
    25441 tcacgaatac aggttgggct ggtgacgtta atttcagtca gacggtcgcc gatgatatcc
    25501 agaccaacaa aaatcagccc tttttctttc agcgtcggcc cgatctgacg ggcgattttc
    25561 cagtcacttt ccgtcagcgg acgaggttca ccgcgaccac cggcagccag attgccacgg
    25621 gtttcgcccc cctgcggaat acgcgccagg cagtacggta ccggctcgcc atccaccacc
    25681 agcacgcgtt tgtcgccatc tttaatggct ggcaggtaat tttgcgccat gcagtagcga
    25741 gtgccatgct cagtcagggt ttcggcaatc acgccgaggt ttggatcgcc ttctttcacg
    25801 cggaaaatcg acgcgccgcc cataccgtcc agcggcttaa gaatgatgtc gctgtgtttc
    25861 tcccagaacg cttttagctg cgctttattg cgcgtaacca gcgtttctgg cgttaagtca
    25921 gagaaccagg cggtaaacag tttctcgtta cagtcgcgca ggctctgcgg cttgttaacg
    25981 atcagcgtcc ctttctcttc ggcacgttcc agaatatagg tcgcgtagat aaactcggta
    26041 tcaaacggcg ggtctttacg catcaggatc acatcgagat cggccagcgg cagatcctgt
    26101 tcaccgacga atgaaaacca ctcttcgtag ttctgcttca cgttcagcgt gcgggtatgg
    26161 gcgcgggctt cgccattgat cagatacaga tcgcccatct ccatatagtg aagttcgtaa
    26221 ccacgacgct gtgcttccag caacatagcg aaactggaat ctttcttgat gttgatgttt
    26281 gcgatggggt ccatcacgat gccgagcttg atcattattc ttctccgtta gcccaaatcg
    26341 ccaaatcgta cttgtagcgc ggtaatggcg gtgagcgcag ttgtctctgt acgcaaaacg
    26401 cgaggtccca acaggatatc agtaaattga tagcgggcag tcatggcaat ttcatctgcc
    26461 gataaaccgc cttccgggcc aatcagcagg cggacgcgtt caaccggtaa aggcaacgta
    26521 ttgatgctgt tactggcgcg cgggtgaaga ttcagtttca gtccttcatc ctgctctgca
    26581 caccaggctt ccagatccat cgctggacgg atttccggca ctcggttacg accacactgc
    26641 tcacaggcag caattgcaat cttctgccac tgctggagct tcttgttcag acgttcacta
    26701 tccagtttaa cgccgcagcg ctcagaaaaa agtggcgtaa tgaggcttac accgagttcg
    26761 atcgatttct ggatcgtaaa ttccattttt tcaccacgcg acatcacctg accgaggtga
    26821 atatgcagcg gagattcgcg atcgtcgatc tggccttcca gcaccttcac ttccacgctt
    26881 tttttgctgg cgctggtaat ttcggcgtca aagacctggt tgctgccgtc aaacaattgc
    26941 aacgcctgcc ccggccccat gcgcagtacg cgcccgatat ggttggcggc atcttcgcaa
    27001 agcgcgatgt gagaatggct ggtcagtggt tcaggatgat aaatgcgggg gatacgcata
    27061 gttaaaaatc cgcgtcgtct ccccacgcgg ttgtacgcgt ggggtagggg ttaacaaaaa
    27121 gaatcccgct agtgtaggtt agctctttcg cgcctggcaa gcgcgttgca catacgggtt
    27181 atgattgccc tgcaccttcg cgatgcgttc atcgcgctcg cactcccagt cggtaaccgg
    27241 atacatcttg ttccatgcgt tgaacagctg cgtttgctgg cgagagagtg tcaggttgta
    27301 ttggtcacgc atatagaagt aggtgcgcgc aatggcaccg cgtgcacgcg ctggtggttc
    27361 ggcagctttt tctttgaaat cgaccttcat ggcgcattga ccgtactggc cttcgccgcc
    27421 attccactgg ctgtacataa agttgccgcg atcgccattc acctcgccga ctgacggctg
    27481 caggttatgc atatcgcttt ccatcttgcg atagaccgga tctttagcgc agtttttacg
    27541 tccaccgtcc tgccagcact ggcgctggtg accgaattgc caggcgggaa cgacgtgttc
    27601 ccactctacg cggctggcgc ggttttcatt tttgcgcacc tgatagccgc acgattgcag
    27661 atcaacaacg ccttttttgc cctgccagtt aattttacat ccgcaataaa acgtaccggg
    27721 cgcgtcagcg tggactttta ccgccgcggc tttcgcctga gaaaaactat tgataccttc
    27781 ggccaacgcc gggccggaaa atgctgcgct cagtaccacc gcagcaatag acaaataacg
    27841 gtacatcact tactccgtgt taaaacgagc cagcaacgta gcgaaagttg ttttggtatg
    27901 caatcagcca gatcaggaaa gttcctgata gttcagatgg ttattttgcg accagttgtt
    27961 cgccgcagtg aacacagcga tagacggctt cgccacgaac tacgcgatta tggcggcgta
    28021 cggtaagctg atgctcctgg cacttgcagc ggtaggggaa ggtgttgcga cgcacggatt
    28081 gcagttcgaa ctgatgcgta cgacgggctg gaacgcccag cacactttcc atcatccact
    28141 tccactcttt gccatgtggt gcaacgcggc caaaatgttt ccataccagc aaatgtgcca
    28201 gttcgtgcgg caccacttct tcaataaagg cttcactgtt ttccaacagc aaaacgggat
    28261 tgaggcggat ttcatagctt tccagccagg ctgttccggc ggaggttccg cgctgggtgt
    28321 aagagagttt tggctccggg tagttacgcc ctagcttcag gttggcctgg gcgagttttt
    28381 cccgcaggcg acgcataacg gcctgttgga tggcgatagg gagacgggat gttttcatag
    28441 cggcagagga tagagcgaag aaaagatgac tgcaagaggc ggcttcctcc gcgacgggag
    28501 gaagcctgag gagattaatc gtgagcgccg atttcgcgca gtttacgacc tttcatcaga
    28561 ttacgttcaa tatgttccag cgaaacgtgt ttggtttccg gtaccagcca caatgtcagc
    28621 aggataaaca gtacgttcag agccgcatac acccagaagg tgttggcgtt acccagcgtg
    28681 ttgagcatgg tcaggaacgt tgcgccaacg atcatgttgg caatccagtt ggtggcggtg
    28741 gagcaggtga tgccaaaatc gcggcctttc agcggctgaa tttcggagca cagtacccaa
    28801 atcagcggac cggcactcat ggcaaaaccg acaataaaca tcagcagcat ggcgatggcg
    28861 aaatactgcg ccgacggaga gtggataccg atatgcatca ttgtaccgag tacgcccatg
    28921 ccagcagcca tcaccaggaa gcccagcgtt agcgttggtt tacgtcccca gcggtcaaca
    28981 agaccgattg cgataaaggt ggcaagtacg ttggtcaggc cgacaatcac tgtcccccac
    29041 atttgctcgg tagtgttggt ataacccgcc agttcgaaga ttttcggcgc gtaatacatg
    29101 atgacgttca tcccggtgaa ttgctgcatt acctgtaaca gtacgccaag gaacaccgcg
    29161 cggcggaagt tgctattctc tttaaacagc gcccagccac tctgtttaac ctgcaaactt
    29221 tcacggattt catccagttc gcgtttcgct tccgcgctgg tgtcacgcag gcgtagcagc
    29281 acgcgttcgg catcaacaaa acggcgtttg gcggcaaacc aacgtgggct gtctggcagg
    29341 aagaagacac caatcagcag caaaattgcc gggatgataa tcacacccag catccagcgc
    29401 catgcaccgg tgtagctgaa ggcagtatca gaaagataag caccgaggat cccgatagtg
    29461 atcatcaact gatacatcga gatcatactg ccgcgaattt tttccggcgc aatttcagag
    29521 aggtacagtg gtgcggtata agaggccaca cctaccgcca gccccagcag aacgcgggaa
    29581 agaatcagta cttcaacgtt tggcgcagcc gcagagaaca gcgaaccggc aacaaacaaa
    29641 attgcgccga tcatcaggct ctttttgcgc ccgagtttaa aggagagcca gccgctgccc
    29701 accgcaccga ctgccgcacc gaacatcatg gagcttacga cccattcttg cgtgtgcgaa
    29761 gtaatctgga attcatctgc aataaacggc agtgcgccag caattacacc gatatccagg
    29821 ccaaagagta atcccgccag agcggcaagg aagcagacga aaaacgtcat tgccttgttt
    29881 gaccgcccct gttttttagc gtcaggcatg atgccctcca atatggttat tttttattgt
    29941 gaattaagat aggtgagtac gacgtaaaaa gatgtgaagc aaatcacatc agtgtaatcg
    30001 cttacactaa catttatctt agttatgatt taagtgcatg aaaataattg agttatatca
    30061 tcatagatta aacgctgtta tctgcaatta agactttact gaaaagaaat gtaacaactg
    30121 tgaaaaccga ataataatat gaaaatatta caaatctttg tctgttgtgg ttggtgtaat
    30181 cggtttcagt tgtgatgata atttgcggtg aaacgtgatc aggaagaatg atgttattgt
    30241 gcaggcggtt tttttgccgg atgcggcgtg aacgcctatc caccgcggtg aggtaaaaga
    30301 aaaaggccag cgcgaggctg gcctttgaaa gcaggtgaag aaagattact tcagaccggc
    30361 agcatcgcgc agcagctgcg ctttgtcggt tttttcccac gggaaatgtt cacgaccaaa
    30421 gtgaccgtat gctgcggttt ctttgtagat cgggtgcagc agatccagca tctgaatcag
    30481 accgtatggg cgcaggtcga agaactcacg taccagcaga gtcagttgtt cagaaggcac
    30541 tttctcggta ccgaaagttt ctaccatgat ggaagtcggt tcagccacgc cgattgcgta
    30601 ggaaacctga atttcacaac gatcggctag gccagcagca acgatgtttt ttgcaacata
    30661 acgtgctgcg tacgcagcgg aacggtccac ttttgatgga tctttaccag agaatgctcc
    30721 gccaccgtga cgtgccatgc cgccgtaggt atcaacgata attttacgac cagtcagacc
    30781 gcagtcaccc atcgggccac cgataacaaa acgaccggtc gggttgatga agaatttggt
    30841 ggcagaagtc agccattcag cgggcagaat cggcttgatg atctcttcca ttaccgcttc
    30901 ttgcagcgat ttctggtcga tctcttcaga gtgctgagtg gaaagcacga cagcatcgat
    30961 accaacgatt ttgccgtcgt catactggaa agtcacctgg cttttcgcgt ccgggcgcag
    31021 ccacggcaga gtgccgtttt tacgcacttc agcctgacgc tgtaccagac ggtgtgcata
    31081 ggtgataggt gctggcatca gcacgtcggt ttcattagtt gcgtagccaa acatcagacc
    31141 ctggtcaccc gcgccctgtt ccagcggatc ggcacggtca acgccctggt tgatgtcagg
    31201 agactgtttg ccgatagcgc tcagaaccgc acaggagtta gcgtcaaagc ccatgtcgga
    31261 atgtacatag ccaatttcgc gaacggtgtt acgggtgatc tcttcgatgt ctacccaggc
    31321 gctggtggtg atttcgccgc caactaaaac catgccggtt tttacgtagg tttcgcaagc
    31381 aacgcgtgct ttcggatcct gttcgaggat cgcgtctaaa acggcatcag agatttggtc
    31441 agcaattttg tcaggatgcc cttcagagac ggactcggac gtaaaaaggt gttttgccat
    31501 atttaatatc acctaaagag aatttggtta gctcaaactg ttgtgtggat tttctgtggt
    31561 agcggatcct accacgactc tgcaggttaa aaacactggc agtctgagtg ttaatcggta
    31621 tggatggatt aacatctgga tggctatttt aggtcaattc ttcacccgat ttccactttt
    31681 ttttgaatcg tgtctcattc tgttaaaaac gtggctggaa atttttcctg acaatgccgg
    31741 cattctgcgt atttatcttt tgcaattttc tgccattgtg gggtataaaa cgcggcgcgc
    31801 ggcttaaata aaaagcacac gacgtttctt tcgtgttgcc acttccagcc gggttgaaat
    31861 cagagttttg gcttgtgggt tcgtcttaac aggcggccgt ggaggtgata cgaaataatg
    31921 aaccgttgtc tgctgcttaa cctgtctcac cgttctggtg aagattcgtt ccccgcactc
    31981 tgcatctctg ctttgcatac ctgccgatgt tatacccatc tcggcgcttc tcaggattca
    32041 agagctggtt acagttactg aggactgaac aagggcgctc ttgtaaaaac aagagttttc
    32101 tcgtggtttc gccgaactgt cacacttacg ttcggttatg tgcttaataa tgttatgaaa
    32161 aagaaaccgg ttgcgcagtt ggagcgtcag cattcactgc tggaaaatcc atgtgcttat
    32221 gggttgttat cgcagttcca ggctgcgata gtcgttaact gttttacact taataaaata
    32281 atttgaggtt cgctatgtct gacgacatgt ctatgggttt gccttcgtca gcgggcgaac
    32341 acggtgtact acgctccatg caggaggttg caatgagctc ccaggaagcc agcaagatgc
    32401 tgcgtactta caatattgcc tggtggggca ataactacta tgacgttaac gagctgggcc
    32461 acatcagcgt gtgcccggac ccggacgtcc cggaagctcg cgtcgatctc gcgcagttag
    32521 tgaaaactcg tgaagcacag ggccagcgtc tgcctgcact gttctgtttc ccacagatcc
    32581 tgcagcaccg tttgcgttcc attaacgccg cgttcaaacg tgcgcgggaa tcctacggct
    32641 ataacggcga ttacttcctt gtttatccga tcaaagttaa ccagcaccgt cgcgtgattg
    32701 agtccctgat tcattcgggc gaaccgctgg gtctggaagc cggttccaaa gccgagttga
    32761 tggcagtgct ggcacatgct ggcatgaccc gtagcgtcat cgtctgcaac ggttataaag
    32821 accgcgaata tatccgcctg gcattaattg gcgagaagat ggggcacaag gtctatctgg
    32881 tcattgagaa gatgtcagaa atcgccattg tgctggatga agcagaacgt ctgaatgtcg
    32941 ttcctcgtct gggcgtgcgt gcacgtctgg cttcgcaggg ctcgggtaaa tggcagtctt
    33001 ccggcgggga aaaatcgaag ttcggcctgg ctgcgactca ggtactgcaa ctggttgaaa
    33061 ccctgcgtga agccgggcgt ctcgacagcc tgcaactact gcacttccac ctcggttcgc
    33121 agatggcgaa tattcgcgat atcgccacgg gcgttcgtga atccgcgcgt ttctatgtgg
    33181 aactgcacaa gctgggcgtc aatattcagt gcttcgacgt cggcggcggt ctgggcgtgg
    33241 actatgaagg cacccgttcg cagtctgact gctccgtaaa ctatggcctc aatgaatacg
    33301 ccaacaacat catctgggcg attggtgatg cgtgtgaaga aaacggtctg ccgcatccga
    33361 cggtaatcac tgaatcgggt cgtgcagtga ctgcgcatca caccgtgctg gtgtctaata
    33421 tcatcggcgt ggaacgtaac gaatacacgg tgccgaccgc gcctgcagaa gatgcgccgc
    33481 gcgcgctgca aagcatgtgg gaaacctggc aggagatgca cgaaccggga actcgccgtt
    33541 ctctgcgtga atggttacac gacagtcaga tggatctgca cgacattcat atcggctact
    33601 cttccggcac ctttagcctg caagaacgtg catgggctga gcagctttat ttgagcatgt
    33661 gccatgaagt gcaaaagcag ctggatccgc aaaaccgtgc tcaccgtccg attatcgacg
    33721 agctgcagga acgtatggcg gacaaaatgt acgtcaactt ctcgctgttc cagtcgatgc
    33781 cggatgcgtg ggggatcgac cagttgttcc cggttctgcc gctggaaggg ctggatcaag
    33841 tgccggaacg ccgcgctgtg ctgctggata ttacctgtga ctctgacggt gctatcgacc
    33901 actatatcga tggtgatggt attgccacga caatgcctat gccggagtac gatccagaga
    33961 atccgccgat gctcggtttc tttatggtcg gcgcatatca ggagatcctc ggcaacatgc
    34021 acaacctgtt cggtgatacc gaagcggttg acgtgttcgt cttccctgac ggtagtgtag
    34081 aagtggaact gtctgacgaa ggcgataccg tggcggacat gctgcaatat gtacagctcg
    34141 atccgaaaac gctgctaact cagttccgtg atcaagtgaa gaaaaccgat cttgatgctg
    34201 aactgcaaca acagttcctt gaagagttcg aggcaggttt gtacggttat acctatcttg
    34261 aagatgaata attaagatta ttccgctggc gggtcgattt atttacgtaa atcggcccgc
    34321 tttcttttta tatattctta aaaataattt agctgctttt cttattaaat aatctcccta
    34381 caatcgctgc attaattaaa tgcagaggga acggttatga aaaaatggaa agtacgtagc
    34441 gcgttgatag cattagtcgt gctgctggtc ggttgttcca gtaatgcgca gtataatagc
    34501 agtgcatcgg gcaatgtcgg caccgcctgg ggtggggatg tgcactcatc ggttcagggc
    34561 gtatctgctg aacgagcctg gcgcgatcct gcagagatga tcgttattag ttattcgact
    34621 aatgttcctt ccggctacga ccgcgtttat agcattcgta ttaacgaact ggaatatgct
    34681 attcgcgatg gtaattttaa ttctctacct attacgcggg cttatgattc atccaataat
    34741 gaaccacgct atattgtcca cgccagagtc ggaatgaatt atcaattata tgttcgtaat
    34801 tacagtcgta acaccaacta cgagatcgtt gctacggttg atggcctgga tgtgctgaac
    34861 ggcaagcaag gttcgctgaa taacaacggc tatattgtga atgccggaga ttcgttagcg
    34921 attaaaggat tccgcaaaga taaacatacc gaagcggctt ttcagttcgc caacgttgtt
    34981 gattcctacg cagccaattc ggcgcagggc gatgtgcgta ataccggggt aatcggcttt
    35041 gccgcctttg aattacaagg cccggcgcaa aacgcgctgc caccgtgctc tggacaagct
    35101 tttccagcag ataacaatgg ctatgcgccg ccaccgtgtc ggaaatagca acctcgcttg
    35161 atgcctcgcc catttaaggc gataattagc acaaacttgt gtttcgcaaa tccaaccctt
    35221 cctcgtcggg cctaacgacg cggaagggtt tttttatatc gactttgtaa taggagtcca
    35281 tccatgagca ccttaggtca tcaatacgat aactcactgg tttccaacgc ctttggtttt
    35341 ttacgcctgc cgatgaactt ccagccgtat gacagcgatg ccgactgggt gatcaccggc
    35401 gtgccgttcg acatggccac ctctggccgt gccggtggtc gtcacggccc ggcggcgatt
    35461 cgccaggttt cgactaacct ggcctgggaa cacaaccgct tcccgtggaa tttcgacatg
    35521 cgcgagcgtc tgaacgtggt ggactgcggc gatctggtat atgccttcgg cgatgcccgt
    35581 gagatgagcg aaaaactgca ggcacacgcc gagaagctgc tggctgccgg taagcgtatg
    35641 ctctccttcg gtggtgacca ctttgttacg ctgccgctgc tgcgtgctca tgcgaagcat
    35701 ttcggcaaaa tggcgctggt acactttgac gcccacaccg atacctacgc gaacggttgt
    35761 gaatttgacc acggcaccat gttctatacc gcgccgaaag aaggtctgat cgacccgaat
    35821 cattccgtgc agattggtat tcgtaccgaa tttgataaag acaacggctt taccgtgctg
    35881 gacgcctgcc aggtgaacga tcgcagcgtg gatgacgtta tcgcccaggt gaaacaaatt
    35941 gtgggtgata tgccggttta cctgaccttt gatatcgact gcctggatcc tgcatttgcg
    36001 ccgggcaccg gtaccccggt aattggcggc ctgacctccg atcgcgctat taaactggta
    36061 cgcggcctga aagatctcaa catcgttggg atggacgtag tggaagtggc tccggcatac
    36121 gatcagtcgg aaatcactgc tctggcagcg gcaacgctgg cgctggaaat gctgtatatt
    36181 caggcggcga aaaagggcga gtaagcacca gatgcgatgc gcacgggtaa aacgtgccat
    36241 tcatgtcaga tgcggggtga acgccttatc tgacctacgt ttggcacccg taggccggat
    36301 aagatgcgcc agcatcgcat ccggcaatgc gcacgaggta gtgaatgtgc catttatgtc
    36361 agatgcggcg taaatgcctt atctgaacta cgtttggcac caccatgcct tacttaatcc
    36421 cgtccgcgct catacgatcg cgaatatgct gggcgcgttc ggcggatgct ggatggtcgt
    36481 caaacattga gctttggcga ccttcttcca gttttgccag tttttcaaag ctggtggcaa
    36541 gacctgccgg actgatgccg cgttggcgca gtaaatcgta ggagtaatca tccgcctctg
    36601 cttcctggcg ctgggagaat tgcgaattga ctaatttctc gcccagatca ccaagctgtg
    36661 attgcgataa actgccgacg ataccgcccg cagaggccgc cgcgactcgc acggcatttg
    36721 taccaagtgc cacctgcatc cctttcttca catggcctaa cgccacgtgc cccatttcgt
    36781 gaccgatcac cgcttcgact tcgttatccg tcatcatatc catcagcccg ctatagacgc
    36841 ggatacagcc gttagccatc gcaaaggcgt tcacatcctt cgccatatac actttgtaat
    36901 tcactggctg accgttgata ttgttgccta gcgcgttggc aatggttgtc agacgtttag
    36961 cgtattcgct attggctggc gcaatcgtcg ccttgctgtc catctcctga catgcctgat
    37021 cgctcagggt tttcacctga gcatcactca aactgtaagc ctgaaaagct tccgctcctg
    37081 atgagagcag tccgttggag tccatattct ggcaaccagt cagtaccgtt gccacgctca
    37141 ttgctaccaa taaggcgcga attttcatgt tttttcttcc gcacaaggtc aggattattc
    37201 taaaaatgcg ccgtttgcag gtgaaccgac gctcagtctc agtataagga aaagcgcagc
    37261 ggacgggcga gtagattgcg caacatgcga gcatgatcca gagatttctg aagcggcaaa
    37321 aggatgttcc atgtacatga cgcgcggctt gcggtaaatt gttggcaaat tttccggcgt
    37381 agcccaaaac gcgctgtcgt caagtcgtta agggcgtgcc cttcatcatc cgatctggag
    37441 tcaaaatgtc ctcacgtaaa gagcttgcca atgctattcg tgcgctgagc atggacgcag
    37501 tacagaaagc caaatccggt cacccgggtg cccctatggg tatggctgac attgccgaag
    37561 tcctgtggcg tgatttcctg aaacacaacc cgcagaatcc gtcctgggct gaccgtgacc
    37621 gcttcgtgct gtccaacggc cacggctcca tgctgatcta cagcctgctg cacctcaccg
    37681 gttacgatct gccgatggaa gaactgaaaa acttccgtca gctgcactct aaaactccgg
    37741 gccacccgga agtgggttac accgctggtg tggaaaccac caccggtccg ctggggcagg
    37801 gtattgccaa cgcagtaggt atggcgattg cagaaaaaac gctggcggcg cagtttaacc
    37861 gtccgggcca cgacattgtc gatcactaca cctacgcctt catgggcgac ggctgcatga
    37921 tggaaggcat ctcccacgaa gtttgctctc tggcgggtac gctgaagctg ggtaaactga
    37981 ttgcgttcta cgatgacaac ggtatctcta ttgatggtca cgttgaaggc tggttcaccg
    38041 acgacaccgc aatgcgtttc gaagcttacg gctggcacgt tattcgcgac atcgacggtc
    38101 atgacgcagc atccatcaaa cgcgcagtag aagaagcgcg cgcagtgact gacaaaccgt
    38161 ccctgctgat gtgcaaaacc atcatcggtt tcggttcccc gaacaaagcc ggtactcacg
    38221 actcccacgg tgcgccgctg ggcgacgctg aaattgccct gacccgcgaa cagctgggct
    38281 ggaaatacgc gccgttcgaa atcccgtctg aaatctatgc tcagtgggat gcgaaagaag
    38341 caggtcaggc gaaagaatcc gcatggaacg agaaattcgc agcttacgcg aaagcttatc
    38401 cgcaggaagc cgctgaattt acccgccgta tgaaaggcga aatgccgtct gacttcgacg
    38461 ccaaagcgaa agagttcatt gctaaactgc aggctaaccc ggcgaaaatc gccagccgta
    38521 aagcgtcgca gaatgctatc gaagcgttcg gcccgctgtt gccggaattc ctcggcggct
    38581 ccgctgacct ggcaccgtct aacctgaccc tgtggtctgg ttctaaagca atcaacgaag
    38641 atgctgcggg taactacatc cactacggtg ttcgcgagtt cggtatgacc gcgattgcta
    38701 acggtatctc cctgcacggt ggcttcctgc cgtacacctc caccttcctg atgttcgtag
    38761 aatacgcacg taatgccgta cgtatggctg cgctgatgaa acagcgtcag gtgatggttt
    38821 acacccacga ctccatcggt ctgggcgaag atggcccgac tcaccagccg gtagagcagg
    38881 ttgcttctct gcgcgtgacc ccgaacatgt ctacatggcg tccatgtgac caggttgaat
    38941 ctgcagtggc atggaaatac ggtgttgagc gtcaggacgg cccgaccgcg cttatcctct
    39001 cccgtcagaa cctggcgcag caggagcgta ctgaagagca gctggcaaac atcgcgcgcg
    39061 gtggttatgt gctgaaagac tgtgccggtc agccggaact gatcttcatc gcgaccggtt
    39121 cggaagttga actggctgtt gccgcctacg aaaaactgac tgccgaaggc gtgaaggcgc
    39181 gcgtggtttc catgccgtct accgatgcat tcgacaagca ggatgcggct taccgtgaat
    39241 ccgtactgcc gaaagcggtt actgcacgcg ttgcggtaga agcgggtatt gctgactact
    39301 ggtacaagta tgttggcctg aacggtgcta tcgtcggtat gaccaccttc ggtgaatctg
    39361 ctccggcaga gctgctgttt gaagagttcg gcttcactgt tgataacgtt gttgcgaaag
    39421 caaaagcgct gctgtaatca gtaagacgtt ttgtgagaag ggccgcgaaa gcggcccttt
    39481 ttttacttag tagagataaa cggaactgtg ctgccgggga tcatggtatc gggcagttta
    39541 ccgtcccaac gttcggcagt ggtgagggct acaagtccgg gattatcccg cagagcttca
    39601 ccgcgcaggc ggattgcttc tgcttcagcc gcacttttta aacgaatagt ttcagcctct
    39661 gccgcgcctc ttacgcgaat agtttccgct tctgctttgg ctgccgccag tttactgtct
    39721 gcttctgcct gggcctgagt taccgctatt tgcgcctgga ttttctctgt ttccagattt
    39781 tgcttacgcg tggcaatggc gacttctgct ttcattcggt cttcaatcga cttttcataa
    39841 gcgtcagaaa aatcaatatt ttcaatctgg accccgtcaa ttaccactgg cccaacaacg
    39901 gctttgcgca tggcattttg cagatcctga accagttttg tacgatcctg tacagcgctg
    39961 atggcggtat attggccgaa aacgttctcc agttgagttg gcaactgacg gacaatcaga
    40021 cgatctttta atgcttcaat ggtgttgtag gtggtataaa ccgcgcctgc ttctgaaggt
    40081 ttaatgtgga agctgactga tactgtcatt tgtgccggtt gctggtcgcg gctgtaggcc
    40141 tgtaatccct gatagaccac agcctgatta cgcgtggaaa ttttctccac gctttccata
    40201 aacgggatct taaaacccag acccggctcg gcaactttta cgatttttcc gtagcgcagc
    40261 aaaatgccgc gttcaccttc attaacggtg taatacgaga gaaatggcag caaaaccacg
    40321 gcaagcacgc cgatggcgat agcaagagat ttttgtgggc gaaatgaggt taatgaaaca
    40381 ggtgctttca tgacaatctt ccttgagtaa agtgtgggaa tatgtcacat gagatgagga
    40441 gaaaacagcc gacaggtgag aattcagatt ctataaaggg gtatagcgat aaaacattgc
    40501 attagaaaag tatgttttcg aacattcaat aaat
//
LOCUS       DAZGCQ010000039        40153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000039 DAZGCQ010000000
VERSION     DAZGCQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.039"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..311
                     /locus_tag="ACHK07_002170"
     CDS             <1..311
                     /locus_tag="ACHK07_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102497.1"
                     /translation="FKYIQAEAKQDFKDQYKLTKNDYLKKIPLTAQLIAKYKMDDQLD
                     QLLVTREIQDEIKSKIQDKIDELSKNLFNTMTETIENNFDDIFRQQSENMSNYYEFVD
                     "
     gene            complement(489..1004)
                     /gene="pgpA"
                     /locus_tag="ACHK07_002171"
     CDS             complement(489..1004)
                     /gene="pgpA"
                     /locus_tag="ACHK07_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO8102498.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(982..1959)
                     /gene="thiL"
                     /locus_tag="ACHK07_002172"
     CDS             complement(982..1959)
                     /gene="thiL"
                     /locus_tag="ACHK07_002172"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO8102499.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(2037..2456)
                     /gene="nusB"
                     /locus_tag="ACHK07_002173"
     CDS             complement(2037..2456)
                     /gene="nusB"
                     /locus_tag="ACHK07_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO8102500.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2476..2946)
                     /gene="ribE"
                     /locus_tag="ACHK07_002174"
     CDS             complement(2476..2946)
                     /gene="ribE"
                     /locus_tag="ACHK07_002174"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO8102501.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(3035..4138)
                     /gene="ribD"
                     /locus_tag="ACHK07_002175"
     CDS             complement(3035..4138)
                     /gene="ribD"
                     /locus_tag="ACHK07_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO8102502.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4142..4591)
                     /gene="nrdR"
                     /locus_tag="ACHK07_002176"
     CDS             complement(4142..4591)
                     /gene="nrdR"
                     /locus_tag="ACHK07_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO8102503.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGVLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4742..5281
                     /gene="yajI"
                     /locus_tag="ACHK07_002177"
     CDS             4742..5281
                     /gene="yajI"
                     /locus_tag="ACHK07_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8102504.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHNVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMH"
     gene            5580..6464
                     /gene="tsx"
                     /locus_tag="ACHK07_002178"
     CDS             5580..6464
                     /gene="tsx"
                     /locus_tag="ACHK07_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO8102505.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6654..7001)
                     /gene="yajD"
                     /locus_tag="ACHK07_002179"
     CDS             complement(6654..7001)
                     /gene="yajD"
                     /locus_tag="ACHK07_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO8102506.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7130..8101)
                     /gene="secF"
                     /locus_tag="ACHK07_002180"
     CDS             complement(7130..8101)
                     /gene="secF"
                     /locus_tag="ACHK07_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO8102507.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8112..9959)
                     /gene="secD"
                     /locus_tag="ACHK07_002181"
     CDS             complement(8112..9959)
                     /gene="secD"
                     /locus_tag="ACHK07_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO8102508.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9987..10319)
                     /gene="yajC"
                     /locus_tag="ACHK07_002182"
     CDS             complement(9987..10319)
                     /gene="yajC"
                     /locus_tag="ACHK07_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO8102509.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10342..11469)
                     /gene="tgt"
                     /locus_tag="ACHK07_002183"
     CDS             complement(10342..11469)
                     /gene="tgt"
                     /locus_tag="ACHK07_002183"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO8102510.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11524..12594)
                     /gene="queA"
                     /locus_tag="ACHK07_002184"
     CDS             complement(11524..12594)
                     /gene="queA"
                     /locus_tag="ACHK07_002184"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO8102511.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12687..13268
                     /gene="acpH"
                     /locus_tag="ACHK07_002185"
     CDS             12687..13268
                     /gene="acpH"
                     /locus_tag="ACHK07_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO8102512.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVITILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(13289..14770)
                     /locus_tag="ACHK07_002186"
     CDS             complement(13289..14770)
                     /locus_tag="ACHK07_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102513.1"
                     /translation="MTISNSNLLKPFITGAELPPFKEDVFSKTEEQFKKSTHGKSLFI
                     CGNENLVNISAEKIDEIKNSKNFFSNVVNIIILLIKYPDKILPILHGCPAESIELIKN
                     AKRTGQWSYEMTDKNGQNIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIAKAGFITGKVNAEKFNVKEF
                     KDDITSMLCEQPPMCTHQISSSGYLSADKVPDLYKTNLKDAQRDLKTTPVDIDGVSLK
                     SKTREKIAEEIAKKTAEKTAEKTAKKNSEIIAKQNANSIEEKNIEKNNKETNPEDNFV
                     LKFSQEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQCLYNVARDGSPRYAGLL
                     QQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNG
                     ELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
     gene            complement(14958..16775)
                     /gene="malZ"
                     /locus_tag="ACHK07_002187"
     CDS             complement(14958..16775)
                     /gene="malZ"
                     /locus_tag="ACHK07_002187"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO8102514.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGSQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(16931..18304)
                     /gene="proY"
                     /locus_tag="ACHK07_002188"
     CDS             complement(16931..18304)
                     /gene="proY"
                     /locus_tag="ACHK07_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO8102515.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(18380..19699)
                     /gene="brnQ"
                     /locus_tag="ACHK07_002189"
     CDS             complement(18380..19699)
                     /gene="brnQ"
                     /locus_tag="ACHK07_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO8102516.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(20106..21401)
                     /gene="phoR"
                     /locus_tag="ACHK07_002190"
     CDS             complement(20106..21401)
                     /gene="phoR"
                     /locus_tag="ACHK07_002190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO8102517.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(21459..22148)
                     /gene="phoB"
                     /locus_tag="ACHK07_002191"
     CDS             complement(21459..22148)
                     /gene="phoB"
                     /locus_tag="ACHK07_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO8102518.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            22338..23540
                     /gene="sbcD"
                     /locus_tag="ACHK07_002192"
     CDS             22338..23540
                     /gene="sbcD"
                     /locus_tag="ACHK07_002192"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO8102519.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            23537..26680
                     /gene="sbcC"
                     /locus_tag="ACHK07_002193"
     CDS             23537..26680
                     /gene="sbcC"
                     /locus_tag="ACHK07_002193"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO8102520.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTVPQEDEEESWLATRQQEAQSWQQHQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            26806..27990
                     /gene="araJ"
                     /locus_tag="ACHK07_002194"
     CDS             26806..27990
                     /gene="araJ"
                     /locus_tag="ACHK07_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO8102521.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(28124..29032)
                     /gene="mak"
                     /locus_tag="ACHK07_002195"
     CDS             complement(28124..29032)
                     /gene="mak"
                     /locus_tag="ACHK07_002195"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO8102522.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            29157..30068
                     /gene="rdgC"
                     /locus_tag="ACHK07_002196"
     CDS             29157..30068
                     /gene="rdgC"
                     /locus_tag="ACHK07_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO8102523.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(30146..30601)
                     /locus_tag="ACHK07_002197"
     CDS             complement(30146..30601)
                     /locus_tag="ACHK07_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102524.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(30598..32877)
                     /locus_tag="ACHK07_002198"
     CDS             complement(30598..32877)
                     /locus_tag="ACHK07_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO8102525.1"
                     /translation="MNRINILVICMVLFFMTGYACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLNVRKTLLSHYDYAWLVPTAVAD
                     ELINSEDPELRERITGADLTAQQAVLLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKALLIALPEMRQLSLAKEDVHNLREGTRYLTSKEVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSLLRDLVK
                     TGATRKIRSEARHRLEEKQ"
     gene            complement(33021..33305)
                     /gene="ppnP"
                     /locus_tag="ACHK07_002199"
     CDS             complement(33021..33305)
                     /gene="ppnP"
                     /locus_tag="ACHK07_002199"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO8102526.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(33377..34054)
                     /gene="aroM"
                     /locus_tag="ACHK07_002200"
     CDS             complement(33377..34054)
                     /gene="aroM"
                     /locus_tag="ACHK07_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO8102527.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(34312..34503)
                     /gene="yaiA"
                     /locus_tag="ACHK07_002201"
     CDS             complement(34312..34503)
                     /gene="yaiA"
                     /locus_tag="ACHK07_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO8102528.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(34553..35077)
                     /gene="aroL"
                     /locus_tag="ACHK07_002202"
     CDS             complement(34553..35077)
                     /gene="aroL"
                     /locus_tag="ACHK07_002202"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO8102529.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(35260..35718)
                     /gene="yaiI"
                     /locus_tag="ACHK07_002203"
     CDS             complement(35260..35718)
                     /gene="yaiI"
                     /locus_tag="ACHK07_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO8102530.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            35838..36647
                     /gene="proC"
                     /locus_tag="ACHK07_002204"
     CDS             35838..36647
                     /gene="proC"
                     /locus_tag="ACHK07_002204"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO8102531.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRSQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(36664..37779)
                     /gene="adrA"
                     /locus_tag="ACHK07_002205"
                     /gene_synonym="dgcC"
     CDS             complement(36664..37779)
                     /gene="adrA"
                     /locus_tag="ACHK07_002205"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO8102532.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(37881..38201)
                     /gene="psiF"
                     /locus_tag="ACHK07_002206"
     CDS             complement(37881..38201)
                     /gene="psiF"
                     /locus_tag="ACHK07_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO8102533.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(38320..39735)
                     /gene="phoA"
                     /locus_tag="ACHK07_002207"
     CDS             complement(38320..39735)
                     /gene="phoA"
                     /locus_tag="ACHK07_002207"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO8102534.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFNGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(39836..40096)
                     /gene="iraP"
                     /locus_tag="ACHK07_002208"
     CDS             complement(39836..40096)
                     /gene="iraP"
                     /locus_tag="ACHK07_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO8102535.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        10116 a        10584 c         9681 g         9772 t
ORIGIN      
        1 tttttaaata tatacaagca gaagccaaac aagatttcaa agaccagtat aaactaacaa
       61 aaaatgatta tttgaagaaa atacctttaa ctgcacaatt aattgcaaaa tacaaaatgg
      121 atgatcaatt ggaccaactt ttggttaccc gtgaaattca agatgaaata aaatcaaaga
      181 ttcaagacaa aatagatgaa ctttctaaaa atttattcaa cactatgacc gaaaccattg
      241 agaataattt tgatgatatt tttcgtcaac agtcagagaa tatgagcaat tattatgagt
      301 tcgtcgactg acgatatttc agtgtcattg aattatgagt gacaaagata caccttgaaa
      361 tatcgccgca tagtatattc ggcgatgtcg ggttttactt taacagagtg ccccaaaatt
      421 ggggcactgt tcatttataa atcaaaccgg gtggtctgga atcgcctaca cctcccggtc
      481 aatggcattt aaagaatacc cagcggccag tgatgaccga taaagtacag gatgcctgcg
      541 gaaatcaccc cggcgacaat atcatcgatc atgatcccca tgccgccatg tacattgcga
      601 tcaaaccagc ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaacccg
      661 gcggcgaccc actgccagtc attggtcggc agcgccatca gggtgatcca catgccaata
      721 aactcatccc agacgatgct accgtgatcg tgcacaccca tgtctttcgc cgtttgatga
      781 caaagataga cgccgataca gatccccaac atcaccacca gcgagtagag ctgccagggc
      841 aaaaaggtca tcagatacca gaacggaatc gccgccagcg atcccatcgt gccaggaacg
      901 attgggctta atccacttcc gaatccgaca gcaagcagat gccacggatt actcatcttc
      961 aggcgacttt tcgcgacatc tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa
     1021 cgtgacaggt tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc
     1081 gatacaggta aacggtacgc ccagatgacc gagagccaca tccagcgcgc cacggttcag
     1141 ttccggcacc gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc
     1201 ctgttccggt acaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat
     1261 acgtgcgccg cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga
     1321 gagatcgata gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga
     1381 tggacggaga tgacgtttga tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg
     1441 gttttgcaaa atcgccagcc cggcggcgct atcgcccggt gtaccggtca catagatcca
     1501 gtcacccggt ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat
     1561 acccaacgtc attgataatg gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta
     1621 ataattgaga agatcaaaca aactgtcgct gaaggactca agccacgctt cgtctacgtc
     1681 cggtaaggtt aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct
     1741 taggttcacc gccagtgctt tataagccag atcagcagga tcgatatcag ggaggaaatg
     1801 gttgcccgcc accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag
     1861 aagagcgcaa tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc
     1921 aaaataacgg gcaatcaggg agaactcgcc acatgccata cgttatgcct cagcaggtaa
     1981 aaaagaaaag gccggggatc gcggaacagt cttccgtgaa tctaccggcc tggatattac
     2041 tttttattag ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg
     2101 ctgtcttctg cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat
     2161 ggcacatcgc tacgtttaga cagttcgtac agcgcaatgc gcagtactgc tttttctacc
     2221 tgtcccagtt cttccagcag gcgggacagg tatggcttca tcagtccgtc gaggtatgcg
     2281 gtattagtcg ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca
     2341 tcctgttcag ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag
     2401 gagtagagcg cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat
     2461 ttccccttac taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt
     2521 cagtgcagct tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc
     2581 aatgctttca gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg
     2641 cgccaggccg ttgcttgcac caccagcgac atattcaaag tgggcagtgc caccacgaat
     2701 aaccgtaccc agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc
     2761 cagcggcagc tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg
     2821 accgatacgt ttcagtgcgt caattgcacc ttccagcagg ctgtcattga taaagttgtt
     2881 gaaacgcgcg atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat
     2941 gttcattttc ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat
     3001 atttcgtgcg ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa
     3061 catccgggcc tacatgacgt atctctttga atttaaattg gggggcgtcg gctaatttct
     3121 caagccctgg cagcgagcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat
     3181 agacaatcag ctcatcgact aaacccgcct gcagcaatgc gccagcgagc gttggccccg
     3241 cttccaccca gatgctgtta atctgctgtt tacccagttg catcatcagt acaaccagat
     3301 ccagatgacc tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag
     3361 aatcttcctg cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg tgatgttccg
     3421 gcgtcacgcg attttggcta tcaatcacaa tacgtaccgg ctggcggaga ttttgttgcg
     3481 ggtagagcgc ctgagtttgt tcatccagtt cggaccaacg caccgttaag gcaggatcat
     3541 ccgccagcac cgtggcgctg ctggttaaaa tggcatgact ttgcgcgcgc agtcgttgta
     3601 catcacgccg cgcctggggc gaagtgatcc actggctttc gccgctcgcc atcgccgtgc
     3661 gaccatcaag cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgtt
     3721 tgagaaagcc tttattcaat tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa
     3781 tgccagcctg ttgcagacgg taaagtccac gtccagcaac ctgcgggttc gggtcctgca
     3841 tcgcagcgac cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac
     3901 gaccatgatg gctacagggt tcgagtgtga cataggcggt cgcacctttg gctttttcac
     3961 ccgccatacg caacgcgtgt acttcagcat gtggttcacc cgctcgttgg tggtaacctt
     4021 caccgacaat ttcgccatct ttgacaatga cgcacccgac attcgggttg gggtgggtgg
     4081 taaaacgtcc tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg
     4141 cttagtcctc caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt
     4201 agacagaggc aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca
     4261 gattgccaat catcttgctc ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa
     4321 tatgattgat tgccatttcg acgtcatcgg aactcaccgg acgtttttcc agcgcccgca
     4381 gcactccgct acgtaatttt tcttcattaa acggttcacg cacgtcgttg ctttttacaa
     4441 cacgcggcat aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac
     4501 actgccgacg gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag
     4561 tgtccacggc gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacagaa
     4621 atctatttta ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc
     4681 gatgctttcg ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc
     4741 tatgaatact aacgtttttc gactgctcct gctgggaagc ctgttcagcc ttagcgcctg
     4801 tgtgcagcaa agcgaagtgc gacagatgaa acacaacgtc agcacgctga accaggagat
     4861 gacgcaactc aatcaagaaa cagtcaaaat cactcagcaa aacaggctga atgcaaaatc
     4921 cagcagtggg gtttaccttc tgcctggagc gaaaacaccg gcaagactgg aaagccagat
     4981 cggtacttta cgtatgtcgc tggtgaatat tacgcctgat acagatggca ccaccctgac
     5041 gctacgtatt cagggcgagt cgaatgatcc cttgcccgca ttcagcggaa ccgttgaata
     5101 tgggcaaatt cagggaacaa tagacaactt tcaggaaatc aatgtgcaga atcaattgat
     5161 taatgcccct gccagcgtcc tcgcccccag cgatgttgat attccgttac agttaaaggg
     5221 gatttctgtg gatcagttag actttgtacg tatccacgac attcaacctg ttatgcatta
     5281 aacgtattgc ggggcaattt tgtgcgtccc gcaacatctt tccccgtcat tttgttactc
     5341 cgcttacatc acccagattg atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc
     5401 cctgcccgaa tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa
     5461 cgaaacatat ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt
     5521 agaaacgcaa tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata
     5581 tgaaaaaaac attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca
     5641 acgcagctga aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg
     5701 ttgtcggaag ctatcacacc cgttttggac cgcagatccg caacgatacc taccttgagt
     5761 acgaagcatt cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat
     5821 tcttcggcgg taactccgat gccaaaggta tctggaacca cggctctccg ctgtttatgg
     5881 aaatcgaacc acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt
     5941 tcaaagagtg gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc
     6001 gccagagcac ctggtacatg ggtctgggta ccgatatcga cactggcctg ccgatgagcc
     6061 tgtccatgaa cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg
     6121 agtgggacgg ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg
     6181 gtcagctgag ctacatcggc ttcaccaact tcgactgggg ttccgattta ggggatgaca
     6241 gcggtaacgc aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata
     6301 ttctggctct gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg
     6361 gtggtcagtg gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct
     6421 ctaccggctg gggtggttac ctggtagtag gttacaactt ctgattatga aaatgccggg
     6481 atttattccc gggatttatt cccggcattt cagattgtta acaacgtgcg ctttgttcat
     6541 gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc acgtaaatgc aatatattgc
     6601 tgaaataacg taggcctgat aagcgtagcg catcaggcaa ttttacgttt taatcacttc
     6661 ttcttgttca tcatcgcttt caggtcagcg aatgggttgt acttcgcttc accgacatct
     6721 ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc ggtatatttc
     6781 gaatgctcat gatcgtggca atagagacaa agcaattccc agttactgcc atcttccggg
     6841 ttattggtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt ggaataaaca
     6901 aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt ttcgcgatag
     6961 ccgctttcta accgcgcata gttttttggg atgatagcca tgaaaagtta cctggatgaa
     7021 atgtagggtt acgcgcagtt tcccaagatg cggggatttt cgcaatctcc atacatcaaa
     7081 aaaaatcccg atcttctgac cgggattttc aacatcaacg ggaacttgat taaggcagaa
     7141 ttgacggctg atccgcccct tctttttcca ctttctgctg caacatgtgt tcgcgcttca
     7201 tacccagttt cagagccaac gcggatgcca catagatgga agatgcagta ccgatggaaa
     7261 caccaatcag catggtcagc gagaagcctt ccagtaccgg gccaccgaag aggtacagca
     7321 tcaggataac catcaaggta gtaccggatg tgatcaaggt acgatgcagc gtctgggtca
     7381 aggacacgtt aaagatttcg taaggcgtac cgcgacgtat cttgcggaag ttttcacgaa
     7441 tacggtccga taccacgata ctgtcgttaa gcgagtaacc gataaccgac atcaacgatg
     7501 ccacaatggt caggtcaatc tcgatatgga ataacgacaa aatacccagc gtaatgataa
     7561 cgtcgtgcgc cagcgcaata accacccctg ccgccagtcg ccactcaaag cggaaaccta
     7621 cgtacacgag gatagacagc agcgctgcca tcaacgccat cgcaccggtt tgcgcaaggt
     7681 ctgcccccac gctcggaccg acgaactcaa tacgcttcac tgctgcattc tgattggtgg
     7741 attcgttaat caccttcaga acctggctgc ccagcacctg accgccggtt tcgccttcag
     7801 caggcggcat acggaccatg atgtcgtggc tgctaccaaa gttttgcagc attggctctt
     7861 caaaaccggc tttttgcaat gcatcacgca ttacgtcaat ttcagccggt ttttcgagcg
     7921 taatttcgat aaccgtacca ccggtgaaat ccagccccca gttaaagccg cgcacgccca
     7981 taataacgat agcagcgatt aacagcagac cagagatgcc gaaagcccag tagtcccagc
     8041 gcataaagtc atagacttta cggccgtggt ttagttgttc aacagtatat tcctgtgcca
     8101 catcgcactc ctcagattga cagcttcttg acgcgcttgc cgccatatag caggtttacg
     8161 atggcacgcg taccgacaat cgcggtaaac atcgacgtcg ccacaccgat accggtagta
     8221 atcgcgaacc ctttaattgc cccggtaccc actgcgtaca ggatgatgac tttaatcagc
     8281 gtggtgatgt tcgcatcgaa gatagaactg aatgcgccac gataaccttc atcaattgcc
     8341 tgttgaacag tacgtccgtt gctcaactct tctttaatac gttcgttgat cagtacgttc
     8401 gcatcgaccg ctaccgcaag ggttaagacg atacccgcga tacctggcat actcagtgtt
     8461 gcgcctggca acagcgacat aataccgacg atcaagatca agttggcaat cagagcactg
     8521 gtcgcaatca gaccaaactt cttatagaag atgatcatga acagaataga aaccagcaga
     8581 ccggcaaggc aagcctccag cccctgctca atgttctgca tacccagggt cgggccaatt
     8641 gtacgttctt caacaatctg aatcggcgcg atcaacgcac cggcacgcag cagcagtgac
     8701 agctgacggg cttcgttcgg gttgttgatg ccggtgatac ggaagctgtt accaagacga
     8761 gactggatgt tggcgatgtt aatcacctct tcctgtttca ccagaaccgc acgaccattt
     8821 gcatctttct taccgctgtc tttgtactcc acaaacaggg ttgccatcgg tttgccgatg
     8881 ttgtccttgg tgaagttaga cataatgttg ccaccagcgc tatcgagcga gatgttaacc
     8941 tgcggctggt tgtattcgtc ctggctggaa gtggagtcgg tgatatggtc accggtcaga
     9001 attacgcgtt tgtacagcac aaccggctga ccttcgcggg tctgtttcac ttcagagtcg
     9061 cccggtacgc gaccggatgc cgcagcggcc tgatcaacgt tggtgtttac cagacggaat
     9121 tccagcgttg ccgtcgcacc cagaatctct ttcgcacgcg cagtgtcctg aatacctggc
     9181 agttcaacaa cgatacggtc agcaccctga cgctgaacca ccggctccgc cacgccaagt
     9241 tggtttacac ggttacgcag gatattgatg ttctgctgca ccgcatattc acgcgcttca
     9301 ctcagacgag catcgctcat aaccgcacgc agctggttgc tgccctggct actaatcacc
     9361 aggtccggat ggcgcttgct cagatacgca atggcttcat cacgagcttt agcatcgcgg
     9421 aaagtgatgc tcaggccgta gttgttttct ttacgaacgg tggtatacgg gatgcctttt
     9481 tcgcgcaggt cgctgcgcag gctatcgata ttttgttcct ggagtttgcc aagcgcggtg
     9541 tccatatcca cttccatcag gaagtgaacg ccgccacgca ggtcaaggcc gagcttcatc
     9601 ggctcagcgt gaatagctgc cagccagcgc ggcgttgccg gggcaaggtt aagcgccacg
     9661 acgtatttgt cacccataac gcccattaat gcttcacgag cgcgcagctg ggtgtcagtg
     9721 gagtcgaagc gcgcaagaat agcgccctct tccagtgcca cagacttagc agttattttt
     9781 tcttcttgta acgttttctg gacctggatc agcgtttgct cactggcggc gactccgcgc
     9841 gcaccagtga tctgaacagc cggatcctca ccaaacaggt tgggaagcgc atacagcaga
     9901 ccgatgacaa tcaccacgat cagcatgacg tacttccaca aaggataacg gtttaacacg
     9961 gcaattccct tagggaaaaa ttttaattac agcgccttca tggtgccttt cggcaggacg
    10021 gcagctacga agtcacgttt aataactact tcagtggtgt cattcagcgc gatagcaatg
    10081 tagccgtttt ccgctacttt ggttacgcga ccgaccaggc caccgttcgt cagaacttca
    10141 tcacctttgg caatggagtc catcagcttt ttgtgttctt tggtgcgctt ctgctgtgga
    10201 cgcaggatca tgaaatagaa aatcagaccg aataccacca gcatcaaaat caaagacatc
    10261 gggctacctt gcgccggtgc acccgttgcc gctaccgcat cagaaataaa aaagctcatt
    10321 aaatttccct cattattaat attaatcaac gttcaaaggt ggtacttctc gcccctgacg
    10381 ctggtaaaaa tcagttacga agctctctaa tttaccctct tcaatagcct tgcgtaaacc
    10441 cgccatcaaa cgctggtagt aacgaaggtt atgaatggta ttgagtcgcg cgcctaatat
    10501 ttcgttgcaa cggtcaagat gatgcaagta agcgcgtgaa taattgcgac aggtgtagca
    10561 atcacactca ggatcgagtg ggccagtatc gctcttatac ttcgcattgc ggattttcac
    10621 cacgccatcg gtcacgaaca aatgaccatt tcgggcgttg cgggttggca ttacgcagtc
    10681 aaacatatcg ataccacgac gtacgccttc aaccaggtct tctggtttac caacgcccat
    10741 caggtaacgc ggtttgtctg ccggaatttg cgggcataca tgctccagaa tgcggtgcat
    10801 atctgctttc ggctcaccca cagccagacc gccgacagcg tagccatcaa aaccgatatc
    10861 taccagacct ttaacagaaa tatcacgtaa atcttcgtaa acgctgccct gaatgatacc
    10921 aaacagcgca tttttgtttc cgagactgtc aaaacgctca cggctacgct tcgcccaacg
    10981 cagagacatc tccatggagc gttttgcgta atcccagtca gcaggatacg gcgtacactc
    11041 atcaaagatc atgacgatat ccgaaccaag atcgtactga atctccatcg acttttccgg
    11101 gtcgaggaaa atcgggtcgc cgttgatcgg gttacggaag tgcacgccct gttcggtgat
    11161 tttacgaata tcgccaaggc tgaagacctg gaagccgccg gagtcggtga ggatcggtcc
    11221 cttccactgc ataaaatcgt gcagatcgcc gtgcagtttc atgatttcct ggcccgggcg
    11281 cagccacagg tggaaggtgt tgccgaggat aatttgcgcg ccagtggctt caacttcttc
    11341 tggcgtcatc ccttttacgg tgccgtaggt gccaacaggc ataaaacaag gcgtttccac
    11401 tacgccacga tcaaagacca ggcggccacg gcgtgcgcga ccgtcggtgg tgtccagttc
    11461 aaatttcatt ttttctccac tacgtcagaa aaacagtcca acgtttaaac cagcgccgcg
    11521 gaattactcc ccgacgcgct cattaattgc ctgcggattg tacgtgataa acatcgcatc
    11581 accgtaacta aaaaagcgat atttctcttc taccgccgct ttataggcat tcatggtgtg
    11641 ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc gactctggca agtggaagtt
    11701 tgtcaccagc gcatcgacca ctttgtactg gaagcccgga tagataaaga tttgggtatc
    11761 gtcaaagaac ggttcgatga gatcgttttt cgccgcctga gccgcgcttt ccagcgaacg
    11821 tactgaggta gtgccaaccg caatcacccg gttaccgcgc gctttcgccg ccagtaccgc
    11881 gtctaccaca tcctgcggta cttcagcgta ttccgagtgc atgatgtgat cttcaatggt
    11941 gtcgacgcgc accggctgga aggtgcccgc accaacgtgc agggtgacga acgccatctc
    12001 cacgcctttg gcgcgtaatt tttccagcaa aggttcgtca aaatgcagac cagcggtcgg
    12061 ggctgcaacc gcgcccggtt tttcgctata aacggtttga taaagttcgc ggtcagcgtc
    12121 ttcgtccgga cggtcgatat acggcggcag cggcatatgg ccgatgctgt tgagaatatc
    12181 cagtaccgag cgttcatcat taaattcgac ttcaaacagt gcgccgtggc gcgcggtcat
    12241 tgttgcgttg atactttcgt catcgcccag caacagttct gcgccaggtt ttggcgcttt
    12301 cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc atccgctcaa ccagcacttc
    12361 aatcttgccg ccgctggctt tacgcccaaa caggcgcgcc gggatcacgc gggtattatt
    12421 aaaaaccaga agatcgccgg ggttgagctt atcgagtaaa tcggtgaaag taccgtgcgt
    12481 cagcgcgccc gtcggcccgt ccagcgacag taaacgacag ctactgcgtt caggcatcgg
    12541 atagtgggca atcagggatt cgggcaattc aaaggagaaa tcggtaacgc gcatgacact
    12601 gactcagact aaaataagag gcgggtagtc tagtgccggg gcgctctccc tgcaaccttt
    12661 accccccttt ggatagaata taatcgatga attttttagc tcacctgcat ttagcccatc
    12721 tcgcggaaag ctcgctttcc ggcaatttgc tggctgattt cgtacgcgga aatcctgaag
    12781 aaagttttcc gcccgacgtc gtggctggca ttcatatgca tcgacgtatc gacgtattga
    12841 ctgacaatct gccggaagtc cgcgaagcac gggagtggtt tcgtagtgaa actcgccgcg
    12901 ttgcgcctat tacgctggat gtcatgtggg atcactttct ttcccgtcac tggtcgcagc
    12961 tgtcgccgga ctttccgcta caggaatttg tctgttatgc ccgcgaacaa gtgataacga
    13021 ttttgccgga ctcaccgcca cgctttatca acctgaacaa ctacttatgg tcagagcagt
    13081 ggctggtgcg ctatcgcgat atggatttca tccagaacgt gttaaatggc atggcaagcc
    13141 gtcgcccacg tctggatgcc ctgcgtgact cctggtacga tttaaacgcc cattatgacg
    13201 ccctcgaaac ccgcttctgg cagttttatc cgcggatgat ggcgcaggcg tcacgcaagg
    13261 cgctctgaaa cgtcaaactc cggcgtcact actttttagc attcgcatcc agcggggcgg
    13321 cataacaacg gtaagaaaaa cattcgctgg aatgattggc gatatcttta aagttgcttt
    13381 cagcaacccc ctcgaacatg agtaccgttc tggacaactc tccgttgtca tttatatgtg
    13441 gaaaatcaaa ttgtgtactg aatcgccata catctgtctt ttctggctcg ctgttcacag
    13501 agatacgtcg atggaagact cctcctgaaa acgtgcgcca gaacaattgt tgatcaaaat
    13561 cttgtaatat cgcacacttg gatgctattt gttgtagtaa acctgcatac cgtggactgc
    13621 catcacgagc aacattatac aaacactgac taaaagcatc gaacaaacaa tagatgtgat
    13681 cactagtgaa aaaaggtctt aatttttttt gttcttcgtc gctgtaatcc aggagcatct
    13741 cattgatttc ttcctgtgag aattttaaga caaaattatc ttcaggattt gtttctttgt
    13801 tgtttttttc aatattcttc tcctcaatgc tattcgcatt ttgtttcgca attatttccg
    13861 aatttttttt cgcagttttt tccgcagttt tttccgcagt ttttttcgca atttcttctg
    13921 caattttttc ccgagttttt gattttagac taacaccatc gatgtcaacc ggtgttgttt
    13981 tcagatctct ctgcgcgtct ttcaaatttg tcttatagag atcgggcact ttatcggcag
    14041 ataggtatcc ggatgaagaa atctggtgtg tacacatggg gggctgttca caaagcatcg
    14101 aggttatatc gtctttaaac tcttttacat taaacttctc ggcattaacc ttccctgtta
    14161 taaaccccgc tttggcaatt ttcttctcta attgttttgt acgtttagaa taataagcgt
    14221 tgttatcata aaacttttca agaagggccc actcctcaag ttgccttatg tgatttttca
    14281 actgttgatg atctatttta tattcatgtg tactagaatc attttggttg caggagttac
    14341 agatttttaa gcataatttt ccatcagatt tatcatctat ggtatcagtg aaaatatagg
    14401 acacttcaat attctgcccg ttcttatcag tcatctcata tgaccattgc cccgttcgtt
    14461 tcgcattctt aatgagttcg atggactctg caggacaacc atgaagaatg ggcagaattt
    14521 tatcaggata cttaatgagt aaaattatta tatttacaac gtttgaaaag aaatttttgc
    14581 tattttttat ttcatcgatt ttttccgctg aaatgtttac taagttttca tttccgcaaa
    14641 tgaaaagcga tttgccatgg gtgctttttt taaattgttc ttctgtttta gaaaagacat
    14701 cctccttaaa aggtggcaac tcagcaccag taataaacgg tttcaataaa ttcgaatttg
    14761 aaattgtcat atctttgctc cactacaggg ttatcctgga gcatgttgta cataacatca
    14821 tgggcaaaga ataccaaaaa tcgctcatat cgatcgggac aaatatttaa caaattcagc
    14881 cctgacattg agccgctttt ttgcgctgcg tagtgtcagg gctggtgact aacgacgtaa
    14941 caaataaaac agatgcttta gttcatccac accgtagccg aaatggcagg caaagccaga
    15001 atcccgtctg tcagttgccc atggccttct ttgcgttgcc attgcgcgac attaagaaac
    15061 ggtgacgcgg ggagcaccac ttcacaggcc tcgccacggt tgattgccac cagtacacgc
    15121 tgctgattta gcacgcggac aaataccacc acgttatctt ccgcatacag tacctgacag
    15181 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgctg gtacagcgcg
    15241 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    15301 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    15361 aacagccaga ccaccgccag gggcaggcgc gcaacatccc gaccgagcag cgttttaaat
    15421 cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgctgttg atgagaaagc
    15481 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatcg
    15541 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc gcgatagttc
    15601 atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgctctcca
    15661 actatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccagc aacgtgctgc
    15721 aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag ccgccagccg
    15781 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    15841 tcattcacca gactttccga ctgataatcc agcttcggca agctggcata gccaagccag
    15901 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccaaggcga ttcggggttg
    15961 tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg
    16021 tggttaaaca cgccgtccag caccagacgc attcccagct gctgcgtatt gtgtcgcaaa
    16081 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    16141 tatttatgta cgctgggagc tttaaacacc ggattgagat acaacgccgt cacgccaagt
    16201 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    16261 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca atatgatctc ttgtccggct
    16321 gcgtgatggt aatagacatg atcctgttca gcttcacgag gaagactacg ggcgaaacga
    16381 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgaaccgat atccggtaca
    16441 tcgatggcaa actgctccag tcgtgctggc ggcattcggc tgaagccttg cggtgtaaac
    16501 caacgctggc gatcgtgcca aagcaatttg aaactataac gccgccgggg ttgtccgctg
    16561 gagagatcga tcgccgcccg ccatgccgtg acgcctggct gcggctgact gcgctgctta
    16621 tgcatcggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    16681 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    16741 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    16801 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    16861 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    16921 aacgcaagta ttactggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    16981 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    17041 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    17101 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    17161 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    17221 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    17281 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    17341 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    17401 cgtcactgtt aattgccgac agcgaagcag tcagcacaac aaagttaaga atgctggcgg
    17461 caaaggtaat gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct
    17521 gattccacgg gtagatagac ataatgacga acagtgtacc aacatagaac accagaatac
    17581 gcatcggcac ggagttaatc gcacgcggaa tagatttctc aggatctttc gcttcaccgg
    17641 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    17701 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    17761 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    17821 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aataccttca
    17881 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    17941 gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    18001 caataaggat ttcaaagcag taggtccaac cggtaatgta acctgccagc gggccgaggt
    18061 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    18121 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    18181 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    18241 ccagtgccat aaagcgtatg tgtcgggtac ttagcccacg ctttagcttg ttcttacttt
    18301 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    18361 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    18421 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    18481 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    18541 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    18601 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    18661 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca
    18721 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    18781 caaagaattc tgcacaagca caggtcaggc caactgccgt taccaggcag gcgatgaaga
    18841 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    18901 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga
    18961 acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat
    19021 aacgggtcag cagacgcgct tcagtaacgc cacgagaacg cgccgcgtta acaataacga
    19081 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    19141 aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    19201 tcgcggcaac agacaggatg accagcgcga taattttcag cggtgcaagg aagttgccca
    19261 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    19321 ggctgtagat aaacagcggc agtgcggaat cacccgtcag cggcgcaatc cccacttcaa
    19381 aagaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacacaccg
    19441 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    19501 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    19561 cgaatgccgc agtccagacg tgttcgcctg cctgcaagcc gaccattgga gggaaaataa
    19621 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    19681 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    19741 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    19801 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    19861 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    19921 accagaggta tatagcgata agaaatcacc aactagtata aaacactatt tccgtgaaaa
    19981 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    20041 tttgactgca aagaaaataa gacaacatcg gctggcttat ggcaataaaa gatgacaaag
    20101 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaagct gaaacgtgtt
    20161 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    20221 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    20281 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    20341 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    20401 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    20461 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    20521 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    20581 agcaaatgcg tcggtgcggc ttctattttc gacagcgtca gcaattgctt caccaaccct
    20641 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    20701 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtgcgtaac
    20761 tcatggctta cgttggcgaa aaagttgcgc cgcgcccctt ccagttgatg catttgcgtg
    20821 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    20881 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    20941 tgcgtaaact ccgggtaacg cagcaggtta aggatgttct gcccgttatc ttccggccag
    21001 cgcaaaccaa gaatctgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    21061 gtcagcacca ctgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    21121 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    21181 ggttcccagc taccacgccc cggtggcggg gtcatactac gatccaccca cagccaccat
    21241 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ccgttaccga tgccagcaaa
    21301 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    21361 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    21421 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    21481 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    21541 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    21601 acgtggttta acagctgctc gcggctgtac acgcgttcag gatgcgtcat aaaaaagtgc
    21661 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cacccggtga
    21721 gatgtcgggt cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa
    21781 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg
    21841 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    21901 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    21961 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    22021 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    22081 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    22141 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    22201 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atctcttcag gttattgatt
    22261 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt
    22321 ttcgccaggg aaccgttatg cgcattcttc acacctcaga ctggcatctc ggccagaact
    22381 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggaaacag
    22441 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    22501 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    22561 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    22621 atatcatggc gttcctcaat acgaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    22681 tgcctcgtcg cgacgggacg ccaggcgccg tgctgtgccc cattccgttt ttacgtccac
    22741 gtgacattat taccagccag gcggggctta acggtattga aaaacagcag catttactgg
    22801 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    22861 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg
    22921 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    22981 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    23041 ttcgctattg cggctccccc attccactga gttttgatga atgcggaaag agtaaatatg
    23101 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    23161 taacgcaacc catggcggtt ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    23221 agtggcgcga tgtatcacag gagccgcctg tctggctgga tatcgaaatc actactgatg
    23281 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgttgaag
    23341 tattgctggt acgccggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    23401 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac
    23461 tggaggaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg
    23521 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    23581 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    23641 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    23701 tcacgaaact ccacgcctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    23761 taccgccgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    23821 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc cacgcgtaga
    23881 gctggcgcgc tgcgccgatg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    23941 gacggcgacg ttaaccggac tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    24001 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    24061 gttaactggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    24121 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt tgctcacgcc
    24181 agaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    24241 acttaccgcg cagcagcaag aacaacaatc actaaactgg ttaacgcgtc tggacgaatt
    24301 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    24361 agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc ttcgtccgca
    24421 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat actcgccagc agattgaaga
    24481 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    24541 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    24601 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    24661 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    24721 acttaatacg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct
    24781 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    24841 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    24901 acagacgcaa cgtaatgccg cacttaacga aatgcgccag cgttataaag aaaagacgca
    24961 gcaacttgcc gatgtcaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    25021 tcaacgtgca cagttacagg cgggtcagcc ttgcccactt tgtggttcca ccagccaccc
    25081 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    25141 ggaaaacgaa gttaaaaagc tcggtgaaga aggtgcgacg ctacgtgggc aactggacgc
    25201 cttaacaaag cagcttcagc gtgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    25261 agcacttact caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca
    25321 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    25381 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    25441 tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg gttatgcact
    25501 gacagtgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    25561 gagctggcag caacaccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    25621 gccgattctg gaaacgttgc cgcaaagtga tgaactcccg cactgcgaag aaactgtggt
    25681 attggaaaac tggcggcagg tacatgaaca atgtctcgca ttacacagcc agcagcagac
    25741 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga
    25801 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctagtgga
    25861 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    25921 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcagc aacaccgacc
    25981 tggcgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    26041 ccaaaaattg cgtgagaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    26101 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    26161 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    26221 caaatttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    26281 ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    26341 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    26401 tttcctcgtc agtctggcgc tggcactggc gctttcagat ctggtcagcc ataaaacacg
    26461 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    26521 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    26581 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaatggact
    26641 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    26701 tacagagggg cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    26761 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    26821 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg
    26881 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    26941 tattatgcgc tgggggtggt ggtcggtgcg ccaataatcg cactcttttc cagccgctac
    27001 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    27061 acgctctctt cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat
    27121 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    27181 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    27241 ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt
    27301 tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg cgacgaggcg
    27361 aaaggaaagc tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc
    27421 gccgccacca tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt aaagccatac
    27481 atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat gttagttggg
    27541 ctagggatgg tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg
    27601 cgcattgcag cagtgactga ctttataatt gtactggcac tgctgatgct ctttttctgc
    27661 ggcggtatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc
    27721 ctttcagcac ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt
    27781 gccgcaggtg ggcaaatagc ctttaacctc ggtagcgccg tcggcgcata ttgcggtggt
    27841 atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg
    27901 tttgctgcga tgtcgtcatt gctgctgtat ggtcgctata agcgccagca agcggcggat
    27961 actccggtgc tggcgaaacc tctggggtag attgcagtca cggagcacgt tgcttattgc
    28021 cggatgcggc gtgaacgcct tacgcggttc tggcacattt tgtaggcctg ataagacgcg
    28081 gcaagcgtcg catcaggcat gatgcgccaa ttgcctgggt tttttactct tgtggccata
    28141 accacgcagc gccgcgcacg ccgctggaat caccatgctt cgccttacgc accggcgttt
    28201 cacattcgcc gccgaagaca aattgtttaa tcaactgcgg aaccgtttga tataaacggt
    28261 ctacattgct catcccgccc cccaggacaa tcacatccgg atcgagaata ttcacgacat
    28321 gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa tgccagttcc gctaccggat
    28381 cgctttcttc aaccaggcgg ataatttcac tgcctttcag cgcatgtccg ctcaaacgac
    28441 gataatccgt cgcgaatccc gtgcccgaaa taaaggtttc aatacaacct tgtttaccgc
    28501 aataacaagg gacttcctcg cgataacgca gttcgtcttc gtccatccac ggtagcggat
    28561 tgtgtcccca ctcacctgcc gtgccattgc cgccgatatg cgcccgccca ttgaatgcca
    28621 cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac cgtctgcgct cccgctgccg
    28681 cgccatctac tgcttctgaa accgccagac agttggcgtc atttgccagc cgcacttccc
    28741 gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag gttgaattgg
    28801 cattcttcac cacaccggtg taaggcgaaa ttgagccagg aatgcccata cctaccgttc
    28861 cgcgctgccc cgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt tcaatagtct
    28921 gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc cctgcatcgc
    28981 ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc acggtactct
    29041 ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca atgcaaggcc
    29101 gccgacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa attatcatgc
    29161 tgtggttcaa gaatttaatg gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag
    29221 agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag gacatggcga
    29281 agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac gttgccaatg
    29341 gtcaaattgt tatctgcgca cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac
    29401 aggcgctgga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa
    29461 ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gttgccgcgt gctttcagcc
    29521 gttttagcca gacaatgatg tggatcgaca cggttaacgg tttgattatg gtggactgcg
    29581 ccagtgccaa aaaagcggaa gatacgctgg cactgctgcg taaaagcctg gggtcgttac
    29641 cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa tgggtacgtt
    29701 ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc cgagctgaaa tcgttgctgg
    29761 aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag atcaccaatc
    29821 acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag cgcattcagt
    29881 ttgtgatgtg cgacgatggt tcgctcaagc gtctgaagtt ctgcgacgag ctgcgcgatc
    29941 aaaacgaaga tatcgaccgt gaagatttcg cccagcgttt tgatgccgat ttcatcctga
    30001 tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt ggcgaagcac
    30061 aacgttaatt gctgatttac ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc
    30121 gtacgaagcc acaccaggca tataattatt cgctacggcg ggcaataatt tttagcgcag
    30181 caatattatg cgttttacgc cgtaactgat ctaatcgcct catccctatt cgcctgtcag
    30241 cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt gttaacagct
    30301 ccgcttttgg ggtgtcaata tattcacgca ataaatcaga aattgaacaa atatccgtca
    30361 ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccaccgtta cctgcaataa
    30421 agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa cgcggtaaat
    30481 cggtgcttcc accattttgg ctgcgcatgg gataacgggt acagtgaatt tgcaggttga
    30541 tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccatggg cgctgtgtca
    30601 ttgtttttcc tcaagccggt gacgcgcttc actgcgaatt ttccgcgttg ccccggtttt
    30661 cactaaatca cgtaacagcg aaagatcggg ttgttcgaca aataagagta atgatggatc
    30721 ctcttttaac cacgccgttt tcacatcggc agccagctgt ggattattaa tagccagcga
    30781 ccgatcctga ggctcagtca atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa
    30841 tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc attcatcatc
    30901 tgatttatct tttatcgtcg ccagttccgc aaaaatatcg ctatcctcat ggttccagcg
    30961 gtgtaatcct ctttcacttt gtctacgggt gctatttttc atactgacaa cagccagcgc
    31021 ccagtcagag ttgaagcgat actgttgatc gagtttgttg ataattccgg aaggtaattc
    31081 tttattatca aataattggt tacgcattcg atagcggtat tcagcgggta aatcaaccag
    31141 taaatcaatg gcattattta acatctcttc gtcaacaaca ccattatcaa tcagcgccag
    31201 ctgtacttcg taggcttgct cttgatcttc ttgccgtttg ctcatcatta aatttaatgt
    31261 gcgttgccat aactgtttct tatattccag cggcaataat ttatcgcgtg ccagctcatc
    31321 ccagacggtg ggaacatcat gctgagtcag tagataacta atcacttcct tcgatgtcag
    31381 ataacgcgta ccttcgcgta aattgtgcac atcttctttt gccagagaca gttgacgcat
    31441 ctccggcaac gctatcaata acgcttttac gatattttta ttttctttat tatccagata
    31501 catttgttct gcgatacgtt caatatcctc tgtacgcagg gttgctgaca gttgggtaat
    31561 ttttaattca gtaagcgtcc gggctaatgc ttcacgaact ttcagtgatt tatctttact
    31621 caacagcacg gcttgttgtg cggttaaatc tgctccggta attctttcgc gtaactccgg
    31681 atcttcgcta tttatcaact catcggccac tgctgtcgga accagccagg cgtaatcgta
    31741 atggctgagt aatgtcttgc gtacattgag aaaggggcta tgacttaacg acgcaacaat
    31801 atctggagaa agattatcct gtctggcctg ctccagctgt acgctctcga cgggtacaga
    31861 cattaatttt ttattcacat cggcggaaat attactgttt ttaaccagcc ctctggcaac
    31921 agcctcatct ttaattaatt catcggtaag caaatgcatc tgttgagcgg gccaggactg
    31981 cgccatcgat tcacgcgtct ggtcatcggg gtgctgtaac agttgcagca taacggcggg
    32041 tgataaactc tctgtatgag tcagaaaacg tgccaccagt ggatcttgat gctttatttt
    32101 attcgctggg aaattatcga gtttccccgt ttcaatccat tttaatttct cattaatttc
    32161 aggagcggct ttatctcgaa aaagcggtaa accctgaacg atgttgtctg cgacgctatc
    32221 atagtcagga tacgcattgg aaatgttatc ggtttcgatt tcagtgtgat cataaagatc
    32281 ggcagtcacc agtgaatagg ctttttgaat attctcatcc cagttatatc cttcaccact
    32341 tccatcttca ttgatataaa attcagcttt aatgacttca aattgcgttg ctttatcgga
    32401 aacaataatg cctcgcgcac taattccctt catcacttca aagttatgat cattgtaatc
    32461 agcataaact aaacctttcg ctgtcatctg tcctttgaca tacgcgaaat catgattaat
    32521 aaagttatcg acaataacat tacctaatac aatcagatga ccattacctg acagataatc
    32581 atcataatcg ccggaaatcg ttaaattacc gttcaccacc agtttatttt gattgctgaa
    32641 gttagcgggt aatgataggt taccattatg ataataccag cggatattat cttttagcat
    32701 ataagcagct tccatactat cgtcggttgc cgctttaacc acgttgcgct catctgccgt
    32761 cataagatga aagtcagagg tgataaggtc ctcgctactg atccactctg tggcacatgc
    32821 atagccagtc ataaaaaata agaccataca gatgaccaat atattaattc tattcataat
    32881 cttcaatgat atttatatca accttgatga attatgaata tatcggaaat tgaaaaaatc
    32941 gcagcaaagg aaatattatt atgggttaaa gagaggatat ttcagaaaaa tgccctcccg
    33001 ttcaagggga aggcgaggaa ttacagatag cggcacagat aagaggtggg ttcggcaact
    33061 tgcagatgaa actcactgtg accgggaaca ttaaacaccg aaccggcttc atacacttgc
    33121 cagtcggtcg cgtcaggcag taacacattc agcgcgccac tgattaccgt catctcttcc
    33181 ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa cacccacgct ggcgcgacca
    33241 gtgctgctgc tggaaaagcc gatcgatttc actttgccgg aaaagtactc attactttga
    33301 agcataaact ggccctgtct ggtagttgaa catctacttc actatagggg ccagaggcgc
    33361 tggctgtcac gcaaaattac accagtaatt ccgcagccaa ccgtgcaatc aatacgttag
    33421 agagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgc tggttaaatc
    33481 ccaaacaatc cagcatgatg acatctgcgc ctttcgccag taattctttc ccggcatcaa
    33541 tgattttttg ttctgaatca tgaatggggt tacccaatga aaataccggc ggtttctgca
    33601 aaatttgcca tttttgcgcc tgaacggtca gcaactcctc aaccggtacg ataaccccca
    33661 cctgatgatc ttcaacaata gaggaaacca gcggtggcaa tatccgcgac ggctcaagaa
    33721 agatcgtatt acgcgcagtc atactactaa tgtttgccgt actcattaat ataatgacgt
    33781 cgtagccctg attatcgagc acttcaacca caccttgcag gtcacgctct actttgcgac
    33841 gcgaaacatg ggccagatgg ttgtcattta ataatgtgag aattgtgtct tcgcctgctt
    33901 ctggcgcgta ctccgccatc acttcttcgc ggcttaactt ccccagcagg ctatgatggg
    33961 aaatattatc ttcgtcaatg tattctgtca ggagcggcaa aacttcctgc ataggtacaa
    34021 tgccgatggt tagaatcgcc aacgacgcac tcattgttct tccaccttac ttttatttcg
    34081 tcgttaatta ccgggcaagt gttaagcacc actgctgaca cttacttgtg ctacaaatga
    34141 caaaaagcgt agcagcagac gtgcacggca taatgtaaag attccaaatg attccagtaa
    34201 tggattgtta ttgtttaata ttctaattat ttgaaaaaca tgaattatga aaaatgtgac
    34261 gcagatccct tgtagtggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt
    34321 gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagcggtag gatgttcact
    34381 gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc
    34441 tggttttcct ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg
    34501 catcgttctt ccttatttca cgggatgaac gttaagtata ggcgctctaa aatcaacaat
    34561 tgatcgtctg tgccagggcg ctacgaattt cagaaatcac ctggctgggt tcgtttgttg
    34621 cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct
    34681 gaacttcttc gctcagaggt tttcccgtta aggttggccg taaatcttct tccggtgcag
    34741 cttgcagtcg gttaaccagg actgaaactg gcgcacacaa ataaaccacg atcccgttat
    34801 tttgcatgaa gtgacgatta aattccgtca gaataatgcc gccgcctgta gcgataacgg
    34861 tggatggcgc agttaccgct tccagcgccg ccgtttctct ggcgcgaaat cccgcccact
    34921 cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac cactgatcgg
    34981 tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac
    35041 agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg
    35101 caatgcggcg aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt
    35161 cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca atacatccct tactatacca
    35221 ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac tacgttgcac
    35281 ttccagccac cacttctcca gctccgcggc aaatgcctgg cggtcacgtt gtgaaaggct
    35341 atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt
    35401 caggcgctca cgaatggtcg ctggcgtgta acgttcaccg cgcggattaa gcgccgcagc
    35461 gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc
    35521 ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt
    35581 acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat
    35641 ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac acgcgtcggc
    35701 atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt
    35761 ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc
    35821 acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa
    35881 aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat
    35941 ataccccctc cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag
    36001 aatcggcgca agaagtggcg caaatcgccg acatcatttt tgctgccgtt aaacctggca
    36061 tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt
    36121 ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gatcggaaaa
    36181 ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc tcggtaacgc
    36241 caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg
    36301 gcgaagcgga agtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt
    36361 cgccagccta cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga
    36421 tgccacgctc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg
    36481 tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca
    36541 ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgctgca gtgatcgaag
    36601 cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg
    36661 acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt agagcgccaa
    36721 atctgccgat ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc
    36781 cacactaatc cgtaaagtta cctgtggcgt attcggcaaa cgtaatgtat ttagcccttc
    36841 atgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc
    36901 aaactcatcg ccgccaaacc gaccaatcac atcgctacca cgcagggtaa tttgtaactg
    36961 tcgggtaagc gccacaatcg cttcatcgcc cacatcatgg ccccaggtat cgttgatgct
    37021 cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt
    37081 atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc
    37141 gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttag tcgccgtctg
    37201 gtagctgacc cagccaaaca gcagaggata aatgacaata atgggaaggg agagccacca
    37261 ttccagcggc gcactattga acgacacggt gatgcccgtc agctcgaggg tgacaaggca
    37321 ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa
    37381 attcagacac ataatcatca acatcgcggt ggaaggcagc acgtttacgc ccattacgcc
    37441 tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg
    37501 atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac
    37561 caacaccagc caccaccagc ccggcggcgg gtgtgaaacc agcgttgaag caatcggtaa
    37621 gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga agcgcagccc
    37681 ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccgtgcg ccgccgcttt
    37741 tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat tttcccaaat
    37801 tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccattt tagtagctca
    37861 cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcgctcat aaacttatta
    37921 cgatcatcac ctttcagtga ttgttgcgtt gcttgattat tacattcgcg catcttttgc
    37981 tgctgtggtg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag gcaatcactc
    38041 atgtaggtct tacgagcatc ccctttcaac gcctgtgccg tcgcctgctg attacaggag
    38101 gtcatacgct gttgttgtgg ggttaaggtt ctctcggcag cgccgacggt ggttaaaaaa
    38161 accagaccga aaagcaaggt aaccagtaat gtaattttca tagcaccatc cctcttcatg
    38221 ttttaaccat gagcgtatgc gcccgtgatc tgccattaag tctggttgct aacagcaaaa
    38281 aaaccacccg gcagcgaaaa ttcactgccg ggagcggttt tatttcagcc ccagagcggc
    38341 tttcatggtg tagaagagat cggtctggtc ggtcagtcca acgacattgg cggcatgcgg
    38401 gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct cttcggagtt
    38461 cccgtaactc atcaccatca ctgcgccatc tttggtattt agcgcctggg tgaggcccgg
    38521 agctttggta tccggcgcaa caatctggct ggcgtgggcg tgatcagcgg tgactatgac
    38581 cagcgtgtta ccatcctttt tggcgaattc cagcgcccgt tgtacggctt catcgagatc
    38641 gaccgtctcg ccaatttgcc cacaaggatt cgcagcatga tcctgtttat cgattgacgc
    38701 accttcaact tgcaggaaaa agcctttctc atttttactc aataattcaa tggctttgtc
    38761 ggtcatctgt gccagggtcg gtacactgtc attacgttgc ggattaggcg tacaggtgac
    38821 tgcgggctta tcgatattac cgtggtacgt tgctttcggt cctagccagc gcactggcat
    38881 attgccgtca gcaaacaggc caagcagggg tttttgctga ttcgcttccg tcaccgaatt
    38941 cagtgaggca gcatcgctca ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt
    39001 ttttccctgc cattcaccag cggttgccgt ttcagcaaag gtttttgcgc cgccgccaag
    39061 cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc gatccttttc cgcctttttc
    39121 cagagcgtta cccggacatt tttcactggt cgcgctcgga ccgtagcatt tgcgcgaagt
    39181 cacatgtgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag aaacgttacc
    39241 ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat
    39301 atcgacgccc agcgcgccgt tataggtttt gacaccggtt gaccaggcgg ttgctgatgc
    39361 agccgagtcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat agtgagtgta
    39421 ttgcccggta agcggtaagg catcgatacc attaaaaaag ccgcccgcac cttcggcata
    39481 attacgtgcg gcagtaattt ccgaatcccc catcccatcg ccaatcagca aaataatatt
    39541 ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcag cagttaagcg
    39601 gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat
    39661 ttctggtgtc cgggcttttg tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat
    39721 agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata aaaacaaagc
    39781 gactataagt ctcggccgtg acaactttat gacatctgtt gaaaagatta actttttact
    39841 gacgaggatg cttcaataac ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag
    39901 gaatgctggc atcgggcttt acctcgtaca gcgccccctc tacctgatca atcaaccgtt
    39961 gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac gataatctcc aaagcttcta
    40021 cctgcgcaca cagttctttc gactcttctt ctttttgggc aagcttaaat aacaactcag
    40081 caatgagatt tttcatgtct gtatttcctt atccaaagta tggagaagtt aacattatgt
    40141 ttcacaactt cat
//
LOCUS       DAZGCQ010000040        39783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000040 DAZGCQ010000000
VERSION     DAZGCQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.040"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..290)
                     /locus_tag="ACHK07_002209"
     CDS             complement(<1..290)
                     /locus_tag="ACHK07_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8102536.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDF"
     gene            complement(482..760)
                     /locus_tag="ACHK07_002210"
     CDS             complement(482..760)
                     /locus_tag="ACHK07_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102537.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(1140..1328)
                     /locus_tag="ACHK07_002211"
     CDS             complement(1140..1328)
                     /locus_tag="ACHK07_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO8102538.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(1706..2083)
                     /locus_tag="ACHK07_002212"
     CDS             complement(1706..2083)
                     /locus_tag="ACHK07_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8102539.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(2088..4784)
                     /locus_tag="ACHK07_002213"
     CDS             complement(2088..4784)
                     /locus_tag="ACHK07_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO8102540.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLVGRE"
     gene            complement(4922..5314)
                     /locus_tag="ACHK07_002214"
     CDS             complement(4922..5314)
                     /locus_tag="ACHK07_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO8102541.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(5350..6837)
                     /locus_tag="ACHK07_002215"
     CDS             complement(5350..6837)
                     /locus_tag="ACHK07_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102542.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEHQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(6827..7735)
                     /locus_tag="ACHK07_002216"
     CDS             complement(6827..7735)
                     /locus_tag="ACHK07_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102543.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTVASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNAHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(7735..8397)
                     /locus_tag="ACHK07_002217"
     CDS             complement(7735..8397)
                     /locus_tag="ACHK07_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102544.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(8394..8756)
                     /locus_tag="ACHK07_002218"
     CDS             complement(8394..8756)
                     /locus_tag="ACHK07_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO8102545.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(8769..9083)
                     /locus_tag="ACHK07_002219"
     CDS             complement(8769..9083)
                     /locus_tag="ACHK07_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO8102546.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(9160..9402)
                     /locus_tag="ACHK07_002220"
     CDS             complement(9160..9402)
                     /locus_tag="ACHK07_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102547.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(9402..9743)
                     /locus_tag="ACHK07_002221"
     CDS             complement(9402..9743)
                     /locus_tag="ACHK07_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO8102548.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            9856..10422
                     /locus_tag="ACHK07_002222"
     CDS             9856..10422
                     /locus_tag="ACHK07_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102549.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(10806..11567)
                     /locus_tag="ACHK07_002223"
     CDS             complement(10806..11567)
                     /locus_tag="ACHK07_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO8102550.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSAPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(11560..13656)
                     /gene="traD"
                     /locus_tag="ACHK07_002224"
     CDS             complement(11560..13656)
                     /gene="traD"
                     /locus_tag="ACHK07_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8102551.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(13653..14441)
                     /locus_tag="ACHK07_002225"
     CDS             complement(13653..14441)
                     /locus_tag="ACHK07_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102552.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQVFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEVLSALPEKALPRSWWRRLLQRGTRS"
     gene            complement(14438..14920)
                     /locus_tag="ACHK07_002226"
     CDS             complement(14438..14920)
                     /locus_tag="ACHK07_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102553.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLNGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MLAVVLFLGALFMEGVSSVILFRGCGSAAVSGEGRGSPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(14917..15510)
                     /locus_tag="ACHK07_002227"
     CDS             complement(14917..15510)
                     /locus_tag="ACHK07_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO8102554.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYRRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGES"
     gene            complement(15507..16025)
                     /locus_tag="ACHK07_002228"
     CDS             complement(15507..16025)
                     /locus_tag="ACHK07_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO8102555.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(16022..16702)
                     /locus_tag="ACHK07_002229"
     CDS             complement(16022..16702)
                     /locus_tag="ACHK07_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8102556.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(16681..17400)
                     /locus_tag="ACHK07_002230"
     CDS             complement(16681..17400)
                     /locus_tag="ACHK07_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102557.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(17404..18153)
                     /locus_tag="ACHK07_002231"
     CDS             complement(17404..18153)
                     /locus_tag="ACHK07_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102558.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESARKKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(18150..18749)
                     /locus_tag="ACHK07_002232"
     CDS             complement(18150..18749)
                     /locus_tag="ACHK07_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO8102559.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(18935..19927)
                     /locus_tag="ACHK07_002233"
     CDS             complement(18935..19927)
                     /locus_tag="ACHK07_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8102560.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(19976..20536)
                     /locus_tag="ACHK07_002234"
     CDS             complement(19976..20536)
                     /locus_tag="ACHK07_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8102561.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(20552..20755)
                     /locus_tag="ACHK07_002235"
     CDS             complement(20552..20755)
                     /locus_tag="ACHK07_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102562.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(20846..21313)
                     /locus_tag="ACHK07_002236"
     CDS             complement(20846..21313)
                     /locus_tag="ACHK07_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO8102563.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
     gene            complement(21992..24034)
                     /locus_tag="ACHK07_002237"
     CDS             complement(21992..24034)
                     /locus_tag="ACHK07_002237"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8102564.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGCLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(24037..24741)
                     /locus_tag="ACHK07_002238"
     CDS             complement(24037..24741)
                     /locus_tag="ACHK07_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO8102565.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            24787..25644
                     /locus_tag="ACHK07_002239"
     CDS             24787..25644
                     /locus_tag="ACHK07_002239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102566.1"
                     /translation="MRITDNKKTNQAEYTFNADYFLIAVDTGNTAHFLTSVATGSTVR
                     FLTSAATGSTVHFLTSAATGSTVRFLTSAATGSTVRFLTSAATGSTAHFLISAATGST
                     VHFLTSSATGSTVRFLTSAATGSTVHFLTSTATGSTAHFLIPAATGSAAHFLISAATG
                     STAHFLISAATGSTAHFLISAATGNTAHFLITVTTGSTVRFLTSSATGSAAHFLISAA
                     TGSTAHFLISAATGNTAHFLITVTTGSTAHFLTSVATGNTAHFLITVATGNMVNDDYY
                     LNVQRQKQH"
     gene            complement(25570..26049)
                     /locus_tag="ACHK07_002240"
     CDS             complement(25570..26049)
                     /locus_tag="ACHK07_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102567.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(26161..27405)
                     /locus_tag="ACHK07_002241"
     CDS             complement(26161..27405)
                     /locus_tag="ACHK07_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO8102568.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEGRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(27423..27890)
                     /locus_tag="ACHK07_002242"
     CDS             complement(27423..27890)
                     /locus_tag="ACHK07_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102569.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(27894..28313)
                     /locus_tag="ACHK07_002243"
     CDS             complement(27894..28313)
                     /locus_tag="ACHK07_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO8102570.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            complement(28328..28936)
                     /locus_tag="ACHK07_002244"
     CDS             complement(28328..28936)
                     /locus_tag="ACHK07_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO8102571.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(28933..29658)
                     /locus_tag="ACHK07_002245"
     CDS             complement(28933..29658)
                     /locus_tag="ACHK07_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO8102572.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(29651..31384)
                     /locus_tag="ACHK07_002246"
     CDS             complement(29651..31384)
                     /locus_tag="ACHK07_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO8102573.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHYSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVFIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGMGDCGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMAGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(31384..32751)
                     /gene="dnaB-PI"
                     /locus_tag="ACHK07_002247"
     CDS             complement(31384..32751)
                     /gene="dnaB-PI"
                     /locus_tag="ACHK07_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFO8102574.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAPH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRASARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENGG
                     PGGYA"
     gene            complement(33099..34001)
                     /locus_tag="ACHK07_002248"
     CDS             complement(33099..34001)
                     /locus_tag="ACHK07_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102575.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            complement(34237..34446)
                     /locus_tag="ACHK07_002249"
     CDS             complement(34237..34446)
                     /locus_tag="ACHK07_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102576.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(34779..35666)
                     /locus_tag="ACHK07_002250"
     CDS             complement(34779..35666)
                     /locus_tag="ACHK07_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8102577.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     REFFDALLENGGKSLWAEN"
     gene            35982..37166
                     /locus_tag="ACHK07_002251"
     CDS             35982..37166
                     /locus_tag="ACHK07_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8102578.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            37495..38076
                     /locus_tag="ACHK07_002252"
                     /pseudo
     CDS             37495..38076
                     /locus_tag="ACHK07_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            38163..38441
                     /locus_tag="ACHK07_002253"
                     /pseudo
     CDS             38163..38441
                     /locus_tag="ACHK07_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(38709..39713)
                     /locus_tag="ACHK07_002254"
     CDS             complement(38709..39713)
                     /locus_tag="ACHK07_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8102579.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTINQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
BASE COUNT         9330 a        11997 c         9214 g         9242 t
ORIGIN      
        1 tggaaatccg gatgttcctc aaacagcgcc agactggaaa aagccaccgc atccctgaag
       61 tacaccgacg atggttcatc tgtaataagt tgtctgatca gacattccgc cagcacggac
      121 atctgtggcc ggatctgtaa aagcaaagac gacgggctga ctgccatacc ggataatggc
      181 gtgcagaacc gcagataccg cctcaccacc tcctcactga ggtcagcgat aaggaaacgt
      241 ccggtatgag cctgaaaagc agggcgcaga tgcccggcaa tcagaagcat tgtgccggca
      301 ggtatgacag agttcctgtg ctccagacaa agcattgtct gtttgagagc cataatgaca
      361 acgcaggtac agggcatcgg atatccggta aatatgttga tttattaaag agacagacat
      421 gccccgtata taaaattccg tgtcaccggg tgctgaaaaa aactgcaccg acggcgctct
      481 tttactcctc cgttacggat atttcagccc ccttttcagg agcggtatcc ttcacctcgc
      541 tgagcccgga ctgagccgct tcagaaacct ctgccacctt tccggcagcc tgcattttca
      601 cttcctccgc ggcatccttc acatcactga gttgagtctg cgcagcttcc tttatatctg
      661 ccgccttctt acttacattc tcctttgtcg catcatcaca gcccgcaacc agaaatggtg
      721 cggaaataca taacgccaga attgtttttt cagcatacaa tctctcatta ttaagtgtta
      781 acgcagaaca gcttatattt cgtccccggg aaatggtcaa aagacaacca gaacatcaca
      841 gtgtcagggc acactcccgg tatatgcttt tataaccata acatgctgtt cctgatatta
      901 ccggaaatgt gcccactaca tttaacctgt tttcagcacc gggtcatcgg ctgtgctgta
      961 ttttttattc ccgtcacaga aacacactat taccgttgat ttcatgacga ataaaatgaa
     1021 taaattctcc cgtggtcttt accaccatta ttataccaat aacaccattt acacagatac
     1081 actccgggac tatctgtttt tgccccctcc gactccgccg gaggttttta ttttcatact
     1141 caggccttca ccacttcaca ccggaagtcc tccaccctga cgccaatata ccgtcccggc
     1201 tcttttgcgg gcgcaaacag tttttcacat tcatcctgag tcattttttc atcctggtaa
     1261 cgcagccact gaaccggcat attgcctggt ttgattattg tcgcagttat tttatatcgc
     1321 tttcccatac tggctctcat ctcaacaaca aaatcaccgg ctgagccatc actccggtaa
     1381 tgcatgcgaa aaaaagtgag cgtctcagca atggctcatg cggtgattct gtacggtacc
     1441 cgcacaaaat acgggcacca acagcgtcgc agaatacaac gaaggcatca gggcagcacc
     1501 tctctcccgc tatttttcag agttctttac cctcctcctg acacgggagt acaaccctga
     1561 cagcatccgg gccagtacat tcgcaagccc ccatgaaaac acagccacac ccacagctcc
     1621 cgccagaaac agcacaggtt cactggcaat ttgatctgac agcgatggat acagtggtcc
     1681 cggaaaatcg atatcgctcc tcatatcacc cctctcccag ccacaacagc agatagaaca
     1741 aatccgccgc atcagtcact gatatatacc agcggggatg aatatactcc tcctccgacg
     1801 gccagctctc atgcccactc acagtaagca gaatatgcac atactgcccc tgctgacctt
     1861 ccagcatcac cgccacactg ctgttcagac ccaccctctg tggctggcaa cggatacact
     1921 ccacccgccc cagttgcgcc gccagatgcc cgcgaagacg ttcaagatta tcccgtgtgg
     1981 tgcaacttat ccccggcgca ccctgtggcg gcagaacaaa cacacttccg gcagggtgaa
     2041 cagcagacac cacttccggt tccggatact tcagacacag catagtctca ctccctgcca
     2101 accagaccac gtagcccgtc cagctttcgt gccacatgaa acgctgcttc cagttcgctg
     2161 cactggtgca ccttcatcag ggcgcgtcgt tcagccttct cttctttctc cgtcatcccc
     2221 agagccagat acagactcgg tggcacaacc cggaacaacg cttctgtgcg cttcgccagt
     2281 accaccccct ccgtatagca gccggacagt ttactggctg acagcagtac cgccttctgc
     2341 tcctccgtca gggatttaaa acgggcaata ttgttcactt cctccggcgg catggtcagg
     2401 cacacccacc actctgccat attcagcatc ttctccgaga catccggata atccttcagg
     2461 ttctgcgtgg ccagccacag ccaggccccc agcttgcgcc acattttcac cactttggtc
     2521 atatacgggg acaaaagcgg gtttaccgtc acgatatgcg cctcatccac cacaaacaga
     2581 atatcccgtc ccagatactg atcgcgctcg gcaatgttgt taatcatatt ggtcattgac
     2641 accatggtca gtgccatctg ggcttcataa ccttcacggg caagatgccc cagatcaatc
     2701 agggtcacat ccgcttccgg ccacagtttt ccttcccggt taaacagctc cgcttcaaaa
     2761 ctgcccggct gggtgaacat ccccagcgac tctgccatct cagcagcttt agcccggcga
     2821 acatcattac gctgagtgtc ccgcgaaatt tcccacaacg cactctgcag atccaccggc
     2881 agcatctgac gcccctcccg gtacgtggtg tgcgtggcca tcagcagtgc ctcacggatc
     2941 atcgccctgt ccgcacgttt gagcgctgct tcttctttcg gatcccctcc ggtgatcatc
     3001 atgcgggcag agatttccat ctcccccaga atgtcgcgtt tctcttcctc ttcatccccc
     3061 tcatcttcat caagatcggg cagtgaatgc tcatccacac tctgcagcgt ctctccctgc
     3121 tccaccagtt gatgagcatc cgcaaatgga ggaagacaca cccccgttcc cggtttgaca
     3181 ctgattttat taaccgaaag cccgagactc tcaaaataat ccgccagcag accaaacgag
     3241 ttcccggcct cagcgataaa cagacgggga cgatgaacag ccattatctg cgacaacgaa
     3301 ccacacagcg tggccgattt accggctccg gtaggcccga aaagcagcag atgtgcattc
     3361 tgggtccggt ccagcttgtt cagcggatca aaggtcagca catccccgcc acggttaaag
     3421 aaactgaatc cgggatgccc cgtaccggtt tcccttcccg tcaccggcag gagccctgcc
     3481 agatgctgta cccacatcag acgcgtgtac cactgttttt tatccgtatc aggatcaaaa
     3541 cacatgggca gcgcgcgcag ccagctgttc agcggtccga catcaaactc cgggcgcact
     3601 ggctgtaagc ccgcgcccag cagcaccgtc gtcagttcaa cccgtctgcg cttcagactc
     3661 tccatatcct ctgcccgcag cagaaacgtg atcccggcac ggtagagctt gtgccggttt
     3721 cccagatatt tcttcacggt cgccacatcc tcccgggcac gcagggattc actgttctcc
     3781 ccgaccgcat ttttcgacaa cctcgtgaag cgctcttcca gtgtgtcctg tggctggacc
     3841 acaattgtca tacacagcac cgttccctcc ggaaaggtgt ccatcagggc attcactgtc
     3901 ttttcgcccc gggcctgttc ccctgtgatg gttcccggct ccggtggtgt gcgcagtttc
     3961 tccaccacaa cagcggcatg cggaagcccg tccagccacc acacgccgtt ttccggatca
     4021 gataccggtg gggtgaacca gaggctttct gcaaaatcgg tcatcaccgg catcattccc
     4081 tccggtgtct cctgcggctc tgcacgggat gccatacggt acagaatatc cctgtccacc
     4141 cattccggcc ggggattgaa cagcctcagc agccagccat gcacctgctg cccgttcatc
     4201 cgtgtacagc ggacgcctgc gccacccagc gcccccacga cccgcgaaca gacctggttc
     4261 agcattgcca ccggcggcat cggatcgtgg ttgtttttcc cgatccaccg gtaaatcacc
     4321 atcctcgtgc gccgctgctg accacgccat ggctgacctg tgaccagcgt atccctgaac
     4381 agaccttccg gtcgggcaat ccccttcaga tggcgctcca tctccctcag ccaggcctcc
     4441 gtaaatgagg agccttctgc atgaggtttc acgtaccccc tgagctggtc aacatacgtg
     4501 tccacgtcgt tttcatcctg acagaagaac tgcaccaccc agggatgtga gtcatattca
     4561 tcaaaactgt cctgcagcgc atcctccacc gtatcccgga tttgctccag acgctctgcg
     4621 gtccgccctt ccgtagcggc cggactcacc tcatacacgg caccaaccga caccccatca
     4681 tccagcagca ggcactgctc ttcgtccaga tattccaccc atggcagaaa atcaatcacc
     4741 gacggacagg catggtaaac cttcgtctca tcctgtcggg tcatcttccc cggccttgac
     4801 agtggctttc gtacctgagg ggccactctt tctttcatat ccggagatac cgcttctccg
     4861 tcatgctgcg acaccgggga agaaacccgt ttttttgtgt ctgtaaacag ggaaaatcgc
     4921 atcacaacgc ctccgttcgt tcacccggca tggcatactg cgtccggctg taaaaaggaa
     4981 atactgtgct gtatcccgga accggtgacg taccggaagc cagatgcgga aaaacataca
     5041 tcaccatgtc aggattgggc agccgcggaa actgctgact gatctcactt tcctgtgtcc
     5101 ggctgtagct tatggctccg tgcgcatctg cctgctgctc cggcattgtc agcggacgcc
     5161 gcagggtgtc cctggccgct gtcatgtgcc gcgaatgtcc tccgcttcct ttcccctgcc
     5221 agatctccag catactctgt tcacccggag gcagaagctc ctctttcgat gtcgagcagc
     5281 cggacagaat aaataccgcc accatgacgg tcattacaga taactgtcgt ttcacgggat
     5341 ctccttacat cagtccagac caccggtacc gtggacttcc cccggcagag tgaagtcgta
     5401 acgtacccgg cgcccctgat cttcataatc aatggccagt tgacgcgtga tatgcaccgc
     5461 cagcgtctgc cccgggggaa cataaatggc atcaaacgtc tgcccgtatc gctgtttcac
     5521 ccattccgcg gtttcattca tcccgccgga cagggctttt ccaagtgccg cctctccggc
     5581 actgccggtc agtgttgatg tgatgccatt gacgttattc tgtgaggtgt actgcccctc
     5641 actcagtgca tcacccgcag cgccggctgc agacagggca aatattgtcg gcagatacgt
     5701 ggaggcattg gatttacggg ttccgctgat acacgggatc ccgtcatcat ccgaaatcca
     5761 gccaatgcct cctgtactgc tgttattttt tgcctgtgcg ttactgctgc cgttcacgtc
     5821 tgatgacggc agcgtacgca ctgtcccgtc ggcaaaaaca aacgtgatgc tgttcacctg
     5881 cccacggaca caggagagcg tccagtcacc actcgcagta ccggacacca ctgcaccttc
     5941 cacatccggc agctctattc cgtttgccgt caggttatcc ttacccacca ggatcttgaa
     6001 cggataggga tccgtcacgg ttccgttcac cggaacccgt cccagaagcg ctgtcatcgc
     6061 ccggctgcct gccagcgtcg agttctccgg cagggtatac actgcccggg tgctcgcctt
     6121 ctgatgttca ttgctgtatc ccttcacctg ctgttcataa gccgctttct gacgggtcag
     6181 tggattgtca tccagaaaag agactggaaa acgcggtccg ctcttatccc ccaccgacga
     6241 cgcttcatcc tgcacctgat cggccggtgt cacccacatc agaccatccg ttcccggtga
     6301 ggctgccgat cctgtcacgc tccccagtcc gtcaaggccc agacctgctg gaatatcgct
     6361 ctcctgtcct ccggcgccgg cgtcagcact ctcctgctga cgcttcagtt gctctgtcag
     6421 tgactgaacc tgtgtcatta acgcgttctg ttcattccgg aactggcgct gcgactgctc
     6481 ataatttttc tgcaatcctg ccactgcctc actgacctga ccggaaacat ccgtgccccg
     6541 cttgcgcagc atttcatttt ctttcagcaa cttttcattc tgtttgtcca gtaccagttg
     6601 ccgctccctc acctgattca tacgcccaat cagtgtgcgc agggtatctt caggtgtgtc
     6661 tccttccacg cccagggcct tcagttcatc cggtgacagt ccggccagcg ttcctgagct
     6721 ttccgcagac gagggctccc cggtatctcc tccacagctt tttaatcccg tcaggatccc
     6781 cgccaccacc acacagggca ccacatactt cacgatcgta ttggatttaa tctgcatgct
     6841 tcacctcccc ccgtgaagga atgcgacggg aggcaggctc cgcgataaac gcctgttccg
     6901 gccgttctgc cgtcaccaca tagagcgtgg tggtatcctc aggggtacct gctgcaccca
     6961 gccagcggtg ctggaaagtc gcggcaatga attttccctg aagtgcccgc ggatcaagga
     7021 taattttgcg gcttaccgta ctcgtcagtt tcagtgcaac aactgcacgc ccacccgcag
     7081 cccaggctgc cagtggcgtg actgtcagtg gctctgacgg atagagtgtg gtgattttcc
     7141 cgggcagatg cgcattaacc ggccggatac ccggtaccgc ttccagcgtc cgtaacggtg
     7201 cgtacagcga ctgtgccgca tagcgggtca acagaacggg taaaggctct gcatacttca
     7261 ccggctgacg gagggcctgc gagccgtcat ccccgccggg taccaccttt ttgtgttctg
     7321 cagagcgtgc agacgccact gtgccagcct gactgactgt actgacgctt ccctcataca
     7381 caatccgtac gggctctgtg ggcccttttt ctgctgctgt cacatccagc agaataattt
     7441 ctccgctctc cacatcctgc agttgcagac gcgtctgcgg aaaaacactc tctgctttca
     7501 gatagactgc cccaccggta ctctgtaccc tcaactttcc gttcagtgct ggcgggaagc
     7561 ccacccgcac atttttatct gcaaaaataa cccgctcctg tcctgccatc agggtaacgg
     7621 gtaagggaat gcgctcccat ttcatcagct catcagcccg tgcgctcagt ggtaaaacca
     7681 gcagtgacag gaccatcaca atactacggt ataaacaacg ttcagctccg ttcatcactg
     7741 attatccccc ttcccggctg ccggggaggc tgcttccagc cgctgtggtg tactgctgta
     7801 gcagtccagc gccagcccga acggattacg ttcaggatcc ccctccatgc gcaccacctt
     7861 tatcggataa cggacaagtg cccgttttac cggctcagca tgaaaatact catctgccac
     7921 cagatccagc tgtacctgcc agttatcccg cccttccacc gtcaccggat ttttgttgta
     7981 cctgcccccg tagccatgtt ccggaatttc atacaccacg cgcacacgct ccagcagctc
     8041 ccccgcatca ctgcgacggc gggcatcact ctccagaatc tcccgacagg atggcgtcag
     8101 gtaggcggag agcgcatgaa ttttcgccgg gtagtccacc tctccgttct tcggccaggc
     8161 attcagttgc tgaaagatat aaaaggcaaa ggaataaacc gttgacggcg ggacttccca
     8221 ccagggacgg gtactgccgg aacgcagatc cggcggattg tgaattgtca gtctgtccgg
     8281 tgccgtcatc cacccgatac agaccagcat cagaattccg gccagcacac cgctgaaaat
     8341 acgcagtgtc aggatatgct ggtcgcgatc ccgcagagca tgacgaaaac gactcatttt
     8401 ttccccctgc tacgggtacg gcgcagggac cagccatgcg cctcccggat aaatgttttc
     8461 cggccaccac cccatgcact gcttttcttc gccagatgct gccagagcca gttgtcaggt
     8521 tttccccgct tcagccggct gatccagacg ccaccgaacc agaccagcag caggggagtc
     8581 agcaacatcc ctgtcgggat aatcacccag ccaagtagcg gaatcagcgg taacggccag
     8641 atcacccctg ccacagcgcc tgtcagggcc gccagcccca gttccggtgt ggtaaaacca
     8701 cgaaaaaccg caggctccac gttcagacga tcgggcagaa aacgaatggt ctgcatgggg
     8761 atgtctcctt acagaatgat ggaggccgac ttgcccagca gccagataat ggcgaccagc
     8821 agaacaacac caaccaccac gatggcacca aactgccccc atccggcctt cccgtcacga
     8881 acctctgaga aggtggaaag cgccgcttcc gccaccttca caaaagcaac tgcactgagc
     8941 acaagaccaa acagcacaac accatcctgg agatagcctt tgatctgtcc gaaaagtcca
     9001 ccaccgccac cggatgttgg cggctctatc tgaggcaaat cagccagagc aggtgtgatc
     9061 ccggtacaca gaaacaccgc catggcatac caggcctggc gacagatgcg cattttttta
     9121 tgcattattt tctcctgaaa cttaaacaag acactgaaat caacccgcaa acatccagat
     9181 actgcagatc agcagcagga cgagtcggaa cataaaccag ccgaacacac catcccggac
     9241 atcaccgcta cgccagcctg cccaggcatc cgtcagactc cacgctgccc agaggaacac
     9301 gactgccagc agaaatccca gcgtgatgag atgcagcaca ctgagatcca cttcacctga
     9361 agcagcccgg aaggccgtat tctgcagggc cgtcatcgcc atcagggacg ctcccgccgg
     9421 tagtcaccgg tcacggctgc ggaagaccgg ggttgcgccc gggaaggggc cagataatgg
     9481 tcaataccgt cacgaatggt ctgcagatct gcacgggcac gcagataatc aaaaaagtag
     9541 cgttccccct gtgaagaagc ctgcagagag gccgttctgg cccgctccag cgctgcctgc
     9601 acgtggtcaa gctggcgcat tacagtggcc agttcatcct tttcggcagc ctgcgcaggt
     9661 aatgcaaagg acaaaagaaa cagcatcccc gcggcaccgg ccagacggcc ggcacggcag
     9721 gatgatgccg gaaaagaaaa catacgcacc tccgtcagag ttaaacagtg cgtacaggat
     9781 gcgtaaggca ggggactgga tcagcgataa actctatttg caaaacagaa aatttttacg
     9841 ggagaataca ctggtgtgga tgaaggcaaa caacccggaa ctcctggcat gaacgacatc
     9901 atcagtataa tcattctcct gacagtctat ctcatcggcc tgatattcat tatcctgact
     9961 atcaatgata tagacagatc atcaaaaaag agaaagtacg agaaaaaact gaaagaagcg
    10021 ataataaatg gcagtttaca aaataatgat atctgtttac tggccgaacg ctggtctgcc
    10081 agtcatgaac atatcaaaag cgcactgctg tctgttttca atgattttct gactgataac
    10141 aacttttcac atcacgtccc ccgggtcaga gaactgatca catggtacga gaaatatgcc
    10201 ccattcacag aactccctga ggatataaaa caacagttac aaaatataat cagtttaccc
    10261 gatgtcagtg agtcagaagt gcagttatta gccacgtcac ttcatgacat ttataattcc
    10321 tgtcagaaaa aagagaagct ggccagaaga ataaactatg ttctgggggc tgtcagcata
    10381 gccagtttct gcatctcgct gttccagatt ctgttcaaat aaatttattc cagcctgaat
    10441 gatttattcc tgtttattaa aacacggcat cactactgtt tcccgttaat ggcaccgcat
    10501 taacaattta aagaggaaac acatcagcat ggaatttctc cgtactgccc ttccgttctg
    10561 ggccctgttc cttctgacct gggtttgtgt tgtgtatttt ttcatttatg aagaacctcg
    10621 ttgccccccg tggatctatg acgtcatcag ggccatcact ctgttcatta ttttcgtcaa
    10681 cattgttgtc attccggcgc tgtcgttact ctgacactga cgcgcactga ccgtaagggc
    10741 gtggcttgtc tgccgacacc ctgccacaaa acaagttttg catcagggtg tccggcctct
    10801 gtggattata aatatttctt gaaggatgcc atggtcacgg tgatggccac ccccagcagc
    10861 atggctgccg gccatatcag caggttcgga tacaccgacg tcggccagga caggtaaaga
    10921 atgcagggaa tatatatcgc cggtttaata gacttttttg catggtgata aacaaagctg
    10981 gactcatacg cagcaccata ccggcgcaga tcacgacgcc ccagtccctc cacaaccgcg
    11041 atcagtatca ccatcacaaa cagcggaaca gacaacacca ggatcgtcac ccgtatcagt
    11101 gtgaccaccg tgacatatac tgtggccatc aggtactcat acagataatg gctcagccag
    11161 cgccccacat cctcactgta ctgtgacaca ctgttaccca ccacggcacc ggcattttgc
    11221 tgcttcagcc acgacagaaa acctgtttcc acaaacagcc actgatacgc cgttgctatc
    11281 cattctgtca ccgttaccga tggtgccgac agcagcagac tgcgggtgaa ttccgaagac
    11341 agccacccca gttctgtctt catcactgcc tcactgtgtg ctgccccctg ctccggccag
    11401 aaaaaagcaa tcccgatata ctcaaccagc aggctcagca gcagggagga aaacaggatc
    11461 ccgaccagca tccacggcca gccccacaac agattccaga gcagtccgtg ctgacgtggc
    11521 ggagagggtt gtgctgctct gttcacctca cgacggggtt cagccattct gctctccttc
    11581 cggcatgttc acggagggag tctcattcca ccatccctct ccggtgtggt aattacgccg
    11641 catggcctct gcaatttttg caatactctc cggcatcagc gcgtcatctg catcagttgc
    11701 aggcaggggc atacggattt tccacaactg tcccccttcc agcagagcaa aggcctgtcc
    11761 tttcggcagg gtcacgatat ctgccggctc cagcaaaggc accttcacag taccaacacg
    11821 atcctgggta ctggatgtga agtcctggcc ggtcgtgaca tctgcggtat cctgatgacc
    11881 ggaaaccagc gttttggtat aaatttccac ctgggacaac tgggtcgtca gtaactcggc
    11941 cgtccggttc tccctgaccc gcagcataat catgttatta aagttaccct gcacctgggc
    12001 cgttttggca gcactaccgg tacgggcctc aatatcagaa gaggtctgcg tgtaagccgt
    12061 cacctgcata ccggcaccgc ctcccttgtt gattaacgga atgaactcat cgcccatcag
    12121 ttcattaaac tcatcacaat gcaggttaac cggatttttg gtaccattcc ctcccggaag
    12181 accggcattc attccgtgtt tgtagatatg cccggcgaca ctcaccagat ccgcaaacat
    12241 ggagttaccc acggcactgg ccacctcact gtcactcagc gcatcaagac caatgtacac
    12301 caccgccttt ttacgtatca cctgctccca gtcaaacacg ggacgggtat cgctcatatc
    12361 caggtagtca ggtgccagca gttctgccgt ctttcctgtg gtcagttttt ccagcaacgg
    12421 caacagggat gccacaattt tgtcaaaata ggtacgatca taacgtaccg cgcttcgcag
    12481 accatccagt atcggatcat agagtttctt tccggcttca ctgctgagag ccacctcaat
    12541 ggcccagata cgcacagcat ccggctggtt ctgcatggtc cggggaacat ccgcttcccc
    12601 cagtacattc atgttattca gaatggcgtt ttccagttcc ggcatctgtt cgctgatcac
    12661 cttaccagca taacggatat aaaggtcggc aatattattc acataacgca taatcagcgt
    12721 gtaatccggg cgctccccca gtgccaccag tgcacgggca acaatgttga caaaccgcca
    12781 tgcaaactca cggaaggccg cactgttacc ttcaccggat aactgaccgg ccacacggga
    12841 agccacctcc gacacacgac caaaacgccc cacggcgtta taacgcgcag agatttccgg
    12901 ccagcccaga tgaaagatat aaagctcatc gcccctgccg gcacggtgtg cttctgccca
    12961 gacacgcagc aacaggtccg catccccttt gggatcaaag acaatggtga catctccccg
    13021 acgaatatcc tgtgtgacca gcaactccgc cagccgggtt ttcccgacac gggtggtgcc
    13081 atagacaatg gtgtgcccca cacgttcacc cagtgccagc gtcacatcct gctcatccgg
    13141 ctcaatacca tgcagggcag gatttcctcc caccggcggc aggggacgga cgggattcag
    13201 cggcgagtca cgacgaagaa gctgcccaat ccagggaaga ctgtgctccg ttttcatttc
    13261 aagccagcgc gctgtccggt acagactgcc cggctgcaga tagtgtgcaa cctccggtcg
    13321 cagcgtgtcc tgaaggcgct gggtgtgctt ctgtgtccag cggaagccgc gtcccagaaa
    13381 cagccgctga cggctgaccg gaatttgccg ggaactcatc acataacggg gcagccgacg
    13441 cagattgcga cggtaacgga tgatcttcat cccctgtcgg aaacggacca ctgccagaac
    13501 gccaaagccg gcggctgtga catagctgac cgaaggcgcg agcgccaccg cccagggcgc
    13561 tgtcacacag acaccggccg ccgttgcggc caccacggcg gtattcagtt ccacagccgg
    13621 acgcagcaga gcctcaatca cataacggtt actcatgacc gggtacctct ctgtaaaaga
    13681 cgccgccacc atgagcgcgg caacgctttc tccggtaatg ctgacagtac ctccagtctg
    13741 cgctgtaaca ccatcacctc ccgccatgcg tctgccgcat tcagcagtaa tgccttatac
    13801 accagtggcc cgtcatgtcc gccggcatgc acatcatcca ctgtcaactg cgcctctgtt
    13861 ctgaaacgat ccactttcca gcaggccagg tcttcgcggg aaacacactg ccgccggatc
    13921 tgcagacgaa gatacacatc atgccgggac aacggttctg ccaggttgtg aatttcccga
    13981 caggcagacc ggaacacctg gtctgtgtga taaatactct gggccacatt gtgaagctgt
    14041 gtgcgcccct caccctgcag gtggaacaga aagcgggcca gacagctgcg gtgattaccg
    14101 tcagcactga cataccagtg aatgccatcc gtgctgacaa aacccatccc ggcatgctgt
    14161 tgctctgcct gcgtgtaata tcccggattc ttttcaagta acgggatatt gatgtccatg
    14221 cgtttaccac gctccagcag ctccagccag ctcattccgg catactgtgg atggtcagtc
    14281 cccacaatgc ggtacacatt gatactgccc tgtcccgtcc agtagtgttc cgtaataaaa
    14341 tccgacattg ccgcaaactc tgcggtatcc catgcaacca gttgttcatg tgcccaggcc
    14401 ggagcattcg cctgtatccg ctctgtaaat gcccccttca ttcagctctt ctctcctgca
    14461 cttttgagat accgataccg tcaggccacg gcataacagc cgggctcccc ctgccttccc
    14521 cggatacggc tgcagaacca caaccacgaa acagaatgac tgacgagaca ccttccataa
    14581 acaaagcgcc cagaaacaga accaccgcca gcattttcag ttcacgaata tcttcagcaa
    14641 cagcctcccc gaaagaaatc gctgttcgcg gacattcctg ctcatcgaca tacatccctg
    14701 agacggatat gacggaggaa catttttgta ccatcacatg cccttcaggc gcatcacgcc
    14761 agcgggatga ctccgtcgca taacgctgtg tcatgcctgc ctcaccacca ttcagattgt
    14821 tccagacata cagcattacc agacatcctg ccagcattga cggccagaca atcagaagcc
    14881 ggagcagcag cataacaaac cggtacagtg tcataatcat gattctcctt tgttactcag
    14941 taaggtgcca ttcagcagtt gcaacagacg ttgtccactg atcagctcaa tatccggcca
    15001 cagtgtcagc cgtgcccggc tgatgttccc cgtacgcccc gtatggataa aaaagcccct
    15061 gcatcccttc cggcgcagca gttcgccaaa agcatcaata tgtgccggac gaatggttct
    15121 tctgtaacgt ttagcctgta tcagccagcg ttcaccgtta atcaccacct gaccatcaag
    15181 accaccatcg ccgctgtatg aggggttacg gataaccggc atcccctgtt gctcaaaagc
    15241 cagcagcaac agttcctcaa acacatacgg actgattttg cgcagatatt tcagccgccg
    15301 ggcatcaccc tgcaattcgt tcagccgctt cattacccgt gaggccgtgg cacgataacg
    15361 ctgatgccgg cgtgttctca cacacgcttt ttttcgccgg ctgcgccaca gtgccggccc
    15421 caggacaaca gccagcaggc tgagaaccag gcaggctgtc agtaccggag aagcagagag
    15481 cgcagcagaa gtggcagaca gcggcatcat ggtgcaggta cctgtgacag gccgttgtcc
    15541 gttatcagca ccgggtaatg acttatctgt aacctgcggg ccagtacaga accggatgcc
    15601 ggaaccatca ccacccccgg tgccagctca cgcagtacgc gcagactgct ctcatccgcc
    15661 acattcacta ccattcccgc cgccttccgc tccctgagta ctgctgcatt catctgcagc
    15721 catgtgcggg aatgggcatc atcgcccaca agaaaaagtg caccgatgcc cggtaactga
    15781 agccggcgcg ccacaacctc tccgggtgac atttcaggcg tactgaccgg taacattgcc
    15841 gcctctccct gcggaaaaga gggtgatggc acaactgaag cgggaaccgg tggctgccgg
    15901 tttatcgcct caaaaaaggg gctggcatcc ctgccgccca ggtctgcaat cactttcagt
    15961 tctgcgccag ctgttgtaca gaacagcagc agactcagta cataaaactg tctttctcgt
    16021 ttcatttctg ctcctcctgt ggttcagtcc agatataacg gggggatggt gtcagcatgg
    16081 cggtcgctgc cacatgcaca tcagacggat tcaggaaatt atcccggtgg agccgggcaa
    16141 gatgccgtct gaccacggcg gtataacgtg cggccggcgc accacctgcc gggtggtgat
    16201 aacacccggc cacctgcagc cagctgaccg gatggcgacg ccggcattcc tgcagaatga
    16261 ccgctgccgt attcagactg gtgtaaggat caaatgccgc ccatgtggac gggaaacgat
    16321 gtccattcca gcccagattg acctgtgcta tcccgacatc aatacgtttt gccggataat
    16381 gtttcaggaa cacctgcaat gcctgccagg cctccagacg tgtggcataa cggtaccctt
    16441 ttcctgccac atttattgtc cagggccagg gtcgggccac gtccggctga cgaaaatcac
    16501 gacgccgttg tttcagtact ccggacatcg cttccggaga catggacgtt tccgtcaggg
    16561 aaacggcata caaaagctct gcgggaatac catgggcacg ggccacccgc acatacccgt
    16621 ccggtacgtt ctgaacggca gcacgacaga caccactgca ggccaccatc agcagaaccg
    16681 tcagaatgcc gccacgcgcc atccgtcagc cccctgctgc agtaccaccg gcatttttcc
    16741 ttcaccaaaa cgcagccagc gtccggcatc atgattcagg gtgatatgac ggctgcgaac
    16801 tttctccaca ggtatgctgt gcgcatgcgc ccactgacgc agaatctcat cctttccctg
    16861 actgtcgaca aggtagatat caacctgccg tcccgtggcc agtacggagg caagacgggc
    16921 atcacaggtg ccacattcct tcgccttcac aaacagcgcc agccgtcccc ccgtatctcc
    16981 ggtaagtgct cctgtttttc ccatactgac aggcaagata ccgggataca ggcgcttcca
    17041 ggccgcattc acctcctgct gaaacgccag ctccttctct gttcgttcat attcagccct
    17101 gacccacttt tcggcccaga cacgtcgctc tgccggcgtt ctggcctcta taccgagtac
    17161 cgtcagcgga tcaagtccgg gagactgtat cccccggggg ccggacatca gtgactgata
    17221 acgccggtaa tcctcctcac tcacccccca ctgcccggcc tgctgctgaa gtccggatgt
    17281 ggtactctgc gtcaccaccg taccggtcgt ccgttgcgcc tctgacgaca tattcgtctg
    17341 cccggcagac tgagccagaa cggggcacag tgccagcaga tacagtattt ttcgcttcat
    17401 catctactcc accctgagca ccgtctgacg accattcacc cggaacgttg cttcaccatg
    17461 gccggcatac acaagcgtcc acccggccac actctctcct tcacccagca gagccacctg
    17521 agagaggctg gtataccccc ggggagccac ggctgcacgg gtactgccac cgcgcagctc
    17581 tgtcccggtc agcacaaaag gagcctgccg aactgtatga actgcgcggg atttacggga
    17641 ggacgtcacc ggagcgggct ttttctcctc cgtttttttc atccccgctt tcctgctgtc
    17701 cggctgtgac ggtgactccg gtactttctg cattttctcc accagaccgc tgagtgtatc
    17761 cagtctcagg gaaagctttt tccgggcaga ctcaatctgc tccagcatct ccggcagatt
    17821 ctccagttgt tccttccagg atgctgtcag cacgggctgc tgctccagga ctgccaggcg
    17881 cttttccata tccttcagtt gcttctccgt ctctgtcagt ttcattctca ggggcgcgag
    17941 ttctgacgca gaaagcattt ctcttctctg gccttccagg gtcgtgatcc ggctgtcaag
    18001 accggtcata agcatcagta cgccatatac cactgccccc atgaccagcc ccacagtgac
    18061 agacagtatc aacacgccgg ggaaacgact ttttcttcgc tccggcgaca taccagacgc
    18121 accctccggt atctgcggct gtttctcact cattttttca gaaaccctcc ggttgccttc
    18181 acacccggtt cacgacccac aaaaggaacg gtgaccggaa caggagccag ctggctgacc
    18241 ggtaactggt aaccgtcacg cagactgtgg cagaccaccc tctgcacatc atccacttcc
    18301 agttgccatg ccggccccgc cattaactgc agggcagtac gcagtctcag tggtcccggc
    18361 tgatactgca ccgccggcag aggctgacgg tacagcacgc cgttggccgg tccggtggcg
    18421 cagagcgtgt aaccggactg tttcagggca taacgcatgg catcggccac cgtgggtttc
    18481 agggaggagg gaatgcgaat gtcgatcacc tgagaaagtg gatcacgctg tacagcctgc
    18541 ggctctgtgc tgaccagcag gtaacggtca tagcgcacca cctccggtgc ctgagaatac
    18601 acatccgggc tggcaggctg tacattacgg tttactgtga ccgacggtac cggcatggcc
    18661 gcatcccgtt gctgtagttt ctgagtgctc tgacatcccg ccatcaggag gcagactgtc
    18721 agtccggaca ggataaagcg ttttttcatc gggtatttac ctctgcagtg ataaatcaga
    18781 ggtcaatgtg cagaagaaga cggggctggc tcaatgaaca actcattacc cgctggcgaa
    18841 aaaaaacgcc tgcagacatg caggcgcttt gttgacagga aagaaagcaa cataacaaat
    18901 atgtcacaat cctgcggtta acataaatta ccgattaccg gagagcatta cgcaaatcat
    18961 catccgagaa ctgcagtatt tgtttgattt tctctgacga tatacccatc tgctgcatgc
    19021 gacgggccga tgcgtaattc tcctgaagag ccttttctct ctcctcctgg cggcccttct
    19081 cgataccttc ctggcggccc ttctcgatac cttccaggcg gcccttctca ataccttcct
    19141 ggcggccctt ctcgatacca gccagttcca gtcgttcagc gatagtcatt agatcatcct
    19201 catgtcttgg cagacgttct gccatcgccc gtataaattc aggtgaagct gaatcacatt
    19261 ctttcaccat atagttaaac aatgtcacca cctgagtgtg tgtttcctgt atctgaatca
    19321 actgtgacaa aaagtcaaga tgaaccatca gatccacttt cttctgccgg agcttcatca
    19381 taagcatcag cgcagccatc tgcttatagc gacggatacc ctcatcatca agaagaccga
    19441 catcaatcac cctgggtggc ctgccttctc cataaagctc ccgaccaaac gccggatcat
    19501 ccagacagtc aggccagttc atgctgtatg gataaggact gggtacacca tggtaaaaaa
    19561 gcaccggtat cacaacggga gcacacgcat gtgctttcag gtggcgatgt atcaccgcca
    19621 gaacatagcg catcattcgc agggccatat acttatccgc cttgctctgg tgctctgtca
    19681 gcgtataaat gtatccatcc ctgccatctg tggttttcat actccacaga acatcacagg
    19741 caaactggcg aagatccgga tccacaaacg tggtcggctc cagttgcagg gtgttcatat
    19801 cgcaccggtt cagttgttcc gggggcaacg cgcctttcag gaaatccctc gcaacagaag
    19861 gacattccat catcgctctg aacgccgcat catgtggcgt gggggtagtg ggtgtgtttt
    19921 cattcacaac cgcctccttg aattaaaagt ggggcggtaa gccccgtggt taacatcaga
    19981 tgacatggat aatccgtcgt tcaacgccat acccacgccc cataaagggg atatcatcgt
    20041 caaagtccat cggcggcatt cccccgcttc ctgaggaagc ctgaccgttt tttcccgttg
    20101 attgttgtgc attttgtggt ggttgtcccc atcctccctg agcaggagca gatgaaacat
    20161 ttccaccacc ctgctgctga cgcggagacc ccagcatctg cagggtacca ttgacactca
    20221 caacaacttc cgtggaataa cgatcaacac cattctgctg atcgaaccac ttccgggttc
    20281 ttaactgacc ttcaatatac acctgtgaac ctttacgaag gtattcaccg gcaatctcag
    20341 ccagttttcc gtaaataaca acacggtgcc attcagtgtg ttccttcatt tcaccgctct
    20401 gtttatcacg ccaggtatca gtggtggcca gcgtcagatt tgccactgca cctccgccgt
    20461 tggggaaata acggatttcc ggatcctgtc ccagatgacc aatcagtatg actttattca
    20521 ctccacgcga tgccataaca actccttatc ctcacacaca ttcacgctga gtccagatgc
    20581 cgttttccag agcccggact gcctgctcat aatttctgaa atactcttcg gattcccggg
    20641 acacaggccc ttcgtcgcac tgagtaccaa tataataacc acgagcactg ctgaggatct
    20701 gcagaggtaa ggtatagccc gctttggcgg ccagaattcc gcttgtcggt ttcataacat
    20761 gtttccttaa agttaaacga taagaaaaca gacctccctg atggttggtc tgttttcatc
    20821 agggaggttg agttgaaaaa tacgatcaga aagaattttc tgcgtattcc cgcggggtat
    20881 tgccattctg tggcggtatg gattccgatt tttcagtctt ataaaccatg tcctggccta
    20941 ttttgatcca ctccaccttg atcaggcggg ctttcagact gacgcgttgc tcaccggcat
    21001 gttcaccccg gttaagggta aaaatgtcag tagaggggtt gctcatggta aaaccaatca
    21061 gcaccttgcg atcttcatcc accgctttct ggcagcgggc aatcagggaa gttgcctctt
    21121 taccggcaac cgtcacatca aaacgcacat aggacgggct gtccgtcgga ccactcagtg
    21181 cattcacaac acaactgaga aatgtcccgt tctgatgatt aacctgacga atgttgctga
    21241 gataccccag gcctttcagt gtcagattga agtactcagt tttagcgttt gcagaagaag
    21301 tatttgcagt cataatgata tctccattct cagtatcgga aagacagaga acatcatcat
    21361 ccctgacggg ttgtgatatt cccgtcaggg agcaggaaca gaactattcc ggctgactca
    21421 gtaacagtta aagaaaacct tcatcgtcat gactgacgat atctgctctc aaaggtgggc
    21481 agatttacca ccaccagaaa catcctggct gcacatcccc tttcaggtta cgggggtgtt
    21541 gatcacccga aggcgttcct ctcagatcaa cggaacattg gtgcccaccg aagcgggatg
    21601 tggtcacccg aaggcgctcc tctcagacca cttatggagc attggtggtc gccgaagcga
    21661 catgtagcca cccgaaggcg ctcctctcag gccacttgtg gagcattggt ggtcgccgga
    21721 gcgacgttat gtgcccggac agactgaaat cactgatgga aaaagtcaac agctaaccaa
    21781 aaacaaaaaa gcaccggcaa gccggtgctt cagggggata ccaaatcccg gtgttgattc
    21841 tcatcttctc cggagagagc gactcccggc acggttttca cggagtgcat gtgccttgta
    21901 accccgccgt gtgcataacg ggttaccgga cctctcaggg ctgtcagccc cactgtggtc
    21961 aacagggtga taccagaact cgcctgcaat tttaattcag tttgtctccg acatcaccag
    22021 atttttgttt atgagaaaaa ttaatttttc ttttcctgcc cctgctgttt ccctgctcac
    22081 cagcaattcc cctgcagtcg ggataacggg agcatcccca gaaaaaaccg ctttttccct
    22141 tccgtttcac cgtcggaccg ttacagagtg gacatgaagg acctgattcc actgacagat
    22201 taaccgtctg gttacgtccg gataacacca ggtgcctgat ccactgatgc tgtttctcca
    22261 taaacgcctc cagtgagatt ttcccccggg caacatcatc cagcgcctgt tcccataaag
    22321 ccgtcatacc cggatttgtc agagagtccg gcaatgccgc caccagttct cttcccagtt
    22381 gcgtggaacg gatatgtttt cccgctctct caatatagtt gcgcgcaaat aacgtttcca
    22441 ggatccccgc gcgtgtggct tccgttccca gtcccgcatt ctccctcagg atctttttca
    22501 gcgccggatc cgttacacca gacgaagcat tcttcatcgc agtaataaga cagccttcag
    22561 taaaaggacg gggaggtacg gttttcagct ccttctgttc agcaccatcc agagcacaga
    22621 gttctccctt tatcaaaggg ggcaatatgt ttccactttc atcactgtca ggcttttctt
    22681 catctgcatg attttccggt ggaaataact gtttccaccc gataatcaca ttaactcttc
    22741 cgcgggaacg gaataactgc cccccaatca caaatgttgc ctcagtaaca tcagcttcct
    22801 gctccggcag aaactgtgcc agatagtgct gacgaatcag ttgatatacc ttcagttcat
    22861 caccgttcag tgagctgagg tcaaaaacct gccgggtcgg aataatggca tggtgagccg
    22921 taattttttt atcattccag atacgggata cccgtcctga ttccagttca gccagttgtg
    22981 gaagcacgga cggatctgaa cgcccgacag cctcaagcac ttcagaactc tcctcatgca
    23041 tcgaggcagg cagataacca cagtctgtcc tgggataagt tgttgctttg tgagtctcat
    23101 aaagggactg tgcgatatcc agaacggtct gagcccccat gccaaacttc cgggaacagg
    23161 cttcctgcaa cgttcccagc gaaaaacaca aaggtggcgg tgttttctcc ctcttctgta
    23221 caacatcctg tacaacagca gaggactgct gacggcacag accgagaaca gcaccggccg
    23281 cctgcacatt cacacaacgt ccctcatcat cgcagtactg ctcagctgcc acccagttac
    23341 tgcggaaacg gagcccctcc ttttccagcc agacaatcac ctgccaccag gaaaccggca
    23401 caaatgtggc gatctcctga tcccgtctga ccaccattgc cagcgtgggt gtctgcaccc
    23461 ggcccactga caaaacccca gaaattccac gttcccgtgc cttcagggta tacaaccggc
    23521 tcagattcat ccctgtcagc cagtcagccc gggcacgccc cttaccggct tcactgagaa
    23581 gcgcagtctg ttcgcccggg agaatattat tcagcgcctt cctgatactg gcctcatcca
    23641 gtgcagataa ccacagacgc ctgacacgcc ctctgtactg acagtactcc agcaactctc
    23701 tggctatcac ttctccttca cgatcggcat ctgtggcaat aaccacttca gaagcgcgtt
    23761 ttaacagccc ccggatcaca ataagttgtt ttttcacctt cggcttcacc accatctgcc
    23821 attgttccgg tatcagcggc aacacatcag aacgccatgg ggaaccgaac tgttcaccgt
    23881 aagcttccgg ctcagccagt tccagcaggt gtcctatcgc ccaggtgaca gcaatattgt
    23941 catctccgat gtatccgtcg ccccgcttac tgagcgccag ttcacgggcg atatctttcg
    24001 cctgagaagg tttttcacac agaaagagtt gcatactcac cccgctttaa ccggtggtga
    24061 ataaacagaa cgtttcttac cgctcatcac gtcaggatcc ggctccccca tgcggttaac
    24121 cgcctccttt ccacgggctg tgccggacaa aatatcagca cgtgtaacac tgactgattt
    24181 ataattttga ataatcccgt aaatcctgcg taccgcatac cctccctgat tcaacaactc
    24241 atcccgtttt gtgcgggaaa taagtccgta atgaaatacc tgaaatacct tcatgaccag
    24301 tcgatcatat ccgatcagaa gccatacaca tcgataaccg accggagaac tactgtacac
    24361 gccaatattt accggcgaag aggatttaat atcagaaagg gagatctgtc ccggcaggtt
    24421 tttaaggatc ccgtcaagcc acgcaagatg cttttcaatt gtttcagttg ccttcgtcag
    24481 ggcattttcc agcctgacta acatcatatc tgcataagga ttatccctgg ctgaatctct
    24541 ggttgcctga cccgcaaacg caatagccct gggcatactg atgatgtcag gccgcttttc
    24601 ctgtttttcc tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt
    24661 atgaagggca atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc
    24721 tccggttttc tcctggggca ttgtgtttac tccaaatgat ttcatgacca gaagatagtc
    24781 cgcaggttgc gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc
    24841 gattattttt taatcgctgt tgacactggc aacaccgctc actttttaac ctctgttgcc
    24901 actggcagca ctgttcgctt tttaacctct gctgccactg gcagcactgt tcacttttta
    24961 acctctgctg ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact
    25021 gttcgctttt taacctctgc tgccactggc agcactgctc attttttaat ctctgctgcc
    25081 actggcagca ctgttcactt tttaacctct tctgccactg gcagcactgt tcgcttttta
    25141 acctctgctg ccactggcag cactgttcac tttttgacct ctactgccac tggcagcact
    25201 gctcattttt taatccctgc tgccactggc agcgctgctc attttttaat ctctgctgcc
    25261 actggcagca ctgctcattt tttaatctct gctgccactg gcagtactgc tcatttttta
    25321 atctctgctg ccactggcaa caccgctcat tttttaatca ccgttaccac tggcagcact
    25381 gttcgctttt taacctcttc tgccactggc agcgctgctc attttttaat ctctgctgcc
    25441 actggcagca ctgctcattt tttaatctct gctgccactg gcaacaccgc tcatttttta
    25501 atcaccgtta ccactggcag cactgctcat tttttaacct ctgttgccac tggcaacacc
    25561 gctcattttt taatcactgt tgccactggc aacatggtta atgatgacta ctaccttaac
    25621 gttcagagac aaaaacagca ttaatgtcgt tatccccatc acgagcaact gaatgtcggt
    25681 tatatccaga tgagacaatg accagaaaat cacaaaaaat gaaaagcatt ctgccagcag
    25741 actcaggagg aacataaatt gcaggccccc tccgactgta ctgcctgtac gcgctttata
    25801 aagactgtat gtgacccaca gccagttact taacaccagg aaaaaccacc ctgtggcaca
    25861 tacgatataa cacatactgt tatcgtactg ttcgtgcatt gttttcagcg cagtgagcag
    25921 ggtctctgtg cctgtcacca gcccccagat aaccaccact ccggcaatca cgcaggcaca
    25981 aattttagcg atcaacaggc cactttcttt catggctgca ctcagacttt cctgtttgta
    26041 tttctgcatg ctatttacca ccttgtttca cctgaccagt tcccgattct gttttctttt
    26101 taaccagtcg ctggcggcac tggttgattt ttgttcccgc gaacatagcc cgtattgcaa
    26161 tcactcagat ctcatcgcag aacatctgcg cagcgaggcc cgtagtccag ccacaatgcc
    26221 actcagacgt tctgctgaaa tcggttcagt acgtttcccc gatgaaactg attgcgcagt
    26281 agctcgcccc ctttctcctg ccagtacgga ggctggcaca acaacacgtt gtttctgctt
    26341 cggcggataa aaatttccct cccgggctcg cttcagcact gcaagcaacc agcccacagg
    26401 attctgcagc gagtggctgc ccagcatccc tgacagactt tccagaaccc cctgcgccag
    26461 ttcttccggc agcgcctgca actgactggt cagcataatg gcctcctcct ccctgatttg
    26521 ccctgtcagc gcttcaggca atacgtacgt gtttttaatc acactgtgtg tgatattacg
    26581 tacgtaatgg ttcggtttcc gaactctgtg ataaccttct gatttttctg atggttcgga
    26641 atccgaactc agtatttttt gcccctgttt ttcactgagt tcggaatccg aactcagcat
    26701 tttcaatacc gaactctgtg tttctacccc cggttcggtt tccgaacccg gctgcattct
    26761 gccctcagtc acccgctgac gggccgccat ctgtcccggt gtctgggcac tccctatccg
    26821 ggcttccagt tgactgatat ggctgtgata atgacgcatt cccggatcat tttttatttc
    26881 gcacagaata tcccgggccg tctgacttat ggtccggttc cggctcatac aggcatctgc
    26941 caccatatcc agataacgtg gatccagtat ttcagcatcc cggcacgtca ccggttcatc
    27001 gtgctgggca taaatattgc cccgtacccg ccctttctca tcacgcaccc gtttgcacag
    27061 actcagccag cccgtgatcc gcagcatcag cagcacccgg ctgacagtct ccctggaggc
    27121 ttttcctttc cccggggaag caagctgcag ttgcagttcg tcatatgtgg ggaaaactgc
    27181 accttcatta ttctgtgcat acagccgaat catcatccac gccatcttat ccagcggaga
    27241 aagtcgcgtg tccagaagca gtcgacgcgg aatggcatca tgcacatttc cggtaaacag
    27301 cagtccgctt tgtatttttc cgtcctgttg tcggcctttc tgcactatcc gctcttgcat
    27361 tttttccagc gtgtacgcaa tcaggctgtc tacgggtaat gtcattacac ttttcctttt
    27421 tgttatacgt cagacatccg tctgctggta acgggtatat cgcccatatt caccagacag
    27481 ttacgccgga gatatgccag cgcttcatca ggaacggtac attctggtct ggcttttttc
    27541 aggttactga ttaatcgttt catcagcatt actggttcat gtgcacggat ccaccgtccc
    27601 cctgtttgat cttccagaca gcgaattaac atctcagctg gcaaaagaac cccgtggttc
    27661 tcacactcct ttttcttctc tgccgcagtt aatttctgtt gcaattcaac aattgctgcc
    27721 agcgcagatt taatcgtttc actcgtcacc cgacatggat aagcacgatt cctgccttct
    27781 ttcagaagca ctgcataacc atcagagcgg tagtgataac agtttgctcc caaccagatt
    27841 tcgtaagtat cgccgcgcga ggagataccc gttctctctg caatagccat atctcattcc
    27901 cctttgatgt gaatgacagt agtgctcatt ctcctgattt cccggagcac acaaggaaca
    27961 ccaccacttc cgaccagatc gcgtgtactc ttcagggcga cacttgattc cgcccagctt
    28021 tttcctgaag gaagttcttt cacacgaaca aaaccagctg cccgaagaga ggctccggat
    28081 tcatcagccc tggtgtatgt aatgcaacgg cgatagccca tatcttttgc cgccctccag
    28141 acagcgccat aaagtgcgct gttagcgtta cgttcaccag tagtacaggt acgattgacc
    28201 tccagtgtca gcccatcgtc aaagtggcgg gcaacaggtc tgccagctgt cgccactcct
    28261 gtcaattcac ccgcatcatt ttcccgcagt acctgaaagt taatttcggc catgtcattt
    28321 gtccttgtca ttctgctttt tcccggttca gtttccggcg actggccgat acatcattaa
    28381 accccgcagt tccctgatgc catcccctca cggcatgcca taccgccgtc agcgggatat
    28441 tcatttgctc agctgccagc atcatgacat ccagtccgtc cgcactctcc acatccgtga
    28501 tgccgctctt ctgccataaa cgccacagct ctgcactttc ctcatcactc agtgtgatgc
    28561 cacgtcccgg gcgaacatca ataccggcaa tgcgacggcg cgccgccaca tccacactgg
    28621 aaagtccaaa gtaaaacgcc atcagttcaa tggagcctcc cagcgccagt gcccggtcaa
    28681 tgcgctgcag acgtttctgc tccgtccggg cctgctgcag catacgcagc aggttttcat
    28741 gattaatgtc cagccggata accgacactt cagagtttga caggtaatga atttcctcaa
    28801 tggtcagtcc ctgcagcatc tgcatttcct cccgggccag ccccagagat tcgcagcgac
    28861 gcaggtagcc gctcttgagg tccatcacca gttgcatcag cagaccgttg gttgcctgag
    28921 acaagctgtt attcatgacg cctcccgtat catcagaccc ggcaccacct gtcgggcact
    28981 ctcccccaca ccatgatgca gtgaggcatt tttcccggcc tcatacccct gccagcgggc
    29041 caggtcaccg ccgcgacagg ctttcatctc acgggagtcc accgtcacca tatcctgctg
    29101 ctccatccag agacgcatca gccggctttc ctcttctgtc acactgaatg gcatcaccac
    29161 ctgacgaatc ccctgaaccc agccatcacg aaactgagcc gcccgggcac gacgggtgct
    29221 cagtttcagt ctccggctct gtgtacggag atacgcatcc gttgcttccc ttagctgacg
    29281 gcacagaaca tcaaacgcat aagccgccac ctgaggacgt tcactgaaac cgtaaaacgt
    29341 cacacaccgg cgataccggc ctccgggcac cgcccgccag tcgtaatacg cccggcagcc
    29401 aaatgtctgt gtcaccacca tgaccagacg aaacatccac gccggaactt tttcagcatc
    29461 agaaggtgcc ccctgtgatg aagaggtctg caccgaagaa agtccggcat cctgctccgt
    29521 gataccgtgt ttcgccatca gctgttgcgc ccttgcaagc gccagcccgg cctcatgggc
    29581 attgctgtta ttacggctca gctccagcag tttcctgacc cgcctgatca gacgttcctg
    29641 atgcgacata tcactcatca ttctcatccc cctcatcgtc gtcgggtaat acactgcgct
    29701 gcagggctcg caggcggcgc agcacactca gaagacgcag cagcttcacg gcatggtcgt
    29761 catccagcag tggccacgct tccggtgcac tgccccctgt aagcaggtct ttcagactca
    29821 cggggaggac tgtccgcggt gcatcaccgg ccagccccgc cagaaacgcc actacaggag
    29881 aacatttctc cactgctgcc ccgaaaccgg cagacaggtc gctttccctg tccatggcaa
    29941 tctcttcagc acaaccggct gattcagcca gttcccaggc cagacggaac gcttcattct
    30001 gcagatgttc gatatcatcc cgccccacag caaagtgcgt caccgacgcg ggaagtgcat
    30061 cagtggttcc cggggcagta tctcctgcca taactgatgc cagtactgcc ccggcagcac
    30121 catcttccat aacatcacca ctgaagacag gcttaccacc gtacatatcc ggctgtatct
    30181 caggacgcag ggcattttca gcagaagacg ccccacagtc ccccatgcct gcgctctctt
    30241 cctgagaagg tcgactgcat ttttcagtgc gctctgtgtc agtatcagaa acggctttca
    30301 gtggccgggt aataccatgt tgctcgcgct cagtaccggg gaatatgaag actgtgcttt
    30361 ccggcatcgc ctctgtatcc gtatccggtt gacactccgg tacttcagac cggatcccgg
    30421 ataccggtgc cccgggttgt accgtactgc tgccacggac tgaaggaggc accaccaccg
    30481 gttccggctc accaaaatga tgacgacggt tccgctcttt cggatccagt tccagcaacc
    30541 accggtcata gtccagggag ggatgtggca gggcctgcaa caaatctccg ataagctcat
    30601 cgcggaacat ctccagtgac cacagttccg gcgaattaaa cttccggcag ctttccccga
    30661 acacgcggtc aaattccgca tccgtatcgg ctgcaacagc aaactgcccc catacacgtt
    30721 ctgcgtcctg acgaagtgcg aggagtgctg taacctgtgg acgccccagt cctgactcca
    30781 tcaggtcggg gatccaggga tacagatact tcagggcatc ttccattctg ctgattgtgg
    30841 agtaatgtat cggcagccct tcatgggtta acaaatcagc cagttcacga agagacactg
    30901 ttctgcccag ttgttcctca tggatttgtc ttgccttgtg gaccccctgc gccttctcaa
    30961 taaaagtcag ttcaccgcga acttcatttt cagccagatg accaatcaca cactggagtc
    31021 gacccggcca gggtttaaac agcacatgaa tacggtaaaa acgcggatcc cctgtttctt
    31081 tccagagttc cgtcagtatc tggtaacggg tgtttccccc gtcactgaag atatacacat
    31141 ccggctcact gtccggcata cgggtgactt tgggcaccgt atccagcccg cgggcatgaa
    31201 tggaattttt gatatcgtca taacgcggat tgcgcgtggt gcgcgggtta tccggattcg
    31261 gacgtaactg gtccagcgtc agaaccatgg gcgtttcccc cattgccacc acattgccgg
    31321 caccagtggt ctgacgaccg ggctgcataa tagccgcccc gagatccagt ggtttatgtg
    31381 ccatcacgca tatcctcccg gcccgccatt ctcagagggc gtaaacagtg tgtaacgacc
    31441 gtcgaacctg acccgtaccg tacccgtggg cccctgacgc tgcttgctga caatgatttc
    31501 tgcctccccc tttgccagtg agtcggggtt atagacctcg tcgcggtaaa taaacataat
    31561 caggtcagca tcctgctcca gcgccccgga atcacgcagg tcaccgttat tggggcgttt
    31621 atcagcacgc tgctccacct gccggttaag ctgggagagg gccagcaccg ggcagcccag
    31681 ctccttcccc agcgctttca gggaacggga aatttcagca atttcctgtg tgcggttttc
    31741 cagctccggc gcacgaatca gttgcagata atccaccata atcagggagg gtttaccgta
    31801 cagacgtgta taacgacggg cgcttgcacg cagtgatgaa ggcgtctgct cacagcagtc
    31861 atcaattatt atgcgctcac ccagagagcc cattcctccg tcaccaagcg ccataccgac
    31921 cgcagatgac accctcagcc agtcctcatc atccatgttg ccactgcgca aatgattcag
    31981 ttccacacgc cccagcatgg aaagcatgcg cagaatcagt tgctccttcg gcatctcaat
    32041 gctgaatacc agaacgctct cttccgttcg ccgcagtgcg gcgctgagac acatggatag
    32101 cgccagagaa gttttcccca ttgacggacg ggcagccagc aggaccaggt cacctggctg
    32161 cagaccacag gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg tcacaccacc
    32221 ggagccgagc acactttcca gttgctgcag aacggtatcg agcgcggatg aaagagaggc
    32281 ttccgtgcgc tcccggagcg tcctgttctg cgcaagcgca aacagacgcc gctctgcagc
    32341 atccagcaca tcccgtgacg tggcatttgg tgccgccaca tcggcaatca gggtgtttcc
    32401 ggtggcctgt aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat
    32461 gttggccgca gaaggtgtgt tcttgctgag ctcggccaga taagcaaaac cacccagtac
    32521 gtccagcctg ccctgatgtt ccagatgctc tgacagtgta atcaggtcca gaggctggcc
    32581 attcaccgcc agttcagaca tggcacggaa gatggcccgg tgcggagcca cataaaaatc
    32641 gtcaggactc agcatcagca ccacttcatc ccagcgttca ttctccagca tcagcccacc
    32701 cagaacggcc tgctctgctt cgtatgcaca gagaacagac atttcagcca tggcgtaccc
    32761 tccctctctg actgccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta
    32821 cagttgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc
    32881 gaccccggtc agaaggtacg gacagcattc gttcattcat aacaagttgt tttaaaggaa
    32941 aaagtttatg aatacagaaa aacaactgcc gtccggcatt gaatctgagg tgaaatacac
    33001 cctgcagttc tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa
    33061 cccctttccc ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca
    33121 gtgggttgaa ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca
    33181 cagcgttctg tataaactga aacgtcaggg tggctccctg aatgatgccg gccggcctga
    33241 atacggccgg tgcattgctc actggcacac tgacgtgttt gttgagaaaa aagcgctgcc
    33301 tgactggctg agagcattcc acactgacgt ttcgcactcg taaaaacacg ttcagcccga
    33361 cgggtcatat tttccagcca cccgtcgggg aaaatgtcag caccatcacg gggaaacaaa
    33421 atcgctttcc attcagggta tgagcgcgga atacctccgg aagcattccg gctcagacag
    33481 cgactcacag tgcaggcagc acggaatgca tatgttgcga tgctctcatc atatgttgcg
    33541 atgttctcat tattgccttt ctgccgttcc acccgctctt cagcatcaaa tgccagtgca
    33601 gcaaggcttg cccagcacat gcagttcact gaattccccg gaacgctctg cagctcccgg
    33661 cgcaccatgt tgacctcagc actgaaaaaa gcgttctcat cgccttcaag ggcagccaga
    33721 tgcagcggaa gccagacctg actgaaccat tcgcacatcc gttcccggtt actgacggtc
    33781 atcactgtct ccgggcggga accgtcctga aaaacatgtc cggtgcgtgg agaactgtat
    33841 gaaacataac caccgtgcac cgcaatgacc ggacgacctg acgccggaag ccccacacga
    33901 cacccactgg caatccgggt catcagcagg ttcagttcag ccagtaattc agggcgggtt
    33961 aagaccgccg gaaccggaga atatttcact gaggaagaca tactttcacc tccatatctc
    34021 tcagcccgcc aggtgcgtca cttccccgca gagaaccgga aacctggcta tatgaaaaaa
    34081 gggaaaaggg taccggacga acagttgtcg ggaataaaat cccggtaact gtctgtacgt
    34141 tttcagaccg gtaagtttgg cgaccctggt cagaaggtac ggacagcatc tttgcgctca
    34201 taactgtacc gaccgggata tgcattacaa agactgtcat accgccccct ccgtctttcg
    34261 ctgtttgcag gaaagaccac atgaaaaaag ctggtcatgc cgctgacgac gctggcgttc
    34321 ctgcaaaaga aaggagtcac aaccggaaca cagccccaga ccactctcag gattcacctt
    34381 cacccagacc agagcattct taccggcacc atcccgtacc ccggaataac cacagcccac
    34441 gcacaccgtc cacttcttca gtacgatgag tgcagtttcg agtgatgcgc ggagacgccg
    34501 ggaggcaata aggagcaacg gctcatggtc ataaaaaacc ccgccgttac cccctccgga
    34561 ttcaagcagc gggagtcctg caggctcccg gggacctgtt gacatttccc gtaattcgtt
    34621 ttcattccac atattgtgga aagttatccg gttcactgaa caacgccatt cctccgccag
    34681 acgctgatac aaatgctttt tcgcttctgc gcggtcagat gcatcaacaa acacatgcac
    34741 ttcctcgtgt ccgggtgctg aagccacggc acgccatatc agttttctgc ccacaggctt
    34801 ttccctccgt tttccagcag agcatcaaaa aactcacgcc actccggcaa cagttcacag
    34861 gccagactgt gcatggtgac cagcgcagaa tcgctgcaac gggcagacac tttttccaga
    34921 cgatgcaccg gctgaccggc tgcgtgcccc tgtttataaa tattcaggtc gtaaatctgt
    34981 gttgaaagaa gggtgaccgc accttcaggc agcactttgc tgtagcgacc ggcgttgata
    35041 atgcctgtca gctctgccag tgtctgtctg gccagcgccg tataatccat gcagttaacc
    35101 agggtgcgga ctttcggcag gggaataccc agttcacggt agggcataag gttttccatg
    35161 acattgaccg tgccgcggat gaactcacgc acatccggca gaaccggatt caccatcccc
    35221 accacagagt ccgtggcgga cagcacaatc atttccagca tgacagaacg tgcgccctgt
    35281 gaatccacca caatgacgtc ataggtctga aaaagcggat gctgaagcac attacgcaga
    35341 cgaaggcgac catccggcgc atgaagcatg gccgttttca gttgctcatg ggggtcgttg
    35401 gagacgatga ggtcaagccc cctgacagcc gtacgggaaa tgatgttgtc aggatgactg
    35461 aggtcagccg tgcgcatcag taattcaaaa agaccggccg gcgcttcata cgtcagcgga
    35521 aagatattgc ttgccgtggg ctgtgcatgg tctccgtcta tcagcagagt acggaggccg
    35581 gcatctgcca gaaaaccggc aagattagca gactgggttg attttccttc gccaccttta
    35641 gtggatataa ccggtaaaat tttcatatag cacactcttt aaagtatgca accaatcact
    35701 tacactacgg aaaccagcca gaatattcac ttcactacct gaatactcgc aacacgggga
    35761 ttgaaaatcc ccgtgtcctt ggttcgattc cgagtccggg caccactttc acttccagat
    35821 tcatctcccg aactccacga aactcaagta taatcacatc gttacaagca atctgaacat
    35881 cctgaactcc accgaactct actgaactct ttctcaatca agctttcatt gggggcaccg
    35941 gtgggggctc attatgttca gtcagtttca ggagccccca tatggcactt acagccagac
    36001 aggtcgaaac agccagacct aaagaaaaag actataaact ctctgacgaa cgtggtttat
    36061 atctgctggt aaaaaccacg ggagcccgct actggcggct taaataccgg atagcaggaa
    36121 aagagaaaaa actggccctc ggcgtctatc ccgacgtctc ccttgctgag gccagaatca
    36181 aacgtgacga tgcccgaaaa atcatctccg aaggtggtga cccgggcgaa aagaagcgaa
    36241 aggaaaaact cactcagaaa atctctgcca ccaatacgtt ccatgccctc gctacggaat
    36301 ggcaccagca taaatctttg tcatggtctg aaagttacgc cagaagcgta ctggaagcgc
    36361 tggataaaga tattttcccg tatctgggca aacgaagcgt tacggatatc ctcccgctgg
    36421 aaatgctgga aattctgcgc cgcatagaaa aacgtggctc gctggaaaaa ctgcgtaagg
    36481 tacgtcaata ctgtaatcag atttttcgtt atgccatcgc caccggacga gccactgtca
    36541 atccggcatc tgaactgacc agtacgctgg cggcgccaaa agctgcacac ttcccccacc
    36601 tgagagcaga tgagctccct gtttttctca ggaagctcgc tgaatatcat ggcagtcctg
    36661 ttacccgcat ggcgacaaat ctgctgcttc tgacaggcct cagaacgatt gagctacggt
    36721 ccgctgaatg gtcagaaatt gattttgata atgccctgtg gacaatccct gaaagccgca
    36781 tgaaaatgcg acgtaaacat gtcgtaccac tgtcacgaca ggtcactgac attctgcaac
    36841 aactcaaaac tttctccgga caataccggc tggttttccc gggacgttgt gatatcaaca
    36901 agccaatgag cgaagccagc atcaatatgg tgctcaaacg tatcggttac gatggcagag
    36961 caaccggtca tggttttcgt cacaccatga gtaccattct gcacgaacag gggtttaatt
    37021 ctgcctggat tgaaatgcag ttagctcatg tggataaaaa ttccatcagg ggtacctata
    37081 atcatgccct gtatattgat ggtcgccgtg aaatgatgca gtggtatgct gactacattg
    37141 attcgctttc tgagcaggta agttaagctg agaacacgtt attctgattt tcactcactt
    37201 cacgattatt cctccggcct gcaggttcgg aggattctta tttttatgtt ttattttcag
    37261 ataaagtttt tgacaaaacg tttattctgc atcagcaaaa ttatcacaaa acaacgcaat
    37321 cacagacaaa ttaacatttt tcagatgggt atattgagtg cccacaatgt aagacaggca
    37381 ggcatccggt gacatggtaa aatattaatc cagggcaaaa acaaggcttg atacacaaca
    37441 tcatgacttt tatgctctca gactatgcca ccacagattt tacaggctca gaaaatgaat
    37501 gacagattac gctttgaagt gaacgataac cagggctgtt tcgtgttccc ggacgcctgg
    37561 tttggctcgt tgctggatga gtttgacgaa ctaattgatg catacgacag agatgaaatc
    37621 agcgaaacaa gttatatcaa caaactcaga cgccttgccc gacaggagcc tgactttatt
    37681 gatgtacacg cttacctggc atgcgcattt ctggagcaga atgccccgcg taaggcactg
    37741 aacgctgctc ttaaaggact ggcagcaggt aatcgtctca tcccggaaag tttcagtggt
    37801 gaaatcatct ggatgcatcc cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt
    37861 ctcgcaaatg tgcacttaca gcgtcatcag gatgcagctc tgctcactga taaaatactg
    37921 gcatacaatc ctgaagacaa tcagggagca cgctggctac tgggaccgga actgttacgt
    37981 actggcgacc atgaacaggc acgccatgta ctgaaagcgc atgcagatga attttcacct
    38041 tactggtatg aactcggcct tctgcatttt ctgaaccggc cccttgaaac gccagacagt
    38101 ggatgtatct taaaataaga gataggcttg gatatatgtc taacactagt tctaattttg
    38161 agatgactgg aattttgctg gggcaggagc ttcgtaaacg taaaactcct caggaaaaaa
    38221 tcgccattat tcagcagaca atggaacccg gtatgacggt gtcccatgtg gcgcgtttgc
    38281 atggtattca gcccagcctg ttgtttaagt ggaaaaaaca atatctggaa ggaagtctca
    38341 ctgctgttgc tgccggagag gatgtcgttc ctgcctcaga actggctgcc gctataaaac
    38401 aaataaaaga gttacagcgc ttgctgggta agaaatctat ggtccccacc atttttgcaa
    38461 cactgatttt gattgatgtt ggcttgccta aatctatccg gcgtcattat gggcatggat
    38521 gcccgcgcct cgatgagttc cgcacctgat gagcccaaaa aaccatccgg cttcagtgag
    38581 ccattttctt gctgaggctt ttcatcaggc cgatatgccg ttcatgtcat caataatcag
    38641 tcttcgcaaa accagattgg gaactacttt aattcatgat taacctgcca gccagactgg
    38701 cgccatcgtt acctttcgaa cacagtctgc tgtgttgtga ttgcccacgc tatccgggct
    38761 aatttgttgg ccagtgcgca acatactacg ctggagtgtt ttcttgccaa caacgccctg
    38821 acccaatccg ccaaccttcc tgactgattc tcgattctct gaataaagga tcttgcgcac
    38881 tgaacgagta accgtcgcag atttttatct ccacgtttac ttatccctaa aagtgtattt
    38941 ttaccacctg tgctgtactg ccggggtacc agaccggttg aagcagcaaa gtcccgactg
    39001 cagctatatt gtttaccgtc tcccagctgc gatgacagta cgctggcggt tatcggacca
    39061 ataccgggta ttgtctgaag acgttgtcct acctcatcac gactcagttc ctgctccagt
    39121 gcgatttcaa gctcggtgat ctgtccgaca agataaatat aatgggcatg taagcgcatc
    39181 agtaactgcg ccagataggg aggaagttcg ttctctgcaa gtactgttga aagtcgtttt
    39241 accactgcga ctcctgtcgg catactgatg ccaaattcca gcaaaaaagc atgcatctga
    39301 ttgatcgttt tgactttatc actgaccaat gattccctga cccggtgaag cgcccgcatg
    39361 gcctgctgag cttctgttcg tggttgcaca aatcgcatgg agggacgaga tgccgcttcg
    39421 catatggctt ctgcatcgac aaaatcgttc ttgttgctct taacaaaagg gcgaacgaac
    39481 tgcggagaaa tcaattttgc ttcatgacct aaatcagcaa tccggcgagc cataaaatga
    39541 gctccggcgc aggcttccat cacaacggta gacgaggcac agcctgccag aaactcaata
    39601 agtttggtac gggtaaactt tttacgcagc aataccttgc ctgacttatc ttggcagtga
    39661 atgtgaaagg aatgcttgcc aagatctata ccaataagcg taatgttttg catgatggtc
    39721 ttctccatga aagaaaaaca ctctactagg gtacagctca cagggtggtg gggaccatct
    39781 cat
//
LOCUS       DAZGCQ010000041        38080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000041 DAZGCQ010000000
VERSION     DAZGCQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.041"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..863)
                     /locus_tag="ACHK07_002255"
     CDS             complement(<1..863)
                     /locus_tag="ACHK07_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8102580.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDVSKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQV"
     gene            1498..2151
                     /locus_tag="ACHK07_002256"
     CDS             1498..2151
                     /locus_tag="ACHK07_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFO8102581.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGPGKNYLF
                     FSHGAVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYIGVFLLGKVLAL
                     PAEPFMTKLFADKSIPDVSLTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYKNLLTLAELFLVGGITSAARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            2244..2501
                     /gene="pemI"
                     /locus_tag="ACHK07_002257"
     CDS             2244..2501
                     /gene="pemI"
                     /locus_tag="ACHK07_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFO8102582.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            2503..2835
                     /gene="pemK-mt"
                     /locus_tag="ACHK07_002258"
     CDS             2503..2835
                     /gene="pemK-mt"
                     /locus_tag="ACHK07_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFO8102583.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(2950..3345)
                     /locus_tag="ACHK07_002259"
     CDS             complement(2950..3345)
                     /locus_tag="ACHK07_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO8102584.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDSLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDINVLKNIIE
                     ASSENKSESIASKDDFEEQTRRNLSMLKK"
     gene            complement(3345..4304)
                     /locus_tag="ACHK07_002260"
     CDS             complement(3345..4304)
                     /locus_tag="ACHK07_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO8102585.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            4743..5645
                     /locus_tag="ACHK07_002261"
     CDS             4743..5645
                     /locus_tag="ACHK07_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO8102586.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            5642..5952
                     /locus_tag="ACHK07_002262"
                     /pseudo
     CDS             5642..5952
                     /locus_tag="ACHK07_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6029..6100
                     /locus_tag="ACHK07_002263"
                     /pseudo
     CDS             6029..6100
                     /locus_tag="ACHK07_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
     gene            complement(6182..7882)
                     /gene="ltrA"
                     /locus_tag="ACHK07_002264"
     CDS             complement(6182..7882)
                     /gene="ltrA"
                     /locus_tag="ACHK07_002264"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO8102587.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            8509..9120
                     /locus_tag="ACHK07_002265"
                     /pseudo
     CDS             8509..9120
                     /locus_tag="ACHK07_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            9121..9342
                     /locus_tag="ACHK07_002266"
     CDS             9121..9342
                     /locus_tag="ACHK07_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102588.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            9272..9790
                     /locus_tag="ACHK07_002267"
     CDS             9272..9790
                     /locus_tag="ACHK07_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8102589.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALSDGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQV
                     RILLKNNTHEND"
     gene            9835..10605
                     /locus_tag="ACHK07_002268"
     CDS             9835..10605
                     /locus_tag="ACHK07_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102590.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDMLR
                     IPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            11020..11445
                     /locus_tag="ACHK07_002269"
     CDS             11020..11445
                     /locus_tag="ACHK07_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8102591.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            11492..11911
                     /locus_tag="ACHK07_002270"
     CDS             11492..11911
                     /locus_tag="ACHK07_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8102592.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            11911..12102
                     /locus_tag="ACHK07_002271"
     CDS             11911..12102
                     /locus_tag="ACHK07_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102593.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            12466..12717
                     /locus_tag="ACHK07_002272"
     CDS             12466..12717
                     /locus_tag="ACHK07_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102594.1"
                     /translation="MRENPWLAATGTVAPPLSRRGLRSWHPGKKTPGCKEQRRFANRA
                     SRRCGGSLLIACRVPHTRMSPCRRGLIQPQPAALTVQGK"
     gene            13141..13371
                     /locus_tag="ACHK07_002273"
     CDS             13141..13371
                     /locus_tag="ACHK07_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102595.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            13423..14784
                     /locus_tag="ACHK07_002274"
     CDS             13423..14784
                     /locus_tag="ACHK07_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO8102596.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            14831..15394
                     /locus_tag="ACHK07_002275"
     CDS             14831..15394
                     /locus_tag="ACHK07_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8102597.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            15394..15642
                     /locus_tag="ACHK07_002276"
     CDS             15394..15642
                     /locus_tag="ACHK07_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102598.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLLLFFFLPL"
     gene            15546..15812
                     /locus_tag="ACHK07_002277"
     CDS             15546..15812
                     /locus_tag="ACHK07_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102599.1"
                     /translation="MSVTDDVVVPATATATATATATATALLFPAALILTTAVLSCLPG
                     APSAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            16283..16804
                     /locus_tag="ACHK07_002278"
     CDS             16283..16804
                     /locus_tag="ACHK07_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8102600.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            16862..17095
                     /locus_tag="ACHK07_002279"
     CDS             16862..17095
                     /locus_tag="ACHK07_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO8102601.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            17156..19114
                     /locus_tag="ACHK07_002280"
     CDS             17156..19114
                     /locus_tag="ACHK07_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO8102602.1"
                     /translation="MSVTKSEPKSTRKASRKLAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVCVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPVGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            19169..19603
                     /gene="psiB"
                     /locus_tag="ACHK07_002281"
     CDS             19169..19603
                     /gene="psiB"
                     /locus_tag="ACHK07_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO8102603.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            19600..20319
                     /locus_tag="ACHK07_002282"
     CDS             19600..20319
                     /locus_tag="ACHK07_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO8102604.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            20316..20630
                     /locus_tag="ACHK07_002283"
     CDS             20316..20630
                     /locus_tag="ACHK07_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO8102605.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            20780..20932
                     /locus_tag="ACHK07_002284"
                     /pseudo
     CDS             20780..20932
                     /locus_tag="ACHK07_002284"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            20877..20999
                     /locus_tag="ACHK07_002285"
     CDS             20877..20999
                     /locus_tag="ACHK07_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8102606.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(21453..21626)
                     /locus_tag="ACHK07_002286"
                     /pseudo
     CDS             complement(21453..21626)
                     /locus_tag="ACHK07_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21925..22227
                     /locus_tag="ACHK07_002287"
     CDS             21925..22227
                     /locus_tag="ACHK07_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102607.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            22383..22670
                     /locus_tag="ACHK07_002288"
     CDS             22383..22670
                     /locus_tag="ACHK07_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102608.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            22789..23610
                     /locus_tag="ACHK07_002289"
     CDS             22789..23610
                     /locus_tag="ACHK07_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8102609.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKKNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(23907..24497)
                     /locus_tag="ACHK07_002290"
     CDS             complement(23907..24497)
                     /locus_tag="ACHK07_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8102610.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVFWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            24830..25213
                     /gene="traM"
                     /locus_tag="ACHK07_002291"
     CDS             24830..25213
                     /gene="traM"
                     /locus_tag="ACHK07_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO8102611.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            25407..26054
                     /locus_tag="ACHK07_002292"
     CDS             25407..26054
                     /locus_tag="ACHK07_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFO8102612.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVIE
                     STEVNDLNLFVWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINNLHRKEM"
     gene            26173..26400
                     /gene="traY"
                     /locus_tag="ACHK07_002293"
     CDS             26173..26400
                     /gene="traY"
                     /locus_tag="ACHK07_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO8102613.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            26435..26788
                     /gene="traA"
                     /locus_tag="ACHK07_002294"
     CDS             26435..26788
                     /gene="traA"
                     /locus_tag="ACHK07_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO8102614.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            26803..27114
                     /gene="traL"
                     /locus_tag="ACHK07_002295"
     CDS             26803..27114
                     /gene="traL"
                     /locus_tag="ACHK07_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO8102615.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            27136..27702
                     /gene="traE"
                     /locus_tag="ACHK07_002296"
     CDS             27136..27702
                     /gene="traE"
                     /locus_tag="ACHK07_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO8102616.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            27689..28417
                     /gene="traK"
                     /locus_tag="ACHK07_002297"
     CDS             27689..28417
                     /gene="traK"
                     /locus_tag="ACHK07_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO8102617.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            28417..29847
                     /gene="traB"
                     /locus_tag="ACHK07_002298"
     CDS             28417..29847
                     /gene="traB"
                     /locus_tag="ACHK07_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO8102618.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            29837..30421
                     /gene="traP"
                     /locus_tag="ACHK07_002299"
     CDS             29837..30421
                     /gene="traP"
                     /locus_tag="ACHK07_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO8102619.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            30408..30602
                     /locus_tag="ACHK07_002300"
     CDS             30408..30602
                     /locus_tag="ACHK07_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO8102620.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            30614..30865
                     /gene="trbG"
                     /locus_tag="ACHK07_002301"
     CDS             30614..30865
                     /gene="trbG"
                     /locus_tag="ACHK07_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO8102621.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            30862..31377
                     /gene="traV"
                     /locus_tag="ACHK07_002302"
     CDS             30862..31377
                     /gene="traV"
                     /locus_tag="ACHK07_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO8102622.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            31512..31733
                     /gene="traR"
                     /locus_tag="ACHK07_002303"
     CDS             31512..31733
                     /gene="traR"
                     /locus_tag="ACHK07_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO8102623.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            31726..32199
                     /locus_tag="ACHK07_002304"
     CDS             31726..32199
                     /locus_tag="ACHK07_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102624.1"
                     /translation="MHKSENEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            32279..32497
                     /locus_tag="ACHK07_002305"
     CDS             32279..32497
                     /locus_tag="ACHK07_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102625.1"
                     /translation="MHTFIFLVLMKNVGGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            32525..32872
                     /locus_tag="ACHK07_002306"
     CDS             32525..32872
                     /locus_tag="ACHK07_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102626.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIIYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            33003..35633
                     /gene="traC"
                     /locus_tag="ACHK07_002307"
     CDS             33003..35633
                     /gene="traC"
                     /locus_tag="ACHK07_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO8102627.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            35630..36016
                     /gene="trbI"
                     /locus_tag="ACHK07_002308"
     CDS             35630..36016
                     /gene="trbI"
                     /locus_tag="ACHK07_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO8102628.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            36013..36645
                     /gene="traW"
                     /locus_tag="ACHK07_002309"
     CDS             36013..36645
                     /gene="traW"
                     /locus_tag="ACHK07_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO8102629.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            36642..37634
                     /gene="traU"
                     /locus_tag="ACHK07_002310"
     CDS             36642..37634
                     /gene="traU"
                     /locus_tag="ACHK07_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO8102630.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            37846..>38080
                     /locus_tag="ACHK07_002311"
     CDS             37846..>38080
                     /locus_tag="ACHK07_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102631.1"
                     /translation="MKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWFYRPWGSSK
                     ASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVS"
BASE COUNT         8966 a         9258 c        10509 g         9347 t
ORIGIN      
        1 acctgcagaa ggctgtctcc ggtattgacc atttgcccga cagtgacact cagggagtca
       61 actttcccgt cagtcaacgc ccggataatg atttcgccct ccacatcagt gttaaccagt
      121 tctttctgta actcgtaccg ttgcagttcc atctggtaga tacggttatc aaaatctgca
      181 gcctgagtct gaatctgact ctccagagtg gttatctgca gggcattctg ttcgttctgt
      241 ccgctcaggc tgagaagatt gttttgttgc tgataatata atgccacctg gttagttaac
      301 tgatctttat taatcagccc ttttgcctga tagtttctgt aattctccat attgtttttc
      361 attattttta tcccttcctc tgcacgctgt ataatatctg atgagcgacg aaacgcatct
      421 gtgtattgca gacgttgttt ttccagggta tccagcgtta tttttttact ttcttccaga
      481 cgggaaataa tgttgtccac acgaaccagc tggttttcta tatcccgacg atgattatca
      541 gtgacaatac cactacgtgt acttttactg acgtcaatca gataaacagg atcccctttt
      601 tttatcaact gtccttcatg aacaaactgc ctgacaacaa atccctgtac acctgaatat
      661 atattgacag ctcttggcca ggttgtgacc tcaccactga cattaacacg gcggctatag
      721 gtaccaacaa tgatgaacat cagaaatgcc gtaataaaca caatgcttcc cagcatgatt
      781 aaccacagtg gtattccggg aagtaatatt gcccgtccct gccacttcat ttttctgttt
      841 tctaaagcat cctggcgaaa caaataatgt ctccttgcat actattagtt tgttgtttat
      901 ctggattaat gatggaaaat aaatcgaaaa tttgcattat atgtttcaag gaaaatcata
      961 taaacataca acataggaga gttatcagga taaataatca tataatgctg agaacaatat
     1021 gataaacaat agtcttttta tttatgatga aagaatgtca ttatttatta aatgacaata
     1081 atctaactga tttaggttat tgtcagagaa tattaaagta acataactca ttcatctatt
     1141 tcctagtttc tctgtatatt aaaagagaat ggtaatcatt atcatgttaa ggtgtcatat
     1201 cgtttacata aagtcaagat atatcagtgt ggcaagcctg gcaggattct ctagggaagg
     1261 gcaggcatac tcagtgcata gctgatgcac ggatgacggc agggggagga ggctggtgac
     1321 tttcggacga ctgtagcggc ttacatcggg cttcggcggt atcatatgaa acagcggagt
     1381 gccgccttcc atgccgctgg cgcggcatat cctggtgacg atatctgaat tgttatacat
     1441 gtgtatatat gtagtaatga caaaaatagg acaagttaaa aatttacagg agatacaatg
     1501 attcagacac gtaatcaata tctgcagttt atgctggtta tgctggctgc atggggcatt
     1561 agctggggag ccagatttgt catggagcag gccgttctgc tttatgggcc aggaaaaaac
     1621 tatttgttct tcagtcatgg tgctgttctg atgtacctgc tgtgtgtttt cctggtatac
     1681 cgccgttgga tagctccgct accggtcgtt ggtcagctgc gcaacgttgg cgtaccgtgg
     1741 ctggtcggtg cgatggccgt ggtgtatatc ggtgtatttc tgctcggtaa ggtgctggct
     1801 ctgcctgctg agccatttat gacgaaactt tttgccgata agtctatacc tgacgtgagc
     1861 ctgacgttgc tgaccatctt tatccttgcc ccgttgaatg aggaaacgct gttccggggg
     1921 attatgctga acgtcttccg ttcacggtac tgctggacga tgtggctggg ggcgctgata
     1981 acgtcgttgt tgttcgtcgc cgcgcatagc cagtataaga acctgctgac actggcagag
     2041 ctgttcctgg tgggggggat tacatcagcg gccaggatca gaagtggtgg cctgctgctg
     2101 ccggtattgc tgcatatgga agcaaccacg ctgggtttac tgtttggttg aaagttatat
     2161 ttttattaaa cattgcgcgt taaagcctgg tgtgtttttt tagtggatgt tatatttaaa
     2221 tataactttt atggaggtga agaatgcata ccacccgact gaagaaggtt ggcggctcag
     2281 ttatgctgac cgttccaccg gcactgctga atgcgctgtc tctgggtaca gataatgaag
     2341 ttggcatggt cattgataat ggccggctga ttgttgagcc gcacagacgc ccgcaatatt
     2401 cactggctga gctactggca cagtgtgatc cgaatgctga aatatcagct gaagaacgag
     2461 aatggctgga tgcaccggcg actggtcagg aggaaatctg acatggaaag aggggaaatc
     2521 tggcttgtct cgcttgatcc taccgcaggt catgagcagc agggaacgcg gccggtgctg
     2581 attgtcacac cggcggcctt taatcgcgtg acccgactgc ctgttgttgt gcccgtaacc
     2641 agcggaggca attttgcccg cactgccggc tttgcggtgt cgctggatgg tgttggcata
     2701 cgtaccacag gtgttgtgcg ttgcgatcaa ccccggacaa ttgatatgaa agcccggggc
     2761 ggaaaacgac tcgaacgggt tccagagact atcatgaacg aagttcttgg ccgtctgtcc
     2821 actattctaa cttgaacatg gggtttgagg ggcaaccgga cgaaaacgtt cgatttaggg
     2881 cactaaaacc gctgctggtc cctcattata aaagaagagc aggtaataac tgaacctgct
     2941 cttcttttat tattttttta acatagacag attgcgtctg gtttgttctt cgaaatcatc
     3001 ttttgaagcg atgctctctg atttgttttc tgaagatgcc tctataatgt tttttaacac
     3061 atttatatca attgatgtgg atgtgtatcc tgttgtttgc tctataagtt ttaccagatt
     3121 ttctgcagtg attttctcac tgaaaaaacc actgattagc gttaacagtc gggggtcaac
     3181 agaataaaga gctgctccgc aaataagaga ttgtctgtag aagtctcctc gcatttgtat
     3241 cgggagagag tctattgttt gttgtgtcag gctgtcctgc gggtaaagtg tcgggtgcag
     3301 gtatgccaca actttctttc tttttttcgg atcactgctt tccattacgc gtgcaccatt
     3361 gctttcacca atgcaaactg aggttcatct accttgttca ctttcagatt cgggaaatat
     3421 tgctttatgt gagggtaaat aagctcagct cccccgcctg tgaggtagat tcggttcatt
     3481 cctgagaatg ttgaaatttc ctgcctgatg ctttctgcca gagatttcac ctcactgtct
     3541 attacatgag taatattttt tatttcagct acatcattta ttaatgtctg gagatattca
     3601 ttatcatgcc tgttttttat taactcatca gcaataaaat aattgcaggg tgtggaggct
     3661 tttgttagtg cattcatgac ggtatgagtt atgcgggatg tcccgatttc tgaatagcca
     3721 cggatttcag aaatgccttc aaatgcaccg agaattaaac cacaatccag agtggttcct
     3781 ccaagatcga taatcaggct gcgttcaagt ttgtttactt tatctttttt tagtgattca
     3841 aatgcagctg gcaaagattc aggcattaca ttaacttttt taattttaaa tgtttcccct
     3901 ttattcagag atatctcacg gagaacattt gccttttttc tttctattct ttcttcattt
     3961 ggttgattgt cattatcaaa gaattcggtt acaggtaatg tcactgtcag ttctacatcc
     4021 tgtggctgaa ggccggatgt cagcagtgca tgatgtatgg caagcagatt ggtggtgctg
     4081 tattgataac tgacatgtgt tgtacctatg acagcagtgc tgcccaaatc ataggtgtat
     4141 ttttttccat ccacgacgta attgtaaact ttgccggcat taaacagtcc cgggttccag
     4201 ccttctttaa aactgtttcc tgacagactg gttcttacct cgccatcttc aagccaggct
     4261 agttttacgt tagttgaacc atcatcgcat gatactttta gcattctttt tgcctcgttt
     4321 tgagtttgtg tttgtctcaa attgagcatc ttttatgtcg atgcatcttt ttgagttttt
     4381 gttatttctt attgtgtctc attttgggtt aatgtcaagt agcgtggctc ttttagagtc
     4441 tccttgtgtt cttgctgtga ctcaatttga gttgttattg gtttctcatt ttgcgtcaca
     4501 aactgagacg aaaactactt aaccaggccg tgccggcacg ttaaccgggc tgcatccgat
     4561 gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcacg tttcgtcgtt cctcctccac
     4621 gcgctgcggc ttcggggccg cacctgcatt cgtatgcggt cgcccggtta caggtgcggc
     4681 acggcctgat ggaggccgca tgtgagagga gaattcccat gccaaactgg tgttcgaatc
     4741 gtatgtattt ttctggtgaa ccggcacaga tcgctgagat taaacgactg gccagcggtg
     4801 cagtcacacc gctttatcgc cgcgccacaa atgaaggtat tcagctgttt ctggccggaa
     4861 gtgccggact tctgcagacc actgaagatg tgtggtttga accgtgcccc ggactgacgg
     4921 ctgccggacg tggcgttgta tcgccggaga atatcgcgtt cacccgctgg ctgacacacc
     4981 tgcagaacgg tgtactgctg gatgagcaaa actgcctgat gctgcatgaa ctctggctgc
     5041 agagcggtac tggccagcgt cgctgggaag gattaccgga cgatgtcagg gataccatca
     5101 ccgcgctttt caccgcaaaa agaggtgact ggtgtggctt ctggagtaac gaggatgtat
     5161 cggtgtggtg gaaccgtctg tgtgacaacg tactgccgga aaaaaccatg ccgtttgacc
     5221 tgctgacggt tctgcccacc cgccttgatg ttgaagtgaa tggctttaac ggtggtgttc
     5281 tgaacggtgt tccttctgca tatcactggt ataccgaaca gtatggcgtg aagtggcctt
     5341 gtgggtatga cctgaatatc agcagtcagg gagacaactt cattcaggtt gatttcgaca
     5401 cgccgtggtg tcagccggaa agcgacgtta ttgcagaatt aagccgccgt ttcagctgca
     5461 cgctggagca ctggtatgcc gaacagggct gtgatttctg tggctggcag ttgtatgagc
     5521 gcggagagct cgttgatgtg ctgtgggggg agctggaatg gtcttccccg acagatgacg
     5581 atgagctgcc ggaagtcacc ggacctgcgt ggatagtcga caatgtggcg cattatggcg
     5641 gatgaagtat gacggagacg ggcgggcaac cgcccgtttc ttttccgaca aaggatgtcg
     5701 ccgtgctcct ctttttactg cgccggctga cgcggcgcgg caggaacgct gcctgtggtc
     5761 agtgtcccgc gtccggcggg cacgggacgg gcgcttttac cgctcccggc tggcgtcggt
     5821 gacagtgtac gccagcccgt cgcccttttc tgatgaacgc ccttcaagcc gctttcgcgg
     5881 cataaccttg ccgtcagaaa gacgacggca aggtattcca cggtcggcct gacccgttac
     5941 cggacgcggt gaacagccca caggcagcgg ggaacgggcg ccacaggggc gccctcccgg
     6001 tgctctctgt aagagaagga gtgttgttat gtcccgtttt atcctgggtg actgcgtgcg
     6061 tgttatggcc acgtttccgg gcaatgctgt cgatttcatg tcgggtagca gctgcacatt
     6121 actgtgcagc cgccccctaa gaaccgtacg tgccagtttc ccggcatacg gctcaagcct
     6181 ttcaataact ccacattaga gccggcttca taacagatat tccctgatgt tttgcagtgt
     6241 gatgacatgc cggatgtaac atggccagat tacctatgtt atccagtccc ccttcggatt
     6301 tcggcacaat gtgtcgaagt tgccagcctg ttgtggcgtt cactggcagg tggcagacgg
     6361 ggcactgacc tccctgtcgg agccacagat ttcttaggcg agagtaaccc tgtatggatt
     6421 ccagcatcgt agcttttagc cgtgcttcaa agtacgattc gtagctggga tcaaaagggt
     6481 tagcagtgct ttttatttgt ctgtgtcttt tgactggcat atccgagatt tcatatagcg
     6541 ccagtaactc acgtttgcct gatggcagga gcttccctgt ttctgtgccg aatacccagt
     6601 gtcggaagtt aactgaacga aagtatttct ttcttatcca gcctgtacct ttctttgggt
     6661 gtcgtcgaca tgcccagcgc catagtgctt tccatatttc atgactgact ttctgaaaga
     6721 tcttttttga aacaacatga cgatagtatc tggcccagcc tttgaggact gggttgagat
     6781 gccatatcag tgtgctctgg ctgactgtcg gattgtcttt tatcgtctgg cggacccttt
     6841 tcaggaattc ctttaccttt tggggtgaag gtttaatcaa cagtttcccg ttatatttgc
     6901 ggatgttcat acccagaaag tcgaatccat cactgatgtg tgtgattcgt gtcttttctg
     6961 gtgagagggt aagccctctt tctgccagaa agttttctaa caagggaaga accttttctg
     7021 acagcgtgtc gtggcttatc cccgtgatca caaaatcgtc cgcataacgg attatgttga
     7081 tcttttctgg attcagtccg cctcgataga aggttttctt caacagaggc tgcagaccat
     7141 ccagaaccat gtttgccagc gttggagata ttattccgcc ctgtggagtt cctgcactgg
     7201 ttgggtataa tttccccaaa tcaatatatc cggctttcag ccatcgtttc aggatcgccg
     7261 tatccgttgg gatattggcg attagccatt catgtgaaat ctcatcaaaa caactgcgaa
     7321 tgtcggcttc cagaatccat tgtgcccgat cagccctgtg caacgttgtg aaacattgct
     7381 caatggcatc ggctgttgag cgacagggcc tgaacccaaa cgaatttggg tcagccgttg
     7441 tttctgcaac aggttccagc gccatcagat agagtgcctg catggctctg tctttcattg
     7501 tcgggatccc taatggtcgc tttttgccgt tagattttgg gatatatata cgccgtagcg
     7561 gaagaggttt gtaacccctt cttttcagcg tgtatatagc cttcatcttg ctgccgggtt
     7621 ttgaccatgt tttgccatca acacccggcg ttctcttccc tctgttatct gtcactcgtt
     7681 taactgccat tgctctggcg ctgaacgagt gggtgagtag ccattgcaag gcttttacct
     7741 tgcccgcttt ctcctcctgt attgccttca cgatacgcgc ctgcagcctt ctgacctcgc
     7801 gacggcactt ggcccagttg atattgtgcc agtgagtcac gatgtcggtg ggtgcacccg
     7861 cgtttatgtt tttcgcagtc atctgcttac cctccttcaa aggtattcag gttatctcgt
     7921 gttgaaagac catgcggaag tctgcccgct ttcgcgtgga atgatgttgc tgaaacagct
     7981 caactcctgt ccatcccatt acaggatggc attcgctttc tccgcactcc tgtacccgca
     8041 tcactgacag ctttcctcgc ggttagcctg ccctgagggc agtgatacgg gcttaccgtg
     8101 ttccgcacag tttgcaggga tgggatgggt taggttctgc ctatccaccg gtagctttga
     8161 tgtccgtgcc tccccagtca aaagaggaag gtccagctac gttcccattt tgggtcgggc
     8221 ctgtcagtgc ctttggcccg ttcagggttg acgatgttta tcagcagttc acatgtgtta
     8281 accctgccat ccagccttgc gccccatccg ccctgacact ggcagacatg cttcctcctc
     8341 gcggattcca catggtgtga aaaccgggct acgttgtccc gaaggcttca taccgaacag
     8401 ttacccgctc tgcatgcttc ggtaggctac aggcgacgga acgcctggtt ttactacttc
     8461 ctttacatga agttaaaaca acaaactgtg cgactttcac gtcgcacact gactgacccg
     8521 ccgtacctcg tcggttttcg tgaccgtttc gggcgcacca tcgctggcga taaaaccgat
     8581 gaatggctgc aaccggcctg taatgaaatg taccgcgtac tcaaaaaaga cgcgctgatg
     8641 atcagcttct acggctggca ccgtattgac cgctttatgg cggcctggaa acgcgccgga
     8701 ttcagcgtgg cagggcacct cgtgttcacc aaaaactaca catccaaatc tgcgtatgtc
     8761 gcgtaccgcc acgaatgcgc gtacatcctg gcaaagggca gtccggcccg gccccgtaac
     8821 ccgctgccgg atgtgctggg ctggaaatat tcgggcaacc gccatcaccc gacggaaaag
     8881 cccgttacca gcctgcaacc actgattgag agcttcacac atccgaacgc gattgtgctg
     8941 gacccgtttg caggcagcgg ctcaacctgc gtcgctgccc tccagtccgg acgccggtat
     9001 atcggtatcg agctgcttga acagtatcac cgtgccggac agcaacgact ggccgccgtg
     9061 caacgggcca tgcagcaggg ggccgcgaat gataactggt ttgaaccgga ggctgcgtaa
     9121 atgaactatg ccggacacga aaaacttcgc gccgacgtgg cggaggtggc caacaccatg
     9181 tgtgaccttc gggccaggct gaacgatatg gagcatcgct gtcgctttga ttccgatgtg
     9241 cttgttgaac gcctgacacg ccagacgctg tatcgcgcca atcgcctgtt catggaggcg
     9301 tataccgaaa ttctggaact ggattcctgc ttcaaagatt gagaaggaga cgagaatgta
     9361 cggaacatgc gaaacgctat gccgggagct ggcagcaaag tatccgggag acacgccact
     9421 gatgctggtt atctggtccc cggaagaaat tcaggccctc tctgacggaa tggatattgc
     9481 cctgaccgat catgaaatca ggactgtcct ggcgcgcctg gaggacatcc cggaagacca
     9541 gcggactgaa tccggtattt cttccggcgt ggcgatggag atcatcaata acgtgagcga
     9601 aaaccgccag gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca
     9661 ggcattgtgg aaacgtgaat gggccgcccg ggatcatggc ctcgccgtcc cggaatgcgt
     9721 cacccgccgt caggcggtgg ttaatcaggt ccgcatcctg ctgaaaaaca acacacacga
     9781 aaacgactga tgttatcgcc gcctccgggc ggcgatacag ggagttcatg aattatgaat
     9841 gaaacgctga atgcactgat ttatcgccac gccagcaacc tgttgctggc gcagggctgg
     9901 ccggaagaga cggatgttga ccagcgtaac ccgaagtatc cgggctggat cagcatttac
     9961 gtgcggctgg atgcgccccg gctggcgacg ttacttatca accgtcacgg cggcgtactg
    10021 ccgccgctcc ttgcctctgc cattcagaaa ctgaccggaa ccggagcgga acttgttctg
    10081 tccggcagtc agtggcagtc gctgccggta cttccggcag acggaacgca ggtgtctttc
    10141 ccgtatgccg gtgagtggct ggcagaggac gaaatcaggg ccgttcttgg tgcggtacgc
    10201 gatgcagtat gcagtgtcag ttaccaggtg gcagaagatg cgcggcgtat ccgtgcggcg
    10261 ctgaccacaa ccggtcagac gttgctgacc cgccagacgc gccgctttcg cctggtggtg
    10321 aaggaaagcg atcacccctg ctggctcgat gaagacgacg aaaacctgcc cgtggtgctc
    10381 gatgccatcc tgaaccgggg cgctcgtttt tcgtcggtgg aaatgtatct ggtcagtgag
    10441 tgtgtggagc atatcctgtc cagtgggctg gcctgcgata tgctgcgtat accggatgaa
    10501 ccgccccgcc gctggtttga ccgtggtgtt ctgcgggagg tggtccggga agcccggaac
    10561 gaaatccgca gcatggcgga tgccctggca aaaatccgga aatgatgacg ctggcgggga
    10621 aaaatctccg ccttttccag ccggtccccg gaaagagcaa aaggatgtcg cctcaggctc
    10681 cgctttaccc ggccatgcgg ggcatggcct tgtgggtttt cagttatgtg gcctcagcgt
    10741 cgtgtgcggg ctgtgccgtg cctccatcgt agccgggctt ctgcgggcgg gttgcaaggt
    10801 aaggtgaacc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
    10861 tcacccgctc agatttcgcg gtctcaccct ccaactcccg tcagccgtgc gcgtcaggct
    10921 gcggcttccc ttgcgccctt gcaaccctgc aggcccggca aagatggagg cacggcaccg
    10981 cccggtgtcg cggaatatgt gaacaacgga ggaaatccca tgcagtatgc gaaacctgtc
    11041 actctgaacg ttgaagagtg cgaccgtctg tcttttctgc cttacctgtt tggcctggat
    11101 tttctgtatg ccgaagcgtc tgtatacgcg ctggcgaaaa aaatgatgcc ggaatatgaa
    11161 ggcggattct ggcacttcat ccgtctgccg gacggtggcg gttacatgat gccggatggc
    11221 gaccgtttcc acatggtgaa cggtgcaaac tggtttgacc gtaccgtgag tgctgatgcc
    11281 gcaggcatca tccttacctc ccttgtgatt aaccgccagt tgtggctgta ccacgacagc
    11341 ggggatgcag ggttgacaca cctgtacagg atgcgcgatg cgcagttgtg gagccacatc
    11401 gaatttcacc ccgaatgcaa cgcgatttac gcggcacttg actgattaac ggacggggcg
    11461 gcaatgccgc ccctgtaaag gagacgagaa catgtactgt actgttaaag aaatcatccg
    11521 cgatgtactg gatacagacg tgccggacag tgaatgcgtt tttgccgtgg tgctgacccg
    11581 tggggatgtg cgccacatag cccaggactg gagtctgaca gacgatgagc tggaaaccgt
    11641 catgcagcgg ctggacgatg cttttgtata tggcgcatgt gacagagttg tttccgatat
    11701 tgtcaatgaa ctgatggagg aaaagcgcgt aaaccgtctt gtaacagtcc ctgcggtgct
    11761 gctggaaaaa gtgatggtga tggctggtag tgaaatttat cgcctccatg ctgtagggag
    11821 cgagaacggg ggcgacggtg acgcatttgt cagggaagaa cgtgagatta tgcgggtgat
    11881 gcgtcaggcg ctggacgggg agaacgggta atgaatatca gcacggaaac ccgcgaaatt
    11941 ctgcgtaatt acagggccgt gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc
    12001 accaccgcgc agattgttga tgaaatctgc gattttgtgg cgaatcagca ggcggttttc
    12061 ctcggtggtc actacatcct tcagggcagc aggcacaggt gatacagggc aggcggtgga
    12121 ggccgcctgt cagcccgcgc gaaaacccct ggcaggctgg cgcctgccgg aagggatgcg
    12181 ggccacgctg gcgcgtcgcc cgttaccctt cccgtttgtg atgtgtctga ctcaggagtg
    12241 tttctttctt atgttatgga actgttcctg atgttgttgt tgttcttttc tctgctgctc
    12301 tgattctgac tactgcagtg ttgtcctgtc tgccgggcgc accttcggct cccggctgcc
    12361 tggcggcatc cggtcgtcag gcccgactcc gacaggcagc tgctggcgca cccgccagtc
    12421 tccgccgaac ccgctggcgc ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccc
    12481 tggcttgcag cgaccggaac ggtcgcgccg cccttgtcgc gccgtggcct gcgctcctgg
    12541 catcccggca aaaagacgcc gggatgtaag gagcaacgcc gtttcgcaaa ccgcgcttca
    12601 cgtcgttgcg gcgggtccct gctcattgcc tgccgggtgc cacacacccg tatgagtccc
    12661 tgtcgccggg ggctgataca gccacaacct gccgcactga ctgtccaggg taaatgaaca
    12721 gcccacaaaa ttttttaccc agaacccggt aaaaaatttt gcgttctgcc caggacaggt
    12781 gcgtcaggcc gtggcagtga tgccccttgc ggcgaaggtt ctcaaacggg tgaagttcat
    12841 accacaggga atacagcagc aggatcccgc gaaacgtcaa aaccgcggct cccgaaactc
    12901 aacgggtgat gttctttaaa aatcaggatt tatttcaggc tccggcaata cgcatttatg
    12961 tgttctgtca gcatcctgct ggcaggtaat gagaacgcag gaacagaagg aggaatacac
    13021 cggaaaacgt cagatgcaga tatgaaaaaa gcaggtcagg gacctgctta atccacgccg
    13081 aagagactgt gcaaccagtc tttaaggcac tgttaccaac ctgtcataag gtatccattt
    13141 atgactgtaa gcattgtttc accttccgca gcggctgtaa agccccgccg tcatccccgt
    13201 ttcagacgcg aagacatccc cgcgccggaa attgaccccg tgctgaaagc cttcgggcga
    13261 cacatagccc ggagttttca ccgggggcgt ggtgtacaca tccccgccat gaaaaacacg
    13321 gctttcgggc aggtgctccg cacactcgaa ctgaaacgcg cttttaactg atgaggtgag
    13381 ccgccccgcg aggggcggca acagacggaa ggagaacatt ttatgacatt agcagcaatc
    13441 accatgaccg ccccggaagc cgccagccct gtgcagatgt accgcgcgac ctactcaccg
    13501 gatgacaaca aactgcgcct gtatgccgcg tcacgtcttg acccggagac gtataaaaaa
    13561 gtacatgatg ccggtttccg ctgggcacca gaacaggcgc tgtttgtcgc gccagcctgg
    13621 acaccgggcc gggaagacgt gcttctctca cttgccggag agattgagga tgaagacagt
    13681 acgctcgctg aacgtcagga agcacgggcg gagcggttta ccggatacag cggaaagcgg
    13741 gccagtgaat ccgcacaggc acttgatgaa gtggaaagac tggccgcgat gatcccgccc
    13801 ggtcagccca ttcttgtggg gcatcacagc gaacgccgcg cccgtcgtga tgcacagcgt
    13861 attgaaaacg gtatgaaacg tgctgtgatg ctctttgaac gtgcggaata ctgggaagag
    13921 cgggcgcggt cggcactgct tcacgcgaag tataaagaac gtccggacgt tcgctggcgt
    13981 cgtatcaaaa aaatcgaagc tgatttgcgc aaggcggaaa agaccatcgc acagtcgcag
    14041 aaatatctga cgatgtggcg ggctgaatcg ctggatctga atatggcaaa actcatcagc
    14101 agtcatgacc atatcagcgc ctgtttcccg ctggacaggt atccgcgccc ggcagaaaaa
    14161 agccagtatg aaggaagtcg atcgttatgg tcggccctgg atgatgacat catcaccacg
    14221 gagcaggccc gcgaaattgc gatccgctgt catgaacggc agattcagcg tcagcaacgc
    14281 tgggttaacc actatcagaa ccgcctgatc tatgagcgtg ccatgctgga cgaaagcggc
    14341 ggcgtggtta cccggacaca ggattttgag ccgggcgggc aggttttcag ccggggcgag
    14401 tggctgacca tcatccgcgt gaacaaaagc aacggggcgg tgagttcagt cacaacgccg
    14461 aattacagtt ttctcgggta cagcggcacg atgaaagtga cgcccgatcg catcacggac
    14521 tacaaagcac catcggcaga agaggctgcc gtcgccagtc aggccgcgaa gcgtccgccg
    14581 gtagtcaact atccggggga aggtttccgg gaaatgacaa aggcgcagtg ggccgccctg
    14641 ccccgggact gtaaggccgt gcgcagtgtg gcagagacag aagaccacgg ggcataccgc
    14701 taccgccgca caatggacaa taatttccgc ctggtgaatg tgtatatcac cgacatgaaa
    14761 atcacggaaa tcccacagaa ataaggtata tccccccggg aaatccccgg gggatgacgg
    14821 agataatgac atgcacagtc agttaagaga acgtatccgg ctgatgcgcg caaggctgga
    14881 taacgccgcg ccggttgctg aaatccgggc tgaatctcag ctttttgtga ctcctgcccc
    14941 ggtctgtgat cgcctggtga cgctggcgga aatcagcaac cgtgatcaca ttctggaacc
    15001 ctctgccggc accggggcca tactgcgggc gattcgggat accgcgccgg aggccatgtg
    15061 tgatgcggtg gagattaaca gcgggctggt ccggtatctg cgggaaaatt ttaacggtgt
    15121 cagggtacag tgtggcgact tcatggaatg gctgccggtg cagtattaca gccggatcat
    15181 tatgaatccg ccgttcagtc acgggcagga tatccggcat atcctgcggg ctttttccct
    15241 gttgcgtccg gggggcgttc tggttgccgt ctgtctcaac gggccacgcc agcaggagaa
    15301 gctgttaccg ttttctgacg tccgcgagga gctgccgcgc ggcacgtttg cttataccga
    15361 tgtcccgacg atgattattc gtttgcgtgc ctgatgacac agggctggcg ggaggataaa
    15421 tgcccgtctg tccgcgccgg gaaaaaaccg cgcgccggtg gcgctgaaag gggccgcgct
    15481 cacgcgccgc ccctttcatt acgcggacgt tctgatgggg gcttctgtct tttcagacct
    15541 gtcatttgtc tgttactgat gatgttgttg ttcctgctac tgctactgct actgctactg
    15601 ctactgctac tgctactgct cttctttttc ctgccgctct gattctgact actgcagtgt
    15661 tgtcctgtct gccgggcgcc ccttcggctc ccggctgcct ggcggcatcc ggtcgtcagg
    15721 cccgactccg acaggcagct gctggcgcac ctgccagtct ccgccgaacc cgctggcgcg
    15781 gtttcgggct gtcgctacgg tccgatgcgt gaaaaccccg ggtttgcagc gaccgttccg
    15841 gtcgcgccgc ctctgtcgcg ccgtggcctg cgctcctggc atcccggcaa aaggccgccg
    15901 ggatgtaagg cgcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg cgggtccctg
    15961 cccgttgttg tgtccggtgc cattcacctg tttaagcccc tgtcgccggg ggctgataca
    16021 gccacaacct gccgcactga ctgtccaggg taaatgaaca gcccacaaaa ttttttaccc
    16081 aaaacccggt aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc gtggcagtga
    16141 tgccccttgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg aacaaagagc
    16201 aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat gggggctgaa
    16261 aacatcaaca gaaggagaca tcatggcagt tcgtggcatt aacaaggtca ttctcgtggg
    16321 acgtctggga aaagatcctg atgtccgtta catccccaac gggggcgcag tggcaaatct
    16381 gcaggtggcc acgtcagaaa gctggcgtga caaacagacg ggggagatgc gggagcagac
    16441 agaatggcac cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg
    16501 taagggcgcg caggtctaca tcgaggggca actccgtacc cgtagctggg aagataacgg
    16561 tattacccgt tacgtcactg aaatccttgt gaaaaccacg ggcaccatgc agatgctggg
    16621 gagtgcacca cagcagaacg ctcaggcgca accggagcct cagcagaatg ggcagccaca
    16681 gagtgctgac gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc
    16741 gcagccggag cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg atattccgtt
    16801 ctgaacgggc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagaaatgag
    16861 gatgagcgaa tatttcagaa tacttcaggg actgccggac ggctccttta cccgcgaaca
    16921 ggcggaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgatc agggaacgca
    16981 ttttcgcctg gttgtccgtc aggatggcac gttgatctgg cgctcctgga attttgagga
    17041 ctgtgccggg tactggatga accggtacat cagggatttc gggatcctta agtaagagag
    17101 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
    17161 agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacttg caaaaacgca
    17221 ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc
    17281 actgattaaa tcaccgctga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagtga
    17341 actggctgat tccattaagg gagtcggcct gctgcagaat ctggtcgtgc ataccctgcc
    17401 tggtgaccgt tacggtgtcg ccgcaggtgg tcgccgactg gctgcactca acatgctggc
    17461 agagcgtggc atcattccgg ctgactggcc tgtctgtgtg aaggtcattc cgcaggaact
    17521 ggcgacggcg gcatcaatga ctgaaaacgg tcatcgtcgg gatatgcacc ctgctgaaca
    17581 gattgccgga ttccgcgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt
    17641 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggcagacc ttgcaccagt
    17701 catactggat gcgctggcag aagaccgcat caccaccgaa cactgtcagg cgctggcact
    17761 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
    17821 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
    17881 aggcaacagt aaattccgct tcgtgggggc tgatgctttc tcacctgacg aactgcgcac
    17941 cgatttgttc agcgacgacg agggtggcta tgtggactgt gttgcgctcg atgctgccct
    18001 gctggaaaaa ctccaggctg tcgctgaaca ccttcgggaa gctgaaggct gggaatggtg
    18061 cgccggacgt atggagtctg tcggtttctg ccgtgaggat gccggaacat accgttgtct
    18121 gccggagccg gaatcggtgc tgaccgaggc ggaagaagaa cgcctgaacg aactgatggc
    18181 acgttacgac gcactggaaa accagtgtga ggaatctgac ctgctggcag cagaaatgaa
    18241 gctgattgac tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagtgg
    18301 cgtggtggtg tcctggcgtt atggcaacgt atgtgtccag cgtggtgtgc agttgcgcag
    18361 cgaggatgac ccggctgccg acgctgaccg cacggaacag gtgcaggaga aagcgtcagt
    18421 ggaggaaatc agtctgccac tgctgacgaa aatgtcttca gagcgcacgc tggcagtcca
    18481 ggcggcgctg atgcaacagc cggacaaatc cctggcactg ctggcatgga cgctctgcct
    18541 gaatgtgttt ggcagcgggg cgtacagtaa accagcacaa atcagcctgg aatgtgaaca
    18601 ttattcgctg accagcgatg cgccatcggg gaaggaaggt gccgcattca tggcgctgat
    18661 ggcagaaaaa gcccgtcttg ctgccctgtt acctgtggga tggtcacggg acatgacgac
    18721 attcctgtca ctcagtcagg aggtgctgtt atccctgctc agtttctgca ccgcatgcag
    18781 ccttaacggt gtccagaccc gtgagtgtgg tcacacgtca cgcagtccgc ttgactcgct
    18841 ggagagcgcc atcggctttc acatgcgtga ctggtggcag ccgacgaaag caaacttctt
    18901 cggacacctg aaaaagccgc agattatcgc agccctgaat gatgccggac tgtccggtgc
    18961 cgcacgggac gcggagaaga tgaagaaagg cgatgccgct gaacatgcag agttccacat
    19021 gaaagacaac cgctgggtgc ctggctggat gggttcacca cgtccacaga cggatgccac
    19081 tgaacgcgcc gataacctgg ctgatgccgc ctgatgaaca accacaccgc cccgccggag
    19141 acggggcggc agcaagggag ataccgtgat gaaaactgaa ctgaccctgg atgtattaca
    19201 gaccatgagc gcacaggaat atgaagatat ccgggctgcc ggaagtggtg aacgtcgtga
    19261 gctgacacac gccgtgatgc gggagctgga tgcgccggat aactggacga tgaacggcga
    19321 gtacggcagc gagtttggcg gctttttccc cgtccaggtc cgtttcacgc ctgcccacga
    19381 acgttttcac ctggcgttat gttcaccggg tgacgtctct caggtctggg tgctggttct
    19441 ggtgaatgcc ggcggtgagc cgtttgcggt ggttcaggtg cagcggcggt ttgtaccgga
    19501 agccgtcagc cattcactgg cactggcggc gtcactggat gcgcaggggt acagcgtcag
    19561 tgacatcatc catatcctga tggcggaagg aggtcaggca tgagcgtacg ttcacaggca
    19621 ctggttcccc tcagcgcaga gcaacaggcc gcatggcggg cggtggcgga gacggaaaaa
    19681 cgccgccatc agggaaacac gctggcggaa tatccgtatg cgggggcgtt tttccgctgt
    19741 ctgaacggaa gccgctggat ttcgctgtct gacctgcgat ttttcatgcc gtccctgacc
    19801 gcagaagaac tgcacggcaa ccgtcttcag tggctgtatg ccatcgatgt gctgattgaa
    19861 acacagggcg aggtctgcct cctccccctt cccggcgatg cagcagaacg cttgttcccg
    19921 tcggtgcggt tccgtgtcag ggaacgccgt cgccataaat ctgcgctggt catgcagaag
    19981 tacagccgcc agcaggcgag ggaggcagag cagaaaaccc gtgcgtacca ggcgctggtt
    20041 gctcaggcag aaatcgaact ggcgttccat tcaccggaaa cagtcggaag ctggcatgca
    20101 cgctggagtg accgggtggc agagcatgac ctggaaaccc tgttctggca gtggggcgag
    20161 cgttttccgt cactggccgg aatggaacgc tggcagtggc aggatatgcc gttctggcag
    20221 gtcatcgcag aggcaggtat ggcggcaagg gaggccggtc atgccgtccg tgagatggag
    20281 cgatggatgg tgccgaacaa actccgggag gcggcgtgaa gtggcgttac tcactgcgct
    20341 ggaaactgcc ttatccctgt cctggcgagc atgaactggt ttcagaagtg gtggaggcgg
    20401 ggcagcctgc accggcatcc gttatgtcac gctgggttgc cggagcaggg tatgcggtct
    20461 gtctggattt tatttctgac cgcccggtgc gccgctggag cgaagaacgt aaggctgcgg
    20521 tacggcggcg gaatctggag aaacgtatca acaggcatgc accactgttt gcggatgaac
    20581 tgattgcccg tgaactggct gaacgaccgg attattttca gggaaagtga gtgtggtcag
    20641 tacgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct catgacgtga
    20701 aggtggtttg ttatcctgtc gtgtggcaga aggacaaaag ccccgtagtt aatttttcat
    20761 taacccacga ggcctctgca tgtctagtcc acgtcagtat ggcctcttac cgtgcaaatt
    20821 gcaaggagga gtaaaccatg aaactaccgg gaaacgccct tatctggtgc gtattaatcg
    20881 tgtgctgcac gttgttgata ttcacactcc tgacccggaa tcgcctgtgc gaagtccggc
    20941 tgaaggacgg atacagggaa gttacggcaa caatggctta cgaatccggc ggtaagtagc
    21001 aacctggagg cgggcgcagg cccgcctttt cagggctgat gctggtctga ctgcactgaa
    21061 gcgcctttat aaaggggctg ctggttcgcc ggtggcccct ttctccttgc tggcattgta
    21121 gcagcatgaa caaccagact gtcaggaagg ccacttccct ccgggaacgg gaatgaacgt
    21181 gcgcctgcgg cgctcgttct tttcccccgc cttctctgtt tatggccagt tgtcagaaat
    21241 gacattacct ttcccgtgat tattctcttt ttcttcctgc tctgattctg actactgcag
    21301 tgttgtcctg tctgccgggc gcaccttcgg ctcccggctg cctggcggca tccggtcgtc
    21361 aggcccgact ccgacaggca gctgctggcg cacctgccag tctccgccga acccgctggc
    21421 gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc ccgggcttgc agcgaccgtt
    21481 ccggtcgcgc cgcctctgtc gcgccgtggc ctgcgctccc ggcatcccgg caaaaagacg
    21541 ccgggatgta aggcgcaacg ccgtttcgca aaccgcgctt cacgtcgttg cggcgggtcc
    21601 ctgcccgttg ttgtgtccgg tgccattcac ctgtttaagc ccctgtcgcc gggggctgat
    21661 acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta
    21721 cccaaaaccc ggtaaaaatt ttgcgttctg cccaggacag gtgcgtcagg ccgtggcagt
    21781 gatgccccat gcggcgaagg ttctcaaaca ggtgatggtg aataccggag ggaacaaaag
    21841 gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac tcaacagatg atgggggctg
    21901 aaaacatcag atacaggagg aaacatgagc gcattttctt ttaacaccgc ttatcagcca
    21961 acaggcgatc agcagaaaga cgcaatcgca caaatcgaca tcatgcagaa tcgtgctgtt
    22021 caggcaaatc ttgcatacca gagcagcctg gatgcggaaa cgctattgaa acagttatgg
    22081 caaatcaatg atgaactggg ggaactgttg gatagcgtgg aaaatcacac gccagttatc
    22141 agaaaaaaag ccagtgattt gagcgcactg atgatgcttt tcagccaaca ggccggtcag
    22201 cctatgacta tgttgttgat ttgttaatga taatcagggg caggaaatta agcgaaagat
    22261 gctctgcccc gggtttgcag cgaccggaac ggtcacgcca cccttgtcgc gctgtggcct
    22321 gcggcggctc ccgaaactca accgatgatg ggggctgaaa accagagaga ggagaaagca
    22381 ggatgagtac ccgaaatatc cacgttaaca ccgcgtcgta cacgcttctt gttgccggga
    22441 agaaaaaaaa caccggggaa gagtgggacg tcctggaatt cagcagcctg actgagctga
    22501 aaaaatatcg caaaagccac ccggaaaaga tggccttcag ctatagctac gcgctcagcc
    22561 ggggtgtgga tacgcagttc cgccatatca acattgcgga agccgatcac ttcaaacagt
    22621 tcctgcgtca gattaagcgt accggcctcg atatccgggc gatctgctga ccggatgtga
    22681 tgaatatccc tcctgattgt tgcaatcttg ttaatgggga ccgggaggga tgatattaag
    22741 cggaaaaatt ttttattaaa tttattcttt cataaaggag tagctgttat gcgattagct
    22801 tcccgttttg gtcggtataa ttccattcgc cgtgagcgtc ctttaacgga tgatgaatta
    22861 atgcagtttg tgccttcggt attttccggt gataaacatg aatcccggag tgagcgttat
    22921 acatatattc caacaatcaa tatcatcaat aagttacgtg aagaaggttt ccagccgttc
    22981 tttgcatgtc agagtcgggt tcgggatttg ggacgccgcg aatacagtaa acatatgtta
    23041 cgtcttcgca gggaagggca tattaacggg caggaagttc ctgaaattat cctgcttaat
    23101 tcacatgatg gttcatccag ttatcagatg atcccaggaa tttttcgttt tgtctgcaca
    23161 aatggtctgg tgtgcggaaa taattttggc gaaatccgcg ttccacataa aggtgatatt
    23221 gtcgggcagg ttatcgaggg tgcgtatgaa gtgcccggtg tctttgataa ggtcactgat
    23281 aatatggagg cgatgaaaga aatacatctt aacagtgacg agcaacattt atttggcaga
    23341 gctgcactga tggtcaggta tgaagatgaa aataaaacac cggtgacgcc tgaacaaata
    23401 attactcccc gtcgttggga ggataaaaag aacgatctct ggacaacctg gcagcgggtt
    23461 caggagaata tgataaaagg tggactgtcg gggcgaagtg cctccggtaa aaataccagg
    23521 acaagaggca ttacgggtat tgatggtgat atccgaatca acaaagcatt atggatgatt
    23581 gccgaacagt tcagggagtg gaagtcatga taatataagt aaagccccga aaattttttc
    23641 ggggctttac ttatattatc attatgcggc attgatatgt cgtctgagat gcctcaattt
    23701 catcaactgg cttttcggta gtctgtaaat attgtgcccg cgaaaaatgg tctggcagga
    23761 ggctgcgcag ttaccggata tatataagat gggttcccgc tttgcggggc ttcggccagt
    23821 cagcattatc actcatatga tatttttgtg tgtggccttc catgccgcgt ttgcggcata
    23881 accggttttt taaaatgtat atcaaattaa ttcttctgcg agctgttcat ttttgaaagc
    23941 ggtttttcag atgcacgaag ctggatcccc ttgctgtttt ttatcccgct gtaaatccgg
    24001 taaacatctg atgcataggc aagacgccgc tggttctggc gttcggtttt cctgaatcct
    24061 gcgttgtatg caccaacagc ctcccagaag actccccatt ttttaaaggc tatagccaga
    24121 taataagcac cggtataaat gttcagacag ggatctgctg tcagatgttc cggtttaatt
    24181 ccgtagcggg ccagttcgtt aaaatgctgg gaatctacct gcataagtcc actgccatat
    24241 cccgtaaccg gattaatacc gatggcatta acccggttac gggattcttt ccatgatatt
    24301 gctctcagca agtcagggtc aattttataa tctctgccag ccagatcaaa gcaatcacca
    24361 gccttgcagg tcacatttat aaacatcagg cagatcgtca gaagccattt tctcattttc
    24421 tcacctctgg tgacgtcatc aggaaataat ttaacccact ccacaaaaag gctcaacagg
    24481 ttggtggttc tcaccaccaa aagcaccaca tactacgcaa aaacaagttt ttgctgattt
    24541 tcgtttaata tcattgagtt gtatttgcgg atttatattg tttaaatctg atttatttaa
    24601 aacagcgtcg ttaacgccgc tacagcaacg cgcagacacc gctttttagg ggtggtactg
    24661 actattttta taaaaaacat tattttatat taggggtgct gctagcggcg cggtgtattt
    24721 ttttatagga taccgccagg ggcgctgcta gcggtgcgtc cctgtttgca ttatgaactc
    24781 tggtgtttct aaattaactt tattttatgt tcaaaaaagg taatttctaa tggctaaggt
    24841 gaacctgtat atcagcaatg atgcttatga aaaaataaat gcgattattg agaagcgtcg
    24901 acaggaaggg gcaagagaaa aagatgtcag tttttcagca acagcttcaa tgcttcttga
    24961 actggggctt cgtgtatatg aagctcagat ggagcgtaaa gagtccgcgt ttaatcagat
    25021 tgagtttaat aaattacttc ttgaatgcgt tgtaaaaaca caatcatcag tagcgaaaat
    25081 tttgggtatt gagtctctca gtcctcatgt ttccgggaat ccaaagtttg aatatgccaa
    25141 tatggttgaa gatatccggg agaaggtgtc atctgagatg gaacgatttt ttccaaaaaa
    25201 tgatgaggaa taaaaaagaa ttaaaagttg attttattca aataacagaa tattgatcta
    25261 gttctatttg atgacaatta accacataac gagtatatta attatgtggt taatgccacg
    25321 ttaaaatttg aacttgaaaa tcgccgatgc agggagggac tagctccctg catcgactgt
    25381 ccatagaatc ctttgaggag gttcctatgt gtgcgatgga tactagagaa agagcactca
    25441 tacgtcaatc aggcctgccg gaaattattt tatccgcaaa atatccagtc tgtgtccgca
    25501 cagagtcagg aaaattcata gactcaaact ctgttttttt acactcaata aagtgtcaga
    25561 attgtgattc tgaaatatgg ttttctggta ttgatattga tacgcaaatt ttatttcaga
    25621 ctgctgaagt tgatgctttt tcgtcctcaa atggagtgag cattattaat ggtgtaatat
    25681 tgaatgatgt atcatgggat gtggtgattg aaagtaccga agtgaatgac ttgaatttgt
    25741 ttgtctggag atttttcgaa agatacgtag ttagtagctt ttttataaag ggcaaggagt
    25801 tttgttgtga atctgacaaa tatcgcttta ataagataat accgtatctt ttgggttata
    25861 gtcatgagta ttctgctcag agaatggatg tctctattga ccagtctaaa aaaatattta
    25921 tgaattttaa aaaacactat ggtttctcag acagggatga gtggttgcga tatgctataa
    25981 tttctgatgt gttattttat ctatatcaat acttcgctag taatattaac aaccttcacc
    26041 gcaaagaaat gtaagtattc ccggcactaa aagtgccggt ttttgttaac gtaatttact
    26101 cttttgttgt ttgttttgtg tgcgtcttat gtaaatattt tgactaaatc tcttttatgt
    26161 acgggaggtg tggtgatcaa aggtgcttta agagctgctc caagtagaaa ggttttactg
    26221 acccttgatg aggcaaccaa taaattatta cttggagcca gagagcgcag tggaagaacg
    26281 aaaacaaatg aagcattggt gaggttgcag gatcatttgc aacgttatcc agatttttat
    26341 aacgaagaga tattcaggga ggtgacagaa gagagagaat caacttttaa agaactttag
    26401 ttttaatctt tttacataca atccgaggta attgatgaat acttctgtta gtgttccggg
    26461 ggcttttacg cctgttaaaa agaaatcatc ttttttctct aaactgaacc tgcttcgcct
    26521 ggttcgtgct gtgatcccgg cagctgtgct gatgatgttc ttccctcaga tggccctggc
    26581 tgctggcggt caggacctca tgcagtccgg gaatgacaca gtcaaagcca cctttggtaa
    26641 ggactccagt attgttaaat gggtcgttct ggctgaagtg ctggtcgggg cggtcatgta
    26701 catgatgacc aaaaacgtca agttcctggc cggttttgcc atcatctctg tatttattgc
    26761 tgtaggtatg accgtcgttg gcctctgaca ggaaataaaa cgatgtcggg agacgagaat
    26821 aaacttaaga aatatcgttt cccggaaaca ctgaccaacc agagccgctg gtttggcctg
    26881 ccactggatg aactgatccc cgcagcgatc tgtattggct ggggtatcac aacatcgaaa
    26941 tatctgttcg gtattggtgc ggcggttctg gtttatttcg ggataaaaaa gctgaaaaaa
    27001 gggcggggca gcttctggtt acgggatctt atttactggt atatgccaac agtcctgctg
    27061 cgcggtattt ttcataatgt tcctgattcg tgtttccggc agtggattaa atagaactga
    27121 tacctggatt gttatatgga acacggtgcc cgcttaagta ccagtcgtgt actggccatt
    27181 gcttttatat ttatgtccct gctcattgtt ctcagcctga ctgttaacgt gattcagggg
    27241 gtgaataatt accgccttca gaatgaacag cgtactgccg tgacaccgat gggatttaat
    27301 atgccctttg cggtatcaca gaacagtgct gatgcctctt ttttgcagca gatggcgctg
    27361 gcgtttattg ccctgcgcct gaatgtctca ccggaaatcg tcgatgcttc tcaccaggca
    27421 cttctgcagt atatccgtcc gggcgcgcag aataagatga aagtgattct ggccgaagaa
    27481 gcaaagctca tcaaaaaaga taatgtgaat tctgcttttt tccagaccag tgttcgtgtc
    27541 tggccacaat atggtcgtgt ggaaatccgt gggattcgga aaacatggat tggtaactcc
    27601 gaacctttca ctgatatcaa acattacatt cttattctga aacgggaaaa cggggtgacc
    27661 tggctggata atttcgggga aacagacgat gagaaaaaat aagacagtaa taatatccgc
    27721 ctgcctgctt ttttccggca gcgtgctggc aggaaacggc acactggcac ctacagtggt
    27781 tccgatggtc aatggcggtc aggcaagtat tgccatcagt aataccagcc cgaatctgtt
    27841 taccgtcccc ggtgaccgga ttatcgccgt gaacagtctg gatggtgccc tgaccaataa
    27901 tgagcagact gcgtccggtg gtgtggtggt ggccaccgtc aacaaaaagc cttttacgtt
    27961 cattctggaa acagaacgtg gtcttaatct ttccattcag gccgttcccc gtgaaggcgc
    28021 cgggcgtacc attcagctgg tcagcgattt gcgcggaaca ggagaaactg ccggtgcatg
    28081 ggaaacatcc acgccttacg aatccctgct ggtttccatc agtcaggcgg ttcgtgccgg
    28141 acaactgcca gaaggctggt atcagatccc cgtgacaaag gaaaccctgc aggcccctgc
    28201 cggaatgtcc gcaaaagctg aagccgtatg gacggggaat cacctgaaga tggtgcgctt
    28261 taccgtggaa aataaaacgc tgtctgcgct gaatatccgg gaaagtgact tctggcagcc
    28321 gggaacccgg gcggtgatgt tcagccagcc tgtcagccag ttactggtcg ctgcccgtat
    28381 ggatgtgtac gtcattcgtg acggggaggg caactgatgg ccagtatcaa caccattgtg
    28441 aaacgcaagc agttcctgtg gctggggatt gtgattgtcg gatcggtatc agcaattggt
    28501 ggtgcgcttt atctgtctga tatggatatg tccgggaaca ctgatgccgt tgcagggcag
    28561 gagcctgtgc cggatatgac cggtgttgtg gatacgacct ttgatgacaa ggtgcgtcag
    28621 catgccacca cagagatgca ggtgacggcg gcacagatgc agaagcagta tgaggaaatc
    28681 cgtcgtgaga tggatgttct gaacaaacag cgtggtgatg accagcgtcg cattgaaaag
    28741 ctggggcagg agaatgctgc gctggcagag caggtgaaag ccctgggcgc gaatcccgtt
    28801 acagtgaccg gagagcctgt gccacagatg ccggcatccc ctcccggtcc ggagggggag
    28861 cctcagcccg gtaatgcgcc cgttgctttt ccaccgcagg gggcaggtgc agtgccaccg
    28921 cccaccgcgt tctatccggg caacggagcc acgccgccgc cacaggtgac gtaccagtct
    28981 gtgccggtgc cgaaccggat acagcgtaaa acgttcagct atgatgctgg cacaaagcaa
    29041 actccgtccc tgccgtacat tccgtcaggc agttttgcga aagccattct gattgaaggg
    29101 gcggatgcca atgcttctgt caccggtaat gaatccacgg tgccgatgca gctgcgtatc
    29161 accggtctgg tggaaatgcc gaacagcaaa acctatgacg caacgggatg ctttgttggg
    29221 ctggaagcct ggggggatgt gtccagtgag cgtgccatcg tgcgtacccg caatatcagc
    29281 tgcctgaagg agggtaaaac catcgatatg ccggttaaag ggcatgtcag cttccggggt
    29341 aaaaacggta tcaagggtga agtggtgatg cgtaacggca aaatcctcgg ctgggcatgg
    29401 ggcgcgggat ttgttgacgg tatcggtcag ggaatggagc gcgcttccca gccggctgtc
    29461 gggctgggtg ccactgccgc ttacggggcc ggcgatgtcc tgaaaatggg tatcggtggc
    29521 ggtgcatcga aagcggcaca gacactcagt gactactaca tcaaacgcgc tgagcagtat
    29581 cacccggtga tacccatcgg ggcgggtaat gaagtgacgg tggtgttcca ggacggtttc
    29641 cagctgaaaa ccgtggaaga gatggcgctg gagcaggctc aggggcaggt ggaagagagt
    29701 aacccgggaa gtcccgtgcc gaatccgcca tccgcagaga gtcacctgaa tggtctgaat
    29761 accgaccaga tgctgaagca actgggcgac ctgaatccgc agcagtttat ggccggtggt
    29821 caggaagggg gcaacgatgg caaataatac gtcgtccagt caggcgtgcc ataccgcccg
    29881 gtatgttgtc gcccgggttc tgcgtggact gttctggtgt ctgaaataca ccgtcattct
    29941 gccactggcc acaatggccc tgatggcgtt atttgtttta tggaaggaca acaccacacc
    30001 agggaaatta ctggttcagg aaattgactt cataagacaa acagcccctg ccgggcagtt
    30061 tcctgtaaga gaatgtttgc cttctctgcc agatcttcca ggagcagaag agaaaatctg
    30121 ccgttaccgt gctgctgatg ctgcagatta tgtcagggag actgaccggt cactgatgca
    30181 actggtcacc gcattatggg caacactggc actgatgtat gtctcgctgg cagcaatcac
    30241 cgggaaatat ctggtgcgac ccggaaaaat gaaatgtatc cgggtggtaa ccgcagatga
    30301 acatctgaag gaagtttata cggaagacgc ttccctgccg ggaaaaatca ggaaatgcca
    30361 tgcttatttc cctgataaca gaacaaacag aaacaacgga gataaaaatg aacatgcgta
    30421 atattcatgt tattacggcc ctctctgtac cggataaacg catatcagat gattttatgc
    30481 atgctgttct cagtaactgc accacaggag tacttcctgc accggagaaa ttcggttctg
    30541 agtcattgcc ggacaatttt actatgactg ctgtcggtgt gatgaagaat tcagaaatat
    30601 aaggaaccct tcgatgaata agctggtttc tgatggcagc gtcaaaaaaa taaattatcc
    30661 ggtgctgtat gagtccggaa taacacctcc gttatgtgaa gtcagtgcac cagaacctga
    30721 tgcgggtggg aaacgcattg tggcatatgt ttataaatca tcacggaata cggtttttga
    30781 aaatcctgac attgtaaaaa cctgtacggt cagggatttg aagaatgatt ttgtgaactg
    30841 cgatgagaaa ggtgagggac aatgaaacag atttctttat ttattccgct gctggggacg
    30901 ttattacttt ccggctgtgc cggaacaaac tccgactttg agtgtgatgc caccacatcc
    30961 gatacctgta tgacgatgga gcaggccaat gagaaggcca aaaaactgga gcagtcctca
    31021 gaagcaaagc cggttgcggc atcactgcag cgcctggctg aagggaactt ccggacaatg
    31081 ccggtgcaga ccgtcaccgc gacctctccg tccggcagca gacctgcggt gacggcactt
    31141 ccggagcaga aactgctggc accacgtccg ctgtttaccg ctgcccggga agtgaaaacg
    31201 gttgttccgg tcagttcagt tgcaccggta atacctcccc gtccgctaag aacgggtgag
    31261 cagacggcag cattatggat agcgccttat attgataatc aggatgttta tcatcagcca
    31321 tccagtgtgt tttttgttat taaaccgtct gcgtggggaa aaccacgtat taattaacag
    31381 gccctgaagt tgtatatcag ccggcaatgc cggcgggctt tctgtattca aaatctgcct
    31441 gtaaaacgag tgccctgtgc gtgaaaaggg atgggcggta gcgtgtcaga acatgtcggg
    31501 aggaatattc cgtgagtgat gaagccgatg aagcatattc agtaacagaa caactgacca
    31561 tgacaggaat aaaccggata cgccagaaaa taaatgttca tggtattcct gtttatctct
    31621 gtgaagcatg cggaaatcct attccggaag cccggcggaa aatatttccc ggtgtgacgt
    31681 tgtgcgttga atgtcaggcg tatcaggaaa ggcagaggaa acactatgca taaatcagaa
    31741 aatgaaaaaa tagattctat tctggatgaa catcaacaga tatatcgcag acataaccga
    31801 atagataaca tcctggcgtt ttttgcgtgt attgcatgga ttccctgtat tattgctgcg
    31861 ttcggcgttg atggtatata tctgaaaatc ctgtttgcgg tccttgcctg ctgtggtgcg
    31921 gtatgctttt ttattttctt cttcactctc ctgccggaat cactcatggt cctcagccgt
    31981 cagagcctgc ttcagcttat gcgtctgact gaagacgtac cggatgcgag acaggaactg
    32041 cttaaccgtc tgctgtccgg taaaaaactg actggtcggg atgaaaagga tatccggcgt
    32101 ctctggcagg agaaagtgga tgcaatgcag gagtctgcca cccgtcagcg tgaacaggat
    32161 accatccgga agtttactga agggaataaa tccgaatgaa aatctgctgt gttttataac
    32221 ctcataagtt aactgaagga gtattcagtg attaatattt cactggaggt aataaagaat
    32281 gcataccttt atttttcttg tgctgatgaa aaatgttggc ggtggcggaa gctggaatgt
    32341 tacccccatg ccaaatacgg acgtatgcgt gcaggttctt cggacaatcg aagcacatgg
    32401 aggcagggga tttggaaata caggggagtg tattgaagtt aaaactaatg aacctttgaa
    32461 taacgtgatg gaaacactga atggtggtgg gctatgagtg cttactgctt cgaaggaaaa
    32521 tgaaatgaag tcgatatatt taaaatattt tctgtctttt gtatttgttg gtgttatggc
    32581 tatgattgta tgtattcctt tttacatcat ttaccttgcg caacaacctg caacaccaga
    32641 acaactgaca gagattttac aggaaactcc ctgtgcagca gaagcatttc aggagacact
    32701 taattatcag tctgaacctt taacatttgg taaggcaaat aaaatcgcat cagaatgcag
    32761 aaaaagaaat gaaatggaag aggcgcgacg ggtaagagaa aacgagctca ataagatacg
    32821 cgaaaaacaa cttcaggcat taagtgatgc tgattcagca aaggaacgtt aattgtaatc
    32881 ctggtttcct gttaaaagcc tccatctctt atttgtgctg tggtttcaga aatggcactg
    32941 cgtgatatat ccacgccaca gactgatatt ttcctttttt aaacgtacta aggaatactc
    33001 atatgagtaa taacccactt gaggtcgtca ctcaggcggt caattcactg ctgaccgcac
    33061 ttaaattacc ggatgaatct gctcaggcta atcagatcct cggtgagatg aacttcccgc
    33121 agttcagccg tctgctgcca taccgtgatt acaaccagga atccggtctg ttcatgaatg
    33181 actccacaat gggctttatg ctggaagcca ttcccatcaa tggtgcgaat gagaccattg
    33241 tggaggctct cgaccatatg ctgcgcacca aactgccgcg cggtattccg ctgtgtatcc
    33301 atctgatgtc cagccagctg gttggcgagc ggattgagta cgggctgcgt gagttctcct
    33361 ggtcaggtga acaggcagaa cggtttaacg ccattacccg ggcctattac atgaaagccg
    33421 cagaaacact gtttccgctg ccggaagggt tgaacctgcc tctgaccctg cgccattacc
    33481 gggtgtttat ctcgtactgt tctccctcga agaaaaaaag ccgggccgac attctggaaa
    33541 tggaaaacct ggtgaaaatc atccgggcgt ctctgcaggg agcatatatc accacgcaga
    33601 cggtggatgc acaggctttc atcgatattg tcggggagat gattaaccat aacccggact
    33661 ccctgtaccc gaaaagacgt cagctggacc cgtattctga tttgaattat cagtgcgtgg
    33721 aggacagttt tgacctgaag gtccgggcag attacctgac gctgggactg cgtgagaacg
    33781 gcaggaacag cactgcccgc atcctgaatt tccatctggc ccgtaacccg gaaatcgcct
    33841 tcctgtggaa catggccgac aactacagca acctgctgaa cccggaactg tccatctcct
    33901 gcccgttcat tctgacgctg accctggtgg tggaagacca ggtgaaaacc cacagcgaag
    33961 ccaacctgaa gtacatggac ctggagaaaa agtcgaagac ctcctatgcc aaatggtttc
    34021 cgtccgtgga gaaagaagcg aaggagtggg gggaactgcg tcagcggctg ggctccggtc
    34081 agtcttctgt cgtgtcctac ttcctcaaca tcacagcatt ctgcaaggac aataatgaaa
    34141 cggcactgga agtggagcag gatatcctga acagcttccg taaaaacggt tttgaactga
    34201 tttcaccgcg ctttaaccac atgcgcaatt tcctgacctg cctgcccttt atggccggga
    34261 aagggttgtt taaacaactg aaagaggccg gagtggttca gcgcgcagag agctttaatg
    34321 tggccaacct gatgccgtta gtggcggata accccctgac gccggcaggt ctgctggcac
    34381 ccacctaccg taaccagctg gcgtttatcg atattttctt ccgggggatg aataacacca
    34441 actacaacat ggcggtctgt ggcacttccg gggccggtaa aaccgggctg atacagccac
    34501 ttatccgcag tgtgctggac tccgggggtt ttgctgtggt gttcgacatg ggggatggct
    34561 acaagtccct gtgtgaaaac atggggggcg tatatctgga cggtgaaacc ctgcgcttta
    34621 acccgtttgc gaacatcacc gatattgacc agtcagcgga gcgtgtccgt gaccagctgt
    34681 cggtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg ctgctgcagg
    34741 cggtcagggc ctcctggctt accagacaga accgggcgcg tattgatgac gtggtggatt
    34801 tcctgaaaaa cgcccgggat aacgaccagt atgccgagtc cccgaccatc cgcagccgtc
    34861 tggatgaaat gatagtgctg ctggaccagt acacagccaa cggtacttac ggccagtatt
    34921 ttaactctga tgaaccgtcc ctgcgggatg acgcaaaaat ggtggtgctg gagctgggtg
    34981 gactggaaga ccgtccgtcc ctgctggttg cggtgatgtt cagccttatc atttacatcg
    35041 agaaccggat gtaccgcacg ccgcgtaacc tcaagaaact gaacgtcatt gatgaggggt
    35101 ggcgtctgct ggacttcaaa aatcacaagg tcggtgagtt tattgagaaa ggttaccgta
    35161 ccgcccgccg tcatactggt gcctatatca ccatcacaca gaacatcgtc gactttgact
    35221 ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac aaaattatcc
    35281 tcaaacagag tgcgaaggag ttcgcgaaat ataaccagct gtatccggac cagttcctgc
    35341 cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg ttcagttcct
    35401 tcctgctgca ggtggaaaac cactcctcct ggcaccgtct gtttgtggac ccgctaagcc
    35461 gtgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa cgtaaagagg
    35521 ggctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg ccggagatgg
    35581 cttcgctgga agcatggctg gaagaacatg agaaatacag gagtgttgca tgaccacaac
    35641 gcaaaaaacg actgatgtga cagcccgtcg acgcagccac tggtggtgga cggtgccggg
    35701 atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca gactgaacac
    35761 gccggtaacc gtcgccttca acatgaaaca gacagtggat gcgttttttg acagcgccag
    35821 ccagaaaaaa ctgtcagaag cgcagtcgaa ggcgctggct gaccgtttta acgcggcact
    35881 ggaggccagt ctgcagacgt ggcagcagaa gcaccacgct gtcattctgg tgtcgcctgc
    35941 cgtggtacag ggggcaccgg atatcacccg tgaaatccag caggatattg cccggcgaat
    36001 gagggcagaa ccatgagatg ccgggggctg attgccctgc tggtatgggg gcagagtgtg
    36061 gctgcagctg atcttggcac ctggggggat ttgtggccgg taaaggaggc ggacatgctg
    36121 acggtgatta tgcagcgtct gacagctctg gagcagtccg gagagatggg ccggaaaatg
    36181 gatgcgttta aggagcgggt gatccgcaac agcctgcggc cccctgccgt gcccggtatc
    36241 ggtcggacgg agaaatacag cagccgcctg tttgacccgt ctgtcagact ggctgcggat
    36301 atccgggata acgaaggtcg tatcttcgcc cgccaggggg aggtgatgaa cccgctgcag
    36361 tatgtgcctt ttaaccagac actgtatttc atcaacggtg atgatcctga gcagattgaa
    36421 tggatgaagc gccagacgcc tcccacactg gagagcaaaa ttatcctggt gcagggcagt
    36481 atcccggaga tgcagaaggc tctggacagt cgtgtttact ttgatcagaa cggcgtgatc
    36541 tgccagcgtc tcggcattga tcaggtgccg gcacgggtga gtgctgtcag tggcgatcgt
    36601 ttcctgaagg tggagtttat tccggtcggg gagggaggga aatgaaacgg tgtatgtggt
    36661 tgtgtgtggt tttgctgctg actggccatg ttcccgcagc ctcagcggac gctgcctgtg
    36721 aagggcggtt tgttaacccg atcactgata tctgctggaa ctgcattttt ccactgtcgc
    36781 tgggcagcac aaaagtgagt caggggaagg tgccggatac aataaacccg tcaatgccga
    36841 tccagttctg tcctgcgcca ccgcctgtat ttaaacgtat cgggctggct attggttact
    36901 gggagccgat ggcactgacg gatgtgaccc gcagtccggg atgtatggta aatcttggaa
    36961 taacaatccc ggagctgggg aaaaccgggc agggaacggc caaaaaagac gacaagcagg
    37021 tgaacggggc attttaccat gttcactggt acaaataccc gctgacatac tggctgagta
    37081 ttatcacctc agcaggatgt ctgatgggcg gcgatatgga tatcgggtac atctcagaaa
    37141 ttgaccctac ctggacagac agcagtctga ccaccattct caacccggaa gcggtcatct
    37201 ttgcaaatcc gatagcacag ggagcctgtg cggtggatgc cattgccagt gcctttaaca
    37261 agccgctgga tatcctgttc tggtgtgccg gttcgcaggg cagtatgtat ccgtttaatg
    37321 gctgggtgag caatgaatcc agtccgttgc agtcatccct gctggtcagt gaacggatgg
    37381 cgtttaagct ccaccgtcag ggaatgataa tgaacagcat cggcgtggat gtggctgtct
    37441 gttatgaata tccgtcgccc atacttccca aagagcgctg gcgttatcag atggtgaata
    37501 tgtacccgga cagcggacag tgccatccgt tcggacgcag tgtgatgcgc tgggagacgg
    37561 gaaaaaatcc gcccaacaca aagaaaaact tcggttacct gatgtggcgt aaacgtaact
    37621 gtgtgttcct gtaatcggga aaatgtgatg cgcaacagta aagtgcaact tgtatcaatc
    37681 ctcagacagg tcagtctttc actgaacacg gagccattac gacagtttat ctctctgagg
    37741 gagattgcag aagagacgga tcacgtcgct gctcgtttgt ccggaggaaa acgggtaaca
    37801 cctgcacaaa tttatgaatt gtgtgcacag ttatggatgg ccaggatgaa ggcagttgaa
    37861 gtttatgggc gacattctga tgtggtgatg tctctggaaa gacagactga tttacttgag
    37921 gctgcaggca acgtattaaa acaacgctgg ttttaccgtc cgtggggatc atcaaaagca
    37981 tctgtgatgc tgacaggtat actggtgatc cccgtatttc tggttttatc cggattgttg
    38041 agtgcagggt atcccgggtt attatgtatt accgtatccg
//
LOCUS       DAZGCQ010000042        37747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000042 DAZGCQ010000000
VERSION     DAZGCQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.042"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2216
                     /gene="pdeF"
                     /locus_tag="ACHK07_002312"
     CDS             <1..2216
                     /gene="pdeF"
                     /locus_tag="ACHK07_002312"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO8102632.1"
                     /translation="RDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLPLALMI
                     SMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNN
                     VSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQ
                     ALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFALWLLALGALLL
                     LLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIP
                     IYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDPVVHLPNVRAL
                     NRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSG
                     NDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLG
                     ELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITG
                     MRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKM
                     PAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQ
                     HLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHL
                     ARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(2255..3796)
                     /gene="ppx"
                     /locus_tag="ACHK07_002313"
     CDS             complement(2255..3796)
                     /gene="ppx"
                     /locus_tag="ACHK07_002313"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO8102633.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3801..5867)
                     /gene="ppk1"
                     /locus_tag="ACHK07_002314"
     CDS             complement(3801..5867)
                     /gene="ppk1"
                     /locus_tag="ACHK07_002314"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO8102634.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6038..6676)
                     /gene="purN"
                     /locus_tag="ACHK07_002315"
     CDS             complement(6038..6676)
                     /gene="purN"
                     /locus_tag="ACHK07_002315"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO8102635.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6676..7713)
                     /gene="purM"
                     /locus_tag="ACHK07_002316"
     CDS             complement(6676..7713)
                     /gene="purM"
                     /locus_tag="ACHK07_002316"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO8102636.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8038..8664
                     /gene="upp"
                     /locus_tag="ACHK07_002317"
     CDS             8038..8664
                     /gene="upp"
                     /locus_tag="ACHK07_002317"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO8102637.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8750..10039
                     /gene="uraA"
                     /locus_tag="ACHK07_002318"
     CDS             8750..10039
                     /gene="uraA"
                     /locus_tag="ACHK07_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO8102638.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10134..10835
                     /gene="hda"
                     /locus_tag="ACHK07_002319"
     CDS             10134..10835
                     /gene="hda"
                     /locus_tag="ACHK07_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO8102639.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(10973..11332)
                     /gene="arsC"
                     /locus_tag="ACHK07_002320"
     CDS             complement(10973..11332)
                     /gene="arsC"
                     /locus_tag="ACHK07_002320"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO8102640.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11353..12816)
                     /gene="bepA"
                     /locus_tag="ACHK07_002321"
     CDS             complement(11353..12816)
                     /gene="bepA"
                     /locus_tag="ACHK07_002321"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO8102641.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13029..14090
                     /gene="yfgO"
                     /locus_tag="ACHK07_002322"
     CDS             13029..14090
                     /gene="yfgO"
                     /locus_tag="ACHK07_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8102642.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVIGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14128..14976)
                     /gene="focB"
                     /locus_tag="ACHK07_002323"
     CDS             complement(14128..14976)
                     /gene="focB"
                     /locus_tag="ACHK07_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO8102643.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(14998..17010)
                     /gene="hyfR"
                     /locus_tag="ACHK07_002324"
     CDS             complement(14998..17010)
                     /gene="hyfR"
                     /locus_tag="ACHK07_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO8102644.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLKKDCDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17040..17453)
                     /gene="hyfJ"
                     /locus_tag="ACHK07_002325"
     CDS             complement(17040..17453)
                     /gene="hyfJ"
                     /locus_tag="ACHK07_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO8102645.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFCCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDTSHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17446..18204)
                     /gene="hyfI"
                     /locus_tag="ACHK07_002326"
     CDS             complement(17446..18204)
                     /gene="hyfI"
                     /locus_tag="ACHK07_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO8102646.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDN
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLTGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18201..18746)
                     /gene="hyfH"
                     /locus_tag="ACHK07_002327"
     CDS             complement(18201..18746)
                     /gene="hyfH"
                     /locus_tag="ACHK07_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO8102647.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18756..20471)
                     /gene="hyfG"
                     /locus_tag="ACHK07_002328"
     CDS             complement(18756..20471)
                     /gene="hyfG"
                     /locus_tag="ACHK07_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO8102648.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTLEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20461..22041)
                     /gene="hyfF"
                     /locus_tag="ACHK07_002329"
     CDS             complement(20461..22041)
                     /gene="hyfF"
                     /locus_tag="ACHK07_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO8102649.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSIW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22046..22696)
                     /gene="hyfE"
                     /locus_tag="ACHK07_002330"
     CDS             complement(22046..22696)
                     /gene="hyfE"
                     /locus_tag="ACHK07_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO8102650.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22708..24147)
                     /gene="hyfD"
                     /locus_tag="ACHK07_002331"
     CDS             complement(22708..24147)
                     /gene="hyfD"
                     /locus_tag="ACHK07_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO8102651.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24164..25111)
                     /gene="hyfC"
                     /locus_tag="ACHK07_002332"
     CDS             complement(24164..25111)
                     /gene="hyfC"
                     /locus_tag="ACHK07_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO8102652.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25122..27140)
                     /gene="hyfB"
                     /locus_tag="ACHK07_002333"
     CDS             complement(25122..27140)
                     /gene="hyfB"
                     /locus_tag="ACHK07_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO8102653.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGTISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYLVAALVVLLIAIAV"
     gene            complement(27140..27757)
                     /gene="hyfA"
                     /locus_tag="ACHK07_002334"
     CDS             complement(27140..27757)
                     /gene="hyfA"
                     /locus_tag="ACHK07_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO8102654.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNENLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28010..28480)
                     /gene="bcp"
                     /locus_tag="ACHK07_002335"
     CDS             complement(28010..28480)
                     /gene="bcp"
                     /locus_tag="ACHK07_002335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO8102655.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FDVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28480..29052)
                     /gene="gcvR"
                     /locus_tag="ACHK07_002336"
     CDS             complement(28480..29052)
                     /gene="gcvR"
                     /locus_tag="ACHK07_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO8102656.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29198..30076
                     /gene="dapA"
                     /locus_tag="ACHK07_002337"
     CDS             29198..30076
                     /gene="dapA"
                     /locus_tag="ACHK07_002337"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO8102657.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30093..31127
                     /gene="bamC"
                     /locus_tag="ACHK07_002338"
     CDS             30093..31127
                     /gene="bamC"
                     /locus_tag="ACHK07_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO8102658.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31340..32053
                     /gene="purC"
                     /locus_tag="ACHK07_002339"
     CDS             31340..32053
                     /gene="purC"
                     /locus_tag="ACHK07_002339"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO8102659.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32221..33084
                     /gene="ypfJ"
                     /locus_tag="ACHK07_002340"
     CDS             32221..33084
                     /gene="ypfJ"
                     /locus_tag="ACHK07_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO8102660.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33099..35114
                     /gene="tmcA"
                     /locus_tag="ACHK07_002341"
     CDS             33099..35114
                     /gene="tmcA"
                     /locus_tag="ACHK07_002341"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO8102661.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARYGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35188..35886
                     /gene="ypfH"
                     /locus_tag="ACHK07_002342"
     CDS             35188..35886
                     /gene="ypfH"
                     /locus_tag="ACHK07_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8102662.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(35967..36167)
                     /gene="ypfN"
                     /locus_tag="ACHK07_002343"
     CDS             complement(35967..36167)
                     /gene="ypfN"
                     /locus_tag="ACHK07_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO8102663.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36195..37322)
                     /gene="dapE"
                     /locus_tag="ACHK07_002344"
     CDS             complement(36195..37322)
                     /gene="dapE"
                     /locus_tag="ACHK07_002344"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO8102664.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37326..37682)
                     /gene="yffB"
                     /locus_tag="ACHK07_002345"
     CDS             complement(37326..37682)
                     /gene="yffB"
                     /locus_tag="ACHK07_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO8102665.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9178 a         9796 c         9943 g         8830 t
ORIGIN      
        1 tacgtgataa atggtggggg cttccgctgt tcctgccttc tttaatcttg cccattttcg
       61 cccacattaa tactttcgcg catatttcct ccggtgaggt ttttctcttt tatctgcctt
      121 tggcactgat gatcagcatg atgatgtttt tcagctgggc ggcattgcca gggatcgcct
      181 tagggatttt tgtccgcaaa tatgcagagc ttggttttta cgaaacgctc tcattaacgg
      241 ctaattttat tatcattatc attctctgtt ggggcggtta cagggtcttt actccccggc
      301 gtaacaacgt ttcacatggt gatacccgtt taatttccca gcgtatattc tggcagattg
      361 tgtttcctgc aacgctgttt ctgatacttt tccagtttgc tgcatttgta ggattactgg
      421 cgagcagaga aaatctggtt ggcgtcatgc cttttaatct cgggacctta atcaattatc
      481 aggccttact ggtgggcaat ctgattggtg tcccgctgtg ttacttcatc attcgggtag
      541 tgcgaaatcc gttttattta cgtagttatt attcgcaatt aaaacagcag gttgatgtca
      601 aagtcaccaa aaaagagttc gcactctggc tactggcatt aggtgcttta ctgttgctgt
      661 tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac caattatacc ttgtcattat
      721 tgctgcccct gatgatgtgg ggagcgatgc gctatggtta taagctgatt tcactgctct
      781 gggcggtcgt gttgatgatc agcatccaca gctatcaaaa ttacattccc atttatcccg
      841 gctataccac gcagctgacc ataacctcct ccagttatct ggtattctct tttattgtca
      901 attatatggc tgtactggca acccgtcaga gaacggtagt cagacgcatt cagcggcttg
      961 cgtatgtgga cccggtggtt catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg
     1021 atgccccctg gtctgcgctt tgttatttac gcatccctgg catggaaatg ctggttaaaa
     1081 actatggcat catgctgcgg attcaataca agcaaaaact ttctcactgg ctgtcaccat
     1141 tgctggaacc gggtgaagat gtttatcagc tttcgggtaa cgatctcgcg ctgcgactga
     1201 atacagaatc gcaccaggag cgcattaccg cactggatag ccatctcaag caatttcgtt
     1261 tcttttggga tggcatgccg atgcaaccgc agattggcgt cagttactgc tatgtgcgct
     1321 cgccagtgaa tcatatctac ctgctgctgg gagagctaaa tacggtcgcc gaactttcca
     1381 tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc aatgtatttg caacgcgaat
     1441 tgaaagataa agtcgcgatg atgaatcgac tacagcaggc gctggaacac aaccattttt
     1501 tcctgatggc ccagccgatt accggtatgc gtggtgatgt ttaccatgaa attcttctgc
     1561 gcatgaaagg tgagaatgat gaactgatca gccccgatag cttcttgccg gtcgcgcacg
     1621 aatttggttt atcgtcgagt atcgacatgt gggtcattga gcatacgctg caatttatgg
     1681 ctgaaaacag agcgaagatg cccgctcacc gttttgctat taatctgtct ccaacctcgg
     1741 tatgtcaggc tcgttttcct gttgaagtca gtcagttgct ggctaaatat cagattgaag
     1801 cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc
     1861 aaatcacctt gcagcatctt caggaattag gctgccagat tgcgattgat gatttcggca
     1921 ccggctacgc cagctatgcg cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg
     1981 gcagttttat ccgcaatatt gtgtcaaata gtctggatta tcagatagtg gcatcgattt
     2041 gccacctggc gcgaatgaag aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga
     2101 tccgcgaggc agtgctctct ttggggatcg attatatgca gggttatctt attggtaagc
     2161 cgcaaccgtt aattgatacg ctgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa
     2221 tgcgggccga catttctcgt cggcccgcaa agcattaagc ggcgatttca ggtgtacttt
     2281 cttcttcaat tttcaaccgc cagccagcca cgccttccca gtattcttgc tccttttcca
     2341 gatcaagcag taccaacgca ttctgactaa accagtcatg cgggaaacgc agtgtccagt
     2401 gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt ggttgcctga cgttgattgt
     2461 tgagtaatac gccaaggcgc aatagctgaa tcagtggcag gaactgtttc ttcttaaaca
     2521 aggtaaagcg cggcagatcg tcgagtttaa tcgctttacg gtgatagcgc accagtgtcg
     2581 ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc gctgttttgt agaatataag
     2641 cggagtgacg atgcaaaccg ctgtggttga tattcaaccc gacctcatgc agcatggcgg
     2701 cccatcgcag tagcgcctcc agttgcggat gcgccagctt cggttgctgt tcccgccact
     2761 gttcgtacat ttgcatagtg gtatccagca cccgtcgggc ctgttcgctg tcgatgtgat
     2821 actggttggc gaggctgctg gcggtgcgac tacgcacatc ctgatgacgg aaacgtcctt
     2881 ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga aaggcgcagt tcacggatgg
     2941 ctaaagcatc aaacacaccg cataaaatcg ccagtcccgg aacgaagact gttttccgct
     3001 cttcggaaag acctggtaaa ctcagcgatg cgaaattacg gtgacgtaaa acttctttta
     3061 ccagtttttc cagacgttcc ggggtaatta tcccgtcttt ctcgcccatt tccattaata
     3121 cttcatgggc ggcttttatg gtaccggaag cgcccattgc cacgttccag ccctgaatac
     3181 ggaattgcca ggttaaagtt tccagttttt gtgctgccgc catgcgagca cgctgaaaat
     3241 tctctttatt gatgactccg ccagggaaat aaagctgggc aaagctgaca caccccatcc
     3301 ggcggctttc aacgagaatc ggttcgaaat tttcaccaat caccagttct gtagaaccgc
     3361 cgccaatatc aataaccagt ttgcgacctt tttccggttg ggtatgttcc acgcccataa
     3421 aaatcagacg ggcttcttca ttaccggaaa taatttcaat cgggtaggga atgacctttt
     3481 ccgcacgttt cagaaagtca gtggcgttca gcgcctgacg cagcgtatgg gtaccaacta
     3541 tacagacgct ggcaggagaa aacccttgta gccgttcggc aaacagcgac agacagttca
     3601 aaccgcgcgt cattgcctct tcactcaaca tattatctgg ccccaggccg tccgccagat
     3661 gcacccgctg tttcaggcgg ccaataatct gcatggcacc atctaccaca cgggctatga
     3721 ccatgtgaaa actgtttgaa ccaagatcga ctgcagcaaa ctcctgcgga cgaggggatt
     3781 tatcgtgtat tggcataggg ttattcaggt tgttcgagtg atttgatgta gtcataaatc
     3841 gccaactgcg cccgtacttt gcggcgattg ccgcggggaa cgtagcgatt actgagttct
     3901 ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc
     3961 cgctgcttca ggcgcggatc gagcagcggc gtcgccactt caatacgata atcaatattg
     4021 cgcgtcatcc agtcggcgga agaaaggtag acctttttat cgccgccatt ttcaaaaata
     4081 taaacccggt catgttcaag gtaacggtca acaatactga tggcacgaat gttgtcgcta
     4141 atgccttcca gattggggat cagcgaacac attccgcgaa ccagcagatt aaccggtacg
     4201 ccggagctgg aggccgcata cagacgatca accaggcctt tatcgacaag gttatttagc
     4261 ttcagggtga taccactggg cagcccttgc tgcgcgttgg cgatctcgcg gtccaccatt
     4321 tcatacaaca ggcggcggga gttttgcggc gacaccatta aataatcaaa tgtcaccgga
     4381 cggtatgggt tttcaataaa gttgaacacc cgccgtactt cgttagtgat gcgcgcatcg
     4441 gcggtcagca acgaatagtc agtataaaga cgcgcggttt tttcgttaaa gtttccggtc
     4501 ccaatgtgtg cgtaacgcac cacttcaccg ttttctttac gtgaaatcag gaacagtttg
     4561 gcgtgaattt tcagccccgg cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc
     4621 ttcgcccagt gaatgtttgc ttcttcgtcg aaacgcgcct gtaactcaac caccacggtg
     4681 actttcttac cgttgtgtgc ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc
     4741 acgcggtaaa tgttaatttt aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc
     4801 agttccagca catgctcaaa ggtgtgataa ggataataga gcaacacatc gcgttcgcga
     4861 atggcatcaa aaccattgcg gaactgggct ttatcaaacc aaatgtggcg taaacgcggc
     4921 agtggtttgt tcaccagatt ggctttgccg acattgggga aattaataaa gtctttaaaa
     4981 ttatgataac gaccgccggg gacgatggag tcgtagcggg aaatagtcag tttttcgcgt
     5041 aacacttcaa ccagcgcatt gggcatatcg cgctgataaa caaaacgcac cggctcagca
     5101 gttaaacgct gcttgagact ggaagacatc aactccatca ggctggcttc catctcatgc
     5161 actaaatcgt attcggcatc gcgggtcatc ttcattgaat aggcattcag cgcgtcataa
     5221 tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca gaatgttatc cagaagaatc
     5281 atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca caaagcgcgg cactttatct
     5341 gatgggatct ccagcagcgc gtaacggatg gtatcgccac ggataatttc caccgccaga
     5401 taggtgtaat catctttcag gaactgcact aagtcagtgt caggattgat taaaatcggc
     5461 gtaatgtgct gacgcagata ctgcttaaaa taatgacgca gccagttttg ttgattgacg
     5521 gagagctggc gttcattaat caggaagatc tggttgcgcg ccatctccag caatagctcg
     5581 ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc gggactgaat tttgcccagt
     5641 aaatggcggg aatgagagtt ggagccttgt tcttcgctaa taatgatgcg tcgcttcagt
     5701 tcagcgaagc ggactttata gaactcatca aggttattgg aatagatccc caggaaacgc
     5761 atcctttcaa tcagcgggtt agatttgtcc gccgcttcct gaagcacgcg ttcattgaac
     5821 gataaccaac tgagctcttt ttcgatgtat agcttttcct gacccattac cacttttact
     5881 ccgttttatt cacgggacac tgcttggata ttatggcgag caatattggc agatgtccaa
     5941 ctatgccaga aaagggggaa aaaaactgct ggagcttgcg atttacaggt gaattgaaaa
     6001 acgccagcgg cagagctggc gctttaacta cggggcatta ctcgtcggca gcgtagccct
     6061 gcggcggcag acgttgacca tccagccacg cggcgttttc gtgcattttc agacgaccat
     6121 cggcaaacca gctaatcacc agtggataaa tggcgtgttc ctgggtttgc acgcgggcgg
     6181 tgacgtcatc ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc
     6241 caccgtccag ttcatcggtg acgaaatgca ccgatgtacc gtgctcttca tcgccatttt
     6301 ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt
     6361 tcagcaaacg cccggcatag tgggagacaa acgccgggct gagaatgcgc ataaaaccag
     6421 ccagcacgac cacatcgggt gcgtacatgt cgatttcatg aatcaactcc cggtcatagg
     6481 cttcacgact gtcaaacgcg ctggcgatga gcgtatgcgt tgcaataccc gcctggcggg
     6541 cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa
     6601 ttttgttggt tttacaggcg tcaataattg cctgtaaatt acttccgttg ccggaaataa
     6661 gcaccacaat attcattatt cgataaccac gcgttgttcg gaatcagagg ctttgatgat
     6721 accgattttc cacgcgtttt caccgttggc attgagcagg gcgagggctt tgtccacttc
     6781 cggagcaggc agggcgataa tcatcccgac gccgcagttg aaggtgcgat acatttcatg
     6841 gcgctcaacg ttaccagccg tttgcagcca gttgaacact tccggccact gccaggagga
     6901 ttcatcaatc actgcctggg tattatctgg caatacgcgc ggaatgtttt cccagaagcc
     6961 gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt tcaatcaact ccagcactga
     7021 cttcacgtaa atgcgggtcg gtgccagcag atgatcggct aatggcttac catcaagttc
     7081 ggtggtttgc ggatcacaac cgctgacttc aagaattttg cgcaccagcg aataaccgtt
     7141 cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc
     7201 gtcgatgatt tctgattttt ctaccacgcc cacgcagaaa cccgcgacat cgtaatcctc
     7261 accgtgatac atccccggca tttctgccgt ttcgccaccc accagtgaac agcccgattg
     7321 cagacaacct tccgcaatgc cgctgatcac cgctgaagcg gtatcaacat ccagttttcc
     7381 ggttgcgtaa tagtcgagga aaaacagcgg ttctgcacct tgcaccacca ggtcattaac
     7441 gcacatggcg accagatcaa taccaatggt gtcgtgacgt tttaagtcca ttgccagacg
     7501 cagcttggta cctacgccgt cagtgccgga aaccagcacg ggttcacgat atttttgcgg
     7561 caatgcacac agcgcaccga agccgcccag accgcccatc acttccggac gacgcgtttt
     7621 cttcactacg cctttgattc ttccaaccag agcattaccc gcgtcaatat caacaccggc
     7681 atctttgtag ctaagagagg ttttatcggt cactgcttgg gtccccacgc gttacttgcg
     7741 gtagaaaaat aaaattcggc gcaattctaa cagggaaagc aaacgtttgc gagactgctt
     7801 tacacaacct ttttgcacgt cttttcccca ggcgcgcggc gaaagaagac ttgtgccagg
     7861 gcaaaggtta gttttcggat ggaataatct tctttcataa ccatttgaat ataaaataac
     7921 tttatctcaa accgttatca ttttgactaa agtcaacgaa aataatattg ccgccttgaa
     7981 gaaaggaggt ataatccgtc gatttttttt gtggctgccc ctcaaaggag aaagagtatg
     8041 aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc tgggactgat gcgtgagcaa
     8101 gatatcagca ccaagcgctt tcgcgaactc gcttccgaag tgggtagcct gctgacttac
     8161 gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg aaggctggaa cggcccggta
     8221 gaaatcgacc agatcaaagg taagaaaatt accgttgtgc caattctgcg tgcgggtctg
     8281 gggatgatgg acggtgtgct ggaaaacgtt ccgagcgcgc gcatcagcgt tgtcggtatg
     8341 taccgtaatg aagaaacgct ggagccggta ccgtacttcc agaaactggt ttctaacatc
     8401 gatgagcgta tggcgctgat cgttgaccca atgctggcaa ccggtggttc cgttatcgcg
     8461 accatcgacc tgctgaaaaa agcaggctgc agcagcatca aagttctggt actggtagct
     8521 gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg acgtcgaact gtataccgca
     8581 tcgattgacc agggactgaa cgagcacgga tacattattc cgggcctcgg cgatgccggt
     8641 gataaaatct ttggtacgaa ataaagaata aaaataatta aagccgactt taagagtcgg
     8701 cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta tgacgcgccg
     8761 tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta gtttgcaaca
     8821 tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata ttaacccggc
     8881 gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct gtaaagggaa
     8941 aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt tactgttgcc
     9001 gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt
     9061 ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc cacctgcggc
     9121 aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg ccggtatggc
     9181 gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca tctctattac
     9241 caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg caattatccc
     9301 gattttaatt ggcgtgctgg tggggtacgc gctctctttc gcaatgggaa ttgtcgatac
     9361 cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc cgcgcttcga
     9421 gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg aacacgtagg
     9481 gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca
     9541 ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc ggcttctttg gctctacgcc
     9601 aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt acagtacctg
     9661 ggttatcggc ggggcggcga ttttcgctat cctgctttcc tgcgtcggta aactggctgc
     9721 tgctatccag atgatcccat tgccggtgat gggtggtgtt tcgctgctgc tttacggtgt
     9781 catcggtgct tccggtattc gtgtgttgat cgaatcgaaa gtggattaca acaaagcaca
     9841 gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga aggtaaacat
     9901 tggcgcggcg gaactgaaag gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt
     9961 gattttcaaa ctgattagtg tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc
    10021 ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat
    10081 gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca
    10141 caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt gcaagtttct
    10201 ggccggggga taactcctct ttactggccg cgctgcaaaa cgtgctgcgt caggaacata
    10261 gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg
    10321 cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg gataaacgca
    10381 cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc tgtatcgaca
    10441 acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat ctctacaatc
    10501 gaattctgga atcgggcaaa acacggttgt tgatcaccgg cgatcgtcca ccgcggcagt
    10561 tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc
    10621 agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt
    10681 ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca
    10741 cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga
    10801 ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg tcggatgcgg
    10861 cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg ccggataaga
    10921 cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca tcttaaccca
    10981 cgatttccag tacctgctcc ggtggacgac caattcgcgc tttgccattc gcgaccacaa
    11041 tcgggcgctc catcagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa
    11101 gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac
    11161 gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca tcggcgggtg
    11221 tctcaaggta gagcaccact tctggctcca cgccgttttc tttcagcagg ttcagcgttt
    11281 cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc
    11341 ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg
    11401 gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc
    11461 gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg
    11521 cgcagccagc tcctgatcgc ggttatttag cgcggcttcc gcctgtgcca gcaaatccca
    11581 gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg
    11641 tggttgaccg ccttgcagat aagcgttcgc caggttgagc tgcaacacag gattggtgcg
    11701 taaatcgcgg gcatttttca gacgattgat cgcctcattg gctttgtttt gcccaagatc
    11761 gatatcagta gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg
    11821 cagcgtttta cgcgcctcgt cgtatttatt ggcttccatt gcctgtaaag cacgaccata
    11881 ttgtgctgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt
    11941 gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc
    12001 ttccgacgac tgcaccacca tcgggcgcat ctgattagca cggttgcggg catctgccag
    12061 acgactttcc ggcaacgggt gagtcaataa aatttccggc ggacgcgagg agtaacgcgc
    12121 ctgatcgagt aatttttcga ggaaggtcgg catcgcctgc ggatcgaatc ctgagcgttg
    12181 cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga aactgatcat
    12241 cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct gcggactggc
    12301 catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgctg
    12361 atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac
    12421 tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca gcaccacgtt
    12481 gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaaa aatgaaacgg
    12541 tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa tatattgcgt
    12601 taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc
    12661 catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca tatccggcaa
    12721 ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca atggtcatag cagcaatgag
    12781 ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt atttctgaag
    12841 atagtcattt gaccgatact gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg
    12901 gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag
    12961 tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga
    13021 aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag
    13081 cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc
    13141 tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg ccaaccgtgc
    13201 gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggtttttg
    13261 tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcaa cagggcatct
    13321 acttaatccg cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc
    13381 cgcgccgcta tccggcgtta atggatgcgg gcattattga tgcaatggcc gaaaatatgc
    13441 gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg
    13501 gtttgctgac catagccgtc tatctggtgc tggtgccatt gatggtcttc ttcctgctga
    13561 aagacaaaga gcagatgctg aacgccgttc gtcgggtgct gccgcgtaac cgtgggctgg
    13621 caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc
    13681 tggagatgat cgtggtgggg atcgccacct ggctggggtt cttgctcttc gggctgaact
    13741 attcgctgct gctggcagtg ctggtcggct tctcggttct tattccgtac attggcgcat
    13801 ttgtggtgac cattccggtg ataggcgtgg cgctattcca gtttggtgct ggcacggaat
    13861 tctggagctg cttcgcggtg tatctgatta ttcaggcgct tgacggcaac ctgttagtac
    13921 cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta tcggtggtga
    13981 tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccattg gcgacgctga
    14041 tcaaagccgt gatccacgcc tggcccgatg ggcaaatagc gcaagaataa tcttcagccc
    14101 gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa tagcccgata
    14161 acacatactc accagcactg caccgccgat aatattaccc agcatcaccg ggagcagatt
    14221 ggcggtaata aaatggctta ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag
    14281 ttgccagaag gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa tcacaaacaa
    14341 attggcgata cagtgctcaa agccactggc gacaaacagg gtgatgggca aaatcatggc
    14401 gacgattttg tcgcataacg aacgcccgca ataactcatc cacagcgcca ggcaaaccat
    14461 cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca ttttgccctc
    14521 ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca gcccggaaaa
    14581 ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca ccagaagtgc
    14641 gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatca ccgtcattac
    14701 cgacgaggtg aacaggctgg tgccgcaaac cgccagcaaa ataaagccga gtgtaaagca
    14761 aaggccgccc accagatggg ttaatgcctg tgaagacggg gcatcggcga taacggaaag
    14821 gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta aaaagaccga
    14881 cactttactg cgggcaattt tatgggcggc aatccgttca gcgattgccg cttttctggc
    14941 gctcaactgc aagtcgaaag agagtttgtt tctcatgcat actcctgcaa aagcagatta
    15001 caacacctcg cgaaccgaga tccccagacg ctgcattcgt gacagcagcg tggtgcgctt
    15061 catccctaat cgtgtcgccg cgccacgggg cccggcaaca atgccattgg tttctcgcaa
    15121 tacctgaaca atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa
    15181 ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta aacggctttg
    15241 tcggacatta agatgtaaat tcagactgtt accacgagtc aacagtaccg cccgctcaat
    15301 cacgttttcc agctcgcgca cgttgcccgg ccaatcccac gacatcaact ggcgtagcgc
    15361 ctcggtcggg atggcgtcaa ttgcgcgatt catatggcgc gccatttttt gcgtgaaatg
    15421 ttttgctaaa agagggatat cttccggacg gtcgcgtagc ggcggcaatt ccagtgggaa
    15481 gacattcagg cgataaaaga gatcgctgcg aaactggcga tcttcaacca tttgccacaa
    15541 atcacggttg gtggcggcaa tgactctgac attcaccggg atcgttctac tcccgccgag
    15601 acgctcaatc tcccgctcct gcaatacgcg cagcagttta ggctgaagtt ctaacggcag
    15661 atcgccaatt tcatcgagaa acaacgtgcc gccatcggca atttcaaaac gaccacgatg
    15721 ggtattaatc gcaccagtaa acgccccttt gtcatgaccg aataactcac tttccagaag
    15781 actggcgggg atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag
    15841 ttgatggatc gctctggcga ttacctcttt gccagttccg gtttcaccgc aaatcaacac
    15901 cgtactgtcg ctcttcgcca caatatctac ctgctggagc aggtcttcca ttgcctggct
    15961 ttgatagata atgtcaccga cgcccagatt cgaaaggagc tgctcgctaa gctggtggtt
    16021 ttcttgctgc aaactttcct gcaaatcggt catgctacgc caggcatcgg cattgccaac
    16081 ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg cagttttcct cactaaagag
    16141 agtggaagag gtatgcgcca gcaacaatgc acccggtgta tggttgccaa aggtaagcgg
    16201 tataaggagc gcagattcgc agccgttaga tgcaagtaag tgtaataacg ggtcgcgttg
    16261 ccagagaaac agatcatctg cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg
    16321 tatcagcaat acactgtcgc caggcatatt gcgtggcaga cacgcacaat gtgaggcaga
    16381 aagatcgctg caccacaggc tgaacttctc gttctttcga tgatcgccca gtaccatact
    16441 gactgaagcc agaccgaaaa aatgatggat ctcacgggcg acgtcagcga tcagatcgtc
    16501 gagatcaaga tgtgacagca ctgcattagt aatatcaacc agtacccgat gatgatcgca
    16561 atctttcttc agcaaacgca gcgccacctg ctgttcttca tcattgagcc atgattgcag
    16621 aaccggggtg ataatgcgtg ccagccagtt aaacagtgtg caatcctggg gagtaaaacg
    16681 actgccgtgg gtgcgaataa agcgtagctc cgccattcca ctactgccga gaaggacggc
    16741 gtgacgcgaa taatcgacca tctcgccctg atactctact gttgccgtca catcgctgcc
    16801 atcatcatgg tagcggtaat agaagttgct tttgagaaca tgcagttcaa taagttgtac
    16861 cgggctgaac ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct tgccgtgttt
    16921 ctgcgctaac cgctccgcga gcattccgtt taccgcttca attgttattc cttgcggcgg
    16981 ggcaaacatc gcctcgtctg acatagccat caacagtcct tattaatgag aaatccttct
    17041 cacgcaattt ttctggccat cagatagatg gccggttcct gcacaatttc atccaacaga
    17101 tgaatgaatt gaatgctacg cgcctgccac acagacgtcg cgtcatccgc cagtggcgcg
    17161 aatgcccggg tcaacagggc ggtgtggctg gtgtcaataa caatctcacc aaaagtcatc
    17221 acccccagca tcttacgtcg ggcttgctcc tcttcgacca gtgcaagcca atcttcgtat
    17281 tccgtcagtg ggcagcagaa ggcgacattc agacaatcaa tcacgccaac gtgatgaccg
    17341 atagccagtg agtaatacat tacctgagag ctgtgttccg gcatctcgtc gctactggcg
    17401 acaaactttt ttcgcagcgt ccagaaaaca atttccccgc actcctcagc catgtaaccc
    17461 ccggagaacg cgaaagagtt gctgaacgat actattgaga cgcggatcgt cggcatcgcg
    17521 caaccaggtg ttaacccgat ttcctgtagg atcatcgctt aaatgacgca ggagccgatc
    17581 gcaaatttct cgcccctgac gatagcccgt cagccgccgc gcttctcgct caatggcgat
    17641 acgctgtgat ggcgggatct gcggccacaa cggttgcata gtcaccccgg tgggatcgcg
    17701 ataatccaca gcgtgaatct tctgttgcag caaaccgagc gccacggcga aaccgtgaat
    17761 ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca tcaatgggga caatggtgtc
    17821 gctgccgccc cagacgctgt agagatcgtg gaaaataccg ccgccgacac cgcacgcgcc
    17881 gtaggaaaca caaattttat tatcgggggc agactcatac gcccgaagtg caggcatacg
    17941 catcgcccgg gtgactgcgc cagtaaataa caaaatatcg gcgtgacgcg gtgatgaaac
    18001 aaccttaatg ccaaaacgtt ctgcgtcgaa tacgggtgtg atggcagcaa aaatttcgat
    18061 ttcacaggcg ttgcagccgc cgcaatcgac gcgataaacg taagccgagc gacggatatc
    18121 ttgtaacaaa tgctgcttca tcttttgcgt ttgctcgtcc agcgtgatgg gctggctgac
    18181 atgttgcgta agcactggac tcatagctgc tccttagcca ccagcggtac atctgtatcg
    18241 tcgttgatca gcgtcgcgcg ttgtttgcac tccgggcaga cgctcgcttg cgcccgcaac
    18301 atttcgcggt tttgtggtgc attttgttgc tgtgccaaca attcagcagc cagtgcgacg
    18361 gttttttgcg gggcaaacgg gcgttcgcaa cggctgcaac gttgtagatg gaacgtcgcg
    18421 cgggtataga gatcggcttt attggtgacg gtcagttcaa agttattggt aagctggatg
    18481 gctctggtcg ggcacacttc ttcacaacgt ccgcagtaaa tacaacgccc cagatagagc
    18541 tgccaggtgc gcgaattttg ctggtcgtcg gtctggatag tcagcgcatt tgccggacaa
    18601 gcacaggcgc aggcaccgca ggcaatacat tggctgggca tcaggtccgg tttcccgcga
    18661 aagcctgggc tgacctccag tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc
    18721 ataatagttt tcagtaactt cagcatcttc tgtccttact tcagcggcga acggttacga
    18781 tcaatgccgt agcgttcgat ctctttatac ggcacggttt ttgactggcg cttgcgcaca
    18841 tcaaccagcg tcacacggtc ggtacaggag tagcagggat caaggctacc gataatcagc
    18901 ggtgcgtcag aaacggtatt gccgcgtagc atgtaacgca gcaccggcca gttggcgtag
    18961 gtggcggcac gacagcgcca gcggaacaat ttttggttat caccgagcat gctccagtgc
    19021 acgtcttcac cgcgtggcgc ttcaacaaag ccgagcgcga atgcgtgagg tttatagcta
    19081 aagccttcgg tcagcagcgg ggtatccggc atgttgtcga gggcaaattc cagcattgcc
    19141 agcgaatcaa acgtctcttt gacacggacc atcacgcggg agaaaacatc gccgccggtg
    19201 aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa
    19261 tcacgggcaa aaccactgcc gcggatcagc ggccctacag ggctataatc acgggcgatt
    19321 tgtcggtcga gaatgccaat gccctgagtg cgttgttcca tattcggcgt agcaagcagc
    19381 atctctacca gctccgacac gtcggcgcgc atctcgcgca ccagtttcag cgtttgcaga
    19441 cgttgctctt tgagaatatc gcggcgaaca ccaccaatca gattcagacc gtaggtttta
    19501 cgcgacccgg tcagcaattc cgccattgtc atcgactttt cccgcacgcg aaaaaattgc
    19561 ataaagccgg tatcaaaacc aacaaaatgg caggagaggc caaggttgag caaatgactg
    19621 tgtagccgtt cgacttccag cagaatcgag cgaatggtat gcgctcgttg cggcacctca
    19681 atcctcagtg cattttcaac cgagttggta taggccacac tgtgggcaaa accgcaaatc
    19741 ccacacacgc ggtccgataa gaaggtcact tcgttgtagc ccatccgcgt ttctgccagt
    19801 ttctccatgc cgcgatggac ataaaacaga cggtaatcag catcgacaat ttgctcgcca
    19861 tccacaaaca agcggaagtg acccggttca tcggaggtga tatgcagcgg gccaataggg
    19921 atcacccgcg cgtcgctgtc gccctcgttg ataaacggat acgtttcggt atcagtcgtc
    19981 ggttcagggc gcagtcgata atccatcgcg tctttgcgca gcggatgcat atcttccggc
    20041 cagtcatcgg gcaacaccag gcgacgctga tccggcaggc caaccggaat cagcccgtac
    20101 atatcgcgaa tttctcgctc gccccagacc gcggcaggga cgcgcggtgt gactgacgga
    20161 aactcccgac tgtcggcatc gaccagtgcc ttcaccacga tccagcattt ttcggcccct
    20221 tccatagaaa gggcataata aacggcgtaa tgaccgttaa gtgtccgctc gtcgttgcca
    20281 aacaggaccg gaagccagcc atcatgttga taataaagat actgtacaac gtcaggcagc
    20341 agattgattt tcaccgtaat ggtgacctgt tcaagcgttt gtcgctcttc atccagcacc
    20401 gcgccgggga actgcgtttt cagggcggcg agaatcgctt cgccacgatt tgacgatgaa
    20461 ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct gaaggcaaaa aatcatgcca
    20521 gatgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg actatagtgg aagcgcctgc
    20581 caggatcctg atgacaggtt gtggaatatg cgttcccatc gccagcatca tgaccagcag
    20641 aatcaccatt ggcgaggtgg tcaaccagcc aagttcaccc cggttaacgg cctgcggcgg
    20701 tttcgccatt aacacccgcg cagccatccg taccaggccc gccagcacca gcgttaacag
    20761 caataacagc aggacgataa tcagcagatg attacgtgcc agtccggcgg taacggtcat
    20821 aaattcgcta agaaaaatgt tgaagggcgg catccctgcc agcgccagcg caccgccgcc
    20881 aaacagcacg gcggtaaatg gcatgatttt gagcatccca cagacgacgt tgagatcgcg
    20941 cgtgccgtac ttgagcagta cattgccgga accgcagaac agcagcgttt ttgccagact
    21001 gtggtttaag gtgtgcagca gcgcggcaaa aattcccagc ggcccgccaa tgcctagcgc
    21061 caccgcgacc agtcccatgt tctccacgct ggagtacgcc agcagacgct taatgtcccg
    21121 ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac atgccgaaga tgagcaacaa
    21181 ccggttgggg aaatcgctgc cgatggcctg gcaaataatg atgtaatagc gaatcagcac
    21241 caacagcgcg cagttcagca atacggcgga gagtagggcg ctgaccggac tcggcgcttc
    21301 actgtgagca tccggcagcc aggcgtgcat gggaaatagc ccggttttgg taccaaagcc
    21361 aattagcaca aacacaaagg ccaacagcat taatgttgga tcaagcaagg acgattgctt
    21421 aagaacctcg ctccagaata tcgccatttc tgcctgcggc ataacgctgg cggcgttggc
    21481 gtataccagc acggtaccga acagaccaaa agcgacacca acagtacaaa taatgatgta
    21541 cttccatgca gcttccagcg atgaacgctg accgtaaatg cctaccagaa acgccgagct
    21601 taaggtggtg gcttcgatcg ccgcccacat cacaatcagg ttattgctgg taacaaccag
    21661 cagcatggtg aacaaaaaca gatggaagaa accgtagtaa tcgcacagcg taacgggtga
    21721 aagctcgccg tgctccactt catgacgcat gtagccaatc gagtaaacac cggtaagaaa
    21781 gccaatcaca ccaagaatgg cgaggaacaa accgcccaga ccatcaatat gaagccacag
    21841 tcccgcagcg aatatttctc ccgcatcagc ggcggtttgg accacccaga gtgccagaat
    21901 cagcagtagt gtgatcccta agctatgtaa tacggtcacc gcgcgagtcg cagagtgccc
    21961 ccgtttccgg caggcaaaac agagcagcga aaaaagcagc ggcgtgagca ggagtaaagc
    22021 gaacatcaca gaataactca tctcattatc ccttcagcgc ggtcaagttg ttcacgtcca
    22081 gcgtgccgtg ggtacgccat atttttcttg ccagtaacac catcacaatg acggcgaaga
    22141 tggcgtcggt agcgatacct atttccacca gttccggtgc acgccaggca agaagcgcca
    22201 gcaccagatg ggagccgttt tccatcaggc agtaaccaaa aatttgccgc aggatattgc
    22261 gctggctgac aatgcacagc aggccaagca gaaaatgacc taacgctacc gccagcgccg
    22321 gtttcagccc ggtagccatc ggtagcttca cgggctgaac gacaaatgcg caaagcagga
    22381 caattaacgc cgcgagcagt gccatcattg ccggaccgaa taacgctttt tccgggatgt
    22441 tctggggaat atttcgtgca gcgtaagtca tgattaacgg taccagcagc actttggtga
    22501 taaaggcgct ggcggaccag atcagcagtt gctctgcggc gaacaggcac gagagagtgg
    22561 cgaaaataga caccagcacc agcgactggc aggcgtaaaa tccgcatgac aggcgatagc
    22621 ttttgacgct aatcacaaac agcgatgtca gcatcatcag tcccgccaga ttatttacga
    22681 tcatagaacc ggtcattcat catctcctta ctgcaaccag gtcgcggcga ttacgctgga
    22741 aatcagcgac atcacaatca gtacaatcaa caccaggcgc attgatcctg gcagcggtgc
    22801 ggcatcggcg acggcctcgc tcggtttgcc aggcacgacg cgaccaaacc agcgaataaa
    22861 ccaagcgaaa ctggcgaccg attcaatcat cagcagaatc atggcgggca gcaggatcca
    22921 gtactccact gacaacgcaa aaccggcggc aaacagcggg aatttactga agaagccgtt
    22981 gaacggcggc acgccggtaa tcgccagcgc tgccacgcag aaacccacgc ctggcaacgg
    23041 cagggtgtgc aatacgccac gcagacgcgg caacaagcgc gtgccgcagc tgtaactcag
    23101 cgcacctgct acaaggaaaa acaggctttt agcgaacgcg tggttgacga tgtaggcgat
    23161 gctaccctcc agcgccagcc gcgagccgaa gatggagagc gacaagccga agaacatcca
    23221 gccaagttga gtgatggtcg accaggccag caaccgcttc atatcctgct gtggcaaata
    23281 catcagaaat ccataaagaa tggtgaccag ggccatgacc atgccaacac cgccaatcac
    23341 atgcgggata ttgccgccgt cgataatggc gcgggcaaaa atgtaaacgc ccactttcac
    23401 catcgatgcg gcgtggagat aggcgctgat cggtgttggc gcttccattg cgtccggtag
    23461 ccacgcttgc atcggtagct gggccgattt cccccacgcg gcaaacagga tgccgccata
    23521 aaccagataa cgtgcgtcgc cgtgtaactc gctcatcgcg ctaagcgcaa acgttccggt
    23581 ctgcaaaaac agcgtggcgg cggcaagata caaccccaac gagccgatat gggtgataag
    23641 caacgctttt agtgctgaac gctgcgcttt atcgctctgg taataactga tcaacgccca
    23701 ggagcagccg cccgtaattt caaaaaacaa caactgaccg agcagcgtcg aggagagtac
    23761 cagtcccgcc atcgcgccga taaacaacag caaaaatgcg taataacgat tcgtgccgtt
    23821 atgcgggtgt tcgcgatttt tatccgtcag ataacccgtg gagtagatcg tgaccagcaa
    23881 accgagaaac accaccacaa acagaatcag cgtactcacg cgatcaatga ccaggccaaa
    23941 caatgccaca tcccccacgt tgaccaacgt caacgtgacg gcaactttat cggcctgata
    24001 aaacgcggag atcagtgaca acatgcacag cgtggtcagc gcggcgaaca aaacccccca
    24061 ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa aaggcagcaa
    24121 taacgtcgtc agagcaagat tttccatatt ccgtcagtgc tccttacaga ccggttaacc
    24181 agaagaccca tgcaagcgca gcaaggctga agccaagcca ggtcacatgg tgaatgagta
    24241 aaaaacgccc gcgtgccagc gtgttttctg cgatagaggc cagaacaaaa atcagcaaaa
    24301 ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc gagagaaagc tcttgcgcgc
    24361 gcccaaaggg cagaaacagg gccacaaaca gtgctgccat cacgacctgt ttcagcccca
    24421 gcccccattt cgctagcgcc agcccggcac cggaatattc ggtcagcggg ccttcctgta
    24481 attcctgttc tgcttcagca acatcaaagg gaatttttcc catctcaatg aagcaggcaa
    24541 aaccacaggc cagtaacgcc agtacggtgg ttagcggcga gttccagccc gttgccagcg
    24601 tattgctgat catctcgata tgcgtggaac ctgctatcag cgccaatacc agcagtgaga
    24661 gaatgagcat gggttcgacc agaatgccga gcgtcaactc gcgactggca ccgactcccg
    24721 caaacgggct tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac agggcaagaa
    24781 gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta ataaatagtg
    24841 gtagggccat cgccagcacc agcatgctgc tgattaatac ccacggcatc aggcggaaca
    24901 tcagacctga agatgtcggc gcaacttcct ggcgtttaaa cagtttgtgg atatcgcgat
    24961 aatcctgcca gatccccggt ccgcggcggg agtgcattcg cgcgcgtatc tgccgggaaa
    25021 tacccgtaaa aagtggggtt agcatcagca gaatcacggc ctgtgctaac gcaaaaatga
    25081 ccagatatcc gtcgcaaaga gtttgtctca tggtgatttc cttagacggc aatagcgatt
    25141 agcagcacaa ccagtgcggc gaccagatac aggcaataga gacgaaagtc gccgctttgc
    25201 agatgctgta tttctttggc cagccgttgg gtggcgctca cgatggggcg gatcacccgc
    25261 tcatcccaga agggttctgt gctctgagcc ctggcggtga cgtgcgcaag acctttattt
    25321 agcctcagcg tagggtcgag ctgttgtcgt agacgaaata gcgcagaaaa gaccacacgc
    25381 agcggctgca tcacgccatt gcctgacggg gccatcgcat tttcccagcc gtagccgcat
    25441 gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca cagccagtaa
    25501 aggccaggca gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg ggtgacctgt
    25561 gtatgaaacg tgccgggtac aagtgctatt ccgctggcga cagtgacggg aggggtattg
    25621 gtaaacgcat gggcaatatg cattatcttc ggtgccagcc aactggcact aagcgcaatt
    25681 aatacgcaga gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg cacttcctgt
    25741 gcctcttcag cgtgtgtact gcgcggcgca ccacagaaag taataccgta catttttacg
    25801 aagcacatta ctgccagccc accagtgact gccagcatta ccatagcaat aggacccgca
    25861 agttgtagcg ctacggcttc cacacgactt agtgagaaca gcgactgcca ggtgtaccat
    25921 tcgctgataa aaccattcag cggaggaatg gctgatatcg cgaggcaacc aatcaggcat
    25981 gctgcggctg tccacggcat ccgtttcgcc agtgccccca ttttttccat gtcgtgggta
    26041 tgcaaacgcg aaataatcgc gcccgcgccg agaaatagta gccctttgaa cagcgcatgg
    26101 ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg gtcgtgcagt
    26161 gacagaccga ccatcgccac accgactgcc agcaaaataa tgccgacgtt ttcgacggta
    26221 ctccaggcca gcagccgttt gatatcctgt tccgccagcg catatagcac gcccaggagc
    26281 gcggagattg tgccgatcgc catcaccaga atgccccacc acagcggcaa acccgtttgc
    26341 gccagcagat ccatcgccac tttcaggatg ccgaaaatac cgattttgac cattacgcca
    26401 gacatcaacg ccgaagcgtg cgatggtgct gcagggtgag cgcgcggcaa ccagctgtgc
    26461 aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa caccgccgac
    26521 gccagccccg gagaaagtga aagcgtgcgg aaactggcaa aatcgaggct gccgctttcg
    26581 cgtcccatca gcaagaaggc gatcataatc agcaccgatc cggcgtgggc gataaaaaag
    26641 tagagcatgc cagcgttgat cgacgtttta tcctgcctgg caatgaccag aaaccaggaa
    26701 gacagtgaca tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat caccagcagg
    26761 gcaaccatcg atgcgatgaa aatattcata aagaagccca tcgccgccgc gcctttgccc
    26821 tcgtattcgc gcatataagt caatgaatag agcgaacaaa ccaccaccag caaggagata
    26881 accagcacca taaatgccga gaggctatcc attcgcaggg ttaaatcggc aaagtcaaaa
    26941 ggggtggcaa aacgggcgac taaagttacg cctgcatgta attgcgtaat tccgctgata
    27001 atgccaatca cgccgcccac cagcgatgtg atgccggaaa gcttaatagc cagtctgtcc
    27061 agaccgagta aaaacagcga agccagactg gcaaacagat agaggatcag cgaccaggtt
    27121 aataattgca gggcatccat tagcgttgct cctgagtgag ggaaagggga tcctccccgc
    27181 cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt catctgacgt tgcaggctat
    27241 cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg aacgcaggcc ggaccttgtg
    27301 gcaagaaatc acacaggtcg catttcaccg cgacggtctg gacacccggt tcccagcgca
    27361 gcaaagcatg ttgggttggc gcgttttctt cgccgtcttt gagtgagcct tcagcctgaa
    27421 aaacatattg cgcaggggca ttcaccggac ggcttcctga agcgctgatt gcgccaaatg
    27481 ggcagaccac ggcgcaaagc ttgcagccaa tacagaggtt ttcgttgagt tggatcgcat
    27541 catccctctg ggagatggca ttgaccgggc agacctgcag gcacggggct tcctcacagt
    27601 gatgacacac gacaggggca gtgagtgttg acgtcttcgc cagggccagg cgcgggtgtt
    27661 gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc gagacaggta ttacatcctg
    27721 tacaccacag tggttcggcc accacaaagc ggttcatgtc ggctccttga aaatgatatg
    27781 aaacattgca tttcatattg cataaaggat gccagtctga ttaatttaat atattcaata
    27841 agttatgaat taactggatt ctgaacggta caaaaatgat ggtcattaac gttcagcaaa
    27901 aaatggattc gacacccgtg tcgcagggga acgcgagaat gtgatcttga tcacgagaag
    27961 tgaggtatgc tgaccgcaag cgcagccagc acggaatgga gcaaagtaat caggcgtgtt
    28021 ctttcaacca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg tcaaagacat
    28081 gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatacca tcgtaggttt
    28141 tgcccatgaa ggacttttcc ccccagacgt cgaattgctc gcacacctgg tggtcctcat
    28201 cagacaggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt ttttcgggtt
    28261 tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc atgttatcgc
    28321 gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag aaataaacca
    28381 gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga tccggcaagc
    28441 taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc catcctgttc
    28501 atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt ctgtacatag
    28561 ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt gggcagtaat
    28621 ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt tgcgtgcgcg acaccagctc
    28681 cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga
    28741 gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg gacgcgccgt
    28801 cgtgcgcttc atcacgatta aaagatccag ttcggcacct ttcaacggta acgttgattc
    28861 aatcagagta atggcattcc atgaaccgga aagcagcatg ataaacgtga actcttctcc
    28921 cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat gacgggtgat
    28981 ggtgttcaca attccagggc gatcggcacc caacgcagtg atcaccagat aatgttgcga
    29041 tgacagtgtc aaactggtta ttcctttaag gggtgagttg ttcttaagga aagcataaaa
    29101 aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc cataccaaac
    29161 gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa gtattgtcgc
    29221 gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat
    29281 tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca ctggcgagtc
    29341 cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg agctggctga
    29401 cgggcgcatt ccggtgattg ccgggaccgg tgctaacgct actgcggaag ccattagcct
    29461 gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc cttactacaa
    29521 tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgagc atactgacct
    29581 gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc cggaaacggt
    29641 gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag ggaacttaac
    29701 gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga gcggcgatga
    29761 tgcgagcgcg ctggacttca tgcaattagg cggtcatggg gttatttccg ttacggctaa
    29821 cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc attttgccga
    29881 ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg tcgaacccaa
    29941 tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg atacgctgcg
    30001 cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca
    30061 tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcaa aagtcgcgcc
    30121 tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt tctgactcac
    30181 gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg cttgcggagc
    30241 ttcatgcccc ggctggaatg attttgccgg tgacctccgg tgattatgca atcccggtga
    30301 ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc cagccgctgg
    30361 cactagtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg ctggtggaaa
    30421 atggtcgtgg caatactctg tggccgcagg tggttagcgt gctgcaggcg aaaaactaca
    30481 ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta caatggaacc
    30541 gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt
    30601 atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa ccggttgcag
    30661 acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc gccggtctgg
    30721 ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc actatggacg
    30781 tacaaagtgc agctgatgac accggtttac caatgctggt cgtacgcggg ccgttcaatg
    30841 tggtttggca acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg accgacagca
    30901 cccgttcgca gggcaacatg gccgtaactt ataagccgct gtctgacagc gactggcagg
    30961 aactgggcgc gagcgatcca ggcctggcat ccggtgacta taaactgcag gttggcgatt
    31021 tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg actcagagtc
    31081 agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa tacagggctg
    31141 gaatcatccg gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacgc
    31201 gattttagcg gtaattcgca cgaaatttgt ttgtcggacg tagttcggat aaggcgttca
    31261 cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa acgttaattc
    31321 acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta aagcgaaaac
    31381 cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc
    31441 aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca acaagttcaa
    31501 ctacttcatt atgagcaaac tggctgaagc gggtatcccg actcaaatgg agcgtctgct
    31561 ctccgatacc gaatgtctgg tgaaaaagct ggatatggta ccggttgagt gtgtcgtgcg
    31621 taaccgtgct gcgggctctc tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa
    31681 cccgccgctg ttcgatctgt ttctgaaaaa cgacgccatg cacgatccga tggtcaacga
    31741 atcttactgc gaaacctttg gctgggtaag caaagagaac ctggcgcgta tgaaagagct
    31801 gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc tgattctggt
    31861 cgacttcaag ctggaatttg gtctgtacaa aggcgaagtg gtactgggtg atgagttctc
    31921 cccggacggt agccgcctgt gggacaaaga aacgctggag aaaatggaca aagaccgttt
    31981 ccgccagagc ctcggtggcc tgatcgaggc ctatgaagcc gtcgcccgcc gcctgggtgt
    32041 acagctggac tgatttttct gttcatcatc ttgccgtgct gctggcacgg caagacaacc
    32101 attctcgtaa gatgtgcatt gaacgctcat tcctccgcca tttcatcccg ctttcctgtg
    32161 gtaatcttcc tctgaaccac ctacgatcat ttctataatg aatatattgt tgaggtatct
    32221 atgcgttggc aagggcgacg tgaaagtgac aatgttgaag acaggcgcaa cagctctggt
    32281 ggtccgtcta tgggcggtcc cggttttcgc ctgccgagcg gtaaaggcgg gctgatttta
    32341 ctgatagtcg tgctggttgc aggctactat ggcgttgatt taaccgggtt gatgaccgga
    32401 cagccggttt cccaacagca atcaacgcgg tcaattagcc caaatgaaga cgaagccgca
    32461 aaattcacct cggtgattct ggcaactacg gaagacacct ggggacaaca gttcgagaag
    32521 atgggtaaga cctatcagca accgaagctg gtcatgtacc gtggaatgac gcgtaccgga
    32581 tgcggggcgg gccagtccat aatggggccg ttctattgcc cggcggatgg cacggtttat
    32641 atcgatctct ccttctatga tgacatgaaa gacaaacttg gcgcggatgg cgattttgcc
    32701 caggggtacg ttatcgccca tgaagtcggt catcatgtgc agaaactgtt aggcatcgag
    32761 ccgaaagttc gtcaactgca acaaaacgcg acgcaggcgg aagtgaaccg cttatctgtg
    32821 cgtatggaac tccaggccga ctgttttgcc ggtgtctggg ggcatagtat gcagcagcaa
    32881 ggcgttctgg aaaccggcga tctggaagag gcgctgaacg cggcgcaggc catcggcgat
    32941 gaccgtttac aacagcaaag tcaggggcga gtagtaccag acagtttcac tcatggcact
    33001 tctcagcaac gctacagctg gtttaaacgt ggtttcgaca gcggcgatcc ggcacaatgc
    33061 aatacttttg gtaaaagcat ttaacttccg gggcagggat ggctgaactg actgcgcttc
    33121 acacattaac agcgcaaatg aaacgtgaag ggatccgccg cttgctggtg ttgagcgggg
    33181 aagagggttg gtgttttgat catgcgctta agttacgtga tgccttacct ggcgactggc
    33241 tgtggatttc gccgcagcca gatgctgaaa accactgttc tccctcggca ctacaaactt
    33301 tacttgggcg cgagttccgg catgcggtat tcgacgcccg ctacggcttt gatgccgctg
    33361 cctttgcggc acttagcgga acgttgaaag cgggaagctg gctggttttg ttactccctg
    33421 tatgggaaga gtgggaaaac caacctgatg ccgactcgct gcgctggagt gattgccctg
    33481 accctattgc gacgccgcat tttgtccagc atttcaaacg cgtacttacg gcggataacg
    33541 acgctatcct ctggcggcaa aaccagccgt tctcgttggc gcattttact ccccgtactg
    33601 actggcaccc cgcgaccggc gcaccacaac cagaacaaca gcaactctta cagcagttac
    33661 tgaccatgcc gtcgggcgtg gcagcggtaa ctgctgcgcg tgggcgcggt aagtcggcgt
    33721 tggcagggca actcatttct cgtattgcgg gcagtgcgat tgtcaccgcg cccgcaaaag
    33781 cggcaacgga tgtactggca caatttgcgg gcgagaagtt tcgctttatt gcgccggatg
    33841 ccttgttagc cagcgatgag caagccgact ggctggtggt cgacgaagcc gcagccatac
    33901 ctgcgccgtt gttgcatcaa ctggtatcgc gttttcctcg aacgttgtta accactacgg
    33961 tgcagggcta cgaaggcacc ggacgtggtt ttttgctgaa attttgcgct cgctttccgc
    34021 atttacaccg ttttgaactg caacagccga tccgctgggc acagggatgc ccgctggaaa
    34081 aaatggttag taatgcactg gtttttgacg atgaaaactt cacccataca ccacaaggca
    34141 atattgtcat ttccgcattt gaacagacgt tatggcgaag tgaaccagaa acgccgttaa
    34201 aggtttatca gttattgtct ggtgcgcact accggacttc gccgctggat ttacgccgca
    34261 tgatggatgc accagggcaa cattttttac aggcggctgg cgaaaacgag attgccgggg
    34321 cgctgtggct ggtggatgag gggggattat ctcaacaact cagtcaggcg gtatgggcag
    34381 gttttcgtcg cccgcggggt aatctggtgg cccagtcgct ggcggcgcac ggcagcaatc
    34441 cactggcggc gacattgcgc ggacggcggg tcagccggat agcagttcat ccggctcgtc
    34501 agcgggaagg cacaggacag caacttattg ctggtgcttt gcaatatacg caagacctcg
    34561 actatctttc ggtgagtttt ggttacaccg gggagttatg gcgtttctgg caacgctgcg
    34621 gttttgtgct ggtgcgaatg ggtaatcatc gtgaagccag cagcggttgc tatacggcga
    34681 tggcgctgtt accgatgagt gatgcgggta aacagctggc tgaacgtgag cattaccgtt
    34741 tacgtcgcga tgcgcaagct ctcgcgcagt ggaatggcga aatgcttcct gttgatccac
    34801 taaacgatat cgtgctttct gacgacgact ggcttgaact ggccggtttt gctttcgctc
    34861 atcgtccgct attaacatcg ttaggttgct tattgcgtct gctacaaacc agtgaactgg
    34921 cattaccggc gctgcgtggg cgtttacaga aaaacgccag tgacgcgcag ttatgtacca
    34981 cacttaaact ttcaggccgc aagatgttac tggtccgtca gcgggaagag gccgcgcagg
    35041 cactgttcgc acttaatgat gttcgcactg agcgtctgcg cgatcgcata acgcaatggc
    35101 aattttttca ctgactcctt caattttctc ccatggtcat tgtgagtcag cggcgtaaaa
    35161 ttaatgacat cagttaagga gttcgccatg aaacatgacc attttgttgt tcaaagcccg
    35221 gataaacctg cgcaacagtt gttactgctt ttccacggtg tcggggataa cccggtggca
    35281 atgggggaaa taggcagctg gtttgcgccg ctatttcctg atgcgctggt ggtgagtgtc
    35341 ggaggcgcgg agccgagcgg taatccggcg gggcgtcagt ggttttcggt gcagggtatt
    35401 acggaagata atcgccaggc gcgtgtggat gcgatcatgc cgacgtttat tgaaacggta
    35461 cgctactggc agaaacagag tggagtaggg gctaacgcca cggcgctaat tggtttctca
    35521 caaggcgcga ttatggcgct ggagagcatt aaagccgaac cgggccttgc ctcacgcgtc
    35581 attgccttta acggacgcta tgcgagcctg ccggaaaccg cgtctacagc cacgacaatt
    35641 cacctgattc atggcggtga agatccggtt atcgatctgg cgcatgcagt tgcggcgcag
    35701 gaagcgttaa tcagtgccgg tggtgacgtg acgctggata tcgtggagga tttaggccat
    35761 gcgatcgaca atcgcagtat gcaatttgcc ctggatcact tgcgttatac cattccgaaa
    35821 cattatttcg acgaagcgct aagcggcggt aagcctggcg atgatgatgt gattgaaatg
    35881 atgtaataat agccgggtag gcctgataag accgtatcag acatcatggc cggatgcggc
    35941 gttaacgcct tatccggcca ctcagcttac ttcttcggtt gatccttttt cggccagtcg
    36001 tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg
    36061 agaaatttct tatggtcaac gcgcttgaga tctttaatca cgttcagcag gacgcctacc
    36121 aaaaaaacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct
    36181 tgcagaacca ctcatcacgc gacgagctgt tccatgatac gttgatacat acgggcaagt
    36241 agctgcaggt cggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc
    36301 ccgagttcca ccacctgcgc ccccatgcgg gcaataaagc gcccgtcgga cgttccgcct
    36361 gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc
    36421 accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccaatc caccgtatag
    36481 cgcagttgat gtttttcaag cagggcaagc acctgcgctt tgatcatctc atcagtcagt
    36541 tcggtgctga agcggaagtt aaactgcaca aacagttcac ccggaataac gttgttactg
    36601 cccgttcccg cctgaatatt ggcaatctgc atactggtcg ccgggaaaaa ttcattgccc
    36661 tgatcccact caatagccac taattcatta aggaaaggtg ctgcgcgatg taccggattg
    36721 tcagccagat gtgggtaggc aacatgcccc tgaacgccat gaatggtaag gttgcaggtt
    36781 aacgatccgc gacgaccatt tttcaccaca tcacctacca cttcgatact cgacggttcg
    36841 ccaaccaggc agtaatcgag acgctcatta cgtgccatta acgcttcgac gacttttacc
    36901 gtaccgttgt gggcactggc ttcttcatca gaggtgatca gaaatgccag tcgccccgta
    36961 tggttgggat gttgtgcgac aaaacgttct gcagctacca ccatcgccgc cagcgagcct
    37021 ttcatatctg ccgcaccgcg cccgaataac atgccgtcac gaatggttgg ttcaaacggc
    37081 ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggct
    37141 aacgtttcac cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa gtccatgcgt
    37201 tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca tcctgcgtca
    37261 tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac
    37321 atagactaca cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata
    37381 ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc agtcattaat
    37441 gccgccgcag aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag tttacgccag
    37501 gttgtaccac gggtgttgag taacgcttcc cagcctaatt cgttgataaa accgttcaat
    37561 aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct
    37621 tccagccaac ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc
    37681 ataatgaatc ctttttcgat ttatgagaat aaatactcat ttaagggtga aaacattttg
    37741 cctgcaa
//
LOCUS       DAZGCQ010000043        37612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000043 DAZGCQ010000000
VERSION     DAZGCQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.043"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            215..1588
                     /gene="aroP"
                     /locus_tag="ACHK07_002346"
     CDS             215..1588
                     /gene="aroP"
                     /locus_tag="ACHK07_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO8102666.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHK07_002347"
     CDS             complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHK07_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO8102667.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHK07_002348"
     CDS             complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHK07_002348"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO8102668.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDISNNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3121..4014
                     /gene="nadC"
                     /locus_tag="ACHK07_002349"
     CDS             3121..4014
                     /gene="nadC"
                     /locus_tag="ACHK07_002349"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO8102669.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHK07_002350"
     CDS             4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHK07_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO8102670.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTIRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4667..6052
                     /gene="gspE"
                     /locus_tag="ACHK07_002351"
     CDS             4667..6052
                     /gene="gspE"
                     /locus_tag="ACHK07_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO8102671.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6042..7244
                     /gene="hofC"
                     /locus_tag="ACHK07_002352"
     CDS             6042..7244
                     /gene="hofC"
                     /locus_tag="ACHK07_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO8102672.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRFPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHK07_002353"
     CDS             complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHK07_002353"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO8102673.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8478..8522)
                     /locus_tag="ACHK07_002354"
     CDS             complement(8478..8522)
                     /locus_tag="ACHK07_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102674.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8547..9167
                     /gene="coaE"
                     /locus_tag="ACHK07_002355"
     CDS             8547..9167
                     /gene="coaE"
                     /locus_tag="ACHK07_002355"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO8102675.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9167..9910
                     /gene="zapD"
                     /locus_tag="ACHK07_002356"
     CDS             9167..9910
                     /gene="zapD"
                     /locus_tag="ACHK07_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO8102676.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9920..10117
                     /gene="yacG"
                     /locus_tag="ACHK07_002357"
     CDS             9920..10117
                     /gene="yacG"
                     /locus_tag="ACHK07_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO8102677.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10333..10722)
                     /gene="mutT"
                     /locus_tag="ACHK07_002358"
     CDS             complement(10333..10722)
                     /gene="mutT"
                     /locus_tag="ACHK07_002358"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO8102678.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10782..13487)
                     /gene="secA"
                     /locus_tag="ACHK07_002359"
     CDS             complement(10782..13487)
                     /gene="secA"
                     /locus_tag="ACHK07_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO8102679.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13549..14061)
                     /gene="secM"
                     /locus_tag="ACHK07_002360"
     CDS             complement(13549..14061)
                     /gene="secM"
                     /locus_tag="ACHK07_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO8102680.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14292..15209)
                     /gene="lpxC"
                     /locus_tag="ACHK07_002361"
     CDS             complement(14292..15209)
                     /gene="lpxC"
                     /locus_tag="ACHK07_002361"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO8102681.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15310..16461)
                     /gene="ftsZ"
                     /locus_tag="ACHK07_002362"
     CDS             complement(15310..16461)
                     /gene="ftsZ"
                     /locus_tag="ACHK07_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO8102682.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16522..17784)
                     /gene="ftsA"
                     /locus_tag="ACHK07_002363"
     CDS             complement(16522..17784)
                     /gene="ftsA"
                     /locus_tag="ACHK07_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO8102683.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17781..18611)
                     /gene="ftsQ"
                     /locus_tag="ACHK07_002364"
     CDS             complement(17781..18611)
                     /gene="ftsQ"
                     /locus_tag="ACHK07_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO8102684.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18613..19533)
                     /gene="ddlB"
                     /locus_tag="ACHK07_002365"
     CDS             complement(18613..19533)
                     /gene="ddlB"
                     /locus_tag="ACHK07_002365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8102685.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQADMSFSQLVVRILELAD"
     gene            complement(19526..21001)
                     /gene="murC"
                     /locus_tag="ACHK07_002366"
     CDS             complement(19526..21001)
                     /gene="murC"
                     /locus_tag="ACHK07_002366"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO8102686.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAKVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21055..22122)
                     /gene="murG"
                     /locus_tag="ACHK07_002367"
     CDS             complement(21055..22122)
                     /gene="murG"
                     /locus_tag="ACHK07_002367"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO8102687.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22119..23363)
                     /gene="ftsW"
                     /locus_tag="ACHK07_002368"
     CDS             complement(22119..23363)
                     /gene="ftsW"
                     /locus_tag="ACHK07_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO8102688.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23363..24679)
                     /gene="murD"
                     /locus_tag="ACHK07_002369"
     CDS             complement(23363..24679)
                     /gene="murD"
                     /locus_tag="ACHK07_002369"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO8102689.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADGRCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24682..25764)
                     /gene="mraY"
                     /locus_tag="ACHK07_002370"
     CDS             complement(24682..25764)
                     /gene="mraY"
                     /locus_tag="ACHK07_002370"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO8102690.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25758..27116)
                     /gene="murF"
                     /locus_tag="ACHK07_002371"
     CDS             complement(25758..27116)
                     /gene="murF"
                     /locus_tag="ACHK07_002371"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO8102691.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27113..28600)
                     /gene="murE"
                     /locus_tag="ACHK07_002372"
     CDS             complement(27113..28600)
                     /gene="murE"
                     /locus_tag="ACHK07_002372"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO8102692.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28587..30353)
                     /gene="ftsI"
                     /locus_tag="ACHK07_002373"
     CDS             complement(28587..30353)
                     /gene="ftsI"
                     /locus_tag="ACHK07_002373"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO8102693.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30369..30734)
                     /gene="ftsL"
                     /locus_tag="ACHK07_002374"
     CDS             complement(30369..30734)
                     /gene="ftsL"
                     /locus_tag="ACHK07_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO8102694.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30731..31672)
                     /gene="rsmH"
                     /locus_tag="ACHK07_002375"
     CDS             complement(30731..31672)
                     /gene="rsmH"
                     /locus_tag="ACHK07_002375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO8102695.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31674..32132)
                     /gene="mraZ"
                     /locus_tag="ACHK07_002376"
     CDS             complement(31674..32132)
                     /gene="mraZ"
                     /locus_tag="ACHK07_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO8102696.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32734..33738)
                     /gene="cra"
                     /locus_tag="ACHK07_002377"
     CDS             complement(32734..33738)
                     /gene="cra"
                     /locus_tag="ACHK07_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO8102697.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33820..33906)
                     /locus_tag="ACHK07_002378"
     CDS             complement(33820..33906)
                     /locus_tag="ACHK07_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO8102698.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33918..34409)
                     /gene="ilvN"
                     /locus_tag="ACHK07_002379"
     CDS             complement(33918..34409)
                     /gene="ilvN"
                     /locus_tag="ACHK07_002379"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8102699.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34412..36136)
                     /gene="ilvI"
                     /locus_tag="ACHK07_002380"
     CDS             complement(34412..36136)
                     /gene="ilvI"
                     /locus_tag="ACHK07_002380"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO8102700.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRSNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36454..37398)
                     /gene="leuO"
                     /locus_tag="ACHK07_002381"
     CDS             complement(36454..37398)
                     /gene="leuO"
                     /locus_tag="ACHK07_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO8102701.1"
                     /translation="MPEVQTDHPEMAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9087 a        10491 c         9167 g         8867 t
ORIGIN      
        1 gatttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
       61 tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt
      121 ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
      181 gtagatcaaa aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
      241 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
      301 agggaccggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat
      361 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
      421 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
      481 ctttgctggc ttcgcttctg gctggaacta ctgggtactg tacgttttag ttgccatggc
      541 agagctgact gctgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt
      601 ttctgccgcc gtgttctttg tggtgattaa cgccatcaac ctgaccaacg taaaagtgtt
      661 tggtgagatg gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat
      721 cttcggcggc tggctgctgt tcagtggtaa cggcggtccg caggcaagcg ttagcaacct
      781 gtgggatcag ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga tgatggcgat
      841 tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
      901 cccggagcaa agtatcccga aagcgactaa ccaggttatc taccgcatcc tgattttcta
      961 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
     1021 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
     1081 catcgtggtg ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
     1141 tatgctgttt ggtctggcac aacagggtaa tgcgccaaaa gcgctggcgt ctgtcgataa
     1201 acgcggcgta ccggttaaca ccattctggt gtctgcactg gtaacggcat tgtgcgtact
     1261 gattaactat cttgctccgg aatccgcatt tggcctgtta atggcgctgg tggtatctgc
     1321 actggtgatc aactgggcga tgattagcct ggcgcatatg aaattccgtc gcgccaagca
     1381 ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg
     1441 cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc
     1501 ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa
     1561 aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacctgta cacggtgagg
     1621 gcaataatct ttacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca
     1681 cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat
     1741 gcggttcacg cgccagagag aactgcgcca gacgcgttaa cacctgatac tgcgaagtat
     1801 ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg
     1861 catataccac acccgcaaga cgaactggca cccaatccag tacatgaagc acggcatcaa
     1921 tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat tgccatgcac
     1981 gcaagaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga
     2041 acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca
     2101 aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca
     2161 tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa
     2221 gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg
     2281 gaacgttgaa caatacgccc tgcaatgcgc gtaacagtaa aaaagtcacg cccatcgcaa
     2341 taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt
     2401 caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca
     2461 ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc
     2521 ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt
     2581 catgttatta gagatatccg gatagcaatc aatcagtgcc cgcgtaaccg ccgcaagctg
     2641 ttgatactgc gcatcggtat acgccagcgt atcggtgcct tcaagctcaa tcccaataga
     2701 aaaatcattg cagcgttcgc gcccctgata ctgagagact cccgcatgcc atgcacgttt
     2761 atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac
     2821 gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa
     2881 taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac
     2941 cagcagggtg ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg gaacgcggcg
     3001 cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa
     3061 tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac
     3121 atgccgcctc gccgctataa ccctgacacc cgacgtgacg agctgctgga acgcattaat
     3181 ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat
     3241 gccaacaatg acattacggc aaaactttta ccggaaaact ctcgctctca tgccacggtg
     3301 atcacccgcg agaatggcgt attttgcggc aaacgctggg ttgaagaggt gtttattcaa
     3361 ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc
     3421 aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact
     3481 gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa
     3541 ttactggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc cggcctgcgt
     3601 tcagcgctga aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct
     3661 gatgccttcc tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc
     3721 gaaaaagcgt cctggctgca cccggatgcg ccagtagaag tcgaagtaga gaatctggaa
     3781 gaacttgatg aagccctgaa agcaggagcc gatatcatca tgctggataa cttcgaaaca
     3841 gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc
     3901 aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc
     3961 ggtgcgctaa ctaagcacgt acaggcactc gacctttcaa tgcgttttcg ctagttgctg
     4021 acagatttat ccgctccata gtgggcggat aatcacccct tccccaactt tcttcgtaac
     4081 gcctcgcaaa tttcttgtct ttcgctgcaa cggaataaaa aacctcggaa gctgctttcc
     4141 cgtttctttt tttgcccctc gcttgtgctg atggcgtttg ccaaagtagc gccaacccaa
     4201 tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt
     4261 tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg
     4321 caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt
     4381 gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc
     4441 gcctaccacc atccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac
     4501 cgggcaagaa agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa
     4561 cggcgtcacc ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc
     4621 ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca
     4681 gctcacggcc ctgtgtctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt
     4741 tcatgttgcg gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac
     4801 caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg
     4861 cacacaacag acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct
     4921 gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc
     4981 ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt
     5041 ttcaccggat gccggagtcg cattaaccgc cagattaaaa gtgctgggaa acctggatat
     5101 tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacgccgt
     5161 ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt
     5221 acagcaggtg agccaggcac tggatgtcaa cacgctggga atgcagccgt tacaactggc
     5281 ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg gccctaccgg
     5341 cagcggcaaa acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa
     5401 tatttgtagc gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat
     5461 ccatccgcgt gccggactca cctttcaggg cgttttgcgt gcgttattgc gccaggatcc
     5521 tgacgtcatc atgatcggag agatccgcga tggcgaaaca gcagagatcg ctattaaagc
     5581 cgcgcaaacc ggtcacctgg tgttgtctac cctacacacc aattccacct gcgaaacgct
     5641 ggtacgttta cagcaaatgg gagtcgcccg ctggatgctc tcatcagcgc ttacgctggt
     5701 aatagcccag cgtctggtac gtaaactttg cccacattgt cgccagcagc aaggggagcc
     5761 catccatatt ccagacaatg tatggccgtc gccgctgccc cactggcagg cacctggttg
     5821 tgtacattgc taccacggtt tttatggtcg cacggcctta tttgaagttc tgcccataac
     5881 accggtcata cgtcagctta tttccgctaa taccgacgtt gaatcgctgg aaactcacgc
     5941 ccgacaggcg ggtatgcgta cgctttttga aaacggctgc ctcgccgtgg agcaaggctt
     6001 aactaccttt gaagagttaa tccgcgtact ggggatgccg catggcgagt aagcaactct
     6061 ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga
     6121 gccgtacttt actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc
     6181 gaatcgccat caattctgcg cagtggcggg gagataaaag cgcggaagtc attcatcaac
     6241 tggcgacgct actcaaagca gggttaacgc tttctgaagg gctggctctg ctggcggaac
     6301 agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg
     6361 gcattgcttt ttccaatgcc ttattaccct ggtcagaggt atttccgccg ctctatcagg
     6421 cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc
     6481 gtcagcaaaa agcccagcgt cagttgaccg acaaagtgaa atcagcgttg cgttatccca
     6541 tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg
     6601 agtttgccgc tatatataag accttcaaca ccccactacc ggcactaacg caggggatca
     6661 tgacgctggc agactttagt ggcgaatgga gctggctgct ggtgttgttc ggcattatgc
     6721 tgacgatagc caataagttg ctgatgcgcc gaccgacctg gcttatcgcg cggcagaaat
     6781 tgctgttacg cttcccgatt atgggttcac tgatgcgggg acaaaaactc acgcagatct
     6841 ttacgattct ggcgctgact caaagtgcag gcattacttt tttacagggc gtagagagcg
     6901 tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata
     6961 tcagtaacgg tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct
     7021 gtttgcaatt agtgagaacg ggagaggcct ccggctcgct ggatctcatg ttagacaacc
     7081 tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctagca gccttactgg
     7141 aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg gcgatgtatc
     7201 tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc
     7261 cacaccagcg ttggaagatt acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg
     7321 gataaatgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt atgtacaagc
     7381 tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag
     7441 cttaacggtt ttaccttctg ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat
     7501 cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat
     7561 gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc
     7621 gccgccaaaa gctttcgcga catcgcccgg cgtggtgcaa ccaccatcgc tgacgatcat
     7681 tccgcccaga ccgtgcgcag catcggcaca ttcgattacc gcagaaagtt gcggataacc
     7741 gacgcctgtt ttgacacgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat
     7801 atcggcaccg gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca
     7861 aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga
     7921 ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg
     7981 tttagttttt tcgaaatccg catcagacgt accggtagaa accatcacat gtttcagcac
     8041 atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt gtttatgcac
     8101 agcagtcaaa atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc
     8161 catatttgcg gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa
     8221 ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag
     8281 aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt
     8341 aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg
     8401 gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat
     8461 agtgagcgaa cgtttcatca atttcgcttt cgtgcgctca tttttaacgt tttgataatc
     8521 atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg ggaggcattg
     8581 gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg
     8641 ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg
     8701 atcactttgg cgctaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg
     8761 agcggatctt cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga
     8821 ttcagcaaga gacgcaacac cagatccagc aagcaacctc cccctatgta ctgtgggttg
     8881 tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg
     8941 tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg
     9001 tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg
     9061 tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac
     9121 actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt
     9181 cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat
     9241 tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg
     9301 taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa
     9361 agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca
     9421 gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatctccgc
     9481 gccgcgtatc gggcaatttc tgcgtgaaga tcgcttgatt gctctggtgc gtcagcgact
     9541 gagcatccca ggcggctgtt gcagctttga tttaccaaca ttgcacattt ggctgcatct
     9601 accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac
     9661 ccaggcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac
     9721 cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct
     9781 gtcgctcgat tcacagcttt atccgcaaat ttccggtcat aagagccgtt ttgccattcg
     9841 ttttatgccg ctggacagtg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc
     9901 ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa
     9961 aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct
    10021 gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc
    10081 cgaaagcgat gactggagcg aagaaccaaa gcagtgacat ttgtctgatg ccgcacgtag
    10141 gcctgataag acgcggacag cgtcgcatca ggcatcgtgt gcaaatgtcg gatgcggcgt
    10201 gaacgcctta tccgacctac aaaaaaaaat ttatcaatga gttagaaatc cctttaggcc
    10261 tgataagacg cgccagcgtc gcatcaggca tcgtgtgcaa atgtcggatg cggcgaaaac
    10321 gccttatctg atctacagac gtttaagctt cgcaattacc ggttcattgg ctggcggaaa
    10381 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    10441 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    10501 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    10561 aagttcacgc accaccgcct gttccggcgt ttcacccatt tcaattttac cgccgggaaa
    10621 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    10681 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    10741 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    10801 cactgcttgt attttttacc ggaaccgcac gggcaaggat cgttacgtcc tactttgcgt
    10861 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    10921 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    10981 tcttcaggca tacgtacctg aactttgctc agcgtactga taacttcata tttcaacgac
    11041 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    11101 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgctgc caggtgctct
    11161 ttccacaggg agtcaagcgt ttgcagcatg acgcctttct cgaagtgacg catcatctca
    11221 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aatgcgctca
    11281 cgcagcgtct cttcatgcag ttcgggttct ttatccagcc actcagcaat tggtaaatcg
    11341 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagc
    11401 gactgtggcg gaatgtaggc atcaatggtg gctttgaaca catcttcacg aatgctgttg
    11461 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    11521 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacga
    11581 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    11641 tcgcctggct tcatacccag tttacgcatc attccagata ctcggtcgga agcaaaaata
    11701 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    11761 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgata
    11821 atatgcaggc cacctgctgc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    11881 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    11941 ccgagcacaa tatctgtacc acgacccgcc atgttggtcg cgatagtcac cgcagccgga
    12001 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacattg
    12061 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    12121 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    12181 tgaatttttt ccgcttcagt catgtagacc aggtcaggca gatctttacg aatcattgga
    12241 cggttggtcg gaacaacgac ggtatccagt ttgtagatgg agctaaattc gaaagcttcg
    12301 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    12361 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    12421 gcctggtgca gaccatcaga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    12481 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cacgggtaaa cagcgcatga
    12541 gcgcgcagcg ccgccgttac gtgatgcatc agcatgatgt tggccggaga gtacagagac
    12601 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    12661 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    12721 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    12781 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    12841 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    12901 tcagggctga acgccatgtt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    12961 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    13021 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    13081 gccaggtagt cgttgacggt aactacatgc acgcctttac cggttagtgc gttcagataa
    13141 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    13201 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    13261 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    13321 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    13381 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    13441 cgatcgttac gactaccgaa aactttagtt aataatttga ttagcataat aaaatctcaa
    13501 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt agctgaggcg
    13561 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    13621 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    13681 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    13741 ttcttcagca acgggcagcg tttgcggtgc cattgcgaag gaaagatgac ggattaccgt
    13801 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgcgtgtt
    13861 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    13921 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    13981 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    14041 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    14101 acaaatctta tcattaaagc ctgaagtcca cagcacgaat gaagataagt tgtccgtaaa
    14161 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    14221 acgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    14281 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    14341 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    14401 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    14461 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    14521 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    14581 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    14641 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    14701 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    14761 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    14821 acaaatttct tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    14881 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    14941 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    15001 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    15061 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    15121 gggcgtaacg tcagggtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    15181 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    15241 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    15301 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    15361 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    15421 cagcggagcc atcccgtgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    15481 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    15541 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    15601 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    15661 gcccgccgtg atgttaacca gtacgccgcg cgcgccagac aggtcgatat cttccagcag
    15721 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    15781 agaacccatc attgcgtagc ccatttcaga cattacggtg cgtacgtctg caaagtccac
    15841 gttcatcaaa cccggacgag taatcagttc ggcgatacct tgcacagcgc ctttgagtac
    15901 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    15961 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    16021 ctccgcgaat gccatacgct tcttgccttc aaagttgaag ggcttagtga cgacagcaac
    16081 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    16141 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcag
    16201 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    16261 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    16321 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atgcgctcgc gcaccatgtg
    16381 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    16441 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    16501 tcattgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    16561 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    16621 ctctttccca tagtgaagca atcccaccgc cgttgaataa tacggctcct gagcataatc
    16681 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    16741 agcacaggct gcaagacctt cgatctgcgc tgcgccacca gttaatacaa tgcctgccgc
    16801 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    16861 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    16921 cagactccgc ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    16981 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    17041 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    17101 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    17161 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagacc
    17221 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    17281 cgccatatcg ttgtggcatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    17341 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    17401 cagcacacga tgctcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    17461 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    17521 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    17581 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    17641 tttatccata ccacgcgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    17701 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    17761 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    17821 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    17881 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    17941 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    18001 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    18061 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    18121 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    18181 gtatttcctt ccgcgtctac catgtgttga tcattccacc gcgcaatcgg cacatattca
    18241 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    18301 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    18361 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    18421 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    18481 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    18541 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca
    18601 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    18661 atctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    18721 ggcttccagc agataaaact gtccatcact gtccagcata acgtcaatac gtccccatcc
    18781 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    18841 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    18901 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    18961 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    19021 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    19081 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    19141 taactgctta tcgctaaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggtgc
    19201 gaccggtaaa ccagcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    19261 agatgccatc actccgctgc cggtataagg caagcccatc agctcgagca tcccctgcag
    19321 cgtaccatct tcaccgccgc ggccgtgtag cgcgataaac actttctgaa agcccatcga
    19381 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tatgcgtcaa taccgccttc
    19441 acgcagcccg gctaacaccg ctgcgccaga attcaaagaa acttcccgct cagcggaggt
    19501 cccgcccaac aggaccgcga ttttatcagt catgttgttc ttcctccgga gtttgcggct
    19561 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    19621 gaatcaggtc gttaccggtt aataccggtg ccagcatctc ggctaccttc gccggatccg
    19681 gcaccagaat gggatctatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    19741 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    19801 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    19861 gctgaaacag cattaccagg tttttatccg gccagcctgc acgcgccgct ttaatagtgg
    19921 cgtccacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    19981 cattcactgg ctccagcggg aattcaccaa ggaaatcaaa acggcgacca gtcccctgga
    20041 agctctccag cgcacgcaaa atagcctcgt cgtcaatgcc ctcttccgta gcaaccgcaa
    20101 ccgcagctgc ggcgttcagc gcgttgtgac gacctggtgc attcagggtg acgcgcatcg
    20161 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    20221 ctacacgcac gtcggcatct tcgctgaagc cgtaagttgt ggtctgacgt cccactcgcg
    20281 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    20341 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    20401 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    20461 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    20521 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    20581 agatgctgga gaccatcgcg gtagttgtcg ttttgccgtg cgttcctgca atggcgatgc
    20641 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    20701 cttcatgagc tgcgacaatt tccgggttat cggcagaaat agcgctggaa acaacgacca
    20761 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    20821 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    20881 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    20941 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    21001 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    21061 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    21121 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    21181 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    21241 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    21301 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    21361 cgcccacgca tacgccgccg ccatatcatc aataaattca gtcactttat gctgcggttg
    21421 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttgccgc tctgatgcca
    21481 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    21541 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    21601 ttgctgcggc aacggtagcg ccaacacatc ggtacgcacc gggttaccca ccacttccgc
    21661 attggggaaa gcacctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    21721 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    21781 cgcggccaga ccaccaggac ctgatacgta gccgcccata ccgagcacca cgtcaggttt
    21841 gtacgctttc ataatcgccc gcgcctgacg ccaggcattg aagatacgca gcggcgcagc
    21901 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    21961 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    22021 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    22081 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    22141 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    22201 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    22261 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    22321 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaatgcttt acggccaatc
    22381 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    22441 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    22501 agtttttgta ccgagttacc taaaccttgt ccccacagtt cgccgcgacc aaacgccatc
    22561 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    22621 gtaacacggc ggatacggta cggttcggcg aggatcagca acacaaccgc tgaaatgccc
    22681 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    22741 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    22801 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    22861 cctttacgca ccagatagtt ggcgatatag caaaacagcg acagttttgt cagctccgca
    22921 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctcccc
    22981 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    23041 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaataaacgc caaaatcaga
    23101 tagacaccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    23161 attgacgccg aggtcaccat gataaagcca atcgccgcga ggccgaaggt cagccacagt
    23221 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    23281 ccctttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    23341 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    23401 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agtagaacca
    23461 tatcgcccgg ctgaacacgc ggagccagca agcgcatcgc ctgttccata gtttcggttt
    23521 gttctgccac ttccgggcgt agcgccgcca gctgcacgcc gtcacgaccg aaacaataca
    23581 gacgtacgtt atcgccattc aggtaacgcg ccagtgggct aaagtccgcc gatttaccat
    23641 cgccacccag caacaaatgc agcgtgccgt ctacgtgcag gccattcagc gccgcttccg
    23701 tactgccgac gttggtcgct ttcgaatcgt taatccaacg tacgccgtta tgctccagca
    23761 caacttcaaa gcgatgcggc agaccagtga atgtggttaa cgctttcagg ctgctggctc
    23821 gcggtaaccc tgcggcatct gccagtgcca gcgccgccag cgcattggtg tagttatgct
    23881 gcccggaaat tttcatttct ttcacgttca gcactttctc gcctttcacc cgcagccagg
    23941 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    24001 gtccatccgc accgcgaatc ggcattgtta aggcatcatc agcattaacc acgcaaactt
    24061 tcgcgttttc gtaaatgcgc agttttgctg cacgatactg ttgtaaacca aacggatagc
    24121 gatccatatg atcttcagtc acgttcagaa tggtcgctgc taccgcctgt aagctggagg
    24181 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcacactca tcatccagta
    24241 gcatcaacgc aggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    24301 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    24361 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    24421 cagcggcagc gcttaaggat ggatgcgcca gtgcaatacc gggactggcg acaatcagat
    24481 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttcaacg gcttcgggta
    24541 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    24601 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acaacatttt
    24661 taccctggta atcagccatg attaacgtac cttcagcgtt gccagaccaa tcaggaccag
    24721 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    24781 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    24841 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    24901 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    24961 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    25021 cccgactatc gcggtacaga caataaccag ttccccggcg tgtcgcagat acggtatgtg
    25081 taaatagctg gcaaagttca tgttgccggt cgcccacgcc accagcgcaa aaccaccggc
    25141 gacaaatacg gtcggcataa ttgccagacc gtcaagacca tcggtcaggt ttaccgcgtt
    25201 gccagtaccc acaatgacga agtaagccag caaaatgtag aacagcccca gttgcggcat
    25261 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc caacaaggta
    25321 cagcgcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    25381 caaccctttg gtgtctttac gcactacttt gcgataatca tcaacaaagc caataacacc
    25441 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    25501 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    25561 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    25621 caaatgagca atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    25681 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    25741 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    25801 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    25861 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    25921 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    25981 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    26041 cccaccacca gcacgcggta gcccggcatt tcagccagta cctggactgc tgcggtcatt
    26101 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    26161 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcatcaagc
    26221 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    26281 cccggcaacg gcagcagaac atccacgcta ccggttgggg tttgcagggt aaattccgta
    26341 ccgtgcgaag tcacatggat attggtggcg gtgaaatcgc tgttggcggc attaggtgag
    26401 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    26461 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    26521 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    26581 gcagcttccg ggcgagtcag actcacagtc caggcaattt cgccctgatg gttcgcgcca
    26641 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acagcgtcat cggtacaccg
    26701 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    26761 gccgtcatct ctttaacgga ggttttgccg gaggaccccg tcagagcaac cacgcgcgcc
    26821 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    26881 attaactgcg gcaggtcgat gtccagcgga cggctaacca gtagtgcgcc cgcgccgcca
    26941 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    27001 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaaaggt gatatctgca
    27061 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    27121 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    27181 caatctggta atcttcatgg cctttgcccg cgaccagcac cacatcgttc tctttagcct
    27241 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    27301 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ctcttcggta cgcgggttat
    27361 cgtccgtcac caccgccaca tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    27421 tacctttatc gcgatcgcca ccacagccaa agacacacca cagcttgccc gcacagtgca
    27481 gacgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    27541 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    27601 cggcggtttt caacagatcc gccaatggat agcccagtgc caacagcgtc gccagcgcga
    27661 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ctcaatttcg ccatcgcccc
    27721 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg ttcgctttta
    27781 accagcgtcc gtgacagttc ggattgatat gatcttccat tgataccgca accgcgtccg
    27841 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    27901 aatgatgctc agaataaagc agccatttcg cggcttcgta gtgttccata tcaccatgat
    27961 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    28021 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgat
    28081 ccaccagccc cgccagctcg tgctgaacat cgactgccga accggtagta ttttctgtcg
    28141 ggatcacttt ccccagcaga ccgttaccaa cggtacccat taccgcgctg gtttcgccaa
    28201 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    28261 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    28321 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    28381 cgtcggtcgc ctcatctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct
    28441 gcgggatata tcgacgcccg tccgcctgat gacctactac agctacaaag agatcgcccg
    28501 ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca
    28561 cccacggagc aagaaggtcg cgcaaattac gatctgccac ctgtcccctc gccttgatta
    28621 atcacaaatt catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc
    28681 aatacgccgc ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta
    28741 cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca
    28801 acgcctgcgg tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc
    28861 gcggtaccgg ttttaatggc gatacgatag cctttaatcg ccgccttcac gccgccgccg
    28921 cccggtagcg ccacgctttc catcatatgc accacagtgc ggacaatgga ttccgggaag
    28981 acacgttcac cgggaaccgg ggggtcaact ttggtaatcg acagcgggcg ataaatgccg
    29041 tagctgccga tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag
    29101 ccgaaagaga aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca
    29161 ctgcgttctc cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta
    29221 tctactaacg ctgaggacgg catcgctaac gccagcttgg aaacaccgac gttactcgac
    29281 ttctgtaata ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg
    29341 ccgttaattc gataaggaat ggtattgagt accgagtttt cccgcaccac gccacgttgc
    29401 aacgcggtca tcaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg
    29461 gtacggttac gcatcgcctc ttttggcgtg ccgctcagat tatttgggtt gtatgatggg
    29521 ctgttagcca tcgccagcac ttcaccggtg ttgacatcca ccagcacggc gctaccggac
    29581 tcagccttgt taaaggccac cgcgttattc agttcgcgat aaaccagcgc ctgcaggcgt
    29641 tcatcaatac tcagcgccag gttgtgcgca gcctggctgt cagtagaaga aatatcttca
    29701 attacgcgac catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat
    29761 ttatcgaaac tcttctcgac gccctcaatc ccctgactat cgacgttagt aaagccgatg
    29821 aggtgagcag tcacttcgcc ggatggataa taacggcgag attcttcacg cagatgaatc
    29881 cccggcagtt tcagtttttt gatgtagtcc gccatgtcag ggtttacctg acgcgccaga
    29941 taaataaagc gccctttcgg gttggcgtta atgcgggctg aaagctgatc cagcggaata
    30001 ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc
    30061 acttctttcg ggtcagccca aatcgctttt accggcacgc tcaccgctaa cgggcgacca
    30121 gaacggtcag taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg
    30181 tcgccctctt tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc
    30241 agaaaagcca gcgccaggag aatacagccg cataacaacg caaaacgcca actgataaag
    30301 ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc
    30361 gtttatcctt atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc
    30421 agcttttccg tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg
    30481 atcaggttgc gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga
    30541 gcggtcagta aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg
    30601 caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacacca
    30661 ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc
    30721 actctgctga tcatgcgttc gtcctctctg caatacgcag aactgaacta cgggcacgag
    30781 gattctcagc cacctcttct tcgcccggca ttaacttgcc tagtgctcgc agctgacggc
    30841 cacccagttt tttgagctgc tcttcagtca tcggtaaccc tgccggaact tgcggaccgc
    30901 ggctgttttc acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg aagctgatga
    30961 tcgaaagccg cccacccggt gccagcacgt tgagcgagct ttttagcgcc tgctctatct
    31021 cctccagttc actgtttacc caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt
    31081 taaacttatc tttcaccggc gttgcagcag ccacgacttc cgccagttct ttggtgcggg
    31141 tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct
    31201 cttcaccata ggttttcaat acccaggcga tatcggcttc ttctgcggtt tgtagccatt
    31261 cagcggctga ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa
    31321 aggaaaagcc gcgttcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa
    31381 tgccgtcgat cttgccgata agatcgcgct cggcaacgta ttcgcccagc gcggaaaaag
    31441 gtccgtggat gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg
    31501 ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg
    31561 agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc
    31621 cattaacggc ttcatccaac agcaccgtag tatgtttata gttttccatc attttataga
    31681 gacaagtcct gcagtcgctc cgataagtct ccggtagcca actgctctgc gtcgatatct
    31741 tccttgacct gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca
    31801 accagcatca cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat
    31861 cgacctgcgc catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg
    31921 cgctcaaccg ggttcatgct cgacagacgc gataattttt gctcgataat ttcccattca
    31981 ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg
    32041 gcagcgttct caagcagctg ttcccgataa cgggtaggca ctgataagcg ccctttgctg
    32101 tcgagattga ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg
    32161 ccccacaatt tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc
    32221 acaaggcata ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt
    32281 acgtccaacg tgcgaagcaa attaacgtta tgaatagctg taagaaaaaa accctcatcg
    32341 tcatcaccgt tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg
    32401 acgcgagcgg cattttagga catatcttcc ccggttaaca gtccctgttg cgtctgtgta
    32461 gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa atcggcaatt
    32521 catctctgca tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt aattattgtg
    32581 aatttcagct ggtgagttaa ttaacgggcg ggtttaaaag catttatcaa taatagtcag
    32641 gaatagtctt atttacttta aggatattga tgtccagtcc cgtactctac gcgccagagt
    32701 gaaattcacc tggcgcgtat ttttgttcgc agcttagcta cggctgagca caccgcggcg
    32761 atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt cgtccaggct
    32821 tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag ccactgccag
    32881 caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc
    32941 agaaggcagt ttaccatcgc gacgtagcgt gacatccatc accccttgca acaacgcaaa
    33001 cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc agccattttt cgaataactg
    33061 ggcagccgcc tcccgctcat agctgttggc atacagaaaa tgcacttcgc gcggatcatc
    33121 tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct ccggcagcgc
    33181 gccaagataa agcaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc
    33241 atcatcctga tcggcaccaa ccacgctggt aaagtgttca cgatcgaggg cgcggtccag
    33301 cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct caggaggcaa
    33361 cgacgtcgaa acaataatgg catcgacctg acgctgtaaa aggtgctcaa tacagcgcat
    33421 ttcgttgtct ggctgatctt ctgagcaggc aatcagcagt tgataccccc gttgccgcgc
    33481 ctggcgttca agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac
    33541 aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta
    33601 attgtgctca cgcacgacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg
    33661 cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc
    33721 gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac
    33781 tggtatgggt taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt
    33841 attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg gctgtaaatt
    33901 gcgcattgag atcattctca acgcattatt ttatcgccgc gcgaaagtcc gaccacacca
    33961 gagcgagcaa cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa tgcatcaagc
    34021 ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc gataatttgc
    34081 ccacggaata tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt
    34141 ttcaccagca tgatttccct ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag
    34201 acatcgacca gcttgtgtaa ttgcttttcg atctgctcaa gtactttttc atcgcccacg
    34261 gtctggatgg tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca
    34321 atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt
    34381 tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca
    34441 tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcgctg ccatcgacgg
    34501 taacatcaac aaacaccagg cgattactgc gcacctgttc cagcgcctcg ctaagtttgc
    34561 tttccagctc atgcggatga gaaatctgga tcccgacatg cccataggct tccgccagac
    34621 ggacgaaatc gggtagcgat tgcatataag attgtgaatg acggccggaa tagatcatgt
    34681 cctgccactg cttcaccatc cccagatagc ggttattgag attcaccacc agtacgggca
    34741 actcgtattg caacgcggta gacagttcct gaatgttcat ctgaatactg ccgtcgccag
    34801 tgacgcaaac cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac
    34861 caaaacccat cgtgccgagg ccaccggaat tgatccagcg acgcggtttg tcgaatggat
    34921 aataaagtgc agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct
    34981 tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt
    35041 catatttcag gcactgacga gcgcgccact gttcaatttg ctgccaccag tcgcggatct
    35101 catccagtgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt tcgaggacct
    35161 ggcgagcatc ccccacaatc gggatatccg cagtcacggt tttagaaatg gaagtaggat
    35221 caatatcgat atgcagaaca gtggcatttg ggcagtactt tgccagattg ttcgtcgttc
    35281 ggtcatcaaa tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc gtcatattgg
    35341 cttcgtaggt accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg
    35401 cccccagccc catcaatgag gaaacaacgg gcagattcaa cgcctccacc gtttctttca
    35461 actgctgatg gcagcccgcc gtgattgccc caccgcctac gtagacaacc ggtttttttg
    35521 ccgctaccag cgtttgcaga gcacgcttaa tttgcccttt atgtccggta gtagtgggat
    35581 tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc gccggattaa
    35641 gaatatcttt cggtaaatca acgaccactg gtcctgggcg accacttgcc gccagccaga
    35701 aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa
    35761 ccaccggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg
    35821 aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa gcggtggcga
    35881 tgccagtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc
    35941 cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga cgaactaaaa
    36001 catgatcgat accacccacg gtatgcaatg catcgtaaat atcaaggact gcgcctccgg
    36061 gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc
    36121 cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta aaacacatta
    36181 accgtccgat cttgcttaag caattggcag ccagagacat aaacgccggg taataagaat
    36241 aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga taaaaatgat
    36301 aataaatcgg ctaaatccca ccacttacag caatttctct atttttttaa ttaatcctaa
    36361 aatagagagc ccacatattc agaataaaat tctacattca ataaaaaatg cagaataaac
    36421 cagacattca tgtctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg
    36481 ctcttccatc cactgatggc ctttatcgcg cccggcagct tcatgccagg agagataaca
    36541 ggttctgcta tttagtttta acggcagcgg taatacctgt aattctaagg attcagcgaa
    36601 ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag
    36661 tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata
    36721 ccacggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata
    36781 aacatcatgt ttcagtaacg ggcccttaat tgttgggtga tttttgctgg ctaccagcac
    36841 catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt cttcataact
    36901 aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga
    36961 cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt
    37021 cagaatacta tctaacgggc tgcaaacaca aagatgaaat acacgttcac tgctcgcggg
    37081 ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac gaactgaacc
    37141 aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc
    37201 gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacattcc
    37261 cagaacatga gcagcacggg taatgttttg ctcctgcatc acggcatcga aaacggttaa
    37321 taagttgaga tcgaccatgc gtagctgtgg cttgctgaac tccgccatct ctggatgatc
    37381 tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg
    37441 aatgcagaga aataatcctc gagaatatga tttatcatgc atataaaata agaaaaagga
    37501 aaatgagtaa aattaggaaa acataaagat acgggttctt ataaaaacag aatccattgc
    37561 cgtggaaagc gtcacttacc acagcataat aatccataat gtttttgttg cg
//
LOCUS       DAZGCQ010000044        37591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000044 DAZGCQ010000000
VERSION     DAZGCQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.044"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..334
                     /locus_tag="ACHK07_002382"
     CDS             131..334
                     /locus_tag="ACHK07_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102702.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            340..555
                     /locus_tag="ACHK07_002383"
     CDS             340..555
                     /locus_tag="ACHK07_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8102703.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            555..794
                     /locus_tag="ACHK07_002384"
     CDS             555..794
                     /locus_tag="ACHK07_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102704.1"
                     /translation="MRNTAKLQLGFSPLSKKIMLAKMRDVDGGRLRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEISWGLDDGSQMVLRAEKQVK"
     gene            795..1064
                     /locus_tag="ACHK07_002385"
     CDS             795..1064
                     /locus_tag="ACHK07_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8102705.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            1061..1237
                     /locus_tag="ACHK07_002386"
     CDS             1061..1237
                     /locus_tag="ACHK07_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO8102706.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(1206..1535)
                     /locus_tag="ACHK07_002387"
     CDS             complement(1206..1535)
                     /locus_tag="ACHK07_002387"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO8102707.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            1648..1824
                     /locus_tag="ACHK07_002388"
     CDS             1648..1824
                     /locus_tag="ACHK07_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102708.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            1885..2664
                     /locus_tag="ACHK07_002389"
     CDS             1885..2664
                     /locus_tag="ACHK07_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8102709.1"
                     /translation="MSERTLTLAGTGKQPMMSSREIAEVVNKNHADVCRDIRAMLAKL
                     YGGDERDYIRKADLLYLTNQCVNCLQYNTDNPNAWEYQLDYEHSECLVTGYDAVRRMA
                     MIKHWQKLKKEVEPPQLAITHPAPVIQVDSRIVQLARVIAEATASATMKAMSEVIALP
                     TYQAESAAPANLSHVEGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRSDTGVRGLLV
                     NLPAIEQAAQKLLNESTPPKGNRKRWTHPQFGNFTYYTDLI"
     gene            2709..4103
                     /locus_tag="ACHK07_002390"
     CDS             2709..4103
                     /locus_tag="ACHK07_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO8102710.1"
                     /translation="MGQIFTPRPYQDLIINHEIGISRCNIWAGMGMGKTVATLTTLED
                     LFMAGSETQPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            4100..4300
                     /locus_tag="ACHK07_002391"
     CDS             4100..4300
                     /locus_tag="ACHK07_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8102711.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(4297..5694)
                     /locus_tag="ACHK07_002392"
     CDS             complement(4297..5694)
                     /locus_tag="ACHK07_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8102712.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            5941..7005
                     /locus_tag="ACHK07_002393"
                     /pseudo
     CDS             5941..7005
                     /locus_tag="ACHK07_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(7105..9024)
                     /gene="dhaR"
                     /locus_tag="ACHK07_002394"
     CDS             complement(7105..9024)
                     /gene="dhaR"
                     /locus_tag="ACHK07_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO8102713.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            9252..10322
                     /gene="dhaK"
                     /locus_tag="ACHK07_002395"
     CDS             9252..10322
                     /gene="dhaK"
                     /locus_tag="ACHK07_002395"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO8102714.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFVTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            10333..10965
                     /gene="dhaL"
                     /locus_tag="ACHK07_002396"
     CDS             10333..10965
                     /gene="dhaL"
                     /locus_tag="ACHK07_002396"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO8102715.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            10976..12394
                     /gene="dhaM"
                     /locus_tag="ACHK07_002397"
     CDS             10976..12394
                     /gene="dhaM"
                     /locus_tag="ACHK07_002397"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO8102716.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNHQS"
     gene            12714..14411
                     /gene="treA"
                     /locus_tag="ACHK07_002398"
     CDS             12714..14411
                     /gene="treA"
                     /locus_tag="ACHK07_002398"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8102717.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHVPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGIENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQREVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSELTTQPVKQKEAEPTP"
     gene            14742..15764
                     /locus_tag="ACHK07_002399"
     CDS             14742..15764
                     /locus_tag="ACHK07_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO8102718.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSQ
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            15764..16744
                     /locus_tag="ACHK07_002400"
     CDS             15764..16744
                     /locus_tag="ACHK07_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO8102719.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            16741..17499
                     /locus_tag="ACHK07_002401"
     CDS             16741..17499
                     /locus_tag="ACHK07_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8102720.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            17509..18321
                     /locus_tag="ACHK07_002402"
     CDS             17509..18321
                     /locus_tag="ACHK07_002402"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8102721.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            18318..19172
                     /gene="modD"
                     /locus_tag="ACHK07_002403"
     CDS             18318..19172
                     /gene="modD"
                     /locus_tag="ACHK07_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO8102722.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            19198..21168
                     /locus_tag="ACHK07_002404"
     CDS             19198..21168
                     /locus_tag="ACHK07_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO8102723.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAVQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINVTTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21218..21472)
                     /gene="ymgE"
                     /locus_tag="ACHK07_002405"
     CDS             complement(21218..21472)
                     /gene="ymgE"
                     /locus_tag="ACHK07_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8102724.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            21673..22407
                     /gene="ycgR"
                     /locus_tag="ACHK07_002406"
     CDS             21673..22407
                     /gene="ycgR"
                     /locus_tag="ACHK07_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO8102725.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKARHITITAETQGAKVEFTVEQLQQSEYLQLPAFIS
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(22409..23020)
                     /gene="emtA"
                     /locus_tag="ACHK07_002407"
     CDS             complement(22409..23020)
                     /gene="emtA"
                     /locus_tag="ACHK07_002407"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO8102726.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            23120..24034
                     /gene="ldcA"
                     /locus_tag="ACHK07_002408"
     CDS             23120..24034
                     /gene="ldcA"
                     /locus_tag="ACHK07_002408"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO8102727.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPICRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTHEGTQLTISGHPVLKM"
     gene            24129..25865
                     /gene="cvrA"
                     /locus_tag="ACHK07_002409"
     CDS             24129..25865
                     /gene="cvrA"
                     /locus_tag="ACHK07_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO8102728.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            25986..26147
                     /locus_tag="ACHK07_002410"
     CDS             25986..26147
                     /locus_tag="ACHK07_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102729.1"
                     /translation="MDDKPLQALANKLKTPAQESQRNGFFRLQQVKNNQPRITGIPVN
                     HQGPQPDIA"
     gene            26486..27922
                     /locus_tag="ACHK07_002411"
     CDS             26486..27922
                     /locus_tag="ACHK07_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102730.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIVDLSIAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENQF
                     KTVRVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQQGLEDLLDFLRKEGQIDLE
                     GLEELEDLDDLMDLEFLDDPELLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSE
                     HEPEPASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(28091..29161)
                     /gene="dadX"
                     /locus_tag="ACHK07_002412"
     CDS             complement(28091..29161)
                     /gene="dadX"
                     /locus_tag="ACHK07_002412"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO8102731.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(29171..30469)
                     /gene="dadA"
                     /locus_tag="ACHK07_002413"
     CDS             complement(29171..30469)
                     /gene="dadA"
                     /locus_tag="ACHK07_002413"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO8102732.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            30799..32331
                     /gene="ycgB"
                     /locus_tag="ACHK07_002414"
     CDS             30799..32331
                     /gene="ycgB"
                     /locus_tag="ACHK07_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO8102733.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(32383..33102)
                     /gene="fadR"
                     /locus_tag="ACHK07_002415"
     CDS             complement(32383..33102)
                     /gene="fadR"
                     /locus_tag="ACHK07_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO8102734.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            33324..34865
                     /gene="nhaB"
                     /locus_tag="ACHK07_002416"
     CDS             33324..34865
                     /gene="nhaB"
                     /locus_tag="ACHK07_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO8102735.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            35011..35541
                     /gene="dsbB"
                     /locus_tag="ACHK07_002417"
     CDS             35011..35541
                     /gene="dsbB"
                     /locus_tag="ACHK07_002417"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO8102736.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(35587..36855)
                     /gene="umuC"
                     /locus_tag="ACHK07_002418"
     CDS             complement(35587..36855)
                     /gene="umuC"
                     /locus_tag="ACHK07_002418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO8102737.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(36855..37274)
                     /gene="umuD"
                     /locus_tag="ACHK07_002419"
     CDS             complement(36855..37274)
                     /gene="umuD"
                     /locus_tag="ACHK07_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO8102738.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         9308 a         9713 c         9423 g         9147 t
ORIGIN      
        1 cacctgacac agaagatttc aacgataaag agctttccgc gcttctctcc aataaccccg
       61 aatgggcgcc cgatatccca ctgaacgctg gcggttttga ggcgtaccac taccgtaagg
      121 attaatcgct atgactgtaa tcaaaacgca taccggaacg gttattaccc gcaccggtga
      181 aaagcaggtg caattgcatc aaaacgcgac aacgtgggtg gctggcccaa aagagtatta
      241 ctacaaagac actggccgcc gcggtggtgc tgtcggtgtc cgcactcgtc tggtgctgag
      301 cagcatccgc ccaatcgaag caaagcagga gtaaccccta tgtcatttaa atatcgggac
      361 aatccacttt attacagggc tgcgagggag gccttgcgcc ttgagcagtc cggcgaatat
      421 gaccgggcag cgaaggtctg ggccaaggct aaccgccagt cacgtaacga actgaatcag
      481 gactggagcg aacgccggtc tgatttttgt ctgatgcaga acatgcgcga aaagcgtaag
      541 gcggtcgaag aatgatgcgt aatacagcga aactacaact ggggttctcc ccgctatcca
      601 aaaagatcat gttggcaaaa atgcgcgatg ttgacggcgg tcgcttgcga gttggcaatg
      661 accctgggcg tgacgtaacg aacgaagccg cgcagttagt ttggcaactg gtaatggcag
      721 aaggcggcga aattagctgg ggtcttgatg atggtagcca gatggtactg agggctgaaa
      781 agcaggtgaa gtaaatggcc tacgaacgtg aaagccttat cgaaaagcac ctcgtcgccg
      841 aagtgaaaaa ggctggcggg gtcgccttta agttcgtgtc acccggtcgc cgctcggtac
      901 cggatcgcat tgtcctgcta cccggcggtc gcatcgtctt tgtcgaatgc aaagcgccag
      961 gcaagccacc acggcctgac cagttgcgcg agcatgaacg tctgcgaaag ctgggcttta
     1021 ccgtggtggt gctggatagc aaaaatctgg agggaatatt gtgagtaaac gtgatgaccc
     1081 acaattgcgg gtacgcatcc cgcaagactt gaaagacaca ctggaaaaaa tttccagaga
     1141 aaacgacaga acactaaccg ccgaaatcac ccggcggtta cgtaaaagtt tagaggatga
     1201 atccactatt cattctcagg atttagcaac ttcttaagtt tttccatcac ttcaataagc
     1261 tcgtatttgt tttcataaac ccattcgacc tgatactctc gatggtacgt tgtcttaagt
     1321 ttctcgagaa gatccctgag tatgagaatc tcatcagcag cttcctcaaa ccccctagat
     1381 gtatgaagta atttgtcctg aagaatagta gattccaacc ggctaacgat ctcagaggtc
     1441 agtgttctct tattagcccg tgctttcttc tccaaagcct cttttagcaa ttctggtatg
     1501 cgtaccctca actgcgggtc gtctcttttg ctcatatatg atcccttgaa agtgtgagaa
     1561 caacatgcct cacaaacatg ttgacttcaa gcctcattgt gagtactaat aaagtcctca
     1621 caatgagtac aaaacaaggt taacgctatg gctattcaga tcacgcagtt acatcaggca
     1681 gatattttcg ggtatatcgc ggacatgctg gaaacagcaa gacttctcag ttcgctggaa
     1741 aaaggagagc agttagcttt tgaattgatt tattttgcac agcaggccgc aagggaggct
     1801 gcaaacaagc cgtgggatga ataaacaaaa cgcgctggta gctgcaacta tcagcgcatc
     1861 ttttactgaa tggagaacac ttcaatgtcc gaaagaactt taacactagc aggcactggt
     1921 aagcaaccga tgatgagcag tcgcgagata gcagaagtgg taaataaaaa ccatgccgat
     1981 gtttgccgtg atattcgtgc aatgctggca aagctttacg gcggtgatga gcgcgattat
     2041 atccgtaaag ccgatttgct ttaccttacg aatcagtgtg ttaattgcct gcagtacaat
     2101 accgataatc caaacgcatg ggagtaccaa cttgactatg aacactccga atgccttgta
     2161 acaggttacg atgccgttcg tcgtatggcg atgattaagc actggcaaaa actgaaaaaa
     2221 gaagtcgagc ccccccagtt agctatcact caccccgcac ctgtaattca ggtggatagt
     2281 agaattgtcc agcttgctcg cgtcatcgcg gaggcaaccg catcagcaac catgaaagcg
     2341 atgtcggagg tgattgccct tccgacatat caagcagaat cggccgcacc agcgaattta
     2401 tctcatgttg aaggtgaata tgtccccgtc agcaaagcag catggaaaac aggtttatct
     2461 gattcaacat gccggaaatt gatcggcttc gccaggatac cggtacgttc agacaccggt
     2521 gttcggggat tgctcgttaa ccttccggcg atcgaacaag cagctcaaaa gctgttaaac
     2581 gaatcgacac cgccgaaagg taaccgcaag cgctggacac atccgcagtt tggcaacttc
     2641 acctactaca ccgatctaat ctgacattat tgcgcgggat taccccgcgc cctactggct
     2701 aaaatactat gggacaaatt ttcacgcctc gcccctacca agatctaatt atcaaccacg
     2761 aaataggtat ttcccgctgc aacatctggg cagggatggg gatgggtaaa accgtagcga
     2821 cactcaccac gctggaagat cttttcatgg cgggctcaga gacacagccc gcgctggtcc
     2881 tcgcgccgct acgcgttgca gcaagcacat ggccggatga agcgctgaaa tgggggcatc
     2941 tgcgcaatat cgaggtgcag ccgattgttg gtaacgccaa agcgcgctct gcagcgctgg
     3001 cgaacagcaa cgcgagcgtg ttcaccatca actacgataa ccttgtctgg ctggttgagg
     3061 aattgggaga acgatggccg ttcggtactg tcattccaga tgaaagcacc cggctaaaat
     3121 ccttccggct gcgaggtggt ggtaagcgcg cggcggcgct gggaaaagtg gcgcataagt
     3181 atgtccagcg ctggataaat ctcaccggta cgccagcacc gaacggcctg gtagatttgt
     3241 ggggacaatt gtggtttgtg gaccaggggc aacgtctcgg gcgcacttac ggcgcgttta
     3301 cctcacgctg gttcaactcg atacagtttc cggggcagag ctggaccaaa ctggagccgt
     3361 ttgctcactc acaggctgaa atacagcgag cgttagccga tgtgaccctc tcgctggatg
     3421 cggccgactg gttcgatatc aaagacccca tccataacgt aatccgcgtg gatatgccgc
     3481 cgaaggcccg tcagcagtat cgtgaaatgg aaaaggaaat gttcctcgag ctgaatggcg
     3541 aaggcatcga agcaccgaac gccgcggcaa agacactgaa gtgtcttcaa atcgccagtg
     3601 gcgcagtata cacagacgac accggaagtt ggtcagaact gcatgacacc aaactacagg
     3661 cgctggacag catactgacc gaagcagctg gcgcacctgt gctggttgct tatcactgga
     3721 aacacgatct tgaacgcttg cttaaagcat tccctcgcgg tcgtcacctc gaccaggatc
     3781 cacagacact gcgcgactgg aattccggaa agattcctgt tctctttgca cacccagcca
     3841 gcgcgggcca cggcctgaac atgcaggacg gcggaaatat actggtattt ttctcacact
     3901 ggtgggacct ggagcaatat cagcaaatta ttgaacgtat cggccccacc aggcagatac
     3961 aggccggaca caatcgtccg gtatttattc accacattat cgccgccgac actatggacg
     4021 aaatggtgat ggaacggcgc aactcaaaac gaacagtgca ggacatcctg ctcgatgcca
     4081 tgaaaaagag aggtatagca tgagcgagaa acccgacgat ttactcaccc cggatgaagt
     4141 atgccaaaag ttaggtatta cacagaaaac gctatgtgag tggaatatta agcatcgtca
     4201 tcgggctatc ctggcaccaa ttcgtttcag tgcaaaagta gttcgttatg agcgccgaaa
     4261 tgtcgacgct tttattcaaa aatgtcgcag ccagtattaa cctcgccgcc gtagcaatgc
     4321 cacctgcgca agtatgctcc gctcgtgagc ctcgaaagct tcgcgcttca acgcaatctc
     4381 ttcctgtaaa atctcatcag aaaagtcgta atgttctgcc atcgggtcat ctgacttgct
     4441 ggagtggtga aggcaaagga ggctgatttc ccttcggtct gagcgggaat agcctctttc
     4501 cttcatcaag gcaataacat tgctcttaag gaatttacgg cacatcgtat taaatgcacc
     4561 gtctttccct ttaacagtcc catcatgttt tattcctttt acagccccgt ccgggctgta
     4621 tgttttcacc agcttatcca gtgatcgttt tgaaaatggc tgcattggat cacgtggctg
     4681 caaaaataca taatccctgt tgcactcagg aactgaatca cgccaggctt tctgctcgtc
     4741 gataatccgc cggatctcag gcgttatcgg caggcggaaa gccttttgtg ttttcatagc
     4801 ccctcgcatg ccgataaccc cttccggata aacaatttca ccagcctcct cgtgaacgta
     4861 gtcccagcgc aggttatgga cattaatcgg acgaacacca gtgatgatca tgaagcgaac
     4921 agcattcttc tggtgtacag aggtgcaggc agcaacattg agccagagtc gggcgattga
     4981 ttcaatatcg gtaaaaagcc gtgtgggggt gggtttctgt acgcgggagg aaacataatc
     5041 atctggcaga ctggcggcaa cattgcggcc attgcaaaga gtaggtgcgc agaacttcca
     5101 gaaccgacgg agctcggcaa acaactccag ggcgttattg ttcgagcgag tggcgatcca
     5161 ctcgtccagc acttccacca gccgattgta tgttacgtcg ctgaacacct cgcgctcgcc
     5221 gaacgtggct ttaatccggt cgatacgcac cccgtaggtt gtgaaactgt ccgggctcag
     5281 cttctgccgg gcgactttgg ctttgaggtc atcccggtac atttccagcg ctgcatgtac
     5341 ggactctgcc cgcaggccac cgtcagccat acctagcgct ttttcgcgcg ccagctggat
     5401 agcgagctcc ggccactcgc cgagcttttt acctttgagg cccatctttt ttggaaactc
     5461 ggcataaaat gtaaccttac cggctttgct gaaatcgata cggagatagt tctctttttc
     5521 gtatttggaa cggcgagcca cgccggaagc agcgaggatg attttagcgg cagcaacaca
     5581 gattttcatg tgtgcgctgg tatagggggg tttacaggcg tcccattttt cagatgcggc
     5641 taaaacatcg tcattattgg ggctatccgg attatgtgtt acagtgcgcg gcattctcaa
     5701 tccttatctg cgtaggcgca gaaaacaagc tcacacatac aagtcttttc tacgggacaa
     5761 aatgcaatgt gttgcggttt tgtgttgctg gactgagttt atcaaggtta aatacactgg
     5821 atcaacatac agtaagttaa tgacagtaaa gcatacaaac tcgatacaac ttactgattt
     5881 taaaatgatt taacggtaat ccattgaaat gtctttacta attactaaac gctgtattaa
     5941 ttgtacgatg tgcgtaaaaa tggcactaac tgggagcttt atgcttccgg gacaattccg
     6001 gagccgactc caaatcctga accaacgccg gctcccgctc agcctcccat agtcaacccc
     6061 gatccaacgc ctaaccccac tcccacacct aaacccacca cgaccgcaga tgctggcggc
     6121 aattatctca atgtcggtta cttattaaac tatgttgaaa accgtacgct gatgcaacga
     6181 atgggtgacc tgcgaaatca gagtaaagac ggtaatatct ggcttcgcag ttatggggga
     6241 agcctggact ccttcgccag tggcaaactg agcggctttg acatgggtta cagcggtatc
     6301 cagtttggtg gggataaacg tctctctgat gtaatgccgt tgtatgtcgg tctgtatatt
     6361 ggctcaacac atgcatcgcc ggactatagc ggtggcgacg gtaccgcacg ttcagactac
     6421 atgggaatgt acgccagtta catggcacac aacggttttt acagcgatct cgttgtaaaa
     6481 gcatcgcgcc agaaaaatag tttccacgta ctggacagtc agaacaacgg cgttaacgcc
     6541 aacggcactg cgaatggatt gagcatctcc ctggaagccg ggcagaggtt caacctgacc
     6601 cctactggtt atgggttcta tatagagccg caaacccagc tcacatacag ccaccagaat
     6661 gagatggcta tgaaggcgag taatggcctc aatatacatc tgaatcacta cgaatcgctg
     6721 ctggggcgtg ccagcatgat actgggatat gacatcaccg caggtaaaag ccagctgaat
     6781 atctatgtga agactggcgc tatccacgag ttttcagggg atacagaata tctgttgaac
     6841 aactcccggg agaagtacag cttcaaaggt aatggctgga ataacggcgt gggagtcagt
     6901 gcacagtata acaaacagca cacattctac ctcgaagcgg attacacgca gggtaacctc
     6961 tttgatcaga agcaagtcaa cggaggatat cgcttcagct tttaagtgcg ggatagacaa
     7021 gcccttaaac gatgctgaaa aatgttagcg tgcgccggaa tgaaatatag atcactctga
     7081 tgtatcagaa atcgtttctg tctttcatac ccggcgctta aactgccctg catcaatgcc
     7141 atgttgcttc attttccgcc acagcgtagt gcggccgatt ccaagtaaag ccgacatttc
     7201 ctgaatgcga ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc
     7261 aaatgacaga ctggtggaaa ggcgtgtggc gctgacatca tctgttgcct gctcggtaaa
     7321 cagatgttcc ggcaaatcac tgacgcgaat gcgcccatta tcactactca gagcaagatt
     7381 ctcgatgacg ctgtaaagtt caaaatcgtt gccaggccat gcacaggaaa ccaggcgagc
     7441 gagggcatcg tcatcaattt tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt
     7501 gttattcacc agagccggaa tgctgccacg ccgcatacgc agaggcggga tggtaatttc
     7561 aaatgcatgc agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc
     7621 gaggtccgcg gtcgttgtag caatcacttt gacatcaatt ggtattaaac gccgcgcatc
     7681 cagtcgcgtg ataacaccct gcttgataac ctgaagcaaa gcagactgta actccaccgc
     7741 cagatattca atcttttcaa gaaacagcgt gccgccgtgc gccagttcca gccgactcag
     7801 acggccattt tcattgtccg tgcgatcgcc gccaataaat tcttccgcca gcaccgcatc
     7861 accatataac tcacaattga cggcgatata aggacctgca gcacgctcgc tttcattatg
     7921 aattgcctga cttagcagtg ccttgcccac gccttcttct ccacaaagca ggacaggaaa
     7981 gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg
     8041 tggcatatga gcgaaggtat ggctgacttt tcctaattga ctggtcatta actgccgcat
     8101 ctgttccaca ggatggagca acaaaataaa gctggttccc tgcgtttcta ttatcggttt
     8161 aagggttatc accgcatcaa taaactggtg ctggctttca aaggttgctt ctacgtgttt
     8221 gagcggatgt gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt
     8281 gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag acccgcgccg cctgggcatt
     8341 aataaattgc aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa
     8401 cagggcatta agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag
     8461 taaattaccg acctcgcggg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg
     8521 ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg
     8581 cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt
     8641 cacggcctga ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc
     8701 gcaatacgtg ccgtcatgga atccaagtgc acttagctgc tgcaaggttt gcggatcgcc
     8761 attacggctg agaatgcagg cggtttcatc gagaataaac agcgcacact ctcgcggtgc
     8821 catatattcc caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg
     8881 ccgataaata gaagcaaatg tcacgccctg ggcctgatgt ggtacgttcc atgtctcccg
     8941 tttcatcagc ttattgcatc gctcccagga ggttgcaatt aagggagata tgccccgacc
     9001 atcgttgtta aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc
     9061 tcaattttaa gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg
     9121 tttcactatg aaacataatt tgggctaatt ttcttttccc ttgcctgatg cacaatggat
     9181 tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct
     9241 ggagcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg
     9301 caggactggc gaaagcgcat ccatcgctga cactgcatca ggatccggtg tatgtcaccc
     9361 gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg
     9421 agccgatgca ctgtggttat atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa
     9481 ttttcacctc accgacgccc gataaaatct ttgaatgcgc catgcaagtt gatggcggcg
     9541 aaggtgtact gttgattatc aaaaattaca ccggcgatat tcttaacttt gaaacagcga
     9601 ccgagttact gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg
     9661 cagtaaaaga cagtctttat accgccgggc ggcgcggcgt tgccaacacc gtattaattg
     9721 aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg
     9781 ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc
     9841 ctgccgcggg caaaccttct tttaccctgg cggacaatga gatggagttt ggcgtcggca
     9901 ttcatggtga gccgggtatt gaccgtcgcc ccttctcttc ccttgatcaa accgtcgatg
     9961 aaatgttcgt caccctgctg gaaaatggct cataccatcg cactttgcgt ttctgggatt
    10021 atcaacaagg cagctggcag gaagaaccac aaaccaaaca accgctccag tctggcgaca
    10081 gggtgattgc gctggttaac aatcttggcg caactccact ttctgagctg tacggcgtct
    10141 ataaccgcct gaccacacgt tgccagcaag cgggattgac tatcgagcgt aatttaattg
    10201 gcgcgtactg cacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg
    10261 acgaaacgct ggcactctgg gacgccccgg tccacacccc ggcccttaac tggggtaaat
    10321 aaggagaaag caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc
    10381 gatattttca acaccgagag cgagtatctt accggactgg atcgcgagat tggcgacgct
    10441 gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg tggaaaaact ccctgctatc
    10501 gcagataaag atatcggttt cattctcaag aataccggta tgacgctgct ttccagcgtc
    10561 ggtggtgcca gtggtccgct attcggtacc ttctttatac gcgccgcaca ggcgacccag
    10621 gcacggcaaa gcctgacgct ggaagagctt tatcagatgt tccgcgatgg cgcggacggc
    10681 gtaatcagtc gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg
    10741 gtggtggaat cgttacgtca gtccagcgag caaaatctct ctgttccggc ggcactcgaa
    10801 gctgccagta gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa
    10861 ggccgcgcca gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcaacctcg
    10921 gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt
    10981 aaacctggtc atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg
    11041 tcagatgtta atgagtgata gttgtaaaat cgccattgcc gcgggaattg acgatccaca
    11101 aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc atcgaatctg ttgccgatgc
    11161 cgaccatgtg ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct
    11221 ggaattgctg gctcccgaga tcgctgcaaa agtacgtttg tgtgctgcac cgttggtcga
    11281 aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg gatatcgaca aagttatctt
    11341 tgacgccatg catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac
    11401 tgaaatctct gacacatgtc ctccgtacga tgaagaagcc cgttctctgg cggtggtcat
    11461 aaagaaccgt aacggcctgc atgtacgtcc agcctcccgg ctggtttata ccttatcgac
    11521 atttaatgcc gatatgttgc tggagaaaaa cggcaaatgc gtcacaccgg agagtattaa
    11581 ccagattgcg ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaagggcc
    11641 agaagctgaa gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac
    11701 ggaggaagtt gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcatttta
    11761 ttatcaacca gttttatgta cggtacaggc caaatcaacc ctgaccgtgg aagaggaaca
    11821 agatcgatta cgccaggcca ttgacttcac gttattagat ctgatgacgt taacagcgaa
    11881 agcagaagcc agcgggcttg acgatattgc cgcaatcttt tctggtcacc atacactgtt
    11941 agatgatccg gaactgctgg cggcggcaag cgaactcctt cagcacgaac attgcacggc
    12001 agaatatgcc tggcagcaag ttcttaaaga acttagccag caataccagc aactggatga
    12061 tgaatatcta caagctcgct atattgatgt ggacgatctt ctgcatcgca ccctggtcca
    12121 cctgacccaa acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga
    12181 gaacatttat ccctccgcag tactgcaact ggatccggcg gttgtaaaag gtatctgcct
    12241 tagcgccgga agtccggtat cccacagcgc cctgatcgcc cgtgaactgg ggattggctg
    12301 gatttgccag cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga
    12361 cgttaaaacg caacgtttca accatcagag ttaaaccacc gttcggatgg catcgttctg
    12421 atgtcatccg gcaatttact cagcactaat gttttttaac tacctgttat atttttcacc
    12481 aatttatgat gatttttctt cgctaatcgc gaaccaaacg ccgctggctg gctaataaac
    12541 agacgttatt tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa
    12601 tgcctgacag ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg
    12661 ttttctgata cttgaatacc gttttattcg gtttcgccta aggagaatga ttgatgaaat
    12721 cccccgcacc ttctcgcccg caaaaaatgg cgttaattcc agcctgcatc tttttgtgtt
    12781 tcgctgcgct atcggtgcag gcagaagaaa caccggtaac gccacagccg cctgatattt
    12841 tattagggcc gctgtttaat gatgtgcaaa acgccaaact ttttccggac caaaaaacct
    12901 ttgccgatgc cgtgccgaac agcgatccgc tgatgatcct tgctgattat cggatgcagc
    12961 aaaaccagag cggatttgat ctgcgccatt tcgttaacgt caatttcacc ctgccgacag
    13021 agggcgagaa atatgttccg ccagagggac agtcactgcg cgaacatatt gacggacttt
    13081 ggccggtatt aacgcgttct accgaaaaca ccgaaaaatg ggattctctg ttaccgctgc
    13141 cggaacctta tgtcgtgcca ggcggacgtt ttcgcgaggt atattactgg gacagttact
    13201 tcaccatgtt aggacttgcc gaaagcggtc actgggataa ggtcgcggat atggtggcca
    13261 attttgctca tgaaatagac acttacggtc atgttcccaa cggcaaccgc agttactatt
    13321 taagccgctc gcagccgccc ttctttgcca tgatggtaga gttactggcg cagcatgaag
    13381 gcgatgctgc attgaagcaa tacctgccgc aaatgcaaaa agaatatgct tactggatgg
    13441 acggtattga aaacctgcaa gccggacaac aggaaaaacg cgttgtaaaa cttcaggatg
    13501 gtacccttct caaccgctac tgggatgatc gcgatacgcc acgaccagag tcatgggtgg
    13561 aagatattgc caccgccaaa agcaatccga atcgacctgc cactgaaatt taccgcgacc
    13621 tgcgctctgc cgctgcgtct ggctgggatt tcagctcgcg ctggatggac aacccgcagc
    13681 agttaaatac cttacgcacc accagcatcg taccggtcga tctgaacagc ctgatgttta
    13741 aaatggaaaa aatcctcgcc cgcgccagca aagctgccgg agataacgcg atggcaaacc
    13801 agtacgaaac gctggcaaat gcccgtcaaa aagggatcga aaaatacctg tggaacgatc
    13861 aacaaggctg gtatgccgat tacgacctga aaagtcataa agtgcgcaat cagttaaccg
    13921 cggccgccct gttcccgctg tacgtcaatg cggcagcgaa agatcgcgcc aacaaaatgg
    13981 cgacggcgac gaaaacacat ctgctgcaac ccggcggcct gaacaccacg tcggtgaaaa
    14041 gtgggcaaca atgggatgcg ccaaatggct gggcaccgtt acagtgggtc gcgacagaag
    14101 gattacaaaa ctacgggcaa agagaggtgg cgatggacat tagctggcac ttcctgacca
    14161 atgttcagca cacctatgac cgggagaaaa agctggtgga aaaatatgat gtcagcacca
    14221 ccggaacggg gggcggcggt ggcgaatatc cattacagga tggctttggc tggaccaatg
    14281 gcgtgacgct gaaaatgctg gatttgatct gcccgaaaga gcaaccgtgt gacaatgttc
    14341 cggcgacgcg tccactcagt gaattaacaa cacagccggt aaaacaaaaa gaggcggaac
    14401 ctacgcctta actttgctgt atatcgagca atgccattat gtgcataaaa ttggcattgc
    14461 tcttttttcg ccacgaaatg ccccccattc tacggtctat gcctttattt tcttatatca
    14521 gagaagattt taattttatt ttatcaattt gttaatcagt acacctctat ttatattttt
    14581 cgccactccg ccattctccc tgacaatttt cttgtggaac aaagtgttat ttaagcaaca
    14641 agcaaattat tctgtctctt tagacaaatt ttgacatcaa acatgatttt tttcgatata
    14701 tatgtaaata tatagcggtt ttaattatca ggatataact tatgtcacta accagacgga
    14761 ggtttacaca gattcttgcg tcgacgttgt tcctgcatca tctgccttct tttgcacaat
    14821 cagtcaaatt ctgggcctca ctgacgcttc ccgaagccca aaacattacc cgcatcgtca
    14881 gcgcaggtgc gcccgccgat ttgctattgc tggctgtcgc gccagaaaaa atggtcggtt
    14941 tttcctcttt tgattttgcc cgtcaggcat taattccatt gccagagcac attcgccagt
    15001 tacccaggct gggacgactc gccgggcgcg ccagcacact ctcgcaggaa gggcttatgg
    15061 cgttacatcc cgatttggtc gttgattgcg gcaatacgga tgaaacctgg atctcccagg
    15121 cacggcaggt tagcgaacag acacaaatac cctggttatt gcttaacggg aaactggagc
    15181 aatcagcaga acagttaaca acgcttggca aaacgttagg cgaagagcat cgcgccgcag
    15241 aacaagccaa tctcgccagc cgcttcgttg gtgaagctca ggcattcgcc acctcacccg
    15301 ccgctaacct ccgcttttat gctgcgcgcg gtcctcgagg gctggaaacg ggcttacagg
    15361 gttcgttgca taccgaggcg gcggaattat taggtttgca caacgtcgcg caaatagccg
    15421 atcgccacgg cctgacacag gtttccatgg aaaatctcct gcgctggcag ccggatatta
    15481 ttctggttca ggaggccgtt actgcagact ttattcgtcg tgatccactc tggcagggcg
    15541 tgaaagccgt tgcggaacaa cgcatcttat ttttaagtgg cttgcccttt ggctggctgg
    15601 atgccccacc gggaatcaac cgtcttctgg gattacgcag acttcacgcc tggctggatc
    15661 ccgccatcaa tcgccagttt aaaagtgaca tgcagcatta cgcccaactg ttctggcatt
    15721 gttcactcag tgacgccgac tatcaaaaat tggtggcgag ctaatgagaa tcgtaaacgg
    15781 atgtattttg ctgctggcag ctatcagtat cacttttgcc gccgtaagtg gggcttatca
    15841 tctcgatatg cagcaactgc tggcgctaat tcttcgtcaa gaaaatgttc ctgtgcagga
    15901 acaaattgtc ttctggcaaa tccgcgtgcc gcgtattctt gctgcgctgt tactgggcgc
    15961 ggcgctggca ggagccggaa ccacctatca gggaatgttg cgtaatccct tagtttcacc
    16021 ggatatttta ggcgtctccg ccggtgccgg acttggtgcc tgcacggcaa ttctgtgggg
    16081 attatccatt gtttacatcc agctgtacgc attttgcggc gggctgatgg tggtcgcggg
    16141 cgtctggttg attacgcgtc gggtgacccg ccacgatccg atcttaaccc tggtgctggt
    16201 aggcattgcg ttaggaacgt tgtgcggagc ggggatatcg ctaattaaaa cgctggccga
    16261 tccttacacc cagttaccgt caattacctt ctggttgctg gggggactgt ccaccgtgac
    16321 cttacgcgat ctctgctatg ctgcaccgat cattctcata ggctccttgc ccctgttttt
    16381 tctccgctgg cgcatgaatt tactcacgct ttccgacgac gaagcccgtt cgctgggact
    16441 gaacgtgacg cgtctgcgct ttggattgat tgtctgcgcc acactaatca ccgccagtac
    16501 ggtagcaata gcgggcataa ttggttgggt cggcctggtc gttccgcaca ttgcccgctt
    16561 gttgaccggt cataaccatc agcagctgtt gcccatggca atgtgtactg gcgcgattct
    16621 gctcctgctt accgatacgc tggcacgcag cattggaaca acagaaatcc cacttggcat
    16681 tctgaccgct tttgttggcg caccgttttt cctgcttttg ttgttacgcg gaggtcgcca
    16741 gtgactttgc ttgccgtccg tcatgcgtct ctgggttact cccgccatcc ggtgctacgc
    16801 gacgtgtcct tcaccctgtc gcaaggaaca atttgttgtc tgttaggagc caatggttgc
    16861 ggcaaaacca cactcatgcg ctccattctt ggcgttatac cgttattaaa aggcgaggtc
    16921 ttactggata gcctccctgt gcagacactc agccatcgcc agcgcgcgca ggctattgcc
    16981 tgggtgccgc aggcgcacga cggtattttt gcttttagtg tgctggatat ggtgttgatg
    17041 ggcctggccc ccaccatagg cgctttttcc gttcccggta agcaagaacg cctgaaagcc
    17101 acggagcaac tggaaaagct gggcattttg catctggcgg cacgacgctg gaatacgctt
    17161 agcggcggtg aacgtcagct ggctttaatc gcccgtgcac tcgtccagca accgcgcttg
    17221 ttgttactgg atgagcctgc ctccagcctc gatttcggtc atcagatcca actactggac
    17281 acgctggcac agctcaaaaa taacggcatg acaatgttaa tgtcgactca tcatccgctc
    17341 catgccaacg ccattgccga cagcattatc caggtagagc cagatggacg agtaacacaa
    17401 ggcttaccga ccgaacagtt aacaacaaac aaactggctg ccctgtatcg ggtttctgcc
    17461 gaccagattc atcatcacct ctctgcaatt agccactaag gaacaatcat gctcattgat
    17521 gatattgatt ttgcagacct ctacctgcag caactcaaac tggcccaccg cacagaaaaa
    17581 acgccggatc actgggatca acgggcggaa aaaatggcgg aaaattgcgc ctcgcctacg
    17641 gacagctacc tgcaacaact gatctcaaaa attgacctac aaggcgctca gaccttattc
    17701 gatatgggct gcggaccggg taccgtatcg ctggcgctgg ctgacaaact cacgacgatt
    17761 tatggcgttg attacagtca ggggatgctg aacgttgccg cccgtcgcgc cgccgcgctg
    17821 aaggcagaca atgtccactg gatccagcgc gcctgggaag aggactggag tgacctgcca
    17881 cgctgcgata ttgctgtggc ttcccgctcc actctggtgg ctgatatgcg ccaggcaatg
    17941 agcaaactga ataatcaggc acggcttcgc gtctatacga ctcatctggt cagcacctcg
    18001 ttcgtctctc cagccattca gcgggcggct ggtcgggaag tgatcgaatt acccaactat
    18061 atctttgcgc tcaacgtcct gtatcagatg ggaatttatg cccacgttga ttttatccgt
    18121 ggacaaaatt gccaacagga taacagcacc tgggaacgtt ttgagcagaa cgtcagctgg
    18181 agtcttggcg cgcttaacga cgatgagcgt gagcggttat atcgctggta tcaacaacaa
    18241 gatgctcgtg ccctcgcccc tgccagccgc gactgggcgc tcatctggtg ggatagcgta
    18301 ccgcaggagg cactgcgatg attttccttt ctcaggcaca aatcgatgcg ttacttctgg
    18361 aagatatcca gggcggtgac ctgaccacga gagcattaaa tattggacac cagcatggct
    18421 atatagagtt ttttctccgt cagggcggct gcgtcagcgg cgtttctgtc gcgtgtaaga
    18481 tgttaactac gctgggatta actattgatg acgcggtcag cgacggttca caagcgaacg
    18541 cgggtcagcg gctaatccgt gcgcagggta atgccgcagc acttcatcag ggatggaagg
    18601 caatccagaa tgtgctggag tggagttgcg gtgtttctga ttatctcgat caaatgctgg
    18661 cgttacttcg tgaacgttat cctgatggca atatcgcctg cacccgaaaa gcaattccgg
    18721 gcacccgctt actggcctcg caggcaattc tggctgccgg aggactgatt catcgtgccg
    18781 gatgtgcgga aaccatatta ttgtttgcta accaccgcca ttttcttcat gacaatcagg
    18841 actggtcagg cgcaatcaat cagttacgtc gccacgcacc cgagaagaaa attgttgttg
    18901 aagccgacac gccgaaagag gcaatcgccg cgttacgcgc gcaaccagac gtacttcagc
    18961 tcgacaaatt tagtccgcaa caggcaactg aaattgcgca aatagcgccg tcgctggctc
    19021 cccactgcac gctggctctg accggcggaa ttaatctgac gacactcaaa aattacctcg
    19081 actgcggcat tcgcctcttt attacctccg cgccttatta cgcggcaccg gctgacatca
    19141 aagttagtct gcaacccgca gcctctattt aattctttgt taaacggaat ttattctatg
    19201 agactcaaaa aacattatct ctgcacagca ttatcacttg cctttaccca gcaggccgta
    19261 gcggttcagg agagcgactc gctgaccgta tggtccagtc cggtatcatc gacgacgacc
    19321 accgttctcg atcaacccac catgaaggcc ctggataaac agaatgtcgc tcaggcatta
    19381 agtgtcgtcc ccggcgtggt gctgcaaaag tcaggcagcc gcaacgaaga acaggtaaaa
    19441 gttcgtggct ttgatagtcg gcaggtgccg gtctatttcg acggtgtgcc catttatgtt
    19501 ccctatgacg gcaacctcga tctggcgcgg attctgacca acaatctggg ggcagtcgaa
    19561 gtttccaaag ggtattcgtc gctgcttcag gggcctaatc agatgggcgg agccattaat
    19621 gtcaccaccc agaagccaac gaaacctctg gaagcaagtc tgggatatcg ccagggatgg
    19681 agccgtagcc aggacaatgc ctacgatatg catgcttcat ttgccgccag cagcgaactg
    19741 gggtatttgc aagtcagcgg tagtcagcta aagcaggatt ttctcggcct gccgcatggt
    19801 gtaaataatg atattgcagg caaacacggc aagatgatta attcatcggc tgatgataaa
    19861 cgcggcattg tgaagctcgg atttacacca cgtgaaaacg atgaatacac actgacttac
    19921 attaagcagg atggtgaaaa agataacccg ccatacagcg gaaatagtgg tcaaaaatca
    19981 cgctactggc agtggccaga gtatgacaaa gaaagttttt attatcaggg aacgacccaa
    20041 ctaaacgatc gttttaccct gaaaagtcgg ctgtatcgcg acacctttga aaatacgctg
    20101 atgatgtaca actcgctggc tgatttgaaa aataaaaaag gcagctacag ccattattcc
    20161 gactacagcg acggtgccgg attacaactg gcagccgatg tgcgtgaaaa cgatctgctg
    20221 tcgtttgccg ttaactggaa agatgacgta caccgggaaa aaggtgcgcc gcacgccgct
    20281 tacgatcgct atgaagatcg tacctggtcg ctcgccagtg aatatcaatg ggctgctgcc
    20341 gataatgtcg atgttgtggc tggaatcagc tatgactggc gcgatagtgt agaagcgaaa
    20401 aaacacgaga aagatggcag catcacccat tatgacgaca acaatcagtc agcttttaac
    20461 tggcaggtga tggggaaata ccactttgtc aatgaagaca cgctggcgct ttcgtactat
    20521 gaccgcacac gctttccgac gctgaaagaa cgctatacca cgtccaaacc tgcgtataac
    20581 cagatagcga ttgttaaccc gcagctcaag ccggaacgcg ctcgcggggt ggatttaacc
    20641 tggaatggtg ccttcacgcg cgactgggga tttgaggtca gcgtttacta taaccgggtg
    20701 agtgatgcca tcctctcgca caatatcgat gccgatacca ttcaaaatca gaacagcggc
    20761 acggtggatt acagcggtct ggatgccggt attaagggga aaatcagcaa tatactggat
    20821 gtaggattga gctacgccct gatccacgct gacgccaaac gcaaagacat cggcaagata
    20881 accgatctgc caacgcagac aatgaccgca tggatgactc tcaaaccgtg ggagccgtta
    20941 agcgtaacgc tgtcggaaga ggcgcgttcc tccagctaca gcaacagtga cggttcacaa
    21001 aaagccgccg gttttgcggt gacccacatt cgagccgatt acaccttagg tcatggcttc
    21061 agcgtcaatg cgtcggtcaa taacctgttt gatacccaat atgcctacag tgaagggttt
    21121 attgaagaag gccgcaattt ctgggcaggt gtcgagtata cgttctgatg tgaaagcgga
    21181 tagcggcatc tgtatcgcta tccgccttat gaaaatctta ttctcttcgc aggaggcgga
    21241 atacgcccag aacgagaata gctcccacca ccgccaccag gaagctgtgc aaattaaaac
    21301 cgctgatgga gccgccaatg ccaaacatgg tcgccagcca gccgccgacc accgcaccga
    21361 ctatcccgag aatacaggtc aggaaaaatc caccaccatc acgccccggc atgattagct
    21421 tggcgataat gccggctatc aggccaaaaa taatccaggc aataattccc atgttcaggc
    21481 cctcgtttcg atgaatcgga cgcaagaaat gcttacgtca acgctctaag tataggcaag
    21541 ggcgggatcg atgctgggcg tttgtcggct gatttaagaa caacatgtaa aaaatattca
    21601 cgcatttgta ttaagttttg ttaactatga ccgataaacc aaagacagtt tgtcagtcag
    21661 gagtttttcc gcgtgagtca ttaccatgag cagttcctga aacaaaatcc gttagccgtc
    21721 ctgggcgtgt tacgcgattt gcacaaagcc gcaattcctt tgcgtatcag ctggaatggc
    21781 gggcagctga tcagcaaaat actggtaata accccggata aactggtgct ggatttcggc
    21841 agtcaagccg aagacaacat cgccgtacta aaggcacggc acattaccat taccgccgaa
    21901 actcagggtg cgaaagtcga gtttactgtt gaacaactac aacagagtga atacttgcag
    21961 cttccggcat ttattagcgt accgcctccc accttatggt ttgtacaacg acgccgatat
    22021 ttccgcatct ccgccccact ccatccgcct tatttttgcc agaccaaact ggcggataac
    22081 agtacgttac gtttccgcct gtatgatttg tcgttaggcg gcatgggcgc attactggaa
    22141 acagcaaagc ctgccgggtt gcacgaaggt atgcgcttcg ctcagattga agtcaacatg
    22201 gggcaatggg gtgtttttca ctttgacgcc cagttaatct ccatcagcga gcgcaaagtg
    22261 attgatggca agaatgaaac catcaccact ccccgtctga gcttccgttt tcttaacgtc
    22321 agcccgacgg tggagcggca attacagcgg attattttct ctctcgagcg ggaagcccgg
    22381 gagaaagcgg acaaagtacg cgactgattt acatcgcgtc cagtgcctgc tcaagtttgt
    22441 agatatagcg cggagcctgc ggcgcaggat gatttcgcgc tacgtgatcg aggaactcgt
    22501 cagcatccag atcgttgatt ttgctgattg cctttttccg atctgacgag aaagtccgta
    22561 gcagcgcacc tgccccgtta gcgtatgaca ccaccagcgc atattgcagt accttcggat
    22621 cttcaatgcc tgccagcggg ccggtttcca gaatattcag gtaagccgcc cccattgaaa
    22681 tattacgctc aggatttttc agctcgctgg tcgtcggctc accactccag cccatacggc
    22741 gatatacatc acgtccggag gttgaagctt ttaactgcat caaaccaatg gcattcgatt
    22801 tactcaccgc gttcggatta ccacccgatt cgatagcgat aatcgccgtg atcaattgtg
    22861 gatcgacgcc ccaggctgca ccggctttct ggcttattgg catccactgc atcgcacgtt
    22921 gcaccggaac tttcgcgttc cacggcgggt tcgtgtagtc atgctttgat gaacaacccg
    22981 ctaataacac aatcaaaaag gcaaaccatc tcaatttcac ttcttctatc cttatagcct
    23041 ggaatcgccg ttgaggtgac aacggcacac ctaagcggca tgatatacat ttcgtttctg
    23101 ttgtcagcaa ggaattgcca tgtctctgtt tcacttaatt gccccatcgg gttactgcat
    23161 taaacagcac gccgcgctgc gtggtatcca gcgccttacc gacgcggggc atcaagtcaa
    23221 taacgtcgag gttattgccc gtcgttgtga gcgttttgcg ggtacggaaa cggagcgtct
    23281 ggaagatctc aattcccttg ccagactgac cacccccaac accatcgtgc tggctgtacg
    23341 cggcggttac ggtgccagtc gtttactggc agatattgac tggcaggcac tggtggctcg
    23401 ccaacaatat gacccgttgc tcatatgcgg gcatagcgat tttaccgcca ttcagtgcgg
    23461 tcttctggcg caaggcaatg tcatcacctt tagcggcccc atgctggtag caaactttgg
    23521 cgcggatgaa ctgaacacct ttaccgagca ccatttctgg ctggcgttac gcaatgaaac
    23581 ctttaccatt gaatggcaag gggaaggccc gatatgtcgg gccgaaggca cgttgtgggg
    23641 aggcaatctt gcgatgctga tttcactgat cggtacaccg tggatgccaa aaattgagaa
    23701 cggtattctg gtgctggaag atattaacga gcaccctttc cgcgtcgaac gtatgctgtt
    23761 gcagctctat catgctggaa ttttgccacg tcagaaggcg attattctcg gtagctttag
    23821 cggcagtacg cctaatgatt atgacgcagg ttacaacctg gagtcagtgt acgcgtttct
    23881 gcgttcccgc ctgtcgattc cactcattac cggcctcgat tttggtcatg aacaacgcac
    23941 ggttacgctg ccgttaggcg cacatgccat gcttaataac acccatgaag gcactcagtt
    24001 gacgattagc ggtcatcctg ttcttaaaat gtaaaaaaat cacgcttcag ggctaagtaa
    24061 aacgctgtct ctgccgctaa tatggtaggg tttatataag agacagcgta atcaggagta
    24121 accgaccgtt ggatgccaca acaataatta gccttttcat cttaggatcc atccttgtca
    24181 ccagcagtat attacttagt tcattttctt cccgtcttgg cattcctatt ctggttattt
    24241 ttctggcgat cggcatgctg gcgggagtcg atggcgtcgg cggtatcccg tttgataatt
    24301 accccttcgc ctacatggta agtaacctgg cgctggcgat tattttgctc gacggcggga
    24361 tgcgcactca ggccagttcc tttcgtgtgg cgttaggacc ggcgctgtcg ctggcgacgc
    24421 tgggcgtact tatcacctct ggtttaaccg gcatgatggc ggcgtggctg tttaatcttg
    24481 atttgattga aggcttatta atcggcgcta tcgtcggctc caccgatgct gcagcggtct
    24541 tttctctgct gggtggtaag gggcttaacg aacgtgttgg ctcgacgctg gaaattgaat
    24601 ccggcagtaa tgatccaatg gcggtctttc tgacgattac cctgattgcg atgatccagc
    24661 agcatgaaag cagtgtcagc tggatgttcg tggttgatat tctgcagcaa tttggcctcg
    24721 ggattgtcat cggtcttggc ggtggttatt tactgctgca aatgattaat cgcatcgccc
    24781 tgcccgccgg tttatatcca ttgctggcgt taagcggcgg tattttaatt tttgctttaa
    24841 ccacggcgct ggaaggtagc ggtattctgg ctgtttatct gtgcggtttt ctgctgggta
    24901 atcggccgat tcgcaaccgc tacggcatcc tgcaaaattt cgacggcctc gcctggctgg
    24961 cgcaaatcgc catgttcctg gtgctggggc tgttggttaa cccaagcgat ctgctgccta
    25021 ttgccattcc ggcgctcatt ttgtccgcat ggatgatttt cttcgcccgt cctctttcgg
    25081 tatttgccgg attgctacca ttccgtggct tcaatctgcg tgagcgcgtg tttatcagct
    25141 gggtgggatt acgcggcgcg gtgccgatta ttctggccgt gttcccgatg atggcggggc
    25201 tggagaatgc acgtctgttc tttaatgtcg ccttctttgt ggttctggtt tcactgctct
    25261 tgcagggaac atcactctcg tgggcggcga aaaaagccaa agtggtggtt ccgcccgtcg
    25321 gacgtccggt gtcacgcgtt ggcctggata ttcatccgga aaatccgtgg gagcagtttg
    25381 tttatcaatt gagtgccgat aaatggtgcg tgggcgcggc actgcgcgat ttgcatatgc
    25441 caaaagagac gcgtattgca gccctgtttc gtgataacca gttgcttcat cctaccggca
    25501 gcacccgact gcgcgaaggc gatgtattgt gtgtaattgg tcgggaacgc gatctcccgg
    25561 cgctcggtaa actgttcagc cagtcgccac cggtcgcgct ggatcaacgc ttctttggtg
    25621 acttcattct cgaagccagc gccaaatatg ctgatgttgc gctgatatat ggcctggaag
    25681 acgggcgaga atatcgtgat aaacagcaaa cgctgggtga aattgtccag cagttgttag
    25741 gcgcggcacc ggttgtcggt gaccaggtag agtttgccgg aatgatctgg acggtggccg
    25801 agaaagaaga taatgaagtg ttgaagattg gcgttcgagt ggctgaagag gaagctgaat
    25861 cttaaattta atgaagcatc tatcgccggt tacgatgctt tgcttaacgc agcctgagcc
    25921 tgtatataaa cttgtcattt cctgattttg atatgcacat tccaacatca gaagaaggtt
    25981 aaattatgga tgacaaacca ttgcaggctc tggccaataa actcaaaaca ccggctcaag
    26041 aaagtcagcg taatggcttc ttccgactgc aacaggtgaa gaataaccag ccccgcatta
    26101 ccggtatccc cgtcaaccat caggggcccc agcctgacat cgcctgaaca tattccccca
    26161 cgcaacctgc cgcactgaca tagcccacac aataggcgtt tcatccgcct gtagcaccgg
    26221 atgcatgagc gatttcttat agttattcct ttgcttaata acgattattt ccgggtcgtt
    26281 cgctctcgtc ttttctttaa tacgtcagag ctacctgggc cgtaatacag catgtaccag
    26341 acccgacacg gcatttctga ccagggaaaa agtcaacgat gagtagttat atcagcgcaa
    26401 atgacgtcct gtggctcctt tgcggtgacc acattcacgt ctccggcgct gccagaggat
    26461 atttatttca aggatacgta gcattatggc tagctttaaa gacatctgcc cattcgcccc
    26521 cgccattccc ccatcttccg ttaacacagg cgtacgacgc ccgccagggc ggctcttcct
    26581 gtcggtggaa ctcaagaatc aaattttgca gaacatgaag tatctcagcc cgcaaaaatt
    26641 cagctggggt gatgccccct ggcagattgt cgatctcagt atcgcgggca aagtgaacat
    26701 acaggtggat aacaatacca tcatcacgtt ggggactccc ctgaatcagc aacataacga
    26761 gttcatgatg gtcgccaaat ggtgcgaatg ggctatacag caggatggtt tgcaagaaaa
    26821 cctgcaaaaa aaactgcacg aggtcctgga agaaaaccgg caaaacaaac agtcagaaat
    26881 tccccaagaa gacctgaaag aaaggctgaa agaaatcaag gaagatatcc tgaaagaaaa
    26941 ccagtcagca agccagattg aagaccgggc agaagcattg cgcagaatga aggaatgtct
    27001 cataacaaga cagagtatgc tcgatcttag caaccttgga ctgacttcac tccctgaaaa
    27061 tttgcctcca catctgattg aatttaactg cagtagaaac gtgttgaccg cgttaccgga
    27121 ggtaatgcca aaggggctga gagtgcttga atgtatggag aactttttga tcttgttacc
    27181 gaaggtgcag cccccgaaac tgatggtact gaagtgctat gaaaactata ttatctggct
    27241 gcctgagctg tcgactaacc tgagagtgat taactgttct gaaaacttct tgcaattttt
    27301 accgccgtcg atgccccagt acctgtataa actgtcctgt gctggcaaca atattaactc
    27361 aatacctgat gagatgctgg agaacttgac tcgcctgaag gtattcgact gttctagtaa
    27421 cgatttgatc tcttcaccac ggctgccgcc caaactaatc atatactact gtggagaaaa
    27481 tcagtttaaa actgtacggg tgccgcagcc ccagagcctg aaggtgtttg actgtaatgg
    27541 taacccttgg gacaaagaca atttaccgac gctgctcaaa gccgtcgagg gcctgaaaaa
    27601 ccagcagggc ctggaggacc ttttagactt tttgcgcaag gaaggccaga ttgacctgga
    27661 gggactggag gagctggagg acctggatga ccttatggat ctggagttcc tggatgaccc
    27721 ggaactcctg gagcgcgtga aggtacagga ggacctggaa ctcctgaatc agcagttggg
    27781 cctgttgagt ctggaaagac aacaggactc gcagcctgtt aatcaacaat ctgaacatga
    27841 acccgaacct gcatcaaggg tgaagcgtga tttatctgaa gtcgagttcg agtcaacaat
    27901 gaaacgtaag cgttttatgt agaacgcctg tgcggtgagt tcaggttccg gggaaatagt
    27961 gtccatcatt ttaatgggca ctatcatggg caatggctct gtcacattaa caactacagt
    28021 tgctgaccag ccggatggat gatttttttt gcaaccagaa gacgttgcct ccgatccggc
    28081 ttacaacaag ttacaccgtc acaaccggga cacgtaacgc cagcgcgcac atcaactcat
    28141 agcccaccgt tccggcagcg gcggcgacat catcaatttt gatctccttg ccccacagct
    28201 caaccggcgt accaataccc gcctgcgggc aaggcgttaa atcgaccgcc agcatatcca
    28261 tcgaaactgt ccctactgtc atggtgcgca cgccgtcaac taaaacaggg gtgccggtag
    28321 gcgcatggcg cggataaccg tcggcgtatc ctgcggcgac aatgccgatt cgctgttcat
    28381 ctcgcgcagt atagcgaccg ccgtagccca cacgctcgcc cgcttttagc gtctggacac
    28441 caataatctc actgcttagc gtcatcaccg gacgtaatcc ggtattggcg atatcacgcc
    28501 actgaccgga cggcgaagcg ccatacaaaa taatgccagg ccgaacccag tcaaaatgcg
    28561 cttccgggtg ccacagagtc gccgccgaat tggacaacga acgccgacac tccagccctt
    28621 ccgccgcctg ctcaatacgc gccatcgcgc cggaaattcc atcaggatgt tccgcctcgg
    28681 caaaatgcga catcagggtc atttcgccaa cattcgccat tgcccgcaac tgctgccaga
    28741 cggtaagcac gcgatcgggc tggaagccca accgattcat cccactgttc actttaagat
    28801 aaatatccaa cggtgctttt agccgcgcat tttgcagtgc tttgagctgc cagttgctgt
    28861 gtacgcaggt ggtcaggcgg tgctggtcat aaatctccag atcctgagca tggaaaaatc
    28921 cttccagcat caggatcggt cctttccagc cgcgctcacg taacgttatt gcctcttcca
    28981 ggttaagtaa tgcaaagcca tcggtagccc cgagcgcgct ccagatacgc tcaataccgt
    29041 gcccgtaagc gttcgctttt accaccgacc agacgcgcgc gtgcggcgcg gcctggcgga
    29101 caatggacag attctgtttt aatgcctgca gatcgaggct ggcctgtatc ggacgggtca
    29161 tctcgtttcc ttagctgtgt gcgccatgta aatggcccgg acgtgatggc gtaaatccac
    29221 ggctgtagcg cgctacgctt agatcctcat atgggatcgc tggtgtgcga ccagagagca
    29281 ggtcgcttaa caactgaccg gaaccgcaag ccatcgtcca gccgagcgtg ccgtgaccgg
    29341 tattcagcca cagattttta aagcgtgtac gcccgacaac cggcgtgccg tctggcgtca
    29401 tcgggcgcag acccgtccag aaagtcgcct gctcgacatg accgccgcgt ggatagagat
    29461 cgcgaaccac catttctagc gtttcacgac gcggttgcaa cagctcggtg ttaaaaccaa
    29521 caatttccgc catcccgcca acacgaatgc ggttatcgaa acgggtaatg gcgattttgt
    29581 aggtttcatc aagaatggtg gatactggcg caccatcttc ctgcgcaatt ggaatagtca
    29641 gcgagtagcc tttcagcgga tacaccggaa tatcaacaat gcctttgagc atcgccgtcg
    29701 agtaagaacc aaacgccatc acatacgcat cggccttaat cacttcatcg ccacacttca
    29761 cgccgtagat ttgctcgccg tcgcaaagca gttggtcaac gggcgtatta aagcggaatt
    29821 taacccccgc ctgctccgcc atccgcgcca gattctgggt aaatagctga cagtctccgg
    29881 tttcatcatt gggtaactgc aggccgcccg tcagtttgtg cgccacttct gccagcgcgg
    29941 gctccacttc cgccaggcgg ctggattcca gcagctgata cggtacgccg gcatcttcca
    30001 gcacggcgat atcgcgggtc gcattttcat actgttgttc ggtacggaac agttgcagcg
    30061 tcccaccctg acgcccttca tactgaatat tggtttcggc gcgcaatgct ttcaggcaat
    30121 cacggctgta ttccgccaga cgcaccatcc gccctttgtt ttccatgtag tggctggtgt
    30181 cgcagttacg taacatttgc cacatccatt tcaactggaa ctgcgtaccg tcgagacgaa
    30241 ctgccagcgg cgcatggcgc tggaacatcc atttgatcgc ttttaaaggc acacctggtg
    30301 ccgcccacgg tgcagcatat ccgggggaga tttgcccggc attcgcggca ctggtctcca
    30361 gggcggcccc cggctcccga tcaatgacgg tgacctcatg tcctgcctga tttaagtacc
    30421 aggcgctggc aacgcctacc acaccacttc ccagtatgac aactcgcata gccactccga
    30481 taacggtaga tacagtaaaa gaataataat ctaattacat cttgataact cagttgaaaa
    30541 tattattcaa catacggctt ttttatggtg agctggctca catctccctg actctgcggc
    30601 aatatggcgt ctttagtatc tcctgctgaa cgcccttttt atccagcata aaattccgtt
    30661 cagaagcgga ttagtggcac tctgataata agaaatcgca aagaaaaaaa ttctccggcg
    30721 acgcggtttt taacaggtgt tctatgcttg aaatgaggtg ctccacacag agagcgccga
    30781 caacaatgag gtgcgcgtat ggcgacgatc gattctatga ataaggacac cacacgtttg
    30841 agcgatggac ccgactggac gttcgacctg ctggatgttt atctggcaga gatagaccgg
    30901 gtggcgaaac tctaccggct ggatacctac ccgcaccaga ttgaagtgat aacctcagaa
    30961 cagatgatgg atgcctactc cagcgtcggc atgccaatta actatccgca ctggtcattc
    31021 ggtaaaaagt ttatcgagac tgaacggctg tataagcacg gtcagcaagg actggcctat
    31081 gaaatcgtca ttaactctaa cccgtgtatc gcttacctga tggaagagaa caccattacc
    31141 atgcaagcgc tggtgatggc tcatgcctgc tatgggcata actctttctt caaaaacaat
    31201 tacttattcc gtagctggac cgacgccagt tcgattgtcg attatctgat ttttgcccgt
    31261 aaatatatta ccgagtgcga agagcgctat ggcgttgatg aagtagaacg gcttctggac
    31321 tcgtgccacg cgctgatgaa ctacggcgtg gaccggtaca aacgcccgca aaaaatctcg
    31381 ctgcaagaag agaaagcccg gcagaaaagt cgcgaagagt atctgcaaag tcaggtcaat
    31441 atgctctggc gtaccctgcc gaagcgcgag gaagagaaaa cggttgctga agcgcgccgc
    31501 tatccgtccg agccacaaga aaacctgctc tattttatgg agaaaaatgc gccgctgctg
    31561 gaatcatggc agcgtgaaat cctgcgtatt gtgcgtaagg tgagccagta tttttatccg
    31621 caaaaacaga ctcaggtgat gaacgaaggc tgggccacct tctggcacta caccatcctt
    31681 aaccatctgt atgatgaagg gaaagtaacg gaacgtttta tgctggagtt tttgcacagc
    31741 cacaccaatg tggtcttcca gccgccctat aacagcccgt ggtacagcgg catcaacccg
    31801 tatgccctcg ggttcgccat gttccaggat attaaacgga tttgtcagtc gccaacggaa
    31861 gaagacaaat actggttccc ggatatcgcc ggttccgact ggctggaaac gctgcatttc
    31921 gcgatgcgtg atttcaaaga tgagagtttt atcagccagt tcctgtcacc gaaagtgatg
    31981 cgtgatttcc gcttcttcac cgtgctggat gatgatcggc ataattatct ggagatttcc
    32041 gctattcata atgaagaggg ttatcgggag atccgtaacc ggttatcgtc gcaatataac
    32101 ttaagtaatc tggagccgaa tattcagatc tggaacgtgg atttgcgcgg cgaccgttcg
    32161 ctgacactgc gttacattcc acataatcgc gcaccgctgg atcgggggcg caaagaagta
    32221 ctgaagcatg tgcatcgcct gtggggattt gatgtgatgc tggaacagca aaacgaagac
    32281 ggcagcgtcg agttgctgga acgttgcccg ccgagaatgg gaaatctgta aaaacaacaa
    32341 aaaacccctc gtttgagggg tttgtttttt aaacggaagg gattatcgcc cctgaatggc
    32401 taaatcaccc ggcagatttt tctgcatccg gtgccaaatc tcgccactct catgcccata
    32461 gcgacgcact gtttcgtaca cctgatcgtg cgcgccttca ctgcacaacg ccgacagttt
    32521 gtggtagaag cccagcgcca gactgcgcgc ttccggattg gcgaaatagt gacgaccaat
    32581 gcgcgtatac agtcccttca tcccgttaag gatcagaccg taaatcgggt tgccggaagc
    32641 aaacgccagg ccgcggaata tgttgtaatc cagctcggca aaggcatcgg cgtgatcggc
    32701 cacttcatta gcggtagcca gcacttcctg cgctttatcg ggatgctgac gaaacgcggt
    32761 gcgaataaaa atagttgaaa tattggtacg caccgacagc aaattatcaa taagctgcgg
    32821 cacactttcg tgatccagtc gcgccagtgt ttcaaggata tttaaaccag aggtttccca
    32881 gaaattattc accttcgtcg gcttgccatg ttgaatggtc aaccagccat ctcgtgccag
    32941 acgctgtaac acttcacgta acgtagtacg cgttacgcca attaattctg aaagttcacg
    33001 ttctgcgggc aaaatagtcc cgggagggaa gcggttattc cagatacttt caataatgta
    33061 ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc atagtgagat ttccataaca
    33121 cagcaaaaca aagttggact catcatacca gagggctaac tacgctgata gcagacaaaa
    33181 agaagaaaaa agggatcaag gcttcatttt ccgcatctct ttccccttga ctatcaagac
    33241 ttgcctcact cctgcctgac cgttaagctt tgcgtttaaa cataaaaaca tcattgatta
    33301 tttgtaaggt aagggaaacc atcatggaga tctcctgggg ccgcgcgcta tggcgcaatt
    33361 ttttgggcca gtcccccgac tggtacaaac tcgccctcat cattttctta atcgtaaatc
    33421 cgttaatttt cctcatcagc cctttcgtcg ctggctggtt gctggtagcg gaatttattt
    33481 tcactctggc gatggccctg aaatgctacc cgctactccc cggtggtctg ttggctatcg
    33541 aagcggtatt catcggcatg accagcgcgg aacacgtccg tgaagaggtg gcggcaaatc
    33601 ttgaagtctt gctgttattg atgtttatgg tggcgggtat ctattttatg aaacagctgt
    33661 tgctgttcat atttacccgt ttgctgttaa gcattcgctc caaaatgctg ctgtcgctct
    33721 ctttttgcgt ggcggcagca ttcctctccg cgttcctcga tgccttaacc gtcgtggcgg
    33781 tggtgatcag cgttgcagtc ggtttttatg gtatttatca tcgcgtagcc tcttcccgta
    33841 ccgaagacac cgacctgcaa gacgatagtc atatcgacaa gcattacaaa gtagttttgg
    33901 aacagttccg tggctttctg cgtagcctga tgatgcatgc cggtgtcggc accgcattag
    33961 gcggcgtgat gaccatggtg ggcgaaccac agaacctgat catcgctaaa gcggctggct
    34021 ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac cgttccggtt ctgatttgtg
    34081 gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt tggctacggt gaaacgctgc
    34141 cggagaaagt ccgcgaagtg ctgcaacagt ttgacgatca aagccgccac cagcgtaccc
    34201 gtcaggataa aatccgtctg attgtccagg cgattattgg cgtctggctg gtgactgcgc
    34261 tggcgttgca tctggcggaa gttggcttga ttggtttgtc agtcattatt ctggcaacat
    34321 cattgaccgg tgtcaccgat gaacatgcta tcggtaaagc cttcaccgaa tctctgccat
    34381 tcaccgcact gttgacggtg tttttctcgg tagtcgcggt gattatcgac caacaactgt
    34441 tttcgccgat tattcagttt gtgttgcagg catcggaaca tgctcagctg tcgttgttct
    34501 atattttcaa cggactgctg tcatccattt cggataacgt cttcgtgggg acgatttata
    34561 tcaacgaagc gaaagcggca atggaaagtg gcgctatcac gttgaagcaa tacgagctgc
    34621 tggcggtcgc cattaatacc ggtaccaatc tgccctctgt cgctacgccg aacggtcagg
    34681 ctgcgttcct gttcctgctg acctctgcac tcgcgccatt gattcgcctc tcttatggcc
    34741 gcatggtgtg gatggccctg ccttacaccc tcgtcctgac actcgtcgga ctgctctgcg
    34801 tcgagtttac gcttgcccct gtaaccgaat ggtttatgca aatgggctgg atagcaacgc
    34861 tttgataaca acttaccggg catataaatg cccggtttgc cttttcgccc gataattgtc
    34921 caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg aatgaattgg tttaaactgc
    34981 gcactctatg catattgcag ggaaatgatt atgttgcgat ttttgaacca atgttcacaa
    35041 ggccggggcg cgtggctgtt gatggcgttt actgctctgg cactggaact gacggcgctg
    35101 tggttccagc atgtgatgtt actgaaacct tgcgtgctct gtatttatga acgctgcgca
    35161 ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg ccccaaaaac gccactgcgt
    35221 tatgtagcga tggttatctg gttgtatagt gcgttccgcg gtgtgcagtt aacttacgag
    35281 cacaccatgc ttcagctcta tccttcgccg tttgccacct gtgattttat ggttcgtttc
    35341 ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt ttgtcgcctc tggcgattgc
    35401 gccgagcgtc agtgggattt tttaggtctg gaaatgccgc agtggctgct cggtattttt
    35461 atcgcttacc tgattgtcgc agtgctggtg gtgatttccc agccgtttaa agcgaaaaaa
    35521 cgtgatctgt tcggtcgcta atccattcgg cgctcctgcg ggagcgcttt tttcctgccg
    35581 ctatatttat ttgacccgca gtaaatcaga acttcgcgtt gtataacgtg gtgaaagcat
    35641 ggctcgcttc atctgccatt gttgctggat cccctgcccg gcaaaataga gtgttcctct
    35701 gccctcttta gcattcagtg tatccattac cgtcatcaat tgctcactcc cggggcgcgg
    35761 tgcgttgtca tcgaataaat tgagctgcgc gactccctga ctgaagaaat cccccagcat
    35821 cacgcccgct ttttggtagc gatggcccgc ttgccagatg gcatccagag atcgcgtagc
    35881 ggcgttaatg atatccctgc tgtcctgagt gggcgtcagc agttttaccg acgcgctatt
    35941 gccgtaataa ggttcattga gcgcaaatgg tgacgtctta ataaatgtgg agataaaccg
    36001 acaatattga tgctcactgc gaagtttttc cgccgcccgg gcagcgtaac tacaaatggc
    36061 ctgccgcatc gacgtataat ccgtgatgcg ttcaccaaac gagcgggaac agataatttc
    36121 ctgctttgtc ggtgcaaatt cttccagttg caaacagggt tcaccgcgca gttcacgcac
    36181 cgttctttcg agcacgacat taaaatgttt acggataaac cggatatctg tatccgccaa
    36241 atcgagaacg gttttgatcc ccatcgcgtc cagttttttg ctgatccgcc gtccaatccc
    36301 ccagacgtca tccacgggga gagcagacat taatttacgc tggcgttcca gatttgataa
    36361 atccaccacc ccacccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc
    36421 cagcgttttg gtctgggcga tccccacacc aacagtaaga tgggtacgtt gtagcaccgt
    36481 tgcgcgaatt tctctgccaa agtcagtcag atcgcgacaa ttacgcacac ctgtcagatc
    36541 gcagaatgcc tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga
    36601 catcacccga ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacgcc
    36661 acagcgacga aacagatctt tttgtttgaa ccagggatcg cccattttaa cgccaagcgc
    36721 ctttgcctca gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg
    36781 tttaccccat aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc
    36841 acagagggca aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc
    36901 agcgtatctt cactactgat ggtaatgggc gagtacgcgc tgttcatggg aataagctgt
    36961 accgtcgggc gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca
    37021 atatcaccat ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt
    37081 ccaccatcaa tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg
    37141 atcaacagtt gattcagatc gatgcgctgt tcaacgtaat ctgcggccgg tgaaggaaag
    37201 ccacactgaa caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc
    37261 ttgataaaca acataataat ctgcctgaag ttgtactgtt tttatataca gtagtctgtt
    37321 cttgccagca gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga
    37381 tttttcgttc tcttatcctg tgctcacgcg gccttctggc gacgacgctc atccagcaga
    37441 aatgaaaaat atcacccggc taaaaaatag aatagaagca ttcgccataa tgacattaaa
    37501 cattgtttga tatttatcat attaatataa ataaagacat tgacgcatct ctcccggcta
    37561 actatgaatt agatgaagta aaattcatta a
//
LOCUS       DAZGCQ010000045        36592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000045 DAZGCQ010000000
VERSION     DAZGCQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.045"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1224)
                     /locus_tag="ACHK07_002420"
     CDS             complement(<1..1224)
                     /locus_tag="ACHK07_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8102739.1"
                     /translation="MDYRFSWVIFNMINNISDQVSSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILAINADSDLIKNDVLLAENIPTADYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKTGNERISIGIRNISNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFET
                     KPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVF
                     MSLPVPVATMVREDATNLINLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNE
                     SILKKCCFENGSLGDV"
     gene            1848..2363
                     /locus_tag="ACHK07_002421"
     CDS             1848..2363
                     /locus_tag="ACHK07_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8102740.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            2581..3309
                     /gene="artP"
                     /locus_tag="ACHK07_002422"
     CDS             2581..3309
                     /gene="artP"
                     /locus_tag="ACHK07_002422"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO8102741.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            3327..4058
                     /gene="artI"
                     /locus_tag="ACHK07_002423"
     CDS             3327..4058
                     /gene="artI"
                     /locus_tag="ACHK07_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO8102742.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            4065..4781
                     /gene="artQ"
                     /locus_tag="ACHK07_002424"
     CDS             4065..4781
                     /gene="artQ"
                     /locus_tag="ACHK07_002424"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO8102743.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            4781..5449
                     /gene="artM"
                     /locus_tag="ACHK07_002425"
     CDS             4781..5449
                     /gene="artM"
                     /locus_tag="ACHK07_002425"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO8102744.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            5740..6471
                     /gene="artJ"
                     /locus_tag="ACHK07_002426"
     CDS             5740..6471
                     /gene="artJ"
                     /locus_tag="ACHK07_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO8102745.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(6522..8003)
                     /locus_tag="ACHK07_002427"
     CDS             complement(6522..8003)
                     /locus_tag="ACHK07_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO8102746.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(8015..8335)
                     /locus_tag="ACHK07_002428"
     CDS             complement(8015..8335)
                     /locus_tag="ACHK07_002428"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8102747.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(8357..9700)
                     /locus_tag="ACHK07_002429"
     CDS             complement(8357..9700)
                     /locus_tag="ACHK07_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO8102748.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(9980..11107)
                     /gene="rlmC"
                     /locus_tag="ACHK07_002430"
     CDS             complement(9980..11107)
                     /gene="rlmC"
                     /locus_tag="ACHK07_002430"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO8102749.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(11148..11636)
                     /locus_tag="ACHK07_002431"
     CDS             complement(11148..11636)
                     /locus_tag="ACHK07_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO8102750.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(11696..12541)
                     /gene="potI"
                     /locus_tag="ACHK07_002432"
     CDS             complement(11696..12541)
                     /gene="potI"
                     /locus_tag="ACHK07_002432"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO8102751.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPAERGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(12538..13491)
                     /gene="potH"
                     /locus_tag="ACHK07_002433"
     CDS             complement(12538..13491)
                     /gene="potH"
                     /locus_tag="ACHK07_002433"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO8102752.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMNHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQFSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(13501..14634)
                     /gene="potG"
                     /locus_tag="ACHK07_002434"
     CDS             complement(13501..14634)
                     /gene="potG"
                     /locus_tag="ACHK07_002434"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO8102753.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(14729..15841)
                     /gene="potF"
                     /locus_tag="ACHK07_002435"
     CDS             complement(14729..15841)
                     /gene="potF"
                     /locus_tag="ACHK07_002435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO8102754.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(16192..16668)
                     /locus_tag="ACHK07_002436"
     CDS             complement(16192..16668)
                     /locus_tag="ACHK07_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO8102755.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(16756..17658)
                     /gene="rimK"
                     /locus_tag="ACHK07_002437"
     CDS             complement(16756..17658)
                     /gene="rimK"
                     /locus_tag="ACHK07_002437"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO8102756.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(17719..18441)
                     /gene="nfsA"
                     /locus_tag="ACHK07_002438"
     CDS             complement(17719..18441)
                     /gene="nfsA"
                     /locus_tag="ACHK07_002438"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO8102757.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(18425..18712)
                     /locus_tag="ACHK07_002439"
     CDS             complement(18425..18712)
                     /locus_tag="ACHK07_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO8102758.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18872..19129
                     /gene="grxA"
                     /locus_tag="ACHK07_002440"
     CDS             18872..19129
                     /gene="grxA"
                     /locus_tag="ACHK07_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO8102759.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(19159..19536)
                     /locus_tag="ACHK07_002441"
     CDS             complement(19159..19536)
                     /locus_tag="ACHK07_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO8102760.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            19806..21491
                     /locus_tag="ACHK07_002442"
     CDS             19806..21491
                     /locus_tag="ACHK07_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO8102761.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(21666..22202)
                     /gene="rcdA"
                     /locus_tag="ACHK07_002443"
     CDS             complement(21666..22202)
                     /gene="rcdA"
                     /locus_tag="ACHK07_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO8102762.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVIDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            22286..23494
                     /locus_tag="ACHK07_002444"
     CDS             22286..23494
                     /locus_tag="ACHK07_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8102763.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            23494..24309
                     /gene="ybjI"
                     /locus_tag="ACHK07_002445"
     CDS             23494..24309
                     /gene="ybjI"
                     /locus_tag="ACHK07_002445"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO8102764.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQDIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            24395..24679
                     /gene="ybjH"
                     /locus_tag="ACHK07_002446"
     CDS             24395..24679
                     /gene="ybjH"
                     /locus_tag="ACHK07_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO8102765.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(24720..25952)
                     /gene="mdfA"
                     /locus_tag="ACHK07_002447"
     CDS             complement(24720..25952)
                     /gene="mdfA"
                     /locus_tag="ACHK07_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO8102766.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            26237..26833
                     /gene="ybjG"
                     /locus_tag="ACHK07_002448"
     CDS             26237..26833
                     /gene="ybjG"
                     /locus_tag="ACHK07_002448"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8102767.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQATGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            26891..27649
                     /gene="deoR"
                     /locus_tag="ACHK07_002449"
     CDS             26891..27649
                     /gene="deoR"
                     /locus_tag="ACHK07_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO8102768.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(27696..28898)
                     /gene="dacC"
                     /locus_tag="ACHK07_002450"
     CDS             complement(27696..28898)
                     /gene="dacC"
                     /locus_tag="ACHK07_002450"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8102769.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            29145..29771
                     /gene="gstB"
                     /locus_tag="ACHK07_002451"
     CDS             29145..29771
                     /gene="gstB"
                     /locus_tag="ACHK07_002451"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO8102770.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDTLFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(29768..30883)
                     /gene="yliI"
                     /locus_tag="ACHK07_002452"
     CDS             complement(29768..30883)
                     /gene="yliI"
                     /locus_tag="ACHK07_002452"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO8102771.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(30994..31377)
                     /gene="bssR"
                     /locus_tag="ACHK07_002453"
     CDS             complement(30994..31377)
                     /gene="bssR"
                     /locus_tag="ACHK07_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO8102772.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            31590..32915
                     /gene="rimO"
                     /locus_tag="ACHK07_002454"
     CDS             31590..32915
                     /gene="rimO"
                     /locus_tag="ACHK07_002454"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO8102773.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(32962..34290)
                     /locus_tag="ACHK07_002455"
     CDS             complement(32962..34290)
                     /locus_tag="ACHK07_002455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8102774.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(34298..>36592)
                     /locus_tag="ACHK07_002456"
     CDS             complement(34298..>36592)
                     /locus_tag="ACHK07_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8102775.1"
                     /translation="AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIE
                     RSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVV
                     YHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFT
                     LRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKL
                     IIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFI
                     IIVLLVVYLLTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNS
                     IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAV
                     FGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDS
                     SFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEE
                     NIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIA
                     EDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIM
                     KENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEI
                     NPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQ
                     AQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         9504 a         9287 c         8559 g         9242 t
ORIGIN      
        1 aacatcacct aaactgccgt tttcaaaaca acattttttc aatatgcttt cattcacaac
       61 agtgtgtgat aaatagagat tacttatgtt ttgctttatt aatgccacgt cattaaaatg
      121 actatattta ataaagcaac catcattgag tagtgtttta agattgatta aattagttgc
      181 atcttcacgt accatcgtgg caacaggaac aggcaatgac atgaaaactt cttcattcac
      241 catcaagtga atcatgcatt gcgttccctt tgtcatatcc acacttacgc tacaaatatc
      301 gttgtaatgc gaagagtgca tttcaggaaa cagattaaac acggtatctt gaaaggcact
      361 aaatcgtgtt aataaatcct gataatgaac aggttttgtt tcaaaattat catcagcaaa
      421 tccaagcact attgcatgct ctacatctaa cgcagtttca aatgactctt cttctgtaaa
      481 atcattattt aaaacctcac ctgacgtatt atcactgtca taaaaagtgc tcatcaatgc
      541 gatattgttt gttacagcgt cttttgcaat attctcatta attgcatatc gcctgctata
      601 atcataaaaa tgaagaaacg cgtcctttat aaaggatata tattgttctt tgttaaagtc
      661 aatacgactg tataattttt caaaaatcac attcgcaatc ttactaccca tagtatgcct
      721 gtataataat gggtgcgatt caatcgtaaa atgcttgtct aattgatgtt gtaatttatt
      781 ctcatagtcc agtgggaaaa gaaacttttg tctttcaagt tttccattcg atatgttacg
      841 tattccaata gagattcttt cattacccgt ttttatttca acttcgttat cttctgattt
      901 acccagatat tttttaacat cagtctcgaa agcattacta aaaaatgtat taaattgata
      961 atccgctgtt ggaatatttt ccgcgagcaa cacatcattt tttatgagat cactgtcagc
     1021 atttattgct aatatcagat gcaagtagat ggtatcccat gtagacaact cacccttacg
     1081 actggaattt aatatcgcga aaaattccct cagtgaatcc aggaaattat cactttgatt
     1141 caattgtgtg catggaaatg atgacacttg atcagatata ttgtttatca tattaaaaat
     1201 cacccaagaa aaacgataat ccaccgaact cctagcgctt tattattgta aaagcacaac
     1261 accttttata ctcatattaa ttaacgaaac actgaaacaa cttgatttat tcttcgctaa
     1321 aattcacttg ttcaaaaata ttatatttta tacccacaac tgtagcataa ataatattat
     1381 caaaagtcaa agctgggact atcaaatttc gctcattttc aaaaatacaa cgtaattttg
     1441 gcaatcatct taatagcagg atctcttatc gcatgtgaaa aatttgtaat gattagacaa
     1501 taaccgcgtt catttatcac gcgttataaa tgtgaaactg gcttcttgca gatgctctta
     1561 acctctacag gaggatttat accaccattt cagaactatc atatttacct taaatgatca
     1621 tttcagccat ataacacgct caacctcaca aatggtttac actgtctatt gtgtgggtaa
     1681 tttaagtaag tcagctacga gcaaccttga gtgggtagcc agcaatggac tcaaacccaa
     1741 tcaggtgttc agattgtttc agaattgtat taaaaacaat gtattaaata atctgatagc
     1801 gtttggtcgt agtgtgacgc agacagcaag ctaataagga aatgattatg cgctaccgtt
     1861 cactatctct acttattcca tgtgcactgc tgctgagtgc ctgtaccacc gtcacgccag
     1921 cttataaaga taacggtccc cgcaccggtt cctgtgtcca gggggggcct gacagcgtgg
     1981 cccaacaatt ctatgactac cgcattcaac atcgcagcaa tgacattacc gccctgcgcc
     2041 cctatttgag tgacaaactg gcaaccctac ttagcgatgc cagccgcgat aatagtcatc
     2101 gtgaattgtt aagcagcgat ccgttctcca gtcgaaccac cttgccagac agcgcccacg
     2161 ttgccagtgc atcgactatc cctaatcgtg atgcccgtaa cattccattg cgcgtggatc
     2221 tcaagcaggg tgaccagggc tggcaggatg aagtgttaat gattcaggaa ggccagtgtt
     2281 gggtgatcga cgatgtgcgc tacttaggcg gtagcgttca tgccacggcg ggtacgctcc
     2341 gtcagtccat cgagaatcgc taacgccttg atcaaccaat aaaccccgta aattgtccgg
     2401 catttcgtga ggaatgccga catttatgct cgccgaccac cgcccccgtt attttgtgct
     2461 atgtttattg aataatgcgc tttgctttta acttttaaag cagaaatatt gcataattat
     2521 tctgtcaaag gtactatctt cgacctcaat agctatcaga ctgccagtat acgagtgtca
     2581 atgagtattc aattaaacgg cattaattgc ttctacggcg cgcatcaggc gctgttcgat
     2641 atcacgctgg attgcccaca gggcgaaacg ctggtgttac ttggccccag cggcgcgggt
     2701 aaaagctcgc tgctgcgtgt actcaatctg cttgagatgc cgcgctccgg tacgctcaac
     2761 attgcaggca accatttcga tttcaccaaa acaccctcag acaaagcgat tcgcgatttg
     2821 cgtcgtaacg ttggcatggt gtttcagcaa tacaacctgt ggccgcatct gaccgtgcag
     2881 caaaacctga ttgaagcgcc ctgccgtgta ctggggttga gtaaagatca ggcgctggcc
     2941 cgtgcagaaa aactgctgga acgtctgcgt ctcaaacctt atagcgatcg ttacccgctg
     3001 catctttctg gtggtcagca gcagcgtgtt gctattgccc gtgcgttaat gatggaaccg
     3061 caggtactgc tgtttgatga accgaccgcc gcactggacc cggaaattac cgcacaaatc
     3121 gtcagcatca ttcgtgagct ggcagaaacg aatattaccc aggtgatcgt cacccacgaa
     3181 gttgaagtgg cgcgtaaaac cgccagccga gtggtgtata tggaaaatgg tcatatcgta
     3241 gaacaaggcg acgcgagctg ctttaccgag ccgcaaaccg aagcatttaa aaactatctc
     3301 tctcactaag attcgggata acaacaatga aaaaagttct gattgccgcg ttaattgcag
     3361 gttttagtct ttccgccaca gctgccgaaa ccattcgttt tgctaccgaa gcctcctatc
     3421 ctccgtttga atcgattgat gcaaacaacc agatcgttgg ttttgacgtc gacctggcac
     3481 aggcactgtg taaagagatt gatgcaacct gtactttctc taaccaggcg tttgacagcc
     3541 tgatcccaag cctgaaattc cgtcgcgtag aagccgtgat ggcgggcatg gatatcactc
     3601 cggagcgtga aaagcaggtg ctgtttacca ccccgtacta tgacaactct gccctgtttg
     3661 tgggtcagca aggcaaatac accagtgttg atcagctgaa aggcaaaaaa gtcggcgttc
     3721 agaacgggac gacacaccag aagttcatta tggataagca cccggaaatc accaccgttc
     3781 cgtatgacag ctaccagaac gcaaaactgg atctgcaaaa tggtcgtatc gacagcgtat
     3841 ttggtgacac cgcagtggta accgaatggc tgaaagataa cccgaaactg gcggcagtgg
     3901 gcgataaagt gaccgataaa gattacttcg gtactggcct cggcatcgcg gtacgtcagg
     3961 gcaacactga actgcagcag aaactcaaca ctgcgctgga aaaagtgaag aaagatggca
     4021 cttacgaaac catctacaac aaatggttcc agaagtaatt cctgatgaat gaattttttc
     4081 ctttagcaag cgccgccggg atgaccgtcg gccttgccgt ttgtgcattg attgtcggac
     4141 tggcgctggc gatgttcttt gcggtgtggg agtcggcaaa atggcgtcct gtcgcgtggg
     4201 caggttcagc gctggtaacc attctacgtg gcctgccaga aattctggtg gtgctgttta
     4261 tctattttgg ctcctcacag ctgctgctga cgctttcgga tggcttcacc atcaatcttg
     4321 ggttcgtgca gatcccggtg cagatggaca ttgagaactt cgacgtgagt ccgttccttt
     4381 gtggtgtcat cgctctgtca ctgctgtatg ccgcctatgc ctcgcaaacg ctgcgcggcg
     4441 cgttgaaagc ggtaccggtg ggccagtggg aatctggtca ggcgctgggg ctgtcgaaat
     4501 cggctatctt tttccgtctg gtgatgccgc agatgtggcg tcatgcgctg cctggcctcg
     4561 gtaaccagtg gctggtgctg ctgaaagata ccgcgctggt cagtttgatt agtgtgaatg
     4621 atttaatgct acaaaccaaa agcatcgcta ctcgtaccca ggaaccattt acctggtaca
     4681 ttgtggcggc ggcgatttac ctggtgatca ccctgctcag tcagtacatt ctcaaacgca
     4741 ttgacctgcg cgcgacacgt tttgagcgga ggcccagcta atgtttgagt atttacccga
     4801 actgatgaaa gggctgcaca ccagcctgac gctaaccgtt gcctcgctga ttgtggcact
     4861 gattctggcg ttgattttta ccatcatcct gacgctgaaa acgccggtgc tggtgtggct
     4921 ggtgcggggt tatatcacgc tgtttaccgg tacaccgctg ctggtgcaga tcttcctgat
     4981 ttattacggg ccgggtcagt tcccgacgtt gcaggagtat ccggcactgt ggcatttgtt
     5041 gtcagaaccg tggttatgtg cgctgattgc gttgtcgctg aacagtgcgg cgtataccac
     5101 gcagctgttt tacggtgcaa ttcgtgcgat cccagaaggt cagtggcagt cctgtagcgc
     5161 cctgggaatg agcaaaaaag atacgctggc gatcctgctg ccgtatgcct ttaaacgctc
     5221 gctctcttct tattccaacg aagtggtgct ggtgttcaaa agtacctctc tggcttacac
     5281 cattacgctg atggaagtga tgggatacag ccagttgttg tacggacgca cctacgatgt
     5341 aatggtgttc ggtgcggcag ggattattta cctggtcgtt aacggcctgc tgacgctgat
     5401 gatgcgtctg atcgagcgca aagcactggc attcgaacgg cggaattaat gtagctgcgt
     5461 acattcgccg gggggtttgt aggtctgata agacgcgcca gcgtcgcatc agacatcagc
     5521 acggtgcctg tgccggatgc ggcgtggatg tcttatccgg cctacatagt gcataaattc
     5581 ttataataaa gacggacaac ttagtgagtt gtccgtcttt ttttatttca tgcaaattat
     5641 ttaatcatat ttattgcata taaattcact tgatggcatt gttatcccat gccgcagaca
     5701 cggccaaaaa tcataagatt gacagacggg agttccatca tgaaaaagtt agttcttgcc
     5761 gcattacttg cttcctttac tttcggtgct tctgccgcag agaaaatcaa ttttggcgtt
     5821 tcagccacct atccaccctt tgaatctata ggtgctaata atgagattgt cggctttgat
     5881 atcgacctgg caaaagcctt gtgcaaacaa atgcaggcag aatgtacttt tactaatcac
     5941 gctttcgaca gcctgatccc gtcactgaaa ttcaggaaat atgacgccgt aatctccggt
     6001 atggatatca caccagatcg tagcaagcag gtatcgttca ccacgcccta ttacgaaaac
     6061 tcagccgtcg tgattgccaa aaaagatacc tacaaaacgt ttgccgacct gaaaggcaaa
     6121 cgcattggga tggaaaacgg tactacgcac cagaaatata ttcaggatca gcacccggaa
     6181 gtgaaaacgg tctcctatga cagttatcaa aatgccttta tcgatctgaa aaatggccgt
     6241 attgatggcg tatttggtga cactgcggtg gttaatgaat ggctgaaaac caatccgcaa
     6301 ctgggcgttg ctactgaaaa agtgaccgat ccgcaatact ttggcaccgg tctgggtatc
     6361 gctgtacgtc cggataacaa agctctgctg gaaaaactga ataacgcgct ggcagcaatt
     6421 aaagctgacg gtacatatca gaaaatcagc gatcagtggt tcccgcaata attattcatt
     6481 tgccggagtg ggcgaacacc cactccggca aaatcacttc tctacgctaa tcccaggcgc
     6541 tgaaactgct ctggcggcgc atcattatct ttcatcaact gaatcagcaa cgcgatcatc
     6601 cgggactcga tcacatcatc atgaatcgga tgtagctgct gcggatcgtt ttgcagatca
     6661 aacagcaaat gaccaaactg attcaacccc ttatattttt cctgtgccgg gatgcgtaat
     6721 acctgtaacc ctttggtaaa acgaaacggt ggggccagcg ataaagcccg catctcatcc
     6781 accggatagc ggctgttcat tttgcaggac atcaacgtgt agttggcaat atcatcctca
     6841 cgccccggca gcgctgcacg catatacaca taacgcccat cagttacgtt agcatgacca
     6901 ctgaaaacac caaacatcgc cgagctgcgt actggttcat cgtgactgac cgttttcgcc
     6961 agcggttgcc cctgcacatc gggcggcact ggctgttgaa aaaagtcata caacgttggc
     7021 gcccagtcga tcatttgtac cagtgcctgt cgccgctctc cttttacacc actgcgcggg
     7081 tcccagataa acaacggatt attggcaact tcgttgtaat aaggcatctg gtttttcgcc
     7141 caccagccat gctcaccgag taaaaagccg tggtcagtgc cgacaatcag catggtatct
     7201 cgccacagat cgtgctcatc catcaggtcg agaatgcgcc ccaggttgcg atcgcacatc
     7261 gacaccagcg aacgataccg gcaacggatg tgctccacgg cctcgtcgct ttcctgcacc
     7321 cggccacgcg gccagtcata atgcggtcca tcccagttat cttcatacaa ggaaagatac
     7381 tcttccgttg tgtgaaacgg ctcgtgcgga tcaaacgttt cgatctgcaa taaccagtta
     7441 tcttccgcgt gatttttgtg gataaaggca cagcctgcat caaacacttt agtttgtgga
     7501 aaatccgctt cctgctgaat atattcccgg tttgcccagt catggcgcca cagaccagaa
     7561 acaccgccac cggtctgctt ttgcggcacc cgtagcacct cgggaatagg cggttgcgca
     7621 acgcttgcat gccagtgatc gccttcctgt ccgcgcacaa tctcccacga actgtagcga
     7681 ttatgataat tcccgccgcc atcttcccag taatgcaaat gatcgcttat caggtgggta
     7741 tagatccccg cctttttaag cagctccggc atggaatcgt caaacggttc taacggcccc
     7801 cactcccgat gcaggaagtt acatcgcccg gtatgcaact cccgccgggc aggcatacac
     7861 ggcatactgc cgacgtaact gttttcaaag gtggcggcat gagccgccag acgttggaaa
     7921 ttaggcgctt tggttaacgc atcgccataa ggtggcagat aacgtttatt cagactatcg
     7981 aacagcagaa taatcgctct cattacgccc taccttattg attaattaaa tgcggaaaat
     8041 gttgctgaac cacttcataa atttgctgac caatagtttc accgtccata atattctcta
     8101 tcggaatcaa atattgccct tgtttcgggt caagactaac cacatcgagt aattttttgg
     8161 aaatagcaaa aatatccgcc cagtcggtaa atccgccgac ttcacctgcg gcggtatgtt
     8221 ccatttcaac tttgaaatca tatttggtca gcgctttttt aacgaccgtt tgtaacataa
     8281 cgctactgcc tacaccgtaa ccacagacaa acagaatatt aattgtatct ttcattttta
     8341 aacccttaat atttaattaa cccgcgtaag gaccaagatg gaatgttgca gcgataaatt
     8401 tcagaacttc acaaatagca ggccatacgg ttgcccagtc aaatattcct gtccagccga
     8461 cgccattttc agcaagcccg gttaacggaa tagcccacac cgtaccgaag gtctgaataa
     8521 tacccagcaa gaacgtacag ataattacgg aacggtatcc gcccatgcgg tttgctaata
     8581 cgccaattgg accgccagag aaaaacagag gtacaaagcc aggcaatacc ataattgggc
     8641 tgtgaaccag taataagatc cccatcgaaa aaatggtgcc aaaagtacag aagacgaagc
     8701 ctaacgttgc ggcattgggt gaaaaaggta acagcgctgc aacatcgaca gcaggaatag
     8761 cgttaggaat aaagcgatct tgccagcctt taaacgaacc gttaatttcg cccaccatca
     8821 tgcgcacacc ctgcagcaga atgaccatat acatggagaa ctgtaagcca aggttgatga
     8881 tatagatgta ccagggcttg cctttcgcca ttaactggac attatcgata cccgttgata
     8941 gcaagaaacc accgacaaac agcgtcatca caatggcgat agccgttacg ttatggttaa
     9001 aaatcgccag ccacccggga agtttcaggt tttcagcatc gtgcttttca gggtcaccaa
     9061 atttatgggc gaatttggag aaaaaccaca atcccaacat ctggttatga gcaatggtaa
     9121 aaccggcatc tttggtaact ttggcaatgg gcttaacaat gagcgtggtg gagaacgccc
     9181 agaatacacc cgttaatatt ccggcaataa taatggatgt agtccagcta aaacccagcc
     9241 aaaagacaat caaccataat accgcctgag aatgtgaaac accggtattc cctgtcaaaa
     9301 atatcccttt ggcaccagta taccgaccaa ataataccag cagcaggttg gtaaaaaacg
     9361 ccagtacaaa tacataaccg acataaccaa aattatcccc caacgcaatt tgcgttgccg
     9421 ctttcattgc gtaggaatcc attaaatatc cgtgaacacc ataggcttta cttacggctt
     9481 ctgctaccgg tttaaatgtt gcggaaaact ggaaaccacc aaataacacc atcattaatc
     9541 ccaccatggc gctgaccatg ccggtaataa tttttattgg cgatttctta tcaagggcat
     9601 aaccaatacc caccagcaaa ccaattaaaa ttgccggttc agttaaaaac gtttgaataa
     9661 catctatgaa tttggcaaac atcgtcggaa cgccctccat aagcgtacat ttccgttgtc
     9721 aatgcatgaa ccttctgctg acgttaataa tgtctcagga aattttgtgt ttcgctaagc
     9781 aatgcctctg tatgtattcg gtaagtgcac tgtagtaatc aaaacagttc aattaaagat
     9841 cttatttgat tatttgtgat ttgatttgca aaaatagagc aatatataac cgtgatcgat
     9901 cggttattat taatcgatcg atcacgatta ttattttgga tggatgtatg ccgaggtgat
     9961 attgatttga atgatgtatt tacattttta ccagcagcgt cagcacttca tagtgcgcgg
    10021 tgtgcgggaa catatcgaaa agctgcaccc gttcaatgcg ataaccaggc agttcgcgga
    10081 tatctttcgc catggtttgg gcgttacagc tggagtagat gataaaacgc ggtgccatcg
    10141 ttgagagata atcacacagc ggtttaccaa tgccgcggcg cggcgggtta accagcacca
    10201 gctccggcac ctccccctga gcggtggcaa actgagtgga gtccagcgcc tgaaattgca
    10261 aacgcgttaa gcccagttca gcagctgact gctttgcaca ggcaatggcc tctggcgcaa
    10321 tttcgatccc ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag ccccccacgc
    10381 cgcagaacag atcccacata tgtttaaccg gcagctgtcg tacccagtcg cgcgcggtag
    10441 cgtacagctg gctggcgacc gccggattag tctggaagaa actttgcgga cggatccaca
    10501 gcggcacatc gttaaaacgc tctgccagcg cctgttgttc ggtcaggtag atctccgtct
    10561 ccccttccat aatcgccata tgtaccggct gaatattgac ggtgataact tttagctgcg
    10621 gcaattgtgc ctgtaaccac ggcaatgcct tacgcagttg tgccagtttg gtttcagaac
    10681 gcagtacaaa gcgcagcatc atgccgccat cgctctggct ttcagtcagc agaatgtatt
    10741 tcagttcgcc acgcttacgc gccacgttgt agggcgttaa ccccgctcgg gcgataaacg
    10801 gttttagcgc cgcaaaaacg ggcgcaaatg aggcaggata aagcgggcag tcacaaaggt
    10861 cttccggtgt gccgtcgcga tgcagcatac cgagcagtgg tttttcaacg ctaccactca
    10921 ccaccatttt ggctttatta cgaaaccctt gttccgggcc tgacaccggc gcgcaccatt
    10981 cctcaaccgg aaagtcggcg agcagatttt taagatcggc ggttttagcg gagagttgct
    11041 ctggaatcgg ctgcgttatc cactgacagg aacgacagcg acccgcgtcg taaagtgcgc
    11101 actgcataca ttgaccttca catcatcagg ggcgacgatt atacacatta ttgcaactgg
    11161 aagaaccgcc gactggtcga gggaacgaac agcagcatca ggatgagcat atccggcagc
    11221 ttctgcagca tcaggctatg gaagatttca cgttttgatt caccgggaat gctgaacagc
    11281 tccggataac cgtaccccag cgaagccgcc cacaagtaac ttgcggccgt gatttgtgtc
    11341 agcagatata gccatcgcgc ccagcgacgc cctttcacca gtgaaaacgc acaccagatc
    11401 tcaatgaaaa ccagcaccag actgcttaaa aagaccagcg ttaaactcca ggtttgtacg
    11461 ctgcgatgaa tgaactcgcc aataccgcgc acgcccagcg tattgaaaat catcagcacg
    11521 tcgaggccac ggatcataat aatggcgagc gccgccacct gcaccagcgc aggcacattc
    11581 aggcgagcat gagatgaaga tgttttctta aaaaatccca acgtttcgtc ttccatgaaa
    11641 acaatgccgc gacatacgcg gcattatgta gccaggttgg caaattttag tgtcttcagc
    11701 cacgtcttgc acgctggatg tcgcgtatcc gctgtttttc tgcgcgagcc atcagatacc
    11761 aggcgataaa tccgacaatt ccgaccgcgc cgaggatcaa tgttgccagg gcgttgattt
    11821 ccggattcac ccccatccgc acgctggaaa agaccagcat cggtaacgtg gtggctcccg
    11881 gcccggaaac aaagctggcg atcaccagat catcaagcga caaagtaaaa gccagtaacc
    11941 agccagaaat gatcgcgggc atgatcatcg gtagcgtaat gacgaaaaac actttcagcg
    12001 gcgtcgcacc gagatccatc gctgcttctt ctatcgagcg atccagttcc cgcagacgcg
    12061 acgaaataac gactgccaca taagccgtac agaaagtgac atgcgccagc cagatggtaa
    12121 gcataccgcg ttccgcaggc cagccaatgg catgagcaag cgcgacgaat aataacaaca
    12181 gcgacaagcc cgtgatgaca tctggcatta ccagcggcgc ggtgatcata aaggcaaagc
    12241 catttgatcc gcgaaacctg ccaaagcgca ccagcaccac cgccgcaata gttccgagga
    12301 tcgccgccgc cgtcgccgca caggccgcaa ttgtcagact taaaccaacc gcactcatca
    12361 tcgcgtcatc gcgcaataat tcgccatacc agcgcgttga ccagccggcc cacaccgtca
    12421 ccagcttcga gctgttaaac gaatagataa ccagcatcag cattggcgcg tagagaaagg
    12481 tgaagcccag cagcaaaatc acaatccgcc agggcgaacg aactaccggt aaattattca
    12541 tccgtgttct cccacgcttt tttgctggtg tttgtggaac cacattatcg gcacaattag
    12601 cagcagcaac atgatgatcg ctaccgccga ggccaccggc cagtcgcggt tattgaaaaa
    12661 ctcttgccat agcacgcgcc cgatcatgat gctgtccggg ccaccgagca gttccgggat
    12721 cacaaactcg cccacagccg ggataaacac cagcatcgat ccggcaataa tcccaccttt
    12781 agtcagcggc acgatcacag taaagaacgt tttcagcggt cgtgcaccga gatccagcgc
    12841 tgcttccacc agcgaataat caatacgaat caacgcggta taaatcggca gcaccataaa
    12901 cggcacgtag gcgtaaacaa tgccaatata aactgccaga ttggtatgaa gaatggtcag
    12961 cggttgatcg ataaccccca gccacagcag aaaattattc agcacaccgt tgttttttaa
    13021 tattcccatc caggcgtaga cacgaatcag aaacgaggtc cacgacggca ggatcaccag
    13081 taataataag atattacggg tcgaaggctt actgtgcgcc accgcccacg ccagcggata
    13141 gccgatcagt aaacagcaaa tagtcgaaat cgccgccacc tgtaacgact ggagataagc
    13201 atcgaaatag agcggatcgt cggtcagttg cagaaaatta ccgagattaa gagtgatgga
    13261 aaattgcccg tcagcccact ccatcagttc ggtataaggt ggaatagcgc gcgccatctc
    13321 tgccaggctt attttaaaga caatcagaaa tggcagcaga aacagcaaga tcaaccagat
    13381 atacggcaac gcaatgacca gtttgcgccc atggttcatt tgcaactgcg acaaccacag
    13441 cttaaatccg cccggcggtt tcgactgggc agcaggttca agtgtactca tctttgctcc
    13501 ttaaaccgta agcaccacac agctgtccac ttcccagcac aaacgcactt cgtcgcccca
    13561 ggtcggtaac cctttacgat gacgatgggc gttttgtagc tgggcgctaa tcatctgccc
    13621 acttttcaga cgaacgtgat acaccgaaag atcgccgaga taggcaatgt gtatcacctc
    13681 ccccaccgcg aagttacaac cattggcggg cggctcttcg caaagcatga ttttttccgg
    13741 acgcagcgct acgtgtaccg gcacgttatc gaccactgaa gcatccgcat cgactttcag
    13801 tggatgcacc agccccggcg aataaagcac cagaccatct tcctgacgct ctttgagtac
    13861 accttcaaag acattaaccg agccaataaa ttcggcgcta tagcgggtag tcggatgctc
    13921 gtagatctct tccggttcgc caatctggac aaatttccca cgattcataa tggcgatacg
    13981 ccccgccatg gtcatcgcct cttcctgatc gtgagtaacc atcacgcagg tcacaccgac
    14041 gcgctccaga atatccacca cttcaagctg catccggtca cgcagctttt tatccagcgc
    14101 gcccatcggc tcatcgagca gtaatagttt cgggcgtttg gcaaggcttc gggccagggc
    14161 cacacgctgt cgctgaccac cggaaagctg atgcggtttg cgtttggcga actcctgcat
    14221 atgcacaagg ccgagcatct cattgacccg gctggcaatt tccgctttcg gtagtttgtc
    14281 ctgtttcagg ccaaaagcga tgttctgttc cacggtcata tgcggaaaca gcgcgtaaga
    14341 ctgaaacatc atattgatgg ggcgcaggta aggcggcacc tgtgacaaat cgacgccatc
    14401 gagcataatc tgcccggctg aaggttgttc gaaacctgcc agcatacgca gcagcgtgga
    14461 cttgccacag ccggatgcgc ccagcagcgc gaagatttca cctttgtaga tggtcaggct
    14521 gacatcatcc accgcatgtt gaccatcgta ggatttggtc aggttgcgga tttctaatag
    14581 cggcgtcagc gccttacggg ttttcgcctg cgggcgaggg atagcgtcat tcacggctgc
    14641 tctccggcat tgaatgtgtg caagcgcacc ataatggtgc gaattgccgg cgggtgtggt
    14701 gcgcccgtcc ggcatctaag gctgcggatt attttccgct cttcactttg gtccacgcgc
    14761 gggtgcgcac acggtcgatt ttcggatcct gtactttcag agtgaacaac ttcgcgcgga
    14821 catccgcagg cggataaata cccgggttat cacggacttc cgcactcacc agcggcgtgg
    14881 ctgctttatt ggcgttagca tagaacacat ggtcagagat atgtgccacc acatcaggac
    14941 gcagcaggta attcaggaac tgataggctt cgtctttatt tttggcatcc gcaggcatgg
    15001 cgaatacatc aaagaacgcc atcgcccctt cttttggaat cgagaacgag acattcacgc
    15061 cattcttcgc ttccttcgcg cggtttgacg cctgccagac atcacctgcc cagccgatag
    15121 cgacgcaaat atcgccgttt gccaggtcgt taatgtattg agatgaatgg aaataacgaa
    15181 tgttcgggcg cagctttaac agcagatcgg tagccggtcc ggtgtaatca tccgctttag
    15241 tgctgttggg atctttgccg agataattca gcacggtagc aaaaacttct tctggcgcat
    15301 cgaggaaaga tacaccgcag cttttcagtt tttccagatt ttcaggcttg aggatcaagt
    15361 cccagctatc gacgggcgcg ttttcgccca gcaccgcttt gactttatcg acgttatagc
    15421 caatcccggt cgtcgcccac atatagggca tagcaaattt attgtcggga tcgtgtttgg
    15481 cgaccaactt cagcagttcc gggtcgagat tcttccactc cggcaatttg cttttatcca
    15541 gcggctggaa aactcccgct gtcagctggc gttcaagaaa gcttgctgac ggcaccacca
    15601 ggtcaaagcc ggtgctcccg gccattaatt tgccttccag tacttcgtta gagtcgaaaa
    15661 catcgtagac gactttaata ccggtttctt tttcaaagtt agccaccgtg tcgggggcga
    15721 tataatcaga ccagttataa atgtggagtg ttttttgttc agccgcgagc gtgccgacag
    15781 agacggccat cagagctccc gcaaccagac ccgaaagcca ttttttattt aaggcggtca
    15841 tatctctttc cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca ctgaaagata
    15901 tgcaataaac gtgcatattt tttatcggcg cggtgtaaga aaagagaact ctctcccggc
    15961 cagcactgct cgctatataa aagcatcgca agaataactt tagccagggt ttgagactat
    16021 agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct atgtgataaa
    16081 gccaaatcga cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa cagcagataa
    16141 ttttgcagcg cgcagccact gcaccagcga ctgcgtgctc gaaaggagtt atcagtggag
    16201 gaaatagttt tcggtaacgt tgttttgccc ttcatcgtca gtcggtaaca gcagttgatg
    16261 ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc
    16321 aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca ccagctttgg
    16381 cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg cattgatggc
    16441 agataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa
    16501 caggatcgtg ttatcgatca gatcgatttt ggcatcaaag acaccgtcga aattctgcat
    16561 atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc ccaggtcatc
    16621 gagccattga cgcagcgtat ccagaccagg aacgaccagc gatgtcataa ttttgtacaa
    16681 cctctttcga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa accatgatca
    16741 gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag cgtggcgttc
    16801 gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta ttccttccag
    16861 ccccggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca gaatatccac
    16921 accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg
    16981 tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc
    17041 tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca acaaccagac agcggatatc
    17101 gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca gaccgcggaa
    17161 agcgtcaatc acgctttccg ccgcctgacg tgtctccgcc agcacgacgc caattccctg
    17221 cgtgccttca accaacttga ccaccagagg cgcaccaccg accatgtcga ttaaatcgct
    17281 ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct gacgcgccag
    17341 cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg
    17401 atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg taatggcggt
    17461 gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt agtgaataga
    17521 agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt caaccaggtg
    17581 accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc catcccggga
    17641 caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca
    17701 tacatcgacg tggcggtttt agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat
    17761 aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg tatcccggcg
    17821 attattgctg ccacgggtga ggtaatattc cgccagttgc tcgtcatact gcgccagtgc
    17881 gtctttatcc agcggttgat agctgttttc atgcaccaaa atggaggccg gtaaacgcgg
    17941 cttgagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg gcagaacatg
    18001 ctgcggtaat ttcagcagtt tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata
    18061 tacgccgccc aatcccagcg attccgctgc ggttaatgca ttctgcgcca tcattgccgt
    18121 atcaacgaca ccgagcaaca gctgttccgc caggccgagc tgagcatccg gacagatctg
    18181 taaatggcgg ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg ctacgtgttt
    18241 ttgcccgccg gtcagcgtca ccagctcttc acgtaatgct ttgtcggtaa tgcgaataat
    18301 gctactacac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc
    18361 acgctgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat ggccacaaat
    18421 aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat tgcggtggac
    18481 ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc accgcagcgg
    18541 gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc cgagaaaatg
    18601 gctcaggcag cgacagggag tcgcttaccg acagcagcgc caccgccagc aacatcattc
    18661 cgataaattc cagtatcaac acgcttttag gcaatttacc gatcgcgcgc atacgcttcc
    18721 ctctgcaaag tgagccttca gtctaaaact tttcactgta ttgtgtttaa cagttatagc
    18781 ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt agcgtgatca
    18841 ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg gtcgcccggg
    18901 ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg aacgcgatga
    18961 ttttcagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag atctacaaca
    19021 aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg
    19081 cggctatacc gattttgctg catgggtgaa agagaatctg gacgcctgat cgtctgacaa
    19141 gccctcgcgt tgagggcttt actgattttt tctgtgctgt ggtttaaaca agctactgat
    19201 aaataagaaa cacagtgccc ccagcgcaca ccagaacacc gcgcttagta accatgccag
    19261 ctcttgccag aatgagcggg tcggtgaaaa aaacagccgc ataataagca ttgaacaggg
    19321 tgccgccagc attgcgccaa atagaggttt caggacttct ctacgctgtg aaaagaaact
    19381 ggcaactgct ccaggaagaa tgaaaaacag caagccgatt tcaggatgcc cggcagcccg
    19441 aaaagcgcct ttcatgtgcg tcgccagaaa aaggcacacc acaatgaaga ggacaaaaca
    19501 gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac actgcgtcta
    19561 tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg cattccatgc
    19621 taatataggc caacgcaatt catatagccg ttgataccta atgtgattac actagtaaaa
    19681 tatattgtta ctttactatc gtttaggtgc gctgaatgaa tctgcgccct gaattctggt
    19741 aaaaaacatt atcgtaaatt accatttctt tcaacagctt actagtaaac aagaagttag
    19801 cctccgtgaa tataaacgtc gccgaattgt taaatgggaa ttacattctg ttattatttg
    19861 tggtcctcgc gcttgggcta tgtctcggaa agttacgact tggttcgatc caactgggta
    19921 attccattgg cgttttagtc gtatcgctgt tattaggcca acaacatttc agcattaaca
    19981 ccgatgcgct taatcttggc tttatgctgt ttattttctg cgtcggggtc gaagccggac
    20041 cgaacttttt ttccattttt tttcgcgatg ggaaaaatta cctaatgtta gcactggtga
    20101 tggttggcag tgcgctggtg atcgccttag ggttaggtaa gctgtttggc tgggatattg
    20161 gcctgacggc cggtatgtta gcaggctcta tgacgtcgac accggttctg gtcggtgctg
    20221 gcgatacact gcgtcattcc ggcatggaaa gcaggcagct ctcactggca ctggataatc
    20281 tgagcctcgg gtatgcctta acctatttaa tcggtctggt gagtttgatt gttggtgcgc
    20341 gttacttgcc gaaattgcag catcaggact tacagaccag cgcccagcaa atcgcccgcg
    20401 aacgtggcct ggacactgat gccaaccgta aggtttattt accggtgatc cgcgcctatc
    20461 gcgtcggccc ggagctggtg gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt
    20521 atcgacaaac cggctgctac attgaacgta ttcgacgtaa cgggattctg gcaaatccag
    20581 acggtgatgc cgtgctacaa atgggcgatg aaatagcgtt ggtaggctat cccgacgccc
    20641 atgcccgact cgatcccagc ttccgtaacg gcaaagaagt tttcgatcgt gaccttctcg
    20701 acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca taacgccgtt ggcaaacgtc
    20761 tcgcacaact gaagttgacc gatcacggtt gcttccttaa ccgcgttatt cgtagccaga
    20821 ttgagatgcc gatagatgac aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg
    20881 gcgatgcccg ccgcgtaaaa accatcgccg atcgcatcgg ctttatctcg attcacagcc
    20941 aggtcactga cttgctggca ttctgcgcct tctttgttat tgggctgatg atcgggatga
    21001 tcacattcca gttcagcaca ttcagtttcg gcatggggaa cgctgccggg ttgttattcg
    21061 ccggaattat gctgggcttt atgcgtgcta accacccgac cttcggttac attccgcagg
    21121 gtgcattaag catggtgaaa gagttcggct taatggtgtt tatggcaggc gttggtctga
    21181 gcgccggtag cggtattaat aacggtctgg gcgcgattgg cggtcagatg ttgattgccg
    21241 gattgattgt cagtcttgtg cccgtggtta tctgtttctt gttcggtgct tatgtattgc
    21301 gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa
    21361 tggagatcat cagtgataca gctcgcagta acatcccggc gctgggctat gcgggcacct
    21421 acgcaatcgc caacgtcctg ctgacgctgg cagggacaat catcgtcatg gtatggccag
    21481 gattaggata aaactgaagt tgccctgaaa atgaaatttt tttgcacaac cgcagaactt
    21541 ttccgcaggg catcagtctt aattagtgcc actgcttttc tttgatgtcc ccattttgtg
    21601 gagcccatca accccgccat ttcggttcaa ggttggtggg ttttttgttg cctaaaattt
    21661 atctactacc cagcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta
    21721 cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt
    21781 ccgggttcaa accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc
    21841 gtttttaata gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatgtta
    21901 tccggcgttg ccacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga
    21961 gcatcaataa catcgctaaa aaatgcctga tattgccggg acatgatctc agtaaaactg
    22021 ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac
    22081 ggtaccccgg caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc
    22141 accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc
    22201 atgtcatttc ctctatctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt
    22261 ccctcttctt attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg
    22321 ccgaacctgg gcgctgttta tgttcttctt tttgccgggc ctgttaatgg cgtcctgggc
    22381 aacccgtacg cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt
    22441 tctctttggt ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa
    22501 acgctttggg acacgtaatg tcatcctggt caccatgtcc tgcgcattgg tcgggatgat
    22561 gatattaagt ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt
    22621 ctttggggca agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga
    22681 gcgagaaatg aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct
    22741 ggcaggcgct ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat
    22801 tttattggcg gcgctggtag gcatcgcgcc tatatatatc gccattcagg caatccctga
    22861 cggtacgggc aaaaatgctg ccgatggcac ccagcatggc gaaaaaggcg taccttttta
    22921 tcgcgatatc cagttgctgc tgattggtgt tgtggtgctg gcgatggcct ttgccgaagg
    22981 ttctgccaac gactggttac ccttattaat ggttgacggt cacggtttta gccccacttc
    23041 cggctcgctg atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg
    23101 ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcgc taatgggggc
    23161 gttgggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt
    23221 actctgggga ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac
    23281 cggccccgat gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct
    23341 cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct
    23401 ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga
    23461 taccaaaacg cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac
    23521 atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag
    23581 tatcagcaaa tgaaagcaca agacattcgc tttgtggtcg ccagcgggaa tcaatattat
    23641 cagttgatct ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc
    23701 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
    23761 actactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgtggaaaa
    23821 aatagcgcct atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
    23881 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatctt ctttaagttt
    23941 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
    24001 ggcgatatta tggtgccggt ccacaccggc aacggcagca tcgatctgat tatccccggc
    24061 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
    24121 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
    24181 tttgcaatgg aaaatgccgg caacgcggtc gtcgcagcgg caaaataccg ggcaggctcc
    24241 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
    24301 gaccaataaa aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc
    24361 gcggaaatta acccgctgca ttatttcagg cgaaatgttt atgaaaaatt gtctactgtt
    24421 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
    24481 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
    24541 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
    24601 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
    24661 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
    24721 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
    24781 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
    24841 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
    24901 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
    24961 ttcgccagac caataccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
    25021 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
    25081 cccataataa tcagcgaacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta
    25141 cccgcaatta acgccccgaa aataggcact tgcagcaaac catattcata gctgctcaac
    25201 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgcgagcaa cggcaggcta
    25261 acgaatccca gcgccagcgc ccccgccaca aagcgaccgt tcttcagcac cagcttatag
    25321 tcacgaccga gttctttcag cgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
    25381 gctcgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
    25441 tcccagggca gcacatggat ccacgccgcg cccaccagtg gaccaagtag cggagcaatc
    25501 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
    25561 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
    25621 aacagggtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gacaataaac
    25681 cacaccacac ccgccagcat caccggacgg cgaccaatac gatccgacaa cggccccagg
    25741 agccattgta aaaacatccc gcccgccaga tatgcggtca tcgaagtagg aacccaatca
    25801 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
    25861 atataggttg aaaattcgta cagcaccaga cagagaggga aaagtaacgc ctgacgtcca
    25921 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
    25981 aaagtttaat aaaagcgcag ctaacgagaa agcgaatttt gtagctgaaa ccgcggttaa
    26041 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
    26101 cccgttacaa tcacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
    26161 cccagtttaa cctgcacttt attctcaacg agttgcctgt attggctccc ttttaatcac
    26221 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
    26281 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
    26341 taccgtggtg ccgctgctgg ccgcggtact ttggttgtgg gggcttacag cacaacggca
    26401 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
    26461 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
    26521 tgcggcggat gactcattcc cgagcgatca cggtacggtg attttcacct ttgcactggc
    26581 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
    26641 tgcctggtcg cgcgtttatc ttggcgtcca ctggccgctg gatatgctcg gtggattgct
    26701 ggcaggtatg attggctgcc ttagtgccca gattatctgg caagcgacag ggcataaact
    26761 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
    26821 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
    26881 cagagggatt atggaaacac gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa
    26941 acgcagcgat aagttacatc ttaaagacgc cgccgcgctg cttggggttt cggagatgac
    27001 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
    27061 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
    27121 agaaaaacgc cgggcggcaa aactggctgc gacgctggta gaacccgatc agaccctctt
    27181 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaataccttt
    27241 caccgccgtt tgttattcgc taaatacctt tctggcgctg aaagaaaaac cccattgccg
    27301 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
    27361 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
    27421 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
    27481 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
    27541 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gtccggaaga
    27601 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
    27661 cggatgacgt ttcgcaccat ccggttatca gaagattaag agaaccagct gccgaaccac
    27721 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
    27781 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
    27841 gtgccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
    27901 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
    27961 ccgagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
    28021 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
    28081 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
    28141 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
    28201 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
    28261 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
    28321 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
    28381 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
    28441 ccaataaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
    28501 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
    28561 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
    28621 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
    28681 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcatt gccttctgcc
    28741 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
    28801 tcaacggttt gttcggccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
    28861 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
    28921 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
    28981 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
    29041 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
    29101 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
    29161 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
    29221 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
    29281 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
    29341 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgcctgtgga
    29401 tcgactcacc ggcacgtcgt gcggaagcag aaaaatggat ggactgggca aaccagacac
    29461 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
    29521 atcaggccgc cattgatgcc agttgcaaag agtgcgacac cctgtttgcc ctgctcgatg
    29581 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
    29641 tcgcaccgtt catctacaat ttgtttaatg ttggcctgac ctggacaccg cgtccaaatc
    29701 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
    29761 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
    29821 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
    29881 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
    29941 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
    30001 aggccatgcc gctcacggcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
    30061 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
    30121 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
    30181 ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt ccttgcggat
    30241 tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata aaaggattat
    30301 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
    30361 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
    30421 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcgaaaga
    30481 cggtgcgaaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
    30541 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
    30601 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
    30661 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
    30721 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
    30781 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
    30841 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
    30901 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
    30961 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
    31021 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
    31081 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
    31141 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
    31201 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
    31261 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cggcgaggtc
    31321 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
    31381 tcgatcctcc tctttccagc cttgttacaa ttaaatagta tggctatttt ttgagcaaaa
    31441 tgctgtccag gatcaattct tcactccgaa ttaacaaaat ttacatttcc ttcgcctccg
    31501 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
    31561 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
    31621 gctttgtttc ccttggctgt ccgaaaaacc ttgttgattc agagcgtatt ctcaccgaac
    31681 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
    31741 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
    31801 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
    31861 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
    31921 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
    31981 aacaaggtgt caaactgacg ccgcgtcatt atgcctatct gaaaatttct gaaggctgta
    32041 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
    32101 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
    32161 tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact ggcttccaca
    32221 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
    32281 tctggacacg tctgcactac gtttatcctt atccgcatgt ggacgacgtc atcccactga
    32341 tggcggaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
    32401 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
    32461 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
    32521 agacggaaga agatttccag atgctactcg acttcctgaa agaagcacgt ctggaccgcg
    32581 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
    32641 ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
    32701 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
    32761 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
    32821 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
    32881 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
    32941 tgtccggcct gcgaataatg attactgcca gctaatctgt gccctgccgt ttttcttact
    33001 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtagtac attcatttga
    33061 atgcgctaca ccgatactgg tgcttaaatt aatgcttccc acctggaaat gaacgatttc
    33121 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
    33181 accgccgaag cggtagactt tacctttatc taccgggatc tgtgatttaa ggattttcac
    33241 cactttcatc aacacttcat cacccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
    33301 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
    33361 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
    33421 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
    33481 aaaatggtta ttaataatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
    33541 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    33601 tgtacaaacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    33661 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cattatattc
    33721 atcaccggtc accggtttac cgttcttaaa cagagagaac atcaggttct cgctatccat
    33781 cgccagagat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    33841 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    33901 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    33961 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    34021 ataagagggt gaaaccgagt cggcgtaagc tgtgcgtaag ccaaccaaca gctgttcctc
    34081 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    34141 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    34201 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    34261 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    34321 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    34381 atcctaattt gattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    34441 ggatccggtt atagcgtgcc aggtcgaaaa taatgcgcac aatttttccc gcagtaccga
    34501 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    34561 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    34621 gcaattgttc aatattgaga taaaaatgtt cattaattcg ttcgattacg gtttcagtga
    34681 tctcaacaca gaggctgttc gccttcaggc cattttcttt cataatggtg gtaaaacgct
    34741 catgaaagtg tggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    34801 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    34861 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    34921 aaatgatccc ccgctccgca gacacccaac ggcacaacgc ctccgcgccc agaatggcgc
    34981 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    35041 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    35101 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    35161 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    35221 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    35281 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    35341 cgcagcattg tgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    35401 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    35461 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aacccacttt
    35521 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    35581 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    35641 gagcctcgat ctgatccgtt gagtcaggca aaaacgccag cgtattaacc agctctgtca
    35701 tttcagaaaa ttgccgacgg aacacacgag tggtcagtaa ataaaccacc agcaatacga
    35761 taataatgaa cgcaaacggc agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    35821 ccggataata aaaatagatt tcgaataatg cgttatttgg caccgctgat gttttttcga
    35881 tatattcagt atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca
    35941 gtttttcacg agcgagttga ttcttggcaa tgatctcatg gccttgtttc aaaaatagaa
    36001 cttcatcatt agcgctgatt tgatcaagat actcgttcag atatcgccag tttaaatcga
    36061 catgaatttt atccaccgct aacaaatcgt gatctttgcc gttgatgcgt agcgtaaaag
    36121 agatacattt tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag
    36181 agtcatctga agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc
    36241 gccctgactc gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat
    36301 cgttcgccct ccgcgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt
    36361 ctaaaaatgg catcatttcc attttcagga tcacagcgcg ttgaatctcc tctgtatttt
    36421 ttaataaata cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat
    36481 agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttccg
    36541 ataccagttg atagttaatg atgaaaaaaa ggccaataaa tgacagtgct gc
//
LOCUS       DAZGCQ010000046        36206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000046 DAZGCQ010000000
VERSION     DAZGCQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.046"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..2646
                     /locus_tag="ACHK07_002457"
                     /pseudo
     CDS             1..2646
                     /locus_tag="ACHK07_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
     gene            5539..6399
                     /gene="pdxI"
                     /locus_tag="ACHK07_002458"
     CDS             5539..6399
                     /gene="pdxI"
                     /locus_tag="ACHK07_002458"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO8102776.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDILDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            6463..8769
                     /gene="ydbD"
                     /locus_tag="ACHK07_002459"
     CDS             6463..8769
                     /gene="ydbD"
                     /locus_tag="ACHK07_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO8102777.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWGFLESALISWDNLPAVVLKELQQNTSR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            8940..9545
                     /gene="ynbA"
                     /locus_tag="ACHK07_002460"
     CDS             8940..9545
                     /gene="ynbA"
                     /locus_tag="ACHK07_002460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8102778.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            9545..10441
                     /gene="ynbB"
                     /locus_tag="ACHK07_002461"
     CDS             9545..10441
                     /gene="ynbB"
                     /locus_tag="ACHK07_002461"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8102779.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGVLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMV
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            10457..12214
                     /gene="ynbC"
                     /locus_tag="ACHK07_002462"
     CDS             10457..12214
                     /gene="ynbC"
                     /locus_tag="ACHK07_002462"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO8102780.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLIATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            12228..13520
                     /gene="ynbD"
                     /locus_tag="ACHK07_002463"
     CDS             12228..13520
                     /gene="ynbD"
                     /locus_tag="ACHK07_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO8102781.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATIMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(13574..14179)
                     /gene="azoR"
                     /locus_tag="ACHK07_002464"
     CDS             complement(13574..14179)
                     /gene="azoR"
                     /locus_tag="ACHK07_002464"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO8102782.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            14380..18282
                     /gene="hrpA"
                     /locus_tag="ACHK07_002465"
     CDS             14380..18282
                     /gene="hrpA"
                     /locus_tag="ACHK07_002465"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO8102783.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            18554..19354
                     /gene="ydcF"
                     /locus_tag="ACHK07_002466"
     CDS             18554..19354
                     /gene="ydcF"
                     /locus_tag="ACHK07_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO8102784.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTIFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPHVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            19551..20990
                     /gene="aldA"
                     /locus_tag="ACHK07_002467"
     CDS             19551..20990
                     /gene="aldA"
                     /locus_tag="ACHK07_002467"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO8102785.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISSGIRECASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(21032..22033)
                     /gene="gap"
                     /locus_tag="ACHK07_002468"
     CDS             complement(21032..22033)
                     /gene="gap"
                     /locus_tag="ACHK07_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8102786.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            22222..22752
                     /gene="cybB"
                     /locus_tag="ACHK07_002469"
     CDS             22222..22752
                     /gene="cybB"
                     /locus_tag="ACHK07_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO8102787.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            22997..23170
                     /locus_tag="ACHK07_002470"
     CDS             22997..23170
                     /locus_tag="ACHK07_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102788.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            23196..23264
                     /gene="yncP"
                     /locus_tag="ACHK07_002471"
     CDS             23196..23264
                     /gene="yncP"
                     /locus_tag="ACHK07_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO8102789.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(23242..23391)
                     /gene="hokB"
                     /locus_tag="ACHK07_002472"
     CDS             complement(23242..23391)
                     /gene="hokB"
                     /locus_tag="ACHK07_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO8102790.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            23790..25430
                     /gene="trg"
                     /locus_tag="ACHK07_002473"
     CDS             23790..25430
                     /gene="trg"
                     /locus_tag="ACHK07_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO8102791.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVFALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITTVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGKPVSFATV"
     gene            complement(25469..26392)
                     /gene="ydcI"
                     /locus_tag="ACHK07_002474"
     CDS             complement(25469..26392)
                     /gene="ydcI"
                     /locus_tag="ACHK07_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8102792.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            26609..27952
                     /gene="ydcJ"
                     /locus_tag="ACHK07_002475"
     CDS             26609..27952
                     /gene="ydcJ"
                     /locus_tag="ACHK07_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8102793.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            28177..29832
                     /gene="opgD"
                     /locus_tag="ACHK07_002476"
                     /gene_synonym="mdoD"
     CDS             28177..29832
                     /gene="opgD"
                     /locus_tag="ACHK07_002476"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO8102794.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            29972..30196
                     /gene="ydcH"
                     /locus_tag="ACHK07_002477"
     CDS             29972..30196
                     /gene="ydcH"
                     /locus_tag="ACHK07_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO8102795.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            30259..30798
                     /gene="rimL"
                     /locus_tag="ACHK07_002478"
     CDS             30259..30798
                     /gene="rimL"
                     /locus_tag="ACHK07_002478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO8102796.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(30790..31770)
                     /gene="ydcK"
                     /locus_tag="ACHK07_002479"
     CDS             complement(30790..31770)
                     /gene="ydcK"
                     /locus_tag="ACHK07_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO8102797.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGKSR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            31894..32886
                     /gene="tehA"
                     /locus_tag="ACHK07_002480"
     CDS             31894..32886
                     /gene="tehA"
                     /locus_tag="ACHK07_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO8102798.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            32883..33476
                     /gene="tehB"
                     /locus_tag="ACHK07_002481"
     CDS             32883..33476
                     /gene="tehB"
                     /locus_tag="ACHK07_002481"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO8102799.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            33780..34448
                     /gene="ydcL"
                     /locus_tag="ACHK07_002482"
     CDS             33780..34448
                     /gene="ydcL"
                     /locus_tag="ACHK07_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO8102800.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATSKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VDKK"
     gene            complement(34541..34968)
                     /gene="tnpA"
                     /locus_tag="ACHK07_002483"
                     /pseudo
     CDS             complement(34541..34968)
                     /gene="tnpA"
                     /locus_tag="ACHK07_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
     gene            34976..36184
                     /gene="insQ"
                     /locus_tag="ACHK07_002484"
     CDS             34976..36184
                     /gene="insQ"
                     /locus_tag="ACHK07_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8102801.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
BASE COUNT         9224 a         8352 c         9462 g         9168 t
ORIGIN      
        1 gttccaccag atgacggcgg tgatacgccg gttccaccag atgatggcgg tgatacgcca
       61 gttccagacg acggcggtga tacgcctgtt ccgccagacg atggtggcga taccccggtt
      121 aaacacgatc ctgtcgtcta taaaaatgac gtaacctggg atcaggacgc gaaaaccgtc
      181 aaaattcgcg aaaccacctt tacctacagc caaaatgccg atgggagtta caccttaacc
      241 gcgcctgatg gcctgacgac gatagtcaag ggctggacag tccatgacga cagcaacacc
      301 gtcatctttg atggcgtaaa tacggcaggt ggcatcacct ggagctacga cgatgatggg
      361 ctcatccata tcactaaaga ggccggtgtc gttgttgatg gcacaaccgg aactgcgatt
      421 gattttggta atgcgatcat caccgatcag ggcggcaata cggcgctgaa tggcggtact
      481 gtcatgacgg ttgagggtga caacatcacc ctcaacaacg acggtaaaac gaccgccatc
      541 ggcgagggtt cggtcgttgg tatccttact ggtgacgata tcactatcaa taacaacggc
      601 gagacggaag ttgacggcgg taccgcagta atcatcaatg gcgataatgc tacgctgaac
      661 actgcgggcg actcgaccat caccaatggt ggtacgggta gccttatcaa tggcgataat
      721 gcgcgggtcg ataatcaagg taccatgagc gttgacggtg agaattccac tggctcgaaa
      781 attgtcggtg acggtgccac catcaaacag gaaggcgact tatacgtaag cggtggtgcg
      841 cacggtattg acgttgacgg caatgatacg ttcgttagca acaaaggtaa tattacggtc
      901 atcgaagata actctattgg catgctgctt gatggtgatg gcgtcagcgt gatcaacatg
      961 ggcgacctca atgttggtca ggccgctgcg ggtgaaaatg caatcggcat tcagatcgac
     1021 ggtgataatg cgacatttgt taacgttggt gatatttccg cgaccaatgc cggaaccggt
     1081 gtcagcgttg ctggggataa agctaatatt tcactcgcgg gtgggttaga cgttggggat
     1141 ttctccaccg gtcttgatgt ctctggcaat aataacaaca tgacgctggc gacttatgag
     1201 ctgaatgtga cagggcaaaa agcgacgggg gttaatgttt cgggtgatgg caataggatt
     1261 gaaatcgcag gcagcattct ggttgataaa gatcagaaag ccgataatgc gcaaccttac
     1321 ttctttaatc cctcaaccgg cgtaaatatc agcggtaata acaatgacgt cacgctggat
     1381 ggtcagttaa ccgttgttgc tgatagcgaa accacggtgc gttcctatgc aagttacgac
     1441 ggcgctcagg agcacattgc aggtattgtt attgctggcg atgataatac ctttacgctt
     1501 aatggcggtg tccattttgt cggcgaaaaa aatgtcatgg atgatggctc aaaaccatct
     1561 gcttctcgtc gtggtatcgg agacacaccg cttattagcg ttgatgggca ttcatcggtc
     1621 tacctcaatg gtgagtcaac cattagcggc gagttcccgt tgggtttcga aaatctcatt
     1681 cagttgagtc atggcgcgga gctggagatt ggcgctgacg cgacgtttga tatgagtgat
     1741 gtggattcct tcacctatta ttatcgcgtg gcgttatcca ccattagtat tgattctggt
     1801 gcgaaggcga ccaataacgg tgaagttgaa ctgaaaaata tcggttttgc ggctgcctgg
     1861 aataaagatt caacggtcat caataacgga tccattggcc tggcgatgta cgattttggt
     1921 accgacccgg caccaaaagt cttcgatgtt gaatacgggg ggattggcgt taacaatggc
     1981 accttgacca ccaaaatgat gaatcagcac agcgtgctga attatccggc agagtggaac
     2041 ttgtctgacg gtacctcttt caataacaga gcattaggtt tgacaggtat gcttgccagc
     2101 tactcgtcat ccattctgaa cggcgaaacg ggcattattg agatgtatgg tcgtggcagc
     2161 gtggggatgt tggctatcga taaatcaacc gcggataacg aagggcaaat caccctggat
     2221 acgctgtggg tcgatgaaaa tgatgaaaca tcgctgcgta ataacatcgc aaatagcacg
     2281 gcgaaagatt tcggtgtggg aatggcttcg ggaaccgatg cttactatgg agcattaacc
     2341 aaagcgacgt ctaccaacca cgagaatggt gtgattaccg tctacaacgc gggtgcgggg
     2401 atggcggcgt atggtaatac caacacggtc attaaccagg gaacgatcaa ccttgagaaa
     2461 aatgaaaatt acaacgactc gcttggcgta aataaactcg tcggcatggc tgtttacgag
     2521 aaaggaacgg ctatcaatga tcaaaccggt gttattaata ttaatgctga aaatggtcag
     2581 gcattctata acgatggcac tgggcttatt atcaactatg gcaccatttg tacctttggc
     2641 gtttgtcagg gggccgatga gtataatccg acggataact atatcgccga tttgtatgat
     2701 gatggcgatg tattaagctc tgcgggtgag actgtatcat tatcatctgg tgcgattatt
     2761 aatggggatg ttaccagcgc gggtaccgtt aagaatggaa aaatccagat tcttgaaaat
     2821 ggcgcgctgg ttaatgagtc gagcggaacc atcaattcgg ctatcgtcgt taatgcaggc
     2881 ggtacgttaa ccaatgaagg tgtattgaat acggtgacgc tggaaggagg cactttcaat
     2941 aacaacggaa cgcttaatga cgtcgtaaaa attgagaaaa acagcaacgc ggtaattaat
     3001 aataccggtt cactgtcaac cttacagctt aacgatggca cggtgaataa cagcggtatc
     3061 gcgtcggcgc gcgttaacgc ccagggcgat gctgtattca ataaccttgc aggcggcgag
     3121 gcgcgtaaag gtgccattct ctataactct gcggtggtga ataacgcggg gacctggaaa
     3181 atgggttatc aggatgaaaa taacaatgcc gggacgctgg atattgacga ccaatcaacg
     3241 ttcaacaaca gcggcaagct catccttgat aacagtaaaa acgccattcg cttccaggga
     3301 agcaatgcta acgccacgtt atataacacc ggtgaaatga cgctggatgc cgcacttggc
     3361 gcgggcgcta ttcactatga cgacggcgcc agccagttta ttaacaaggg cgtggtggat
     3421 gcgaaagtca ctgtggcggt aagtaccgct ggtgcgacag aaagcgatgc catcctgtgg
     3481 aaccaggatg gcggggtgat taacttcgat aaagacaacg ccagcgcagt taaattcacc
     3541 cacaacaact atgttgccct caacgatggc gtgatgaaca tcagcggcaa caatgccgtg
     3601 gcgatggaag gtgataaaaa cgcacaactg gttaacaacg gcgttatcaa tatcggcacc
     3661 gaaggtacca ccgataccgg gttaattggt atgcaactgg atgccaacgc taccgcagac
     3721 gcggtgatcg agaacaacgg cacgattaat atctatgcta acgattcctt tgcattcagt
     3781 gtgttgggaa cacaaggcca tatcgttaac aacggtaccg tggtgattgc cgatggcgtg
     3841 accggttccg ggttgattaa gcagggcgac agcgtgaacg tggaaggggt gaacggcaac
     3901 aacggtaata ataccgaagt gcattacacc gactacacgt tgccggaagt gccgaacacc
     3961 agttctggtg gcgtaacaac agggggagat gcgactggca gcggtagcaa caacctcaac
     4021 ggttatgtgg tcggtaccaa cgttgacggc agcgccggta agctcaaggt caacaacgcc
     4081 agcatgaacg gtgtcgggat caacaccggt ttcgccgccg gtacggcaga caccacggtc
     4141 agtttcgaca acgtggtgga aggcagcaac ctgaccgacg ccgatgccat cacctcaacg
     4201 tccgtggtgt ggaccgccaa aggcagcacc gatgccagcg gcaacgttga cgtcaccatg
     4261 agcaaaaacg cctacaccga tgtggcgacc gatgcttcgg tgaacgatgt ggcgaaggca
     4321 ctggatgcgg gttacaccaa caacgagctg tataccagcc tgaacgtcgg cactactgcc
     4381 gaactgaaca gcgccctgaa gcaggtgagc ggcagccagg caaccacggt attccgcgag
     4441 gcgcgcgtgc tgagcaaccg cttcagcatg ctggcggacg ccgcgccgaa gatgggcaac
     4501 ggtctggcgt ttaacgtggt ggcgaaaggc gacccgcgtg ctgaactcgg aaataacacc
     4561 gagtacgaca tgctggcgtt gcgtaaaacc gttgacctga gcgaaagcca gagcctgagt
     4621 ctggagtacg gtatcgcccg tctggatggc gacggtgcgc agaaagcggg cgacaacggc
     4681 gtaaccggtg gttacagcca gttcttcggc ctgaagcacc agatgttctt cgacaacggg
     4741 atgaactgga acaacgccct gcgctatgac gtgcataacc tcgacagcag ccgctcggtg
     4801 gcttacggcg acgtcagcaa aacggcagac acggatgtga aacagcagta cctggagttc
     4861 cgcagcgaag gggcaaaaac ctttgaaccg cgcgaagggc tgaaaatcac cccgtacgcc
     4921 ggagtgaaac tgcgtcacac gctggaaggc ggctatcagg agcgcaatgc cggagacttt
     4981 aacctgagca tgaacagcgg cagcgaaacg gcggtggaca gcatcgtcgg gctgaaactg
     5041 gactacgcag ggaaaggcgg ctggagcgcg aatgcgacgc tggaaggcgg gccgaacctg
     5101 agctacagca agagccagcg cacggcaagc cttgcagggg caggcagcca gcactttaac
     5161 gtcgatgacg gtcagaaggg cggcggtatc aacagcctgg cgagcgtcgg cgtgaagtac
     5221 agtagcaaag aaagttcgct gaatctggat gcgtatcact ggaaagagga cggcatcagc
     5281 gacaaaggcg tgatgctgaa ctttaagaaa acgttctaat ttttagcatg tgatacacaa
     5341 accgcaacgc tgatacaggt tgcggttttt tattgccgga tgcgacgctt gacttatctt
     5401 ttgctgtcta atgttttggt gtctttcagg acaatagtgc tactcatcca gaaatcttgt
     5461 ttacgccttc gcattattta tctcttttcg tttctatact gatttttctt aatccgtttt
     5521 attacagggc agggtgcgat gagcagcaat acatttactc tcggtacaaa atccgttaac
     5581 cgtcttggtt atggcgcgat gcaactggca ggtcctgggg tttttggccc cccaagagat
     5641 cgccacgtcg ctataaccgt gctgcgtgag gcgctggcat tgggcgtcaa tcacattgat
     5701 accagcgact tttatggtcc gcacgtcacc aatcagatta tccgcgaagc gctttatcct
     5761 tactctgacg atctgacaat tgtcactaaa attggtgcgc ggcgtggaga ggacgcatcc
     5821 tggttgcccg cattttctcc ggcagagctg caaaaagcgg tacacgataa tctacgtaat
     5881 ctcggcctgg acatactgga tgtggttaac ctgcgcgtga tgatggggga tggtcatggc
     5941 ccggcggaag gatcgattga agccagcctg accgtgctgg cagagatgca acaacaaggc
     6001 ctggtaaaac atattggcct gagcaacgtc acaccgacgc aggttgcaga ggcgcgcaag
     6061 attgccgaaa tcgtctgtgt gcagaacgaa tacaacattg ctcatcgtgc tgatgacgca
     6121 atgattgaag ctttggctcg tgacggcatt gcctacgtgc cgttctttcc gctcgggggc
     6181 tttacgccgc tgcaatcttc cacgctttcc gatgttgctg cgagcctggg tgcaacacca
     6241 atgcaggtgg cgctggcgtg gctgttacaa cgttcaccga atattttgct gatcccaggg
     6301 acgtcttctg ttgcgcattt acgggagaat atggctgctg aaaaattgca tctttctgag
     6361 gaagtgttgt ctacgttgga tggtatttcg cgagaataac gaatatacaa aagggaaaga
     6421 tgcatttccc ttttgtttct tttttaatgg catggagtgc atatgtttaa gcccaggatt
     6481 tgcagctgga taggtttact ccccctgttt atgctgagtc ttccagtaca ggcggaactt
     6541 cgatgtgttg caaatgcagt tgatattgaa ccattttttt ctgcggctac cgctgaagat
     6601 aaacaacaag ttgaacaagc tatcaacagt agcgtgaatc ttgtcccctt cggtttatct
     6661 gcatcggact ggaaagtgca tcgtggcgat ttagtggtag aaggtaatat agagagtaat
     6721 caaaaattga ttgttcttgg caatttgaca gtcaaaggta atatttccac tttctctctt
     6781 tctaatccat gggttattct cggtaacgtg acggcaacga atatagttac ggattcacca
     6841 ctgttaatta ccggttcgat aaatgcaagt ggactggtat ttatcgactc atattacgat
     6901 aatccgtcta cgattaaggg gagtattaat gcgcgtggga tatttatcaa tgacataatt
     6961 gcacctgttg ttgcgtcttc gacaaatagt gaattcatgg tccgtgcgag tgataaaaat
     7021 gacactgaaa atgtcaaaaa agcgttgaag ataataaatc ctgatgcata ttattggggt
     7081 ctaataaatg atgaagatgc tctgaaagaa atttttaagc gaagcaatat tcgcatggca
     7141 gggaatgtct gtaatcagat gaaaaaagaa gcgctgtttc gccctaagcc ttctcctgag
     7201 ttagtgcagg aattgcaaat gctggatgaa ggcaacgttg ctgcatttga aggacgagat
     7261 attgcgacat ttgatcttgc cataatgcga actctcccaa ggcttaaagg aatttcagct
     7321 aacctacgca aacaacttat taatagcaat gatggacaaa cgattgcaag tatggcgaga
     7381 tatatgcctg acaatgagat tctggagttg accgatcagc aattgggtta ccagcctgtt
     7441 gttctggggt tgctcgaccg tgaaccgctc tctgtcgaaa taatgacgcg aatgagtcat
     7501 ctacctgatg gtgtcggtcc gttgaatctt gcgctacgtg aaaatctccc tctggatatc
     7561 gtcatgactc tggcgaaaag agattgggat atgatcattc aggagcttta taaagatgcg
     7621 tggttattgc ccgaatccat tattgatggc tatatccgca gtgatgattc ctctattcgt
     7681 caggtcggtg ctggaggaca actcacctac aatcaggcaa tgcagttggc gaacgattca
     7741 tcaaacgatg ttgtcacaag cttagcgtta aagctggcag agatgaaaca ccatggtcaa
     7801 ttgttgcgga tgacgccaca agagagtgac aaaatagcag tatatttata ccaaaaattc
     7861 gagaatgacg atgatctaat aggtgcgtta tttttagcgt tgccagataa cttacagttt
     7921 aattttgtta aaaggatgga gaaaaaatcc cctgcctact tttgctgtcg ggatatgcag
     7981 ataattcact ctgacgctgc tttacaacgg ttattgacac gtttcaacga tcctgagggg
     8041 tggagtaatc tggcgaaaaa tcagtatctg agcacgtcga tgaaacaaaa aatttggcaa
     8101 cgtgctttat cgcatcggaa aaataatccc aaagcagatt cagatgcata tgaaaccagc
     8161 gctgatatga ttttgtctga gcttatcagc tacggtgaag ttgatgacca gatgctatta
     8221 aatgccacct ctctgatacg ttcggacgac tggggctttt tagagagtgc attaattagt
     8281 tgggataatt tacccgctgt tgttctcaag gaattacagc aaaacacatc acgcaatgat
     8341 atttgggcga agttttttct gaggcaggaa aacagttccc gtgcgcaggt taacgaagcg
     8401 ttacgtgttt attatgcact agaccccgat gcgttagcac aactggatgt actggcaaaa
     8461 caaccggatc gtatatggtg gagtacactg gcgaaaagca atctcacatt tttcaagttc
     8521 ggcgcactta acaaccgcca cacaccgcct gcagtactgg cggcagaaat tgatcccgag
     8581 tggtggattg tggcgatgaa taatccccgt tttccagttg atgtattaaa ggcgaggctg
     8641 aaacgtgatc ctttgctggc gttagagctt gttaaccctg aactggattt agttcgccag
     8701 ctggcgctta acggtaagac gcgcgcaata cgggaacaag cgatgagaaa acttgatgag
     8761 ttgtattgaa taacagcgta ataattttta accacttgat ttattgggga taatcattcc
     8821 tgacagtgag tccccaatac cttgatatat tctgaatttt taatgaagcg tcgtgttgcg
     8881 atatctccgt caggggaatt gatgcaccat agcgcaaacc gaattatcaa ggattgataa
     8941 tgacgctcta ccagataaaa ccgctctttc agtcgctgtt aaggccgacg atgttttggc
     9001 tttataagca ccatgttaca gcgaatcaca tcactcttgc tgcactggcg ctttctctta
     9061 ttaccggatt gctgttgatg ttggtcgcac aacccatcct ctttttgcta ttgcccatcg
     9121 tgctttttat ccgtatggca ctcaatgcgc tggatggcat gttggcgcgt gagtgcaatc
     9181 agcaaacacg tctgggggcg attttgaatg aaactggcga tgttatttcc gatattgcgc
     9241 tctatttacc gtttttattt ttaccagaaa gtaacgcatc actcgtaata ctcatgttat
     9301 tttgcaccat attgactgag ttttgcggtt tactcgcgca gacgattaac ggtgttcgca
     9361 gttatgtcgg tccttttggc aaaagcgatc gcgcgttaat atttgggttg tgggggctgg
     9421 ccgttgccat ttatccacag tggatgcaat ggaataatct tttatggagc attgcttcaa
     9481 ttctgcttct ctggactgcg attaatcgtt gtcggagtgt gctcctgatg agtgctgaaa
     9541 gataatgctg gaaaaatctc tggcaacact tttcgcgttg ttaattttag caacgctgat
     9601 aaaccgtttt cgggtatggc ggttaccgga gagaaaaggg gatgaagtta cattacgtat
     9661 tcgtacctgg tggggcatcg tcatttgttt ttcactggtg atttcaggtc cacgctggat
     9721 gacgttgacg ttttttgcgc tgataagttt tctggcattg aaagaatatt gtacgcttat
     9781 atccgtacat tttccgcgtt ggttatattg ggttattcct cttaactatt tgcttatcgg
     9841 ttttaattgc tttgagctgt ttctgttatt tatacctttg gctggggttc tgatattaac
     9901 cacctggcga gttttagtag gtgatacctc cggttttctg cataccgtga gcgccatttt
     9961 ttggggctgg ataatgaccg ttttcgcctt gagtcatgcc gcctggttat taatgttgcc
    10021 aaccacaaat acccagggcg gggcgttact ggtgttattt cttttggcgt taacagagtc
    10081 aaacgatatt gcacagtatt tatgggggaa atcctgcggc agaagaaaag tggtccccaa
    10141 agtcagcccg ggaaaaacgc tggaaggtct ggtggggggc gtaatcacca ccatggttgc
    10201 gtcactgatt attgggccgt tgctgacacc gctaaataca ttacaggcat tattagcggg
    10261 tttgttaatt ggtattagtg gtttttgcgg cgatgtagtg atgtcagcca tcaaacgaga
    10321 tattggtgtt aaagatagcg gaaaactatt accaggacat ggcggactcc ttgacaggat
    10381 tgactcatta attttcaccg ctccggtatt tttttatttt attcgctact gctgttactg
    10441 aaggaaaata gagaaaatgg aaaattcacg catccctggg gaacattttt ttaccaccag
    10501 tgataataca gcgttgtttt atcggcactg gcccgcttta cagcccgggg cgaaaaaggt
    10561 catcgtctta tttcatcgcg gtcatgaaca ttctggtcgt ctacaacatc tcgttgatga
    10621 actggcgatg ccagatactg ctttttatgc atgggatgcc cgagggcatg gaaaaacttc
    10681 ggggccgcgt ggttatagcc catctcttgc gcgttcagtg cgggatgtcg atgaatttgt
    10741 ccgttttgct gccagcgaca gccaggttgg actggaagag gtggtagtga tcgcgcaaag
    10801 cgtcggcgca gtgctgattg ccacatgggt tcatgattat gcgcctgcaa ttcgcgggct
    10861 ggtgctggct tctccggcct ttaaggttaa attgtatgtg ccgctggcac gtcctgcgct
    10921 ggcgttatgg catcgtctgc gtggtctgtt ttttattaat tcctatgtga aaggacgcta
    10981 tttgacccac gatcggcaac gggtggcgag tttcaataat gatccgctga tcacaagggc
    11041 gattgccgtc aatatcttgc tagatctcta taaaacgtct gaacgtattg ttagcgatgc
    11101 ggcggcgatt acgctcccca cgcaacttct gatatcaggc gatgactatg tggtgcatcg
    11161 ccaaccgcag attgattttt atcagagatt acgtagccct ctgaaagagc tgcatctgct
    11221 gccaggcttt tatcacgaca cgttgggtga agagaacagg gcgcaggcat ttgaaaaaat
    11281 gcaaagcttt attagtcgtt tatatgctaa caagtcacaa aaatttgatt atcagcatga
    11341 agaccgcact ggaccatcag cggatcgctg gcggctcctt tcaggtggac ccgtgccatt
    11401 atcgccggtt gatttggcgt atcgctttat gcgtaaagcg atgaaattgt tcgggacgca
    11461 ctctgcgggc ctgcatctcg gaatgagcac cggctttgat tcaggcagtt cgctggatta
    11521 tgtctatcaa aatcaaccgc aaggtagtaa cgcattcggg cgcttaatcg acaaaatcta
    11581 cctgaacagt gttggctggc gcggtattcg ccagcgcaaa acccatttac aaatactgat
    11641 taaacaagcc gttgccgatc tccacgccaa aggtttagcc gtccgcgtgg ttgacattgc
    11701 cgcagggcat gggcgctatg tactggatgc gctggcaaac gaacctgccg taagcgatat
    11761 tttgttacgt gattacagtg agttaaatgt tgctcagggg caagagatga ttgcccaacg
    11821 gggaatgtct gggcgggtgc gttttgaaca gggcgatgcg tttaacccgg aggaactcag
    11881 cgcgttaact ccgcggccta cgctggcgat tgtttctgga ctgtatgagc tttttcccgg
    11941 aaatgagcag gtaaaaaact cactcgcagg tcttgccaat gccatcgaac cgggtggcat
    12001 tctcatctac accgggcagc cgtggcaccc acaactggag ctgattgccg gggtgttaac
    12061 cagtcataaa gatggtaaac cgtgggtaat gcgcgtgcgt tcgcaagggg agatggattc
    12121 gctcgtgcgt aatgccggat ttgataaatg cactcaacgg attgatgagt ggggcatttt
    12181 tacggtttcg atggcggtgc gtcgtgataa ctgaacgccg gaacgtattg ctacaaggcg
    12241 ctggctggtt attgttgctg gccccgtttt tcttcttcac ctatggatct cttaatcagt
    12301 tcaccgcggt tcaggacttt aacagccatg atatccccag tcaggtattc ggctgggaaa
    12361 cggcgatccc ttttctcccc tggactattg ttccttactg gagtctggat cttttatatg
    12421 ggttttcgtt gttcgtttgt agttcgacat tcgaacagcg ccgactggtt caccggctta
    12481 ttctggcaac gataatggcc tgctgcggtt tttttctcta tccgctgaag tttagtttta
    12541 tccgtcctga agtgagtggg gtgacaggat ggctattttc gcaacttgaa ctgtttgatc
    12601 tgccttataa ccagtctcct tcgctgcata ttattctctg ctggctactt tggcgtcact
    12661 ttcgtcagca tctggctgtg aggtggcgta aagtctgcgg cggatggttt ttactcatcg
    12721 ccatttcgac gctgacaacc tggcagcatc attttattga tgtcatcaca gggctggcgg
    12781 taggtatgtt aattgactgg atggtgcccg tcgaccgtcg ttggaattat cagaaacctg
    12841 atcaacgtcg aatcaaaata gcactgccat atgtcgtagg cgcgtgctcg tgcattgtgt
    12901 tgatggagct aatgataatg cttcagttat ggtggtcagt ctggttatgt tggccagtat
    12961 tatcgctatt catcattggc cgtgggtacg gtgggcttgg cgcgataaca acagggaaag
    13021 atagtcaggg gaaactcccg cccgccgttt actggctgac attgccctgg cgcatcggga
    13081 tgtggctgtc tatgcgttgg ttttgtcttc gcctggagcc ggtgagcaaa atgactgctg
    13141 gtgtttattt aggggcgttt ccacgacata ttccggcaca gaatgcggtt ctggacgtca
    13201 cctttgaatt ccctcgcgga cgagccacaa aagatcgact ctatttttgt gtaccgatgc
    13261 tggatctggt ggttccggaa gagggggagc tccgacaggc cgtggcgatg ctggaaacat
    13321 tacgcgaaga gcaaggcagc gttctggtcc attgtgcatt gggattatcg cgcagtgcgc
    13381 tggtggtggc ggcatggttg ttatgttacg gacactgtaa aaccgttaat gaagcgatta
    13441 gctatattcg agccagacgc ccgcagattg tgctgacaga cgaacacaaa gcaatgctga
    13501 gattgtggga aaacaggtaa gtggattgag atgtggactg aatatctaca gtccacatca
    13561 agaccgtgtc cggttatgca gcaacaatgc tgtcgatggc tgctttcgcg tcagactgcg
    13621 ctttcgctgc catttccgga ccgtatgcga tcccttcggc gaagacaaat ttcacatcag
    13681 taatgccgat aaagcccagg aacgtggaca gatacggcgt caccaggtcc gttggtccat
    13741 ctttgtggat cccaccgcgg ctggtaataa cgatggcttt tttacccgtt accagacctt
    13801 ccggaccatt ctcggtatag cggaaagtaa cgcctgcacg tgcaaccagg tcaaaatagt
    13861 ttttcagctg agtcgagatg ttgaagttat acatcggtgc cgcaataacg ataacgtcat
    13921 gggctttcag ctcggcaatc agctcatcgg aaagtgccag agcttcctgc tgacgcggag
    13981 tcagcggcgc atcgctcgga cgcagcgcac caaccagttc gccatccagt accggaatcg
    14041 gatttgcagc caggtcgcga acggtgattt catcagcgga gtgcttttcg cgccattgtt
    14101 caacaaaata atcggacaac tgattagact gagagtaccc tgccaggatg ctggatttaa
    14161 gaactaatac cttgctcatg gtgtttcctt atagatgttt gaatgggcga tgccccgttg
    14221 cttgctgaca ctttattcac aatcctgcca cagagatagc gcaataaatc gaagcctatg
    14281 ttcgaattta ttgaacaacg catagaaagc cgcgatgtgg tactctatat ctatcattta
    14341 aaagaaaatt aatcaggcag cctactgccc tctaacgtta tgacagaaca acaaaaattg
    14401 acctttacag ccttgcagca gcggctggat tcgctgatgc tgcgtgacag actgcgtttt
    14461 tctcgccgtc tgcatggtgt gaagaaggtt aaaaatcctg atgcacaaca ggccattttc
    14521 caggagatgg cgaaagagat tgaccaggcg gcagggaaag tcctgctgcg tgaagcggca
    14581 cgaccggaaa ttacttaccc ggacaattta ccagttagtc agaaaaagca ggacattctc
    14641 gaagcgattc gtgatcacca ggtggtgatc gtcgccgggg aaaccggttc cggtaaaacg
    14701 actcagctac cgaaaatctg tatggagctg gggcgcggta ttaaagggct gatcggtcat
    14761 acccagccgc gtcgtctggc ggcaagaaca gtggcaaacc gtattgcgga agagctgaaa
    14821 acggagccgg gcggttgcat cggttacaaa gtgcgtttca gcgatcacgt aagtgataac
    14881 acgatggtca agctgatgac cgacggtatc ctgctggcgg agatccagca agaccgcctg
    14941 ctgatgcagt acgacactat cattattgac gaagcgcacg aacgcagcct gaatatcgat
    15001 tttttgctcg gctatttgaa agagttgcta ccgcggcgtc ctgacctaaa aatcattatc
    15061 acttccgcga ctatcgaccc ggaacgcttt tcgcgccact ttaataatgc gccgattatc
    15121 gaagtctccg gtcggaccta tccggtggaa gtgcgctatc gcccgattgt tgaagaagcc
    15181 gatgacaccg agcgcgatca attgcaggcg atttttgacg ccgtagacga actgagccag
    15241 gaaagccccg gcgacattct gatctttatg agcggcgagc gggaaattcg cgataccgct
    15301 gatgcactga acaaactgaa cttacgacat acggaaatct tgccgcttta tgcgcggctt
    15361 tcgaatagcg aacagaatag ggtgttccag tcgcatagcg gacggcgcat tgtgctggcg
    15421 accaacgtcg cggaaacgtc gctgaccgta ccggggatta aatacgttat cgaccccggt
    15481 acagcgcgta tcagccgcta cagctatcgc accaaagtgc agcgtttgcc gattgagccc
    15541 atttcgcagg cgtctgccaa tcagcgtaaa ggccgctgtg gtcgtgtgtc cgaagggatc
    15601 tgtattcgtc tctattccga agacgatttc ctctcgcgcc cggagtttac cgatccggag
    15661 attctgcgta ccaacctggc ctcggttatt ttgcagatga ccgcgctggg gctgggcgat
    15721 atcgctgcgt tcccgtttgt cgaagcaccg gataaacgca atatccagga tggcgtgcgt
    15781 ctgctcgaag aactgggcgc gatcaccacg gatgaacagg ccagcgccta taaactgacg
    15841 ccgctcggtc gccagctctc gcagttgcct gtcgacccac gtctggcgcg tatggtgctg
    15901 gaagcgcaaa aacatggctg cgtgcgtgag gcgatgatta tcacgtctgc gctctccatt
    15961 caggatccgc gcgaacgtcc gatggacaaa cagcaggcat cggacgaaaa acatcgtcgc
    16021 ttccacgaca aagagtctga cttcctcgcg tttgtgaatc tgtggaatta tcttggcgag
    16081 cagcaaaagg cgctttcttc caacgccttc cgtcgcctgt gtcgtaccga ttatctcaac
    16141 tatctgcgtg tgcgcgaatg gcaggatatc tacacccagt tgcgccaggt ggtgaaagaa
    16201 ctcggcattc cggtgaacag cgaaccggcg gagtatcgtg aaattcacat tgcgttgctg
    16261 acgggtttgc tttcccatat cggcatgaaa gatgccgata aacaagaata taccggcgca
    16321 cgtaacgcgc gtttctccat cttccccggt tctggtttat tcaaaaaacc gccgaaatgg
    16381 gtaatggtgg cggaactggt agaaaccagc cgcctgtggg ggcgcattgc tgcgcgtatc
    16441 gacccggaat gggtggagcc tgtcgctcag catttgatta aacgtaccta cagcgaaccg
    16501 cactgggaac gggcgcaggg cgcggtgatg gcaacggaaa aagtcactgt ttatggtttg
    16561 ccgattgttg ccgcgcgcaa ggtcaactac agccagatcg atccggcgtt atgtcgtgaa
    16621 ctctttattc gccacgcgct ggtggaaggt gactggcaga cgcgtcacgc attcttccgt
    16681 gaaaacctga aactgcgggc cgaagtggaa gagctggaac acaaatcacg tcgccgcgat
    16741 attctggttg atgacgaaac gttgtttgag ttctacgacc agcgcatcag ccacgatgta
    16801 atctccgctc gtcacttcga cagttggtgg aaaaaagtca gccgcgaaac gcctgatttg
    16861 ctcaactttg aaaagagcat gttgatcaaa gaaggggcgg aaaagatcag caagctggat
    16921 tatccgaact tctggcatca gggcaatctc aagctgcgtt tgagctatca gtttgagccc
    16981 ggcgcggatg ctgacggtgt gaccgtacat attccgctgc cgttacttaa ccaggttgag
    17041 gaaagcgggt ttgaatggca gatccccggt ctgcgccgcg aactggtgat tgctctgatt
    17101 aaatcgttgc cgaaaccggt acgccgtaat tttgtacccg cgccaaacta cgccgaagcg
    17161 tttttaggcc gcgtcacacc gctggagtta ccgttgcttg acagccttga gcgcgagtta
    17221 cggcggatga ccggcgttac cgttgaccgc gaagactggc actgggatca ggtgcccgat
    17281 cacctgaaaa tcaccttccg cgtggtggat gacaaaaaca agaagctaaa agaagggcgc
    17341 tcgctacaag atctgaaaga tgcgctgaaa ggcaaagtgc aggaaacgct gtctgcggtg
    17401 gcggatgacg gtatcgagca gagcggctta catatctgga gttttgggca actgccggaa
    17461 agctacgagc agaagcgcgg caactacaaa gtgaaggcgt ggccggcgct ggtggatgag
    17521 cgcgacagtg tggcgatcaa actgtttgat aacccgctgg agcaaaaaca ggcaatgtgg
    17581 aacggtcttc gccgtctact gctgctgaat attccatcgc cgatcaagta tttacatgag
    17641 aagttaccaa acaaagccaa gcttggactg tactttaacc cgtatggcaa agtgctggag
    17701 ctgatcgacg actgtatctc ctgcggtgtg gataaattga tcgacgccaa tggtggcccg
    17761 gtctggacgg aagaaggctt tgctgcgctg catgaaaaag tgcgtgccga actgaacgac
    17821 acggtggtgg atattgcgaa gcaggtcgag caaatcctta cggcagtgtt caatatcaac
    17881 aagcgtctga aagggcgggt ggatatgacc atggcgctgg ggctttctga cattaaagcg
    17941 cagatgggcg ggttggtata tcgcggtttt gtcactggta acggcttcaa acggctgggc
    18001 gacacgctgc gttatttgca ggcgattgaa aaacggctgg aaaagctggc ggttgatcca
    18061 catcgcgatc gtgcacagat gctgaaagtc gaaaacgtcc agcaggcgtg gcagcaatgg
    18121 atcaacaaac tgccgcctgc acgtcgtgag gatgaagacg tgaaagagat ccgttggatg
    18181 atagaagagc tgcgcgttag ttacttcgct caacaacttg gtacgcctta tccgatttca
    18241 gataagcgta ttttgcaggc gatggagcag attagcggtt aacgctgcta tttgcctgat
    18301 aaatataaaa cccggtaagc atttagcgcc gggtttttta ttaattctaa aacggtaagg
    18361 gcaaaaattc aggaattcag aaaaatacaa ttctctgctg caagatgaat aatgtttatc
    18421 tacagcattt ccttaaaaga tatgtcaggc ttgcggagtg gcggttaagg aaatacgaca
    18481 tcctcctttc agagtgctcc gcttctcact attatctcac gcagtattct taagggaacg
    18541 ataaggagga accatgaaca ttaccccgtt tccgacgctt tcgacggcaa ctatagatgc
    18601 cataaatgtt atcggacagt ggctggcgca ggatgatttc tccggtgagg tgccgtatca
    18661 ggccgattgc gtcatacttg caggcaatgc ggttatgccg actatagatg cggcatgtaa
    18721 gattgcccgc gatcagcaaa ttcctttact gattagtggt ggtatcggtc actcgaccat
    18781 ttttttgtat agcgccatcg cacagcatcc gcactacaac actatccgca ccactggcag
    18841 agcagaagcg accatcctgg cggatatcgc tcatcagttc tggcatattc cgcatgaaaa
    18901 aatctggatt gaagaccagt caacaaactg cggtgaaaac gcacgcttta gcatcgcgct
    18961 attgaatcag gccgtagaac gagttcatac ggctatcgtt gtacaggacc ccaccatgca
    19021 gcggcgcacg atggcaacgt tccgccgtat gaccggggac aatccccatg taccacgctg
    19081 gttaagttat cccggattcg ttcctcagtt aggaaataac gcagacagtg taatctttat
    19141 taatcagtta caaggattat ggccggttga gcgttatctc tcactactca ctggcgagct
    19201 gccgcgttta cgcgatgata gcgatggcta cggtccccgc gggcgagatt ttatcgttca
    19261 cgttgatttt ccggcagaag tcattcaggc atggcaaacg cttaaacacg atgcggtgct
    19321 catcgaggcg atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg aaccacttgt
    19381 ttgcaaacgg gcataactcc tgccttttat ttctgcctct tattcctttt acacttgttt
    19441 ttatgaagcc cttcacagaa ttgtcctttc acgatgccgt ctctctgatg attgatgtta
    19501 attaacaatg tatttaccaa aaacaaacat ataaatcaca ggagtcgccc atgtcagtac
    19561 ccgttcaaca tcctatgtat attgatggac agtttgttac ctggcgtgga gacgcatgga
    19621 ttgatgtggt aaaccctgct acagaggctg tcatttcccg cattcccgat ggtcaggccg
    19681 aggatgctcg taaggcaatc gatgcagcag aacgtgcaca accagaatgg gaagcgttgc
    19741 ctgccattga acgcgccagt tggttgcgca aaatctcctc cgggatccgc gaatgcgcca
    19801 gtgaaatcag tgcgctgatt gttgaagaag ggggcaagat ccagcagctg gctgaagtcg
    19861 aagtggcttt tactgcggac tatatcgatt acatggcgga gtgggcacgg cgttacgagg
    19921 gcgagattat tcaaagcgat cgtccaggag aaaatattct tctgtttaaa cgtgcacttg
    19981 gcgtgactac tggcattctg ccgtggaact tcccgttctt cctcattgcc cgcaaaatgg
    20041 ctcccgctct tttgaccggt aataccatcg tcattaaacc cagtgaattt acgccaaaca
    20101 atgcgattgc attcgccaaa atcgtcgatg aaataggcct tccgcgcggc gtgtttaacc
    20161 ttgttctggg gcgtggtgaa accgttgggc aagaactggc gggtaaccca aaggtcgcaa
    20221 tggtcagtat gacaggcagc gtctctgcag gtgagaagat catggcgact gcggcgaaaa
    20281 acatcaccaa agtgtgcctg gaactggggg gtaaagcacc agctatcgta atggacgatg
    20341 ccgatcttga actggcagtc aaagccatcg ttgattcacg cgtcattaat agtgggcaag
    20401 tgtgtaactg tgcagaacgt gtttatgtac agaaaggcat ttatgatcag ttcgtcaatc
    20461 ggctgggtga agcgatgcag gcggttcaat ttggtaaccc cgctgaacgc aacgacattg
    20521 cgatggggcc gttgattaac gccgcggcgc tggaaagggt cgagcaaaaa gtggcgcgcg
    20581 cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg aaaggatatt
    20641 attatccgcc gacattgctg ctggatgttc gccaggaaat gtcgattatg catgaggaaa
    20701 cctttggccc ggtgctgcca gttgtcgcat tcgacacgct ggaagatgct atctcaatgg
    20761 ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg aacgtcgcga
    20821 tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa aacttcgaag
    20881 ctatgcaagg cttccacgcc ggatggcgta aatccggtat tggcggcgca gatggtaaac
    20941 atggcctgca tgaatatctg cagacccagg tggtttattt acagtcttaa tgagtgaaag
    21001 aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt tcgagggtgc
    21061 gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt ttcaccagtt
    21121 gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa ccgaaatggc
    21181 tgccaatgat atcggaagag actatttctt catcggtata accaaatgac tcgttattgg
    21241 tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttt ccgagaatcg
    21301 ataccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga cctttcagtt
    21361 tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcgcccgta gtgtggggaa
    21421 tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgcggg ccatccacca
    21481 gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac
    21541 tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt ggtgcatgac gccacggaaa
    21601 caatggtatc gttgccatcc agagtgtcgt cattaacgtt ataaacgatg gttttcattt
    21661 caccggcagg agcggaaatc aacaccttct tcgcaccagc atcaagatgc gcctgcgatt
    21721 tctcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcacct ttcgctttcc
    21781 acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca ttaacgataa
    21841 gtgaatcttc cgtaaaatca acgctccagg ggaacggtcc gtagtttgaa tcatgtttca
    21901 gcaggtaggc gagaattttt ggagaagtga gatcattaat agcgacaacg tctatgttgc
    21961 ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac
    22021 caactttact catggttttc tcctgtcagg aacgttcgga tgaaaattga tcctttccag
    22081 gcttagacca ggatggcggg atgggcaatc tccattctta cagtgaaacg taacgtactg
    22141 aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat
    22201 tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat cagcattcac
    22261 tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg gggtttcttc
    22321 ccacgtagcg atcggccact catcaacatg attcatgttt cctgcggcat atcaattctc
    22381 gtgctgatgg tcgttcgtct tttgttaagg ctgaaatacc caaccccgcc gattatacct
    22441 aagccaaaac cgatgatgac gggactggcg catttgggac atttggtgat ttatctcctg
    22501 tttattgcgc tgccagtgat tggtttggtg atgatgtata accgaggcaa cccgtggttt
    22561 gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt agatagctta
    22621 aagtcgtggc atgaaacgct ggcgaatctg ggatattttg tcatcggttt gcacgctgcg
    22681 gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat gatgccgcgt
    22741 aaacgttcct gaaggatatt taaagaaaac gcctgtacta aaaccgaccc gtggtacagg
    22801 cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga caaataaaac
    22861 cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc gatctttgat
    22921 ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat aagattcgat
    22981 aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa cgcttgtttc
    23041 cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga tctcacactg
    23101 tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga aaaaaacatg
    23161 cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg
    23221 ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag caacctcctt
    23281 atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag
    23341 aatcgtaatg cagataatga gcagacacac caccagaggg ttgtgcttca tagcctttct
    23401 ccttgccgga tggcgggtaa gaggctaaga tctgaattgc taggttcatt cgttggcctc
    23461 ggttgataga aatatcggtc ggggccttcg tctttctgat tcccggttag cctgaaaaca
    23521 gaaagtctca ggcacccgtg agcatcctat gtggtttcct tagggacgaa aataatcacc
    23581 tgacgaaatt tcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc agtttaacca
    23641 ttcgttactc tcttcattcc aatggcatta atcttctctg caataattgt tgtaaaaatg
    23701 tgacgcaaag aggcttttag tcgtaagtaa ttaccgtcaa atgccgatga ctttctatca
    23761 ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacag cgattaggtt
    23821 ttttgcatca catcaggttg gttccgttat ttgcctgcat tctaggcggt atcttagttc
    23881 tattcgcatt aagttcagcc ctggctggct atttcctctg gcaggccgat cgcgatcagc
    23941 gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca gattttttgc
    24001 gttcagcccg gatcaatatg attcaggccg gggctgcgag tcgtattgcg gaaatggaag
    24061 caatgaagcg aaatattgcg caagccgagt cggagattaa acagtcgcag caaggttatc
    24121 gtgcttatca gaatcggccg gtgaaaacac ctgctgatga agccctcgac actgaattaa
    24181 atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat gccaaaaatg
    24241 gtatgtttga agcaattatc aatcatgaaa gtgagcagat ccgaacgctg gataatgctt
    24301 ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagagccaac caactggcgg
    24361 aactggccca tcagcgcacc cgcgtgggtg ggatgtttat gattggcgcg tttgtgtttg
    24421 ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt cgtccactgc
    24481 aacatgccgc acaacggatt gaaaaaatcg ccagtggcga tctgacgatg aatgatgaac
    24541 cggcgggtcg taatgaaatc ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac
    24601 tggggatgac agtagggact gttcgacagg gcgcggaaga gatttatcgt ggcaccagcg
    24661 aaatttccgc tggcaatgcg gacctttcat ctcgcaccga agaacaagcg gcggcaattg
    24721 aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat gcggataacg
    24781 cgcattatgc cagtaaactg gcgcaagagg cttctattaa agccagcgat ggcgggcaga
    24841 cggtttccgg tgtagtaaaa acgatgggcg ctatctccac gagttcgaag aaaatttctg
    24901 agatcaccac cgtcatcaac agtattgctt tccagacgaa tattctggca ctgaatgctg
    24961 ccgttgaagc tgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag
    25021 tacggacact cgcaagccgc agcgcccagg cggcgaaaga gattgaaggc ttgatcagtg
    25081 aatcagtcag gttaattgac ctgggatcgg atgaggtggc aacggccggg aaaaccatga
    25141 gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc gccgccgcct
    25201 cggatgagca aagtagaggc ataactcagg ttagccaggc gatttctgaa atggataagg
    25261 tgacgcaaca gaatgcttct ctggtagaag aggcctcagc ggcggcggtg tcccttgaag
    25321 aacaggcggc acgattaact gaggcggtgg acgtattccg tctgaacaaa cattctgtgt
    25381 tggcagaacc tcgcggagcg ggtaaaccag ttagtttcgc tacggtgtga aaatgttcaa
    25441 ggaagggatc gacagatccc ttcacctttc agaacggcat tgattttcga atagcgttaa
    25501 tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt ccaatcggtt
    25561 cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc aggtcgtctt
    25621 ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt tgacgggata
    25681 gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acatccctgg ctctgtacta
    25741 atgcatctga atgctggcgt ggtgcagtgc cttctggtga tacaacgacc ggccattcca
    25801 gcactcggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg acaaccagct
    25861 tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca ggatctgaca
    25921 tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc atagggttac
    25981 tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga cctataaccg
    26041 aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca tcattattaa
    26101 gaccttcttt acgatgaagc gactgtccag cagtgttgat ggcgtcaaga actctgactg
    26161 catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga ccacgctcaa
    26221 acaagcgagc gcccgtcagt tgctccagtt cattcaatgt cttagagagc gcaggttgac
    26281 tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca gctacgaatg
    26341 tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc ataagcgatg
    26401 ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa cttgattgca
    26461 aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat tgatgttttc
    26521 gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta cctacactcc
    26581 agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg atgagattcg
    26641 ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc aatatggcac
    26701 gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc ctcaactgca
    26761 cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc atggggcgat
    26821 tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca ttatggggat
    26881 gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt cgacagcatt
    26941 tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta cctccttact
    27001 ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga ttctgcgtca
    27061 gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc agcagggcgg
    27121 ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt ttcgctggca
    27181 ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac atcggttgat
    27241 tgctgatgtg gtctgtttcc ctggatgcca tatcaaccac ctgacgccac gtacgctgga
    27301 tattgaccgg gtgcagtcga tgatgcctga atgcgggatt gaacccaaaa ttctgatcga
    27361 agggccgccg cgccgcgagg tgccgatttt actacggcag accagcttta aggcactgga
    27421 agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct ttggtgaaat
    27481 tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg atcttctgcg
    27541 taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg aaaccttccg
    27601 cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ctggcatggt tccggtaccg
    27661 tctgacgcct tcaggtgagg cgcatcgtca ggctattcat cccggagacg atccacagcc
    27721 cttaattgaa cgtggttggg tcgcggcgca acccattacc tatgaagatt tcttgcccgt
    27781 tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcaggcac gcagccacgg
    27841 taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg atgagttcca
    27901 gctttatcag gaagcggaag agcgcagtaa acgtcgctgt ggtttgcttt aaattctgac
    27961 catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat cccatcgaca
    28021 taaaaaaatt gccgatttat gcatattctc tgagttcaac aattggatta ttaataaata
    28081 ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg accagcgcgg
    28141 gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg atttattaaa
    28201 ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct tttttctcag
    28261 gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt tgacttctcc
    28321 attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc gcctcgtccg
    28381 ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca atacgacgcc
    28441 gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt cttccatatg
    28501 ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac acatctggcg
    28561 cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt tgataccaaa
    28621 caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa agcccccgaa
    28681 ctggcgcgtc gtgatgtagt atcattcctc ggtgcgagtt atttccgcgc cgttgatgac
    28741 acatatcaat acggtttgtc ggctcgcggc ctggcgatcg acacttacac cgacagtaaa
    28801 gaagaattcc ccgactttac cgccttctgg tttgatacgg taaaaccggg ggcaactacc
    28861 tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa gttcactatc
    28921 cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc gcgcaaagac
    28981 attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac taatgaacgt
    29041 cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc catgtggcgg
    29101 ggcaacggcg agtggatttg ccgtccgctg aataatccgc aaaaattgca gttcaatgct
    29161 tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga cttctcccat
    29221 tatcaggaca tcatgggctg gtataacaaa cgcccaagtc tgtgggtgga accgcgtaac
    29281 aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga aacgctggat
    29341 aacattgtct gcttctggca gccagaaaaa gctgtaaaag cgggtgatga gtttgcattc
    29401 cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc gcgcgttatg
    29461 gcgacgcgta ccggcatggg cggtttcccg gaaggttggg caccaggtga acactatccc
    29521 gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa agctgccgca
    29581 ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca aatcgaaatt
    29641 ctctatattg aacccattga tggttatcgt attcagtttg actggtatcc gacttcggac
    29701 tccactgatc cggtcgatat gcggatgtat ctgcgttgtc agggggacgc tatcagtgaa
    29761 acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt tgacgaccgc
    29821 gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc tggttaaaca
    29881 cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg tatttcagat
    29941 acaccttgtc agccactaac agggagtgcg tatgtttcca gaatatcgag atttaatatc
    30001 ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca ataaacttga
    30061 tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg aagtggtccg
    30121 catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc agcatgagag
    30181 cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta acaactgcat
    30241 ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact tgaattacat
    30301 gctgttgcag aaaatcacgt cactcctctt tatcagttaa tctgtaaaaa taaaacctgg
    30361 ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac gcgaaaaacg
    30421 gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat gattttcaaa
    30481 gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa taaaaccgct
    30541 gaaattggct actggctgga cgaatctcat caggggcagg ggatcatttc tcaggcgctg
    30601 caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt gatcaaatgt
    30661 cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat ccttgaaggt
    30721 tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt atacgcgcgt
    30781 attatcgatt cacaataacc ctgccagcgg cgttcgcgta atgcgctcct cgccgttaat
    30841 gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga cggcgtggtc
    30901 ggttaactcc acatggtttt caattatcac cgcacctgta atacggctct taccttgtat
    30961 tacaacgtgc tcgtcgagca gaatcggtcc gccacgtacc acggcatttc cgccaatcaa
    31021 gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca cctgcgaact
    31081 gtaatgaatc gtcggaatgg catcttcttc aatgccagct ttcacctgcg cgtgaccgta
    31141 gactttagcg caatcgcata gccagacgtt gttctcttca ttaccttcaa tactggcaaa
    31201 atcaaaaact tcggcgcgat gttcaataaa agcataccgg atgacggcat cgccataaat
    31261 ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa tttgtagcag
    31321 gagttgatgg tcaggcgtta agccttgtgc tgcgacgatc atagaatgtt gattaattaa
    31381 ggcgtgacca aatattctac attgcccgca aaccagtgag tcatgaatgg tgacactgtc
    31441 acttatatat gcaccctggc tgatttcact attatcgatc cagacattat ccgttgcgta
    31501 aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccggaaa tcaccgttcc
    31561 gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg ctaacaccgt
    31621 ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat cgctaatggc
    31681 gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac taaaggctcg
    31741 ctgttcttca cttagacgat atttgcgcat agcgttttcc cacaggtgac ttactataac
    31801 cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg catttcaaat
    31861 atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt gctcaatttg
    31921 ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc ctggcgctat
    31981 gccagccagg tttggcaggt cagccactgg ttaggggatg ggttggtgat tctggcgatg
    32041 atcatctggg gattattgac tagcgcattt attgcccgac tcatacgctt tccgcatagc
    32101 gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt tccggcaacg
    32161 acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt gtgcctgttc
    32221 agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg attatggcgc
    32281 ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt tgccaacaac
    32341 tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct ggtgttttta
    32401 ggcgcaggcg ttttctcatg gctaagcctt gaaccggtga tcttgcagcg tctgcgtagt
    32461 tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc tcctgcgctg
    32521 gtggcttgta gtgcctggct gagcgtcaac ggcggcgagg gtgacacgct ggcgaaaatg
    32581 cttttcggtt atggactgct gcaactgctg tttatgctac gtctgatgcc atggtatctc
    32641 tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc actggcaacc
    32701 accggtttgc atctggggag tggcagcgat aatggatttt tccatacgct ggcggtgccg
    32761 ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt tgcgcttctg
    32821 atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc agaggacaaa
    32881 gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc cgcacacact
    32941 ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat ctgggctgtg
    33001 gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac gcatgggata
    33061 aaaatgccat gagtatcgcc aacgtcgagc gcattaaatc tattgaaaat ctggataatt
    33121 tacacacccg ggtcgttgat ctgaataacc tcacatttgc tggacagtac gattttattc
    33181 tttcgactgt ggtgctgatg ttccttgagg ctaaaaccat cccagggctg attgccaata
    33241 tgcaacgttg cactaaacct ggcggttaca acctgattgt ggcggcgatg gataccgctg
    33301 attatccatg taccgtcggc ttcccgtttg ccttcaaaga aggagaatta cgtcgatatt
    33361 acgaaggctg ggagatggtg aaatacaatg aagacgtcgg cgagctgcac cgcaccgacg
    33421 ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa aaatgacccg
    33481 gtaagaacaa agcgcgtgaa aattccccac gctgagatgg tttactgtcc ttcttttcgg
    33541 taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag ttttttagca
    33601 gcttaactta ctgaaattta actactgatg attgacttag ccccttttgc ggcattgact
    33661 atgtcgtctg aaaaggggct aaaaaaattt attttcacca acactttttt tgccacaaca
    33721 cgaagcggcg ctttttgcta taacttaaaa agtaatataa tcatctcagg aaactattca
    33781 tgcgtaccac atcatttgcg aaagttgcag ctttatgcgg cttgttggct ctgtctggtt
    33841 gtgcctctaa aatcacccag ccagataaat attctggttt tttaaacaat tactctgatt
    33901 taaaagaaac aacctcagca acaagtaaac ctgttttacg ttgggtagac ccgagttttg
    33961 atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg gtaccaaaac
    34021 cttctaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa
    34081 tgaaagaagc gatagcgcag cgtaaaccag tggttaccac cgctgggccg cgtagtctga
    34141 ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag
    34201 tggttcctgt tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg
    34261 atactcgcct ctattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca
    34321 aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg
    34381 aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttgaca
    34441 aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tatccggtgc gccgtaaaac
    34501 cccgtccttc agggcgggga tatacggcgc ggttttccac ctaaccaggt gtttgctgtt
    34561 gttcaatgta ttgacggatg gcggatattg gcgcacctcc gcagctactg gcaaaatagc
    34621 caggactcca caaaacgcct ttgtagtaat acctgactgc aatatctggt cgatctcgtc
    34681 gcagtaacct acccgatacg cctttgaggc tgtttaccag actggatatc gccagtttgg
    34741 gaggatagtt aattaacaaa cgtgatctgg ttcgccatcc atttcaacca gttcagcttc
    34801 aaaatcagca catacctttg aaaagtaagt gcgtagtttt tctgtcgcgt catggtcaaa
    34861 aatctggcgt cggtatctgg tgacaaagac caggtgaaca tgcatcagga aaacacaatg
    34921 ccttccacgc cggatatcgg tttctttttt cacagaccaa agtatcattt ggcctgtgaa
    34981 acgactacag gcatttaaat tccagttaag acccggtggt caacaggagc gtgaaatgag
    35041 gcgcttcgca ggcgcatgtc gtttcgtttt caatcgtgct ctggcgcttc agaatgagaa
    35101 tcatgaggcc ggtaataaat acatccctta cgggaaaatg gcttcctggc tggttgagtg
    35161 gaaaaatgcc actgaaacgc aatggcttaa agatgccccg tcacagccat tgcaacagtc
    35221 actgaaagac cttgagcggg cttacaaaaa cttcttccgg aagcgggctg cttttccccg
    35281 attcaaaaag cggggacaga atgatgcatt ccgctacccg cagggcgtta agctcgatca
    35341 ggaaaacagt cgtatttttc tgccgaaact tggctggatg cgctaccgga atagccgtca
    35401 ggtcacgggt gttgtgaaaa atgtcactgt cagccagtcc tgcggtaagt ggtacatcag
    35461 tattcagact gaaaatgaag tatcaactcc ggttcaccct tcagcattaa tggtcggact
    35521 ggatgctggc gtggctaaac tcgccacgct gtcagatggc acagtctttg ggcctgtaaa
    35581 cagttttcag aaaaaccaga agacgctggc gagacttcag cgacagttaa gccgcaaggt
    35641 caaattcagc aacaactggc agaagcagaa acgcaaaata cagcgactgc attcctgtat
    35701 cgcaaatatc cgcagggact accttcacaa agtcacaacg accgtcagca aaaaccacgc
    35761 aatgatagtc attgaggatt tgaaggtcag caacatgtca aagtcagcag cgggtacggt
    35821 cagccagccg gggcgcaatg tccgggcaaa atcaggttta aaccgttcga tactggatca
    35881 gggctggtat gaaatgcgcc gccagcttga gtacaagcag ctctggcgtg gcggtcaggt
    35941 gcttgctgtt ccgccagcgt acacaagcca gcgttgcgcg tgctgtggtc atacagcgaa
    36001 agaaaatcgc ctgtcacaaa gtaaattcag atgccaggca tgtggatata cagcgaacgc
    36061 tgatgtaaat ggcgctcgta acattttagc ggcggggcac gccgttcttg cctgtggaga
    36121 gatggtgcag tcaggccgcc cgttgaagca ggaacccacc gaaatgattc aggcgacagc
    36181 ctgaacgtag cagggatcca cgtcct
//
LOCUS       DAZGCQ010000047        35342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000047 DAZGCQ010000000
VERSION     DAZGCQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.047"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..800)
                     /locus_tag="ACHK07_002485"
     CDS             complement(<1..800)
                     /locus_tag="ACHK07_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO8102802.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGVPGLNREN
                     FIYISNIFLNIKQRNEKNHSINMFREVSISGDIVSVKFYRNEKIECACDFMMAKDAQG
                     YIDLSELDLTSCHFKGDVISKVSFISSNLQHVTFECKEIGDCNFTTAIVDNVIFKCRR
                     LHNVIFIKASGDYVDFSKNILDTVDFSQSQLTHSNFCECQIRNSNFDHCYLYASHFTR
                     AEFLTDKEISFIKSNLTAVMFDHVRISTGNFKDSVTQLMVLSIDYSDV"
     gene            complement(1029..1949)
                     /gene="malM"
                     /locus_tag="ACHK07_002486"
     CDS             complement(1029..1949)
                     /gene="malM"
                     /locus_tag="ACHK07_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO8102803.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVAGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(2192..3532)
                     /gene="lamB"
                     /locus_tag="ACHK07_002487"
     CDS             complement(2192..3532)
                     /gene="lamB"
                     /locus_tag="ACHK07_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO8102804.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3604..4719)
                     /gene="malK"
                     /locus_tag="ACHK07_002488"
     CDS             complement(3604..4719)
                     /gene="malK"
                     /locus_tag="ACHK07_002488"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO8102805.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5084..6274
                     /gene="malE"
                     /locus_tag="ACHK07_002489"
     CDS             5084..6274
                     /gene="malE"
                     /locus_tag="ACHK07_002489"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO8102806.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6428..7972
                     /gene="malF"
                     /locus_tag="ACHK07_002490"
     CDS             6428..7972
                     /gene="malF"
                     /locus_tag="ACHK07_002490"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO8102807.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7987..8877
                     /gene="malG"
                     /locus_tag="ACHK07_002491"
     CDS             7987..8877
                     /gene="malG"
                     /locus_tag="ACHK07_002491"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO8102808.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            9249..10724
                     /gene="xylE"
                     /locus_tag="ACHK07_002492"
     CDS             9249..10724
                     /gene="xylE"
                     /locus_tag="ACHK07_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO8102809.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10768..11178)
                     /gene="psiE"
                     /locus_tag="ACHK07_002493"
     CDS             complement(10768..11178)
                     /gene="psiE"
                     /locus_tag="ACHK07_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO8102810.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11304..11447)
                     /locus_tag="ACHK07_002494"
     CDS             complement(11304..11447)
                     /locus_tag="ACHK07_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102811.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11448..11726
                     /locus_tag="ACHK07_002495"
     CDS             11448..11726
                     /locus_tag="ACHK07_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102812.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11773..13869)
                     /locus_tag="ACHK07_002496"
     CDS             complement(11773..13869)
                     /locus_tag="ACHK07_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8102813.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13869..14606)
                     /locus_tag="ACHK07_002497"
     CDS             complement(13869..14606)
                     /locus_tag="ACHK07_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8102814.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            complement(14603..15241)
                     /locus_tag="ACHK07_002498"
     CDS             complement(14603..15241)
                     /locus_tag="ACHK07_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO8102815.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15355..15597)
                     /gene="yjbE"
                     /locus_tag="ACHK07_002499"
     CDS             complement(15355..15597)
                     /gene="yjbE"
                     /locus_tag="ACHK07_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO8102816.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(16109..17758)
                     /gene="pgi"
                     /locus_tag="ACHK07_002500"
     CDS             complement(16109..17758)
                     /gene="pgi"
                     /locus_tag="ACHK07_002500"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO8102817.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17995..18191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            18283..19632
                     /gene="lysC"
                     /locus_tag="ACHK07_002501"
     CDS             18283..19632
                     /gene="lysC"
                     /locus_tag="ACHK07_002501"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO8102818.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLSMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            19885..20157
                     /locus_tag="ACHK07_002502"
     CDS             19885..20157
                     /locus_tag="ACHK07_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO8102819.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(20158..21030)
                     /gene="rluF"
                     /locus_tag="ACHK07_002503"
     CDS             complement(20158..21030)
                     /gene="rluF"
                     /locus_tag="ACHK07_002503"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO8102820.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21378..22325
                     /locus_tag="ACHK07_002504"
     CDS             21378..22325
                     /locus_tag="ACHK07_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO8102821.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            22395..23201
                     /locus_tag="ACHK07_002505"
     CDS             22395..23201
                     /locus_tag="ACHK07_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8102822.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            23211..23618
                     /locus_tag="ACHK07_002506"
     CDS             23211..23618
                     /locus_tag="ACHK07_002506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO8102823.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            23618..24112
                     /locus_tag="ACHK07_002507"
     CDS             23618..24112
                     /locus_tag="ACHK07_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO8102824.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            24178..24975
                     /locus_tag="ACHK07_002508"
     CDS             24178..24975
                     /locus_tag="ACHK07_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO8102825.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24986..25810
                     /locus_tag="ACHK07_002509"
     CDS             24986..25810
                     /locus_tag="ACHK07_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO8102826.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25862..27091
                     /gene="sorE"
                     /locus_tag="ACHK07_002510"
     CDS             25862..27091
                     /gene="sorE"
                     /locus_tag="ACHK07_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO8102827.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSMVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSESQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            27473..28162
                     /gene="pepE"
                     /locus_tag="ACHK07_002511"
     CDS             27473..28162
                     /gene="pepE"
                     /locus_tag="ACHK07_002511"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO8102828.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(28253..29884)
                     /locus_tag="ACHK07_002512"
     CDS             complement(28253..29884)
                     /locus_tag="ACHK07_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO8102829.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(30104..33787)
                     /gene="metH"
                     /locus_tag="ACHK07_002513"
     CDS             complement(30104..33787)
                     /gene="metH"
                     /locus_tag="ACHK07_002513"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO8102830.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRVRKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLETARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33987..34811
                     /gene="iclR"
                     /locus_tag="ACHK07_002514"
     CDS             33987..34811
                     /gene="iclR"
                     /locus_tag="ACHK07_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO8102831.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            35128..>35342
                     /locus_tag="ACHK07_002515"
     CDS             35128..>35342
                     /locus_tag="ACHK07_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102832.1"
                     /translation="MITRIPRSSFSVNINNTAQTNEHQNLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIK"
BASE COUNT         8448 a         9147 c         9042 g         8705 t
ORIGIN      
        1 aatacatctg agtaatcaat agataatacc attagttgtg taacgctgtc tttaaaattc
       61 cctgtcgata ttcgcacatg atcaaacata acagctgtta aattcgattt aataaatgat
      121 atttctttgt cagtaagaaa ttctgctctg gtgaagtgcg aagcataaag ataacaatga
      181 tcgaagtttg aatttctaat ctgacattca caaaaattac tatgagtaag ttgactctgc
      241 gagaagtcaa ctgtatcaag aatatttttg ctaaaatcga cataatcacc actcgctttg
      301 ataaaaatta cattgtgtaa acgtcgacat ttaaatatga cattatcaac tattgcagta
      361 gtaaaattgc aatccccaat ttctttacat tcgaatgtta catgttgtag atttgatgat
      421 atgaaagaca ccttcgaaat aacgtcacct ttaaaatgac aacttgttaa atccaattca
      481 gacaggtcga tatacccctg tgcatcttta gccatcataa aatcacaagc gcattctatt
      541 ttttcatttc tataaaattt tacgcttaca atatcaccac tgattgagac ttcacgaaac
      601 atatttattg aatgattttt ttcgttccgt tgttttatat ttaaaaaaat attgctgata
      661 taaataaagt tttctcgatt taaccctggc accccattaa aattacgtag taaatcaaaa
      721 tctactatat tatttccttg caccacacca tgttttatca aagagttatt gccgtcaaac
      781 tttgagatat gacctaacat aaaacctctg cattacagaa atgaaataac ctggaaaagt
      841 agcctgaatg tccagacact accggcgaaa ggaaaagaat ctctgataag gcattgagat
      901 aatggatatt cttagtgaag aagaagtgag ttactataaa aaatcgctca tatcccagga
      961 atgaagcata aaaaaaggtg cgccaggaga cgcaccagtt gttgcaaaat cagcactgtg
     1021 gggcgtaatt accccttgcc ttttacactg ctgataaagg tggaacgggc agatgtcgat
     1081 cccaggcgtt cagcttcatc aagcagtttt aacgccttat caacatcacc tttcgcgaca
     1141 gcgtttttga tcgcggtatt aaaataactt tccgtgtcgt tgagcatcgg ctcgcttttc
     1201 ttcaccggca ccggagcggg tgcagccaca gctggtgccg ccgtgttgcc taccgtaacc
     1261 ggagctggag cggaagaacc aaataaaggc ccgaccaaca cgctggagct ggagttcgtt
     1321 ttcactttca gtttcagtaa gccatcggtg gtatgacgag caaccggatc gggaatatcc
     1381 gggatcgagt taccaacgcc tttggcatag gctttagccg ggtcgagcag ttgggtcgtc
     1441 tgctggagat ctttttccgt ggtaaagacc agaacataaa gtttttgctg ccccaacgcc
     1501 ggtgtcaggc gcataacgcc ttccagccga tctgcactca tcacgcctgg ttcctggtag
     1561 gtgaaataac tgctgggaaa gaaggctgat ggggtcatgt tctgatcaag aatcagcacg
     1621 ttcggcgcaa atacgctggt ttgtttgttc acttcgctgg tcagcgtcag ggtcagttcg
     1681 ccaatgtttg ccgggacgct gtacgcagca accggaccac tgatgccagc aacgttcagt
     1741 tgttggccgc cagtcgccag ttgggtggtc tgggttttag attgatcgac cggtgtccag
     1801 gtgagttgtt gcagcgcggc agatggaatg gctggcgcgt cgctggtgtt ttgcggtacg
     1861 tagttaacat cggcaaggct gattccaggc gcgcttgcca gcaaccctgc tgataaacag
     1921 agggcgatga gacttttatt cattttcatt gttttcacct caaaatctgg agctcagcgg
     1981 tagccaggca atagcgcgct aaacccgata atcagagggg cttgcgcccc tttcagataa
     2041 tgacaacctg tttttatgcc ggatgcggcg taaacgcctt atccggccta catttgacag
     2101 ccgttgtagg cctgataaga cgcgcaagcg tcgcatcagg cgttggatgc cggatgcggc
     2161 gcaagcacct tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga
     2221 aggtccactc gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg
     2281 ctttgccgta gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat
     2341 cccacttggc gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag
     2401 cctgccattg ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg
     2461 attcgacgtt gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca
     2521 tcgggcgaat accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct
     2581 ggtacatacc cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga
     2641 ggatacgcag catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaatg
     2701 caacgccaga accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact
     2761 gaacaacaaa cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc
     2821 agccgtcttt cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt
     2881 agtcgacacc cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat
     2941 cgaaaacgtc gttcgcggtt tcgttggtat agtcataaat attgttgctg gcgaaagagg
     3001 aagaaccacc tgcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat
     3061 cgatgttttc cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat
     3121 gaacgtcatg acgttggtag aagcgcttac ctgcccagat ggtggagcct ggcagccatt
     3181 cgatcaggtt tttaccctgc acgtttgctt cacggaaggc cgggtcggta gcttcccagt
     3241 cattctgttg cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct
     3301 ctttccacac ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac
     3361 ggtatttact ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc
     3421 agccaatacc ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta
     3481 cgcccgctgc gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc
     3541 ctgagtcatt gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg
     3601 tgcttaaacg cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag
     3661 atggcaacgc tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac
     3721 gtcgttctgg cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt
     3781 ttcgttgccg agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc
     3841 actcggcagt agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc
     3901 acggctttca actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac
     3961 ttgatcgatt gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat
     4021 aaatccggcg acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg
     4081 cgccacgcga ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg
     4141 atcgtgggtg acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat
     4201 acgcatttgc acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac
     4261 gcttggctcg gccaccagcg tacggccaat cgccacgcgc tgacgctgac caccggagag
     4321 cgctttcggt ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac
     4381 gcgttggtta atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc
     4441 tgctactgac aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc
     4501 tgctggcgga gtgtcattca tccgtttctc accgatgaac aagtcgccgc tggtgatcgt
     4561 ctcaagcccg gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa
     4621 caccacaaat tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcacc
     4681 ccaggctttc gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa
     4741 cctgtcatcg acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa
     4801 atcctccacc ccctggcttt tttatggggg aggaggcggg aggatgagaa cgcggcttct
     4861 gtgaactaaa ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga
     4921 ttttatgtgc gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg
     4981 gtggggcgta ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt
     5041 ttaggtgttt tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg
     5101 tgcacgcatc ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc
     5161 caaaatcgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct
     5221 cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc
     5281 ggataaactg gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat
     5341 cttctgggca cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac
     5401 cccggacaaa gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa
     5461 cggcaagctg attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga
     5521 tctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa
     5581 agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct
     5641 gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga
     5701 cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa
     5761 aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg
     5821 cgaaacagcg atgactatca acggcccgtg ggcatggtcc aacatcgaca ccagtaaagt
     5881 gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaagc cgttcgttgg
     5941 cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct
     6001 cgaaaactat ctgctgactg acgaaggtct ggaagcggtt aataaagaca aaccgctggg
     6061 tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgcagccac
     6121 catggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg
     6181 gtatgcagtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc
     6241 cctgaaagac gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg
     6301 aacgccttgt ccggcctaca aaaccgaaac gtatgtaggc ctgataagac gcgtcagcgt
     6361 cgcatcaggc agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa
     6421 gaaccccatg gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc
     6481 agtgttaggt ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg
     6541 ggaatacctg ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc
     6601 caatcgtaaa gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt
     6661 cgtcctcttc cctctggtct gcaccatcgc cattgccttc acaaactaca gcagcactaa
     6721 ccagctgact tttgaacgtg cacaggaagt gttgttagat cgctcctggc aagcaggcaa
     6781 aacctataac tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagcgacgg
     6841 cgaaaccggc aaaaattacc tctccgacgc ttttaaattt ggcggagagc aaaaactgca
     6901 actgaaagaa acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca
     6961 gaatcgtcag gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat
     7021 gagctccctg cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac
     7081 gttgacgaat aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca
     7141 gtccattacc gccgacggca actggggcga tgaaaagcta agccccggtt acaccgtgac
     7201 tactggctgg aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct
     7261 cgccattttc gtctggaccg tggtgttctc gctgatcact gtctttttaa cagtggcagt
     7321 cggcatggtt ctggcgtgtc tggtgcagtg ggaagcgttg cgcggcaaag cggtctatcg
     7381 cgtcctgctg attctgccct acgcggtgcc atcgttcatt tcaatcttga ttttcaaagg
     7441 gttgtttaac cagagcttcg gtgagatcaa catgatgttg agcgcgctgt ttggcgtgaa
     7501 gcccgcctgg ttcagcgatc cgaccaccgc ccgaacgatg ctgattatcg tcaatacctg
     7561 gctgggttat ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga
     7621 tttgtatgaa gcctcagcaa tggatggtgc aggtccgttc cagaacttct ttaagattac
     7681 gctgccgctg ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt
     7741 taacaacttc gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac
     7801 cacgccagcc ggttataccg acctgcttgt taactacacc taccgcatcg cttttgaagg
     7861 cggcgggggt caggacttcg gtctggcagc agcaattgcc acgctgatct tcctgctggt
     7921 aggtgcgctg gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata
     7981 acaaaaatgg caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg
     8041 ctactgctac tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgccatctca
     8101 ctgcgccagg ggaactttgc gaccggtagc ctgatcccgg agcaaatctc ctgggatcac
     8161 tggaaactgg cgttaggttt tagcgttgaa caggctgatg gtcgcattac gccaccgcca
     8221 ttcccggtac tgctgtggct gtggaactcg gtaaaggtcg ccgggatttc cgcgattggc
     8281 attgtggcgc tctccaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa
     8341 gcgacgctgc tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc
     8401 gcgttgtatg cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact
     8461 cacggcggcg taattttcgc gtatctgggt gggattgcgc tgcacgtctg gaccatcaaa
     8521 ggctatttcg aaaccatcga cagttcgctg gaagaagcgg cggcgctgga tggtgcgaca
     8581 ccgtggcagg ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggtg
     8641 ttcatcctgt cgtttatcgc tgccattact gaagttccgg tcgcatcgct gttactgcgt
     8701 gacgtaaaca gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac
     8761 ctgtggggtg actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc
     8821 ttgctagctc aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga
     8881 tgttgttctg ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact
     8941 gttattcggc gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc
     9001 ggttaatatt tccatcatag aacttattat ttttacgtta tttgttttcc cacttacgat
     9061 aattctcttt cgtgctctga gtcacggcaa tagtattgtt tttatcaatt ttggataatt
     9121 atcacaatta agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc
     9181 cttaactgcc tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc
     9241 aggtctgaat gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta
     9301 cattaggtgg tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac
     9361 tcaataccgt ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag
     9421 ggttttgcgt agccagcgct ctgattgggt gcatcatcgg tggtgccctc ggtggttatt
     9481 gcagtagccg cttcggtcgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt
     9541 ctggtgtagg ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag
     9601 tgcctgttta tctggcaggt tatgtcccgg aatttgttat ttatcgcatt attggcggta
     9661 ttggcgttgg tttagcctca atgctctcgc ccatgtatat tgcggaactg gctccagctc
     9721 atattcgcgg gaaactggtc tcttttaacc agtttgcgat tattttcggg caacttttag
     9781 tttactgcgt aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg
     9841 gctggcgtta tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt
     9901 ataccgtgcc agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaag
     9961 gtatcctgcg caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac
    10021 actccctgga tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga
    10081 ttgtcatcgg cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt
    10141 actacgcgcc ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga
    10201 ccattattgt cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata
    10261 aatttggtcg taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta
    10321 gcctcggtac cgcgttttac actcaggcac cgggtattgt ggcgcttctg tcgatgctgt
    10381 tctatgttgc cgcctttgcc atgtcctggg gtccggtatg ctgggtactg ctgtcggaaa
    10441 tcttcccgaa tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg
    10501 cgaactactt cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc
    10561 atttccacaa cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat
    10621 ttatgtggaa atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct
    10681 gggaaccgga aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac
    10741 gccgcgccag ttcggcgtgc tgtcttttta ctcccgcttc agccgtttcg aattacacag
    10801 ccacagggtg atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga
    10861 tttatgatcg acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg
    10921 tagcgggaag tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata
    10981 gagaaagtaa accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa
    11041 tagcacatca gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag
    11101 gcacaacagg ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg
    11161 acgagagagt gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca
    11221 aaaatgtgac ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc
    11281 gcctccacca aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca
    11341 tccggcaaga caaaccgcac caaagccggt aattcaaaaa tcacccattc aggtgattcc
    11401 cagccaccag caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc
    11461 tgataaaggt ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc
    11521 ttcctgctta tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg
    11581 tctccagccc aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg
    11641 acaggaagac agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg
    11701 ttcctgatgg ctcgacaggc cattaagaat gaaggtgcca gtgagtgcga aaactggcac
    11761 ctttcccatg acttagcgga aattgacgct acggtcgctg gtcatgtcat acaactggaa
    11821 cttacgccca agttgctgac caccgtcacg cgtcagcggc gtccagccaa ttgccgcacg
    11881 gctgcgtgtc gggccagacg agaagagatc caacggtaca gagacataca cgcctttggt
    11941 gaagtcccct tcgccgtact cctctttcga aacattagtg atcgtggcat agccacccac
    12001 caccacaccg ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag
    12061 atactgcccg acgctggctt taactaacac atcctgagcg aaagatggcg tccagtaggc
    12121 ggtcagatgt ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg
    12181 ccagtcgcgc tgtttaacgt agttggcatc cacaccaaac gcccagttgc tgtccagcgg
    12241 gcgatacaac acttctgccc ccgcaccgcc aaacatggtt tcgagatagc cgccgtagac
    12301 ctgaccgtag aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata
    12361 gacatcgttc tgcacatact cgcgcacatg ggtacgcacg cgcggtaagt gcgagtcctg
    12421 cggcggattg gtgtagttaa acttgtcgta gttgttggca aggttagcga acaggctgcc
    12481 ggtggtcagc agatggtccg tcagccacaa atctgccgtt cccatcacgc cgagctgata
    12541 catgtaaaag ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc
    12601 aaagcgtgat ttgtcgatat accagccctg ctcggtggac ttcggaacca ctggctcgac
    12661 gcgtttttgc gccagcttcg tttcgtggcc caacggctct ccggcgagat gatttttcag
    12721 gctggcgaca tccgtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaacgcg
    12781 gatcgtcttg atcccatccg gcagatcatt catcacgatc cgattagcac ggatgatccc
    12841 ttcgcgcgaa tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc
    12901 ctggatctgc ggatcggcaa gtccggcatt gtatttcaac agcgttaact gattcgccac
    12961 caccgaatgc tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc
    13021 gttgtaagac gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt
    13081 gttgccacgt tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc
    13141 gacgttaaac ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattgcc
    13201 ttcatactcc agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa
    13261 cagtgaggca ggaccgtgga acatctggct accgtcgata gatcccgcct gtttgtagct
    13321 gttatcgcga tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct
    13381 ggtgcccaga tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct
    13441 ggcaacaaga tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc
    13501 cacttgcggc agccaataac tctcttccca caaacgcagt ttgagatcga aggctttatc
    13561 tttatacgtt tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc
    13621 ggtgtagcgc agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg
    13681 gtactgatcg ttatcacgat agttcagact caactcccct tcccgtgcca tacgcgcggt
    13741 gggcgtttgt aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg
    13801 atatgtttcg ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct
    13861 ttttttcatt attgaggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca
    13921 tcaggcatct cactccagac ggagtccgcg aggccaacat aaataatgct gccgggcatc
    13981 ggctctacgt gacgcttgtt ccagtaagcc accggcgctt tttgtgtgcg tccgtccggg
    14041 taaaccaccc acgcgtagct acgatccgca ccgctaagca gactttgccc ggagagatag
    14101 ctcgccacgt cgcgaccggg agtgaatggc tgattgccag gacggctgat aagcccgaac
    14161 aacgtgaccg tggacggtgg tggtccgacc cacagcgtgt agttgccctg caacggcggg
    14221 ttagcgcgtt cagcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcccgtc
    14281 accttcaacg cctgaatttg ctggcgtaag gcgttaatcg cagcggcatc gtcggcgctg
    14341 gagtctgcgg ccagttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc
    14401 gccgttgcca gctcttcgct gatcaccgca ccgggccacc agctgtttgc cagccgcggt
    14461 tggcctacca gatcgattaa atgctctgcg cctgttagtg ttttggcctc actgctgcca
    14521 ttgctgaaca ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc
    14581 aacgcgacaa aagtttgttt gatcattgtt ttgccgcctt gatgagggtg gttttcaccg
    14641 ggaaataatc cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc
    14701 aatagcggtt atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaaccg
    14761 gggtttcatc gctgccgact ttgacggtat cggtgccatc ccatttgaag actgagcgtg
    14821 cggtggcgta gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac
    14881 catcaacaat ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca
    14941 ggttgtcgcc gccaagcaaa gtcttcacca gacgaccatg ttgtgtgacg agagtggcct
    15001 gatcctgagt gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaact
    15061 gcggcccgcc attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt
    15121 ccgtcagctg tacacctggc gtaccgaaca gactgtccca cagtgaattg cccagctctt
    15181 tagtcgtggc tgaacaggcc tgtaatagca ggcagataag aatgaatgca ggtcgcttca
    15241 cgactcttct ccagaggggc agaataacca caccggagtg tggttatggt aaatcccttc
    15301 atacttcaag ccacagatgc gatgactcga attattttgg ggatacatac tttattactg
    15361 ggtactggtg gtcgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc
    15421 cgcgagcgcg acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactacc
    15481 cgccgaaacc gacgttgctg ccgaccctgc cgcttcgccc acctgtacag gtgcagccca
    15541 cgcagaagtc gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac
    15601 aatttccctt cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta
    15661 tacacaacta aagcatgcga attacggcgc ggggaattag agatggggtt ttaggataat
    15721 tggaatgaat atcaaatata agctacaact tatatataaa taacattaaa aatcatagtg
    15781 ttaaaataaa agtaaaagta attaatctga gtattttccg aaatcaatca cgattaaaaa
    15841 tctattttag gattaacctc acggtatgat ttccattaaa tcacagacaa ggagatttat
    15901 ctgataaaac aatgccgact ttaagtcggc attgttatta aggttattta attaatcgtg
    15961 ctatgtatta attgccgaat acgatgtaca tatcggcatc gacctgtagg cctgataaga
    16021 cgcgaccgcg tcgcatcagg catcggttgc cggatgcggc gtgaacgcct tatccggcct
    16081 acatagcggc atcaggcatg aacgatgatt aaccgcgcca cgctttatag cggttaatca
    16141 gaccattggt cgagctatcg tggctgctga tttctttatc atctttcagc tctggcagaa
    16201 tacggttcgc cagctgttta cccagttcca cgccccactg gtcgaaggtg aagatgttca
    16261 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    16321 acggagtgat ttcacgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16381 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16441 ccacttcgcg ggatttacca aacgccagcg cttcggtctg agcaaagaag ttagacagca
    16501 gtttctggtg atgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16561 gtaccatttt ggttccctga tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16621 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16681 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcaaaacgg tgcatatact
    16741 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16801 tcagcgccag caagacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16861 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16921 acaggccaat cgctgaccac aaagagtaac gaccgccgac ccagtcccag aactcgaaca
    16981 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggag agcgccgcaa
    17041 agtgtttcgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    17101 gggcgttggt catggtttcc tgagtggtga aggttttaga tgctaccagg aacagggtag
    17161 tttccgggtt tacttttttc agcacttccg cgatgtgggt gccatcgacg ttagacacaa
    17221 agtgcatgtt caggtggttt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    17281 ggtcagaacc gccgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    17341 tccactcacc ggagataatc gcttgtgaga aggttttcat cttctccagc accgcgttga
    17401 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17461 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17521 taatcgcgcc cgccagatcg cactctttcg ccaaatcttg taatttcgcc agcgtctctt
    17581 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaaggtt gcggagaact
    17641 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17701 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17761 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17821 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17881 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17941 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    18001 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    18061 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    18121 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    18181 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    18241 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    18301 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18361 gtgctttctg atgccaacgt acgtttagtt gtcctctcgg cttctgctgg tatcactaat
    18421 ctgctggtcg ctttagctga aggactggaa cctggcgagc gattcgaaaa actcgacgct
    18481 atccgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18541 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18601 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18661 gttgagatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18721 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18781 gcgctgcagc tgctcccacg tctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18841 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18901 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18961 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    19021 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    19081 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    19141 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    19201 aatcagactc tgctcacttt gcacagcctg agtatgctgc attctcgcgg tttcctcgcg
    19261 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    19321 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19381 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaggtgga agaaggtctg
    19441 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19501 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt atggcgcatc cagccataac
    19561 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcagaagct gcattttaat
    19621 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19681 tcactatgct catcaataaa cgagcctgta ctctgttaac cagcgtcttt atcggagaat
    19741 aattgccttt aattttttta tctgcatctc taattaatta tcgaaagaga taaatagtta
    19801 agagaaggca aaatgaatat tatcagttct gctcgcaaac gaattcccgt acactgaact
    19861 ctctggctca ctgagggcac catcatggca ctcccccgta ttacccaaaa agagatgact
    19921 gaacgcgagc agcgtgaact taaaacactg ctggatcggg cgcggattgc gcatggtcgc
    19981 gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc
    20041 gagcgtgagg ctgaagcgaa aaaagcccgc cagttaaaga aaaagcaggc ttataaaccg
    20101 gatcctgaag cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctgatta
    20161 gcgccccttc tttttacgcc ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc
    20221 gcctttttcc gccgtttttt ccatcttaac gactggacgt ttgatgcccg ccgttttcgg
    20281 tttggccttc gttttaggtt ttgcctcgga agaggaattt tcgatgagct taaagagatc
    20341 gataagctcg tcgtcggtta aatcgcgcca ttctcccagc ggaatgccgc ttaagctgac
    20401 gttcataatg cgcgtacgtt ccagcttttt cacttcatag ccgaaatgct cgcacatgcg
    20461 acggatctga cggttcagcc cctgcaccag ggtaatgcgg aagacaaacg gcgcttcttt
    20521 tttgacttta cactttttag tcactgtccc gaggattggc acccccgccc ccataccacg
    20581 aataaattca tcggtaatcg gtttatcgac cgtcaccaga tactctttct catgatcgtt
    20641 acccgcacgg aggatcttat tcaccagatc gccgtgattg gtgaggaaaa tcagcccctg
    20701 ggagtcttta tccaggcggc caatcgggaa cacgcgtttg ctgtggttaa cgaaatcgac
    20761 aatgttatcg cgctcgccat cttcggtggt gctgacaatg cctacgggct tgttcagggc
    20821 gataagtacc aaatcttcgg cttcccgagg ctcaatcaac tgaccgttta ctttcacaac
    20881 gtcgccgggt ttcacctgat cgccaatggt ggctcgcttg ccattaagga acacattgcc
    20941 ttgctcgata tagcgatccg cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt
    21001 atttaaacgg actgatgagt cgggcagcat aaattctcct gtaaaacgcg gaatataccg
    21061 tacctcttag cggggaaaaa cagggttatg cggcaacgtc gaaattaaat gtgatctgcc
    21121 acgcctttta tcacaaaagt gtctccggaa gtacaatgca aatttgcgtg gcaaaataag
    21181 gataatcaac ctattacgaa tctccgcaca cctgtgctga taactactac gcagagaaac
    21241 aaatgtgcaa gagaaagcgc tatttctccc catgcaaaga gtgaaaaatc gctttgaaag
    21301 gccatcatga tggggttata tcagatcaag cggtgagaaa gcgtatttgc acaaatgtgc
    21361 agaaccagga ggcggcaatg gaaaacagtg acgatatccg tttgattgtg aagattgccc
    21421 aactctatta cgaacaggat atgacgcagg cgcaaatcgc gcgcgaactg ggtatttacc
    21481 gcaccaccat cagccgcttg cttaaacgag gccgcgatca gggaattgtc accatcgcca
    21541 tcaactatga ctacaacgaa aatctctggc tggagcagca actgaagcaa aagtttggcc
    21601 tgaaagacgt tgtggtggtg tcgggaaatg atgaggatga agagactcaa ctggcgatga
    21661 tggggttaca cggcgcgcaa ctgctggatc gcttgctgga gcctggcgat attgtcggtt
    21721 tttcctgggg tcgcgcggtg agcgcactgg ttgaaaactt gccgcaggcg gggcaatcgc
    21781 ggcagttaat ctgcgtacca attattggcg gaccgtccgg taaactcgaa agccgctatc
    21841 acgtaaacac attaacctac agcgcggctg cgaagctgaa aggggaatcg catctcgcgg
    21901 attttccggc tctgctggat aaccccttaa ttcgtaatgg gatcatgcag tctcagcact
    21961 ttaaaaccat ctctgcctac tgggataatc tggatgtcgc cctggtggga attggctcac
    22021 cggccattcg cgacggcgct aactggcatg cgttttatgg tggtgaagag agtgacgacc
    22081 tgaatgcccg ccaggttgct ggcgatattt gctcgcgctt ttttgatatt cacggcgcaa
    22141 tggttgaaac gaatatgagc gaaaaaacac tctctatcga aatgaataaa ttaaagcagg
    22201 cacgatattc cattggcatt gccatgagtg aagaaaaata cagcggaatt attggtgcac
    22261 tgcgtggaaa atatattaat tgtctggtaa cgaatagcag cacagctgaa ctattactga
    22321 aataaaaatc ttcccgatgt ttatcgtaat aaaaattacg gcgggtccct attatctaaa
    22381 atcaggagtc tgttatgcaa acgtggttaa atttgcagga taaaatcatt atcgtcaccg
    22441 gcggcgcatc cggtattggt ctggcgattg tagaggaatt attagcacaa ggcgcaaacg
    22501 tacagatggt agatattcac ggtggcgatg gtcaatatga agttcataaa ggctatcagt
    22561 tttggccgac cgatatttcc agcgccaaag aggtaaatca tacggtagcg gaaattatcc
    22621 agcgttttgg tcgcatcgac ggtctggtca ataacgccgg ggtcaatttc ccgcgtctgc
    22681 tggtcgatga gaaagcgcct gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa
    22741 tggtcaatat caaccagaaa ggcgtttttc tgatgtcgca ggcggtggcg cgacagatgg
    22801 tcaaacaaca tgatggcgtg attgtgaatg tttcctcaga aagtggactg gaaggctcag
    22861 aaggccaaag ctgttacgcc gctaccaaag ccgcgctcaa tagcttcacg cgctcctggt
    22921 cgaaagagct gggtaaacac ggtatccgtg tggtcggtat cgcgcccggg attctggaaa
    22981 aaaccgggct acgcacgccg gaatatgaag aggcgctggc gtggacgcgc aatatcaccg
    23041 tcgagcagct gcgtgaaggc tataccaaaa acgccattcc tattgggcgc gccggaagat
    23101 tagcagaagt cgctgatttt gtttgttatc tgttgtctga acgcgccagc tacatcaccg
    23161 gagtaaccac taacattgca ggcggcaaaa cgcgcggata aggaggcttt atggtcaacg
    23221 ccattttttg tgcccacggc aaattagctt gcgccatgct ggaatcggtt cagatggttt
    23281 acggcaacac caatgttgaa gccgttgaat ttgtgcccgg tgaaaacgcc ggagacatcg
    23341 tagcaaagct gaaaaagtta gtaagcattc acagtcaaga tgagtggctg attgccgtgg
    23401 atttacagtg cggcagcccg tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc
    23461 tgcgggtaat cagcggactt tctctgccgc tggcgctcga actggttgat aaccaggaca
    23521 gcatgaatgt tgatgaactg tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgttg
    23581 tctggaaaca actggcaacg acagaggagg atttctgatg aacattacgc tcgcccgcat
    23641 tgatgaccgc ctgatccacg gtcaggtcac taccgtctgg tcgaaagtgg ctaacgccca
    23701 gcgcattatt atctgcaatg acgaagttta taacgatgaa gttcgccgga cattattgcg
    23761 tcaggctgcg ccaccgggaa tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt
    23821 ttatcacaac ccgcaatatc aggacgaaac cgttttttat cttttcactc gcccacagga
    23881 tgccttagcg atggttcgcc agggggtaaa aataggcacc ctaaatattg gcggcatggc
    23941 ctggcggccc ggtaaaaaac agttaaccaa agcggtttca ttagatgatg atgatattaa
    24001 cgcatttcat gagttaaata atcttggcgt catactggat ttacgcgtcg tagcgtcaga
    24061 tccctcaatc aatattatcg acaaaataaa cgaacagtta atcgcaaatt aaataatagc
    24121 gcctgtgtac atatgcttta acaggcaggg atgacgacac ttaaaaggtg acaaatcatg
    24181 gaaataagta ccctacaaat tattgctata tttctttttt cctgtattgc cggaatgggc
    24241 agcgtgctgg atgaattcca gactcatcgc ccgttaattg cctgtacggt gattggttta
    24301 attctcggtg atttaaaaac cggaataata ctcggtggta cgctggaatt gatagctctc
    24361 ggctggatga acgtcggcgc ggcgcaatct ccggattctg cactcgccag cataatctcc
    24421 gccattctgg ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct
    24481 gtggctgcgg caggccaggt gctgacggtg tttgcacgta ccatcaccgt agtgttccag
    24541 cacgcggcgg ataaagcagc ggaagaagcg cggtttcgca ctctcgatat tctgcatgtc
    24601 tccgcgcttg gcgtgcaggc gctgcgcgtc gccattccgg cactgattgt ctcgctgttc
    24661 gtcagcgccg atatggtcag caatatgtta aacgccattc ccgaatttgt gacccgagga
    24721 ctacagattg ctggcggttt tatcgtggtg gtcggttacg ccatggtgct acgtatgatg
    24781 ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat
    24841 ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg ccctggtcta catccagcta
    24901 aatccacagt ggcgtaaagc tgaacctcaa ctccaggcca ccgcctccac cgcccttgac
    24961 caacttgacg attaacggag ccatcatgga acagagaaaa attacacgca gcgatctggt
    25021 gagcatgttt ctgcgctcca acctgcaaca ggcgtccttt aactttgaac gtattcacgg
    25081 gctgggtttt tgctacgaca tgatccccgc catcaagcga ctttacccat taaaagagga
    25141 tcaggttgcg gcgctcaggc gacacctggt gttcttcaat accacgccag ccgtatgtgg
    25201 cccggtcatc ggcgtcactg ccgccatgga agaggcgcgg gccaacggcg cggaaattga
    25261 tgacggtacc attaacggca tcaaagtcgg tctgatggga ccattggcag gggttggcga
    25321 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
    25381 ttcgggcaac attctcggcc cgctgctgtt cttctttatt ttcaacgcgg tgcgtctggc
    25441 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
    25501 gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg attctcggac tgtttgtgat
    25561 gggcgtgctg gtcaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgca
    25621 tgccgccgat ggctccaccg tcaccatgac cgtgcagaac attctcgacc aactttgccc
    25681 tggtttgctg gcgctcggtc tgacgctact aatggttcgt ctgctcaaca aaaaaattaa
    25741 cccggtatgg ctgattttcg ccctgtttgg cttagggatt atcggcaatg cgctgggctt
    25801 cctgtcctga ttcttcgccc cggcacgact gccggggcca tcgctcaaca tgaggtggtt
    25861 tatgaaaaca acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga
    25921 ccttcctgaa atgcaggagg atgaaatcct cgcgacggtg gtcactgaca gcctgtgcct
    25981 ctcttcctgg aaagaggcca acctgggtga aaaccataaa aaagtacccg acgatgtggc
    26041 gaccaaccca atcatcatcg gccacgagtt ttgcggcgat attctggccg tgggtaaaaa
    26101 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
    26161 cgaccgcccg gactgccccg gctactcctt cccgtgggta ggcggcgaag ccacgcatgt
    26221 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
    26281 ttttgaaggc tcgatggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
    26341 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
    26401 gatcctcggc ggcaccggac caatgggact gttggcgatt gattatgcgc tgcatggacc
    26461 cgttaacccg tcgctgctcg tcattaccga taccgacaac gataaattga gttatgcgcg
    26521 caagcactat ccatcagaat cgcaaacact gattcattat ctcaatgcca ccgatgcagc
    26581 atttgatacg ctaatggcgc tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
    26641 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgcctgaa
    26701 tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca attaatttct acgatgtgca
    26761 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcggc
    26821 ggtcaaactg atcgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
    26881 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct
    26941 ggtgtatacc gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact
    27001 ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatatctgct
    27061 cactcatgca gaggcaattt cccatgatta atcgcgttac tcaactctgc atgtcgctgg
    27121 ctggtcgcac cagcaacttc gcgaccggat gtgctgctga ttgctgaagc agcacaattt
    27181 gcacgagaag cataaaaaat atcagcgcct taaggggcgc tcaaattggt ggcaaagtgc
    27241 acattgttta tgccggatgc ggaatgaagc cttattcggt ctacataatt ttgaaaattc
    27301 aatatattgc agttatctgg taggccaggt aagcgtagcg catcaggcaa tgctgtgttt
    27361 gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt
    27421 aaattcgcag tggtagcatt ccttccgtga tttcttacta accggagaag taatggaact
    27481 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct
    27541 aattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac
    27601 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt cttgctccgc tgggtgtttc
    27661 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt
    27721 gattgtcggc ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
    27781 ggcaccaatt actgacgtgg ttaaacgtgg cgcactgtat attggttgga gcgcaggcgc
    27841 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
    27901 tttcgatgcg ctgaatctgt tcccgctgca aatcaacccg cacttcacca acgcgctgcc
    27961 ggaaggccat aaaggtgaaa cccgtgaaca gcgtattcgc gaactgctgg tcgtcgcgcc
    28021 agaactcaca attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc
    28081 aacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggttccgct
    28141 agaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
    28201 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
    28261 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
    28321 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
    28381 tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
    28441 tacgaaagcg atgcttgctg cggcgcaaac gacgggcgct ggtaacatca ccagagaaga
    28501 aaaccgacat tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct
    28561 tcaagccatc aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctcc
    28621 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
    28681 cccagcggcg cgactcttct tctgccagtt cctcttttgg catccgcgcc agatacagct
    28741 taatggcggt atagagaacg ttgatatcat cagccagctt acgcagctct ttctcctgcc
    28801 gtggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa
    28861 tacgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
    28921 catccagatg tttgggtcgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
    28981 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatggc caggcaacgt acaaggttgt
    29041 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacagcttc cccattgtct
    29101 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
    29161 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
    29221 ggccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc
    29281 ctgcggcggt cagagtcgca gtcagcagta cagcagcaag gctggagtag ctgataatgg
    29341 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
    29401 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
    29461 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
    29521 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
    29581 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc acccagcaca atcaccagag
    29641 ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
    29701 tactgctctg caccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttctttt
    29761 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
    29821 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
    29881 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
    29941 ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag
    30001 attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat
    30061 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
    30121 ccagattcgg tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat
    30181 agtcttcaac ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccggatgac
    30241 tgaaatacca acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca
    30301 tgccggtatg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
    30361 ggcaggccgg gtagcccggt gccggacgga tgccctgata gttttcgcgg atcagttctt
    30421 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
    30481 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
    30541 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
    30601 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
    30661 gacagtagtt agcgaatcct gttttttcgg tctgttgacg cagatggtgg ctgacgttga
    30721 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
    30781 ggaacagccc caccacacca cgcggattca gcgttttctc ggcgcttaat ttatccagca
    30841 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc taccacttca tcttccagaa
    30901 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggcgtccag tcgatgtaat
    30961 tacgcagcgt ttcgatgctg gcttcaactt cctgcacacc gagacggtgc gccaccggcg
    31021 gcgtgtaagc ctgccagtca aaagcgaaat cgttatcgcg cgccgtttcc agcgtgaccg
    31081 gtggtgtgcg tggtttcttg cgcccgtgct gaatacgcac ggtttcgtac tccttgcggg
    31141 tacgagcgac aaaatcatcg cgctgagtat cggaaagcag cgccgccacc acgccaacgg
    31201 tgcgcgaggc attctgcaca tacaccgtcg ggccgctgta gttctgctca attttcaccg
    31261 ccgtgtgcgc ttttgaggtg gtcgcgccgc caatcaatag cggaatggtg aagccctgac
    31321 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
    31381 ggccaatcaa atcagcattc acttctttag cagtacggag aattttttcc gcaggcacca
    31441 tcacgccgag atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt
    31501 taccgatatc gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc
    31561 cctgctcttt gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc
    31621 gcgccgattt gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca
    31681 cgttcatgcc gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
    31741 gggcttcttc ggtatcctgc tcgataaact cagtaatgcc tttgaccagc gagtattcca
    31801 gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg
    31861 ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcatcgcgac
    31921 gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg
    31981 ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
    32041 acaccgcgtg aatagcttcg cgcaccggat cgttaccacg gaacgagaac gagacgttgg
    32101 agacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga
    32161 taaagtcctg cgcatagttg ttgtgctctt caatgccggt ggcgaccgcg aagatgttcg
    32221 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    32281 gacggcaaat ctcgattttc cgtacgcggg tatcggcctg tccctgttcg tcaaaggcca
    32341 ttaccaccac ggccgcaccg taacgacgca acaatttcgc gtgatggata aaggcatcga
    32401 cgccctcttt catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    32461 tttcaatgac atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    32521 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat
    32581 ccatgttgat atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagcg
    32641 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttag
    32701 tccgctcgcc gacgttcaca aacaggctgt cgtcgccaat gttcagcggc tccaggccgg
    32761 acaaacggca ggctacggga atttcaggca gtttgcgcgg cgctaatcct tctactgcac
    32821 gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa
    32881 aacccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact
    32941 caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc
    33001 gtgacagctc ctgcacgtac tggcgcagtt catctggccc cagcgcacag ttcaggccaa
    33061 aggtcagcgc ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga
    33121 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    33181 gcgcttcaaa ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa
    33241 cggtttcaat caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat
    33301 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag
    33361 aaatagacgc cgtgcggttg gtcgggccga gaacaccggc aacgtagcgc ggtttctctg
    33421 gcgtgcgcgc ggtccactca tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga
    33481 tttccgccga cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg
    33541 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    33601 cttccggttt gcttagcacc agcagatcgt tattgccttt gaggtcgcat ggccagtcgg
    33661 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    33721 taccgccgtc cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    33781 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    33841 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    33901 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt
    33961 ccacgataca gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa
    34021 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    34081 aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacggag ctggcgcaac
    34141 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    34201 tcgtgcgtca ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg
    34261 gcagcagctt tctccagagc cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc
    34321 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    34381 cgattattat cgaccaggta cagtgtaccc acctgatgcg aatgtccgcg cctatcggcg
    34441 gtaaattacc tatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    34501 aacaggtgac gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg
    34561 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    34621 acgatgagga acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    34681 gtgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg
    34741 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    34801 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccgtcata cctgagtggt
    34861 aatagaataa gggtgtctgt taatcgcatt gacgccaaaa taacttattg tcatacactt
    34921 cacacacttt tattaactca gcattatttt taaacatcaa accacttaat tattaacaaa
    34981 atcgcaagat ctcattccac cgaaactttt acggctgctc aatgagattg agcgatttat
    35041 gatatttctt tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa
    35101 gacatcaata aacaatgaga tatttaaatg attactcgta ttcctcgcag ttctttctct
    35161 gtaaatatta ataatacagc ccagacaaat gaacaccaaa acctgagtga attgttttat
    35221 aaagaactcg aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc
    35281 tcagagaact gtagacaaaa cggtcgtcat atctttagca acaaggattt tgtcattaaa
    35341 tt
//
LOCUS       DAZGCQ010000048        35336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000048 DAZGCQ010000000
VERSION     DAZGCQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.048"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..288)
                     /locus_tag="ACHK07_002516"
                     /pseudo
     CDS             complement(1..288)
                     /locus_tag="ACHK07_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            561..1355
                     /gene="yjfZ"
                     /locus_tag="ACHK07_002517"
     CDS             561..1355
                     /gene="yjfZ"
                     /locus_tag="ACHK07_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO8102833.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLAKKCNAYDGVTMNGDNSFGWNHETTLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(1426..1875)
                     /gene="rplI"
                     /locus_tag="ACHK07_002518"
     CDS             complement(1426..1875)
                     /gene="rplI"
                     /locus_tag="ACHK07_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO8102834.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1917..2144)
                     /gene="rpsR"
                     /locus_tag="ACHK07_002519"
     CDS             complement(1917..2144)
                     /gene="rpsR"
                     /locus_tag="ACHK07_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO8102835.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2149..2463)
                     /gene="priB"
                     /locus_tag="ACHK07_002520"
     CDS             complement(2149..2463)
                     /gene="priB"
                     /locus_tag="ACHK07_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO8102836.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2470..2865)
                     /gene="rpsF"
                     /locus_tag="ACHK07_002521"
     CDS             complement(2470..2865)
                     /gene="rpsF"
                     /locus_tag="ACHK07_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO8102837.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3192..3467
                     /gene="yjfY"
                     /locus_tag="ACHK07_002522"
     CDS             3192..3467
                     /gene="yjfY"
                     /locus_tag="ACHK07_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO8102838.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3596..4282)
                     /gene="ulaF"
                     /locus_tag="ACHK07_002523"
     CDS             complement(3596..4282)
                     /gene="ulaF"
                     /locus_tag="ACHK07_002523"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO8102839.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4282..5136)
                     /gene="ulaE"
                     /locus_tag="ACHK07_002524"
     CDS             complement(4282..5136)
                     /gene="ulaE"
                     /locus_tag="ACHK07_002524"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO8102840.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5146..5796)
                     /gene="ulaD"
                     /locus_tag="ACHK07_002525"
     CDS             complement(5146..5796)
                     /gene="ulaD"
                     /locus_tag="ACHK07_002525"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8102841.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5810..6274)
                     /gene="ulaC"
                     /locus_tag="ACHK07_002526"
     CDS             complement(5810..6274)
                     /gene="ulaC"
                     /locus_tag="ACHK07_002526"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO8102842.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6284..6589)
                     /gene="ulaB"
                     /locus_tag="ACHK07_002527"
     CDS             complement(6284..6589)
                     /gene="ulaB"
                     /locus_tag="ACHK07_002527"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO8102843.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQYLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6605..8002)
                     /gene="ulaA"
                     /locus_tag="ACHK07_002528"
     CDS             complement(6605..8002)
                     /gene="ulaA"
                     /locus_tag="ACHK07_002528"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8102844.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8357..9421
                     /gene="ulaG"
                     /locus_tag="ACHK07_002529"
     CDS             8357..9421
                     /gene="ulaG"
                     /locus_tag="ACHK07_002529"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO8102845.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9529..10284
                     /gene="ulaR"
                     /locus_tag="ACHK07_002530"
     CDS             9529..10284
                     /gene="ulaR"
                     /locus_tag="ACHK07_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO8102846.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10281..11030)
                     /gene="yjfP"
                     /locus_tag="ACHK07_002531"
     CDS             complement(10281..11030)
                     /gene="yjfP"
                     /locus_tag="ACHK07_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8102847.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11212..11541
                     /gene="bsmA"
                     /locus_tag="ACHK07_002532"
     CDS             11212..11541
                     /gene="bsmA"
                     /locus_tag="ACHK07_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO8102848.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11690..11965
                     /gene="yjfN"
                     /locus_tag="ACHK07_002533"
     CDS             11690..11965
                     /gene="yjfN"
                     /locus_tag="ACHK07_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO8102849.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12082..13707)
                     /gene="aidB"
                     /locus_tag="ACHK07_002534"
     CDS             complement(12082..13707)
                     /gene="aidB"
                     /locus_tag="ACHK07_002534"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO8102850.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13791..14954)
                     /gene="yjfC"
                     /locus_tag="ACHK07_002535"
     CDS             complement(13791..14954)
                     /gene="yjfC"
                     /locus_tag="ACHK07_002535"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8102851.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14957..15595)
                     /gene="yjfM"
                     /locus_tag="ACHK07_002536"
     CDS             complement(14957..15595)
                     /gene="yjfM"
                     /locus_tag="ACHK07_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO8102852.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15605..16003)
                     /gene="yjfL"
                     /locus_tag="ACHK07_002537"
     CDS             complement(15605..16003)
                     /gene="yjfL"
                     /locus_tag="ACHK07_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO8102853.1"
                     /translation="MHILDSLLAFSAYFFIGVVMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16021..16680)
                     /gene="yjfK"
                     /locus_tag="ACHK07_002538"
     CDS             complement(16021..16680)
                     /gene="yjfK"
                     /locus_tag="ACHK07_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO8102854.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16731..17429)
                     /gene="yjfJ"
                     /locus_tag="ACHK07_002539"
     CDS             complement(16731..17429)
                     /gene="yjfJ"
                     /locus_tag="ACHK07_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO8102855.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17448..17849)
                     /gene="yjfI"
                     /locus_tag="ACHK07_002540"
     CDS             complement(17448..17849)
                     /gene="yjfI"
                     /locus_tag="ACHK07_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO8102856.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSDNALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEIASLVDNALDLAEITEEYSH"
     gene            complement(17976..18707)
                     /gene="rlmB"
                     /locus_tag="ACHK07_002541"
     CDS             complement(17976..18707)
                     /gene="rlmB"
                     /locus_tag="ACHK07_002541"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO8102857.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18888..21329)
                     /gene="rnr"
                     /locus_tag="ACHK07_002542"
     CDS             complement(18888..21329)
                     /gene="rnr"
                     /locus_tag="ACHK07_002542"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO8102858.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21368..21793)
                     /gene="nsrR"
                     /locus_tag="ACHK07_002543"
     CDS             complement(21368..21793)
                     /gene="nsrR"
                     /locus_tag="ACHK07_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO8102859.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21998..23296)
                     /gene="purA"
                     /locus_tag="ACHK07_002544"
     CDS             complement(21998..23296)
                     /gene="purA"
                     /locus_tag="ACHK07_002544"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO8102860.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKERSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23400..23597)
                     /gene="yjeT"
                     /locus_tag="ACHK07_002545"
     CDS             complement(23400..23597)
                     /gene="yjeT"
                     /locus_tag="ACHK07_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO8102861.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23679..24683)
                     /gene="hflC"
                     /locus_tag="ACHK07_002546"
     CDS             complement(23679..24683)
                     /gene="hflC"
                     /locus_tag="ACHK07_002546"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO8102862.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24686..25945)
                     /gene="hflK"
                     /locus_tag="ACHK07_002547"
     CDS             complement(24686..25945)
                     /gene="hflK"
                     /locus_tag="ACHK07_002547"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO8102863.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(26031..27311)
                     /gene="hflX"
                     /locus_tag="ACHK07_002548"
     CDS             complement(26031..27311)
                     /gene="hflX"
                     /locus_tag="ACHK07_002548"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO8102864.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27388..27696)
                     /gene="hfq"
                     /locus_tag="ACHK07_002549"
     CDS             complement(27388..27696)
                     /gene="hfq"
                     /locus_tag="ACHK07_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO8102865.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27782..28732)
                     /gene="miaA"
                     /locus_tag="ACHK07_002550"
     CDS             complement(27782..28732)
                     /gene="miaA"
                     /locus_tag="ACHK07_002550"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO8102866.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28725..30572)
                     /gene="mutL"
                     /locus_tag="ACHK07_002551"
     CDS             complement(28725..30572)
                     /gene="mutL"
                     /locus_tag="ACHK07_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO8102867.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPTPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPHEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30582..31919)
                     /gene="amiB"
                     /locus_tag="ACHK07_002552"
     CDS             complement(30582..31919)
                     /gene="amiB"
                     /locus_tag="ACHK07_002552"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO8102868.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31938..32399)
                     /gene="tsaE"
                     /locus_tag="ACHK07_002553"
     CDS             complement(31938..32399)
                     /gene="tsaE"
                     /locus_tag="ACHK07_002553"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO8102869.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32371..33918)
                     /gene="nnr"
                     /locus_tag="ACHK07_002554"
     CDS             complement(32371..33918)
                     /gene="nnr"
                     /locus_tag="ACHK07_002554"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO8102870.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIASLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTEKNHD
                     ESSNSAP"
     gene            33917..35056
                     /gene="queG"
                     /locus_tag="ACHK07_002555"
     CDS             33917..35056
                     /gene="queG"
                     /locus_tag="ACHK07_002555"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO8102871.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(35039..35092)
                     /locus_tag="ACHK07_002556"
     CDS             complement(35039..35092)
                     /locus_tag="ACHK07_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102872.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8552 a         9706 c         8614 g         8464 t
ORIGIN      
        1 caggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
       61 cacctgaaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt catccaataa
      121 ggattccatt aactgcaagt gcgcatgggt aatttctaag agacgctgtg attttgcata
      181 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccagg gattagcaat
      241 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggct ggctaacctg
      301 ctgattaacg aatacaccga taccgacagc gtagaataat tacataccgc acaacaagaa
      361 tgtacggtgt aaggcgtgga agtcattttc tccccgggtg catcgttata tccataatga
      421 gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca gaaaacaaac
      481 ttatttttat taatatcaat tctttttctt tgaacattca actgcgatat ttgtaaaaat
      541 attaaacagg caacaataaa atgacattac caacaactat ttattcattc cccgcatatt
      601 tacctcgttt ttcgtccacc gacaaaccag caaaactaaa atttcatcaa tacgcacgcg
      661 ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt actgttgaga
      721 tccacaaact agatctctct gactggcaag cattcaataa gttagcaaag aagtgtaatg
      781 cttacgatgg agtaacaatg aatggtgata attcttttgg atggaaccat gaaactacat
      841 tagataatat tcacgcccag aaatacaata aagcttacgc gggtgcgagg ttaaccgcag
      901 aactgaaata tttattgcaa gatgtcgaat ccttcgaacc caacagtaaa tacacaatac
      961 acgaagtggt tttgggacct ggttatggta ctccagacta taccggccaa acgataggat
     1021 acgttgtaac ccttcctgcc caaatgccta attgctggtc atctgaacta ccaacaatcg
     1081 atctttatat cgaccaactt cgcactgtta caggcgtttc taatgccctc ggattcatca
     1141 ttgctgcatt gctaaacgcg tattctgatt taccccatga tttgaaaatt ggtctgcgtt
     1201 ccttatcaag ttcagccgca atctactcgg gtttgggttt cgagcgagtg ccgcaggagc
     1261 gagacatcag ttgcgctcgt atgtatctaa cgccagcaaa ccatcctgac ttatggacac
     1321 aagagaatgg tgaatggatc tacttgcgaa actaatacct gacgaaaaat agaaagcaaa
     1381 acgccgacca atggtcggcg tttttacgtc tcgttgaata acgaattatt cagctactac
     1441 gtttacgatc actttcgcga atacttcgct gtgaacctgg aagctcactt cgtgttcgcc
     1501 agtggtacgc agaacgccgt tcggcagacg aacttcgctc ttagccactt caacgccagc
     1561 tgcagttaca gcgtcagcga tgtcgcgagt accgatggaa ccgaacagtt taccttcgtc
     1621 gccagcttta gacgcgatgg taacagtttc cagtgcattg attttctcag cgcgagcatt
     1681 agctgctgcc agaacttcag ccagtttagc ttccagttca gcgcgacgtg cttcgaagaa
     1741 ttcaatgttt ttcttggtag ctggaacagc tttaccctgc ggtaccagga agttacgagc
     1801 atagcccgct ttaacgttta cctgatcacc caggctaccc aggtttgcta ctttatcaag
     1861 cagaataact tgcattacct tatcctctca aagtcgtatt aatggaccgt gaccgattac
     1921 tgatggcgat cagtgtacgg cagcagggac aggtagcgag cgcgtttgat agcgcgagcc
     1981 agctgacgct ggtattttgc acgggtaccg gtgatacggc ttgggacaat cttaccgctt
     2041 tcggtgatgt agtttttcag cgtagcgata tctttatagt cgatctcttg aacgccttcc
     2101 gcggtgaaac ggcagaactt gcgacgacgg aaataacgtg ccatatggct agtctccaga
     2161 atctatcaat tcaatctgct cggcatgcaa aaccattttg ctcagtccgt tctttgcctt
     2221 gtggcatgaa atgaacccct gaacggttat gcgactgccg accgttatac tgtgagtaat
     2281 ggcctggttt tcgtgtccgc taacaataac gggcatttga caccatgcct gccggtgaaa
     2341 gccggcttcc tcctgcacag aacgatgctc aagcacgaac tggcagtgag gaattcctga
     2401 tggactgacc tttcgaaggg gagtcctgca cacggtgccg gacaacacca gacggttggt
     2461 catcagaaat tactcttcag aatccccagc ttcagcatca tcagcggttt cgtttgcgaa
     2521 atcatcgcga cgctcacggc gctcgtcttt cgctttaacc atcggagatg cttcggtaac
     2581 agcgtgcttg gtacgcataa ccatgctgcg gataacggca tcgttgaagc ggaaggtagt
     2641 ttccagctca tcgatcactt cctgcggagc ttcaacgttc atcagaacgt agtgagcttt
     2701 gtgcagtttg ttgatcgggt aagccagctg acggcggccc cagtcttcca gacggtggat
     2761 cttgccttct gcaccagtga tggcagcagt gtagcgctcg atcatgcccg gaacctgttc
     2821 gctctggtca ggatggacca taaaaacgat ttcgtaatga cgcatcgaat tgctccttac
     2881 ggattattca gcctcctgtc tgggtcagcc gaatcccggg gaggcaagga acgtgttaaa
     2941 ggtcggctga aaaatgacgc gttatagtac tgacataccc cgctaaactc aaggccagcc
     3001 gacaaataaa tctcatcttt caccaaagtg gactaacacg ctgatttgaa gttcatcaca
     3061 cttcatcatg gtgttttttt gaacaactgc atcagaaatg gtcaccatca acttctttat
     3121 cattgattaa aattaaatca gaagatcatc acagaggaga gcacacaaag tcagccgtcg
     3181 aggaggtatc tatgttcagt cgtgttttag cccttctggc tgtgcttttg ctaagtgcaa
     3241 atacatgggc agccattgaa attaataacc accaggccag aaatatggac gatgtgcaaa
     3301 gcttaggcgt gatttatatc aatcataatt tcgccactga aagcgaagca cgtcaggcat
     3361 taaatgaaga aacagatgcg caaggcgcaa cgtactacca cgtaattctg atgcgggaac
     3421 cggggagtaa cggcaatatg cacgccagcg cggatattta tcgctagcac caggtataac
     3481 caacgaaaca ttgccatagt ttgctttgcc cccttcgcag ggggcttttt ttgaaaaacg
     3541 gctgcggaat tagaccagtt atctcccgag gaaggaaatt tccgcagcgc gtgttctact
     3601 tctgcccgta ataagcgtta ggaccgtgtt tacgcatgaa gtgtttattc atcaggaagc
     3661 tgtcgatgtg attcagttgt gggttaatgc cgcgcgcaat ccacgccatt ttcgccactt
     3721 cttccatcac caccgcgtta tgcaccgcat cgtgggcatc tttcccccag gcgaacggtc
     3781 cgtgctgata caccacaatt cccggcgtat gcagcggctc ggcgttgccc agcgtttcga
     3841 taatcacttt gccggtgttc agttcatact cgccctgcac ctcttcttcg cttaacccgc
     3901 gcgtacacgg aatgtcgcca aagaagtagt cagcgtgcgt ggtgcctaac gccgggatcg
     3961 ccagccccgc ctgcgcccat gcggtggcat gagtggagtg ggtatggaca atgccgccaa
     4021 gcgacgggta acgacggtag agttcgagat gcgtcgcagt gtcggaagat gggcgatact
     4081 ccccttccac caccttgccg ctcatatcaa ccaccaccat atcggccgct ttcatggttt
     4141 cgtaggcaac gccgctgggc ttgatcacca ccagcccgcg ttcgcggtcg atagcgctga
     4201 cgttgcccca ggtaaaggtc accagcccgt agcgcggcag ctccatgttg gcttcaaata
     4261 cctgctgttt tagcttttgc attatgccgc ctccaccatg cccgctttcg ccatgcgcgc
     4321 tttcacccaa tcacgcgctt tcgccacttc tgccgccggg tcttccgccg tttcgctcca
     4381 catctcaatc aggtacggcc cgcaatagcc actctgtttg agcgtttcga aacaacgttc
     4441 gaaatccact acaccttcgc caaacggcac gtttttgaag acgcccggtt tggtgtcttt
     4501 cacatgcacc gcgacgatat gcccgattcc ggcctgcaac tccatctgca cgtcgttatc
     4561 ccacgccgac aggttgccga tatccgggta gagctggaac cacggattgt tgagatagtg
     4621 cgcgtagccc agcgccttgc tgatggagtt catcaacgga taatccatga tctccatcgc
     4681 cagcgtcacc tgcgcgcggc ttgccatctc aacgctctct ttcaggccgt cacggaaacg
     4741 acgacgcgtt tcgttattgg cttcctgata gtaaacgtca tagcccgcca gctggatcac
     4801 gcgaataccg acatcctggg cgaactggat agctttacgc ataatctcca gcccctgcgc
     4861 ccgcaccgcg tcatcttcac tgcccagcgg gaaacgacga tgagcagaaa ggcacatgga
     4921 cggcacgcgc acgccggttt caacaatcgc attgaccagc gccagacgct gctcgcggct
     4981 ccagtcgagg cgcgacaggc gctcgtccgt ttcatctacc gacatctcga caaaatcgaa
     5041 gcccaacgtt tttgccagtt gcaggcgttc cagccagcac tccccggcgg ggagcgcttt
     5101 ttcatagatg ccaagcggga tttgtttgga caacatatcc gctccttagc cccacagttc
     5161 agcgatggaa cgtttgaact gacgtgcggc ttccaccgga gaagcggcat cacggatact
     5221 acggcccgcg ataaagacgt gaatcggaat acccttgaac agcggcagat cttccagcgc
     5281 caggcctccg gtgacggtga ctttgaagcc catatcggaa agacgtttga tcgcggtgat
     5341 gtccgcttcg ccccacgcca cgcctgcggc ctgcgcgtca cggctgcggt gataaaccac
     5401 ctgctgaatg cctgcatcgc gccactgttg cgcctgttcc caggtccagt aaccggtcag
     5461 ttcgatctgc acgtcgccgt taaactcttt tgccacgtcc agcgcgcctt tggcggtgtt
     5521 gatatccgca cagcaaatta ccgtcaccca gtcagcgttg gcttcgaagc acatacgcga
     5581 aaggattttg cccgcatcgg caattttggc gtctgccagt acgattttgt gcgggtaaag
     5641 cgctttcaga tcgcgaaccg cacgcacgcc ttcgcccacg cacagaatgg tacccacttc
     5701 gataatgtcg acttcttcgg caatcaggcg agtggtttcg taggcgctat ccatagtctg
     5761 gttgtccagc gcgacttgca acatcggtaa tgacatgttc aattccttct taagctgccg
     5821 cgttggtgcg gtcaatgaga tccagtactt cctgctcggt acggcaggcg cgtaaacggt
     5881 caaaattctc ttcatcttca aacaggttga cgatctgcat gatgcccact tcctggtgag
     5941 tattggcatc gaccgccgcc atggtgatga ggatatccac cggatcgttg tcgtcgtgat
     6001 taaattccag cggctttttc agcgttacca gagagaaacc tgttttctta acaccctctt
     6061 ccggccgccc gtgcggcatc gccagccccg gagcgataac gaaataaggg ccaaactgtt
     6121 ccacgccatc cagaatcgcc tggtagtaac gcggctcgac aacatccgcc gccaccagca
     6181 ggtcaacgcc gatttttact gcgtcctgcc atgtctctgc ttcagcctgc aggcggatag
     6241 atttattctc cgccagcgaa tcacgtagtt tcatggcgta tccttacttc acgtcctgcg
     6301 ggaaatgctc tttgatcact tccagcagtt tcgggccaaa gtcggcagga gagagcatgt
     6361 tgcgcacgcc aaccacatat ttgttgccgg taacggtgat ttcgcccgcg atgtgcgtag
     6421 aagcgatgat gatatccgcg ccgctcaact cgcttttgta ctcgccaacc gcgcagctgt
     6481 ttaccgtgtg gtcaatgttt gattgggtta aatactggtc cactttcatt ttcatgatca
     6541 tggaactgcc ttgtccgttg ccacacacag ccagaatacg tacggtcata atcaaaactc
     6601 cttattaagc agactgttct gccagttgtt tttctgcatc ttcttctgcg cgcagtgcgc
     6661 ggccagcgaa gaacatataa gccagtgcaa ttacaatgat gacggccata aaggcgatac
     6721 cgatggagaa gaagccttgc atcatcggcg gtgccagaat cgaccagtcc gccatgccca
     6781 tccaggcact cataccggtg agtttcaccg cccagacgca accaaagatt tcgatcattc
     6841 ccatcaccag acaaatcttc agcgctgcac gccagccgcc gaagtggtta gcgaacacgc
     6901 cgatggtggc gttggagaag aacatcggga taaagcctag aataatcaga atggaagagc
     6961 cgcaggcgac caggatgcca actgcaatca gctgaccgat ggtgccccac ataaagcccc
     7021 agaccacggc gttcggcgcg aagctataga tagccgcaca gtcaatcgcc agaaccgcac
     7081 ctgggatcag gcgctgggag ataccgttaa atgcttcaga gagttccgcc acaaacatgc
     7141 gcacgccctg agtgatgatg aagatcgcca ccgcgaagga gaaaccggtt tgcaggatgt
     7201 agaccgtcca gtgcactttg cccgccatcg cctgcacggt gtcgataccg aaggagagca
     7261 gaatggcgcc aaagaagatg gtcatcacaa tcgccgtgga gacgatgttg tcgtggaaaa
     7321 tgttcagcca gcccggcaat ttgaggtctt caacgctctc ctcttttttg ccgaggaacg
     7381 gtgcgacttt ataggcaatc catgatgcaa actgctgctg gtgaccgatg gagaaaccac
     7441 agccatccgt cacttcctga gtcggcttgt acatcatgtt ggaagtgatg ccccagtaga
     7501 gcgaaaccag aatcgcggta cagataatgg tggtccacat ggagtagccg aagataaaca
     7561 gcgtaacggc aatcaacccg gcctgctgga acatgatgtg gccggtcaac atgattgtgc
     7621 gaatgccggt aatgcgacgc agcagcacgt aacagatgtt cagcgccagc gctaacaata
     7681 cggcgtaacc cacccagcta taggcatcgc ccatgcggtc gatggttgcc atcatcgaag
     7741 cgtaagtatc tgaaattgcg ccgttaatgc cgtagacttc ggacattttc gccaccaccg
     7801 gtttgaaggt gctggtgagg atgccggacc ctgcctgcaa caacatgaaa ccaattatgg
     7861 ttttaatcgt gcctttaata ataacgctga cacttttgcg cagtaggatg tagcccagac
     7921 aggtcacaat acccagcaac aacggggcat tggtcatgac ctggttaaaa aacacggtaa
     7981 agatgttgta gaggatctcc ataacgatct cctgaagaag aggtaaccgg gtattcctca
     8041 cacccggaat gttgtgcact cactctaatt ttcaaaagta atcacaacaa gattatttat
     8101 gattaaatgt gacgcgacac acaaataatt ccataacgaa aatatgggat tcacacaaag
     8161 aaacaattaa tcatttaaat tcattaagtt aattgaatta cacattccat taaatctttc
     8221 catactccaa attcacaaca aatcacccgg caaaaagtca aaaaatggtc attgccttcc
     8281 tgaaaaaata gtgtcaggat taatcaaaac caatcatatg ttgattcgaa ttgaactatc
     8341 atgaaggtaa atggcgatga gtaaagtgaa aagtatcacc cgtgaatcct ggatcctgag
     8401 cactttcccg gagtggggta gctggttgaa tgaagaaatt gaacaagagc aggtagctcc
     8461 tggtacattt gcgatgtggt ggcttggctg caccgggatc tggctgaaat cggaaggtgg
     8521 caccaacgtt tgcgttgatt tctggtgcgg cacaggcaaa caaagtcacg gtaacccgtt
     8581 aatgaaacag ggtcaccaga tgcagcgtat ggctggcgtg aaaaaactgc agccgaacct
     8641 gcgcaccacc ccgtttgttc ttgatccatt tgctattcgc cagatcgacg ccgtcctggc
     8701 gactcacgat cacaacgatc atatcgacgt taacgtcgct gctgccgtga tgcagaactg
     8761 tgctgatgac gtaccgttta tcggaccgaa aacctgtgtg gatttatgga tcggctgggg
     8821 cgtgccgaaa gaacgttgca tcgtggttaa accgggcgac gtggtgaaag tcaaagacat
     8881 tgaaattcat gcgcttgatg ctttcgaccg tactgcactg atcaccctgc ctgccgatca
     8941 aaaagcggct ggcgtactgc cagatggcat ggacgatcgc gcggtgaact acctgttcaa
     9001 aacgcctggc ggctccctgt atcacagcgg cgactcccac tactctaact attatgcgaa
     9061 gcacggtaac gaacatcaga tcgacgtggc gttaggatcg tacggcgaaa acccgcgcgg
     9121 tatcaccgac aaaatgacca gcgccgatat gctgcgtatg ggcgaagcgc tgaatgcgaa
     9181 agtagtgatc ccgttccacc acgatatctg gtcaaacttc caggccgatc cgcaagagat
     9241 ccgcgtgctg tgggagatga aaaaagatcg cctgaaatat ggctttaagc cgtttatctg
     9301 gcaggtgggc ggcaaattta cctggccgct ggataaagac aacttcgagt accactatcc
     9361 gcgcggtttc gatgattgct tcactattga accggatctg ccgttcaagt cattcctgta
     9421 atctgtcgtt tataccggat agcgtggaga agttcgcgct atccggcgta gtatttcctg
     9481 gctatttcaa atatcatcta aaaaaatcaa attttatcgg aatagctcat gactgaagca
     9541 caaagacatc aaatcctcct ggaaatgctc gcacaattgg gctttgtgac cgttgagaaa
     9601 gtcgttgagc gtctgggaat ttcgcctgcc actgcgcgac gcgatatcaa taaacttgac
     9661 gaaagcggca aactgaaaaa agtgcgcaat ggcgcagaag ctattaccca acagcgcccg
     9721 cgctggacgc cgatgaatct gcatcaggcg cagaatcacg atgaaaaagt acgtattgct
     9781 aaagcggcct cgcagctggt taatccgggc gaaagcgtag tcatcaactg cggctccacc
     9841 gcgtttctgc ttgggcggga aatgtgtggc aagccagtgc aaatcatcac taattatcta
     9901 ccgctggcaa attacctgat cgatcaagaa catgacagcg tgatcattat gggcggacag
     9961 tacaacaaaa gtcagtccat cactttaagc ccgcagggca gcgaaaacag tctctatgcc
    10021 gggcactgga tgtttaccag cggaaaaggg ctgaccgcag aagggttgta taaaaccgat
    10081 atgctgacag caatggcaga gcagaagatg ctgagcgtgg tagggaaact ggtggtactg
    10141 gttgatagca gtaagattgg cgaacgcgcg ggaatgcttt ttagccgtgc cgatcaaatc
    10201 gatatgctta tcaccggcaa aaatgctaac ccggaaatcc tgcaacaact ggaagcgcaa
    10261 ggggtcagca ttctgcgtgt ttaaagatgc tggcggaaaa atgtcacggc agcatctaac
    10321 gcctcaggcg taatgcggtg acgcacgcct ggctgccatg aacaggttag cagtttatcc
    10381 cgtcccgtct cgcttaaggc ctgctgcaaa cgtagtgatt catcggcagg cacaacgtca
    10441 tcgtcgaggc catgccacag cagtagaggt ctgtccccaa gttgttccag gtggtttgtc
    10501 gcttcccact ctgccagcgg cgcgacaatg ttattgaatt cattctgctg tgctgccgtt
    10561 tcaggtatca gcggtggaaa cagtgaacgg gcgagcgatg taaaatagcc cgatcccatc
    10621 atgctggcgg tacatttcac cgtggggtgg cgagcggtaa ttcccagtgc cgtcatcgcg
    10681 cccatcgacg cgccaccgac tgccagacgg tcatcaagca gccagttttc ttcggctatt
    10741 gccgcacgta aagtagtgaa ttcctgcata ctttgtagca agatttgcca gaattgattt
    10801 aaccgccgcg ctgcgtcgcc actaaaacga ctaccgtgat cgggcgcatc cggcatgatc
    10861 acccgcaaac cagcctgcgc cagcgcaacg gcaaaatagc tatacaccag actggacgaa
    10921 gtaaagccgt gataaaaaat cacgcacggt aacggggtat ctttttgtcc gacaggataa
    10981 gcatgaagaa cgggaatatc tgccagctcg cgtgattcta tttcaatcat ctgtctctcc
    11041 tttttgtctg gtatgacatg aagaaatcct tagcacaagt ttctgctggc ggtattagtt
    11101 cataatgttg agatgtgggt tacgctttcg tcacgttttc attcgaaaat cgcgtaccag
    11161 gtaacaattc gggaacattc ccccaaagtt aaattaataa ggcactacac tatggttagc
    11221 aggaaacgta atagcgttat ttaccggttt gccagtttat tattggtgtt gatgttaagt
    11281 gcctgtagcg cactgcaagg tacgccacag ccagcaccgc cagtaacgga ccatccgcaa
    11341 gagattcgcc gcgaccagac gcaagggtta cagcgaatag gtagcgtaag cacaatggtt
    11401 cggggttctc cggatgacgc attagcagaa atcagagcaa aagctgtcgc tgcaaaagct
    11461 gattattacg ttgtcgtcat ggtggacgaa accatcgtga caggacagtg gtattcacaa
    11521 gccattttgt accgtaaata aatcagacaa cttacatagt cagctttaca ttgctttgcg
    11581 tccctatgcg tttacctgtg cacaataaat taccggctgg aagcctgggc gtttattacc
    11641 gcgatggaat gccctgcaac atgtggggaa acgaaaaatg gagctgacga tgaaacaatt
    11701 acttgcctca ccctcgctgc aattagtgac ttatcctgcg agcgccacgg cacagtctgc
    11761 agaattcgcc agtgctgact gcgtgacggg cctgaatgaa ataggtcaga tctcagttag
    11821 caatatttca ggggatccgc aggacgtgga acgtatcgtg gcgttaaagg ccgatgaaca
    11881 aggtgcgtca tggtaccgca ttattaccat gtacgaagac cagcagcctg ataactggcg
    11941 cgtacaggcg atactgtatg catgagtgtc atctacagca aatgaacaat gccggatgcg
    12001 gcgcgagcgc cttgtccggc ctacaatttt cacatatttc attgagttaa tcgaaaccag
    12061 cgtcgcatca gtcgtatacg cttacacaca cactcccccc gtcgcccgca gcaataaatc
    12121 attctggatc tgctctgaca accgtacgcc gccgcgcgta tctaacatca cctgacacca
    12181 cgcctgcgcc attggcggag aggcatattt caacatttgc gcaccgcagc ccagcaggaa
    12241 tagctgatga gtaatctctc gccccagttc ttcagctggc ttacgcagct gctgctgtaa
    12301 acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc acttccacaa atgcttccga
    12361 caataagtcg tatacgcccg cttgcttatt gagaacgcgc agcacatcca ggcacataat
    12421 attgccggaa ccttcccaaa tactgtttac cggcatctcc cggtaaagcc gcggcagctc
    12481 gctctcctcg caataaccaa tgccgcccag cacctccatc gcttcggcca caaacggcat
    12541 acctcgtttg cagatcacaa attttgccgc aggcgtaaat aaacgcgccc acagggcttc
    12601 tttggcatcg gctcgccggt cccacgctcg cgcaagacga aacagcaacg ccgtttgccc
    12661 ttcaagctga agtgccatgc gacttaagac atgacgcata aggggctgtt ggatcaatgg
    12721 attaccaaaa acatggcgtt gatgcgcatg ataaatcgcc agcgagaacg cacggcgcat
    12781 catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc ccccccattt tcaggatcag
    12841 acgaattcct tccccttcct gccccaataa ccagccaatg gcatcctgaa actccacttc
    12901 acaactggcg ttagagcgat tacccagctt atctttcagc cgctcgaggc gaatcgcgtt
    12961 gcgttgcccg tcaggcaaaa agcgcggcac aaaaaagcag gacaaaccgc ccgcggtctg
    13021 cgccagcacc agatgcgcat cgctttgcgg aaccgagaaa aaccatttat gccccaccag
    13081 ccgataagag ccatcctcca gacgctctgc gcgggtggtg ttgctcatga catcggaacc
    13141 gccctgcttt tccgtcattc ccatgccaat caacaaaccg cgtttttgcc cacctggcaa
    13201 taagtgagaa tcgtagcgat cgctcaacag cggcgtggtc cagtcctgaa acggcgtggg
    13261 taacatctgt aacaacaatg gcgtggcggc aaaggtcatc gttattggac ataacgaccc
    13321 cgcctcaacc tgtgcgtgca acataaaacg cgccgcgcgc gccacaaatg cgccggagcg
    13381 agcgtcttct tcccaggcaa gattgtgcac ccgattggta catagcgcct gcatcagcag
    13441 gtgccaggcg gggtgaaaac gcacatcgtc cagacggcgt ccttgcgcat cgtagcgcaa
    13501 taattcaggc ggattcacat tcgccagccg ccccagttca agggattcag ccgttcctaa
    13561 ctgctgacca atactggcta gaaaatcgct atcccagcca gcaccttcac gcgttaccgc
    13621 ttcgcaaagc gcgccatcag acaggtataa gttgctgtta tttaatggta taggttgatt
    13681 aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc atcaatggca gtcaccatta
    13741 gtatggtcac tgccatggat tcatgacaga aagagattgc taaaacattc ttatccagca
    13801 atgtaatgcg gaacgaaacg tgaggtgtct ttggtgatca gtgtgttatc ttcacgaatc
    13861 cccattccgc acgcttcatc atcgacaatc cagctaccga tgagtgtgta gctatcgcca
    13921 aaccgcggca gaggttgaaa cgcctggtag atcatcggtt catcggcgta atcaccatca
    13981 gcgtggtcaa cgacgttatt ctggccgtcg aaaatggtga catttccgcc ttcgcgcgag
    14041 tagatcggtt tacgcacata gctttcgcca gcggcaatct gcggtttttc accctcaaac
    14101 caggatgcaa gaaggttagg atgaccaggg aagaagcgcc aaagcagcgg cattagccct
    14161 ttattactca agatactttt ccataacggc tccacccatt gctcgcgacg cttgcgcagc
    14221 agcggaccgt tatcgtcacg catcatccac tccagcggat acagcttaaa tgcacgctgg
    14281 atgacattat catcaagatc ggtcagtacg ccgccgacgc ccaaaccgag atcttcaatg
    14341 tagataaacc gcgactcctg ccctgcctgc tgggcgcagt cctgcaagta cagcacggta
    14401 gtcctgtctt catcggtgtc ctgacagcag cagaaataaa acggctcccg actgtaaagc
    14461 tcactaaagc gcgaaatcag gcgttcctga atggcattgt actgatcggc atcacgcgga
    14521 ataatgccgc tgcgccgggc atcctctagc cacaaccact ggaaataagc cgactcatac
    14581 aacgaggtcg gcgtatcggc gttgtactcc agcagcttca ccggcgcatt gccacaccag
    14641 gcaaaatcca tccggccata cagcgaagga tcgcgtgcgc gccagctttc agcgatcaca
    14701 tcccagtaca acggagggat cgccagttgc gtcaggatct cttcgtcttt aaccgcgcga
    14761 tccaccacct caaggcacat ctgatgcagt tccgcagtcg gtttttcgat ctgctcttca
    14821 atctggcgca gagtaaaacg gtaagcccga ctctcgtccc aatagatttc attgtcgatg
    14881 atatgaaagt cgaaaccgtt gtcagcggcg atttggtcca gatcccgtcg cacaggaacg
    14941 ttgtgtctca gcatgattag cctccccaat gcccacgggc gctggaagaa cgaccatagc
    15001 cgccgcgaga aacggttgac gcttttttgg tggtaaagcc gcctgaagag taagactctt
    15061 ttttgccaga accggagcgc caggagtaat caccagaagt gttgcgccag acggggcggg
    15121 aagcaaatga ggatccgccg ctgttataaa caaactgctc atcgcgatct ttacgaataa
    15181 cccggcttaa caaaaatcca gaaacgaccg ggatccaact ctgttcaacg ttgtcgtagt
    15241 agcaattttc gtacttagac tgacaattct gctgagtcat attcttcgga acatcggcat
    15301 aaaatgctgc tttggcgtta ttccagccac gcgcgcaaat gtctgcatta ttaccgtcat
    15361 caatacaatc ctgcactgtc gcgtaaaacg ttccgtcgcc gtcgttatcg acatcgccac
    15421 tatcgccaca acctttcaat acaaaaaaag cggcaccgcc cattagcgcc aacgttaagt
    15481 atttttgtgc gtagcgattg cgacgcggtt caaaaggatt attcggtcta ccaatgcgac
    15541 tgatcgctcc gtgaccaatt ttactgttat ttcttgattt gcgttttctg gccatgatcg
    15601 tccattacca tgtcatacaa gctgcgttaa aaataccgcc agccagcgcg gcggttccca
    15661 taaacattcc tgctgcggta ttgtgattaa taattttttc gcttaatgcg ggcatataaa
    15721 gtctgacacc agcaaaaacg agtaactgaa tcaccagtgc aatcccgccc caggcgaaat
    15781 agtctggaat actcaccgca ttgattgccg cactggataa ggggatcacg taacccaaca
    15841 atgtaccgct gaacgccagt gacgctgcgg tattattgtt tttgattaat tgccattcat
    15901 tgtgcggtgt aattttggag tagataaaca gaaaaataat caccatcacc acgccaataa
    15961 aaaaataggc actaaaggca agaagtgaat ccagtatatg cattgtaaac gtccttgtaa
    16021 ttaaccaata atatgaagtg atgtcagcgg gatatcgaca cccaacgcgc gcgaaaataa
    16081 ccactctggc tcgccgagat cgttaaaaga ttcctcacca tttagcagca aatattcgta
    16141 agtatcttcg gtcacttgcc gctggtagcc catggtaaaa ttatggactt cccatttagc
    16201 atgattttga ttttctactt tttccagcat gtagacgggt tcgatatttc ccggttcttc
    16261 gctgttaaaa aatctctgcc agcgtttttc ctgccagttt aaggtcattg cccccatcgt
    16321 tttggcgttg atcgcctcgc gccagtgact ctctttactg ataccgtagc tctcttcata
    16381 gacaaagagc ttgatatcat caatgtcatc aatatcttcg ccgccggtgg tattgatttg
    16441 cagaaactca tcgcctgaag tgtagtagcg gaaaatctga ctaccgccgc ccagatcgat
    16501 gtggctgaca gcggctaccg taaattcctc acccggcagc gcaatcagca attcatcttc
    16561 cagcaaacga aacgctaacg tgtcgagcgt aaagccacta ttgagatgaa gtcccagcgg
    16621 accgcgagcg atagcaggct tattatcctt gccaaacaga cgctggaaaa aaccagacat
    16681 attttatcct taaaatagtt gccagccttg tagtgaggct ggcaaaaaaa ttactcgccc
    16741 tgttggcgtt gcagtcttgc taatacatcc tgggcgctac ttttattgct accgccaatt
    16801 ccggcttccg ccagcttttc gtcaaggtcg cgaccgtctg cgactttctc caactgtgcg
    16861 gcagcatcca ggcgagcctg acgttctgcc tgacgcgttt gcaggcgttt taaggattct
    16921 gccgctgtcg aaacgctgga agatgcgcca acggtagagg ttgttacagc ctgttgtgca
    16981 cgctgcatgg cttcagtggc tttaacgact tccatttgct gctcaaactg agcgatacgc
    17041 tgcgctgtcg ctgtaactgc tttttccacg ccatcacggg agacttccag attcgacaac
    17101 acttgctctt cagcggtaat gagattctca aggcgcgcga tttcttcagc aacttcgtta
    17161 atcaacgacg gattaacgtt cttgcttaac gcttccagcg cacgggcttc cagactggct
    17221 ttgcgctcac gtaaatcttt cagcttatcg tgacttaatt tcacccgcgc cagcagatcg
    17281 acgcgagatt ttccggcttt atcgagttca gctttagcgt cacgaatatg ctgttccagc
    17341 atgcgcacgc cctgggtttc ttcaatggac tcttccgcct gggagataaa cgatttcccc
    17401 agcgtgaata aactttttaa aattcccata aatacccgtc cttgtaatta gtgtgaatat
    17461 tcttctgtaa tttcagccag atccaatgcg ttatctacca gcgaggctat ctcaagcagg
    17521 atatcttcaa gagaagattt aagcgataac gccccgaaaa caatgtaata ctcttcctgt
    17581 tgcacgctgg agattcctac cgatgacaac ggcatcatct tctgatttct taataagaag
    17641 gtattaaatt catctggatt gctgatacta cttaccggac aaataaaggt ttcgataatc
    17701 atttggcggg aagtaaaaag aatattgatt tgcaaatcgc cataatcatt cattttaata
    17761 attaatgcgt tatcgctatt tgttacatca atattttgtt caggtacagt ttgcagcgcc
    17821 gtcgccagcg ccaacggatt ccatgtcata tattattcca tatagattta gtttaaatat
    17881 taacaaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
    17941 tccaaatctg gatggctttt cataattcag aggaactagc tgcgctgacg caccgcttca
    18001 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
    18061 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
    18121 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
    18181 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
    18241 ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgtg
    18301 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcggcgtcc
    18361 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
    18421 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct
    18481 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
    18541 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
    18601 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
    18661 tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt
    18721 gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg
    18781 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
    18841 gcattttgtc agcaatctaa ccctcttctg tttgaagagg atattgatca ctctgccact
    18901 tttttcttcg ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt tttcgccgat
    18961 ggctttttcg cttttctcgc gtctttcttc gctgctttcg gcttcgtttt tttctcaccg
    19021 cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct
    19081 tttttgcctg catcgccttt tttcgctttc tcacgcgccg ttttaccgac gttgcgcggt
    19141 gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg
    19201 acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc acccatcagg
    19261 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccagc
    19321 ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt
    19381 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcc
    19441 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc
    19501 agcatctctt ccatcgaata atggtagcca ccggtttcag tggtgttgcc ctgatgcccc
    19561 tgctctttcg ccaacagata cttaatggcg cggtgcagcg tcaggtcagg ataacgacga
    19621 atcggcgaag taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttcc
    19681 ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca
    19741 ggacggtcgg caaccgactc cagcagctcc gcgtagtcgc gcggttccgg cttgttaccg
    19801 cccggcagtt ccagccccag ctctgccagc actgaacgga aagaggtaat cgcttcggtg
    19861 ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc
    19921 gccgagatat tcgccagaat catgcactct tcaattaact tgtgtgcgtc gttacgctga
    19981 gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc
    20041 tcaaatgaga tcccgccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc
    20101 aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc
    20161 agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
    20221 aatttgtagc cagtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
    20281 cggtctacct gcggattgag cgagcacaga ccgttagaga gcacttccgg cagcatcggg
    20341 ataacctgcg aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc
    20401 gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccacca
    20461 ccgcgttttt tctcgcagta aaccgcatcg tcaaagtcac gggcgtcttc gccatcaatg
    20521 gtaaccagcg gtaaatcgcg cagatcgaca cggcccgctt ttgcttcttc cggcacttct
    20581 tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
    20641 gtacgcagag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg
    20701 attttaccca ccgctttggt acggcgagtc gggcgttggg tcagttcgac tacgaccaca
    20761 aagcccatcc gcgcgcccat aatctgatcg ggcgggatta aaatatcgaa gctcagacgg
    20821 ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg
    20881 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagcg
    20941 cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
    21001 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
    21061 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg
    21121 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
    21181 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
    21241 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
    21301 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
    21361 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
    21421 caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt
    21481 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
    21541 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaagcgaa
    21601 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
    21661 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
    21721 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
    21781 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
    21841 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
    21901 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
    21961 agcccagcag gcaaaatatc tgccaggcgt accaaaatta cgcgtcgaac gggtcgcgca
    22021 gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccggtca
    22081 gctcttcaat acgtttgatg tagttcagcg ccgcctgcgg cagaccgcta cgttctttca
    22141 cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
    22201 agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata cggtaagcca
    22261 cacagagttt cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
    22321 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
    22381 gacgacgacc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
    22441 catcaaacag ttcagtcggg aacggacctg cacccacacg agtggagtaa gctttgagga
    22501 tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
    22561 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
    22621 cctgcgcacc ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
    22681 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
    22741 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
    22801 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
    22861 cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
    22921 ctttcgcgcc acgcgctttc tcacgcgcgt tatcaagcgc aacgtgataa tcaaggatca
    22981 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
    23041 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg
    23101 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
    23161 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
    23221 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
    23281 cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct
    23341 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
    23401 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
    23461 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
    23521 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
    23581 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atcccagagg
    23641 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
    23701 gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
    23761 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
    23821 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatacgg
    23881 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
    23941 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
    24001 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc
    24061 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
    24121 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgccgccggg
    24181 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
    24241 gtcagacgac cacgggaatc agtgacgata tctttcacgt ccaggcgacc aatttcagaa
    24301 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca
    24361 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg
    24421 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
    24481 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
    24541 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
    24601 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
    24661 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
    24721 cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
    24781 cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggca ccgttatcgc
    24841 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
    24901 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
    24961 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
    25021 caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
    25081 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
    25141 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
    25201 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca
    25261 tgtcatacgg gcggatagtc tcttccagtt cgcgctgagt atcgctacga atcacggtac
    25321 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aactccacgc agggcgctgt
    25381 cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat
    25441 tggtgacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
    25501 tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga
    25561 taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg
    25621 ttaccacgcc acgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
    25681 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
    25741 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttac
    25801 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
    25861 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
    25921 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
    25981 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
    26041 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
    26101 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
    26161 taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc
    26221 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
    26281 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
    26341 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
    26401 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca
    26461 atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc
    26521 accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg
    26581 acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
    26641 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
    26701 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
    26761 tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga
    26821 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt
    26881 tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca
    26941 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
    27001 gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
    27061 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
    27121 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
    27181 acctgcaatg cttcgacacc tgcggaagag accagagatt caaactcctg gaggtcttcc
    27241 atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
    27301 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
    27361 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
    27421 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcattgtt
    27481 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
    27541 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
    27601 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
    27661 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
    27721 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
    27781 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
    27841 cactatcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
    27901 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
    27961 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    28021 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    28081 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    28141 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    28201 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    28261 ctgccgcaac cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    28321 ccgcctgttg ctcaattctg gcccgtactt ccgggtctgc cgacggtagc ggcgacaacc
    28381 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    28441 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    28501 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    28561 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    28621 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    28681 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    28741 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    28801 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    28861 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    28921 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    28981 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    29041 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    29101 tttagccgca acggaatcag caacggctgg gcgcaaacgg gcgcttcacc cggcgtcagt
    29161 tgtacctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg
    29221 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    29281 ttcgccgcaa gtgcaggttc atgcggttct ggcgcttttg gtttttgcat cggcgcgggc
    29341 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtaacctgg ttgcgcattc
    29401 ggccaggggg cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctact
    29461 ggctcacgaa ctgccggttc agcaaagtga ttgcgccccg ccgccacgcg gttttccggg
    29521 atggcacgcg gtgtgggttg tggttcatcg tccagcggta gcggcgtttc cagctgctgt
    29581 tgtagcacgc tcagcacgcc ctgatagata aagtcatgca ccaggcgcga ctgatggaaa
    29641 cgcacttcgt gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga
    29701 tacaacacaa acgccggttg ttgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    29761 gcgtgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc
    29821 gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgcagcgt gagatcgccg
    29881 tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccacaaat cgcgcctaag
    29941 cgccgttctt tttgcccgcc ttccggcact gcgcggtact ggcgtacgat tttgccgtta
    30001 tgcgacaggt tgatcgtgac gtcgaaacgc gccagtgcga tgcggcggat gatttcatcg
    30061 atatggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    30121 aacaggtcca gtacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg
    30181 tccatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    30241 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    30301 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc
    30361 gccagcgcca gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    30421 gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc
    30481 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    30541 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtacga
    30601 tctggcgtcg tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc
    30661 ggatgcgcaa ggaaataatt gcgcagacct ttataaatgg cttctgccag ctgttgttgg
    30721 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    30781 agtactgatg ggatatccgg cgaacgcaga acgccaaggc tggcgtgttc tggtcgacgt
    30841 ttatgaattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    30901 acccgctggg aatgaccgaa ctgtaaatcc agcacagcct ggcttaaata ggggtcagac
    30961 tgactgttcg ccagcacatc gcccgctccg cccagtagct ccgactgttt ctcgtgctgc
    31021 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    31081 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    31141 tgcttacgtg ccacatcgct gcgccccatc accgagataa agtaatcccc gtcacgggtt
    31201 aaaacgccct taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    31261 atggtgacat ttttctcccg cgtaccgccg gggccgatag cgccagggtc ctgaccgccg
    31321 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    31381 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    31441 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg taggcgtttc aacgcgtttc
    31501 gcaaccacag gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt aaagacgaca
    31561 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg
    31621 accaccagcc gtagcgtttg tgcatcttta ggcgttccgg agcgaatcgc cttcaccaga
    31681 ttattaccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    31741 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    31801 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    31861 acctgcgcgc acagcagcag caacgtcgct accaaccaat ttctgatgcg atacatcatc
    31921 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    31981 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    32041 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    32101 gcaaaatagt cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    32161 tcaaagtgat aaaccattaa gttgtcgagc gtatagggtt cgaccagcgt ataggtcggg
    32221 cttttgacat taccctgatg acccaaagcc tgcaaaaagc cccggctaaa ggtggtttta
    32281 cctgcgccta aatcgccata cagatagatg acggttgcac catcgcaggc tttggctacc
    32341 cgctcgccca ggtctaaggt tgcctgctca tcagggagcg gaattactcg attcatcatg
    32401 gtttttttca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    32461 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac
    32521 acagcctgca caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    32581 accagagagc acatcgccca tgccgccgct tgccatgcct gcatttccgg catcaataat
    32641 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    32701 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    32761 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    32821 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    32881 aaaattctca actttttgca gggctttttt cccccactct tgctggccca gaccgggacc
    32941 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg
    33001 caccatcaat tctggtcgtg cagtcagcag cgacgcaatg ttctcactgc gggtcagtac
    33061 tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa tagcccccgc
    33121 cgtgccgtga tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg
    33181 gcgacgcggt ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
    33241 ctgacctgcc agccaactat ccaaccccag tgagtcaaaa tgcagttgtc cggtaacatc
    33301 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
    33361 gttgatcacc gcacctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac
    33421 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    33481 gggcgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    33541 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
    33601 cgcggcctct tccggcaacg gtttgtcgct atcctgggcc aacaacgtga cctcaatgcc
    33661 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    33721 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    33781 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
    33841 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
    33901 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    33961 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    34021 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    34081 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt
    34141 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    34201 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    34261 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    34321 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    34381 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    34441 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa
    34501 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    34561 tgctcgccgc tgtatctctt atctcaccat cgaactggaa ggggcgatcc cggaagagtt
    34621 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    34681 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
    34741 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    34801 atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
    34861 caatgcgccc tgggatgaaa cgattctggc ggcgctggag agtcgcaaag gtgagcaccc
    34921 acttcttgat gagcacatag cgtgggcgat tgcgcagcaa atcgagagac gaaatgcgtg
    34981 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtaattg aaaaagggtt
    35041 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    35101 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta
    35161 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    35221 cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc
    35281 aataacgcat cgcgtcgctg tggatatttt attgagagaa gaatttggag cgggaa
//
LOCUS       DAZGCQ010000049        34984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000049 DAZGCQ010000000
VERSION     DAZGCQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.049"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..959
                     /locus_tag="ACHK07_002557"
     CDS             <1..959
                     /locus_tag="ACHK07_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO8102873.1"
                     /translation="NEFIINLKELTRKYNFSHGDLENILLAKDYLGVSWIPKALRNNQ
                     NKIVKAWLLAIDDFEKEFEVNKNEMLLSIVNEIDSTYDLNSAIKTNDYAVVNILLANI
                     KAKMFKNEINKEDILKLMAAREKVAGASDKWTKASGLYSAIVKGHTEIVAAWMETAEV
                     IASHYENDKDVVRELLSLSRNNAVCSLYVASYKTMSKQVIDVYLNAAIRLALQHGFTF
                     DEIVEQFTRDFDGKSFSHVVEKADDIYMGLWLKIFKIVVGENENYLKDVMMQLEAKNN
                     EGKSVISQANGNPVFKELFWKAIDEFNFPQEELNRLNQYRSL"
     gene            complement(1098..2462)
                     /gene="mnmE"
                     /locus_tag="ACHK07_002558"
     CDS             complement(1098..2462)
                     /gene="mnmE"
                     /locus_tag="ACHK07_002558"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO8102874.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2568..4214)
                     /gene="yidC"
                     /locus_tag="ACHK07_002559"
     CDS             complement(2568..4214)
                     /gene="yidC"
                     /locus_tag="ACHK07_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO8102875.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(4217..4474)
                     /gene="yidD"
                     /locus_tag="ACHK07_002560"
     CDS             complement(4217..4474)
                     /gene="yidD"
                     /locus_tag="ACHK07_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO8102876.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4438..4797)
                     /gene="rnpA"
                     /locus_tag="ACHK07_002561"
     CDS             complement(4438..4797)
                     /gene="rnpA"
                     /locus_tag="ACHK07_002561"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO8102877.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4814..4954)
                     /gene="rpmH"
                     /locus_tag="ACHK07_002562"
     CDS             complement(4814..4954)
                     /gene="rpmH"
                     /locus_tag="ACHK07_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO8102878.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(5184..5264)
                     /locus_tag="ACHK07_002563"
     CDS             complement(5184..5264)
                     /locus_tag="ACHK07_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102879.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5561..6964
                     /gene="dnaA"
                     /locus_tag="ACHK07_002564"
     CDS             5561..6964
                     /gene="dnaA"
                     /locus_tag="ACHK07_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO8102880.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6969..8069
                     /gene="dnaN"
                     /locus_tag="ACHK07_002565"
     CDS             6969..8069
                     /gene="dnaN"
                     /locus_tag="ACHK07_002565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO8102881.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            8069..9142
                     /gene="recF"
                     /locus_tag="ACHK07_002566"
     CDS             8069..9142
                     /gene="recF"
                     /locus_tag="ACHK07_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO8102882.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            9171..11585
                     /gene="gyrB"
                     /locus_tag="ACHK07_002567"
     CDS             9171..11585
                     /gene="gyrB"
                     /locus_tag="ACHK07_002567"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO8102883.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11609..11707)
                     /locus_tag="ACHK07_002568"
                     /pseudo
     CDS             complement(11609..11707)
                     /locus_tag="ACHK07_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11825..12223
                     /gene="yidB"
                     /locus_tag="ACHK07_002569"
     CDS             11825..12223
                     /gene="yidB"
                     /locus_tag="ACHK07_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO8102884.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGDVAPQANNDLLSAGMELLKGKLFR"
     gene            12338..13150
                     /gene="yidA"
                     /locus_tag="ACHK07_002570"
     CDS             12338..13150
                     /gene="yidA"
                     /locus_tag="ACHK07_002570"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO8102885.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(13196..13852)
                     /gene="yidX"
                     /locus_tag="ACHK07_002571"
     CDS             complement(13196..13852)
                     /gene="yidX"
                     /locus_tag="ACHK07_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO8102886.1"
                     /translation="MKLNFKGFFKAACLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13988..14101
                     /locus_tag="ACHK07_002572"
                     /pseudo
     CDS             13988..14101
                     /locus_tag="ACHK07_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14098..15162)
                     /gene="cbrA"
                     /locus_tag="ACHK07_002573"
     CDS             complement(14098..15162)
                     /gene="cbrA"
                     /locus_tag="ACHK07_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO8102887.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDNKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFICGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            15263..16477
                     /gene="yidR"
                     /locus_tag="ACHK07_002574"
     CDS             15263..16477
                     /gene="yidR"
                     /locus_tag="ACHK07_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO8102888.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAESGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16479..16811)
                     /gene="yidQ"
                     /locus_tag="ACHK07_002575"
     CDS             complement(16479..16811)
                     /gene="yidQ"
                     /locus_tag="ACHK07_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8102889.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASASM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    17051..17123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            17117..17530
                     /gene="ibpA"
                     /locus_tag="ACHK07_002576"
     CDS             17117..17530
                     /gene="ibpA"
                     /locus_tag="ACHK07_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO8102890.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17642..18070
                     /gene="ibpB"
                     /locus_tag="ACHK07_002577"
     CDS             17642..18070
                     /gene="ibpB"
                     /locus_tag="ACHK07_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO8102891.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            18266..19927
                     /gene="yidE"
                     /locus_tag="ACHK07_002578"
     CDS             18266..19927
                     /gene="yidE"
                     /locus_tag="ACHK07_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO8102892.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19924..20640)
                     /gene="yidP"
                     /locus_tag="ACHK07_002579"
     CDS             complement(19924..20640)
                     /gene="yidP"
                     /locus_tag="ACHK07_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8102893.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20936..22552
                     /locus_tag="ACHK07_002580"
     CDS             20936..22552
                     /locus_tag="ACHK07_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8102894.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22552..23874
                     /locus_tag="ACHK07_002581"
     CDS             22552..23874
                     /locus_tag="ACHK07_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO8102895.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23871..24764)
                     /gene="yidL"
                     /locus_tag="ACHK07_002582"
     CDS             complement(23871..24764)
                     /gene="yidL"
                     /locus_tag="ACHK07_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8102896.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24931..26646
                     /gene="yidK"
                     /locus_tag="ACHK07_002583"
     CDS             24931..26646
                     /gene="yidK"
                     /locus_tag="ACHK07_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO8102897.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26643..28136
                     /gene="yidJ"
                     /locus_tag="ACHK07_002584"
     CDS             26643..28136
                     /gene="yidJ"
                     /locus_tag="ACHK07_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8102898.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEDKKQKF"
     gene            complement(28183..28632)
                     /gene="yidI"
                     /locus_tag="ACHK07_002585"
     CDS             complement(28183..28632)
                     /gene="yidI"
                     /locus_tag="ACHK07_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO8102899.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28741..29088
                     /gene="yidH"
                     /locus_tag="ACHK07_002586"
     CDS             28741..29088
                     /gene="yidH"
                     /locus_tag="ACHK07_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO8102900.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            29078..29440
                     /gene="yidG"
                     /locus_tag="ACHK07_002587"
     CDS             29078..29440
                     /gene="yidG"
                     /locus_tag="ACHK07_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO8102901.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29437..29934
                     /gene="yidF"
                     /locus_tag="ACHK07_002588"
     CDS             29437..29934
                     /gene="yidF"
                     /locus_tag="ACHK07_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8102902.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29942..31126)
                     /gene="emrD"
                     /locus_tag="ACHK07_002589"
     CDS             complement(29942..31126)
                     /gene="emrD"
                     /locus_tag="ACHK07_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO8102903.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            31208..31282
                     /locus_tag="ACHK07_002590"
     CDS             31208..31282
                     /locus_tag="ACHK07_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102904.1"
                     /translation="MNVSQICARNGEKKNGRICKQKRQ"
     gene            complement(31406..31495)
                     /gene="tisB"
                     /locus_tag="ACHK07_002591"
     CDS             complement(31406..31495)
                     /gene="tisB"
                     /locus_tag="ACHK07_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO8102905.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            32060..32158
                     /gene="ivbL"
                     /locus_tag="ACHK07_002592"
     CDS             32060..32158
                     /gene="ivbL"
                     /locus_tag="ACHK07_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO8102906.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            32264..33952
                     /gene="ilvB"
                     /locus_tag="ACHK07_002593"
     CDS             32264..33952
                     /gene="ilvB"
                     /locus_tag="ACHK07_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO8102907.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33956..34246
                     /gene="ilvN"
                     /locus_tag="ACHK07_002594"
     CDS             33956..34246
                     /gene="ilvN"
                     /locus_tag="ACHK07_002594"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8102908.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34509..>34984
                     /locus_tag="ACHK07_002595"
     CDS             34509..>34984
                     /locus_tag="ACHK07_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102909.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINVAAELDITNSEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVL"
BASE COUNT         8512 a         8798 c         9220 g         8454 t
ORIGIN      
        1 ttaatgaatt cataataaat ttaaaagagt tgaccagaaa gtataatttt agccatggcg
       61 atctggaaaa tatactcctt gcgaaagatt acctcggtgt gagctggatt cctaaggctt
      121 taagaaataa tcaaaataaa attgtcaaag catggttgtt ggcgatagat gactttgaga
      181 aagaatttga ggtaaataaa aatgaaatgc ttcttagtat agtaaatgaa atagactcaa
      241 cttatgattt aaatagcgct attaaaacca atgattatgc tgttgttaat atattattag
      301 ccaatataaa agccaaaatg tttaaaaatg aaataaataa agaagatata ttgaaactga
      361 tggcagcaag agaaaaagtg gcgggagcat cagacaaatg gacgaaggca tcaggcttat
      421 attctgcgat agtgaaaggg catacggaga ttgttgctgc ctggatggag acagctgaag
      481 tgatagccag ccattatgaa aatgataaag atgtagtgag agaactactg tcgctgagca
      541 gaaataatgc agtttgctct ttgtatgttg ccagctataa gacaatgagt aagcaggtca
      601 ttgatgtata tctgaatgcg gcgattcgcc tggcgttgca acacgggttc actttcgatg
      661 aaattgtgga gcagtttacc cgtgactttg atgggaagtc attctctcat gtggtagaga
      721 aagcggatga tatatatatg ggactctggc tgaaaatatt caaaattgtg gttggtgaaa
      781 atgaaaatta tcttaaagat gtgatgatgc aactggaagc gaagaataat gaaggtaaat
      841 ctgtaataag tcaggcaaat ggcaatcctg tatttaaaga actattctgg aaagcaatag
      901 acgaatttaa ctttccgcaa gaggagctta atcggttaaa ccaatatcgt tctctgtaaa
      961 gacaggactg caagatgttg aaagtcgctc cagccagtag taaaggcagt tttggtgcag
     1021 gatgctcata aaaaaacaag cgctgttccg ttctgatagt agaacggaac agtgcccttt
     1081 atatcaatta agacgactta cttaccaata cagaagctgg agaaaatccg ccccagcagg
     1141 tcgtcagagg taaattcccc ggtgatttcg cttaagttct gctgcgccag gcgcaactct
     1201 tccgccagta gttcgcctgc ccaggcacct aacagttgcg ctttaccttg ctgcaaatgc
     1261 tccgctgcct gttccagcgc ctgtaggtgg cgacggcgcg ccaggaagcc gccttccatg
     1321 ttggtgtcaa agcccatgct ctgtttgaga tggttacgca gcacgtccac gccttcgcca
     1381 gtccttgcag acagacgaat taacgcgtga ccgttcactt cacttattcc cagcgtttcg
     1441 ccggtgatat cggctttatt gcgcaccacg gtgatcggca gtttcgctgg cagacgggcg
     1501 ataaattccg gccagatctc tgccggatca acggcgtctg tcgtggtgcc atcgaccata
     1561 aacagcacgc ggtcggcctg ttcaatttcc tgccacgcgc gctcgatccc aatacgttca
     1621 acttcgtcac tggcttcacg tagcccggcg gtatcgatga tatgcagcgg cattccgtca
     1681 atatggatat gctcacgcag cacgtcacgc gtggttccgg cgatatcggt tacgattgcc
     1741 gcttcacggc ccgccagcgc gtttaacagg ctcgatttac cggcgttagg acgtccggca
     1801 atcaccacct tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca
     1861 tcgagatcgg caatcacgtc attaagctgg gcttcaattt ttccgtcgga gaggaaatcg
     1921 atctcttcat cggggaaatc aattgccgct tcgacgtaaa tgcgcaagtg ggtgagggct
     1981 tccaccagat ggttgacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg
     2041 gctgcctgtt ccgaactggc atcgataaga tcggcaatcg cctcggcctg ggctaagtcg
     2101 agtttatcgt taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg
     2161 ggaatggtca gaatgcgttt taacagcagg tcgaggatca ccggaccgcc atgaccctgc
     2221 agttccagca catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc
     2281 tgatcgagca cgctgccgtc ggcgtcttta aacggaagat aatcggcgta gcgcggctta
     2341 ggcagtttac ccagcacggt ttcggcaact tcacgggctt tgaagccgga gatgcgcagg
     2401 atgccaacgc cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc
     2461 atgatgttcc tgttgctttg tgtggcggat gcgcggtgct tatccgccct acgaaaagaa
     2521 aaaaggcggt caactgaccg cccttatttt agcgaaaact caccgaatca ggattttttc
     2581 ttctcgcggc tatgcaggcc acgtttttcc agaccacggt aaatcagctg ctgctgaata
     2641 atggttacca ggttgctgac gatatagtac agcaccagac ctgacgggaa ccacaggaag
     2701 aacacggtga agatgaccgg cataaaggtc atgatcttct gctgcatcgg gtcggtcaca
     2761 gtggtcggcg acatcttctg aatgaagaac atcgttacgc ccatcaggat cggcaggatg
     2821 tagtacgggt cctgtgcaga caggtcgtgg atccacagtg cgaacggcgc ctgacgcagt
     2881 tcaacggaac ccatcagcat gtagtacaac gccaggaaga ttggcatctg gatcagcagc
     2941 gggaagcagc cgcccagcgg gttaaccttc tcagctttgt acagcgccat catttcctgg
     3001 ctgatacgct gtttgtcatc gcccagacgc tcacgcatcg cctgaatctt cggctgcagc
     3061 atacgcatct tcgccatgga ggtgtactgc gctttggtca gcgggtacat gatgccacga
     3121 acgataaagg tgatgatgat aatggagaag ccccagttac ccacaaagct atggatccat
     3181 ttcagcagtt tgaacagcgg ctgagagatg aaccacaacc aaccgtaatc aacggtcaga
     3241 tccaggtgcg gagcaacagc tgccattttg tcctggattt ccgggccaac ccacagggtg
     3301 ctgttcatcg cgccagtctg accaggctga accagtaccg gctgagattt atagccgata
     3361 gcggcgatgc cgttacccag attagcagta tagaagttgt tggtaccgtc gttatgcggg
     3421 atccacgccg tcgcgaaata ctgttgcagc attgccaccc aaccaccttt cgaagagatg
     3481 ttcaggtttt cgttatcggc aatggtatcg aacttgtatt tctcatactt ctcgtcaggc
     3541 gtggagtacg ccgcgccacg gaaggtgtgc agtgcgaagt tgctgcttcc ggtatcgaga
     3601 tgcggtggca gagtgatgga ttgcttcaac tgaccaaatg aggagatttc cagcggtttc
     3661 tcgccagcat tctgcacgtt gtagttgacg ttgacagcgt aatcaccacg ttttaggaca
     3721 aacgttttgg taaacgtgtt gcctgccgcg tcggtatacg tcatcggcac ctgcagttcg
     3781 ttttgacctt cagccagcac ataagcgtct ttttcaacgt tatacagcgg acgcgggccg
     3841 ttagccgggt tatccgggcc atcacgaccg gtcagaccgc tctgtgcctg ataaataaac
     3901 tgcggtgaag tttccagcag ctggaacggc tgggtagagt tcagctcttt cgggtaagca
     3961 ggcagcagag cttgctcaac atcaccacca cgggtgttga tggtcagatc aagcacgtcg
     4021 gtcttaaccg agatcagttt cccctggcca ctggccggta cgccctggtc ggcggcgcta
     4081 cccgctgcgg tggtcgttgt ctgcgtggtc tgttgggcct gaggttgcgg gtttttatcc
     4141 tgctcccagg cttgccagat catgaaagac acgaacagca aagcgatgac taaaagattg
     4201 cgttgcgaat ccatcgttag tgttctctgg tatcaaatgg tccgggcggg acgggatcgt
     4261 caccaccagg gtgtaaaggg tggcatttta atacgcgttt caccgtcaac caactgcctt
     4321 ttatcactcc aaacctgcgc aatgcctcaa ttccgtagct tgaacaggtt ggagtgaaac
     4381 gacaatgcgg cccgagtagc ggactaatca ggcgttgata gacccgaatg agggctatca
     4441 ggacccgcga gccaggcgac agtggcggcg ccataatttt tccaacgctt ccgagagagc
     4501 acggttatcg aggtcggcaa cccctttttt cgccaccacc acgaaatcca tagccgggag
     4561 ttcatgttgg cgcagacgga agctttcacg cgtcagacgt ttaatccgat tgcgttcatg
     4621 ggcgcgtcga acgtttttct tggcgactgt aagaccgata cggggatgcc ccagcgaatt
     4681 caggcggccg agaatggtaa tttgcggcgt gccagcccgt tgtggctgct ggaagacgaa
     4741 tgtgaattga ctgggagtta acaagcgtaa ctccctggga aatgcgagct taaccactca
     4801 ggggttagct ttattactta gaaacggtca gacgagcgcg gcctttagca cgacgacgtg
     4861 ccagaacctg acgaccattt ttagtagcca tacgagcacg gaagccgtga gaacggttgc
     4921 gcttcagtac agacggttga aaagtgcgtt tcatggcgat ttctacctaa acttgaataa
     4981 attcaatggc tttattggat atccgccgaa aaatgaaacg atggacaccg aagccatggg
     5041 tgattaaaga ggccggattg taataattgt acactccgga gtcaattctc tttccttatt
     5101 taccgcgctt ttccgcacct tttcgcaggg aaaacgtacg gcctcacacc agtgaaaacc
     5161 agcatggcgc gccgggtgga ggattatacg ggctgacggg taaagcgcaa ggatcgtcct
     5221 ggatctttat tagatcgatt aagccaattt ttgtctatgg tcattaaatt ttccaatatg
     5281 cggcgtaaat cgtgcccgcc tcgcggcagg atcgtttaca cttagcgagt tctggatcat
     5341 cctgtggata aatcgggaaa atctgtgaga aacagaagat ctcttgcgca gtttaggcta
     5401 tgatccgcgg tcccgatcgt tttgcaggat cttgatcggg catataaccg cagacagcgg
     5461 ttcgtgcgtc accctcaagc agggtctttt cgacgtacgt caacaatcat gaatgtttca
     5521 gccttagtca ttatcgactt ttgttcgagt ggagtccgcc gtgtcacttt cgctttggca
     5581 gcagtgtctt gcccgattgc aggatgagtt accagccaca gaattcagta tgtggatacg
     5641 cccattgcag gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt
     5701 cctcgattgg gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg
     5761 cggagcggat gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc
     5821 acaagcggca gtgacgagca acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca
     5881 acgtgctgcg ccttctacgc gctcaggttg ggataacgtc ccggccccgg cagaaccgac
     5941 ctatcgttct aacgtaaacg tcaaacacac gtttgataac ttcgttgaag gtaaatctaa
     6001 ccaactggcg cgcgcggcgg ctcgccaggt ggcagataac cctggcggtg cctataaccc
     6061 gttgttcctt tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa
     6121 cggcattatg gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt
     6181 tcaggacatg gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg
     6241 ttccgtagat gcactgctga tcgacgatat tcagtttttt gctaataaag aacgatctca
     6301 ggaagagttt ttccacacct tcaacgccct gctggaaggt aatcaacaga tcattctcac
     6361 ctcggatcgc tatccgaaag agatcaacgg cgttgaggat cgtttgaaat cccgcttcgg
     6421 ctgggggctg actgtggcga tcgaaccgcc agagttagaa acccgcgtgg cgatcctgat
     6481 gaaaaaggcc gacgaaaatg acattcgttt gccaggcgaa gtggcgttct ttatcgccaa
     6541 gcgtctacga tctaacgtac gtgagctgga aggcgcgctg aaccgcgtca ttgccaacgc
     6601 caactttacc ggacgggcga tcactatcga cttcgtgcgt gaggcgctgc gcgacttgct
     6661 ggcattgcag gaaaaactgg tcaccatcga caatattcag aagacggtgg cggagtacta
     6721 caagatcaaa gtcgcggatc tcctttccaa gcgtcgatcc cgctcggtgg ctcgtccgcg
     6781 tcagatggcg atggcgctgg cgaaagagct gactaaccac agtctgccag agattggcga
     6841 tgcgtttggt ggtcgtgacc acactacggt gcttcatgcc tgccggaaga tcgagcagct
     6901 gcgtgaagag agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc
     6961 gtaaacctat gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga
     7021 gcggtccgtt aggtggtcgt cctacgctac cgattctcgg taatctgctg ttacaggttg
     7081 ctgacggtac gttgtcgctg accggtactg atctcgagat ggaaatggtg gcacgcgttg
     7141 cgctggttca gccacacgag ccaggagcga cgaccgttcc ggcgcgcaaa ttctttgata
     7201 tctgccgtgg tctgcctgaa ggcgcggaaa ttgccgtgca gctggaaggt gagcggatgc
     7261 tggtgcgctc cgggcgtagc cgtttttcgc tgtctaccct gccagcggcg gatttcccga
     7321 acctcgatga ctggcagagt gaagtcgaat ttaccctgcc gcaggcgacg atgaagcgtc
     7381 tgattgaagc gacccagttt tctatggcgc atcaggacgt tcgctattac ttaaacggta
     7441 tgctgtttga aaccgaaggt gaagaactgc gcaccgtggc gaccgacggc caccgtctgg
     7501 cggtctgttc aatgccaatt ggtcaatctt tgccaagcca ttcggtgatc gtgccgcgta
     7561 aaggcgtgat tgaactgatg cgtatgctcg acggcggcga taatccgctg cgtgtgcaga
     7621 ttggcagcaa caatattcgc gcccacgttg gcgactttat cttcacctct aaactggtgg
     7681 atggtcgctt cccggattac cgccgcgttc tgccgaagaa tccggacaaa catctggaag
     7741 caggctgcga tctcctcaag caggcgtttg ctcgcgcggc aattctctct aacgagaaat
     7801 tccgcggcgt gcgtctgtat gtcagcgaaa accagctgaa aatcaccgcc aataacccgg
     7861 aacaggaaga agcggaagag atcctcgacg ttacctatag cggtgcggag atggaaatcg
     7921 gcttcaacgt cagctatgtg ctggatgtcc tgaacgcgct gaaatgtgaa aacgtccgca
     7981 tgatgctgac cgattcggtt tccagtgtgc agattgaaga tgccgcatca cagtcggctg
     8041 cctatgttgt catgccaatg agactgtaat gtccctcacc cgcttgttga tccgcgattt
     8101 ccgcaacatt gaaaccgcgg atctcgcctt atctcccggc tttaactttc tggtaggtgc
     8161 caacggcagt ggcaaaacca gcgtgctgga agccatctat acgctcggcc atggtcgggc
     8221 gtttcgcagt ttgcagattg gtcgcgtcat tcgccatgag caggaggcgt ttgttctcca
     8281 cgggcgatta cagggcgaag agcgcgagac ggcgattggc ttaaccaaag acaaacaggg
     8341 cgacagcaaa gtccgcatcg acggtactga cgggcataaa gtcgcggaac tggcgcacct
     8401 gatgccaatg cagctgataa cgccagaagg gtttacttta ctcaacggcg gccccaaata
     8461 cagaagagca ttcctcgact ggggatgctt tcacaacgaa cccggatttt tcaccgcctg
     8521 gagcaatctc aagcgattgc tcaagcagcg caatgcggcg ctgcgccagg tgacacgtta
     8581 cgaacagcta cgcccgtggg ataaagaact gatcccgctg gcagagcaaa tcagcacctg
     8641 gcgcgcggag tatagcgccg gtatcgcggc cgatatggct gatacctgta agcaatttct
     8701 ccctgagttt tctctgactt tctctttcca gcgcggctgg gagaaagaga cagaatatgc
     8761 tgaggtgctg gaacgtaatt ttgaacgcga tcgccagcta acctacaccg cgcatggccc
     8821 gcataaagcg gacttacgca ttcgcgccga cggtgcgccg gtggaagata ccttatcgcg
     8881 tgggcagctt aagctgttga tgtgcgcctt acgtctggcg caaggagagt tcctcacccg
     8941 tgaaagcggg cggcggtgtc tctacctgat agatgatttt gcctctgagc ttgatgatga
     9001 gcgtcgcggg ctgcttgcca gccgcttaaa agcgacgcaa tcgcaggtct ttgtcagcgc
     9061 gatcagtgct gaacacgtta tagacatgtc ggacgaaaat tcgaagatgt ttaccgtgga
     9121 aaagggtaaa ataacggatt aacccaagta taaatgagcg agaaacgttg atgtcgaatt
     9181 cttatgactc ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg
     9241 gtatgtatat cggcgacacg gatgacggca ccggtctgca ccacatggta ttcgaggtgg
     9301 tagataacgc tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc
     9361 acgccgataa ctctgtctcc gtacaggatg atgggcgcgg cattccgacc ggtattcacc
     9421 cggaagaggg cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat
     9481 ttgacgataa ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa
     9541 acgccctgtc gcaaaaactg gagctggtta ttcagcgcga gggtaaaatt caccgtcaga
     9601 tctacgaaca cggtgtaccg caggctccgc tggcggttac cggcgagact gaaaaaaccg
     9661 gcaccatggt gcgtttctgg cccagcctcg aaaccttcac caatgtgacc gagttcgaat
     9721 atgaaattct ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc
     9781 gtctgcgcga caagcgtgac ggcaaagaag accacttcca ctatgaaggc ggcatcaagg
     9841 cgttcgttga atatctgaac aagaacaaaa cgccgatcca cccgaatatc ttttacttct
     9901 ccaccgaaaa agacggtatt ggcgtcgaag tggcgttgca gtggaacgat ggcttccagg
     9961 aaaacatcta ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag
    10021 gcttccgtgc ggcgatgacc cgtaccctga atgcctacat ggacaaagaa ggctacagca
    10081 aaaaagccaa agtcagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt
    10141 ccgtaaaagt gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg
    10201 aggtgaaatc ggcggttgaa cagcagatga acgaactgct ggcggaatac ctgctggaaa
    10261 acccaactga tgcaaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag
    10321 ctgcgcgtcg cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc
    10381 cgggcaaact ggcagactgc caggagcgcg atccggcgct ttccgaactg taccttgtgg
    10441 aaggggactc cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgatcc
    10501 tgccgctgaa aggtaaaatc cttaacgttg agaaggcgcg cttcgacaag atgctctctt
    10561 ctcaggaagt ggcgacgctt atcaccgcgc ttggttgtgg tatcggtcgt gacgagtaca
    10621 acccggacaa actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct
    10681 cgcacattcg tacgctgctg ttgaccttct tctatcgtca gatgccggag atcgttgaac
    10741 gcggtcacgt ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac
    10801 agtacattaa agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggacggcg
    10861 caacgctgca caccaacgcc agcgcaccgg cattggctgg cgaagcgtta gagaaactgg
    10921 tgtctgagta caacgcgacg cagaaaatga tcaaccgcat ggagcgtcgt tatccgaaag
    10981 caatgctgaa agagcttatc tatcagccga cgctgacgga agccgacctc tctgatgagc
    11041 agaccgttac ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg
    11101 gcagccagtg gaagtttgat gtccacacca atgccgaaca aaacctgttt gaaccgattg
    11161 ttcgcgtgcg tacccacggt gtggatactg actatccgct ggatcacgag tttatcaccg
    11221 gtggcgaata tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag
    11281 atgcgtttat cgaacgtggc gagcgtcgtc agccggtagc cagctttgag caggcgctgg
    11341 actggctggt gaaagagtct cgtcgcggtc tctccatcca gcgttataaa ggtctgggcg
    11401 agatgaaccc ggaacagctg tgggaaacca ccatggaccc ggaaagccgt cgtatgctgc
    11461 gcgttaccgt taaagatgcg attgctgccg accagttgtt caccacgctg atgggcgacg
    11521 ccgttgaacc gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatattgata
    11581 tttaatggcg ttaaccatgc gagcgtgcct gatgcgctac gcttatcaag cctacgagac
    11641 tcctgcaata tattgatttt gcatgttttt gcaggccgga taaggcgttc acgccgcatc
    11701 cggcaagaag ctataagaaa agggcggaga tcatctccgc cctttttatt tctgcaatcc
    11761 gcaattatca tcccgcagtt ctatactttt tgggtcaaca cgtaacgctt tacaggtgat
    11821 tatcatgggg ctttttgatg aagttgtcgg cgcctttctg aaaggcgatg cggggaaata
    11881 tcaggcgatt ttaagttggg ttgaggagca gggcggcatt caggtgctgc tggaaaaact
    11941 gcaaagtggc ggcttagggg ccattctctc aacctggctg agtaatcaac agggcaatca
    12001 atcggtgagc ggcgaacaac tggaatcggc gctcggcaca aatgcggtat ccgatctcgg
    12061 gcaaaaactt ggcgtggata ccagtacggc ttccagttta ctggcagaac aattgccgaa
    12121 gattattgat gctctctcgc cacagggcga tgtagcgccg caagccaaca atgatttact
    12181 ttccgcaggg atggaactac tgaaagggaa actcttccgc taagcaaatg gggagcatgc
    12241 aacccgcgtt gcatccccct tttccctcgg gtgttgtact gatttttgag cggaatcgcg
    12301 ttagcatggg tcaggaacca atctacctgg ggaactcatg gctatcaaac tcattgctat
    12361 cgatatggat ggcacccttc tgctgcccga tcacaccatt tcacctgccg ttaaaaatgc
    12421 gattgccgca gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc gcccttatgc
    12481 aggtgttcac aattacctga aagagctgca tatggaacag ccgggtgact actgcattac
    12541 ttataacggc gcgctggtac agaaggccgc tgatggtagc accgtggcgc aaactgctct
    12601 cagctatgac gactaccgtt tcctggaaaa actctctcgc gaagtcggtt ctcatttcca
    12661 cgcactggac cgcaccacgc tgtacaccgc caaccgtgat atcagctact acacggtgca
    12721 tgaatccttt gttgccacta ttccgctggt gttctgcgaa gcggagaaaa tggaccccaa
    12781 tacccagttc ctgaaagtga tgatgattga tgaacctgcc atcctcgacc aggctatcgc
    12841 gcgtattccg caggaagtga aagagaaata taccgtgctg aaaagtgcgc cgtacttcct
    12901 cgaaatcctc gataaacgcg ttaacaaagg tacgggtgtg aaatcactgg ccgacgtgtt
    12961 aggtattaaa ccggaagaaa tcatggcgat tggcgatcag gaaaacgaca ttgcaatgat
    13021 tgaatatgca ggcgtcggtg tggcgatgga taacgctatt ccttcggtga aagaagtggc
    13081 gaactttgtc accaaatcta accttgaaga tggcgtggcg tttgctattg agaagtatgt
    13141 gctgaattaa tcagtggacg ggcaaacagc ataaacgcgt ttgcccgctt actgattatt
    13201 ggtttactgc atatacaacc agaaattgca gcggtgcagt aatgatgtcc agcgtaacag
    13261 tcactggcag taatgctgcg gaaacaccat ccggaatgcc tctgggtgaa tagtattcat
    13321 aaaatcgcac tttaaatgga tggtagaacg ccatcacctt tgagtaatcc atgtttttat
    13381 ttttctcatg gattgtgcct tttaggtttt ccagtgacct ggtacagtgt tgaacaccac
    13441 aggcaaaacc ataatgcgtt gccagtgcct tttcttcttc gttattccag tagtagtaaa
    13501 gcgatattgt gccggtaaat ttctttttcc ccgcatttaa aacaaatctt gcgtcatcag
    13561 caacctgaat attgtgccgg ttaagtgctg gatcattgag catcttaacg atgtcatcag
    13621 caccatctgt gataaggtaa tccagcgatt cccctacaaa aacatagcct tttgttccat
    13681 tactatcctt tgcctgcgat agccccgtga tggtgtctga ctgcgattga tactttcctg
    13741 aagtaccctt tgcggtatgg gaaaccagag catacgagat acagcctgaa agcatcagtg
    13801 ctagtgggaa taaacaggca gccttaaaaa atcccttaaa attcaacttc atgattaatc
    13861 ctttaaacga gtagctaagc cgttgaaagc atactcttct ttccctcctg tttagcgcct
    13921 gatctccctt ttcagatttc tgcccgatat aagacctttt cttatgaggt gtttttttgt
    13981 ggactaatat ctgttgcaag gctatggctt cgccccggca ctggtttata tctccaccgt
    14041 cgcgttgatt ggtgcgctct cttacatcct gctggtgggc gatgtgaagc gcgttggcta
    14101 atccttcaac tgtggaatat gtgccacacc actgcgcata atccactttc ttaaagccgg
    14161 tgcggtcagg catcggcttt ttactatctt gccgaagagt tttaaacgca gtttgcgggt
    14221 ggcgcgccag taggctgcgt tgatcttctc tggctgcttc agtaacaccg aacgcagaat
    14281 ctctgcgcta tccagcgcat agcttatccc ctccagcgag ctggcgctga taaatcccgc
    14341 cgcttcacca atcagaaagg cgttgtcctt accgcagata aaatcctgcc agcgtgaggg
    14401 aaacagcact gtgcattttt cgcttttcac cgctttgcca aactggaact gaaaggcgct
    14461 cattttctct ttcagcgtcg cgaaacgcgt ctgaccgtct ttcattggat aggcaccacc
    14521 aaagataaaa taaccgtctt tgctgatact ccatgagtaa cagtcagtta tcgcattatc
    14581 aaagatgcag gagtagaacg gcaccggatg tttctctgcg aaccactgct ggattgcgac
    14641 atatttacgg atttgatgat ccgggtagag atgtcgccgc accatcgagt tggcaccatc
    14701 ggcaccgacc agatagcggg cggtaatatg ctgctcccag ccgtctgcac gaaaaatgac
    14761 atgccattta ttatcctcac gccagatttt ccggcacagg ctatcgtggt aaacctcaac
    14821 gctggcgggg atcagcgatt tcatccacaa gtcgaaagca tggcgattaa tattgatata
    14881 gcttcgctgg tagttacgcg tgagcgatgc ggcgacgtcg acggttttga cgctgaaaat
    14941 ctgcggatta gcgatcacat cgacaggaag cgtcagtcca tcgcgaataa aagatcgctg
    15001 cgcgtccggt gccagcagac cgccgcaggg tttgctgaaa ccttcagtac cacactggtg
    15061 ctttttatcc agcgcgatca cctgcatttt gcctgctaac tttcgtgcca acgccgatcc
    15121 tgccgggccg aggccaataa tcgccacgtc gaaatgttcc atcttttcac tcacattcat
    15181 cacgatggca aaagactatt gtctgaaagt gaagtccttg tgaagtatga tggcgtgaac
    15241 cgttttgtat caggaccacg ccatgaaaca gataaccttt gctccccgta atcacctgct
    15301 caccaatacc aatacctgga cgcccgacag ccagtggctg gtatttgacg tgcgtccttc
    15361 aggggcgtcg tttaccggcg agaccattga gcgtgtgaat atccatactg gagaggtcga
    15421 ggttatctat cgcgcgtcac agggcgcaca cgtcggcgtg gtgaccgttc atccgaagtc
    15481 agaaaaatat gtcttcattc acggcccgga aaatcctgat gaaacatggt attacgattt
    15541 ccatcatcgg cgcggagtca ttgctgaagg cggcaaggtg agcaatctcg atgcaatgga
    15601 tattactgca ccgtacaccc caggagcgct gcgcggcggc agccatgtgc atgtctttag
    15661 cccgaacggt gaaatggtga gctttaccta taacgaccat gtaatgcatg aacgcagccc
    15721 ggcgctggat ttgcgaaacg tcggggttgc tgcaccgttt ggcccggtca acgtacaaaa
    15781 gcagcatccg cgtgaataca gcggtagcca ctggtgcgca ctggtgagta aaacaacgcc
    15841 cacgccgcag cctggcagcg atgaaatcaa tcgtgcttat gaagaaggat gggtaggaaa
    15901 tcacgcgctg gcgtttattg gcgacacact ttcgccaaag ggcgagaaag taccggagct
    15961 gtttattgtc gagttgccga aagatgaagc tggctggaaa gcagctggtg atgcgccgtt
    16021 aagcggaacg gaaacaaccc tgcccgcgcc accgcgtggc gtcgtgcagc gacgtttaac
    16081 ctttacccac catcgggctt atccggggtt agtcaacgtc ccgcgccact gggtgcgctg
    16141 taatccgcag ggtacacaaa tcgcgttttt aatgcgagat gataacggca ttgtgcaact
    16201 gtggcttatc tcgccacagg gcggcgagcc gcgccagtta acccataaca aaacggatat
    16261 tcagtctgca tttaactggc atccatcagg agaatggttg ggctttgtgc tggataatcg
    16321 aattgcttgt gcccatgcgg aaagcgggga ggttgagtat ttaaccgaaa accacgccaa
    16381 tccaccttct gcggacgccg tggtcttctc gccggatggt caatggctgg cgtggatgga
    16441 aggcggtcag ctgtggatca ccgaaactga tcgctaaatc agttatcagg cattctggcc
    16501 ggtggaataa ccgcattggt ggcttgcgcg ttggcttcgc ttttttcaac gcgtgagcgc
    16561 accgagctgt ctttgcggaa cacatcccac ggcagtaaaa gtgtgtccat cacagcggta
    16621 aacggcatat cgagaatcgc cagcgactta gtgccccagt tggtttcatc gtcgccaatc
    16681 atgctggcgc tggcgcgggt gccgggatac gttccttctt tccccccggt atgggacatc
    16741 acgctggagc atcctccgag taatgtcatt ccgctgcata tcatgaacgc taacaacaca
    16801 tttcttatca taataaattc atctgttgat cgtgggtgtt ggcctgatga gttatagcga
    16861 tcccttgctg aaaataacat catcattacg tcgcactgtg gcggctatcg cactttaacg
    16921 tttcgtgctg ccccctcagt ctatgcaata gaccataaac tgcaaaaaaa agtccgctga
    16981 taaggcttga aaagttcatt tccagaccca tttttacatc gtagccgatg aggacgcgcc
    17041 tgatgggtgt tctggctacc tgacctgtcc attgtggaag gtcttacatt ctcgctgatt
    17101 tcaggagcta ttgattatgc gtaactttga tttatccccg ctttaccgtt ctgctattgg
    17161 atttgaccgt ttgtttaacc acttagaaaa caaccagagc cagagtaatg gcggctaccc
    17221 tccgtataac gttgaactgg tagacgaaaa ccattaccgc attgctatcg ctgtggctgg
    17281 ttttgctgag agcgaactgg aaattaccgc ccaggataat ctgctggtgg tgaaaggtgc
    17341 tcacgccgac gaacaaaaag agcgcaccta tctgtaccag ggcatcgctg aacgcaactt
    17401 tgaacgcaaa ttccagttag ctgagaacat tcatgttcgt ggcgctaacc tggtaaatgg
    17461 tttgctgtat atcgatctcg aacgcgtgat tccggaagcg aaaaaaccgc gccgtatcga
    17521 aatcaactaa ttccctaagg ccgccttgcg cggcctgaca tctccatgct cgccgtcagg
    17581 gagcatatgc gaatcttcgg atttgcaggt acttactcgc ttcttagaag gagaaatgac
    17641 tatgcgtaac ttcgatttat ccccactgat gcgtcaatgg atcggttttg acaaactggc
    17701 caacgcactg caaaacgccg gtgaaagcca gagcttcccg ccgtacaaca ttgagaaaag
    17761 cgacgataac cactaccgca ttacccttgc gctggcaggt ttccgtcagg aagatttaga
    17821 gattcaactg gaaggtacgc gcctgagcgt aaaaggcacg ccggagcagc caaaagaaga
    17881 gaaaaaatgg ctgcatcaag ggcttatgaa tcagccattt agcctgagct ttacgctggc
    17941 tgaaaatatg gaagtctctg gcgcaacctt cgtaaacggt ttactgcata ttgatttaat
    18001 tcgtaatgag cctgaaccca tcgcagcgca gcgtatcgct atcagcgaac gtcccgcgtt
    18061 aaatagctaa ctagctattc tctttgcccc gccattctgg tggggctttt tttgtctttc
    18121 ttcttctaca atgcgttaaa tttcgactgt ttaagatatt tcggcacgtt ctttcccgtg
    18181 ataatgtgcg ctataaggca aatctgcttc accgattcag ccataatccg tagcaattaa
    18241 aatgttatct caagagaaat ggacgatgag tgatatagca ttaacggtca gtattctggc
    18301 tttggtggca gtcgtcggtt tgtttatcgg caacgtcaaa tttcgcggca taggattagg
    18361 tattggcggc gtgctgtttg gtgggatcat cgtcggccat tttgtttctc aggcggggat
    18421 gacattaagt agcgatatgc tgcatgttat tcaggaattt ggtctgatcc tgttcgttta
    18481 tactatcggg attcaggtag ggccgggctt ctttgcctca ttgcgcgtct ccggattacg
    18541 cctcaacctg tttgctgttc tgatcgtcat catcggtggt ctggttaccg ccatcctgca
    18601 taaactgttt gatattccac tgccggtagt gctggggatt ttctccggtg cggtaaccaa
    18661 tacgccagcg ctgggggcag ggcagcagat cttgcgcgac ctgggtacac caatggaaat
    18721 ggtcgatcag atggggatga gttacgcgat ggcgtatcca ttcggcattt gcgggatatt
    18781 gttcaccatg tggatgttgc gggttatttt ccgcgtcaat gtcgagacag aagcccagca
    18841 gcacgaatct tcacgcacca atggcggcgc gctgatcagg actatcaata ttcgcgttga
    18901 gaaccctaac ctgcatgatt tagccattaa agatgtaccg attctcaacg gcgacaaaat
    18961 tatctgctcg cgtctgaaac gcgaagaaac cctaaaagtt ccttcgccag ataccattat
    19021 ccaactgggc gatttgctgc atctggtggg tcagccagcg gatttacata atgcgcaact
    19081 ggtgattggt caggaggtcg atacttcgct gtccacgaaa ggcactgatt tgcgcgtcga
    19141 gcgtgtggtg gtcaccaatg aaaacgtgct tggtaagcgt attcgcgacc tgcattttaa
    19201 agaacgctat gacgttgtta tctcgcgcct gaaccgtgcc ggggtcgaac tggtcgccag
    19261 tggcgatatc agcctgcagt tcggcgatat cctcaacctg gtggggcgtc cgtccgcaat
    19321 tgatgccgtt gccaatgtgc tggggaatgc gcagcaaaaa ctgcaacagg ttcagatgct
    19381 gccagtgttt attggcatcg ggcttggcgt attgttaggt tctattcccg tctttgtgcc
    19441 tgggttcccg gccgcgttga aactggggct ggcgggcggt ccgctgatta tggcgttgat
    19501 cctcgggcgt atcggcagta tcggcaagct gtactggttt atgccgccaa gtgccaacct
    19561 cgcgctgcgg gagctgggga tcgtgctgtt cctctcggtc gttggtctga aatctggtgg
    19621 ggattttgtg aataccttgg tcaatggcga agggctaagc tggattggtt atggtgccct
    19681 gatcaccgcc gttccgctga ttactgttgg cattctggcg cggatgttag ccaaaatgaa
    19741 ttacctgacc atgtgcggga tgctggcagg ttccatgacc gatcctccgg cgctggcgtt
    19801 tgctaataat cttcatccaa ccagcggtgc ggcggcgctc tcttacgcca ctgtctatcc
    19861 gttagtgatg ttcctgcgca ttatcacccc ccaattactg gcggtgctct tctggagtat
    19921 cggttaactc ttgtccgggt ggagtcgccg taaatggtac tccacctggt attcgctggc
    19981 atttctgaac atcactgaat aattcaaaaa ctccccgctc tcgctatatg acaacgaggt
    20041 gatccgcagc agtggcgtgt gttccgctac cttcatttgc cgcgccagca gtctatcggc
    20101 gagcaccggc gtcaggcttt cataattacc gccaatcaga atcccgcact cttgttcaat
    20161 atattcaaac ttcgaccctt ccagatgttg cagcgaaaga ttacggaaca gttttaccgg
    20221 catatagctg tcttccagca tcagcggttt cccttccaca aaacgaacgc gacgggagaa
    20281 gtagatctgc tcgttgattt gaatccgtaa ctggctggca attgccggag gcgcaggcat
    20341 gatttcaaaa atcagcacct ggctggtgac tgtttttccc tgccgtttta acacctccac
    20401 cagcccggtc aggctggcgg tttgatgcag tacatctttg cgcaccaggt aagtgccgct
    20461 gccgtggcgg cggaccacca gcccccacgc taccagcagg tcaatggctt tacggatggt
    20521 catccgcgat accgcaaact cttccgccag ctttttttca ccgggaagaa ggctgtttag
    20581 cgtgaaatct gcggaattaa gtctaattct taaccgctcc gcaatgcttt tgtagatcat
    20641 ctctagatct cttttttgcc tgcaagcccg ggtaatgcgc ccacaagttc tgcttaatcg
    20701 attgaaaaaa catctttttt ttaaagatgt gttcgatgct gtgtacctgt gctcacagag
    20761 gtctactttt tcgcgaaaac gtagatctct accgcccaac gaaaagcatg aaagcgatca
    20821 cgaatcccat tgggtcgtca tgttctgccg atcgcatctt cctatcctcg ctccaggcct
    20881 gccgcataac caatcaggct tcctacttac agaattgaga aaagaggatg tggaaatgct
    20941 cagtcaaatt caacgctttg gcggcgcgat gttcacgcca gtgctgctgt ttcccttcgc
    21001 cgggattgtg gtgggtcttg ccatcttgct gcaaaacccg atgtttgtcg gggaatcact
    21061 gaccgatccg aacagtttat tcgcgcaaat cgtacacatt attgaagagg gcggatggac
    21121 ggtattccgt aatatgccgc tgatttttgc tgtcggttta cccattggcc ttgctaagca
    21181 agcgcagggg cgtgcttgtc tggcggtgat ggtgagtttc ctgacctgga actatttcat
    21241 caacgcgatg ggaatgacct ggggaagcta cttcggcgtc gatttcactc aggacgcggt
    21301 ggcaggtagc ggtctgacaa tgatggccgg gattaaaacc ctcgatacca gcattatcgg
    21361 cgcaattatc atttccggca ttgtgacggc gctgcataac cgtctgttcg ataaaaaact
    21421 gccggtgttt ctcggcattt tccaggggac gtcttatgtg gtgattatcg ccttcctggt
    21481 gatgatcccc tgtgcctggc tgacattgct cggctggcca aaagtacaaa tggggattga
    21541 atctctgcaa gcgttcctgc gttcggcggg tgcgcttggg gtgtgggttt acaccttcct
    21601 cgaacgtatt ctgatcccaa ccggtttaca ccacttcatc tacggaccgt ttatctttgg
    21661 tccggcagct gttgaaggcg gcattcagat gtactgggcg cagcatctgc aagagttcag
    21721 tttgagcgcc gagccgctga aatcgttgtt cccggaaggc ggttttgccc tgcacggtaa
    21781 ctcaaaaatc tttggtgccg tgggcatttc tttagcgatg tacttcactg ccgcaccgga
    21841 aaatcgggta aaagtggcgg gcttgctgat tcccgcaacc ttaaccgcca tgctggtggg
    21901 aattaccgaa ccgctggaat ttaccttcct gttcatttca ccgttgctgt ttgcggtaca
    21961 cgctgtgctt gcggcctcaa tgtcgaccgt gatgtatctc tttggtgtgg tgggcaacat
    22021 gggcggaggt ctgattgacc aggttttacc gcaaaactgg atcccgatgt tcagcaacca
    22081 cgcggatatg atgttgaccc aaatcgccat tgggttgtgc tttaccctgc tgtacttcgt
    22141 ggttttccgc accctgattc tgcaattcaa catgtgcacg ccgggacgtg aagatgcgga
    22201 agtgaaactc tactcaaaag ccgaatacaa agcctcgcga ggccaaacca ccgcggcaga
    22261 gccaaaaaaa gagctggatc aggctgccgg tatcctgcaa gccctgggcg gggtcggcaa
    22321 tatctccagc atcaacaatt gcgcgacgcg tctacgtatt gcactgcatg acatgtcaca
    22381 aacgctggat gacgaagtct ttaaaaagct gggagcgcac ggcgtcttcc gtagtggcga
    22441 tgccattcag gtgatcattg gtctgcatgt atcccagctg cgtgaacagc tcgatagctt
    22501 aattaattct catcaatcag cagaaaatgt tgccattacg gaggcagtat aatgaccaaa
    22561 ttctcagtgg ttgtcgcagg cggtggaagc acctttacgc caggcatcgt gttgatgctc
    22621 ctggcgaatc aggaccgttt cccgcttcgt gcactgaaat tttatgataa cgatggcgcg
    22681 cggcaggaag tgattgccga agcctgtaaa gtcatcctta aagagaaagc gccggacatt
    22741 gcgtttagtt acaccaccga tcctgaagtg gcgttcagcg acgttgattt tgtcatggcg
    22801 cacatccgcg tcggcaaata cccgatgcgc gaactggatg aaaaaatccc gctgcgccac
    22861 ggcgttgttg gtcaggaaac ttgcggaccc ggcggaatag cgtacggcat gcgttccatt
    22921 ggcggcgtcc tggaactggt ggattatatg gaaaaatatt caccaaacgc ctggatgctc
    22981 aactactcca acccggcagc cattgtcgca gaagccacgc gtcgtctgcg cccgaatgcg
    23041 aaaatcctca acatctgtga catgccaatc ggtattgaaa gccggatggc gcaaattgtt
    23101 gggctgcaag atcgcaaaca gatgcgcgtg cgctactacg gcctgaacca ctttggctgg
    23161 tggacatcaa ttgaagattt gcagggcaac gacctgatgc cccagctgcg gcaatatgtc
    23221 tcgaagcacg gttatgttcc accgcagcaa gatacacata ctgaagcgag ctggaacgac
    23281 acctatgcaa aagcgcggga tgtccaggca ctggacccgg atacattacc aaacacctat
    23341 ctgaaatatt atctcttccc ggattatgtc gttcagcatt ccaaccctga acatacccgc
    23401 gcgaatgagg tgatggaaca tcgcgagaaa caggttttcg atgcttgccg cgccattacg
    23461 gcggcaggaa attcagcggc gggcaagctg gagattgacg aacatgcgtc atacatcgtc
    23521 gatctggcgg cggcaattgc cttcaacact caggagcgga tgttgctgat tgtgcctaac
    23581 aacggggcaa tccataactt tgatgatgaa gcgatggtcg agatcccgtg tctggtcggg
    23641 cacaacggac cagaaccact ggtggtcggc gatatcccgc agtttcagaa agggttaatg
    23701 agtcagcaag tggcggtgga aaaactggtc gtggacgcct gggaacagcg ttcatatcag
    23761 cacctgtggc aggcgattac gttgtcgaaa acggtaccga gcgcctcggt cgccaaagct
    23821 attctggatg agttgctgga ggccaacaaa gcgtactggc cagagttacg ttaattgtgt
    23881 ggactggcat ccgttgggtg ctggtccgta ttctttagca cggctgaagg cgcataacca
    23941 aatttatgtt taaaggcttt gctgaaatga aaggagtcga agaagttgag cgtatcagcg
    24001 acttcgccaa ccgagtttcc ttgttgcttt aatagcgata gcgccagatc cagacgcgca
    24061 tcgaggtaat attctttcgg cgtcttgccg gtatagcgta aaaacagacg acgcaaccag
    24121 gcttcgctac aggggatcgt ggcagccata tcggctacgc tccagcgttg ttgcagactg
    24181 gcatgtaaag tggcaattaa tttttcaatt tgccgccgtt gtggatcttt tttaccgtct
    24241 gcgtacataa gacaaatcca ctgataaata attttcgtca gaaaagcgac tgccagatta
    24301 ttttttattg cttctggtga agttattaac tcagcaactt cggtgagttc ctgattataa
    24361 atttcgccat tataaataac gctttgctga ccaacgggaa tatccatcat actggtgggg
    24421 gtaaattcca tccagtactg ttcccagact aaaccttcac agtgataaga gtgaatatcc
    24481 attggcttta aaaatataat acagttgcca tgcagagtta tttgttcgcc attctttaaa
    24541 agaattttcc cacatccttg tacggtatac accgcaaccc atgagttggc gataagcggt
    24601 ttcttcttgt cccgcggcca gatcacccgg taatgctcgt cggcgtaggt atgccagagc
    24661 gagatcagag aaattccgct ggagatcaca ttttcgttaa tcaataaggg aatattgtat
    24721 ggagtttcgt tttttacatc tgaagtttgc aattttccat tcattttaat atccctccgg
    24781 cgaattatgc tcacaacctg aacttaacag aaaacaatcc gttcagggat acgacgtttg
    24841 tattttaaga atctgactgc ctgacccgac gcattttacc tctccctata ttcatgcgtc
    24901 cgggacataa acataaataa gggctatgag atgaattcgt tacaaatctt gagttttgtc
    24961 ggttttacgc tgctggtggc gatcatcacc tggtggaagg ttcgcaaaac agataccgga
    25021 tcgcaacaag gctattttct tgccggacgt tcactaaaag cgccggttat tgctgcttcg
    25081 ttaatgttaa ccaacctttc cacggaacaa ctggttggtc tttccgggca ggcctacaaa
    25141 agcggcatgt cggtgatggg ctgggaagtg acttcagcgg tgacgctgat cttcctcgcg
    25201 ctaatctttt taccgcgcta tctgaagcgc ggcattgcca ccatccccga ttttctggag
    25261 gaacgttatg ataaaacgac gcgtattatc atcgacttct gcttcctcat tgccaccggc
    25321 gtctgctttc tgccgattgt tctctactcc ggcgcgttgg cgctcaacag cctgtttcac
    25381 gtcggggaat cgctacagat ttctcacggt gcggctatct ggctactggt aattttgctt
    25441 ggtctggcgg gaattttgta tgcggtgatc ggcggactgc gcgcaatggc agtggcggac
    25501 tccatcaacg gtattgggct ggttatcggc gggttgatgg tgccggtatt tggcctgatc
    25561 gcgatgggca agggcagctt tatgcagggc attgagcaaa ttaccaccgt tcacgccgag
    25621 aaattaaact cagtcggtgg cccgaccgat cccttgccga ttggcgcggc atttaccggt
    25681 ttaattctgg tgaacacctt ttactggtgt acaaatcagg gcatcgtgca acgcacgctg
    25741 gcgtcaaaaa gcctggcgga agggcaaaag ggggcgctgt taacggcggt gctgaaaatg
    25801 ctcgacccgc tggtactggt gctgccaggg ttgattgcgt ttcatctgta tcaggatcta
    25861 ccgaaagccg acatggccta cccgacgctg gtcaataacg ttctgccagt gccactggtg
    25921 ggtttcttcg gcgcggtgtt atttggtgcg gtgatcagta ccttcaacgg ctttctgaat
    25981 agcgccagta cgttattcag tatgggtatt taccgtcgca tcattaacca gaatgccgag
    26041 ccgcagcagc tggtcaccgt cgggcgcaaa tttggtttct ttatcgctat cgtttcggtg
    26101 ctggtagcgc cgtggatcgc caacgcgccg caggggctgt atagctggat gaaacagctc
    26161 aacggcattt acaacgtgcc gctggttacc atcatcatta tgggcttttt cttcccgcgc
    26221 atcccggcgc tggcggcaaa agtggcgatg gggattggca taatcagcta catcaccatc
    26281 aactatctgg tgaagttcga cttccatttc ctctatgtgc tggcctgtac gttctgcatc
    26341 aacgtggtcg tgatgctggt gatcggtttt atcaaaccgc gcgccacgcc gttcaccttc
    26401 aaagatgcgt ttgcggtgga catgaaaccg tggaaaaacg tcaagatcgc gtcaattggc
    26461 atcctgttcg cgatgattgg cgtctatgcc gggctggctg aattcggcgg ctacggtacg
    26521 cgctggttag cgatgatcag ttatttcatt gccgccgtag tgattgtcta cctgattttt
    26581 gacagctggc ggcatcgtca cgacccagcc gtaaccttta ctcccgatgc aaaggatagc
    26641 ctatgaaacg ccccaatttt ctgttcatca tgaccgatac ccaggccact aatatggtcg
    26701 gttgctatag cggtaagccg ctgaatacgc aaaatattga tagtctggcg gcggaaggta
    26761 ttcgctttaa ctccgcctac acctgttcac cggtttgtac accggcgcgc gccgggctgt
    26821 ttaccggtat ctacgctaac cagtccggcc cgtggaccaa caacgtcgcg ccaggcaaaa
    26881 acatttccac tatggggcgc tactttaagg atgcgggcta tcacacttgt tacatcggca
    26941 aatggcatct cgacggtcat gactatttcg gcactggcga gtgtccgccg gagtgggacg
    27001 ctgattactg gttcgatggg gcgaactatc ttagcgaact gacggaaaaa gagattagcc
    27061 tgtggcgcaa tggcctaaac agcgtcgaag atttacaggc gaaccatatt gacgaaacct
    27121 tcacctgggc gcaccgcatc agcaatcggg cggtggattt tctgcaacag cccgcgcgca
    27181 ccgaggaacc cttcctgatg gtggtttcgt atgatgagcc gcatcacccg ttcacctgtc
    27241 cggtggagta tttagagaaa tacgctgatt tttactacca actgggcgag aaagcacagg
    27301 atgacctggc gaacaaacca gaacatcacc gcttatgggc gcaggcgatg ccatcgccag
    27361 tcggtgatga cgggctttat caccatccgc tctattttgc ctgtaatgac tttgttgatg
    27421 accaaatagg acgggtcatc aatgccttaa cgccagagca acgtgaaaat acgtgggtta
    27481 tttatacatc cgatcacggc gaaatgatgg gcgcacataa gctgatcagt aaaggagcgg
    27541 cgatgtatga cgacatcacc cgtattccgc tgatcatccg ttcgccgcaa ggggagcggc
    27601 gacaggtcaa tacgccagtc agtcatatcg atttactgcc gacaatgatg gcgctggcag
    27661 atattgaaaa accagagatt ctgccggggg aaaatatcct tgccgtgaaa gagccacgcg
    27721 gcgtgatggt ggaatttaac cgctacgaga ttgagcatga cagctttggc ggttttattc
    27781 cggtgcgttg ctgggtgacg gatgacttta aactggtgct caacctcttc accagtgatg
    27841 aactttacga tcgccgtaat gacccaaatg aaatgcataa cctgatcgat gatgcccatt
    27901 ttgccgacgt tcgcagcaaa atgcatgatg ccttattgga ttatatggac aaaattcgcg
    27961 atccgttccg cagctaccaa tggagcctgc gtccgtggcg taaagatgca cggccgcgct
    28021 ggatgggggc gtttcgtcca cgcccacaag atggctattc gccagtggta cgtgactatg
    28081 acaccggcct gccgacacaa ggggtgaaag tggaggataa aaaacagaag ttctgacgat
    28141 aatgcccgct gatgatcacc cggcgggcat tattcaggct gcttaaacca gtgaaccgcc
    28201 ggtcaggaag gaaaagataa ggaatatcag caaaatgctg cacacgatgc cgataccaaa
    28261 aagcaaggta tggaaagcca gcgtcccgcc gccaaaaacc agcgcgccgc gaatgcccca
    28321 gcgattttcc ttacgcatac cgccaataaa tgcgcaaagt agcgcagcaa tggccagtgc
    28381 gataccgctt tgatcgagga ttttatcgac atctacgtcc tcttttttct ccacggtggt
    28441 aattttttcg ccttttattc cggcgagaag acctctttta atttcagccg ttttgtccgc
    28501 cacgatgctt tcaagcgttg gcggtggtgc ggagaaaggg ccgaatgaaa agtggataat
    28561 ccccatcatt aaagcgatgg caccgaatag catgcccaat gaactgattt tattttgcgc
    28621 tatgataccc atctgacctc cttgtagcgg atggcaaaag catatcagga aacgggcgga
    28681 cgaaattctt aaaaaaaacc gctaaaggta gagcgttagt gaaattgcaa aggagcaaca
    28741 atgaaaattt cccgcctcgg agaagcgccc gattaccgct tctcgctggc taatgagcgt
    28801 acttttctgg cgtggatccg tactgcacta ggatttctgg cggcgggggt tgggcttgat
    28861 caacttgcgc cagatttcgc cacgcccgtc attcgcgaac tgctggcgtt gttgttgtgt
    28921 ctgttttccg gcgggttagc gatgtatggc tacttgcgct ggttacgcaa tgaaaaagcg
    28981 atgcgtctga aagaggacct gccctacacc aacagcttat taatcatcag cttaatattg
    29041 atggtcgtag cagtgatcgt tatgggactg gtgttgtatg ccggatagcc gcaaagccag
    29101 acgcattgcc gacccagggc tgcagccgga gcgtacatca ttggcgtggt ttcgtaccat
    29161 gctgggctac ggcgcgttga tggcgttggc tatcaaacac aactggcacc aggcgggcat
    29221 gttattctgg atttcgattg gcatcctcgc catcgtggcg ctgatcctct ggcactacac
    29281 tcgtaatcgc aatttaatgg atgtcacgaa tagcgatttt tcccaatttc acgtagtccg
    29341 tgacaaattt ttgatctccc tcgcggtgtt atctctcgca atactgtttg ctgtaacgca
    29401 tatacatcaa cttatcgtat ttattgagag agtcgcatga caggaagtca ggtcattgat
    29461 gctgaagagg acaggcataa actggttgta gagtataagg atactctgca acctgccgat
    29521 ttttatcaca atttcaaaca gcggggcatc cgctccgtac agcttattcc ccatcttgaa
    29581 tttgatgacc ggggcgatat gacggccgct tcggtgacag cagagctgtg ggggaaattt
    29641 ttaattgccc tgtttgagtg ttgggtgcgg gcagatatca gtcgtatttc gattgagctt
    29701 tttgacgcca ctttacaaaa atggtgtggc agcgaaaacc cgcacccgcg ccgtgactgc
    29761 caggcgtgcg actggcatcg gctgtgtccg cacgctcggg aggagacgcc agacaacgtg
    29821 ctttgtgcgg gctatcaggc gttttattct tactccgcac cgcatatgcg ggtgatgcgt
    29881 gatctgataa agcagcatcg ttctcccatg gagctgatga cgatgctgcg gtgatgtgcg
    29941 cttaaacggg ctgcccctga tgcgacatcc gcgtcgccag cggtagccag cacagcacga
    30001 tcaacaatcc cattaaggtc atcaacaacc ccaggctgcc ctgaccggtt tgcggcaaca
    30061 tcgctgagag cgacgccagc acgccggaac caatgttttg cagaccaccg accagcgcgc
    30121 cagccgtgcc cgccaggaag gggaacggct ccatcgcgcc gctggtcgcc agcggaaaca
    30181 gcatcccggc accgaaaaag aatagcgcgg cgggaacaag cagcgtccag acattcatca
    30241 cgccaaacca gtcggggatc cacatcagca agccagccag caggcagcag ataaccgact
    30301 gccacatcaa cgttgagaag cgtttattag gacgcccggc aaaccatgcc ccaaaaaatg
    30361 ccgccggaat cggcagaata aacaaaatac tgaccgtcat actgctcagc cctaacaccg
    30421 cgcccatcag cacgccggag caggcttcaa aggcggcaat cccggccaga ccgccaatca
    30481 gcatcagcaa ataacagtta aaaccgccgt taccgaaaag cgttttataa ctggtaagca
    30541 ggcgcgtgcg cggtgcatcg accggacgcg tttccggcat ccagcgggcc atactgaagg
    30601 tcacgccagc acaaagaacc aacaaaaaga gataacaggc gcgccagttc cacatcgtat
    30661 ccagcagacc gccgattagc ggtgcgagca acggactaac aagaatcccc atgtttaaca
    30721 ggctgttagc atggcgcaac tgtgtccgtt catataaatc acgcggcaaa gtacgcgcca
    30781 ttacgccgcc aacgccggtg cccatcccct gcatcgcgct ggcggcaatc aataccgtca
    30841 aactggaggt cgtgaccgcg accagcgttg ccagcataaa aatggacatt ccgacgagga
    30901 tcaccggtcg gcgacccaca cggtcggaaa tcgggccata aaacagctgt gagacaccgt
    30961 aagtcagcag ataagcgccc attacgctct gcaccgcccc ttcacggacg ttgagatcgc
    31021 gcgccatatc ggcaatagct ggaatataaa tggtttgcgc catctgaccg acggccacga
    31081 gtaataccaa catcaataac aaattgacgt ttctatgcct tttcattatc acggatgctt
    31141 ttataaaaat aattagaata agtcactacg cattcccata aatgcgggag aggaatctat
    31201 cacattgatg aatgttagcc agatatgtgc caggaatggc gaaaagaaaa acggcagaat
    31261 atgtaaacaa aagcggcaat aaatgttgcc ggaagggatg gtaagtcgga taagatgcgt
    31321 cagcatcgca tccgacacca acccacaagc agaatataaa aggggagcgg tttcccgctc
    31381 ccctttggtg cgacttgaat ctgaattact tcaggtattt cagaacagca tcaagtagtt
    31441 gcagtgctgc aacaatgagt ttgaggataa gaatggcgat atccaccagg ctcatacgcg
    31501 tctcctgtgg ttcaggagca ctgccagctg acgtaccttt ccgctgcctg ttgccagctg
    31561 ttgtgcacgc ggtatctcaa ggagaaacgt tgtgcttgtt gacataacac agtgtgctca
    31621 cgcggggtta aggcgctgac ggcaccaccc gtttcagcta ggacttagtg cgccgggtaa
    31681 cgatgcggcc cccgcataac acattgcgta cagtgatatt atagaagttt actgtataaa
    31741 taaacagtaa tagttggaca aaacgcaaac tgtgatcgat actcatctat gcactaaata
    31801 cgtcaaaatt cgtgccgaaa ttgcgcgttc tgcgcggaac aggtatactt tcagtgttga
    31861 cataatacag tgtgctttgc ggttaccagc cgcaggcagc tgacgaaacc tcgctccggc
    31921 ggggtttttt gttacctgca attcagtata aaacgtgatc aacccctcaa ttttcccttg
    31981 ctgaaaaatt ttccattgtc tcccctgtaa agctgtgctt gtataaatat tgttaaacac
    32041 aaaaccaaca aggtccccaa tgactacttc catgttcaac gcaaagctac taccaactgc
    32101 gccatccgcc gcagtggtcg tcgtgcgtgt ggtggtggtc gtcggcaatg cgccgtaggg
    32161 actggaacaa cacacgattc caaaaccccg ccggcgcaaa ccgggcgggg tttttcgttt
    32221 aagcacctcc cggaaagtcg gcccagaaaa aaaggactgg agcatggcaa gttcgggcac
    32281 aacatcgacg cgtaagcgct ttaccggcgc agaatttatc gttcatttcc tggaacagca
    32341 gggcattaag attgtgacgg gcattccggg cggttctatc ctgcctgttt acgatgcctt
    32401 aagccaaagc acgcaaatcc gccatattct ggctcgccat gaacagggcg cggggtttat
    32461 cgctcaggga atggcgcgca ccgacggtaa accggcggtc tgtatggcct gtagcgggcc
    32521 gggtgcgact aacctggtga ccgccattgc cgatgcgcgg ctggactcca tcccgctgat
    32581 ttgcatcact ggtcaggttc ccgcctcgat gatcggcacc gacgccttcc aggaagtcga
    32641 tacctacggc atctctatcc ccatcaccaa acacaactat ctggtcagac atatcgaaga
    32701 actcccgcag gtcatgagcg atgccttccg cattgcgcaa tcaggccgcc ctggcccggt
    32761 gtggatagac attcctaagg atgtacaaac ggcagttttt gagattgaag ctcagcccgc
    32821 ggtggcagaa aaagccgctg cacccgcctt tagcgaagaa agcattcgtg acgcggctgc
    32881 gatgattaac gccgccaaac gcccggtgct ttatctgggc ggcggtgtga tcaatgcgcc
    32941 tgtgcgggtg cgtgaactgg cggagaaagc gcaactgcca actaccatga ccttaatggc
    33001 gctgggtatg ctgccaaaag cgcatccgct gtcgctgggt atgctgggga tgcacggcgt
    33061 gcgcagcacc aactatattt tgcaggaggc ggatttgttg attgtgctcg gtgcgcgttt
    33121 tgatgaccgg gcgattggca aaaccgagca gttctgcccg aatgccaaaa ttattcatgt
    33181 cgatatcgac cgcgcagagc tgggtaaaat caagcaaccg catgtggcga ttcaggcgga
    33241 tgttgatgac gtgctggcgc agttgatccc gctggtggaa gcgcaaccgc gtgcagagtg
    33301 gcaccagttg gtggcggatt tgcagcgcga atttccgtgt ccaatcccga aagcgtgcga
    33361 tccattaagc cattacggcc tgatcaacgc cgttgccgcc tgtgtcgatg acaatgcgat
    33421 tatcaccacc gacgttggtc agcatcagat gtggaccgcg caagcctatc cgctcaatcg
    33481 cccacgccag tggctgacct ctggcgggct gggcacgatg ggctttggcc tgcctgcggc
    33541 gattggcgcg gcgttggcga acccggatcg caaagtgttg tgtttctccg gcgacggcag
    33601 cctgatgatg aatattcagg agatggcgac cgccagtgaa aaccagctgg atgtcaaaat
    33661 cattctgatg aacaacgaag cgctggggct ggtgcatcag caacagagtc tgttctacaa
    33721 gcagggcgtt tttgccgcta cctatccggg aaaaatcaac tttatgcaga ttgccgccgg
    33781 attcggcctc gaaacctgtg atttgaataa cgaagccgat ccgcaggctg cattgcaaga
    33841 aatcatcaat cgccctggtc cggcgctgat ccatgtgcgt attgatgccg aagaaaaagt
    33901 gtacccgatg gtgccgccag gtgcggcgaa tactgaaatg gtgggggaat aagccatgca
    33961 aaacacaact catgacaacg tgattctgga actcaccgtt cgcaaccatc cgggcgtaat
    34021 gacccacgtt tgtggccttt ttgcccgtcg cgcttttaac gttgagggca ttctttgctt
    34081 gccgattcag gacagcgaca aaagccacat ttggctactg gtcaatgacg accagcgtct
    34141 ggagcagatg ataagccaga tagacaagct ggaagatgtg gtgaaagtgc agcgtaatca
    34201 gtccgatccg acgatgttta acaagattgc ggtgtttttt cagtaaggag cgacattaaa
    34261 ccgctcgggg gggtactgat gagcgatttt tgatagatcc gttcagatat atagggtgaa
    34321 attgtgccag agaccatctc aggcacaatt tattagccct gaatgttaat ttaagtcgct
    34381 gcaccagtaa tttgggggtt cacagttagg gctaaaattt ccagcgatgg tgttaaattc
    34441 tgattgctgt ctataccaat ggtcatgacc agcattctat taattaatgg ttcatcgtga
    34501 ggtatattat ggcaagccct ataaacagcg gaataatgat gtcaaatctc tgctcttcca
    34561 cctttttgga taaaaatata aatgtcgctg ctgagcttga tattacaaat agcgagaaaa
    34621 aatattcgcc cggcagtaat tttgcaaaat ggatgttgca ggaaataaag cgcttaatag
    34681 caaatattat gtccggttct cgcagtgtta acaccgaaat ccttaactat ttccacccta
    34741 tgccaggcac tgaaaataat ggcaatcgta cctggatggc ggcaactgga gagaacgaat
    34801 ataccgtaat cgaacaaaca ggtggcaaat cctttaaagt tatacagttt gatgcaggtg
    34861 aaaaaaatac aaaaaaaata gtaaaagaaa catcatattc tggagtggct gtggcgacta
    34921 ttatcaaatc gctgtctgaa aaaactgctg cgttagaaac acattctgct gacacggttt
    34981 tgag
//
LOCUS       DAZGCQ010000050        34810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000050 DAZGCQ010000000
VERSION     DAZGCQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.050"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..1048
                     /gene="mdtN"
                     /locus_tag="ACHK07_002596"
     CDS             17..1048
                     /gene="mdtN"
                     /locus_tag="ACHK07_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO8102910.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1048..3099
                     /gene="mdtO"
                     /locus_tag="ACHK07_002597"
     CDS             1048..3099
                     /gene="mdtO"
                     /locus_tag="ACHK07_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO8102911.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGVQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICHTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATGSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLTVALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3096..4562
                     /gene="mdtP"
                     /locus_tag="ACHK07_002598"
     CDS             3096..4562
                     /gene="mdtP"
                     /locus_tag="ACHK07_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO8102912.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKNSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVLQSIQLMKSLGGGYQAGPVVEKK"
     gene            4760..6907
                     /gene="fdhF"
                     /locus_tag="ACHK07_002599"
     CDS             4760..6907
                     /gene="fdhF"
                     /locus_tag="ACHK07_002599"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5177..5179,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO8102913.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7001..7690
                     /gene="yjcO"
                     /locus_tag="ACHK07_002600"
     CDS             7001..7690
                     /gene="yjcO"
                     /locus_tag="ACHK07_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO8102914.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7995..9308)
                     /gene="gltP"
                     /locus_tag="ACHK07_002601"
     CDS             complement(7995..9308)
                     /gene="gltP"
                     /locus_tag="ACHK07_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO8102915.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9650..10246)
                     /gene="nrfG"
                     /locus_tag="ACHK07_002602"
     CDS             complement(9650..10246)
                     /gene="nrfG"
                     /locus_tag="ACHK07_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO8102916.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10243..10626)
                     /gene="nrfF"
                     /locus_tag="ACHK07_002603"
     CDS             complement(10243..10626)
                     /gene="nrfF"
                     /locus_tag="ACHK07_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO8102917.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10619..12337)
                     /gene="nrfE"
                     /locus_tag="ACHK07_002604"
     CDS             complement(10619..12337)
                     /gene="nrfE"
                     /locus_tag="ACHK07_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO8102918.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12357..13313)
                     /gene="nrfD"
                     /locus_tag="ACHK07_002605"
     CDS             complement(12357..13313)
                     /gene="nrfD"
                     /locus_tag="ACHK07_002605"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO8102919.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13310..13981)
                     /gene="nrfC"
                     /locus_tag="ACHK07_002606"
     CDS             complement(13310..13981)
                     /gene="nrfC"
                     /locus_tag="ACHK07_002606"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO8102920.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13978..14544)
                     /gene="nrfB"
                     /locus_tag="ACHK07_002607"
     CDS             complement(13978..14544)
                     /gene="nrfB"
                     /locus_tag="ACHK07_002607"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO8102921.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14589..16025)
                     /gene="nrfA"
                     /locus_tag="ACHK07_002608"
     CDS             complement(14589..16025)
                     /gene="nrfA"
                     /locus_tag="ACHK07_002608"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO8102922.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16418..18376
                     /gene="acs"
                     /locus_tag="ACHK07_002609"
     CDS             16418..18376
                     /gene="acs"
                     /locus_tag="ACHK07_002609"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO8102923.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18509..18823
                     /gene="yjcH"
                     /locus_tag="ACHK07_002610"
     CDS             18509..18823
                     /gene="yjcH"
                     /locus_tag="ACHK07_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO8102924.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18820..20469
                     /gene="actP"
                     /locus_tag="ACHK07_002611"
     CDS             18820..20469
                     /gene="actP"
                     /locus_tag="ACHK07_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO8102925.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGCWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20647..21939
                     /gene="espX5"
                     /locus_tag="ACHK07_002612"
     CDS             20647..21939
                     /gene="espX5"
                     /locus_tag="ACHK07_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO8102926.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASYANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGT
                     LNSCSAMHVQFYNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSF
                     SKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQDAD"
     gene            complement(22093..23742)
                     /gene="yjcE"
                     /locus_tag="ACHK07_002613"
     CDS             complement(22093..23742)
                     /gene="yjcE"
                     /locus_tag="ACHK07_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO8102927.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23893..25242)
                     /gene="ghxP"
                     /locus_tag="ACHK07_002614"
                     /gene_synonym="yjcD"
     CDS             complement(23893..25242)
                     /gene="ghxP"
                     /locus_tag="ACHK07_002614"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO8102928.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25788..26252)
                     /gene="soxR"
                     /locus_tag="ACHK07_002615"
     CDS             complement(25788..26252)
                     /gene="soxR"
                     /locus_tag="ACHK07_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO8102929.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26338..26661
                     /gene="soxS"
                     /locus_tag="ACHK07_002616"
     CDS             26338..26661
                     /gene="soxS"
                     /locus_tag="ACHK07_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO8102930.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26664..28250)
                     /gene="pdeC"
                     /locus_tag="ACHK07_002617"
     CDS             complement(26664..28250)
                     /gene="pdeC"
                     /locus_tag="ACHK07_002617"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO8102931.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYAHDN
                     HFLCSSLIAPVNGYTIAPADYKRDPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            28679..28960
                     /gene="yjcB"
                     /locus_tag="ACHK07_002618"
     CDS             28679..28960
                     /gene="yjcB"
                     /locus_tag="ACHK07_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO8102932.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(29059..29595)
                     /gene="ssb1"
                     /locus_tag="ACHK07_002619"
     CDS             complement(29059..29595)
                     /gene="ssb1"
                     /locus_tag="ACHK07_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO8102933.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            29850..32672
                     /gene="uvrA"
                     /locus_tag="ACHK07_002620"
     CDS             29850..32672
                     /gene="uvrA"
                     /locus_tag="ACHK07_002620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO8102934.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(32707..33063)
                     /gene="yjbR"
                     /locus_tag="ACHK07_002621"
     CDS             complement(32707..33063)
                     /gene="yjbR"
                     /locus_tag="ACHK07_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8102935.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(33067..33483)
                     /gene="yjbQ"
                     /locus_tag="ACHK07_002622"
     CDS             complement(33067..33483)
                     /gene="yjbQ"
                     /locus_tag="ACHK07_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO8102936.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(33594..34307)
                     /gene="aphA"
                     /locus_tag="ACHK07_002623"
     CDS             complement(33594..34307)
                     /gene="aphA"
                     /locus_tag="ACHK07_002623"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO8102937.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            34725..>34810
                     /locus_tag="ACHK07_002624"
     CDS             34725..>34810
                     /locus_tag="ACHK07_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102938.1"
                     /translation="MNISYVNSNKTTSLPVELDTLNNKDISYA"
BASE COUNT         8688 a         9242 c         8862 g         8018 t
ORIGIN      
        1 tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta aattccctgc
       61 tctgttagtg gttgcgttgg cgctggttgc ccttgttttc gttatctggc gcgtagacag
      121 tgcgccatca actaatgacg cttacgcgtc agcagatacc attgatgtgg tgccggaagt
      181 cagcggccgc attgtagaac tggcggtcac cgacaaccag gcagtcaaac agggcgattt
      241 gctgttccgc attgacccgc gcccgtacga agccaatctg gcgaaagctg aagcctccct
      301 cgcggcactg gataagcaaa ttatgctcac ccagcgtagc gttgacgcgc aacagtttgg
      361 tgccgactcg gttaacgcca cggtagaaaa agcccgtgcc gccgctaaac aggccacaga
      421 tacattacgc cgcaccgagc cattactgaa agaaggtttt gtctcagcgg aagacgttga
      481 ccgtgcgagg accgcgcagc gcgctgcaga agcggatctt aatgccgtat tgttacaggc
      541 gcagtcagcc gccagcgccg tcagcggcgt cgatgcgctg gtggcgcaac gtgcggcggt
      601 cgaagcggat attgccctga ccaaactgca tctggaaatg gcgaccgttc gcgcaccgtt
      661 tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg ccatgcgccc
      721 tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc gccaacttcc gcgaaaccga
      781 tctgaaaaat attcgctcag gtacacccgc aacgattcgc ctgatgagtg acagcggcaa
      841 aactttcgag ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg acggcggcct
      901 ggtgctgggc ggcctgccaa aagtttctcg ctctattaac tgggtccgcg ttgcccagcg
      961 ttttccggtc aaaatcatgg tcgataaacc tgacccggaa atgttccgca tcggcgcttc
     1021 ggcagtcgct aatcttgagc cgcaataatg agcgcgctca actccctgcc attaccggtg
     1081 gtcaggctgc tggcattctt tcatgaagag ttaagcgagc ggcgaccagg tcgcgtgccg
     1141 cagaccgtgc aactctgggt aggctgcctg ctggtgattc tgatctcgat gacttttgag
     1201 atcccttttg tggcgttatc gctggcagtg ctgttttacg gtgttcagtc gaacgcgttt
     1261 tacaccaaat ttgtcgcgat cttgtttgtg gttgccacgg tgctggagat cggcagcctg
     1321 tttttgatct acaaatggtc atacggcgaa ccgttgatcc gcttgatcat cgccgggccg
     1381 atcctgatgg gctgtatgtt tttgatgcgc acccatcggt tggggctggt ctttttcgcc
     1441 gtcgccattg tcgctattta cgggcaaacc ttccccgcca tgctcgacta tccagaagtg
     1501 gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct atccaacctt gctgatgacg
     1561 ttaatcggcg tactgtggtt tcccagtcgt gccatttcgc aaatgcatca ggtgcttaat
     1621 gatcggcttg atgatgccat tagccacctg acggacagcc tcgcaccgct acccgaaacg
     1681 cggattgaaa gagaggcgct ggcgctgcaa aaactcaatg tcttttgcct cgcggacgat
     1741 gccaactggc gaacccagag tgcatggtgg caaagctgcg tggcaacggt aacctacatt
     1801 tactcgacgc tgaatcgcta cgatcccacc tcttttgctg attctcaggc aattattgaa
     1861 ttccgacaaa aattagcttc agaaatcaac aagctgcagc atgccattac cgaaggtcag
     1921 tgctggcaaa gcgactggcg gatcagtgaa agtgaagcga tggcggcacg ggaatgtaac
     1981 ctggagaata tctgccagac gctgttacag cttggtcaga tggacccgaa tacgccgcca
     2041 acgcccgcag ccaaaccgcc atcaatggcc gccgatgctt ttaccaatcc agactatatg
     2101 cgctacgcgg taaaaacgct gctcgcctgt ttgatctgtc acaccttcta cagcggcgtg
     2161 gactgggaag gcattcacac ctgtatgctg acctgcgtga tcgtcgctaa tccgaatgtc
     2221 ggttcatcgt accagaagat ggtgctgcgt tttggcgggg ccttttgcgg cgcgattctg
     2281 gcgctgttat tcacgctact ggtcatgccc tggctggaca atattgtcga attgctgttt
     2341 gtgctggcac cgattttcct gttgggcgca tggattgcca ccggctctga acgctcttct
     2401 tatatcggca cacagatggt ggtcaccttc gcgctcgcca cgctcgaaaa cgtttttggc
     2461 ccggtgtacg acctggtgga aattcgcgat cgcgccctgg gtatcatcat tggtaccgtg
     2521 gtgtccgcgg tgatttacac ctttgtctgg cctgaaagtg aagcgcgcac gctgccgcaa
     2581 aaactgactg tcgcgctggg tatgttaagt aaagtaatgc ggatcccacg ccagcaggaa
     2641 gtcacggctc tgcgcactta tctgcaaatt cgtataggtc tgcatgcggc gtttaatgcc
     2701 tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac tggacagcga agaacgcgcc
     2761 ttactgattg aacgttcgca aacggttatt cgtcagggcc gcgatcttct tcacgcctgg
     2821 gatgccacct ggaactcggc gcaggcgctg gataacgcac tacagccgga cagagcaggt
     2881 cagtttgccg acgccctgga aaaatacgct gccggtctgg caaccgcact cagccgttct
     2941 cctcaaataa cgcttgaaga gacacccgcc tctcaggcca tcctgcccac cttattaaaa
     3001 caggagcaac acgtctgcca gcttttcgcc cgcttgccag actggacagc cccggcatta
     3061 acacccgcca cggaacaggt acaaggagcc acgcaatgat caatcgtcaa ctttcacgtc
     3121 tgctgttgtg cagcattctc ggcagcacga cgctgatttc cggctgtgcc ctggtacgta
     3181 aggattctgc gcctcatcaa cagctcaaac cggaacaaat caaactggcc gatgatattc
     3241 atcttgccag ctccggctgg ccgcaggcgc agtggtggaa acaactcaat gacccgcagt
     3301 tggatgccct gatccagcgg acgctaagcg gttcacacac cctcgcagaa gcgaaactgc
     3361 gggaagaaaa agcgcagtcc caggccgatt tgttagatgc cggttcacag ttgcaggtgg
     3421 cagcgttagg gatgctcaac cgccaacgtg tctcggcgaa cggcttttta agcccttatg
     3481 cgatggatgc gccagcactg gggatggacg ggccgtacta tacggaagcc acagtaggtt
     3541 tgtttgccgg actggatctt gatttgtggg gtgtgcatcg ctcagcggtt gccgccgcca
     3601 ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt agagctatcg ctgaccacgg
     3661 gcgtagcgca gctttattac agtatgcagg ccagctatca gatgctcgat ctgttagaac
     3721 aaactcgcga tgtgattgat tacgcggtga aagcgcacca aagtaaagtg gcacacggtc
     3781 tggaagcgca agtgcctttc cacggcgcgc gggcacagat tctggcggtc gataaacaaa
     3841 ttgctgccgt taaagggcaa atcaccgaaa cgcgagaatc tctgcgcgca ttgattggcg
     3901 cgggagccag cgatatgccg gagatcaaac cggtggcatt accgcaagtc cagactggca
     3961 ttccggcgac gctctcttat gagttgctcg ccagacgccc ggatctgcaa gccatgcgct
     4021 ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg ggcgctgttc tacccaagct
     4081 ttgatatcaa agcgtttttc ggccttgatg ccatccacct ggatacctta ttcaaaaaaa
     4141 acagtcgcca gttcaacttc atcccgggtc tgaaattgcc gctgtttgac ggtggacggt
     4201 tgaatgccaa tctcgaaggc actcgcgccg ccagcaacat gatgattgaa cgttacaacc
     4261 agtcagtact gaacgcggtg cgtgacgttg ccgtcaacgg cacgcgtctg caaacgctca
     4321 acgacgagcg agaaatgcag gctgaacgcg tggaagcaac gcgcttcacc cagcgcgctg
     4381 ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca ggccactgaa gcccggttgc
     4441 cagtacttgc cgaagagatg tcattactga tgctggacag ccgccgggtg ctccaaagca
     4501 ttcagttgat gaaatcgctg ggcggcgggt atcaggcagg tcccgtcgtc gagaaaaaat
     4561 aaaatgtctg ccgcgtgatg gctgtcacgc ggtatttcgt ttcgtcacgt caaaactgac
     4621 gacagcctgt ttttcgtcag agttttgaat aaatagtgcc cgtaatatca gggaatgacc
     4681 ccaaataaaa tgtggcataa aagatgcata ctgtagtcga gagcgcgtat gcgtgatttg
     4741 attaactgga gcgagaccga tgaaaaaagt cgtcacggtt tgcccctatt gcgcatcagg
     4801 ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc cgggcggagg cagcgcaggg
     4861 gaaaaccaac cagggtaccc tgtgtctgaa gggttattat ggctgggatt tcattaacga
     4921 tacccagatc ctgaccccgc gcctgaaaac ccccatgatc cgtcgccagc gtggcggcaa
     4981 actcgaacct gtttcctggg atgaggcact gaattacgtt gccgagcgcc tgagcgccat
     5041 caaagagaag tacggtccgg atgccatcca gacgaccggc tcctcgcgtg gtacgggtaa
     5101 cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt attggtacca ataacgttga
     5161 ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt ctgcaccaat cggtcggtaa
     5221 tggcgcaatg agcaatgcta ttaacgaaat tgataatacc gatttagtgt tcgttttcgg
     5281 gtacaacccg gcggattccc acccaatcgt ggcgaatcac gtaattaacg ctaaacgtaa
     5341 cggggcgaaa attatcgtct gcgatccgcg caaaattgaa accgcgcgca ttgctgacat
     5401 gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg aatgcgatgg gccatgtcat
     5461 tattgaagaa aatctgtacg acaaagcgtt cgtcgcttca cgtacagaag gctttgaaga
     5521 gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt gaagatatca ccggcgtcag
     5581 cgccagtgag attcgtcagg cggcacggat gtatgcccag gcgaaaagcg ccgccatcct
     5641 gtggggcatg ggtgtaaccc agttctacca gggcgtggaa accgtgcgtt ctctgaccag
     5701 cctcgcgatg ctgaccggta acctcggtaa gccgcatgcg ggtgtaaacc cggttcgtgg
     5761 tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg ccggatacgt atccgggata
     5821 ccagtacgtg aaagatccgg ctaaccgcga gaaattcgcc aaagcctggg gtgtggaaag
     5881 cctgccagcg cataccggct atcgcatcag cgagctgccg caccgcgcag cgcatggcga
     5941 agtgcgtgcc gcgtacatta tgggcgaaga tccgctgcaa actgacgcgg agctgtcggc
     6001 agtacgtaaa gcctttgaag atctggaact ggttatcgtt caggacatct ttatgaccaa
     6061 aaccgcgtcg gcggcggatg ttattttacc gtcaacgtcg tggggcgagc atgaaggcgt
     6121 gtttactgcg gctgaccgtg gcttccagcg tttcttcaag gcggttgaac cgaaatggga
     6181 tctgaaaacg gactggcaaa tcatcagtga aatcgccacc cgtatgggtt atccgatgca
     6241 ctacaacaac acccaggaga tctgggatga gttgcgtcat ctgtgcccgg atttctacgg
     6301 tgcgacttac gagaaaatgg gcgaactggg cttcattcag tggccttgcc gcgatacttc
     6361 agatgccgat caggggactt cttatctgtt taaagagaag tttgataccc cgaacggtct
     6421 ggcgcagttc ttcacctgcg actgggtagc gccaatcgac aaactcaccg acgagtaccc
     6481 gatggtactg tcaacggtgc gtgaagttgg tcactactct tgccgttcga tgaccggtaa
     6541 ctgtgcggca ctggcggcgc tggctgatga acctggctac gcacaaatca ataccgaaga
     6601 cgccaaacgt ctgggtattg aagatgaggc attggtttgg gtgcactcgc gtaaaggcaa
     6661 aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa ggggcgattt acatgaccta
     6721 ccagtggtgg attggtgcct gtaacgagct ggttaccgaa aacttaagcc cgattacgaa
     6781 aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg atcgccgatc agcgcgccgc
     6841 cgagcagtac gtgattgacg agtacaacaa gttgaaaact cgcctgcgcg aagcggcact
     6901 ggcgtaatac agtcctttct acagcctcct ttcggaggct gtttttttat ccattcgaac
     6961 tctttatact ggttactccc tacccaatcg tattatcaaa atgaaaaaaa ttatcgcatt
     7021 gatgttgttt ttgacattct ttgcccacgc gaacgactcc gagcctggca gccagtattt
     7081 aaaggcagca gaggccgggg accgacgcgc acaatatttt cttgccgaca gctggtttag
     7141 ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa gccgccgaca gcggtgatgc
     7201 tgatgcctgc gcgctgctgg cgcagatcaa aatcaccaat ccggtcagtc tggactatcc
     7261 acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc agtaaagaag gtgaagtaac
     7321 gctggcgcat attctggtaa atactcaagc gggcaaaccg gattatccaa aggcgatttc
     7381 gctgttagaa aacgcctcgg aagatctgga gaacgactct gccgtcgatg cccaaatgct
     7441 gcttggtttg atttacgcca acggcgtggg cattaaggcc gacgatgaca aggcaacctg
     7501 gtatttcaaa cgcagctctg caatttcccg aaccggttat tccgagtact gggcgggaat
     7561 gatgttctta aacggtgaag aaggttttat cgagaagaac aagcaaaagg cgctgcactg
     7621 gttgaacctg agctgtatgg aagggtttga taccgggtgt gaagagtttg aaaaattaac
     7681 caacggttaa ggtaggcctg ataaggcgcg ttaagcgtcg catcaggcgt tgatgtcgga
     7741 tgcggcgtaa acgccttatc cgacctacaa aatcacgcta aatcaggcta ttgcgctgtc
     7801 tggcgtaggc ctgataagac gcggcaagcg tcgcatcagg cattgatgtc ggatgcggcg
     7861 taaacgcctt atccgaccta caaaatcacg ctaaatcagg ctattgcgct gtctggcgta
     7921 ggcctgataa gacgcggcaa gcgtcgcatc aggcattcac acattgccgg ggggatatcc
     7981 ccggcaatct tcaattattg atccgcagtt ttatcaaatt tgcccagcac ttcacgctca
     8041 taagccagcg cctttttgcg gtcaaatttg tgttcccact tggcaatcac cagcaccgcc
     8101 agcgcattac ccaccacgtt cagcgcagta cgcgccatgt cgaggatacg gtcaacacca
     8161 gcaataaacg ccagaccttc cagcggaata cctacgctac ccagcgttgc cagcaacacc
     8221 acaaacgaca cgcccggcac gccagcaatc cctttcgagg tcaccatcag cgtcagcacc
     8281 agaatgattt cctgccagat ggacaggtca atgccataca gctgagcgat gaaaatagcg
     8341 gcaatacttt gatacagcgt cgaaccatca aggttaaaag agtaaccggt cggcaccacg
     8401 aagctggtga tcgacgccgg tgctccgtag gcttccatct tctcaataat tcgcggcagt
     8461 acgctttcag agctggcagt ggagtacgcc agaatcagct catctttcag aatacgaatc
     8521 aggatccaga cgcttaaccc gcacaggcgc gccacaattc ccagcactac cagcgcgaag
     8581 aacagaatgg cgaaatgcac cagcagcacc agtttcgcca gtggccacag agaagagaaa
     8641 ccaaagttag ccaccgtcac cgcaatcagc gcaaacacac ccaccggcgc ataacgcatc
     8701 accatgtgag tcactttaaa catggtttca gagatggagc ggaacacggt caccagcggt
     8761 tcacgatgcg tcgcgggcag ggaggaaagc cccagaccaa acagcaccga gaagaagatg
     8821 atcggcagca tttcgccttt cgccatcgac gccacaatat tcgtcggcac cagcgacaaa
     8881 atcgtgccca taatgccgtg ggaactgctt tgtaccgctt ccgtagtgct ctgatatttc
     8941 gagatatcga cggtcgccaa ctgcgacata tccaccccgg caccgggctg gaagacgttc
     9001 gccagagtga tccccaaaat gatggcgacg gtggtgatca cctcgaagta gataatggtt
     9061 ttcgcgccaa tacgcccgag ctgtttggca tcaccaacac ccgcgatacc caccaccagc
     9121 gtggagatca caatcggcac aacaatcatt ttgatcagat ggatgaagat atcacccgcc
     9181 ggagagagca aattgacgac cagccagtcg cggctgtcgc tatggtagtg caggtagctt
     9241 cccaggagaa tgcccagcac catagcaaac agaatctgcc aggccaggct gaattttata
     9301 tttttcatca gaataacttc ctcaatggag cgccgttccc cgttcagaaa cgcgagaacg
     9361 tgacatactg ccagggcatg aattgtgttg cgttggtttg tagatttttt taacgcttat
     9421 gatcagtatc atttgcgcgg tatgttgcgc aaccctttaa caacagagct tttcgctgca
     9481 ttaatgcata ataacgcgag tttatggcag gattaatgaa taaccatttg ttatggaaag
     9541 atttttattt gtttaatctg caataatact gattggtgat tatttttggt atatgttcat
     9601 tcgattattt tttatgcagc tcaataacat aatcttcatc accagatgat taatccgatc
     9661 tccgctgcaa caatttcgcc atattaatcg actcgaccag ctgtgtccgg ttgatccggg
     9721 gtgagttgag atccatcact ttttgccata gttcgatggc ttgcgcgtaa ttcgcctgca
     9781 taaacgcatc ggaagccagc agcatcagag cggtgatttc gttagagtcc agcgcgaggg
     9841 ctttgtcgat cattgcgcga gtctgggcgg tcatatgctg gctggcctgg taataaagca
     9901 ccgtcgccag cgccgcatac agttcagcgt tctcaccacg tagttgcaac gcctgacggt
     9961 acgccagcag cgaattgctg taatcgtttt gccacagata gtactcgccc agtaacgccc
    10021 actgttcgct gttttgcgga ttagcgcgga ttttatcctg caatgcctga agctgcgctt
    10081 ccggggtttg ctggctggca aactgatgta gcggatcgcg ctgacgctga tactccgcac
    10141 gtaccgcctg ccattttggg cttaacagat aactgccgac acagaggaat accatcaata
    10201 tcgtgagtgt tgtcagcatt ttcaccggta tttgaggctg tttcatcgct tcgccctcac
    10261 tcgccagagg atcagtgcca tcagcagtaa caacaccact ggcagcgccc acaacaccag
    10321 cgtctgaccc gttaacggtg ggttatagcg aacaaaatct ccgtagcgtt cggtcatcca
    10381 gccaatgatt tcgacttcac ttttcccctc cgccaccatg ctgtaaacct gatggcgcat
    10441 actgactgcc accggcgcgt tggattccag taagttttga ttctggcact gcggacaacg
    10501 taactggctg gcaatattta acgcctgttg ctgttgttgc ggattggcga attgccaggt
    10561 gtctacaacc tgagcgcgcg caaaacaggt aaataacagc agtagcgtga gaagtccttt
    10621 attcatcgcg cttcctcccc cgccatccgc ttaacaatgc gcccgcaatc atcaacaatc
    10681 ctccccccca gatccagcgc acaccgcttt gtacatacaa acgaaaagcg taacgatccg
    10741 gcccagtttt ctcccccatg accgcatacc agtcatggat gccgttccag cgaattgacg
    10801 gttccatcat ttgctgacgg cgtgcttcat aaaaacgccg ctccggcgtc agttcaccaa
    10861 tacgttgctg atggtcaaac agtgccacta tcgctttttc gctggtgtaa ttgcctctgg
    10921 cttgcagatc gaggcgctca aaacggaagg tgtatcctgc cagcgtcacc tgctgacccg
    10981 gctgtaaatt caggctgatt tcctgacggc tgacgctgga aaccacgacc ccagcggcaa
    11041 ataacagcac gcccgcatga gctaccagcg ccggaagctg cacgcgtttg ccagagacaa
    11101 acgtcgccag cacaatcacc accaacatca acagaccaaa cggtaacgtc gcgcggttaa
    11161 aatacggtgc gccaacggag aggcgtcccc agcccagcag gccgtaaatc atcggataaa
    11221 gcgttcccac cagcacgatc agtagcactg cgcaaaacag caacagcgta gcgaggatta
    11281 acatttcccg cgataacccc gaaaaacgca ccgccgggcc accgtcccgc gctcgccagc
    11341 catacagagc cagagacgca aggctaatca gtacaaacag actgaacaac ggcacggcgc
    11401 ggacgttatc cagcgcgaac gcatgaaccg aaaccagaat gccagaacgg acaattaagg
    11461 tgcccagcag cgacagcatc agagtaacta tcgccagtaa cagcgaccag tggcgaaaaa
    11521 tcccccgctg gcgtgtcagg gataaactgt gcagcaacgc agtggcagaa agccagggta
    11581 gtaaagaggc gttttccacc ggatcccaga accaccagcc gccccagccc agttcgcaat
    11641 aggcccacca ggaaccgagg atgatccccg cagttaatgc actccagcca ggtaacgccc
    11701 agcgccagca aattcgggcg caggcaccat caaactcgcc gcgcagtaga ctcgccagcg
    11761 ccacgctcgc cgctaccatc aaaccgccat agccaaggta aagcagcggt ggatgaaaga
    11821 taagaccggg atgttgcagc atcggattga gatcgcggcc ttcgattgct ggtggaaata
    11881 tccgcacgaa gggatcggac cacagcacta caaacagtag cagtgcggcg agcatgagag
    11941 ataaaacggc taacgtcagc ggaaatagcg gatcggtttg ctgccgataa tgccaggcaa
    12001 acagcgcgct ccaggcggaa agcagcagca cccaaagcag cagcgaacct tcatgaccgc
    12061 cccacaccgc cgccagtttg agttcccacg acagcaggct gtagctatgt tgggcgacat
    12121 aaatgaccga gaaatcgctg atgagaaaac aatacgtcag tacgccaaag gcgagcagca
    12181 ggagcgcgaa ctgtgccaga atgccgatgc aagtgagtcg catcatggca ggccagcgca
    12241 accgcactcc cgcaaaggcc gtcaacgggg tcaacacgtt gaccccgaga cttaacaaca
    12301 gcgccagaaa accgacttca ggaaggaata cgtccagaaa ccacctctgt tctggcttac
    12361 gccaccgtta actgcccggc gtagagaatg aaaaagcgca acatcaacac gccgaccagg
    12421 ctggctccac acgccgccag cacggcagga atgccggaac tgcgattgac ccacggtttg
    12481 agcaacattg gcacaatcag ccccagcccg gcgacaccaa gccagaacca ccacgtccag
    12541 aaaccgccac ccaatgccgc caccagcgca cgcactttac cgtcatcgcc caaggccaga
    12601 ccgacaaaaa acgccaccag caggaagatt tcaccccata ccacggggat ttccatacgg
    12661 tgtacaaact gcgcttccgt ggaatgcggg ttactgcgtt ggcgtatcgc catggcgatc
    12721 agtgccaccg ccgcaccgga cgagatgccg gagaagagga acagcaccgg caggatcggg
    12781 ttattaagga acggatacga tttcagcgcc gacagcagaa aaccggtata agcccccaac
    12841 aacaccgcca gcaccagcat caatgtttcc agtccgcgat gaacgggcgt cagcaggctc
    12901 agaacctttt gcacgatccc cagcttcggc aaccagcgtt gttgcagggc aagcaaatca
    12961 tgttcaaaga ttttcgccag ccacagcacc agcaccacca tgtaaagctg aaacagcatc
    13021 acccccatcg acatcaccga ggtaaaactg tagtggaaca tcagcttcca gaaggtccac
    13081 ggtcttgtca ggtggaagac gaggatcaac agaccgagga tcaccgcgcc aggtccgaca
    13141 atcagcgtgg tgcgcagcaa cgtgctgtct gcaccgcccg cctgcgggta gaagcgacgt
    13201 aacagcacag ccagcgtcac caaaccagca gaaataccaa tcaaaaacag gtagatggca
    13261 atcggccagt cccacaccag cgattcaaaa tgaaatgcgg aagtctgcgt cattggctca
    13321 cctccccgta tttaaacggt acgcggtaca gtttcggttt ggttcccagc gccagcttgt
    13381 agcggtaagt aggcttctgg cgcagcagtt gcgaaatctc actgttagga tcgtccagat
    13441 tgccaaacgt cagcgccttc gtcgggcaag cctcaacgca cgcgggcagc ttaccggcct
    13501 gcaaattggt cttacggcag aaatcgcatt tgtccgccgt cttcgtgacc ggatggataa
    13561 agcgcacgcg gtacggacag gcggcgatgc agtactgaca accgacgcag agatcaggat
    13621 taacatcgac aatcccgcta gcggcatcac gaaaagacgc accggtcggg cagacgtcaa
    13681 cgcacggcgc atgatcgcag tgctggcaag acttacggaa gaagcgatat ttcacatcag
    13741 gaaattcgcc ctgcggctcg ctacgaataa tcgtcaggcg cgagacgcct tccggcacct
    13801 tgttcacttc ccgacaagca tccatacagg cggtacagcc gatgcataac gactcgtcat
    13861 gcaccatgcc gtaacgcacc ccattgatat tcaacgtttt cgccaccacg cgccctgcgg
    13921 tgccgctgac ggctgccagc acgccgacgc cggtgagaaa ctggcgacga gaccaggtca
    13981 tggctgctcc ttaagcaacg gaacagacgc cgggttaaag ttcggattgg tgcgctgatc
    14041 gctgtggcaa tcgacgcaaa tcttaatccg tcctttgtca cttaacgtct gcatcgtatc
    14101 ttgctgtgga tgcagggaat ggcagctggc gcacgccact ttggttacgt ggacatcgtg
    14161 cggccagaac gctttttgca actgttccgg cagatggcag gacatacaga cgctgttctg
    14221 ctctccaacc ttgtacatcg gctcgttaaa gcgcatcaca tctttcaccc cttcgcggtg
    14281 ttgcggtgat ggctggccgt gacagttggt gcaggtgacc ggcagtttgt tattcgggtt
    14341 gataacggaa gcatgtttgc catgcatacc ttcggtatct ggcttatgac agtccagaca
    14401 ggcggcatcc gggttacgct gctgggtaac ttcgtagcga tcatcagatg cctgcgccgc
    14461 aggggtagcg gtaattccgt tcaggctcca caacaggccc gacgccagca ccccggcagt
    14521 taataacgaa cgtaatacgc tcatattcac tccattgtgt tttccgcccc ttgcgaggcg
    14581 gaacggggtt attggcttaa cagaccgttt ttacgtgcct gctcttccca ctgcgggatc
    14641 accgttttga tgaagtcctg cttctcggcc ttgatttgtt ccatgttcag gccaatggcc
    14701 tgctgggctt tctctttggt cgagatatcc gggatctgga tttcatgggt gatgcctttg
    14761 gtcgccagca ggcgcgccag tttggtgcgt gcatccgccg ctttatccat cgccgtaccg
    14821 agcatccgta aaccttcttc cggtgcgtgc atatgaatgc cgtgggaagc gatcgccaga
    14881 tcccagcgcc actgggcatg acggatatcg tcctgaattg gcttcatttc ggcttccgtc
    14941 gcgcctgcat ccagcgctgc tttcgcttcg aagtgagcgt gaaccagttg atcttcaacc
    15001 ttgattttca ggtcgttaat cgactgctta cgttccgcga ccactttttg caaggcagct
    15061 ttgtcctggg tatggcagtt cgcacaagtc tgggcgaagt tatcaaacgg attaccaatt
    15121 ttatggtcgg tgtagagttt gccttcggcg ttctgcactt ttggcatatg gcagtcgata
    15181 caggtcacgt tgtttttacc gtgaatgccc gctgtccagg tttcatattc cgggtgctgc
    15241 gctttcagca ttggcgtttt cgacagggag ttagtccagt cagagaaggc aattttgtcg
    15301 taatactgct ccatattttc gactttcatg ccgtcatccc acgggaattt aaccgctttg
    15361 tttttgccgt cgaagtaata ctccacatgg cactgaccgc aaaccatcga ttgctggtcg
    15421 aaacgtccgg ctttctcaaa aggtttacca atggcttcca tcgcgcgagc cgcatacgga
    15481 cgggaaaggg ttaactccgg tttgcctttg gcgaactctg gtgaggcggt gttatggcaa
    15541 tcggcacaac ctaagttgtt gacaatttcc gggccgccgc gcgcccattt accgtggaag
    15601 tagccatctt cgccgtcttt ctggatcaga cgcgccacat ccgggctttt acaactccag
    15661 cacgccatcg gtagcggacc atcttcagcg tttttcggcg caccggtacg cagggtttca
    15721 cgcacatcgg tcacagcaaa agcatgtcca cgcggcttgt tgtaatcgcg cgagaaggga
    15781 taccccgccc acaggatcac cagccgtgga tcttccgcca gggcgtcaac acgctctgac
    15841 tgttccgagg tggctttcca ggagagatat tgatcgggat gctgcggggc aaaggtttca
    15901 ttcttcgctt ccacagttac aggttttgcg ggagcagccg tttgttcagc gtgaacagaa
    15961 gtgaaaaaga aaaaaggaat caataagctg aagatacggc gtgcgtttat ttttatcctt
    16021 gtcatagggg cttcatccga attgcgccat tgttgcaatg gcggttttta ttgtttttca
    16081 cgacagtaac cgcacctaca ctgtcatgac attgctcgcc cctatgtgta acaaataacc
    16141 acactgtgaa tgttgtcttt aatcaattgt aagtgcatgt aaaataccac tttagagtta
    16201 gtcagtatct tcctcttttt caacagcatg cataactgca tgttcctcaa agaattaatc
    16261 aacttttgtt gctgaccttc aaaaattacc ctgccgttta tttgcacaat tctacttttg
    16321 cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac ccctacattt aacgcttatg
    16381 ccacatatta ttaacatcct acaaggagaa caaaagcatg agtcaaattc acaaacacac
    16441 cattcctgcc aacatcgcag accgttgcct gataaaccct cagcagtacg aggcgatgta
    16501 tcaacaatct attaacgcac ctgatacctt ctggggcgaa cagggaaaaa ttctcgactg
    16561 gatcaaaccg taccagaagg tgaaaaacac ctcctttgcc cccggtaatg tgtccattaa
    16621 atggtacgag gacggcacgc tgaatctggc ggcaaactgc cttgaccgcc atctgcaaga
    16681 aaacggcgat cgtaccgcca tcatctggga aggcgacgac gccagccaga gcaaacatat
    16741 cagctataaa gagctgcacc gcgacgtctg ccgcttcgct aataccctgc tcgagctggg
    16801 cattaaaaaa ggtgatgtgg tggcgattta tatgccgatg gtgccggaag ccgcggttgc
    16861 gatgctggcc tgcgcccgta ttggcgcggt gcattcggta attttcggtg gcttctcgcc
    16921 ggaagcggtt gccgggcgca ttatcgattc caactcacga ctggtgatca cttccgacga
    16981 aggcgtgcgc gccgggcgta gtattccgct gaagaaaaac gttgatgacg cactaaaaaa
    17041 cccgaacgtc accagcgtag agcatgtggt ggtactgaag cgtactggcg gaaaaattga
    17101 ctggcaggaa gggcgcgacc tgtggtggca cgacctggtt gagcaagcca gcgatcagca
    17161 ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt ctctatacct ccggttctac
    17221 cggaaaacca aaaggcgtac tgcacactac cggcggttat ctggtgtacg cggcgctgac
    17281 ctttaaatat gtctttgatt atcatccggg cgatatctac tggtgcaccg ccgatgtggg
    17341 ttgggttacc ggacacagtt atttgctgta cggcccgctg gcctgcggcg cgaccacgct
    17401 gatgtttgaa ggcgtaccga actggccgac gcctgcccgt atggcacagg tggtggacaa
    17461 gcatcaggtc aatattctct ataccgcgcc cacggcgatt cgcgcgctga tggcggaagg
    17521 cgataaagcg atcgaaggca ccgaccgttc gtcgctgcgc attctcggtt ccgtgggcga
    17581 gccaattaac ccggaagcgt gggagtggta ctggaaaaaa atcggcaacg agaaatgtcc
    17641 ggtggtcgat acctggtggc agaccgaaac cggcggtttc atgatcaccc cgctgcctgg
    17701 cgctaccgag ctgaaagccg gttcggcaac acgtccgttc ttcggcgtgc aaccggcgct
    17761 ggtcgataac gaaggtaacc cgctggaagg ggccaccgaa ggcagtctgg tgatcaccga
    17821 ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac gaacgttttg agcagaccta
    17881 tttttccacc ttcaaaaata tgtatttcag cggcgacggc gcgcgtcgcg atgaagatgg
    17941 ctattactgg ataaccgggc gcgtggacga cgtgctgaac gtctctggtc accgtctggg
    18001 gacggcggag atagagtcgg cgctggtggc acatccgaag attgccgaag ccgccgtagt
    18061 aggtattccg cacaatatta aaggtcaggc gatctacgcc tacgtcacgc ttaatcacgg
    18121 ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac tgggtgcgta aagagattgg
    18181 cccgctggcg acgccagacg tgctgcactg gaccgactcc ctgcctaaaa cccgctccgg
    18241 caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc gataccagca acctgggcga
    18301 tacctcgacg cttgccgatc ctggcgtagt cgagaagctg cttgaagaga agcaggctat
    18361 cgcgatgcca tcgtaaccca caattgccgg atgcggcgta taacgcctta tccggcctac
    18421 attcggcaag ggttacccga gcgttaacct tctcccataa gggagcggga attaaaacaa
    18481 tccctacatt acctctggag aatctgtgat gaatggtacg atttatcagc ggatagaaga
    18541 caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg tttgccacca tcctgtcgat
    18601 tattatgctg gcagtttata tcggctttat tttactgatc gccttcgcgc ccggctggct
    18661 gggcaccccg ctgaatccga acaccagcgt cacacgcggt attccagtcg gtgttggggt
    18721 aatagtgatc tcctttgttc tcactggtat ctacatctgg cgggcgaacg gcgaattcga
    18781 ccgtcttaat aacgaagtcc tgcatgaggt acaagcatca tgaaaagagt tctgacggcg
    18841 cttgccgcca cactcccttt cgcagctaac gccgcggatg ctattagcgg ggccgtagag
    18901 cgccagccaa cgaactggca ggcgattatt atgttcctga ttttcgtcgt gtttacgctc
    18961 ggcattacct actgggcgtc aaaacgcgta cgttcgcgta atgactatta caccgcaggc
    19021 ggcaatatca ccggttttca gaacgggctg gcgattgccg gtgactatat gtccgccgcc
    19081 tcatttttgg ggatctccgc actggtgttt acctccggct atgacggctt aatttactcg
    19141 ctgggcttcc tggtgggctg gccgatcatt ttgttcctga ttgccgaacg tctgcgtaac
    19201 ctggggcgtt acacctttgc cgatgtagcc tcttatcgtc tgaaacaagg gccgattcgt
    19261 attctttcgg cctgtggttc tctggtggtg gtggcgcttt accttatcgc ccagatggtg
    19321 ggcgcaggta aactgatcga gctgctgttt ggccttaact atcacattgc agtggtgctg
    19381 gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca tgctggcgac cacttgggta
    19441 caaattatca aagctgtgct gttgctgttc ggtgccagtt ttatggcctt tatggtgatg
    19501 aaacacgtcg gctttagctt caacaatctg ttcagcgaag cgatggcggt acacccgaaa
    19561 ggtgtcgaca tcatgaagcc gggagggctg gtgaaagatc cgatctccgc gctgtcgctg
    19621 gggcttggat tgatgtttgg tacggctggc ttgccgcaca ttctgatgcg cttctttacg
    19681 gtcagcgatg cccgcgaagc gcgtaagagc gtgttctacg ctaccggatt tatgggctac
    19741 ttctatatcc tgacctttat tatcggcttc ggcgcgatca tgctggtcgg tgcaaatccg
    19801 gaatataaag acgcggcggg ccatctgatt ggtggtaaca acatggcggc cgttcacctg
    19861 gcgaatgcag tgggcggcaa cctgttcctc ggttttattt cagcggttgc tttcgccact
    19921 atcctcgcgg tggttgcggg tctgacgctg gcgggcgcat cagcggtttc gcatgacctg
    19981 tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag agctgcgggt atcgaaaatc
    20041 accgtcctga ttttgggtgt gattgcgatt atcctcggcg tgctgtttga gaatcagaac
    20101 atcgccttta tggtggggct ggcgtttgcc atcgcggcga gctgtaactt cccgatcatt
    20161 ctgctttcta tgtactggtc gaaactgact acgcgtggcg caatgatggg ctgctggctg
    20221 gggctaatta ccgcagtggt actgatgatc ctcggcccga cgatttgggt acagatcctc
    20281 ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc tgttctctat cagcgtggca
    20341 ttcctcggca tctggttctt ctcggcaacc gataactcag cggaaggtgc gcgcgagcgt
    20401 gaactgttcc gtgcgcagtt tatccgctcc cagaccggct ttggcgttga gcaaggccgc
    20461 gcgcattaat ctctcccctt ccccggtcgt ctgaccgggg aatactcttc ctctcccaaa
    20521 tgcatcacct tttcccataa tattaaacaa ataaactcat taaaaaatga gcgatttttg
    20581 acagtcgtag aaaatgataa tgcagagaat atgccttttc tttcttgtta attataagga
    20641 tattttatgc gctataatgg tttaaataat atgtttttcc ctctttgcct gattaacgat
    20701 aaccactctg tcacaagtcc atcacataca aagaaaacaa aatcagataa ttacagcaaa
    20761 catcataaaa acacgttaat tgacaataaa gccctctctc ttttcaaaat ggatgatcat
    20821 gaaaaagtga taggcttgat tcagaaaatg aaaagaattt atgatagttt accatcagga
    20881 aaaatcacga aagaaacgga caggaaaata cataaatatt ttatagatat agcttcatat
    20941 gcaaataata aatgtgacga tagaattacg agaagagttt accttaataa agataaggaa
    21001 gtgtcaatta aggtggtata ttttataaat aatgtcaccg tccataataa tactatcgaa
    21061 atcccacaga cagtaaatgg tggttacgat ttttcacacc ttagcctgaa aggtatcgtg
    21121 attaaagatg aagatttatc caattcgaat tttgcaggtt gcagactaca aaacgctatt
    21181 ttccaggact gtaatatgta taaaacgaat tttaatttcg ccataatgga aaaaatactt
    21241 tttgataatt gtattctcga tgactcatat ttcgctcaga taaaaatgac tgacggaact
    21301 ctaaattcat gctccgctat gcatgttcaa ttctacaatg caacaatgaa tagagccaat
    21361 attaaaaata ccttccttga ttattcaaat ttttatatgg catacatggc tgaggtaaat
    21421 ctttataaag taatagcgcc atatattaat ttatttagag ccgaccttag cttctctaaa
    21481 cttgatttaa ttaactttga acatgctgat ctgtctcgtg tcaacctgaa taaagcaacc
    21541 ctccagaata taaacttaat tgatagcaaa ctctttttta cgcggttaac aaatacgttc
    21601 ctcgaaatgg ttatatgtac cggctctaat atggctaatg ttaattttaa taatgccaat
    21661 ttaagcaatt gccatttcaa ctgttctatt ttaacaaaag cctgtatgtt taatacccgt
    21721 ctctatcggg ttaatttcga tgaggctagc gtccagggaa tgggcatttc cattctccgt
    21781 ggggaggaaa atatccccat tgatagtgat accctggtaa cactacagaa attctttgaa
    21841 gaagattgta cctctcatac tggcatgtca caaactgaag ataatattaa tgcagtcgct
    21901 atgaagatta ctgcagatat tatgcaagat gcagattgag gttaagccga agaacagcca
    21961 atgaattgac tttacaagag agataactta tttgttttag aatcatgaat gttagcccga
    22021 gctaacctgt taaaggtgca aaagattgca cctttttcag attacctcgg gcctggttac
    22081 gtcgctttcg gcttactgat tttcctcaat caacaacgct tcaagcaaat cgagatcgtg
    22141 cagtaatttt tgcagcgttt cgttactgat ctcccgcgtg gcgcgtaggt ggtacagttc
    22201 agcacgttca gaacgcaatg ccgccagacg gaagcgacgc tcaaggttct cttcctgcac
    22261 ggaactttca acgtcgttac gtccatcggc gcgacgacgc aggttaccaa tgacgcgaga
    22321 actgacctcc gtaagcagct ggttatcgat attctcttcg gtatccgccg ccagacgctc
    22381 ctccattttc tggatcgcca caatcgccac ttctgccgtt gccgctcgcg caatacgttc
    22441 ttctttcaat tgttgcgaat ggtccgcgac ttcaatgtgt tgtagcagaa taggcaacat
    22501 caccacgccg acaaacagcg agaagagaat gacaccagcc gccagaaaca ccagctcata
    22561 gcgcgccggg aagacgttac catccggcaa gagcagcggg atagagagca caccggccaa
    22621 agtgatcgcc ccacgcaccc cggcgaaaga cgcgatcagg atttctcgtg tggtccacga
    22681 accaaactcc atcggcttct tcttcaggaa gcggttgctg aactttttca tcgtccacag
    22741 ccagccgaaa cggaccagca tcagcgccgc atatatcaga ataatatcgg taaacagcat
    22801 ccagatttcg acgttagggt cgatttctgc tgccatcagc gacgtctcca gaatacccgg
    22861 cagctgcaga cctaacagca ggaataccat gccgttaaag acaaattcca gcatcgccca
    22921 ggtgctgttt gcacgcaggc gcattgccag cggcgcacgg cgcatcacac cggagcgggt
    22981 gatggtcatc ccggcggcaa ccgcagcgag gatgccggaa acgccaatat gttccgcaat
    23041 cagataagaa gcgaatggca gcaggaacaa caggacgatc tgcgtcgcgg gttcatcacc
    23101 gccccagcgg ctgaggaatc gcagcgagcg accgtacagc cagctaacca caaaaccggc
    23161 gagaataccg ccaatggcta ctttcataaa ttcgaccgtc gcaccgccga cggtgaagat
    23221 catcgtcccc atcgccactg ctacggcaaa cttcaacgac accaggccgg aagcgtcatt
    23281 catcaacgct tcgccctgca aaatgcccat gatttttttc gggatgcgcc cttcgccaac
    23341 aatcccggag agcgccacag catcggtcgg agaaagtacc gccgccagcg caaaggcggg
    23401 gatcagcgga atgcccggca ccacccagta aataaggaag ccaatgccga ccacggtgac
    23461 caccaccagc gccagtgcga ggccgaaaat ctctcgacca tgttcgagaa attcacgggt
    23521 cggcgttttc cagccatcag cgaacagcaa cggcgggata aacaagacta aaaagagttc
    23581 aggatcaaac tccacatgca aaccaaacgt cggccacgcc agtagcgcac cgatggcgat
    23641 ttgcataagc ggaagcggga tctgaaaggg catgacacga gtgaccaccc cggacagcga
    23701 gaccacgagg gtcattatca gtatggtgaa gaagatttcc atgcgttccc tgtttgcgat
    23761 gttttttatg atctgagaaa gacgttatgc ctgaattctc attttttcag aatatcgcaa
    23821 aagctcacac aatgtgcgga gaaaataaaa acgggcagcc attgccaccc gttttcagaa
    23881 tccgatgaaa gattagatag cccagccacc cgcatagaag gtcaccagcg ccacggcgat
    23941 aacgaccgta ccgatgttca acttgcgcca ttcgccggaa accagacgac caatcaccag
    24001 ggtcgcgaag ccgatcatga tgcctgttac gatgttacag gtcagcacga tgaataccgc
    24061 cgtaaccaga cccgccatcg catcaacaaa atcagcaaag tcgattttcg ccacgttgct
    24121 cagcattagc aggccaacgt acatcagcgc cggagccgtt gcataccccg gaacgaggta
    24181 agaaagcgga gagaggaaca gaatcagcag gaacagcacg ccaacggtga tagccgtcaa
    24241 cccggtttta ccgcccgccg ccgtacccgc cgcagactcg atgtataccg ctgccggagc
    24301 tgcacccacc aggccagaga aaacgctgct catggagtca gtggtcagtg ctttcccgcc
    24361 gtcgatgatc tgcccatctt tatccagcag gttcgcctgg ccggcgacgg cacggatagt
    24421 tccggtggca tcaaatactg ccgtcatcac cagcgccaga acgcttggca ggactacagg
    24481 attcagcgcg cccataatgt ccaggctgcc aatcagtgaa ttgccgtttt catcgctcaa
    24541 tgaaggcatg gcgaaaacgc cggagaaatg gacgttagga tcgaagatca aaccgacaat
    24601 tgagataccg ataatggtca gcagaatgcc accagggact ttcagttttt ccaggccgat
    24661 gatcaccgcc agacctacca gtgacataat caccgggaag gtcgcgaaat cacccagcgc
    24721 aacgggcaga ccatcaagcg ggtttttaat caccagaccg acaccgttag cggcaatgag
    24781 cagcaggaac agaccgatac cgatccccgt gccgtgcgcc acaccgtgcg gcaagttgcg
    24841 caaaatccag ctacggatac ccgtggcaga aattaccgta aacagcacac ccatcaggaa
    24901 cacggcaccc agcgcgacag gtacgctaat atgttgcccc agcaccaggc tgaatgcggt
    24961 aaacgccgtc agggagatgg cgcaaccaat cgccaacggc agattagccc acagacccat
    25021 cacgatagaa ccgagtccgg caaccagaca ggtcgcaacg aaaactgccg caggcgggaa
    25081 gcccgcttta cccaacatgc ctggaacgac gataaccgag tagaccatcg ccagaaacgt
    25141 tgttaacccg gcaaccactt cctgacggac agtgcttcca cgttgtgaaa ttttaaacca
    25201 ggcgtcgagt gaaccgccgg tacgcgctga tggcgtagac ataagagaac atcccctgag
    25261 agtttaattt tcgtcaagat gcgtggcgga caatcccctg ccagcgaaac gttatcttcc
    25321 tgctccaggt ttgcgacaat gtgctgcgtc gcaaaaaagc aaacgtttag ctccgcgcat
    25381 acaaaacggg tggtaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc
    25441 gaagtttgct gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc
    25501 gcaaacgtta tcgtcgatgc gcaagattgc agcgttatcg agccgtatac cgttctggat
    25561 tgcgataaaa aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac
    25621 tggcgaagaa acgccggtga catacaatta aagcatcaac accaaccgga acctccacca
    25681 cgtgctcgaa tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgtacca gcgggataga
    25741 gagaaagaca aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgttca
    25801 tcttccagta agcgtgcgcc ggtaccttct tctcctaagc ggtcgcccgg gttacgcaac
    25861 gggcaatcac tgcgcgaaag gcagccacaa ccaatacatc catccagttc gtcacgcagc
    25921 gccactaagg tatgaatgcg ccgatccaac tcttctcgcc attgggacga aagctgtttc
    25981 cactctttcg cacttaacgt atgcccttcg ggcaacacgc caaacgcttc accaatggtc
    26041 gccagcggaa tgccaatacg ctgagcaatt ttgataattg cgacatatcg caacacatca
    26101 cgtttatatc ggcgctgatt gccgctatta cggacactgg taatcaaccc tttgctttca
    26161 tagaaatgca gcgccgatac cgccacaccg ctgcgtttcg ccacttcgcc gggggttagc
    26221 agcgctttaa tgcggggtaa tttcttttcc ataaatcgct ttacctcaag ttaacttgag
    26281 gaattatact ccccaacaga tgaattaacg aactgaacac tgaaaagagg cagatttatg
    26341 tcccatcaga aaattattca ggatcttatc gcatggattg acgagcatat tgaccagccg
    26401 cttaacattg atgtcgtcgc aaaaaaatca ggctattcaa agtggtactt acagcggatg
    26461 ttccgcacgg tgacgcatca aacgcttggc gattacattc gccaacgccg cctgttactg
    26521 gccgccgttg agttgcgcac caccgagcgt ccgatttttg atatcgcaat ggacctgggt
    26581 tatgtctcgc agcagacctt ctcccgcgtt ttccgtcggc agtttgatcg cactcccagc
    26641 gattatcgcc accgcctgta gttttattgc ccgcgcgtta actcccgcgc gggtaattgc
    26701 tccatccatt gcataaacac ctgcggcggc atcgccttcg caaaaaacca tccctggcaa
    26761 tagcgcacgc cgcgtttgcg cagccagtta acctgctcct ccgtttcgac gccttcagcg
    26821 atcgttttta accccaggct gtgcgccagc tcgatgatat gttccgcaat caaatgactg
    26881 gttttatggg tggtcagcgt ctcaacaaac gatttgtcga ttttcaaaat atcgacattc
    26941 aatgatttaa ggttatgcaa gttagagtag ccaataccaa aatcatcaat tgccacttcg
    27001 taaccggcct ggcggaaagc cagaataatc ggcgtcattt tgtcgacatc aagaaatgca
    27061 tgttcagtca cttcaaattt aatttgctgt ggacgcaccg cgtattgctc tgttttctga
    27121 ttgattcgcg ctatcaaccg tgacgtatga aaatcggagg ccgacagatt aatagaaaca
    27181 tagcgatcag catgtgttgc aaggtacgcg cccagatcgc ggaagacatt atcaataaca
    27241 taatcagtta cctgttctat catcccctcc ttttctgcca gcggaataaa ctctgccgga
    27301 ttcattactt gcccctgctc accaggccaa cgtaacaacg cttcagcgcc gatacatttt
    27361 tctgttttga tatcgattat tggctggtaa taaagacaaa gttgatgttt ttctagggcg
    27421 cgttgcaatt tgcgtttggg agacaaatag ttttgtcgaa tacgtagcca gagtaatagc
    27481 agaacaagac tccccaaaat accggcgggt aacgcaaaaa taagatgatt ataaaaatgg
    27541 gctataagac gttgatatga agtcgcaaca atggctgcaa ttgggcgttt tgtcgaataa
    27601 actgtcgcat ataaatagcc atttctttgt acggttaaat ccttcaaatg aatcagcgga
    27661 gaaaacgttg ctgccgaggc ctcgttgctt aacgagaaaa aggttttcgt caccgtatca
    27721 tacacacccc attgcaatgt cgggtcatca gacatcactt cactccagaa gagagggttg
    27781 ataaccgcca cataatttcc ccgctgcata taggtcattt tatagccaga gaaaaaaggc
    27841 gtatcgcggt aataatagat agaaacgtta gggtcacgct tataatcggc cggtgcaatc
    27901 gtatagccgt ttacaggcgc tatcagcgat gagcataaaa aatggttatc atgggcatag
    27961 atcaattcat taatataaag atagccacga ataatattta acattcgctt ttgatgggct
    28021 ggagagcaaa cttgcccctg atagcgttct gctgcatcgc tcactaaatc tgcctggcga
    28081 atgaccagct cggatttatc cagtgccagt tgagcaaagg ttcggagctg attattcact
    28141 tctgattttg cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa gagaactaaa
    28201 aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat tgctatcaat
    28261 ttcctgcctg tttatacccg gccattgcaa ggtgaaaccc tggctaccga cgtctttaga
    28321 taaatcttac ggtataaata gcacttttgc ctgatttgaa attgatacct ttttaatcgt
    28381 cacctggatg aaaataattc attgatattc aaaattaatt ttctacagga atacagaggc
    28441 ggcgggaaga tataccctta atgccactga aaaaccacat aatctgtaca gcaataaaag
    28501 tcacgaccta atctggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa
    28561 caaacagagt gtttcatttt tgtgttctgc aagccccgcg atagccagag tgtaccacgc
    28621 ctcccgtgaa caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat
    28681 ggctaccctc accactggcg tggttcttct tcgctggcaa cttcttagtg ccgtaatgat
    28741 gtttctggcc agcacgctca acatccgttt tcgtcggtct gattatgtcg ggcttgcagt
    28801 gatcagcagc ggtctgggcg tggtttccgc ctgctggttc gcgatggggt tgcttggcat
    28861 cacaatggcg gatatcaccg ccatctggca caacatcgag tcggtgatga tagaagagat
    28921 gaatcagaca ccgccacaat ggccaatgat tttgacctga tacgtagaag cctccataac
    28981 ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa caatataacc
    29041 tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg gcggctcgtt
    29101 agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt
    29161 gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac caccgatatt
    29221 gccacctgca ggagcgccac caccctgacg accacccagc atctgcatag tgccgccaac
    29281 gttcaccaca acttctgtgg tgtagcgatc ctgaccagat tgatcggtcc atttacgggt
    29341 acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc tggccacttc
    29401 tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgctctttca tctcgccggt
    29461 cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg cgccaccatt
    29521 tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac
    29581 gcctctgctg gccatgttcg tgtctcctga aaaaaaatcg ttctgaataa gtgtaaacgt
    29641 gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa
    29701 acctggccag acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat
    29761 tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg
    29821 caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca
    29881 cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg
    29941 ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc
    30001 gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggaaaagc
    30061 cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt
    30121 ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtttgt
    30181 tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa
    30241 ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc
    30301 tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa
    30361 gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga
    30421 aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg
    30481 acgatcttac ccaacgtctt gccgagtcgt ttgaaaccgc gctggagctt tccggtggta
    30541 ccgcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact
    30601 tcgcctgccc aatttgcggc tacagtatgc gcgagctgga gccgcgactg ttttcgttta
    30661 acaacccggc aggtgcctgc ccgacctgtg acggccttgg tgtacaacaa tatttcgatc
    30721 ctgaccgcgt gatccaaaac cccgagctat cgctggctgg cggtgcgatc cgtggctggg
    30781 atcgccgcaa cttctattat ttccagatgc tgaaatcgct ggcagatcac tataagttcg
    30841 acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt
    30901 ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc tccattcgtc
    30961 gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acggagtcca
    31021 gcgcggtacg cgaagaatta gccaagttta tcagtaatcg tccgtgtgcc agctgcgaag
    31081 ggacgcgtct gcgtcgggaa gcgcgccacg tgtatgtcga gaatacgccg ctgcccgcca
    31141 tctccgacat gagcattggt catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc
    31201 agcgggcgaa gattgcggaa aaaattctta aagagatcgg cgatcgtttg aaattcctcg
    31261 ttaacgtcgg cctgaattac ctgacacttt cccgctcggc agaaacgctt tctggcggtg
    31321 aagcacagcg tattcgtctg gcgagccaga ttggtgcggg cctggttggt gttatgtatg
    31381 tactggacga gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc
    31441 ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcga
    31501 ttcgcgccgc tgaccatgtg atcgacattg gcccgggcgc aggtgtacac ggcggtgaag
    31561 tggtcgcgga aggtccgctg gaagcgatta tggcggtgcc ggagtcgttg accgggcagt
    31621 acatgagcgg taaacgcaag attgaagtac cgaagaaacg cgttccggcg aatccggaaa
    31681 aagtgctgaa gctgacaggc gcacgcggta ataacctgaa agacgtgacg ctgacgctgc
    31741 cagtcggtct gtttacctgc atcacagggg tttcaggttc cggtaaatcg acgctgatta
    31801 acgacacact gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc gccgaaccgg
    31861 caccgtatcg cgatattcag ggactggagc atttcgacaa agtgatcgat atcgaccaaa
    31921 gcccaattgg tcgtactccg cgttctaacc cggcgaccta taccggcgtg tttacgcctg
    31981 tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg cggctatacg ccgggacgtt
    32041 tcagctttaa cgtccgtggc ggacgctgcg aggcctgtca gggcgatggc gtgatcaaag
    32101 tggagatgca cttcctgccg gatatctacg tgccgtgcga ccagtgcaaa ggtaaacgct
    32161 ataaccgtga aacgctggag attaagtaca aaggcaaaac catccacgaa gtgctggata
    32221 tgaccatcga agaggcgcgt gagttctttg atgcggtgcc agctctggcg cgtaagctgc
    32281 aaacgttgat ggacgttggt ctgacgtaca ttcgcctggg gcagtccgca accacacttt
    32341 ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc
    32401 agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac
    32461 tgctcgacgt actgcataaa ctgcgcgatc agggcaacac cattgtggtg attgagcaca
    32521 atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg
    32581 gtggcggcga gatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc gaagcatcac
    32641 acacggcacg cttccttaag ccgatgctgt aatcgttaag gccgctttct gagcggcctt
    32701 ttcctttcag agttgcacca gcaatttacg tttttcttcc ggcagtaaat tcaccgcctg
    32761 ctgataagac gcatccacca gataatagat ttgcgaatct ggcagcgaac cgtcgagata
    32821 cacggtgctc cagtgcgctt tattcagatg gcggcttgga cgcacatcgc tgtgctgctg
    32881 acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac
    32941 ttctttcacc atcgcaaaca gtacatcttc aactttgatc tgcgtcgctt tccagtcgtt
    33001 atgcacgctc tgttctgcgc ctggttttgc catgcaatat tgtagcaact ccgaaatggt
    33061 cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg
    33121 ttcccccagc caaatgcctt gccaggtgcc ggtctgaata cgccctttat gcaccggcaa
    33181 tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc
    33241 atagtcatgc tcatagtttc cattatcggg aacggtgcgg aggaaaaaac gctccatgtc
    33301 gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag
    33361 caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat tcagaatttc
    33421 atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca
    33481 catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata
    33541 aaggtgtgat taaaaaaaca gcattcggga gagtgtcacg ctctcccgtt ctgtcagtat
    33601 tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta
    33661 gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc agcggtaata
    33721 tcgttatcgg aatcgccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt
    33781 gtattttgcc ctggtttatc gcccgcaaag atcaccggat tcatgttggt ggcaggaata
    33841 tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct acgaccagtc
    33901 acaaagaaga tcgcgtcacc acggcgtaca tgcatatcaa tcagctggcg agcgacctct
    33961 tttggaatgc tgaattcatc ccagccgttg ttcatttttt cccagaatac agggtttttc
    34021 agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaagcc cgggctggaa
    34081 aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta
    34141 ttttcaattt gtgcgaccga aacccaatga attggtgcct gttcagcaag cctggcaacg
    34201 ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga
    34261 gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa
    34321 tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac
    34381 gccagctgca ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt
    34441 atgtttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt tattactcga
    34501 tgtaaggata cattaatttt acgtttatta tgatgaatcc tctgcaaatg agcgaaattt
    34561 gatagaaaat ttatctgctg tacgatttaa tgaacttaat gatacagcat tacaaaaaca
    34621 atcgaagttt ataaaataac atttttataa agatgatttc tgattgacca gaaccctacc
    34681 ctacaatggt gactaatttt attactcccg aaggagatga tgacatgaat atatcatatg
    34741 tgaatagcaa caaaacgaca tctttacctg tagagctaga tacactaaat aacaaagata
    34801 tctcttatgc
//
LOCUS       DAZGCQ010000051        32915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000051 DAZGCQ010000000
VERSION     DAZGCQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.051"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..456
                     /locus_tag="ACHK07_002625"
     CDS             130..456
                     /locus_tag="ACHK07_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102939.1"
                     /translation="MQTITTHEQLLVDGKITERTATHIVTGAHGYETLCTSGYNIRYN
                     EKQELVESCEKIAEGELPVTCPTCFAVWQDVHKFTPGDFDTESGKGDFADTNLTEIMI
                     GKMSDT"
     gene            complement(761..1078)
                     /locus_tag="ACHK07_002626"
     CDS             complement(761..1078)
                     /locus_tag="ACHK07_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO8102940.1"
                     /translation="MKQFSVYKNKSRNKQAYPYFIDIQAEMLAHLNTRLVMPLTPKDN
                     SNSQVKTLTPVININQVDYVVLTTMITTTDAKNLNSDNVVMDASYLRDQLVSAIDMMI
                     LGI"
     gene            complement(1078..1323)
                     /locus_tag="ACHK07_002627"
     CDS             complement(1078..1323)
                     /locus_tag="ACHK07_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO8102941.1"
                     /translation="MRTNVAVKKRSINVYLNAGLLEEAKDLDLNVSAISNKALEKAVR
                     ERKRERWIEENRAGIEALNRFVEDTGIFSDDENFGVI"
     gene            complement(1687..2751)
                     /locus_tag="ACHK07_002628"
     CDS             complement(1687..2751)
                     /locus_tag="ACHK07_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO8102942.1"
                     /translation="MDKKTKTYPFGDIEFFDQLEEHAWDQIHDGMLPIMDLYLDPTVT
                     DIFINRYDNIYTSVNGKYVKTDSSFSSERALKAWIDQIATVLRQPFSTSGNYKYGDVI
                     EPVLDARFPDGSRLMCTDPVISPQGSTVSLRKVPKQILEEKDFINSGMLTRDILDYLI
                     SVIQKRKNFIVAGNTGSGKTSLLRLLARYIDTMERVITAEDTQELHIHRYFPLGIALE
                     AAHRKDVNADLPSLVHLTMRGNPDRVWVGEVRTAEAIAAYYMVLASGTIGNATTLHSV
                     TPMGAIRKMTWLMSSYLSIDFQIAQDLIMSEVDVIIQCRRTSKHGRRITDIVEIREGK
                     IVPIFKFDEEQEVHVRFDKK"
     gene            complement(2808..4673)
                     /locus_tag="ACHK07_002629"
     CDS             complement(2808..4673)
                     /locus_tag="ACHK07_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102943.1"
                     /translation="MQANDEFLRLVFENIWPWWTYAWVLVFSLLCVFTFSGHAEKSGD
                     KSTLRFIIEAARKKLLVAWILCTLLFFLFVYIIAWKHFGEDSYKASLYMELLYERIGD
                     KSFSFGDYCGIAFVLLFPYACQIINRRLIRPKLSSWLRRFRVSQTSDTMSDIRVEKGK
                     YSPKNFNPLDYYKDGHFFLGLDENDKAIYVTDDYLSKVNIKILGATQTGKGVIQGVIA
                     DQAILKNWGLWFWDQKPDKFIYSIMKQRCESEGREIYEVDLNGEGIGDYAPFKNGTKR
                     EILDRFFNAFSLETGGTDADYYKNNAQEVMYYIYTFWDRSLNDLQKLLSGSDDRIPEE
                     KQEWIAKNGANIRTQLNKWLELPCLSPADGQGLNVTELVKSGAVIYVKGSTKDKVVRA
                     VLKSMLREWSAAVIREQPKNHVLGIIDEARFVISSEVADSLATILSSNASLSIAYQER
                     DDLLNIPDEKPQIAQSIKKGTETNTNLTLVYRCNDETAEWVAKNSGTTAKSLTKLEEV
                     ETDGYGAETWKGRRMIGQEEDYYIPVNTVLAVEERVGIMQTMFELSRVLFTCWVPVQR
                     FYTLPARPQTEHDNVQDCIKNDGSISPSDEDYFNPDMISDEELAMINKTVSSDSK"
     gene            complement(4700..5392)
                     /locus_tag="ACHK07_002630"
     CDS             complement(4700..5392)
                     /locus_tag="ACHK07_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102944.1"
                     /translation="MIYRFKTDFMRQIAMLFSLGYTRWFGGEIPLNPHYKFVSLKARF
                     DELYAINATAQQRFRRREKGVANCKFLVWFDEKNATLLWVLLATEGDVYNEEKFYDGM
                     NKKTRISVSGYELKTVPHRGRKPSVSWAMKKETYEKWHADIKKSIRTRNDENIEKLWY
                     SLRRVAGFSELRRQVFKLQRYAMTEWKRSRRGEYPYEKIFVGWLGRFKQSETITDEEL
                     LLISKSKKADIF"
     gene            complement(6713..6928)
                     /locus_tag="ACHK07_002631"
     CDS             complement(6713..6928)
                     /locus_tag="ACHK07_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102945.1"
                     /translation="MSDKIQEITQKIEKLQIKLSKIEEEKNKIQSKLVKLEEEKKSIQ
                     RKLILQEKREELEMLLDKESFDILFNK"
     gene            7009..7467
                     /locus_tag="ACHK07_002632"
     CDS             7009..7467
                     /locus_tag="ACHK07_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102946.1"
                     /translation="MFRLAKPEDYEFIPDINLWELSFDKRPVRGVRCEDPVLGAQQYN
                     DTQQSILLNRQRQKLRKQNFFSLENISWDGIFDWCLSKGTQEECYLIVKLYYAKNKDE
                     HYSTLSKLKKCEGFYHESDLYLWVSMFFMSGNKIASTDKLTLVEEKGGYI"
     gene            7464..8060
                     /locus_tag="ACHK07_002633"
     CDS             7464..8060
                     /locus_tag="ACHK07_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102947.1"
                     /translation="MMERKQLNLRINDQFLIGEIDNKLHSEKLIHKHHVTDAINTIKY
                     LEILFTSSNLKRYKNKEHYIKHIVAKSLLPKAEEIIRIQKDEFSSHYEHLKQTVSEIP
                     NYKDIYNHINNENKKIKIPVLISPVVMMMISLITLTDNIIKQQRILYLCGEISKKDFY
                     SNRRVVLKKFSNVRLKLHHNRKEYSEKLSTLIEKKGMQ"
     gene            complement(8628..8978)
                     /locus_tag="ACHK07_002634"
     CDS             complement(8628..8978)
                     /locus_tag="ACHK07_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102948.1"
                     /translation="MKDNQNQSLQVGDATIRIGDIFHTHWGYEQTNIEFYQVVSLHGK
                     KTVGLREIAREVVEHTSWCSAEIRPVPDKFIDEAIHKRRVIAKWGSVLVRFSDVTNAW
                     RTSPEALHHCSWGY"
     gene            complement(9165..9668)
                     /locus_tag="ACHK07_002635"
     CDS             complement(9165..9668)
                     /locus_tag="ACHK07_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102949.1"
                     /translation="MHQEQDIADALHESLAYFNRYCRYSLKRLAADYQEEIDNYQDDV
                     WEAPQRAARLSAAVKNYKTSRMIEFIFYMAIKLNLDLTPLVVKRLCSCLFQRTGSQEL
                     IVEIFGKKQRVNRSADNTMERINDVTERYRKSATRHWRKTLSDIEKVKKGYQKEIRAR
                     RKKKSGK"
     gene            10040..10339
                     /locus_tag="ACHK07_002636"
     CDS             10040..10339
                     /locus_tag="ACHK07_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8102950.1"
                     /translation="MATYRELLAKESPEMQAHVADRVEKASIQLALSALRDELNISQT
                     ELATMMGVKQPSVARMEQTDNDPRISTLKRYVKALGGELSLDVTLPTGKRMAFHL"
     gene            10358..10648
                     /locus_tag="ACHK07_002637"
     CDS             10358..10648
                     /locus_tag="ACHK07_002637"
                     /inference="COORDINATES: protein motif:HMM:NF036629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFO8102951.1"
                     /translation="MRTLEEVIAAQSPDSQAIIKAMTDKMRLEIGAKTMNTVQIPTGT
                     IKTFGLLGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE"
     gene            10652..10930
                     /locus_tag="ACHK07_002638"
     CDS             10652..10930
                     /locus_tag="ACHK07_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFO8102952.1"
                     /translation="MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSL
                     YTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRIEQWSDFTSSIKG"
     gene            complement(11076..11567)
                     /gene="yjeJ"
                     /locus_tag="ACHK07_002639"
     CDS             complement(11076..11567)
                     /gene="yjeJ"
                     /locus_tag="ACHK07_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8102953.1"
                     /translation="MEYTPIALSPGAMLHKGKFIGYVMKIRFSECSGDHLFYLRYDMV
                     VELARALARKTHQMYLKGTFSTDESSQATLEMEKDYKQHDIHLTSDEISSPSVNNFVA
                     LLDYNVRDASLLLSLIKNDNSKIDISIPEAAVEIFTGYMMNTVIKAGGEALSEKLISD
                     IGV"
     gene            complement(11596..11805)
                     /locus_tag="ACHK07_002640"
     CDS             complement(11596..11805)
                     /locus_tag="ACHK07_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="HFO8102954.1"
                     /translation="MSKMKLGNHKATNTALDALKKPAGATRKLQMNIPEELHLAFKLA
                     CVEDGVDMTQVNIELIQNWLKSRNL"
     gene            complement(11802..12425)
                     /locus_tag="ACHK07_002641"
     CDS             complement(11802..12425)
                     /locus_tag="ACHK07_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8102955.1"
                     /translation="MPKVIAFLNGKGGVSKTTSAVNIATALARKGYKVVVVDTDPQGS
                     VSNWYDDSKCVFDVAEATTEKEIYRLRKALPLHDFIIIDGAAAISAISAAAVMVSDLV
                     IIPVTPSPLDFAACGAILAVIEARQDLRPVIARFLITKKVQQTHMLSLLKESIAETSI
                     PALKTGTSYRQSYIKSMLDGGTIFDTKDSAAKGEISVITEEILGLLK"
     gene            complement(12745..13095)
                     /locus_tag="ACHK07_002642"
     CDS             complement(12745..13095)
                     /locus_tag="ACHK07_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102956.1"
                     /translation="MKIKLGSGSVSVNGVVYEGKGKIISILNNEVIIDGVVHGKAVAL
                     PEITVIVNGHVDSLHTELGNIIINGNAGMVKTASGDVSCENVSGNVESMSGNVNCKNV
                     YGGVKTLSGNIYKE"
     gene            complement(13128..13403)
                     /locus_tag="ACHK07_002643"
     CDS             complement(13128..13403)
                     /locus_tag="ACHK07_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102957.1"
                     /translation="MSQYRLTGKSASRSYELLVGWDTPLSRFFLVIEPELDEPVYSNI
                     YEKDPSSLTLEFFQSVLERYGIENVSLLPGHESGLYEKLHDDRRNNN"
     gene            13584..14078
                     /locus_tag="ACHK07_002644"
     CDS             13584..14078
                     /locus_tag="ACHK07_002644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102958.1"
                     /translation="MQEGIAIVKVNNIEVGSMPISQYEEIVKSVKKDWRTHVASVFSY
                     IGFAWRITLKLWHYFVHGFAIFIASIMFYSIYHQAELIHLITFLRNSPSENIAEIIRV
                     ITYYCIALTVIAVAISIAAKGIPIFVSATEIAINKKIREVMEVPTEGQVSITFKKDGV
                     YSVR"
     gene            15705..15926
                     /locus_tag="ACHK07_002645"
     CDS             15705..15926
                     /locus_tag="ACHK07_002645"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8102959.1"
                     /translation="MGATPQRTQAGLQAARARGRKGGRPKTLSKDKQALAVQLYNEKK
                     HTVAQICVLMGISRPTLYKYIESARLFKK"
     gene            complement(16108..16425)
                     /locus_tag="ACHK07_002646"
     CDS             complement(16108..16425)
                     /locus_tag="ACHK07_002646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102960.1"
                     /translation="MIINREKFEIIRKIIHDTLLGNIALVVIALMSPVLLDKIAEPFN
                     IIIASAMSISFIFILCWLASSIWLYAGELAKNKSLRCTLFILWALSAEGSIILTARDI
                     ILW"
     gene            complement(16723..17211)
                     /locus_tag="ACHK07_002647"
     CDS             complement(16723..17211)
                     /locus_tag="ACHK07_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102961.1"
                     /translation="MNNLRDKKSNPVITLIVVVISLFLLPAIVAGLGTMGVIYIDGLR
                     ELPSFCIKAISVAIWMLVVAYLMCRIMKAKKDFIRLPAKLTLIGAVLLPLISSFNNVV
                     TTMSSPDVSTRTDFHAALIKGLDTMESEFMFIVPALLCAIASIMLAVGMINILRNRYM
                     GR"
     gene            complement(17422..18072)
                     /locus_tag="ACHK07_002648"
     CDS             complement(17422..18072)
                     /locus_tag="ACHK07_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102962.1"
                     /translation="MMVNSKKIVIIALSSLLLAGCDSSKDANKANFEKAINTKLEKEC
                     ITISPLGRFDSKPYPVQLSVAQTNRYTSQEQADEINTKEFSMFDTLVKAGLLTVKDTL
                     VDETIGFSKTGKKVPGREYALTENGKKYLKSPERPDFCIGHYKVDEIIDFTEPGDAMG
                     MKITRVNYTFSPTNIAEWAKSDDIQAAFPLLKFNLKEKQKNRLSLVLKNDGWSAER"
     gene            complement(18138..18446)
                     /locus_tag="ACHK07_002649"
     CDS             complement(18138..18446)
                     /locus_tag="ACHK07_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HFO8102963.1"
                     /translation="METFDTDKFRSELDLLSKKIMPGCGLVFELYQRRLSAAIDEFVA
                     RLPKEQHAQAFELARQEFDYLSAEEIADEIRRDAEKGYCCHGLDRDCCPLGCGDLDDY
                     "
     gene            complement(19137..19772)
                     /locus_tag="ACHK07_002650"
     CDS             complement(19137..19772)
                     /locus_tag="ACHK07_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="HFO8102964.1"
                     /translation="MSRKRRRFLNRWIKSLATVAGRVKIKNELRRRIRHNRYWVNEAN
                     KFGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGRKSISRASRGCDR
                     LVWLNAIITDKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLLKADPNEM
                     IYSGDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHPPTPIPV"
     gene            complement(20352..20615)
                     /locus_tag="ACHK07_002651"
     CDS             complement(20352..20615)
                     /locus_tag="ACHK07_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO8102965.1"
                     /translation="MMVSILVVSNNGREELIDFNPDHDLVHIIKSYRTPENRMVCIIQ
                     DGKRILRWDRSYASRAKNHWRKADPDSFEILGSVEYLRYVGQG"
     gene            complement(20856..21824)
                     /locus_tag="ACHK07_002652"
     CDS             complement(20856..21824)
                     /locus_tag="ACHK07_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO8102966.1"
                     /translation="MISPAIDYFAANLPYRPYHANELLYGLRIDKAAKAVLARYIQHN
                     PPHAMYWLVFDVDRPVAAIDWNDLNAPTPNFTVKNPVNGHAHLFYGLKTAVRVAKDGS
                     IKAIEFAAAVERGLRDKLKSDTGYAGLICKNPLHTYWQVVEWRGELYELAELADYVDL
                     YAPTAANDEQAFDIDFSYGLGRNCWLFNLTRIWSYTAIRQGWPDFPQWKEAVIQRVEM
                     YNVQLSVPLSHKECVCIGSSIAKWTHKRFTEAGFEQYVADTHTSEIQARRGRKNRSEV
                     QAAKGRKGGKISKRGIVEDSARSLKPWVELGISRATYYRRRANETK"
     gene            complement(22052..22495)
                     /gene="ssb"
                     /locus_tag="ACHK07_002653"
     CDS             complement(22052..22495)
                     /gene="ssb"
                     /locus_tag="ACHK07_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8102967.1"
                     /translation="MARKGVNEVTLVGFVGNAPVISQASSGDKIAVISVATTETWTDR
                     KTGAEKEKTEWHRVVIFGKTAEITEQYVKKGTLLYIRGKLQTRKWLDRDNIERYSTEI
                     IVNPAGGEMQMLGSRQDKVSGGKAGNDIPENGAGSTDKQQSNETD"
     gene            complement(22511..22870)
                     /locus_tag="ACHK07_002654"
     CDS             complement(22511..22870)
                     /locus_tag="ACHK07_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO8102968.1"
                     /translation="MTKIKILILAAFVSFGTVHSASAENGSDALSLTGEFLEQPVNSS
                     DPCTVFLCMAGMLKGESPGECSGAKKKFFSIIKRKHGSFHPGRTFNARKAFLGDCPSA
                     PGDIVGKILDKYGKLRW"
     gene            complement(22873..23529)
                     /locus_tag="ACHK07_002655"
     CDS             complement(22873..23529)
                     /locus_tag="ACHK07_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO8102969.1"
                     /translation="MLAFLISGGISASTDEEILLASITAPGVTSFTDESPSSEWITAM
                     QGRWGSTTDMKDMRSLREIREAVIAEINAVSEPVVEKTGPENKESTTGIKISEKIQSI
                     INKYAEKYQISETLLKALIKTESGFNPNAVSRKGAKGLMQLMPVHTKRHNADPFDVET
                     NVKIGTGHLSYLLDKYGDLRLALAAYNAGEGAVDKYGGIPPYKETQQYVNKIMAMLGG
                     "
     gene            complement(23547..23756)
                     /locus_tag="ACHK07_002656"
     CDS             complement(23547..23756)
                     /locus_tag="ACHK07_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102970.1"
                     /translation="MRIIFVAFLAIATVILCLFMTGCAQRYESGHDQLFELSERHIIY
                     RDLTVPASMSNLPEKQQISVPGIEP"
     gene            complement(23768..24169)
                     /locus_tag="ACHK07_002657"
     CDS             complement(23768..24169)
                     /locus_tag="ACHK07_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102971.1"
                     /translation="MRESAPVQIAQQDVYVQGNFQELQPLDRNQKDPRWQYIDPLSVY
                     MRTVWPDNIRTIRAAVNYLLEPTGYRLVTSHPAPREASLLVDKPLPPIARMQRTMPVV
                     DALQLLVGLDNYVVIDRAHRLVSFQKNGGGV"
     gene            complement(24240..25646)
                     /locus_tag="ACHK07_002658"
     CDS             complement(24240..25646)
                     /locus_tag="ACHK07_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO8102972.1"
                     /translation="MEERQEKVEEKPATYRKKIQYYAFGTFGVILIILFINMVISIAS
                     RNEEEPKENAKQETASTAPNTTEKFNALLKNSGARPSASVNQANTVGNKDSASNGNPN
                     SETDKKPVHPYSREKEASQERGARASDAGSPDEIRARFKADEINRALRARSDKTNFDS
                     TKNTVAGSGNAYAPAASFTGNRTNAQRISDINAEQNGLQERIQMMESKVRADEAALPG
                     RLEEARIRGEQQFAEARQGGAGSNATSVTKGSTQSVPSGVVGYTASNPYGASVDGMMK
                     LPVGTVLNAITTFTAISDYSGGSMKAMLTHDIYDATHSYILAPKGSEFVIRVVKASQV
                     NEILQSRIGFQVTWLVLPDGNRVDFKTSSSLDRMGTPAVEGDEVDRHLLAQFLGVAAY
                     ALVGTKTSYEGSGDSNESFAGNFGEGARTQAGNIAQKYLQVVPTTTLHAGAPIRVFTE
                     DEIYMKPWRNIYETDYTG"
     gene            complement(25649..26482)
                     /locus_tag="ACHK07_002659"
     CDS             complement(25649..26482)
                     /locus_tag="ACHK07_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO8102973.1"
                     /translation="MLAKRTLLFSGVLAVVMTTFSATAQQCRVVNWRPGTIVKVQSAL
                     YLGTRIELPEGSMLLTKEPFSSNALWASTGAANQVMIQPTSEEPEGLKTMVTVFMTNG
                     QTLDIEAVRVSQNQNQQCVQIRGAGMRSADRSALNTAYSMGASGADVARLQNEVNTLR
                     SNRESEVKNAVLEALRRYRYFIYTRYNWTTGKGFSGKNMISDVYDDGRFTYIRIAKPN
                     RGLLAVHADVGGKPAVIPTKYDDAYAIYHMSGIYPKFTLTLDGVKLEVSRADNATDGE
                     S"
     gene            complement(26495..27214)
                     /locus_tag="ACHK07_002660"
     CDS             complement(26495..27214)
                     /locus_tag="ACHK07_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFO8102974.1"
                     /translation="MLLEKKEVEKVNRTAFKDRVGELKKDRNLFFWIALLLAGTTIYQ
                     SYSVQEAIEKSQNTTEVVWVKLLPDGSHKISQFTPDNEQPIYTPTVNSLLSKYVQKRY
                     GQLKETIERDYAEAAVFMSPVLTGEFADPKGFNVAQKVVDIQADMAAKRIDIEIKDID
                     HYDAIEGQFDGDVKPVIRTLVTYQEITRDYQGKEISSVQQMRRLQWTLLQRNELSKQS
                     LDWLNINPLGIQIIDDKSVQQ"
     gene            complement(27224..28195)
                     /locus_tag="ACHK07_002661"
     CDS             complement(27224..28195)
                     /locus_tag="ACHK07_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102975.1"
                     /translation="MSLINRLLFVVLFLSVSFYASAETAMRTNFQNGVSEMVNKILNS
                     PDSDLRVWTIAASTFLAAVTAFVYFWRFVENGGALVDLFTLAIMFMLSMSLLASYSMV
                     VDTITGAFYAISNTLQKLALGNIKPSFLQNFVEGVLTKAISAPSVDIFDGMNMVGMAL
                     LWEICSAILSVAIYLADVWIDVGTAIAKITGVMFIPFLVAPATRGIFDGWFKFYIGWG
                     LAAVVLKITSIVVMIMIRASINSAGSFKGAGDVIASDGTVNGVLNITANNISLLRDIC
                     AYSVIAAIMVFSCFIFAYMIGSGVGSASQGLNNMAVKLAKKAAKALL"
     gene            complement(28255..28725)
                     /locus_tag="ACHK07_002662"
     CDS             complement(28255..28725)
                     /locus_tag="ACHK07_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102976.1"
                     /translation="MMKKSLIVFFIMFSASAMAKTGYVDEHQKEVDLKIDALISKYEK
                     KCEGKRNSNMCKSQAWDKAHFEYEDEFRGRDKYNHEHYDGLTKEQSAAKLHELIKLYK
                     VVEKDERNPEIWAGKLNTLTINREINYIVRKHWPAWINPCDKICAELLLRQIGK"
     gene            complement(28722..29543)
                     /locus_tag="ACHK07_002663"
     CDS             complement(28722..29543)
                     /locus_tag="ACHK07_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8102977.1"
                     /translation="MRKQLAVILFSVMTGLITTPAISSGIPVADAAQILGQARAMMQD
                     IKNYKEYMEQTILNNKELLEAYKQYNQMLEDYKQILREAEGLKDKLSSIDYKNFMDEI
                     ALISNQFDPVYGGGRMVKNTGNEPWDNAYERNLLLNGYGLTDEEYAEMLAAIPYTSDD
                     RERARQMFEYRQRRVVEGISKDAFVGTLQIQLDEQKDTLQELQKRRLRLGDNDTTASI
                     QFLAEQNEQLLKQIQSLQLQQAEAMKYSGRLDEHYFNKRAQQEELKARQLQRAWQ"
     gene            complement(29540..32140)
                     /locus_tag="ACHK07_002664"
     CDS             complement(29540..32140)
                     /locus_tag="ACHK07_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO8102978.1"
                     /translation="MGQSYNEHLNLAGIEQHSGQVYTYTGGAFGAIELTGVDPTGVTP
                     SQGSRISLLLRNIVQLLPLSVTLTQYYIHNDGVKIRLTGRDNQRSQLLSMRRQAYLNK
                     CRNLNASRIIWTLDVHPEENINRLASAEFLKNLFNSMFDDTARRKVRLALSNRESFII
                     ERQAFEKQCLLLKETLRDLDLRLSFISEDNRILDSEGIWRLQKFLATFNHHWLAPRRK
                     VTVPNDMWDCLALEGDDIEAITWRGVDMLKIQGVRPVYVRLASVLKIGDESVPECAWA
                     LTNGRPVLQRGNYVYFMRFTPASAFKKSLMISGKENELVRTQISLSDLMTDSTSKDIL
                     DEKLRRNSHLMKMREELENISYSDERLGICQAGIAVFNTDPDKLLETCADINRSVSQT
                     MQVVWETVAQLDAYKVMQPAYSRNTFRALQLNASQMGAASLFFKSHPGIPEWELNGRM
                     EEAFYILESDDGVPFFFTPKVKEKLLLLGNGATRGGKTFMKNCIASHFIKFGGLYNCL
                     DIDPGSIPMANFFGDDGAAFTLDSDFQCGFNMFSMAQSKDDRVFLAHLIEQLKAMAAE
                     NDSEQDKYLTPDELENITVCASDLLVQKFSPNEGRLSSITLSTLVSKCGQSVKRKLAP
                     FISNGIYGRLFDNKEDAIGVLDKPVAVYNLSGVKDNHNLARLVHREIFFRVVRLFESP
                     EYRTIPKFFEIDEAQYTLSVPHAAEFATQKARTWFKHGGGMGFWTQNPEHYSSLDEWT
                     TLRSSASVFIFMPDNEATVKAFHDAYGFDEEEVNIIRSLKVKQQSYIKIPDMQIAKVV
                     NLFVEAEQYAVCTSTSNEAALAAEIWKRESDPDVAVRQIVEGLITKCNKDLIPTPTET
                     EKDKTEGFFL"
     gene            complement(32152..32499)
                     /locus_tag="ACHK07_002665"
     CDS             complement(32152..32499)
                     /locus_tag="ACHK07_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102979.1"
                     /translation="MLNRISRKISTFQIMFYVALVTGSVTPAMAAGNSTVKLPTPDDS
                     FLQKIVNWMQSILDLVGGAGALFFAFVSGFACIVLWGLAPQQSQAIGKLFRAAVAAIA
                     AFNLAFFIAWLQK"
     gene            complement(32529..>32915)
                     /locus_tag="ACHK07_002666"
     CDS             complement(32529..>32915)
                     /locus_tag="ACHK07_002666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102980.1"
                     /translation="NINFTKRKNILRHSVRNQMAEIKGKITNPALVNAKVFYNRRTKL
                     GIPTEVFLILMFLNVFAVVLCVMYFSTFSVISLILFVLVSVVPMKFVFHGDPDAHKAW
                     IYGYLSPSRLNNTHAVKRRITFIDAI"
BASE COUNT         9785 a         7591 c         6034 g         9505 t
ORIGIN      
        1 tttcattttt gtgatgtctg tcatgtgttt tttttgagca atgtgaaata agatgctctc
       61 ccgtcatctg acgtatattt ttacggtcgc cccaacaatc acataaacca acaattatga
      121 gagacagaca tgcaaacgat cacaacgcat gaacagttat tagttgatgg aaaaatcaca
      181 gaaagaaccg ccacacatat agtgacgggc gctcacgggt atgaaacgct ttgcacaagc
      241 ggctataaca tcaggtacaa cgagaaacaa gaactggtag aaagttgtga aaagatagcg
      301 gagggcgaac tacctgtaac atgccctacc tgcttcgcag tctggcaaga cgtacataag
      361 ttcacgccag gcgactttga cactgaatcg gggaaaggag attttgctga tacaaatctc
      421 acagaaatca tgattggaaa aatgtcagac acataaaaaa aacaatcatc acataaaaaa
      481 taatgatacg actacgaaac aattcaaaca acattgcaga actgctccaa agtttggaat
      541 atgccgcttt agcggcatgg aaggcgcgaa aaatggtttc atagagtcac agatgccagg
      601 tcgcgccgat acccctaacc agggagtttc atgtcgccag gaaccgcgtc agccccctgc
      661 taagcctgac cgcacggaac ctacagctat tcaggtggta ggtggtcgta gactgtcgat
      721 gttacagcga tattttttac tatttactac cctgcaaaaa ttaaataccc agaatcatca
      781 tgtcaattgc agaaaccagt tgatctcgta aatatgaagc atccataacg acattatcag
      841 agttcagatt ttttgcatcc gttgtcgtta tcatagtagt cagaaccaca taatctacct
      901 ggttgatgtt tattactggc gttaacgtct ttacctgcga gtttgaatta tcttttggcg
      961 ttaaaggcat caccaaccgg gtgttaaggt gtgcaagcat ctctgcctgt atatcaataa
     1021 aataggggta cgcctgttta tttcgcgatt tgttcttgta gacagaaaat tgtttcacta
     1081 aattacccca aaattttcat catctgaaaa aatcccggta tcttctacaa aacggtttaa
     1141 tgcctcaatg ccagcacggt tttcttctat ccagcgttca cgcttacgct cccttaccgc
     1201 tttttcgaga gctttgttag agatagcaga aacattcaga tcaagatctt ttgcttcttc
     1261 aagtaaccct gcatttaagt atacgtttat actacgtttt tttactgcca catttgttct
     1321 cataacaagc cccccacata aattatgcat ataaacatag catataccca aaaccccgga
     1381 tctagaagtg tggaatatgc cgctttagcg gcatggaagg cgcgaaaaat ggcttcatag
     1441 agtcacatat gccaggtcgc gccgataccc ctaaccaggg agtttcatgt cgccaggaac
     1501 cgcgtcagcc ccctgccaag cctgaccgca cggaacctac agctattcag gtggtaggtg
     1561 gtcgtagacc gtcgatgtta cagcaatatt tcacacaaac acaatgatat aagcatataa
     1621 gcatataagc atataagcat ataagcatat aagcatataa gcatataagc atataatgct
     1681 aaatgttcat tttttgtcaa acctgacatg tacttcttgc tcttcatcaa acttgaatat
     1741 aggaacaatt ttcccttcac gtatttcgac gatatctgta attcttcttc catgctttga
     1801 tgttctccgg cactgaataa tcacgtcaac ctctgacata attaaatcct gagctatctg
     1861 aaaatcgata ctgagatagc ttgacataag ccaggtcatt ttgcgtattg cccccatagg
     1921 ggtaacggag tgcagagttg tagcattacc tatagttcca cttgccagaa ccatgtaata
     1981 agcagctata gcctctgccg ttcttacctc cccaacccat acacgatcgg gattaccacg
     2041 catagtcagg tgaaccagag aaggaagatc cgcattcaca tctttacgat gtgcagcttc
     2101 cagagcaatc cctaacggaa aatacctgtg tatatgaagt tcttgagtgt cttcggcggt
     2161 gataacacgc tccatcgtat caatatacct ggcgagcaag cgtaaaagtg atgttttgcc
     2221 agaaccagta ttaccagcaa caataaaatt tttccgtttt tgtataacac taatgagata
     2281 atcaagaata tctctcgtca acataccgga atttataaaa tctttttctt ccagtatttg
     2341 cttaggtact ttacgaagac ttaccgtaga accctgcggt gaaatcaccg ggtctgtaca
     2401 catcaaccgg gaaccatcgg gaaatcgggc atcaagtaca ggctcaatga catcaccata
     2461 tttatagttt cctgacgttg aaaatggctg cctcaacacc gttgcgattt gatcaatcca
     2521 ggctttaagt gcccgttcac tggaaaagga actgtcggtc tttacatact tgccattgac
     2581 agatgtatat atattgtcat acctgtttat aaatatatcg gttactgtcg gatcaagata
     2641 caaatccata attggtagca taccatcatg aatctggtcc catgcatgtt cctctaactg
     2701 gtcaaaaaac tcaatgtcac caaaaggata tgtttttgtc tttttatcca tatgtacacc
     2761 atattattaa ctaaaaaatc tggcatcagc tagatgccag atttatttta tttactgtct
     2821 gacgatactg ttttatttat catcgctagc tcttcatcac taatcatgtc aggattaaag
     2881 taatcctcat cactcggaga aattgaacca tcattcttta tacaatcctg aacattatcg
     2941 tgctccgttt gagggcgggc cggaagtgta taaaaacgtt gaacaggaac ccagcatgta
     3001 aataaaaccc gactgagttc aaacatagtc tgcataatac caactcgttc ttcaactgca
     3061 agaaccgtat ttactggtat atagtaatcc tcttcctgtc caatcattcg tcgtcccttc
     3121 catgtttccg cgccataacc gtcagtttcg acttcttcaa gttttgtcag actcttcgca
     3181 gttgtaccag aatttttggc aacccattca gccgtctcgt cattacaacg ataaacaagt
     3241 gtcaaattgg tatttgtttc cgtccctttt tttattgact gagcaatctg cggtttttca
     3301 tccggtatat ttaaaaggtc atctctctct tgatatgcaa tagacaaact tgcatttgag
     3361 gataatatag ttgccagact atcagcaacc tctgacgaga taacaaaacg ggcctcatca
     3421 attatcccca gcacatgatt ttttggttgt tcacgaataa cagccgcaga ccactcacgc
     3481 aacattgatt ttaagacagc cctgacaacc ttatcttttg tgctaccttt tacataaata
     3541 accgcacctg acttaaccag ttcagttaca tttaatccct gaccatcagc gggagataga
     3601 caaggaagct ctaaccattt attaagctgc gttcgaatat tagctccatt tttagctatc
     3661 cattcctgtt tttcctccgg tattctatca tcactaccag acaacaattt ttgtaaatcg
     3721 tttaacgaac ggtcccaaaa agtatatata tagtacataa cctcctgtgc attatttttg
     3781 taatagtctg catcagtccc gccagtctca agagaaaacg cattaaaaaa cctatccaaa
     3841 atttcacgtt ttgtaccatt cttaaatggc gcataatcac ctataccttc accattcaaa
     3901 tcaacttcat atatttctcg cccctctgac tcacaacgtt gtttcataat cgagtaaata
     3961 aatttatctg gtttctgatc ccagaaccac aacccccaat ttttcaatat tgcctggtct
     4021 gcaataaccc cctgaataac acctttaccc gtttgcgttg cgcctaaaat tttaatattt
     4081 actttagaca aatagtcatc tgtaacgtag attgctttat cattttcatc taatcctaaa
     4141 aagaaatgac catccttata ataatctaaa ggattgaagt ttttaggcga atatttacct
     4201 ttctcaacac gaatatcact catagtatcc gaggtctgag acacacgaaa acgacgaagc
     4261 catgatgata gttttggtct tataagacgg cgattaatta tctgacaagc ataggggaaa
     4321 agtagtacaa atgctatgcc acaataatca ccaaatgaga aagatttgtc accaatccgc
     4381 tcatacaata actccatata aagtgatgct ttatatgaat cttccccaaa gtgtttccac
     4441 gctattatgt acacaaatag aaagaacaaa agagtacata aaatccatgc aacaagtaat
     4501 ttcttcctgg ctgcttcaat aatgaaacgc agagtactct tatcaccact tttttctgca
     4561 tgacccgaaa atgtaaacac acataacaaa gaaaaaacta aaacccaggc atatgtccac
     4621 cagggccata tgttttcaaa aaccaagcga agaaactcat catttgcttg cataagcacc
     4681 tcacgctatg ttgataaaac taaaagatat cagctttttt tgacttcgat attaacaaaa
     4741 gctcttcatc tgtgatggtt tcactttgtt taaatcgtcc taaccatcca acaaaaatct
     4801 tctcataagg atattcacct cttctggaac gtttccactc tgtcattgca tatctctgta
     4861 acttaaatac ctgtctacgt aactcactaa accctgccac cctcctgagt gaataccata
     4921 atttttctat attctcatca tttcttgttc ttatcgactt ctttatatcc gcatgccact
     4981 tttcataagt ttctttcttc atagcccatg aaacgctagg ttttctacct cgatgtggaa
     5041 ctgtttttag ttcataacct gaaacagaaa tacgagtttt tttattcata ccatcataaa
     5101 atttttcttc attgtataca tccccttctg ttgcaagtaa cacccataaa agagttgcat
     5161 ttttttcatc aaaccagaca agaaatttgc agtttgctac tcctttttct cttctcctga
     5221 aacgttgttg tgctgttgca ttgatagcat agagttcatc aaaacgtgct tttagtgaca
     5281 cgaatttata gtgagggttt agaggaattt cgccaccaaa ccaccttgta tatcccagcg
     5341 aaaacagcat ggctatctga cgcataaaat cggttttaaa acggtaaatc atcgaaatcc
     5401 tcataaaagc tatgtagtaa ccgctacata agagaagtca acatatggga ataccttata
     5461 aagagattcc ttgcgctgcg cttacaggtt aaatattata ctgatgtccg gctcgatgcg
     5521 agcctttcgg gcgccggact atcaagcatc gcttacgtaa caaaacgtgc gttgcgagct
     5581 gccaactctc gacgcatttt tgggaagtcg aaagacgaaa aaagccaaaa gccttcgtct
     5641 gccaaacgaa ggtgagttat caggactgcc aaccctgata acagttactg ccgaagccgt
     5701 atgcctgtaa ttgggctggt acggctacga agggcgttcg ggggtgaaac tcccctgtcc
     5761 gacccaatcc ccgccaaagc gtgagactgg gcgacacgga aacgggaacg taggtgacta
     5821 cgtgatccct gttgatcttg aacgggtgaa aatcccgaat atggggtgct tgagaaggta
     5881 caacatattc ccgacaggtg ggctgctgag ggcatcgtga ggtgtcaggg cgttgagggg
     5941 aaacctggaa acgtcggtac tgtgaggggc aacctttgca gatcgctgta aagctcaagc
     6001 ctgtaattgc gtccgaagag gtcgcattcg ggtaagtgat aaccagccat gacttaagtg
     6061 tctggctatg gtgttgacgg caaattatga gtaagggagg tgctttggaa tctcggcccc
     6121 ctgaaagcca gaaatggtaa ggcgtagcct agtagtttgt ctgagccagt ggcgggttat
     6181 cccgacaaga tcagctaacg tgggaagcac tctggaaaat ttcgggtggc accgaaatta
     6241 cattcctgag tgtgggagag tggacgagta ggcgcaaaaa tctacagacg atatagccga
     6301 agtctgtaga tgaatctgaa aatactggag gcaaccagta cagataagta gctgaaaatt
     6361 cacggtagta acatggcaga ggaaacattc atgtttcctc tttttcgtcc tctttctgtt
     6421 ggttcagtaa gaggacttcc caaaaaaacg taggtatatt ttgttatgaa attgactaaa
     6481 aaatcatgtt taacacatgt aagacaacaa cgattttcag tattgatgca ggatcatttc
     6541 tgcaaaaaat tctgcttacg ttgttgataa aacactcaca tgtcctgtct aacgataggg
     6601 cattgtaatg aacaataatc gcgtttttcg cgatcataac taacggtaat aggcactacc
     6661 aatgacaaat tgccctctga taatcagagg gcaattctcc caaaaatcat gctcatttat
     6721 tgaataaaat atcaaaactt tctttgtcta aaagcatttc aagctcttct cttttctcct
     6781 gtagaatcag cttgcgctgt attgattttt tctcttcttc taacttaacc agctttgatt
     6841 gtatcttgtt tttttcctct tcaattttgc tcaattttat ctgcaatttc tcaatttttt
     6901 gtgtgatttc ttggatctta tcgctcatat taaacacctt tgttgaaaaa atttttgttt
     6961 tacggtaaac cagaactacc gaacacacaa cagacaaagg atagcgttat gttccgtctg
     7021 gcgaaaccgg aagattacga gttcatacca gatattaacc tatgggaact gtcatttgac
     7081 aaacgccctg ttcgtggcgt tcgctgtgaa gatcctgttc ttggtgccca gcaatacaac
     7141 gatactcaac aaagcattct tttaaacaga caacgccaga aattaagaaa acagaacttt
     7201 tttagcttag aaaatatctc atgggatgga atattcgatt ggtgtttatc aaagggaaca
     7261 caggaagagt gttaccttat tgtcaaactt tattatgcaa aaaataagga tgaacattac
     7321 agcactttaa gtaaactaaa aaaatgcgaa ggattttacc atgaatcaga tttgtactta
     7381 tgggtcagta tgttttttat gagtggtaac aaaatcgcat caactgataa actgacacta
     7441 gttgaagaaa aaggtgggta tatatgatgg agagaaaaca acttaattta agaataaatg
     7501 atcaatttct tattggagaa attgacaaca aattacatag tgaaaaactt attcacaagc
     7561 atcatgtcac cgatgcgatt aatacaatta aatatcttga aattcttttt acatcaagca
     7621 acctaaaacg ttataaaaac aaagagcact atataaaaca tatagttgca aaatcattat
     7681 tgccaaaagc agaagaaatt atccgtattc aaaaagatga gttttcgtcg cattacgaac
     7741 acttaaaaca aactgtttca gaaataccaa attataaaga tatatacaac catataaata
     7801 atgaaaacaa aaaaataaaa atacctgttc ttatatcacc tgttgttatg atgatgatat
     7861 cccttatcac ccttacagat aacatcatta agcaacaacg gattctttat ctttgtggag
     7921 aaatatcaaa aaaggacttc tatagcaata gaagagttgt tcttaaaaag ttttctaacg
     7981 ttaggctaaa attacatcat aatcgaaaag aatacagcga aaaactttca acactgatag
     8041 aaaaaaaggg aatgcaatag cattccctta cagttcagaa tcagatcaat aacggtctag
     8101 attctgacga atctgacaaa tgaaatattc ggcttcaaac tccgtaatct catttgtcac
     8161 gagcgagtat acaatcaaat gaacaatacc tgctcgtaga tcaattacag catgaataac
     8221 tgccatactc ctgaaaagat atgttctcaa taatcgagaa tacctatcat tttcgcgaag
     8281 attgcattca ttcagcatac catcagcttg agcctcaaca atgaaatcag tgaacgtatc
     8341 aacagaaaca atcgactgat agcatcgttc tcccattaca gaatcaaatg gagatctgca
     8401 agggatcagg tttaacgatg ataaattgtt tataacacta gtcataacca gactcctgta
     8461 ttcagaagct gcgttacgct gccgaagcaa cgtaacgttg cttcgacagt tgtgtgaatg
     8521 ttctttgatg ctgaaacgtc agaacatcaa aattgatgct gcatcatctg acagaaccaa
     8581 tctatcacgc ataaagaaga aggcgcaact gcgccttctc acaacgttta gtatccccag
     8641 gaacaatggt gtaaagcttc tggtgaagtt cgccatgcat tggttacatc actgaaccgt
     8701 acaaggacag acccccattt tgcgataacc cggcgtttat ggatggcttc atcaataaac
     8761 ttatcaggaa cagggcgaat ctctgcacta caccaactgg tgtgctctac gacttcccta
     8821 gcaatctccc gaagccctac tgttttcttt ccatgtagtg aaacaacttg ataaaactcg
     8881 atattagttt gctcatagcc ccaatgggta tgaaaaatgt caccaatacg aatagttgcg
     8941 tcacctacct ggagtgattg gttttgattg tctttcataa cgtttacctc aaacatgagg
     9001 atcacgttac gctgccagag gcaacgtaac gttgcctcga cagagaattg gttaatgata
     9061 ggttacagtc tgcccttccc tatcccgcag acagaaaaaa gatacactaa tgattaaaag
     9121 atatccactc tccccccaca acctttgtag tgagtgagtt cacttcattt accgcttttc
     9181 ttttttctcc gtgcacgtat ttctttctga tagccttttt taactttttc tatatctgat
     9241 aatgtcttac gccaatgtct tgtggccgat ttacgataac gttcagtcac gtcattgatt
     9301 cgttccattg tattatcagc actacgattt acacgctgct tttttccaaa aatctcaaca
     9361 ataagctcct ggctaccagt acgctgaaaa agacaagaac ataaacgctt aacaaccagc
     9421 ggggttaaat caagattgag ctttatagcc atataaaaaa taaactcaat catccgtgag
     9481 gttttatagt tttttaccgc tgcacttaac cgcgctgcgc gctgcggagc ctcccataca
     9541 tcatcctggt agttatcaat ctcttcctga taatccgcag caagcctttt tagcgaatag
     9601 cggcaatatc gattgaaata ggctagggat tcatgaagtg catctgctat atcctgctct
     9661 tggtgcatac tacgccctat gctaacgcta ctatatgtac acattatgca ttagaacttg
     9721 tacaaaacaa tcaatcccca gtctcggcta cctcccttca ccggataact tgcaaatata
     9781 aggctttcac atccaaattc agccaaaaca agaaacgaga ttttattaac aaataagcaa
     9841 cataaaaaac gacgaaaaac gcccaggaaa acatgatctt ttgagcggat ttttcagatc
     9901 tgacaacgaa ctgtgatccg gtggtgctga taacgtccat tatgttaaat agccctgttc
     9961 ttctggaaat tatagtgtat tcgttataat gcagtatata atgaataccg tgaacatctt
    10021 gcaaaactgg agaaataaca tggctaccta cagagaacta ctggctaaag agagtcctga
    10081 aatgcaggcg cacgtagccg accgagttga aaaagccagc attcagctcg cacttagtgc
    10141 tttacgtgat gaactgaaca tatcccaaac cgaactggct acaatgatgg gagtaaaaca
    10201 accatcagtt gccaggatgg agcaaactga caacgatccg agaatatcga cgcttaaacg
    10261 ttatgtaaaa gctttgggcg gcgagttaag cctggacgtg acattgccta caggcaagcg
    10321 tatggctttc cacctgtaaa aaattgggca tcaacagatg agaacattag aagaagtaat
    10381 tgcggcccag tcacctgaca gtcaggctat tatcaaagct atgactgata agatgaggct
    10441 ggaaatcgga gcgaaaacaa tgaatacagt gcaaattcca acaggtacga ttaagacatt
    10501 cgggctgctt ggtattcctt acgaggtagg agagccagcc caagaactac ctgatggtga
    10561 tattcttgtc aacattatac tggttcagac aggtgagaaa gagttgtaca aactttcaaa
    10621 actattacaa gatcctgatg cagagtaagt tatgtacgca atatcatttg atttaaccgt
    10681 tgcagataca gaaaaacacc atccgaaagg cgtgtcacag gcttacaacg atataagttc
    10741 gacccttaaa aggtacggat ttgagcgaat ccagggaagc ctgtacacaa atagcgatga
    10801 aaatatggca aacctttttc aggctatgaa cgcattaaaa gctcttgaat ggttcccaaa
    10861 ttcagtcagg gatatcagag cattcaggat tgaacagtgg tctgacttca cttcgtctat
    10921 aaagggttga aatcctccat ggcctgatgt tgtggaggac ggaaggttaa gcctggatgt
    10981 tgccccctac cggaaagcga gtggccttta agatttggtt caaaaggggc gaaaagcccc
    11041 ttttctgagc gttcgcgttt acctggcagc aataattaaa ctccaatatc tgatataagt
    11101 ttttcagata aagcttcccc ccctgcctta ataacagtat tcatcatata acctgtaaat
    11161 atttccaccg ctgcttctgg tatactaata tcaattttag aattatcatt tttgattagt
    11221 gataataata atgatgcatc ccttacatta taatctaaca atgcaacaaa gttatttaca
    11281 gatggtgacg atatttcatc acttgttaaa tgaatatcat gctgcttata atccttttcc
    11341 atttctaacg tagcctgact actttcatct gtactaaaag tccctttaag atacatctgg
    11401 tgtgtttttc ttgctaatgc tcttgcaagt tcaacaacca tatcatatct caaataaaaa
    11461 agatgatctc cagaacactc ggaaaatctg atcttcatga cataaccaat gaatttccct
    11521 ttatgcaaca tagctcctgg acttaacgct attggtgtat actccataaa tcctccatat
    11581 gcatcataac aaaaactata agttacgact cttcaaccag ttttgaatta gttctatatt
    11641 tacctgtgtc atatcgacac catcttccac acaagctaac ttaaatgcaa ggtgtaactc
    11701 ttctggaata ttcatttgta atttcctggt tgctccagca ggtttcttta gtgcatccag
    11761 agcggtgttt gttgccttat gattcccaag tttcatttta ctcattttaa tagtccaagt
    11821 atttcttctg taatgactga tatttcacct ttagcagcac tatcttttgt atcaaatata
    11881 gtaccaccat ctagcataga ctttatatat gactgcctgt acgacgttcc tgtttttaat
    11941 gctggtatgc ttgtttctgc aatagattct ttcagcaaag atagcatatg cgtttgctgt
    12001 acttttttag taatcaaaaa gcgagcaatc accgggcgta aatcctgccg agcttctatc
    12061 actgccaata tcgcgccaca tgcagcaaaa tcaagcggcg aaggagtaac tgggataatg
    12121 acaagatcag aaaccataac agcagctgcc gatatcgccg aaatcgccgc agcaccatca
    12181 ataatgataa aatcgtgcaa aggaagcgcc ttccgcaaac ggtaaatttc tttttctgtc
    12241 gtagcttctg ctacatcaaa aacacactta ctgtcatcat accagttact aacacttccc
    12301 tgaggatcgg tatccacaac gacaactttg tatcccttac gagcaagagc agtagcaata
    12361 ttgactgccg aggttgtctt agacaccccc cccttaccat ttaaaaatgc tataacttta
    12421 ggcatttgcc cctccccttt gttcacatag tacctctgcc aaacattcta ataatctcat
    12481 actcataaaa ttatgcaatc ataaaatcac ttgattttat gattatcgaa tcaagtgatt
    12541 gtataccaaa aaatcagggg tacgcagata cggcagataa cgaaaaacag tccctggatt
    12601 gatgattttg tgaactatct cattaagaaa aaataaatac ctgataaaat gaaaatggtt
    12661 tctgcatata acctggaaat gtagcaaccc aatacctctt ctgtactacc cccgtacaat
    12721 atattgtacg gggttttaat gaactcattc tttatatatg tttccagata atgttttaac
    12781 tccaccataa acatttttac aattaacatt accactcata gactcaacat ttcctgaaac
    12841 attttcacaa gaaacatccc ccgatgcagt tttaaccata ccagcattac cattaatgat
    12901 tatatttccc aactcggtat gtaatgaatc aacatgacca ttaacaataa cagtaatttc
    12961 aggaagagct acggctttcc catgaacgac accatcaata atgacttcat tatttaatat
    13021 ggaaataatt ttcccctttc cttcatacac aacaccattt acagataccg aacctgaccc
    13081 tagttttatt ttcatcgcaa tattcctgca tattccatta aaaaccgcta gttattattt
    13141 cttctatcat catgcaattt ctcatacaaa cctgactcat gaccaggcag cagagataca
    13201 ttctctatcc cgtaacgttc aagaactgac tgaaaaaact caagcgtcaa agatgatgga
    13261 tctttctcat aaatattcga atataccggc tcgtcaagtt ctggctcaat aacaagaaaa
    13321 aacctggaaa gcggggtatc ccagccaacc agaagttcat aactacgcga tgctgatttt
    13381 cctgttaaac gatactggct cataactccc tcctgaataa ttcacgctca aaaacaatca
    13441 tataatcata tgattacaaa atcaagggat tatatccttt tattattctt atgtttgtaa
    13501 agcatgttgc accaaacagg cacatgtgcc tatcatggta tgtaaaacaa gatcttttat
    13561 catggcagaa ggagaacaaa gtcatgcaag agggcatagc gatagttaaa gtcaataata
    13621 ttgaagtcgg ctcaatgcca atatcacaat atgaagaaat agttaaatca gtaaaaaaag
    13681 actggagaac tcatgtggct tcagttttca gttacattgg atttgcttgg agaataaccc
    13741 ttaaactctg gcattatttt gttcatggtt tcgcaatatt tattgcctct attatgtttt
    13801 attctattta tcatcaagca gaattaatac atttaataac atttctaaga aactccccct
    13861 ctgagaatat agctgaaatt attagagtaa taacatacta ctgtattgct ttgacggtta
    13921 ttgccgtagc tatcagcata gcggcaaaag ggattccgat ctttgtgagc gctaccgaaa
    13981 ttgccataaa taaaaagatc cgggaagtaa tggaggttcc gacagaaggg caggttagca
    14041 ttacgttcaa aaaagatggg gtttatagtg tcagataacc gtgccgcatt tataacgtta
    14101 ctgactgata acgggttaac ccaggcgcaa gccgcgaacc taatttgtga atacactcac
    14161 cgaccatgct ctgtaagaac tatccgttcc tggattaatg acccggataa accaagctcc
    14221 agaacctgtc ctgattgggc agtagaagca ctaattcagt ctctacaatc taggtagaac
    14281 acatctgacg tattctaggg ggctgaagtc caatggaacg aaaacgtacg ttagtgaagt
    14341 aactgtctga tatatcaaaa cataatggac atagaaaaac gccatcaaaa cggtgtcttt
    14401 ttaatcgaaa attggcactt aacggacggc attgtctact aatcgatttt taagacgata
    14461 attttcctct taccgtacgt tttcgtacgg ttggccctca aaccccttct actttacttg
    14521 gcataaaagg ggtttgggga gtagtggaac tcaaaaggcg gttaagcggt tcaattgaat
    14581 ggtcaataac aggtaaatca atctgttgag atcaacaacg tcttttgttc cgcaaacggc
    14641 caggatgagt aaagccaact tggcccccca aaacagatag ccaagaacgc agattcacga
    14701 tgtaaattgg ctggttgatt tttggctatt taaaatgacc gcttaacggc ggttattgtt
    14761 ccactgagcc tcaaacccct aaaacaaata attacggttt caactgctgc acaaagaacg
    14821 aaaccgtaag tctctgttcg aacgctcccg ctgctccaac tttaggcatt ttcccctccc
    14881 ctttgttcac atagtacctc tgccaaacat tctaataatt ccatactcat agaatcatgc
    14941 aatcataaaa tcacttaatt ttataattat cgaatcaagt gattgtatag ggattccagg
    15001 ccggaaacct ggatttttcc gcgaccagta aggggtcggt tagggaaacg tacaatattg
    15061 ggggcagttt ggagatcggg gttgtacgcc ggaaacgccg aaatttccgg gaggcacaaa
    15121 ttttatgctc ggcaacctgt acgtaagacc gccgttaacc ggacagcaaa gaaatagctg
    15181 tttagagcga aagaatcact gcccgtaacg tatcacatag ctggtacgga taaattctca
    15241 cttactgcac cataaacgta ctcgtcaaca accgccaaac atgcttcaaa aatcaacaaa
    15301 ttcgctgtat cggaaatttc ggcgtttccg gcgtataagg gggcggtttg ggaaacggaa
    15361 atttttgtac gttaagcgca ttatttaatc aatttaagat tctaacgtat tgatttaaca
    15421 tcagcggcta tcgcctttaa gttatcgctc ttgctatgtc gcatttaggt tatccgatta
    15481 aggccaaagc ccctgaaaac gccgtatagc acgggagtac acccaaccgc gatagcgcaa
    15541 ccgtacaata ttgttcgttt cccaaacgca gccccagaaa aaccaggtat ctctcgatcg
    15601 acgtacttca ggttaaaaag aacaataaaa aatgatgcta aaattgcaat gttttataaa
    15661 taaattcaat taattaccta agcaaggtga gacttttaga ggaaatgggc gcgacccctc
    15721 agagaactca ggccgggctt caagcagcac gagccagagg acgtaagggc ggcaggccga
    15781 aaacactgag taaggataaa caggcccttg ctgttcaact ctataacgag aaaaaacata
    15841 ccgttgcgca gatttgtgtg ttgatgggga tatcgaggcc aacgctgtac aaatacatag
    15901 agtcagccag gttgttcaag aaatgaactt agcgtacgtt ttcgtacggt tgggcttcaa
    15961 acccctatgt taatgctaat gtatgcgtac attagcatta acatactgcc ccgctaagtg
    16021 atttccgtga gggagaaatc acttagcggg tgttatcgca gaaattccca cggaacccct
    16081 agctcgctgg caatcgcgcc acgatcatta ccataaaata atatctctcg cagttaaaat
    16141 aattgaaccc tcagcactca gtgcccatag tataaaaaga gtacatctta aacttttatt
    16201 tttcgcaagt tctcctgcgt acaaccagat tgaagaagcc agccagcaaa gaataaatat
    16261 aaaggaaatc gacatggcgg aagctataat aatattaaaa ggctccgcta ttttatctaa
    16321 cagaaccgga gacattaatg caataacgac gagcgcaata ttacccagca gagtgtcatg
    16381 aatgattttt ctgataattt caaatttttc tcgattaata atcatagtga gtacatttcc
    16441 tgtaaagagt gattattaga gtgtgtttat caattatatt gataaatagc acagttcatt
    16501 ttgtaaaatt ctctatatcc aaactgccct caatataatt gaagcaataa gtaggctaat
    16561 tatcgtcgtt aacaacaaag ttattttctc tttagatttc aacgtgacga taaaattcaa
    16621 aatagcgaga gccaccacca ccacccactc aaaaaaatta tctgttatca gttccacatt
    16681 aaatcccttc tataatcaac ctcacaatca ccgcacatca gattagcgcc ccatataacg
    16741 attacgtaaa atatttatca ttccaacagc cagcataatg gaagctattg cacataataa
    16801 agcaggtact ataaacataa attcactttc cattgtgtcc agacctttta taagtgctgc
    16861 atgaaagtct gttctggtgg aaacatctgg agatgacata gtagtcacta cattattaaa
    16921 actgcttatc agaggcaaga gaactgctcc aataagcgtc agttttgcag gtaagcgaat
    16981 aaaatctttc ttagctttca tgatacgaca catcaggtat gcaactacaa gcatccagat
    17041 tgctacactt attgccttta tacaaaaaga cggtaattct cgcagaccat caatgtaaat
    17101 aactcccata gttcctagtc ccgcgacgat tgcaggaagg aggaataaag aaataacaac
    17161 aacaattaat gtaataacag gatttgattt tttatcacgt aaattattca taaataatat
    17221 ctcccacttg attacaataa ttctctatcc aaacactcat acacaacaat cttataatca
    17281 taggattata aaatcaagag attatttgat tctccttatt tttcaatcat ctgttcagaa
    17341 taacgagaag tagcggcaga gtgagagtgc tacgtgaaat cagcaaaaga gggagaccac
    17401 gtagcaacgt catttatcta cctatcgttc agcggaccat ccatcatttt tcaaaaccag
    17461 agatagacgg tttttctgtt tctctttcaa attaaactta agaagtggaa atgctgcctg
    17521 aatatcatcg ctctttgccc attcagcaat attcgtagga ctgaatgtgt aattgaccct
    17581 tgttattttc attcccatcg catcaccagg ttcagtaaaa tcaatgatct catcaacctt
    17641 ataatgacca atacagaaat cagggcgctc tggagatttc agatattttt tcccattctc
    17701 tgtcagagca tattcacgcc caggcacttt tttacctgtt ttagaaaatc cgatagtttc
    17761 atcaactagg gtatctttta ctgtcaataa accagcttta accagtgtat caaacatgga
    17821 aaactctttg gtatttattt cgtctgcctg ttcctgagat gtatatcgat tagtttgggc
    17881 gacagataat tgaactggat aaggcttact atcaaacctc ccaagaggac tgattgtaat
    17941 gcattccttc tcaagtttcg tattaatcgc tttttcaaag tttgctttat tggcatcctt
    18001 cgatgagtca caccctgcca acaataatga acttaacgca attatcacaa ttttttttga
    18061 attaaccatc atttttcctc tttaccagaa aaaaacagcc tatattttca gtatgtaaat
    18121 atatttatcc ctttaaatta ataatcatct aaatcaccgc aaccaagagg gcagcaatcg
    18181 cgatcaaggc cgtggcaaca atagcctttt tctgcgtctc ggcgtatttc atctgcaatc
    18241 tcttctgcag aaagataatc aaactcctgg cgagccagtt caaacgcctg agcatgttgt
    18301 tctttcggca atctggcgac aaattcatca atcgcagcgc tcaaacgacg ttgatacagc
    18361 tcaaaaacaa gaccacaacc aggcatgatc ttttttgata aaagatccag ttctgagcga
    18421 aatttatctg tatcgaaagt ctccataatt ttcacctgcg caagttattt gatgcgattg
    18481 ttgcccctga tttggacaag caactttcat tgatactgat tataaagtat acgctcatgt
    18541 aaattcttat ggaatatgcc gctttagcgg catggaaggc gcgaaaaatg gctttataga
    18601 gtcacagatg ccaggtcgcg ccgatacccc taaccaggga gtttcatgtc gccaggaacc
    18661 gcgtcagccc cctgccaagc ctgaccgcac ggaacctaca gctattcagg tggtaggtgg
    18721 tcgtagaccg tcgatgttac agcgaagata gatgaataat agttcgattg tgatgatggg
    18781 atgctgttgt ttctaatgcc ccgagcttgc gaggggtgct acttttgagg cgcttgcgcc
    18841 gatgttttta ggttattcac ttgttaaagg ggataacagt aatgtttttc ttttttgctt
    18901 ctcttttagt acgtaagtaa ctaaaagaga agcaaaagag taattgcggc atatcgtttg
    18961 cactttaagt atgattatcc acaacctgtg cattgtttac ccaatgatac agcttataac
    19021 tgctcttttc tgagcatgga aaacggaaaa taacccacgc caagactatt ttttgtacag
    19081 ctttttatta tggacgtggc gatacaccgc cccataaggc ggtaagttag cctgtatcag
    19141 accggaatag gggtaggtgg gtgagcgtaa tattcttttt tgcgttgtcg agctgcgtcg
    19201 cgtttcgctt tcatccgggc taatccggct gccgctttcc gtgtccagtt ctggactctg
    19261 atagctatca gtctggcatc cccggagtaa atcatttcat taggatctgc ctttaacagc
    19321 ctggcacgtt cccgataaac cagttttaac ggaatgccga gtatggcaaa gaagtcttcg
    19381 gttacttcaa tgtgtttaca ggcacaccgt gcatcatagg ggttaaacgg cgctttatct
    19441 gtaatgatcg cattcaacca gaccaggcga tcacatcccc tactggcacg ggaaatagat
    19501 ttacgccccg cgtcactgcg tgtcaccagt ccggcacgtt ctgccagtgt ttcgaggtta
    19561 tgcctggttt cccaggagcg taaatcgaga tcatccagga tagctaacat cagttcgcaa
    19621 agtgcctcta tgccaaactt gttagcctca ttaacccaat atcggttatg ccggatacgg
    19681 cggcggagtt cgtttttgat tttgacacgc ccagcgaccg tcgccagcga tttaatccag
    19741 cgattgagga aacggcgtcg cttacgcgac acaacgttat agcatggact ttgtttggcc
    19801 acccctgacg gcgatatgcg ggggattaag gaattgttct tttcagccac caccgcccgc
    19861 acttattgcc gggcagtagt cttgtattga aaaatgcatt tgctataatc ctttacaggc
    19921 aaaagcctgc tttggacccc ctgattatct tctttggccc cgcaaaagct ctaagagaga
    19981 tatcaggggg ttgttgcatt ccgggggcta taaattcgct accccctccc ctggacgctg
    20041 ttacgggaac gtcctaccct tataaattcg tcaaaaccgc ataaaaataa catgcgcaga
    20101 tgttaagatc aatctatgcg ccaaaagcca tcacaaagca tgttcatcat ttatctttaa
    20161 tattcataac attatataaa tattaaaact caaaaattca cgtgcaacat aacacaaaaa
    20221 gttaatgcta atgtacgcgt ccgttagcat taacaaaaga gcaatgacca tcatccgtca
    20281 aagatagcat tatcatagtc tggttaaaaa acagtcttag cgcggcttat tttccgtttt
    20341 caacgcccca actaaccctg accaacatag cggagatatt caacagaccc taaaatctca
    20401 aaactgtctg gatctgcctt acgccagtga ttttttgcac gggaagcata actgcgatcc
    20461 caacgaagaa tgcgcttacc atcctggata atacatacca tcctgttttc tggtgtccgg
    20521 tatgatttga taatgtgcac taaatcatga tcgggattaa aatcaatcaa ctcttcccga
    20581 ccgttatttg acacaacaag tatactgacc atcatctcaa tctccatttg tcttcttgca
    20641 gttctcaaac atcgagcgta agtgcaaaca tcaccctctg actcccttgc gcctcctcgc
    20701 ccactcgctc acctggcggt tcggtcgctg gggctgcggc gggcgctatc atctctctca
    20761 taaaagactc aatacctgaa aatcggaaga tcactaaagg caaaggatct gtctgcaagg
    20821 atcaagatcc ggtgctgtta tctgatatag gttgtttatt tggtctcatt tgccctacgt
    20881 cgataatagg ttgcccgact gatccctaac tctacccacg gtttcaaact ccgcgcagaa
    20941 tcctcaacaa tccctctttt agatattttc ccacctttac gccccttagc agcctgcact
    21001 tctgagcgat tcttacgccc tctacgagcc tgaatttctg atgtatgggt atcagccaca
    21061 tattgctcaa atccagcttc agtgaagcgt ttatgcgtcc atttggcgat gctggagcca
    21121 atgcatacac attctttgtg agacaaaggc acgcttaact gcacgttata catttcgaca
    21181 cgctgaatca ccgcttcttt ccattgcggg aaatcaggcc agccctgccg gattgctgta
    21241 tatgaccaga tacgggtcag attgaagagc caacaatttc gcccaagccc ataactaaag
    21301 tcgatatcga atgcctgttc atcgttagcc gctgttggcg cgtacaaatc aacataatca
    21361 gccagttcag ctaactcata aagttctcca cgccattcaa caacctgcca gtatgtatgg
    21421 agaggatttt tacaaatcag ccctgcatag ccggtatctg atttaagttt gtcacgtaat
    21481 cctcgctcta ccgcagcagc aaactcgatc gccttaatgc taccgtcttt ggcaacacga
    21541 accgccgttt ttaacccata aaacagatgt gcatgaccat taaccggatt tttgaccgta
    21601 aaattaggtg tgggcgcatt aagatcattc cagtcaatcg ccgcaactgg cctgtcaacg
    21661 tcaaaaacaa gccagtacat tgcatgaggc ggattatgct gtatataacg cgccagcact
    21721 gctttagctg ccttatcgat acgaagccca tagagcaatt cattcgcgtg ataaggtcgg
    21781 tatggcaagt ttgccgcaaa ataatcaatt gccggactaa tcatatggcc ctgcacttat
    21841 gcctgaatga cacattattt ttcttgccgt taccggatga tttaaataca atttaaatgg
    21901 gcttttatag tcccctgata atgcccacaa gctgactctg ccaagtatca acctcatggg
    21961 tattatcatc cccgtagtaa aaaccaaaaa cgccggggtc actcggaact tgcttctgag
    22021 tgactccggg gttgaacaag ccgaaggcgc gttagtcggt ttcattgctt tgctgcttgt
    22081 ccgtactgcc agcgccattt tcaggaatgt cgtttccggc ttttcctccg cttactttat
    22141 cctggcgact ccccagcatc tgcatttctc cccctgccgg attaacaata atttccgtgc
    22201 tgtaacgctc tatattgtct cggtccagcc acttacgggt ttgcagtttc ccgcgaatgt
    22261 acagcaacgt gccttttttt acatattgtt ccgttatctc ggctgttttt ccgaaaataa
    22321 caactcgatg ccactcggtt ttctcttttt cagcacctgt ctttctgtcc gtccatgttt
    22381 cagttgttgc aacggaaata accgcgatct tatccccgct actggcctga gatatcaccg
    22441 gagcattacc aacaaaacca accagggtga cttcatttac accttttctt gccactgttt
    22501 accccttctg ttaccagcgt aattttccgt atttatcaag tatcttgcct acaatatctc
    22561 caggtgctga cggacaatca ccaagaaacg ctttccgggc attaaatgtt cttcctggat
    22621 gaaaactgcc atgcttcctt ttgataatgc tgaaaaattt tttctttgcc ccactacatt
    22681 caccaggcga ttccccctta agcattccag ccatacagag aaaaacagta catggatcac
    22741 tactgttaac aggttgctca agaaattctc ctgtcagtga cagagcatca cttccatttt
    22801 ccgcgctggc gctatgcacg gttccaaatg agacaaaagc tgcaagtatc agtatcttta
    22861 tttttgtcat atttaacctc ctaacattgc catgatctta tttacatact gttgtgtttc
    22921 cttataaggg gggataccgc catatttatc aacagcacct tccccagcgt tatatgcagc
    22981 cagagcaagc cgtaaatccc cgtatttatc gagaagatat gaaagatgcc ctgtaccaat
    23041 tttcacattg gtttcaacat caaatggatc agcattatgc cttttggtat gtacaggcat
    23101 taactgcatc agtccctttg caccttttct gctgacagcg tttggattaa atccagactc
    23161 cgttttaatt aacgctttca acaaagtttc actaatttga tatttttctg cgtatttatt
    23221 gattatcgac tgtatttttt cagatatttt tattcctgtg gtcgattctt tgttttctgg
    23281 ccctgttttc tccaccaccg gctctgacac ggcattaatt tcagcaatca ccgcttctct
    23341 tatctcgcgc aaactacgca tatccttcat atcagttgta ctaccccaac gtccctgcat
    23401 cgctgtaatc cattccgaag aaggtgattc atcggtaaag ctggtaactc ctggcgcggt
    23461 tatcgacgcc agtagaattt cttcatccgt tgatgcgctg ataccgccag atatcagaaa
    23521 cgccagcatg aaaattaacc ggacgttcac ggctcaatcc ccggtacgct tatctgctgc
    23581 ttctccggca aattgctcat cgatgccgga acagtcagat cccggtaaat gatatggcgt
    23641 tctgacagtt cgaaaagctg atcatgacca ctctcataac gttgtgcaca ccccgtcata
    23701 aataagcaaa gaatcaccgt tgcaatagcc aggaaagcca caaaaataat ccgcatacac
    23761 cattccttca tacaccgccc ccatttttct gaaatgaaac cagcctgtgg gcacgatcaa
    23821 taacaacata attatcaagc ccaaccagca actgaagagc atcaactacg ggcattgtgc
    23881 gttgcattct ggcaattggc ggtaatggct tatcaacaag cagagaagcc tcacgcggtg
    23941 ccggatgact cgttaccagg cgatatccag ttggttccag gagatagttc accgctgctc
    24001 tgatagtcct gatgttatcg ggccaaactg tacgcatata aacactcaac ggatcgatat
    24061 actgccagcg aggatctttc tggttcctgt ccagtggttg taactcctga aagtttccct
    24121 gcacatacac atcctgctgt gctatctgaa caggcgcact ttctcgcatc ctgtctacct
    24181 ggaaagggct tcttgctgcc tgacaggaaa atgcaaagaa tgcagcaaaa aacaatattt
    24241 cagcccgtat aatccgtttc ataaatattt ctccacggct tcatgtatat ctcatcttcg
    24301 gtaaagaccc tgatcggtgc tcccgcatgt aacgttgttg taggcacaac ctggaggtat
    24361 ttttgagcga tatttcctgc ctgagtacga gcgccttcgc caaaattccc ggcaaaactc
    24421 tcgttactat cacctgagcc ttcgtagctg gttttcgtcc ccacaagggc ataggccgct
    24481 acgccaagaa actgagcgag caaatgcctg tccacctcat cgccttcaac cgccggagtc
    24541 cccattcggt caagagacga cgaggtttta aaatcgaccc tgttaccatc aggcaataca
    24601 agccatgtaa cctggaatcc gatacggctt tgaaggattt cgtttacctg ggaggcttta
    24661 acgaccctga taacgaactc acttccttta ggcgcaagaa tgtagctatg cgtcgcatca
    24721 tagatatcat gcgtaagcat ggccttcatt gagccgccgg aataatcaga aatagccgta
    24781 aatgtggtaa tggcattcag gaccgtacct actggcaact tcatcattcc atcaacactt
    24841 gccccgtaag gattactggc tgtatatccc acaacaccgg acggtacgct ctgggtactc
    24901 cccttagtta ccgatgtcgc gttacttcct gctccaccct gtcgggcttc tgcaaattgc
    24961 tgttccccac gtatccgggc ttcttccagt cgtccaggca aagcggcttc atcagcacga
    25021 acttttgatt ccatcatttg gatacgttcc tgcaatccat tctgctctgc gtttatatcg
    25081 ctgatacgct gcgcattagt ccggtttccg gtaaacgatg ctgccggggc atatgcatta
    25141 ccactacccg caaccgtatt tttagtgctg tcaaaatttg ttttatctga ccttgcacgc
    25201 agtgctcggt taatctcatc agccttaaag cgtgccctga tttcatcagg tgatcccgca
    25261 tcacttgctc tcgcccccct ctcctgagac gcctcttttt cccggctgta agggtgaaca
    25321 ggttttttat ctgtttcact attcggattt ccattactgg cactatcctt atttccaacc
    25381 gtattagcct gattaacgga ggcagaagga cgtgcaccac tgtttttcag tagcgcatta
    25441 aacttttccg ttgtatttgg cgcagtgctg gcagtttcct gttttgcatt ctcctttggt
    25501 tcttcctcgt tccttgaggc aatacttatc accatattga tgaaaaggat tatcaggatc
    25561 acaccaaaag taccaaaagc gtaatactgt atttttttac ggtaagtagc tggtttttct
    25621 tctactttct cctgacgttc ttccataatt aactctcccc atcagtcgca ttatccgcac
    25681 ggctgacttc cagctttacg ccatccaggg taagggtaaa tttgggatat atccctgaca
    25741 tatgataaat ggcatatgca tcgtcgtact tggtaggaat aaccgctggt tttcctccga
    25801 catctgcatg taccgcaagt aatccacgat tcggttttgc gatacggata taagtaaaac
    25861 gcccgtcatc ataaacatca gagatcatat tctttccgct aaaaccttta cctgtcgtcc
    25921 agttataacg ggtataaatg aaatatcgat atcgacgaag agcttcaaga actgcgtttt
    25981 tgacttcgct ttctctgtta ctccgcaatg tattcacttc attctgtaaa cgagctacat
    26041 cagccccgga tgcccccata ctgtaagccg tgtttaatgc acttctgtct gcggaacgca
    26101 tacctgcacc acggatctga acacactgtt gattctggtt ctgagatacc cgaacggcct
    26161 caatatccag cgtctgcccg ttagtcataa atactgttac catcgttttc agcccttccg
    26221 gctcttcact ggtaggctga atcatgacct gatttgcggc tcccgtgctg gcccatagcg
    26281 cattagaaga aaagggttct ttcgttaata acattgagcc ttccggcagt tctatacgag
    26341 ttcccagata taaagcggac tgcactttaa ctattgttcc aggccgccag ttaaccaccc
    26401 tacattgctg tgctgttgca ctgaacgtag tcatgacaac agccaggact ccagaaaata
    26461 agagagttct tttcgctaac atgattaagt tcctttactg ttgtacggat ttatcatcaa
    26521 taatctggat tcccagcgga ttgatgttaa gccagtccag cgattgttta ctaagttcgt
    26581 tacgttgtaa caacgtccat tgaagacgcc tcatttgttg aaccgatgaa atttctttac
    26641 cctgataatc ccttgttatc tcctgataag taacaagcgt tcgaattaca ggtttgacat
    26701 caccatcaaa ctgaccttct atcgcgtcat aatggtcaat atcttttatt tctatatcaa
    26761 tcctttttgc tgccatatcg gcctgaatat ccacaacttt ctgagcgaca ttaaaccctt
    26821 tgggatcggc aaactcccct gtaagtacag gagacataaa tactgccgct tctgcataat
    26881 cccgttctat agtctctttt aactgtccat agcgcttctg cacatacttt gacagcaatg
    26941 agttcactgt tggtgtatat ataggctgct cattgtctgg cgtaaattgt gatattttat
    27001 gtgaaccatc aggcaataat ttcacccata ctacttctgt agtattctgg cttttctcta
    27061 ttgcctcctg aacagaatat gactgatata tcgtcgtccc ggcaagaagc aaggcaatcc
    27121 agaaaaacaa atttctgtct ttttttagtt caccaacacg atccttaaag gctgtgcgat
    27181 ttactttttc gacttctttt ttttcaagca gcatacgttc accttacaat aatgctttag
    27241 ccgctttctt ggctagtttt actgccatat tattcaaacc ctgacttgca gagccgacac
    27301 cagaaccaat catgtacgca aatataaaac aactgaacac cataattgca gcgattacag
    27361 aatatgcaca aatatcacgt aacaagctga tattattagc tgttatatta agaacgccat
    27421 taacagtacc atctgacgcg ataacatcac cagcgccctt gaaactaccc gcagagttaa
    27481 ttgatgccct gatcattatc attaccacaa tagatgttat tttaagtaca accgctgcaa
    27541 gcccccatcc aatatagaat ttaaaccatc catcaaaaat tcctcttgtt gctggcgcaa
    27601 ccaaaaaagg aataaacatg acacctgtaa tttttgctat tgctgtccca acatcaatcc
    27661 aaacatcagc aagatatata gcaacagaca aaatagcaga acatatttcc cacaagagcg
    27721 ccatgccaac catattcatg ccatcaaaaa tatcaaccga tggtgctgaa atagcctttg
    27781 ttaacacgcc ctctacaaaa ttttgcagaa aactcggctt aatattacct aacgccagtt
    27841 tctgtagtgt atttgatatc gcataaaaag cgcctgtgat agtatcaaca accattgagt
    27901 aagaagcaag caatgacatt gataacataa acatgattgc aagcgtgaat aaatcaacta
    27961 acgccccacc attttccacg aaccgccaga aataaacgaa tgccgttacc gcagccagga
    28021 aagttgatgc ggcaatagtc cagacacgta aatcactatc cgggctattt agtattttat
    28081 taaccatctc acttacgcca ttctgaaagt ttgtgcgcat agctgtttct gcactggcat
    28141 aaaatgagac agataaaaac aggactacaa ataagagtct gttaattaaa gacaaaaaga
    28201 atcctcgaag cataatagcc cccaaattaa agatatagcc tgttaaacgc tacatcattt
    28261 acctatttgt ctgagtaaca gttccgcaca aatcttatca catggattaa tccatgctgg
    28321 ccagtgtttt ctaactatat agttaatctc cctattaata gttagtgtat ttaattttcc
    28381 agcccaaatt tctggatttc gttcgtcctt ttctacaact ttataaagtt ttatcaattc
    28441 atgcaatttg gctgcggatt gttctttggt cagtccatca taatgctcat gattatattt
    28501 atctcgccct ctaaactcat cctcatattc aaaatgagcc ttatcccatg cctgagactt
    28561 acacatatta ctattgcgtt ttccttcgca ttttttttca tatttagaaa tcagagcatc
    28621 aatttttaga tcaacttctt tttggtgttc atcaacatag ccagtctttg ccatagccga
    28681 agcggaaaac ataataaaga aaacaatcaa agactttttc atcattgcca tgccctctgt
    28741 aactgacgtg cctttaactc ttcctgttgc gctctcttgt taaaataatg ctcatcaaga
    28801 cgaccactat atttcatcgc ttcagcttgc tgtaactgta agctttgaat ctgttttaac
    28861 agttgttcat tttgttcagc taaaaactgg atagatgctg tcgtatcgtt atcacctaat
    28921 cttaagcgcc ttttctgtag ctcttgcaaa gtatctttct gttcatcaag ttgaatctgc
    28981 aacgttccta caaaagcatc ttttgaaatc ccttcaacaa cccgacgttg ccgatattca
    29041 aacatctgcc gggcacgttc acgatcatca ctcgtatatg gtattgctgc cagcatttcg
    29101 gcatattctt cgtccgtaag tccatagcca ttgagcaaca aattacgctc ataggcatta
    29161 tcccacggct cattacccgt atttttaacc atacgccccc caccataaac gggatcgaac
    29221 tggttagaga taagggctat ttcatccata aaattcttat agtcaatcga tgaaagttta
    29281 tctttcagtc cttcggcctc acgtaaaatt tgcttataat cctcaagcat ctggttgtat
    29341 tgcttatatg cttccagcaa ttctttatta ttgagaattg tctgttccat atattcttta
    29401 tagtttttga tatcctgcat catagccctg gcctgaccaa gaatctgagc agcatcagca
    29461 acaggaatgc cagaagaaat agcaggtgtt gtaatcaatc ctgtcattac agaaaacaag
    29521 attactgcaa gttgttttct cataagaaaa acccctccgt tttatccttt tcagtctctg
    29581 taggcgttgg gatcaagtcc ttattacact tcgttatcag cccttctaca atctgtcgca
    29641 ctgcaacatc gggatctgat tcacgtttcc atatttccgc tgccagggcg gcttcattag
    29701 acgttgatgt acaaacagca tactgttcag cctcaacaaa cagattcacc acttttgcaa
    29761 tctgcatgtc cgggatcttt atatacgact gctgttttac cttaaggctg cgaataatat
    29821 tcacttcttc ttcatcgaag ccataagcat catggaatgc tttaactgtt gcctcattat
    29881 ccggcataaa gataaacacc gacgccgagg aacgtaacgt agtccactca tccagtgatg
    29941 aatagtgctc agggttctga gtccagaatc ccataccccc accatgctta aaccaggttc
    30001 gggctttctg tgtagcgaac tctgcggcat gtggtacaga cagggtgtac tgtgcctcat
    30061 ctatttcaaa aaactttggt atcgtgcgat attccggtga ttcaaacaaa cgaacgacac
    30121 gaaagaagat ttcacggtgc acaagccgcg caagattatg gttatctttc acacctgaaa
    30181 ggttgtaaac agctaccggc ttatcaagaa caccaatcgc gtcctctttg ttatcaaata
    30241 aacgtccata aataccatta ctaataaatg gcgctaactt gcgtttaacg ctctgaccac
    30301 acttagaaac cagcgttgac aacgtgattg agcttaaacg cccctcattc ggcgaaaatt
    30361 tttgtacaag aagatcactc gcacacactg ttatattttc aagttcatca ggcgttaaat
    30421 atttatcctg ctcactgtca ttttctgctg ccattgcttt taactgctct ataagatgcg
    30481 caagaaatac cctgtcatcc ttgctttgcg ccatactgaa catgttaaaa ccacactgaa
    30541 aatcgctatc aagggtaaat gcagccccat catccccaaa gaaattagcc attgggattg
    30601 agccaggatc gatatccaga cagttataca gaccaccgaa tttaataaaa tggctggcaa
    30661 tacagttttt cataaaagtc ttgccgccac gggtcgcccc attaccaaga agcagtagct
    30721 tttctttaac ttttggcgta aagaaaaacg gcacgccatc atctgattca agaatataga
    30781 aggcttcctc catgcgtcca tttagctccc actcaggaat acctggatgg gatttaaaga
    30841 acaaggatgc agcccccatc tgagatgcgt taagctgtaa tgcacgaaat gtattgcgtg
    30901 agtaggctgg ttgcataacc ttgtaagcat ccagttgtgc taccgtctcc catacaactt
    30961 gcatggtctg agagacacta cggttaatat ccgcacaagt ctcaagtaat ttatcgggat
    31021 ctgtattaaa tacagcaatc ccggcctggc agatccccag gcgctcatct gaatatgaaa
    31081 tattctccag ttcttcgcgc atcttcatca gatgtgaatt tctgcgcaat ttttcatcga
    31141 gaatatcttt gctcgtactg tcagtcatta aatcgctcaa actgatttga gtcctgacaa
    31201 gttcgttttc tttcccgctg atcatcagtg acttcttaaa ggcactggcg ggagtgaacc
    31261 gcatgaagta aacgtagtta ccccgctgta aaactggtct gccatttgtc agcgcccagg
    31321 cacattcagg tacagattca tcgccaattt tcaacacact ggccaggcga acataaacag
    31381 gccgtacccc ctggatcttc aacatatcaa cgccacgcca ggtaatggcc tcgatatcgt
    31441 caccttcaag cgccagacaa tcccacatat cattgggtac agtgacctta cggcgtggag
    31501 ccagccaatg gtgattaaat gttgcaagga acttctgtaa tcgccagatc ccttctgaat
    31561 caagaatcct gttatcttct gaaataaaag aaaggcgtaa gtccagatcg cgtaatgttt
    31621 ctttcagcaa caggcactgt ttttcaaatg cctgacgctc aatgataaac gattcgcgat
    31681 ttgacagtgc cagcctgact ttacgtctgg ctgtgtcatc aaacatgctg ttaaaaagat
    31741 tctttaaaaa ctcggcagat gccaggcgat taatattctc ttccgggtga acatccagcg
    31801 tccatataat tcttgaggca tttaaattac gacatttatt aagatatgcc tggcgacgca
    31861 tagaaagtaa ctgactgcgt tgattatcac gccctgtcag ccggatctta acgccgtcat
    31921 tatggatata atactgagta agtgtgacag aaaggggcag taactgaacg atattacgaa
    31981 gtaataaaga aatacggcta ccctgagaag gggtaacacc cgttggatca acacctgtca
    32041 gttcaatagc accaaaagca cctccagtat aggtatacac ctgcccggaa tgttgttcga
    32101 tgcctgccag attcagatgt tcgttatagc tctgccccat cgtggatcac ctcatttttg
    32161 cagccaggca ataaagaacg ccaggttaaa cgctgcaatt gctgcaacag cagcacgaaa
    32221 cagtttcccg atagcctggc tctgttgcgg tgcaagtccc cacaacacaa tacaagcaaa
    32281 cccactgaca aacgcaaaga acaaagctcc agccccacca accagatcca gaatactttg
    32341 catccagtta acgattttct gtaaaaaact atcatccggc gtaggaagtt taactgtact
    32401 attaccagca gccatagccg gtgtgacact tcctgtcact aatgctacgt aaaacattat
    32461 ctggaatgtt gatatctttc tggaaatgcg attgagcatt gtatacacct cttttactgg
    32521 ttaaatccct aaatagcgtc tataaatgtg atacgacgct tcactgcgtg ggtattgttc
    32581 agcctggaag gtgacaaata accataaatc caggctttat gagcatcggg atcgccatga
    32641 aaaacgaact tcataggaac aaccgatacc agaacaaata aaatcagcga aataacggaa
    32701 aatgtgctga aatacataac acacagcaca acagcaaaaa cattcaggaa catcaggatc
    32761 aaaaagacct ctgtaggtat ccccagcttt gttcgtctgt tgtaaaaaac ttttgcatta
    32821 accagtgctg ggttggttat ttttcctttt atttcagcca tttgatttct taccgaatga
    32881 cgtagtatat ttttacgttt tgtaaaattt atatt
//
LOCUS       DAZGCQ010000052        32707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000052 DAZGCQ010000000
VERSION     DAZGCQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.052"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(155..901)
                     /gene="cutC"
                     /locus_tag="ACHK07_002667"
     CDS             complement(155..901)
                     /gene="cutC"
                     /locus_tag="ACHK07_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO8102981.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(915..1481)
                     /gene="yecM"
                     /locus_tag="ACHK07_002668"
     CDS             complement(915..1481)
                     /gene="yecM"
                     /locus_tag="ACHK07_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8102982.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1697..3430
                     /gene="argS"
                     /locus_tag="ACHK07_002669"
     CDS             1697..3430
                     /gene="argS"
                     /locus_tag="ACHK07_002669"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO8102983.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLATAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3472..3516)
                     /locus_tag="ACHK07_002670"
     CDS             complement(3472..3516)
                     /locus_tag="ACHK07_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8102984.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3607..4095
                     /gene="yecT"
                     /locus_tag="ACHK07_002671"
     CDS             3607..4095
                     /gene="yecT"
                     /locus_tag="ACHK07_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO8102985.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4217..4609)
                     /gene="flhe"
                     /locus_tag="ACHK07_002672"
     CDS             complement(4217..4609)
                     /gene="flhe"
                     /locus_tag="ACHK07_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO8102986.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4609..6687)
                     /gene="flhA"
                     /locus_tag="ACHK07_002673"
     CDS             complement(4609..6687)
                     /gene="flhA"
                     /locus_tag="ACHK07_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO8102987.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6680..7828)
                     /gene="flhB"
                     /locus_tag="ACHK07_002674"
     CDS             complement(6680..7828)
                     /gene="flhB"
                     /locus_tag="ACHK07_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO8102988.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8030..8674)
                     /gene="cheZ"
                     /locus_tag="ACHK07_002675"
     CDS             complement(8030..8674)
                     /gene="cheZ"
                     /locus_tag="ACHK07_002675"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO8102989.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8685..9074)
                     /gene="cheY"
                     /locus_tag="ACHK07_002676"
     CDS             complement(8685..9074)
                     /gene="cheY"
                     /locus_tag="ACHK07_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO8102990.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9089..10138)
                     /gene="cheB"
                     /locus_tag="ACHK07_002677"
     CDS             complement(9089..10138)
                     /gene="cheB"
                     /locus_tag="ACHK07_002677"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO8102991.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10141..11001)
                     /gene="cheR"
                     /locus_tag="ACHK07_002678"
     CDS             complement(10141..11001)
                     /gene="cheR"
                     /locus_tag="ACHK07_002678"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO8102992.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSREWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11020..12621)
                     /gene="tap"
                     /locus_tag="ACHK07_002679"
     CDS             complement(11020..12621)
                     /gene="tap"
                     /locus_tag="ACHK07_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO8102993.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQVEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12667..14328)
                     /gene="tar"
                     /locus_tag="ACHK07_002680"
     CDS             complement(12667..14328)
                     /gene="tar"
                     /locus_tag="ACHK07_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO8102994.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQSPAQPRLRIAEQDPNWETF"
     gene            complement(14471..14974)
                     /gene="cheW"
                     /locus_tag="ACHK07_002681"
     CDS             complement(14471..14974)
                     /gene="cheW"
                     /locus_tag="ACHK07_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO8102995.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14995..16959)
                     /gene="cheA"
                     /locus_tag="ACHK07_002682"
     CDS             complement(14995..16959)
                     /gene="cheA"
                     /locus_tag="ACHK07_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO8102996.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFGVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16964..17890)
                     /gene="motB"
                     /locus_tag="ACHK07_002683"
     CDS             complement(16964..17890)
                     /gene="motB"
                     /locus_tag="ACHK07_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO8102997.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGNRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17887..18774)
                     /gene="motA"
                     /locus_tag="ACHK07_002684"
     CDS             complement(17887..18774)
                     /gene="motA"
                     /locus_tag="ACHK07_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO8102998.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18901..19479)
                     /gene="flhC"
                     /locus_tag="ACHK07_002685"
     CDS             complement(18901..19479)
                     /gene="flhC"
                     /locus_tag="ACHK07_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO8102999.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19482..19832)
                     /gene="flhD"
                     /locus_tag="ACHK07_002686"
     CDS             complement(19482..19832)
                     /gene="flhD"
                     /locus_tag="ACHK07_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO8103000.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20612..21040
                     /gene="uspC"
                     /locus_tag="ACHK07_002687"
     CDS             20612..21040
                     /gene="uspC"
                     /locus_tag="ACHK07_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO8103001.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGN"
     gene            complement(21047..22471)
                     /gene="otsA"
                     /locus_tag="ACHK07_002688"
     CDS             complement(21047..22471)
                     /gene="otsA"
                     /locus_tag="ACHK07_002688"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO8103002.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22446..23246)
                     /gene="otsB"
                     /locus_tag="ACHK07_002689"
     CDS             complement(22446..23246)
                     /gene="otsB"
                     /locus_tag="ACHK07_002689"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO8103003.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23413..24399)
                     /gene="araH"
                     /locus_tag="ACHK07_002690"
     CDS             complement(23413..24399)
                     /gene="araH"
                     /locus_tag="ACHK07_002690"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO8103004.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24414..25928)
                     /gene="araG"
                     /locus_tag="ACHK07_002691"
     CDS             complement(24414..25928)
                     /gene="araG"
                     /locus_tag="ACHK07_002691"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO8103005.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25998..26987)
                     /gene="araF"
                     /locus_tag="ACHK07_002692"
     CDS             complement(25998..26987)
                     /gene="araF"
                     /locus_tag="ACHK07_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO8103006.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27784..28287
                     /gene="ftnB"
                     /locus_tag="ACHK07_002693"
     CDS             27784..28287
                     /gene="ftnB"
                     /locus_tag="ACHK07_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO8103007.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28366..28617)
                     /gene="yecJ"
                     /locus_tag="ACHK07_002694"
     CDS             complement(28366..28617)
                     /gene="yecJ"
                     /locus_tag="ACHK07_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO8103008.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28732..28818)
                     /gene="azuC"
                     /locus_tag="ACHK07_002695"
     CDS             complement(28732..28818)
                     /gene="azuC"
                     /locus_tag="ACHK07_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO8103009.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29081..29404
                     /gene="yecR"
                     /locus_tag="ACHK07_002696"
     CDS             29081..29404
                     /gene="yecR"
                     /locus_tag="ACHK07_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO8103010.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            29575..30072
                     /gene="ftnA"
                     /locus_tag="ACHK07_002697"
     CDS             29575..30072
                     /gene="ftnA"
                     /locus_tag="ACHK07_002697"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO8103011.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30110..30349)
                     /gene="yecH"
                     /locus_tag="ACHK07_002698"
     CDS             complement(30110..30349)
                     /gene="yecH"
                     /locus_tag="ACHK07_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO8103012.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            30540..31751
                     /gene="tyrP"
                     /locus_tag="ACHK07_002699"
     CDS             30540..31751
                     /gene="tyrP"
                     /locus_tag="ACHK07_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO8103013.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(31813..32478)
                     /gene="yecA"
                     /locus_tag="ACHK07_002700"
     CDS             complement(31813..32478)
                     /gene="yecA"
                     /locus_tag="ACHK07_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO8103014.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAYPQEQAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT         8021 a         8697 c         7790 g         8199 t
ORIGIN      
        1 tagcacaacg ctatgtgcac tgtcacccgc tcaacagatc gcggtaatcg ttcatttttt
       61 atactattca gggataaaaa aaggcccctg ttgaaattgc aggggcctgg tacgagcaag
      121 catcatattg ggcgacatga tgcaacggta aaaatcattt ggcctgatgg cgttcaatga
      181 ttcctttcat ttcagcaacc gccgccccgt ctacgacata acgcgaatac tcgtccgcgt
      241 gtgcatctga tgacatggac aatccttgat tacgataacg catcggtgag gcttgccacg
      301 ctcccgcgga gctatggact tccagcactc cggcatcgag gaagtggtgc aggttttctg
      361 cacggactcc tgctccggcc ataatgattg gagcatcacg atgggcaata agttccataa
      421 tttttgataa accttgcagt gcgtctgatt tttgccctga tgtcagcacc cgggcaatgc
      481 ccaattccgc aagattattg agtgtattta gagggttagc gcacatatca aaggcgcgat
      541 gaaaagtcac tgccagcgga ccggcagcag ccattatttt ttccattcgt ggcatatcga
      601 cattcccgtc aacatcgaga acgcccgtca ccagtccagg aaaacctaat tcgcggaccg
      661 tgcgcacatc ctcaagaatg gcggcaaact caccgtcgct gtagcaaaaa tcaccaccgc
      721 gtgggcgaat tatcggatgc acagggatcg tcacccgctg gcgcacggat ttcagtacac
      781 ccaacgacgg cgttaagccc ccctcttttg gggctgcgca taattcaact ctgtctgcgc
      841 cgttttgctg cgccgttagt gcacattcca tgctgtaaca gcaaatttcc agtaatgcca
      901 tttttactcc ttaattacgc cgactgctcg ctggcaacga tctcttcaat ggaccacgga
      961 tgaaacttaa tagttgtttt gccatcggtg accgccagcg tcggattcgg taatcgctca
     1021 tgctcacctt ttggcgaact ggttttcact gagatccccg gtaggctgag tccttcatca
     1081 gaaagcaatg ccagcgcacg ggcattcagc gtttctggat cacccggcaa aacgatttca
     1141 atatgttccc aaccttcgtg tgggtaacgt ttttccccgg gccacggtag ctccacaata
     1201 gaaaactgcc agtgcgcaac ctgtaccggt tcatgcaatt taaacagaca aatcggtctg
     1261 ccattgatca tattttctga caaaagctcg ccgcactgtt caaacccgcg acgccagcgt
     1321 tcagcggtgg cgttttgatg gcaacgcaag gaaatgtgat cggcagtcaa cggagtgata
     1381 tccaacccca aacggcggga aagttcatct aatgcgtggg taaatcgcgg taaatccgat
     1441 gcaatatcct gcagctcgtc gatagattgc cagttcgcca taatcactct tcgtctttca
     1501 gtaaaagcgt taatttaccc tgttgccctg cgccaaccaa ccgctgattt cacgccgctt
     1561 ctgatgcaat agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa aacagccatt
     1621 tgcagtatac tcccgcccta atttctttaa ctggtgcggg caatttttgc tcgcttcatc
     1681 aatgtaaggt attccggtga atattcaggc tcttctctca gaaaaagtcc gtcaggccat
     1741 gattgcggca ggcgcgcctg cggattgcga accgcaggtt cgtcagtcag caaaagttca
     1801 gttcggcgac tatcaggcta acggcatgat ggcagttgct aaaaaactgg gtatggcacc
     1861 gcgacaatta gcagagcagg tgctgactca tctggatctt aacggtatcg ccagcaaagt
     1921 tgagatcgcc ggtccaggct ttatcaacat tttccttgat ccggcattcc tggctgaaca
     1981 tgttcagcag gcgctggcgt ccgatcgtct cggtgttgct acgccagaaa aacagaccat
     2041 tgtggttgac tactctgcgc caaacgtggc gaaagagatg cacgtcggtc acctgcgctc
     2101 taccattatt ggtgacgcag cagtgcgtac tctggagttc ctcggtcaca aagtgattcg
     2161 cgcaaaccac gtcggcgact ggggcactca gttcggtatg ttgattgcat ggctggaaaa
     2221 gcagcagcag gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt tctaccgcga
     2281 tgcgaaaaag cattacgacg aagatgaaga gtttgccgag cgtgcacgta actacgtggt
     2341 aaaactgcaa agcggtgacg aatatttccg cgagatgtgg cgcaaattgg tcgacatcac
     2401 catgacgcag aaccagatca cttacgatcg tctcaacgtg acgctgaccc gtgatgacgt
     2461 gatgggcgaa agcctctaca acccaatgct gccaggaatt gtggcggatc tcaaagccaa
     2521 aggtctggca gtagaaagcg aaggggcgac cgtcgtattc cttgatgagt ttaaaaacaa
     2581 ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat ggcggctatc tctacaccac
     2641 cactgatatc gcctgtgcga aatatcgtta tgaaacactg catgccgatc gcgtgctgta
     2701 ttacatcgac tcccgtcagc atcaacacct gatgcaggca tgggcgatcg tccgtaaagc
     2761 aggctatgta ccggaatccg taccgctgga acaccacatg ttcggcatga tgctgggtaa
     2821 agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg aaactggccg atctgctgga
     2881 tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag aacccggata tgccagccga
     2941 cgagctggaa aaactggcta acgcggttgg tattggtgcg gtgaaatatg cggatctctc
     3001 caaaaaccgc accacggact acatcttcga ctgggacaac atgctggcgt ttgagggtaa
     3061 taccgcgcca tacatgcagt atgcatacac gcgtgtattg tccgtgttcc gtaaagcaga
     3121 aattgacgaa gagcaactgg ctacagctcc ggttatcatc cgtgaagatc gtgaagcgca
     3181 actggcagct cgcctgctgc agtttgaaga aaccctcacc gtggttgccc gtgaaggcac
     3241 gccgcatgta atgtgtgctt acctgtacga tctggccggt ctgttctctg gcttctacga
     3301 gcactgcccg atcctcagcg cagaaaacga agaagtgcgt aacagccgtc taaaactggc
     3361 acaactgacg gcgaagacgc tgaagctggg tctggatacg ctgggtattg agactgtaga
     3421 gcgtatgtaa tcgatttttc gtgagagtga agcctgatca gggttagccg atcaggcttt
     3481 tttattgcca tctaaatgta ttctgaaaac ggacatgcca tcgttttctc actgttgagt
     3541 aagaggccag aagcgtaata tccaggccca ggaaaacgat aacggttcaa tttaaggaat
     3601 accgcagtgt ttaaatttct tgtattaaca ttaggcatta tctcttgcca ggcttacgca
     3661 gaagatacgg ttatagtaaa tgatcatgac atttcagcca tcaaagattg ttggcaaaaa
     3721 aattcagatg atgatactga cattaacgtg atcaaatcat gtctgcgaca agaatacaat
     3781 ctcgtcgatg cgcaattaaa taaagcttat ggtgaagctt atcgttatat agaacaagtg
     3841 ccacgcacgg gtgcaaaaaa acctgatacc gaacaactta acttgcttaa aaaatcacaa
     3901 cgagcctggc tggattttag ggacaaagaa tgtgaattaa tcctttcaaa tgaggatgtt
     3961 caggatttaa gtgaccctta ttctgaatca gaatggctat catgtatgat catacagacc
     4021 aatacgcgaa ctcgccagtt gcagctatac cgtaactctg aagattttta tccaagccct
     4081 ttgacaagag gataattcac atctttttgg catgttttgt tgcaagctat tcctgataaa
     4141 taattgcaac aaaacatcga gcctttttcg ctgagttatt aaacatgctc gcgagcgcgt
     4201 aatttttatt tgtccttcag cgataattca caatcacttc attccgttgt acctttagcg
     4261 gtggaattaa ccgcccaccg ccaggcactt cccagataaa gcgcaacggt tctgctgccg
     4321 ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca caacgagatt
     4381 gcgaacacaa gcgaacccgc agtcctgcag gtgtcgggcc gataagctga taccgccacg
     4441 ccaccagcgt catcaatcct gaagcaggtt gtcgagtaga aagaggcgct gacgacatcg
     4501 actcaccgcg atgatttagc gtaattccga cactacttgc ctgccacatc ccctccccgg
     4561 cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatc atttaccgcc
     4621 aattgtcgcc gtcatgcgga tatgtcggtt atcagatagt tccagattcg acagcaccac
     4681 taactgcggc aagctgcggc gcagaaagcg agacaataat ggtcgcagcg cgtggttcac
     4741 caacaatact ggcggcgccc ccaacatctc ctgacgggat agcgcttcct gagtttgcgc
     4801 cagtaaacga tccgccagcc ctggctccag tccgcccccg ccctgcaacg cctgtagcaa
     4861 caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca
     4921 ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc
     4981 actttggatg ggtgcatgtt ccgccagcgt ttcgagaata gtgcgcatat cgcgaatcgg
     5041 cactttttca tcgagcagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc
     5101 aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca gcagctgttg
     5161 cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt
     5221 tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag
     5281 cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg
     5341 taacgtcccg gcagcggtac cagggttaat tgccagccag cgccccggat aagcatcacc
     5401 actgccaatc tctacgcctt tcatcaaaat acgataacgt gccggttgca gatccatatt
     5461 gtcgcgaatg tgcaccaccg gcggcaagaa gcccatttcc tgagcaaatt tcttacgaat
     5521 actacgaata cggcccagca actcgccatc ttgttggaaa tcaaccatcg ggatcagtcg
     5581 ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac
     5641 aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt tttgttcgcg
     5701 tccgcgtatc caccaggcca gcccgagcaa tccggcagtg aacagcaaaa ataccaggtt
     5761 cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct
     5821 tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     5881 cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc
     5941 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg
     6001 cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     6061 ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg
     6121 agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccgg cgttaaggtc
     6181 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc
     6241 tgcgatacgt ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacgaa
     6301 caccacgata ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac
     6361 cttcccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac
     6421 gttaagtgcc agacgcaaca gcgtggtaaa caacagaatg gtcggaaacg cagcaaactc
     6481 aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     6541 ggtaaacagc aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa
     6601 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgtgggcag
     6661 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     6721 ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt
     6781 tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttgc cccgggattt
     6841 gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg
     6901 ggacgttatt ttcagcgccg atttcacgaa tgcgcagcgc gacaagccct gcacctttag
     6961 cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg
     7021 tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg
     7081 cagctcgctg catctgacgg atccgtcctt taacatgggg gtcgccttcg ctttgtttga
     7141 actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg aagatttgga
     7201 aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc
     7261 ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccatt aagcgcatca
     7321 tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg atggttttca
     7381 aaattgcttt aagcaactcc gcgccagtct gtgccgagaa catccgtttt atgcccggta
     7441 gcgggttgag tttggaaaac ttcggctgca aggattttcc gctaaatacc agccccccca
     7501 gcataaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggt agcagcgcca
     7561 gcatggcttc tctgatcagc agaataattt gcccgaggat cagattcggg tcattgataa
     7621 tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca
     7681 caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag gtcagttcac
     7741 gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt
     7801 ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa
     7861 atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta
     7921 gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct
     7981 tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat caaaatccaa
     8041 gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct ttgctggtat
     8101 cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg
     8161 ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca
     8221 tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca
     8281 gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaatttc cgcgtatctg
     8341 ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt
     8401 gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac
     8461 tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc
     8521 gcgcatccgg gatggcttcc gccgcttcgg caatggcctg atccagcccc agttcccgca
     8581 aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat
     8641 gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc
     8701 tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag
     8761 ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc
     8821 attaacactg gcaatgctga catcgcgcca tccgcacgaa ttgttttcag caattccagg
     8881 ccatccatgt tgggcatgtt ccagtcggaa ataacaaatc cataaccgcc tgcctgcaac
     8941 ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc cagctctttc
     9001 agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt
     9061 tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattt
     9121 ttgccagcat ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat
     9181 tgatggcctc acgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc
     9241 atgcccccgc ctgacgcatc gccaacattc ctgccgcgcc gtcgttgccc ataccggtca
     9301 ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta
     9361 ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttga taatttgcgc
     9421 cactacgcgc cagctccata tgccgatcgc ccggcgcgat ataggcatgc cccggcaaga
     9481 cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg
     9541 caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc gggctggaaa
     9601 gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg
     9661 caatcagttt ttcagaactc aacaacggcc ctgccttcag cgttgtcggt gccgacaatg
     9721 gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca atcatttcgc
     9781 tgtacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctattgccc
     9841 ccagctccag cgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa gaaaccatca
     9901 caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg
     9961 gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcga
    10021 ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg
    10081 tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg
    10141 ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcaaggtg
    10201 gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa
    10261 gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt tacgacagaa
    10321 gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca gcggggcaaa
    10381 atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc
    10441 tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata
    10501 gataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca
    10561 gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc
    10621 gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac gtgcgtgatc
    10681 cgcgagcaga gggaaatgat gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga
    10741 attgataaac gcctgccact cacggctgtg ctgattagat tccagcaagt tcaagtaatg
    10801 accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc
    10861 gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg
    10921 aaaatgtgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc
    10981 acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat
    11041 ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac
    11101 acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc
    11161 taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg
    11221 ctcaatcccc cgccgttgtt cttccgacgc cgaagcaatt tctcccataa tgtcgttcac
    11281 gcgggtcacc gaactgacaa tatcggtcat ggtcgcggcg gcgttattca ccagtttcga
    11341 accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg
    11401 cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg
    11461 ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc
    11521 aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg
    11581 catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcagcatt
    11641 ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa
    11701 ttgctccata ctggcagccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga
    11761 gagatcgtta ttgcctgcga caatctctgc gatcccaatg tgcatctcat ggcttccctt
    11821 acgcacatca ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc tggcaaaaat
    11881 ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat
    11941 gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca
    12001 cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagcgccga
    12061 aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccac
    12121 ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag
    12181 gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca
    12241 gcgggcaaaa cttttctccg tctctttttg caatgccctg acgtgctcgt tgcccgcagt
    12301 catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt
    12361 catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc ccgctttgtt
    12421 cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg
    12481 attgctctgc tcgacctgat tcagccgttg caaatcgtcg cgaaatgccc aaaaagacat
    12541 gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga
    12601 aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa
    12661 tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc gtggctgtgc
    12721 cggtgattgc tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc
    12781 tgccagacga aacgcggaaa ccgcttgcgt taaacgacta gcctgttctt ccagcgcagc
    12841 tgcagcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc
    12901 cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg ccgatgcaat
    12961 ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca ttgtttcccc
    13021 ggcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt caatgagagc
    13081 tttgatctct tttgccgcct gggcgctacg actggcaaga ttacgcactt cgcccgccac
    13141 cacggcaaaa ccacggccct gttcacccgc tcgcgcggct tcaaccgcgg cattcagcgc
    13201 gaggatatta gtctggaagg caataccgtc gattacgctg ataatgtcgg cgattttctt
    13261 cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg
    13321 ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt
    13381 ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc
    13441 ctgctgttcc gtacgggagg aaagatcggt gttacccgcc gcaatttcac gggtaccggc
    13501 atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat
    13561 atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt
    13621 cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca gcggtgtaag
    13681 caacatacgg cgaatgccat accacgccac cagcagaatc aataccacca ccagcgcgat
    13741 aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg
    13801 atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat
    13861 tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat aatcaatcag
    13921 ttcagttaac gctgtgtaat agtttttata tttttcatca atattacgac tggtagcgac
    13981 catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgcgg cctgggccaa
    14041 tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat tggaggaatc
    14101 catcatcatc cgcactgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca
    14161 ggttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa aactcttctg
    14221 gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca gtgcgaatac
    14281 ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc
    14341 tgaaaacaag ttgatctcgt tatcggcaag gaagggggaa actttattgc tgatgccacc
    14401 cgccgcgaaa ttgaaataaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa
    14461 atcaggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt
    14521 tcagcagttt ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag
    14581 tgagatattc tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg
    14641 tcaatgaaag cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat
    14701 tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc
    14761 gtaagtcaac aatcggcaca ataacaccgc gcagattcgt gacgcctttg ataaacgccg
    14821 gcgtgttcgc aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat
    14881 caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc
    14941 tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc
    15001 ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat
    15061 cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata
    15121 gttactttca aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa
    15181 ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtag cttcggtttt
    15241 cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac
    15301 ttccagcacc cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga
    15361 ttccataaca gcattcagcg gcaaaatgaa aacttcatcc gcaacgcgta cggacatgcc
    15421 gtcgaggatg gccagcgtca gcggcagtaa aatgcggatc gtcgtgccag taccctgctt
    15481 cgactggatt tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc
    15541 gacgccgcgc ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat
    15601 cagcatcgcg acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc
    15661 ttttgccaga attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat
    15721 gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc
    15781 gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag
    15841 cgggtcgata atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt
    15901 cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact
    15961 aaagacatat tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc
    16021 gttacgttgt aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc
    16081 gctggaacgc tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa
    16141 ttgatcaacc ttttctaccg ctacacggat gctggtggat tcactgctac gcgtcgtttt
    16201 ttcccgctcc acgcgaccgg ttggcgcttg ttcggctgcc agtttaagca ctggtggggt
    16261 ggatattttt ggcgagactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa
    16321 acagagtacc gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga
    16381 atccgcccct ttcaccacgt cagttaacgt tgtcagatgc cccagctctt cttccagcag
    16441 gtcgacttcg ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact
    16501 ctgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt
    16561 ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat cgaagctggc
    16621 ggcatccggc tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt
    16681 ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc
    16741 gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ccgaagccaa aagttcctgc
    16801 ccctcctttg atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc
    16861 ttccggctgc aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc
    16921 aaaaaatgtc tgataaaaat cgcttatatc catgctcacg ctgtcacctc ggttcggctg
    16981 atggcattgt gggaacactg acctgtggtg caacctcagg tttttccagg gcgcttactg
    17041 gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagaa
    17101 ccagcaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg
    17161 ttgccgccat gccgacgaca cgtaacactt tgccgctatc caacccgccg accatcagtt
    17221 cgcggcggga tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac
    17281 cgctggcgta gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt
    17341 tcagtacagg cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc
    17401 cggttttaaa catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga
    17461 ccagatcgat tttgagatgt ggacgtaacg cccgcagttt cggatcggac tctatcaact
    17521 ggtcgagatc accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcaactctt
    17581 cgatgttcgg ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg
    17641 ggattgggct ttcactatta gaaatgcgat tgccgcccgt aaccgctgtc gccagtggag
    17701 tccggaagta ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca
    17761 tcaccagaaa aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc
    17821 catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt
    17881 gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg
    17941 ctcttccagt tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc
    18001 aacggcgata ggcggtgcgt aaccgttcag attagaaagc agtgtgactt tgacgcactg
    18061 catcattttg ctggtttcgg cgcttttctg acgtaaaaca ctcgctaatg gggaaataaa
    18121 tccgtaagcc aataaaatgc cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc
    18181 cagctcggcg gcaggacgat cggctgaacc taacgcgtga acgaccccca ttacagccgc
    18241 gacaatacca aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc
    18301 tgcctcgctt tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt
    18361 catgtgaccg ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc
    18421 cgcgaggatg cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg
    18481 ttccagtgaa aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag
    18541 cagatccata tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt
    18601 cagcgtgcct ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc
    18661 ggcaataatc accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa
    18721 ataaccgccg aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc
    18781 atccttccac tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg
    18841 gcaaaaaaaa gcagcggtac gtcgttaccg ctgctggaat gttgcgccac atcgtatcag
    18901 ttaaacagcc tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg
    18961 ggaaagttta cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct
    19021 gccaacaggc tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag
    19081 ttgcagtaat ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag
    19141 cggtccttct tctgcttgcg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc
    19201 atcgacgcca ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga
    19261 agcatgaacg ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt
    19321 cggcggtggg cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg
    19381 tgtttcgctt tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg
    19441 aatatcccgc gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc
    19501 agcgcttctt caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta
    19561 tgaatttgct ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg
    19621 ctgtcaaaac ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga
    19681 agagtcagtg ccgctaatgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg
    19741 gacgctttgt cctgaacaat cagacgctgt gcaagtagta aatatgacaa gttgatgtca
    19801 taaatgtgtt tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat
    19861 ttttatgcgg tttcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta
    19921 gcattagaat agttgcgata agctgcaata agcagagcca cctttttggt ttaataagtc
    19981 cttacaaata gaagggggtc tttacactta tctaagattt ttcctaaatc gacgcaactg
    20041 tactcgtcac tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg
    20101 cagatcacac aaaacactca attacttaac ataaatgcat aagtcatact tttgttttgg
    20161 gtgtatttca atctgttaaa aagtttttcg ctacgctagc aagcaaaaat gaaacaggaa
    20221 taatcgaaat gggatgttgc gcacagttaa aataactcac cgtaaataat catctgctat
    20281 aaataatcac tttcatgcaa taccagataa gctattttta aacagacact taccgcacaa
    20341 caaactaatt aaatacaaag aaaggcaaca atcaacttaa tgttaataaa atgttttgca
    20401 tatattaacc acttgtttaa tgcggggaat cagtaaatta ttttcttata attacagttg
    20461 aaatcatatg atcaccgaga gtgattgatt aattgtcttt actaataatc agactaatat
    20521 ttacctgttt gactgagttg ggattgcgtc gtttctccat taggagtaaa gctttaatgt
    20581 cacctgaagt tcacagaata aggaacaggt tatgagctat agcaatattc ttgtcgctgt
    20641 tgcggttaca ccggaaagtc agcaactgct ggcaaaagcg gtatctatcg ccagaccagt
    20701 aaagggacac atcagtttaa ttactctcgc ttccgacccg gaaatgtaca atcaattagc
    20761 tgcgccgatg cttgaagatt tacgcagcgt gatgcaggaa gaaacgcaaa gctttcttga
    20821 taagttaatt caggatgcgg ggtatcccgt tgacaagacg tttattgcct acggtgagtt
    20881 aagcgaacat attctggaag tttgtcgcaa atatcatttc gatttggtga tttgcggtaa
    20941 tcacaatcac agtttctttt cgcgagcatc ctgctcggcg aaaagagtga ttgcctcaag
    21001 tgaagtcgat gtgctgttag ttccacttac ggggaattaa ccgctcctac gcaagcttag
    21061 gaaaggtagc aactttatcg cgctgctggc tttccgcgct tcgcggaact atctgcttta
    21121 ggtcgctgat gaagcactcc tgccagtggt taatatcgtt tttcacgata acgtccagca
    21181 tttctgcatg acgggaaata cgttccgcca gcgacatagt caatgcacga tccagcgcag
    21241 ctgcaacttc gtcacgatcg tagggattaa caattaacgc cgacgttaac tcgtttgccg
    21301 ctcccgcaaa ttgcgaaaga acaagaacgc ccggattggc tgggtcctga gcggcaacat
    21361 actcttttgc caccaggttc atcccgtcac gcagtggcgt cactaagccc acgtcagagt
    21421 agcggaatat tttcatcagt aatttacggt caaaatgctg attcaaataa taaagaggcg
    21481 tccagcctaa ttgcccgtat ttaccattaa ttcgtccagc ttcattttcg agctgatgac
    21541 gaatatcctg ataggcttgc acatcaccac gcgacgttgg tgcaatctgg gtataacgaa
    21601 ttttaccatg atgctgcgga tatttttcca gcaacgcttc ataggcgaga aaacgctctg
    21661 gcaaaccttt ggaataatcc agccgttcga cagaaaagat attttgtacg tttttcagtt
    21721 ccgctttaag ttgcgccagt tttggcggca gtggcccggc agcctgtttg gctatttctt
    21781 ttggctcaat gccaatcggg taaacttctg ttcgaaacgc tttgccccag gctgtatggc
    21841 tttttgcgct acgtgtcgtg acgcgggtca ggttagaaag gcaatccagg aacgccagac
    21901 gatcgttttc tgtctggaaa cccagcaaat cataatcaca aagctgttca agcaaggtgt
    21961 catatgtcgg cagcgcgttg aagatttccg gtgtcgggaa aggaatatgc agaaagaaac
    22021 caatgcgatt attcactccc cgtttgcgta attcatgcgc aaatggcaac aggtgataat
    22081 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
    22141 catttacgcg taaatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgaggcgat
    22201 aatgaaaagc aggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
    22261 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttcaccttt tttagcggct
    22321 gatcctcatt ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg
    22381 cccccagtat gccaacggca aggccaccgg cactggcggc gtgctcatct ggtggtgcaa
    22441 tccggttaga tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt
    22501 tgttgtaatg cggtggttat catttcaagc cagctccaga catccggtac gcccgccagt
    22561 cgccatgatg cctgagttgc gcctgtgcca atttttactg acattccgcc cagtcggtta
    22621 acgactgcga agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg
    22681 ataaagggag cttcctgcat aaaggcagct atcgcctcac ctttactggt acctctcggt
    22741 ttgatctcga caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata
    22801 cgttgcgcta atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca
    22861 aaagccatcc ctttcgtctc cagctccgcg ccgggatact gggcgatgac cgtatgcagt
    22921 tgcacgctaa tatcacgcgc aatcgcatcc ggcagatgaa cgatatgcgt tttaccattg
    22981 atgtcacggc gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt
    23041 gcgtccagct ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt
    23101 gccagtagct gaagtccttg cagaatattg tcaggtacga cgacctgatc gggatgcggt
    23161 ttgatttccg ccagcgttcc atcgagatca aaaaaccagg catatttcgc ggatagttca
    23221 ggggtttcgg ttaacggttc tgtcacccgg ttctcctcct tctcttttct tattgttttg
    23281 cgcagaacgc gctttacaga catgtaagta tagacagtgt gacgggggtc gccatttgca
    23341 acgaagaaca gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt tagattgttg
    23401 tggaaaaaag catcagacag tgcgtttcgc tttttgcttg taacggtcga agatcaccgc
    23461 tgccagcagg attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt
    23521 catggcgttt tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc
    23581 gatgccacct ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata
    23641 accaatcgac gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat
    23701 cgcagatacc aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc
    23761 cgccagacgc gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt
    23821 tttattcagc agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg
    23881 cagaccgaac cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc
    23941 tttaccgtcg gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt
    24001 gatcagagca tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaat
    24061 gccaagcagc aaccccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac
    24121 cgccgtggtg acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga
    24181 agcaaggcag aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa
    24241 ccctttcata ttaatgaagg tggcaaagtt cgggacaaaa atggcacagg cgataaaaag
    24301 caccgcaaac accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga
    24361 cttaggtgcg ccagaacccg atgtagaaac agaagacatc atactctcct tactcaggca
    24421 actgcctggc tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg
    24481 tgtaacaatt caccggcgat ttcaccttcc cgcatcacca caatccggtc ggcaacgccg
    24541 aggacttcag gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca
    24601 taaattacgt tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc
    24661 agcaaaatga ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt
    24721 ccgcctgaga gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga
    24781 atgtggtgat cggcattgtt ttcttcccaa ccgttgttga ttacacaacc gccgagcaca
    24841 tgtttacgtc tggcactgat gttgatattg tcgcgaacgg agtgcacagg aataatgcct
    24901 tccgctttgc gatcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta
    24961 cgaatatcga tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca
    25021 aacatgcctt tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt
    25081 tcaccactgc gaaccgccag acttattggc gtacgcacgc ctggtgcttt cacagcatca
    25141 agacgcaggc gctcctcgcc ataactacgc ggttgccagc cgtagatatc gccaatgtcg
    25201 cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt
    25261 ttgacataac gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc
    25321 atacggtgag aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg
    25381 aaaagattgt cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt
    25441 ttagcgttac gcgccagcgc cttggcgatt tcaaccatct gccactgacc aatggagaga
    25501 tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc
    25561 tcataattca gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag
    25621 atgttttccg cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca
    25681 cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca
    25741 gaacccgtgg ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg
    25801 ccattttcac ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc
    25861 agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac
    25921 tgttgcataa cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg
    25981 ggaatttttc cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag
    26041 ttgtcacgcg tgatcagcac cacgtcggta acttcggtga atttggtcgg ttcaacacct
    26101 tttgctaccc agttgtaaag catttcgctg gacttataac catgtacgtc cgggcttggc
    26161 aacagggaac cgtagaagcc ggttgcctgt gccttagaca gttcgctcac cgcatccaca
    26221 ccgttaatac cgataccgat gatatcggcc gctttaaagc cctgaccttc cgtcgcgcgt
    26281 acgccgccca gcacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga
    26341 tgttgaacca gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta
    26401 ggtacctgat aaatttgttt ttccgggaat cctgcagctt tcagcgcatc catagaaccc
    26461 gtagtacggc gacgagccgt atccagttcg ttggaggtaa tcgccatcac cgcgctttct
    26521 ttgacatccc agccacgttt ctgcatctct ttatacagtt cctggccctg acgttcgcca
    26581 attttggtcg ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta
    26641 acaaactggt catccacggc aatgactttc atatcgtagc cacgcgcctt cgccacgatg
    26701 gcagagccga gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggcggcc
    26761 aggctatcga tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca
    26821 aaccctaaat ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct
    26881 tccggttgct tcaccaggaa accgagcttc aggttctccg ccatagcgga ttgtgacata
    26941 acggctgcca gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc
    27001 tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca
    27061 aagcattacc ttttaactaa aagataagtg actgtgttga catagtttta gcgagaaatt
    27121 aattctccat aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt
    27181 agcgggaaaa gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga
    27241 ttaatccata agattagcct ggaaatcctt gttgtctttg gtacccatgc gggatgtctt
    27301 ctttttaacc agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa
    27361 ccacaactca aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc
    27421 tcattaatta acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta
    27481 taaaatcatc acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa
    27541 gcactatcat taaaattcat tgcagttaca ttgatctcat caatgaaatg taaaaatata
    27601 taaacttgat gatttaagca ttttcttata cccgttcaga cgttattctt atttcagatc
    27661 atcgtcagaa ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa
    27721 cagtaattat ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta
    27781 ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac
    27841 gcatccaatc tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc
    27901 gccactttcc ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac
    27961 tttatgaaga gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc cggtgaaaaa
    28021 ctgaactctc tggaagaact gttccaaaaa acgatggaag aatacgagca acgttctagt
    28081 acgttggcac agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc
    28141 ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat
    28201 gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg
    28261 aatgtcgtgt cgcatcagct gcattgatca tacatcggcg ctaatgcatt gcgccgatga
    28321 agtttttgag aaaccgctgc ctcatctttt tgaagcagcg gttttttaat gggattcgcc
    28381 ttgtggggta aacttgagtt caatcagcgc gatggctttt tggattgccc gcatggtgac
    28441 cgggtctgcg gcggcgggat gatgagtaaa gtcgatattc ttcagctggc tggacatttt
    28501 ttcgcgaact tcaacgggcg cgattacatc gagaacatcc agaatttgtt tgataaccaa
    28561 ctggcaagca accacatcag aaaccagttc ctgatcggca ttcagcggct gggacatcgt
    28621 aaactcctga tagcattttg aaagccgtta tagtagcggc ttcacatctt cagcgatagt
    28681 cacatccacc gtcatcagga cacaaaaaaa cctgccggag caggtttttt gttatcggaa
    28741 catattgcct ggcggtacgt ctttgaacgt cttgcaatag ttattgaaca tacttttcag
    28801 gattttgcgc agtttcatcg cggcactccg accatttgtt atacaggtgt tattgtcttt
    28861 gcgcttataa tatgacaacc atcacaaaaa tcaatcttta tgtgatgcaa atcacataaa
    28921 taccccttta atgttataaa aatgataacc aaaaaacagc ccccctattt ctgacaccta
    28981 cagatggcag gaaatagcgc ctgccaggcg tcttttccgg ccattgtcgc agcgctgtaa
    29041 cgcgtaaaat agtgctttct cttactcttc tggctggacc atgagacttc tgattctgac
    29101 tctttcatta ataacgctgg cggggtgtac ggttactcgc caggcccatg tgagcgaagt
    29161 tgacgcggca acaggtattg tacggttggt ttatgaccag gcttttttgc agcatgccca
    29221 taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca tgccagcagg aaggctatac
    29281 ccacgcgatc ccctttggtc agccagtagg caactgtagc ctttttgcgg gttccttatg
    29341 tttgaatacg gaattcactt tgtcatatca gtgccatcat tccgccttcc ctgtctttct
    29401 ctaaatattt taatcagcga ggggatcttc gctgattaaa gaaatagcgg taatgcgttt
    29461 tattcccatt cgtattttta ataattgaag tttatatttt accttttgca aataataaaa
    29521 taacaaatta tagtgacgcc acagcaacaa atataacctt tgtggagcac tatcatgctg
    29581 aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg
    29641 ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc
    29701 ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact
    29761 gataccggca atttaccgcg tattaatacc gttgaatctc cgtttgctga atattcctca
    29821 cttgatgaat tattccagga aacctataaa cacgaacaat taatcacgca gaaaattaac
    29881 gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt cctgcaatgg
    29941 tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc
    30001 ctggcaggca aaagcggcga aggtctgtat tttatcgaca aagaactctc taccctcgac
    30061 acacaaaact aatgctctcc ggcggcaggt tttctgccgc cgtttcaaat cagtgacggc
    30121 aaatcttact ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg
    30181 ccagaaacgc taccagctct cccgctgtca tcccttctgc cgagcaagtg tgaaaacgtg
    30241 cctgttcacc aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc
    30301 ctgattcaat catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc
    30361 aaaaaaaagc ctagcgtagc gattaccgct tatgaagact ttgcgccagc gcaggactga
    30421 atgcttttat tgtacattta aatttacacc atatgtaacg tcggtttgac gaagcagccg
    30481 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
    30541 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
    30601 tgctggcaat gccgctggct gcggccggtg ttggttttag cgttacttta atcttgttga
    30661 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
    30721 ttccggcaga taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
    30781 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg
    30841 gtgccggtga attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa
    30901 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc
    30961 tggtcgattt atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc
    31021 tggtattgct gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
    31081 ggctggcact gtctgcaatc ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc
    31141 cgagtattgt cagctatatg gatggcaaca ttcgtaagct acgctgggtg tttataaccg
    31201 gtagtgcgat ccccctggtg gcatatattt tctggcaggt ggcgacgctt ggcagcattg
    31261 attcaacaac ctttatggga ttgctggcta atcatgccgg attaaacggg ctgttacagg
    31321 cgttacgcga aatggtggcc tctccgcatg ttgagctggc agtgcattta tttgctgatt
    31381 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
    31441 tgtttcagcg ttcaaatacc gtgggtggaa gattgcaaac tggtgcaatt acctttctgc
    31501 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
    31561 gtgtggcgct ggcggtactg gcattgatta tcccttcgct gttgacctgg caaagcagaa
    31621 agcacaatcc tcaagcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
    31681 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
    31741 aagtggggtg attagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
    31801 caatttgact tcttaatgca ggcagcactg cttaaatttc ttaccactac cgcacgggca
    31861 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag cttgttcctg
    31921 cggatacgcc atccagtatg catgtaaatc aagcgccgcc agtcgaatgg catctacgct
    31981 ctcttcaaac gcttctggcg acatcttctc tacccgctcg aagttttcct cagtaccgtg
    32041 cagcgctatc gcctccagcg ctggttttaa cgaatcgggc aatgttgacc agtcagaaag
    32101 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
    32161 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
    32221 ggtatcggcc atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc
    32281 ccagcgcggc acatagtcag ccccacccca cacggcaacc agccactgtg ccggttcaat
    32341 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
    32401 ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
    32461 taacggtccc gttttcatac gcttttcctt gtggatctca actcgccagc acctatatta
    32521 catgccggtc cgtatcagag atactttttg agtggctttg ccggtgatta aaaatcaagg
    32581 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg gaggtggttt cacgacactg
    32641 cttattgctt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
    32701 cacagcg
//
LOCUS       DAZGCQ010000053        32239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000053 DAZGCQ010000000
VERSION     DAZGCQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.053"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(88..1848)
                     /gene="ycaO"
                     /locus_tag="ACHK07_002701"
     CDS             complement(88..1848)
                     /gene="ycaO"
                     /locus_tag="ACHK07_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO8103015.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSNNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1978..2670
                     /locus_tag="ACHK07_002702"
     CDS             1978..2670
                     /locus_tag="ACHK07_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO8103016.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2869..3957
                     /gene="serC"
                     /locus_tag="ACHK07_002703"
     CDS             2869..3957
                     /gene="serC"
                     /locus_tag="ACHK07_002703"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO8103017.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4028..5311
                     /gene="aroA"
                     /locus_tag="ACHK07_002704"
     CDS             4028..5311
                     /gene="aroA"
                     /locus_tag="ACHK07_002704"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO8103018.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKIIYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5480..6244
                     /gene="ycaL"
                     /locus_tag="ACHK07_002705"
     CDS             5480..6244
                     /gene="ycaL"
                     /locus_tag="ACHK07_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO8103019.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6417..7100
                     /gene="cmk"
                     /locus_tag="ACHK07_002706"
     CDS             6417..7100
                     /gene="cmk"
                     /locus_tag="ACHK07_002706"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO8103020.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7211..8884
                     /gene="rpsA"
                     /locus_tag="ACHK07_002707"
     CDS             7211..8884
                     /gene="rpsA"
                     /locus_tag="ACHK07_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO8103021.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9044..9328
                     /gene="ihfB"
                     /locus_tag="ACHK07_002708"
     CDS             9044..9328
                     /gene="ihfB"
                     /locus_tag="ACHK07_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO8103022.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9535..11799
                     /locus_tag="ACHK07_002709"
     CDS             9535..11799
                     /locus_tag="ACHK07_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO8103023.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQLTVNFSAQGWLISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11836..13584
                     /gene="msbA"
                     /locus_tag="ACHK07_002710"
     CDS             11836..13584
                     /gene="msbA"
                     /locus_tag="ACHK07_002710"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO8103024.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13581..14567
                     /gene="lpxK"
                     /locus_tag="ACHK07_002711"
     CDS             13581..14567
                     /gene="lpxK"
                     /locus_tag="ACHK07_002711"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO8103025.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14604..15836
                     /gene="ycaQ"
                     /locus_tag="ACHK07_002712"
     CDS             14604..15836
                     /gene="ycaQ"
                     /locus_tag="ACHK07_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO8103026.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15888..16070
                     /gene="ycaR"
                     /locus_tag="ACHK07_002713"
     CDS             15888..16070
                     /gene="ycaR"
                     /locus_tag="ACHK07_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO8103027.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16067..16813
                     /gene="kdsB"
                     /locus_tag="ACHK07_002714"
     CDS             16067..16813
                     /gene="kdsB"
                     /locus_tag="ACHK07_002714"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO8103028.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLKRVRAEMR"
     gene            16967..17860
                     /locus_tag="ACHK07_002715"
     CDS             16967..17860
                     /locus_tag="ACHK07_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO8103029.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17837..18616)
                     /gene="elyC"
                     /locus_tag="ACHK07_002716"
     CDS             complement(17837..18616)
                     /gene="elyC"
                     /locus_tag="ACHK07_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO8103030.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLSLLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRDNPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18752..19537
                     /gene="cmoM"
                     /locus_tag="ACHK07_002717"
     CDS             18752..19537
                     /gene="cmoM"
                     /locus_tag="ACHK07_002717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO8103031.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19534..20856
                     /gene="mukF"
                     /locus_tag="ACHK07_002718"
     CDS             19534..20856
                     /gene="mukF"
                     /locus_tag="ACHK07_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO8103032.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20837..21541
                     /gene="mukE"
                     /locus_tag="ACHK07_002719"
     CDS             20837..21541
                     /gene="mukE"
                     /locus_tag="ACHK07_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO8103033.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21541..26001
                     /gene="mukB"
                     /locus_tag="ACHK07_002720"
     CDS             21541..26001
                     /gene="mukB"
                     /locus_tag="ACHK07_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO8103034.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVEQ
                     RHLADQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26262..28109
                     /gene="ldtD"
                     /locus_tag="ACHK07_002721"
     CDS             26262..28109
                     /gene="ldtD"
                     /locus_tag="ACHK07_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8103035.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28290..28838
                     /locus_tag="ACHK07_002722"
     CDS             28290..28838
                     /locus_tag="ACHK07_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO8103036.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28865..29512
                     /gene="gloC"
                     /locus_tag="ACHK07_002723"
     CDS             28865..29512
                     /gene="gloC"
                     /locus_tag="ACHK07_002723"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO8103037.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLKEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29563..30753)
                     /gene="aspC"
                     /locus_tag="ACHK07_002724"
     CDS             complement(29563..30753)
                     /gene="aspC"
                     /locus_tag="ACHK07_002724"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO8103038.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30938..32011)
                     /gene="ompF"
                     /locus_tag="ACHK07_002725"
     CDS             complement(30938..32011)
                     /gene="ompF"
                     /locus_tag="ACHK07_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO8103039.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7846 a         7862 c         8677 g         7854 t
ORIGIN      
        1 aatatatgta aagcggggca ttaaaaaaac gccccgtaat ataactcaga cgaatcatta
       61 agcctacatt gcgtaggcta tttgatttta ttttgcccag aatgctgctt tggcgcgctg
      121 cagcttttcg taggccttca acagcgactg atgtgcagca aacgcgtgca gatcgctatc
      181 tactggttgc aagccataaa acgccgcttc gccgctcatt gccgcactag cggcttccac
      241 ggcatctgcg ccgtacatgc gaacaaacgc attcagatat tgcagcggct ggcgatcttc
      301 ttcctgcgcc agtaataaca acgtttgcag gcagcgataa tagttggcgc gttccgggct
      361 gaataccgat gagttaaact ccatcgtcca ttcggtccag accagagcct gttccagatc
      421 gccaccagcc agcgccagca tggcttttaa ttcaccgata cgcagggtgt accaaccgtt
      481 atccgacccg gtcgccagac ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc
      541 ttcatccagt tgctcgatga ggttcaggta atcttctttt tcccactcgc tgcctggtag
      601 cgagaggatg gtgtcgcgca gatgactacc catactgtta ttcgctaacc acaaatcttc
      661 agccggataa atatcggaca tgccaggcac gataatacgg caagcataaa cgcccagatg
      721 ctcgtaatcg gcaatataaa cttctttatc ttctttgttg aagatagcca tcagcgtggc
      781 aaactcttct tccgtggtgc cggagaaatt ccagtccaca aacggataat cggcatcctg
      841 cttgaacagg tcccaggaga ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt
      901 atgttcagcg acttcttcat catcgaaggt tggcggagta aacacatcca aatctttcag
      961 gccacgacct tgcagcagct cggtcacggt acgttccagt gctacgccaa aatcaggatg
     1021 cgcaccgaaa gaggcaaagc aggtgccgtt agcaggatta aacagtacca cgcaaatcac
     1081 cggatactgg ccgccaagcg aaccgtcata agcgaagatc gggaaacctt ccgcttccag
     1141 cgtttcgatc gcttcaacta ctgctggata acgtgccagc acgtctgccg ggatttccgg
     1201 caagctgatg ctttcagcaa taatgcggtt tttcacgtag cgttcgaaaa cttcggacaa
     1261 cccctgaacg cgtgcttcgt tacgggtatt gccagcagac ataccgttag aaacgtacaa
     1321 gttaccaatg atattcatcg gaatataaac ggtctgatta ttagactgac gtgtaaacgg
     1381 cagaccgcaa ataccacgat cttcgttacc ggattgtagg tcaatcagca tactgccggt
     1441 caattcattc tccggatcgt aaaatgcgcg cagacggtca tcgagcagcc cttctggcac
     1501 atcgtcattt tcggtcagtg ggaaccattt ttcgttggga taatgcacaa atggaccgtt
     1561 ggcgatggtt tcgcccagcc agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa
     1621 atattcaccg agtgcagaag ccagcgccgc tttcttggtt gcgcctttac cgttggtaaa
     1681 acacagtgcg cactctttgt cgcgaatatg tacagaccag acgttaggca cgggattcag
     1741 ccaggaggcc tcttcaatct gaaagccgag gtctgaaagt ttttgctgga agcgagcgat
     1801 ggaatcttcc agtgcggcat ctttgccggg gataaatgtt tgcgtcatga aaatcacttt
     1861 agtcgtacgg aaagcgcgca ataatacggg ttttatctca aaggcgctat caccgccgcc
     1921 atgccgatga actgttgact atgcttttag cggataaacc acgttaagag cataaaaatg
     1981 aaggcgttcg atctccaccg tatggcattt gataaagtgc cttttgattt ccttggcgaa
     2041 gttgcactac gtagtctgta tacctttgta ctggtttttt tgttcctcaa aatgaccgga
     2101 agacgcggag tgcggcagat gtcgctgttt gaagtgttaa tcattctgac gctggggtca
     2161 gcggcgggag atgtggcgtt ttatgatgat gtgccgatgg tcccggtact tatcgtcttt
     2221 atcactctgg cgttgttata ccgcctggta atgtggttga tggcacacag tgaaaaactg
     2281 gaagatcttc tggaaggtaa gccggttgtc attattgaag atggcgagct ggcctggtcg
     2341 aaactcaata actccaacat gacggaattt gagttcttta tggagctacg attgcgcggt
     2401 gtagagcagt tggggcaagt acgtctggcg attctcgaaa ccaacgggca aatcagtgtc
     2461 tatttctttg aagatgacaa ggtgaaaccg ggtttactta ttttacccag tgattgtacg
     2521 cagcgttaca aagtggtgcc ggagtcggcg gactatgcct gcatccgttg tagtgaaatc
     2581 attcatatga acgcggggga aaaacaatta tgtccgcgct gtgcaaatcc agaatggacg
     2641 aaggcaagtc gggctaaacg ggtgacctga cagcaaaaat atcggttttt tgctaataat
     2701 ccgagagatt ctgttgtgtg acgcgtgccg catttttgcc ctcaacggtt ttacccattg
     2761 cgatgcgtgt cactgaatga taaaaccgat agccacagga ataacatatt acccgcggtc
     2821 gcaatcgatt gactgggggt taatggcaac gcaacgtggt gaggggaaat ggctcaaatc
     2881 ttcaatttta gttctggtcc ggcaatgcta ccggcagagg tgcttaaaca ggctcaacag
     2941 gaactgcgtg actggaacgg tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa
     3001 gagttcattc aggttgcaga ggaagccgag aaggattttc gcgatcttct taatgtcccc
     3061 tccaactaca aggtattgtt ctgccatggc ggcggtcgcg gtcagtttgc tgctgttccg
     3121 ctgaatattc tcggtgataa aaccaccgca gattatgttg atgccggtta ctgggcggca
     3181 agtgccatta aagaagcgaa aaaatactgc acgcctaatg tctttgacgc caaagtgact
     3241 gttgatggtc tgcgcgcggt taagccaatg cgtgaatggc aactctctga taatgctgct
     3301 tatatgcatt actgcccgaa tgaaaccatc gacggtatcg ccatcgacga aacgccagac
     3361 ttcggcaaag atgtggtggt cgccgccgac ttctcttcaa ccattctttc ccgtccgatt
     3421 gacgttagcc gttatggcgt gatttacgct ggcgcgcaga aaaatatcgg cccggcaggc
     3481 ctgacaatcg tcatcgttcg tgaagatttg ctgggcaaag cgaatatcgc gtgtccgtcg
     3541 attctggatt attccatcct caacgataac ggctccatgt ttaacacgcc gccgacattt
     3601 gcctggtatc tgtctggtct ggtctttaaa tggctgaaag cgaacggcgg tgtagctgaa
     3661 atggataaaa tcaatcagca aaaagcagaa ctgctatatg gggtaattga taacagcgat
     3721 ttctaccgca atgacgtggc gaaagctaac cgttcgcgga tgaacgtgcc gttccagttg
     3781 gcggacagtg cgcttgacaa attgttcctt gaagagtctt ttgctgcagg ccttcatgca
     3841 ctgaaaggtc accgtgtggt cggcggaatg cgcgcgtcta tttataacgc catgccgctg
     3901 gaaggcgtta aagcgctgac agacttcatg gttgagttcg aacgccgtca cggctaatgc
     3961 cgaaattttg cttaatcccc acagccagcc tgtggggttt ttatttctgt tgtagagagt
     4021 tgagttcatg gaatccctga cgttacaacc catcgctcgt gtcgatggca ctattaatct
     4081 gcccggttcc aagagcgttt ctaaccgcgc tttattgctg gcggcattag cacacggcaa
     4141 aacagtatta accaatctgc tggatagcga tgacgtgcgc catatgctga atgcattaac
     4201 agcgttaggg gtaagctata cgctttcagc cgatcgtaca cgttgcgaaa ttatcggtaa
     4261 cggcggtcca ttacacgcag aaggtgccct ggagttgttc ctcggtaacg ccggaacggc
     4321 aatgcgtccg ctggcggcag ctctttgtct gggtagcaat gatattgtgc tgaccggtga
     4381 gccgcgtatg aaagagcgcc caattggtca tctggtggat gcgctgcgcc tgggcggggc
     4441 gaagatcatt tacctggaac aagaaaatta tccgccgttg cgtttacagg gcggctttac
     4501 tggcggcaac gttgacgttg atggctccgt ttccagccaa ttcctcaccg cactgttaat
     4561 gactgcgcct cttgcgccgg aagatacggt gattcgtatt aaaggcgatc tggtttctaa
     4621 accttatatc gacatcacac tcaatctgat gaagacgttt ggtgttgaaa ttgaaaatca
     4681 gcactatcaa caatttgtcg taaaaggcgg gcagtcttat cagtctccgg gtacttattt
     4741 ggtcgaaggc gatgcatctt cggcttctta ctttctggca gcagcagcaa tcaaaggcgg
     4801 cactgtaaaa gtgaccggta ttggacgtaa cagtatgcag ggtgatattc gctttgctga
     4861 tgtgctggaa aaaatgggcg cgaccatttg ctggggcgat gattatattt cctgcacgcg
     4921 tggtgaactg aacgctattg atatggatat gaaccatatt cctgatgcgg cgatgaccat
     4981 tgccacggcg gcgttatttg caaaaggcac caccacgctg cgcaatatct ataactggcg
     5041 tgttaaagaa accgatcgcc tgtttgcgat ggcaacagaa ctgcgtaaag tcggtgcgga
     5101 agtagaagag gggcacgatt acattcgtat cactccaccg gaaaaactga actttgccga
     5161 gatcgcgaca tacaatgatc accggatggc gatgtgtttc tcgctggtgg cgttgtcaga
     5221 tacaccagtg acgattcttg atcccaaatg tacggccaaa acatttccgg attatttcga
     5281 gcagctggcg cggattagcc aggcagcctg aatgaataac gtccaataaa tagccaaatc
     5341 tttctttatc aaaacgccgg cacattgccg gcgttttttt tcggaccttg tgagtcattt
     5401 tgattaatgg tagcgtcgct tgtcaatgta agttgttgat acataatatt tatatatgat
     5461 taatcaacgg atgattcaca tgaagaatac taaattactg ctggcgattg cgacctctgc
     5521 agcattactg acagggtgtc aaaataccca cggtattgat accaatatgg ctatcagctc
     5581 cggtttaaat gcctataaag cagcaacgtt aagcgatgcc gatgcaaaag cgattgccaa
     5641 tcagggctgt gccgaaatgg acagtggcaa tcaagtcgca agtaaatcca gcaagtacgg
     5701 taaacgtctg gcaaaaatcg ccaaagcatt gggtaacaat attaatggca cgccggtcaa
     5761 ctataaggtt tatatgacca gcgacgtcaa cgcatgggcg atggcgaacg gctgtgttcg
     5821 tgtctacagt ggcctgatgg acatgatgaa tgacaacgaa attgaaggtg ttctgggcca
     5881 tgaactgggc cacgtcgcgt tgggtcactc gctggctgaa atgaaagctt cttatgcgat
     5941 cgttgccgca cgcgatgcca tttcagctac cagcggtgtg gcttcccagc tttcccgctc
     6001 acaattaggt gatatcgcag aaggcgctat caatgcgaaa tactcccgtg ataaagagtc
     6061 cgaagcagat gatttctcct ttgatctgtt gaagaaacgt ggcatcagca cccaggggct
     6121 ggttggcagc tttgaaaaac tggctagcct ggatggcggt cgcacccagt ccatgtttga
     6181 ctctcaccca ccatcaacag agcgtgcgca acacatccgt gatcgtatcg cctctggtaa
     6241 gtaaatcatt gtcatctttc gggctggtct tctgccagcc cgctataatt gcgcaataaa
     6301 tccccatctg aatacagaca aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc
     6361 ggcgcgtata atgcgcgcgg ttatgttaac ggtacgcctg ttttaaggag ataaagatga
     6421 cggcaattgc cccggttatt accattgatg gcccaagcgg tgcagggaaa ggcaccttgt
     6481 gtaaggctat ggcggaagcg ttgcaatggc atctgctgga ctcgggtgca atttatcgcg
     6541 tactggcact agcggcatta catcaccatg ttgatgttgc ttcggaagat gcgctggtgc
     6601 cgctggcatc ccatctggat gtacgttttg tgtcgaccaa tggcaatctg gaagtgatcc
     6661 tcgaagggga agatgtcagc ggcgaaattc gtactcagga agtggcgaat gctgcttcac
     6721 aagtcgcggc attcccacgc gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg
     6781 aattaccagg cctgattgcc gatggccgcg acatgggaac ggtggtattc cctgatgcgc
     6841 cagtgaaaat tttccttgac gcctcctcgg aagaacgtgc gcatcgccgc atgctacagt
     6901 tgcaggagaa gggctttagt gttaactttg agcgcctttt ggccgagatc aaagaacgcg
     6961 acgaccgcga tcgtaaccga gcggtagcgc cactggttcc ggcagccgat gctttagtgt
     7021 tggattccac caccttaagc attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc
     7081 agaaattggc tctcgcataa gcgaccgaat ttgcagtacc cccgttgcaa tggaatgaca
     7141 gcgggtatgt taaacaaccc catccggcat ggagccaggt ggacgttaaa tataaacctg
     7201 aagattaaac atgactgaat cttttgctca actctttgaa gagtccttaa aagaaatcga
     7261 aacccgcccg ggttctatcg ttcgtggcgt tgttgttgct atcgacaaag atgtagtact
     7321 ggttgacgct ggtctgaaat ctgagtccgc catcccggct gagcagttca aaaacgccca
     7381 gggcgagctg gaaatccagg taggtgacga agttgacgtt gctctggacg cagtagaaga
     7441 cggcttcggt gaaactctgc tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac
     7501 gctggaaaaa gcttacgaag atgctgaaac tgttaccggt gttatcaacg gcaaagttaa
     7561 gggcggcttc actgttgagc tgaacggtat tcgtgcgttc ctgccaggtt ccctggtaga
     7621 cgttcgtccg gtgcgtgaca ctctgcacct ggaaggcaaa gagcttgaat ttaaagtaat
     7681 caagctggat cagaagcgca acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga
     7741 aaacagcgca gagcgcgatc agctgctgga aaacctgcag gaaggcatgg aagttaaagg
     7801 tatcgttaag aacctcactg actacggtgc attcgttgat ctgggcggcg ttgacggcct
     7861 gctgcacatc actgacatgg cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt
     7921 gggcgacgaa atcactgtta aagtgctgaa gttcgaccgc gaacgtaccc gtgtatccct
     7981 gggcctgaaa cagctgggcg aagatccgtg ggtagctatc gctaaacgtt atccggaagg
     8041 taccaaactg actggtcgcg tgaccaacct gaccgactac ggctgcttcg ttgaaatcga
     8101 agaaggcgtt gaaggcctgg tacacgtttc cgaaatggat tggaccaaca aaaacatcca
     8161 cccgtccaaa gttgttaacg ttggcgatgt agtggaagtt atggttctgg atatcgacga
     8221 agaacgtcgt cgtatctccc tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt
     8281 cgcggaaacc cacaataagg gcgaccgtgt tgaaggtaaa atcaagtcta tcactgactt
     8341 cggtatcttc atcggcctgg acggcggcat cgacggcctg gttcacctgt ctgacatctc
     8401 ctggaacgtt gcaggcgaag aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc
     8461 agttgttctg caggttgacg cagaacgtga acgtatctcc ctgggcgtta aacagctcgc
     8521 agaagatccg ttcaacaact gggttgctct gaacaagaaa ggcgctatcg taaccggtaa
     8581 agtaactgca gttgacgcta aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta
     8641 cctgcgtgct tctgaagcat cccgtgaccg cgttgaagac gctaccctgg ttctgagcgt
     8701 tggcgacgaa gttgaagcta aattcaccgg cgttgatcgt aaaaaccgtg caatcagcct
     8761 gtctgttcgt gcgaaagacg aagctgacga gaaagatgca atcgcaactg ttaacaaaca
     8821 ggaagatgca aacttctcca acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga
     8881 gtaattctct gactcttcgg gatttttatt ccgaagattg ttgagtttac ttgacagatt
     8941 gcaggtttcg tcctgtaatc aagcactaag ggcggctacg gccgccctta atcaatgcag
     9001 caacagcagc cgcttaattt gcctttaagg aaccggagga atcatgacca agtcagaatt
     9061 gatagaaaga cttgccaccc agcaatcgca cattcccgcc aagacggttg aagatgcagt
     9121 aaaagagatg ctggagcata tggcctcgac tcttgcgcag ggcgagcgta ttgaaatccg
     9181 cggtttcggc agtttctctt tgcactaccg cgcaccacgt accggacgta atccgaagac
     9241 tggcgataaa gtagaactgg aaggaaaata cgttcctcac tttaaaccag gtaaagaact
     9301 gcgcgatcgc gccaatattt acggttaagt ttttactcaa acttgaacgc gagaaaagca
     9361 cctgtcgggt gcttttttca tttctctaat caggaactgg aagctgcctc gcagagtttt
     9421 gagcagtttt caccctttcg ttaaattctt ctgaatgtgc ctcggggaac gcaaaattcc
     9481 caccagacaa ccgctcaaca aagttgcaca ctttccataa acagggaggg gtgcatgaaa
     9541 ataacgacag tcggcgtatg cataatttgc ggaatttttc cgttgctgat tttgccccaa
     9601 ttgcctggga cattaaccct tgcgtttctg actctcttcg cctgcgtact ggcatttatc
     9661 cctgttaaaa ccgtccgtta tatcgcgctg acgttgctgt ttttcgtttg gggcatatta
     9721 tcagcaaagc aaattttgtt ggcaggagaa accttaactg gcgcgacgca ggatgcaatt
     9781 gttgagatca ctgctactga cggcatgacc actcattacg gtcaaattac ttatctacaa
     9841 ggtcgacgta tattccctgc gccaggcctt gtgctgtatg gcgaatatct tccgcaagcg
     9901 gtttgtgccg gacaactgtg gtcaatgaaa ctcaaagttc gtgccgttca tggtcaactt
     9961 aatgatggcg gctttgatag ccagcgttat gccattgccc agcatcagcc gctcaccggc
    10021 cgctttctgc aggcaagtgt tattgaaccg aattgtagcc tgcgtgcaca gtatctggcg
    10081 tcactacaaa caacgctgca accctatccg tggaatgcgg ttattcttgg tttaggtatg
    10141 ggggaacggt tatccgtccc taaagaaatc aaaaatatca tgcgtgatac tggaacggcg
    10201 catttaatgg cgatatcggg attgcacatc gcttttgcgg cgttgctggc tgccggactc
    10261 attcgcagtg gacaaatttt tctgcctggg cgctggatcc actggcaaat gccattaatt
    10321 ggcggaatct gctgtgctgc tttttatgcc tggctgacgg gaatgcaacc tcctgcattg
    10381 cgtaccgttg tggcgcttgc tacgtgggga atgcttaagt taagtgggcg acagtggagt
    10441 ggctgggatg tatggatatg ttgtctggcg gcaattttgc tgatggatcc tgttgccatt
    10501 ctctcgcaaa gtttatggct ctctgccgct gcggtcgcgg cattgatatt ttggtatcag
    10561 tggtttccct gtcctgagtg gcaactgccg ccggtattgc gtgcagttgt ttccctcatt
    10621 catttgcaac tgggaatcac acttctgctt atgcccgtgc aaatcgtcat atttcatggc
    10681 attagtctga cctcgtttat tgcaaatcta ttagcaattc ccttggtgac atttatcacg
    10741 gttccgctga tcctcgccgc gatggttgtg catttaagcg ggccgttaat cctggagcaa
    10801 gggttatggt ttcttgccga ccggtctttg gctttacttt tctgggggtt aaagagtttg
    10861 ccagaagggt ggatcaacat tgctgaacgt tggcaatggc tatcattttc cccatggttc
    10921 ttactggtgg tatggcgatt aaacgcctgg cgaacgttgc cagcaatgtg tgtggctgta
    10981 ggcttgctga tgtgctggcc gctgtggcaa aaacctcgac ctgacgagtg gcaagtgtac
    11041 atgcttgatg tcgggcaagg gctggcaatg gtgatagcca gaaacggcaa agcgattctc
    11101 tatgacacgg gactggcctg gcccgaaggg gatagtgggc aacaactgat tatcccctgg
    11161 ctccactggc ataatcttga accggaaggc gttattctga gtcatgaaca tctggatcac
    11221 cggggagggc tggactcaat attgcacaca tggccgatgt tatggatcag aagtccgtta
    11281 aactgggaac atcatcagcc ctgtgtgcgt ggcgaagcgt ggcaatggca aggattgcgt
    11341 ttcagcgtgc actggccttt acaagctagc aacgataaag gaaataacca ttcctgtgtg
    11401 gttaaggttg atgacgggac gaatagcatt cttctaaccg gtgatattga agtccccgct
    11461 gaacaaaaga tgctaagccg ttactggcag caagtgcagg caacattgct tcaggtacct
    11521 caccatggca gtaatacctc atcatcgttg ccattaattc agcgagtgaa tggaaaagtg
    11581 gcactcgcat cggcatcgcg ctataacgca tggcgactgc cctctaacaa agttaagcat
    11641 cgctatcaac aacagggtta tacgtggctt gatactcctc atcaggggca actaacggtc
    11701 aatttttcag cacaaggctg gctgattagc agcctcaggg agcaaatttt acctcgttgg
    11761 tatcatcagt ggtttggcgt gccagtggat aacgggtaga atatgcggct atttcaacaa
    11821 atgctggttt tttgaatgca taacgacaaa gatctctcta cgtggcagac attccgccga
    11881 ctgtggccaa ccattgcgcc ttttaaagcg ggtctgatcg tggcgggcgt agcgttaatc
    11941 ctcaacgcag ccagcgatac cttcatgtta tcgctcctta agccacttct tgatgatggc
    12001 tttggtaaaa cagatcgctc cgtgctggtg tggatgccgc tggtggtgat cgggctgatg
    12061 attttgcgtg gtatcaccag ctatgtctcc agctactgta tctcctgggt atcaggaaag
    12121 gtggtaatga ccatgcgtcg ccgcctgttt ggtcacatga tgggaatgcc ggtttcattc
    12181 tttgacaaac agtcaacggg tacgctgttg tcacgtatca cctacgattc cgaacaggtt
    12241 gcttcttcct cttccggcgc actgattact gttgtgcgtg aaggtgcgtc gatcatcggc
    12301 ctgttcatca tgatgttcta ttacagttgg caattgtcga tcattttgat tgtgctggca
    12361 ccgattgttt cgattgcgat tcgcgtagta tcgaagcgtt ttcgcaacat cagtaaaaac
    12421 atgcagaaca ccatggggca ggtgaccacc agcgctgaac aaatgctgaa aggccataaa
    12481 gaagtattga ttttcggtgg tcaggaagtg gaaacgaaac gcttcgataa agtcagcaac
    12541 cgaatgcgtc ttcaggggat gaaaatggtt tcagcctctt ccatctctga tccgatcatt
    12601 cagctgatcg cctctttggc gctggcgttt gttctgtatg cggcgagctt cccaagtgtc
    12661 atggatagcc tgactgccgg tacgattacc gttgttttct cttcaatgat tgcactgatg
    12721 cgtccgctga aatcgctgac taacgttaac gcccagttcc agcgcggtat ggcggcttgt
    12781 cagacgctgt ttaccattct ggacagtgag caggagaaag acgaaggtaa gcgcgtgatc
    12841 gagcgtgcga ctggcgacgt ggaattccgc aacgtcacct ttacttatcc gggacgtgac
    12901 gtacccgcat tgcgtaacat caacctgaaa attccggcag ggaagacggt tgctctggtt
    12961 ggacgctctg gttcaggtaa atcaaccatc gccagcctga tcacgcgttt ttacgatatt
    13021 gatgaaggcg aaatcctgat ggatggtcac gatctgcgcg agtataccct ggcgtcgtta
    13081 cgtaaccagg ttgctctggt gtcgcagaat gtccatctgt ttaacgatac ggttgctaac
    13141 aacattgctt acgcacggac tgaacagtac agccgtgagc aaattgaaga agcggcgcgt
    13201 atggcctacg ccatggactt catcaataag atggataacg gtctcgatac agtgattggt
    13261 gaaaacggcg tgctgctctc tggcggtcag cgtcagcgta ttgctatcgc tcgagccttg
    13321 ctgcgtgata gcccgattct gattctggat gaagctacct cggctctgga taccgaatcc
    13381 gaacgtgcga ttcaggcggc actggatgag ttgcagaaaa accgtacctc tctggtgatt
    13441 gcccaccgct tgtctaccat tgaaaaggca gacgaaatcg tggtcgttga ggatggtgtc
    13501 attgtggaac gcggtacgca taacgatttg cttgagcacc gcggcgttta cgcgcaactt
    13561 cacaaaatgc agtttggcca atgatcgaaa aaatctggtc tggtgaatcc cctttgtggc
    13621 ggctattgct gccactctcc tggttgtatg gcctggtgag tggcgcgatc cgtctttgct
    13681 ataaactaaa actgaagcgc gcctggcgtg cccccgtacc ggttgtcgtg gttggtaatc
    13741 tcaccgcagg cggcaacgga aaaaccccgg tcgttgtctg gctggtggaa cagttgcaac
    13801 agcgcggtat tcgcgtgggg gtcgtgtccc ggggatatgg tggtaaggct gaatcttatc
    13861 cgctgttatt gtcggcagat accaccacag cacaggcggg tgatgaacct gtgttgattt
    13921 atcaacgcac tggcgcgccc gttgcggttt ctcccgtgcg ttctgatgcg gtaaaagcca
    13981 ttctggcgca acaccctgat gtgcagatca tcgtaaccga cgacggttta cagcattacc
    14041 gtctggcgcg tgatgtggaa attgtcgttg ttgatggtgt gcgtcgcttt ggcaatggct
    14101 ggtggttgcc ggcggggcca atgcgtgagc gagcggggcg cttaaagtca gttgatgcgg
    14161 taatcgtcaa cggcggtgtc ccccgcagcg gtgaaatccc catgcatctg ctgccgggtc
    14221 aggcggtgaa tttacgtacc ggtacgcgtt gtgacgttgc tcagcttgaa catgtggtgg
    14281 cgatggcagg gattgggcat ccgccgcgct tttttgccac gctgaagatg tgcggcgtac
    14341 aaccggaaaa atgtgtaccg ctggccgatc atcagtcttt gaaccatgcg gatgtcagcg
    14401 cgttggtaag caccgggcaa acgctggtaa tgactgaaaa agatgcggtg aaatgccggg
    14461 cctttgcaga agaaaattgg tggtatttgc ccgttgacgc acagctttca ggtgatgaac
    14521 cagcgaaact gcttgcgcaa ctaacctcgc tggcttctgg caactagtta cgccgcggca
    14581 gcgttcgatt gatggagtca tgaatgtcgc tgccgcacct ctcccttgct gatgcgcgta
    14641 atcttcacct tgccgcgcaa ggcctgttaa acaaaccccg ccgtcgagcg tcgttggagg
    14701 atattccggc aacgatctcc cgcatgtcct tgctgcaaat cgataccatc aatattgttg
    14761 cccgtagccc atatctggtg cttttcagtc gtctgggaaa ttatcctgcc cagtggctgg
    14821 atgagtctct ggcgcgtggc gaattaatgg aatactgggc gcatgaagcc tgctttatgc
    14881 cacgcagcga ctttcgtctt attcgccacc gcatgctggc acctgaaaaa atgggctgga
    14941 aatacaaaga cgcctggatg caggaacatg aggcggaaat tgcacagtta attcagcata
    15001 ttcatgataa ggggccggta cgttcagccg attttgagca tcctcgtaaa ggtgcaagcg
    15061 gctggtggga atggaagccg cataaacggc atctggaagg tttatttact gccggaaagg
    15121 tgatggtgat tgaacggcgc aacttccagc gcgtttatga tttaacccac cgtgtcatgc
    15181 ctgactggga tgatgagcgc gatctcgttt cgcaaacaga agcagaaatc atcatgctgg
    15241 ataacagtgc gcgtagcctg ggaatattcc gcgaacagtg gctggcagat tactatcggc
    15301 tgaaacgtcc ggcactggca gcgtggcgcg aagcgagggc tgaacagcgg caaatcattg
    15361 ctgtgcatgt tgaaaaattg ggcaatcttt ggctgcatgc tgatttgctg ccgctactcg
    15421 agcgagcgct ggccggaaag ctcactgcaa cgcacagcgc ggtactttcg ccttttgatc
    15481 ctgttgtctg ggatcgcaaa cgcgcagagc agctttttga ttttagctac cggctggagt
    15541 gctatacccc agcgccgaaa cgccagtatg gctattttgt tctgccgtta ttacatcgtg
    15601 ggcaattagt tgggcgaatg gatgccaaaa tgcatcgcca gacaggcatc cttgaagtta
    15661 tctctctgtg gttacaggaa ggtattaaac caacgacaac gctgcaaaaa gggttacgtc
    15721 aggcgattac tgatttcgct aactggcagc aggcaacgcg ggtgacatta ggacactgcc
    15781 cgcaaggtct ctttacggat tgccgcaccg gctgggaaat agaccccgtc gcataaatga
    15841 ttatgataag atttatgcat tcataacccg atccatctgg aggaactatg gatcatcgtc
    15901 tgcttgaaat cattgcctgc ccggtttgca acggaaaact ttggtataac caggaaaaac
    15961 aagagcttat ttgcaaactg gacaacctgg ctttcccgct gcgcgatggc attccagtgt
    16021 tgctggaaac ggaagcccgc gtgctgactg ctgatgagag taaatcatga gttttgtggt
    16081 cattattccc gcgcgctacg cgtcaacgcg tctgcccggt aaaccattgg ttgatattaa
    16141 tggcaaaccc atgattgttc atgttcttga acgtgctcgc gaatcaggtg ccgagcgcat
    16201 catcgtggca accgatcatg aggatgtcgc ccgcgccgtt gaagccgctg gcggtgaagt
    16261 atgtatgacg cgcgccgatc accagtcagg gacagaacgc ttggcggaag ttgtcgaaaa
    16321 atgcgcgttc agcgacgaca cggtgatcgt taatgtgcag ggtgatgaac cgatgatccc
    16381 tgcgacaatc attcgtcagg ttgctgataa cctcgctcag cgtcaggtgg gtatggcgac
    16441 tctggcggtg ccaatccaca atgcggaaga agcgtttaac ccgaatgcgg tgaaagtggt
    16501 tctcgacgct gaagggtatg cactgtactt ctcacgcgct accattccgt gggatcgtga
    16561 tcgttttgca aaaggccttg aaaccgttgg cgataacttc ctgcgtcatc ttggtattta
    16621 tggctaccgt gcaggcttta tccgtcgtta cgtcacctgg cagccaagcc cgttagaaca
    16681 catcgaaatg ttagagcagc ttcgtgttct gtggtacggc gaaaaaatcc acgttgctgt
    16741 cgcgcatgaa gttcctggca caggtgtgga tacccctgaa gatctcaagc gcgttcgcgc
    16801 tgaaatgcgc taatttcact tcacgacact tcagccaatt ttgggaggag tgtcgtaccg
    16861 ttacgatttt cctcaatttt tcttttcaac aattgatctc attcaggtga catcttttat
    16921 attggcgctc attatgaaag cagtagcttt tatgagggta atctgaatgg aacagctgcg
    16981 tgccgaatta agccatttac tgggcgaaaa actcagtcgt attgagtgcg tcaatgaaaa
    17041 agcggatacg gcgttgtggg ctttgtatga cagccaggga aacccaatgc cgttaatggc
    17101 aagaagcttt agtacgcccg gaaaagcccg acaactggca tggaaaacca ccatgctggc
    17161 aagaagtggg actgtccgta tgccgactat ttatggtgtg atgacgcatg aagaacaccc
    17221 cggccctgat gtcctgctac tggagcggat gcgtggtgtt tcggtggagg caccagcccg
    17281 aacaccagaa cgttgggaac agctcaaaga ccaaatcgtt gaagccttac tggcctggca
    17341 ccgtcaggac agtcgcggtt gcgtcggcgc ggtcgacaat actcaggaaa atttctggcc
    17401 ctcatggtac cggcaacatg ttgaagtgct atggaccacg ctcaatcagt tcaataacac
    17461 cggtctgacg atgcaggata agcggatcct gtttcgcact cgcgaatgtc tcccggcatt
    17521 atttgaaggc tttaacgaca attgtgtgtt gattcacggt aacttctgtt tacgcagcat
    17581 gctgaaagat tcgcgcagcg atcagttact ggcgatggtc gggccgggac taatgctttg
    17641 ggcaccgcga gaatacgaac tgttccgact aatggataat tctctggcgg aagatttgct
    17701 ctggagttac ctgcaacgcg cgccagtggc ggagtcgttc atctggcggc gttggttgta
    17761 tgtgttatgg gatgaagttg cgcaactggt taataccgga cgatttagtc ggcgcaactt
    17821 cgatctggca tcaaaatcac tcttgccgtg gctcgcctga cggacctttc agccactgcc
    17881 agatgcggcc aagcgtttca tagccaacac gatcgctatg catcagccag accggagaag
    17941 ggattgcccg ttcccacgga ttaagcggcg agtcgatggc caactgattc gccggggctg
    18001 gtagcggatt taacccttcc tgctgaaaaa agatcatcgc ccgcggcaga tgtgatgcgg
    18061 aggtcaccag caggaaaggg gcgtcaccaa tcgcctgttt cactgccgca gcttcttctt
    18121 cggtatcttt tggcaaatcc agggtgataa tttgctcgcg cggtacgccc agcgattgcg
    18181 caactctggc acctacttcc gctgtactca ccgtattggt ttttgctacg cctcccgtga
    18241 agatcagttt tgaacccgga ttatcgcgcc ataaacggat accctcattc aggcgtggca
    18301 gactgttatt gattaaatta gagctcggtg cccactgcgg gttccaggta taaccacctc
    18361 cgagcaccac gatatagtcc actttttgcg aattattcca tgtcggatag gtgctttcga
    18421 tgggacgcag caggcggtcg gctaccggtt gcaaactcaa taacaagagt gacagccacc
    18481 caatgctaat aaaaatcttc ccggtttttt gaaaacggct aaaccacagc agcgccaggc
    18541 cagcgccaat aattaacaac atcagcggaa gtggcagcaa catattgcca atcacttttt
    18601 tcagtgtaaa aagcatcctt tctggttcct tttttaacca tatagcaagg gatcgccagc
    18661 gatattacac cagacgggtt cattctcgcc gtggctgtga caaaatagcg tctttatcag
    18721 aaaacgtctt agccaaccgg tggagaaagc aatgcaggat cgcaattttg atgatattgc
    18781 ggaaaagttt tcccgtaaca tttacggcac caccaaaggg cagcttcgac aggctattct
    18841 gtggcaggat ctcgatcgcg tgctggcgga aatgggtccg caaaaactgc gtgtgctgga
    18901 tgcgggcggt ggagaagggc agaccgcaat caaaatggcc gagcgcgggc atcaggtcat
    18961 tttatgcgat ctttctgcgc agatgatcga ccgcgcaaaa caggcggcag aagcaaaagg
    19021 tgtgagcgac aacatgcaat ttatacattg cgccgctcag gatgttgctt cgcatttgga
    19081 aacgcccgtt gatctgatat tgttccatgc ggtgctcgag tgggtggctg atccccgcag
    19141 cgtattgcag accctgtggt cagtgttgcg tccaggcggc gtgttgtcgt taatgttcta
    19201 caatgcgcat ggtttgttga tgcataacat ggtcgccggg aattttgatt acgtgcaggc
    19261 gggaatgccg aaaaagaaaa aacggacgct ttcgccagat tatccacgcg acccggcgca
    19321 ggtttatctg tggctggaag aagctggttg gcaaattatg ggtaagacag gcgttcgcgt
    19381 gtttcatgat tatctgcgcg agaagcacca gcagcgcgac tgctatgaag cattacttga
    19441 attagaaacg cgttattgcc gtcaggaacc gtatattacc ctggggcgtt atattcatgt
    19501 caccgcgcgc aaaccgcaga gcaaggataa agtatgagtg aattttccca gacagtcccc
    19561 gaactggttg cctgggccag aaaaaatgac ttctccatct cgctgccggt agaccgactc
    19621 tcttttctgc tggcggttgc cacgctgaac ggcgagcgtc tggatggtga gatgagtgaa
    19681 ggcgagctgg tggatgcatt ccgccatgtg agtgatgcgt ttgagcaaac cagcgaaacc
    19741 atcggcgtgc gcgccaataa cgcgatcaac gacatggtgc gtcaacgtct gctgaaccgc
    19801 tttaccagcg agcaggcaga agggaacgca atttaccgtc tgacgccgct cggcatcggc
    19861 attactgact attacatccg tcagcgcgag ttttctacgc tgcgcctttc tatgcagttg
    19921 tcgattgtgg cgggtgagct caaacgcgca gcagatgccg ccgaagaggg cggtgatgaa
    19981 tttcactggc accgtaatgt ctatgcgcca ctgaaatatt cggtagcaga aattttcgac
    20041 agtatcgacc tgacgcaacg tctgatggac gaacagcagc agcaggtgaa ggacgatatc
    20101 gcccagttgc tgaacaaaga ctggcgggcg gcgatttcca gctgtgaatt gttgctttcg
    20161 gaaacttccg gaacgctgcg tgaattgcag gatacactgg aagcggcagg cgacaaattg
    20221 caggctaatc tgttgcgcat tcaggatgcg acgatgactc atgacgatct gcattttgtc
    20281 gatcgtctgg tgttcgatct gcagagcaaa ctcgaccgta ttatcagttg gggccagcaa
    20341 tccatcgact tgtggattgg ctacgaccgc cacgtacaca aatttattcg taccgcgatc
    20401 gatatggata aaaaccgcgt ctttgctcag cggttacgtc agtcggtaca aacctatttt
    20461 gatgagccgt gggcgctaac ttatgccaat gccgatcgtc tgctggatat gcgtgacgaa
    20521 gagatggcac tgcgcgatga agaagtgact ggggaacttc ctgaggatct ggaatacgaa
    20581 gagtttaacg agatccgcga acagctggcg gcgatcatcg aagaacaact tgctgtgtac
    20641 aaaaccagac aagtgccgct ggatcttggt ctggtggtac gcgaatatct gtcacagtat
    20701 ccgcgtgcac gtcactttga cgttgcgcgc attgttattg atcaggcggt acgtcttggc
    20761 gtagcgcaag cagatttcac cggactgcca gcgaaatggc agccgattaa tgattacgga
    20821 gccaaggtac aggcgcatgt catcgacaaa tattgaacaa gtgatgccgg ttaagctggc
    20881 gcaggcgctg gcgaatccgt tatttccggc gctggacagc gccttacgtt caggccgcca
    20941 tattggcctc gacgaactgg ataatcatgc attcctgatg gattttcagg aatatctgga
    21001 agagttttac gcgcgttata acgttgagct tattcgtgca ccggaagggt tcttctattt
    21061 acgtccacgt tccaccacgc tgatccctcg ttccgtcttg tcggaactgg atatgatggt
    21121 cgggaaaatc ctctgctatc tctatctcag cccggaacgg ctggcgaatg aggggatttt
    21181 tactcaacag gaactgtacg acgaactgct caccctggcc gatgaagcga aactgctgaa
    21241 actggtgaac aaccgttcaa ccggttcaga cgttgaccgt cagaagttgc aggaaaaagt
    21301 acgttcttcg ctcaaccgtc tgcgtcgttt aggcatggtg tggtttatgg gccacgacag
    21361 cagcaagttc cgcattaccg aatcggtgtt ccgcttcgga gccgatgtgc gtgctggcga
    21421 cgatccccgt gaagcacagc gtcgcctgat tcgtgatggc gaagcaatgc cgattgaaaa
    21481 tcatctgcaa ctcaacgatg aaaccgaaga gaatcagcca gatagcggag aggaagaata
    21541 atgattgaac gcggtaaatt tcgctcactg acgctgatta actggaacgg cttttttgcc
    21601 cgaacttttg accttgacga gctggtcacg acgctttctg gcggtaacgg ggcgggtaaa
    21661 tccaccacca tggcggcgtt cgttacggcg ctgatccccg acctgaccct gctgcatttc
    21721 cgtaacacta cggaagccgg ggccaccagc ggttcgcgcg ataaaggtct gcacggtaag
    21781 ctgaaagcgg gtgtctgtta ttcgatgctc gacaccatta actcgcgcca ccagcgcgtg
    21841 gtggtcggtg tgcgtctgca acaggttgcc ggacgcgatc gtaaagtgga tatcaaacca
    21901 tttgccattc agggactgcc gatgtcggtg cagccgacac agctggtgac cgaaactctg
    21961 aacgaacgcc aggcgcgcgt gctgccgctt aacgagctga aagacaagct cgaggcgatg
    22021 gaaggcgtgc agtttaaaca gttcaactcc attaccgatt accactcgct gatgttcgat
    22081 ctgggcatca tcgcgcgtcg tctgcgctcc gcatctgacc gtagcaaatt ctatcgtctg
    22141 atcgaagctt cgctgtatgg cgggatctcc agtgccatta cccgttctct gcgcgactac
    22201 ctgttgccgg aaaacagcgg cgtgcgtaaa gcgttccagg acatggaagc ggcgctgcgt
    22261 gaaaaccgca tgacgctgga agcgattcgt gtcacccaat cggaccgtga cctgtttaag
    22321 catctgatca gcgaagctac caactacgtg gcggcggact acatgcgtca cgccaacgag
    22381 cgccgtgtcc atctcgacaa agcgctggag tttcgtcgcg agctacatac ttcgcgtcag
    22441 caactggcgg ctgagcagta caaacacgtc gatatggcgc gtgagctggc agagcacaac
    22501 ggtgccgaag gcgatctgga agcggattat caggcggcca gcgatcacct gaacctggtg
    22561 caaaccgcac tgcgtcagca ggagaagatc gaacgctacg aagcggatct cgatgagctg
    22621 cagatccgtc tggaagagca aaacgaagtg gtggcagaag ccatcgaacg ccaggaagag
    22681 aacgaggctc gtgcggaagc tgccgaactg gaagtggacg agctgaaaag ccagcttgct
    22741 gactaccagc aggcgctgga cgtccagcaa acgcgcgcga tccagtataa ccaggcgatt
    22801 gctgcgctta atcgtgccaa agaactgtgc catctgccgg acttaaccgc cgacagcgcc
    22861 gccgaatggc tggaaacctt ccaggcgaaa gagctggaag cgactgaaaa aatgctctct
    22921 cttgagcaga aaatgagcat ggcgcaaacc gcgcacagcc agtttgagca ggcttatcag
    22981 ctggtggtgg caatcaacgg cccgctggcg cgtaacgagg cgtgggatgt cgcccgcgaa
    23041 ctgctgcgcg aaggggtcga gcagcgtcac ctggcagatc aggttcaacc gttgcggatg
    23101 cgcttaagtg aactggaaca gcgtctgcgc gagcagcaag aagccgaacg cctgctggca
    23161 gatttctgca aacgtcaggg caagaatttt gatatcgacg aactggaagc cctgcatcag
    23221 gagctggaag cgcgtattgc ctctctttcc gatagcgtgt ctaacgcccg cgaagagcgt
    23281 atggcactgc gccaggagca ggaacagctg cagtctcgca ttcagagttt gatgcagcgt
    23341 gcaccggttt ggctggcagc gcaaaacagt ctcaaccagt tgagtgaaca gtgcggcgaa
    23401 gagttctcct ccagccagga cgtcactgaa tatctgcaac agttgctgga gcgtgagcgc
    23461 gaggcgattg ttgaacgcga tgaagtaggc gcgcgcaaaa acgccgtcga tgaagagatc
    23521 gaacgtttaa gccagcctgg cggctctgaa gatcagcgtc tgaacgcgct ggcggagcgt
    23581 tttggtggtg tgctgctgtc agaaatttat gatgacgtta gcctggaaga tgcgccgtac
    23641 ttctcagcgc tgtatggccc gtcacgccac gccatcgtgg tgccagatct atcacaggta
    23701 actgaacacc tggagggctt gaccgattgc ccggaagatc tctatttgat cgaaggggat
    23761 ccgcagtcat tcgatgacag cgtgttcagc gttgatgagc tggaaaaagc ggtagtggtg
    23821 aaaatcgccg atcgtcagtg gcgttattca cgtttcccgg aagtgccgct gtttggtcgt
    23881 gctgcgcgtg aaagccgtat tgaaagcctc catgccgagc gtgaagtgct ttccgaacgc
    23941 ttcgccacgc tctcctttga cgtacagaaa acccagcgtc tgcatcaggc gttcagccgc
    24001 tttatcggta gtcatctggc ggtggcgttt gagtctgacc cggaagcaga aatccgtcaa
    24061 ctgaacagcc gtcgcgtcga actggagcgg gcgttgagta atcatgaaaa tgataaccag
    24121 cagcagcgta ttcagtttga gcaggcgaaa gaaggcgtta cggcgctgaa ccgcattctg
    24181 ccgcgtctca acctgttggc tgatgacagc ctggcggatc gcgtcgatga aatccgcgaa
    24241 cgtctggatg aagcccagga agccgcgcgt tttgttcagc agtttggcaa tcaactggcg
    24301 aaactggaac cgatcgtttc ggtattgcag agcgacccgg aacagttcga acagttaaaa
    24361 gaagattacg cgtactctca gcagatgcag cgcgatgccc gtcagcaggc gtttgccctg
    24421 acggaagtgg tgcagcgtcg tgcgcacttt agctattctg actcggcaga aatgcttagc
    24481 ggtaacaacg atctcaacga aaaactgcgt gaacgtctgg aacaggcgga agcggagcgt
    24541 acccgcgctc gcgaagcgtt gcgcggtcac gcagcgcagt tgagtcagta caaccaggtg
    24601 ctggcttcgc tgaaaagttc ttacgacacc aaaaaagagc tactcaacga tctgcaacgt
    24661 gaattgcagg atatcggcgt gcgtgctgat agcggggcag aagagcgggc acgtattcgc
    24721 cgtgacgagc tgcatgcgca actgagcaat aaccgttcgc gccgcaacca actggaaaaa
    24781 gcgctcacct tctgcgaagc tgagatggac aacctgaccc gcaaactgcg caagctggag
    24841 cgtgattact ttgagatgcg cgagcaggta gtgaccgcga aagcgggctg gtgtgcagtc
    24901 atgcgcatgg tgaaagataa cggcgttgag cgccgcttac accgtcgtga gctggcttat
    24961 ctctccgctg atgatttgcg ttccatgtcg gataaggcgt taggtgcgct gcgtctggcg
    25021 gtggcggata acgaacatct gcgcgacgtg ctgcgcatgt cggaagatcc gaaacgtccg
    25081 gagcgtaaaa ttcagttctt cgtggcggtt tatcagcatc tgcgtgaacg tattcgtcag
    25141 gatattattc gtaccgatga tccggtggaa gctatcgaac agatggagat cgaacttagc
    25201 cgtctgaccg aagagttaac ctcccgtgaa cagaaactgg cgatcagttc ccgcagcgtg
    25261 gcgaacatca ttcgcaaaac cattcagcgc gaacagaatc gtatccgtat gctcaaccag
    25321 gggttgcaga acgtatcgtt tggtcaggtg aacagcgtac gtctgaacgt gaacgtgcgt
    25381 gaaacgcacg ccatgctgtt ggatgtgctc tctgaacagc acgagcagca tcaggatctg
    25441 tttaacagca accgtctgac cttctcggaa gcgctggcga aattgtatca gcgtcttaat
    25501 ccgcagattg atatggggca gcgcacgccg cagaccattg gcgaagaact gctggattac
    25561 cgcaactatc tggaaatgga agttgaggtt aaccgtggtt ccgatggctg gttgcgcgca
    25621 gagtctggtg cattgtcgac cggtgaggcg attggtaccg gtatgtcgat tctggtgatg
    25681 gtggtacaaa gctgggaaga tgaatctcgc cgcctgcgcg gtaaagatat ctctccttgc
    25741 cgcctgctgt tcctcgatga agcagcgcga ctggatgctc gttctatcgc cacgctgttt
    25801 gaattgtgtg aacgtttgca aatgcagctc atcatcgcag cgccggaaaa tatcagcccg
    25861 gagaaaggca ccacctataa actggtgcgt aaagtcttcc agaataccga acacgttcac
    25921 gtcgtcggcc tgcgaggatt tgcgccgcaa ctccctgaaa cgcttccagg aagtgacgaa
    25981 gcgccttctc aggcgagtta aaattaaggc ggcagcaatg ccgccttttc tttttccgaa
    26041 aactccgttt ctgcactaaa aaagtggcac attacggcgc gcttttcttt aaacttcttt
    26101 acattaggtt atgtaaaaac gtatcggcgt ttatatactg aagataagcc tgatgagtaa
    26161 caggcttgct cgtcatactt tcgtgagtat tggcgttgta caggcaagtc gtaaaataac
    26221 agcctggcta ttcagagtat gataaaaaca gggggcaagg gatgttgctt aatatgatgt
    26281 gtggtcgtcg gctgtcggca atcagtttgt gcctggccgt aacattcgct ccactgttca
    26341 atgcgcaggc cgatgagcct gaagtaatcc ctggcgacag cccggtggct gtcagtgaac
    26401 agggcgaggc actgccgcag gcgcaagcca cggcaataat ggcggggatc ctgccattgc
    26461 ctgaaggtgc ggcagaaaaa gcccgcacgc aaatcgaatc tcaattaccc gcaggttaca
    26521 agccggttta tcttaaccag cttcaactgt tgtatgccgc acgcgatatg caacccatgt
    26581 gggaaaaccg tgatgctgtt aaagccttcc agcaacagct ggcagaggtg gcgattgccg
    26641 gtttccagcc gcagtttaat aaatgggtag agttactgac cgatcctggt gttaacggga
    26701 tggcacgcga cgtggtgctc tctgatgcga tgatgggcta tctccatttc attgcaaata
    26761 ttccggtcaa aggcactcgc tggctatata gcagtaaacc ttatgcgctt tcaacgccgc
    26821 cgctttcggt gattaaccaa tggcagcagg cgctggataa aggtcaattg cctacgtttg
    26881 ttgcaggact ggcaccgcag catccgcaat atgcggcgat gcatgaatcg ttactggcct
    26941 tactcagtga caccaaaccg tggccccaac tgaccggcaa agcaacgttg cgcccagggc
    27001 agtggagtaa cgacgtaccg gcgttgcgcg aaatattgca acgcacaggc atgttggacg
    27061 gggggccgaa aattactcta cctggcgatg acacgccaac tgacgcggta gtcagcccat
    27121 ccgctgttac tgttgaaaca gcagaaacta agccgatgga caagcaaacg acgtctcgta
    27181 gtaaacctgc gcctgccgtt cgtgccgcct acgataatga actggtggaa gccgttaaac
    27241 gttttcaggc atggcaagga ttgggggcag atggtgctat tggcccggca acgcgggact
    27301 ggttaaacgt aacgcccgcc cagcgtgctg gcgtgttggc actcaacatc cagcgattgc
    27361 gcttgctgcc aacagagctt tctaccggga tcatggttaa cattccggcc tattcgctgg
    27421 tctactatca gaacggcaat caggtgctgg attcgcgagt cattgtcggt cgccccgatc
    27481 gcaaaacgcc gatgatgagc agtgcactta acaacgtagt ggtaaacccg ccgtggaacg
    27541 taccaccaac tctggcacgc aaagatattc tgccgaaagt gcgcaacgat ccgggatatc
    27601 tcgaaagcca tggctatacg gtgatgcgcg gctggaacag cagagaagcg attgacccat
    27661 ggcaggttga ctggtctaca atcacggcct cgaatttacc gtttcgcttc cagcaggctc
    27721 caggcccacg gaactcgctg gggcgctata aattcaatat gccgagttca gaggccattt
    27781 atttgcatga cacgccgaac cacaatctgt tcaagcgtga tacacgcgca ttgagctcag
    27841 gctgtgtacg agtgaataaa gcttccgatc tggcgaatat gctgttgcag gatgcaggct
    27901 ggaatgacaa acgtatttct gatgcgctga agcagggtga tacacgttac gtcaatattc
    27961 ggcagtcgat tccggtgaat ctctactacc tgacggcctt tgttggtgca gatggtcgta
    28021 cccagtatcg tacagatatt tacaattatg atctgcctgc gcgatccagc tcgcaaatcg
    28081 tatcgaaagc ggaacaatta atcaggtaaa tgaagtagtt cggggaaatc agttgtcgta
    28141 ataattagca tgattggggg tggttctctg cagcccccgt cactgctggg gttgagtcat
    28201 cttgacgtct gctttacggg cggttaaggt gcctcttgtg cgccagaagt gcatataaac
    28261 gataacattg acctgtagac ttgattatca tggacaaatt cgacgctaat cgccgcaaat
    28321 tgctggcgct tggtggcgtt gcactcggtg ccgccatcct gccgacccct gcgtttgcaa
    28381 cactctctac cccacgcccg cgcattttga cactcaataa tcttcatacc ggagagtcaa
    28441 tcaaagcgga gtttttcgat ggcagaggct atattcagga agaattggca aaacttaacc
    28501 attttttccg cgattaccgc gcgaacaaaa taaagtccat cgacccagga ttattcgacc
    28561 agttgtatcg cctgcaaggg ttattaggca cccgcaaacc ggtgcaactc atttccggtt
    28621 atcgttccat tgataccaac aatgaactac gcgcccgcag ccgtggagta gcgaagaaaa
    28681 gctatcacac taaaggccag gcgatggatt tccatattga aggtatcgcg ttaagcaata
    28741 ttcgcaaagc cgcgttatct atgcgcgcag gtggtgtagg atattaccca cgtagtaact
    28801 ttgtgcatat tgataccggg ccagcacggc actggtagta attgcttaac gaaacagggg
    28861 cagtatgaac tatcgtatta ttccggtcac cgcattctcc cagaactgtt cattgatctg
    28921 gtgtgaacaa acccgactgg ccgcactggt cgatcctggc ggcgatgcgg aaaaaatcaa
    28981 acaggaagtt gatgccagcg gcctgacact gatgcagatc ctgctgacgc atggtcatct
    29041 ggaccacgtt ggcgcagcgg cggaactggc gcaacattac ggcgtgccgg tttttggccc
    29101 ggaaaaagaa gatgagttct ggctgcaagg cttgcctgcg caaagtcgta tgtttggtct
    29161 ggaagagtgt cagccgctga cgccagatcg ttggctgaaa gaaggcgata ccatcagcat
    29221 agggaatgtg actttacagg tgttacattg ccctgggcat acgccgggtc atgtcgtgtt
    29281 ttttgatgat cgggcaaagc tgctgatttc tggcgatgtt attttcaaag gcggagtagg
    29341 gcgcagtgac ttcccgcgtg gcgatcataa tcaactgatt tcttcaatca aagataaatt
    29401 gctgccactg ggggatgacg tgacatttat tccgggtcac ggaccattat ccacacttgg
    29461 ttatgaacgc ctgcataatc ccttcctgca agacgaaatg cccgtctggt aaggcacaca
    29521 taaaaaagcc cgcttttcag cgggcttcat tgtttttaac gcttacagca ctgccacaat
    29581 cgcttcgcac agcggagcca tgttatctgg tgtcatccct gccacattta cgcgaccaga
    29641 agcaacagca tatacgccaa actcttcgcg cagacgcaac acttgttctt ttgtcagacc
    29701 actgaaggag aacatgccgt tctgtttgat gataaagctg aagtcgcggt ttgccccttt
    29761 ttcctgcagc gtattgacga acaactgacg catacgctga atacgctggc gcatatcagt
    29821 cagctcttgt tcccaaatcg cacgtaatgc atcgttgctc aggatggtgg caacaacaga
    29881 agcgccgtgt gctggagggt tagagtagtt agcgcgaatc gccgctttca tttggctgaa
    29941 tgcgcgatca acggtttcac tgtcggcagc aaccagagta caagcgccaa cacgctcgtt
    30001 gtacaggcca aagtttttag agtaggaact ggcaacaatc agctctttat gcatagccgc
    30061 gaaagcgcgc agtccttcag catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa
    30121 gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt gccagtgtct gccattgttc
    30181 cagcgtaggg tcgataccgg ttgggttatg gcagcagcca tggaacagca ctacgtcgcc
    30241 agcctgagct tcgttcaggc tgttaatcag tgcatcgaag tcaagggtgt gattttccgc
    30301 atcataataa gcgtattcac gaacttccag acctgcagag ttaaagacgc tcttatggtt
    30361 cggccagctt gggttgctca cccacacacg cttaacgctg gtattttttg ccaggaagtc
    30421 ggcagccacg cgtagtgcgc cagtgccacc cggagtctgt gccgtgcgag cacgtttgtc
    30481 attgatcagg gcgctacctt taccaaacag cagttcctga gtgcagcgac caaattcagg
    30541 gatgccgtca atgccgaggt aatttttggt ggtttcattt tcgagcagat actgttcagc
    30601 ctttttcacg ctggtcagta ccggggtttt gcccgtctca tctttataga caccaatccc
    30661 gaggttaatt ttgccgggac gttcatcggc acgaaacaga tcggccaggc ccagaatcgg
    30721 gtcggcagga gcggcggtaa tgttctcaaa catgacgagg ttccattatg gttacagaag
    30781 ggaagtccgc tatcagggta acgggagatt tacaaaattc caactattac tgatgaaaac
    30841 gcaggctgtt tttgcaagac gtgagattgc tctggaaggc ataaaaaaaa caggaccaaa
    30901 gtcctgtttt ttcggcattt aacaaagagg tgtgctatta gaactggtaa acgatgccca
    30961 cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc agaatcgatc tggttgatga
    31021 tgtagtcaac ataggtggac atgtttttgt tgaagtagta ggttgcgccc acttcaaagt
    31081 agttcaccag atcaacatca ccgatacctt ctacgtcttt cgctttagat ttggtgtaag
    31141 cgatggacgg acgcagaccg aaatcgaact ggtattgcgc aactaacaga acgtcttgcg
    31201 ttttgttggc gaagccgcct tcaattggcg tagcgttacg ggtttcaccg tagttcgctg
    31261 ccaggtagat gttgttcgcg tcatacttca gaccagttgc ccactgttca gcttttttac
    31321 cctgaccgcg gaattcggct tcctgggcgt cggtacggtc tgctgcaccg taagcaccta
    31381 cgataccaaa gccttcgaat tcgtagctga tagagccacc gacgccgtcg ccgttggaac
    31441 gctcaggaat accagcacgt tcgtttttgc ccaggtactg aacggcaaag ttcaggccgt
    31501 caaccagacc gaagaagttg gagttacgat aggtagcaac gccgcctaca cgaccaacga
    31561 agaagtcatc gctgtatgcc gtgtcaccgc caaactcagg cagcatatcg gtgtaaccca
    31621 gtgcgtcgta aaccacaccg tagttacgac cgtagtcgat agaacccgca tcagcgaatt
    31681 tcagacctgc gaatgccaaa cgagttttgt tacctttctg ggcatcagcg ccttcagagt
    31741 tgttaccctg gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgtg
    31801 tttccccttt aaaaccaaga cgggcataag ttttgtcgcc gtttccgccg taactgttta
    31861 caccattgtc tttagaaaaa taatgcagac cgacagcttt accgtacaga tctactttgt
    31921 tgccatcttt gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga
    31981 cgatcactgc cagaatattg cgcttcatca ttatttatta ccctcatggt ttttttatga
    32041 cacctgccac tgccgtcaat aagttctgtc aataaaaatt tacggaacta ttgatgagag
    32101 tttggtgtct ttatgtgtct gcaggcatct ttccattcaa gctaacgttt cgctaccgtg
    32161 aaagtgctac aaagataaag atttggtttc aaaaagaaaa aatatgtaac taaaagtaaa
    32221 aattaaggaa ctttgtgaa
//
LOCUS       DAZGCQ010000054        32080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000054 DAZGCQ010000000
VERSION     DAZGCQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.054"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..982)
                     /gene="rarD"
                     /locus_tag="ACHK07_002726"
     CDS             complement(92..982)
                     /gene="rarD"
                     /locus_tag="ACHK07_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO8103040.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1034..1501)
                     /gene="yigI"
                     /locus_tag="ACHK07_002727"
     CDS             complement(1034..1501)
                     /gene="yigI"
                     /locus_tag="ACHK07_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO8103041.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1666..2535
                     /gene="pldA"
                     /locus_tag="ACHK07_002728"
     CDS             1666..2535
                     /gene="pldA"
                     /locus_tag="ACHK07_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO8103042.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2668..4497
                     /gene="recQ"
                     /locus_tag="ACHK07_002729"
     CDS             2668..4497
                     /gene="recQ"
                     /locus_tag="ACHK07_002729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO8103043.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4561..5181
                     /gene="rhtC"
                     /locus_tag="ACHK07_002730"
     CDS             4561..5181
                     /gene="rhtC"
                     /locus_tag="ACHK07_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO8103044.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5243..5863)
                     /gene="rhtB"
                     /locus_tag="ACHK07_002731"
     CDS             complement(5243..5863)
                     /gene="rhtB"
                     /locus_tag="ACHK07_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO8103045.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5974..6996
                     /gene="pldB"
                     /locus_tag="ACHK07_002732"
     CDS             5974..6996
                     /gene="pldB"
                     /locus_tag="ACHK07_002732"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO8103046.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7004..7804
                     /gene="yigL"
                     /locus_tag="ACHK07_002733"
     CDS             7004..7804
                     /gene="yigL"
                     /locus_tag="ACHK07_002733"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO8103047.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7880..8779
                     /gene="bioP"
                     /locus_tag="ACHK07_002734"
     CDS             7880..8779
                     /gene="bioP"
                     /locus_tag="ACHK07_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO8103048.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8667..9620)
                     /gene="metR"
                     /locus_tag="ACHK07_002735"
     CDS             complement(8667..9620)
                     /gene="metR"
                     /locus_tag="ACHK07_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO8103049.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9856..12117
                     /gene="metE"
                     /locus_tag="ACHK07_002736"
     CDS             9856..12117
                     /gene="metE"
                     /locus_tag="ACHK07_002736"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO8103050.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12157..12972)
                     /gene="ysgA"
                     /locus_tag="ACHK07_002737"
     CDS             complement(12157..12972)
                     /gene="ysgA"
                     /locus_tag="ACHK07_002737"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO8103051.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGSQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13234..13995
                     /gene="udp"
                     /locus_tag="ACHK07_002738"
     CDS             13234..13995
                     /gene="udp"
                     /locus_tag="ACHK07_002738"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO8103052.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14136..15563
                     /gene="rmuC"
                     /locus_tag="ACHK07_002739"
     CDS             14136..15563
                     /gene="rmuC"
                     /locus_tag="ACHK07_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO8103053.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINSDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15658..16413
                     /gene="ubiE"
                     /locus_tag="ACHK07_002740"
     CDS             15658..16413
                     /gene="ubiE"
                     /locus_tag="ACHK07_002740"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO8103054.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16427..17032
                     /gene="ubiJ"
                     /locus_tag="ACHK07_002741"
     CDS             16427..17032
                     /gene="ubiJ"
                     /locus_tag="ACHK07_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO8103055.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17029..18669
                     /gene="ubiB"
                     /locus_tag="ACHK07_002742"
     CDS             17029..18669
                     /gene="ubiB"
                     /locus_tag="ACHK07_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO8103056.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18748..19017
                     /gene="tatA"
                     /locus_tag="ACHK07_002743"
     CDS             18748..19017
                     /gene="tatA"
                     /locus_tag="ACHK07_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO8103057.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19021..19536
                     /gene="tatB"
                     /locus_tag="ACHK07_002744"
     CDS             19021..19536
                     /gene="tatB"
                     /locus_tag="ACHK07_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO8103058.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19539..20315
                     /gene="tatC"
                     /locus_tag="ACHK07_002745"
     CDS             19539..20315
                     /gene="tatC"
                     /locus_tag="ACHK07_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO8103059.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20357..21139
                     /gene="tatD"
                     /locus_tag="ACHK07_002746"
     CDS             20357..21139
                     /gene="tatD"
                     /locus_tag="ACHK07_002746"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO8103060.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21136..21624)
                     /gene="rfaH"
                     /locus_tag="ACHK07_002747"
     CDS             complement(21136..21624)
                     /gene="rfaH"
                     /locus_tag="ACHK07_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO8103061.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21791..23284
                     /gene="ubiD"
                     /locus_tag="ACHK07_002748"
     CDS             21791..23284
                     /gene="ubiD"
                     /locus_tag="ACHK07_002748"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO8103062.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23330..24031
                     /gene="fre"
                     /locus_tag="ACHK07_002749"
     CDS             23330..24031
                     /gene="fre"
                     /locus_tag="ACHK07_002749"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO8103063.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24223..25386)
                     /gene="fadA"
                     /locus_tag="ACHK07_002750"
     CDS             complement(24223..25386)
                     /gene="fadA"
                     /locus_tag="ACHK07_002750"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO8103064.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25396..27585)
                     /gene="fadB"
                     /locus_tag="ACHK07_002751"
     CDS             complement(25396..27585)
                     /gene="fadB"
                     /locus_tag="ACHK07_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO8103065.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLVEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27775..29106
                     /gene="pepQ"
                     /locus_tag="ACHK07_002752"
     CDS             27775..29106
                     /gene="pepQ"
                     /locus_tag="ACHK07_002752"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO8103066.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29106..29720
                     /gene="yigZ"
                     /locus_tag="ACHK07_002753"
     CDS             29106..29720
                     /gene="yigZ"
                     /locus_tag="ACHK07_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO8103067.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29759..31210
                     /gene="trkH"
                     /locus_tag="ACHK07_002754"
     CDS             29759..31210
                     /gene="trkH"
                     /locus_tag="ACHK07_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO8103068.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFRQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31222..31767
                     /gene="hemG"
                     /locus_tag="ACHK07_002755"
     CDS             31222..31767
                     /gene="hemG"
                     /locus_tag="ACHK07_002755"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO8103069.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7438 a         8176 c         8792 g         7674 t
ORIGIN      
        1 ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct catacaagag
       61 tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc tgcgtataaa
      121 tcgcatccat cacaaaaatt gccagcgcca cccaaataaa ggcgaaagtc accatcttat
      181 cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc gtcgggccaa
      241 tgtactggaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca gtaaaacaca
      301 acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc gacatcgggt
      361 tttgccccat atggctggtt gagctgtcgg caatagcaaa caggtaaatc gccgccacgg
      421 gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca atcttcttgc
      481 gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata ggtagcgaac
      541 caaaagtcca cagctggact aacacgccac atatcgccag aatcaccgcc agccattgca
      601 tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc agcgggttaa
      661 taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag ataaacagta
      721 gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc tgtggcgtct
      781 gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc accataaaga
      841 aaaacgacca gatcacgcga tgcgtcagga tttcatcggc gggcacgtag taaatcaact
      901 tgaagtacgc tggggctata ccccaaataa aataagcggc aagagcgagt aatacgccct
      961 gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca gcagtttacc
     1021 tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata taaagctgtt
     1081 cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc aacaggctac
     1141 tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga agatcaatgg
     1201 tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg tggcgggtta
     1261 aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca atgaccccgc
     1321 cgtgcaaaat gctttgcgcc cagttgccca ccatcattgg ctgattttta aaggccagct
     1381 gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta aatggcatgt
     1441 gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt acggcagaca
     1501 tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt tgttgttggt
     1561 ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa tagcccgcag
     1621 aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg gactctgcag
     1681 ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga ggcaacggtg
     1741 aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat gctgcaggag
     1801 catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta cacccaaacc
     1861 agcgatctga acaaagaagc gattgccagt tacgactggg cggaaaatgc gcgtaaggat
     1921 gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt aggcccgaac
     1981 tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa tagcgaagag
     2041 tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt tgccaccgat
     2101 taccgttttg caggttggac gctgcgcgat gtagagatgg ggtataacca cgactctaac
     2161 gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct gatggcagaa
     2221 aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac tgacgataac
     2281 ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca cctcggtgat
     2341 gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg cgcggagtta
     2401 ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta cagcggctat
     2461 ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtggg ggttatgcta
     2521 aacgatttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag catccaggat
     2581 taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg
     2641 tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc
     2701 ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc gggccaggaa
     2761 gaaattatcg acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc caccggtggc
     2821 ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac cgtggttgtt
     2881 tcaccgctga tttcgttgat gaaagatcag gtggatcaac tgcaagccaa cggcgtggcg
     2941 gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat gacaggctgc
     3001 cgcaccgggc aaattcgtct gctttatatc gccccggaac gcctgatgct ggataacttt
     3061 cttgagcatc tggcgcactg gaatccggtg ttattagccg ttgatgaagc gcactgtatc
     3121 tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt gcgccagcgg
     3181 ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac gcgccaggat
     3241 atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg
     3301 aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat gcgctacgtg
     3361 caggaacagc gcggtaagtc cggcattatc tactgcaaca gccgggcgaa agtagaagac
     3421 accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc cgggctggaa
     3481 aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgacctgca aattgtggtg
     3541 gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt ggtccacttt
     3601 gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg gcgtgatggc
     3661 ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct gcgccgttgt
     3721 ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact caatgcgatg
     3781 ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta ttttggcgaa
     3841 gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac
     3901 ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg
     3961 atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg cgactatggt
     4021 catgacaaac tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc
     4081 gtgatccgcc agctgattca cctcggcctg gtgacgcaaa atattgccca gcattctgcc
     4141 ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt gcaacttgcc
     4201 gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat
     4261 gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagtaat
     4321 gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga acagatgccg
     4381 atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct ggaacgcttt
     4441 ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga agagtagtca
     4501 gcagcataaa aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg agtcaaatgt
     4561 atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat gagtcccggt
     4621 cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga agcgatgatg
     4681 ggcgtgctgg gtattacctg cggcgttatg gtttgggcag ggattgcgtt gcttggcctg
     4741 catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggttgg cggtggcctg
     4801 tatctctgct ggatgggtta ccagatgctg cgcggcgcgc tgaaaaaaga ggcggtttct
     4861 gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca gtttcctgaa aggtttactg
     4921 accaatctcg ctaatccgaa agcgattatc tactttggct cggtgttctc attgtttgtc
     4981 ggtgataacg ttggcactac cgcgcgctgg ggcatttttg cgctgatcat tgtcgaaaca
     5041 ctggcgtggt ttaccgtcgt tgccagcctg tttgccctgc cgcaaatgcg ccgtggttat
     5101 caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg atttggcatt
     5161 catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga taaggcgttt
     5221 acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc gctcccacca
     5281 gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt cctttaatcc
     5341 atagagcaat ccgttgagca agggtggcgt aaccgatcat cacaataata tcgaccacaa
     5401 tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc atgatgaatt
     5461 gcggaaatag cgcggccaga aacacaatac ttttgggatt ggtgagattc acaaaaactg
     5521 cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga tcaattgcgc
     5581 cagcggcgcg ccattgctgt attcccagcc aaatcaagta agccgcgcct gcccacttca
     5641 acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg ccaaccagca
     5701 caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg ccgcgataac
     5761 cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac aggcttaaaa
     5821 tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac tcccggtgtg
     5881 tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg ctaaaaaaaa
     5941 cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa agactgggaa
     6001 acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt ctggcgtcag
     6061 cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt ccgttttcgc
     6121 gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag ctacgtaaaa
     6181 tatgcggaac tggcctacga tttgttccat ttggggtttg atgtcttaat catcgaccat
     6241 cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca tgttaatcgc
     6301 tttaatgatt atgttgatga tctggcggca ttctggcaac aggaggtcca gcccggtccg
     6361 tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac attatttctg
     6421 caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt tgggatcgtg
     6481 attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc gcatccacgt
     6541 ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt tgctatcaac
     6601 gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc tgatgaccca
     6661 acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct ggctggcgaa
     6721 caggtgttag ccggtgcggg tgatgacgcc acgccaacgc ttctcttgca ggctgaagag
     6781 gaacgcgtgg tggataaccg catgcatgac cgtttttgtg aactccgcac cgccgcgggc
     6841 catcctgtcg aagggggacg accgctggta attaaaggtg cgtaccatga gatccttttt
     6901 gaaaaggacg caatgcgctc agttgcgctc cacgccatcg ttgatttttt caacaggcat
     6961 aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc aggttgttgc
     7021 gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt acgccaaaga
     7081 aaccctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg gtcgtcacca
     7141 cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga ttacctccaa
     7201 tggtgcgcgc gttcacgatc tggatggtaa tctgattttt gctcataacc tggatcgcga
     7261 cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta ctaacgttta
     7321 tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct tttttaaaga
     7381 agcggtgttc aaatatgcgc tgtatgagcc tggattactg gagccggaag gtgtcagcaa
     7441 agtgttcttc acctgcgatt cccatgaaca actgctgccg ctggagcagg cgattaacgc
     7501 tcgttggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg aagtgatggc
     7561 gggcggcgtt tcaaaaggcc atgcgctgga agcggtggcg aagaaactgg gctacagcct
     7621 gaaggattgt attgcgtttg gtgacgggat gaacgatgcc gaaatgctgt cgatggcggg
     7681 gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gaccttcatc ccgagctgga
     7741 agtgattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac tctatttatc
     7801 gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg gatactcgtt
     7861 aatcaaagag ttttccattg tggcgctact tatcatcacc acgattttgt gggccttctc
     7921 ctttagtttt tatggcgagt accttgcggg gcacgtcgat agctattttg cggtgctggt
     7981 gcgcgttggc ctggcggcac tcgtttttct gccgtttctg cgtacccgtg gcaatagcct
     8041 gaaaacagtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga tgtatatgct
     8101 gagtttccgc gcttatctct acctgacggt ttccgaactg ctgctgttca ccgtactgac
     8161 gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc gctggggata
     8221 tgcttttagt gccttgttgg cggtgattgg tgccgggatt attcgctatg atcaggtcac
     8281 cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt ttgccatcgg
     8341 catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccacagcata acgcctttgc
     8401 gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc tgggaaatgc
     8461 gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg gcgtggtggc
     8521 ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg ccggaacgct
     8581 gggaattatg aataatatgc acgttccggc agggctgctg gtaaacctgg ctatctggca
     8641 ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg cctcactgtg
     8701 ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc gcaggcgtga
     8761 ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc atcgcgcacg
     8821 gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac cagaccctgg
     8881 cgctcaaaac tctctactac ccaatgcggc agggcggcaa tacccatccg cgcggcaacc
     8941 atctgaatca acaataaggt gttatcgacg cttttcagtg acgggcttac gcctgccggc
     9001 tgaagaaaat gccgccagac atccagtcgg ctacgctgca ccggataaat caacagcgtc
     9061 tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccaatggatg gtcaggtgct
     9121 aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact gcgcggcagg
     9181 atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg cgggtcaaac
     9241 gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa attttctaac
     9301 gcaggcgtca gccactgaat acagctatgg cactcaatgg caatgcgcag acgcgtctgc
     9361 tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg gtttgccagt
     9421 tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg cacaaagagc
     9481 cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga ttgcgtctga
     9541 tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc gcaacgcttg tagcgttttc
     9601 aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt gcgcttgagg
     9661 aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa atccttcgtc
     9721 ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact tcagtaagct
     9781 cccggggatg aataaacttg ccgccttccc taaattcaaa atccatagga tttacatata
     9841 attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg cgttggcctg
     9901 cgtcgcgagc tgaaaaaagc gcaagagagt tattgggcgg ggaactccac gcgtgaagaa
     9961 ctgctggcgg tagggcgtga attgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
    10021 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac caccagtctg
    10081 ctgctgggta acgttccggc gcgtcatcag aacaaagatg gttcggtaga tatcgacacc
    10141 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    10201 accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa aggccaacag
    10261 ttcaaactga cctggacgca gctgctggac gaagtggacg aggcgctggc gctgggccac
    10321 aaggtgaaac ctgtgctgct ggggccgatt acctggctgt ggctggggaa agtgaaaggt
    10381 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    10441 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    10501 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    10561 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    10621 gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagatga cgttgttgaa
    10681 ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa tggtcgtaac
    10741 gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    10801 gatttgtggg tggcatcatc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    10861 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    10921 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    10981 gccccaattc aggcgcgtcg tcactctact cgtgtacata acccggcagt agaaaagcgt
    11041 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgccgaa
    11101 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    11161 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaat
    11221 aactaccgca cgggcattgc ggaacatatc aggcaggcca ttgttgaaca ggaacgtttg
    11281 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atattttggc
    11341 gagcacctcg acgggtttgt ctttacgcaa aacggttggg tacagagcta tggttcccgc
    11401 tgcgtgaagc caccgattgt cattggtgat attagccgcc cggcaccgat taccgtggag
    11461 tgggcgaagt atgcgcaatc tctgactgat aaaccggtga aagggatgct gactggcccg
    11521 gtgaccattc tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    11581 caaattgcgc tggcgctgcg tgatgaagtg gcggacctgg aagccgcagg aattggcatc
    11641 atccagattg atgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat
    11701 gcatatctcc agtggggcgt ggaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    11761 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    11821 gcactggatg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    11881 tcgtttgaag agtttgatta tccgaatgaa atcggtccag gcgtctatga cattcactcg
    11941 ccaaacgtac cgagcgtaga atggattgaa gccttgctga agaaagcagc acaacgcatt
    12001 ccggcagagc gcctgtgggt taacccggac tgtggtctga aaacgcgcgg ctggccagaa
    12061 acccgcgccg cgctggcgaa catggtacag gcggcgcaga atttgcgtcg ggggtaaaat
    12121 ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct tcccaccata
    12181 ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg catgatagct
    12241 cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa tctctgcttt
    12301 cgcattagca gcccgcagcg cctggcgcat ggtttcaacg ctctcctgcg gaatgctgtt
    12361 atcctgactg ccatataagc cgagaaccgg cgcgttaaga tcggttgcga tatcaacagg
    12421 ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg ccactgcggc
    12481 ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc agcagaatcc
    12541 ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cgacatgatc
    12601 gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa gcaacgtggg
    12661 gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg cgatagccag
    12721 atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat
    12781 ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta
    12841 agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc
    12901 cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc cagattgttg
    12961 tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt
    13021 cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg
    13081 aagagtgatt tatgtgattt gaatcacttt tggtgggtaa atttatgcaa cgcatttgcg
    13141 tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc
    13201 ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc
    13261 ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat
    13321 cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa
    13381 ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc
    13441 ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc
    13501 ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga tgtcctggtt
    13561 accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg
    13621 gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc cattggagcg
    13681 acaactcacg ttggcgtgac agcttcttct gataccttct acccaggtca ggaacgttac
    13741 gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg
    13801 atgggcgtaa tgaactatga aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag
    13861 ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca agagatcccg
    13921 aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt ggaagcggcg
    13981 cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc cttttgtatt
    14041 tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag cacaattcta
    14101 ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc atggtttacg
    14161 cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat caacatgcgc
    14221 agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta agcgcggcaa
    14281 aacaacaaat tacccaaagc gagcactggc gtgcagagtg cgagttactc aataacgaag
    14341 tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa gtaaccacgc
    14401 ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt aacagcgagc
    14461 agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac agcaatcgcc
    14521 gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta cgtgaacaac
    14581 tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa gaacgccata
    14641 ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc caggaagcga
    14701 tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg ggcgaggtag
    14761 tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat gaaacccagg
    14821 tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg cgcctgccgc
    14881 agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa cgctatttta
    14941 acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg tcggtgcgta
    15001 accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg cgaactctgg
    15061 attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt gaccgccagc
    15121 cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg actacgctgc
    15181 tggtggcgct gcgcactatc gccaacctgt ggcgttatga gcatcaaagc cgcaacgccc
    15241 agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc atcgatgaca
    15301 tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ttatcggcag gcaatgaaaa
    15361 aactctcttc agggcgcgga aatgtgctgg cgcaggcaga agcgtttcgc ggtttaggag
    15421 tagaaattaa acgcgagatt aattcggatt tggctgaaca ggcggtgagc caggatgaag
    15481 agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa cgcgatgatg
    15541 aatataatca gcagtcgcgc tagcccattg ggagtagtta agccgggtag aaatctaggg
    15601 catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg cattgagatg
    15661 gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa ggaacaaaaa
    15721 gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt catgaatgat
    15781 ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg cagcggcgta
    15841 cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac agcgaaattc
    15901 tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga atccatgctc
    15961 aaaatgggcc gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga gtatgttcag
    16021 gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac catttcgttt
    16081 ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg cgtgctgaaa
    16141 cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc gctgagcaaa
    16201 gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt cgcgaacgac
    16261 gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca ggataccctg
    16321 aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct gacggcaggg
    16381 gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc cttttaaacc
    16441 tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct cacccgcgct
    16501 gaaaacggcc cgctcgcgtc tgttgggcaa agtgctgcgc gtggaggtaa aaggcttttc
    16561 gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg aatgggcagg
    16621 cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc gcgatcgcca
    16681 gcagcttgcc gccctgattc gcagtggtga gctggaagtg cagggcgata ttcaggtggt
    16741 gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac tgctggcccc
    16801 ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag gcgcaaagtt
    16861 cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta ctgaagagtg
    16921 gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaggagacgg ctgccgtcga
    16981 gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat gacgccaggt
    17041 gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact tgatgaactg
    17101 atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt attctggatg
    17161 ccaaatcggc ataaagacaa acctttaggt gagcgactac gactggccct gcaagaactg
    17221 gggccggtat ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct ttttccgccg
    17281 catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga tggcaagctg
    17341 gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg gtttgacgat
    17401 tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc gcgattgaaa
    17461 tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc ggttattaaa
    17521 gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct gccggatggt
    17581 cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agacattgat tgatgaactg
    17641 aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga agacagcccg
    17701 atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat ggtgatggag
    17761 cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg caccaacatg
    17821 aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg cgacagcttt
    17881 ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc ggaaaacccg
    17941 aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga taaacgctat
    18001 ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc agagctacac
    18061 gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt tgctattcgt
    18121 acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg acatgtactg
    18181 ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca actggtgtta
    18241 ctccagaaaa ccctgctcta cgtcgaaggg gtaggacgcc agctttatcc gcaactcgat
    18301 ttatggaaaa cggcgaagcc tttcctggag tcgtggatta aagatcaggt cggtattcct
    18361 gcgctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat gccagaactg
    18421 cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag tgttgataag
    18481 attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta ttttctcgga
    18541 attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc tgaatggggg
    18601 ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg ttggcgcaaa
    18661 acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg gctttgttta
    18721 atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca gttattgatt
    18781 attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat cggttccgat
    18841 cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc aaagcaggat
    18901 aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca ggcggatacg
    18961 aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca ggtgtaatcc
    19021 gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct cgtcgttctg
    19081 gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggatccg cgcgttgcgt
    19141 tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca ggagtttcag
    19201 gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga actgaaagcg
    19261 tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt tgcaaacgat
    19321 cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa agataatgaa
    19381 gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc gccggaacag
    19441 aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga accgaaaacc
    19501 gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa gatactcaac
    19561 cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt atcgcggtga
    19621 tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacctg gtatccgcgc
    19681 cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg gcctcgccgt
    19741 tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg ccggtgattc
    19801 tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt cgcctggtgg
    19861 tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcgttc gcctactttg
    19921 tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg gtgcaggtat
    19981 ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg tttggtgtct
    20041 cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc tcgccagaag
    20101 acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg atgttgctga
    20161 cgccgccgga tgtcttctcg caaacgctgt tggcgatccc gatgtactgt ctgtttgaaa
    20221 tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa gaggaaaacg
    20281 acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc agggcggttg
    20341 tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc aatttgcgaa
    20401 agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc tactcatcac
    20461 cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt attcatcctg
    20521 ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg cgactgaaga
    20581 agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat gtggtctcga
    20641 ctttaaccgc aacttttcga cgccggaaga gcaggaacgc gcttttgttg cccagctacg
    20701 cattgccgca gatttaaaca tgccggtatt tatgcactgt cgcgatgccc acgagcggtt
    20761 tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc attgctttac
    20821 cggcacacgc gaagagatgc aggcgtgcgt ggcgcatgga atttatatcg gcattaccgg
    20881 ttgggtttgc gatgaacgac gcggactgga gctgcgggaa cttttgccgt tgattccggc
    20941 ggaaaaatta ctgatcgaaa ctgatgcgcc gtatctgctc cctcgcgatc tcacgccaaa
    21001 gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac gtattgcgca
    21061 ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgctaatg tcaaaacact
    21121 gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt gcttaatctc
    21181 tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt cggtgaaaat
    21241 ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg gataaggggt
    21301 tgccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatca ccgccgatgg
    21361 gactatcgct ggcgacgcgc caaagcgcac gaagtggctg acaccgcggg tcgcgttgat
    21421 agtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttgg ggaacaatgg
    21481 ctcactgact gcagtacgtt tgccacgcac gattttttcc agggtgatca tcggtgccag
    21541 gcaattcaca gcctgtcttt cgaggtgttc ctgggcacgt tgaagttgcc cgcgcttgca
    21601 gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg cgcaaggata
    21661 gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg tcagagttca
    21721 cgctaattta acaaatttac agcatcgcaa agatgaacgc cgtataatgg gcgtagatta
    21781 agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcttga cgctgcttga
    21841 acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg aaatcactga
    21901 aattgctgac cgcactttgc gtgccggtgg gcctgcgctg ttgttcgaaa accctaaagg
    21961 ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg cgatgggcat
    22021 ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt ttctgaaaga
    22081 gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt ttaagcaagt
    22141 attgaacatg ccgacaaagc ggctgcgtgg tgcgccctgc caacaaaaaa tcgtctctgg
    22201 cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag atgccgcgcc
    22261 gctgattacc tgggggctga cagtgacgcg cggcccgcat aaagagcggc agaatctggg
    22321 catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc tgtcgcatcg
    22381 cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac gtttcccggt
    22441 ttctgtggcg ctgggtgccg atcccgccac gattctcggt gcagtcaccc ccgttccgga
    22501 tacactttca gagtatgcgt ttgccggatt gctgcgcggt accaagaccg aagtggtgaa
    22561 gtgtatctcc aatgaccttg aagtgcccgc cagtgcggag attgtgctgg aagggtatat
    22621 cgaacaaggt gaaacggcgc cggaagggcc gtatggcgac cacaccggtt actataacga
    22681 agtcgatagt ttcccggtat ttaccgtgac gcatattacc cagcgtgaag atgcgattta
    22741 ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg tggcgttgaa
    22801 cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt tttacctgcc
    22861 gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt acgccggaca
    22921 cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt acactaaatt
    22981 tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga tttgggcgat
    23041 taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc ctattgatta
    23101 tctggatttt gcctcgcctg tctccgggct gggttcaaaa atggggctgg atgccacgaa
    23161 taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag atccagatgt
    23221 tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg gtaaaagcgc
    23281 ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca tgacaacctt
    23341 aagctgtaaa gtgacctcgg tagaagctat cacggatacc gtatatcgtg tccgcatcgt
    23401 gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga tggatgagcg
    23461 cgacaaacgt ccgttctcaa tggcttcgac gccggatgaa aaagggttta tcgagctgca
    23521 tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca tcctcaaaga
    23581 tcatcaaatc gtggtcgata ttccccacgg agaagcgtgg ctgcgcgatg atgaagagcg
    23641 tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga ttttgctgac
    23701 agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc gtgaagagca
    23761 gcatctgtat gatctctgcg agcttgaggc gctttcgttg aagcatcctg gtctgcaagt
    23821 ggtgccggtg gtagaacaac cggaagcggg ctggcgtggg cgtactggca ccgtgttaac
    23881 ggcggtattg caggatcacg gtacgctggc agagcatgat atctatattg ccggacgttt
    23941 tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc gggaagatcg
    24001 cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc ctgacaggcg
    24061 ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc atccggcatt
    24121 cggcacacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt ggtgccggat
    24181 cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc gctcaaacac
    24241 cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact gaacgtcttt
    24301 gtgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac ccagcggatg
    24361 acccagcgcg atcgcgccac cgttgaggtt gatcttctcg tcaatctgct caattagtcc
    24421 cagatcttta atacatggca ggatctgcgc ggcaaaggct tcattcattt caaacacgcc
    24481 gatatcactg acagaaagcc ccgctttttt cagcgccagc ttcgaggccg gaaccgggcc
    24541 gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac gagcgcgcgg
    24601 cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag ctgcgccatc
    24661 ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa acgccggacg
    24721 cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt aattaaactg
    24781 cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt taaatgcggc
    24841 cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat cctgcatttc
    24901 acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca tgcccgccgc
    24961 tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca tcggcacatg
    25021 gcccatatgc tccacgccgc caaccagaca tgcctgcgca tcgccggtca tgatcattcg
    25081 tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacag taaccgccgg
    25141 gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatattaa aaccctgctc
    25201 cagcgtctgc tgcacacaac cccagtaaat atcgtcgagg gccgccgctt ccagcgccgg
    25261 gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttctgcac gcacgttacg
    25321 aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa tgacaacctg
    25381 ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg gctcaaccgg
    25441 gggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca cttcgtacag
    25501 cgggccgagg tgctgatatt gctgtgccat atcgaggtat ttcgcgctgc cgagggtgtc
    25561 cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga ccagcgccat
    25621 atccgcttct gccggagtgg cgataatgcc ttcttccaga caacgcacca cttcgttgac
    25681 catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct tcggctggct
    25741 cacttctact agcaggtcgt caacagcggc gtcttcttct ttcttcggct tacctttgct
    25801 gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt tggcatcaaa
    25861 cagcgcgtcg atggcatcgc gataatcttt ctgcatccgc tgcgggaagc ctgctgccat
    25921 caccgcctga gcgtgatgcg cggtgtcaat gcccacgaca tccagcagat atgccggacc
    25981 catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat ccgcgccgtc
    26041 gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttga cgaagaagcc
    26101 ggggcaatcg ttaaccacaa tcggcgtttt acccatcttg ctcgcccagg cgacgacttt
    26161 cgcaatagtt tcgtcggagc ttttctcgcc gcgaataatt tccaccaacg gcattcggtg
    26221 gaccgggtta aagaagtgca tcccgcagaa gttttccgga cgttccagcg cgttggccag
    26281 ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatccgggc gtactttctg
    26341 ctcggtttct gccagtacgg cttttttcac tttcgggttt tcgacaaccg cttctaccac
    26401 aacatccacg cggtcaaacc cggcgtagtc gagcgttggg tggattgtag agatcacgcc
    26461 agccagtttc agaccatcga tcttgccgcg ctcaagctgc ttgttcagca gtttcgcggc
    26521 ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa ccggcacgcc
    26581 tttccacgca gactggtaag cgatgccgcc gcccataatg cctgcgccca gcacagcggc
    26641 ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta catattgatc
    26701 gttaaggaat atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg ggacaaaact
    26761 tttgttttcc aggtttagag cttcttcacg accaaaacgg gccgcagctt caatggtttt
    26821 tactgcggtg atgggggccg gataatgttt ccccgccgtt tgtgcgacca tccctttagc
    26881 gatggtgaag ctcatggtag cttcaatctt gctcagtttt agcggttcca gcttcggctg
    26941 acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg ccatcgcccc
    27001 ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccaattttca gcgcctggtc
    27061 cgcgccgaca tctttaccgg cggcaatgat ttccagcgca ctgtcagcgc ccagcatacg
    27121 tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt ccggcagacc
    27181 gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt cgcagccgcc
    27241 acccagcgcg tagccgttga cggcagcaat ggtcggcacc ggcagatctt ccaggcgatt
    27301 aaacacgcta ttggcaaaat gcagccactg gcttaactgt tcttcaggaa cgaggaacag
    27361 ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg aacgcagcag
    27421 cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga ggctggcgac
    27481 ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt cggcaatgcc
    27541 atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag tctcctgaat
    27601 ccgcaaggtg atctggtcgt accagatgag tcgaagtgtg tattttgtgt taaaaatatg
    27661 caaacaaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca cgctctggat
    27721 gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta aaaaatggaa
    27781 tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac tcgcgatgcg
    27841 ctggcgcgct tcaagctgga tgcgttactt attcactccg gcgagctgtt caatgttttt
    27901 ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg ggtgccggta
    27961 actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa actgtggttt
    28021 tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt ctggactgaa
    28081 gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct gcctgctgcg
    28141 cgcggcaata tcggttatat cggtccggtg ccggagcgtg cgctgcaact gggtattgag
    28201 gccagcaaca tcaacccgaa aggggttatc gactacctgc attactatcg ctccttcaaa
    28261 accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa cggtcatcgt
    28321 gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaacat tgcctatctg
    28381 accgcgactg gtcatcgtga taccgacgta ccttacagca acattgtggc gcttaacgaa
    28441 cacgctgcgg tgctgcatta caccaaactg gatcatcagg cgccggaaga gatgcgcagc
    28501 ttcctgctgg atgccggggc cgaatataac ggctatgccg ctgacctgac ccgtacctgg
    28561 tcggcaaaaa gtgacaacga ttacgcacag ctggtgaaag acgtaaatga tgaacaactg
    28621 gcgctgatcg cgaccatgaa agctggcgtt agctatgtgg attaccacat ccagttccat
    28681 cagcgcatcg ccaaattgct gcgtaaacat caaatcatca ccgatatgag tgaagaagcg
    28741 atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtatcggcca tccgctgggc
    28801 ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacgca cctcgcggca
    28861 ccggcaaaat atccgtacct gcgctgcacc cgtattctcc agccgggcat ggtgttaacc
    28921 atcgaaccgg gtatctactt cattgaatcg ctactggcac cgtggcgtga agggcagttc
    28981 agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg cattcgtatc
    29041 gaagacaacg tggtgatcca cgaaaataac gtggaaaaca tgacccggga tctgaaactg
    29101 gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa gagatcaaaa
    29161 aaagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg aaagcgtttg
    29221 ttgaatcggt gcgggcagaa catcccgatg cccgccacca ttgtgtggcg tgggtggcgg
    29281 gtgcgccgga tgattcacaa cagttgggtt tctctgacga cggggaaccg gcgggaacgg
    29341 caggtaaacc gatgctcgcc caactgatgg gcagcggcgt cggggaaatt accgccgtgg
    29401 tggtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa gcgtatggcg
    29461 gcggcgtgaa tcaggcgctg cggcagctaa cgacccaacg caagacgcca ttaaccgaat
    29521 atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg gggcagtgtg
    29581 acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg gcgcttccgg
    29641 cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt tcattgcaat
    29701 tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag cggcagagat
    29761 gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt cagggaccat
    29821 gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg cttttaccca
    29881 gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc gcaaagagaa
    29941 gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttctgga ccgtgctggg
    30001 tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga ttaccgatgc
    30061 gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg tggggctgga
    30121 ttcgctccct cacgccatcc tcttttatcg ccagatgctg caatggtttg gcgggatggg
    30181 gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga tgcagctcta
    30241 tcgtgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa ttgcggaaac
    30301 ggcgaaaacc ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc tggcgttgtg
    30361 gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta tcgctattgg
    30421 cggcttctcg acacatgatg ccagtatcgg ttatttcgat agcccgacta ttaacactat
    30481 cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt cactgttaag
    30541 tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta tcggcgtaca
    30601 gtttacgctg gtggttattt gtaccctcgt actgtggttt cataatgtct acagttcggc
    30661 gctgatgaca attaaccagg cgtttttcca ggtggtgtcg atggcgacaa ccgccgggtt
    30721 tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt tatgttcagc
    30781 ttttatcggc ggttgtgccg ggtcaacggg cggtggcctg aaagtgatcc gcatcctgct
    30841 gctgtttagg caggggaacc gtgagctgaa acgactggtg catccgaacg ccgtctatag
    30901 cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgtct ggggattttt
    30961 ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca cgggcgtgga
    31021 tgacttttct gcctttgcct cggttgttgc gacattgaat aacctggggc cagggcttgg
    31081 cgtggttgct gataacttta ccagcatgaa cccggtggct aaatggatcc tgattgccaa
    31141 catgctgttt ggccgtctcg aggtctttac attgctggtg ctctttaccc cgactttctg
    31201 gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag ggacggacaa
    31261 acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat ccaggcggat
    31321 gtcgccaatg tgcaccgcat tgaagaacca cagtgggaaa actacgaccg tgtggtcatt
    31381 ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt caaaaaacat
    31441 gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt ggcgcgcaaa
    31501 ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat gaactcgcaa
    31561 tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg ttatcgctgg
    31621 tacgaccgtt ttatgatcaa gctgattatg aagatgtcag gcggtgaaac ggatacgcgc
    31681 aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga aatcgcccat
    31741 ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg gtcagaaaat
    31801 tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac
    31861 ggaacaacgg caaacacgcc gccgggtcag cgggattctc ctgagaattc cggcagagaa
    31921 agcaaaaata aatgcttgac tctgtagcgg gaaagtgtat tatgcacacc ccgcgccgct
    31981 gagaaaaagc gaagcggcac tgctctttaa caatttatca gacaatctgt gtgggcactc
    32041 gaagatacgg attcttaacg tcgcaagacg aaaaatgaat
//
LOCUS       DAZGCQ010000055        30727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000055 DAZGCQ010000000
VERSION     DAZGCQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.055"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            452..2041
                     /gene="purH"
                     /locus_tag="ACHK07_002756"
     CDS             452..2041
                     /gene="purH"
                     /locus_tag="ACHK07_002756"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO8103070.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2053..3342
                     /gene="purD"
                     /locus_tag="ACHK07_002757"
     CDS             2053..3342
                     /gene="purD"
                     /locus_tag="ACHK07_002757"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO8103071.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHK07_002758"
     CDS             complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHK07_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO8103072.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHK07_002759"
     CDS             complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHK07_002759"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO8103073.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            6275..6700
                     /gene="zraP"
                     /locus_tag="ACHK07_002760"
     CDS             6275..6700
                     /gene="zraP"
                     /locus_tag="ACHK07_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO8103074.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHK07_002761"
     CDS             complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHK07_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO8103075.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHK07_002762"
     CDS             complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHK07_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO8103076.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHK07_002763"
     CDS             complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHK07_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO8103077.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHK07_002764"
     CDS             complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHK07_002764"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO8103078.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHK07_002765"
     CDS             complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHK07_002765"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO8103079.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGDVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHK07_002766"
     CDS             complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHK07_002766"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO8103080.1"
                     /translation="MDRIIEKLDHGCWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            11155..11631
                     /gene="rsd"
                     /locus_tag="ACHK07_002767"
     CDS             11155..11631
                     /gene="rsd"
                     /locus_tag="ACHK07_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO8103081.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     regulatory      11683..11783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11864..13759
                     /gene="thiC"
                     /locus_tag="ACHK07_002768"
     CDS             11864..13759
                     /gene="thiC"
                     /locus_tag="ACHK07_002768"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO8103082.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGAHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     gene            13759..14394
                     /gene="thiE"
                     /locus_tag="ACHK07_002769"
     CDS             13759..14394
                     /gene="thiE"
                     /locus_tag="ACHK07_002769"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO8103083.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQTADWRLATAQLLEIAGVGDE"
     gene            14387..15142
                     /gene="thiF"
                     /locus_tag="ACHK07_002770"
                     /pseudo
     CDS             14387..15142
                     /gene="thiF"
                     /locus_tag="ACHK07_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            15126..15326
                     /gene="thiS"
                     /locus_tag="ACHK07_002771"
     CDS             15126..15326
                     /gene="thiS"
                     /locus_tag="ACHK07_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO8103084.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            15328..16098
                     /gene="thiG"
                     /locus_tag="ACHK07_002772"
     CDS             15328..16098
                     /gene="thiG"
                     /locus_tag="ACHK07_002772"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO8103085.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            16095..17228
                     /gene="thiH"
                     /locus_tag="ACHK07_002773"
     CDS             16095..17228
                     /gene="thiH"
                     /locus_tag="ACHK07_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO8103086.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHK07_002774"
                     /pseudo
     CDS             complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHK07_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHK07_002775"
     CDS             complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHK07_002775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO8103087.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHK07_002776"
     CDS             complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHK07_002776"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO8103088.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHK07_002777"
     CDS             complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHK07_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO8103089.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHK07_002778"
     CDS             complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHK07_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO8103090.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHK07_002779"
     CDS             complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHK07_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO8103091.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHK07_002780"
     CDS             complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHK07_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO8103092.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHK07_002781"
     CDS             complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHK07_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO8103093.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHK07_002782"
     CDS             complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHK07_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO8103094.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
BASE COUNT         7116 a         8396 c         7973 g         7242 t
ORIGIN      
        1 cactgtcgcg aggtggcgta tattacgctt tcctctttca gagtcaaccc tgaatttcag
       61 ggtttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacatcc gccgtgtcga
      121 tggaggcgca ttatagggag ttctccgcag gtcgcaatag aaaaattgca taaaaataac
      181 tgactgctgc attccccagc aaaaccccgc tttatacctt tttacacaca gagttatcca
      241 caatcatcag cgttattttt gtatttttca cacggtaacc actgtcaaaa ttgtgatcac
      301 cattgaaaga gaaaaattcg cgagcgttgc gcaaacgttt tcgttacaat gcgggcgaaa
      361 aataaggatg ccccgttagg ggcgttagct gagtttttcg cgaaaaattc agctaacgct
      421 ctctgtaata gtcaaatcca ggggatttac catgcaacaa cgtcgtccag tccgccgcgc
      481 tctgctcagt gtttctgaca aagccggtat cgtcgaattc gcccaggcac tttccgcacg
      541 cggtgtggag ctgctgtcta cagggggcac tgcccgtctg ttagcagaaa aaggtctgcc
      601 ggtaaccgaa gtttccgatt acaccggttt cccggagatg atggatggac gcgtgaagac
      661 cctgcatccg aaagtacatg gtggcattct gggccgtcgc ggccaggacg atgccattat
      721 ggaagaacat cagatccagc ctatcgatat ggtggttgtt aacctgtatc cgttcgccca
      781 gacagtggcc cgtgaaggtt gctcgctgga agatgcggtt gagaacatcg atatcggcgg
      841 cccaacgatg gtgcgctccg ccgccaagaa ccataaagat gtcgcaatcg tggtgaagag
      901 cagcgactac gacgccatta ttaaagagat ggatgacaac gaaggatcgc tgacgcttgc
      961 aacccgtttc gacctcgcca tcaaagcctt cgaacacact gccgcctacg acagcatgat
     1021 tgccaactac ttcggcagca tggttccggc ttaccacggt gaaagcaaag aagccgccgg
     1081 tcgcttcccg cgcacgctga acctgaactt cattaagaag caggatatgc gctacggcga
     1141 gaacagccac cagcaggctg ccttctatat agaagagaat gtgaaagaag cctccgttgc
     1201 taccgcaacc caggttcagg gtaaagccct ctcttataac aacatcgccg ataccgatgc
     1261 ggcgctggag tgcgtgaaag agttcgccga gccggcatgt gtgattgtga agcacgccaa
     1321 cccttgcggc gtggctatcg gcaattccat tcttgatgct tacgatcgcg cgtacaaaac
     1381 cgacccgacc tccgcattcg gcggcatcat tgcctttaac cgcgagctgg atgcggaaac
     1441 cgcacaggcc atcatttctc gtcagtttgt tgaagtgatt attgcgccgt ccgccagcga
     1501 agaagccctg aaaatcaccg ccgccaaaca gaatgtacgc gttctgacct gcggtcagtg
     1561 gggcgagcgt gttccgggcc tcgatttcaa acgcgtgaac ggcggtctgc tggttcagga
     1621 tcgcgatctg ggcatggtcg gtgcggaaga actgcgcgtc gtcacccaac gtcagccgac
     1681 cgaacaggaa ctgcgtgatg cgctgttctg ctggaaagtg gcgaagttcg tgaaatccaa
     1741 tgctatcgtc tatgccaaaa acaatatgac catcggtatt ggcgcgggcc agatgagccg
     1801 cgtgtactcc gcgaaaatcg ccggtattaa agctgccgat gaaggcctgg aagtgaaagg
     1861 ttcctcgatg gcttctgacg cgttcttccc gttccgcgac ggtattgatg ccgccgcggc
     1921 tgcgggcgtg acctgtgtaa tccagcctgg cggttctatc cgtgatgacg aagtgattgc
     1981 cgccgccgac gagcacggta ttgcgatgct cttcaccgac atgcgccact tccgccatta
     2041 atggagcaat agatgaaagt attagtgatt ggtaacggcg ggcgcgagca cgcgctggcc
     2101 tggaaagcgg cccagtcgcc gctggttgag actgtttttg ttgctccggg taatgcaggc
     2161 actgcgctgg aacccgcgct gcaaaacgtt gctatcggcg tgaccgatat cccggcgctg
     2221 ttggatttcg cacaaaacga aaagattgat ctgaccatcg tcggcccgga agcgccgctg
     2281 gtgaaaggcg tggtcgatac cttccgcgct gccgggctga aaatcttcgg cccaaccgca
     2341 ggtgcggccc aactggaagg ctcaaaagcg tttaccaaag atttcctggc ccgccataag
     2401 atccctacgg cggaatacca gaacttcacc gaggtagaac ctgcgctggc gtatctgcgt
     2461 gagaaaggcg cgccaatcgt cattaaagcg gacggtctgg ctgccgggaa aggcgttatc
     2521 gtggcgatga cgctggaaga agcggaagcg gctgttcacg atatgctggc gggcaacgct
     2581 tttggcgacg cgggtcatcg catcgttatc gaagagttcc tcgatggcga agaagcgagc
     2641 tttatcgtga tggtggacgg cgagcatgtg ctgccgatgg ctaccagcca ggatcacaaa
     2701 cgcgtaggcg ataaagatac cggaccaaac acgggcggga tgggcgctta ctcccccgcg
     2761 ccggtagtaa ccgatgaagt tcatcagcgc accatggaac gtattatctg gccaaccgtg
     2821 aaaggcatgg cggcggaagg caacacctac accggttttc tctacgcggg cctgatgatc
     2881 gacaaacagg gcaatccgaa ggttattgaa tttaactgcc gctttggcga tccggaaacc
     2941 cagccgatta tgctgcgcat gaagtccgat ctggttgagc tctgcctggc ggcctgtgaa
     3001 ggcaaactgg acgagaaaac gtcagagtgg gatgaacgtg cttctctcgg cgtggtgatg
     3061 gctgcgggtg gatatccggg cgattaccgc accggcgatg tgatccacgg cctgccgctg
     3121 gaagaagtgg ctgacggcaa agtgttccac gcgggcacga aactggcgga taacgagcag
     3181 gtagtgacca gcggcgggcg cgtactgtgc gtcaccgcgc tgggtcatac cgtggcagaa
     3241 gcgcagaaac gcgcttatgc cttaatgacc gatattcact gggacgactg cttctgccgg
     3301 aaagatatcg gctggcgcgc tatcgaacgc gagcagaact aacgcgacag ttttgccaat
     3361 agcgttttgc gcgtgatccc taactgacgg gcggcttcgg ttttgttgcc gcccgttttc
     3421 tccagtgccg ccagaatcac ttctttttcg acttccacca gcggctgaat atcctgactt
     3481 tgccccagcg ggatcggcgt accggcaatc gccagcggca gctcgcgttc ggaaatatat
     3541 tccccggtca gcagcaccac cgcccgttcc accgcgtttt ccagttcacg aatatttccc
     3601 ggccagtcgt agtggatcag caaatccatc gcctgtggcg taaagccttt taccgccttg
     3661 cgattacgct cggcaaagcg ctgaagaaaa tggccagcca gcagagggat atcttcccgc
     3721 cgttgccgca gcgatggtac ttcaatcgcc accacattca ggcgatagta gagatcctgg
     3781 cgaaaacgcc cggcattcac ctctgcggca agatcgcgat gggtcgccgc aatcagccgg
     3841 acatcaaccg agatagtctg gttgctaccg acacgctgaa cttcgcgctc ctgaatcgca
     3901 cgcagcagac gcacctgcat catcggcgag atatcgccaa tttcatcgag aaacagcgtg
     3961 ccgccgtccg cctcaacaaa gcgcccttcc cggcgtttat cggctccggt aaacgcccct
     4021 ttttcgtgac cgaacaattc agattccagc aaggattcgt taagtgccgc acagttgagc
     4081 gttaccagcg gtttttcgct acgtgcgcta ctggcgtgaa tcgccctggc gaccagctct
     4141 ttaccggtgc cggaatcgcc gtggatcagc accgtggctt ccgatggcgc gacgagggcg
     4201 atttcactga gcaggtgttg catcgccggg cttttaccga ccataccgaa ctggctggca
     4261 gtcaccgcag gtgtttcagc atcaatactg tgcgtatgcg cgagcgcctt ttccagcgtc
     4321 gcctgtaggt tatcgaaatc cagcggcttg atgagataat ccagcgcccc agttttcagt
     4381 gcctctaccg ccgtctcgac gctggagtac gcagtcataa tcagcaccgg aattgccggg
     4441 tttaacgctt tgatctcttt cagcgtggcg atgccgtcca tctccgccat tcgcacatcg
     4501 caaagcacaa gatcaaaaac ctgttcccgc acctgctcca gcgcctgtcg cccgctgttc
     4561 gccagcgcga cgttatagcc ccagccgcgc agtaaagcct gcaaaatagt gcagtggcta
     4621 atgtcatcat ccaccaccag aatatcgata ttatcgtgcg tcatccttgt gggtccttac
     4681 gcgtaatatt gaccggaagc cagagggtga acgttgagcc ttttccctcc tggcttgcga
     4741 cctgaattgt accaccgtgt tgttcaacaa tattatgcac gaccgccagc cccagtccgg
     4801 tgccttcggc tttagtggtg aagtacggag tgaagatggc atcaagctga tctgccgcaa
     4861 ttcccttacc gctgtcggta acgctgattt tcacgcccgc gccgctttcg ctggccgtca
     4921 cgctaatcac gccatgctga ccaatcgcct gaatagcatt gagatagaga ttcaacagga
     4981 cctgagtcag cctgtccgga tcggcctgaa tttccggtaa tgtgtcgttg gcggtaaagc
     5041 gtaactggat ctcccggctg tttgcatcct ggcttaccag ctgtaatgag tggttaatca
     5101 gcgtgttgag atccaccgcc tgcaaagcca gatgcgttgg cttaaccagt tccagcaact
     5161 cgcttaccac gcggtttaaa cggtcggcct ctttcgccat cacctgcgcc agttgatgcg
     5221 cttctccccc tgcaggcgcg cgctcggcaa agtattttgc cagtccttta atcgaggaaa
     5281 gtgggttacg gatttcgtgg gcaacgcctg ccgcaagatg ccccagcgcc accagcttct
     5341 ctttgcgctt catttcatct tgtagaagct ggcgcgagcg cagatagcgg cgataccaga
     5401 agaatgacaa tacgcttgcc agcaagaccg tcgccagggc gaagaggata atcagggtgt
     5461 ttctctgctc acgatcttcg gcactcacaa tattactggc gtcgaaagcg atgaaaattg
     5521 cttgtggtgt cgcggcatac tgctgcatat ggcgcatccg gtgcattcca gcagcaaaca
     5581 tcggttgaaa ctggcgataa atttccagaa caggctcacc gtctgcgcta tcgatccgcc
     5641 gccacgcttc ttcatctccc ggatgtaact gctgcatttc ctgcggggaa taaagctgtt
     5701 ttcccaccat gccggagttg ctatgcatca cgattgttcc ttgttcatcc gtgaccgcaa
     5761 accaacgtac tccaggctgc ccggccattt cttccagtaa ggcctgctgc tgcgcatgat
     5821 gcatgcgcat ccccatgccg acgcgcgagc cggattcgag agcacgaata agtacactgc
     5881 ctttttccag cagcgtttgt ctggcagcgg cagtctcgcg cccataatcg cgaatcaccg
     5941 tcaccgcaaa caaccctacc agcccaacaa tgaccacggg gaggatcgcg cttaaccatt
     6001 tagctaagga gtctttagaa cgttgcataa aacgcatctt cttctttgcc tgctcatccc
     6061 tttttgacag cagaaatcat gccatctttt atcagcgctt accctgcgct gtaacacaaa
     6121 ggcttaagtt tcaatgagta aaaatgactc gctacccgca gcaggcgagt catttttact
     6181 cgtttatcat gccagattac ccgtcatatc agcgtttcat cgttggcacg gaagatgcaa
     6241 tacccgaagt aagacaacca ctggaggatt aaccatgaaa cggaacacga aaattgccct
     6301 ggtgatgatg gcgctttcag caatggcgat gggatcgaca tctgcatttg ctcacggcgg
     6361 acacggtatg tggcagcaaa atgccgcgcc tttgaccagc gaacaacaga cagcgtggca
     6421 gaaaatccat aatgactttt acgctcaaag cagcgcactg caacagcaac tagtgacgaa
     6481 gcgttatgaa tacaatgccc tgttagccgc gaacccaccg gatagcagca aaattaacgc
     6541 ggtcgccaaa gagatggaga atttgcgtca gtcgttagat gagttacggg tgaaacggga
     6601 tattgcgatg gctgaagcgg gtattccgcg cggagccgga atgggcatgg gctacggcgg
     6661 ctgcggtggt ggcggtcata tgggtatggg ccactggtaa atcagaacgt tttctcgttg
     6721 ggttgccagc gacagaaatc agagtttgcc actaacagta attgcgaacc ttcgggcgct
     6781 tccagccatg ccacgctgac atcaacagaa gagcggctct ggcggcgctc gatatgcgct
     6841 atcgcctgcg aatctaaatc aggtttaatg gtttgccctt cgcaggtggt cacggtctgt
     6901 tccatcgcat gccagcgccc ctggcgcagc acgactctcc cctgacgtaa ggcatcgcta
     6961 atctggcgga tctgatcagc ccgataacgg tataaatcga tttggtcgtt agaaagctgc
     7021 tgcttttccc catcggattc acgttgcata aagctcagat caccgcgatc gtcaaaacgc
     7081 gcgcgaatgt tcaccggcgg tttgctgtaa acattgatat tgatgagcgt gaggttatcg
     7141 ccctgccagc gatactctcg cgtcgtggta tcgccactgc gccatgggct aaacacggca
     7201 agcagatgca cattatcgcc gctgtctttt cgccagatgc gtaccgcgcc ctggtcttca
     7261 gcaaatccac tggcggtaaa gggaggaagt gacgagttgt gactacaggc agtgagaaac
     7321 agcgcacctg ccagcatcaa tgatcgacgc cagaaagaca aaaggggtga aaccacccct
     7381 tcgttaaaac tgttcactgc cacgcaatct tacttaactg cgtctttcag tgccttgcca
     7441 gaaacaaatg ccggtacgtt agctgcggca attttgattt ctttaccggt ctgcgggttg
     7501 cggccagtac gctcagcgcg gtggttcact ttgaaggtac cgaaaccaac cagttgtaca
     7561 gcatcgcctt ctttcagaga ctcagtaatt gcagccagag tggactccag agcagcttta
     7621 gcctgggttt tggacagttc tgctttctct gcaattacat caatcagttg agtcttgttc
     7681 ataagttatc cttacaatgt gtttatcgct tgctaagcat cgagtgcgac ggaaatgcca
     7741 gaatagcact ctcctgcata cacgcaccga tagccacttt ttttcgcccc ccaaatgtag
     7801 accagacggg gggcgaaagg gaagactcca ggtatgacaa atcaggcgtt aaatcacgtt
     7861 ttctggtctt attgctgaaa aattatacca atattgctct cacccgcctc acgcaggtct
     7921 gccctaaggc cttttatcag ctcgatatcg cgttcttcgc actcagctaa aagtcggaat
     7981 atttcccact gaatgtccca ttcttgctct acagctgggt tttctttgag ctcttcatcg
     8041 ctcatttcgc gaccagcctg agtcatttcc agcatcgcaa cggtcgtgat ggaggtctta
     8101 ctcacttcca ccgcgtgttc gagcgtttca ccactcaaac gcgaatggac cagttcactt
     8161 aacgccacgc aggcatcgat tgccggataa acgccgtaca gatcgaaatc gtcggctgaa
     8221 ggaatcgctt cttcaaattt ctccagttgg ctgtcgaaat ttacttttgc gtctttgacg
     8281 gtcagcgttt cccagatgag atcgagaata cggcggtaaa tttgcccatc gccaaaaccg
     8341 gtttgctggc agaacatggc gtaattgggg tacatgcgtt cgcataagca agccatgaaa
     8401 gtgacatgct gccagctttc caggcgctcc agacgcagat gaattgggtt ttgtaacatg
     8461 ttgagttctc aaatacggaa attacccgca gtttacctga attagggctg atttgctgta
     8521 tagcgcacga acgctggacg ttccgaggcc accgcgtccg cccagcgcgt tggctccggc
     8581 agacgatagc ctttcatgca gcgttgtacc cacgccagcg cgctgtccac gctgacccga
     8641 tggccggtag cgataaacaa cgggttacag cgcgctttgc tgcgccagac ccaggccagc
     8701 tgctcgcctt tatccatcag tggggccagc gcgcccggtt cgctggagag cggttcgaat
     8761 ttaccgcata gtcgtttttt cgctacgcca atggtcggca catccaccag caaaccaaaa
     8821 tggctggcga cgccaagacg gcgaggatgc gagatcccat gaccatcgac aaacactaaa
     8881 tccggttttt gtgacagcat ctcccacgct gccaggagcg caggatattc gcggaaggaa
     8941 agaaaacctg gaatgtaagg catggtggta gcgatgcggg ccactttata ctccaccagc
     9001 tcaagcgagg ggtatttcag cagcaccatc gccgctcgcg tcacttctcc gccctgctca
     9061 aacccgacat cggctccggc gatcagatcc ggcggatcct tatcgagtcg atcctcgcgg
     9121 atcacagaag aagccagttc gatttgttga gcgcgtaatg acgcgagatc cataatcgct
     9181 ccttagcggt gatactgttc agacagtcga tgcactgcct ccacgaacac gccagcatgt
     9241 tctggcggca catcctgatg aatgccgtga ccaaggttaa agacatgacc ttcgccgtga
     9301 ccgaaacctg caagtatagt cgctacttct tcttcaatgc gggcaggcgg cgcgtacagc
     9361 atcgacggat ccatattacc ctgcaacgcg actttattgc ccacacggcg gcgcgcatcg
     9421 gcgatgtccg ttgtccagtc gaggcccagc gcatcgcaac cggtttctgc catggcttcc
     9481 agccactgtc cgccgccttt ggtaaacagc gtgaccggta cgcggcgacc gtcgttttca
     9541 cgcagtaaac catcaacaat tttatgcatg taataaagcg agaactgttg ataatcgcgc
     9601 ccggtaagca cgccgcccca ggtgtcgaaa atcatcacag cctgagcacc ggctttaatc
     9661 tgcgcattca gatacaaagt gacgcttttc gccagtttat cgagtagagc gtgcagcgcc
     9721 tgcggatcgg catacatcat ttttttgatc acggtgaagg ctttgctgct gccgccttcc
     9781 accatgtagg tcgccagcgt ccacgggctg ccggaaaaac caatcagcgg gacgtcgcct
     9841 ttcagttcgc ggcgaatggt acgcaccgcg ttcatcacgt aacccagctc atcttccggg
     9901 tccggaattg gcagtttatc gacgtcagct ttgcaggtga ctggcgaggt aaaacgcgga
     9961 ccttctccgg cctcaaaata gagccctaac cccatcgcgt ccggcacggt gaggatatcg
    10021 gaaaagagga tcgccgcatc cagcgggtag cgacgcagcg gttgcaaagt cacttcgcac
    10081 gccagctcgg cgtttttgca cagcgacata aaatcgcctg cctgggcgcg cgtggcttta
    10141 tattccggta gatagcgacc cgcctggcgc atcatccata ctggagtgac atcaacgggc
    10201 tggcgcagca gcgcccgcag ataacgatcg tttttaagtt cggtcatttt ggctgttcct
    10261 tagtgcgtca ggcggtcagt gtatcatcac tcgtactctg cccgacacat cgccaccgta
    10321 tcttctatca gacggcgcgc cacggtgccg ggcggcggga gtaacggcaa atcgtcatag
    10381 cgataccagt tcgcctcgag caattctttc gggtcgatca cgatgtcgcc gctgtcatat
    10441 tccgccataa acgcggtcat taaagactga ggaaacggcc acggctggga agtcacataa
    10501 cgcaagtttt taactttaat tccgctctct tccatcactt cccgcgcgac cgcctgctcg
    10561 agggtttcgc ccacttcgac gaatccggca agtaccgtat ggacaccgtt acgatggcgg
    10621 gtatgctggg cgaggaggat cgaatcatcg cgacggatgg caacaataat gcagggggcg
    10681 atttgcgggt agtaacgctc acggcaatgg ctgcacagca tcgcccattc ggttttgctt
    10741 ggatacattt catgcccgca gtaaccacag tatttatgcg atcggtaaaa ctccgccagt
    10801 tgcacgccac gtccggccag ttgaaacagc ccaacatcga gatcaatgac ctgacgtacc
    10861 gaccccatat cgtgacgccg ctgctgttgt attaaccaaa caggttcccc ctgccattcg
    10921 ccgatttgta gtgcgcgctg acccacaaga tcgaaatttg ccgcttcgcc atatggcaat
    10981 tctcccttcg gcaaccataa tttttgttca tggctgacga cccagcagcc gtgatctaat
    11041 ttttcaatta tacgatccat agctcttgca ctacctttgc atcactggca tgtttaacat
    11101 ggtttttaca tttctcactg agcagttttt gaatacaaac ttgcggagtc aatcatgctt
    11161 aaccagctcg ataacctgac ggagcgcgtc agaggaagta acaaactggt tgatcgctgg
    11221 ctacatgtac gtaagcatct gctcgtggct tactacaatc tggttggcat taagcctggc
    11281 aaagaatcgt acatgaggct aaacgaaaaa gcccttgatg atttttgtca gagcctggtc
    11341 gattacttgt ctgccggaca tttcagtatt tatgagcgca ttcttcataa gctggaaggc
    11401 aacgggcaac tcgcgcgcgc cgcgaagatt tggccgcaac tcgaagccaa tacccaacag
    11461 attatggatt actacgattc cagcctggaa accgctatcg atcatgataa ttaccttgag
    11521 tttcaacagg ttttatctga catcggcgag gcgctggaag cgcgctttgt gctggaagat
    11581 aagctgattc tgctggtgct tgacgccgcc cgcgtcaaac atcctgcttg agttctgcgc
    11641 tgttaacgcg taatttacat tcaacgcccc gtttgtaggg ctaatttctt gtcggagtgc
    11701 cttaactggc tgagaccgtt tattcgggat ccgcggaacc tgatcaggct aatacctgcg
    11761 aagggaacaa gagttaatct gctatcgcat cgcctttgcg gcgatcgtct cttgcttcat
    11821 ccgtcgtctg acaagccacg tccttaactt tttggaatga gctatgtctg caacaaaact
    11881 gacccgccgc gaacaacgcg cccaggccca acattttatc gacaccctgg aaggcaccgc
    11941 ctttcccaac tcaaaacgca tttacatcac tggcgcacac cccggcgtgc gcgtgccgat
    12001 gcgtgagatc cagcttagcc cgacgctaat cggcggcagc aaagaacagc cgcagtacga
    12061 agaaaacgaa gcgattccgg tctacgacac ctccggcccg tatggcgatc cgcagatcgc
    12121 cattaacgtg caacaagggc tggcaaaact acgccagccg tggatcgatg cgcgcggcga
    12181 taccgaagaa cttaccgtgc gcagttccga ttacactaaa gcgcggctgg cagatgatgg
    12241 cctcgacgag ctgcgtttta gcggcgtatt aacgccaaaa cgcgccaaag caggacgccg
    12301 cgtcacacaa ctgcactacg cccgccaggg catcatcaca ccggaaatgg aattcatcgc
    12361 catccgcgag aatatgggcc gcgagcgcat ccgtagcgaa gttttacgcc accagcatcc
    12421 gggaatgagc tttggcgcac gtctgccgga aaatatcact gcggaatttg tccgtgatga
    12481 agttgctgcc ggacgtgcga ttatcccggc caacattaat catccggaat cggagccgat
    12541 gattattggc cgtaacttcc tggtaaaagt taacgccaat atcggcaact cagcggtcac
    12601 ctcttccatc gaagaagaag tggaaaagct ggtatggtcc acgcgctggg gagcggatac
    12661 ggtgatggat ctctccaccg gtcgctatat tcacgaaacc cgcgagtgga ttttgcgtaa
    12721 cagcccggtg ccgatcggta cagtgccgat ctaccaggcg ctggagaagg ttaacgggat
    12781 cgccgaagat cttacctggg aagcgttccg cgacacgctg ctggaacagg ccgagcaagg
    12841 tgtggattac ttcactatcc atgcgggggt gctgctgcgc tatgtgccga tgaccgcgaa
    12901 acgcctgacc ggtatcgtct cgcgcggcgg ttcgattatg gcgaaatggt gcctctccca
    12961 tcatcaggaa aatttcctct atcaacactt ccgcgaaatt tgtgaaatct gtgccgctta
    13021 tgacgtttcg ctgtcgctgg gcgacggtct gcgccccggt tctattcagg acgccaacga
    13081 tgaagcacag tttgccgagc tgcatacgct gggcgaactg accaaaattg cctgggaata
    13141 tgacgtgcag gtgatgattg aaggcccagg ccacgtgccg atgcagatga tccgccgcaa
    13201 tatgaccgag gagttagagc actgccacga agcgccgttt tacactctgg ggccgctaac
    13261 taccgatatt gcgccgggct atgaccactt cacgtcgggg attggtgcgg cgatgattgg
    13321 ctggtttggc tgcgcgatgc tctgttacgt aacgccaaaa gagcatctgg gtctgcctaa
    13381 taaagaagat gttaagcagg ggcttatcac ctataagatt gccgcccacg ccgctgacct
    13441 ggcgaaaggg catccgggcg cgcaaattcg cgataacgcc atgtcgaaag cccgcttcga
    13501 atttcgctgg gaagaccagt ttaatctggc cctcgacccg tttaccgccc gcgcttatca
    13561 cgatgaaacc ctgccccaag agtccggcaa agtcgcccat ttttgctcca tgtgtggacc
    13621 gaaattttgc tcgatgaaaa tcagccagga agtgcgtgat tacgccgcca cgcaaactat
    13681 tgaaatggga atggcggata tgtcggagaa cttccgtgcc agaggcggag aaatctacct
    13741 gcgtaaggag gaagcgtgat gtatcagcct gattttcctc ctgtaccttt tcgtttagga
    13801 ctgtacccgg tggtggacag cgtacagtgg atcgaacgtc tgttggatgc aggcgtacgt
    13861 actctccagc tacgcatcaa agatcggcgc gatgaagagg tggaagccga tgtcgtggcg
    13921 gcaattgcgc tgggccgccg ctataacgcg cgattgttta tcaacgatta ctggcggctg
    13981 gcgatcaagc atcaggcgta tggcgtccat ttggggcagg aagatttgca agccaccgat
    14041 ctcaatgcca tccgcgcggc aggcctgcgg ctgggcgttt cgacacatga cgatatggaa
    14101 atcgacgtcg cgctggcagc acgcccctct tatatcgcgc tgggacatgt gttcccgacg
    14161 caaaccaaac agatgccttc tgcaccgcag gggctggaac agctggcacg gcatgttgag
    14221 cgcctggcgg attatcccac cgtggcgatt ggcggtatca gtctggcgcg tgcgcctgcg
    14281 gtgatagcaa cgggtgtcgg cagtatcgcc gtcgtcagcg ccattactca aaccgcagac
    14341 tggcgtttgg caacggcaca gttgctggaa attgcaggag ttggcgatga atgaccgtga
    14401 ttttatgcgt tatagccgcc aaatcctgct cgacgatatc gctcttgacg ggcagcaaaa
    14461 actgctcgac agccaggtgc tgattatcgg tcttggcggg ctgggtacac ctgctgcgct
    14521 atacctggcg ggcgctggcg tcgggacgct ggtactggca gatgacgacg atgtgcattt
    14581 aagcaatctg caacgacaaa tcctctttac cactgaagat atcgatcgcc cgaaatcgca
    14641 ggtcagccat cagcgactga cacagttgaa tcccgatatt caactgacag cattacaaca
    14701 acggttaacg ggtgaggcgt taaaagatgc ggttgcacaa gccgatgtgg tgctcgactg
    14761 taccgacaat atggcgactc gccaggagat taatgccgcc tgcgtggcac tcaacacgcc
    14821 gcttatcacc gccagcgcgg tcggatttgg cggtcagttg atggtactga cgccgcccta
    14881 ggagcagggg tgttaccgct gcctgtggcc agataaccag gagccagaac gcaactgccg
    14941 cacggcgggc gtggttggcc cggtggtcgg ggttatgggc actttgcagg cactggaagc
    15001 cattaagtta ttaagcggta tagagacgcc tgcgggagaa ctccgactgt tcgacggtaa
    15061 gtcgagccag tggcgcagcc tggcgttgcg ccgcgccagt ggttgcccgg tatgcggagg
    15121 aagcaatgca gatcctgttt aacgatcaac cgatgcagtg cgccgccggg caaactgttc
    15181 acgaactact ggagcaactc gaccaacgac aagcgggcgc ggctctggcg attaatcatc
    15241 aaatcgtccc gcgtgagcag tggacgcaac atatcgtgca ggatggcgac cagatcctgc
    15301 tttttcaggt tattgcaggg ggttgagatg ttacgtattg cggacaaaac gtttgattca
    15361 catctgttta ccggcaccgg gaaattcgct tcttcacaac tgatggtgga ggcgatccgc
    15421 gcttccggca gccagctggt gacactggcg atgaaacgtg tcgacttgcg ccagcacaac
    15481 gacgctatcc tcgaaccgct tatcgcggcg ggtgtgaccc tgctgccaaa tacatccggg
    15541 gcgaaaacag cggaagaagc cattttcgcc gcccatctgg ctcgtgaagc gttaggcaca
    15601 aactggttaa aattagagat tcaccctgac gcccgctggc tgttgcccga tcccatcgaa
    15661 accctgaaag ccgccgaaac gctggtacaa cagggatttg tcgtgctgcc ttactgcggg
    15721 gccgatccgg tattgtgtaa acgtctggaa gaagtcggct gtgcagcggt gatgccgctc
    15781 ggcgcgccga ttggctcgaa tcaggggctg gaaacccgcg ccatgctgga gattattatc
    15841 cagcaggcca cagttccggt ggttgtcgat gctggcatcg gcgttcccag ccatgccgcg
    15901 caggcgctgg aaatgggggc cgacgcggtg ttagtgaata cggcgattgc cgtcgcggac
    15961 gatcccgtca acatggcgaa ggcatttcgt ctggcagtag aagcaggcct gctggcacgt
    16021 cagtccggac cgggcagccg cagtcatttt gctcatgcca ccagcccgct gaccggattt
    16081 ctggaggcat cggcatgaaa accttcagca atcgctggcg acaactggac tgggacgaca
    16141 tccgcctgcg tatcaacggc aaaacggctg ctgacgtaga gcgggcgcta aatgcctcgc
    16201 aactcacccg cgacgacatg atggcgctgt tatcgcctgc cgccagtggc tatctggaac
    16261 aactggccca acgggcacag cgtctgaccc gtcagcgatt tggcaacaca gttagtttct
    16321 acgtcccgct ttatctttcc aatctttgcg ctaacgactg cacgtactgc ggattttcca
    16381 tgagtaatcg catcaagcgc aaaacgctgg atgaagcgga tattgccagg gaaagcgccg
    16441 ctatacggga gatgggcttt gaacatctgc tattagtcac tggtgaacat caggcgaaag
    16501 tggggatgga ttactttcgt cgtcatctcc ctgccctgcg tgaacagttc tcttcactac
    16561 agatggaagt gcaaccgctg gcggagacgg aatacgccga gttaaagcaa cttggtctgg
    16621 atggggtgat ggtttatcag gagacatatc acgaggcgac atatgcccgc catcatctga
    16681 aaggcaaaaa acaggacttc ttctggcggc tggaaacgcc ggatcggctg ggccgtgcgg
    16741 ggattgataa gataggcctc ggcgcgctaa ttggcctttc cgacagctgg cgcgttgact
    16801 gctatatggt tgcagaacat ttgctatggc tgcaacagca ttactggcaa agccgttact
    16861 ctgtctcctt tccgcgcctg cgcccgtgta ctggcggcat tgagcctgcg tcgattatgg
    16921 atgaacgcca gttagtgcaa accatctgcg ccttccgact gcttgcaccg gagattgaac
    16981 tgtcactctc cacgcgggaa tcaccgtggt ttcgcgatcg cgttattccg ctggcgatca
    17041 ataacgtcag cgccttctcg aaaacgcagc caggtggcta tgctgataat caccccgagt
    17101 tggaacagtt ctcaccgcac gacgatcgca gaccggaagc ggttgctgca gcgttaaccg
    17161 ctcagggttt gcagccggta tggaaagact gggacagcta tctgggacgc gcctcgcaaa
    17221 gactatgaca cggaatgtaa gaacgtaaat ttattccgag ccgccacgga agatgaaaaa
    17281 cgcaaggttg ttgaaagcgt tgcgtttttt tatggtagtg ccgtcagcag gaatgttgtc
    17341 cagggcgaaa agcttcttcc tcgtttcgcc ctgcctttct taaaaattcc gtaatgcaaa
    17401 gtctgaagcc atcgcctgtt gagcaatgaa agagatctga ttgtaagaga gtaaatactc
    17461 aactatgata gagacgaaaa taagaacaca tgttctcatc ttccaggatg cagcagactg
    17521 aagaaattca gacatcccgc aacctgcgat tatcgcaagg tcaaggcaaa gtccggtaat
    17581 ggcgttctga ataccagaga taattctctg gcgaaaccca ccttaaggtg ggttttgtta
    17641 ttttgagggc tgaggaagct gcttatttct caataagttg taggcaatgg tgaaaaattt
    17701 cactgattta cctttctgtt tttcagaaat gggataagcg ctagttacat gcaaacgaag
    17761 ttttttcttt tccctgaaag ccctgaacgc aacaaagtaa aatgccttgt ctcctccgtc
    17821 taagtccacc tcaatcacgg catagctacc atacccggca tgaataacgt tgctttctgg
    17881 cagcgccaaa atagttcttt ttaagtgtgt gcgcgctaag ttataacggt gctggcagaa
    17941 gggacgagat tctttaggcg cgtggtacat taacgattgt ttttcttcgt tcgtctgatg
    18001 ttcataatct tttgtgaagc agtgaagaga gtaggtaacc caaaagcgat actctaaagg
    18061 ctctttgccc tcatgatgct gaacgtagac agcctgatga gcatcaagaa atgataaatc
    18121 acgatatcac catccggatg tccaaaaggt cgccactttt ccacttcctg tttcattctc
    18181 gttccttaag aaagttacac aggaatagta tcgaatcctt gccgagtttg actcaattag
    18241 ttcagtcagt ttcaggatat tagtcatctc tacattgatt atgagtatcc agaaattcat
    18301 taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc cgccgaagcg
    18361 ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc agacctgcgt
    18421 tcagcagttc tgccaggctg gcagatgcgt cttctgcagt cacctgcggt gcagccggag
    18481 cttcacccgc agcacggcga cgcatacgat cctggtggta cgcgtaaccg gtacctgccg
    18541 ggatcagacg acccacgata acgttctctt tcaggccgcg cagttcgtcg cgtttgcccg
    18601 caacggctgc ttcggtcagc acgcgagtgg tctcctggaa cgatgccgcg gagatgaagg
    18661 actcggttgc cagagacgct ttggtgatac ccagcagatc gcgggagtaa gttgcaccca
    18721 ctttgccgtt cgcttccagt tcgcggtttg cgatcttgac gcgagagtat tcaacctgtt
    18781 cgccttccag gaagtcggag ctgcccgcat taacgatggt agctttacgc agcatctgac
    18841 gaacgataac ttcgatgtgt ttatcgttaa tcttaacgcc ctgcagacgg tatacgtcct
    18901 gtacttcgtt aacgatgtaa cgagtcacag catgaacacc acgcagacgc agaatgtcgt
    18961 gcggcgcttc cggaccgtcg gaaattacgt caccacgttc tacacgttca ccttcgaaca
    19021 cgttgagctg acgccatttc ggaatcatct cttcgtacgg atcgctaccg tctaccgggg
    19081 tgataaccag acgacgttta cctttggttt ctttaccgaa ggaaacgata ccgctgattt
    19141 cagccaggat tgccggctct ttcggacgac gtgcttcgaa caggtccgca acgcgtggca
    19201 gaccaccggt gatgtccttg gtaccgccgg attcctgcgg aatacgcgcc agggtgtcac
    19261 cagagctgat ctgtacgcca tcttccagct gaacaatcgc tttacccggc aggaagtact
    19321 gcgcaggcat atcggtacct gggatcagaa cgtcgttacc ctgagcatca acgattttca
    19381 gtgccggacg cagatcttta ccacctgcgg tacgttctgc ggaatccaga accaccagcg
    19441 aagacagacc ggtcagttcg tcggtctgac gagtaatggt ctggccgtcg atcatgtcag
    19501 taaagcgtac aaaaccgctt acttcggtga taaccggcat ggtgtgcggg tcccagtttg
    19561 caacggtttc gccgccagca acctgttcgc catcgccttt cgccagtacc gcaccgtaag
    19621 gtactttgta gctttcttta gtacgaccga attcgtcgat cagtttcagt tcggtgttac
    19681 gggaagtgat aaccagttta ccgctggagt tcacaaccga cttcacgttg ctgagcttga
    19741 tgctaccttt gtttttcacc tggatgctgg attcagcagc cgcacgagat gccgcaccac
    19801 cgatgtggaa cgtacgcatg gtcagctgtg tacccggttc accgatggac tgtgccgcga
    19861 taacaccgat tgcttcaccc ttgttgatga tgtggccacg cgccaggtca cgaccgtagc
    19921 agtgcgcaca tacaccaaag tcggtgtcac aagatacaac agaacgtact ttaaccgcgt
    19981 cgacagagtt ctcttccagc aggtcacacc actgttcgtg cagcagcgtg ttgcgcggaa
    20041 cgaggatatc agcagtaccc ggcttcagaa cgtcttcagc agttacacga cccagtacgc
    20101 gatcgcgcag cggctcttta acgtcaccac cctcgataac cggagtcatc atgatacctt
    20161 catgggtacc acaatcgtct tcggtaacca ccaggtcctg cgccacgtca accagacgac
    20221 gagtcaggta accggagttc gcagttttca gtgcggtatc cgccagacct ttacgagcac
    20281 cgtgggtgga gatgaagtac tggagtacgt tcagaccttc acggaagttc gcggtgattg
    20341 gcgtttcgat gatggagcca tccggcttcg ccatcagacc acgcatacca gcaagctgac
    20401 gaatctgtgc cgcagaacca cgcgcaccgg agtcggccat catgtagatg ctgttgaagg
    20461 aaacctgctt ctcttcctga ccgtcacggt taataacggt ttcagtttgc aggttatcca
    20521 tcatcgcttt ggatacacga tcgttcgccg cagcccagat atcgataact ttgttgtagc
    20581 gttcgcccgc agttaccaga ccagactgga actgctcctg aatttcagca acttctgctt
    20641 ctgcctcgga gatgatttcg tgtttcttct ccgggatgac catgtcatcg ataccaacag
    20701 atgcaccaga acgcgctgca tatgcaaagc cggtgtacat gatctggtcc gcaaaaataa
    20761 cggtcggttt cagaccgaga atgcggtagc aggtgttcag cattttggag attgcttttt
    20821 tacccagcgc ctggttgacg atggagtaag gcagaccttt cggtacaatc atccacagaa
    20881 tggcacggcc aacagtcgtg tctttcaggc tggttttcgc tactaattca ccgttagcat
    20941 ctttttcata ctcggtgata cgcactttaa cgcgcgcatg cagagaagcc agaccagagc
    21001 gatacagacg ttctgcttct ttcgggccag tcagcaccat gccttcgcct ttggcgttaa
    21061 cacagtcacg ggtcatgtag tacagaccca gtacaacgtc ctgagacgga acgatgattg
    21121 gttcgccgtt cgccggggac aggatgttgt tggtagacat catcagcgca cgcgcttcca
    21181 gctgggcttc cagcgtcagc ggtacgtgaa cagccatctg gtcaccatcg aagtcggcgt
    21241 tatatgccgc acaaaccagc gggtgcagct ggatagcttt accttcgatc agtaccggtt
    21301 caaatgcctg gatacccaga cggtgcagag tcggtgcacg gttcagcagt accgggtgtt
    21361 cgcggataac ttcgtccagg atatcccaaa cgacagcttc ttcgcgctca accattttct
    21421 tcgcagcttt aatggtggta gcaagaccac gcagttcaag cttgccgtag atgaacggtt
    21481 tgaacagctc cagcgccatt ttcttcggca gaccgcactg atgcagacgc aggtatggac
    21541 ctacggtgat tacagaacga ccggagtagt caacacgctt accgagcagg ttctgacgga
    21601 aacgaccctg tttacctttg atcatgtcgg ccaaagattt cagaggacgc ttgttagaac
    21661 cggtgatcgc acgaccgcga cgaccgttat ccagcagggc gtctaccgct tcctgcagca
    21721 tacgtttttc gttacgtacg atgatgtccg gcgcagccag atccagcaga cgtttcagac
    21781 ggttgttacg gttaatgacg cgacgataca gatcgttcag gtcagaagtc gcgaaacgac
    21841 caccatccag cggaaccagc ggacgcagat ctggcggcag taccggcaga acggtcagga
    21901 tcatccactc tggtttgtta ccagactgaa cgaacgcttc cagcagtttg atacgcttgg
    21961 tcagcttttt acgcttagtt tcggagttgg tttcgttcag ctcttcacgc agctgttcgc
    22021 actcttgctc cagatccatg ctcttcagca gagcctggat tgcttccgca cccattttcg
    22081 cgtcgaattc gtcaccgaac tcttccagcg cgtccagata ctgctcttca gtcaggatct
    22141 gctgacgttc caggttggtc ataccgcctt cgataaccac ataggattcg aagtacagta
    22201 cgcgttcaat atcgcgcagc ggcatatcga gcagcaggcc gatacgggac ggcagcgatt
    22261 tcaggaacca gatgtgcgca gtcggggaag ccagttcgat gtggcccata cgctcacggc
    22321 gtactttagt ctgggtcact tcaacgccac acttctcaca gatgacgcca cggtgtttca
    22381 ggcgcttgta cttaccgcac aggcactcgt aatcttttac cggcccaaag atacgggcgc
    22441 agaaaaggcc gtcacgttct ggtttgaacg tacggtagtt gatggtttcc ggctttttaa
    22501 cttcaccgaa agaccatgaa cggatcatgt ctggcgaagc cagagcaatt ttgatcgcat
    22561 caaactcttc ggttttagtc tgcgctttca gaaactttaa taaatctttc acggatttgc
    22621 tcccgtcgga gttagcacaa tccgctgccg ggttttaccc cgacagcagt gacctgtttg
    22681 agcgagaatt actcgtcttc cagttcgatg ttgataccca gcgaacgaat ctctttcaac
    22741 aatacgttga aggattctgg catgcccggc tccatctgat ggttgccgtc cacgatgttt
    22801 ttatacatct tagtacgacc attcacgtca tcagacttaa cggtgagcat ttcctgcagg
    22861 gtgtatgctg cgccgtatgc ttccagcgcc cacacttcca tctccccgaa acgctgacca
    22921 ccgaactgtg ccttaccacc cagcggctgc tgagtaacca ggctgtaaga accggtggaa
    22981 cgcgcgtgca tcttgtcgtc gaccaggtgg ttcagtttca gcatgtacat gtaaccaacg
    23041 gttaccggac gctcgaactg ttcaccagtg cgaccatcgt acaggcggat ctgaccggaa
    23101 gtcggcaggt cgccaagttt cagcagctct ttaatttctg cttctttcgc accgtcgaac
    23161 accggcgttg cgattggcat acctttgcgc aggttttcag ccagacgcat aacttcttca
    23221 tcgctgaagg tactcaggtc aactttctga cgaacgtcag cgcccagatc gtacgcacgc
    23281 tggatgaatt cgcgcagttt cgcgacttct tgctgctgtt tcagcatggc gttgatcttg
    23341 tcgccgatac ctttcgcagc catacccagg tgggtttcga ggatctgacc gatgttcata
    23401 cgagacggta cgcccagcgg gttcagtacg atgtctaccg gcgtaccgtt ttcatcgtaa
    23461 ggcatatctt cgatcgggtt gatcttagaa attacaccct tgttaccgtg acgacctgcc
    23521 atcttgtcac caggctggat acggcgttta accgccagat ataccttaac aatcttcagc
    23581 acgcccggtg ccagatcgtc gccctgggtg attttgcggc gtttcgcttc gagtttcttc
    23641 tcgaactcgt gtttcagttc gtcatactgc tcagccagct gttccagctg attttgtttc
    23701 tcttcgtcgg tcaggcccag ctccagccag cgatcgcgcg gcagtttgtc gagcttctca
    23761 gcttcaacgc caccggctac cagcacagca cggatacggc tgaacagacc cgcttcgagg
    23821 atctgcagtt cttcagacag gtctttcttc gcctgtttga gctgcatttc ttcgatttcc
    23881 agcgcacgtt tgtctttttc tacgccatcg cgagtaaaga cctgaacgtc gataaccgta
    23941 ccggatacac cgtttggtac gcgcagagaa gagtctttaa cgtcagacgc tttctcaccg
    24001 aagatcgcac gcagcagttt ttcttctggg gtcagctgag tttcaccttt cggcgtaacc
    24061 ttaccaacca gaatgtcgcc accggtcact tccgcaccaa tataaacgat accggattca
    24121 tccagtttgg agagcgcagc ttcacccacg ttcgggatgt cagcggtgat ctcttccggc
    24181 cccagcttgg tgtcacggga cacacacgcc agttcctgaa tgtggatggt ggtgaaacgg
    24241 tcttcctgaa caacacgctc ggatacgagg atggagtctt cgaagttgta accattccac
    24301 ggcatgaacg ctacgcgcat gttctgacca agcgccagtt caccgaggtc ggtggacgga
    24361 ccgtctgcca gcacgtcgcc acgttcaacc ggttcaccca gagacacaca cggcatctgg
    24421 ttgatacagg tgttctggtt agaacgggtg tatttggtca ggttgtagat gtcgatacct
    24481 gcttcacccg gatacatctc gtcttcgtta actttgataa cgatacggga agcatccacg
    24541 tactgaacga caccaccacg tttagcaacc gcagttacac cggagtcaac ggcaacagca
    24601 cgttccatac cagtaccaac cagcggctta tcagcacgca gagtcggaac ggcctgacgt
    24661 tgcatgttcg cacccatcaa tgcacggttg gcgtcatcgt gttccaggaa cgggatcagg
    24721 gacgcaccga cggataccac ctgctgggtg gatacgtcca tgtagtcaac ctggtcacgg
    24781 ctgaacaagc tggattcgcc tttgctacgg caggttacca ggtcttctac gaagtggcct
    24841 tcttcatcca ggttggagtt cgcctgggcg ataacgtagt tgccttcttc gatagcagac
    24901 aggtagtgaa tttcgtcagt tacaacaccg tcggtcactt tacgatacgg agtctcaagg
    24961 aagccgtatt cgttagtctg tgcgtacacg gacagagagt tgatcagacc gatgttcgga
    25021 ccttcagggg tttcgattgg acatacgcga ccgtagtgag tcgggtgtac gtctcgaact
    25081 tcgaagcctg cacgttcacg ggtcagaccg cctgggccga gtgcggagat acgacgtttg
    25141 tgcgtaatct cagacagcgg gttgttctgg tccataaact gagacagctg gctggaaccg
    25201 aagaactctt tcactgctgc ggaaatcggc ttggcgttga tcatatcctg aggcatcagg
    25261 gtatccagat cgcccagaga cagacgctct ttcaccgcac gctctacacg taccaggcca
    25321 acgcggaact ggttttccgc catttcgcca acggaacgga tacgacggtt gccgaggtgg
    25381 tcgatatcat cgacttcgcc tttaccgtta cggatatcga tgagcttttt cataacatca
    25441 atgatgtcgt ctttgctcag gataccagaa ccttcgattt cttcgcgcag cagagaacgg
    25501 ttgaacttca tacgaccaac cgcagacagg tcataacggt cttcggagaa gaacaggttc
    25561 tcgaacaggc tttccgctgc ttcacgagtc ggcggctcgc cagggcgcat catgcggtag
    25621 atttctacca gtgcgctcag acggtcgtta gttgggtcga cacgtaaggt ttcagagata
    25681 tatgggccgt gatccagatc attggtgaac agcgtttcga tacgcttgtg accagactgg
    25741 ctcagcttag ccagcagatc caggctcagc tccatgttcg ctgcgcagat cagctcgccg
    25801 gtagactcat caatatagtc tttagcaacc actttacctg cgatgtactc aaccgggact
    25861 tcgatcagtt tgacgtcgtc tttttccagc tggcgaatgt ggcgcgcagt gatacggcgg
    25921 cctttttcta cgtacacttt accgttagct tcgatgtcga aagatgcggt ttcaccacgc
    25981 aggcgttccg gcaccagttc catctgcagc ttgttatcac ggatttcaaa gataactttt
    26041 tcaaagaaca ggtcgaggat ctgctctgtg gtgtagttca gggcacgcag aatgatggtc
    26101 gcaggcagtt tacggcgacg gtcgatacgt acgaacaggt tgtccttcgg atcgaattcg
    26161 aagtccagcc aggaaccacg gtaagggatg atgcgcgcgt tatacagcac tttacccgaa
    26221 gagtgggttt tacctttgtc ggagtcaaag aagacgcccg gactacggtg cagctgggaa
    26281 acgataacac gctcagtacc gttgataaca aaggtaccgt tgtctgtcat gagcggaatt
    26341 tcgcccatgt agacttcttg ttctttaatg tcttttacgg tgccttccgg cgcttcgcgc
    26401 tcatagatca ccagacgcag tttaacgcgc agcggtgcgg aataggtcac gccacggatt
    26461 tgacattcct ggacgtcaaa caccggttcg ccaaggcggt agctgacgta ttgcagctcg
    26521 gaattaccgc tgtagctctg aatcgggaat acggaacgga aagcagcttc cagaccatac
    26581 tgcccttcag gatcttgctc gataaatttc tgaaacgagt caagctggat agaaaggaga
    26641 taaggtacat ccagaacttg tggacgttta ccaaaatcct tacgaatacg ttttttctcg
    26701 gtataggagt aaaccatagg gttcctcagc tcgctgacaa gtcgacccat ctgtccattg
    26761 agcggacagt ttgtgcaaca ctattttgtt gaccggaaaa tggaacactt tccgcaatgc
    26821 ctgttgctat cacgcttaaa ccatttcatt gcgatttaca cagaacggac gtcctgtcgc
    26881 agtatattaa gtcgtcgata gaaacaagca ttgaaaggca cagcagtagt caaacagtgt
    26941 gaaacgctac tggcgcctta cagcgcaaaa aggctggtga ctaaaaagtc accagccatc
    27001 agcctgattt ctcaggctgc aaccggaagg gttggcttat ttaacttcaa cttcagcgcc
    27061 agcttcttcc agagcttttt tcagtgcttc tgcgtcgtct ttgctcacgc cttctttcag
    27121 agcagccggt gcagattcta ccaggtcttt agcttctttc agacccaggc cagttgcgcc
    27181 acgtactgct ttgataacag caactttgtt agcgccagca gctttcagaa ttacgtcgaa
    27241 ttcagttttt tcttcagcag cttcaaccgg gccagcagct acagctacag cagcagcagc
    27301 ggaaacaccg aatttttctt ccattgcaga gatcagttct acaacgtcca ttacagacat
    27361 agctgcaact gcttcaatga tttgatcttt agtgatagac atttaaattg ttcctgaata
    27421 tcagaataag tttatacgta agcgaatgcg ttaaaaagat aactgcgatt aagcagcttc
    27481 tttcgcatcg cgtacagcag ccagagtacg aaccagtttg ccagccgaag cttctttcat
    27541 ggttgccatc aggcgtgcaa ttgcttcttc gtaggtcggc agagttgcca ggcggtcgat
    27601 ctgagacgcc gggatcagct caccttcaaa ggcagcggct ttgacctcaa attttgcatt
    27661 cgctttcgcg aactctttga acagacgagc agcagcgccc gggtgttcca tagagtatgc
    27721 aatcagggtc ggaccaacaa acgcgtcttt caggcactcg aacggagtac cttcaacagc
    27781 gcggcgcagc agggtgttac gaacaacacg catgtatacg ccagcttcgc gacctgcttt
    27841 acgcagttca gtcattttat ctacagttac gccacgggaa tccgcaacta ctgcagacag
    27901 cgcgcctttg gctacttcgc tgacttcagc aacaatcgct tgtttgtctt gaagatttaa
    27961 agccattagc tttgctcctg gatgtttgcc agagaaaatc tctggaactc acttcactcc
    28021 tccaaacgga gagagcgtct taattacggt gagcagaaac aagccagaat ataaaagaat
    28081 aatcttagcg ttctgtcacc gtctacgcag gggattaagt ttcttgtgaa actcctgcgg
    28141 tcttcgacgg aggcctggat aggccaggct ccaacgaaca aatcttttta gctgctaact
    28201 ttcgttagca tacgtggggg taagattgta gacaaaatca ccgcccacgt aaaggcatta
    28261 gtttacagaa gcgctcaggc cagcctggtc aactgcaaca cctgcaccca tggtggtgga
    28321 gatgctaact ttcttgatgt acacgccttt cgcctgagtc ggttttgctt ttttcagcgc
    28381 aaccagcaga gcttccaggt tttctttcag tttgtcagcg tcaaagtcca ctttaccgat
    28441 ggtggtgtgg atgatgccgt ttttgtcgtt acggtaacga acctggccag ctttagcgtt
    28501 tttaaccgct tcagcaacgt tcggcgttac agtacccact ttcgggtttg gcatcaggcc
    28561 gcgcggaccc agaacctggc ccagctggcc aacaacgcgc attgcatccg gagaagcaat
    28621 aacaacgtca aagttcattt cgcctttctt gatctggtca gccagatctt ccatacctac
    28681 cagttcagca cctgcagctt tagcagcttc agcgtttgca ccctgggtaa atacggctac
    28741 gcgaacggaa cggccagtac cgtgcggcag tacagttgca ccacgtacgt tctggtcaga
    28801 tttacgagcg tcgatgccga ggttaacagc aacgtccacg ctttctacga atttagcagt
    28861 cgccagctct ttcagcagag cgatagcttc gttgatgtcg tactgtttag ttgcatcaac
    28921 tttctcgcgg ataacacgca tgcgcttggt cagtttagcc atttcttagt cctccactac
    28981 caggcccatg gaacgtgcag taccttcgat ggagcgagtc atcgcttcaa tgtcggcacc
    29041 agtcatgtcg gcagctttgg tctgcgcgat ttcctgcagc tgagcgcggg aaattttacc
    29101 cactttgtct ttgttcggct taccggaacc agacttgata ccagccgctt ttttcagcag
    29161 aactgctgcc ggcggagtct tggtaacgaa agtgaaagaa cggtcagcgt aaacggtgat
    29221 tactaccgga atcggcagac ctttttcgat ggaatcagtt tttgcgttga acgctttgca
    29281 gaattccatg atgtttacgc cctgctgacc cagagccgga cctactggcg gactcgggtt
    29341 agccatacca gctgcaacct gcagcttgac ataggcttgt actttcttag ccattataaa
    29401 ttcctcaagt tgggtaataa cgcctctgag aggctccccg tgatataaaa aatcgtttta
    29461 cgggcaaggc ccataaaaac aaaaggcgcg aaattgtatt ccaatctcgc gccttgtgca
    29521 acgattaaat cgccgctttt ttgatcgctg ggttaggctt tttcaacctg gctgaagtcc
    29581 agctctaccg gggtcgcacg accgaagata gaaacagaca ctttcagacg agatttctcg
    29641 taatccactt cttcaacaac accgttgaag tcagcgaacg gaccatcatt aacacggacc
    29701 atttcacccg gttcaaacag cgttttcgga cgcggcttat caccaacctg ctgcaggcgg
    29761 ttcataatcg catcgacttc tttatcgctg attggcgcag gacgatcgga agtaccgccg
    29821 atgaagccca tcacacgcgg tacgctgcgc accaggtgcc agctcgcgtc gttcatcacc
    29881 atctgaacga ggacgtagcc agggaagaat ttacgttcgc ttttgcgacg ctgaccgcca
    29941 cggatttcaa ccacttcttc ggttggtacc atgacttcac caaacaaatc ttccatgttg
    30001 tgtaatttga tatgctcacg cagcgacgtt gctacgcggc cttcaaaacc ggaaaacgcc
    30061 tgaacgacgt accagcgctt tttaggagct tcagacatct cagaacctca ggccagtgat
    30121 aaaggatacc aggcgaacca gaataccatc cagtccccac aggatcagtg acattactgc
    30181 ggtaaccgca gccacaatca gcgtggtgtg caatgtttcc tggcgagtcg gccaaatgac
    30241 cttacggact tcggtacgcg cttcacgggc aaaagcaacg gtagctttac cttttgtcgt
    30301 taacagcgcg acaccacccg ctgcagcaat cagaattact acggccagcg cacgcagcgg
    30361 cagcataatg tcgcgataaa ggtagttgcc gacaatcgcc accaggagca atgccaccac
    30421 aacgacccac ttcatcgctt ccaggccgcg cccgcttcct tgagcttcgg tattcgcact
    30481 cataaaccaa cctgtcagaa gtattctaca aacattttca ccccgcgatc gcgaggcaaa
    30541 ccaaatcgaa acgcgtatta gcttttcgga ttatacgccc tcaacagagc ctgtctcagc
    30601 aatgattatg acaaagaaaa tcactgatga gccaggttct ggtacgaaag cgtgcaaaaa
    30661 gggcatcaaa tgatgccctt ttagtgcgca ttgcgtcaaa tgttatcggc aattagctca
    30721 gaacttt
//
LOCUS       DAZGCQ010000056        30506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000056 DAZGCQ010000000
VERSION     DAZGCQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.056"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(36..111)
                     /locus_tag="ACHK07_002783"
     tRNA            complement(36..111)
                     /locus_tag="ACHK07_002783"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Gly,seq:gcc)
     gene            complement(322..867)
                     /gene="orn"
                     /locus_tag="ACHK07_002784"
     CDS             complement(322..867)
                     /gene="orn"
                     /locus_tag="ACHK07_002784"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8103095.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            962..2014
                     /gene="rsgA"
                     /locus_tag="ACHK07_002785"
     CDS             962..2014
                     /gene="rsgA"
                     /locus_tag="ACHK07_002785"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8103096.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2111..3079
                     /gene="asd"
                     /locus_tag="ACHK07_002786"
     CDS             2111..3079
                     /gene="asd"
                     /locus_tag="ACHK07_002786"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8103097.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3101..6424
                     /gene="mscM"
                     /locus_tag="ACHK07_002787"
     CDS             3101..6424
                     /gene="mscM"
                     /locus_tag="ACHK07_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8103098.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6575..8077)
                     /gene="yjeM"
                     /locus_tag="ACHK07_002788"
     CDS             complement(6575..8077)
                     /gene="yjeM"
                     /locus_tag="ACHK07_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8103099.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8296..9273)
                     /gene="epmA"
                     /locus_tag="ACHK07_002789"
     CDS             complement(8296..9273)
                     /gene="epmA"
                     /locus_tag="ACHK07_002789"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8103100.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9598..11406
                     /gene="frdA"
                     /locus_tag="ACHK07_002790"
     CDS             9598..11406
                     /gene="frdA"
                     /locus_tag="ACHK07_002790"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8103101.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11399..12133
                     /gene="frdB"
                     /locus_tag="ACHK07_002791"
     CDS             11399..12133
                     /gene="frdB"
                     /locus_tag="ACHK07_002791"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8103102.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12144..12539
                     /gene="frdC"
                     /locus_tag="ACHK07_002792"
     CDS             12144..12539
                     /gene="frdC"
                     /locus_tag="ACHK07_002792"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8103103.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12550..12909
                     /gene="frdD"
                     /locus_tag="ACHK07_002793"
     CDS             12550..12909
                     /gene="frdD"
                     /locus_tag="ACHK07_002793"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8103104.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12972..14105
                     /locus_tag="ACHK07_002794"
     CDS             12972..14105
                     /locus_tag="ACHK07_002794"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8103105.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14194..14727
                     /gene="blc"
                     /locus_tag="ACHK07_002795"
     CDS             14194..14727
                     /gene="blc"
                     /locus_tag="ACHK07_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8103106.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14724..15041)
                     /gene="sugE"
                     /locus_tag="ACHK07_002796"
     CDS             complement(14724..15041)
                     /gene="sugE"
                     /locus_tag="ACHK07_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8103107.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15217..15363)
                     /gene="ecnB"
                     /locus_tag="ACHK07_002797"
     CDS             complement(15217..15363)
                     /gene="ecnB"
                     /locus_tag="ACHK07_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8103108.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15474..15599)
                     /gene="ecnA"
                     /locus_tag="ACHK07_002798"
     CDS             complement(15474..15599)
                     /gene="ecnA"
                     /locus_tag="ACHK07_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8103109.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15651..16217)
                     /gene="efp"
                     /locus_tag="ACHK07_002799"
     CDS             complement(15651..16217)
                     /gene="efp"
                     /locus_tag="ACHK07_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8103110.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16259..17287
                     /gene="epmB"
                     /locus_tag="ACHK07_002800"
     CDS             16259..17287
                     /gene="epmB"
                     /locus_tag="ACHK07_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8103111.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17682..18551
                     /locus_tag="ACHK07_002801"
     CDS             17682..18551
                     /locus_tag="ACHK07_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8103112.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18744..19097)
                     /locus_tag="ACHK07_002802"
     CDS             complement(18744..19097)
                     /locus_tag="ACHK07_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO8103113.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19235..20881)
                     /gene="groL"
                     /locus_tag="ACHK07_002803"
     CDS             complement(19235..20881)
                     /gene="groL"
                     /locus_tag="ACHK07_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO8103114.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20925..21218)
                     /locus_tag="ACHK07_002804"
     CDS             complement(20925..21218)
                     /locus_tag="ACHK07_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO8103115.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21494..22750
                     /gene="yjeH"
                     /locus_tag="ACHK07_002805"
     CDS             21494..22750
                     /gene="yjeH"
                     /locus_tag="ACHK07_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO8103116.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22766..23242)
                     /locus_tag="ACHK07_002806"
     CDS             complement(22766..23242)
                     /locus_tag="ACHK07_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO8103117.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            23579..25015
                     /gene="aspA"
                     /locus_tag="ACHK07_002807"
     CDS             23579..25015
                     /gene="aspA"
                     /locus_tag="ACHK07_002807"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO8103118.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            25133..26434
                     /gene="dcuA"
                     /locus_tag="ACHK07_002808"
     CDS             25133..26434
                     /gene="dcuA"
                     /locus_tag="ACHK07_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO8103119.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26550..26888
                     /gene="cutA"
                     /locus_tag="ACHK07_002809"
     CDS             26550..26888
                     /gene="cutA"
                     /locus_tag="ACHK07_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO8103120.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26864..28561
                     /gene="dsbD"
                     /locus_tag="ACHK07_002810"
     CDS             26864..28561
                     /gene="dsbD"
                     /locus_tag="ACHK07_002810"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO8103121.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28598..29173
                     /locus_tag="ACHK07_002811"
     CDS             28598..29173
                     /locus_tag="ACHK07_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8103122.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            29280..29355
                     /locus_tag="ACHK07_002812"
     tRNA            29280..29355
                     /locus_tag="ACHK07_002812"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29313..29315,aa:Phe,seq:gaa)
     gene            29553..>30506
                     /locus_tag="ACHK07_002813"
     CDS             29553..>30506
                     /locus_tag="ACHK07_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8103123.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTILAEREPLPAVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         7490 a         7974 c         7702 g         7340 t
ORIGIN      
        1 tgggtggtct gtgctttgca gcacttttca aattttggag cgggaaacga gactcgaact
       61 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cgtactactt
      121 aattttgctt tcgtaatttt cgcatttctg cgtcgttacg ggaggcgcat tatacgagaa
      181 atctttccct ctgcaacccc cctaaaaaat tttttttgag tttttatctc aagtgctgaa
      241 attatcagca aggcgttcaa tttagcgtca aaatcacgcc gccgcaagtg gattccgacg
      301 cttacgtgac caggaaaaat tttacagctt gataaaatgc tcgcggtagt aagccagctc
      361 cgccaccgat tcacggatat catccatcgc ctgatgcgtc ccctgcttgg taaaaccatc
      421 cagaatttcc ggcttccagc ggcgcgccag ctctttcagg gtgctgacat cgagataacg
      481 gtagtggaag taggcttcca gctccggcat gtatttaaac aggaagcgac gatcctggcc
      541 gatgctgtta ccgcaaatcg gcgattttcc cgcaggcacc cactgtttta agaattcgag
      601 cgttgccagt tcagcttcac gatcgcccat cgtgctcgct ttcacgcgct ctaccagccc
      661 gctggcggta tgggtgcgca cgttccagtc atccatcagc gccagctgtt catcggactg
      721 gtgtactgca atggtcggcc cttctgccag aatattcagg ttggcatcgg tcaccagcgt
      781 ggcaatctca ataatgcgat cgcgctcggg atccagaccg gtcatctcaa gatcgatcca
      841 aatcaggttg ttttcattgg cactcatgct attttccacc cattcacagt gactatattg
      901 atccacaatt gcgtgtatca tagatgtttt gcccatcagg ggcgaccagg agtctgtacg
      961 attgagtaaa aataaactct ccaaaggcca gcagcgccgc gtgaacgcca atcaccagcg
     1021 tcgtcttaaa acgtctaagg agaagcccga ctacgacgac aatctgtttg gcgagcctga
     1081 tgaaggtatc gtcatcagcc gctttggtat gcacgctgat gtggaatccg ccgatggcga
     1141 cgttcaccgc tgcaatattc gccgtaccat ccgttcgctg gtgaccggcg accgcgtagt
     1201 ctggcgtccg ggtaaaccgg cggcggaagg cgtaaatgtc aaagggatcg tggaagcggt
     1261 gcatgagcgc acctcggtgt tgacgcgtcc ggatttctac gacggcgtga aacctattgc
     1321 cgccaacatc gaccagattg tcattgtctc cgccattttg ccggagctgt cactcaatat
     1381 tatcgaccgt tacctggtgg cctgcgaaac cttgcagatt gagccgatta ttgtgctcaa
     1441 caagatagac ctgctggacg acgaaggcat ggcgttcgtc aacgaacaga tggatatcta
     1501 ccgcaatatc ggttatcgcg tattgatggt ttccagccat actcaggatg ggctaaaacc
     1561 gctggaagag gcgttgaccg ggcgcatcag catttttgcc gggcagtctg gcgtcggcaa
     1621 atccagcctg ctgaacgcgt tgctggggct gcaaaaagag atcctgacca acgatgtctc
     1681 tgacaactcg gggcttggtc agcacaccac gaccgccgct cggctgtatc acttcccgca
     1741 cggcggtgat gtgattgatt cccctggtgt gcgtgagttc ggcctctggc acctggagcc
     1801 ggaacaaatc actcagggct ttgtcgaatt ccatgactat ttaggtctgt gtaaatatcg
     1861 cgattgcaaa cacgataccg atccgggctg cgctatccgg gaagcggttg aggaagggaa
     1921 aatcgcggaa acccgtttcg aaaactatca ccgtattctg gaaagcatgg cgcaggtaaa
     1981 aacgcgtaaa aacttttctg atacggatga ctgacaatta agctaaccgt cgttagaatc
     2041 gtcccctttt tttcaggatc cggcatgtag gccggatcag gaacgacaaa acaatggcct
     2101 ggaggctacc ttgttaaatt catttaaact ttcgctacag tacattctgc cgaaactatg
     2161 gcttactcgc ctggcgggtt ggggcgcaag caagcgggca ggatggctga caaaactggt
     2221 tatcgatctg ttcgttaaat actacaaggt cgacatgaaa gaggcgcaaa agccggacac
     2281 cgccagctac cgcaccttta acgaattctt tgtccgtccg ctgcgtgacg aagtacgccc
     2341 aatcgatacc gatccgaatg tcctggtcat gcctgccgat ggcgttatca gccagttggg
     2401 taaaatcgaa gaagataaaa tcctgcaagc caaaggccac aactacagcc tcgaagccct
     2461 gctggcaggc aactatctga tggcggacct gttccgcaac ggtacgtttg tgaccactta
     2521 cctctccccg cgtgactacc accgcgtaca catgccgtgc aacggtattc tgcgtgagat
     2581 gatctacgtg ccgggcgatc tcttctccgt taaccatctc acggctcaga acgtgccgaa
     2641 tctgtttgcc cgtaacgaac gcgtgatctg cctgttcgat accgaatttg gcccaatggc
     2701 gcagattctg gtcggtgcga cgattgtagg cagcattgag actgtctggg cgggcaccat
     2761 tacgccgccg cgcgaaggca tcatcaagcg ctggacctgg cctgccgggg aaaacgacgg
     2821 ttctgtggcg ctgctgaaag gccaggaaat gggtcgcttt aaacttggct ctaccgttat
     2881 caacctgttt gcaccgggta aagtgaatct ggttgagcaa ctggaaagcc tgtctgtcac
     2941 gaaaattggc cagccgctgg cagtatctac cgaaaccttt gttacgccag acgctgaacc
     3001 agccccactt cctgctgaag agatcgaagc agaacacgac gccagcccat tggttgacga
     3061 taaaaaagac caggtctaat ccatcaaagg aaacgctgac gtgcgcctga ttatcacttt
     3121 tctgatggcc tggtgcctca gttggggggc gtacgccgcg acggcccccg atagcaaaca
     3181 aatcacccag gaactggagc aggcaaaagc ggcgaaaccc gcgcagccag aagtcataga
     3241 ggcactccag tctgccttaa atgcgcttga ggaacgaaaa ggttcccttg agcgcatcaa
     3301 gcaatatcaa gaagttattg ataattatcc gaaactctcc gctactctgc gcgcacaatt
     3361 aaacaacatg cgtgacgagc cgcgcagcgt gtcgccgggg atgtctaccg acgcgctgaa
     3421 tcaggaaatt ctccaggtca gcagtcagtt gctggataaa agccgtcagg cccagcaaga
     3481 gcaggagcgc gcccgcgaga ttgccgattc gctgaatcaa ctgccgcaac agcaaaccga
     3541 cgcccgccgt cagttaaacg agatcgagcg ccgcctggga acgcttaccg gcaatactcc
     3601 gctcaatcag gcacaaaatt tcgcgttgca gtctgactct gcacgtctta aggcgctcgt
     3661 tgatgaactg gagctggcgc agctgtctgc caataaccgc caggaattag cgcgcttgcg
     3721 ctctgagctg gctgaaaaag agagccagca actggatgcg tatttgcagg ccttgcgtaa
     3781 tcaattgaac agccaacgcc agcttgaggc agagcgggcg ctggaaagta ccgaattact
     3841 agcagaaaac agtgccgatt tgccgaaaga tatcgtcgcg caattcaaaa ttaaccgcga
     3901 actatcggca gcgctgaatc aacaggcgca gcggatggat ctcgttgcct cgcaacagcg
     3961 tcaggctgcc agccagacgt tacaggtccg gcaggcgttg aatacgctgc gtgaacagtc
     4021 gcaatggctg ggatcgtcca atctgctcgg tgaagcgttg cgggcgcagg tggcacggct
     4081 gccggaaatg ccgaaaccac aacagcttga taccgaaatg gcgcagttgc gtgtgcaacg
     4141 gttacgttat gaggatctgc ttaataaaca gccgctgcta cggcaaattc atcaggccga
     4201 cggtcagccg ctgaccgccg agcaaaaccg tattctggaa gcacaactac gcactcagcg
     4261 tgagttgctg aactcattgt tgcagggtgg cgacacacta ctgctggaac tgaccaagct
     4321 gaaagtctct aacgggcaac tggaggatgc gctgaaagag gtgaacgaag cgacgcaccg
     4381 ctatctgttc tggacctctg acgtgcgccc gatgaccatc gcctggccgc tggaaatcgc
     4441 ccaggatctg cgtcgtctca tttcgctgga caccttcagt cagttgggca aagccagtgt
     4501 gatgatgctg accagcaaag agacgatttt gccgctgttt ggcgcgttga ttctggtcgg
     4561 ttgcagtatt tactcgcgcc gctattttac ccgttttctc gaacgttcgg cggcgaaagt
     4621 cggcaaagtg actcaggatc acttctggct gacgttacgc actcttttct ggtcgattct
     4681 cgtcgcgtca ccgctgccgg tgctgtggat gacgctgggt tacggcttgc gcgaggcgtg
     4741 gccttatccg ctggcggtcg cgattggcga tggtgtaacg gccaccgtgc cgctgctgtg
     4801 ggtagtgatg atttgcgcca cctttgcccg cccgaacggc ttgtttatcg ctcattttgg
     4861 ctggccgcgc gaacgtgttt cccgtgggat gcgctactac ctgatgagca tcgggcttat
     4921 tgtgccactg attatggcgc tgatgatgtt cgataacctc gacgaccgcg aattttccgg
     4981 ttcgctggga cggctttgct ttatcctcat ttgcggtgcg ctggcggtgg tcaccctcag
     5041 cctgaaaaag gccgggatac cgctgtatct caacaaagag ggcagcggcg acaatattac
     5101 caaccatatg ttgtggaaca tgatgattgg cgcgccactg gttgccattc tggcgtcggc
     5161 ggtgggttat ctggcaacgg cacaggcgct gttagcgagg cttgaaacct cggttgccat
     5221 ctggttcctg ctactggtgg tttatcatgt tatccgccgc tggatgctga tccagcgccg
     5281 caggctggcg tttgaccggg cgaagcatcg ccgggcagag atgttagcgc aacgtgcacg
     5341 tggcgaagag gaagcgcatc atcacagtag cccggaagga gcaattgaag tcgatgaaag
     5401 cgaagtcgat ctcgatgcca ttagtgcgca atccttgcgg ctggtgcgct caattttgat
     5461 gttgatcgcc ctgctttcgg tcattgtgct gtggtcagaa atccattccg ctttcggctt
     5521 cctcgaaaat atttcgctgt gggatgtcac ttccacggta cagggcgtgg aaagtctgga
     5581 gccgattacc ctcggtgcgg ggctgattgc cattctggtg tttatcatca ccacgcagct
     5641 ggtgcgcaat ctgcccgcgc tgctggaact ggcgattttg cagcacctgg atttaacgcc
     5701 gggtaccggc tacgccatca ccaccatcac caaatatctg ctgatgctga ttggcgggct
     5761 ggtcggcttc tcaatgattg gtattgagtg gtcgaaattg cagtggctgg tcgccgcgct
     5821 cggtgttggt ctcggttttg gtttgcagga aattttcgcc aactttatct ctggcctgat
     5881 catcctgttc gaaaaaccga ttcgcattgg cgatacggta acgatccgcg atcttactgg
     5941 tagcgtgaca aaaattaaca cccgcgccac caccatcagc gactgggacc gtaaagagat
     6001 aatcgtgccg aacaaggcgt ttattaccga gcagtttatc aactggtcgc tctctgactc
     6061 ggtcacgcgc gtggtgttga cgattccggc ccctgccgat gccaacagtg aagaagtgac
     6121 ggaaatcctg ctcaccgcag cgcgtcgctg ctcgctggtg atcgacaacc cggcaccgga
     6181 agtcttcctg gtggatctgc aacaggggat tcagattttc gagctgcgta tttacgccgc
     6241 tgagatgggt caccgtatgc cgctacgcca tgagatccac cagctgattc tggctggctt
     6301 ccatgcccac ggtatcgata tgccattccc gcccttccag atgcgtctgg aaagcctcaa
     6361 cggtaaacaa acggggagaa cgctgacgtc tgcgggcaaa ggtcgtcagg cgggaagttt
     6421 gtaatgcatg aagctgccaa atgatgtcaa cgtggctcat ttagcagctt aacatcatct
     6481 tcagttatca ccggccctgg agagtatcat ccatacgcat tacttcctga caactcatcg
     6541 ccggagagac gatgagttgc aggatttcat atgatcagtc gagagctaac tcaggtttat
     6601 tcgccatgcg actgcaatag ttctggtaaa tcgccatcgc taacagcgag aagaagacag
     6661 ggccgccaat catccacaat gtgctgtccc agtctccggc ttcaaccaca ggttgaatga
     6721 tggtgaagac gttcgcaaat gtcaccacca gtacgacaac cactgtcgca atcattgccg
     6781 acatacgcgt tttgaaaatc acaaatggtc tgtcgagatc ctgacgtgct ttaaagaatg
     6841 ggaaagccag cgcgaggaac aggtaaggaa gcgtcataga cacgttcgcc atcagcgtca
     6901 gcttgttaaa gaacgccgat gcggtaccgc caccaaacga aaccagcagg atgaagacag
     6961 taaccaaccc gcactgcatc cacatggcga tagacggcat ccccatcgca ttcaggcgcg
     7021 tcatcggttc cggccacaac gctttcggcg tcccctggat gatggctttc agcggtgaat
     7081 agcaaagcgt aaagaacgca ccggtataag cgaggaacat cgaaagtccg gtaatacgcg
     7141 caaaccatac gcccagcgac aatgacgctt caggtgacaa atgcagtgcg ttacccagcg
     7201 tcaccccgag gctcttcatc agcacatagg taatattgcc aaggttaacg gaaccattac
     7261 ttaatacctg ctgccagttt gtgctgacgc cccataaaaa tattgccagc gaataaccga
     7321 ttgaaataac aatagcggca aaaacaatac ctttggcaaa gttcttttct ggattttccg
     7381 ttttatcgac cagaccacca accgcttcaa ttccgccata ggcaaaaata gcaaatacca
     7441 caaacgatag cattgccaga ccggactgat aacctgggtt cggtgatgca aggaaattaa
     7501 tatcctgcgc gaaatgccca ccatttaata acaaaatagt aatgcttact aacagcaata
     7561 ctaaattcag acacattact gcaatacccc ccaccgcagt aatgcgggca attttattaa
     7621 tcccctttga agcaacgacg gtgaccagaa tcatccatgc cactgccagc agaccaacca
     7681 cctgcgtagg ctccagtccg gcaatacgcc agtgctgggt catgtcgcta ccatagagga
     7741 atgttgagaa cggtacccaa actttcgcgg aggtgctcac catccagatg atataagagg
     7801 aaaaccacat aaacgtacca atgaaggcaa aacgtggtcc gacactatta ttcatccagg
     7861 aatagatacc gccttcttct ttgcgataag cagctcccat ttcagccatc attaaggcga
     7921 atggaataaa gaataacaat gcagaaaata tataaaaggg aatcgcacta taacccatta
     7981 agtaataagc cgatgggcta ttggcaaatc caaatacgga agtaaagatc atcaatatga
     8041 gtcctatcag actcatcttt tttatcgtgt gaggcatgaa accatccttc atttcagcgc
     8101 gtgtataaaa cgcgcccact gaactgcact gcccaacatt gagcgggcag cgtaaaaacc
     8161 aatacagaat tgatacctgg atgatatcaa atatccagtc ttaaattgtg gcctttagcg
     8221 gaaaaatgaa aacatatgct acagaatggc gcttatcacg ccattcttcg ctgttaattc
     8281 agtaattttt cagaattatg cccggtcaac gctaaaggcg atgacttcag ccagtgtctc
     8341 cgcacccagc gccaacatca ccagacgatc aacgcctaat gccacgccgg aacagtcagg
     8401 cataccgact ttcaaggctt caatcagatt ctggtcaatg gggtgctgcg gcaaaccgcg
     8461 cgccgcgcgc ttacggttat cttgttcaaa gcgttgttgc tgctcacggg catccgtcaa
     8521 ttcatggaaa ccattcgcca gctcaatacc tttataataa acctcaaagc gttcagcgac
     8581 ccgatgatct tcggtactga tttgcgccag tgatgcctgg ctggctggaa agtggtacac
     8641 aaaggtcggt ttttctttac caatatttgg ttctacgcca aaggtaaaca gcaattgtag
     8701 caacgtgtcg cggtcttctt cggtatcagc aacattgctc aaatccagtt tcgctgccac
     8761 ttcccgcagt tgcgttttgt cggcagagag cgggtcaatt tccagataac gcaagaaagc
     8821 ttgttgataa gaaaggcttt ctgctgccgg gcagtccagc acctgttgta agagatcgtc
     8881 cacctcgttc atcaaccggt acatatcata gtgcggtcga taccactcaa gcatagtgaa
     8941 ctcagggttg tgataacgcc ccatctcttc attacggaaa ctgcggcaca gctggaatac
     9001 cggcccgcaa ccggcaacca gcaggcgttt catatggtat tccgggctgg tcattaacca
     9061 gagattcatc ccctgcgaat gcccggggcc aacgaaacgt gtctcaaacg ggaccaaatg
     9121 aatatcggtt accgtcgcct ggctcataca aggcgtctcc acctccagca ctccacgatc
     9181 ggcaaagaaa cgacggatct ccgccataat cgccgcgcgt tttaataagt taggaatgga
     9241 tgcgctcggc tgccaggatg ccgtttcgct catagttaaa tctccagttt ttgacaaggg
     9301 cacgaagtct actcgcaacg cgacggcgag acaaatttta cgcaggaata aaacaacggt
     9361 gggcagtgac taaaaaaagc acgatctgat ggtttagtaa ttaaattaat catcttcagt
     9421 gataatttag ccctcttgcg cactaaaaaa atcgatctcg tcaaatttca gacttatcca
     9481 tcagactata ctgttgtacc tataaaggag cagtggaata gcgttcgcag accgtaactt
     9541 tcaggcactt accctgaagt acggggctgt gggataaaaa caatctggag gaatgtcgtg
     9601 caaacctttc aagccgatct tgccattgta ggcgccggtg gcgcgggatt acgtgctgca
     9661 attgctgccg cgcaggcaaa tccaaatgca aaaatcgcac taatctcaaa agtatacccg
     9721 atgcgtagcc ataccgttgc tgcagaaggg ggctccgccg ctgtcgcgca ggatcatgac
     9781 agcttcgaat atcactttca cgatacagta gcgggtggcg actggttgtg tgagcaggat
     9841 gtcgtggatt atttcgtcca ccactgccca accgaaatga cccaactgga actgtggggg
     9901 tgcccatgga gccgtcgccc ggatggtagc gtcaacgtac gtcgcttcgg cggcatgaaa
     9961 atcgagcgta cctggttcgc cgccgataag accggcttcc atatgctgca cacgctgttc
    10021 cagacctctc tgcaattccc gcagatccag cgttttgacg aacatttcgt gctggatatt
    10081 ctggttgatg atggtcatgt tcgcggcctg gtagcaatga acatgatgga aggcacgctg
    10141 gtgcagatcc gtgctaacgc ggtcgttatg gctactggcg gtgcgggtcg cgtttatcgt
    10201 tacaacacca acggcggcat cgttaccggt gacggtatgg gtatggcgct aagccacggc
    10261 gttccgctgc gtgacatgga attcgttcag tatcacccaa ccggtctgcc aggttccggt
    10321 atcctgatga ccgaaggttg ccgtggcgaa ggcggtattc tggtcaacaa aaatggctac
    10381 cgttatctgc aagattacgg catgggcccg gaaactccgc tgggcgagcc gaaaaacaaa
    10441 tatatggaac tgggtccacg cgacaaagtc tctcaggcct tctggcacga atggcgtaaa
    10501 ggcaacacca tctccacgcc gcgtggcgat gtggtttatc tcgacctgcg tcacctcggc
    10561 gagaaaaaac tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc gtacgttggc
    10621 gtcgatccgg ttaaagaacc gattccggta cgtccgaccg cacactacac catgggcggt
    10681 atcgaaaccg atcagaactg tgaaacccgc attaaaggtc tgttcgccgt gggtgaatgt
    10741 tcctctgttg ggctgcacgg tgcaaaccgt ctgggctcca actccctggc ggaactggtg
    10801 gtcttcggcc gtctggccgg tgaacaagcg acagagcgtg cagcaactgc cggtaatggc
    10861 aacgaagcgg caattgaagc gcaggcagct ggcgttgaac aacgtctgaa agatctggtt
    10921 aaccaggatg gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct ggctatggaa
    10981 gaaggttgcg gtatctaccg tacgccggaa ctgatgcaga aaaccatcga caagctggct
    11041 gagctgcagg aacgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt gttcaacacc
    11101 gacctgctct acaccattga actgggccac ggtctgaacg ttgctgaatg tatggcgcac
    11161 tccgcaatgg cacgtaaaga gtcccgcggc gcacaccagc gtctggacga aggttgcacc
    11221 gagcgtgacg acgtcaactt cctcaaacac accctcgcct tccgcgatgc tgatggcacg
    11281 actcgcctgg agtacagcga cgtgaagatt actacgctac cgccagctaa acgcgtttac
    11341 ggtggcgaag cggatgcagc cgataaggcg gaagcagcca ataagaagga gaaggcgaat
    11401 ggctgagatg aaaaacctga aaattgaggt ggtgcgctat aacccggaag tcgataccgc
    11461 accgcatagc gcattctatg aagtgcctta tgacgcaact acctcattac tggatgcgct
    11521 gggctacatc aaagacaacc tggcaccgga cctgagctac cgctggtcct gccgtatggc
    11581 gatttgtggt tcctgcggca tgatggttaa caacgtgcca aaactggcat gtaaaacctt
    11641 cctgcgtgat tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg
    11701 cgatctggtg gtcgatatga cccacttcat cgaaagtctg gaagcgatca aaccgtacat
    11761 catcggcaac tcccgcaccg cggatcaggg tactaacatc cagaccccgg cgcagatggc
    11821 gaagtatcac cagttctccg gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca
    11881 gtttggcctg aacccagagt tcatcggtcc ggctgccatt acgctggcgc atcgttataa
    11941 cgaagatagc cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca gccagaacgg
    12001 cgtatggagc tgtactttcg tgggctactg ctccgaagtc tgcccgaaac acgtcgatcc
    12061 ggctgcggcc attcagcagg gcaaagtaga aagttcgaaa gactttctta tcgcgaccct
    12121 gaaaccacgc taaggagtgc aacatgacga ctaaacgtaa accgtatgta cggccaatga
    12181 cgtccacctg gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc gaaggcacgg
    12241 cggttccggc tgtgtggttc agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg
    12301 gcccggaagc ctgggcggga ttcgtcgact ttttacaaaa cccggttatt gtgatcatta
    12361 acctgatcac tctggcggca gctctgctgc acaccaaaac ctggtttgaa ctggcaccga
    12421 aagcggccaa tatcattgta aaagacgaaa aaatgggacc agagccaatt atcaaaagtc
    12481 tctgggcggt aactgtagtt gccaccatcg taatcctgtt tgttgccctg tactggtaag
    12541 gagcctgaga tgattaatcc aaatccaaag cgttctgacg aaccggtatt ctggggcctc
    12601 ttcggggccg gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct gctggttggt
    12661 attctgctgc cgctggggct gtttccgggt gatgcactga gctacgagcg cgttctggcg
    12721 ttcgcgcaga gcttcattgg tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg
    12781 tgtggtttac accgtatgca ccacgcgatg cacgatctga aaatccacgt acctgcgggc
    12841 aaatgggttt tctacggtct ggctgctatc ctgacagttg tcacgctgat tggtgtcgtt
    12901 acaatctaac gcatcgccaa tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga
    12961 aaccagaccc tatgttcaaa acgacgctct gcgccttatt aattaccgcc tcttgctcca
    13021 catttgctgc ccctcaacaa atcaacgata ttgtgcatcg cacaattacc ccgcttatag
    13081 agcaacaaaa gatcccgggt atggcggtgg cggtaattta tcagggtaaa ccttattact
    13141 ttacctgggg ctatgcggac atcgccaaaa agcagcccgt cacacagcaa acgttgtttg
    13201 agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct attgctcgag
    13261 gggaaatcaa gttaagcgat cccgcaacaa aatactggcc tgaacttacc gctaaacagt
    13321 ggaacgggat cacgctatta catctcgcaa cctacaccgc tggcggcctg ccattgcagg
    13381 tgccggatga ggtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg
    13441 catgggcgcc aggaacacaa cgtctgtatg ccaactccag tatcggtttg ttcggcgcac
    13501 tggctgtgaa gccgtctggt ttgagttttg agcaggcgat gcaaactcgt gtcttccagc
    13561 cactcaaact caaccatacg tggattaatg taccgcccgc agaagaaaag aattacgcct
    13621 ggggatatcg cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat gctgaaactt
    13681 atggtgtgaa gtcgaccatt gaagatatgg cctgctgggt acgaagcaat atgaatcccc
    13741 gtgatatcaa cgacaaaaca cttcagcaag ggatacaact ggcacaatct cgctactggc
    13801 aaaccggcga tatgtatcag ggcctgggct gggaaatgct ggactggccg gtaaatcctg
    13861 acagcatcat taacggcagt gacaataaaa ttgcactggc agcacgcccc gtaaaagcga
    13921 ttacgcctcc aactcctgca gtacgcgcat catgggtaca taaaacgggg gcgaccggcg
    13981 gatttggtag ctatgtcgcg tttattccag aaaaagagct gggtatcgtg atgctggcta
    14041 acaaaaacta tcccaatcca gcgagagtcg ccgccgcctg gcagattctc aacgctctgc
    14101 agtaaaattt catcgggtcc gaattttcgg accttttctc cgcttttcct tgctgtcatc
    14161 tacacttaga aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca
    14221 gcgacagctg catttctggt cgttgcctgc agttctccta cgccgccgcg tggcgtgacc
    14281 gtagtgaata atttcgacgc caaacgttat ctcggtacct ggtatgagat tgcccgtttt
    14341 gatcaccgct ttgagcgtgg actggaaaaa gtcaccgcaa catacagcct gcgtgatgac
    14401 ggcggcctga atgtcattaa taaaggctat aaccctgaca gaggaatgtg gcagcagagt
    14461 gaagggaaag cgtactttac cggcgcacca actcgcgctg cgctgaaagt gtcattcttt
    14521 ggtcctttct atggcggtta taacgttatt gcactcgatc gggaataccg ccatgcgctg
    14581 gtttgcggcc cggaccgcga ctacctgtgg atactctccc gcacgccaac catttctgac
    14641 gaagtgaaac aggagatgct ggcagtcgcg acccgggaag ggtttgatgt cagtaaattt
    14701 atttgggtac agcagcccgg cagttagtga gtgctgagtt tcagaccaat aatccccaat
    14761 acgattaacg ccagactcgc caggcgcatc gggttagcgg actcaccgag cagcacaatg
    14821 ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcataagc cgtccctact
    14881 ggtaacgatt tcatcgccca ggcaagtagc gccatactga caatcatcgc cgtcacggta
    14941 ataacactcg gcgtcaaacg actaaagccg tgggtatatt tcaggccaac ggcccatacc
    15001 acttccagca gaccagcaat aactaagata atccaggaca tatcaggctc cggagaattg
    15061 gggccgtccc cggtgaaaag acgcgtttgc aggtcgtcct gcaaagctga tggttgaacg
    15121 gatattttgc caggtggcag gggaatttca actattacct ttgtgactat cgcagagaaa
    15181 acaaacggca cgacacagct gtcgtaccgt tattgcttat tgctgcgctt tcgttgctgc
    15241 accagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg tggtgttgca
    15301 ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg tcttcttcac
    15361 cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat taataataga
    15421 caacatcgcc aaaaatgatg gaaatctgat gatttttcgg aagagaagaa aaattagctg
    15481 gcagcgcggg agatggagtt gccgagatgt ttgatgtctt cgccgaaacc gcgagcggtg
    15541 ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg tttcatcatc
    15601 tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca ttacttcacg
    15661 cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat ttgtacgaac
    15721 agcggaactt taaccacagc gccagtagac agggtagccg gtttgccgcc agtacctgcg
    15781 gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa gttcggcgga
    15841 gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg atccagcagc
    15901 catttcgcgt tgtcgccaat tgcttttgcg tcagcagaca gctgctcgaa agtttcgttg
    15961 ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat atcgacaaca
    16021 tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt accggtcagc
    16081 agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac gaattcactc
    16141 gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg aaaatcgttg
    16201 ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa gccacaaaat
    16261 ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc aacttgccga
    16321 tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg acgaaaaact
    16381 gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct catttatcga
    16441 tcgcatggag aaaggcaatc cggacgatcc gcttttgcgt caggtaatta cctcgcaaga
    16501 tgagtttgcc atcgcgcccg gattctccac cgacccgctg gaagaacagc acagcgtagt
    16561 gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag gcggctgcgc
    16621 ggtaaattgc cgttattgct tccgtcgcca cttcccttat gcagaaaatc agggcaacaa
    16681 gcgtaactgg caaactgcac ttgagtatgt tgctgcgcat ccggaactgg acgagatgat
    16741 tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt tgctcacaca
    16801 actggaagcc atcccgcata tcaaacgtct gcggattcac agtcgtctgc cgattgtgat
    16861 cccggcacgc atcacagatg cgctggttga acgcttttca cattctacgc tgcaaatctt
    16921 gctggtgaat cacatcaacc atgccaatga gatagatgaa acattccgtc aggcgatggc
    16981 taagttgcgc cgggtaggcg ttactttgct gaaccagagc gttctgttac gtgatgtgaa
    17041 cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg gcgtgatgcc
    17101 gtattacctg catgtgctcg ataaagtaca gggtgcggcg cattttatgg tgagtgatga
    17161 cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggatatc tggtgccgaa
    17221 actggcgcga gaaattggcg gcgaacccag caaaacgccg ctggatctcc agctacgcca
    17281 gcagtaactg attaaacagg gaagtcaaac attaaaattg cctgatgcgc tacgcttatc
    17341 aggcctacat cgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
    17401 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaacagg
    17461 gaagtttaat ctttgcccgt tgcataacct ttaattattc atatgcattg ctgttgttac
    17521 tctttatata actaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
    17581 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
    17641 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
    17701 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
    17761 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
    17821 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact ggatgctgct gcacgccttc
    17881 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaatatc ccagaaattc
    17941 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    18001 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    18061 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    18121 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    18181 acgacgttga ctgccagcaa aatggcaatc tggaatatga cacctattcc caaccggaat
    18241 ggaaacataa cttgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    18301 gcaaagaaca atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    18361 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    18421 tgccatgtca ggtctatgtt cgcaccgtag cagcaaataa cgcccaaccg ctaacccagg
    18481 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    18541 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    18601 ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc cttatccggc ctacaaattc
    18661 tcaagcattc aatcagttgc aatgcattat gtaggcctga taagcgaagc gcatctggca
    18721 attatacgtt tgtcaccatc acatcagttc gggcacttat atacctggcc gataatctgg
    18781 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctactgat gccataaatc
    18841 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
    18901 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
    18961 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
    19021 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
    19081 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgtcac tcgcgtcgtc
    19141 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
    19201 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
    19261 tgccgcccat accgccagca gcgcctaagt cagctgcatc gtttttcggc aggtcggtaa
    19321 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
    19381 gggttacttt ggttggatcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
    19441 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
    19501 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
    19561 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
    19621 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
    19681 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
    19741 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ctcctgcagt ttttcacggt
    19801 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
    19861 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
    19921 tagcctgacc caggtcttcc agggttgctt tttccagctc cataccgatc tcttcagaga
    19981 ttacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
    20041 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
    20101 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
    20161 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
    20221 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
    20281 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
    20341 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
    20401 gtttacctac ggtttcgtcg gagttagcgg agatagtacc aacctgagca atcgctttag
    20461 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acagctttgt
    20521 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccttcag
    20581 tgatgatagc ctgagccagt acggttgcag tggtggtacc gtcgcctgca gcgtcgttcg
    20641 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
    20701 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
    20761 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
    20821 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
    20881 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
    20941 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcggatttc
    21001 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
    21061 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
    21121 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
    21181 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
    21241 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
    21301 atggggatga ggcttcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
    21361 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
    21421 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
    21481 cggacatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
    21541 gctatcgacg tcattattag gcactggcgt gtttgccgtt cctgcgttag ctgcactggt
    21601 agcgggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
    21661 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggcgtcg cgcacttcgt
    21721 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggctgtttt tatcggtcat
    21781 tcccgtgggt ttgcctgccg cgctacaaat tgctgccgga ttcggccagg caatgtttgg
    21841 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgttgg tgtggtatat
    21901 cggtactcgc ggtgccagtt ccagtgcaaa tctacaaacc gttattgccg gacttatcgt
    21961 cgcactgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgagta tccccttccc
    22021 cgcgccagga aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
    22081 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagaacgtga
    22141 ttttcctcgt gctttgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
    22201 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
    22261 aaaaattgta gtacaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
    22321 tctggcctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
    22381 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
    22441 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc acgttggtga ttcatgcttt
    22501 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
    22561 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
    22621 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
    22681 gatcatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
    22741 aaccacataa tccggcgttt cgacattaat cctggcgatc gtctttatga tcaaggcggt
    22801 cgcgctcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
    22861 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
    22921 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
    22981 gtaataaaag cagcaaaccg gcaatgatca gcgaaacact tttaatcatc tccgccgctg
    23041 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
    23101 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc aacaataccc
    23161 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
    23221 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
    23281 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
    23341 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
    23401 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
    23461 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
    23521 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
    23581 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
    23641 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
    23701 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
    23761 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
    23821 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
    23881 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
    23941 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
    24001 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
    24061 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
    24121 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
    24181 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
    24241 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
    24301 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagttactg gcttcccatg
    24361 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
    24421 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
    24481 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
    24541 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
    24601 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
    24661 gaacgttatg gagccggtca ttggccaggc catgttcgaa tccgttcaca ttctgaccaa
    24721 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
    24781 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
    24841 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
    24901 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
    24961 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
    25021 cagggtagta caaataaaaa aggcacgtca gatgacgtgc cttttttctt gtgagcagta
    25081 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
    25141 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
    25201 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
    25261 taacatccca ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
    25321 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
    25381 cccgaaatac atcacgatcc tcgcaccgat cgtgacctat ttcctgacta tctttgctgg
    25441 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
    25501 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
    25561 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
    25621 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
    25681 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
    25741 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
    25801 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
    25861 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
    25921 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
    25981 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
    26041 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
    26101 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
    26161 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
    26221 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
    26281 ctacccgacg ctggttgctg cggtacagat ggatgacacg ggtactaccc gtatcggtaa
    26341 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
    26401 cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc gttcacgccc
    26461 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
    26521 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
    26581 ctgtcgtggt gctatgtacg gcaccagatg aagcgacagc ccaggattta gccgccaaag
    26641 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
    26701 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
    26761 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
    26821 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
    26881 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
    26941 acgttcacaa tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
    27001 tgaccttaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
    27061 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
    27121 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
    27181 aaaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
    27241 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
    27301 agcgtcacag cctgtgtctg tttcgcagca agagcagcac accgcgcaat tgcccttttc
    27361 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
    27421 gtacccactg atttctggca tcgtgctggg cggtaaacag cggctctcca ctgccagagc
    27481 attgttgctg acctttattt atgtgcaggg gatggcgctg acctacacgg cgctgggtct
    27541 ggtggttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
    27601 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
    27661 actcccctcc tcgctgcaaa cgcgcctcac gctgatgagc aatcgccaac agggcggctc
    27721 acccggcgga gtgtttatta tgggggcgat tgccggactg atctgttcac catgcaccac
    27781 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    27841 cggcacgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    27901 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    27961 ttttgtgatc ctcgcactgc cggtcttcct gctggagcga gtgattggtg atatatgggg
    28021 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    28081 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    28141 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    28201 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    28261 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaagagtt
    28321 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    28381 tcaggccaac gtcacggcca acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    28441 ccttggccta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    28501 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    28561 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    28621 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    28681 tgaacgtgtg gattatccac tggacgagct gcgccgcttc tggccagaca aagaggcaat
    28741 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    28801 gctggacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    28861 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    28921 cgatcctggc caccctattc atcaactggc cgatcagcaa aaaagcgcag cctacgattt
    28981 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    29041 ggaactggtg ctggaaggct gtttaagccg aatgctagtg aatcgtagcc aggcggatgt
    29101 cgacactgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    29161 cgcattgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    29221 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    29281 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    29341 cgagtccggg caccaaattc atatcaacgg acctccacgg aggtccgttt ttcgtttcag
    29401 aacaccatga tttaagtatt cttcctccaa atcaattcta ccgaactcaa ccaaattctc
    29461 cccacatcaa cctcattgtg tgggtataat tgcgggtata ccccagttcg acagaatttg
    29521 taccctcttt aacgcattca gaaggctgaa ctatggcact gactgacgca aaaatccggg
    29581 ctgcaaagcc cactgacaag gcttataaac tcactgacgg ggctggcatg ttcctgctgg
    29641 tacatcctaa tggttcccgt tactggcgtc tccgttatcg tattctgggt aaagagaaga
    29701 ctctggcact tggtgtgtat ccagaagtat ctctctccga agctcgtaca aaacgggatg
    29761 aggcccgaaa actgatttcg gagggggttg acccttgcga acagaaaaga gctaaaaaag
    29821 tagtccctga tttacaactc tctttcgaac atattgcacg acgctggcat gccagtaata
    29881 aacaatgggc acaatcacac agcgataaag tactcaaaag cctcgagaca cacgttttcc
    29941 cctttatcgg caaccgggat atcacaacac tcaatacccc ggacctgctt atccctgttc
    30001 gtgctgcaga agcaaaacaa atttatgaaa tcgccagtcg tctgcagcaa agaatatctg
    30061 ctgtaatgcg ttacgccgta cagtctggca tcatcagata taacccggct ctggatatgg
    30121 ctggtgcatt gaccacggta aaacgccagc atcgccccgc tctgaatctt tcacgcctgc
    30181 ctgaacttct gtcgcgtatt gacggttata aaggccagcc tgtcacccgg cttgccgtta
    30241 tgctgaattt actggttttt attcgttcca gtgaactcag atatgcccgc tggtctgaaa
    30301 ttgatattga caatgctatg tggactattc tagccgaacg cgaacctctg cctgccgtaa
    30361 aattctcaca ccggggctcc aagatgcgaa caccacatct tgtgccactc agcaaacagg
    30421 ctgtagccat actgacagaa cttcagacat gggctggtga aaatggtctg atctttacgg
    30481 gagcacatga cccgcgtaaa ccaatc
//
LOCUS       DAZGCQ010000057        30027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000057 DAZGCQ010000000
VERSION     DAZGCQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.057"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(412..2241)
                     /gene="glmS"
                     /locus_tag="ACHK07_002814"
     CDS             complement(412..2241)
                     /gene="glmS"
                     /locus_tag="ACHK07_002814"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO8103124.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2403..3773)
                     /gene="glmU"
                     /locus_tag="ACHK07_002815"
     CDS             complement(2403..3773)
                     /gene="glmU"
                     /locus_tag="ACHK07_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO8103125.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELQEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(4125..4544)
                     /gene="atpC"
                     /locus_tag="ACHK07_002816"
     CDS             complement(4125..4544)
                     /gene="atpC"
                     /locus_tag="ACHK07_002816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO8103126.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4565..5947)
                     /gene="atpD"
                     /locus_tag="ACHK07_002817"
     CDS             complement(4565..5947)
                     /gene="atpD"
                     /locus_tag="ACHK07_002817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO8103127.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRCLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5974..6837)
                     /gene="atpG"
                     /locus_tag="ACHK07_002818"
     CDS             complement(5974..6837)
                     /gene="atpG"
                     /locus_tag="ACHK07_002818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO8103128.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6888..8429)
                     /gene="atpA"
                     /locus_tag="ACHK07_002819"
     CDS             complement(6888..8429)
                     /gene="atpA"
                     /locus_tag="ACHK07_002819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO8103129.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8442..8975)
                     /gene="atpH"
                     /locus_tag="ACHK07_002820"
     CDS             complement(8442..8975)
                     /gene="atpH"
                     /locus_tag="ACHK07_002820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO8103130.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8990..9460)
                     /gene="atpF"
                     /locus_tag="ACHK07_002821"
     CDS             complement(8990..9460)
                     /gene="atpF"
                     /locus_tag="ACHK07_002821"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO8103131.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9522..9761)
                     /gene="atpE"
                     /locus_tag="ACHK07_002822"
     CDS             complement(9522..9761)
                     /gene="atpE"
                     /locus_tag="ACHK07_002822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO8103132.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9808..10623)
                     /gene="atpB"
                     /locus_tag="ACHK07_002823"
     CDS             complement(9808..10623)
                     /gene="atpB"
                     /locus_tag="ACHK07_002823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO8103133.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10632..11012)
                     /gene="atpI"
                     /locus_tag="ACHK07_002824"
     CDS             complement(10632..11012)
                     /gene="atpI"
                     /locus_tag="ACHK07_002824"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO8103134.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11629..12252)
                     /gene="rsmG"
                     /locus_tag="ACHK07_002825"
     CDS             complement(11629..12252)
                     /gene="rsmG"
                     /locus_tag="ACHK07_002825"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO8103135.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12316..14205)
                     /gene="mnmG"
                     /locus_tag="ACHK07_002826"
     CDS             complement(12316..14205)
                     /gene="mnmG"
                     /locus_tag="ACHK07_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO8103136.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14584..15027)
                     /gene="mioC"
                     /locus_tag="ACHK07_002827"
     CDS             complement(14584..15027)
                     /gene="mioC"
                     /locus_tag="ACHK07_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO8103137.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(15117..15575)
                     /gene="asnC"
                     /locus_tag="ACHK07_002828"
     CDS             complement(15117..15575)
                     /gene="asnC"
                     /locus_tag="ACHK07_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO8103138.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15727..16719
                     /gene="asnA"
                     /locus_tag="ACHK07_002829"
     CDS             15727..16719
                     /gene="asnA"
                     /locus_tag="ACHK07_002829"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO8103139.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16724..18175)
                     /gene="viaA"
                     /locus_tag="ACHK07_002830"
     CDS             complement(16724..18175)
                     /gene="viaA"
                     /locus_tag="ACHK07_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO8103140.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSKEWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(18169..19665)
                     /gene="ravA"
                     /locus_tag="ACHK07_002831"
     CDS             complement(18169..19665)
                     /gene="ravA"
                     /locus_tag="ACHK07_002831"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO8103141.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQACAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19887..21755
                     /gene="kup"
                     /locus_tag="ACHK07_002832"
     CDS             19887..21755
                     /gene="kup"
                     /locus_tag="ACHK07_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO8103142.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21922..22341
                     /gene="rbsD"
                     /locus_tag="ACHK07_002833"
     CDS             21922..22341
                     /gene="rbsD"
                     /locus_tag="ACHK07_002833"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO8103143.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22349..23854
                     /gene="rbsA"
                     /locus_tag="ACHK07_002834"
     CDS             22349..23854
                     /gene="rbsA"
                     /locus_tag="ACHK07_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO8103144.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23859..24824
                     /gene="rbsC"
                     /locus_tag="ACHK07_002835"
     CDS             23859..24824
                     /gene="rbsC"
                     /locus_tag="ACHK07_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO8103145.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24849..25739
                     /gene="rbsB"
                     /locus_tag="ACHK07_002836"
     CDS             24849..25739
                     /gene="rbsB"
                     /locus_tag="ACHK07_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO8103146.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25865..26794
                     /gene="rbsK"
                     /locus_tag="ACHK07_002837"
     CDS             25865..26794
                     /gene="rbsK"
                     /locus_tag="ACHK07_002837"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8103147.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLKSVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26798..27790
                     /gene="rbsR"
                     /locus_tag="ACHK07_002838"
     CDS             26798..27790
                     /gene="rbsR"
                     /locus_tag="ACHK07_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO8103148.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27756..29183)
                     /gene="mdtD"
                     /locus_tag="ACHK07_002839"
     CDS             complement(27756..29183)
                     /gene="mdtD"
                     /locus_tag="ACHK07_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO8103149.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(29206..29898)
                     /gene="yieP"
                     /locus_tag="ACHK07_002840"
     CDS             complement(29206..29898)
                     /gene="yieP"
                     /locus_tag="ACHK07_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO8103150.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7267 a         7652 c         7645 g         7463 t
ORIGIN      
        1 tttcatattt acataatcca caactatgaa tttgcgtaga aaacagtctt atttttagta
       61 aaccacacat tcaaattata ttttttgcct atatttgtgg aaagactata tatttacagt
      121 gataatttat tatcacacat tgttacatag ttaaataaaa ttacatattg atgattgttt
      181 tacaagcgat tcttgaatga acaaaacgtg ctattcatta caaagtacgt caattatggc
      241 aattgtttgt caatgattcg aggtatttaa ttactactac ggttaaaaca agtgttcaac
      301 ggtgccccaa ataatagctg aagttaatta tatggaatga ggttagatta ttaatgctgg
      361 taagtaaaag ggccgccaaa aggcagccct tcgttcagca aatgaataat attactcaac
      421 cgttaccgat tttgccaggt tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac
      481 gtgataagcc agtaactgca gcggaacggt gtagaagatc ggtgcaatca cctcttccac
      541 atgcggcatc tcgatgatgt gcatgttatc gctacttaca aaacccgcat cctgatcggc
      601 gaagacatac aattggccgc cacgcgcacg aacttcttca atgttggatt tcagtttttc
      661 cagcaattcg ttgttcggcg caacaacgat aaccggcata tccgcgtcaa tcagcgccag
      721 cggaccgtgt ttcagttcac ctgcagcgta ggcttcagcg tgaatgtaag agatctcttt
      781 cagcttcaat gcgccttcca gcgcgattgg gtactgatcg ccacggccca ggaacagcgc
      841 gtgatgtttg tcagagaaat cttctgccag agcttcaatg cgtttgtcct gagacagcat
      901 ctgctcaata cggctcggca gcgcctgcag accatgcaca atgtcatgtt caatggaggc
      961 atccagacct ttcaggcgag acagcttcgc caccagcatc aacagcacag ttaactgggt
     1021 ggtgaatgct ttagtggatg ccacgccgat ttctgtaccc gcgttggtca ttagcgccag
     1081 atcggattcg cgcaccagag aagaacccgg aacgttacag attgccagtg aaccaaggta
     1141 acccagctct ttcgacagac gcaggcccgc cagggtatct gcggtttcgc cagactgtga
     1201 caaggtgatc atcaggctgt tacgacgcac ggcagatttg cgatagcgga attcagaggc
     1261 gatttcgacg tcgcacggaa tacctgccag cgattcaaac cagtaacggg aaaccatacc
     1321 ggagttataa gaagtaccac aggcgaggat ctgaatatgc tcaaccttcg acagcagttc
     1381 gtcggcgttc ggtcccagct cgcttaaatc aacctgaccg tggctgatgc gcccggtaag
     1441 ggtgtttttg atcgcgttcg gctgttcgta gatctctttc tgcatgtagt gacggtaaat
     1501 acctttatcg cccgcgtcat attgcagatt ggattcgata tcctgacgtt ttacttccgc
     1561 gccagtttta tcgaagatgt ttaccgaacg gcgagtaatt tccgcaatat cgccctcttc
     1621 aaggaagata aagcgacggg tcaccggcag cagcgccagc tggtcagaag cgataaagtt
     1681 ttcgcccata cccagaccaa tcaccagcgg actaccagaa cgtgctgcca gcagggtatc
     1741 cgggtgacgg gagtccatga tcactgtacc gtacgcacca cgcagttgcg ggatagcacg
     1801 cagaacagcc tcacgcagag taccgccctg tttcagctcc cagttcacca gatgggcaat
     1861 cacttcggtg tcggtttcag aaacgaaggt atagccacgc gcttttaact cttcacgcag
     1921 cggttcatgg ttttcgatga tgccgttatg caccaccaca atgtgttcag aaacatgcgg
     1981 atgcgcgtta acttctgaag gttcaccatg ggtcgcccag cgagtgtgag caataccggt
     2041 gccgccatgc agaggatgtt cttccgctgc ctgagccagc atctggactt taccgaggcg
     2101 acgcaggcgg gtcatatgac cttccgcatc aacaacggcc agaccggcag agtcatatcc
     2161 gcggtattcc agacgacgta aaccttcaag aaggatttct gctacatcac gttgcgcgat
     2221 cgcgccaaca attccacaca tagtttttga ttccgattta tatcgttgtc ggtcaacctg
     2281 tatgcccgtt ttttcgggcg ccccgagcct tgtagagagt ggggttattt ttatagttac
     2341 tgcttgtggg agggagatta tgttatctcc tcatcccatg tgaccggatt acccggccag
     2401 aatcactttt tctttaccgg acgacgccag ccttctttct gagtctgcgg cacacggctg
     2461 atagctaatg cattttcgcc gacattacgc gtcacagttg tacccgcagc aatggtcgcg
     2521 cctttgccta ctgttaccgg ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg
     2581 ccgataatgg tcttaaattt attcgcacca tcgtagttgc aggtaatggt tcccgcgccg
     2641 atgttaacgt tatcgccaat ttccgcatcg cccaggtaag tcagatgacc agctttcgag
     2701 cctttaccca gacgcgcttt tttcatctca acaaagttac cgacgtgtgc accttcctgc
     2761 aactcagcac caggacgcaa acgggcaaac gggccaatgg tacaggccgc cgccagattc
     2821 gcgtcttcca cgacggtata tgggctaatt tcgcaatcat cgccaatcac gctgttttta
     2881 atcacgcaac cggtgccgat tttcacgcga tgaccgagag tcacgttgcc ctcgatgata
     2941 acgttagtat caatttcaac atcgcgcccg tgagtaagcg taccacgcag atcaaaacgc
     3001 gccggatcgc gcagcataac gcctgctaac agcagttttt cagcctgttc ggactgataa
     3061 acacgctcca gacgggagag ttgcaggcgg ttattcacgc cttctacttc gcttaaacgt
     3121 tgcggatgaa cggcgacgat ttcacgccct tcctgatacg ccagtgcaat aatgtcggtg
     3181 atgtagtatt cgccctgagc attattgttg gtcagcttcg ccagccagcg tttcatatct
     3241 gcgccgttag cgatcagaat gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg
     3301 gcatctttgt gctcaacaat gccggtaact ttgccgtttt cacgggtgat acgtccataa
     3361 ccggtcggat catccagttt caccgtcagc agaccaatgc caccctgcgg tttagcatca
     3421 cgcagacgct ggagtgtttc gacagagatc agcggcacgt cgccgtagag cattaaaatg
     3481 tcttcatcat cggcaaagaa aggtgcagcc tgctgcattg catgaccagt acccagctgt
     3541 tctgcctgaa gcacccagtt caggttgtcg tctttcagcg cctgttttag cagatcgccg
     3601 ccgtgaccgt acaccaggtg aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca
     3661 tgctgaacca tcgctttccc ggcaagggta tgcagcactt tcggaagatc ggaatacatg
     3721 cgcgtgcctt tacctgcggc aaggatcact acgctcatag cattattcaa catacgcgtc
     3781 ctgactgtaa tttgagaacg aatttaaacc gcttcacctt gaaaaaacta catttttttc
     3841 atcgtgaaat ggacagagga taaattgttc aataacggat tatccccgct gacgaaagcg
     3901 ccattttcga ccatcgtcac gccttttgtc tgcagaaaat taaggcagta ccttcattta
     3961 agcgtcaggt ggatgttttt gctcttattt cgatcaatga ataaacggga aaacaggggg
     4021 ttttatatca gcaggatcta tgtgaacgct attcaggacg ggtcacacgc gcaaaaaaaa
     4081 gccagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg
     4141 gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg
     4201 taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg
     4261 gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg
     4321 ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt
     4381 ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag
     4441 atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt
     4501 tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa
     4561 ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg
     4621 cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt
     4681 ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct
     4741 gggacaggaa gcgctggatc ttacgagcac gtgctaccac cagtttgtct tcttcagaca
     4801 gttcatccat acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg
     4861 actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac
     4921 ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc
     4981 tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt
     5041 catccgcagg tacgtatact gcctgtacgg aggtgataga accggttttg gtggaggtga
     5101 tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag
     5161 gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt
     5221 caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg
     5281 tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata
     5341 ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac
     5401 gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga
     5461 gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc
     5521 ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag
     5581 agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac
     5641 cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg
     5701 ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga cagcgacgca
     5761 gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa
     5821 cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta
     5881 cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag
     5941 tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat
     6001 ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc
     6061 tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg
     6121 ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag
     6181 cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt
     6241 cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga
     6301 catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta
     6361 ggcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat
     6421 gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga
     6481 gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag
     6541 cagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac
     6601 caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttgt attccagatt
     6661 accgtgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc
     6721 catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat
     6781 cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag
     6841 cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg
     6901 gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca
     6961 ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga
     7021 gcggcttcga agctgccgat tttcgacagt tcaacatccg ccaggtaacc acgttctgct
     7081 gcgaacagaa ccagagactg ttgcgctacg gacatcggcg catactgttt ctgtttcagc
     7141 agttcggtca ctttctgacc gtggtcaagt tgcttacgtg ttgcatcgtc aaggtcggat
     7201 gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg
     7261 gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc
     7321 gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca
     7381 tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg
     7441 ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct
     7501 tcaacgtatt cggcgttaac acgcgcagca cgctccagca gacgagagtg gaggtagaat
     7561 acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg
     7621 taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca
     7681 cggaagtatt cgcccattgc gcaaccggca tacggtgcca ggtattgcag tgcagcggat
     7741 tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta
     7801 cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata
     7861 ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc ggtctgacgg
     7921 tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta
     7981 taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct
     8041 tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga
     8101 gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta
     8161 cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca
     8221 gagtcgcgct cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca
     8281 ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata
     8341 gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt
     8401 tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct
     8461 gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg
     8521 ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga
     8581 cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa
     8641 atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaga
     8701 acatccggga gcgcgttaag acgaccattt tcagccatta cccgaataag gttctgaccg
     8761 ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcaagcgt ttctggcgca
     8821 agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc
     8881 agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg
     8941 gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag
     9001 cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct
     9061 tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt
     9121 tacgctcggc ttcaatttcc gcctgcgcct gggccacgat tttagtacgt tcctgttctg
     9181 cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta
     9241 cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa
     9301 ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt
     9361 cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa
     9421 acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag
     9481 ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc
     9541 acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata
     9601 aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat
     9661 ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca
     9721 gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt
     9781 cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac
     9841 gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc
     9901 ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa
     9961 aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga
    10021 cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg
    10081 ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag
    10141 aatcaggata aatacgccca gtgccataga cagcgttacg ttcacgtccg cagacggaac
    10201 cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg
    10261 cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc
    10321 aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac
    10381 cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct
    10441 acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt
    10501 gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc
    10561 cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc
    10621 catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc
    10681 accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc
    10741 accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc
    10801 acccggcctt tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg
    10861 ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg
    10921 aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga
    10981 gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag
    11041 gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt
    11101 caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg
    11161 tttaaatatt tttccagtgc atacaattgc gacttttctg ctaaccctgt ttgatcatga
    11221 aaaactgtaa ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc
    11281 atgttcgcgc acagcatatt tatttacttg gcaaatgatg cctttgcaag tttatgatat
    11341 ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa aaccacccat
    11401 tgacattttt aataatgttt taacagacaa tgatggttct tagcgccgat ttttagcaga
    11461 ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg
    11521 atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg
    11581 ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt
    11641 tgctttaatc accaccagat ggcgttcgcc atccagggca ggaacctgaa gtttaaccac
    11701 tgattcaacc tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt
    11761 cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt
    11821 cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc
    11881 taccctgctc tgtactggct caatattctc cagtttaagc tcatgttgca cctgacgaag
    11941 gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat
    12001 agagagtgga atgccaggca gtccaggacc ggtgccgaca tcgataaacc gttcaccttg
    12061 cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc
    12121 gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat
    12181 aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca gtaaggagag
    12241 tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca
    12301 ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag
    12361 cagaatggag atggccgcag gcgtgacgcc agaaatacgt gaagcctggc cgatagaggc
    12421 cggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta
    12481 atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg
    12541 ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc
    12601 gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg
    12661 acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt
    12721 cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc
    12781 acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc
    12841 cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgca gcatcagacg
    12901 atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc
    12961 atcaaccagt acgccgagat aggcctgaga acgcgccgga gcccaacctt ctttgtcagc
    13021 agacagacgg gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc
    13081 ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt
    13141 cggtttcagg tcgcgtggat cgaagaagtc atactcaatg gcataacccg gacgcacgat
    13201 cttcgcgttt tccatcccct gcatggagcg gacgatttgc atctgcacat cgaacggcag
    13261 gctggtggag ataccgttcg gataaatttc gttagaggtc agcccttccg gttcaaggaa
    13321 gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg acgggcagta
    13381 gcgtgggccg acaccttcga tcacccctgc gtacattggg ctacgatcga ggttactgcg
    13441 gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg
    13501 ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc
    13561 aagcacgcta aagtcgatgg ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg
    13621 accaacgcgc agcggaagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc
    13681 agcacggcca ccgctgtagt tatccagacc gatatgaatt ttaccgtcga ggaacgtccc
    13741 aacggtgagc acaacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac
    13801 cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcagattcgg
    13861 ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg
    13921 ggtagcgcga acagccggtc ctttacttgc gtttagtatc ctaaactgga tacccgcctg
    13981 atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt
    14041 cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt
    14101 caaaagcaga gtctgttgac ccatacgcgc cgcagccatc gcggcctcgg tgcctgcatg
    14161 acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca
    14221 taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgttggct
    14281 tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc
    14341 tattgtgatc tcttattagg atcgcactgc cctgtggata acaaggatcc gacttttaag
    14401 atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc
    14461 tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg ttctttggat
    14521 aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta
    14581 tacttatttg agtaaattaa tccatgatcc cagccattct tctgccggat cttccggaat
    14641 gtcgtgatca agaatgttga tcttcagtgt ttcgcccgtc tgttttgcac cggaattttt
    14701 gagttcagcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat
    14761 accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata
    14821 gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taaccagcca
    14881 gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc
    14941 cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct
    15001 gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt
    15061 acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag
    15121 ggcttgatgg tacgcatgat cgggttctgc aggacgatca gtgtctcggt ggactgaatt
    15181 tcatcaattg tttggatctt gttgataagt acatgctgga gagcatcgat cgaacggcac
    15241 atcactttta taaagatgct gtagtggccg gtggtgtagt acgcctcggt cacttcatcg
    15301 aggctttcca gctttgccag cgcggaagga tagtctttgg cgctctttaa tataatgccg
    15361 ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta
    15421 atgattcccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca
    15481 aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg
    15541 ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc ggattgatga
    15601 ttcattctat tttagccttc ttttttaatg aatcaaaagt gagttaggct ttttattgaa
    15661 tgattattgc atgtgtatcg gtttttgttg cttaatcata agcaacagga cgcaggagta
    15721 taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac
    15781 ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc
    15841 cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa
    15901 gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt
    15961 cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc
    16021 cttcgccccg atgaagaccg tctttctccg ttgcactcgg tctatgttga ccagtgggac
    16081 tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag
    16141 gcgatctggg cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt tggcctggca
    16201 ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg
    16261 gatcttgatg ccaaagggcg tgagcgggcg atagcgaaag atcttggtgc ggtattcctt
    16321 gtcgggattg gcggcaagct gagcgatggt catcgtcacg acgtgcgcgc accggattat
    16381 gatgactgga gcaccccgtc agagctgggc catgcgggcc tgaacggcga tattctggtg
    16441 tggaacccgg tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc
    16501 gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg
    16561 catcaggcgt tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct
    16621 cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg
    16681 ccagctgctg ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc
    16741 tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga
    16801 tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct
    16861 gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgggcgata aaatcagaaa
    16921 tcaccaccgc atcggcatca aaccattcct tgctttgcaa gcgttccata atggcgcgaa
    16981 aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt
    17041 cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat
    17101 aacagcgccg gttttcagcg agagcaatgc gcatcaaagc caggcagaac gctttcgcac
    17161 actgttcatt aaagccgccc attgagccgg aagtatccac gcagacaata aacggtccgc
    17221 gcggctgttc gtcgtaatct ttatgcacca ccgggcgttc gatcactttt tcacgccacg
    17281 actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat
    17341 actccagttc cgttatccct agtgtcgcca gttctggcgg cagaagacgt aaaatatcat
    17401 cgctttgttg cagaccatca acctgctcag gaacagttgc cggttcgcgc accatggtgc
    17461 ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccgg gaacgcccca
    17521 gctgttctgc caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa
    17581 tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga cgaccagctg
    17641 cggtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa
    17701 cttcactcaa cagttgttcg cgttcttctt ctaataactg ttgatttaac gtcgttgctt
    17761 gcacgatcag acttaaacgc caacgctgga ggaaaagcgt gtgtaacgct gaagtgatcg
    17821 tgctgttagc atcaaccaac tgtcgggctt gttctgccca tggggaattc agacgatgca
    17881 gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt
    17941 gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgat
    18001 tacgcagcgc ctcacgccag cggggaacat catcagtgat tgccgccttc agtcgtggga
    18061 atttttcaaa gaagaccgct agctgcggtg aggccagcag cgcgatgatc atctcttcga
    18121 tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg
    18181 tgcctggcga atctgtgcgc cgacatcctg taggctggct tcaatgcgac ctaaccagtc
    18241 gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct tacgccagtc
    18301 gctttcaagc tcttccagtt gttgttttat ttcacctggc agaccttcag ccgatgaacc
    18361 gggaagagcc agcgtactgc cttgtaaact tacatcgcgt acaacaagat gttgggtgct
    18421 atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc
    18481 accaccttta ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc
    18541 catatcatgc agttttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt
    18601 aacagggagt tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa
    18661 ggctgtttta tcactttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgccaag
    18721 gcgagtcaac atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg
    18781 ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc
    18841 taccggggca acagcactgc gaccgctaaa aaaggcgcag gcctgcaata agcggatcgc
    18901 ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg
    18961 cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg
    19021 ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc
    19081 atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca
    19141 cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc
    19201 gttggaggcc gccaccagta gacgcatcgg gattttttct acgtgtgcac cgttgcggaa
    19261 ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat
    19321 ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg
    19381 cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa
    19441 gcgagtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc
    19501 gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc
    19561 cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact
    19621 gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc
    19681 tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata
    19741 gtctcaaagg gggggatttt tctttgagcc aggttaatgt ggccgcattt aggagtacga
    19801 ttttgccgtt aatcgtgcat actgtgcgct tttttgtggg ccaagggact aagcacacat
    19861 ttcatatttc aacgaaagac tagtctatga gcactgataa taagcaatca ttgcccgcga
    19921 ttaccctcgc ggcgattgga gttgtctacg gcgatattgg taccagcccg ttatatacac
    19981 ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga acgcgatgcc gtgtttggct
    20041 ttttatcgct gatcttctgg ctgctaatct ttgtggtttc cattaaatat ctcaccttcg
    20101 tgatgcgggc agataacgcc ggtgaggggg ggatcctgac gttgatgtcg cttgccgggc
    20161 gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat ggggctaatc ggcggcagtt
    20221 ttttctatgg tgaagtcgtc ataacacccg ctatttcggt gatgtcagcc attgaaggtc
    20281 tggaaatcgt cgccccgcag ctggatacct ggatagttcc cctctcaatt atcgttctca
    20341 ctttattatt tatgattcaa aagcatggca cggctatggt cggtaagctg tttgcgccga
    20401 tcatgctgac ctggtttttg attctggcag gactggggtt acgtagcatt attgctaacc
    20461 cggaagtgct gcatgcgctg aatccgatgt gggcggtgca tttcttcctt gaatacaaaa
    20521 cggtttcttt tattgcatta ggggcagtgg tgctgtcgat tacgggggtc gaggcgctgt
    20581 atgctgatat gggacacttt ggtaagttcc ctattcgtct ggcgtggttc actgtcgtat
    20641 taccttcctt aactcttaat tacttcggcc agggagcgct gttgttaaag aacccggaag
    20701 cgattaagaa cccgttcttc cttttggcac cggactgggc gctgatcccg ctgctgatca
    20761 tcgccgcact ggcgacggta atcgcctcgc aggcggttat ctctggcgtc ttttcattga
    20821 cgcgtcaggc ggtacgtctg ggatatttgt cgccgatgcg cattattcac acctccgaaa
    20881 tggagtcagg gcaaatctat attccgtttg tgaactggat gctctatgtc gcggtcgtga
    20941 ttgtgattgt cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga
    21001 ccggaaccat ggtgctgacg tctattctct cgactaccgt ggcacgtcag aactggcact
    21061 ggaataagta ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attccattgt
    21121 tcaccgctaa cctcgataaa ctgctctccg gcggctggtt gccattgagc ctcggtactg
    21181 tgatgtttat cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc
    21241 atgaacatgg taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcctgttc
    21301 gcgtgcccgg gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga
    21361 tgcataacct caaacataac aaggtattgc atgagcgggt gattctgtta actctgcgca
    21421 ccgaagacgc gccatatgtc cataacgtcc gtcgggtaca gattgaacaa ctgtcgccca
    21481 ctttctggcg cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt
    21541 tccaccgctg cggtctggaa ggattaagtt gccggatgat ggaaacctcc ttctttatgt
    21601 cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt
    21661 acttgctgct gcaacgtaat gcgctgcgtg caccagatca atttgaaatc ccgccaaaca
    21721 gggttatcga actgggtact caggtcgaaa tctaacgcca gacgcctcct ttcttcataa
    21781 gggggcgttt ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt
    21841 tccgtaacgt cacgatggtt ttcccaactc agtcaggatt aaactgcggg tcagcgaaac
    21901 gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg
    21961 gtgatctccc gtctgggaca taccgatacg ctggtggtgt gtgatgctgg gttacccatc
    22021 cccaaaagta caacgcgtat cgatatggca ttaacccagg gtgtaccttc ttttatgcag
    22081 gtgctgggcg tcgtcacaaa tgaaatgcag gtcgaggcgg ccattatcgc ggaagagatc
    22141 aaacaacata atccgcaact ccacgaaacg ttgctcactc accttgagca gctgcaaaaa
    22201 caccagggaa ataccattga aattcgttac accacgcatg aacaattcaa acaacaaacc
    22261 gcagaaagtc aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc
    22321 tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga
    22381 taaagccttc ccgggcgtaa aagccctctc gggcgctgcg ttaaatgtct atccgggccg
    22441 cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac
    22501 tggtatctat actcgcgatg ccggcacgct tttatggctg gggaaagaaa cgacatttac
    22561 cgggccgaaa tcttcccagg aagccgggat tgggattatc catcaggaac tgaacctgat
    22621 cccgcagttg accattgccg aaaacatttt cctcggtcgt gagtttgtta atcgctttgg
    22681 caaaattgac tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct
    22741 gcgctttaaa agcgacaagc tggtgggcga tctttccatc ggtgaccagc aaatggttga
    22801 aatcgccaaa gtgctgagct ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc
    22861 gctgaccgat accgaaaccg aatccctgtt ccgcgtcatc cgcgagctga aatcgcaagg
    22921 ccgcggtatt gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt
    22981 taccgttttt cgtgacggac aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga
    23041 ttcgctgatt gagatgatgg tgggtcgcaa gctggaagat caatatccgc acctggacaa
    23101 agcgccggga gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt
    23161 ctcttttact ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgcaggtcg
    23221 taccgaactg atgaaagtgc tctacggcgc actaccgcgc accagcggtt acgtcaccct
    23281 ggatgggcat gaagtcgtta cccgttcacc gcaggatggc ctggcaaacg gcattgtgta
    23341 tatctccgaa gaccgtaaac gtgacggttt agtgttgggc atgtcagtaa aagagaacat
    23401 gtcgctgaca gcgctgcgct acttcagccg cgctggtggc agtttgaagc atgccgatga
    23461 acagcaggct gtgagtgatt tcattcgtct gtttaatgtg aaaactccgt cgatggaaca
    23521 ggcaattggt ctgctttccg gtggcaatca gcaaaaagtg gcgattgccc gcggtctgat
    23581 gacacgcccc aaggtgttga tcctcgatga gcctacccgt ggcgtagatg tcggcgcgaa
    23641 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt
    23701 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
    23761 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
    23821 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
    23881 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
    23941 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
    24001 atattctcca gcaaacttcg gtgaacgcca ttatggcggt tgggatgacg ctggtgatcc
    24061 tgacgtcggg catagacttg tcggtaggtt ctctgttggc gctaaccggc gcagttgctg
    24121 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag
    24181 gtgccgcaat tggtgcggta accggagtga ttgtagcgaa aggtcgcgtc caggcgttta
    24241 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaacggta
    24301 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
    24361 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
    24421 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
    24481 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
    24541 gtctgctggc atcactggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca
    24601 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
    24661 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
    24721 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
    24781 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
    24841 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
    24901 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
    24961 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataaa cttggctata
    25021 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
    25081 taaccgttcg cggcacaaaa attctgctga ttaacccgac cgactccgac gcagtgggta
    25141 atgctgtgaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
    25201 cgaaaggtga agtggtgagc cacattgcat ctgataacgt actgggcggc aaaatcgccg
    25261 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
    25321 ctggtacatc cgcagcccgt gaacgtggcg aaggcttcca gcaggccgta gctgctcaca
    25381 agtttaatgt tcttgccagc cagccagcag attttgatcg caccaaaggt ttgaacgtaa
    25441 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
    25501 tggcgctggg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
    25561 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
    25621 tcgctcagct acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga
    25681 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
    25741 tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
    25801 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
    25861 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
    25921 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
    25981 atttggcggc aaaggcgcga atcaggctgt ggctgctggg cgtagcggtg cgaatatctc
    26041 ctttattgcc tgtactggcg atgacagcat tggcgagagc gttcgccagc agctcgccac
    26101 tgataacatc gatattactc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
    26161 gatttttgtt aatggcgaag gtgagaatgt cattggtatt catgccggcg ctaatgctgc
    26221 cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cagcattatt
    26281 aatgcagctg gagtcaccac tcaaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
    26341 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aactgctggc
    26401 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
    26461 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac
    26521 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
    26581 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
    26641 cgcgttaatc acggcgttgc tggaagaaaa accattgcca gaggcgattc ggtttgccca
    26701 tgctgccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
    26761 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
    26821 ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
    26881 cagtgaagcg attaccgcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
    26941 agctctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
    27001 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
    27061 cggttatagt ctcgtccttt gcaataccga aggcgatgaa cagcggatga atcgcaatct
    27121 ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta ctgtgcaccg aaacgcatca
    27181 gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct accgtgatga tggactgggc
    27241 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
    27301 aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc tgtattaccg gcccgctgga
    27361 taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct
    27421 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
    27481 tatgcgccaa ctgctatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
    27541 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
    27601 cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaaccac
    27661 tatccatcaa ccaaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
    27721 aacccagccg acccttcagc aacaacgatt acaacttact ccgattctga tggaacgcgg
    27781 ttcggcttag atttacgctg tcttttgatc aaattattac catcggttgt tttcagaagc
    27841 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
    27901 tgttcgacag tcgttgtgcc ttccatccct tcataaacgc gaaggacggc cgcacttaca
    27961 gcaacgccta aactgataga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
    28021 gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ctgcgtcgac
    28081 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgccggtgat
    28141 tgcaaagaga actgagcgat cattagccca ataatcaccg tgatccccac taatgtatgg
    28201 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
    28261 gctgtcggtg ccatcataca gccggcaata aacgcctgat aaccaaatcc tacctgtaac
    28321 atcaatggca taaggaacgg tacacagccg gtccccagac gggttgcaat attgcctacg
    28381 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg
    28441 cgtcgtgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat taccgtcaag
    28501 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca
    28561 aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa
    28621 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa
    28681 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
    28741 ggccccacca gacccggcat ggcgacaaaa ttcaatactg gaagaagttc attacgagga
    28801 taagcgcgca gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga
    28861 ataacccgga agacaaccag ctgtggtagc gagttggaaa gtgcgcaggc cagagaaccc
    28921 aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg taccgaagcg atcggctagc
    28981 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
    29041 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
    29101 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata
    29161 ctgcgcttct ttttatcgct cattcaatgt cctgttatcg ggttatcact tatcaggtga
    29221 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc
    29281 gacaatcgcc tgatgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
    29341 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
    29401 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
    29461 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgctttct gttctgcggt
    29521 gccaaccgtt gctgccagta ggcaggcttg cggctccaga cagatacgca taaccagaaa
    29581 gtgatcgatg acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc
    29641 aagaaaattc cagttcgatt gtggcattac ccgggtacca attcgcggtc gcggtaaaac
    29701 catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa
    29761 ttgctcgccc agctcaatct caccgggcaa aatggtgccg ggttcatatt caccttttaa
    29821 gatccgttgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
    29881 ttgtgcgctt aaaggcatta ctaatcttcc tttttctttt tattcctcct tagtatgcca
    29941 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg
    30001 gggcttttgc tgaaaaaatg cgcggtc
//
LOCUS       DAZGCQ010000058        29727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000058 DAZGCQ010000000
VERSION     DAZGCQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.058"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..462)
                     /locus_tag="ACHK07_002841"
     CDS             complement(85..462)
                     /locus_tag="ACHK07_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8103151.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(854..1903)
                     /locus_tag="ACHK07_002842"
     CDS             complement(854..1903)
                     /locus_tag="ACHK07_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8103152.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            2035..3252
                     /locus_tag="ACHK07_002843"
     CDS             2035..3252
                     /locus_tag="ACHK07_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8103153.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMAVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(3256..3813)
                     /gene="dcrB"
                     /locus_tag="ACHK07_002844"
     CDS             complement(3256..3813)
                     /gene="dcrB"
                     /locus_tag="ACHK07_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO8103154.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3886..4551)
                     /locus_tag="ACHK07_002845"
     CDS             complement(3886..4551)
                     /locus_tag="ACHK07_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO8103155.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLTDKSEINAL
                     QAS"
     gene            4772..5017
                     /gene="tusA"
                     /locus_tag="ACHK07_002846"
     CDS             4772..5017
                     /gene="tusA"
                     /locus_tag="ACHK07_002846"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO8103156.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(5119..7317)
                     /gene="zntA"
                     /locus_tag="ACHK07_002847"
     CDS             complement(5119..7317)
                     /gene="zntA"
                     /locus_tag="ACHK07_002847"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO8103157.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(7391..8017)
                     /locus_tag="ACHK07_002848"
     CDS             complement(7391..8017)
                     /locus_tag="ACHK07_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO8103158.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISSFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            8158..8517
                     /locus_tag="ACHK07_002849"
     CDS             8158..8517
                     /locus_tag="ACHK07_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO8103159.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(8520..8789)
                     /locus_tag="ACHK07_002850"
     CDS             complement(8520..8789)
                     /locus_tag="ACHK07_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO8103160.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8779..9375)
                     /gene="rsmD"
                     /locus_tag="ACHK07_002851"
     CDS             complement(8779..9375)
                     /gene="rsmD"
                     /locus_tag="ACHK07_002851"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO8103161.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAKCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLYREKVAGQVAYRLYQREAQGESDAD"
     gene            9525..11021
                     /gene="ftsY"
                     /locus_tag="ACHK07_002852"
     CDS             9525..11021
                     /gene="ftsY"
                     /locus_tag="ACHK07_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO8103162.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKDEM
                     GEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            11024..11692
                     /gene="ftsE"
                     /locus_tag="ACHK07_002853"
     CDS             11024..11692
                     /gene="ftsE"
                     /locus_tag="ACHK07_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO8103163.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            11685..12743
                     /gene="ftsX"
                     /locus_tag="ACHK07_002854"
     CDS             11685..12743
                     /gene="ftsX"
                     /locus_tag="ACHK07_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO8103164.1"
                     /translation="MNKRDAINHIRRFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12988..13842
                     /gene="rpoH"
                     /locus_tag="ACHK07_002855"
     CDS             12988..13842
                     /gene="rpoH"
                     /locus_tag="ACHK07_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO8103165.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            14113..15216
                     /gene="livJ"
                     /locus_tag="ACHK07_002856"
     CDS             14113..15216
                     /gene="livJ"
                     /locus_tag="ACHK07_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO8103166.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(15416..15799)
                     /gene="panM"
                     /locus_tag="ACHK07_002857"
     CDS             complement(15416..15799)
                     /gene="panM"
                     /locus_tag="ACHK07_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO8103167.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            16223..17332
                     /gene="livK"
                     /locus_tag="ACHK07_002858"
     CDS             16223..17332
                     /gene="livK"
                     /locus_tag="ACHK07_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO8103168.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            17380..18306
                     /gene="livH"
                     /locus_tag="ACHK07_002859"
     CDS             17380..18306
                     /gene="livH"
                     /locus_tag="ACHK07_002859"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO8103169.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            18303..19580
                     /gene="livM"
                     /locus_tag="ACHK07_002860"
     CDS             18303..19580
                     /gene="livM"
                     /locus_tag="ACHK07_002860"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO8103170.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            19577..20344
                     /gene="livG"
                     /locus_tag="ACHK07_002861"
     CDS             19577..20344
                     /gene="livG"
                     /locus_tag="ACHK07_002861"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO8103171.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            20346..21059
                     /gene="livF"
                     /locus_tag="ACHK07_002862"
     CDS             20346..21059
                     /gene="livF"
                     /locus_tag="ACHK07_002862"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO8103172.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            21178..21924
                     /locus_tag="ACHK07_002863"
     CDS             21178..21924
                     /locus_tag="ACHK07_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8103173.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQNKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            22284..23600
                     /gene="ugpB"
                     /locus_tag="ACHK07_002864"
     CDS             22284..23600
                     /gene="ugpB"
                     /locus_tag="ACHK07_002864"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO8103174.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            23698..24585
                     /gene="ugpA"
                     /locus_tag="ACHK07_002865"
     CDS             23698..24585
                     /gene="ugpA"
                     /locus_tag="ACHK07_002865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO8103175.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            24582..25427
                     /gene="ugpE"
                     /locus_tag="ACHK07_002866"
     CDS             24582..25427
                     /gene="ugpE"
                     /locus_tag="ACHK07_002866"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO8103176.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            25429..26499
                     /gene="ugpC"
                     /locus_tag="ACHK07_002867"
     CDS             25429..26499
                     /gene="ugpC"
                     /locus_tag="ACHK07_002867"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO8103177.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            26496..27239
                     /gene="ugpQ"
                     /locus_tag="ACHK07_002868"
     CDS             26496..27239
                     /gene="ugpQ"
                     /locus_tag="ACHK07_002868"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8103178.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(27226..27666)
                     /locus_tag="ACHK07_002869"
     CDS             complement(27226..27666)
                     /locus_tag="ACHK07_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO8103179.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            27786..29531
                     /gene="ggt"
                     /locus_tag="ACHK07_002870"
     CDS             27786..29531
                     /gene="ggt"
                     /locus_tag="ACHK07_002870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO8103180.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         6966 a         7540 c         8218 g         7003 t
ORIGIN      
        1 aatcctgttc gtacatgttg ggcacaacct gttaagaggg aattaaatta ttatcggacc
       61 gcaacggccc gactttagga agaattataa atctgcgccc gctgacaaat tcagttgaat
      121 atctttgtcg agattgcgga cccagcgatt gtagttttta tgaatttgtc cgcctgacgc
      181 ttgtaggttc tggctatttt cataattgat atcgtagctg gatgccgtgt agttaatgcg
      241 tatttccgct gaatgatcgc gtgactgttg acgtgcttta attatgcctt gtccggcttc
      301 ggacataatc catttgcgtt caacgccagc tttcagaatc gcttttttca cttgttcctg
      361 ggtatgacca gcgctgacgg ttgagtgaac ctgatcaatt ggcgcagtac gcgcgcaacc
      421 tgctaaagca cccacaacag caatagccgc aaaccatttc atgtaatgtc tcattagaac
      481 cccattaaag tattgttttt acgagtaaag caagatttaa ctgtaattgc tggcatcact
      541 attctttatt tagagaaaaa attacttgtg agataagtca gtaagtgcac atcagagtaa
      601 aaatctctga aatgactaaa ttactgccag cagggaccaa ctataagtga gtgagaaaaa
      661 tgaggcaata aaaataacac aatgttatca gtgcattctt tgagaaggat ttaatcgcta
      721 ttcggcgttt atatatattt gacttaagga atggtttttt taaattaatt ttctgaataa
      781 tgtttcatca tttcctcaaa tcaaatggcc actgttagat aaacggtggc cattgcctgc
      841 tcaggaggtg ctattaaccc ggtaaccgac ttttcggtct gcccggcccc agtaaaatcg
      901 ccagtttgct gccgcctttc gtcgtttcca tccagatttt acacacgctg gttaatggca
      961 ccgaaagtag catccctacc gggccgagca gccatcccca aattaacaat gaaagaaata
     1021 ccaccatggt ggacatcccc aggcgatggc ccatcatccg tggttctaaa atattgccga
     1081 tgaccatatg gaccactaaa aacaatgcgc cgaccagaat acattcgtaa acaccattaa
     1141 acagcagcac ctgaatcatt ggcggtacgg cggaaattac cgcgccgata ttgggcacgt
     1201 agttaagcaa aaacgccagt actgcccaca tcagcgcaaa ctgcaccccc atcagctcca
     1261 gccccagcca gacgatgaca cctgtccata aactgagtag cgtctttaac gccagataat
     1321 gcgaaacgcc tttaagtgcg cggtgtaatc ctgcgatgtg aatctgtggg ttattcaatg
     1381 caaaacgcat tttgtaaggg acgtggcgca cttcaaacag cataaaaact acggtcatca
     1441 ccagcaaaag cacgctcgcc attgccccgg aaagcccggt cattagcgct gtggtgaagg
     1501 taaccacttt ttccgagtcc atccgctgca gcattcgctc cggcgacata tgcaaattaa
     1561 gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg
     1621 gcagcataga gataaattcg ttaaacgatg ccgccagtac gccgaccagc gcggttagtg
     1681 tgatcagcat caccaccacc acaatcgtaa tggcaacggg gcgttttact ccccgtcgaa
     1741 taaaccaggt aacgagcggg ttgaggacga tggcaaaaaa cagcgccagt aacagctgca
     1801 caatgatatc tgccgctgcg tgaatgcccg cgaggatcac taccagcgag gccagcttga
     1861 gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc cattggggct tccttgtgac
     1921 tttttgtatt aagtgtagcg ggagtcacgc cagcaatatt ctgattctta ctgctgattt
     1981 tcacgtcagc acgtggtaaa aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc
     2041 gaacccgtag ccgaacccgc gctaaacgga ttgcgcctga atttgcgcat tgtctccatt
     2101 gtcatgttta acttcgccag ctacctcacc atcgggttgc cgctcgctgt attaccgggc
     2161 tatgtccatg atgtgatggg ctttagcgct ttctgggcag gattggttat cagcctgcaa
     2221 tattttgcca ccttgctgag ccgtcctcat gccggacgtt acgccgattt gctgggcccc
     2281 aaaaagattg tcgtcttcgg tttatgcggc tgctttttga gcggtctggg atatctgacg
     2341 gcaggattaa ccgccagtct gcccgtcatc agcctgttat tactttgcct gggacgcgtc
     2401 atccttggga ttgggcaaag ttttgccgga acgggatcga ccctgtgggg cgttggcgtg
     2461 gttggctcgc tgcatatcgg acgggtgatt tcgtggaacg gtattgtcac ttatggggcg
     2521 atggcgatgg gtgcgccgtt aggtgtcgtg ttttatcact ggggcggctt gcaggcgtta
     2581 gcgttaatca ttatggccgt ggcgctggtg gccattttgt tggcgatccc gcgtccgacg
     2641 gtaaaagcca gtaaaggcaa accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg
     2701 tacggtatgg cgctggcgtt ggcttccgcc ggatttggcg tcatcgccac ctttatcacg
     2761 ctgttttatg acgctaaagg ttgggacggt gcggctttcg cgctgacgct gtttagctgt
     2821 gcgtttgtcg gtacgcgttt gttattccct aacggcatta accgtatcgg cggcttaaac
     2881 gtagcgatga tttgctttag cgttgagata atcggcctgc tactggttgg cgtggcgact
     2941 atgccgtgga tggcgaaaat cggcgtctta ctggcggggg ccgggttttc gctggtgttc
     3001 ccggcattgg gtgtagtggc ggtaaaagcg gttccgcagc aaaatcaggg ggcggcgctg
     3061 gcaacttaca ccgtatttat ggatttatcg cttggcgtga ctggaccact ggctgggctg
     3121 gtgatgagct gggcgggcgt accggtgatt tatctggcgg cggcgggact ggtcgcaatc
     3181 gcgttattac tgacgtggcg attaaaaaaa cggcctccgg aacacgtccc tgaggccgcc
     3241 tcatcatctt aaaacttact gaataaccag cgtattaatg atgttttctg cggtggtctg
     3301 cgctttttgc tgatcgtcag ctggcagagt aatttgcatg gtcagcagtt gattacccac
     3361 gttacccaga ataacggaag agtacgccgt ctggcctttc gcggagataa tgctgtctaa
     3421 ctgctgcatt ttgtgacctt tcagctcaat ggctttgttg gtcactactt gcagctgcgg
     3481 atcgcggcta cgttgctgat cttccagacg cttcgccagc accgccagat cttctttcgg
     3541 atcatcgccc atgatgacga tgactgcttt ctgcccggtg gcgtcggacc agacatgcat
     3601 gttattggcc tgcgttccca gcttaccgct ctggtcggtc atatccgctg gcagcgagaa
     3661 acttaacttg ccatcaagca ggttgacggg attcccggta gcgttacttt ccgcgaccga
     3721 accctgcgcc gtagcgttag tgtctttatc atcacaggcc gcaagcccca taaccagcag
     3781 gccaattccg acatatttaa ccagattgcg cattgacttc ttcctttcga taaacggcca
     3841 taacggctca ttcatccatc ttatcacaac tctgataacg aacctttaac tcgcctgcaa
     3901 agcgttgatt tcggatttat ctgtcagtct tttcaacagc atattgagta atacgccata
     3961 cattggcagg aagaaaacga tactgattaa cactttgaaa cagtaatcga ccagcgcgat
     4021 ttccatccag tgttcagcca taaaggcatc cgggctacgc cagaaggcaa tgaagaaaaa
     4081 ggccagcgtg tcgctgacgt taccgaacag tgtggacgct gtcggtgcca gccaccagcg
     4141 gcgactctga cgcaggcggt taaaaacgtg cacgtcgagg atctgtccca gcgcgtaggc
     4201 catgaaactg gcggtggcga tacgggcgac aaacaggttg aagtgggcga gtgcgccgaa
     4261 tccctgccag gaacccatat agaatagcga cgagatgacg taggagatca gcaatgccgg
     4321 gatcatcacc gcaaagatga tacgacgggc cagcggtgcg ccaaaaatac gtacggtcag
     4381 gtcggtagca aggaaaataa acgggaagct aaacgcgccc caggtggtat ggaaaccaaa
     4441 aatggagacg ggaagctgaa ccagatagtt actggaggtg atcaccagca gatgaaataa
     4501 cgataaccag aacaacgcct tatagcgttg agtttgcgag aaaacgttca tattgtacct
     4561 ttttgattaa ccattggggt gagggaaccc aatacgtacg acacgtctgt taccttaaca
     4621 taataacgtg cttacccttt tttcgagccg ccgcatgata ctgctttacg ttgacaatgc
     4681 aatggttaat tttcacgcaa tcgttaacct ggtttgctta cggactcaca gggcgtaaac
     4741 tagcgccgtt tttttatgtg atgagaagaa aatgaccgat ctcttttcca gccctgacca
     4801 cacactcgac gcacttggcc tgcgctgccc ggaacccgta atgatggtgc gcaaaacagt
     4861 gcgcaatatg cagcctggtg aaacgttgct gattattgct gacgatccgg cgacgacccg
     4921 cgatattcct gggttttgta cctttatgga acacgaactg gttgctaaag agacggatgg
     4981 actgccttat cgttatttga ttcgtaaagg cggttgatgg gggcagattg gcttcgatgc
     5041 cgccttttcc cctcacccta accctctccc cagaggagcg aggggaccga tcgcgctcaa
     5101 tgttgcgatc ggtttgcctt atctcctgcg caacaatctt aacgcattcg ctgtcaccag
     5161 caccgtcgca cccgtatctg ccagcaccgc cagccatagt ccggtcattc ctaacagcgt
     5221 ggtgacgagg aagatccctt tcagccccag cgcaatagtg atgttctggc ggatattggc
     5281 gtgagtggcg cgtgccagtt caatcatttg caccagaccg cgcaggtggt tatgggttaa
     5341 tgctgcgtcg gcggtttcca gcgccacgtc tgtgccgctg cccattgcaa tcccgatggc
     5401 ggcagctttc atcgctggcg cgtcgttaat accgtcaccg accatcgcca gcggcgcatg
     5461 ttgattcagc tcggttaccg ctttgacttt atcttccggc aacaggcccg ctttaaactc
     5521 cagccccagc tccccggcaa ttgccgccgc tgcgcgtgga ttatcgccgg tgaggatcac
     5581 ccctttgacg cccagcgcgt tcagttcact gatggcagtt gcagcatcgg cgcgcagggt
     5641 atcctgtaac gcaatgacac ccagcacgtc atcgttacgt actaccagca ctaccgtttg
     5701 cccggcgctt tccagttcgt taattaaacc agcaaatgca tcagcgggat gtttcccggc
     5761 agcgcaaatc aatacgcgct caccgttaac ctgcgcttca atgccagacc cgaccagcgc
     5821 ccgctgtgat tcggcggcgg gaatggcgag tgcagcaacc tgtgcttcgc gcacgatggc
     5881 ctgcgccagt ggatgcgtcg cgccttgctc gaccgccgcc gccagtgtca gcagttcaga
     5941 ttcactaata cccgttgccg gatgaatcgc ggtaacgcgc ggtttaccga cggtcagcgt
     6001 accggtttta tcaaacgcca cctgggtaac acgacctagc tgctccagcg ccgcaccgcc
     6061 tttaattaac gccccacgac gtgccgctgc tgccagcccg gaggtaatcg ccgcgggcgt
     6121 ggagataact aacgcacacg ggcagccaat cagcagcagc gtcaggcctt tataaatcca
     6181 ctcttgccag ctggcggcaa acagcagcgg cggcaccagc gttaccagca gggcgacggc
     6241 cataatcgct ggcgtataaa tacggctgaa acggtcgata aaccgctcaa tgggagcgcg
     6301 acgctcttcg gcttcttcga tcagtttcag aatccggtca atggcgctgg ctcccggttc
     6361 ggacaatact tccagcgtta ccagacggtc tacgctggtg gctcctgcag gaactttatc
     6421 gcccgttgcg cgctccaccg gaatggattc gccggtcagg gcgctttcat caaaactggc
     6481 aaagggcgag agcagtttac cgtcggcagg caaacgcccg cccgccgcaa cttcaatcac
     6541 atcgccaggg cgcaggctgt taatcgccac ctcttcccgc tcaccgttac gcaggcgcgt
     6601 ggcggtttct ggtttcagcg ccatcaacgc gctaacgccc tgacgcgcgc ggctggcggc
     6661 ccagccttcc agtcgttcac caatcaaaaa cagcagcaac accatcgcag cttcagccgt
     6721 tgcgccaata aacagcgcac caatagcagc tacgctcatt aacgtttcaa tggcgaagta
     6781 actgcctgat ttgatcagcc gtaatgcctg acgagcaatc gggtacagcc caaccagcgt
     6841 ggtcgcgata aacgccagtt gaccgaacgg atgattaaat tgctccagtc cccagctgat
     6901 tgccatcatc acgataagcg taatcagcgg cagattctct ttcaggcgcg attcttgtgg
     6961 ctcgtcggcg gcctgctcat cgcgcaggga atagcccgct ttttgcaccg cagattcaac
     7021 ctgggcacgg atgtcattgt cggcatcaac caccagtttt tcggtggcga acagtacctg
     7081 cacctgattc acgcctgcaa gctggcgcac ggcattttct accttgcgcg cacaggcggc
     7141 gcagtccatg ccgctgactt tccagctata gcgggtgccg gagacgtttt cagagagcgt
     7201 tggcgtgctg gaacatgcgc cgtcgcagca acagtcgttg gcgttctgta ccgtggttag
     7261 cggtttgaac gcagcaaatt gaggggcttt cttgccgtga ttgtcaggag tcgacatggc
     7321 atcctccggt taagtttttt ctcattaacc gaaggataca ctctggagtc gactccagag
     7381 tcaagtttta tcagagatac agcgagcgga cgatgaggaa gtgaccggca aagtagcagg
     7441 ccgcagcaat cgcgttatcc gcacggaagc gtcggcgata gtggctaccc agccagacaa
     7501 agctactgat aaacagcaac gaagcgccga caaacgcaga gagtgccgga gcggtcggac
     7561 ggaagaacca cagttcacct gccagccaca ccatcaccag cgtcatgccg ataaacgtgc
     7621 agataggcca acgatactct tccagccgcg tccagataat cgccagtaac agcgcgccca
     7681 gaaccagcag taccaacggc agaggccaga agaaagagag cgtcatctga ctggcgaaat
     7741 agatggtgta cagcaggtgc gagaggaaaa acgcgccgat ggcgtacatc agacgttgac
     7801 gtggcaacag ggttagcgca tcgcccagca gtgaggcgca cagccctgcc agcaccagat
     7861 agctaatggc gtcgaacatc ggcgcttgcc aggccagtaa cagcaggaga agaagggtta
     7921 acggtttaaa cacccagcgt tgccaggttg gcccacgata cgatgcatcc acagatagcc
     7981 atgcggaaag acagacagcg ataaacgacc aaagcatctt agttccttga attcgttatt
     8041 ttttcgtagg cggaatgcct acggtcttgc ttccagttta gtggcgaggg tggacggatg
     8101 acaacaccat aactggcagg gtatgcttaa ttccgcattt tccgatgagg gataaagatg
     8161 agcaaaccac ctcttttctt tattgttatc attggcttaa tcatcgtcgc cgcatcgttt
     8221 cgttttatgc aacagcgacg ggaaaaagct gataatgata tggcaccgct ccagcaaaag
     8281 ctggtggtgg tgagcaacaa gcgggaaaaa ccgattaacg atcgccgttc gcgtcagcag
     8341 gaagtgactc cggcaggcac cagtatgcgc tatgaggcaa gcttcaaacc gcaaagcgga
     8401 ggaatggagc agacgtttcg cctcgacgcc cagcagtacc acgccctgac agtgggcgat
     8461 aaaggtacgc tgagctataa aggaacgcgc tttgttagct ttgttggcga acaataaggt
     8521 tattttttaa ctttaaattt cttctgccag accagcagtt caaacacgcc gaaaaggaaa
     8581 attcgcacct tttcggcggt ggtcatctgc gggccatctt tcggtaaagt ggatttcaat
     8641 agcgccagct gcataccatg catcagcacg gtaaaaatca gcgccacgtt aacgaaaata
     8701 ttcagcgggc gcgggaaggg atgcaccagg ttgaggatta aaaatcccca aacgcagagc
     8761 attaacaaac gtccaatatt aatcagcatc actttctcct tgtgcttcgc gttgatacag
     8821 ccgataagcc acctgacccg ccactttttc ccgatacaat gaccagtttg ccggaacagt
     8881 gggcagaccg ttttcgactt cgctttcgac ataaatcagg gcttcgtcag ccagccagcc
     8941 gttatcttcc agtaaattta tcgtctcttc taacaagcca cggcggaacg gtggatcgac
     9001 aaacacgata ttatgcggtg tgcctttttg cgccaggaat gacatcgcgt tgctgttcac
     9061 tacgcgtgca ttgcctgctt ttagtgtcgc cagattctta attaactgct gagaaactgc
     9121 acgatccatc tcaatcaacg tggcccccgc agcgtagcgt gataacgctt ccagccccag
     9181 cgcgccgctt ccggcgaagc aatccagaca tttggcgtca acaatgaccg gagccagcca
     9241 gttaaataac gtttcgcgta cgcggtcggt ggtggggcgc agacctgggc tatcaggaac
     9301 cgggagttta cggcctcgcc actgcccgcc aataatgcgg atttggccgc tgccggaatg
     9361 attcggtttt ttcatgataa attgctcaat ccgccagata acacatactt gcaggcggtg
     9421 atgtgaatta agttaagtga tagactattt catcattttt ttagctgcta tgtacatagc
     9481 gttaacgctg tgccatgaag caacagcgag gagtgtagtc gcaaatggcg aaagaaaaaa
     9541 aacgtggctt tttttcctgg ctgggctttg gtcaaaaaga gcagaccccg gaaaaagaga
     9601 cagaagttca gaatgaacaa ccggttgtag aagaaatcgt tcaggcgcaa gagcctgtga
     9661 aggcttcgga acaagccgtt gaagagcagc cgcaggcgca tactgaagcc gaggcggaaa
     9721 cttttgctgc cgacgttgtg gaagtcactg aacaggtagc tgaaagtgaa aaagcgcagc
     9781 ctgaagcgga agtcgttgca cagccggaac cggtcgtaga agaaacgccg gagccagtgg
     9841 ctatcgaacg tgaagaactg ccgttgccgg aagacgtcaa cgccgaagag gtttcgccag
     9901 aagagtggca ggctgaagcg gaaaccgtag agattgtcga agcggcggaa gaagaagcgg
     9961 ctaaagaaga aattaccgac gaagagctgg aagcacaggc gctggctgcc gaagcggcag
    10021 aagaggcggt gatggtggtt cctccggcag aagaagagca gccggtggaa gaaatcgctc
    10081 aggagcagga aaaaccgacc aaagaaggtt ttttcgcgcg cctgaaacgc agcctgttaa
    10141 aaaccaaaga aaatctcggt tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg
    10201 atgatctgtt tgaggagctg gaagagcagc ttttgatcgc cgatgtgggt gtggaaacca
    10261 cacgtaaaat tatcaccaat ctgacggaag gcgcatcccg caagcagctt cgtgatgccg
    10321 aggcgctcta tggcctgctg aaagacgaga tgggcgagat tctggcgaaa gtcgatgagc
    10381 cgctgaatgt tgaaggcaaa gcgccgtttg tgatcctgat ggtgggcgtc aacggtgtgg
    10441 gtaaaaccac gacgattggt aagctggcac gtcagtttga gcagcagggt aaatcggtga
    10501 tgctggcggc gggcgatact ttccgtgcag cagcggttga acagcttcag gtctggggtc
    10561 agcgcaacaa tattccggtg attgcccagc ataccggtgc ggattccgcc tctgttatct
    10621 tcgacgccat tcaggcagct aaagcgcgta atatcgacgt cctgattgcc gataccgccg
    10681 gacgtctgca gaacaaatcg catctgatgg aagagttgaa gaaaatcgtc cgcgtgatga
    10741 agaaactcga cgttgaagcg ccgcatgaag ttatgctgac tattgatgcc agcaccgggc
    10801 agaacgcggt aagccaggcc aaactgttcc acgaagctgt tggcttaacc ggcatcacgc
    10861 taacgaaact ggacggcacg gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg
    10921 gtatccctat ccgctacatt ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg
    10981 cggacgactt tatagaggca ctttttgccc gagaggatta acaatgattc gctttgaaca
    11041 tgtcagcaag gcttatctcg gtgggagaca ggcgctgcag ggcgttacgt tccatatgca
    11101 gccgggtgag atggcgtttc tgaccggtca ttccggcgca gggaaaagta ccctcctgaa
    11161 gctgatctgt gggattgagc ggcccagcgc cgggaaaatc tggtttagcg gccatgacat
    11221 cacgcgtctg aaaaaccgtg aagttccgtt tctgcgccgc cagattggca tgattttcca
    11281 ggatcaccat ctactgatgg accgtactgt ctacgataac gtggcgatcc cgctgattat
    11341 cgccggtgcc agcggtgacg atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg
    11401 gctactggac aaagcgaaga acttccctat tcagctttct ggcggtgaac aacagcgtgt
    11461 tggcattgcc cgcgcggtgg tgaataagcc cgcggtactg ctggcggacg aaccgactgg
    11521 taacctggac gacgcgttgt cggaagggat tctacgtctg tttgaagagt ttaaccgcgt
    11581 tggggtaacc gtattgatgg caacgcacga catcaacctg atttcgcggc gttcctatcg
    11641 catgctcacc ctgagcgatg gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg
    11701 caatcaatca tattcggcgg tttggcgggc gtctggatcg cttccgtaaa tcggtcggcg
    11761 gctcaggcga cggcggtcgt aacgcgccga aacgcgcgaa atcctcgcca aaaccggtaa
    11821 atcgcaaaac caacgttttc aacgaacagg tgcgctatgc cttccacggc gcattgcagg
    11881 atctgaaaag caaaccgttc gccacgtttt taacggtgat ggttatcgcc atctctctga
    11941 cgctgcccag cgtctgttat atggtgtaca aaaacgttaa ccaggcggcg acgcaatatt
    12001 atccgtcacc gcaaatcact gtttatctgc aaaaaacgct ggacgatgac gctgctgcgg
    12061 gcgtggtggc acagttgcag gccgagcagg gcgtggagaa agtgaattat ctttctcgtg
    12121 aagacgcact gggcgagttc cgtaactggt ctggttttgg tggtgcactg gatatgctgg
    12181 aagaaaaccc gcttccggca gtggcggtgg tgatcccgaa actcgatttt caggggacgg
    12241 aatcactcaa tacgctgcgt gatcgtatca cgcagattaa cggcattgac gaagtgcgga
    12301 tggatgacag ctggtttgcc cgtctggcgg cgttgaccgg gctggtcggg cgcgtttcgg
    12361 cgatgatcgg cgtgttgatg gtggcggcag tgttcctcgt catcggtaac agtgtacgcc
    12421 tgagtatctt tgctcgccgt gactccatca acgtgcagaa actgattggt gcgacggatg
    12481 gtttcatcct gcgtccgttc ctgtacggtg gcgcactgct gggattttct ggcgcattgt
    12541 tgtcattaat tttgtcagaa attctggtgc tgcgattgtc atcggcggtt gcggaagtgg
    12601 cacaggtttt cggaacgaag tttgatatca atggtttatc gttcgatgaa tgcctgctgt
    12661 tgctgctggt atgctcgatg attggctggg tggcagcgtg gcttgccacg gtacaacatt
    12721 tacgccactt tacgcctgaa taataaaagc gtgttatact ctttccctgc aatgggttcc
    12781 gtagcaggga aagagacccc gttgtctctt cccggtattt catctctatg tcacattttg
    12841 tgcgtaattt attcacaagc ttgcattgaa cttgtggata aaatcacggt ctgataaaac
    12901 agtgaatgat aacctcgttg ctcttaagct ctggcacagt tgttgctacc actgaagcgc
    12961 cagaagatat cgattgagag gatttgaatg actgacaaaa tgcaaagttt agctttagcc
    13021 ccagttggca acctggattc ctacatccgg gcagctaacg cgtggccgat gttgtcggct
    13081 gacgaggagc gggcgctggc tgaaaagctg cattaccatg gcgatctgga agcagctaaa
    13141 acgctgatcc tgtctcacct gcggtttgtt gttcatattg ctcgtaatta tgcgggctat
    13201 ggcctgccac aggcggattt gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc
    13261 cgttttaacc cggaagtggg tgtgcgcctg gtctccttcg ccgttcactg gatcaaagca
    13321 gagatccacg aatacgttct gcgtaactgg cgtatcgtca aagttgcgac caccaaagcg
    13381 cagcgcaaac tgttcttcaa cctgcgtaaa accaagcagc gtttgggctg gtttaaccag
    13441 gatgaagtcg aaatggtggc ccgtgaactg ggcgtaacca gcaaagacgt acgtgagatg
    13501 gaatcacgta tggcggcaca ggacatgacc tttgacctgt cttccgacga cgattccgac
    13561 agccaaccga tggcaccggt gctctatctg caggataaat catctaactt tgccgacggc
    13621 atcgaagatg ataactggga agagcaggcg gcaaaccgtc tgaccgacgc gatgcagggt
    13681 ctggacgaac gcagccagga catcatccgt gcgcgctggc tggacgaaga caacaagtcc
    13741 acgttgcagg aactggctga ccgttacggt gtttccgctg aacgtgtacg ccagctggaa
    13801 aagaacgcga tgaaaaaact gcgcgctgct atagaagcgt aatttccgct attaagcaga
    13861 gaaccctgga tgcgagtccg gggtttttgt tttttgggcc tcaacaataa tcaatttccc
    13921 ctccgacaaa acgccaatcc ccacgcagat tgttaataaa ctgtcaaaat agctattcca
    13981 atatcataaa aatcgggtat gttttagcag agtatgctgc taaagcacgg gtagtcatgc
    14041 ataaaacgaa ataaagtgct gaacaacaac atcacaacac acgtaataac cagaagaatg
    14101 gggattctca ggatgaacat aaagggtaaa gcgttactgg caggatgtat cgcgctggca
    14161 ttcagcaata tggctctggc agaagatatt aaagtcgcgg tcgtgggcgc aatgtccggt
    14221 ccggttgcgc agtacggtga ccaggagttt accggcgcag agcaggcggt tgcggatatc
    14281 aacgctaaag gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt
    14341 gacccgaaac aggcggttgc ggtggcgaac aaagtcgtta acgacggcat taaatatgtg
    14401 attggtcacc tctgttcttc atcaacgcag cctgcgtctg acatctatga agacgaaggc
    14461 attttaatga tcaccccagc ggcaaccgcg ccggagctga ccgcccgtgg ctatcagctg
    14521 atcctgcgca ccaccggcct ggactccgac caggggccga cggcggcgaa atatattctt
    14581 gagaaagtga aaccgcagcg tattgctatc gttcacgaca aacagcaata cggcgaaggt
    14641 ctggcgcgtg cggtgcagga cggcctgaag aaaggcaatg ctaacgtggt gttctttgat
    14701 ggtatcaccg ccggggaaaa agatttctca acgctggtag cgcgtctgaa gaaagagaat
    14761 atcgacttcg tttactacgg cggttatcac ccggaaatgg ggcaaatcct gcgtcaggca
    14821 cgcgcggcag ggttgaaaac tcagtttatg gggccggaag gcgtggctaa cgtctcgctg
    14881 tctaacattg cgggcgaatc agcggaaggg ctgctggtga ccaagccgaa gaactacgat
    14941 caggttccgg cgaacaaacc cattgttgat gcgatcaaag cgaaaaaaca ggacccaagc
    15001 ggcgcattcg tttggaccac ctacgccgcg ctgcaatctt tgcaggcggg cctgaatcag
    15061 tctgacgatc cggctgagat cgccaaatac ctgaaagcga actcggttga taccgtgatg
    15121 ggtccgctga cgtgggatga gaaaggtgat ctgaaaggct ttgagttcgg tgtgtttgac
    15181 tggcacgcca acggcacagc gaccgatgcg aagtaattgt taatcggcaa ctttgggttg
    15241 ccgccaaatt gctaatatag agtacgttgc ttcatgccgg atgcggcgta aacgccttat
    15301 ccggcctaca agatccaaag aaatcagtaa attgcaacac acattgtagg cctgataagc
    15361 gtagcgcatc aggcaatttc tcgttggtca tcacttttga aatcaaactt gacgattaac
    15421 gcttctccca gccgccctgc tgtgccgtaa atcccagtgc ctgcataaac gccgtcatca
    15481 caccgcgatc ttccacgcct gcatccgcca tccaccagca tgaaacgcca ggattgttac
    15541 gcaaaacctc ttccagcaga tattgcccca caccgcgacg tcgagtgact tcccgcacgc
    15601 gcagggaatc cagtgccccc tcggtgccgc ttaaggttac ccgcacggcc gcaagcaggc
    15661 gctcgttaaa acgcgcggcg tagatgcggt ggttatcgtc aacctgtaaa gaggaagggg
    15721 aatactccgg ccagatcttt tgcaggtcaa tccggtcctg gtcgctaaat ttttctaatc
    15781 gaatgatggt cagcttcatg ggtaacccgt gtaaatcaca aaagtggaac cagtgtagcg
    15841 aaataattta atcggaggct ttctcttttt tatttctttt ggcaggtgat taatttttta
    15901 acagcaataa ttacaaagtt aaaacattag agaatgaaaa atgtccagca taatcccctg
    15961 aatgatagtg aattattccg ccctttgtgc cgttatttta tgctgacaaa ggcacttttt
    16021 tctgtttatc tatcaataaa ttcagaatat tatctgttct taatcgactg aaaaataggg
    16081 attttaatcg ctatcatcac aaaatactgc gctaacccct taatcagaca ggcaaaaaca
    16141 gtgcagtata aaaaaagaac agtctgattt gttaacacat aaaaacaaag caacacaaca
    16201 tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa actatcatcg cagggatgat
    16261 tgcactggca atttcacaca ccgctatggc tgacgatatt aaagtcgccg ttgtcggcgc
    16321 gatgtccggc ccgattgccc agtggggcga tatggaattt aacggcgcgc gtcaggcgat
    16381 taaagacatt aatgccaaag gggggattaa aggcgataag ctggttggcg tggaatatga
    16441 cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac aaaatcgtta acgacggcat
    16501 taaatacgtt attggtcatc tgtgttcttc ttctactcaa cctgcatcag atatctatga
    16561 agacgaaggt attctgatga tctcgccggg agcgaccaac ccggagctga cccaacgcgg
    16621 ttatcaacac attatgcgta ctgccgggct ggactcttcc caggggccaa cggcggcaaa
    16681 atacattctt gagacggtga agccccagcg catcgccatc attcacgaca aacaacagta
    16741 tggcgaaggg ctggcgcgtt cggtgcagga cgggctgaaa gcggctaacg ccaacgtcgt
    16801 cttcttcgac ggcattaccg ccggcgagaa agatttctcc gcgctgatcg cacgcctgaa
    16861 aaaagaaaac atcgacttcg tttactacgg cggttactac ccggaaatgg ggcagatgct
    16921 gcgccaggcc cgttccgttg gcctgaaaac tcagtttatg gggccggaag gtgtgggtaa
    16981 cgcatcattg tcgaatattg ccggtgatgc tgccgaaggc atgttggtca ctatgccaaa
    17041 acgctatgac caggatccgg caaaccaggg catcgttgat gcgctgaaag cagacaagaa
    17101 agatccgtcc gggccttatg tctggatcac ctacgcggcg gtgcaatctc tggcaactgc
    17161 ccttgagcgt accggcagcg atgagccgct ggcgctggtg aaagatttaa aagctaacgg
    17221 tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa ggcgatctta agggatttga
    17281 ttttggtgtc ttccagtggc acgccgatgg ttcatccacg gcagccaagt gatcatccca
    17341 ccgcccgtga aatgcgggcg ggtttagaaa ggttacctta tgtctgagca gtttttgtat
    17401 ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta cctacgcgct gatagccatc
    17461 ggctacacca tggtttacgg cattatcggc atgatcaact tcgcccacgg cgaggtttac
    17521 atgatcggca gctacgtctc atttatgatc atcgccgcac tgatgatgat gggcattgat
    17581 accggctggc tgctggtggc ggcgggattc gtcggcgcaa tcgtcattgc cagcgcctac
    17641 ggctggagta tcgaacgggt ggcctatcgc ccggtgcgta actccaagcg cctgattgca
    17701 ctcatctcgg caatcggtat gtccatcttc ctgcaaaact acgtcagcct gaccgaaggt
    17761 tcgcgcgacg tggcgctgcc gagcctgttt aacggtcagt gggtggtggg ccatagcgaa
    17821 aacttctctg cctctattac caccatgcag gcggtgatct ggattgtcac cttcctcgcc
    17881 atgctggcgc tgacgatttt cattcgttat tcccgtatgg ggcgcgcctg ccgtgcctgc
    17941 gccgaagatc tgaaaatggc gagtctgctt ggcattaaca ccgaccgggt gattgcgctg
    18001 acctttgtga ttggcgcggc gatggcggcg gtggcgggtg tgctgctcgg tcagttctac
    18061 ggcgtcatta acccctatat cggctttatg gccgggatga aagcctttac cgctgcggtg
    18121 ctcggtggga ttggcagcat tccgggagcg atgattggcg ggctgattct ggggattgcg
    18181 gaggcgctct cttctgccta tctgagtacg gaatataaag atgtggtctc attcgccctg
    18241 ctgattctgg tgctgctggt gatgccgacc ggtattctgg gtcgcccgga ggtagagaaa
    18301 gtatgaaacc gatgcatatt gcaatggcgt tgctctctgc cgcgatgttt tttgtcctgg
    18361 cgggcgtctt tatgggcgta caactggagc tggatggcac caaactggtg gtcgacacgg
    18421 cttcagacat ccgttggcag tgggtgttta tcggcacggc ggtggtcttt ttcttccagc
    18481 ttttgcgacc ggctttccag aaagggttga aaagcgtttc cggaccgaag tttattctgc
    18541 ccgccattga tggctccacg gtgaagcaaa agctgtttct cgtggcgctg ctggtgcttg
    18601 cggtggcgtg gccgtttatg gtctcacgcg ggacggtgga tatcgccacc ctgaccatga
    18661 tctacattat cctcggactc gggctgaacg tggttgttgg tctttctggt ctgctggtgc
    18721 tggggtacgg cggcttttac gccatcggcg cttacacttt tgcgctgctc aatcactatt
    18781 acggtttggg cttctggacc tgtctgccga ttgccgggtt aatggcggcg gcggcgggct
    18841 tcctgctcgg cttcccggtg cttcgcttgc gcggcgacta tctggcgatc gttaccctcg
    18901 gtttcggcga aattgtgcgc atattgctgc tcaataacac cgaaattacc ggcggcccga
    18961 acggaatcag tcagatcccg aaaccgacac tcttcggact cgagttcagc cgtaccgctc
    19021 gtgaaggcgg ctgggacacg ttcagtaatt tctttggcct gaaatacgat ccctccgatc
    19081 gtgtcatctt cctctacctg gtggcgttgc tgctggtggt gctaagcctg tttgtcatta
    19141 accgcctgct gcggatgccg ctggggcgtg cgtgggaagc gttgcgtgaa gatgaaatcg
    19201 cctgccgttc gctgggctta agcccgcgtc gtatcaagct gaccgccttt accatcagtg
    19261 ccgcgtttgc cggttttgcc ggaacgctgt ttgctgcgcg ccagggcttt gtcagcccgg
    19321 aatccttcac ctttgccgaa tcagcctttg tactggcgat agtggtgctc ggcggtatgg
    19381 gctcgcaatt tgcagtgatt ctggcggctg ttctgctggt ggtatcgcgc gagttgatgc
    19441 gtgatttcaa cgaatacagc atgttaatgc tcggtggttt gatggtgctg atgatgatct
    19501 ggcgtccgca gggcttgctg cccatgacgc gcccgcaact gaagctgaaa aacggcgcag
    19561 cgaaaggaga gcaggcatga gtcagccatt attatctgtt aacggcctga tgatgcgctt
    19621 cggcggcctg ctggcggtga acaacgtcaa tcttgaactg tacccgcagg agatcgtctc
    19681 gttaatcggc cctaacggtg ccggaaaaac cacggtcttc aactgcctga ccggtttcta
    19741 caaacccacc ggcggcacca ttttactgcg cgatcagcat ctggaaggtt tgccggggca
    19801 gcaaatcgcc cgcatgggcg tggtgcgtac cttccagcat gtgcgtctgt tccgtgaaat
    19861 gacggtaatt gaaaacctgc tggtggcgca gcatcagcaa ctgaaaaccg ggctgttctc
    19921 aggcctgttg aaaacgccgt ctttccgtcg cgcccaaagt gaagcgctcg accgcgccgc
    19981 cacctggctt gagcgcattg gtttgctgga acacgccaac cgtcaggcga gtaacctggc
    20041 ctacggtgac cagcgccgtc tggagatcgc ccgctgcatg gtgacgcagc cggagatttt
    20101 aatgctcgac gaacctgcgg caggtcttaa cccgaaagaa acgaaagagc tggatgagct
    20161 gattgccgaa ctgcgcaatc atcacaacac cactatcttg ttgattgaac acgatatgaa
    20221 gctggtgatg ggaatttcgg accgaattta cgtggtcaat caggggacgc cgctggcaaa
    20281 cggtacgccg gagcaaatcc gtaataaccc ggacgtgatc cgtgcctatt taggtgaggc
    20341 ataagatgga aaaagtcatg ttgtcctttg acaaagtcag cgcccactac ggcaaaatcc
    20401 aggcgctgca tgaggtgagc ctgcatatca atcagggcga gattgtcacg ctgattggcg
    20461 cgaacggggc ggggaaaacc accttgctcg gcacgttatg cggcgatccg cgcgccacca
    20521 gcgggcgaat tgtgtttgat gataaagaca ttaccgactg gcagacggcg aaaatcatgc
    20581 gcgaagcggt agcgattgtc ccggaagggc gtcgcgtctt ctcgcggatg acggtggaag
    20641 agaacctggc gatgggcggc ttttttgccg aacgtgatca gtttcaggag cgcataaagt
    20701 gggtgtatga gctgtttcca cgtcttcatg agcgtcgtgt tcagcgggca ggcaccatgt
    20761 ccggcggtga acagcagatg ctggcgattg gtcgcgcgct gatgagcaat ccgcgtttgt
    20821 tgttgcttga tgagccatcg ctcggtcttg cgccgattat catccagcaa attttcgaca
    20881 ccatcgagca gctgcgcgag caggggatga ctatcttcct cgtcgagcag aacgccaacc
    20941 aggcgctgaa gctggcggat cgcggctacg tgctggaaaa cggccatgtt gtgctctccg
    21001 ataccggcga tgcgctgctg gcgaacgaag cagtgagaag tgcgtattta ggcgggtaaa
    21061 ttatcgaata cccggcggcc acgttctgcc gggctcttcg tccgtataca gacttgtttc
    21121 aactgaccgg gaggattaaa accagaccac ttatttgcca cggaaaggtt ccctcccatg
    21181 cattatgccg ttgttgtcat gcttgaattg ctttgctgta ttcacgctta ccgtaccgga
    21241 caagagcggt actggatttt tatcattttc tgttttccgg tgattggctg cgtggcgtat
    21301 ttcgtcatag tgatgctgcc tgaaacgggc gctgaccgtc acgggcatac tttgctgatg
    21361 cgcctgcaga acaaactcaa ccctgagcgc cacctgcgca aattaaccga agaactggcg
    21421 attgccgaga cgaatcaaaa ccattatgcg ttggctaatg aactggcgcg tctggggcgt
    21481 tatcacgaag ctgttcccca ttatcagcag gcgttaagcg ggatattcgc ccatgaagcg
    21541 gcaatgatgc taagcctggc gcaggcacag tttgctattc aggagttcgc cgcctgtcag
    21601 cagacgctgg aagatgtgat gcgttataac cctgattttc agtcggcaga cgggcatttg
    21661 ttatttgcca gaacgttagc cgcacaagaa aaatacgctg atgcggaaag cgagtttgaa
    21721 gtattgattt cttattaccc cggcccgcag gcgcgtattt actatgcgga aatgttggcg
    21781 aaaatgagtc ggttacgtga agccaacgag cagtatgttg ctgtagttga tacggctaaa
    21841 cgctcaagac ctcattatag aaaacaccac cgggaatgga ttaagaccgc caatgaacgg
    21901 ctgaagcaga gtgtggtgca atgaaaatct tctttgccgt tagaccagtg tcgcggcaat
    21961 cttttgttaa aattgaaaaa tgtagctctc atcgggcagc aaaactggac gcagaatttg
    22021 ctgccatttt tgctcttcat ggtaaggagt tctggcgatt agctggccgt taatcgcctc
    22081 ttttcagtgc attctcggga tatccgtgcc ccatctccca ttttccctca cccgtaccgt
    22141 caccgccttg tcatctttct gacaccttac tatcttacaa atgtaacaaa aaagttattt
    22201 ttctgtaatt cgagcatgtc atgttacccc gcgagcataa aacgcgtgaa ttcgcgcatt
    22261 cggtacaaca agagagataa acgatgaaac cgttacatta tacagcttca gcactggcgc
    22321 tcggactggc gttaatgggg aatgcacagg cagtgacgac cattccgttc tggcattcta
    22381 tggaagggga actgggtaaa gaggtggatt ctctggccca acgttttaac gccgaaaacc
    22441 cggattacaa aattgtaccg acctataaag gcaactacga acagaattta agcgcgggga
    22501 ttgccgcatt tcgtaccggc aatgctccgg ctattttgca ggtttatgaa gttggcaccg
    22561 ccaccatgat ggcgtcgaaa gccattaaac cggtatatga cgtgtttaaa gaggcgggga
    22621 ttcaattcga tgagtcgcag tttgtgccga cggtttcagg ttactattcc gacagcaaaa
    22681 ccggccactt actctcccag ccgttcaaca gctcgactcc cgttctctat tacaacaaag
    22741 acgccttcaa gaaagcgggg ttagacccgg aacaaccgcc gaaaacctgg caggatctgg
    22801 cggactatgc cgcaaaacta aaagcctccg gcatgaagtg cggctacgcc agcggctggc
    22861 agggctggat ccaactggaa aactttagcg cctggaacgg tctgccgttt gccagcaaaa
    22921 acaacggctt tgacggcacg gatgcggtgc tggagttcaa caagccggag caggtgaaac
    22981 acatcgccat gctcgaagag atgaacaaga agggcgattt cagctacgtc gggcgtaagg
    23041 atgaatccac cgagaagttc tataacggtg attgcgcgat gaccaccgcc tcttccggtt
    23101 ctctcgctaa tattcgtgag tacgccaaat ttaactatgg cgtaggcatg atgccttacg
    23161 acgccgatgc gaaagatgca ccgcaaaacg ccattatcgg cggggccagc ctgtgggtga
    23221 tgcagggcaa agataaagaa acgtataccg gcgtggcgaa gttcctcgac ttcctcgcta
    23281 agccagaaaa tgctgccgag tggcatcaga aaactggtta tctgccaatc accaaagcgg
    23341 cgtatgactt gactcgtgag cagggctttt atgagaaaaa cccaggggcg gataccgcga
    23401 cgcgtcagat gctgaataag ccgccgttgc cgttcaccaa agggctgcgt ctgggcaaca
    23461 tgccgcagat ccgcgtgatt gtggatgaag agctcgagag cgtgtggacc ggcaagaaga
    23521 cgccacagca ggcgctggat accgccgttg agcgtgggaa tcagttgctg cgccgttttg
    23581 agaaatctac gaagtcttaa tcagtgtaat gtcggatgcg tttcgcttat ctgacctggc
    23641 atcgcgtgta agccggataa gcgaagcgca tccggcacag ttcaggaatt aaccgtaatg
    23701 tcatcatccc gtccggtgtt ccgctcgcgc tggctgcctt atctgctggt cgcgccgcag
    23761 ctcatcatca ccgttatctt ttttatctgg cctgcgggcg aagcgttgtg gtactcgctg
    23821 caaagcgtcg atccgtttgg tttctccagc cagtttgtcg gcctggataa cttcgtcgcg
    23881 ctgtttcatg acagctacta tatcgactcc ttctggacga cgataaaatt cagcaccttt
    23941 gtcaccgtca gcggtttgct ggtatcgctg ttctttgcgg cgctggtgga gtacatcgtg
    24001 cgcggcagtc gtttctatca aaccttgatg ctgctgccgt atgccgtggc tcccgccgtt
    24061 gccgccgtat tgtggatatt tttgtttaac cccggtcgcg ggctgatcac tcattttctc
    24121 gccgagttcg gctacgactg gaaccacgcg caaaacagcg gtcaggcgat gtttctggtg
    24181 gtgtttgcct cggtgtggaa gcaaatcagc tacaactttc tgttcttcta tgccgcgctg
    24241 caatccattc cccgttcgtt gattgaagcc gcagccattg atggcgcagg cccgattcgc
    24301 cgcttcttta agattgcgct accgcttatc gctccggtga gtttcttcct gctggtagtg
    24361 aacctggtgt atgccttctt cgacaccttc ccggtgatcg acgccgccac gtccggtggg
    24421 ccggtgcagg ccaccacgac gctgatttat aagatctacc gtgaaggctt taccggactg
    24481 gatctggctt cgtctgccgc acagtcggtg gtgttgatgt tcctcgtcat cgtgctgacg
    24541 gtggtgcagt tccgctatgt tgaaagcaag gtgcgttacc aatgattgag aaccgtccgt
    24601 ggctgacgat attcagccat accatgctga tcctcgggat cgcggtgatc ctattcccgc
    24661 tgtacgtggc gtttgtcgcg gcgacgctgg ataaacaggc cgtctacgcc gcgccgatga
    24721 cgctcatccc cggcacgcat ctgctggaaa acatccacaa catctgggta aacggggtag
    24781 gcacaaatag tgcgccgttc tggcggatgt tgcttaacag cttcgtgatg gcgttcagca
    24841 ttacgctcgg caaaattacc gtctcgatgc tctcggcatt tgccattgtc tggtttcgtt
    24901 ttccgctacg taacctcttc ttctggatga tttttatcac cctgatgctg ccggttgaag
    24961 tgcgtatctt cccgacggtg gaagtcatcg ccaacctgaa aatgctcgac agctacgccg
    25021 gtttaacgct gccgctgatg gcctcggcga ccgctacctt cctgttccgc cagttcttta
    25081 tgacgctgcc ggatgagctg gtggaggccg cgcggatcga cggcgcgtca ccaatgcgtt
    25141 tcttttgcga catcgttttt ccgttgtcca aaactaatct ggcggcgctg tttgtgatca
    25201 cctttattta cggctggaac cagtatttgt ggccgttgtt gattattacc gacgtggatc
    25261 tcggcaccac cgtggcaggg atcaaaggga tgatcgccac tggcgaaggc accacagaat
    25321 ggaactcagt gatggcggcg atgttgttaa ccctgatccc accagtagtg attgttttag
    25381 tgatgcagcg tgccttcgtg cgcggcctgg tcgatagtga gaaataagat ggcaggactg
    25441 aaattacagg cagtaaccaa aagctgggat ggcaaaaccc aggtgattaa accgctgacc
    25501 cttgatgtgg cggatggcga atttatcgtg atggtcgggc cgtcaggctg cgggaaatcg
    25561 acgctgctgc gcatggttgc cgggctggag cgggtgacgg aaggcgatat ctggatcaac
    25621 gaccagcggg tgaccgaaat ggagccgaaa gatcgcggga ttgcgatggt gttccagaac
    25681 tacgcgcttt atccgcatat gagtgttgaa gaaaacatgg cgtgggggct gaaaattcgc
    25741 ggcatgggca agcagcaaat tgccgagcgc gttaaagagg cggcgcgcat tctggaactg
    25801 gacggtctgc ttaagcgccg cccgcgcgag ctttccggcg gtcagcgtca gcgtgtggcg
    25861 atgggccgcg cgattgtgcg cgatccggcg gtgttcctgt ttgatgagcc actctctaac
    25921 ctcgatgcca agctgcgcgt acagatgcgt cttgaactgc aacagctgca ccgtcgcctg
    25981 aaaacgacat cactctacgt tactcacgat caggttgagg cgatgaccct cgcccagcga
    26041 gtaatggtga tgaacggcgg cgttgccgaa cagattggca caccagtaga agtctacgaa
    26101 aagcccgcca gcctgtttgt ggcgagtttt attggcagcc cggcgatgaa tctgctgaca
    26161 ggccgcgtga ataacgaagg cacgcatttc gaactggacg gcggtattgt gctaccgcta
    26221 aacggtggct accgtcagta tgccgggcgt aaaatgactc tcggcattcg cccggaacat
    26281 attgcgctaa gctcgcaagc agaaggtggc gtaccgctgg tgatggacac gctggagatc
    26341 ctcggcgcag ataacctggc gcacggacga tggggcgaac agaagctggt ggtgcgactg
    26401 gcgcatcagg agcgcccgac ggcaggcagc acgctgtggc tgcatctggc ggaaaatcag
    26461 ctgcatcttt ttgatggtga aacaggacaa cgagtatgag taactggcct tatccccgca
    26521 tcgtcgcaca tcgtggcggc ggtaagttgg ccccggaaaa caccctggcg gcaatcgacg
    26581 tcggggcaaa atacggtcat aagatgatcg aatttgacgc gaagttgtcg aaagatggcg
    26641 aaatcttcct gctccatgac gacaatctcg aacgtaccag caacggctgg ggcgtcgcgg
    26701 gtgaactgaa ctggcaggat ttactacgtg tggatgcagg cagttggtac ggcaaagcgt
    26761 ttaaaggtga gccgctgccg ttgctttcgc aggtggcgga acgctgtcgc gaacatggga
    26821 tgatggcgaa tatcgaaatc aaacccacca ctggcaccgg gccattaacg ggcaaaatgg
    26881 tggcgctggc ggcacgcgaa ctgtgggccg gtatgacgcc gccgctactg tcatcgtttg
    26941 agattgatgc tttagaagcc gcacaacagg cagtaccaga actgccgcgc ggtttgttgc
    27001 tggatgagtg gcgcgacgac tggcgagaac tgaccgcgcg gctgggctgc gtctctattc
    27061 atctcaatta taaattgctt gataaagcac gagtgatgca gttgaaagac gccggattac
    27121 ggattctggt ttataccgtc aataaacccc agcgcgcggc agagttgctg cgctggggcg
    27181 tggattgcat ctgtaccgat gcgattgatg tgattggtcc gaactttacg gcccaatagt
    27241 tttcaacggt atatcaggct gcggcgtgcc gggctgattc agcatgtcgc cgtttctcct
    27301 ctccggcaac atatgctgct gattcaacga actatccgga ttacggttgc tgtttaacat
    27361 cccgccattg gtgttgggca gcatttgctg ccgggcggga tttcgctccc ccggctgtga
    27421 ctgcaacacc cgctgagaat tattgtttat ctggttttct aaatgctgtt gttgcaactg
    27481 agtttgcgtt ttcagttgct gattcagcat ccctttttgc tggatttgct gcgtttgcat
    27541 ctgggtttgc atccgctgct gactggggat ctgatacccc ggctggttag ggttgttcag
    27601 agtattaatg ggctgtgcaa agccgacaaa cggcaggagt gccgtaagaa tcagaagtcg
    27661 tttcatcgcg tatcctcctc tgaagatatt ctttaagttt actcctttcc tgcaatcatc
    27721 aggattaatt cagagttata taccaggctt agctggggtt gccccttaat ctctggagaa
    27781 taacgatgat aaaaccgacg tttttacgcc gggtggccat tgctgctctg ttcacaggaa
    27841 gttgttttag tacggtagcc gcgccacctg cgccgtctcc tgtttcttac ggcgtggagg
    27901 aagatgtctt tcatccggta cgcgcgaaac aggggatggt ggcatctgta gatgctactg
    27961 ccactcaggt gggggtggat atcctcaagg agggcgggaa tgccgttgat gccgccgtgg
    28021 ctgtgggcta cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc ggtggtggtt
    28081 ttatgctgat ccgctcgaaa aatggcaata ccaccgctat cgatttccgc gaaatggcac
    28141 ccgccaaagc gacccgcgat atgttccttg atgaccaggg caacccggac agcaaaaaat
    28201 cactcacttc gcatctggct tccggcacgc ccggtacggt cgctggtttc tcgctggcgt
    28261 tggataaata cggcacaatg ccgttgaaca aagtggtgca gccagcgttt aaactggcac
    28321 gcgatggttt tatcgttaac gacgcgctgg ctgacgatct caaaacctac gggagcgaag
    28381 tgctgccgaa tcacgaaaac agcaaggcta tcttctggaa agagggcgag ccgctgaaaa
    28441 agggcgacaa gctggtgcag gcgaacctgg caaagagcct ggagatgatt gccgaaaacg
    28501 gcccggacga attctacaaa ggcacgatag cggaacagat tgcccaggag atgcagaaaa
    28561 acggtggcct gatcactaaa gaagatttag cggcgtataa agcggtcgaa cgcacaccga
    28621 taagcggcga ttatcgcggg tatcaggttt actccatgcc accgccatcc tccggcggga
    28681 tccatatcgt acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggttttg
    28741 gcagcgccga cgcgatgcag atcatggcag aagcggagaa atatgcctac gctgaccgct
    28801 cggaatatct tggcgacccg gattttgtca aagtcccgtg gcaggcgctg accaataaag
    28861 cctatgccaa atctattgcc gagcaaatcg atatcaataa agcgaaaccg tccagcgaga
    28921 ttcgccctgg caagcttgcg ccttatgaga gtaatcaaac tacccattac tcagtggtgg
    28981 ataaagacgg taacgccgtg gcggtgacct atacgctgaa caccaccttc ggtacgggta
    29041 ttgtcgcggg cgagagcggt attctgctta ataaccagat ggatgatttc tccgccaaac
    29101 cgggcgtacc gaacatttat gggctggtgg gcggtgatgc caacgccgtc gggccgaaca
    29161 aacgcccgct gtcatcgatg tcacccacca ttgtggtgaa agacggtaaa acctggctgg
    29221 tcaccggtag ccctggcggg agccggatta ttaccaccgt gcttcaaatg gtggtgaata
    29281 gcatcgatta tggcatgaac gtcgccgaag cgaccaatgc gccgcgtttc caccatcagt
    29341 ggttgccgga cgagctgcgt gtcgaaaaag ggtttagccc ggatacgctc aagctgctgg
    29401 aagcaaaagg tcagaaagtg gcgctgaaag aggcgatggg cagtacacaa agcattatgg
    29461 ttgggccgga cggtgagctg tatggtgcat ccgacccgcg ctcggtggat gatttaactg
    29521 cggggtatta aggttagcgg ccctcttcgt gggaagaggg ctgttttctt gaacaagata
    29581 aattagtgtc tggattgctt ttactattgc tccgtaatat ataatcataa ttatcctgaa
    29641 tgcttttaag gggcttaggt tatgctttct tttcaagagt atgcagaata gtttttcatt
    29701 taccgtgaaa ataggacgtg tgataat
//
LOCUS       DAZGCQ010000059        28784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000059 DAZGCQ010000000
VERSION     DAZGCQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.059"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(126..509)
                     /locus_tag="ACHK07_002871"
     CDS             complement(126..509)
                     /locus_tag="ACHK07_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103181.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            complement(612..1073)
                     /locus_tag="ACHK07_002872"
                     /pseudo
     CDS             complement(612..1073)
                     /locus_tag="ACHK07_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(1498..3492)
                     /gene="cirA"
                     /locus_tag="ACHK07_002873"
     CDS             complement(1498..3492)
                     /gene="cirA"
                     /locus_tag="ACHK07_002873"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO8103182.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSTGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
     gene            complement(4183..4386)
                     /locus_tag="ACHK07_002874"
     CDS             complement(4183..4386)
                     /locus_tag="ACHK07_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103183.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(4540..5097)
                     /locus_tag="ACHK07_002875"
     CDS             complement(4540..5097)
                     /locus_tag="ACHK07_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO8103184.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELWAILAE"
     gene            complement(5133..5504)
                     /locus_tag="ACHK07_002876"
     CDS             complement(5133..5504)
                     /locus_tag="ACHK07_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFO8103185.1"
                     /translation="MMKKILLVVAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGM
                     TEGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEV
                     DISDEGNRKATVDVLTLERLR"
     gene            complement(5583..6329)
                     /gene="traX"
                     /locus_tag="ACHK07_002877"
     CDS             complement(5583..6329)
                     /gene="traX"
                     /locus_tag="ACHK07_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO8103186.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRYEHIRQASVNRLWGWAVVAQF
                     GYFLAGYPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMLCHRIYRTTHLSVRSMLALCLFPVVALVNLQDGVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            complement(6349..11619)
                     /gene="traI"
                     /locus_tag="ACHK07_002878"
     CDS             complement(6349..11619)
                     /gene="traI"
                     /locus_tag="ACHK07_002878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO8103187.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYMDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDSREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGVSKDDGLITLQDAEGNTQLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVFEPRADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(11619..13826)
                     /gene="traD"
                     /locus_tag="ACHK07_002879"
     CDS             complement(11619..13826)
                     /gene="traD"
                     /locus_tag="ACHK07_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8103188.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPP
                     GISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(13882..14613)
                     /locus_tag="ACHK07_002880"
     CDS             complement(13882..14613)
                     /locus_tag="ACHK07_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103189.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAKEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(14781..15041)
                     /locus_tag="ACHK07_002881"
     CDS             complement(14781..15041)
                     /locus_tag="ACHK07_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103190.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(15101..15832)
                     /gene="traT"
                     /locus_tag="ACHK07_002882"
     CDS             complement(15101..15832)
                     /gene="traT"
                     /locus_tag="ACHK07_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO8103191.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(15864..16367)
                     /locus_tag="ACHK07_002883"
     CDS             complement(15864..16367)
                     /locus_tag="ACHK07_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO8103192.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            complement(16383..19226)
                     /gene="traG"
                     /locus_tag="ACHK07_002884"
     CDS             complement(16383..19226)
                     /gene="traG"
                     /locus_tag="ACHK07_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO8103193.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRST
                     RGSAGMYGDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            complement(19223..20596)
                     /gene="traH"
                     /locus_tag="ACHK07_002885"
     CDS             complement(19223..20596)
                     /gene="traH"
                     /locus_tag="ACHK07_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO8103194.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(20583..20975)
                     /gene="trbF"
                     /locus_tag="ACHK07_002886"
     CDS             complement(20583..20975)
                     /gene="trbF"
                     /locus_tag="ACHK07_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO8103195.1"
                     /translation="MKEKRNDAEQKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPKLLNFHYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRDNKLREGGNNASH"
     gene            complement(20956..21242)
                     /gene="trbJ"
                     /locus_tag="ACHK07_002887"
                     /pseudo
     CDS             complement(20956..21242)
                     /gene="trbJ"
                     /locus_tag="ACHK07_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(21232..21776)
                     /gene="trbB"
                     /locus_tag="ACHK07_002888"
                     /pseudo
     CDS             complement(21232..21776)
                     /gene="trbB"
                     /locus_tag="ACHK07_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
     gene            complement(21763..22053)
                     /gene="traQ"
                     /locus_tag="ACHK07_002889"
     CDS             complement(21763..22053)
                     /gene="traQ"
                     /locus_tag="ACHK07_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO8103196.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYRKPWMAFFLA
                     ELIAAILVLFGAYQILDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            22135..22470
                     /locus_tag="ACHK07_002890"
     CDS             22135..22470
                     /locus_tag="ACHK07_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO8103197.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(22451..22792)
                     /gene="trbA"
                     /locus_tag="ACHK07_002891"
     CDS             complement(22451..22792)
                     /gene="trbA"
                     /locus_tag="ACHK07_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO8103198.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCVLPDNFLFLCGAVMNQCAVPVVSMAVGTIPVITKFERFRRIFVCIMLSSAWSG
                     VMWFFIISLVK"
     gene            complement(22806..23549)
                     /gene="traF"
                     /locus_tag="ACHK07_002892"
     CDS             complement(22806..23549)
                     /gene="traF"
                     /locus_tag="ACHK07_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO8103199.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVLAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(23542..23799)
                     /locus_tag="ACHK07_002893"
     CDS             complement(23542..23799)
                     /locus_tag="ACHK07_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO8103200.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(23813..25717)
                     /gene="traN"
                     /locus_tag="ACHK07_002894"
     CDS             complement(23813..25717)
                     /gene="traN"
                     /locus_tag="ACHK07_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO8103201.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQIGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGACLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(25811..26191)
                     /locus_tag="ACHK07_002895"
     CDS             complement(25811..26191)
                     /locus_tag="ACHK07_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103202.1"
                     /translation="MSLPAGYYRIEPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMTALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(26188..26826)
                     /gene="trbC"
                     /locus_tag="ACHK07_002896"
     CDS             complement(26188..26826)
                     /gene="trbC"
                     /locus_tag="ACHK07_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO8103203.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKLQ"
     gene            complement(27055..27387)
                     /locus_tag="ACHK07_002897"
     CDS             complement(27055..27387)
                     /locus_tag="ACHK07_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103204.1"
                     /translation="MEKKLSSKYIHIYMSISCVFIRLTGKALKLFPFALVMLFTVLFY
                     FMPENVICDIISEWQRSDVSDRIRVIRLYLGTTFLCTWAAVFLEFLCSDEFLPGKRLN
                     KNEEENNG"
     gene            complement(27414..27539)
                     /locus_tag="ACHK07_002898"
                     /pseudo
     CDS             complement(27414..27539)
                     /locus_tag="ACHK07_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
     gene            complement(27581..28027)
                     /locus_tag="ACHK07_002899"
     CDS             complement(27581..28027)
                     /locus_tag="ACHK07_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103205.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
     gene            28627..>28784
                     /locus_tag="ACHK07_002900"
     CDS             28627..>28784
                     /locus_tag="ACHK07_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8103206.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGL"
BASE COUNT         6877 a         8062 c         6364 g         7481 t
ORIGIN      
        1 aataccacat tccctgttac tgaccattcc ctccatttcc cgcttttcac tacagaaacg
       61 ccgggggcgg ggaaaacttg ctttttcccg tccccggggt tggacaactg agcaacgcga
      121 aggcgtcagt ggatttttat gatccttcca aactctgctt ccattttttt catatcctgt
      181 tctcttactg cgactatcag ttgttttcct gattttcgtg ccactctggc aaactgcatg
      241 acggcagatt cattattcaa atcaggccat cctgtacaat taaaaataat aatatgctca
      301 ctggcgttca gtgcatccct gataaaaata accgcataat catttaatgt tctgtcagaa
      361 caactgtaaa tctggctgaa atcataatac ttataatcag taatattcag tttatttaaa
      421 aagctatcaa cttcagaagc tgagtccccc acaataagaa cattatgatt agacgatgtg
      481 attaattctg atactgcctg atagttcatt tgttcacctt tcattaacag agaaataagt
      541 taataatgta atatgcttca tacttttaaa tatcgcattt caatgagaac tcaatagcca
      601 cagaaatatc attatttccg tgagcgccat acccagggcg gaacaggcgc gctgtctgcc
      661 cacagaccac gtttctgctg tcgtgcttct tcctgcagga cgggaagaac aggatcggtg
      721 ttgtactgct cataaaccca tgcagcgcct gcacggacca taaactgatt aacattcatt
      781 ccgtctggtg cataaacctg cccgagaata cgaccataac gatctcgctg aaaatatgtg
      841 acagtaaccg tttttcccgc aaccagagat tacattattt tttcagtcca ccgtccatag
      901 tcctgtttct tttcaggtgc atcaatatta acaagacgga cacgcaccgg atgctgagat
      961 aacatgacgt caatggtatc gccatccaga acacgcacga cccgtccatg aatatcagca
     1021 gaaaaaacct gatatgaaaa aacaaacaat accactggta tatatttctt cattttactc
     1081 tctccgtcac tgattacttt ttgtgattgc aggaattcca ttccgtgatg aataatgcct
     1141 cactcatcac gccaaaaata aaccgttaaa ttgtggtcta ttcagtaaaa taagattcag
     1201 tggctgaata cacatatagc catgtgttac taaggtctca tgaattaatc atgcattctg
     1261 tacgaaggtt aacgtatgtt ttttagttaa tagtagcctt aattaccttt cagatatatg
     1321 ggataatttc ataatgaaag accgcagaaa atgaattctt ataaaaatga actttacctg
     1381 attccgtaaa tataatttac tttctttatt catccgcatc ttacttcatt cttatcgtct
     1441 tgtttatttt cagaatatta tcttataaag gctcctcctg gagccttcgg tttactatta
     1501 aaaacgataa tccattgaca gaaaatatct tctcccgtca acggtataac tgtaatcatt
     1561 ccgttctaaa tctttatcca taacatttaa tatacctgaa cggacagtta tatttttcgt
     1621 agccttccag gaagctccgg catcccataa tgtatacccc ccctgcgttt ctgtgccgga
     1681 agattgtgcc cgggtttccc catggtaatt agctgatacg taaaaagacc agtcatccac
     1741 aggagtccag tcaattcttc cgtttgcatt atgaaacgga ctgtcattca gtggcttatc
     1801 ttctccgcta ctcaaatccc gtccatctgt ataggtatag gtgagagaca gtttcacctg
     1861 ctctcccagc gggattttca gttctgattc aagcccctgt atcctagctt tatttatatt
     1921 aaagtatctg aaaacaggcc gtccatcatc cgttatcccg acaaagttag gcagcgtcat
     1981 tgcctctttc agtttcttac tgcgtttgtt cgcaatcatg tcatcaattt cattacgata
     2041 cacagtgaca cttgcttcca caccttccag gagtccttcc tgtccgctgt aataaacacc
     2101 accctcaata ctttcacttg tttccggttt caggtcgggg ctaccgatga tctgacatcc
     2161 ccctttgcat gagttcgtca gccatgcttc actttgctga atcagggtcg gagctttaaa
     2221 tgctctggcc catcctcctt ttacagtaac agaatcggtt gcattccata caagatatgc
     2281 tcttggactc cagttggttc cataattttc gtgcttatcc atacgaaccc ctgttgtcaa
     2341 agccaggggc gcgaacaaac gccattcgtc ttctgcaaaa agtgaatact gacttgtcga
     2401 attttctgaa cctttctctc cggtaaggtg gacaggatca cttattttct ccctgcgata
     2461 atcaccacct aatatcacga tatgattata atcagtgaat gggaatacat attttccatc
     2521 aataacctca ttccatgaag ttatcggatg tgtattacct gtattcagat tatttacttt
     2581 ttcactgtaa aatcggagtt cactgttccc taatccccat tgtccattat ggccaacaga
     2641 ataattctgt cgttccatcc ggtttttatc actactacct tcagactccc ggtcctgacg
     2701 gtcaaaagaa taccctgtgc tgaactcgtg attctccaga ggagtaagcg taaagtcaac
     2761 gccaccatta cgggttgtaa aaccttcagc acgtgtagtt tcctgtccat ctgccgattt
     2821 ttgttttccg gtatctttat cccggccact taaagctccg taaactttaa ttcctaaaag
     2881 gttttcaacc aaaggcccac ctgcactgaa ctgtccccca taagtatcgc cacggtctct
     2941 gtgttcctgt aacgtcatat cccccgagat gacacctgac cattccttac ctgttttttt
     3001 agtaatgata ttaatgacgc cccccatcgc atccgcacca tacagtgatg acatagggcc
     3061 acgcacgacc tctacacgtt caatagtgga aacagggatc cagctcagat caaagtcatt
     3121 attccggaac atagctgtgc gggaactgac ccgtttacca tccacaagaa tgagggtgta
     3181 tcctccgccc agtcccctta tatttacgag ttttctgtta tcatcggtgg taatctgcac
     3241 cccgggcaca tcccttaaaa catccaccag attatggact ggcttacggg ataaatcatc
     3301 ctggctaata acactgatac tggcaggtgc gtctttcaga ttctgttcaa cagcagatgc
     3361 tgtaactacc agagtatctt cagcagcgtc cggaagtgtc gaatgtgcag aagtgaacac
     3421 aggaaatgaa aataacgatg cacctatgct ggttgaaaga agatgacgaa taaaagggtt
     3481 aaatctgaac atttctgatt atatccataa tttgtattca atgttgcaag atttacccac
     3541 actgacttcc ataaatatga tggagtttgc cccatcttat attaagaata ttgatatact
     3601 tctttgatta aaatttattt attgatattt ttatgtcaat accattcttc tctactttac
     3661 tatacatact ttcctgttcg caaggtcatg tattcccgtt ccccgttcgt accctatcgg
     3721 cttccccctg ctacatacta ctttgtgata cagattatat ttttcacccc tgagtattaa
     3781 acattcctgc tcttgctgat aggataactg tgttatatcg caggctaccg gatgtggcgc
     3841 tgaatataac ggtcatagat aagtcataca tgccctgctc aggggattag cgataagacg
     3901 gaccgggaga gtcatctccc ggtttttctt tatcctgccg cgaatacagc ccgactgtct
     3961 gtcagattca ccggataacc ctgctgttat gcatgccact gtcacctcct gcttcttgaa
     4021 attcatcaca ttttttatcc ctgctggctt acccgggatg tgaaatatgt taactctgtg
     4081 atgtccgttt gaaagacagg gcaactcctt cttacaccac tataataata acgatctgcc
     4141 atataagcct gatcccccgg cgctccgcaa cgggggttat ttttatcggc acttacaccg
     4201 gcatgcagat gctctcttca gggcaaaatc ccgccatgct tcacagggtg ccagccgggc
     4261 cgcattctgc caggcctcag cggcttccct gaaaaaacca cgaatctcct gacggacggc
     4321 atgacgcccc acgcgctgat agcgataaat accgctgtgc tcagataatc cactggatac
     4381 agccattttt tatcccccct cttgacccgg ctcccggatt atgtcagaaa cattctgaaa
     4441 catccctgaa cggttgggtg tttttctcca tcaaaactct ggatgtctgt tttcccgatt
     4501 ttttcgctgt tgccctctgt catacagagg gcgtttttat tattccgcca ggatcgccca
     4561 cagctcatcc ttgatcctgt tctgtctgcg caccttttcc aggcgcgcct gcgcatactg
     4621 ttcttcttcc tgtgtgatat gttctgtcac atagccttcc gtatcatagc ggcaggcgcc
     4681 ggctttcatt gcgcacagat aactttccga acgggtgatg gctttcagcg cccggctgag
     4741 ttttttatga gagagcggga tatttcgctg agacacctca tccagtaaaa cctcccggat
     4801 accgcaggca agaagccggg gcgtgttacc gtcaaacaat cccggccacc agggtttcag
     4861 agtgttcacc gcctcatcca gcgagggcag cgtcagataa atggacagcg cctgtctggc
     4921 ctgtgctttc ttcgccgcca gctctgcttc ccgggcagcc ttctccgcca gtttctgctt
     4981 tttcaccttc cattttggtg gcgtggtgac attgatgatg gttttccggc tccgggtcgg
     5041 tgtttccacg tcggttcttc gtttcagtgt cagtactggc cgtttctgct ctgtcataac
     5101 tcacctgccc tcaccggtat ccgttgttca tatcagcgaa gacgctcaag agtcagtaca
     5161 tccaccgtgg ctttccggtt tccctcatca gaaatatcca cttctcccac aacctgaact
     5221 ttatctccgg ctttcagggc cagcgggcgc catacttcat catcgacatc aatgacaata
     5281 cttcctgatg catcactgag ttcaaaatga tcctcttcgg ctgccagttg cttcactatc
     5341 tgaccattca gcgtcacacc ggtcccctcc gtcatgccac tcacctgaga aacagtgatc
     5401 acattatttt tagcctcatc ccggatttgc tgatctgatt ttgcccatac cccctgaacg
     5461 aacattcccg taatggctac caccagtaat atttttttca tcatctgctc ctgttttttc
     5521 ctgaatgcgt tctgatttaa acaaaaagac tttcacagtt catccgcttt ctgcgcttcc
     5581 tgctacaggg ccatcactcc cagcacagcg agatgacaga cataaaatac cgggaaaaag
     5641 tctgccggcc agaaacgggc agcaggccac cggataccgg ataccatcct gacgaccatg
     5701 tatgtcacta acacaccact gacagcggca gtcacgccgt cctgcaggtt aacgagagca
     5761 acgaccggaa aaagacagag cgccagcatg ctccgtacag aaagatgcgt cgtcctgtat
     5821 atcctgtgac agagcatcag taatatgatc ccgacagcac cgtaactggt ggaagataac
     5881 gggaaccaga gaaccagaag tgtaagtgca ctgactgacc ataccagcga gtgatattca
     5941 caccatttca gcatctgtgc tgccaccgca aaggcaaaca gaatattgcc ctcataccac
     6001 ggatatcccg ccagaaagta cccgaactgc gccacaacag cccatcccca caggcggttt
     6061 acggacgcct gacggatatg ctcataccgt gacagattca gtccccagac cagcgcaaac
     6121 agtggaaacg cgccccgccc ggccagaaac atccacgtct gctccagatg cagaatgcgg
     6181 tttgcatggt ccagcaccat caacaccagt gccaccgttt tgatgatatc ccgctgtccg
     6241 ggtgaccaga ccagcaacga ctgcagccag gtaccggtcc gggcagccag tgaatcgtta
     6301 cgtgtcgtgt tcgtgttatc cgttgtcatc agcctttgtc ttcctgtatc agtctccgcc
     6361 cagggttttc tctttctgca ggtcacggac catatcccgc tccatctgct gaagtcgctc
     6421 ctgcagcaga ttttcccggg caacctcccg gacagcgtcc tgcacccgtt gaggctcacg
     6481 cagcacactc tccggcagcg cagtttcccg ttctgatgac gaagccctgt cctgctcctg
     6541 cccggcaata gccctgacag cctgctctgt tttaccatcc ggctggtcag gtttttcctc
     6601 tgccattttt ctgacaatct catccgcacg gcgttccgtt tcacgacgga tggcctcttc
     6661 agcctgccgc tgtgccagca cctctgccgt gaccggctca ccgtttccgg cccccggctg
     6721 gacgctgttg tccgggatat ccccccacac gcgaccaccg gtgatggcac cgggattcca
     6781 cggacgacct tcaccggcga ttctcaccac cacaccgctg tcggggttat cacgggcaat
     6841 ccggacacca tcctgcatat tatccgccag caggctctcg ccgttacggc tgccctgcag
     6901 ggccacaaac tgtgcatctt cactgccctt cacccggcct tcaccactga atccccgcag
     6961 cccgtttccg tcatccgtgg tcagtgggtt cagccagata ccggcggact tgccgttacg
     7021 gtcaaaagcc ggcagggcca catacggctg cggatatttg cgccccggtg caataaaccg
     7081 tgcaggactg tctcccccgg acagccctgc ctgacggaga acagcacgac cagctgccac
     7141 atcccgcagc tcccgcgccg tactgaacag ccgctctgca ttcatgacct cccggtcagc
     7201 ccttggctca aacacatcgt gggccgttcc tttctgtacg gctttgctga tggcatccgt
     7261 ccagccctga cggttatcgg tgtacacctg cacatgctgc ttcatacgcg acagggccac
     7321 ataggctgac tcaaagccgg ccatctgttt ccggttacct tccgtgcctt caagcgcgat
     7381 ggcaaaggtt tcactcgccc cctgcgcacc gtgagcggtg atggcatagg ccaggtcaat
     7441 atgttgctct gcctgctcct ggccggggcg aatcacccgg gtctgctgtc cgtccgacag
     7501 cgtgacactg tcaccggaaa ccgctgtcac agtccagacg ctgttggcca cataaccgcg
     7561 ctcccggtca ctcttcgtga agcgcatccg gtcaccggtt cccacccgga tggtgtccgg
     7621 ggtgtacagt gtgacgcctt cagccactgc ctcccggggc gaaatcagct gcgtgttacc
     7681 ttccgcatcc tgcagggtta tcagcccgtc atccttactg acaccggcaa tccggtgata
     7741 cacattatcc accagggcaa gggcatccgg gttattctcc caggtggaga gacgacgcag
     7801 ttccccgtcg cgtatattcg ctgtattcag gacaggcacc atgacctgct ctttccccag
     7861 ctcaccggcc ttttcccgtg aatcatgaat catgctgttc agtacgcgcc ggtcctcatt
     7921 caggtgcgtg acaatcagcg tctgctcccg tacttccggt gtcctgccgg tatagtcgcg
     7981 gacaatggct tcatacagtg tcatgggaac atccgggaaa gcctcgcctt tcagcatcgc
     8041 cttctgctgc gcttctgcca gtttcgcttc ctggctgtga ctgaactccg tcacggagtg
     8101 ctccggtgcc catgcaccct ccagacgtgg cacctgagac ggtttcacac tctcaagccc
     8161 ggacagtgcc ctttccacat cccggttaat caggctgtat accgcctccc gcagttccgg
     8221 cgtctgacgc acaatctctt tcatgatggc cacatcggca gcactgcgcg tctgctggag
     8281 acggaaaggc tgaccgggcg cgatggcctg cagctggtcc gtgtcaccgc tggccacggc
     8341 acgaccacca ccggccgcaa tcagggcgta tgcccgtgcc atgtcggtat tgcccaccat
     8401 agagctctca tcgagcagga acagcgtgtt gctgaaatcc ggcgtttctc cgctgcgctg
     8461 ctgcagctgc gtgtcatgca gaaaagacgc cagtgtctgc gcatccacgc cggcgctgcg
     8521 catctccccg accgcacggt gcgtgggccc cagccccacg acacggggac gctcactctc
     8581 cggcagcatc ttcacggctg acatcaccgc ccggaactgt gtggtcttac ccacgccggc
     8641 ataaccctgc accaccgtga aacggtcgga cgtttccagt atcatgcggg tggcggcacg
     8701 ctgccccgac gtcagtgtct ccatgagttc gccgggtacc ctctccatca gcggcgtgac
     8761 cgcctcctta ccttcgagaa tatggcgaag aatgctcttt tctgcctcat acgacgcacg
     8821 ggaaaccagc aggcctgtgc catagccttt tgccacatcc acatacagta aatcaccgcg
     8881 ttttatctgc gcattgattt cccctcccag ctcagtaaaa ctggttcctt ctgcagcaaa
     8941 cgacttcgcc tctgtcagca ggtccaccat gctgaaggcc aggtttttac tttccagcac
     9001 aggaagggcc agatgaatgg cctgctgtgc cggcgtgtga agtgcggact tctgatgact
     9061 gatggcactt tccagcaatg tctcaccggc acgcgccttt atctgctcag aaaccaccgt
     9121 aaaggacgga tggcgggcga gtttttccgc agtacgggtt tcatccagtg aggaatacag
     9181 ccggacatca cgaccgctgc gggccagacc gttcagggtg gcgttgtcca ttgccatctg
     9241 tgtgacggag gcaaaaacct tcgcactgtc actgacggaa tgcccgggcg tttccaccca
     9301 gccattctcc agcttcagtg ccgtgaacgg tgaatcagcc acaggcaggg tggccggttc
     9361 agcccgcccc ggcacaacaa ccgtcatcgc atcttcactg acggatgcca cctgcaggcg
     9421 gtcaccgccg gagacgcgga gtccgggaat tttccctgtc accctcagtc gctcgccgtc
     9481 tgccaccggc attttttccg gccggaacag cgaccagctg ctgtccaggg aggatatacg
     9541 caccacctgc gtttcgccct gcgcatcccg cagggtcagg ctgtgactct gcgctgtcac
     9601 ccggtcgata acatagcggt catgactgcg tgtctccggg ttccactgct ccatcaccat
     9661 tcccgggcgg tacatatccc gcagataacg gctccggctg tccagccaga ccggtgacag
     9721 caccgtcatg gtcacctccc tctgcccgag tacgccctgc gttttcagtt cactgcgaat
     9781 ggcctgtgtc agtatggcct gttcccgtac tccgctgacc tgtgccacgc tctcttctcc
     9841 ggctttcacg ctggccgcaa aatctccggc cagccgggca tagcggacat tacggtccgg
     9901 ttcactgatg atggttgccg gtcgctgctc tcccccctgc cagcgatata tgttcacccc
     9961 ggcatccttc atggccatca gcgcactgcc tgtaccggtt cgctgcccgc tgtcggttat
    10021 caggacctgt acgttatgac gtgcggcacc gtccagcagg gttaacgtct ctttcaggga
    10081 gagtttttcg ccctggtcga cgataacggt actgcccggc gtgaaggcca tgccttccag
    10141 cagctgacga cgtccggtta tcagttcacc ggacagccgt tcatcctgct tcaggttcat
    10201 ctgtgagcga cggtcagcgg caataatctg cacctcccgc ccctgctccc gggccatcat
    10261 gaccagttca gccacccgct cacgctgccc ggctgcaccg ccctgcccgg acacaatggc
    10321 cagcgacggg cgatcctgtg ccagaacact gaccgcatcg ctgtaaccgg ccgtccgggg
    10381 aacacttttc tccggatgta cagtcacccg gttctgtttc atgatgtcac gactgagtgc
    10441 ccggacggac agctcatcga gcacatgaat cccggacgtg aacagcccct tctcacggtc
    10501 gagggggata agctgctcac ggctgatggc ctcatcgata ccggcacgcg cccgctcaat
    10561 cacaccggct tccggcggca gtatgccgac cgtcctggcc agcacgtcca tgtacgtgaa
    10621 ctgcactttt cgttcactta atccggcaat ggcctgtgtc accgcctgct gcacatccgg
    10681 cccgtcctgt gaagcgggtc cgggtgtctg cgtgcgggtc tccgcccgtt ggtctgccgc
    10741 gtcacgatac gcccggatgt cgaacccggt ttccttcagc gtctgcatcc attcagccat
    10801 tctgacttca ggatcgacgt gctgtttgga ttttcgcgta tccagtgccg ccacatcccg
    10861 ggatttcagc gaggcgtctt ccccgacggc ctcccggata gcctgtgagc gtccggaaaa
    10921 ggcctccacc ggtacgcccg gcatctccca cataccgtgc ttacccacca cttcagtttc
    10981 atagcccagc gcctcaacgc gctgacgtaa ctcactctga tatattttcc cgaacgcaat
    11041 acggttcgcg agcacgttct cggaaaaacc ggttttcccc actttatcac tgctcagtgt
    11101 cttccattcg ccgttatgct gcgtgacatt agccaccacc gcatgcgtgt gtaactgtgg
    11161 gtcctgatcg cgactggtgt cgtggttaaa gagtgccatc accagattac cggtcagtac
    11221 cgtttctgac tgtccgtccg tcatcacccg tgtggaagcc agcgcctcca cctgacggac
    11281 agcaaaatcc acggcctgat tatgtgcatc aatcaggcgc ttatcgccac ctaacatggc
    11341 catcatggag acacttttgg gggcggagaa ggtcaggtca tagccgggac gatgcttatt
    11401 actgccatcc tgcatacggc ttaaatccgc cccgtccggc agcttcccct ccagaagacg
    11461 ggtaaagaca tccttatcaa cactgccctg cagcccaagc tgttcagccc ccttgcccgc
    11521 ccagcgttcc cccatgctac ccagcacata gtaattatcc ttgtcggtgt aatagtttcc
    11581 ggcactgcct gccgatttaa caacgctgaa actcaacatc agaaatcatc tcccggctca
    11641 acatcctccc cgcgctcccg gtgcacgttg atattcacct cttcacggcg ctgcatctgc
    11701 tgctggatat ccggattctg ctcccgttgc catgcctcat aaacggccat atccaccact
    11761 tcaccggatt cactgatccc gggtggcagt tgctgttcca tctcttcttc cggtttcatt
    11821 ttcagctcct gctcgatccc ccctgccgga acattcacac ctgcatctga cttcttatcg
    11881 ttgatggcag gagacaccgg ctgttgcggc tgttgcggct gttgcggctg ttgcggctgt
    11941 tgcggctgtt gcggctgttg cggctgttca gcctgagtca cgtcttctcc ggaaacaacc
    12001 tccgggacat ccggttcgaa gaggctggcc atctgacgac cttctgcttc ccttgcggca
    12061 agtacggcac tcagacggtt ctccatttca ggattgatgt cacgcggaat aaactccgga
    12121 gcaaccttcg gtcgtgcctg atatttcagg gagagtttta ctgccggata cggtccgggc
    12181 agggtgacat aacaggtcag atccggcaga gactgaatgt cggaataact gaccagcgtc
    12241 tggcgctcca tatccttacc ggtcgatacc ccgtcacgta ccggatcagc accgtaggaa
    12301 tactgctcgc tggctttcag gtgctctttc tcaccaattt caccggcagc gaactctgca
    12361 atcttatggc tgggggaacg gaaaaaggca cgggtgttca tgacgtcaaa cagcgaggct
    12421 gccgatttct caccgtagat atcttccagc tgggcatagg actggatacc gaacacataa
    12481 cagccaccga acttacgggc ttccggcagg atctccacca ggtccggcag tttgtgtaac
    12541 gtgggtaact catcacagaa gaaccacaca cgacggttac ggttctcccc catcgccatc
    12601 aggccacgaa tggcgatgga cagccacatg gagatcaccg gtttcaggga agcatgggtg
    12661 tcggcattcg acgaaataaa cagccagccg tttttcttat cttcacggac accccgcatc
    12721 cagtcacgga tggtgaagga ctcaccatta tgctcaatcc cctgcaggta acggatggct
    12781 ttcacgtagt tggtcagcac agcacggatg gaaatcgccg ttttctcgat tttctcttcc
    12841 accaggttgg ccgccggtga gttacgcagg aaggtacgca gtttttcaat tttgatggag
    12901 agtagtgtat ccaccagttt gctgtagctg cggttggggt cattacgcat caggtacgcc
    12961 gcttccgcaa aaatggtgcg tcctgagccc tgccagaacg ggtcttcttt tgtgcccatc
    13021 gggatcaggg tatttgccgt attatcaaaa tccggctgtg tcaggcactc cttccacaaa
    13081 tcccaggcgg cacagcgggc atccagcgga ttcaggatat tatcgatgga cgggtcatag
    13141 taacttttaa caaattcccc tgatcggtca taaatcacca ccatatcacc gcgctgacgg
    13201 gcgtagttgg ccagacgacg gataacttcc gacttaccgg ccccgaccgt gccgtgcaga
    13261 cagaaattct ggatttcaga atcccggata atcggcagat cgccaatccg gatatcggag
    13321 tccttgccgt cttttttcag catcctggca acctctttcg gattgtcagt cagctgacga
    13381 ccacctgtga cttcattttc actctgctgt ttaccctgac gccccagaat ccaggagaca
    13441 acaaagaagg tgatcaggca aataaccagg gccacgacag acgccagaac gaatgcagac
    13501 catagctgct caccgcacca gatcatgtat ttatcatgca gtacctgagc cgcgttcatc
    13561 cggaaggttt tgccgtaata ctggatctca tagaccggct gggacctgat taaatcccgc
    13621 atgccttcca gcgtggtgca ccaccagtaa atacagccgt tcacaaacgt ctgccagctt
    13681 atttttaccc ataaaaccag accaacgagg atccagaaaa aaataaacag gcagtaaagc
    13741 atgatattgg cgatctggct gaacatgcgg atacgcatgg acgcaatctg accgccctgg
    13801 gtcatatcct ttgcgttaaa actcatgatg aaactccggt aaagtcaggt acagagcaga
    13861 aaagagcggt gtggaataca ttcacagaac aaaccatgta taccctgtat accggtggtg
    13921 atagccgcct gctggtctgc cggaagaaac tttttccagc acgatatcag tcaggcgcaa
    13981 tatttcagac cagtcactct cgtgtgaata ctggatctga acaaaaaggc gtacccgctc
    14041 acgataatcc agagccgtgc ccggttcata aaagtggcca ctgtcaggag aaacacggga
    14101 tgttttattg atgagatcgt atttatcggc atgagtctgt gacgacagag accaggtaat
    14161 attttcttcc tgctcaccat caatacggct actcccgggc gtccaggcaa tttccttcgc
    14221 actggtcacg atgatctgtc tggagacttc tgccagtttt ttgggggtac agaataccgg
    14281 ggccagcaca cacagggaca acaaattaca gcggccctct tccggcttaa gcggattgct
    14341 cccggatttc tcaggaaaag atatttttgt cagtatttca gttaattcct tatgcctgag
    14401 agaattaata aagtacatga caagcatcat gagaaacaat ataacaataa gccctccgat
    14461 aaccactaaa aggtcagtat caatattatt gctgtacaga taatcttttg tggtcgctgt
    14521 attatcctgt atcaccgggg agtattcagt tacaggcttc tcagtaaatt gattattctc
    14581 accaacatat tcagcgatat tgtctgatgt cataatgact ttctccggaa gcttccggct
    14641 tcgccggcaa cacttcacgg tcgcacaaag aatttgcaca accgttcagt gttctcatat
    14701 ttatttcgga aacgccggga agtgacaaaa tttattttgt tactttccgg ggttggacaa
    14761 cgagcaacgc gaggcgtcag ctattttctt ctgcgacgtc cggtcggata ttcactcaca
    14821 ggaagatatt ctgttgcgct tacgccgatc tcatatgcag agaacggttc cagtgctgtc
    14881 aggtatctct ggcaggctat ggccagttca gaatctccgg ttgcatcagc aaaccggtaa
    14941 agttctttta ataactcccg acatacatgc tcaggatact tttcactgtc atagcccagc
    15001 agaaagagta cctgcgagag acgagattct gcacggttca tgtattcatc cggggcagga
    15061 atatcggccc ggtacaatct gtccgggccg gatgggtaat ttacaggatg ttcgcgatgg
    15121 atttagccag ctggtcttcc agtacaggct tcgcttcttc aaatttcagg ttaaccttgt
    15181 tggcattaga taccacacgg gtctggtatt tatgttgatt tccggttgat gtgctggtct
    15241 gcacttttgc accggaggtt ccctggcgga gagcagcaac attatcagtt ctgactttag
    15301 atgttgtccg ctctgcaatc tgcacatccg tgatcatggt gtagttgata tcctccacca
    15361 ttgcatcggc agccatacca atcaaccctg tggcaatccc tacacccagc gccgcaccgg
    15421 cagagctgga gttatatcct gtgatgccgg tgcccaatgc tgcaccggca atggcacctt
    15481 cataaccgct gttcagccag gtctgggact cccgcaaatc cattttgtct gccttcagga
    15541 cattggcctg gatccagtag tatgccttgt caggtgaggt cacaacctgg tagcctttgg
    15601 ctttaacggc atcactgatt tttgcctgca atccactcat atccttgtca gatgtgtttt
    15661 tgatttgcag gaaaacggtg cgttcactgg aaggctccag ccagatggtt tcactcatct
    15721 gagttttgac ttcaagatta cgcttcttga ttgctgtgct catcgcacca caccctgaaa
    15781 ggaccagagt ggaactgacc agggtaacca tcatcaattt ttttgttttc atcaaattac
    15841 ctttattaca agctacagtt ttcttatcgc ctatcatcat agtcctgcca acaagtccca
    15901 aaagtatttc ctgctgaatc aaatcctcca tgcatagcta atcctgttgc cggattagta
    15961 cgataaaaat ccacataatc aaaattcaca tcatcaaaat ccagatgcaa ttcttcattg
    16021 gtgttttcat ttacctgaga ttgcatccat tctcttacac ttacacaacc acaaactcgc
    16081 cttaattcgt atagtgtacc aatgataaaa gtcatcaggc ttaataacgg agagaataaa
    16141 gaagatactg gcatcagtat ataccaaacg cctttactga atttaaactc atccgacaca
    16201 tgccgttttt ttcgtatttg agaaaacaac catataaaca atccctgcca tagtacaata
    16261 aagcacagga taaaaactgt ctcagcgccc ccccaatcag ccagattaca ggcaatgagc
    16321 tgtatcacag ttatcagagc aacatgagct aaatttttca ttatcatctc ccaaacccag
    16381 cattattgct tatacttatc ctgatactct tttgccctgt tcataagttc ctgtgggctg
    16441 tcagcaccgg gcattccttc ctgagctgat ttttcttcat tatatttatt attctgagat
    16501 aatgcctctg ttttatggtg attttgcagt tcagaatact gccctcttac aatattttcc
    16561 tcgccgtgga tgctgttctg agtatccccg atattgcccc gatattctgt gaccatatta
    16621 tcaacctgct gcttcacatc gttacggata ttactgtcct gcgttctttg ctcaataata
    16681 ccctgatgcc cctgatggtc tgcaataata tcctggctgc caccgcccga aggtacgctc
    16741 tccattcctt taccgatatc gccacgggat tcacgccagg cgttatcaac accaggctgt
    16801 acctgttcct gaacaaaaga ccaggccatt gcccgacgac gttctgcaat ctccggtgaa
    16861 ctggtgtttg tcagaatagc ttctgcatcc tgcggcgcgt gtttcatcac atactgtgca
    16921 aactgttgtg acaggtcttc actcatctgc ccactcatgc tttcagtacg ggaggccatt
    16981 tcagcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact ctgtttcgct
    17041 gagttcaggg ctgcagaaag ctggtccacc ctggaatcgg cattattgtc agtatggctg
    17101 ccggattcac tgaccttgcg actggtaaag taatcgcttg cttctttaaa gtcttgtgta
    17161 gctctggcat caatatcatg acgggcatca tggctggccc gtgaaccact gctggcctga
    17221 tgcgcatcct catcattcca gtcaatactt gtcctaccac cacctctgac acctgcgcca
    17281 ccaccaacac caagaatttt aggtttaacc ccccattcag catgagcatc accatacatc
    17341 cccgcagaac ctctcgtact tctggatgcc agttctcttg tcgcctgctc atcgctgata
    17401 ttatgcgcct tcgcatagct ttccaccgca ctgcgcatac ggctggccat catggagtcc
    17461 tgcgcgctca tcgtgctgtc agcgccactg gtgactgaat cactgcttcc ccggttagag
    17521 ccaaactggc tgagtgtgtt ccaggcactg gcaatactgc tgctgaaacc atgcagggca
    17581 ctttccgcct tattcatcga ctcccgggcc atttcctgtt gcgctgctgc aatctgacgc
    17641 gttgcattaa taccaaccgg caaacgggac attgccccac tggcgtccat caccatactg
    17701 ccgtcacggg tctgcgtggc ggttgcacca ctaccggtct ggtgcgtcat ctgaccaaat
    17761 gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tattcgtctg catgttgccg
    17821 taggagtaat taccgtcaac cacactgcct gccgcactgg cggttggcgt gatggaagaa
    17881 gacgcaaagt ggctgtatac gctggaaaat ccggcaccaa ggcctcgtac catcatccat
    17941 gacagagggg ggatcattgc tgaaagataa cctgcggttg atgccaggtc ggagtatttc
    18001 agctgtatct gagagagttc agagagcacg accggagccc cgttctgctt cgcatagaat
    18061 gtcatggcac tgttcaggat ggcatacagc aacggccagg tctggaacca catcagggca
    18121 aacacatagc ctttcaggac ggacagcgtc agtttgttga agaccgcagc cagcaccagg
    18181 agcgggaaga taccaatagt aatgcccacc agaatggtct gtaccatcgg caggtttcgc
    18241 atcagcacat gaccaatgga gacatgtgcc agacgctgtt tctccatcga tgaggtggtg
    18301 gccagattca ccaggctggc ggtatcaccg ttacgggccg cattactggt gataccctct
    18361 ttcagggcat tcatggtgac gttctggcgc ataatctggc tggcagactg gctggagccg
    18421 tagaaataac tgtaactgtc actcaccagt tgtctgaata acaggtcagg gtccggcctg
    18481 ccgccaaata tctgctgcac ataataattc caggtcttgc ctccggtatt cgtatcaaaa
    18541 ttcagcttat ccttcagtcc gacggatgcg tccttacagg acacaaacgg ctctgagctt
    18601 tgcagaaaac tgtaattatt attcggcacc tgacgtaaag ggctgggctg agaaaagatt
    18661 atggtgtacg ggtcagagga ggccatcagg tcttccagcg tgtatttatg gttcaggtaa
    18721 atatcaccca gcacgcagtt ctggacgtag tcctgaaaca gattgatgat ttccggattc
    18781 cgggacagga aatcggtgct tttcacaatc aggttcgccc cgaacagcat gcctgtcttg
    18841 ctgtaggtgg cgctgtccgg ctgagcgaac accatctcat agccggccac catcgcatgc
    18901 ccgatacggg tcgtcagtga aagtggcatc gccagaccga ccggcacatt gtccacccgg
    18961 tgaaccttaa ccaggtcact gttatcaata atctgcaccg atgtgcggac attaaccagc
    19021 aggctgataa gcacaaacac ggccacccag cccagcaaat ccatcacgtt gtgccgttgt
    19081 acccacatca cagccactgc gagaacagat aatgtgagcg caattttttc aatggaatcc
    19141 cacgtccggg tccccataaa agcggcaatg gcgttcaggt tattgcgcaa ccactcacca
    19201 ccggcaatca catagacttc attcacagcg tgctccctcc gaagtgatag ttgttctggt
    19261 aacggctgag catacgggcg gacagctgct ggcgcatgta gctcatctga cggtcaacaa
    19321 ccagcagtgc atcctgctgg acctttacct gcccctggaa aaccgcaatc tgttgctgtg
    19381 cctgattcag attctccagc acactgtcca gcacggtctg tggatagttc ccggtggcaa
    19441 tcatggcccg ggcctgctga agcagttcct gaatgtactg gagcatgatg tcgtagccga
    19501 tatagtccgt cagctggtaa accacgctgc tggacacacc gagcatctgc gggtcaataa
    19561 gatatttaaa gacggggacg gtggtgctgg aaatgaagcc tttttccctg tcatccagcg
    19621 gagtgtcact cacggcttta ttctgaatac tggtgagcag ttttgtaatc tgtgatttca
    19681 gggctttatc ccgggcgatg gtcacatttg catccgccac caccttcaga cacttgtccg
    19741 aatcgttgca gtggtaaatt tttgccgttc ccccctccat aatcgcccgg aggatatcgc
    19801 ggtcagtggt acgcgccggc aggggggtaa tctcgctgtc ttcaccaaaa atcagcgtac
    19861 ctgtgagcgt cataatgaac tctttcagct ctttgttacc atcaagcatc ctgtttttgg
    19921 acagcgagtc ccagatgata tttatgttct tcagcacccg ttccttatct ttgtcactgg
    19981 ccttgtcacg aacagacgat gactgccccc caaccgtgca gccctgacgg gacgcggccc
    20041 agtcggcgaa aatattgctc tcacctgcaa tatcctgaca gaccttctgc tgtgacacct
    20101 gagtctgagg aaataatcct ccgacaatcc cctgtgccac ctgacaggag ctgaggttca
    20161 tactgttaat gtcattcgcc atcttctgca ggaagtcttt cgcggttttg atttcgggca
    20221 ccgttgtctg cagggcaagg tcaaaaaagt accccatcgc gttactcata atctgcttaa
    20281 caaaacgctg cagctgctcg ccattaataa aactgaatga gccgagatag gcatcgatgc
    20341 caccacaccc tgcattgatg tcgggcagtg tcatggagat aagctggata tttctgacct
    20401 gattacgggc atacagagaa ccgccatagg catacccggc agcctgcccc tgccagactc
    20461 cggcctgggt ggtattggtg gcaaagccca gcttgttgaa gaactggttc atgtcgctgt
    20521 tcacatccgc tgacgctgca ggtgtgaccg tcagtaaagc ggcacaaaga accagaagag
    20581 gtttaatgcg aagcattatt gccaccctcc cttaatttgt tatcccggcg catacggcgt
    20641 ttcagccgga atatcctgat ccccccgttt attaattcat ttaaagggtt cctgacgtat
    20701 acgataagaa atccacctgc aaacgcccat aacgtatacg gaatgacata catcaggatg
    20761 ttccacatat agtggaaatt aagtaatttc gggtccgctt tccacgaatc aatacaggca
    20821 tcaaaaatac tgtgcataat cacccataaa aaagttccgg cagcaacgaa cacaaaaaac
    20881 agatcaaaat atatatcaga aaccctgcgt tcataatcat aatcccgttt agtttttctg
    20941 tttttttgtt ccgcatcatt tcttttttct ttcattataa gtttcctctg atatttctga
    21001 tgattcattc agaccgttca tgcggctgta atatatttca gatactgaaa tccatcccac
    21061 aattgccccc acaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt
    21121 tctgcagggc tggcgggata aaaaagtccc tgacggataa aggggatatt caggctgacg
    21181 attattcccg tcgcgacgcc ggatattccg gcaatgagta acaccacctg cttatttcgc
    21241 accttttttc cctccgtaca tctgcagaac ggtatccatc cgcgccataa aaccggcggc
    21301 atccgttgcc cctgtaaaag cggtaatgcc tcaagcgtgt tgacgttgac caggaaggtg
    21361 gtcggtgtgg ccaccgggat attcgggaag aatgttttca gtacatccgg tggtgcaggt
    21421 aacgcctgcg gaaaggccgt atcaccctga ccatccaggg tatagggaaa tacggaaaat
    21481 ccgtactgca gcgccagttg tttcagtacc gggtcaaact ggtgacagta cggacatcgc
    21541 ccttgcatga acagcaccac tttccagtca gcaagattga cctgagttcc gttgctgagg
    21601 cggaaccagc gggttgctgc tttctcctgt cgcacctgtg cgtctgctgc cggctgagtt
    21661 gtccgtgctg ccattccctc cggatgccag agtcgctcga tttccgcacg tgtggatgcc
    21721 tgtgtacagc tggccagaaa aagcaggaca acggacagcg atttaacggg aaacatgttc
    21781 atcatcatta tccttccgtt ctctcagtaa caactgctgt ctgacttcca gtgcctcacg
    21841 ctcttcctga ctgacgcggg ctatccatgt atccagtatc tggtatgcgc caaacagaac
    21901 gagaatggct gcaataagct ctgccagaaa gaaagccatc cagggttttc tgtaaaccag
    21961 acgggcaaca atatggaacc agacacccag ggaaaatacc cacatccccg taatatccag
    22021 tcggggtaaa gagaatctgt gtttacttat catttgaata tcctgttgtg gtttacatga
    22081 atttattcac gtatataacc tgcttaactt tcgtgaaatt aatcagtgtg ttatatgaaa
    22141 aagttatctt tcaaagaaaa actggaaata gtgagaaaca tcataagaga gtcattatca
    22201 ggtaatgcgg cttttgcttt actggtgtat tcattattgc attccttccc cattaaaacc
    22261 tttgctgatt tatttgtgac gatactgtta tttatagcaa tcatcgctgt tgtgatatgg
    22321 ctatttctaa tcatctatgt ttatttttgt gaattattca gaagtcactg gatagcagtc
    22381 tggtttatcc tctggtctgt ggtaatcaac ctcactattg gatttgtcat ttatgacaaa
    22441 ttagcaccag tcatttcact aatgaaataa taaaaaacca cattactcct gaccatgctg
    22501 atgacaacat aatacagaca aatattcttc tgaacctctc gaacttcgtg ataaccggaa
    22561 tggtaccgac tgccattgaa acaacgggta cggcacactg attcataacc gcaccacaaa
    22621 gaaacaagaa attatctggc agcacacata cacttatgac atttactaca aagaagacaa
    22681 acgagctcag cagataaact cctttattat ttggtcctgt aaagaaataa ccaacacccc
    22741 ccacgataaa caggctggca cctgtaaaaa aacacaaatc ccctgtatgc atatatatcc
    22801 tttggttaaa aattcggttt aaaatcttca gatacgttca ggaactgttt tgccaggtca
    22861 tcctgcgaaa taaagccgta tgataatgga cgaacactgc cctgtttcgg gtctaccagc
    22921 atcatggccg ggaaatattt cacgccgagg cgctgcgcct gcccctggtc agtccgggaa
    22981 tccggcaata gtggattaat cacgccatcc acggaaacgg gaataacact cagaccatac
    23041 gtatcccgga agccattaat gacctgcgcc agctgcccgt cgatggggtc ctgcccccgg
    23101 taaaaaaaca taatgccgta atgctctgcc agtctggcaa tcgcctgtcg ctgctgcgcc
    23161 tggtctgctg ccagctggtt ccgtaccgtg ccgttgtaat ggctgtactg caggttatag
    23221 tccagttcag gatgtgccag catggccttt ttggcgctca tggtgaaaag cccggcctgc
    23281 tgagtccagt aattctgcag ccggaaatat ttcacaaaat tatccacgcc gggatacaga
    23341 atggcttcgt acagcgcccg ctttgttgcc gtctgcagcg cggtcagttt ctgcataata
    23401 tccggctcct gacggggagc tgctagtact ggtttatttt ctttttcctt cggatttatt
    23461 ttctcgttat accactgcca gcctgcatct ttccctgacg ccggaaaaat aaagcagcag
    23521 agtaacagcg gcagtaatgc tttattcatc ttcattcctt tcatcacttt ttctttcatc
    23581 aggtttctgc aggtcagcca gtgcagaaaa acaaccatac agaataaaca ggagcacacc
    23641 ggagtaaagg ataatgaaag cagcagcaag caccccgcct gccgtggttt cttttaccgt
    23701 atcccacgac cagataatta ccggggaaat gacaattgct gtaataacaa gccggataag
    23761 aaaatcaaca agacgattcg acgtgaatat gactttcatt attttcccga ccttactgcc
    23821 ccacctgttt cagctgaccg gcaatctgct ctttcacttt ttcggtcagc gcaccattat
    23881 ccgggatttt ctggttattc atcaggtctt cgtagaagtt agtgaagtcc agctggtcga
    23941 acttaatacg ctgcagttca tccacggtta ttccccggca gtcaggactt tttgcgctgc
    24001 caaacccgat acgcaactgc ccgttacgcc cctgctgctg gactatctgc gccagcttcg
    24061 aatcaaactg gcagtaactg cgctttttct gcagacagac acccagcact ttccgggagc
    24121 agaactcgcc cacactcacc gtcagtttgt tgtcctttgc ctttcccagc gccttttctt
    24181 cgctgttgca cttcgccaaa cccacatcct gaccccagcc accatcctta cagcagttac
    24241 tgaagccggc tgcggcctta cggcagaatt tcgcctcgcc cgtgaaagcc ctgacatcca
    24301 caccattcag ggcggcaaca tccttacctg cggcggcaag cgcagccagc tgtgatactg
    24361 cctcaccaaa atcactgctt tttccgctct gcgccttgtc gcattcaccg tcaaggcaga
    24421 agacatcacc accacagacc atcacttttc cggacgttct ggactcgcag gagtacgtgg
    24481 catactcatg cagacaagct ccctcatcgg aataaaacgc gcactgacgt gaggccagcg
    24541 tacaggccgg attatcgaca tatttctgac aggtgccgtt atctgcggac tgtgtgacat
    24601 atttatcccg gtatgcccag cacgcctgtg tgatgctcca ggttttacct tccatcacac
    24661 ccgttttcgt accacccggt tctgaacact cagtgccttt cagcacgcca tccgctttat
    24721 taaacggaca actctccacc cactccaccc gtggaaccca ttccttatca cggaccttca
    24781 tcctgagttt cagcgtaaag gtggaagcac cactgacaag cgattcatag accatcctgt
    24841 caccattccc gtgcgggagg caattaccgt ttgcagtaca gccactaccg gtcagaacct
    24901 gcccctgtgt gacagaaaac ccggagggca caggtatggt gaaagtcccg ctctgtcctg
    24961 taaccagttg cacattaaag gctgtgttca taaagtcgta acgggagtta aggaaatata
    25021 gccctgcatg agccgacagc gaggcacttt ccactgtccc ggcaacaggt acggtaacgc
    25081 tgaacaccag tttcagaccg ttcatactga accggaactg tgagtgcggt atcgtcacct
    25141 cacgatattc atccgtgtat ttccagtctc ctgtgatcct ggctgtcctg gaacaatatt
    25201 cctccaccat cgtgtcacgt tcacaggtat agtttgtgaa ttcactgcgg tttatctgct
    25261 gcgcactgca ctgctgtccg gtatttccca cgatactgtc agcccggttc accacatccc
    25321 tgccgatctg aataaacggc gcatccggtg acagaatgtc tttcggcttg ttcatgaatg
    25381 actctgtgat ggttttgccg gtttcactgg ttgcccattg tgtggtgccg ttatttttca
    25441 gaccgccatc cccgccggca gtcaccccac cgtaatactg cgtctcatcc gggttcgcgt
    25501 tatacccggg gatactctcc tgcggattaa aactctgaat actgccaata ccctgcccct
    25561 ggatctgacg ggcaaaatcg gaacctgccc ggtaatcact gttgctgtcg gcaaatacag
    25621 aaaagctcag gaaaaaaaca attgcacaaa tattgcgcgg tcttccggag tcattaacat
    25681 tcagagacct gaaaaaggta tttatctttt ctttcataac ctgatccata ctggagaatg
    25741 aagtgatgtc atctcaactt cagaacggtg ttgattttgg tgtatggagc ttcctggcgt
    25801 accgggatta ctactcagat aacgacttca gtaacaatat cagcgtcctg aagtctgcac
    25861 gcagtgaacg acgacagtac cggtgatacc agcaatgagg accttccggc tgaagccgga
    25921 aacagagctg aaggtcactg ttaaaagccc ccttcaccga ccatccccat gtcagacgtg
    25981 gtattgctga ttcagcatcc tgccgcagtc gctgctcaag aagggcagtc atttttaccg
    26041 gacaggcaaa ggcaatataa ggcagcagct ttgtgaccgg gaaaccatgt ccctgacagg
    26101 aggcatatgt acggaatccg tgaacgttca tcgcggccac cagtgcacgg atgtccggtt
    26161 caatccggta atacccggca ggtaaactca ttgcagctta tccccggtgc cgtccagcag
    26221 tccctgagcc acctgccggc agtctcccgt ggcggccact ttttccagcg cctgcctgac
    26281 gcgcagattt ccccggatga tgtcgtgccc ctggctgcag aacaccacca gggccggcac
    26341 gctgcggatg ccgtactgtg aaaacaacgt cgggtcgatc tgaacaccat cggtcgcgcc
    26401 atcttttacc agggacaaca cggcttcagc ggtagtcttc aggtcattgt tcaccatacc
    26461 ccgcagtgtg gccggaatgc cgtagtgcct ggtttctccc agcatccgtt tcagtccctc
    26521 ttcgggaatg gagaacgaca caaaatacac ggccccctgc tgtggtttgt ctttctgagc
    26581 agcctgctgt ttacggacca gttcatcgag aaactggtta tcggcatgct gaaggggatt
    26641 cgccctgacc tgctgttctg cccatgcctg cagttgtgtg tccggctttt cacgcaactg
    26701 ctgactcagt gtttcctgct gcttcaggaa ctggcgattt tcgggcgtat tcacattttc
    26761 cgaagcatgg gcagaaagaa acaatcctgc tgtcagggcg aataaaatgt atattttttt
    26821 gttcatataa ttctcctgca aacagacagt aattaatcgt cataatgagg tgctcaatag
    26881 agcaagcctg gagataactg acgccatgtt aaacacaacg aagatcgttg attaacttct
    26941 ttctccattt atcttttttc tgagactggc agcaattaga taaacgatac cggatattca
    27001 ctctgttaaa tcattaattc atttctgacc gatgcacata aaatgagtca gatattaccc
    27061 gttattctct tcttcatttt tgtttaatct ctttcccggc agaaactcat cagagcagag
    27121 aaactccaaa aaaacagccg cccaggtgca taaaaaagtt gttcccagat atagtctgat
    27181 aactctgatc ctgtctgaca catcacttct ctgccattca ctgataatgt cacagataac
    27241 attttcaggc ataaaataaa acaacaccgt gaatagcatc accagtgcaa aaggaaacaa
    27301 ttttaatgct ttgcctgtca aacgaatgaa cacacaggat atactcatat aaatatgaat
    27361 gtatttactg cttaatttct tttccattta aaatattcct ctttttgttt aagttacaga
    27421 aatacacagt tacgtttacg ccacatcagg taaccgaagt ttttctttgt gtcaggcgga
    27481 tttttttccg tctcccggcg catcacactg cgcccgaacg gatggcactg tccgctgtcc
    27541 gctgtccgct gtccgggtac atgttcacca ccaggtgttt ttaacagata atcagaaaaa
    27601 acagcacagc acacaaaata aacacaatta cacactcaag gaaaatccac aggtatttac
    27661 gttcagcact ggcatgcagc ctgataatac aaccatcaca gtcataccgt gtcacaaact
    27721 gcaggtattt ccgtatatct ttcagaacat caattaacga ctgaatttca actgcaagga
    27781 atgactgacg cccatcttcc cgcactaccc ggaacttctc atcccgcaat aaaaatatta
    27841 taaaaccaac ctgtccgggt gatggataat agtgatgatg taacatccat tgaatatctg
    27901 caattccacg ctgaatatca tctttttcac atagcctggt gagcggccgg gtattcatcc
    27961 gatgaaacac cgccaaaagg gcctgtttga cataagcaaa atcatttact cttgtccctt
    28021 cactcattta atatcaccct taaacatctg ttcatgtatc agtttcggag tgcaaatggt
    28081 cataatactg atattcttat cgctgttgtt aacggtcctg cttcgttttc gcttcataaa
    28141 taacagtctc tttttcagag ttctgagaag accattcccg gctggcttta ttttgttttt
    28201 actgtcatcc atcatacatc tgatgataca aaaagattat tcacctttca gcggaaacat
    28261 atactctgta attatcaggt cagggatatc tgtaattaca ctatggtttt ttctgttctt
    28321 tttctcatcg tccatgctca gaacgcctgt tacgggtctt tatcttaccc cctttatatc
    28381 cagcctgaga atcgatatac atccacaata tattaccaga ccatctgtca aagtatcctc
    28441 aagaatcaac gcagacaagc ttgtcggaaa tattgaacat ttcatattat gctatccgtc
    28501 ttataacacg atcacattac agtctcacat cctgcgccac ggaatccgaa acagaatagc
    28561 tatgaggtag cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag
    28621 ctaataatga gcagaaaaac ccaacgttac tctaaagagt tcaaagccga agctgtcaga
    28681 acggttcttg aaaatcaact ttcgatcagt gaaggcgctt cccgattatc tcttcctgaa
    28741 ggcactttag gacaatgggt taccgccgcc agaaaagggc tcga
//
LOCUS       DAZGCQ010000060        28576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000060 DAZGCQ010000000
VERSION     DAZGCQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.060"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..598
                     /gene="cmtB"
                     /locus_tag="ACHK07_002901"
     CDS             155..598
                     /gene="cmtB"
                     /locus_tag="ACHK07_002901"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO8103207.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSATDADSHIGAIQALSELLCEEEILEQLLTASSEKKLADIISRG"
     gene            626..2014
                     /gene="cmtA"
                     /locus_tag="ACHK07_002902"
     CDS             626..2014
                     /gene="cmtA"
                     /locus_tag="ACHK07_002902"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO8103208.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2029..3306
                     /gene="yggP"
                     /locus_tag="ACHK07_002903"
     CDS             2029..3306
                     /gene="yggP"
                     /locus_tag="ACHK07_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8103209.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3303..4268
                     /gene="glpX"
                     /locus_tag="ACHK07_002904"
     CDS             3303..4268
                     /gene="glpX"
                     /locus_tag="ACHK07_002904"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8103210.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVEVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4290..4799
                     /gene="fumE"
                     /locus_tag="ACHK07_002905"
     CDS             4290..4799
                     /gene="fumE"
                     /locus_tag="ACHK07_002905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO8103211.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMNTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4796..5509
                     /locus_tag="ACHK07_002906"
     CDS             4796..5509
                     /locus_tag="ACHK07_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO8103212.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5481..6158)
                     /locus_tag="ACHK07_002907"
     CDS             complement(5481..6158)
                     /locus_tag="ACHK07_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8103213.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     QPDDIHP"
     gene            complement(6152..6829)
                     /locus_tag="ACHK07_002908"
     CDS             complement(6152..6829)
                     /locus_tag="ACHK07_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8103214.1"
                     /translation="MVTLEQFRYCPTHTTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPHASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6817..7523)
                     /locus_tag="ACHK07_002909"
                     /pseudo
     CDS             complement(6817..7523)
                     /locus_tag="ACHK07_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(7524..8102)
                     /locus_tag="ACHK07_002910"
     CDS             complement(7524..8102)
                     /locus_tag="ACHK07_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO8103215.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPICTTRHFPAMAAVR"
     gene            complement(8125..8556)
                     /locus_tag="ACHK07_002911"
     CDS             complement(8125..8556)
                     /locus_tag="ACHK07_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO8103216.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8928..9947
                     /gene="epd"
                     /locus_tag="ACHK07_002912"
     CDS             8928..9947
                     /gene="epd"
                     /locus_tag="ACHK07_002912"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO8103217.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRSEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9997..11160
                     /gene="pgk"
                     /locus_tag="ACHK07_002913"
     CDS             9997..11160
                     /gene="pgk"
                     /locus_tag="ACHK07_002913"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO8103218.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11375..12454
                     /gene="fbaA"
                     /locus_tag="ACHK07_002914"
     CDS             11375..12454
                     /gene="fbaA"
                     /locus_tag="ACHK07_002914"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8103219.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12518..12616)
                     /locus_tag="ACHK07_002915"
                     /pseudo
     CDS             complement(12518..12616)
                     /locus_tag="ACHK07_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12812..13672
                     /gene="mscS"
                     /locus_tag="ACHK07_002916"
     CDS             12812..13672
                     /gene="mscS"
                     /locus_tag="ACHK07_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8103220.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13811..14446
                     /gene="argO"
                     /locus_tag="ACHK07_002917"
     CDS             13811..14446
                     /gene="argO"
                     /locus_tag="ACHK07_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO8103221.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14539..15279
                     /gene="yggE"
                     /locus_tag="ACHK07_002918"
     CDS             14539..15279
                     /gene="yggE"
                     /locus_tag="ACHK07_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO8103222.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15447..16343
                     /gene="ygfI"
                     /locus_tag="ACHK07_002919"
     CDS             15447..16343
                     /gene="ygfI"
                     /locus_tag="ACHK07_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8103223.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16340..17818)
                     /gene="scpC"
                     /locus_tag="ACHK07_002920"
     CDS             complement(16340..17818)
                     /gene="scpC"
                     /locus_tag="ACHK07_002920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO8103224.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17842..18627)
                     /gene="scpB"
                     /locus_tag="ACHK07_002921"
     CDS             complement(17842..18627)
                     /gene="scpB"
                     /locus_tag="ACHK07_002921"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO8103225.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18638..19633)
                     /gene="meaB"
                     /locus_tag="ACHK07_002922"
     CDS             complement(18638..19633)
                     /gene="meaB"
                     /locus_tag="ACHK07_002922"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO8103226.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19626..21770)
                     /gene="scpA"
                     /locus_tag="ACHK07_002923"
     CDS             complement(19626..21770)
                     /gene="scpA"
                     /locus_tag="ACHK07_002923"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO8103227.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARVIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21974..22867)
                     /gene="argP"
                     /locus_tag="ACHK07_002924"
     CDS             complement(21974..22867)
                     /gene="argP"
                     /locus_tag="ACHK07_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO8103228.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(22938..24263)
                     /locus_tag="ACHK07_002925"
     CDS             complement(22938..24263)
                     /locus_tag="ACHK07_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFO8103229.1"
                     /translation="MKKANLTGTLVCFVACIIFLSSSDIFREKPIALIWFIRQNTLFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLAGECAMFIMIAMVFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFVAFISLLKLNGNK
                     SKHQATVKSIVNHPGQITEVILEMEHPIDYCPGQFAFLTFAHSKESHPFTIASYVKEK
                     NTLRFAIKNLGDYTSTLASSIQIGQSALVEGPWGKFDFTLPCSRQVWIAGGIGITPFI
                     AQLEYLNQHGASRVPVDLWYCVSRSEDAWYVDKLTSLCAQASVTLHLLDAHKGERLQS
                     HYLTDKIANKGDTHFWFCGPQSFAKALSKGLYENGIASQNFHYDRFCMR"
     gene            complement(24325..24585)
                     /locus_tag="ACHK07_002926"
     CDS             complement(24325..24585)
                     /locus_tag="ACHK07_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO8103230.1"
                     /translation="MSIKQNLFAALFLCTLTGHAFAATEIHKPDNNLEQLSKVSMSTH
                     NSNPAGVESQIKEKAEELQASNYYISSFSGKNYIHATVIFYK"
     gene            complement(24602..25231)
                     /locus_tag="ACHK07_002927"
     CDS             complement(24602..25231)
                     /locus_tag="ACHK07_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFO8103231.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQTLVEALCQHFPKATIIHRDVGL
                     TPPPHLREETFMALCDKAECGSAQIRQEVNGIHAAIEELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPELITQVFNHYL"
     gene            25332..26228
                     /locus_tag="ACHK07_002928"
     CDS             25332..26228
                     /locus_tag="ACHK07_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8103232.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALMAQEERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIEDLNHVDGILRLANNGGSQWQLINGEQEITLQPEA
                     RLQSNNLRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            26284..26943
                     /gene="rpiA"
                     /locus_tag="ACHK07_002929"
     CDS             26284..26943
                     /gene="rpiA"
                     /locus_tag="ACHK07_002929"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO8103233.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            27199..28431
                     /gene="serA"
                     /locus_tag="ACHK07_002930"
     CDS             27199..28431
                     /gene="serA"
                     /locus_tag="ACHK07_002930"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO8103234.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
BASE COUNT         6996 a         6958 c         7565 g         7057 t
ORIGIN      
        1 taaaaatatc aatttcatat tgttgttatt taaggtttta ttattttggt ttatggggtt
       61 tttcttctcg cgtacccaat tgctttcatt aaaccgcacg agtattttat ttttaccgcc
      121 ggacggtacc ctaaaaataa aataaggata atttatgcgg cttagtgatt attttccaga
      181 atcatcaatc tcagttatac attcagcaaa agattggcag gaagctatcg atttctcgat
      241 ggtatcattg ctggataaaa actatatcag cgagaattac attcaggcaa ttaaagattc
      301 caccattaac aatggccctt attatattct cgcaccaggc gtggcaatgc ctcatgcgcg
      361 accggaatgt ggggcgctta aaaccgggat gtctttgaca ttacttgaac aaggtgttta
      421 ttttccgggg aatgatgaac caattaaatt actcatcgga ctctctgcta ccgatgccga
      481 ttcgcacatt ggcgctattc aggcgttaag tgaattactg tgcgaagaag aaatactcga
      541 acaactctta acagcatcat cagaaaaaaa attagcggac attatcagcc gcggataatt
      601 actctctcct ctacaggata atattatgga aaacaagtct gctcgtgcaa aggtccaggc
      661 ttttgggggc tttttgactg caatggtcat ccccaatatt ggtgctttta ttgcctgggg
      721 ttttattact gcgttattta ttcccaccgg ttggctgcct aacgaacatt tcgccaaaat
      781 tgtcggcccg atgattacct atttattgcc cgtgatgatt ggttctaccg gtggtcatct
      841 ggtcggcggt aaacgcggcg cggtcatggg cggaataggt actattggtg tgatcgtcgg
      901 cgcagagatc ccgatgttcc ttggctcaat gattatgggg ccgctcggtg gtctggtcat
      961 aaaatatgtt gataaggcac tggaaaaacg catacctgcc ggttttgaga tggttatcaa
     1021 taacttctca ttaggtatcg cggggatgct cctttgcctg ctgggttttg aagtgatcgg
     1081 cccggcggtg ttaattgcca atactttcgt caaagagtgt attgaggcgc tggtacatgc
     1141 gggttatctg cctctgctgt cagtcatcaa tgaaccggcg aaagtgcttt tccttaataa
     1201 tgcgatcgat cagggcgtct attatccgct gggaatgcaa caggcttcgg ttaacggtaa
     1261 atccatcttc tttatggtgg cctctaaccc aggtccgggc ctggggctgc tgctggcgtt
     1321 taccttgttt ggtaaaggga tgagtaaacg ttctgcgccc ggggcgatga ttattcactt
     1381 cctcggtggg atccacgaac tgtatttccc atatgtgctg atgaagccgc tgactattat
     1441 tgccatgatt gcgggcggta tgtctggcac ctggatgttt aacttactgg acggtggtct
     1501 ggtggctggc ccaagtccgg ggtctatctt tgcttacctg gcgctgacgc cgaaaggttc
     1561 gttcctggcg acaattgccg gtgttacggt aggtaccctg gtgtcctttg ctatcacttc
     1621 gctgatactg aagatggaaa aaacggtgga aacggagagc gaagatgagt ttgctcagtc
     1681 agccaatgcg gttaaggcga tgaaacaaga gggtgcattc tcgttaagca gggttaagcg
     1741 tatcgccttt gtttgcgatg cggggatggg ctccagtgcg atgggcgcga ccaccttccg
     1801 taaacgcctg gaaaaagcgg ggctggcaat tgaagtaaaa cattacgcca tagaaaacgt
     1861 gcctgcggat gcggatatcg tcgttactca tgccagtctg gaagggcgcg tgaaacgtgt
     1921 gacggataaa ccactgatat tgattaataa ctatattggc gatccaaaac tcgacacttt
     1981 atttaatcaa ttaaccgccg aacataaaca ctgattacag aggtaaaaat gaaaaccaaa
     2041 gttgctgcta tttatggcaa gcgggatgtc cgtctgcgcg aatttgaact gccagaaatt
     2101 accgataatg aattactggt gagtgtgatt tctgacagcg tctgtttatc cacctggaaa
     2161 gcggcgttac tcggtagtga acataaacgc gtacccgacg atttagaaaa tcatccggtc
     2221 attaccgggc atgaatgtgc cggggttatt gtcgaagtgg gtaaaaatct cactggaaaa
     2281 tataaaaaag gccagcgttt tgtattgcaa ccggcgatgg ggttaccaag cggatattca
     2341 gcgggctaca gctacgaata ttttggcggc aatgccactt atatgattat tcccgaaatc
     2401 gccattaatt tgggctgcgt attaccgtat cacggctctt attttgctgc ggcgtcgctg
     2461 gcagagccta tgtgctgcat tattggtgct tatcatgcca attatcacac cacgcaatat
     2521 gtttatgagc atcgcatggg cgtcaaacct ggcggcaata ttgcactgct ggcgtgtgca
     2581 ggtccgatgg gcattggcgc tatcgattac gccattaacg gcggcataca accgtcgcgg
     2641 gtggtggtgg tcgatatcga cgacaaacgt ctggcgcagg tacagaagct gctgccggtg
     2701 gaactggcgg ccagtaaagg cattgagctg gtgtatgtga ataccaaagg gatgagcgat
     2761 cctgtccaga tgctgcgggc gctgacagga gatgccgggt tcgatgacat ttttgtttat
     2821 gcggcggtgc ctgctgtcgt tgagatggct gatgaattac tggcggaaga tggctgtctg
     2881 aacttctttg ccgggccgac ggataaaaac ttcaaagtgc cgtttaattt ctacaacgtt
     2941 cattacaaca gcacgcacgt cgtcggtaca tccggcggtt caacggacga catgaaagag
     3001 gcgattgccc ttagcgccac tgggcagtta cagccgtcgt ttatggtgac ccatatcggt
     3061 ggcctggatg cggtgccaga aaccgtgctc aatctgccgg atatccctgg cggtaaaaaa
     3121 ctcatttata acggcgtgac catgccgctc actgccattg ccgattttgc cgaaaaaggc
     3181 aaaaccgatc cgctgtttaa agagttggcg cggctggttg aggaaacgca cggcatctgg
     3241 aatgaacagg ccgagaaata tctgctggca caatttggcg ttgatattgg ggaggccgcg
     3301 caatgatgtc cctggcgtgg ccattatttc gcgttacgga acaggcagcg ttggctgcct
     3361 ggccgcaaac cggatgtggc gacaaaaata aaattgatgg cctggcggtc accgcgatgc
     3421 gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt tatcggcgaa ggagaaattg
     3481 accatgcgcc gatgctgtgg attggcgaag aggtgggcaa aggcgatggg ccagaagtcg
     3541 atatcgcggt tgatcccatt gaaggcacgc ggatggtggc gatggggcag agcaatgcgc
     3601 tggcggtcat ggctttcgcc cctcgagata gcctgttaca tgcgcccgat atgtatatga
     3661 aaaagctggt tgttaatcga ctggctgccg gggcgattga tctgtctttg cctctggcgg
     3721 acaacttgcg taacgtggcg cgggcgttag gtaaaccgct ggacaagctg cgcatggtta
     3781 cgctggataa accgcgtctg agtgccgcga ttgaggaagc aacacagctg ggggtgaaag
     3841 tttttgccct gccggatggc gatgtcgccg ccagcgtgct gacctgctgg caggataatc
     3901 cttacgatgt gatgtacacc atcggcggtg cgccggaagg cgtgatttct gcctgtgcag
     3961 tcaaagcgtt aggcggagat atgcaggcgg aattaatcga tttttgtcag gctaaagggg
     4021 attacacgga aaatcggcag attgcagagc aggagcgcaa gcgttgcaag gcaatgggcg
     4081 ttgaagtgaa ccgtgtgtac tcgctcgacg aactggtgag ggggaacgat atcctcttta
     4141 gcgctacggg cgtcacgggc ggcgagctgg tgaacggtat ccaacagacg gcgaatgggg
     4201 tgcggacgca gacattactg atcggcggcg cggaccaaac gtgtaatata atagactccc
     4261 tgcattgatg gtgatctatc ggaagaccaa tggcgacgct gacagaagat gatgtgcttg
     4321 agcaactgga tgcacaggac aatttatttt catttatgaa tactgcgcat tctattttgc
     4381 tccaggggat acgccagttt ctgccgtcgc tgtttgtcga taacgatgaa gagatcgtcg
     4441 aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct tgacgatatt gatgttgcac
     4501 tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct gtacgccgat atcacgcatt
     4561 tttcccagta ctggcattac ctgaaagaac aggatgaaac gctcggattt gccgatgaca
     4621 tgacttggga ttttatcagc aacgtcaata gcataacctg caatgcgacg ctctatgacg
     4681 cgttgaaagc gatgaagttc gccgatttcg ccgtctggtc agaggcgcgt tttagcggga
     4741 tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt aaaggaatta acgccgtgaa
     4801 aattgaatta acggtgaatg ggctgaacgt gcaagcgcag taccatgatg aagagataaa
     4861 acgcgtgcat aaaccgttgc tgcgtatgct ggcagcctta cagacggtaa atccgcagcg
     4921 gcgtactgtg gtttttcttt gcgctccgcc ggggacgggg aaatctaccc taaccacctt
     4981 ctgggaatat ctcgcgcaac aagacccgga actgcccgct attcagacgc tgccgatgga
     5041 tggttttcac cattacaata gctggctgga tgcgcatcaa ttgcgcccct tcaaaggcgc
     5101 accagagaca tttgacgtag cgaaactggc ggaaaatctg cgccaggtcg tggaagggga
     5161 ttgtacgtgg ccgcagtatg atcgacaaaa gcatgatcct gttgaagatg cgttgcacgt
     5221 taccgcacca ctcgtcatcg tcgaaggaaa ctggttgttg ctggacgacg agaagtggtg
     5281 ccagcttgcg caattttgtg atttctcgat ctttatcaat gcgccagcta ctgcgttgcg
     5341 ggaacgactg gtggggcgca agctggcggg agggttatcg ctggcagacg cagaagcttt
     5401 ttatgaccgt accgacgggc cgaatgttcg acgggtgctg gaagagagtc tgcctgcaaa
     5461 tttgacctta atgatgaccg ctacgggtga atatcgtctg gttgattaac gttcctgatt
     5521 agaccatctt ccagtcgtac cacacgagag aaatgacgcc gggtaaatgc ggcgtcatga
     5581 ctgattgcta cgacgctcgt gcctcgctgg cggcacttct ccagccagct ttcaaacacg
     5641 ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca gcagcaatag tggcggagag
     5701 agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg cactatgcag atccaatggg
     5761 tgcgcgctgg cagtgtctgc taactgacaa cattgcaggg cagcatgggt acgttgagtg
     5821 atttcatccg ccgggcattt ttgcagtttc aaaccaaatg cgatctcgtc agcgacggtg
     5881 ctgtgaaaaa gttggttttc cgcttcctga aacagtacgc caatctttgc cgcgcgctgg
     5941 cggtttttca ggtttttcat cgcctgttgt tgcagcataa ccgtaccggc tgtgggcgta
     6001 agaagacctg ccattactcg taacagcgtc gatttccctg cgccattgtc accggtcagc
     6061 gccaaccatt caccttgttt gagttgcaga gaaatgtcgc aaagacaatc cgttgccgcg
     6121 ccaggccagc gataagatat ctgatttaac gttaacatag tggctggagc gctccgtttt
     6181 gcaattgcca cgcttgctgg cagtagttca ggaaaggtgt gcgatgacgt tcaaaaagaa
     6241 taatcagcga accacgttca aacgcccagt gttgtaagcg ctgcaacagc atttcgctgg
     6301 catgcggcgt caggcggcta aaggcttcat caagaattaa tagctttggc tgcatcgcga
     6361 tggcgcaggc gatgaccacg cgctgtgtct ctccaccgga gagtgttgct gggtgacgat
     6421 ggcgcaacgg ctggcactcg gtcagagtca gggcggcatc aatccgcgcc ataatctctg
     6481 cttcagcaag acacagattt tctggcccaa aggcaacttc ttcttccacg ctaaaggtac
     6541 agccggagag ctgcaaatag ggcgattgct ggacgagctg gatggtggcg gattgttcat
     6601 taagcgggag atgcccaatg ggcgtaccga ggagcgtccc cgtgcctgtt atctctcctg
     6661 gcagaaagtc cggataccag cccgccatca gctgtgccag cgtacttttg ccactgccgt
     6721 tgtcaccaaa gatggcgacc atccccggtt tgacatagtg aaagtcataa cagaacggag
     6781 gatgcgttgt gtgtgtcggg caataacgga actgttctaa tgtaaccata tccacgctcc
     6841 gaattctgtc agcatcagca agatcatggt gtaacgcgct acgcgttgta gggtgctatc
     6901 ggctggtgcc cacaacgttg ttcgtcggtt gtggatccga aatgcccgca tatccagcgc
     6961 cgcaccacgg acagcaagat cgtttagcgc attgtgtgtt agcggaataa ttaacgcggg
     7021 catcgcacgt aaacgctgat accagccctc atccagcggc acgccgcggg cacgctgcgc
     7081 ttcatgaata atcgctaatt gccgttttaa ctgctcaacg accaataacg gcccggcaaa
     7141 cagataagcg acgcctggcg gcaggcgaga agcaaacaga gcgcggataa aacattgcac
     7201 ggggacgtac tgcatccaca actgtgaggt tgaaacaatc gccagaatcc gcaaccagag
     7261 cgtaatagca tgcgtccaac gctccggcga acgcggcgtg ccgcttagcc attctgttag
     7321 ccagccaccg tgtaccagcc acagcccggc tcccagcgag aacatcagcc agacgacata
     7381 ttttgcccgc cgccgcgtgg cctttagcgc aataaggcaa aaaaggtcgc cgcgctatag
     7441 atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc aggccgccag cgcccacagc
     7501 gttaaggatg taaacggatg catttagcgc acggcagcca ttgccggaaa atggcgcgtg
     7561 gtgcaaatcg gcagctggcg cagtaaaagc cagacaatca ccgctgtaag aattttatcg
     7621 accaggtttg cgcctatcac cgtaatcgcc accgactcca ccaggttttg ccccatagag
     7681 tgcatccagg caacgaacaa atcggctccg ctaccggtga caccgccgaa cagcgcagta
     7741 cgcagcggta cagcgacgac cgttaccgca agcgtaataa tcacgccgct gacaacgact
     7801 tttggcaggg tgcggaacca acccgctcgc gccagccaac cggcgaccag gccgatgacc
     7861 atcgccacgg gtgcaaatgc ggcggcgatc gggtcggtca gcaatcccca gagtaaattg
     7921 gtcagtaagc cggtcagcat tccgatcacc ggaccaagca gtaccgcgct aataagtgtg
     7981 ccgatggagt cgagaaatat aggtaatttg accatgctgg ccagttgccc gccgatcata
     8041 ttgatggcga tagatataac gatcaacacc agtgcctgac tggaaaagtg gctacgcgcc
     8101 ataagaaaac ctctctgaaa gtagttattt aacgggggaa atatgcaact cgtcgtaacc
     8161 agaaagcgcg caaggttggc ggctaaacgc cagttcctca aggctaccga tcaccagttc
     8221 aagcgtggtc cgcacggtac agccgggcat catatgaccg ccaagcatcg tgccgtgtgg
     8281 atcggaaacg cagagatgaa gatgctcacc actctgttcc aacgtgccgt ttagtgagat
     8341 cacttcgaat gtaccgttca ggagcgtggt gccttcttgc ccggcatagc gcaaggcgat
     8401 gtcagtcagg ctaccggtac agcctgcaat ccaggcggca tgaagttgct gctgctgtgc
     8461 aaaagcgtgt agctgggaaa aaacttcctg cccggggagg aggcgcagtg cgtaaaagcg
     8521 tgcggaggaa gaatatgggt agggcaatgt catcattgga gtactccgaa actcggacgt
     8581 attgcggata atatcgacgc tgcatcagtc atattctgat ataagtcagg gtttgtgacg
     8641 gcacaacgaa aagattttgc tacgcttatt cgtgattctg caaagtggaa taaattccac
     8701 aggggcgggg ctgaaaacgt gacgtaagtc agataattgt tttctttgac tggacaatca
     8761 ttccttttat tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg
     8821 acaattaacc aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga
     8881 ttactctgtc tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg
     8941 tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg
     9001 gacgccgggc ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg
     9061 cgcatttgtt gaaatacgac accagccatg gccgttttgc atgggaagta cgacaggaac
     9121 gcgatcaact ttttgttggt gatgacgcca tccgcgtatt gcatgaacgt tcactgcaat
     9181 cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct
     9241 cccgcgagca tggcgaagcg cacattgccg ccggggctaa aaaagtgctc ttttcacatc
     9301 ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtt
     9361 cggaacaccg catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca
     9421 aattgttaga tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca
     9481 tgcacgatca acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggcagcca
     9541 gccagtcgat cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc
     9601 aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa
     9661 tcgatttaag cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc
     9721 aaaaagcagc acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct
     9781 ctgtagattt taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtca
     9841 gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc
     9901 gaatgctcga cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg
     9961 tctgcaaaac ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat
    10021 ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac
    10081 gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga actggccctg
    10141 aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac
    10201 aacgaagaat tctctcttct gccggttgtt aactacctga aagacaaact gtctaacccg
    10261 gttcgtctgg ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt
    10321 ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa
    10381 tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag
    10441 gcttctactc acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca
    10501 gctgaactcg acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc
    10561 gttggtggtt ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct
    10621 gaccagctga ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat
    10681 gtgggtaaat ccctgtacga agctgacctg gttgacgaag caaaacgtct gctgaccacc
    10741 tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg agttctctga aaccgctccg
    10801 gctaccctga aatctgttaa cgacgtgaaa gctgacgagc agatcctgga tatcggtgat
    10861 gcttccgctc aggaactggc tgaaatcctg aagaatgcga aaaccattct gtggaacggt
    10921 ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct
    10981 atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac
    11041 ctgttcggca ttgctgacaa aatctcctac atctccactg gcggcggcgc attcctcgaa
    11101 ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa
    11161 aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg cttttagagc acgcattttt
    11221 tcatactcta aataattcga gttgcaggaa ggcgacaaga gagtgaatcc ccaggagctt
    11281 acataagtaa gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga
    11341 cgagtataag gcccgacgat acaggacaag agacatgtct aagatttttg atttcgtaaa
    11401 acctggcgta atcactggtg atgacgtaca gaaagttttc caggtagcaa aagaaaacaa
    11461 cttcgcactg ccagcagtaa actgcgtcgg tactgactcc atcaacgccg tactggaaac
    11521 cgctgctaaa gttaaagcgc cggttatcgt tcagttctcc aacggtggtg cttcctttat
    11581 cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct gctatcctgg gcgcgatctc
    11641 tggtgcgcat cacgttcacc agatggctga acattatggt gttccggtta tcctgcacac
    11701 tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt ctgctggacg cgggtgaaaa
    11761 acacttcgct gctaccggca agccgctgtt ctcttctcac atgatcgacc tgtctgaaga
    11821 atctctgcaa gagaacatcg aaatctgctc taaatacctg gagcgcatgt ccaaaatcgg
    11881 catgactctg gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg tggacaacag
    11941 tcacatggac gcttctgcac tgtacaccca gccggaagac gttgattacg catacaccga
    12001 gctgagcaaa atcagcccgc gtttcaccat cgcagcgtcc ttcggtaacg tacacggtgt
    12061 ttacaagccg ggtaacgtag ttctgactcc gaccatcctg cgtgattctc aggaatatgt
    12121 ttctaagaaa cacaacctgc cgcacaacag cctgaacttc gtattccacg gtggttccgg
    12181 ttctactgct caggaaatca aagactccgt aagctacggc gtagtgaaaa tgaacatcga
    12241 taccgatacc caatgggcaa cctgggaagg cgttctgaac tactacaaag cgaacgaagc
    12301 ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat cagccgaaca agaaatacta
    12361 cgatccgcgc gtatggctgc gtgccggtca gacttcgatg atcgctcgtc tggaaaaagc
    12421 attccaggaa ctgaacgcga tcgacgttct gtaagtcatt cctttctgct tatctcaagg
    12481 cccgctctgc gggtcttttt ttcgccaaaa gaaaaattgc ctgatgcgct acgcttatca
    12541 ggcctacacg tatcctgcaa tatattgaat tcgcaggttt ttgtaggccg gataaggcgt
    12601 tcacgccgca tccggcatga caaagcgcac attgatatct gtctgagccc cgctctgcgg
    12661 ggattttttc gccaaaagca aggtgataca gatgagaaat ctgtgatcta tttggcaaaa
    12721 ttatgcttta ttgtttaccc ttgtcagact gcccgtcata aggcggcgga gtgtatttct
    12781 ccattttgag tcagttgaaa aggaatattg aatggaagat ttgaatgttg tcgatagcat
    12841 aaacggcgcg ggaagctggc tggtggctaa ccaggcgctg ctgctaagtt atgcagtaaa
    12901 catcgtggcg gcactcgcga tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa
    12961 cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc actgttgctg attttctttc
    13021 tgcattagtc cgttacggta ttatcgcctt tacgctaatc gctgcactgg gacgcgtggg
    13081 tgtacaaacc gcgtccgtca ttgctgtact cggtgccgca ggcttagctg ttggtctggc
    13141 tttgcagggg tcactgtcta acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt
    13201 ccgtgccgga gaatatgttg acctcggcgg cgtagccggt actgtgctga gtgtgcagat
    13261 tttctccacc accatgcgta ctgcagacgg taaaattatc gttattccga acggtaaaat
    13321 tattgccgga aatattatta acttctcccg cgagccagtt cgccgtaacg agtttattat
    13381 tggcgtggcg tatgattccg atatcgatca ggttaagcag atcctgacca atattatcca
    13441 gtctgaagat cgcattttga aagatcgcga aatgactgtg cgcctgaacg aacttggtgc
    13501 atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagt ggcgatctgc aaaacgtgta
    13561 ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc gccggtatca gcttcccgta
    13621 cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct
    13681 gaggccagat aatactctct ggcctctctc ttattagttt ttctgattgc caattaatat
    13741 tatcaatttc cgctaataac aatcccgcga tatagtctct gcatcagata cttaattcgg
    13801 aataccaaac gtgttttctt attactttca aggtcttgcg cttggcgcag ctatgatcct
    13861 accgctcggt ccacaaaatg cttttgtgat gaatcagggc attcgtcgtc agtaccacat
    13921 tatgattgcc ttactttgcg ctatcagcga tttggtcctg atttgcgccg ggatttttgg
    13981 tggcagcgcg ttattgatgc agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt
    14041 agccttcttg ctctggtatg gttttggcgc ttttaaaaca gcgatgagca gtaatattga
    14101 gttagccagt gccgaagtcc tgaagcaagg aagatggaaa attatcgcca ccatgttggc
    14161 agtgacctgg ctgaatccgc atgtttacct ggatactttt gttgtactgg gcagccttgg
    14221 cggtcagctt gacgtcgaac caaaacgttg gtttgcgctc gggacaatta gcgcctcttt
    14281 cctgtggttc tttggtctgg ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc
    14341 aaaatcacag cgcattatca atctggtagt gggatgtgtc atgtggttta tagccttgca
    14401 gctggcgaga gacggtattg ctcatgcaca agccttgttc agttaggcgc tatctgatgg
    14461 aaaaataaaa cagaggcgct aagcttgcct ccagaggtcc tgaattttag gcaagtcaaa
    14521 taacacggag agactaacgt gaagttcaaa gttatcgccc tggcggcatt aatgggtatt
    14581 agcgggatgg cagcgcaggc taacgaattg ccggatggac cgcatattgt cacctccggt
    14641 acggcaagcg tggatgcggt gccagacatt gccactcttg cgattgaagt caatgtggcc
    14701 gcaaaggatg ccgctactgc caagaaacag gcggatgagc gcgtcgcaca atacatttcc
    14761 ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct cagcgaactt acgcacccag
    14821 ccagattatg attatcagga tggtaaaagt atccttaaag gctaccgcgc tgtgagaacg
    14881 gtggaagtca cgctccgtca gttagacaaa ctgaattcct tgctggatgg cgcgctgaag
    14941 gcgggtctta acgaaattcg ttctgtgtcg ctgggcgtgg cgcagccgga tgcctataaa
    15001 gacaaagcac gtaaggcagc gattgataac gcgattcatc aggcgcagga actggcgaac
    15061 ggctttcatc gtaaactggg gccggtatat agcgtgcgct accatgtttc caactatcag
    15121 cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc cggtctcagc ccaggaaact
    15181 tacgagcagg ccgctattca gtttgatgat caggtcgatg tggtcttcca gttagaacct
    15241 gtggatcaac aacccgctaa aacacctgca gcacaataat acttgtagcc cgacgtattc
    15301 gtcgggctaa tgcctttacc cgatattgcc tcctttttct gtaattttct gtaaaaccat
    15361 gacgtaatca cctatctttg ctcgcggttt catcaaggat aggctgactg aaacggttaa
    15421 gcggacaagg atgcggggga ggggggatgg acatttttat ttcgaaaaag atgcgcaatt
    15481 tcattttatt agcgcaaacc aataatattg cccgggcggc agaaaaaatc catatgacgg
    15541 cttcgccatt tggtaaaagc attgccgcgc tggaagaaca aattggctat acgctattta
    15601 cccgcaaaga taataatatc agcctcaata aagccggaca ggaactgtat caaaaactgt
    15661 tcccggttta tcaacggctt tctgcgattg ataatgaaat ccataactca gggcgtcgtt
    15721 cacgagaaat tgtgattggc atagataata cttatccgac aattattttc gatcagttga
    15781 ttagtctcgg agataagtat gaaggcgtga ccgcccagcc agttgagttc agcgaaaacg
    15841 gcgttatcga taatcttttt gaccgccagt tagattttat tatctcccca cagcatgtat
    15901 ctgcacgagt tcaggaactg gaaaacctga ctatcagtga actaccaccg ctgcgattgg
    15961 gctttctcgt ttctcgccgt tatgaagagc gtcaagagca agagctgctt caggagctgc
    16021 cctggctaca aatgcgcttt caaaaccggg ctaacttcga agcaatgatt gatgcgaata
    16081 tgcgaccttg tgggatcaat ccaaccatta tttatcgtcc ctacagtttt atggccaaaa
    16141 tcagcgccgt ggagcgcgga cattttctga cggtgatccc gcattttgcc tggcgactgg
    16201 tgaatccggc aacgctgaaa tattttgatg cgccgcacag gccgatgtat atgcaggaat
    16261 atctttattc aatcagaaat catcgttata ccgccacgat gcttcagcat attgctgaag
    16321 atcgtgacgg gacaaaccat taacccagca tcgagccggt tgcaattaaa ttacggtgta
    16381 agtcgaagac gtggctaaga tcgtggtgaa tatgtccgcc aggcgcattt tccagatagc
    16441 gatgcagata atcccgatac ataggatgtg cacaattatc aatgatcgtg cgggcgcgtt
    16501 gaagcgggga aagaccgcgc agatcggcga tcccttgttc ggtgatgatc actttgacgc
    16561 tgtgttcgct gtgatcaaca tggctgcaca ttggcacgac ggttgagatc ttgccttctt
    16621 tagcaatcga tggggccata aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc
    16681 cgccgatgcc gttcatcaga tcgaccccgg ctacgtgcgt tgagttggca tgcccgtaaa
    16741 tatcaaactc caggccgacg ttcagagcga tgacgcccag acgacggatg atttccgggt
    16801 tattggaaat ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt
    16861 aaatcttgcg cagggaatcg gccgagattg tcaggctgga ggcgctggcc ccgctgattt
    16921 tgccggtttc cagtaaatgc accaccgatt cctgtagcac ttccgaatac atcataaacg
    16981 gaggaatttc cgggttttcc cccagacgcg ccattaccgc attattgata ttgcccacgc
    17041 cactttgcag cggcagaaat tccggcggaa tacgcccatg cgccatttcc tgcaataaga
    17101 acgtgaccac gttatcggca atctgctggc acatgggatt ttgcttatcc agcatattac
    17161 cggcgtcggg caagttggtt tccacgacgg cgacaatctt tttcggatcg atttgcacat
    17221 agcgggtacc gacgcgatcc attgcatgga agatcgacac gctattacgc cgcggtggcg
    17281 cgccaggaat cacaatatct gccagttctg caacgcgcgg atcgtgatag tgattaagtt
    17341 caatgatcac tttcttcgcc cgcagcagcc atgtcggcgc attaccgatc ccgctggtta
    17401 accagactcg accatccggt gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc
    17461 cgaagaaacc gtaattgacc atttgcgcca cttcgctcaa atgcaggtca acgaaactca
    17521 ccgcgccctg attgatcttt ttacgtaaac cggacgatgt ttgatatggc gcacgccagg
    17581 aaacagcatc agcgtcagaa agtacatcgt cagcggcggc gctgattgac gcacccgtca
    17641 gcaggcgaat ttgatacggc tttttggcct catgctgttc gttagctctg cgggcaatcg
    17701 cggtgggtag ggctttcggt gaacccgctg gggtaaagcc gctaaatgcc accatgtcgt
    17761 tatgctggat aatttctgcc gcttcatcgg cggtcatcct tgtccactga gtttccattt
    17821 ttactccttc gttcgcaggg attaatgacc aacgaaatta ggtttacgtt tttcgaggaa
    17881 agcgttcatc ccttcctggt aatcttcgct gtcataaacg gcgcggcgca tcccctgaat
    17941 acgttcaaat tcatcggagt tcatggtgtg tgcttcgccc agtacacgca gctcttcttt
    18001 gataacggca atggctaacg gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa
    18061 atcttccagt tcttccactt ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg
    18121 ggcggtgatt ggcgaagcgg taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg
    18181 ggtcaggttg tgaatgccga ccaggttata cgggacgccg aggtttacag gtgtcattga
    18241 gaaggttgag gtactggcgg cgatgatcag atcggaactc atgatcattt caaatgcgcc
    18301 accccaaaca ctaccttcca ccatcgaaat gatcggtttc gggaattttt ggatcatgcg
    18361 ggtgatttga cgcaacggat catcatagga gagcggatcg cgaccgccag acggcagttc
    18421 gtgaatatcg tgacctgcgg agaagacttt ggatccactc ggcgcgcgca aaatgataca
    18481 gcgaatttcc ggtcggttga gatcgcttaa tgcctgcata agatcatcaa taaagacttt
    18541 acttaaggca ttaagtttgc ggccatagtt aaactcaatg accgccactt tgttgatagt
    18601 gacaacgttc acatactgat aagacataaa aattccttta atcaaaatat tgcgtctgga
    18661 taaattcact gagctgccgc aggccggtgc gcggtgagag cgtattgttt ttgaccgcta
    18721 aaagcgtctg gcggtaatag cgatcgaaat cttcattcgc gaacaggtga ttcagtactt
    18781 cttcttcggt ttgtttacgc agccattcca ccgattgttg ttgccgcact tgctgtaaac
    18841 gaccactggc agttagcgcg gttttgaagt cgatgatggc gtgccagatc tcatcgattc
    18901 cacgtttttc cagtgcgcta caagtcagaa cccgtggctg ccattcgtcg tatttacgtc
    18961 gcagaatatg cagggcactc tcgtacatat gccgggcaat ggcgacattg gtatggttat
    19021 cgccatcgtc tttgttgata acgatcagat cagccacttc catcagccct tttttaatgc
    19081 cctgcagatc atcgccgcca ccggcaattt gcaacgagat aaaacagtcc accatgcggg
    19141 cgacttccgt ttccgactgc ccgacgccaa ccgtttcgac aatcactacg tcataacccg
    19201 ccgcttcgca taacagcatt aattcccgcg ctcgctgact ggcaccgccc agatgaccgg
    19261 aggatggtac cgggcgaata aacgccgtct cggcacgcgc caggtcattc atgcgggttt
    19321 tatccccgag aatgctaccg ccagtgaccg gactgctggg atcgaccgca ataaccgcaa
    19381 cttttaatcc atcacgaatc aacaacatgc caaaggcttc aagaaaggta cttttccccg
    19441 cgccgggtgt gccggtaacg cccagtcgca gggcgttacc gcagtacggc ataatggcat
    19501 caagcagttg cgtacttagc gcctgatgac gcgggtgacg gctttccacc agcgtcatgg
    19561 cctgggcgag tgtggcacgc tcaccctgac gtaagcggcg aatactttct gccagcgtgg
    19621 cttcattaat catgatgctg gcttatcaga ttcagtacgt cgcgcacact gtcgagcata
    19681 ggtgtacctg gaccataaat cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc
    19741 ggtggaatga cgccaccggc gactacgcag atatcttcgc gtccccattt tttcagcgct
    19801 tcgaccagtt ccgggatcag cgttttatga ccggcagcca gtgaggatgc gcccactacg
    19861 tgaacgtcat tttctactgc caggcgggcg atctcttcag gtgtagagaa catcgggctt
    19921 aaatctacgt cgaaaccgag atcggaatag gcgctggcga tcacttttgc gccgcgatcg
    19981 tgcccatcct ggcccatttt ggcgatcaga atgcgcgggc gacgaccatt gtcggcaagg
    20041 aactgctccg tttgcgcaac aatggcatcg aactcggagg ccgatttctc agattgatga
    20101 tagctttgcg caattacgcc ggtaacacac tggcttggca ccagataacg gtcgaaagcg
    20161 gcttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
    20221 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
    20281 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
    20341 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
    20401 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
    20461 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
    20521 tcgatctgtt ggataataac tctggcttgt ttgacgattt gatcggtcag cgactcaatg
    20581 taataggatc cggccagtgg atcgacggtg cggcagagtt ctgattcttc ctggatgatg
    20641 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
    20701 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
    20761 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
    20821 tgggtacgca gcgccagtga tttcgggtcc tgtgcgccaa atccactgac cgcttcgctc
    20881 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgatgccg
    20941 aagaagaacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
    21001 ttgatgtact caatcccatc agcgagcgta aatgctacct gctgcacgca gttggcaccc
    21061 gcttcaccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
    21121 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggcgggta aatataggtg
    21181 ttgcggcaga ggtactcttt gaggatatcg ttctgaatgg tgccggtcag tttatcaggt
    21241 gtaacacctt gctcttccgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
    21301 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
    21361 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cgcccgccac gcgcgggtta
    21421 tcggagtcgt agcctcggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
    21481 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
    21541 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
    21601 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
    21661 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
    21721 cggctcaatt ccttgttggc aagctgttgc cactcctgca cgttagacat tatttgctcc
    21781 gtaaaacgca atcaaaatga gggtatttgc gcctacggtg agctttttac gtgggtttgt
    21841 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
    21901 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
    21961 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
    22021 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
    22081 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
    22141 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgcacgaac gcttctgaag
    22201 aattaacgat atggcagggt acgctgccag gaggcagatc gaagttttgc tgcaaaaagg
    22261 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt aatgccgaac
    22321 gcgttacgcc gttagggaaa tatttttcgg caaacggttt tgagctgacg aacagatagt
    22381 cgagcgcacc aagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
    22441 cgccgaccac ttcgccgcga cgcagacgtt cctgagtgcg ggtttcatct tctacctgca
    22501 agttgaggcg gataggcgaa tcagccaaca caggagccag tgccggaagc aaccacgtcg
    22561 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    22621 cgcccagcca ctcttcttcc agcaactcca cctggcgcag cagtgccagc agtttttgcc
    22681 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    22741 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    22801 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    22861 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cataattttg
    22921 cgttgggtac agagcattta acgcatacaa aagcggtcgt aatggaagtt ttggctggca
    22981 atcccatttt catataaccc tttgctgagc gctttggcaa aactctgtgg gccgcaaaac
    23041 cagaaatggg tgtcaccttt atttgctatt ttatcagtaa gatagtgcga ctgtaggcgc
    23101 tcacctttat gcgcatcaag cagatgcagt gtgacactgg cctgggcaca aagtgaggta
    23161 agtttatcaa cataccatgc atcctcgctt cggctgacgc aataccagag atccacagga
    23221 acacgggacg cgccatgctg gttcagatac tcaagctggg cgataaaagg ggtaatgccg
    23281 atgcctcctg ctatccagac ctggcgggag caagggagag taaaatcaaa ctttccccat
    23341 ggcccttcaa cgagggcgga ctggccgatt tgtatcgagg atgccagagt cgaggtataa
    23401 tcccctaaat ttttgatggc aaagcgcaac gtgttcttct cttttacata tgaagcgata
    23461 gtaaaagggt gagattcttt actgtgtgcg aacgttaaga atgcaaactg ccccggacaa
    23521 tagtcgatgg gatgttccat ctccagtatg acttcggtaa tttgtcccgg atggttaaca
    23581 atgcttttaa cggtggcctg atgcttgctt ttattaccgt ttaatttcag cagcgaaatg
    23641 aaagccacaa agacgcctgg gatagtaatt aaaattaata aatacgcagc aggagtttcc
    23701 caccaatatg atttaaacag tgcagtaaat acatgaaaag caattgccag ataaaaaacg
    23761 gggaacaaac ggtgaacaag atgaaataaa tgatagggaa cttttttgct tagagacacg
    23821 aaaaccatcg ctatcataat aaacattgca cattcacccg ctaacaatcc cgcatgaagc
    23881 caattttctt gtactggcgt caggttaata tcaacgattt taccagggtg agtaagccag
    23941 ccaaaggcga cgaataactg gggtaatttt tcatccagcc agtgggcgaa agcaaaagcg
    24001 atggcgcata ttcctgccca cttatgtaat cgccatgctt tatccagacc gcccagcact
    24061 ttattaagcc atgatgaacg cagcgataaa cacatcgtca gcgtcatcag acaccacgca
    24121 agggttcccg taaaaaagag cgtgttttgt cgaatgaacc atattagcgc gattggcttt
    24181 tcacgaaaaa tatcgctgga cgacaaaaaa ataatacatg cgacaaagca aaccaacgta
    24241 cctgtaagat tagctttttt cattttttat ttcattttgg cagcggagca tagaacagtt
    24301 ccatgctccc gttaaataga gtgattattt atagaaaatc acagtggcgt gaatataatt
    24361 tttaccggag aaagaactta tataataatt gctggcttgt aactcttccg ctttctcttt
    24421 gatttggctt tcaacgcctg ctgggttgct attgtgtgta gacatgctca cttttgataa
    24481 ctgttcaagg ttattgtccg gtttatgtat ctcggttgcg gcaaatgcgt gtcctgtgag
    24541 agtgcaaaga aataacgcgg caaataaatt ttgcttaata gacataataa ctccttttat
    24601 attaaagata atgattaaat acttgtgtaa taagttcagg gatttgtgct aatgcatttt
    24661 gctcggcgag ttcgcgtccc ggttgggttt tgtcgacgcc ttccgcacgt aaaatatgtg
    24721 tattggtaat gcccattaat ttcagcgtgg cttgcaggta actttcctga tgatccagcg
    24781 ccagatgtgc ttcatcgcaa tagataccac cgcgcgtcga gacaataaac gtcggtttat
    24841 ccgtcagtaa cccaacggca ccagcggaag taaaacgaaa cgttaaacca gcctgacaga
    24901 cctgatctat ccaggttttg aggccagatg aaacgctgtg attaatcatc ggcgcgccaa
    24961 taatgagcac atcgcacgat gccagttctt caatagcggc gtgaatccca ttcacctcct
    25021 gccgaatttg tgcgctcccg cactctgctt tatcgcaaag cgccatgaag gtttcttcac
    25081 gtaaatgggg cggcggcgtc aggccaacgt cgcgatgaat aattgtggct ttcggaaagt
    25141 gttggcaaag cgcttctacc agcgtctggc ttaatttccg cgaacttgaa tgcttaagca
    25201 ttgggctact gtcgatatga agaattttca tgggcttgct ctcatttctg cgatgagaga
    25261 agtttaactg ttgtttatat ggtgattaaa tagacataat tgaacttcat tgttgtgaaa
    25321 atagaacaat catgatcgac tggaatgatt attactactt tgccctgatt gccgatgaag
    25381 gcagctacag ccaggcggcg ctgcgtgcgg gcgtgtcgaa atcggtatta agccgacgaa
    25441 tggctgcact ggaggagcgc cttggcgtac ggcttattca gcgaactacg cggcgtctgg
    25501 ccctgacggc ggcaggcgaa cagtttgctg tcgaatgtcg ggcgctgatg gcgcaggagg
    25561 aacgggccag agcggtggtg atggccgccc aggaaagccc acagggaatg ctgcgcgtca
    25621 gcgcgccggt gtttatggct gaaacatggt ttggtgagtt tgtcagtgag tttatcgccc
    25681 gttggcccct ggcgaaggtg caactgctgg caattaatcg cccggtggat atggtcgctg
    25741 aaggcattga tattgcgctg gttacggctt ccggcgcgct tgctgactcg acgctgcatt
    25801 ataaaaaatt acatacccag gctgatattt tagttgccag ttctcagtgg ctggcccgcc
    25861 atccgcaggt gaaagctatt gaagatctta accacgtcga cgggatcctt cggctggcga
    25921 acaatggcgg gagccagtgg caattaatta atggcgaaca ggagattacg ctgcagcctg
    25981 aggcgcgttt gcagtcaaat aaccttcgcg tcatgctgca agcagtactt ggtgggaatg
    26041 gcgtggcgtt attgcctcaa tttgcctgta aacaggggct tgcgaacggt gaactggtgc
    26101 gcctgtttgc accgtggagc ggcataccca gaccgttgta tgctttattt gcggggcgaa
    26161 aggggatgcc tgccattgcg cgatatttta tggatgagtt aaccacgcgg cttgccaacg
    26221 gggtctgaat cgcttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg
    26281 atcatgacgc aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag
    26341 cccggcacca ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcgctc
    26401 ggtacaatga aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa
    26461 ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac
    26521 gttgatggcg cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg
    26581 ctgacccgtg aaaaaatcat tgcttcggtt gcagaaaaat ttatctgtat tgcagacgct
    26641 tccaagcagg ttgatattct gggtaaattc ccgctgccag tagaagttat cccgatggca
    26701 cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc
    26761 gtggtgaccg ataatggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg
    26821 atagcgatgg aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct
    26881 aaccgtggcg cggacgttgc gctgattggc acacctgacg gtgtcaaaac cattgtgaaa
    26941 tgatctgacg ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg
    27001 tgtcacgttt ttacctggct attgtcgatt gctctaaata aatcctctaa accagcatat
    27061 tcatccaaga attacctttg cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt
    27121 gccgcaatat tattttttga tatgttgaaa ggcggacgca aatccgcaca caacatttca
    27181 aaagacagga ttgggtaaat ggcaaaggta tcgctggaga aagacaagat taagtttctg
    27241 ctggtagaag gagtgcacca aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac
    27301 atcgaatttc acaaaggcgc gctggatgat gaacaattaa aagaatccat ccgcgatgcc
    27361 cacttcatcg gcctgcgatc ccgtacccat ctaactgaag acgtgatcaa cgccgcagaa
    27421 aagctggtcg ctattggctg tttctgtata ggaacaaacc aggttgatct ggatgcggcg
    27481 gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa atacgcgctc tgttgcggag
    27541 ctggtgattg gcgaactgct gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg
    27601 caccgtggcg tgtggaacaa actggcggcg ggttcttttg aagcgcgcgg caaaaagctg
    27661 ggtatcatcg gctacggtca tattggtacg caattgggca ttctggctga atcgctggga
    27721 atgtatgttt acttttatga tattgaaaat aaactgccgc tgggcaacgc cactcaggta
    27781 cagcatcttt ctgacctgct gaatatgagc gatgtggtga gtctgcatgt accagagaat
    27841 ccgtccacca aaaatatgat gggcgcgaaa gaaatttcac taatgaagcc cggctcgctg
    27901 ctgattaatg cttcgcgcgg tactgtggtg gatattccgg cgctgtgtga tgcgctggcg
    27961 agcaaacatc tggcgggggc ggcaatcgac gtattcccga cggaaccggc gaccaatagc
    28021 gatccattta cctctccgct gtgtgaattc gacaacgtcc ttctgacgcc acacattggc
    28081 ggttcgactc aggaagcgca ggagaatatt ggcctggaag ttgcgggtaa attgatcaag
    28141 tattctgaca atggctcaac gctctctgcg gtgaacttcc cggaagtctc gctgccactg
    28201 cacggtgggc gtcgtctgat gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg
    28261 aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc aatatctaca aacttccgcc
    28321 cagatgggtt atgtggttat tgatattgaa gccgacgaag acgttgccga aaaagcgctg
    28381 caggcaatga aagctattcc gggtaccatt cgcgcccgtc tgctgtacta ataccccttc
    28441 tctgaaaatc aacgggcagg tcactgactt gcccgttttt ttatcccttc tccacaccgt
    28501 tgcgcattct actttgcgag tatgctccag aaaataaatc aaagtcagat caatgcgttg
    28561 tgtttattta atgggg
//
LOCUS       DAZGCQ010000061        28507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000061 DAZGCQ010000000
VERSION     DAZGCQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.061"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..1603)
                     /gene="yeeO"
                     /locus_tag="ACHK07_002931"
     CDS             complement(116..1603)
                     /gene="yeeO"
                     /locus_tag="ACHK07_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO8103235.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKEAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1764..1839
                     /locus_tag="ACHK07_002932"
     tRNA            1764..1839
                     /locus_tag="ACHK07_002932"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1797..1799,aa:Asn,seq:gtt)
     gene            complement(1877..2827)
                     /gene="cbl"
                     /locus_tag="ACHK07_002933"
     CDS             complement(1877..2827)
                     /gene="cbl"
                     /locus_tag="ACHK07_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO8103236.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2929..3846)
                     /gene="nac"
                     /locus_tag="ACHK07_002934"
     CDS             complement(2929..3846)
                     /gene="nac"
                     /locus_tag="ACHK07_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO8103237.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="ACHK07_002935"
     tRNA            4172..4247
                     /locus_tag="ACHK07_002935"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHK07_002936"
     CDS             complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHK07_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8103238.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHK07_002937"
     CDS             complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHK07_002937"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO8103239.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHK07_002938"
     CDS             complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHK07_002938"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO8103240.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLMALL"
     gene            complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHK07_002939"
     CDS             complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHK07_002939"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO8103241.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRKGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINTEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            7900..8034
                     /locus_tag="ACHK07_002940"
     CDS             7900..8034
                     /locus_tag="ACHK07_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO8103242.1"
                     /translation="MEEISLVKVWLREAGAHNVLVTSAVNNNGVAELLALLHTEEGCR
                     "
     gene            complement(8135..8464)
                     /gene="tmaR"
                     /locus_tag="ACHK07_002941"
     CDS             complement(8135..8464)
                     /gene="tmaR"
                     /locus_tag="ACHK07_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO8103243.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(8636..9694)
                     /gene="yeeA"
                     /locus_tag="ACHK07_002942"
     CDS             complement(8636..9694)
                     /gene="yeeA"
                     /locus_tag="ACHK07_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8103244.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRAVRK"
     gene            complement(9893..10366)
                     /gene="sbmC"
                     /locus_tag="ACHK07_002943"
     CDS             complement(9893..10366)
                     /gene="sbmC"
                     /locus_tag="ACHK07_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO8103245.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(10485..11651)
                     /gene="dacD"
                     /locus_tag="ACHK07_002944"
     CDS             complement(10485..11651)
                     /gene="dacD"
                     /locus_tag="ACHK07_002944"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO8103246.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            11860..13287
                     /gene="sbcB"
                     /locus_tag="ACHK07_002945"
     CDS             11860..13287
                     /gene="sbcB"
                     /locus_tag="ACHK07_002945"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO8103247.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNLIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGNNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(13330..13557)
                     /gene="tsuB"
                     /locus_tag="ACHK07_002946"
     CDS             complement(13330..13557)
                     /gene="tsuB"
                     /locus_tag="ACHK07_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO8103248.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(13571..14629)
                     /gene="tsuA"
                     /locus_tag="ACHK07_002947"
     CDS             complement(13571..14629)
                     /gene="tsuA"
                     /locus_tag="ACHK07_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO8103249.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASSLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(14809..16167)
                     /gene="plaP"
                     /locus_tag="ACHK07_002948"
     CDS             complement(14809..16167)
                     /gene="plaP"
                     /locus_tag="ACHK07_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO8103250.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(16157..16219)
                     /gene="yoeI"
                     /locus_tag="ACHK07_002949"
     CDS             complement(16157..16219)
                     /gene="yoeI"
                     /locus_tag="ACHK07_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO8103251.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(16434..17363)
                     /gene="yeeY"
                     /locus_tag="ACHK07_002950"
     CDS             complement(16434..17363)
                     /gene="yeeY"
                     /locus_tag="ACHK07_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8103252.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANCAIAGVYAKSDDGKSAI"
     gene            complement(17409..18233)
                     /gene="yeeZ"
                     /locus_tag="ACHK07_002951"
     CDS             complement(17409..18233)
                     /gene="yeeZ"
                     /locus_tag="ACHK07_002951"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8103253.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            18660..18710
                     /gene="hisL"
                     /locus_tag="ACHK07_002952"
     CDS             18660..18710
                     /gene="hisL"
                     /locus_tag="ACHK07_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO8103254.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    18686..18809
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            18856..19755
                     /gene="hisG"
                     /locus_tag="ACHK07_002953"
     CDS             18856..19755
                     /gene="hisG"
                     /locus_tag="ACHK07_002953"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO8103255.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            19761..21065
                     /gene="hisD"
                     /locus_tag="ACHK07_002954"
     CDS             19761..21065
                     /gene="hisD"
                     /locus_tag="ACHK07_002954"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO8103256.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            21062..22132
                     /gene="hisC"
                     /locus_tag="ACHK07_002955"
     CDS             21062..22132
                     /gene="hisC"
                     /locus_tag="ACHK07_002955"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO8103257.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            22132..23199
                     /gene="hisB"
                     /locus_tag="ACHK07_002956"
     CDS             22132..23199
                     /gene="hisB"
                     /locus_tag="ACHK07_002956"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO8103258.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            23199..23789
                     /gene="hisH"
                     /locus_tag="ACHK07_002957"
     CDS             23199..23789
                     /gene="hisH"
                     /locus_tag="ACHK07_002957"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO8103259.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            23789..24526
                     /gene="hisA"
                     /locus_tag="ACHK07_002958"
     CDS             23789..24526
                     /gene="hisA"
                     /locus_tag="ACHK07_002958"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO8103260.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSAEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            24508..25284
                     /gene="hisF"
                     /locus_tag="ACHK07_002959"
     CDS             24508..25284
                     /gene="hisF"
                     /locus_tag="ACHK07_002959"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO8103261.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDEGRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            25278..25889
                     /gene="hisIE"
                     /locus_tag="ACHK07_002960"
     CDS             25278..25889
                     /gene="hisIE"
                     /locus_tag="ACHK07_002960"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO8103262.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(25986..26963)
                     /gene="wzzB"
                     /locus_tag="ACHK07_002961"
     CDS             complement(25986..26963)
                     /gene="wzzB"
                     /locus_tag="ACHK07_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO8103263.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(27110..28276)
                     /gene="ugd"
                     /locus_tag="ACHK07_002962"
     CDS             complement(27110..28276)
                     /gene="ugd"
                     /locus_tag="ACHK07_002962"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO8103264.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         7514 a         7480 c         6777 g         6736 t
ORIGIN      
        1 ctgaactaca gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc
       61 aaagcctgtt ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt
      121 ccgacacagc tggctttttt tcacactttt gcggctcgga tcgagggtat ttccatagcc
      181 aacgtccagt aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca
      241 ttcccatcca gacaccaacc acaccccagc caagcatgat tcccagcaca taaccgacga
      301 caacccgaca accccacatg ctcaacatcg aaacccacat ggcgtaacgg gcatcacgag
      361 cacctttaaa tccagcgggt agcacccatg aggcggacca aataggcata aataaagcat
      421 ttagccaaat cagaatcaca acgacatgtt taacctgtgg atcctgggtg taaaacgatg
      481 ccataacccc ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggaca
      541 gccagaacac atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc
      601 ggcctgtaat gatcgtagaa gcagagccga gcgcacttcc gggtaagttg ataagagccg
      661 caattgaaaa cgcgataaaa tttccggcaa taacactggt ccccatcccg gcaacgaaca
      721 tttgggttaa taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa
      781 tccccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa
      841 tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat
      901 aacgagaaat ggttaaaccc aacccagccc cgacaaatcc cagtcccggc caggagaaaa
      961 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     1021 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaatt agagtgatgg
     1081 cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt
     1141 ctgtcgtggc atcacccgcg acgaaatcaa taatttgctc gccaaaatga tgaataagcg
     1201 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     1261 ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga
     1321 tagcagcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca
     1381 tcgcttcttt tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac
     1441 aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
     1501 tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     1561 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag
     1621 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     1681 atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga tttttcgccg
     1741 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     1801 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     1861 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     1921 gccgcttgat atcctcaact gaaagtcctg cattacaaag ttccagaaag cgccagacat
     1981 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcaaaaagat
     2041 gccgcgtatc caggcggatt aaattctttt cctcttgttc accgctggat tgttcggcaa
     2101 ctattccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac
     2161 ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc
     2221 ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgactcc agcgtcaatg
     2281 gtgtaatttg cgtcaaggga tgatcgagtg gaacaagcaa actatggtgc caacgaaacc
     2341 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     2401 cttcaccatt atgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc
     2461 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     2521 gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa
     2581 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     2641 ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     2701 catcctccag ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg
     2761 caacctctgt caggttgtaa tcctgacgtg cagcttcgcg gattatcttt agttgttgga
     2821 aattcacggt aaactccggg cagttcagat attccgttat tgttaaagtc taacgcccgg
     2881 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     2941 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actctttcac
     3001 tgcctgtgcc tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact
     3061 catggaaggc gtggtaatac gtgacatcca cccatttact gcgccacata acgaacgcgc
     3121 ggccgattcg ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtggc
     3181 aatagactca atttcaccaa tgacttttgc cgtgagtcgc cgtagggaaa acgcctcatc
     3241 aacacgaagt ctaacagcgc tgtaatcact ggggagaaag aggttcattt gtgcaatagc
     3301 attaacatca acgctttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt
     3361 cagcaaagcc tgactggata ccccagccac aggggaatgc tcataaatca ccgccatatc
     3421 gagttggtga tttatcagtt tttcgttaag cactgcaccg ctattttcat gaagatagat
     3481 aacgatctcc gggaattcag cgcgaaccgc ttgtaataag ggcatggtga tagatgacgc
     3541 agcggttcct ggcgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg
     3601 caccgccagt tgggcctgct cacactgacg taaaatggcc cgcgcatggg tatagagaat
     3661 ttttccggcg tctgttggcg taacgccccg ttttgtgcga atcaaaagtt gttgatttaa
     3721 ctcaccttcc agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc
     3781 agcagcctga gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa
     3841 gttcatgttg cctccggttt ttgagaatcg gcccaagtgc cgccattact tacaaccaga
     3901 ctgcaagatg cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat
     3961 aagagattat gatagattcg tcattggctc ttttcaccag cagtcgcgtt cccctgcccc
     4021 ataaaaggag agtatgcacc acgatggttc ataaccaata agattgaaag ctcaccactt
     4081 tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgctttcta gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga
     4321 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     4381 cacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     4441 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     4501 gttcgagcca tctggttcag tggtgtattt tactggctgg tcgataattt gcacacgcgt
     4561 cccaacagga acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc
     4621 ctgacttacc cggagcccaa taccaaaatt ggcattggta ccgtggatgg catacaacct
     4681 gccaatataa atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg
     4741 caaactctcc cctcgtttcg catattcgcg ccgagtgttc ggcgttggcg tccaggttgg
     4801 agcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     4861 gccgatacca ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg
     4921 catctcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     4981 cggaatggtg agttgcgagc cagacttcgg caagaaaaca tcagcgcccg ggttcgcttc
     5041 cagcatgtta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt
     5101 gtgatcagga acagttacag taaacgactg ccccactaaa cggctaccct ctggaggtaa
     5161 tggataagtt accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat
     5221 atttacacga cgcatcatgt ccctttccta tgtcgcgaaa gctatccgtt aagtatagct
     5281 tttatcagac ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa
     5341 tattactggc agcaagttcg cccatgttgt tgtatatcgc acaggcagct tcgatgatgg
     5401 gcatcgccag agctgcgcca ctcccctcac ctaaacgcat ctccatattg agataaggct
     5461 ccagccccaa atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa
     5521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa
     5581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca
     5641 ttccgaccaa atcaaatcca cccacttttg ccagaacatc aacgccatcc tgcggatttg
     5701 gttgattcaa cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg
     5761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     5821 ttgctgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     5881 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgac
     5941 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     6001 taagcccggg gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcac
     6061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa
     6121 tggctgtcac ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     6181 acataaccag taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcctg
     6241 ccagttgtat ggcaagcgcc tccagccttc caaggctacc aacaggtttg agtaacccgt
     6301 caatatgccg ttgtgcacgc gacatagcgg caggatcgat agcagggatc gtattcagta
     6361 aatcggcaag tgtttgcatg tcacgtccct ttatagcagt gccatcagga agaccagttc
     6421 accaagctct atggctgctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag
     6481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     6541 acccggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     6601 cccatcaatt ttgccaatga acacattgcc aagtccttct tcccgggcat aacgatgacg
     6661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     6721 gatcggttcg ccacgcagag ccaactcgct taataccaga atctttgcca gtacaacaaa
     6781 aatcaatgcc agaccgccgt gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     6841 atcgcggcta cgtgcagaaa atacgccatc acaggtatcg gcgaggccgt ccaggtggaa
     6901 tcccccggtc atcagcgcca gcacaagcac gctaaacagt gccgccagtg gtacgccaca
     6961 ccatgcctgc agcaccatga aaaccagccc gctaatcgcg ccaagcaata atccaattaa
     7021 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     7081 cggtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt
     7141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc cagctgctga
     7201 tttacccgcc cggcaatatc acgaaaatga cgtgccagac gactctccgg cacaatcccc
     7261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     7321 aacgactgaa tctcagtatt aatcacctgt tccatcgcct gataatccca ttcatcaggg
     7381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattccagc
     7441 aacacagctt catgcgggtt aatgtctgca tgaattaatt catcaagatg ttgccaacgc
     7501 tccaccgtgc gccagtgcgc cgggcggcct ttccgatgat gctctatccg tgcagccatc
     7561 tcatcatcaa ggatttggga ggtggcgata tacagaacct gtgaagagtc cccaataagc
     7621 gcctctgcgt ggcgactttt cccgctccgt gcgccccccg tcaccaaaat catcataccg
     7681 actcctgatt caattgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg
     7741 ctgaccgcaa aacatttaca cgattaatac tgcgcgtaat ataattaaaa atccagcatt
     7801 ctcaatccat tacgaaagaa gatggcattg tggaatagcc agacttcctc gtgttaacgt
     7861 ccgatcattg gagtagtcac caaagcagat ttagccagca tggaagaaat atctttggtg
     7921 aaagtttggt tacgggaagc cggagcgcac aacgtgttag taaccagtgc ggttaataac
     7981 aatggagttg cagaactttt agccttgctg catacagaag aaggctgtcg ctaatttcga
     8041 caggttctca gatgagaatg atgactttga tacacagaaa agaatcaatg agcaatgagg
     8101 gttgccgggc aaccctcatt gaataaaaca ggaattactt cgcttcgccg tttttcattt
     8161 cgcccatagc tttcagcttt ttggagatat cgcggcgttc tttggagagc tcggcatttt
     8221 tgatgatgta atcgtcaaca cgatcttcat agtcaccttt catgctggcg atgatgccct
     8281 ggattgcttc aacgctcatc cccggcttga tgtaatcgct caggttgtcc agcagcagga
     8341 cgcgcttctg gttgtcacgg atctttttct caacgtcctg aatttcacgt tgcagtttgt
     8401 tcttacgacg gaacagacga acaaattcca gtacgtcctg gaatgaaggc ttggtagttt
     8461 ccatttttat acccctgata atgtgagagt cggattcgtt taagcaaccg ctattcgtta
     8521 gggccaacat tactcatagc tgccgcagat gacaatgctt ttatcccttt tcactatcat
     8581 accctttatc cttgctgaat cgaagcagca gcaagatgat tctgaagttc aggaattatt
     8641 tgcgcacggc ccggcaaatc agattcgata gcaactcaag ctgatgtgcc gtttccatgt
     8701 tcagccacac ataaccatag attggtgttt ccacaacctt caggtcatgg tggttattga
     8761 gcaactgacg cagctcttcc acagcatcgt tcaatttttc cgtattggca aaaaccggct
     8821 gcggattacc ttcgtacagc gcatgaacaa ggctcagcag tatttgctgc atcatgtgct
     8881 gggtatcacg aagtttttgc gcgttcaata acacgaaatg gctggggcgc gtggcccagt
     8941 acgcattgat ttgcaactcc agcatacaaa ccagattgcg attaattgtc tggatacctt
     9001 catatatcga ttttggaata cgggtttctt tgctggcggg cgcaatcaac ccacgcattt
     9061 tcacggcatc ggtcaggagt ttttgtagat ggctttccag acgtgggcgt tcgagtaagt
     9121 tcggtgagaa tgcagattga tagacccgat tatactcggt cagacttttc gccagttgaa
     9181 tgcgccagtg gatgaacgcc cgttgtggcc agataccggt aaacaacatt gccagtaaag
     9241 agccgaggat cacatcgccg cttcgccata acgccgtatc aatttcacct gtcggggaac
     9301 caacaacaat tgccagcgtc accccaatca ataaaccttg atacggtttc ttgcccagcg
     9361 ccagccaacc gcaaaggaac atggccgccg cgcaccagac taacatcagc ggtaacgaga
     9421 ttaactccag ttgcagagcg ataagaccta aaatcgaacc caacaccgta ccgccaatac
     9481 gttcgaaggc gcgagggaca acgttacccc agaacgagat tggccccata atcaccacca
     9541 tggtgaccag cggccaggtg ctttccggga tagtaaacag gcggataatg agaaaagtga
     9601 gcaggaatgc cagcgcgacc cgagtaccat gcacaatgcg gtaatggcgg tatacccgga
     9661 tttcaaacgg gcttaatgac ttatcggcac gcacgcagaa actccgtaat gaagattaaa
     9721 agcgctcata actcatctgt cggccagaag gcgcgcctct gaattatccg acggacaaac
     9781 cgggaacagt gtaatttccc ggcaggagcg aaaaaattga aaggcgcatc ttactctttt
     9841 cgctttcaaa aaaaagagtg gtcatcgcgt taacacaccg ccctgagatg aattagtgat
     9901 gttttggctg caccgcaaca tacatttcga tatcccagta cccatcttcc gcgccattgt
     9961 tcaaataaac ctcgaagcag ggctttggta acatttcata agcactgtcc tgcaagaggc
    10021 tattaaagaa ctgataccag ggtttagcaa aatcatcacc gactacacga gctaccgcca
    10081 ccgcatactg accacctgaa atttctgtca gaatgacgcc ctcactgttt tcggggagcg
    10141 taaagtaacc cggcaccgtc acgacggtgt cgcagcgtaa tttttcggcg ggtgtttcat
    10201 ctggattgtc gtaatagaca gcaacccatt ccttcggcac aatatttttg ctatctaccc
    10261 acatcatcaa ctgctcaaaa cctttcttta ccgtctgttc ccacgggcca acgagatgga
    10321 aacctgcaac ggtacgttta tcttcctgct taatctcgta gttcatgacg cctccattaa
    10381 cactgttttt atatacagta tagttgcaat attaaaacca caaggaatga gtgttgatta
    10441 tgcgagcaga ctcgcactcc tgccagtctg ctgcaaaaga aaggtcaggc cttatggtgg
    10501 aaataatcac tcaggcggga aaacatgccg ccttccccga cagattccag ggtaaccagc
    10561 ggccagtgcg ccacctgttt atcacggtcg taaagttcaa tttcccctac ccgctgatgg
    10621 gcgctaattg gcgcggtgag ctctttacca tcaagggtat atttggcttt gatatgtgga
    10681 atttcggctt tcggtagcac catccagaac tcttgttccg ttcccaggtc gatattttct
    10741 ttatcgccat accagatgcg ttccgttccg acctttttcc cacggtgcaa aatttgcacc
    10801 gtagtaaagt tttgttgccc ccaacgtagt aattttcttg cctcttcctc acgacctttt
    10861 gcactgtcag cccccattac cacggcaatg agacgacgct gcccatctac agccgaagca
    10921 atgagattaa acccggcacc agaagtatga cccgttttca ggccgtcaac attcatggtt
    10981 ttatcccaca acaacccgtt acggttttgc tgggtgatac cgttccaggt gagacttttc
    11041 tcactgtaca tatgataaaa ctcgggctcg ccgtggatga tagcgcgaga aagtacagct
    11101 aaatcataag ccgagctatg ctgacctggc gcatccaggc catgcactgt ttcaaaatgc
    11161 gtatccttga gatgcagctt ctcggcatag ttgttcatca tttcaacaaa ctgccgttgc
    11221 ccaccggcaa tatagtcagc cagtgcaaca caagcgtcat ttccggaatc cacaattaaa
    11281 ccacggctta aatcacgtac cgatacgcga tcgccctctt tcaaaaacat cagtgaagaa
    11341 ccgacaaaca ccggattatc tttcgcccac gcatcgcgcc ccacggtgac aatatcgtct
    11401 ggcgtaatgc gatgactatc gatagcgcga tccacgacat aacccgtcat cagctttgtc
    11461 aggctggcgg gattgcgctg ttgatgctca ttacccgcgg tgaggatctg tccggtggtg
    11521 taatccatca gtacccagga cccggcatga atctctggag gctgaggtga aaaaggaatg
    11581 ttttccgccg caaaaccaga cgataagtta aaaacgaaca aagaagcagc aataataaga
    11641 cggcgtttca acagcaaacc ctcaggagtt tcaaatagct gttcttttta cggaaatact
    11701 tatgaactgg ctggaataaa gtgcaaaaaa atgtgactac cctctcattt ttatctgaca
    11761 tgatctgttg ccactcgctg ccaaattgtg gcgctaaagc tgattagcac ggtgatattt
    11821 gatactctgg cagacagcag aaataacgga tttaacctaa tgatgaatga cggtaagcaa
    11881 caatctacct ttttgtttca cgattacgaa acctttggca cgcaccccgc gttagatcgc
    11941 cctgcacagt tcgcagccat tcgcaccgat agcgaattca atctcatcgg cgaacccgaa
    12001 gtcttttact gcaagcctgc ggatgactat ttaccccagc caggagcagt attaattacc
    12061 ggtattaccc cgcaggaagc acgggcgaaa ggagaaaacg aagccgcgtt tgccgcccgt
    12121 attcactcgc tttttaccgt accgaagacc tgtattctgg gctacaacaa tgtgcgtttc
    12181 gacgacgaag tcacacgcaa cattttttat cgtaatttct acgatcctta cgcctggagc
    12241 tggcagcatg ataactcgcg ctgggattta ctggatgtta tgcgtgcctg ttatgccctg
    12301 cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt tcgccttgag
    12361 catttaacca aagctaatgg tattgaacat agcaacgccc acgatgcgat ggctgatgtg
    12421 tacgccacta ttgcgatggc aaagctggta aaaacgcgtc agccacgcct gtttgattat
    12481 ctctttaccc atcgcaataa acacaaactg atggcgttga ttgatgttcc gcagatgaaa
    12541 cccctggttc acgtttccgg aatgtttggg gcatggcgcg gcaataccag ctgggtggca
    12601 ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta tggtggattt ggcaggagac
    12661 atttcgccat tactggaact ggatagcgac acattgcgcg agcgtttata taccgcaaaa
    12721 accgatcttg gcaataacgc cgccgttccg gttaagctgg tgcatatcaa taaatgtccg
    12781 gtgctggccc aggcgaatac gctacgcccg gaagatgccg accgactggg aattaatcgt
    12841 cagcattgcc tcgataacct aaaaattctg cgtgaaaatc cgcaagtgcg cgaaaaagtg
    12901 gtggcgatat tcgcggaagc cgaaccgttt acgccttcag ataacgtgga tgcacagctt
    12961 tataacggct ttttcagtga cgccgatcgc gcagcaatga aaattgtgct ggaaaccgag
    13021 ccgcgtaatt taccggcact ggatatcact tttgttgata aacggattga aaagctgttg
    13081 ttcaattatc gggcacgcaa cttcccaggg acgctggatt atgccgagca gcaacgttgg
    13141 ctggagcacc gccgccaggt attcacaccc gagtttttac aaggctatgc agaagagatt
    13201 cagatgctgg cgcagcagta tgccgacgat aaagagaaag tggcgctgtt aaaagcgctt
    13261 tggcagtacg cggaagagat cgtctagttt gaagcctccg ccgctgttac ggcggaggac
    13321 aatgttgggt caggcttttt gaacggtgat gctccacgca gcatcgccaa tctgctgata
    13381 atcggtgatg gcatgtcctt cttctgccgc ccactgtggg attgcttcag ttgcctgggt
    13441 gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg ctgcttttgc
    13501 ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa tcgccatctt
    13561 tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc ggtcgaacat
    13621 agacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca acccagccct
    13681 gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca cctgcaatac
    13741 tggcaccgaa gcccatcagt acaccaccta acccgctacg taatgttgtt tgtgcatcag
    13801 ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgacccc acgaagatcc
    13861 ctaacaccaa gaaaacgccc cagttaatgt atttcacgtc acctgcgacc agaaattgca
    13921 gaatattggc cgttggggaa gtgattccta acccaaacat gcgtccggtt gcttcactca
    13981 agggccaggc taaaagcgca atcaaaccga tgagtacagc agtgacaaag ggatgccagc
    14041 gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc gcgactttca
    14101 gttttggctt cttcaactct ttcatcacca cccagagcgt tatcaccagc aaaacggcaa
    14161 ccaacggcca cacagacaaa ttaaaagtct cagcaataga gttatgttca gtactgtagt
    14221 gctgcaaggt ttgatttaaa ctactggcat gtggagaacg catcaccgca ctcatcacca
    14281 tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga taccaggtcc
    14341 cggtggcaca tccacccgcc agaacaattc ccagcccgaa gatataccca cctataacag
    14401 taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga attaacgcaa
    14461 aaacccccac gctttgtacc gaaattgcaa tcagcagggc gtaaaacatg cgattatttt
    14521 tcgcgatata catatcgcga aaaccacctg tcaggcaaaa acgcccacgt tgcatgacaa
    14581 atcccagcag agcaccacaa attagcccgc ttaatatcat tgaaaacata aattaattaa
    14641 ccagatgaat gttaatgagg aaattattca tgactggtgg aatgcagacc aataaccaaa
    14701 ttctctaaat tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaaa
    14761 ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc
    14821 ttcgtactgc ggaaccgggt tacggaagct cttggtgacg caagccaggt aaatcagacc
    14881 gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc
    14941 accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt
    15001 cttattacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa cgaaggtgaa
    15061 cgccaccaac gcaccaaagt tgatcagcgc cgtagccatc accaggtcga agttgattgc
    15121 cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg
    15181 tacataaccg aagaagcttt tcgggaatac gccgtcacga cccattacgt acatcagacg
    15241 cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag
    15301 cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc
    15361 cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat
    15421 gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc
    15481 atctttggtt tcttccgaca ggttgctgat gccgtcaaag ccggtaaagg agaagcacag
    15541 gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga atggacgagt
    15601 gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat
    15661 caccgcgatc agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt
    15721 cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga
    15781 tgggatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg
    15841 cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat
    15901 ggatttctgg gcgtaagtat atgcagagcc agcagaagga tagcggcgaa ccagcttccc
    15961 gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg
    16021 accatccgtg aggcctgaaa cgataccaaa agtatcaaac agcgtcatcg gctgcatata
    16081 ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga
    16141 ggtgtttgga gtaacgttat gcgacatggt cattctcctt tacggtgata accgtcgcgt
    16201 aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc gatttttagt
    16261 aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa
    16321 ataaccgacg ctgatgaaac gtcggttttt agtcattttt tgacagcggc gcattgtgcc
    16381 taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt tctttaaatg
    16441 gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcacaatt tgctaaaatt
    16501 tcatcccgcc accagccact ggcaagtcct gcggtctgtt cattccagcc aatccagacg
    16561 ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact atcgagaaaa
    16621 cgttgcgcca gataacgggg taaatagccg cagccgaggc cgctaatttg cagctccagc
    16681 ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg tgaggccgta
    16741 gaagcgcctg cctgcgcagt gtcccccacc acaatcgccc gatatcgttt aatgatacga
    16801 cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc aaagacttgt
    16861 tccagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc atgcatcgcg
    16921 ccaacaataa tatccgctcg cccctgagtc agggcatccc aggaaccacc gagtacgcca
    16981 ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat cagtggcgca
    17041 agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc ccagccttca
    17101 tgcagtttga tcgcctgctt ttccagttcc cgcacggtat gcagaacttc ccgccctttc
    17161 tctaataaca tttttccggt gcgggtgaat ttagcgcggt gaccgctacg atcaagcaat
    17221 tgaatattaa ggtcgctttc cagcttgtga acggtgtaac tcagagcgga cggcgtcttg
    17281 tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc
    17341 aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg tcctgctggc
    17401 gtgatcgttt actccagcgg cattaccagc ggatcgggat actggtattc aaatcccagt
    17461 tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag actatttctg
    17521 aactgcggag gttccagccc cagtaaacgg gccatctgcg gatagaaaac attacgcgca
    17581 gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg taacaacaga
    17641 gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc accatcaggc
    17701 gcggtttttc cggcaaagaa gcgtccggga tgacgccccg gtccgaccag gcccgcaaga
    17761 cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat
    17821 actcgtccac tgttggttac cggattacgc ggtgtagttt ctttcaccgt gccttgcgca
    17881 tcgccataga cagatgttga gctggtaaaa ataatgcggg gaatacgatg agccagcgcg
    17941 ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc gctacgacgt
    18001 gccggaagcg taatgaccag cgcatcggca tccatcaggg catccagatc gtcagaatcg
    18061 caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat tcgggccgct
    18121 tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac
    18181 atcgccagcg gcatgcctaa ccaccctaac ccgacaattg cgaccttttt catccgttat
    18241 ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga caattcattc
    18301 tatgaatgaa atcgaaacgg ttagcaaata taccattgcc tgatgcgctc cgcttatcag
    18361 tcctacaaaa acatcaatat attgaattta ggcaacattt gtagaccaga tatggcattc
    18421 acgccgcatc cggcaggaac aaagcccact ttagttacag aacacaatgt tcttgagggt
    18481 tttaatgaca tattttcacg attacttttt taacctagtc actcttagcc ccctcctttc
    18541 cccgctcatt cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct
    18601 aacgtgaaag tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta
    18661 tgacacgcgt tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc
    18721 gatgtgtgct ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc
    18781 ggaagatcac cttccggggg cttttttatt gcgcggttga taacggttca gacaggttta
    18841 aagaggaata agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc
    18901 cgtttaagtg atgactcacg cgaattgctg gcgcgctgtg gcattaaaat caatcttcac
    18961 acccagcgcc tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac
    19021 gacgacattc ccggtctggt aatggacggc gtggtagatc ttgggattat cggtgaaaac
    19081 gtgctggaag aagagctgct taaccgtcgc gcccagggtg aagatccacg ctactttacc
    19141 ctgcgtcgtc tggatttcgg cggctgccgt ctttcgctgg caacgccggt tgatgaagcc
    19201 tgggacggcc cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc
    19261 aagcgttatc tcgaccagaa aggcatctct tttaaatcct gcttactgaa cggttctgtt
    19321 gaagtcgccc cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc
    19381 acgctggaag ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcttgcctg
    19441 atccagcgcg atggcgaaat ggaagaatcc aaacagcaac tgatcgacaa actgctgacc
    19501 cgtattcagg gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc
    19561 gaacgtctgg atgaagtcat cgccctgctg ccaggtgccg aacgcccaac tattctaccg
    19621 ctagcgggtg atcaacagcg cgtagcgatg cacatggtca gtagcgaaac cctgttctgg
    19681 gaaacgatgg aaaaactgaa agcgctgggt gccagttcga ttctggtcct gccgattgag
    19741 aagatgatgg agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg
    19801 cggagcaaca acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc
    19861 gcactgttaa cgatattctc gataacgtga aagcacgcgg tgatgacgcc ctgcgggaat
    19921 acagcgcgaa gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gaggaaattg
    19981 ccgccgccag cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga
    20041 atattgaaac cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag
    20101 gcgtgcgttg ccagcaggtc acgcgcccgg tcgcttcagt tgggttgtat attcctggcg
    20161 gctccgcccc gctcttctca acggtattaa tgctggcgac tccggcgcgt attgcgggct
    20221 gtaaaaaagt ggtgctgtgc tcaccgccgc cgattgccga tgagatcctt tatgcggcgc
    20281 agttgtgcgg tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg
    20341 cgtttggtac ggaatctgtg ccgaaagtgg acaaaatctt cgggccagga aacgcctttg
    20401 tcaccgaggc aaaacgtcag gtgagccagc gtctggacgg tgcggcgatc gatatgcccg
    20461 caggcccgtc ggaagtgttg gtcattgccg acagcggcgc aacgccggat ttcgtggctt
    20521 ctgatttgct ttctcaggct gaacacggcc cggactcaca ggtgatttta ctgacgcccg
    20581 ctgctgatat ggcgcgtcgc gttgccgaag ctgtcgaacg ccagttggca gaactgccgc
    20641 gtgccgaaac cgcccgtcag gcactgagcg ccagccgcct gatcgtgact aaagatttag
    20701 cgcagtgcgt agagatctcc aaccagtacg gcccggagca cctgatcatc cagacccgca
    20761 acgcccgcga tctggtcgat ggcatcacca gcgccggttc agtatttctt ggtgactggt
    20821 caccggaatc ggcaggtgat tacgcctccg gcaccaacca cgttctgccg acttacggtt
    20881 acaccgccac ctgttccagc ctcgggctgg cggatttcca gaagcggatg accgtgcagg
    20941 aactgtcgaa agaaggcttt tctacgctgg cttcaaccat tgaaacactg gccgccgccg
    21001 agcgcctgac cgcccataaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag
    21061 catgagcacc gtgactatta ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta
    21121 tcagtcggcg cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaataccc
    21181 cacagccgtg gagtttcagc ttactcagca aacgctcaac cgctacccgg aatgccagcc
    21241 gaaagcggtg attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt
    21301 cagccgtggc gcggacgaag gtattgaact actgattcgc gctttttgcg aaccgggtaa
    21361 agacgccatc ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat
    21421 tggcgtcgag tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat
    21481 ttccgacaag ctggacggcg taaaagtggt ctatgtttgc agccccaaca acccaaccgg
    21541 gcaactgatc aatccgcagg attttcgcac cctgctggag ttaacgcgcg gtaaggcgat
    21601 tgtggttgcc gatgaagcct atatcgagtt ttgcccacag gcatcgctgg ctggctggct
    21661 ggcggaatat ccgcacctgg ctattttgcg cacactgtcg aaagcttttg ctctggcggg
    21721 gcttcgttgc ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat
    21781 cgccccctac ccgctctcga cgccggttgc cgacattgca gcccaggcgt taagcccgca
    21841 ggggatcgtc gccatgcgcg aacgagtggc gcaaattatt gctgaacgcg aatacctgat
    21901 ggccgcactg aaagagatcc cctgcgtgga gcaggttttc gactccgaaa ccaactacat
    21961 tctggcgcgc tttaaagcct ccagcgcagt gtttaaatct ttgtgggatc agggcattat
    22021 cttacgtgat cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac
    22081 ccgtgaagaa agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag
    22141 aagtatcttt ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag
    22201 gtggaccgtt ttgacaaact cgcctttgaa ccgggcgtga tcccggaact gctgaagctg
    22261 caaaaagcgg gctacaaact ggtgatgatc actaatcagg atggtctggg aacacaaagt
    22321 ttcccgcagg cggattttga tggaccgcac aacctgatga tgcagatctt cacctcgcaa
    22381 ggcgtgcagt ttgatgaagt gctgatttgt ccgcacctgc ccgcagatga gtgcgactgc
    22441 cgtaaaccga aagtaaaact ggtggagcgt tatctcgctg agcaagcgat ggatcgtgcc
    22501 aatagttatg tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt
    22561 aatggtttac gctacgaccg cgaaaccttg aactggccga tgattggtga gcaactcact
    22621 aaacgagacc gttatgccca tgtagtgcgc aacaccaaag agacgcaaat tgacgtccag
    22681 gtgtggctgg atcgtgaagg tggcagcaag attaataccg gcgttggctt ctttgatcac
    22741 atgctggatc agatcgccac ccacggcggt ttccgcatgg aaatcaacgt caaaggcgac
    22801 ctctatatcg acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta
    22861 aaaatggctc ttggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac
    22921 gaatgccttg cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atataaagct
    22981 gagtttacct accagcgggt tggcgatctc agcactgaaa tgatcgagca cttcttccgt
    23041 tcgctctctt acaccatggg cgtgacgctc cacctgaaaa ccaaaggtaa aaacgatcat
    23101 caccgtgtag agagcctgtt caaagccttt ggtcgcaccc tgcgccaggc catccgcgtg
    23161 gaaggcgaca ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata
    23221 ccggctgcgc caatttgaac tcggtgaagt cagccattgc gcgccacggt tatgaaccca
    23281 aagtcagccg tgacccggac gtcgtgttgc tggccgataa actattttta cccggcgttg
    23341 gcactgcaca agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag
    23401 cctgtaccca accggtgctg ggcatctgct tagggatgca actgctgggg cggcgcagcg
    23461 aagagagcaa cggcgtcgac ttgctgggca tcattgacga agacgtgccg aaaatgaccg
    23521 actttggtct gccgctgccg catatgggct ggaaccgcgt ttacccgcag gcaggcaacc
    23581 gcctgtttca ggggattgaa gatggcgcgt acttttactt tgttcacagc tacgcaatgc
    23641 cagtcaatcc gtggaccatc gcccagtgta attacggcga accattcact gcggcggtac
    23701 aaaaagataa cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta
    23761 agttgctgaa aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg
    23821 caccgtggtg cgtctccatc agggcgatta cggcaaacag cgcgattacg gtaacgaccc
    23881 gctgccgcgc ttacaggatt acgccgcgca gggtgccgaa gtgttgcatc tggtggatct
    23941 gaccggggca aaagatccgg ctaaacgtca aatcccgctg attaaaaccc tggtcgcggg
    24001 cgttaacgtt ccggtgcagg ttggcggcgg cgtgcgtagc gcagaagacg tggcggcgtt
    24061 actggaagcg ggcgttgcgc gcgtggtggt cggctccacc gcggtgaaat caccagatat
    24121 ggtgaaaggc tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg
    24181 tattgacgag caaggcaaca agcaggtggc agtcagcggc tggcaagaga actcgggcgt
    24241 ttcactggaa caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgctgtgtac
    24301 cgatatctcg cgcgacggca cgctggcagg ttctaacgtc tctttatatg aagaagtgtg
    24361 cgccagatat ccgcaggtgg catttcagtc atccggcggc attggcgaca ttgatgatgt
    24421 ggctgccctg cgtggcactg gcgtgcgcgg cgtaatagtt ggtcgggcat tactggaagg
    24481 taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac gcataattcc atgtctcgac
    24541 gttcgtgatg gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat
    24601 atcgtgccgc tggcaaaacg ctacgctgaa gaaggcgctg acgaactggt gttctacgat
    24661 atcaccgctt ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa
    24721 gtgatcgaca ttccgttctg tgtggcgggt gggattaaat cgctggatga cgccgccaaa
    24781 attctttcct ttggcgcgga taaaatttcc atcaactccc ctgcgctggc ggacccgacg
    24841 ttaattactc gcctggcgga tcgatttggc gtgcagtgta ttgtggtcgg tattgatacc
    24901 tggtacgacg ctgaaaccgg taaatatcat gtgaatcaat ataccggcga tgaaggccgc
    24961 acccgcgtca ctcagtggga aacgctcgac tgggtacagg aagtgcaaaa acgcggtgcc
    25021 ggagaaatcg tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgacctcgaa
    25081 caactgaaaa aagtgcgtga agtttgccac gtcccgctga ttgcttccgg tggcgcgggc
    25141 accatggaac acttcctcga agccttccgc gatgccgacg ttgacggcgc gctggcagct
    25201 tccgtattcc acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag
    25261 ggcgtggaga tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa
    25321 ccgacggact tatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg
    25381 gctatatgaa cccgaaagct ctggacaaaa ccatcgaaag cggcaaagtc accttcttct
    25441 cgcgcactaa acagcgacta tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag
    25501 taaatattgc gccggactgc gacaacgaca cgctactggt actggcgaat cctatcggac
    25561 caacctgcca taaaggcacc agcagctgct tcggcgaaac tgctcaccag tggctgtttc
    25621 tgtatcaact ggaacaactg ctcgccgaac gcaaatctgc cgacccggaa acctcttaca
    25681 ctgccaaact gtatgccagc ggcaccaaac gcattgcgca gaaagtgggc gaagaaggcg
    25741 tggaaaccgc actggccgcg actgtacatg accgctttga gctcaccaac gaggcgtctg
    25801 atttgatgta tcacctgctg gtgctgttgc aagatcagga tctggattta acgacggtga
    25861 ttgagaacct gcgtaaacgg catcagtgag ttgcggggta agcggatgca atattgttgt
    25921 cgcatccggc aaaaaaaacg ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
    25981 aaagattact tcgcgttgta attacgcaga gcgttacgcc ccagcacaat ccccgcacca
    26041 accatgccgc ccagcagcac cgccagaatc aaggtgattg cctttttcgg actatcgcga
    26101 cgaataggta acgtcggttt catcacataa cggtaagcat gaatatcaag atcatcaact
    26161 tttaagcttt cgatatccag caggttttga cgagtctgat agtagtttgg tgagaacacc
    26221 aacggacggg tcgcctcatg cttaatcatc gactccagcg cttcgctccc caaaaggaac
    26281 aacgtatctt gtgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga
    26341 ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg
    26401 actacttcct gagttttcag agaatcctgc aagtttttca tacgcagcgc gatgttgtct
    26461 ttcaggtctt tttccagttc ttcgtttacc tggtcgtcaa cttgctggat atacttcgcc
    26521 agctgcttct gcgcaccttc agctgtttca cccacataag aaactgctaa aggtaacgac
    26581 tggtttttaa cggtaggctc aatggtcagc ttctctgctt cttcctggtt atccagcgtt
    26641 tccgctaatg ctgagaatgc agtgctgtaa cgtccgataa gcgaagcctg aatgtcggag
    26701 actttcggta cagccgatcc gtagatcaca ttcatcgcgt tggtataacc agcgatttgc
    26761 cccacatcag gttgagtaac gatagctgtg gaagtccact tttctttggc aacggccaga
    26821 taccctatag ccagggcaat agcaacaatg acagagatga taattgtcat cttgccgcgc
    26881 cacaactgca ccagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa
    26941 acattattat tttctactct catcattacc ctaacggacg aaataatttt aagtcgccat
    27001 agtttagcga taatcgccat gaatgctata ggataaatga taaaaataat gaattattac
    27061 aaagggaaca taagagaatc agggcgaaaa tcgccctgat aacaggatgt tagtcactgc
    27121 caaagagatc gcgggtgtag actttgtctg ccacatcctt aagctcttct gccatacggt
    27181 tggaaataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc aggcgagagt
    27241 tgaagaatga gtcttctttc attaccggtt cgtaaatgat tacttcaacg cctttcgcct
    27301 tgatacgctt cataatcccc tgaatggaag acgcacggaa gttatctgac ccgctcttca
    27361 taatcagacg ataaataccc accacttgcg gtttgcgtga caaaatggca tcggcaataa
    27421 agtctttacg cgtgcggttt gcatcgacaa ttgccgagat caggttattc ggcacagact
    27481 ggtagttcgc cagtaactgc ttggtatctt tcggcagaca ataaccacca taaccaaacg
    27541 acggattgtt gtaatggttg ccaatgcgtg ggtcgagaca aacgccttcg ataatctggc
    27601 gagtattcag ccctaaactt tctgcatagc tatccagttc attaaagtat gctacgcgca
    27661 tcgccagata ggtattagca aacagtttaa tcgcttctgc ttcggttgaa tcggtgaaca
    27721 gggtcgggat attttgctta atcgcgcctt cctgtaataa cgcagcaaaa cgttctgcgc
    27781 gttccgaacg ctcaccgatt acaatacgtg acggatgaag gttatcgtaa agagctttac
    27841 cttcacggag aaattccgga gagaaaataa ggttatcgat acccagacgt tctttaattg
    27901 atttggtgaa acctaccgga attgtagatt taatcaccat taccgcgttg ggattaattt
    27961 ctgtcacatc gcgaatgacc gcttccacgc ttgaggtatt aaaataattt gttttcgggt
    28021 cataatcggt aggcgtggca ataataacgt aatcagcatt tttatacgcg tcatacttat
    28081 ctgtcgtagc gcggaaattg agatctttag tcgccagata ctcttcaatc tccttatcaa
    28141 caatcggtga ctgcttcttg ttaagcatgt caactttggc ctgaacgata tccagtgcaa
    28201 ccacttcgtg gttttgcgca atcagaatac catttgaaag accaacataa cctgttcctg
    28261 aaattgttat tttcatagtc gtttagctta gcaatattaa cttgtatagt tatatgtaca
    28321 gttgattagg aaagttgtca atataacagt accaaaaaaa ccttattata atgatatatt
    28381 aagaatatta ttatagtgac tacgacaaag agagccagaa attgaatact ttaatgtaaa
    28441 atcaatcaac actcaacaaa taactgaaat gttcctgatt tatatcgtca gagaattttc
    28501 ttgcaaa
//
LOCUS       DAZGCQ010000062        28190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000062 DAZGCQ010000000
VERSION     DAZGCQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.062"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(155..2728)
                     /gene="clpB"
                     /locus_tag="ACHK07_002963"
     CDS             complement(155..2728)
                     /gene="clpB"
                     /locus_tag="ACHK07_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO8103265.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2858..3589)
                     /gene="yfiH"
                     /locus_tag="ACHK07_002964"
     CDS             complement(2858..3589)
                     /gene="yfiH"
                     /locus_tag="ACHK07_002964"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO8103266.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3586..4566)
                     /gene="rluD"
                     /locus_tag="ACHK07_002965"
     CDS             complement(3586..4566)
                     /gene="rluD"
                     /locus_tag="ACHK07_002965"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO8103267.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            4701..5438
                     /gene="bamD"
                     /locus_tag="ACHK07_002966"
     CDS             4701..5438
                     /gene="bamD"
                     /locus_tag="ACHK07_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO8103268.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5709..6050
                     /gene="raiA"
                     /locus_tag="ACHK07_002967"
     CDS             5709..6050
                     /gene="raiA"
                     /locus_tag="ACHK07_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO8103269.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6153..6278
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6154..6201
                     /gene="pheL"
                     /locus_tag="ACHK07_002968"
     CDS             6154..6201
                     /gene="pheL"
                     /locus_tag="ACHK07_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO8103270.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6300..7460
                     /gene="pheA"
                     /locus_tag="ACHK07_002969"
     CDS             6300..7460
                     /gene="pheA"
                     /locus_tag="ACHK07_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO8103271.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7503..8624)
                     /gene="tyrA"
                     /locus_tag="ACHK07_002970"
     CDS             complement(7503..8624)
                     /gene="tyrA"
                     /locus_tag="ACHK07_002970"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO8103272.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8635..9705)
                     /gene="aroF"
                     /locus_tag="ACHK07_002971"
     CDS             complement(8635..9705)
                     /gene="aroF"
                     /locus_tag="ACHK07_002971"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO8103273.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9915..10280
                     /gene="yfiL"
                     /locus_tag="ACHK07_002972"
     CDS             9915..10280
                     /gene="yfiL"
                     /locus_tag="ACHK07_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO8103274.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10430..10948
                     /gene="yfiR"
                     /locus_tag="ACHK07_002973"
     CDS             10430..10948
                     /gene="yfiR"
                     /locus_tag="ACHK07_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO8103275.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10938..12164
                     /gene="dgcN"
                     /locus_tag="ACHK07_002974"
     CDS             10938..12164
                     /gene="dgcN"
                     /locus_tag="ACHK07_002974"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO8103276.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12180..12662
                     /gene="yfiB"
                     /locus_tag="ACHK07_002975"
     CDS             12180..12662
                     /gene="yfiB"
                     /locus_tag="ACHK07_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8103277.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12739..13086)
                     /gene="rplS"
                     /locus_tag="ACHK07_002976"
     CDS             complement(12739..13086)
                     /gene="rplS"
                     /locus_tag="ACHK07_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO8103278.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13128..13895)
                     /gene="trmD"
                     /locus_tag="ACHK07_002977"
     CDS             complement(13128..13895)
                     /gene="trmD"
                     /locus_tag="ACHK07_002977"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO8103279.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13926..14474)
                     /gene="rimM"
                     /locus_tag="ACHK07_002978"
     CDS             complement(13926..14474)
                     /gene="rimM"
                     /locus_tag="ACHK07_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO8103280.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14493..14741)
                     /gene="rpsP"
                     /locus_tag="ACHK07_002979"
     CDS             complement(14493..14741)
                     /gene="rpsP"
                     /locus_tag="ACHK07_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO8103281.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14878..16239)
                     /gene="ffh"
                     /locus_tag="ACHK07_002980"
     CDS             complement(14878..16239)
                     /gene="ffh"
                     /locus_tag="ACHK07_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO8103282.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16406..17197
                     /gene="ypjD"
                     /locus_tag="ACHK07_002981"
     CDS             16406..17197
                     /gene="ypjD"
                     /locus_tag="ACHK07_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO8103283.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17218..18504
                     /gene="yfjD"
                     /locus_tag="ACHK07_002982"
     CDS             17218..18504
                     /gene="yfjD"
                     /locus_tag="ACHK07_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO8103284.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRIMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18559..19152)
                     /gene="grpE"
                     /locus_tag="ACHK07_002983"
     CDS             complement(18559..19152)
                     /gene="grpE"
                     /locus_tag="ACHK07_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO8103285.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19275..20153
                     /gene="nadK"
                     /locus_tag="ACHK07_002984"
     CDS             19275..20153
                     /gene="nadK"
                     /locus_tag="ACHK07_002984"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO8103286.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNILSTKLGWSKKLF"
     gene            20239..21900
                     /gene="recN"
                     /locus_tag="ACHK07_002985"
     CDS             20239..21900
                     /gene="recN"
                     /locus_tag="ACHK07_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO8103287.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22049..22390
                     /gene="bamE"
                     /locus_tag="ACHK07_002986"
     CDS             22049..22390
                     /gene="bamE"
                     /locus_tag="ACHK07_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO8103288.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22452..22742)
                     /gene="yfjF"
                     /locus_tag="ACHK07_002987"
     CDS             complement(22452..22742)
                     /gene="yfjF"
                     /locus_tag="ACHK07_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO8103289.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22732..23169)
                     /gene="ratA"
                     /locus_tag="ACHK07_002988"
     CDS             complement(22732..23169)
                     /gene="ratA"
                     /locus_tag="ACHK07_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO8103290.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23340..23822
                     /gene="smpB"
                     /locus_tag="ACHK07_002989"
     CDS             23340..23822
                     /gene="smpB"
                     /locus_tag="ACHK07_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO8103291.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24037..24399
                     /gene="ssrA"
                     /locus_tag="ACHK07_002990"
     tmRNA           24037..24399
                     /gene="ssrA"
                     /locus_tag="ACHK07_002990"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24583..25776
                     /locus_tag="ACHK07_002991"
                     /pseudo
     CDS             24583..25776
                     /locus_tag="ACHK07_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
     gene            complement(25779..26624)
                     /locus_tag="ACHK07_002992"
     CDS             complement(25779..26624)
                     /locus_tag="ACHK07_002992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8103292.1"
                     /translation="MWTLPLPDDADRMSELLTALTYKNGNPKYALTQDEKDKIETIYT
                     LYHDRKATPCAEFLAEEISDETQNAIKTAYSEVQINGRLSTLRSRILLSSKKCPLCGI
                     ESSTDLDHYLPESIYKCLAVYSRNLIPTCHKCNNKKRAGVAENGVGFIHVYYFDEPDG
                     VFFVANTEMIDGALVIDFNINMIEGMTREVYDSLCFQNRVVNLNERLKMEANDFLGGL
                     YTSLEETYNSAGQEGLRLFLKRTRDDYTRRYGLNHWRRALIDSLSDNVEFLDAGFITA
                     LGTPE"
     gene            complement(26630..>28190)
                     /locus_tag="ACHK07_002993"
     CDS             complement(26630..>28190)
                     /locus_tag="ACHK07_002993"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8103293.1"
                     /translation="WNDFSFITKYHLYITTKLTGLDEIEYAGVVKIIKKDQSEADYYM
                     VEEGEFSYLSDEFCSVGQSLDYYGRLSELGSEICEGILTSLRDINMFDYVSEGFKNER
                     AWERSIMRDLNNDDDIFILPKFIIKNQFSAIPSIDMKFSFMYSGMINTLSFDFDSIQY
                     GYFESEKLPCRMSVIVGRNGCGKSTTLARLSRVAYSSTQDRKKEQIAKIGEILPEGLG
                     FPKIINLSYSAFDSFQIPGCTFKEKKQLRQDILDGKGRYIYCGVRDVGAELDYVLANV
                     DENNMDIEFITLDRQERTILKPLEVLSEEFYGVLIKIHKDSDKWNLFNKVMMKLREEV
                     SLSEIIDEIFKDDFILKSKEYFHRLSTGHKFVLHSMAHILLYIEKRCLVLFDEPETHL
                     HPPLLAVLMSALRIVLNEVDAFAIVATHSPVVVQETLSKNVNVITRNGEFTDFQRPEI
                     ETFGENIGAITSSVFSLTTQITDYHDELDKLIIEYKNNNNPLDDIEKCFDTGLSTQAR
                     SYVISKLAVKG"
BASE COUNT         7371 a         7280 c         6378 g         7161 t
ORIGIN      
        1 agtggtggcg cattataggg agttattccg gcctgacaag cgaaaaatat aaaaacttta
       61 tcgttcgctc acttttcagg caaaacatct taaatatagt cttttccgtc taacttatag
      121 acaaaaacga gccccgaagg gctcgtttta tcatttactg gacggcgaca atccggtctt
      181 cattaacttc caggcgaatc actttacccg gaaccaattc accagacagt atttgctgtg
      241 ccagcgggtt ttcgatctgc tgctgaattg cacgtttcag aggacgtgca ccatagaccg
      301 gatcgtaacc gttctcgctc agcagtttca gcgcctcgtc agaaatgtgg atttcataac
      361 cacgttcttc cagacgttta tacagacgtt tcaactgaat ctgcgcaatc gacgcaatgt
      421 gctgttcacc cagcggatgg aagaccacca cttcatcgat acggttaatg aattccggac
      481 ggaagttatg gctaaccaca ccgagcacca gctctttcat gtgcgcataa tccagttcac
      541 cgaagcgttc ctgaatcaaa tcggaaccga ggttagaggt cataatgaca accgtattac
      601 ggaagtcgac cgttctcccc tgcccgtcag tcagacgccc atcatccagt acctgcaaca
      661 gaatgttgaa gacatccgga tgcgcttttt ccacttcatc cagcaggata acggaatacg
      721 gacgacgacg cacagcttcg gtcaggtagc caccttcttc ataaccgaca tatcccggag
      781 gcgcaccaac cagacgagac accgagtgtt tctccataaa ctcggacata tcgatacgga
      841 ccatcgcctc gtcgctatca aacataaagt tcgccagcgc cttacaaagc tctgttttcc
      901 ccacaccagt tgggccgagg aacaggaatg aaccaatcgg gcgatttgga tccgccagcc
      961 ccgcacggct acgacgaata gcgttagata ccgcatcaac cgcttcgttc tgaccaatta
     1021 cgcgatggtg cagttcttgc tccatacgca gcagtttttc gcgctcgctt tccatcatgc
     1081 gagaaaccgg aatccccgtc caacgcgcca gcacttcagc aatttcggcg tcggtcactt
     1141 tattacgcaa tagacgcata gttttgcctt cgagctgcgt tgcggcttcc agttgctttt
     1201 ccagttccgg gattttgccg tattgcagtt cagacatccg cgccaggtcc cccacacggc
     1261 gagcctgttc aatagcgatt ttcgcctgtt ccagttccgc tttaatggtc tgcgtaccag
     1321 aaagcgatgc cttctctgct ttccactctt cttctaactc ggagtactga cgttctttgt
     1381 cgctcagttc ttcgttgagc atatccaggc gttttttact ggcttcatca gactctttca
     1441 ttaacgcctg ttgttccagt ttgagctgga tgatacgacg atcgagtcgg tcgagttctt
     1501 ctggttttga gtcaatttgc atacgaatgc tggatgctgc ttcatcgatc aggtcgatag
     1561 ctttatccgg cagctgacgg tcagcaatgt agcgatgaga caacgtcgcc gctgcaacaa
     1621 ttgccgggtc agtaatttgc acatggtggt gcaattcgta acgttctttc aggccacgca
     1681 gaatcgcaat ggtatcttca acagaaggct cggcaacaaa cactttctgg aaacgacgtt
     1741 ccagcgcagc atctttttcg atgtactggc gatattcgtc aagcgtagtg gcacctacgc
     1801 agtgcaattc accacgcgcc agcgccggtt tcagcatgtt tccggcgtcc attgcgccat
     1861 cggctttacc cgcgccgacc atggtatgta attcgtcgat aaacaggatg acgttgcctt
     1921 cctgtttggc aagatcgtta agcacgcctt ttaaacgttc ttcaaactca ccgcgatatt
     1981 tcgccccagc caccagcgcg cccatatcca gcgccagtac ccggcggcct ttcaaccctt
     2041 ccggcacttc gccgttgata atacgctgcg ccagaccttc aacgatggca gttttaccga
     2101 cgccgggttc accaatcagt accgggttat ttttagtacg acgttgcagc acctgaatgg
     2161 tacggcgaat ttcttcatca cgaccaatca ccggatcgag tttgccctgt tcggctcgtt
     2221 cggtaaggtc gatggtatat tttttcaaag cctgacgttg gtcttcagca ccttgatcgt
     2281 tcacgctttc acctccacgc atttgttcaa tcgcttgagt aatgttggcg gtggtcgccc
     2341 ctgctgcttt caggatgtcg gccagcgtgc cgcgagactc aagtgccgcc agaacgaaca
     2401 gttctgacga gataaagtta tcaccacgtt tttgcgccag cttgtcgcaa agattaagaa
     2461 cgcgcaccag atcctgtgat ggctggacat caccaccagt accttcaacc tgcggtaaac
     2521 gatttaatgc ctgattgata tctgtgcgca actggccagc atttatgcca gctgatgtta
     2581 acaaaggact aaccgacccc ccttcctgat tcagcagggc gctcattaaa tgaagtggtt
     2641 cgatgaattg gttgtcgtgc ccgagtgcaa gtgattgggc atcggcaaga gcaagctgga
     2701 atttattagt aagacgatcc agacgcataa ctcctcccat aacggatcaa agttgctact
     2761 ggagattaaa tgaggtcatc cctcaattat tcaaggttat tgaccagatt aatgtgaaaa
     2821 gaaaatcacg cgtaccggat cgtcttgatt ctttaggtta tatcagccaa atgaaacttg
     2881 ccatacgccc ggtggtcttg tcgcgacgat aagagaagaa agtctcattt tccgtatatg
     2941 tacaacggtc gccgccgaaa atttgttcaa caccaacgct cgccagacgc tgccgggcaa
     3001 gcagataaat atccgccaga tacttatcac catgctgaat gaaagctgta ctcgctttgg
     3061 cgtctgctgc cataaacgcc tcgcgaacct ccgcccccac ttcgaacgcg cgtggaccaa
     3121 ttgccggccc taaccaggcg agaatatttt ccggattatc agcaaaacag gaaactgtct
     3181 cttccagcac gcctgcgcac agtccacgcc agccagcatg agcggcggcg acttccgttc
     3241 ccgctcgatt gcaaaacagc acagggaggc agtcggcagt catcactgcg caaaccgtgc
     3301 caggcgtatt gctataagag gcatctgctc gttttgaggc ataaggttcg ccagtaagct
     3361 taagcacatc tttgccgtgt acctgctcaa gccagaccgg tttagaaggc aaattgcccg
     3421 cagcaaaaag tcgcttgcga ttctcctcaa catgatccgg gttatcgcca caatgggcac
     3481 cgaggttgag cgagtcatac gggggcaagc tcacgccgcc gatacgagta gagctacaag
     3541 ccgcaacacc ttttggcagc ggccactgcg ggacaatcag cttactcata accagtccac
     3601 ttcatcctta tgttcttcga aatcggcacg catcgcctca atcagctcca ccatatcttg
     3661 tggaataggc gcatgccatt ccatttcgat gccggagatc ggatgataaa gacgcagcat
     3721 ggttgcatga agcgcctggc ggtcaaactt acgcagcgtg gagataaacg cttccgaagc
     3781 accttttggc ggacgcggac ggccaccata aaccggatcg cccaccagcg gatgagtgat
     3841 atgggccata tgcacgcgga tctggtgcgt acgtccagtt tccagacgca accgcagacg
     3901 cgtgtgcaca cggaagtgtt ccatgatgcg atagtgagtc accgctggtt tgcccatcgg
     3961 atgcaccgcc atgtgagtac gtttggtcgg gtggcgactg attggctcat ccaccgtacc
     4021 gccagcggtc atatgaccaa tcgccaccgc ttcgtactca cgagtaattt cacgcctttg
     4081 caaagattcg actaaacgcg tctgagccgg aacggttttt gccacaacca tcaggccagt
     4141 ggtgtcttta tccagacgat ggacgatgcc cgcacgcggt acatcggcaa taggcggata
     4201 ataatgaagc aacgcattca gtaccgtgcc atccgggtta cccgcgccag gatgaaccac
     4261 caggtcgcgc ggtttattaa tgacgatgat gtcttcatct tcatagacga tatccagcgg
     4321 gatatcctgc ggttcaaaac gcgcttcttc ttcaatctct gcgttgatgg caacctgctc
     4381 gccacccaat actttttctt tcggcttatc acaaagtttg ccgttaacca gcacgcgctg
     4441 gtcgaggatc cattctttta ttcgcgaacg tgaataatcc gggaacattt cggccaaagc
     4501 ctgatctaag cgttgaccga gttggttttc ggacaccgtt gcagtgagct gtactcgttg
     4561 tgccatatat actgcttctt cgtttatcgt tggggtttta cggctttgcc gtttaatata
     4621 gtgtgctatt gtagctggtc ttaaccggga gcaggaacag agaatctccc gtattacatt
     4681 ttgaggaaag tcaaaacgtc atgacgcgca tgaaatatct ggtggcagcc gccacactaa
     4741 gcctgttttt ggcgggttgc tcggggtcaa aggaagaagt acctgataat ccgccaaatg
     4801 aaatttacgc gactgcacaa caaaagctgc aggacggtaa ctggagacag gcaataacgc
     4861 aactggaagc gttagataat cgctatccgt ttggtccgta ttcgcagcag gtgcagctgg
     4921 atctcatcta cgcctactat aaaaacgccg atttgccgtt agcgcaggct gccatcgatc
     4981 gttttattcg ccttaacccg acccatccga atatcgatta tgtcatgtac atgcgtggcc
     5041 tgaccaatat ggcgctggat gacagtgcgc tgcaagggtt ctttggcgtt gaccgtagcg
     5101 atcgagatcc tcaacatgca cgagctgcgt ttagtgactt ttccaaactg gtgcgcggct
     5161 atccaaacag tcagtacacc accgatgcca ccaaacgtct ggtattcctg aaagatcgtc
     5221 tggcgaaata tgaatactcc gtggccgagt actatacaga acgtggcgca tgggttgccg
     5281 tcgttaaccg cgtagaaggc atgttgcgcg actacccgga tacccaggct acgcgtgatg
     5341 cgctgccgct gatggaaaat gcataccgtc agatgcagat gaatgcgcaa gctgaaaaag
     5401 tagcgaaaat catcgccgca aacagcagca atacataaca gaaacctgaa acacaaaacg
     5461 gcagcccttg agctgccgtt tttttattct gtcagttgtg aaactgaagc gatttagtca
     5521 ctatcgatct catcaaatat ggctcgcttt gagatattcc tcaagtaaaa aaacacctct
     5581 tcctgcgatt tctcacaaaa aagattcgtt gacaaaaagt gacaaaatta tgagatttcc
     5641 atcacacatt ttgacatcag gaacggtatg ctgaattcac caagacggga agacaagagg
     5701 taaaatttat gacaatgaac attaccagca aacaaatgga aattactccg gccatccgcc
     5761 aacatgtcgc agaccgtctc gccaaactgg aaaaatggca aacacatctg attaatccac
     5821 atatcattct gtccaaagag ccacaagggt ttgttgctga cgccacaatc aatacaccta
     5881 acggcgttct ggttgccagt ggtaaacatg aagatatgta caccgcaatt aacgaattga
     5941 tcaacaagct ggaacggcag ctcaataaac tgcagcacaa aggcgaagca cgtcgtgccg
     6001 caacatcggt gaaagacgcc aacttcgtcg aagaagttga agaagagtag tcctttatat
     6061 tgagtgtatc gccaacgcgc cttcgggcgc gttttttgtt gacagcgtga aaacagtacg
     6121 ggtactgtac taaagtcact taaggaaaca aacatgaaac atacaccgtt tttcttcgca
     6181 ttctttttta ccttcccctg aatgggaggc gtttcgtcgt gtgaaacaga atgcgaagac
     6241 gaacaataag gcctcccaaa tcggggggcc ttttttattg ataacaaaaa ggcaacacta
     6301 tgacatcgga aaacccgtta ctggcgctgc gagagaaaat cagcgcgctg gatgaaaaat
     6361 tattagcatt actcgcagag cggcgcgaac tggccgtcga ggtgggaaaa gccaaactgc
     6421 tctcgcatcg cccggtacgt gatattgatc gtgaacgcga tttactggaa agattaatta
     6481 cgctcggtaa agcgcaccat ctggacgccc attacattac tcgcctgttc cagctcatca
     6541 ttgaagattc cgtattaact cagcaggctt tgctccaaca acatctcaat aaaattaatc
     6601 cgcactcagc acgcatcgct tttctcggcc ccaaaggttc ttattcccat cttgcggcgc
     6661 gccagtatgc tgcgcgtcac tttgagcaat tcattgaaag tggctgcgcc aaatttgccg
     6721 atatttttaa tcaggtggaa accggccagg ccgactatgc cgtcgtaccg attgaaaata
     6781 ccagctccgg tgccataaat gacgtttacg atctgctgca acataccagc ttgtcgattg
     6841 ttggcgagat gacgttaact atcgaccatt gtttgttagt ctccggcacg actgatttat
     6901 ccaccatcaa tacggtctac agccatccgc agccattcca gcaatgcagc aaattcctta
     6961 atcgttatcc gcactggaag attgaatata ccgaaagtac gtctgcggca atggaaaagg
     7021 ttgcacaggc aaaatcaccg catgttgctg cgttgggaag cgaagctggc ggcactttgt
     7081 acggtttgca ggtactggag cggattgaag cgaatcagcg acaaaacttc acccgatttg
     7141 tggtgttggc gcgtaaagcc attaacgtgt ctgatcaggt tccggcgaaa acgacgttgt
     7201 taatggcgac cgggcaacaa gccggtgcgc tggttgaagc gttgctggta ctgcgcaacc
     7261 acaatctgat tatgacccgt ctggaatcac gcccgattca cggtaatcca tgggaagaga
     7321 tgttttatct ggatattcag gccaatcttg aatcagcgga aatgcaaaaa gcattgaaag
     7381 agttagggga aatcacccgt tcaatgaagg tattgggctg ttacccaagt gagaacgtag
     7441 tgcctgttga tccaacctga tgaaaaggtg ccggatgatg tgaatcatcc ggcactggat
     7501 tattactggc gattgtcatt cgcctgacgc aataacacgc ggctttcact ctgaaaacgc
     7561 tgtgcgtaat cgccgaacca gtgctccacc ttgcggaaac tgtcaataaa cgcctgctta
     7621 tcgccctgct ccagcaactc aatcgcctcg ccgaaacgct tatagtaacg tttgattaac
     7681 gccagattac gctctgacga cataatgatg tcggcataaa gctgcggatc ctgagcaaac
     7741 agtcgcccga ccatcgccag ctcaaggcgg taaatcggcg aagagagcgc cagaagttgc
     7801 tcaagctgaa cattttcttc tgccaggtgc agcccgtaag caaaagtagc aaagtggcgc
     7861 agtgcctgaa taaacgccat attctgatcg tgctcgacag cgctaatacg atgcaaccga
     7921 gcgccccaga cctgaatttg ctccagaaac cattggtatg cttccggctt acgtccatca
     7981 caccagacca caacttgctt tgccaggcta ccgctgtccg ggccgaacat cgggtgtaac
     8041 cccagtaccg ggccatcgtg cgccgccagc atggcctgta atggtccatt tttcactgat
     8101 gccagatcaa ccagaataca atctttcggt aaaggcggta atttgccgat aacttgctca
     8161 gtaacgtgga ttggcacact aacaatcacc attccggcat cggcaacaat atcagccgct
     8221 cgatcccagt catgttgctc cagaatccgc acctgataac ccgatagtgt cagcatcttc
     8281 tcgaacaggc gtcccatctg accgccgccg ccaacgataa ctaccgggcg taacgcagga
     8341 caaagtgttt taaatccttt gtcgttttca ctggagtaag attcacgcat cacccgacgc
     8401 aaaacatcct caatcagatc tggcggtaca cccagagctt ccgcctctgc acgacgcgag
     8461 gccaacatag atgcctcgcg ctccggaaca taaataggca gtccaaagcg gcttttcacc
     8521 tcgcccactt cagcaaccag ttccagacgc ttcgctaata aattcagcag cgctttatcg
     8581 acttcatcaa tttgatcgcg taatgcggtc aattcagcaa ccataataaa cctcttaagc
     8641 cacgcgagcc gtcagctgcc cgttcagatc ctgatgaatt tcacgcagca aggcatcggt
     8701 catttcccag ctaatgcagg catcggttac ggatacaccg tatttcattt cactgcgcgg
     8761 ttgctcggaa gactgattgc cctcgtggat attactttcg atcatcagac caataattga
     8821 gcgattgcca tctttgattt gagcaaccac ggattctgcc accgcaggct gacggcgata
     8881 atctttattg gaattaccgt ggctgcaatc taccatcaga gacgggcgca gtcccgcctg
     8941 ttccatctct ttttcacatt gcgcaacatc cgcagggcta tagttcggcg ctttaccacc
     9001 gcgcaggatc acatgtccgt ccggattccc ctgagtttgt agcaacgcaa cctgacctgc
     9061 ctggttaatg ccaacaaaac ggtgcggctg ggcggcggcg cgcatagcgt taattgctgt
     9121 tgccagactg ccgtcggtgc cgtttttaaa accaaccggc atggaaagcc cggaggccat
     9181 ttcacggtga gtttgcgatt ccgttgtacg agcaccaatt gctgaccagc taaacaaatc
     9241 gcccaggtat tgcgggctat tcggatctaa cgcttccgtc gccagtggca gtcccatatt
     9301 caccagctca agcagcaatt tacgcgcgat ctgcagcccg gcttctacat caaaagagcc
     9361 atccatatgg ggatcgttaa ttaacccttt ccagccgaca gtggtacggg gtttttcaaa
     9421 atagacgcgc attaccagat agaggctatc gctgacctct gcggcaaggg ctttaaatcg
     9481 acgagcatat tccagagcag tttccggatc atgaatggaa caaggaccac atactaccag
     9541 cagacgagga tcgcgcccgg cgataatatc tgaaatggtt ttacgcgagt cagcaatctg
     9601 ggcttcttgt tgcaggctta atggaaaagc ggccttcagt tgtgccggag tcattaaaac
     9661 ctgttcgtcg gtaatatgta cgttattcag cgcgtctttt tgcatgatgg cgatcctgtt
     9721 tatgctcgtt tgcgatagtt gatcctcagc gaggatgacg taacgataac acataaagta
     9781 aagttttcaa tccatatttc gtacattttt ctttacagaa gcaatttatt cgcgctttca
     9841 acccttgatt ctgtatagat aaatttacac cacctttgaa aacaatccgc tatgctttga
     9901 aaaaggagaa agaaatgatg aaaaagttta tcgccccctt gttggcttta ctggttagcg
     9961 gatgccagat tgatccttat actcacgcgc caaccttgac cagcaccgac tggtatgatg
    10021 ttggtatgga agatgcgata tcgggcagcg ccataaaaga tgacgatgca tttagcgatt
    10081 cacaggcgga tcgcggtcta taccttaaag gatatgccga aggacaaaag aaaacttgcc
    10141 agaccgattt tacttatgcc cgaggacttt ccggtaaaag ctttcctgcc agctgcaaca
    10201 atgttgaaaa tgccagccaa ctgcatgaag tatggcaaaa aggggctgat gaaaatgcca
    10261 gcgcaatacg attaaattaa ttaaaacccc acaaataaat taaacataag attttactca
    10321 ttatgtttta tttctcacaa tgatgacgac cgcatgaaat aatgatattt taatgagcat
    10381 tctgaaaaaa tatcgatgca tttcgagcga agatggtggg gatccctgaa tgcgtttttc
    10441 tcaccgactg ttcttactgc tgattctcct gctgacggga gcaccaatac tcgctcagga
    10501 gccttctgac gttgcgaaaa acgtgcgtat gatggtttcc ggtattgtca gttacacccg
    10561 ctggcctgcg ctatcagggc caccaaagtt atgtatcttt tcatcttcac gttttagtac
    10621 cgccctacaa gagaatgccg cgacatcttt gccttatctg cctgtcatca ttcacactca
    10681 acaagaggcg atgatttcag gctgcaatgg tttttatttt ggcaacgagt caccgacatt
    10741 ccagatggaa ttaaccgaac aatatccgtc aaaggcgtta ttattaattg ccgaacaaaa
    10801 taccgagtgc attattggta gcgccttttg tctgatcatc cacaataatg acgtcagatt
    10861 tgccgtaaac ctggatgcct tatcgcgtag cggtgtaaag gtcaacccgg atgtcttaat
    10921 gctcgcacgg aagaaaaatg atggataacg ataattctct taataagcgc cccacgttta
    10981 aaagagcatt acgcaacatc agtatgacca gcatatttat cactatgatg ctgatctggt
    11041 tgctgctttc cgtgacctcg gtgctgaccc tgaaacagta cgcgcaaaaa aacctggcac
    11101 tgacagcagc aacaatgact tacagtctgg aagcagctgt cgtttttgcc gatggccctg
    11161 cagcaactga aacactggca gcgctgggcc agcaagggca attttcaact gcagaagtac
    11221 gtgataagca gcaaaatatt ctggcatcct ggcattacac ccgtaaggat ccaggcgata
    11281 ctttcagcaa tttcataagc cactggctct tccctgcccc catcattcag ccgattcgtc
    11341 acaatggtga aaccattggc gaagtacgct taaccgctcg cgacagttca atcagccatt
    11401 ttatctggtt ttcgctcgcc gtactgaccg gttgtattct gctggcatca ggaatcgcaa
    11461 ttaccctcac ccgccatttg cacaatggcc tggtggaagc actgaaaaat atcaccgatg
    11521 tcgtacatga tgtgcgttcc aaccgcaatt tttcccgacg agtttcggaa gaacgtatcg
    11581 ctgagtttca ccgcttcgct ctcgacttca acagtctgct ggatgaaatg gaagagtggc
    11641 agcttcgttt acaggccaaa aatgcgcagc ttctgcgtac cgcgctacat gacccattaa
    11701 ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa tacgttgatg aacaattccg
    11761 atgcccgaaa aacgtcggcg ttactatttc ttgatggcga taatttcaaa tatatcaatg
    11821 atacctgggg tcatgcgacg ggcgatagag tcttgattga aatcgcaaaa cggttagctg
    11881 aatttggcgg gctgcgacat aaagcatacc gcctgggcgg cgatgaattc gctatggtgc
    11941 tctatgatgt acagtcggaa tctgaagtgc agcagatatg ctcagcactg acacaaatct
    12001 ttaatctccc gtttgatctt cataatggcc atcagaccac catgacatta agcattggtt
    12061 acgcaatgac cattgcgcac gcttctgcgg aaaaattaca agagctggcc gatcacaata
    12121 tgtatcaggc caaacaccag cgtgccgaaa agctggtgag ataacaagga tatatcgata
    12181 tgataaagca cctggtagca cccctgattt tcacctcact aatactgact ggctgccagt
    12241 cccctcaggg aaagtttact cctgagcaag tcgccgctat gcaatcttat ggatttactg
    12301 aatccgccgg cgactggtcg ctgggcttat cagatgccat tctgttcgca aaaaatgact
    12361 acaaattgct cccggaaagc cagcaacaga tccaaaccat ggcagccaaa ttggcctcga
    12421 cagggctaac acatgcccgt atggatggac acaccgataa ctatggtgaa gacagttaca
    12481 acgaaggctt atcattgaaa cgggcgaatg tcgtggccga tgcatgggct atgggtggac
    12541 aaattccacg cagcaatctc accacacagg gtttaggaaa aaaatatccc atagccagta
    12601 acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc agtggtgatt actacccctt
    12661 aaaacacgag caaacagata aaaaaagggc cagccaattg gccagccctt cttaacagga
    12721 tgtcgcttaa gcgaaatctt agttaagacg ctctttgata cgagcagcct taccagtacg
    12781 ctcacgcagg tagtacagtt tagctttacg aacagcacca cgacgtttga cagaaatgct
    12841 gtcaactacc ggagagtgag tctggaagac acgctcaacg ccttcgccgt tggaaatttt
    12901 acgaacagtg aatgcagagt gcagaccgcg gttacgaata gcgataacca cgccctcgaa
    12961 tgcctgcaga cgttttttgg aaccttcaac aacccatact ttcacttcca cggtatcacc
    13021 cggacggaag gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct taataatgtt
    13081 gctcataatt taatctctta tcctgggtaa actgatatct cgggggctta cgccatccca
    13141 tcatgtttat gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa ccttgcttgc
    13201 tcttcagtca gagccaggtt ttccagaagt tcaggtcttc taagccaggt acggcccagc
    13261 gactgtttca aacgccagcg acgtatctcg gcatggttgc ccgacagtaa cactggcgga
    13321 acctccatcc cttctaacac ctcaggccgc gtatagtgcg ggcaatccag caatccttca
    13381 gcaaaggaat cttccgttgc cgaggcttca tgtcccagta ctcccggaat aaaccgggaa
    13441 acggagtcaa tcagcgtcat tgctggtaac tcaccaccac tgagaacgta atcgccgatt
    13501 gaccattctt cgtcaatttc ggtttggatc acgcgctcat ctataccttc gtagcgaccg
    13561 cacaccagaa tcaatttttg attcgttgcc agttcgctga cgcccgcttg atcaagcttg
    13621 cgtccctgtg gtgaaagata aatcaccttt gcgccttcac ccgccgcggc ttttgctgca
    13681 tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc gccgtaagga
    13741 cgatcgtcca cggtacggtg ccggtcatgc gtgaaatcgc gaggactcca gctctggatg
    13801 ctcagcaggc catttttaac tgcccggcca gttaccccgt aatcggtaat tgcgcggaac
    13861 atttcaggaa acaggctaat tatgccaatc cacatagcgc cgtcttttac cgtttatccg
    13921 gtggtttaaa aaccaggatc ccaatctact tcgattgaac gagtagtgag atcgactttc
    13981 ttgataacct gcccatcgag gaacggtacg agacgttcct tgataccaaa cgcatctttc
    14041 aggtttgcct taatgacgag aacgtcattg gatccggttt ccatcatatc gacgacttta
    14101 ccgagatcgt agccttcagt ggttactacc tggcagccca tcaggtcttt ccagtagtag
    14161 tcaccctctt caagctgagg cagctgcgat gaatccacga caatttcaca atttgtcagc
    14221 aggttcgccg catcacgatc gtcaacgcct ttcagcttga tgatcatgtc ctgattgtgg
    14281 tgcttccagc tttccagctg gacttgctgc cactgacccg ccttctggat aaaccagggc
    14341 tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa acactctgag ccacccacga
    14401 ataccgtaag acgaacccat ttttcccaaa acgatgggat caacaggtgc ttgcgcggtg
    14461 agttgtttgc tcatcatgac caccgtgaca gattaagctg ctttgtttac ttctttgatc
    14521 agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa cccagtgagc gatgcgatcc
    14581 agatccaggc gagtgccttc ttctttttcg ctagcgattg ggttgaagaa accaacgcgc
    14641 tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga caacaacctg gtagaacgga
    14701 cgctttttag cgccgtgacg tgctaaacga atagttacca taacatcctc ttgtgtgaat
    14761 aaaacaaccg gaccccatcg aggaacggag tccggtgtca tattaaaagc ccgaaaattt
    14821 tactgatttc tggagaaaat gcaatcagca gttaataact ctgtcgcata aggtcattta
    14881 gcgaccagga aatcccggcg gcatcatccc cttcatgctt ctcatcatct tcgccattcc
    14941 gcccttcttc attttcttca tcatgcgctg catgtcgtcg aactgtttca gaagacggtt
    15001 aacgtcctgc acctgcatac cgcaaccggc agcaatacgg cgtttacgcg aacctttgat
    15061 gatttctggc ttagcgcgct ctttcatcgt catcgagttg atgatggctt ccatacgcac
    15121 cagcacttta tcgtccatct gtgacttgac gttatccggg atctgcccca tgcccggcag
    15181 cttgcccatc agactagcca tgccgcccat atttttcatc tggcgcagtt gctcaagaaa
    15241 gtcgttgaga tcgaagccgt cgcctttttt tagcttgctg gctaatttct ctgcctgcgc
    15301 gcggtcaact ttgctttcga tatcttcgat cagcgacagt acgtcgccca tgccgagaat
    15361 acgcgacgcg atgcggtccg gatggaacgg ctccagcgcc tcagttttct cgccaacacc
    15421 gaggaacttg atcggtttgc cagtgatgtg acgaatagag agcgccgcac cgccgcgggc
    15481 atcgccgtcc actttggtca acactacgcc ggtcagcggt aacgcttcat tgaatgcttt
    15541 tgccgtattg gccgcatcct gaccggtcat ggcgtcaacc acaaacaggg tttcaaccgg
    15601 atttatggac gcatggactt gtttgatctc gtccatcatc gcttcgtcaa cgtgcagacg
    15661 accagcggta tccaccagca gcacgtcgta gaatttcagt ttggcttctt tcagcgccgc
    15721 gttaacgata tctaccggct tctgaccaac atcagaaggg aagaaatcaa cgcccacctg
    15781 ctctgcaagc gtctcaagct gtttgattgc cgctggacga taaacgtcgg cagaaaccac
    15841 cagcactttt ttcttgtgct tctcgcgcag gaacttaccg agcttaccga cactggtggt
    15901 tttaccggca ccttgcaggc ccgccatcag cacgaccgca ggcggttgtg ccgccagatt
    15961 cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg ttacggacga ttttgacgaa
    16021 ctcctgcccc ggcgtcaggc ttttattaac ttcatgacca accgctttct cttttacgcg
    16081 attgataaac tcacgcacta ccggcagagc gacgtccgcc tccagcagcg ccatgcgcac
    16141 ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt ccacggccac tgatattgcg
    16201 cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt gtctctcgcc tggggtggaa
    16261 acggttggcc gcaatcgcga cacatcatca gtattttgcc gcagtataac atgaaggcgt
    16321 ctttgttgtt atgcaacggt tggagcagcg ttcacctgac gctatactgc ttctctttct
    16381 tattgctcaa actgtcgaca ttactatgcc cgtttttgct ctgctcgcgc ttgtcgccta
    16441 ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg gctggcggcg
    16501 catggctatt atttctgcgg tcattgcgct ggtctgccac gcaatcgctc tggaagcccg
    16561 catcctgccc gacggtgata gcggacaaaa cctcagcctg ctgaacgttg gttcattggt
    16621 cagtttgatg atctgtacgg taatgaccat tgtggcttct cgcaatcgtg gctggttgct
    16681 gctacccatt gtctatgcct ttgcgcttat caacctggcg ctggcaacct tcatgcccaa
    16741 tgaatacatc acccatctgg aagctacacc tgggatgctg gtgcacattg gcttatcgct
    16801 cttttcctat gccacgctaa ttatcgccgc cctgtacgcg ctgcaactgg cgtggattga
    16861 ttaccaactg aagaacaaga agctggcgtt taaccaggaa atgccgccat tgatgagtat
    16921 cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg ctgctaacgc tcacgctttg
    16981 cactggcctg ttctacatgc acaacttatt tagcatggaa aatatcgaca aggctgtgct
    17041 ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg ggacattatc atgaaggatg
    17101 gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggtgca gtcattctga cactggccta
    17161 cttcggcagc cgaattgtcc agcagttaat cagctaaacc cagaaaagga gtttcccctg
    17221 gaacacattt ctactactac gttgatcatt attctgatca tcatggtggt catttcagcc
    17281 tatttttccg ggtccgaaac cggaatgatg accctcaacc gctatcgtct gcgacatatg
    17341 gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat tgctgcgtaa gccagaccgc
    17401 ctgataagcc tggtgttaat cggcaataac ctggtcaata ttcttgcctc cgcgctcggc
    17461 actattgttg ggatgcgttt gtacggcgat gcgggcgtgg caattgcgac tggtgtgctg
    17521 acctttgtgg tgctggtttt tgccgaagtg ttgccgaaaa ctatcgctgc gctatacccg
    17581 gaaaaagtcg cttatccgag tagtattctg ttagcaccgc tgcaaatttt gatgatgccg
    17641 ctggtctggt tgctgaacgc tatcacccgc atgttaatgc gcattatggg catcaaaacc
    17701 gatatcgtgg tcagcggctc tttgagcaaa gaagagttgc gcactatcgt gcacgaatcg
    17761 cgctcacaaa tttcccgtcg caatcaggat atgctgctgt cggtgctcga tctggaaaaa
    17821 atgaccgttg atgacatcat ggtgccgcgc agtgaaatta tcggtattga tatcaacgat
    17881 gactggaaat ctattctgcg ccaactctcc cactcacctc acgggcgcat cgtgctctac
    17941 cgtgattcgc tggacgacgc catcagtatg ctgcgagtac gtgaagcctg gcggttgatg
    18001 tcggagaaaa aagagttcac caaagaaacc atgctgcgcg ccgcggacga gatctatttt
    18061 gtgccggaag gtacgccgct cagcacgcag ttggtaaagt ttcagcgcaa caaaaagaaa
    18121 gtcggcctgg tcgtcaacga gtatggagac attcaagggc tggtgacggt tgaagatatt
    18181 ctggaagaga ttgtcggcga tttcactacg tcgatgtcgc caacacttgc cgaagaggtc
    18241 acgccgcaaa acgacggttc ggtgattatc gatggcaccg ccaacgtgcg ggaaatcaac
    18301 aaagccttta actggcatct accggaagat gatgcccgca cggttaatgg cgtcattctt
    18361 gaggcactgg aagagatccc tgtcgcaggc acccgcgtgc gtattggcga gtacgatata
    18421 gatattctcg acgtacagga caatatgatt aagcaggtaa aagtttttcc tgtgaaaccg
    18481 ctgcgcgaga gtgtggcgga gtaacgaaaa cggccctgca ttcgcatgca gggccgtgaa
    18541 ttattacgaa aacagaaatt acgcttttgc tttcgctaca gttaccatcg ccgcacgaat
    18601 cgtacgacca ttcagcgtat aacccttctg cataatgccc agtacgttac ctggcgcaac
    18661 gtcatcagat tccaccattg cgatagcctg atgcacattc gggtccagcg gcacgttagt
    18721 ttcggcgatc acttcaacac caaacttacg cacaacatcc agcatcgact tcagcgtcag
    18781 ctcaatgcct tcaaccatcg cagacatatc cgggttagct ttatcagcca cttccagcgc
    18841 acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca gcgcgaattt
    18901 gtgggctttt tcaatatcca gttcagtacg acgacgcagg ttttccattt cggcttttac
    18961 acgcaaaatg ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt cgagattcgc
    19021 aattttttca tcgcgcggat ccacctgctc agcagaagct tctggctcaa ctgcctcaat
    19081 ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg ttttctgttc
    19141 tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac ttcgcttatt
    19201 atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat cttcgcaaca
    19261 aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga cacccacggc
    19321 accctactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca aaaggttacg
    19381 aggtcatcgt tgagcaacaa atcgctcacg aattgcaact gaagaatgtg aaaactggca
    19441 cgctcgcgga gattgggcaa ctggctgatc tcgcggtagt cgttggtggc gacggtaata
    19501 tgctgggcgc ggcgcgcacg ctcgcccgtt acgatattaa agttattgga atcaaccgtg
    19561 gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacaa ttagccgatg
    19621 tgctggaagg tcactacatc agcgagaaac gttttttgct ggaagcgcaa gtctgtcagc
    19681 aagattgcca gaaacgcatc agcaccgcga taaatgaagt ggtacttcat ccaggcaaag
    19741 tggcgcatat gatagagttc gaagtgtata tcgacgagat ctttgcgttt tctcagcgat
    19801 ctgatgggct aattatttcg acgccaacag gctccaccgc ctattccctc tctgcgggcg
    19861 gtcctattct gaccccctct ctggatgcga ttaccctggt gcccatgttc ccgcatacgt
    19921 tgtcagcacg accactggtc ataaacagca gcagcacgat ccgtctgcgt ttttcgcatc
    19981 gccgtaacga cctggaaatc agttgcgaca gccagatagc actgccgatt caggaaggtg
    20041 aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg aaagattaca
    20101 gttatttcaa tatattaagc accaagctcg gctggtcaaa aaaattattc taattttacg
    20161 ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag taatggtttt
    20221 tcatacagga aaacgactat gttggcacaa ctgaccatca gcaactttgc tatcgttcgt
    20281 gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgagac cggcgcgggt
    20341 aaatctattg caatagatgc cctcggtctt tgtctcggtg gtcgcgctga agccgacatg
    20401 gtgcgtaccg gcgctgctcg cgctgacctg tgcgcccgtt tttctctgaa agatacgcca
    20461 gcggccctgc gctggctgga agaaaaccag cttgaagacg ggcatgaatg tttgcttcgt
    20521 cgcgtgatca gcagcgatgg tcgctcccgt ggtttcatca acggtacagc tgttcctctg
    20581 tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca cgctcatcag
    20641 ttactcacca aacccgagca ccaaaagttc ctgcttgatg gctatgccaa tgaaacctct
    20701 ctactccagg aaatgaccgc acgttatcag ttgtggcatc aaagctgccg tgacctcgcg
    20761 catcatcaac agttaagtca ggaacgcgcc gcccgtgcgg aactgctgca ataccaatta
    20821 aaagaactta acgaatttaa tccgcagccc ggagagtttg agcaaatcga cgaagagtac
    20881 aaacgtctgg cgaacagcgg tcaattgctg accaccagcc agaatgcatt ggcattaatg
    20941 gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca actggtgagc
    21001 gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga agaagctacc
    21061 atccagatcg ctgaagccag cgacgaactg cgccactact gcgatcgtct ggatctcgat
    21121 cccaaccgac tgtttgaact tgaacagcgc atctcaaaac agatttcgct ggcacgtaaa
    21181 catcacgtca gccctgaggc attaccacag tattaccagt cgctactgga agaacagcag
    21241 caactggacg atcaggccga ctcacaagaa acgctcgcgc tggcggtaac gaaacatcat
    21301 cagcaggcac tggaaacggc gcgcgcatta caccagcaac gccagcatta tgcagaagaa
    21361 cttgcacagc tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg gcagtttacg
    21421 attgatgtta aatttgacga gcatcacctg ggcgctgacg gtgccgatcg tattgagttt
    21481 cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagtcgc atccggtggt
    21541 gaattgtccc gcatcgcact ggcaatccag gtcatcacgg cgcgtaaaat ggaaaccccg
    21601 gcactgattt ttgatgaagt ggatgtaggg attagcggtc caacagcggc agttgtcggc
    21661 aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca cctgccacaa
    21721 gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg tgcgatgaca
    21781 gaaacgcata tgcaatctct ggataaaaaa gcgcggttac aagagctggc gcgcctgctt
    21841 ggtggcagtg aagtcacacg taacacactg gcgaatgcga aagaactgct tgcggcgtaa
    21901 acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc ggaaagcaaa
    21961 aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag ccacgtactg
    22021 ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg ctgcagcagc
    22081 agtactattg atgttgaccg caggctgttc cactctggag cgagtggttt accgtcctga
    22141 catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg ttggcatgac
    22201 gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat ttggtacgaa
    22261 tacctggttc tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc agcaaacgct
    22321 gacgctaacc tttaacagta gcggtgtgtt gaccaatatt gataacaaac ctgcgctgag
    22381 tggtaactaa taataaagtt gctctcaaag acgttaaaaa aggtgctcaa tgagcacctt
    22441 ttttctgtct gttatttatt cgctgatttt tctgctcgtt gcctgcgaag ctctttcgga
    22501 tcggcaatga gagggcgata aatctccacc cgatcgccat catgcacact atcgcttagt
    22561 tttgccggac ggctgtaaat gccgactttg tttttagtta aatcgatatc ggtacgcaac
    22621 tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg cagcgtcacg
    22681 cgctgcaggt actgcttctc aggtagcgca taagccacct caacggcaat tttacctggc
    22741 actgtaaacc tctttcgcac gaaccgtaaa agcctggacc atattagacg ccagctcttt
    22801 aaacacgcga ccaaaagcga gttcaatcaa cttattggta aactcaaagt cgagatgaaa
    22861 ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc catccaccaa tcaatttctt
    22921 gaacggccca tccaccagat tcataagaat actttggtta ctggtcaact ggttgcgggt
    22981 agtaaacgtt ttgctgatcc cagccttaga gacatctacc gccgcagtca tctgcccagg
    23041 agtggactcc agaatccgac ttccggtaca acccggcaaa aactgaggat aagactgaac
    23101 gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg tccggctaat
    23161 ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt atcctgctgt
    23221 aaaaaaaacg ctatcccggc gctgggtaac atcgggttca tgctaagata gagccttgtc
    23281 ccccgcagga ttgatatggg gtgttttcga tttcagatta ccgatgattc acgacgctta
    23341 tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac aagcgcgccc
    23401 gtcacgaata ctttatcgaa gaagagttcg aagcgggact tgccctgcaa ggctgggagg
    23461 ttaaatccct gcgcgcagga aaagccaata tcagcgacag ctacgtcctt ctgcgtgacg
    23521 gagaggcatt tctgtttggc gctaacatca cgccaatggc cgtggcctcc acgcatgtgg
    23581 tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca gcgcgaactg gactcattgt
    23641 acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac tggaaaaatg
    23701 cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaagaa acagcacgat aaacgttcag
    23761 atatcaaaga acgcgaatgg caggtggata aagcgcgtat catgaaaaac gctcaccgtt
    23821 aaacctgcac tccaattatt gaccagttcc tcaccgcgcc tccctctccg gcggcgcgaa
    23881 tgaacatctt attggctatc acatccgacg aaaatgttgc catcccattg ctaaatcgaa
    23941 taaaaatcag gctacatggt cgctaaatct ttaacgataa cgccattgag gctggtcatg
    24001 gcgctcataa atctggtata cttaccttta cacattgggg ctgattctgg attcgacggg
    24061 atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag ccgcaaaaaa
    24121 tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag agccctctct
    24181 ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag agatcgcgtg
    24241 gaggccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt agtggcgtgt
    24301 ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc gaggacgtag
    24361 gaatttcgga cgcgggttca actcccgcca gctccaccaa atgatgatcc ggatacgtcc
    24421 ggtgaagtac agaaagcccg cacggcacaa gccctgcggg cttttttgtg tctgtcgttg
    24481 tccgagaaca tccggctaaa tccagagaaa attggtacac gtttaggtac acgctatact
    24541 gtggtccatt aaacgtgtac caattatgga agggatccag acatggcgcg cattacacgc
    24601 cccctcacta acaacgaaat cctcaaagcg aaaccccgcg aaaaagactt caccctgcat
    24661 gatggtgatg gcctgttctt actcgtcaaa acctccggta aaaaactctg acgcttccgt
    24721 tatcaacgac cgggaagcag cagccgcaca aatttgagtc tcggctcata ccctgccctt
    24781 acactcgcag cagctcgtca gatacgcgac cagcacttaa ccacgctcgc gcaaggcatg
    24841 gatccacaac agcaacagga gcaagcgtca gcacaacgcc agattgagtt agacagcatt
    24901 ttctcaactg tggccgctaa ctggttccag attaaaagca gaagtgtcac agaggattat
    24961 gcaaaggata tttggcgttc tttggataaa gacgtgttcc cggcaatcgg tgcgatgccc
    25021 gttcaggata taaaggccag aacgattgtt gaagcattgg agccaatcaa agcacgtggc
    25081 gcattagaga cggttcgtcg cctagttcag cgtattaacg aaataatgat ttatgcggtt
    25141 aataccggtc tgatagatgc caacccggct tcgggtgtag gaatggcttt tgagaaaccc
    25201 aaaaagcaaa atatgcccac gctacggcca gaagaattac ctaagcttat gcgttccctg
    25261 gtcatgtcga atctgtctgt tccgactcgc tgtcttattg aatggcagct tctgacccta
    25321 gtgcgccctt ctgaggcttc tggtgctcgg tgggtagaga tcgatctcaa tgccaaactc
    25381 tggacgattc ccgccgaacg aatgaaggct aagcgtgaac acattgttcc tttatcacca
    25441 caagcattag aaattctgga tgtgatgaaa cctattagtg cacatcgaga acatgttttt
    25501 cccagcagaa ataatccaaa acaacctatg aatagccaaa ctgccaatgc tgcacttaag
    25561 cgaataggtt atggtgggaa attggttgca catggtttac gttcgattgc aagtacggca
    25621 atgaatgaag ccggttttaa tgctgatgta attgaggctg ctcttgctca ttcagataga
    25681 aatgaagtac gaaaagccta caatcgctca acatatttac acgcaagaat tgaattgatg
    25741 aattggtggg gacgacatat tcatccccac cagtaaactc actctggagt tcctaaggca
    25801 gtaataaaac cagcgtcaag gaactccaca ttatcggata gactatctat caatgctctt
    25861 ctccaatgat taagcccata acgtctggta tagtcatccc tagttctttt tagaaataat
    25921 cttaatcctt cttggcctgc agaattatat gtttcttcaa gtgaagtata gagcccccct
    25981 aaaaaatcat tcgcttccat ctttaacctt tcatttaggt taacaactct attttgaaag
    26041 cataatgaat catatacctc tcgagtcata ccctcgatca tattgatatt aaaatcaatt
    26101 actaaagcac catctatcat ttcagtattg gcaacgaaaa atacaccatc gggctcatca
    26161 aaataataca catgaataaa tccaactcca ttttctgcta caccagctct ttttttatta
    26221 ttgcacttat gacaggtcgg tatcaggttt cttgaatata ctgcgagaca tttatatatt
    26281 gattcgggaa gataatgatc taggtcggtt gatgattcga tcccacataa tgggcatttt
    26341 ttagaactta ataaaatcct tgagcgaagg gttgaaagcc gtccatttat ttgcacttct
    26401 gaataagctg tttttatagc attttgggtt tcatcactta tttcctctgc taaaaattca
    26461 gcacagggag ttgctttacg atcgtgatat aaagtataaa tagtttcaat tttatccttt
    26521 tcatcttgtg ttaaagcata cttaggattt ccatttttat acgttaaagc ggttaataac
    26581 tcgctcattc tatccgcatc atctggtaaa ggtagtgtcc acatatgtat tatcctttta
    26641 ccgctaattt agatattaca taagaccttg cttgtgtact aagacctgta tcaaaacatt
    26701 tttcaatgtc atctaatgga ttattattat ttttatattc tattattagc ttgtctaatt
    26761 catcatgata atcagttatt tgagtcgtta atgaaaatac tgatgatgtt attgctccaa
    26821 tattctcacc aaaagtttca atttcaggtc gttgaaaatc agtaaactcc ccatttctag
    26881 ttataacatt aacattttta cttaatgttt cttgtactac cacaggtgaa tgagtagcaa
    26941 caatagcaaa agcatcgact tcgttcaaaa caattctcag agcgctcatt aaaaccgcaa
    27001 gcaatggcgg gtgcaaatga gtttcaggct catcaaataa aacaaggcat cttttttcaa
    27061 tatacaacaa aatatgcgcc atcgaatgca aaacaaattt atgccctgta ctcaacctat
    27121 gaaaatattc tttagattta agaataaaat catctttaaa gatttcatca ataatttcag
    27181 ataatgaaac ctcttctctc aacttcatca tcactttatt aaacaaattc cacttatcac
    27241 tatctttatg tatttttatc aagacaccat aaaactcttc actaagtact tcaagaggtt
    27301 ttaatatggt tctctcttgc ctatctaatg taataaactc tatatccata ttgttttcat
    27361 ctacattagc caatacataa tccaattcag caccaacatc cctgactccg caatatatat
    27421 acctaccttt cccatcaagt atgtcttgcc ttagctgttt cttttcttta aaagtacacc
    27481 cgggtatttg aaaactatca aaagcactgt atgaaaggtt gattattttc ggaaaaccta
    27541 atccctctgg aagaatttca ccaattttgg ctatctgttc tttttttctg tcctgagttg
    27601 aagaataagc aaccctagaa agtcttgcca atgttgtact cttcccacaa ccgttccggc
    27661 ctacaattac ggacattcta catggtagtt tttcactttc aaaatatcca tattgtattg
    27721 aatcgaaatc aaacgaaaga gtattaatca ttcctgaata cataaatgaa aacttcatgt
    27781 ctatactagg tatagccgag aactgatttt ttattataaa ctttggaaga ataaaaatat
    27841 catcatcatt atttaagtct ctcatgatag aacgttccca agctctttca tttttgaaac
    27901 cctcagaaac ataatcaaac atattaatgt cgcgtaatga tgtcaatatc ccctcacaaa
    27961 tttcactccc caattcagag agcctcccat aataatcaag agattgtccc acagaacaaa
    28021 actcgtcact caaatatgaa aactcgcctt cttcaaccat gtagtaatca gcttcagact
    28081 gatctttttt tataatttta acaaccccag catactctat ttcatcaaga ccagtaagtt
    28141 tcgttgtaat gtataaatga tacttagtga taaaactaaa atcattccaa
//
LOCUS       DAZGCQ010000063        26973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000063 DAZGCQ010000000
VERSION     DAZGCQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.063"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            247..2898
                     /gene="ppc"
                     /locus_tag="ACHK07_002994"
     CDS             247..2898
                     /gene="ppc"
                     /locus_tag="ACHK07_002994"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO8103294.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            3081..4814
                     /gene="eptC"
                     /locus_tag="ACHK07_002995"
     CDS             3081..4814
                     /gene="eptC"
                     /locus_tag="ACHK07_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO8103295.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            5029..5880
                     /gene="yijO"
                     /locus_tag="ACHK07_002996"
     CDS             5029..5880
                     /gene="yijO"
                     /locus_tag="ACHK07_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8103296.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTAKLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(5867..6208)
                     /gene="frwD"
                     /locus_tag="ACHK07_002997"
     CDS             complement(5867..6208)
                     /gene="frwD"
                     /locus_tag="ACHK07_002997"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8103297.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(6210..7088)
                     /gene="pflC"
                     /locus_tag="ACHK07_002998"
     CDS             complement(6210..7088)
                     /gene="pflC"
                     /locus_tag="ACHK07_002998"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO8103298.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(7054..9351)
                     /gene="pflD"
                     /locus_tag="ACHK07_002999"
     CDS             complement(7054..9351)
                     /gene="pflD"
                     /locus_tag="ACHK07_002999"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8103299.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAAGCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(9402..9722)
                     /gene="frwB"
                     /locus_tag="ACHK07_003000"
     CDS             complement(9402..9722)
                     /gene="frwB"
                     /locus_tag="ACHK07_003000"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8103300.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(9737..10816)
                     /gene="frwC"
                     /locus_tag="ACHK07_003001"
     CDS             complement(9737..10816)
                     /gene="frwC"
                     /locus_tag="ACHK07_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO8103301.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAICVNVL
                     KSLARKKGSSTDEKEDDLDLDFEIN"
     gene            11125..13626
                     /gene="ptsP"
                     /locus_tag="ACHK07_003002"
     CDS             11125..13626
                     /gene="ptsP"
                     /locus_tag="ACHK07_003002"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8103302.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13638..14300
                     /gene="fsa"
                     /locus_tag="ACHK07_003003"
     CDS             13638..14300
                     /gene="fsa"
                     /locus_tag="ACHK07_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8103303.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDKGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            14311..15414
                     /gene="gldA"
                     /locus_tag="ACHK07_003004"
     CDS             14311..15414
                     /gene="gldA"
                     /locus_tag="ACHK07_003004"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO8103304.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            15688..16305
                     /gene="yijF"
                     /locus_tag="ACHK07_003005"
     CDS             15688..16305
                     /gene="yijF"
                     /locus_tag="ACHK07_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO8103305.1"
                     /translation="MKASLVLVSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIEDGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(16332..17237)
                     /gene="yijE"
                     /locus_tag="ACHK07_003006"
     CDS             complement(16332..17237)
                     /gene="yijE"
                     /locus_tag="ACHK07_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO8103306.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(17331..19511)
                     /gene="katG"
                     /locus_tag="ACHK07_003007"
     CDS             complement(17331..19511)
                     /gene="katG"
                     /locus_tag="ACHK07_003007"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO8103307.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(19840..20730)
                     /gene="metF"
                     /locus_tag="ACHK07_003008"
     CDS             complement(19840..20730)
                     /gene="metF"
                     /locus_tag="ACHK07_003008"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO8103308.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            20806..21051
                     /locus_tag="ACHK07_003009"
     CDS             20806..21051
                     /locus_tag="ACHK07_003009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103309.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLTASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(21079..23511)
                     /gene="metL"
                     /locus_tag="ACHK07_003010"
     CDS             complement(21079..23511)
                     /gene="metL"
                     /locus_tag="ACHK07_003010"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO8103310.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(23514..24674)
                     /gene="metB"
                     /locus_tag="ACHK07_003011"
     CDS             complement(23514..24674)
                     /gene="metB"
                     /locus_tag="ACHK07_003011"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO8103311.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            24951..25268
                     /gene="metJ"
                     /locus_tag="ACHK07_003012"
     CDS             24951..25268
                     /gene="metJ"
                     /locus_tag="ACHK07_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO8103312.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            25328..25936
                     /gene="yiiX"
                     /locus_tag="ACHK07_003013"
     CDS             25328..25936
                     /gene="yiiX"
                     /locus_tag="ACHK07_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO8103313.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            26081..26756
                     /locus_tag="ACHK07_003014"
                     /pseudo
     CDS             26081..26756
                     /locus_tag="ACHK07_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
     gene            complement(26843..>26973)
                     /locus_tag="ACHK07_003015"
     CDS             complement(26843..>26973)
                     /locus_tag="ACHK07_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO8103314.1"
                     /translation="VFFYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         6544 a         7481 c         6872 g         6076 t
ORIGIN      
        1 ctttggtgtt acttgaggcg attttttaac attcccataa gttacgctta tttaaagcgt
       61 cgtgaattta atgacgtaaa ttcctgctat ttattcgttt gctgaagcga tttcgcagca
      121 tttgacgtca ccgcttttac gtggctttat aaaagacgac gaaaagcaaa gcccgagcat
      181 attcgcgcca atgcgacgtg aaggatacag ggctatcaaa cgataagatg gggtgtctgg
      241 ggtaatatga acgaacaata ttccgcattg cgtagtaatg tcagtatgct cggcaaagtg
      301 ctgggagaaa ccatcaagga tgcgttggga gaacacattc ttgaacgcgt agaaactatc
      361 cgtaagttgt ccaaatcttc acgcgctggc aatgatgcta accgccagga gttgctcacc
      421 accttacaaa atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt tagtcagttc
      481 ctgaacctgg ccaacaccgc cgagcaatac cacagcattt cgccgaaagg cgaagctgcc
      541 agcaacccgg aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc ggaactgagc
      601 gaagacacca tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct cacggctcac
      661 ccaaccgaaa ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa cgcctgttta
      721 aaacagctcg ataacaaaga tatcgctgac tacgaacaca accagctgat gcgccgcctg
      781 cgccagttga tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg tccaagcccg
      841 gtagatgaag ccaaatgggg ctttgccgta gtagaaaaca gcctgtggca aggcgtaccg
      901 aattacctgc gcgaactgaa cgaacaactg gaagagaacc tcggctacaa actgcccgtc
      961 gaatttgttc cggtacgttt tacctcgtgg atgggcggtg accgcgacgg caacccaaac
     1021 gtcactgccg atatcacccg ccacgtcctg ctgctcagcc gttggaaagc caccgatttg
     1081 ttcctgaaag atattcaggt gctggtttct gaactgtcga tggttgaagc gacccctgaa
     1141 ctgctggcgc tggttggcga agaaggtgcc gcagaaccgt atcgctatct gatgaaaaac
     1201 ctgcgttctc gcctgatggc gacacaggca tggctggaag cgcgcctgaa aggcgaagaa
     1261 ctgccaaaac cagaaggcct gctgacacaa aacgaagaac tgtgggaacc gctctacgct
     1321 tgctaccagt cacttcaggc gtgtggcatg ggtattatcg ccaacggcga tctgctcgac
     1381 accctgcgcc gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat ccgtcaggag
     1441 agcacccgcc ataccgaagc gctgggcgag ctgacccgct acctcggtat cggcgactac
     1501 gaaagctggt cagaggccga caaacaggcg ttcctgatcc gcgaactgaa ctccaaacgt
     1561 ccgcttctgc cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct cgatacctgc
     1621 caggtgattg ccgaagcgcc gcaaggctcc attgccgcct acgtgatctc gatggcgaaa
     1681 acgccgtccg acgtgctggc tgtccacctg ctgctgaaag aagcgggtat cgggtttgca
     1741 atgccggttg ctccgctgtt tgaaaccctc gatgacctga acaacgccaa cgatgtcatg
     1801 acccagctgc tgaatatcga ctggtatcgc ggcctgattc agggcaaaca gatggtgatg
     1861 attggctatt ccgactcagc aaaagatgcg ggcgtgatgg cagcttcctg ggcgcaatat
     1921 caggcacagg atgcattaat caaaacctgc gaaaaagcgg gtattgagct gacgttgttc
     1981 cacggtcgcg gcggttccat tggtcgcggc ggcgcacctg ctcatgcggc gctgctgtca
     2041 caaccgccag gaagcctgaa aggtggcctg cgcgtgactg aacagggcga gatgatccgc
     2101 tttaaatatg gtctgccaga aatcaccgtc agcagcctgt cgctttacac cggggcgatt
     2161 ctggaagcca acctgctgcc accacctgag ccgaaagaga gctggcgtcg cattatggat
     2221 gaactatcag tcatctcctg cgatctctac cgtggctacg tacgtgaaaa caaagatttt
     2281 gtgccttact tccgctccgc tacaccggaa caagaactgg gcaaactgcc gttgggttca
     2341 cgtccggcga aacgtcgccc gaccggcggc gtcgagtcgc tgcgcgccat tccgtggatt
     2401 tttgcctgga cgcaaaaccg tctgatgctc cccgcctggc tgggtgcagg tacagcgctg
     2461 caaaaagtgg tcgaagatgg caaacagagc gagctggagg ctatgtgccg cgattggcca
     2521 ttcttctcga cgcgtctcgg catgctggaa atggtattcg ccaaagcaga tctgtggctg
     2581 gcggaatact atgaccaacg cctggtagac aaagcactgt ggccgttagg taaagagtta
     2641 cgcaatctgc aagaagaaga catcaaagtg gtgctggcga ttgcgaacga ttcccatctg
     2701 atggccgatc tgccgtggat tgcagagtct attcagctac ggaatattta caccgacccg
     2761 ctgaacgtat tgcaagccga gttgctgcac cgctcccgcc aggcagaaaa agaaggccag
     2821 gaaccggatc ctcgcgtcga gcaggcgtta atggtcacta ttgccgggat tgcggcaggt
     2881 atgcgtaata ccggctaatc ttcctctcct gcaaaccctc gtgcttttgc gcgagggttt
     2941 tctgaaatac ttctgttcta acaccctcgt tttcaatata tttctgtctg tattttattc
     3001 aaattctgga tatcccttca gatatcctta aggaattgtc ggtacattcg gcgatatttt
     3061 tttcaagaca ggttcttact atgcattcca cagaagtcca ggctaaacct ctttttagct
     3121 ggaaagccct gggttgggca ctgctctact tttggttttt ctctactctg ctacaggcca
     3181 ttatttacat cagtggttat agtggcacca acggcattcg cgactcgctg ttattcagct
     3241 cgctgtggtt aattccggta ttcctctttc cgaagcggat caaaattatt gccgcagtga
     3301 tcggcgtggt gctatgggcg gcctctctgg cggcgctgtg ctactacgtc atctacgggc
     3361 aggagttctc gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac gaagccagcg
     3421 agtatttaag ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc tatacggcag
     3481 tggcagttct gctgtggaca cgtctgcgcc cggtctatat tccaaagccg tggcgttata
     3541 ttgtctcttt tgccctgctt tatggcttga ttctgcatcc gatcgccatg aatacgttta
     3601 tcaaaaacaa gccgtttgag aaaacgttgg ataacctggc ctcgcgtatg gagcctgccg
     3661 caccgtggca attcctgacc ggctattatc agtatcgtca gcaactaaac tcgctaacaa
     3721 agctactgaa tgaaaataat gccttgccgc cgctggctaa tttcaaagat gaatcgggta
     3781 acgaaccgcg caccttagtg ctggtgattg gcgagtcaac ccagcgtgga cgcatgagtc
     3841 tgtacggtta tccgcgtgaa accacgccgg agctggatgc gctgcataaa accgatccga
     3901 atctaaccgt gtttaataac gtagttacgt ctcgtccgta caccattgaa atcctgcaac
     3961 aggcgctgac ctttgccaat gaaaagaacc ctgatctgta tctgacgcag ccgtcgctga
     4021 tgaacatgat gaaacaggcg ggttataaaa ccttctggat caccaaccag cagacgatga
     4081 ccgcccgcaa taccatgctg acggtatttt cgcgccagac cgacaagcag tactacatga
     4141 accagcaacg tacgcagagt gcgcgtgaat acgacactaa cgtgctgaag ccgttccagg
     4201 aagtgctgaa ggaccctgcg ccgaagaaac tgatcattgt tcatctgctg ggtacgcata
     4261 tcaaatacaa ataccgctac ccggaaaatc agggcaagtt tgatggcaat accgatcatg
     4321 ttccaccggg attaagcgca gaagaactgg agtcatataa cgattatgac aacgctaacc
     4381 tgtataacga tcatgtggtt gccagcctga ttaaagactt taaagcggca gacccgaacg
     4441 gattcctggt ttacttctct gaccacggtg aagaggttta cgacacaccg cctcataaaa
     4501 cccaggggcg taatgaggac aacccgacgc gtcatatgta caccattccg ttcctgctgt
     4561 ggacgtcaga aaaatggcaa gcgactcatc cgcgtgattt ctcgcaggat gttgatcgta
     4621 aatacagcct tgcggaactg atccacacct ggtcagattt agcaggctta tcttacgacg
     4681 gttacgatcc aacccgttca gtggtgaatc cgcagttcaa agaaactacc cgctggattg
     4741 gtaacccgta caagaaaaac gcgctgatcg attacgacac gctgccatat ggcgaccagg
     4801 taggtaatca gtaattatca gccaggcgct atttgccgga tgcgacgctt gacgcgtctt
     4861 atccggccta cagatcgcgg cggtagactg gataaggcgt ttacgccaca tccggcaata
     4921 caactgccgc aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt
     4981 taataatgcc agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac
     5041 gtcagctatc tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt
     5101 gccagcagta acggcccggt gcccgatctt gcgtatcagg tcgattttcc acggctggaa
     5161 attgtgctgg aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat
     5221 gtgctgtacg ttcctgctgg tggctggaat tttccacaat ggcaagcccc cgccactacc
     5281 tttagcgtgc tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa
     5341 caatatcaaa atctggcgaa gcaacacgtc gcccgacgcg gcccacgcat aggttctttt
     5401 ctgctacaaa cgctcaatga aatgcaaatg cagccgcagg agcagcaaac ggcaaagctt
     5461 atcgtcgcca gcctgcttag ccactgccgc gatttgctcg gcagccaaat ccagaccgcc
     5521 tcacgcagcc aggcactgtt cgaagctatt cgtgattata tcgacgaacg ctatgcctcc
     5581 gcgcttaccc gcgaatctgt cgcacaggca ttctatattt caccaaacta tctctcgcac
     5641 ctgtttcaaa aaacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag
     5701 cacgctaaga cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc
     5761 ggctttgtcg acagcaacta cttctgccgt ctgtttcgta aaaacaccga acgctcgccg
     5821 tcggaatatc gtcggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa
     5881 gatgggtttg ctgcggcgca gaaagcactt tgcgcaccgc gctcattacc cgctgcggct
     5941 cacgcaggaa tgcgtagatg ctgcattgca catagcggca atgttcaaat cgctcggcac
     6001 cggcaagctc gatatccgta atcaacagag caacatccgc ccgacggata tcctcgtccg
     6061 ctaaacgatt ctccgttccc agcgcgccct gagtttcaat gctgatgccc cacttctcta
     6121 actggcacaa cttttccagc cgttccgccg ccatataagt atgcgccacg ccgcttacgc
     6181 aggcggttac tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc
     6241 cgttctgcca tttcgcgcat cgttgccaca tcagctgacg acggcgcagg cacctctttc
     6301 atcgaccatg ttttccccag caggcggtat ttcggttcgc cgtactgatg aaacggtaac
     6361 agatggatct gcctgatatt cagcgggatc agcacgtcca gcgcctgctg catattctcc
     6421 cggctgagcg tgaaaccagg gatcagcggt aaacgcggga tcacgttgac gccctcactc
     6481 accagcaaac gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc
     6541 tgagtcgcgt ccataatttt taaatcgaac aacacttcat cgcacaattt cgccagtggt
     6601 aacagcttgc tggctggtgc gtctccggca gtttcaatgg cgcatgacac accccacagc
     6661 cgcagtcgct gtaaaaaacg ggtagcaaac tccgcctgca ttaacacttc gccgccagaa
     6721 agcgtgacgc cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc
     6781 agcgcgtcaa ggctgatatc gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc
     6841 gcatcacgca aacatttcgc acagtgcaga catttcgcct ctctgcgtac agtctggatt
     6901 ttgccggaga tcgactccgg attagcacac cacgggcaaa gatgtggaca gcctttaaaa
     6961 aagaccaccg tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg
     7021 cgcgccacat cattgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat
     7081 gatgtcatcc tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg
     7141 caccaccagc ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt
     7201 caccacgtta aactgaatat gttgtaactt aagctgggta aacgcccgta agaagtcggc
     7261 cagtttgcgt aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa
     7321 cgtaccgtta gacagcagcg tattatcgag cttactgact gacttcagta ccgctgttgg
     7381 cccttgtgcg tcctggccca gcatgggtga caagccgccg tctgccagct gttctccggc
     7441 aaaacgaccg tctggcgtcg cgccaaccac cgatcccaac gggacgtgag cagaaacggt
     7501 atacgatccc ggcgtgaagt aaccgccgcg cgggttctgg tatttttcca cttctttgca
     7561 gtagtggcgc aacagctcgg cgctaatgtt atccacctcg tcgatatcgt taccgtattt
     7621 ctcaaagcgg ttaattaagc gagcgcggac tttttcgcct tctggcgttg caaagttggc
     7681 ttttaatacc gacagcaatt cgtcaaaact taaacgctgt tgatcaaaaa ccatcccttt
     7741 caacgcatgg agagaatcgc tcaggttggc gataccaatt ccctgtacgc cggagaagtt
     7801 ataacgcgcg ccgccatcgg taatatcgcg gcctttttcc agacaatcgc tgataaacga
     7861 tgagagcagc ggtacaggtg cccagtcgcg atggccgata tcacaaatat tgctgccctc
     7921 aaccatcagg gtgatgtagt ggctgatctt ggcgcggatc tgctccagta aaccttcata
     7981 agtcagcgca gcattgcctt cattttcatg caggcagatt tccatcactt tcagcagatt
     8041 aaacatcgcg atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc
     8101 cactacggaa tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggcac
     8161 caccacttca tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttctgcggt
     8221 tttcatcagg aacggcgtgt cgatcaaggc gttagtgcgc acccctaggt tcggttgcgg
     8281 taattgtacg ctttgatagg catcgaggca gaggaacgaa agcacgttca ccgcgctacg
     8341 tccgttctcg gttaacccgc cgagcagtgc ggtatagcca gtcgggaaac ctgcgaaata
     8401 acgcgcgctg ctggtggagc gcaacagcac aatgtcgttg catttcaccc ataaagattc
     8461 gagcagttct ttcaggaacg ccggatcttc gccctgggtt aaagatgcct gatagaacgg
     8521 caacatatac tggtcgaagc gccctaaaga cagcgaactg gcgttggatt cgtattgcag
     8581 aatgatgttc atgtaccaga ataactggca cgcctgccag aacgtctgcg gtttatgttc
     8641 ggcgttatgg cgggagatct ccgcaatagt cagcagttct tcgcgacgct gggcatcagt
     8701 gcagcctgcc gccatcgttt ccgccagttc ggcgtaacgc aaaatgtgtt tctgcgaggc
     8761 ttcgagcagt aacagcgctg cctgataaaa gtgattctcc ggctgttgct gacaatgttg
     8821 ctgcatctgc gccaccagct cccccagccc gtgattcagc aggcgcgggt aatcaataat
     8881 aatgtgcccc tggcctttat ccgtctggtt gatgctgaaa atctgcgtgc tggtcgcggc
     8941 ttttacttca tccgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta
     9001 cgggaacaac tcttcgcggt agatacgttt atcttcttcg ctgatagcaa agcggtcctg
     9061 cggacgcgtc gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga
     9121 cataatcccg gcgcgtggtt tcaccgtgcg gttaccggca atcagttctt catcacgaat
     9181 cgaaatttca acatgttcaa ggatatacgc tgttgctttc gcccggcgca atatcaccgg
     9241 ttcgccttcg gtttgccgat ggctggcggt ataaagcagc gcacgctcca gcgagatttc
     9301 acgggtattg gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc
     9361 ctgtgggaaa gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt ttgcgccagg
     9421 tgagcttcaa ttttgctcat aatggcgtca gcgcgtttta ccgcgtcgct gatgttgacg
     9481 cgcacaatgg tcttgcccgc aaaacgctct tcaaacttga tgccgatatc tttggtcaga
     9541 atcaccatat cggcgctcgc cacatcttcc gcagtcagtt cgttttccag accaatcgag
     9601 ccctgggttt ctactttcac ttcccagcct ttcgctttcg cggcactttc cagcgcctct
     9661 gccgccatat aggtatgggc aacaccagat ggacatgcgg ttactgcaat aatttttgtc
     9721 atggctcgtt cctcaattaa ttaatttcaa aatccagatc caggtcgtct tccttctcat
     9781 cagtcgaaga ccctttttta cgcgccagac ttttcagcac gttcacacaa atagccgtca
     9841 ccactgcccc gacagccact gctgcgatat aacccagctt gccttcaacc accggcagta
     9901 caatcagacc accccagcct gcatagcact gcgcaccgac cagcgccgca gttactgcac
     9961 cacaaactga accgaccatg atggaaggaa taacacgcag cggatcggca gcggcgaaag
    10021 gaatcgcccc ttcagtaacc ccaacgcacc ccatcaccag cgccgcttta ccggtttcgc
    10081 gctcttctgc ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg
    10141 ggatacaaat acccacggca gcaatggcca ccacggtata aacaccctga gcaacgcaaa
    10201 tcagcatgaa ggcataggcc actttgttaa ccggaccgcc catatcgaac gccagcatca
    10261 gacccatgat caccgccagc ataacaatgc tgccctgctg catcccctga agccactgag
    10321 tcaggctgtt ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg atgcctgcgg
    10381 taatcagtgt gccgatgata ggaatgatga agataggcat caccgagcgc agaactttat
    10441 gcaccggaat tttcttcagg taatgcacca cgatgccgcc gataatcccg gcgatcagtg
    10501 caccaaagaa gcccgcacca aagctgttac caacccaggc accgatagcg cacggagcca
    10561 gcgcagaacg ctctgcaatg gagtaaccga tgtaagcggc gaggaaaggc accatcagcg
    10621 tcaaacctgc aacgccgata tcaaacagtt ttttcagatt cggatcggct acggcatccg
    10681 gcactgcgcc tttgccatac aacatgacgg aaaccgccag caaaataccg cccgatacca
    10741 cgaagggaat catgtgtgaa acgcccgtca ttaaatgctg acgggtattt ttcaggatct
    10801 gcaccaactc attcataagc tgctcccggg cttgcgaaag cccatgtcca tatttttgat
    10861 ggaggaaaca ataggcaatc gcaggcggag caaacagtgg ataaaaaggc catttactgg
    10921 acttttgtaa tatcagtaca aaaatgcgat ccgcctcata acttgcgata atccggaagc
    10981 atgccgcaaa acggcgtctt gtgagacgcg tcgcaaattg acggttacat tacatttgtc
    11041 cagcttttgg cagatttgtc acatggcagg ccgttgccgt gcgctcttat cctgtaaaca
    11101 gaaaaccgta ccgggagaga ggcaatggcc ctgattgtgg aatttatttg tgagctacct
    11161 aacggcgtac atgcacgtcc ggcaagccac gttgaaacgc tgtgtaatac tttttcatca
    11221 caaattgagt ggcataacct gcgcactgac cgcaagggca acgccaaaag cgcccttgcg
    11281 ctgattggca ccgacacgct ggcgggcgat aactgccagt tactgatttc cggggccgac
    11341 gaacaggaag cgcaccagcg tttaagccaa tggctgcgcg atgaattccc ccactgcgat
    11401 gcgccgctgg cggaagttaa atctgacgaa ctggaaccac tgccggtttc actgaccaat
    11461 ctgaatccgc aaattatccg cgcccgcacc gtgtgcagcg gtagcgcagg cggcattctg
    11521 acgccgatct cttctttaga tctcaatgcg ctgggtaatc ttcccgcagc caaagacgtt
    11581 gacgccgagc aatccgcact ggaaaacggc ctgacgctgg tactgaaaaa cattgagttt
    11641 cgtctgctgg atagcgacgg tgccaccagc gcgattctgg aagcgcaccg atccctggct
    11701 ggcgatactt ccctgcacga acatttactg gcaggcgtca gcgccggatt aagctgcgcc
    11761 gaagcaattg ttgccagcgc gaatcacttt tgcgaagagt tcgcccgttc cagcagcagc
    11821 tacctgcaag aacgtgccct ggacgtacgc gacgtctgct tccagttact ccagcaaatc
    11881 tacggtgagc aacgcttccc ggcaccgggc aaactgacgc agcccgccat ttgtatggct
    11941 gatgaactga cccccagcca gttcctcgaa ctggataaaa atcacctcaa aggattgtta
    12001 ctgaaaagcg gcggcaccac ctcacatacc gtaattcttg cccgttcgtt caacattccg
    12061 acactggttg gtgtagatat tgatgccctt actccgtggc agcatcaaac gatttatatc
    12121 gacggcaacg ccggggcgat tgtggttgag ccaggggaag ccgtagctcg ttattatcag
    12181 caagaagccc gcgtacagga cgcccttcgt gagcaacagc gtgtctggct gacccaacaa
    12241 gcccgtaccg ctgacggtat ccgcattgaa attgccgcta acatcgctca ctccgtggaa
    12301 gcgcaggccg cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac tgaaatgctc
    12361 tatatggatc gcaccagtgc accgggcgaa agcgagctgt acaacatttt ttgtcaggcg
    12421 ctggagtccg ccaacggacg cagcattatt gtgcgcacta tggatattgg cggtgacaaa
    12481 cccgttgatt atctgaacat tcccgcagag gcaaacccgt tccttggtta tcgcgccgtg
    12541 cgtatttatg aagagtacgc gtcgttattt accacacaac tacggtcgat tctccgtgcc
    12601 tccgctcacg gcagcctgaa aatcatgatc ccgatgatct cttcaatgga agagatctta
    12661 tgggtgaaag aaaaactggc ggaagccaaa cagcaactgc gtaacgaaca cattccgttt
    12721 gatgagaaga tccagctcgg catcatgctg gaagtgccgt cggtgatgtt catcatcgat
    12781 caatgctgcg aagagattga tttctttagt attggtagta atgacctgac gcaatatctg
    12841 ctggcggtgg atcgcgataa cgctaaggtt actcgtcact acaacagcct gaatccggca
    12901 ttcttgcggg cgctcgatta cgccgtgcag gcggtgcatc gccagggcaa atggattggt
    12961 ctgtgcggtg aactgggagc gaaaggttcc gtgctgccgt tgctggtcgg tttagggctg
    13021 gatgaactca gcatgagcgc accgtcaatt ccggcggcga aagcgcgaat ggcgcaactt
    13081 gatagccgtg agtgccgcca gttgctcaac caggcaatgg cctgccgtac ttcgctggaa
    13141 gtggaacatc tgctggcgca attccgcatg acccaacagg acgcaccact ggtcaccgcc
    13201 gagtgcatca cgctggaaag cgactggcgc agcaaagaag aagtactcaa aggcatgacc
    13261 gataacctgc tgctggcggg ccgctgccgc tatccgcgta aactggaagc cgacttgtgg
    13321 gcgcgcgagg ccgttttctc taccggtctg ggctttagtt ttgccattcc ccacagcaaa
    13381 tcagaacaca ttgagcagtc caccatcagc gtagcgcgtc tgcaagcgcc ggtgcgctgg
    13441 ggcgatgatg aagcgcaatt catcatcatg ttaaccctga acaaacacgc tgcgggcgat
    13501 caacatatgc gcattttctc gcgcctcgct cgtcgcatca tgcacgaaga attccgtaac
    13561 gcgctggtta acgccgcctc tgccgacgct atcgccagcc tgctgcaaca tgaactggaa
    13621 ctgtaaaagg aaacatcatg gaactgtatc tggacaccgc taacgtcgct gaagtcgaac
    13681 gtctggcacg cattttcccg attgccgggg tgacaactaa cccgagcatt atcgctgcca
    13741 gcaaggagtc catctgggaa gtgctgccgc gtctgcaaaa agcgattggt gataagggca
    13801 ttctgtttgc tcagaccatg agccgcgacg cgcaggggat ggtggaagaa gcgaagcgtc
    13861 tgcgcgacgc cattccgggc attgtggtga aaatcccggt gacttccgaa ggtctggccg
    13921 ccattaagat gctgaaaaaa gagggtatta ctacgcttgg caccgcagtg tacagtgctg
    13981 cacaagggtt actggccgcg ctggcagggg caaaatacgt tgctccgtat gttaaccgcg
    14041 tagatgccca gggcggagac ggcattcgta cggttcagga gctgcaagcg ctgttagaaa
    14101 tgcacgcgcc agaaagcatg gtgctggcag ccagctttaa aacgccgcgt caggcgctgg
    14161 actgtttact ggcaggatgt gaatccatca ccctgccctt agatgtagcg caacaaatgc
    14221 tcaacacccc tgcggtagag tcagctatag agaagttcga acacgactgg aatgccgcat
    14281 ttggcactac tcatctctaa aggagcaatt atggaccgca ttattcaatc accgggtaaa
    14341 tacatccagg gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc gctggcagaa
    14401 cgctggttag tggtaggtga caaatttgtt ttaggttttg ctcaatccac tgtcgagaaa
    14461 agctttaaag atgctggact ggtagtagaa attgcgccgt ttggcggtga atgttcgcaa
    14521 aatgagatcg accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc aattctcggt
    14581 atcggtggcg gaaaaactct cgatactgcc aaagcactgg cacatttcat gggtgttccg
    14641 gtagcgatcg caccgactat cgcctctacc gacgcaccgt gcagcgcatt gtctgttatc
    14701 tacaccgatg agggtgagtt tgaccgctat ctgctgttgc caaataaccc gaatatggtc
    14761 attgtcgaca ccaaaatcgt cgctggcgca cctgcacgtc tgttagcggc gggtatcggc
    14821 gatgcgctgg caacctggtt tgaagcgcgt gcctgctctc gtagcggcgc gaccaccatg
    14881 gcgggcggca agtgcaccca ggctgcgctg gcactggctg aactgtgcta caacaccctg
    14941 ctggaagaag gcgaaaaagc gatgcttgct gccgaacagc atgtagtgac tccggcgctg
    15001 gagcgcgtga ttgaagcgaa cacctattta agcggtgtag gctttgaaag tggtggtctg
    15061 gctgcggcgc acgcagtgca taacggcctg accgctatcc cggacgcgca tcactattat
    15121 cacggtgaaa aagtggcatt tggtacgctg acgcagctgg ttctggaaaa tgcgccggtg
    15181 gaggaaatcg aaaccgtagc tgcccttagc catgcggtag gtttgccaat aactctcgct
    15241 caactggata ttaaagaaga tgtcccggcg aaaatgcgaa ttgtggcaga agcggcatgt
    15301 gcagaaggtg aaaccattca caacatgcct ggcggcgcga cgccagatca ggtttacgcc
    15361 gcactgctgg tagctgacca gtatggacaa cgtttcctgc aagagtggga ataacctact
    15421 ccaaactccc ggcttgtccg ggagtttgaa cgcaaaattg cctgatgcgc tacgcttatc
    15481 aggcctacgc aatctctgca atatattgaa tttgcgtgct ttttaggcca gataaggcgt
    15541 tcacgccgca tccggcactc tgtgccagta atctgaaact cctacgttgt tcaggagttt
    15601 tttatccgct tctggacatt ttctctacag aacaatcgtt atcccggaag gtcgaatctg
    15661 tgactcttat tttcacggac gaaacagatg aaagcttcac tggtgctggt cagcctgcta
    15721 acggcattta ccagccactc cttaaaatct cccgccgttc caccaaccgt ggtacaaatc
    15781 caggccaata ccaaccttgc catcgaagac ggtgccagac agcaaatcgg cagcacgcta
    15841 ttttacgatc cggcgtatgt gcagcttacc tatcctggcg gtgatgtccc gcaagaacgc
    15901 ggggtatgtt ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga tttgcagaaa
    15961 ctggtgcacg aagatatggc gaagaatttt gccgagtacc cgcaaaaatg gcagttaaag
    16021 cgcccggaca gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg gtttacccgc
    16081 cacgataaaa cacgccccat cagcaagaac cccagtgact atcaagcggg cgatattgtc
    16141 tcctggcgat tagataacgg gctggcacat attggggtgg tttcagacgg cttcgcccgc
    16201 gacggtacgc cgctggtgat acataacatt ggcgcaggtg cgcaggaaga agatgtgctg
    16261 tttagctggc gcatggtggg gcattatcgt tattttgtta aataagcgat cgccccacat
    16321 gaagaaaaaa ttcagatcct ttttacacta acggcttctt ttttcttacg gctcaccagc
    16381 gccagtgcca gcacaatcag cacaataccg ctaccttcaa cggcccccgg attctcaccg
    16441 agcagccacc aggaaaagag tacgccgcaa acgggaacgg ccagtgtgct taagctggca
    16501 atactggcag gcaagttttt cagcacaaac agccataagc tccacgccaa cgccgtcgcc
    16561 agaatcgcac tgtaggccag agcccagaac acagtgggct gccagtcaat ttcacgttgc
    16621 ggcaccagta aagcgaccac actcatcacc agcgccgcat agagcatctg ccaggatgtt
    16681 aacgacaata aatccacgcg cggatgacgg gcatacagac gtttagcaac aatcgcgctc
    16741 gccccccaac taacaccgga gaggattgcc agcatggcac ttttcatcga agagaaatcg
    16801 agttgccacg gctgcaacac caaaaataag ccgaaagcgg caatcagaat cgcgaaatat
    16861 tgtccacgtc gcaggcgttc accgagaaac aacgcggcga aaatcaccac ccagaacggc
    16921 atggtatagc tcaggatcgc cacctttccc gctccgccgc tgaccagcgc ccactgcgcc
    16981 aggccaacca tcccacaggt ttgtaacagc gcaatggcta aggtgtattt aaacggcgtc
    17041 gggcgcattc cgcgaccacg taataaaagg acgatgaata aaacgagagc gccgaaaatg
    17101 cagcgtaagg cggtaaagtc gaaggcaccg atgtagctgg tgacttgctt catgaaaatc
    17161 cagctataac tccagataag tgtgagcaca accaggccac tgattgccag tgggttgctc
    17221 tttcctgcgg cagacatgag agatccagtg tgtagttttc ctgtggacac tatacgccgg
    17281 taaagaaact tcagcgactg cctgaaaggc agtcgctgaa gaaggtcaga ttacagcagg
    17341 tcgaaacggt cgaggttcat cactttcacc catgccgcca cgaagtcttt aacaaacttc
    17401 tcgtgggcat cgctgctggc gtaaacttca gccaccgcac gcaggacaga gttagaacca
    17461 aataccagat ccgcacggct ggcagtgtat ttcacttcgc cggtttcgcg gtcacggcct
    17521 tcgaacagct ctttcgattc gtcggtcgct ttccactcgt aacgcatatc cagcaagttc
    17581 acgaagaagt cattgctcaa tacgccaacg cggtcagtga agacgccgtt tttgctgcca
    17641 tcgaagttgg cacccagtac acgcatcccg cccaccagcg cggtcatttc cggcgcggtc
    17701 agggtcagtt gctgggcttt atcaatcagc agtgactcgg tggtggaaac atccagacga
    17761 gcgcgatagt tacggaaacc gtcagcaatt ggctccagca gttcgaacat ctcaatgtca
    17821 gtctgatcct gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat gctcaaacca
    17881 gcggcgcttg cggctttctc aacgccaacc acacctgcca gcacgatgat atccgccagc
    17941 gaggctttac cagactcttt ctgaattttc tccagaactg gcagcgcacg aacggctgct
    18001 gcgttcacat cccagtcgcg ctgcggcatt aatgccagac gcgcaccgtt ggcaccaccg
    18061 cgtttgtcgc caccacggaa ggtagaagca gaagcccagg ccaccgatac cagctcacta
    18121 acagacagac cagaatccgc aatcgcgaat ttcagatcga taatgtcctg ctcggtcggg
    18181 ttgtagatcg gctgcggcag cggatcttgc cagatcagat cttctttcgg cacttccggc
    18241 ccgatgtagc gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca ggcacgggca
    18301 aaggcttcgt tgaacgcctg cggatcgttg aggaaacgac gagagatctt ctcgaactca
    18361 ggatcaaagc gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt cttcgacgga
    18421 tcaaacggat ccggaataat ttccggtgcg tctaccgctt cgaactggat tgcgccagcc
    18481 gggctgcggg tctgtaccca ctcatacttg aacaggttct cgaagaaata gttgctccac
    18541 tgggtcggcg tctgggtcca gactacttcc agaccagagg taatggcatc tgcgccaacg
    18601 ccgctgccgt aagtgctcgc ccaacctaaa ccttgttctt caatcggtgc agcttctgga
    18661 tcaggaccta catttgatgt cggaccagca ccgtgggttt tacccagcgt atgaccaccc
    18721 gcaatcagcg ccacggtttc ttcgtcgttc atacccatgt tgccgaaggt cgcgcggata
    18781 gctgctgccg cagaaagcgg ttcgccgctg tgatccgggc cttccgggtt aacgtaaatc
    18841 aggcccatct cggttgcacc cagcggtgct ttcgccagcg cttccggatg acggtgagtc
    18901 agccaggctt tttcatcacc ccagttaaca tccagatccg gttcccagac gtcttcacga
    18961 ccggcaccaa aaccgaaggt acggaagccg gagttttcca gcgccacgtt acccgcgagg
    19021 ataaacaggt cggcccagga gattttctga ccatatttct gtttgattgg ccacaacagg
    19081 cgacgtgctt tatcgaggct tacgttatcc ggccaggagt tcagcggtgc aaaacgttgc
    19141 tgaccacgac ccgcgccgcc gcgcccatcg attgaacggt aagtacccgc accgtgccag
    19201 gccatacgaa taaacaaacc ggcgtagctg ccccagtcgg ctggccacca cggttgagat
    19261 tctgtcagca gggctttcag atcttttttc aggccgtagt aatctaattt gctgaattct
    19321 ttgcggtagt caaagtcctc acccagcggg ttagaacgat tagaatgttg gtttaacagg
    19381 tcaacacgga gttgatttgg ccaccagtcg cgagtggttg tgcccgcccc cgcgctctgg
    19441 tcgtgaccgc cctgatggaa cgggcatttg ccagtggctg tcgtgttatg gatatcgtct
    19501 gacgtgctca tcaatgtgct cccctctaca gtgttaccgt tacgatacac agcgttagag
    19561 agaagttata attgaattaa tccacggatg cgatagttga gatcttacat attgttggtt
    19621 aaagagatgt agatcaaatt gatcgtaatt aagctttttt gattatgtcc gaattcggac
    19681 aaatgcttta taaaaaaggg ttttagcttc atatccttca agtaatgagg gagaaaacaa
    19741 ggctgataac cttattttcc cgccctcatt tcgaggcagc attttgtgct ctgtttaaaa
    19801 tttgtgatca ctgtgtaatt ttcgcaaaag ccacactatt tataaaccag gtcgaacacc
    19861 cagcgtatgg caaatcgcgt aactcatttc agcgcggtta agcgtataga agtggaaatc
    19921 tttcactcct tcacggctta aaatcttcac catatccatg gcgatattcg cgcctaccag
    19981 cttgcgggtt tcggcgtcat catccaggcc gtcgaacatt tgcgccatcc acgccggaat
    20041 acgcacgttg gtcatatcgg caaatttctt cgcctgttta aagttagata ccggcaaaat
    20101 tcccggaata atttccacat caatgcccgc cgatacacag cggtcacgaa aacgcaggta
    20161 gctttcgaca tcgaagaaga actgagtaat cgcgcggttg gctccggcat cgactttgcg
    20221 tttcagatta agcaaatccg cctgagcgct ttttgcttcc gggtgaactt ccggatacgc
    20281 cgccacggag atatcgaaat ctgccacttc ttttaatagc gtcaccaggt cagaagcata
    20341 catttctggc ttaccacttc ccggcggcag atcgccacgc agcgccacga tatgacgaat
    20401 accgttattc cagtagtcgc gtgcaatggt gcgcaactcg tcgggcgtcg catcaatgca
    20461 agtaagatgc ggtgccgctt ccagaccagt gcgatcttta atgcctttaa taatgctgtg
    20521 cgtacggtcg cgctcgccgg agttcgcgcc ataggtcacc gatacaaact tcggtttcag
    20581 gctgctaagg cgatcgatgg agttccacag ggtctgctcc atttcactgg tacgcggcgg
    20641 gaaaaactcg aacgaaacgt taatctgccc ctggacttct gccaggctct gattcagggc
    20701 atcccgctgg ctggcgtgaa aaaagctcat accttacctc atcaatcgct tgtcgttata
    20761 tgttgtgttg tgcacatcta tccgtttaga cgtccagatg taaagatgaa ggaaaagccg
    20821 aagggcgtca accgaaaaat gaccaatgat ggtgaggaaa aatcagggaa gatgaaaaaa
    20881 cttcaggatg gtaagaaaaa gaaaatccgg tatgcaaagc aaaccggact gcaaatatta
    20941 actaaccatg tacttactgc ttcttgccgg attcgtcgta aacgcttcat ccggcctaat
    21001 cctatgataa ttcaatatat ttcattagaa accatagacc cgaaaacatg agtaccgggc
    21061 attattaaat ttctgaaatt acaacaactg tgccagccgg ttgatatccg actgaatcgc
    21121 cccggcggtg acgtcgcgcc ccgcgccagg cccacggatc accagcgggt tgtcgcgata
    21181 ccagcggctt tcgatggcaa agacgttatc gcacggcagc agtgatgcca gcggatggtc
    21241 ttcacgcacc gcctccacgc ccacacgcgc tttgccgttg gcatcgaaac gcgccacgta
    21301 gcgcagcacc agccccattt cgcgggccgc ttccagccgt tgcaccatct gctcgttaag
    21361 ttcatcgccg ttttcaaaga agtggtcgat gctgccgcct tcgcaatggg caggcaccag
    21421 cgattccacg cgcacctgat ccggttcgat gtcgtaaccc gcttcacgcg ccagaatcac
    21481 cagcttgcgc atcacgtctt tgccggagag gtcgtcacgc ggatcaggct cggttaagcc
    21541 ctgctgccac gcctgatcca ccagctcggt aaacggcacg ctaccgtcga attgcaggaa
    21601 cagccaggag agcgtgccgg agaagatccc gctgatcgac aaaatagtat cgccgctgtc
    21661 gatcagatcg cgcacggtgt ggttgatcgg caagcccgca ccgacggtgg cgttgtacag
    21721 ccagtgacgc ccggtttttt cgaaggcgtc gtggatctgg cgatatttat tgctgtcgct
    21781 ggctcctgcc agtttgttgg cgctaatgac gtggaaaccg tggctggcga aatcgagata
    21841 ctgatcagca agctgctggc tggcggtaac gtccagcacc actaaatcat catacggatg
    21901 ggcgcgcatc cacaggaaaa gcgactcttc atcctgctcg accgcttcat cgttgaagaa
    21961 ggctaacgcg cggctggcgt ccagcccgtc ataactcaac agactgcggc ggctgtccac
    22021 cacgcctgcc agcacaaatt caaagccggt acgcgccgaa agcgtgctct gctcgcgggc
    22081 gaacagttcc agccagcggg aaccgatatt gcctttgccg aacaatacca ggccgatgcg
    22141 tttttctgcg cggaagacgg actgatgcag gccctgaatc aggctttcgg tcgggccggt
    22201 gcgcagtact gccaccaggc tgatgccgtc atcggactgc caggtaaact cgaccggctg
    22261 gcctttcagt tgctgccaga agcggtggca atgcagcggg ttacgggtga cacccgcccc
    22321 gaccatcgcc accagcgcca gcccctgacg caggcgcagt tcgccaggta atcccgcttc
    22381 gtcgaggatt ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt gcagcaactg
    22441 gcgatcgtta tgcacgccaa ccgccagtgg acgcacctgg gcacgtttca ggatctggtc
    22501 gatctcttta tgcgccagtt tgaaatcctg gctggtgggc acctgaaact caatcaagca
    22561 gacatcatcg tggctggtga caatacgcgc accagtaccg gaggccagca cgcgttcaat
    22621 gcgcgtggag ccttgatccg gcgtgtagct acagcgcagc tgcaggtcga tttcgctacc
    22681 ggatactggc tgtaaagtac gggcgtgaag aacgggagcc gccagacgcg ccagttcgct
    22741 ggcctcatcc agacgcagca acggcagcag acaggcatct ttcactttac gcggatcggc
    22801 actgtatacc ccggcgacgt cgctccagat ggttacacga gaaacacccg ccagcgcacc
    22861 gatttgtgtc gcggaatagt cggaaccgtt acgccccagc agcaccgttt caccggcgtt
    22921 gttacggctg ataaacccgg tcaccaccag acgtttaccc ggatgttgta ccagtagctg
    22981 ttgcagcaac gggtaagaaa gcccttcatc aacctgcggt tgcgcagcgc gttcagcgcg
    23041 taaaaactca cgggcatcaa gccaggccgc tggcagccct tgttgattaa gtaccgcaga
    23101 catcaggcgc gccgaccaca cttcaccgtg gcccaccact tccgcataca ctgcgtcgtt
    23161 aataccgctg tcgagcagcg ccgccaggcg ctcaagatcg ctgacaaaag cgctaatgag
    23221 gctatcggct tcttcggcgg gtagcaggcc gctaatcaga tcgcactgat agcgacgcag
    23281 cgtttgttga acctgatgcg cagagagacg atcggtctgg cttagtttca accagttaat
    23341 caactggtta gtggtgctac cggcggcgga aaccaccatc atatcgtcag gctgagagta
    23401 ctccgccata atgcccgcga cacgcaaata acacttcaca tcagccagac tactgccacc
    23461 aaatttatgc agctgacgac ctttcgcccc tgcctgcgca atcacactca tttttacccc
    23521 ttgtttgcag cccggaagcc attttccagg tcggcaatta aatcttcgcc atcttcaata
    23581 ccggtggaga tacgcagcag cgtctcggag atcccggcgg cagcacgcgc ttctggtgcc
    23641 atgcctgcat gtgtcatggt tgcggcgtga gagattaaac tttccactcc ccctaatgat
    23701 tccgccagcg taaacaacga cagcccgccc aggaaacgac gcagcgtctg ctcatcgcca
    23761 tccagttcaa aactcaacat tgcgccaaag cctttttgct gacgcgcggc aatttcatgc
    23821 ccctgatttt ccggcagcga cgggtgatac agttttttca ccaacggctg ggtttgcaga
    23881 tatttcacaa tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac cagcgttcgc
    23941 aacccgcgta gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc
    24001 caccaggcaa gttcagtgac aacgtccggg tctttagcaa tcaccacgcc ggccaccacg
    24061 tctgagtgac cgttcagata tttcgtacat gaatgcaaca ccagatcggc acccaatgcc
    24121 agcggatttt gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact
    24181 tcccttgcca gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg
    24241 ctttctacca gtaccagttt gggtttttct gccagcgctg cccgtaatgc ctgttcatcg
    24301 ccttgatcaa caaacaacac gcgatagcaa ccgcgtttcg ccaggctgtc gaacaggcga
    24361 tagctaccgc cgtagcagtc gtgcggcgca accagcagat cgccaggttt caaaaagacg
    24421 gtcgttacca ggtgaatcgc ggacatgccg gtattagtaa gtactgcacc agcaccgcct
    24481 tccagttctg ccagcgcacg ctgaaccaca tcgcgcgttg ggttgccgcg acgcgagtaa
    24541 tcatgcgcgc gcggttcatt aaatccggta aagttatagg tgctggaaag atggatggga
    24601 gggacaacgc aaccatactg ttcgtcgtca tttaacccgc tacgcactgc gatggtggcc
    24661 tgtttacgcg tcatgtgatg aagttccctg ggcttggtcg gtgaaatgtc aggcaccaga
    24721 gtaaacattg tgttaatgga cgtcaataca tctggacatc taaacttctt tgcgtataga
    24781 ttgagcaaat cccaaatagc cgttaaaatt atatgcatta tcacgccgac aggtgcatta
    24841 cacgatgtca cggtaacgcc tgtacggtaa actatgcggg tttacggtca gtacccacat
    24901 aaattgtgtg gtctggtctc aatttattga cgaagaggat taagtatctc atggctgaat
    24961 ggagcggcga atatatcagc ccatacgctg agcacggcaa gaagagtgaa caagtcaaaa
    25021 agattacggt ttccattcct cttaaggtgt taaaaatcct caccgatgaa cgcacgcgtc
    25081 gtcaggtgaa caacctgcgt cacgctacca acagcgagct gctgtgcgaa gcgtttctgc
    25141 atgcctttac cgggcaacct ttgccggatg atgccgatct gcgtaaagag cgcagcgacg
    25201 aaatcccgga agcggcaaaa gagatcatgc gtgagatggg gattaacccg gagacgtggg
    25261 aatactaaag cggaaaagag cggcgcggag ttaaccagcc gcttttttca tacaaggacg
    25321 tttaacgatg aaaatcaggc tactgatcct cagcctgctg gtttctgtgc ctgcctttgc
    25381 ctggcagccg caaactggcg acatcatctt tcaaatctct cgctcatcgc aaagtaaagc
    25441 gatccaactg gcgacccatt ccgattacag ccacaccggt atgctggtga tgcgtaacaa
    25501 aaagccctac gtttttgaag cggtcggccc ggtgaaatac accccgctca agcagtggat
    25561 tgcccatggt gaaaagggca aatacgttgt tcgccgcgtt gaaggcggac tcagtgttga
    25621 acaacagcaa aaactggcgc aaacggcgaa acgttatctc ggcaaaccgt atgacttcag
    25681 cttctcatgg agcgacgatc gccagtactg ttcggaagtg gtgtggaagg tttatcagaa
    25741 tgcgctggga atgcgcgtgg gcgaacagca gaagttgaaa gagttcgacc tgagcagccc
    25801 acaagttcag gcaaagctca aagaacgtta cggcaagaat attccgcttg aggaaacggt
    25861 ggtctcgcca caagccgttt tcgatgcgcc acaactcacc acggtcgcca aagaatggcc
    25921 gctgttttcg tggtaatgga tgataaaaaa aataattccc gccgtagtct gtttgttgct
    25981 ttgtagttgc acgacctttc cgcaagctgt cgctccggta aacggtgatg cctccaatta
    26041 gttgaattaa ggatataata ctcgctttta aggttgtttc atggtcacag ttaaccttca
    26101 ttgtccccgt tgccagtccg ttcaggttta ccgtcacggg cagaacccca aaagacatga
    26161 tcgattccgc ggccgtgact gtcaccgggt atttcagctc agttactgtt acgagccccg
    26221 taaaccggga gttaaagacc aaatcgctga aatggctttc aacggtgccg gggttcgcta
    26281 tactgcccgt acgctgaaaa tcggcattaa tacagtcatc cgaactttaa aaaactctcg
    26341 ccacgaagaa taacgtcatc cccggtcgct catgctgatg tggcactcat ctgcgaactt
    26401 agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc actggctctg gtacgtgtat
    26461 aacacgaaaa ctggtggtgt tctggcctac acttttggcc cccggactga tgaaacctgt
    26521 cgtgaattgc tggcgctgct taccctttta ccatcggcat gttgaccact gacgaatggg
    26581 gtagttacgc tagagagtta ccaaaggaga agcatctgac tggtaagatt tttactcagc
    26641 acattgagcg taacaatctg accctgagaa cccgaatcaa acgtctggcc cgcagaacca
    26701 tctgtttctc gcgttctgta gaacttcatg aaaaagtcat cggtgctttt atcgaaaata
    26761 tatgttctac taattggagt catcaccctt ctgatgagct ttccttggat gattaccaga
    26821 ctgaatacac agccaaagat ttttacctca ttctgctctt ttttctcaaa tgacatatat
    26881 tatattttac atcatattca taggcatatg acattccccc gccccccatt atgtcattgt
    26941 gggttgatgt tgggctttca taaaaaaata cat
//
LOCUS       DAZGCQ010000064        26885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000064 DAZGCQ010000000
VERSION     DAZGCQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.064"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..418
                     /locus_tag="ACHK07_003016"
     CDS             <1..418
                     /locus_tag="ACHK07_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO8103315.1"
                     /translation="KTLFKNTCFYNVRVKNVYLEGAYLNNDNIVKKANNSTEKQSIDS
                     TDKQANDSTVQQSIDSTDKQANDSTEKQANNSTEKQANNSTEKQANNSTDKQAKNSTE
                     QQDSNSSNQARLKKEVNIRFSIPGLTSYQPTYIVEE"
     gene            684..1526
                     /locus_tag="ACHK07_003017"
     CDS             684..1526
                     /locus_tag="ACHK07_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO8103316.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNANSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1594..2772
                     /gene="yjiJ"
                     /locus_tag="ACHK07_003018"
     CDS             1594..2772
                     /gene="yjiJ"
                     /locus_tag="ACHK07_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8103317.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            2983..8223
                     /gene="iatD"
                     /locus_tag="ACHK07_003019"
                     /pseudo
     CDS             2983..8223
                     /gene="iatD"
                     /locus_tag="ACHK07_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8347..8931)
                     /gene="kptA"
                     /locus_tag="ACHK07_003020"
     CDS             complement(8347..8931)
                     /gene="kptA"
                     /locus_tag="ACHK07_003020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO8103318.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            9181..9864
                     /gene="yjiH"
                     /locus_tag="ACHK07_003021"
     CDS             9181..9864
                     /gene="yjiH"
                     /locus_tag="ACHK07_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO8103319.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9861..10322
                     /gene="yjiG"
                     /locus_tag="ACHK07_003022"
     CDS             9861..10322
                     /gene="yjiG"
                     /locus_tag="ACHK07_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO8103320.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLTTAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10335..11507
                     /gene="iadA"
                     /locus_tag="ACHK07_003023"
     CDS             10335..11507
                     /gene="iadA"
                     /locus_tag="ACHK07_003023"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8103321.1"
                     /translation="MIDYNAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11572..12483
                     /gene="hypT"
                     /locus_tag="ACHK07_003024"
                     /gene_synonym="qseD"
     CDS             11572..12483
                     /gene="hypT"
                     /locus_tag="ACHK07_003024"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO8103322.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12476..12868)
                     /gene="iraD"
                     /locus_tag="ACHK07_003025"
     CDS             complement(12476..12868)
                     /gene="iraD"
                     /locus_tag="ACHK07_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO8103323.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLVISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12865..12948)
                     /gene="idlP"
                     /locus_tag="ACHK07_003026"
     CDS             complement(12865..12948)
                     /gene="idlP"
                     /locus_tag="ACHK07_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO8103324.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13064..13207)
                     /gene="ytiD"
                     /locus_tag="ACHK07_003027"
     CDS             complement(13064..13207)
                     /gene="ytiD"
                     /locus_tag="ACHK07_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO8103325.1"
                     /translation="MADYAEINSFPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            13540..14370
                     /gene="yjiC"
                     /locus_tag="ACHK07_003028"
     CDS             13540..14370
                     /gene="yjiC"
                     /locus_tag="ACHK07_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO8103326.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESEGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGIANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14511..15284)
                     /gene="uxuR"
                     /locus_tag="ACHK07_003029"
     CDS             complement(14511..15284)
                     /gene="uxuR"
                     /locus_tag="ACHK07_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO8103327.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15499..16959)
                     /gene="uxuB"
                     /locus_tag="ACHK07_003030"
     CDS             complement(15499..16959)
                     /gene="uxuB"
                     /locus_tag="ACHK07_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO8103328.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17040..18224)
                     /gene="uxuA"
                     /locus_tag="ACHK07_003031"
     CDS             complement(17040..18224)
                     /gene="uxuA"
                     /locus_tag="ACHK07_003031"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO8103329.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18564..19907
                     /gene="gntP"
                     /locus_tag="ACHK07_003032"
     CDS             18564..19907
                     /gene="gntP"
                     /locus_tag="ACHK07_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO8103330.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(20081..20983)
                     /gene="fimH"
                     /locus_tag="ACHK07_003033"
     CDS             complement(20081..20983)
                     /gene="fimH"
                     /locus_tag="ACHK07_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8103331.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPNYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(21003..21506)
                     /gene="fimG"
                     /locus_tag="ACHK07_003034"
     CDS             complement(21003..21506)
                     /gene="fimG"
                     /locus_tag="ACHK07_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO8103332.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(21519..22049)
                     /gene="fimF"
                     /locus_tag="ACHK07_003035"
     CDS             complement(21519..22049)
                     /gene="fimF"
                     /locus_tag="ACHK07_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO8103333.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(22059..24695)
                     /gene="fimD"
                     /locus_tag="ACHK07_003036"
     CDS             complement(22059..24695)
                     /gene="fimD"
                     /locus_tag="ACHK07_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO8103334.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVVCAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLSQLSAECR"
     gene            complement(24762..25487)
                     /gene="fimC"
                     /locus_tag="ACHK07_003037"
     CDS             complement(24762..25487)
                     /gene="fimC"
                     /locus_tag="ACHK07_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8103335.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(25524..26063)
                     /gene="fimI"
                     /locus_tag="ACHK07_003038"
     CDS             complement(25524..26063)
                     /gene="fimI"
                     /locus_tag="ACHK07_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8103336.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(26128..26682)
                     /gene="fimA"
                     /locus_tag="ACHK07_003039"
     CDS             complement(26128..26682)
                     /gene="fimA"
                     /locus_tag="ACHK07_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8103337.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         6829 a         6816 c         6441 g         6799 t
ORIGIN      
        1 aaaaacgctt tttaaaaata cctgttttta caatgtcaga gtgaaaaatg tctatcttga
       61 gggagcatat ctgaacaatg ataatatcgt gaaaaaagcc aataacagta ccgagaaaca
      121 atccattgac agtaccgata aacaagccaa tgacagtacg gtgcaacaat ccattgacag
      181 taccgataaa caggccaatg acagtaccga gaaacaagcc aataacagta ccgagaaaca
      241 agccaataac agtaccgaga aacaagccaa taacagtacc gataaacaag ccaagaacag
      301 taccgagcaa caggacagta acagttctaa tcaagcccgt ttaaagaaag aagtaaatat
      361 aagattttcc attccgggtt taacgtctta tcagccaacg tatatagttg aagaatagtt
      421 caataatcac ccaaacagag caggtatggc agaggatata aattaacatt taaacgttaa
      481 ccgcctcatc tatcattcat ggatgaggcg catttatcag tcgatgtaag ctgaaaatgt
      541 tctgccatcc agagacaacc gccgccccct gcggcgttta ctatttcatc gcccgcgtta
      601 agaattcatt aagcttcatc cgtatactcc ttatctgtcg cactgaattt gacgagaaaa
      661 aagcaataat aaggagcaat aagttgaaaa agcgcatcac ctttatcgtt tttctttgcg
      721 ccatcgtggc ggcaagcctg ttttttgtgc aatcctgcgt ccgtaaaagc cagcatgtcg
      781 cagggtttca gaattatcag gccactatcg atggtaaaga gattacagga gtaactaaaa
      841 atatctcctc actgacctgg tcagcgcaga gcaatacctt atttagcact atcaacaaac
      901 ctgcaaccat cgttgaaatg acgaccgagg gcgacctgat ccgcaccatt ccgctcgatt
      961 ttgttaagga tctcgagacg attgaataca ttggtgataa taaatttgtc atcagtgatg
     1021 aacgtgatta cgccatttat gtgatttcac ttaacgctaa ctcggaagtg agcatcctca
     1081 aaaaaattaa aatcccattg caggaaacgc ctactaactg tggctttgaa ggactggctt
     1141 actcccctca ggaccatacc ttctggtttt ttaaagagaa aaatccgata gaggtctaca
     1201 aagtaaccgg gctattgcgt agcgatgagt tgcatatcag caaagataaa gcgttgcagc
     1261 gtcagtttac tttagacgat gtttctggcg cggaatttaa cccacagaaa aatacgttgc
     1321 tggtgttgtc acatgagtca cgcgccctgc aggaagtgac cgtgagaggt gacgtgatcg
     1381 gcgaaatgtc gttaaccaaa ggtaagtatg gcctttcaca caacatcaag caggcggaag
     1441 ggatagcgat ggatgatagc ggcaacatct atatcgttgg cgaaccgaat cttttttatc
     1501 gctttacgtc gacaaaatca cgataacagt ctggcaggtt ctcctttttt gttattaact
     1561 tgttaactca aaataataca aaccaggcag aacatgaatt cgacatcgca tcccgtagaa
     1621 cgtttttctt tcagcaccgc gttattcggg atgctggttc tgaccttagg tatgggttta
     1681 ggccgctttc tctatacgcc gatgctgcca gtcatgctgg cggaaggcga gttttccttt
     1741 agcgaactct catggatcgc cagtggtaac tatgccgggt atctggcggg gagcctgctg
     1801 ttttcattcg gcgcgtttca tttaccctca cgcctgcgcc cgttcctgtt agcttccgcc
     1861 ctcgcaaccg gattattaat cctcgcgatg gcgtggctgc cgccgtttct tctggttttt
     1921 atcattcgct ttctggcggg agtcgccagc gccggaatgt tgattttcgg ctcaacgctc
     1981 atcatgcaac atacccgcca tccctttgtc cttgcggcgc tattttctgg tgttggcgtc
     2041 gggattgctc tgggcaatga atatgtgctg gcaggcctgc attttgccct ctcttcacaa
     2101 acgttgtggc aaggtgccgg agcactttct gccattatat tgcttgctct ggcgctgctc
     2161 atcccgtcga ataaacacgt tatcccgcca gcgccattgg caaaaatcgc gcaacaaccc
     2221 atgagctggt ggttactggc gattctgtat ggtctggcgg gttttggtta tatcatcgtc
     2281 gccacctacc tgccgctcat ggcgaaagac gcgggccagc ctgtgttgac ggctcacctc
     2341 tggacactgg ttggcttgtc gattgtccca ggttgctttg gctggctgtg ggcagccaaa
     2401 cggtggggag cattaccttg cctgaccgcg aatttgctgg tgcaggcgat ctgcgtgctg
     2461 ttaaccctcg ccagcagctc tcctttatta ctcatcatca gcagtattgg ttttggcggc
     2521 acctttatgg gaacgacctc gctggtgatg accatcgccc gccagcttag cgtgccggga
     2581 aatcttaacc ttttgggctt tgtgacactc atttatggta tcgggcaaat tcttggcccg
     2641 gcgctgacca gtatgctcgg caacggaacg tcggcgctcg ccagtgccac actctgcggc
     2701 gcagcggcgc tatttatcgc agcattaatc tgcgggatgc aaatattcaa attgcatacg
     2761 aattattctt aagtaattta acgaatttaa cttttcagta catcacgtaa aataattaaa
     2821 acatcctttt tctttggagg tactcagcca ccggcgttat tagtcctggt ttagcaattt
     2881 tacggctaat tcattatttc tggtcacatt atagtcagga tcgctttata tctccgtgct
     2941 agaaaaaaca agtttaattc tgttttttca cggaaggtca ctatggcttc gacaaatgcg
     3001 catcaaataa aaaataatga tcaaaactct ttatgtggtc tgggtgacaa aatacgccgc
     3061 ctgaccgccg gagtttgttt atttacacaa atttttttcc ccgtcatggc gacggcacaa
     3121 aatgtggtac atgctaaacc tcagacgaca gtttcatctg ccccccctct aatagaaaat
     3181 aatacggtgc cttacacgct tggtgcgctg gaatcagcgc aaagcgttgc tgatcgattc
     3241 ggtatttcac tggaggagct tcgtcgtctt aatcagttcc gtacttttgc tcgcggcttt
     3301 gataacgtgc gccagggtga ggaactggat gttccggcaa caacctcgca gaaaagccat
     3361 gagcaacaaa atgccatacc acctgcgaat ggcgaaaaca cgctggagaa tcaaatagcc
     3421 agcacctcgc agcgagttgg cactctgctt tcacaagata tgaatagtga gcaggccagc
     3481 ggcatggcgc gtggttgggc gtcttcagaa gcctcaggcg cgatgactga ttggttaaat
     3541 aactttggta ctgcgagaat ctctctgggt gtggatgaag attttagcct gaaaaattcg
     3601 caattcgact tcctgcatcc gtggtatgac acacctgatt atctgctctt cagccagcat
     3661 acccttcacc gaacagacga tcgtacccag atcaacaccg gtttgggctg gcgtcatttc
     3721 acctccagct ggatgtcagg catcaacctg ttttttgacc acgacctgag ccgctatcac
     3781 tcccgcgcag ggcttggcgc agaatactgg cgtgattatc tgaagttgag cagcaacgct
     3841 tatatcggcc tgaccggctg gcgtagcgca ccagaattgg ataacgactt cgaagcccgc
     3901 ccggccaacg gctgggattt acgcgcggaa ggctggttac ctgcctggcc acaactgggg
     3961 ggaaaactgg tctatgaaca atactatggc gatgaagtgg cgctgtttga caagaatgat
     4021 cgtcaaagta acccccatgc tattaccgca ggcctcaact ataccccctt cccgcttctg
     4081 actctcagtg cggaacagcg tcaggggaag caaggtgaaa atgacacacg ttttgccgtt
     4141 gatctgacct ggcaacccag cagttcaatg cagaaacagc ttaacccgga cgaagtggcc
     4201 ggacggcgca gtctggccgg tagccgttat gacctgattg atcgcaacaa caacatcgtt
     4261 ctggaatatc gcaagaaaga gctgattcgc ctgagtctgc tggatccagt gaaagggaag
     4321 tctggagaaa taaaaccgct ggtttcctcg ctacagacca aatatgccct gaaaggctat
     4381 aacatcgaag ccgctgcgct ggaagctgcc ggaggtaaag tcagcacgtc tggaaaagat
     4441 atcacggtca cgctgccagg ttaccgcttc actaacaccc cagaaaccga taatacatgg
     4501 tcgatagacg ttaccgccga ggatgtaaaa ggtaacctgt ctcggcatga acaaagcatg
     4561 gtagttattc aggctccgac attaagccag aaagattctc tattatccgt taatccgcta
     4621 accgtggctg cagataaaaa atcgacgacc acattgaccg ttactgcgca cgattccgac
     4681 ggaactccgg tgccggggct ggcgctgcaa acccgcagtg aaggcgttca ggatatcacc
     4741 ctgtctgact ggacagataa cggtgatggt agttacatac agatgctgac cgccggaacg
     4801 acatcaggtt cagtaacact gacgccgcaa attaacggtg agagtgcggt aaaagaatcc
     4861 atcgtcgtta atatcgtccc tgttgtctca tcccgcgacc attcatcaat aacaattgat
     4921 aacgtatcgt attatgccgg agacgacatc aaggttaggg tggaactgaa agacgaaagt
     4981 aatcaaccgg ttgcatatca aaaagaggaa ttggtaaaag ccgttactgt cgaaaacagc
     5041 aaacctggca ccacgattgt ctggcacgaa gagcagccgg gcgtttatac cgcgaattat
     5101 ccggcccata agcaagggac tgcactaagg gcacaactta gccttcacaa ctggaatgct
     5161 ccactgcaat cgcatattta taacattgag gcaaaccaga ataaggctcg cgtcgccaca
     5221 ttatcagcga caaataatga cgtttacgcc gataaaaaga catttaatac cctcactatc
     5281 aacgtcactg atgagagtga taatcccctg acaaatcatc aggtcacctt taagaatgaa
     5341 aaaggaagcg cggaatttgt cgaaccgccg cagcaaaata cggatgcata tggtgttgcc
     5401 acaatcaaca tggtaagtca ggttgcggaa gaaaatacga ttagcgccac gctgccaaat
     5461 ggtttctcac aacggataat tgcgaaattt gttagcgatt cgagtacgcc aaaattcaaa
     5521 caactggttg ccgatccaga taccattatt gctggcaaca gccagggcag tactctgacc
     5581 gccaccgtca cagactttca taacaacccg ttaaaagata tgaaagtgaa ttttgtggca
     5641 cctggtggct cgcaactgga caacacgtcc gccacaacag accagtccgg tattgtgcgg
     5701 gtgcacctga ccagttcaaa agctggtagc tattccgtcg atgcctcgct tgaggtggat
     5761 aaaaatattc accagtcggt cacgatcacc gtggtcccaa acagggaaca atcggtaatg
     5821 accttgaatg ccgggtcggg cagtgcgatc gctaacaata caaataccgt taccctgact
     5881 gccagtgtga aagatgttta tggacacccg ttgccggatg aggatgtgaa atttaccttg
     5941 ccagcctcca tgaccgggaa cttcacgcta agtagtgaaa ccgcccgcac cgatgcaaac
     6001 ggtgatgccg tggtcacatt gcgaggcaca aaagcgggtg agtttacagt tacggcgacg
     6061 ctgaccagaa ataataccgt tgcttatcag caagtcactt ttattgggga tacaaacagt
     6121 gcgcagctcc agccgctgac tgcctcatta aattccattg ttgcgggtaa cagtacgggg
     6181 agtaccctga cggcaacgat cctggacgct taccaaaatc cgcttaaaga ccagtaggtc
     6241 acattccaga gtaacgatgt cactctaagc ggaacagaag tcaccaccaa tacgctaggt
     6301 caggcgacgg taacaatgac cagcaatatt gccgggcaac ataacgtcgt ggtgagccgg
     6361 aaagcgcaag cctccgataa taaaacgttt agtttatcag tgctcccgga tgaaagttcg
     6421 gcgaaggtaa taagtataac cggagccgaa aaaacgataa cggttggcga aaacatcacg
     6481 ctacggatac tcgtccagga cgcgtttaac aatgtaatag cgggtcaacg cgtcagatta
     6541 agtgcgcagc caacagctaa cattacgata ggcgatacgg cttacaccga taataacggt
     6601 tatgcgtacg ttaaccttat tagcacccag cctggggttt atcaggtgac ggcaacgctg
     6661 gacaataaca gtagtagtaa ggttgacgtg aatgtggcaa atggcaaact cgagttaaca
     6721 tcatcgaaac cagaaaccac ggtccataat agtgagggta ttacgcttac cgcaacggcg
     6781 agaaatgcgc ggggtgaatt gatgccaggg caaattatca cctttagcgt aacgcctgaa
     6841 ggtgcaacgc taagcaatac aggggaagtc cttactgacc agtcaggtca ggccaaagtg
     6901 acgctgacca gtgacaaagt gaatgtctat accgttacgg ccataatggg caaagatgtt
     6961 cccgttcaga gccaggtaac ggttgcggtt aaggcagatg ctaaaacggc acatgttgtg
     7021 agcgtcgtgg cttctcctga caccatcacc gccgacggca tcgatagcag caccatcact
     7081 tcacgagtag aagatgatta cggattcccg gttgaaggtg tcgatgttag tcatggctta
     7141 gacaccaaag gcagcccggt agttaatatt ccaactacgc gtaccgatca gtccgggcaa
     7201 gtcacggcga caataaccag tacattggca gaaaccttaa cagtcaatgt gcaagttcct
     7261 ggtacagcca accaatccgc aaccattaca ttggttgccg gcacggccga tgaaagtaag
     7321 tcaattttga aatccgatgt tggcactctg aaggctgact accagcagag cgcaaaactt
     7381 acgctaacat tgcaagacaa gtacggtaac ccgatagtga cgtctgatca tctggaattt
     7441 gtccagtcag gccccttcgt gaactttctc aagttgagcg atattgatta cagccaaaga
     7501 aattatggcg agtacaccgt gactgtcact ggcggaaaag aggggacagc gaccctcatt
     7561 cccatgctga acggggttca tcaggcaaac ttaagcatat cgctgaatct catccaatcg
     7621 ataaaagaaa tgtccggtca tgtcactgca aacaaccata ccttctccac ggctaaattc
     7681 ccgagcgaag gctttgcagg ggcgtattac acactcaaca atgataactt tgaagcgggt
     7741 aaaaccgttg atgattatat gttttcaagt tcacagggtt gggtgtctgt cgatgcttca
     7801 ggtaaagttt ctttcgcaaa tatcggcgat caaacgtcag tcacaataag cgctgttccc
     7861 cgacaaggag gtacaaccta ccagacctta attaagctga aaggctggtg ggtgaataat
     7921 ggaaatcata ccaatatctg gctagctgcc aatgcgctct gtcatgctaa aaatgatgga
     7981 tataatcttc ctggcatcac acatttgacg tctggcgaaa acaaacgcac gcagggatca
     8041 ctgtatggtg aatgggggaa cgttggagcg ttttccagta attcgcaatt tacaccggga
     8101 gcttactgga caagtgaacc tgatgattac agtcggcact actatgtgca gatgctaacc
     8161 ggtatgaccg gaagcgacgc tgattccagc ccccaactga ccgcctgccg taaatcactt
     8221 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccttt cagactgata
     8281 acaaacgcta cattgccgga tgtgctgcgc ttatcaggcc tatatagccc ctgcaatata
     8341 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
     8401 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
     8461 catctcctgt gctttgacgg ttaagataac cggcgatccg tgtcttgctc ccactttgcg
     8521 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
     8581 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
     8641 tttttcggca aaggaaatag cgacctgcga ggtcgaatgc ccctgaaccg cgcggatgca
     8701 cctaccatca ctggaataac taaaacgctt tttatcgctg gtggcgacca cggtatccag
     8761 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tatcgatatc
     8821 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
     8881 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
     8941 gtccctggat tttctgtttt tatttaccaa tgtcattgtc actgggttta tgaattttct
     9001 gcaactcaat cctgcgtaga accttttcca cacagcgtaa tttttccgct atgcaaaaaa
     9061 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttcc
     9121 gctccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
     9181 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
     9241 gggtatctgg ctttttttct caccataatc tttttttccg gtgttttttc cggaacagac
     9301 agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt
     9361 gcgaacggag ccaccacctc atttcgcggc gcgggtggtg cgggggctaa agatggcttt
     9421 ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc
     9481 gatggtcttg gtggattacg cgccgcgcag cagttaatga cacctgtgct aaaaccgttg
     9541 ttgggcattc cagggatctg ctcgctggcg ctgatcgcta acttgcaaaa cactgatgcc
     9601 gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg
     9661 attttcgccg catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc
     9721 gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg
     9781 tttaaatttg tcggagccaa cattttgcgc gtctggctta atttcgaaga acgtcgcaac
     9841 ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta
     9901 tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga
     9961 tggcgtttgt catcattcag gcgctgaaaa taaccggcct gctcgactgg gtggggcata
    10021 tctgtgaacc cgtaatggcg ctgtggggat tacccggtga agcggcaacc gtccttttag
    10081 ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc cagtctgaca accgctggcg
    10141 cattaaccgg tcacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg
    10201 tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata
    10261 tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt
    10321 aacaaggagt taccatgatt gattataacg cagccggttt taccctgctg cagggagcgc
    10381 atttgtatgc gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta
    10441 tcgccgttgc cagcaatatc ccttctgaca ttgtaccgaa ctgcacggtt gtcgatctca
    10501 gtgggcagat cctctgccca ggttttattg atcaacacgt ccatttgatt ggcggtggcg
    10561 gcgaagcagg tcccacgacg cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg
    10621 gcgtcacgtc agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc
    10681 tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg
    10741 cttatcatgt cccttcccgc actattacgg gttccgtgga aaaagacgtg gcgattatcg
    10801 atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt
    10861 atcacctggc taatatggcg gcagaatccc gcgttggcgg tttgctcggc agtaaacctg
    10921 gcgtcaccgt gttccacatg ggcgacagta aaaaggcgtt acagcctatc tatgacctgc
    10981 tggaaaactg cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgtac
    11041 cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat atcaccagca
    11101 gcattgacga accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc
    11161 cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgatg
    11221 aagggaattt aacccatatc ggcgttgcag gttttgaaac gttgctggaa accgtgcagg
    11281 tgctggttaa agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg
    11341 tagccggttt ccttaacctg agcgggaaag gcgaaattct gccaggcaat gacgcagact
    11401 tactggtcat gacgccagaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg
    11461 tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc
    11521 gccctaatgg acgtagaatg cccgcaaggg cggctgacag agtaaaacgt aatggatgac
    11581 tgtggtgcgg ttttgcataa tattgaaacc aaatggcttt atgattttct gaccctggaa
    11641 aaatgccgca atttttccca agcggcagtc agtcgtaacg tctcgcaacc ggcattcagc
    11701 cgccgcatcc gcgcgctgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg
    11761 ccgctgcaac tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa
    11821 cagttagaaa gtaaccttgc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc
    11881 aagatagccg ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg
    11941 ccgccgctct ttacctgggc aattgaagcc attgatgtcg atgaagcagt cgataaactg
    12001 cgtgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg
    12061 tttgaccaca ttcgcttatt tgaatctcaa ttgttccccg tttgcgccag tgacgaacac
    12121 ggagaagcac tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac
    12181 tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc
    12241 accttttttg tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg
    12301 attgcctggc tgccggaata cgccatccaa caagaaattc gcagcgggca gctcgttgtg
    12361 ctgaatcggg acgaactggt gatcccgatt caggcctacg cataccggat gaatacccgc
    12421 atgaatcccg ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
    12481 tgacattctc aagcgtcgca ctgcgccaac cgccattatc ccagaccaga tcaaacgtta
    12541 gcggttcatc gtcgtggtac agcattatct caaggctgat caccagcgtg tggcagtacg
    12601 cttctttcaa attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
    12661 gcagctgacg gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact
    12721 cgggcatgcc tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga
    12781 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
    12841 aaacagcctg aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
    12901 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
    12961 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
    13021 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
    13081 tcatcaaact caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttacgtagat
    13141 gaaaatactt gtcaccgccg ctacttaatt ccggtgggaa actatttatt tccgcataat
    13201 cagccatgta ttatttgaaa caattagttt gtaaataata taaattgata acatatcaaa
    13261 tacatcaaaa ataccattca taggcattat taatatagac atcaatatag caacatcaaa
    13321 ttccaattag taataagata tgctttttaa ttgcatgaaa ataatttcaa atgagcgttt
    13381 tttgatactt tagagtgttt cctacccttt aatatcaccg tgtagagttg atatcactac
    13441 aacagaaaca tgcatgtcat ttttttactt cttttttact acagaaaaat ttatctattt
    13501 ttctaaacat cagtgaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
    13561 acccttcaga gccaaattat tacagataat cacttccttc atcatccaaa agtcgacagt
    13621 gagttaacac gtaaatatga acgtgcacgc ctggatacgg aaaatatcta tcttctgcct
    13681 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
    13741 gatattagta aagagtcctg gccatttaat tatgtgacag aagcatgcag agaatccgaa
    13801 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
    13861 gaaatatatg gtggcatatg taaaaaggcg catgctgcta cagaattagc agaaggttta
    13921 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
    13981 ataacattag gccctggttg caatgttcca ggttacgccg gaaccacgat aggatatatt
    14041 tcaacccttc cagtttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
    14101 tatattgatc agataataac tgtctcaggg atagcaaatt cttccggatt cgcccttgcc
    14161 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
    14221 cctggtaccg cggagatcca tgcaaaaatg ggatataacg ttatccccgg tgatgaagat
    14281 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
    14341 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
    14401 gttatacaga tatttaaaca acaccctgca atttacaaag taatactcat tgcgacttct
    14461 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
    14521 actttatcca gcggccatga atcaaacaga tagccgtcaa aataaatatc gtcaacgttc
    14581 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
    14641 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
    14701 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
    14761 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcggca
    14821 atggcgagat ggaactgcat atcaccgctt tcgctgctac ccggcgcgct ggaagccagt
    14881 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
    14941 agggcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
    15001 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
    15061 tcaagaacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
    15121 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
    15181 ggcggtagcc gttcaccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
    15241 ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta
    15301 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
    15361 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
    15421 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
    15481 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
    15541 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
    15601 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
    15661 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
    15721 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
    15781 gccgttttgc aggtgcagac gcaccggatc cagcagacgc tgcggtaact tctggctgcc
    15841 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
    15901 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
    15961 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
    16021 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
    16081 acgcagtttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
    16141 ccagtccgga cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
    16201 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
    16261 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
    16321 ccatgccgcc agctgcgggt cacgcgcctg agccagcccc agaaccgcga ccttcgccac
    16381 atgaccgttt tcacgcacgt tgtcgcagga catcaccgta aacgctttca accctttctc
    16441 acgacgcaga cgcaacgctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
    16501 ttccagatcg tgcttgatca gtgggttatt gagatccagt tgaccgcttg ccgcatcagc
    16561 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
    16621 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
    16681 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
    16741 tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
    16801 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
    16861 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
    16921 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
    16981 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtggatat
    17041 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
    17101 ttcagacgac caattgcgga gtaacctggg ttggttttct tcttcaggtc gtccagcatc
    17161 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
    17221 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
    17281 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
    17341 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
    17401 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
    17461 tgcatatctt caatggtgga aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg
    17521 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
    17581 aagacggcaa agttttcgcg cagcttcgct ttatcgatat ctttgtacag ctccaggtgc
    17641 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
    17701 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
    17761 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
    17821 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
    17881 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaataccg
    17941 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
    18001 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
    18061 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
    18121 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    18181 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    18241 catagtgggt tccacagcaa agagacgcgg aaccctggag ttgtttaggt atccggatga
    18301 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    18361 ggtaagacat gtcaacccga attctaaaat tggttaacca catcacaaga atttcactct
    18421 catggttgtt gctgcaaatt gcagcaaagg gtaattgatg gttattgatc agaggatgtg
    18481 acattcatcg caacaatggt tgaccaattt acataacata tcgcccaaaa taacaacggt
    18541 tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    18601 ttggtctgat gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    18661 tggcggcgct gtccgtcggg atgctggcgg gcatggattt gatgtcgctg ctgcacacca
    18721 tgaaagcggg cttcggcaac acgctggggg aactggctat catcgtggtg ttcggtgcgg
    18781 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcgcat acgctgctgg
    18841 cgcgtctcgg tctgcgctat gtacagctgt cagtgattat catcggcctg atttttggtc
    18901 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    18961 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    19021 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcag
    19081 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    19141 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    19201 cagatcaacc ggtagatatg aacaatctgc cctctttcgg cgtttcgatt ctggtgccgc
    19261 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    19321 cccctgcctg ggaagtggtt aactttatcg gttcctcgcc gattgcaatg tttattgcga
    19381 tggtggttgc attcgtactc tttggcaccg cacgtggtca tgacatgcag tgggtgatga
    19441 acgcttttga aagtgcggtg aaaagcattg caatggtgat tctgatcatc ggtgcgggtg
    19501 gcgtgctgaa gcaaactatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    19561 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    19621 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    19681 ttgatccagc aactggtcag ctgattggcg tgaatccggc gctgctggtg ctggcgacgg
    19741 ctgcgggttc caacaccctc acccacatta atgatgcctc attctggctg ttcaaaggtt
    19801 actttgacct gtcggtaaaa gacacgttga aaacctgggg actgctggag ctggtcaact
    19861 ccgtggttgg gctgattatt gtgttgatta ttagcatggt agcgtaaaag aacaccagag
    19921 cctgccaatg gcaggctcag ttcggattgt cggcaaagtg cgctttgttt atgtcagatg
    19981 cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg
    20041 taatattgcg tacctgcatt agcaatgccc tgtgatttct ttattgataa acaaaagtca
    20101 cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcgtaatttg
    20161 ccgttaatcc cagacttacc gccgaagttc ctactgctcc taacgatacc gtgttattcg
    20221 ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg
    20281 acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc
    20341 ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt
    20401 tcggcagagt aacggtgaca tcacgagcag aaacatcgca gccgccagta ggcaccacca
    20461 catcattatt ggcgtaaata ttccacacaa actggaaatc atcgctgtta tagttgttgg
    20521 tctgtcgcaa aataagcacg gcaattaatg agccagcttt aatcgccacc ccgcccgcac
    20581 tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa
    20641 caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg
    20701 tcccggaaaa attagataac acgccgccat aagccgagcc tcgttgcagt gtgacatagt
    20761 ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca
    20821 ggttttgccc cacattcacg gcaggcgcaa ggtttacata aacattagcg ctgccaccac
    20881 caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc
    20941 agcccatcag cagtacagca aacagggtaa taactcgttt cattacaatc atctcttcgg
    21001 gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg
    21061 ccatttaccg ttaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc
    21121 acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct
    21181 aactggatgt tttgcgcggt cccctggttt ttataatatc cggtactgtc ggctgccccg
    21241 ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca
    21301 tcatgccagg ccgatgccgc cccggcagac atcagactga aagaataaag atcgccgaga
    21361 tcaaccgtgg cattggtggt ggaaactgtg cacggtttgg cgacgacctt accgttcacc
    21421 gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat
    21481 aaatacccac gtttgcgcca tttcatgagc atctccagtt actgatattc aagagtgaag
    21541 gtagccgtgg cattgatatg ccccgcagtg acaggcacct gtgtcgccat tagccgggcg
    21601 taaaaattca acgtatttgg tttacccggc gtcagggtcg tccacgaaag cgcggacgat
    21661 ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgaa
    21721 gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacaccg
    21781 gtaaacccaa cttttacggc agaaacggca ttaccacagg gtgacagcaa aatacgaaat
    21841 ggaacgacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaaa
    21901 tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg
    21961 cggatagtaa tcgtgctatc cgcagccaaa gcgtgactca ccgccagcca caaaaaagcg
    22021 cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc
    22081 tggcttaata actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca
    22141 ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa
    22201 acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc
    22261 ccaaacggca gcggcttatt attgtgggtt agcgtcatga gcagttttat cccaacgcgc
    22321 gctttaaact ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta
    22381 tctaaatcga cgttatcagc cagggtattg gtatccagcg ccactctgtt ttcccgatat
    22441 tcggtggcat aaggcagcac ggcataaccg cgccagtcgg tacgcacccc cgtctggttt
    22501 tcgacttttg catcttttgc gccaggcgct ttaacaagca ccaccgtatc gtttaacggc
    22561 tgccccagcg ttacgccatt ggcatgagcc agtaccccac cgctgactcc gtaatagagc
    22621 tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa
    22681 ttcagcgtgg cgtagcctgt gcttccgcta ttaccatcgc ctcccccggc atagccggtt
    22741 tgcacgctat agctgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg
    22801 gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga
    22861 gatttgctgt cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc
    22921 tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata
    22981 tcttcgaacg cagtatttaa tccagcctgg aattgctcat cgacattact cgttccccaa
    23041 taagtttgat ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg
    23101 gtgagctgta atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc
    23161 ttaacctgaa taactccatc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt
    23221 gtatcagcga aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc
    23281 gtgcctgact cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga
    23341 ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc
    23401 cccatgtttt tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca
    23461 ccatatattg tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt
    23521 ttttcctgct gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc
    23581 ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg
    23641 tcagcctctt tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg
    23701 atggtaaaag gccccggtgg caccgtacta ttataaatgt catacccatt ttgtttaata
    23761 gtgacctgtg cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta
    23821 tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa
    23881 atatcgccct gggtataacc atcacccagc gtcagccggg aacgtaacgg tattatgtct
    23941 cgctcaagcc aggtattgat atgctgccat ttatttttgc tacctgatga actgtcgcta
    24001 ctgttataac tccagctggt attgtcgcgt aaacgccacg caccaatatt taacccactc
    24061 tgtaggttta aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg
    24121 ctgaaattat aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata
    24181 taaccacgcg cgcgattact cataaatgcc tgagggatcg tcaggttcag tcgctgctga
    24241 ccaacatcta aatgcgcagt agcgtcctgg accattgtgg ttaatggcac gcaggcatca
    24301 tccgccagca gattcatacc ggagacagaa gccgtattca gccccatact ggcaagttgc
    24361 gcgcgtgtca ggcagggaac aatcccttgt tcgctgtcgc ccgtattaaa tgtgacatca
    24421 cgcgttgcca tataaccatt attcaaatag atatcgacac gatacgtccc tggcggtaat
    24481 tcttgcccat tttcaaaacg cgataaatca gccacagcct ggggatcatc cgctaaaaag
    24541 cgcggattaa aatagagttc ggcagatgac aaaggtgtct gtgcggcaaa agcacagacg
    24601 acaacgagcc ggacaaaaaa accagccaaa cgatgcttac gaatatgcaa gcattgtgtg
    24661 tttcgctggt aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg
    24721 gcagtcaatt ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc
    24781 atttttgggg taagtgcacc ataatcattt attgttcggt aagtaatatt gcttcctgca
    24841 tcagaaggca atttaaccgt gctttcgccc attggaggca ccaatgcatt ttcaagaacc
    24901 cgggttccgg cattcaactc tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc
    24961 agagaattcg cgctacgacg aaatcttaat ttttctgcgg cctgatcggg tggcaacgct
    25021 aatttagccg gacgatagta cagtttaatg cggctgataa ttgcgagctg tagcgtattc
    25081 tcagtcaatt ttgatttatc cattgacgga atcgctttaa cgttcatcca gaataaactt
    25141 tcccggtcct gtggcaattg gttatttgtt gcatcaagaa tacgcaaggt attctctttt
    25201 tttcccttca tcgcaaacag aggaggcgtc acgataaaac gaccatcctt tacaccatcg
    25261 gcattttcca cccatgattg aattaaatag gtactatttt catcattatt tgtcacggca
    25321 agttgcactt gtttttgccc tgccggataa attacgcgag tcgcacctaa ggccactccc
    25381 gcttcagcgc gtccggcaac catcattgcc atgaacatca ggatacctgc cagcaagcag
    25441 aatgttattt cctgcgattt ccttacattg acgtttttat tactcactgt cctgttcctg
    25501 ttatcacatt atctgctgaa caattactga taggttaaag agaaccaggc ctgtgcattg
    25561 gcgatgccgc cagtaacccg acgcccggta gcacgatatt tggcgatgaa atgtagcgaa
    25621 gtagagcctg agtaaagccg tttccagttt gctggaggac gattaatcgg tacgaggttt
    25681 ccttcatcat caaacaacgc tacgccaata ttggtggcta tccctggccc ctctcccacg
    25741 gaaagcacat ccggattttt accatccgcg acaccgtgaa acgccacacc tacacgttca
    25801 ctcaccaccg tgctacattc ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc
    25861 cctaccgcat gaaaccggtt actgctgatt tgccccatat tgaccgtcat ttgtttatca
    25921 ccggcttcaa tccggcaagt ttccgcaata atgacgccct gaaattgcat atttccgccg
    25981 ggcagcgtgg tattccattt atttccggcc agagcaaaca ttggcagcaa cgacgctaat
    26041 agaaataatc tcttcctttt catcgttatt tttatcgcac aagggtgggc atccctgccc
    26101 gtaatgacgt ccctgaacct gggtaggtta ttgatactga accttgaagg tcgcatccgc
    26161 attagcagca cccggggttg cctcgccgat tgcataataa cgcgcctgga acggaatggt
    26221 gttggtaccg ttattcaagg ttgtttgtgc actaaatgtc gcaccgtcca gcgtcagctc
    26281 atttcctgtt ctgtccagta tctgcacacc aacatttgtt gcgctacccg cagctgaact
    26341 ctgcagagcc agtactttag gatgagcact gtcaatcgca gtccccaaga aggcaacagc
    26401 ggcttttgtg gcaacactgg tatcgcaatc attcagctga atattaaaac cgacagcaga
    26461 gctggttgct ccagtctgct tcagactagc ggtacgaacc tggcccaact gaacggtttg
    26521 atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac
    26581 ggtcccacca tttaccgtcg tgggtgtagt ttcagccaga gccgctgcgg aactgaggga
    26641 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttcaaaa
    26701 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
    26761 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
    26821 tagaggaaag cattgcgcac aaactttttc agttaatttg ttggcttaat attctattgt
    26881 tatct
//
LOCUS       DAZGCQ010000065        26726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000065 DAZGCQ010000000
VERSION     DAZGCQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.065"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..682
                     /locus_tag="ACHK07_003040"
     CDS             173..682
                     /locus_tag="ACHK07_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO8103338.1"
                     /translation="MNLQRFDDSTLIRIFALQELHRLKERGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            679..2097
                     /gene="phrB"
                     /locus_tag="ACHK07_003041"
     CDS             679..2097
                     /gene="phrB"
                     /locus_tag="ACHK07_003041"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO8103339.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHCPFIAWTERVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2247..3728)
                     /gene="dtpD"
                     /locus_tag="ACHK07_003042"
     CDS             complement(2247..3728)
                     /gene="dtpD"
                     /locus_tag="ACHK07_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO8103340.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3999..4742
                     /gene="ybgI"
                     /locus_tag="ACHK07_003043"
     CDS             3999..4742
                     /gene="ybgI"
                     /locus_tag="ACHK07_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO8103341.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4765..5421
                     /gene="pxpB"
                     /locus_tag="ACHK07_003044"
     CDS             4765..5421
                     /gene="pxpB"
                     /locus_tag="ACHK07_003044"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO8103342.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5415..6347
                     /gene="pxpC"
                     /locus_tag="ACHK07_003045"
     CDS             5415..6347
                     /gene="pxpC"
                     /locus_tag="ACHK07_003045"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO8103343.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDQLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6337..7071
                     /gene="pxpA"
                     /locus_tag="ACHK07_003046"
     CDS             6337..7071
                     /gene="pxpA"
                     /locus_tag="ACHK07_003046"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO8103344.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7107..7898
                     /gene="nei"
                     /locus_tag="ACHK07_003047"
     CDS             7107..7898
                     /gene="nei"
                     /locus_tag="ACHK07_003047"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO8103345.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7895..8941)
                     /locus_tag="ACHK07_003048"
     CDS             complement(7895..8941)
                     /locus_tag="ACHK07_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO8103346.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VNLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9090..10151)
                     /locus_tag="ACHK07_003049"
     CDS             complement(9090..10151)
                     /locus_tag="ACHK07_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8103347.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     PLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     APIDVGPFDTVVLFKINYN"
     gene            complement(10148..10879)
                     /locus_tag="ACHK07_003050"
     CDS             complement(10148..10879)
                     /locus_tag="ACHK07_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8103348.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKAASQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10894..13353)
                     /locus_tag="ACHK07_003051"
     CDS             complement(10894..13353)
                     /locus_tag="ACHK07_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8103349.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNSTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSTGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13403..13969)
                     /locus_tag="ACHK07_003052"
     CDS             complement(13403..13969)
                     /locus_tag="ACHK07_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8103350.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14356..15639)
                     /gene="gltA"
                     /locus_tag="ACHK07_003053"
     CDS             complement(14356..15639)
                     /gene="gltA"
                     /locus_tag="ACHK07_003053"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO8103351.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15845..15952)
                     /locus_tag="ACHK07_003054"
     CDS             complement(15845..15952)
                     /locus_tag="ACHK07_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103352.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16333..16737
                     /gene="sdhC"
                     /locus_tag="ACHK07_003055"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16333..16737
                     /gene="sdhC"
                     /locus_tag="ACHK07_003055"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8103353.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16731..17078
                     /gene="sdhD"
                     /locus_tag="ACHK07_003056"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16731..17078
                     /gene="sdhD"
                     /locus_tag="ACHK07_003056"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO8103354.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17078..18844
                     /gene="sdhA"
                     /locus_tag="ACHK07_003057"
     CDS             17078..18844
                     /gene="sdhA"
                     /locus_tag="ACHK07_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO8103355.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18860..19576
                     /gene="sdhB"
                     /locus_tag="ACHK07_003058"
     CDS             18860..19576
                     /gene="sdhB"
                     /locus_tag="ACHK07_003058"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO8103356.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19877..22678
                     /gene="sucA"
                     /locus_tag="ACHK07_003059"
     CDS             19877..22678
                     /gene="sucA"
                     /locus_tag="ACHK07_003059"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO8103357.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22693..23910
                     /gene="odhB"
                     /locus_tag="ACHK07_003060"
     CDS             22693..23910
                     /gene="odhB"
                     /locus_tag="ACHK07_003060"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO8103358.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            24186..25352
                     /gene="sucC"
                     /locus_tag="ACHK07_003061"
     CDS             24186..25352
                     /gene="sucC"
                     /locus_tag="ACHK07_003061"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO8103359.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25352..26221
                     /gene="sucD"
                     /locus_tag="ACHK07_003062"
     CDS             25352..26221
                     /gene="sucD"
                     /locus_tag="ACHK07_003062"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO8103360.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6489 a         6672 c         7270 g         6295 t
ORIGIN      
        1 ggtgaaatat gcagcggtac taccaatcgg tcaagtattc gctgctttga tgatgatgta
       61 aaatgattgt ttttgaactg gatataacag cttggtaggg aagaaagcat aaccccacaa
      121 aattcattta ccatcgtcta cacttactcc tgtaaaccgt tcaggagcag taatgaatct
      181 acaacgattt gatgacagca cccttatccg catttttgct ttgcaggagt tacaccgtct
      241 taaagagcga ggcttaacgc gcggggcgct tctcgattat cacagccgct ataaactcgt
      301 ctttctggcg cattcccagc cggaataccg caaacttggc ccgtttgtgg ctgatattca
      361 tcggtggcaa aatctggatg actattacaa ccagtactac caacgcgtaa ttgttttgct
      421 ttctcatcct gccaacccgc gagatcacac caacgttttg atgcacgttc agggttattt
      481 tcgcccgcat attgattcca cagaacgcca gcagctggca gcgcttatcg acagttatcg
      541 ccgtggcgag caaccgcttc ttgcgccgct gatgcgtatc aaacactata tggcgcttta
      601 tcctgacgcc tggctttcag ggcagcgtta tttcgaactt tggccgcgtg tgattaactt
      661 gcgccattca ggagttttat gactacccat ctggtctggt ttcgccagga tttacgtctg
      721 cacgataatc tcgcgctggc tgccgcctgc cgcaattcgt ctgcacgcgt gctggcgttg
      781 tatatcgcta caccacgcca gtgggcggcg cataatatgt cgccgcgtca ggctgaactt
      841 atcaatgctc aactgaacgg gctacaaatc gcgctggcgg aaaaaggtat tcctttattg
      901 ttccgtgaag tggatgactt tgtcgccagc gtcgaattag ttaaacaggt gtgcgcggaa
      961 aacagcgtta ctcatctttt ttataactat cagtatgagg tgaatgagcg ggcgcgggat
     1021 gtggacgttg aaagagcgct gcgtaacgtg gtgtgtgaag gatttgatga cagcgtgatc
     1081 ctgccgccgg gggcggtgat gactggcaat cacgagatgt acaaagtctt tacgcctttt
     1141 aagaatgcct ggctgaaacg gctgcgggaa gggatgccgg agtgcgtcgc tgcaccaaaa
     1201 gttcgtagta gcggatcgat aaagcccgcg ccatccatta cgctgaatta tcctcgtcag
     1261 tctttcgata ctgcgcattt cccggtggaa gaaaaagcgg cgattgcgca attacgccag
     1321 ttttgccaga acggtgccgg agagtatgag caacaacgag attttccggc agtggaaggc
     1381 accagccgtt tgtccgccag cctggcaacg ggcgggttat cacctcgcca gtgtttgcat
     1441 cgcctgttgg cggaacagcc gcaggcgctg gacggtgggg ccggtagtgt ctggcttaat
     1501 gagctgatct ggcgcgagtt ttaccgtcac ctgataacgt atcacccctc gttgtgtaaa
     1561 cattgtccgt ttattgcctg gacggagcgt gtgcagtggc agagcaatcc cgcacattta
     1621 caggcctggc aggaaggcaa aacgggatac ccgattgtcg atgctgccat gcgtcagctt
     1681 aacagcactg gctggatgca taacaggcta cggatgatta cagccagttt tctggtgaaa
     1741 gatttgttga tcgactggcg cgaaggcgag cgatatttca tgtcgcagct gattgatggt
     1801 gatttagcag ccaataacgg tggctggcag tgggcggctt ctacgggtac tgatgcagcg
     1861 ccgtattttc gtattttcaa cccgacaacc cagggcgaga aatttgaccg cgaaggcgag
     1921 tttatccgcc agtggctacc ggaactgcgc aatgtaccgg ggaaatcggt gcatgagccg
     1981 tggaagtggg ctgagaaagc aggtgtgaag ctggattatc cgcaaccgat agtcgagcac
     2041 aaagaggcga gagtacaaac gttggcagcg tatgaggcgg cgcggaaggg gaaataacag
     2101 acgcgtcagg caatcgagcc cagatgccgg atgcggcgtg aacgccttat ccagcctaca
     2161 aatttgtgca aattcaataa attgcaggaa atacgtaggc ctgataagac gcgtcaagcg
     2221 tcgcatcagg catcggtgct ggcctgttaa gactccagcg ccagcgcgcg gtttctgaat
     2281 ttcagcgcct gatacagcca aatcatcagt accactccta cacacgccag tgcgccccag
     2341 gtaatttgat caaatacttc aatatatgca ttgatggagt agttgatcgc cccggaagca
     2401 tcaaacgaag cctgcgatgt ctgatcggca atcacgcccg ccagatagtt cgcaatcgcg
     2461 ccggaaagca gcatataaat gccggttaat acgccggtca caccggggat ttcaatgcgc
     2521 gtaatctgcg acatagcaac cgggtcgata aacagttccg caaagcccat caccgccagg
     2581 cctaatacca tcagtggcag agaagagtga ccatacattg cggaccagcg ggcgcttaag
     2641 gtcagaatgc agaatccggc gctcatcagg ccaaggccaa gagcaaattt tccccagata
     2701 cgcacggtac gattacccgc gacactctct tttaccaccc acgccaggaa cacaccgcac
     2761 agcataactg cgaaggcatt aatcgactgg aacatcgcgg tcggaacggt ataaccaaac
     2821 atatcgcggt taacgaagcg gtcgatataa aggctaatcg agctaccgcc ctgttgtgcg
     2881 aaggcccaga acaacatact gaagaaggtg agcgtcacaa tcagccccag ctccttacgc
     2941 tgtttctggt tttctgcttt gcgataaatt tttgccagta cacccaggcc aatgatagtc
     3001 gcgacaatca aggcgtatac cgaccattct ttccagaaca gtacggtaat cagcgcaggc
     3061 gttgccacca gcagaaccag cagccatccc cagttcggca gcagaaagtt tgtcgcacgc
     3121 agtacttttt tgttaacgcc gcgggtatgt gtgaaatgac gattgccaca taagaaaata
     3181 accagacccg cgatcatacc caccgccgcc aggccaaagc ccatcgccca actgtactct
     3241 tcctgggcgt agccacaggc aataggtgcg ataatagacc ccacgttacc cgccgcatac
     3301 atcagcgaga aaccgccatc acgacgcgga tcggttggct catacagctc gccgagcagg
     3361 caactgacat tggatttaaa cagaccatag ccacaaacga ttatcgccag ggacagatag
     3421 aggaatgacg gatggatctc acttgcaccc aacaccacat gaccgatcgc catcaataac
     3481 gcccccagca tcaccgccat gcgattgccg agaactttat ccgccaaaaa gccaccgagg
     3541 attggcgtga catacaccag cgaacagtag gcgctaaata actcgtaggc gtgagtatcg
     3601 ttgtatttta gttgattggt gagatagaga atcagcaggg cacgcatgcc gtaaaagcta
     3661 aaatattccc agatttgcag cgcaacgaca tagtaaatag cgcgcggttg tgatgcgtgt
     3721 ttattcataa taattccaca attgacggca acgtcacgta atagttgcgc ggcgtgcttg
     3781 tgtttcctta aagttaaaac tttgatacag atctgattat ttatgcaata tgctgtctga
     3841 ttgcataaat atacattagc tgaagcgtga tgatataaac aatttgcgtc ttttggcgtc
     3901 gggtttgttt cttaatatgt caaaagattg gctatcgacg aacaggcgag gtaacgctat
     3961 gttaacggtg tagctatcag aatacggaag gcgcgaagat gaaaaacacc gaactggaac
     4021 aactgattaa cgaaaaactg aacagcgcgg cgattagtga ttacgcgccg aatggtttgc
     4081 aggtggaagg caaagagacg gtgcaaaaaa ttgttaccgg tgtcactgcc agccaggctt
     4141 tgctcgatga ggcagtgcgt ctgggcgcgg atgcggtcat tgtgcatcat ggctacttct
     4201 ggaaaggcga atctccggtc attcgcggca tgaagcgtaa ccgtttaaaa acgttgctgg
     4261 cgaatgatat caacctgtat ggctggcatt tgccgcttga cgcacatcct gagctgggca
     4321 ataacgcaca gctggcggcg ttactgggga tcacggtcat gggcgaaatt gagccgttgg
     4381 tgccgtgggg cgaactgacc atgcctgtgc cgggactgga gctggcttcc tggattgaag
     4441 cgcgtctggg acgtaagccg ttatggtgtg gcgataccgg acctgaggtg gtccagcgcg
     4501 tcgcctggtg cacgggcggc gggcaaagtt ttatcgatag cgccgcgcgt tttggcgtgg
     4561 atgcttttat tactggcgaa gtttctgaac agaccattca ttcagcccgc gagcagggat
     4621 tgcattttta tgctgcaggt caccatgcca ctgaacgtgg tggtattcgc gcattgagcg
     4681 agtggctgaa tgaaaatacc gatcttgatg tgacctttat tgatattcct aatcctgcat
     4741 aacgaataat cagagggatc gaaagtgcaa cgagcgcgtt gttatctgat aggtgaaacg
     4801 gcggtagtgc tggaactgga accgccggtg acgctggcta gccagaaacg gatctggcga
     4861 ctggcgcagc gtctggtgga tatgccgaat gtggttgaag ccattcccgg catgaacaat
     4921 atcacggtga ttttgcgtaa tcctgagtcg ctggcgctgg atgccataga gcgtttgcaa
     4981 cgctggtggg aggagagcga ggccctggaa cctgagtctc gctttattga aattccggtg
     5041 gtttacggtg gtgcaggcgg accggatttg gcggtggtcg cggcgcattg cgggttgagc
     5101 gaaaaacagg ttgttgaatt gcactcctcc gtggaatacg tggtctggtt tttaggtttt
     5161 caaccgggct tcccgtatct cgggagtttg ccggaacaac tacacacgcc acggcgcgct
     5221 gaaccgcgct tgctcgttcc ggcaggttct gtcgggatcg gcgggccgca gactggtgtt
     5281 tatccgctgg caacgccggg tggctggcag ttgattggtc atacctcact cagcctgttt
     5341 gatccggcgc gtgacgaacc catcttatta cgtccgggag acagcgtgcg ctttgtaccg
     5401 caaaaggagg gagtatgctg aagattattc gtgcgggcat gtataccact gtgcaagatg
     5461 gcggtcgtca cggttttcgc cagtcgggta tcagccactg cggcgcactg gatatgcctg
     5521 cgttacgcat tgctaacctg ctggtgggta atgatgccaa tgcccccgcg ctggagatca
     5581 cgctcggtca gttaacggtt gagttcgaaa ctgatgggtg gtttgctctg acgggtgccg
     5641 gttgcgaagc gcggctggat gataatgccg tctggaccgg ctggcgattg ccgatgaaag
     5701 caggccagcg tttaacgctt aaacgcccgc agcacgggat gcgcagttat ctggcggtcg
     5761 cgggtggtat tgatgttccg ccggtaatgg ggtcatgcag caccgatctc aaagtgggga
     5821 ttggcgggct ggaaggccgt ttactgaagg atggtgacca actcccgatt ggcaaatcga
     5881 agcgtgattt tatggaagcg cagggcgtta aacagctgct gtggggcaac cgcattcgcg
     5941 ccttgccggg gccggaatat catgagttcg atcgcgcctc gcaggatgca ttctggcgtt
     6001 cgccctggca gcttagcccg caaagtaacc gcatgggcta tcgcttacag gggcaaattt
     6061 taaaacgcac caccgatcgc gaactgttat ctcacggttt gttaccgggt gtggtgcagg
     6121 tgccgcataa cgggcagccc attgtgttga tgaacgacgc acagaccacc ggtggttacc
     6181 cgcgtattgc ctgtatcatt gaggctgata tgtaccatct ggcgcaaatt ccgctcggtc
     6241 agccgattca ttttgtccag tgttcactgg aagaggcact gaaagcgcgg caagatcagc
     6301 aacgttattt cgaacaatta gcgtggcggc tgcacaatga aaattgacct gaacgccgat
     6361 ctgggcgaag gctgcgccag cgacgcagag ctattaacgc tggtttcctc tgccaatatt
     6421 gcctgtggat ttcatgcagg cgatgcgcaa accatgcagg cttgcgtgcg tgaagcaata
     6481 aaaaatggtg tcgcgattgg cgctcatccg agttttcccg acagggaaaa ttttggtcgc
     6541 agcgctatgc agctgccgcc agaaaccgtt tacgcccaga cgctgtatca aattggcgcg
     6601 ctggcaacga ttgcccgtgc gcaaggcggc gtaatgcgtc atgtcaaacc gcacggcatg
     6661 ttgtacaacc aggcggcgaa agaagcacaa ctggcagacg ccatcgccag agcggtatac
     6721 gcttgcgatc cagcattggt tctcgttggg ctggcaggaa gcgagctgat tcgtgcaggc
     6781 aagcaatatg gcctgacaac gcgcgaggaa gtgtttgccg atcgcggtta tcaggctgac
     6841 ggctcgctgg tgccgcgaag ccagtcaggc gcgttgattg aaaacgaaga acaggcgctg
     6901 gcgcaaacgt tggagatggt gcaacacggc agagtcaaaa gtatcaccgg cgaatgggca
     6961 acggtcacgg cgcaaacggt ctgcctgcat ggcgacggcg agcacgcact ggcgttcgcc
     7021 cgccgactac gctctgcatt tgccgaaaag gggattgttg tcgcagcata accccgatta
     7081 ataaagaatg aaaaaaggat atcaccatgc ctgaaggccc ggagatccgc cgtgcagcgg
     7141 ataacctgga ggcggcgatc aaaggcaaac cactaactga tgtctggttt gccttcccgc
     7201 agttaaaaac ttatcaatca caacttatcg gtcaacacgt tacccatgtg gaaacgcgtg
     7261 gtaaggcgtt gttaactcat ttttccaacg acttaacgct ctacagccat aatcagcttt
     7321 atggcgtctg gcgcgtggtt gataccggcg aagagtcgca gaccacgcga gtattgcggg
     7381 taaaactgca aacggctgac aaaaccattc tgctttatag cgcctcggat attgagatgt
     7441 tgaccccgga acaactgacc acgcatccgt ttttacaacg cgttggtccc gatgtgctgg
     7501 atccgaatct gacgccggag gtggtgaaag aacggttatt gtcgccgcgc tttcgtaacc
     7561 gtcagtttgc tggattactg ctcgatcagg cgtttctggc tgggcttggc aattatttgc
     7621 gtgtggagat cctctggcag gttgggttga ctggaaatca taaagcgaaa gacctcaatg
     7681 cggcgcaact ggatgcactc gcacacgcgt tactggatat tcctcggttt tcttacgcta
     7741 cgcgggggca ggtggatgag aataagcatc atggggcgct gtttcgcttt aaggtttttc
     7801 atcgtgatgg cgaactgtgc gaacgctgtg gcggcatcat tgagaaaacc acgctgtcat
     7861 ctcgcccgtt ttactggtgt ccgggctgcc agcactaggc cgtacgtttc ggcgcatagg
     7921 ttgaaataaa ccgcgcaatg gcaggccctg tcagcaaaat actgaacagg cgcagggttt
     7981 gcatcgccat aatgagcgcc atatcggcat tgctccctgc ggcgatgacc gccacggtat
     8041 caagcccgcc ggggctggtg gcgaggtagg cagtcataaa atcaatgtgc ataaaccggg
     8101 tcagccccca cgccataccc gcacaaatag ccagcagagc aaaaatcgac agcaggattt
     8161 gtggtagcgg gcgtagtgcc cgcagtaaga tttgtttatc gaaaccaaga ccaatccgcc
     8221 agccaattgc catatacgcc atcgccagca gccattccgg tagttcgatg gtgatgagct
     8281 gaccagactg gagcactgcg cccgccagca tcgggatgag catcgtgccg gaaggaagac
     8341 gcagcaggca tcccactgta ccggcaacga ccgcgagcaa aatagtaagc aggagattaa
     8401 tgctcactgg cgggaaccag acgatatgct ggttaaccgc ttcagcgtta tcgcccagca
     8461 tcattcgcgt gaccagaact gcggcccccg cgacaaacag cactcgcaga tactgcataa
     8521 acgccaccag gcggatatct gcgccgtaat cctgcgccat cgcgaccatt gccgccgccc
     8581 cgccaggcga ggagccccag gcaccagtat ttccgggcag tgagctatag cgcaccagta
     8641 gccatcccac gatagcgctg gaaagcaacg tcaccagtaa aatagctaac acaatcggcc
     8701 agttaaccgc cagcgtggtc aggatcgaac cggtcaagtt ttgcgcaatc atgcagccaa
     8761 gaatggcctg cgcggcgaga aaagcggagc gggggagttg caggttaacc ccgcgcatac
     8821 tgaagatgat cccggcgatc atcggcccaa gtaataatgc cgccgggaga tgtaccgcga
     8881 ggaaaccaat agaaaggaga agtgatagca cacataacat tccccactgc aaaactggca
     8941 tcctttgctc cctcattatt tactctttgg tgtatcgata cagcataaag aatgccgggc
     9001 atggaaaaaa taacggtaat caataaaagc cggaaaattg gcaattaacc gaagaagccc
     9061 tctccggctg gaaagggcaa taggctaaat cagttgtagt taattttgaa aagtaccact
     9121 gtatcaaagg gaccaacgtc gatgggcgca cccggcgtgg atgacaattc tgccgtgtag
     9181 gttttttcat acactaacag gtcgctggta aaatcggcat actcactgta tttgttgaaa
     9241 gtatagggtg aggcattttc atccaacaat cgaagcttca ggccattgcc aatgagcagt
     9301 gatttatctt cgtcgataag gggttcatcg gtataaaacg aagagctgac tttaaaaccg
     9361 tcggtacact ggtcgttttg tgatttggtt gtagttattt taaattgctg cgacatcgta
     9421 tggcgacgaa tatccagcac gttaaatcgg ccaaaatcaa cgacctgggt ttcaggtgag
     9481 atagcaaagt tgacgctgca atccagcacg cggatatttt ccagaccggt aatgtgatat
     9541 ttcagattct tcgcggtagg gtcttcgttc acgctgcctg cgccatcaaa ctgtacgacg
     9601 atgtaatcgc tgagtttgct ctgataatca tggggcggca tggcgcgaac tttaacgtaa
     9661 agccgcatac gcgccacaaa cgtccgcgat ttgtgcataa cggttggatc gccggagcaa
     9721 agtttatttt gccagcccat cgcaatgatc tgctccgggg tgtaaatatc aatattctta
     9781 ctgtcgatgc actgattcgt gtcgatgcgg cttttcccaa ggcttgcgtc atagtccacg
     9841 ccttcatagg ttacgcccaa ttgataatag ggatcctgaa taccggggta agggtttacc
     9901 caggcgtaga cgtgctcgct ttcaaaatta ccgtttgtgt tgttgtcaca gtagaccgga
     9961 atttttatat cgtcagattc ccagatttta tcgccgggtt tggaattggc cggaacggcg
    10021 aagggcataa tagcttccga tttttctaca gcgccgtttg cggtaccaaa atagcagttc
    10081 agcgccagcg ccgcttttag cggcaaaaaa atcagacaga tggcgagcaa cacgcctttg
    10141 gcgaatctca ttttttattc tccaccggct gcaggcggca ttgccgctgg caggagagcg
    10201 tgttcatacg caatgcgccg tagtcgtcca tgtagccaag ataaaactgc ttgccgctat
    10261 agctgcccgt gctggtggtg accgaactga acggtgcgac catcgtgctt ttaaagccag
    10321 gcagcacgcc tttctcatcc cgcccaagat aggcaatagt cagatagtat ccggtcgggt
    10381 ttttcagggt aagctggtta ttctgctggc taacctgtag ctgttgctca acatgatcgc
    10441 ccattttttt gcgtaatgcg gcgggtcgcc agaatagctt aatgcgactt tggacggcga
    10501 cttgcaggat cgcatggccc tcgctgcctt ccgggacagg cggaatttca cgcaggttat
    10561 agtaaaagag agattcgcga tcggtgggta atttcgcggt ggcggcttgt ttcatgatcc
    10621 gaacctgcga agccgctttt ggctccagtc gctggatagg cggcagggcg acgagaaatt
    10681 catcgctttt ctctcccttt tcattttcta tccatgaata ggcgaggtag gggagtttat
    10741 cgctgcggtt ttccacgcgc aggctggttg ctttatcttt gctattaaag atgatgcgcg
    10801 tgcggtcagg ctgcactgcg ccgtggctgc acatactggc aaccagaaga atcaaaggga
    10861 atcctttaac aaatgtcatc gtcttttcct taattaatga cagggtaaaa tcaggcgctt
    10921 agtcacatct tccagatgtt ccggcagatg aatggcacac gttttctggt cgccccagtg
    10981 cacggtaaat tgttgatttt catcgacccc actcagccat gcgtgaccgt tttcgccaac
    11041 catcccgacg ctgacaccgc cttcagcctg gcgaacatcg gcaccgagcg gcgggaaatg
    11101 cccgttagca tcgctgataa tgacgttgag atctttcccg gcgcgggacg ccagcgattt
    11161 aaagccgatg gcgccttcgg tccaggtttc tttcaccacg ttttcggaaa cggtaacgcc
    11221 gtcaggcaag tcgttcatat tgaccgccac cgtcgtaggc tggtagctgg aaacaaacgg
    11281 taccacggca ataccaaagc ggttggtgta gtcaatattg ccctgaatcg gaatatcgcc
    11341 cacgccgtcg gtgctgacca tcaggcgtgg ttcattggtg gaactgcggc gatggaatgc
    11401 cgcgccatgt tgagtcgcgg taaacgagcc gctccaactg gcactggcgg aagtgtaatc
    11461 attggcggca taggttccgg tcaaatccca ttcccccgtc gaactgaggt gttggtaatt
    11521 gccgctgacc tgcgcaccgt tatccgggcg atcggattgt attccagccg acattcccca
    11581 actgttgcgt tcatccaggg tatcgttcca cgacatgcgg tgggtggtac tattactgtt
    11641 gttctgcatg tcataaccca gtcgaccact ttccccaatc ggtaaggaga tcgaaaagta
    11701 aatctggttg tcgcgatctt tatcttcata atgggtggtg ttgaacgagg tcgagacaga
    11761 aatatctttc cagtcgccaa tgtcgacgtt aaaaccggcg gtaatgttgg ccgtggtgga
    11821 ggtatccgca ttccaccagc tctgatgcag gatgttggcg tagagattaa gattcagcgg
    11881 agtaataggc tgaccgaagg agaggctgat ggtttgcttt tcatcctgcg cgtcggcgtc
    11941 attgtatttg tgatcaatgt agttagcgta gctgtgaaaa tggcgatcgg agaagcgata
    12001 ggctgccaat gagatggtgc tgttggtcgc atcaacctgt ttgctgtagt taaagcggta
    12061 gctgtaaccc tgctcatcct gttgagtatc aaaatggctg tcggcccagg tcacgtcaaa
    12121 cgacagcgcc cccagccaga gcatattttg cccaataccc agcgcgccgg aacgatagtc
    12181 atcgcctgcg agaagcatgc cgccatatag tgaagtgttg gagagcatcc cccaggagac
    12241 ttcatggcta atgaaggttt catcctcggt gtggtgcgac atagaggaac gcgggcgacc
    12301 ggcagccagt ttatagcgca cctggccctg acgcgtcagg aacggggtag aggccgctga
    12361 aacctggaag ttattcaccc gtccatcttc ttcgctcact ttgacatcca gtgtaccttg
    12421 cactgactga ttcaggtcat caataataaa tgggcctggc ggaacttttt tctggtaaat
    12481 cacgcgacca gaatggctta tcgtgaccgt ggcgttagtc tgtgcgatgc cgctgatttg
    12541 cggcgcgtag ccgcgtaact cccaggggag cattcgatcg tcgctggcta acgccgcacc
    12601 ggtataggag aaaccatcaa aaatgttaga gctaaaatcg gtttcgccca gggttaatcg
    12661 ggaacctatt tgcggtaacg ggcggaacag gtaggtccgt gaaatcgccc cggattgttc
    12721 acggttgtcg ccgctatcgg actggctaag ctgataatcg ctgcgcagac gccatgcccc
    12781 ggcgttgagg cccgtggtgc cataggcgtt caggttgttg ctgctgctgc cgctttgtgg
    12841 gcgataagtg ctggcaaaca ggttgtaatc catcaggaag ccaggaatac cgttattcca
    12901 cgttgagggt ggcgtccagt tttctgaatg ccacgccagc catgcctgcg ggattgtaat
    12961 attgagctgt tgatttgcct gatcgaaagt aaagatgatt tcagggaaga cactaaaatc
    13021 gacacattct tttattgccg gtaatttttt acgaaagtca gatttaagtc cgaagagttc
    13081 gatcagcgat tcattgatac aaggaataat tttatcgcca gatttttgcc agcgaatttg
    13141 ttgcccatta ctgattttat tattattaac cgtaacacta acaaagtaat cgccgggagc
    13201 tacgacgcct ttttgattga gcaacgatat atcaacgcta tcccgaatcg acttatcgag
    13261 aacgttaagg ttaaattcag ctgccagcgc aggaggggtt accattgcca gacaggaaag
    13321 aacagagagt cgataaatat tcacggcatc catacctgaa caatattcgc aaaaggcggc
    13381 taatcagccg ccttaaaaag gattattggt aatccagaac gtaagttgcg ttagcagtca
    13441 cggcccccgc ggtaaccgaa actgcgttat ccatctgttc catccaggca aagaagttta
    13501 acgtttgttc tgttgaactt gccaataagt caattgttgg cgcttctgtt cccaatacaa
    13561 caaatgattc atctttgttc atgagacgta cgccaacgcc ggtagcctcg ccagcactga
    13621 tgttactaag gagcggcgtt gcacctgtcg ttttagctgt gctgtcgaag gtcacgccta
    13681 cctttgagac cacgccaccg ttgccatcat cccagcctgc aatatcacag ttgattaagc
    13741 gaatattgac aggttttttc tcgctatgat gttctcgatt tatgtgggat gtggttactt
    13801 cacccaggtc aatgttcata tcaaggtcat cagggtgaat ttcacaaggt gcttcaataa
    13861 cttcaccttt aaagtggatt tcaccagatc cttcatctgc agcaaaaaca ggtgaagcga
    13921 atagtagaga tatggccagg gcggccagtg ttttttgtcc tttaaacata catgaatcct
    13981 tcatgggcta tgataaagta tactaattat tccgacagaa tagtggtttt ttgttcatgt
    14041 aataatatta atgttgctac attatgtttg agagtttcta aaagctctcg aatcataaat
    14101 aaagtgaatt aatcctatta cgagtttatt taattgtggg tgctacccaa tgtattctac
    14161 atcgattggc gggataacgg tggtggtgaa tgaaaaatta ggattttatt tagttatcgg
    14221 atatgtattc ttgtaacgtg taattatgta tttgtgtccg attacgtttt tttgtcgata
    14281 gacataacta acgaacaaaa aaaagcccgc cagatattca ggcgggctaa acgatatatg
    14341 gttcaaatca gataattaat gtttaacatc tgatttaaag tctcgttttt catagccggt
    14401 gtacaactgg cgcggacggg cgatcttcat gccgtcactg tgcatttcgc tccagtgagc
    14461 gatccagcca acggtacggg ccatagcgaa gatgacggtg aacatggaag acggaatacc
    14521 catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat
    14581 aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc
    14641 tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg
    14701 gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc
    14761 tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt
    14821 cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa tacctgctgc
    14881 gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc
    14941 gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg
    15001 attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata
    15061 ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc
    15121 tgccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat gacgaggatt
    15181 gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac
    15241 tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct gctcgtggat
    15301 catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc
    15361 accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat
    15421 cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc
    15481 gcaggatgcg gttgaagtga agccagggtc aaaggtgaac acaccttttg aaccgagagt
    15541 acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt
    15601 gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt
    15661 attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt
    15721 tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc
    15781 ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga
    15841 attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac
    15901 tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct
    15961 tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat
    16021 taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata
    16081 tgtaggttaa ttgtaatgat tttgtgaacg gcctatactg ccgccaggtc tccggaacac
    16141 cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt
    16201 ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg
    16261 aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat
    16321 aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt
    16381 aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc
    16441 gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct gggtaccagc
    16501 ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa
    16561 tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac
    16621 atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa
    16681 atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca
    16741 acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta
    16801 tcgtcctgac gctctacatc atttatatgg tcggtttttt cgctaccagt ggcgagctga
    16861 catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc accctgctgg
    16921 cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg accgactacg
    16981 ttaaaccgct ggctttgcgc ctgatgctgc aactggtgat tgtcgttgca ctggtggttt
    17041 acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt
    17101 tgatgcagtt gtgattggtg ccggcggcgc aggtatgcgc gcggcgctgc aaatttccca
    17161 gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc
    17221 tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca
    17281 tatgtacgac accgtgaaag ggtcggacta tatcggtgac caggacgcga ttgaatatat
    17341 gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg
    17401 tctcgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg
    17461 cgagcaggcg gcacgcactg cggcagcggc tgaccgtacc ggtcacgcac tgttgcacac
    17521 gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt atgcgctgga
    17581 tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg
    17641 tgaagtggtt tattttaaag cccgcgctac cgtgctggcg actggcggag cagggcgtat
    17701 ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca tggctatccg
    17761 cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg gcattgccgg
    17821 tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca
    17881 tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt
    17941 ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg
    18001 gccacacgcg aaactgaaac tcgatcactt gggtaaagaa gttctcgaat cccgtctgcc
    18061 gggtatcctg gagctttccc gtaccttcgc tcacgttgat ccggtgaaag agccgattcc
    18121 ggttatccca acctgtcact acatgatggg cggtattccg accaaagtta ccggtcaggc
    18181 gctgactgtg aatgagaaag gcgaagatgt ggttgttccg gggctgttcg cggtaggtga
    18241 aattgcctgc gtatcggtac acggtgctaa ccgtctgggc ggcaactcgc tgctggacct
    18301 ggtggtcttt ggtcgcgcag cgggtctgca tctgcaagag tctatcgccg agcagggcgc
    18361 attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga accgctggaa
    18421 caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca
    18481 gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg agcagttgaa
    18541 agttatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac
    18601 ccagcgcgtt gagtgtctgg aactggataa cctgatggaa acggcgtatg caacggctgt
    18661 ttctgccaac ttccgtaccg aaagccgtgg cgcgcatagc cgcttcgact tcccggatcg
    18721 tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg
    18781 ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta
    18841 ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt
    18901 tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc gcgacatgat
    18961 gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc
    19021 ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc
    19081 ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct
    19141 gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata
    19201 tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt
    19261 acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg
    19321 ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg
    19381 cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct
    19441 cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac agcatcatga actgcgtcag
    19501 tgtatgtccg aagggactga acccgacgcg cgccatcggt catatcaagt cgatgttgtt
    19561 gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat caggcaacca
    19621 gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt aggcctgata
    19681 agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact gcccctgaca
    19741 ctaagacagt ttttaaaggt tccttcgcga gccactacgt agacaagagc tcgcaagtga
    19801 accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga
    19861 tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact cttcttacct
    19921 ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa ccgatcctga
    19981 ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc
    20041 ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg
    20101 ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag tcctgcagct
    20161 cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg
    20221 gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg aagcagactt
    20281 ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga aactcggcga
    20341 gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt atatgcacat
    20401 caccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc gcgcgacttt
    20461 caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag gccttgaacg
    20521 ttacctcggt gcaaaattcc ctggtgcaaa acgcttctcg ctggaaggcg gtgacgcgtt
    20581 aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc gggaagtggt
    20641 tctcgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc
    20701 gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca cgggtgacgt
    20761 gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg tgcacctggc
    20821 gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg gctctgttcg
    20881 tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa tcaccatcca
    20941 cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc
    21001 gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg ttggtttcac
    21061 cacctctaat ccgctggatg cccgttctac accgtactgt actgatatcg gtaagatggt
    21121 tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg
    21181 tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta
    21241 ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc tgatgtatca
    21301 gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg agcaggaaaa
    21361 agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg cgctggatgc
    21421 tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca cctggtcgcc
    21481 gtatctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga agcgcctgca
    21541 ggagctggcg aaacgcatca gcacggtgcc ggaagcggtt gaaatgcagt ctcgcgttgc
    21601 caaaatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg actggggcgg
    21661 cgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc gcctgtcggg
    21721 tgaagactcc ggtcgcggta cattcttcca ccgccacgcg gtgatccaca accagtctaa
    21781 cggttccact tacacgccgc tgcaacacat ccataacggc cagggcgcgt tccgtgtctg
    21841 ggactccgta ctttctgaag aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga
    21901 accacgcact ctgactatct gggaagcgca gttcggtgac ttcgccaacg gtgcgcaggt
    21961 ggttatcgac cagttcatct cctctggcga acagaaatgg ggccggatgt gtgggctggt
    22021 gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg cgcgtctgga
    22081 acgttatctg caactttgtg ctgagcaaaa catgcaggtg tgcgtaccgt ctaccccggc
    22141 acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtcgt
    22201 gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg aagaactggc
    22261 gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga agggcgtgaa
    22321 gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacaac gtcgtaagaa
    22381 caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc
    22441 gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt gccaggaaga
    22501 gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag tgattccgtt
    22561 tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg tagggtatat
    22621 gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg tcgaataaat
    22681 aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga atccgtagcc
    22741 gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg tgatgaagtg
    22801 ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc agacggcatt
    22861 ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc
    22921 ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc
    22981 actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc gttaagccca
    23041 gcgatccgtc gcctgctggc tgaacacaac ctcgacgcca gcgccattaa aggcaccggt
    23101 gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc cccggcgaaa
    23161 gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc acgtagcgaa
    23221 aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa
    23281 aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat tatggatctg
    23341 cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc
    23401 ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa tgcttctatc
    23461 gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt ttctacgccg
    23521 cgcggcctgg tgacgccggt tctgcgtgat gtcgataccc tcggcatggc agacatcgag
    23581 aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt tgaagatctg
    23641 accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat gtctacgccg
    23701 atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg
    23761 gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc ctacgatcac
    23821 cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa
    23881 gatccgacgc gtctgctact ggacgtgtag tagtttaagt ttcacctgca ctgtagaccg
    23941 gataagacat tatcgccttc tccggcaatt gaagcctgat gcgacgctta acgcgtctta
    24001 tcaggcctac gggaagcacc gatgtaggtc ggataaggcg ctcgcgccgc atccgacaag
    24061 cgatgccaga tgcgacgctt acacgtctta tcaggcctac gggtgaccga caatacccgg
    24121 gagcgatacg aaatattcgg tctacggttt aaaagataac gattactgaa ggatggacag
    24181 aacacatgaa cttacatgaa tatcaggcaa aacaactgtt tgcccgctat ggcttaccag
    24241 caccggtggg ttatgcctgt actactccgc gcgaagcaga agaagccgct tcaaaaatcg
    24301 gtgccggtcc gtgggtagtg aaatgtcagg ttcacgctgg tggccgcggt aaagcgggcg
    24361 gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt tgcagaaaac tggctcggca
    24421 agcgtctggt aacgtatcaa acagatgcta atggccaacc ggttaaccag attctggttg
    24481 aagcagcgac cgatatcgct aaagagctgt atctcggtgc cgttgtagac cgtagttccc
    24541 gtcgcgtggt ctttatggcc tctaccgaag gcggcgtgga aatcgaaaaa gtggcggaag
    24601 aaactccgca cctgatccac aaagttgcgc ttgatccgct gactggcccg atgccgtatc
    24661 agggacgcga gctggcgttc aaactgggtc tggaaggtaa actggttcag cagttcacca
    24721 aaatcttcat gggcctggcg accattttcc tggagcgcga cctggcgttg atcgaaatca
    24781 acccgctggt catcaccaaa cagggtgatc tgatttgcct cgacggcaaa ctgggcgctg
    24841 atggcaacgc cctgttccgc cagcctgatc tgcgcgaaat gcgtgaccag tcgcaggaag
    24901 atccacgtga agcacaggct gcacagtggg aactgaacta cgttgcgctg gacggtaaca
    24961 tcggttgtat ggttaacggc gcaggtctgg cgatgggtac gatggacatc gttaaactgc
    25021 acggcggaga accggctaac ttcctggacg ttggcggcgg cgcaaccaaa gaacgtgtaa
    25081 ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa agccgttctg gttaacatct
    25141 tcggcggtat cgttcgttgc gatctgatcg ctgacggtat catcggcgcg gtagcggaag
    25201 tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa caacgccgaa ctcggcgcga
    25261 agaaactggc tgacagcggc ctgaatatta ttgcagcaaa aggtctgacg gatgcagctc
    25321 agcaggttgt tgccgcagtg gaggggaaat aatgtccatt ttaattgata aaaacaccaa
    25381 ggttatctgc cagggcttta ccggtagcca ggggactttc cactctgaac aggccattgc
    25441 atacggcacc aaaatggttg gtggcgtaac tccaggtaaa ggcggtacta cccacctcgg
    25501 cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct actggcgcta ccgcttctgt
    25561 tatctacgta ccagcaccgt tctgcaaaga ctccattctg gaagccatcg acgcaggcat
    25621 caaactgatt atcaccatca ctgaaggcat cccgacgctg gatatgctga ctgtgaaagt
    25681 gaaactggat gaagccggcg ttcgtatgat cggcccgaac tgcccaggcg ttatcacccc
    25741 gggtgaatgc aaaattggta tccaaccggg tcacattcac aaaccgggta aagtgggtat
    25801 cgtttcccgt tccggtacac tgacctatga agcggttaaa cagaccacgg attacggttt
    25861 cggtcagtcg acctgtgtcg gtatcggcgg tgacccgatc cctggctcta actttatcga
    25921 cattctcgaa atgttcgaaa aagatccgca gaccgaagcg atcgtgatga tcggtgagat
    25981 cggcggtagc gctgaagaag aagctgctgc gtacatcaaa gagcacgtta ccaagccagt
    26041 tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa cgtatgggcc acgcgggtgc
    26101 catcattgcc ggtgggaaag ggactgcgga tgagaaattc gctgctctgg aagccgcagg
    26161 cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca ctgaaaactg ttctgaaata
    26221 aatacttgta ataagaaata gccctcgccg cttccctcta caggaatggc gaagggctgt
    26281 cggtttcgac atggttggcc atcgtatgat ggcctttttt gtgccttaaa cgttaaccac
    26341 ggtctaaatc tgactgcagg cttgactgca tgaccacagt cactgtggat tgccgcaacc
    26401 gaccgcaagc cacgaataag aacgacggcg aatttcgaat cgttacctga tgcactggct
    26461 tcccagcgtt tgaccgtagc gttaagtaaa tcaacaaaag cgatgttcac cgcttttcgt
    26521 caagtcgtgc catttgtcag ccaggatcgg gtctttaccc cggatattgc agcatcgcaa
    26581 aaattcttag agggatatgc tataaatcaa aaataaaatc tttgttgatt gtttatcata
    26641 atatccacag atatgataaa tgatatcaat ttaataattt tcacttaatg tgatttcgga
    26701 taattcgaaa ggcgctgtgc ttttcg
//
LOCUS       DAZGCQ010000066        26537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000066 DAZGCQ010000000
VERSION     DAZGCQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.066"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..330
                     /gene="bhsA"
                     /locus_tag="ACHK07_003063"
                     /gene_synonym="ycfR"
     CDS             73..330
                     /gene="bhsA"
                     /locus_tag="ACHK07_003063"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO8103361.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(412..1374)
                     /gene="ldtC"
                     /locus_tag="ACHK07_003064"
     CDS             complement(412..1374)
                     /gene="ldtC"
                     /locus_tag="ACHK07_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO8103362.1"
                     /translation="MMIKTRFSRWLTFFTFATAVALALPVKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1518..4964)
                     /gene="mfd"
                     /locus_tag="ACHK07_003065"
     CDS             complement(1518..4964)
                     /gene="mfd"
                     /locus_tag="ACHK07_003065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO8103363.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEVINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQNDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREVLRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5092..6165)
                     /locus_tag="ACHK07_003066"
     CDS             complement(5092..6165)
                     /locus_tag="ACHK07_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO8103364.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVLQW
                     LALSALNQWLAPERDLSNASNAAYADSTGGFLHGMITASTSLWYLYALIVYFVLCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6427..7626
                     /gene="lolC"
                     /locus_tag="ACHK07_003067"
     CDS             6427..7626
                     /gene="lolC"
                     /locus_tag="ACHK07_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO8103365.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRFPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWHDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7619..8320
                     /gene="lolD"
                     /locus_tag="ACHK07_003068"
     CDS             7619..8320
                     /gene="lolD"
                     /locus_tag="ACHK07_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO8103366.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8320..9564
                     /gene="lolE"
                     /locus_tag="ACHK07_003069"
     CDS             8320..9564
                     /gene="lolE"
                     /locus_tag="ACHK07_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO8103367.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9593..10504
                     /gene="nagK"
                     /locus_tag="ACHK07_003070"
     CDS             9593..10504
                     /gene="nagK"
                     /locus_tag="ACHK07_003070"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO8103368.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10520..11341
                     /gene="cobB"
                     /locus_tag="ACHK07_003071"
     CDS             10520..11341
                     /gene="cobB"
                     /locus_tag="ACHK07_003071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO8103369.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11497..12543)
                     /gene="potD"
                     /locus_tag="ACHK07_003072"
     CDS             complement(11497..12543)
                     /gene="potD"
                     /locus_tag="ACHK07_003072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO8103370.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12540..13334)
                     /gene="potC"
                     /locus_tag="ACHK07_003073"
     CDS             complement(12540..13334)
                     /gene="potC"
                     /locus_tag="ACHK07_003073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO8103371.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13331..14188)
                     /gene="potB"
                     /locus_tag="ACHK07_003074"
     CDS             complement(13331..14188)
                     /gene="potB"
                     /locus_tag="ACHK07_003074"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO8103372.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(14172..15308)
                     /gene="potA"
                     /locus_tag="ACHK07_003075"
     CDS             complement(14172..15308)
                     /gene="potA"
                     /locus_tag="ACHK07_003075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO8103373.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15558..16784
                     /gene="pepT"
                     /locus_tag="ACHK07_003076"
     CDS             15558..16784
                     /gene="pepT"
                     /locus_tag="ACHK07_003076"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO8103374.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(16833..17954)
                     /gene="roxA"
                     /locus_tag="ACHK07_003077"
                     /gene_synonym="ycfD"
     CDS             complement(16833..17954)
                     /gene="roxA"
                     /locus_tag="ACHK07_003077"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO8103375.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18030..19490)
                     /gene="phoQ"
                     /locus_tag="ACHK07_003078"
     CDS             complement(18030..19490)
                     /gene="phoQ"
                     /locus_tag="ACHK07_003078"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO8103376.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19490..20161)
                     /gene="phoP"
                     /locus_tag="ACHK07_003079"
     CDS             complement(19490..20161)
                     /gene="phoP"
                     /locus_tag="ACHK07_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO8103377.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(20329..21699)
                     /gene="purB"
                     /locus_tag="ACHK07_003080"
     CDS             complement(20329..21699)
                     /gene="purB"
                     /locus_tag="ACHK07_003080"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO8103378.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21703..22344)
                     /gene="hflD"
                     /locus_tag="ACHK07_003081"
     CDS             complement(21703..22344)
                     /gene="hflD"
                     /locus_tag="ACHK07_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO8103379.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(22380..23486)
                     /gene="mnmA"
                     /locus_tag="ACHK07_003082"
                     /gene_synonym="trmU"
     CDS             complement(22380..23486)
                     /gene="mnmA"
                     /locus_tag="ACHK07_003082"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO8103380.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRVDVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(23540..24001)
                     /gene="nudJ"
                     /locus_tag="ACHK07_003083"
     CDS             complement(23540..24001)
                     /gene="nudJ"
                     /locus_tag="ACHK07_003083"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO8103381.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(24011..24664)
                     /gene="rluE"
                     /locus_tag="ACHK07_003084"
     CDS             complement(24011..24664)
                     /gene="rluE"
                     /locus_tag="ACHK07_003084"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO8103382.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            24836..26086
                     /gene="icd"
                     /locus_tag="ACHK07_003085"
     CDS             24836..26086
                     /gene="icd"
                     /locus_tag="ACHK07_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO8103383.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(26189..26512)
                     /locus_tag="ACHK07_003086"
     CDS             complement(26189..26512)
                     /locus_tag="ACHK07_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO8103384.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKPGIILKDKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
BASE COUNT         6436 a         6741 c         6911 g         6449 t
ORIGIN      
        1 cacatctatc atttagttat cgatcgttaa gtaattgctt gcgacgtcat tcatctgcat
       61 aaggccacta ttatgaaaaa cgtaaaaact ctcatcgctg cggcgatttt aagctccatg
      121 tcatttgcca gctttgcggc tgtcgaagtt cagtcaacgc cagaaggcca acaaaaagtc
      181 ggtacaatca gtgctaacgc ggggacaaat ctgggatcgc tggaagagca gctggcgcaa
      241 aaagcggatg agatgggcgc aaaatctttc cgtattactt ctgtaaccgg tccgaatacc
      301 ctccatggaa cagcagtaat ttataaataa gcattaaccc tcattaatgc ctgctactgc
      361 tgattttttc cccgcgacat gccgtgtcgc ggggattttt ttatccagga tttacagagt
      421 ttgtgggctc acttgatgac ggcggacatc caccggcatc ccggaacgga catccatcac
      481 atgttgcatc acttcagcgt cagtttgtgc tgcatcttta aatgattgca ttgcgctatt
      541 cagcgtaatt ggcagcagct gcggatcgtc atcaatcttc tctgacagcg gctgatgtac
      601 ttcaaccaga cgcgcaccgt ttggttcggc agagactttt atcggagtgt tgatgatatt
      661 cactttggtg cctggggtga cctggctaaa gagtgttttg atatcgtcat cccgcagacg
      721 aatacagcca gaacttaccc gcatgccaat gccgaaatcg gcgttcgtac cgtgaagcaa
      781 ataaacgccg ccataggccg ccagacgaat cgcatgatgg cccattgggt tatccggtcc
      841 agccggcact accgcaggca actcaattcc ctgtgcctta taccgcgcgc ggatgtttgc
      901 cgttggcgtc caggttgggt ttgctcgttt gtctgaaacg gtggtcacca ttgtcggtgt
      961 cagcgtgtca ccacccaact gaccaatacc tattggatac acggttaccg aatttttacc
     1021 cggcgggtag taatagagac gcagctccgc aatattgatc acaatgcctt cgcgcggcgc
     1081 atctggaagt agggtttgca acgggatcgt taacacgctg cccgcgcgcg gtacgtaagg
     1141 atcaacgccg gggttagcct gtaacagagc gagaaagccg acgttatatt ttttggcgat
     1201 ggcttccaga gaaccaccgt cattttccac cacatgaaat ttgttttcgc caaccagacg
     1261 actgcccgct ggcggcagcg gccaggtgtt ggcttttacc ggtagcgcca gcgccacggc
     1321 agtggcgaac gtaaaaaacg ttagccagcg agaaaaacgc gttttgatca tcaccaaaaa
     1381 tccataataa atataaggtt attgtaataa acagataagc ctgaattatg gatggtgaca
     1441 gtgtcggata gtgcagggaa gtgcaaagaa tttgtaaatg ttgcagatgg gggcgcagaa
     1501 acgcccccga tttaccatta agcgatcgcg ttctcttcca gttcacgcat aaactggcgt
     1561 acccattcga tacgcgtttt ccgctcactc aaatcctgaa taaatttcag gcgcgtcgga
     1621 ccatcaaggc ggtaatgctg cggctgtttt tgcagcaaac caatcaacca ggccggatta
     1681 acgtgattct tctcggcaaa ttcgatcacg ccgcctttct cattaccttc cagcttcctg
     1741 atccccagtt tctgcgcttg ctggcgcaga cgggcaatat ccagcagggt acgcgccgga
     1801 tccggcagca ggccgaagcg atcgataagc tcgactttga tctcttccag ttcgttttcc
     1861 gttttggcgc tggcaatacg tttgtagaac gacagacgcg tgttcacgtc agggatgaaa
     1921 tcatctggca atagcgacgg catccgcagc tcgacttctg tttgctggct ggtgagatct
     1981 tccagcgacg gctcgcgtcc ggctttcagt gcatcgacgg cgttttccag caactccata
     2041 tacagcgaga aaccgatggt ttccattgag ccgctttgtt cttcgccaag cagttcaccc
     2101 gcgccgcgga tctccagatc gtgcgttgcc agcgcaaaac ctgccccgag atcttccagc
     2161 gaggcaatcg cttcaagacg tttttgcgca tcggtagtca tcgcttttgg atgcggcgtc
     2221 agcagccatg catatgcctg atgatgtgaa cgtccgacgc gaccgcgcaa ctggtgcagc
     2281 tgcgccagac cgaagtgatc cgcgcgttca atgataatgg tgttggcggt agggatatcg
     2341 attccggttt cgataatggt ggtacaaacc agcacgttga aacgttgatg atggaaatca
     2401 ttcatcaccc gttccagttc gcgctcgcgc atctgcccgt gaccgatggc aatccgtgct
     2461 tctggcacca gttctgccag ccgttcggca gctttctgga tgttttccac atcattgtag
     2521 agataataaa cctgaccgcc gcgcagaact tcacgcagga tcgcctcccg gaccaccaga
     2581 ctgtcatact cgcggacaaa cgttttgacc gccagacgac gggcgggcgg cgtggcgata
     2641 atcgacaggt cacgcattcc gctcatcgcc atattcagcg tacgtgggat cggcgtagcg
     2701 gtaagcgtca gaatatccac gttcgcacgc atcgctttaa tgcgctcttt atgacgcacc
     2761 ccgaagcggt gttcttcatc gacaatcagc agacctaaat ctttaaactt gacgtcattt
     2821 tgcagcagtt tgtgcgtacc gatcagaata tcgattttcc cttccgccac ttccgcaagg
     2881 atttgcgttt gctctttggc gctgcggaaa cgggagatca tttcgatacg taccggccag
     2941 ttggcgaaac ggtcgcggaa gttgtcgtaa tgctgctgcg cgagaagggt ggtaggcacc
     3001 agcaccgcta cctgcttgtg gttatctact gccaggaaag cggcgcgcat cgccacttct
     3061 gttttcccaa agccaacatc gccgcacacc agacgatcca ttgccagcgg ctgacacatg
     3121 tcgctaagca ccgcattaat ggcctgcgcc tgatccggcg tggtttcaaa cgggaagctg
     3181 tcgcagaaca actgatactg ctcacgatcg tgtttaaacg cgaagccctc tttggcggcg
     3241 cgttgcgcgt agatatccag caattctgcc gccacatcac gcactttttc cgccgctttc
     3301 tgccgcgcgc gtgaccacgc atcgccgcca agtttatgca gtggggcgtt ttcttccgcg
     3361 ccacctgcgt aacggctaat cagatgcagt gacgacaccg gaacatacaa tttggcgtcg
     3421 ttggcatagg tgagcatcag gtactcgccg gtaatgccgc cagcttccag cgtggtcatt
     3481 cctgcatagc gcccgacgcc gtgctccaga tggaccaccg gctgaccaat gtgcaactcc
     3541 gcgaggttac ggatcagtgt atcggggttg atggtgcggc gagagtcctg acgacggcgg
     3601 gcaacgcgtt caccgagcag atcgctttcg caaatcagcg ccaggttacg caccttatcg
     3661 acaaaaccat gttcggcagc gccaatcatc aaataacgcc cacggtcgct ggcttcatca
     3721 agacgcataa tgcgttgcgg agcaatttta attcgcgcga gcagttcacc cagcgcttca
     3781 cggcgacctt cactttctac cgagaacacc accggaccgt cgaaagactc gaggaactta
     3841 cgcagcgcat ccagcggcgc tttttgttgt gcctgaacgg ccaggtctgg cagtttctgg
     3901 aaacctaaat tggcattcgc ggcttttgtc ggtaaatgtt cagtttttag ctgcactcgg
     3961 ggccagtttt tcagctctga gaagagctcg tccacccgca gccagagcga ttgtggtggc
     4021 aacagtgggc gcatcggatc gacgccgcga ttctcaaaac gcgccagcgt gtcagcctgg
     4081 aaacgttcgg cactgttttc cagatcgcca gtattcacca gcaaggtatt ggcagggaaa
     4141 taactgaaca gcggcggcag tggttcgctg aagaacaatg gctgccagta ctcgatcccg
     4201 gcaggtaatg tgcctttact cacttgctgg taaatatgtt ccggatcgcg cttcacttcg
     4261 aaggtatcgc gccactggct gcggaacagt tcaattgccg ctttatcggt cggaaattcg
     4321 tgcgcgggca gcagattgat cacttctact tcctccagcg tgcgctggct gtcgacgtca
     4381 aacacccgca ggctgtcgat ttcatcatca aagaaatcaa gacgataagg cagctcactc
     4441 cccatcggga agagatccag caacgcgccg cgcgtggcgt attcgccgtg ctccatcacc
     4501 tggtcaacat ggcgataacc ggcgctgtcc agttgggttc gtaatgcatc tcgtgacagg
     4561 cgctgacctt ttttcatcac cagcgcatga ccgtggagaa aactgtgtgg gcaaacgcgc
     4621 tgcataagcg tattcaccgg aacaatcagt acgccacgct gcatcgtcgg tagctggtaa
     4681 agggtggaaa ggcgcgagga gataatgtcc tgatgaggcg aaaaactgtc gtaagggagg
     4741 gtttcccagt ccgccagatt catcaccatc tgatcggtga actggctgat ttcatcatgc
     4801 aaacgcagag cattttgcat atctggtgca atgagtacca ccggaccggc gtgacgttcg
     4861 gcaatttccg ccaccagcgt cgcgcaggca gcgccggtta actcgcccag cagacgctgc
     4921 tcacccgctt tgacgggcag cgtataacga tattgttcag gcataacggt tgtcagattc
     4981 tcgttaggat atgcctcaac atatgggggc atatctctga taagcaatgc tgttcattat
     5041 ccgtaatcga catgctttag caaatcgaat cgccccggcg ggggcgatgg cttagcggga
     5101 gtaactgacg gcagcgggca gtgaaggcgg ggagaacaac agatcgctga atgcacgctg
     5161 tgaaagtttt cttaccagca agcccgcaac agtacagatg aacaaactga caaagggata
     5221 aaccagcagg agtgtcagtt cgacttgcgg cgaccagcgt gctgcgttca tttgcgcaag
     5281 cagagttaag ctgaatattt caaccagaat gcgatgggtg gtgtagatag caatggtgtt
     5341 ggagccaatc acattcagca ggctggtgga gcgcataccg aatcgttgct cgtattgata
     5401 aaacagcttc atgatcacca caatcgatac cagcgacaac agcagcgaga tagtaaacaa
     5461 ccaggcaccg accgccagaa cggtcagcaa agaagccatc agcagatggc ggcgcaacgg
     5521 cacctcttta acacaggtca ttattgttgc gccaaaccat gcgccaaggc tgtaataagg
     5581 caaattgcgg atcacactgt tcattcccca ccacggcgtg ggaacgaaat taaccgccac
     5641 actcagcagt acaaacaagg cgaatagtgg cagcgccaga cggctaaaaa ttttacataa
     5701 cacgaaatag acaattaacg catacagata ccacaagctg gtgctggcgg tgatcatccc
     5761 gtgcaggaac ccaccggtgg aatcggcata agcggcattg gaggcattgc ttaaatcgcg
     5821 ctcaggtgcc agccactggt tcagcgcact taacgccagc cactgcagca cgccccaaag
     5881 cgccagcacc cagaagatgt tccagatccg tttatcgaga caatttcccc acggtacgct
     5941 gtcgatatag cggcgaatca aatagccgga aataaagaaa aaaaccggca tacgaaaggg
     6001 ggcaaggtaa agattgaaat agatccagca tttgctcagg acttccgata acggatgctg
     6061 aaaagtggtc agatgcggat aaaaggtaat gaccgagtga taaatcacga ccagacaaat
     6121 acataaccct ttgatctggt taatccatag ctctttttgt ttcatcagga caacacgctc
     6181 ttttttaatt tacatgcggt tgatcctgcg ggttatgacc cgaggtgtaa tgggtatctg
     6241 tctgtattgg atgatttttc agattaagat caggcggcaa gattgatgat aaaacatggc
     6301 aatttagccg attgatttac gggggctttt cagattagcc ctgacgatca cttacagttc
     6361 agacgtttac ccatcctgct ttcgcttata tactcgtgtc tttgctacag caaccagacg
     6421 gatttcatgt accaacctgt cgctctattt attggcctgc gttacatgcg tgggcgtgca
     6481 gcggatcgct tcggtcgttt cgtctcctgg ctttctacca tcggcattac cctcggggtg
     6541 atggcgctgg tcacagtatt gtcagtgatg aacggctttg agcgcgagct gcaaaacaac
     6601 atccttggcc tgatgccaca ggcaattctt tcttctgagc atggctctct taacccgcag
     6661 caactcccgg aaacggcagt caaactggac ggcgttaatc gcgttgcacc tattactacc
     6721 ggtgatgtgg tactgcaaag cgcgcgcagc gtggcggtcg gggtgatgct gggtatcgac
     6781 ccggcgcaaa aagatccact aacgccgtat ctggtcaatg tgaaacaaac tgacctcgag
     6841 ccggggaaat ataatgttat cctcggtgaa cagcttgcct cacagttggg cgtcaatcgc
     6901 ggcgatcaaa tccgcgtgat ggtgccatct gccagccagt tcacgccaat ggggcgtatt
     6961 ccaagccagc gcctgttcaa tgtgattggc actttcgccg ccaacagtga agtcgatggc
     7021 tatgaaatgc tggtgaatat tgaggatgcc tcgcgtctga tgcgttttcc ggcaggcaat
     7081 attaccggct ggcgtttgtg gctggatgag ccgctgaaag ttgactcttt aagtcagcaa
     7141 aaactgcctg aaggcagcaa atggcatgac tggcgtgacc gtaaaggcga gctgttccag
     7201 gccgtacgca tggaaaaaaa tatgatgggc ttactgctga gcctgattgt cgccgttgcg
     7261 gcgtttaaca ttattacctc gctggggctg atggtgatgg agaagcaggg cgaagtagcg
     7321 atcctgcaaa cgcaaggctt aactccgcga caaatcatga tggtctttat ggtgcaaggg
     7381 gccagcgccg ggattatcgg tgcgatcctc ggagcggcgc ttggcgcact gcttgccagc
     7441 cagttaaata atctgatgcc gataatcggc gtcctgcttg atggcgcggc gctgccggtg
     7501 gctatcgaac ctttacaggt cattgttatt gcgctggtgg cgatggctat cgcgctgctg
     7561 tctacgcttt acccttcatg gcgcgctgcc gccacacaac ccgctgaggc tttacgttat
     7621 gaataagatc ctgttgcaat gcgacaacct gtgcaaacgc tatcaggaag gcagtgtgca
     7681 aaccgatgtg ctgcacaatg tcagtttcag cgtgggcgaa ggtgaaatga tggcaattgt
     7741 cggtagctct ggttccggta aaagtacctt gctgcacctg ctgggcgggc tggatacacc
     7801 aacctccggc gatgtgatct ttaacggtca accgatgagc aaactgtctt cggcggcgaa
     7861 agcagagctg cgcaaccaga agctgggctt tatttatcag tttcaccacc tgctgccgga
     7921 ttttaccgcc ctggaaaacg tggctatgcc gctgctgatt ggcaagaaaa agcccgctga
     7981 aattaacagc cgtgcgctgg agatgttaaa agcggtgggg ctggagcatc gtgcgaatca
     8041 ccgcccatct gaactttctg gcggcgaacg ccagcgtgtg gcgattgccc gtgcgctggt
     8101 caataacccg cgtctggtac tggcggatga acctaccggt aacctcgatg cgcgtaacgc
     8161 cgacagcatc ttccagttgc tcggagaatt gaatcggttg cagggcactg ccttcctggt
     8221 ggttactcac gacctgcaac tggcgaaacg tatgagccgc cagttagaaa tgcgtgatgg
     8281 tcgtttgacg gcagaactaa gcctgatggg ggcggagtaa tggcgatgcc tttatcgtta
     8341 ttaattggcc tgcgttttag tcgcggacgg cgacgcggcg gcatggtgtc gctgatctcc
     8401 gttatttcta ccattggcat tgccctcggc gtggcagtat tgatcgtcgg cttaagcgcg
     8461 atgaacggct ttgaacgcga actgaataac cgcattctgg cggtggtgcc gcatggcgaa
     8521 atagaggcgg tggatcaacc gtggactaac tggcaggaag cactggataa cgtgcaaaaa
     8581 gtgccaggta ttgccgccgc tgcgccgtat atcaatttca ccgggctggt ggaaagtggc
     8641 gcgaatctgc gcgcaatcca ggtgaagggc gttaacccgc aacaggaaca gcgtctgagc
     8701 gcattaccct cgtttgttca gggggatgcc tggcgcaatt ttaaagcggg cgaacagcaa
     8761 attatcatcg gcaaaggcgt ggcggatgcg ctgaaagtga agcagggcga ttgggtgtcg
     8821 attatgatcc ccaactcgaa tcccgagcat aaactgatgc agccaaaacg tgtgcgtttg
     8881 cacgttgccg gtattttgca gttgagtggt caactcgatc acagttttgc catgatcccg
     8941 ctggcggatg cccaacaata tcttgatatg ggttccagcg tgtcaggtat tgcccttaaa
     9001 atgacggatg ttttcaacgc caataagctg gtacgcgatg cgggtgaagt gaccaacagc
     9061 tatgtttata ttaaaagttg gattggtact tacggctata tgtatcgcga tatccagatg
     9121 atccgcgcca ttatgtatct ggcgatggta ctggtgattg gcgtggcctg tttcaacatc
     9181 gtctccacct tagtgatggc ggtgaaagac aagagtggcg atatcgcagt attaagaacg
     9241 ctgggggcga aagatggttt aattcgcgcc atctttgtct ggtatggatt gctggcaggg
     9301 ctgttcggca gcctgtgtgg ggtgattatc ggcgtggtgg tttcactgca acttaccccg
     9361 attattgagc ggattgaaaa gctgatcggt catcagttcc tctccagcga tatctatttt
     9421 attgacttcc tgccatcgga attgcactgg ctggacgtct tctacgtact ggtcacagca
     9481 ttgttgctga gtcttttggc aagttggtat ccggcgcggc gcgccagtaa tattgaccct
     9541 gcgcgagtcc ttagcggcca gtaaaggcag tacattaaaa caaggagcgg caatgtatta
     9601 cgggtttgat attggtggaa caaaaattgc gcttggcgtg tttgatagcg gtcggcagtt
     9661 gcagtgggaa aagcgggtgc cgacaccgcg tgacagctat gacgcatttt tagatgcagt
     9721 gtgtgagctg gtagctgaag ctgaccaacg ttttggctgt aaaggttctg tcggcatcgg
     9781 tattccggga atgccggaaa cagaagatgg tacgctgtat gccgccaatg tccccgctgc
     9841 cagcggtaaa ccgctgcgtg ccgacctgag cgcacgtctt gatcgcgatg tccgccttga
     9901 taacgatgct aactgttttg ccctttcaga agcctgggat gatgaattta ctcaatatcc
     9961 actggtgatg gggttgattc tcggcaccgg cgttggcggc gggctgattt tcaacggtaa
    10021 accgattacc ggtaaaagct atattaccgg cgagtttggc catatgcgtc tgccggttga
    10081 tgcgttaacc atgatggggt tggatttccc gttacgccgc tgcggctgtg gtcagcatgg
    10141 ctgcattgaa aattatctgt ctggtcgcgg ttttgcgtgg ctgtatcaac actattatca
    10201 tcaaccgttg caggctcccg aaattattgc gctttatgat caaggcgatg agcaggcaag
    10261 ggcgcacgtt gagcgttatc tggatttatt agcggtttgt ctgggaaata tcctgaccat
    10321 tgttgaccct gacctggtcg tcattggtgg tggcttatcg aatttcccgg caatcacaac
    10381 gcaactggcg gacaggctgc ctcgtcatct cttacctgta gctcgtgttc cgcgcattga
    10441 acgcgcgcgc cacggtgacg cgggaggaat gcgtggtgcg gccttcctac atctaaccga
    10501 ttaaacaaca gaggttgcta tgctgtcgcg tcggggtcat cggttaagtc gttttcgtaa
    10561 aaataaacgc cgcctgcgcg agcgtttgcg tcagcgtatt tttttcagag ataaagtggt
    10621 gccggaagca atggaaaaac caagagtact cgtactgaca ggggcaggga tttctgcgga
    10681 atcaggtatt cgtacctttc gcgccgcaga tggcctgtgg gaagaacatc gggttgaaga
    10741 tgtggcaacg ccggaaggtt tcgatcgcga tcctgaactg gtgcaagcgt tttataacgc
    10801 ccgtcgtcga cagctacagc agccagaaat tcagcctaac gccgcgcatc ttgcgctggc
    10861 taaactgcaa gatgctctcg gcgatcgctt tttgctggtg acgcagaata tagacaacct
    10921 gcatgaacgc gcaggtaata ccaatgtgat tcatatgcat ggggaactgc tgaaagttcg
    10981 ttgttcacaa agtggtcagg ttctcgactg gacaggagac gttaccccag aagataaatg
    11041 ccattgctgc cagttcccgg cacccttgcg cccgcacgta gtgtggtttg gcgaaatgcc
    11101 actcggcatg gatgaaattt atatggcgtt gtcgatggcc gatattttca ttgccattgg
    11161 tacttcgggg catgtttatc cggcggctgg gtttgttcac gaagcgaaac tgcatggcgc
    11221 gcacaccgtg gaactgaatc ttgaaccgag tcaggttggt aatgaatttg ccgagaaata
    11281 ttacggcccg gcaagccagg tggtgccaga atttgttgaa aagttgctga agggattata
    11341 aagcaggaag cattgcctga tgcgctacgc ttatcaggcc tacaagcgga ccacaatacc
    11401 gacgaaagag cgggataaaa ctttactgtc ctgactcatt agccacatcc ttgctaacta
    11461 aaaaacgggc ggtaatacca ccgcccgctt gctgaattaa cgtcctgctt tcagcttctg
    11521 ataatactct tcataaatgc tgctggctgc gccaacgtca ttctgccatt cgccattttt
    11581 aatggtttca gcatccgggt agagcgtctt atcgttcgcc acttctggac ttaacagctt
    11641 acgcgccgca aggtttggcg ttggataacc gatagtttca gcaacctgtt ttgccacatc
    11701 cgggcgcagc aggaagttga tcaatttcag cgcgccttct ttgtttttgg catttgccgg
    11761 gatcgccagg ctgtccatcc agaatatgcc gccttctttc ggccacacca cgtcaatagg
    11821 cgtacccgcc tggcgtgcaa cgaaagcaga accgttccag atcatgccga ggttaacttc
    11881 gccttccatg tacgggttag ccgggttatc ggagttaaac gctgcgacgt ttggcatcag
    11941 ttttttcagc tcgttatatg cagcttcaat ctctttcgga tcggtggtgt taccggagta
    12001 gcccagctta cgcagcgcca tctggaacac ttcacgggca tcgtcggtca gcagcaggct
    12061 gcctttgtac tcaggcttcc acagatcggc ccagctggta acggatttcg gatctaccgc
    12121 atcaccgtta acgccaatcg ccgtcgcccc ccagatatac ggaatggagt agtcgttatt
    12181 cgggtcaaac ggcttgttga gcatgtctgg atcgaggttg ctgaaattgc ttaacttcga
    12241 cttgtcgatc ttctggatca tcccttcttt acgcatttta tcgacgtaat aggttgaagg
    12301 aaccaccagg tcgtaggcac cgtctttgta cgttttcagc ttggcgtaca tggtttcgtt
    12361 cgactcgtaa gtcgaataga taaccttaat accggtttct ttggtgaact gttcaagcag
    12421 tcctggcggc acgtactcgg tccagttgta gaaatacagc gtgttgttgt catcggcgtg
    12481 agcggcgctc atgcccagtg ccagagcacc cgccgcgagc aggtggcgtg accatttttt
    12541 catttaacgt cccctgtgtt acctttcgtt ttatcacgag caataagctg gctggcaata
    12601 accatcacca gcgacagcac cagtaatatg gttgccagcg cgttaacttc cggcgatacg
    12661 ccaactttga ccatcgaata aatttttaac ggcagaattt cataacttgg tccggtgacg
    12721 aacgaagaaa ctaccacatc gtccatcgac agggtaaagc ttaacaccca gcccgccgcc
    12781 accgctggca ttgccagtgg cagaatgatt tttcgcagaa tggtaaattc gctggcaccg
    12841 agatctttcg ccgcttccag cattcgcacg tcaaaacctt tcaggcgcga atacaccgtc
    12901 accaccacaa atggcaggca gaaggtgata tgcgagaaca gcagcgacca gaagccaagc
    12961 tgaataccca gcagcataaa cagcaccagc agcgaaatcg ccatcacgat atctggtgac
    13021 atcatcacca caaacagcat tccgctaacg aacggcttac cacgaaagcg ataacggtac
    13081 agcgcaactg ccgtcagtga accgataagc gtagcaaacg tcgccgaaaa caccgccatt
    13141 gtcagtgaat gctgcgctgc ctgtaacagg ctgtcgttgt tcatcagcaa gctataccat
    13201 ttagtggtaa aaccctgcca gttgatgcca aagcgcgagc tgttaaagga gttcacaatc
    13261 aaaataatga ttgggatata caggtacgcg tagatagcgg tcataaaacc gccgcgaagc
    13321 agtcgaccga tcattcgagt tccaccttct tattcagcag acgagaagcg cgccagtaaa
    13381 ccagcaacat caggcccatt acgatagtca gcgtaatgct ggtggctgca ccaaacggcc
    13441 agtcacgaat attaaggaac tggaccttga tgacgttacc gatcagcagg tttttcgcac
    13501 cgcccatcag gtcggacaca tagaacaggc ccatcgctgg cagcatcacc agcagacatc
    13561 cggcaataat ccccggcatc gtcagtggaa taatgatacg gataaaagtc tgtaacttgc
    13621 tggcaccgag atcgcgcgcc gcctcaagca acggcttatc cagtttttcg atactggagt
    13681 acagcggcat caccataaac ggcagcagaa tgtaaaccag accgataatc accgcactgg
    13741 gcgtgaacat gatacggatt ggtgtgtcga taacgcccag ccagagcaaa aactcgttga
    13801 ggtagccttt ggtgctgagg aaaattttca gcccgtagat gcgaatcagt gagttggtcc
    13861 agaagggaac aatcagcaga aacagcagca gcggacgcac cttgtgtggc agcttcgcca
    13921 gaaaccaggc aaacggatag cccagcacca ggcaggcgag ggtggcgatc agcgccatat
    13981 tcagcgagtg caatagcact tcaaaataga gcggatcgag cagacgtgtg tagttatcca
    14041 gcgtaaagac cattttgacg aaactggcgt cgtcgcgggt caaaaagctg gtgccaatga
    14101 tcatcaggtt gggcagaaag acaaacaaca caagccaacc gacaatagtg acaatcacta
    14161 cattctggaa cttacttgtg ttcttcatca gccagtacga cctcccagct ttctacccaa
    14221 ttaatggcca ttttttggtc gagagagtgg tcaaagtcag gatcgtcttc attgaagaat
    14281 tcgctgacca tcaccatctt gccattttcc agttcaacaa ccgactccag cgtcatgcct
    14341 ttgtagttac gctcgcgaac gtaaccaatc agcccttcag cgtggttgtc gtcgttaatc
    14401 tcttcaacac gtaagtcttc cgggcgcagc agaacatgca gtttttgccc cggttcaacg
    14461 gcgaagttaa cgtagatatt acattcgcgg ccttcaacgt tggcgcgtac gcgctgctcg
    14521 tcgagacgtt cgatgacagt ggcgttaaac atattgattt cgccaatgaa cccggcaaca
    14581 aacaggtttt tcggctcttc gtagatttca cgcggcgtgc cgtcttgctc aatgcgacca
    14641 tcgcgcatca ccacaatcct gtccgacatg gtgagtgctt cttcctggtc gtgagtcacg
    14701 aagacgaatg taatgccaag cttacgctgt aacgctttca gctcgttctg catttgcttg
    14761 cgcagtttgt aatccagcgc tgagagcgac tcatccagca acaacagacg aggcttgtta
    14821 accaccgcgc gagcaatggc gacgcgttgc tgttgaccac cagagagctg atgcggttta
    14881 cgttgagcga aggtttccag ctgcaccatc cgcagggctt ccatcacgcg gggcgtaatt
    14941 tcagcagcgg gggttttttg catgcgcaac ccaaaggcca cattttcgaa cacggtcatg
    15001 tgggggaaaa gtgcgtagct ttggaaaaca gtgttcacat agcggttttc cgccggaacg
    15061 tgggtgatgt cctcgttatc caacatgatg cgtccggaat caacagtttc cagacctgca
    15121 atcaggcgaa gaacggttgt tttaccgcag ccagaagggc caagcagcgt gaggaactcg
    15181 ccattgttga tagtcagatc cagctgggga atgacctctt taccatcaaa gcatttgcga
    15241 attcccgcca attgcaccag cggtgaaagc gaactcggtt gtttattcaa ttttttactc
    15301 tgtcccatgt aaacgcaacg gatggcttac cgatgcgggg tttgtggtta accaccttgg
    15361 tgactcttaa tgagggcggt aattctacgg caaaccgctt gaatcgccaa tctttgttgt
    15421 gaattactgg cttagcttta tattcattaa ggtaatgctg ataaatattc ccgcttgcag
    15481 gggtaaaagt gacctgacgc aatatttgtc ttttcttgct tcttaataat gttgtcacaa
    15541 aaagtgaggg tgactacatg gataaactac ttgagcgatt tttgaactac gtgtctctgg
    15601 atacccaatc aaaagcaggg gtgagacagg ttcccagcac ggaaggccaa tggaagttat
    15661 tgcatctgct gaaagagcag ctcgaagaga tgggccttat caatgtgacc ttaagtgaga
    15721 agggcacttt gatggcgacg ttaccggcta acgtccctgg cgatatcccg gcgattggct
    15781 ttatttctca tgtggatacc tcaccggatt gcagcggcaa aaatgtgaat ccgcaaattg
    15841 ttgaaaacta tcgcggtggc gatattgcgc tgggtatcgg cgatgaagtt ttatcaccgg
    15901 ttatgttccc ggtgctgcat cagctactgg gtcagacgct gattaccacc gatggtaaaa
    15961 ccttgttagg tgccgatgac aaagcaggta ttgcagaaat catgaccgcg ctggcggtat
    16021 tgcaacagaa aaacattccg catggtgata ttcgcgtcgc ctttaccccg gatgaagaag
    16081 tgggcaaagg ggcgaaacat tttgatgttg atgccttcga tgcccgctgg gcttacactg
    16141 ttgatggtgg tggcgtaggc gaactggagt ttgaaaactt caacgccgcg tcggtcaata
    16201 tcaaaattgt cggtaacaat gttcacccgg gcacggcgaa aggagtgatg gtaaatgcgc
    16261 tgtcgctggc ggcacgtatt catgcggaag ttccggcgga tgaaagcccg gaaatgacag
    16321 aaggctatga aggtttctat cacctggcga gcatgaaagg caccgttgaa cgggccgata
    16381 tgcactacat catccgtgat ttcgaccgta aacagtttga agcgcgtaaa cgtaaaatga
    16441 tggagatcgc caaaaaagtg ggcaaagggt tacatcctga ttgctacatt gaattggtga
    16501 ttgaagacag ttactacaat atgcgcgaga aagtggttga gcatccgcat attctcgata
    16561 tcgcccagca ggcgatgcgt gactgcgata ttgaaccgga actgaaaccg atccgcggcg
    16621 gtaccgacgg cgcgcagttg tcgtttatgg gattaccgtg cccgaacctg ttcactggcg
    16681 gttacaacta tcatggtaag catgagtttg tgactctgga aggtatggaa aaagcggtgc
    16741 aggtgatcgt ccgtattgcc gagttaacgg cgcaacggaa gtaagcgaaa agggatgcgg
    16801 catgtgatgc cgcatccggc ttaaatccaa acttaccctt cgaagaacca atacccgcta
    16861 ttgaccagcg ccgcgagcat cgcgaggaat gacggatctt ccagcgcatc gccaaaattc
    16921 tccgcggtca gcgcaatgtt gctggcgagt gcatccagtg ccggacggtg cggggaatcg
    16981 atcttctcac cattggcata cacgtcgtcg ccaatgcgca atacgcgcag accacccagg
    17041 cgcaccagca catcaccttg tttcagcgca tcgtagattt catccggctg ataaggcggc
    17101 tctggcggcg cgatatccag ttcatgacgt gactgggata taaactcccc aaaccattgc
    17161 ttaaagtgtt ccggctggtt gatcaattcg agcatcatct cacgcagttt atccatctct
    17221 tgcggcagaa catccgcagg atgagcgcga ggtggaacat caggatcgct gtagtagttg
    17281 ccgcccagtt cacgttgcag cacataatcg gcaaatccac taatcagttc ccgcgtattt
    17341 ggcgcgcgaa aacccacgga atagttcatc gcattttcca gcgcgtagcc ttcatgcggg
    17401 aatcctggcg gaatataaag aatatcgcca ggctccagct cttcatcgat gatggcttcg
    17461 aacggatcga cctgtaacag atccgggtgc ggacagtgct gtttcatttg cagcttttcg
    17521 cccactcgcc agcgacgacg accggtaccc tgaatgataa acacgtcgta ctgatcgaga
    17581 tgcgggccga cgccgccgcc aggtacagaa aaagagatca tcagatcatc aatacgccag
    17641 tccggtaatt cacggaacgg tcgcatcagc gcggcggtcg gctcatgcca gtggttcact
    17701 gcttgcacta acaatgacca gttagtttca ccgagatgat cgtagctttc gaacggaccg
    17761 tggctgacct gccattttcc atcctggtga ctgaccagtc gactgtcgac ttcgctttcc
    17821 atcgccagac ccgccaactc gtctggagag atcgggtcaa taaaattatt aaagccgcgt
    17881 tttaacacca ccgggcgttt ctgccagtga cgttcaagaa aatcgggcca gttaagagtg
    17941 agttggtatt ccatgttaag catccacagg ctggtatctg caaccgatta taacggatgc
    18001 ttaacgtaat gcgtgaagta tggacatatt tattcatctt tcggcgtaga atgctggcga
    18061 ccaaaaatca cctccatccg cgcaccgcca agcatgctct ctccggcgac gattttaccc
    18121 tcatattgct cggtgatttc gcgggctacc gccagcccca caccttgccc agggcgtaaa
    18181 gtatcaaccc gttgaccacg gtcgaaaatg acctctcgct tgcttaatgg aataccgggt
    18241 ccatcatcct cgaccacaat atagagatgc tcgtcggttt gccttgcaga aatttcgaca
    18301 aactcgaggc aatatttaca ggcattatcc agcacattgc ccatcacctc gacaaaatcg
    18361 ttctgctcac cgacaaagct gatctctggc gaaatatcga gagagatatt gacccctttg
    18421 cgttgataca ctttgttcag cgccgaggtg agattgtcca gcagtggggc gaccggatgc
    18481 agctcgcggc tgagcaatgt cccgccgcgc atactggcac gatgcaggta gtagccaatt
    18541 tgctgtgaaa tgcggctgat ttgctccagc attaccggct cagcatcact gacgctcatt
    18601 ttctcactac gcagagaacg cagcgtactt tgcagcaccg ccagtggcgt tttcagacta
    18661 tgggtcaggt cggtgagcgt tgtacgatat ttgtcgtaac gttcgcgttc actttttaac
    18721 aatcggttca ggtttcgtac cagactggtc agttctcgcg ttgtggctgg attgagcaat
    18781 tcgcggttat gttcttccag ttcgcggact tcttttgcca gggcttcgat ggggcgtaaa
    18841 ctccaccagg cggcgaccca cagcagcggg atcactaaca gcagattggc tgagagcaca
    18901 tagataaacc agctccagac catataggaa ctttttagct ccaccggaat ggtatccacc
    18961 accacaatgg ttaactttgg catccgcgat gttgccgggt agacgtttac cgccaccgag
    19021 tgggtcatct ccgcgtcgtc atcatcttcc cgcacttcct gcaactgttg ctgtatcgaa
    19081 tgatctccac tcagcaagag gctggtatcg ttaacatccg cttcaatttc atgaaaacca
    19141 ttcgatttca gccagtcagg ctggatcatc ttcatcagcc agggcacgtc acgttgcgcc
    19201 cataaaagct gcccgttctc atcataaatt agcgtcatgg tggggctttg cttgtcgata
    19261 ttttcgggta actcgacatg caacttattg ttttcccact tcgcaagggt atagaacaga
    19321 ttgctctcgc cacgtaacag ccgaaacgta gttttatcga aactgacgct ataaccgatc
    19381 agcgcgacca ttccgtaggc aagcgaaagc accagtacta ccgctgccgt tgccaacaga
    19441 aaacgtaccc gcagcgagag cgggaaaaaa agacgcagta attttttcat cagcgcaatt
    19501 cgaacagata gccctggccg cgaacggtgg taatcacttc ttggggatat tgtgcctgaa
    19561 tttttttgcg cagacgtccc atcagtacat caatggtatg gctttcccgc agctccgcat
    19621 ccggatagag ttggagcatt aacgaatctt tgctcaccac tttgccatta ttgcgtatca
    19681 acgtttccat aatggtgtat tcgaacgcgg tcagtttgat cacttcgtca ttaatagata
    19741 attcacggcg agagagatca acctgaaacg gtgggagcga aatgacctgt gaagccaggc
    19801 cgctattacg ccgcattaat gcctgcattc gcgccatcac ctcttcaata tgaaacggtt
    19861 tagtcacgta atcatcagca ccggcactta atacttcgac tttgtcctgc cagctttcac
    19921 gggcggttaa taccagaatc ggcagtgaaa catcattgct acgccagcgg cgaatcagtg
    19981 acagaccgtc ctcgtctggc aatccgagat cgacaatcgc aatatccggt aaatgttcat
    20041 tgagataata atcggcttct ttggcatctt ctgcatcatc gacctgatga ccagcatcct
    20101 gaatctgaac tttaaggtgg tgacgtaaca acgcattgtc ttcaacaacc agtacgcgca
    20161 tttttatttc tccctgtctt aattattaaa atagtgtaac gcgattatgt ggttatgggg
    20221 gtaaacatta aataaaccag cggggagggg aggtaaagtg aaaaaataaa aagcggataa
    20281 tcttaataag caggccggac agcatcgcca tccggcactg atacgagttt atttcagctc
    20341 atcaaccatg gtgatggcgc gaccaatgta gtttgccggc gtcatcgctt tcaggcgggc
    20401 tttctcttct tctggcaacg ccagaccgtc gataaactgc ttcatgcctt cggcgtcaac
    20461 gcgcttacca cgagtcagct ctttcaattt ctcgtacggt ttttcgatgc catagcgacg
    20521 cataactgtc tggattggtt ctgccagcac ttcccagttg tgatccagct catccagcag
    20581 atggtcacgg ttcacttcca gtttgctcac gcctttcagg gtggattgat acgcaatcag
    20641 cgcataaccg atacccacgc cgaggttacg caacacggtg gagtcggtca ggtcacgctg
    20701 ccagcgggaa accggcagtt tgcttgccag atgctgtaat accgcgttgg aaaggcccag
    20761 attcccttcg gagttctcga agtcgatcgg gttaacttta tgcggcatgg tggaagaacc
    20821 aatctcaccg gcgatggttt tctgtttgaa gtggttaagg gcgatataac cccagacgtc
    20881 acggtcaaag tcgatcagaa tggtgttgaa gcgcgcaacg caatcaaaca gttcggcaat
    20941 gtagtcgtgc ggttcaatct gggtagtgta cgggttccat tgaataccca gcgaggtgac
    21001 gaactcttcg ctgaactgat gccagtcaac ttccgggtaa gcggcgatgt gggcgttata
    21061 gttaccgacc gcgccgttga ttttgccgag gatctccacc tggttaagct ggcggtactg
    21121 gcgctccata cggtaggcga cgtttgccat ctctttaccg atggttgacg gcgtggctgg
    21181 ctgaccgtgg gtacgagaca gcagcgggat atcgcggtac tgaacggcga gatctttaat
    21241 gccatcaatt agttgacgcc agtacggcag gatcacttca tcacgcgcgg ttttcagcat
    21301 taatgcgtgg gagaggttat tgatatcttc cgaagtacag gcaaagtgga tgaattcaga
    21361 aaccgcgtgc agttccggga tctccgccac tttttctttc aggaaatact caaccgcttt
    21421 aacgtcgtgg ttagtagtac gctcgatggt tttgatgcgt gcggcatctt cttcgctgaa
    21481 attggcgaca attgcatcaa ggtaaccgat tgcgtcggca gcaaaagcag gaacttcctt
    21541 gatcgctgcg tgcgcggcca gtttttgcag ccaacgtact tcaacttgta cacggaattt
    21601 cagcaaacca tattcgctga aaatcccgcg cagcgcgctg actttatcgc cgtagcgtcc
    21661 atcgacaggg gaaacggcgg tcagtgagga taattccata gatcacaact ccggggttaa
    21721 atgagcaaga atttgttttg cctgagtggt caggcgatta cgagaaaaca tcagttgcag
    21781 acgtccgccg ccgacctggt gccagagcac ggcggcgcga atgcctgcca gcagggttgc
    21841 gcgaactttc gcctgcactt gtgggctttg cagtacagca ggggagccgg tgacctgaat
    21901 gcgcgggcca agcgggctaa tcacatcaac atagatagca gccatcgcgc tcatcagcgt
    21961 ttcggactgt aaatcgaagt gttcgagctg gcgttgcagg ccgttgatcc ggttgcccag
    22021 agtgtcgagc gcacctttcg ctgaggagag tttgcgctca agcaccatca ggctgagtgt
    22081 gtagcgggtt aattcggcgt ttaagccctg gcgactgctg gcattgagca cgccgagcag
    22141 ggtttccagc ccgacgcgca ggttggcttc gctaccgcca aaaaccgcca gcgtcgagct
    22201 ggggttcata tcaataatac tgttgagtga gacgtgtagc gcatcggcat cacaatgccc
    22261 ctggtgagcg agttgttgca ccaggcgtgc cgactgacaa ataccggcca gggcgagggt
    22321 gatgtcatag taattctttg ccacgttcac tgcttccttg ttttaagtaa agataataat
    22381 cagaccggca gcggcagacg ctgctcaata ataccgccac cgaggcacac ttcaccgtta
    22441 tagaagacgg cagactggcc cggcgtcacg gcggcaacgg gctcatcgaa aatcacttca
    22501 atgcgatcat cgtccagcgc cttgacggtg caggggatat cggtctggcg atagcgggtt
    22561 tttaccgtgc aacgcatagt accggtgaat ggttcacgat cgacccagtg cagctgctgg
    22621 gcaatcagac cgacagacat cagacgcggg tgttcatggc cctgcgcaac aatcagaatg
    22681 ttgttttcga cgtctttgtc caccacatac cacggttctt cggtgccttc tttggtgcca
    22741 ccgataccca gacctttacg ctgaccgagg gtgtgataca tcagcccctg gtgctcacca
    22801 atttcatcgc cgtcaacagt aatgattttg cccggttgtg ccgggagata acggcccagg
    22861 aactcgcgga atttacgttc gccaatgaag caaatgccgg tagagtcttt tttcttcgcg
    22921 gtgaccagac caagatcttc agcaatctta cgcacctgcg gtttttccag ttcgccgacc
    22981 gggaacaggc tttgcgcaat ctgctcatgg ctgagcgtat aaaggaagta gctctggtct
    23041 ttattgctgt ccagaccacg cagcaggcgg ctcttgccat cgacatcgac ccgacgaacg
    23101 taatgaccgg tagcgatata atcggcacct aaatcttcgg cggcaaattc gaggaaggct
    23161 ttaaatttga tctctttgtt gcagagaata tccggattcg gcgtgcgacc ggctttatat
    23221 tcggcaagga acagttcgaa gacgttgtcc cagtactcgg cagcaaagtt aacggtgtgc
    23281 agttcaatgc cgagcttgtc gcagacagcc tgggcatcag ccagatccgc tgccgctgtg
    23341 caatattcct caccgtcgtc ttcttcccag ttcttcataa acaggccttc gacctgatat
    23401 ccctgttgtt gcaacagcca ggcagaaacg gaggaatcaa caccgccgga catgccgacg
    23461 attacttttt ttgcggtttc agacattgga tcactcacga cattgaactt caaggcggcg
    23521 tattctatca cgcagccttt tagatgacac cctttgtaaa aggccagtta aaatcgccaa
    23581 tcatctccag cggatagcgt tgcccgcttt gataacaacg tatactttcc gccaccagtg
    23641 gcgaacgaag atttgacgcc tgtaaaattt cttcggcgct aacccaacgg cagcagtcga
    23701 tatcgctgtc atgaggctgg gtcgggcata tttgctcaag ctcaatggca aaaaggaaac
    23761 gcaaaaacgg cgttttatct ggcgcaatcc actgatgcat acgaataaag tgttgcggct
    23821 gcgcgctgat gccggtttct tcccacagct cacgggcggc ggcttccact aaggtttcat
    23881 cggcctccag atgcccggca ggttggttcc ataaggcttt accattaatc gtctcttcaa
    23941 cgactaaaaa tttgccttct gcgtgcacca cgcaagcaac ggtaacgtgc ggtttaaaca
    24001 ttttgcatcc ttaatctgtc acttctcgcc attcgccatt agcaagatta tccaaagagt
    24061 aatcacccat cgcatagcga atcagtcgca gcgtggggaa accaacatgg gcggtcatgc
    24121 ggcgcacctg gcgattacgt ccttcatata aggtgatctt cagccagctg gtgggaatac
    24181 ttttgcgttc acgaattggt ggattccgcg gccataacca cgcaggttcg tcaaccagct
    24241 ccgcgccggc gggcagggta gggccatcat ttaaggttac gccattgcgc aaggcttcaa
    24301 gtgcgtcttg tgtgggaatg ccttccacct gcacataata gatttttccg gtgcgtttgc
    24361 ctggctgggt taaacgcgcc tgtagtgcac cattattggt cagcaccaat aacccttcgc
    24421 tatcgcggtc aagacgacct gctgcataaa cgccctgaac cgggatgaat tcttttaatg
    24481 ttttgcgtcc ggcttcatcg gtgaactgcg gaagaacatc gtagggttta ttgaacagga
    24541 tcacacgcgt gggctggttt tcaggtttac gcctggtaga acgttgcgag ctgaatcgct
    24601 taacctggtg atttctaaaa gaagtttttt gcatggtatt ttcagagatt atgaattgcc
    24661 gcattatagc ctaataacgc gcatctttca tgacggcaaa caatagggta gtattgacaa
    24721 gccaattaca aatcattaac aaaaaattgc tctaaagcat ccgtatcgca ggacgcaaac
    24781 gcatatgcaa cgtggtggca gacgagcaaa ccagtagcgc tcgaaggaga ggtgaatgga
    24841 aagtaaagta gttgttccgg cacaaggcaa gaagatcacc ctgcaaaacg gcaaactcaa
    24901 cgttcctgaa aatccgatta tcccttacat tgaaggtgat ggaatcggtg tagacgtaac
    24961 cccagccatg ctgaaagtgg tcgacgctgc agtcgagaaa gcctataaag gtgagcgtaa
    25021 aatctcctgg atggaaattt acaccggtga aaaatccaca caggtttatg gtcaggacgt
    25081 ctggttgcct gctgaaaccc ttgatctgat tcgtgaatat cgcgttgcta ttaaaggccc
    25141 gctgaccact ccggttggtg gcggtattcg ttctctcaac gttgccctgc gccaggaact
    25201 ggatctctac atctgcctgc gtccggtacg ttactatcag ggcactccaa gcccggttaa
    25261 acaccctgaa ctgaccgata tggttatctt ccgtgaaaac tcggaagaca tttatgcggg
    25321 tatcgaatgg aaagctgact ctgccgacgc cgagaaagtg attaaattcc tgcgtgaaga
    25381 gatgggcgtg aagaaaattc gcttcccgga acattgcggt atcggtatta agccgtgttc
    25441 tgaagaaggc accaaacgtc tggttcgtgc agcgatcgaa tacgcaattg ctaacgatcg
    25501 tgactctgtg accctggtgc acaaaggcaa catcatgaag ttcaccgaag gcgcgtttaa
    25561 agactggggc taccagctgg cgcgtgaaga atttggcggt gaactgatcg acggcggccc
    25621 gtggctgaaa gttaaaaacc cgaataccgg caaagagatc gtcattaaag acgtgattgc
    25681 tgatgcattc ctgcaacaga tcctgctgcg tccggctgaa tatgatgtta tcgcctgtat
    25741 gaacctgaac ggtgactaca tttctgacgc cctggcagcg caggttggcg gtatcggtat
    25801 cgcccctggt gcaaacatcg gtgacgaatg cgccctgttt gaagccaccc acggtactgc
    25861 gccgaaatat gccggtcagg acaaagtaaa cccaggctct attatactct ctgctgagat
    25921 gatgttgcgc cacatgggtt ggaccgaagc cgcagacctg attgttaaag gtatggaagg
    25981 cgcaatcaat gccaagaccg taacctatga cttcgaacgt ctgatggaag gcgctaaact
    26041 gctgaaatgt tcagagtttg gtgatgcgat catcaagaac atgtaatctc tacatgtgtt
    26101 aaatattgaa acgggcgtat aacacgcccg ttgttttatt tatgtggata ttattaatag
    26161 catatcgagc atatttatat aaaacctatt acttgagccc atatgggcat atttttataa
    26221 tacaactctt atgcaaacat ttatttgtta tttcgctttc tcctggagga cactcttgac
    26281 tgcttttaag taaactccat aaattcttgt tgaatggtgc aatgttataa atagtaatag
    26341 gatattcttt atccttaagg ataataccag gcttaaccgg tgtaaatata cttccaggag
    26401 gaagaaatat agtagattga taccagatga tcattttcat atcaccccat atggctgaaa
    26461 aagatatgcc gcatgtaggt tgaattaccg tgtcaattac tacccacttc atttgttatg
    26521 tcttatccca cagtatt
//
LOCUS       DAZGCQ010000067        26344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000067 DAZGCQ010000000
VERSION     DAZGCQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.067"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            312..4283
                     /gene="ehaA"
                     /locus_tag="ACHK07_003087"
     CDS             312..4283
                     /gene="ehaA"
                     /locus_tag="ACHK07_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO8103385.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LALSKDVAVSEDYTAVFGKDNQTIDYRISVTDNANLVIDATDSSRPRLTLASGGGLDI
                     TGGKVHINGSLNFLLKGTGFLNVANAGSELYADDLYESYSNITHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     VPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDIAQWSHNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
     gene            4425..5513
                     /gene="pdeL"
                     /locus_tag="ACHK07_003088"
     CDS             4425..5513
                     /gene="pdeL"
                     /locus_tag="ACHK07_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8103386.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(5555..6487)
                     /gene="yahB"
                     /locus_tag="ACHK07_003089"
     CDS             complement(5555..6487)
                     /gene="yahB"
                     /locus_tag="ACHK07_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8103387.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(6579..7076)
                     /gene="yahC"
                     /locus_tag="ACHK07_003090"
     CDS             complement(6579..7076)
                     /gene="yahC"
                     /locus_tag="ACHK07_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8103388.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLCQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            7334..7939
                     /gene="yahD"
                     /locus_tag="ACHK07_003091"
     CDS             7334..7939
                     /gene="yahD"
                     /locus_tag="ACHK07_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO8103389.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQVLIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7979..8842
                     /gene="yahE"
                     /locus_tag="ACHK07_003092"
     CDS             7979..8842
                     /gene="yahE"
                     /locus_tag="ACHK07_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8103390.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     HDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRFRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8832..10379
                     /gene="fdrA"
                     /locus_tag="ACHK07_003093"
     CDS             8832..10379
                     /gene="fdrA"
                     /locus_tag="ACHK07_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8103391.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIDAI
                     KEAKAIAAAGGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10379..11797
                     /gene="yahG"
                     /locus_tag="ACHK07_003094"
     CDS             10379..11797
                     /gene="yahG"
                     /locus_tag="ACHK07_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8103392.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFEQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11823..12077
                     /locus_tag="ACHK07_003095"
                     /pseudo
     CDS             11823..12077
                     /locus_tag="ACHK07_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12127..13077
                     /gene="yahI"
                     /locus_tag="ACHK07_003096"
     CDS             12127..13077
                     /gene="yahI"
                     /locus_tag="ACHK07_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO8103393.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTEVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STVLLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPESLPAALRGETGTHIIKT"
     gene            13087..14469
                     /gene="yahJ"
                     /locus_tag="ACHK07_003097"
     CDS             13087..14469
                     /gene="yahJ"
                     /locus_tag="ACHK07_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO8103394.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            14682..14759
                     /locus_tag="ACHK07_003098"
                     /pseudo
     CDS             14682..14759
                     /locus_tag="ACHK07_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14846..15895
                     /gene="yahK"
                     /locus_tag="ACHK07_003099"
     CDS             14846..15895
                     /gene="yahK"
                     /locus_tag="ACHK07_003099"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO8103395.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            16138..16953
                     /gene="yahL"
                     /locus_tag="ACHK07_003100"
     CDS             16138..16953
                     /gene="yahL"
                     /locus_tag="ACHK07_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO8103396.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQVLAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            17368..17613
                     /gene="yahM"
                     /locus_tag="ACHK07_003101"
     CDS             17368..17613
                     /gene="yahM"
                     /locus_tag="ACHK07_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO8103397.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAWIRQIRLIT"
     gene            complement(17630..18301)
                     /gene="yahN"
                     /locus_tag="ACHK07_003102"
     CDS             complement(17630..18301)
                     /gene="yahN"
                     /locus_tag="ACHK07_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO8103398.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            18448..18723
                     /gene="yahO"
                     /locus_tag="ACHK07_003103"
     CDS             18448..18723
                     /gene="yahO"
                     /locus_tag="ACHK07_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO8103399.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(18824..20410)
                     /gene="prpR"
                     /locus_tag="ACHK07_003104"
     CDS             complement(18824..20410)
                     /gene="prpR"
                     /locus_tag="ACHK07_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO8103400.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            20649..21539
                     /gene="prpB"
                     /locus_tag="ACHK07_003105"
     CDS             20649..21539
                     /gene="prpB"
                     /locus_tag="ACHK07_003105"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO8103401.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQVK"
     gene            21699..22868
                     /gene="prpC"
                     /locus_tag="ACHK07_003106"
     CDS             21699..22868
                     /gene="prpC"
                     /locus_tag="ACHK07_003106"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO8103402.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSAIGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            22902..24353
                     /gene="prpD"
                     /locus_tag="ACHK07_003107"
     CDS             22902..24353
                     /gene="prpD"
                     /locus_tag="ACHK07_003107"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO8103403.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAVTLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            24393..26279
                     /gene="prpE"
                     /locus_tag="ACHK07_003108"
     CDS             24393..26279
                     /gene="prpE"
                     /locus_tag="ACHK07_003108"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO8103404.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         6706 a         6431 c         7129 g         6078 t
ORIGIN      
        1 atattaaatg tatttctcct gcttttgttt atattttgag ttgatttgag ttcccggata
       61 catataatgc gcaggtatta gagactgggg ggggaatgta atgataaagt taagagtgtt
      121 aacttaaaat aagttttcag tagtaacttg ctattttatt accctctgaa ctacatactc
      181 agatatgtgg ttgccaatgc tatgcaaaat ttttgaattt ttatatgcga tgaacagtaa
      241 ccggaatggc atttaatgcg ttgctgttta tgcaatcttg gttttatttg gatgtattgc
      301 tggagattgt gatgaataaa atatatcggc taaagtggaa caggtcccgt aactgttgga
      361 gcgtctgctc tgagctgggg agcagagtaa aaggaaaaaa gtcccgggct gttttaatta
      421 gcgcgataag tttatattca tctctggcac tgtctaaaga tgtcgctgta agtgaagatt
      481 acacagctgt attcggtaaa gataaccaga ctattgatta ccgtatttca gtgaccgaca
      541 atgccaatct ggtaatcgat gcgacagatt cttctcgtcc acgtctgact ctcgcgtctg
      601 gtggtgggct ggatatcact ggagggaagg tgcatatcaa tggctcgctt aactttttat
      661 taaaagggac ggggtttctg aacgtcgcca atgctggcag cgagttatat gctgatgatt
      721 tatatgagtc ttactctaat attacccacg atagaggcta ttttaatgtc tccaacggcg
      781 gcaaaatcca tgttaagggc accagccgtc tgacctattt gcagggaaat gtcagcggtg
      841 aaggcagcca ggtaaattct aaaaccttct ttatgggcgt ttacggcagt tacggtggta
      901 atcagtacct gtcagttaat aacggcggtg aagttaatgc cagtgagaaa attagcctgg
      961 gttattatga tcaaaactcc gatacaacac ttgccgtttc ggaaggtggt aaaatctctg
     1021 cgccgactat tagtttaagc accaactctg agttagcgtt aggtgcacag gaaggaagcg
     1081 cagcgaaggc tgcagggatt attgatgccg aaaaaattga gtttgtgtgg gcaaagacat
     1141 ccgagaagaa aatcacctta aaccacacgg ataaaaacgc gactatttcc gcggatattg
     1201 tcagtgacag cgagggcctg ggctatatca atgcgctcaa tggtacgact tacttaaccg
     1261 gggataactc cgcctttagc ggtaaagtca aaattgagca aaatggcgct ttagggatca
     1321 cccaaaatat aggtacagca gaaatcaaca accgcgggaa attacacctg aaggctgacg
     1381 atagcatgac ctttgccaat aagatttccg gcaatggtac aataagtatc gacagtggga
     1441 cagtggcact gaccggcaat aactatgcat tcagcggata tattgatgtc gcttctggtg
     1501 ctgtcgcggt tatttctgaa gacaagaaca tcggccgtgc ggacctggat gttgatggca
     1561 aactgcaaat caatgccaat aaagattggg tatttgataa cgatcttgaa ggtagaggta
     1621 ttgttgaaat aaacatgggg aatcacgaat tctccttcga tgagtttgct tatacagact
     1681 ggttccaggg ttcactggcg ttccagaaca cgacatttaa tctggaaaag aatgctgagt
     1741 ttctgcagaa aggcgggatc actgcgggtc agggaagcct ggtaacagtg ggtaagggcg
     1801 ctcactccat tagcactttg ggattctccg gcggaaccgt tgattttggt gccctgacag
     1861 caggtgcaca gatgacagaa gggacggtca acgttagtaa aacgctggat ttgcgcggcg
     1921 agggtgtgat tcaggtttct gacagtgacg ttgtaagctc agtatctcgt gatattgact
     1981 ctgcgttatc gctcactgaa gtcgatgatg gtaacagcac cattaagttg gttgatgcgc
     2041 aaggtgcgga agttctgggc gatgcgggca atctgcaatt gcaggataaa aatgggcaaa
     2101 tcctctccag cagcgcccaa cgtgatattc agcagaatgg gcaaaaagcg gccgtcggca
     2161 cttacgacta tcgtctgacg agtggggtaa acaatgacgg tctgtatatc ggttacggcc
     2221 tgacccagct tgatttacac gctaccgaca gcgatgctct ggtgctgagc tctaacggta
     2281 aaagcgagaa tgccgccgat ctcagcgcaa agattaccgg cagtggtgac ctggcattca
     2341 gcagccagaa gggtcagacc gtatcgcttt ctaacaaaga caacgactat accggtgtta
     2401 ccgatctgcg cagtgggacg cttttgttga ataacgataa cgtgttgggt aatacccatg
     2461 aactgcgtct ggcggcagag actgaactgg atatgaatgg tcacagccag accgtgggca
     2521 cgctcaatgg cagcgccgat tcactgctga gcttaaatgg cggcagtctg acggttacca
     2581 acgggggcac ttcaaccggt tcgttaacgg ggagcggaga gctgaatatt cagggcggca
     2641 cgctggacat cgcgggcgat aacagcaacc tgacggcgaa tgtgaacatt gctaattcgg
     2701 ctaatgtcct ggtaagtcat gcgcagggat tgggtagcgc aaacgttgag aacaacggta
     2761 ccctggcgtt gaataatagc gctgaaaaaa gagcggctgc gtctgtgaat tacaccctgg
     2821 gcggcaatct gaccaacaac ggtacgctga tgaccggaat gtcaggacag caagctggca
     2881 atgtgttagt ggtgaagggg aactaccacg gtaataacgg tcaactagta atgaatacgg
     2941 tactgaatgg cgatgactca gtaaccgata aattggttgt cgagggcgat actagcggca
     3001 cgactgccgt tacggtgaat aacgctggcg gtacaggtgc gaaaaccctt aacggtatcg
     3061 aacttatcca tgtagacggt aagtctgagg gcgaatttgt tcaggctggt cgtatcgttg
     3121 cgggggcgta tgactacact ctcgcgcgtg gacaaggggc aaatagtggt aactggtatc
     3181 tgaccagcgg cagtgattct cctgaactgc agccggagcc agacccggtg ccgaatccga
     3241 atccagagcc gaaccctaac ccgacaccta cgccgggccc ggatctgaat gtggataatg
     3301 acctgcgacc ggaggcgggt agctacattg cgaaccttgc agcagcgaat accatgttca
     3361 ccacgcgtct gcatgagcgt ctgggtaata cgtactatac cgacatggtg acgggtgagc
     3421 agaaacaaac cactatgtgg atgcgccatg aaggtggtca taataaatgg cgtgatggca
     3481 gcggccagct gaaaacccaa agcaatcgct atgttctgca actgggaggc gatatcgcgc
     3541 agtggagcca taacggcagc gacagctggc atgttggggt catggcggga tatggcaaca
     3601 gcgacagcaa aaccatttcc tcgcgaaccg gttatcgtgc aaaagcgagt gtgaacggat
     3661 atagcacagg cctctatgcc acctggtatg ccgatgacga gtcgcgtaat ggcgcgtatc
     3721 tcgacagttg ggcgcagtac agctggtttg ataacacagt gaaaggggat gacttacaaa
     3781 gtgaatccta taaatcaaaa ggatttaccg cttcactgga agctggatac aaacacaaat
     3841 tagctgaatt taatggcagc cagggaacgc gtaatgaatg gtatgttcag ccgcaagcac
     3901 aggttacctg gatgggagtc aaagccgata agcaccgcga aagcaacgga accctcgttc
     3961 atagcaacgg tgatggcaat gttcaaaccc gacttggcgt aaaaacctgg ctgaagagcc
     4021 accataaaat ggatgacggt aaatcccgcg agttccagcc gtttgtagaa gtgaactggc
     4081 tgcataacag taaggatttc agcaccagta tggatggcgt gtccgtcact caggatggag
     4141 cccgaaatat tgctgagata aaaaccgggg tggaaggaca gctaaatgcc aacctgaatg
     4201 tctgggggaa tgtgggcgtt caggttgccg ataggggata taatgacacc tctgcaatgg
     4261 ttggcattaa gtggcaattc tgatctgaat aatgagtaaa aggagagaga gccgaacggg
     4321 agataattcg gctctctgtg tcattttctt gcagccagct ttatccgtca gagtgaagac
     4381 tcgcagattt agctggcgca caagatttaa tcaaggtgtg ttcaatggct tcgcatgatt
     4441 tgagtgtttt tttggaagag ttcggggcaa cggtgaattt gacgttgcct ggtatcgtgt
     4501 ccgaaaaaga acggctttta ctcaagctgc taatggaggg aatgtcggtt acagaaatct
     4561 cacagtacag aaatcgcagt gcaaagacaa tttcacatca aaagaagcag ctgtatgaaa
     4621 aactaggcat ccagagtgat attaccttct ggcgggatat cttttttcaa tatcatccgc
     4681 aagtcatcag tggcacggga aataaaaata atttttatat ccctgataac cgttgtcacc
     4741 atatcgtcac gcctgaagcc atcagtctgg cgctggaaaa ccacgaattc aaaccgtgga
     4801 tccaaccggt tttctgcgcg cagactgggg tactgacggg ctgtgaggtg cttgtccgct
     4861 gggaacatcc acaaacggga atcatcccgc cggatcagtt tattcctctg gcggagtcat
     4921 ccggtcttat tgtcataatg acccgccaac tgatgaaaca gactgcggat attctgatgc
     4981 cggtaaaaca tttgctgccg gataatttcc atattggtat caacgtctcg gcgggttgtt
     5041 ttttggccgc gggatttgaa aaagaatgcc tgaacctggt caagaaatta ggaaacgata
     5101 aaatcaaact ggttctcgag ctaacggaac gtaaccctat tccggtaacg ccagaagcca
     5161 gagcgatatt tgacagcctt catcagcata acattacctt tgcgctggat gactttggta
     5221 cgggatatgc gacctatcgt tatctgcagg cgtttccggt cgattttatt aagatcgata
     5281 agtcatttgt gcaaatggcg agtgtcgacg aaatatccgg tcatattgtg gacaatattg
     5341 tcgaactggc gcgtaagcct ggtctgagta tcgtggcgga aggggtagaa acccaggagc
     5401 aggcggattt aatgatcggt aaaggcgttc actttttgca gggctatttg tactctccgc
     5461 cagtaccggg taataaattt gtttctgaat gggtaatgaa agctggtggt tgatgtaaac
     5521 cgctattcac agcgcatcgg gaggttggca gcgattaatt ctcccgatgc agtaaatcct
     5581 gatagatccc cgttaatatg ccatttggcg caaattgctt tttaatccac tgcgtcacct
     5641 gacccgtagc agaatgctga gtcgccagta acatgcgcga atcctggcgc ggattatgga
     5701 tttgtcgggt aaccagcagg gattgcgtca tcgcctcacg gaccatgtaa tccggcaaaa
     5761 aaccaatccc ttcacctaaa atctggcact gacatttggt gttgaaatct ggcaccagaa
     5821 ttgactcctg cccgtgcagc aaccagccca cttttttatt aatcgtatgc gcggtgtcct
     5881 ccaccataat attagggtag agacgcagtt ggctttcggc gatgggttcc ggcacaaagg
     5941 ctaacgggtg atccggggcg atagcaaaag cccagcgaat cgcgccaatt tcagtgtaat
     6001 caataccgcc gccgtccagt agtgtatcgg gtgcgccgat ggcgatattg gcctgattat
     6061 taataatcgc atcccagacg ccgttataca cttcggtcgt cacggtaatc tggcaggtag
     6121 gaaactgctt tttcagcacc tgtaataagc gcgcggtgtg tttgggcgta taaagcagct
     6181 gattaataca aatgcgcaca cgcgcttcta tgccctgcga aatagtatcg atactgcgtt
     6241 tgatggcgtg aaaatcattc agcaggtcgg tagctttgcg gaaaaaataa cgcccggact
     6301 ccgttaactc aatgctgcgg gtactgcgag tgaacagcac cacatccagc cccgtctcca
     6361 tacgcttaat ggtgtagcta atggcggaag tggttaaacc caactcttct gcggctttac
     6421 tgaaactgcc aaaacgcgca gcggtagtaa atgccaacag attctcttcg gtaaaaattg
     6481 agttcattaa accattcccg ccattatcta ctatgaattt ctccagctat gactgtagag
     6541 gtatcggtta aagatagata atcatttttg aataactttt aatacccgtc gcgtttagtc
     6601 gtcgcttcgc ttaatttttg tccaccatat tctgaaatta tccccacgca gttacccgcc
     6661 agaaaaccca gtaacgtcac cgtccagttc atcccgctgg caaaaaacag cgtcatgccg
     6721 agaaagccac cgggaataaa tgacagtaac cagaaacgcc cttgccagac caccaccgcc
     6781 gagaagggta cggtagtaat tacgctcgcc cagaacgcgg acagtccact tacagaggcc
     6841 agccaaccgg cagccagcgc ggcgaaaaac gcccacacca tcccggaata attaaccgcc
     6901 aggcttctga taaaccccga tttaccgccg cctgcggcat aaaaactgca aaaggcgacg
     6961 aagccaatgg ttcctaataa ttcccatccc tgacagagcc cgacatgtga agacactaac
     7021 tgccaaagcc ctgcacaaat accgacggtg acacccgttg ccgtgagtcc gttcatcttg
     7081 ttcccctctg gttagacatg gacgaaagct gcgcttatat gtgcagacta tagagaaata
     7141 aaacctgatt gagtgaaagg gtatcgggtc aaagaaataa atattgcact atcgcacact
     7201 gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa tacggcgaat taaatttaag
     7261 cgggttcttt tatcagatta tcgttgctat tctcaattca tcgaaaacag ccacaaaaac
     7321 caggggaatg tgaatgagta taaaaaatct acctgccgac tatttattgg ctgcacaaca
     7381 gggtgatatc gataaagtaa aaacctgcct tgcgctgggt gtcgatatta atacctgcga
     7441 tcgtcagggg aaaacggcaa ttacgctggc aagtttatat cagcaatatg cttgcgttca
     7501 ggtattaatt gacgccggag cggatattaa taaacaagat catacctgtt taaatccttt
     7561 tttaattagc tgtctgaacg atgatttaac gctactacga attattttac cggctaaacc
     7621 cgatcttaat tgcgtaaccc gttttggcgg tgtcggcctg acgcctgcct gtgaaaaagg
     7681 ccatttaagt attgtaaaag aacttttggc acatacggag attaacgtta accagaccaa
     7741 ccatgtaggc tggacgccgc tactggaagc gattgtgctt aatgatggtg gtattaaaca
     7801 gcaggcgatt gtccagttat tgctggaaca cggggccagc ccgcacatga cggataaata
     7861 tggcaaaacg ccgctggaac tggcgcggga acggggcttt gaagagattg cgcagttact
     7921 gattgccgca ggtgcataaa ccgggaggct tgctatcaac acaccagaaa aacggtgtgt
     7981 gtgggcgcta actgcggatg cggattttct ggcgcagcgg gggcaaggac aggttgaaca
     8041 ggtctttgcc agagcggtaa atatcgcact cccggctcgc cagcagttgc tgacgctgct
     8101 ttgtgaagag cacgacaatg cgccaaacag ttgtcggttg gcactcactc actttgatga
     8161 tctgttccgg catggtgata aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca
     8221 tcttcatata gagatgagtc gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc
     8281 tgtgaatttt cgccttatcg cctggcaaca gtggcacgac atcattcatc agcacctggg
     8341 ggaaaatgaa accctgttta attatcgcgg cgataatccg ttttatcagg cgttaaataa
     8401 agaattacat attaaacgac gggcagttat acaggcagta atcgataaac aaaatatcgc
     8461 ctcagcggtc gccagtatga tgggcttagg gattggcctt acgccatcag ccgatgatta
     8521 tttaacaggt ctggcactta ttttatttat ttccgggcat ccggcgggaa aatacaaaga
     8581 ggaattttat ctcggcttgc aacgcggcag aaataatacc acgttattaa gtgccataac
     8641 gctggaagcc gcattacaac aacgcttccg ggaaaatatt catcgtttta ttcacaacat
     8701 tatttatgac atccctggga acgcaactca ggcaatagaa aaaattaaac atattggctc
     8761 cagttccggc tgcgacatgc tgtatggcat ggccgatggt tgtgcgctga gccaaaccta
     8821 cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa tacctatttt gattctgtct
     8881 cgctgatgtc tatctccacg cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg
     8941 cgatggcgac cgaaatgaac aaaggtgtgc tgaaaaattt aggactgctg acgccggagc
     9001 tggaacaggc gaaaaacggc gacctgatga ttgtcatcaa tggtaaatcg ggggcggaca
     9061 acgagcagtt gctggtggag attgaagaac tgttcaacag caaagcgcaa agcggctcgc
     9121 acgaggcgcg ttacgccact attgccagcg ccaaaaagca tatcccggaa agtaacctgg
     9181 cggtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaatg
     9241 acctcaacgt gatgctgttt tccgataacg tctcagttga agatgaactg gcgctcaagc
     9301 aactggccca cgaaaaaggg ctgctgatga tggggccaga ctgtggcacg gcgattatca
     9361 acggcgcggc gctctgtttt ggtaacgccg tgcgtcgcgg caacatcggt attgttggcg
     9421 catccggcac cggcagccag gagctgagcg tccgcattca tgaatttggt ggcggcgttt
     9481 cacaactgat tggcaccggc gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc
     9541 tcgacgccat cgggatgctg gaaaacgatc cgcaaactga aatcattgcg ctgatctcca
     9601 aaccgcctgc gcctgcggtg gcccgcaaag tgctggaacg tgcgcgcgcc tgccgcaagc
     9661 cggtggtggt ctgcttcctc ggtcgtggcg aaacgtcagt ggatgagcag gggctacagt
     9721 ttgctcgcgg cagcaaagaa gcggcgctaa aagcggtgat gctctccggc gtgaaacagg
     9781 aaaatctcga cctacatacg cttaaccagc cgttgattgc ggatgtgcgt gcgcgtctgc
     9841 aaccgcagca gaaatacatt cgtggcctgt tctgcggcgg cacgttgtgc gacgaaacca
     9901 tgttcgcggt gatggaaaaa catggcgatg tctacagcaa cattcagccc gatccggaat
     9961 tccgcctgaa agatatcaac cgcagcatca aacacacctt cctcgacttt ggcgatgacg
    10021 acttcaccaa cggcaagccg catccaatga ttgaccccac caaccgcatc agtcgcttga
    10081 tcgaagaagc gcgcgatcca gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg
    10141 gatcgcacga agatccggtt ggttccacca tcgatgcgat caaagaagca aaagcgatcg
    10201 ccgcagccgg agggcgcgag ttgatcattc tcgcctatgt gctgggcacc gatctcgata
    10261 cgccatcgtt agaacaacaa agccagatgc tgcttgatgc cggggtaatt ctggcgagca
    10321 gcagcaccaa taccggattg ctggcgcgtg aatttatctg caaaggggag gaagcctgat
    10381 gagccagtca ctgtttagcc aaccattgaa cgttattaac gtcggcatcg ccatgtttag
    10441 cgatgacctg aaaaagcagc atgtagaagt gactcaactc gactggacgc cgccggggca
    10501 gggcaatatg caggtggtgc aggcgctgga caacattgcc gattcgccgc tggcagacaa
    10561 aatcgccgcc gctaaccagc aggcgctgga gcgcattatc cagtcgcatc cggtgctgat
    10621 tggttttgag caggcgatta acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc
    10681 cgggccgccg atcacctggg aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc
    10741 gctggtgttc gaaggactgg cgaaagatct cgacgaggcg gctgaactgg cggcttccgg
    10801 ggagatcact ttctcgccgt gtcacgagca cgactgcgtg ggatcgatgg cgggtgttac
    10861 ctcggcctcg atgtttatgc acatcgtaaa aaacaaaacc tacggcaaca tcgcttatac
    10921 caacatgagc gagcagatgg cgaagatttt gcgtatgggc gctaacgacc agagcgtgat
    10981 tgaccgcctg aactggatgc gtgatgtgca gggaccaata ctgcgcgacg cgatgaaaat
    11041 tatcggcgaa atcgatctgc gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg
    11101 ccataaccgc aataacgccg ggacgacgct gctgattcag gcgctgacgc cggggattat
    11161 ccaggcgggc tattccgtcg cgcaacagcg cgaagtgttt gagtttgttg ccagtagcga
    11221 ttacttctcc ggcccgacgt ggatggcgat gtgtaaagcg gcgatggatg cggcgcatgg
    11281 cattgaatac agcaccgtgg tcaccaccat ggcgcgtaat ggcgtcgagt tcggcctgcg
    11341 ggtcagcggc ctgccggggc agtggtttac cggcccggcg cagcaggtca tcggcccgat
    11401 gtttgccggt tataagccgg aagattcagg gctggatatt ggcgacagcg ccatcacgga
    11461 aacctacggt attggcggat ttgccatggc gaccgcgcct gcaatcgtcg cgctggtggg
    11521 tggcacggtg gaagaggcta tcgatttctc ccgtcagatg cgcgaaatca cccttggcga
    11581 aaaccccaac gtcaccattc cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat
    11641 cacccgcgtg ggtagcagcg gcattctgcc agtgatcaac accgccatcg cccataaaga
    11701 tgcgggcgtc ggcatgattg gtgcgggcat tgtgcatcca ccttttgcct gcttcgagaa
    11761 agccattctt ggctggtgcg aacgttacgg cgtctgacct acatgttcat gccggatgcg
    11821 gcgtgaacgc cttatccggc ctacgaatgg cgcgagaatc tgtaggcctg ataagcgtag
    11881 cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc ggcctacgag
    11941 tggcgcgaga atttgtaggc ctgataagcg tagcgcatca ggcatttgtc accattgccg
    12001 gatgcggcgt gaacgcctta tccggcctac gaatggcacg aaaatttgta ggcctgataa
    12061 gcgtagcgca tcaggcagtc tggcgttggt cataacccca tcaccctctg tagcggacat
    12121 aacaacatga aagagcttgt ggtcgttgcc attggtggca acagcattat caaagataac
    12181 gccagccagt cgattgagca tcaggcggag gcggtgaaag ccgtcgccga tacggtgctg
    12241 gaaatgctgg cttccgatta cgacattgtg ctgacccacg gtaacgggcc gcaggtcggg
    12301 ctggatttac gccgtgcgga gattgcccac gagcgcgaag ggctgccatt aacgccgctg
    12361 gcgaactgtg tggcagatac gcagggcggc atcggctatc tgatccaaca ggcgctgaat
    12421 aaccggctgg cgcgtcacgg cgagaaaaaa gccgtcaccg tggtgactga ggtggaagtg
    12481 gataaaaacg atccgggatt tgctcatccc accaagccca tcggcgcatt cttcagtgaa
    12541 agccagcgtg acgaattaca aaaggcaaac cctgactggt gttttgttga agatgccgga
    12601 cggggctatc gccgcgtggt cgcctcgccg gaaccgaaac gtattgtcga agcacccgcc
    12661 attaaggcgc tgatccaaca aggctttgtg gtgattggcg cgggcggcgg cggaattcct
    12721 gtggtgcgta ctgacgcggg agattaccaa agcgtggacg cggttatcga caaagatctc
    12781 tctaccgtgc tgctggcccg tgaaattcac gccgacattc tggtgatcac caccggcgtg
    12841 gaaaaagtgt gtattcattt tggcaaaccc gagcagcagg cgctcgatcg ggtggatatt
    12901 gccaccatga ctcgctatat gcaggaagga catttcccgc ccggcagcat gttgccaaaa
    12961 atcatcgcca gcctgacgtt cctggaacaa ggcggcaaag aagtgattat caccacgccg
    13021 gaaagcctgc ccgcggcgct gcgtggtgag acgggcactc atattattaa aacgtaagga
    13081 cgtaagatga aagaaagcaa tagccgccgt gagtttctga gccagagcgg taagatggtc
    13141 accgccgccg cgctgtttgg tacctctgtg ccgctcgccc atgcggcggt agctggcacc
    13201 ctaaactgcg acgcgaataa caccatgaaa atcactgacc cgcattacta tctcgacaac
    13261 gtgctgctgg aaactggatt tgactacgaa aatggcgtgg cggtgcagac ccgcacggcg
    13321 cgccagaccg tggagattca gaacggcaaa attgtcgcgc tgcgcgagaa taagcagcac
    13381 ccggatgcca cgctgccgca ctatgacgct ggcgataagc tgatgctgcc caccacccgc
    13441 gacatgcata ttcatctcga caaaaccttt tacggcgggc cgtggcgctc gctcaatcgc
    13501 ccggcaggca ccaccatcca ggacatgatc aaactcgagc agaaaatgct gccggagctg
    13561 caaccgtaca cgcaggaacg ggctgaaaaa ctgatcgatt tattacagtc gaaaggcacc
    13621 accattgccc gcagccactg caatatcgaa ccggtttccg gcctgaaaaa tctgcaaaat
    13681 ttgcaggcgg tgctggcgcg acgtcaggcg ggctttgagt gtgaaatcgt cgccttcccg
    13741 cagcacggtt tgctgctgtc gaaatcggaa cccttaatgc gtgaagcaat gcaggcgggt
    13801 gcgcattacg tcggcgggct ggacccgacc agtgtcgatg gcgcgatgga aaaatccctc
    13861 gataccatgt tccagattgc gctggactac gacaaaggcg tcgatattca cctgcacgaa
    13921 accactccgt cgggcgtggc agccatcaat tatatggttg aaacggtaga gaaaacgccg
    13981 caactgaagg gcaagctgac catcagccac gcctttgcgc tggctacgct caacgaacaa
    14041 caggtcgatg aactggcgaa tcggatggcg gcgcagcaaa tttctatcgc ctcgacggtg
    14101 ccgattggca cgctgcatat gccgctcaaa cagttgcacg acaaaggcgt aaaagtgatg
    14161 actggcactg acagcgttat cgaccactgg tcgccttatg gtctgggcga catgctggaa
    14221 aaagccaatc tctacgcgca gctctatatt cgtcctaacg aacagaacct ctcccgctcg
    14281 ctgtttttag ccactggtga tgtattgccg ctcaacgaaa aaggcgagcg tgtatggcca
    14341 aaagcgcagg atgacgccag ctttgtgctg gtggacgcct cctgttccgc cgaggcggtg
    14401 gcgcgtatct cgccgagaac cgcaacgttc cataaagggc aactggtgtg ggggagtgtg
    14461 gcaggttgat gcgcatttga aacgcctgca acggtgaata gtaagagatt taagccccag
    14521 ggagtggggc aatctgaata tgagtgtgtc ggcagaaatg gacattatct ttgaggaata
    14581 tgcccttatt gacgttgtgt agataactat ttgtctacgt gaaaagatca tcagttttgc
    14641 cgattttagt cttttacgga tagcaaatat cacacttata gacgctcgcc ttatggggaa
    14701 cgttgctgct ctgtttcatt caggctcgcg gcatgttggg gctggatcgg gtggtttaaa
    14761 tattggctat attcaatgga cgcgttttgc cgcgatgaca tatcaggcgt cgccaaatac
    14821 acatagctaa tcaggagtaa acacaatgaa gatcaaagct gttggtgcat attccgctaa
    14881 acaaccactt gaaccgatgg atatcacccg gcgtgaaccg ggaccgaatg atgtcaaaat
    14941 cgaaatcgct tactgtggcg tctgccattc cgatctccac caggtccgtt ccgagtgggc
    15001 ggggacggtt tatccctgcg tgccgggtca tgaaattgtg gggcgtgtgg tagccgttgg
    15061 tgatcaggta gaaaaacatg cgccgggcga tctggtcggt gtcggctgca ttgtcgacag
    15121 ttgtaaacat tgcgaagagt gtgaagacgg gctggaaaac tactgtgatc acatgaccgg
    15181 cacctataac tcgccgacgc cggacgaacc gggccatacg ctgggcggct actcacaaca
    15241 gatcgtcgtt catgagcgat atgttctgcg tattcgtcac ccgcaagagc aactggcagc
    15301 ggtagcccca ttactgtgcg cggggatcac tacttactcc ccgttgcgcc actggcaggc
    15361 cggaccgggt aaaaaagtgg gcgtggtcgg catcggcggt ctgggacata tggggatcaa
    15421 gctggcccac gcgatggggg cacatgtggt ggcatttacc acttctgagg caaaacgcga
    15481 agcggcaaaa gccctggggg ccgatgaagt tgttaactca cgcaatgccg atgagatggc
    15541 ggctcatctg aagagtttcg atttcatttt gaatacagta gctgcgccac ataatctcga
    15601 cgattttacc accttgctga agcgtgatgg taccatgacg ctggttggtg cgcctgcgac
    15661 accgcataaa tcgccggaag ttttcaacct gatcatgaaa cgccgtgcga tagccggttc
    15721 tatgattggc ggcattccag aaactcagga gatgctcgat ttttgcgccg aacatggcat
    15781 cgtggctgat atagagatga ttcgggccga tcaaattaat gaagcctatg agcgaatgct
    15841 gcgtggtgat gtgaaatatc gttttgttat cgataatcgc acactaacag actgaaaaaa
    15901 ttaataaata ccctgtggtt taacatatta acttcgctct ccacttaact ttttagttaa
    15961 ggagagcgaa ataatatcaa agtagcagta aaacctataa cgtaaattca aattgttaaa
    16021 ttaactccct ccagtacaca atacttcaca cgttagttat gagcgatttc tgatagtgcc
    16081 tggtttaatc agagctttat tatctgcgac gtttattttt atttaagaga gatcgcgatg
    16141 atatcattaa aagctccgca caataatttg atgccatata cgcaacaaag catacttaat
    16201 acggttaaaa acaatcagtt accagaggat atcaaaagct ccctggtttc ctgtgtcgat
    16261 atattcaagg ttttgattaa acaatattat gattatcctt atgattgtcg tgatgattta
    16321 gtcgatgacg acaaactcat tcatctcatg gctgccgtac gagactgtga gtggtcagac
    16381 gataacgcac tcaccataaa tgtgcagttt aatgatttcc ctggatttta tgactggatg
    16441 gattaccctg atcacccggt taagtttgtt tttcgcatac ttgaaaatca aaaagggaca
    16501 gtttgggtat atgaccagga tgatgcgttt cttgatatta aagcaaacgt gcaggctgga
    16561 cgctttaccg ggcttaagaa attagtgcag tttatcgatt cagtgcgaac agattgcaaa
    16621 tgtattcttc ttgaacacca tatgcctttg ctgagacttt tccctaaagg gaaagagtgt
    16681 atgcatgtgg aaaaatggct tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt
    16741 aagcaggtgc ttgcgcatgg attattatta cacttaaaaa atatctaccc tgtttttcct
    16801 gaatcactgg tgatgttatt gctctctgtt ctggacgtaa aaacatacag agatgacgca
    16861 cgattaaatg aatggatatc taatagagtg caggaattag gtgacagata ttatcctgta
    16921 aataagcacg ttaaaatccg ctacactctt tagttcagaa agggttccac ctgcaagttg
    16981 ttattccacc tgagagtgaa tcgcaatgca ggtaatcatt tcatctgtat atctgtatgc
    17041 caactggcat tccatctctt ttttcgccga tattttgttg tgatcggcga cacttcgctt
    17101 aaaaaagcac cagtagtggt ttcgcagcca tgcggtgtat aaaaaaaatg atctcatgca
    17161 gatgttttgt gaatgtgtgg gttgacattc atatgaaaaa aatcataatt ccatcatgtt
    17221 tgtgtggggt cttttctgta tcttacgcat cgcactcaag cctgacagaa aatatgctgt
    17281 aaggctcata tcaaaaaccg ccattagctc atcaggaaga gcagacgaca accaggattg
    17341 ttgtatggta cggggttcga ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat
    17401 cagatcccct tattatcatc cctgcaaagt ggaacattgc cattgtttgg ctattcagga
    17461 cgggtcagac caatgaaaaa agcccacaca ggggagagtg ggctgaaatg ggaagctaaa
    17521 gactcaagta aacttatcgg aaataaggac cacgcattac gggggctatc atcgccgatg
    17581 gcctggattc ggcaaatcag gttgattacg tagagagtaa attatctgct caccgctgcg
    17641 tcaccccttc gtaaatcagg cgtagcgcga atacaccaat aattgcacca ataacccgac
    17701 tggcaacgcg ttgcatacgc ccataagcac gacgcacagc gggcaaagaa aacgcctgac
    17761 taagaaagac tcgccagata actgatgcga gcacaatccc cgcccaggcc attaaacgtg
    17821 cccatgttgg tgtttcggca tttaatgtta ctgagaaaat actgataaaa aataaaacgg
    17881 tttgcgggtt agagagatcg gtaattaatc cgcggcgaaa aaagacatac cagggggcgc
    17941 taatcggttg ttgtagtgtg ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg
    18001 caaaccataa gagataagcg ccgccgacga ttctgataag cgaaaaaatc tcctcacact
    18061 gcgtaattag tgttgcaaga ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg
    18121 ccacgcccag cccggtcagc acccctgcgc gtcgaccgga agccaggctg gtttgtacta
    18181 ccacaaagag attggctccc ggattaaaaa aagtaatcac gaacagtccc acggtcaggt
    18241 aaacggcatg caaaggatcc atagtgattt catccataaa taagtgaact aactgcatca
    18301 tatttctacc aaaaataatc gggtgcgaga gagatcacaa agtgtcttat ttccggttac
    18361 tggcgtttat gccctgactg aactaattat taatcaaccc aataatgtgg gtgggtgata
    18421 gtgtgataac aactctggag ccgtaatatg aaaataatct ctaaaatgtt agtcggtgcg
    18481 ttagcgtttg ccgttaccaa tgtctatgcc gctgaattga tgaccaaagc ggaatttgaa
    18541 aaagttgaat cgcagtatga aaaaataggt gatatttcaa ccagtaatga aatgtcgact
    18601 gcagatgcaa aagaagattt aatcaaaaaa gccgatgaaa aaggggctga tgtgttggtg
    18661 ctgacctctg gtcaaactga caataagatc cacggcacgg cggatattta taagaagaag
    18721 taattctgaa ccctatgtaa acatccccga tgcgtaagtt tatcggtgat catcatctat
    18781 tgaaatttat gccggataaa gcgttcgcgc tgcattcggc agttcagctt ttcagccgcc
    18841 gccagaacgt cgtccgactg atgcctaaat aattcgccgc tgctgtttta tcgccattaa
    18901 atttctccag tgcctgttgt ggtgtcagta agcgtggagc gggagttttc gccgactcgc
    18961 gcgccagttc cggcagtagc agctgcaaaa attgcggcgt taaatccggc gtcggttcca
    19021 cacttaaaaa tagcgccagt cgctccatca tattgcgcag ttcacgaata ttgcccggcc
    19081 agtcgtagcg cagcagcaca gtttcacttg cctgtaaccc ctggcgtaat gcagcagaaa
    19141 acggggcgga gagcgccgcc agagacactt tcaaaaaact ttccgccagt ggcagaatat
    19201 ctgccacccg ctcgcgcagt ggtggcaatt gcagacgcaa aatactcaac cgataaaaca
    19261 gatcacggcg aaactgccct tgccgcatat cttcttccag attgcagtga gtggcgctaa
    19321 tgacccgcac atccaccgga acaggctgat gcccgccgac gcgagtaacc tctttttctt
    19381 ccagcacccg taacaggcga gtctgcaaag gtagcggcat ttcgccaatc tcatccaaaa
    19441 acagcgtgcc accgtgggcg atttcgaaca gtccggcgcg acctccgcgt cgcgagccgg
    19501 taaacgcccc ttcctcatag ccaaacagct ccgcttccag cagcgattcg gcaatcgccc
    19561 cgcagttgac ggcaacaaac ggatgcgact ttttgccctg tcgcgcatcg tggcgggcaa
    19621 aatattcccg atgaattgcc tgggccgcca gctctttgcc cgtccccgtt tccccctcaa
    19681 tcaacaccgc cgcactggag cgggcataca gcaaaatagt ctgccgtact tgttccatct
    19741 gtggtgattg accgagcata tcgcccagca cgtaacgagt acgcagggcg ttacgggtag
    19801 catcgtgagt gttatggcgt aacgacatgc gcgtcatatc cagcgcatcg ctgaacgcct
    19861 ggcgcacggt ggcggcggaa tagataaaaa ttccggtcat tccggcttct tctgccagat
    19921 cggtaatcag ccccgcgccg accaccgctt cggtgccgtt agcttttagc tcgttaatct
    19981 gcccgcgtgc gtcttcttcg gtaatgtagc tacgttgatc gaggcgcaaa ttaaaggttt
    20041 tttgaaacgc caccagtgcc ggaatagttt cctgataagt gacaacgccg atagaagagg
    20101 tgagctttcc ggcttttgcc agtgcttgta acacatcgta gccgctcggt ttaatcaaaa
    20161 taactggcac tgacaggcgg cttttcaggt acgcgccgtt agagccagca gcgatgatgg
    20221 cgtcacagcg ttcgtttgcc agtttcttgc ggatgtaggt cactgctttt tcaaagccaa
    20281 gctgaatagg ggtaatgttc gccaggtgat caaactcgag gctgatatcg cgaaacagct
    20341 cgaacaggcg cgttacagat accgtccaga taaccggttt gtcgtcattc agccgtggtg
    20401 gatgtgccat agcgcaccgc aaagttaaga aaccgaatat tgggtttagc cttgtttcat
    20461 aattgttgca atgaaacgcg gtgaaacatt gcctgaaacg ttaactgaaa cgcatatttg
    20521 cggattagtt catgacttta tctctaacaa attgaaatta aagatttaat tttattaagg
    20581 caattgtggc acaccccttg ctttgtcttt atcaacgcaa ataacaagtt gataacaaag
    20641 gatgggctat gtctctacac tctccaggta aagcgtttcg cgctgcactg tctaaagaaa
    20701 atccattgca gattgttggc accattaacg ccaaccatgc gctgttggcg cagcgtgccg
    20761 gatatcaggc aatttatctc tccggcggtg gcgtggcggc agggtcgctg gggctgcccg
    20821 atctcggtat ttctaccctt gatgatgtgc tgaccgacat tcgccgtatc accgacgttt
    20881 gttcgctgcc gctgctggtg gatgcggata tcggttttgg ttcttcggcc tttaacgtgg
    20941 cgcgtacggt gaaatcgatg attaaagccg gtgcggcagg attgcatatt gaagatcagg
    21001 ttggtgcgaa acgctgcggt catcgtccga ataaagcgat cgtctcgaaa gaggagatgg
    21061 tggatcggat ccgcgcggcg gtggatgcga aaaccgatcc tgattttgtg atcatggcgc
    21121 gcaccgatgc gctggcggta gaggggctgg atgcggcgat cgagcgtgcg caggcctatg
    21181 ttgaagcggg tgccgagatg ttgttcccgg aggcgattac cgaactcgcc atgtaccgcc
    21241 agtttgccga tgcggtgcag gtgccaatcc tcgccaacat taccgaattt ggcgccacgc
    21301 cgctgtttac caccgacgaa ttacgcagcg ctcatgtcgc gatggcgctg tacccgcttt
    21361 cagcgtttcg cgccatgaac cgcgccgccg aacatgtcta caacgtcctg cgccaggaag
    21421 gcacgcagaa aagcgtcatc gacaccatgc agacccgcaa cgagctgtac gaaagcatca
    21481 actactacca gtacgaagag aagctcgacg acctgtttgc ccgtaaccag gtgaaataat
    21541 aaacgcccgt tggttgtatt cgacaaccga tgcctgatgc gccgctgacg cgacttatca
    21601 ggcctacggg tgtactgaac tgtaggtcgg ataagacgca tggcgtcgca tccgacaaca
    21661 atctcgaccc tacaaatgat aacaatgacg aggacaacat gagcgacaca acgatcctgc
    21721 aaaacagtac ccatgtcatt aaaccgaaaa aatcagtggc gctttctggc gttccggcgg
    21781 gcaatacggc gctctgcacc gtgggtaaaa gtggcaatga cttgcattac cgtggttacg
    21841 atattcttga tctggcggaa cattgcgaat ttgaagaagt ggcgcatctg ctgatccacg
    21901 gcaaactgcc gacccgtgac gaactcgccg cctacaaaac aaaattgaaa gccctgcgcg
    21961 gtttaccggc taacgtgcgt actgtgctgg aagccttacc agcggcgtcg catccgatgg
    22021 atgtcatgcg caccggcgtt tctgcgatcg gctgcacgct gccagaaaaa gaggggcaca
    22081 ccgtttccgg tgcgcgggat attgccgaca aactgctggc atcacttagc tcgattctcc
    22141 tttattggta tcactacagc cataacggcg aacgcatcca gccggaaacc gatgacgact
    22201 ccatcggcgg tcacttcctg catctgctgc acggcgaaaa gccgtcgcaa agctgggaaa
    22261 aggcgatgca tatttcgctg gtgctgtacg ccgaacacga gtttaacgcc tccaccttta
    22321 ccagtcgggt gattgcgggc actggctctg atatgtattc cgcgattatt ggcgcgattg
    22381 gcgcactgcg cgggccgaaa cacggcgggg cgaatgaagt gtcgctggag atccagcaac
    22441 gctacgaaac gccagacgaa gccgaagccg atatccgcaa gcgggtggaa aacaaagaag
    22501 tggtcattgg ttttgggcat ccggtttata ccatcgccga cccgcgtcat caggtgatta
    22561 aacgtgtggc caagcagctc tcgcaggaag gcggctcgct gaagatgtac aacatcgccg
    22621 atcgcctgga aacggtgatg tgggagagca aaaagatgtt ccccaatctc gactggttct
    22681 ccgctgtttc ctacaacatg atgggcgttc ccaccgagat gttcacacca ctgtttgtta
    22741 tcgctcgcgt caccggctgg gcggcgcaca ttatcgaaca acgtcaggac aacaaaatta
    22801 tccgtccttc cgccaattat gttggaccgg aagaccgcca gtttgtcgcg ctggataagc
    22861 gccagtaaac ctctacgaat aacaataagg aaacgtaccc aatgtcagct caaatcaaca
    22921 acatccgccc ggaatttgat cgtgaaatcg ttgatatcgt cgattacgtc atgaactacg
    22981 aaatcagctc taaagtggcc tacgacaccg cacattactg cctgctcgac acgctcggct
    23041 gcggtctgga agctctcgaa tacccagcct gtaaaaaact gctggggcca attgtccccg
    23101 gcaccgtcgt acctaacggc gtgcgcgtcc ccggaactca gttccagctc gaccccgtcc
    23161 aggcggcatt taacatcggc gcgatgatcc gttggctcga tttcaacgat acctggctgg
    23221 cggcggagtg ggggcatcct tccgacaacc tcggcggcat tctggcaacg gcggactggc
    23281 tttcgcgcaa cgcggtcgcc agcggcaaag cgccgttgac catgaaacag gtgctgaccg
    23341 gaatgattaa agcccatgaa attcagggct gcatcgcgct ggaaaactcc tttaaccgcg
    23401 tcggcctcga ccacgttctg ttagtgaaag tggcttccac cgccgtggtc gccgaaatgc
    23461 ttggcctgac ccgcgaggaa attctcaacg ctgtttcgct ggcgtgggtg gacggtcagt
    23521 cgctgcgcac ctatcgccat gcgccgaaca ccggcacgcg taaatcctgg gcggcgggcg
    23581 atgccacttc ccgcgcggta cgtctggcac tgatggcgaa aacgggcgaa atgggttacc
    23641 cgtcagccct gactgcgccg gtatggggct tctacgacgt ctcctttaaa ggtgaatcgt
    23701 tccgcttcca gcgtccgtac ggttcttacg tcatggagaa tgtgctgttc aaaatctcct
    23761 tcccggcgga gttccactcc cagacggcag ttgaagcggc gatgacgctc tatgaacaga
    23821 tgcaggcagc aggcaaaacg gcggcggata tcgaaaaagt gaccattcgc acccacgaag
    23881 cctgtattcg catcatcgac aaaaaagggc cgctcaataa cccggctgac cgcgaccact
    23941 gcattcaata catggtggcg atcccgctgt tgttcgggcg cttaacggcg gcagattacg
    24001 aggacaacgt tgcgcaagat aaacgcatcg acgccctgcg cgagaagatc aattgctttg
    24061 aagatccgac gtttaccgct gactaccacg acccggaaaa acgcgccatc gccaatgccg
    24121 ttactcttga gttcactgac ggtacgcgct ttgaagaagt ggtggtggag taccccattg
    24181 gtcatgctcg ccgccgtcag gatggcattc cgaagctggt agataaattc aaaatcaatc
    24241 tcgcgcgcca gttcccgact cgccagcagc agcgcattct ggaggtttct ctcgacagag
    24301 ctcgcctgga acagatgccg gtcaatgagt atctcgacct gtacgtcatt taagtaaacg
    24361 gcggtaaggc gtaagttcaa caggagagca ttatgtcttt tagcgaattt tatcagcgtt
    24421 cgattaacga accggagcag ttctgggccg agcaggcccg gcgtattgac tggcagacgc
    24481 cctttacgca aacgctcgat cacagcaatc cgccgtttgc ccgttggttt tgtgaaggcc
    24541 gaaccaactt gtgtcacaac gctatcgacc gctggctgga gaaacagcca gaggcgctgg
    24601 cattgattgc cgtctcttcg gaaacagaag aagagcgcac ctttaccttt cgccagctgc
    24661 atgacgaagt gaacgcggtg gcgtcaatgc tgcgctcact gggcgtgcag cgtggcgatc
    24721 gggtgctggt gtatatgccg atgattgccg aagcgcatat taccctgctg gcctgcgcgc
    24781 gcattggcgc tattcactcg gtggtgtttg ggggattcgc ctcgcacagt gtggcggcgc
    24841 gaattgatga cgctaaaccg gtgctgattg tctcggcaga tgccggagcg cgcggtggca
    24901 aaatcattcc ctataaaaaa ttgctcgacg atgcgataag tcaggcgcag catcagccgc
    24961 gccatgtttt gctggtagat cgtgggctgg cgaaaatggc gcgcgttagc gggcgggatg
    25021 tcgatttcgc gtcgttgcgc catcagcaca tcggcgcgcg ggtgccggtg gcctggctgg
    25081 aatcaaacga aacctcctgc attctctaca cctccggcac gaccggcaaa cctaaaggcg
    25141 tgcagcgtga cgtcggcgga tatgcggtgg cgctggcgac ctcgatggac accatttttg
    25201 gcggcaaagc gggaggtgtg ttcttttgtg cttcggatat cggctgggtg gtggggcatt
    25261 cgtatatcgt ctacgcgccg ctgctggcgg ggatggcgac tatcgtttac gaaggattac
    25321 cgacctggcc ggactgcggc gtgtggtgga aaatcgtcga gaaatatcag gttagccgga
    25381 tgttctcagc gccgaccgcc attcgcgtgc tgaaaaaatt ccctaccgct gaaattcgca
    25441 aacacgatct ctcgtcgctg gaagtgctct atctggctgg agaaccgctg gacgagccga
    25501 ccgccagttg ggtgagcaat acgctggatg tgccggtcat cgacaactac tggcagaccg
    25561 aatccggctg gccgattatg gcgattgctc gcggtctgga cgacaggccg acgcgtctgg
    25621 gaagccccgg cgtgccgatg tatggctata acgtgcagtt gcttaatgaa gtcaccggcg
    25681 aaccgtgtgg cgtcaacgag aaagggatgc tggtggtgga agggccgctg ccgccggggt
    25741 gtattcagac catctggggc gacgacgacc gctttgtgaa gacgtactgg tcgctgtttt
    25801 cccgcccggt gtacgccacc tttgactggg gcatccgcga tgctgacggt tatcacttta
    25861 ttctcgggcg cactgacgat gtaattaacg ttgccgggca tcggctgggg acgcgcgaga
    25921 ttgaagagag tatctccagt catccgggcg ttgccgaagt ggcggtggtt ggggtgaaag
    25981 atgcgctgaa agggcaggtg gcggtggcgt ttgtcattcc gaaagagagc gacagtctgg
    26041 aagatcgtga tgtggcgcat tcgcaagaga aggcgattat ggcgctggtg gacagccaga
    26101 ttggcaactt tggccgcccg gcgcacgtct ggtttgtctc gcaattgcca aaaacgcgat
    26161 ccggaaaaat gctgcgccgc acgatccagg cgatttgcga aggacgcgat ccgggggatc
    26221 tgacgaccat tgatgatccg gcgtcgttgg atcagatccg ccaggcgatg gaagagtaag
    26281 gtattgtcgg atgcgtcgcg tggcgcatcc gacagttgtg cgcagatgcc tgatgcgacg
    26341 ctgg
//
LOCUS       DAZGCQ010000068        26057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000068 DAZGCQ010000000
VERSION     DAZGCQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.068"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHK07_003109"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHK07_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO8103405.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHK07_003110"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHK07_003110"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO8103406.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="ACHK07_003111"
     CDS             complement(938..1405)
                     /locus_tag="ACHK07_003111"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO8103407.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHK07_003112"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHK07_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO8103408.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHK07_003113"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHK07_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO8103409.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHK07_003114"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHK07_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO8103410.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHK07_003115"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHK07_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO8103411.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHK07_003116"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHK07_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO8103412.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHK07_003117"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHK07_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO8103413.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHK07_003118"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHK07_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO8103414.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHK07_003119"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHK07_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO8103415.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHK07_003120"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHK07_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO8103416.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHK07_003121"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHK07_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO8103417.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHK07_003122"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHK07_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO8103418.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHK07_003123"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHK07_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO8103419.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHK07_003124"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHK07_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO8103420.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHK07_003125"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHK07_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO8103421.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHK07_003126"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHK07_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO8103422.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHK07_003127"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHK07_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO8103423.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHK07_003128"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHK07_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO8103424.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHK07_003129"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHK07_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO8103425.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHK07_003130"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHK07_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO8103426.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHK07_003131"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHK07_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO8103427.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHK07_003132"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHK07_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO8103428.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHK07_003133"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHK07_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO8103429.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHK07_003134"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHK07_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO8103430.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHK07_003135"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHK07_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO8103431.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHK07_003136"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHK07_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO8103432.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHK07_003137"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHK07_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO8103433.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHK07_003138"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHK07_003138"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO8103434.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHK07_003139"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHK07_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO8103435.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHK07_003140"
     CDS             15545..15913
                     /locus_tag="ACHK07_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO8103436.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHK07_003141"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHK07_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO8103437.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHK07_003142"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHK07_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO8103438.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHK07_003143"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHK07_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO8103439.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHK07_003144"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHK07_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO8103440.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHK07_003145"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHK07_003145"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO8103441.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDNWLALLDEAGMKGFPHTDYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHK07_003146"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHK07_003146"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO8103442.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHK07_003147"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHK07_003147"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO8103443.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHK07_003148"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHK07_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO8103444.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHK07_003149"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHK07_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO8103445.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHK07_003150"
     CDS             23092..23634
                     /locus_tag="ACHK07_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO8103446.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPICGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHK07_003151"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHK07_003151"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO8103447.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVAALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHK07_003152"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHK07_003152"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO8103448.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHK07_003153"
     CDS             25031..25288
                     /locus_tag="ACHK07_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO8103449.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHK07_003154"
     CDS             complement(25264..25818)
                     /locus_tag="ACHK07_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO8103450.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6684 a         6166 c         6763 g         6444 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaaccgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccacaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaatgattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta caacgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaatgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcag cggagggaag caattcagct tctggatatc ctgagaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tggacaaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctagagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact gacaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggcgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccttgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgtaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tctagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggtccgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag agttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg tatgtgggaa gcctttcatt
    19261 cctgcttcat ccagcaatgc aagccagttg tcgcgggaat gatgcgtacg attaatgcgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgagcatc actggcatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttgg aagagtggac tatcaggcca gacggttgcc cggaacaaac
    19921 cattcccgac gagaattcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aatgagagca ggttcaggat tccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagca tcagcagctt
    21541 gtacggcgat gatatggtcc gtatcgctca ctgggtggca aaacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 gcgtcgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc cattagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cactgaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg
    22741 ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc
    22801 gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt
    22861 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt
    22981 tgttcaacat cccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcaaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
    23281 tgccccatat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cttgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgcgca
    23881 gcgtgaaacc attttttccc gctggccagg tccagtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gctgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24481 tgcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 agacgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgcg cgatctctgg
    25141 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtat gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
    26041 gtcggaacaa cgaagag
//
LOCUS       DAZGCQ010000069        25904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000069 DAZGCQ010000000
VERSION     DAZGCQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.069"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..224)
                     /gene="shoB"
                     /locus_tag="ACHK07_003155"
     CDS             complement(144..224)
                     /gene="shoB"
                     /locus_tag="ACHK07_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO8103451.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(645..1025)
                     /gene="acpS"
                     /locus_tag="ACHK07_003156"
     CDS             complement(645..1025)
                     /gene="acpS"
                     /locus_tag="ACHK07_003156"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO8103452.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(1025..1756)
                     /gene="pdxJ"
                     /locus_tag="ACHK07_003157"
     CDS             complement(1025..1756)
                     /gene="pdxJ"
                     /locus_tag="ACHK07_003157"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO8103453.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRNACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1768..2496)
                     /gene="recO"
                     /locus_tag="ACHK07_003158"
     CDS             complement(1768..2496)
                     /gene="recO"
                     /locus_tag="ACHK07_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO8103454.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2508..3413)
                     /gene="era"
                     /locus_tag="ACHK07_003159"
     CDS             complement(2508..3413)
                     /gene="era"
                     /locus_tag="ACHK07_003159"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO8103455.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3410..4090)
                     /gene="rnc"
                     /locus_tag="ACHK07_003160"
     CDS             complement(3410..4090)
                     /gene="rnc"
                     /locus_tag="ACHK07_003160"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO8103456.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4363..5337)
                     /gene="lepB"
                     /locus_tag="ACHK07_003161"
     CDS             complement(4363..5337)
                     /gene="lepB"
                     /locus_tag="ACHK07_003161"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO8103457.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5353..7152)
                     /gene="lepA"
                     /locus_tag="ACHK07_003162"
     CDS             complement(5353..7152)
                     /gene="lepA"
                     /locus_tag="ACHK07_003162"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO8103458.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7350..7829)
                     /gene="rseC"
                     /locus_tag="ACHK07_003163"
     CDS             complement(7350..7829)
                     /gene="rseC"
                     /locus_tag="ACHK07_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO8103459.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7826..8782)
                     /gene="rseB"
                     /locus_tag="ACHK07_003164"
     CDS             complement(7826..8782)
                     /gene="rseB"
                     /locus_tag="ACHK07_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO8103460.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8782..9432)
                     /gene="rseA"
                     /locus_tag="ACHK07_003165"
     CDS             complement(8782..9432)
                     /gene="rseA"
                     /locus_tag="ACHK07_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO8103461.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9465..10040)
                     /gene="rpoE"
                     /locus_tag="ACHK07_003166"
     CDS             complement(9465..10040)
                     /gene="rpoE"
                     /locus_tag="ACHK07_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO8103462.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10037..10192)
                     /gene="rseD"
                     /locus_tag="ACHK07_003167"
     CDS             complement(10037..10192)
                     /gene="rseD"
                     /locus_tag="ACHK07_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO8103463.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10448..12070
                     /gene="nadB"
                     /locus_tag="ACHK07_003168"
     CDS             10448..12070
                     /gene="nadB"
                     /locus_tag="ACHK07_003168"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO8103464.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVHGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12055..12792)
                     /gene="trmN"
                     /locus_tag="ACHK07_003169"
     CDS             complement(12055..12792)
                     /gene="trmN"
                     /locus_tag="ACHK07_003169"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO8103465.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12924..14258
                     /gene="srmB"
                     /locus_tag="ACHK07_003170"
     CDS             12924..14258
                     /gene="srmB"
                     /locus_tag="ACHK07_003170"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO8103466.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14291..15172)
                     /gene="yfiE"
                     /locus_tag="ACHK07_003171"
     CDS             complement(14291..15172)
                     /gene="yfiE"
                     /locus_tag="ACHK07_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8103467.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15275..15862
                     /gene="eamB"
                     /locus_tag="ACHK07_003172"
     CDS             15275..15862
                     /gene="eamB"
                     /locus_tag="ACHK07_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO8103468.1"
                     /translation="MTPTLLSAFWTYTLITALTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISYSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15917..16300)
                     /gene="grcA"
                     /locus_tag="ACHK07_003173"
     CDS             complement(15917..16300)
                     /gene="grcA"
                     /locus_tag="ACHK07_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO8103469.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16605..17294
                     /gene="ung"
                     /locus_tag="ACHK07_003174"
     CDS             16605..17294
                     /gene="ung"
                     /locus_tag="ACHK07_003174"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO8103470.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17342..18379)
                     /gene="yfiF"
                     /locus_tag="ACHK07_003175"
     CDS             complement(17342..18379)
                     /gene="yfiF"
                     /locus_tag="ACHK07_003175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8103471.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18586..19005
                     /gene="trxC"
                     /locus_tag="ACHK07_003176"
     CDS             18586..19005
                     /gene="trxC"
                     /locus_tag="ACHK07_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO8103472.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19074..19772
                     /gene="tapT"
                     /locus_tag="ACHK07_003177"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19074..19772
                     /gene="tapT"
                     /locus_tag="ACHK07_003177"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO8103473.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19804..22464
                     /gene="pat"
                     /locus_tag="ACHK07_003178"
     CDS             19804..22464
                     /gene="pat"
                     /locus_tag="ACHK07_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO8103474.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22578..23933
                     /gene="pssA"
                     /locus_tag="ACHK07_003179"
     CDS             22578..23933
                     /gene="pssA"
                     /locus_tag="ACHK07_003179"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO8103475.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23979..24302
                     /gene="yfiM"
                     /locus_tag="ACHK07_003180"
     CDS             23979..24302
                     /gene="yfiM"
                     /locus_tag="ACHK07_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO8103476.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24299..25597)
                     /gene="kgtP"
                     /locus_tag="ACHK07_003181"
     CDS             complement(24299..25597)
                     /gene="kgtP"
                     /locus_tag="ACHK07_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO8103477.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25714..>25904)
                     /locus_tag="ACHK07_003182"
     CDS             complement(25714..>25904)
                     /locus_tag="ACHK07_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103478.1"
                     /translation="AILTDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHLHTC
                     SPHQKFILKLLNNHFPAN"
BASE COUNT         6266 a         6838 c         6452 g         6348 t
ORIGIN      
        1 ctattatatt tatttaattg atgattatta gcacaaacat tcactaagtt aatttatata
       61 gtatctgccc agacacttat ttatagttat taaaggtgcg tccgactggt tcaccggacg
      121 caccttaagt acgtttcctt gtgttataag aacagaagga tcagctgtaa aacagcaatg
      181 atgattttga tggcccgttt aataaggtat cggcaatcag tcattcgttt ttccttaaac
      241 aaggaatgca gccgttcaga ttagccatta catctcccca aaactgaacg tgcgagttat
      301 tgagggtgca tgctgcactc cacaccagag ctttgacgac accactcgtt tcaatggggg
      361 aattctgtgg catggtgtaa agcacagcaa aatcgccaat aacgaaacca attttaatgt
      421 acttacgaat tggcggtcac gttaatcttg ccatcaatat cgctttcttc gtaaaggctc
      481 gagtttttat gataaagatt gcaagttgct tgtaaaagat aactaccctg atccataatc
      541 gctgttgttg agggtgcgtg ctgcactaaa ttaaagttac aaagtaaaac ccccgttcct
      601 taccagttcg ggggttttac tttttaaaga gaacggtatt atttttaact ttcaataatt
      661 accgtggcgc aagcatagtg gcgctcatcg gccagcgtca catgcatatt tgcaacgccc
      721 agcttttccg ccagttttaa tgcctcgccc cacagtcgca gccgtggctt accaagctca
      781 tcgttaaata cttcaaactg attaaacgcc agaccattgc ggatcccggt gccaaacgct
      841 tttgctgcgg cttcttttac ggcaaagcgt ttcgccagaa aacgcaccgg ctggtggtgc
      901 gttttccaaa tttcccattc gttatcgctt aatacgcggc gtgccaggcg ctcaccggat
      961 cgggcgatca ctgcttctat gcgagcgatc tcgacgatat ccgtgcctaa acctaatatt
     1021 gccattagcc acgcgcttcc agcatcaggc gcttcatttc tgccaccgca tctttcagtc
     1081 cggtcatcac tgcacgacca ataatggcat gaccgatatt cagttcgtgc atctcaggga
     1141 tggcggcaat ggcttttacg ttgtgatagg tcagaccgtg tccggcgtta actttcaaac
     1201 cgagactagc ggcaaaggtc gcggctttgg cgatacgcgc caactcttgc gcctgttcgg
     1261 cgtcagtttt ggcatcagca tagcaaccgg tgtggatctc gataaacggt gcgccaacct
     1321 ctgccgcagc tttgatctgc tcttcatcgg cgtcgataaa cagagaaacc tgaatcccgg
     1381 catccgccag acgtttgcag gcattgcgca ttttttcacg ctgccctgca acgtccaggc
     1441 cgccttcggt tgttacttcc tgacgctttt ctggtaccag gcagcaaaaa tgcggcttcg
     1501 tctcaaccgc gatcgccagc atctcttcgg tcaccgccat ctccagattc atgcgggtat
     1561 ccagcgtctg acgcaggatg cgcacatcgc ggtcagtaat gtggcggcga tcttcacgta
     1621 aatgcacggt aatgccgtcc gcccccgcct gctcggcaat aaacgcggcc tgcaccggat
     1681 ccgggtaagc ggtaccgcgc gcgttgcgca gcgtagcgat atggtcaatg ttgacgccta
     1741 acagtaattc agccatgaca atcctcatca ttcataatgt gttttcaccg ttcgcttagg
     1801 cataaattgc cggaacagtt ccctgctctt taaaggttta ccgccaagat acggcttaag
     1861 cgccatgcgg gtaaagcgtt tcgcggcgcg cagtgtgtct gcgtcaggaa attcccgtgc
     1921 gtttaacgct tttaactgcc ttccggtgaa cgttttattg tcgataacga cgcttgcgat
     1981 aaaccctttt tcttcgcgat aacgatacgt catggtgtca tctaccggct cgccgctacc
     2041 cgcacaatgg gtaaaattga cgccataacc cagatgcccg agcagtgcca gttcaaagcg
     2101 gcgcagcgcg ggttctggcg taccagtgac ccctgcaaga gactgaatgc agtgcaagta
     2161 atcgaaaaag agttcagaga agcgcgtctc gtattccagt acgcgggaga gaagttcgtt
     2221 gatgtacaga ccgctgtaaa gcgtgatacc gcttaatggc agcgccagcg agacagcttc
     2281 agcactgcgc agcgttttga cttcgccacg cccgccaaaa cgtagcaaga gaggggtgaa
     2341 aggctgtaat gcacctttca gggttgagcg tttagagcgt gcgcctttgg caaccagacg
     2401 cacgcgaccc gattcctccg tgaagacgtc cagcatcagg ctggtttcgc tccacgggcg
     2461 actatgcagg acaaatgcgc gctgccagcc ttccatcgga gttactctta aagatcgtca
     2521 acgtaaccga gactgcgcag tgcgcgttcg tcgtcggccc aaccggattt cacttttacc
     2581 cacagctcaa ggtgaacagg cgcttcaaac atttcctgca tgtctttacg cgcttcaatc
     2641 ccgatggttt tgatcttggc ccctttgttg ccaatgacca tcttcttctg cccttcacgc
     2701 tcaacgagaa tcaaaccgtt gatgtcataa ccaccgcgtt cgttagagac gaaacgttcg
     2761 atctccacgg tcacggagta cggcagttca gcgccgagga aacgcatcag tttttcgcgg
     2821 atgatttcag acgccataaa acgctgtgag cgatcggtga tgtaatcttc cgggaagtga
     2881 tgagtcgctt caggtagatg cttacgcacg attgccgcaa tagtgtcaac attcagcccg
     2941 gtttcggcag agattggcac gatatcgagg aagttcatct ggcttgccag aaactgcagg
     3001 tgcggcagca gatcggcttt ctcctgcacg ttgtccactt tgttcaccgc gaggattacc
     3061 ggcgctttgc cttcgcgcag tttgttgagc accatttcgt cgtccggcgt ccagcgggtg
     3121 ccttcaacga caaaaatcac cagctcaaca tcgccaatag agctgctcgc cgctttgttc
     3181 atcaggcggt taatggcgcg tttttcttcc atatgcaggc ccggtgtatc gacgtagatc
     3241 gcctgatacg cgccttcagt atggatcccc acaatgcggt gacgagttgt ctgcgccttg
     3301 cgggaggtga tggagatttt ctgccccagc agtttgttca acaatgtgga tttgccaacg
     3361 ttcggacgtc cgacgatggc aataaatccg cagtaacttt tatcgatgct cattccagct
     3421 ccagtttttt caacgcctgt tcggcggcag cctgctcagc cttacgacgg cttgaacctg
     3481 tgccaaccac cggttcactc aggccgctga cctggcagtg gattgtaaat tcctgatcgt
     3541 gcgcttcgcc acgtacctgg actaccagat aagtcggcag cggcagatga cgaccctgca
     3601 aatattcttg caagcgcgtt ttcggatctt tttgtttatc gcctgggcta atttcgtcca
     3661 gacgagtttg ataccagttg aggattaatt tctcgacggt ttgaatatca ctgtcgagga
     3721 atacgccacc aattaatgct tcgacggtgt cggcgagaat tgactcacga cgaaatccac
     3781 cgcttttaag ttcacctggt cctaaacgta agcactcgcc taactcaaat tcgcgcgcca
     3841 gttccgccag cgtattgcca cggaccagcg tggcgcgcat ccggctcata tcgccttcat
     3901 ccacacgagg gaaacggtga taaagcgcat tggcgataac gtagctcaga atagagtcgc
     3961 ctaaaaattc taaacgctcg ttatgtttgc tgctggcact acgatgagtt aatgcctgct
     4021 gcaacagttc ctgatgatta aaagtgtagc ccagcttccg ttgaagccga ttaattacga
     4081 tggggttcat gcgataccaa taaataaatg cgtcaacaat tcagcacacg aaacagacct
     4141 gatatacatg gctctgctaa ctgcttcgct gcagtttcag tttagcggta tatggggacc
     4201 aacgctgttt cgtgtgccgt ggcaacctgg aggtgccaac cttaaacttc gggggaatat
     4261 tctatacaca acgacggggg atgtcgttag ccacgggaga tttatctcat aaataattca
     4321 cgttgtcgcc ataacggcga caacgtgaac gaagatgagc tattaatgga tgccgccaat
     4381 gcgacttaag cgcacacctg tcggccattc gccttcttgc ttatcgaagc tcatccagat
     4441 agccgttgcc cgaccgacca gattcgcttc aggcacaaag ccccagtaac ggctgtccgc
     4501 gctgttgtcg cggttgtcgc ccatcatgaa gtattgtccc ggaggaacaa tccaggttgc
     4561 cagttgttgc cctggctgct ggtaatacat ccccacctga tcctgcgcaa tcggcactgt
     4621 cagaatgcgg tgcgtcacat cacccagtgt ctctttacgc tcggaaagac gaattccatt
     4681 ttctttggtt tcgtttttcg gcacttcaaa gaatccgctg gtcgcttccc caccattacg
     4741 gcgtgagaag gtctgaacga aatcgctcgg ttccacgttt gagtaggtga ccggcagcgc
     4801 gttttcacac gcctggccgg aactgcatcc cggttgaatc gtcagctctt ttgagaccgg
     4861 atcgtaagtg actttatcgc ccggtaaacc caccgcgcgc ttgatgtaat caagctttgg
     4921 atcttccgga tatttaaaga ccacgatatc gccgcgtttc ggatgaccgg tttcgatcag
     4981 cgttttctgg tagataggat ctttaatgcc ataagcaaac ttctctacca gaataaaatc
     5041 accgattaac agagtcggca tcatcgaacc tgacggaatc tggaacggtt cataaataaa
     5101 cgaacgcaca atcaatacga tagccagtac cggaaaaaca gaagcaccgg tttccagcca
     5161 gccaggcttc ggcgcaacct ttttcaacgt tgctttatcc agtgagtcac cggcagccgc
     5221 ctgcgccgct gcctgacgtt cccgccgttt aggtgcgaaa aagaatttat ccacgcacca
     5281 taaaatgccc gtcaccagtg tggcaatcac cagaatcagg gcaaacatat tcgccatgcc
     5341 aactcctaag ggttatttgt tgtctttgcc gacgtgcaga atggcgagga acgcttcctg
     5401 cggcagctcg acgttaccga tctgcttcat gcgtttctta ccttctttct gcttctgcag
     5461 cagctttttc ttacggctga tatcgccgcc ataacattta gccagtacgt ttttacgcag
     5521 ctgtttcacg gtggatcgcg caataatgtg cgtaccaatc gctgcctgaa tggcgatatc
     5581 aaactgctgg cgtgggatca gatctttcat cttctccacc aactcgcgac cgcggttttg
     5641 cgaattatca cggtgggtga tcaacgccag cgcatcaaca cgttcaccgt tgattaatac
     5701 gtctacacgt accatgtcgg acgcctggaa gcgtttgaag ttgtaatcca aagacgcata
     5761 gccacgcgag gttgatttca gtcggtcgaa gaaatcaagc accacttccg ccatcgggat
     5821 ctcgtacgtc agcgccacct gattaccgtg gtaaaccata ttggtctgca cgccgcgttt
     5881 ttctacgcac agcgtaataa cgttgccgag atatgcctgc ggcagcagca tgtgacactc
     5941 tgcaatcggc tcgcgcagtt cgtaaatgtt atttaccgca ggcagcttgg atgggctgtc
     6001 gacgtagata acttcccttg acgtggtttc aacttcatac actacggtcg gcgcagtggt
     6061 gatcagatcc agatcgtatt cacgttccag acgctcctgg atgatctcca tgtgcagcag
     6121 gccaaggaag ccgcagcgga aaccaaagcc cagcgcgctg gagctttccg gctcatagaa
     6181 cagtgaggca tcgttcaggc tgagtttacc cagcgcgtca cggaaggctt catagtcgtc
     6241 ggaacttacc gggaacagac cggcgtatac ctgcggtttg actttcttaa agccaggcag
     6301 cgccttttct gccggattac gcgccagcgt taaggtatcg ccgactggag cgccgtggat
     6361 atctttaatc gcacatacga gccagcctac ttcgccacat ttcagttcag tgcggtcaac
     6421 ctgtttcggc gtgaagatgc ccagacggtc ggcgttatag gtctgcccgg tactcatgac
     6481 tttcactttg tcgcccttac gcagggtgcc gtttttaata cggataagtg aaacaacgcc
     6541 caggtagttg tcgaaccatg agtcgataat tagtgcctgc aacgggcctt ccggatcgcc
     6601 ttccggcggc ggaatgtcgc gcaccagacg ttcgagaacg tcctgcacgc caacgccggt
     6661 tttcgctgaa cagcgcaccg cgtcagtggc gtcgatgccg acgatatctt caatttcttc
     6721 cgccacgcgt tcaggatcgg ctgccggcag gtcaatcttg ttcagtaccg gcactacttc
     6781 gagatccatc tccatggcgg tgtagcagtt cgccagagtt tgcgcttcca cgccctgccc
     6841 ggcgtcgacc accagcaatg cgccttcgca ggccgccagc gaacgggaaa cttcatagga
     6901 gaagtcaacg tggcctggag tgtcgataaa gttaagctga taggtttcgc cgtcagacgc
     6961 tttgtagtcc agcgtcacgc tttgcgcttt gatggtaatg ccacgctcac gctcaagatc
     7021 catggaatcg agaacctgcg cctccatttc acggtcagac aggccaccgc agatctggat
     7081 aatacggtca gacagcgtcg atttaccgtg gtcaatgtga gctatgatcg aaaagttacg
     7141 tatattcttc ataaagtatg attattgtgc cttaatgccc ggttaaccag gcttttagaa
     7201 gtcgctgttc tgagcttaac gtctgtatta atagaaacgc cgcattctac actacaacat
     7261 tgaggcgagg aaatgttcat accgtatgga ttgtggtatc tggaaacgtc ctcgcatttg
     7321 ttatgcaaaa tgcaacaaag ccagtgaaat cactggctcg cgtcttccga agatgtttca
     7381 aatcgcacaa ggccaggcgg caatgccacg cttaagatga tcggttgcca ttctgcccgg
     7441 gcagcaaact tacgcgagta gccacgcgca atcaggaacc cgccaatacc gccgaggacc
     7501 gcaccacata atgctgcgac gtcagaagca aagagtagct gaaatagcga agcgatgagg
     7561 aataatccca ccagcggcga catataaacc agtaatgcgg agctaagcag gctgccttcg
     7621 gcgatcccca attccacttt ttgccccggc actaacggtt catcacaggg tacgacaatg
     7681 gtatgcgtgg tttgcgggcc aagtttattc aacacgcggc taccgcaacc ggcgcgtgaa
     7741 gcgcagctgc tgcatgaggc tttaacatca caactgacca gcgcctgccc gttttgccag
     7801 gagacgacgg tagcccactc tttgatcatt gcgctgcccc gaacttaata ttctcggcaa
     7861 tgcgtttcgc cgtttgcggc ggcagttcac cgacaatggt gatttcggcg ttatcacgta
     7921 cgcttgtact gacggttctg cgtccggtgc gcaacatctg atcggtgctc gatggcgtag
     7981 cgcggttaac gtttaccgag aagctgaata atccgtcgga atagagacgt gattcgatag
     8041 gcatgttgtc catcgtcggt agcggacgtc gactactgga aacttcgcta aaaccctgtg
     8101 gcaaccaggt tggcgtccag ctgaatttag ctttttcacc tacaggaaca gaaagcaacg
     8161 gcggcaaatt tgccttcgcc agcgtctgca tactgctgct gatatcctga ttgacgttaa
     8221 aagcaatcac gcgaaattgt tccagcgttt caccatcgcg atcaagaaga tcaacccgca
     8281 tcggtaattt cgattcggtg tccatccaca cgatgtagct gtagcgtgtg ccatcacgag
     8341 caaccacgcg aataacttca caaagacgat cagcaatacg cgtgcgcccg acggagataa
     8401 agtcgtagta aggagaaagg cgtttgaaat cggtatagat gagcgatggc agagaatcaa
     8461 caatgtaatc gccattaaga gtaaacggtt caaggcccgg ttcaaaatag ctgatttcat
     8521 tgccgcgctg taccacttcc cggcgcgggc catccatttg caacaattgt gcaagaggac
     8581 ggttatcgag gcgtgcatgt cgataacgca gagactcaac accctgttta ttgatgctga
     8641 tgaatgacag ctcgtaattc agtgactgac tggccaggtt catctgctgt aataacgccc
     8701 cggacgcggg agtggccgag gcgttagcag agaataacag gctacctgtc actaatgaca
     8761 tggcaaacca aagttgcttc attactgcga ttgcgttcct aaagtttgaa ttcctggcac
     8821 ctgtacagcg gcttgctggg tttgcgcctg ctcaaactga agctgttcag agtggagtcg
     8881 gcgttgcagt tcgtaatcct gcaacattgc attaatgcga cgacgctgct cctgtacctg
     8941 ctgctgttga ccattatttg cggtcgcttc agaaggtact cccaggctaa ccgggctggc
     9001 tttacccatc atcggcagtg tattaaatac cggtgtttcg ggctgctggg acgtttcaga
     9061 ttgtccatta tagtgctgga cgccaacgat aactgcaagc gatacgcatg cggctacgcc
     9121 catttgggta agctgtgccg cccacggacg tactttctgc cagaatggca ttttctgcca
     9181 ttgatgcggc gcaggctggg cttccgggat caatgtcgcc ggttgacgta ctggctcttc
     9241 ttcaatggcg gccatcacgc gtgaagagat atcgaaatgg agcacctcgg gagtatcacc
     9301 ccgcattgag tcacggatta agtgatagct ttcccaggtt ttctgcattt ctgggttatg
     9361 agccagttcg ttaagcagct cactatccag cgtttcgcca tccattaaag cggaaagttg
     9421 ttctttctgc atgcctaata cccttatcca gtatcccgct atcgtcaacg cctgataagc
     9481 ggttgaactt tgttatcaat agcttccctc gctcggaaga tacgtgaacg caccgtacct
     9541 accggacaat ccatgatagc ggctatctct tcatagctca ggccatccag ctcccgcaag
     9601 gttattgcca tgcgtaaatc ttccgggagg gactcaatag ttcggaaaac tatctgtctc
     9661 agttcttctg acaacattaa gttctcaggg ttcgaaattt ctttcaacgc gccgccactt
     9721 tcgaagtttt cagcttcaat ggcatccaca tcactggaag gtggacgacg cccctgagca
     9781 accaggtaat ttttcgctgt atttacagca atccgataca gccatgtata aaaagcgcta
     9841 tctccccgga acgaatccag cgcacgatag gctttaataa aagcttcttg taccacatcg
     9901 ggaacatcac ccgacggcac atagcgggaa accagactcg ccactttatg ctgatagcgc
     9961 actaccagta agttaaaggc tttctgatct cccttctgga cccgttcaac caggacctgg
    10021 tccgttaact gctcgctcat ccgaggtaaa gtctccccaa accaaatttc cacgcgctat
    10081 cgaaacgcca ctccattagc tgcaatttga gcaagcaaag ggttagagtg tctcgttttt
    10141 gtaaagttcc gtaacgcatc tgtttttgtt tgtcatgctg tagacggatc attatctatc
    10201 attataagtc tacagaatct gaacatcgca ttatctgtgt agaaatgccc atttaactgc
    10261 ctgaagagta acccaacggc ctttttattt caccacctaa tcctccacca gccagtaact
    10321 tctctttttc tcgccgccct gcgtcagcgt gtttagccac tgtaacaaat attaaaatag
    10381 caggtgttta ttcgcacaac atgatgctat gctgaccaaa ccgtgtttag taaattaaac
    10441 aaagaaaatg aatactctcc ctgaacattc atgtgacgtg ttgattatcg gtagcggcgc
    10501 agccgggctt tcactggcgc tacgcctggc tgaccagcat caggtcatcg ttctaagtaa
    10561 aggcccggta acggaaggtt caacatttta tgcccagggc ggtattgccg ccgtgtttga
    10621 tgaaactgac agcattgact cgcatgtgga agacacattg attgccgggg ctggtatttg
    10681 cgatcgccat gcagttgaat ttgtcgccag caatgcacga tcctgtgtgc aatggctaat
    10741 cgaccagggg gtgttatttg atacccacat tcagccgaat ggcgaagaaa gctaccatct
    10801 gacccgtgaa ggtggacata gtcaccgtcg tattcttcat gccgccgacg caactggtag
    10861 agaagtagaa accacgctgg tgagcaaggc gctgaaccat ccgaatattc gcgtgctgga
    10921 gcgcaacaac gcggttgatc tgattatttc tgacaaaatt ggcctgccgg gcacgcgacg
    10981 ggttgttggc gcgtgggtat ggaaccgaaa taaagaaaca gtggaaacct gccacgcaaa
    11041 agcggtggta ctggcaaccg gcggtgcgtc gaaggtttat cagtacacca ccaatccgga
    11101 tatttcttct ggcgatggca ttgctatggc gtggcgcgca ggctgccggg ttgcaaatct
    11161 cgaatttaat cagttccacc ctaccgcgct gtatcaccca caggcacgca atttcctgtt
    11221 aacggaagca ctgcgcggcg aaggcgctta tctcaagcgc ccggatggca cgcgttttat
    11281 gcccgatttt gatgtgcacg gcgaactggc cccgcgcgat attgtcgccc gcgccattga
    11341 ccatgaaatg aaacgcctcg gcgcggattg tatgttcctt gatatcagcc ataagcccgc
    11401 cgattttatt cgccagcatt tcccgatgat ttatgaaaaa ttgctcgggc tggggattga
    11461 tctcacacaa gaaccggtac cgattgtgcc tgctgcacat tatacctgcg gtggtgtaat
    11521 ggttgatgat catgggcgta cggacgtcga gggtttgtat gccattggcg aggtgagtta
    11581 taccggctta cacggcgcta accgcatggc ctcgaattca ttgctggagt gtctggtcta
    11641 tggctggtcg gcggcagaag acatcaccag acgtatgcct tatgcccacg gcgtcagtac
    11701 gttaccaccg tgggatgaaa gccgcgttga gaaccctgac gaacgggtag taattcagca
    11761 taactggcac gagctacgtc tgtttatgtg ggattacgtt ggcattgtgc gcacaacgaa
    11821 gcgcctggaa cgtgccctgc ggcggataac catgctccaa caagaaatag acgaatatta
    11881 cgcccatttc cgcgtctcaa ataatttgct ggagctgcgt aatctggtac aggttgccga
    11941 gttgattgtt cgctgtgcga tgatgcgcaa ggagagtcgt ggcctgcatt ttacgctgga
    12001 ttacccggaa ctgctcacgc attccggtcc atcgatcctt tctcccggca atcattacat
    12061 aaacagataa aaagcctggg ttagcgccgt atacgcttcg gaatagttct ggtctggccc
    12121 acgaatgact aagcgatcgc taaagcattc tcccgcctgc ggggagaatg ccagcagcac
    12181 ccgatgcggc agtcgcgctt cgttttccgc cacatccgtc cgcaaacgta aatgccagcc
    12241 catacttaat gccagttcag taaaaccatt accaatctgc tctggcagca ccacgcagaa
    12301 aaatccctct tcggtaatgc actccgccgc acaggtcagc aacgatgggt gatcaagcgt
    12361 agtagtatag cgagcctgtt cccgttgagg tgtcgagcac tctactccct gctgatagta
    12421 aggtgggtta ctgatgatta aatcgaagcg tactgtctgc tgtgtgatcc actgctgaat
    12481 atccgccgta tggacgttaa tccgctctgc ccacggggac tggttgatat tttcctgcgc
    12541 ctgcgcggca gcttcacttt ccagttcaac tgcatcaatc atcacgctgt catcggttcg
    12601 ctgcgccagc attaatgcca gcaacccgct ccccgcgccg atatcaaggc aacgttttac
    12661 cccagccacc ggtgcccatg cgcccaataa aataccatcc gttcccactt tcatcgcaca
    12721 gcgatcgtga gcaacaaaaa actgtttaaa agtaaatcca ttacgacgaa gcacggatgt
    12781 agactgtgac atgaaaataa aaccttgcag gaaaaacggc gatagcaccg ggtgagaaca
    12841 atacccgaga agcgatatcc atacaaacag atgaagattg cagccgtaac gtctataatc
    12901 agcgccccac acagaggtag aacatgactg taacgacttt ttccgaactt gaactcgacg
    12961 aaagcctgct ggaagccctc caggataaag gtttcactcg cccgaccgcc attcaggctg
    13021 ccgccattcc gcctgcgctc gatggccgtg atgtactcgg ttctgcgccg acaggcaccg
    13081 gtaaaacggc ggcgtatctg ctgccagcgt tgcagcacct gctcgatttc ccgcgtaaga
    13141 aatccggtcc gccgcgtatt ttgatcctca ccccaactcg cgagctggcg atgcaggtgg
    13201 ccgatcatgc ccgcgaactg gcgaaacata cgcatctgga tatcgccacc atcaccggcg
    13261 gcgtagccta tatgaaccac gcggaagtgt tcagcgaaaa tcaggacatc gtggtcgcca
    13321 cgaccggacg tctgctgcaa tacataaaag aagagaactt cgattgccgc gcggttgaaa
    13381 cgctgatcct cgacgaagca gaccgtatgc tggatatggg cttcgcccag gatattgaac
    13441 atattgctgg cgaaacgcgc tggcgtaaac agaccctgct cttttcggca acgctggaag
    13501 gcgatgcgat tcaagacttt gccgagcgtc tgctggaaga tccggtggaa gtttctgcta
    13561 atccttccac tcgtgagcgc aaaaaaattc atcagtggta ttaccgcgcc gatgacctgg
    13621 aacataaaac cgcattgctg gtgcatctgt taaaacagcc ggaagcgacc cgctcaattg
    13681 tgtttgtgcg taagcgtgag cgtgtgcatg agctggcaaa ctggctgcgc gaagcgggca
    13741 tcaacaactg ctatctcgaa ggtgagatgg tacaaggcaa gcgtaacgaa gcgatcaagc
    13801 gtttgaccga aggtcgcgta aacgtactgg ttgcaaccga tgttgctgcg cgcggtatcg
    13861 acattcctga cgtcagccac gtctttaact tcgatatgcc gcgcagtggc gatacttatt
    13921 tgcaccgtat cggacgtacc gcgcgcgccg gtcgtaaagg caccgcaatt tcgctggtag
    13981 aagcccatga ccatctgctg ctgggcaaag ttggtcgcta tattgaagag ccaattaaag
    14041 ctcgcgtaat tgatgagtta cgcccgaaaa cgcgtgcacc aagcgagaag cagaccggca
    14101 agccatcgaa gaaagtactg gcaaaacgcg ccgagaagaa aaaagctaaa gagaaagaga
    14161 agccgcgggt gaaaaaacgc catcgcgata ccaaaaatat tggtaagcgc cgtaaaccaa
    14221 gcggaacggg cgtgccaccg caaacgacag aagagtaatc tcaatgccgg gtttaagccc
    14281 ggcattattt ttatcccggc aaaaaacaat cttcaacaca ctgaataaaa gtgtgcatcg
    14341 ccgggctaac cgcttttccg gcatggtgag cgcacattgc cgtaatggtc tgcgagtgtt
    14401 cgccaaaagg caattcaatt aattctccgc tctcaagctc tttttccacc gcaaagcggg
    14461 gcaagtaact gacaccgata ttcgccgcga cacaacgctt gatactttca atgcttaaca
    14521 gctcaatggt gttttccacc gtgatccgtc gctggcgcag cgtgctctca aatatctgcc
    14581 ggaagacaca ttgcggttcg ttgataataa agctacaggc gttatgtctt cccggctcag
    14641 taaaatcgac atctgcaatt tgtggtgaag ccaccagcac cagcgattgt tcacccaact
    14701 ctcgtcggtt cagggcatca tcattcccta cgcgataaaa gacgccgaca tcggcctcat
    14761 cattcagtag cgcatcacga atcacgtaac agttcagcga ctgcaacgat aaacgcactt
    14821 ttggtgcgcg ttgccgaaaa cgctgcaaca cctgcggcat tcggtaagag agtaacgttt
    14881 cacccgaaac aacgcgtagt tccccgtccg gatccgactc ctttttggca gcctcacgaa
    14941 gggtatccat cactctggta agttcataga tgtgcggcag cagctttttc ccttcgcggg
    15001 taaggcacat tcgtcgacca attttctcaa ataactgtac tgagaactcc tgctcaagct
    15061 gctgaatatg aaaagtcact gtcgattgtg tgcagcacaa tttttgcgaa gctcgcaaaa
    15121 aggaaccctc ttccaccacg gttttaagcg taataaaacg gcgcagatcc ataaccccaa
    15181 acctatcgaa aatatcgaat ttagaatgta aaaacattca tttttttaaa tattccgtgt
    15241 cgggtactgt ctaccaaaac agaggagata acaagtgaca ccgacgcttt taagtgcttt
    15301 ttggacctac accctgatta ccgccctgac gccaggaccg aacaatattc tcgctcttag
    15361 ctcagccact acgcacggct ttcgtcagag tacccgcgta ctggcaggga tgagtctggg
    15421 atttttgatt gtcatgctgc tgtgtgcagg catttcgtat tcactggcag tgattgatcc
    15481 ggcagcggta caccttttga gttgggcagg agcggcatac attgtctggc tggcatggaa
    15541 aatcgctacc agtccaacaa aggaagacgg gcttcagaca aaaccgatca gtttttgggc
    15601 cagctttgct ttacagtttg tgaacgtcaa aatcattttg tacggcgtta cggcactttc
    15661 gacgtttgtc ctaccgcaaa cgcaggcgtt aagctgggta gttggcgtca gcgttttgct
    15721 ggcgatgatt gggacgtttg gtaatgtgtg ctgggcgctg gcggggcatc tgtttcagcg
    15781 attgtttcgc cagtatggtc gccagttaaa tatcgtgctc gcgctgctgc tgatctattg
    15841 cgcggtacgc attttctatt aattaaaaaa gcggaagaag tcgccctctt ccgcttagta
    15901 acttgctact taagccttac aggctttcag taaaggtacg agcaataacg tcgcgctgct
    15961 gttccggagt cagagagtta aagcgaactg catatccgga tacacggatg gtcagctgcg
    16021 gatatttttc cggatgctta actgcatctt ccagagtttc acgacgcaga acgttaacgt
    16081 tcaggtgttg gccaccttca acgcgaactt ctggtttcac ttctactgga acttcacggt
    16141 actcaatatc acccagtttg cttactgcaa ccacttcgtc ttctgcaaaa cctgctttcg
    16201 caacgatgca acgcgcttcg cctttttcgc tgtccagcag ccagaaagag ttcagcagat
    16261 cgtcgttagc ggctttagta atctggatac ctgtaatcat gtgatgcctc cccggcaaaa
    16321 ttatttgatt tgttcagcct gtcgcggcca attggtaaaa ccattgttgc ttgagtgtat
    16381 atatactcct caaacaccct tgaatctttg atttaaatca ataaaaacca cacatcaagt
    16441 gtggtcgcaa atggatttta ttgttttaca tcaacttatg cgggtgtgaa attttaccaa
    16501 tttacatttt tttgcactcg tttaagtcta aaaaatgagc atgattttgt tctgtagaaa
    16561 gaagcagtta agctaggcgg attgaagatt cgcaggagag cgagatggct aacgaattaa
    16621 cctggcatga cgtgctggct gaagagaagc agcaacccta ttttcttaat acccttcaga
    16681 ccgtcgccag cgagcggcag tccggcgtca ctatctatcc accacaaaaa gatgtcttta
    16741 acgcgttccg ctttaccgag ttgggtgacg ttaaagtggt gattctcggc caggatcctt
    16801 atcacggacc gggacaggcg catgggctgg cattttccgt tcgccccgga atcgccactc
    16861 ctccgtcact gctgaatatg tataaagagc tggaaaatac tattccgggc ttcacccgcc
    16921 ctaatcatgg ttatcttgaa agctgggcgc gtcagggcgt tctgctactc aatactgtgt
    16981 tgacggtacg cgcaggtcag gcgcattccc acgccagcct cggctgggaa accttcaccg
    17041 ataaagtaat cagcctgatt aaccagcatc gcgaaggcgt ggtgtttttg ttgtggggat
    17101 cgcatgcgca aaagaaaggg gcgattatag ataagcaacg ccatcacgta ctgaaagcac
    17161 cgcatccgtc accgctttct gcgcatcgtg gattctttgg ctgtaaccat tttgtgctgg
    17221 caaatcagtg gctggaacaa catggcgaga cgccgattga ctggatgcca gtattaccgg
    17281 cagagagtga gtaaatttgc gggaaaatgc cggatggcag agttgccacc cggctgattt
    17341 atcaggcttt attctgacgc caccattcac caagcaaaac gcctgttgca acagagatat
    17401 tcagcccggc aacgttgccc gtaccgtcaa tcttcacgcg cagatcgttc ggatcgcgtg
    17461 cggcatccgg taacccttca tattcctgac ccagcaccag taccattttc gctggcaggc
    17521 tggttttgaa cagcggttta ccctgctcgc tggaagttgt cactacggtg taacccgcct
    17581 gacggaaatc atccagcacg ttaacaatgt tgtcgccagt aatcggctga acgtgttctg
    17641 cgccgccttc tgcggtacgg atagccgcac ccgactccag cagtgccgca tcctgcacca
    17701 caacgccttt cacgccgaag tgcgcgcagc tgcgcatcat gccacccagg ttatgcgggt
    17761 tagattcgtt ttccagtgcc agaacgcaat cctgcgcacc tgcctggctt acccactgct
    17821 gcacggttgt gccattacgc tttttgatca ggaagcaaac gccgccgtga tgttccgtgc
    17881 ctgacgcttt tgtcaattcc gcttcatcca ccacatggta cgctttgcgg tttgccgcca
    17941 tccagcgcaa ggcttcttta aaacgtggcg ttacgctctg gataaaccag gcgcgaacaa
    18001 tcgcttccgg acggctctgg aacagtgcct gacatgcatt ttcgccgtag acgcgggttt
    18061 cttccgcacg ctgacgacgc aacacttccg gatcaataaa acttttacca ctgataccac
    18121 cgtgatcggc cttttccggc gtctcatcac ccggcgcgcg ggaaaccgtg cgccacggcg
    18181 actcttccca cttgcggtca cggggctgac tctgtttgtc atcgcgggcg gggcgacggc
    18241 caccgtcagc acgagatttt cctggacgcc cgcccccttt cccggtacgc gggttgtggg
    18301 tacgtttatc agaatcatca tcactgcgga catacatcac tttgaccttg ccgcttttac
    18361 ctttcatttc atcgttcatg cttttctcca ccagcgctgc gcgaagcgcg cagattaccc
    18421 gaagtccgcg cggttcgcca tgatttcgta ccaaagcctg cgactatcat acctattgaa
    18481 taaaacagat tgttgtctgg aacaatgtcc ccgataatat gtaacatatt agaaacatac
    18541 cggcgtcgtt gccgataagt ctccttactc atcccgaggt tagttatgaa taccgtttgt
    18601 acccattgtc aggccatcaa tcgcattccc gacgatcgga tcgaagatgc ggcaaaatgc
    18661 ggacgctgcg gtcacgactt gtttgacgga gaggtgatta atgcgaccgg tgaaacgctc
    18721 gacaaattgc tgaaagatga tctaccagtg gtgatcgact tctgggcacc gtggtgcggc
    18781 ccctgccgta atttcgcacc aatttttgaa gatgtcgcgc aagagcgtag cggtaaagtg
    18841 cgctttgtga aagtgaatac cgaagctgaa cgtgaattaa gcagccgctt tggtattcgc
    18901 agtattccga cgatcatgat tttcaaaaac ggtcaggttg tcgacatgct taatggcgca
    18961 gtgccgaaag cgccgttcga cagctggttg aacgaatctc tttaatctta ccggggcgca
    19021 tcttgtgccc cgttttctcc tctgcgacaa tggcgttttt tcgacgctct cttatgaccg
    19081 aaaacgctgt tctccagtta cgcgccgaac gtattgcgcg cgcaacacgt ccttttcttg
    19141 cccgcggtaa tcgcgttcgt cgctgccaac gctgtctttt gccagagaaa ttatgtctct
    19201 gttcaacaat cacgccagcg gaagcaaaaa gtcgcttctg tttgctgatg ttcgacaccg
    19261 agccaatgaa gcccagtaat accgggcgtc tgattgctga tattttgcct gataccgttg
    19321 cgtttcaatg gtcgcgtacc gaaccctcgc aggatttgct ggatctggta caaaacccgg
    19381 attatcagcc aatggtggtc tttcccgcct cgtatgctga tgagcaacgg gaagtgatct
    19441 tcacaccacc tgccggtaag ccaccgctgt ttatcatgct cgatggtacc tggccggaag
    19501 ctcgcaagat gtttcgtaaa agtccgtatc tggataatct tcccgtcatt tccgtcgatc
    19561 tttcccggct ttctgcctat cgcctgcgtg aagcccaggc tgaaggccaa tattgtactg
    19621 ccgaggtagc catcgcactg ttagatatgg ccggcgatac cggggcggca gcagggttag
    19681 gcgagcattt tacccgcttt aaaacacgct atctggcagg aaaaacgcaa catctgggta
    19741 gcatcacagc agaacagtta gaaagcgttt aaaatcattc ggtcacttct gcgggagacc
    19801 ggtatgagtc agcgaggact ggaagcacta ctgcgaccaa aatcgatagc ggtaattggc
    19861 gcgtcgatga aacccaatcg cgcaggttac ctgatgatgc gtaacctgct ggcgggaggc
    19921 tttaacggac cggtactccc ggtgacgcca gcctggaaag cggtgttggg tgtgttggcc
    19981 tggccggata ttgccagctt gccctttaca cccgaccttg cggttttatg taccaatgcc
    20041 agccgtaatc ttgctcttct ggaagagctc ggcgagaaag gctgtaaaac ctgcattatt
    20101 ctttccgccc cggcatcgca acacgaagat ctccgcgcct gcgccctgcg ccataacatg
    20161 cgcctgcttg gaccaaacag tctgggttta ctggctccct ggcaaggtct gaatgccagc
    20221 ttttcgccgg tacctattaa acgcggcaag ctggcgttta tttcacaatc ggctgccgtc
    20281 tccaacacca tcctcgactg ggcgcaacag cgtgagatgg gcttttccta ctttattgcg
    20341 ctcggcgaca gcctggatat cgacgttgat gaattgcttg actatctggc acgcgacagt
    20401 aaaaccagcg ccatcctgct ctatctcgaa cagttaagcg acgcgcgacg ctttgtttcg
    20461 gcggcccgta gtgcctcgcg taataaaccg attctggtga ttaaaagcgg acgtagcccg
    20521 gcggcacagc gactgctcaa cacgacggca ggaatggacc cggcatggga tgcggctatt
    20581 cagcgtgccg gtttgttgcg ggtacaggac acccacgaac tgttttcggc ggtagaaacc
    20641 cttagccata tgcgcccgct gcgtggcgac cggttgatga ttatcagcaa cggtgctgcg
    20701 cctgccgcgc tggcgctgga tgccttatgg tcacgcaatg gcaagctggc aacactaagc
    20761 gaagaaacct gccagaaact gcgcgatgca ctgccaggac atgtggcaat ctctaacccg
    20821 ctcgatctgc gcgatgacgc cagcagcgag cattatgtca aaacgctgga cattctgctc
    20881 catagccagg attttgatgc gctgatggtt attcattcgc ccagcgccgc tgctcccgca
    20941 acagaaagtg cgcaagcatt aattgaagcg gtaaagcatc atccccgcag caagtatgtt
    21001 tctctgctga cgaactggtg cggcgagcac tcctcgcaag aggcacgacg tttattcagc
    21061 gaagccgggc tgccgaccta ccgtaccccg gaaggaacca tcactgcttt tatgcatatg
    21121 gtggagtacc ggcgtaatca gaagcaacta cgcgaaacgc cggcgttgcc cagcaatctg
    21181 acttccaata ccgcaaaagc gcatcttcta ttgcaacagg cgattgccga aggggctacg
    21241 tcgctcgata cccatgaagt tcagcccatc ctgcaagcgt atggcatgaa cacgctccct
    21301 acctggattg ccagtgatag caccgaagcg gtgcatattg ccgaacagat tggttatccg
    21361 gtggcgctga aattgcgttc gccggatatt ccacataaat cggaagttca gggcgtcatg
    21421 ctgtacctgc gtacagccaa tgaagtccag caagcggcaa acgctatttt cgatcgcgta
    21481 aaaatgacct ggccgcaggc gcgggtccac ggcctgttgg tgcaaagtat ggctaaccgt
    21541 gctggcgctc aggagttgcg ggttgtggtt gagcacgatc cggtttttgg gccgttgatc
    21601 atgctgggtg aaggcggtgt ggagtggcgt cctgaagatc aagccgtcgt cgcactgccg
    21661 ccgctgaata tgaacctggc ccgctatctg gttattcagg ggatcaaaag taaaaagatt
    21721 cgtgcgcgca gtgcgctacg cccattggat gttgcaggct tgagccagct tctggtgcag
    21781 gtttccaact tgattgtcga ttgcccggaa attcagcgtc tggatattca tcctttgctg
    21841 gcttctggca gtgaatttac cgcgctggat gtcacgctgg atatcgcgcc gtttgaaggc
    21901 gataacgaga gtcggctggc agtgcgccct tatccgcatc agctggaaga atgggtagaa
    21961 ttgaaaaacg gtgaacgctg cttgttccgc ccgattttgc cagaagatga gccacaactt
    22021 cagcaattca tttcgcgagt caccaaagaa gatctttatt accgctactt tagcgagatc
    22081 aacgaattta cccatgaaga tttagccaac atgacgcaga tcgactacga tcgggaaatg
    22141 gcgtttgtag cggtacgacg tattgatcaa acggaagaga tcctcggcgt cacgcgtgcg
    22201 atctccgacc ctgataacat cgatgccgaa tttgccgtgc tggttcgctc ggatctcaaa
    22261 gggttaggct taggtcgacg cttaatggaa aagttgatta cctatacgcg agatcacgga
    22321 ctacaacgtc tgaatggtat tacgatgcca aacaatcgtg gcatggtggc gctggcccgc
    22381 aagctcgggt ttaacgttga tatccagctc gaagagggga tcgttgggct tacgctaaat
    22441 cttgcccagc gcgaggaatc atgagtaagg tactggaaat gttgaccact taatcgggac
    22501 tggtgttatt attgcccgct tatgtcgtct gcattgcaca gaggaccctt caatgaacag
    22561 agaagaaatg cactgtgatg ttgtcaaaat ttaagcgtaa taaacatcaa caacaccttg
    22621 cccaactacc caagatttct caatcagttg atgatgtcga tttcttttac gctcccgccg
    22681 acttccggga gacgctgctg gaaaaaatag ccagcgcgaa gcagcgcatt tgcattgtcg
    22741 ccctgtatct cgaacaggat gacggtggca aaggcattct gaacgcgttg tatgaagcta
    22801 aaaggcagcg tccggaactg gatgtgcggg tgctggtcga ctggcatcgt gcacaacgtg
    22861 gacgcattgg cgctgcggca tctaacacta acgctgactg gtactgccgc atggcgcagg
    22921 aaaatccggg cgtagatgtt ccagtttatg gcgttccaat caatacccgt gaagcccttg
    22981 gcgttctgca ctttaaaggc tttatcatcg acgatagcgt actttatagc ggtgccagcc
    23041 tgaacgatgt ttacctgcat cagcacgata aatatcgcta cgaccgttat caccttatcc
    23101 gtaaccgtaa gatgtcagac attatgtttg aatgggttac acagaatatt atgaatggcc
    23161 gcggcgttaa tcgtctggat gatgttaatc ggccaaaaag cccggaaatc aagaacgata
    23221 ttcgtctgtt ccgccaggag ctgcgtgatg ccgcttatca tttccagggc gatgccgaca
    23281 acgatcagct ttccgtaacg ccgctagtgg ggctggggaa atcgagtctg ttgaacaaga
    23341 ccattttcca tcttatgcct tgtgccgagc agaaactaac catctgtacg ccatacttca
    23401 acctgccagc aatccttgtg cgcaatatta tccagttgct gcgcgaaggg aaaaaggtcg
    23461 aaattattgt tggtgataaa accgcgaacg acttctacat tccggaagat gaacctttca
    23521 agataattgg cgcattgcct tatctctatg agatcaatct gcgtcgtttc ctgagccgtt
    23581 tgcagtatta cgtcaatact gaccagctag tggttcggtt atggaaagat gacgacaaca
    23641 cctatcacct aaaagggatg tgggttgatg ataagtggat gttgatcacc ggtaataacc
    23701 tgaacccgcg cgcctggcgt ctggatctgg aaaacgccat tttgatccac gatccacaac
    23761 tggagcttgc gccgcagcga gagaaagaac tggagctgat ccgcgagcat accaccatcg
    23821 ttaagcacta tcgcgatctg caaagtattg ccgattatcc ggtgaaggtt cgtaaactca
    23881 tccgccgttt gcgccgtatc cgcatcgacc gattaattag ccgcatcctg taatcacaac
    23941 cccgtcctgt acggggtttg ttttttggag gccacgtttt gcgtattctt tttgtctgtt
    24001 cgctattgtt actttctgga tgcagccata tggctaacga tagctggagc gggcaggata
    24061 aagctcaaca ctttatcgcc tcggcgatgc tttccgccgc cggaaatgaa tattcacagc
    24121 atcaggggat gagccgggat cgcagtgcca tgtttggatt gatgttctct gtcagtttgg
    24181 gggcgtcaaa agagctttgg gatagccgcc ccgaagggag cggctggagc tggaaggatt
    24241 tggcctggga tgtcgccggt gcaagcaccg gctataccgt ctggcaactg acccgtcact
    24301 aaagacgcat ccccttccct ttgcgatgta gcatcaaaga aaccagaaac gccaccacgg
    24361 ccatcaaggt cacataccag aagaaggctg tttccattcc tattgatttc agcgacaacg
    24421 ctacgtactc cgccgaacca ccaaatatag cattagcgac cgcatatgac agaccaacgc
    24481 ctaatgcgcg aacctgtgcc gggaacatct cagccttcag tattccactg attgatgtat
    24541 aaaaactcac tatcagcagg gcacacatca ccagaccaaa agcggcataa ggcgaggaaa
    24601 cgttttgcaa tgctgagaga ataggaacgg taaaaatggc tgccagtgaa ccgaaacata
    24661 acattgaggt acggcgacca atcttatccg acagcgcgcc aatgagtggt tgaataagca
    24721 tgaatacaaa caatgcggca gtcataatgc cactcgccac gttggcatgc attcccgcag
    24781 tatttaccag atacttctgc atataagtgg tgaaggtata gaaacaaagg gagcccgcag
    24841 cggtaaaacc gagaaccatg atgaatgcac ggcgattgcg ccataatcct ttcagagatc
    24901 cagcttcttt taaagcgcgc gtttcttgtt gcgaagtttc atctaactga cgacgtaacc
    24961 acaacgccac aacagctaac acagctccta acgcgaaagg aatacgccat ccccactctc
    25021 tgagtgcagc gtcttccatg gtgtgttgta aaaccacgac aaccagtagg gctagcagtt
    25081 gtccgccgat caacgtcaca tactgaaatg atgcgtaaaa acctttgcgc ccttcaacgg
    25141 caacttcact catataggtg gcgctggtgc catattctcc gccaacggat aatccctgaa
    25201 ataaacgcgc gagaagcaat aatgccggag cccacgtacc gattgtttca taaccaggca
    25261 aacaggcgat taccagtgag ccgaaacaca tcatacacac cgatagcagc atcgattttt
    25321 tgcgaccatg tttatcggct atgcggccaa atagccaacc gcctattggg cgcatgagga
    25381 atcccgcagc aaaaacacct gctgtttgta gtagttgagt cgtcgtgttc ccggaaggga
    25441 agaagatgtg ggcaaagtag agtgaacaga acgagtagac atagaaatcg aaccactcga
    25501 ccagattacc tgaagaggcc cccacaatcg cccaaatgcg gcgacgtgta tcactacttg
    25561 tcagtttgct gtctgccgtt acagtacttt cagccatgcc attatgtctc ctgccgtaat
    25621 ccgatgcttt tgtcggtcgc ttttgtttat ttcttttggt aaagaaaatg ttatacattt
    25681 gttgcgtgtc attatgcaac cttaaccatg aatttagtta gcaggaaaat ggttattgag
    25741 gagcttaagg ataaatttct ggtgaggaga acacgtatgg aggtgggcaa gttggggaag
    25801 ccgtattcgc tgctgaatct ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg
    25861 catttttttc accaacgcaa aaaggccatc cgtcaggatg gcct
//
LOCUS       DAZGCQ010000070        25409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000070 DAZGCQ010000000
VERSION     DAZGCQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.070"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1138..2184)
                     /locus_tag="ACHK07_003183"
     CDS             complement(1138..2184)
                     /locus_tag="ACHK07_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8103479.1"
                     /translation="MSKRKNYTGKRGQHSLPDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFIPHSLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSGLDTYWYLNDDGTEFSFRKG
                     AVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGIEVM
                     RFNPYALLEEGSAR"
     gene            complement(2190..3923)
                     /locus_tag="ACHK07_003184"
     CDS             complement(2190..3923)
                     /locus_tag="ACHK07_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO8103480.1"
                     /translation="MIQDAFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDDW
                     DATPPIQRVTQSIDARLIQLTAKSDKTGGDFKEIDLLTRQLKKLNEGQGEQAVSGKKP
                     RKRKLKNHFTEEQIIALRERTLRTLACHQREWYENRHHRNRMILKSRQIGATWYFAHE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVQLRKVAGAMATLKGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSERPRSKRIEFDVSHSNLKGGVLFPDKTWRQIVTIEDAVGNGWEY
                     TSIDDIRDENTESEFRNLYMCEFMRDGEAAFSLNALIGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPMVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVFQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            4080..4916
                     /locus_tag="ACHK07_003185"
     CDS             4080..4916
                     /locus_tag="ACHK07_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8103481.1"
                     /translation="MGSQKAPERKKFRVAVSGSTIDGREISGEMLRRAAENYDPSVYG
                     SRVNIEHALSLWPSSELCAMGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMLNE
                     GKKVYSSIELLPDLDAVQGPYIVGLAMTDTPASLGTERLKFAAQQRDSIMRFNNRTSE
                     PSMFTAAMEAELLTTAEQQTEEGRQWFSRVMSLISKARGTDSEQFSQIREVVESIASD
                     HADLLDRFSALEKKHASGQETLKKLHDELAELKTQLKTLDADPQQRFIATGGNNQILA
                     EF"
     gene            4934..6019
                     /locus_tag="ACHK07_003186"
     CDS             4934..6019
                     /locus_tag="ACHK07_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO8103482.1"
                     /translation="MARLSLSREGRDSLERYMLQQAQLAGITTERLSKTFSVDPSIQQ
                     KMENAIKESAELLQKINVIGVDNQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSTQKNFQQLLSAQIARQIALDRIMIGFNGESHAL
                     ISDRAANPLLQDVNPGWLKHIRDKADTRIVKGMTLTRRDEENKLVSKGDYGNPDAMVH
                     DTRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRAI
                     GGLPTYLAPFFPKDAMLITSLSNLSIYFQKGSLRRYIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGISFAGEDPDEKPDSEE"
     gene            6085..6876
                     /gene="gpM"
                     /locus_tag="ACHK07_003187"
     CDS             6085..6876
                     /gene="gpM"
                     /locus_tag="ACHK07_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO8103483.1"
                     /translation="MLTPAQRYVQQAVARNRVTMSTRETQRERTAHEQVLHQLRLAQQ
                     QLKGVQSNIARAELKRHLLPDFEGWIEGTLAGNSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLTEEIAEIVLTQAATNSDTDLSGYTVMLDTLHGL
                     VHDQDMPDQVRAKLNKARAFSRRASTQPDDIHQSLMLLREAMRLNPAAGVKRDIATLE
                     RAVKKLSSPPPDPVKKSAKTTRKTGNSQKTSAKKKPSTRKPKNTH"
     gene            6986..7480
                     /locus_tag="ACHK07_003188"
     CDS             6986..7480
                     /locus_tag="ACHK07_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8103484.1"
                     /translation="MSLIARTPAEHISEDIPDTDDGDSCVTADAFWPVIVLRELRLAV
                     RLPGRTTTARLMHAATEAVAHVTAELEMWKQERMKEGYTALADIPATHLNSESVHVHR
                     YRRAVYALARAFVLERTRDVDTTEKGDKKADSLDPQVEALWRDARWAISDIRGVTRIY
                     AELV"
     gene            7480..7680
                     /locus_tag="ACHK07_003189"
     CDS             7480..7680
                     /locus_tag="ACHK07_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO8103485.1"
                     /translation="MKIKALEGDTVDLLCFRHYGSTQGVTEQVLEANPGLCEQVFLVA
                     GQEVELPEQIREKKQETVLLWS"
     gene            7671..7967
                     /locus_tag="ACHK07_003190"
     CDS             7671..7967
                     /locus_tag="ACHK07_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103486.1"
                     /translation="MELNIFQRIYDHITFSMSLMVTGIGVMTVSEKIAVAGLLLGALS
                     GLRAWIHRARLEKAQRRRNELIAQILAQSESRPLNASEKQALKQLEDQDDAPPH"
     gene            7951..8496
                     /locus_tag="ACHK07_003191"
     CDS             7951..8496
                     /locus_tag="ACHK07_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8103487.1"
                     /translation="MPRHIKRYAVATIVALGVTLAPGALRTSEEAQVKIATWEDCRAR
                     PYYDTAGILTTGCGSTGNVKNRLYSENEVAERWVNDMQRAENCINKNFHGAAMPQSVF
                     EAMTDAAYNLGCTSLMWFKDRRGQRHRTTIWKHAQAHRWIQACARLTDFVNSGGKRIP
                     GLVNRRTDFKAWCLKDTGATS"
     gene            8493..8933
                     /locus_tag="ACHK07_003192"
     CDS             8493..8933
                     /locus_tag="ACHK07_003192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO8103488.1"
                     /translation="MKPAGILFLMVLCMVAIAIAFRQTWRLGQESARNEALHETIRSN
                     SATLESIIAISRNTHALIAGLKVAEQHRNQEGEVRREHIRNAIRSDRCADTPVPAAVA
                     DSLQKRTARLSPELVRSSTGEPHHSHNATTADGASDMGKNRSME"
     gene            9040..10938
                     /locus_tag="ACHK07_003193"
     CDS             9040..10938
                     /locus_tag="ACHK07_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8103489.1"
                     /translation="MKKYELITAMTCDELAKALDERLAEGWQPHGAPVAASEPGVPFY
                     LMQALIKSTDTATQGSNINTPTAEPEYYFVIPVAGQSNGMAYGEGLPLPETCDRPDPR
                     IKQLARRSTVTPGGESCRYNDIIPADHCLHDVQDMSGKNHPKADTSKGQYGTVGQGLH
                     IAKKLLPFLPSDAGILLVPCCRGGSAFTAGADGTYSDTTGASGNSARWGVDKPLYKDL
                     ISRTKAALAKTPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVEKFRTDLAEQAEQCT
                     GGSAAGVPWICGDTTYFWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGANVPTN
                     NPAEDPDIESIGYYGSTWRNSAATWTSADRESHFSSWARRGIISDRLATAILTHTVRA
                     AAVQPGASSSEPDVPETAATTTLLSYIASESEGNLKAQGWSASGGKAEIISDTGATGG
                     KAMKLTKQTGKGSWVLEHDAGTGDSLLQKGGLIRCRFKAQGELAANRYVMALYWPVSS
                     LPQNVTLAGNAGNNLLASFYIQTDAKDMNVMYHNAKAAKDNQKLGTFGAFDNEWHTLA
                     FRFAGNNSLQVTPVIDGKDGTPFTLTQSPVGIFTADKLHVTDVTKNATYPVLIDSIAV
                     EINNPVTA"
     gene            11091..11243
                     /locus_tag="ACHK07_003194"
     CDS             11091..11243
                     /locus_tag="ACHK07_003194"
                     /inference="COORDINATES: protein motif:HMM:NF018789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8103490.1"
                     /translation="MDPVYTILLCFCTITCVLFLASGGGEILYQWCAKKLNEWLNKSE
                     RDTPDQ"
     gene            11265..11735
                     /locus_tag="ACHK07_003195"
     CDS             11265..11735
                     /locus_tag="ACHK07_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103491.1"
                     /translation="MLKMNALRDTLADTCRWCKANPDKFTVCVESGSIETTGESPSFL
                     YRYNLVIFAMDFTQDIDELFLPLLHWLYHNQPDLLLNPEKNSSIKFSASPNNDDSVDV
                     VIELPVWERVRVTRTPDRQVRAEHLQEPRPRIPSARGAWSNVYEDVTWSIPRDE"
     gene            11728..12375
                     /locus_tag="ACHK07_003196"
     CDS             11728..12375
                     /locus_tag="ACHK07_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8103492.1"
                     /translation="MNEHKLDAVFRDILAGMSAAGLSRTARQVGQAVRRSQQQRMRAQ
                     KAPDDTPWAPRKRQIQRVQQGIKFLWQPPAAGSQPMVRELKNWKHGTGRHGPHITGYD
                     INRGGLRTFYRADIEEYLEIRTRRVSQGNTVRARMFERLRRYVKILQADNESIRVGFN
                     GDQARIARVHHFGLRDNVVPGVTTQYPARELLGLSVKDKAMIRETIIGSLKGGMK"
     gene            12372..12953
                     /locus_tag="ACHK07_003197"
     CDS             12372..12953
                     /locus_tag="ACHK07_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8103493.1"
                     /translation="MSAEIMRLLENMIRVGVISDVDLKNWRVRVTSGRLTTDWLRWNC
                     TRAGVFKIWLPPSRGEQVLLLCPGGNPEMAMVVGSLYSTDNPAPGTQQDEVIITARDG
                     ARLRYNAKEGALDATGIKTARVTAETRITLETPLVECTQHLKTRTFELTDGGTLKGDV
                     THSGGSLTSNGVVAHTHTHSGVQSGGSQTGGPQ"
     gene            12950..13300
                     /locus_tag="ACHK07_003198"
     CDS             12950..13300
                     /locus_tag="ACHK07_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO8103494.1"
                     /translation="MTATYTGMNPYHPGVVHDTEHLHCSVRDILLTPQGSRIMRRDYG
                     SLVPDLLDAPKNDVTRLQCMSAAVIALTRHEPRLALNTIDVRWLDEGGAELLLTGIIT
                     ATMQTVRLSIRTGD"
     gene            13304..14200
                     /locus_tag="ACHK07_003199"
     CDS             13304..14200
                     /locus_tag="ACHK07_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8103495.1"
                     /translation="MPTVDLSQLPKPAIINTPDFEVILNEIKTMMIGCFPEEQQPAVT
                     AALALESEPLTIIAEVMAFREMLLRQRINEGVAACMLSHASGTDLDHLAANLNTQRLV
                     ITPATDTAPTVMENDTALRLRAQSAYEGLSVAGPSASYEYFARSASGQVADARAVSPS
                     PAVVVVSILSTEGDGTASQELIQTVSNALNDERIRPVADRLTVQSAKIVKYRINARLF
                     LYPGPESEPVILAARQSLDEWLTEQGHIGRDVARSAIMSALHVQGVQRVELEEPASNI
                     AVDDSQFAHCESIIVEMGGTNE"
     gene            14193..14735
                     /locus_tag="ACHK07_003200"
     CDS             14193..14735
                     /locus_tag="ACHK07_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO8103496.1"
                     /translation="MSDSLLPPSASDFLKYTGYATDRITRIPVDLRTLWDPEACPVEL
                     LPYLAWALSVERWDKNWPEETKRKVIRESWLIHRHKGTFSSLRRIVEPLGYIISMSEW
                     WETGDPPGTFRLNVGVFEGGLTEEMYFEIERLIADARPVSRHLVGLNIVQDISGKVYT
                     GGMVYDGDIITVYPIQESDA"
     gene            14732..16387
                     /locus_tag="ACHK07_003201"
     CDS             14732..16387
                     /locus_tag="ACHK07_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103497.1"
                     /translation="MSDTVKFKTIITTAGAEKLAASASDGGKKVTPAVMAVGDGGGTL
                     PDPDAKQTALINEVWRHDLNRVFIDNTSKNTVVAELIIPAETGGFWMRELGLYDDEGT
                     LIAVANMAESYKPRIAEGTGRSQICRMVIAVSNIEIISLTVDSTVVTATKEYVDNLLE
                     EHEKSRNHPRASQAAPGLVQLSSQTDLDSEIMAATPKAIKIVMENANGRLAKNRNGGD
                     IPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWLNERGAFRPGLWIFKANISL
                     SRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLN
                     KGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPWPSDTPPGGYAIMVGQIFDK
                     ARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTK
                     TTSSFNYGTKTTNSAGPFSATISLRTATASPEGSGDWTAYGPGSGANLIARVSEHTHS
                     VSIGEHIHTLALGQHGHGLTINPTGNAENTVKNIAFNYIVRLA"
     gene            16387..17112
                     /locus_tag="ACHK07_003202"
     CDS             16387..17112
                     /locus_tag="ACHK07_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8103498.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWHLMEDHRGKTVYHEETGEKLVITGPGPLPSSVTTEPP
                     GTSADVYRDGQWVTDLSAARGIKNSEINDWRNRMENAGYIMEYNGRKWDYGKATQDRL
                     EPSVTAARAGKLPAEFFWTDAENNDVPMSADELIALSEAVGEAMFKKGLEIHIRQREM
                     KKELAGMDNAGEIMKFTPGWKTT"
     gene            complement(17155..17643)
                     /locus_tag="ACHK07_003203"
     CDS             complement(17155..17643)
                     /locus_tag="ACHK07_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103499.1"
                     /translation="MMMIYGMFVFQLSTLPHQQLQQSRGWRHVSNERINRSAGWQYIG
                     AGEDKITLSGVLYPEITGGEVSLTVLNTQAYTGRPWPLIDGAGQIYGMYVITDIQTTR
                     EEFDRYGRAKKITFSVSFRRCDEDLRERLQASSVGDLLNNLKDAASAAGERVSSMASG
                     IL"
     gene            complement(17666..20461)
                     /locus_tag="ACHK07_003204"
     CDS             complement(17666..20461)
                     /locus_tag="ACHK07_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8103500.1"
                     /translation="MNTLDIRVAFSAADNLTRPATSARESAEALAASLRNTQNNLRDL
                     ERQSRSFSSARKHLTEVTDKIKRAQRELNGLQRLQQSGRPLTEAQQELINKLTARLER
                     LNEVRTQERNRLREAGQALRRHGVTLSGGDRAIEAAIRRTQQYNEELERQRLALARVS
                     RARDEYTRAMAVSEKLRGAGTAMMLAGLGGMYAGSRMVAPVMNEERYGAVIAAQTGEG
                     RAEASGYTRLVRELHHAGIAGDAARAAEAVGAVRSTLGALGVTGDTEISRITRRMMDM
                     QTVMGVDVPQGIQAAAIMMKNGLARSSDEALDLMVSAMQRMGPEMRGELPEIIHEYST
                     HFRNLGFSGAEAMSLLADMAGQGKFALDKTGDAVKEFSIRGSDMSKSSVEAYESIGLN
                     AAQMSSAIASGGVAARDAMQKTAAGLLRIRDPAERANAAIALFGTPVEDLAVDQIPAF
                     LGALARTSVVLGNTEGAAERMGNTLRDNLSGDVLKLTGSLAGLRADAVSTVTETLRGL
                     VQTATQWTARMREWVQKNPELVRGLLMVAGVVAGLSAAIGGLLVTVGMVLIPLVKLRL
                     GLSLIRALPAGISVVSGLFGGLGGVISSLGTAVGMLTGPVGLVVAALLGAGLLIYKYW
                     DQVKAFVLGFIDGARGALAPLLATVSRFAPLLDLVAGAVSRVFLWFKSLLSPVAATRD
                     TLDKCTVAGARFGSSLVSALTLVFSPMKALIDGLAWVLEKLGVLPGEAARVKQKLDSA
                     VKTPVVWEWDPEQKKMVQKAWNWSPKGAGGTQSVGALRLPEMPAVLQPSANSGNGGVL
                     DRLKGIEGNTRDIVRETKKRTGPGDIIFRNLPPALVVRGQWTEPRLAGSTGVSGARIE
                     EHQNIKVASPPPLTVPSSGSERGLSSVGFSGEIHVHLHNVVTQDPRTLARMIGEAVKA
                     ELSGQSRNGLRSFRDVD"
     gene            complement(20458..20604)
                     /locus_tag="ACHK07_003205"
     CDS             complement(20458..20604)
                     /locus_tag="ACHK07_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO8103501.1"
                     /translation="MQFEHIEDVVADIAVVFNWSPADLFMMGPEDVIAWRKRASDRNG
                     SPEK"
     gene            complement(20613..20984)
                     /locus_tag="ACHK07_003206"
     CDS             complement(20613..20984)
                     /locus_tag="ACHK07_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003835883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO8103502.1"
                     /translation="MNENAKLTKTAELVTDKDIGSNASPDVISLITPVVRDTETIKSV
                     KIGPLARQAGSLRGLSLVDLYGMKFDALLAYLSRVTEPRLKESELKAMDARDFSALAN
                     AAVNFYVGGVSTKPNGEETDG"
     gene            complement(21026..21538)
                     /locus_tag="ACHK07_003207"
     CDS             complement(21026..21538)
                     /locus_tag="ACHK07_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8103503.1"
                     /translation="MAVPHKLRFMTLFIDGKNEIGQVVSVTLPKLTRKTESYRGGGML
                     GSVAVDLGLDDSALDASFVMGGAAADLVRKYAGRIDEVRLRFAGEYYTDGDSQLIEVE
                     MRGRISEIDMGEAKQGEDTQHTYNVKNTYYKLSSDDRELLEFDLLNFIYRQDGENIVP
                     DRIRSALGMG"
     gene            complement(21538..22725)
                     /locus_tag="ACHK07_003208"
     CDS             complement(21538..22725)
                     /locus_tag="ACHK07_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO8103504.1"
                     /translation="MSETRFHGARVRESTDLVTAINDIDSSVIGIVAVADDADTGTFP
                     LDKPVLLTRVNDVLGKAGKAGTLYKALKAISDQVSTKVIVVRVAAEKKGDKQKTQDQL
                     VIGAVEADGRYTGMQALLVAEQDEAIGYRPRILAAPGLDTKAVTTALCTIAGKLRAFV
                     YAGCPGCNTATEAMTWRQDLAYRELMLIWPDFIAYNPQSGKNESFPSAAYACGLRARL
                     DHEQGWHKSLSNEPVKNVLGVSHPVYWSLQSEDSDANNLNNKEITTIIRRNGFRFWGN
                     RTTDTSAYIFEVYTRTAQTLADTIAEAQFEVVDDPLTPANVKDAISAIRAKLNELVTA
                     GKLIGAECWYDVIDNSTTDLRQGRVRIRYKYTPVPPLEDLTLHQTFTDEFFGPAFASL
                     GGA"
     gene            22887..24014
                     /locus_tag="ACHK07_003209"
     CDS             22887..24014
                     /locus_tag="ACHK07_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO8103505.1"
                     /translation="MADRIIRIIKNYDDRTSTLVKLPDFSITLNNNELEAQELKNRVM
                     SLSVTDNRGFEADQISLTLDDTDGQLILPERGAVLSIAIGWKGESLTDKGTYIVDEVS
                     HDGPPDKLIIGASSADFREEFNVKREVSWHDVTLERVVSAIAHRYGLKAQISEMLMHI
                     EIDHADQTEESDMSFLTRMADMLGAIATVKNGNLLFIIPGGGVTASGKPLPSFTLTRS
                     DGDQHRFRIADRQAYTGVRAYWLDLNFGKKKQVSVKKRSLSQEKSHSREGDYMEGAEG
                     NVFVLRRTFPNEESAKRAAAAKWQQLQKGAAEFAITLAHGRADLYPEMHGTVSGFKTT
                     IDRQDWIIARVEHTIDSNGFTTQLELEAKIPDWIAESEQSG"
     gene            24058..24309
                     /locus_tag="ACHK07_003210"
     CDS             24058..24309
                     /locus_tag="ACHK07_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8103506.1"
                     /translation="MFYCPHCGASARIRTSKAANDTGTIRYKYYQCNNIECGATFSTM
                     ESVHKMISVPTPSGERDEQIPHEHFPATHRGRLQLILEL"
     gene            24495..24635
                     /locus_tag="ACHK07_003211"
     CDS             24495..24635
                     /locus_tag="ACHK07_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8103507.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHNSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            24964..25104
                     /locus_tag="ACHK07_003212"
     CDS             24964..25104
                     /locus_tag="ACHK07_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8103508.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSLWGAEFAAFLQC
                     KQ"
BASE COUNT         6648 a         7020 c         6451 g         5290 t
ORIGIN      
        1 ggaaggcagt taagtaattt gtcagcaacg ttaaaaatgc tgagtcgata cggctttgtc
       61 agcctggaaa aacaaggtaa ctgcgtacac ccgaaagcgt tgttcacgga ttttgaaatc
      121 ataatagacc cgtcagtagg tgaaactcat cgggcagcgt gaatattgcg ggggtaatcc
      181 ccgctcatac ttttttagcc tgcgtcacct gcctgaatac ccacctgaaa aaatgcacaa
      241 aagtgcacga aattgcacaa tttttcttat gtattttttc cccattcagc ccggtaacgg
      301 cgcgggctgg ggccagtttg cacggtgcac gaaaaatgag gcaattgcag cgcgcaggtg
      361 gcgtgggaca gggcgcacga attgaggggt ggaaggcaac ttgctttatt tggggccgta
      421 ttgcgcgtga tacgcacaca gtgaagccag ttttgaaaaa ccacatcaaa acatagccag
      481 agaaacagtc cgtcagaatg cgtctggaga ggtcggggaa gccagagaaa aagcgagcgt
      541 cgaacgtgat gaaaaagagg gataaaaatc accggaatca taaagttaag agcatttaaa
      601 gtatccagga tccacgggtg aaaacacacg cacaatatga ggtgaacgca gagtgcaggc
      661 gggcatcaac agaccgaagg aggacggtat gaaccatttt cgcgactggt gtcaggcaaa
      721 ccagaagagc caggacgcga gacgggaggc gctctgtatt ctccgccgcc agtccaggct
      781 gcaaagaaaa gcgcctccgc attccgttaa gagagctggt gagtcggggc ataaattttt
      841 tatgccccgg cgaagcagca gacaagcgaa gcgcgtcagt tggtattcgt actatccggc
      901 catcacccga acaggacgaa cgccacgacg tgttacacga gtaccggaga cagtctggtg
      961 attctggccc ttaatccggt aatctggggc tgacctgata taaaactgac acaagtctgg
     1021 tacaaactgg atttggatta gatttaattt gaattaaatt tggatctgtt ttgtagataa
     1081 ttttgatctg aaccatcgcg cattttgtga atggatgcag accattgcca gcgagcctta
     1141 ccgggcactg ccctcctcca gaagtgcata gggattaaag cgcatcacct cgatccccag
     1201 ccattcattg acatgcttca tggcctccat gacaggttgc agctcgttga tgaaaaacac
     1261 cctcgccgct ttctcaacgt caccgaacgc cgcgttgcca tcaggcatgg cccccatcag
     1321 ttgaggcggt acacgatggg ccgccaggat atcgtcgcgg gcactccctt tgatgttcag
     1381 gaattcatcc tttgcggtta tctgctggaa tggcagaatt tgcaccccat ccttgccgcc
     1441 acccggcgca tgaagaaaca ggtttttaaa cgccccgcga ccacgcgaat ccgtgagcgt
     1501 ttttttaata gcctcgacac tttccgcgtt cgcctgaggc gtaccaacat agataataca
     1561 cccggcatgc gagccattat cgtagtagag cttgcggaat gtatccgcag aatttgacag
     1621 gctgacggac agcagcccac caatatattc cggcatcccg tagatctcct gattaatatc
     1681 aggattaagc acgtggcaca cagccccttt ccggaaactg aactccgtac cgtcgtcgtt
     1741 gagataccag taggtatcca ggccacttcc cctgcgtgta tattttgcca gtgccgggcg
     1801 aagcaggagt ggttccccca gcctgttacg tcgtacttca aggtaggcat tgccaaacac
     1861 aaaccagtca agcgcaaacg cggaaaacgc ctgccgggaa agcagagaat gcggaataaa
     1921 gcacccgttc agggcgttgc gcttgaagta caaagccgac tgatgccagg acgcacgacg
     1981 ggatgcacgg gcgatagcgt tccagtccat gggggtatca aagtagcgcc cgttatctgc
     2041 acatgacatg ttatccagga gatccatgcg gctgacttca tagggtccat caaaggtgaa
     2101 tgcactgagt gccggtgttg atttcagtgc atcgggtagc gaatgctgcc cacgctttcc
     2161 ggtgtaattt ttgcgtttac tcattgtgtt cagaactcca ttgcaaagcc acggttatcc
     2221 tcccgctctg caccgattgg ctcattaatc acggcaagca ttgttgccca ggcgaggtcg
     2281 ccatggctga caccacggga gcggtcggtg tcataggtga tatagccgcc gggtgtctgt
     2341 atttttcgca ccgatgcgaa tgcctggatc aggtcacgtt cactgcggtc atactcccag
     2401 cgtccggcac gcaccacctg aagcattttc atgaccagtg cccgcttgga ggcgatattg
     2461 aagttgtacg gcacggccat cgggaaaaac tttttcacaa tctggaatac cccttcaccg
     2521 ttcccgccgg tcacatcaat accgatatgt ttcacgttat atttttgggt gtattcctca
     2581 atcagggccg cctgctgttc aaactccata ccctgtaccc tgaccgtctc cacggtacga
     2641 aaacgcccgc ctgcaaccat cggtggtaca catactgaca atgcaccaca gtctccctta
     2701 ccgctactgc cgttggcgtc gtacccgagc cagacttccc gcacgcccat cggacgcgca
     2761 gcaaaaggtt tccagtcagg ccattcgtcg tatccatcca caccacagcc gatcagcgca
     2821 ttcagactga aagccgcctc gccatcacgc atgaactcgc acatgtacag gttacgaaat
     2881 tcgctttcgg tgttttcatc ccggatatcg tcaatgctcg tatattccca cccgttcccg
     2941 actgcatcct caatggtgac aatctgccgc catgttttat ccgggaagag cacgccccct
     3001 ttcaggttcg aatgagacac atcgaattca atgcgttttg agcggggacg ctcactgttc
     3061 cagcgatccc ccgtccagaa ctgataagct tcatgcgttt cacccgatgg cgttgaaaag
     3121 taggtacggg taagcccttt cagggtcgcc attgcgccag ccacctttcg caactgcaca
     3181 aaattactga cccagaaaaa ttcatcaaat ttaaggtgtc cggtatacga ctgagcggtt
     3241 gctgccgatg tgccaaggaa gtgaagctct gcaccgttag acaaaacaat cttgtccccc
     3301 cctttcagct cgacatcaac ttccagcgcc gcctgctgaa taaagtgttt gaactgatac
     3361 gcctggcgac gggatgcgga caaaaatatc tggttgcgct gatacccggc agagacatca
     3421 tcacgcagcg catcaaggag cgcctcgtgc gcaaaatacc aggtagcccc aatctgacgc
     3481 gacttcagga tcatacgatt acggtggtgg cggttttcat accattcacg ctgatggcag
     3541 gccagtgtcc ggagggttct ttcacgcagg gcgatgattt gctcttccgt aaagtgattt
     3601 ttcagtttgc gcttgcgggg cttcttaccg gaaacagcct gttcaccctg cccctcgttc
     3661 agttttttca gctgacgcgt cagcaggtca atttccttaa aatcgccccc cgtcttatcg
     3721 gacttggcgg taagctggat cagtcgcgca tcgatggact gagtgacacg ctggatcggg
     3781 ggcgtggcat cccagtcatc ccgttttttc catgaataaa tcgtgttctg gttaatcccc
     3841 atcagttgag cgatatctgc cggagtgtac ccctgccagt aaagctgttt cgcccgcata
     3901 cggataaatg catcctggat cattgcgccc cctgccattt acacggcaag attaaaacct
     3961 ccttaacacc tcctgatacc tgccgccgtt gtgcctggcg ccatacaact tcaccacctt
     4021 gagacactcc cccgtacacc gccatcatga gtaaaacaac tctgctatgg agaatttcta
     4081 tgggaagtca aaaggcgccg gagcgaaaaa aatttcgcgt tgccgtatcc ggttcaacca
     4141 ttgacggacg cgagatttcg ggggaaatgc tgcgccgcgc tgcagaaaac tacgacccgt
     4201 ccgtttacgg ttcacgggtt aatatcgagc acgctctttc gctgtggccc tccagtgaac
     4261 tctgcgcgat gggcgatctg atcgcactgt caaccgatga aatcaccgaa ggaaaactgg
     4321 ccgggcgcac agcgttatat gccgagattg aacccaccga tcgcatgaaa aacatgctca
     4381 atgaagggaa gaaagtatat tcaagcattg aactgttacc ggaccttgac gccgttcagg
     4441 gaccttacat tgtaggcctg gcaatgactg atacaccggc aagcctggga actgagcgcc
     4501 tgaagtttgc cgcacagcag cgcgacagca tcatgcgctt caataaccgt acaagtgaac
     4561 cctccatgtt caccgcagcc atggaagccg aactgcttac caccgcagag cagcagacag
     4621 aggaaggccg ccagtggttc tcccgtgtca tgtctctcat cagcaaagcc agagggacag
     4681 acagcgagca gttcagccag atacgggaag tggtggaaag tatcgcctcc gatcatgccg
     4741 acctgctcga ccgcttcagc gctcttgaga agaaacacgc cagcggacag gaaacactga
     4801 aaaaactgca cgacgaactg gcagagctga agacgcaact gaaaaccctt gatgctgatc
     4861 cgcagcaacg ttttattgcc accggtggca ataaccagat tctggctgaa ttctgaaagg
     4921 agaagattaa cttatggccc gtttatccct ttcccgtgaa ggacgtgaca gtctggagcg
     4981 ctacatgctt cagcaggcgc agcttgctgg catcaccaca gaacggctga gcaaaacatt
     5041 cagcgttgat ccgtcaatcc agcagaagat ggagaacgcc atcaaggaaa gcgccgaact
     5101 gctgcagaaa attaatgtta tcggtgttga taaccaggaa ggtgaaaaag tcctgattga
     5161 caccagtggc cccattgcca gcacgaacgc aacgagcgac ggagtgaaac gccgtaatcc
     5221 tgcatcagtt gccgacctgg atgcgcgccg ttaccgttgc gaacaggtga actacgatac
     5281 cttcatcagt tacgcacaga ttgacgcgtg gagcacccag aagaactttc agcaactact
     5341 gagtgcacaa attgcccgtc agatcgctct tgaccgtatc atgattggct tcaacggcga
     5401 gtcgcatgca ctgatttcag atcgcgcagc taaccctctc ctgcaggacg tcaaccccgg
     5461 ctggctgaaa catatccgcg acaaggcaga cacccgcata gtgaagggga tgaccctgac
     5521 ccgtcgcgat gaagaaaaca aactggtgtc aaaaggtgac tatggtaatc ccgatgccat
     5581 ggtgcacgac acccgctcat ccgtgctgga tgaatggcac aaggacgcgc cggatctggt
     5641 tgtactgatg ggacgcgacc tgttcaatac gctgcgcctg ccgatgatta atgccatcag
     5701 caccaccaac ccgaacacag aactggttgc cggacagctg attgtggcat cccgtgccat
     5761 cggtggtctg ccgacgtatc tcgcaccgtt cttcccgaaa gacgccatgc tcatcacctc
     5821 actgagcaac ctgtcgatct acttccagaa aggttcactg cgccgttata tccgcgaaga
     5881 gccggaatac aaccggattg ccacttatca gtccatgaat gactgctacg tggtggaaga
     5941 ctacggcaaa tgtgcactga ttgaggggat ttcctttgcc ggtgaagatc cggatgagaa
     6001 gccggacagc gaggaatgac gacctgaagc aggcgcacag gcataaccgg ggcgcatacg
     6061 cgcccgtaaa tcaggagctg aaggatgctg acaccggcac aacgttatgt gcaacaggcg
     6121 gtggcccgta accgggtaac catgagtaca cgagaaacgc agcgggaaag aaccgcacat
     6181 gagcaggttc ttcaccagtt aaggctggcg cagcaacaac tgaagggtgt tcagtcaaac
     6241 atcgccagag cagaactcaa aagacacctg ctgcctgact ttgagggatg gatagaggga
     6301 accctggccg gaaacagcgg tcgccaggac gaggttatca ccacaatgat ggtatgggcg
     6361 atcgactgcc gtgactaccc gctggcgctg cgcatcgggg aatacgtgat ccgtcatggt
     6421 ctggccctcc cggataactt tggtcgcgat gccgccactg tactcacaga ggaaattgcc
     6481 gaaatcgtcc tgacacaggc cgccacaaac agcgatacag atttatcagg ctataccgtc
     6541 atgcttgata cgctacacgg gctggtacat gaccaggata tgccggatca ggttcgggcc
     6601 aaactgaaca aagcccgcgc tttttcccgc agggcatcca ctcagcctga tgacatccat
     6661 cagtcactga tgctgttgcg ggaagccatg cgcctgaatc cggccgcagg cgtgaaacgg
     6721 gacattgcca ctctggaaag agccgtcaaa aagctgtctt cgccccctcc ggatccggtc
     6781 aaaaaaagcg ccaaaaccac caggaaaacc ggcaacagtc aaaaaaccag tgcaaagaaa
     6841 aagccatcca ccagaaagcc caaaaacacg cactgattta aacgaactga cccacgtcag
     6901 caggcggcgc aaccggcgat ctgtatgttt tacacatgct ttttaccggt tgcccaccgc
     6961 ctgattttta cggagagagt tttgtatgag cctgatagca cggacaccgg cagagcacat
     7021 cagtgaagac attcctgata cagatgacgg agacagctgt gtgaccgctg acgcattctg
     7081 gcctgtcatt gtattacggg agctgagact ggctgttcgg cttccggggc gaacaaccac
     7141 ggcgcgcctg atgcatgccg ccacagaggc cgtcgctcac gttaccgccg aactggagat
     7201 gtggaaacag gaacgaatga aagaaggtta caccgccctg gcagatattc cggcaacgca
     7261 tcttaacagt gaaagtgtgc atgtccaccg ctaccgcagg gcggtgtatg cccttgcccg
     7321 tgcatttgtt ctcgaacgta cacgggatgt ggacaccaca gaaaaaggtg acaaaaaggc
     7381 tgactcactc gacccacagg ttgaagctct ctggcgggat gcccggtggg caatttcaga
     7441 tatacgcggc gtcactcgca tttatgcgga gcttgtctga tgaaaatcaa agcgctggaa
     7501 ggtgacaccg ttgatctgct ctgttttcgt cactacggca gtacacaggg cgtcacagag
     7561 caggtgcttg aagccaatcc gggattatgt gagcaggttt ttctggttgc aggccaggaa
     7621 gtggaactgc cggagcaaat ccgtgagaaa aaacaggaaa cggtattgct atggagctga
     7681 atatttttca gagaatttac gaccacatca ccttctcgat gtcactcatg gttacaggca
     7741 tcggtgtgat gacggtcagc gagaagatag cagttgccgg tcttctgctg ggcgcgctgt
     7801 ccggtcttcg cgcatggatc cacagggcgc ggctggaaaa agcgcaacga cgccgcaatg
     7861 aactgatagc acaaatcctt gcgcagtcag aatcgcgccc tctgaacgca tcagaaaaac
     7921 aggcgctgaa gcaactggag gatcaggacg atgccccgcc acattaagcg ctatgccgta
     7981 gccaccatcg tggcgctggg cgtgactctg gcaccgggcg ccctgcgaac ctctgaagaa
     8041 gcgcaggtaa aaatcgccac atgggaagac tgtcgggccc ggccttacta tgacacagcc
     8101 ggcattctta ccactggctg cggctcaaca ggaaacgtga aaaaccggtt atacagtgaa
     8161 aatgaggtgg ctgagcggtg ggttaacgac atgcagcgcg cagagaactg tatcaataaa
     8221 aacttccacg gtgccgccat gcctcagtct gtgtttgagg ctatgactga tgccgcctat
     8281 aacctgggct gtaccagtct gatgtggttt aaagacaggc gcgggcagcg tcaccggaca
     8341 acgatatgga agcatgcaca ggcccacaga tggatccagg catgtgcacg tctgaccgat
     8401 tttgtgaaca gtggcggcaa aaggatcccc ggactggtta accggcgcac agactttaag
     8461 gcatggtgcc tgaaggacac aggggctaca tcatgaaacc ggcaggcatc ctctttctga
     8521 tggtcttatg tatggtcgcg attgccatcg cattccggca gacatggcga ctggggcagg
     8581 aaagcgcacg taatgaggct ctccatgaaa ccatcagaag taacagcgcc acactggaat
     8641 ccatcatagc tatcagccgc aacactcacg cgctcattgc cggtctgaag gtggcagaac
     8701 aacatcgtaa tcaggaagga gaagtcagac gtgaacatat ccgcaatgcc atccgcagcg
     8761 atcggtgcgc tgacactccc gtgcctgctg ctgttgctga cagcctgcaa aaacgtaccg
     8821 cccgtctctc cccggagctg gtgcgaagca gtaccggaga gcctcaccac agtcacaatg
     8881 ccaccaccgc tgacggagcc agtgacatgg ggaaaaatcg cagtatggag tgacagcctg
     8941 ctggatgcac tggacagctg taacgcagat aaagcagcca tccgacaact gaacgctcaa
     9001 cagatggcaa caagagataa cgagtaaccg gagtacagta tgaaaaaata cgaactgatc
     9061 acagccatga cgtgtgatga gctggcaaaa gcactggatg aacgcctggc tgaaggctgg
     9121 caaccgcacg gagcccccgt ggcagcatca gaaccgggcg ttcccttcta tctgatgcag
     9181 gcgctgatta aatccacgga cactgcaacg caaggcagca acatcaacac acccacggca
     9241 gaaccggagt attactttgt gatccctgtt gccgggcagt caaacggcat ggcatacggc
     9301 gaaggtctgc cactgcccga aacctgtgac cgtccggacc cgcgaattaa gcaactggcg
     9361 cgtcgcagta cggtaactcc gggcggcgag tcctgcaggt ataacgacat catcccggca
     9421 gatcactgcc tgcatgatgt acaggacatg agcgggaaaa atcacccgaa agcagacaca
     9481 tcgaaaggac agtacggcac tgtaggtcag gggctgcaca tcgccaaaaa actgctgccg
     9541 ttcctgccat ctgatgcggg aattctgctt gtcccttgct gccgtggcgg ttccgcattc
     9601 acggcaggcg ctgacggtac gtacagtgac actactggcg cctcagggaa ttcagcccgc
     9661 tggggtgtgg ataagccgct gtataaggac ttaatcagcc ggacaaaagc ggcactggcg
     9721 aagaccccga aaaaccgcct gcttgccgtg gtgtggatgc agggcgagtt tgatattgat
     9781 gcgaagccga cggagcattc cgcgctgttt ctggcgatgg tggaaaaatt ccgcacagac
     9841 ctggctgaac aggcggaaca gtgtaccggt ggcagtgctg ctggcgttcc gtggatttgt
     9901 ggcgatacca cgtatttctg gaaacagaaa aacgaaccgg cataccaggc aatctatggc
     9961 ggctacaaaa ataaaacaga taaaaacatt catttcgtcc cattaatgac agatgaaaat
    10021 ggcgcgaatg tacccaccaa caatccggca gaagacccgg atattgaatc cattggttat
    10081 tacggctcaa cgtggcgtaa cagcgctgcc acctggacat ctgcggaccg ggaaagccac
    10141 ttcagttcgt gggcacgcag ggggatcatt tcagaccgcc ttgccacagc cattctgacc
    10201 catactgtcc gggctgctgc agtacagcct ggtgcatcat cttcagaacc tgatgtaccg
    10261 gaaaccgcag ccaccaccac gttactgtca tatatcgcca gtgaatcaga aggaaacctg
    10321 aaagcacagg gctggtcggc cagtggcggt aaagcagaaa tcatcagcga taccggagcc
    10381 acgggcggta aggcaatgaa actgaccaaa caaaccggca aaggatcctg ggttctggag
    10441 catgacgcag gcacgggtga cagtctgtta cagaaaggcg gtttaattcg ttgtcgcttt
    10501 aaagcgcagg gcgaactggc ggcaaaccgg tacgtcatgg cattgtactg gccggtatcc
    10561 tcactcccgc agaacgtcac cctggcgggc aatgcaggta acaacctgct ggcgtcgttc
    10621 tacatccaga cagacgcaaa agacatgaac gtgatgtatc acaatgcaaa agccgcgaag
    10681 gataaccaga aactgggaac atttggcgca ttcgataatg aatggcacac gctggcattc
    10741 cgcttcgcag ggaataacag ccttcaggta acaccggtta ttgatggtaa ggatgggaca
    10801 ccattcacac tgacacagtc acctgtcgga atatttactg cagataaact ccacgtaaca
    10861 gacgtcacta aaaacgccac ttaccctgtg ctgattgaca gcattgccgt ggaaatcaat
    10921 aacccggtaa ccgcgtaaaa aaaatcccgc cgctcattac agctggcggg aggtagtaat
    10981 acccaaccca ttaaaaattt gtaaggagta cccacaaagg agtaaatgtc agttgtctta
    11041 ctgttttacc cgataacgat ttatacggtc aatatcaaag acagggttat atggatcccg
    11101 tttacacaat actgctctgc ttttgcacca tcacctgcgt gctgttcctt gccagtggtg
    11161 gcggagaaat cctttatcaa tggtgcgcaa aaaaacttaa tgaatggctg aacaagtctg
    11221 aacgtgacac gccagaccag tgatctttac cggagaggtg acacatgctc aagatgaacg
    11281 cattgcgcga taccctggct gacacatgcc gctggtgtaa agccaatccg gataaattca
    11341 ctgtctgcgt ggaatccggc agcattgaaa cgaccggtga atcaccctcg tttctgtacc
    11401 gttacaacct ggtgatcttt gccatggact tcactcagga tatcgacgaa ctgttcctcc
    11461 ctctgctgca ctggctttac cacaaccagc cggatttact gctgaatcca gaaaaaaaca
    11521 gcagcatcaa attttcagca tccccgaata acgacgacag tgttgacgtg gttattgagc
    11581 tgccggtatg ggaacgcgtg agggtgacac gcacacctga caggcaagtc cgcgcagaac
    11641 atctgcagga gccgcggccc cgcatcccct cagcacgggg agcgtggagc aatgtatacg
    11701 aagacgtaac atggagtata ccccgtgatg aatgagcaca aacttgatgc tgtttttcgt
    11761 gacattctgg ccggtatgtc cgccgcaggt ctgtcccgca cggccagaca ggtggggcag
    11821 gcagtacgcc gcagccagca gcagagaatg cgtgcacaaa aagccccgga tgacaccccc
    11881 tgggcaccac ggaaacgtca gattcagcgg gtacaacagg gtattaagtt tctctggcaa
    11941 cctccggcag cgggcagcca gcccatggtc agggaactga aaaactggaa gcatggtaca
    12001 ggtcgccacg gcccacacat aaccggttat gacattaacc gcgggggact cagaacattc
    12061 taccgggctg acattgaaga atacctggaa atcagaacac gccgtgtcag ccagggaaac
    12121 accgtcaggg cacggatgtt tgaacgcctg cgacggtacg taaaaatact tcaggccgac
    12181 aatgagagca tccgggtggg ttttaacggt gatcaggccc gtattgcgcg tgtacaccat
    12241 ttcgggttac gtgacaacgt tgtccctggt gtaacaacac aatatccggc ccgtgaactg
    12301 ctggggttgt cagtaaaaga taaagcaatg atccgggaaa ccatcatcgg aagtctgaaa
    12361 ggagggatga agtgagcgcg gaaattatgc gcctgctgga aaatatgatc cgggtgggtg
    12421 tgatttcaga cgttgattta aaaaactggc gtgtaagagt gaccagcggc aggctgacaa
    12481 cggactggct gcgctggaac tgcacccgtg ccggtgtatt taaaatctgg cttcctcctt
    12541 ctcgcggtga acaggtttta ctgctctgcc ctggcggtaa ccctgaaatg gcaatggttg
    12601 tcgggagcct gtacagcaca gacaaccctg cccccggaac acagcaggat gaagtcatca
    12661 tcactgccag agatggtgca agactccgtt ataacgcaaa ggaaggtgcc cttgacgcaa
    12721 cagggataaa aacggccaga gtgacggcag aaacccgaat cactctggaa accccgctcg
    12781 ttgagtgcac acagcacctg aaaacgcgca catttgaact gacagatggc ggcacactga
    12841 aaggtgacgt aacccactca ggtgggtcac tgacatcaaa cggcgtggtc gcacacaccc
    12901 acacacactc aggcgtccag tccggtggga gccagacagg aggcccacag tgacagccac
    12961 ctacacgggt atgaaccctt accatccggg tgttgtgcac gatacagagc atctgcactg
    13021 ttctgtacgc gatattctgc tcacacctca gggaagccgc attatgcgca gggattacgg
    13081 cagcctggta cccgatcttc tggatgcccc caagaatgat gtaacccgcc ttcagtgcat
    13141 gagtgccgca gtgatcgccc tgacccgcca tgaaccacgc ctggcactga ataccatcga
    13201 cgttcgctgg ctggatgaag gtggggctga gcttttactg accgggataa tcaccgcaac
    13261 catgcagacg gtcagactga gcatacgcac aggagactaa ccgatgccaa ccgttgattt
    13321 gtcacagttg ccgaaaccgg caatcatcaa cacaccggat tttgaagtta ttctcaatga
    13381 gataaaaacg atgatgatcg gctgctttcc ggaagaacag caacctgctg taaccgctgc
    13441 tctcgcactg gaatctgaac cactgaccat tatcgccgag gtaatggctt tccgggagat
    13501 gctactgcgc cagcgtatca atgaaggtgt tgcagcctgc atgttaagcc atgcctccgg
    13561 tacagatctt gatcacctgg cagccaacct gaatacgcag cgcctggtga tcactccggc
    13621 aacagatact gccccgactg tcatggaaaa cgacaccgca ctgcgcttac gtgcgcagtc
    13681 agcctatgaa ggccttagcg tggcaggacc atcggcctct tatgaatact ttgcccgaag
    13741 tgccagtggt caggttgctg atgccagggc ggtcagccca tcccctgcag tagtggtggt
    13801 gtcaattctc tcaacagaag gagacggcac tgcatcgcag gaactgattc agaccgtcag
    13861 taacgccctc aacgatgagc gaatcaggcc tgtcgcagac cggctgaccg tccagagtgc
    13921 aaaaatcgta aaataccgga tcaatgcccg gctctttctg tatccggggc cggagtccga
    13981 acctgtaatt ctggctgcca gacagtccct tgacgaatgg ctgaccgaac agggccacat
    14041 aggccgtgat gtggcccgct ctgcgatcat gtcagcattg catgtacagg gcgtccagcg
    14101 ggtggagctg gaggaaccag caagcaatat cgcggttgac gactcgcagt tcgcacactg
    14161 cgaatccatt atcgttgaga tgggaggtac caatgagtga ttccctgctt cctccttcag
    14221 ccagtgactt tctgaaatat accggatatg ccactgaccg aatcacccgt ataccggttg
    14281 atttgcgtac tctctgggat ccggaagcct gtccggtgga gcttctcccc tatctggcct
    14341 gggcgctgtc tgtcgagcgc tgggataaaa actggccgga ggaaacaaag cgaaaagtta
    14401 tcagggaatc ctggctcatc caccgtcaca aaggtacctt cagctcactc aggcgcatcg
    14461 ttgaacctct gggttacatc atcagcatga gcgaatggtg ggaaaccggc gatccacccg
    14521 gcacttttcg cctgaatgtg ggcgtgtttg aaggggggct gactgaagag atgtattttg
    14581 aaatagagcg actaatcgcc gatgcccgcc ccgtcagccg tcatctggta ggcctgaata
    14641 tcgtgcagga catttccggc aaggtataca caggcggcat ggtgtatgac ggcgatatta
    14701 ttaccgttta cccgatacag gagagcgacg catgagtgat acggtaaagt ttaaaaccat
    14761 tatcacgact gccggtgctg aaaagctggc agcctcagcc tctgacggcg gcaaaaaagt
    14821 cacgccagcc gttatggctg taggtgatgg cggcggaaca ctgccagatc cggatgcgaa
    14881 acaaacagcg ctcatcaacg aagtctggcg acacgatctg aacagggtat ttattgacaa
    14941 taccagcaaa aataccgtag tggcagaact catcattccg gcagaaacgg gcggtttctg
    15001 gatgcgtgag ctgggacttt atgacgatga aggaacactc atcgccgttg ccaatatggc
    15061 agaaagctac aaacccagaa tagccgaagg cacaggacgt tcacaaattt gccgcatggt
    15121 tatcgcggtc agtaacattg aaataatcag tctgactgtt gattcaacgg tcgttaccgc
    15181 aacgaaagaa tacgttgata atctgcttga agagcatgaa aaatcgcgca atcacccgcg
    15241 cgccagtcag gccgcaccgg gattagtaca actgagcagt cagacagatc ttgactcaga
    15301 aataatggct gccacgccaa aagcgataaa aatcgtcatg gaaaacgcca atggtcggct
    15361 tgcgaaaaac cggaatggcg gagacatccc caacaaggag ctgttcctga tcaacgtggg
    15421 cgcaccacgc gtttatgcaa aagacgggta cgtgggtgaa gcagggcaac gctggacaac
    15481 agaacaattc gtggaatggc tgaatgaacg tggcgccttc agaccaggac tctggatatt
    15541 caaagccaat atttcacttt cccggcacag cgtgctcact gatacgggct atggtgatat
    15601 ctgccttgga ggctgcctta tcgaagtatc aggcgacatc aacgggggga tcatcaggat
    15661 cacaacaggt ctcgaaagcc caaaccctgc cgacatgtac ctccgcacac agttcaccgg
    15721 ggttgtttta aataaaggga aaagcaccat ctggtaccgc acttacacgg acaaaaacaa
    15781 acccacacca caggacatcg gcgcactgcc tgcaagtgag ttgttacccg caggcgttcc
    15841 cctgccgtgg ccgtcagata ccccgcctgg cggttacgcc atcatggtgg gacagatatt
    15901 cgataaagca cgatatcccc tgctggcaaa agcctatccg gatggtcgta tcccggatat
    15961 gcgtggctgg acaatcaagg gaaaacctgc cagcggtcgt gcggtattgt cacaggaaca
    16021 ggacggcatc aaatcgcaca cccacggagg gagcgtctca aatactgacc ttggcacaaa
    16081 aacgacctca tccttcaatt acggcacaaa aacaacaaac agcgcggggc cgttcagcgc
    16141 aacaatatca ttaagaaccg ccaccgcgtc accggaaggc agcggtgact ggaccgcata
    16201 cgggccgggc tccggggcga acctgatcgc cagagtttcg gaacacacac attcggtaag
    16261 cattggcgaa cacatccaca cgctcgcact gggtcaacat ggtcacggcc tgaccattaa
    16321 ccccaccggt aatgcggaaa acaccgtcaa aaacatcgca tttaactaca tcgtgaggct
    16381 tgcataatga cctttaaaat gagcgaacac ccacagacaa ttaaaatcta caacctgtca
    16441 gccggaacaa atgaatttat cggcgaaggc gatgcctgga tccctccgca taccggactg
    16501 cctgcgcact caacggacat tgccccgcct gacattcctg aaggacacgt ggctatcttt
    16561 gatgaggacg aaaacacctg gcacctcatg gaagatcacc ggggtaaaac ggtttaccac
    16621 gaagagaccg gagaaaagct ggttataacc gggccgggtc ccctgccatc atccgtgacg
    16681 accgagccac ctggtacttc agcagatgta taccgtgacg ggcaatgggt gacagattta
    16741 tcagcagcgc gcgggatcaa aaacagcgaa attaacgact ggcgtaacag aatggaaaac
    16801 gctggttata ttatggaata caatggccgc aaatgggatt acggcaaagc cacgcaggat
    16861 cgccttgagc catctgtcac agccgccaga gccggaaagt taccggcaga gttcttctgg
    16921 acagatgcgg aaaataacga tgtaccaatg agcgcagacg aactgatagc gctcagcgag
    16981 gcagtcggag aggcaatgtt taaaaaagga cttgaaattc acatccggca gcgggaaatg
    17041 aaaaaagaac tggccggaat ggataacgcg ggggaaataa tgaaattcac tccgggctgg
    17101 aaaacaacat gacagaatga ggcggcagac ttcatgatgc tctgccgccg gatatcacag
    17161 aatgccagaa gccattgaag aaacccgttc tcccgccgca ctggcagcgt ctttcaggtt
    17221 attcagcaaa tcccccacag atgatgcctg aagacgctcc cgcaaatcct cgtcacaacg
    17281 gcggaaactg acagaaaacg taattttttt cgcccgtcca tagcgatcaa actcttcacg
    17341 ggtcgtctga atatccgtaa tcacatacat accgtaaatc tgtccggctc cgtcaatcaa
    17401 cggccagggg cgccccgtat atgcctgagt gttcagcacc gtaagggaaa cctccccgcc
    17461 cgtgatttcg ggatacagca caccggaaag cgtgatttta tcttctccgg ccccgatata
    17521 ctgccagcca gcagaacggt taatacgctc attgctgaca tggcgccagc cccggctctg
    17581 ctgtaactgc tgatgtggca gtgtactgag ctgaaaaaca aacataccgt aaatcatcat
    17641 catctctcat accctccatc atccattaat ccacatccct gaaactacgc agcccgttac
    17701 gggactgacc ggacaattcc gcttttaccg cttcgccaat catccgggca agagttcgcg
    17761 gatcctgggt aacgacatta tgcagatgaa catggatctc cccgctgaac cccacagaag
    17821 acagtccgcg ttctgagcca gatgaaggca cagtgagcgg tggtggactg gcaaccttaa
    17881 tgttctggtg ctcttcaata cgggctcctg aaacaccagt actgcccgcc agtcggggtt
    17941 ctgtccattg tccgcgaaca accagcgccg gaggaagatt acgaaaaata atatcaccag
    18001 gtcccgtgcg ctttttcgtt tccctcacga tgtcccgtgt attaccttcg atacctttca
    18061 gcctgtcaag aacgccacca ttcccgctgt ttgcggaggg ctgcaacacg gctggcattt
    18121 caggtaagcg tagggcaccg acactctgtg ttcctcctgc tcctttcggc gaccagttcc
    18181 acgccttctg aaccatcttt ttctgctcag ggtcccattc ccagaccaca ggtgttttaa
    18241 cagccgaatc cagtttctgt ttcacgcgtg ctgcctctcc gggaagcacc ccaagcttct
    18301 ccagcaccca ggcaaggcca tctatcagcg ccttcatcgg tgaaaacaca agcgtcagag
    18361 cactgaccag agaactgcca aaacgtgctc cggcaactgt acacttatcc agcgtgtcac
    18421 gcgttgctgc gacgggactc aggagcgact taaaccacag gaataccctg gatacagcac
    18481 cggcaaccag atccagcaac ggtgcaaaac gggacaccgt ggcaagcaaa ggtgcaagcg
    18541 cccctctcgc accatcgata aaccccagaa caaacgcctt gacctgatcc cagtatttat
    18601 agatcagaag cccggcaccc agcagcgccg ccacaaccag accgacaggt cctgtaagca
    18661 ttcccaccgc agtacccagg gaagaaatta cgccccccag accaccaaag agaccagaaa
    18721 caacggaaat acccgcaggc agagctctta tcagactcag accaagacgc agttttacca
    18781 gggggattaa caccataccg acggtaacaa ggagtccgcc gatggcagca gacagcccgg
    18841 caacaacacc ggcgaccata agcagtcccc tgaccagttc aggatttttc tgtacccact
    18901 cccgcattct ggctgtccac tgcgtcgccg tctgtaccag cccgcgcaat gtttctgtta
    18961 ccgtactgac agcatcagcc cgtaacccgg caagcgaacc cgtcagcttc agaacgtcac
    19021 cggagagatt atcacgcagc gtattcccca ttcgctcagc tgcgccttct gtgtttccaa
    19081 gtacgacaga agtacgggca agtgccccca gaaatgctgg gatctgatca actgccaggt
    19141 cctcaacagg cgtaccaaac aacgcaatag ccgcattagc ccgttcagcc ggatcgcgaa
    19201 tgcgcagtaa tccggctgct gttttctgca tggcatcacg ggcggcgacc ccgccactgg
    19261 caatagccga agacatctgc gccgcattaa gccctatgct ttcataagcc tcgacgcttg
    19321 acttcgacat atcagaacca cgaatactga actccttcac agcatccccg gttttatcca
    19381 gtgcaaattt cccctgacca gccatatcag ccagcagact catcgcctca gcaccgctga
    19441 aacccagatt tctgaaatgc gttgaatact catgaataat ttcaggaagt tcaccacgca
    19501 tctccggccc catgcgttgc atggcagaga ccatcagatc gagagcctca tcactgcttc
    19561 gcgccagccc gtttttcatc atgatggcag cagcctgtat cccctgagga acatccacgc
    19621 ccatcactgt ttgcatgtcc atcatgcgac gggtgatacg ggagatctct gtatcccccg
    19681 taactccaag cgcccccagc gtactacgaa cagctcccac tgcttcagcc gcccgggcag
    19741 cgtcaccagc aataccggca tgatgcagtt cacgaacaag acgtgtatac ccggatgcct
    19801 cagcacgccc ctctccggtc tgggcagcaa tcactgctcc atagcgctct tcattcatca
    19861 ccggagcaac catgcggctg cccgcataca tcccgccaag accggcaagc atcattgctg
    19921 taccagcgcc acgcagtttt tcagataccg ccatcgcacg ggtatactca tcccttgccc
    19981 ggctgacccg cgcaagcgcc agacgctgcc gctcgagctc ttcgttatac tgttgagttc
    20041 gcctgattgc cgcctcaatg gccctgtccc caccagacaa cgtgacacca tgtcgcctga
    20101 gcgcttgccc ggcttcccgt agtctgtttc tctcctgcgt tctgacttcg ttcagccgtt
    20161 caagtcgtgc cgtcagcttg ttaatcaatt cctgctgcgc ttcagtgagc ggacgaccgc
    20221 tctgctgaag acgctgaagc ccgttcagtt cccgctgggc gcgcttaatt ttgtctgtta
    20281 cttcggtgag atgtttgcgg gcagatgaaa aggagcggga ctgtctttca agatcccgga
    20341 gattattttg cgtattgcgg agagaggcgg ccagcgcttc tgcactttca cgggcactag
    20401 tggctggccg ggtcaggtta tcggctgcac tgaatgcaac acgaatatca agtgtattca
    20461 tttttctgga gatccgtttc tgtcagacgc ccgcttacgc caggcgatga catcctccgg
    20521 ccccatcata aacaggtcag cgggcgacca gttaaaaaca actgcaatat cagcgacaac
    20581 atcctcgata tgttcaaact gcaggatggt gatcagccgt ccgtctcctc cccgttcggt
    20641 tttgtcgaca caccaccgac ataaaaattt acggcagcat tcgcaagcgc agaaaaatcc
    20701 cttgcatcca ttgccttcag ttcactctct ttcaggcggg gctctgtcac acgggagaga
    20761 tatgccagca gcgcatcaaa tttcatgcca tacaaatcca ccagagacag cccccgcagc
    20821 gaaccagcct ggcgggcaag tggcccaatc ttcactgatt taatggtttc agtatcacgc
    20881 acaaccggag taatcaggct gataacatcc ggagacgcat tacttccgat atctttgtca
    20941 gtgaccagtt ctgccgtttt cgtcagtttt gcattttcat tcacgtttta ttctcctggc
    21001 ggtatggcta ccgccgtctg ttctttcaac ccatccccag cgcggaacgg atgcggtcgg
    21061 gaacaatatt ttccccgtcc tgtcggtaga tgaagttcag caaatcgaac tccagcagtt
    21121 cacgatcatc tgaactcagt ttgtaatacg tgtttttcac attgtaggta tgctgggtat
    21181 cttctccctg ttttgcttcc cccatatcga tttcactgat gcgtccgcgc atttcaacct
    21241 caatcaactg actgtcgcca tcggtgtaat actctccggc aaaacgaaga cggacctcat
    21301 caatacgtcc ggcatactta cgcaccaggt cagcagctgc accacccata acaaaactcg
    21361 catccagtgc gctgtcatcc agtccgagat caacagccac acttccaagc atacctcccc
    21421 cacggtagct ctccgttttg cgcgtcagtt tgggtaacgt gacagacacc acctgaccaa
    21481 tttcgttttt accgtctatg aaaagcgtca taaaacgcag cttatgcgga accgccatta
    21541 cgcacctccc agagaagcaa atgcaggacc aaagaattca tccgtaaagg tctgatgcag
    21601 agtcagatct tcaagtggtg gcacaggcgt gtatttataa cgaatacgca cccttccctg
    21661 acgcaaatcc gttgtgctgt tatcaatcac gtcataccag cattccgccc caatcagttt
    21721 tccggcagtt accagttcat taagttttgc ccgaatggcg ctgatggcgt ctttcacatt
    21781 tgcaggcgtg agcggatcat caacaacctc aaactgcgct tcggcgatgg tatccgccag
    21841 agtctgtgcg gttcgtgtat agacttcaaa aatgtaggcg ctggtatccg tggtacggtt
    21901 tccccagaag cgaaaaccat tacgtctgat aattgtggtg atttctttgt tattgaggtt
    21961 attggcatca ctgtcttctg actggagaga ccagtagacc ggatgagaaa cacccagcac
    22021 attttttacc ggttcattag acagcgactt gtgccagccc tgttcatgat caagacgggc
    22081 ccgaagaccg caggcataag cagcagacgg gaagctttca ttctttcctg actgtgggtt
    22141 ataggcgata aaatccggcc agatcagcat cagttcccga taagcaagat cctgacgcca
    22201 ggtcatggct tctgtggcag tattgcagcc gggacaccct gcataaacaa acgcccgcaa
    22261 tttaccggca atcgtgcaca acgcagtggt aacggctttt gtgtcaagcc caggcgccgc
    22321 cagaatacgc ggacgatacc cgatagcctc atcctgctct gctaccagaa gcgcctgcat
    22381 accggtatag cgaccatccg cttcaaccgc accaataaca agctggtcct gagtcttctg
    22441 tttgtcacct ttcttttctg ccgccacacg aacgacaatc acctttgtgc tgacctgatc
    22501 ggatatcgcc ttcagggctt tatacagcgt acctgccttg cctgccttgc ccagcacatc
    22561 attcacacgg gtcagcagaa caggcttatc cagaggaaac gtgccggtat cggcatcatc
    22621 tgccacagca acaataccga tcacacttga gtcgatatcg ttaattgccg tgacgagatc
    22681 ggtgctttcc cggacacgcg ccccgtgaaa acgtgtttcg ctcatacatt tcaccatcat
    22741 cttcagaatg aattcacgat gatgattacg cagatacacg gggagttcac gatatacagg
    22801 ttgtgaatca tcacatacaa cctgccgggg ttccggccag acggcggtta tgagattttc
    22861 gccacagata acgggagaac acaacaatgg ctgatcggat cattcgcatc ataaaaaact
    22921 atgatgacag gacgagtacc ctggtaaagc ttcctgattt cagcatcacg ctgaacaaca
    22981 acgaactgga agcacaggaa ttaaaaaaca gggtaatgtc gctgtctgta actgacaaca
    23041 ggggatttga agctgatcag atatccctga cactggatga tacagacgga caattaatcc
    23101 tgccggaacg gggagccgta ctttccatcg ccattggctg gaaaggcgaa agcctgacag
    23161 ataaagggac atatattgtg gatgaagttt cgcatgacgg gccaccggac aagcttatca
    23221 tcggagccag tagtgctgac ttccgggaag aattcaatgt caaacgggaa gtatcctggc
    23281 atgacgtaac gcttgagcgt gtggtttccg ccattgcaca ccgatacggt ctgaaagcac
    23341 aaatcagcga aatgctgatg catatcgaaa tcgaccatgc agaccagaca gaagagagtg
    23401 acatgtcatt ccttacccgc atggccgata tgcttggcgc catcgcaacg gtcaaaaacg
    23461 gcaacctgct gttcatcata cctggtggcg gcgtgacagc cagtggcaag ccactcccct
    23521 ctttcacctt aacccgcagc gacggtgatc aacatcgctt ccgtatcgct gaccggcagg
    23581 catacacagg ggtacgggca tactggctgg atctgaattt cgggaagaaa aagcaggtca
    23641 gtgtaaaaaa acgcagtctg tcacaggaaa aaagccacag ccgtgaaggt gattacatgg
    23701 aaggcgcaga aggcaatgtg tttgtgttgc gaagaacctt tccgaatgaa gaaagcgcaa
    23761 aacgtgcagc agctgcaaag tggcaacaat tacagaaagg cgcggctgaa tttgccatca
    23821 cactggctca cggacgcgca gatctctacc ctgaaatgca cggcaccgta tcaggattca
    23881 aaaccacgat cgaccggcag gactggatca ttgccagggt ggaacacact atcgacagca
    23941 acggtttcac cacacaactg gagcttgagg ctaaaatacc tgactggata gcagaatcag
    24001 aacagagtgg ataaaataaa ccacgagttc aaccccgtaa tgactggggg tatcgtcatg
    24061 ttttattgtc cacactgcgg cgcatcggcc cgtatcagaa ccagcaaggc agccaatgat
    24121 accggcacca tccggtataa atactaccag tgcaacaaca tcgaatgtgg tgcgacattc
    24181 tccacaatgg agagcgtaca caaaatgata tccgtaccca ccccctccgg cgaacgggat
    24241 gaacagatcc cgcacgagca tttcccggca acacacagag gtcgccttca gctaatcctc
    24301 gagctctgac agttgcgacc acaaaaggaa atcagtacat tacacgcggg tgcctttcgg
    24361 ctgacggctg gagggtgaac ctgaaggccg gatgtggaaa ggccccgagt caacttaaac
    24421 gttaacccga ggccctgacc atcctaccta gcaagtggaa ggttagcgcc tccctgtaaa
    24481 aggagtcaag cgctatgccg caaaaaacga tcatagtgag cattttatgc ataactattt
    24541 tgatgattgt ctggatgata cacaactcac cctgtgagtt caggcttaac atcatgtggt
    24601 cacaatttgc ggcgttcttg cagtgtaaac agtaagaaac cgcagcgggg gtgaaagccc
    24661 ccgctatccg gttgccgggt tcagtcgata tggccccctt ttctccattg gattacagac
    24721 gtatttgccc gcaatatgcg ggcttttttt gccgccccct caacagcttc aacacagagc
    24781 attgcgcaca caaagggaaa tcagtagatt acacgcgggt gcctttcggc tgacggctgg
    24841 aagggatacc tgaaggtcgg atgtggaaaa gccccggaaa aacacttttg tttaaccgag
    24901 gccctaaccg cattaccttg acaagtgaaa ggttagcgcc tctccactaa ggaggcaagc
    24961 gccatgtcgc aaaaatcgct gacagccatc acgttctgcg tgacggcaat cctcatcatc
    25021 tggatgctgc acggttcact gtgcgaaata cggatgagct tatggggagc ggagtttgcg
    25081 gcgttcttac agtgtaagca gtaggaaacc gcgacgggag agcaatcccc cgtcaatcgg
    25141 ttgttaaggt taaggctgaa aaggcaccct atctgttact tgaaatgaaa agcacagccc
    25201 gcagcgtaaa aactgcgggc tttgtttttc agtactatcg aaaacagccc acacctacaa
    25261 acagtgaata gtacagagag caataagaaa ccagcaataa caggaaagac gattgccact
    25321 gagtcaactt tactcccgcg cctacatgtc gccataccat cgccacttta tcgccacaag
    25381 ccaaaacaca atcacaaaaa aaccaccct
//
LOCUS       DAZGCQ010000071        25383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000071 DAZGCQ010000000
VERSION     DAZGCQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.071"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..171)
                     /locus_tag="ACHK07_003213"
     CDS             complement(<1..171)
                     /locus_tag="ACHK07_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8103509.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPV"
     gene            388..723
                     /gene="iraM"
                     /locus_tag="ACHK07_003214"
     CDS             388..723
                     /gene="iraM"
                     /locus_tag="ACHK07_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO8103510.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVPPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECFNNHCILQIC
                     PYGLKQQCP"
     gene            complement(1025..1189)
                     /locus_tag="ACHK07_003215"
     CDS             complement(1025..1189)
                     /locus_tag="ACHK07_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO8103511.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(1186..1617)
                     /locus_tag="ACHK07_003216"
     CDS             complement(1186..1617)
                     /locus_tag="ACHK07_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8103512.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1807..2016)
                     /locus_tag="ACHK07_003217"
     CDS             complement(1807..2016)
                     /locus_tag="ACHK07_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103513.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(2069..2293)
                     /locus_tag="ACHK07_003218"
     CDS             complement(2069..2293)
                     /locus_tag="ACHK07_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103514.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(2383..2459)
                     /locus_tag="ACHK07_003219"
     tRNA            complement(2383..2459)
                     /locus_tag="ACHK07_003219"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2423..2425),aa:Arg,seq:tct)
     gene            complement(2473..2549)
                     /locus_tag="ACHK07_003220"
     tRNA            complement(2473..2549)
                     /locus_tag="ACHK07_003220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2513..2515),aa:Arg,seq:tcg)
     gene            complement(2559..2634)
                     /locus_tag="ACHK07_003221"
     tRNA            complement(2559..2634)
                     /locus_tag="ACHK07_003221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2599..2601),aa:Ile,seq:cat)
     gene            complement(2675..3732)
                     /locus_tag="ACHK07_003222"
                     /pseudo
     CDS             complement(2675..3732)
                     /locus_tag="ACHK07_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3883..4080)
                     /locus_tag="ACHK07_003223"
     CDS             complement(3883..4080)
                     /locus_tag="ACHK07_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8103515.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4296..4676)
                     /locus_tag="ACHK07_003224"
     CDS             complement(4296..4676)
                     /locus_tag="ACHK07_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8103516.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4694..5683)
                     /locus_tag="ACHK07_003225"
     CDS             complement(4694..5683)
                     /locus_tag="ACHK07_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8103517.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRLAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(5735..5993)
                     /locus_tag="ACHK07_003226"
                     /pseudo
     CDS             complement(5735..5993)
                     /locus_tag="ACHK07_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5990..7390)
                     /locus_tag="ACHK07_003227"
     CDS             complement(5990..7390)
                     /locus_tag="ACHK07_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO8103518.1"
                     /translation="MTNPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWAKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMTSLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRHIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTQGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKAERLF"
     gene            complement(7387..8277)
                     /locus_tag="ACHK07_003228"
     CDS             complement(7387..8277)
                     /locus_tag="ACHK07_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8103519.1"
                     /translation="MTNFLNKQHMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDRVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(8297..9208)
                     /locus_tag="ACHK07_003229"
     CDS             complement(8297..9208)
                     /locus_tag="ACHK07_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8103520.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEESWQQPA
                     APGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(9205..9429)
                     /locus_tag="ACHK07_003230"
     CDS             complement(9205..9429)
                     /locus_tag="ACHK07_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103521.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNIESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(9426..10289)
                     /locus_tag="ACHK07_003231"
     CDS             complement(9426..10289)
                     /locus_tag="ACHK07_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8103522.1"
                     /translation="MTRHQHIDSASPGRYTVPAPYKTGAGIGVLKLITEHNRAHAVFS
                     CHEHYYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKSLSGADAS
                     DMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            complement(10286..10918)
                     /locus_tag="ACHK07_003232"
     CDS             complement(10286..10918)
                     /locus_tag="ACHK07_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO8103523.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELLQATNDAPASFLLSASLHARI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEAALMATIPAHPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(10947..11123)
                     /locus_tag="ACHK07_003233"
     CDS             complement(10947..11123)
                     /locus_tag="ACHK07_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8103524.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFIPLW
                     LFRRDFVECEAPPT"
     gene            complement(11143..11850)
                     /locus_tag="ACHK07_003234"
     CDS             complement(11143..11850)
                     /locus_tag="ACHK07_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8103525.1"
                     /translation="MNTTIFTDKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRLERVEEKVSHVTETVEQIKRGAIRDGYAGYRQLAAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMTSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(11847..12110)
                     /locus_tag="ACHK07_003235"
     CDS             complement(11847..12110)
                     /locus_tag="ACHK07_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8103526.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            12234..12938
                     /locus_tag="ACHK07_003236"
     CDS             12234..12938
                     /locus_tag="ACHK07_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO8103527.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            13198..13785
                     /locus_tag="ACHK07_003237"
     CDS             13198..13785
                     /locus_tag="ACHK07_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8103528.1"
                     /translation="MSENGAESERVYNAIRCLMRSLQKTEETASIFAEKLCEAKAMVS
                     HGHFFGKIRAQREACGLTTTELAKLLDLDEEIILQWESGEFEPTISMLIPLANVIGCD
                     PLSLLDEKDSASVIRVNAPEVYVESIGARIKSARKKLGLTEADLARMINTYSDPINDW
                     ECGICEVPADQIVPLASALNCDLMWLLTGKSEAKE"
     gene            13791..13991
                     /locus_tag="ACHK07_003238"
     CDS             13791..13991
                     /locus_tag="ACHK07_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103529.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            14190..14372
                     /locus_tag="ACHK07_003239"
     CDS             14190..14372
                     /locus_tag="ACHK07_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103530.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIA
                     EKAGYDVIAPADEDEE"
     gene            14378..14950
                     /locus_tag="ACHK07_003240"
     CDS             14378..14950
                     /locus_tag="ACHK07_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103531.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDLNTHGMPEGQ"
     gene            15135..15323
                     /locus_tag="ACHK07_003241"
     CDS             15135..15323
                     /locus_tag="ACHK07_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103532.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            15320..16147
                     /locus_tag="ACHK07_003242"
     CDS             15320..16147
                     /locus_tag="ACHK07_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO8103533.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSDANTPFIQVK"
     gene            16188..16559
                     /locus_tag="ACHK07_003243"
     CDS             16188..16559
                     /locus_tag="ACHK07_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO8103534.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            16591..16833
                     /locus_tag="ACHK07_003244"
     CDS             16591..16833
                     /locus_tag="ACHK07_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8103535.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            16837..16971
                     /locus_tag="ACHK07_003245"
     CDS             16837..16971
                     /locus_tag="ACHK07_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103536.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            16990..17244
                     /gene="xisR"
                     /locus_tag="ACHK07_003246"
     CDS             16990..17244
                     /gene="xisR"
                     /locus_tag="ACHK07_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO8103537.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            17278..18564
                     /locus_tag="ACHK07_003247"
     CDS             17278..18564
                     /locus_tag="ACHK07_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8103538.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            18566..19345
                     /locus_tag="ACHK07_003248"
     CDS             18566..19345
                     /locus_tag="ACHK07_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO8103539.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            19398..19793
                     /gene="yecN"
                     /locus_tag="ACHK07_003249"
     CDS             19398..19793
                     /gene="yecN"
                     /locus_tag="ACHK07_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO8103540.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            19834..20577
                     /gene="cmoA"
                     /locus_tag="ACHK07_003250"
     CDS             19834..20577
                     /gene="cmoA"
                     /locus_tag="ACHK07_003250"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO8103541.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            20574..21545
                     /gene="cmoB"
                     /locus_tag="ACHK07_003251"
     CDS             20574..21545
                     /gene="cmoB"
                     /locus_tag="ACHK07_003251"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO8103542.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(21710..24138)
                     /gene="torZ"
                     /locus_tag="ACHK07_003252"
                     /pseudo
     CDS             complement(21710..24138)
                     /gene="torZ"
                     /locus_tag="ACHK07_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(24163..25263)
                     /gene="torY"
                     /locus_tag="ACHK07_003253"
     CDS             complement(24163..25263)
                     /gene="torY"
                     /locus_tag="ACHK07_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO8103543.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAVGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         6520 a         6568 c         5884 g         6411 t
ORIGIN      
        1 cacaggtgtg gtgacatcac cttccgccgc aatggcctgc atcagggtat aaggcgtgat
       61 ggcgacaggg ctgccaaatg gctgccaccc ctccttcagt ttttgagtca gtcgctccgc
      121 aaggtctgac ggcgacgccg ccctgaccac atcgtagtgt ttaaatgcca tggttctttc
      181 caccatatta aaaacaattc tttaaaatac ctgacatgca atacagaaaa aaaacacaaa
      241 accatacctt aaatgaaaac ctcatcataa agcaggtatg catggataaa ccacatcaga
      301 aaaataattc tgctctatgg tttacattca aaattatcat ttatactttt cagaacatca
      361 ccagcacagc ataaataagg aggctaaatg aagtggattg tgattgatac agttatccag
      421 ccatcatgcg gaatatcttt ttcagccata tggagtaaaa taaaattaat aatctggtat
      481 caatcggatg ttttcttacc tcctgaaagt atatttacgt tgactcacac aggtatcgta
      541 cttaataaca aagtgccacc tgtaaccatt tacaacgttg taccattcaa taaaagattc
      601 tgggatttaa tcaaaaacag ccaggaatgt cctgcaaatt cagataacgt attgaaagaa
      661 tgctttaata atcattgcat tctgcaaata tgcccttacg gactaaaaca acagtgtcca
      721 taattagttt actcatatct tacaaaattc atataaaagc ccctccggag aggggctaaa
      781 gccgcgtatc tgtatcatca tgcgcatgat gccgggtgcc tcccggtgag ttcagcccgg
      841 tgccaccaaa cccgcgcgtt cactttcctg ggtatcaaag ataacaccta atgcaccagt
      901 cgcccctccg ctcaggggga ttcaccatgc cagtttcttt taacaaactc cccgcaaaac
      961 agacaactgt caaccgtctg aattgtgaga catttaaaat tttcggggca tgactgatac
     1021 ccggctaact acctggcatg tcctttttca ccaaaggaaa aagcaccacc acaataccga
     1081 ccaccagcac tccatccgcc agcaccgaca tgatcctgct ggtgaaatcc accatcacca
     1141 ccagaaacag caggagtgcc acagcggcca gacgcagttt taccgtcaca ggtggttctc
     1201 cagtcgcagg ccaagaacac cagcaatctc ttccagaacc ttacgctctt ccggctcaat
     1261 ttcaccatct gcttcagcaa tagccaccgc cacatccagc acatcttccg cttcacgcgt
     1321 atcgtgtttc acatcttcaa tttcacgcaa cgccgctcga cgaccaattt taaagttggt
     1381 gtcaagctga cctgtgatcg tggcactgat ggcattaatt tctgacgtaa acgcggacaa
     1441 cgcaggctgg ttacgtaata cctgttcgat cttcgctttc tctgaagcct cacattcacc
     1501 atctgcatag gctaccaggt aggcagcatt aataaccgcc tgtgccagat cgcgtttctc
     1561 aaacttttta atttccgttg ctgctctgcg ggttttcttt ttgaaaatac caaacatcgt
     1621 gacgttcctt tgggtgggtg agccaacgcc cgggagcgat ctgcccacag agaaagtcac
     1681 actgaccact ccgtaagctc acccccgaaa ggctctgtgg ttgatatgcg ccgggcgtgg
     1741 cgcggataca aaaaaggccg ccaatagcga cctcagttac gggattattc tggggttaaa
     1801 cgactgttac tccccccaga caaaatcatc acttcctgtt cgatgcgagc catagtgaac
     1861 ctcgtactta tctcccatct ttcttgcttc cgtttctgcg tcttcctctg tcgcaaaaac
     1921 cccaacaaga tgccagggcg agcttcttac cacagcccaa cctttaaccc atcctttgtt
     1981 gtccttatct tccattaaca cttcagaaac aaacatattt atctccttgt gggtacccag
     2041 agatatttta tgattgctcc cggtcagatc aataaagtgg cttcaatttt gccttaatga
     2101 tcaaatcagg gtgattgacg gaatcgtaca ccacctcaat attttcatcc gtggcgtcga
     2161 taagatattc ttttacataa ggacctgttg attttccatg aaatacatct tcaacaagta
     2221 cactctcccc ctgaacaaca cgaaaactaa cttctgtttc gaacggacca atcgtcacca
     2281 tcagtttttt cacatagcct cctgataagc actcgattta ttagttaatg gtgtaacgca
     2341 gatacaaaaa aggcccgcaa aagcgagcca gaaaaataag tatggcgcgt tgtactggat
     2401 tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgct
     2461 gaataccgat aatggaccgc catcgaggac tcgaaccccg cgcaaccagc ttcgaaggct
     2521 gacgctctgt cccgatgagc taatggcggt atgtgatatg gtggcccttg ctggatttga
     2581 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggtgc
     2641 aggataataa cggtacgtaa ctaatcctgc aatatcatcc gttctgactg gctaaatcct
     2701 gaacttccct gaccgtctgc tcaaaacgtt cagtctccag ctcaacacca gttgcacgac
     2761 gccccagtgc catcgcggct ttgactgtcg aacccgaccc catgaaaaaa tctgcaacca
     2821 ggtcccccgg acgactgctc gcgctgatta tctgctgcag catttctgcc ggtttttcgc
     2881 acggatgttt cccgggatag tactgcaccg gtttatgcgt ccacacatcg gtgtacggca
     2941 cctgcgccgt cacgccaaaa taccgccgca gatgcttata ttcactctgc agttccgcat
     3001 actgccggtt cagtgaagta tacgtctcca gcagctggtg gtggggcttt tccagtacac
     3061 cgcgctgatg cttctcttct gccacccggg caaacagcga ctgtaatttc agataatcgc
     3121 tttcgttcgg tagctgccac tgactggcac tgaaccagtg cgacaccatg tttttctttc
     3181 ctgtggcatc cactatctgt tttgccgtta tccccagggc agcacgcgca tcacgaaagt
     3241 aagcaatcag cggagccatc acatgctgtt tcagtgccct gcccttcgcc tcatacccgg
     3301 catctttcgg acgatacggc ccctgataat gttccgcgaa cagaatgcgc tctgtggcgg
     3361 ggaaatacgc cctcaggctt tccttgttgc acccgttcca gcgtccggac ggctttgccc
     3421 agatgatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg tcagacgcca
     3481 ggcgatgacc acagaacagg taaagacttc cggcaggttt cagcacccgc cagaactgcg
     3541 caagacactg gtccagccat ttcaggtaat cctcatcccc cgtccactgg ttatcccagc
     3601 cctcgggctt cactttaaag tatggcgggt ctgtgactat cagatcgaca gagttttccg
     3661 gtaaggtctg ataaattcca ggcaatcagc gttgattagc tcacaactgg atatttttac
     3721 agtattagcc atagatcaat aagcacttct ctgataggct cattctgctt ttgcgcaaag
     3781 cagtgggcct gaggtttgct tgtgatccgg acgcatgagc agatggctgg tgagtgcccc
     3841 taacacccac cagccgccca tttaccacaa ataaaaaagc cttcaggact gaaggcgtct
     3901 gtaacaaccg aactgatagt ctgccagccc cgccataaca agctgggtca gtattaactg
     3961 gcagcgttca cgtgaaaggt acgtattctg cgcaatctcc ccgactgtcg ccggttcggt
     4021 gacgcttaat tcattaaaca ccactctggc ggtttctgtc atatcctgct gtttcagcat
     4081 gtctttttcc cttttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca
     4141 agctgaaaga ccggtattca ccgccaccag cgcgtttact gtactggcgt gatttcagtc
     4201 ataaaaaaac ccgctcgcgg cgggtttaag ctgtgtggcg aagtaaccac tcttaacata
     4261 ctgacatact ttttgcggac cgcactaatc attttttact tttttagcag ccagtcgtcc
     4321 atctccagtc ttacccccag cacagacaaa catccgtcaa taaacccttc ggctatctgc
     4381 atctcaattc gtattgcttt ttcgcttttc tttctcgtcc tggctatctg tctttttgat
     4441 attcgcaaca aataatgagc aatgagaagc gaatactcct caggtttttt ctgcttcaga
     4501 cgagcaagac agttttcaat gataagtccg tcatcatcgc agcaggccgg acgtggttta
     4561 gtggcagatg gtaaaagtcc tttgaatccg gcagcgatcg gagaatagtc caccccggtg
     4621 ttaccacttg ccgcccatgc cccccagcgt tcaagaacca tctgaatatc acgcatcaac
     4681 tttctccaca aaatcaggac agcacaccaa tcgccagtgc gcgatcgata aaacgaaata
     4741 tcagctccag ttgggaacca tacttctctt caaatgccac agtatccgca tgcagttcgt
     4801 cgtggtgttt tctgcacaaa ggcagcacaa aaaggtcatg cgcttttgtc cccataccac
     4861 cctgaccgtg accaatcaga tgatgcggat catcggctgg ctttccacaa cacgcgcacg
     4921 gctgcgtctt aacccagcga gtgtactttt cattaaccca gcgacgacgt ttcgggcgta
     4981 acataaaaga ctccggcgac tccggatcca ctttcagcgc cagcaccttt ttcgctttat
     5041 cccggatgat gctggtggcg ggaaccgaag gcacaaggtc actttcccgg gtgacagacg
     5101 gcacaacagg cttcggtaat ctcagtgcct tacgggctgc actttccggt aaggcatccg
     5161 ccaggtcatt acgaaccagc caccagcaca gttccggcat tgtcacaacg tgactgtcat
     5221 caaaaccaag atcacggcgc acaacagaca acacccagcg ggcacagtta tccgttgcca
     5281 ttgcttccag ccgttccgtg aactggtcac ggagaagatt gtcacagtgc cagcacagac
     5341 ggattgcacc cggcgcgagc cgcattgtgg tcatgttctc gctgtgccat ccggaatgag
     5401 gccactgaca gcccttttca cgaagtaacc agctttcaag acattccacc ccaccagcac
     5461 gacgaataac tgcctcattg cggaacacgg cccgcacagc aggatcatcc gctaacggtt
     5521 gtgatgccgc cggaacggca ccactggcaa aagatgaata acgttccggc tcaggctcca
     5581 gcaggacacg cccctgcata aacaggggca tcagctctga gcctggtctg aacagtacga
     5641 tccccattcg cggggcaatt tcaggagtca acagtgctct cacggtcacc tcagcgaacg
     5701 atattgcatg aacacaggag aaaaaattca gccatcacgc agtaaactcc ttcaccagcg
     5761 tttcaaactg gcttaccttg ccttccagtt ccgccacgca atccaccagc tcatccactg
     5821 ccttttgtgt gcggtgtttt gcctgcagca gatcacgaag cgccggagta agctgcttgc
     5881 ggagcgtatc ctttgccacg ctcatttttt tccatctgtt cagcacaacg aagcatctcc
     5941 tgcgcctggc gacgaagttg ttccggtgaa acagtggctg ttctgttatt caaaataaac
     6001 gctccgcttt actgcccgac atgcggttat tgctgtatct gcgcggattg cccggcgtca
     6061 tgggtgtgga aagaacccgg gcactctcct ggtccacagg cagaaaatgc ccgttatgaa
     6121 aacgccggta aatggtaccc tgcgtgccat tacgctgttt cgtgatgttg atttctgcta
     6181 tgcctctcgc ctgtgtctcc gggttgtaca cctcatccct gtaaagcatc agaatgatgt
     6241 ctgcatccgc ctctatttcc ccggagtttt tcaggtccga gttcatgggg cgtttattgg
     6301 gtctggattc cacaccgcgg gagagctggc tcagcgctat cagcgggaaa ccgccggatt
     6361 ttgccaggct tttaagtccc tttgagattt cccccacagc aaggtcgtga cgccccgtac
     6421 tacgggtttt aatcaggccg aggtaatcga ccaccaccag cgccgtttcc gggtgtttca
     6481 tccggtggtg cctcgtggtt gcacatatct catcaatggt caggtttgcc tggtccacca
     6541 tccagatatg acgccccgtc attcgtccca cgccctgtga gaaacgcgcc cagtcttcat
     6601 cttcaaaacg ggcaacagac ttaagacggg ataccggcat tcccccggca gcagacacca
     6661 tacgttcacc aatctggatg ttcgccatct ccatggtgaa cagaagcacg ccatgcccct
     6721 gctcagtcac cttgtcgatg atatccagcg caagttcggt tttccccatc gaaggacgag
     6781 ccgcaatgaa taccaggtcg cctggctcca taccgcccgt ttttgcgtcc agttcatcaa
     6841 taccggtcat cagtgccctg gatttctcca gtccctgatt gcggcattca acacggtcga
     6901 ccacttccgg aaggacatca tcaatgtgaa ccggctgaat gacgcccttt ccggtcgaca
     6961 gtgaggtcat catgttctgc gcatccttca gggcatcctc agctgcttca caggtatgcg
     7021 catcacgtaa attctgtaat gcttcggtca gtgttttttc tgcatcgcgc agtgcggcat
     7081 tgcgccgcag cgctgcaaca tagtgctcca gtgaagactt cgcccaggtt ttgcgtccgg
     7141 tgtcggtaat caccggggca agttccggca tctcattgca cagcagtacg gggtcaatga
     7201 cgccggatac acgggcctgt ctgcaaattc ccgcgtaaat atccctgtac tgacgcacga
     7261 aaaagacatc cgccggaagt gtggccagaa tatccatcac ttccggatca gccccacgca
     7321 gaaaaaacgc gccaatgaca gctccttcca ggtcatcatt acgccacgcc ggatttgtcg
     7381 ggtttgtcat gctgccacac ctctgatatg cgcacggtag ctttcccaac caaacaccag
     7441 gcagttacgc ccaccatcag taacgcgatc cacaatccgt tcaccaatgg attccttaag
     7501 ctgttcaaac gtcaggttgc tgatcaaaat tgtcggtaaa acgctttcgt aacgcgcatt
     7561 tatgatttcc tgcaggatgg ttatctctgt aggcgtgccg aactgcacac caacctcatc
     7621 gatgataagc agatccagcg atgcaaaatg attaatcact tcatcgtcag tgcgctcaga
     7681 gttgtggcgc caggtatttt tcaccgcacg ggtaagccgc atcacatccg tgatttctat
     7741 ggttgcctgg tggtggcgaa taatattttt tgccattgat acagccaggt gatttttccc
     7801 ggtaccacaa ttgccaatca tgaccatgct ggtgcctgca gcgagacatt tcttccagga
     7861 agcggcatag cgctgacagg cttcaaggtt tttctgtgcg tcagtattca ccgcctgata
     7921 attctcaaac tcacagtcct ggaaccggcg ggcaataccg gcctgatcga gtaattcacg
     7981 aaccttcagg gcgcgtaatt cgtcatacac gcggtccagc tcctcactga ggcaggacag
     8041 gcaaccggac acccgtttga cagctttccc ccttacatcc gggcccgtca gcacatagcg
     8101 cgtgtatttt ccgtgtttcc cgcaggacac cgtctcagtg ctttgcaccc aatgctcaca
     8161 gcgccacgga cgctttccgc cacggacaaa tgccagctcc tcttccaggt ctgccttgcg
     8221 gttaagtaac tgcgctttgt cgtgttgcat gtgttgtttg ttcaggaaat tagtcatttt
     8281 caccctcccc acaaaatcac cagttgaagt tcgttgagcc gtagtcctgt tcactgaatc
     8341 ccgagatcgg gaggcttttg ccccgcccac ctccgggggc tgctggctgt tgccaggatt
     8401 cctcgaaatg acgatcgggt ccaaagaacg tcgacgcctg cttcacgaac tgggtaccgg
     8461 tatttccaga gacacgcacc caggcggcat agcgtttcac accgttgagc atggtttcgg
     8521 gtgtcacacc ttccctgatt cgggctttcc aggctttgaa ggctgctgac ttggaattac
     8581 caccagcacg tttgggatat tcctgccagg cctgttcaaa ttccggtgaa tattcctgtc
     8641 tggcagaacg cgctggtgca gacgcgtcag cggatgcgcc aatatcttgc ggttcatgtt
     8701 ttgaatttac tggtggttca tgttttaaac cttgtggatc tggggtcaga ttctgaaggg
     8761 tcaaacgcgt atttttgcca gaatctgaag ggtcaaacac acctgaacat acagattctg
     8821 acggttcaga ttttgaaggt tcagaatctg aagggtcacg taatcctgaa agtctgcgct
     8881 gctgtttcag ttctgcaacc ttatcccgct cagttctggc aagcagctca agccgatcag
     8941 cattcagatg ataaagattg gacgtattac ggttaccttt gcggcgtgac tgacgcgtca
     9001 gccagccatc agcctccagt tcggagattg ccgttctgac tgtactttct cccaacccaa
     9061 gctgtcggca tatggtttca acacctgggt agcacacccc gtcatcattc gaataatccg
     9121 ccaagcgcgc cataatcatc agttttgcac ctttgatgcc atatgctgca catgcatccc
     9181 agacattacc taggattttg ctactcatac ggcacctccc agacgcttaa acatttttcc
     9241 ggactgaaac gccaccagcg gataactcag ggtatgagta cgtccctgaa cctggcagac
     9301 aaccttctgg ctttctatat tgaccaggca aacccgcaga acgtgtccgt tgctggtggt
     9361 gaaccactgc cccacacggg ggcaatggtt gtatcggtga tacaggaaat taacgatgtg
     9421 gcggatcatg gacgcacctc cttgtcagaa ccattcagcc tggaatcaac aagtgcagca
     9481 ccaaaaacag catcacctac acggtcgtac agtttgctgg ccagcggaga ttcaacagcc
     9541 ttaagcattg gataaagctg gcttgtccag atttgatgga tttcacgcaa atgcaggtat
     9601 acgcctctgg cgtttcgtgc gacagctgac atatcagacg catcggcacc tgataaactc
     9661 ttctccatct ggttaaaggc attgatgtat gcctctttga accgggcagc acgtttacca
     9721 gtgaagccca tagcaagaaa cgcaaagccg tcgcgggtta tttgatagca aggaagtttg
     9781 cgagtaccgc cgttgggctg gcgtaccaaa attgatgtct ccgcaaaatt gcgggcacaa
     9841 aactctggag aacaatccaa aatgcggatc tttttcagaa catcgtcatg acgtttagag
     9901 aagaagtcag caacagccaa agaagatgta acagcctgac catcaacgat ggcaatttca
     9961 ggttgagaga gggttgggag agtagtcata gtgacagccc cggtagtcag ttttttagaa
    10021 aactcaccac atgggacgcc aatcacagag gtggtgagac gtacagggtt ggcgttaccg
    10081 gagactaccg aacccggccc gaccgaagtc ggccctgtac gccccaccat aatttgggcg
    10141 tagtaatgct catgacacga aaaaaccgca tgagcgcggt tatgctcagt aatcaatttc
    10201 aggacgccaa tcccggcacc cgttttataa ggtgccggaa cagtgtaacg tcccggactt
    10261 gcagaatcaa tatgctggtg gcgcgtcatt gatgcacctc ccgttgatta cgacggaaca
    10321 cggagtgacc ctggacggct tcagcctcat ggaatgcttc aatgcagctc tcgtagtacc
    10381 gcatggtgcg cagacttaac ccaagctgta gcatcatcag accatcacgg gtgatgtaat
    10441 aaccacgcac tgaatcgcca taggcgcgat aagtgccggg aataaagttg cgggtgagaa
    10501 attcgcgtga gcagttgaga tattcgattc gatcgatgat attcttatgc atgcgcttaa
    10561 agtgacaggc gacatacagg gagaatataa cggctctgcc gttgacgacc tcgattttga
    10621 ggtatggatg tgcggggatc gtagccatta aggcagcctc ctgtaacagt ggaacagttc
    10681 caccaccgga ggtctcaatc tcgctggtgg cggactggac agggttgaga ctaccggcgt
    10741 tacaggaaac cggcgaacct ttcggttccc ctgcccagcc caccataatt ctggcgtgca
    10801 aggacgctga caacaaaaaa gacgctggcg cgtcatttgt cgcctgtaac aattccgggg
    10861 tctcactccc gatagtggat tttgccacta tatgtaaagt gtgccgccgg aatttaattt
    10921 tgtcaacgag gttaaatata gagtcgctat gttggtggcg cttcgcactc aacaaaatca
    10981 cgacgaaaca accacagcgg gatgaagcat tcatgcggat aaccatcacg caggtaaata
    11041 acccgctgtg tttcagactc ccagcgtata acgtggacgc gacgccccct tccatcacgg
    11101 aaccagcgat tgagtacttg catgtgttac ctgtgagcat aattacacct gccagcccag
    11161 cgcctggaac aaccccattt tcgggtgata ccagcgagta cctcgtggtt ctgcttcact
    11221 cgtcatgcga tgaaaagcag acatgaaggg ttccacagca acaatagcgc gacgcgataa
    11281 caatccatcc ggcgtcatga actcatgggt gtcggtaggg atctgatatg cgttcaccag
    11341 attgcggcat ttcgcatctg acatacccgt tttcgccgcc agctgacgat agcctgcata
    11401 accatcgcgt atggcgcctc ttttgatttg ttcgacagtt tcggtaacgt gactgacttt
    11461 ttcttctacc cgctcaaggc gtttctgctg acgaacagct tcaagagcca tcgcggcaac
    11521 catttcgatt tcgctcattg gcttgcggat ctgttcttcc agttcgcgcc agcggtctac
    11581 cagacgggct gtgaattcag ggcaaagctg tgcgacaaca atgatgctgt cgcgtttgcc
    11641 ttgttcgcct tcaaacagat aatgctcgtg ttgaacttta aaccctaagt tattgattct
    11701 ttcggaaacc tcaatttgag gagatcggac aacaccacct ttagctaatg tttcaatagt
    11761 gcgtttcaca ttgtcatgac gcttacccac cagctctgcg atctcaacgc tggtcatgga
    11821 tgctttatcg gtaaaaattg ttgtattcac tgtttatctc cttcgcacac tccatcttct
    11881 gtgtgcgcta aacttgggtg tgggaaaagc tccggtagat caggacgaaa ttcatacgcc
    11941 tggactttcc catcgaccgc cttaaccaga gacggaacaa attcaggaga aatttttttc
    12001 ttcccattca gccagtcgca aattgttgac tgcgcgcgac cacaacgcct agccagagca
    12061 atctggcttc ccgcaatatc tacagccttg gctatacctt catttttcat atcgacctcc
    12121 aacctgattc cattcactat agcgattgat taaagcgatt gcaatgtacg ggaaagcggt
    12181 ttgtgtatat atcgctaagg cgatacacta aaagggcaga aaaaggagga cttatgagct
    12241 tttcagacag gctacagtta gctatgacta tccgcggata ctcgcaggga aaactggcca
    12301 gagaggttgg catggctcaa tcgagcgtaa ataaactggt taatggtgcc acaggctcca
    12361 ggaaagttgt ggagatagcc aataccctga acgtgcgccc cgagtggcta tcgtacggtg
    12421 ttggtcctat gaccccagaa gaaagcacat cctgcaatcc gacaccacag gtatcatatt
    12481 ccccgctgga aaatgataag gatatttttc gtgtggaagt cctggatatc gctgccagcg
    12541 cgggaccagg aacattcctt gtttcagatt ttgcagaaac ggtccatgct atagaatttt
    12601 cacatgacgc cgcaagacgt ctgttctgta gcaggccagc aaatatcata aagatgataa
    12661 cagttgatgg cgacagcatg gctcctactc tgtgtgcagg agatcaggtg tttgtcgacg
    12721 tatccgtcag aaattttgaa actgacggta tctatatttt tatttttggg cacactttcc
    12781 atatcaaacg cctgcaaatg caagggatgc aacttgctgt tatatcagac aatccggcct
    12841 acaaggaatg gtttataagt gaatcagctg aagaacatct attcataatg gggaaagttc
    12901 tcattcatca gtcaatccag tataacaggg tcggataagt caacatcacc ggcagctgta
    12961 ttgcgcagct gctctacttt cctgtatctc aactccctac gtatcgtctc atttctctca
    13021 taatctgttc tttgctgccc aaaatgcata tctatagcga ttgacataac caaacgcttt
    13081 ggctattgta aaattaaaac aacaggaggc tcatatgaag catgcagtta aggtgatgga
    13141 tgaaattcag cacaaataca agaaagcggc tcgctgcttg gaatgatcta ttcctacatg
    13201 tcagaaaacg gagcagaaag tgagagggtt tataatgcaa tcagatgcct gatgcgttcc
    13261 ctgcaaaaaa cagaagaaac tgcatcaatt tttgcagaaa aactatgtga ggcaaaagct
    13321 atggtttcgc atggtcactt ttttggaaaa atccgtgccc aacgtgaagc ctgcggcttg
    13381 acgaccaccg aacttgccaa actgcttgat ctcgatgaag agatcatcct gcaatgggag
    13441 agcggagagt tcgaaccgac catcagcatg cttatccccc tggcaaacgt cattgggtgc
    13501 gatccgcttt ctttgctgga tgaaaaagat agcgcgtctg ttattcgcgt aaatgcgcct
    13561 gaagtctacg tggaaagtat tggcgcacgc atcaaaagcg cacgtaaaaa actgggctta
    13621 acggaagctg atcttgcccg catgattaat acctatagcg accccataaa cgactgggaa
    13681 tgcggcatct gtgaagttcc tgctgatcag atagtgccac tggccagtgc gcttaattgt
    13741 gacctgatgt ggttgttaac gggaaaatca gaagcaaagg agtagcaaca atgactggta
    13801 atatccatga taagtatgaa ggcttatgcc tagcaccaga ttccttcgca aacaatatcc
    13861 atgatttatt atgcgcagtt gtcgtattac aaatgtcaga caacgacgca ataaaaagaa
    13921 caggtgatga agttcttgaa tttgcacgtt gctatgctga agcagcagct gaaaaagaac
    13981 tatccagtta aatagaacaa gtcatctcca gatactatat tacggcttaa tcgccgggga
    14041 ttatcacaca cttaatccac aggaggtttt attatgactt ttataaaaca taaggcatca
    14101 cacaaaacag cctgccttat tgcacaacac gggaaaaatt acatgcatat tgcctgcttg
    14161 tttctgcgta aagcatacgg gagataataa tgcatcagaa aacagcagaa cacgaacaaa
    14221 ccagagtatt gctgaccatc aaaaacggga aagtaatatt aattcgtcat gttcatgacg
    14281 atgaatttgt aggaaatctt tcaacattcc tgtttattgc agaaaaggca ggatatgacg
    14341 ttattgcacc agcagatgaa gatgaggaat aaatttcatg caatacgctg aattccaggc
    14401 tgaagcaaca gccaatggta tacaaacagg cagtatgact attgattatc acgacgccat
    14461 acgccgtctg gatgctggag aattcgatac tcctaatgtg cgaggtttac gtatccttca
    14521 gtgtctcgcg caagctgacg aagcaggatt actgggaaaa cttccggttg agatgaaggt
    14581 tgcccagtgg cgatggttgt atgtgacgac attcatcaac gaagaagaag acaagaacgg
    14641 cacaattgat gtcccgaatg aacacggaac aacagatcgc gccgtaatat ataacgggaa
    14701 gcatggagtt atgactatat accctagtcc cattcggttt gccttacagc agtatattga
    14761 atggaattta attcaaacat atggcgaagc tgagggaatg ggaagagcgc tgtttcttta
    14821 tcagaaaatg ctcaccactt cccctgataa aggtttcatc ctttcagata taggtcgaga
    14881 agggcttgaa ctccttctgg atgaaatgat taacgacctg aatactcatg gtatgccaga
    14941 aggccagtga cacattaaat attaagaaga atataactct tccgtttttt actaaccgtt
    15001 tatatgaaaa gcgaccgtga attaagcaga gtaaaactgc ttttaatcct tgccacagta
    15061 ctgacactaa cagaaatcat tattctcttt attgcgctgt ctgtcagtta aaaatatcgg
    15121 gataccacat accaatgaga ctgtatttca caatagtaat tttactggca attatcgcat
    15181 gcatttacgg attactcgtt ccgttcctta tatccatgaa ggatacgaga gcagttattt
    15241 ctggctttgc actggcgttt ctgaccccgc cctgcattta tgccatttac aagggtcttt
    15301 ctttcactaa ggataaaaaa tgaaaaaaat tatttttgct ttagccattg ttctgccgac
    15361 cattggtctt gtcggttgcg atcgcgttga gcccggtaat gttggcatca aagtaaataa
    15421 actgggcgac gataaaggcg tcggtgaggt ggtcggtgtt ggtcgctact ggactggctg
    15481 gaatactgaa gtttacatct tccccacctt caagcaaatg aagacctacg atgaaccgtt
    15541 cagctttcag atgagtgacg gcacaaccat cggctatcac atcggtgtgg cctacaaggt
    15601 tgatccatcc aaagttacta cagtgtttca aacctaccgc aaaggcgtgg atgacattac
    15661 cgacactgac ctgcgccaga agatagccga cgcactcaac cgactggcca gcaaaatgac
    15721 caccgataaa tttatcgacg gtggcaagtc tgaactgctg gattcagcac ttaaagatat
    15781 tcaggcagaa atgacaccca tcggcattca ggtaatgagc ctctcttatg tcggtaaacc
    15841 ggaatacccg ccaaccgtga tcgacagcat taatgccaaa gtcacagcga accagaaaac
    15901 cctgcaacgc gaacaggaag taaaacagcg cgaagcggaa gccaacatgt tgcgcgcgga
    15961 agcagccgga caggctgacg ccattcgcac aaaagcccag gccgaagccg atgctattcg
    16021 tttacgcggt gaagctctgc gccagaaccc cggtgttatg gagctggaag ccatcaataa
    16081 atggaacggc acactgccgc aatacatgac cagcgatgcc aatacaccat ttatccaggt
    16141 taaataactt atatgcccgg caggccgccg ggctaaggga aaagcagatg aacacccata
    16201 atgcccaacc gcaaataatg aactatgacc cgaatctgac gtcatgcgga cgcatggcaa
    16261 aacaaaccgt tcgattaact ttcgggttat gggaataccg cgaaacattc gaagttactg
    16321 tcggcggcaa tctgaccgga ctggatgtta tcagttgcgc tattgaaagc ctgtacgcaa
    16381 cactgcctta tgaagaagtc gaggatgagc gcacaggggg aacagatatc atggccacca
    16441 ttaatattgg cgaactgata tgtcaggatg aagacctgtc cggagagagc tggcttgctg
    16501 gaatgcttgt agctgcagaa attatcagta ttgaacccgc tacaaatata cggctctgaa
    16561 gttctcgcta ttcagagaac aggagaaaaa atgttcgatt tgattaatca aggacaactg
    16621 tataccgaca gcgccggtta cccggtaaaa attattcact gcataaataa catcgtgttg
    16681 tacagaagaa tggatggacg aacacagtcg gtaaaaataa acgattttaa tgaactgttt
    16741 gaacgaatcg atcaccagga ataccgacaa attctggcag aaacagaaca agaagctcat
    16801 ctgaaaaaat tacgtgccat gaaaaggaag taaagaatga ataaagcgtt tgagctatgg
    16861 gtacgccagt gttacggcaa tcgctatgac ctgacgcgag atgttgacgg tttctactgc
    16921 cgtgaagttg tgaaacgaat gtttgacgtg tggtgccact gccgtggatg aaaattttat
    16981 gaggttggca tgcagacaat catctatcag ataaccccca gcaaatggtg tacggagaga
    17041 gtcctcattg catcaacagg gctaaagcct ggcaccattg agcgggcaag aagaaagtca
    17101 tggatgcagg gaaaagaata ccgccattac gctgtagaag gtgatccagg gcattacagt
    17161 gaatgcctgt acaacatcga agaaattatg cgatggatcg aaaaccagaa acaaccaggt
    17221 gtcaaaaatg caagttccgg ttaacctgtt aatgctcctg gacgtctggg aggttcaatg
    17281 agtaacacat catacccgac aggcgttgaa aaccatggcg gatcactccg catatggttt
    17341 cactataacg gcaaacgtgt cagagaaaac ctcggtgttc ctgacacagc caaaaaccgg
    17401 aagattgcag gcgaactcag gacttcagtt tgttttgcaa tcagaatggg gaatttcgac
    17461 tacgctgcgc agtttcctga gtccccaaat ctgaaacact ttggtctggg aaaaaaggag
    17521 ataaccatta aggcgctttc agaaaaatgg ctggatctga aaaaaatcga aataggaggt
    17581 aacgcattca atcggtatca atctgtggtg agaaacatgt tgcctcgtat aggggaaagc
    17641 cgactggctt catctgtaac aaaagaagat ttactcttca tcaggaaaga tttattgacc
    17701 gggtatcaca atctctcaaa cggcaaaaca acacctgtta aggggagatc tgtagttaca
    17761 gttaattact acatgacgac aattgcaggt atgtttcaat ttgcagctga caacggctac
    17821 acggaatcaa acccgtttaa cggcctgaca ccattaaaaa ggtccagaac cgagccagat
    17881 ccgctcacgc atgacgaatt cattcgtttt attgatgctt gccaccatca acaaacgaaa
    17941 aacctgtggt ctttagcagt atacacaggc atccgtcacg gtgagctaat atctctcgcc
    18001 tgggaggata ttgatttaaa agctaaaaca atgactatcc gtcgtaatta tacaaaactc
    18061 ggggagttta ctctaccaaa aaccgaagcg ggaacagatc gtgttattca tcttgttcaa
    18121 ccagccattg aaaccctgaa aagtcaggct gaaatgacaa gatttggcac tcaataccag
    18181 attgacgtca aactgcggga gttcggacgt accgtaagcc atgaatgcac gtttgtcttt
    18241 aatccgcaac tggtgaaaaa gtgccagcaa gccggttacc actacaaagc ggactccatc
    18301 agggattcat gggcatcagc cctgagacgg gccggactac ggcacaggaa agcctatcag
    18361 tcaaggcata cttatgcctg ctgggcactg gccgcaggag cgaatccaag ctttatcgca
    18421 aaccagatgg ggcacgcaaa cgctcagatg gtattcaatg tttacggagc atggatgaaa
    18481 gataacaata tcgggcagat agaactgctc aataaggagt tagctgaaag tgtcccatac
    18541 atgccccaca gagcaagacg ctgaaataat ttctttcagt atcattgagt taacccatgg
    18601 ttcccgccat gttaactgcg tggagggcaa caccacgctt tacgccctgc cgaaacccga
    18661 ggttgtcctg cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc
    18721 ggcgaccatt tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt
    18781 gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac tgcctgccac
    18841 attcggccca cgggagctgc ctgcgctttg gcattttctc gattctctcc ctggcgaatt
    18901 taattatggt gtggaagtcc gccatccaca gtttttcgcc aaaggggaag aggaacaaac
    18961 gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt
    19021 tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
    19081 tccggtacat gctgtactga cggcgacaaa tccactgatc cgttttatcg gtagtgatga
    19141 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
    19201 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
    19261 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
    19321 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
    19381 ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa
    19441 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
    19501 gtggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
    19561 ccatcgcgat tgtgttgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
    19621 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
    19681 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
    19741 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
    19801 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
    19861 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg
    19921 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
    19981 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
    20041 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccattgac
    20101 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
    20161 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
    20221 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcgtt actggataaa
    20281 atttatcaag ggctgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
    20341 gatgccaaag ttggtgaact gctgttcaac atgcaccacg actttaaacg tgccaacggt
    20401 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
    20461 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
    20521 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
    20581 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
    20641 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggttgttt aagcagtggt
    20701 caaatgcggt ggagtttcta cctgaaatta aaccgtatcg tcttgattta ttgcatagcg
    20761 ttacggcaga aagcgaagag ccactgagcg cagggcaaat taaacgcatt gaaacgctga
    20821 tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
    20881 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
    20941 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
    21001 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
    21061 cggtgcgtaa actactgggt aatgatcagc gcgcgcattt gttaccgtta ggtattgaac
    21121 aacttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt tatcatcgtc
    21181 gttcaccgct ggagcatctc tggcagttga aagatcaact ggtcaatgaa ggtgaactgg
    21241 tgcttgaaac gctggttatt gatggcgatg agaacacggt gctggtgccg ggcgatcgtt
    21301 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
    21361 agaagtgtgg ttttgtagat atccgcattg cggatgtgag cgttaccact acagaagagc
    21421 agcgacgcac tgaatggatg gttaccgagt ctctgtccga tttcctcgac ccacatgatc
    21481 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
    21541 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccagc ctacaaaacc
    21601 ttgctaattc aatatattgc agggactatg taggcctgat aagcatagcg catcaggcag
    21661 ctttatgttt gcataacctc agcgcccgtt tccgggcgct tttcacgtct tacgcctgaa
    21721 cagctggctg atcaaacgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
    21781 ccagcgcaga gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta
    21841 acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat
    21901 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcacctgtc agaatctgac
    21961 cacgtttgtt ccagacacgc accagatcat cattcgcaat accaaagcga gcagcatctt
    22021 cggtgtgaat agtaattggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
    22081 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
    22141 cgtcggcggt acccttccac tcatcaggcg caagccaggt cgggtgtgcc gggcaatcct
    22201 tatagccaaa tttctccagc gtttttgagt agatttcaat tttgccactt ggtgtaccca
    22261 gcgcattttt caccggatct gcgcggaaat cgccatagcg aacgaactgc tcattcttct
    22321 cgctgcggcg catttcaagc aatttatttt gctgccagaa ggcattaaac atcggcatgg
    22381 tgacgcgttg cgcacgcgca cctttctgag cggcatcata gaaaaatttc agccacgcca
    22441 tttcatctct accttcggta tagatctctt ttccgcctgg tttcagtaat tccgcaaggt
    22501 cggcaaacac gtcaaaatcg ttgcgtgctt caaattgcgg agcgacaacc tgcttcatcg
    22561 gcacaatatg ttggttactg taatcgccgg tcatcgtcag atcattgcct cgaacgatgt
    22621 ggtgatcggt aatacgatat ccgcgtgttt agctgccgcc gtccagtagc actcagaaac
    22681 gacgatcatc tccggtttct gccaggcttt aatcagacga ttagtatcct ggtgatgggt
    22741 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
    22801 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
    22861 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
    22921 ttccggcagc acgccgccaa cacgcgtcgg gttgccaccg ttggagtaat gataagagaa
    22981 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
    23041 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
    23101 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
    23161 ttccgccgcc caggccgcgc tcttcggagt attatcgctc ttacccgtca gatactcttc
    23221 aaactgcgga tacccggtgg tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
    23281 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
    23341 ccaggtggca ttatcgccaa agaactcgat ggtttcggag cggataggat caatggcaat
    23401 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
    23461 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
    23521 gttttccaga atcagcggcc aggaggtctg ctgttcatac acctctacag aaccgaccac
    23581 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
    23641 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
    23701 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
    23761 atgttgctca tgaatcagtt ttaatgcttg ctcccagctc acctgtacat aggtatcttc
    23821 gcctcgacct ttcgccggtt gcagtggatt atcgagatag ctttttctca ccatcggatg
    23881 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
    23941 ggttttcgcc aacgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgttcat
    24001 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcgggtag
    24061 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
    24121 acgtcttgtt aatgtcataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
    24181 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
    24241 ggttcgtgcc cccattcctt tggcaatgga aggccacgca ttgacggtgt aatggtcggc
    24301 ggcaataggg gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
    24361 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
    24421 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
    24481 ttcttcactt aatgtggcta gcgccagccg ttgacctacc gccaggtaga gcgtgttttc
    24541 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
    24601 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
    24661 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
    24721 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
    24781 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
    24841 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
    24901 taattctttc catactgttt cggccatttc ctggcgatga gcttcgaact tatcgtcact
    24961 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcgaa
    25021 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
    25081 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
    25141 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaagaaccc
    25201 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
    25261 catagattcc tctaattaaa ttccagaaca ccgaaatcat tgtataaata aatacacaac
    25321 gaattgacta cttgcttaat tcggcacaac actgcctgaa ataatcgcta aagaatatga
    25381 ttt
//
LOCUS       DAZGCQ010000072        25024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000072 DAZGCQ010000000
VERSION     DAZGCQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.072"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHK07_003254"
     CDS             complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHK07_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO8103544.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQILKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHK07_003255"
     CDS             complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHK07_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8103545.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHK07_003256"
     CDS             complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHK07_003256"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO8103546.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3142..3678
                     /gene="yniB"
                     /locus_tag="ACHK07_003257"
     CDS             3142..3678
                     /gene="yniB"
                     /locus_tag="ACHK07_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO8103547.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHK07_003258"
     CDS             complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHK07_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO8103548.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHK07_003259"
     CDS             complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHK07_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8103549.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHK07_003260"
     CDS             complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHK07_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO8103550.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6292..7050
                     /gene="ydiY"
                     /locus_tag="ACHK07_003261"
     CDS             6292..7050
                     /gene="ydiY"
                     /locus_tag="ACHK07_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO8103551.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7103..7210)
                     /gene="yniD"
                     /locus_tag="ACHK07_003262"
     CDS             complement(7103..7210)
                     /gene="yniD"
                     /locus_tag="ACHK07_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO8103552.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7385..9283)
                     /gene="espL1"
                     /locus_tag="ACHK07_003263"
     CDS             complement(7385..9283)
                     /gene="espL1"
                     /locus_tag="ACHK07_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO8103553.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDTPTPSSEPINHFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLMQTKSANETPG
                     LYISMLYGFDEIIDIFLNTLTAPIAQKHLNKKMVMDILAMKTRDGEPGLFAAMENNHP
                     LCVTRFLSKIYGIAVKYNLSKINIIDLLKGATVHGTPALYIAMSKGNKDVMLSYISTL
                     DTFAKKYSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            9806..11734
                     /gene="thrS"
                     /locus_tag="ACHK07_003264"
     CDS             9806..11734
                     /gene="thrS"
                     /locus_tag="ACHK07_003264"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO8103554.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            11738..12280
                     /gene="infC"
                     /locus_tag="ACHK07_003265"
     CDS             11738..12280
                     /gene="infC"
                     /locus_tag="ACHK07_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO8103555.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12377..12574
                     /gene="rpmI"
                     /locus_tag="ACHK07_003266"
     CDS             12377..12574
                     /gene="rpmI"
                     /locus_tag="ACHK07_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO8103556.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12627..12983
                     /gene="rplT"
                     /locus_tag="ACHK07_003267"
     CDS             12627..12983
                     /gene="rplT"
                     /locus_tag="ACHK07_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO8103557.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13100..13224
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13106..13150
                     /gene="pheM"
                     /locus_tag="ACHK07_003268"
     CDS             13106..13150
                     /gene="pheM"
                     /locus_tag="ACHK07_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO8103558.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13433..14416
                     /gene="pheS"
                     /locus_tag="ACHK07_003269"
     CDS             13433..14416
                     /gene="pheS"
                     /locus_tag="ACHK07_003269"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO8103559.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14431..16818
                     /gene="pheT"
                     /locus_tag="ACHK07_003270"
     CDS             14431..16818
                     /gene="pheT"
                     /locus_tag="ACHK07_003270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO8103560.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            16823..17122
                     /gene="ihfA"
                     /locus_tag="ACHK07_003271"
     CDS             16823..17122
                     /gene="ihfA"
                     /locus_tag="ACHK07_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO8103561.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17223..18203
                     /gene="btuC"
                     /locus_tag="ACHK07_003272"
     CDS             17223..18203
                     /gene="btuC"
                     /locus_tag="ACHK07_003272"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO8103562.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            18266..18817
                     /gene="btuE"
                     /locus_tag="ACHK07_003273"
     CDS             18266..18817
                     /gene="btuE"
                     /locus_tag="ACHK07_003273"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO8103563.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            18817..19566
                     /gene="btuD"
                     /locus_tag="ACHK07_003274"
     CDS             18817..19566
                     /gene="btuD"
                     /locus_tag="ACHK07_003274"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO8103564.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19645..20109
                     /gene="nlpC"
                     /locus_tag="ACHK07_003275"
     CDS             19645..20109
                     /gene="nlpC"
                     /locus_tag="ACHK07_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8103565.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            20356..21069
                     /gene="ydiV"
                     /locus_tag="ACHK07_003276"
     CDS             20356..21069
                     /gene="ydiV"
                     /locus_tag="ACHK07_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO8103566.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVARLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIKAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21132..22568
                     /gene="selO"
                     /locus_tag="ACHK07_003277"
     CDS             21132..22568
                     /gene="selO"
                     /locus_tag="ACHK07_003277"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO8103567.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSNSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(22572..22763)
                     /gene="hemP"
                     /locus_tag="ACHK07_003278"
     CDS             complement(22572..22763)
                     /gene="hemP"
                     /locus_tag="ACHK07_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO8103568.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(22895..23941)
                     /gene="aroH"
                     /locus_tag="ACHK07_003279"
     CDS             complement(22895..23941)
                     /gene="aroH"
                     /locus_tag="ACHK07_003279"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO8103569.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24098..24931)
                     /gene="ppsR"
                     /locus_tag="ACHK07_003280"
     CDS             complement(24098..24931)
                     /gene="ppsR"
                     /locus_tag="ACHK07_003280"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO8103570.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         6382 a         5865 c         6412 g         6365 t
ORIGIN      
        1 aagtaaggca acggccccta tacaaaacgg accgttgcca gcataagaat taatggtgtg
       61 ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc
      121 tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga
      181 taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcaggca
      241 gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggagctaa
      301 cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg
      361 taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa
      421 tggtcgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc agacgacgcg
      481 tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg
      541 tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca
      601 gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc
      661 ccagtttgat gatgtcttgc aggttagaac ctgcaaccac tttggtcatt agtgccagaa
      721 cgccgtaagg ggtcaactgc atgaccaggc gaaccagttt catcacccag ctttgcaggg
      781 tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc agtttcagcg
      841 cagctacacc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc
      901 cggtcagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca
      961 ggtcagagac tttaccaaca tagttacttt cgatggcgtt cagacgtgca gtttctgcac
     1021 caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc acaccgacca
     1081 gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg attttgccta
     1141 actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag actaacggca
     1201 taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac cactgtacag
     1261 aatctttaag tatctggctg tcagaaccat aaatggtatg cagggcaagg ccaaaaacca
     1321 cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct
     1381 gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca
     1441 tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa
     1501 gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt ccagaaaggt
     1561 gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca
     1621 aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat gaatggggca
     1681 tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg ttaccctttt
     1741 ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg
     1801 cgggtgtcag catatcggca agtatgtggc ttaaataacc cagcaccatt ccttgtagcg
     1861 catcagccgg aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc agcagcgcaa
     1921 ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg
     1981 atatccactt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac
     2041 acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca
     2101 gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg
     2161 tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat acagtgcacg
     2221 gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca gttgaatgca
     2281 aattcagata aagaaccagg ggcagcgtga tcactgcccc tggtcattaa ccgagaaggt
     2341 cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat
     2401 cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaggcga
     2461 tcatgccatt taccgaatcc tctagcgcta cgcaggtcag tgggtcaacg cccagttttg
     2521 ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt tcggccgagg
     2581 cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc agcatatgta
     2641 gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac gcaacggctt
     2701 cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa
     2761 cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg taccaaagat
     2821 cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat
     2881 ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga
     2941 taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa
     3001 cctctccttt agagcattat gtgtaaggta ttaagctgtg cttatgatta agcgcctatc
     3061 atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag tgaaacgacg
     3121 cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa
     3181 aggattatgg gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg
     3241 aaatttatga atacccgaca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt
     3301 actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct attctggtat
     3361 aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc
     3421 ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg ccaggcgcgc
     3481 ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg
     3541 ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat tttcctgcag
     3601 tttttctcac tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct
     3661 ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc aacccatatc
     3721 atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga ccgccaaata
     3781 accttgctcg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg
     3841 caggtagcgg ggatactgac tgatagccgt cataaatttg tggcggctgt tcagtatgca
     3901 gcggtaacat cgccaggtcg cactctcggt caccccagta gcatgccggg tcgaaaatgt
     3961 acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag
     4021 gctgcggttg atgtgaggcg agacgctgct gaatatgctc gacgatggcg tcgatattgc
     4081 cgaaagcgat ccctttttct gctgccagtt ccaactgcca gccaatccgt tgttcagcaa
     4141 aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgcgttat
     4201 cgaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca atttgctgac
     4261 caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc atcaccagaa
     4321 aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg gttttactac
     4381 gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat
     4441 cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca tggacttctc
     4501 cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg ctcaaaagac
     4561 gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg
     4621 caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa
     4681 ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt
     4741 tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct tcactttggg
     4801 tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca tcatccgtca
     4861 tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg
     4921 tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg gccatagttc
     4981 acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg
     5041 gtgatgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt
     5101 ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc tgcactcccc
     5161 gcagctacgc caaaacgaac catctcttca agagaggcat tttctgccag tttcagtgtc
     5221 atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac cggtggtggc
     5281 accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc cagggaaacg
     5341 acaacccgtt tggccttgcc gctattaacg atttcttgcg cggctttgcg gacatcgtct
     5401 ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg cttaaccaac
     5461 tcgatgttac caatcgccag tgctgcactt aacgcttcgc cagaactgtc gatgatgcag
     5521 cggatccctt ggttttgcgc agcggaaatc agttgggtta atttttccag cttcacacct
     5581 ggcggcaggc ttccgcttat gaccaggatg gccccggact caatttccag aacttgctct
     5641 tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc
     5701 tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca
     5761 gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc ggtcgcgcca
     5821 cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg ggcgacgttg
     5881 atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc ttcgggataa
     5941 atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt
     6001 accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg aaagtgaaga
     6061 aattaacaat atgatttatt ggagctttat ttaaaatttt gcagataaat atatataaat
     6121 aaaaatctgt tttcgccaca tgcttattca aaaaatgaaa taagaataca ttgctaagtt
     6181 attcgcgcct ttttataatt cgttaccttt ccagggacgc taatctttga tccttaagaa
     6241 agtttccggg accgcgatgg tctctttttt tgttgtacgg actcttgata aatgaagctt
     6301 ttgaagacag ttcctgccat cgttatgttg gcggggggca tgtttgcgtc actgaatgca
     6361 gctgccgatg attccgtttt tactgtcatg gacgatcctg ccagcgctaa aaaacctttc
     6421 gaaggtaatc tgaacgcagg atatcttgca caatcaggca atacgaaaag ctcctcactt
     6481 acggctgaca ccaccatgac ctggtatgga caaaccactg cctggtcgct gtgggggaac
     6541 gccagtaata cttcttctaa tgatgagcgt tcttcagaaa aatatgcggc gggcgggcgt
     6601 agccgtttta atttaactga ttatgactat ttatttggtc aggcaagctg gctgacagac
     6661 cgttataacg gctatcgaga gcgcgatgtg ttgaccgcgg gttatggtcg tcagtttttg
     6721 aatggcccgg tacacagttt ccgctttgaa ttcggtccag gtgtgcgtta cgacaaatat
     6781 accgacaatg ccagtgaaac tcagccgctg ggctatgcct ccggggctta tgcatggcag
     6841 ttgactgaca acgctaaatt tacgcagggt gtttcggtct ttggcgcgga agacacaacg
     6901 ctgaactccg aaagtgcatt aaatgttgct attaatgaac attttggact gaaggtggct
     6961 tacaacgtca catggaactc tgaacctcca gagtccgcac cggaacatac cgatcgtcgg
     7021 acaaccttat cgctgggcta cagcatgtaa aattatctgc cggatcgtgt tatgcggtct
     7081 ggctgctttc gtttgtcact gctcagcccc aaatcattgc tgcccaacgt agcaacaaca
     7141 tagcgccaca gattgccagt agcaccacca ccatcttcca gtgtttttta tcaaagcgtt
     7201 tagtcggcat accagtgtcc tgcatatctc ttgtgcgtga taacggagtg taacaaaagg
     7261 gacaggcatc aagggatttt ttgttattac agccgtcggg tggcgttgta cacctgatca
     7321 ctgtttctgc gttttatgaa aaaataaaat tatgtatcga tatataaata tcgtaaatag
     7381 ctggttataa atacgtcttt aattcatcag cactaaaact catgctttgg ctgattacat
     7441 ttatagcagc ataatatgtt tctacagttt tataatgatt atgatgaatg gctatatgaa
     7501 cagctgacat gttgtcatga tttttagcgg ccaacagtgt aaataactga tgttggctaa
     7561 aagaatattt ttttgcaaaa gtatccagcg tcgatatata agataacatg acgtctttat
     7621 tacccttgct catagcgatg tataaagcag gggttccatg tactgttgcg ccttttaata
     7681 aatctatgat gttaattttg ctgaggttat atttaacagc gattccataa attttagaga
     7741 ggaaccgtgt gacacacaac gggtgattat tttccattgc agcaaataac cctggttcac
     7801 catcacgtgt tttcatggct aaaatatcca tcaccatttt tttgtttaaa tgcttttgtg
     7861 ctattggagc ggttaatgta ttcagaaaga tatcgattat ctcatcgaat ccatacaaca
     7921 tggagatata aagtccaggt gtttcgttag cacttttagt ttgcattaat ttaaccagat
     7981 tatcttcatg aatgatgtga ttatctacca gtgactgaat ttcttgcata aatattttca
     8041 cgttgtcagt atgtccgttc atcatagcca aaaataaagc aggtgtacca acgctattct
     8101 gcgcagttat tatttcaatg atttcttttt cagggagtat gggcaactgt ctgaataatg
     8161 taataagctg ttgagtctgg cctgtggcca ttattaacaa cattgccgat ggatgtatag
     8221 gggcaaatgg cattcgtgtc gcgatacccg tgaaaatagc ccctggaact ccctcaggta
     8281 ataggggcag aatagcaatt gcacagttgc gaacatacct atcagcccag gtctgagcat
     8341 cgggacctga actgatgaac ttatcaagcg agagaaagcc ccgtgctgtc ctgtgggtgc
     8401 ctctgacggc aacgttggta tcgttcgggt cgtaaacgga cacaacataa tttaattgcc
     8461 catacgccga ctgttttatt cgtagcctta ctgacaaagt atgattgtct atatataaaa
     8521 tcgcagacat atgactgaca ttgtttgcca tcattcgaca gaagaaaata tctaaagcga
     8581 agccaaaatc cttactcgcg ataacgcgac tccctgcagg aggatggcga agtaatgctc
     8641 tgaactccgc gtcccggact aaggggacat gctgctgtat ggattcaacg gaggagtaat
     8701 gtttaatcag gttgaccttg ccttttccat ttctcaatga atcaagtgcg tattgcaatg
     8761 acagatggcg acaaaaaata gcgtcagaag gagattgcga aaaaaatgca ctaccattga
     8821 gatttactgt tggttgtata gttttttctc tatggatttt tttagacagt ttcatacaat
     8881 ggttacgatt ttcggcagtt aactgttcct gttcaatata atccagtatg cttcgcaaat
     8941 ggttagcttt aataggcgtg gtttccagta ataaggaaat gatttctttt ttatagtgta
     9001 atgtaataag tatattttct gcatggatgg gtgaagtggc aaagttaatg gtaaaaaggg
     9061 cagtggaata acaattttgt gatatttttc gacagtttgc ggtaaattcc tgagtaacac
     9121 agtatgacag catcgaactt aagtcagagg tatcaagcag ggtgatgagc tggcgcgcaa
     9181 aatgattaat aggctctgat gaaggcgttg gtgtgtcgct atcgatgtgc attctatgag
     9241 gagacttata atgagttctg ataataatgt cgttttgact cataatcttt tcgccttata
     9301 tatgtctgga ggttttgagg atgatgatta attattatcg ccagaacgaa atgtgcacga
     9361 aaaattgcta taagaattgt gtcactttta tcattgtgat atgaccactt cgtcattaat
     9421 ctcatgatcg tttaaatact accgccaaat catgcaacga actttgctgg atttagttta
     9481 tgctgctgta aatccgctcg cagtaaacct ttcagacgct ccgtgatgtt atcagttgtt
     9541 ctttaagcgt tttgctggtg tactcactac aaacgaattg cgaatcgaat caatgtgaaa
     9601 cggaaaggta caatctccct ttttttagtg ttaacatcgc tcaaccgggt ggaaggtgag
     9661 aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga
     9721 tggtttcgca atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg
     9781 tcaccactgc aaataaggat ataaaatgcc tgttataact cttcctgatg gcagccaacg
     9841 ccattacgac cacgctgtaa gccccatgga tgttgcgctg gacattggtc caggtctggc
     9901 gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt gatgcttgcg atctgattga
     9961 aaacgacgca caactgtcga tcatcaccgc caaagacgaa gaaggtctgg agatcattcg
    10021 tcactcctgt gcgcacctgt tagggcacgc gattaaacaa ctttggccgc ataccaaaat
    10081 ggcaatcggc ccggttattg acaacggttt ttattacgac gttgatcttg accgcacgtt
    10141 aacccaggaa gatgtcgaag cactcgagaa gcggatgcat gagcttgctg agaaaaacta
    10201 cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt gaaactttcg ccaaccgtgg
    10261 ggagagctac aaagtctcca ttcttgacga aaacatcgcc catgatgaca agccaggtct
    10321 gtacttccat gaagaatatg tcgatatgtg ccgcggcccg cacgtaccga acatgcgttt
    10381 ctgccatcat ttcaaactaa tgaaaacggc aggggcttac tggcgtggcg acagcaacaa
    10441 caaaatgttg caacgtattt acggtacggc gtgggcagac aaaaaagcgc ttaacgctta
    10501 cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt aaaatcggta aacagctcga
    10561 cctgtaccat atgcaggaag aagcgccggg tatggtattc tggcacaacg acggctggac
    10621 catcttccgt gaactggaag tgtttgttcg ttctaaactg aaagagtacc agtatcagga
    10681 agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa aaaaccggtc actgggacaa
    10741 ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt gaatactgca ttaagccgat
    10801 gaactgcccg ggtcacgtac aaatttttaa ccaggggctg aagtcttatc gcgatctgcc
    10861 gctgcgtatg gccgagtttg gtagctgcca ccgtaacgag ccgtcaggtt cgctgcatgg
    10921 cctgatgcgc gtgcgtggat ttacccagga tgacgcgcat atcttctgta ctgaagaaca
    10981 aattcgcgat gaagttaacg gatgtatccg tttagtctat gatatgtaca gcacttttgg
    11041 cttcgagaag atcgtcgtca aactctccac tcgtcctgaa aaacgtattg gcagcgacga
    11101 aatgtgggat cgtgctgagg cggacctggc ggttgcgctg gaagaaaaca acatcccgtt
    11161 tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa attgaattta ccctgtatga
    11221 ctgcctcgat cgtgcatggc agtgcggtac agtacagctg gacttctcct tgccgtctcg
    11281 tctgagcgct tcttatgtag gcgaagacaa tgaacgtaaa gtaccggtaa tgattcaccg
    11341 cgcaattctg gggtcgatgg aacgtttcat cggtatcctg accgaagagt tcgctggttt
    11401 cttcccgacc tggcttgcgc cggttcaggt tgttatcatg aatattaccg attcacagtc
    11461 tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg ggcattcgtg ttaaagcaga
    11521 cttgagaaat gagaagattg gctttaaaat ccgcgagcac actttgcgtc gcgtcccata
    11581 tatgctggtc tgtggtgata aagaggtgga atcaggcaaa gttgccgttc gcacccgccg
    11641 tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc gagaagctgc aacaagagat
    11701 tcgcagccgc agtcttaaac aattggagga ataaggtatt aaaggcggaa aacgagttca
    11761 aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc caggaagttc gcttaacagg
    11821 tctggaaggc gagcagcttg gtattgtgag tctgagagaa gctctggaga aagcagaaga
    11881 agccggagta gacttagtcg agatcagccc taacgccgag ccgccggttt gtcgtataat
    11941 ggattacggc aaattcctct atgaaaagag caagtcttct aaggaacaga agaaaaagca
    12001 aaaagttatc caggttaagg aaattaaatt ccgtcctggt acagatgaag gcgactatca
    12061 ggtaaaactc cgcagcctga ttcgctttct cgaagagggt gataaagcca aaatcacgct
    12121 gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt atggaagtgc ttaaccgcgt
    12181 gaaagacgat ttgcaagaac tggcagtggt cgaatccttc ccaacgaaga tcgaaggccg
    12241 ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa ggccttcaag tagcactgtc
    12301 tgtggagcct tcgggcttca cagactttgt tcgcctttgt ttcgtttatt aacaatgcga
    12361 agtggaagtt attaaaatgc caaaaattaa gaccgtacgc ggtgctgcta agcgcttcaa
    12421 aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac ctgcgtcaca ttctgaccaa
    12481 aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc atggtttcca aaggcgatct
    12541 gggcctggta atcgcgtgcc tgccgtacgc ataagccgtt aacgttttta actttttaat
    12601 tagaatatag atacaggaga gcacatatgg ctcgcgtaaa acgtggtgtt attgcacgcg
    12661 cacgtcacaa gaaaattttg aaacaagcta aaggctacta cggtgcgcgt tctcgcgtat
    12721 accgcgttgc cttccaggct gttatcaaag ctggtcagta tgcttaccgt gaccgtcgtc
    12781 aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat caacgcagca gcacgtcaga
    12841 acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa agcctctgtt gaaatcgacc
    12901 gtaagatcct ggctgatatc gcagtattcg acaaagtagc gttcaccgct ctggttgaaa
    12961 aagcgaaagc agctctggca taagccagtt gaaagaggga gctagtctcc ctcttttcgt
    13021 ttcaacgcca tcaaaacatt gacttttatc gccgtagcct tttcaataaa ggtcttttga
    13081 agagtaccaa aaggtaacgc aagcaatgaa tgctgctatt ttccgcttct ttttttactt
    13141 tagcacctga atccaggagg ctagcgcgtg agaagagaaa cggaaaacag cgcctgaaag
    13201 cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa ataaaaagcc agtctatcag
    13261 gcgtaattgt cgcagccagt ctggatacgg ccagtgcgga gaaaccggag cgtacttaag
    13321 tacgtgagga tttcgagcac tgcccgggac caaaatggca agtaaaatag cctgatggga
    13381 taggctctaa gtccaacgaa ccagtgtcac cactgacaca atgaggaaaa ccatgtcaca
    13441 tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc caggcgtcag atgttgccgc
    13501 gttagacaat gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc ttcagatgac
    13561 gaccctgcgt gagctgccgc cagaagagcg tccggcagct ggtgcggtta tcaacgaagc
    13621 gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa gcgctgcact
    13681 gaatgcgcgt ctggcggcgg aaacgattga tgtctctctg ccaggtcgtc gcattgaaaa
    13741 cggcggtctg catccggtta cccgtaccat cgaccgtatc gaaagtttct tcggtgagct
    13801 tggctttacc gtggcaaccg ggccggaaat cgaagacgat tatcataact tcgatgctct
    13861 gaacattcct ggtcaccacc cggcgcgcgc tgaccacgac actttctggt ttgacgctac
    13921 ccgcctgctg cgtacccaga cctctggcgt acagatccgc accatgaaag cccagcagcc
    13981 accgattcgt atcatcgcgc ctggccgtgt ttatcgtaac gactacgacc agactcacac
    14041 gccgatgttc catcagatgg aaggtctgat tgttgatacc aacatcagct ttaccaacct
    14101 gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag gaagatttgc agattcgctt
    14161 ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa gtggatgtca tgggtaaaaa
    14221 cggtaaatgg ctggaagtac tgggctgcgg gatggtgcat ccgaacgtgc tgcgtaacgt
    14281 tggcatcgac ccggaagttt actctggttt cgccttcggg atggggatgg agcgtctgac
    14341 tatgttgcgt tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct
    14401 caaacagttt aaataaggca ggaatagatt atgaaattca gtgaactgtg gttacgcgaa
    14461 tgggtgaacc cggcgattga tagcgatgcg ctggcgaatc aaatcactat ggcgggcctg
    14521 gaagttgacg gtgtagaacc ggttgccggt agcttccacg gcgtggtcgt tggtgaagtg
    14581 gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc
    14641 ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgtg
    14701 gcggtagcga ccattggtgc tgttctgccg ggtgatttca aaattaaagc ggcgaaactt
    14761 cgtggcgaac cgtctgaagg gatgctgtgc tccttctctg agctgggaat ttctgacgat
    14821 cataacggca taatcgagct gcctgcggat gcgccgattg gcaccgacat ccgcgaatac
    14881 ctgaaactcg atgacaacac catcgaaatc agcgtaacgc caaaccgtgc cgactgttta
    14941 ggtatcattg gtgttgcgcg tgacgttgcc gtgctgaatc agctgccgct ggttgaaccg
    15001 gaaatcgttc cggttggtgc gaccatcgac gacacgctgc cgattacagt cgaagcgccg
    15061 gaagcctgcc cgcgttatct tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact
    15121 ccgctgtgga tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt
    15181 gacgtcacca actatgtgct gctcgaactg ggccagccga tgcacgcttt cgataaagat
    15241 cgcattgaag gcggcattgt ggtgcggatg gcgaaagagg gtgaaacgct ggtgctgctc
    15301 gacggcactg aagcgaagct gaatgctgac accctggtta tcgccgacca caacaaggcg
    15361 ctggcgatgg gcggcatttt tggtggcgaa cactctggcg tgaatgacga aacgcaaaac
    15421 gtcctgctgg aatgcgcttt cttcagcccg ctgtctatca ccggtcgtgc tcgtcgtcat
    15481 ggcctgcata ctgatgcgtc tcaccgttat gagcgtggcg ttgatccggc actgcagtac
    15541 aaagcgatgg aacgtgcgac ccgtctgctg atcgacatct gcggtggtga ggctggtccg
    15601 gtaattgata tcaccagcga agcaacgctg ccgaagcgtg caaccatcac tttgcgtcgt
    15661 agcaaactgg atcgcctgat cggccatcat attgcggatg agcaggtaac tgacattctg
    15721 cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgagt ggcaggcagt tgcgccgagc
    15781 tggcgtttcg atatggagat tgaagaagat ctggtcgaag aagtcgcgcg tgtttacggc
    15841 tacaacaaca tcccggatga gccggtacag gcaagcctga ttatgggtac tcaccgtgaa
    15901 gctgacctgt cgctcaagcg cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg
    15961 atcacctaca gcttcgttga tccgaaagtg cagcagatga tccatccagg cgttgaagcc
    16021 ttactgctgc caagcccgat ctctgttgaa atgtcagcaa tgcgtctttc tctgtggacc
    16081 ggcctgctgg caaccgtggt gtacaaccag aaccgtcagc agaaccgtgt gcgcattttc
    16141 gaaagcggtc tgcgtttcgt accagatact caggcaccgt tgggcattcg tcaggatctg
    16201 atgttagccg gtgtgatttg cggtaaccgt tacgaagagc actggaacct ggcaaaagag
    16261 accgttgatt tctatgattt gaaaggcgat cttgaatccg ttctcgacct gaccggtaaa
    16321 ctgaatgagg ttgagttccg tgcagaagcg aatccggcac tgcatccggg gcaatccgca
    16381 gcgatttatc tgaaaggtga acgtattggt tttgttgggg ttgttcatcc tgaactggaa
    16441 cgtaaactgg atcttaacgg tcgcactctg gtgttcgaac tggagtggaa caagctcgca
    16501 gaccgcgtgg tgcctcaggc gcgcgagatt tctcgcttcc cggcgaaccg tcgtgacatc
    16561 gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt tatccgaatg taagaaagtt
    16621 ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt accgcggtaa gggtgttgcg
    16681 gaggggtata agagcctcgc cataagcctg atcctgcaag ataccagccg tacactcgaa
    16741 gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag
    16801 gcatcattga gggattgaac ctatggcgct tacaaaagct gaaatgtcag aatatctgtt
    16861 tgataagctt gggcttagca agcgggatgc caaagaactg gttgaactgt ttttcgaaga
    16921 gatccgtcgc gctctggaaa acggcgaaca ggtgaaactc tctggttttg gtaacttcga
    16981 tctgcgtgat aagaatcaac gcccgggacg taacccgaaa acgggcgagg atattcccat
    17041 tacagcacgg cgcgtggtga ccttcagacc cgggcagaag ttaaaaagcc gggtcgaaaa
    17101 cgcttcgccc aaagacgagt aatctgatct aactaaaaag gccgcgttgc ggcctttttt
    17161 cttttcactg tcgaagagtc accgtaaaat caacgccatg acacttcagc agaacggata
    17221 ccatgctgac acttgcccgc caacaacagc gacaaaatat tcgctggtta ttatgcctgt
    17281 cagttttgat gctgctggcg cttctcttaa gcctttgcgc cggtgaacaa tggatctcgc
    17341 caggtgactg gttttctcct cgtggcgaac tgttcgtctg gcaaattcgc ctgccacgta
    17401 cgctggctgt attgctggtt ggtgcggcgc tggctatatc cggcgctgta atgcaggcgt
    17461 tgtttgaaaa tcctctggca gaacctggac tacttggcgt ctctaacggc gcaggcgtgg
    17521 ggcttatcgc cgcggtattg cttgggcaag ggcaactccc caactgggcg ctagggctgt
    17581 gtgcgattgc tggcgcgctt atcatcactt taatactctt acgtttcgcc cgtcgtcatc
    17641 tttcgaccag tcggttattg ctggctggcg ttgcattagg gattatctgt agcgcactaa
    17701 tgacgtgggc tatctacttt tccacctctg ttgatttacg tcagctgatg tactggatga
    17761 tgggcggttt tggcggcgta gactggcggc aaagctggct gatgctggca ttgatccccg
    17821 tgttgttgtg gatctgttgt cagtccaggc cgatgaatat gttagcactt ggcgagatct
    17881 cggcgcggca actgggttta cccctgtggt tctggcgcaa tgtgctggtg gcagcgaccg
    17941 gctggatggt tggcgtcagt gtagcgctgg cgggtgctat cggctttatt ggtctggtga
    18001 tcccacatat tcttcggttg tgtggtttaa ccgatcatcg cgtattactt cccggctgcg
    18061 cgctggcagg ggcgagcgca ttgctgctgg ccgatattgt agcgcgcctg gcattagctg
    18121 ccgcagagct gcctattggc gtggtcaccg caacgttggg tgcgccggtg tttatctggt
    18181 tattgttaaa agcaggacgt tagccgcaaa aagacggtct atgattaaaa gctaagattt
    18241 ttactgacca cacccaggag aaacgatgca agattccatt ctgacgaccg tagtgaaaga
    18301 tatcgacggt gaagtgacca cgctggagaa gttcgccggt aatgtgctgt tgattgtcaa
    18361 tgtcgcctca aagtgtggct taacgccgca atatgagcag ttggagaata ttcagaaagc
    18421 ctgggccgat cgaggttttg tggtgctggg attcccgtgc aaccagtttc tggaacaaga
    18481 accgggcagc gatgaagaga ttaaaactta ctgtaccacc acatgggggg tgacgttccc
    18541 gatgttcagt aagattgaag ttaacggcga aggacgccat ccgctgtatc aaaaattgat
    18601 tgccgcagcg ccgaccgcag tcgcgccgga agagagcgga ttctatgccc gtatggtcag
    18661 caaaggtcgt gcaccgctgt accctgatga tattttatgg aattttgaga aatttttagt
    18721 tggcagggac ggattggtca tccagcgttt ttccccggat atgacgccgg aagatcccat
    18781 tgtgatggaa agcattaaac tggcgttggc aaaataatgt ctattgtgat gcagttacag
    18841 gatgttgcgg aatctacccg cctggggccg ctttctggcg agattcgggc tggggagatc
    18901 ctgcacctgg tggggccgaa tggcgcgggt aagagtacct tactggcgcg aatggccgga
    18961 atgaccagcg gtaagggaag cattcagttc gcggggcaac cactggaagc atggtccgca
    19021 acaaaactcg cgctgcatcg cgcctatctt tcacaacagc agacgccgcc gtttgcaatg
    19081 ccggtctggc actacctgac actgcatcag cacgataaaa cgcgtaccga actactgaat
    19141 gatgtcgcag gggcgctggc tcttgatgac aaactcggtc gtagcaccaa tcaactttcc
    19201 ggcggtgaat ggcaacgcgt acgtcttgct gcggtggtgt tgcaaatcac accacaagcc
    19261 aatcccgcag gccaattgct gcttcttgat gagccgatga acagtcttga tgttgcgcaa
    19321 caaagtgcgt tagacaaaat tctgagcgct ctgtgtcagc aaggactggc gattgtgatg
    19381 agcagtcacg atctcaacca cacgttgcgt catgcgcatc gggcgtggtt gctaaaaggt
    19441 ggaaaaatgc tggccagtgg acgcagagaa gaggtgctca cgccgccaaa tctggcgcag
    19501 gcctatggga tgaattttcg ccgtctggat atcgaaggtc acagaatgct gatttcgacc
    19561 atctgatttc attagtatct tgaaaatcgg gtaattacga cgctaaatta atacaagaat
    19621 aaaaaaacag aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt
    19681 ctggccgggt gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt
    19741 accgttattg ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat
    19801 ggtggcatga cgcggcgcgg tgtggactgt tcgggatttg tggtggtgac gatgcgcgat
    19861 cgttttgatt tgcagctgcc ccgagaaacc aaagaacaag cctctatcgg cacgcaaatt
    19921 gataaagacg agttgctgcc tggtgacctg gtctttttca aaacgggttc cggacaaaat
    19981 ggtttgcatg taggtattta tgataccaac aaccaattta tccacgcctc taccagcaag
    20041 ggagtgatgc gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga
    20101 cgaatctagt atgtcgaaaa ttggacggcg atgatacatc gccgtccatt tactccagaa
    20161 tacagtagta ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata
    20221 atctaaaaaa aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactccttc
    20281 tgtaattgtt atgtcttatg gcctcatccc ttttagccgg atactgaaaa acatccttcg
    20341 agagggacgg ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc
    20401 ctgccgataa gagataacca gcaagacctt gtcggcgttg agttaattac ccatttctcc
    20461 agtgaagatg gtacggttcg tatcccgacg agtcgggtta tagcgcaact taccgaggag
    20521 caacattggc aattattttc agagcagttg gaattactaa aatcatgcca acattttttt
    20581 attcaacata aactctttgc ctggcttaat ttaacgccac aagtggctag attattatta
    20641 gagagggata attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat
    20701 gagaattacc cgcatctcaa tgaaggaaaa gataatcgcg gtcttttatc cttatcgcaa
    20761 gtgtatcccc ttgtgttagg taatttggga gcaggcaata gcacaatgaa agcggttttt
    20821 gatggtcttt tcacccgcgt catgctggat aaaagtttta ttcagcaaca aatcacgcac
    20881 cgctccttcg aaccgtttat tcgtgcgatc aaggcgcaaa tttccccttg ttgcaattgc
    20941 atcatcgcgg gaggcattga tacggcagaa atattggcac agattacccc cttcgatttc
    21001 catgctctcc agggatgcct gtggcctgcc gttcccataa atcagataac gacgttggtt
    21061 cagcgataac cctcctgttt gctggtgttt aagacgagag taaccgtcta cactatcaaa
    21121 caggaggatc tatgaccctg tcttttatta cccgctggcg cgatgaattg ccagcaacct
    21181 atacagcact ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac
    21241 tcgctaacac gctgagtatt ccatcgtcgc tgtttaaaaa tggcgcaggt gtctggggcg
    21301 gcgaaaccct actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg
    21361 gcgtctgggc gggccaactg ggtgatgggc gcggcatttt acttggcgaa caactgcttg
    21421 ctgatggcac tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa
    21481 tgggtgatgg acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga
    21541 tgcattatct gggcattccg acgacccgcg cgttaagtat cgtcaccagc aattcgccag
    21601 tgtatcggga aacggtggag ccaggcgcga tgctgatgcg tgtggcacca agtcatctgc
    21661 gctttggcca tttcgaacat ttttactatc gacgcgagcc ggaaaaggtt cgtcagttgg
    21721 ctgactttgc cattcgtcat tactggtcac atcttgaaga tgatgaggac aaataccgtc
    21781 tctggtttaa cgatgttgtc gcacgtaccg catcgttaat tgcccaatgg cagacggtcg
    21841 gctttgctca tggggtgatg aataccgaca acatgtcgct gctgggactg acgcttgatt
    21901 acgggccgtt tggttttctt aatgattacg aacccggttt tatttgtaat cactcggatc
    21961 atcaagggcg ttacagcttt gacaatcaac ctgctgtcgc gttgtggaat ttacagcgtc
    22021 tggcgcagac attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct
    22081 atcagcaggt tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatga
    22141 cggagcaaaa agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc
    22201 gcagcgatta tacccgcaca tttcgcatgc tgagtctgac cgagcagcac agcgcggcgt
    22261 caccgctacg tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc
    22321 gagggcgttt gcaacaagac gaggttagcg atagtgagcg tcagcagctg atgcaaagcg
    22381 ttaaccccgc tctggtattg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa
    22441 agggtgatat gacggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca
    22501 gagatgatga ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct
    22561 cgagttaaag gctacttggt caacagcagc ttgccagcct gagttttacg gagcagatat
    22621 tcttgcccgt catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggaa
    22681 attcgccgaa taggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt
    22741 tttctgctat ccgtataacg cataaaaatt aataataatt gatataacaa tgattcttat
    22801 tatcaatatt atggctgacc agagcaagca gtaatgtgtt gagaagacaa caagttgcgc
    22861 agaaatgccc atgccgtcag gaatgggcac gcattcagaa gcgggtatct accgcagagg
    22921 cgagtttttc gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt
    22981 gaccgtaagt gagcggctga ctgccgacga ttttttgcgt tccttcgcgc aggaaacttt
    23041 ccgccataat tccagcaatc gccgtagagc cattgcggat ttgctgacaa atatcctcac
    23101 aaacttctaa ctggcgacgg tgctgcttct ggcagttacc gtggctgaaa tcgaccacca
    23161 gatgttcagg taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat
    23221 gataattcgg ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct
    23281 gatagatggt catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg
    23341 cgcggatagc atccacagca atccgcgtgt tgccatcggt accattttta aaacctaccg
    23401 gacaggagag tgccgaagcc atttcacggt ggatctgact ttcggtagta cgtgcgccaa
    23461 tcgcgcccca actgattaaa tcagcaataa actgaccggt caccatatcg aggaactcag
    23521 tcgcggtagg gacacccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac
    23581 cgtgatttac ccgatagctg ccgtttaaat ctggatcgga gattagtcct ttccagccga
    23641 caacagttcg tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact
    23701 ggttgcgcag cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg
    23761 agcaggggcc aataatgacc aacagtcgct tatcttcacc attgagtatt ttttcaattc
    23821 tgcggcggga gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga
    23881 gttcggcggg cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca
    23941 ttacaagtct cagaaatttg ttgcttctac ggcaggactg ccgggaagtg atgcgcatca
    24001 cgataaacca atcgctgctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta
    24061 gcatgataac aaaaaaataa gctaatgcac tagttctcta gtacattcgg cgactaaggc
    24121 ccatgatatc gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc
    24181 acgggatctg atttttacgg tacaaggctt ccacttccgc gacttccatc ctgcactgac
    24241 gaagcgaggc atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg
    24301 ggtcgatagt caggccgaac aatttatgct gaagcggttt gagcgacgcg ggtagcacca
    24361 gattatccat atcgtcggca ataaaggggt agtttgccgc gcggatacca aattgcattg
    24421 ccagatacag actggtgggg gttttaccac agcgagaaac accgagcagg atcacctgag
    24481 cctgatccag attgcgcaac gaaatgccgt catcgtgggc gagggtgtaa tcaatcgccg
    24541 caatgcgcgc atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa
    24601 tcggcgttgg atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat
    24661 cctggcaaaa gccttcactt tgcaagatga tggcgcgaat ctccggcaac acgatggagt
    24721 agaagaccag cgggcgcacg cctgtctggt gataaattgc gtcaatctga tccttcactg
    24781 cccgtgcacg gctctcattt tcgacaaacg gcagcgtgat gctgctgata gtgacgggaa
    24841 attgtgacat tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat
    24901 aaaaaacgtg gcgatcaaca gcattatcca ttttgttctt cccgtgatgc agacataatt
    24961 ctatgaaagc ataaattaaa aacgcacaga agcgtagaac gttatgtctg gtttataaaa
    25021 tgaa
//
LOCUS       DAZGCQ010000073        24859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000073 DAZGCQ010000000
VERSION     DAZGCQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.073"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1803)
                     /locus_tag="ACHK07_003281"
     CDS             complement(<1..1803)
                     /locus_tag="ACHK07_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO8103571.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVVMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGY"
     gene            complement(2645..3736)
                     /gene="ychF"
                     /locus_tag="ACHK07_003282"
     CDS             complement(2645..3736)
                     /gene="ychF"
                     /locus_tag="ACHK07_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO8103572.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(3853..4437)
                     /gene="pth"
                     /locus_tag="ACHK07_003283"
     CDS             complement(3853..4437)
                     /gene="pth"
                     /locus_tag="ACHK07_003283"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO8103573.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            4715..4993
                     /gene="ychH"
                     /locus_tag="ACHK07_003284"
     CDS             4715..4993
                     /gene="ychH"
                     /locus_tag="ACHK07_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO8103574.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(5048..6727)
                     /gene="dauA"
                     /locus_tag="ACHK07_003285"
     CDS             complement(5048..6727)
                     /gene="dauA"
                     /locus_tag="ACHK07_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO8103575.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(6852..7799)
                     /gene="prs"
                     /locus_tag="ACHK07_003286"
     CDS             complement(6852..7799)
                     /gene="prs"
                     /locus_tag="ACHK07_003286"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO8103576.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(7950..8801)
                     /gene="ispE"
                     /locus_tag="ACHK07_003287"
     CDS             complement(7950..8801)
                     /gene="ispE"
                     /locus_tag="ACHK07_003287"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO8103577.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVAPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(8801..9424)
                     /gene="lolB"
                     /locus_tag="ACHK07_003288"
     CDS             complement(8801..9424)
                     /gene="lolB"
                     /locus_tag="ACHK07_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO8103578.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            9638..10894
                     /gene="hemA"
                     /locus_tag="ACHK07_003289"
     CDS             9638..10894
                     /gene="hemA"
                     /locus_tag="ACHK07_003289"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO8103579.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            10936..12018
                     /gene="prfA"
                     /locus_tag="ACHK07_003290"
     CDS             10936..12018
                     /gene="prfA"
                     /locus_tag="ACHK07_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO8103580.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            12018..12851
                     /gene="prmC"
                     /locus_tag="ACHK07_003291"
     CDS             12018..12851
                     /gene="prmC"
                     /locus_tag="ACHK07_003291"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8103581.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            12848..13240
                     /gene="sirB2"
                     /locus_tag="ACHK07_003292"
     CDS             12848..13240
                     /gene="sirB2"
                     /locus_tag="ACHK07_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO8103582.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            13244..14053
                     /gene="sirB1"
                     /locus_tag="ACHK07_003293"
     CDS             13244..14053
                     /gene="sirB1"
                     /locus_tag="ACHK07_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO8103583.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            14089..14943
                     /gene="kdsA"
                     /locus_tag="ACHK07_003294"
     CDS             14089..14943
                     /gene="kdsA"
                     /locus_tag="ACHK07_003294"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO8103584.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(15092..15199)
                     /locus_tag="ACHK07_003295"
     CDS             complement(15092..15199)
                     /locus_tag="ACHK07_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8103585.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(15628..15735)
                     /locus_tag="ACHK07_003296"
     CDS             complement(15628..15735)
                     /locus_tag="ACHK07_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8103586.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(16140..17240)
                     /gene="chaA"
                     /locus_tag="ACHK07_003297"
     CDS             complement(16140..17240)
                     /gene="chaA"
                     /locus_tag="ACHK07_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO8103587.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            17510..17740
                     /gene="chaB"
                     /locus_tag="ACHK07_003298"
     CDS             17510..17740
                     /gene="chaB"
                     /locus_tag="ACHK07_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO8103588.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            17898..18593
                     /gene="chaC"
                     /locus_tag="ACHK07_003299"
     CDS             17898..18593
                     /gene="chaC"
                     /locus_tag="ACHK07_003299"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO8103589.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTALQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(18637..18990)
                     /gene="ychN"
                     /locus_tag="ACHK07_003300"
     CDS             complement(18637..18990)
                     /gene="ychN"
                     /locus_tag="ACHK07_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO8103590.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            19176..20570
                     /gene="ychO"
                     /locus_tag="ACHK07_003301"
                     /gene_synonym="iatA"
     CDS             19176..20570
                     /gene="ychO"
                     /locus_tag="ACHK07_003301"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO8103591.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(20571..21221)
                     /gene="narL"
                     /locus_tag="ACHK07_003302"
     CDS             complement(20571..21221)
                     /gene="narL"
                     /locus_tag="ACHK07_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO8103592.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(21214..23010)
                     /gene="narX"
                     /locus_tag="ACHK07_003303"
     CDS             complement(21214..23010)
                     /gene="narX"
                     /locus_tag="ACHK07_003303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO8103593.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            23349..24740
                     /gene="narK"
                     /locus_tag="ACHK07_003304"
     CDS             23349..24740
                     /gene="narK"
                     /locus_tag="ACHK07_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO8103594.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         6119 a         6189 c         6338 g         6213 t
ORIGIN      
        1 atatcccccc aactcaacct gagaagacgc gctgaacgag gccgcaccgt cagtggtttt
       61 taccactgtc agaacttcat ttcccgttgt ggcctcgctg ccctggttgc ggatagccaa
      121 tacatgatta ccagcactac tcccgctgat attcaataaa tcccctttat tattaacgcc
      181 attaccctcg ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc
      241 tacgtttaat gtggtaaatg tgccggcagt tgacccgtgg ctggcaaaat cgacagttga
      301 atggctcagc gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt
      361 cattacaacg tccagtttcc cgccattgac attatcgctg agggaggaac ctgtccaaac
      421 cgacccaggg tgcatatcca gattgattag cccacctttg gaaagaacgc taccgttgat
      481 aagcatacga cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc
      541 gatggccatc tggtcgggtg tactcatatc aatgaccaga tcgctcgtga ggtcgatctg
      601 gctgttggtc atggcataaa tccccctggc tcctgccgcg ccggtgatag ccaaactgtc
      661 tccggttatt ctaccgccgc tgagcgccca caaacccagc gccagactgc cattacgatc
      721 aaccgtaata tccgtatttt gcatgttgat aacagccgtt gccgtctggg ccgaggcacc
      781 ataagagccg ccggaaaaga tgctgtttcg ttgcgctgcc gtgccagaaa aattgattgt
      841 cgcgtctgaa ccactggcta cgagaccacc gccctgcgcg gaagaaatat ggctatctgc
      901 accgatagtg gttgtaccac cacgaacttc gacgccattg gccgcggctc cagttacatc
      961 aacagtgagt gcattcgcgc taacctggcc aaagctccag aggccgtgag cattatcgcc
     1021 attggtttta atggtactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat
     1081 ccccatggcg ctgtagccct gaacatcgat tgtcaggtct gtcgccgtaa aacgcgcagc
     1141 accattggcg ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtctt
     1201 gattttactt ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat
     1261 accgttcgag ttttcaatgg tgaattgagt ggctgttagt gttgagctat gcccaataat
     1321 gatgccgtca tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc
     1381 accaattaaa ttaatgccga tggcagaggt ttgaccaaca acatctactg tgagtcggtt
     1441 agctgtcagg gagctgtttt tgccctgaat aatgattcct tttgcatatc cagaggcgtc
     1501 gttgacattg acggtgacat cattacccag gttgatattg ccaggcgtgt ttcctgctgg
     1561 ggtcattaca ccatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc
     1621 atcattaata gtaatattgg tgccatattg acttcctgta tcagcagcta tcagcagggg
     1681 ggaaaagata attgcattca aaccaatagc gccaaatttt atcagttgaa ttgagggcga
     1741 acggattttt aattcagcga gacgatcggc tttggtattc ccattgtgtt gtttgatgcc
     1801 cataccactc ctatatagta cccaggctat gggtctatgg attttgtcat tttgtttaag
     1861 tcaatgaata atatcctctt atcatcatga tatttgtatg gtgcaatcat gatgagctgc
     1921 tatgagaata attctctatt accgggcaaa atcagaaaca caggaattaa tatatattta
     1981 acatccccct tacaaaggtg agtttagatc gagttattcc ctaagttgtc gttacttatt
     2041 ccaattcgtt actatgaata tgacaaaaac attacaccaa acagcggtta aataatttgg
     2101 taatattttt ggtaataatc ctaattagtt atgggatttt cgctcgatgt atctgcataa
     2161 caaaaacaat atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt
     2221 catttttgat caatgatttt tctggcatat cgttattatc gtgctattgg ttatcatttt
     2281 catcttgcca ggatcgcacg taatctcatt attcggggac tttatctgcg ggatattacc
     2341 cttcaggtaa gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg
     2401 aagggggtga tgtgtagtgg aacaaaaact tacgttatat cactactaat gagatatccg
     2461 tcatctgaac actgatgtct ttaattattc tggtggttca ctgtaggcaa taagcacaaa
     2521 agtgtaggat gttacaagaa tgattaggac tcggtgaaat gaaaaatcca cgcaattgcg
     2581 tggattttat atacttttgc gctcttcatg agatttagcg aaacctcatg agacaacaaa
     2641 ttaattagac gttgaaaagg aagttcatca catcgccatc tttaacgatg taatctttac
     2701 cttctgcacg cattttgcct gcttctttcg cgccttgttc acctttgtaa gtgatgaaat
     2761 cttcaaacga gatggtttgt gcacggataa agcctttttc aaaatcagta tggattttgc
     2821 ccgctgcctg cggcgcggtt gctccaactg gaatggtcca tgcacgcact tctttcaccc
     2881 cagcggtgaa gtaagtttgc aggttcagca gcttataacc ggcacggatc acacggttca
     2941 ggcccggctc ttccagccca agctcctgca taaactcgtc acgttcttcg tcgtccagtt
     3001 cggcaatgtc tgcttcaaca gcggcacaaa ccggaaccac aacagaacct tctttcgccg
     3061 cgatttcacg cacctggtca agatatgggt tgttttcaaa accgtcttcg ttgacgttgg
     3121 cgatgtacat tgttggtttt agcgtcagga agctcaggta acgaatagcc gctttctctt
     3181 cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc cagctggggc aggcattttt
     3241 ccaggaccgc cagctcagct ttcgcgtctt tatcgccacc tttggctttc ttctgtacgc
     3301 gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc cagttcggtg ttgataactt
     3361 caatatcgtc agccgggtta actttgccgg aaacgtgaat gatgttgtca ttttcaaagc
     3421 agcgaacaac gtgaccgatc gcttcggttt cacggatgtt ggtcaggaac tggttaccca
     3481 gaccttcgcc tttcgatgcg ccttttacca gaccggcgat atcgacaaat tccatggtcg
     3541 tgggaagcgt acgttgcggt tttacgattt cagccagttg atccaggcga ggatcaggca
     3601 ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg aaagttggcc gcttcaatac
     3661 cggctttggt cagcgcgttg aacagggtag atttcccgac gttgggcaaa ccgacgatac
     3721 cgcatttgaa tcccatgatt taaatcacct taatatctta ataatcaacc tgttattgct
     3781 aacagattgc agaaatggaa ataactttgc ctattataca cggcactcga caaaaatgcc
     3841 gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg ttcgttgctt tggtcaagcc
     3901 atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg atggcttcat caattaactt
     3961 ctgttcacta acaggcggtt tgcctaacac aaaaccgaca actttatttt tatcgcccgg
     4021 atgaccgatt ccgatgcgta aacggtgaaa gttagggtta ttacccaatt tactgatgat
     4081 gtctttcagt ccattgtgac caccatggcc accgcccaac ttaaatttgg cgacgccagg
     4141 aggcagatcc agttcgtcgt gggccaccag aatttcgtcc ggattaatgc ggaaaaaact
     4201 ggccatcgcc gcaacggctt tgccgctgag attcataaat gtagtcggga ctaacaggcg
     4261 gacatcttcg cctccaaggg tgactcgcga agtataacca aagaatttag cctcttcgcg
     4321 cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac caggcaccag cattatgtcg
     4381 cgttgcggcg tattcagcac cggggttcgc caggccgaca atcaatttaa tcgtcacgtt
     4441 tttttgtcct gagtgtgtac ataactggcg cgtagtttac tggttgcggc cccgcttgac
     4501 aaaaaactgc gtatcaaatg cagataacgt aataattgcc tgagtggact attagaaagt
     4561 cagggtgttc aggcgtttat ttgtaaagtt ttgttgaaat aagggttgta attgtgatca
     4621 cgcccgcaca taacccacag ggtgttgtct atactttaca cataaggaag aggggtattc
     4681 cctgttacaa cccagaaagt tccggaggtg acatatgaaa cgcaaaaacg cttcgttact
     4741 cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg gtcggcgtgg ggtattcaat
     4801 cctcaaccag ttaccacagt ttaatatgcc ccagtatttc gcacatggtg cagtgctaag
     4861 tattttcgtc ggtgccattc tctggctggc gggtgcccgt gttggcgggc atgaacaggt
     4921 gtgcgaccgt tactggtggg ttcgccacta tgacaaacgt tgccgccgta gcgataatcg
     4981 ccgtcatagc taacaaatac tgcagtttgc tgaccagtca gtttgcgctg actggtcaat
     5041 ctcgaactta taaatccgcc atcgccgctc gacgattcgg gaagaacgcg aggcgtcccg
     5101 ggatcggttg aatgcctgcg cgagccatag tgcgcagtgg ctggaattcc acgttgcaca
     5161 cgcgcagttc acatccttcg ggcagacgct tcacaaaacg ctggaacgca tcaagaccac
     5221 cagcatcaag taccggaacg gcatcccact tcagaatcac aatccgtttg ccttcaagac
     5281 gtgactccag gtccgtgaat aagccttcag cagcagcaaa aaacagcggg ccaataacgc
     5341 gcagaaccag gacatcgtct ggaacatcta cgactaccgg tgccaggcga gtcatacgtg
     5401 cgatacgacg cataaacagc agcgatgcca gcacgatccc cacgctgatg gcaataacca
     5461 tatcaaacag cacggtcagc gacatgcaca gcagcatgac aatgatgtca tctttcggtg
     5521 catgacgcag caagtcgacc actttatgcg cttcactcat gttccacgcc accatcaaca
     5581 gcagggctgc catagcggaa agcggcagcc aggagagcag cggtgccagt accagcaggg
     5641 caagaataac cagaatagag tggatcaccg ccgagatagg ggaagttgcc ccggcacgga
     5701 cgttagcggc agaacgcgcg atggcagctg tagcggtaat accaccaaag aacggagcga
     5761 tgatattccc cagtccctgt ccaaccagtt cgctattcgc cttgtgtttc gtcccggtca
     5821 taccatccag caccacggcg cagagcagag attcgattgc gccgagcatt gccattgaga
     5881 atgccgcagg cagcagtgtg cgaatagaat cccaggttaa cgtgaattct gaattaggca
     5941 gatcccacgg cagcaccagt tgcggcagca gttgcggaat accgttaccc tgagaaccat
     6001 cggccaggac gtagtggaat tgcgaaccga tggtagcaac atgtccgccg agcaggttaa
     6061 caatccccat caccgcgcaa ccagccagca aggccggaag gtgaccgggt aaacgaatgc
     6121 ccagacgcgg ccagaaaaca agaataccta gcgtcacaat gccaatggca gcatcaccca
     6181 cattaatggt cggcagcgcc ataaataatg cgccgacttt ttgtagataa tgttccggga
     6241 catgggccat ttgcagaccg agaaaatctt taatctgcat ggtaccgatg gtgatcccga
     6301 tacccgaggt gaaacctaag gtgacggaaa ccggaatata ctcaatcagg cgaccaaagc
     6361 gtgccagacc catcagaatc aaaaagatcc ccgacagcaa ggtcgcaacc agcagtcctg
     6421 ccagcccaaa ctgttgcgaa acgggataga gaattaccac aaatgccgca gtcggaccgg
     6481 aaacgctaaa gcgtgaccca cccgtcagag caatgacaat ccccgcaaca gctgcggtat
     6541 ataaaccgta ctggggtgcc acaccactac caatagccaa cgccatcgcc agcgggatag
     6601 caataatccc gacggttatc ccggcaatca ggtcacgggt aaaccgtgcg gcagtatatt
     6661 tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat cacatgtgag gaaaatattt
     6721 tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc aactaaatgg caggtgaagg
     6781 aggcataggt catacaaatg gatattacag acaaaaaaac ccgccgcagc gggtctttga
     6841 gccgggttcg attagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga
     6901 atcgcttcgg ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt
     6961 tcatcgctca gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg
     7021 ttgttcgccg cgttgccaga gaagatcggg tgagtcgcat acgcaaatac acgtttagca
     7081 ccacgttctt tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca
     7141 tcgaccagta cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg
     7201 ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg
     7261 atagcgcggg cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga
     7321 ttcagctgca gcatgtcttc cagcaggatc gggctaccaa atacgttatc gaccggaacg
     7381 tcgaagaaac cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg
     7441 acgctggaga gaaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg
     7501 cgacggtcct ggcgcgcata gccaaagtag gggataacag cggtgatacg acctgcggaa
     7561 gcacgacgca gggcatcaac cataacgact aattccatca ggttgtcgtt agtaggggca
     7621 caagtggact ggatgatgaa aatatcacca ccgcgtacat tttcattaat ttgtacgctg
     7681 acttcgccat cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta caggcggttg
     7741 gcaatacgtt gtgctagttc cggggtggcg ttaccagcaa aaagcttcat atcaggcacg
     7801 agaagaacct caggcatgcg tccattggtg gaaagaatct gccgaaaact gtgcgggcca
     7861 ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga
     7921 cgttgtcacc gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga
     7981 ttagcgcctt tcgccacaaa gccattgagc cattccgggg cttgctctag cacctggcgg
     8041 gcttcagact ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt caggcgcgac
     8101 ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg
     8161 ataacctcgc aatcattgct gaattcacat tttagcaacg tttctattga cctttttggc
     8221 gtattgcgcg ggagttcagg atctttaaaa atcaccggag tcggaatact tacaccaggg
     8281 tgcgccacca gataccactt ctctggcgga gccaccggcg ttagtatttc accaacgcct
     8341 tcggcaaacg cggcatgccc ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc
     8401 atttccgcca gctcatccat gcttagcccg cattgccaga gatgatttaa tgccaccagg
     8461 accgtcgcgg cattggatga accaccgccg agaccgccgc ccatcggcaa acgcttgtca
     8521 atgctgatat tcgcaccgct tcccgtcgga agacgcccgc tgtctgccgc agttttcatc
     8581 aataaccgcg ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt
     8641 aacagacgaa tatccccatc gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga
     8701 aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa
     8761 aacagattaa gttttgccgg agagggccac tgtgtccgca ttatttcact atccagttat
     8821 ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc catattggct ggcatcgcag
     8881 gttgcgtttt ggtgtcataa ccaccataaa caaccttcca gtttttgcca ttctggctgt
     8941 aggtaatttc gctcaggcgg tactggtcgt ccagtttgta gtcggttgca tcacccggta
     9001 aacctaaaat ccactggcgc aagctgttga gcggaattgg cattccagtc aatttgccaa
     9061 tcatctcttc ggcgtcatcg gcggtataac gctgaccttt attgtcgact aactgcacgt
     9121 tacccggttg agcattcagc tccagttccg tgctgcccaa tgggttagtg agcagcagac
     9181 ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc gtacactttt tgttggtcag
     9241 aaatataagc gaacgcgccg cgagtctgat actgattaag attgcgcacg tcttgctgat
     9301 gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc tttgggcgtg gtaacggaac
     9361 aggcagtgag cacaagagca gccagcggta gcaggcggat aagacgaaaa tcgggcaggg
     9421 gcatagtgat gacaagtcct tgagatacgt tgcagttata acccttaatg ctagcgttac
     9481 cgtccgctat cgtctatgtt caagttgtct taattgccag aatctaacgg ctttcggcaa
     9541 ttactccaaa agggggcgct ctcttttatt gatcttacgc atcctgtatg atgcaagcag
     9601 actaacccta tcaacgttgg tattatttcc cgcagacatg acccttttag cactcggtat
     9661 caaccataaa acggcacctg tatcgctgcg agaacgtgta tcgttttcgc cggataagct
     9721 cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc
     9781 gacgtgcaac cgcacggaac tttatcttag cgttgaagag caggataacc tgcaagaggc
     9841 gttaatccgc tggctttgcg attatcacaa tcttaatgaa gaagatctgc gtaaaagcct
     9901 ctactggcat caggataacg acgcggttag ccatttaatg cgtgttgcca gcggcctgga
     9961 ttcactggtt cttggggagc cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc
    10021 gcaaaaaggc catatgaagg ccagcgaact ggaacgcatg ttccagaaat ctttctctgt
    10081 agcgaaacgc gttcgcactg aaacagatat cggtgccagc gctgtgtctg tcgcttttgc
    10141 ggcttgtacg ctggcgcggc agatctttga atcgctctct acggtcacag tgttgctggt
    10201 aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg cgcgaacata aagtacagaa
    10261 gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg aagtcggcgc
    10321 ggaagtgatt gccctgagtg atatcgacga acgtctgcgc gaagccgata tcatcatcag
    10381 ttccaccgcc agcccgttac cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag
    10441 ccgtcgcaac caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga
    10501 agttggcaaa ctggcgaatg cttatcttta tagcgttgat gatctgcaaa gcatcatttc
    10561 gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg gaaactattg tcgctcagga
    10621 aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta
    10681 tcgcagccag gcagagcaag ttcgcgatga gttaaccgcc aaagcgttag cggcccttga
    10741 gcagggcggc gacgcgcaag ccattatgca ggatctggca tggaaactga ctaaccgctt
    10801 gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt gacggggata acgaacgcct
    10861 gaatattctg cgcgacagcc tcgggctgga gtagcagtac atcattttct ttttttacag
    10921 ggtgcattta cgcctatgaa gccttctatc gttgccaaac tggaagccct gcatgaacgc
    10981 catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt
    11041 cgcgcattat cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt taccgactgg
    11101 caacaggttc aggaagatat cgaaaccgca cagatgatgc tcgacgatcc tgaaatgcgt
    11161 gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa
    11221 ttacaggttc tgttactgcc aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc
    11281 cgagccggaa ccggcggcga cgaagcggcg ctgttcgcag gcgatctgtt ccgtatgtac
    11341 agccgttacg ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa
    11401 catggtggtt ataaagagat catcgccaaa attagcggtg atggtgtgta tggtcgtctg
    11461 aaatttgaat ccggcggtca tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt
    11521 attcatactt ctgcttgtac cgttgcggta atgccagaac tacctgacgc agaactgccg
    11581 gacatcaacc cagcagattt acgcattgat actttccgct cgtcaggggc gggtggtcag
    11641 cacgttaaca ccaccgattc ggcaattcgt attactcact tgccgaccgg gattgttgtt
    11701 gaatgtcagg acgaacgttc acaacataaa aacaaagcta aagcactttc tgttctcggt
    11761 gctcgcatcc acgctgctga aatggcaaaa cgccaacagg ccgaagcgtc tacccgtcgt
    11821 aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta cttacaactt cccgcagggg
    11881 cgcgttaccg atcaccgcat caacctgacg ctctaccgcc tggatgaagt gatggaaggt
    11941 aagctggata tgctgattga accgattatc caggaacatc aggccgacca actggcggcg
    12001 ttgtccgagc aggaataatg gaatatcaac actggttacg tgaagcaata agccaacttc
    12061 aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct ggagcatgtt accggcagag
    12121 ggcgtacttt tattctcgcc tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac
    12181 ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat tgctcattta accggggtgc
    12241 gagaattctg gtcgttgccg ttatttgttt cgccagcgac cttaattccg cgcccggata
    12301 cggagtgtct ggtggagcag gcactggcgc ggttgcctga acaaccttgc cgtattctcg
    12361 atctcgggac gggtaccggg gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg
    12421 aaattatcgc tgtagatcgt atgcctgatg ctgtctccct ggcacaacgt aatgcccagc
    12481 atctggcgat caaaaatatc cacattctgc aaagcgactg gtttagcgcg ctagccgggc
    12541 agcagtttgc gatgattgtc agcaatccgc cgtatattga cgagcaggac cctcatcttc
    12601 aacaaggcga tgtccgcttt gagccgctca ctgcgctggt tgcggcagac agtggaatgg
    12661 cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc
    12721 ttctggaaca tggctggcag cagggcgaag cggtgcgaca ggcatttatc ctcgcggggt
    12781 atcatgacgt cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg ctcggccgct
    12841 attatcaatg acaagttttt ctacactgct tagtgttcat cttattagta tcgcgctttc
    12901 tgttgggcta ttaaccttac gtttctggct acgttatcag aagcatccac aggcatttgc
    12961 tcgctggacg cgcattgtgc cgccggttgt cgatacgttg ttattgttaa gcggcattgc
    13021 gttgatggct aaagcgcaca tcctgccatt ttccgggcag gcacagtggc tgactgaaaa
    13081 gctgtttgga gttatcattt atatcgtttt gggttttatt gcactcgatt atcgtcgtat
    13141 gcacagtcag caggcgcgca ttattgcctt cccgctggcg ttggtggtgc tgtacatcat
    13201 cattaaactc gccaccacaa aagtaccgtt actggggtaa gtcatgagat cgttagctga
    13261 tttcgaattt aataaagcgc cattgtgcga aggcatgatc ctggcttgcg aagcaatccg
    13321 ccgcgatttt ccctcgcaag atgtttacga cgaactggag cgtctcgtta gtctggcgaa
    13381 ggaagaaatc agccagcttc tgcctttaga agagcagctg gaaaaactga tcgcgctgtt
    13441 ttacggcgac tggggattta aagcctcacg cggcgtttat cgtctttccg atgcattatg
    13501 gctggaccag gtgttaaaga atcgacaggg cagtgcggta tcattaggtg cggttttatt
    13561 atgggtcgcg aatcgtctcg atttgccgct gctgccggtg attttcccta cgcagctgat
    13621 attgcgcatt gaatgtccgg atggcgaaat ttggctgatt aatcctttta acggtgaatc
    13681 gttaagcgaa catatgctgg acgtatggtt aaagggaaat atcagcccgt cggcggaact
    13741 gttttatgaa gaccttgatg aagctgataa cattgaggta atccgcaaat tgctggatac
    13801 actcaaagcc tcgttgatgg aagaaaatca gatggagctg gcgttacgca ccagcgaagc
    13861 tttattacaa ttcaacccgg aagatcccta tgaaattcgc gatcgcgggt tgatttatgc
    13921 gcaactggat tgcgaacacg ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc
    13981 ggaagacccg atcagcgaaa tgatccgtgc gcaaataaat aacatcgcgc ataaacatat
    14041 tgtgctgcat taattaatcg acattttact caaggttaag gcgatcctat gaaacaaaaa
    14101 gtggttagca ttggcgacat caacgtagca aatgacctgc cgttcgtact gtttggcggt
    14161 atgaacgtgc tggaatctcg cgatctggcg atgcgcattt gcgagcacta cgtaaccgtg
    14221 acccagaaac tgggtatccc ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc
    14281 tccatccact cttatcgtgg accgggcctg gaagaaggga tgaaaatctt ccaggagttg
    14341 aagcagactt ttggcgtgaa aattatcacc gacgttcacg aaccaagtca ggcacagccc
    14401 gttgctgatg tcgtggatgt gattcagttg ccggcgtttc ttgctcgcca gactgacctg
    14461 gttgaagcca tggcgaaaac cggtgcggta attaacgtca agaaaccaca gtttgtcagc
    14521 ccgggacaga tgggtaatat cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt
    14581 ctttgcgatc gtggtgctaa cttcggctat gacaacctgg ttgtcgatat gctgggcttc
    14641 agcattatga agaaagtgtc tggtaactcg ccggtgattt tcgacgtgac ccacgcattg
    14701 caatgccgcg atccttttgg cgcagcttcc ggcggtcgtc gtgctcaggt ggctgagctg
    14761 gcacgagccg gtatggcggt aggtctggcg gggctgttta ttgaagcgca tccggatccg
    14821 gaacatgcga aatgtgatgg tccatccgcg ctgccgctgg ctaaactgga accgttcctc
    14881 aagcagatga aagcgattga tgatctggtg aaaggtttcg aagaactgga taccagcaag
    14941 taatcttttt tgcttgaaaa ataaagtctt agcgttctgc gttaagactt ttttcatggg
    15001 tgccggatac aaaaaaggcc gcaggctgtt atccctgcgg ccggtttcgg gcgcatattg
    15061 ccatcacggc agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa
    15121 tcgctgcggt aataattccc gccaggatcg gtgctgccag gtcgtgccag aaaatcatgg
    15181 caaactgcgc gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct
    15241 tgcattgttg atggttcaag attagccccc gttctgttgt caggttttac ctctcaacgt
    15301 gcgggggttt tctctttcca gcaaccaatg ccaccaggga taaagccccc gcaacattgc
    15361 gcctcaccgg ataacgccgg cttggtgtgg atactacgac tcaattcatc ttcacttcat
    15421 ccctgaaatg tttgcaatga agagtgcatt ccggtttttc aacagctgtt acagtcgttt
    15481 tatgagtgct ctggatgatg cttccagctc gggttgccaa tatttacttg tggaagtgat
    15541 aaagacaaaa atggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat
    15601 cacggcagcc tgacgcccgt tttcacctta cttccggtta cgccaccagc caacaatcgc
    15661 tgcggtaata attcccgcca ggatcggtgc cgccaggtcg tgccagaaag tcatggcaaa
    15721 ctgcgcgagc gtcatataac cgccttgttg taatgacaac attttgcggc tattcttgaa
    15781 gtgtctggtt tcaagattag cccccgttct gttgtcaggt tttacctctc aacgtgcggg
    15841 ggttttctct ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc
    15901 accggataac gccggcttgg tgtggatact acgtctcaat tcatcttcac ttcatccctg
    15961 aaatgtttgc aatgaagagt acattccggc ttttcaacag ctgttgcagt ggtttcgtgc
    16021 aggttgtggt acaggctcgc aattctgatt aacgacctgt ccaggtagta tgaagaaata
    16081 caataagcag gaatttatat tatctccccc gatttccgtc atcggaggag atataactgt
    16141 caggcaaata tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc gccattgagc
    16201 acgttagtac gtccggtgga gaaggagata tggcacagca ctaaagaggc caccatcacc
    16261 accatttctg gcgcaccaag tgcaaactgc aattcgttac ccgtcataaa tgcaattagc
    16321 gtgacgacag gtacggtaag cgaaattgtt gctaacactg aaccaaagaa cagattcatc
    16381 gcgcgctgga cctggttgtt caacactgct tttaatgcac ctaaaccttc cggcgacaga
    16441 atcaacagtg ccaccaggaa gccagtaaag gcgacagggg cattcatgct gtcgagcaaa
    16501 gtctccagcg agctggcgtt cattttggtc accgcaataa cggcaatcag atggataatc
    16561 aaccagatag catgccacag gctgctatgg gcagacggtt taccgtggtg cgggtcgtcg
    16621 tcatcactgt catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt ggtctggatc
    16681 agcaaaaata cgccatacat tgccgcagaa attaatgcta ccagtaacgc ctgaccggtt
    16741 gaaaaattcg ccgcaggcag agccattgga aataccagta cgattatcgc cagggggaac
    16801 agcgcaatta aatactgctt gataccaaac agattcatat attgggtggc aaacttacga
    16861 ccgcccaaca ataatgaaaa gccaaccagc ccaccggtaa caatcataat gattgaatag
    16921 agcgtatcac gcattagcgt tggcgcggcg tcgccggttg ccattaaagc tgaaatcaaa
    16981 ctgacttcaa gaataaccac tgaaaggcta agaataagcg aaccgtaagg ttctcccagg
    17041 cgatgggcta atacgtccgc atgacggaca acactaaagg cgctacttaa aataccaata
    17101 agcgcaagaa gattgatggc aatgaccact ggtagtgtct ggctgcttcc ccacaggaac
    17161 agcactacca gcgccagaac cgggaaaata agcgaagtct ccttgtggcg ggtttttacc
    17221 gcctcttgag catttgacat tatggttatc cctttgcaga tgaatttgtc gaaaatgtaa
    17281 aaaataggta ggaaaaataa cagaaagtgt ctggatatcg gtaacatttt acgaaatttt
    17341 accccgctgt cgatttttta ctcattgggg cataaaaata agtaatacgt ttagacaatg
    17401 tttgttttag cgtttcttaa gaacagtctg acatgaaaat tcttatgttt tggcaagagt
    17461 agatattgtt gaccacactt aatgttcaac tttgtaaaag gagtcaacga tgccgtataa
    17521 aacgaaaagc gacctgccgg aaagcgtaaa gcacgttcta ccgtctcatg cccaggatat
    17581 ctataaagaa gcgttcaaca gcgcatggga tcaatataaa gataaagaag atcggcgtga
    17641 tgacgccagt cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga agcatgaata
    17701 tgccaaaggg gatgatgata aatggcataa aaaatcgtaa aaccggtagc ttagttaaag
    17761 ctattcgtgc ggtgttgcct tgcaagtggt ccgcggattg catattgtcc cgttagtggt
    17821 ttcaaaatga gcagtaaaaa tgtccggaag acaccaaaaa gttgtcgcag ggaagcatgc
    17881 agtggcggag gtgtaaggtg ataacgcgtg atttcttgat gaatgccgat tgtaaaacgg
    17941 catttggtgc cattgaagaa tcactcttat ggtcagcaga acaacgggcg gcttcgctgg
    18001 cggcgacgct ggcttgtcga cctgatgagg gaccagtgtg gatcttcggt tatggatcgt
    18061 tgatgtggaa tccggcactg gagtttaccg agtcgtgcac cggtacactg gttggatggc
    18121 atcgtgcgtt ttgcctgcgt ctgaccgccg ggcgcgggac tgcgctccag ccgggacgga
    18181 tgcttgcact gaaagagggc ggacgcacca caggcgtcgc ctatcgactg ccagaagaga
    18241 cgctggagca ggaactaacc ctgttgtgga agcgcgagat gattaccggc tgttatctgc
    18301 caacctggtg tcagcttgat cttgatgatg gacgcacagt aaacgccatt gtgtttatta
    18361 tggacccgcg acatccagaa tatgaatctg acactcgcgc tcaggtcatc gcgccgttga
    18421 ttgcggcggc gagcggtccg ctgggaacca acgcacaata cctgttttca ctggaacagg
    18481 agcttatcaa actgggaatg caggatgatg ggctgaatga tttgctggta tcggtaaaaa
    18541 aactgctggc ggagaattat ccggatggtg tgttgcggcc gggattcgcc tgagtaaact
    18601 tccctcatcg tggggcgtca gacgccccct caaacattaa aatgtgagca ctttatcggc
    18661 tgacagcgtc cattgcgcca gttccacaag agtaccgatt tccaccccat caatcagagg
    18721 aagtgtacta atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg gtacattctg
    18781 agcggtaagg atctccagca tttgctgaat gttgtagcct tcccctggtt tttgcccgcg
    18841 caacccggct gtgaccgcat cagacatgag gaacagacgc agatccagat tgctctcctg
    18901 ctctcgtaac gcaatggcca gccgcaagct gttaaacaag gattcgctcc cgtaaggtgc
    18961 gccattggca acgatcacaa ttttttgcat tatttactcc tgtattcagg gaattagaca
    19021 ctcatcttct atcttactgc ttctgcagcg tctgaccaat cggtcacatt tttaaggatt
    19081 ttcctgaaag cgcgagaaaa tacgacaaaa gttgccagta atcgttattc tttaaggcta
    19141 tggtttttca ttttttaccg gaagttaccg acgttttgag ccgtttcgtt cctcgcatta
    19201 ttccgtttta tttactcttg ctggcggcag gcggtacagc taacgcacaa tctaccttcg
    19261 agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat ttaggcatgg
    19321 cacctgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat ttcggcgaaa
    19381 ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc gcattgggaa
    19441 aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcctgg ctatcaccgt
    19501 ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg acatttcacc ggcagtcgtg
    19561 gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc cagcttggtc
    19621 ttactcagca ggatgatggg ttggtgagca atgtgggcgt tgggcaacgc tgggcgcgcg
    19681 gcaactggct ggtgggttat aacacttttt atgacaactt gctggacgaa aatcttcagc
    19741 gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg actatcggca aacttttatc
    19801 agccatttgc tgcatggcat gaacagacag ccacgcagga acaacggatg gcgcgcgggt
    19861 acgacctgac agcccggatg cgcatgccgt tctatcaaca cctcaatacc agtgtcagcg
    19921 tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt tatcacaatc
    19981 ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact gtgacggccc
    20041 agcataaaca gggtgaaagt ggcgagaatc aaaataacct cgggctgaat cttaattacc
    20101 gctttggtgt accgctcaaa aaacaacttt ctgcgggcga ggttgccgaa agtcagtcgt
    20161 tacgtggtag tcgctatgat aatccgcagc gaaataatct accgactctt gagtaccgac
    20221 agcgaaaaac gttaacggtg tttctggcga caccgccgtg ggatctaaaa cctggcgaaa
    20281 cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg atttggcagg
    20341 gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg gagggctgga
    20401 cgctgatcat gcctgactgg cagaacgggg aaggggcgag caatcactgg cgattgtcgg
    20461 tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg ctaacgcttg
    20521 tcgaaccgtt cgacgcattg tcaaacgacg aactgcgctg ggaaccgtaa tcagaaaatg
    20581 cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat tttcttcagc
    20641 atgtgcttga cgtgcacttt tactgtgctt tcggtgatgt ccaggcggcg ggcaatcatc
    20701 ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgggg ggttaactgg
    20761 ttaacatcgc gctcagtcgt ggcacggtta gcgcgcaagc tggcggccag aacaggcgtt
    20821 aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt cagcagatct
    20881 tccggttcca tatcttttaa cagatagcca tccgcgccgc gtttcagtgc ggtgaccaca
    20941 tcttcttcat ggttagagac actgaatacc acaatgcgcc ctgagaggga cttttcgcgc
    21001 agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa caggatcaga
    21061 tcgggatcaa gagattccgc cagttcaata ccctgttcgc cattactcgc ttcgccaacc
    21121 acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag catcgggtga
    21181 tcgtcaatca gcaggatagt agccggttcc tgattactca tgggtatctc cttggacgtc
    21241 tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg attcacgacg
    21301 gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta tcatgccgta
    21361 gtgattgctg cggatggcat tttcaggcac gccgcagccg ttatcctgga cggtcagttt
    21421 gacctgatta tcgttttgcg ccaccgtcac cacgacctca ctcgcttgcg aatgtttgag
    21481 ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat gcgaaggcac
    21541 cagacgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg cgctgtactc
    21601 ttcgcaactc gcctccagcg ccggacgtaa tccaggctcg gtgagctgca agcggaatgt
    21661 ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga tctgacttaa
    21721 cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac tcacctgcat
    21781 cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa tggtggcacg
    21841 ctcttccatc acgatcaact gttgctgacg ttcctgatgc cgatccagcg ccagcgtggc
    21901 ggtgagttgt tcaaccaggg tatccaccag ttgttgttga tcatggctaa gatgacgccc
    21961 ctgcggcagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca gccgccactt
    22021 cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct ggcagccttt
    22081 atcatcacaa gtcatatctg gctggcaggt aaactcctga tgattctctt catcatcagt
    22141 gtcatacacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc cgttgagtac
    22201 aggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa cggcggttag cctgccataa
    22261 aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa cccgctgctc
    22321 aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag ttccaagcat
    22381 cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat ggctgacggc
    22441 actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca accagataat
    22501 agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca gtaccactgt
    22561 ctcaatacgc atttccgtgg tgcggtcaaa accagatacc agctgatcaa gcccggcaac
    22621 aaactggctg acatccgctg ataccgtttc tcggttttgc gcccgcatca gcgcagggat
    22681 cagttcatta cgccagtaat cttgtaaacc ctgtaattgc gccagttgtc cgtctcgttc
    22741 tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa ttaagggctt
    22801 gtctttctcg cttaatggca ctgctgccaa cagacggtaa ctttgcatgc gcagcgatcc
    22861 cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc cagaaaccgc
    22921 catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct gattaaccag
    22981 ggtgagcgga gagagacaac gtttaagcat gtaaacctct tccttcaggc tttaaatgag
    23041 caataacctt aatgaatgtg acgatacatt ctggaatggc agtattctcg gctattggct
    23101 gaagtatacc catacccgga aagagttact ccttatttgc cgtgtggtta gtcgctttac
    23161 atcggtaagg gtagggattc tacagcaccg tgaaaaatct cataattttt atgaaatcac
    23221 tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt atcgtttgat
    23281 ttacatcaaa ttgcctttag ctacagacac taaggtggca gacatcgaaa cgagtatcag
    23341 aggtgtctat gagtcactca tccgcccccg aaagggctac tggagctgtc attacagatt
    23401 ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc agccgcaacc
    23461 tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg ttcagcgctg
    23521 ttgcggtgaa cttaccgaaa gtcggtttta attttacgac cgatcagcta tttatgttga
    23581 ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt atggttccta
    23641 tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt ccttgtgtct
    23701 ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc atcatctctc
    23761 tgctgtgcgg ctttgctggc gcgaacttcg cttccagtat ggcaaacatc agcttcttct
    23821 ttccgaaaca gaagcagggt ggcgcgctgg gcctgaatgg tggtctgggc aacatgggcg
    23881 ttagcgttat gcagttggtt gctccgctgg tggtatcact gtcgattttc gcagtatttg
    23941 gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat gcgtcctgga
    24001 tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg gtttggcatg aacgatcttg
    24061 ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt catctgtgga
    24121 ttatgagcct gctgtatctg gcaaccttcg gctcctttat cggcttctcc gcgggctttg
    24181 cgatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct ttcttcgggc
    24241 cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt ctgggcggaa
    24301 ctcgtgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg ctgttcctga
    24361 ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc ttcctggcgc
    24421 tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttca gtgatcttcc
    24481 gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt gcgatgcgtg
    24541 aagcggcaac cgacacggcg gcggcgctgg gtttcatctc tgcgattggc gcgattggtg
    24601 gcttctttat cccgaaagcg tttggtagct cgctggcatt aacgggttcg ccagtcggcg
    24661 caatgaaggt atttttgatt ttctatatcg cctgcgtagt gattacctgg gcggtatatg
    24721 gtcggcattc taaaaaataa atcttaatat ttgattatcc tttgcgcggc atgatgtcgc
    24781 gcttttttta tgcgtcattt agttacaaca tactaatgtt atatggttta tttcgccgga
    24841 tttcattaag agccattaa
//
LOCUS       DAZGCQ010000074        24812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000074 DAZGCQ010000000
VERSION     DAZGCQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.074"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..985)
                     /gene="melR"
                     /locus_tag="ACHK07_003305"
     CDS             complement(77..985)
                     /gene="melR"
                     /locus_tag="ACHK07_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO8103595.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1268..2623
                     /gene="melA"
                     /locus_tag="ACHK07_003306"
     CDS             1268..2623
                     /gene="melA"
                     /locus_tag="ACHK07_003306"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO8103596.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2726..4147
                     /gene="melB"
                     /locus_tag="ACHK07_003307"
     CDS             2726..4147
                     /gene="melB"
                     /locus_tag="ACHK07_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO8103597.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4286..4915)
                     /gene="yjdF"
                     /locus_tag="ACHK07_003308"
     CDS             complement(4286..4915)
                     /gene="yjdF"
                     /locus_tag="ACHK07_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO8103598.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5038..6684)
                     /gene="fumB"
                     /locus_tag="ACHK07_003309"
     CDS             complement(5038..6684)
                     /gene="fumB"
                     /locus_tag="ACHK07_003309"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8103599.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6762..8102)
                     /gene="dcuB"
                     /locus_tag="ACHK07_003310"
     CDS             complement(6762..8102)
                     /gene="dcuB"
                     /locus_tag="ACHK07_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO8103600.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8673..9392)
                     /gene="dcuR"
                     /locus_tag="ACHK07_003311"
     CDS             complement(8673..9392)
                     /gene="dcuR"
                     /locus_tag="ACHK07_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO8103601.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9389..11020)
                     /gene="dcuS"
                     /locus_tag="ACHK07_003312"
     CDS             complement(9389..11020)
                     /gene="dcuS"
                     /locus_tag="ACHK07_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO8103602.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11201..11431
                     /gene="yjdI"
                     /locus_tag="ACHK07_003313"
     CDS             11201..11431
                     /gene="yjdI"
                     /locus_tag="ACHK07_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO8103603.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKCRHK"
     gene            11443..11715
                     /gene="yjdJ"
                     /locus_tag="ACHK07_003314"
     CDS             11443..11715
                     /gene="yjdJ"
                     /locus_tag="ACHK07_003314"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8103604.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11942..12238
                     /gene="ghoS"
                     /locus_tag="ACHK07_003315"
     CDS             11942..12238
                     /gene="ghoS"
                     /locus_tag="ACHK07_003315"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8103605.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12266..12439
                     /gene="ghoT"
                     /locus_tag="ACHK07_003316"
     CDS             12266..12439
                     /gene="ghoT"
                     /locus_tag="ACHK07_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO8103606.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12558..14075)
                     /gene="lysS"
                     /locus_tag="ACHK07_003317"
     CDS             complement(12558..14075)
                     /gene="lysS"
                     /locus_tag="ACHK07_003317"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8103607.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGDRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14312..15769)
                     /gene="dtpC"
                     /locus_tag="ACHK07_003318"
     CDS             complement(14312..15769)
                     /gene="dtpC"
                     /locus_tag="ACHK07_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO8103608.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15828..17975)
                     /gene="cadA"
                     /locus_tag="ACHK07_003319"
     CDS             complement(15828..17975)
                     /gene="cadA"
                     /locus_tag="ACHK07_003319"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO8103609.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18055..19389)
                     /gene="cadB"
                     /locus_tag="ACHK07_003320"
     CDS             complement(18055..19389)
                     /gene="cadB"
                     /locus_tag="ACHK07_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO8103610.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19754..21292)
                     /gene="cadC"
                     /locus_tag="ACHK07_003321"
     CDS             complement(19754..21292)
                     /gene="cadC"
                     /locus_tag="ACHK07_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO8103611.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(21552..21666)
                     /locus_tag="ACHK07_003322"
                     /pseudo
     CDS             complement(21552..21666)
                     /locus_tag="ACHK07_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(22103..22945)
                     /locus_tag="ACHK07_003323"
     CDS             complement(22103..22945)
                     /locus_tag="ACHK07_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8103612.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(23030..23227)
                     /locus_tag="ACHK07_003324"
     CDS             complement(23030..23227)
                     /locus_tag="ACHK07_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8103613.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRILRQLQFLQQNR"
     gene            complement(23247..23735)
                     /locus_tag="ACHK07_003325"
     CDS             complement(23247..23735)
                     /locus_tag="ACHK07_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8103614.1"
                     /translation="MKLTLTLEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(23732..24109)
                     /locus_tag="ACHK07_003326"
     CDS             complement(23732..24109)
                     /locus_tag="ACHK07_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8103615.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            complement(24156..24530)
                     /locus_tag="ACHK07_003327"
     CDS             complement(24156..24530)
                     /locus_tag="ACHK07_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8103616.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            complement(24693..>24812)
                     /locus_tag="ACHK07_003328"
     CDS             complement(24693..>24812)
                     /locus_tag="ACHK07_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO8103617.1"
                     /translation="TEAYTGREVQDIPGVLAVFAERHKDSFGPYVRLMSVTLN"
BASE COUNT         6357 a         5853 c         6106 g         6496 t
ORIGIN      
        1 tgtgattatt ttaaattatt ttcactgctt attatcgacg gctaaactat ttttttggct
       61 gatactgata tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt
      121 attgttgcgg cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac
      181 gaaagcctgc cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc
      241 gaacgtggtt gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct
      301 ggaatatccc cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg
      361 tcagcgcctg atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg
      421 catggcgcga gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc
      481 agccagagag gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa
      541 tttgctcgtt cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt
      601 gctgtgtggc cagtgatttg atcaccatgc cgtgagtgac gtggttaatc aggtctttat
      661 ccagcggcca ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg
      721 ttccggtatc tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat
      781 tgatattcac tttttcattg ttgatcaggt attccacatc gccatcgaaa ggcacattca
      841 cttcgacctg accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct
      901 ccatccgctg gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc
      961 tgcacataaa cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg
     1021 caggagtgag ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg
     1081 cgaggggagc gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc
     1141 ataaactcag atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg
     1201 gaagccggag gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga
     1261 gatctgcatg atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt
     1321 taaaaatatt cttggtgatg tgttccatcg cgaggcgctg aaaacggcgc atattgccct
     1381 gatggacatt gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agctgatgga
     1441 ttcagcaggg gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga
     1501 tgccgatttt gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga
     1561 tttcgaggtc tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg
     1621 cggtattatg cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac
     1681 ggaagtctgc cccgatgcca ccatgctcaa ctatgttaac ccaatggcga tgaatacctg
     1741 ggcgatgtat gcccgctatc cgcatatcaa acaggtcggg ctgtgccatt cggtgcaggg
     1801 aacggcggaa gagctggcgc gtgacctcaa tatcgaccca gctacgctgc gttaccgttg
     1861 cgcaggtatc aaccatatgg cgttttacct ggagctggag cgcaaaaccg ccgacggcag
     1921 ttatgtgaat ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc
     1981 gaatattcat ggcaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct
     2041 gggctacttc gtcacggaat cgtcagaaca ttttgctgag tacacaccgt ggtttattaa
     2101 gccaggtcgt gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg
     2161 ctgcgtcgag cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg
     2221 gattgatatt aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg
     2281 cgagccgagt gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca
     2341 aggatgttgc gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt
     2401 cggtacgcta ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct
     2461 gctgaccgaa gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga
     2521 cccgcatact gctgccgtgc tgggcattga tgaaatatat gctcttgttg acgacctgat
     2581 tgccgcccac ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta
     2641 ctgcgccggg ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc
     2701 gttggtaaca gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg
     2761 atttggagcg ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta
     2821 ctacaccgat gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag
     2881 gatctgggat gctattaacg atccgattat gggatggatt gtaaatgcta cgcgatcgcg
     2941 atggggtaag ttcaaaccct ggatcctgat cggtacgttg gcaaactctg taatcttatt
     3001 tctcctcttt agtgcgcatc tgtttgaagg tactactcag attgtctttg tttgcgtgac
     3061 ctacatcctc tggggcatga cttacaccat tatggatatt cccttctggt cgctggttcc
     3121 aaccatcacg ctcgataaac gtgagcgcga acaactggtt ccttatccgc gtttttttgc
     3181 cagtctggca ggctttgtta cggcaggtgt gacgctacca tttgttaatt atgtcggcgg
     3241 tggcgatcgg ggatttggct ttcagatgtt cactctggta ctaatcgcct tttttattgt
     3301 ttcaaccatc atcactctgc gcaatgtgca tgaagtcttt tcgtcagaca atcaaccgtc
     3361 tgctgaagga agccatctga cacttaaagc catcgttgcg ctaatttata aaaacgatca
     3421 gctttcatgc ctcttgggta tggctcttgc ttataatgta gccagcaaca ttattaccgg
     3481 atttgctatc tattatttct catatgttat cggtgatgcg gatttgttcc cctattatct
     3541 gtcgtatgcg ggagctgcta acctggtgac gttagtattc ttcccacgct tagttaaatc
     3601 attatcccga cgcattttat gggccggagc atctattctt ccggtgttaa gctgtggtgt
     3661 tctcctgtta atggcattaa tgagctatca caacgtcgtc ctcattgtga ttgcgggtat
     3721 tttgctgaat gtgggaacgg cgcttttctg ggtattacag gtcatcatgg tggcagatac
     3781 cgttgattac ggtgaatata aactgcacgt acgctgtgaa agtatcgctt actccgtgca
     3841 gactatggtg gtgaagggcg gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg
     3901 gatgattggc tatgtaccga atgttgaaca gtctacgcaa gccctattag gtatgcagtt
     3961 tattatgatt gctctaccaa ctctgttttt catggtaacg ctgattctct acttccgttt
     4021 ctatcgcctc aatggcgaca cgctgcgcag gatccagatt catctgctgg ataaatatcg
     4081 caaagtaccg cccgagcctg ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa
     4141 agcctgacgt ggacaatagc taacgaaaat ggcctgatgc gatatgttta tcaggccacg
     4201 tcttaatgta atgctttgga tttgcaaatt ttgtagaccg gataaggaat tcacgccgca
     4261 tccggcatca acaaagcgca cgttgttatc cggttatcaa gccaaagcgc cgtagctggc
     4321 ggcaatgaaa gcgagcgagg aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca
     4381 tatcggattg cgtgtcccac tggtcgccct gggtgccgag aaaatcatcc gctccctggc
     4441 ccattgccag cgccgcccac cactcgatta attcatacat cgcgcttatc gccagcgcca
     4501 cgcagcagac caggaacgcc accattttac gtccgcgcac gtacatcccg cgcacgagaa
     4561 tttctcgtgc caccagtgca ggcaccagcc cctggaaaaa atgccccagc ttgtcatacg
     4621 gattacggct caaccccagc cattcctgca cctcaaaacc gatggggact ttcgcgtagg
     4681 tgtattgccc gccgaccatc aggatgatgg cgtgaaagaa aatgagcgta tagagcagcg
     4741 gcgttaacgg ataacgttta gcggtggcaa gcagtagcgg cacgacaata atcaccggtg
     4801 tcacttccat cagccaggtg agtttgtcat gggccgaaat gccggtataa atcaggatta
     4861 acgttagcgc cagtgcgccg gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga
     4921 gttcgcaagt cagggaaaat actgactatt caccagcaag cgtgaaatta caatcgcaaa
     4981 agaggtggcc cagggggatc acctggcagc atgctgccag gcgctgggcc gaagaggtta
     5041 cttagtgcag ttcgcgcact gtttgttgac gatctgctgg aagaagtcgt tacctttgtc
     5101 atcgaccagg ataaacgccg ggaaatcttc tacttcgatt ttccagatag cttccatacc
     5161 cagctccgga taagcgacgc attccagatg cttgatgctc tgctgcgcca gtaccgccgc
     5221 cggaccgccg atgctaccga ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg
     5281 ctgactgcgg ttacctttcg ccagcatgat catgctgccg ccgtgggatt gcagcagatc
     5341 cacgtaggag tccatacggc ctgcggtggt tgggccaagt gaacctgatg gataaccggc
     5401 aggggttttc gccggacccg cgtagtagat cgggtgatct ttgatgtact gcggaagttc
     5461 tttaccggcg tcaatcagct ctttcagctt ggcgtgtgca atatctcggc ccacgataat
     5521 ggtgccggtg agcgacaaac gagtggatac cgggtattgc gaaagctggg cgaggatctc
     5581 tttcatcggg cggttaaggt caactttcac cgcttcgcct tcaccggcct ggcgcagttc
     5641 ttgtggaatg tactggcctg ggttgtgttc cagtttttcg atccagatac cttcgcggtt
     5701 gattttcgct ttaatgttac ggtcagcgga gcaggagacg cccatgccga ccgggcagga
     5761 tgcgccgtga cgtggcagac ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa
     5821 ctgcgcgcca agaccgagtt tctgggcctc ttccagcagt tcctgttcca gctggacatc
     5881 gcggaacgcc tgaccatgtt cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct
     5941 tgctaacttg acggttttca ggttggtttc cgcagacgta ccgccaatca caaacgcgat
     6001 atggtacggc gggcaggctg cagtaccgag ggtacgcatt ttctcgacga ggaagttttt
     6061 cagtttgccg ggagtcagca gggctttggt ttcctggtag agatacgttt tgttggcaga
     6121 gccgccgcct ttcgcaacgc aaaggaattt gtactcatcg ccatctaccg cgtacaggtc
     6181 gatttgcgca ggcaggttag tgccggtgtt gacctctttg tacatgtcca gcgccgcatt
     6241 ctgtgaatag cgcaggttat cttcgatata ggtgttatag acgcctttcg acagcgcttc
     6301 ttcatcaccg ccgccggtcc acacgcgctg gcctttttta ccgacgatga tcgcggtgcc
     6361 ggtatcctgg caggtcggca gcacgccttt ggcggcgatt tcggagtttc ttaagaattg
     6421 cagcgccacg tacttgtcgt tttcgctggc ttctggatcg tgaagaatag ccgcaacctg
     6481 tttctggtgt gccgggcgga gcataaaaga agcgtcgtga aaggcttgct gcgccagcag
     6541 ggtcagggct tctggttcca ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac
     6601 gtaatcggaa gtgagtagat agtattcggt attgtctttc cccatcggga aaggtgcctg
     6661 gtagataaag ggtttgtttg acatagcttc cagcctgtaa ctctgtattt gttattcgaa
     6721 aatggcgtgc cgcgtagtca cgggcggcac gcaaagtgca tttataagaa cccgtacatc
     6781 gcggcgaaga tccagccgaa gacgcacgat acgctcacac caatcaaccc cggcagaata
     6841 aagctgtggt tgatgacgaa gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc
     6901 gctgccagat cgctcggata agtcggcagg atgtaataac cgtagcaagc cggtgctgaa
     6961 gccacgatgt atgccggatc aacgccgatc gccagcgcga ccggaacaat cgccgccagc
     7021 gccgcagcct gagagtttac aaacttggaa accagcagca gaacaatggc ataggcccac
     7081 ggatactctt tcaccatttc acccagtacg ccctgaattt cagacatatg cgcaccgaac
     7141 atggtttctg ccatccatgc gataccgtac accgccacga tggcgatcat accggaacgg
     7201 aagacttcgt tttttgagat agacgcggga ttggttttgg tcaggataat aatcagcgcc
     7261 ccggtcagca gcataaacat ctgaataacc agtaccatcg acagcggttt gccgccgaag
     7321 gatggacgca ggtccgaatc agcaccaaga agtgcgacta cagcgattgc accgaggaaa
     7381 atccacattg ccagccagtt gcttttcggc agttttttat cgagcagcgt cgcggtatca
     7441 ccgtaaacat actcacggtt ttccggtacg gagatgaatt tctggaactc ttcgtcttta
     7501 tccagatctt taccgcggaa ccagctgaag ataccgatcg ccaggatacc gattaacgtc
     7561 gatggaatgg tgattgccag caggtcgagg aactcaagat ggcgaccatc aaaggtgaca
     7621 ttacccagca ttgcaaccag agacacgacc gcaaccgaca ccggactggc gataatcccc
     7681 atctgtgcac cgatagaact tgccgccatc ggacgttccg gacggatgtt gttcttaatg
     7741 gcgacgtcgt agatgatcgg cagaatggtg taaaccacat gacccgtacc gcaaagaatg
     7801 gtcagtgtac aggtcacaaa cggcgcgaca attgagacat atttcgggtt gcggcgcagc
     7861 aacttctcgg caatttgcag catgacatca agaccgcccg aagcttgcaa ggtcgccgat
     7921 gccgccacca ccgcaatgat aaccagcatg acatcaactg gtggtttacc tggctgaagg
     7981 tggaagacga agaccagaat gaccagaccg ataccgccta ataaacccag cgcgatacca
     8041 ccctttctgg caccataaaa cagacatatc agtattatga taagttggat agtaaataac
     8101 atgtgtgaac cctcgcgata atcctattta aatttttgct gaatagatca cagtcacgtt
     8161 ctgttttgta tgaactgttt tcagagcctg actaaatatc cgtttggtcg ttaccggctt
     8221 tagcaaatac ctcacagtga atattggctg attaaagcag cggcaaattt atgcactgtg
     8281 ataagcggct ttttcagaga gaaagactct ctggcgatgg gtttatagtt tgtactgcgc
     8341 agatgttaat gttttgtttg ctgttatctt attgatattt atcggtctat tttttgttct
     8401 cagggcagat gtcattaggt ttatagatta atctgatcta cccatttgtg ggtaaaaata
     8461 cacataatgc gggtgacata atagttaatt aacttttgtt agcgttttga aattaaaaac
     8521 accgttcacc tgaagagata ttaattttta gcgatgatgg agggataatt atatttgatc
     8581 tggcacaagt tttactgatg aaggatgtaa cttgtgccag gggtattttg cattacggta
     8641 ataattatta cttacagata accagcgatc cgttattggc aatattgttt cagtagtgag
     8701 tagtgttctg cctgaatacg gtaacggtaa actggacgcc ccgtgacgcc ataatggata
     8761 ctggtgaaca agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc
     8821 gagatgttaa cctcgttagc cagctcgtcg gttgaaaact catagtcctg atgcgcgtca
     8881 atccactggc acagcgtgcg taacgtctgc ggcgttaagc cttttggcaa gcgacgagga
     8941 tcctgttcgt tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac
     9001 tgatgttttt ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg
     9061 gaagcctgga agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg
     9121 gttgccgcat cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc
     9181 aggacaggca gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg
     9241 tcgataggcg tatcgctatt gaagataatc tctttggctt tctccagcgt cgaggctgtt
     9301 ccacagcatt gaaagcctgg gatttgtgct acgtatcggc gattcagctc cgcgaccatt
     9361 gcgtcgtcat cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta
     9421 tctggacaaa aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga
     9481 gattttctac ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg
     9541 agacaccttt gtcaaaaatg tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt
     9601 taacttcgca gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg
     9661 gttccggccc taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg
     9721 tcgcgacttg gtcctcactg ccgctgtctg gcagcaggct ttcactgttt aaaatcagcg
     9781 tatggcctaa atcggtcgcg cggttaatct tgctgattaa aaaaccagcg ataaccggag
     9841 atttgatctt acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa
     9901 tgtaatcttc caactgctta taactcttca gatgcaataa tccgagaatc acatgcaatt
     9961 tattcataaa ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc agaccgtcga
    10021 gtcgctgcat cagtttacga acttcagttt tgtccctgaa ggttgaaatg gcaccgatga
    10081 taacgccatt actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa
    10141 tctcttcgtc gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct accacctgtg
    10201 accatgagtg gcttagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca
    10261 attcttgtgc ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc
    10321 cttctttgat agactgcaac atggcctggc gctgctcaaa cagcgtggag atttcgtagg
    10381 gttccaggcc gaaaaggatt tttttcagta ccttaaccag aatgcaggtg ccaatcagtc
    10441 cgaccagcat gccaaataat accgaccaga taatgctcca gcgactgtca ttgatttgtt
    10501 gggtcacgcg gcttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat
    10561 cgtagatggg ggtaaatacg cgtaaagcct gtgccagaaa accgcgattg atagcgacat
    10621 tttctttgcc attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct
    10681 gggcttcagg atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat
    10741 cgttgcgttt gcgcacggct tccgcgatgg cctggattcc gctctcctgc ggttttttct
    10801 gcaagccctg acggatttcc ggcgagtcgg cgagggtacg cgccattgcc agtgccttgt
    10861 tggccagccc gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca
    10921 atagcaccga gaacagtacc gcgctgacca ttaagatcac tgtggtactc aatttcatcg
    10981 gacgtttgcg taacatgcgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa
    11041 tttcttaagc attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta
    11101 tataggggaa gagactctgg caacggaaac tgccagtgct gtatgaagat tccagggcta
    11161 tgcttatagc gataatcata ctgatgagag agggaaagtc atggatcagg cgctactgga
    11221 cgagggttat cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg
    11281 tcagcattct ggcaattgcg tacgtggcaa cggcaagtta tttaatctca aacgaaagcc
    11341 gtggatcatg ccggatgaag tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc
    11401 gagcggcgcg ctgaaatgcc gtcataaata agcgagggta aaatggaaat acgcgaaggc
    11461 cacaataaat tttacattaa tgacgaacag ggcaagcaaa tcgctgaaat tgtctttgtg
    11521 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
    11581 caggggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
    11641 aaaattatcc cactatgccc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
    11701 gatattcgca gttgatggga gagtacagag tcacgatatt tttcatgctc tccgcggagt
    11761 gatgcaggag agcatctgaa gggtaggggg atgcacaaag aatgggcaga gagcgcgttt
    11821 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
    11881 atcaatcggg gttaataagt tttgcgtccc cagagcattc aatattgata ggagtcatat
    11941 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
    12001 ttcctcagtg ttcttaaggt taagatcatt gatgtatgat atgaatttct cctctatcgt
    12061 ggctgatgaa tatgggatac cacgacaatt gaatgaaaac tccttcgcaa taacgacatc
    12121 gttagccgca agtgaaatcg aagatttaat caggcttaaa tgcttagact taccggatat
    12181 tgattttgac ctcaacatta tgacagttga tgactatttc cgtcagtttt acaagtagcc
    12241 aggaaggata agaagaacat aaaaaatggc actattctct aaaatattaa ttttttatgt
    12301 gattggtgtg aacatatcct ttgtcattat ctggtttatc tcacatgaga aaacacatat
    12361 tcgtttactt agtgcattcc tggtcggaat aacctggcca atgagtctgc ctgtggcatt
    12421 acttttttct ctcttttagg agctgtagtt gagtggaccc gctggttatc gtgaaacgaa
    12481 gcgtctctcc tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc tgactgcttc
    12541 gttcattaaa gtgaaattta tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga
    12601 atagtatgac tgtttgtaaa cagcataatc attcggtcga taccaatacc cagaccagcg
    12661 gttggcggca gaccatattc cagcgcagtc acgtaatctt cgtcatagaa catggcttcg
    12721 tcgtcacctg cagctttagc gttaacctgt tcctggaaac gttcagcctg atcttctgcg
    12781 tcgtttaatt cgctaaaacc attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg
    12841 tcggtgattt ccgggttaac atcattacgg cgcgccagcg gtgacacttc tgccggatat
    12901 tccgtaataa aggttggctg aatcagatgt gcttctgcca cttcatcaaa gatctccgtg
    12961 acaatacgtc ccagccccca gcttttctct accgtaattc cgatagattc agctaatgct
    13021 ttagcagcat caaaattatc caggtcggcc atatcggttt ctggacgata ttttttgatt
    13081 gcttcgcgca tggtgagttt ttcaaacggt ttgccgaaat caaacacatg ctcgccataa
    13141 gtgactttag tggtacccag aacctcttgt gccagggtgc ggaacaggga ctctgtcagt
    13201 tcaatcaaat cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt gaactcagga
    13261 ttatggcgaa cagaaatacc ttcattacgg aagttacggt tgatttcgaa tacccgttca
    13321 aaaccgccta caaccagacg tttcagatac agctccggcg cgatacgcag gtacatatct
    13381 aaatccagag cgttatgatg ggtaataaac gggcgagcag atgccccacc tggaattacc
    13441 tgcatcatcg gggtttctac ttccataaag ccgcgcgcga ccatgaactg gcggatagcg
    13501 gccagaattt ttgaacggac aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc
    13561 agataacgtt gacgataacg gacttcctga tcctgcagac catggaattt atctggtaaa
    13621 ggacgtagtg cttttgtcag caggcgcaac tcagtacagt gaatggaaag ctcaccggtt
    13681 tgcgtcttaa acagcgtacc gcgggcaccg ataatgtcac ccagatccca ttttttaaac
    13741 tgatcgttat aaacaccttc tggcaggcta tctcttgcaa cgtacagttg aatacggtca
    13801 ccgacatcct gcaacgttac aaaggaggct ttccccatga tacgacgggt catcattcgg
    13861 ccagcaaccg agacttcaat gtttaaggat tccagttcct ggttatcctt cgcatcaaac
    13921 tcttcgtgca gctggtcaga ggtatggtcg cggcgaaaat cattgggaaa cgccacacct
    13981 tgctgacgta gtgccgccag tttttcgcgg cgatttctca gttcatcgtt aaaatcaata
    14041 gcctcattgg ctccccgtgt ttcttgttca gacattttgg ttcctctaaa tccagctttc
    14101 aatttagcgt agataaagag acagatcggt acactgtatt atctgcctcg actattaata
    14161 actcagttta tcttatcgaa actaacgctt acctcaagtt gatatcacta ataaataacc
    14221 atcaaaatgg tttaaatgac ttattaatct agatttgcta gtgttataaa ctgaaagtta
    14281 atttattcat aaccaattgt ttttacgaca gttaatcgtt gctctcctgt atcatattcg
    14341 tcggcgtgct aaacagaaaa cgggtagcaa aggccaatac gacgatgatc gcgacacaag
    14401 ccaacgtcca ctctcccatc tgagaaaaga atcgctggta agctgcaatt gccataccgc
    14461 taatttgcga ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc
    14521 ctgttgccag catataaata ccggttaata cgccagacat tttcagacgc gtgatttgcg
    14581 caatcgccac cgggtcaata aagagttcgg caaagcccat tagcgccaac ccggatatca
    14641 tcacgcccat tgacgcttga ccgtcagccg ctgcatgtcg ggcatcaaat gccaacaaca
    14701 taaagccaca agccatcagc agtaagccaa aggcaaactt cagccagacg cgcaatgttg
    14761 agttgccgcg gctttctggc gacgccagcc aggccagtac aaccccagcg agcatcaccg
    14821 caattgcatt caccgactgg aatagtgctg taggtacttc aatattgaat gcctgacgat
    14881 tcacaaagcg atcgataaac aagctgatgg tactgccgcc ctgttgtgcc agtacccaga
    14941 acaatgtccc gacaaacatc aacaatacaa tttgccaaag agcacggcgg tgttcgggga
    15001 atttgaccat catgcgggca atgatttgtg cggcaatgag gcaaacgatc gccagcaaat
    15061 atccggacca gtcgttctcc agcagcaggg taaaaaatac tggggctaaa cagagcatca
    15121 ccactaacca gctccatact ggtaaggcaa atttcacgct agtgagcgct tttttatcca
    15181 tactacgcgt ggactggaaa tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca
    15241 tgccgccacc cgcaagggca aagccaacat gccatccata ccactgagca gccaggccgc
    15301 aggcgattgg ggcggcgata gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc
    15361 catcacgtcg atgatcgttc tcgtcgtaga gctcgccaag caaacagctg atgtttgatt
    15421 tgaataaacc gtagccacaa ataatgattg ctagcgccag atacaggcta aaggttgaat
    15481 ttgtatcaat gcccagcacc acatggccaa gggtcattaa cagcgcgccg gcaatcactg
    15541 cagtgcggtt gccgagcagg cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa
    15601 ccagagaagc atatgcgctg aacaggctga tggcatggtt atcatcaaaa ccaagctgat
    15661 gggtgagata gagaatgagt aaggcacgca tgccgtaaaa actgaagtac tcccagattt
    15721 ggatcgccac gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt
    15781 atgagcaaaa aagggaagtg gcaagccact tcccttgtac gagctaatta ttttttgctt
    15841 tcttctttca ataccttaac ggtatagcgg ccatcagcct gacggtatgc accgtgaata
    15901 tcggtttcaa agcccggata gtgagcgccg atttcacaca gcatctgcag gaactccaga
    15961 accggacggc tttcttcggt gatcatttca cccggcatta ccagaggaac tcccggcgga
    16021 tatggaagga tcatattggc gttaatacga cctaccattt cgtcgaggta aacttcttcg
    16081 gtcataccgt gcagctcttt ctggaacgca gcatacggag tcattaccat cgtcggcagc
    16141 acttcaaatg cgcggtacat cagatccggc agattgtggt gaacaatcag tttgtggata
    16201 ttctgagcca gttcctgaat acgcatgttt tcatagaatt caggatcttc acgatacaga
    16261 gacggcagca tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga
    16321 gcacgcagca ggctcagtgc tttggtctta tcgataccga tgctgaacag gaacagcagg
    16381 ttatacggac cggttttctc aacaacgatg ccatgttcgt cgaggtattt cgccacgatg
    16441 ctggccggaa taccaaagtc gctcatggtg ccgtcttttt ccatccccgg agtcagcagg
    16501 gtgactttga tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa gccgtgccag
    16561 gtgctgtcag aacgcagcgg ccagcattca gtggtatcga tatgatccgg ctgccataca
    16621 tcaaagaacc agccatcaga ttccgttctc agacgtttga tctctttacg gaatttgatc
    16681 gcacgttcaa tggaaccgtt gatcagacgc ttaccagcat tgcctttcat catcgccgct
    16741 gcggtttcag tggacgccac gataccgtag tgcggagaag tggtggtgtg catcatgtag
    16801 gcttcgttaa aggtttcttc gtttacgtca cctttaacgt ggatcatgga agcctgagag
    16861 aacgccgcca gcagtttgtg agtggactgg gtttcgtaaa tcactttccc ttctacacgg
    16921 ccaccgctca taccgcattt accttcgtaa atcggtgaga agttggtgta aggcacccac
    16981 gcggagtcaa agtggatgga tttcacatcc agtgttttct tgataaagtc ggtgttgtac
    17041 agcagaccat cataggtaga gttggtaatt acagcatgta ccggccaggt tgcgtttggt
    17101 gtttctttca cgcgcttagc aatggtagcg tgctggaatt cactctgtgg gataccacca
    17161 agaataccgt aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc
    17221 atcaggtggg tcagcgattt gtggcagtta cggtcaatca gaatggtgct gcctgctgga
    17281 gcagagtaca taccaacaat tttgttcgca gtggaagtac cgttggtcac catgtagctg
    17341 cggtctgcgt taaagacgcg agcgatatac tgttctgctt ctttgtgtgg accactgtga
    17401 tccagcagag aacccagttc agatactgaa atggaaatat cagatttcat ggtgttcgga
    17461 ccaaagaaat catagaacag gctacctacc gggcttttct ggaatgcagt accgcccatg
    17521 tgaccaggag tacagaaagt atatttacct tcacgaacat atttaaacag tgctttagtc
    17581 agcggaggca gaatagtgtt gatatattcg tcagtggtct gcttgatctt attagcaata
    17641 tcttcagcag cacccagcgc atattcaaag aagctaatct gtaaacgcag gtcattcagg
    17701 cttacatcga gagtggaata cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt
    17761 ttgctaattt cttcgcacag ctcgagatta tatttatccc agtcaaaaat aacgccgcac
    17821 agacgcgcat tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc
    17881 tggaagttca gacgttcaag cgcgcgatga agttcacgga tgggttcttc tttaaaataa
    17941 acccccatgt gattcaatat tgcaataacg ttcatagtca tatctccagg taaaaaaggc
    18001 ccctcccaac acatgggaca aaatgaaagg aggagcctcg gaaaatactt ttaattaatg
    18061 tgcgttagac gcggtgtggt tatccattga gtggctctgg cgctcgtgca ttttgcgagc
    18121 gtagaacatc aggataatca ggctgacgat gaaggtacct gccagctcga aggagcttgc
    18181 gcccatcagc gcgatgaagc agaacacgca acccagtaca gagcagatca ggctgacaaa
    18241 gttgcggatg ttaacgcctt caaaacgaat caggtcaacg caagagtaga agtacggcag
    18301 catagtcagc agtactgcga taccggtcag ttcaccgaac aggtcagatg ctttaccacc
    18361 ggcagagttc atcagagtga tgaggatcat cagggcagtc attttcactg cagccagcag
    18421 cagacctttt ttcggaatac cgttgctgtc gacttcacca taaactttcg ggaagttacc
    18481 gtcgttagcg gcacgtacac ctgcctggcc taccaacatc atccaggagc ccagagaagt
    18541 caggcacgca aaggcggtga atgcagaaac cagcggcgca gcccagttac cgaggatggt
    18601 tgaagcactg attgcaaacg gagcaccgga agctgccatt acagaagatg gatacatacc
    18661 ggaaagcacc tgagtcgcag cgatgtaaac aatacctgct aaaccagtac ccagcatggt
    18721 tgccagcgga acggtacgtt tcgggttttt aaccatacca gtacttactg ctgcggattc
    18781 aacacccacg aaggcccaca ggcagagcag aatactttta atgatcgcat gaccatcagt
    18841 ggtatccgca gtattccagt tagctgcata agttgccgca tcaaaccaat gccagccaac
    18901 aatagcagtc atcaccacag gaataagaac cagcaccaga ccaatagtgg ttaaacggct
    18961 tacccaggta ccgccgagca tatttacaaa ggtaaatacc cagacgatag caatacaggc
    19021 gatacccgcc ggaacaggat catttaatac tgggaagaag gtggaaagat aagatacagc
    19081 ggtaatacca atcgccaggt taccaatcca gttagcatgg taataaagaa cacctgtctg
    19141 aaaaccaaat gcaggggaaa tttctccggc ataagcaatt gggccacctt gttgcgggtt
    19201 ttttgttgcc agtcgggcat atacatacgc cagcgacatt gcaccaataa tagagataat
    19261 ccaaccccag atagcaatac caccgatact tgctaggttc gcaggtaata atgcaatacc
    19321 gctccccatc atattaccgg caacaacacc ggtacaggca aatagcccga tcttcttggc
    19381 agaactcatg ctcttctcct aatttcattt ttgaatttgg agtccgggtc atgatgtata
    19441 actatttcct gaccagacca aactggcgct aagaatactc acgaaaaaag gattaatcct
    19501 aaagattagg tgaaataaca caaaagtttc tgtaagtgag aacttgaggt tttttattaa
    19561 cacatcagga tcgcaagttg atatcatgaa aagataaaca ttcaatgttt acaatggatt
    19621 gcatgacatt ctctggttaa atttatgtaa taaaaattat gcggcaaata aattgccgca
    19681 acatattata ccaacgggaa catacaaaaa ctcaacaaca aatattccag agcataaatc
    19741 aacccggagt tacttattct gaagcgagaa atttgtcgag ataaggtaca acataaggaa
    19801 cagaagtctg gaatatacca ttttcaatcc agtaaagggt gtttgcccct gggcgtaaat
    19861 taaaggcggt gagatatgca tcagctgctt cccggttcat ccccttcatt tcataaacct
    19921 tgccaagcaa tacataattt agccaggaca tttcaagatc aatgccagta tttatcgcct
    19981 ggtaagactc atctgtttta ccttttacca gagcactgac cgcttttatt tgatatataa
    20041 tggacaggtt attcaattcc ggtagtgtaa caatgttatc tatttctgtg ttcagtgctg
    20101 ctaattgttt ttcatctaaa ggatgttgag aatggcgcac gatatcaact aatgcttttt
    20161 ctgctctcgc gtaggtaaat tctggggatg attgaacaat ctcacctaat aattcactgg
    20221 cacggttcaa tgatttatca tcgccatgca gtaaataatc atgtgcctga taaaaattag
    20281 ttaataacgc accacgatga ggcaaaattt gctggagcgt ctcctgcatt cgttgtggcc
    20341 acggttggtt taacgctttt gataaactct ccagtaaatc attttgaatc gccagctgat
    20401 taccgttagt gatgacataa cgtttatcca gcatggttga accatctgca ttgtctacca
    20461 attttatcga cataaagcat tgttgagcac ggtattggcg ctgattaaca aacgcaatag
    20521 ataatgtttt accggaactg ctcggttcat caatgttgta gttgattttg tcatgcacca
    20581 taaaggtgga gaaggtgtta agtgatgtcg ccaccaaatc acccacgcct atcgcgtaag
    20641 agagctgata cggggaactc cagctgttac aacttttatt taccatatta atgtcaatat
    20701 cgcgtggatt gagcaaaata cgcgatttgc tcataggaag ccgtgtatca agagttgaaa
    20761 acgctaccag tgctacacag atacctaacg acaacaggaa aaaaaaccat acccaaaagg
    20821 tagtgaatcg tttgctttta actggggatt gttcaggtgg cgttgcggtg ttttgaatgt
    20881 taagactgtg ggagggagaa tctgtggcag gaaccgcctc tggtataggg ggaggcgaag
    20941 atagcattat ttcctctccc tcttcttcgc tgtaccagat aaccggcacc attaatttat
    21001 agccgcgctt tggtacagta gcgatataga caggactatc ttcatcatta tcttttaatg
    21061 acttacgtag ttctgagata ctctgcgtca caacatgatt ggtgacaata cttctcttcc
    21121 agacattatc gataagttca tccctgctaa gtacttcgcc actgtgttga gcaaagaaaa
    21181 ccagaagatc gattaatctc ggctcaaggg taagttgacg cccattgcgg ctaatttggt
    21241 ttatggacgg agtaacaagc cattcgccaa cgcgaactac aggttgttgc ataatagaaa
    21301 ctcattcgaa aagggaatga tgcaatgata attgccacaa cctattttta ccatctatag
    21361 atgggtttat ttacatatta ttggtgaatg caagacgtta tttttaccag ccataaactt
    21421 cttgattaca tagtattacg aaaggatttt actgagaacc agaagtaata ttccttacca
    21481 tcaaaattca tcatctttgc caaagaaaaa tgttcagaaa ataatccatg gaaaattgtc
    21541 cggagcgctt actattttaa tggattgtta gtctttgcat gagcaagcgg actgatacat
    21601 ttctctttgt tctcattcag aaaatctcat cagtcggcgt tcagaccggt gttgtgcttt
    21661 atggatgcgt tacgctcctg atgacgtcat ttgacgttca acaacatcac ggggccgcac
    21721 gacatttcac gtcagttagt gctatcgctc aggaacaaat tttcccgaat tgggatatgc
    21781 ccgcaaattg ctggtgatga ggggagaatc tggttgagtt cgggagaatt gatttggagg
    21841 cagaacgctt aaatcgtggc gtcctgaaac gaaaaacgga cctccgtgga ggtccgttta
    21901 tatgaatttg gtgccggact cggaatcgaa gatttcattt atcatattga tattaatctc
    21961 gtttatttgg tttgatgaaa aatgtaccct ctcttgtacc ctcggtccct tctggtggtt
    22021 tttatgagaa caagtagatc aagatttttg tcatcagaac acgtgtattt ttgtgggttt
    22081 tattcctgag ttttattcct ttttatatcg ctaacgctcc gggatagtgc ttagcaataa
    22141 tatcattaag cctgtcaacc agctctggtt tcctgaacgt gatgtgcgcg gagcccttct
    22201 taaagtatct gatgctgaac atctcatctt catagtcctc tttaccctga acagactgga
    22261 cgtgatcatc cagatgaata gtgatgtttt cccggttgtc ggggatcggt ttgccgctga
    22321 acaggtgtag cattatttcc agatcagcaa gtcgatctgc ctgttgcccg gtgataagat
    22381 gaaagcccca ccggtcccac ctcaccaggt tgttgacgat aattttactg ccaaatttgc
    22441 aggggcaatt ggttttgtaa ttccagctca accctctgaa gacgttgatc accccccgct
    22501 caaacacctc atccttattc tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcac
    22561 tgatttccgg aaaattatcg tattccagtg accggtacca tgtatcacgg gcctgtgcat
    22621 ccattaagga aagcatcccg gaacgttgca tcaggtcacg ccagatttcg cgatcgagat
    22681 tgcgtgtgat ggctttcatt gcggtttcag gtttctccat cagccagcaa ccgcagtgaa
    22741 aattctgttt catggcccag tccagtgcgg ttttaccgcc aatgctttct gtcattgctg
    22801 agatttcagc cagttgccgg ataagtgtgt cgatttgcag cagtgtactc tttcgtacag
    22861 ccagaatgtg gcggatactg gttgagcaaa taacgtccgt atgcccggtg accacttccg
    22921 gacgggagac tgtgattgca gacataatcg atattccttt tcgcagataa atgaaaacgc
    22981 cagccctgtt caggctggcg tcagtggaat gaagtatggt gagtgagact caccggtttt
    23041 gttgcagaaa ctgcagttgg cgcaggatac ggatatcttc cctggcctgt tcaatacagg
    23101 gcaacagtgc tgccgaatcc gttttatcct catcgttgtc gaagataatc cctgattcgc
    23161 agtcaatatt gtcctgcagc cacgcaatca gaatatccag cgccgtttct gtggttaata
    23221 atttcatcca gtgtcttttg tcctgattac cagtcgaaag tgggtaaacc cggcaggcat
    23281 tcagcgctgg catccagatg aatgagactg acgccataac gtcgggtaag tgtgacaacc
    23341 agacggcgga acgtttttga cagccccagg cgtttaagcc gcagaaccgg gtatgaccac
    23401 gcatcggtac gtaacagata tccgctacct gtgaaatgaa tccattctga ttcgccggtg
    23461 tcattcgtct gatgtgacag cgagtacagc caggcgttgt cctttgcagt gatatgcgcg
    23521 gtactgcagc gtatgccggt cagggcagca aatggtggcg tgcagtctgt tgagacccgg
    23581 tcagattcat caacaacacg aagggagtgg ccattgtcgc agaccacgtt aatcagttca
    23641 gcgagattaa caccatcgac gtcaacgaca attcgtccca tgttcagcgc ctgcacgtta
    23701 acgctgtctg cttccagcgt cagagtcagt ttcattgttt cgcctccgga tgcttcccct
    23761 gggtaatgtt atttaccgtt ctgtaattgt cgcgggtcat caggccggtc gccctgcgag
    23821 cccggaggat atcgatgctg tttattaact gagaacgggt acaggcactg aattccggct
    23881 ggtcggtacg caccagtgcg tatttttcaa caagaaagtt cactgcatcg cacagtgaaa
    23941 tgccagcttc aatatgctgc tcaatcacac gttcatcggc aaacggtgtg tcattcagtg
    24001 tgaggccata atgctggtcc agcagtcgga tgagcagtgt ctgccagatg gtgacgggag
    24061 acgggcagcg agatacctcc cgtacatgtg tgtcggataa tgttttcatc ttaagttctc
    24121 tgtaactggt agtgctaaag gtgagagctt cggggttatg aggtggttgc gggtgcctgt
    24181 gccggataaa cagccagata aacgtaacca caggagccga gggtgtcggc ttcgcaggtc
    24241 agcccttttg catacagcgt gacggtatgc tgatttcggg gattcagttc accgctggtg
    24301 agcatgagct ccagttgttt catcagcagc ggaaaggcct ggtccagatg gtatgcatct
    24361 gcgtcgctga agcggcctct gataccggcg cggtcggcaa ggtaatgcag gcggttaccc
    24421 tcctgcacca gacgggcacc aaaacagggc ctcactgtgc agggaagccc ccaccagggg
    24481 cggtcgtgat tgtcgtcggg atgagttatc ccggagagtt tgtctgacac gataaaatcc
    24541 tctctgaaaa gtggttaaga atggtttaac gatgacaggt cagccggcgc gcagaccgcc
    24601 ttcggtaaag gtgtattcgt ttatgagcag ggcctcatcc acagcggcgt ctgagctcag
    24661 ccagtcgtac tcgttttccg ggtgacggaa catcagttca gggtgacgct catcagccgg
    24721 acatacgggc caaaactgtc cttatggcgt tcagcaaaca cggccagcac accgggaata
    24781 tcctgcactt cacgaccggt atacgcttca gt
//
LOCUS       DAZGCQ010000075        23971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000075 DAZGCQ010000000
VERSION     DAZGCQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.075"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            296..1501
                     /gene="gltS"
                     /locus_tag="ACHK07_003329"
     CDS             296..1501
                     /gene="gltS"
                     /locus_tag="ACHK07_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO8103618.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(1677..3758)
                     /gene="recG"
                     /locus_tag="ACHK07_003330"
     CDS             complement(1677..3758)
                     /gene="recG"
                     /locus_tag="ACHK07_003330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO8103619.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(3764..4453)
                     /gene="trmH"
                     /locus_tag="ACHK07_003331"
     CDS             complement(3764..4453)
                     /gene="trmH"
                     /locus_tag="ACHK07_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO8103620.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4460..6568)
                     /gene="spoT"
                     /locus_tag="ACHK07_003332"
     CDS             complement(4460..6568)
                     /gene="spoT"
                     /locus_tag="ACHK07_003332"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO8103621.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(6587..6862)
                     /gene="rpoZ"
                     /locus_tag="ACHK07_003333"
     CDS             complement(6587..6862)
                     /gene="rpoZ"
                     /locus_tag="ACHK07_003333"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO8103622.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(6917..7540)
                     /gene="gmk"
                     /locus_tag="ACHK07_003334"
     CDS             complement(6917..7540)
                     /gene="gmk"
                     /locus_tag="ACHK07_003334"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO8103623.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7798..9480
                     /gene="ligB"
                     /locus_tag="ACHK07_003335"
     CDS             7798..9480
                     /gene="ligB"
                     /locus_tag="ACHK07_003335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO8103624.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVADVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNQARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLVSTEQFWQQLPGIGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9477..10094)
                     /gene="yicG"
                     /locus_tag="ACHK07_003336"
     CDS             complement(9477..10094)
                     /gene="yicG"
                     /locus_tag="ACHK07_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO8103625.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10386..11210)
                     /gene="dinD"
                     /locus_tag="ACHK07_003337"
     CDS             complement(10386..11210)
                     /gene="dinD"
                     /locus_tag="ACHK07_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO8103626.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENCVKITEKK"
     gene            complement(11436..12293)
                     /gene="yicC"
                     /locus_tag="ACHK07_003338"
     CDS             complement(11436..12293)
                     /gene="yicC"
                     /locus_tag="ACHK07_003338"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO8103627.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQH"
     gene            12420..13136
                     /gene="rph"
                     /locus_tag="ACHK07_003339"
     CDS             12420..13136
                     /gene="rph"
                     /locus_tag="ACHK07_003339"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO8103628.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13202..13843
                     /gene="pyrE"
                     /locus_tag="ACHK07_003340"
     CDS             13202..13843
                     /gene="pyrE"
                     /locus_tag="ACHK07_003340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO8103629.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(13880..14476)
                     /gene="slmA"
                     /locus_tag="ACHK07_003341"
     CDS             complement(13880..14476)
                     /gene="slmA"
                     /locus_tag="ACHK07_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO8103630.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(14583..15041)
                     /gene="dut"
                     /locus_tag="ACHK07_003342"
     CDS             complement(14583..15041)
                     /gene="dut"
                     /locus_tag="ACHK07_003342"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO8103631.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15019..16239)
                     /gene="coaBC"
                     /locus_tag="ACHK07_003343"
     CDS             complement(15019..16239)
                     /gene="coaBC"
                     /locus_tag="ACHK07_003343"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO8103632.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16411..17079
                     /gene="radC"
                     /locus_tag="ACHK07_003344"
     CDS             16411..17079
                     /gene="radC"
                     /locus_tag="ACHK07_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8103633.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17296..17532
                     /gene="rpmB"
                     /locus_tag="ACHK07_003345"
     CDS             17296..17532
                     /gene="rpmB"
                     /locus_tag="ACHK07_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO8103634.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            17553..17720
                     /gene="rpmG"
                     /locus_tag="ACHK07_003346"
     CDS             17553..17720
                     /gene="rpmG"
                     /locus_tag="ACHK07_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO8103635.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            17818..18627
                     /gene="mutM"
                     /locus_tag="ACHK07_003347"
     CDS             17818..18627
                     /gene="mutM"
                     /locus_tag="ACHK07_003347"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO8103636.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18666..19145)
                     /gene="coaD"
                     /locus_tag="ACHK07_003348"
     CDS             complement(18666..19145)
                     /gene="coaD"
                     /locus_tag="ACHK07_003348"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO8103637.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19153..20430)
                     /gene="waaA"
                     /locus_tag="ACHK07_003349"
     CDS             complement(19153..20430)
                     /gene="waaA"
                     /locus_tag="ACHK07_003349"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO8103638.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            20843..21901
                     /gene="rfaQ"
                     /locus_tag="ACHK07_003350"
     CDS             20843..21901
                     /gene="rfaQ"
                     /locus_tag="ACHK07_003350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO8103639.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTSLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            21898..23022
                     /gene="waaG"
                     /locus_tag="ACHK07_003351"
     CDS             21898..23022
                     /gene="waaG"
                     /locus_tag="ACHK07_003351"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8103640.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23015..23812
                     /gene="rfaP"
                     /locus_tag="ACHK07_003352"
     CDS             23015..23812
                     /gene="rfaP"
                     /locus_tag="ACHK07_003352"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO8103641.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPSDVRVKRMLIKRVATMVRNMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5687 a         5925 c         6397 g         5962 t
ORIGIN      
        1 agggtggaaa cagacatcga aaacaatccc gtggtagcaa agcgggcatt ttagctgacc
       61 gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat atttaaatga gataaatgca
      121 cttttttata taacttttgg tatttttctg cttaaaatcc atgccatttg ttcattaaca
      181 gctgagaatt tgacccagga aagcataact cgatacccgt catagttcat aagttgccac
      241 tgcgcgatga agtatgacga gtatgaaaga gtgatgcgga tacaaaggag taactatgtt
      301 tcatctcgat actttagcaa cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa
      361 gttggtccat tccgtctcct ttttgaagaa atacaccata ccggaacctg ttgcgggtgg
      421 tttgttggtg gcgctggcgc tactggtact gaaaaaaagc atgggctggg aagtcaactt
      481 tgatatgtcc ctgcgcgatc cgttaatgct ggcattcttc gccaccattg gcctgaacgc
      541 caacattgcc agtttgcgtg ccggtgggcg tgtggttggc atcttcctga ttgtggttgt
      601 tggtctgttg gtgatgcaaa atgccattgg cattgggatg gctagcctgt tagggcttga
      661 tccgttgatg gggctgttgg ccggttctat tacgctttcc ggcggtcacg gtacgggagc
      721 tgcatggagt aaattgttca ttgaacgtta tggcttcacc aatgcgacag aagtggcgat
      781 ggcctgtgca acgttcggtc tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta
      841 tctggtgaaa cactccacca cgccgaacgg tattccggat gaccaggaag tcccgacggc
      901 gtttgaaaag ccggatgtgg gccgcatgat cacctcgctg gtgctgattg aaactatcgc
      961 gctgattgct atctgcctga cggtggggaa aattgttgcg caacttttgg ctggcactgc
     1021 ttttgaactg ccgaccttcg tctgtgtact gtttgttggc gtgattctga gcaacggtct
     1081 gtcaatgatg ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag
     1141 cctgtcgttg ttcctggcga tggcgctgat ggggctgaaa ctgtgggagc tggcttcgct
     1201 ggcgctgccg atgctggcga ttctggtggt acagaccatc ttcatggcgt tgtatgccat
     1261 cttcgttacc tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggcca
     1321 ctgtggtttt ggcctcggtg caacgccaac ggcaatcgcc aacatgcagg cgatcactga
     1381 acgttttggc ccgtcgcaca tggcgtttct ggtggtgccg atggtcggtg cgttctttat
     1441 cgatatcgtc aatgcgctgg tgattaagct gtatttgatg ttgccgattt ttgcgggtta
     1501 accgatgaag cggcggtaga agtgccgccg caacaaagac aaatgcctga tacgcttcgc
     1561 ttatcaaggc tgcgcattga ttcacaattt attgaattta taagtttttg taggacgaag
     1621 cacatccggc aggaaggtag ggtaacctga aatggcggtc ttctcactgc cgccttttac
     1681 gcattcgagt aacgttccgt ctccggcatc cagcgttcta tcagggcttt tgcctgttgt
     1741 gggtaacgtt cgtgaatatg gcgtgccagg cgctgaactt ccgggatcat cgcctgatcg
     1801 cgcagtaaat ccgccacttt aaattcagca ttacccgtct gacgagtgcc taacaattcg
     1861 ccagggccgc gaatctccag atctttttgt gcaatcacaa acccgtcgtt gctgtcgcgc
     1921 agcacttgca ggcgaatttg cgccgtttta gaaagcggcg ttttatagag cagcacgcag
     1981 tgagaagcca ccgcgccacg acctacacgc ccacgtagct ggtgcaactg cgccagaccc
     2041 agacgctccg ggttttcgat aatcatcaga ctggcgttag gcacatcaac gccgacttca
     2101 ataacggttg tggcaaccag caggtgtagc tcaccttgtt taaacgacgc catcaccgcc
     2161 tgtttctcgg caggtttcat ccgcccgtgt accaggccaa cattcaactc tggtagcgcc
     2221 agtttcaact cttcccaggt agcttccgcc gcctgcgctt ccagcaattc cgactcttca
     2281 atcaacgtac aaacccagta cgcctgacga ccttcagtca tgcaggcgtg gcgcacgcgg
     2341 tcaatgatgt cggtacggcg agtatcagga atagcgaccg tagtcactgg cgtgcggcct
     2401 ggcggcagtt catcaatgac cgaggtgtcg agatcggcat acgcagtcat cgccagcgtg
     2461 cgggggatcg gcgtggcggt catgatcaac tgatgcggat ggaagccttg ctgctggcct
     2521 ttctcccaca atgccagacg ctgatgcacg ccaaaacgat gctgttcgtc gataatcacc
     2581 agcgccagac cgttaaactg cacctgttcc tggaagatgg cgtgcgtacc aacaatcatc
     2641 tgcacctgac cgctggcgat ggcttcctgc tgagacagtc gcgctttacc tttctgctta
     2701 ccggcgagcc agcccacttc gataccgagc ggtgcaaacc agttgcggaa gttattggcg
     2761 tgctgttcgg cgagtaattc ggttggtgcc atcaatgcca cctgtttgcc gtgggcaatc
     2821 gcacgcagcg cggcgagggc agcgaccagc gttttaccgg aacctacatc gccctgtacc
     2881 aggcgcatca tcggcacatt cagcgccata tcgcgctcga tctccgccac cacgcgagcc
     2941 tgagcgcccg ttggcttgaa cggtaaggcg gcaagaagtt tatttttcag cgtgtcattg
     3001 gcgctcagcg gctgggcatg gaaacgttgc gctccggcgc gtaaggctaa catgctgagg
     3061 ttatgcgcca gcagttcttc cagaatcaga cgacgttgcg ccggatgctg cccggtttcc
     3121 agatcgctaa gttgcagcgt cggcggcggg cggtgcaaag tgcgcaacgc ttccggtagc
     3181 gtcatcattc cttgtgacag ttccggcggc aggagttctt caatggcgca ggtgtcgagc
     3241 agatccagcg cctggtcggt taatttacgc agcgtggcct gctttacgcc ttcggttgtt
     3301 ggataaaccg gcgtgagcgt ttcctgcaac tctggcgtac tgagatcgcc ctgcacgcgg
     3361 tattccgggt ggatcatctc cgcaccatat ttaccgcgct ttgcttctcc ataggccaac
     3421 acgcggcggc ccgtcgccag gctatttttc attgccgcgt tgaagttgaa aaagcgcatg
     3481 gtgagaatgc cggaaccgtc gctgatctgg caggtcatca tccgccgacc gccgaaggag
     3541 atattgcagt tcagcacttc gccttccacc gtggcataaa cgcccggtag tagttctccg
     3601 atggggtaga gatgggtgcg atcttcgtag cgcaggggaa ggtgtaagag tagatcctgc
     3661 acagtctgca ggttgatttt cgccagcttg ttactaagcg ctgcgccaac gcccgttagg
     3721 gaactgagcg ggacagcatc taacaggcga cctttcatgg cacttaccct gcagcctgca
     3781 tggtagccca ccagtcggca tcagcttcga tctcgccttg ctgattgacg tgggggtaag
     3841 gcaggccttt gcgttttgcg actttcgcca gcaccggata gccgccttca aacaacaggc
     3901 gttgttgctc tgcttccggc aacatgctgt tttcacgcag gtacatgcct gcattttgcc
     3961 gctgacgctg ggcttcgtaa agaatgaggg ctgaggcaac ggaaacattc agcgactgca
     4021 ccatgccgat catcggaatg atgatgtcct gatctgccag ggccaacgct tcctcagtga
     4081 tgcccgtttt ctcctgaccc atcaaaatgc aggtcgggcg agtgtaatca atttcgcgaa
     4141 aatcgacagc gttatcagaa agatgggttg ccagaatttg catgccctgg cctttgagat
     4201 gagcgacggc atcgccaatg gtgcggtgcg ttttcacctg cacccaactg ttgctacccg
     4261 ccgccgcaga ggccatggta cgcatgcggc taccaggcca gacggcgtga acttcatgta
     4321 cgccaacggc atctgcggta cgaataatcg cagaaacgtt atgaggtttg tggacctgct
     4381 ccatgcagac ggtcagatca ggctgccgcc tggcgagcat ttcgcagatg cgtgcataac
     4441 gtgttgggtt cataaaacat taatttcggt ttcgggtgac tttaatcacg tctggcatca
     4501 cgcggatttt gcgcatgata ttcgccagat gcacacggtc acgagcggtc agacgaataa
     4561 aggcgctgta gacgcgacca tctttctctt ccgtattcaa actttgaata ttcgaagtcg
     4621 tggtgttaat tgccgccgtc aggtttgcca gcgcgccctg atgattgaac atctccacct
     4681 tgatttcggt gatgaactcc tgcgccgtct ctttatccca ttccacagcc ataaacttct
     4741 ctggctcttt ctggtagcca cggatgttac ggcaggattc atggtggatc accagacctt
     4801 taccggggct gacgtgggcg ataatcgggt cgccaggaat agggcggcag catttcgcaa
     4861 aggtgatcag cacgccatcg gcacctttaa tgggcagatg tccgtgactt tgggttgccg
     4921 gtggaatgga ggcgtcccca tgttgcagat ttttcgcgac caccacgctc attgcgttac
     4981 caagtccgat ttctgccagc agatcgtcaa gcgttgccag cttcatacga tccagctcgc
     5041 gctgaatatt ttcctgcggg atttcattca gcttacggct accacccaaa gcatggttga
     5101 gcagacgacg gcccaggctt acagaatcat cacgcttgag gtttttcagc aactgacgaa
     5161 ttttggcgcg cgctttcgag ctaacgacaa agttcagcca cgcggcattc gggcgagcgc
     5221 ccggtgcggt aatgatttca acggtttgac cgctagtaag cggctgcgac agcgggtaag
     5281 gctggcggtc gacgcgtgcg cccacgcagg catgaccgat atcggtatgc actgcataag
     5341 cgaagtcgac gggcgttgca ccggcaggca gttcgacaat gcgcccttcc ggtgtgaaaa
     5401 cgtaaatctc atccgggaag agatcggatt taacgctctc gataaattca aacgagctac
     5461 cggcgctttg ttgcagctcc agcaggcttt gcatccagcg ctgggcgcgg atttgtgcag
     5521 tcgtgctggt ttcgccgtgc tctttataag cccagtgcgc ggcaacaccc atctccgcca
     5581 tctgatccat atcttcggta cggatttgga cctcaaccgg cacgccgtgc gggccaatca
     5641 tcgaggtgtg caacgactga tagccgttcg cttttggaat agcgatgtag tctttcacgc
     5701 gacccggacg cggcttatac aggctatgca tctggccgag cacgcgataa caggtgtcgg
     5761 aatcattgac gatcacgcgg aaagcgtaga tgtccatgat cgagtgaaaa cgctgctctt
     5821 tgagcaccat tttgcagtaa atcgaataaa gatgcttctc gcgaccactg acgcggcacg
     5881 gtattcccgc ttcctgcaaa cgcccttcga tttcagagag gattttttgg atcatctctt
     5941 tacggttgcc gcgcgcggct ttcaccactt ctttaattac gcggtaacga ttgggataca
     6001 gcgcctcaaa acccagctct tcgagttcgg ttttaatgtg gtggatacct aaacggtgcg
     6061 ccagagggct ataaatttcg agagtttcac gggcgatgcg gcgacgtttg tccgggcgaa
     6121 gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag tttgatgaga atgacgcgga
     6181 tatcctgcac catcgccata atcatcttgc gaaagttttc ggcctgcgcc tctttcttat
     6241 cgcggaactt gagtttatca agtttcgaca ccccctctac cagctcggcg acgcttttac
     6301 caaaaagctg ttccatatcc tggtaggtgg cgggagtatc ttcaatcacg tcatgcagca
     6361 gcgctgccat cagggtttca tagtcgagtt tcatctcggc cagaatgcag gcaacagcta
     6421 ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg cccctcgtga gcatcacgtg
     6481 caacgagata cgcctgccgc agacgcttga tttggtcttc cggcaggtag ttttgaatca
     6541 gctgattcag gctttcaaac agatacaagg gcgacccgct ttgtgattaa cgacgacctt
     6601 cagcaatagc ggtaacggct tgtaattcag cggcttcctg ctcttgctgt tcctggcgtt
     6661 cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc gatttcgcgc agcgcgatta
     6721 cagtggtttt atcgttttct tccggtacca gcggatcctt tccgcctacc tgcatctgac
     6781 gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt accaattttc tctacagcgt
     6841 cctgaacagt tacgcgtgcc atacttaaaa agctccacag gtgaagaaat gactgggcat
     6901 gatactgaaa tcaggttcag tctgccaaca atttgctgat taaagcgtca tgacgctgct
     6961 tttggcggct catgcgcaga cgttcggcgc gaataatggt cttcaaatcg gtcaacgcgg
     7021 tatcgaagtc atcattcaca atcagataat catattcggc gtaatggctc atttctgcaa
     7081 cagcttgcgc catacgcttt gcaatgacct cttcgctgtc ctgaccgcga ccgcgtagac
     7141 ggcggtccag ttcaattttg gacggcggta aaataaagat actccgcgcg tgcggcatct
     7201 tctggcgaat ttgctgcgca ccctgccagt cgatatcgag aaaaacatcg acaccggtcg
     7261 ccagtacttg ctcaatggcc tcacgcgaag tgccatagta attaccaaaa acttctgcgt
     7321 gttcgaggaa cgcatctctg ctaatcattt ctttaaattc atcatgatta acaaagaaat
     7381 aatgttcacc gtggacttca ccaggacgcg gttggcgtgt ggtgtgtgaa acagaaactt
     7441 gggtgtcata caacggttgg gtttttaata aagcctgaat caggctggat ttacccgcgc
     7501 cactgggggc agaaacaata taaagcgtgc cttgagccat gagtatcttt cgtatgtgat
     7561 tagcgaaata aagcctacat acgagcttat tatacacggc gcggccatgt gacgtagccc
     7621 ttgctcacac tttttgcgca aagttgttgc gttttgcgta gctgcttccg catttgccag
     7681 tgattcactg caataaagac gattctcagg aagcgtcctc gcagactccg tttttgccac
     7741 tcggctttgc tttcatcacg ttgtatatct gcctgactat ctacagcgga ggtgatgatg
     7801 aaagtatgga tggcgatatt aataagtatc ttgtgctggc aatcatctgt gtgggcggtc
     7861 tgtccggcct ggtcgccagc cagagcacag gaagaaattt cccgcctgca acagcaaata
     7921 aaacagtggg acgatgacta ctggaaggaa ggaaaaagtg aggtggaaga cggtgtttac
     7981 gatcagttaa gcgcccgtct tacgcagtgg caacgctgct ttgggagcga gccccgcgac
     8041 gtgatgatgc cgcctttaaa tggtgcggtt atgcatccgg ttgcccatac gggtgtacgc
     8101 aaaatggtgg ataaaaacgc attaagtctg tggatgcgag aacgtagcga tctttgggtg
     8161 cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa actgaacaaa
     8221 gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga cgcagaaagt tagcttaatt
     8281 tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gtacgcttca gggggaaata
     8341 tttctccagc gcgaggggca tatccaacaa caaatggggg gaataaatgc ccgcgcaaaa
     8401 gttgctggcc tgatgatgcg ccaggggaat agcgacacgc taaactcact ggctgttttt
     8461 gtctgggcat ggccggatgg accgcagtta atgtccgatc gtttaaaaga gctggctacc
     8521 gctggtttta ctctaacgca gacgtatacc cgtgcggtga aaaatgctga tgaagttgcg
     8581 cgcgtacgca atgagtggtg gaaagcgaaa ttacccttcg tcaccgatgg cgtagttgta
     8641 cgagcggcga aagagccaga atcccgccat tggctaccgg gccaggcaga gtggctggta
     8701 gcctggaaat atcaacctgt agctcaggtt gccgatgtga aggcgattca gtttgcggtg
     8761 ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac ctgtcatgct ggatgataaa
     8821 aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga tattgcacct
     8881 ggtgatcaga tcctcgtcag ccttgccggt cagggtattc ctcgcattga tgatgtggtc
     8941 tggcgcggtg cagaacgtac aaaaccgaca ccaccagaaa accgctttaa ctcgttgacc
     9001 tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt ctggctgggg
     9061 tcaaaacagg ttctggggct ggatggcatt ggtgaggccg gttggcgcgc gctgcatcag
     9121 actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca attacagaac
     9181 acgccgggga tcgcgaaaag taaaagtgcg cagttatggc atcagtttaa tcaggctcgt
     9241 aagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg ggcggcgctt
     9301 aatgccagtg atgaacggtc ctggtcgcaa cttttagtta gcacggaaca gttctggcag
     9361 cagctgccgg ggattggatc tgggcgcgcc agacaggtta ttgaatggaa ggaaaatgcg
     9421 caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt tgaaccttag
     9481 tgaccttcgt ggctgtagta aaaaaccggt aatcccagct taagacgtag cgccagtaaa
     9541 cgggcaaaaa agccgaatac cagggtggag ataatcacca catcatggtt agaaacatag
     9601 tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata caactctttc
     9661 tggaatacca gtgggatgcg tttacagaac atatcgcgca aaacgccgcc aaacacgccg
     9721 gtggttaccg ccgcgacaac ggcaataatt gggccgtgcc ccatatccag tgcaacctgt
     9781 gcgccaatga tagaaaagac caccagtccg agcgcatcga gcaccagaaa cactttgcgc
     9841 aggtaaggca ttacaggagc aacgatagtg gtgagtactg cggcggtagc gacgataatc
     9901 acatattccg ggtgtttgac ccagccaagc ggatagtggc ccagcagaat atcgcgcact
     9961 gaccctccgc caattgcggt ggcggtcgca ataataatta cgccaaatgt atccatgcgc
    10021 cgtcgcccgg ccgccagcgc ccccgtcatg gcttcggcag taatgccaac caaatacaaa
    10081 atgtgtaaca gcatggttct ctccctaaaa atctgtcttg cagggtagcg atttgtgcgc
    10141 attgtcacga ttgagatttt ttaaggtgag ggggaattga taacttgtag ttatgtgaaa
    10201 tcgctatttt ctgtagcgga gatgcattct tctgacttct tgtctgagaa aatgcataag
    10261 aaaaaatagg ttaaaactga ggggtggaaa agaaaggtgc aatgtttagc tattgtattc
    10321 accgcacctg gactttggct tacgcatcgg gtgtctcaat caacaaaaag agtttttagt
    10381 ttctgctatt tcttctctgt aatttttaca cagttttcca actgcttgat gctgatctgc
    10441 ggggtcggca actcttcagg catggttccg ccaagttcct gaatggtttg tctcaccttg
    10501 cgacccacgt caaagtgtgt ggtgttagct tgctgttttg aattcacgcc atcccgcttg
    10561 agtttttctt ctgtttgggt agctcgaaag agattagccg ccagttctgt tgaacccata
    10621 tgatccagga tcttctgact ctttttcagc cccttccgct gatgaatagc tttctgatct
    10681 aatccaccat acagcccccg gtaaccatga ttctgaaata tggcgaaatc gatagctgtc
    10741 gctaccccag cttgctgcgc tgcctcaact aattgtttgt tatgttcttt caattcattt
    10801 ctcagaaaaa gacgcttttc gtcttcacga agttgcctga aagcttcatc gtcagccagc
    10861 tcctgccgtc gggtctggat agcaaaataa gtttgccctg ccgcaataac cggtttcgca
    10921 gggtcgccgt tttgtactac caggtaacag gcatagcggg agagatgaac atcttcaagt
    10981 tctcgttgag cccctgaacc taatacaacc attttggtgg tttcaacgaa atggtcagat
    11041 gcagcttgat tgctggcttc gcacgcttga gtcgcgcggg ccagtacctt ttgaaaattg
    11101 cgccagtccc gataatccag taacggtgcc aattctctcg ctgaccaaaa ttcttgccct
    11161 tcagtgccat aatgccgtat ttcttcaaaa ggttgatgat gttcgttcat tgcccactcc
    11221 atgtgatatc gattgcaaag aaagtaaatc tgtaactgta tttatataca gttgtgagta
    11281 tctcacgttc tcagtgtgct gggcaaaaag acatcgcaaa atctggaaca ggcacgcaaa
    11341 acagaattga aagttgcgga gaagaaaaat gccgctctgt ttgagtgggc ggcatttgtg
    11401 aaggctgaaa agtaagaatt ggcgttacga gttattcaat gttgaatctg ctcgcgcatc
    11461 tgctcaatca acactttcag ctcaatcgcg gagtttgtca cttcggcatt gatagacttc
    11521 gaagcaagag tgttcgactc gcggttgaac tcctgcatca taaagtccag acgacgacca
    11581 acggcttctt ttttcttcag aatgttgtac gtctctttaa catgcgcttc gaggcggtcc
    11641 agttcttcgg caacgtcaat tcgttgtgcc agcagaacca actcctgctc cagacggttg
    11701 ttttccaact gcacctgagc atcttccagc ttcgcgacca agcgttcacg ctgccattgc
    11761 aggatttccg gcatatggga gcggactttg accacttcgg cggtgacgcc ttccagacgc
    11821 tgctcaatca acgctttcag cgcctgacct tcggtttcgc gagcgatgat aaagtcatcc
    11881 agggtgccat ccaacgccgc gagaatttca gcggcaatcg cgtcgagatc ctgctcctgg
    11941 gccgccatca cgcccggcca acggagaata tcaactgggt tgatttctcc ttcgtcgctc
    12001 tgcattttta cccagttcgc ggcagttacc agctgtttag ccagtttttc gttgaggatc
    12061 agctcacctt gcgcgctaac atctggctca tagcgcaggg tacattccac tttaccgcgt
    12121 gtcaggcgag aacgaatacg ctcacgaacg acaggttcaa ggctacggaa ctgctccggc
    12181 agacgaaagt aagtttccag ataacgctgg tttaccgagc gcatttccca ggttgcgctc
    12241 ccccattcac ccttgatttc acgccgggcg taggcggtca tactgcggat catagacgtt
    12301 cctgtttata aaaggagagg tggaaggatt atagccatcg atgccttgtc aggataggaa
    12361 taaccgccgg aagtccgtat aatgcgcagc cacatttgtt tcaagccgga gatttcaata
    12421 tgcgtccagc aggccgtagc aataatcagg tgcgtcccgt taccctgact cgtaactata
    12481 caaaacatgc agaaggctcg gtgctggtcg aatttggcga taccaaagtg ttgtgtaccg
    12541 cctctattga agaaggcgtg ccgcgcttcc tgaaaggtca gggccagggc tggatcaccg
    12601 cagagtacgg catgctgcca cgttctaccc acacccgtaa cgctcgtgaa gcggcgaaag
    12661 gaaagcaggg tggacgtaca atggaaatcc agcgtctgat cgcccgtgcg cttcgcgcgg
    12721 cagtagattt gaaagcgctg ggtgagttca ccattacgct ggactgcgac gtgcttcagg
    12781 ctgatggtgg cacgcgtacc gcttcgatta cgggtgcctg cgtggcgctg gcagatgcgt
    12841 tacagaagct ggtggaaaac ggcaagctga aaaccaatcc aatgaaaggg atggtagccg
    12901 cggtttctgt cggtattgtt aacggcgaag caatttgcga tctggaatac gttgaagact
    12961 cagccgcaga gaccgacatg aacgtagtga tgaccgaaga cgggcgcatc attgaagtgc
    13021 aggggacggc agaaggcgag ccgttcaccc atgaagagtt actcaccttg ttggcgctgg
    13081 cccgaggggg aatcgaatcc attgtagcga cgcagaaggc ggcgctggca aactgatttt
    13141 taaggcgact gatgagtcgc cttttttttg tctgtagaaa agtaagatga ggagcgaagg
    13201 catgaaacca tatcagcgcc agtttattga atttgcgctt agcaagcagg tgttaaagtt
    13261 tggtgagttt acgctgaaat ccgggcgcaa aagcccctat ttcttcaacg ccgggctgtt
    13321 taataccggg cgcgatctgg cactgttagg ccgtttttac gctgaagcat tggtggattc
    13381 cggcattgag ttcgatctgc tgtttggccc tgcttacaaa gggatcccga ttgctaccac
    13441 aaccgccgtg gcgctggcgg agcatcatga cctggacctg ccgtactgct ttaaccgcaa
    13501 agaggcaaaa gaccacggtg aaggcggcaa tctggtcggt agcgcgttac aaggacgcgt
    13561 aatgctggtg gatgatgtga tcaccgccgg aacggcgatt cgcgagtcga tggaaattat
    13621 tcaggccaat ggcgcgacgc ttgctggcgt attgatttcg cttgatcgtc aggaacgcgg
    13681 gcgcggcgag atttcggcga ttcaggaagt tgagcgtgat tacaactgca aagtgatctc
    13741 tatcatcacc ctgaaagatc tgattgccta cctggaagag aagccggaaa tggcggaaca
    13801 tctggcggcg gttaaggcct atcgcgaaga gttcggcgtt taaagaaact cgccggatga
    13861 aaagtcatcc ggcgtcatat tactgcaact gcgcggcaat tagcggccag cgggcgtcaa
    13921 aatcatccgt cgggcgatat ttaaattcgc tgcggacaaa acgtgacagc ataccttcac
    13981 agaaggccag caactggctt gccagcagcg tttcatcagt ggtgtaacct tcaccctcac
    14041 gcattctctt ttcacgcaat acctgacgca gctgcgcttc aatacgctcg aaaagttggt
    14101 tgatgcgccc ttgcaggcgg tcttgttcaa acatcagcgc atgcccggta aggatgcggg
    14161 tcaggccagg attacgctca ccaaaaccga gaagcagcaa cactatcaga cgcaggcgcg
    14221 ctgtggtgtc tttctcatct ttcagaatca gattgatgcg agtaatcagg ctatcttcga
    14281 taaactcaat caggctatcg aacatgcggg tcttactggg gaagtggcga tacagtgccg
    14341 cttcggaaac gccgacagag gcggccagtt ttgccgtcgt gatacgttgg cttccatcgc
    14401 tggattccag catcagcgcc agagactgaa gtatttcctc gcgacggttc cttttcgcag
    14461 tttgtttttc tgccatgtta caaaataccc ctgaaaataa gcacttgcca ggcggcaccc
    14521 acgctatgac cgcaaacgaa atgtttgcgg ctatgttatg acgttattcg gatgcgtatg
    14581 tgttactgac gaccagagtg accaaagccg ccttcaccgc ggtcggtggc gtcgaaatct
    14641 tccaccagat taaattcagc ctgtactacc ggaacaaaaa tcatctgggc gatgcgttcg
    14701 ccaggttgaa tggtgaagct gtcctgacca cggttccaca cggaaatcat caactggccc
    14761 tgatagtcgg aatcgatcaa tcctaccagg ttaccaagca cgataccgtg cttatgtccc
    14821 aatccggagc gcggcagcat cattgccgcc agtgaaggat cggcaatatg aattgccagc
    14881 ccggtcggaa ccagcgtagt gtcacccgga gccagttcta cggcgtcgtc gagacaggca
    14941 cgcaggtcaa gtccggcaga gccagaggtg gcataagtcg ggagcggaaa ttccttccca
    15001 acgcgcgggt ccagaatctt aacgtcgatt tttttcatca taacgggtca cgatctcgtc
    15061 gagtaataat tggccaagga gctctttgcg ctcaagcggt aagactttat ctccgtcctg
    15121 ccagaaaagg tgtaatgcgt tgttgtcgct gttaaatcct tgagttggct gggaaacatc
    15181 gttcgcgcag atcagatcaa ggtttttacg gatacgtttt tgccgggcgt attcttccac
    15241 attatttgtt tcggcggcaa atccaacgac gtagggtcga tggtctttta gtgcggcaac
    15301 gcctgcaacg atatcggggt ttttaaccat ttttattgtt aattcatcac cctgcgtggc
    15361 ctgctttttg attttttctg gggccacggt agctgcgcga taatccgcca cggcggcgca
    15421 gccgataaaa atattttgct gctgtacaga agcattcacg gcggcttcca tttccagcgc
    15481 ggtcatcaca tcaacacgtt taacaaacgg tggcgtcggt agtgaaactg gacctgatac
    15541 cagcgtgacg ttcgcgccac gacgggcagc ggcggcggcg atagcaaaac ccatcttgcc
    15601 ggagctgtga ttagagatat aacgcaccgg atcgagcggt tcacgcgtcg ggccggcggt
    15661 aatcataatg ttcagatgtt tcaggtcgtt gacgggcgaa aaatgcgcta ctgccatatc
    15721 cacaatggtt aacggatcga gcattcgccc aggaccgata tcaccacaag cctgactgcc
    15781 actgtctggc ccccagatga gcaaaccacg ggaagcaagc acctctaaat tatgctgcgt
    15841 ggcagcggca cggtacatct gctggttcat ggcggggagc acggctacag gcgcaggtgt
    15901 agccagacaa atcgtcgata ccaggtcatt cgccattccg gcagcaacac gggcaatcaa
    15961 atctgccgtg gcgggggcga gaatcactaa atcagcccat ttacccagct caatatggcc
    16021 catagcggct tcggctgccg ggtcaagcag actgtcggaa acgggatacc cagaaaccgc
    16081 ctgcaagcta agtggggtga taaaggcttt tgccgcttcg gtcatggcta cgcggacgtc
    16141 ggccccgcga tcgcgcaaac gacgcaccag ttcaggggtt ttataggcag caataccgcc
    16201 gctaacgccg agaacgattt ttttaccggc caggctcatg atgatttttc ctgtagggtg
    16261 acaccagaag ttggcgattt tatcacaata cttttgttgt cgtgccttca cctgagattc
    16321 actttgcgag gcgctttcca ggattgaaaa ctgaccgtcg atttaacgga acggctatga
    16381 caggatgcga gcaccacaaa ggaggtgaag gtgaaaaaca atgcacagct gttgatgccg
    16441 cgcgaaaaaa tgttgaagtt tggtattagc gccttaacgg atgtcgagct gctggcgcta
    16501 tttctgcgta ccggaacgcg cggtaaagat gtattaaccc tggcaaaaga gatgctggag
    16561 aatttcggct ctctttatgg cttgttaacc tctgaatatg aacaatttag tggtgttcat
    16621 ggaattggcg tagcgaaatt tgcccagtta aaggggattg ctgaactggc gcggcgttac
    16681 tacaacgtgc ggatgcgtga agaaagccct ttactcagcc cggagatgac gcgggaattt
    16741 ttacaaagcc agctcacggg tgaggagcgg gagatcttta tggtgatctt tctcgactca
    16801 caacaccggg ttataacgca tagccgtctt ttttccggca cgctaaacca tgttgaagtc
    16861 catcctcggg aaattatccg cgaagcgata aaaataaacg cctcggcgct gatccttgca
    16921 cataatcacc cttcgggttg tgctgaaccc agtaaagcgg ataaactcat tactgaacgg
    16981 ataataaaga gttgtcagtt catggattta cgcgtgctcg accatatcgt gattgggcgt
    17041 ggagagtatg tttcttttgc cgaacgcggc tggatttaac ccgctatgcg cgatccttcg
    17101 ggatctttgt ctgttcggga cttgagcaca tcgctgagtc agcgtatact acgccacctt
    17161 tgagaatctc gggtttggca tttgggcctg gcaatcgaga gttcacatag aactgcgatg
    17221 accgggctgt aaagcctgac gaggcgccaa taccccatac gaagctcgag ctaatttgat
    17281 ttttggagaa tagacatgtc ccgagtctgc caagttactg gcaagcgtcc ggtgaccggt
    17341 aacaaccgtt cccacgcact gaacgcgact aaacgccgtt tcctgccgaa cctgcactct
    17401 caccgtttct gggttgagag cgagaagcgt tttgtcaccc tgcgcgtatc tgctaaaggt
    17461 atgcgtgtaa tcgataaaaa aggcatcgat acagttctgg ctgaactgcg tgcccgtggc
    17521 gaaaagtact aagtacttag aggaaataaa tcatggctaa aggtattcgt gagaaaatca
    17581 agctggtttc ttctgctggt actggtcact tctataccac tacgaagaac aaacgtacta
    17641 agccggaaaa actggaactg aaaaaattcg atccagttgt tcgccagcac gtgatctaca
    17701 aagaagcgaa aatcaaataa ttctcgcttt gatgtaacga aaaacctcgc tccggcgggg
    17761 ttttttgtta tctgcttacc cccatattga ttgcatctgt tcattcctgg agatgctatg
    17821 cctgaattac ccgaagttga aaccagccgc cgcggcatag aaccgcatct cgttggtgca
    17881 accattcttc atgcggtggt gcgcaacgga cgcttgcgct ggccggtttc agaagagatc
    17941 taccgtttaa gcgaccaacc agtgcttagc gtgcagcggc gggctaaata tctgctgctg
    18001 gagctgcctg agggctggat tatcattcat ttggggatgt ctggcagcct gcgcatcctt
    18061 ccagaagaac ttccccctga aaagcatgac catgtggatt tggtgatgag caacggtaaa
    18121 gtgctgcgct acaccgatcc gcgccgcttt ggtgcctggc tgtggaccaa agagctggaa
    18181 gggcacaacg tgctggctca tcttgggccg gagccgctta gcgacgattt caatggtgag
    18241 tatctgcatc agaagtgcgc gaagaaaaaa acggcgatta aaccgtggct gatggataac
    18301 aagctggtgg taggcgtagg gaatatctat gccagcgaat ctctgtttgc ggcggggatc
    18361 catccggatc ggctggcgtc atcactgtcg ctggcggagt gcgaattgct ggctcgggtg
    18421 attaaagcgg tgttgctgcg ttcgattgag cagggcggta caacgctgaa agattttctg
    18481 caaagtgatg gtaaaccggg ctatttcgcg caggaattgc aggtttacgg gcgaaaaggc
    18541 gagccctgtc gggtgtgcgg tacgccgatt gtggcgacta aacatgcgca gcgggcaacg
    18601 ttttattgtc ggcagtgcca gaagtaattc atgcgcgccg gatggcatac catccggcat
    18661 aaacgctacg ctaacttcgc catcagcgcc tgatggacat tctccggcag gaaatgggtg
    18721 acatcgccct gatggcgcgc cacctctttc accaacgatg aagagataaa cgaccactct
    18781 ttcgacggca tcagaaacac actttccagt tccggcatta agtggcgatt catatgcgcc
    18841 agctgcattt catattcaaa atccgccacc gcacgcaggc cacgaatcag caccgtagcg
    18901 tgttgattac gggcgaagtt cgccatcaaa tcactaaacc cgaccacttc cacgttcccc
    18961 agatgcgcgg ttgcctgctg tgccagctca acacgctctt ccagggtaaa catcggtttt
    19021 ttactggggc tggcggcaat cgccagaata acgtgatcga acatctgcgt ggcgcgcgtc
    19081 acgatatcga tatgaccatt ggtaatggga tcgaaagtac ccggataaat cgcccgtttt
    19141 tgcataacaa cctcaatgcg ttttcggtgg caggtaaggt tccagcagtt gaagcagacg
    19201 ctgtagcgcg ccctggtttt gatacagtac ttcaacggca tgacggccat agaaactacg
    19261 gtaatcggcg tcggtgagta aagaggaaac ctcttttgca agcgtagtgg catcggtaac
    19321 ggtaatcagc ccacttgcct gctccagccg cgcgcaaatg tctttaaagt taaaagtatg
    19381 cggccccatc aataccggaa ttgcgtgtgc ggcagcttcc agcggattat gcccaccacg
    19441 ttcaaccagt gaaccgccaa caaaggcgag atcggcaatg ccgtacagta acatcagctc
    19501 gcccatcgta tcgccaacca caacctgcgt gctggaggaa gggacttccc ctgaagagcg
    19561 tgtgatatag cttagtccag cctggcggac aaggttaatc gcatccggga agcgttccgg
    19621 atgacggggt accaggatga gcagtaaatt cgggaattgc tgtaacaatg cctgatgtgc
    19681 ggcgatcacc acactctctt cgccttcgtg agtgctggtg gcaatccata ccgggcggtg
    19741 tggtgcccac tggcggcgca gcgtcacggc tttagcagcc aactgcggcg ttacagaaat
    19801 atcgaatttc aggctaccgg taacggttac ttggttattt tttgcgccca gcgccacaaa
    19861 acgtgcacca tcttcttcat tttgggcggc aatcagcgta atacgacgca gcaagcgacg
    19921 gacgaattta cccagtttgg cataacctgc ggccgagcgg gcagagagtc gcgcgttagc
    19981 gatcaccagc ggaattttac gtttatgtag cgccgcaatc aggttaggcc atagttcggt
    20041 ttccataatc aacaccagtt tagggtcgac tttattcagg aaacggttga gtgcatcggg
    20101 cagatcatac ggcagataaa cgtgctgaac atccttcccg aaagccgatt gtacgcgctc
    20161 cgaaccggtt ggcgtcatgg ttgtaacggt aatcggtaaa tcaggataac gatgacgcag
    20221 cgcgcgcacc aacgggatcg ccgccagagt ttcaccgacg gagacggagt gcagcataat
    20281 gccgcctggt tttagcggat ggcggtaaaa accgtaacgt tcaccccagc gttttcgata
    20341 ggccggagcc ttacgtccgc gcacccagag ccgtatccag atcagcggct gaataaggta
    20401 gagaagggcg gtgtaaagca attcgagcat agtaaatagc tgacttatgg atgtgctggg
    20461 gattctatgt atttagctgt ggctttacca ttacttttcc cgtttttgac ttaaatagca
    20521 atttacccgc caattcttgc tgaacatcta tacttttttt gtatttttat gcgattcatt
    20581 gatttctgct gtacgtgcag gaaggaatcg cagagcaaca gtgaaagaat atggttgcta
    20641 atgattttat ttgattgatt tctttattga aaatcaacaa tgtgcttaaa aatcattcgg
    20701 gtcggattgc cgcgaaagaa gtgatacact agcgcgtcaa tatcagtgcg gtatcagtat
    20761 tcaggtagct gttgagcctg gggcggtagc gtgctttttc tgcttcacct taatcggata
    20821 atctcaacaa aaagagttac ttgtggataa gccatttcga agaattttgc tcattaagat
    20881 gcgttttcat ggggatatgt tattaactac tcccgtcatt agttcgctga aaaaaaatta
    20941 ccctgacgca aaaatcgatg tgctgcttta tcaggacacc atcccgatcc tgtctgaaaa
    21001 tccagagatt aacgcgctct acggcataaa aaataaaaaa gcaaaagcct cagaaaaaat
    21061 tgccaacttt tttcatctca tcaaggtatt acgtgccaat aagtatgacc ttatcgtcaa
    21121 tcttaccgat caatggatgg ttgctatact ggttcgctta ttaaatgccc gtgtgaaaat
    21181 ttcccaggat tatcatcatc ggcagtctgc tttttggcgt aaaagtttca cccatttggt
    21241 gccgttgcag ggtggaaatg tggtggaaag taacttatcc gtgctgacat cattgggact
    21301 tgattcgttg gtgaagcaga caaccatgag ttacccgcct gcaagctgga aacgtatgcg
    21361 tcgcgaactt gatcacgctg gtgttggaca aaattatgtg gttatccaac ctacggcgcg
    21421 gcaaatcttc aaatgctggg acaacgccaa gttttccgct gtgattgatg ccttacatgc
    21481 tcgtggttat gaagtcgttc tgacgtccgg cccagataaa gacgatctgg cctgcgtcaa
    21541 tgaaattgcg cagggatgcc agacgccacc agtaacggcg ctggctggaa aggtgacctt
    21601 cccggaactt ggtgcgttaa tcgatcatgc gcagctgttt attggcgttg attccgcacc
    21661 ggcgcatatt gccgctgcag ttaatacgcc gctgatatcg ctgtttggtg cgacagacca
    21721 tattttctgg cgtccctggt caaataacat gattcaattc tgggcgggag attaccggga
    21781 aatgccaacg cgcgatcagc gtgaccgaaa tgagatgtat ctttcggtta ttccggcggc
    21841 agatgtcatt gctgctgtcg ataaattatt gccctcctcc acgacaggta cgtcgttatg
    21901 atcgttgctt tttgtttata taaatatttt ccctttggcg gtttgcagcg cgattttatg
    21961 cgtattgctc agacagtcgc cgcccgaggt catcatgttc gggtttatac ccagtcgtgg
    22021 gaaggcgaat gccctgatgt atttgaactg atcaaagtgc cggttaaatc gcataccaat
    22081 cacgggcgca atgcggagta ttttgcctgg gtgcaaaaac atttacgcga acatcccgtc
    22141 gataaagtcg ttggattcaa caaaatgccg gggctggacg tttattatgc cgctgatgtt
    22201 tgttatgccg agaaagtagc gcaggaaaaa ggctttttct atcgcctgac gtcacgttat
    22261 cgccattatg ccgcctttga gcgggcaacc ttcgaacagg gcaagccgac acagctgctg
    22321 atgctgacag ataagcaaat cgccgatttc cagaaacatt atcagactga agcggagcgt
    22381 tttcatattc tgccaccggg gatttatcct gatcgtaaat atagccagca gccagccaat
    22441 agccgtgaaa tcttccgtaa gaagaatgga ataaccgaac aacaatattt attgttgcag
    22501 gtcggttcag acttcacgcg taaaggtgtc gatcgttcca ttgaagcact tgcttcgtta
    22561 ccggattcgc tgcgccacaa cacattgcta tatgttgttg ggcaggataa accgcgaaaa
    22621 tttgaggcgc tggcagaaaa acgcggcgtg cgcagtaatg ttcacttctt ctcggggcgc
    22681 aacgatgtct cggaattaat ggcggcggcg gatttattac tgcatcctgc ctaccaggaa
    22741 gcggcgggaa ttgtgctgct ggaagcgatt actgcaggat taccggtact aacaacagcc
    22801 gtttgtggct atgcgcatta tattgtcgag gccaattgcg gcgaggctat tgttgagcca
    22861 ttccgccagg aaacattgaa tgagatttta cgcaaagcgt taacgcaatc ttcattgcgc
    22921 caggcctggg cggaaaatgc gcgacattat gctgatacac aagatttata cagtctgcca
    22981 gagaaagcgg cggatatcat aacgggtggt ctggatggtt gaacttaaag agccgtttgc
    23041 cacgttatgg cgcggtaaag atccttttga ggaagttaaa accttgcagg gtgaggtatt
    23101 tcgtgaactg gaaactcgcc gtactctgcg ctttgaaatg gcgggcaaaa gctattttct
    23161 caaatggcat cgcggcacga ccctgaaaga gataatcaaa aatttactct cattgcggat
    23221 gccagtatta ggcgcagacc gcgaatggaa tgcgattcat cgactgcggg atgtcggcgt
    23281 tgatactatg tatggggtgg catttggcga aaaaggcatg aatccgctga ccagaacttc
    23341 atttattatt accgaagatc tgacaccaac cataagtctg gaagattact gtgctgactg
    23401 ggcgactaac ccttcagatg ttcgcgtaaa gcgtatgctt attaagcgtg tcgcgacgat
    23461 ggtgcgcaat atgcatgctg tgggcattaa ccaccgtgac tgttatatct gtcatttcct
    23521 gctgcacttg cctttttccg gtaaggaaga ggagttaaaa atttcggtaa ttgacctgca
    23581 ccgggcgcag cttcgcacgc gcgttccacg tcgttggcgc gataaagatc ttattgggct
    23641 ttatttttca tcgatgaata tcggcctgac tcagcgggat atctggcggt ttatgaaagt
    23701 gtattttgcc gccccgctta aagacattct caagcaggaa caaggactgc tgtcgcaagc
    23761 agaagcaaaa gccacaaaaa tcagggaaag aacgattcga aaatcgttgt aacccgtaac
    23821 ttatttttgc caaaattttg gatacagaat aaatatgtct caactcaatg atagtgacat
    23881 catccttttt gagtataatt ttcattatca aaatataaga tctaaaaata ctcttgatat
    23941 agcatttggt attgacagaa attttctttt t
//
LOCUS       DAZGCQ010000076        23758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000076 DAZGCQ010000000
VERSION     DAZGCQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.076"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..462)
                     /locus_tag="ACHK07_003353"
     CDS             complement(<1..462)
                     /locus_tag="ACHK07_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO8103642.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAIATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDD"
     gene            complement(871..1194)
                     /locus_tag="ACHK07_003354"
     CDS             complement(871..1194)
                     /locus_tag="ACHK07_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO8103643.1"
                     /translation="MKRTLLIAVWAIGLMSDGAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(1202..1387)
                     /gene="ynbE"
                     /locus_tag="ACHK07_003355"
     CDS             complement(1202..1387)
                     /gene="ynbE"
                     /locus_tag="ACHK07_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO8103644.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(1384..4023)
                     /gene="ydbH"
                     /locus_tag="ACHK07_003356"
     CDS             complement(1384..4023)
                     /gene="ydbH"
                     /locus_tag="ACHK07_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO8103645.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVGAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            4231..5220
                     /gene="ldhA"
                     /locus_tag="ACHK07_003357"
     CDS             4231..5220
                     /gene="ldhA"
                     /locus_tag="ACHK07_003357"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8103646.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5331..5753
                     /gene="hslJ"
                     /locus_tag="ACHK07_003358"
     CDS             5331..5753
                     /gene="hslJ"
                     /locus_tag="ACHK07_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO8103647.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5750..6016)
                     /gene="ydbJ"
                     /locus_tag="ACHK07_003359"
     CDS             complement(5750..6016)
                     /gene="ydbJ"
                     /locus_tag="ACHK07_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO8103648.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            6290..9814
                     /gene="nifJ"
                     /locus_tag="ACHK07_003360"
     CDS             6290..9814
                     /gene="nifJ"
                     /locus_tag="ACHK07_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO8103649.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKVRF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            10179..11312
                     /gene="ompN"
                     /locus_tag="ACHK07_003361"
     CDS             10179..11312
                     /gene="ompN"
                     /locus_tag="ACHK07_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO8103650.1"
                     /translation="MKSKVLALLIPALIAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            11453..11887
                     /gene="uspF"
                     /locus_tag="ACHK07_003362"
     CDS             11453..11887
                     /gene="uspF"
                     /locus_tag="ACHK07_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO8103651.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            12063..12998
                     /gene="ttcA"
                     /locus_tag="ACHK07_003363"
     CDS             12063..12998
                     /gene="ttcA"
                     /locus_tag="ACHK07_003363"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO8103652.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(13127..14500)
                     /gene="dbpA"
                     /locus_tag="ACHK07_003364"
     CDS             complement(13127..14500)
                     /gene="dbpA"
                     /locus_tag="ACHK07_003364"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO8103653.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQQLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGYIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(14530..14703)
                     /gene="ynaL"
                     /locus_tag="ACHK07_003365"
     CDS             complement(14530..14703)
                     /gene="ynaL"
                     /locus_tag="ACHK07_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO8103654.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(14978..15961)
                     /gene="zntB"
                     /locus_tag="ACHK07_003366"
     CDS             complement(14978..15961)
                     /gene="zntB"
                     /locus_tag="ACHK07_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO8103655.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(15975..16022)
                     /gene="ydaN"
                     /locus_tag="ACHK07_003367"
     CDS             complement(15975..16022)
                     /gene="ydaN"
                     /locus_tag="ACHK07_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO8103656.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            16215..17447
                     /gene="dgcM"
                     /locus_tag="ACHK07_003368"
     CDS             16215..17447
                     /gene="dgcM"
                     /locus_tag="ACHK07_003368"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO8103657.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLVA"
     gene            complement(17468..18031)
                     /gene="smrA"
                     /locus_tag="ACHK07_003369"
     CDS             complement(17468..18031)
                     /gene="smrA"
                     /locus_tag="ACHK07_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO8103658.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(18361..19269)
                     /gene="abgR"
                     /locus_tag="ACHK07_003370"
     CDS             complement(18361..19269)
                     /gene="abgR"
                     /locus_tag="ACHK07_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8103659.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            19445..20755
                     /gene="abgA"
                     /locus_tag="ACHK07_003371"
     CDS             19445..20755
                     /gene="abgA"
                     /locus_tag="ACHK07_003371"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO8103660.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            20755..22200
                     /gene="abgB"
                     /locus_tag="ACHK07_003372"
     CDS             20755..22200
                     /gene="abgB"
                     /locus_tag="ACHK07_003372"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO8103661.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPISKNVTPSPLK"
     gene            22237..>23758
                     /gene="abgT"
                     /locus_tag="ACHK07_003373"
     CDS             22237..>23758
                     /gene="abgT"
                     /locus_tag="ACHK07_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO8103662.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRL"
BASE COUNT         6013 a         6024 c         5773 g         5948 t
ORIGIN      
        1 gtcatctggc ggaactggcg tatcaccgcc gtcatctggc ggaactggcg tatcaccgcc
       61 gtcatctggc ggaaccggcg tatcaccgcc gtcatctggc ggaaccggcg tatcaccgcc
      121 gtcgtctggc ggaacaggcg tatcaccgcc atcatccgga actggggagt tattatggtg
      181 gtgattgtca tcgtcactta aggtatatat agcaattccc gtagctattg cagcaagacc
      241 tgcagctgcc catggtaaaa ctgcactatc tttattttct ctgcactccg cggataaatt
      301 ttctagctgc tctttgctca gggaatgaat atttgccgga catgttatct tactggactt
      361 acgtgttttt tcggttgagt cgggtattga agtatccgca gcccatgtct ggctacttaa
      421 cgccatggca acacaaacag acaataattt tttcctttgc atataaaatt ccttagatct
      481 agaccgtgcg taacaagcca tgcagaattt gaagataaat cgtagtaaat attcatagtg
      541 aagtaagcaa taaagaaatg cttatataat tcacaaaatc aatattaaaa tcaggcaagt
      601 aaaagcatac aaaaaaaaca gttgatggaa ttatattcca caaaaaactc acttcaatct
      661 aaaaattaac gacaaaaaac aaaaacacac taataattca ttgacgctgc atatatattt
      721 tgtgattaca tatatattag taatgatttg ttatttaagg gaaggatata taaggttttt
      781 gtagaaatac ccacggtaat taagcatgct aacgtcagtt taaatactgt aaaagatcat
      841 taatagatac attttgtagc caactataat ttatttgcgc aaccatttac cattaactcc
      901 ctgaacatat tgccccgact tcgcgcgagc aacaagcttt tgtccagcca gtttggctat
      961 ctctttagtg gatacattat tttgctttgc cagttgttgg taactatggt tacgagcttc
     1021 attaatatct tttactaatg cctgtgtttc ggcatccgtt tgtagcgcca ccaggtaacc
     1081 gttaagcgtt tcacctaccc gcccctgact tctggcttcg ttcagggtta aagccattgc
     1141 accgtcgctc atcaggccaa tggcccatac agctatgagt aatgttcttt tcatcatcac
     1201 ctcaaaatag atcgctacgg gttttgagca ggtcttcaac gtccttatcc gctttaatga
     1261 tgatttcatg ctcaatttta acgttcatat taatggtgat tggctctttc ggcgcagcca
     1321 cttcaatgcg aggcgtgcaa ccaaccaaca taaaagagga cgcaaacgcg gctatcacta
     1381 ttttcattgt ttttcctcac actcttttcc tgatgggcag ccagtttccg gtagctgggc
     1441 attctgctcc agccatacct ggagattatc gccaaagcgt aaactgcgcc ataacgtaaa
     1501 aatattctct tcatggtgat aattaaggtt taccgtaccg cttttaccat cgacgcgact
     1561 ggtgcccgaa atgttggcct gcatggttaa cacaccgaga ttatctaaat taatccttgt
     1621 tgatgagcga ctgatttcca tataacgcaa ccagttgatg gcagttccag cggtcatatt
     1681 gtctttggcc accgcatctg cagtgtcttt atccagacgc agcgtcatcg gccccgcgtt
     1741 tgccaaccag ccatctttga caatccattt ttcattgttc agccatagtg gcaacgcccc
     1801 actaaatgcc ccagacatag caaactgttt cggattaacc gcgctaacca gttcgctgga
     1861 tgagagatta ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat
     1921 cagcacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaatg gttcttcttc
     1981 agtccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat
     2041 cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc
     2101 aaaacggaaa ggcaggacaa aatcgacacc gttcacctga ttatctggca tccaggcact
     2161 accgcctttc aacacgccgt gccctcccgc gcggaatcct tgttcaggcg cagcagaaaa
     2221 tgcaacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccag
     2281 cggctggaat acagtcagtg attgtttagg ccaccaggca ttaccgcgca gacgaatacc
     2341 gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa
     2401 gaggaaatac gtcggatcgc gcccatcgac gctaaatttt aaggttgatg gcggtaacac
     2461 actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg
     2521 atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac
     2581 cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca gcgtaatcgt
     2641 gctgtcatgc cactcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact
     2701 cccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat cattcgccag
     2761 cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg
     2821 caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc
     2881 atcgatgtcc agcgaatcga tgactcttcc ctttgaacga agtaacgcgc ctggctcaaa
     2941 ggtcagcgtt gggtcagaca gacttccact tacctgcgca ggtaaacggg catataaaat
     3001 gaggtccgcc tgtttcgctt caccggtcag ctgcagaggc agctgactgt tatccatgct
     3061 taattttcct gggccaaaat ttagcaccgc gttgccttta cccgcttgcc cctgggtcag
     3121 cacactcagt cgtccgctga ccagagcgtt ctcaagccct gcctgccagt tgtcgacttt
     3181 gacacccagt cggccactca aaggaaaacc tgcatacggc cagctccagc gaccatcgct
     3241 tacggtcaat tgttgacgcg taatttgcca cggcaaatcg agcaacggat cgccgttatc
     3301 ccgtgccagc acgatcaatt gcccgctatt ttcctgccag tccagctcgg cacccaccag
     3361 tgacggttcc tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag gaagtccatc
     3421 cggcacgagc ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc
     3481 gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac
     3541 tttttcgccc tgataacgca gttgctggat atcagaggtt aatgcgagag agagttttcc
     3601 ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag
     3661 catggactgc cactgcgcga gggtttttgg agcagctggc gattgctccg tctgcggcaa
     3721 tttcgccaga caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg
     3781 atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag
     3841 atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt
     3901 cccgagcggt agccagatgc ccgccagcgt gggaacccac agcccgagcg tcatcagcag
     3961 cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag
     4021 catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc
     4081 atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt
     4141 caatatcgcc atagctttca attaaatttg aaattttgta aaatattttt agtagcttaa
     4201 atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag
     4261 tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt
     4321 gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt
     4381 ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa
     4441 tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg
     4501 gggctgaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc
     4561 ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct
     4621 aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc
     4681 ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg
     4741 ctggcgttcg atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat
     4801 ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa
     4861 aactaccatc tgttgaacga agccgccttc gatcagatga aaaatggcgt gatgatcgtc
     4921 aataccagtc gcggtgcatt gattgattca caggcagcaa ttgaagcgct gaaaaatcag
     4981 aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagat
     5041 aaatccaacg acgtgatcca ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg
     5101 ctgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact
     5161 acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa
     5221 tcttgccgct cccctgcatt ccaggggagc ttattcagat aatccccaaa gacctttcat
     5281 cctctattct taaaatagcc ctgagtcaga aactgtaatt gagaaccaca atgaagaaag
     5341 tagccgcgct tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg
     5401 ctgttacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc
     5461 ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg
     5521 gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca
     5581 aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca
     5641 ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc
     5701 tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc
     5761 cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca
     5821 tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac
     5881 aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag
     5941 tcgcctgctg aacaggctca ctactacacg ccgacaacaa taaagcggca caccctaccc
     6001 aaaacgctgc tcgcatctct tcttcctctg atcttcaagc caaacgacac cgccataaat
     6061 aataggcagc acagagggcg acgtcgagag ctgtcctgcg cgttgccccg catttttact
     6121 tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg
     6181 gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaagat tactactggg
     6241 cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat
     6301 tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta
     6361 ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt
     6421 gaagaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag cgggtgctat
     6481 cgctaccgtg catggcgctt tgcagacggg tgccctttca acatcgttta cgtcatcgca
     6541 gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactca caccgtttgt
     6601 cctgcatgta gcggcacgta ccgttgccac acatgcactc tctatttttg gcgatcattc
     6661 cgacgtcatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcaa acgtccagga
     6721 agcgcaagac tttgctctca tttcgcacat cgcgacgctg aaaagccgcg tgccatttat
     6781 tcatttcttt gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc cgctggccga
     6841 tgacacgatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcact
     6901 caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc
     6961 tcgcgaagcc accaacccat ggtacaacgc ggtctatgac catgttgaac aggcgatgaa
     7021 tgatttcgct gccgcgacag gtcgtcagta tcagccgttt gaatattacg ggcatccgca
     7081 agcggaacgg gtgattatcc tgatgggctc tgccattggt acctgtgaag aagtggttga
     7141 tgaattgcta acccgtggcg aaaaagttgg cgtgctgaaa gttcgtctgt accgtccctt
     7201 ctccgccaaa cacttgctgc aagctctgcc gggatccgta cgcagcgtgg cggtactgga
     7261 cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtaa tgaccgcact
     7321 ggcagaagcc tttaataatg gcgagcgcga aactctgccc cgtgtcattg gtgggcgcta
     7381 tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa
     7441 cgcggctaaa ccgaaagtgc gctttacggt tggtatttac gatgatgtga ccaatctgtc
     7501 actgccgttg ccggaaaaca ccctgccaaa ctcggcgaaa ctggaagcct tgttttatgg
     7561 gcttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc
     7621 cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac
     7681 ggtttctcac cttcgagtga gcgaacagcc gattcgttcc gcttatctca tttcccaggc
     7741 tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt
     7801 aaaacctggc ggcattttcc tgctcaacac gccgtacagc gcagatgaag tgtggtcgcg
     7861 cttgccgcag gaagttcagg ccgtgctaaa tcagaaaaaa gcgcgcttct atgtgattaa
     7921 cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat
     7981 ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg
     8041 tgcgattgcc aaaagttaca gtagcaaagg tcaggatctg gtggaacgca actggcaggc
     8101 tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg cagccggtta atccgcacag
     8161 cgccaatcga ccgccagtgg tttccgatgc cgcccctgat ttcgtgaaaa ccgtaaccgc
     8221 ggcgatgctc gccgggcttg gtgatgccct ccccgtttcg gcgctgccgc cagacggcac
     8281 ctggccgatg ggcactacac gctgggaaaa acgcaatatc gccgaagaga tccccatctg
     8341 gaaagaggaa ctctgtaccc aatgtaacca ctgtgttgcc gcttgcccac actcagctat
     8401 tcgcgcaaaa gtggtaccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct
     8461 ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg ctccggaaga
     8521 ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga
     8581 gattaaagcc atcaatatga tgtcgcgcct tgaacatgtc gaagaagaga aaatcaatta
     8641 cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg
     8701 tacatcgcag ctgattacac cgctgtttga atattccggt gcttgctccg gttgtggcga
     8761 gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaacgc
     8821 caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata ccaccgatgc
     8881 caacggtcgt gggccggcat gggcgaactc tctatttgaa gataatgccg aatttggcct
     8941 tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt
     9001 tgccgataaa atcccggcgg aattactgac ggcgttgaaa tcagacgcta cgccagaggt
     9061 tcgtcgagaa caggtcgcag ctttacgcca gcaactcaac gatgttgccg aagcacatga
     9121 actgctgcgt gacgcagatg cactggtaga aaaatcaatc tggctgattg gtggcgacgg
     9181 ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt
     9241 caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg cgtcgaaagc
     9301 gacaccgctg ggtgcagtaa ctaagttcgg cgagcacggc aaacgtaaag cgcgtaaaga
     9361 tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc
     9421 gcaactgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg gaccatcgct
     9481 gatcatcgct tatagcccat gtgaagagca tggttacgat ctggcactca gccatgacca
     9541 gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgcttcgatc cgcgtcgtgc
     9601 cgatgaaggc aaactgccgc tggcgctgga ttcacgcccg ccgtcagaag cactggaaga
     9661 aacgttactt cacgagcaac gtttccgtcg tctgaattcg cagcagccag aagtggcaga
     9721 acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat
     9781 ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga ttatccaatt
     9841 ctaaaaaaag cccggacgac tgttcgggct tgtcttttta tatgttggaa aatcagtggc
     9901 aatgcaatgg cccaacagaa aataaaatct gtgacaataa aggcatataa cccgcgcaga
     9961 ataacgtata gaaaataaaa agtataattt ttattttgta tgtattatct tcgatacatt
    10021 caccagactt attatcattt cagaggaatt atttgattaa ggttttactt aaggcgtaac
    10081 aaatgatatt tatcggctaa ctgaacttct cctttaggat gttttcactc cccgaaatgg
    10141 ggataaaagg caaataaaat aactaaagga tttattcaat gaaaagcaaa gtactggcac
    10201 ttttaattcc tgccctgatc gccgcaggtg ctgcacatgc agccgaagtt tataataaag
    10261 acggcaacaa attagatctg tatggcaaag ttgatggcct gcattatttt tctgataatt
    10321 cagcgaaaga tggcgaccag agctatgcgc gtctgggttt taaaggcgaa acccaaatta
    10381 acgatcaact tactggctac ggtcaatggg aatacaatat tcaggcaaac aacactgaat
    10441 cttcaaaaaa ccagtcatgg acccgtctgg catttgccgg actgaaattt gcagattacg
    10501 gttctttcga ttacggacgt aattatggcg taatgtacga catcgaaggc tggaccgata
    10561 tgctgcctga atttggcggt gactcttata ccaatgcaga caactttatg actggtcgtg
    10621 ccaatggcgt cgcgacttat cgtaatactg atttcttcgg tctggtaaat ggtctgaact
    10681 tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa tggtcaggaa ggcaccaaca
    10741 acggacgtga tgttcgccat gaaaacggtg acggctgggg tctttccaca acatatgatt
    10801 taggcatggg ctttagcgct ggtgcggcat acacctcttc tgaccgcacc aatgaccagg
    10861 ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg gactgctggg ctaaaatacg
    10921 atgctaacaa tatttacctg gcaaccatgt attcagaaac gcgtaatatg accccgtttg
    10981 gcgacagcga ttatgcagtt gcgaacaaaa cccagaactt tgaagtcact gcacagtacc
    11041 agtttgattt tggtctgcgt ccggcagtct ctttcctgat gtctaaaggc cgtgacctgc
    11101 acgctgccgg tggtgcagac aacccggcag gtgttgatga taaagatctg gttaaatacg
    11161 ccgatgttgg cgcgacttac tatttcaata aaaacatgtc cacttatgtt gactataaaa
    11221 tcaacctgtt ggatgaagat gacagtttct acgctgccaa cggcatctct accgatgata
    11281 ttgtcgcttt aggtctggtt tatcagttct aaatcctcct gcccgctgtt atggcgggct
    11341 ttttctgctt attcttcctc ctttgatcta aattaaaaat gtgaactccg tcattacaca
    11401 aaaagtgtca tctggcgtta cactttatgc ggatactaaa acaggaggtt ttatgaacag
    11461 aacgattctt gtccctatcg atatttccga ttcagaatta actcaacgcg tgattagcca
    11521 cgttgaggca gaggcaaaga ttgatgatgc agaggttcat ttcctgacgg taataccttc
    11581 actgccctac tatgcctctc tgggtttagc gtattccgca gaattaccgg caatggatga
    11641 cctgaaagcg gaagccaaat cgcaactgga agagatcatt aaaaaattta aactgccaac
    11701 cgacagagtg catgtccatg ttgaggaagg ttcgcccaaa gaccgtattc tggaattggc
    11761 gaagaagatc cccgctcata tgatcatcat tgcttcccat cgaccggata tcaccactta
    11821 tctgctcggt tccaacgccg cagctgtagt gcgtcacgca gagtgctccg tgctggttgt
    11881 gcgctgacac taacgcccgc acattgctgc gggctttttg attcatttcg caaatgtgct
    11941 gacattttcc cctctaatcc gtaccataca cgccacagtt tttatatcag atttcttatg
    12001 ctgggcgttc cggcatgacg catactcttc tgatgccata taacgaattg agtcgctttt
    12061 aaatgtcgca aaatcaagaa attagcaaga aagaacaata caatctgaac aaattacaaa
    12121 aacgcctgcg tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt gaagaaggcg
    12181 atcgcatcat ggtttgcctc tccgggggta aagacagcta taccatgctg gagattctgc
    12241 gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa
    12301 agcaaccggg cttcccggaa cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt
    12361 acaagattgt tgaagagaac acttacggta tcgtgaaaga gaagattcca gagggcaaaa
    12421 ccacttgctc actgtgttct cgacttcgtc gcggtatcct ttatcgcacc gcaacggaac
    12481 tgggggcgac gaagatcgcg ttgggccacc atcgtgacga tatcctgcaa acgttgttct
    12541 taaatatgtt ctacggcggc aagatgaaag gtatgcctcc gaaactgatg agcgatgatg
    12601 gcaaacatat cgttattcgt ccgctggcct actgccgcga gaaagatatt cagcgatttg
    12661 ccgatgcaaa agcgttcccg attattccgt gcaacctgtg cggttcacag cctaacctgc
    12721 aacgtcaggt gattgctgac atgttgcgtg actgggataa acgttatcca gggcgtatcg
    12781 agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt
    12841 tcgatttcaa aggcatcacc cacggttctg aagtggttaa cgggggtgat ctggcgtttg
    12901 atcgcgaaga gatcccacta caaccggcgg gctggcagcc agaagaagat gaaaatcagt
    12961 tggatgagtt acggctgaat gtggttgaag tgaaataacc aggatagcgc ccgatgcgca
    13021 agcgtatcgg gctactctta tggaggccgg ataagacgca gccagcgtcg catccggcaa
    13081 tcccgaataa gatgtttact cttgcacccg gcaattcaac atttcattat tttaataacc
    13141 gcacccggca cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat gctttatgag
    13201 caacagcctg acggaccgcg acatagacat gcgccggatg cacggcgatt ttgccaatat
    13261 ctgcgccatc aagcccgata tatcctgtca gtgcacctaa tacatcaccc gggcgcattt
    13321 tggctttttt cccgccatcg atacacaacg ttgccatttc tgcttccagc gtcacaatgg
    13381 aactattagc tggcggcgtt tgccagttaa gttttatctg caacatgtca gaaatgatat
    13441 tggcccgctg tgcttcttcc ggagcacaga aactgatcgc cagaccgcta tttcctgcac
    13501 gagctgtacg accgatgcga tgtacatgaa cttcagggtc ccacgccagc tcaaagttca
    13561 ccaccagctc aagcgattta atatccagac cacgcgcagc aacatcagtc gcgaccagta
    13621 cacgggcgct accgttagca aaacgtacca gggtctgatc gcgatcgcgt tgctccaaat
    13681 cgccgtgtaa tgacaatgca ctttgcccta cttcattcag cgcgtcgcag acagcctggc
    13741 aatctttttt ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag cttaataact
    13801 gttgcaacag aggaattttg cctttactgg atgtctcata aaattgttgt tcaatgggtg
    13861 gcaaagcatc tgttgagtca atttcaatcg ccaaaggatc gcgttgcact cgtccgctga
    13921 ttgcggcgat ggcttccggc caggttgccg aaaacagaag cgtctgtcga gatgccggcg
    13981 caaaacggat gacatcatca atggcatcgc taaatcccat atccagcatg cggtcggcct
    14041 catccatcac caaagtattc agcgcatcca gtgataccgt gcctttttgc aggtgatcca
    14101 gcaaacggcc cggcgttgcc acgataatat gcggcgcatg ttgcaacgaa tcacgctgca
    14161 taccgaacgg ttgaccaccg cacaacgtca aaattttggt atttggcaga aaacgcgcca
    14221 gccgacgcaa ttcacctgcc acctgatccg ccagttcacg cgtaggacac agcactaaag
    14281 cctgggtttg aaatagcgac gcatcaattt gctgtaacaa gccgaggcca aaagccgccg
    14341 ttttgccgct gccagttttc gcctgcacgc gaacatcttt tccggcaagg atcgccggaa
    14401 gcgcggcggc ctgcaccggc gtcatggtta aataacccaa ctcattaagg ttcgtgagtt
    14461 gggcgggagg caaaacattc agggtagaaa aagcggtcac aatctattct cgtggtcatc
    14521 gacgcaaagt tagcaggcgc gtatcctcgc agatctacgc tcacgatgcg acaatttaat
    14581 cggttcttca tcgggtggtg ggtcaggcat gggttgcggg cgagggatcg gatcgggcac
    14641 tggaacagga tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa taagtgtcgt
    14701 catatttccc tccggtcatt gggtggactc ttaaagggta gacgctgata aataacaggc
    14761 aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc aattgtgcta tgcagtaatt
    14821 caaaaaagga agtaagacaa tatggagcgc aacgcccatc gcttgacgtt gcattcacct
    14881 gcaagagaga tattgccctg aatgggtaga gagtttattg acttcgctca aactttgcgg
    14941 cgtttttgta tacagatagc cggaaaaatt gcttttgtta caaccattta ctacgatgca
    15001 accataaagc aacaccacca ataagaacaa ctaacagaat acaaaaaatt gaaaatccga
    15061 attgccaccc gccgccaggg atcccaccaa ggttgacgcc aaataaccct gtcagaaagg
    15121 tactgggtaa aaagaccatt gccatcaatg acattgtata ggtacgacga gctaaatttt
    15181 cctgcatcac ctgagcgatt tcatccgcca tcacaccagt ccgtgctata caggcgtcga
    15241 tttcgtcaag gccgcgccca aggcgatcgg caatatcctg catccgacgg cgttggtcat
    15301 cgctcatcca cggcaaacgt tcactggcaa gacgagcata gacatcacgt tgcggtgcca
    15361 tatagcgacg catcacaatt aattgtttgc gcagcagagc caggaatcca cgcggcggaa
    15421 tttgctgatc aaggagatta tcttcaaggt cgataatttt atcgtgcagc tgctcgataa
    15481 attcgctgga atgatcggtc aacgcatcgc acacatccac cagccaccct ccgcaatcgg
    15541 tcggacccgt gccctcttcc agatcgctca ccacatcgtc cagcgccaac actttgcgtt
    15601 gtcgggtcga aacaattaac cgcccgtcca tatatacacg catggcgacc agttgatcgg
    15661 ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct tcaccgagac
    15721 ggctgactcg gggccgtgtg ctctcacctg ccagcgcatc acgtacgtta ttgggaagca
    15781 gcggtgttgt cgccagccat tgggcgctat catggtgtac ataattaagg tggagccagc
    15841 agggatgcgc ttcatcaatc acatctgtat tttccagcgg tttaacgccg cctctaccat
    15901 ccagcatcca ggcaaatact gcatccggga cattaacgtc cgatccctta atcgcttcca
    15961 cagtgcctcc atcatcaacg cattattttg tagtctagcc ttctggccct gttacgcaac
    16021 atctcatcac cccattaccc tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa
    16081 agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct atcattatca
    16141 gaattatctg atcatatgac gtgctttttt gcgatcggat agcaacaaaa attgataaaa
    16201 ataacgggat ctcaatgatt acgcacaact tcaataccct ggacttactc accagtcctg
    16261 tctggatcgt ttcgcccttt gaggaacagt taatttatgc caatagcgcg gcgcgactgt
    16321 tgatgcaaga cctcacgttt agtcagctac gaaccggtcc ctattccgtc tcctcacaaa
    16381 aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca
    16441 ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg cgagagctga
    16501 cagaaacaga accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa
    16561 aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc
    16621 tgactaactc tgcaccaatg ttgttgattg acccgtcacg agatggccaa atcgtcgatg
    16681 ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc cagaaacata
    16741 cctgggaaat aaatatgctc gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt
    16801 tacccggcgg tcataaacct ttgaattttg ttcataaact ggcggatgga tctactcgtc
    16861 atgtgcagac ctatgccgga ccgattgaaa tttatggcga caagctcatg ttatgtattg
    16921 tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct gctcaccatg
    16981 acgcgatgac cggattactg aatcggcgac agttttatca cattacggaa ccaggccaaa
    17041 tgcagcatct cgccatcgct caggattaca gcttgttgct catcgacacc gatcgtttta
    17101 aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc
    17161 gcaccctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt
    17221 ttgtcttatt gctaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc
    17281 gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc agcattggtg
    17341 tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt
    17401 tgtatcgggc gaaaaatgat ggtcgcaacc gcgtgctggt ggcataagcc gcggatgcgt
    17461 ctcgagatca acgactgcgc ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg
    17521 ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtgcaat
    17581 atgcctgaac atcatcaaat tcggtcagcc agcgcgccac atagctgcgg acaatattgg
    17641 catgcgattt atcatcccgc cctttaccat gaataatcag cacgttacgc aaaccatccg
    17701 ccagggcttg ttgaataaaa ctgaacacca ttttgcggca ctcttccacc ggctggcgca
    17761 aaagattcag gctcgcctgt tgcggatatt taccactacg cagcttatcc agcaccccat
    17821 gttgcaaccc ttcccgccga aactccagcg gctgacttag cgggataatg tcgagaaatc
    17881 cggtagtgag gaaattatca agctgcagcg tgtcgatacg ctgcggcgca cgttggttac
    17941 gcgttggatg ccagtggaca tcggtagcac gtttcagcgg ctggacatct tccatggcgt
    18001 caagaaacag cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt aagagcgcga
    18061 tatgataacc agaccgggtc ggtccaacaa cgtattaccc aaaattccag taataagttc
    18121 caaatattgc cgatatttta agcaaaatgc ttatgcatga ttattcattc acgatattaa
    18181 taatgtaact tatattttcg tgaaatctgt cactgaagaa aattggcaac taaaggttaa
    18241 aaccgttata acacagtcac cggcgcagag gagacaatgc cggatttaag acgcggatgc
    18301 actgctgtgt gtactgtaga gtctggcgga tgtcgacaga ctctattttt ttatgcagtt
    18361 ttaactttgc agatagccgc attctcgccg gaattgcgtg attaatgacg cggtcagtgg
    18421 tgtctggcga ctatcacgcc gctgaatcaa ataataggcc gctttcggta aaatttctct
    18481 aaccggcaac atcaccagcc cctgtccgtg caagggatcg cagcccattt cttcaggcag
    18541 tatgctgaga aaatcgcttt ttgccaccag actgatacag gctgagaacg tctcgcagac
    18601 tacaccgacc tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact gtttgtagta
    18661 gctgccgtgt ggcgtcggca ttgtccagct gtaatccagt aactgtttga tcgaacgggc
    18721 accaatggcg gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca gtaatttctc
    18781 aaaagtaaat tcgtggtcgt acggtccctg ataataggta ttgatggtga aatccaattc
    18841 tccctgacgc aattcattaa tcatcgacac cagttgccct tccataatgc gtacttttac
    18901 ctgcggatgc tgctgatgaa aacgagatat gacagctggc atcagactgc gggaaatact
    18961 ggcccccatg ccgatattaa tctgccctgc cagttgccct tgtcgttggc gaatatcctc
    19021 ttgggctgcg cgcagctctt caagaattag actggcgtgc tgataaaaac tttcaccggc
    19081 atcagttaac gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta acccttcttc
    19141 tagctcctga atagatttac tcagtgccgg ttgcgacata ttcaacattc ggctcgctcc
    19201 gcgaatgctg ccctgacgag ccacttcaac aaaagcccga atttgatgaa tttttacctg
    19261 aaaagccatg acgccaccga taaccgttat ttatcagacc aaagaaactg gcatctactt
    19321 taatgcagat gattgtgtca gggtaattta tgaacggtta aaactgtgaa aaatcagtta
    19381 gtgataagta aaaactatcg ctacgtgaac cgggtcacac tttttactga tgacgggaaa
    19441 ggttatggag tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat cgcactggcg
    19501 acgtgatttt catcactatg cagagtctgg ctgggtggaa ttccgcactg ccacccttgt
    19561 tgcggaagaa ttgcaccagc tcggctattc actggcgctg ggtcgcgaag tagttaatga
    19621 aagtagccgg atgggattac ctgatgaatt cactctacaa cgcgaattcg agcgcgctcg
    19681 tcaacagggt gcgctagcac aatggattgc ggcttttgaa ggtggtttca ctggtatcgt
    19741 cgccaccctg gataccggtc gccccggtcc ggtgatggct ttccgtgtcg atatggacgc
    19801 gctggatctc agtgaagagc aggatgtcag ccatcgcccc taccgcgacg gttttgcgtc
    19861 atgtaacgcc ggaatgatgc atgcctgtgg tcatgatggg cataccgcca ttgggcttgg
    19921 gctggcgcat acccttaaac agttcgagtc cggactacat ggcgtcatca aactgatttt
    19981 tcagcctgca gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag gtgtagtgga
    20041 tgatgttgat tattttactg ccgtgcacat tggcactggc gtacctgcgg gcaccgtggt
    20101 gtgcggcagt gataatttta tggcaaccac caaatttgac gcgcacttta ccgggaccgc
    20161 cgctcacgca ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg cagcacaagc
    20221 aactcttgca ctacatgcaa tcgccccgca cagcgaaggc gcttccagag taaacgtggg
    20281 cgttatgcag gcaggaagcg gtcgtaatgt tgttcctgcc tcggcgttgc tgaaagtgga
    20341 aacacgcggg gccagcgacg tcattaatca atatgttttt gaccgtgctc agcaagcgat
    20401 tcagggcgca gcaaccatgt atggtgtcgg cgttgaaact cgtctgatgg gtgcagctac
    20461 cgccagttct ccttcgccgc aatgggtcgc atggttgcaa agtcaggcgg ctcaggtcgc
    20521 gggggtcaat caggccattg aacgtgttga agcgcctgcg ggttccgaag atgccacatt
    20581 aatgatggcc cgcgtgcagc aacatcaagg gcaagcctcc tacatggtgt ttggcacaca
    20641 gctggcggca ggtcatcaca acgaaaaatt cgattttgac gagcaggttc tcgctattgc
    20701 cgtcgaaacg ctggcgcgca ccgcgctcaa ttttccctgg acgcgaggta tctgatgcag
    20761 gaaatctatc gttttatcga cgatgcgatt gaagccgatc gccaacgtta taccgatatt
    20821 gccgatcaaa tctgggatca tccagaaaca cgttttgaag agttctggtc agcggagcat
    20881 ctggcttcgg cgctggaatc tgcaggcttc accgttaccc gcaacgtagg caatatccca
    20941 aatgccttta ttgcttcgtt tggtcaaggc aaaccggtta tcgccctgct gggagaatat
    21001 gacgccctgg caggtttaag tcagcaagca ggttgcgcgc aacctacatc cgtgacgccc
    21061 ggtgaaaatg gtcacggttg cggacacaat ttgctgggaa ccgccgcctt tgccgctgca
    21121 atagccgtca agaaatggct ggaacaatat gggcaaggcg gcacggtgcg cttttatggt
    21181 tgtcctggcg aagaaggcgg ctcgggtaaa acgttcatgg ttcgcgaggg ggtatttgat
    21241 gatgtggatg cggcactcac ctggcacccg gaagcctttg ccggtatgtt caatacccgt
    21301 acgctggcaa acattcaggc atcatggcgc tttaaaggga tcgcagcaca tgccgcgaat
    21361 tcccctcatt tgggacgcag cgcccttgat gccgtaacgt tgatgaccac tggcaccaac
    21421 ttcctcaacg aacatattat tgaaaaagcg cgcgtacact atgccatcac aaatagtggc
    21481 gggatctcgc ccaacgtggt ccaggcgcag gcagaagtgc tttatcttat ccgcgccccc
    21541 gaaatgaccg acgtgcagca tatttatgat cgagtcgcca aaatcgccga aggtgcggca
    21601 ttgatgaccg aaaccacggt tgaatgccgc ttcgacaaag cctgttccag ttatctcccg
    21661 aatcgcacct tagaaaatgc catgtaccag gccctatccc attttggtac cccggaatgg
    21721 aactccgaag aactggcttt tgcgaaacaa attcaggcta cgctcacccc caacgatcgg
    21781 caaaacagtc tgaataatat cgccgcaacc ggtggcgaaa acggcaaggc ttttgcacta
    21841 cgtcatcgtg aaacggtact ggcgaatgaa gtcgctccat atgccgccac cgataacgtg
    21901 cttgcggcat cgactgatgt cggcgacgtc agttggaaac tgcctgttgc ccagtgtttc
    21961 agcccctgtt ttgccgtcgg tacaccgcta catacgtggc aactggttag ccaggggcga
    22021 acatctattg ctcataaagg aatgctgctg gcggcgaaaa ctatggcagc aaccacactc
    22081 aatctcttca ttgattcagg gctattgcaa gaatgccaac aagagcatca gcaagttacg
    22141 gacacgcaac cgtatcactg ccctatctcg aaaaacgtga caccgtcacc tttaaaataa
    22201 caacaacaac gcaaacacaa caaccgagga atgcccatga gtatgtcatc cataccgtcg
    22261 tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa ttggtaacaa ggttccccat
    22321 ccttttttgc tctttatcta tttgattatc gtactcatgg tgacgacggc aattttgtcg
    22381 gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt gaaaaacctg
    22441 ctcagtgtgg aaggattaca ctggttttta cccaatgtta ttaaaaactt tagcggtttt
    22501 gctccacttg gtgcgatcct ggcgctggtt ttaggtgccg gtctggcgga gcgcgtcggc
    22561 ttactgccag cgctaatggt taaaatggca tcgcatgtta atgcccgcta cgccagttat
    22621 atggtgctgt ttattgcttt tttcagccac atttcttccg atgcggcgtt agtgatcatg
    22681 ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc atccagttgc aggtttactg
    22741 gctgccattg caggcgtagg ttgcggcttt acggctaatt tactgattgt cacaaccgac
    22801 gtgttgctgt cggggatcag cacggaagcg gcggctgcgt tcaatccgca aatgcacgtc
    22861 agtgtaattg ataactggta ttttatggcc agctccgtag tcgtactgac gattgttggc
    22921 ggcctgataa ccgacaaaat catcgagcca cggttaggtc aatggcaggg aaacagcgat
    22981 gagaaactgc agacattgac cgaaagtcag cgttttggtt tacgcatagc aggtgtcgta
    23041 tcgctacttt ttattgctgc gattgcgctg atggtgatcc cggaaaacgg gatattgcgc
    23101 gatccgatta atcacaccgt gatgccatca ccctttatta aaggtatcgt gccactgatc
    23161 attctttttt tctttgttgt ctcgctggct tatggcatcg ctacccgcac aattcgacgt
    23221 caggcggatt taccgcattt aatgattgaa ccgatgaaag agatggcggg atttatcgtg
    23281 atggtttttc ccctcgccca gtttgtcgcc atgtttaact ggagcaacat ggggaaattc
    23341 atcgccgtgg ggctgaccga tatactggaa agttcagggc ttagcggcat cccggcgttt
    23401 gtcggtctgg cgttgctttc ctctttctta tgcatgttta tcgccagcgg ttccgcaatc
    23461 tggtcgattc tggcccccat tttcgtacca atgtttatgc tacttggctt tcacccggca
    23521 tttgcgcaaa tcctctttcg tattgccgac tcatccgtat tgcctttagc gccagtatct
    23581 ccttttgttc cactgtttct tggattcctg caacgctaca aaccagacgc gaaactgggt
    23641 acttactatt cgttagtctt gccctatccg cttatctttt tggtggtatg gctgctaatg
    23701 ttgctggcgt ggtatcttgt gggcctgccg ataggtccgg gtatttaccc acgtttgt
//
LOCUS       DAZGCQ010000077        23572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000077 DAZGCQ010000000
VERSION     DAZGCQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.077"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..1587)
                     /gene="amn"
                     /locus_tag="ACHK07_003374"
     CDS             complement(133..1587)
                     /gene="amn"
                     /locus_tag="ACHK07_003374"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO8103663.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGGTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1689..3005)
                     /gene="shiA"
                     /locus_tag="ACHK07_003375"
     CDS             complement(1689..3005)
                     /gene="shiA"
                     /locus_tag="ACHK07_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO8103664.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3320..4372
                     /locus_tag="ACHK07_003376"
     CDS             3320..4372
                     /locus_tag="ACHK07_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO8103665.1"
                     /translation="MFLASLLRHIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLLIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4634..12637)
                     /locus_tag="ACHK07_003377"
     CDS             complement(4634..12637)
                     /locus_tag="ACHK07_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8103666.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALKAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSSATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGIAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNNDTKMVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDNVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTAVPDRIIAGTPQNSSGSVITATVVDNNGFPVKGVTVSFTSRTKSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSRETGARPDTIEASLENGSSTLSTSIQVDADASTAHLTSLYTLY
                     DTQLAGDDTTLYITVNDNYGNGVPLHQVTLSVSPSEGVTLSNNGINTTNHDGYLYASM
                     TATKAGVYQVTATLDNGDSMQHTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTKLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNSYGVSDTKPVT
                     LIADAGTAKLAGFTASSGSFTASTTEGATLTASVTDAYGNPLEGIKVNFRGPATTLSN
                     TSVETDAQGKAEILVTSTIAGTKVVTANLAIAPTEAAMRTLTVKADIDSATITSLEMP
                     EGQVIIREPIAVKAHVDDQFGNPVADQLVTFSAEPSSFNMVISQDTVSTNRQGIAEVT
                     MTPGRYGSYTVKASLANGSFYEKDLVVIDLRLTLTSSSQLIGVNDPSGATLTVRLTHA
                     NGAPLSHELVTFSVTPEGATLSSQTATTNTSGEAQVVLTSNKVGTYAVTASIHSGVII
                     ETQTTVKVTGNPSTAHVASFIADPSTIPANNSDISTLKATVEDGSGNLVEGVNVNFVL
                     KSGSATLTSLTAVTDQNGLATTSVRGAMTGSVTVSAETSYGGAQTVDITLVAGPADAS
                     LSVLKNNRSSLKGDFTESAELHLVLHDLSGHPINVSEGMEFVQSGTNVPYVQVSAIDY
                     SKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLNTTIEFISAEARPMTGTVSVNGAT
                     LPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSSTASWVDVDTSGKVTFKNVGDRN
                     AVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLSQAENYCSNQVGNGYTLPRADLL
                     SNGHMRREIGSLYGEWGDMGNYMNEADFYSMVYWSSNSAGAGQQYIVSLETGTQNTYQ
                     THEFFYGACYKQI"
     gene            complement(12949..13024)
                     /locus_tag="ACHK07_003378"
     tRNA            complement(12949..13024)
                     /locus_tag="ACHK07_003378"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12989..12991),aa:Asn,seq:gtt)
     gene            complement(13125..13922)
                     /gene="mtfA"
                     /locus_tag="ACHK07_003379"
     CDS             complement(13125..13922)
                     /gene="mtfA"
                     /locus_tag="ACHK07_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO8103667.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            14016..14105
                     /locus_tag="ACHK07_003380"
     tRNA            14016..14105
                     /locus_tag="ACHK07_003380"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14050..14052,aa:Ser,seq:cga)
     gene            complement(14675..15205)
                     /gene="yodB"
                     /locus_tag="ACHK07_003381"
     CDS             complement(14675..15205)
                     /gene="yodB"
                     /locus_tag="ACHK07_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8103668.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(15548..16198)
                     /gene="zinT"
                     /locus_tag="ACHK07_003382"
     CDS             complement(15548..16198)
                     /gene="zinT"
                     /locus_tag="ACHK07_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO8103669.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(16455..17090)
                     /gene="msrQ"
                     /locus_tag="ACHK07_003383"
     CDS             complement(16455..17090)
                     /gene="msrQ"
                     /locus_tag="ACHK07_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO8103670.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALIGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(17091..18095)
                     /gene="msrP"
                     /locus_tag="ACHK07_003384"
     CDS             complement(17091..18095)
                     /gene="msrP"
                     /locus_tag="ACHK07_003384"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO8103671.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLHSWFKGNDRPPAPAGKPLDFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(18203..18616)
                     /gene="hiuH"
                     /locus_tag="ACHK07_003385"
     CDS             complement(18203..18616)
                     /gene="hiuH"
                     /locus_tag="ACHK07_003385"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO8103672.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            18749..19420
                     /gene="hprR"
                     /locus_tag="ACHK07_003386"
     CDS             18749..19420
                     /gene="hprR"
                     /locus_tag="ACHK07_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO8103673.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            19420..20778
                     /gene="hprS"
                     /locus_tag="ACHK07_003387"
     CDS             19420..20778
                     /gene="hprS"
                     /locus_tag="ACHK07_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO8103674.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEVCF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(20886..21737)
                     /gene="hchA"
                     /locus_tag="ACHK07_003388"
     CDS             complement(20886..21737)
                     /gene="hchA"
                     /locus_tag="ACHK07_003388"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO8103675.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(22330..23517)
                     /gene="ompC"
                     /locus_tag="ACHK07_003389"
     CDS             complement(22330..23517)
                     /gene="ompC"
                     /locus_tag="ACHK07_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8103676.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGF
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFF
                     GQVDGLNFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGN
                     HHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
BASE COUNT         6107 a         5387 c         5616 g         6462 t
ORIGIN      
        1 taaaaatcat taagacgata ataaaactcc tatccgccta aggaagcaaa cagcctgctt
       61 tattgcaggc tatttgccag atgtcttact taatccttca catctatttt tgataagaag
      121 gttcagaact tattatcgga acggcggctc attaaaggtt cgtaatttac gtgaatgcag
      181 tcggtcgcct tctgcgcgca gcaaatcgat tgcccgaatg ccaatttgca ggtgttcgga
      241 aatagcgcct tcataaaaac ggttagcctg gccgggaagt ttaatctcgc catgcaacgg
      301 tttatctgaa acacacagta gtgtcccgta tggcacgcgg aaacgatatc cttgcgcggc
      361 aatggttgca ctttccatat caattgctac ggcccggctt aagttaaaac gaagtgcaga
      421 agctgagtaa cgtaattccc agttcctgtc atctgtggtt accacagtgc cagtacgtag
      481 ccgctgtttg acttcctcac cgggcctgcc gctcaccagc ttggtggcgt cataaagcgc
      541 acgttgcact tcagcaatgc ttggaatagg aatatcgggc ggcagaaccg catcaagaac
      601 gtgatcatcg cgtaaataag catgtgcaag tacataatcg ccaatggcct ggctttcacg
      661 taatccgcca cagtgaccaa tcatcaacca gacatccggg cgtagcactg ccagatgatc
      721 gcagatggtt ttagcatttg acggtcccac gccaatattc accagagtaa taccctgacc
      781 atcggcggta attaaatgcc atgctggcat ctggtgtttt ttccatgcaa ggtcggaaat
      841 ggcttcttct ggcgcttcgg tttcggcggt gatccagttc ccgccagcac aagaaagggc
      901 aatgtaggga ctatcaggat cgaggatctg gctgcatccc caacgaacga attcatccac
      961 ataacgtgtg tagttggtaa acaagacgaa cggctgaaaa tgttcaactg gcgtaccggt
     1021 ataatggcgc aaccgcgcga gggaaaaatc gacgcggcgc gcatcaaaat gcgatagcgg
     1081 ggagaattca gttgggtgat aaatgccgtc tgcagtttca tcgccaattt gcgccagttc
     1141 tgttgtcggg aagtagcgag ttaacccagc gctcattgag cgatcaagtg tcaattcaga
     1201 gccatcgatg acataaggat aagggatttc atgctgcgag ggttgcaccg agatatgcgc
     1261 accataatcc tgatacagca acgtaagttg ttcattaaga tacgaacgaa agagagtagg
     1321 gcgagtaatc gtggtggtgt agctgcctgc gtgagtaaaa cgaccaaatg cgcgcgtttt
     1381 ggggggattg gttgtgccac cgtcccaggt tacggtcagt gatggataga caaaaagacc
     1441 ttgtttgcgg gcgttttcat caggtaattc gccacttcta atatatttgc caatggcgtt
     1501 gcgtaatgcg actacagatt gctcatacag cgcgtcgagt ttatccagtg cctgagctgg
     1561 ggtcagaccg gagcccttat tattcatgtg tgtctcctgt tccatacaat taccgcacat
     1621 ccgatagtat gtcacaggaa tgtgaaacaa aagtgtaagg cgaagatcgg atgatcttcg
     1681 ccagactatc aagcgcgttg attgtctttc atcaacaaag cggtcattgc ggaaattaga
     1741 catccagcca gcaaataaat ggcgacgcta tgccagttcc cggcaaagta agtgatgagc
     1801 gcagcggcaa taaaaggtgt aaatccaccg ccaaccacac tggcaacctg ataaccgact
     1861 ccagcaccac tatagcgata actggcacca aacatttcgg taaacatcgg ttgttgcaca
     1921 cacaccacca tgtcatgcgc aatgtttgcc agcattatgg agaagaaaac tatccagaaa
     1981 atagattgtg cttcaagcgc cataaagaaa ggaaatgcgc tcaacgttcc gatcaacgcg
     2041 cctgtgatat aaaccctgcg gcgaccaaaa cgatcggcaa gccaggcaaa acagggaatt
     2101 gtcaggcagc ttaatccacc taccagcaaa ccaatattaa ggaaaagttc gcgcggtaac
     2161 cccatattct gggttgaata attaagtgca aaggcggtaa cgatgtacat cgtcagcaac
     2221 tcgcacagtc gtagcgcaat aatcttcagg aaagcaccgg gatgtcgtaa cagagcttcg
     2281 ataaccggga tgcgtttttt cgcggcagct tgataatgtt gctgttgttc aaattccgcg
     2341 gactcctcca tgccattgcg cacccacaat gctcccagta ccagtacgat gctaaacagg
     2401 aaaggaatac gccagcccca gcttaaaaac tgttcgtcag tcgtcatcat actgatcaat
     2461 gaaaccagtc cggttgaaag cagtaaacct acaccgtagc caacttgtac accgctactg
     2521 taaaaggctt ttttattttt tggtgcactt tcaacggaaa gcaacgccgc gcctccccat
     2581 tcgccgccga cggcaaatcc ctgaatggca cgcagcgtca ccagtaaaat aggtgcccac
     2641 cacccaatgg tcgagaatga aggaagaata ccaatcaagg ctgtcgcgat acccatcatc
     2701 cagacggtca gcattaacat gcgcttacgt cccagtcggt cgccaaagtg accgaaaatg
     2761 acaccgccga gcggacggaa aaggaatccg acgccaaagg tagcaaatgc ggcgagcgtt
     2821 cccatcgccg ggcttacttg cgggaaaaac tcgcgattaa acaccagtgc ggcggtgatg
     2881 ccatagagta aaaaatcata ccagtcgacg acggcaccag cgaaactgcc taacgcagct
     2941 cgtcgggcgc gacttaacga aagcgtccct tcatcgggac gggtggagat gagcgtggag
     3001 tccataatga tcctgtttgt actgtctttt tataattagt atggaaaagg taactcacat
     3061 tgccgtcgat ctataaacga accttatgag actacctcga cagagcgcat ctgcaaatga
     3121 ttttatggct gattagcggc aacatgaaaa attcgctgga agatgaaagc gcgggagttg
     3181 ctggtgttgt ttaaggatta agcagaggac ccacaggttc gagatatgaa acataaaagt
     3241 ccgcatatgc acaaaatggc accctgatga gtctgataga ctgcattgca ttataaccag
     3301 tagggaggcg aggttaggta tgtttttagc atcattgttg agacatattg catttagtta
     3361 ctacgattat aaagcttata atttcaatat tgaaaaaaca gattttgttg tcatccatat
     3421 tcccgatcag attggcgatg ctatggccat ctttcctgtt attcgggcgc ttgaattgca
     3481 taaaattaag catctactaa ttgttacgtc gacaattaat ttagaagtct ttaatgcgct
     3541 caaacttgaa cagactaaat taacattagt cacaatgact atgcaggatc acgcaacatt
     3601 aaaagaaata aaagatttag cgaagaacat aacacagcaa tacggtacgc cagatctttg
     3661 tatagaggcg atgcgtaaaa agaacctgaa aacgatgtta tttatcagtc agttgaaagc
     3721 aaaaacgaat tttcaggttg ttggtttaac catgaagtgc tattcccctg tgtgtaagaa
     3781 cgcgtcccgg atggatcaga atctccgggc tcccgtaccc atgacatggg catttatgat
     3841 gcgtgaggcg ggttttccag cagtcaggcc aatatatgaa ttgccactaa gtgaggatgt
     3901 actcgatgag gtgcgcgagg aaatgcgatc gttaggatct tacattgcgc ttaatttaga
     3961 aggtagctcg caggaacgta cattttcatt attgattgca gaaaatctaa tagcaaaaat
     4021 tcaaagtgaa acagatatac caatagtgat cgttcatgga ccaaaagggg aagataaagc
     4081 cagggtatta gtggattgtt ataataatgt ctaccgttta tccttatcac cctcgattaa
     4141 acgttcagca gcaatcataa aagatgctta tatcgcaata actcctgaca cctcaatatt
     4201 acatatggca agtgcctata atacccccgt tgttgcaatt tatgctgatt acaaaacgcg
     4261 atggcccgca atggccgatg tttcggagtc agtcgtcgtt gggcaaaaaa ttgacaatat
     4321 aagtctggat gaattcgcaa aggcattaaa aagtgttttg gcgagaattt gaggcagtac
     4381 gtatgcatag taatgccgat ctgttatgaa cggtggtgtc atcaatactc tgaggctggt
     4441 caatcatcac agaaaaccag cctttggatc aaagctgtac tcaccggata cgatcatagt
     4501 cacaccgaac gcgaatctgg cggtatcgca tttttattta ttcattttcg gtgagagaag
     4561 agcaagctct atagagggaa tgtaaaaaag agtgccccga atggagcact ctttaagaaa
     4621 ttataacgaa aaattaaatc tgtttataac acgccccata aaaaaattca tgcgtttgat
     4681 aagtgttttg cgtccctgtt tccagggaga caatgtattg ttggcctgcg cctgcagaat
     4741 tagatgacca atataccatt gaataaaaat cagcttcgtt catgtaattt cccatatctc
     4801 cccattcacc atacagtgac cctatttcgc gccgcatgtg tccattgctt aaaaggtccg
     4861 cgcggggaag ggtgtagcca ttaccgacct ggttgctgca gtagttttca gcctggctta
     4921 attgcatgag gttattaccg tgattcaccc accagccctt cacccgtact tgcgtttggt
     4981 aaatagcgcc acctgatctt ggggtcgccg tgattattac agcatttcta tctccgacat
     5041 ttttgaatgt caccttaccg gaagtatcaa catcaaccca ggaggcagtg cttgaaaacg
     5101 cataatcagc cgccgttttt cctggtgcaa agttgtcatt attcaactga taatacgccc
     5161 cggtaaatcc ctgcgaaggg aatgaagctg ccggtagggt cgcaccattg actgacactg
     5221 taccggtcat tggtctggct tccgcgctaa tgaattctat cgtggtattc agacctgcct
     5281 ggtgaacacc attcaacacg gggatcagcg ttgcgatacc ctctccgccg cctgtaacag
     5341 tggctttgta ctcgccggag aaatttttgc tgtagtcaat tgcactaact tgcacatagg
     5401 gcacgttggt acctgattgc acaaattcca tcccttcaga aacattgatc ggatggcctg
     5461 atagatcgtg cagaacaaga tgtagttcag cactttcggt aaagtcccct ttcagtgatg
     5521 accggttgtt cttaaggacg gacagcgaag cgtctgccgg gcctgccacc agcgttatat
     5581 ctactgtttg cgctccacca tagctcgttt ctgcgcttac cgtgacgctc cccgtcatag
     5641 ctcctctaac gctcgttgtc gcgagtccgt tttgatcggt caccgctgtt aatgacgtca
     5701 atgtggcaga gccgcttttt aagacgaagt ttacattgac cccttcgacc aggttaccac
     5761 tgccatcctc aacagtagcc tttagcgtgc tgatatcact attgttggcc gggatagtcg
     5821 acggatcagc gataaaacta gcaacatgtg cggtgcttgg gttgccagta accttcacgg
     5881 ttgtctgtgt ctcaataatt acgccgctat ggatcgatgc tgtcaccgca tatgtaccga
     5941 ctttattgct ggtcagaacg acttgtgcct cacctgaagt gttggttgtc gctgtttggc
     6001 tacttaacgt tgcaccttcg ggcgttacgc taaaggtaac gagttcatgg ctcaatggag
     6061 ccccgtttgc atgagttaac cttacagtca gagttgctcc cgatgggtca ttgacgccga
     6121 taagttgact tgatgacgtg agtgttagtc ttagatcgat taccaccagg tccttctcat
     6181 aaaaggagcc attcgccagg gatgctttca cagtatacga accgtatctt ccaggcgtca
     6241 tggtgacctc agctataccc tgcctattcg tagaaaccgt atcctgacta atgaccatgt
     6301 taaaacttga cggctctgca ctgaatgtca caagttggtc agcaactgga ttgccaaact
     6361 ggtcgtcgac gtgtgcttta accgctatgg gttctctgat tattacctga ccttcaggca
     6421 tttccagact ggtgatcgtc gcagaatcaa tatctgcttt gacagtcagc gttcgcatgg
     6481 cggcttcggt aggtgcgatt gccaaattag ctgtaactac tttcgttcca gcaatggtgc
     6541 ttgtcacaag gatttctgcc ttaccctgag catccgtttc gacactggtg ttactcagcg
     6601 tggtggcggg gccgcggaaa ttgaccttga taccttctag cgggttgccg taagcgtcag
     6661 tgacgcttgc cgtcagggtc gcgccctcgg ttgtgctcgc ggtaaatgag cccgatgatg
     6721 cggtaaaacc agccagttta gcggtaccag catcggcaat caaagtcacc ggcttcgtat
     6781 cactgacacc gtaactgttc acgctaactg tcacgggata ggtgccggat tttgtaccgc
     6841 tcagtgtaac ttcagccttg ccgttgatgt ctgtaatggc ggaaccgcct tgtccgagag
     6901 tgaagcttgc gctcacatct gccggtagtg tgaatgttac cgcctcattg gctaacgggt
     6961 tgccgtttcc atcagtcact gttgcctgaa gtttggtcat cccaatgtta ttggcgatga
     7021 aattgtcctc ggtaacgtta agattaacct gcgcagtgag cgtatccgca gtaaagttca
     7081 ccaccagttg tccgctctta ccgccagcca tcgatgctgt aacagtatga gcgcctgctt
     7141 ttgtaccttt cagcgtgact ttcgctttgc cttcagtatc agtaatcgct ttaccgccat
     7201 cgctcagtgt gaagttcgcc ctcacatctt ccggcagagt aaatgttacc tcagtgttgg
     7261 ctatcgcatt gccctctgta tcagcgactg ttgctgttag tgtcgtgaga tcgttattgt
     7321 cggcaatcac cggatccttc gaggctgcca gcgtgatttc agcattcgcg acgttcggca
     7381 cataggtcac tgtgtgttgc atcgaatcgc cattgtcgag ggttgccgtc acttgataaa
     7441 ccccggcttt tgtagcggtc atgcttgcgt ataaatagcc gtcgtggttg gtagtattta
     7501 taccgttatt actgagggtc acgccttcac tcggcgaaac gctgagggtt acctgatgca
     7561 ggggtacacc attgccgtaa ttatcattca ccgtaatgta cagagtggta tcgtcgcctg
     7621 ccaattgtgt gtcataaagt gtatatagcg aggtgagatg tgccgtagac gcatcagcgt
     7681 cgacttgaat ggacgtgcta agcgtggagc taccattttc caggctagct tcaatggtat
     7741 ccggtctcgc tcctgtttct ctcgaggagc gggtattggt ataagtgacg gtagctttac
     7801 cctgttcgtt cgtcacggct tggccgccgt tcgtcatttc ggcagatttt gttctgctgg
     7861 taaagctcac agtcacacct tttaccggaa agccattatt atcaacgact gtggcggtaa
     7921 tgacgctgcc ggagctgttc tgcggggtac cggcgattat gcggtctggc acagccgtca
     7981 actcgataat ttttgccgcc gctgtgtcgc caataaaatg cacagttttg ttgtcgctgg
     8041 caccattatt agccagtgat gcagtgaccg tctgctcacc aaaggcaacg cctgcgagag
     8101 tggcctgcgc gatgccagac tcattggtat tactcactcc cggggtcagg gtgagtcctg
     8161 aagaggctga gctgaatgtt accggaagat tgttcacctc attgtcgaac tgatctttaa
     8221 ccgttgcagt tagcgttgcg ttatcgacgc cattacctgt gatctcatct tttgacatct
     8281 gcaggacaac ctgagccgag gttttgtccg ccacaaacgt taccggctgc gaatcactgg
     8341 tattgttatt acccagcgtt gcggtaaccg tatgcgtgcc cgctttttta cctttcagcg
     8401 tgacatgcgc ttccccattg gcctgggtaa tggcaatgcc gttattttca agggtaaagt
     8461 ttgctgccac gtcctgtggc atggtgaagt ttaccgttat ccccgctacc ggatgattcg
     8521 atgggtcttt caccgtcgcc gtcagctgca cattgtcgct gttattcgtc accacctgtt
     8581 gagcagggat gttcagggtg acctgcgcgt tgctggcatc gggcgcaata ttgactgtcg
     8641 tcgtatagcc attgccgtta agcagtgtgg cttcgactgt atgaacaccc aaaaccgtgc
     8701 ccttaagggt aacttcagca acaccactgt cgttagtatt gcttgtgctc tgactaagtt
     8761 gagtatctgc cgggttggta ctgaaggtga cctgtagatc ttttactgcg ttatcaaaag
     8821 gatctttcac tgttgctgtc agagtcgtct catccacgcc attcccaatg atttccgctt
     8881 tcgatgtttg cagaacgaca actgcgctgt ccttatccgc cacgaatgtg actggttgcg
     8941 catcgctggc attattgtta cccagcgttg cggtaaccgt atgcgtgccc gcttttttgc
     9001 ctttgagggt gacgtgcgct tcgccattgg cctgagtgat ggcaataccg ttattttcaa
     9061 gggtaaagtt tgccgccacg tcctgtggca tggtgaagtt caccgttatt cccgccaccg
     9121 gatgattcga cggatctttc accgtcgccg tcagctgcac gctgtcgctg ttattcgtca
     9181 ccacctgttg agccgggatg ttcagggtga ccagcgcgtt gctggcatcg ggcgcaatat
     9241 tgaccatctt cgtatcgttg ttgccgttag gcagtgtggc ttcggctgtg taaaccccca
     9301 aaaccgtgcc cttaagggta acttcagcaa taccgttctc attagtatta cgcgcgttca
     9361 gactcagttg cgtgtctgcg ggggaggtgc ggaagactac tgacagattt ttaaccacgt
     9421 tatcaaaagg atctttcact gttgctgtca gagtcgtctc atccacgcca ttcccaatga
     9481 tttccgcttt cgatgtttgc agaacgacaa ccgctctgtc tttatccgcc acaaacgtca
     9541 ttggctgtgt atcgctgaca ttgctgttag ccagacgagc cgtgatcata tgcgtgcccg
     9601 ctaacgtgcc ggtcagggag gcgattgcaa tcccgttgct atccgtcatg ccttttccgc
     9661 tgttgctaat cgagaaccga cttgcgacgt cgtttggcac agagaaagtg atttctttat
     9721 cttttagtgg gttgccattt ttatcctgca aggttgccgt cagctgttga ggcgctgtgt
     9781 cggtgacggt gatctcgcct gggggcacac tgagggtcag ggctgcagta ctttgatcac
     9841 cgataaaaat cacctgttga ttagcctgag aaccagagct cacagaggcc gtaaccgtat
     9901 aatcaccatt tttcaaactg gtcagcgtag ctgtggcgat cccgtcgtgg ctattcactt
     9961 cggtttggct cagtttcgct gctgctgaat taacattgaa ggtgaccttg acgtcattga
    10021 gcaggttgcc ttttgcatcc cggactgtcg cggtcattgt ggcgctgtca ttgccgtcag
    10081 cgaccacttc atttttcgac ttctgcagat caacctgcgc ggtactcgag tcgccgacaa
    10141 aactgacaat taatgtttgt ttcacgccat tttcaagggt gacttcaacc gtgttgtctt
    10201 cctgcttact acttttcaga tcaaaagtcg ccagaccatt aacatccgtt tttgcggtgt
    10261 tttgattgtt aaaggaagtt gccgatccgc ttaataccgc aaacgtgacg gtatgatcat
    10321 tgattgggtt gcttccttca tcagcgacat tgaccgagac ggtgtttgct gcattctcat
    10381 tggcgagcac accattattg ctggcagata atgtcgcaat tttcgctgac tgcgggttgg
    10441 cgtcgatgat aaatccagcg gtatgcaaat cttcattcca gttttgcatt aacagcttcg
    10501 cagtaagccc actgcctttg gtataggcgg tataggttgc cttatagacg ccatctgcgg
    10561 tttctttcca gtctgtagtg acaccaggtt tcacattgtc gatgctgact gcggtattca
    10621 gttgctgttt ttgctcctta acaggtttgt cattttcatc tctcagttct accgtcacct
    10681 cgataggatt cccggagaga taacggtcct tatcaatctt aattgacgag tgagtccggg
    10741 atgacgaaac agaaatgata ttcaccacgg cgggggcttt agccgcatcc acaccgttca
    10801 gctgtggcat cagcgtcagc gtgccagaca tcgctcctgt ggtcaggact tgggtatagc
    10861 ttccgtcacc attatctttc cagtccgaca gggtgatgtc ctgaacacct tcgtgacgcg
    10921 tcgagagcac cagcccgata acaggattac ctgctgcatc atgagcaata aaagtcagtg
    10981 tggcgcttga atgggaatcc gcgctcaacg tctgggtact taacgatacc gaggaatctt
    11041 tctggcttag cgtaggtgcc tgaacgacta ccatgctctg ttcacgattc gaaaaattgc
    11101 ctttgacatc ttcagcggtg acttcaatcg gccaggtgtt atcggtttct ggcgtactgg
    11161 tgaaccggta cgccggcagg gtaaccagaa tatctttacc cgttgtaacc actttaccac
    11221 cggcagcttt cagagcggtg gcttcgacgt tatagccttt cagggcatat ttggtttgta
    11281 gcgacgaaac cagtgatttc acttctcctg acttccctgt cacggggtct gtcagggtca
    11341 ggcggaccag ttcttttttg cgatattcca gaacgatgtt gttgttgcga tccaccagat
    11401 cataacggct gcctgcaagg ctacgccgtg cagcgacttc attcgggtca agctgtttct
    11461 gcattgcgct gccaggttgc caggtaaaat cgacggcaaa tcgggtgtca ttttcgccct
    11521 gtttaccctg gcgttgctcc gcgctaaagg tcatcagcgg gaagggggta tagtttagtc
    11581 cagcggttat ggcatgagga ttactttgcc gatcgtcttt atcgaacagg gccacttcat
    11641 cgccataata ctgttcatag accagtttac cgccaaggtg cggccaggcg ggtaaccagc
    11701 cttctgcgcg tacatcccag ccattggccg ggcgtgcttc ataatcgttg tccagttcag
    11761 gtgcgctgcg ccagtttgtc agtcgcaaat agccgttact gcttaattta agatagtcgc
    11821 gccagtactc cgcgccaatg ccggcgcggg agtggtaacg gctaagatcg tggtcgaaaa
    11881 agaagttgat gcccgacatc catgtgggag tgaaatgacg ccaacccaag ccgttgttaa
    11941 tctgcgtacg ctcgtcagta cgatggagag tatgctgact gaaaaagaga ttatcaggtg
    12001 tttcatacca cggatggaga aaatcgaact gggagttctt caggctaaaa tcttcatcca
    12061 cgcccagcgt gattcttgcg gtaccgaagc ggcttaacca gtctgtcatt gcgcctgaag
    12121 cctgagaaga ggcccatcca cgcgccatat ttgccgcttg ctcgctgttc atatcttcgg
    12181 cgagcagaga tccgatttgc tgtgaagtac tggctatctg ttgctcaagg ttgccactgc
    12241 tattacccgg tggcggggtt aaattatttt cactaacttg tgccgggaca tccagttcat
    12301 caccctggcg gacattatca aaacctcgag caaacgtacg aaactggttg agtttgcgta
    12361 actcagccac cgaaataccg aaacgttcgg caacgctttg ggccgattcc agcgctccaa
    12421 gggtgtaggg caccgtattg gcatttgcaa tggcaatttg tgcaggaact ggttgttggg
    12481 ttgcggcgtt taccacacct tgtgctgccg cagccatagg gaacacaagt tgagttacca
    12541 ggcagatacc cgcagttaag cggcgtagtt tgatacccat cccgcataag atttgtcggt
    12601 catttatttc ttctccactt ctcttcttcg tagccatgct taccttccct gaacgagcta
    12661 acataaatgc gccctttcta gcacagcgaa aaaatgtaaa tcctgatagc aatatgattg
    12721 aaaattaata acttttcgtt aggcagtttt gggtgtgagt tgcaagaggg gagactactg
    12781 aattactcaa gttttataat cgaggggaaa atggtgatgg cgccatagca aaacgccctc
    12841 aaccataaag gtcgagggcg cttaagatgt caaaacccgc tatccgttaa aaaacaatgt
    12901 tcaactaagg tcagtgacat tgcgctaaaa aagcgaattt ttagaatttg gctcctctga
    12961 ctggactcga accagtgaca tacggattaa cagtccgccg ttctaccgac tgaactacag
    13021 aggaatcgtg tgaacggggc gcatattact tagcggtacc ttgtctgtca acactaaatt
    13081 tcataagtca tttcaattgg ttaattaatc tgcaaagttg ttaattaatg aacattcgtc
    13141 gccgaaaacg agtctgtatc attagcgtga tgcagtctct gcaaaggatc ttgttgataa
    13201 aattggcaga aacgttgcca caatgaagga aaacgaggag caaaaagttc tggggcgcta
    13261 aagaaatatt cagaaagtac ggcaaaacat tcagcaggat cactggcagc ataagcatca
    13321 atgctcgccg cattctcgcc aaccaattcg atttcttcct gaatgttgtt cattgcagca
    13381 tgaagatcgt gttcccagcc agcaacctca cgcaacggaa taaagggaac tccgctggcg
    13441 cgatcgccgt tacgggtgtc cagcttatga gcgacttcat gaataatcag gttaaaacca
    13501 gaagcatcaa aagaatcttg tatatccaac cagttcaaaa cgataggccc ttgctgccag
    13561 ctctgacctg actgaacaat acgttggtta tgcaccagac cgatatcgtc ttcccattca
    13621 tcatcgacca caaatggcgc aggataaatt aagacttcat gaaaaccatc cagccattcc
    13681 agtcctaact ccagaacggg taggcaaaat agaagtgcta tccggcagct tcttaatgaa
    13741 tccagttcaa agccctgtaa aggaacaagc cgcttttgct gtaaaaaacg ttcggcaaga
    13801 gtgactaatt tgctttgttc ctgttccgtc agacacgtta aaagggggat cgatagtgct
    13861 tcctgccagg gaagggcagt ttgatgtgct gattcttgta ctttccaggg ccacttaatc
    13921 atcgttttgc tcgcaaactc gtcacttgaa caaaattgca cggacaggga ctgttaaaat
    13981 gccaaatttc ctggcatcat ggcaaccatc tgaacggaga gatgccggag cggctgaacg
    14041 gaccggtctc gaaaaccgga gtaggggcaa ctctaccggg ggttcaaatc cccctctctc
    14101 cgccacttta tcaatgactt atctcccgac ttcccgcctt gcttttccta aacagaacaa
    14161 tcgtagaata ttcttgaagg gttagatcgt cactgttttc tgttcgatac tgtgacattc
    14221 agcacttgat tcgctatgga tctgacagga agctttcgag cgaaaatgtg cagttattca
    14281 gtcgttttct tatcgctcac cattattctt ttagacattc atcctatgaa actgccgcaa
    14341 agttggtggt gggaactgaa gttgcgtaga gaaggggtca ataattgagt caaaaaagga
    14401 acgcggccgt cagattaaag ccgcgggaca aagtccataa tttccaagag ttgttgaatt
    14461 tcccagcttt tttgaaagcc tttgtatcat actaacaagt aaaaaaggtt taaataccaa
    14521 aattaacctg tatttttgat aagtcttacc attgtacgat ttacacagtt tctgctcctc
    14581 cttgttgata gtggaaaggg tgcattgtgc acttgcgtaa acatttatga cgcgttgatg
    14641 tccatggttc acatttcctt gtcaggggat cgacttattt gcggcgaggc atcattcgta
    14701 acagagtatt atctttctga atgtagtgat gaaagagtgc tgcgccagcg tgagctgcga
    14761 ttaaaaagta acctatattt gcccaggttt cgtgaatatt ttttatcagt gctttaattt
    14821 cgctgtttgg ggtaacaaag ggagacacat tgaaaccaag gaaactccac gattttccac
    14881 tgtaagccat taaagcaatc cccagcagag gaagcgcaag gaaggttata taaagcatga
    14941 tgtgcattag cgaagccgct ttcatttgcc aggcaggtgg cggtggcaca atagaaggat
    15001 cactataacg gtgttttata atcaggcgtg aaaacattaa cacccaaacg aatatacccg
    15061 cattgtaatg tgtttctcgc atcagcagat aagtactact acctttagga aaccagccac
    15121 ggagttccat cgcggcatag gttattgcaa cgaaaagcag cgttatccaa tgtaaataaa
    15181 tttgagtttt tgagaatcgg ttcatttttg tagctcctgt gattttcaca gtcaagctat
    15241 cccacgaacc ttaaaaattc cttatacaga tgtggtttta agaatttgtt atttaaaaaa
    15301 ttgatagtgc acattgtaac cagccaatat aagcaatggc gtgagcacag gtatctttct
    15361 ctgttggccg tattgtgtat ggaatccgtt attggttaac tcaatgcggg ttagcttatt
    15421 aatatacaga cacgtcatag tgatgagcgt gtctgtagct agcttgcgtt cagtggcgtt
    15481 tgtggatgga ccagtggcag ttatgatgac ttactaaagc ggcatcgagg cgttatcatg
    15541 agaatactca atgagacatc atttcctcga ccacttcttc gctactcaac tgatatggat
    15601 aatacgttgg ccagttttcc atttcattta ataaagattg ctgtgaatca ttacccataa
    15661 aaatgtgaaa atgagatgat tttcgtggtg caattatatg atcgctaaat tgtatatatt
    15721 taggggcttt gctttcagga tctttacatt cgaataagta acgaacgcct ttcttgcctg
    15781 atttataggt gagtattttg tatccatcgt aatcatattt acaggatgtt gtttcattat
    15841 ttctatggaa ttcaacaatg ccgtcctcaa tgccaatcat ctcgatatct gttgcataac
    15901 ctttgtgata ataatcttta atttcagcaa atgttttagt tttatctgca tccgctttct
    15961 tctgaaagac ggggtcaagt ttgccacttt gcagtaaagg ataaacggat tgccagactc
    16021 catcccagtc actgagcgtt cggttttgta cattggcatc atcaaaaaca ccattagcag
    16081 ctttttgttc gacctctgtt aagggtttgc cgtgtgagtg atgaccatgc gaaaaggcag
    16141 gagcgctaac aataaagaca cctaaagcaa cagccagttt gtgaagacga atcgccaaaa
    16201 cagttttctc cagagtcatt gtatatgatg tgtaatgtta tattgtaaca tatgacaaaa
    16261 tgcaacaact cttaggagat gagagcatag ttttctcagg tgtacttatc tatcttattg
    16321 aaataaaatg aattgtgttg ataatttgag gttgatgaca aacgaaacat aacctgatgc
    16381 gatctgcgta ggccggatag ggcgctcgcg ccacatccgg cagggataac gcgtactttg
    16441 tcggcaatat gaagttatac agataatttg ttgtgaacct gcttgcgtaa ccggttaaaa
    16501 agggaacgca acttcttata ccgtaaggct aaaagcagta cagccagccc agcgtagatg
    16561 agcggctgcg gtgaaataat cttcaccgac cacagataat gtattggggc caggatcgcg
    16621 acaagataga cgaaattatg caactgttgc cagtgcttgc cgagttttcg ctgcatcgcc
    16681 tgggttgaag taaacgctaa agcaagcaaa ataacccagc tgataattcc taacgttaaa
    16741 taaggccggg taattaactc cttgcctatt aacgccagat tattcacgcc cagctccagc
    16801 aatgcgtaac tggttaaatg cagtgtcgcc caggcaaagc accataagcc taacaggcgg
    16861 cgagtgcgta tcaataacgg ctgttttgcg tagcgtgcca gaggtgtgat taacaaggtc
    16921 gccagcaaaa atttcagtgc agtgcgaccg gtaaaatgct gaatatcttt cactggatcg
    16981 gcacccagtc caccgtgatt gatcgcccag accagccaga gaaacggcaa caatccggca
    17041 agatgcaggc aaactttcag ccatgtcacc tgttttgctg tcagacgcat ttagaagttc
    17101 tcccgcagat ccaggccacg atacagcgat gccacctggt cggcgtaacc attaaacagt
    17161 agcgttggct ggcgctgtac atcgaggatg ccgcctgaac caataaatcg ttcggtagcc
    17221 tgtgaccagc gtgggtgatc aacatgcgga ttaacgttgg cgtaaaaacc gtattcgtca
    17281 ggcgctgcca gattccaggt ggttggcgga cgctcgcggg tcagcttaat actgacgatc
    17341 gatttaatcc ctttaaagcc atatttccac ggcacaatca gtcgcaccgg cgcgccattt
    17401 tgtggcggta acgccttgcc ataaacacct acggtcatca gtgtgagcgg atgcattgct
    17461 tcgtcgagac gcaatccttc gacataagga tatttcagcc cgccgccgat aaagcggtcc
    17521 tgctggcctg gcatctgttc cggtgcataa attgtttcga aagcgacata cttcgcattg
    17581 ctggtgggtt cggcaagcgc cagcaatttg tgcagtggaa aaccaatcca cggcaccacc
    17641 atcgaccatg cttctacgca gcgcatacga taaatacgct cttccagcgg gaagcgacgg
    17701 gttaagtcat catgatcgag ggtcaatggt tttgccactt cgccgctgat tttcagtgtc
    17761 catggatcgg ttttcaggct accagcatta gcggcgggat cggctttatc cagcccgaat
    17821 tcatagaagt tgttataacc ggagacttta tctgctggcg tcagtggcag gttattttgc
    17881 caggcggcag gcttgctgaa gtccagcggt tttccggcgg gggcgggcgg acgatcgttc
    17941 cctttaaacc agctatgcag atcggcatgt gcagcgtggg gcaaagaaag tgcagctgcg
    18001 ctgatgccca gtgcttttaa cacctgccga cgcttcataa agaataccga ctcggccgta
    18061 acatctgatt cttttaaaaa ttgattcttt ttcataagcc atcacacttt ttgtcgttag
    18121 ccagcaagag ccgtttactg ttaatctgtc agggagtgtc ctccctaaca gtagcagaga
    18181 tctgctttta catatttatg cattaactgc cacgataggt tgaataccca tattggctaa
    18241 gtaataaagg cacatgataa tgctcgttca ctttattaat atgaaattca acggggatct
    18301 cagggaagaa actttcaaga ttttgtttct tgaaatagtc cccggtttta aatacgacac
    18361 ggtaatcgcc cgtagttgca gtttgctcgg gccacagtgc cttaattcgt ccatccttat
    18421 ctgttttggc agtattaagt tgtaaccagc cgttgtccgc tttcttttca agagtgactg
    18481 tcacgtcggc agcgggtttt cctgtttgct ggttcaaaat gtgcacgcta agaatgtttt
    18541 gttgtgcagc atagaccaaa gaaggtaatg aaaatgcagc cgttactacg gagagtacta
    18601 aataacgctt taacatgttt atatccttgt catgtgaatg agttttgagg atagcgaaaa
    18661 agcgtccagg gtaaccttac agcagcatta caattttgta atgaaccagg ctgtttctat
    18721 aacatatgat ttatggcata ttattttcat gaagatttta cttattgaag ataatcaaag
    18781 gacccaagag tgggtaacgc aggggctttc cgaagcgggt tatgtcattg atgccgtttc
    18841 tgatggcaga gatgggcttt atcttgcgct gaaggatgat tatgcattga tcattctgga
    18901 tattatgctt ccgggtatgg atggctggca gatcttacaa acgctaagaa cagcaaagca
    18961 aacccctgtt atttgcctta ctgcaaggga ttctgtcgat gacagagtca gagggctgga
    19021 cagtggggca aatgattatc tggtaaaacc tttttcattt tctgagttgc tggcaagggt
    19081 tcgggcacaa ttaagacaac atcacgcttt gaattcaaca ttagaaatca gcggcttaag
    19141 aatggactct gttagtcaaa gtgtgagcag ggacaatatc agtattacac tgacgcgcaa
    19201 ggagtttcag ttactttggc tgctggcctc cagagctggc gagattatcc ccagaacggt
    19261 tattgcgagt gaaatttggg gaatcaactt tgatagtgat accaatacgg tggatgtcgc
    19321 cattcgcagg ctccgcgcaa aagttgatga tccttttcct gaaaagctaa ttgccacaat
    19381 ccgtgggatg ggatattcgt tcgtagcggt aaaaaaataa tgaaaagatt atctataacc
    19441 gtccgtttaa ccttgctttt tatattgctg ctgtctgttg ctggcgccgg aattgtctgg
    19501 actctctata atggcctggc aagtgagttg aaatggcgcg atgatacaac actcattaac
    19561 cggacagcac agatcaagca gttgttaatt gatggggtaa atccagatac gttacctgta
    19621 tactttaacc ggatgatgga tgttagtcag gatatcttga tcattcatgg tgatggcatc
    19681 aataaaattg ttaaccggac aaatgtcagt gatgacatgt taaataacat acctgctagt
    19741 gagacaatca gcgcagctgg catttacaga agcattatta atgacactga aattgatgct
    19801 ttacgaatta atattgatga agtttcgcca tcattaacgg ttactgtggc taaattggct
    19861 tcagccagac ataacatgct tgaacagtat aaaattaata gcattataat ttgcattgtc
    19921 gccattgtac tttgctcagt attaagtccg ctgttaatca gaacgggatt acgagagatc
    19981 aaaaagttga gtggtgtaac ggaagcgctg aattataacg atagccggga gcctgttgag
    20041 gttagcgcat taccgagaga actaaaacct cttgggcagg cgttgaataa aatgcatcat
    20101 gctttagtca aagattttga gcgtctaagt cagtttgctg acgatctcgc tcatgaactt
    20161 agaacgccaa ttaatgcatt actgggtcag aatcaggtta cgctaagtca aaccagaagt
    20221 atcgctgaat atcaaaaaac aattgccgga aacattgaag agctggaaaa tatttcgcgg
    20281 ttaacagaga acatactgtt tcttgccagg gcagataaaa acaatgtttt ggtgaaactg
    20341 gactcgcttt ctctcaataa ggaagtcgaa aatttgttgg actatcttga atatctttca
    20401 gacgagaaag aggtttgctt taatgtcgag tgcaatcagc aaatctttgc ggataaaatt
    20461 ttactgcaac gaatgttatc gaatcttatt gttaacgcta ttcgatattc gccagaaaaa
    20521 tcgcgtattc atataaccag ttttcttgat accaacagct atcttaatat tgatatcgcc
    20581 agccctggaa cgaaaattaa tgagcctgaa aaactcttcc gtagattttg gcggggagat
    20641 aattcgcgtc attccgtagg tcagggactt ggtctttctt tagtcaaagc gattgccgaa
    20701 ttgcatgggg gaagtgctac gtatcactat ctcaataagc ataatgtgtt ccggattacg
    20761 ttaccgcaaa gaaattaaca tagggcttca gtacgccata aattaaccgt gaaaaaacgc
    20821 agccacgtcg tgagtactaa cgcggctgcg attgattatg cgcttacatt caaacgtaac
    20881 agggattaac ccgcgtaagc tgccagcatt tcctgcgccg ccagtttacc caacgcattc
    20941 gctgcaaaag gactgtcgcc ggtgagaagt ttacggtcct tatgtactcg cccggtgatg
    21001 tcgtcattaa tgatattcat gcccattttc ttcagttctt cgccgaagta ccaggtgaga
    21061 tgacccggca tatagccaat ctctggcgtt tgtttgtctg cggcgtctgg gaatgcgcaa
    21121 atggaataac cattcagtgg gttatcgccg tggcgaagcg ccagaaaagc cgccgggccg
    21181 tggcaaaggg agataacaaa acggtcattt ttgattgccc actgtaaagc ggcagccacg
    21241 tcctggcttt caggtaaacc aataagtgcg ccatgaccac caggaacaaa gattgctgca
    21301 tattcgctat cagcgttgag gctggcgacg acatccgcga gtttcttcgg attgcggaac
    21361 aacgatttat gctgctcaaa gaatggcatt actttttcat ctttgtgcgg catagcccag
    21421 tattcaaact tggtcatcag accggaaatg gtcgccactt cgaattcgaa acctgcagca
    21481 tggagatgat acaacggcag caacgtttca atcggatggt taccggtcga gaacagtttt
    21541 ccgttatcgg tcggcaaata acgttcgtcc gcggcgatca ccagaatttt atgtttaccg
    21601 cgatacggtt ttggatagtc cacgccatca agatcagaga caggactggt atattggcta
    21661 agcgaatatt ctgaagggaa gaatgcatta tcttcagcaa tatcgacctg cggattttta
    21721 cttgtttgaa cagtcatagt gtattcctta ttgttgctta gttagggtag tcactatatt
    21781 tgcgactgag ctggtcgcta tttttaaccc ggctagaaaa ttgagtcaat atatcgaaag
    21841 cggtagtcgg cgggtgtaag tatgtaatga tgatgctgca ctggtgttga atattattca
    21901 cgccaaattt tccttattga gtaacgtcgt cgcaagtggc ctcaatgatt ttagaccatt
    21961 atcagaggta cggggtggcg gggagagatt tagtgctgtg gaatattgtg gtgctgatga
    22021 atatttatat ccaggattgg cagagaaaaa gcttttttca tgcactattt ttgcaaatat
    22081 ttctatatgg gaagtgtgtt tgttgcggat taattcataa agaaatgtat ttggggtgtt
    22141 ggctggaaga gtagctggta gaagatattg tgccaggagg ggcagggtaa ggtggtgtat
    22201 cgatatgctt tggcaattgc gtgtgccgca gagggagatg aaatgtcaat aaaaaagcct
    22261 gagttcgatg agggacgaac tcaggctagt gttcagtttt acagtaacac gccgcagcga
    22321 gttggctgat tagaactggt agactaagcc cacagcaacg atatcatcgg tagcgatgcc
    22381 gttttctttg tagaagtcgt cgtcgttgtc cagcaggttg attttgtaat caacataggt
    22441 ggacatgttt ttgttgaagt agtaggtcat accaatatca acatatttaa ccagatcttt
    22501 gttggtgtag tgatgattac cctggctgtc gaagttatca ctatttacac cgcctaaatc
    22561 cttaccttta gattgcaggt aagcgatgga cgggcgcaga ccaaagtcga actgatactg
    22621 agcaacaact tcaaagtttt gggttttgtt agcgatggca tcagcatcac cgtaaccagt
    22681 catgttacgg gtttcagcat acatagctgc taagtaaacg ttattagcgt cgtatttagc
    22741 accaacggtc catgcttctg ctgtttcacc gccagcagtt gcatcgaagc gatctccata
    22801 acgagtgtta tgagtacctt tgtgaacctg attgtcggta cggtcagagt tagagtaagc
    22861 agcgcccaga cttaaaccaa agtcaaagtc ataggaagtg gacataccgt aaccgtcgcc
    22921 gttctcttta gcaagtttac gtccattacc gttacctgaa ccttcttgat caaataatcc
    22981 gcttttctcg ttgttaccct gatactggag tgcaaagttc agaccatcaa cttgaccaaa
    23041 gaaaccattg ttacgatagg ttgctacgct gttagcacga ccgttcatga agttgtcagc
    23101 gccagcatag gtatctccac caaattctgg cagcatatcg gtccaagctt ctacgtcata
    23161 tacgacaccg tagttacgac catagtcgaa agtaccattc tgaccaaagc cgaaacctgc
    23221 aaatgccaga cgcgttgcgg tattaccttt gtcgctttca ggaccgtttg cgccaatgtt
    23281 aaattcccac tgaccataac cagtgaattg gtcagcgatc tgagtttcac ctttgaaacc
    23341 gatacgggca tatgacatgt cgccatcgct gccagcatca tcagagaagt agtgcaggcc
    23401 cgctactttt ccgtacaaat ccagtttatt accatcttta ttatagactt cagccgcatt
    23461 tgctgcgcca gcaactaata acgccgggac cagcattgcc agaacttttc ttttcattat
    23521 gtattccctt gtgattataa tcttcatgaa tatatcaata agtgccgtta tc
//
LOCUS       DAZGCQ010000078        23294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000078 DAZGCQ010000000
VERSION     DAZGCQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.078"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1145
                     /locus_tag="ACHK07_003390"
     CDS             <1..1145
                     /locus_tag="ACHK07_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8103677.1"
                     /translation="YFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTK
                     SNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHN
                     LSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIA
                     LLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAV
                     HDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSI
                     VDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVP
                     EQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1326..2816
                     /gene="dgcJ"
                     /locus_tag="ACHK07_003391"
     CDS             1326..2816
                     /gene="dgcJ"
                     /locus_tag="ACHK07_003391"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO8103678.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2859..3362
                     /gene="yeaK"
                     /locus_tag="ACHK07_003392"
     CDS             2859..3362
                     /gene="yeaK"
                     /locus_tag="ACHK07_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO8103679.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3363..3467)
                     /gene="yoaI"
                     /locus_tag="ACHK07_003393"
     CDS             complement(3363..3467)
                     /gene="yoaI"
                     /locus_tag="ACHK07_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO8103680.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3637..4083
                     /gene="yeaL"
                     /locus_tag="ACHK07_003394"
     CDS             3637..4083
                     /gene="yeaL"
                     /locus_tag="ACHK07_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO8103681.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4040..4861)
                     /gene="nimR"
                     /locus_tag="ACHK07_003395"
     CDS             complement(4040..4861)
                     /gene="nimR"
                     /locus_tag="ACHK07_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO8103682.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4958..6139
                     /gene="nimT"
                     /locus_tag="ACHK07_003396"
     CDS             4958..6139
                     /gene="nimT"
                     /locus_tag="ACHK07_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO8103683.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6194..6541
                     /gene="yeaO"
                     /locus_tag="ACHK07_003397"
     CDS             6194..6541
                     /gene="yeaO"
                     /locus_tag="ACHK07_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO8103684.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6563..6817)
                     /gene="yoaF"
                     /locus_tag="ACHK07_003398"
     CDS             complement(6563..6817)
                     /gene="yoaF"
                     /locus_tag="ACHK07_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO8103685.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7000..8025
                     /gene="dgcP"
                     /locus_tag="ACHK07_003399"
     CDS             7000..8025
                     /gene="dgcP"
                     /locus_tag="ACHK07_003399"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO8103686.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8058..8156
                     /gene="yoaK"
                     /locus_tag="ACHK07_003400"
     CDS             8058..8156
                     /gene="yoaK"
                     /locus_tag="ACHK07_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO8103687.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8159..8233
                     /gene="yoaJ"
                     /locus_tag="ACHK07_003401"
     CDS             8159..8233
                     /gene="yoaJ"
                     /locus_tag="ACHK07_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO8103688.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8292..8540)
                     /gene="yeaQ"
                     /locus_tag="ACHK07_003402"
     CDS             complement(8292..8540)
                     /gene="yeaQ"
                     /locus_tag="ACHK07_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8103689.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8688..8870)
                     /gene="yoaG"
                     /locus_tag="ACHK07_003403"
     CDS             complement(8688..8870)
                     /gene="yoaG"
                     /locus_tag="ACHK07_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO8103690.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8874..9233)
                     /gene="yeaR"
                     /locus_tag="ACHK07_003404"
     CDS             complement(8874..9233)
                     /gene="yeaR"
                     /locus_tag="ACHK07_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO8103691.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9406..10044)
                     /gene="leuE"
                     /locus_tag="ACHK07_003405"
     CDS             complement(9406..10044)
                     /gene="leuE"
                     /locus_tag="ACHK07_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO8103692.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10171..11094)
                     /gene="dmlR"
                     /locus_tag="ACHK07_003406"
     CDS             complement(10171..11094)
                     /gene="dmlR"
                     /locus_tag="ACHK07_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO8103693.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFATVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQLILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11197..12282
                     /gene="dmlA"
                     /locus_tag="ACHK07_003407"
     CDS             11197..12282
                     /gene="dmlA"
                     /locus_tag="ACHK07_003407"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO8103694.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12533..14143
                     /gene="yeaV"
                     /locus_tag="ACHK07_003408"
     CDS             12533..14143
                     /gene="yeaV"
                     /locus_tag="ACHK07_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO8103695.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICVGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGALLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14175..15299
                     /gene="yeaW"
                     /locus_tag="ACHK07_003409"
     CDS             14175..15299
                     /gene="yeaW"
                     /locus_tag="ACHK07_003409"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO8103696.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15355..16320
                     /gene="yeaX"
                     /locus_tag="ACHK07_003410"
     CDS             15355..16320
                     /gene="yeaX"
                     /locus_tag="ACHK07_003410"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO8103697.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPLDTTHYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKIGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16374..17489)
                     /gene="rnd"
                     /locus_tag="ACHK07_003411"
     CDS             complement(16374..17489)
                     /gene="rnd"
                     /locus_tag="ACHK07_003411"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO8103698.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17571..19256)
                     /gene="fadD"
                     /locus_tag="ACHK07_003412"
     CDS             complement(17571..19256)
                     /gene="fadD"
                     /locus_tag="ACHK07_003412"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO8103699.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19461..20042)
                     /gene="yeaY"
                     /locus_tag="ACHK07_003413"
     CDS             complement(19461..20042)
                     /gene="yeaY"
                     /locus_tag="ACHK07_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO8103700.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20082..20777)
                     /gene="tsaB"
                     /locus_tag="ACHK07_003414"
     CDS             complement(20082..20777)
                     /gene="tsaB"
                     /locus_tag="ACHK07_003414"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO8103701.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLSDIDALAYGRGPGGFTGVRIGIGIAQGLALGAALPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGVWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20835..22745)
                     /gene="yoaA"
                     /locus_tag="ACHK07_003415"
     CDS             complement(20835..22745)
                     /gene="yoaA"
                     /locus_tag="ACHK07_003415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO8103702.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     KLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22877..23221
                     /gene="yoaB"
                     /locus_tag="ACHK07_003416"
     CDS             22877..23221
                     /gene="yoaB"
                     /locus_tag="ACHK07_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8103703.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5714 a         5959 c         5657 g         5964 t
ORIGIN      
        1 aatatttcac taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg
       61 gccttatcta tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac
      121 tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta
      181 tttgtttaac ctcgctggcg ctcttttgtt atggaaaaga caacatcctt gacaacaata
      241 agaaaaaaac tggcattctg ttgctggcgc tgattccttt tttagttttt ccccttctgg
      301 cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgat tactgtccgg
      361 acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat
      421 ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag
      481 cattattatg tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata
      541 attataccat ctggtatatc agtcgcggga ttgaagtctc cagtaaactg tttgttgtgt
      601 cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg
      661 atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt
      721 cgcgacctgt tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca
      781 tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta
      841 tccagcaaag catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct
      901 tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa
      961 atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca
     1021 gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag
     1081 cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata
     1141 tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta
     1201 ctattacccc taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg
     1261 tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat
     1321 ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc
     1381 tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc
     1441 tgcgctatat cgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga
     1501 gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac
     1561 agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga
     1621 ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca
     1681 caattgtgga agctgcagca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc
     1741 accaggatga ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg
     1801 atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgctatga
     1861 ataactggtc cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg
     1921 tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact aaacagaatg
     1981 tgatgagttt gctgacgccg gtgtatgttg cagggcagct aaaagggatt gtgctgctgg
     2041 atattaacaa aaacaatctg cggaatatct tttacactca tgaccgccct ctcctctggc
     2101 gttttctcaa tgtcacgcta accgatacag attcggggcg cgacattatc atcaaccaga
     2161 gcgaagataa tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg
     2221 tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct
     2281 ggattttgac ggcgttaatt ttgctgaata tggtgcgaat gcacttccgt ttataccaaa
     2341 atgtgtcgcg agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa
     2401 ctcctgaact ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta
     2461 ttgctattga catggacaag ttaaagcaaa taaatgacac cctcggtcat caggaggggg
     2521 atttagcgat tacgttatta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg
     2581 ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg
     2641 cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaga
     2701 tcggcttctc ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata
     2761 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     2821 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     2881 gcgtgacgca tcagcggtta atcgctttat tatcacaaga aggtgcagac ttccgcgtcg
     2941 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctctag
     3001 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     3061 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     3121 gattacgcgc ttcgttggcc agcccagcgg aagtcgatga actcacaggc tgtgtcttcg
     3181 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     3241 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     3301 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     3361 agctagcctg tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatggcaaaa
     3421 aacgatgacg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     3481 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     3541 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
     3601 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     3661 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     3721 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     3781 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     3841 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     3901 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     3961 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     4021 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     4081 tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa
     4141 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     4201 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     4261 accccgtctc ttttactatc agtcgggcaa gattgcgttc actcatcgca aaaaagccag
     4321 cccattgccc caatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     4381 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     4441 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     4501 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     4561 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
     4621 tcacttgatt gctatgttcc accccgcccg gtatccaaac cgcatattgc ggcggcacca
     4681 tccacaaagc attttccacc gtacaggtaa ttgcaccatg tagcgccagt atcagctgcc
     4741 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
     4801 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctatgcatca
     4861 ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
     4921 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
     4981 cggcaaaaac aggattgtcc ttatcgctgg cattctaatg attgccacaa cattacgcgt
     5041 cacctttacc ggcgcagcac cgttactgga tacgattcgt tccgcttact cgctgacgac
     5101 ggcgcaaacc ggcttattga ccaccctgcc attattagcc tttgcgctaa tctcacctct
     5161 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
     5221 gatctgtgct ggtatcgcaa ttcgctctct cccttcgcct tacttattat ttggcggtac
     5281 agcggtcatt ggcggtggaa ttgcattagg caatgtctta ctgccaggat taattaaacg
     5341 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
     5401 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
     5461 cgcgttgctc atgctgatgt cttttcctct gctggctctt tttttatggc tgccacagtg
     5521 gcgaagtcaa caacatgcaa atttgagtac ctcacgcgcc ttacatactc ggggtatctg
     5581 gcgttctccg cttgcctggc aggtaacatt gtttcttggg atcaactcac tggtctatta
     5641 cgtgattatt ggctggcttc cagcgatcct catcagtcac ggctatagcg aagcacaggc
     5701 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatcccact
     5761 tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc
     5821 agtgggcgcg gtcgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
     5881 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
     5941 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
     6001 agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc
     6061 actgatgggg gttgcaatac tttcactact gatggcgatt ttcggacttt gcgccgggag
     6121 agacaaagaa attcgctaat atccggtgct atagtgacgt gaccaatcat gcgtggaagg
     6181 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggcag aacagagcga
     6241 tggttatcgc gtactggtcg accgcctctg gccgcgtggt atcaaaaaaa ccgatttagc
     6301 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
     6361 cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga
     6421 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctttactc
     6481 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
     6541 attttagtac agcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
     6601 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgttg
     6661 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
     6721 taccaatctg cggcggttta ggtgcttctg gctgagaaga ggttgaacaa ccagccagaa
     6781 ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc
     6841 aggcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
     6901 aatcatatta cgccttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
     6961 aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc
     7021 cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gcctggtccg acagcttctg
     7081 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
     7141 gcgcgcctgc agcatataat gtttgcccgt aacagccaga aaatgcacat cccggagaat
     7201 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ctgctttttc
     7261 agcgatgaag tccccgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc
     7321 acatttctga gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
     7381 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttgca gttattcgcc
     7441 ggactgattg cacaatatat tcaaaaagag gcgctggtag aacagctgcg cgaagccaat
     7501 gccgcgctga tagcgcaatc gtataccgac tcgttaaccg ggttaccaaa tcggcgggcg
     7561 atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca taagataatg
     7621 atcgcgttta tcgacctgga taacttcaaa ttaatcaacg atcgttttgg tcataatagt
     7681 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
     7741 gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg
     7801 gatatttcgt cgctgcgaga acgcattcag cagcaaatac gcggagaata tcacttaggt
     7861 gatgttgatt tgtattatcc tggtgccagt cttggcatag tagaagtcga tcctgaaacg
     7921 accgatgcag acagtgccct acatgctgcc gatatcgcga tgtatcagga gaaaaaacac
     7981 aaacagaaaa caccttttgt cgcgcattca gcgccacatt cctgaggcgt attcacatcc
     8041 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc ttcattacag
     8101 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
     8161 gaaaaaaaca acgatcatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct
     8221 gggatggtgg taacgtcaca tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
     8281 caatttacgc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
     8341 acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagcgtg
     8401 ctgatccaac cgccgactac ggcaccgact atccccagca ggatagtcat aaagaatcca
     8461 ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata
     8521 atccatgaca gaatgcccat tgtttcctca cttatctgtt ttgcattagc gggttagtcg
     8581 ctgataaaaa gcatagcaca acatcgggag ggcaagattt gtgacgagca tcacggagga
     8641 tttttttgcg atggcgcaga aattgcgcca tcaacgatca gtgataatta ccaaccacaa
     8701 acatcatgtt cgttttccgt gtcataagaa cgcacggtat tcaccagatc ttttatcact
     8761 tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata atcgacagaa
     8821 acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat gattcatttc
     8881 ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg agccacgaag
     8941 aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca cttttctgga
     9001 gggaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc tgcactgtgt
     9061 tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg tgggtaaacc
     9121 cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc agtttgttta
     9181 ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag catccttttc
     9241 cctccttcgg tgaatgcgct gaaaacggtt tattccagcc gtttcagggt acgcctgata
     9301 atttgcattt taaataccat ttattggtta ttttttagca ccatatcaga gaagaatcag
     9361 ggaggattat agatgggaag cccgcgacaa cgcgggctga aagcatcagg attgcagcgt
     9421 cgccagtcgg gcagcgaaac ccacgaacat caaaccaatc agtgagttgc ccactttagc
     9481 cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagatataa tcaggaagct
     9541 caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg aaattcccgt
     9601 atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca aaatggcttt
     9661 cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gcaccgtatt ggggctcatc
     9721 ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac tccccagata
     9781 gagcaaataa aatgcaccaa gataacgtac aatgttgaat aatatcgggg tggtcttaat
     9841 taatgtcgcc actccagccc atgccagaaa catcaatacc gcatcgccaa taaatacacc
     9901 gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga gtacaaacag
     9961 ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg tccagtaatt
    10021 cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat catgcgcgat
    10081 taaaggtgaa tatgctaacc aatctgtagc agcttagaaa ggagaaaatc aggttttaac
    10141 ctgatatcaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa aattcgggta
    10201 gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg ctgacgtcgc
    10261 cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg gtagcacttg
    10321 cactaaatga ccactggcaa tgttttcgct aacatcccac caggaacgca gagcaatccc
    10381 ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca gtgggccggt
    10441 gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa aaggatgatc
    10501 gcgctcttta atgaccaggc acggcaatgc agaaagatcg gttaaatgtt tcggcgcgcc
    10561 atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattggtcg ccagtttgcg
    10621 ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata tcgagatcga caccttcatt
    10681 gaccaaatcg accagccggt cttccacatc aaaacgtaat tccagttgcg gatacgcctt
    10741 cgccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc tgctgataat
    10801 acgcagcatc ccctgtggca cctgacgcac gtcagaaagt tcgtccatca tctgaccgac
    10861 atcctgcaaa atcagctgcg cccattcata aatccgctct ccttcttcgg taatggtgac
    10921 gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca aggcgatgcg
    10981 cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggtggcaa aaccagcccg
    11041 gcgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat tcatgattcg
    11101 tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat aggatggtga
    11161 tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat gcgtattgct
    11221 gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg cgtgttacag
    11281 gctgccgctg agcgctgggg cttcgccttg agttttgagc aaatggagtg ggcgagctgc
    11341 gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca acttagccgt
    11401 ttcgatgcca tctattttgg tgccgtcggc tggccggaca ccgttccgga ccatatttcg
    11461 ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa cctgcgcccg
    11521 gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg cgacatcgat
    11581 ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg tagagtgaat
    11641 gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg tggtgtcgat
    11701 cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac gctaacttct
    11761 gctactaaat caaacggttt agccatcagc atgccgtact gggatgagcg agtggaagca
    11821 atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat tctctgcgcg
    11881 cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt gtttggcgat
    11941 atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc atccgccaac
    12001 ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg tccacggttc cgcgccggat
    12061 atctacggga aaaatattgc taaccctatc gccacaattt gggccggtgc aatgatgctc
    12121 gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat tctggcagcg
    12181 attgaagaag tgattgctca cgggccgaaa acaccggata tgaaaggcag tgccaccacg
    12241 ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac cagtttattt
    12301 gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agttaataac ctgcgtcatt
    12361 tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa ttgtttaatt
    12421 tatgtaacat gcaaataatc aatttgttaa acacaacata caactcatta ccctgcaaat
    12481 aattacaact ggcaaaagcc agagacgggc ttttatgccc tggagatgac tcatgatgag
    12541 caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca ttcttttcat
    12601 cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg caatatacac
    12661 ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg caatgggact
    12721 ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag gaaaaccgga
    12781 atacagcacg ctctcctggc tgtttatgtt tatttgtgtc ggtttaggtt cttctacgct
    12841 ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa atatcgcacc
    12901 gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact ggggcatcag
    12961 cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc atgtgcggaa
    13021 aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc gcccgcaagg
    13081 cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg cactgactat
    13141 ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcactga ccggtttacc
    13201 cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt tttgcctaag
    13261 ctcatggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg gctggggcgc
    13321 gctcctgctg ccattactgg tgctgattgt cggtccaacc gaatttatta ccaacagcat
    13381 catcaatgcc atcggcctga ccacgcaaaa cttcctgcaa atgagcttat tcaccgatcc
    13441 gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt ggtggatctc
    13501 atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta agattaaaga
    13561 agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct tcggcgtaat
    13621 ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac aggtgctgga
    13681 aactctgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag ctggtaaatt
    13741 gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata tggatgcagt
    13801 ggcctacacc atggcggcga ccagtacgcg taatctccag gaaggtgacg atcctgaccg
    13861 tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca tcttgtttac
    13921 cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc ccttcctcgt
    13981 cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt acgccgacat
    14041 tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc tggaaaaaac
    14101 accagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat catcctaacg
    14161 ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa aatttttgcg
    14221 ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag aacgcgtttg
    14281 aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac agcagcgaac
    14341 tggcgaatgc caatgattat gtgacgcggg agatcattgg cgaaagcatc gtgctggtac
    14401 gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt ggtcatcagt
    14461 tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg cccatatcac gcatgggcat
    14521 tcaaactcga tggcaacctg gcccatgcac gtaactgcga aaacgtcgcc aatttcgata
    14581 gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc gtcttcatca
    14641 acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc gcgaaagtgc
    14701 tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc acccgcacgc
    14761 ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt ggtccggcgc
    14821 atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg cacggtaact
    14881 ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt gaagagggta
    14941 cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac gtcaccccga
    15001 tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact accctgcaaa
    15061 actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg ctgattgagt
    15121 ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta cagaaagggc
    15181 tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc ggtagcggca
    15241 tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt aaggactaat
    15301 gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac agcaatgtca
    15361 gactatcaaa tgtttgaagt acaggtgagc caggttgaac cccttaccga acaggtgaaa
    15421 cgcttcacgc tggtggcaac cgatggcaaa ccattacctg cgtttactgg aggaagtcac
    15481 gtcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc actgctgagt
    15541 tcgcctctcg atacaactca ttatcagatt gccgttcgac tggaagaaaa ctcgcgcggc
    15601 ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat ctcaacgcct
    15661 aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc gggcggtatt
    15721 ggtatcaccc ctttcctgtc gcacatggca gagttgcaac acagcgacgt cgactggcag
    15781 ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct agtccagcat
    15841 ccgcaggctg agaaagtcca tttgcatcat tcatcaaccg gaacacgact ggaattagcg
    15901 cgattattgg cggatatcga acctggcaca cacgtttata cctgtggccc cgaggcgcta
    15961 attgaagcgg taagaagtga agctgcgcgt ctggacatcg ccgccgatac gctgcacttt
    16021 gagcaatttg ctatcgaaga caaaatcggc gatgcattta ccctggtgct tgcccgttcc
    16081 ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga aaataataaa
    16141 gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga aacggcaata
    16201 ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg tgccagccag
    16261 caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt ggatttgtag
    16321 tttgccgaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag attttactgc
    16381 ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg ccagccggaa
    16441 atcagctccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag cagttggttg
    16501 atttgccgac gcgatgccag caattcggcg ctgatcttgt gcgtttcgct cacgtcagta
    16561 atcagcgact taatcgcttt aaacgcttta cgataacccg gcatgtccat caggttaagc
    16621 atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag cgccagcagc
    16681 gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag ttcgcctaaa
    16741 cttcccggca tataacgcgc taccgaccac aaatgctctt cacgcacgac aaagttcacc
    16801 gccagatcgc gctcacgcgc cttgcgcagt cgccagtcgg ctaacagttg cagacaggcc
    16861 agttggcgtg tgcgtaattg ccaggcattg gtgatatcac gccaggcatc ttccggcgca
    16921 acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc aggtagccag
    16981 ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata ccagacatcc
    17041 gccgctgcat attcacactg acgttcggtc agcggtctgg ccagccagtc ggtgcgcgat
    17101 tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa accccatgac
    17161 atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaggggttg tggtaattcg
    17221 ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa ttttgtgatg
    17281 gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag aggatcaatt
    17341 agcgccagat gctcaccatc aaaaagttga atcaacccca gctgcgggta ataagtgcgc
    17401 gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc ttcacacaaa
    17461 gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgtattc tctttagtgg
    17521 gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacgc tcaggcttta
    17581 ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt tccgacgtta
    17641 gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc cgtgagctga
    17701 cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt cactacaaag
    17761 attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac ttcctgtacg
    17821 ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa accggaaacc
    17881 agaatcatgt cttttttacg atcgacaatg cgcaggaacc cttcttcatc catcaccgcg
    17941 atgtcgccgg tgtgtaacca gccatttttg atgatctcat cggtagcatc cggacgctgc
    18001 cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg acccggtggt
    18061 acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg caaaccgatg
    18121 ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc acactcggta
    18181 aggccatagc cttccagcag atactgtcct gtcagtttca cccaacgctc tgccaccact
    18241 tgctgcactg gcatcccacc gcctgcggaa agatgcagac tggagaaatc cagctgctgg
    18301 aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgccagtgat agcggtaaac
    18361 ggatatttcg ctaactcttt taccaaccct ggaatatcac gcgggttagt gataagcagg
    18421 ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa aatgtgatac
    18481 agcggcagtg ccgttactac cagttcttta cctggatgca gcaacggccc ataggtggcg
    18541 ttgacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc tttcgccaca
    18601 cctgtggtgc cgccggtgta ttgcaggaag gctaaatctt ccggcaccag ttcgggtttg
    18661 acgtactgca tccggtagcc gttatgcagg gcgctacgaa atgaaatggc atctggcaga
    18721 tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac cgtgcctttt
    18781 gccgtagata gctgatcccc catacgggtc agaattacgt gctgaacggc ggttttatca
    18841 accacttttt ccagtgtgtg agcaaagtta gacacaataa caatcgccga tgcgccgcta
    18901 tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt tacgacgatc
    18961 atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt aggcatcatc
    19021 aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc ggcaaacgcg
    19081 cgactgcgtt cttccagctt gcggaaggtc atcacctccc ccatattcac aaacgcaggc
    19141 tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg ataacggtca
    19201 gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt cttcaattct
    19261 tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca tgccgttaac
    19321 ataatattaa ctcatcatac cagcttgata attacccaac gaaaaggttg cgaagcgcgt
    19381 cactatttat ttttatcttt accgtaagaa tgcagaaaca gcggaccagc cgctgtttct
    19441 ttttctttaa aaacaagcaa ttattcagtt acaactgttt gtactctcgc ggggccggga
    19501 ttataccagc cccatccgcc gtagccatag ggccagccac gaccgccata aaaccatgga
    19561 tcaatcggct gaggcggcat aatcacctgc tgggttaaat gccaacgttt gtaacccgtt
    19621 acttgcatca ccataaattt atagggcgta ttgccgattt tgccgtcaac cgcaccagtg
    19681 attggcccga ctaccgtaac cagttgtcca cggaaatcca ccgggtccag aaaaccgttc
    19741 acatcggcat aaatgcgacc gcgagaaggt tctcccagcg tcggtctggc tccgctgtcc
    19801 agcggtacgg tagcaatttc caggcgggtt ttcccttgct ggttttgtac cgcaaccact
    19861 ttgccaccaa agcgtgcctc ctgaccaacg tacagctgcg gcgcactcat cacccgaact
    19921 aaatcttgtt gcggcgtggg actactgcct ttaatggcgt ccggcacagt gacacaaccg
    19981 ctcagcatta gcgcaaacgt acctgccagt atgcctttga taacattctt ttgaaccgcc
    20041 atgatgcgac tccttttttt ctcagggcat actcttaaga ttcattcttt gcccggaagt
    20101 ttcttccatg cgacgttgtt acgtaaataa accggttcgg catgttccac cgccaccgtt
    20161 ttaccctcgg caaacatctg acaagcaatc ggcagcatat cttctgcagc gggcagtaaa
    20221 acttctccgt cgcgcaaaac cagtccgctc tctttaccga gatccggcca ggcttgccag
    20281 cctgtaccta ccgtcaccca ttccccggaa agctgttgca tccgttcatg aacgagttcg
    20341 ggtttgagta cggcttcggt ttcttcgccg tgccagacgc cgttttcatc acgctgatat
    20401 tcggcccagt aaacttcgcc catccgcgca tcaatggcag ccagcacgcg cgttgcgccg
    20461 tttttgcgcc acgccccctg cgccatcgtc atgagcgtgg agacgccaat catcggcagc
    20521 gccgcgccaa gtgccagccc ctgcgcaatg cctataccaa tgcgcacgcc ggtaaaacca
    20581 ccggggccgc gcccgtaggc cagagcatca atatcagaga gggaggtacc gctggtggtc
    20641 aggatatcct gtaccatcgg taagattcgt tgagtatgtt cacgagggca aagctcaaaa
    20701 tgagcgttga cagtaccgtc gttccacagg gcgacagagc aggcctctgt cgcggtatcg
    20761 atagccagaa ttcgcatggg tcttcgtgct tagatcaata aaaaggcgcg catcatacca
    20821 tactccgtaa caaattacct ggaggatggt atcgcaagga aacgaaccgc acgggcaatg
    20881 tcacgggtgc gcggcgcggg cggcagactg gcgagaaacg tcgcaccgta agggcgcatc
    20941 acaagccgat tatcgcaaat caccagcacg ccgcgatcat cggcgtcgcg aatcagtcgc
    21001 cctaccccct gcttgagagt aatcaccgca tccggcagtt gtacttcatc gaacgggtcg
    21061 ccaccgcgca aacggcaatc ttccatgcgc gcttttaaca gtggatcatc aggagaggta
    21121 aacggcaatt tgtcgataat caccaatgac agcgtatcgc cacgcacgtc caccccttcc
    21181 cagaaactgc tggttgccac aagaagcgcg ttaccggcgc tgacaaactg ctgcaacagt
    21241 tgccctttgc tggtttcccc ctgcaacaaa acgggaagcg tcatggtagc gcggaactgc
    21301 tcggccagat cgcgcatcat ggcgtgcgag gtacaaagca taaaacaacg accgttgtta
    21361 gcttcgatga tcggtcgcag catagccgcc agtttccgcg cggaacccgg ttgattggtt
    21421 tgcggcaggt tgcgcggcac acagagtaac gcctggcggc tgtaatcaaa cgggcttggc
    21481 aatagcaacg actccgcctg ttctatccca agccgcgagg tgaaatgatg cagatcgtcg
    21541 ttcaccgaca gcgttgctga ggtgaagatc cagctaccgg gcttttgcgc cattaactct
    21601 ttgaatttat ccgccacgct gagcggcgtg agagccagag taaaatggcg cgaagtgcat
    21661 tcgtaccagt agctgtagcc cggctgattg atctctttta gccgcttcag ccgtgtgcga
    21721 tacaacgtgg cgcgctcaaa tgccgcatcc agcaaggcag aacgccccag tgacagtttc
    21781 gccacgtcat aacaaagttc cagggtgtca tcgagcagta aaaatgcccg ctgaatttgc
    21841 ggattagcta acagctcacg caggttaccg cgataacctg gttcaccgag ttgcagacga
    21901 aaatcctgcg cactctgagc aagacgatct gcgcactttt gtaactgctg ggtgtctttt
    21961 aattcggtgc ggtaggcgat ggtgatgtct tttgccaggt cgagcagttg tcgactggag
    22021 agtgactgac caaaatactg gctggcaatg tccggtagct ggtgggcttc gtcgaagatc
    22081 atgacgtccg cttccgggat caactcgcca aatccactct ctttaaccac catatccgcc
    22141 agaaagagat gatggtttac caccaccaca tcggcgtcca tcgctttttt acgtgctttg
    22201 accacaaagc aatctttata catcgggcag tcgctgccaa gacagttgtc gttggtgctg
    22261 gtgaccagcg gccacgcctg cgaatcttcc gccacgctga cgcaggtgct gatatcacca
    22321 tcgactgttt gattagacca ggagcgcagc aggatcacat cgcttaatat ttgtaccgga
    22381 agatcgcccc ccgccagcgc ctgctgttcg agacgttcga ggcagaggta gtttgagcgc
    22441 cctttcagca acgccacgtt gcccgtgtat ttcaacgcct ttgagaccgt cggcaaatcg
    22501 cggctataga gctgatcctg caacgctttt gagccggtcg agataatgac tttctttttc
    22561 gcccgcagcg caggggccag gtaagcgtag gttttgcccg taccggttcc tgcttccacc
    22621 accagcggct ggcctttttc tatcgcctgg gtgacggcta ccgccatctg tcgctgtggt
    22681 tctcgcggct taaagcctgg tatcgctttc gccagctgac cgtctggtgc aaaatcgtcc
    22741 gtcacactac cccctgttga tttgaacagg gattatgtca ggatgagggc gctttcgcca
    22801 gttgaagtgg tgacggcgac ctcacattgt ggcagtcttt gcagcacgaa aatggaagga
    22861 taatgaggac aaaatgatga ctatcgttcg tatcgatgct gaagcccgct ggtctgatgt
    22921 agtaatccac aacaacacgc tctactacac tggtgtaccg gaaaacctcg acgccgatgc
    22981 ctttgagcaa accgccaaca cgctggcgca gattgacgcc gtgctggaaa aacagggcag
    23041 caataaatcg agtattctgg atgccaccat tttcctggcc gataaaaacg acttcgcggc
    23101 gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat gcgccggtgc gctgcacggt
    23161 acaagcgggt ttgatgaacc cgaagtataa agttgaaatt aagattgtgg ctgcggtgta
    23221 agctttatcg aagcaaaata agtcagacga taatttatcg ataatactgg tcggttttac
    23281 ataaatcgac caga
//
LOCUS       DAZGCQ010000079        22831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000079 DAZGCQ010000000
VERSION     DAZGCQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.079"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            303..1073
                     /gene="yjjP"
                     /locus_tag="ACHK07_003417"
     CDS             303..1073
                     /gene="yjjP"
                     /locus_tag="ACHK07_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO8103704.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1064..1537
                     /gene="yjjB"
                     /locus_tag="ACHK07_003418"
     CDS             1064..1537
                     /gene="yjjB"
                     /locus_tag="ACHK07_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO8103705.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1644..2183
                     /gene="dnaT"
                     /locus_tag="ACHK07_003419"
     CDS             1644..2183
                     /gene="dnaT"
                     /locus_tag="ACHK07_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO8103706.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKTEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2186..2923
                     /gene="dnaC"
                     /locus_tag="ACHK07_003420"
     CDS             2186..2923
                     /gene="dnaC"
                     /locus_tag="ACHK07_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO8103707.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2972..3466
                     /gene="yjjA"
                     /locus_tag="ACHK07_003421"
     CDS             2972..3466
                     /gene="yjjA"
                     /locus_tag="ACHK07_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO8103708.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3720..6011
                     /gene="opgB"
                     /locus_tag="ACHK07_003422"
     CDS             3720..6011
                     /gene="opgB"
                     /locus_tag="ACHK07_003422"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO8103709.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQTVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6149..7171)
                     /gene="lgoD"
                     /locus_tag="ACHK07_003423"
     CDS             complement(6149..7171)
                     /gene="lgoD"
                     /locus_tag="ACHK07_003423"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO8103710.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATLEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7310..8224
                     /gene="lgoR"
                     /locus_tag="ACHK07_003424"
     CDS             7310..8224
                     /gene="lgoR"
                     /locus_tag="ACHK07_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO8103711.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVMARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8439..9800
                     /gene="lgoT"
                     /locus_tag="ACHK07_003425"
     CDS             8439..9800
                     /gene="lgoT"
                     /locus_tag="ACHK07_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8103712.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9849..11513)
                     /gene="tsr"
                     /locus_tag="ACHK07_003426"
     CDS             complement(9849..11513)
                     /gene="tsr"
                     /locus_tag="ACHK07_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO8103713.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11741..12283
                     /locus_tag="ACHK07_003427"
                     /pseudo
     CDS             11741..12283
                     /locus_tag="ACHK07_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12576..14726
                     /gene="btsT"
                     /locus_tag="ACHK07_003428"
     CDS             12576..14726
                     /gene="btsT"
                     /locus_tag="ACHK07_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO8103714.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14776..14979
                     /gene="yjiX"
                     /locus_tag="ACHK07_003429"
     CDS             14776..14979
                     /gene="yjiX"
                     /locus_tag="ACHK07_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8103715.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14990..15946
                     /gene="yjiA"
                     /locus_tag="ACHK07_003430"
     CDS             14990..15946
                     /gene="yjiA"
                     /locus_tag="ACHK07_003430"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8103716.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILMTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15992..16906)
                     /gene="mrr"
                     /locus_tag="ACHK07_003431"
     CDS             complement(15992..16906)
                     /gene="mrr"
                     /locus_tag="ACHK07_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HFO8103717.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            17094..20606
                     /gene="hsdR"
                     /locus_tag="ACHK07_003432"
     CDS             17094..20606
                     /gene="hsdR"
                     /locus_tag="ACHK07_003432"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HFO8103718.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQSQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDSLLDKFSDYIWDELA"
     gene            20683..22272
                     /gene="hsdM"
                     /locus_tag="ACHK07_003433"
     CDS             20683..22272
                     /gene="hsdM"
                     /locus_tag="ACHK07_003433"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HFO8103719.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPVGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYSGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PSTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            22269..>22831
                     /locus_tag="ACHK07_003434"
     CDS             22269..>22831
                     /locus_tag="ACHK07_003434"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8103720.1"
                     /translation="MSLKNIPTNWCVTTIGDINDYKSLTFNPKSYQEDSFELYSVPSF
                     ASGSPEILLGKDIASNKQYIQFKDILLCKINPRINRVWCVKEKKEHPQIGSSEWIVIR
                     QPLIHEGFLAYFLQTQDFRDLLCSDVSGVGGSLTRAQPKRVATYNVPIAPLPEQKIIA
                     EKLDTLLAQVESTKARLEQIPQILKRFR"
BASE COUNT         5663 a         6008 c         5692 g         5468 t
ORIGIN      
        1 aatcaatatt tattcaataa aatctgatag ttttaacttg ttaacattaa aaaacgcgcc
       61 cagtcaccta ttatcctgat tcgtaaaggc taaataatga attcattata gtgataatat
      121 tcatgtgata atttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttctcccg
      181 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      241 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      301 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      361 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      421 cgctgggaat ggacagcgta gaaagttcta tctcttcgaa cgccatagtg ctgacaacta
      481 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      541 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
      601 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      661 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      721 gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag ctgctggcac
      781 aacgtcatct tcatccacaa atcaactttt gcctcaccgc tttcgccgcc accaccattt
      841 ccggattgct tttgcaactc cccactttca gcaatacccc caccattgcg atggccgcca
      901 gcgttctgct gctggttccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      961 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
     1021 gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1081 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1141 ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct cgataggtca
     1201 tggttcccga atgatcttga tgaccagcgg gttgaatatt gagtggtcaa cctttatggc
     1261 ttcgatgctg gtcggtacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1321 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
     1381 gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
     1441 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1501 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
     1561 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     1621 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     1681 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa aactgaaggc ggtgtggttg
     1741 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     1801 tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgatctg gacgatcaac
     1861 tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc
     1921 cggactggca acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcgctaa
     1981 gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
     2041 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     2101 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa
     2161 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     2221 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
     2281 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     2341 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     2401 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
     2461 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
     2521 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     2581 catcaccgtg gccgatatta tgtcagcgat gaaagatacc ttcaggaata gcggtaccag
     2641 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
     2701 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
     2761 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     2821 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
     2881 ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt ttctggcggt
     2941 atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg
     3001 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     3061 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
     3121 ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga aaccaggcct taagcgcaga
     3181 taacatgaac aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcgtcggt
     3241 aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
     3301 gcaaaaagaa gacaccaact atctggacgg cattcagggt ttgctgaaaa ccaaagatgg
     3361 tcagcaactc aatctggata acatcggaac aactccgctg gcagaaaagg tgaaaaccaa
     3421 agcctgcgat ctggtgttaa aacaggggct gaacttcatt tcctgaaccg cgaaacgccg
     3481 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     3541 gttttacccc tgttacacgg gaagtgaccg taatcaaaaa agtctgttta taaactaatt
     3601 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     3661 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
     3721 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     3781 cgggacgtaa cacctggtgg tttgcagcca cgttaacggt gctggggcta tttgtcgttt
     3841 taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc
     3901 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta
     3961 tcggcattgt gctggggctg acggcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     4021 gtcgtcatca tccgcaccat tttggttaca gcctgctggc actcttactg gcgctgggtt
     4081 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     4141 atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
     4201 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgaag
     4261 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     4321 acacgcagca gctgccagga acggattaca cgattgcggg catggtggct tctcagtgcg
     4381 gcataccgct gtttgccccc tttgaaggca acgcctccgc ttctgtttcc agcttcttcc
     4441 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
     4501 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc
     4561 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
     4621 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
     4681 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     4741 ttatctcgcg cacctgtaac cgcaaaaaat atgattttga cggtaagccg aatcagtcat
     4801 tcagcgcggt aagttgcagc caggagaata tcgcgacgtt tatcaacaaa atcaaagcgt
     4861 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
     4921 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
     4981 acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg
     5041 tactggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
     5101 agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccagata
     5161 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     5221 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     5281 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg
     5341 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
     5401 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcag cttggcggtc
     5461 agcaaactgt tcagcatgtc gacaaaacaa catggaagag caagacggca tttaaagata
     5521 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
     5581 atattcgcta caaagccgac agtttcatct ttaacgtcgc cggtgcgcca gaagaggtga
     5641 aacagtttag cgggatttcc cgtccggagt cgtggggccg ctggtccaac gcgcagctgg
     5701 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     5761 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     5821 aacaaactct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     5881 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     5941 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac
     6001 gtgaagggta attcctgact gatgtgtagg tcgcataagg tgcgacctac acaccttctc
     6061 cggcttacaa cacattatta agaaacaaaa aaaacagcgc ccaacacttc cccggacgct
     6121 gcatggcaac tttaataatc tcgccttatc agaaagtaat tacgccttta attaactcac
     6181 gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg
     6241 ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact ttagcaaaat
     6301 cttccagcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg aattcaggat
     6361 cggagaactg caactctcct ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa
     6421 tcagattcac ggtgttattc atcgcatgtt gattgcctgt cgcgtcgatc actttctgcg
     6481 ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa atcttcggct gacgggtcca
     6541 gcacgggcaa ctccagacgc gttgccacat gttcacggcg cgccggactg gtatccgcca
     6601 ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg
     6661 ccccaaccac cagcacctgc tcgccgggag caattgctgc gcgacgcacc gcatgagcgc
     6721 taatagcgaa aggttcaatc aatgcggccg cctgcgggtc aataccgtct gcgggcaaaa
     6781 tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga
     6841 ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg
     6901 caacataagg gatcacagca acttgctgac catttttaag atcagcaata tttttaccca
     6961 gcccaacaat ctccccacat atttcatggc ctaaaacccg tggataacta aaaaatggtt
     7021 gatttccacc ccaggcatga atatcggtac cgcaaatccc gacagactta atttttatta
     7081 atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct
     7141 gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt tgttgcctta
     7201 gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg aaaacacacg
     7261 cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc
     7321 acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat
     7381 cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcactac
     7441 agtgcgtcat attctcagcc acttacgcga atgcggcgtc ttaacgcagg ttggaaacga
     7501 ctatgttatg gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta ccgcttcaat
     7561 gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgtcagtt
     7621 acgccctggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt
     7681 ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaagcg
     7741 cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt ttgagctacg
     7801 cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg
     7861 ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca acatcggcaa
     7921 tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc
     7981 cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta
     8041 tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg agcatatgac
     8101 cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca
     8161 tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca
     8221 ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc aaaagataaa
     8281 taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg tttttgtgag
     8341 cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt aatgcacaat
     8401 tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa aatatcaccc
     8461 tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg ccaccagatg
     8521 gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg ttgttattat
     8581 tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca aatttaacga
     8641 ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc gtgttttcac
     8701 tcgcttacgg cattgcgcaa cttccttgcg gcccactatt ggatcgtaaa ggcccacgcc
     8761 tgatgctggg actggggatg ttcttctggt cactgttcca ggccatgtct ggcatggtgc
     8821 acagctttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa gcgccgatga
     8881 acccatgcgg tgtaaaagtc attaacgact ggttcaacat caaagagcgc ggacgcccga
     8941 tgggcttctt caacgcagct tctaccattg gcgttgccgt aagcccaccg attctggcgg
     9001 cgatgatgct ggtgatgggc tggcgcggga tgtttattac cattggtgta ctggggattt
     9061 ttctcgccat cggctggtat atgctctatc gcaaccgcga gcacgtagaa ctgactgccg
     9121 ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg ctcagttttg
     9181 ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg aatgatgctc ggattcagtg
     9241 gcatcaacta cactgcgtgg ctgtatctgg cttggcttcc tggttacctg caaacagcct
     9301 ataacctgga tttaaaaagt acagggttga tggcggctat ccctttcctg tttggggctg
     9361 ccgggatgct ggtcaacggt tacgttactg actggctggt caaaggggga atggctccga
     9421 ttaaaagccg taagatctgc attattgccg ggatgttctg ttctgccgcc tttacgctga
     9481 tagtaccgca agcgacaaca tccatgacag cggttctgct gattggtatg gcactgttct
     9541 gtattcactt tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca
     9601 tgactgcgtc ggtgggcagt atccagaact ttgccagctt catctgcgcc tctttcgcgc
     9661 cgatcattac tggttttatt gttgatacaa cccactcatt ccgtctggca ctaatcattt
     9721 gcggttgcgt caccgcagcg ggagcactgg cgtacatctt cctggttcgt cagccgatca
     9781 acgacccgcg taaagattaa actccctatg cccgataaca ttttgcttat cgggcatttt
     9841 tatggcgatt aaaacgtttc ccagttctcc ccgctatctg ccactgccat tttacgcggc
     9901 gtagctggcg tcacggtttt taccacagcc gatgtttcac gctgctgttg ctgctgttgc
     9961 tgaatccgga acactgccac agcttcggtc aggcgactgg cctgctcttc cagcgcggcg
    10021 gcggcagcgg cagattcttc caccagcgcg gcgttctgtt gagttacccg gtccatctca
    10081 gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc agaagctatt
    10141 tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa tctccgccat tgtttccccg
    10201 gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt
    10261 ttaatttcac gagccgcctg ggcgctacgc tgggccagat tacgcacttc tcccgcaacc
    10321 accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc gttcaaagcc
    10381 agaatattgg tctggaaagc aatgccgtca attacgctga taatatcggc gattttctgc
    10441 gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt gccgccgcgt
    10501 tgcgccgttt cagaagcact taacgccagg tggctggcct ggcgcgcatt ctcggcgttc
    10561 tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc
    10621 tgttgctcgg tgcgcgaaga gagatcgtta ttaccggtgg cgatttcgct ggcaccgcta
    10681 tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata
    10741 tggcgcaaac tttctgccag ttgccccatc tcattagagc catccacctc aatcggtttc
    10801 accagatcgc cgcctgcaat atgacgaatg ctgtcaatca gacgattcat tggcgctacc
    10861 agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac gatcatcacg
    10921 cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac ggcgatatca
    10981 tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat
    11041 ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag ttggatcagc
    11101 tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg
    11161 ctctgacgcg ggtcacgcgg caacgcttcg taatctgccc agtttttttc cgcctgtttc
    11221 agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc
    11281 tgatccatca tgtagcggat acccgcacgg ttgagggtgt tacgcgtctg caacaacgcg
    11341 acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt
    11401 tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg taaaaggcca
    11461 aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct
    11521 ctttcctgtg gaccgatgca agattttcgg cctgaaatga gaaaacttta taggtagcga
    11581 cttgaaactg gttaatttta cctcactttt ttaatttttg ttcaaaaata caataaccac
    11641 ttattgatac attttctttt cttatttttt tcacacgaat tgtatctatt ttgaacgaaa
    11701 tattttatag agtgtggaat tttaagaaag gacatctgaa atgttaaacc gtgacttcga
    11761 tcatctctca acgctattac atctcttttt tgaacgtgag gatatttgcc aaaagcttaa
    11821 tattattgac caaaacaata tcaccggttg ggaagtgtgg ttccaggtag agtttgctaa
    11881 tatgctctgc tcaacagacc atgaatggtg gcgtgaacaa gcactttcct gtgatatgcg
    11941 caaaaaacct gagcgcccga cactacgcac agatttctta ctacgtaaaa aaggctgggc
    12001 acaagatagc tatatcacgc ttgaaataaa acaaaacaga gacgcgacca gttgtgttaa
    12061 aaatatgatc gcagacttag aaaaaagcgc aaaaataaaa cgttccgaac tcgatttacg
    12121 cagtttctga accttgggta tcacccctat gatagacagg gaaaggctgg actctctcat
    12181 cgattgctat ttagatgaga aattttatct tactaaatct cgtaaaaaac acgttttact
    12241 taaagaaata aataacacac catattgcta tattgttatt taatatttat agcatgaata
    12301 taatttttaa atgagttaca caaccgaggg ctaaaaatcg cttcggttgt gatcatcatc
    12361 acataaataa ccttcataaa ccttgctcaa aaccgctcaa ccgcaaaact gaccgcttac
    12421 gtccctaaaa taaccactca gttatttacc ttactttacg cgcgcgtaac tctggcagca
    12481 tcactgcagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc
    12541 ggccaactat taatcaatac atgccaggtt ttactatgga tacgaaaaaa ctattcaagc
    12601 acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta gttgcattac
    12661 gtcgggggga gcacgtcagt gccctgtgga tcgtggtcgc ctctgtgtcg gtgtatctgg
    12721 tggcgtatcg ctactacagt ttgtatatcg cccagaaggt gatgaaactc gaccccacgc
    12781 gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc aaccgttacg
    12841 tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtt ggtccggttc
    12901 tcgccgcgca gatgggctac ctgcctggca cactgtggct gctggcgggg gtagtgctgg
    12961 ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac ggcgcatctc
    13021 tcggtgagat gatcaaagaa gagatgggac cagtaccggg gactatcgcg ctgtttggct
    13081 gtttcttaat catgatcatc atccttgccg tcctggcgct gattgtggtt aaagccctgg
    13141 ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accaattgcg ctgtttatgg
    13201 gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtc attggtatcg
    13261 tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg tactggggtc
    13321 cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat gcgtttgttt
    13381 ccgcgctgct gccggtgtgg ctgatcctcg caccacgcga ctatctggca accttcctga
    13441 aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg gaactgaaaa
    13501 tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc gctctgttcc
    13561 cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgca ctgatctctt
    13621 ccggtactac gccgaaactg ctggctaatg aaaccgacgc gcgtttcatc ggttatggcg
    13681 caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc atcatcgaac
    13741 cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc atgcctaacc
    13801 tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagttaaaa gacgttaccg
    13861 cacacgcggc ggcgaccgtc agctcctggg gcttcgtgat ttcgccagag cagatcctgc
    13921 aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt gcgccgacgc
    13981 tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac atgggcttct
    14041 ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg gatgcgggta
    14101 cccgttctgg ccgctttatg ctgcaagatc tgctgggtaa cttcatcccg ttcctgaaaa
    14161 aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt ctgtggggct
    14221 acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg tggccgctgt
    14281 tcggtatttc caaccagatg ctggcagccg tagcgctggt actgggcacc gttgtgctga
    14341 ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta tggctgctta
    14401 tctgcaccac ctgggcgctg ggtctgaaac tgttcagtac caacccgcag atggaaggct
    14461 tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac ctgacggcgc
    14521 agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca ggcctgagta
    14581 ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc tggcttgcgg
    14641 tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc ccggaaggcg
    14701 gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg ggctttgttc
    14761 ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct cggccaggcg
    14821 gcaaagatgt tgattggcat tccagactac gacaactacg tcgagcatat gaagaccaac
    14881 catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc
    14941 tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca tgaacccgat
    15001 tgcagttacc ctactcaccg gttttttagg cgcgggaaaa accaccctgc tgcgccatat
    15061 tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg gcgaagtctc
    15121 cgttgatgat caactgattg gcgaccgcgc cacgcagatc aaaacgctga ccaacggctg
    15181 catctgctgt tcgcgctcca acgagctgga agacgcacta ctcgacctgc tggataatct
    15241 cgacaagggc aatattcagt tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc
    15301 cggcccgatt attcagacct ttttctccca tgagatttta tgccagcgtt acctgctgga
    15361 cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc agttcaccat
    15421 cgcccagtcc caggtgggct acgccgaccg tattctgatg accaaaaccg acgtcgcagg
    15481 cgaagcagaa aaactgcgtg aacgcctggc gcgcatcaac gcccgtgcac cggtctacac
    15541 cgttacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt ttatgctgga
    15601 agaaaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac aaaacgatat
    15661 ttcgtcgatt gtggtagaac tggattaccc ggtagatatc agcgaagttt cccgggtgat
    15721 ggaaaacctg ctgctggaat cggcggataa actactgcgt tacaaaggga tgctatggat
    15781 tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca gcgccgactg
    15841 ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg gtgtttatcg ggattcagct
    15901 gccggaggaa gagattcggg cggcgtttgc ggggttgagg aagtaagggg ttatgggccg
    15961 gataaggcgc agccgcatcc ggcctgatat ttcactcaaa atagtccata tccagtttcg
    16021 gcaccttcaa caaacgtgaa gaaaccccta cttcgttttc gatcattaag tgcaccaggc
    16081 gttccccatc aaccaacacc ataccctcga cggattgagc aaagtcacgc gcctgagacg
    16141 taaatccaga agtggtaata aacaccccac gtttcgcctt ttgcccagcc agtgcgccgt
    16201 aaaatgcctg taattctggc ctgcctacag tgttctgcca acgttttgcc tgaacataaa
    16261 ctttctccag gccaagttta tcaagcgata tcacaccatc gatgccacca tctccagtac
    16321 cgccaacacg ctgcaaatca tcacggtggc cgccataccc caggcgatgc aaaacatcca
    16381 gaacaatgac ttcaaagcgc gaaggagaaa cctgcaataa gttttccaga acctcatcag
    16441 ccaccgcatc acgaagctct tttagcgcct gatctaaccg atcgtccggg ctgctctttg
    16501 caagttcttc atgatcggga gagtcggctt tcggatctaa atcgacggca tccggccgtg
    16561 acttaagttt gacattcaca aaatcgaagg ccagatggtt cgtctcctgc tccgtcattg
    16621 gctggggatg agacgcaacc cagtcaaaac ccgcaggagt caggcaccat ttgccacgcg
    16681 acaaactttg cgacaacccg gcacgtttta aacggtcatg cgcccagcct gcacgatttt
    16741 tataaacaag ttgtccgctg gtaatgactt tcgctcgctg gctgtcatcc agtcctaatg
    16801 catccgcggc agcctcatga acatcacgcg cggctgcacc ttccggtttt gtcgccagat
    16861 aacgcagaac aggttcaata aatttgtcat aggtaggaac cgtcatagta catccttgca
    16921 gaatcaggta gatgtttttc ggctactata acactaccaa aatagacgaa cacgttagaa
    16981 atgagtcagt tgctgtgacc gtggtcattg cccggaaagg tacagaaagc taagataagg
    17041 cgttatgggc cttaaatatt tggacaggcc cacacagcaa tggattaata acaatgatga
    17101 ataaatccaa ttttgaattc ctgaagggcg tcaacgactt tacctatgcc atcgcctgtg
    17161 cggcggaaaa taactacccg gatgatccca acacgacgct gattaaaatg cgtatgtttg
    17221 gcgaagccac agcgaaacat cttggtctgt tactcaacat ccccccttgt gagaatcaac
    17281 acgatctcct gcgcgaactc ggcaaaatcg ccttcgttga tgacaacatt ctctctgtat
    17341 tccacaaatt acgccgcatt ggtaaccagg cggtgcacga atatcataac gatctcgacg
    17401 atgcccagat gtgcctgcga ctcggcttcc gcctggctgt ctggtactac cgtctggtca
    17461 ccaaagatta tgacttcccg gtaccggtgt ttgtgttgcc ggaacgtggc gaaaacctct
    17521 atcaccagga agtgctgacg ctaaaacaac agcttgaaca gcaggtgcga gaaaaagcgc
    17581 agagtcaggc agaagtcgaa gcacaacagc agaagctggt tgccctgaac ggctatatcg
    17641 ccattctgga aggcaagcag caggaaaccg aagcgcaaac ccaggctcgc cttgctgcac
    17701 tggaagcaca gctcgccgag aagaacgcgg aactggccaa gcagaccgaa caggaacgta
    17761 aggcttacca caaagaaatt accgatcagg ccatcaagcg tacactcaat cttagcgaag
    17821 aagagagccg cttcctgatt gacgcgcaac tgcgtaaagc aggctggcag gccgatagca
    17881 aaaccctgcg cttctccaaa ggcgcgcgcc cggaacccgg cgtcaataaa gccattgccg
    17941 aatggccgac cgggaaagat gaaacgggta atcagggctt tgcggattat gtgctgtttg
    18001 tcggtctcaa acccatcgcg gtggtagaag cgaaacgtaa aaatatcgac gttcccggca
    18061 agctcaatga gtcgtatcgc tacagtaaat gtttcgataa tggcttcctg cgggaaacct
    18121 tgcttgagca ctactcaccg gatgaagtgc atgaagcggt gccggagtat gaaactagtt
    18181 ggcaggacac cagcggccaa caacggttta aaatcccctt ctgctactcg accaacgggc
    18241 gcgaataccg cgcagcgatg aagactaaaa gtggcatctg gtatcgcgac gtgcgtgata
    18301 cccgcaatat gtcgaaagcc ttacccgagt ggcatcgccc ggaagagttg ctggaaatgc
    18361 tcggcagcga accgcaaaaa cagaatcagt ggtttgccga taatcccggc atgagcgagc
    18421 tgggcctgcg ttattatcag gaagatgccg tccgcgcggt tgaaaaggca atcgtcaagg
    18481 gtcaacaaga gatcctgctg gcgatggcga ccggtaccgg taaaacccgc accgcaattg
    18541 ccatgatgtt ccgcctgatc cagtcccagc gttttaaacg cattctcttc cttgtggatc
    18601 gccgttctct tggcgagcag gcgctggggg cgtttgaaga tacgcgtatt aacggcgaca
    18661 ctttcaacag cattttcgac attaaagggc tgacggataa attccccgaa gacagcacca
    18721 aaattcacgt tgccaccgta cagtcgctgg tgaaacgcac cctgcaatcg gatgaaccga
    18781 tgccggtagc ccgttacgac tgtatcgtcg tcgatgaagc gcatcgcggc tatattctcg
    18841 ataaagagca gaccgaaggc gaactgcagt tccgcagcca actggattac gtttctgcct
    18901 accgtcgtat tctcgatcac ttcgatgcgg taaaaattgc tctcaccgcc accccggcgc
    18961 tgcatacggt gcagattttc ggcgagccgg tttaccgtta tacctaccgt accgcggtta
    19021 tcgacggttt tctgatcgac caagatccgc cgattcaaat caccacccgc aacgcccagg
    19081 aaggggttta tctctccaaa ggcgagcagg ttgagcgcat cagcccgcag ggggaagtga
    19141 tcaacgacac cctggaagac gatcaggatt ttgaggtcgc cgactttaac cgtggcctgg
    19201 tgatcccggc gtttaaccgc gccgtctgta acgaactcac caattacctt gacccgaccg
    19261 ggtcacaaaa aacgctggtc ttctgcgtca ctaatgctca tgccgatatg gtggtggaag
    19321 agctgcgtac cgcattcaag aaaaagtatc cgcaactgga gcacgacgcg atcatcaaga
    19381 tcactggtga tgccgataaa gacgcgcgca aagtgcagac catgatcacc cgcttcaata
    19441 aagagcggct gcccaatatt gtggtaaccg tcgacctgct gacgaccggc gtcgatattc
    19501 cgtcgatctg taacatcgtg ttcctgcgta aagtacgcag ccgtattctg tacgagcaaa
    19561 tgaaaggccg cgccacgcgc ttatgcccgg acgtgaataa aaccagcttt aagattttcg
    19621 actgcgtcga tatctacagc acgctggaga gcgtcgacac catgcgtccg gtggtggttc
    19681 gcccgaaggt ggaactgcaa acgctggtca acgaaattac cgattcagaa acctataaaa
    19741 tcaccgaagc ggacggccgt agctttgccg agcacagcca tgaacaactg gtggcgaagc
    19801 tccagcgcat catcggtctg gccacgttta accgtgaccg cagcgaaacg atagataaac
    19861 aggtgcgtcg tctggatgag ctatgccagg acgcggcggg cgtgaacttt aacggcttcg
    19921 cctcgcgcct gcgggaaaaa gggccgcact ggagcgccga agtctttaac aaactgcctg
    19981 gctttatcgc ccgtctggaa aagctgaaaa ccgacatcaa caacctgaat gatgcgccga
    20041 tcttcctcga tatcgacgat gaagtggtga gtgtaaaatc gctgtacggt gattacgaca
    20101 cgccgcagga tttcctcgaa gcctttgact cgctggtgca acgttccccg aacgcgcaac
    20161 cagcattgca ggcggtgatt aatcgcccgc gcgatctcac ccgtaaaggg ctggtcgagc
    20221 tacaggagtg gtttgaccgc cagcactttg aggaatcttc cctgcgcaaa gcgtggaaag
    20281 agacgcgcaa tgaagatatc gccgcccggc tgattggaca tattcgccgc gctgcggtgg
    20341 gcgatgcgct gaaaccgttt gaggaacgtg tcgatcacgc gctgacgcgc attaagggcg
    20401 aaaacgactg gagcagcgag caattaagct ggctcgatcg tttagcgcag gcgctgaaag
    20461 agaaagtggt gctcgacgac gatgtcttca aaaccggcaa cttccaccgt cgcggcggga
    20521 aggcgatgct gcaaagaacc tttgacgata atctcgacag cctgctcgat aaattcagcg
    20581 attatatctg ggacgagctg gcctgacacg gtatcattcc cggcaatttc ttcttctgcg
    20641 gcccaataaa tttgggccgc tgcttaattt acggaacacc caatgaacaa taacgatctg
    20701 gtcgcgaagc tgtggaaact gtgcgacaac ctgcgcgatg gcggcgtttc ctatcaaaac
    20761 tacgtcaatg aactcgcctc gctgctgttt ttgaaaatgt gtaaagagac cggacaggaa
    20821 gcggagtacc tgccggtagg ctaccgctgg gatgacctga aatcccgcat cggccaggag
    20881 cagttgcagt tctaccgtaa cctgctggtg catctgggcg ccgacaatca aaagctggtg
    20941 caggcggtgt tccagaacgt caacaccacc attacccagc caaaacagtt gaccgaactg
    21001 gtcagcaata tggattcact ggactggtac agcggcgcgc acggtaagtc gcgcgatgac
    21061 ttcggcgata tgtacgaagg tctgttgcag aagaacgcga acgaaaccaa gtctggcgcg
    21121 ggccagtact tcaccccgcg tccgctgata aaaaccatta ttcatctgct gaaaccacag
    21181 ccgcgtgaag tggtacagga cccggcagca ggtacggcgg gctttttgat tgaagccgac
    21241 cgctacgtta agtcgcaaac caatgatctg gacgaccttg atggtgacac gcaggatttc
    21301 cagatccacc gcgcgtttat cggcctcgaa ctggtgccta gcacccgtcg tctggcgctg
    21361 atgaactgcc tgctgcacga tattgaaggc aacctcgacc acggcggcgc aatccgtctg
    21421 ggcaacaccc tgggtagcga cggcgaaaac ctgccgaagg cgcatattgt cgccactaac
    21481 ccgccgtttg gcagcgccgc aggcaccaac attacccgca cctttgttca cccgaccagc
    21541 aacaaacagt tgtgctttat gcagcatatt atcgaaacgc tgcaccccgg cggtcgtgcg
    21601 gcggtggtgg tgccggataa cgtgctgttt gaaggcggca aaggcaccga catccgtcgt
    21661 gacctgatgg ataagtgtca tctgcacact attctgcgtc tgccgaccgg tattttttac
    21721 gctcagggcg tgaagaccaa cgtgctgttc tttaccaaag ggacggtggc gaacccgaat
    21781 caggataaga actgtaccga tgatgtgtgg gtgtacgacc tgcgtaccaa tatgccgagc
    21841 tttggtaaac gcacgccgtt taccgacgag catttgcagc cgtttgaacg ggtgtatggc
    21901 gaagacccgc acggcttaag cccccgtact gaaggtgaat ggagttttaa cgctgaagag
    21961 acggaagttg ccgacagcga agagaacaaa aacaccgacc agcacctggc caccagccgc
    22021 tggcgcaagt tcagccgtga gtggatccgc accgcgaaat ccgattcgct ggatatctcc
    22081 tggctgaaag ataaagacag cattgatgcc gacaacctgc cggagccgga tgtattagcg
    22141 gcagaagcga tgggcgaact ggtacaggca ctgggtgaac tggatgcgct gatgcgtgaa
    22201 ctgggggcga gcgatgaggc ggatgcgcag cgtcagttgc tggaagaagc gtttggtggg
    22261 gtgaaggaat gagtttaaaa aatattccaa cgaattggtg tgtaactacc ataggggata
    22321 ttaatgacta taaatcatta acttttaatc ccaaatcata ccaggaggac agcttcgaat
    22381 tgtatagcgt tcccagcttt gcatctggct caccagaaat attactggga aaagatattg
    22441 cctccaataa acagtatatt cagtttaaag atattttatt atgcaaaatt aatccccgta
    22501 ttaatcgcgt ttggtgtgta aaagaaaaaa aagaacatcc tcaaatcggt tcatctgaat
    22561 ggattgtaat caggcaaccg ctcattcatg aaggtttttt ggcctatttt ttgcaaacac
    22621 aagatttcag agatctccta tgttctgatg tttctggtgt gggtggttct ctgacgagag
    22681 cgcaacccaa aagagtcgct acatataatg tccctatagc acctttgcca gaacaaaaaa
    22741 tcatcgctga aaaactcgat acgctgctgg cgcaggtaga gagcaccaaa gcacgtcttg
    22801 agcaaatccc acaaatcctg aaacgttttc g
//
LOCUS       DAZGCQ010000080        22442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000080 DAZGCQ010000000
VERSION     DAZGCQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.080"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            405..686
                     /gene="drpB"
                     /locus_tag="ACHK07_003435"
     CDS             405..686
                     /gene="drpB"
                     /locus_tag="ACHK07_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO8103721.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            726..1421
                     /gene="yedJ"
                     /locus_tag="ACHK07_003436"
     CDS             726..1421
                     /gene="yedJ"
                     /locus_tag="ACHK07_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO8103722.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALDVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1488..2906
                     /gene="dcm"
                     /locus_tag="ACHK07_003437"
     CDS             1488..2906
                     /gene="dcm"
                     /locus_tag="ACHK07_003437"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO8103723.1"
                     /translation="MQENILVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2887..3357
                     /gene="vsr"
                     /locus_tag="ACHK07_003438"
     CDS             2887..3357
                     /gene="vsr"
                     /locus_tag="ACHK07_003438"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO8103724.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDS
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHK07_003439"
     CDS             complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHK07_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO8103725.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4440..5357
                     /gene="yedI"
                     /locus_tag="ACHK07_003440"
     CDS             4440..5357
                     /gene="yedI"
                     /locus_tag="ACHK07_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO8103726.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5436..5618
                     /gene="yodC"
                     /locus_tag="ACHK07_003441"
     CDS             5436..5618
                     /gene="yodC"
                     /locus_tag="ACHK07_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO8103727.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSNGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPVEEV"
     gene            5861..7555
                     /gene="dgcQ"
                     /locus_tag="ACHK07_003442"
     CDS             5861..7555
                     /gene="dgcQ"
                     /locus_tag="ACHK07_003442"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO8103728.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7552..8367)
                     /gene="yedP"
                     /locus_tag="ACHK07_003443"
     CDS             complement(7552..8367)
                     /gene="yedP"
                     /locus_tag="ACHK07_003443"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO8103729.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8665..8892)
                     /gene="yodD"
                     /locus_tag="ACHK07_003444"
     CDS             complement(8665..8892)
                     /gene="yodD"
                     /locus_tag="ACHK07_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO8103730.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQHDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9055..9243
                     /gene="dsrB"
                     /locus_tag="ACHK07_003445"
     CDS             9055..9243
                     /gene="dsrB"
                     /locus_tag="ACHK07_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO8103731.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9287..9910)
                     /gene="rcsA"
                     /locus_tag="ACHK07_003446"
     CDS             complement(9287..9910)
                     /gene="rcsA"
                     /locus_tag="ACHK07_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO8103732.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10200..10985)
                     /gene="fliR"
                     /locus_tag="ACHK07_003447"
     CDS             complement(10200..10985)
                     /gene="fliR"
                     /locus_tag="ACHK07_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO8103733.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(10993..11262)
                     /gene="fliQ"
                     /locus_tag="ACHK07_003448"
     CDS             complement(10993..11262)
                     /gene="fliQ"
                     /locus_tag="ACHK07_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO8103734.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11272..12009)
                     /gene="fliP"
                     /locus_tag="ACHK07_003449"
     CDS             complement(11272..12009)
                     /gene="fliP"
                     /locus_tag="ACHK07_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO8103735.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12009..12374)
                     /gene="fliO"
                     /locus_tag="ACHK07_003450"
     CDS             complement(12009..12374)
                     /gene="fliO"
                     /locus_tag="ACHK07_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO8103736.1"
                     /translation="MNNHATVQSSAPISAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12377..12790)
                     /gene="fliN"
                     /locus_tag="ACHK07_003451"
     CDS             complement(12377..12790)
                     /gene="fliN"
                     /locus_tag="ACHK07_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO8103737.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADTVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12787..13791)
                     /gene="fliM"
                     /locus_tag="ACHK07_003452"
     CDS             complement(12787..13791)
                     /gene="fliM"
                     /locus_tag="ACHK07_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO8103738.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13796..14260)
                     /gene="fliL"
                     /locus_tag="ACHK07_003453"
     CDS             complement(13796..14260)
                     /gene="fliL"
                     /locus_tag="ACHK07_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO8103739.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14365..15492)
                     /gene="fliK"
                     /locus_tag="ACHK07_003454"
     CDS             complement(14365..15492)
                     /gene="fliK"
                     /locus_tag="ACHK07_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO8103740.1"
                     /translation="MIRIAPLITADVDNTTLSGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSTQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIAPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15489..15932)
                     /gene="fliJ"
                     /locus_tag="ACHK07_003455"
     CDS             complement(15489..15932)
                     /gene="fliJ"
                     /locus_tag="ACHK07_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO8103741.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAATRKPE"
     gene            complement(15951..17324)
                     /gene="fliI"
                     /locus_tag="ACHK07_003456"
     CDS             complement(15951..17324)
                     /gene="fliI"
                     /locus_tag="ACHK07_003456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO8103742.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISPE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17324..18010)
                     /gene="fliH"
                     /locus_tag="ACHK07_003457"
     CDS             complement(17324..18010)
                     /gene="fliH"
                     /locus_tag="ACHK07_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO8103743.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18003..18998)
                     /gene="fliG"
                     /locus_tag="ACHK07_003458"
     CDS             complement(18003..18998)
                     /gene="fliG"
                     /locus_tag="ACHK07_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO8103744.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18991..20649)
                     /gene="fliF"
                     /locus_tag="ACHK07_003459"
     CDS             complement(18991..20649)
                     /gene="fliF"
                     /locus_tag="ACHK07_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO8103745.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20864..21178
                     /gene="fliE"
                     /locus_tag="ACHK07_003460"
     CDS             20864..21178
                     /gene="fliE"
                     /locus_tag="ACHK07_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO8103746.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            21529..22442
                     /locus_tag="ACHK07_003461"
                     /pseudo
     CDS             21529..22442
                     /locus_tag="ACHK07_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
BASE COUNT         5422 a         5655 c         5851 g         5514 t
ORIGIN      
        1 tagggggtat caatgatggt tgcgccaaac acctggtgtg atcacgcgca cattaatgaa
       61 ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac gataaaaggc
      121 taataacgga ggcatcatga cacagaataa taaaaaggta taattgtatt cagtgctaat
      181 atattgatta aatgaattaa taaagccgat atcaaagata aataaaatcg ctcaaaacga
      241 tttttcctgt attcactgcc gttgcgcaaa aattatctat ttgttcaaaa aatgagtgag
      301 tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacataata gtactaagtc
      361 tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa gagagactct
      421 ctcgcagccc tggcggcaaa ctggctttgt gggctttcta tacatggtgt ggctatttcg
      481 tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc cccgtgtcgg
      541 gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt aggggcactg gtcggatttt
      601 tatttatggc tctggtcggg gcattgttag gcagcattgc ctggtacacc cgacctcgtc
      661 ccgcacgtag cagacgctat gaataaaaat tgctaaggtg gcgactcttt ggcaacggag
      721 cgcgtatgga cttacaacac tggcaggcac agttcgaaaa ctggttgaaa aaccatcatc
      781 agcaccagga cgcggcccat gacttgtgtc attttcgccg cgtctgggct acggcacaga
      841 agctggcggc agatgacgat gtcgatatgt tggtgatttt aaccgcctgt tattttcacg
      901 atattgtgag cctggcgaaa aatcatccgc aacggcagcg ttcttcaatt ctggcggcag
      961 aagagacgcg tcgcctgctc cgtgaagagt tcgagcaatt tccggcggag aaaatcgagg
     1021 ccgtttgtca tgccattgcc gctcacagtt tcagcgcgca aatcgccccc ttaacaacgg
     1081 aagctaaaat cgtccaggat gcggatcggc tggaagccct gggggcgatt ggcctggcgc
     1141 gcgtgtttgc cgtttcagga gcattggacg tggcgctgtt tgatggtgaa gatccgttcg
     1201 cacagcatcg cccgcttgat gataaacgtt acgcgctgga tcatttccag actaagttgc
     1261 tgaagctacc gcataccatg caaaccataa gaggcaagca gctggcgcag cacaacgcgc
     1321 aatttttagt cgagtttatg gcgaagctca gtgccgaact ggcgggggag aatgaaggtg
     1381 tcgatcacaa ggtgatagat gcgttttcac ctgcgggctg agcgcgtggc cctaaatggc
     1441 tgtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg caggaaaata
     1501 tattagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg gagaaactgc
     1561 tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa tggtgtgggt gagaatcact
     1621 ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc ggcatggcac cgtttaagtg
     1681 agaaagagtt cgcccatctg caaacgttgt tacccaaacc accggcacat catccgcatt
     1741 atgcgtttcg ctttatcgat ctatttgccg gaattggcgg tatccgtcgc ggttttgaat
     1801 cgattggcgg acaatgcgtg tttaccagcg aatggaacaa acatgcggta cgcacttata
     1861 aagccaacca ttattgcgat ccggcgacgc atcattttaa tgaagatatc cgcgacatca
     1921 ccctcagcca taaagaaggc gtgagtgatg aggcggcggc ggaacatatt cgtcaacaca
     1981 ttcctgaaca cgatgtttta ctggccggtt tcccttgtca gccattttcg ctggctggcg
     2041 tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatact cagggcacgc
     2101 tgttctttga tgtggtgcgc attatcgacg cgcgtcgtcc ggcgatgttt gtgctcgaaa
     2161 acgtcaaaaa cctgaaaagc cacgaccagg gtaaaacgtt ccgcatcatc atgcagacgc
     2221 tggacgaact gggctatgac gtggctgatg cagaagataa cgggccggat gatccgaaaa
     2281 tcatcgatgg taaacatttt ctgccgcagc accgtgaacg catcgtgctg gtgggttttc
     2341 gtcgcgatct taatctgaaa gccgatttta ctctgcgtga tatcagcgaa tgtttccctg
     2401 cacagcgagt gacgctggcg cagctgctgg acccgatggt cgaggcgaaa tatatcctga
     2461 cgccggtgct gtggaagtac ctctatcgtt atgcgaaaaa acatcaggcg cgcggtaacg
     2521 gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacgcgt acgctgtctg
     2581 cgcgttatta caaagatggc gcggaaattt taatcgatcg cggctgggat atggccacgg
     2641 gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg ttaacgcctc
     2701 gggaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc cgcattccgg
     2761 tttcggacac tcaggcctat cgccagttcg gtaactcggt ggtcgtgccg gtctttgccg
     2821 cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt cagcaagagg
     2881 cacaacatgg ccgacgttca cgataaggcc actcgcagca aaaatatgcg cgcgattgcc
     2941 acgcgtgata cggcgattga gaagcgcctc gccagtctgt taaccgggca gggcctggcg
     3001 tttcgcgttc aggactccag tctgcccgga cgcccggatt ttgtcgttga tgaatatcgc
     3061 tgcgtgatat ttacccatgg ctgcttctgg catcatcatc actgctatct gtttaaagtg
     3121 cctgcgactc gaaccgagtt ctggctggag aagataggta aaaatgttga gcgcgatcgc
     3181 cgcgatatca gtcgcttgca ggaactcggc tggcgcgtat tgattgtctg ggagtgcgcg
     3241 ttacgtgggc gcgagaagct gacggatgaa gcgcttagcg agcgtctgga agagtggatc
     3301 tgcggcgaag gtgccagcgc gcagatcgac acgcagggga tacatttact cgcttgatgc
     3361 gtcctgaata actggcgcaa ctacgggttt tgccggaaag agatattttc ccaacgtgac
     3421 cagtaccacc gcgaagacaa ttacgccgag cgccagccat tcaatcttcg acagtgtttc
     3481 tccacccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg cgtagctggt
     3541 ggcgagagcc ggactgacgt tgcggattaa atacatataa gcgttaatgg cgataatcga
     3601 accaaacagc gccagatagc cgaccgcaag gaagcctgaa agggaaggga gcgccgtcag
     3661 tttttcaccc gcaatcatcg acgcgatcat taacaccacg cctgccgcca gcatctcaat
     3721 cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaactg agccaaacgc
     3781 ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc ttaaatttcc
     3841 accgctattg agcatgatga ttccggcaag cccaatggca atacccaccc attccagttt
     3901 gcgcgtttta atgccaaaca ggcggctgaa gcacagggta aagaggggca cggttgcaac
     3961 cactacggcg gcgatgccgg aaggaacatt ttgatgttcg gcaaccgtca ccatgccatt
     4021 accgacagcc agcaataaca ggccaatcag cgcggcattg agcagcggac gtagcggggg
     4081 gagcttgtgt ccgcgcagta gcaaaaatgc cagcaataaa ataccggctg ccaggaatcg
     4141 aacgcccgcc atcattaatg gaggccagct ttccacgcca atccgaatga caaaataggt
     4201 tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaata actggcggaa
     4261 acgcataatc cctcacggtg gaaataaaaa ggtggttcat agtaaacgtg aaaatcattc
     4321 tgctggcgag agatataatt gcacttgatt gttaaaaaaa atgttgacct gtgaagcaag
     4381 tatcagagct gccgtttttg cttcatactt acacccttca acaataaaaa tgagagggaa
     4441 tgcttttggc cgggagtagt ttactgacgt tgctcgatga tatcgccaca ctgctggacg
     4501 atatctccgt gatgggcaaa ctggcggcga agaaaaccgc cggtgtatta ggggatgact
     4561 tatcgctcaa tgcgcaacaa gtttcaggcg tgcgggccaa ccgggaactt cccgtggtct
     4621 ggggcgtggc gaaaggatcg ctgatcaata aagtgattct ggtgccactg gcgctgatca
     4681 tcagtgcgtt tatcccgtgg gcgattacgc ctctgttgat gattggtggc gcgtttctct
     4741 gctttgaagg ggtagagaaa gtgctgcata tgctggaggc gcgtaaacat aaagaagatc
     4801 cggcgcagag tcagcagcgt ctggagaagc tggcggcgca ggatccgctg aagtttgaaa
     4861 aggacaaaat aaaaggggcg attcgtaccg attttatatt gtctgccgaa atcgtcgcca
     4921 tcacgctggg gattgtggcc gaagcgccgt tgcttaatca ggtgctggtg ctttcaggca
     4981 tcgcgctggt agtgaccgtg ggcgtctacg gtctggtagg ggttatcgtt aagattgatg
     5041 acctggggta ttggctggcg gaaaaatcca gcgcgctgat gcaggcatta ggtaaaggat
     5101 tattgattat cgcgccctgg ctgatgaaag cgttatcgat tgtcggcacg ctggcgatgt
     5161 tcctcgtcgg cggcgggatt gtggtacatg gtattgcgcc gctgcatcac gccattgaac
     5221 atttcgccgg gcagcaaagt gcagtggtgg cgatgatatt accgactgtt ttaaatctga
     5281 ttcttggatt tatcatcggc ggcatcgtgg tgctgggagt gaaagccgta gcgaaaatgc
     5341 gcggtcaggc acattaatac tcgcacaggc tattatgcaa aattcgccat cttcaactaa
     5401 ggtgaacaag tttcactcga aaaaaggagg cgagtatgag ctttatggtt agtgaggaag
     5461 ttacggttaa agagggcggc ccgcggatga ttgtcactgg atactccaac ggtatggttg
     5521 agtgtcgttg gtatgacggt tacggggtca agcgggaagc ttttcatgaa accgagcttg
     5581 ttccggggga ggggagtcgt cctgtggaag aagtttgaaa gggaatcatt aacaccaggc
     5641 cggatgtggc gctcacgtcg catccggtcg ggtgtgccag tgtctgcact gaattgcctg
     5701 atgcgacgct atcgcgtctt atcatgccta cgttataaaa caaaactatc gatgtctgtc
     5761 gcgtcttatc atgccaccat tataaaatta tcgctgtttt gcggtacgct atcgccaggt
     5821 tatgccagaa tcataaaaaa gcaggttggg agtcgtcagg gtgcagcacg agacaaaaat
     5881 ggaaaaccag agctggttga aaaaactcgc acgccgcctg gggcctggtc atgtcgttaa
     5941 tctctgcttt atcgtggtat tgcttttttc caccttgctc acctggcgtg aagtggtagt
     6001 gctggaagat gcctatatct ccagccagcg taatcatctg gaaaacgtag ccaacgcgct
     6061 cgataagcat ttgcagtata acgtcgacaa actgatcttt ttgcgtaatg gcatgcgcga
     6121 agctctcgta gcgccactgg atttcacctc tctgcgtgat gctgtaaccg agttcgaaca
     6181 gcatcgcgac gagcacgcct ggaaaatcga actcaaccga cggcgcaccc tgccagtcaa
     6241 tggcgtgtcg gatgcattag tcagcgaggg gaatctcctg tctcgcgaaa atgaaagcct
     6301 cgacaatgaa attaccgctg cactggaagt tggttacttg ctgcgactag cgcacaactc
     6361 ctcgtcgatg gttgaacagg cgatgtatgt ctcgcgtgcc ggattttacg tttcgacgca
     6421 gccgaccttg tttacgcgca atgtcccgac gcgttattac ggctatgtca cccaaccctg
     6481 gtttatcggc cattcacaac gagaaaatcg tcaccgcgcg gtacgctggt tcacttcgca
     6541 accggaacac gccagcaata ctgaaccgca ggttaccgtc agtgttccgg tagacagtaa
     6601 taactactgg tatggcgtgc tggggatgag tattcccgtg cgtactatgc agcaattttt
     6661 aagaaacgcc atcgataaaa acctcgatgg tgagtatcag ctctatgaca gtaagctgag
     6721 atttttgacc tcttccaatc ctgaccatcc aacagggaat atttttgatc ctcgtgaact
     6781 ggccatgctg gcgcaggcaa tggaacatga cacgcggggc ggcattcgta tggacagtcg
     6841 ctatgttagc tgggaacgtc tggaccattt cgacggtgtg ctggtgcgcg tccatacgtt
     6901 aagcgaaggc gtgcgcggcg atttcggcag tatcagcatt gcattaaccc tgctgtgggc
     6961 gctctttacc accatgttac tcatctcctg gtatgtgatt cgccggatgg tcagcaacat
     7021 gtatgttctg caaagctcgt tgcagtggca ggcgtggcac gacaccttaa cgcgtttata
     7081 taaccgtggc gcactgttcg aaaaagcccg tccgctcgcg aaattgtgtc agacgcacca
     7141 acatcctttt tctgtcattc aggtcgatct tgaccatttt aaagcgatta atgaccgctt
     7201 tggtcatcag gcgggcgacc gtgttctttc tcatgctgcc ggattaatta gcagttcctt
     7261 gcgtgcgcag gacgttgccg ggcgggtcgg tggtgaggag ttttgtgtga ttctgccagg
     7321 cgcgagtctg acggaggctg cggaagtcgc agaacgtatt cgcctgaagt taaatgaaaa
     7381 agagatgttg attgctaaga gtacgacgat acgcatcagt gcctcgttgg gggtaagtag
     7441 cagcgaggaa accggtgatt atgattttga acaactccag tcactggccg accgtcggct
     7501 ttatctcgct aaacaggctg ggcgtaatcg ggtattcgcg agcgataacg cttaacgggc
     7561 ggagaaaaaa tggtccagcc cttcacgcca tccttccggt ccttcacgct gcgttcgcca
     7621 gacgcgggcc ggatcctcat catgcagatg caccccttca cggtttagcc ctttcacaat
     7681 caccgcgtaa tccattacct ccagtaaggg cgcatcgttt ggcccatcgc ccaggccaag
     7741 tgtggttggg cgtttgcctg acaattgttg ataggtcgcg ataatccagt tggcagcctg
     7801 atcttttccg gcagaggcat ccagtacgtg ccagaagcgc gcaccttgca taaactgtaa
     7861 gcccagttcg ttcagacgag cggtaaattg tgccatacgc tcgtcactgt cgcgccaaat
     7921 tagcgttacc gacgcctcat gaagctgcgt cagcgccgcc tggctacggc ttaatcccgt
     7981 ccattcggcg atggttgcat cgtcgacatc atcaaaagtc gtgaatttaa aatgttcttt
     8041 ctcgcgtagc gtatttaaaa cctggctgat ttcgccatgg ctaatacctg agatgatgcg
     8101 tggaaaaccg tctatctcct gccattgctc agcaagctgg atcactgcgc cattttcggc
     8161 aatcagcggt aaaccttgta gccccaacgt tttttgcaag tacagcattt ccgctgatgt
     8221 tttactgcta cagagaatga cgggaacatt tgcttcgcgt aaacgggtga gccagggggc
     8281 tgccggttgc cagtcataac tatgactgtc cagcagggtg ccatcaagat cgctaaaaac
     8341 cagtagtggt tgttgaattg aaaacatagt tgcgcagtac tccccttacc aggatgattc
     8401 tcaaaacagc acaaattatc aaagatgatt ttttcgggag cgatcccgct gacgccagag
     8461 ccaccaaggg gtgacgtgcg tcacatttct attcataagt agcgttaatc attcatatgg
     8521 cgaatatttt cttgtcagcg aaaaaaattg cggataaggt gatgaacaca tcagatttcc
     8581 tggtgtaacg aattttttaa gtgcttcttg cttaagcaag tttcatcccg accccctcag
     8641 ggtcgggatt tttttattgt gcattcaacg attcacttca gagacgctga agtcatgaat
     8701 atccagttcg aaggcatccg ccaattcacg ccatgtatga tattcacgtc catcgacgct
     8761 aacgtgttca gaatcgtgct ggctgcgatg aacttcgctt tcgctaattt cattgatcgc
     8821 cgccagcagg gcatcgacat cgacgctgac ctcacgtttt gcggtatcgc tgtactcttt
     8881 tgcggttttc atcatctttc ctcgcaaccg ttactctgtt gttgccgcca gtcgttaagt
     8941 ccgcctggca tctcttaagt atagaccgtc gagaaaatca gcactcgcgc gcgcctggcg
     9001 ctgccagaaa aacgaaattg ttctacactg gcacaaagcc acaggaggaa aacgatgaag
     9061 gtgaatgatc gggtaacagt caaaacggat ggcggtccgc gtcgtcctgg cgtggtactg
     9121 gcagttgagg agtttagtga aggcacaatg tacctggttt cgctggaaga ctacccgctc
     9181 ggcatctggt tctttaatga agcagggcat caggacggta tctttgtgga gaaagcagag
     9241 taatcttatt cagcctgact ggtgggaaac caccagtcag aatgtgttag cgcatgttga
     9301 caaaaatacc attagtcaca ttatccgtca gtcggacgac atggtagata acctgtttat
     9361 tatgcgtttt gatcttacgt ttaatattac ctttatgcga tgaaacggtc ttggctttga
     9421 tattcatttg gtcggagatt tgaatggttc cctgacctgc catccacatt cgcaacatac
     9481 tcgattcggt tcggctcaat gataacgtcg gcatatttaa aaacgaggtt atcgttgtct
     9541 cttttttcag aatatcgcca aggatatcgt cgagagattc cggtttaatc gatttagaac
     9601 tgatcaataa attttttctg accaatagat attcatcaaa atgaacattg gcaattgcca
     9661 taaaaacgat aaataacgta ttgggatgtt gattaatgat gagcttgata cgctgactgt
     9721 tagaagcatc gtggatgaaa cagtcctcat taataaacac cactgaaggg cgctgtgaat
     9781 cacaagctat ggcaaggtca tcaacggttt caatgtcgtt gatttctctt tttttaaccc
     9841 ctctactcaa cagatacccg gttaaaccta gtcgggtgta actacataaa tccataataa
     9901 tcgttgacat ggcataccct cactcaatgc gtaacgataa ttccccttac ctgaatattt
     9961 catcatgact aaacggaaca acatgggtca cctaatgcgc cactctcgcg atttttcagg
    10021 cggacttact atcccgtaaa gtgttgtata atttgcctgg aattgtctta aagtaaagta
    10081 aatgttgcga tatgtgagtg agcttaaaac aaatatttcg ccgcaggagt atcctggaag
    10141 atgttcgtag gtattatttt tcggataatc cttaggataa catgataaac gttacggaat
    10201 tatattaatg gcaattcact aataatatca gccagcaaat taaacatctc actgaataaa
    10261 tgttcgcaaa aaggcgcaat taacggcatt aatgccgcca ttaaagagat accgacagtc
    10321 agagttaatg gaaatccaat aacaaaaatg gataattgcg gggccatacg attaagtaaa
    10381 cctaatgcca gattcagtgt cagcagcaga gtaatgagcg gtaacgccag catcagcccg
    10441 ttaaggaaaa tcaaactccc tgctttggtg agtgccagaa acgcattgct gttcaacggt
    10501 tcgccaccaa tcggcagggt gtgaaaggta tcgaccagca gtgaaatcaa ccataaatga
    10561 ccgttaaatg tcaggaacag cagtaacgcc agcatatcca tgatacgcgc taaaacgggc
    10621 atattgagat ggctggccgg atcgacaaac gtcgcaaatg acagccccat ttgcagaccg
    10681 ataatttcgc cagcggttcg cacagcggca aaggcaaatt gcatggtaaa accaagcgca
    10741 atgccgatca ggatctgctg cacggccagc cacagagcaa agaacgaaaa aacaggaaca
    10801 tcgttggcag gtaatgatgg ggcaatggcg aacgtgatca tcattgccag ccccagtttt
    10861 acccgtttcg gcacgctgcg ttcgctcaga attggtgcgg tagaaatgag cgccagcacg
    10921 cgcagtaacg gccagaagta caggcttaac caggatagcc attgttcgct tgtcacctgc
    10981 aacatagtac ggctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    11041 cagcaacaga ttgagcatcc acggtccggc aataataatg gcgataaata cggcgatgat
    11101 tttcggaata aacgacagcg tcatttcgtt aatctgcgtg gcagcctgca aaatactgat
    11161 gataaggccc gtgaccaacg ctaccaacaa tagcggggca gccagtgcca gcgcgacttt
    11221 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    11281 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    11341 cagtttaaag ggcagagcaa tggtggcggg gggaaccatc atcatcccca atgccatcaa
    11401 cacgctggct atcaccaggt cgataatcaa aaaagggatg aaaatcgtga agcctatctg
    11461 aaatgcggtt ttcaactcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    11521 ttcaggtccc tgcaacgggc cggtattcgc cagtctggca aacaacccta aatccgcctc
    11581 acgggtctga cgcagcataa actcacgcag cggctgcgcc cctttttcca gcgcctcctg
    11641 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    11701 cggtgacata ataaaaaagg tcaaaaacag tgccagcccc agcaatacct ggttaggtgg
    11761 cgcggagggt gttcccagcg cgttacgcaa taaaccaaaa acaataatga tgcgggtgaa
    11821 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    11881 ttgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    11941 cagttgcgcg aaggcgaggg gcgtaatcag ccagagaagg acaggtgcga cagacaataa
    12001 acgacgcatc aggatctccc gctacgctta agcaaatttt tcatgaccga ctgaaaatcg
    12061 gtctgcggta tctcttccgt tggtgcagaa gggggaagtt tatgcagcag attgatttgc
    12121 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    12181 cgcgcgccca gcgaagcact ggcactaatt ttcagaccgt taacgccagt tcgtttaggg
    12241 gcaaatccca gccgttttac tagccaggca gcagcgagga tcagggcaat aatggcgatg
    12301 agtgcgccgc tcacctgcag cagtggcgca gcagaaatcg gcgcggaaga ttgcacagta
    12361 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacggg gtaataatat
    12421 cggtgatccg cacgccatat ttatcagcga ctacgacaac ttcgccctgg gcgattaaat
    12481 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    12541 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    12601 gcttgaccgg aatatccata atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    12661 cgccaaattg ctggaacacc gtgtcggcag cgcttttgct actggttgat ttttgttcgc
    12721 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    12781 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    12841 tatccgtaac gcatactgac cgttgagggt gccgtactga ctggtcagca ccgggacgcc
    12901 gtcaacatgg gcgatgatgc gatcgggttt ttctatcggc aggacgtcac cgggtttcag
    12961 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13021 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    13081 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga aaggcaggca
    13141 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    13201 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    13261 aacttccagc ggattaatcg ccttccatgc gtcgctatag ccttcaagcg ccagtttcaa
    13321 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    13381 gaagcgtcca tcgccgccaa acaggttatc cacggcgata aacaccagac tcggcgagaa
    13441 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    13501 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggtaatatc
    13561 cgggctacgg cgcagcaggt tgaacagccc catacgaaaa tggcgagcaa agcgctcatt
    13621 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    13681 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    13741 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    13801 gcagaataaa agcggtatac agcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    13861 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    13921 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa gcgactacgg acttccggca
    13981 aatactcact cagccgcgag cgggtagctt catctttcag gcgcagggtt atgccgatat
    14041 aaagcacgcg atccgcatcg cccagattga ccgtaaaggt atccagcgca tagaagaccg
    14101 gtgagggcac gacgcgttgc tgcgctttgt cgtcagcggc aacctgatgc gaatgccagt
    14161 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggatccaaa
    14221 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    14281 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    14341 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    14401 acgcccttgt aaagagacgg gaaccggaag cgtatcgtcg tcttcccccg ccagaggttc
    14461 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    14521 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctttcggcca gctgcgtacg
    14581 cagtaccggc agcgctgctt ccagggcggc gcgtacatgc tgatggggtg aaaccatctg
    14641 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    14701 gtgcagacgc aactctgcac tttgttgccc ctggcgggtg aacagcgaaa tatgctggct
    14761 taatgattgt tgccattcgt gagaacccag cggtgcactc aaaacaggcg cggcgacggt
    14821 gggcagtggc tgtgtctggt gtggagcgat tagcgggctg gcggcagcgg tcaccggtga
    14881 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    14941 agctggcgtg ccgggggcgt catcaggctg tgctgttgtg agttgcgtag aagtcagttt
    15001 tgtgaagagc gttggtttct ctgtcggtaa cacggttgac ggcgcatcag tcactttggg
    15061 cgtattgtca aaacccggca acatcgcaaa aagggcactc aggcttgcgg tgacgtcttc
    15121 attcagatca tccgcttttt cgtcttttgc cgtattgttg ccagccaccg cagccaacgt
    15181 catcgtttga gcggtggtta atggtgttga tgtggattgt tcgtcgttga tgacaggcag
    15241 tgtttcatcc acggggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgac
    15301 cagcggctcg ccttttgtcg tgggcttatc tgttgccacc agcaactggg gagtcgcttt
    15361 gtcggttgtt gcctcgcctg ttaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    15421 atcgctggct ttgccgccag acaatgtggt gttatcaacg tcagcggtaa tcaagggcgc
    15481 tatgcgaatc attcaggttt cctcgtggcg gcgcgctggg cgaactcatc catctttttc
    15541 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    15601 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    15661 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    15721 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    15781 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacat
    15841 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    15901 ttcagggtcg ccagcgcgcc atgttctgtc atctgccgtt atctcctggg ttatgacact
    15961 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcgcg ttcaaaaatg
    16021 ccttgttgca aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    16081 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    16141 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    16201 gcgcggctga tcgacgcttc aatatcaatc gccggatagt gcccggcttc cgccagtcgg
    16261 cgagacagca caatatgacc gtcgaggatg gcccgcgcgg agtcggcaat ggggtcctgc
    16321 tggtcatcgc cttcggtgag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    16381 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    16441 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    16501 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    16561 cgcgtggcat aggcggcacc ttgcattcgc aggagcggag acacatccgc cggagcggca
    16621 atcaccactg atcgtgcacg cccttcggca ccgaggatat tctcaataaa atcttttact
    16681 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgggcc
    16741 atcatcccca gcagcacact tttaccgaca ccagaaccag caaacagacc catacgctgc
    16801 ccgcgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    16861 atcggtgtac gttgcaatgg gttaaatggt ggggtaatca gcgcaccggt ttccgtcgta
    16921 tcgggggagg gcaggccatc gagcggttta ccgctgccgt ccagaacgcg acctaataac
    16981 gccggaccga gcggcaactg tttgccgctt tgtagccctt caggcgaaat gtttttggca
    17041 taaacacgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    17101 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgc
    17161 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    17221 gtagcgcggg ttaatcgccc gtagcgacgt accgcaggca actgcgccat tttggcttca
    17281 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    17341 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    17401 cggcggagac tttacagccg ccaggatgga gggtgggatc gccccgcaag cgccagccat
    17461 gcaaacttaa ggtagcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    17521 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    17581 tcagtgccga gttatccact gttggcgtct gaccgatgac ctgacgtgcc gcctctagcg
    17641 ccatctgcat caggcgcgac gctatcacac tatcaagtgc atcaagggta gtttgaaatt
    17701 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    17761 gaccttgctc cagcccctga gccagtcctt cctgatagcc ctgcttatga ccttgctggc
    17821 gaccttcggc aatccccgcc tgataacctt gctcgtgggc ctgcatttgc agttgcgcca
    17881 gttgctgctc aaggctgggt tcggtctctt caatgatggt ttcttccggc tcaactattg
    17941 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cagggcagat
    18001 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    18061 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    18121 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    18181 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    18241 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattctc gaacaggaac
    18301 atctcgtcga tgattttctg cgccagttcg ccgtcgaatt cacgcacggc ggtaataacg
    18361 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    18421 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagctct
    18481 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    18541 agacgttcat cgaacaacgc cagaatatcg gcggcctggg cgcgcttcag atgcaccaga
    18601 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcgct ctgtggctcc
    18661 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    18721 aggctggcgg cacgttcttc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    18781 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatcg
    18841 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcacc
    18901 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    18961 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19021 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    19081 gacttctgcc cccagacgtt ggttagcgcg ccgttgttgg agttgttcgt ctttgctcag
    19141 gcggacttcc accgcatctt ccacttcctc gcgggcctgc gcctgttgct gtacagcttt
    19201 catcgcctca gcgcgacgtg ttaactgcgg gcgtaccgct ttacgccaca gcagccacgc
    19261 caccagcagt accagcaacc agcgaccggc agccagtaac tgatcgataa acgcttgctg
    19321 ttgccagaac ggcagttctc cgccgctttc ctcactacta ttgaacggcg agttaacgac
    19381 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgcc tcgcgggtca gatcttcaat
    19441 ttgcttcatc tgttcgttgc tgagaggcaa cggtttaccg tctggcaagg ttttgtaatt
    19501 caccacaacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    19561 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    19621 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    19681 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    19741 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcct gagattcatc
    19801 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gctgcgccgt
    19861 aacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    19921 ctgaatacgt ccttcgacat cgctggcata tttcaattga gcgtcattaa gatcgcgccc
    19981 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    20041 cggcagacca gcaacggcgc tggaaaccag atgcacaatg gcgctaattt gcccttcatc
    20101 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    20161 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    20221 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    20281 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    20341 tggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    20401 aatagcgccg ctggcttcgc tgaagcggta aggaatattc atttgcgtca gttggctgac
    20461 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    20521 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    20581 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    20641 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    20701 actcactccc gggtcagcaa actcgaaaaa ttgacgctcc cattatttgt gctttcgttt
    20761 ttttctatcc gccaataaac ccgttttttt gttgctattt agcgcctttg tcttattgac
    20821 ttactggtag gctttgctac cagaaattat ccaggagatg aaaatgtcag cgatacaggg
    20881 gattgaaggg gttatcagcc agttacaggc tacggcgatg agtgcgcgtg cgcaggaatc
    20941 actgccgcaa ccgaccatta gttttgccgg gcagctgcac gccgcgctcg atcgcataag
    21001 cgatacacaa acagccgccc gcacgcaggc agaaaaattc actctcggtg aacccggcgt
    21061 ggcgttaaac gatgtgatga ccgatatgca aaaagcctca gtttctatgc aaatggggat
    21121 tcaggtgcgt aataagctgg tggcggcgta tcaggaagtg atgagcatgc aggtgtaggt
    21181 tttgtaacct gttgttaatt acatccgatc tcaccgcgtg gggcatggat ggggcaaact
    21241 cactcaattt ctggtttaaa atggaaacct gattctgaaa gttttctgcc atccacgatc
    21301 cgtatacgcg ataaacctta tgatcatcag aatgtctcat ttgacacaca tcaccacgtc
    21361 ggatgttgtt acgaccacag acgcaacagt aatctctgga taatttaatc ttcgttctta
    21421 tttttattaa tatactgcgt tataaaaatg agcgattttt gatagtggta attttattgt
    21481 gttttagaat gctccctggg caatataatt tattaaacag gagtgtaaat gtttccatta
    21541 aatgatcttt cgttaaaaac tcagccggtt caattaaata aagtcacatc taatactgaa
    21601 tctacgataa aacaacatga gttagtatct gatgatgcaa tcataaatga attatcaagt
    21661 gagttagtca gttgtcttgg aaatggtaag tttacaccaa ttagtgaaga cagcaaatta
    21721 tttaatatgc tgtcagaatt taagttattg cactcagtat atttcgaatg gggtgattat
    21781 agtctctggt ttcaagattt ctcgatttat aataagatag gttttatcat gatagaaaaa
    21841 atcagggaac tggtaaccca cccattcggc ataaattaga gtttatatcg actaatattg
    21901 cagagtttct agacaacttt acgaaaatta cagattccag actttgcaaa ggtttcagtg
    21961 actgggctaa ttcagtcaaa gaaggcgcat cgaatgactt caaaaaaaat gtggatatag
    22021 cattggtgag attgtttaaa tgtgttgaac tccacaatag taaacttgac ttaacagatc
    22081 ttcacttggg gtctttgcca cctcttccat cctggattga ggtgctttat cttcgtcata
    22141 atggccttgc cacaatacag gctcccaaat tctgcaaaga attagagctc gatttcaata
    22201 attatatggt atttccaaaa gtatccgatg gaattaccca ggtctccgta gataataatc
    22261 tgatatcgcg tgttgactca tctccgtcaa aggctatgac aatttctatt tatcgcaata
    22321 aaatatggta aataccagca atgcctgaga ctactaagct gtttaattgt ggcgataata
    22381 agataaaaga aattcaatac ttcccaaatc atctggagaa agcacgtatg gagtgtgata
    22441 tt
//
LOCUS       DAZGCQ010000081        22424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000081 DAZGCQ010000000
VERSION     DAZGCQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.081"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            273..4085
                     /locus_tag="ACHK07_003462"
     CDS             273..4085
                     /locus_tag="ACHK07_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO8103747.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNSATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGLLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4174..5793
                     /locus_tag="ACHK07_003463"
     CDS             4174..5793
                     /locus_tag="ACHK07_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO8103748.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQLKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            5809..6177
                     /gene="acpS"
                     /locus_tag="ACHK07_003464"
     CDS             5809..6177
                     /gene="acpS"
                     /locus_tag="ACHK07_003464"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO8103749.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIEQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6191..6952
                     /locus_tag="ACHK07_003465"
     CDS             6191..6952
                     /locus_tag="ACHK07_003465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO8103750.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHILQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            6956..7504
                     /locus_tag="ACHK07_003466"
     CDS             6956..7504
                     /locus_tag="ACHK07_003466"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO8103751.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAHVGKKLISQGTIINFRETK"
     gene            7526..7807
                     /locus_tag="ACHK07_003467"
     CDS             7526..7807
                     /locus_tag="ACHK07_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8103752.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            7843..9003
                     /locus_tag="ACHK07_003468"
     CDS             7843..9003
                     /locus_tag="ACHK07_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO8103753.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCNID
                     GYYLLSEDIPAAELIASMNTILEKAEELDIQSLPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9077..11635
                     /locus_tag="ACHK07_003469"
     CDS             9077..11635
                     /locus_tag="ACHK07_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO8103754.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHLELGSVT
                     FPYDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALKHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            11640..12863
                     /locus_tag="ACHK07_003470"
     CDS             11640..12863
                     /locus_tag="ACHK07_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO8103755.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHAAKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAITMFAGERQILVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNTQAGRAK"
     gene            12860..13813
                     /locus_tag="ACHK07_003471"
     CDS             12860..13813
                     /locus_tag="ACHK07_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8103756.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERRQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            13824..14531
                     /locus_tag="ACHK07_003472"
     CDS             13824..14531
                     /locus_tag="ACHK07_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8103757.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14515..15171
                     /locus_tag="ACHK07_003473"
     CDS             14515..15171
                     /locus_tag="ACHK07_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103758.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVKQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVSEWDSEELTQVLLPLMKGE
                     "
     gene            15172..16482
                     /locus_tag="ACHK07_003474"
     CDS             15172..16482
                     /locus_tag="ACHK07_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8103759.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSTKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16491..17279
                     /locus_tag="ACHK07_003475"
     CDS             16491..17279
                     /locus_tag="ACHK07_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO8103760.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17276..18664
                     /locus_tag="ACHK07_003476"
     CDS             17276..18664
                     /locus_tag="ACHK07_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103761.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMNLDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHHETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLIDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFSLFGD
                     TLGLYLGFKYYL"
     gene            18678..19619
                     /locus_tag="ACHK07_003477"
     CDS             18678..19619
                     /locus_tag="ACHK07_003477"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO8103762.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFTDAKVFK"
     gene            19616..20602
                     /gene="ycdU"
                     /locus_tag="ACHK07_003478"
     CDS             19616..20602
                     /gene="ycdU"
                     /locus_tag="ACHK07_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO8103763.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            20969..22171
                     /locus_tag="ACHK07_003479"
     CDS             20969..22171
                     /locus_tag="ACHK07_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8103764.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5830 a         4972 c         5871 g         5751 t
ORIGIN      
        1 ttcagatatt cgctgaattg gattttttct cacccaggga gaaggggtgg ggacttttaa
       61 attattgtgt attagtaagt taggtacgtc tccgttcgaa gaaaaaacaa taactcttag
      121 ttgttttttg agtccttatt tcagcttatt tttcgtgatt ttttattgcg ccaggtgata
      181 ggatttgccg caatcatttt tgttgatatt ccgcaaatca tcaaaagtga tctgtatgtc
      241 caaaatgaat agcatcaata aggaaaacgt tagtggcgat gataaattta agtaaggaag
      301 caacggtggg taaagcatta acccctattg ctatacttat gatgttgtct tttcctgtag
      361 cttctcaagc ggcgggatta gtcataaaaa atggaacggt atataacgcc aatggtgtgc
      421 cagtcgttga catcaacaaa cctaacggta gcggtttatc tcataatatc tgggataacc
      481 taaacgttga taaaaatggt gtcgttttca ataacagcgc taatgaatcc agtacttcac
      541 ttgccggaaa tattcaggga aacagtaatc tgacctccgg gtcggcgaag gtgatcctga
      601 atgaggttac ttccaaaaat ccttcaacca ttaatgggat gatggaagtt gcaggggata
      661 aagcggatct gattattgcc aacccgaatg gtattactgt aaacggtggc ggttcaatca
      721 atacaggtaa actgacctta accaccggga cgccggatat ccaggatgac aagctggccg
      781 gttactccgt gaacggcggt accattacgc tcggtaaact ggataacgcc agcccgacag
      841 aaattctgtc ccgtaacgtg gtggttaacg gcaaagtgtc tgccgatgag ctgaacgttg
      901 ttgctggcaa taactatgtt aatgccgcag gccaggtgac cggtagcgta tccgccacgg
      961 gttcccgtaa cggttacagc gtagatgttg ccaaactggg cggaatgtat gcgaacaaaa
     1021 tcagtctggt cagcaccgag aaaggtgttg gggttcgcaa cctcggcgtt attgctgggg
     1081 gggttaatgg tgtcagcatc gattccaaag gtaacctgtt aaacagtaac gcccagattc
     1141 agtctgcaag cacgatcaac ctgacaacaa atggtactct ggataacacc accggtacgg
     1201 tgacatctgt aggcactatc tcgctcaata ccaacaagaa tactatcgtg aatacccgtg
     1261 cgggtaacat ctctacgatg ggtgatatct acgttaacag tggcacgatt gataacacca
     1321 acggcaagct cgccgcagca ggtatgctgg cggtcgatac caatagcgcc acgctgatta
     1381 actcaggtaa agggagttct gtcgggattg aagcggggct cgtggcactg aaaaccggaa
     1441 cgctcaacaa cagcaatggt cagattcgcg gtggctatgt gggtcttgaa tccgctgcgc
     1501 tgaataacaa caacggtgat atccagacca ccggcgatat cgccattatc agtaacggta
     1561 atgtggataa caacaaaggt ctgatccgtt cgtccaccgg gcatatcgtt attggcgcgg
     1621 caggtagcgt aaataatggt tcaaccaaaa ccgccgatac cggcagttct gactctctgg
     1681 gcattattgc agataccggc gtagaaattg gtgcgaacaa catcaataac aacggcggac
     1741 agattgcgtc taatggcaac gtctccctgt caagttacag cacgatcgac gactatgcgg
     1801 gcaaaattct gtccaacagc aaagtgatta tcaagggaag ctcactacgt aacgataccg
     1861 gggggatcag cggtaagcag ggcattgaag tcgccgttgg cggcagcctg accaataaca
     1921 ttggcgtgat cagctctgaa gagggtgata tctccctgtt agccaactca gtggacaacc
     1981 acggcggctt catgatgggg cagaacatca cgatggagtc gatgtctggc gtcaataaca
     2041 acaccgcgct gatcgtggcc agcaaaaaac tgaagataaa cgcgttcggt aatattgaaa
     2101 accgcgatgg caatagcttc ggtaatgcat atggtctgta cttcggcatg cctcagcaaa
     2161 cgggtggaat ggtcggcaag gaaggcatcg agctttccgg gcagaatatc tataacaaca
     2221 acagtcgttt catcgctgag gatggtcctc tgactctgca ggcacagaat acgttcgaca
     2281 acacgcgtgc tctggtcacc agcggggcgg atgcatctat tcaggttggc ggaacgtatt
     2341 acaacaacta cgctaccacc tggagcgcgg gtaacctgga tatcgacgcg accacactgc
     2401 aaaacagcag tagcggtacg atgatcgata acaatgcgac cgggttcata gcatctgata
     2461 aaaacctgtc actggaagtg gtgaataccc ttaccaacta tggctggatc agcggtaaag
     2521 gtgatgttga tgtcacggtg aataacggca acctgtacaa ccgcaatact attgcggctg
     2581 aaaaggggct ggacattgca gcgctgaacg gtattgaaaa ctggaaggat atttctgctg
     2641 gcggcgacct gacgatgaac accaatcgcc atgtgaccaa caactccaac agcaatatgg
     2701 tggggcagaa tatcgttatt aacgcggtta acgatatcaa caaccgtggc aacattgtca
     2761 gtgacgctga cttgaacgtg acgaccaaag gcaacctgta taactatctc tatatggtag
     2821 ggtatgggga tatcgcattg tcggcaaata gcgtggcgaa caataacgcg accatcgaag
     2881 cgacaggcga tctgattatc gattcgaagg gtaacgtggg taacaaccgc ggtaatctgc
     2941 atgcgttgaa cggcgtgttg tctgttaaag gcaacaatct gaacaacgat aacggtgaaa
     3001 ttcgtggtta tggcgatgtc acgctggcac tgacgggcaa ctacgacagc tataagggtt
     3061 tgctgacctc tgaaacgggt gacgtgactc tgacggcgaa catcgtagac aacgcctatg
     3121 gtttgattgc cggtgagaat gtttctgtcg atgctaaatc gacgatttac aacaacactg
     3181 cgctgatcgc ggcgaataaa aagctggtta ttaacgctgg cggcaacctc gaaaaccgcg
     3241 acgggaataa cttcctgcgt aataacggcg cgctgtttgg gatcaccgac aacgttggcg
     3301 gcatcgtagg taaagaaggt gtcacgcttt ctgctcagaa cgtctacaac aataacagca
     3361 gcatcatcgc tgaaaatggt ccgcttaatc tgctgtccag gggaacgctg gataataccc
     3421 gcgcgcttct tagcagtggg gctgatgcca tcatccgtgc ggcagggacg ttctacaaca
     3481 actatgccac cacgtacagc gccggtaatc tcgacgttta tgcggcgtcg ttgaacaacg
     3541 ccagcgatgg tcgcctggaa gacaataccg ccacgggcgt gattgcgtct gacaaaaacc
     3601 tggatctgag cgttgataac agcgtcacta actatggttg gatcagcggt aaaggagatg
     3661 tgcatttcaa tgttctgaaa ggcacgctgt ataaccgtaa tgccatcgcg gcggacaacg
     3721 cgctgaccat taatgccctg aacggtgttg agaactttaa agacattgtg gcgggtactg
     3781 cgctgactat tgatacgcag aagtatgtta ccaacaacag caacagtaat atgttgggac
     3841 aaaccatcgc gatcaatgcc gtgaatgaca ttaataaccg cggaaatatt gtgggtgatt
     3901 attctctggg tgttaaaacc accggtaata tttataacta cctcaatatg ctgagttatg
     3961 gtgtcgctgg cgtatcggca aataaggtta cgaatagcgg taaagacgcc gttctcggtg
     4021 gcttctacgg tttagcgtta gaagcaaacg aaactgataa caccggtact attgtcggca
     4081 tgtaagatgt aaccaaagac agcagtggct tttaatatta atgcgtcatg tcacgcaggg
     4141 gttgctgctg tctttattta gtttgagaag ataatgcaat tacgatttaa ttttggtttg
     4201 ctgctcattc tttgttgttt tcaggttgca gcgaaagaca gtcttgataa tttgatcaag
     4261 cagcaaaaaa atgcagatga aagcacgttg cgcgcgcata aattagaaaa aaaagatgtt
     4321 tactcaaatg cacaacgtaa tgcgtttact ctgggtgatc tgccgcagga aaagaattgc
     4381 tttatcatca atcggattga actggaagat aactttctgg gtggtcagtt gatttctgaa
     4441 gtgcaaaaag cgattgccgg acgttgtgtc ggtagtgctg gtgtgcaaaa aattgctgct
     4501 gccttgcagg attattatat taatgccgga tatatcacaa cgcgaattag tatcccttcg
     4561 caggatattt ctaaaggcaa gctacgtttc caggttaatg ccgggaagat agaaaaaacg
     4621 attgtcgaag gtaatgatat cagtgaatgg atgttacctt ttcagcgtaa cgatattctc
     4681 aatattcgtg atattgaaca ggggctggaa aatcttcagc gagtacctga cgttgatgtg
     4741 aaaattaata ttgaaccggg aacacgggat ggttacagca tcgttcatat tgatacacac
     4801 cgggggaaaa actggagcgt tcgggcaagt tacaacaatt ggggcgatga agaaacgggc
     4861 cgttaccaga caaccgcggt gggttatttg tttaatccag cgaaaatggg ggatctgttt
     4921 tatctcgccg gtacgcgtag tactaccgga cagtatgaaa acgtcagtag ttattattcc
     4981 attccggtgg ggtactggga gtacggcttt ttctatagca aaagtaaatc gcagcaggtg
     5041 attccgcttt cgtacatctc gctggattat gttggcacca gcgaatactt gagcgcaaaa
     5101 gcgacgcgta ccgtttatcg cgataaaaca aaaaaattcg ccggtagtgc agaacttatt
     5161 cgtcgaaagt cgaattacac gcttaatggt gaggagctga cattacaggc gcgggatatg
     5221 ggaaatatca agctggggct aaattataag caacagttac ccggcgcgtt ctgggattca
     5281 accctctcgt ggcaacgctt tttaacctgg ttcggtggtg aaaaaacgcc ggatatgcaa
     5341 tatggcgacg ttagcccggt gagcaatatc tttaattttg agggtaacta tacccgacaa
     5401 ctaaagaatg ggtattacaa tactgctttt tttgcccagt acgcaccgcg tgaactgact
     5461 ttgcaagatc aaattacagt tggcgatcgt tggagtattc gcgggtttga aaatagcgta
     5521 ggtttgtcag ggaatgatgg tttttatata aaaaatacgc tggcatttcc tttgcctgga
     5581 atgaaagcta actattatgc cggtcttgat tttgggcaag tgtatcagga cgcttcttat
     5641 ggcgatgaat cactggtggg cgcggcagtt ggtattgatg gaaatattaa aagtcttgag
     5701 tataactttt ccgtttctac cccattaaag tatccggcga cattagatat tgatcgggtg
     5761 aatgtaaatt ttaatttttc gtaccaaatg taaaggataa cgggactcat gagaataggg
     5821 acagatattg ttgaaattgc cagaataacc aaagccatta ttaatggaga gatggctttt
     5881 tatgagcgtg tttatactgc tcatgagcgc ttactcatta accctgcaga acctgattat
     5941 gaacgggcct ctggattctg ggcggcgaaa gaagcgttag ttaaagcgat tggttgtggt
     6001 tttcgtgatg gcgtacgttt ccaggatatt gaaataaagc atgatgcagc caaagcacct
     6061 tatttcgtta tttccggaag actacaagaa atttttatcg agcaggggtt gcaaaataac
     6121 tccctgagta tttcgcattg ccgtacccac gccattgcga ctgtcatcgt ttattaactc
     6181 aacatatgtg atgaatatgt acaaatataa tgatttaaat gaaaaaaccg tactggtgac
     6241 aggagccagt ggtgatattg gactgggtat ttgcgaaaaa tatctggagc agaactgtga
     6301 tgtttatgcg ctctataaat ccaacgacgt gcaattaacg gcgctgaaag caagccatcc
     6361 tgcaggcgat aaactgcata ttttgcagtg cgatctcgcc tgcccccaga gcgtgagcgc
     6421 attgtgcgaa cagatagagc gcgaagctgg caagattgat gtgctggtga ataacgcggg
     6481 catcgttaaa gacagcctgt ttgcctcgat gagctatgag gatttcaccc aggttatcga
     6541 aaccaacatg ttcagcattt tccgtctgac gaaagacgcg ctgatgttgc ttcgtgcggc
     6601 agaaaatccc gccattatta acgttgcctc tattgcagcg ctgatcccaa gcgtcggtca
     6661 ggcgaattac agcgcgtcaa aaggggcgat cctaggcttc acccgcacgc tggcagcaga
     6721 aatggcaccc tggggcgtgc gcgtcaacgc ggtcgcaccg ggaatgattg aatcgaaaat
     6781 ggtgaaaaaa gtatcccgcg cagtggtccg tgcggtaacc agtaccatcc cgttacgccg
     6841 tctggggaaa tgtgaagaag tggcgaacac catcgtcttc ttaagttcct ctgcctccag
     6901 ctacattgtc ggccagacca tcgttatcga tggcggcctg gtcatgcggt gagttatgtc
     6961 taaatacacg attccttcga aaatcttcct cgaaatgggc ggctggcgtc agccgctgct
     7021 gatggtcgat aaaattgcag attataaata cggtgaaaat ggctttgtca gtgtcgtgaa
     7081 acacgtcacc tacaacgaac cgtatctgct ggggcatttc ccggaagatc cgataatgcc
     7141 cggggtaatt atttcggaaa tcttcggcca ggccagcgag tatctctctt tcctgaccga
     7201 catttgcgac atctggcgcg aacgttttga agaagagctg aagtcgttac gtgatattca
     7261 cgcacgcatt caccgccctg aaatgctgga gattatccgt acccggcgca gccaggtacg
     7321 cggcgttctg gcggctcaga acctgaaatt taaagatatc gcctatccgg gtgattctat
     7381 tgatgttgtc agcaaattgg ctttttcgga tgccagtggt tttaagcact attccgtgac
     7441 cgcgcatgtc ggtaaaaagc tgattagtca gggcaccatt attaatttcc gtgaaaccaa
     7501 gtaatccaat aagagagtaa acactatgtc taccatgaat gacgctattg aacagcgtaa
     7561 agaagttctg atgaccatta aaactgagat catccagcga ctgaatattc aacgcgatga
     7621 agcgcaaatt gatgatgata ctgcgctgtt cggtaatggt tttaaactgg actccgtcga
     7681 tgccacggaa attgtcgtcc tgctggataa agtttttgat atcaagatca gcgaaagtga
     7741 tgacccgtca tatatgcgca gtatcaatag tctggccacc ttcgttattc aaaagaaaaa
     7801 cgactaacta ctttttaaag aattaaatat caggacacgt ttatgaaatc tcttgctgaa
     7861 tttcacttga aaaataatgc ggtaatgggt gtgtataaca accgtaccct accttcttct
     7921 tatcacgatg ctatgactga atataaagct gtacgcgaaa acgcgctgct ggtggactat
     7981 tctcacctct ctatcgttag cgtcatgggc gatgatgcct gggcgctgat caaccagctg
     8041 gtttccgcag atgtctccat cattcgtgat gagcaggcaa tctactcact ggtgttgaac
     8101 gaagagggca ccattcgcgg agacgtctat gtgctgtgca acatcgacgg ttattacctg
     8161 ctgtctgaag atattccggc tgccgaactt atcgccagta tgaataccat tctcgaaaaa
     8221 gcggaagaac tggatattca atcgctgcct gaaattcagg atatgcggga aaacaactgg
     8281 ggggcaatcc tgctcgaagg cccatactcc tgggagatca tgtctgaaat tcatggcttt
     8341 gacgtgattg gcctgcctta ctacgaatac atgaataccg atgaggatct gctgatcttc
     8401 cgctgtggta aacacggtga gtatgcgtac atgaccatcg gtgagcaggc gaaactggcg
     8461 gagcagtggg aaaaactgtt aaccgtcggt gagaaatacc tgatgcagac cggcggtctg
     8521 gattatcaaa aaatcgtacg cctcgaaaat ccgtgctggg atgccagcct gtgggaaggt
     8581 cagtcggtga atccggttca gttgcagatg cagtgggcgg tgcagtacga caaagatgat
     8641 tttattggca aggacgcggt gacagagctc tcccaggagt ataccggtaa taaactcatc
     8701 ggcatgatcg ctcaggaaga atgcgaaggc attgaggctg gcgatcgtgt gctggtggaa
     8761 ggtcaagacg tgggctatgt cgtgaaagcg gtattttccc cggcattgca gcgtttcatt
     8821 gccctgaccc tgctggaaaa ggattacgcc tggtctgata tcagcggcta cgaaattcaa
     8881 actgcccacg gaattattcc ggcgcaatca aaatgtatgc cgtttattta taacctgagc
     8941 atgttggtta gcccaactga gcacagttat atcgacgcgt caaaaaataa aagcgcagcg
     9001 taatgaatat cgccgcgtga ctatagctta cgcggcttaa attaacccca attggcggga
     9061 ttgccaggta ttattcatga aaacgaacag gaaaagagtt gttattaccg ggctggggat
     9121 tttgtcctca ctggcggaaa atattcagga tttccgggaa gcattattaa ataaaaaaaa
     9181 tggcgttgcg gatagcgaac gctttgcaaa atggtttgaa aatgcgcgtg cggcagaagt
     9241 gttgcatgac attgaatact ccgatctgaa cgatgatgtt atcgattcac tggataacgc
     9301 agcgctctgg gcctacaaag tgggtaaaga tgcgctgagt caggccggcc tgatggatag
     9361 ccgtgacacc ctcaaagaaa caggcatgat cgtcggtgtt tcctccgcgg gtacagaggc
     9421 ctttttaccg ctgttcgaac agcgcatgga agatttctcg ctgcgtaaag cactcttttc
     9481 cggcgcattc tcatcctgtt gttccagcgt ttcaacgctg ctgggcttac aggggggggt
     9541 tgaactggtc gcgaccgcgt gtaccgccag cccgaacgcc gtggggatgg cctttgacta
     9601 catccagaat ggtaaaagca aaaccatgct ggcagtaggt accgagccca tctatctgcc
     9661 gacctttgcc ggattctacg cactgaatgt catgcatcca gacacttgta cgccgttctc
     9721 gggtcagtcc ggtatgtcga ttggtgaagg tgcgggcgct atcgtactgg aagagtatga
     9781 gcatgcagta gcccgtgggg cgaccattta tggtgagatc ctctcttacg ccacttcctg
     9841 cgatgctttc catgagaccg ggccagatcc gcgtgccagc ggtgcggtgc aggtcatgaa
     9901 caaggcaatg gcaaatgcgg gcgtcacacc ggagcaaatc gactacgtca acgcgcacgg
     9961 tacgggtact gaagccaatg accgtatcga aacgctggca atgaaaaagg tctttgctaa
    10021 ccacgataaa ctgctggtga gctccaccaa atcttacttt ggccacaaca ttggcgcagc
    10081 ggggattgtg gaactgattg cctgtctggc aacactgccg gacaaccgcg tactgccgac
    10141 gctgaatttc agccaggcac gaccgaattg cgatctggac tatgtgccga atgaatttcg
    10201 cgatcgcaaa gtgaacatct tcatgaagaa taactatgcc ttcggcggca acaactgctg
    10261 catgatcctg agcatggagc ccgcgtccat tccggtcagt acctatgatt cgaagcgtgt
    10321 ggctatctcg ggcgtcggtg cagtatccgc aattggccac acgctcaacc aggttctgga
    10381 aaacatctgg gcgcaggacc accaccttga actgggtagc gtaaccttcc cgtacgatac
    10441 actggaagaa gccaaagagc tgctggccgt gctggatgaa tcaaaccagt ttgccgagct
    10501 gtttgatgaa gagtacagct tcagcgacgc tacgcttccg gaaacggcaa gcaacttcaa
    10561 aacgttccag gtttccgggc tggagccgcg taaacacctg cgtcgtttcg atcagcgtaa
    10621 agcaacccgt ggcggcacgt ttgccctgat cgcactcacc gaagcgctgg aacaggcgaa
    10681 gcgtaaaatc aaacgcgacg gcgatgagct gggcatgatc atggggatgt cccgcggccc
    10741 gcaggaaacc acctacaaat atctgcaaag cctgaaaccc gatccacgca aagtacgtac
    10801 gtcggaattc cctggctcac tgatgaatgc gatcccaacg ttctgcggga tctctgaagg
    10861 gatcaaaggg tataccacta cgcttgcgac aggggaaaac tcggcgctgg gcgcactgac
    10921 ctacggttat gaaatcgtcc ggcagaacct gcaaccgcag gtattggttg gcggcgcgga
    10981 tgagtacttc ccgtcgatgt cgctgtatat ggatgcggtg acacaaaaga tccacatgac
    11041 ggctgaggcg agcgactatc aaatttacgg taaagatccg cagggttatg ttccgggcga
    11101 gggtgcctgc atgctgctgc tggaagatcc acagttggct gcggcgcgtg gcgctgaggt
    11161 tctcgcggaa gtggtgggct acggtaagtc atgtagtaat acctacttcg acgcctcatt
    11221 gttggaagaa aaatccgcgt cgatggcgct ggccattgaa cgcgccctta agcatgcggg
    11281 catctctgct ggcgatattg atttggtatg tggtaccagc aacggcagcg atgaccattc
    11341 tcgtatcgaa attgacgcca tttacaacac cttcgccgcg caaaacccgg cggtgccggt
    11401 agtgaactac aacgcttgct ttggttttgt cgcctctgcc gctggtttgc ttaacctcgc
    11461 cgtgattact gactgcatta agaagcaggc agtacctgcg attccaaata cggtggaatt
    11521 ctttgatgag cgtgtgaatt ttgtacgcca gtcgatgaaa cttgcgctga accatgtcct
    11581 gcttgttggc gcaacggaag gcggcaacta ctacgcgttc gtcattaagg gataagactg
    11641 tggacaatgc cgctattatt tcgggttaca gcgtgtggat gccgtatgcc gaagactgtt
    11701 cgcagttaat tgacaatctg aaacagggga aacgcgttgc gcgtacaccc tggtttacct
    11761 ccaacgagga agccattaaa tgcgggttta agggtaatcc gtcagtggcg acgttgaaac
    11821 aggtggacga tagcgcattg gacctgctgt ctcagttgat cgacgaagcc ctggaacagg
    11881 caatgctgga taagcattgc ctggccgggc gcaacgttcg cgtttacctg accggtattg
    11941 ggccgcgtat cgacgggctg gattacaaat ctttctataa ttacaacgat gtagaagata
    12001 tcaacttaac gcaatccatc accaatctgc atgccgcgaa gatgtcgcag gacacgattt
    12061 caagccatct tgcgcgcaaa tatcgcttgc agtatttgcc gcctaacatg aactgcacca
    12121 gtaactcgtc actgactgcg gtgcatctgg cgacgcaggg cattgagcag ggcgggattg
    12181 atctcgcgat tgtgctgaac tgttcgaaaa ttaaaaccca ggatatctgg ttcctggaaa
    12241 ctcaatcgat gctggacagc gaacaggttc agccgtttgg tgaaaacagt aaaggtgtgg
    12301 cgttcgcaga aggttttagc gcgctcctac tggaaagcgc tcatcaccgc cgggcgcgtc
    12361 aacaaagtga gggcgtaagg gtgcaaacga cttacaccca aatcagcgcg ggccgcagca
    12421 acgatgcctc ctggcttagc actaacgtgc acaaggtcat gcaggccgcg atgaagcagg
    12481 ctgaaattgc gctggatgat cttgcagcca ttcttccaca cggcaacggt tcggcagtga
    12541 gcgataacgc ggaagccaaa gccatcacca tgttcgcagg ggagcggcag atcctcgttc
    12601 tcgcctataa agggcagatt ggctataccg caaccggatc tggtgttgtc gatctgatca
    12661 ttggccacca ttcgttgacg catcatcaac tgatcgcacc tgttggtaac gacgtcatta
    12721 tcgacagcat ggcttcgctg gtacttacgg acggcagcgt gacaaaccat agcaaacgcc
    12781 atttgctgaa ggttggtgtg ggtgtcgatg gttcagttat tggcgttgtc atgtcaaata
    12841 cgcaagcggg gcgcgcgaaa tgacggaaaa gagtatctac ctgtcttcct gggcgcttac
    12901 ggaaccggac gacatttttt atttttatca gccgacctgg ctcaacgcct gggaagcaca
    12961 tatgtacgaa agaggattat atggcactcg tgctaatggc tgggggctga agcgctttgg
    13021 cgttgagaac gatatttatg cgccggaacg gatccgtaat ccacaatata tgccttacct
    13081 tgaggcgatg gagcaggtaa ccgattctgc attacaacaa atgccgacta aagagcgcat
    13141 gaagttaatg aaatcgcgca cggcatttat ttatgccgat tcctgggggg aatccggtcc
    13201 gtttgaaaaa atttccagtg cgctgcatat cgccactatt gatacgcttc ccaagaattt
    13261 acttaagaag ttttcgataa cggaaccgac ttgcaagatt cgcggcgaaa aacaggcatt
    13321 cgttcaggcg ctgcgtatgg cgcaggatta tctcgcctgg gatgtattcg ataacgtggt
    13381 aatttgtgct gcctatcggg caattccggt gctggtattc tctgaagaag atgtgggtaa
    13441 accgcctaag cgtttatttc cccgtcgtgc tgatgaggtg aatttatctg ttgaacgagt
    13501 gggttgtttt attctcagcc agcgtgagag cgctatcagg ctgcaatgcg gggaatatca
    13561 ttccggcact gcgccacagc atgctacgga tattgatgtc atggccttca gcgaacggcg
    13621 acaaatgccg tcccggcctg ctgctggcgt tatcgacctg gtagcccatt acggagaaag
    13681 cggttgtctg acgcccgcgc tgagtatgga ttatttaacc cgtcatattc agcccggtgg
    13741 aaaaatgcgt acggtcattg ccgataaaca gtttggttat cactactttg accttgaata
    13801 ccttgaagga taacgacagt cgtatgcaat ctactattaa atcgttgccc gctatttcgc
    13861 ttagcaacat ttacaagtgt tatggcgctg gggcaggaaa ggttgatgcg cttaaaagca
    13921 ttaatcttga tatcaataaa ggcgaattcc tcgcactgtg tgggccgtca ggcagtggta
    13981 aaagtactct gttgaatatt ctctcgggta ttgataaacc gaccagcgga acggtgatat
    14041 ttttaaataa attactgaac caactgccag aagagcagtt ggctgtgatt cgtggaaaac
    14101 atttgggttt tatttttcag ttttttaacc ttatgccggt attaaatgtt ttcgataacg
    14161 tttatttccc actggtgctt aatggtcagt tcagtaaaaa agaagccact gaacggacgc
    14221 tgcattatct cgatagtgtt ggattaagcg gttttggcga tcgtaaacct gggcaactat
    14281 cgggcggtca acaacagcgt gtcgccatcg ccagggcact ggcccacgaa ccgatggtgg
    14341 tcattgccga tgaaccgacg ggaaatttag acctggcaac cggcgaggct attttggacc
    14401 tgttgttgac gattaatcag cagaccggca ccacatttgt tatttccacg cactccagcg
    14461 aattaaaagc gcgggcacgg cgagtggttg aaattcagga tggagtattg attcatgact
    14521 cagatgcata aaggattttt gctcatcggc gtctttttgt acgcggcgtt tgtggctccg
    14581 aagatttacg cggcaacccc cgacgctgtt aaacaggttc tgcaacaggg gctcccggcg
    14641 cagcctgccg cgctggcgac gctgaccgag gcgttaaaga aagagtatga agccaggcac
    14701 aatgtggtgg cgctggtttt ttacgcctat ggcttactgc gtcaagcgga cggttatgcg
    14761 gcaacaaacg attttatcca tgcctcagaa tacgccaaaa gcggcttttt ctggctggat
    14821 gaagcggtgg atttgcagga gaaaaatcaa cgcgtacggt atttacgcgc ccgcgtagat
    14881 gcctatttac ccgcagactc agggcgttgc gtggtgacag tcaaagacac cgaacacatg
    14941 ctggctgacc cggcgatatg gacgacaacg atccgcgacc atattctggc gatgcgttat
    15001 cgcgcattac gtcactgcaa ggacaccacc cgcgcgaatg cgttgttggc gcaaattaaa
    15061 gggcaaaacg ccgcgcttgc gcagtcgctg acgcaggatt ttaatgttgt atcagaatgg
    15121 gatagtgaag agttaaccca ggtactgctg ccgctgatga aaggtgagta aatgtccggg
    15181 gaaattttag ctgttaatag cgttctgctg ctgatcctgt tttttgtgtt ttatcagctt
    15241 ctgttttatt cacgagatgt caaatttgca tttttgaatc tctttcgcca caaacgtcgc
    15301 tctttttcta ccataaccgc aattgtattg ggtggtgtgg cgatcttttt atatggcgga
    15361 tttattgatt actctttctg gatcctgaaa gagcagacga ttcgtaccaa tattggtcat
    15421 gtgcagattt acaatcagca ctattttgaa acgtcgaata aaaataagag cctgattgcc
    15481 gattatgcgg cgttaaagaa agctattttg tcggacccga cgctggcggc ggatatttcc
    15541 acgctctccg ggcaactgga gtttactggc gtaatttcac attacgagag cgaaacttcc
    15601 agctatttct ccgcgctggg ggtggagccg ttacccgctt taaagctggg ttcgttcgat
    15661 aagattatcg cggggagcga tctttctcgg gtaaaaggtg atgaaatcac cctgggcagc
    15721 ggtctggcga aaacgttgaa tgcgaaatac gacgactggc tggatgtgat ggtggtcaac
    15781 acggcgggcg ggcagggcgc gttgtcgcta aaactgcgtg gcatttttga gtcaggcatt
    15841 aaagattatg acgatgtcgc gatgaagatc ccgctggaca ccgcgcagcg catgatgggt
    15901 acggatggcg tcagcaaagt gctaattctg cttaaagaag atgacaccgc ggcgtttagc
    15961 acaaagctgc gtcagtttat cgaacgcaat catttgccgc tggtggtcaa agactggcgt
    16021 gatgtttcac tcttttatca acaggttgaa gggttgctat cggggatcta tttcttcatc
    16081 aagctgattg ttgcgctgat tgtcatcttc atgatcggca attcgatggc tatgaacatt
    16141 gttgagcgca cccgtgaaat aaccactctg cgtgccatag gcctgaagcc gcttcacgtc
    16201 acgcgcttgt ttctgacaga aggcattttt attggtgtga ttggcgccgt cggcagcctg
    16261 ctggtggggg gcgtgttagc ctggattatt aatctttatg gtattgcgat gccgccatcg
    16321 cctggacaaa ccatcggcta taccgccttt ataaaaacca gtaacccgga attgatttgg
    16381 gtgacggtgg tattacccat tttgacagca acaggcgcct ctgtgttacc ggctttacgt
    16441 gcttcccggc tgaatatttc tgacgcattt aaattctcat agggctaatt atgaatatgg
    16501 ctttttacgg caagtggttt gcttgcctgt ggctcgctac gtcgtgcgtt caggcggcga
    16561 gcacagataa taaggcgctg gagataattc gtcgcgctga cgaaattcgt tcgccgaata
    16621 aaccgtttcg ttacacgctg acggtaacgg aatataaagc gggcgcaacc cagccagaga
    16681 ataaacaggt cctcgatatt tccatgcgct ttatgaaacc gcagggtaat gaaaaagcgg
    16741 atgcacgctc gctggtgcgt tttatttatc cgccgcgaga taaaggaaaa atcatgcttt
    16801 ccgactggta cgatttatgg ttttacacgc cggaactgcg acgtcctatg cctatttcac
    16861 ggcaacaaag attaattggg caaatctcta acggcgatgt cattgtcact aactttgaat
    16921 atgcctatga ctcgaccctg gtgggggaag tcacgtgtgc tgaaaagcag tgttacaagc
    16981 tggcgctggt gcgtaaatcg gcggatatca cctggccgaa ggttatctat tacgttgaaa
    17041 aagacggtga taatcgcccg tggaaggcgg cctattattc gcaggatgac cagttaataa
    17101 aagaagtgtt gtatcaggat ttccagccgg tgttggggaa aacgcgaccc atgaaaataa
    17161 ccgttaccga tgttcgtcac ggcaataact attcagtgat ggagtacagc gatgttcgtc
    17221 tggaatcgct gccggagttt catttcacca aagagtatat tcagcgaggt gcgaaatgaa
    17281 acgtacgctg aagatctctt cgttgctgtg tgttgcgctg ccgttgacgg tacaggcgga
    17341 ttgcctgtca ggtgacgagg tggcacaaaa cagcgatgtg acgctttacg agcaggtgtc
    17401 ttatgtcaat aaacaggctt cggcctggca gattgcaggc aaaaatccct acactcgtca
    17461 caacggttat caggaggcgg ggattggcat caacagtggt tgttcgataa tcgacaatac
    17521 gctggacctt aagctcaatc tgtatggaat aaacgaatat gcgcttaaac ccgcagggaa
    17581 attcgaaacg gatgatagcc gcacacgtgc gttaattaac cgcctcagtt tggtctatag
    17641 cgcctcggac agcgttcaat ttgaagcagg caaatttgcc gcgccgtcag gaatgttctt
    17701 tttgcgttca ccttctgatt tgcaaacgca ttattacacc ggattccagt cgacacgttt
    17761 gcacgatccc aaaatgacat cggcgtatca ggcgtcatcg tggggcgcaa aaatgaactt
    17821 ggatacacgc gattatgcgt tttcggcgag cgtcataccg aagttagcca ccatcgacaa
    17881 acgctatgtg acgtctggca actggtctgc taatcagcag ggaaacagcg acgaagccta
    17941 tctgctgagc tacagcgatc atcgctttgg tgagcacaca ccgacagtca atgtgcggct
    18001 cggaccatcg ccgtcactcg cgctgtctga cagttatcac tacaccccgc agctgacgct
    18061 gaatgtcgac gcagcctacc accgctccca acaatggcgg catctttccc accatgagac
    18121 ggcgcaggtc gaggaatatc agtttccgga ttcgctctat gaaacgaaag atgaaagcgg
    18181 cgtggagctg gcgctgggtg ggcagtacac cagtgataat ttcagcgtgt ttggcgttga
    18241 gtattacttt cagagcgagg gttattcgcg cgctgaacag cgtcagcagc gcgagttaat
    18301 tgacttcctc aataccacaa ccggctatgc gccactggat caggcatttg attcttacaa
    18361 atacctgatg gcttcagaaa tcagcaatac cgccaatcag gggatgctgc agggtaagca
    18421 ctatctgaac gcctgggcca gtctgccgct ggcgggagag tcgacgctgc agccttcgct
    18481 ggtggtcaat ctcattgatg gcagcacgtt gctgggcctg cattattcca cgccgctcag
    18541 cgctatcagt aaccagcttg aagcctatgc cggtggctat agcgcgctgg gaagtcggta
    18601 ttctgaattt tcgctttttg gcgacacgtt aggcctttat ttgggcttta aatactatct
    18661 ataaggataa agagatgatg aataatgctt cacaaccgac agtactgctt tttgccggtc
    18721 agggtaatcc ggttattggc atgggcagcg atctttggga tctcaacgcc agtacaaaac
    18781 atatctggga ttgtgccagc gatatttccg ggctgaacct gcgtcgcctg tgtcagaagg
    18841 ggccgatgaa tcgccttatt caaacgacgg tacaacaact ggcggtgacc gcaatcaatg
    18901 taacgctcta taccctgtgc cgtgaacgtc ttgagaattt aacggttgtg gggtcctgcg
    18961 gacacagcgt tggtgaatac agtgcgctct acgctgccgg agctatttct cttgagacgc
    19021 tatttggcat gattcacttc agagccagcc tgatgcatga cgaaagccag agaaataaag
    19081 gcgcaatgct ggcggttaaa ggcatcaacc gcgaacaact gcagacgatg attacggaat
    19141 caggcattgc gctggatatt agctgtgata acacgccgcg ccagcaggtt attgggggaa
    19201 cgcaggctgc gttgaatgaa tttgccacgc tgctgatggc cgctggatac gaaccggtta
    19261 aactcggtgt gagcggtgcc tggcacacgc gtttgatgga agacggcgtt caggcaatgc
    19321 gcgattatct tgccggactg gatatcgctt cgccagagca tcaggttctg atgaacgtaa
    19381 cagcaaaaag cgaggtcgct ccgtcaatta tcaaagaaaa tctctcatta cacttaactc
    19441 atacggtgaa atggactgaa tctctcgata catttctgaa tatgccaacc tcggtagcct
    19501 tccttgaaat aagtaataag ccctatttgg ggaatatgtt aaacgatttt gctggtgtgg
    19561 atcagcaacg agttatgcat tgtcgtaaag catttactga tgcgaaggta ttcaaatgat
    19621 tcctgattat ttaactttta ttcgctttca ggataaacga agtctgatat acatttatgc
    19681 tattggactt attctgatag gcttttattg gaaaaatgca ggatttactt ttccatcaga
    19741 ggatattggt gtcgttagtg ggattctggc cctggtgctg tataatttta tttttgatct
    19801 caaggcgtac tgggcttata aatgcgtcac gaagaatatc gatttttcgt ggtttaagaa
    19861 aaagcagaac cacaaaatag aattatttct tacgcaacct ctggtggcag gatttctgtc
    19921 gttaatcatg ttgagtgcaa tgagttgggg gctataccag cgtctaccct cgttatatgc
    19981 gctgttcctg atttcgttac ttgggccgtt ggttatcttt ctgttgtttc ggatgatccg
    20041 caccagttat gtcaagcagg tcgctatttc agtggcgaaa aaagtaaaat ataaaagtct
    20101 gactcgctat gtgctgcttt cggtgtgcat ctcaacggtt gttaacttgc ttactatcag
    20161 cccgttgcgt aacagtgatt cttttgtgat agaggggcag tggttaacgt ttaaatcgat
    20221 aattgcattg ctcattcttt gtggcgtagt gttggcgatt aatctgtttt ttctgcgctt
    20281 ctccaggcgg tacgcttttt tgggcaggct ttttttgcag gaaatcgatc tctttttctc
    20341 cagtgaaaat gcgttgtcga ccttttttgc caagccgctt tggcttcggt tattcatatt
    20401 gctggttatt gaagtgatgt ggattacgct ggtgtcggta ttggcaacgc ttgtggaatg
    20461 gcggatttgg tttgaagcct attttttact ctgctatgta ccgtgcttaa tttactattt
    20521 tttctattgt cgattcctct ggcataacga ttttatgatg gcatgtgaca tgtatttccg
    20581 ttgggggcat tttaataagt gaggaagtga taggaagtga ccagataata catatatgtt
    20641 ctgtactctc ttgcgcattt tgattgttga ctgagcaacc agacagttga tgtgcacgat
    20701 ttcccctcgc cctaacagac gtgggcgggg gcacccggtg tgtgcaatat acgaaaaaaa
    20761 agcccgtact ttcgtacgag ctcttcttta aatatggcgg tgagggggac cgcctgaaat
    20821 aaatctgact taatccaggg gtgttatata atttcattat aaacaatgca ttacactttt
    20881 tgttgtccga tattatctaa tgtattctta gtgaagcgag agactaatgg catactggat
    20941 ggcataccag agtgacggga attagcgtat ggcagtattg acggatacga aagcaagaca
    21001 tatcaaacct gatgacaaac cattgcccca tgggggaatt accggactga cccttcatcc
    21061 ttcttcagta aaggggcggg ggaaatgggt ttttcgttat gtaagtccgg tgacacaaaa
    21121 aagacgtaat gcaggattgg gaacttaccc agaggtcagt attgctgaag ctgcacgtac
    21181 tgcccggata atgcgagagc aacttgctgc aggtgatgat cccctggaga ttaaaaaggc
    21241 tgaatctgag aaagtcgtta tcccaacatt tgccgatgca gccaggcgtg tacatgcaga
    21301 actgtctcct ggatgggaaa atccaaagca tgtaaggcag tggttatcga cgcttgagaa
    21361 ttacgcgttt cctcaactgg gagcaaaaac gctggattcg attacggctg cggacgtggc
    21421 agaaacactg cgtccagtct ggttaacctt gtcagaaacg gcaagccggg ttaaacagcg
    21481 cattcatgtt gttatgcagt ggggatgggc gcacggtttt tgtgtggcaa atcctgttga
    21541 tgtggttgac catttgcttc ctcagcagac aagaggacgt gatgaacacc aaccagcaat
    21601 gccctggagg cagttaccgc tttttgtggc gaccagtgtg tatagtgatg aaccttataa
    21661 tgttacccgc gcactgttat taatggtgat acttacagca actcgctcgg gcgaagccag
    21721 gggaatgcgc tgggctgaaa ttgattttca taagcgggta tggactatac ctgcagaaag
    21781 aatgaaagcc aggctacagc atcgtgttcc tttatcccgg caggctattt acattctgga
    21841 aaatatacgt ggcctgcatg atgaactggt gttcccttca cccagaaagc agcagatcct
    21901 ttccgatatg gtgttgacaa gttttctgcg taaaaagaaa gccgtcagtg acattccggg
    21961 gcgagttgcc acggcacatg gttttcgctc aacattcagg gactggtgta gtgaacaggg
    22021 gtattcgcgg gatctggcgg aaagggcgct cgctcatacg ctgaaaaata aggttgaggc
    22081 ggcatatcat cgtactgatc tactggagca gcgtgtaccg atgatgcagg catgggcgga
    22141 ttatgtgatg tctcaaattg tgaataaata atagtgaaaa tgtatattgt acgatcatga
    22201 tttgatatgt gatcgtggca gtagtaatta aggaaccgct tttatgtcaa atgtattgag
    22261 ctgataatta tcagatcgtc aggaaagcgt atttttataa acaggggatt actcaacaat
    22321 ctcctgttta taaaaaacaa tttgcctgtt ttaacgtttc actcctgctg agactgaatt
    22381 attcctagtg cgtcataaat cagtcttctg cgtagtttat tgtt
//
LOCUS       DAZGCQ010000082        22202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000082 DAZGCQ010000000
VERSION     DAZGCQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.082"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHK07_003480"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHK07_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO8103765.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHK07_003481"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHK07_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO8103766.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHK07_003482"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHK07_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO8103767.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHK07_003483"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHK07_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO8103768.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHK07_003484"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHK07_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
                     /protein_id="HFO8103769.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKVRFHGMHSVMPRHPCDEIK
                     EPLRKAIQKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHK07_003485"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHK07_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO8103770.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHK07_003486"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHK07_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8103771.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHK07_003487"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHK07_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO8103772.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVKQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHK07_003488"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHK07_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8103773.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVLVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHK07_003489"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHK07_003489"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8103774.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPIAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHK07_003490"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHK07_003490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8103775.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHK07_003491"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHK07_003491"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO8103776.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHK07_003492"
     CDS             complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHK07_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO8103777.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12480..12653
                     /gene="ortT"
                     /locus_tag="ACHK07_003493"
     CDS             12480..12653
                     /gene="ortT"
                     /locus_tag="ACHK07_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO8103778.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHK07_003494"
     CDS             12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHK07_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO8103779.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHK07_003495"
     CDS             complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHK07_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8103780.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHK07_003496"
     CDS             complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHK07_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO8103781.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14118..15155
                     /gene="curA"
                     /locus_tag="ACHK07_003497"
     CDS             14118..15155
                     /gene="curA"
                     /locus_tag="ACHK07_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO8103782.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVEESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKIFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHK07_003498"
     CDS             15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHK07_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO8103783.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHK07_003499"
     CDS             complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHK07_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO8103784.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18398..19459
                     /gene="yncE"
                     /locus_tag="ACHK07_003500"
     CDS             18398..19459
                     /gene="yncE"
                     /locus_tag="ACHK07_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO8103785.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISVI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHK07_003501"
     CDS             complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHK07_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO8103786.1"
                     /translation="MSKHNTDTSEQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21341..21958
                     /gene="yncG"
                     /locus_tag="ACHK07_003502"
     CDS             21341..21958
                     /gene="yncG"
                     /locus_tag="ACHK07_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO8103787.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            22034..>22202
                     /locus_tag="ACHK07_003503"
     CDS             22034..>22202
                     /locus_tag="ACHK07_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO8103788.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVKMFHLLSQAFYNLK
                     MFLMMDMLGVGD"
BASE COUNT         5643 a         5463 c         5701 g         5395 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtctcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgt cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttaggacaga
     1381 ttgagcgtaa tgagtccagc ccgacggtcg cgacattatg gaaaattgcc actgggttga
     1441 acgtgccatt ttcgacattt atttctccgc cacagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcatttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcacccggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggtgaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gtagaaaaag cgaagttcct ttctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggccacgg
     2341 acgcgaccat tgaattcttt attcatggcg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggcgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgctggat gccattattg aagaacgtgg cgatctgacg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtgggtgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgatccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgcccgc atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggg gtcagcatca
     3181 cgcatacgct ggatgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccatctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat cctcaaacac atctgagttt cctcgcgaac gtttacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgagg
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggtaaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgccgaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac ttattgaatc tgcatgatat tgcctgccgg gtaaggcgtt
     3901 acgccgcatc cggcatcaaa cgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca ttgcattctg ccgttgcggc aatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acactaatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa agtcaggttt catgggatgc acagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc agaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagagacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga tatccctgaa gcgttgaccc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc cgttaccttc gccattaaat agtcacgatc
     4801 attttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt accttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgactg
     5221 tcagccatgc ctatcagttg ctcgaaagtc agggatatat tatcgcacga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacggc gatgagtgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagt ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggagtatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaatcctc tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttgctc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcagctt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttacct gcctgcggaa gtgaaaattc atcataatga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattgagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaactcc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gctgaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcagcatga caataatgac cgctcacgcc gccgaacccc ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatatatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaaagca attcgaaaaa gagacaggct gcgcggtgaa tgtgaaaact
     6961 gcggcgactt ccgatgaaat ggtcagcctg atgaccaaag gaggttacga tctggtcacg
     7021 gcgtccggcg atgcctcgct gcgtttgatt atgggtaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaac
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctatattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctcaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtc
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca gcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccgggttcg ttggtacatc gaatgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcggccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgctggtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg ctggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aacgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgg aatccggggc
     9001 tggggctgtt tttgctgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctaac gctgttatgg cagggctttt atacctttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aacatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatagcgtg gtatatggcg cgctatacca gcgggaaaat gaaagcgttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 cagcgttcct tacattacct gcggtgggcg gcaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggctgc cgttcatgat cctgcccgtg caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcagctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ttgccgctgg cgatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggc ccgccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctta tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgttgttt tcctacattt tcccatcctg ataatcgccg cctatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga tgcagtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatcgcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgt tattaaccgc ctttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct catggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgt tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgacct ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgaccgc gtgatgtacc ggtaaccaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aaaatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcgccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccggca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gcgatccgct gaccggacat cctaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgc cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgccggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc tcttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taaggataat attgcagatc gtaagagtat
    12121 gagatgatct tacgtactgt cagagcactg atttctctga cagtacgtgc tgcgtgcaaa
    12181 atcactacat caaataaacc agccaaatct catagagata agagtaaaca aaccaacagc
    12241 agcaaagaca tttatcagta ctacagttct actggaaaca ttcatttttg ccacctttta
    12301 tcattgcccc tatgtgtata gtacgggtta agaaaatccg caataatact cattctgaag
    12361 atggataagg gcaagttgct gtttgatgat tttttctcaa actggtgaaa aaacttgatg
    12421 cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga cgcccgtaca
    12481 tgtctctcta tcaacacatg cttgtcttct atgcggttat ggcagcaatc gcatttctta
    12541 tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc tttctggtgg
    12601 gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt taaatcttac
    12661 tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat aaatttcaca
    12721 agtggctaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt ggatgccgcg
    12781 attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag cgatgacgcg
    12841 gagttaagtc gggaagatcg ctacacccag cagcagcgtt tgcgcaccgc tattgctcat
    12901 cacggtcgca agcataaaga agatatggaa gcgcgccatg aacagttaac caaaggcggc
    12961 accatcctgt gattaaaatg agcgtctggc taccaaccag gcgccaacca caagtaaaat
    13021 agcgccacaa attgggccga acaacgcccg tagtggtacg ccatgactgc ggaagatatc
    13081 cagaaccaga ccgccaatca gctggctggc gaccagtact gcaatagttg tcgccgctcc
    13141 cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca agccaggtat
    13201 aagtgtccac cagcgcacgc tggacaccag ttcgccaaac cccgccatac cttgtttaaa
    13261 ccataaaata gaaacaaaga gcacaatccc caccagtgag ttcagcaaca tggcgatgag
    13321 aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta ccagaccaat
    13381 tccggcggca atcaaaaagg caagggtgag cgactgattc atccaatcgc gtccggttca
    13441 gtgcgttcgt cgagttgcaa ctgcataaat gtcagatcca gccagcgccc aaatttagtg
    13501 cccacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtgcag atgcagcgag
    13561 gcctgatttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc tcgcgcttca
    13621 tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc gggatggaca
    13681 taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc gccaaatgag
    13741 gcatatcccg tcactacgcc atcttcttca cttaccagca ccggataacc tgctatggtc
    13801 cgcgcttcaa accaggcaat gcggttatct gcatccaccg tttggtcatt ccagatagcc
    13861 gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc ggctttgcgg
    13921 gcaaaacgga tggacatgtc tggcctcaat aaaataatga tgatgatgtt atcaatacta
    13981 tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg cagagggggt
    14041 gtgacggctg caaacaaaac caatcctgcg agcctgctac tattgatgga ttcatgccaa
    14101 aagcacagga gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct
    14161 cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat gatgtcgcca
    14221 caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctggac ccgtatatgc
    14281 gtggacgtat gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg
    14341 tcggcggtac ggtgagccgt gtcgaggagt cgaatcatcc tgattatcag tctggcgact
    14401 gggtgctggg ctacagtgga tggcaagact atgacatatc cagtggtgat gatctggtga
    14461 aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctaggg atgccaggct
    14521 ttaccgctta tatgggccta ctggatatcg gtcagcctaa agagggcgaa acgttggtgg
    14581 tagctgcggc gacaggacca gtgggggcga cggtggggca aatcggcaaa cttaaaggtt
    14641 gcagagtggt gggggtagcc ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag
    14701 gcttcgatgt ttgccttgat caccacgcgg acgattttgc agaacaactg gcgaaagcgt
    14761 gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taagatattt gatgcagtgc
    14821 taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc agctataacg
    14881 ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa
    14941 aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc atccatgagt
    15001 ttcagaagga gatggggcaa tgggtgaaag aggataaaat ccactaccgc gaagaaatta
    15061 ctgacggttt agagaatgcg ccacagacgt ttatcggcct gctgaagggt aaaaacttcg
    15121 gcaaagtggt gatccgcgtg gcgggtgatg attaaagttt caacggcggc gtaagccgcc
    15181 ataaaaatcc aggcaaaaag cttattttca gctttaatta actaaccaca tcattgttct
    15241 gctgctaata acaacaaagg tgagtggttt atgattattc actttaatac accaggtgaa
    15301 ttccttctgc catgcaggca gggttggaca gaaaacacgc gaaggaatta aaatgcctgg
    15361 aacggaaaaa atgaaacatg tcagtttgac tctgcaggtt gagaacgacc tgaaacatca
    15421 gcttagtatt ggcgcactaa aacctggcgc acgcctgatt actaaaaatc tggcggagca
    15481 attaggtatg agtattacac ctgtgcgtga agcattatta cgtctggttt cggtgaatgc
    15541 gctttctgtc gcaccagcgc aagcatttac agttccggaa gtggggaaac gtcaattaga
    15601 cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg ctgttgaaaa
    15661 cctcaccccg caagacctgg cggaactcca ggaattgctc gagaaattac agcaggcgca
    15721 agaaaagggc gacatggaac aaatcattaa tgtaaacagg ctatttcgct tagcgattta
    15781 tcatcgctca aatatgccca tcctgtgtga gatgattgag caactgtggg tcaggatggg
    15841 acctggttta cattatctct atgaagcgat taatccagcg gaattacggg agcatataga
    15901 aaactatcat ctgttactcg ctgcgttaaa agcaaaagac aaagagggat gcagacattg
    15961 tcttgctgaa attatgcaac aaaatattgc tattttatac cagcaataca atcgttaaag
    16021 taaatgcccg gcaaataccg ggcatcaagg taattactca aatcgccatg cgatattcac
    16081 gccgacacca taatttcgtc cgggcgcagg ttcgtaatat cgcccgtttg actcattgac
    16141 aatgacagaa ccaacgtatt ctttatcgaa taaattatcg acacgaccaa ataaatccac
    16201 agttaaattg tggtaattgt atttataccc ggtgaataag ccgatgagag tataagacgg
    16261 cgccttggcc gtattttcat catctgccat aatatcgccc atataacgcg cttccgtgcc
    16321 tgcataccag ccatcttccg gtacataacc tatcgacgca aagcccatat tacgggcgat
    16381 ccctggcatc cgattaccgt tacaatcctg ttcattgcaa acattgctgc gataggtcgc
    16441 atcaagccag gtccaggacg cgtttacgcg aaaatctccc gcgaaacgtt gatcccatgc
    16501 cagttcagcg ccttgacgac gggtctttcc agcatttttg taagtcgtac gcccaccgct
    16561 actgctatcg acaacaattt catcatcggt gtcggtctgg aacaatgcga gactaagcag
    16621 cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtgg atggttttaa
    16681 accaaagttc ataccgcttt gcccatcagc gcgataagac agctcattaa tcgtcggcgt
    16741 ttcaaaacca cgcccggctg ccagatagat attccaggca tcggtcattg catattttaa
    16801 cgatccggct ggtagccatt tgtgataact ggcatcaccg ctgtcatcgc cgttacccgg
    16861 agtaacgtaa tggtcgttgg aatcaaacca cacggagctg tagcgcacgc cagcatccag
    16921 cgacagtttt tcgctcagct gccactgcgt ctgtaaatag ggatcgatgt tccacatcag
    16981 attgcgttcg tcgcgacgca actcaccttt ttgcccgtac tccggcatgc cgctattcag
    17041 gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca ggccggtagt
    17101 gaacgtgacc ggaacgccca gttcaccacg gtgtgtccag cggctgtcta ttccctggta
    17161 atggcgttgc agggtaatca cgccgcccgc atgtgacggg ttaagttgtg gcgccatggg
    17221 tattgactgg tactgggtcg tttctcgctc tccggcatac atcatcacac tcatatcatc
    17281 ccgcgaactc aggctacgtt catagcgcaa cccagcctga gtttgcttga tggtttttcg
    17341 cgtgtcgtac tgttctgcac gaggcgcttg ttgtggattc gccttccatt ctgctttggt
    17401 tagcccaccc gggtcatctg ctttgatatc cacactattg aaaatcaggc ttaatttgct
    17461 ggcttcatca atgcgtacgc ccagtttggc attggctaaa tttttctgtg cgccactatg
    17521 gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcaa catcgccggg
    17581 ctgtgtgccg tctcccgttg cgcccgttgc tttcagccca tagcgccagc tgccaaaact
    17641 gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg tggtgacatt
    17701 cattacaccg ccagacgcgt tgccatacag ggcagagaag gggccacgca gcacttccac
    17761 attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg gcatggtggc
    17821 gggaataccg tccacataca ggcgaatacc gcgaattccg taagtggaac gggagccaaa
    17881 tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta cctgcaaacc
    17941 aggcacgccg gtcagtgatt cggacaagtt aatgcgcggt gttgccaggc gcatctcctc
    18001 gccatccacc acgcttactg ctgcaggggt atccagttct gaaaccacct gcggcgcggc
    18061 actgacaatc atggtctgtt catcagcggc aaaaacaacg ggggaaagga caagcagtgc
    18121 gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt taagaatggg
    18181 aaaacgccgt catggtaatg aaattgtaaa tttatggaaa atgaaacggc acaatacgtt
    18241 aagtaattga gaaaattgta gtcgtaacgg caagaaatgc tccacatttg agaaaataat
    18301 gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg aagcattgtg
    18361 aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg catttacgtc atctgttttc
    18421 atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat cattcagtac
    18481 gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg aaatggctta
    18541 tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac tggataaagg
    18601 tggcgtggtt tatcgtcttg atccggtcac tctggaagtg acgcaggcga tccataacga
    18661 tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt ttggtaacac
    18721 cgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag gccgtctggt
    18781 gcttgatgat cgtaagcgca cggaagaggt gcgcccgctg caaccgcgtg agctggtagc
    18841 tgatgatgcc acgaacaccg tttacatcag tgttattggt aaagatagcg tgatttgggt
    18901 cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta aaatgagtac
    18961 cggtctggcg ctggatagca aaggcaaacg tctttacacc actaacgctg acggcgaatt
    19021 gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc tggatgacgg
    19081 caaagagcac ttctttatca acattagcct tgataccgcc aggcagcgtg catttatcac
    19141 cgattctaaa gccgcagaag tgttagtggt cgatacccgt aatggcaata ttctggcgaa
    19201 ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg aagcctacgt
    19261 aacgcatcgt caggcaggta aagtcagtgt gattgacgcg aaaagctata aagtggtgaa
    19321 aacgttcgat acgccgactc atccaaacag cctggcgctg tctgccgatg gcaaaacgct
    19381 gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc cagacgatgt
    19441 gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact tgcctgactt
    19501 ttatcacgcc gcatgcggca caccttcgtc agtaatctaa atttgcccgg cacgtagccg
    19561 ggcaattttg caattagctc gccgtttcag gcttaaacac aatttcctgt ttttcttcat
    19621 cttcctcgac gactggcgca gtgctgtgaa tttcagcaac gcgtttgcgc acaccaaacc
    19681 agcctataac cagcagaata ccgataatcg gcagcgccgc gatagtataa gtcccgttcg
    19741 ggtaatcgaa cgccatcagc acaaggacac tcagtaaaaa cagtaatgtc agccaggaag
    19801 tgaagggcgc gccaggcagt ttaaaactga catccgctgc tttgccttct ttaatcgctt
    19861 tacgcaggcg catctggcac acgatgataa acgcccatga agcgatgatt cccagcgacg
    19921 cgaagttcaa cacaatctca aatacgcgcg acggcaccag atagttgagg aatacgccga
    19981 cgacatacac aactagtgtc gccagaatcc cggcatacgg cacatgctga cgactcattt
    20041 tcgccataaa actcggtgcg gaaccgccca tcgccattga gcgcagaata cgtccggtgc
    20101 agtacagacc tgaattcagg ctggagaggg cagcggtcag caccacaatg ttcataatgc
    20161 tgccgatata tggcacaccc agtttagaga aaaacgtcac gaacggactt tgccccgcct
    20221 gatacgcgct ccacggcaat aacataacca gcaacaccac ggagccgacg taaaacaggc
    20281 caatacgcca aatcacactg ttaatcgctt taggcaccat ggtctgcgga tctttacatt
    20341 ctccggcagc tgtacccacc atttcaatgg aggcaaaagc aaacactacg ccctgaatca
    20401 acaccagcgc aggcagcaaa ccgtggggga agaagccgcc attatcggtg attaaatgaa
    20461 agccagtggt gttgccatcc agcggctgac cactaccgag gaacactgta cccacgacca
    20521 aaaaggtcac aatggcgagc actttaataa gcgcaaacca gaactccatc tccgcaaacc
    20581 atttcacacc gatcatattc atggtgccaa cgatggtaag tgcagcgagc gcaaagaccc
    20641 actgcggcac gccgccaaac gcaccccagt aatgcatata cagagcgacg gcggtaatat
    20701 caacaatccc ggtcatcgcc cagttgatga agtacatcca accggcaaca taagcggctt
    20761 tctcacccag aaactcacgg gcataggaga caaagctgcc actggaaggg cgatgcaata
    20821 ccagctcacc caacgcgcgc agaataaaaa acgaaaacag gccgcagatt aaatagacca
    20881 gtgccagcgc cggccccgcc atttgcagtc gggctcccgc gcctaaaaac aaaccagtac
    20941 caatcgcacc gccaatggcg atcatctgca cctgacgatt gcccattgct ttgtgatatc
    21001 cctcttcgtg ggcattaagc cagcggcgtt tcgcagcgtg ttgctctgaa gtgtcggtgt
    21061 tgtgtttact cattgcgctc cctgattgct ttaatgaaaa aagtctttta tgtcgccatg
    21121 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
    21181 ctatcggcgc agcatcctac ccgttatcga taaacgatgc aaaacatccc cttacaatcc
    21241 tgaaggggat taatacaact gacgaaaaaa tgacaaatcc ttttgctggt taacctgtgt
    21301 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
    21361 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
    21421 gttcgttgat gtcagtggtt ttgaccacga gggagcttca cgcgagttat tgaaaaccct
    21481 gaacccgttg tgtcaggttc cgacactggc attggaaaat gacgagatta tgacggagac
    21541 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
    21601 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
    21661 attcactttc gccgattacc ctgagcgttg ggctcctgac gcacccgaac agttaaagaa
    21721 gaatgttatt gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga
    21781 accttatgcg tttggtgagc aactaacgct ggtggactgt tatctttgca ctatgcgcac
    21841 atgggggcct gggcatgaat ggtttcagga taatgccacg aatatcagtg cgattgctga
    21901 tgctgtatgt cagttaccaa cgttacaaga aatactaaaa aggaatgaaa ttatttgagt
    21961 gggataccag aagattatgt tatttattat tcagtaactt tatccacaat gatgggcgta
    22021 attaattaga ttcatgttat gttttttaat ttatatcact ctccctttca ttcagcttgt
    22081 ctatttcatt tcctctgaaa agaaactaac tattcacatt gtgaagatgt ttcacttgtt
    22141 atcacaggca ttctataatc tcaaaatgtt tttaatgatg gatatgctcg gagttggaga
    22201 ta
//
LOCUS       DAZGCQ010000083        22133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000083 DAZGCQ010000000
VERSION     DAZGCQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.083"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHK07_003504"
     CDS             complement(<1..150)
                     /locus_tag="ACHK07_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103789.1"
                     /translation="MPRTCILYAHPSTTAGCGVNALSGLRMAREFVGLIRRVSVASGI
                     CARLPD"
     gene            complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHK07_003505"
     CDS             complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHK07_003505"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO8103790.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHK07_003506"
     CDS             complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHK07_003506"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO8103791.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHK07_003507"
     CDS             complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHK07_003507"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO8103792.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHK07_003508"
     CDS             complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHK07_003508"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO8103793.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHK07_003509"
     CDS             complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHK07_003509"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO8103794.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHK07_003510"
     CDS             complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHK07_003510"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO8103795.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHK07_003511"
     CDS             6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHK07_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO8103796.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7566..8342
                     /locus_tag="ACHK07_003512"
     CDS             7566..8342
                     /locus_tag="ACHK07_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO8103797.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            8362..9315
                     /locus_tag="ACHK07_003513"
     CDS             8362..9315
                     /locus_tag="ACHK07_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO8103798.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            9352..10434
                     /gene="lacI"
                     /locus_tag="ACHK07_003514"
     CDS             9352..10434
                     /gene="lacI"
                     /locus_tag="ACHK07_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO8103799.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEYLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHK07_003515"
     CDS             10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHK07_003515"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO8103800.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFSAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLRGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGRGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLPDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            13683..14936
                     /gene="lacY"
                     /locus_tag="ACHK07_003516"
     CDS             13683..14936
                     /gene="lacY"
                     /locus_tag="ACHK07_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO8103801.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            15002..15613
                     /gene="lacA"
                     /locus_tag="ACHK07_003517"
     CDS             15002..15613
                     /gene="lacA"
                     /locus_tag="ACHK07_003517"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO8103802.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSDV
                     EKRESLIKEMFATVGENAWVEPPVYFSCGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHK07_003518"
     CDS             complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHK07_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO8103803.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTQVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHK07_003519"
     CDS             complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHK07_003519"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO8103804.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHK07_003520"
     CDS             complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHK07_003520"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO8103805.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            18173..19072
                     /gene="cynR"
                     /locus_tag="ACHK07_003521"
     CDS             18173..19072
                     /gene="cynR"
                     /locus_tag="ACHK07_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO8103806.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHTALEECADVGENESR"
     gene            complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHK07_003522"
     CDS             complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHK07_003522"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO8103807.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKICAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHK07_003523"
     CDS             complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHK07_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO8103808.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGLADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         5228 a         6119 c         5815 g         4971 t
ORIGIN      
        1 atccggcagt cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
       61 ttctcgcgcc atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg
      121 atgggcatac aaaatgcacg ttctcggcag tcaatattat ttgttgttgc gcagatccag
      181 cgccacgtcg acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
      241 gagaatatcc accgcgctta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
      301 gctggagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
      361 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggcccc
      421 atgctgccaa cccagtttat ccacggcggc aataaatact tccagcggcg cgttacctgc
      481 gcccgctccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
      541 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc
      601 tggtttcagc actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
      661 accgccagaa tccaccacat aaatgcaggt cgcaccgtag ccttccatca gctttgcctg
      721 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
      781 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
      841 agccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
      901 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
      961 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggacc cctgcaaacc
     1021 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     1081 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac
     1141 gtccgatata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     1201 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca
     1261 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     1321 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     1381 acgcgctgtt tcaggcgata tcccggtacg taagcctgca ccgcctcagc catttcattg
     1441 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     1501 atcatcaacg gtggctctgc tgggttaagc acgataattg ccttcccttt tgccgcgccg
     1561 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     1621 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     1681 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatattg
     1741 acgttcagtt gttcgacatt cgcatcaagg ttaaccaccg gcacgcagta agggccgatg
     1801 gcagcaggcg tcaggtcgat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg
     1861 tttttcacat gtgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     1921 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
     1981 cgcgccagac cgtcggactg aggatcaata ccgaccatca ccgccatctc cagatgctga
     2041 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
     2101 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
     2161 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
     2221 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
     2281 ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
     2341 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
     2401 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
     2461 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct acccaccacc tccagcgccg
     2521 gaagtaccca ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat
     2581 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
     2641 gaatgatttc gttatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat
     2701 caacgcccag ttgttgctgc actttcggat gtgtcaggcc cactttacgc cccaccacgc
     2761 gacgcccctg cgcaacgtca tattgcacat ttatgtgctg aatggcgtag gccgcttcag
     2821 cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
     2881 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagcac
     2941 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccaat
     3001 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
     3061 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
     3121 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
     3181 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
     3241 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga
     3301 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
     3361 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
     3421 agttaagggt gaacgccaca gaactatggc cacccatcga gttgcccagc aggtggattt
     3481 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgcg
     3541 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
     3601 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
     3661 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
     3721 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
     3781 tttgtggctg ataactcata atcttctcct gcatattcag ttctctgttc tggcgcttaa
     3841 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
     3901 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
     3961 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
     4021 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
     4081 ctgatcctca acaaacttct cagcggcgct aatcaccctt tgctgacgca attcgcgctc
     4141 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
     4201 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
     4261 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
     4321 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
     4381 cccaccgagc aggaactcca gcggctgtgc gaacccgtgg tccacctgca tacagtaaga
     4441 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
     4501 aggcactggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
     4561 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
     4621 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
     4681 gacctcatcg agtacctctt gtgctgggtc gacatacccc accagcggcg agtgggaaag
     4741 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
     4801 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
     4861 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
     4921 accagctacg caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct
     4981 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
     5041 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
     5101 cgaagttcgc gccgatcgcg ttatcgagta gcgtcacgtc gccgttttcc agcgtgactt
     5161 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
     5221 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
     5281 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
     5341 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
     5401 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
     5461 ccagtttcca tgcgaggtta aaagcgtcgc gcatgccact gttatagccc tgcccctgcc
     5521 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
     5581 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
     5641 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg
     5701 tttctcctgg catcaccata aattcaaagc gacgtaccgc atgaggtagc gcggcagaaa
     5761 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
     5821 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg
     5881 tgcgacggac aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgactattt
     5941 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg
     6001 cctccagctc gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca
     6061 tcaccgcatc gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
     6121 tcattggctg aatatcagca aaacagcggc ccttcggggt gagaaaacgc atcgcgtgcc
     6181 acggcgtagt atgcggcaga acattatcga ccaggccgac cgactgcatg gtgcgcagcg
     6241 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
     6301 ccagcacgtc aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg
     6361 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
     6421 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
     6481 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata gaaagggtgt
     6541 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
     6601 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
     6661 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgacggcggt gccagcgtcg
     6721 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
     6781 tgcaggaaga gggatatgtc cggcgtagtc cctccgatga tagttttcgt ctgaccatca
     6841 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
     6901 cactgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
     6961 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
     7021 caatggtcgg gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct
     7081 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg
     7141 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgt gcgcgcaaag
     7201 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
     7261 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
     7321 caatgaccat cgaacaggca gcggaaaagc atcttccggc actacaacgg gtagcaaaac
     7381 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
     7441 atttacgcta attacgccaa tgtgtaattt ggcccattct catcgttatt cgcccatttg
     7501 cccacatgca cagtacgggc tgcacgctac gctaatgaca tcaatactgt gatgtggagc
     7561 aaatcatgaa aattacgcaa attcgtaatg ccacccaaat tatccaatac gctggcaaaa
     7621 catttcttat cgacccgtta ctcgcgccta aaggaagtta tccgggtttt gccggaacgg
     7681 cacgcgcaga aatccgcaat ccaacggtag aattaccgtg cgatttaaca acgttactca
     7741 acgttgacgc cctaatcgta acccatttgc atgaagatca ctgggacgaa acagcctccc
     7801 gcgtcgtccc taaagataaa ctgatttatg tgcaaaatga gcaagatgct gaatcactga
     7861 ataaacaagg atttacgcat cttaccgtac ttaccgacga gactaaattt ggcgatatta
     7921 ctctgcataa aacgacaggg cagcacggct ccgatcgcac ctatgccgac ccggctatgg
     7981 cgaagcgttt aggcgcagcc tgcggcgttg ttttccagca cccgacagag aaaacgctct
     8041 atctggctgg cgataccctc tggcgtgacg acgtcgaggt tacaatgcta actttccaac
     8101 cagaagtggt tgtgttgaat gccggatttg cgcatgttct ggggcatggc gcgataatta
     8161 tgggagcaga agatattctc aaaacacatt ttaccctgcc agaagcgcaa attgtcgcga
     8221 cacatatgga ggccgtaaac cactgtttgc tgacgcgtga tgcgctcaag gaatacgcca
     8281 gagataatca aattatcgaa tttgtgaacg taccagaaga tggtgaaacg ttgacattct
     8341 aattcggaga taaagagtgt tatgcgcccg ctaactgttg ccattatcgc cgttgccggt
     8401 tttagtcctt ttcacctttc cgtaccgttt atcgtattta gcgaaaaaat ggcggagaaa
     8461 aaacactttc acgtaattat ctgcgctgaa aagccgggaa acgtggactc tgccgatggg
     8521 ttttccgtaa ccgccaccca tgactatacc gcagtcatcc aggcggatat tgtgataatt
     8581 ccttactggg gaaccattac acaaaaaccg ccacaaaaac tgctggaagc cttaacgacc
     8641 gcacgggata acggcgcaca aattgtcggg ctttgcctgg gcacgtttgt gctcggctat
     8701 gcaggtttac tgaaaaataa gcgtgccgcc acgcactggg agttcgagcg tgaatttcag
     8761 gcacgttttc cacaaacaca tctggatatt aacgcgttgt acgtagacga tgacggcatt
     8821 attacctctg ccggtactgc cgcggcgctg gattgctgtt tgtatattgt tcggcaacat
     8881 tttggcagcg actatgctaa ccatattgcc cgacggatgg tcgtaccgcc atatcgcacc
     8941 ggcggtcagg cgcagtttat tgagcaacca gtgccgaaaa atacccatga tgaacgcatt
     9001 aatctcctgc tggattacct gcggcaaaac attgcgcaac agcatgatct cgactcgctg
     9061 gcgcagcgag taatgatgag tcgccgcaca ttaactcgcc attttatgaa agcgaccggt
     9121 tcgagtatcg ccgaatggct cattactgaa cgcttacgcc gtagccagga actgttggga
     9181 tccagtcagt tgcccgttga gcggatagcg gctgaggtgg gttttctctc acctgtgacc
     9241 tggcgtcagc attttaaatc tcacttcggc gtcagccccg ccgaatggcg caaaaccttt
     9301 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     9361 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     9421 gtgaatcagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     9481 gagctgaatt acattcccaa ccgcgtggca caacaactgg cggggaaaca gtcgttgctg
     9541 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     9601 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     9661 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     9721 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt
     9781 ccggcgttat ttcttgatgt ctctgaccag actcccatca acagtattat tttctcccat
     9841 gaagacggta cgcgactggg cgtggagtat ctggtcgcat tgggtcacca gcaaatcgcg
     9901 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa
     9961 tatctcactc gcaatcaaat tcagccgata gcggaacggg agggcgactg gagtgccatg
    10021 tccggttttc aacaaaccat gcaaatgcta aatgagggca tcgttcccac tgcgatgctg
    10081 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggttgcgc
    10141 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc gtgttatatc
    10201 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
    10261 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
    10321 gtgaaaagaa aaaccaccct ggcgcccaag acgcaaaccg cttctccccg cgcgttggcc
    10381 gattcattaa tgcagctggc acgacaagtt tcccgactgg aaagcgggca gtgagcgcaa
    10441 cgcaattaat gtgagtcagc tcactcatta ggcaccccag gctttacact ctatgcgtcc
    10501 ggctcgtatg ttgtgtgaaa ttgtgagcgg ataacaattt cacacaggat acagctatga
    10561 ctatgattac ggattcactg gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg
    10621 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10681 aggcccgcac caatcgccct tcccaacagt tgcgcagcct gaatggtgag tggcaatttg
    10741 tctggttttc ggcaccagaa gcggttccgg aaagctggct ggagtgcgat cttcctgtcg
    10801 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgacgcg cccatctaca
    10861 ccaacgtgac ctatcccatt acggtcaatc cgccatttgt tcccacggag aatccgacgg
    10921 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
    10981 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
    11041 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
    11101 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
    11161 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcacaaa ccgaccacac
    11221 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gcggttctgg
    11281 aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt
    11341 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
    11401 agcgtggcgg ttatgccgat cgcgtcacac taggtctgaa cgtcgaaaac ccgaaactgt
    11461 ggagcgccga aatcccgaat atctatcgtg cggtggttga actgcacacc gccgacggca
    11521 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtacggatt gaaaatggcc
    11581 tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc
    11641 ctctgcgtgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
    11701 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacaccc
    11761 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
    11821 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc cgcgatgagc gaacgcgtaa
    11881 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
    11941 agtcaggcca cggcgctaat cacgacgcac tctatcgctg gattaaatct gtcgatccat
    12001 cccgcccggt gcagtatgaa ggcggcggag ccgacacctc cgcaaccgat attatttgcc
    12061 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
    12121 aaaaatggct ttcgttgcct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
    12181 cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt cagtaccccc
    12241 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
    12301 atggcaatcc gtggtcggct tacggcggtg attttggcga tacgccgaat gatcgccagt
    12361 tctgcatgaa cggtctggtc tttgccgacc gcacgccgca tccggcgctg acggaagcaa
    12421 aacaccagca gcagtttttc cagttccgtt tatccgggcg aaccatcgaa gtgaccagcg
    12481 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggca
    12541 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa caggtaattg
    12601 aactgcctga actaccgcag ccggagagca ccgggcaact ctggctaacg gtacacgtag
    12661 tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg cagcagtggc
    12721 gtctggcgga aaacctcagc gtgacactcc cctccgcgcc ccacgccatc ccgcatctga
    12781 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
    12841 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
    12901 gcgatcagtt cacccgcgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
    12961 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcgg
    13021 cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg accgctcacg
    13081 cgtggcagca tcagggaaaa accttattta tcagccggaa aacctaccgg attgatggta
    13141 gaggtcaaat ggcgattacc gttgatgttg aagtagcgag cgatacaccg catccggcac
    13201 ggattggcct gacctgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
    13261 ggccgcaaga aaactatccc gaccgcctta ctgctgcctg ttttgaccgc tgggatctgc
    13321 cattgccaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
    13381 cacgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
    13441 gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
    13501 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
    13561 cgtcagtgtc ggcggaattc cagcttagcg ccggtcgcta ccattaccag ttggtctggt
    13621 gccaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
    13681 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
    13741 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
    13801 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
    13861 aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta
    13921 ttaccggcat gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac
    13981 aatacaacat tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg
    14041 ccggtgcgcc cgcagtagag gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat
    14101 ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca
    14161 tcatgttcac catcaataat cagtttgttt tctggctggg ttctggctgt gcactcatcc
    14221 tcgccgtttt actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg
    14281 cggtaggtgc caaccattcg gcatttagcc ttaagctggc gctggaactg ttcagacagc
    14341 caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg
    14401 accaacagtt tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg
    14461 tatttggcta cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc
    14521 cactgatcat taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt
    14581 ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtagtt attctgaaaa
    14641 cgctgcatat gtttgaagta ccgttcctgc tggtaggctg ctttaaatat attaccagcc
    14701 agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac
    14761 tggcgatgat ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg
    14821 gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc
    14881 ttagcggccc cggcccgctt tccctgctgc gtcgtcaggt aaatgaagtc gcttaagcaa
    14941 tcaatgtcgg atgcggcgcg agcgccttat ccgaccaaca tatcaggacg gaatgagcga
    15001 aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg atatgtgcga
    15061 aggcttacct gaaaaaagac ttcgtgggaa aacattaatg tatgagttta atcactcgca
    15121 tccatccgac gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca cggtagggga
    15181 aaacgcctgg gtagagcccc ccgtctattt ctcttgcggt tccaacatcc atataggccg
    15241 caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa caatcggtga
    15301 taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg tacaccatga
    15361 attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata acgtctggat
    15421 cggaagtcat gtggtcatta atccaggcgt caccatcggg gataattctg ttattggcgc
    15481 gggtagtgtc gtcataaaag acattccacc aaacgtcgtg gctgctggcg ttccttgccg
    15541 ggttattcgc gaaataaacg accgggataa gcaatattat ttcagagatt acaaagttga
    15601 atcgtcagtt taaattagca aaattgcctg atgcgcttcg cttatcaggc ctacaaattc
    15661 agtgttctac gttagccgca tccggcatga acaaagcgca ggaacaagcg tcgcatcatg
    15721 cctctttgac ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca
    15781 gcccaacgac gcacagcgca tgaaatgccc agtccatcag gtaattgccg ctgatactac
    15841 gcagcacacc agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa
    15901 acgccaccag cttgccagca atagccggtt gcgcagagtg atcgagcgcc agcagcaaac
    15961 agagcggaaa cgcgccgccc agacctaacc cacacaccat cgcccacaat accggcaatt
    16021 gcagcggcag ccagataaag ccgcagaaac ccaccagttg taacgccagt gccaacagca
    16081 aaagtttgcg ccgatcctga tggcgagcca tagcaggcat tagcaaagct cctgcggctt
    16141 gcccaagcgt catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa
    16201 tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccgaagt
    16261 aaacacccag cgtccacgcg cgaggagtga aaaccacgcg aacctgagtg gttgttgtct
    16321 tgtgggaaga ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg
    16381 caggcagcgc ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg
    16441 gcgtcagggc ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca
    16501 ccagtggcgt gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga
    16561 tgccgatccc caccccacca agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa
    16621 gctcacgcat caatgcaccg acggcaatca gtaacaaact gatggcgacg ctgcgacgtt
    16681 cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtgaccaccg
    16741 gcagagcggt caacagggca gccacgctaa agctcattcc gcttgcctgg cgcaattgcg
    16801 gtagcagtgg cccgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa
    16861 ccagcagcat cgtgaacgtc ttttgatggt tacgcatggc tgtcagaacg gtttggtcgg
    16921 cagatattta ccatctaagg tgatgaccgc acgttcgcca ccttccgggt ccgccacttt
    16981 cttaacgtcg agtttgaagt taatcgcgct gataatgcca tcgccaaact tctcatgaac
    17041 caacgctttc agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg
    17101 gtcggttgga atacggtcat caatgcagcc acgcagtggg atcatctgta acagtagaat
    17161 ggcgtcttcg tcgagatcca gcttcgcccc gacctggcgg gcggcgtcgg caggaagcgc
    17221 ctgctgaccc agcaaagccg cggtcacaaa ggcttctgcc agcccggtgc cgtcggcaat
    17281 ctcggcaaat gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag
    17341 acgaatattg cgattaattt gcgactgaat catggtgcat ctcctgatgg tttaaaaata
    17401 aggttacgct gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag
    17461 tggcacaaac tggcgggttg cgccgtcaaa cgctgcgatg ctgccgcttt caatgtcgta
    17521 gacccagccg tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt
    17581 ttgcaaatta gccaactgag caatgacgtt ttcacgtacc atcgccgcag cttttgacgg
    17641 taaatcggaa tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca
    17701 gtgggagacg gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc
    17761 acagttggaa tgaccgcaaa tcacaatgtc agatacccga agcgcagcga cggcatactc
    17821 caccgaagca gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt
    17881 gcgaataacg aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct
    17941 gtcggagcag gagataaaaa gtgtgcgtgg gctttgctgt gtcgccaact gtttaaataa
    18001 ggcttcccgc ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt
    18061 caccgttaac ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag
    18121 gtaaaagtct catttatgat gagttctatt ggatttactt ataggttggc gaatgctctc
    18181 tcgacatatc aattattttc ttgccgtggc tgaacatggc agcttcaccc gtgccgccag
    18241 tgcgttgcac gtctctcagc ctgcgctttc ccagcagatt cgccagttag aggagagttt
    18301 aggcgtgccg ctgtttgacc gtagcgggcg aacgattcgt ctcactgatg caggagaagt
    18361 ctggcgacag tacgccagcc gggcgttaca ggaactggga gcgggtaaac gggcgattca
    18421 tgatgttgcc gatctgacga gaggatcgct gcgtatcgcc gtcaccccca cctttacgag
    18481 ctactttatc ggccccttaa tggcggattt ctatgcgcgc taccccggca tcacgctcca
    18541 gctacaggaa atgtcgcagg agaaaatcga ggatatgctt tgccgcgacg agttggacgt
    18601 tgggattgcc ttcgcgcctg tgcattcgcc ggagctggag gcaattcctt tactgacaga
    18661 aagtttagcg ttagtcgtgg cgcaacatca tccgctggcc gtccatgaac aggtggcgtt
    18721 gagtcgcttg catgatgaaa aactggtcct gctcagcgcg gagtttgcca ccagagagca
    18781 aattgaccac tactgcgaga aagcggggct gcatccacag gtggtcattg aggcgaactc
    18841 aattagcgcg gttctggagc tgattcgccg cacttccctt tccacattgt taccagcagc
    18901 gattgccaca caacatgacg ggcttaaagc tatttctctt gccccgccac tactggagag
    18961 aacggcggtt ttgttgcggc ggaaaaatag ctggcagaca gccgccgcga aggcattttt
    19021 gcacacggcg ttggaggaat gtgcggatgt tggcgagaat gaatcacggt agccactggc
    19081 acaggtctgc ggctggaatt gcctgatgcg ctacgcttat caggcctacg agttcagtgt
    19141 aaggcgttca cgccgcatcc atcataaact acagctggca gacagccgcc gcgaaatcat
    19201 ttttgcacat ggcattggaa gaatgcgcgg atgttggcgg gaatgaatca cggtagccgc
    19261 tgacacagat cagcggctgg aattgcctga tgcgctacgc ttatcaggcc tacgagttct
    19321 acgagttcag tgctctacgt aggccggata aggcgttcac gccgcatccg gcataaacta
    19381 cgctcgcttt gtcaacacac agcccagccg atcaccgttt gtaatcgatg gcttctggct
    19441 gctccagata tacggtggtt tgtgccggtt gtgtgctggc aatcaccttg ccgccacgta
    19501 ccgaataacg taccggaacc tgacggcgca gcgcatcaaa tccattttca gccggcagga
    19561 taatcaggtt ggcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc aacgtcctgg
    19621 cgctgtggtg ggtgattaaa ttcaggccat cgttaatctg cccatagccc atcagctggc
    19681 aaacatgcag ccccatatgc agcacttgca gcatattcgc cgttcccagc ggataccacg
    19741 gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc ggactccagc atctctttaa
    19801 cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg tccttgcaga tgaatattga
    19861 ccagcgggtt ggcgacaaag ttaataccgg acattttcag caagcggaac aggcgtgagg
    19921 tatacgcccc gttataggag tgcattgccg tggtgtggct ggcggtgact cgcgcgccca
    19981 tgccttcacg gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc tcgtcatcga
    20041 tctcatcaca gtgaacgtcg atgagacggt cgtatttttg cgccaaggcg aaggttttat
    20101 gcagcgactc cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc cccactacat
    20161 ctgcccctaa gcgtaacgcc tcttccagca acgcttcacc gttgggatac gacaaaatcc
    20221 cttcctgagg gaaggcgacg atttgcagat caatccacgg cgcgacttcc tgcttcactt
    20281 ccagcattgc tttcagcgca gttagcgttg catccgaaac atcgacatgg gtacgcacat
    20341 gctgaatgcc gttggcaatc tgccatttca gcgtttgcca tgcgcgttgt ttcacatcgt
    20401 catgggttaa taacgctttg cgctcggccc agcgttcaat gccttcaaac agcgtgccgg
    20461 actgattcca gttcggttgt ccggcggttt gcgtggtgtc caggtgaata tgtggctcca
    20521 caaacggcgg tataactaaa ccttgttcgg catccaggct gttttcagtt atgggcatca
    20581 cgccggattg cgcatcaatg gcgcagattt ttccgtcctg cagatgaatc tgccacagcc
    20641 cctctttgcc tggtaaccgg gcgttaataa ttgtttgtaa agcgttattc gacactgtta
    20701 gcctccacat gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt aaggatcaga
    20761 tagctcagcg cgccacctaa taccgcgttg accggaacaa ttcccggtaa ccagtggcct
    20821 gcagcaatcc ccagggcgac cgccagaatc gccacccaat tgacactcat catacgcgtg
    20881 gtcgcaaagt gctcatagcg gcgacggttc atcagatagt cggcaatgat cacgccaccc
    20941 actggaggaa tagctgccga aaggaaggtc aaccagccga caaaattgtt atacagccat
    21001 aatgcacaga ccgtaccgat aataccgttg attaccgaaa gggttttgct cgacatcccg
    21061 gtaatgttgg cgaaacctaa acccgacgca tagagcgcgt tatcgttggt ggtccagata
    21121 tttagcccca gcaccacaat cgcaggcaac agcaggccct gagcaatcat cacatcagag
    21181 atatccgcca accccagtgc cgccgctccc gctgcaccga aaataaacat caacgagttg
    21241 ccgaggaaaa aggccaccat cgccaccagc accgccagtt tggcattgcg accaaagcgg
    21301 acaaagtcag cggtgagcgt acccgcgctg ataaatgacc ccacaaccag cgccagcgcg
    21361 acattgaaat ctaatggttg tgcgggaacg accgctttta aggcgtccag gccgcccatg
    21421 ccgttaacgg ccagccacac ggaataaccg ccaagacagg cgatcgccgg aaccgcaatc
    21481 accgaaagaa ctgtcagcgc cgaaatgcca aagaagacgg tgacggtcat cagtaaaccg
    21541 gaaacggcaa tcagcaaatt aatatccagc ccggttgcct tacccaccgg aatggcaaac
    21601 atcgccacgc caacgccaaa ccagccaacc tgggtgccgc ccagcagcag tgaaggcagc
    21661 catgagcctt taacaccaaa cgagaagcga gcaagaagat gagtggtcag gccggttttt
    21721 gcgccaatgt aaccgagaaa tgaagtgtaa ataccgagga gaagattgcc gatgagaact
    21781 gcgaggaaga aatcatgata gctaagaccg gttccgagag tgccgccagt ccacatactg
    21841 gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg ccaatacccc tttccgcgcc
    21901 gactgcggga ctggcccctg gctaaagttg ttatcttgcg acacgaaatt cccccttttt
    21961 gctgtttgaa acccaaaaaa tccgccgcat tctattcatc tgaagataaa aagcaatcgt
    22021 tttcgtgggg aaatatattt tttatatgaa tgagtgcaaa aaggtggggg aaaaattatc
    22081 ggtgtaaaaa cagtgggtag gcctgataag acgcgtcagc gtcgcatcag gca
//
LOCUS       DAZGCQ010000084        22124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000084 DAZGCQ010000000
VERSION     DAZGCQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.084"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..508
                     /locus_tag="ACHK07_003524"
     CDS             173..508
                     /locus_tag="ACHK07_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO8103809.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            966..1409
                     /gene="yjdN"
                     /locus_tag="ACHK07_003525"
     CDS             966..1409
                     /gene="yjdN"
                     /locus_tag="ACHK07_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO8103810.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1542..2330
                     /gene="phnC"
                     /locus_tag="ACHK07_003526"
     CDS             1542..2330
                     /gene="phnC"
                     /locus_tag="ACHK07_003526"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO8103811.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2355..3371
                     /gene="phnD"
                     /locus_tag="ACHK07_003527"
     CDS             2355..3371
                     /gene="phnD"
                     /locus_tag="ACHK07_003527"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8103812.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLSDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISANDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3477..4256
                     /gene="phnE"
                     /locus_tag="ACHK07_003528"
     CDS             3477..4256
                     /gene="phnE"
                     /locus_tag="ACHK07_003528"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO8103813.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4277..5002
                     /gene="phnF"
                     /locus_tag="ACHK07_003529"
     CDS             4277..5002
                     /gene="phnF"
                     /locus_tag="ACHK07_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO8103814.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            5003..5455
                     /gene="phnG"
                     /locus_tag="ACHK07_003530"
     CDS             5003..5455
                     /gene="phnG"
                     /locus_tag="ACHK07_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO8103815.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5452..6036
                     /gene="phnH"
                     /locus_tag="ACHK07_003531"
     CDS             5452..6036
                     /gene="phnH"
                     /locus_tag="ACHK07_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO8103816.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            6036..7100
                     /gene="phnI"
                     /locus_tag="ACHK07_003532"
     CDS             6036..7100
                     /gene="phnI"
                     /locus_tag="ACHK07_003532"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO8103817.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7093..7938
                     /gene="phnJ"
                     /locus_tag="ACHK07_003533"
     CDS             7093..7938
                     /gene="phnJ"
                     /locus_tag="ACHK07_003533"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO8103818.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7935..8693
                     /gene="phnK"
                     /locus_tag="ACHK07_003534"
     CDS             7935..8693
                     /gene="phnK"
                     /locus_tag="ACHK07_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO8103819.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8804..9484
                     /gene="phnL"
                     /locus_tag="ACHK07_003535"
     CDS             8804..9484
                     /gene="phnL"
                     /locus_tag="ACHK07_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO8103820.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9481..10617
                     /gene="phnM"
                     /locus_tag="ACHK07_003536"
     CDS             9481..10617
                     /gene="phnM"
                     /locus_tag="ACHK07_003536"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO8103821.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10617..11174
                     /gene="phnN"
                     /locus_tag="ACHK07_003537"
     CDS             10617..11174
                     /gene="phnN"
                     /locus_tag="ACHK07_003537"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO8103822.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11161..11595
                     /gene="phnO"
                     /locus_tag="ACHK07_003538"
     CDS             11161..11595
                     /gene="phnO"
                     /locus_tag="ACHK07_003538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO8103823.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11597..12355
                     /gene="phnP"
                     /locus_tag="ACHK07_003539"
     CDS             11597..12355
                     /gene="phnP"
                     /locus_tag="ACHK07_003539"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO8103824.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12432..12761)
                     /gene="yjdP"
                     /locus_tag="ACHK07_003540"
     CDS             complement(12432..12761)
                     /gene="yjdP"
                     /locus_tag="ACHK07_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO8103825.1"
                     /translation="MKRHSTLFFFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12830..15091)
                     /locus_tag="ACHK07_003541"
     CDS             complement(12830..15091)
                     /locus_tag="ACHK07_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO8103826.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDMPLVNTL
                     LNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAYGEADVT
                     FPALQRRDELGELARAFNVFARNTESLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SLAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQEN"
     gene            15205..16725
                     /locus_tag="ACHK07_003542"
     CDS             15205..16725
                     /locus_tag="ACHK07_003542"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8103827.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHLLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLAIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16722..17696
                     /locus_tag="ACHK07_003543"
     CDS             16722..17696
                     /locus_tag="ACHK07_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO8103828.1"
                     /translation="MTTSEKTSAKKILMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            17715..18656
                     /locus_tag="ACHK07_003544"
     CDS             17715..18656
                     /locus_tag="ACHK07_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8103829.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18711..19394
                     /locus_tag="ACHK07_003545"
     CDS             18711..19394
                     /locus_tag="ACHK07_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO8103830.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19409..20269
                     /locus_tag="ACHK07_003546"
     CDS             19409..20269
                     /locus_tag="ACHK07_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO8103831.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFCSAGQA"
     gene            20266..21267
                     /locus_tag="ACHK07_003547"
     CDS             20266..21267
                     /locus_tag="ACHK07_003547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8103832.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQWTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21264..21971)
                     /locus_tag="ACHK07_003548"
     CDS             complement(21264..21971)
                     /locus_tag="ACHK07_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO8103833.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4823 a         6516 c         6272 g         4513 t
ORIGIN      
        1 tggaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
       61 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
      121 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
      181 accacactgc ccaaaatgca actccgaata cacctacgaa gataacggca tgtacatctg
      241 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
      301 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
      361 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtaa aaaacatccg
      421 cctggttgaa ggcgatcata acatcgattg caaaatcgac ggttttggtc cgatgaaact
      481 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
      541 tcgcgccgca tccggcaacg gtgccagatg cctgatgcga cgcttgcgcg tcttatcagg
      601 cctacaaatt cccgcaccat ccgtaggccg gataaggcgt ttacgccgca tccggcaaca
      661 gtgccagatg cctgatgcga cgcttgcgcg tcttatcagg cctacaaatt cccgcaccat
      721 ccgtaggccg gataaggcgt ttacgccgca tccggcaacg gtgccgactg cctgatgcga
      781 cgcttgcgcg tcttatcagg cctacaaatt cccgcaccct ccgtaggccg gataaggcgt
      841 ttacgccgca tccggcaaca gtgccgactg cctgatgcga cgctcgcgcg tcttatcagg
      901 ccgcctctca tctgtataaa tttcgaacta cacttaactg gcttctcttc actgaggtca
      961 ccatcatgcc gttaagtccc tacatctctt ttgccggtaa ctgttccgac gcgattgcct
     1021 attatcaacg cacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccaa
     1081 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
     1141 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gccatgccgt
     1201 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
     1261 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
     1321 ctttctgggc gcatggcttc ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
     1381 atgtcgtcaa acaacaacca acgcaataat ccgccgggag gcccgccctc ccgcactgtc
     1441 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
     1501 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgcg
     1561 tcgaacaact gagcaaaacc ttcaaccagc atcaggcgct gcacgcggtt gatctgaaca
     1621 ttcatcacgg tgaaatggtg gctctgcttg ggccgtccgg ttccggcaaa tccacccttt
     1681 tacgtcactt aagcggcctg attgtcggcg ataaaaagcc agaatgccgc gtcgaactgc
     1741 tgggccgcac tgtccagcgc gaaggccgcc tggcccgtga tatccgcaaa agccgcgcca
     1801 acaccggcta catcttccaa caattcaacc tggtgaatcg cctgagcgta ctggagaacg
     1861 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
     1921 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
     1981 atcagcgcgt ttccaccctc tccggtggcc agcagcagcg tgtggcgatt gcccgcgcgc
     2041 tgatgcagca ggcgaaggtg attctggccg atgaacccat cgcctcgctg gacccggaat
     2101 cggcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggt atcaccgtgg
     2161 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
     2221 gtcagggaca cgtcttctac gacggcagca gccaacagtt tgataacgaa cgttttgacc
     2281 atctctaccg cagcattaac cgcgtcgaag agaacgcgaa agctgcctga catccccatc
     2341 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
     2401 gcctcagcac cctgttaacc ccggcgcatg ccgaagagca ggaaaaggcg ctgaatttcg
     2461 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
     2521 atatggagaa gaagctgggc gtgaaggtca acgccttctt tgccccggac tatgcgggca
     2581 ttatccaggg aatgcgtttc aacaaagtgg atatcgcctg gtacggcaac ctgtcggcga
     2641 tggaagcagt ggatcgcgcc aacggccagg tcttcgctca gaccgtcgcg gcggatggat
     2701 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgagcg
     2761 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
     2821 gcttcctcgt ccccggttac tacgtctttg ccaaaaacaa tatctccgct aacgacttta
     2881 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaggtgg
     2941 atgttgccac taacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
     3001 tcaaagagct gaaagtgatc tggaagtcgc cgctgatccc aggcgatccg atcgtctggc
     3061 gcaagaatct gtccgaaacc accaaagaca agatctacga cttctttatg aattacggca
     3121 agacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcctcca
     3181 gcgacctgca actggtgccg attcgccagc tcgcgctgtt taaagagatg cagggcgtga
     3241 aaagcaataa aggactgaat gagcaggaca agctggcaaa aaccaccgag attcaggcgc
     3301 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
     3361 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgcagg
     3421 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
     3481 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
     3541 ttctcgccgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgcttatca
     3601 aagacggcgg caacatggca accttcgccg ccgacttctt cccgcccgat ttcagccagt
     3661 ggcaggatta cctcaccgaa atggccgtca cgctgcaaat cgccgtatgg ggcaccgcgc
     3721 tggcagtggt tctgtccatc ccgttcggtc tgatgagcgc cgaaaacctg gtgccgtggt
     3781 gggtttacca gcccgtgcgc cgcctgatgg acgcctgccg cgccattaac gaaatggtct
     3841 tcgccatgct gttcgtggtc gccgtcggtc tcggaccgtt cgctggcgtg ctggcgctat
     3901 ttattcacac caccggcgtg ctctccaagc tgctttccga agcggtggaa gcgattgaac
     3961 ctggcccggt ggaaggcatt cgcgccaccg gtgccaacaa actcgaagag atcctctacg
     4021 gcgtgctgcc acaggtgatg ccgctgctga tctcctactc cctctatcgc ttcgaatcca
     4081 acgtccgctc ggcgaccgtc gtcggcatgg tcggcgcggg cgggatcggc gtcacgctgt
     4141 gggaagcgat tcgcggtttc cagttccagc aaacctgcgc cctgatggtg ctgattatcg
     4201 tcacggtcag cctgctggat ttcctctctc aacggttgcg taagcacttt atctgattag
     4261 cgaggcattg atagctatgc acttgtctac acatccgacc agctacccga cacgctatca
     4321 ggagatagcc gcaaaacttg agcaggagct tcgtcaacac taccgctgcg gcgactatct
     4381 tcccgccgag cagcaactgg cagcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc
     4441 catcgaccaa ctggtggaaa aaggctgggt acagcgccgt cagggcgtcg gcgtactggt
     4501 gctgatgcgc ccgttcgatt acccgctcaa cgcccaggcg cgttttagcc agaacctgct
     4561 ggatcagggg agccatccca ccagcgaaaa actgctgtct gtgctgcgcc cggcctccgg
     4621 tcacgttgca gacgcactgg ggattaccga gggcgagaac gttatccacc tgcgcaccct
     4681 gcgccgggtc aacggcgttg cgctctgttt aatcgaccac tacttcgcgg acctcgccct
     4741 ctggccgacg ctgcaacgct tcgacagcgg ctcgctgcac gattttctgc gcgagcaaac
     4801 ggggattgcg ctgcgccgca gccagacgcg gatcagcgcc cgccgcgccc aggccaaaga
     4861 gtgccagcgt cttgaaatcc cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca
     4921 ccgtgacggt gaaagcagcc cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat
     4981 tgaatttact atggagcact gaatgcacgc agataccgcg acccgccagc actggatgtc
     5041 cgtactggcg cacagccaac cggcagaact ggcagcacgc ctgaacgcgc taaacatcac
     5101 cgccgactat gaggtgatcc gcgccgctga aactggcctg gtacagattc aggcgcggat
     5161 gggcggcacc ggcgaacgct tttttgccgg cgacgccacg ctgacccgcg ccgccgtacg
     5221 tctgactgac ggcaccctcg gctacagctg ggtgctgggg cgcgacaaac agcacgccga
     5281 acgctgcgcg ctgattgacg cgctgatgca gcaaacccgc cattttcaaa acttatcaga
     5341 aacgcttatt gccccgctgg acgctgaccg tatggcacgc attgccgcac gccaggccga
     5401 agtgaacgcc agccgggtcg acttctttac gatggttcgc ggagacaacg catgaccctg
     5461 gaaaccgctt ttatgcttcc cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag
     5521 gccatgagcg agccgggcgt gattgtcgcc ctgcatcagc tcaaacgcgg ctggcagccg
     5581 ctgaatatcg ccaccaccag cgtgctgctg acgctggccg ataacgacac gccggtgtgg
     5641 cttgccgcgc cattgagcaa cgatatcgtc agccagagcc tgcgttttca taccaacgcg
     5701 ccgctggtca gccagccgga acaggcgacc ttcgcggtaa cggatgaggc gatttccagc
     5761 gaacagctta acgccctttc caccggcacc gccgttgcgc cggaagcggg cgcaacgctg
     5821 attttacagg tcgccagcct gagcggcggg cgcatgctgc gtctcaccgg cgcgggtatt
     5881 gccgaagaac gaatgatcgc cccgcagctg ccggagtgca ttctgcacga actcaccgag
     5941 cgcccgcacc cgttcccgct cggcatcgac ctgatcctga cctgcggcga gcgcctgctg
     6001 gctgttccgc gaaccactca tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg
     6061 agaaggcgat cgacgccgcc cacgccctgc aagagagccg acgccggggc gataccgatt
     6121 tgcctgaact gagcgtcgcc cagattgaac agcagcttaa cctcgcagta gatcgcgtga
     6181 tgaccgaagg cggcattgcc gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg
     6241 gcgataacgt cgaagcaatt ttcctgctgc gcgcctaccg caccacgctg gcgaagcttg
     6301 cggtaagcga gccgctcgac accacggaga tgcgtctcga acggcgtatt tcagcggttt
     6361 ataaagacat tcccggcggc cagctgcttg gcccaaccta cgactacacc catcgcctgc
     6421 tcgatttcac cctgctggca aacggcgaag cgccgacgct gaccaccgcc gacagcgaac
     6481 aacagccgtc gccgcacgtt ttcagcctgc tggcgcgtca ggggctggcg aagtttgaag
     6541 aggatagcgg cgcacagccg gatgacatca cccgcacgcc accggtttac ccctgctcac
     6601 gttcttcccg cctgcaacag ctcatgcgcg gcgacgaagg ctatttgctg gcgctggcct
     6661 actccaccca acgcggttac gggcgcaatc acccgttcgc gggtgaaatc cgcagtggtt
     6721 acatcgacgt gtcgattgtg ccggaagagc tgggatttgc ggtgaacgtc ggcgaactgc
     6781 tgatgactga gtgtgaaatg gtcaacggtt ttatcgaccc accggatgaa tctccgcact
     6841 tcacgcgcgg ctacgggctg gtgttcggca tgagcgagcg caaagcgatg gcgatggcgc
     6901 tggtcgaccg cgctctgcaa gccccggagt acggcgagca cgcgacaggc ccggcgcagg
     6961 atgaagagtt cgtgctggca catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc
     7021 tcaaactccc gcactacgtc gatttccagg ccgaactgga gctactcaaa cgtctgcaac
     7081 aggagcagaa ccatggctaa tctgagcggc tacaactttg cctacctcga cgagcagacc
     7141 aaacgcatga tccgccgcgc catcttaaaa gcggtggcaa tccccggtta tcaggtgccg
     7201 tttggcgggc gcgagatgcc aatgccgtac ggctggggaa ccggcggcat tcagcttacc
     7261 gccagcgtga ttggcgaaag cgacgtgctg aaggtgattg accagggcgc ggatgacacc
     7321 accaacgccg tgtcgattcg caacttcttt aagcgcgtga ccggggtaaa caccaccgag
     7381 cgcacggacg atgcaacggt catccagacg cgtcaccgca tccccgaaac gccgctgacc
     7441 gaagatcaga tcattatttt ccaggtgccg atccccgaac cgctgcgctt tatcgagccg
     7501 cgcgaaacgg aaacccgcac catgcacgcg ctggaggagt acggcgtgat gcaggtgaag
     7561 ctgtatgaag atatcgcccg cttcggtcat atcgccacca cctacgccta tccggtgaag
     7621 gtgaacgggc gctacgtaat ggacccgtcg ccgatcccga aattcgataa cccgaaaatg
     7681 gacatgatgc ccgccctgca actgttcggc gccgggcgtg agaagcgcat ctatgcggtg
     7741 ccgccgttta cccgcgtgga aagcctcgat ttcgacgatc acccgttcac cgttcagcag
     7801 tgggatgagc catgcgccat ctgcggatcg acccacagct atcttgatga agtggtgctg
     7861 gatgacgccg gaaaccgcat gtttgtctgc tccgataccg attattgccg ccaacagagc
     7921 gaggcaaaaa gccaatgaat caaccgttac tttcggtcaa taacctgacc cacctttacg
     7981 cgccgggcaa aggctttagc gatgtctctt ttgatttatg gccgggggaa gtgctgggca
     8041 ttgtcgggga atccggctcc gggaagacca cgctgctgaa atcaatttcc gcgcgtctga
     8101 cgccgcagca gggggagatt cactatgaaa accgttccct ctatggcatg agcgaggccg
     8161 accgccgtcg cctgctgcgc accgaatggg gcgtggtgca tcagcatccg ctcgacggcc
     8221 tgcgccgcca ggtgtcggca ggcggcaata tcggcgagcg gctgatggcg accggggcac
     8281 gtcattacgg cgatattcgc gccaccgcgc agaagtggct ggaagaggtg gaaattcccg
     8341 ccaaccggat cgacgacctg ccgaccacct tttccggcgg catgcagcag cgtttgcaga
     8401 ttgcccgcaa cctggtgacg catccgaagc tggtatttat ggacgagcca accggcgggc
     8461 tggatgtgtc ggtgcaggcg cgcctgctcg acctgctgcg cggcctggtg gtggagctga
     8521 acctcgcggt ggtgattgtc acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt
     8581 tgctggtgat gaagcagggg caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg
     8641 acccgcatca tccgtataca cagctgctgg tgtcatcggt tttgcagaat tgagccggtg
     8701 ccggatgcgg cgtaaacgcc ttatccggcc tacaaatgcg ctccccgtag gtcggataag
     8761 acgcgtcagc gtcgcatccg acacccgaac cacgaggcga aaaatgatta acgtacaaaa
     8821 cgtcagtaaa accttcatcc tgcatcagca aaacggcgtg cgcctgcccg tcttacaacg
     8881 cgcctcactc accgtcaacg cgggcgaatg cgtggtgctc cacggccact ccggcagcgg
     8941 caaatcaacc ctgctgcgct cgctgtacgc caactatctg cccgacgaag ggcaaatcca
     9001 gatcaaacac ggcgacgaat gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga
     9061 gatccgcaaa accaccgtcg gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc
     9121 agcactggaa gtggtgatgc agccgctgct cgataccggc gtaccgcgtg aagcctgcgc
     9181 cgctaaagcc gcgcgtctgc tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc
     9241 gccatcgacc ttctccggcg gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt
     9301 cgactacccc attctgctgc tcgacgaacc taccgcctcg ctggacgcta aaaacagcgc
     9361 cgccgtagtg gaactgatcc gcgaagccaa agcccgtggc gcagccatcg tcggcatttt
     9421 ccatgacgaa gccgtacgca atgatgtcgc cgaccgcctg cacccaatgg gagcctcttc
     9481 atgattatca ataacgttaa actggtgctg gaaaacgaag tggtgcacgg ttcgctggag
     9541 atgcaggatg gtgaaatccg cgcctttgcc gaaagccaga gccgcctgcc ggaggcgatg
     9601 gacggtgaag gcggctggct gctgccggga ttgattgagc tgcataccga taacctcgat
     9661 aaattcttca ccccgcgccc gaaagtcgac tggcccgccc attctgcgat gagcagccac
     9721 gacgcattaa tggtggcaag cggcatcacc accgtactgg atgcggtggc gattggcgac
     9781 gtgcgcgacg gcggcgatcg gctggagaat ctggagaaga tgatcaacgc catcgaagag
     9841 acgcagaaac gcggcgtcaa ccgcgccgag caccgcctgc acctgcgctg cgagctgccg
     9901 catcacacca cgctgccgct gtttgaaaaa ctggtgcagc gcgagccggt gacgctggtg
     9961 tcgctgatgg accactcgcc gggccagcgc cagttcgcca accgcgagaa gtaccgtgaa
    10021 tattatcagg gcaaatactc gctcactgat gcgcagatgc agcagtacga agaagagcaa
    10081 ctggcgctcg ccgcacgctg gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccgc
    10141 gcgcgaaaaa ttgcgcttgc cagccacgat gacgccaccc acgcccacgt tgccgaatcc
    10201 catcagcttg gcagcgtgat cgccgaattt cccaccacgt tcgaagcggc agaagcctcg
    10261 cgcaagcatg gtatgaacgt gctgatgggc gcgccaaata tcgtgcgtgg cggttcgcac
    10321 tccggcaacg tggcggccag tgaactggcg cagcttggcc tgctggatat cctctcttcc
    10381 gactactacc ccgccagcct gctggatgcg gcgttccgcg tggccgatga ccagagcaac
    10441 cgctttacgc tgccgcaggc ggtgaagctg gtgactaaaa atccggcgca ggcgcttaat
    10501 ctccaggatc gcggggtgat tggcgagggc aaacgcgccg acctggtgct ggcgcatcgc
    10561 aagggcaacc acattcatat cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga
    10621 cgggaaaact gatttggtta atggggccgt ccggctccgg gaaagacagc ctgctggcgg
    10681 aactccgcct acgggaacaa actcagttac tggtggcgca tcgctacatc acgcgcgacg
    10741 ccagcgctgg aagcgaaaac catatcgccc tgagcgagca ggagtttttt acccgcgcgg
    10801 ggcaaaacct actggcctta agctggcacg ccaacggcct gtattatggc gtcggcgtcg
    10861 agatagacct ctggctgcac gccggattcg acgtgctggt caacggctca cgcgcccatc
    10921 tgccgcaggc gcgggcacgc tatgcatcgg cactgctgcc catctgctta caggtgtcgc
    10981 cagagatcct gcgccagcgc ctggaaaacc gtggtcgtga aaacgccagt gaaattaacg
    11041 cccggctggc gcgcgccgcc cgctatactc ctcaggattg ccatacgctc aacaatgacg
    11101 gcagcctgcg ccagtcggtc gacacgctgc tgacgctgat tcatcagaag gagaaacacc
    11161 atgcctgctt gtgagcttcg cccggccacg cagtacgaca ccgacgcggt ttacgcgctg
    11221 atttgtgagc taaaacaggc ggagtttgac catcacgcgt ttcgcgtggg ctttaacgct
    11281 aacctgcgtg acccaaacat gcgctaccat ctggcgctgc tggatggcga agttgtcggt
    11341 atgatcggcc tgcatttgca gtttcatctg catcatgtca attggatcgg cgaaattcag
    11401 gaactggtgg tgatgccgca ggcgcgcggt ctgaacgttg gtagcaagtt gctggcgtgg
    11461 gcagaagaag aagcccgcca ggccggggcc gaaatgaccg agctttcgac caacgtgaag
    11521 cgccacgacg cacaccgttt ctatctgcgc gaaggctacg agcagagcca cttccgcttc
    11581 acgaaggcgc tgtaacatga gcctgaccct cacgctcacc ggcaccggcg gcgcacaggg
    11641 cgttccggca tggggctgcg agtgtgcggc ctgcgccaga gcgcggcgct caccgcacta
    11701 tcgccgccag ccatgcagcg gcgtagtgaa gtttaacgag gcgatcaccc tgatcgacgc
    11761 cgggcggcac gatctcgccg atcgctggtc gccaggatcg ttccagcagt ttttgctgac
    11821 gcactaccat atggatcacg tccaggggct gtttccgctg cgctggggcg tgggcgatac
    11881 gatcccggtt tacggcccgc cggatgacca gggttgcgac gatctgttta aacatccggg
    11941 cctgcttgat ttcagccaca cagtggaacc gtttgtggtg tttgatttgc aggggttgca
    12001 ggtcacgccc ctgccgctca accactcaaa actgaccttc ggttatctgc tggaaacggc
    12061 gcacagccgg gtggcgtggc tgtctgacac cgctggcttg ccggaaaaaa cgctgaaatt
    12121 tttacgcaat aatcagccgc aggtaatggt gattgattgc agccacccgc cgcgcgagga
    12181 tgcgccacgt aatcactgtg atttaaacac cgtgctggcg ctgaatgaag ttatccgctc
    12241 gccgcgggtg attctgacac acatcagcca ccagtttgat gcgtggctga tggagaacgc
    12301 gctgccgccg gggtttgaag ccgggtttga tgggatggag attggggtgg cgtgatgaga
    12361 gggaatgtgc gcgctggccc cctcacccta accctctccc cagaggggcg atgcctgctc
    12421 catacccaac atcatcgccc atactcatct tccattctcc gctcttcatc ctccagctgc
    12481 cgacgctcct gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac
    12541 tggcggcggc ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagcca
    12601 tcgtcgcgcg aatcttcata atctcgccca cggtcagggt tataagcgtc gttaatcgcc
    12661 tgctgaatat tgccaatggt gtcgtcgata atatcggcct gggccggtac ggtggtcagc
    12721 gtgagcaggg tgaaaaaaaa gagcgtggag tggcgtttca taaactcggc ctcgcaacaa
    12781 agttaataca aggtaggcaa aggtgcctgt tacgagtaga ggaggattct caattctctt
    12841 gccccagcgc ctgcgccaac tgcgcacggg taaagggttt gcgcaacagc gccacgtctg
    12901 gcagcgcggg gttatggctg gggcgtaaat cctgaccgct gatgagtagc agcgtcaggt
    12961 gcgggtaaag tttgcgggcc gcattgacca cttctgcgcc gctcatgccg ccggggagca
    13021 ttaaatcgct gataaaaata tcgatttctg ctgatgccgc cagcagatcc agcgcctgtt
    13081 cgccgctgct tgcttccagc gtcaaatagc ccagcaggtg caactgttcg cagatggtct
    13141 ggcgcaccgc tgcttcatct tccagcacta acaccagttt atcgccgctg acagaagcct
    13201 gttctactgc cgctagcgat aggtttttcg ccgccgtcag cgcgcgggga agctgtaatc
    13261 tgaccgttgt cccctgcccc ggcgcgcttt caatttcaac gcgcccaccg gactggcgca
    13321 caaaaccata gaccatcgac aaacccagcc cgctgccgct gcccgtctgt ttggtggtaa
    13381 agaatggctc aaatacctgg cttttcaccg cctgcgacat accgctgcca tgatcgatga
    13441 cctccagtgc caccatgtcc tgacgctgcc cactgctgcg agtaacgcgc tgattccagg
    13501 tacgaatttt gatgattccc gtctgccctt ccatcgcgtc acgggcgttc atcaccaggt
    13561 tgatgatggc attttccagt tggctgatgt caatccacgc gggccacgcc ggagactgcg
    13621 cttcaatctc cagcgccagc gttgccggta aggagtggcg cattaattcg cttaactctt
    13681 ccagcaatgt tttcagttca acggcttgcg gatgtaagga ctgcttgcgg gaaaacgcca
    13741 gcagtcgttg tgtcagtagc gccccacgtt cggcagcttg cagcgcccgc tgtattcgcg
    13801 gcgcgtctgg cgagtccgga ttcaccagct ccaggctgcc aataatcacc gccagcagat
    13861 tattaaaatc atgggccagc ccgcccgtca gttgcccgac cgctttcagt ttctggctgt
    13921 gtaatagcgc ctcttccaac ccctgccgct cagtttgggc aatctccatc tgcgtggtct
    13981 tctcttttag cagccgtgag gttcgttcga gtgactcggt attgcgggca aaaacgttaa
    14041 aggcgcgcgc cagttcaccc aactcatcac gccgctgtaa cgccggaaaa gtgacgtccg
    14101 cttcgccgta cgccaggcgg ctcatggcgc gggaaatcgc cgtcaggtta gtgccgaggt
    14161 tgcgatagat ataccagccg gcaaacgcgg tgatcaccac cgctaacagg gcgaaaatca
    14221 tgatcaccct ctcgccggag cgcaaatcct ggtaactttg cgccacgcgc tgaccggaga
    14281 gcgtcgccac ctgttcggcg taacggttga tgtcgccatt gaggatcgcc accagcgctt
    14341 tgatgtgaaa catataccag ctgattgcca gatcgctttg ctccagagcg gcagaaagcg
    14401 gcgcgagttt acgcatttca tcattgaaat cattgagtaa cgtattaacc aatggcatat
    14461 cgctgtgggt cggcaacgca gacattaccc cgaccagttg atgaataatc gcctgcggcg
    14521 cgggggtggc aatagcggca acaatcagtc ggttcatctc gttaagcaaa atatcatctt
    14581 gcgcgtgagt gactttttgc agatgccgca gataacttaa gttttgatac agcgaactta
    14641 acagcgcgtt gcgctccaga tgccgccttt gcccgcgctc cagcataccg ccgacgctgg
    14701 tttgcagttc gttgctacgc gtgatgattc gcgtcaccag cccggcttcc tgctgcgcta
    14761 aaggcgcgtt cgccagttgt gcaagcgagc tttttaatgc ccgctgtgtc tcctgcaacc
    14821 gctcggcttc gcctttatat tccagcgctc ccaccacctg cgacaatcgc accgccgccg
    14881 ttgccacgtt tgccgtgtcg cgcgccagat tcaggctgcc ggtcatatca tccaccgttt
    14941 gctgctgcac cagctcctgc atctggctgg catgacgaaa gcccattacc gccacgccgc
    15001 tgaccatcaa cgtcaccgcc accaccagca aattcaaaag cagcaatcgc ccgcgcgcgc
    15061 tggcaaaaaa atgacgtccg gggtgcgcca tatcacgctc cgtgaagaaa aatcgcaata
    15121 tgacaaatat gaacggcttc tgacattttg catttacttt cctgtgatgg agtgctgata
    15181 tcgacaacct cgcaggagtc caccatgaac agcacgccag ttctggagat gcgcaatatt
    15241 gccaaagcct tcggcaaatt ttatgcgctc aaaggggtgg atttgacggt ctaccctggc
    15301 gagatccatg ccctgatggg ggaaaacggc gcgggaaaaa gcacgctgat gaaggtgctg
    15361 gcgggggcgt ataccgccac cagcggcgag atcctcatag acggcaagcc ctttcacatt
    15421 cgcacgccaa aagatgcctt aagcgccggt attacgctga tttatcagga gatgcagctg
    15481 gcaccgaatc tgtcggtggc agaaaatatt tttctcggca gcgagctttc ccacggcggg
    15541 ctggtgcagc gtaaagagat gctagtgcag gcgcaaaaag tgatcgaccg cctcggcgcg
    15601 cagtttaacg ccagcgataa ggtcatgacg ctgaccattg ccgagcaaca gcaggtcgaa
    15661 atcgcccgcg cactacatcg caacagccgc attctggtga tggacgaacc caccgctgcc
    15721 ctctcctccc gtgaaactca ccgtctgttt gaactgatta tgcggctgcg cgatgaaggg
    15781 atggcgatta tctacatcag ccaccgtatg gcggaagtgt atgagctttc cgatcgcgtc
    15841 agcgtgctgc gcgacgggca atacgttggc agcctgaccc gcgataacct caatgccggg
    15901 gagctggtgc ggatgatggt cggcaggcca ctgagcgatc tgttcaataa agagcgcgat
    15961 atcccgctcg gtaaagcccg cctgaatgtt cacctcctga ccgacggcgg caaagtccag
    16021 ccgagtagcc tgctggtgcg ttccggcgaa attgttggcc tcgccggact ggtgggtgcc
    16081 ggacgttccg aactggcgca gttgatcttc ggcgtgcgga aagcgacagg cggaatgatt
    16141 gaagtcgatg gtgaaccggt ggtgatccac tccccgcgcg aagccatcga tcttgccatt
    16201 ggttttctca ccgaaaaccg caaagaacaa ggcttattcc ttgaaatggc agcagccgaa
    16261 aacatcacca tggcaaccct ggagcgcgat gcccgctggg gaatgctcaa tcgcaaaaaa
    16321 gcgcaaacca tttccgatga cgccattaag ttgctcaaca ttcgcgtgcc tcatgcccag
    16381 gtacgcgcgg gcgggctttc cggcggcaat cagcaaaaac tgttgatctc ccgctgggtg
    16441 gcgattggcc cgcgcatttt actgctcgat gaacccaccc gcggcgtgga cgttggcgcc
    16501 aaaagtgaga tctaccggat catgaacgag atggcgcgca agggcgtggc gatcctgatg
    16561 atctccagcg aactgccgga gatagtggga atgagcgatc gcgtctatgt gatgcgcgaa
    16621 ggcagcattg ccggtgagtt aaacggcaaa aacatcaccc aggaaaacat tatgacctta
    16681 gcgactggcg tcaacgacgc ccattcccag gcggtaacct catgacgacc tctgaaaaaa
    16741 cctctgccaa aaaaatactg atgagcgatc tgatgcaaac ggtcggcatc ttgcccattt
    16801 tgattctgat cgtggcggta tttggcttta tcgcgccaaa cttttttacc gaaagcaacc
    16861 tgctgaatat tacccgccag gcatcgatca acattgttct ggcggcgggg atgaccttca
    16921 tcattttgac cggcgggatc gatctttccg tgggttcgat cctcggcacc acagcggtgg
    16981 cggcgatggt cgtctccctt agccctgagt tcgccatgct gtcgatcccg gcagcgctga
    17041 tgctcggcct gctgctgggg ctgttcaacg gcgcgctggt ggcctttgcc ggattgccac
    17101 cgtttatcgt tacgcttggc acctatacgg cgctgcgcgg ggcggcgtat ctgctggcgg
    17161 acggcaccac ggttatcaac tccaacatta atttcgagtg gataggcaac aactatctcg
    17221 ggccggtccc atggctggtg gtgattgcgc tggcggtgat cgtggtgtgc tggtttattc
    17281 tgcgccgcac cacgcttggc gttcatatct acgccgtcgg cggcaatatg caggcggcgc
    17341 gtcttaccgg cattaaggtc tggctggtgc tgctgtttgt ttatggtatg agcggcctgc
    17401 tttcagggct gggcggaatt atgagcgcct caaggctgta cagcgccaac ggcaacttag
    17461 gtatggggta cgagctggac gccattgcgg cggtgatcct cggcggcacc agttttgtcg
    17521 gcggcatcgg caccatcacc ggcacgctgg tcggcgcgct aattatcgcc acgctaaaca
    17581 acggcatgac cttaatgggc gtctcttact tctggcaact ggtgatcaaa ggggcggtga
    17641 tcatcattgc ggtgctgatc gacaaatacc gtacccgaca ccatcaaagt gcataaacac
    17701 atgaggaata cagcatgaga ttgaagccct tagtgaccgc gctctgtgca ggcgcgctgc
    17761 tggccgcagc accgttcgcc caagcgaaag aactgaaatc gataggcgta acggtgggcg
    17821 atctggcgaa cccgttcttc gtgcagatca ccaaaggcgc ggagctggaa gcacgcaaac
    17881 tggcaggcga taacgtcaaa gtgacgctgg tttccagcgg ctacgatctg ggtcagcagg
    17941 tgtcgcagat agacaacttt atcgccgcta acgtcgatat gattatcctc aacgctgccg
    18001 attcaaaagg gatcggcccg gcggtgaaac gggcgaaaga tgccgggatc gtagttgtcg
    18061 cggttgacgt agcggcagaa ggtgctgatg ccaccatcac ctccgataac acccaggcgg
    18121 gcgaaatggc ctgtaaatac atcaccgatc gcctgaaagg caaaggcaat gtggtgatca
    18181 tcaacggccc gccggtatcc gccgtgcaaa accgcgtcga aggatgccag actgaattta
    18241 aacgccatcc tgacatcaaa gtgctctctg acaaccagaa cgcgaaaggc agtcgtgaag
    18301 gcgggctgga ggtaatgacg ggactgctgg cggcaaatcc gaagatcgac ggcgtatttg
    18361 cgattaacga tcccaccgca atcggtgccg atctggcagc aaaacaggcg caacgtaacg
    18421 agttctttat tgtcggtgtg gacggaagcc cggacgccga agaggcgctc aagcgtgaga
    18481 attcgttgtt tgtcgctact ccggcgcaag atccccaggt gatggcagcg aaagcggtgg
    18541 aaattggcta cgacattttg cagggcaaac ctgcgccgaa agagccggtg ctgatcccgg
    18601 tgacgatgat cgataaaaac aacgtcggca attacaaagg ctggacggtg aaataagtcc
    18661 acacgccggg cggcgcagct gcccggtcac cgctaaggag aacgctttcg atgaaacgct
    18721 ccgctataaa cgacattctc ggccatacac ggcaattctt ttcgcaacat gacgtacatc
    18781 tgccgccctt tgccagtttc tcgcctgctc aatggcagca gctcgacacc gccgcgtggg
    18841 aggaggtttt tgatcttaaa ctcggctggg atgttaccgc ttttggtcgc aacaactttg
    18901 cagcacacgg gctgacgctg tttaccctac gtaacggctc agcaaaagga atgccgtatg
    18961 tgaaatgtta cgccgagaag atcatgcatg tgcgcgatgc gcaggtcacg ccaatgcatt
    19021 ttcactggcg taagcgtgag gacattatca atcgcggcgg cggtaattta attgttgaac
    19081 tgtggaatgc cgatagtaac gagcaaacgg ctgatagtga cattacggtg gtgatagacg
    19141 gctgccgcca gaaacatact gcaggcactc agttgcgcct gtctcccggc gaaagtatct
    19201 gcctgccgcc cggcctgtat cacagctttt gggcggaaac cggctttggc gatgttctgg
    19261 ttggcgaagt ctcttctgtc aatgacgacg accacgataa ccacttttta cagccactgg
    19321 accgctacaa cctgattgac gaagacgaac ctgcacagtt ggtattgtgt aacgaatacc
    19381 gccagttccg ctgaataagg ataacgttat gccattgatt tctctcgctg aagggctggc
    19441 acatgcccgc gaacatcact attcgttagg tgcgtttaat gtgctggact cgcacttttt
    19501 acgcgccttg tttgctgctg ccagacaaga acgttcgccc tttattatca acattgccga
    19561 agtacatttt aaatatatct cgctggattc cctggttgag acagtgaaat ttgaagcggc
    19621 gcgtcatgcc attccggtgg tgttaaatct cgatcacgga ctgcattttg aatcggtggt
    19681 gcaagcgttg cggctgggat tcagctcggt aatgtttgat ggctccacgc tggggtatga
    19741 agaaaatgtc cgccagacgc gggaagtggt gaaaatgtgc catgccgttg gcgtatcggt
    19801 agaagctgaa ctgggcgcgg tgggtggcga cgaaggcggt gcgctctatg gtcatgccga
    19861 tgaatccctg tttaccgatc cgcaactggc gcgtgatttt gtcgaccgta ccggcattga
    19921 cgcgttggcc gtggcgattg gcaatgcaca cggcaaatat cgcggcgaac cgaaactcga
    19981 tttcccgcgc ctcgacgcca tccgccagca agcggctatc ccgctggtgt tacacggcgg
    20041 ctcagggatt agcgatgccg atttccgccg cgccatcgaa ctgggtatcc acaaaatcaa
    20101 tttctacact ggcatgtcgc aagcagcgct ggctgccgtt gaggcccgaa tggcgcaccg
    20161 tcagccgatg tatgacgagt ttgccgagtt actgctggca gtggaagccg ccatcagcga
    20221 tacggttgca caacagatgc gtattttctg tagtgcgggg caggcatgat ggagcgtaaa
    20281 gggattatcg ccgcaggcaa tatgctggtt gaccacgtcc atcagattat ccagtggcca
    20341 gagcgcggct ggctggcgga gatcacccac agcgaacgct ccaccggcgg tgcgccgctt
    20401 aacgtcctgc tgacgctggc gaaaatgcgc accgggctgc cgttacaggc ggttgggcta
    20461 atcggtcagg acagcgacgg cgattacatc atggcgatgc ttgagcaata tcacgtcaat
    20521 cgtcagcatg tgcagcgcac tacctttgcc ccgacgtcga tgtcgcaggt gatgacagac
    20581 ccaagcgggc agtggacgtt tttccattcg ccgggtgcaa acagactttt ggatcttccg
    20641 gcgttcgatc agctcgataa caccatgaag attttccatc tcggctattt actgttgctc
    20701 gacagcctgg atatgccgga cgatgtttac ggcacccgta gcgcacgcct gctggcgcaa
    20761 atgcgtgata ctggctttga aacctcactc gacctggtct cccgtaaggg cgatccgcgt
    20821 tatcagccgc tggtgctgcc cgcgttgcgc catctggatt atctggtaat taacgaactg
    20881 gaggccgggg agtttagcgg gctggaaatt cggttaccca acggcgaacc gcatatcgcg
    20941 catattgctg ccgccgcgcg tgaactgtta gatgcgggcg tgcgccagcg ggtggtgatc
    21001 cactgcccgg aaggggcgtg gggagagacc ccagaacagg gcggcgtgtg gatcccttcg
    21061 caaaagttag aacaacggga aattatcggc agcgtcggcg cgggcgatgc attttgtgcc
    21121 ggatttttat atggctgcca tgaacgctgg acgcttaccg agagcatcaa actggcacac
    21181 gcctgcgcgc gggcaagcct gttatgcgcc aatgccatcg acggggcgaa aacgctggaa
    21241 gagctgcaaa cagaaatgtc ggattaatgc acgtcggcag cgaagacata ccccagaccg
    21301 cgcagagttt taatcaacat cggctgatgc gggttgagtt cgattttgcg tcgtaatcgc
    21361 atgattaata cgtcgatggt gcgatcaaac acctccacgc tgtcgctgtg ggtgaacgcc
    21421 agcaactgct cgcggctcaa tacccgacgc gcgttttgcg ccagagccag taacaagcca
    21481 tattcgccct gagttaactc gacatgttgc tgaagcggat tgtgcaactg gcagcgcatg
    21541 gtatcgagat accagccatt gaatgacagc ccggcgcttt tctcgtcatt aaccgccagc
    21601 gcaccaaccc gtcgcaacac cgctttcagt cgtgcgacca ccacccgcgg ggtgaacggt
    21661 ttgcagatgt aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactgccc
    21721 atgccggtca acatcaccac cggcaatgtc ggacgcagtt tctggatttg ctgcaatacc
    21781 ctcaggccgt tggtatcggg tagcatcata tccagcacca ccagcgcaat gtcaggatcg
    21841 ccctcaatac gcaatatggc ctcctgtccg gtatggcaga ctgaaaccgt aaagacatgt
    21901 tcacttaaca cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg
    21961 acgggtttca tggttacact ccgcatcatt catctgatta atcattgtgc gtacttcttg
    22021 tcgaagtgaa agctgaaagt aatataaata ataaaaatat aagtcaggtc acattattga
    22081 tgatagtaat aaatatgccg tacttttcca tcttcagcac aaat
//
LOCUS       DAZGCQ010000085        21653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000085 DAZGCQ010000000
VERSION     DAZGCQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.085"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..661
                     /gene="xylF"
                     /locus_tag="ACHK07_003549"
     CDS             <1..661
                     /gene="xylF"
                     /locus_tag="ACHK07_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8103834.1"
                     /translation="YDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGS
                     PVDNNAKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKID
                     AVVASNDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLA
                     NTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESE
                     L"
     gene            739..2280
                     /gene="xylG"
                     /locus_tag="ACHK07_003550"
     CDS             739..2280
                     /gene="xylG"
                     /locus_tag="ACHK07_003550"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO8103835.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            2258..3439
                     /gene="xylH"
                     /locus_tag="ACHK07_003551"
     CDS             2258..3439
                     /gene="xylH"
                     /locus_tag="ACHK07_003551"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO8103836.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSTAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            3517..4695
                     /gene="xylR"
                     /locus_tag="ACHK07_003552"
     CDS             3517..4695
                     /gene="xylR"
                     /locus_tag="ACHK07_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO8103837.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(4803..5627)
                     /gene="bax"
                     /locus_tag="ACHK07_003553"
     CDS             complement(4803..5627)
                     /gene="bax"
                     /locus_tag="ACHK07_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO8103838.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            5947..7977
                     /gene="malS"
                     /locus_tag="ACHK07_003554"
     CDS             5947..7977
                     /gene="malS"
                     /locus_tag="ACHK07_003554"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8103839.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            8155..9408
                     /gene="avtA"
                     /locus_tag="ACHK07_003555"
     CDS             8155..9408
                     /gene="avtA"
                     /locus_tag="ACHK07_003555"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO8103840.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(9559..10032)
                     /gene="ysaA"
                     /locus_tag="ACHK07_003556"
     CDS             complement(9559..10032)
                     /gene="ysaA"
                     /locus_tag="ACHK07_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8103841.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(10134..10274)
                     /locus_tag="ACHK07_003557"
                     /pseudo
     CDS             complement(10134..10274)
                     /locus_tag="ACHK07_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(10276..11091)
                     /locus_tag="ACHK07_003558"
     CDS             complement(10276..11091)
                     /locus_tag="ACHK07_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8103842.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            11317..12717
                     /locus_tag="ACHK07_003559"
     CDS             11317..12717
                     /locus_tag="ACHK07_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8103843.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNQLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            12728..14698
                     /locus_tag="ACHK07_003560"
     CDS             12728..14698
                     /locus_tag="ACHK07_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO8103844.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNISRVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIVVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(14828..15568)
                     /gene="yiaT"
                     /locus_tag="ACHK07_003561"
     CDS             complement(14828..15568)
                     /gene="yiaT"
                     /locus_tag="ACHK07_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO8103845.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            15692..16666
                     /gene="yiaU"
                     /locus_tag="ACHK07_003562"
     CDS             15692..16666
                     /gene="yiaU"
                     /locus_tag="ACHK07_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8103846.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDISDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(16663..17799)
                     /gene="yiaV"
                     /locus_tag="ACHK07_003563"
     CDS             complement(16663..17799)
                     /gene="yiaV"
                     /locus_tag="ACHK07_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8103847.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(17805..18128)
                     /gene="yiaW"
                     /locus_tag="ACHK07_003564"
     CDS             complement(17805..18128)
                     /gene="yiaW"
                     /locus_tag="ACHK07_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8103848.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            complement(18673..20211)
                     /gene="aldB"
                     /locus_tag="ACHK07_003565"
     CDS             complement(18673..20211)
                     /gene="aldB"
                     /locus_tag="ACHK07_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO8103849.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAYTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(20376..21527)
                     /gene="yiaY"
                     /locus_tag="ACHK07_003566"
     CDS             complement(20376..21527)
                     /gene="yiaY"
                     /locus_tag="ACHK07_003566"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO8103850.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         5402 a         5544 c         5372 g         5335 t
ORIGIN      
        1 ttacgaccgt atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa
       61 agtcggtgaa ctgcaggcaa aagccctggt cgatattgtt ccgcaaggta attacttcct
      121 gatgggcggc tcgccggtag ataacaacgc taagctgttc cgcgccggac aaatgaaagt
      181 gttaaaacct tacgttgatt ccggaaaaat taaagtcgtt ggtgaccaat gggttgatgg
      241 ctggttaccg gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa
      301 aattgatgct gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag
      361 cgcgcaaggt ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat
      421 taaacgtatt gctgccggta cgcaaactat gacggtatat aaacctatta cattgctggc
      481 aaatactgcc gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac
      541 ctcactgaat aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa
      601 taaaaacaac atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta
      661 agcgttacgc cccagcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt
      721 ggcttaggtg gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc
      781 ggcagtgtga aggcgattga taacgtcagc ttgcggttaa atgctggcga aatcgtctca
      841 ctctgtgggg aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat
      901 ccccatggct cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac
      961 atccgcgata ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa
     1021 gaattgaccg tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg
     1081 gattatgacc tgatgacgct acgctgtcag aagctgctcg cacaggtcag tttatccatt
     1141 tcacctgata cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc
     1201 aaggcactta ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact
     1261 gagcaggaaa cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc
     1321 tgtatttata tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt
     1381 attcgcgacg gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt
     1441 atcaccatga tggtcgggcg agagttaacc gcgctttacc ctaatgaacc acataccacc
     1501 ggagatgaaa tattacgtat tgaacatctg acggcatggc atccggttaa tcgtcatatt
     1561 aaacgagtta atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga
     1621 ctcgttggtg ccggacgtac cgagaccatt cagtgcctgt ttggtgtgtg gcccggacaa
     1681 tgggaaggaa aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc
     1741 atcgcccagg ggattgcgat ggtccccgaa gacagaaagc gcgacggcat cgttccggta
     1801 atggcggttg gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc
     1861 cagcttgatg acgcggcaga gcaaaaatgt attctggaat caatccagca actcaaagtt
     1921 aaaacgtcgt cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg
     1981 atcctcgctc gctgtctgtt acttaaccca cgcattctca ttcttgatga acccaccagg
     2041 ggtatcgata ttggcgcgaa atacgagatc tacaaattaa ttaaccaact cgtccagcag
     2101 ggtattgccg ttattgtcat ctcttctgaa ttacctgaag tgctcggcct tagcgatcgt
     2161 gtactggtga tgcatgaagg gaaactaaaa gctaacctga taaatcataa cctgactcag
     2221 gagcaggtga tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga
     2281 agtgaaattg gccgtaccga catccggtgg cttctccggg ctgaaatcac tgaatttgca
     2341 ggtcttcgtg atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga
     2401 tggtgcctac ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg
     2461 catcctcgcg gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg
     2521 ctcaatgatg gggctattag gtggcgtcgc ggcgatttgt gacgtctggc taggctggcc
     2581 tttgccactt accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg
     2641 atggtgggtc gcgtaccgca aagtcccttc atttattgtc actctcgcgg gcatgttggc
     2701 atttcgcggc atactcattg gcatcaccaa cggcacgact gtatccccca ccagcaccgc
     2761 gatgtcacaa attgggcaaa gctatctccc cgccagcacc ggcttcatca ttggcgcgct
     2821 tggcttaatg gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg
     2881 tttgcagtct ccggcctcta ccgcagtagt cggtcgccag gctttaaccg ctatcatcgt
     2941 attaggcgca atctggctgt tgaatgatta tcgtggcgtt cccactcctg ttctgctgct
     3001 gacgttgctg ttactcggcg gaatgtttat ggcaacgcgg acagcatttg gacgacgcat
     3061 ttatgccatc ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac
     3121 caaacttgcc gtgttcgcta ttaacggatt aatggtagcc atcgccggat taatcctcag
     3181 ttctcgactt ggcgctggtt caccttctgc ggggaatatc gccgagctgg acgcaattgc
     3241 agcatgcgtg attggcggca ccagtctggc aggcggcgtc gggagcgtgg ctggagcggt
     3301 aatgggggca ttcatcatgg cttcactgga taacggtatg agtatgatgg atgtaccgac
     3361 cttctggcag tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc
     3421 aaccaaacgc cgttcttgat tttgataaaa attttctcaa agccggtaac gtattaccgg
     3481 ttttgagttt ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc
     3541 acattactgt tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa
     3601 tatttacagg cgtcacaatc ggaatgggat attttcattg aagaagattt ccgcgcccgc
     3661 attgataaaa tcaaggactg gttaggagat ggcgtcattg ccgacttcga cgacaaacag
     3721 atcgagcaag cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac
     3781 cttgcagaaa gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa
     3841 agcgcatttt tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg
     3901 gaatcaagcg gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc
     3961 gaagaaaagt atcgcggagt ggtttatcag gggttagaaa ccgcaccaga gaactggcaa
     4021 cacgcgcaaa atcggctggc agactggcta caaacgctgc caccgcaaac agggattatt
     4081 gccgttactg acgcccgagc acggcatatt ctgcaagtat gtgaacatct acacattccc
     4141 gtaccggaaa aattatgcgt gattggcatc gacaacgaag aactgacccg ctatctgtcg
     4201 cgtgtcgccc tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa
     4261 ctgttgcatc gattattaga taaagaagaa atgccgctac agcggatttt ggtccctcca
     4321 gttcgcgtca ttgaacggcg ctcaacagat taccgttcgc tgaccgatcc cgctgttatt
     4381 caggccatgc attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactc
     4441 gatgcggtcg ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa
     4501 accatccatg ccatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca
     4561 accaccttgt cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc
     4621 tactctgttt ttaaaaaagc atatgacacg acgccaaaag agtatcgcga tgtaaatagc
     4681 gaggtcatgt tgtagcgatg atgagaattg tcggcgtcgc atcaggtaac tcagcacaac
     4741 cacctgatgc gattaaaagc cttatccgac cgacaagtcg gataaggctc gaggaaatac
     4801 aattacatat gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg
     4861 ttataactct tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc
     4921 acttcctgat cagctttacg cagctgcgca cgcgatttac ggaacgaaga gtaagccgga
     4981 tgcgtattga ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag
     5041 taccctttca ctttacctgg cgcattggta caacgtcctt tcatgcattt catgccgaac
     5101 aggttgttgt tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc
     5161 gtcgccacca tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt
     5221 tttcgcgtat taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca
     5281 cgctcagcag gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca
     5341 gtaatggctg cattttggct ggtaatgtaa ggcattacgg tccgaagaaa cgcctttttc
     5401 cttggtgttc cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg
     5461 cgagaatact cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt
     5521 tgactcgctg ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt
     5581 aacataagaa tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt
     5641 cggatttaat cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc
     5701 atatcaaaaa tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac
     5761 gtgttaacga ttcagattgg cgctaaatcg cagaaaatgt ggggtttatc gcaaaattca
     5821 gccgtttttt gcgcgagatc gctcaccctt gcttctcatc ctgtggactt accgctcagg
     5881 gatgagtttt gtttggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca
     5941 tccgccatga aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc
     6001 agctggactt ctccggggtt ccctgccttt agtgaacagg gaacgggaac atttgtcagc
     6061 cacgcgcagt tgcccaaagg tacgcgtcca ctaacgctaa attttgacca acagtgctgg
     6121 cagcctgcgg atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg
     6181 ccgcctcaat ggcgattgtt cagggacggc gaatatacgc tgcaaataga cacccgctcc
     6241 ggcacgccaa cattgatgat ttccatccag aacgccgccg aaccggtagc aagcctggtc
     6301 cgtgaatgcc cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cacttttccg
     6361 gaaggagccg ccgtacggga ttattacagc cagcaaattg cgatagtgaa gaacggtcaa
     6421 ataacgttac aacccgctgc caccagcaac ggtttactcc tgctggaacg ggcagaaact
     6481 gacacatccg cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt
     6541 ttcgaaaacg gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg
     6601 gaaataggta cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattatctc
     6661 cagcagctgg gcgtcaatgc tttgtggata agcgccccat ttgagcaaat tcacggctgg
     6721 gtcggcggcg gtacaaaagg tgatttcccg cattatgcct accacggtta ttacacacag
     6781 gactggacga atcttgatgc caatatgggc aacgaagccg atctacgaac gctggttgat
     6841 agcgcgcatc agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccggctat
     6901 gccacgctgg cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgag
     6961 ctgaaaaaaa cgctgggtga acgctggagt gactggaaac ctgccgccgg gcaaacctgg
     7021 catagcttta acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga
     7081 aaaaactgga tcagaacgga tatcggcgat tacgacaatc ctggatttga cgatctcact
     7141 atgtcgctgg cctttttgcc ggatatcaaa accgaatcaa cgaccgcttc tggtctgccg
     7201 gtgttctata aaaacaaaac ggatacccac gctaaagtca tcgacggctt tacccctcgc
     7261 gattacttaa cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcga
     7321 gtcgataccg ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc
     7381 gccgcgcttc gcgaatggaa aaaagctaac cccgacaaag cattagatga caaacctttc
     7441 tggatgaccg gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc
     7501 ttcgatgcga tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg
     7561 gcgcagatgg atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg
     7621 agctacctct cgtcgcatga tacccgcctg ttccgtgaag ggggcgacaa agcagcagag
     7681 ttattattat tagcgccagg cgcggtacaa atcttttatg gtgatgaatc ctcgcgtccg
     7741 ttcggtccta caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat
     7801 gttagcggta aatctgccgc cagcgtcgcg cactggcaga aaatcagcca gttccgcgcc
     7861 cgccatcccg caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc
     7921 tttgttcgtg agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt
     7981 ttccggcttc ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc
     8041 taatcagcgt tgcaggataa agcaccactc actcttcaac agaccgattt gcaccccagc
     8101 aaatgtagcg ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca
     8161 ttctcccttt ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat
     8221 ctgaacgacg gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag
     8281 atcccggaaa tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa
     8341 gcgactgatg cactgtgcaa ttacgacggt ccacagggga aaacggagct actcacactg
     8401 cttgccggaa tgctgcacga gaagttgggt tgggatatcg aaccacagaa tattgcacta
     8461 acaaacggca gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc
     8521 gatggtcggg tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac
     8581 gccggactgg aagaagatct gtttgtctct gcgcgtccga atattgaact gctgcctgaa
     8641 ggccagttta aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg
     8701 atttgcgtct cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg
     8761 aagcttgacg cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggt
     8821 gtcccgttcc cgggtatcat cttcagtgaa gcgcgcccgc tatggaatcc gaatatcgtg
     8881 ctgtgcatga gtctttccaa gctgggtcta cctggctccc gctgcggcat tatcatcgcc
     8941 aatgaaaaaa tcatcaccgc catcaccaat atgaacggca ttatcagcct ggcacctggc
     9001 ggtattggtc cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct
     9061 gaaacagtca tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc
     9121 cgctatttac cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg
     9181 ctatggttta aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc
     9241 ggcgtgctga tggtgccggg gcacaacttc ttcccagggc tggataaacc gtggccgcat
     9301 acgcatcaat gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg
     9361 aagattctgg cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat
     9421 gcgctacgct tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta
     9481 ggccggataa ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttta
     9541 gaaacgccgg aaaacgtttc aaacaggctg ctgccgtagc cgatgccgct gcactttctc
     9601 gacatccatg cactgcaacg cctttgtcgg gcaggcttca acacaggccg gtcccgtctc
     9661 ccgatgccag cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc
     9721 catcgcacca aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgattgttc
     9781 aacgaaaata tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg
     9841 tgcatcttca cactgatgac aggctactgc cgtggtccag ctgtggtctt taataacacg
     9901 aatacgggaa ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga
     9961 cactgcgcaa gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat
    10021 aaaccggttc atgatcactc ctttgcacta aaacagcaga gtgccagaga gcacgtccct
    10081 actgctttga tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg
    10141 atcgcgccca ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt
    10201 tcacgcagtg gtttcaggag atttttctcc cccacctgtt tcagacgtga tgtcgaaagc
    10261 gaaatcgaca cggcactacg aattgatatg ttccttgctg taacgccgct tccacgctgc
    10321 tggcgttaaa ccggtgtgtt tgcgaaaaat ctgccggaaa tagccaacgt cattaaaccc
    10381 gcacaacgcc gccacttccg tgagcgatcg cgcttcgcta attaacagct tttcggcttc
    10441 gcgcacccgc tggcgatgga ttgcttctgt taacgtcaga tgaaacaccc gccggtagac
    10501 gcgcccaaga taatcggcat tgcagtacag ctcgcgggcc agaagcgccg tagaaagcgg
    10561 gcgatgatat tgcgtgcgga tcagtttctg cgctttccag gcaagggtgg tgcctgcgcc
    10621 atcggcctgt tcatcaagac gcgcgtcacg ggtgagctgc tgcaaaatga gcagcacaat
    10681 aaactccagc gccggagtgc gttgatgctt ttcctgttcg cttaagaact ggcgaaacag
    10741 ggccaccagc gcctgcggat cgacaacctt cgtttgttgc ggcacgctaa gatgagtaag
    10801 ccagcgaggg tcgttgtccg gcgctttaac ctcaaaatgc agccagtaaa atttgagatc
    10861 ggcgggaaaa tcctccaccc caacatgttg tcggttcggc cagagtaata aactctctcc
    10921 cgcatcgacc agaaatatcc gttcttcttc ctgaatcgtt aatcgacctt tttcgacaaa
    10981 gataatttcc catgactcca gccgccttgc aggatgcctc cctacgccac gggaaataaa
    11041 taatccacca ttttgcactt taatcggtaa cgtcatggat aattcgagca ttgatatgta
    11101 atttcccgct tattcattaa tgattaaaca cattaccttt tgattattaa tcaaaaacat
    11161 taatttcacc tgatccattt cacactttta aattcaggtc ggaatcgtca ccttttttta
    11221 ctcttttctc gccttgttga tattcccgtt cacggcagaa ttaatcaggt catttcaata
    11281 aaactaaaaa ttgctcccta ttcacgagga atatctatga cctctacacc gattactacc
    11341 gctgatatcg ctaaaggtaa aattgacgat gcgttatctg tacgggaaaa aataggctac
    11401 ggcctgggtg acgcaggcgg cactgtaata acttgcctga tcatgaattt tctcaccttt
    11461 ttctacaccg acgtttttgg attaactccg gcgctggttg gcacgctgtt tattgcactg
    11521 cgcgtgtttg atgccatctc cgacccggtg atgggcgtca ttgccgaccg gacgcaaagt
    11581 cgctgggggc gctttcgtcc gtggcagcta tggattgcca ttcccatcgg cattatcggc
    11641 atcctgacgt tcaccgtgcc agatgccagc atgggagtaa aaatcgcctg ggcgttcggt
    11701 acttacctgc tcctttcagt cggttatacc gccatcaacg taccgtactg cgcgctgatc
    11761 aacaccatga ccacccgcca caatgaagtg atctcctgcc agtcctggcg attcgttctc
    11821 tgcggcgtag cgggatttct ggtttcggta ggcttaccgt ggatggtaga tctcttcggt
    11881 cagggcaacg ctgcacgcgg ctatcaactg ggcgtcgggg tattgtgcgc cattgccgtg
    11941 gtgatgttcc tgtgctgttt cttctgggtt cgtgaacggg tgccgctctc cacaatgggg
    12001 aaatttaccc tgcgcgaaca tcttgccggg ctgcggaaca acgaccaact gctgctgatg
    12061 ctggtcatgt ctttcctgct gattaacgtc tttaacattc gcggcggtgg gtatatgtac
    12121 ttcattacct acgtcttaca aggcagtacg ggctacacgt cgctgttctt caccatggtc
    12181 accttcgcct ccattatcgg ctcggtgatt gtcagcccgt taacgcggcg tttcgatacc
    12241 gtcaaaattt actactacac caaccagctc ctcgctgcgc tggcggtgtt gatgtggttc
    12301 ctgccctccg gcccggctta tcaaacgctg tggctggcgg tgatcctcgg taatggcgta
    12361 attcttggct tcacattgcc actgcacttc tcattgatgg cctttgccga tgactacggc
    12421 gagtggaaaa cccgcgtacg ttcttccggc atgaacttcg ccttcaatct gtttttcatc
    12481 aagctggcct gggcctccag cgccgggatc atcagcctgc tgtttatttt tgtcgcctac
    12541 cagcctggcg tggaaaacca gaccgccagt tcgcttggcg ggatcgcggc gatggaaaca
    12601 ttactgcctg cgctattcca cctgctgctg gcaggggcga tccgcttttg caaactcaat
    12661 aatcctatga tgtcacgcat agctaccgac ctgcgtcagc gtcatgtaca gccttaagga
    12721 gcacaagatg aacatttcgg aagtcgatct gcataaactg acggtcagcg atccgttcct
    12781 cggtcagtac caacaactgg tccgcgacgt ggtgattcct tatcagtggg atgctttgaa
    12841 cgatcgtatc ccagaagcgg aacccagcca tgcgattgaa aactttcgca ttgccgccgg
    12901 acttcaggag ggtgaatttt acgggatggt gtttcaggac agcgacgtcg ccaaatggct
    12961 ggaagcggta gcctggtcgc tgtgccagaa gccggacgcc gaactggaaa aaaccgccga
    13021 cgaggtaatc gaactgatcg cctccgccca atgcgaagac ggctatctca atacttactt
    13081 tacggtaaaa gcacccgaag aacgctggag caatctggcg gagtgtcatg aactttactg
    13141 cgcaggtcat ctgattgaag ccggagtcgc cttcttccag gccacgggca agcggcgctt
    13201 gctggaagtg gtttgccgcc tggccgatca tatcgacagc gtatttggtc cagatgaaag
    13261 taagttacac ggttatcctg gtcacccgga aattgaactg gcactaatgc gcctgtatga
    13321 agtgactgaa gagccgcgct acctggcact gacgaactat tttgtcgaac agcgtggtgc
    13381 gcaaccgcac tattacgacc aggaatatga aaagcgcggg cagacatcgc actggcacac
    13441 ctacggcccg gcgtggatgg tgaaagacaa agcctacagc caggcacatt tgccccttgc
    13501 gcaacagcaa accgccatcg gtcacgcggt acgttttgtc tatctgatga ccggcgtcgc
    13561 gcatctcgcg cgtttaagtc acgatgacag caagcgtcag gactgcctgc ggctgtggaa
    13621 caatatggcc cagcgtcagt tatatattac cggcggcatt ggctcacaga gcagcggcga
    13681 agcgttcagc agcgattacg atctgccgaa tgacacggtt tacgccgaaa gttgtgcttc
    13741 catcggcctg atgatgttcg cccgacgaat gctggaaatg gaaggcgaca gtcaatatgc
    13801 cgatgtgatg gagcgcgcgc tgtacaacac tgtgctcggt ggcatggcac tggatggcaa
    13861 acatttcttc tatgtgaatc cgctggaagt acatccaaaa tcgctgaaat tcaaccatat
    13921 ctacgatcac gttaaaccga tccgccagcg ttggtttggc tgcgcttgtt gtccgccaaa
    13981 tatctcccgc gtgctgacct cgattggtca ttatctctac acgccgcgtg aagatgcgtt
    14041 gtatatcaac atctacgcag gaaacagcat ggaagtgccg gtagaaaatg gcacgctgcg
    14101 cctgcgggtt agcgggaact atccgtggca ggagcaggtg acgattgtgg tcgaatcgcc
    14161 ccagccggta cgtcatacgc tggctttacg tctgccggac tggtgcacac agccgcagat
    14221 catattgaat ggggaagagg tcgagcagga tattcgtaaa gggtatttgc acattacccg
    14281 cgaatggcag gagggcgata cgctgaatct gactttgccg atgccggtac gccgcgttta
    14341 cggtaacccg ctggtgcgtc acgtcgccgg aaaagtggcg attcagcgcg gcccgctggt
    14401 gtattgcctg gaacaggccg acaacggcga gtcactgcat aatctgtggc tgcccaccga
    14461 tgcgccattt acgacatttg aaggcaaggg attgtttagc cataagatct taatccaggc
    14521 accgggttac cggtatgaac agagcaatcc agagcagcaa ccgctgtggc attacgacag
    14581 cgcgccagcc aaacgccaga cgcaaactct gacctttatc ccgtggttta gctgggccaa
    14641 ccggggtgaa ggcgaaatgc ggatctgggt gaatgaggaa aagcattgcc atccgtaggt
    14701 tggataaggc actcgcgccg catccaacgt aaggagcaca ttgcctgatg cgacgcttaa
    14761 cacgtcttat caggcataca agtcggtgcg agagcgtagg ccagccgcac cgacatccac
    14821 cggtgaatta aaaacgccag ctcacgccgg tgacaaaggt aaaactctca ttacgatcaa
    14881 tcatcgggct gtcggtaatc tcttccggca gctcgctgta tcctgcgctc gccatcaata
    14941 cgacgtgctc tcctatcggg tattttgccg tcaggctgac atagggcacc caggcatcct
    15001 gcgcggaata acttgccaga ccgctacgac gggactcact ctctgaaatg ccatagtaat
    15061 agtcactgaa attctcgtca taatagagaa cgcccagcgc aggtgtcagc gacagacgac
    15121 ctatctgcat tttgtggaat accgatagct cccccaccca gccgttgctg ttatccagaa
    15181 cgtccgcagc tgcagaggct ttcacggttc cccaacgctc gtggtgatac caggcaaccc
    15241 ccgccatagc cgtactatca cgcttatcaa gctgttgcat ggcataatcg tcattatcgg
    15301 cagggtcaaa ttccagcggc atccaggatg cggtcaggct aagttcattt ttttcacttt
    15361 gcgacagaat aaaacctaac gtggtctgac gaacataaaa agaatcacct tcataactaa
    15421 tcaggggaat tgcctttgta ttttcattat aaccgcgata aggcgattca ttataagccg
    15481 caccggcacc aatggataat tcagaagcag ttgcgcttgc cataaaaggc aacgcaaata
    15541 acgccactat attgcggtta attaacatgt caggtattcc attttaaaat aagataaaaa
    15601 agagtcggca tagtaatgct tacaatttct ccggtgcaag attcatattt atatagtaat
    15661 atataaaatt atatataatt gggctgttgc gatgacaaaa ttacaactta agtaccggga
    15721 gttaaaaatt atctcggtaa tcgctgccag tgaaaatatc agccatgccg cgactgtact
    15781 tggcatcgca caggccaacg tcagcaaata ccttgctgat tttgaatcaa aagtgggttt
    15841 aaaagttttt gaccgaacca cccgacaact gatgctcaca ccttttggca ccgcgctatt
    15901 accgtacatc aacgacatgc tggacagaaa cgagcagctt aataatttta ttgcagatta
    15961 taagcatgag aaacgtggtc gggtcaccat ctacgcccca accggcataa ttacctattt
    16021 atccaaacat gtaatcgata aaattaaaga tatcagtgat atcaccttat cgctaaaaac
    16081 ctgcaatctt gagcgtaatg ccttttatga aggcgtcgaa tttcctgatg attgtgatgt
    16141 tttaatcagc tatgccccac caaaagatga atcgctggtt gccagtttta ttacccaata
    16201 tgccgtaacc gcttatgcca gccagcgcta tcttgagaaa catcccatta gccgtcctga
    16261 cgaactcgaa catcactcct gtattttgat cgactcgatg atgatcgacg acgcgaatat
    16321 ctggcgcttc aacgtcgccg ggagtaaaga ggtgcgggat tatcgcgtga agggaaatta
    16381 cgtttgcgac aacacgcaat ctgcgctgga gctggcacga aatcacctgg ggattgtgtt
    16441 tgcgccagac aaaagtgtgc agagcgacct gcaagacggc acgctggtgc cctgctttca
    16501 gcacccgtat gaatggtggc tggatctggt ggctatcttc cgtaaacggg aataccagcc
    16561 ctggcgggtc cagtatgtgc tggatgaaat gctgcgtgaa attcgccacc agcttgctca
    16621 gtcgcagcaa ctgcggcctg aacaagcggc ggaaagcgaa gattaatgat cgagataaag
    16681 gtaatgcacc cagctggtca tgcgtaataa cactttgcgc atcaccgaga catggctgaa
    16741 atgatcagag tagaccgcca cctgggcgta aataccgtct ggtaaagcgc tcaaatcagt
    16801 gtgctcattc agttcaatgg tcgcgataac gccatctgaa cccggcgctg tgtttaacgt
    16861 ctgtaaggtg ccggtcgact gataagctcc gccgggaaca gctggactaa tggctgccag
    16921 cttaccgctg aacactttgc ctggcagagc gttaaatacc acttccgcat catcgccggg
    16981 agccaggcgc agcaaggagt tctgacggaa ctgcgccacg atttgtcgtt tctgatcggg
    17041 tataaacacc atcaccggac gtagcggcag cgacgcggca taggtacccg gacgaatcag
    17101 cacttgggtc acataaccat cgctcggcgc acgcactatc gtctgctcaa ggttatattt
    17161 tgcttccgcg agctgcgctt tcaggctggc gatttgagaa tgttcaccca acaccaggct
    17221 atccagctgg ctctggatct gtttttgttc cgccgccgat gactttacgg aggcttcctg
    17281 cgccagataa ttttgccgtg ccacatcaat atcgcgttct gaaaacgggt ttactttcgc
    17341 ctggctgccg cgtgcgtaac gctggtactc tttagcgaat ttatcccgcg tggcctttgc
    17401 ctgctgagta ttcgccgcca tctcatctaa ctctgcgccc aacgcccgct gtttatgttc
    17461 tgcggtaacg atatccgcca tcagccgatc cacccgcgcc tgataacgcg tcgggtccag
    17521 tcgaaatagc acctcacctt ttttaattag cgtatttttc ttgtccgtca cttcgatcac
    17581 cacgcctgtc acctgcggga caacaggaat agaaataacc gctttttgcg ctttaaaggt
    17641 atacggatgg ttatagttca ttaacagaat taaaccgctg acaataaata tgccccccag
    17701 ggccgctgtg ggaatggtcc atttatttac aggaattttg aagatcttaa acattgccca
    17761 tgcgaaagcc acataggtca aaataatcag taaatccata agtattactc ggcagaagag
    17821 gatttgatgt cggcgagctg tttctccagc ccggcaatgc gctgttgcag ttgcacaacg
    17881 gaggagtcat ggttttgcat tccccatccc cgctccgggc ggtaaagcgt ggcccaaatc
    17941 cacaaaaatg gccagataac atgcagcgta aaaagactca cccaaccagc aacatgaatg
    18001 gcgtcggcat gaggatgatt acgtttttta gcaatcaggt agggaatatc atgtaaaata
    18061 ataatcccat agaatatcac cagaaataca aaaataagca ctcccagtgc aaaatagtcc
    18121 aggaacatat ttccctcaga gaatataaaa aagaacaatt aacgcatatt atgaaatgcc
    18181 atgatgcagt gcaattttct tatttatata acgacatatt gttttttata tacttcctga
    18241 agtgtgctgc ctggcttttt aacaggtcga ggcttatcat attgaaataa tggaactatt
    18301 ggcaaaattt atgattaaaa accgtaaaac atccacaatg cagattaagc gtattaaacc
    18361 ttcaataatc taccgtttat tgctgattgt cctcggaata ccaatgatga tttacggtct
    18421 ggtttgcccg ctcaccatcg aaacgcgaga ttaaactcct ggcaaaacac gccccagaaa
    18481 cgcacttctg ttggcgtagt ctgggttatt gcgcacgtag gtttaaaacg taaattgtaa
    18541 caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc tacggtcagg
    18601 tccccgtagg catgataaga cgcgtaagcg tcgcatcagg caattaatac ccgatgcgac
    18661 cagcttctta catcagaaca gccccaacgg tttatccgag tagctcacca gcaggcactt
    18721 ggtttgctgg taatgctcaa gcatcatctt gtgggtttcg cgtccaatgc ctgattgttt
    18781 gtagccacca aacgccgcat gtgccgggta agcgtgataa cagttggtcc acacgcgccc
    18841 tgcctgtatg ccgcgcccca tcttataggc aagattaccg ttgcggctcc agacgcccgc
    18901 gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg ttttgaaggt
    18961 ggtcaccgcc agcaccgggc caaaaatctc ttcctggaac acccgcatat tgttctgacc
    19021 aaacagaatc gtcggttcaa ggtagtagcc gtctttcagt tcgccttcca gcagcttgcg
    19081 ccgcccgccg gtgagcacgt ctgcgccctc ttttttaccg atatcaatgt agttgaggat
    19141 ggtttccagt tgcccgtgag aaacctgcgc ccccatttgc gtcacgctgt cgagtgggtt
    19201 accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt cgtagattga
    19261 ctcctgcact aaggcacgac tcggacaggt gcaaacttcg ccctggttaa aggcaaacag
    19321 tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca catcagcaaa
    19381 gaagatattt ggcgacttac cgcccagctc cagcgtcacc ggaataatgt tttgcgttgc
    19441 gtattgcata atttgttggc ccacttccgt tgagccggta aacgccactt tggcgatgcg
    19501 tttcgaggtc gccagatatt cgccaatttc cccacctgcg ccattgacca cgttcaccac
    19561 gcccggcggc agtaaatcac cgacaatttc cattagcagc agtacagaaa gcggggtaag
    19621 acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca ttttccagct
    19681 cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta acggttcatg
    19741 gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct gcgcccgaat
    19801 acacgaggcg aaatagcgga aatggtcaat cgccagcggc acgtcggcag cactggtttc
    19861 gcgaatgggt ttgccgttat cccaggtttc agctgtcgct aacagctcga ggttttgttc
    19921 cattcgatcg gcaatcttaa acagaattgc cgcgcgatcc tgcaccgagg tatatgccca
    19981 tttatctttc actttatgcg cggcatccag cgccagatcg atgtctcgtt tgcccgaaga
    20041 cgccacttcg cacagcagct gcccggtcac cggcgtcagg ttctggtaat actcaccatc
    20101 ggcaggggct acccattcgc cgccaataaa gttgtcatag cggtttttta acttgagggg
    20161 gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca tgatcgtctc
    20221 cttgcggtgt gaggtataac aagggtagac gtgactggcg aaatcttcgc cagtcggtaa
    20281 cagctttacg acagctatca cgaatttacg ggcaaggatt acagagatag ttgaaaaaat
    20341 ggctgacaat ttgccagccg ttgtggaaat gatgattaca ttgcggcgcg ataaatcgcc
    20401 acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cacaggcatc tttcagggca
    20461 ttagtcgcca gtaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
    20521 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttccgcg
    20581 tcgtttttac ctgtcacatt cacgcccatt gcagcggcgc agtcacgcag acgtgcagcg
    20641 gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca tacaccgtgt
    20701 ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc cagagaagca
    20761 ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg cgctttcgca
    20821 ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
    20881 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
    20941 aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccgatcat cagcgaggag
    21001 tcattgacag aaagcagcgg agtgacatgt ttatcaacaa tcgccatttt gatatgacgc
    21061 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtattg
    21121 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata
    21181 tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgcgg agaaccaccg
    21241 cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc tgcggcgacg
    21301 ttttccgtgg tggggttagg ttgggtacca tcataaataa tgctaaaaat attgcgttct
    21361 tccagtgctt tttgcacatc gcccgccata cctaatttcg ttaacatact gtcagtgaca
    21421 atcaatgtac ggcgaaaccc atattctgcc atcatattca ttgcatcagc caatgaatca
    21481 gcgccgatga cattcacaga aggaataaaa aaggttgaag ctgccataac acactcctga
    21541 aagtgtaaaa aagggcgggc agcttactga ggattttcat tcgaaaatat gatcagtatt
    21601 attgtttagc aaaattaatc taataaaaag cacattgtat tcattaaaca atg
//
LOCUS       DAZGCQ010000086        21130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000086 DAZGCQ010000000
VERSION     DAZGCQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.086"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..120
                     /locus_tag="ACHK07_003567"
     CDS             <1..120
                     /locus_tag="ACHK07_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO8103851.1"
                     /translation="AEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            139..783
                     /locus_tag="ACHK07_003568"
     CDS             139..783
                     /locus_tag="ACHK07_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO8103852.1"
                     /translation="MSRIITSTVYTLNELSATAREKARDGYRQHHADSNWYENVYEDF
                     RKVCDIFGIDLRQRVFQLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARIIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQIMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDATVDEALLINEYTFTEAGLRAG"
     gene            833..1201
                     /locus_tag="ACHK07_003569"
     CDS             833..1201
                     /locus_tag="ACHK07_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8103853.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYIAIYPTPGTKT"
     gene            1291..1668
                     /locus_tag="ACHK07_003570"
     CDS             1291..1668
                     /locus_tag="ACHK07_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8103854.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     NERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            1665..2153
                     /locus_tag="ACHK07_003571"
     CDS             1665..2153
                     /locus_tag="ACHK07_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8103855.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            2165..2362
                     /locus_tag="ACHK07_003572"
     CDS             2165..2362
                     /locus_tag="ACHK07_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8103856.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2447..3313
                     /locus_tag="ACHK07_003573"
     CDS             2447..3313
                     /locus_tag="ACHK07_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8103857.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAIKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKENYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            3385..3648
                     /locus_tag="ACHK07_003574"
     CDS             3385..3648
                     /locus_tag="ACHK07_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8103858.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            3645..3971
                     /locus_tag="ACHK07_003575"
     CDS             3645..3971
                     /locus_tag="ACHK07_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8103859.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(4386..4922)
                     /gene="yghD"
                     /locus_tag="ACHK07_003576"
     CDS             complement(4386..4922)
                     /gene="yghD"
                     /locus_tag="ACHK07_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO8103860.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIIAARKSATTAPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYVLRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(4924..6102)
                     /gene="gspL"
                     /locus_tag="ACHK07_003577"
     CDS             complement(4924..6102)
                     /gene="gspL"
                     /locus_tag="ACHK07_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8103861.1"
                     /translation="MSSILEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGSWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRDQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(6099..7076)
                     /gene="gspK"
                     /locus_tag="ACHK07_003578"
     CDS             complement(6099..7076)
                     /gene="gspK"
                     /locus_tag="ACHK07_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8103862.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(7073..7678)
                     /gene="gspJ"
                     /locus_tag="ACHK07_003579"
     CDS             complement(7073..7678)
                     /gene="gspJ"
                     /locus_tag="ACHK07_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8103863.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVTGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(7675..8046)
                     /gene="gspI"
                     /locus_tag="ACHK07_003580"
     CDS             complement(7675..8046)
                     /gene="gspI"
                     /locus_tag="ACHK07_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8103864.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(8043..8606)
                     /gene="gspH"
                     /locus_tag="ACHK07_003581"
     CDS             complement(8043..8606)
                     /gene="gspH"
                     /locus_tag="ACHK07_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8103865.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(8610..9065)
                     /gene="gspG"
                     /locus_tag="ACHK07_003582"
     CDS             complement(8610..9065)
                     /gene="gspG"
                     /locus_tag="ACHK07_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8103866.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(9082..10305)
                     /gene="gspF"
                     /locus_tag="ACHK07_003583"
     CDS             complement(9082..10305)
                     /gene="gspF"
                     /locus_tag="ACHK07_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8103867.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPMHIEA
                     RMNASAGGMLQRRRHAHRRVASADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPVMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(10305..11798)
                     /gene="gspE"
                     /locus_tag="ACHK07_003584"
     CDS             complement(10305..11798)
                     /gene="gspE"
                     /locus_tag="ACHK07_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8103868.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDAHTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(11798..13858)
                     /gene="gspD"
                     /locus_tag="ACHK07_003585"
     CDS             complement(11798..13858)
                     /gene="gspD"
                     /locus_tag="ACHK07_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO8103869.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(13888..14847)
                     /gene="gspC"
                     /locus_tag="ACHK07_003586"
     CDS             complement(13888..14847)
                     /gene="gspC"
                     /locus_tag="ACHK07_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8103870.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(14865..15275)
                     /gene="gspS2"
                     /locus_tag="ACHK07_003587"
     CDS             complement(14865..15275)
                     /gene="gspS2"
                     /locus_tag="ACHK07_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO8103871.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQITQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(15341..16150)
                     /gene="pppA"
                     /locus_tag="ACHK07_003588"
     CDS             complement(15341..16150)
                     /gene="pppA"
                     /locus_tag="ACHK07_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO8103872.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(16477..21039)
                     /gene="sslE"
                     /locus_tag="ACHK07_003589"
     CDS             complement(16477..21039)
                     /gene="sslE"
                     /locus_tag="ACHK07_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO8103873.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRV
                     TGATCNGESSDGFTFKPGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQE
                     LAGSDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKL
                     VNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSE
                     GRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTI
                     ALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNG
                     ATLGEGEQVVNLPNEFIEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQ
                     GVINKLWGVDEDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKN
                     YWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGN
                     PHYNSILRCPNGYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASM
                     TVGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFE
                     YVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASG
                     FVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDT
                     TKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRI
                     LDAFPGLEECKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAA
                     DLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDEL
                     GFKTFTEFLNCYTNDAYTDGTQCSADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYME
                     KPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGL
                     WAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKF
                     KVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESASFVY
                     TTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRF
                     TNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGAT
                     EVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKE
                     WAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCA
                     ESNGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGVSEMNFEGGVSQSAYETL
                     AALGLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         4877 a         5910 c         5319 g         5024 t
ORIGIN      
        1 gctgaggcgt ataccggtcg tgaagtgcag gatattcccg gtgtgctggc cgtgtttgct
       61 gaacgccgta aggacagttt tggcccgtat gtccggctga tgagcgtcac cctgaactga
      121 atcaggacgg gcattcagat gagcagaatt atcacctcca ccgtgtatac actgaatgag
      181 ctatccgcca ccgcacggga aaaagcccgc gacgggtacc gtcagcatca tgctgacagc
      241 aactggtatg aaaacgtgta tgaagacttc aggaaggtct gtgacatttt cgggattgac
      301 ctccgccagc gcgttttcca gctcagcaac ggcaggttta tggaagagcc gtgcatctgg
      361 ttcagcggat tctgcagcca gggggacgga gcctgttttg aaggacgctg gcactggcag
      421 tcagcaacag ccaggataat ccgggagtat gccccgcagg accgtgagct tcatcgtatc
      481 gcggatgccc tgcaggcggt gcagaagcgt aacttctggc aactgcaggc tgaaatccac
      541 caccggggac gctactgcca tccgtacagc atggatatca ccgtcacccg gaacagtccg
      601 actggtcaga taatgacaac cgatgcggaa gccgctgtca gcgaagccct gcgcgacctg
      661 gccttctggc tgtaccgtca actggaaaac gagtacgact ggctgacctc agatgccact
      721 gtggatgagg cccttctcat caacgaatac acctttaccg aagccggtct gcgcgccggc
      781 tgacccgtca tcgttaaact atcattcacc gattttctgt gaggatttta tcgtgtcaga
      841 cacactctcc gggacaacgc atcctgacga caatcacgac cgcccctggt ggggactccc
      901 ctgcacagtg acgccctgtt tcggggcacg tctggtgcag gagggtaacc ggttgcatta
      961 ccttgcagac cgcgccgaca tcagaggccg gttcagcgat gcggatgcat accatctgga
     1021 ccaagccttt ccgctgctga tgaaacaact ggaactcatg ctcaccagcg gtgaactgaa
     1081 tccccgccat cagcataccg tcacgctgta tgcaaaaggg ctgacctgcg atgccgatac
     1141 cctgggcagt tgtggttacg tgtatatcgc catttatccg acacccggaa cgaaaacata
     1201 aaccaccaga ataaacctct gctactgccc ggtgcaccac agcaccattc tcctcaaaat
     1261 ttaatcgctc atatcaggaa aatcttcatt atgaaaacat tacccgacac acacgtacgg
     1321 gcggcatcgc gctgcccgtc tccggttgaa atctggcaga cactgctcac ccgactgctg
     1381 gaccagcact acggcctcac gctgaatgac acaccgttcg ccaatgaacg tgtgattgag
     1441 cagcatattg aggcaggcat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac
     1501 gcgctggtac gaactgacca gccaggattc agcgcctgta cccgctctca gttaataaac
     1561 agcatcgata tcctccgggc tcgccgggca acaggcctga tgacccgcga caattacaga
     1621 atggtaaata acattaccca gggtaagcat ccggaggcgc aacaatgaaa ctggccctga
     1681 cactggaagc cgacagcatt aacgtgcagg cactgaacat ggggcgcatt gtcgttgacg
     1741 tcgatggcat tgagctcgct gaactgatta acaaagtcgc tgaaaacggt tattcactcc
     1801 gtgtggtgga cgactccgac caacagtcaa cctgcacacc accaccgtta acaaccctta
     1861 ccggcatacg ctgcagcacc gcacatatca cggaaacaga caacgcctgg ttgtactcgc
     1921 tgtcacacca gaccagtgac ttcggcgaat cagaatggat tcattttaca ggtagcggat
     1981 atctgttacg taccgatgcg tggtcatacc cggttctgcg gcttaaacgc ctggggctgt
     2041 caaaaatgtt ccgccgtctg gttgtcacac ttatccggcg ttatggcgtc agtctcattc
     2101 atctggatgc cagcgctgaa tgcctgccgg gtttacccac tttcgactgg taaccagaaa
     2161 caacatgaaa tcattaacca ctgaaacagc gctggatatc ctcattgcgt ggctgcagga
     2221 caatatcgac tgcgaatcgg gaattatctt tgacaacgat gaggataaaa cggattcggc
     2281 agcactgttg ccctgtatcg aacaggcccg ggaggatgtc cgtactcttc gtcatctgca
     2341 gcttctgcac cagaaccggt aaatatcact caccagactt cattccactg acgccagcct
     2401 gaacagggct ggcgttttca tttatctgca aaaaggaata tcgattatgt caggtatcac
     2461 aatctcccgt cctgaagtgg tcaacgggca tacggacgtt atctgttcca cctcaatcgg
     2521 acacattctg gctgtacgaa agaccacact gatgcaaatc gacacactta tccggcaact
     2581 gacagaaatc tcagcaatga cagaaagtat tggaggtaaa accgccccgg actgggccat
     2641 aaaacagaat tttcgctgcg gttgctggct gatggagaaa cccgaaacag caatgaaagc
     2701 cattacccgg aatcttgacc gcgaaatctg gcgtgacctg atgcaacgtt ccgggatgct
     2761 gtcattaatg gatgcacagg cacgtgatac atggtaccgg tcactggaat acgataattt
     2821 tccggaaatc agtgcggcaa acattctgag cacatttgaa caactgcacc agaataagga
     2881 tgaggtgttt gagcgagggg tgatcaacct cttcagggga ctgagctgga attacaaaac
     2941 caactcaccc tgtaaatttg gcagtaaaat tatcgtcaac aacctggtga ggtgggaccg
     3001 gtgggggttt cattttatca ccgggcaaca ggcagatcga cttgccgatc tggaaagaat
     3061 gctacacctg ttcagcggca aaccgatccc cgacaaccgg gaaaacatca cccttcgtct
     3121 ggatgatcat atccagtctg ttcagggtaa agagaactat gaagacgaga tgttcagtat
     3181 taaatacttt aagaaaggct cagtacacat cacgttcagg aaaccagaac tggttgacag
     3241 gcttaatgat attattgcca gacactaccc ggaagccctg gcaagcacag tgaacaaaag
     3301 ccgcaaggca taaaagaggt attttacaat tcgcgaatat taactattat taattgatag
     3361 caatcaatcg gcgagaaaat caccatgcct accagtgtat ccctcagtcc ttactttgaa
     3421 acgtttatcc gcgaacaaat agaaagtggt cggtataaca atacaagtga agtcattcgt
     3481 gccggacttc gtgctctgga agaacgggaa cagcagataa agcttgaatc actccagagc
     3541 gccgttaccg caggcatcaa cagcggtgaa agtaaaagtg ctgaagaggt attcgggcga
     3601 ctgacgcaca aatacaaaaa gatggcagaa ggtgaacagc caatatgaaa ctgactgttt
     3661 caccattagc agaacacgat cttgaagcta taggtgactg gatcgctcag gataatcctg
     3721 tccgtgcaat cagttttacc gaagagcttt accaacaatg tctgttgata gccgaatcgc
     3781 cagtactcta cagagaaaga cccgaattag gggctgggat cagaggttgt tcgtatggtc
     3841 gttaccttct gctgttcagg gtactggata ctgaagttcg tatcgaacgt atcgttcatg
     3901 gttcccggga tatcaacagg gtacttgcag agccagacga caaaccatca gatcatttct
     3961 ggcagccata aacaaaatct gtgcgttaac gctccctaac acaggtagcc aggtatttta
     4021 taaatctctg tattttgttg ttacttcgat tccgcaattc agcatatcga accagacaaa
     4081 atgattacag caaagcacaa aaaagaggga ttatagcgtc aaaagaattc gaattgcaca
     4141 tgggggcctc attggtggcc tcgaaaacaa caaagacaaa acaatcaata caaaacaatt
     4201 ggttatataa ccagtttcat tccgagtccg ggcaccacta attcttaaga acccgcccac
     4261 aaggcgggtt tttgcttttg gatctgacaa taaccttcac gaaaaaaatt agcttataaa
     4321 gtctggggga attactctcg ccacgttaac gagagtaatt ttattgatat taatctcctg
     4381 aaactttacc ctcgtccaaa ctccagccgc tgcacattca ccatcccagt cttctcagca
     4441 gcactgacat caatttgtgt tacccgcagc acatattttt catccagtgc gtttaaccat
     4501 ttcagaaggt cattaaacac cacaggttct atccagacct gaatattctc cccacgctcg
     4561 gctatccgct tgatgaccac cgagtgcgcg gaagcactgt cactgatgac ccgcgatacc
     4621 tgcgcaggtg ctgtcgtggc ggattttcgc gctgcaataa tatccggcgc ggcgctcctc
     4681 agtcgggtgt tcatcgccac cagttgctgc aacatcgtct cctgttgttc tatccgttcg
     4741 ctcaacggct gccagatgag gacgtaatat ccgacgctaa acaggaacac caccgctgcc
     4801 agtaacatgc ccttttcacg cggagaacgc cccgccaggt gttgtgccag ccagtgttcg
     4861 ccacggctta actggcgttc acgccattgc tgaaaatagt gaataaattt atcgcgtaac
     4921 atgttatttc ctccgcaacg ttacgccgcc ggaaaccgca tcaccctctt tctgtaacgc
     4981 gtcctgttgc acaacataat cggttgccag tgcactacgc agtttgtcga aactggcaaa
     5041 gtttgctgcc cgcaactgga ggtgaagcgt ctggcgtttt tgatcaaagg tgaaaccacg
     5101 catctcgatg tcggaaagtg acgctgattt cagggtgctg gctatcgctg acaactcggc
     5161 gagcagccgg gtatcgtcgg cctgtgggcg atattttttc agcgccatcg tcacctgaga
     5221 gcgtaaattc acaatccgct tctgctccgg gaacaacgtt aagaactgtt tctccgcctg
     5281 gtcgcggctt tgcgccacct gttcgctgac gctccataac gtcacgcccc gttccactgc
     5341 cagcgcaacc agaatcagca atatcggcag aatcatcacc cgccagcgcg cccactgttt
     5401 tcggtagctg acgcgaggct gccacgaccc tgttagcagg ttcccttccg gttcgccata
     5461 agtggtaatg gcgggcagag ccgtaacggt caggcgttca gcatctgcgc cagcccatgc
     5521 tgatagctct tccggtgcaa tgccgactac ggttagcgaa agcggtaaat cctgctcatt
     5581 tagctgggcg cggaacatga ccggagccag cgcccgcccg gcgctccatc cccggcattc
     5641 atcgatgcgg cagataaccc gttgcgcatc gccagccata tagccacaag gtatggacat
     5701 ccagtctggc gcgacggtgg cgcgggtgat gccgtttgcc tgcaaccact gcgcaatgtt
     5761 gcgcatatgc tgttggtgaa tcactgccac ggttgccagt tgctggtcga ttttcaacgg
     5821 ggcgaaatgc agttcatcga tatcctggtt cagctcttct tccagtaagg cgggcagaat
     5881 agtcggtatc tgcttgcgtg ggacatcagg cagttcaacc tgccagacgc tgatccattc
     5941 gccgggaatg tagagtcgaa tcgcatcagt ttgcagccat tgctggagac gttcgtcagc
     6001 aacgtcaggc cagataccgt gttccacgtc gggtgtacgg cgctgccagc ggatggtgtc
     6061 ggaggcgcaa agcgggaaaa aaatctcaag gatggaactc actcactttc tcctgtctga
     6121 tgccagagaa cagaaaagtg ttgtgggccc atgcggacaa ttaacgagtt catcgtcagt
     6181 tcaatctcat tcacggtgat atctgaacgc agccagaagt aattgctgtc cacgctcagg
     6241 acggttttta gctgtttttt agtacgctca tcgacgtcag caagtagcgg ctgtgcaaga
     6301 aactgatcga catcttccca gcccttcgcc ggacgttgtt gtaataacgc ccgcgcctga
     6361 acagggctta accacgggtc aaacagcgcc tcaagaatca cactttgcgt gacgtctaaa
     6421 gtattgatgt tgatttgctg gcgggtcatc ggcagcgcac agaccagcgg tttcagtttt
     6481 tgataaagcc cggcgtccat tccctgcacc acgcgcatct cgctgatatc ggccagcggt
     6541 tgattggcgg cgtagaaagg caccgagcgg gcgagatatt cgctgtcttc acggcccaga
     6601 cgcgtctgca cgctgcggtc ttcatcaata aactcccaca ggctttcggc tatcagttcg
     6661 gcccgataag caggcacatc gaggcgcgag atcagggcaa tcagttgttg taacgcgagc
     6721 ggacgcgacg ccgtcgtcgg ctgagcgagg gcattcaggt taaagcacgc ctgtgcgtca
     6781 cgcagagtga cggcgatttg cccctgcggc agtgggaaaa aacgagggcc ggaagcccac
     6841 ggctgtgcca gatgcacgcg cttttcattt ttcaggctca gactgagtgc actcagcgcc
     6901 aggctttctg cactggcgct gtaccacagc gcctgctggt actcctgctg gctgcgggtt
     6961 cgcccaagtt gttgctgcat ccgcccggaa agcgtgatgg ttaccagcat cataaccgcc
     7021 agcaatacca gcaccacaac cagtgccatt ccgcgttttg gtggtgaggt gatcatgata
     7081 attgcggccc gcgtaacaac cagatgcgtt caatctcgcc ccattgcggc gaatgcaggg
     7141 tcatgcgcac cgccacaggg atcgcctgta ctgacgacca gttctcctgc cagcgcgtac
     7201 catcatagaa ctgcaagcgg agcgaatccg ccggaatcaa cttttgcatt gtcggcttca
     7261 cgctgcctgc tgcgtcggtc agcggccagg ctaaccgttc gagatagccg tcatgaatgc
     7321 ggtaaccgac ggtgagcaga ttgctgcgtg gcagacgcat caacggatta accacgccgc
     7381 cacgcacaaa gcgcatccct tcactctcag atgccagcac gccagcgcca gccagtaacg
     7441 ctggttctcg ctggccctga tcgcctctta ccggacgcgg catcatttgg gtcagatcgt
     7501 gggtcagaaa actcatcgtt tgctgcatga ggtttagttt ttgatcgtgt ccggtgacgg
     7561 cgctattcac gcgcgtgacg ccgttagtca cctgctgcgc catcagtgcc agcgaggcaa
     7621 aaatggcaat tgccaccagc atttccagta acgtgaaacc agcgcgagcc cttctcactg
     7681 ttggcctccc acggcgctaa accaggcgcg tcgtgactga attaccgacg aaaagtcttc
     7741 gtgaaggctg acttcaatat ccaccgcctg gagcagcgcg ttgcctgtat tctgcggtgt
     7801 ggttcgccag taccagcggc ttccagccat atcgctctcg ccctgatgcc tgaccgcccg
     7861 ttgttcacgg gtcatcaggt agagcagcgc ggtctggttt tcagccaccc agcctgctac
     7921 cgttttctcc tcaagaacgt gctgattact cagcgcaccg ctggcaatct gtaacaccgc
     7981 cgtggcagcc agcgcaaaaa tcgccagtgc gagcatcact tccagcaagg taaatccgcg
     8041 cttcatggca tcctctcatc acattgactg agggataacg cgccatcgtg tgccagtttt
     8101 accgcccaac atgcgttttg cgccgctgag tagaagcgca gcgtaaacgg cgtggcgggt
     8161 tcaaaaggcg aaaaacggat ctgcggcgtc ttctttttcg cctctttttg caactccagt
     8221 tcaatatcgt gcagcgtcag gcgacgacgt tgcagctcca gcgcatattc cttctgccag
     8281 atatcacttc cgggttgtaa cagcatctgc acctgtttcg gcaccgtaac ctgtgccgat
     8341 aaccgggtcg cagaaacggg caaccactgc ccctgacgac gttgcataaa ctggtatccc
     8401 ggcgggtcga ttaacacacc aagtgtttgc ccttcgatca ctgccctgtc cttaaactgc
     8461 gcaaagcgag tcagaaaatc ctgcgccgct tttttcgcgg gcgattcaga atcggtcgca
     8521 aaagtctgta ccacgcccgc actggcaagg ccgataagga aaatcaccag catgatttcc
     8581 agaagtgtga atccgcgttc aggcactgct tactgaaact cctgcaaatt ccagttaccg
     8641 atatctgcgc cagcgccttc cccgttttcc tgaccatccg cccccagggt ataaacatca
     8701 aacagccctt tttcacccgg gctgagatac tgataatcat tgccccacgg atcctttggc
     8761 agtcgtttaa tgtatccacc agtacggtag ttgcgggcgt ccgccatatt ggctggctgc
     8821 tggatcagcg cctcaagccc ctgctcggta gttggataac gcccgttatc cagtcggtac
     8881 atatccagtg cgttttccag cgccacgata tcgctgatgg ctttttgccg atccgctttc
     8941 tctttattgc ccaacaggtt aggcaccacc agacttgcca ggacgccaag aataacaatc
     9001 accaccatca cttccagcag ggtaaaacct gcccgtggtt tttgtgtgcg ggataacgaa
     9061 ttcatgtgat aactccgtaa attacattcc aaccatattg ttcagttgca gcatcggctc
     9121 gaggatggcg atgacgataa acagcaccac gcccgccatc atcaccacca gcgccggttc
     9181 aaacagccct aacgccagcc ccacctgggt atcaaattcc cgctcctggt tgacagcggc
     9241 ctgctcaagc atggtttcca gttcgccgct ctgttcgccg gaggcgatca tgtacagcat
     9301 catcgggggg aacaggcgca actccgccag cgcggcgcgc agactgcttc cttcgcggac
     9361 gcgatctgcc gccagcagca gttgttgctc gacatagcga tttgccgaca cagcggcagc
     9421 ggtctgaatg ccttccagca gcggaacact gctggcggtg aggatgctta atgtgcggga
     9481 aaaccgcgcc gtattcagtc cgcgcgcaac acgccctgtc acgggcaggc gcagcaaggt
     9541 tttgtcccag cgcaggcgca taacaggatt tttgagtagc cgttgcccca gcaccagaag
     9601 cgccagcaat cccgccagcc agtaaacgcc gctggcctgt aacgcgtcac tcatggcgat
     9661 aagcgcgcgg gtggtggcgg gcagcgcgtg tccgaggtga tcgaactgtt cgatgatttt
     9721 cggcaccact gccgtcagca aaatagtgac cacgcccgtt gccaccacta gcagaaccag
     9781 cggatagagc atggcctgca atagacgtga tttcaggcgc tgtcgctgtt cggtgtaatc
     9841 cgccaggcga ttaagcacca cgtcgagatg cccggatttt tctccggcag ccaccatcga
     9901 acaaaacagg gaatcaaaga cgcggggatg ttcgcgcagg ctgtccgaca gggtgtaacc
     9961 ttcctgaatc cggctgcgca gcgccattcc gaggcttttt acatgcagtt tttcactttg
    10021 ctcactgacc gcctgtaagc aggtttccag cggcattgct gcctgcacca gcgttgccag
    10081 ttggcgcgtg aacagcgcca gatctgccga cgccacgcga cgatgtgcgt gtcgccgacg
    10141 ctgcaacatt ccccctgccg aagcattcat ccgggcttca atgtgcatgg gaatgagatc
    10201 tttaccgcgc aacaactggc gggcatgacg cgcggaatcc gcctcaatca tgcctttggt
    10261 tttgcgacca ttacgctcca gcgcctgata gtaaaacagt gccattacgc ctccatggtt
    10321 acccgcagaa cttcatcgag agaggtttct ccggcgagca ctttctcaat gccgttgctg
    10381 cggatgcccg cagagtgttg ccggacataa cgttccagct ccagttcccc ggcctgacgg
    10441 tggatcaaat cacgcaatgt ggcatccacc acgatcagct catggatggc agtacgtccg
    10501 cggaaacctt tatgattaca ggcggggcag ccctgcggat ggtacagagt gacggtatgc
    10561 gcgtcggtaa ttcccagcag gcgtttttct tcgtcagtgg caggcgcggg ctggcggcag
    10621 tcggggcaca gcgtgcgaac cagtcgctgc gccatcacgc ccgtcagact ggaagagagc
    10681 aggaaaggct ccacgcccat atcctgcaaa cgcgtgatcg cccccaccgc cgtgttggta
    10741 tgcagcgtgg aaaggaccag gtgtccggta agtgaagcct ggacggcgat ttctgcggtt
    10801 tcggtatcgc ggatttcacc gaccatcacc acatccgggt cctgacgcaa aatcgcgcgc
    10861 aggccacggg caaaggtcat gccgacgcgg gtgttaacct gtgtctgacc aattccttca
    10921 atcatgtatt cgatggggtc ttcaaccgtg agaatattgc gcgaatggtt gttcagctcc
    10981 tgcaatccgg cgtacagcgt ggtgcttttt ccggaacccg tcggccccgt taccagaaag
    11041 atgccgtgcg gtttgtgtaa cagctggcgc aactgcgcgg tcagttgctg gctaagcccc
    11101 agacgctcca gcgtcaggcg ggcctggttt ttgtccagca gtcgcagcac cacgcgctcg
    11161 ccccaggcgg agggcatggt ggagacacgc acgtcaatcg cccgaccgcc cagcagcagc
    11221 gcaatacgtc catcctgcgg tacgcgcttt tcggcgatat ccagccgtgc catcaccttg
    11281 atacgcgata ccagcagcga ggccagtttg cgccccgggc gcagcatttc atgtaatgtg
    11341 ccgtcaacgc gaaaacggat caccagactc ttttcaaacg tctcgatgtg aatatccgaa
    11401 gcgccttctt taatcgcctc tgccagcatg gcgttgatca gtttgatgat cggcgcatcg
    11461 tcgtcacttt ccagcagatc ttccgtttcc ggcagttctt cagcgagagt aaaaaagtcc
    11521 tcagcagaac cgagatcttc cattagctgg cgggcctcgg aagagtcccg ctgccagacc
    11581 gcattcagtc gctgttcaaa ttcggcctcg tcgatttgcc gcagcgtaaa gggcgcgttc
    11641 agcccccgct gcagctcctg caaaacagag agcgacaacg gatgcacatg gaggatctcc
    11701 agcgacgctt cgcaccatgc caccaggcta aaccgacggc tgaaactgta gggcagacgc
    11761 acggtgttag cggtggtttc ctgtgctaca ggcaccatta acgcgttctc ccggcattaa
    11821 ggaacgcacg tacttccggc ggtaaggcct ggttttgcgc tggcagtacc ggctgcgcgg
    11881 tgtgcggcat caggcttaag ccttgctcat cgcgatagat ctgttcggcg cgcatatagt
    11941 tatatttgcg ctgcgacacg ccgtctgccg ccataccgtc acgcagaatg gtcggacgga
    12001 taaacaccat caggttacgt ttttcttttt tatccgccgt cgatttaaac aggttaccaa
    12061 tcaacgggat atcgcccagc agcggcactt tcgccacact ttctcccgcc tggtcgtcca
    12121 tcagaccgcc aagcacgatc agttcaccat cgttagccag cacggtggtc ttcagtttgc
    12181 gctcaccaaa caccacgtcg aggctggtct gtccttccac cttcgagact tcctgctcaa
    12241 tcaccatctg taccgcgtta ccttcgttaa tctgcggcgt gactttcagc atgatgccga
    12301 cttttttcct ctctactgta ttgaaaggat tgctgttatt ggagccaacg gtagagccgg
    12361 ttaataccgg aacgtcctga cccaccatga agaaggcttc ctggttgtcc agcgtggtga
    12421 tgctcggcgt ggagagaacg ttcgagctgg agtcgttttt aaccgcctgt accagcgcca
    12481 tccagtcgcc tttcaccacg ccaaccgccg taccgctaaa gccggaaaga agctgggcga
    12541 gcgtggagag atcgccgttg gtatccggat taatggtggt tgcgccgttt tcactgatca
    12601 ccgtcgagcc tttctgcggt ttcgcctgag aaatggctgc gcccagcgta ccgataggga
    12661 tctgcgtacc attagcaaac tgcattaatc cggcatcttt cgacgcccac tgcacgccga
    12721 agttgatatt actgccttcg gccacttcca cgatcaacgc ctcgacgtgc acctgagcac
    12781 ggcgaatatc cagttgttca atcacgcttt gcagcgactg catgatgtcc tgcggcgcgg
    12841 ttacaatcag ggcattactg tgtttgctgg cggcgatgga gacaacctca cgcccgctac
    12901 cgaccgtgcc ttccgcctct tctttagccg ccgtgagcgt gccgctgacc tgcttcagta
    12961 catcgaccag atcttcggct ttgctgtatt tgagatagaa aacctggctg ttgccgctgc
    13021 gctccatttc tgagtccagc cgacggatca ggcggcgcat tttgtcccgc gtggccgggt
    13081 caccactgac aatcacactg ttggtgcgtt cgtcggcgac aatttgagat ttcagcgtcg
    13141 caggctggtt ctcgccgctg tttttcgtca ggctttccag cacgcgggcg atttccgaag
    13201 ccgaggcgtt atccagcggg atcacctctt cagtgcgatt acccgcgtga tccacacgct
    13261 ggatcacttc cgtcagccgc tccacgacgg aggcgcgtcc ggtgagcata atcacgttgg
    13321 agggatcgta attaacaacg ttgcctgagc ctgcgctgtc gatcatctgg cgcagaatcg
    13381 gtgccagctc gcgtaccgac acattacgta ccggcactac tttggtgacc atttcatcgc
    13441 ccgcgtagtt gtcgctgcct tcaccaacca gcggcagcgg ctcgactttc gcggcactgg
    13501 atttcaccac cttcagcacg tcgttttcca tcggtacgac cgcatacccc tgtgcttcca
    13561 gcaggttaag gaataactgg taatactggc gttcattgag cggagtcata gtgcgaatac
    13621 tcactttccc ctgaacgccc ggccccataa tgatggtttt attaaggtta gcgccgacgg
    13681 tttcgatgaa cgatttcagg tcggtatctt taaaattagc ggtgaaagtg gcttcttccg
    13741 cccagaccgg tgaactgcat aatgccgctg ccaacaccag cggcagtaaa cgcttttttg
    13801 ttttgaggcc agttgtcttc ttacgccaga ccgacaacgt aatatcacgc caaaacacga
    13861 tgaatgattc tcctggatta aatgcggtta gcgcagcgcg atggaaatgt cgtggcgcgc
    13921 acccttgcgt aaaaccgtaa gttgaatgga atccattgaa ggtaactgcc gcatcagagc
    13981 aatcattgct cgtggatcag tgaaatcctg ctgattgagc gcaatggcga tatcgccttc
    14041 cttgaaaccg ctggcatcga acagagaacg atctgccccc ggtttcactg cataaccgac
    14101 aattccctcc ttacgcacag gcgtaagctg gatatagtta aaaattttct gcggatcttt
    14161 cgccagtgcc tgacgcacgg cagtcgggat ctcaactggc gcactgacag caggttcagc
    14221 aacagcttgc tttgcttcgt cactgatggc ttttttgttg gtcacggcta cggtggaacg
    14281 ctcctcttca accaggctca ggcgctctat ttttccctga tagcgcagca tcacatggtc
    14341 gcggttgatt tcctcaatca ccgcgttgtg agaaccaagc cgttccccct gcaaatagac
    14401 ctgctgtttg ccaccttctt caataaccgc gccgggtctg gcaccaaagg cgatcccgcg
    14461 cagcaccaca ttaagacgcg tttcggccac aggtgcaggt tcgggttgtt ttaccggtgc
    14521 ggcgacgggc tgatatttgc caaaccagtt ttgctggctg attaattgca cgtcattttt
    14581 atcgaacgtt tttgcatccg tacggggcgg tttattcacc gatggagaaa ccaccgtata
    14641 ttccgcagaa aaggagaaat aacgccagag tgaatgcgcc acttttgcag aaataataag
    14701 cagcatcagc cagaacatcc cgcgtgcaat ttttcgcaga tgctctttgt ctgtattaag
    14761 agattgtctc tggttaagag tgtgacgaat ttttgtcagc cattgaatga gataaattcg
    14821 tgcgtcacga aaaacaaccc gcgccaattt atatctaccc gacgttatgc tttgactatt
    14881 ccacaggtgg tacgatccag tttccgctga tactggttac ctgtacgtgt atcattaatc
    14941 gttatgctgt aattaattcc ctcagtgagc attaatttca ccgttgggtc agcgcacatt
    15001 tgtcgttgat agctggttaa aaaggcgtct ggcgtctgcg tgatctgagt acccgcttcg
    15061 ctgataatgg tcatttttac cgtcgtgccg ctactttgtg ccagcactaa ggtatatccc
    15121 gcagatttaa tcggcaggtt ttggctgata ttttgcgcct gttttttcgc cagtaaactg
    15181 gcattttcat tatggccggc acagccactt aataatcctg taacgctcaa caatagcgat
    15241 attaataccc tccctggcat ttgttttatc gacatggtaa ataatctcta aggttattaa
    15301 taagagttaa aatgtcactt tgataatgac gtggttatca ttaaaacaat gcctgtagat
    15361 aaagtgttgc tataccgccc agacttaaac acggtccaaa aggcagtgtg gttgatcctc
    15421 tttttgtaat aacggcatat atcaggccgc agcatgaggc gattaaagca acattgggca
    15481 gtgataacgg tcccacccaa ctacctaaag cggcgaacaa taatacatcg cccatgccta
    15541 atgcttcttt acgcagaact attccggcta tccagcgcag agagtaaaaa gtgataaacc
    15601 ccaccaggac gccggtgact gcatcttgta gcgtgagcgg gctctgctgc gcccatgccg
    15661 caattagtcc cgtccacaat acgccctgag taaaaacatc gggcagccat tggtgatcga
    15721 ggtcgatgat gctcgcggca atcagccagg cggataatat catcaccgcc agcgcccatc
    15781 cactttccgg ccacaccagg ctcgccagca aaaaagcgag tgccgtcaat aactccacca
    15841 gcggataacg cttgctgatt ttcgcctgac aatcacggca gcgccctttg agcatcagcc
    15901 aggagagcag cggaatattg tcacgtatcc ggatggtctg ctgacaatgt gggcagtgcg
    15961 aacgcggtag cgcaaggctt atttttgact gcgcactcgg catttcacca tgaaactccg
    16021 ccatttgttg gcgcagcatg atggggtaac gccaaatcac cacattcaaa aaactgccga
    16081 tgatcaatcc tccgacggta gccagtatgg gcatcgccac ggggtattgc tgaaaaacat
    16141 caaaaagcat ggttaaaggt tgtttgttgt aacttgccgg atgcggcgta aaacgcctta
    16201 tccggcctac aggtatctgc caacgcaagt aatcgcggct ttccctgaag acccgataag
    16261 atggcgcatc gggcatatgt gttaacccga tgcgccttat ttcatgccgg atgcggcgcg
    16321 agcgccttat ccggcctaca ggtgtctgcc aacgcaaata aacgcagctt tccctctctg
    16381 taggcccgat aagcttgcgc atcgggcata tgtgttgacc cggtgcgcct tatttcatgc
    16441 cggatgcggc gcgagcgcct tatccggcat acaggcttac tcggctgaca tcttatgctc
    16501 ggtaacctga ttaatggttt ccggcccttg ctgcggtttc ggcagaccaa gcgccgccag
    16561 cgtctcgtaa gccgactggc tcacaccgcc ctcgaagttc atctcgctca cccccggcag
    16621 ctggtaagca ttcgcgcccg gattccattt tttaaagaac tccgaaagat ccgtctgggc
    16681 gacccaggag gcacacagca tcagcttgtc ggcagcgtta ccgttggatt cggcacagta
    16741 gtttctgttg ccaaatttgt cattgccaac atcatcgccg cgtgctttac gatgcatcaa
    16801 ctggaacagg ttccagcctt tcatcccttc acgctcgctg taaaactctg gcagaggagt
    16861 gccatctgga taccatttct tgatatcaaa gtttttctct gcccattcct tcagctgcgc
    16921 gtacatcagc aaacggtcac ccgcaccgcc gcgcgcccaa gcctggttgt tgctctcctc
    16981 cagatattcc ggcgcgacgg taatatcgtc agcgacacgg ttcatcttgc cgagatagcg
    17041 atcctgcatg tacagcgcca gcacgttgtt cgccacttcg gtcgcgcctg gaacagtcag
    17101 cggcgtttct gcggcgttgt gccctacttc gtgccagatc aaccagtcgt tcagcggcgt
    17161 cgtcggcagc gtggtgctgt tcgtcgagaa gctgctgttc attaccggat aacccgagtg
    17221 cgcatcaccg atggagatct gcacatcgtt tgtgaaacga tgtttatgtc ccgtcaatgc
    17281 tttataggta aacatccggt gcttaccgct cgtttcatca cgaccgtaga agtcattcat
    17341 cgagctggca aaggtgtcca gatcgttagc gaattgctcc agtccgccag tgtaattgct
    17401 ggcattcagg ttcttcttcg gtgtggtata gacgaacgag gcagactcca gttcgcccag
    17461 cggagccggt gagttcagat cgtttttcca tgcgccgtct ttatagaacg gtgcttttac
    17521 cacgccagta aaggtgaagc tggcagattc attggtagag ctattgccct tgatataaat
    17581 caggccaccg taaggcacct tgaacttcac cgtaccgcta gcgtccagag agtacgtttt
    17641 agtcactctt ggcggacggt tcagcgcaac ttcatgcttc tcacgtccgg tcaggtcgtc
    17701 agccagcgcc acggtgacgg tcacaggaac gttcgcattg gacttaatgg tgacctcttt
    17761 ctgagccggt gcccacaggc cggttgattg catgttaccc gcaaaccatt tggtcggatt
    17821 cgagtacagg ctgatggttt cagtaacgtt ctgtccctct tccgatactg ctcccggata
    17881 cttctccaca tcaaccttaa tgttcagatc ccaccaggaa cggcccagca tcaggcgcgt
    17941 cagcggtttt tccatatagt tgagtggata gcttgggttc atcatgcccg ctttaccgct
    18001 actttcaccg tagatcatgt tgttatcgac cagcgatttt ttcagatctg cggagcactg
    18061 tgtgccatcg gtataggcat cattggtata gcagttcagg aactcggtga acgttttaaa
    18121 gcccagctcg tcatttttgc cttcttcata acgatagctc gtatcgttcc acagccagac
    18181 cgacatgttt tggtacagac gttccagatc gacgctgctc agacgctcac ctttgcgacc
    18241 attggtccgg aagtagagct catgctgata cagacgctga atgttggtgc ctaaatctgc
    18301 agcctgcacc atcgcttttg cagtgtcggc gttaaggctt agttgcgtat actgtggaac
    18361 atacatgcca ccagtaaccg gaactcccgt gccaggacga tattccaggc agttgacctc
    18421 gtagtggtag tcagaatcct tacactcttc cagccctggg aacgcgtcca gaattctctt
    18481 cttcgccgca gccagtgact caggcgtttt gtggtcggct tcatcgataa aggcgaattg
    18541 agttacctgt ttaccctcaa cttcttcctg ccagctggca acttccagct tcggtttgtc
    18601 atcaggtttg ttttcttgct gatatttcca gataacttct ttcgtggtgt catcaatggt
    18661 atacggtggt ttaccatcga cagccggata acgctcatag acccaaattg gcgttgaacg
    18721 tcgttgacga acgcggtccg ggtagccttg cggatcgtta tttactaccg acttgttaag
    18781 cgccatcgac aaacctgcgg catcaagcag acgtacaaag ccagatgcgc tctcttcctt
    18841 aagattgctc atcacgtttt ccatgatcag caccgatcca cctttgttca tataggcgat
    18901 caaatcggtc acatcctgct gagtcagctt cggtttgctg gtatctgcac gcagcgggat
    18961 tgcataagga tcattaccaa cctgagtcac atactcaaag ccgttgagga tcagcagcgg
    19021 catttcctgc gggtcgagat cgccatagct acttaaatgc tcaacagaga tacccgcaaa
    19081 atccggatga aagccgaact cagcgctgtt tcctgtaacc tgaccatgac gtttgaaata
    19141 gacagtatcc aggttggtgc ctacggtcat gctggctttc gcgtccggcg tccatttatc
    19201 gtcggacaga tagcgcagta cgttctgcat gaagtgcttc atgtcatctg aatcaccgct
    19261 gagcgtacac tgcccgtctt tattaacgcc cccgttccag ctgtaaccgt tcgggcaacg
    19321 caaaatgctg ttgtagtgcg ggttaccgat aaccatcagt ttgccctcac cgacttgccc
    19381 cagcgaaata aacggcaggt taaaggtggc ggtatcgcgc gtaacgtttt ccggctcaac
    19441 aagagaaggc gcttccgtaa tgtacgccag ctcgttttta tcccaggcgc gtttttcgcc
    19501 aaacgccagc cagtagtttt tatcattacg cgccatcaga attgggaagg ccgcgttgga
    19561 gatattcacc accgcctgac cgcgcgcatt accagtgctg ccatagaagt tggtagagtc
    19621 atgaaagacg tggaatttcg tcaccgattt gtaatcttca tccacacccc acagcttgtt
    19681 aataacgccc tgaatctggc cgtcattaac attgcgcgtc gtcagcgaga accagcgagc
    19741 ctcgttacaa ccatcggttt tcgcacaaat cgcggtatcg atctctttgg cctgacccgt
    19801 attaaactgc tcaataaatt cgtttggcag attaacaact tgctcacctt cccccagcgt
    19861 cgcaccgttg gataacgaga gattgataat ctcgttgatc acgttgggat attcggcaaa
    19921 gaccttgcgt acatcgtccg gaacaacacg ggtattattt tgcccggtcg tcgaatagcg
    19981 atgaataagc tgatcaatat tcgccccgcg aacttcatca cccagttcag tcagcgcaat
    20041 ggtcgactta ttgccgcgca ctgaacccag ttcaaaggta tcgataccaa aggagatggt
    20101 ttcgccccag ctaaaggaaa attcaccatt ttcccctgtc acgccacggc ctgaattggt
    20161 gtagtagttg acgccagcaa caccatatcc ctggctatcg accaggcgac cttctgagag
    20221 aatgatttcc gagggttgat actgataaaa ctgttccgcg ttagccgaca cgaaggaagc
    20281 gttcagatcc ggttttgttc ccggcgtggt gacgggcacg accggtgaag tatgagtgga
    20341 tggcgcttta tcggtcgcag cattgttttc cacctcttca ttgaccagct ttttgaactc
    20401 ttccggtgcc agatcgattt gcttatacag cgagtcgaag cgtttactct cgatcaccga
    20461 ggagaacgtc agacaaacct gttctgtatt cgccggacag ctgttactgg acgttaccag
    20521 cgaaaccgca ttgcttttct tgtcatcgga gcccgccagt tcttgcgcgt cctcaaggct
    20581 aaacgacact ttttcaaccg cacgcaagct acgcgcagct tctgactgag tgttgaaggt
    20641 ggcaattgtc gtgttacccg ccacgcaagt aacgtcctcg ccaggtttaa atgtaaagcc
    20701 atcgctggat tcaccattac aggtagcacc agttacccgc tggcttccgc ccagggtcag
    20761 ataacccgtt ttcgtaggaa caggttctgg ctccggttct ggtgttggtt caggatcagg
    20821 tatcggttct ggcgtaggct ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg
    20881 tgttggatca ggtttcactt ccggcaaaga ccctgttcca gaatctacag gcggcgtatc
    20941 ggaggaagat ccggagccac cgccatcaca accggctaac agggttgcac tcaaaatagc
    21001 cgctaaaagc gatttcttat atttaaattt cttattcatt aataacgcaa gtgacaaaac
    21061 aatgttaaaa aattcgtaac tgggagaaat agttttatgc tttatcttct ttaataattt
    21121 cctccattct
//
LOCUS       DAZGCQ010000087        20755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000087 DAZGCQ010000000
VERSION     DAZGCQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.087"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1034
                     /locus_tag="ACHK07_003590"
     CDS             3..1034
                     /locus_tag="ACHK07_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO8103874.1"
                     /translation="MDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQGKHGATLAD
                     ITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRVNMDFTQDVC
                     YQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHYYSDGAFNAA
                     LPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKENIANAGFSD
                     RIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKPGAKVCIMEL
                     FWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVAERHDNLGVG
                     HTLLICQKK"
     gene            1075..1797
                     /locus_tag="ACHK07_003591"
     CDS             1075..1797
                     /locus_tag="ACHK07_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO8103875.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            1794..2615
                     /locus_tag="ACHK07_003592"
     CDS             1794..2615
                     /locus_tag="ACHK07_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO8103876.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            2590..2847
                     /locus_tag="ACHK07_003593"
     CDS             2590..2847
                     /locus_tag="ACHK07_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO8103877.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            2859..3110
                     /locus_tag="ACHK07_003594"
     CDS             2859..3110
                     /locus_tag="ACHK07_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8103878.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPKDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            3115..3696
                     /locus_tag="ACHK07_003595"
     CDS             3115..3696
                     /locus_tag="ACHK07_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103879.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQPRRQAGPLQAVTQIVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            3693..5054
                     /locus_tag="ACHK07_003596"
     CDS             3693..5054
                     /locus_tag="ACHK07_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFO8103880.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQCIGVLLVLDDE
                     AHLQWQNGGGHSQEQAWRRLLHPMLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            5041..5394
                     /locus_tag="ACHK07_003597"
     CDS             5041..5394
                     /locus_tag="ACHK07_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO8103881.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            5385..7061
                     /locus_tag="ACHK07_003598"
     CDS             5385..7061
                     /locus_tag="ACHK07_003598"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8103882.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7065..7487
                     /locus_tag="ACHK07_003599"
     CDS             7065..7487
                     /locus_tag="ACHK07_003599"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO8103883.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7484..8089
                     /locus_tag="ACHK07_003600"
     CDS             7484..8089
                     /locus_tag="ACHK07_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO8103884.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            8058..10376
                     /locus_tag="ACHK07_003601"
     CDS             8058..10376
                     /locus_tag="ACHK07_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO8103885.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVVLALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTTSLENA
                     KKEGLISNYRTIPLNSLARQEEDLHLLTTAAPTVTKALQNAGLTTVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKNSAALQEISSYYPCGIAWVDRKNTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10373..10957
                     /locus_tag="ACHK07_003602"
     CDS             10373..10957
                     /locus_tag="ACHK07_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO8103886.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            10959..12128
                     /locus_tag="ACHK07_003603"
     CDS             10959..12128
                     /locus_tag="ACHK07_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO8103887.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQIDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSPTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQLIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12125..12589
                     /locus_tag="ACHK07_003604"
     CDS             12125..12589
                     /locus_tag="ACHK07_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO8103888.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            12589..13320
                     /locus_tag="ACHK07_003605"
     CDS             12589..13320
                     /locus_tag="ACHK07_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO8103889.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13317..14546
                     /locus_tag="ACHK07_003606"
     CDS             13317..14546
                     /locus_tag="ACHK07_003606"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO8103890.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            14548..15135
                     /gene="acpT"
                     /locus_tag="ACHK07_003607"
     CDS             14548..15135
                     /gene="acpT"
                     /locus_tag="ACHK07_003607"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO8103891.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            15246..16820
                     /gene="nikA"
                     /locus_tag="ACHK07_003608"
     CDS             15246..16820
                     /gene="nikA"
                     /locus_tag="ACHK07_003608"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8103892.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            16820..17764
                     /gene="nikB"
                     /locus_tag="ACHK07_003609"
     CDS             16820..17764
                     /gene="nikB"
                     /locus_tag="ACHK07_003609"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO8103893.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            17761..18594
                     /gene="nikC"
                     /locus_tag="ACHK07_003610"
     CDS             17761..18594
                     /gene="nikC"
                     /locus_tag="ACHK07_003610"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO8103894.1"
                     /translation="MNFFISSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            18594..19358
                     /gene="nikD"
                     /locus_tag="ACHK07_003611"
     CDS             18594..19358
                     /gene="nikD"
                     /locus_tag="ACHK07_003611"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO8103895.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            19355..20161
                     /gene="nikE"
                     /locus_tag="ACHK07_003612"
     CDS             19355..20161
                     /gene="nikE"
                     /locus_tag="ACHK07_003612"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO8103896.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLERASEMLKAVDLDDSVLNKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            20167..20568
                     /gene="nikR"
                     /locus_tag="ACHK07_003613"
     CDS             20167..20568
                     /gene="nikR"
                     /locus_tag="ACHK07_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO8103897.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT         4464 a         5843 c         5762 g         4686 t
ORIGIN      
        1 cactggatgc gattaccgaa gctcaacgca ttgcctttgc ccctatgctt tttcaaacag
       61 cactctgcct acgtaatgcg ggtgtacttt catatctgga tcggcaaggc aaacatggcg
      121 caacgctggc tgatatcacc gaacatagcc atatcaacga atatgctgcc agcgttctgt
      181 tagatatggg cttaagcggg cgaattatta cctgtaaaga gggaatttat tacctggcga
      241 aaatcggtca ttacctcctg catgacacta tgactcgcgt gaatatggat ttcacccagg
      301 atgtctgtta ccaggggctc ttcttcctcg ccgattcact caatgaagga aaaccttccg
      361 ggttaaaagt atttggcgac tggtcgacga tatatccggc actttcgcaa ttaccagacg
      421 ccgctcgtga aagctggttt gcgtttgatc attactattc agatggcgca tttaacgccg
      481 cactgcctta tgtattcgcc aacaacccca caacattgta cgatgtaggc ggcaatacgg
      541 gtaagtgggc tttgcgttgc tgcaaataca atgaaaacat cgccgtgacg ttattagatc
      601 tgccacaaca aatcgtacta gcgaaagaaa atatcgcgaa tgcaggtttt tctgatcgca
      661 tagatttcca tgcggtggat atgctaagcg atgccccatt accgggcgag gctgatatct
      721 ggtggatgag ccaattttta gactgttttt cacccgaaca aataattagc atactcagca
      781 aaattgccag cgtaatgaag cctggcgcaa aagtttgcat catggagttg ttctgggatg
      841 cgcagcggtt tgaagcagca tcatttagcc tgaacgcgtc ctcactttat tttacctgca
      901 tggcaaacgg taacagccgt ttctatagtg cagagaaatt ttacgactat ctgaataaag
      961 caggattcca ggtagcagag cgccacgata atttaggcgt tgggcataca cttttgatat
     1021 gccaaaagaa ataatcgtca cttaataatt ttggtttacc gcgggcgcgc gttgatgaaa
     1081 tttacattaa atattaccga ctggcaggca ctggcgcctg gacttagcga agcgcagcaa
     1141 tggcaagcat ggtcgcgcca gccgtgggcg attgaccctg ccgcgccgct ggcaaaatta
     1201 agtgaactgc caatgatgac cgcccgccgc ctcagttcag gcagcaaact ggctgttgaa
     1261 tgcggtctgg caatgctacg ccgccatcag ccagacgccg ttttgtatac cagtcgtcat
     1321 ggcgaactgg agcgcaatta ccgcattgtt catgcactgg caaccgaaca ggcgctttct
     1381 ccgaccgact ttgcactttc agtacacaac tcttccgtgg gtaacctgac cattgcggcc
     1441 aaacagccga tcgtttcgtc atcgctttct gctggtcgcg acactttcca gcaaggtttg
     1501 tgtgaagtgc ttagcctgtt acaagcggga taccagcgcg tgctgatggt cgattttgac
     1561 ggtttcctgc ccgagtttta tcacccacaa ctgcctgcag aaatgccgac ctggccgtac
     1621 gccgtcgcgc tggtgattga agctggcgat gactggcaat gtgaaacgca gtcagccatt
     1681 gcgggcaatg aaacaacact gccgcaaagc atgttgtttt tacagcacta tttacaaaat
     1741 gtagacacat tttcactgcc tggcgagcgc gtgcaatggc gctggagcca cagatgagta
     1801 agctgatgtc acgtctggag tggacctggc gactggtcat gaccgggtta tgttttgccc
     1861 tgtttggtct cggcggcctg ctgctttctg tcgtgtggtt taacatattg ttagttttgg
     1921 tgtgggatac ttcccgccgc cgtcgtttgg cgcgtcgcag cattgccgcc agttttcgcc
     1981 tgtttttaac tgtcgcgaaa gggcttggcg tgctggatta tcgtattgat ggcgctgaaa
     2041 ttttacgtca ggaacgcggt tgcctcgtcg tagccaatca tcccacgctg atcgattacg
     2101 tgctgctggc gtcagtcatg ccagaaactg actgcctggt gaaaagcgca ctgttaaaaa
     2161 atccttttct tggcggcgtc gtgcgggcgg cagattactt aatcaacagc gaagctgaaa
     2221 ccctgttacc ccgctgccaa cagcgactgg cacagggtga cactatcctg attttccccg
     2281 aaggcacacg aacgaagccc ggtgaaaaaa tgacgctgca gcgcggggcg gcgaatattg
     2341 ccgtgcgctg cgccagcgat ttacgcatcg tgacaatcgg ctgtagcgag catctgctgg
     2401 ataaacaaag taaatggtat gacgttccac ctgaccggcc gttttttacg gttgcagtcc
     2461 atggtcgggt tgaaatcaat aaattttacg atgcaacttc acaagaaccg gcgctggcag
     2521 caaggcagtt aaaccggcat cttttgcttc aattacaaca aggttgttta cctctctcag
     2581 gaataaataa tgcaagcgct ttatctggaa attaaaaacc tcatcatcag caccctcaat
     2641 ctggacgagt tgacgcctga tgatattgat accgacgcgc cgctgtttgg cgacggttta
     2701 gggctggact ctatcgatgc cctggaactg ggactggcag taaaaaatga gtacggcatc
     2761 gtgctctctg cggaaagcga agagatgcgt cagcactttt tctccgtcgc cactctggcc
     2821 tctttcattg ctgcgcaacg tgcctgagga aagccatcat gacagaacaa caaaccgtct
     2881 atcaggaagt ctcagccctg ctggttaagc tgtttgaaat cgatccgaag gacatcaaac
     2941 ctgaagcgcg cctgtacgaa gatctggaac tggacagcat cgacgccgtt gacatgattg
     3001 tgcacctgca aaagaaaacc ggtaagaaaa tcaagccgga agagtttaaa gcagtgcgta
     3061 cagtccagga tgtcgtcgag gctgtagaac gcctgctaca agaagcctga tctgatgtcg
     3121 ggcattcgct cgctaccgat gattaagctg ctgactggat tattgctgct cgcctggccg
     3181 tttgtgattt ggtttggctt agcgcataac ggcctgcact ggctgttacc gctaatggcg
     3241 ttgctgcttt tgttgcgtct gcgccagccc cgtcgccagg cggggccttt acaagccgtc
     3301 acacaaatcg tcgctgtcgt gggcatcgca ttatgtgtcg ccagctttat gttgaaaacg
     3361 caccagctac tgctgtttta cccggtagtg gttaacgcgg tcatgctggc ggtttttggc
     3421 ggttcgctgt ggtcggcaat gccgatagtt gagcggctgg cgcggttgca ggagccagac
     3481 ctcccggaaa agggcgtgcg ctatacccgc cacgtcacgc aaatctggtg cggctttttc
     3541 atcattaacg gtggaattgc cctctttacc gcgctttatg ccgatatgtc gttatggacc
     3601 gcctggaacg gcatgattgc ttatttgttg atggggacgt taatggctgg tgaatggctg
     3661 gtgcgccgcc aggttatgaa aagagatcgc gcatgaagca gaccttaccg cttgcacgct
     3721 ggctgactgc gccacgccct gatgacacac ccattgcgtg gctgaacgaa agtacctgga
     3781 cgctgggcga tttgcgtcac gatgtcgcgc aacttatctg ccgcttgcag caacagccgg
     3841 gtgaacgctg ggcgctgtgc tttgagaaca gctatctgtt tatcgttgcc ctgctcgcca
     3901 cgctacacgc cggaaaaaca ccggtgcttc cggggcataa ccgtgtcatc cagcttaacg
     3961 agcaacgtga actgttcgat ggtgtgctta gcgacagcga acttaactgg caaggttcat
     4021 tgctgttggt agcaagttct ccacaaatcg cgacacaatc cttcaccttt gccgctatag
     4081 cgccagaggc ttttatcgag ctgtttacct ccggttccac gggacagccg aaacgggtga
     4141 ttaaacctgt tcatttgctg gatcgtgaag cagaactgct ggctgaacga ttaggcgcac
     4201 gtctggcggg ttgtcgtgtc gtcggttccg tattgccgca gcatttgtac ggcctgactt
     4261 ttcgcgtttt cctgcccatg gcgttgggat taccgctgca cgccgccatg ctctggtatg
     4321 tcgaacagtt tgccgcgttg agtcatcagc atcgctatat cttcatcagc agcccggcat
     4381 ttttgaagcg cctggatacc cagctttctc cgccacccgt tcaggtgctt ctctctgctg
     4441 gcggtgagct gccgtggcag gatgtacaac acaccgcaag ctggctgcgc gtctggcctg
     4501 atgaaatcta cggcagcacg gaaaccggcg tcatcgcctg gcgctaccgc gaacaagagc
     4561 aacgccgctg gctacccctc ccaggtatgc agttccaggc cgaaggcgat gcctttcgcc
     4621 tcttttcacc gctgatagcg gaagataacg gcctgttact cgacgatatc ttacagttta
     4681 gcgaagatgg tcagttccat ctgatggggc gtcgtgggcg catcgttaag attgaagaaa
     4741 aacgtatttc tttgcaggaa gtggaacaac gtctgctggc actggacggc attcgcgagg
     4801 cggcggcagt tcctgttaca cgcggcgggc gtcagtgcat tggcgttttg ctggtgctgg
     4861 atgatgaagc tcatctgcaa tggcagaacg gcggcgggca tagccaggag caggcctggc
     4921 ggcgattact gcacccgatg ctggagccgg ttgctattcc gcgttactgg cgcgttattg
     4981 atgaaatgcc agtaaacagt atgaacaagc gtgtctatgc gcaattacag gagttatttc
     5041 atgaagcccc atgaaatcga gcgccatcag gcacaaacga atcaccttga aattgttctg
     5101 catctcaggg cggatctgtt ctggtttcgc ggtcattttg ccgtgcaacc attactccca
     5161 ggagttgcgc aaatcgactg ggcgatgagc tacgcgctca atctgctcgc gcctggctgg
     5221 cgttttcact ccattcagaa cataaaattc cagtctccgt tgctgccgga aaaccgcgtc
     5281 acgctcgcgc ttaactggca ggaagaacgc cagattctga gctttagcta tcagcgtcac
     5341 gacggtgacg cccgccacac cgccagtagc gggaagatcc gcctatgtcg gtaaactttt
     5401 ctccctgcgt gttgataccc tgctacaacc acggcgcgat gatgccgggc gtgctggcgc
     5461 gtcttaagcc atttaatctg ccctgtattg tagtggatga cggcagcgat gccgccacgc
     5521 aacagcaact ggacagtctg cttgccgaac agcctggcgt gaccttaatt cgcttagcag
     5581 aaaacgcagg taaaggcgcg gcagtgatgc gtggcttaca ggcagcggca gacgcagggt
     5641 tcagccatgc ggtgcaggtg gatgctgacg gtcaacatgc gattgaagat atccccaaac
     5701 tgctggcact cgctgaacaa caccctgcgg cgctgatctc cggccagcca atttacgatg
     5761 actccatccc ccgctcacgg ctttacgggc gctgggtcac ccacgtctgg gtatggatcg
     5821 aaacgctctc cctgcaactg aaagacagca tgtgcggctt tcgcgtttat ccggttgcgc
     5881 caacgctgca actggcaaaa cacgccacca tcggcaagcg gatggatttc gacactgaag
     5941 tgatggtgcg cctctactgg cagggaaaca ccagctattt cgtgccgacc cgcgtcacct
     6001 atccactgga cgggctttcg cattttgatg ccctgaaaga taacgtccgc atctcgctca
     6061 tgcacacgcg tctgtttttc ggcatgttgc cgcgtattcc ttcactgctg atgcgccgct
     6121 cttccagcca ctgggcgcgg cagagtgaag tgaaaggatt atggggaatg cgcctgatgc
     6181 tgctggtctg gcgtctgctg ggaagaacgg cgtttagcgc gctgctttac ccggtggtgg
     6241 gcgtctactg gctcactgct tctcgtgcgc gcaaagcgtc gcaagactgg ctcgcccgtg
     6301 tacgacagca tcaaccacag gcggcaaaac tcaacagcta tcagcacttt ctacgtttcg
     6361 gtaatgccat gctcgacaaa atcgccagct ggcgcggcga gctacaactg gggcgtgatg
     6421 tgctgtttgc gccaggcgca gaagcggcgc ttgacgtccg cgatccgcgc ggcaaattgc
     6481 tgctggcctc gcatcttggc gatgtggaag tgtgccgggc gctggcaaaa attcagggct
     6541 acaaaaccat taacgcgctg gtgtttagcg aaaacgccca acgctttaaa cagataatgc
     6601 aggagatggc tcctcaggca ggcattaacc tgatgccggt aacagatatc ggcccggaaa
     6661 ccgccatcct gctgaaagag aagctggata acggcgaatg ggtggcgatt gtcggtgacc
     6721 gcatcgccgt caccccgcaa cgcggcggcg actggcgcgt ctgctggagt ccgtttatgg
     6781 gccagcctgc gcccttccca caggggccgt ttattctcgc ctctattttg cgctgcccgg
     6841 tgaatctgat tttcgcccta cgccagcatg gcaagctgca tattcactgc gaaacctttg
     6901 ccgacccgct gctgttgccg cgtggcgaac gccaacaggc gctgcaaaac gctatcgacc
     6961 actacgccgc gcgtctggaa cattacgcgc tccagtcgcc cctcgactgg tttaattttt
     7021 tcgatttctg gcaactgccg gaaattcagg acaaggagta aagggtgctt aacgatcccc
     7081 gatttaccgc tgaagtcgag ctgaccattc cctttcacga cgtcgatatg atgggcgtgg
     7141 cctggcacgg taactatttt cgctactttg aagtggcccg cgaggcgctg ctcaaccagt
     7201 tcaattacgg ctatcggcag atgaaagagt ccggttattt gtggccagtg gtcgatgcgc
     7261 gggtgaaata tcgccacgcc ctcacctttg agcaacgcat ccgcgtgcgt gcgcatatcg
     7321 aagagttcga aaaccgtctg cggattggct atcagatttt cgatgccgaa acgggcaaac
     7381 gcgccaccac cggatacacc attcaggtcg cggtcgacga gcaaagtcgc gaactgtgct
     7441 ttgtcagccc cgatattctg tttgaacgca tgggagtcaa accatgaaat ttttaccgct
     7501 gctggcgctg ctgattagcc cgtttgtgag cgccctgacc ctggacgatc ttcagcaacg
     7561 ctttaccgaa caaccggtga tccgcgccca ttttgatcaa acccggacga ttaaagatct
     7621 gccgcagccg ctgcgatctc agggtcagat gttgatcgcc cgcgaccagg ggttattgtg
     7681 ggatcaaacc tcaccgttcc ccatgcagct attgctggat gataaacgca tggtgcaggt
     7741 gatcaacggt cagccgccgc aaatcatcac ggcagaaaac aacccgcaga tgttccagtt
     7801 taaccacctg ctgcgcgcgc tgttccaggc cgatcgcaaa gtgctggaac aaaacttccg
     7861 cgtcgaattt gctgacaaag gcgaaggccg ctggacgctg cgcctgacgc cgaccaccac
     7921 gccgctggat aaaattttca acaccatcga tctcgccggg aaaacctatc tggagagcat
     7981 tcaacttaat gataaacagg gcgatcgcac cgatattgct cttacccaac atcaactgac
     8041 gccagcgcaa ctgaccgatg acgaacgcca acgttttgcc gcccagtaaa cgccccgcgc
     8101 tgttatgggg gctagtctgc ctggtcatgg cggcggcgtt gctgatcctg ctgccgcaat
     8161 cacggctgaa cagtagcgtg ctggctatgt tacccaaaca ggctatgggc gatattcccc
     8221 cagcgctgaa tgacggcttt atgcagcgtc tggaccgcca actggtgtgg ctggtcagcc
     8281 ccggtaaaga ggctaatccc agggtcgctc aggagtggct gacgctgctg caaaaatccg
     8341 ctgcgctcgg cgacgttaaa gggccaatgg atgccgccag ccagcaagcg tggggagcgt
     8401 ttttctggca gcaccgcaac ggcctgattg accccgacac ccgcgcgcgc ctgcaaaacg
     8461 gcggcgaagc gcaggcgcag tggatcctct cccagcttta ttctgcgttc tccggggtaa
     8521 gcggcaagga gctgcaaaac gatccgctga tgttaatgcg cggctcgcag ctggcgatgg
     8581 cgaaaaacgg ccagcgtttg cggctgatgg acggctggct ggtgacgcag gatccccagg
     8641 gcaactactg gtatctgctg cacggtgaac tggcgggatc gtcgtttgat atgcagcaaa
     8701 cccaccagct catcacgacc ctgaatacgc tggaaaagga tctgaaaacg cgttacccgc
     8761 aggcgcagct gctttcgcgc ggcacggtgt tttacagcga ttacgccagc caacaggcga
     8821 agcaggatat ctcaaccctg ggcgtggcaa cgctgctggg ggtgatattg ctgattgtgg
     8881 cggtgttccg ctctttgcgc ccgttgctgc tttgcgtgat ttccatcggc atcggcgcgc
     8941 tggcgggaac ggtcgccact ttattgattt tcggtgaatt acacctgatg acgctggtga
     9001 tgagcatgag cgttatcggc atttccgctg actacacgct ctattatctc accgagcgga
     9061 tggtgcacgg caacgacgtt tcaccgtggc aaagcctggc gaaagtgcgc aatgccttgc
     9121 tgctggcgct gctcaccacc gtggcggcgt atctgattat gatgctcgcc cccttccccg
     9181 gcattcgcca gatggcgatt tttgccgccg tcgggttgag cgcctcctgt ctgaccgtcc
     9241 tgttctggca tccgtggctg tgccgtggcc tgccggtgcg tccggttccg gcgatggcgc
     9301 tgatgctacg ctggctggca gcgtggcggc gcaataaaaa actgtcgcgg ggtctgcccg
     9361 tcgtgctggc gctgttttcg ctggcgggga tgtcaatgct gcgcgtcgat gacgatatct
     9421 cgcagttaca ggcgctaccg cagcatattc tggcccagga gaaagccatc accgctctga
     9481 ccgggcagag cgtcgatcaa aaatggttcg tggtttacgg cgactcccca cagcaaacat
     9541 tgcggcgact ggagaaattt accacctcgc tggagaatgc gaaaaaagag gggcttatca
     9601 gcaactaccg caccattccg ctgaactccc tggcgcggca ggaggaagat ttacatctgc
     9661 tgacaacagc ggccccgaca gtaacaaaag cactgcaaaa tgccgggctg acaacagtga
     9721 acccggatct caacgccatg ccggtgaagg ttgatgaatg gctggcaagc cccgccagtg
     9781 aaggctggcg tctgctgtgg ctgacgctgg aaaatggcga aagcggcgtg ctggtgccgg
     9841 ttgaaggggt taaaaatagc gcggcgctac aggaaatctc cagctattac ccttgtggca
     9901 ttgcctgggt tgatcgcaaa aacacctttg atgaattgtt cgcactttac cgttatgtgt
     9961 taaccggctt gttgctggtg gcgctggcgg tgattgcctg cggcgcagtg gcccgtctcg
    10021 gctggcgcaa agggcttatc agcctggtgc cttcggtgct ttcgctgggc tgcggtctgg
    10081 cggtgctggc gatgagcggg caggcggtga atctcttttc gctgctggcg ctggtgctgg
    10141 tgcttggcat cggcatcaat tacacgctgt ttttcagtaa tccgcgcggc acaccattaa
    10201 cttcgctact ggcgatcaca ctggcaatgc tcaccacctt gctgacgctg ggaatgttgg
    10261 tattcagcgc cacccaggcc atcagcagtt ttggcattgt gctggtgagc ggtattttca
    10321 ccgccttcct gctttcgccg ctggctatgc ccgataaaaa gagaacaaaa aaatgatcaa
    10381 atccacattc tggcgagcgt tcgccctgac cgctacgctt atcctcaccg gctgtagcca
    10441 ctcgcaacca gaacaggaag gccgcccgca ggcgtggctg caacctggca cgcgcatcac
    10501 gctgccggca ccagggattt cacccgcagt caattcgcag caactgttga ccggcagctt
    10561 caacggcaaa acccagtcgc tgttagtgat gcttaacgcc gacgatcaga aaatcaccct
    10621 tgccgggctg tcgtcggtcg gcattcgcct gtttctggtg acctacgatg cgcaagggct
    10681 acgcgccgag caatccatcg tcgtcccaca gttaccgccc gcaagtcagg tactggctga
    10741 cgtgatgctc agccactggc cgattagcgc ctggcaaccg caacttcccg caggatggac
    10801 acttcgcgac aacggtgaca aacgcgagct gcgtaacgcc agcggcaaac tggtcacgga
    10861 aatcacctat ctgaatcgcc agggaaaacg cgtgccaatc agcattgagc agcatgtctt
    10921 taaataccac atcaccattc aatacttagg tgactgatat gatttatatt tccgccgtcg
    10981 gcatgatcaa cgccctggga aataaccttg atgaaatcgc aaccaacctg gctcgcggtg
    11041 tcgcccccgg aatgcgccca cgcgcaggct ggttgcaggg gcatccgcag gcggtcctgg
    11101 ctggcgtgga tggcgagctt ccggttatcc cggaaaaatt cgctgcgcac cgatcgcgca
    11161 acaatcagat cctgctggcg gcgctggcgc agatccagcc gcagattgat gacgccattg
    11221 cgaaatatgg ccgcgagcgc attgcgattg ttcttggtac cagcacctcc ggcctgcatg
    11281 aaggcgacac gcacgttaac ctgcgcaccc acgggcagcc gagccccaca tggcactacg
    11341 cgcaacagga actcggcgat ccgtcgcgtt ttctcagcca ctggctggcg ctcgacggcc
    11401 cggcctatac cctttcaacc gcctgctctt ccagcgccag agcgataatc agcgggcgca
    11461 ggcttatcga ggcggggctg gtcgatgccg ccattgtggg tggcgcagac accctgagcc
    11521 ggatgccgat taacggtttt cacagcctcg aatcgctgtc gccaacctta tgtcagccgt
    11581 ttggtcgtga ccgcgcaggt atcaccattg gtgaaggggc ggggctaatg ctgctgaccc
    11641 gggaaccgca gctcatcgca ttgctgggcg ttggtgaatc gagtgacgct taccatattt
    11701 cggccccgca tccgcagggc gaaggggcga tccgcgccat taatcaggca ctgaccgatg
    11761 cgcagcttac gccagatgac gtcggctata tcaacctgca cggcaccgcc acgcaactca
    11821 acgaccagat tgaatcgatg gtggttaacg ccctgtttgg cgaacgcgta ccgtgcagct
    11881 ccaccaaaca tttgaccggc cacacgctgg gcgcggcggg cattaccgaa gcagcaatca
    11941 gtatgttgat cttacagcgt gatttaccgc tgcccgccca agatttcagc ctgtcgccac
    12001 gcgatcccac gctgccaccc tgcggcatta tcgaaaaacc gcagccgctg gcacgtccgg
    12061 tgatcctgtc caattccttc gcctttggcg gcaataacgc cagcattctg ctcgggaggg
    12121 tttcgtgagc cactatttat cccccggcgc ttatctgccc cacgacgcgc ccatgttact
    12181 gctggaagac gtggtgagcg tcagcgatga cagcgcggtg tgccgcgtga cggtttcgcc
    12241 cagtggcgta ctggcaccgt ttctcgaccc ggacggcaat ctccccggct ggtttgccct
    12301 tgaactgatg gcgcaaaccg tcggcgtctg gtctggctgg catcgccacc agcagggcaa
    12361 aaacagtatt gagttaggga tgatactggg cgcgcgtgaa ttgctttgcg ccgctggcat
    12421 tctgcccgca ggccagacgc tgaccattac cgtcaaactg ctgatgcagg acgatcgctt
    12481 cggcagtttt gagtgcagca tcaacgacgg cgaggcgacg ggccgcatta acaccttcca
    12541 gccgaccgcg gaagaactta ccaccctgtt tcaacaagga gcgtcctgat gagtcgttca
    12601 gttctggtta ctggtgccag caaaggcatt ggtcgcgcca tcgcctgcca actggcggca
    12661 gacggcttta acattggcgt tcactatcac cgtgacgcca caggcgctca ggaaacgctg
    12721 aatgccattg tcgccaacgg gggtaacggg cgtttgctca gtttcgacgt cgcaaaccgc
    12781 gaacagtgtc gggaagtgct ggagcatgag atcgcgcaac acggtgcctg gtacggcgtg
    12841 gtcagtaacg ccgggatcgc ccgcgatgcc gcttttccgg ctttaagcga tgacgactgg
    12901 gatgcggtga tccacaccaa cctcgacagt ttctacaacg tcattcagcc gtgcattatg
    12961 ccgatgatcg gcgcacgcca gggtgggcgc atcatcacgc tatcctcggt ttccggcgta
    13021 atgggcaatc gcggccaggt gaattacagc gctgccaaag ccggaattat cggcgcaaca
    13081 aaagcgctgg ctatcgaact cgcgaagcgc aaaatcaccg tcaactgcat cgcgccgggg
    13141 cttattgata ccgggatgat cgaaatggaa gagagcgctc tgaaagaggc aatgtcgatg
    13201 atccccatga aacgtatggg ccaggcggaa gaagttgccg ggcttgccag ttatttaatg
    13261 tctgatattg cgggttacgt cacccgccag gttatttcca tcaacggagg gatgttatga
    13321 cacgtcgcgt agtgattaca ggaatgggcg gcgtgactgc ctttggtgaa aactggcagg
    13381 acgtttctgc acgcttactg gcgtatgaaa atgcggtgcg caaaatgccg gagtggcagg
    13441 tctacgatgg cctgcacacc ctgcttggcg cgcctgttga tgatttcacg ctaccggagc
    13501 actacacccg caagcgtatc cgtgcgatgg gccgcgtgtc gcaaatgtcg acccgtgcca
    13561 gcgagctggc gttagagcag gcagggttga ttggcgatcc gatattaacc agcggcgaaa
    13621 cgggcattgc gtacggttca tcgaccggca gtaccggccc ggtaagcgag ttcgccacca
    13681 tgctgacgga aaagcacacc aataacatca ctggcaccac ctatgtgcag atgatgccgc
    13741 acaccaccgc cgttaatact ggcctctttt tcggcctgcg cgggcgggtg atcccaacct
    13801 ccagcgcctg tacttccggc agccaggcaa tcggttatgc gtgggaagcc attcgccacg
    13861 gttatcaaac ggtgatggtt gctggtggtg ccgaagagtt atgtccttca gaagcagcgg
    13921 tatttgatac gcttttcgcc accagccagc acaatgatgc gccgaaaacc acaccttcac
    13981 cgttcgatga aaaccgcgac gggctggtga ttggtgaagg cgcaggtacg ctcattctcg
    14041 aagaactgga gcacgccaaa gcgcgcggcg cgaccattta cggtgaaatc gtcggctttg
    14101 cgactaactg cgacgcggca catattaccc agccgcagcg cgaaaccatg cagtattgta
    14161 tggagcaatc gctgaaaatc gccgggttaa gcgcacaaga tatcggctat atttcagcgc
    14221 atggtacggc gaccgatcgc ggtgatatgg cagaaagtct ggcgacagca accatttacg
    14281 gtgataatgt gccgctctcc tcgctgaaaa gttattttgg tcatacgctt ggtgcctgtg
    14341 gcgcgctcga agcctggatg agtctgcaaa tgatgcgcga aggttggttt gcgcctacgc
    14401 taaacctgaa taaaccagac ccgaattgtg gcgcgctgga ttacatcatg catgaagccc
    14461 gcaaggttga ttgtgagttt ttgcagagta acaactttgc ctttggcggc attaatacat
    14521 ccatcatcat taaacgttgg ccctgatatg tatcggatag ttctggggaa agtttcgacc
    14581 ttaagcgcag ctccactgcc accgggttta cgcgagcaag caccgcaagg tccacgacgc
    14641 gaacgctggc tggcggggcg tgcattgctt tcgcacacgc tttccccgct accggagatc
    14701 atctatggcg aacaaggcaa acctgcattc gcgccggaaa cgccgctatg gttcaactta
    14761 agccatagcg gtgacgatat cgccctgctg ttgagtgatg aaggcgaagt cggctgcgat
    14821 atcgaagtga ttcgcccgcg cgccaactgg cgctggctgg cgaacgccgt gttcagcctc
    14881 ggggaacacg ctgaaatgga cgcagtgcat cctgatcagc aactggaaat gttctggcgc
    14941 atctggacgc gcaaagaagc catcgttaaa cagcgtggcg gcagcgcctg gcaaatcgtc
    15001 agcgtagata gcacctatca ctcctcgctg tcagtcagcc attgtcagct agaaaattta
    15061 agccttgcga tctgcacccc cacccccttt acgctcaccg ccgacagtgt gcaatggatc
    15121 gattcagtta actgatccgc ccacccgact gcccatctat tgatccagaa caggtaatca
    15181 gtatgacgaa tacttaaaat cgtcatactt atttccgcca tctattttaa tccattgggg
    15241 ttaccatgct ctccacactc cgccgcactc tatttgcgct gctggcttgt gcgtctttta
    15301 tcgtccatgc cgctgcacca gatgaaatca ccaccgcctg gccggtgaat gtcgggccac
    15361 taaacccgca cctttacacg cctaaccaga tgttcgccca gagcatggtt tatgaaccgc
    15421 tggtgaaata tcaggctgat ggttcggtga tcccgtggct ggcaaaaagc tggactcatt
    15481 cagaagatgg taaaacctgg accttcaccc tgcgtgatga cgtgaaattc tctaacggcg
    15541 aaccgtttga tgcagaggcg gcggcagaaa acttccgcgc agtgctcgat aaccgtcaac
    15601 gtcacgcctg gctggagctg gcaaaccaga ttgttgatgt caaagcactc agtaaaacag
    15661 agctgcaaat caccctgaaa agcgcctact atcctttcct gcaagaactg gccctgccgc
    15721 gccctttccg ctttatcgcc ccctcgcagt ttaaaaacca tgaaaccatg aacgggatta
    15781 aagcgccgat tggcaccggg ccgtgggttt tgcaggaatc gaaactgaat cagtacgatg
    15841 tcttcgtccg taacgaaaac tactggggcg aaaagccggc aattaagaag atcactttta
    15901 acgtcatccc ggacccgact acccgcgcgg tggcgtttga aactggcgat atcgacctgc
    15961 tgtacggaaa cgaagggtta ttaccgctcg ataccttcgc ccgctttagc cagaacccgg
    16021 cttaccatac ccaactgtca cagccgatcg aaaccgtgat gctggcgctt aataccgcca
    16081 aagcccccac caatgagctg gcagtacgtg aagctcttaa ttacgcggta aacaaaaaat
    16141 cgctgattga taacgcgttg tatggcaccc agcaggtcgc cgacaccctg tttgcccctt
    16201 ctgtgcccta cgccaatctc ggcctgaaac cgcgccagta cgatccgcag aaagcgaaag
    16261 cgttgctgga aaaagccggt tggacgctgc ctgcgggcaa agacatccgc gagaaaaatg
    16321 gtcagccgct gcgcattgaa ctgtcgttca tcggcaccga tgcgttaagc aaatcgatgg
    16381 cggaaatcat tcaggctgat atgcgccaga ttggcgcaga tgtctcgctg attggcgaag
    16441 aagagagcag tatctatgct cgtcagcgcg acggtcgttt tggcatgatt ttccaccgca
    16501 cctggggcgc gccatacgac ccacacgcct tcctgagttc aatgcgcgta ccgtcacacg
    16561 ctgacttcca ggcacagcaa ggattagccg acaaaccgct gattgataaa gagatcggcg
    16621 aagtgctagc gacccatgac gaaacgcaac gtcaggcgct gtatcgcgac attctgaccc
    16681 gtctgcatga cgaggcggtt tatctgccta tcagttacat ctcaatgatg gtggtatcaa
    16741 aaccggagct gggtaacatc ccctacgcgc cgatcgccac cgaaattccg ttcgaacaga
    16801 ttaaaccggt gaaaccttaa tgttgcgtta cgtattacgc cgctttctgc tgctgatccc
    16861 gatggtgctc gccgcctcgg tgatcatttt tctgatgctg cgccttggta ccggcgaccc
    16921 ggcgctcgat tatttgcgtc tgtctaacct gccgccgacg ccagagatgc tggcctctac
    16981 ccgcaccatg ctcggactgg atcagccgct gtacgtccag tacggtacct ggttgtggaa
    17041 ggcgctgcat cttgactttg gtatctcatt cgccagccaa cgcccagtgc tggacgatat
    17101 gctgaacttc ctgcccgcca cgctggaact tgcaggtgcc gcgctggtat taattctgct
    17161 cacttctgtg ccgctcggta tctgggcggc gcgccatcgc gatcgtctgc cggatttcgc
    17221 cgtacgtttc atcgcgtttc tcggcgtgtc gatgcctaac ttctggctgg cgtttttact
    17281 ggtgatggcg ttttcggtgt atctgcaatg gctacccgcg atgggttacg gcggctggca
    17341 gcacatcatt ttgcctgcgg tttccattgc ctttatgtcg ctggcgatta acgcacggct
    17401 gctgcgcgcc agtatgctgg acgtcgccgg tcagcgtcac gtcacctggg cgcgtctgcg
    17461 cggcctgaac gacaaacaga ccgaacgtcg ccacatcctg cgcaatgcct cgctgccgat
    17521 gatcaccgcc gtggggatgc acattggcga actgattggc gggacgatga ttatcgaaaa
    17581 catctttgcc tggcctggcg tcgggcgcta tgcggtgtcg gcgattttta accgtgacta
    17641 tccggtgatc cagtgcttta cgctgatgat ggtggtggtt tttgtggtct gtaatttgat
    17701 tgtcgatttg ctcaacgccg cgctggaccc gcgcattcgt cgtcatgaag gagcgcacgc
    17761 gtgaactttt tcatctcttc ccgctggtcg gtacgcctgg cgctgatcat tatcgccctg
    17821 ctggcgctga ttgcgctcac cagccagtgg tggctgccgt atgaccccca ggcgattgat
    17881 ttgccgtcgc gcctgctttc gccgaatgcg cagcactggc tgggcaccga tcacttaggt
    17941 cgcgatattt tctcgcggct gatggcagcg acccgcgtgt cgctcggttc ggtaatggcc
    18001 tgcctgctgc tggtgctgac attagggctg gttattggcg gcagcgccgg gctgattggc
    18061 gggcgcgttg atcaggctac catgcgcgtc gccgatatgt ttatgacctt cccgacctcg
    18121 attctgtcgt tctttatggt tggcgtgctc ggcactgggc tgaccaacgt aattatcgcc
    18181 atcgccctgt cgcactgggc gtggtatgca cgcatggtgc gcagcctggt gatttcactg
    18241 cgccagcgcg agtttgtgct ggcatcacgg ctttctggcg cgggccatgt gcgagtgttt
    18301 gttgatcatc tggcaggtgc ggtgatccct tcgctgctgg tgctggcaac gctggatatc
    18361 ggccatatga tgctgcacgt cgcggggatg tccttcctcg gcctcggcgt gaccgcgccg
    18421 accgccgaat ggggcgtgat gattaacgac gcgcgccagt atatctggac ccagccgctg
    18481 caaatgttct ggccggggct ggcgctgttt atcagcgtga tggcctttaa cctggtgggt
    18541 gacgcactgc gcgatcatct ggaccctcat ctggtgacgg agcacgcaca ctaatgccgc
    18601 aacagattga actacgtaat atcgcgctac aggccgcgca gccgctggtg cacggcgtat
    18661 cgttaaccct gaaacgcggg cgtgtgctgg cgttagtcgg cggtagcggc agcgggaaat
    18721 cattaacctg cgccgcggcg ctgggaattc tgcccgcagg cgttcgccag acggcggggg
    18781 aaattttagc cgatggcaaa ccggtttctc cctgcgccct gcgcggcatc aaaattgcca
    18841 ccatcatgca gaacccgcgc agcgccttta atccgctacg caccatgcac acccacgcgc
    18901 gtgaaacctg cctggcgctg gggaaacccg ccgatgacgc tacgcttacc gctgccatag
    18961 aagcggtggg gctggaaaac gccgcacgcg tgctgaagct gtacccgttc gagatgagcg
    19021 gcggcatgtt gcagcgcatg atgattgcga tggcggtgct ttgcgaatca ccgtttatca
    19081 tcgccgatga accaaccacc gacctcgacg tggtagcaca ggcgcgcatc ctcgatctgc
    19141 tggaaagcat tatgcaaaaa caggcaccgg ggatgctgct ggtcacccat gatatgggcg
    19201 tagtggcgcg tctggcggat gacgtggcgg tgatgtcgca cggtaagatt gtcgaacagg
    19261 gcgatgtcga aacgctgttt aacgccccca aacatacggt gacgcgcagc ctggtttccg
    19321 ctcatctcgc cctctacggt atggagctgg catcatgact ttacttaacg tttccggcct
    19381 ttcccatcac tatgcgcacg gtggatttag cggaaaacat cagcaccagg cggtgctgaa
    19441 taacgtttcc ctgaccctga aaagcggcga aaccgtcgcc ctgctggggc gcagcggctg
    19501 cgggaaaagc accctcgcgc ggctgctggt aggcttagag tctccctcac aagggaatat
    19561 tagctggcgc ggcgaatcgt tggcaaagct caatcgcgcc cagcgtaaag cgttccgccg
    19621 cgatattcag atggtatttc aggactccat cagcgccgtg aatccgcgca aaaccgtgcg
    19681 cgagatcctg cgagaaccga tgcgtcacct gctatcactg aaaaaatccg aacaactgga
    19741 gcgcgccagc gaaatgctga aggcggtcga tctcgacgac agcgtgctca acaagcgccc
    19801 accgcagtta agcggcggcc agctccagcg cgtctgcctg gctcgcgcgc tggcggtcga
    19861 accgaaactg ctgattctgg atgaagcagt ttctaacctt gatctcgtgt tacaggcggg
    19921 cgtcattcgc ctgctgaaaa agctacaaca acagtttggc accgcctgcc tgttcatcac
    19981 ccacgactta cgcctggtgg aacgcttttg ccagcgagtg atggtgatgg acaacggaca
    20041 aatcgtcgaa acccaggcgg tgggagataa attaaccttt tcctctgacg ccggacgtgt
    20101 gctacaaaac gcggtattac ccgcattccc cgtgcgccgt cgcacctcag aaaaggttta
    20161 acgcaaatgc aacgagtcac catcacgctt gatgacgatt tactggagac gctggacagc
    20221 ctgagccagc gtcgtggtta taacaaccgt tccgaagcta tccgcgatat tctgcgtagc
    20281 gccctggcgc aggaagctac ccagcagcac ggcacacaag gtttcgcggt gctgtcgtat
    20341 gtgtatgaac acgaaaaacg cgacttagcc agccgtattg tttccaccca gcatcatcac
    20401 cacgacctct ccgtcgccac gctacatgtg cacatcaacc acgacgactg tctggaaatc
    20461 gccgtgttga aaggtgacat gggtgacgtg cagcattttg ccgatgacgt tatcgcccag
    20521 cgcggcgtgc ggcacgggca tttgcagtgc ttgccgaagg aagattgagt ctattattgg
    20581 gtcctttggt gaacgatcaa caaagggcca ctagcacacc tgattcctct ctaaataccc
    20641 tatccgaacc ttctcttttg taacgttcta aatatattcc taaaaatctt caattcattg
    20701 tgaccacaag tttttcttcg ctttttcgta tgaagatact gtcattaaaa taata
//
LOCUS       DAZGCQ010000088        20255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000088 DAZGCQ010000000
VERSION     DAZGCQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.088"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..1510
                     /locus_tag="ACHK07_003614"
     CDS             56..1510
                     /locus_tag="ACHK07_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO8103898.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1570..2931
                     /gene="allB"
                     /locus_tag="ACHK07_003615"
     CDS             1570..2931
                     /gene="allB"
                     /locus_tag="ACHK07_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO8103899.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2988..4289
                     /locus_tag="ACHK07_003616"
     CDS             2988..4289
                     /locus_tag="ACHK07_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO8103900.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4311..5456
                     /gene="glxK"
                     /locus_tag="ACHK07_003617"
     CDS             4311..5456
                     /gene="glxK"
                     /locus_tag="ACHK07_003617"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO8103901.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEACGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5585..6370)
                     /gene="allE"
                     /locus_tag="ACHK07_003618"
     CDS             complement(5585..6370)
                     /gene="allE"
                     /locus_tag="ACHK07_003618"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO8103902.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6381..7616)
                     /gene="allC"
                     /locus_tag="ACHK07_003619"
     CDS             complement(6381..7616)
                     /gene="allC"
                     /locus_tag="ACHK07_003619"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO8103903.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLETQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPHVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7638..8687)
                     /gene="allD"
                     /locus_tag="ACHK07_003620"
     CDS             complement(7638..8687)
                     /gene="allD"
                     /locus_tag="ACHK07_003620"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO8103904.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            9004..10671
                     /locus_tag="ACHK07_003621"
     CDS             9004..10671
                     /locus_tag="ACHK07_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8103905.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFRHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALCLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGNLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10681..11940
                     /locus_tag="ACHK07_003622"
     CDS             10681..11940
                     /locus_tag="ACHK07_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8103906.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11951..12766
                     /locus_tag="ACHK07_003623"
     CDS             11951..12766
                     /locus_tag="ACHK07_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8103907.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVTQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12763..13656
                     /locus_tag="ACHK07_003624"
     CDS             12763..13656
                     /locus_tag="ACHK07_003624"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8103908.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13795..14862)
                     /gene="purK"
                     /locus_tag="ACHK07_003625"
     CDS             complement(13795..14862)
                     /gene="purK"
                     /locus_tag="ACHK07_003625"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO8103909.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14859..15368)
                     /gene="purE"
                     /locus_tag="ACHK07_003626"
     CDS             complement(14859..15368)
                     /gene="purE"
                     /locus_tag="ACHK07_003626"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO8103910.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15486..16208)
                     /gene="lpxH"
                     /locus_tag="ACHK07_003627"
     CDS             complement(15486..16208)
                     /gene="lpxH"
                     /locus_tag="ACHK07_003627"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO8103911.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16211..16705)
                     /gene="ppiB"
                     /locus_tag="ACHK07_003628"
     CDS             complement(16211..16705)
                     /gene="ppiB"
                     /locus_tag="ACHK07_003628"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO8103912.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16879..18264
                     /gene="cysS"
                     /locus_tag="ACHK07_003629"
     CDS             16879..18264
                     /gene="cysS"
                     /locus_tag="ACHK07_003629"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO8103913.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18300..18821)
                     /locus_tag="ACHK07_003630"
     CDS             complement(18300..18821)
                     /locus_tag="ACHK07_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8103914.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(18929..19141)
                     /gene="ybcJ"
                     /locus_tag="ACHK07_003631"
     CDS             complement(18929..19141)
                     /gene="ybcJ"
                     /locus_tag="ACHK07_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO8103915.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19143..20009)
                     /gene="folD"
                     /locus_tag="ACHK07_003632"
     CDS             complement(19143..20009)
                     /gene="folD"
                     /locus_tag="ACHK07_003632"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO8103916.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         4625 a         5154 c         5571 g         4905 t
ORIGIN      
        1 tcctcatagc tagattaaaa caacgttatt cgatacgtga aattaagagg gatttatgga
       61 acatcagaga aaactattcc agcaacgcgg ctatagcgaa gatctattgc cgaaaacgca
      121 aagccagcgg acctggaaaa catttaacta ttttacctta tggatgggtt cggttcataa
      181 cgttcccaat tatgtgatgg tcggcggctt ttttattctc ggcttgtcta cctttagtat
      241 tatgctggca attatcctca gcgccttttt cattgccgcg gtaatggtat taaacggcgc
      301 tgcgggcagt aaatacggcg taccgtttgc catgatcctg cgtgcttctt acggcgtacg
      361 tggcgcactg tttcccggat tattaagagg cggtattgct gccattatgt ggtttggcct
      421 gcaatgttac gcggggtcac tggcctgctt gattctgatt ggcaaaatct ggccgggatt
      481 tttgactctc ggtggtgatt tcactctgtt aggcctttct ctaccgggct taattacttt
      541 cttactcttc tggctggtca acgttggtat aggttttggc ggtggcaaag ttttaaataa
      601 attcactgcc attcttaacc cgtgcatcta tatcgttttc ggcggtatgg cgatttgggc
      661 gatttcgctg gtcgggctcg gtccaatctt tgactacatt ccgagcggta ttcagaaagc
      721 agaaaacagt ggcttcctgt tcctggtggt gattaacgcg gtagttgcgg tctgggcggc
      781 tccggcggtg agcgcatccg actttacgca aaacgcccac tcgtttcgtg agcaagcgct
      841 ggggcaaacg ctgggtttag ttgtggccta tattctgttt gcggtcgccg gggtatgtat
      901 tattgccgga gccagtattc attacggcgc tgatacctgg aacgtgctgg atattgttca
      961 gcgttgggac agcctgtttg cctcgttctt tgcggtactg gttattctga tgacgactat
     1021 ctccactaac gccactggta atattattcc ggctggttat cagattgctg ccattgcacc
     1081 gacaaaactg acctataaaa acggcgtact gattgccagt attatcagcc tgctgatctg
     1141 cccgtggaaa ttaatggaaa atcaggacag tatttatctg ttcctcgata ttatcggcgg
     1201 aatgcttggt ccggtaattg gtgtcatgat ggcgcattat tttgtggtga tgcgtgggca
     1261 aattaatctt gatgaactgt acaccgcacc tggcgattat aaatattacg ataacggttt
     1321 taacctcacc gcgttttcag taactctggt ggccgttatt ttatctcttg gcggtaagtt
     1381 tattcccttt atggaaccgt tatcgcgtgt ctcatggttt gtcggcgtca tcgtcgcctt
     1441 tgcggcctac gccttattaa agaaacgtac aacagcagaa aaaacaggag agcaaaaaac
     1501 cataggttaa ttaatcccga tattgaacat tgagttaaaa accaatctgt attttacaag
     1561 gagtttgtta tgtcttttga tttaatcatt aaaaacggca ccgttatttt agaaaacgaa
     1621 gctcgcgttg tagatatcgc cgttaaaggc ggcaaaattg ctgctatcgg tcaggatctg
     1681 ggcgatgcaa aagaagttat ggatgcgtct ggtctggtgg tttcgccggg catggttgat
     1741 gcgcacaccc atatttctga accgggtcgt agccactggg aaggttatga aaccggtact
     1801 cgcgcagcgg caaaaggtgg tatcaccacc atgatcgaaa tgccgctcaa ccagctgcct
     1861 gcaacggttg accgcgcttc aattgaactg aagttcgatg ccgctaaagg caagctgact
     1921 atcgatgcgg cacaactcgg tggcctggtg tcttacaaca tcgaccgtct gcatgagctg
     1981 gatgaagtgg gcgttgtcgg cttcaaatgc ttcgttgcga cctgtggcga tcgcggtatc
     2041 gacaacgact tccgtgatgt aaatgactgg cagttcttca aaggcgcgca gaagctgggc
     2101 gaactggggc agccggtgct ggtgcactgc gaaaacgcgc tgatctgtga cgcactgggc
     2161 gaagaagcga aacgtgaagg tcgcgtaact gcccatgact atgtggcttc gcgtccggta
     2221 tttaccgaag tggaagcgat tcgccgcgtg ctgtatctgg cgaaagttgc cggttgccgt
     2281 ctgcacgttt gccatatcag cagcccggaa ggcgttgaag aagtgactcg tgcacgtcag
     2341 gaaggtcagg atgttacttg tgaatcctgc ccgcattact ttgtactgga taccgatcag
     2401 ttcgaagaaa tcggcactct ggcgaagtgt tcaccgccga tccgcgatct ggaaaaccag
     2461 aaaggcatgt gggaaaaact gtttaacggt gaaatcgact gcctggtttc cgaccactca
     2521 ccatgccctc cggaaatgaa agccggcaac atcatggaag catggggcgg tattgccggt
     2581 ctgcaaaact gtatggacgt gatgttcgat gaagcggtac agaaacgcgg aatgtctctg
     2641 ccaatgttcg gcaaattaat ggcgactaac gcagcagata ttttcggtct gcagcaaaaa
     2701 ggccgtatcg ccccaggaaa agatgccgac ttcgtcttca ttcagccgaa tagcagctat
     2761 gttcttacca atgacgatct ggaatatcgc cacaaagtca gcccgtatgt tggccgtacc
     2821 attggcgcgc gtatcacgaa aaccatctta cgtggtgatg tgatttacga catcgaacag
     2881 ggcttccctg ttgcgccgaa aggtcaattt atccttaaac atcagcagta atctggcccc
     2941 tgcaatgccc gtccttgcgg cgggcattct ccggttaagg tgtgtttatg ttcaattttg
     3001 cagtcagccg cgaaagcctg ttatcaggat ttcagtggtt tttctttatt ttttgcaaca
     3061 cggttgtggt tcctcctacg ctactttctg cttttcagtt gccgcaaagt agcctgctta
     3121 cactcacaca atatgctttt cttgctaccg cactggcctg cttcgctcag gcgttctgtg
     3181 gccatcgtcg cgctattatg gaagggccgg ggggcctgtg gtggggaacc atcctaacta
     3241 tcacccttgg cgaagcatcg cgcgggacac cgatcaacga tatcgccacc agcctggcag
     3301 tggggattgc gctctccggc gtgctgacga tgttgattgg ttttagcgga ttaggccatc
     3361 gcctggcacg gttatttacg ccgtcggtga tggtcttgtt tatgttgatg ctgggcgcac
     3421 agctgaccac tatctttttc aaaggtatgc tcgggctgcc gtttggcata gccgacccaa
     3481 attttaaaat tcagttacca ccattcgcgc tctcggtggc ggtgatgtgc ctggtactgg
     3541 cgatgattat ctttctgccg caacgttttg cccgttatgg cctgctggtc ggcaccataa
     3601 ccggctggtt gttgtggtac ttttgctttc cttcttcgca ttcgctctcc ggtgagttgc
     3661 actggcagtg gttcccgctc ggcagtggcg gtgctttgtc gccgggaatt attctgacgg
     3721 cggtgattac aggtctggta aatatcagca atacctacgg tgcgattcgg ggcacggatg
     3781 ttttttatcc gcagcagggc gcggggaata cgcgttatcg tcgtagcttt gtggcgaccg
     3841 gatttatgac actgataacc gtaccgctgg cggtaattcc attttcaccg tttgtttcat
     3901 ccattggttt attaacccag actggcgatt acacgcggcg ttcgtttatt tatggcagcg
     3961 ttatttgcct gctggtggcg ctggttccgg cactcacgcg actgttttgc agtatccctt
     4021 tacccgtgag tagtgcggtc atgctggttt cttatctgcc tttactcttt tccgcgctgg
     4081 tgtttagcca gcaaataacg tttaccgctc gcaatattta tcgactcgca ttgccgttat
     4141 ttgtcggcat atttttaatg gcattaccgc ctgtgtatct gcaagacctt ccattaacgc
     4201 ttcgtcctct gctcagtaac ggcttattgg tcgggatttt actggctgtt cttatggata
     4261 accttattcc gtgggaacgc atcgaataat ttgttgaaaa aggattgata atgaagattg
     4321 tcattgcgcc agactctttt aaagagagct taagtgcaga aaaatgttgt caggcaatta
     4381 aagccgggtt ttcgaccctc tttcccgatg cgaactatat ctgtttgccg atagcggatg
     4441 gcggcgaagg gacggtggat gcgatggtcg ccgcgacggg tggtaacatc gtgacgcttg
     4501 aagcctgcgg gccgatgggc gaaaaagtga atgcttttta tggccttacc ggcgacggga
     4561 aaacagcggt gattgagatg gcggcagcaa gtggcctgat gctggtcgcg cctgaaaagc
     4621 gtaatccgtt gctggcctcc agttttggta cgggggagtt aattcggcat gcgctggata
     4681 acgacattcg ccatattatt ctcggcattg gcggcagtgc gacggtcgac ggcggtatgg
     4741 gcatggcgca ggcgcttggt gtgcgttttc tcgatgccga cggtcaggtg ctggcagcta
     4801 acggcggcaa tttagcgcgc gtggcaagca ttgagatgga ggaatgcgat ccgcgtctgg
     4861 cgaactgcca tattgaagta gcatgtgacg ttgataaccc gctggtaggg gcacgcggcg
     4921 cggcggcggt gtttggcccg caaaaagggg caacgccgga gatggtcgaa gagctggaac
     4981 aggggctgca aaattacgcc cgtgttttac aacagcaaac tgaaattaat gtctgtcaga
     5041 tggcgggcgg cggcgctgcg ggcggtatgg gtattgcggc ggcggtattt ctcaatgcgg
     5101 atattaaacc gggtattgag attgtactgc gtgcggtcaa cctggagcag gcggtgcagg
     5161 gggcagcact ggtgatcacc ggggaagggc gcattgactc gcaaacggca ggcggtaaag
     5221 cgccgctggg cgttgcgtcg gtggcgaagc agtttaatgt accggtgatt gggattgctg
     5281 gcgtattggg tgatggcgtg gaagtggtgc accagtacgg cattgacgcg gtattcagca
     5341 ttttgcctcg tctggcacct ttagccgaag tgctcgccag cggtgaaacc aatctcttca
     5401 acagcgcgcg aaatattgcc tgcgccatta aaataggtca gggaattaaa aactgaccct
     5461 tacctttaaa gcggatacga tttatatcgc atcttttttt acctgaattt actacaaaaa
     5521 aagccacggt ataaaccgtg gcaaaatcca acatagctaa aaataatcag gcgagtggta
     5581 tgacttaaat ctctacgtcg cggttgcaat ctttcgagta aatatagctg aacgcttcac
     5641 cacgccctac gccataacca gcctgtaaag aataagcgcc cataaagatg taatcgcctt
     5701 ttttcaccgg gatccagtta ttgtcgaggt tataaacccc ctgaccggaa agaatatagg
     5761 caccgtgttc ctgaacgtgt gtttcgatat aaccatggct ggcacctggt gcaaaagaga
     5821 ggatatgcat gttcatatca aaacctaact ctttgggcag aaaatccagc aggatcacat
     5881 catccatacc ttcataatga atgcgttcca gttcgctggc attgccagaa accagccacg
     5941 gtgcatggcc ttctaccgga acatagcggc gcttatataa aaagatttgg ctgtcttcgg
     6001 cctgggcgtt aacaaacgtc attaaggagc ctggcgggca ataaagatag ccaccttcgc
     6061 ttaaggcaaa tgttttgcct tcggctttgg cagtgatatt tccagagatc acatacagga
     6121 acgtttcaat gccttcgcca ccgaagccct gttggttgcc accgttttga tgcagtgtga
     6181 ccagataatc aacaaaagag gcacccagct ttggcgtgga gaggattgtc gcgtcacaat
     6241 tttcaaagcc cggaataata ttttttacca gaccgtctgg tgttaacagt gcgaaattac
     6301 cgtgtttcac aatcgcacgg ttagccagta aatcatcgcg gtaaccggtg acgttattta
     6361 aatatcccat ttatgactcc ttatttctgc caggcaagtt gataaagcat gagtgccaac
     6421 gttttgaccc cttcggcaag gtcggtaata ttggtgcgtt ccgccgggtt atggctgatc
     6481 ccgttgatgc tgggaatgaa aatcatgcag gtcggcacgt gaggcgcgaa aatctgcgcg
     6541 tcgtgcccgg caccactgtg catcacccgg taattcagtt tttcactttc acacaattct
     6601 gtcagggtgg cgaccaggtc cttattcatc ggcacgggtt cttcgtccat ccataaatcg
     6661 atatcaatac caatgtccat ttcatcgcaa atcgcccgca tgtcgttttc taactgctga
     6721 gtgaaatcgc gcagcacggc ggcgtcggta tgacgacaat caatggtgaa tgtggtttta
     6781 cccggcacca cattcaccgt attcgggcgc ggctccacct taccaaaggt cagcaccagc
     6841 gggtcgccca tctttttcgc tttttcgatc gactgatggc aaatgcgact gaaagcgtaa
     6901 accgtatcac gacgataacc catcggcgtg gtgcctgcat ggtttgattc gccgttcagc
     6961 gtcaccgtat agcgacgttg cccgacaatt gcattcacca cgccaattga ttgcccatta
     7021 ctttccagca cacagccctg ttcgatatgc agttcgacaa aggctttaat atcctgacgc
     7081 ggagttagcg gggcgtccgg cagagtaaat ccgcaagcct tcatcgcatc gacaaaacta
     7141 tttcctttgg catcacagat attccgcacg tcgtcaggat tcgccagccc aaagatattt
     7201 ttactgcccc agaaaacata cgggaagcgg ctgccttctt cttccgccat cgccaccact
     7261 tcaaccgtac gcagcggtgc gccgtattgc gtttccagcc agtcaattgc cagccacgcc
     7321 gccagcgcgc cgaattgccc gtcgaggtta ccgccgttaa ccacggtatc gatatgcgaa
     7381 ccgctcagaa ccacttcctg tggatattcg gtgccactca ggcgaccgta taaattcccc
     7441 acttcatcga aacgtgtttc cagcccgctt gctgccattc tttttttaaa ttgctgctgg
     7501 gtttccagcc attccggtga ataaagtaaa cgggtcatcc cacccgttgg gtcagcgcca
     7561 aatgaggaaa gccagggtag cgtttcttct atggcttgac gaaaatgtgt aatcataaga
     7621 aagtcctgtc tcaataatta ttgcgcaaag ggatttttcg tttcgtatga cgtgttataa
     7681 agcgcgtcgg aaattaaata ctggtaaata tcatcaacaa tttcgatgcc ttcgacggcg
     7741 gctttgcgtt gtttaatatc ctgatcctgt ccgggataat aaacctgatt aaaaccgggc
     7801 gcgggggtaa tggcatttaa ttcgcgcatg gtctggctaa gatgttgacg gaataattcg
     7861 ctggaggaga aaaagttcgg gttaataact acatgtaatt gccccaaatt acgcccggcg
     7921 tgtaaatcgt catacatcga actaacctgt cgcccgaacg gtaagccgag taagacgcct
     7981 gagaggacgt caatcatcat catcaggcca taccctttcg gcccggctgc ggggagcaga
     8041 gcatgtaccg cgaacggatc ggttgttggt gcaccgtttt tatcgaccgc ccaggtatcc
     8101 gggatagaca tattacgcga gcgggcgtcg agcacttttc cccatgcctg tacggtagtc
     8161 gccatatcaa aggtaaggat ctcgtcgcct tctcccggcg cggcaaaggc cagtgggtta
     8221 gtaccgtagt aaatttccgc gccgccaaaa ggcaccacca ttggatcgga ctggcacatc
     8281 gaaatgccaa ttaatccggc gcgggctgcc tgctgcacaa aataagagat tgcgccgctg
     8341 tgacccatcc ggctgatacc gaccaccgca acgccatttt gctgggcggt tttgatggca
     8401 tgttccatac ccattttagc cgcgacctgt ccggcggcat tgtcggcatg taaaattgcc
     8461 gagcacggcc cggtttcctc aagacgaaac tccggttcgc ggttggtgcc gccttttgaa
     8521 atgcgttccg cgtagtattc cacgcgcacc gcaccatgag agtggatccc tctggcatcg
     8581 gcgtaaacca atacttcagc cacggttgca gcgtgctcac gttttaaccc agcctggcag
     8641 agtttattct caattagctg gtggagtgtt tcccgactga ttttcatctg tcttcctttt
     8701 taacgacggt gtgaagcatg actgcaatta acatacaggg aaaatatctg gattatgtga
     8761 tccagacagg caaaaaaata tagttagaat ttatttgata atccgctcgc ttttaacctg
     8821 gtttttaaaa caaccacgct tattaaaaaa taacacgtaa tagcatggcg gttatttgaa
     8881 ttcttttgtt aataatccct gtgtgatatt catcacctta tttacccgtt gtcatcgata
     8941 ccgtaatcgc cacattaaca ctgctcgtgc aattgccatg ggtgcaattt ttaaggagtt
     9001 gttatgatcc acgcctttat taaaaaaggg tgttttcagg attcggtcag tttaatgatt
     9061 atttcacgaa aactcagcga atcagaaaat gttgatgatg tttccgtaat gatgggtacg
     9121 cccgccaata aagcgttatt agataccaca ggtttcaggc atgacgattt taataacgcc
     9181 acgccgaacg atatttgcgt ggcaattcgt agcgaagcgg cggatgcggg gatcgcgcag
     9241 gcgattatgc agcagcttga agaggcgcta aaacaactgg cgcagggggc aggcagcagc
     9301 caggcgttga cgcaggtgcg tcgctgggac agtgcctgtc agaaattacc cgatgccaat
     9361 ctggcgctga tttcagtggc tggcgagtat gcggcggagc tggcgaatca ggcgctggat
     9421 cgcaacctca acgtgatgat gttctccgat aacgtcacgc tggaagatga aatccaactt
     9481 aaaacccgcg cgcgggaaaa aggcttgctg gtgatggggc cggactgcgg tacgtcgatg
     9541 attgccggca cgccgctggc ttttgctaac gtgatgccgg aaggcaacat tggcgtcatt
     9601 ggcgcttccg gtaccgggat tcaggagctg tgctcgcaga ttgcgctggc aggggaggga
     9661 attactcacg cgattggcct tggcggccgc gacctcagcc gtgaagtggg cggcatcagt
     9721 gcgctgacgg cgctggaaat gctcagtgca gacgagaaaa gcgaagtgct ggcatttgtt
     9781 tcaaaaccac ctgccgaagc ggtgcgtctg aaaattgtta atgccatgaa agcaaccggc
     9841 aaaccgacgg tggcgctgtg tttaggttat accccggcgg tggcccgcga cgagaatgtc
     9901 tggtttgcct cctcgctgga tgaggccgca cgcctggctt gcctgctttc acgcgttacg
     9961 gcgcgacgta acgcgataac gcctgccagc agcggattta tttgcggttt gtataccggc
    10021 ggtacgctgg ctgccgaagc ggcgggatta cttgccggaa accttggcgt ggaagccgat
    10081 gatgcccatc atcacggcat gatgctggac gccgatggcc accagattat tgacctcggc
    10141 gatgatttct acaccgtcgg gcgtccccat ccgatgatcg acccaacctt acgcaaccag
    10201 ttaattgccg atctcggcgc taaaccgcaa gtgcgtgtgc tgctgcttga tgtcgtgatt
    10261 ggcttcggtg cgactgccga tcctgccgcc tcgctggtaa gcgcctggca aaaagcctgt
    10321 gctgcgcgtt cagacaatca accactgtat gccattgcca cggtgacagg cactgaacgt
    10381 gacccgcaat gccgctcgca gcaaatcgcc acgctggaag atgcggggat tgcggtcgtg
    10441 agttcgctac cggaagccac cttgctggcg gcagcgttaa ttcatccgct ctcgcctgcc
    10501 gcacagcaac acacaccgtc attattggaa aacgtcgccg tgattaacat cggattacgc
    10561 agttttgcgc tggagctaca aagcgccagc aaaccggttg tgcattacca atggtcgcca
    10621 gtcgccggtg gcaataaaaa actggctcgt ttattagaac gtttgcaata aggggttccc
    10681 atgtttacat cagtggcgca agccaatgct gcggttatcg aacaaattcg tcgcgctcgt
    10741 ccacactggc tggatgtgca accggcttct tcacttatca gcgaactaaa ccagggcaaa
    10801 acactgcttc acgccgggcc gccaatgcgc tggcaggaga tgaccggacc catgaaaggg
    10861 gcgtgcgtgg gcgcatgtct gttcgaaggc tgggcgaaag atgaagcgca ggcgctggca
    10921 atactggagc agggggaagt gaacttcatt ccttgtcacc atgtgaatgc cgtcgggcca
    10981 atgggcggta ttacttctgc cagtatgccg atgctggtgg tcgagaacgt gaccgacggc
    11041 aaccgggcgt actgcaacct caacgaaggt atcggcaaag tgatgcgttt tggcgcttac
    11101 ggcgaagatg tcctgactcg ccatcgctgg atgcgcgatg tgttaatgcc agtattaagc
    11161 gcggcgctgg ggcgcatgga acgcggtatc gatcttaccg cgatgatggc gcagggcatt
    11221 acgatgggcg atgagttcca tcaacgcaat attgcttcct cagcactgtt aatgcgtgcg
    11281 ctggccccac aaattgctcg cctcgatcat gataaacagc acatcgcgga agtgatggat
    11341 ttcctcagcg tgaccgatca gttcttcctc aacctcgcga tggcttactg caaggcggcg
    11401 atggatgctg gcgcgatgat ccgcgcaggc agcatcgtca cggcaatgac ccgcaacggc
    11461 aatatgttcg ggattcgggt aagcgggctg ggcgaacgct ggtttactgc gcctgtaaac
    11521 actccgcaag gtctgttttt caccggcttc tcgcaggagc aggcgaaccc ggatatgggc
    11581 gatagcgcga ttaccgaaac ctttggtatc ggaggtgcgg caatgatcgc agcgcctggc
    11641 gtaacgcgct ttgtcggtgc gggtggcatg gaagcggcca gggcggtatc tgaagagatg
    11701 gcggaaattt accttgaacg caatatgcag ttgcagatcc caagctggga ttttcagggc
    11761 gcgtgcctgg gactggacat tcgtcgcgtg gtagaaaccg gcattacgcc actcatcaat
    11821 accggtatcg cccataaaga ggcggggatc gggcagattg gcgcaggcac cgtgcgggca
    11881 ccgctggcgt gctttgaaca ggcgctggaa gcactggctg aaagcatggg tattggttga
    11941 ggaacgcgca atgacgatca tccatcctct gcttgccagt agtagcgcac cgaattatcg
    12001 ccagtcctgg cggttagcgg gagtgtggcg gcgggcgatt aacctgatga cggaaagcgg
    12061 cgaactgtta acgctgcatc gtcagggtag tggtttcggc cccggaggat gggtgcttcg
    12121 ccgtgcgcaa ttcgatgcgt tatgcggtgg attatgcggc aatgaacgac cacaggttgt
    12181 ggctcaaggg attcgcctcg ggcgtttcac ggttacacag ccacagcgtt attgtttgct
    12241 gcgtattacg ccgccttcgc atcctcaacc gcttgcagct gcatggatgc aacgcgcgga
    12301 ggaaaccgga cttttcgggc cactggcgat ggcggcaagc gatccgctgc ccgctgaatt
    12361 acgccagttt cgtcactgtt ttcaggccgc gctcaatggc gttaagaccg actggcggca
    12421 ctggctgggt aaaggccccg gattaacgcc aagtcatgat gacacgttga gcggaatgct
    12481 gctggcggcc tggtattatg gcgctttaga tgcgcgctcc ggtcgtccgt tttttgcctg
    12541 ttccgacaat ctgcaactcg ttaccacagc ggtgagcgtc agttatttac gttatgccgc
    12601 gcaaggatat ttcgcctcgc cactcctgca ctttgttcat gctctgagtt gcccgaaacg
    12661 taccgctgtt gcgattgatt cgctgctggc gctggggcat acgtcagggg cagatacact
    12721 gctggggttc tggcttggtc aacaattatt acaaggaaaa ccatgaaaac actggttgta
    12781 gctcttgggg gcaatgcctt gcttcagcgc ggtgaggcgc tgacggcaga aaatcaatat
    12841 cgcaatatcg ccagtgctgt acccgcgctg gcgcgcctag cccgttctta tcggctggcg
    12901 attgttcacg gcaacgggcc gcaggtgggg ctgctggcgt tacagaatct ggcgtggaaa
    12961 gaggtagaac cgtatccgct ggatgtgctg gtggcggaaa gccaggggat gattggctat
    13021 atgctggcgc agagtttgag cgcacagccg cagatgccgc ctgtcacgac ggtgctgacg
    13081 cgcattgagg tttcgcctga tgatccggcg tttttgcagc cagagaaatt tattggtccg
    13141 gtttatcagc cagaagaaca agaggcactg gaagcggctt acggctggca gatgaaacgt
    13201 gatggtaaat atttgcgccg ggtggtggcg tctccgcaac cgcgtaaaat tctcgacagc
    13261 gaagccatcg agttgttgct caaagagggg catgtggtga tttgcagtgg cggcggcggt
    13321 gtgcctgtga cggaagacgg ggcagggagt gaagcggtga ttgataaaga tctcgctgct
    13381 gcgttgctcg ccgagcagat taatgccgat ggactggtga tcctcaccga tgctgatgcg
    13441 gtatatgaaa actggggaac gccgcagcaa cgtgccattc gccatgccac accggatgag
    13501 ttagcgccat ttgccaaagc cgatggttcg atggggccga aggtaacggc ggtgagtggt
    13561 tatgtcagaa gccgtggtaa acccgcgtgg attggggcgt tatcgcgaat tgaagagacg
    13621 ctggcgggcg aagcggggac ctgtatttcg ctgtagtgat gagagcatcc agcaatcggt
    13681 gcaccatgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaatggg taccggagcg
    13741 gtgtaggccg gataaggcgt tcacgccgca tccggcaatc aaatgagcac caggttaact
    13801 gaacttactc tgtgcccaca tcacgccgct ggcatactcc ggcggcagca gcgggattaa
    13861 ggcttccagc gtcgcggaca gacgcgatgt gtcgctgtcg gtcaaattca gatgccccac
    13921 tttacggccc ggacggactt ctttgtcgta ccagtgcaga tgcaccagcg gcagtttcag
    13981 ccagtcataa ttcacatcgc taccaatcag attgatcatc accgacggat tattcaccac
    14041 tggttgcggt aacggcagat cggtaatcgc ccgcagatgc agctcaaact ggctgatgct
    14101 ggcaccgttt tgtgtccagt gaccgctgtt atgcacacgc ggagccagtt cgttgatcag
    14161 cagaccttgc ggggtgacaa aacactccat cgccatcacg cccacatagc ccagctcctg
    14221 cataatcgcc gacagcatct cttcggcttg cgcttgctgc tgtgcattgg cctgcgggaa
    14281 agcgacgctg gtgcgcaaaa taccgtcctg atgcaggtta tgcgtcagcg gataaaacac
    14341 ggtgctgcca tcaaagccgc gcgcgccaac cagcgacact tcaccagaga agtttatgcc
    14401 ctgctcgaca atacattcgc cgtagcattc agccggtaac tgctcggttt catcggcgcg
    14461 taaacgccat tgaccgcgtc cgtcatagcc accagtgcga cgcttaacaa tcgccagctc
    14521 acctaaacga tcaaacaccg caggccactc gctgcgatcg gcaagtaact gccacggtgc
    14581 ggtcggcagg tggagcttat cgaaaagctg cttctgagtc agacggtcag caataatcgg
    14641 gaacacatcg cggttcacaa aggcaggatg gcgcgccagc tcgcgggtta atgcggtttc
    14701 cggccagcgt tcgatctcag cggtaatcac gctttgttga aaaggcaccg ccgccggttc
    14761 agcgtccagc ccgactggcc agacagcaat gcctaacggt tcgcctgcct gacgcagcat
    14821 acggcctaac tgcccgttcc cgaggacgca aacctgtttc atgccgcacc tcgcgggtcc
    14881 gggttttcca gcacttcgtc ggtctgggct ttgcgccagt cattcagacg ctggtgcagt
    14941 tctttatcat gagtcgcaag aatttgtgct gccagtaacg ccgcgtttgc cgcgccagct
    15001 ttaccaatcg ccagcgtacc caccggaatg ccgcgcggca tttgtacgat ggagtagagg
    15061 ctatcgacac cgctcagtgc ggcgctctgt actggcacgc ccagcactgg caccagcgtt
    15121 ttggcggcaa tcatgcctgg caggtgcgct gcgccgcctg cgcccgcaat aatcacctga
    15181 taaccgttct cttcggcact ttcggcgaag ctgaatagtt tatcgggggt gcggtgagca
    15241 gaaaccactt caacgtggtg cgggacattc aggatttcga agatttcggc ggcgaactgc
    15301 atggtagccc agtcgctttt ggaccccatc acgatggcga cacgcgccgg attattgcgg
    15361 gaagacatgc gtcttaaaac tcctgtggtg cacaactctc ggctttagag ggcacagaga
    15421 atagcacgga aagagagcaa ggaaaacggt tgcgtggctg tgaaatcagc aaagttgcgg
    15481 gttttttaaa acggaaaatg aatcagctca acgtcatccg ccgtgacttt caccatcgaa
    15541 ccttccgtat gccaggcacc gagtaccacg cgaaaagcgg gttgctggtt ggcgataagt
    15601 tcatgtaccg ccgggcgatg ggtatgcccg tggatcagcc attgcacctg atgtttttcc
    15661 atcgcactga ccaccgcgtt ttggttaacg tccatgatcg ccagcgattt actgctgttg
    15721 gcttctttac tgttcgcgcg cattcgcgcg gcaatgcgtt tgcgcacaaa caacggcagg
    15781 gcgaggaata gcgtttgcag ccagggtttg tggactttgg cgcgaaaagc ctgataaccc
    15841 gcgtcatcgg tgcacagcgt gtcgccatgc ataatcaaca cccgacgacc ataaagttcg
    15901 agcacctttt cttccggcaa taacgtcatg ccgctttcac gggcaaagcg tttgccgagc
    15961 agaaaatcac ggttgccatg aatgaaataa cagggaacgc cggaatcgga caccgctttg
    16021 atcgctgccg ccatctggcg atggagtggg ttgggatcgt cgtcgccaat ccacgcttca
    16081 aacagatcgc caagaatata cagcgcgtcg gcattgcggg cttcccccgc taaaaaacgc
    16141 agaaaaccgg cggtgatcgc cggttcttcc acgcagagat gaagatctgc aataaagagt
    16201 gtcgccacga ttactcgcta acggtcacgc tttcaatgat aacgtcttct tttggcacgt
    16261 cctggtgcat accgctacga ccggttgcaa cacctttgat tttgtctacc acgtccatgc
    16321 cttcaaccac ttcagcaaac acgcagtagc cccaaccttg caggctttcg ccagagaagt
    16381 tcaggaagtc gttatcaacc acgttgatga agaactgtgc agttgcagag tgcggagcct
    16441 gagtacgggc cattgccagc gtaccacggg tatttttcag accgttgttg gcttcgtttt
    16501 tgatcggttc tttggtggct ttttgtttca tgcccggttc aaaaccaccg ccctgaatca
    16561 taaagccgtt gataacacgg tggaaaatgg tgttgttgta aaaaccttcg cggcagtagt
    16621 ccaggaagtt tttaactgtt tcaggtgctt tatcgtcaaa agttttgatg acaatatcgc
    16681 cgtgattggt gtggaaagta accatttttg catcctgttc cgtttgattg gtgcttcaac
    16741 ccagttcggg tcatatatag ggtggtgtta tagcataacc gcacgatcgg atcatcacgc
    16801 aatgtatgct gattcgcgcg ggaaatatgg gtattatacg caactcaatt acccacacat
    16861 gtctaaacgg aatcttcgat gctaaaaatc ttcaatactc tgacacgcca aaaagaggaa
    16921 tttaagccta ttcacgccgg ggaagtcggc atgtacgtgt gtggaatcac cgtttacgat
    16981 ctctgtcata tcggtcacgg gcgtaccttt gttgcttttg acgtggttgc gcgctatctg
    17041 cgtttcctcg gctataagct gaagtatgtg cgcaacatta ccgatatcga cgacaaaatc
    17101 atcaaacgcg ccaatgaaaa tggcgaaagc tttgtggcgc tggtggatcg catgatcgcc
    17161 gaaatgcaca aagattttga tgcgttgaac attctgcgcc cggatatgga gccgcgcgcg
    17221 acgcaccata tcgcagaaat tattgaactc actgaacaac tgatcgccaa aggtcacgct
    17281 tatgtggcgg acaacggcga cgtgatgttc gacgtcccga ccgatccaac ttatggcgtg
    17341 ctgtcgcgtc aggatctcga ccagctgcag gcaggcgcgc gcgttgacgt ggtcgacgac
    17401 aaacgcaacc cgatggactt cgttctgtgg aagatgtcga aagagggcga accgagctgg
    17461 ccgtctccgt ggggcgcggg ccgtcctggc tggcacattg aatgttcggc aatgaactgc
    17521 aagcagctgg gtaaccactt tgatatccac ggcggcggtt ctgacctgat gttcccacac
    17581 cacgaaaacg aaatcgcgca gtccacctgt gcccatgatg gccagtatgt gaactactgg
    17641 atgcactcgg ggatggtgat ggttgaccgc gagaagatgt ccaaatcgct gggtaacttc
    17701 tttaccgtgc gcgatgtgct gaaatactac gacgcggaaa ccgtgcgtta cttcctgatg
    17761 tcgggccact atcgcagcca gctgaactat agcgaagaga acctgaagca ggcgcgtgcg
    17821 gcgctggagc gtctctacac tgcgctgcgc ggcacagata aaaccgttgc gcctgccggt
    17881 ggcgaagcgt ttgaagcgcg ctttattgaa gcgatggacg acgatttcaa caccccggaa
    17941 gcctattccg tgctgtttga tatggcgcgt gaagtgaacc gtctgaaagc agaagatatg
    18001 gcagcggcga atgcaatggc atctcacctg cgtaaacttt ccgctgtatt gggcctgctg
    18061 gagcaagaac cggaagcgtt cctgcaaagc ggcgcgcagg cagatgacag cgaagtggct
    18121 gagattgaag cgttaattca acagcgtctg gatgcccgta aagcgaaaga ctgggcggcg
    18181 gcagatgcgg cgcgtgaccg tcttaacgag atggggatcg tgctggaaga tggcccgcaa
    18241 gggaccacct ggcgtcgtaa gtaattgcgc tattgccgga tgcgagtttt cgcatccggt
    18301 tatcgtctgc gccaccacaa cattcccatc agtagcatcc ccggcaacca cacccacatc
    18361 aattcagaaa taatcacctg atgcccgtac gctgtggtgt aacgagacaa tgcaaacggc
    18421 gcgactttaa tcacctgcca gggagcgaaa aagcgttcat ctgaccacgg ccacagccag
    18481 ccaacccctt taccgccagt ggttaccgaa tccagcaagc tgtgcgatag caacgagacg
    18541 gttaaaaaca gccagcagcg aatcagccca gccctgaacc atcgacgtcc aatcagcaca
    18601 cataacagcg ggacaacaaa cgcaaacacc agcgaatggg taaacccgcg atgaccaaaa
    18661 acattgccgt aagcaacgcc aaatttaaac gacaagacgt cggcatcggg cagcatcgcc
    18721 aggatgattc cggcaaataa cagacgcgga gggatgactt tcgaacccaa ccctaaacca
    18781 atgcataggg gaacggcggc gtgcgtaata acggttggca tgatggtcgc ttcggcaaaa
    18841 tgtcgatgct atcagcatgg atgaacgggg cgtagagggc aaaagtctga aaagagaacc
    18901 ggcctgttga tacaggccgg gaaagggatc aggcaacaac ctgtacgctg tgacctgcaa
    18961 aactcactgt ctgcccggcg acgattttgc agcgtttgcg cgtttcaacc gcgccgtcga
    19021 ctttcacctg gccttcggca atcgcgattt tcgcctgcgc gccgctttcg ctccagcctt
    19081 ccagtttcag caagtcgcac agctcaacgt gcggatgttt acctaaagaa aatgtcgcca
    19141 tgttactcac cctgtggatc atgatattca acgcacgcct gtagcgtgtt ttcaatcagc
    19201 gtggcaaccg tcatcgggcc aacgccgccc ggaacaggcg taatgtatga ggcgcgttta
    19261 gccgcgtctt caaacacgac gtcgcccaca actttgccat tttccagacg gttgatgccg
    19321 acatcaatca caattgcgcc ttctttgatc cagtcaccag gaataaagcc cggcttgccg
    19381 acggcaacga tcagtagatc ggcattttct acgtggtgac gcagattttt agtgaaacgg
    19441 tgggtaacgg tggtggtgca acctgccagt agcagttcca tgctcatcgg gcggccaacg
    19501 atattcgatg cgccaatcac cacggcgttg aggccgaagg tatcaatgtt gtaacgctca
    19561 agcagcgtga caataccgcg cggggtgcag ggacgcagac gcggcgcacg ctggcacaga
    19621 cgaccgacgt tataaggatg gaaaccgtct acgtctttgt ccgggtgtat acgttccagt
    19681 actttgacgt tatcaatacc cgccggtaac ggcagttgaa ccagaatgcc atcgatggtg
    19741 ttgtcggcat tcagcgcatc gataagctcc aacagctccg cttcgctggt ggtttccggg
    19801 aggtcataag agcgggagac gaacccgact tcttcacaag ccttgcgttt gcttgcgaca
    19861 taaatttgcg atgcagggtt actacccacc agcacaacgg ccagtcctgg tgcccgcagt
    19921 ccggctgcaa tacgcgcctg aactttttga gcaacttcag agcgcacctg ctgcgcaatc
    19981 gttttaccgt caataatctt tgctgccatc agagagagga ttccatctgt tacgtaggtc
    20041 gaaggggatg cgcctatttt gtcagaagcg gggcgcgctg tcaggtttcg tttcagattt
    20101 atcgcgtgaa gcgcctcttg cgaaggttag gcgcaccgtc gctgaggtcg ctgagattga
    20161 aagcttcatt tttcgtccat gatggcgttg taaatctgga actgacttat ttccttgtca
    20221 aaggattaag ataatttaag aaatacctga caata
//
LOCUS       DAZGCQ010000089        20091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000089 DAZGCQ010000000
VERSION     DAZGCQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.089"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHK07_003633"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHK07_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8103917.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            753..977
                     /locus_tag="ACHK07_003634"
     CDS             753..977
                     /locus_tag="ACHK07_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103918.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHK07_003635"
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHK07_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8103919.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHK07_003636"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHK07_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8103920.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHK07_003637"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHK07_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8103921.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTMGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHK07_003638"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHK07_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8103922.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALIRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHK07_003639"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHK07_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO8103923.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHK07_003640"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHK07_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8103924.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..8983
                     /gene="tssK"
                     /locus_tag="ACHK07_003641"
     CDS             7652..8983
                     /gene="tssK"
                     /locus_tag="ACHK07_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8103925.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8988..9749
                     /gene="icmH"
                     /locus_tag="ACHK07_003642"
     CDS             8988..9749
                     /gene="icmH"
                     /locus_tag="ACHK07_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO8103926.1"
                     /translation="MDEGSLSLPPFTGYDEKGQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            9758..12532
                     /gene="tssH"
                     /locus_tag="ACHK07_003643"
                     /gene_synonym="clpV"
     CDS             9758..12532
                     /gene="tssH"
                     /locus_tag="ACHK07_003643"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8103927.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIDMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYILPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSVVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEVAWHQQQTLVREIIALRQQLLGMAEDT
                     DAVVDTSSESESESESESESESEQDNTAAIPADDAGSVQPEETAETVSPVQRLAQLTA
                     ELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIKGQ
                     DLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTT
                     INMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNL
                     FYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPEIMQEALYPVLADFF
                     KPALLARMEVVPYLPLSKETLATIIAGKLTRLDNVLRSRFGAEVIIEPEVTDEIMSRV
                     TRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDAQDD
                     QSVTEDEPVL"
     gene            12529..13272
                     /gene="vasI"
                     /locus_tag="ACHK07_003644"
     CDS             12529..13272
                     /gene="vasI"
                     /locus_tag="ACHK07_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO8103928.1"
                     /translation="MSRMRVFRQTGWFIAGLMAGLPASAAPAETSSMAGVAVAVATAT
                     PLDAAATLQAMQSCRRESAALERLDCYDRLLEPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13277..14692
                     /gene="tssA"
                     /locus_tag="ACHK07_003645"
     CDS             13277..14692
                     /gene="tssA"
                     /locus_tag="ACHK07_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8103929.1"
                     /translation="MAMDLRDPNVWISHLLENLPDEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSPQTTATSESGVTQPSTPTPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMTLWQQVEKSVLLAPYWLDGHCISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLATQPGNQAAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            14801..18235
                     /gene="tssM"
                     /locus_tag="ACHK07_003646"
     CDS             14801..18235
                     /gene="tssM"
                     /locus_tag="ACHK07_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO8103930.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVQVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPSVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18246..19656
                     /locus_tag="ACHK07_003647"
                     /pseudo
     CDS             18246..19656
                     /locus_tag="ACHK07_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19622..>20091
                     /locus_tag="ACHK07_003648"
     CDS             19622..>20091
                     /locus_tag="ACHK07_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8103931.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDR"
BASE COUNT         4723 a         5497 c         5542 g         4329 t
ORIGIN      
        1 tgcacaggtt catacaatct atttaacgct tgcagaatac ccctctttac gatcgataaa
       61 aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga gtggtgattt
      121 tattttcacc gccatcaagg cggcatttcc gatatttttt gtggtacgaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgcct cgcgaacgta ttaatatatc gtatgtccca aaaacagatg gccagacggc
      301 tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac
      361 atcctcactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat
      421 ggctgaagca gccattggcc tcaattttac cgtccccgcc acgctgaagg gaagcacaac
      481 ggacgatgag ctgaatgtgg cgctgaatat caagtcactg gatgattttt caccagatag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaagag tcccgcgagc agttgctcaa agaaatcggc caggtgagca ataaataacc
      721 accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat atctccggga
      781 cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt
      841 ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc
      961 agcgcgtact tcactgacgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca
     1081 ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg
     1141 ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat
     1441 tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg gcggtgaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc
     1801 tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg
     1921 tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttt cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgcg gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgccgacc tgactatggg tgaacagcat attcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta
     3841 ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc
     3961 cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagatgga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga
     4201 atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc
     4261 gtcacaaatt cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc
     4321 ctttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca
     4381 ctggtccctg ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgctaact ttgatcgcca cgcaatccac catccgcaga cggcgcggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt
     4561 tacgggtatt cccgtccggg ggctggcctc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc gctgatacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg ctttgatttg
     5401 tcagaagtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca gctcagtatt
     5581 accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg tcagcagagc
     6001 attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct acgcccgcag
     6061 acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct gatacagttg
     6121 cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga
     6181 gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat
     6241 atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg aatgactcac
     6301 cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact ccaggcactt
     6361 cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc tggcgatcat aatgatgaca tctctgcggc agaaatggcg
     6541 caacgccacc ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccgtgagc
     6601 aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtgatgttt
     6781 gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcggcgattg tggagtcgct tcgtgtcctg
     6901 ctggacgcct tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gcgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggaacaa ccttcggaaa
     7261 taaccgtcac cctgctgact gaaccggaca ccaataccaa tgcggagggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacatgactg acgactcaaa attgcaggca gccgactatg
     7381 accagatagc cagtacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat cggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gcccacacca tttccagcaa cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcacact
     7861 gcctgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaaaatttt ccggacgaaa gaagccgcaa tatttacctc gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ctgcattcag aagaaggcga cgcgcgaaca ctgacgctgg gacaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagtgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg cattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt gcttaaccgt aaccagaccc ggtttaccca
     8401 tcgcgcccgc cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgac ctgaccaaaa catttaaaac actgttaccg gaagtcaaac gggcgctgca
     8581 taccgtactg tcgccaagag cggtcaatct gccgctgcat ctgcgtgacg gcatctggca
     8641 ggccgatgtt catgactcgg aactgctaca gtctgccacc tttgtactgg ccgtggcggc
     8701 aaatatgcct gtcgatcaga tccagcgtca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatccccggc attccattgc gtgccctgat
     8821 ggtggctccc cgtcagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag
     8881 cggacaggcc tggacagaaa tggctgccgc cggggccgtt gcactgcatg tatccggcag
     8941 tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt
     9001 cactgtctct gccaccattt accggatacg acgaaaaagg ccagcgcgac taccatctcg
     9061 cgcttcgtgg caacagcctg aatccgatga tcgacgcagc cacaccgcta ctcggtatgg
     9121 taatgcgcct ttccaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg
     9181 tgacggatgt tcaggccgtg gagcaactgc ttcaggaaca gggctacgaa ccgggcgtga
     9241 ttatctcgtt tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct
     9301 ggtcgaacaa gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct
     9361 ggggtggcga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc
     9421 aggatctgct ggaatttctc tggctctgtt tttctctggg atttcgcggg cgctacaaag
     9481 tggcagcaca ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc
     9541 ataaactgcg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg
     9601 ggcgttatca gctcatcagt cgcctgacca ttaaacatat tttttgtggt ggcgttgtcg
     9661 tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc
     9721 tgtaccagct aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac
     9781 gctggtaaaa cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctcagaatg
     9841 tatgagccag caggcagcgg aaattaccgt cagccatgtg cttattcaga tgctcgccat
     9901 gccacgcagt gacctgcggg ttatcactcg gcagggcgat attgacatgg aagagttgcg
     9961 ccaggcgctg acggtggaga actacacaac cgcccgttct gcggacagct acccggcgtt
    10021 ttccccgatg ctggttgagt ggctgaaaga gggctggctg ctggcgtcgg ctgagatgca
    10081 gcacagcgaa ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta
    10141 tatactgcct gctgccgccc ggctgttgac cggtattaac cgtgaccgtc tgcaacagga
    10201 ctttgtgcag tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg
    10261 cgcaggcaca ctgacggacg cctctgacac cctgcttgcc cgctacgcca aaaacatgac
    10321 cgaagatgcc cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct
    10381 gatgatcgac attctctgcc gccgccgtaa aaacaacccg gtggtggtgg gcgaagcggg
    10441 cgtgggcaaa agcgcgctga ttgaaggtct ggcgctgcgc atcgtggcag gtcaggtgcc
    10501 ggacaagctg aaaaacaccg atatcatgac ccttgacctg ggcgcattgc aggccggggc
    10561 atcggtgaag ggcgaattcg aaaaacgttt caaggggctg atggcggagg tcatttcctc
    10621 tccggtgccg gtcattctgt ttatcgacga agcgcatacc ctgattggcg cgggcaacca
    10681 gcagggcggg ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa
    10741 aaccatcgcc gccaccacct ggagcgagta caaaaaatac ttcgagaaag acgccgccct
    10801 gtcgcgccgc ttccagttgg tgaaggtcag cgaacccaac gccgccgaag ccaccattat
    10861 tctgcgtggt ctgtcggtag tgtatgaaca gtctcacggc gtgctgattg atgatgacgc
    10921 cttgcaggcc gcagcaacgc tgagcgagcg ttatctctcc gggcgtcagt tgccggacaa
    10981 agcgattgat gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt catcgccacc
    11041 aaagcaaatc tcggcgctga ccaccctgag ccaccagcag gaggcggaaa ttcgccagct
    11101 tgagcgcgag cttcgcatcg ggctgcgtac cgacacctca cggatgaccg aggtgctggt
    11161 gcagtatgat gaaacgctga cggcgctgga tgaactggaa gtcgcctggc atcagcagca
    11221 gacgctggta cgggaaatta tcgcgcttcg ccagcagtta ctgggtatgg cagaggacac
    11281 agatgccgta gtggatacgt cgtcagagtc agagtcagag tcagagtcag agtcagagtc
    11341 agagtcagaa caggataata ccgctgccat accggctgat gatgctggca gcgtacagcc
    11401 ggaagaaacc gctgaaacag tttccccggt acagcgactg gcacagctta ctgccgaact
    11461 ggacgccctg cataacgacc ggttgctggt ttccccgcac gtcgataaaa aacagattgc
    11521 ggcggtgatt gccgaatgga ccggcgtacc gcttaaccgc ctgtcgcaga atgaaatgtc
    11581 ggtcatcacc gacctgccag tatggctggg cgacaccatc aaaggccagg atctggcgat
    11641 tgccagcctg cacaaacacc tgctgactgc acgcgccgac ctgcgtcgtc cgggacgtcc
    11701 gcttggcgcg ttcctgctgg ctggccccag cggtgtgggt aaaaccgaaa ccgtcctgca
    11761 actggcggag ctgctctatg gcggtcgcca gtacctgacc accatcaata tgtccgagtt
    11821 tcaggagaaa cacactgtct cgcggctgat tggttcccct cctggctatg tcggctatgg
    11881 cgaaggcggc gtactgaccg aagccatccg ccagaaaccg tactcggtgg tactgctcga
    11941 tgaagtggaa aaagcgcacc ctgatgtgct caacctgttc taccaggcgt tcgacaaggg
    12001 cgagatggca gacggcgaag gccgcctgat tgactgtaag aatatcgtct tcttcctcac
    12061 gtccaacctc ggctaccagg tgattgtcga acacgcggat gaccccgaga tcatgcagga
    12121 agccctctat ccggtgctgg cggacttctt taaacctgcc ctgctggcgc gaatggaagt
    12181 ggtgccgtac ctgccgctgt cgaaagagac gctcgccacc atcattgccg ggaaactcac
    12241 ccgtctggat aacgtgctgc gcagccgctt tggcgcagaa gtgattatag agccggaagt
    12301 gacggacgaa atcatgagcc gtgtcacccg cgcggaaaac ggcgcaagga tgctcgaatc
    12361 ggtcatcgat ggcgacatgc tgccgccgct ctcgctgctg ctgttgcaga aaatggcggc
    12421 caatacggcg attgcccgta tccgcctgtc ggcggcagac ggcgcgttca cggcagatgt
    12481 ggaagatgct caggacgacc aatccgtcac agaggatgaa ccggttttat gagcaggatg
    12541 cgcgttttcc ggcaaacagg atggtttatc gcgggcctga tggcgggctt acccgccagt
    12601 gccgcgcccg cagaaacatc gtcaatggcc ggtgtggcgg tagccgttgc cacagcgacc
    12661 ccgctggatg cggcagccac gttacaggcc atgcagtcct gtcggcggga gtctgcggcg
    12721 ctggagcggc ttgactgcta tgaccgcctg ctggaaccgc tgtccccgtc agggtttgac
    12781 ggggcgctgg tcaaagctgg ttttgtgggc gaagcctgga cccgcgccac agagcaggaa
    12841 aaacgccgcc agggcaacac cacagagctg ctggtgacgc aggtgccggg ggaacgcccg
    12901 acggtggtga taaccacccc ggctatcggc catgttccgc cacgcccggt gctgatgttc
    12961 agttgcgtgg acaatattac ccggatgcag gtggcgctga tgcaccctct ggatgtccat
    13021 gatatcgccg tcaccctgaa tgccgacagc cgggcattac gcagccactg gtttgtgcgc
    13081 gagaacggca ccctgctgga gtccagccgg gggttatccg gcattgatga aattaagcaa
    13141 ctgttcggcg caaaaacgct gacggtggat actggcgcag acaacgccgc cggaaagctg
    13201 acctttaaca ttgacgggct agcaagggcg attgctcccc tgcgcgatgc ctgccactgg
    13261 gcgggagaat aaaaatatgg caatggactt acgcgatccg aatgtctgga tatcgcacct
    13321 gctggaaaac ctgccggatg agaaactggc atcggcgttg aaagacgaca acccggactg
    13381 ggagtatatc gacggcgaaa tcgtcaaact ggggtcactg gcccacagcc agctggatat
    13441 cccggaactc cagcgccggg ggctggtcat tctggcttcg gagagtaagg attttcgtct
    13501 gctggcgcat ctgctgagaa ccctgcaaca cgccggtgat cctcatctcg cattgcgcct
    13561 gctggcgctg tatgtggagc attactggtc ggtggctgcg ccgcagaata tggcacataa
    13621 aaaacgcttt gccagccagg tcattaaacg ctttgaaaca ggtattgaag gcttttcaca
    13681 gaacgccgcc acagcgcagc gcgacgcgct gtcaggggaa ctggcgaaac tggcgcagtg
    13741 ctggcagtca cataacgccc cggaactggc actggctacc gatgatcttt ttgccctgta
    13801 ccagcgtgcg tttaatcgtg cggcaccggc tcctgccccc actccggcgg cctccgatag
    13861 ttcaccacag accaccgcca cgtctgaaag cggcgtgacg caacccagta ctccgactcc
    13921 ccaaatcgcc atcgacagtc acgacgacaa agcctggcgc gacacgctgt taaaagtggc
    13981 ggctatttta tgtgaacgcc agccggactc gccgcagggc tatcgcctgc gccgccatgc
    14041 cctgtggcaa tccatcacca gtacacccca ggcggaaagc gatggacgta ccccattggc
    14101 tgcggtctct gccgatatgg tggcggatta ccagtcccgg cttgccagcg cggatatgac
    14161 gctgtggcag caggtggaaa aaagcgtact gctggcaccc tactggctgg acggtcactg
    14221 catttctgca cagacggcgc accgtcttgg ctataccgct gtggccgatg ccatccgcga
    14281 tgaagtcgtt tcctttcttg gccgtctgcc cgcgctcgct gatcttctgt ttaacgacca
    14341 cacaccgttt gtttctgaaa aaacgaagca atggcttgca actcagccag gtaaccaggc
    14401 cgcgccagtc atacaggtga gtaatgaaga tattcaggct gcgcggttgt gttttgatga
    14461 acaggggctg gaagccgcct tacggtatct ggatacgttg cccgccggtg aaccccgcga
    14521 ccagtttcac cggcagtatt ttggcgcgca gcttatggaa gaggcggggc tggtacagct
    14581 tgcgcagcag cagtaccgga tgttgttcag gatgggattg cagatgatgg tggcagactg
    14641 ggagccgtcc ctgcttgagc agctagaaca aaaattcacg gcagaacaat aagcgataac
    14701 aggagttttt gtgttcagat taccaacacc ccgactactc agcggactca aatcagccct
    14761 acgcccggcg atgccccggt ttaaagtctc cgctttctgg ctgctgatac tggcgtggat
    14821 ttttctgctg gtatggatct ggtggaaagg cccgacgtgg acgctgtatg aagaacagtg
    14881 gctcaaacca ctggcgaacc gctggctggc aacggcggca tgggggatta tcgccctggt
    14941 gtggcttacc gtccaggtga tgaagcgcct gcaacaactt gaaaaaatgc aaaagcagca
    15001 gcgcgaggag gccattgatc cgctcagtgt ggagctgaac gcccagcagc gttaccttga
    15061 ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt ggcagttgcc
    15121 gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgc gcgaggggtt
    15181 tccgtcagac attatttatg ccccggaggg cgcgcgcggc acagaacaac gcctgtacct
    15241 tacgccccat gtcggtaaac aggcggttat ctttgatatc gacggcacac tctgcgctcc
    15301 cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct ggctgaaaga
    15361 aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac ccgatctgct
    15421 taccgcagac aaacgccgcc gcgagcatct gttacagacg ctgcgcagcc gcttgcagga
    15481 tatacgccag catcttcact gccagttacc ggtttacgtg gtactcaccc ggcttgattt
    15541 attgcagggt tttgccgccc tgttccagtc actgaacaga caggatcgcg atgcgattct
    15601 gggcgtcacg ttcacccgcc gcgcccatga aaatgatgac tggcgaacag agttgaatgc
    15661 tttctggcag acatgggtgg atcgaatgaa cctagcgttg ccggatctga tggtcgctca
    15721 gactcacacc cgcgcgtctt tatttagttt ttcccgccag atgcagggaa gccgtgaacc
    15781 gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat atgaacgtga tgctgcgtgg
    15841 tgtctatctc acctcttcgc ttcagcgtgg acagatggat gatatattca cccagtctgc
    15901 cgcccgccag taccggctgg gcaataaccc actggcttcc tggcctctgg tggacaccgc
    15961 gccttatttc acccgcagct tgttcccgca ggcattactc gcagagccta atctggcaac
    16021 ggagagccgc gcctggctga tgcgttcccg tcgccgcctg acggttttct ctaccacagg
    16081 aggcgtggcg gcactgctgc tcatcaccgg ctggcatcac tattacaacg gtaactatca
    16141 gtccggcatc accgtgctta agcaggccaa agcctttatg gacgtgccgc ctccgcaggg
    16201 ggaagatgac tacggcaacc tgcaactgcc gctgctgaac ccggtacgtg atgccacact
    16261 ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt accaggggcg
    16321 acgtatcggg ccatatgtgg aacagaccta tctgcaactg ctggagcaac gttacctacc
    16381 ctcgctgttt aacgggctgg ttaaagcgat gaatgccgcg ccgccggaga gtgaagaaaa
    16441 actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa agcggacgta acaatgaggt
    16501 ggtgaagcag tatatggcaa aacgctggag cgaaaagttc cacggccagc gcgatatcca
    16561 ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc acgcagagcg
    16621 tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc ccgtggtatc
    16681 agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga gcctgaaaac
    16741 gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag ggccaacctt
    16801 tgatcaggta tttacatctg ccgatgacaa caaactggtt gttccacagt ttcttacccg
    16861 ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac tgacggcgat
    16921 ggattcctgg gtacttaacc ttacccgcag cgtgaaatac agtgacgccg accgcgcgga
    16981 aatccagcgc cagctcaccg agcagtatat cagcgactac accgccacct ggcgggccgg
    17041 gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg gggcgctgga
    17101 gcaggttatc agcggcgacc agcctttgca gcgggcgctg accgtgctgc gtgacaacac
    17161 acagccaagc gtcttttctg aaaaactctc tgccaaagaa cgggatgagg ccctggcaga
    17221 gccggactac cagttactca cccgtctcgg gcatgagttc gccccggaaa acagtaccct
    17281 ggcagtacag aaagacaaag aaagcacgat gcaggcagta tatcagcaac tcaccgagtt
    17341 gcaccgctat ctgctggcga tccagaacgc gcctgtacca gggaaatcgg cgctgaaagc
    17401 cgtgcagtta cggcttgatc agaacagcag cgatccgata ttcgccaccc gccagatggc
    17461 aaaaacgctg cctgctccgc tcaaccgctg ggttggcagg ctggctgacc aggcctggca
    17521 tgtggtgatg gtggaggccg ttcactatat ggaagtggac tggcgcgaca gcgtggtgaa
    17581 accgtttaac gagcaactgg caaataacta tccgtttaat ccgcgttctg cacaggatgc
    17641 ctcactggat gccttcgaac gcttctttaa accggatggc atactggata ccttctacca
    17701 gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg ataacaacgt
    17761 cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc gtgacatctt
    17821 cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat cgctttcagg
    17881 caataaacgc cgcagtgtac tgaaccttga cggtcagtta gtcgattaca gccagggccg
    17941 taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg aaagtaagct
    18001 gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg ggccgtgggc
    18061 gcagttccgc ctgttcgggg ccggacaact gaccggagta caggatggca actttaccgt
    18121 gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca cggaagataa
    18181 cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt actgaggagg
    18241 cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac ccgcgcgggc
    18301 tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc cagccacccg tcacagcctg
    18361 agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc aacggtgtgg
    18421 atttacacac cgccacctac tatacgcttg cccggacacg gatacaggga ctggcgggat
    18481 tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat aagttctggc
    18541 cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga accggcaata
    18601 ttctgcgtca gcaaatctcc tttgcggaat ctgatctgcc gctgatctac cgcacagagc
    18661 gggcattgca gcttatctgc gacaagcttc agcaggtaga gctgaagcgc gttccgcgcg
    18721 tggagaatct gctctatttt atgcagaaca cgcgtaaacg gcttgaaccc cagctgaaga
    18781 gtaacactga gaacgccgca cagaccacgg tcagaacgct gatttatgcc ccggaaacac
    18841 agccatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctgcct gagatgaaag
    18901 tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag caaggcagta
    18961 cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca gcattgtggt
    19021 ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc gctcagggcg
    19081 cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg ctgcaacaac
    19141 ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg cgtgttgccg
    19201 acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg aatgaggcac
    19261 tcaaaacccg cgcacagaat agcccgcagt tgcgtggctg gttgcagacc cgccaggact
    19321 tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactaacc ctttcctata
    19381 tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaacaccc gttgagtctc
    19441 tgttgacgca gtaccaggat gcccgtgcgc agaagcagaa tacagatgcg ctggaaaaac
    19501 aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaat aataatacga
    19561 taccgacgat aaaaaaaggc attgaatttc aataacattc atgaatacaa aggagtttta
    19621 aatggcgaat ttaatttatt taacactaaa tggtgataat caagggctca tttcaagtgg
    19681 ttgttcatca caaccatcca ttggaaataa agctcaaaca gcacacattg atcaaattat
    19741 gatttatgaa ttcatgcatg gattaaatcg agatcaaaac gtcaaccatc atcatttgac
    19801 aataaaaaaa cctatagata aagcatcacc cttattaggc aaagcaattt gcgacaatga
    19861 attattgaca tgtgattttt ccttttatcg tacaaataaa tttggaatta atgaactgta
    19921 ctataaaatc aaattaactg gagcaaagat aagcgacatt catgtctcta tatcacatat
    19981 tgttgttgat aacagcgttc aacccgaaga gtcagtttct ttcagttatg agtcaattat
    20041 atgggagcat tgttccgctg gcactagtgc atacagttta tgggaagatc g
//
LOCUS       DAZGCQ010000090        19926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000090 DAZGCQ010000000
VERSION     DAZGCQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.090"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHK07_003649"
     CDS             complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHK07_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO8103932.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            400..474
                     /gene="yljB"
                     /locus_tag="ACHK07_003650"
     CDS             400..474
                     /gene="yljB"
                     /locus_tag="ACHK07_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO8103933.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            573..893
                     /gene="clpS"
                     /locus_tag="ACHK07_003651"
     CDS             573..893
                     /gene="clpS"
                     /locus_tag="ACHK07_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO8103934.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            924..3200
                     /gene="clpA"
                     /locus_tag="ACHK07_003652"
     CDS             924..3200
                     /gene="clpA"
                     /locus_tag="ACHK07_003652"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO8103935.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(3605..3692)
                     /locus_tag="ACHK07_003653"
     tRNA            complement(3605..3692)
                     /locus_tag="ACHK07_003653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Ser,seq:gga)
     gene            complement(3946..4164)
                     /gene="infA"
                     /locus_tag="ACHK07_003654"
     CDS             complement(3946..4164)
                     /gene="infA"
                     /locus_tag="ACHK07_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO8103936.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4449..5153)
                     /gene="aat"
                     /locus_tag="ACHK07_003655"
     CDS             complement(4449..5153)
                     /gene="aat"
                     /locus_tag="ACHK07_003655"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO8103937.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5195..6916)
                     /gene="cydC"
                     /locus_tag="ACHK07_003656"
     CDS             complement(5195..6916)
                     /gene="cydC"
                     /locus_tag="ACHK07_003656"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO8103938.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNTGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6917..8683)
                     /gene="cydD"
                     /locus_tag="ACHK07_003657"
     CDS             complement(6917..8683)
                     /gene="cydD"
                     /locus_tag="ACHK07_003657"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO8103939.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8806..9771)
                     /gene="trxB"
                     /locus_tag="ACHK07_003658"
     CDS             complement(8806..9771)
                     /gene="trxB"
                     /locus_tag="ACHK07_003658"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO8103940.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10316..10810
                     /gene="lrp"
                     /locus_tag="ACHK07_003659"
     CDS             10316..10810
                     /gene="lrp"
                     /locus_tag="ACHK07_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO8103941.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10945..15051
                     /gene="ftsK"
                     /locus_tag="ACHK07_003660"
     CDS             10945..15051
                     /gene="ftsK"
                     /locus_tag="ACHK07_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO8103942.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15206..15817
                     /gene="lolA"
                     /locus_tag="ACHK07_003661"
     CDS             15206..15817
                     /gene="lolA"
                     /locus_tag="ACHK07_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO8103943.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15828..17171
                     /gene="rarA"
                     /locus_tag="ACHK07_003662"
                     /gene_synonym="mgsA"
     CDS             15828..17171
                     /gene="rarA"
                     /locus_tag="ACHK07_003662"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO8103944.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIILPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17262..18554
                     /gene="serS"
                     /locus_tag="ACHK07_003663"
     CDS             17262..18554
                     /gene="serS"
                     /locus_tag="ACHK07_003663"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO8103945.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(18795..18935)
                     /locus_tag="ACHK07_003664"
     CDS             complement(18795..18935)
                     /locus_tag="ACHK07_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8103946.1"
                     /translation="MSQKSLMAITFCVTAILIIWMLHGSLCEIRMNIWGAEFAAFLQC
                     KQ"
     gene            complement(19264..19404)
                     /locus_tag="ACHK07_003665"
     CDS             complement(19264..19404)
                     /locus_tag="ACHK07_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8103947.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(19539..19841)
                     /locus_tag="ACHK07_003666"
     CDS             complement(19539..19841)
                     /locus_tag="ACHK07_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8103948.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
BASE COUNT         5028 a         5003 c         5285 g         4610 t
ORIGIN      
        1 ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa
       61 taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag
      121 cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc
      181 cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac
      241 ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg
      301 gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg
      361 gcgagccgtc aagcatttga caggggacaa ggggcaggta tgaatcaaaa atttgaagca
      421 gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta
      481 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
      541 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
      601 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
      661 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
      721 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
      781 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
      841 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
      901 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
      961 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     1021 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     1081 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     1141 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     1201 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     1261 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     1321 atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     1381 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     1441 cgacgaacct gaatcagctt gcgcgtgtgg gcggaatcga cccactgatt ggtcgtgaga
     1501 aggagcttga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     1561 tgggggaatc tggtgttggt aaaactgcga ttgcggaagg tcttgcctgg cgaattgttc
     1621 agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     1681 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     1741 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     1801 cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg ttgctctcca
     1861 gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     1921 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag
     1981 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     2041 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc
     2101 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     2161 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     2221 ttgcacgcat tccagagaag agtgtttctc agagtgatcg tgataccctg aaaaacctcg
     2281 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     2341 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     2401 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     2461 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     2521 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     2581 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccagacg
     2641 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     2701 aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta cgggaaactg
     2761 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     2821 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg ttcgatcatc
     2881 tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     2941 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     3001 aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag gacaacctga
     3061 aaaaaccgct tgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     3121 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     3181 aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg taatctcgtc
     3241 aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     3301 aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg tgctgcagga
     3361 gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc
     3421 cgtacaggca gcttagaaaa acctaccgtc ttggctagcg gttgcagcga acgttcactg
     3481 ccgtacaggc agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg
     3541 ccgtacaggc agataaaatg cgaaaaaaaa agctcgcact ttcgtacgag ctcttcttta
     3601 aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca cactttccag
     3661 gcgtgctcct tcagccactc ggacacctca ccaaattgtt ttgttgcctg acctcatggg
     3721 gggcaacggg gcgctactat agggagttgg agtaaaacgg tcaagaagaa ttttaatgat
     3781 aattattgtt tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta
     3841 cggacttata cttccaaagt acttcataca tatcacaaaa taaaaaggcc ggttaaaccg
     3901 accttttact cgttctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa
     3961 gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt
     4021 caggatgcgg atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc
     4081 gttttctaac tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat
     4141 ttcaatattg tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc
     4201 ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa
     4261 caggttcggc acattacccc gaaaacacac ggctaagccg caccaaaagc gcaacgtata
     4321 agggagcggt gagataaacg atgggcgtta cctgacgcga aaaattcctt atcggcagcg
     4381 gggtaatgag cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc
     4441 gaaaacattc attcttgtgg tgaaaacaag catcgtggta cccagaaatt attcggcaat
     4501 cgtccgaggc gcatttgatt gagataattc agataatccc gacggggaat ttcgcaggca
     4561 ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga taagcttacc gccatgaccg
     4621 ataaattcct cacagaatac cagaagcgcc gttttagacg cattttccat ccggctgaac
     4681 atggactcgc cacaaaatag cgttccctgg gccacgccgt acataccgcc gacaagctca
     4741 tcttcacgcc agacttcaat ggagtgggca tgaccgagtt cgtgaaggcg atggtaggct
     4801 tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcaatg
     4861 acctgaccaa aagcgtaatt catcgtgaca cgatagggcg agcgtttatg aaatcgcttc
     4921 atactacggc tgatatgcag tgattctggc catagcaccg cgcggggatc gggcgaccac
     4981 cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta agccattaac
     5041 aggcgcgcag ggctaagatc gcccccaagt gccagcaggc cgttaggctc acgtaatgcg
     5101 ccttccgggg aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca
     5161 aaactccacg caagtcggat cgttcaataa tagcttacaa accctgcttg aactggtaat
     5221 aacgcccctg tctggcaagc agttctgcgt gagtaccttg ctcaataatt tgcccgttgt
     5281 ccatcactat tatttgttgg aaacgagaga gcccgcgaag tcgatgggtg accattaaca
     5341 ccgttttctc acgcatcatt tctgcaagca attcaaggat ctggctttcg gttgtggcat
     5401 ctaagccttc ggtaggttca tccagcaaca ccagtggcgc atcatgtaac agcgcacggg
     5461 cgatagccag acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac
     5521 tgttgagacc tgcatcctcg agcagcttct ccagaccaac gcgacgcaag atcaccgcca
     5581 gagcctcatc actactgcca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa
     5641 acagatgcac tcgctgagga acaacgctga tggtctgtcg tagagccgct tcattcaggc
     5701 tggctatggg gctatcgtta agcaaaatct cgccctgttg cgggtcccat gcgcgggtca
     5761 gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc gagaatcgca atatgttctc
     5821 cggtgtttac ctgaagagaa atccctttaa gtgcctgttg ggattgctcc ggataagtga
     5881 actgaacatc ccgtaacgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga
     5941 aggtgacctc cggtttttga tccgttaagt cagtgatccg aacggcagag gcaatgactt
     6001 gccccagatg ctgaaatgca cctgttactg gtgccagtgc ttcaaacgcg gctaacgcgc
     6061 agaagacaaa cagggcaatt aacgcgccag gttgagcatt gccgccaacg ccgccagacg
     6121 ccatccacag catcaggatc accgctaacg cgccaatgag cagcattatc gcttgcgaca
     6181 atgcggtcag ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta
     6241 gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc
     6301 aggccgtcag ttgttggcga tactgtccgc gaagatgagt cagattttgc ccggtgcttt
     6361 ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt cagtaacata atgccgccca
     6421 gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat tgtcaccacc ataatcacca
     6481 caaaagcgcc caccagcggc gagataacgc gcaggtaaag atgatcgagc gtatcaacat
     6541 ccgccaccac gcgattgagc aattcgccct gacgatagcg cgccagtccg gcaggggaga
     6601 ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt
     6661 cgtgacttac cagacgttca aaataacgcc cggcagtacg ggtgattgct gcaccacgca
     6721 cgcccgcagc gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg
     6781 aggccgagag gaaccagccg gaaagtgtca acagaccgat actggcgagc agcgtcacaa
     6841 ttgccagcac aataccaaga cttaacatcc atttatgacg tttatacagt gccagatagg
     6901 gtagcaaagc gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc
     6961 caccagccac acttaattcc gcgtaacgtc cttgctcaat aatccggcca tcctgcataa
     7021 cccaaatgac atcccagtca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct
     7081 ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc
     7141 tggcagcggg ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg
     7201 ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa
     7261 cgccttgtgg caggagcggt agaaactcgc tgacccaggc gttatccagc gctgcttgta
     7321 attcttgttc gctggcatca ggtcgcgcca gtagtacgtt atcccgcaat gttgctgccg
     7381 gtaattgtgg gttttgccca acccaggaga gatgtttacg ccatgattct ggtgataaat
     7441 cgcgtaattc tatcccgttg attcgtaatg atccctgata tgagagaaaa ccagaaagcg
     7501 cgttcagcag tgagctttta cctgaaccgc tgcgaccaac caacactgca cgttggcctg
     7561 ctggcaaagt aaagttcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca
     7621 gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa ttccgcctca ccgcgctgcg
     7681 gatgggcgag cggggtttcc ataaacgttt tcagactatc agctgcacca acagcctggg
     7741 ctttagcatg ataaaacgta ccgagatcgc gtaatggctg gaaaaactct ggcgcaagga
     7801 tcagggccag aaaacccgca gccagcgtca cgccagtatc gtagtgacca aaatccagct
     7861 cgccgagata ggaaaaacca aagtagaccg ccaccagagc aattgacagc gaggtaaaaa
     7921 attcgagaat gccggaggat aaaaacgcca gtcgtagcac ttccattgtc cgttggcgga
     7981 aatcttccga agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca
     8041 atgtttccat gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa
     8101 agttacgtcg gttagcatcg gcagccccca tcccaaccag cgccataaac aacggaatta
     8161 acggtgcagt gcccagcaga atgagcgccg cagcccagtt agaggggaag atcgccacca
     8221 caatcagcaa cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat
     8281 catgcatatc gtcaatttgc tcgagtacca gcgtcgccca gctccccgca ggtttaccct
     8341 gaatccacgc tggccctgct tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc
     8401 ggatatgctg cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgctcgca
     8461 gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct
     8521 ccataatcat atgttgcaga attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc
     8581 cgcttacaaa gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct
     8641 gttttaacca gcgggttaac tctttttgac gagatttatt cattgcacgc ttagcaggtg
     8701 agttatcaga attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata
     8761 gtcgcaaggt gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc
     8821 taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg cagccagtac cggccgaagt
     8881 aatggcctgg cgataaatgt gatccatcac gtcgcctgcg gcaaagacgc caggaatgct
     8941 ggtctgggtg gcattaccat gaatacccga ctgtactttg atgtagccgt tttccagttc
     9001 cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg atagcaacaa acagaccggc
     9061 aacgtcgagt gactcgatgt tgtcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac
     9121 acccatttga tcgccggtca cttcttccag cgtacggttg gtgtgcagaa tgatgttgcc
     9181 gttctccact ttatccatca gacgcttaat aaggattttt tccgcgcgga aaccgtcacg
     9241 gcggtgaatc agatgcactt ccgaagcgat gttagacaga tacagcgcct cttcaaccgc
     9301 ggtattgccg ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt
     9361 tgcacaagca gaaactccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg
     9421 tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt
     9481 cagacggaac ggacggtttt gcagatccac cttgttgata tgatcaaaaa tgatctcagt
     9541 ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt
     9601 tggatcgcca ggccagtttt ccacttccgt ggtggtggtc agttggccgc ctttttccat
     9661 gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg tagacagcag cggtgtatcc
     9721 cgccgggcct gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc
     9781 atagttgttg gcagacaatg ggcaggattg tagggaattt acagacgtaa aaaaagagta
     9841 tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc aaattctccc
     9901 gtcattatca cctctgctac ttaaatttcc cgctttataa gccgattaaa tgatgaataa
     9961 acgcccctgt taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa
    10021 tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa acagtattct tatatgcgca
    10081 taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca
    10141 gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag
    10201 gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac
    10261 agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt
    10321 agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga
    10381 gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc
    10441 aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac
    10501 ggcgctgctg aacccccatt atctggatgc atcacttctg gtattcgttg agattactct
    10561 gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga
    10621 aattcaggag tgtcatttag tatccggtga tttcgactac ctgttgaaaa cacgcgtgcc
    10681 ggatatgtca gcctaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa
    10741 tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa
    10801 gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc
    10861 atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa
    10921 agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca
    10981 ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct tattgtcctg
    11041 tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg
    11101 caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggttggca
    11161 gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc
    11221 ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat acattgatta ttttgccgtt
    11281 tcgctacgca tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc
    11341 aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact
    11401 acgctacaac cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggca
    11461 gcgggcctga cgttgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc
    11521 tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc
    11581 gatgaagatg aatatgaaga tgacgaagag tatgaagatg aaaaccacgg caaacagcat
    11641 gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg gttggcggaa
    11701 aaattcatta atccgatggg gcggcaaaca gatgctgcgc tgttctccgg taagcggatg
    11761 gatgatgaag aagatattac ctacactgca cgcggtgtgg ctgccgaccc tgacgacgtc
    11821 ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaat
    11881 ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc gacacaaagc
    11941 tgggctgcgc cggttgaacc tgtgactcag acgccgcctg ttgcctctgt tgatgttcca
    12001 ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtt
    12061 attgctcctg caccggaagg ttacccacag cagtcacaat atgcgcagcc tgcagtgcaa
    12121 tataatgagc cgctgcaaca accagtacag ccgcagcagc cgtattatgc acctgcagct
    12181 gaacaacctg cgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcaaccg
    12241 tattacgcaa ctgcgccgga acagcctgcg caacagccgt attatgcccc tgcgccagaa
    12301 caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag
    12361 tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa
    12421 cagccgcaac ctgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca
    12481 aaacccgcgc gtccgccgct ttactacttt gaagaagtgg aagagaagcg agcccgtgaa
    12541 cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg
    12601 atcaaatctt cgctgaaagc accttctgtt gcagcagtac ctccagtaga agccgctgcc
    12661 gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca
    12721 accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa
    12781 gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa
    12841 ttggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagaaaa agcccgtgaa
    12901 gcccagcgca atcagtacga ttctggcgat cagtacaacg atgatgaaat cgatgcgatg
    12961 cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag
    13021 tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg
    13081 gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg
    13141 aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt
    13201 ccacacgaac cgttgtttac gccaattgtt gaacctgtac agcagccgca acaaccggtt
    13261 gcaccgcagc cgcagtatca gcagccgcaa cagccggttg caccgcagca gcagtatcag
    13321 cagccacaac agccggttgc gccacagcag caatatcagc agccgcaaca gccggttgca
    13381 ccgcagccgc agtatcaaca gccgcaacaa ccagttgcgc cgcagccgca gtatcaacag
    13441 ccgcaacaac cggttgcacc gcagccgcag tatcagcagc cacaacagcc agttgcgcca
    13501 cagccgcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg
    13561 cacaaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg
    13621 gagccggtag atacctttgc gcttgaacaa atggcgcgtc tggtggaagc gcgtctggct
    13681 gatttccgta ttaaagccga tgtcgtcaat tactctccgg ggccagttat cactcgcttt
    13741 gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt
    13801 gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat
    13861 gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga agttttggat
    13921 aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc
    13981 ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc
    14041 ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag
    14101 ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa
    14161 ggcattccgc acctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg
    14221 cgctggtgtg ttaacgagat ggagcgtcgg tataaactga tgtctgcgct gggtgtgcgt
    14281 aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca
    14341 gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa
    14401 ccatacattg tcgtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa
    14461 gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta
    14521 ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg
    14581 acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct
    14641 ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa ctccacgttg
    14701 ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac
    14761 tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cctccgacag cgaaagcgaa
    14821 ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg
    14881 cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacaacgtca gttccgcatt
    14941 ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa
    15001 caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat
    15061 tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt
    15121 tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg
    15181 gagtgacgta atttgaggaa taatgatgaa aaaaattgcc atcacctgtg cattactctc
    15241 aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga
    15301 taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc
    15361 ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat
    15421 gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc
    15481 gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat
    15541 gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga
    15601 tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt
    15661 gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag
    15721 ttatcaactg aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc
    15781 gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt
    15841 cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt
    15901 tagcacagta tatcggtcag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta
    15961 tcgaagccgg gcatttacat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa
    16021 ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca
    16081 cctctggtgt gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac cgcaatgcag
    16141 gtcgccgcac tattcttttt gttgacgaag ttcaccgttt caacaaaagc cagcaggatg
    16201 catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc actgaaaacc
    16261 cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtttatctg ttgaaatccc
    16321 tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa acccgtggct
    16381 atggtggtca ggatattatt ctgccagatg aaacacgacg cgccattgct gaactggtga
    16441 atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtcg
    16501 atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgcc ggtgaacgta
    16561 gcgcccgctt tgataacaaa ggcgatcgct tttacgatct gatttccgca ctgcataagt
    16621 cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt attaccgctg
    16681 gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa gacgtcggta
    16741 atgccgatcc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg
    16801 gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctggcc tgcgcgccaa
    16861 aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc gaacgcccgg
    16921 attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct
    16981 acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt
    17041 acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag
    17101 gcaagattgg cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac
    17161 gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg
    17221 tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc
    17281 tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg
    17341 tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc
    17401 tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag
    17461 atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag
    17521 ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc
    17581 ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc agccgctggg
    17641 gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt gacgctgggt gaaatgcact
    17701 ctggcctcga ctttgcagct gcagttaagc tgactggttc ccgctttgtg gtaatgaaag
    17761 ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac
    17821 agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta
    17881 cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag
    17941 cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact aacctggtgc
    18001 gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac accccatgct
    18061 tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt
    18121 tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga ctcaatggcg gcgctggaag
    18181 agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac cgtaaaatca
    18241 tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga
    18301 tcccggcaca gaacacctac cgcgagatct cttcctgctc caacgtttgg gatttccagg
    18361 cacgtcgtat gcaggcacgt tgtcgcagca aatcggacaa gaaaacccgt ctggttcata
    18421 ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcggttatg gaaaactatc
    18481 agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg
    18541 aatatattgg ctaataccta atttttctga atctaaaaag cgcctgcggg cgctttttgc
    18601 atgaggttaa ttaactgaaa ataaaaggga tttattttca ggtgtccata taatgcccat
    18661 ctttgaaaca aaaaaagccc gcagttttta cgctgcgggc tttatttttc tttgtgactt
    18721 gcgtgtgagg gtgctacttt cggccttacc ctgacaaccg attgacgggg gattactccc
    18781 ccgtcgcggt ttccttactg cttacactgt aagaacgccg caaactccgc tccccagatg
    18841 ttcatccgta tttcacacag cgaaccatgt aacatccaga tgatgaggat tgccgtcacg
    18901 cagaacgtga tggccataag cgatttttgc gacatagcgc ttgctccttt gttggagagg
    18961 cgctaacctg tcacttgcta aggttagatg gttagggcct cgggttaacg tttaagttga
    19021 ctcggggcct ttccacatcc ggccttcagg taatccttcc agccgtcagc cgaaaggcac
    19081 ccacgtgtaa tctactgatt tccctttgtg cgcgcaatgc tcagtgttga aactgtttag
    19141 gggggcggca aaaaagcccg catattgcgg gcaaatacgt ctgtaatcca atggagaaag
    19201 gggtgccata tcgactgaac ccgacaaccg gatagcgggg gctttcaccc ccgctgcggt
    19261 ttcttactgt ttacactgca agaacgccgc aaattctgac cacatgatgt taagtctgaa
    19321 ctcacacggc gaacggtgta tcatccagat ggccaacaac atggttaagc ataaaatgct
    19381 cacgatgatc gttttttgcg gcatagcgct tgactccttt tacagggagg cgctaacctt
    19441 ccacttgtct agggtaggaa tggttagggc ctcgggttaa acaatatgtt tgactcgggg
    19501 cctttccaca tccggccttc aggttttcct tccagccgtc agccgaaagg cacccgcgcg
    19561 taatgtactg gttttaaagc cataccgcaa tcctccagtt gtagcgttaa ctgactgcgt
    19621 ccctgatggg atttgcgcat tgtctcccgt ggaatatctt tatcgtggaa gatcaactga
    19681 ggctggccaa taattctgtg cacactttcc attgttgaaa atgtggcgct gcactcaagg
    19741 ttattgcact gatagtattt gtagcggatg gtgccggtat cattggcggc tttactggta
    19801 cggatccggg cagatgcgcc gcagaatgga caatgaaaca tcgatatacc ccctggctga
    19861 actggggttg aactcgtcgc tattctaacc attgctttcc ttttcggcta tccactcggg
    19921 tattct
//
LOCUS       DAZGCQ010000091        19589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000091 DAZGCQ010000000
VERSION     DAZGCQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.091"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2060
                     /locus_tag="ACHK07_003667"
     CDS             <1..2060
                     /locus_tag="ACHK07_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8103949.1"
                     /translation="AEAVGAVRSTLGGLGAVSENELTRITRKMLDMQAVMGGDIPQHI
                     QAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYSTHFRNMGLTGSET
                     MTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGLDAARMSSAVASGG
                     TAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPAFLGALARVSDRMA
                     DVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRGLVRMATAWGERLQ
                     AWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLRLGLALLRAIPAGL
                     SVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLIWRYWDRVRAFVSGFIE
                     GVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTRDLLEQCTSAGQTF
                     GSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDEARKTPVAWEWDPE
                     QKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNVLRHLKGIEGNTGG
                     LLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALPQEEIQVQQWPTAR
                     PAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAEMNRMARAGTGSFR
                     DRD"
     gene            2071..2562
                     /locus_tag="ACHK07_003668"
     CDS             2071..2562
                     /locus_tag="ACHK07_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103950.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(2573..3160)
                     /locus_tag="ACHK07_003669"
     CDS             complement(2573..3160)
                     /locus_tag="ACHK07_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8103951.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(3172..4512)
                     /locus_tag="ACHK07_003670"
     CDS             complement(3172..4512)
                     /locus_tag="ACHK07_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103952.1"
                     /translation="MMAAKYKTIITNAGTAALAAAVLPDGEKITLTHIAVGDGNGKPT
                     EPLATQTALVKEVWRGEVSRVSRDAENTSFLTAEVIIPANEGGFWIREIGLFDSKGTL
                     IAVGNVAEGYKPQLDEGSGREQVFRMVILLSNEASVNLTADSSTTMATQAGVAEMIKQ
                     HEQNGKHPDATTTSKGFTQLSSETDSDDETRAATPKAIKALKALAVTRPTAALSVDLD
                     TLGRPEHHGDYYQPNNSGATTAHHYPVTSAGSLSIRMGAWGFCQQEYTAWNPPRKFIR
                     TVTGNFTGNGPWSEWKEVAILPLTQTGDASLTERGIVQLSSATDSDSEVLAATPKAVK
                     TAMTAAENAGQKARQALEAAQAKTQRTLWDVGSFILAATDAEGVYPARINIAPGDTRP
                     GSKLRLMSMTNSDFEISFYESMQNWNTNRRPPGTWRACADIYAGAGIGIFERIA"
     gene            complement(4512..5135)
                     /locus_tag="ACHK07_003671"
     CDS             complement(4512..5135)
                     /locus_tag="ACHK07_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO8103953.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGISRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(5128..6024)
                     /locus_tag="ACHK07_003672"
     CDS             complement(5128..6024)
                     /locus_tag="ACHK07_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO8103954.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARNALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(6011..6376)
                     /locus_tag="ACHK07_003673"
     CDS             complement(6011..6376)
                     /locus_tag="ACHK07_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO8103955.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(6373..6954)
                     /locus_tag="ACHK07_003674"
     CDS             complement(6373..6954)
                     /locus_tag="ACHK07_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8103956.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRVTSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKVRTFELTDGGTLKGDI
                     THSNGNLRSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(6951..7595)
                     /locus_tag="ACHK07_003675"
     CDS             complement(6951..7595)
                     /locus_tag="ACHK07_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8103957.1"
                     /translation="MMAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKR
                     IRSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRRLKNWRTTTGRYGPMITGYDTE
                     RRGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEG
                     RAARIARVHQFGEVSLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNAR"
     gene            complement(7579..8046)
                     /locus_tag="ACHK07_003676"
     CDS             complement(7579..8046)
                     /locus_tag="ACHK07_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103958.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(8062..8235)
                     /locus_tag="ACHK07_003677"
     CDS             complement(8062..8235)
                     /locus_tag="ACHK07_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8103959.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            complement(8382..10427)
                     /locus_tag="ACHK07_003678"
     CDS             complement(8382..10427)
                     /locus_tag="ACHK07_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8103960.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEEKPVPGTTDGYNTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAVPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAANRFAMALYWPVSQLPENVV
                     PEGSTGSNMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPASGVENDKLLITDITSNQDTYPVLIEYVTVDINNSNAA"
     gene            complement(10438..10569)
                     /locus_tag="ACHK07_003679"
     CDS             complement(10438..10569)
                     /locus_tag="ACHK07_003679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103961.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            complement(10556..10981)
                     /locus_tag="ACHK07_003680"
     CDS             complement(10556..10981)
                     /locus_tag="ACHK07_003680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO8103962.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDAGTGAAATHDMG"
     gene            complement(10978..11520)
                     /locus_tag="ACHK07_003681"
     CDS             complement(10978..11520)
                     /locus_tag="ACHK07_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8103963.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQARQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            complement(11507..11791)
                     /locus_tag="ACHK07_003682"
     CDS             complement(11507..11791)
                     /locus_tag="ACHK07_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8103964.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            complement(11794..11994)
                     /locus_tag="ACHK07_003683"
     CDS             complement(11794..11994)
                     /locus_tag="ACHK07_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO8103965.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            complement(11994..12488)
                     /locus_tag="ACHK07_003684"
     CDS             complement(11994..12488)
                     /locus_tag="ACHK07_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8103966.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            complement(12593..13390)
                     /gene="gpM"
                     /locus_tag="ACHK07_003685"
     CDS             complement(12593..13390)
                     /gene="gpM"
                     /locus_tag="ACHK07_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO8103967.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREITTLE
                     RAVRKLADGNQVVPTSTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            complement(13431..14531)
                     /locus_tag="ACHK07_003686"
     CDS             complement(13431..14531)
                     /locus_tag="ACHK07_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO8103968.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGPIASTNTTSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            complement(14549..15385)
                     /locus_tag="ACHK07_003687"
     CDS             complement(14549..15385)
                     /locus_tag="ACHK07_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8103969.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIEMLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDALSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            15541..17274
                     /locus_tag="ACHK07_003688"
     CDS             15541..17274
                     /locus_tag="ACHK07_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO8103970.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNDGQGEQAVSGKKP
                     RRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVAGAMATLEGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRY
                     TNIGVIRGENTESEFRNLYMCEFVRDGEAAFSLSALTGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            17280..18323
                     /locus_tag="ACHK07_003689"
     CDS             17280..18323
                     /locus_tag="ACHK07_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8103971.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(18389..18892)
                     /locus_tag="ACHK07_003690"
     CDS             complement(18389..18892)
                     /locus_tag="ACHK07_003690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103972.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKLQR
                     RTPSAFR"
BASE COUNT         3850 a         5313 c         5509 g         4917 t
ORIGIN      
        1 cggctgaagc tgtgggtgct gtccgctcca ctctgggtgg gctgggggcg gttagtgaga
       61 atgaactgac ccgaatcacc cgaaaaatgc tggatatgca ggccgtgatg gggggcgata
      121 tcccgcagca cattcaggcg gctgccatca tgatcaaaaa cggtcttgca cgcagcagtg
      181 acgaggcggt tgatctgatg gtggcgggca tgcagcgcat ggggccggtt atgcgcgatg
      241 agctgcctga gatccttcac gagtattcga cccattttcg taacatgggg ctgacagggt
      301 cggaaaccat gacgctcctt gcggatatgg cgcagcaggg gaagtttgcg ctggataaaa
      361 ccggcgatgc catcaaggag ttcagtattc gcggctccga tatgtcaaag gcgagtgctg
      421 aagcgtatgc gcttatcggg ctggatgcag ccaggatgtc atcggctgta gccagtggtg
      481 gaacggctgc acgggctgcg ctgcagaaaa cagcctcggg tctgttgcag atcgtcgatc
      541 cggctgagcg ggccaatgcg gccatcgcac tttttggtac accggttgaa gatctgtctg
      601 tcgatcaaat ccctgccttt cttggcgcgc tggcgcgggt gtctgaccgt atggctgacg
      661 tgtctggtgt ggcggaccgg atggggcaaa ccctgcgtga caatctgagt ggtgatgtgg
      721 cgcggcttac cgggacgttc agcgcgctga gaagtgggat tatcggggag attacggacg
      781 atctgcgtgg gcttgtccgg atggccacag cgtggggtga gcgtctgcag gcgtgggtga
      841 atgcgcatcc ggaactggtg cgaatgattg cgctgacgac gggagcggtg ctggcgttaa
      901 ccacagcgat gggggcgttg tccgttgcgg cctggatggt tgtcgcaccg ttactgaaac
      961 tgcgtctggg gcttgccctg cttcgcgcga tcccggccgg actctctgtt gccggtacgc
     1021 tgtttggcgg actgacgggt gtgatttcag ccgtgggggc tgcgctgggg gcgctgacct
     1081 ggccggttgt ggtggcggtg ggagccctgg tcggtggtgc tctgctgatc tggcgctact
     1141 gggatcgggt cagggcgttt gtgagcggat ttattgaagg ggtacagtca gcactggcgc
     1201 ccttcatggc gacagcctcc cgttttgcgc cgctgctgga tctggtttca ggggcactgt
     1261 cccgtattct gggatggttt aaatccctgt taacgccagt ctcctccacg cgtgatttgc
     1321 ttgagcagtg cacctctgcc gggcagacgt tcgggagcac tctggccggt gcgttagcca
     1381 cggttttttc gcccctgaaa gctctgattg acggtctggc gtgggtgctg gaaaaactcg
     1441 gtgttctgcc cggtgaagct gagcgggtta agcgaaaact ggatgaggcc cgtaaaacgc
     1501 cggtggcatg ggagtgggat ccggaacaga aaaaaatggt tcagaaggcg tggcactggt
     1561 cgcctcctgg tggggccagt gtgccttccg gcgcctccgg tacacagcct ccgccggtgg
     1621 cgctccctgc ggggatagcg ccggacggta accgtaacgt tctccgtcac ctgaaaggca
     1681 ttgaggggaa taccggtggt ctgctggctg aaaaccgtaa acgtatcgga cccggagata
     1741 ttgtgtttaa acatcttcca cgggcgctgg cggtccgggg gcagtggcat gaacctgtcg
     1801 ttgcgccgtc ttccggcgga atgcgcgcgc tgccacagga ggagattcag gtgcagcaat
     1861 ggccgacggc acgcccggcg ccacgaatgc accagcagga aaccttcgcc agcgcctttc
     1921 agggggagat acatgtgcat cttcacgggg tgagttcgca taatccccgg gaactggcgc
     1981 gcatggttgg tgacgctgtc agggcggaaa tgaacagaat ggcacgtgcc ggaaccggca
     2041 gcttccgcga tcgcgactga ggtgaaaaag atgatgatga tttatgggat gtttgtgttt
     2101 cagttaagta ccctgccgca tcagcaggtg gaacagagta aacgctggcg acacgtcagt
     2161 aatgaacggg ttaaccgttc atcctcctgg cagtatattg gctctggtga agatcacatc
     2221 acgatgtccg gtgtgctctg tccggaaatc accggcgggg aggtgtcact gactgccctg
     2281 aacaccctgg cctacacagg attaccctgg ccactgattg atggtgtcgg acagatttac
     2341 gggatgtatg tcattacgga aatcaggacc acccgtcagg agctggatcg ctatggcatg
     2401 gcccgcaaaa ttgagttcac tgtcagcttt cagcgatgca atgaagatct gcgtgagcgt
     2461 cttcagtcgg gctcggtggg tgatctgatg gcaggattaa aaaacagcgc gtcgtcggcc
     2521 ctgtcgtcgg cgtcctctgc gctgtctggt ctcatgttgt gaaggtcact gttcaggctt
     2581 cggcttcttt tccccagcca accttatagt ccagaatcgc cgccgtatcg ttcagggcgg
     2641 cgatctcttt tttcatgtta cgctggcgaa tgtgaatttc catccctttg gcaaacaggg
     2701 cttttgcggc tgcatcgctg agggcaatca actcatcagc agaaacagga acatcgttgt
     2761 tctgtgcatc agtccagaaa aaatccagcg gcaggatccc cgcttttgcg gcagcaacgg
     2821 aaggctcaag gcgggcctgc gttgacttcc cgtagtccca tttcctgccg ttatgttcaa
     2881 aggtgtaact cgccgcttcc atcgcattac gccaggcgtt gatttcgtcg tttttcaggc
     2941 gtctggcgtt ttccctgtca gtgtcgtttt cctggtattc agatacaggg ccgtgatttc
     3001 ctgctttcag ttcgccccag agttttttgc cgtgtggcga gttgtcatat tcggttgccg
     3061 tatagggcag ccagctctcc tccccttcaa actggatctc acagtcgata gtgccgtcac
     3121 ggttataagc ggcatttctt gctgatttga ttgtgtgcat gagtctctcc gtcaggcaat
     3181 gcgctcaaat attccgattc ctgcgcctgc ataaatgtca gcgcaggccc gccacgtgcc
     3241 cggtggcctg cggtttgtgt tccagttttg catggattca taaaaactga tttcgaagtc
     3301 ggaattagtc atgctcatca gtctgagttt actgcccggg cgggtatctc cgggcgctat
     3361 attaattctg gcaggatata ccccctcggc atccgttgcc gccagaataa agctgcccac
     3421 atcccagagt gtccgctgtg tttttgcctg tgcagcctcc agtgcttgcc gtgctttctg
     3481 cccggcgttt tctgctgccg tcatggccgt cttcacagct tttggtgtgg cagccagcac
     3541 ttcactgtca ctgtccgttg cgctgcttag ctgaacaata cctcgctctg tcagggaggc
     3601 atcgccggtc tgcgtgaggg ggagtatggc aacctccttc cattcagacc acggcccgtt
     3661 gccggtgaag ttacctgtca ctgtccggat aaattttcgt ggaggattcc aggcggtgta
     3721 ttcctgctga cagaatcccc atgcgcccat cctgatggat aatgatccgg ctgatgttac
     3781 cgggtagtga tgcgcagtgg tggctccgct gttgttgggc tgatagtagt cgccatgatg
     3841 ttcaggtctt cccagtgtat ccaggtccac gctgagggcg gctgttggtc ttgttactgc
     3901 cagcgcctta agtgctttta ttgctttggg tgtggctgcc cgggtttcat catcactgtc
     3961 ggtttcacta ctgagctgag taaatccctt actggtggtt gtggcatccg ggtgtttacc
     4021 gttttgttca tgctgcttta tcatttctgc cacgccagcc tgtgttgcca tggtggttga
     4081 tgagtcggct gtcagattta cagaggcttc gttgctcagc agaatcacca tgcggaatac
     4141 ctgttctctg cctgagccct catcaagctg cggtttgtaa ccttccgcaa cattacccac
     4201 ggcgatgagt gtgcctttgc tgtcaaacag accgatttcg cggatccaga atcctccttc
     4261 attcgcagga atgatcactt cggcggttaa aaaactggtg ttttccgcgt cacggctgac
     4321 cctgctgacc tcgccccgcc agacctcttt aacgagagcg gtttgcgtgg ccagtggttc
     4381 tgtgggttta ccgttaccgt ctccgacggc gatatgtgtc agcgtgatct tttctccatc
     4441 cggtaacacc gcagctgcga gggctgctgt tccggcattt gtgataatgg ttttgtattt
     4501 tgcggccatc attactcctt gtccggtctg atggttactg tgatgccttc ataggtgact
     4561 ccacccgggt ggatgtcacc actgactgac agcgggcttg tcagttctgc tgccggatat
     4621 accacgatgg tttccccggt cagcgtggcc ccggttatgc gggatatacc ccgtgcaacg
     4681 taggtcattt tcatggcggc aaggtgtcgg cttaccgggc gaacggtggt aatcattcgc
     4741 tccatctcct gccgggtttc ctctgtcagt ccgcgctccg atatgtcaat atccagcctg
     4801 aatgaccccg gcggatcgtt gttctcccac cattctgtaa tactcagtat cgcgccgaag
     4861 ggctccacag cccgacgcat ggcgctgatg gtgcctttgt ggcggtgaat atgccacgag
     4921 tcacggatga tctgacgacg tgcctcttca ctccatgttt catcccagcg gtcaacggaa
     4981 agcgcccagg caagccatgg cagcagatgg gctgggcagg tgtcggggtt ccagagggtg
     5041 ttgatatgga tcggcagctc tgacaggcgc tgagcgccct gttccgaatg atgcaggaag
     5101 gagggcgagg atggcggcag cagtcggtta ttcatcggtg ccacctgcag agatgttccg
     5161 ggatgtgcag cgggctgact gggtgttact gatcaccaca ttctgcgctg gtttcttcag
     5221 ctcgactcgc tgaacgcccg gtacgtgtag cgttgccata atggcagagc gggccacatc
     5281 acgtcctatg gcattctgag ttttcagcca tgtgtcaagt gcatttctgg cggcggcaag
     5341 aatgggttcg gattccgggc ccggataaaa atagagtacg gcctcaatat cgtaactgac
     5401 gatggtcgca ctctgtaccg tgagtctgtc ggccaggggg cgcacagttt catcattgag
     5461 agcccggctg acggtcgcga ttaattccgg tgacgctgtg ccgtctcctt ccgttgagag
     5521 aatggagaca atgaccgtcg caggtgacgg actggttgcc cgtgcatcgg caacaagacc
     5581 gctggcactg cgggcaaaat attcatatgc agcagagggg cctgcgacgc tgaaaccttc
     5641 aaatccggat tgtgcccgca gacggaacgc gctgtcgctc tccatgatgg cagcttctgt
     5701 atcagtagca ggtttcagtt caaggcgttt cgcattcacg atagcggcga gattatccag
     5761 gtctgtggat acggcatggc tgagcatgca ccccgccgct gcatcgttga tccgctgacg
     5821 gagcagcatt tcgcgaaagg cgatggcctg agcaatgatg ttgagtggct ctgattccag
     5881 cgcaatggct gcgctgacgg cactgcgcat gttttcaggg aaggcatcaa tcataaaggc
     5941 cttcacgtct ttcagtatgc tctcataatc cagtgattca atgatctgcg gacgtggtaa
     6001 ctgagacagg tcaattgtcg gcattactcc ccctcagcgg aatgctcatc tgtacgggct
     6061 gcattgtgcg ggtgagcacg cctgagatgg ccagcagagc gcgtccgtca ggcagccagg
     6121 cgatatccac ccggttaatg gcgatccggg gctcccactg tgcgagcgcc ataaccaccg
     6181 cgctcatgca ctgcagacgc gtcacgtcgt tttgtggcgc atcgagcagt tcaggtaaca
     6241 ggctgccata ctctcttcgc atgatgcgac tgccgcgcgg ggtggtcaga atattgttta
     6301 cagagtggcg gaggtgttct gtgtcactga tgcatccgct accatgcggg ttcatgcctg
     6361 tgcataccgg tgtcatttcg gcccccctgt catgcttccg ccggactgaa caccgttatg
     6421 aacgtgcgta tgcacggtaa caccatttga gcgtaagttt ccgttactgt gtgtgatatc
     6481 ccctttcagg gtgccaccgt cagtcagttc aaacgtcctg accttcagat gttttgtgca
     6541 ctctacggcc ggtgtgtcca gggtaatacg tgtttccgca gagatctctg cggttttgat
     6601 tcctttcacc gtcagcgcgc ctttttctgc gtcatagcgt atctcggcac cgtcaggtgc
     6661 aaggagtatc acttcattct gttttgtgcc gggcgccggg tgttcttcgc tgtagagact
     6721 gccagccaca acggccattt caggactgcc gcccgggcag agcaacagca cctgttcacc
     6781 gggggagggg ggcagccaga ttttaaacgc cccggcgcgt gtgcagttcc agcggagcca
     6841 gtccgttgtc agtccgccgc tggtaacgcg aatgcgccag tttttcagat ccacctccga
     6901 aacgacgccg gtgcggacca tgttttccag cagtcgcatg atttcagcat tcaacgggcg
     6961 tttctccaca ggttgttgat aatgatttcc gtcactttct gtctgtcggc ttcactgaat
     7021 cccagcagtt cgcgttgcgg atagcggacg gcattaccgg cagagaccag tgacacttcg
     7081 ccgaactgat gaacgcgggc aatgcgggct gcgcggccct caaatcccac gctgacgccg
     7141 ccgggatcgg tttttacctt cagaaagcgg tttgtccgaa gctgctcaaa catgggggga
     7201 cgcttaatgg cgctctgcgt tcgcctgcgg gtgtcaattt ccaggtattc ttcaatatcg
     7261 gctttgtgga acgagcgtat tccccggcgc tccgtgtcgt aaccggtgat catggggccg
     7321 taccggccgg ttgttgtccg ccagtttttc agtcgtctca cctgagtgcc gttccagatg
     7381 aacaccacac ctgcctggac acggaataca cgttttttac gggcgggcca ccggttaccg
     7441 tccggtgatt gttgcgaacg tatacgcttt tgttgcgcgg cgcggacagc ctgaccgaca
     7501 gcacgggctg ttttgtttat accggcagcg gacagaccac tgaggatctc ctgaaagaga
     7561 ccgtccagtc tgtgcaggtc atccggagcc atcattctga ccccatgtga catcttcgag
     7621 ggtgacgcgc catgcttcca gggcccggct gacaggcggc tctccgatgt gttctgcgtg
     7681 aacagtgcca tcatcattac ggcggataat cacccgctcc cataccggga tttccagcag
     7741 gatatcggtg ctgtcgtcgt cattgatggc aacagaaaat ttgatatccc tgtttttctc
     7801 cggattaagc aggagatcag gctgattgcg gtacagccag cgcatcagcg gcaccatcag
     7861 ttcatcgaga tcctgcgtga agtccatgac aaagatccca agcgtgtaac ggtaaagata
     7921 tgagggggat tcgccggtcg tctcaatgcc tccgttattc acatacacag tgaatttctc
     7981 cgggttggcg cgacaccatg ggcaggcgtc agtcatggcg tcgcgcaggg tgttaatttt
     8041 cagcatgccc cctcctgtgt gttatggcat gttctggttg ttctgttgtg cagacttctg
     8101 actgattttt tcagcggcgc gctcgtcaat ttttcgctga aaataatttc tgataacact
     8161 gtaaccgccg ctgatcagat acagcgcgca gacggcagta cagaaataca gcatgatctg
     8221 atgaatgaat aacataatca cctcacacaa atgattgaca taacgatgtc tgtcgtttta
     8281 aaaaaaatga ataacaataa taaacactct ccataattcc tcttgcgctc ccgtcaggta
     8341 tcttctcctc tcagtcttgc ctgccgggag cctctttttt cttatgcggc gttgctgtta
     8401 ttgatatcca ccgtcacata ctcaatcagc accgggtagg tgtcctggtt actggtgata
     8461 tccgtgatca gcagtttgtc gttttcaaca cctgatgccg ggcatttcgc cagattaaag
     8521 gccgcctgtg tctgaccgtt gatgaccggt gtcacgctga tgctgttatt gccggcaaac
     8581 cggaacacca cgttgtgcca gttatgatta aatgccccga aggttcccag ttttgcattc
     8641 tgttgtttgt gctgcatcag attcaggttt gcgctgtcgc tctggatgaa gaaggctgcc
     8701 agcatattgc ttcctgtgct tccctccgga acaacgtttt caggtagctg agaaaccggc
     8761 cagtacagag ccatggcgaa gcggtttgct gccagctgaa caccttccgg gattttgaaa
     8821 cgaaaggcaa tttccccgcc tttctgaagt aattcagctg ctttacttgc aggcacatcg
     8881 tgggcgattt tccaggctgt ttgttgcccc tggacttttt ccactcgcat ggctttaccg
     8941 ctctcagcag cagagtcatt tacgacggag gcttttccat ctgcccctgt ccagccctgt
     9001 tctttcagtt tcccttcctg tgcgtctgcg cgataagcga cggccgttgt tcttgccggt
     9061 gatgactgcg tacctgcgcc cggctgaggt gcaggtgtgg agggctgcgg gctttccgtg
     9121 gtggtgctgt ctgaaccggc cgtggcggga ggtactgcga ccacgctgaa cggtgcgggt
     9181 gcacctttca ggaaggcgac ggtcttcccg gcatgcatca ggatcgccgt tgccaggcgg
     9241 tcagaaataa tgccgcggcg ggcccatgaa ctgaaatgcg tggcgcgatc tgaagaggtc
     9301 cagtttccgt tactacggga tgcagcaccg taataaccgg cttctgccag gtcaggatct
     9361 tcggcaggca ggtttgttgg cgtgttagca cctttctcat cggtcatcag gggcacaaaa
     9421 taaatttttt gcgcttcttt gttctgatat gcaccgtaaa ccaccgggta ctgagaggta
     9481 tattgttgtt tccagatata ggtggtatcg ccgcaaatcc agggaacaag tgatgcgttg
     9541 cctccggtgc actgtgcagc atatgcgctg agatcggcgc ggaactgatt caccatgttc
     9601 aggaataatt gcgggtgctg tgcgtatgtt gctgatttca gatcaaactc tcccagcatc
     9661 cagaccacgg ataacaggac gtttttgggg tttttatcca gcgcggcttt tgtacgggta
     9721 agcagatcgt tatacagcgg tttgccgacg ccccacaggg atgattcggc cgttgctcct
     9781 gaagattcac tgaatgcccc ctctgcgccg gaagtaaacg ctgaccctcc acgacagcaa
     9841 ggtaccagga ggatcccggc atttttcggg ataaacgcca gtaatttttt ggcaatatgt
     9901 aaaccctgtc cgacgcagcc atactgacct tttgacagat cggcgttcgg atgattaagg
     9961 ctgctcatat cctgtacatc atggagacag tgatctgccg gaataatatc gttataggta
    10021 catgcagcgc cgcccggtgt caccgtactg cgacgcgcta actgtttaat tcgtggatca
    10081 ggactgtcga atgtatccgg taatggcagc ccctcaccgt atgccatgcc attggactga
    10141 ccagccagtg gcaccacata ataaaactct ggctcagttg tgtgtccagt gttgtatccg
    10201 tcggtagtgc ccggtacggg tttttcctcg ccttctggta ccgggatggc tgatggcagg
    10261 tctctgtcag tgaccagtgc ctgcatcagg taaaatggtg ctcccggctc tgatgctgag
    10321 acaggtcccc caagaagtcc ccatccttcc ctgagcatgg gcgcaagttt ttcggcaagt
    10381 tccttgttcg tcattgccgt cacaagctga taatgtttcg gtaccatagt gactccgtta
    10441 tttttcatga gtgttcattc gtgctgtccg ctgccgctcc agcccggcaa tggcggcttt
    10501 atcggcatta caactgtcca gggcatctac cagtcggtct ccccagtcag ccacatcagc
    10561 ccatatcatg tgttgcggca gcaccggtac cggcgtctgt ctggtcagac tttccggcac
    10621 gctcccgcaa atgacgccgg ttttcagcgg cgctggcgtg ttgttgcagg ctgtcagcaa
    10681 catcagcagg gggagaagtg cgtgcgcact catccgcctg taagtttttc cgcatcgttt
    10741 cacgtcgttc ttcccctctg gctgcacgct cctgatcttc gcgccggata gcggccagcg
    10801 ctgactgtgt ctccgccgtg gccttaagca actcccggac ggtcgtattg ctctctttca
    10861 cggctttttc cagcgcttca taacgggtgc tctcctgacc tgagcgatag gcccggatga
    10921 ccaccattgc ctgcagcgaa atggtcagca gcagcgcaca aaggataatg ttcagtttca
    10981 tggctgcgct cccgcgtctt tcaggcacca gttcttaaaa tcattccggc ggttaaccag
    11041 tccctgcgtg cgcgttccgc cactgttcac aaaatctgtc agccggtaac acatgaggcg
    11101 ccattgccgt gcctgcgcgt gtttccagat ggtggtgcgt tgcggcgtgc cgtttttgtt
    11161 tttaaaccac atcagattcc ggcagcccag gttaaaggcg gcgtccgtca tggcttcgaa
    11221 tgcagactgc ggcatctgct gccccctgaa attctgtgtg atgcagttct cagcgcggcg
    11281 catatcgttg atccaccgcc cggccacttc ctcctgtgtg tacaggcggt tttccacgtt
    11341 accggtggat ccgcatccga ccgtaccgac ccccaccaga tcacggtagg gtcgggcccg
    11401 gcactcctcc catgtggcaa ttttgatttg tgcctcgcgg gaggttctca gttctccggg
    11461 agccagggag atccccagcg ccacaatggc ggccacggcg taacgcttag gtccggccat
    11521 cgtgctcatc ctgcttcaga agcatcattg cccggcgctc tgcgtcgttc agctcccttc
    11581 cttctgactg cataaggatt ttttcaatca gttcgttacg ccttgcctgg gcgatctcca
    11641 tcctgcggcg gtgaagccag gcacgaagca gtgacagcgt ccccagcaca aaaccggcca
    11701 cggcaatttt ttcgctcagc gtcatcacgc cgataccggc tgtcgtcact gacgcccaga
    11761 acgtgatgtg atcacaaagc cgctgaaaca ttctcacccc cacaactgga tcatttcacg
    11821 ccgggtctgc ccctgcagct ccggcagctc cacttcctgt ccggcatgca gaaacatgac
    11881 ggctgaaatc cccggattgg cggccagtac cgcctcagtc accccctgcg tggtgccgta
    11941 atggcgctga cagagtaaat cgacggtgtc accgtccaga gccattactt tcatcagacc
    12001 agctccgcat aaatgcgggt gacaccgcgt atgtctgata ttgcccagcg ggcatcccgc
    12061 cacaaatcct caacctgcat gtccagcgcg tcagccctgc ggtcgccttt atcggtggta
    12121 tccacatccc gggtgcgctc cagtacaaac gcgcgggtga gtgcgaagac ggccctgcgg
    12181 tagcggtgga cgtgtacact ctctccgttc acctgcgtgg ccggaacgtc agccagtgag
    12241 gccgcgcctt cagccatccg ctcacgtttc cagagctcca gttcggcgat cacatgggcg
    12301 accgcttccg ttgctgcatg taacagacgg gttgttgttg tcctccccgg aatgcgggaa
    12361 gccaggcgaa tttcatgcag tgagatcgcc ggccagaagt cacccgcgct caccatggcg
    12421 tcgccgtcat cggtgtcggt ggtatctgtg tcagcgctgt tcacgcgggg tctggctatc
    12481 agactcatgc ctgtctccgt aaaaatcagg cggtgggcaa ccggtaaaaa gaacgcgaag
    12541 cctgttcaga tcaccggttg cgccgcctgc cgacgaggtg cggttcgtta agtcagtttg
    12601 ttttgcgcgc ggcttttttc tggctggcag cgcttttctt ttttgcgctg ctctttttcc
    12661 cgccggtggt ggttttcctg cccgttgtgg atgtaggcac cacctgattt ccgtctgcca
    12721 gtttcctgac cgcgcgctcc agcgtggtaa tttcacgctt cactcccgaa gccggattga
    12781 tccgcatggc ttcacgtaac aggctcagcg acagacgcag atcatcggga gaggaggagg
    12841 cgcggcggga aaatgcgcgt gccttgtgca gttttgacct gacctggtcc ggcatatcct
    12901 gctcactgac caggtggttc agctcatcaa gcatggcggt gtacacagac aaatccgcct
    12961 cgctgtccgt tgtggcctgc gtcagaacgg cgtcagtaat ctcttcagcc agtaccgtgg
    13021 cggcgtcacg gccaaagtta tccggcagcg ccagaccatg gcggatcacg tactccccga
    13081 tacgcagtgc cagcgggtag tcacggcagt caatggccca caccatcatg gtggtgatga
    13141 cctcatcctg gcgtccgctg tctcctgcca gggtgccttc aatccaccct tcaaagtcag
    13201 gcaacagatg ttttttaagc tctgccctgg cgacgtttga ctgaataccc ttcagctgca
    13261 tctgtgccag ccttaactgg tgaaggacct gttcatgtgc ggttctttcc cgttgtgttt
    13321 cccgggcgtt catggtcacc cggttgcggg ccaccgcctg ttgcacatag cgttgtgccg
    13381 gtgtcagcat ttctcagctc ctgttaacgg gcgcggcgcg cccgtccggg ttatgcttcg
    13441 ggttgcggct gtccgctttc ggcgttgttg tccggatctt cacctgcgaa ggtgatcccc
    13501 tcaatcagcg cgcattttcc gtagtcttcc accacgtagc agtcattcat ggactggtaa
    13561 gtcgcgatac ggttgtattc cggctcctcg cgaatgaaac ggcgcagtga gcctttctgg
    13621 aagtagatcg acaggttttt cagcgacgtg atgagcatgg cgtctttcgg gaagtacggc
    13681 gcaagatacg tctgaagacc accgacggta cgggacgcca caatcagctg cccggcaaca
    13741 agctcagtgt tcgggttggt ggtgctgatg gcgttaatca tgggaagacg cagtgtattg
    13801 aacagatccc gccccatcag tacgaccaga tccggtgcgt ccttatgcca ttcatccagc
    13861 acggatgagc ggatgtcgtg caccatggca tccgggttac catagtcacc ttttgccacc
    13921 agtttgtttt catcatcgcg acgggtcagg gtcatccctt tcaccacgcg ggtggcagcc
    13981 ttgtcgcgga tgtgtttcag ccagcccggg ttgacgtcct gcaggagagg gttggtggca
    14041 cgatcggaaa tcagcgcgtg tgactccccg ttgaaaccaa tcatgattcg atccagggct
    14101 atctggcggg tgatttgcgc gctcagcagt tgctgaaagt ttttctggct gctccatgcg
    14161 tcaatctgtg cgtagctgat gaaggtatcg tagttcacct gctcgcagcg gtaacggcgt
    14221 gcatccagat cagcaactga tgcaggattg cggcgtttca ctccgtcgct cgttgtgttc
    14281 gtgctggcaa tggggccgct ggtgtcaatc aggacttttt caccttcctg gtcatccaca
    14341 ccgataaaat tgatttcctg taacagtccg gcgctttcct tgatggcgtt ctccatcttc
    14401 tgctgaatgg atggatccac gctgaaggtt ttacccaggc gctctgttgt gataccagcc
    14461 agcgatgcct gctgaatcat atagcgttcc agactgtcac gtccttcacg ggaaagggat
    14521 aaacgggcca taaattactc ttctcctgtc agaattcagc cagtgtctgg tcagtgccgc
    14581 cagcggaagt aaaacgctgc tgtggatcgg catcctggtt tctcagttgc gctttcagtg
    14641 ctgcaagctc gtgactgagc gcatccagcg ccgttttgtc gagggcatgt ttctgctcca
    14701 gagtgctgaa acggtcgata agatctgcat gatcggatgc gatgttttcc acaacgtcgc
    14761 gcagctggct gaactgctcg ccatccgctg tgcgtgtttt gctgatgagt gccatgacgc
    14821 ggctgaacca ctgacggctc tcttccgttc gctgttctgt ggtggtaagc atctctgctt
    14881 ccatggcgac ggtgaacatg gggggttctg ttgtgcactt actgaactgc atgatgctgg
    14941 cgcgttgctg tgcggcaaac tccaggcgtt cagtgcccag acttgctggc gtgtcggtca
    15001 ttgccagacc cacaatgtaa ggccccttga cggcgtccag ctcaggcaac atctcaatgc
    15061 tcgaatacac ttttttcccg tcagcaatca tgtttttcat gcggtcggtg ggttcaattt
    15121 cggcatataa cgctgtacgc cctgccagtt tcccttcggt gatttcatca gttgacagag
    15181 cgatcagatc acccactgcg cagagttcac tggatggcca catcgaaagg acgtgctcaa
    15241 tgttcacccg ggaggcgtaa acggacgggt catagttttc tgcggcctga cgcagcattt
    15301 caccagaaat ttcacgtccg tcgatggttg aaccggaaac ggcaacgcga aattttttgc
    15361 gctctggcgt gtttttgctt cccatggaaa ttctccataa cctggtcgtt tttcccatga
    15421 tggcggcaga taacggtgtg tctcaaggtg cttaagttgt atggcgacgg ggacaacgcg
    15481 ggctggtatc aggcggtatt gctgaggctt aatcttgccc tgttaatgtc agggggtgca
    15541 atgatccagg atgtctttat tcgtatgcgg gcgaaacagc tttactggca ggggtatacc
    15601 ccggcggata tcgctcagct gatgggaata aaccagaaca cgatttattc gtggaaaaaa
    15661 cgcgatgagt gggatgccac tcccccgatc cagcgggtca cccagtccat tgatgcacgc
    15721 ctgatacagc tcaccgccaa accggataaa agcggcggtg actttaagga aattgacctg
    15781 ctgacgcgcc agctgaaaaa actgaatgac ggtcagggag aacaggctgt ttccggtaag
    15841 aagccccgca ggcgcaaact gaaaaatcac ttcaccgaag agcagattgt cgcactcagg
    15901 gaaaaaatcc tgccgtcact ggcctggcat cagcgcgaat ggtacgacaa ccgccatcac
    15961 cgcaaccgca tgatcctgaa gtcgcgtcag attggtgcga cctggtattt tgcgcgcgaa
    16021 gcactccttg atgcactgcg tgatgatgtc tccgccgggt accagcgcaa ccagatattt
    16081 ttgtccgcat cccgtcgcca ggcgtatcag ttcaaacact ttattcagca ggcggcgctg
    16141 gaagttgatg tcgaactgaa agggggtgac aagattgttt tgtctaacgg tgcagagctt
    16201 cacttccttg gcacatcggc agcaaccgct cagtcgtata ccggacacct taaatttgat
    16261 gaattttttt gggtcagtaa ctttgtggaa ctcaggaagg ttgctggggc aatggcgaca
    16321 ctggaagggc ttacgcgaac ctacttttca acaccgtcag gtgaaacaca tgaggcgtat
    16381 cagttctgga cgggggaccg ctggaacagc gaacagccac gctcaaaacg cagtgagttt
    16441 gatgtttcac atgcgcatct gaagggggga gtgctctttc ctgacgaaac atggcggcag
    16501 attgtcacta ttgaggatgc tgtcgctaac ggatggcggt acaccaatat tggtgttatt
    16561 cgcggcgaaa atacagaaag tgaatttcgt aatctgtaca tgtgcgaatt tgtccgtgat
    16621 ggtgaggcag ccttcagcct gagtgcgctc accggctgcg gtgttgacgg ctatgacgaa
    16681 tggcctgact ggaaaccttt tgctgcccgc cctatggggg tgagggaggt ctggcttggg
    16741 tatgacgcca acggcagcag cgggaagggg gactgcgggg cactgtcggt gtgcgtgccg
    16801 ccccttgttg ccggtgggcg ttttcgtacc gtggagacgg tcagggtgca gggtatggag
    16861 tttgaacagc aggccgcact gattgaggaa tacacccaaa aatataacgt gaaacatatc
    16921 ggcattgatg tgaccggcgg gaacggggag ggggtttatc agattgtgaa aaagtttttc
    16981 ccgatggccg taccgtacaa cttcaatatc gcctccaagc gggcactggt catgaaaatg
    17041 cttcaggtgg tgcgtgccgg acgctgggag tatgaccgca gtgaacgtga cctgatccag
    17101 gcattcgcat cggtgcgaaa aatacagaca cccggcggct atatcaccta tgacaccgac
    17161 cgctcccgcg gtgtgagtca tggggatctt gcctgggcaa ccatgcttgc tgttatcaat
    17221 gagccaattg gtgcggagcg tgaggataat cgcggctttg caatggagtt ctgaacacaa
    17281 tgagtaaacg caaaaaatac accggaaagc gggggcagga ttcgctacac gatgcactga
    17341 aatcaacgcc tgcactcagc gcattcacct ttgatgggcc ctatgaagtc agccgcatgg
    17401 atctcctgga taacatgtca tgtgcagata acgggcgcta ctttgatacc cccatggact
    17461 ggaacgctat cgcccgtgca tcccgtcgtg cgtcctggca tcagtcggct ttgtacttca
    17521 agcgcaacgc cctgaacggg tgctttgttc ctcaccctct gctgtcccgc caggcgtttt
    17581 cagcgtttgc gctcgactgg tttgtgtttg gtaatgccta tcttgaagtg cggcgtaaca
    17641 ggctggggga accactcctg cttcgcccgg cactggcaaa atatacacgc aggggaagtg
    17701 acctggatac ctactggtat ctcaacgacg acggtacgga gttcagtttc aggaaaggga
    17761 ctgtgtgcca cgtgcttaat cctgatatta atcaggagat ctacgggatg ccggaatata
    17821 tcggcgggtt gctgtctgtc agcctgtcaa actcagcgga tacattccgc aaactctact
    17881 acgataacgg ctcgcatgcc gggtgtatta tctatgtagg cacacctcag gcgaatgcgg
    17941 aaagtgtcga ggccattaaa aaaacgctta cggattcccg tggtcgcggg gcgtttaaaa
    18001 acctgtttct acatgcgccg ggtggtggca aggatggtgt gcaaatactg ccgttccagc
    18061 agataaccgc aaaggatgaa ttcctgaaca tcaaggggag cgcccgcgac gatatccttg
    18121 ctgcccatcg tgtaccacct caactgatgg gggcaatgcc tgacgggaat gcggcgtttg
    18181 gtgacgttga aaaagcggcg agagtgtttt tcatcaacga gttgcaacct gtcatggagg
    18241 ccatgaagca tgtcaatgaa tggcttggcg ttgaggtgat gcgctttaac ccctatgccc
    18301 tgcttctgga taacgccagc tgacgcgctt cgcttgtctg ctgcttcgcc ggggcataaa
    18361 aaatttatgc cccgactctc cagctctctt aacggaatgc ggatggcgtt cttctttgca
    18421 gcttgctgat aatatgcatc ttcatgctgt ttactatcgg cagccattcc ctgtcaccgt
    18481 ctccgttgat gaccacgcga atggaggtat catcttccac gcgaacccga agagatacac
    18541 cgtcttttcc gacggaatgt aactccattg ccaggactat gtttaccgac tctccttcat
    18601 cgtgtgagtc aacccatgtt cttacagcca tccggaatat gttatctgtg tcggttgcaa
    18661 tcatatttac cggaaatggc ctgaagtccc gatcgtttct gcacggaaac cccgtatcaa
    18721 cgtaacgatg atgctcgccg tctttgtctt cccatgtctc tgaaggcaat tccagccact
    18781 gctcataaaa agcaaccagc ttttcagcct gattgcgcag aatccctgta acctcccgcc
    18841 tcgcgtcccg gctctttttg tttgccaggt acaaatcgcg aaaatggctc atgtcgctct
    18901 cctttggtct gtttatgcca gcctgtattc tgcgctcacc tcatattgtg cgcaagtttt
    18961 cacccgtgaa tgactgatac tttaaattct attaacttta tgatttcaat gatttttgtc
    19021 tctctttttt atcccgttcg tcacgtccgt ttccctcccg atctccgacc tcgccagacg
    19081 caatcagacg cattctgacg gcctgtttcc ctgaccatgt tttgatgtgg tttttcaaaa
    19141 ctgactttac tgcgtgcgta tcacgcgcaa tacgacccca aataaagcaa cccgccttcc
    19201 atccctcaat tcgggcgccc ttccctcgtc gcctgcgcgc agcgatcgcc tcgtttttcg
    19261 tgcaccgtgc aaagtggctt cagccagcgc cgttactggg ctgaaagggt aaaaaacaca
    19321 taagaaaaat tgtgcaattt cgtgcacttt tgtgcggttt tccaggtggt gtttatgcgg
    19381 gaatcgcagg ctaaaaaaag catgagcggg gattgccccc gcgatattca tgccgcccga
    19441 tgagcttcac ctactgacgg gtctattatg atttcaaaat ccgtgaacaa cgctttcggc
    19501 tgtacgcagt taccttgttt ttccaggctg acaaagccgt atcgactcag catttttaac
    19561 gttgctgaca aattacttaa ctgccttcc
//
LOCUS       DAZGCQ010000092        19324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000092 DAZGCQ010000000
VERSION     DAZGCQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.092"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..325)
                     /locus_tag="ACHK07_003691"
     CDS             complement(<1..325)
                     /locus_tag="ACHK07_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8103973.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIK"
     gene            691..2013
                     /gene="xylA"
                     /locus_tag="ACHK07_003692"
     CDS             691..2013
                     /gene="xylA"
                     /locus_tag="ACHK07_003692"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO8103974.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            2085..3539
                     /gene="xylB"
                     /locus_tag="ACHK07_003693"
     CDS             2085..3539
                     /gene="xylB"
                     /locus_tag="ACHK07_003693"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO8103975.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            3708..4049
                     /gene="yiaB"
                     /locus_tag="ACHK07_003694"
     CDS             3708..4049
                     /gene="yiaB"
                     /locus_tag="ACHK07_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO8103976.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFFGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            4095..4532
                     /gene="yiaA"
                     /locus_tag="ACHK07_003695"
     CDS             4095..4532
                     /gene="yiaA"
                     /locus_tag="ACHK07_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO8103977.1"
                     /translation="MDNKISTYSLAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4574..5569)
                     /gene="wecH"
                     /locus_tag="ACHK07_003696"
     CDS             complement(4574..5569)
                     /gene="wecH"
                     /locus_tag="ACHK07_003696"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO8103978.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            5744..6043
                     /gene="ysaB"
                     /locus_tag="ACHK07_003697"
     CDS             5744..6043
                     /gene="ysaB"
                     /locus_tag="ACHK07_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO8103979.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            6138..7049
                     /gene="glyQ"
                     /locus_tag="ACHK07_003698"
     CDS             6138..7049
                     /gene="glyQ"
                     /locus_tag="ACHK07_003698"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO8103980.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            7059..9128
                     /gene="glyS"
                     /locus_tag="ACHK07_003699"
     CDS             7059..9128
                     /gene="glyS"
                     /locus_tag="ACHK07_003699"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO8103981.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            9450..9602
                     /gene="hokA"
                     /locus_tag="ACHK07_003700"
     CDS             9450..9602
                     /gene="hokA"
                     /locus_tag="ACHK07_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO8103982.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(9579..9650)
                     /locus_tag="ACHK07_003701"
     CDS             complement(9579..9650)
                     /locus_tag="ACHK07_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8103983.1"
                     /translation="MRNAVRKAGITSRRRLLLFQIAG"
     gene            complement(9790..10002)
                     /gene="cspA"
                     /locus_tag="ACHK07_003702"
     CDS             complement(9790..10002)
                     /gene="cspA"
                     /locus_tag="ACHK07_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO8103984.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(10283..10573)
                     /gene="yiaG"
                     /locus_tag="ACHK07_003703"
     CDS             complement(10283..10573)
                     /gene="yiaG"
                     /locus_tag="ACHK07_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO8103985.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            11007..11717
                     /gene="yiaF"
                     /locus_tag="ACHK07_003704"
     CDS             11007..11717
                     /gene="yiaF"
                     /locus_tag="ACHK07_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO8103986.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(11767..12741)
                     /gene="ghrB"
                     /locus_tag="ACHK07_003705"
     CDS             complement(11767..12741)
                     /gene="ghrB"
                     /locus_tag="ACHK07_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO8103987.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALMLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALLGKVEKNCVNPHVAD"
     gene            complement(12845..13504)
                     /gene="yiaD"
                     /locus_tag="ACHK07_003706"
     CDS             complement(12845..13504)
                     /gene="yiaD"
                     /locus_tag="ACHK07_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO8103988.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            13657..15990
                     /gene="bisC"
                     /locus_tag="ACHK07_003707"
     CDS             13657..15990
                     /gene="bisC"
                     /locus_tag="ACHK07_003707"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO8103989.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATEMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(15959..16399)
                     /gene="yiaC"
                     /locus_tag="ACHK07_003708"
     CDS             complement(15959..16399)
                     /gene="yiaC"
                     /locus_tag="ACHK07_003708"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8103990.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(16396..16959)
                     /gene="tag"
                     /locus_tag="ACHK07_003709"
     CDS             complement(16396..16959)
                     /gene="tag"
                     /locus_tag="ACHK07_003709"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO8103991.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            17117..17815
                     /gene="yhjY"
                     /locus_tag="ACHK07_003710"
     CDS             17117..17815
                     /gene="yhjY"
                     /locus_tag="ACHK07_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8103992.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGSWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            18044..19246
                     /gene="yhjX"
                     /locus_tag="ACHK07_003711"
     CDS             18044..19246
                     /gene="yhjX"
                     /locus_tag="ACHK07_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8103993.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLIISLALSTTIRQPQQ
                     KVLREAHI"
BASE COUNT         4737 a         4621 c         5009 g         4957 t
ORIGIN      
        1 ctttaatgcc ttcttgtttg gcttctttta caacgttact taatacctga ccgttatacg
       61 gaataatgac aagaacatcg acaccccggt ttatcatgtt ttcaatctgc gacatttgtg
      121 tttcttcatt gccatttgca gactgtacaa atactttcgc gccgagagat tctgcctttt
      181 tcacaaagat atctcgatct ttttgccagc gttcaagacg gagatcatca atcgccatac
      241 ctattttgac ttctttggcg tgtgcagcaa cgttggtaag caggagtgag gtgcaaaggg
      301 tgagtagaat gttctttatt ttcatggtgt agggccttct gtagttagag gacagcttta
      361 ataagtaaca atcaccgcga taaacgtaac caatttttag caactaaaca ggggaaaaca
      421 attacagatt tttatctttc gattacgatt tttggtttat ttcttgattt atgaccgaga
      481 tcttactttt gttgcgcaat tgtacttatt gcatttttct cttcgaggaa ttacccagtt
      541 tcatcattcc attttatttt gcgagcgagc gcacacttgt gaattatctc aatagcagtg
      601 tgaaataaca taattgagca actgaaaggg agtgcccaat attacgacat catccatcac
      661 ccgcggcatt acctgattat ggagttcaat atgcaagcct attttgacca gctcgatcgc
      721 gttcgttatg aaggctcaaa atcctcaaac ccgttagcat tccgtcacta caatcccgac
      781 gaactggtgt tgggcaagcg tatggaagag cacttgcgat ttgccgcctg ctactggcac
      841 accttctgct ggaacggggc ggatatgttt ggtgtggggg cgtttaatcg tccgtggcag
      901 cagcctggtg aggcactggc gttggcgaag cgtaaagcag atgtcgcatt tgagtttttc
      961 cacaagttac atgtgccatt ttattgcttc cacgatgtgg atgtttcccc tgagggcgcg
     1021 tcgttaaaag agtacatcaa taattttgcg caaatggttg atgtcctggc aggcaagcaa
     1081 gaagagagcg gcgtgaagct gctgtgggga accgccaact gctttacaaa ccctcgctac
     1141 ggcgcgggtg cggcgacgaa cccagatcca gaagtcttca gctgggcggc aacgcaagtt
     1201 gttacagcga tggaagcaac ccataaattg ggcggtgaaa actatgtcct gtggggcggt
     1261 cgtgaaggtt acgaaacgct gttaaatacc gatttgcgtc aggagcgtga acaactgggc
     1321 cgctttatgc aaatggtggt tgagcataaa cataaaatcg gcttccaggg cacgttgctt
     1381 atcgaaccga aaccgcaaga accgactaaa catcaatatg attacgatgc tgcgacagtc
     1441 tatggcttcc tgaaacagtt tggtctggaa aaagagatta aactgaacat tgaagctaac
     1501 cacgcgacgc tggcaggtca ctctttccat catgaaattg ccaccgccat tgcgcttggc
     1561 ctgtttggtt ctgtcgacgc gaaccgtggc gatgctcaac tgggctggga caccgatcag
     1621 ttcccgaaca gtgtggaaga gaatgcgctg gtaatgtatg aaattctcaa agcaggcggt
     1681 ttcaccaccg gcggtctgaa cttcgatgcc aaagtacgtc gtcaaagtac tgataaatat
     1741 gatctgtttt acggtcatat cggcgcgatg gatacgatgg cactggcgct gaaaattgca
     1801 gcgcgcatga ttgaagatgg cgagctggat aaacgcatcg cgcagcgtta ttccggctgg
     1861 aatagcgaat tgggccagca aatcctgaaa ggccaaatgt cactggcaga tttagccaaa
     1921 tatgctcagg aacataattt gtctccggtg catcagagtg gtcgccagga gcaactggaa
     1981 aatctggtaa atcattatct gttcgacaaa taacggctaa ctgtgcagtc cgttggcccg
     2041 gttatcggta gcgataccgg gcatcttttt aaggaacgat cgatatgtat atcgggatag
     2101 atcttggcac ctcgggcgta aaagttattt tgcttaacga gcagggtgag gtggttgctt
     2161 cgcaaacgga aaagctgacc gtttcgcgcc cgcatccact ctggtcggaa caagacccgg
     2221 aacagtggtg gcaggcaact gatcgcgcaa tgaaagctct gggcgatcag cattctctgc
     2281 aggacgttaa agcattgggt attgccggcc agatgcatgg agcaacctta ctggatgctc
     2341 aacaacgggt attgcgccct gccattttgt ggaacgacgg gcgctgtgcg caagagtgca
     2401 ctttgctgga agcgagagtt ccgcaatcac gagtgattac cggcaacctg atgatgcccg
     2461 gatttactgc gcctaaattg ctatgggttc agcggcatga gccggagata ttccgtcaaa
     2521 tcgacaaagt attattaccg aaagattact tgcgtctgcg tatgacggga gagtttgcca
     2581 gcgatatgtc tgacgcagct ggcaccatgt ggctggatgt cgcaaagcgt gactggagtg
     2641 acgtcatgct gcaggcttgc gacttatctc gtgaccagat gcccgcatta tacgaaggca
     2701 gcgaaattac tggtgctttg ttacctgaag ttgcgaaagc gtggggtatg gcggcggtgc
     2761 cagttatcgc aggcggtggc gacaatgcag ctggtgcggt tggtgtggga atggttgatg
     2821 ctaatcaggc aatgttatcg ctggggacgt cgggggtcta ttttgctgtc agcgaagggt
     2881 tcttaagcaa gccagaaagc gccgtacaca gcttttgcca tgcgctaccg cagcgttggc
     2941 atttaatgtc tgtaatgctg agtgcagcgt cgtgtctgga ttgggccgca aaattaaccg
     3001 gcctgagcaa tgtcccagct ttaatcgctg cagctcaaca ggctgatgaa agtgccgagc
     3061 cagtttggtt tctgccttat ctttccggcg agcgtacacc acacaataat ccccaggcga
     3121 aaggggtttt ctttggtttg actcatcaac atggccccaa tgaactggcg cgagcagtgc
     3181 tggaaggcgt gggttatgcg ctggcagatg gcatggatgt cgtgcatgcc tgcggcatta
     3241 aaccgcaaag tgttacgttg attggtggcg gggcgcgtag tgagtactgg cgtcagatgc
     3301 tggcggatat cagcggtcag cagctcgatt accgtacggg gggggatgtg gggccagcac
     3361 tgggcgcagc aaggctggcg cagattgcgg cgaatccaga gaaatcgctc attgagttgt
     3421 tgccgcaact accgttagaa cagtcgcatc taccagatgc gcagcgttat gccgcttatc
     3481 agccacgacg tgaaacattc cgtcgcctct atcagcaact tctgccatta atggcgtaaa
     3541 cgttatcccc tgcctgaccg ggtgggggat aattcacatc tatatatctc agcaattaat
     3601 taatatttag tatgaattta ttctgaaaat catttgttaa tggcattttt cagttttgtc
     3661 tttcgttggt tactcgtaat gtatcgctgg tagatatgga gatcgttatg aaaacctcaa
     3721 aaactgtggc aaaactatta tttgttgtcg gggcgctggt ttatctggtt gggctatgga
     3781 tctcatgccc attgttgagt ggaaaaggct atttttttgg cgtgttaatg acagcaactt
     3841 ttggcaacta tgcatatctt cgcgcagaaa aactcgggca actggataat ttttttaccc
     3901 atatttgcca gttagttgcg ttaatcacta tcggtctctt gtttatcggt gttttaaatg
     3961 cacctatcaa tgcttatgaa atggtgatct accccatcgc cttttttgtc tgcttgtttg
     4021 gtcaaatgcg tttgtttcgc tcggtatgag caacataaag ctcttacaca ttcaggaatg
     4081 aaaggaatac tgtgatggac aacaaaatat caacctattc actggccttt agtattgtgt
     4141 catggatagc tctcgttggt ggtatcgtta cctatctgtt agggctatgg aatgcagaga
     4201 tgcagttaaa tgaaaaagga tattattttg ccgtactggt attaggactg ttttctgcgg
     4261 cgtcttatca aaagaccgtt cgggacaagt atgaaggcat accgaccact tccatttatt
     4321 atatgacctg cctgactgtc tttattatct ctgttgcatt actgatggta ggtctgtgga
     4381 atgcgacatt attactcagc gaaaaaggtt tttatggact ggctttcttc ttaagcttgt
     4441 ttggtgcagt agcggtgcag aaaaatattc gtgatgccgg aataaaccca ccaaaagaaa
     4501 cacaggttac ccaggaagaa tacagcgaat aactcacgta agcccggtca gtccaatgtg
     4561 accgggcttt tacttaactc actaatctgt ttctgtcgat tcgttgtacc agcatagaaa
     4621 gtaacaaact cgctgccaac gtcgcgcaaa aaatccaaat aatatccagt attggccaat
     4681 ttttaagctc aattccccgg gtgcgcagtg catggataat caaggcgtgg aatccgtata
     4741 tacccaacga atggcgggag attaagccaa gtccgcgaat ggtacgcgta tccagcgtgt
     4801 ttttaaccag agtcaatagc gcgattgcgc agataaaaac catcggccca cagtaaagat
     4861 accaggtatc ggcaaaattt ccgcgccact gcaattcata taatgtcccg cgagagataa
     4921 taaaaactcc cgtcgcaaac agtgcggcgc tcacccacga cagtgcttta tgctgtgtgt
     4981 ccatcatccc tatagcgcgg cccaacatgc catacagaat gtagtaaaaa gtatcgccat
     5041 tgatatataa gttaattggc agccattcaa aaccgtcgat tttctgcggc acggtgtttg
     5101 ggttagcgat gataccaatc accaccatta gcaccagcaa catttttccg ccgacgttct
     5161 tcacctgaat cagcggtgaa accagataaa tcaccgcaat cgcgaagaaa aaccataagt
     5221 ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa ctccacatta atggaggtaa
     5281 acagtgcaat gtagagcagc gcgattgcgc tataaaaaaa cagacataag ccgatacgca
     5341 agaaatggcg cggctgggcg ctgcgttcgc caaaaaagag atagccggaa atcatgaaaa
     5401 atagcggcac gctgacacga gaggcagagt tcagaacatt ggcgatatcc caggtgacgg
     5461 ggctaacact atgagcattg gtcacatacc aggtagtggt gtgaatcatc accaccatta
     5521 aacacgctat ccctcgcagg ttatcaatcc agtaaatttt gggctgcatc tgtgtctctg
     5581 tatctggtta aaaaaagtct gaccgataaa tcattcagtt ggcgcactgg aataatctga
     5641 gttttaacac tacagcttat agaggcttaa ggaaattcgt aagatatcag ccactatacc
     5701 gatataaata ataagactca cctgcaaacc agacggtaat ttaatgatga tgaacgcttt
     5761 ctttccggca atggcgctta tggtgctagt gggttgttct acaccgtcac ccgtgcagaa
     5821 agcacaacgg gtaaaggttg atcctctgcg ttcgttgaat atggaagcgt tatgcaagga
     5881 tcaggcggca aaacgttata acaccggcga gcaaaaaatc gacgtcaccg ccttcgaaca
     5941 gttccaggga agctatgaaa tgcgcggtta taccttccgt aaagagcagt ttgtctgttc
     6001 ttttgacgca gatggccatt ttttgcatct ttccatgcgt taagtcctgc tttttcccgt
     6061 ttcatactgt atatcttcca tccagcgggt atactgatcc cttcctttaa atccacacgt
     6121 atccagcacg aaataatatg caaaagtttg ataccaggac cttccagggc ttgatcctga
     6181 ccttacagga ttactgggct cgccagggct gcaccattgt tcaaccattg gacatggaag
     6241 tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc gctggggcca gaaccgatgg
     6301 cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg tcgctacggc gaaaacccca
     6361 accgtttaca gcactactat cagttccagg tggtcattaa gccatcgccg gacaatattc
     6421 aggagctgta ccttggttct ctgaaagagc tgggcatgga cccgactatc cacgatatcc
     6481 gtttcgtgga agataactgg gaaaacccga cgctgggtgc ctggggactg ggctgggaag
     6541 tgtggctaaa cggcatggaa gtgacgcagt tcacttactt ccagcaggtt ggtggtctgg
     6601 agtgtaaacc ggttaccggc gagatcacct acggtctgga acgtctggcc atgtacattc
     6661 agggcgtaga cagcgtttac gacctggtct ggagcgacgg cccgctgggt aaaaccacct
     6721 acggcgacgt gttccatcag aacgaagtgg agcagtccac ttacaacttc gaatacgcgg
     6781 acgtggactt cctgttcacc tgctttgagc agtacgagaa agaagcgcag cagctgctgg
     6841 cgctggaaaa tccgctgccg ctgccagctt acgagcgtat tctgaaagcc gcccacagct
     6901 tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga gcgtcagcgc tacattctgc
     6961 gcattcgcac cctgaccaaa gcagtggcag aagcatacta cgcttcccgt gaagccctcg
     7021 gcttcccgat gtgcaacaaa gataagtaag aggcggctat gtctgagaaa acttttctgg
     7081 tggaaatcgg cactgaagag ctgccaccaa aagcactgcg cagcctggct gagtcctttg
     7141 ctgcgaactt tactgcggag ctggataacg ctggcctcgc acacggcacc gttcaatggt
     7201 ttgctgctcc gcgtcgtctg gcgctgaaag tagctaacct ggcggaagcg caaccggatc
     7261 gtgaaatcga aaaacgcggc ccggcgattg cccaggcgtt cgacgctgaa ggcaaaccga
     7321 gcaaagcggc agaaggttgg gcgcgtggtt gcggtattac cgttgaccag gctgagcgtc
     7381 tgactaccga taaaggtgaa tggctgctgt atcgcgccca cgtgaagggc gaaagcactg
     7441 aagcactgct gccgaatatg gttgcgactt cgctggcgaa actgccgatt ccgaaactga
     7501 tgcgctgggg tgcaagcgac gtgcacttcg tgcgtccggt gcacaccgtg accctgctgc
     7561 tgggcgacaa agtcattccg gcaaccattc tgggcattca gtccgatcgc gtgattcgcg
     7621 gccaccgctt tatgggcgag ccggaattca ccatcgataa cgccgatcag tatccggaaa
     7681 ttctgcgtga gcgcgggaaa gtcatcgccg attacgaaga acgtaaggcg aagattaaag
     7741 ccgatgccga agaagcagcg cgtaagattg gcggtaacgc tgacttaagc gaaagcctgc
     7801 tggaagaagt ggcttcgctg gtggagtggc cggtcgttct aaccgcgaaa tttgaagaga
     7861 aattcctcgc ggtgccgtct gaagcactgg tttacaccat gaaaggtgac cagaagtact
     7921 tcccggtgta tgcgaacgac ggcaaactgc tgccgaactt tatcttcgtt gccaacatcg
     7981 aatcgaaaga tccgcagcaa attatctctg gtaacgagaa agtcgttcgt ccacgtctgg
     8041 ctgatgccga gttcttcttc aacaccgacc gtaaaaaacg tctggaagat aacctgccgc
     8101 gcctgcaaac cgtgctgttc cagcaacagc tgggtacgct gcgtgacaag actgaccgca
     8161 tccaggcgct ggctggctgg atcgccgaac agattggcgc tgacgttaac cacgcaaccc
     8221 gtgcgggcct gctgtctaag tgtgacctga tgaccaacat ggtcttcgag ttcaccgaca
     8281 cccagggcgt tatggggatg cactacgcgc gtcacgatgg cgaagcggaa gacgtagccg
     8341 tcgcgctgaa tgagcagtat cagccgcgct ttgccggtga tgacctgccg tccaacccgg
     8401 tagcttgtgc gctggcgatt gctgataaga tggatactct ggcgggtatc ttcggtatcg
     8461 gtcagcatcc gaaaggcgac aaagacccgt ttgcgctgcg tcgtgccgca cttggcgtgc
     8521 tgcgaattat cgttgagaag aacctcaacc ttgatctgca aacgctgacc gaagaagcgg
     8581 tgcgtctgta tggcgataag ctgactaatg ccaacgtagt tgatgatgtt atcgacttta
     8641 tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta taccgttgac accatccagg
     8701 cggtactggc gcgtcgtccg actcgtccgg ctgatttcga tgcccgcatg aaagcggtat
     8761 cgcacttccg taccctggaa gcagctgcag cactggcggc ggcgaacaaa cgtgtatcta
     8821 acatcctggc gaaatctgac gaagtgctga gcgatcgcgt gaatgcctct actctgaaag
     8881 agccggaaga aattaaactg gcgatgcagg ttgtggtgct acgtgacaag ctggagccgt
     8941 actttgcgga aggtcgttac caggatgcgc tggtcgaact ggcagagctg cgtgaaccgg
     9001 tcgatgcctt cttcgataaa gtgatggtca tggttgatga caaagaattg cgtatcaacc
     9061 gtctgaccat gctggagaaa ctgcgcgaac tgttcctgcg cgttgcggat atttcgctgt
     9121 tgcagtaata acgccgttat taaaaagcct gccatctggc aggctttttt tatcgctaaa
     9181 taatacagca acctttaata atcttctgct gaataaagat tatctcatat attaatttta
     9241 tgagattttt taggattata tcaaggagaa gaaacaaact tattaagcta gaatagccac
     9301 gggtgcttga gactgtttgt ctcaggtatt caccgaaagg cagacagaga aaagccccac
     9361 ctgactataa atcaaagtga ggcccctcta tgcatagaca ccataaggtt agcctcttac
     9421 tcgttggaaa tcaacacagg gggctgggaa tgccgcagaa atatagattg ctttctttaa
     9481 tagtgatttg tttcacgctt ttatttttca cctggatgat aagagattca ctgtgtgaat
     9541 tgcatattaa acaggggagt tatgagctgg cggcattttt agcctgcaat ttgaaagagt
     9601 aagagtcttc ggcgggaagt tattcccgcc tttcttacgg cgttgcgcat tctcattgca
     9661 cccaaattta tttttcacaa aaataatact agattttatt acgcgatcga ttatttattt
     9721 cctgaaaaac aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt
     9781 aagcagagat tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat
     9841 ggtgaaggac actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga
     9901 gaagtgtacg aacacgtctt tagagccatc gtcaggagtg atgaagccga agcctttgtc
     9961 agcgttgaac cattttacga taccagtcat tttaccggac atagtgtatt acctttaata
    10021 attaagtgtg cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg
    10081 tgcactacga ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta
    10141 atggtctgta cgtcaaaccg ttgatgtgca ttaagccacg cattggcggg tgatgcaaca
    10201 attatttttc atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa
    10261 ccgcaataaa gtggataaaa gtctattcca tcaactgctt acttaatgcc gggttggctt
    10321 gaatcaaacg catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt
    10381 cctttaccat ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg
    10441 tcccttttcg taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt
    10501 tctgcgtttc atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat
    10561 ctttatattc cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc
    10621 tcattaagaa tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct
    10681 atgattcgat tcagccagtc atttagcgtg acttatagcc acctaacgat ctgataaatg
    10741 ccgatatatc tcttaccact cctggtttgt aaagatttcg aaaatctaac cttctgcgaa
    10801 ggcgtaaatc tctggctaca aaaaaggcaa cggcaatatc attttttaac aatgaaacaa
    10861 caaattaacc ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta
    10921 tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc
    10981 tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg
    11041 cgcctcttgc ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg
    11101 atcagcgtaa agcgtttatc gacttcctgc agaatacagt gatgcgtagc ggtgaacgtc
    11161 taccaaccct gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga
    11221 ttctgtatgg ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggttg
    11281 tagacagcgt taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc
    11341 gtgaaatgaa tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg
    11401 ccgatgctgc gcactctgcg ctaaaacaga gtgatgacct gaaaccggtc tttgatcagg
    11461 cattcaccaa agtggtaacg acgccagctg atgcattgca accattgatt ccggcggcac
    11521 aaactttcac gcaacaactg gtgatggttg gagactacat tgcccaacaa ggtactcagg
    11581 tgagctttgt agcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca
    11641 aactgattgc gccattaccg gcacagcatc aggcgtttaa tcaggcctgg actacagcag
    11701 ttactgcaac ccaataaaga gtaaaagccc gagtgattat cgggctttac tccacgcagt
    11761 cgcggcttag tccgcgacgt gcggattcac acagttcttc tcaacctttc ctagtaacgc
    11821 atcaatcaaa ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga
    11881 tccaatatgc ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggaaag
    11941 tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgcagc
    12001 aatcagtgca ttttcatcaa ccaccgggcc acgtccggca ttaatgaata tggcggagga
    12061 tttcattttg gcgaattgtt ctgcgccaaa tagatgatac gtctcatcag ttaacggcag
    12121 gatcaggcaa acgaaatctg actcttgtaa cagtgtatcc aaatcgcagt agcgggcgtt
    12181 gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa
    12241 gccaaagtgc gcacgttgtg ccagcgccat accgatccgt cccatcccga caatgcccag
    12301 tgttttatgg tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc
    12361 tgcttttacc cgttctgcta cctccacaac ccgacgagcg gtagacaaca tcagcgccat
    12421 cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg tgcatgagca gaatttttcg
    12481 ggcggtaagc gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg
    12541 cagtttcggc attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc
    12601 ttcagcttcg gcaaaaattg ctgcattttg atcgacggtt tgtgggctga ggtttgccac
    12661 ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta
    12721 gaggataacg gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct
    12781 gaaatatgat gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa
    12841 gggattacag cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg
    12901 tgctgttgct ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt
    12961 ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc acgctgttgg gagagacgca
    13021 tgttcagatc gtgaccaccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg
    13081 gatactcttt cagcaccatt gccacgccgg tcagggtgtt agcgcccgca ggtttcaggg
    13141 tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc
    13201 tgcgggttac actaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat
    13261 ccatgtaata acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc
    13321 ctttaccgcg atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc
    13381 ccagacctgc gccgatagca gatttacctg cttcgcgttc gccggtgtaa gggttagttg
    13441 tgcagccaga taccgccaga gcgccactca ctacggcggc aataagataa acacgtttct
    13501 tcattgttaa tccttaataa cctttttatt ctttgccacg ggttccgtgg cgggagatta
    13561 tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa
    13621 ctgctcaata catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat
    13681 tccgttctga ctgccgccca ttgggggccc atgctggttg aaaccgacgg cgaaaccgtg
    13741 tttagctcgc gtggcgcgtt agccacagaa atggaaaact ccttgcagag cgcggttcgc
    13801 gaccaggttc acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg
    13861 tcaccggaaa acccgcaagg cattcgtggg caggatgaat ttgttcgcgt gagttgggat
    13921 gaggcgctgg atcttattca ccaacaacat aaacgcattc gtgaggctta tggtccggca
    13981 tcgatttttg ctggttccta cggctggcgt tcaaacggcg tgctgcataa ggcctcgaca
    14041 ttattacaac gctatatggc gctggcaggc ggttataccg ggcatctggg ggattattcg
    14101 accggcgcgg cacaggcgat catgccgtat gtcgtgggtg gtagtgaagt ttatcaacag
    14161 cagaccagtt ggccgctggt gctggaacat agcgatgtcg tggtgctgtg gagtgctaac
    14221 ccactcaata cgctgaaaat tgcgtggaat gcatccgatg agcaggggct ttcttacttt
    14281 tctgcactgc gtgacagcgg gaaaaagctg atctgcattg atccaatgcg atcggaaacc
    14341 gtcgatttct ttggcgataa aatggagtgg gtggcaccgc acatgggcac tgatgttgcg
    14401 ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt ggcacgacga agcgtttctg
    14461 gcgcgttgca ccacaggtta tgccgtcttc gcctcttatt tgctgggcga gagtgacgga
    14521 atagcgaaaa ccgccgaatg ggcagcagag atttgtggtg ttggcgcagc gaaaatccgc
    14581 gagctggcgg ctattttcca ccaaaatacc accatgctga tggctggctg gggaatgcag
    14641 cgccaacagt ttggtgagca aaaacactgg atgatcgtca cgctggcagc aatgttgggg
    14701 caaatcggca cacccggcgg cggttttggt ctttcttacc attttgccaa tggtggtaac
    14761 cccacgcggc gcgctgcggt actctcttcc atgcagggca gcttgccggg tggctgcgat
    14821 gcggtagata aaatccctgt cgcccgcatt gttgaagcac tggaaaaccc cggtggcgca
    14881 tatcaacaca acggtatgga ccgacatttc ccggatattc gttttatctg gtgggcgggc
    14941 ggtgccaact ttactcatca tcaggatacc aaccgcctga tccgtgcctg gcaaaaaccg
    15001 gagctggtgg tgatctccga atgtttctgg acggcggcgg cgaaacacgc ggatatcgtt
    15061 ctgcctgcga ctacctcttt tgagcgtaat gatctcacca tgaccggtga ttacagtaat
    15121 cagcatctgg tgccgatgaa gcaagtggtg ccgccacgct atgaagcgcg taatgatttt
    15181 gatgtttttg ccgagttaag tgaacgctgg gagaagggcg gctatgcgcg gtttacggaa
    15241 ggaaaaagtg agctgcaatg gctggaaatg ttttataacg ttgcccgaca gcgcggggca
    15301 agccagcagg ttgaattgcc gccatttgct gagttctggc aagccaatca gttaattgag
    15361 atgccggaaa acccggacag cgagcggttt attcgcttcg ctgatttttg ccgcgatccg
    15421 caggcgcatc cattaaaaac cgccagcggt aagattgaaa tcttctcgca gcgtattgcc
    15481 gattacgctt atccggattg cccggggcat ccaatgtggc tggagccgga cgaatggcag
    15541 ggtaatgccg aaccggaaca gttacaggta ctttctgctc atccggcgca tcgtctgcac
    15601 agccagctga attacagttc tctgcgcgaa ttgtacgcgg tagcaaaccg tgagcctgtg
    15661 accattcatc ctgacgatgc tcaggcgcgc ggcatacaag atggcgatac tgttcggctg
    15721 tggaactcac gcgggcaaat tcttgccgga gcggtcatta gcgagggaat taaacctggc
    15781 gtgatttgca ttcacgaagg ggcatggccg gatctggatt taaccgctga cggtatttgt
    15841 aaaaacggcg cagtgaacgt gctgaccaaa gatctcccca gctcgcggct ggggaatggc
    15901 tgtgcgggta atacggcgct ggcatggcta gaaaaataca acggtccgga actgacactt
    15961 acagcgtttg aaccaccggc cagctcataa tccaggtggg tagttgggtt tcatcctgcc
    16021 atgcgcaatc gacaatgtga aaaccctgcg cctggtaaaa atctatcgcc ggttgatttt
    16081 tttgataaac ctccagcatc agatggggat ggcgctgttg cacatactgc atcagcgcct
    16141 taccaatacc gcgcctgacg gccttcggtg cgacaaacat cgctgccaga aatcggcctt
    16201 ccataatgct gacaaaacca agaagcttac tgtcttcttc ccagacccag ttttgtgcgt
    16261 tggcaagata ggcatcccgc accagcggaa tgcactcgcg ccagtaattc gcttttataa
    16321 agggatggcc ccaggttgta ctttccagcc acagttcgag gatcgcgggg agttctgaac
    16381 gttgcgcttc cctaatcatg gtttatttcc cggatagcaa cagcagccaa ccacatgatc
    16441 attcaccagc ccacatgcct gcataaagga gtaacagatt gtggtaccga caaacttaaa
    16501 accacgtttt ttcagtgcct tagatagggc gtcggaggcg ggcgtagatg tggggatttc
    16561 gctcaacgtt gtggcttgtg tcacctgtgg ctgatgattt acaaacgacc agacaaagtc
    16621 ggcaaacggt tcgccgttct gttccatttg caggtacgcc cgcgcattac caataattgc
    16681 ctgaattttc ccgcgatggc ggataatccc ggcgtcctgt accagtcttt cgacatcctc
    16741 ttcctgcatt gcggcaacct tcaccggatc gaactgatga aagcaggcgc gatagttttc
    16801 gcgttttttg aggacggtga tccacgataa cccagcttgc tgcccttcca ggcagatcat
    16861 ttcgaacagt tttttactgt cagtttcagg cacgccccac tcattatcat ggtaggcaat
    16921 ataaagcggg tcctgactca cccagccgca acgttccata ctttccctcg ctatgtaccc
    16981 gatcgtaaaa atttcagccg cttttcttga cgcatcggtg aaaaagtcaa tatttaatac
    17041 agggctacta tgcccgatat ccttcataca atgacaataa tattgccgaa ttcggctctt
    17101 ccctggagtc gtgttaatga tcataaaaaa aagcggtggt cgctggcagc taagcctgct
    17161 ggcgagtgtg gtaatcagtg ccttttttct caacacagct tacgcctggc aacaagaata
    17221 tatcgttgat acgcaacccg ggcattccac agagcgttac acctgggata gtgatcatca
    17281 acctgattac aacgatattt tgtcgcaacg tattcaaagt agccaaaggg cgctgggact
    17341 ggaagtcaat ctggcggaag aaacccctgt ggatgtgacc agcagtatga gtatgggctg
    17401 gaattttcct ttatatgaac aggttacaac tggcccggtc gcggcattac attacgatgg
    17461 cacaaccacc tcgatgtata acgagtttgg cgacagtact accacgctga ccgatccgtt
    17521 atggcatgcc agcgtgagta ccttaggctg gcgtgttgac tcccggcttg gcgatctccg
    17581 accctgggcg caaatcagct ataaccagca atttggcgag aatatctgga aggcgcaatc
    17641 aggcctgagc cggatgacgg cgacaaacca gaacggcagc tggctggatg tcaccgtagg
    17701 cgctgatatg ttgctcaatc aaaatattgc cgcctatgcc gcgctatctc aggcagaaaa
    17761 taccactaac aatagcgatt atctgtatac gatgggggtt agtgccagat tttaacgtaa
    17821 caatcacaat tgaaaccatt gaataacaat agttgtggcg atagtgggtg ctaacttacc
    17881 aaataataaa tttggtgaat aattgtcgcg tcattcattc ctgaactaag gcatttcatt
    17941 ccgttctgat ggcatttcat gccgtttttc cccaggcata aagtgcactt cgttatggtt
    18001 gtcggcagag atttttcctt tttattactg caggaatact gccatgacac cttcaaatta
    18061 tcagcgtacc cgctggctga cactcatcgg tactatcatt acccagtttg cgctggggtc
    18121 ggtttatacc tggagcctgt ttaacggcgc gctttccgcc aagctggatg cgccggtaag
    18181 ccaggtcgct ttctctttcg gcttgttaag tctggggctg gcaatttcgt cttctgttgc
    18241 gggcaaattg caggaacgtt ttggtgttaa acgcgtcacc atcgcttccg gcattttgct
    18301 gggattaggg ttcttcctga cagcgcattc tgacaacctg atgatgctgt ggttaagcgc
    18361 cggtgtgctg gtcggtctgg cggatggcgc gggttacctg ctgacgctct ctaactgcgt
    18421 gaaatggttc ccggagcgta aaggtctgat ctccgcgttc gctatcggtt cttatggtct
    18481 gggtagcctg ggtttcaaat ttatcgacac gcagctgctg gaaacggtcg gtctggaaaa
    18541 aacctttgtg atttggggag cgattgcgct ggtgatgatt gttttcggcg caacgttaat
    18601 gaaagacgca ccaaaacagg aagtgaaaac cagcaatggt gtggtggaga aagattacac
    18661 gctggcagag tcgatgcgta aaccgcagta ctggatgtta gcggtaatgt tcctgactgc
    18721 ctgcatgagc ggcctgtacg tgattggggt agcgaaagat atcgcccaaa gtctggcaca
    18781 ccttgatgtg gtttctgcag ccaatgcagt cactgttatt tccatcgcca acctttcagg
    18841 tcgtctggtg ctgggcattc tgtctgacaa aatcgcccgt atccgtgtta tcaccattgg
    18901 tcaggtgatt tcgctggtgg gtatggcggc cctgctgttt gcaccattga atgcagtgac
    18961 gttctttgca gcgattgcct gtgtggcatt taactttggc ggcactatta ccgtctttcc
    19021 gtcactggtc agtgagttct tcggcctcaa taacctggcg aaaaactacg gtgtgattta
    19081 cctcggtttc ggtatcggta gcatttgtgg ttcgattatc gcctcactgt ttggcggctt
    19141 ctatgtgacg ttctacgtca tctttgctct gctgattatt tccctggcac tttccaccac
    19201 cattcgccag ccgcagcaga aagtgttgcg tgaggcgcat atttaatgag atggcgttgg
    19261 ttattaacga aggggcgaga gagccccttt ttttcgttta aaataataag gagattacaa
    19321 aact
//
LOCUS       DAZGCQ010000093        19171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000093 DAZGCQ010000000
VERSION     DAZGCQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.093"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..399)
                     /gene="uspD"
                     /locus_tag="ACHK07_003712"
     CDS             complement(<1..399)
                     /gene="uspD"
                     /locus_tag="ACHK07_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO8103994.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSAD"
     gene            complement(426..725)
                     /gene="yiiS"
                     /locus_tag="ACHK07_003713"
     CDS             complement(426..725)
                     /gene="yiiS"
                     /locus_tag="ACHK07_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO8103995.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(937..1377)
                     /gene="yiiR"
                     /locus_tag="ACHK07_003714"
     CDS             complement(937..1377)
                     /gene="yiiR"
                     /locus_tag="ACHK07_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8103996.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            1478..2077
                     /gene="yiiQ"
                     /locus_tag="ACHK07_003715"
     CDS             1478..2077
                     /gene="yiiQ"
                     /locus_tag="ACHK07_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO8103997.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            2185..2952
                     /gene="tpiA"
                     /locus_tag="ACHK07_003716"
     CDS             2185..2952
                     /gene="tpiA"
                     /locus_tag="ACHK07_003716"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO8103998.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(3007..3762)
                     /gene="cdh"
                     /locus_tag="ACHK07_003717"
     CDS             complement(3007..3762)
                     /gene="cdh"
                     /locus_tag="ACHK07_003717"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO8103999.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESVTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(3869..4858)
                     /gene="sbp"
                     /locus_tag="ACHK07_003718"
     CDS             complement(3869..4858)
                     /gene="sbp"
                     /locus_tag="ACHK07_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO8104000.1"
                     /translation="MNKWGVGLTFLLATTSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(5177..6139)
                     /gene="pfkA"
                     /locus_tag="ACHK07_003719"
     CDS             complement(5177..6139)
                     /gene="pfkA"
                     /locus_tag="ACHK07_003719"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO8104001.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(6320..7222)
                     /gene="fieF"
                     /locus_tag="ACHK07_003720"
     CDS             complement(6320..7222)
                     /gene="fieF"
                     /locus_tag="ACHK07_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO8104002.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(7371..7871)
                     /gene="cpxP"
                     /locus_tag="ACHK07_003721"
     CDS             complement(7371..7871)
                     /gene="cpxP"
                     /locus_tag="ACHK07_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO8104003.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8021..8719
                     /gene="cpxR"
                     /locus_tag="ACHK07_003722"
     CDS             8021..8719
                     /gene="cpxR"
                     /locus_tag="ACHK07_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO8104004.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8716..10089
                     /gene="cpxA"
                     /locus_tag="ACHK07_003723"
     CDS             8716..10089
                     /gene="cpxA"
                     /locus_tag="ACHK07_003723"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO8104005.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10195..10869)
                     /gene="yiiM"
                     /locus_tag="ACHK07_003724"
     CDS             complement(10195..10869)
                     /gene="yiiM"
                     /locus_tag="ACHK07_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO8104006.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENISTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11018..12001)
                     /gene="kdgT"
                     /locus_tag="ACHK07_003725"
     CDS             complement(11018..12001)
                     /gene="kdgT"
                     /locus_tag="ACHK07_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO8104007.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12261..12881)
                     /gene="sodA"
                     /locus_tag="ACHK07_003726"
     CDS             complement(12261..12881)
                     /gene="sodA"
                     /locus_tag="ACHK07_003726"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO8104008.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13166..14200
                     /gene="rhaT"
                     /locus_tag="ACHK07_003727"
     CDS             13166..14200
                     /gene="rhaT"
                     /locus_tag="ACHK07_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO8104009.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(14197..15045)
                     /gene="rhaR"
                     /locus_tag="ACHK07_003728"
     CDS             complement(14197..15045)
                     /gene="rhaR"
                     /locus_tag="ACHK07_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO8104010.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(15119..15955)
                     /gene="rhaS"
                     /locus_tag="ACHK07_003729"
     CDS             complement(15119..15955)
                     /gene="rhaS"
                     /locus_tag="ACHK07_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO8104011.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16243..17712
                     /gene="rhaB"
                     /locus_tag="ACHK07_003730"
     CDS             16243..17712
                     /gene="rhaB"
                     /locus_tag="ACHK07_003730"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO8104012.1"
                     /translation="MTFRNCVAVDLGASSGRVMLTRYERECRSLTLREIHRFNNGLHS
                     QNGYITWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17709..18968
                     /gene="rhaA"
                     /locus_tag="ACHK07_003731"
     CDS             17709..18968
                     /gene="rhaA"
                     /locus_tag="ACHK07_003731"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO8104013.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
BASE COUNT         4755 a         4984 c         4801 g         4631 t
ORIGIN      
        1 atccgcactc aacttattga tcatcccgcg atatgccggc atcaaacggt tgataaatga
       61 gtggtgatga ccacagacaa ggaggtcgca ctgctctttt tgcataattt ccagcagtgt
      121 ttccggcatt tctccgcgtt caatacgcag ttttgtcttc ggccattgaa tatttttcgt
      181 cagtttatac agcttgttat ccgacttatt cttcaacaat tgaagaatat cttctgttgc
      241 agggaagtag atacccgggt acaactcgct taagccatca tcaatatgaa ttaacgtcag
      301 gtgagcgtca ttatgtctgg cgagctccag ggctttattc accagtaagg catcttcttc
      361 attcccggaa attgccacgc caatgtgttt ataagccatg tttaactcct tctaaagcaa
      421 ctccatcaag ctaacgaacg cagtgataac tcaaaaataa tcatctcggc ctggcatgag
      481 aaggtgaagg ccgcatcaag ctcaacttgc ccctccagat ccttaatctt ataggccacg
      541 ctggcccctt catctgcaga ggatgcagcg gcggcaagct ctttcagttt ggtcataaag
      601 gactctgctt cttcgcgggt ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta
      661 tcaatcaccg taccgatatc aatcgcacat cctttagtac tgcatttatc tacgacatct
      721 ttcatagggc acctctgtat attcgcctct ctgtcgatta cgacttaaga ataatgcgcc
      781 gttaagggaa gaacaaacaa gtaatgatct tatggattac catttttttt gatattgctt
      841 aaggtgaaga gttcagaagg attttccggg cattgtcatt tgcatcgtgg aaataacaat
      901 cccggaaaaa aatagcagta agagatatat taaaggttaa ttacttgatt tattgtcggc
      961 tttatacttc acatcctgag tatctttacc atatttattt tcgccttgtg tgccaacaaa
     1021 cgcaccaaga tcgataagca tcatcaccag aatcaacgtc gggacaaaac gccctaccgc
     1081 ccattgccag acacccggta aaatcgccca gttacccgcc agtagcatcc acgccacaat
     1141 catcagaaat gcccatgcgc cggaacgccc gcgatcatgc aagcgcttaa cggttactgc
     1201 cgctgttggc cagagcaagc acacaaggca aaacgccgcg gtctgaatat cgagtaaatt
     1261 tttacccgcc agtgagaaaa gcaccagcat gcctgcgaac cacaggccta tccaaatcca
     1321 gaaatcacgg cgtccaatac gccctttaaa tgagaataac cattgctgta tggtcatgta
     1381 agttccttga tggttgtctt ttccaggatt ctaccctttt gacaagggcg acagatatcg
     1441 ttttaatcgg agccagtcat aacaaaaggt actgtcaatg aagccagggt gtacgctgtt
     1501 ttttctctta tgttctgcat taaccgttac aacaacggcg catgcacaaa caccagatac
     1561 ggcaacgacc gcgccttatc tgctggctgg agcccctact ttcgatctct ccatcagcca
     1621 gtttcgagaa gactttaaca gccagaatcc cagcctgcca ctgaacgaat ttcgtgccat
     1681 cgacagcagt cccgacaaag ccaatctcac tcgtgctgca agtaaaatta acgagaactt
     1741 gtatgcttct acagcgctgg agcgcggtac cttaaaaatc aaaagcattc aaatgacctg
     1801 gctacccatc caggggccag agcaaaaagc cgcgaaagcg aaagctcagg aatatatggc
     1861 agcggtgatc cgcacactca ccccattaat gaccaaaaca caaagccaga aaaaactgca
     1921 gtcgctacta acggcgggga aaaacaaacg ttattacacc gagacagaag gtgcactgcg
     1981 ttatgttgtc gcggacaacg gcgaaaaggg gctgaccttc gctgttgaac cgattaagct
     2041 ggcgctatct gaatcgcttg aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg
     2101 atgaatctct atactgtttc acagacctgc tgccctgcgg ggcggccatc ttcctttatt
     2161 cgcttataag cgtggagaat taaaatgcga catcctttag tgatgggtaa ctggaaactg
     2221 aacggcagcc gccacatggt tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt
     2281 gttgctggct gtgcggttgc aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc
     2341 gaagctgaag gcagccacat catgctgggt gcgcaaaacg tggacctgaa cctgtccggc
     2401 gcattcaccg gtgaaacctc tgctgctatg ctgaaagaca tcggcgcaca gtacatcatc
     2461 atcggccact ctgaacgtcg tacttaccac aaagagtctg acgaactgat cgcgaaaaaa
     2521 ttcgcggtgc tgaaagagca gggtctgact ccggttctgt gcatcggtga aaccgaagct
     2581 gaaaacgaag cgggcaaaac tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa
     2641 actcagggtg ctgcggcatt cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc
     2701 ggtactggca aatctgcaac tccggctcag gcacaggctg ttcacaaatt catccgtgac
     2761 cacatcgcta aagttgacgc taacatcgct gaacaagtga tcattcagta cggcggctct
     2821 gtaaacgcgt ctaacgctgc agaactgttt gctcagccgg atatcgacgg cgcgctggtt
     2881 ggtggtgctt ctctgaaagc tgacgccttc gcagtaatcg ttaaagctgc agaagcggct
     2941 aaacaggctt aagtctgaca ggtgccggat ttcatatccg gcacttactt tccttaactc
     3001 ttcgccttaa cgcaaaatct cacactgatg atcctgaatt tcctcggctg aagcacggtt
     3061 aagcgtcagt agatttcgtt gtgtcgccag caatacaaat gagttatcac tctgccgtac
     3121 catcgccagc ccgtagcttc ccatatgttc ccgcgcctca ggtacttctt ctgccagcat
     3181 cataaatggg ctgcgttgta ccagttcgct ttccgttacc cgacgcgcca ggtattcatg
     3241 cccgcgcaaa ccacctggca gtggcaacca gcggctgctg atgttcgcca gattgttatc
     3301 cagttgttcg cgcacatcag gacgaataca agagatatga atatgaaaat ggttttgcgt
     3361 acgcccggtg cgggagttga tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta
     3421 ttttttgctc ataaaatcac gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg
     3481 atcggtcaac aaaggacttt cagtaccgtt aatacgatac gttggcatca acagatattg
     3541 cagtgggcca ttaagatctt ttaaaaccac gtatccggca ttgggtttga cttccgcaca
     3601 tggcgaagga ttttgatttt gctgctgatt gggcaaacat tcctcaagga caatcttacg
     3661 taatgtaacc gactcttcac cggttaattt ccagtaacca ataccggcag ccacaacggc
     3721 gataactatc atcaccaaaa aaagaagacc cgcttttttc atcttttttt ccctgtacct
     3781 caaagagatg caagggtaac gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc
     3841 accgggtttg ataattatcc tggagaaatc agcgtttgct gatctgatcg aacgtaccgc
     3901 cgttagcaaa atgctctttt tgcgctttcg tccagccgcc gaactcttca tcaatggtga
     3961 ataacttcag ctttggaaac gcattttcgt actttttcgc cacctcagcg tcgcgcggac
     4021 ggtagtagtt tttcgcggca atttcctgac cttctggcga gtagagatat ttcaggtagg
     4081 cttccgccac ctctttagta ccttttttct cgaccacttt atcgaccacc gacacggttg
     4141 gctctgcgag gatagactca ctcggcgtga cgatttcgaa tttatctttc cccagttcat
     4201 tcgctgccag cagagcttcg ttttcccagg caatcagtac atcgccaatt ccgcgctcga
     4261 caaaagtgtt agtggagccg cgcgcgccag aatccagaac ttcgacgttt ttatacagtg
     4321 cccgaacaaa atcctgtgct tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc
     4381 aggcagccag gtaattccag cgcgcgccac cagagctttt cggattaggc gtgatcactg
     4441 aaacacccgg tttaatcaga tcgttccagt catggatctg cttcggattt cccttacgta
     4501 ccaggaaaac aatggtggaa gtgtacggtg cggagttatc cggcagacgt ttgatccact
     4561 ctttatcaat ccgcccgcgt tccgcaattg cgtccacgtc ataggccaga gccagcgtga
     4621 caacatcagc ttcaataccg ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg
     4681 actgacgaat caccacgtta tcaccagttt gctgtttcca gtgggcgctg aatgccttgt
     4741 tgtactgttc gtacaattcg cgcgttggat catatgaaac gttaagaagc tgaatatcct
     4801 ttgccataac gctggttgtc gccagcaaaa atgttaaccc tacgccccac ttgttcatcg
     4861 cccgactctc ttatgttgtg ttgtgatgag caaagcgtgc cagaaggtta accaaacatt
     4921 aaagaataaa aaaagattgg ctataacttg cgggtatatg ttgggggtta aaaaggcgga
     4981 agaaatccgc ctcatattgc tgacaaagtg cgctttatcg atgccggatg cggcgcgaac
     5041 gccttatccg gcctacaaaa gtttgtaaat tcaataaatt gtagaattca cgtaggcctg
     5101 ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg tttcaggcta aaggaatctg
     5161 cctttttccg aaatcattaa tacagttttt tcgcgcagtc cagccagtcg cctttgaacg
     5221 gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg aaccagctgt tcgttctgga
     5281 tacctacgca acgaccgccg taacctgcca gcagcagatc gatagcgtaa gcgcccatac
     5341 gggaagccag aatacggtcg taaggcaccg gagaaccacc gcgctggatg tggcccagca
     5401 cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa atgcgccagt tcgtcaacat
     5461 cacacatatg ttcggtaatc gccacgatcg cgtgtttttt acctttcgcg atacccgctt
     5521 tgatttcgtt taccaggtct tcacggctga attcaacttc cggaaccaca acgaattcac
     5581 agccaccagc aatggccgca gccagcgtca gatcgccaca ataacggccc atcacttcca
     5641 ccacggaaat acgctggtga gaagaagagg tgtcacgcag acggtcgatc gcttctacaa
     5701 cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt gcctttgatg tcgttgtcga
     5761 tagtgccagg cagaccgatg cacgggaagc ccatttcggt cagacgcatt gcacccatgt
     5821 aggaaccgtc accgccgata accaccagcg catcgatccc acgttttttc aggttttcga
     5881 tagccacggc gcggatgttc tcgtcgcgga attccgggaa acgcgcagaa ccgaggaacg
     5941 taccgccacg gttgatcatg tcagaaacgc tgtaacggtc tagctgtacc atacggtctt
     6001 catacagacc cagatagccg tcataaatgc ccattacttc cagaccttct gtcagcgcag
     6061 aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc atcaccgccg cttgtcaaca
     6121 caccgatttt cttaatcatg actacctctg aactttggaa tgcaaaatga aatctgttgc
     6181 cggaagtctt cttacacatc gaagtgatcc aacgaatgtg caaatagtat aacaatcact
     6241 tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt ttatacacaa aatgcgggtc
     6301 tggctctctt ttatactgat tatgaaagca tagaccgttt accctccctg ggtacgacgg
     6361 aacaggggtc ctgatggata atcacatccg atcccggaaa acgccgtaaa atagcctgct
     6421 ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag agagtcttcc atttccaaat
     6481 gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg aagatcgtga gcgccgctaa
     6541 cacccggcca ggaagtcacg atatcaataa tttcttgccg ttcctcatca ggcaatgcgc
     6601 gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa cgcgctatat aaaatataga
     6661 tgccgattcc caatgcaaac agagcatcgg cgcgatgcca gccgtaccag gacaacccca
     6721 gcgcgagcag aattgcgccg ttcatcataa catcagactg gtaatgtagc atatcagccc
     6781 gcaccgcctg gctttgcgtc cggcgcacca cccaacgctg aaacgagaca aggataatcg
     6841 tacaaattag cgccacaatt gtcacgataa ccccgacgcc tggatctgtc atcggtgttg
     6901 gagatatcag atgttgaata cccgtcaaaa acaggaatag tgccgaaccg gagataaaca
     6961 tactttgcgc cagcgccgcg agggactcag ctttaccgtg accaaacgag tgattatcgt
     7021 cggcaggttg cagggaatat cgcaccacca gtaaattcgt caacgacgcg ccgatatcca
     7081 ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc ggtataccac catgcaaaaa
     7141 ttttaatcag caatagcagc gaagccatcg ccgtcgcagc aatcgccgcc cgactgacca
     7201 gccgtccata agattgattc ataaatactc ccgctatcaa ctgacgctag tataacggaa
     7261 gcaaatcatc tgcaatgcat taagcagcag gcaaattgag gataaaaaaa acccccacat
     7321 catgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta ctactgggaa
     7381 cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg cgtcacgtcg
     7441 cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt
     7501 aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc aatttgctca
     7561 ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca
     7621 aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt
     7681 tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc gtcgaacata
     7741 tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc gcctgaaccg
     7801 acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac ggcagcggta
     7861 actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata gagagtttac
     7921 gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat ggattagcga
     7981 cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa tcctgttagt
     8041 tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg aaggcttcaa
     8101 cgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca gcattgattt
     8161 acttttgctt gacgtaatga tgccgaagaa aaatggtatc gacacattaa aagcacttcg
     8221 ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagcgaac ttgatcgcgt
     8281 tctcggcctt gagctgggcg cagatgacta tctcccgaaa ccgtttaatg atcgtgagct
     8341 ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa
     8401 cgacaacggt tcaccgacac tggaagttga tgccttagtg ctgaatccag gccgtcagga
     8461 agccagcttc gacgggcaaa cgctggagtt aaccggtact gagtttaccc tgctctattt
     8521 gctggcacag catctgggtc aggtggtttc ccgtgaacat ttaagccagg aagtgctggg
     8581 caaacgcctg acgcctttcg accgcgctat cgatatgcac atttccaacc tgcgtcgtaa
     8641 actgccggat cgtaaagatg gtcacccgtg gtttaaaacc ttgcgtggtc gcggctacct
     8701 gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat cttctggctg
     8761 acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc acgccagatg
     8821 accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca tgttgaagcg
     8881 gagctggcga acgatccgcc caacgattta atgtggtggc ggcgtctgtt ccgggcgatt
     8941 gataagtggg caccgccagg acagcgtttg ttattagtga ccaccgaagg ccgcgtgatc
     9001 ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc cgataacgcc
     9061 gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt
     9121 gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc tcaatccgat
     9181 ttcattaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat gttggtcagt
     9241 acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac
     9301 gctgccgatg aagttgccca gggaaactta cgccagcacc cggaactgga agcggggcca
     9361 caggaattcc ttgccgcagg tgccagtttt aaccagatgg tcaccgcgct ggagcgtatg
     9421 atgacctccc agcagcgtct gctttctgat atctctcacg agctgcgcac gccactgacg
     9481 cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa ggaactggag
     9541 cgtattgaaa ccgaagcgca acgtctggac agcatgatca acgatctgtt ggtgatgtca
     9601 cgtaatcagc aaaaaaacgc gctggtaagc gagacattga aagctaacca gttgtggggc
     9661 gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac
     9721 ttcccgcctg ggccgtggcc gctgtacggc aatccgaacg ccctggaaag tgcgctggaa
     9781 aacattgttc gtaatgccct gcgttattcc catacgaaga ttgaagtggg ctttgcggta
     9841 gataaagacg gtatcaccat tacggtggac gacgatggtc ctggcgttag cccggaagat
     9901 cgcgaacaga ttttccgtcc gttctatcgg accgatgaag cacgcgatcg tgaatctggc
     9961 ggtacaggtt tggggctggc gattgttgaa accgccattc agcagcatcg tggctgggtg
    10021 aaagcagaag acagcccgct gggcggttta cggctggtga tttggttgcc gctgtataag
    10081 cggagttaaa ctccgcattt gtaggcagga taaggcgttt acgccgcatc cggcatttga
    10141 gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg ggggttattt
    10201 tccccacaac cggcgggaaa aatcttcgat cttgccgctc agtcggcgct tttgcatcgt
    10261 ccgcgtccag cttttcgata acccggcggc ggagagtaaa cggtgatact gatcatcatc
    10321 aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc tgacacggga
    10381 aaccaactcc agcggcgcgt ccgcagacac tttccctggc gctatcacgc tatacaacca
    10441 tcccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa aatggtaatt
    10501 gagtttatag caaggcgagc gcggctggct gacctgaatt aatgcctctc cccagcggaa
    10561 aatatcgcca atataaacat tactttccgt gagaccgtcg gttgagatat tttcgccaaa
    10621 cgcaggcgca acaaacaact ccgcctgttc cggaaattcc cgcgcccagt agagatagtg
    10681 ctcacgagga taatgacaca gcgctctgtc tggcccgccg tgaacctttt tctccgcctg
    10741 ctcgtcacct tccagcccca gctccgtcag catcaactca ccatcaacct ggattttggc
    10801 aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata catcaaccgg
    10861 atatcgcatc tgttccctct ccacgagaaa tttccccatt agaccatatt tattttatgt
    10921 caggcgtgct gttttcgcgg taagtatcca ggcaggaact caatagcaaa ccggacgaac
    10981 gttagttcat ccggctgtgg ggattaagga agcaggttta tttcaccgta cctaaaattt
    11041 cgattttcgc cgctctggct ttgactttac gtgaccagat agaggtaaga attggcacca
    11101 gaatcgaagt cacaatgact gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt
    11161 taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc cctgcggaac
    11221 tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg gcgataatca
    11281 acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc aaacctgtct
    11341 gggcaattac agtcaaatca atggtattgc ccagtgcgaa ggcaaagaat ggaatcagcg
    11401 tctgcaccgc tttgctgaaa aattctcgta attcagggtc aaggttcccc agggcaaagc
    11461 ccaccaggaa cggtaatact gcgccgacga aaacatgcgg ttcaaacgag gcaatccctg
    11521 cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc aacacaaatg
    11581 ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg
    11641 tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa
    11701 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
    11761 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
    11821 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
    11881 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
    11941 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
    12001 tttccatcac ctgtcggcat atttttaagg gctgatgcgt aattcctcaa tggagcatca
    12061 gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca aaaccacatc
    12121 aattgaaaca ctgttttatt tttatcggat cattgttttt attttgaaat tgatcacaaa
    12181 aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcct atacgcctca
    12241 ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc ttcgtcccag
    12301 ttcaccacgt tccagaactc tttgatgtag tccggacggc ggttctggaa tttcaggtag
    12361 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatagct
    12421 tcacccatca gcggagaatc ctggttagca gtagaaacca ccgccagttt atcgcctttc
    12481 agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
    12541 ttgaagttat ctacggaacc gaaatcacgt tcgatagccg ctttcaggtc accctgcagg
    12601 gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc gccagcgttg
    12661 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
    12721 accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt gttgacgtag
    12781 gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
    12841 gcatcgtaag catacggcag ggatggcagg gtatagctca tattcatctc cagtattgtc
    12901 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
    12961 tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg aaaatgcccg
    13021 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac aacggcggca
    13081 acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga gatgtgacgc
    13141 gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga tattttggca
    13201 tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg
    13261 gtcatgggaa accatgtggt cagtcggtgg gattgtttcg tggattattc tgccgtgggc
    13321 catcagcgcc ctgttactac cgaatttctg ggcatattac agctcgttta gtctctctac
    13381 gctgctgcct gtttttctgt tcggcgctat gtgggggatc ggtaatatca actacggcct
    13441 gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca ttacgttgat
    13501 tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgttga ttagcaccga
    13561 aggcggacgc atgacgttgc tcggcgttct ggtggcgctg attggcgtag ggattgtaac
    13621 tcgagccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt tcaatctgaa
    13681 aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt cctttgcgat
    13741 gaacgccgca aaaccgatgc atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt
    13801 cgctctgcca agctatgttg tcatcatggg cggcggcgcg atcattaacc tcggtttctg
    13861 ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa
    13921 accgctgatc attcacaatg tgttactctc ggcactgggc gggttgatgt ggtatctgca
    13981 attctttttc tatgcctggg gccacgcccg cattccggcg cagtatgact acatcagttg
    14041 gatgctgcat atgagtttct atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga
    14101 gtggaacaat gcaggacgcc gtccggtaac ggtgttgagc ctcggttgtg tggtgattat
    14161 tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa tctttctgcg aattgagatg
    14221 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
    14281 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggagaag
    14341 atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca ttccagtctg
    14401 ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
    14461 cagcgcaaag ggacttttca ggctagccgc caaccgggta atcagcttat ccagcaacgt
    14521 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
    14581 caactgcccg aacagcaact ctgccatttc gttagcaaac ggcacatgct gactactttc
    14641 atgctcaagc tgaccgataa cctgccgcgc ctgtgccatc cccatgctac ctaagcgcca
    14701 gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgcc ccctgccagt caagattcag
    14761 cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
    14821 ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc gataagggcg
    14881 atcgttgagt acatgcagac cattaccgcg ccagacaatc accagctcac aaaaatcatg
    14941 tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
    15001 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
    15061 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt
    15121 attgcagaaa gccatcccgt ccctgacgaa tatcacgcgg tgaccagtta aactctcggc
    15181 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccgca atgataggcg atgtcagtaa
    15241 cgctggcctc gctgtgacgt agcagatgtc gagccttcat cagtcgcagg cggttcaggt
    15301 aacgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta cgcagcgaaa
    15361 gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc
    15421 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
    15481 tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc gagggtaaat
    15541 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
    15601 tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc agcaactgat
    15661 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacatta gtcagacaca
    15721 gattatcggt atgttcatac agatgccgat catggtcgcg cacaaaacag accgtgccac
    15781 cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt tcgacaatca
    15841 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
    15901 tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg gtcatactgg
    15961 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
    16021 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcagg
    16081 aaatgcggtg agcatcacat caccacaatt cagcaaattg tgaacatcat cacgttcatc
    16141 tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc gaattcaggc
    16201 gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt tcgcaattgt
    16261 gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tgacgcgtta cgagcgtgaa
    16321 tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca tagtcagaac
    16381 ggctatatca cctgggatgt ggatagcctt gaaagtgcca ttcgccttgg attaaacaag
    16441 gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg cgtggacttt
    16501 gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg ttgcttatcg cgacagccgc
    16561 accaatggcc tgatgacgca ggcacaacag caactcggca aacgcgatat ttatcagcgt
    16621 agcggcatcc agtttctgcc cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa
    16681 caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta cttcagttat
    16741 cgcctgaccg gcaagatgaa ctgggaatat accaacgcca cgaccacgca actggtcaat
    16801 atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg
    16861 tttggtcgcc cgacgcatcc gggtaatgtc ataggtcact ggatttgccc gcagggtaat
    16921 gagattccag tggtcgccgt tgccagccat gataccgcca gcgcggttat cgcctcgccg
    16981 ttaaacggtt cacgtgctgc ttatctctct tctggcacct ggtcattgat gggcttcgaa
    17041 agccagacgc catttaccaa tgacacggca ctggcagcca acatcaccaa tgaaggcggg
    17101 gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct tcagcgagtg
    17161 cttcaggagc ggcaaatcaa cgatcttccg gcgcttatct ccgcgacaca ggcacttccg
    17221 gcttgccgct tcattatcaa tcccaatgac gatcgcttta ttaatcctga gacgatgtgc
    17281 agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccaa tcccggaaag tgatgctgaa
    17341 ctggcgcgct gtattttcga cagtctggca ttgctgtatg ccgatgtgtt gcatgagctg
    17401 gcgcagctac gcggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac
    17461 acgctgctca accagctatg tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt
    17521 gaagcctcga cgctcggcaa tatcggcatc cagttaatga cgctggatga actcaacaat
    17581 gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt tacccctaat
    17641 cctgacagtg aaattgccca ctatgtggcg cagcttcact ctacacgaca gacaaaggag
    17701 ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg
    17761 cggtggggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc
    17821 actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg
    17881 ggattcaggc tactggtaat tatccgggca aagcgcgtaa tgccagtgag ctacgagccg
    17941 atctggaaca ggctatgcgg ctgattccgg ggccgaaacg gcttaattta catgccatct
    18001 atctggaatc agacacgcca gtctcgcgcg atcagatcaa accagagcac ttcaaaaact
    18061 gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgtttttcgc
    18121 atccgctaag cgccgatggc tttacgcttt cccatgccga cgacagcatt cgccagttct
    18181 ggattgatca ctgcaaagcc agccgtcgcg tttcggccta ttttggcgag caactcggca
    18241 caccatcggt gatgaacatc tggatcccgg atggtatgaa agatatcacc gttgaccgtc
    18301 tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt gatcagcgag aagctaaacc
    18361 ctgcgcacca tatcgacgcc gttgagagca aattgtttgg tattggtgcg gagagctaca
    18421 cggttggttc caatgagttt tacctgggtt atgccaccag ccgccagacg gcgctgtgcc
    18481 tggacgccgg gcatttccac ccgactgaag tgatttccga caagatttcc gccgccatgc
    18541 tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac agcgatcacg
    18601 tggtgctgct ggatgatgaa acccaggcga ttgccagtga gattgttcgt cacgatctgt
    18661 ttgaccgggt gcatatcggc ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt
    18721 gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg
    18781 ctgagctgcg caagctggaa gcggcgggcg attacactgc gcgtctggcg ctactggaag
    18841 aacagaaatc gttgccgtgg caggcggtat gggaaatgta ttgtcagcgt cacgatacgc
    18901 cagcgggtag cgaatggctg gagaacgtgc gggcttatga gaaagaaatt ttgagtcgcc
    18961 gcgggtaaac actgccggat gcggcgcgag cgccttatcc ggcctacagg tcggcaacag
    19021 ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcgcg
    19081 agcgccttat ccggcctaca ggtcggcaac agttgtaggc ctgataagac gcgacagcgt
    19141 cgcatccggc attgattgcc ggatacggca c
//
LOCUS       DAZGCQ010000094        19048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000094 DAZGCQ010000000
VERSION     DAZGCQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.094"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..1555
                     /locus_tag="ACHK07_003732"
     CDS             29..1555
                     /locus_tag="ACHK07_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8104014.1"
                     /translation="MSKKFTKTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASSVIHGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEVLKQSD
                     FIASGPRPEGDPLVQASAVGEFALAVGEGAAAEEKNSTAVGSWASAHGRQSTALGSGG
                     QAYANGSTALGSYSHVDDSSVYGVALGGSSVVGENAPEGTALGSRAEVSHRNSVALGA
                     RAHTTRDNEVYIGYVEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNSDGTLTSADGATKTIAVNDGLVALSGRTDRIDA
                     AVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLF
                     QPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF
                     "
     gene            1642..2115
                     /locus_tag="ACHK07_003733"
     CDS             1642..2115
                     /locus_tag="ACHK07_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104015.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2486..6478
                     /locus_tag="ACHK07_003734"
     CDS             2486..6478
                     /locus_tag="ACHK07_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO8104016.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVI
                     LAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFN
                     RLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDG
                     GADISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPADTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDA
                     ETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     SSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGAGLYASALFDSG
                     AHIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFRYSF"
     gene            complement(7045..9717)
                     /gene="rcsD"
                     /locus_tag="ACHK07_003735"
     CDS             complement(7045..9717)
                     /gene="rcsD"
                     /locus_tag="ACHK07_003735"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO8104017.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVNIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            10456..11583
                     /gene="ompC"
                     /locus_tag="ACHK07_003736"
     CDS             10456..11583
                     /gene="ompC"
                     /locus_tag="ACHK07_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8104018.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            11695..12750
                     /gene="apbE"
                     /locus_tag="ACHK07_003737"
     CDS             11695..12750
                     /gene="apbE"
                     /locus_tag="ACHK07_003737"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO8104019.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            12824..13888
                     /gene="ada"
                     /locus_tag="ACHK07_003738"
     CDS             12824..13888
                     /gene="ada"
                     /locus_tag="ACHK07_003738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO8104020.1"
                     /translation="MKNATRLTEDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANAQQHRLDKITRACRLLEQEMPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRYGGENLAVRYALADCEQGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            13888..14538
                     /gene="alkB"
                     /locus_tag="ACHK07_003739"
     CDS             13888..14538
                     /gene="alkB"
                     /locus_tag="ACHK07_003739"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO8104021.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            14614..16257
                     /gene="yojI"
                     /locus_tag="ACHK07_003740"
     CDS             14614..16257
                     /gene="yojI"
                     /locus_tag="ACHK07_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO8104022.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            16475..18121
                     /gene="mqo"
                     /locus_tag="ACHK07_003741"
     CDS             16475..18121
                     /gene="mqo"
                     /locus_tag="ACHK07_003741"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO8104023.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(18497..18985)
                     /gene="eco"
                     /locus_tag="ACHK07_003742"
     CDS             complement(18497..18985)
                     /gene="eco"
                     /locus_tag="ACHK07_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO8104024.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         4612 a         4406 c         5340 g         4690 t
ORIGIN      
        1 atacagataa tttaacagga tatccataat gagtaaaaaa ttcacaaaga cacttctgtc
       61 atcttctctg ggtgggcttc tgcttgggat cagctcaggt gttttagctc aggagttacc
      121 tcctatcaaa gattctggtt tgccttttta ttcagtgctg aaagaggcag atgcttcgtc
      181 tgttatacac ggtagtactt atcgtattta ctataacaaa tcctctggtt ggggaacaat
      241 gcgatatgtt ctgaacggaa aagatgagac cctttttaat ttcgatcagg agggtaatat
      301 tatagtattg agtaaagata actcaattgc atataccgtt catgagcctg tgctaaaaga
      361 cttcgcccgt atggcagcag gcctcagaac gtctgacaaa gaaaacggta agcatcaggt
      421 tgatgaggag gaggtccgcc ggatcttcaa taaagttaat aatctatcaa atactattat
      481 taatcctgaa gtattaaaac aatcggattt catcgcatca ggaccacgtc cggaaggtga
      541 ccctttggtt caggctagtg cggtgggcga attcgctctg gctgttggag aaggtgcagc
      601 ggcggaggaa aagaattcaa cagctgttgg ttcatgggct agtgctcatg ggagacagag
      661 tactgcatta ggaagtgggg ggcaggctta tgctaatggt tcaaccgcgt tgggaagtta
      721 ttctcatgtg gatgattcat ccgtttatgg cgtggcatta gggggcagct ctgttgtggg
      781 tgaaaatgcc cccgaaggca ctgcactggg aagcagagcg gaagtttctc atagaaatag
      841 tgttgcttta ggggcgagag cgcacactac ccgtgataat gaagtttata tcggctacgt
      901 agaagaagcg ggcaaggctt ataaaactcg tgtgcttggt ggcctgagtg acggtactcg
      961 tccctcagat gcagcaaccg ttcgtcaggt tgaccgtgta aaagacagtg ttgagcaact
     1021 ggctcaggat acgaataccc gccttgtagt cgaagcgaaa aagtctcgtg aatatacaga
     1081 ttccagaacg acagttggtg tgaactctga cggaaccctg accagcgctg atggcgctac
     1141 aaaaacgatt gctgttaatg acggtctcgt tgctctgtcc ggcagaacag accgtattga
     1201 tgctgcagtt ggtagcgttg accgccgggt tactaaaaat acacaggcta ttcagtccaa
     1261 tactcgccag ttgcaggaac ataatgcacg cctgaacagc cagcagcgtc aaattcgtga
     1321 gaatcacgaa gagatgaagc gtgcagcagc acagagtgca gcgctggcgg ggcttttcca
     1381 gccgtacagt gtggggaaat tcaacgccac tgctgccctg ggggggtaca gtgataaaca
     1441 ggctgttgcc gtgggtgttg gttaccgttt caatgagcag acagcagcga aagcaggtgt
     1501 ggcagcgagt gatggcgatg tgtcctacaa cgtaggtgtg aactttgagt tttaattgct
     1561 gacagtgaca tgaagcatga cggcaggcgt ggagttttgc ctgccgtttt ttctcatgtg
     1621 agcatgaaac aggtgacagc gatgaaagtg attctggcga cccgcaaccg ttatctggag
     1681 tatggcctgc agcagatgct tgaggggtac aggataatac tggcgaggga gttttttaca
     1741 ccagagaaca ggaagagtgt accggcacat gatgagtcat gggtgataat ctgtgatgcc
     1801 ctccttggca ggctgatgtg ctgcatgttt cagggacgcc gttatcttca gattgatgcg
     1861 gaagatgtta cggggcgcct ggagacgtac aggaaaatcc ggaatggtga gtgggtacat
     1921 aatacgtatg cgcgacctct gacgatgtcc gagatggtgg tgatgtttgg atacgtgtac
     1981 cgggagtcaa agccctgcca tcttgcccgt gaaatgggga taaacacgaa gacggtgaac
     2041 acctttctgt atatgggact tggtaaaaat ggtcttaagt acaggagtgt gaagcacctg
     2101 gttggacgtg cgtagccgga atgcatattc gtgagtcggg taatgagagt ggaacggtgg
     2161 cagtgagaat ggaacagaaa aaaatcagat gcggaaagcg gaaatacaca gcgggtgcga
     2221 tgtgcttcat gctgtttgta tggctggggg gtgtggtgac attctgtgtg ctggccgctc
     2281 tgataatgag gtgaaaacgg aagagagttt acttccttaa ttcgaacttt ctgataactg
     2341 agaacttgtt aaatgcctgt gggtttatgt atttaaatca attggttaat ggtgatttta
     2401 tctttatcag tcaaagttcg ttttagtatg atgattgtgc ccgctgttta ttgttgtccg
     2461 ggagaaaata cagggaatcc aggggatgaa caaaatatac tcactcagag ttaatgacag
     2521 aaaagaactg gttgctgtac cggaaacggt ggggggcagg aaaaagtcgt ccggaattcc
     2581 cggtgcgggg atgctttccc gtcttcttct ggcctcagga gccgttgccg gcgttctgtt
     2641 ttcttctcct gcgctggcat ccgttgtcgg gaatactctg ccatggcaga cctacaggga
     2701 ttttgctgaa aacaaagggg cgtttcatgc cggggccaca aatattccgc tttatggtaa
     2761 gaatggggct gtcagtgggc ggctggacaa ggctccgatg atggatttca gtgttgtgga
     2821 ccagatactg ggggtggcaa cactgatttc tccgcagtat gtggcggggg ttaagcataa
     2881 cggcagctac aatacggtgc gttttggtta tgcggacgat acgacatatc gccttgttga
     2941 ccgtaatgag cactggcgtg attttcatac tccccggctg aacaaactgg tgacagaagt
     3001 tgccccggtg agcgtcacgg atgccggaac cggcaagggg gtataccaga accgcagtcg
     3061 ttatccggtg ttttatcgta tggggagtgg aactcagtac accggagctg cttccggtgc
     3121 gctgaccaga atagcggatg cttacgtctg gaggactggc gggacggtgg gaagtccggt
     3181 catttctgac tggagtctgg tgtcaaaccc gggaaatctt taccagggtg ttaatggtcc
     3241 gctggcgtct tacgggacat caggagacag tggctcaccg ctttttgcct gggatgcggt
     3301 gaagaagcag tgggtatttg ttgctgtact tcatggttat gccggtgaga acgggaagac
     3361 gaactggttt acggtgattc ctgcagggga tgtgaataac acgataaagc aggacaattc
     3421 aggcacagtt gtgccggcag ttgccggtgg tgacattgtg tggaagtaca gtcagggaag
     3481 cggagaggga accctttctc agggcgggca ggtatggaag atgaacggct tcaggggagg
     3541 gagtctgaat gacgggaaag atatcacctt tgggggtaag ggcacggttg ttctgaagga
     3601 tgatgttgtc cagggggcgg gttcgctcac gtttaatggt gattatacgg tcagaccgga
     3661 agggaatcag acctgggtgg gcgggggaat catcgtgaac gacgggcacc gtgttgactg
     3721 gatggtgaac ggacttgcgg gagacgcact tcataaaatt ggtaagggaa cactggtggt
     3781 ggccggcagt ggtgaaaatc ctggcaccct gaatacgggg gacggaacgg tcattctggc
     3841 acagaaggct gatgctgcag gcagggtccg ggcattcagt gaagtcagta ttgtcagcgg
     3901 acgaccagtt gtggtgctgc aggacagtca tcagattgaa ggggaccgca ttcgctgggg
     3961 ataccgggga ggtacgcttg atatcaacgg aaatgacata gccttcaacc gcctggctgc
     4021 ggctgatgag ggggcagtac tcacaagccg ggcgcgtcct gccacagtcc gtctggattt
     4081 cagccagtca gggcagaagg ccgtgatgtg gcatggtcat tttaccggta acctcagcgt
     4141 cctgaacaac acgtcatctg cggtggattt tatcatggat ggcggagcgg atatatccgg
     4201 cagttttaca cagcagggcg ggggactgta tatccagggg catccggttg ttcatgctgt
     4261 cagcagtgag gcggttgcga ctgcattgcg aaagcagggt gataattctg ttctgacgca
     4321 gccggtatca tttgctcaga aggactggga gagcagaacg ttcagcatcg gtcaactgaa
     4381 actgaagggg gcggcattca gcctttcccg caatgcgaca ctgacgggag atattgatgc
     4441 tgataatgcc accatggtgc tgggaagtga ctcgctatat ctggatatga aggacggcac
     4501 ggggagcagc agtgcgccgg ttaaggggac gtctgccgtg ggcggtgcat ccggtagcag
     4561 tacatttcgc gggaatgtga atatgcgtca ttctgccctg acggtccgcg accattttac
     4621 tggcagtata acggcatcgg acagccggat agctgtgagt tcagagaatg tgcgtctgga
     4681 agggaattcc cgcctgacct cttctgcact tactgtcagt gacggcgggc gtttacatgt
     4741 gaagggggga ctggagacag atggtggtgt gacactggac agaggaaccc ttttggtgga
     4801 cgggggaagt gtcaggaatg atgtatatga acgtctgctg gcctggtcag aggagcgtgg
     4861 cgggctgaac ggaagcggtg agtatgattt tatgacgggg gcagctggtc tcctgcggag
     4921 ttatgtcaga gggagtgcag gtaatgtgaa tctgcagaat gcggcctgga tgatgacggg
     4981 aaactcatcc gtgaagcatc ttgagtcatc gggaagtgct ctgtatttca gcaggccggg
     5041 cggggagttc cataccctga cggcaggcag tatggatatc agcgacagtg ttctggtgat
     5101 gcggacggac cttaaccact cagaccagct tcgggtgaca gaatcgctgc ggggtaagaa
     5161 taacctgttg ttggttgatt ttactgagcg ttctgacggg cagaaggcgc tgaatatccc
     5221 tctggtgacg gcgccggccg ataccggtgc ggatgtgttc agtgtgaaaa cgcgtgatac
     5281 cggcttcagt catatcacac ctgttgtcag ggctgaacag gggacgggcg gcacggcctg
     5341 gcagctgaat gttgtgcagc cggaaactgc cgcagagcct gtggcgacgc ggcaggatgc
     5401 agagacgccg aatcctgtgg aagctgtatc accgctccct tcaccggttt ctgctccgtc
     5461 atcggctccg gaggcgtcag tgacggatgt actgaccgga gagaatgcgg gggaatcagg
     5521 tgagtaccgt gatgagaccc ggtggctgct gacaggatac cggagtacgg ttaatagctc
     5581 cgcggtcagg gatgcgggga tgctgatgtc aatggggcac cgtaacttca ttaatgaggt
     5641 caacaacctg aacaaacgta tgggtgacct gcgggacatc aacggcgagg ccggtgcatg
     5701 ggcccgtatc atgagcggaa ccggttctgc cggcggtggc ttcagtgaca actacacgca
     5761 cgttcaggtg ggtgcggata aaaaacatga actggacggt ctggacctct tcaccggggt
     5821 gaccatgaca tatacctaca gcaacgccgg cagcgacacc ttcagtggta aaacgaagtc
     5881 agtgggtgca ggtctgtacg cttcagccct gtttgattcc ggggcccata tcgacctgat
     5941 tggtaagtat gttcaccatg ataatgagta tacggcgtcc tttgccggac tcggcacaaa
     6001 agactacagc tctcattcct ggtatgccgg tgctgaagtg ggttaccgct atcatgtcac
     6061 agaggatgcc tggattgagc cacaggcgga actggtctat ggtgctgtat ccggtaaaca
     6121 gttcagctgg aaggaccagg ggatgagcct gtccatgaag gacaaggact acaatccgct
     6181 gattggccgt accggtgtgg atgtgggtaa atcgttctcc ggtaaggact ggaaagtgac
     6241 ggcacgtgct ggtcttggct accagtttga cctgctggcg aacggagaaa cggtactgcg
     6301 tgatgcatcc ggagagaagc gtattaaggg cgggaaagac ggccgtatgc tgatgtctgt
     6361 gggtctgaac gcagaggtcc gtgacaacat ccgttttggt ctggagtttg agaaatcagc
     6421 ctttggtaag tacaacgttg ataatgctgt caacgccagc ttccgttatt cattctgacg
     6481 gttgttactg aaggggatta tggcctgacg gctccctgta aataaagaca ggcacagaag
     6541 attgtgagtt acttagtgtt ccaccaacaa gtaaggtcgt aactctgtgc ctgcttcgta
     6601 agcgtacagt gagggccatc gtcataactg aagcatccga cgacgtggtg tctaccagag
     6661 tgtcagatat gcaggaaaat atgagctccg acagaaatgc agtaaaaaaa gagagtctcg
     6721 tacacccctc cagaaaactc cagggctgta agttgtatta aaaagctgat ttttctttag
     6781 cgatacagac ctcaataagt ataacaccct tcagttactg tggttatgca caggtgttat
     6841 ttttaactgt gtggataaac agtgtcgatt attcagattg gttatggggc atgtatggga
     6901 catttttaat cggcgaaata cgtcgaagtt cgtcgatatg gtaaacgaat catctatcca
     6961 acccttgaaa aacggcgctc ctggacgatc ttcgtcgatt tttaaaatgc aacaagtacg
     7021 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     7081 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     7141 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     7201 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     7261 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     7321 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     7381 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     7441 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     7501 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     7561 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     7621 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggtc
     7681 agctgcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     7741 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     7801 cgccttgcca tagcgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     7861 ttcatgaatg tttacgcctt ctccggtgtc caggatgcgg aacgtcaggc ggtcttcgga
     7921 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     7981 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     8041 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     8101 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     8161 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     8221 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     8281 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg
     8341 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     8401 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     8461 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     8521 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     8581 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     8641 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     8701 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     8761 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     8821 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     8881 aatcttcgta ctgttaaagt ggatactgac gctatccgtc ccttctttct cattatcatt
     8941 gtctcccgtt gccgtcgcgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     9001 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     9061 aaaggtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     9121 aaagctttcg cgttcatcga gagcgttggc ctgttgcaac atctctgcac gacgtgaatc
     9181 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     9241 tgagatcagc accagactgt tatcctgacc attcaggtaa tacattgacc acggtacatt
     9301 ttctgcgccc cacaatgtgt ccagataggt ggacatccgt tgagtcatct caagcgttga
     9361 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgcggttttt ccagatagta
     9421 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     9481 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     9541 agcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     9601 ttgtaccatt acacccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     9661 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     9721 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     9781 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     9841 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     9901 cgaaaataat tgcaaataaa caaagattgc tggaaattat gctgatgtta attatttgtg
     9961 aaatggttaa caagcgttat agtttttctc tggtagcaca gaataatgaa aagtgtgtaa
    10021 agaagggtaa aaaaaaccgg atgcgaggca tccggttgaa ataggggtaa acagacattc
    10081 agaaatgaat gacggtaata aataaagtta atgatgatag cgggagttat tctagttgcg
    10141 agtgaaggtt ttgttttgac attcagtgct gtcaaatact taagaataag ttattgattt
    10201 taaccttgaa ttattattgc ttgatgttag gtgcttattt cgcgattccg caataatctt
    10261 aaaaagttcc cttttattta cattttgaaa catctatagc gataaatgaa acatcttaaa
    10321 agttttagta tcatattcgt gttggattat tctgcatttt tggggagaat ggacttgccg
    10381 actgattaat gagggttaat cagtatgcag tggcataaaa aagcaaataa aggcatataa
    10441 cagagggtta ataacatgaa agttaaagta ctgtccctcc tggtcccagc tctgctggta
    10501 gcaggcgcag caaacgctgc tgaagtttac aacaaagacg gcaacaaatt agatctgtac
    10561 ggtaaagtag acggcctgca ctatttctct gacgacaagt ctgtagatgg cgaccagacc
    10621 tacatgcgtc ttggcttcaa aggtgaaact caggttactg accagctgac cggttacggt
    10681 cagtgggaat atcagatcca gggcaacgct ccggaaagcg aaaacaactc ctggacccgt
    10741 gtggcatttg ctggtctgaa attccaggat gtgggttctt tcgactacgg tcgtaactac
    10801 ggcgttgttt atgacgtaac ttcctggacc gacgtactgc cagaatttgg tggtgacact
    10861 tacggttctg acaacttcat gcagcagcgt ggtaatggct acgcaaccta ccgtaacacc
    10921 gacttcttcg gtctggttga cggtctgaac tttgctgttc agtaccaggg caaaaatggt
    10981 agcgtaagcg gcgaaggcat gaccaacaat ggtcgtggtg ctctgcgtca gaatggcgac
    11041 ggtgtcggcg gatctatcac ttatgattac gaaggcttcg gtatcggtgc tgcagtttcc
    11101 agctccaaac gtactgatga tcaaaatggt agctacatca gcaatggtgt agttcgtaac
    11161 tacatcggta ctggcgaccg tgctgaaacc tacaccggtg gtctgaaata cgatgctaac
    11221 aacatctacc tggctgctca gtacacccag acctacaacg caactcgcgt aggcaacttg
    11281 ggttgggcga acaaagcaca gaacttcgaa gctgttgctc agtaccagtt cgacttcggt
    11341 ctgcgtccgt ccctggctta cctgcagtct aaaggtaaaa acctgggtgt catcaatggt
    11401 cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc
    11461 aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc
    11521 actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt ttaccagttc
    11581 taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta
    11641 caaactcagt tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa
    11701 ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa
    11761 aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg
    11821 ggtaccttct ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa
    11881 gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat
    11941 tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc cgtggccagt aagtgaagcg
    12001 atggccgaaa tcgtcactac ctcgctgcgc attggcgcga agaccgatgg tgcgatggat
    12061 attaccgtcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa
    12121 attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg
    12181 gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt atatgtcgat
    12241 ctctctaccg tcggcgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa
    12301 gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt
    12361 gaaggccagc cgtggcgggt agcgattcaa aagccaaccg ataaagaaaa tgcggttcag
    12421 gccgtggtgg atatcaacgg tcatggtatt agcacctctg gcagctaccg taactattac
    12481 gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa
    12541 cacaatctgg tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat
    12601 actggcttga tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg
    12661 gcggtttata tgatcaccaa agagggcgat agctttaaaa cctggatgtc gccgcagttt
    12721 aaaagcttcc tggtgagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg
    12781 tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacacg
    12841 cttaactgaa gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga
    12901 attcgttttc gccgtgcgca ccacaggtat cttttgccgt ccgtcttgcc gcgccagaca
    12961 tgccttgcgg gaaaacgttt ctttctatgc tgatgccagc gaggcgcttg ccgctggctt
    13021 tcgtccctgc aaacgctgcc aacctgataa agccaacgcc cagcaacatc ggctggataa
    13081 aatcacccgc gcttgtcgac tgctggaaca ggaaatgcct gtaacgctgg aggccttagc
    13141 tgaccaggtg gcgatgagtc cgttccatct gcatcgttta tttaaagcca cgaccggaat
    13201 gacgccaaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa
    13261 aggggagagc gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta
    13321 ccgcaaagcc gatgaaacgc tgggcatgac ggctaaacaa ttccgttacg gtggcgaaaa
    13381 tctggcggtg cgttacgcgc tggctgattg tgagcagggt cgttgcctgg tggcagaaag
    13441 cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacactaa tcagcgagtt
    13501 gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ctgacgtttc agcaacatgt
    13561 gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggatat
    13621 tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga
    13681 aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt
    13741 tgccagcgcc tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gagtggtccg
    13801 tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg
    13861 ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg
    13921 caagagccac tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa cgctgcggag
    13981 caactaatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcact
    14041 ccaggcggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgagc
    14101 catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc
    14161 atgccgcaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg ctatccagat
    14221 ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat
    14281 caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc
    14341 gcgatttttc aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa
    14401 catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg
    14461 ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag
    14521 gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac
    14581 tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg
    14641 cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc
    14701 attggcttaa ttgcttttat caaccagcgc cttatcgaaa cggcggatac cagtctgctg
    14761 gtgttgccgg agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa
    14821 ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag
    14881 cggattctgg atacccacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg
    14941 ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactggtg
    15001 caggggatca ttctcactat cggttcagcg gcgtatctgt ggatgctgtc gggcaaaatg
    15061 ttgctggtaa cggctatctg gatggcagtc accatctggg gcggttttgt gctggtggcg
    15121 cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag acaagctgta cacggatttt
    15181 caaactgtac ttgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg
    15241 tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat tcgcgcagac
    15301 accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc aatcggcctg
    15361 gtgttctgga tggcgaacag cctcggttgg gctgatacca acgttgccgc gacctattcg
    15421 ttgacgcttt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg
    15481 ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca
    15541 gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg
    15601 tttgcttatc aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc
    15661 gagctgctgt ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg
    15721 acgggcttgt atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc
    15781 gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt
    15841 gatcaactgc tggggccgga gggtaaaccc gctaacccgc aactggttga gaagtggctg
    15901 gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag
    15961 ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat
    16021 attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat
    16081 caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat cagtcatgat
    16141 gatcattact ttatccacgc cgaccgcctg ctggagatgc gcaacgggca acttagcgag
    16201 ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat
    16261 tacgacgctg acctggcgga ctgctgccgc caggtcaata tgctaaaaat cccttcattt
    16321 ctaataaccc tataattaat caacgaaatt ataatgtttc taaaattaga atataattta
    16381 taaacattat ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct
    16441 gcaataaagc gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt
    16501 ctcgatggcc gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga
    16561 gcaggaaact gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta
    16621 tttacgcgag ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggggtcgc
    16681 gcaggagagt tcgaacggct ggaacaacgc cggaaccggg cattctgcac tgatggaact
    16741 gaactacacc ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa
    16801 cgaagcattt cagatttccc gccagttctg ggcgcaccag gtcgagcgcg gcgtgctgcg
    16861 tactccgcgt tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa
    16921 cgtcaatttc ctgcgcgccc gttacgccgc gttgcaacaa agctcgctat ttcgcggtat
    16981 gcgttactct gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg
    17041 cgatccgcaa cagaaagtgg cagccacgcg tacggaaatt ggtaccgatg tgaactacgg
    17101 cgagatcacc cgccagttaa ttgcttcctt gcagaagaaa tctaacttct cgctgcaact
    17161 cagcagcgaa gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc
    17221 cgatctgaaa aatggcactg cacagaacat ccgtgccaaa tttgtcttta tcggcgcggg
    17281 cggtgcggca ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg
    17341 tttcccggtg ggcggtcagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct
    17401 ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga
    17461 tacccgcgtt ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac
    17521 caaattcctc aaaaacggtt cattgtggga tctaatgagt tccaccacca cctctaacgt
    17581 gatgccgatg atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca
    17641 ggtgatgttg agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaagcgaa
    17701 aaaagaggac tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc
    17761 cgataaaggt ggtgtactgc gtctgggtac tgaagtggtc agtgaccagc aagggaccat
    17821 tgccgcgctc ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct
    17881 gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc
    17941 gatcgttccg tcttatggac gcaagctgaa cggtgatgta gccgcaacag agcgcgagtt
    18001 gcagtacacc agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag cagatagtac
    18061 gccgaaaccg cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta
    18121 atgatgtgcc acatccggca tggtatgccg gatgtggcgt atgctgataa gacgcgccag
    18181 cgtcgcatca ggcaaccggc tcgggcgtta gatgttggat gcggcgtaac cgccttatcc
    18241 gacctacggg gtggagcact tcacagcggt gtaggcctga taagacgcgt cagcgtcgca
    18301 tcaggcaacc ggctcggacg ttggatgtcg gatgcggcgt tcccgcctta tccgacctac
    18361 ggggtggagc acttcacagc ggtgtaggcc tgataacacg cgtcagcgtc gcatcaggca
    18421 accggctcgg gcgttagatg ttggatgcgg cgtaaccgcc ttatccaacc tatggggtgc
    18481 cgcacttcac ggcagtttag cgaactaccg cgttgtcaat tttctcttcc gccttccaga
    18541 cgcggtactt cacatctaca ttgtctggcg tatacaccac gatcggcagc ttgctgttgt
    18601 aacgcagcat tccagcatcg cccagatacg cggtgacaaa tttcttctct ttcttgccat
    18661 ccgggcaggc catcatcgtg gaaaccggcg agctaacttt atcaaagaca taataatcat
    18721 agccccagcc ttccagcgtt ttgctttcca gcttcccgcc gagacgatgc aaattgcaat
    18781 cgacttccag cgtctgaccg attaacagtt ctactttcag ggtagattca tcttcttgcg
    18841 gggttaactg aatcacctga cgcttcatgc ctttttcagc ttgtggataa ggcgcgattt
    18901 tttccagtgg ctggacgctc tctgccgccc aggcggaagt ggtggcgaaa gcggcaaaca
    18961 atactgcagg tagaatggtc ttcatttttt gctttccttc ttcatttata ggtttgcacc
    19021 gatgatgtcg ctgaaggtta acatattc
//
LOCUS       DAZGCQ010000095        19022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000095 DAZGCQ010000000
VERSION     DAZGCQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.095"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..427
                     /gene="nudI"
                     /locus_tag="ACHK07_003743"
     CDS             2..427
                     /gene="nudI"
                     /locus_tag="ACHK07_003743"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8104025.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEIKPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(466..1068)
                     /gene="ais"
                     /locus_tag="ACHK07_003744"
     CDS             complement(466..1068)
                     /gene="ais"
                     /locus_tag="ACHK07_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO8104026.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1376..2515
                     /gene="arnB"
                     /locus_tag="ACHK07_003745"
     CDS             1376..2515
                     /gene="arnB"
                     /locus_tag="ACHK07_003745"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO8104027.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2519..3487
                     /gene="arnC"
                     /locus_tag="ACHK07_003746"
     CDS             2519..3487
                     /gene="arnC"
                     /locus_tag="ACHK07_003746"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO8104028.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3487..5469
                     /gene="arnA"
                     /locus_tag="ACHK07_003747"
     CDS             3487..5469
                     /gene="arnA"
                     /locus_tag="ACHK07_003747"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO8104029.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPVCTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5466..6356
                     /gene="arnD"
                     /locus_tag="ACHK07_003748"
     CDS             5466..6356
                     /gene="arnD"
                     /locus_tag="ACHK07_003748"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO8104030.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6356..8008
                     /gene="arnT"
                     /locus_tag="ACHK07_003749"
     CDS             6356..8008
                     /gene="arnT"
                     /locus_tag="ACHK07_003749"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO8104031.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8005..8340
                     /gene="arnE"
                     /locus_tag="ACHK07_003750"
     CDS             8005..8340
                     /gene="arnE"
                     /locus_tag="ACHK07_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO8104032.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8340..8726
                     /gene="arnF"
                     /locus_tag="ACHK07_003751"
     CDS             8340..8726
                     /gene="arnF"
                     /locus_tag="ACHK07_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO8104033.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8720..8986)
                     /gene="pmrD"
                     /locus_tag="ACHK07_003752"
     CDS             complement(8720..8986)
                     /gene="pmrD"
                     /locus_tag="ACHK07_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO8104034.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(9096..10451)
                     /gene="menE"
                     /locus_tag="ACHK07_003753"
     CDS             complement(9096..10451)
                     /gene="menE"
                     /locus_tag="ACHK07_003753"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO8104035.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10448..11410)
                     /gene="menC"
                     /locus_tag="ACHK07_003754"
     CDS             complement(10448..11410)
                     /gene="menC"
                     /locus_tag="ACHK07_003754"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO8104036.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRG
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11410..12267)
                     /gene="menB"
                     /locus_tag="ACHK07_003755"
     CDS             complement(11410..12267)
                     /gene="menB"
                     /locus_tag="ACHK07_003755"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO8104037.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12282..13040)
                     /gene="menH"
                     /locus_tag="ACHK07_003756"
     CDS             complement(12282..13040)
                     /gene="menH"
                     /locus_tag="ACHK07_003756"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO8104038.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQRLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13037..14707)
                     /gene="menD"
                     /locus_tag="ACHK07_003757"
     CDS             complement(13037..14707)
                     /gene="menD"
                     /locus_tag="ACHK07_003757"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO8104039.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14796..16091)
                     /gene="menF"
                     /locus_tag="ACHK07_003758"
     CDS             complement(14796..16091)
                     /gene="menF"
                     /locus_tag="ACHK07_003758"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO8104040.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVFARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16170..16475)
                     /gene="elaB"
                     /locus_tag="ACHK07_003759"
     CDS             complement(16170..16475)
                     /gene="elaB"
                     /locus_tag="ACHK07_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO8104041.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16530..16991)
                     /locus_tag="ACHK07_003760"
     CDS             complement(16530..16991)
                     /locus_tag="ACHK07_003760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8104042.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            17056..17973
                     /gene="rbn"
                     /locus_tag="ACHK07_003761"
     CDS             17056..17973
                     /gene="rbn"
                     /locus_tag="ACHK07_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO8104043.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18161..>19022
                     /locus_tag="ACHK07_003762"
     CDS             18161..>19022
                     /locus_tag="ACHK07_003762"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO8104044.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLNSHDSDNLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEISCYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRSLGHEKENSLKRIINSF
                     NSFNCDPTRET"
BASE COUNT         4591 a         5033 c         4741 g         4657 t
ORIGIN      
        1 tgtgcgacaa cggactattg tatgcccttt gattcaaaat gatggtgctt atttgctgtg
       61 taaaatggcc gacgatcgcg gcgttttccc cggtcaatgg gcgctttcgg gtggcggcgt
      121 ggagcccggc gaacgaattg aagaggcact acgccgcgaa attcgcgaag aactgggaga
      181 acagctgctt ttgacagaaa tcaagccgtg gaccttcagc gatgatattc gcaccaagac
      241 gtatgcagat ggtcgcaagg aagagattta tatgatttac ctgatttttg actgcgtttc
      301 tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac tacgcgtggg taaaacctga
      361 agatctggtg cattatgatt tgaatgttgc cacccgaaaa acgttacgtt tgaaaggtct
      421 tctttaacaa tgatgacagc catcactggc tgtcagattg aaaatttagt ggttaacgaa
      481 ttccccatcc agataaactt tgcctttttc cacatgcatg actaaaccat ccagataatc
      541 aggtttaaat gtcgcatcac gcttatcttt agcaatatat gtcaggcaat gattatgggt
      601 gaaaataacg atatttttat caggcgcttt gctttgtaag tccttaattg cactataaat
      661 ctcattaccg cactgaagaa gtcgtttatc taccgtcaat tttttacccg ctgaaaacca
      721 ggtcgccgac tgaatggtcc ggacggtatt actggaatag agatcgaaat cagggatatc
      781 agcactaaaa gcgttgccca gttctcgggc atcctgggta cctttgaccg taatacctgt
      841 tttatctgac aagcattgat tggttgaacg atcgcaacgt tcagcatggc gaaacaaaac
      901 gacaaccggg tgctgctgtg ccagtctggc cagtgtttta ttgtcgatac gtggcaaacc
      961 attgctactc caggcggcat gagtacccag tccggcaatt gcagcgagcg ccagtaaaat
     1021 gatgaaatat ttttttgact tcaatgatga gcggcaaaaa gctaacacgt aaactccacc
     1081 tatagacaag cgcaaccaga caattaccgt gaaattgagc gacatttctg gcgataatta
     1141 gcagttggtg taatattaaa aatcctacga tgtcggcaaa atgcctcaaa attttgccaa
     1201 atgcaaagcc taaataagaa aaaatataaa aatttcaata tttacgtcta atattagttt
     1261 cttaaggtta agttaatatt ctatccttaa aatttcgctc caaatggcaa aatatacaca
     1321 acactcttta tagcaaatat aagtggacag gtattcaatg gcggaaggaa aagcaatgtc
     1381 agaatttttg cctttttcgc gaccagcaat gggcgtggag gaactcgctg cagttaaaga
     1441 ggttctcgaa tccggttgga tcacaaccgg tccgaagaat caggcgcttg agcaagcctt
     1501 ttgccagttg acgggaaatc agcatgccat cgcggtcagt tcagccaccg ccggaatgca
     1561 tatcacgcta atggcgttgg aaattggcaa gggcgatgaa gtgattacgc cttccctgac
     1621 ctgggtttca accctcaata tgatttcctt gttgggtgca acgccggtaa tggtggatgt
     1681 cgatcgcgat acgctgatgg tcacgcctga agcgatcgaa tcagccatta cgccacgcac
     1741 taaagccatc attccggtgc attatgccgg tgcgccagca gatattaacg ccattcgcgc
     1801 cattggcgaa cgttacggca tcgcagttat cgaagatgca gcccatgccg tcggtacgta
     1861 ttacaaaggg cgacatattg gcgcaaaagg taccgctatt ttttcgtttc acgccatcaa
     1921 aaatattacc tgtgcggaag gtggcctgat tgttactgat aatgaaaatc ttgcccgcca
     1981 gctacggatg ctgaaatttc acggtctggg tgtcgatgcc tatgacagac aaacctgggg
     2041 ccgtgcaccg caggctgaag tcttaacacc gggctataag tacaatctga ccgatattaa
     2101 cgccgcgatt gccctgacac agttagctaa attagagcac ctcaacaccc gtcggcgcga
     2161 aattgcccag caatatcagc aagcactggc agctctcccc tttcagccat taagccttcc
     2221 cgcctggtcg cacgttcacg cctggcatct gtttattatt cgtgtcgatg aacaacgttg
     2281 tggtatcagt cgaaatgcgt tgatggaagc gttaaaagaa agaggtattg gtaccgggtt
     2341 acatttccgc gccgctcaca cacaaaaata ttatcgcgag cgttttccca cgctgtcgtt
     2401 accgaatacc gaatggaata gcgaacgcat ctgttcgttg ccgctgttcc cggatatgac
     2461 taccgccgat gccgaccgtg tcatcacagc ccttcagcaa ctcgcaggac aataagccat
     2521 gtttgaaatc caccctgtta agaaagtctc ggtggttatt cccgtttata acgagcagga
     2581 aagcttaccg gaattaatca ggcgcaccac cacagcctgt gaatcgttgg ggaaagagta
     2641 tgagatcctg ctgattgatg acggcagtag cgataattcc gcgcatatac tggtcgaagc
     2701 ctcacaagcg gagaacagcc atattgtgtc tattttgctt aaccgcaatt acgggcaaca
     2761 ttcagcgatt atggcgggat tcagtcacgt tactggcgac ttaattatta cccttgatgc
     2821 cgatctccag aatccgccag aagaaatccc ccgcctggtg gcaaaagccg atgaaggtta
     2881 cgacgtggta gggactgtac gccagaaccg ccaggacagc tggtttcgca aaaccgcttc
     2941 gaagatgatt aaccggctta ttcagcgcac caccggcaaa gcgatgggtg actacggttg
     3001 tatgttgcgc gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc atgaacgcag
     3061 cacctttatc ccgattctgg cgaatatctt cgcccgccgt gccattgaaa ttccagtgca
     3121 tcatgccgag cgtgagtatg gtgaatccaa atacagcttt atgcgcctga ttaatttgat
     3181 gtacgacctg gtgacctgcc ttaccacaac gccgctacgt atgctgagtc tgctcggcag
     3241 cattattgcg attggaggtt ttagcattgc ggtgctgctg gtgattttac gcctgacctt
     3301 cggaccacaa tgggcggcag aaggtgtctt tatgctattt gccgtgctgt ttacttttat
     3361 tggcgctcag tttatcggca tgggattgct cggtgaatat atcggcagga tctacaccga
     3421 tgtccgcgcc cgcccccgct attttgttca gcaagttatc cgtccatcca gcaaggaaaa
     3481 tgaataatga aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg tattgaagcc
     3541 ctgctggctg ccggttacga aattagcgcc atttttaccc ataccgataa tcccggtgaa
     3601 aaagcctttt atggttcggt ggctcgtctg gcggcggaaa gaggcattcc ggtttatgcg
     3661 ccggataacg ttaatcatcc gctgtgggtg gaacgcattg cccaactctc accagaggtg
     3721 attttctctt tttattatcg ccatcttatt tgcgacgaga ttttgcagct cgctcccgca
     3781 ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac
     3841 tgggtgctgg tgaacggtga aacagaaact ggcgttacat tgcaccgaat ggtaaaacgt
     3901 gccgatgccg gggccattgt ggcgcaactg cgcgttgcca ttgcgccaga cgatatcgct
     3961 attacgctgc atcataaatt gtgccatgcc gcgcgccagc tactggaaca gacattaccc
     4021 gccattaaac acggtaatat tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt
     4081 ggtcgcagaa cgccggatga cagtttcctc gaatggcata agccggcatc cgtactgcac
     4141 aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag
     4201 aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca gcaaagcaca gccggggagc
     4261 gtgatttctg ttgcgccact gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc
     4321 ggacaggcgg gcgacggcat tactatgcag ggctcgcaat tagcgcagac gctgggcctg
     4381 gtgcaaggtt cacgcttgaa tagccagcct gtctgcaccg cccgacgccg tacccgggta
     4441 ctcatcctcg gggtgaatgg ctttattggc aaccatctga cagaacgcct gctgcgcgaa
     4501 gatcattatg aagtttacgg tctggatatt ggcagcgatg cgataagccg ttttctgaat
     4561 catccgcatt ttcactttgt cgaaggcgat atcagtattc attccgaatg gattgagtat
     4621 catgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga tagccacgcc gattgaatat
     4681 acccgcaacc cgctgcgcgt atttgaactc gattttgaag agaatctgcg cattatccgc
     4741 tactgcgtga agtaccgtaa gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg
     4801 tgtagcgata aatacttcga tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa
     4861 ccacgctgga tttattcggt atcaaaacaa ttacttgatc gggtgatctg ggcctatggc
     4921 gaaaaagagg gtttacagtt caccctcttc cgcccgttta actggatggg accgcggctg
     4981 gataacctta atgcagcgcg aattggcagc tcccgcgcga ttacgcaact cattctcaat
     5041 ctggtagaag gttcaccgat taagctaatt gatggcggaa aacaaaaacg ctgctttact
     5101 gatattcgcg atggtatcga ggcgttatac cgcattatcg aaaatgcggg aaatcgctgc
     5161 gacggcgaaa ttatcaacat tggcaatcct gagaacgaag cgagcattga ggaactgggc
     5221 gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc atcatttccc accgtttgcg
     5281 ggctttcgcg ttgtcgaaag tagcagctac tacggcaaag gatatcagga cgtagagcat
     5341 cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact gggagccgaa aattgatatg
     5401 caggaaacca tcgacgaaac gctggatttc ttcctgcgca ccgttgatct tacggataaa
     5461 ccatcatgac caaagtaggc ttacgcattg atgtcgatac ctttcgtggc acccgtgaag
     5521 gcgtgccgcg tctgctggaa atcttgagta agcataatat tcaggccagc atttttttca
     5581 gcgtcggccc ggacaatatg ggccgtcatc tctggcgact ggtgaagcca cagtttttgt
     5641 ggaagatgct gcgctcaaac gcggcatcgc tttatggctg ggatatttta ctggcaggta
     5701 cggcctggcc aggtaaagag attggtcatg ccaatgccga tatcattcgt gaagcggcta
     5761 aacatcacga agtcggcctg cacgcctggg atcaccatgc ctggcaagcc cgtagcggta
     5821 actgggatcg gcaaacaatg atcgacgata ttgcacgcgg ccttcgcact ctggaagaga
     5881 ttatcggtca accggtaacc tgttctgccg ctgcgggctg gcgtgccgac cagcaggtga
     5941 tcgaagcaaa agaagcgttc catttgcgct acaacagcga ttgtcgtggg gccatgccgt
     6001 tccgtccact gctcgaatca ggaaaccctg gcactgcgca aattccggtg accttaccca
     6061 cctgggatga agtgattggt cgggatgtga aagcagaaga ttttaacggt tggttactca
     6121 accgcatcct gcgagataaa ggcacgccgg tttataccat tcatgcagaa gttgaaggct
     6181 gcgcttatca gcataatttt gtggatctcc tcaaacgcgc agctcaggaa ggcgtgacat
     6241 tttgcccttt aagcgaactg ttatcagaga cgttgccgct cggacaagtt gttcgcggaa
     6301 atattgccgg acgtgaaggc tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa
     6361 atcggtacgt taccttatcg gcctcttcgc gtttattgcc tgctattacc tgttaccgat
     6421 cagcacgcgt ctgctatggc aacccgatga aacgcgttat gcggaaatca gtcgagaaat
     6481 gctggcatcc ggcgactgga ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc
     6541 cattgccgga tactggatta acagcattgg gcaatggcta tttggcgcga ataactttgg
     6601 tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc gcgctggtga cctggtttac
     6661 tctgcgctta tggcgcgata aacgtctggc tctactcgcc acagtaattt atctctcatt
     6721 gtttattgtc tatgccatcg gcacttatgc cgtgctcgat ccgtttattg ccttctggct
     6781 ggtggcggga atgtgcagct tctggctggc aatgcaggca cagacgtgga aaggcaaaag
     6841 cgcaggattt ttactgctgg gaatcacctg cggcatgggg gtgatgacca aaggttttct
     6901 cgcccttgcc gtgccggtat taagcgtgct gccatgggta gcgacgcaaa aacgctggaa
     6961 agatctcttt atttacggct ggctggcggt tatcagttgc gtactgacgg ttctcccctg
     7021 gggactggcg atagcgcagc gggagcctga cttctggcac tattttttct gggttgagca
     7081 tattcaacgc tttgcactgg atgatgccca acatagagct ccgttctggt actacgtgcc
     7141 ggtcatcatt gccggtagcc tgccgtggct gggattactc cccggtgcac tatacacagg
     7201 ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg agctggacga taatgccgct
     7261 gctgtttttc tccgttgcta agggtaaatt gcccacctat attctttctt gctttgcacc
     7321 tttggcaatg ctgatggcgc attacgcttt gctggcagca aaaaataatc ctctggcgct
     7381 gcggattaat ggctggatta acatcgcttt tggcgtcact ggcattattg ccacatttgt
     7441 ggtctccccc tggggaccaa tgaacacgcc ggtgtggcaa accttcgaga gctataaagt
     7501 cttttgtgcc tggtcgattt tttcgctatg ggcatttttc ggctggtaca ccttaacaaa
     7561 cgtcgaaaag acttggtctt ttgccgcgct ttgcccgctg gggctggcgt tgctggtagg
     7621 attttcaatt cctgacagag tcatggaagg aaaacatccg caattttttg tcgagatgac
     7681 acaagaatca cttcagccaa gccgctatat tcttactgat agcgtcggtg ttgccgcagg
     7741 tctggcatgg agcctgcaac gcgatgacat catcatgtat cgccagacag gtgagttgaa
     7801 atacggcctt aattatccgg atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa
     7861 ctggcttaat caacatcgtc aggaggggat tattacactc gtgctttcgg ttgaccgcga
     7921 tgaagatatc aacagtctcg ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct
     7981 ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat tagtctttgc cagcttgctt
     8041 agcgttgccg ggcagttgtg tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg
     8101 cgcaaacata tcgtgctgtg gctgggactg gcgctggctt gtcttggtct tgccatggtg
     8161 ctctggctgc tggtcttgca gaacgtaccg gtaggcattg cctacccgat gttaagtctg
     8221 aattttgtct gggtgacgct ggctgcagta aaactgtggc acgaaccggt atcgccgcgt
     8281 cactggtgtg gggtggcgtt cattattggt ggcattgtga tcctcgggag tacggtgtaa
     8341 tgggcctgat gtggggatta ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg
     8401 gttttgcggc gagtcatctg ccgccgatga cgcacttatg ggattttatc gcggctctgt
     8461 tagcttttgg gctggatgcc cggatactgt tactcgggct gctgggttat ctgctttccg
     8521 ttttttgttg gtataaaacg ctacataaac ttgcgctaag caaagcctat gccttgctca
     8581 gtatgagtta tgtgctggta tggattgctt caatggtact tcccggctgg gaagggactt
     8641 tttcgctgaa agcactactg ggagtagcct gtattatgag cgggttgatg ctgattttcc
     8701 tgcccacgac aaaacaacgt tactgagttt tccctgccac tttacactgc cgttcccact
     8761 catcggggct ataacagctt gcagagagga ctttcaccgt atgcagcttt tcacgattaa
     8821 tacaataaag cgcattctgc aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac
     8881 tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc gcagagcata agtacatgtc
     8941 tgttatcttg tttattgcag cacgattttt tgaccagcca ttccattgta ttatcctgtt
     9001 tgctaagagt tttcatatca atttacagat taacagataa actctttatc aacggcaatg
     9061 gaatggcaga actgagaagt gaaacggagg gttaattatt gctgacgttg cacccactct
     9121 tttagcgcct gacgtgaaat tttaataccg ccgtttttca gctccggcgg caaagtcagc
     9181 caacgtaccg gttgctgaaa acgggctagc ttatctttca cccattcact aagatcaacg
     9241 ctctcgtggt catactccat caccgccacc ggtcgatgac caaactcctt gtcggcaacg
     9301 gggacgataa acacctgcaa aaccgcagga tgtgcagcaa ttacgcgctc gacttcttcc
     9361 ggctgaatac cctctccgcc actgaagaat agattgtcta aacgtccgac aatggtcagc
     9421 ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt
     9481 gaaaccagtt gcccgttacg ccagtaacct tctgccatac tggcagcccg cagccacact
     9541 tcattattaa cgattttcac ttcccgaccc ggcagcggcg aaccaacgtc tgccaggccg
     9601 tcggcttctt tcgcacacac cgtggaggca aactcggtca gaccatagcc gcaaaagcaa
     9661 cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc accgccaagt
     9721 aacaccgctt tcagggaaac ggaactgcgg ttaaccagca aacgccagag ttgtgttggc
     9781 accagtgaag cgtgagtaca gcctgccagc atttgctcca atggctgttt atcacgcacc
     9841 gtcatccgcg caccagcgta taaccagcgc cacataattc cctgaccgga gacgtgaaac
     9901 agcggtaaag agagcaacca gtcatcgtga tcgccaaacg gaatcaacga taacacccct
     9961 tcggcactgg caagatgggc ctgataagta tggacagcgg ctttcggcaa cccggtagaa
    10021 cccgaggtca acgtcattga gcacagacgc gtcggctgcc acgcagcggc atgtgcgcct
    10081 tcaaccagct gaatgtgcag cgacgttaac gccggaaacg tgttttcccc atccggcacc
    10141 agcgcaaatt gcagcgtcag attgggcagc aattcttcaa gcaacggttg cggcagctga
    10201 gggttcacgg gcaacacccg cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt
    10261 tgcggcgtat tccacgcccg caacatcacg ccgctgccct caaccacacc ctgcaccgca
    10321 aatccggagg ctaattcatc gacgcgagca caaagctcgc gccagttgag ttgctcgtca
    10381 ttaagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca cggccagtca
    10441 gagaagatca taacaaccgc tccagtgcat caacttccac gacaggcagc gtgctacccg
    10501 gccagcgacg tacctgctgc gcctgcatca gatccagcgt gtccagccct ggaatggtgt
    10561 ccggcgttaa ccaggcggca atccgcgcca gttgcgttaa gcctaagctc gattcaatgg
    10621 aagaactgat caccgccgtc agccccagcg cgtgcgccgc ctgtacctgc tcgcgtactt
    10681 tttccagact gcccgtgagc gtgggtttga taactaccgc gcgcacgccc tcttcagcca
    10741 caaaggcaaa atccggcccg cgcaggcttt catcccaggc aatggcaatg ccggtttcac
    10801 gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt
    10861 cgcgataatc cgggttaacg tatttggcaa actgctgacc tttcagcggt gtccaggcgc
    10921 gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc cagcaacaga ttcaccacca
    10981 tgccgtcgcg caccgcttcg tacaatccca ctttgacctt cgccactttc tcgcccggca
    11041 tatcggcaag tttgaggatc agatcgtccg gatcgccatt acacagcggt gccgcacggt
    11101 agttggctgc ttgcggcaac gtatctgtca gttctgccaa tgcacagctt acgccaaagg
    11161 ccacggaagg catctgcggt agctcgcaat cgcctgccag ccagttattt acccaggcaa
    11221 gcagcacact ttgcgcctct tcccaggttt cctgactgaa gcccggcagt ggggagatct
    11281 ccccccaccc ttcgcgctcg ccttcacgca ggcaaacata cagcccgtcg cgggttttta
    11341 accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg tatacctgcg
    11401 cgctacgcat tacggattcc gtttgaattt gctgaagtca ggctgacgtt tctggttgaa
    11461 ggcgttgcga ccttcctgac cttcttccgt catgtagaac agcatggtgg cgttgcccgc
    11521 cagctcctgc agccccgcct gcccgtcaca gtcggcgttc agcgcagctt tcaggcagcg
    11581 cagcgccatc gggctgtttt gcagcatttc gcggcaccaa cggacggttt ctttttccag
    11641 atccgccagc ggtaccacgg tgttgacaag gcccatatcc agcgcctgtt ttgcgtcgta
    11701 ctgacggcac aggaaccaga tttcacgcgc ttttttctgc ccgacgatac gagccatgta
    11761 ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac cgaagatggc
    11821 attatctgcc gcgatagtca ggtcgcacat catgtgcaga acgtgaccac cgccgatgga
    11881 gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac gctggaagtc
    11941 cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat caccacgcac
    12001 tttctggtca ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa tgatcacgcc
    12061 gacgttatcg tcataacgcg catctgccag tgcctggatc atctctttta ccgtcagagg
    12121 acggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac cgtcggtgga
    12181 tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg tgccattcaa ccggtgcgta
    12241 aagcattgct tcatcaggat aaatcatatt gtgtccttta gtcagaaacg caagatctgc
    12301 gccagacttg cgattacgcc agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga
    12361 atgacatggc agtcggcagc cagttccgcc gccagggcgc ggaatttgct gtcacgttca
    12421 ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg
    12481 acggcgagag aagtcgcctc cagcatggcg gcaagcgttg cgccattatt gttgctgcgc
    12541 agcgccacca gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac
    12601 cagtcggcaa ataccgcggt taatggttcg gaacgaaaac gctgcgccca ttggcgatcg
    12661 gaacgctgac gttccgcgcg ttgttcagca ttttgcagcc ccggatgacc gccttcgaca
    12721 acaaccccac aaagccccgc cagcctctgg caagccgcca tcatcgccac ccgtccgcca
    12781 agcgagtacc ccaccagcca gaagttaagg atgttgtaac taaccagggt tttacgcagt
    12841 aagtcggtga catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg
    12901 agatcaacat acaaccgtga gtagtcggca aacacctcgc ccacttcttg ccattcgtgg
    12961 caatcgccgg aaaaaccgtg caaaaacacc agccagggta aacctggttt gccgtgtttt
    13021 gcctgcgcgt gcaggatcat aaatggctta cctgcgccag aagttgctgg agcgtttgcg
    13081 cgccgtcggt gtcgttaacc accatttcaa ttaccgtggt ggttggcgtg cgccaggcat
    13141 cggcaagtgc cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca
    13201 tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcgcgctcgc
    13261 ttttcggcgt tggcagcagc gagaaaattt gcccgccgtt gttgttcacc acaattaata
    13321 ccagcggcgc ggaaacctgg cgcaataacg ccagcgcatt caggtcgtaa agtgcggaga
    13381 gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg ctgaacgccg gcggcagtcg
    13441 agagcagccc gtcgataccg ctggccccac ggttgctgta caccgggtaa cctgccggaa
    13501 gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt accgacaaac aattgcccct
    13561 gttcaggcag atagtcgcag acgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac
    13621 gggcaataac cgcctgcatc gcctgctccg ccaggcgcgg gatttcaacg caccagggct
    13681 ggcgtttttc tgccggatgc agttccagcc agtcggcaat attggcaatt aaccgacgcc
    13741 cgcgatggtg cgccggatca agccgccctt cgatgtcatc cacaatccag tactcttcgg
    13801 gttcacagct tgcctgccat tgcaggagcc gtttgccagt caggctgctt cccagttgca
    13861 ccacaatttg cgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg
    13921 cacacggcag cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag
    13981 tttgcgccca cagtgcaact tttttgccct cttccgcact catgcgcccg gcaaccacca
    14041 cgccgcgttt ttgccgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg
    14101 cttcacgcag ccacggctta tcgtcctgcc accagtcgcc cagacgctgt tgccagctaa
    14161 gcccggtatc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc
    14221 cacccgcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa cgtgcgggga
    14281 tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg gtgagaggcg aacattcccg
    14341 gctggcgaat tgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta
    14401 agagaatcag tttttctccg gttaacccgg cttcaatcag tgccggatag agatttgcca
    14461 ccgccgtacc ggaggtgaca atcaccgcca ccggctgctt gctgactttc gccagcccca
    14521 gcgccagatg ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct
    14581 ccgccgccgc taacgttaac ggtgtagaac gcgagccagg ggcgatacag atgtgtctta
    14641 cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta
    14701 ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga
    14761 ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc
    14821 agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga gccacggaca
    14881 atgcccgcgc cagcatatag ccgcacgaca ttgccgctga tttttgctga gcgcagggaa
    14941 acacagaatt cgctttgctg taatgagaga tagcccgcag aaccggcgta ccattctcgg
    15001 gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact
    15061 gctgccgttg gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgaagtc
    15121 cagatacagc ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca
    15181 tccagcgtct gggtatcggc ctgtaatcgt tggcagatgt cttccaccac cagcatgttc
    15241 tcgcgctggt ttttatcatc cgccatcagc cactctccta actgctgcgc ctgcttatca
    15301 tcaggatgat ttgctactgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc
    15361 cgccataacc gttccggtga agagccaaga aaagcatttt cgccatcaaa ggccatgtaa
    15421 aaatggtagc aattgagatt cagtcgacga ctggccgcca tcatcgccgc cgcattgacc
    15481 ggacttgcga aatgcaggtc agttgcccga gcaaacacca ctttgtcgag ctcaccttcg
    15541 gcgatggttt tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt
    15601 tgttctcgcg tagtggttaa atgtaaccca ggcaagggct tgatactcac cagtgtggcg
    15661 ataaattctc ttgccttaat cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcagc
    15721 cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc gtggtaaaag taaattgccc
    15781 tgcgacgggt caaacgcatt cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg
    15841 cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca
    15901 gcttcttcat caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc
    15961 agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcgat aacccgaatg
    16021 ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta
    16081 agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg tactacaaaa
    16141 aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc
    16201 agcccaacgg ccgcacccac accaattccc tgccagggtt tttcgtggac gtagtcatca
    16261 gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt
    16321 tttttcacat catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg
    16381 gcgggatcgc cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtcg
    16441 tcatcgatac gtgtatcacc aaactgatta gacattctcg ttctccattt gcgtaaaacc
    16501 tgtcactaac tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca
    16561 atgtgtggga taccatcttc ttcatagatc tccgtcacag ggataaaacc gaaactctgg
    16621 tagaagtttt gcagatgcgc ctgcgccccg aggtagacag gcttatcagg ccagtgatgc
    16681 gtgcaagtgt cgagggtttt actcatcagt tgctgcccca ctttttcgcc acgcaacgct
    16741 tcactgacaa tcacccgacc tataacgacc ggctcaagat catcatcact tttcagaatc
    16801 ctcgcatacg ccaccagttc atcatttttc caaccgagaa tatggcgatt atcccctgtc
    16861 aaatcatcac catcgatatc ctgataaggg caattctgct cgacaacaaa caccgcgcaa
    16921 cgcagttgta ataaggcata aagctgggaa accgataatt cagagtgatg cagatcttgc
    16981 cattcaatca taatgctctc cttttagatg tatgccttat actagccgga atattttttg
    17041 aaacgtgatg aactcatgga attaattttt ttaggtactt cggccggtgt gccaacccgt
    17101 acgcgcaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag cggtctttgg
    17161 ttgtttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga
    17221 aaactggaca agatttttat cagtcacctt catggcgatc atctctttgg tttacccggc
    17281 ttgctgtgca gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcaa
    17341 ggtatccgtg aatttgtgga gaccgcgctg cggattagcg gctcatggac cgattatccg
    17401 ctggaaattg tcgaaattgg cgctggcgaa attctcgatg atggcctgcg caaagtgacc
    17461 gcttatccaa tggaacaccc actggaatgt tatggctatc gtattgaaga acatgataaa
    17521 ccgggtgcat taaatgccca ggcattaaaa gctgctggcg taccgcctgg cccactattt
    17581 caggaattaa aagcgggcaa aacaatcatg ctggaagatg gaaggcagat taacggcgca
    17641 gattacttag ccgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc
    17701 tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg
    17761 gatataacca tggaagccaa agccaatagt cgcggtcata gctctacacg ccaggctgcg
    17821 acactggccc gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat
    17881 gatgataaag gttgtcagca cctgttacgt gaatgcaggt caattttccc ggcgactgaa
    17941 ctggcgaatg atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga
    18001 aacttgccct gttatcgcaa cccgcgctac atatatccac ccacacgggc atattgagcg
    18061 atttttgata tttattcatc tccttacgca ctatattcag agcaataagt tgcctctttt
    18121 tatcttcatt cagcactacc tttgcaacta aaaggagcat atgatggtta cagttgtcag
    18181 caattattgt caattatctc aaacccaact cagtcagaca tttgcagaaa aatttactgt
    18241 gaccgaggaa ttactacagt ctttaaaaaa aacagcgtta tccggagatg aagaaagtat
    18301 tgagttactg cataacattg cgttaggtta tgatgaattt ggaaaaaaag ctgaagatat
    18361 tctttaccat attgttagaa acccaacaaa tgatacccta tcgattatca agcttataaa
    18421 aaatgcctgt ttaaaattat ataatttggc acataccgca accaaacacc ccctcaattc
    18481 acatgattca gataatctcc tgttcaaaaa actattctct ccttcgaaat taatgacaat
    18541 tatcggtgag gacattcctc tcatatcaga aaaacagtca ctttcaaagg tacttttaaa
    18601 tgataagaat aatgaactga gcgatgggac aaacttctgg gataaaaatc gtcaattaac
    18661 cacagatgaa atatcttgct atcttaaaaa gatcgccgcc aatgcaaaaa atactcaagt
    18721 caattatcct actgatttct acctccccaa ttccaacagc acttacctgg aagtcgctct
    18781 caatgataat attaagagcg atccatcatg gccgaaagaa gtccagttat tccccataaa
    18841 tactggcgga cactggatat tagtttcgct acagaaaata gttaatgaaa aaaacaacac
    18901 acaacaaata aaatgcatca tattcaattc attacgttca ctaggccatg aaaaagaaaa
    18961 ttcacttaag cgtatcatta acagtttcaa tagtttcaat tgtgacccca cgagagaaac
    19021 gc
//
LOCUS       DAZGCQ010000096        18108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000096 DAZGCQ010000000
VERSION     DAZGCQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.096"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(243..1835)
                     /gene="lsrK"
                     /locus_tag="ACHK07_003763"
     CDS             complement(243..1835)
                     /gene="lsrK"
                     /locus_tag="ACHK07_003763"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO8104045.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1914..2867)
                     /gene="lsrR"
                     /locus_tag="ACHK07_003764"
     CDS             complement(1914..2867)
                     /gene="lsrR"
                     /locus_tag="ACHK07_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO8104046.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3116..4651
                     /gene="lsrA"
                     /locus_tag="ACHK07_003765"
     CDS             3116..4651
                     /gene="lsrA"
                     /locus_tag="ACHK07_003765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO8104047.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAVGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4645..5673
                     /gene="lsrC"
                     /locus_tag="ACHK07_003766"
     CDS             4645..5673
                     /gene="lsrC"
                     /locus_tag="ACHK07_003766"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO8104048.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKTAS
                     SGKKREAA"
     gene            5673..6665
                     /gene="lsrD"
                     /locus_tag="ACHK07_003767"
     CDS             5673..6665
                     /gene="lsrD"
                     /locus_tag="ACHK07_003767"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO8104049.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLGLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6677..7699
                     /gene="lsrB"
                     /locus_tag="ACHK07_003768"
     CDS             6677..7699
                     /gene="lsrB"
                     /locus_tag="ACHK07_003768"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO8104050.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7726..8601
                     /gene="lsrF"
                     /locus_tag="ACHK07_003769"
     CDS             7726..8601
                     /gene="lsrF"
                     /locus_tag="ACHK07_003769"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO8104051.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8625..8915
                     /gene="lsrG"
                     /locus_tag="ACHK07_003770"
     CDS             8625..8915
                     /gene="lsrG"
                     /locus_tag="ACHK07_003770"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO8104052.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8972..9730
                     /gene="tam"
                     /locus_tag="ACHK07_003771"
     CDS             8972..9730
                     /gene="tam"
                     /locus_tag="ACHK07_003771"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO8104053.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9734..10648)
                     /locus_tag="ACHK07_003772"
     CDS             complement(9734..10648)
                     /locus_tag="ACHK07_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO8104054.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10845..12296)
                     /gene="uxaB"
                     /locus_tag="ACHK07_003773"
     CDS             complement(10845..12296)
                     /gene="uxaB"
                     /locus_tag="ACHK07_003773"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO8104055.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12523..13941)
                     /locus_tag="ACHK07_003774"
     CDS             complement(12523..13941)
                     /locus_tag="ACHK07_003774"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8104056.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14080..14439)
                     /locus_tag="ACHK07_003775"
     CDS             complement(14080..14439)
                     /locus_tag="ACHK07_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO8104057.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14439..15365)
                     /gene="glsB"
                     /locus_tag="ACHK07_003776"
     CDS             complement(14439..15365)
                     /gene="glsB"
                     /locus_tag="ACHK07_003776"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO8104058.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15429..16817)
                     /gene="sad"
                     /locus_tag="ACHK07_003777"
     CDS             complement(15429..16817)
                     /gene="sad"
                     /locus_tag="ACHK07_003777"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO8104059.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIAAAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16918..17799
                     /locus_tag="ACHK07_003778"
     CDS             16918..17799
                     /locus_tag="ACHK07_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8104060.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17877..>18108
                     /gene="yneK"
                     /locus_tag="ACHK07_003779"
     CDS             17877..>18108
                     /gene="yneK"
                     /locus_tag="ACHK07_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO8104061.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGN"
BASE COUNT         4535 a         4600 c         4514 g         4459 t
ORIGIN      
        1 aaatcgaagt ttattaaacc ccttttgcat ccattaatgt ctttaggaat attcgctata
       61 aaataagggt tatgcttaat gctttaagaa aaatagcaac tttcccttga atatagtact
      121 ggtgatggaa cgatgaatct gcaagctggc atttaacaag ccagctctaa aagaagggaa
      181 ataagaaaaa ctatactcaa aaactaacag ccacggtcat catgatgtgg ctgtcaatga
      241 aactataacc caggcgcttt ccataacgat gtcgtcagtc catgatcaac cagccccagc
      301 tgatcctgat aaactgcctg ccacttatcg cgtgaatcct gataaagttc atgcttttcc
      361 gggtctggtg tgtgcgtccg ttcccagcga accaggcgtt ctccggtttc tgccattgat
      421 gaaaaaattc cggcaccgac gccagctgca atggcacatc ctaatgcggt ggcttctttg
      481 accaccggaa tattgacggg taatcccgag acatcagcga gaatttgact ccataatttc
      541 ccttttgaac ctccgcctgc aaagactaac gatgaaggat gaatattcga gaaatcagca
      601 atttgctgca agttacacgc tgatacaatc gccgcatttt cttccagcgc acggaacaat
      661 gtcgctttgt tacatttatc cgggtcaatg gacaagttaa taaaggaagg cgcagcgtga
      721 taccaggttt taaagcgcat tctgtcggag aagatcggca ttacgcccca cgacccaggc
      781 ggcacccgac tggccatctc ttccagcagc gtataggtgt cgatgcctaa acgttcggca
      841 atcagttttt cttcggcaca gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa
      901 aagcttatag attcagcttg taccatgcca ggaataacat gaggattaac gcgcacgttc
      961 atttctgggt ctgtcaccgg cgcggctaaa tttacaactt gctgccagaa tgtgccgcca
     1021 agaaccgcgg tttgtgccgg acgcacaacg cctaacccca ggcaaccaag ctgcacgtcg
     1081 cctcctccaa cgaccaccgg agtgcccacc ttcagaccgc agagttccgc cgcttgtgaa
     1141 cttaccacgc ccagcaatgt gccggtttct ttgacaggag aaagaatatc ggcacgcagg
     1201 ccagccatat ccagcaatgc aggtttccag tcacggatgg ttagatcaag aagtcccgtg
     1261 gtgccagcgt tagagggatc caccgccagt tcgccgctga gcatataggc cagccagtcg
     1321 ctgatcatgg tgatggttga tgcctgacgg taaatatcgg aacgatggtg cgccagccaa
     1381 agtaatctgg ggatggcact taaagccagt gtttgtccgg tcgcgcgata aacttcgttt
     1441 tcaaaggtat tgttgtgcag ttctttaagt tcgctaactt cgcgcgctgc tctggcatcc
     1501 acattggcgc aggcccagat cggtgttcct tcattgttat ataaaacaat gccttcacgc
     1561 atagaacatg ccgaaacggc agcgatatac tccgggtcta tgccggcgtt gtgcagcgcc
     1621 tggcgcatac actcacacgc cagttgccag tttttgttga gatcaaattc catagaacca
     1681 ggaacgtccg gtactgccag atgccgccac tccgcctgtc ccactgctat ttgattgcct
     1741 tccaggtcga atatcacagc ccgaatactt ccggtgcctg catccagcgc catcaggtag
     1801 tactttgatt ctgaaggggt aaagagtcga gccattagat tatcctcggc ttatgcgttc
     1861 tgtctgttcc tctatacgtt ctccatcatt cccggtaata aggtcatgca aatttaacta
     1921 cgtaaaatcg ccgctgctgt gtcctgatcg gtaaccagtg cgttgatata accgcctttc
     1981 attgcagcgg caattgcttc ggctttattt tctccccctg ccacgccaac ccggacgggt
     2041 atggtcttca gcgcgcttaa aggtaagcca atcagttcgt tatgtatttt gatatccgtg
     2101 acaacgtcac cttttgcatc aaaaaagtag cctaaaatgt cgccaaccgc cccttttcgg
     2161 ccaatcatta actgttcgcc ctggctgata taaccggagc gaatgatcgt cgcgtcgtct
     2221 tgttgactca cggccccaat gccgacaatc gccacatccg ctgcttgcgc ggctaacaga
     2281 acatctttga cgcaattttc attttttagc gtacgggcaa tgtcagcgga ggatgcccgc
     2341 aacggagccg gaataatatt cacactgcac gccgcgttaa gctgcccgat acccgtcata
     2401 taggaaccga cgccaccgga gagcgtgacc aggcgaattt gctgtgacga aataaaacca
     2461 cttaagcgtt gcagcgtatt catggttgcc tcgccaaaac caatcgccag catctgttgt
     2521 ggttgaagta aactcatcaa catatgcgcc gcgcctatcc ccagtcgccc gccgacatca
     2581 gcatccgcaa gcccagggat cacccggaca tgttgcagcg aaaactgacg acgtaattgt
     2641 gtttcatatt ccagacagcc ttcaaagcga gaattaatct gcacgcgaat gatgccggac
     2701 tgatgccctt tctccagtaa tcgcgacact ttcaaacggg tcaggccgag acgatcgctg
     2761 atctcactct gggtcagccc gtcgtggtag taaaaccacg cgatccgcgc gacctgttct
     2821 tcttcacaca ttccctgttc tgaaattgcc gaatcgttga ttgtcataat ttattcttca
     2881 ctttgaacat atttaaatct ttaatgcaat tgttcagttc ttgcgcattt atatctgtga
     2941 tggcaaccac agtttgactc tacgagcatg aacaaacgca accgtgaaaa tcaaaatagc
     3001 ataaattgtg atctattcgt cggaaatatg tgcaatgtcc acctaaggtt atgaacaaat
     3061 taaaagcaga aatacatttg ttcaaaactc acctgcaaaa ctgaacgggg gaaatatgca
     3121 aacgagtgat acccgcgcgt taccgctact ttgcgcccgc tcggtttata aacagtattc
     3181 aggggttaat gtcctgaaag gcatcgattt tacgttgcac cagggggagg tccacgccct
     3241 gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg gtattacccc
     3301 tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa cgccagttca
     3361 tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc caagcctgtc
     3421 gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc agaaaatgaa
     3481 gaacttgctg gcggcgctgg gctgccagtt tgatctgcat agtctggcag gatcgctgga
     3541 tgtcgccgat cgccaaatgg tggaaatcct ccgcgggctg atgcgcgact cgcggattct
     3601 gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct tgtttagtcg
     3661 cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata agctgccgga
     3721 aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg ccttaagcgg
     3781 caaaaccagc gaactgtcta ccgacgacat tattcaggcc atcacgccag tggtacggga
     3841 aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggtaacc gcccacaaca
     3901 tgccgtagga acgtcggtgc tgacactgga aaatctgact ggcgaaggtt ttaggaatgt
     3961 cagcctgacg ctaaatgctg gagaaattct gggtctggct gggctggtgg gtgccggacg
     4021 cacagaactg gccgagacgc tctatggtct acgtactttg cgtggcggac gcattatgct
     4081 gaatgataaa gagatcaata gattctccac tggagaacgt ttactgcgcg gtctcgttta
     4141 tctgccggaa gatcgccagt cctccggact gaatctcgat gcttcgctgg cctggaacgt
     4201 ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag ataatgccac
     4261 cctggaacga tatcgtcggg cgctgaatat taaatttaac caaccggaac aagctgcacg
     4321 gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg aagcctcgcc
     4381 gcaagtattg atagtcgatg agccgacgcg cggcgtggat gtctcggccc gtaatgatat
     4441 ctaccagctg ttacgcagca tcgccgcaca aaatgtggct gtgctgctta tctcctccga
     4501 cctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg gcgaaattac
     4561 ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg ttgccttcgg
     4621 cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg tgaaatcacg
     4681 gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg ccagtattta
     4741 agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct gctggcaatg
     4801 ggcgcgacgc tggtaatgct tacgcgcaat attgatgttt cagtgggttc gattaccgga
     4861 atgtgcgcgg tgctgttggg gatgttactg aacgcggggt attcactacc tgttgcttgt
     4921 gtcgcgactt tactgcttgg tttgctcgca ggatttttca acggtgtcct ggtcgcgtgg
     4981 ctaaaaatcc ctgccattgt tgccaccctt ggcacgttag ggttgtacag aggcatcatg
     5041 ttgctgtgga ctggcggtaa atggattgag gggttacccg ccgaactgaa acagctctcc
     5101 gccccgctgc tgtttggcgt ttccgcgatt ggttggttga cgataattct ggtggcattt
     5161 atggcctggc tgttggcaaa gacggcgttt ggacgcagtt tttatgccac gggcgataat
     5221 ttacagggcg ctcgtcaact gggcgttcgt acggaagcca ttcgcattgt ggcattttcg
     5281 ttgaacggct gcatggcggc actggcggga attgtgtttg cttcgcagat tggttttatc
     5341 cccaaccaga ccggtaccgg gctggaaatg aaagcaattg cagcctgcgt gctgggcggt
     5401 attagtttgc tcggcggttc cggtgcgatc atcggtgcgg tactcggcgc atggttcctg
     5461 acgcagatcg atagcgtact ggtgctgttg cgcattccgg catggtggaa tgattttatc
     5521 gcgggtctgg ttctgctggc ggtgctggtg tttgatggac gcctgcgttg tgcgctggaa
     5581 cgtaatctgc ggcggcaaaa atatgcccgc tttatgacgc caccgccatc cgttaaaact
     5641 gcttcgtcag gtaaaaaacg ggaggctgca taatgcgtat tcgctacggt tgggaactgg
     5701 ctcttgccgc actgctcgtt attgagattg tctcatttgg tgcaattaac ccgcggatgt
     5761 tagatctcaa tatgttgctg ttcagcacca gtgactttat ctgcattggc attgtcgccc
     5821 taccgctgac gatggtgatt gtcagtggcg ggatcgatat ttcgtttggt tcgaccatcg
     5881 gcctctgcgc cattgcattg ggcgtactgt ttcaaagtgg tgtgccgatg ccgctggcga
     5941 ttctcctggg cttactgctc ggcgcattgt gcgggctgat caacgccgga ttaattatct
     6001 ataccaaagt taacccgctg gtgattacgc ttggcacgct gtatctgttt gccggaagtg
     6061 ccctgctgct ttccgggatg gccggagcga cggggtacga aggtattggt ggattcccga
     6121 tggcgtttac agatttcgct aacctggatg tgcttggact ccccgttccg ctgattatct
     6181 ttctgatatg tctcctcgtt ttctggctct ggctgcataa aacccatgcc ggacgtaacg
     6241 tgtttttgat tgggcaaagc ccgcgcgtgg cgctttatag cgcgattcca gttaaccgca
     6301 ccttatgtgc gctctatgcc atgacggggc tggcgtctgc ggtcgccgct gtgctgctgg
     6361 tgtcgtattt tggttcagca cgttccgatc tcggtgcgtc gtttctgatg cccgccatca
     6421 ccgccgtggt gcttggcggg gccaatattt atggtggttc cggttccatt atcggcaccg
     6481 ccattgcggt tttattagtg ggatatttgc aacaaggttt gcaaatggca ggagtgccaa
     6541 atcaggtgtc cagcgccctt tccggtgcgc tacttatcgt cgttgtcgta ggtcgttccg
     6601 ttagcctgca tcgccagcaa attaaagagt ggctggcgcg tcgggccaat aacccattgc
     6661 cataaaggat atcttcatga cacttcatcg ctttaagaaa atcgccttac ttagcgctct
     6721 tggcattgcc gcaatctcta tgaatgtgca ggccgcagag cgtattgcat ttattcccaa
     6781 actggttggc gtgggatttt ttaccagcgg tggcaacggc gcacaacaag cgggtaaaga
     6841 gctgggcgtt gatgtgacct acgacgggcc gacagaaccc agtgtttctg gtcaggtaca
     6901 gttgattaat aacttcgtca atcagggtta taacgccatt atcgtttctg cggtttcgcc
     6961 tgatggcttg tgtccggcac tgaaacgcgc catgcaacgt ggtgtgagag tgctgacctg
     7021 ggactctgat actaaaccgg agtgccgctc ttactacatt aatcagggaa cgcccgccca
     7081 gttgggaggt atgttggtgg atatggcggc gcgtcaggtg aataaagaca aagccaaagt
     7141 cgcgtttttc tactcaagcc ccaccgttac ggaccaaaac cagtgggtga aagaagcgaa
     7201 agcgaaaatc gccaaagagc atcctggctg ggaaattgtc actacgcagt ttggctataa
     7261 cgatgccact aaatcattac aaaccgcaga aggaatatta aaagcgtata gcgatctcga
     7321 cgccattatc gcccccgatg ccaacgccct gcccgctgcc gcacaagccg cagaaaacct
     7381 gaaaaatgac aaagttgcga ttgtcggatt cagtacgcca aacgtgatgc gtccatatgt
     7441 ggaacgcggc acggtgaaag aatttggcct gtgggatgtg gttcagcaag gcaaaatttc
     7501 agtttatgtc gcggatgcat tattgaaaaa aggatcaatg aaaacgggcg acaagctgga
     7561 tattcagggc gtaggtcagg tggaagtctc gccaaacagc gtccagggct atgactacga
     7621 agcggatggt aatggcatcg tactgttacc ggagcgcgtg atattcaaca aagagaatat
     7681 cggcaaatac gatttctgat gtgcattact taaccggagt aagttatggc agatttagac
     7741 gatattaaag atggtaaaga ttttcgtacc gatcaaccgc aacaaaatat cccttttacc
     7801 ctgaaaggtt gcggtgcgct ggattgggga atgcagtcac gcttatcgcg gatatttaat
     7861 ccgaaaacgg gtaaaaccgt gatgctggct tttgaccatg gttattttca gggaccgact
     7921 accggacttg aacgcattga tataaatatc gccccgctgt ttgaacatgc cgatgtatta
     7981 atgtgtacgc gcggtatttt gcgcagcgta gttcccccgg cgaccaataa gccggtggta
     8041 ctgcgggcgt caggtgcgaa ctctatcctg gcggaattaa gcaatgaagc cgtggcgtta
     8101 tcgatggatg acgccgtgcg cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc
     8161 agcgaatatg aacatcagtc gatcaaaaat attattcagc tggttgatgc cggaatgaaa
     8221 gtgggaatgc cgaccatggc cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt
     8281 tatttctcgc tcgcgactcg aatcgccgct gaaatggggg cgcaaattat caaaacctat
     8341 tatgtcgaaa aaggttttga acggattgtt gccggatgtc cggtacccat tgttattgct
     8401 ggcggtaaaa aattaccgga gcgcgaggcg ctggaaatgt gctggcaggc tatcgatcag
     8461 ggcgcttctg gtgtggatat ggggcgtaat attttccagt ctgaccatcc ggtggcgatg
     8521 atgaaagccg tacaggcggt ggttcaccat aacgaaacgg ctgatcgggc atatgaactc
     8581 tatctgagtg aaaaacagta actgcggatt taaggagaag aattatgcac gtcacactgg
     8641 ttgaaattaa cgttcatgaa gacaaggttg acgagtttat cgaagttttt cgccagaacc
     8701 acctgggctc tgtacaggaa gaaggcaatt tgcgcttcga tgtcttacag gacccggaag
     8761 tgaattcgcg cttttatatc tacgaagcct ataaagatga agacgcagtg gcgttccata
     8821 aaaccacgcc ccactacaaa acctgtgtcg cgaaactgga atctttaatg accgggccgc
     8881 gtaaaaaacg tctgttcaat gggttgatgc cgtgaggcga atttatcagt tttatctaca
     8941 attggggtaa cgcgctgacg ggagtaaata aatgtctgac tggaacccct ctttatatct
     9001 gcactttgcc gctgaacgat cgcgtccggc ggtggagctg cttgccagag tgccgctgga
     9061 gaatatcgaa tatgttgccg atcttggctg tggcccaggt aacagcaccg ccctgctgca
     9121 tcaacgttgg cctgctgcca ggataacggg catcgactcg tctccggcga tgattgctga
     9181 agcgcgcagt gctttgccag actgccagtt cgtggaagca gacatccgca actggcaacc
     9241 ggaacaggca ctcgatctga tttttgctaa tgcctcactg caatggctgc ccgaccacta
     9301 cgaattgttt cctcacttgg tttctttact taatccgcag ggcgtgctgg cagtacagat
     9361 gccagataac tggctggagc cgacccatgt gctcatgcgc gaagttgcct gggaacaaaa
     9421 ctacccggat cgcgggcgtg agccgttggc tggcgttcat gcttactacg atattttgag
     9481 cgaagccgga tgtgaggtcg atatctggcg aaccacttac tatcaccaga tgccgtcgca
     9541 ccaggcgatt atcgattggg taacagccac tggattacgt ccgtggttac aggatctgac
     9601 cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag atgttggaag agcagtatcc
     9661 actgcaagag aacggacaga tactgctggc atttccgcgt ctgtttattg ttgcccgccg
     9721 tacggagtaa atatcacgtc agttggtaat ggcgatcggg aagaattttt gctggaattt
     9781 cggcttcatc gttcatctgt aagaggtcaa tttcaatagc gttgcagatg gcatccagcg
     9841 gtaaatcatt gttttcagta ccgaacggat cttccagttc ttccgccaga caatccagcg
     9901 aaataaaagt ataggaaatc agcacagaga taaaaggcgt catgtaatgc aggtccacga
     9961 ccagcgcgaa cggcagcatg atacaaaaca gataaacggt acgatgcaaa atcagggtgt
    10021 aggcaaaggg aattggcgtg taggcaatgc gctcgcagcc tgccagaacc gctgaaatat
    10081 cgttaagccg atcgttgagg ctaataaaca ggatatctga aagttgtcca ttgcgtcgct
    10141 gaaccgccaa ccactccccc attattaaca agatgcggtt agctggagag tttgaagcca
    10201 gtacacgctg aagatcttca gtcttgagat aatgagccag cacttccgcc tgtggctgtt
    10261 tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg cagccgggca aactccctta
    10321 cacttgccga atctggcaat gtcgttttta cctcgcgcag taacgaccgt gaggcaatca
    10381 tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc gtaaccggca ttattacgaa
    10441 aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa cggtgcgagg gtgaatttga
    10501 tgcccagatg cgtgtaccag ggcagcatga aaataacagc gatagaaaag agaaaattga
    10561 gtagtaagcg cgaggatatc ttggataata ctgagccatg ccagacaaaa atacggcgca
    10621 gccagtgttg ttgtggacga acaatcatgg ttatcttcag gcgtggaaaa gtcgccctat
    10681 taaacgtgat tacgatcaca ttctcaagac acgacttaca aattacctac aagcttacaa
    10741 cattaacagg cgctgcatgt ggcacctgtt aatgataaag gtatatagga tgttgtaact
    10801 aatatagtaa aataagaaac ccggtcgaaa ccgggttcag agtattagca caacgggcgt
    10861 actgcttcgc gcatcccttt ttcgagaatc gcatccaggt cattagccac ctgctcgacc
    10921 agaccaggca cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct
    10981 accagttcct gggtaccgat aacacggtca cgatgctgac tccagagttg ctgataacgt
    11041 tccagccagt gagcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag
    11101 aacgcaatca atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc
    11161 tgccctgcca gtagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg
    11221 atagacagca gctgatgctt aatgtacggg ttacggaaac gcccggtgac tgcactggcg
    11281 aaagactcca gttcatcgcg aggcaaatcc agaaccggaa taatttcttc gtagatggct
    11341 ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt atccagccct
    11401 gcctggaacg ccaccggcac caacgcggtg tgtgcaccgt tgagtattgc cactttgcgt
    11461 tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt gtccagacgc
    11521 agctcggtcg ctaaggattt cggtccctga atcacaaaca ggtaaaagtg ttcagcggtg
    11581 tcgaggaaac catcgtgata acccagttct tcttccagtt tagctacttc atcacgcgga
    11641 taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg atccagccat
    11701 tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag ttcacgcaac
    11761 gcgtcgccat tgtagtcgat caactcacac gggatgatga tccaaccttt atccagcgca
    11821 ccgttgaaat ggctaaaacg ttcgaacagc agacgggtca gttttgccgg ataacttacc
    11881 gctggcgcgt catcgaattt atcgcccgca tggtagctga tacccgcttc ggtggtgtta
    11941 gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc gtattcactg
    12001 tagacgctga tttcacgatt aacagagcga atcagacgcg cgtcgctgac cgcttccccc
    12061 ttctcattca ggccacggat aatggtggtg tacagaccat cctgcgtgct cagtgacggc
    12121 gggaatgaag tttcaatcgg acgaacaacg accacaccag aattcagatc ggtgtgctca
    12181 ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc accaaactga
    12241 atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt tttcacaatg
    12301 ggttcccttc tgattagtca tacaacctgt ttgaattggt acgacaggtt agcaaacttt
    12361 aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt ttatgagaaa
    12421 agtgtggcgc ggatcatggt ttaatcgagg aaaaacgcct tttcctggat cataaagtgg
    12481 tagaacacat tgcatttaaa tcgctctgaa tgaacaaaga tgtcagacaa cttcctcacc
    12541 gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca gacacgtatc
    12601 ggcttcgacc atcaatttat taaagtcatc ggtcagcgtt cggtacgatg cgcgaccact
    12661 accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag
    12721 ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat tcaccgacgg
    12781 cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat caccgacaat
    12841 cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat gtccatagtt
    12901 atcgttaata cttttgaaat agtcgatatc aagcagcatg acagtcagat gttgtgtctg
    12961 tttcagcgat ggacttttca gcgcctcata aagaccggac cgtgagtaaa cctgagtcag
    13021 aaaatcaaag tcggctcgca gcgcaacttg cttcattagc gaattgatcg ctgccacgct
    13081 aaaagaaacc ataattgggc atatcgccat cgtggcaata ccgagacgtg cggagaacat
    13141 ttccggaatg gagaacggcg aaccgaccga gatatcaatc accgaattag ccaccagtac
    13201 gatttccacc gcaccggtga caaaggtcaa cagacatgtt acctgcggcg tatagcgcac
    13261 tgcacaccag attaatgcag gcagcgggaa cgccagactc cccgctccgc caatgactac
    13321 cgaggcaata accgacacaa taagcgcgat ggcgggcatc atctgttctg ctttaaagcg
    13381 cggcagcact ccaggaatag ccaacgtcag catacaaggc acgatcaaca cgcccgttga
    13441 gaattgctca ctgaaccagt ccgcaagcaa aggccagaaa tccagactat caatactgac
    13501 cgaaccaatc gccccgacaa tagcgcataa taatgcggca atcagacagt aattaaatag
    13561 ccgtaaggca cttactggct catatttatt tttccccaaa cgcttatccc gcacgaccag
    13621 taaggcgacg gtaacaataa acatcatatt ggagaaattg atagccagtg aaaccagtcc
    13681 ccattcggtg gttatggcat cataaaccag catcgccaca taactgatcg catagtaatg
    13741 cagtcgatta agccagacat agcgggcaaa cacccccgcc attacgccat tcagcggcca
    13801 gaaaagagaa agttcgtgaa ccagccgtaa ctctgcacca ataaaataga acagcgtggt
    13861 tagcgcaaaa atagcgatgc tattacgtag cagatgacct tcctggaaca cacggaaagt
    13921 tgaaatgggg tgtacatgca ttaattatct gtccatacaa ttgcgggtgg caattatctc
    13981 aacgtaataa gtaaattctc ataaaggata gcatgttcgc tgacagcggc ggcaaataaa
    14041 ggtaccctgg gaatgctatt ttgtcgtttt ccgcaattct catggctgat tcatctgtac
    14101 aaccagccag tgatagatta ccgccacgat ataacgttgt tgttcttcgc ttagcgaaac
    14161 accctgcgga atgttatgcc acttagccag acaaccgcga cagcaggtcg cggtggcgtg
    14221 ctgagcgata aacaccggat gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa
    14281 tgccggagca agacgtttag cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc
    14341 tttctccaga caatactgac gctctttcat gcccaaacga aagcgagatc gaaattttga
    14401 acgggataaa cgcgcgaaga gtggatcgag agactgcatt aataaaccga acgccctaac
    14461 tgtttcgtca attgttcaag gacggcgata cccgcaagcg agttacctgc atcatccagt
    14521 tccggactcc agactgcgat ggccatttca tgcggaacaa tcgccacaat accgccacca
    14581 acgccagatt tcgccggtag tcccacccgc caggcaaact cccccgcgtt ctggtacata
    14641 ccactggtcg ccatcagcgc gttaatttgc cgtgcctgca ttggcgtcac cacaggttca
    14701 tcaatatgaa tagctttccc ctgattagcc agaaagacaa acgtccgggc cagctctaca
    14761 cagctcattt tcagagcgca gtaatgaaag tagttttgca gaacggttgt cacgtcatga
    14821 tggaaattgc caaacgactt catcagccag gcgatagccg catttcgcgc ggaatgttca
    14881 aattcggaac gcgctaccac cgtatcgtag gaaatatcag acacaccgct taagccgcgc
    14941 acgacttcca gcatacgttg ccgtggtgcg cttaatcgcc cttgcaacat atcgcagacc
    15001 accagcgcac cggcattaat aaacggatta cgcggaatac cctgctccat ttccagttgc
    15061 actaaagaat tgaacggtga tccagacgga tctttgccga cgcgttgcca gatttcctct
    15121 tcggagtaat gacgcatggc gacaacgaga ctcagcactt tggaaataga ctgaatggaa
    15181 aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca gatagcaatc
    15241 cccaatcggg aaccgtctac tgtagccagc gccggaatat aatccgcgac tttaccctga
    15301 ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc catggcgact
    15361 gccactttct actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctgaat
    15421 atgtcgcgtc agatccggtc tttccacacc gtctggatat tacagaattc gtgtaagccg
    15481 aaatgggaaa gctcacgacc aaagccactc tttttcacgc caccaaaggc cactcgcgca
    15541 tcgctggcac aataaccatt gataaacacc ccaccgcatt ccagacgtgc cgccatctgt
    15601 ctggcctgtg tttcgtcagt ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta
    15661 gccagttcca gtgcatgttc tgcatctttc gcaatggtga ttgccgcaac ggggccaaac
    15721 atttcttccc gaaacgcggt catttctggg gtaacattcg ccagaaccgt tggcggatag
    15781 tagttacctg ccccagccat cttttccccg cccagtaaca aacgcgcacc ctgcgccagg
    15841 gttttctcca cctgatgatg cagctcatca cgtaaatcga aacgagccat tggtccgaga
    15901 gcgttctctt cgtcacgggg atcgcccatt ttcaaggctg ccgcagctgc cacaaaacgt
    15961 tcggtaaatg ccgcagcaat tccctcttcg ataataaagc gttttgccgc tgcacaaacc
    16021 tgtccggtat tctgataacg tccggctacc gccgctttaa ccgccagttc cagatcggca
    16081 tcgttaagca caataaatgg atccgaaccg cccagttcca gtacgcattt tttcagtgcc
    16141 gctccagcct gtgcaccaat agccgctcca gcacgaacac ttccggtcac cgtgatagca
    16201 gcaatgcgcg agtctttaat catctgagtg acaccgtcat tgtcggcatt cagccagcca
    16261 tatacgcctt gtgggattcc cgcatcttta aacacctggg caatgagctg agcacagccc
    16321 atcacattcg gcgcatgttt aagtaagtaa ccgttaccag caagaatgat gggaacagcg
    16381 ccacgcatca cctgccataa cggaaaattc cacggcataa tcgccagaat cgtccccaac
    16441 ggtcgatact caataactgc ctgctgattt tccaccagcg taggttccgc cttcagcatt
    16501 gccggaccat gttctgcata ccagtcacac aaattcgccg atttcgccac ttcagcgcgc
    16561 gcctggttga ttggtttgcc catttcgcgg gtgatcattt gcgccatttc ttcgctacgg
    16621 gcgcgcagag ccttaccgat atcacgcagt ttttcagcac gataatctat atttgtctcg
    16681 cgccagtcac gaaagcctgc tgccgccagc tgaagtgcgt tttcgatatc atcagcgcca
    16741 gcccacggca acacagaaag ttgttcaccc gtggcaggat ttatcgaaat tgcatgagtt
    16801 gccggagtaa tggtcatcgg ggtatctcct ttatgagtca tggtatgaag atacgcagat
    16861 ttactcttgc tttaaaatga ataatattaa gccacttatt cacgaatcga gaatattatg
    16921 gatctgaccc aactggagat gttcaacgcg gttgccgaag ctggcagcat tacgcaggct
    16981 gcagcaatag tgcatcgcgt gccgtcgaat ctcactaccc gtctacgcca gctggaaaca
    17041 gaactggggg tagatctgtt tattcgcgag aatcagcgtt tacgtctatc tcctgccggg
    17101 cataactttt tacgctatag ccaacaaatt ctcgcgttag tggatgaagc gcggagcgtt
    17161 gtcgctggcg atgagccgca aggtttattt tctctgggtt cgctggaaag caccgcagca
    17221 gttcgcattc cggccacgct ggcggaattc aaccgtcgtt atcccaaaat tcagttttca
    17281 ctttccaccg gcccttccgg cactatgctg gaaggtgtgc tggagggaaa acttaatgcg
    17341 gcgtttattg atggacccat taaccatact gccattgacg ggataccggt ataccgcgag
    17401 gagctgatga tcgtcacgcc acaaggtcat gcgccagtaa tccgtgccag tcaggttaat
    17461 ggcagtaaca tttatgcctt ccgcgccaat tgttcgtatc gtcgccactt cgagagctgg
    17521 tttcatgctg acggtgccgc tccgggaact atccatgaga tggagtctta tcacggaatg
    17581 ttggcctgtg tggtcgcagg agcaggtatt gcgcttattc cgcgctctat gctggaaagt
    17641 atgccggggc atcaccaggt tgaagcgtgg ccgttagctg agcaatggcg ttggctaaca
    17701 acctggctgg tctggcgtcg tggtgcgaaa acgcgtccgc tcgaggcatt tattcaacta
    17761 ctggatgtgc ctgactcgac aaaacagggt taccaatgag ctatttttga tagttcttgc
    17821 ggctaatatg ctctatatag tgatgttccg atgacttatg actatatggg gcaaatatgg
    17881 ttacgccagt aagcatcagc aattacatat ctcttcctga tgattttccc gttcgtaata
    17941 ttgcaccgca ggtaaaagaa gttttaaagg attttattga tgcacttagt acaataatat
    18001 gtaatgaaga gtggcgcacg tctttaaaca tcaactcagc cacaaaaaag atatttaata
    18061 accttgacaa cttatcatat attcagagga cttcttttcg gggtaacg
//
LOCUS       DAZGCQ010000097        17790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000097 DAZGCQ010000000
VERSION     DAZGCQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.097"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..8584)
                     /locus_tag="ACHK07_003780"
     CDS             complement(197..8584)
                     /locus_tag="ACHK07_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO8104062.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKVTGKSVSDGAKASDEWLTGKM
                     SPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVAKKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETAG
                     MDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAAP
                     QPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQ
                     GETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRI
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVVRGVMSGIKDSD
                     LKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEI
                     IHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAH
                     MAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTP
                     REDGVGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVSRREMLRVGDTPRVL
                     RALGAPDLELVMPASVVHKATRPEIREHFVSVETLADLPQLLTDPVVVMRSKTESDAL
                     VSLIQATDTKGNPVIVAIHTHVKGDGFAEINKIASAYGKDKRSALQDQLDTSLLYLNK
                     EKAAEWLHAVGLQLPEANTINGSSGFKILHPEDIRKGPYYSRTSSLTPEDTIASRFVR
                     QMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKL
                     LADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGK
                     QAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLP
                     RTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNP
                     DKDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTY
                     YIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTA
                     VMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGTQWQKVWK
                     EFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVE
                     NALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTAN
                     LVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWY
                     DKVPSHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRA
                     AGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENM
                     PFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGG
                     TGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAE
                     LKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMV
                     KSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(8670..9932)
                     /locus_tag="ACHK07_003781"
     CDS             complement(8670..9932)
                     /locus_tag="ACHK07_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104063.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEAIGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKEASKVVAQAIKDGRDAQAANDEFLAAYDSL
                     PHQQKVQQRKTQQLLQKWQPWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(9943..10215)
                     /locus_tag="ACHK07_003782"
     CDS             complement(9943..10215)
                     /locus_tag="ACHK07_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO8104064.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            complement(10225..10671)
                     /locus_tag="ACHK07_003783"
     CDS             complement(10225..10671)
                     /locus_tag="ACHK07_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104065.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(10674..11324)
                     /locus_tag="ACHK07_003784"
     CDS             complement(10674..11324)
                     /locus_tag="ACHK07_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104066.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGGAKTPGTGANAIQHQAQNWRL"
     gene            complement(11418..11819)
                     /locus_tag="ACHK07_003785"
     CDS             complement(11418..11819)
                     /locus_tag="ACHK07_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104067.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(11876..12016)
                     /locus_tag="ACHK07_003786"
     CDS             complement(11876..12016)
                     /locus_tag="ACHK07_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8104068.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(12247..12981)
                     /locus_tag="ACHK07_003787"
     CDS             complement(12247..12981)
                     /locus_tag="ACHK07_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8104069.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHSRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(13072..13689)
                     /locus_tag="ACHK07_003788"
     CDS             complement(13072..13689)
                     /locus_tag="ACHK07_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104070.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(13695..13973)
                     /locus_tag="ACHK07_003789"
     CDS             complement(13695..13973)
                     /locus_tag="ACHK07_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104071.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(13988..15256)
                     /locus_tag="ACHK07_003790"
     CDS             complement(13988..15256)
                     /locus_tag="ACHK07_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8104072.1"
                     /translation="MSKKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(15253..16878)
                     /locus_tag="ACHK07_003791"
     CDS             complement(15253..16878)
                     /locus_tag="ACHK07_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104073.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSELPI"
     gene            complement(17416..>17790)
                     /locus_tag="ACHK07_003792"
     CDS             complement(17416..>17790)
                     /locus_tag="ACHK07_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8104074.1"
                     /translation="AEKARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLE
                     PSVAAAKAGKLPEAFFWTDAENNDVPVTAEEIIALSEAAEQAMFTKGMEIHVRQRTMK
                     KELEKLTSADEILAYRVGWAQE"
BASE COUNT         4012 a         5217 c         3934 g         4627 t
ORIGIN      
        1 ctcctgagac atagagcaac gtaggaaccg aaataagaaa aatcttaccc tcatcaggca
       61 tgacatttca catctgctta cactgtaatt tctgatcaag atcacaacat tatccgaatt
      121 taaatgttca gttcatctac aatatttccc tgcgtgactt tatatcagcc ccgtacatcc
      181 gtacggggca tgttctttac tccactccca cttttttgtt gtactcgcgg ttaaaccgat
      241 ccacggcaat tttcatatcc cgctcaaccg atttcaccat cgccgactgt tgcgccagac
      301 tgagagtact gtcggcgtaa atggcatcac gctgtttacg caaatccttc agtctcttcc
      361 gggtatcctg cataagcccg ttcatcgaca attttccgtt gttctcgtca atgaacgccg
      421 ttctttctgc accggtcaga ctcttcagct ctgcgtgata ctgcgcgacc tccgtcatcc
      481 ggtcgtacat cttctgctgg tcagcatacg gcatcacctc acctgaaatt ttccccagga
      541 agggcacctg ctgctccggt atatcaatac cattcagcga tttcaccgcc gcatccgtgg
      601 ttttagaaat gaagcgcccg gtcccgccag agatatagtc aatccagaat ttcagcgatt
      661 ccggtgtgat atccaccgcg ccgggacggt actggctgcc accagagaac gcattcagcc
      721 aggatgcaaa cgccttgtac gcttctggcg ttgaacgtct tcccagctgg ctgtcaggtt
      781 ttggtgtacc aaacggcatg ttctcctggt aaatctgcgc ccccatgaag ttttcattca
      841 tggcaaggtt cgcaaacgga cgcagaatgg tcggcgctgc atttttcagc aatgccccgg
      901 acagtgtttc cgacgtctca ctgccaatcg ggctgaatgc accaagcaca ccaccaacaa
      961 cattaccggc agcacgggac gccgtcaggt cacccgccgc cacaccttca gcggtatgcc
     1021 cgagcaggaa gaaaacgttg tacccgtaag gcagaggaat actccagtac tctccggcct
     1081 tgcccccgaa catcgatttc ataatgacga ggttacgctc tttcacatga gacggcacct
     1141 tgtcatacca gttaaccccg tcatcatcct cccccgcaac actgcggtta agcgagccaa
     1201 gcagataacc cgctcccaca gctgcaagcg cgattttctg cggtacattg agattcttcc
     1261 agcgaaggcg ctccagtaac ggcccgtcgc cattaagatg tccgagcgtt ctcaccagat
     1321 ttgccgttcc ctgaatactg gcgttggcga acatgtacag cgagttcatc agcgccccct
     1381 gctcaccacg acggttaaag ttcaccgtca tgtttttggc aagagacgcc gcctgctggc
     1441 gtgacaaacc ggcatcacgg gcgtgtttat aagctgacag acgcagtgcg ttttcaaccg
     1501 cgccgttggc atcctcgacc aggttcagga acgaattcca tgcaccgata ctctggcctt
     1561 tccatccccc cttcgccagt gagacaaggc gatccatttc cttctgctgg ccttcaaggt
     1621 cacccatgtt aaaccagccg gttttgcctc cgtcctcaac aaactctttc cacaccttct
     1681 gccactgtgt acctttgccc gtgagagttt tgcctcgcag actggcgtac acggctgaca
     1741 ttgcagaacg gctgtctttg actacagcca gggcagataa gttatccagc cctttcagtt
     1801 taccgtcgct ccttccctgc tctgccttca ggttcatcac cgccgtctgc acgtcacgga
     1861 tgaagttact gaccaggaat tccgggttat acgacgtgtt caccgttgcc aggaagcggt
     1921 taactttccc cagcgtacgg attacggcgt tactggtttc cggtcccata ttcttcatcg
     1981 cacgcatcag gcgcgggtca tggagtttga tgtagtacgt tttgccgttc tttttggtgg
     2041 tgaagtaccg gtctgccatc attgccatcg ccaccggacg ctcgacaact tcgcgaatgg
     2101 tttcaccagt ttcctggtcc ttgcgctctg caatcacacg catggtatcc ggtctgtcat
     2161 cagtgaatac ctgccagtaa tccttgtcgg gattatcctg caccagtttc aggaaggcgt
     2221 tacccacttc gtttttgcga tggcggatca gcgattcgct caggtcctgt atcgcctgag
     2281 tggacggaga ctgtgcacgg gatgcacgcc ccatggcctg cctgctttca cgtccgccga
     2341 tggtgaagcc cttacctgta cggggcagtg acaccacacc gtcaacatcc tggcctttca
     2401 ggggaacgta gtaacggtag gcgttctgcc aggcatccac cacaccgctc tcttccagtc
     2461 cggcctcacg gataagctca cggcgacggg ccagcatatc gtcaacaatc cctgccagac
     2521 ggtcatactg tgcctgtttg ccgctgttac gcacacgctg catgatttcc gccgcctccg
     2581 cgttggtcat ccccgaaccg ccgtccggca ttttcgggtt gattttcgcg atatgcgcgt
     2641 tacgttccgg cgcgtgacgg gcgtagaggt actcatccag atcggcctgc gcaatcttat
     2701 agtccgccag taatttagcc agcggctgaa catagcgctc cttcatcacg ttcaggtcgt
     2761 tttccgcctt cccgtggaag agttcttccg ccatataagc gttgttactg tcgtctattt
     2821 ttccgccagt tttacggata ttctcctgaa cagctttcag cacctggaat ttatcctgca
     2881 tctggcgcac aaaacgcgat gcaattgtat cctccggcgt cagactgctg gtacgggagt
     2941 aatacggccc cttgcgaata tcctcaggat gtagtatttt aaatccagag gagccgttga
     3001 tggtgttcgc ctcgggcaat tggagcccga ctgcatgaag ccattcagcg gctttttctt
     3061 tattaagata cagcaaagat gtatcaagct gatcctgcaa agcactcctt ttgtcctttc
     3121 cataggcact ggcaattttg tttatctctg caaatccatc gcctttcaca tgggtatgaa
     3181 ttgcaacaat gacaggattc ccttttgtat cggtggcctg aattaaagat accaacgcat
     3241 ccgattcagt tttcgaacgc ataactacta ccggatctgt cagaagttga ggcaaatcag
     3301 ccagtgtttc cactgacaca aagtgttctc tgatctctgg tctcgtagcc ttatgcacaa
     3361 cagatgcagg cataaccagt tcaagatctg gtgcccccaa tgcgcgcaaa acacgaggcg
     3421 tatccccaac tctgagcatt tcccttctgg aaaccgattg taatccggca atgcgacgca
     3481 cttctgaagc aaactgcttt gcatcatagg cgtcagcctt aaacggatca ggtttaccac
     3541 tacgggagta ctgagacgat gcacccactc catcctcacg cggtgtgtaa ccttcccgca
     3601 cacgctggcc taacgtacgg atggtctcgc gaacaagtct gatatcgttc agttccgtcg
     3661 gtttcagtaa ccccgtacga cgcagtaccc ctttgaccag ggcaacaaca cgctcccatg
     3721 ccgccacaaa tttattcggc tgtttctctg ccatatgcgc cagaaattca cccgcctgca
     3781 cttccggtga ttccttacca taggacgcat caaccttacg ccaggcttca cggatggtgg
     3841 cgttatcact gtcgcgggtt ttcagcacgg ttttgataat cgtctgatat tccgctggcg
     3901 tgacaacatg ctccatggca tggtggataa tctcgtgacg caacttctcg cgtacggtcc
     3961 gcccgtcagg gatgttatcc gccaccagga caatttctcg tttatccgga cgatagaatg
     4021 cgtgcaccct gccgtaacca tcgaacaatt cgcccgccag cgcttcagcc tctttctgtg
     4081 acttcaccac acggaccttc agatcactgt ccttaatgcc gctcatcacg ccacggacaa
     4141 ccgcttcaac ctgcgggacc gggctgcctt tggcttccgc actgcggtta acatccgaaa
     4201 tgagattgcc ttcaggtgtg cgggtcacgc ccttacggga ataaaacgca actcccttgt
     4261 ccgtctcacg ggttttcagc gtactgaaca ggtgatcgaa tgcatcacga ataccgccat
     4321 ccagttccgc attcgtcgga taagcccagg tgttatctgt gttgtgctca ggcgccttac
     4381 ggatattgac cagataatca ttctccacgc cagccatacg cgccttatcc tgaacataac
     4441 gctcaaaggc acgtgccgcc atttcaacat ccgttgacca gtacggtttt gagcgcacct
     4501 catcgagaag cgctgaacga cgcggcatgt cactgttttt aatggcctga atcacacctt
     4561 taaaagcgtc gtaaacctcc tgacgtaccg gatattcagc atcaacatac ctgccgtctt
     4621 taaatatgcg cctgacacgc tgtgcttccg tcataaagtc accacctgac gtaattttcc
     4681 cgtcattgga aacgtcataa cgaccaaaat aattatccag agaatggaac cattcgtgcg
     4741 ccagcgcacc cggtccgtta ccttttgtca ggttgattgc cacctcaccc ggctcatagt
     4801 gtgctgccgc cttaccctta ccacgggcac caaatgccag gccaagacga ccgttcaggg
     4861 aaagcgcttt tgtcggtaca ttcagtactt ccgcaaggtc atgcagcgag tcataagccc
     4921 ggttcaaatc agcctgacga cgcggacctt ccacataatt accaaactgc acaccacgga
     4981 aaccaaacgc atcactgaac tgctccggtg aaacattccc cttgcggcgt tctggtccgg
     5041 tacggtcgcg gttggtggcg ttgcgctgct cctcacgcga aatctcccgc atctccttca
     5101 catgacgaac aagctcatca cgatgtgaat caatgtactt acgcgcatca ctggctgact
     5161 taaagccacc tctcacccgc attttgtttt taccgtaagc gataaaaata tcgccactgc
     5221 gggtattccg gtaaacgtca aagcggattt tgtcatccgg tgacggtgct gttttttcat
     5281 cacctttcgc ctgtgatttt tcctcctgct ctgcaaacca gactttcgcc tttgtcagta
     5341 attcatccct gctttccgag aaaaagaggt tactaccctt attgtcctta ttgcgcagtg
     5401 aataaagttt ctgtggcgga tcgtaacgct tccctcctgc cgcctgatac acacccgata
     5461 ccacccgata agcagaagcc ctgtccatct gtgagggtgg cagagtgcgt aacagttgcc
     5521 aggtgtccgc gtaacgggag ggcattctgc cttccatcca ttctgcgagg cgtttcgcgc
     5581 tgaccgttcc gttcagcatt tccgatacac tgtgtcgtac tttttttatg ctctcccccc
     5641 agcctgccgt attgtgtttc gtcttcgccg gaatatcgct acgatacagc gctatcattg
     5701 ccagggtatc agcatcggca ccttcgttcg ccagttttgc gtagtccggt ttcgggaaca
     5761 gtttgctcag cggctgcgtg gcataatccc tgtcttccag cgttttaccc agtgtttcag
     5821 caagctgtgc ataacggtgt ttggctgccc ctttaatttc ctcgccaaag tcttcaattt
     5881 ttttgcccct gacttcacct ccgcgcacgg tagcttccct gtcagtagag acagttgttt
     5941 cagattcagg tgcgattgtc tcaggctgcg ggatgtcacg atttcctccg gcctgtcggc
     6001 gtacttctgc ctgctgtgca agctcaggca gtgagtcccg tacagtgcgg ggaagtgatt
     6061 caggctgagg catacgaaca ttacgcgcaa tgtcaggagc cggtagccct tcacgtaccg
     6121 gtgctgtcgc gttctgcgca tccggtgacg gcagaccacg acgaaccatt tcaccctcga
     6181 gtgtttcccc ctggcgacca gcggcgtttt ccggtgccgg agcttgtcct ttctggaaac
     6241 tctggccctt aacctcaccg gttgtggtaa aacgaccacc acgtcctgcc tgattctcat
     6301 ccggcgtacg cgccacctct cccggtaacg gatatccctg tccgggatga atatcgccgg
     6361 gagcgggcag gcgtggacgc tcagttaact cctgtgctgt cggaccggca tcaccttcag
     6421 ccatctgtga acgtacaagc tcctctgccg tcggcacgtt gccacgggca aggcgggcct
     6481 gtacctccgt atcatccccc gtgaaaccac ggaatcgtgg gtcacgcatg aacgcgggct
     6541 gctccatcgg gtcagcatca gcaatacgct ctccgtcagc cagggtgtta atggtttccg
     6601 ttgccacatc ttcagagaac gcaggattat ccatttcaac ggcatactga ttctcgcctt
     6661 tcttcactac ggacggtttc aggccggtgg cggcggcatt acggaaaaca tcactcccca
     6721 tggcgctttt ctcatccgtg aaataacggt tgtccgggcg caccttttca atgcgttccg
     6781 ctggtctgga cgcaccattc ccgttaattt caatgcggac ttcattcggg tcccggaacc
     6841 gcaccgtcgg gtaaatgctc ccgttaccat cctccgcatt ttcaggctgt gactgtgctt
     6901 ccggcgtcac ttcctgttgt gggcgaatga cttctcgtat tgcctgaaac tgttgttctt
     6961 cagtctcggt gcgcccctct ttctggctga gtctgcggta ttcctcaagc agttcagaac
     7021 gcggcttcgc cttcagctca ttcatcacag cctgtcgctt cgcctgttcg tccagttcat
     7081 tcaacaactg gctggcagct tcccggcgat gagctgcgga tgcgtcaccc tctgttgcca
     7141 tatccgcatc agcatactgc tccagaagct gctcgcgatt catgccctgc atggattcac
     7201 gctgctgcgc caccggatca acagattctg gttgcggggc tgcgtcgtcc tgctgcacgg
     7261 tttcagcatc acgcatggcg gtttcttctg cggcctgacg tctgccacga tatccggcaa
     7321 ccgcaccgga tggagccccc atcgcagcac caaaggctgc accttcgatc gttgcgtcag
     7381 ccacgccctc ccacggtgac acatccattc cggcggtctc acgcaatgcc gtgttttcct
     7441 gatagcgtga atagccgccc tgcgccgcat taatcgcccc ctgttccgtg gcatttctga
     7501 caatgccgct tttaacggtc ttcgctgtgc ctcgtgtcac cagattaaac agttgtgcgt
     7561 cacccagttt tgccgccatg gcattcacag ccagcaattc aggatcggtt gccagctgcg
     7621 cgcgcacctc atcggcaaca cgctcttttg ccagatccat tttctggcga tcagtaagct
     7681 gtgcgtgctg cgggtcggcg tcaatggaca aaaacgtctg ctgaaatttc ggtgactgcg
     7741 ccagctcaga gtaatccgca ttaagaacag catctgctgc cgccattgca ctctgcccct
     7801 gtgcactggc tgtggaatgg gtgatcaggc ccgcctggaa taaatccggc atttttttat
     7861 cgacagcttc tgctgccagt gccgtggctc tttccggctg catccctgcc gcgatgtatt
     7921 ttttctccag cccggcggtc agcatttttc gcagtgtgac atcacccacc tttttagcca
     7981 caccgccaac aaccatatca ggtacaagcg caccaatcag gtttacaccc ttcgccaccc
     8041 agaccgcaga atcatcatag ccttcggtca tcggcgtatt cagcgcacga acagcacccg
     8101 gcgacatctt accggtcagc cattcatccg aggctttagc accatcactg actgacttgc
     8161 ctgtgacctt cagaccttta ccgacagtat cagtcaccgc gtttttccct gttccatcgg
     8221 gaatgttttt aatcgcatct ccaacagaag gtggtgcgcc cagttccttt tgcgtggagg
     8281 aaggcggcat gtaaacatcc gtcatgaaag acgacaaccc ggcaagcgtt tttacaggat
     8341 tatttacaag tgcatcaccg agtccacggg ccatttcccc tgttccccgg acggactggg
     8401 cgaaaccttt acccattgtt ggcaatacat caccgaggct gaacgatgtg ctgtgatcaa
     8461 tggcattctg cgggttgctg aaaaaatcct gccagtaatt ttctcccggc tgcttaatgt
     8521 tcaggctgct gcggttctgg ttacccaatt gcgcttcagg gcgctgctca tctgaaaaag
     8581 ccatacagac tccagtaaaa aaaagcccga cacgatggtc gggcgtaagt gaacgatatt
     8641 aacggggagc gcgttactgc cgcatatctt tacttcatcc agcctttgat cttctcggca
     8701 gctgcggaag cgctataatc cagcgccttt tcatgctctg cctttttatc ccggaggtga
     8761 ttctggtaat aaatttccac ctccttttca cgaccaggtt ccagcaaagc aggcgcattg
     8821 gcggcagcct cttttacaaa ctcctgttta accggatcgc ccgccgccca cggctgccac
     8881 ttctgtaaaa gctgttgcgt tttccgttgc tgaactttct gttgatgcgg cagtgaatca
     8941 tacgctgcta aaaactcatc attcgcagcc tgagcatcac ggccatcctt gatcgcctga
     9001 gccactactt tgctggcttc cttctgaagc tctttcagag aaagtccctg ctgcttcggc
     9061 atgaaataac cataattttt cgatatctct gcaaatttgc tgcgatccct gacctgagcg
     9121 atagccttat caacgggtat cgccagcacg gtttgatcat cagggtgcgc actgccgtat
     9181 tctgttacag gtttatgcgc ggtggagcca tcgctgtatg tgagatcaag gccaataagt
     9241 acgtaccctt cctgctgcgc cggtacgata ctgccaatcc tggcctcttt tatcgttttt
     9301 tccccggttg aatcaggcat gccaatacgc tgttgtagtt ccggggcaaa cacgccggaa
     9361 agcacatcga gattttccgg ggtattcagc gaatcgatcg ccatgtccgg cttatcacca
     9421 aagatttttt gcaggttgat cacggcctga cccgctttcg ctgcatagcc ttcgtttgac
     9481 ataagaatta atggatgtcc ctttggcagc tgcccgaaca gacgcgttgc agcctcctga
     9541 tcgccagcct caattgcttt tccaatcgct tccattagtg gctggctatg ggcaatcata
     9601 tcgttatact caagccgtcg ctggttgtat ttctgcaact ggagtcgttg ctgttccatc
     9661 cccagcgacg cattccggta attctggttg gcgttaaact ccctttcctg cagtgtgtaa
     9721 ttgcggtcat caactttggc tctgtaatca aagttccgct gatcaacgtc tttattatgt
     9781 tcaaactgag actgcgcaaa ttcaaaatcg cgctcgttat ttttctgttg ctgagcaagc
     9841 tgtgcttctc gcaaaccaag ctccttacga cgggtcattg cctgatcaac agtgctgaat
     9901 ccagcaagta acccctgtgc aaatccgctc attcaccact ccttaaaaca aagcgcctgc
     9961 aatgccgcca attactgcac cagcaacagc gccgacggct gtccccacta ccggtactgc
    10021 tgaaccaaca gtcgccccga tagtggcccc cgtaccaatg cccgtaccga tattctgttt
    10081 tttctgagct ttctgttgag cagccagttg tttatttgat gcctcaatct ctgtacgtcg
    10141 tctgtctgcg tcacttattc cctgtaatgc ctcacgccgc gactgatttg caatatccag
    10201 taaaccgtat cccatattgc ccccttacgc tgccaccagt tgtccgccaa cactcagttt
    10261 ctgtcttgcc ggtgcagaag cccccgtcag aatattcatc tggcgatcct gttcggcttc
    10321 acggatacca tttttcgcgc cagcaattgc cagggcatta cgtaacccca gcgtattact
    10381 gtcgggatta tccgggcggt ttaccccgta tctcgccatc tggttatcct gcgccatctg
    10441 cgctgtacgg agactggaag tggcaaggcc tcccacccgt gcaagctgtg cattcatcag
    10501 actgttgttc tccccaaggt cagccagcct tgctacacgg ggtaaatatc tggtgcgcca
    10561 gtcgtcgtat tgctggcggg tcagcgctgc ggatgtctgc cagtccccct gagggcgggc
    10621 tgctccggta taaccgtatc ttgcaagtgt ttcgtattta ccgtactcca taatcacagt
    10681 ctccagttct gagcctgatg ctgaatggca ttagcgccgg tgccaggtgt tttagcaccg
    10741 cctccgctac cgccagcctt atgcatcgca tacgcacctg ccgcacccag accagcgcca
    10801 acaagagagg ctcgcccctg ctgtttcgta aacgccgcct gtgcatccga tttagctttt
    10861 gccagactgc tgtctgccag agagttaaaa ctctgtaacg catccgcctt ctgaccagaa
    10921 ccgagagcag caacatcctg tagcccggca acatacttat ctgcctgcga cacctgcccc
    10981 cgtgtggttg tgtcaatctg cccggtcacc tggtcgcttt gatttgcatc cattaccgca
    11041 ttaaaacgac cactggatgg gtcaacgccg gactgagcaa gattacccgc cagctccctt
    11101 cgcgcttcac caaactgttt ctgataacca agatttgttg tgccagcgat attttcgtac
    11161 tgctgctcac tgttaaggtc atcgaccttt tccatgaagt tatcttctgc cggacggagg
    11221 attttttgat aatcctgcca ccctttccag gccacttctt cctgcgctat ttgcgctgct
    11281 gttggtttta ctttggtatc accaccgcct ttacttccac ccataatggc cccctggata
    11341 acaaaaaacc ctgccggagc agggtcagaa tgtgaattac aatgttggtt taaacacaat
    11401 gatgaaaact atagcagtca gaccgctatc ctgaacacca tgaagccatc ctcatcatcc
    11461 ggcattcgct caaagcccag acgttttccc agccggataa atccccgcct tgccgtatga
    11521 aattcagccc agcgtccgcc agccagacgg gttaatgtct tcacctccgg cagataacgc
    11581 tcaacgctgt tactccccgt acacacgccc agcaacacca gaacataagg gataccatca
    11641 gcactgagca cagaacgcag caccagaaag ccatcaggtg cctcaaaaca aaacgcctgc
    11701 tttttaaggc aggcgtcttt aacttcattc ataaattcag ggttgcggga atttctcacc
    11761 acacgctgca tataccggag aattttatcg ttcattctct cacctgaaac gggtgccata
    11821 tcggctgaac ctcagcaacc agttgacggg gactttcgtc cccgccgcgg ttttcctact
    11881 gcttacactg taagaacgcc gcaaactccg ctccccacaa attcagccgg aactcacaca
    11941 acgagccgtg taacatccag atggtgaaga taaccgtcat acaaattgtg acggtgataa
    12001 gcgatttttg cgacatagcg cttgcctcct gagtggagag gcgctaacct tctacttgtc
    12061 aaggtacgaa gtgttagggc ctcgggttaa cgttaagttg actcggggcc tttccacatc
    12121 aggccttcag gttctccctc cagccatcag ccgaaaggca cccacgcata atttacggtt
    12181 tttgccctgt acgggcaata aaaaacccgc catcacagcg ggtagtaaac ggttaccgga
    12241 tacaggtcag aatttcaggc caatgccagc agtaacgcca ctcgtgcgcc agtcgccaga
    12301 gccggaacct tcgtaagcca catccagtgt aacactctca ttcaggttaa actgtgcacc
    12361 tgcagcccac gcaagggagg tttttttcgt gctgttgctt tcagaaaatc cgccactact
    12421 gttaatattg tccttaattt tcaggtcagc gctaacttta gcaacgccca tccctgccat
    12481 cgcatataaa ctcatgtact gattaacccg ccatgaaggc cccgctaaca gactccagta
    12541 attcgccctg atatccgttc tggcagaagc tgccggattc ttaatcttcc tgctatgatc
    12601 ggctgactgc acatcaataa atgtctgtga gttagtgaga gaacgcgtcc atgtaaaaga
    12661 ggtgataacg ccaaaatcat ccgttatctc gtagcgatac ctgatattaa tgccctgtgg
    12721 atttttatcc ttgccttcat atccactgac ccgaccatct gtatattcgc ccaatgaaga
    12781 aaagtagttt ctgttgacga aatgactgaa agtctccctg ttatgagcag tcgcatcctt
    12841 tacaaaatcc ttcagtcccg gaaactgaaa gtgcgcatac cccagagaaa ttgtatggtt
    12901 tccttctgct gcatgtgcag ataaatttac acaggcaatc ccggagattg cacacacaac
    12961 cagtgttgct atactcttca tttaagatat cctgtattca gataacacgc taaacttttt
    13021 aataatacat ctttttagac attttaaatg aatattaaag actcgcttca actaatcata
    13081 tactgatgta ttaataacca ggaccttatc tatatacggc tttcgaatat cccatgttgc
    13141 accaccggaa taatgctcac aggaatatat tttatttcct gtagcaccag tggatgtggt
    13201 ataaatcggt cgatcataag gagttctttt ccagttataa tacccgacca gtgcaggcat
    13261 gattgcgcat ggatatccaa ggtccttttc aaatttgata tcaacaggta ttaacttcgc
    13321 gtcaagcagc atcatttcgc catgataaat catctcaccg tccgggttat acatggcgat
    13381 accatactca gaaggtggag taaccatatt cgcgaaagca taaaccgtcg taacaccagg
    13441 gttcgttccc ctgacaatta catgaagcct tagtgcatga tatccatcaa tctgttcatg
    13501 cgtgtacatg acatcggcct tcttctctgt tctgataaag aaaaaacaac ttttgcctga
    13561 cgggattgat gttttaaaaa aagacttttc agtcgccggt atggtgcctt tgttgatcag
    13621 acactgcggc gtaaaacctg gacttatcca tacgctgcca tccggcttca taatgctcaa
    13681 accatacatg acacttatcc ccagaatgta taaatataag atcccatacc ctgctcaaga
    13741 ttcgaccacg tcaccgtatt gccattaatg gttatcttcg gtactttccg atccgcaaat
    13801 acattattcc agggaaataa acaacataca gcctgcaatg atttcccgtc gggtttattc
    13861 gtgtacgtct ttgagccaga ctccgctgta aatctgtcca ggaaaaatac cggagtcaac
    13921 acgcccgtaa cattaacgtt atttctgtta taaatggcaa aaccgtattc caatactcac
    13981 ctcctgatca gcgtaatctg cctatgcgaa cagccagtcg tccattctga tcataaacct
    14041 caattttatc attgcggatc accagtccta cattctgatt agagtaacga attgtcagtt
    14101 gcccttgtga cgtaacactg aaaaggcctc caatattcag gttaccctga gaatcaacct
    14161 gaaagtttcc gttctgaata acggcactcc ggataactgg cgaagtgata cttaccccgg
    14221 cttttacctc atccgccaca accttccgcg acaccagtgt ttcaatcacc gcgtcataaa
    14281 tcatcgcttt cgggatcaca accttgccac ctgataccgc aaacggatag gcggtgttat
    14341 ccgggttgtt cgggtcaaag acaaacaact gcgacgcaga aattgcaacc tgacttacag
    14401 gcctgccttc actgtctttt ccggcgacaa tcccgatccc cgcagtgata ccatcaactc
    14461 ccgctttttt tgaccacatt gcaagaaaag cttcaccgcc ttctttatcc aatttagtga
    14521 tgcgcttgtc gacctcatta agcgattcac tggttgacga atccagtgtg ctaatgcggg
    14581 tttcaatacc accaatcgtt cttgtcgttt cttccctgag agtccccaca acttcggttg
    14641 tcttaattgc agcatccttt acagcctgcc cctgcgcgtt ttttatttct ttacgcagct
    14701 cggacacaac cggcgacttt gcagcctcat cgcggatctg gtcaatgatg gccttcacgc
    14761 cgatctgtgt ttgtgcctga gtgccttttt cagcattcca tggacctttc actcctgccg
    14821 cgttaacaaa acgtatccag taaaatcccg accagccagg gtcaaccgga tcgccgtaaa
    14881 cctgccccgg cgtcgtggca accagcactg catcagcaag gtcatcctcc gtaccccgcc
    14941 agatttcagt cagtgaatgt ccgcgataat taggcatatc ccattcaaga agaaccgagc
    15001 caaatcctcc tgtcgcctta aaattcagcg gttttgtggg aaaatcaaca gtcattaaag
    15061 tactgtcaat ctcaataccc ggattcagtg catatgaggc accacccgat gttcgacgcc
    15121 gggcgagttt aagacctacc agttcttcac gggtcacaaa cgcgtgacgt ccgtcaccac
    15181 gctgcccggt gccaatttcc atattctcca caacagtgga taaatccttc cccgcccgcc
    15241 acggtttttt gctcatatcg gtaactccga catcgatgta ctcagggtta ttcgttccac
    15301 ctgcccgaat ccggatacca tcacctgcca gttttgcccg gttgctgccg gaagtctcac
    15361 cacacttccc ttaaacgtac ccggcgcaaa atgaatcaca ggaacatcat cagccataat
    15421 ggtgatcccc acccgctcag gcgccggaga tttcaccctg atacaggaaa aagaggttct
    15481 ttcaggtaat gaaaaaattt ttgaatgcca ccttatcgtg gagggcagag agcccccggc
    15541 aagcactgac attttgtctc ctgtcaccac acgcatcata tctttcgcga gatcaaccca
    15601 tgcgcagtca aacggtgtac tgagataacg gatatccatg ttcaccggac tgaatacaaa
    15661 cacatcctgc ttaccatccg gtttcgtgta acaggcaatg tactcaccac gccaggaata
    15721 agccacaatg gacgccgggt taaactgact ctgccactgt tcaggtgaaa taatcttttc
    15781 cgttgccagt gctgtattac cgtttacatc aacagatacc agaccgtttg tcccggcata
    15841 gagtacgaat ccctccatcg ccaccatact tcttcggctc aggcatgcct gcatggaagg
    15901 aattctggag ccagaaatgg tggaaggcga tacaccactg aacagataag gctccccctt
    15961 tgtcgccacc accagtgacg ttcccagcgg acagatagcc acaatatctt ctgccgtcgt
    16021 gtgacgattc acttccggcc atgcatacgg cagatacgct tccgaaaaca tcacttcatt
    16081 accggcaaac ccggcggcaa taccattagc catcaggcaa aggcctgtca tattctctgg
    16141 cggcggcagg taatcccatg tagccagaga aggtccaagg tttttccccg gtattttgtc
    16201 cgtgtaactg agcacggatg catccagttc agccacaagt aaaaaatccg cttctcctcc
    16261 acctgatgca gagcgataaa tccggcggcg tttaatactg gcattctgca atggcaccgg
    16321 agacagcgtc agctgtaccg cagtccccgg agtacggagt gttacctcca gagacgccgg
    16381 acctggcgga ccttcttcac cataatctga gacaaaggtt tccgtataaa accgggtttc
    16441 gtcatcattc gggttatcgt cagaaacatc accgccctgc tgaacagtac agacaggtgc
    16501 tgtcgtcggc gcggggatcc ccagacgata cgatgatgtc gggtgattcc cgtccccttt
    16561 tgtggcaatg gtcgcatccg tcactttagg aaaacgcccg tcagtgtagt aaatacgccc
    16621 gtgggagtcc tgagcgaccg gactgcggat cacatccacc acatccgtcc atgcaaacca
    16681 gaaatcgtca cggtaatgaa aaatggtttt cggcttaatc gcgaatgttt tctcagcctc
    16741 tgacatctgg tgttcaggcg tgatcactcc atagcgaaaa tgacagtttt ctgccagtac
    16801 agcagaatga tctggcagca tagatgcaat aacgcctggc atcatcccgc gcatagttgt
    16861 tatatcgata taaggcatgg ttgtctggta tccgaaaatt tgaatggcag gtgagtcact
    16921 gaattgaagt tttaaaggcg gcatacaatt gcgcctgtgc agaatacaga tttccagaaa
    16981 tctgaaaaat tactcttatc agagaagagc gtcagaaaca cgatcggcat cactgtttct
    17041 gcaaacttcc ctgtggcagt ctttattcgc catataacgt agcactaaag gtcttcatta
    17101 atttttccat tgtagttact ccttccttgt accgggggtt ggtcatgccc ccgtttttta
    17161 ttccctctgc tcctttaatc gcactgccag gcgccacaac acacatcaac tgtggctggt
    17221 atggaaacac caaaaactct cacttccgga aatctgaaaa attatccata tcaacaggat
    17281 gcgccggaaa catgatttcc atcacatcca ggcctgcact ttccgtgaca gtatttattc
    17341 gcatagtaaa tacttcacaa gtgtataatt gactcagttc atgggggagg tgatggtccc
    17401 ccatcttttt gtctgttatt cctgtgccca accaaccctg tatgccagta tctcatccgc
    17461 actggtcagc ttttccagct ctttcttcat ggtgcgctgg cgaacgtgga tttccatccc
    17521 tttggtgaac atcgcctgct ctgccgcttc actcagcgca ataatctctt ctgctgtcac
    17581 cggcacatca ttgttttccg catccgtcca gaaaaacgcc tccggcagtt tccccgcttt
    17641 cgctgccgcc accgacggct caagacgcgt ctgcgttgac ttcccgtagt cccatttacg
    17701 cccattgtgc tcaaacgtgt agttcgccgc ttccatctca ttacgccagg cgttaatttc
    17761 atcgcccttc atcccgcgcg ctttctccgc
//
LOCUS       DAZGCQ010000098        17447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000098 DAZGCQ010000000
VERSION     DAZGCQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.098"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1330
                     /gene="xanP"
                     /locus_tag="ACHK07_003793"
     CDS             <1..1330
                     /gene="xanP"
                     /locus_tag="ACHK07_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO8104075.1"
                     /translation="LIYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPA
                     QDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGA
                     DVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSI
                     GGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFM
                     GMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSE
                     QPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVV
                     ALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIA
                     LSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1451..3160
                     /gene="yicH"
                     /locus_tag="ACHK07_003794"
     CDS             1451..3160
                     /gene="yicH"
                     /locus_tag="ACHK07_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8104076.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(3204..3884)
                     /locus_tag="ACHK07_003795"
     CDS             complement(3204..3884)
                     /locus_tag="ACHK07_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO8104077.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEASLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(3865..4797)
                     /locus_tag="ACHK07_003796"
     CDS             complement(3865..4797)
                     /locus_tag="ACHK07_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO8104078.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(4845..5705)
                     /locus_tag="ACHK07_003797"
     CDS             complement(4845..5705)
                     /locus_tag="ACHK07_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO8104079.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANLDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(5786..6637)
                     /locus_tag="ACHK07_003798"
     CDS             complement(5786..6637)
                     /locus_tag="ACHK07_003798"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8104080.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAYAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADMMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            complement(6649..7740)
                     /locus_tag="ACHK07_003799"
     CDS             complement(6649..7740)
                     /locus_tag="ACHK07_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8104081.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(7765..8079)
                     /locus_tag="ACHK07_003800"
     CDS             complement(7765..8079)
                     /locus_tag="ACHK07_003800"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8104082.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(8097..8567)
                     /locus_tag="ACHK07_003801"
     CDS             complement(8097..8567)
                     /locus_tag="ACHK07_003801"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO8104083.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(8594..10147)
                     /locus_tag="ACHK07_003802"
     CDS             complement(8594..10147)
                     /locus_tag="ACHK07_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO8104084.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVKIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(10442..12760)
                     /gene="yicI"
                     /locus_tag="ACHK07_003803"
     CDS             complement(10442..12760)
                     /gene="yicI"
                     /locus_tag="ACHK07_003803"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO8104085.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANAQGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEVPAADGSVIFTLKATRTGNTITVTGAGEVRNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(12770..14152)
                     /gene="yicJ"
                     /locus_tag="ACHK07_003804"
     CDS             complement(12770..14152)
                     /gene="yicJ"
                     /locus_tag="ACHK07_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO8104086.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISIAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIVAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            14445..14539
                     /locus_tag="ACHK07_003805"
     tRNA            14445..14539
                     /locus_tag="ACHK07_003805"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14479..14481,aa:Sec,seq:tca)
     gene            14618..14779
                     /gene="rhuM"
                     /locus_tag="ACHK07_003806"
                     /pseudo
     CDS             14618..14779
                     /gene="rhuM"
                     /locus_tag="ACHK07_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            15177..16361
                     /gene="setC"
                     /locus_tag="ACHK07_003807"
     CDS             15177..16361
                     /gene="setC"
                     /locus_tag="ACHK07_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO8104087.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARNSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNVPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            16472..17395
                     /gene="yicL"
                     /locus_tag="ACHK07_003808"
     CDS             16472..17395
                     /gene="yicL"
                     /locus_tag="ACHK07_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO8104088.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPSALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
BASE COUNT         4359 a         4544 c         4080 g         4464 t
ORIGIN      
        1 actgatttac cgtcttgaag atcgtccgcc gcttcctcaa accctgtttg ccgcctgtca
       61 gcatttgctg gcgatgttcg ttgcggtgat cacgccagcg ctattaatct gccaggcgct
      121 gggtttaccg gcacaagaca cgcaacacat tattagtatg tcgctgtttg cctccggtgt
      181 ggcatcgatt attcaaatta aggcctgggg tccggttggc tccgggctgt tgtctattca
      241 gggcaccagc ttcaactttg ttgccccgct gattatgggc ggtaccgcgc tgaaaaccgg
      301 tggtgctgat gttcctacca tgatggcggc tttgttcggc acgttgatgc tggcaagttg
      361 caccgagatg gtgatctccc gcgttctgca tctggcgcgc cgcattatta cgccgctggt
      421 ttctggcgtt gtggtgatga ttatcggcct gtcgctaatt caggtggggc tgacctcaat
      481 tggcggcggt tacgcagcca tgagcgataa caccttcggc gcaccgaaaa atctgctgct
      541 ggcaggcgtg gtcttagcct taattatcct gcttaaccgt caacgtaacc cttacttacg
      601 cgtggcctca ctggtgattg cgatggcggc cggatatgcg ctggcgtggt ttatgggcat
      661 gttgccagaa agcaacgaac cgatgacgca agaactgatt atggtgccaa cgccgctcta
      721 ttacggtctt ggcattgaat ggagtctgct gctgccgctg atgctggtct ttatgatcac
      781 ttcgctggaa accattggcg atatcacggc gacctctgac gtttccgaac agccagtgtc
      841 cggtccgctg tacatgaaac gcctgaaagg cggcgtgctg gcaaacggcc tgaactcgtt
      901 tgtttcggcg gtgtttaaca ccttcccgaa ctcctgcttc gggcagaaca acggagtgat
      961 ccagttgacc ggtgttgcca gccgctatgt cggttttgtc gtcgcgctga tgttgatcgt
     1021 gctgggtctg ttcccggcag tgagcggttt tgtacaacac attccagaac cggttctggg
     1081 cggcgcaacg cttgtaatgt ttggcaccat cgccgcctcc ggtgtgcgta tcgtttctcg
     1141 tgagccgctg aaccgtcggg cgattctgat tatcgccctg tcgctggcgg ttggtctggg
     1201 cgtgtctcag cagccgctga ttttgcagtt tgcccctgaa tggctgaaaa acctgctctc
     1261 ctccgggatc gccgcgggcg gtattactgc catcgtgctg aatctgattt tcccaccaga
     1321 aaaacaataa ttcactggcg gcggtaagat aattaccgcc gcagtctcac ctctttcacc
     1381 attcccgcct tgaggattgc gcgtaaatcg tgcataactc ccttatgtgc atttcacggg
     1441 atggaagacc atgaaattta ttgggaagct gcttctctac attctcatcg ctctgttagt
     1501 ggtgatcgct ggcctctatt ttcttctgca aacccgctgg ggagcagaac atatcagcgc
     1561 atgggtttcc gagaatagcg actatcatct ggccttcggg gcgatggatc accgtttttc
     1621 cgcgccatct catattgtgc tggagaacgt cacgtttggt cgtgatggcc agcccgcgac
     1681 cctggtggcc aaaagtgtcg acattgcgct aagtagtcgg caactgaccg aaccacgcca
     1741 tgtcgatacc atcctgctgg aaaacggaac gctgaatctc accgaccaga ccgcgccgct
     1801 accgttcaaa gccgatcgtc tgcaactgcg tgatatggcg tttaatagcc cgaatagcga
     1861 atggaaactg agcgcgcagc gggtaaatgg cggcgtggtt ccgtggtcac cagaagccgg
     1921 taaagtgctg ggtacgaagg cgcagattca gtttagtgcc ggatcgcttt cgctcaatga
     1981 tgttcctgcc accaatgtac tgattgaagg cagtattgat aacgatcgcg ttacgctgac
     2041 taacctgggt gccgacatcg cgcgcgggac attaaccgga aacgcgcagc gtaacgccga
     2101 cggcagctgg caagtggaaa atctgcgcat ggcggatatc cgtctacaaa gcgaaaaatc
     2161 gctaaccgac ttctttgcac cattacgctc tgtcccgtcg ttgcagattg gtcgcctgga
     2221 agtgatcgat gctcgtctgc aaggtccgga ctgggcggtg accgacctcg atctcagctt
     2281 gcgcaacatg accttcagta aagatgactg gcagacacaa gaaggcaaac tgtcgatgaa
     2341 cgctagcgag ttcatttatg gttcgctgca tttatttgac ccgattataa acgcggaatt
     2401 ttccccgcag ggcgtagcgc tgcgccagtt caccagccgc tgggaagggg gtatggtcag
     2461 aacgtcaggg aactggctgc gtgacgggaa aacgttactc cttgatgatg cggcaattgc
     2521 cgggctggaa tataccttgc cgaaaaactg gcaacagttg tggatggaaa cgacaccggg
     2581 ttggttaaac agcctgcaac tgaagagatt tagcgccagc cgcaatctga tcattgatat
     2641 cgaccctgac ttcccgtggc agctcaccgc gctcgatggt tacggtgcca acctgacgct
     2701 ggttaccgat cataaatggg gcgtctggag tggttcggcg aatctgaatg ccgccgccgc
     2761 gacgtttaat cgtgttgatg tccgtcgtcc atcgctggcg ctaaccgcca acagcagcac
     2821 ggtgaatatc agcgaactga gtgcatttac tgaaaaaggc attctggaag ccaccgccag
     2881 tgtttcacaa acgccacaac gtcagaccca tatcagcctg aatggacgcg gtgtgccggt
     2941 gaatattttg caacattggg gatggcctga attaccgttg actggcgacg gcaatattca
     3001 gcttaccgcc agtggcgata ttcaggccaa tgtcccgctg aaacctacgg ttagcgggca
     3061 attgcatgcc gtgaacgccg caaagcagca agtgactcaa accatgaatg cgggcgtagt
     3121 ttccagtggt gaagttacgt cgacggagcc ggcgcagtaa aaataaggac gctagcggat
     3181 atcagcgcta gcgttccccg ttattagcgc aaaattccat actcactgca caataaccat
     3241 ttcaccggtt catcttcttc tatcgcagga aaacgtcctc ccggctcaag aaaatagtta
     3301 tcgtgttggt catcatttac catcgaaatt tcccagccga gaacaaacgc tttttccgcc
     3361 cagaagcgat gatacagacg cggcggcagg caaatgccct gccccggctt taacaccagc
     3421 gtttcaccag gtttcatggt acgccgctga ccatcaacac tcacggtaat gactcgttga
     3481 tcatcacaga gactggcttc gttggcccag gccaactgca tgcataagtc tccaccgccc
     3541 cgattgagga tatcttccat tttgtgggtg tgaaaatgcc agggagtctg ctgatcttgt
     3601 tggatctgca acattttctc agcatacggt tttggatatg ccgccgaccc cagcgcgccg
     3661 ttgcgcatcg ttagcagtgt tagtcccgtt tggttgaaat caccacgacc aaaatcggtg
     3721 atatcccaac cgagctgtaa gtcgaccact tcttgccagt tatcccgttc ccgctgcagc
     3781 cagtcatcca ccgtaaaata ggcgaactct ggcaaacaaa cgcggaaggt ctgcgcaatg
     3841 gcatgtgctt tatcaataac ataattaatt tgtgaacgtt tcattggctt ctccgttgcg
     3901 catagcaagc caggcagcgc cacgcattcc gctactgtcg ccgtgctgtg cttttacaat
     3961 gggagtggtg aattgatcgg tgaaaaccag tggagcgact tctgcgttca gatcggtgat
     4021 aagcagttcc acattcgaaa gcccgccgcc gatcacaatc acgccagggt caacaacatt
     4081 aacgatggtt gcgagcgtgc gggcaagctg ttggcgaaaa cgtgccacct gctgcatggc
     4141 acaagcatca ccacgttgtg ccagggtgac aatcgccgca ggagtgagca agttaccagt
     4201 catctgttga tagcgttcac taaaaccgct gccggaaaca aacgactcca cgcagttgtg
     4261 tttgccgcaa tagcaactga ctgatggacc atcttcctgc tccgtatagc ctggcaacgt
     4321 gatatgaccg cattcggcag catttcccca ggcccctata aacggtcgtt gcttgatggc
     4381 aatgccaccg ccgcagcccg taccaagcgt aataccaaat accacgtcat aatcttgccc
     4441 ggcaccgtcg caagcttctg atagcgcgaa acagttacca tcattggcta tcaccaccgg
     4501 ttgcccaagc aattgctgca aatcagcctg taacgcatga ccgttaatca cctgaatatt
     4561 cgcgttttta atcaggccag tgagtggcga gatactgccg ggtaaggcga tccctgtcag
     4621 catcggtcgt tgaacgtccc gcctaatctg ctcgataagt gccacaacgc atgagacaaa
     4681 ttgttgatat gtcgactttt gcgtcgggca gcggtaacgg cgaatctccc agccatgcgc
     4741 atccatgacg acggcggcga ttttggtccc accaatatcc aaccccagat agtgcatatc
     4801 tcgttccttg ttgcgtaaaa ggcgggtcac cccgcccttc tggcttagta cagtgatgct
     4861 ttaccgagcg aaccgaacag tttcattttg tggcgaatga cttcccgcgc cgcgttaatc
     4921 gcatccggat agatgacgtt cgggtcccac cagctctctt tagcgagaat ttcacgcact
     4981 ttctggaaat aggcgtattt catatcgctg gagatattga ttttgccgac gccgagcgta
     5041 actgattcag caatctccgc gtccaggttt gccgaaccac cgtgtagtac cagcggaata
     5101 tcaatacgtc cggcaatgtc gcgcaaaatg ttcatttgca gcttcggttg catccctttt
     5161 ggatagatgc catgcgctgt accaatcgcc acagccagtg tgtcggcacc tgtacgagca
     5221 ataaaatcgg cggcctgcgc cggatctgtg taggtcactt gtgaaacacc accttctacc
     5281 gacgttcctg tttgtccgat ggttcccagt tcaccctcaa ccgacacgcc aaccgcgtga
     5341 gcgaggcgaa ccacttctgc ggtcagcgcc acgttttctt catacggcag cagcgaaccg
     5401 tcgatcatta ctgaggtaaa tccgcagcga atcgcacgca aaacatgctc tacagaggca
     5461 ccatgatcga ggtgcagtac aaacgggact ggactacgct gggtaatttc gcgaacgtga
     5521 gcgaaaaagg catcgcccac aaaatcatgt tcgctgggat ggatggcaat aatcgccgga
     5581 gtatttgtcg cttctgcttc atccacgact gcgcgaagga aacagttatc cgccacatta
     5641 aatgcgccaa tggcaaagcc gtgctggcgg gtaggttgca gtaattcttt catcgaaact
     5701 aacatgttaa atctccagtt tcagcgtgta gtccgccctg ccggtgcgtt attgcactgg
     5761 cataaaggcc atgacagaaa atgaatcagg ctttgccgat agagccgctc gcctgtaaat
     5821 acggtgtaat cgcttcttcg aaggccagtt ccatagcttt cagcgcgtcc accagttcaa
     5881 tggatatatc gtgacagagg gtatgcagga gcgcggattt cccccgttca aaaatggccg
     5941 cccccacgtt gatttttgct acccccagcg tcgtacagag ctggagctga tcgaacggcg
     6001 taccgctacc gccatgtaat gccagaggca cgctcgagca gtggaaaatc tccgccagac
     6061 gctgaaaatc aatagcaggt acaacaccag gcgcgtacat gccgtgcgcc gtacccacag
     6121 agacagccag tagatcaatg cctgtctgcg cgataaacgg ttccacatca gtgggttgcg
     6181 tcatcatgtc tgcattggcg gcctggtcgt aacgcggcgc atctgccaga tggcccagtt
     6241 cgccttccac gcagagctgc gcgctggcgg cgagttccac caccgccctg gtctggcgca
     6301 cgttttcctc caggggaaaa gcagaagcat caatcattaa tccgctgaaa ccagcacgaa
     6361 acgcctgacg aatacagtcc ggatttttgc catgatcaag cgccagagtg accggtactg
     6421 aggcagattc cgcgagcgtt ttaaccactg ccgccgcgac gggtgcagga aaatgtgcca
     6481 gatgcccctg atagagattg agaatgatcg gagcgcgatt cttctccgcc acgcggatag
     6541 ctgcgtaagc actttccaga ttcatacagt tgatcgccag caaggcgtaa tgttcatgcc
     6601 aggctttcat caccatactt ttcatatcgg caaacatagg gcctccggtt attcgagggt
     6661 gaaagtgatc tcttcatctt tcagcttttg atcgctcagt tcgtcaaact cttcgtcgcg
     6721 ttccgttacc ggtttcttcc agagtgaaag catcacgcca caaatcaccg tgcctatacc
     6781 cagcgccagg caaaacagca gaggatgggt gaacagaggc acaacaaaca tgccgccatg
     6841 cggaaccgga ctctccacgc cccacaccat gattaagccg cccgcgaccg ccgatgccac
     6901 cacacaagat cccactactc gcagcaggtc acgcgcggcg atcggaatga ctccttcagt
     6961 gatcatgcag atccccatcg gaaacgcggc tttcagcgcc tcgcgctccg cacgggtgta
     7021 tttatggcgt gtcagtaaga acgaaagtgt gatgccaaac ggagggatga ttgagccgac
     7081 aaatttcacg gcttctggtc cgtagacgcc actgaccagc aagccatcgg aaaacagcga
     7141 catggttttg ttgaccggac caccgaaatc gaaggttgcc atcgcgccaa gtatggcccc
     7201 catcaggaag cgcgatccgc cctgcatcga ctcgagcaaa tgaatcagtg cttcttgtaa
     7261 ccaggcgatg ggtttaccaa tcagcgtcat cattaacagc ccaccgataa tggtgcttaa
     7321 taccggcaac accatgattg gcatgagtcc ttgcatcgac tgcggcaggc ggatatagcg
     7381 tttaagtagc aaaatggtgt agcccaccag gaaaccaccc aacataccgc caataaatcc
     7441 ggtatggatt tgcccacaga gaaagccgac aatcagaccc ggggcaaatc cgggacgatc
     7501 ggcaatagaa taggcaattg ccgcgctaat cagcggcaca atcagcccca ttccccagcc
     7561 gccaatctga ttaagcatcc acgggattgt tcctgttttt tcaccaacat ctgtaccgcc
     7621 aagaacttgc cccaacgcaa tacatatccc ggcggccaca ataaggggta tcatccacga
     7681 gatgccggtt aataaatgtt ttttaatttc ctgtgaaacc ggaattttat tgtctttcat
     7741 attaagatcc ctccagtata aggatcattt gtttcccagt gatttttcga ccttttcaag
     7801 aaattgaacg ggcgatttaa tgacgatctc tgtctttact cgcacaatac gtttattggt
     7861 gaagcgttct tctcctttta ttttcacatc tacagcaaga atcacgacgt cagcagcaga
     7921 gatatctgcc gcctgtaatt cattttctgt accaatggtt ccctgggttt caactttaat
     7981 ggtatggccc agagcttttg ctcctttgat taacttttca cgagcaatat aggtatgagc
     8041 aatacccgcc gtacaggcgg caacacagac aatattcatg acatactccc tgttatttag
     8101 cagacatcgg attgtgcata ctgactaaag agatcaatga ttttttgcgg actcgtctca
     8161 actaacaatt gttctatgat gtcatcatcc gccagcgcgc ccgcgacctg ggaaagtaaa
     8221 cggatatggg tggtattttg atcttcaaga cgtaccgcaa ataaaataat acagcgcacg
     8281 ctactaccat ccagcgtttc ccagggaata tcctggcggg tacggccaat tgccagcgta
     8341 gtattaatta ccgcagaaga tttaccatgc gggatcgcaa tatgattttc aaaaccagtc
     8401 gagccttccg cttcgcgctg ccagacatct ttaataaagg tttcccgaca atcgatcgca
     8461 ccatcttttt tgagaagatt agttaattct tcaatcgcct ccgctttatt cgtcgcttgc
     8521 atatctaaaa taacgcgatt cgtattcagg atttttgtta tgtccattcg tcatacctca
     8581 taaaattctg acattaaatt tcaacgaccc gagacgtcgc atgatgtaat ttttccgtaa
     8641 tcaccatttc agtgacccgc ttaatatcgg catcattaaa aaaagcggta acataagcga
     8701 ccggcatagt gactgtaggc aaattaatgg tcgaaataat cagttcgata ttgtcaggca
     8761 aaacctgtga taaattcgct gctgaaataa catcaacaat cgtccattca ggaaatgctc
     8821 gcagaatacg gcttttcagt agatgcgaag ttccgattcc ggttgaacag accagtaata
     8881 cacgtttacg ggcaatttgc cgctccatcg cagcctgaaa atgaacggtc aggtaaccca
     8941 cttcatcctc gctcactgca ttctcacccc aggttttaaa tacctgattc accacatatt
     9001 gcgtcaaccg ccagacatcc gctaattctg ctttaatatc ttccagcaat ggattacgga
     9061 tatgaatacg ataatttagt cggttaatca gcggtttaat atgcaccaat aagccatcat
     9121 ataatgcgct gtcttcacta aaatcgcagt ccacgatgtc actgaatacc gtaattaaac
     9181 gccaggtaat aagacgcgct tcattgctgg cctgcatatg tgaaataatg ctaacatctt
     9241 tttgtccatc aatcgccaca cctgatgaaa tgatatattg ataaataaac cagacttcat
     9301 catcgggcaa tgtatgtgca attcgttgtt ctatcttatg aatcatctga ctggcaacat
     9361 taaagatatt ttcatcatat tgcctggttt gatcttcttc tcttgataac gcattcccgt
     9421 gcgtgttgcg atacatcata ataaggatgt gagtaaaaat gttaacgtaa taaggttctc
     9481 ccaaagacca acttaattca ttttccatat ccagtaacaa tgactggaca aataacacct
     9541 cttcttctcc aaaataatgg actaatgctt tataacttcc gggatccaga cgtgaataca
     9601 cgacaccttg cggctcatta tggttaataa tgccgttaat cagtaatgcc attgcctgtc
     9661 ggacttgccc ttcactacct tcaatatgcg taccacttgg gctgcggatc aacgataacc
     9721 ccaacggcgc aagccaggac tcaattactc tcaggtcatt aacaatagag gctccgctga
     9781 taaagtagcg ttctgacaat ttactgatcg acgtttcatt cggtgtttca cttaataact
     9841 gcgacgctat tttaacgcgc ctggcattat tcattacacc atcaatctct tcacattctg
     9901 ctcccaacaa atgatcaaga tgcagcaaat cagcaatatt ctccgcgctt aacatcacac
     9961 ccgcgccagc acgcttctca acatttattc tccattgccc cagccattgc tcaagtaaac
    10021 gtaaatcacg ttgaatggtt ttttccgaaa catttaaata gccggcaatc ttaacaatag
    10081 tcgtatattc ccttcgaggt aaaaggaatc gtaataatct gttttgtcgc gaggtaatca
    10141 tttgcatttt ttacccttat cacagattaa gaagccccat gaggaagagc ttcagttgcc
    10201 atctctgagg gtaaatataa agcagaataa tttcacaaca atactaaaat ctgtccgtaa
    10261 tccggacagt gataaaacaa agcaaggcgg ttaagggata tttttcagca cagatcacat
    10321 tttcgcgatg aataatcgtg atcatgctcg caaatacgat ggaagaaata tttattacca
    10381 gatgtgagat tatttaccgg atacgacacg aaaccattcg tatccggcat tcttcaatag
    10441 attacaacgt aattgtcagc gcattccctt gaggcgtcac caccagcccc tgctcacttt
    10501 cagcctgcga accgccttgc agaccattta ctttcacaat attgcgcaag cacagtgtcc
    10561 agttcctcac ctcgcccgca ccagtcacag taatcgtgtt accagtacgt gttgctttta
    10621 gagtaaagat caccgaaccg tcagcagcgg gcacttcaca gacggcttca tgcccgtctt
    10681 gcagattaaa gagctggaat gccgtgcctt cgtgccacgc gtaatcggga cgttgatcgt
    10741 tgttgcccag cgccagtagg gtgttatcac gcacataaat gggcagactc aggaagctgt
    10801 gctgctgttt atgccagcga ctaccgtcga gctcatcgtt gtgccacagg tgtgtccagc
    10861 gaccttctgg caggtagaac tgcacatcgc ccgcttcagt gaacaccggc gcaaccatca
    10921 cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca agccggatcg tccgggaact
    10981 ccatcatcat ggcccgcatc atcggcgtac cctgcgcgtt cgcacgagca gcttcacgat
    11041 acagatacgg catcatgcgg catttcagtt gcgtgaagaa gcgcaccaca tcacaggact
    11101 catcatcgta ggcccacggc acacgataag atttgctgcc atgtaaacga ctatggctgg
    11161 agagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc cggagcggta ttctcaaagc
    11221 cgccgatatc gtggctccag aagccaaaac ctgaaaggcc aatagacaaa ccaccgcgca
    11281 gactttccgc cattgattcg tagttagcgt aacagtcacc cccccaatgt accgggaatt
    11341 tctgcgcacc gacggaggcc gagcgggcaa acaagacggc ttcttcctct ccgacggttt
    11401 ccttgagcac gttccacacc agttcgttgt agatgtacgc ataatggtta tgcattttct
    11461 gcgaatcgga accgtcaaac cattgaacat cggtaggaat gcgttcgcca aagtcggtct
    11521 taaagcaatc aacgcccatc gcgaccagac ctttcagttt gtcggcgtac catttgcagg
    11581 catccggatt ggtaaagtca taaatcgcca gacctggctg ccatttatcc cactgccaca
    11641 gcgaaccgtc cgggcgtttg agtaaataac ctttctcttg taactcttta aagacggggg
    11701 atttttgccc aatatagggg ttaatccaga cgcagatttt cagccctttc gctttcagac
    11761 ggcggatcat cccttccggg tccggaaaag tcagcgggtc ccactcaaaa tcgcaccact
    11821 ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg
    11881 ccataccatc aataaagcta tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg
    11941 ttagccacag accgaaggac cacgcgggcg gcagcgccgg acgaccagta aagcgggtat
    12001 aacgatcaag taccgctttc ggcgtcgggc cgtcgataac aaagtattcg agatattcac
    12061 tctcaacgct gaactgtact ttggagactt tctccgatcc cacttcaaaa gagacgcatt
    12121 ggggatgatt gaccagtacc ccataaccac ggttagtcat ataaaacggg atatttttat
    12181 acgcctgttc agtacttgtg ccgccgtccc ggttccaggt ctctaccgtc tgaccattgc
    12241 gcaccagggc agtaaagcgc tctcccaggc cgtaaactgt ttcgccaacg ccaagatcaa
    12301 gccgttcaaa catataattt cgttgattat tcgtgtcctg cacgtagcca ttatttttca
    12361 cctgactacc ggtaatacgt tcgccgttgc gcagaaaatc cagtgaccag aactcacctt
    12421 tgctgacacg cgcgcttaag ttgccacttt taaactcagc ataacgttct gtgttttcga
    12481 ttgtgacctt cacgtcctgc aaaatattga gcggataatg agggccgtta ttcagcgccc
    12541 cctgaaaatg ctcaatccgc accccgacaa taccttcctg tggggagaaa aagcgcagcg
    12601 taaataaagg cgtgtcaagc tgccaggtac gttcacgtac atcacggggg gcagcataga
    12661 ccaccatttc attaccctgc tgttcaacct cgaatacctg aagcgggtga atcaaattga
    12721 ggccaggttg aatcaaccag tttccatcgc taattttcat tgccgttcct tagttctgca
    12781 cttcttgaga ggtgaattgt tgctggcaac ggcgtttacc ctgagccagc tgttccataa
    12841 cggttttcag attgtgcgtc gtgagtgagt agtagcgttt agcgacaatc gcgctcagca
    12901 aataacagat cgccggaaca atcgtgaata gcgcaataat gatgctaatc gtggcgctgt
    12961 tctgcgcttt ttctgccgca tcatatccgc cataagccag catccagccg ataagagcgc
    13021 cgccgaaggc caaccccagt ttgagcacaa acagcgtgcc agcaaaactg atcccggtca
    13081 ggcgtttacc attgcaccac tcgccgtagt cgacggtatc ggacatcatt acccactgga
    13141 taggtgtcac cagttgatgc aacacaccaa tcacgaagat gaagacaaac atagtgatgc
    13201 tggcctgcat gggaacaaag aacatcgcga tgctaatcac tgccagcagg gcgttcgtcc
    13261 accagaagat agtgacttta catttccagt cggtcagcgg ttttgccagt gcggaaccaa
    13321 tcaagttacc cacgcaataa gtggtgagaa aagcgacaaa cacttccggc gtacccaaaa
    13381 tccatgtgac gtaatacatc atcgctccac cgcgtacgca cacagccagg atattgaaaa
    13441 tggttagtaa accgacaatc cgccactggt cgttttgcca gatatcacgt aaatcttccc
    13501 gcatagacgt cgttgtaggt ggtgcttcaa cgcgttcttt agtggtgaag aaacagaatg
    13561 ccagcatcat gaatgccacc acggaaagga ccgcgatacc gccctggaaa ccgagtggtt
    13621 tattatcacc accaattaaa ttaaccaatg gcatcatcag aaccgtagaa agcatgcctc
    13681 ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat acgctgcgtc gggtcattgg
    13741 tgattacacc acccaatgcg caataaggga tattaacgac ggtataaagt aaggtaagta
    13801 gggtgtaagt aattgctgca taaatcattt ttccgttcat actgagatct ggcgtgctat
    13861 aggccagtac acagacgatc ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt
    13921 taccccagcg agagcgcgtt cgatcggcca acaaccccat gcaaggatcg gaaatcgcat
    13981 ccagtgcacg agcgaccaaa aacatagttc cgacaaatcc ggcaggaatg ccaaaaatat
    14041 cggtataaaa gaacatcata tataacatta cgttatcgaa aataatgtgg ctggcggcgt
    14101 ctcccatgcc ataaccaatt ttctctttaa cggacaacac ttcactcttc atctgctttt
    14161 ccttgatgct ataggggtgc tgagaccgat aacgcatttt gtaaaccatc cctgtgagag
    14221 cgaatattcc gatatctggt tattaattta tgattcttgt tttatgtgat cgtggtagcg
    14281 ttaattccgc tcatatatca ttgtaaaata tgggttttat atgaactata atgctttcgt
    14341 gataatactc tgcgtgtatt aggcggaaaa aactgatctg ggggatgtag aaactcaagg
    14401 aagtagctat aatgcgcccc gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc
    14461 cggtgaggcg gctggacttc aaatccagtt ggggccgcca gcggtcccgg gcaggttcga
    14521 ctcctgtgat cttccgccaa aatgcctctt catacgttct ccaacccctc gtaatactgt
    14581 caatccagat attttatact gctcaatatt caatcggctt caggaatacc tggtcaaagg
    14641 ttttgtgatg gacgatgagc agctgaagaa tccgcctgtc ggttcttctg tcctcgaacg
    14701 catccgcgat attcgcgcca gcatgcgccg tgtttattta caggtgattt tcccccctgc
    14761 tgactctcag ccaactgtat cgccattaag gtgaagatgt tgttttacac gctccagaac
    14821 accctttttt tatacgactg ttctattcct ttaacagctg gcaaaaaaac tgacctataa
    14881 acttcaccaa taatcagact gccatcaaga ttaatttgtt catttaatat atcaaatcaa
    14941 cttgttttat ttttactatc aaaaatagct cattgcctta ctataattaa tatataaatt
    15001 cggtgattaa ttcttaacat gcttttgcta ataatctcaa ttgctggccc tataatattg
    15061 cgctagcatt gttttctggt tgtatcagcg atactcaaaa attcttaata caatactcat
    15121 tcgactggta cttatttgta actcagttat attttgtcgc ccggtgattc agaagaatgc
    15181 aaaaaacggc taccactcca tcaaaaatac ttgatctcac tgccgcggca tttttacttg
    15241 tcgcctttct gacgggtatt gcgggcgctc ttcagactcc taccctaagt atattcctcg
    15301 cagatgaact gaaagcccgt cctataatgg taggtttttt cttcaccggt agcgctatta
    15361 tgggaattct ggtcagtcaa tttctggcaa ggaactccga taaacaaggc gaccgtaaat
    15421 tactgatcct gctatgttgc ttatttggag tgctggcctg cacgcttttt gcgtggaatc
    15481 gcaactactt cattctgctc tcaacgggcg tacttctgag tagttttgct tccaccgcaa
    15541 acccgcaaat gttcgccctc gcccgtgaac acgccgatag aacaggccgt gagacggtca
    15601 tgttcagtac atttttacgt gctcagatct cgcttgcctg ggttatcggg ccaccgctcg
    15661 cttatgaact ggcaatggga tttagtttta aagtgatgta tctcaccgct gccatcgcat
    15721 ttgttgtttg cgggctgata gtctggttgt ttttgccatc aatacaaaga aatgttcctg
    15781 tcgttaccca acccgtagaa attttaccct ccatccatag aaagcgagat acgcggctac
    15841 tttttgtggt ctgttcaatg atgtgggcgg cgaataatct ctacatgata aatatgccgc
    15901 tatttattat tgatgaactg catctaaccg ataaactggc tggagaaatg attggtatcg
    15961 ctgccggtct ggaaattccg atgatgttaa tcgcaggcta ttacatgaaa cgtattggca
    16021 agcgactatt aatgctcatt gctatcgtga gtggaatgtg tttttacgcc agcgtactca
    16081 tggcgacgac tccggcagtt gagctggaat tgcaaattct aaatgccatc ttccttggta
    16141 ttctctgtgg tatcggcatg ctttattttc aggacctgat gcctgaaaaa ataggctctg
    16201 cgacaacgtt atatgcaaat acttcacgcg tcggctggat tatcgccggc tctgttgacg
    16261 gaattatggt tgaaatctgg agctaccatg cgttgttctg gctggcgata gggatgttgg
    16321 gtattgcgat gatttgcttg ctgtttatta aagatattta gctcaccagt ctgggctaag
    16381 aaaaatcggc gtggcgcaaa cagagccatc ctcgccattt ttgctatcat gcctgcatac
    16441 ataaacgaca aaacagtatg cagagggaaa aatgggttcc accagaaagg ggatgctgaa
    16501 cgttctgatt gccgccgtgt tgtggggaag ttcaggggtc tgtgcgcaat acatcatgga
    16561 gcaaagccag atgtcatcgc agtttttgac tatgacgcgt ttgatattcg ccggtttgat
    16621 tctgctgacg ctgtcatttg ttcatggcga taaaatcttt tctattatta acaatcataa
    16681 agatgccatt agtctgctga ttttttccgt ggttggcgcg ctaaccgtac agctcacttt
    16741 tttgctaacc atcgaaaaat cgaatgctgc cacggcaacg gtgctgcaat tcctctcacc
    16801 gacgattatc gtcgcctggt tctcactggt gcgtaaatcc cgcccgggca ttctggtttt
    16861 ctgcgctatt ttgacatcgc tggtcggaac ttttttattg gtgacacacg gtaatccgac
    16921 gtcattatcg atctctcctt ccgcgttgtt ctggggcatt gcctcggcat ttgctgctgc
    16981 attctatacc acctatccct caacgctgat tgcccgctat ggcacgttac cagtcgtcgg
    17041 ctggagtatg ctgattggcg gtctgattct gttgcctttt tatgccagac aagggacaaa
    17101 ctttgtcgtt aacggcagtt tgattctggc gtttttttat ttggtggtca ttggtacatc
    17161 tctgacattt agcctgtacc tgaaaggagc acaattaatt ggcggtccaa aagccagcat
    17221 tttgagctgt gcagaaccat taagtagcgc gctactctct ttgctgttgc tggggatcac
    17281 gttcacatta ccggactggc tgggaacgct gctgattctg tcatcggtaa ttttgatttc
    17341 aatggattcc cgtcgccgcg ccagaaaaat aaatcgtccg gcgcgggatg agtgagaatt
    17401 accagccagg caccgcgcca ccgttaaaga tggtttctgc cgcttta
//
LOCUS       DAZGCQ010000099        17441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000099 DAZGCQ010000000
VERSION     DAZGCQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.099"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..1369
                     /gene="mhpT"
                     /locus_tag="ACHK07_003809"
     CDS             158..1369
                     /gene="mhpT"
                     /locus_tag="ACHK07_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO8104089.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            1471..2010
                     /locus_tag="ACHK07_003810"
     CDS             1471..2010
                     /locus_tag="ACHK07_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO8104090.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHK07_003811"
     CDS             complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHK07_003811"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8104091.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHK07_003812"
     CDS             complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHK07_003812"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO8104092.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHK07_003813"
     CDS             complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHK07_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO8104093.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHK07_003814"
     CDS             complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHK07_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO8104094.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(5799..7877)
                     /locus_tag="ACHK07_003815"
     CDS             complement(5799..7877)
                     /locus_tag="ACHK07_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8104095.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(7946..8977)
                     /locus_tag="ACHK07_003816"
     CDS             complement(7946..8977)
                     /locus_tag="ACHK07_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8104096.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHK07_003817"
     CDS             complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHK07_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8104097.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(10363..11904)
                     /locus_tag="ACHK07_003818"
     CDS             complement(10363..11904)
                     /locus_tag="ACHK07_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8104098.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQIVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMHERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(11904..12533)
                     /locus_tag="ACHK07_003819"
     CDS             complement(11904..12533)
                     /locus_tag="ACHK07_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO8104099.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRVQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            12836..13798
                     /gene="tauA"
                     /locus_tag="ACHK07_003820"
     CDS             12836..13798
                     /gene="tauA"
                     /locus_tag="ACHK07_003820"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8104100.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13811..14578
                     /gene="tauB"
                     /locus_tag="ACHK07_003821"
     CDS             13811..14578
                     /gene="tauB"
                     /locus_tag="ACHK07_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO8104101.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            14575..15402
                     /gene="tauC"
                     /locus_tag="ACHK07_003822"
     CDS             14575..15402
                     /gene="tauC"
                     /locus_tag="ACHK07_003822"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO8104102.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            15399..16250
                     /gene="tauD"
                     /locus_tag="ACHK07_003823"
     CDS             15399..16250
                     /gene="tauD"
                     /locus_tag="ACHK07_003823"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO8104103.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHK07_003824"
     CDS             complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHK07_003824"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO8104104.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         4139 a         4641 c         4755 g         3906 t
ORIGIN      
        1 aagcgtcgca tcaggcatct gcacacgact gccggatgcg gcgtgaacgc cttatccggc
       61 ctacatttcg cccgtaggca gtcattaaat agttctgatt acgcggctgg caatgtatca
      121 gtcgcgattc acctgcactc gcaacgaggt tcatcttatg tcgactcgta ccccttcatc
      181 atcttcatcc cgcctgatgc tgaccatcgg gctttgtttt ttggtcgctc tgatggaagg
      241 gctggatctt caggcggctg gcattgcggc gggtggcatc gcccaggctt tcgcactcga
      301 taaaatgcaa atgggctgga tattcagcgc cggaatactc ggtttactac ccggcgcgtt
      361 ggttggcgga atgctggcgg accgttatgg tcgcaagcgc attttgattg gctcagttgc
      421 gctgtttggt ttgttctcgc tggcaacggc gattgcctgg gatttcccct cactggtctt
      481 tgcgcggctg atgaccggtg tcgggctggg ggcggcgttg ccgaatctta tcgccctgac
      541 gtctgaagcc gcgggtccac gttttcgtgg gacggcagtg agcctgatgt attgcggtgt
      601 tcccattggc gcggcgctgg cggcgacact gggtttcgcg ggggcaaact tagcatggca
      661 aacggtgttt tgggtaggtg gtgtggtgcc gttgattctg gtgccgctat taatgcgctg
      721 gttgccggag tcggcggttt tcgctggcga aaaacaggct gcgccaccac tgcgtgcgtt
      781 atttgcgcca gaaacggcaa ccgcgacgct gctgctgtgg ctgtgttatt tcttcactct
      841 gctggtggtt tacatgttga tcaactggct accgctgctt ttggtggagc aaggattcca
      901 gccatcgcag gcggcagggg tgatgtttgc actgcaaatg ggtgcggcga gcgggacgtt
      961 aatgttgggc gcattgatgg ataagctgcg tccagtaacc atgtcgctac tgatttatag
     1021 cggcatgtta acttcgctgc tggcgcttgg aacggtgtcg tcatttaacg gtatgttgct
     1081 ggccggattt gtcgcggggt tgtttgcgac aggtgggcaa agcgttttgt atgccctggc
     1141 accgttgttt tacagttcgc agatccgcgc aacaggtgtg ggaacagccg tggcggtagg
     1201 gcgtctgggg gctatgagcg gtccgttact ggccgggaaa atgctggcat taggcactgg
     1261 cacggtcggc gtaatggccg cttctgcacc gggtattctt gttgctgggt tggcggtttt
     1321 tattttgatg agtcggagat caagaatgca gccgtgtgcc gatgcctgac gcgccttatt
     1381 aagcaggggc gaaagggact tgcccccaat acacccacgg cgtttacagg tatactcgct
     1441 aaaaattatt cagcgggttt ggaaacaaag atggcaaaac ttaccttaca agagcagttg
     1501 ctcaaagcag gattagtcac cagcaaaaaa gcggcgaagg tcgagagaac ggcgaaaaaa
     1561 tcgcgcgttc aggcgcgtga agctcgggcg gcggtagaag aaaataaaaa ggcacagctt
     1621 gagcgtgata aacagcttag cgaacagcaa aaacaagcgg cgttggcaaa agaatataaa
     1681 gctcaggtga agcagcttat tgaaatgaac cggatcacca ttgccaatgg cgatattggt
     1741 tttaacttca ctgatggcaa tctgattaag aagatttttg tcgataagct cacacaagcg
     1801 cagttgatta atggtcgtct ggcgattgcc cgcttgttgg tcgataacaa tagcgaaggt
     1861 gaatacgcca ttattcccgc cagcgttgcc gataaaattg ctcagcgtga tgccagcagt
     1921 attgtgttgc acagcgcgct cagtgccgaa gagcaggatg aagacgatcc gtatgccgac
     1981 ttcaaagtgc ctgatgattt gatgtggtaa acatagataa gccgtgcgca aatgcccgat
     2041 gcgacgctat gcgcgtctta tcatgcctat ggtttatggg cgaagtgtag gccggataag
     2101 gcgttcacgc cgcatccggc agccgtgcgc agatgcctga tgcgacgctt acgcgtctta
     2161 tcatgcctac ggtttatggg cgaagtgtag gccggataag gcgttcacgc cgcatccggc
     2221 agtcgtgcac tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc
     2281 aataaaactg gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt
     2341 cttgatattc atctcctggc agatcttttc aagatttgga gtccgcagct gttctgcgta
     2401 aaaatcatca ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat
     2461 aagactcacc ggatcgtaat ccaaccaggc atctttattt tcaccaagat atgcggcaaa
     2521 ggcttgctgt ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct
     2581 gacatattca tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg
     2641 accagagata gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc
     2701 gttgcggata tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag
     2761 gtaaaacccg gcaccttgcc cgagatcgta acggtcagca tctgcgacat gactgcctcg
     2821 cggactggtg tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcatccc
     2881 cgatttagta atgaaattct gctcgttgca ggtcaggcct gaaagccagt acagcaccgg
     2941 caatttttca ttcgcggctt ttggtgggag atagacgccg acattcattt cacatttcag
     3001 tgatgcggaa taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat
     3061 gagttccatc agtcattatc tcacggttaa tcccccagac ggctgcccgg ggagagtatt
     3121 aagaaccccg tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt
     3181 cgaatggatt tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg
     3241 gtatgcgtga caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc
     3301 ggtaactggg aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg
     3361 accaactgga atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc
     3421 gactgacccc agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg
     3481 atgcattcaa aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc
     3541 ggtttgtcgt agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg
     3601 aatttcttcg ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc
     3661 tgaaccactg ccagaccaat cgcgccaaga ccaaatacgg caacagaatc acctggctgg
     3721 actttagctg tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag
     3781 acgtgttcat ggtttgcttc tggattaatt ttggccagag acacttccgc gaccacggtg
     3841 tattcactga atgtagagca ccccatgtag tgataaagcg gctgcccgtt gtaagaaaaa
     3901 cgggtggtgc cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg
     3961 ttagttttgc cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc
     4021 acatggtcgc ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc
     4081 ccttcgtgac cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat
     4141 gcgtcggtat ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt
     4201 ggtgcaacgt caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca
     4261 cgtgatttca tctctcgctc ttcctcaata tagtaataga ttcagctatt taagataggc
     4321 acgaaccagt tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt
     4381 tcggtcaaac gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc
     4441 gccccgaacg gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga
     4501 ccgttccaga gcatcaatct gcccccgaat acgacgaact cgagtaagga cctttttctt
     4561 ctcttccgga gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg
     4621 gtatgctata tgtcaatgca taccccccta tagtatataa cactggagaa taattgttgt
     4681 ccgaagccta accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt
     4741 ttatgccgta tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc
     4801 accaatatcg ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca
     4861 ctctacggtc acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg
     4921 actctcttcg ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct
     4981 accttgcgcc gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc
     5041 ttcgctaaag ttcgccggga acaggttggc gtcgcccata aaacttgcca taaatcggga
     5101 ggctggctga cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat
     5161 taccagcaca gtgtcggaca ccgcgaaagc ttcactttga tcgtgagtga cgtagagcga
     5221 cgtgatatta aactgctttt gcagctcgcg gatcttatcg cgcatactgc ggcgcaggtt
     5281 ggcatcgagg ttacttaacg gctcatcaaa tagcagcacc tttggcttga ggatcaacgc
     5341 gcgggccagt gccacacgct gttgctgacc accggaaatc tgatcgacat agcggtcctc
     5401 gaagccttcc agatccacca ttgccagcgc ctccttcacc cgctgtttca cttcgctacg
     5461 cgacacgcca agcatcttca gcccgtagcc aacgttttca cccagcgaca tatgcgggaa
     5521 cagagcgtat gactgaaaca ccatgcagat atcgcgctgt tgaatggaac gatgcgtgac
     5581 atcttcgcca tcaataaaaa tttgcccttc gctcggtttt tccagcccgg caaccagacg
     5641 caaaatggtg gttttgccac agccggaagg accgagcagc gtcaccattt gcccttgtgg
     5701 aatggtgaga ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg
     5761 cagttcaaca aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta
     5821 gaacgggaga tacgagcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc
     5881 accacgatca gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg
     5941 ttaagaatgt aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg
     6001 accgtcgtga tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc
     6061 agcggcagta aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct
     6121 tcatccagcg atttgtcgat ctgccccaga cccgcaatcc cggcgcgaat acccaccgga
     6181 acgttacgca tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga
     6241 gcgctgttaa aggcgagaat atacgagacg cccgccaccg tacccggtac ggcaaagcac
     6301 agcatggtgg tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag
     6361 gcgatgagca ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta
     6421 tccagcagtg aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta
     6481 tccagcgtca gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg
     6541 ccgtagagca gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc
     6601 agggtgaccg gtagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa
     6661 gagcgtttac caatccacat atactggatg cagaacacca gcagcgagaa cagcagcagg
     6721 aaagcgccaa gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa
     6781 atttgcgtcg ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc
     6841 agtgactgta ccacgacgat taaaaaggca ttggcgagtg cgggcttcaa caacggcaca
     6901 aacacgccgt taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc
     6961 gagggatgaa tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc
     7021 agaacctgtg ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca
     7081 aaccactcca ccataaattc cgtaacatag ccggagcgcc ccatcatgag cgtcacgcct
     7141 aagccgacca caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg gccaataatg
     7201 gcgctgcgtt tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagtacag
     7261 cctatcccca ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc
     7321 tgcgagagca cggtcataaa cgccagcggg gcgaactctc ccgcgtcgtt ggtgaacatc
     7381 gggataaaaa tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca
     7441 atcagtgagc cgatcacgaa gcggtcgcca cccagccatt cgaggcgggt taaggccagc
     7501 gtcataatcg cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca
     7561 accacggtgg cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa
     7621 taatgacggc tacgttgttc gcgcgtggcc tcactaaacg ggcggaccag caacaggctc
     7681 gggagtaaat accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc
     7741 tcatcactcg tggactccag caggccataa tcgaggctcc agctcggcaa caggacaaat
     7801 gccagccaac ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt
     7861 gcaagtgtct gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca
     7921 cccggcaaat gcgcctaatg cgggtttatt tacccatttt tacttcgcta acccatttat
     7981 taatcaatgc cttacgaaca tccgtggaac cgtatttgtc catgtcgtag ttgattaatt
     8041 tcaggtcgtc gagcttcagt gagtttggcg acgtttcggc ggtggtattg gtcaggatct
     8101 gataagactt ccctttcttc cacgccagtt cctgcgcttc ttttgataat acccagtcca
     8161 caaacagttt ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt
     8221 cgtagcctgt accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca
     8281 gcgagtagtc atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg
     8341 ccggggctat gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat
     8401 caaaggcctg atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac
     8461 cggaactttg cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt
     8521 tccagcactt cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac
     8581 cgaggatccc cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga
     8641 atttctccat gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc
     8701 cagcctgcga ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct
     8761 tttcagcatc gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta
     8821 catcatattt ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc
     8881 aatagaccac cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg
     8941 cagaagcaat cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt
     9001 ggtggtgatg cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac
     9061 cgagagtaaa ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca
     9121 gcctgccagc gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc
     9181 cgcagctttg tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac
     9241 gaaaaagccc acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag
     9301 ccacagtgcg gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg
     9361 ttgcccgcta aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc
     9421 cacttcaaac agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt
     9481 cagccagata ttgccccagt cgttaagcgc aatccggatc acataaacca gtacataaga
     9541 gacgcccagt agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag
     9601 ccccatcggc ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc
     9661 aactgtcggt aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc
     9721 taatgccatg ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc
     9781 ggcaaaggcg ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca
     9841 gccccaccag aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg
     9901 tggccatccc cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag
     9961 tgattcgcca aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat
    10021 aaaaccgcgt tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta
    10081 gctgagataa aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt
    10141 ttgtagcgcc ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc
    10201 aataatcatg cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct
    10261 cgctgaacgc gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa
    10321 ggggatgaga gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg
    10381 caaaaatgtg ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc
    10441 gccgcccagg gcgctgacgc gctcatgcat cccctgaatg ccaaatccgg ccattttccc
    10501 gctggcgatc cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg
    10561 caggataatc gtcacttcgc tggcttcggc gtgtttacag atgttattaa gcaactcctg
    10621 caataaccga tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata
    10681 ggcaaactgg caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcaacaa
    10741 cgcttcgcga aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg
    10801 ggtggagaga tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg
    10861 cgccggatca cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg
    10921 accgatttcg tcatgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat
    10981 cagcttttcc gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg
    11041 gtgcaggttt agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag
    11101 caaggcctgg gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat
    11161 ctggcgggcg atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca
    11221 gccaaatttc cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc
    11281 aatttcaggt gacagcacta tctgagtgcc gataccaata ataaaaaaca ggctacacca
    11341 gatgattaaa gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg
    11401 atgctgctgg cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc
    11461 ggtaaatgag gtcaaacccg gcagcattat tgggcctgac ataaaagggg agagcagggc
    11521 ggtattcaac aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta
    11581 aagtggaaaa cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg
    11641 cgttgccagc atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag
    11701 caaagggggg agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag
    11761 ataaactccc tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct
    11821 gagcgtccac agcatcagcc agccggttcc ccaggccagc acaataaaca gcgaaatcac
    11881 ccaatgacga agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa
    11941 tgtaccagtt caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca
    12001 tgtaccgttt tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg
    12061 acaagtaaat caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt
    12121 tgcccgccgc cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg
    12181 accgagcgca ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg
    12241 gcaccggcat ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg
    12301 gcgcgaaatt gcggtttttg tacacgcagg cgcttaagca aactgaggcc gttttcgtcc
    12361 ggcatggcaa tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc
    12421 tgcgcggcac tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt
    12481 gcgaagccag agcgcaccac cacatggtca tccaccagca ctacccgaat catacttttc
    12541 ttctcttatt atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc
    12601 gacagatatg gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat
    12661 ctggcttgtg aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac
    12721 cattgccgat ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc
    12781 aaactgaccc gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc
    12841 aatttcatcg cgtaacacac ttcttgccgc actggcattc atcgcttttc aggcacaggc
    12901 ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga
    12961 caacaccttt gctaaagaaa gcggagcaac cgtggactgg cgtaagtttg acagcggagc
    13021 cagcatcgtg cgggcgctgg cttcaggcga cgtgcaaatc ggcaacctcg gttccagccc
    13081 attagcggtt gcagccagcc aacaggtgcc gattgaagtc ttcttgctag cgtcaaaact
    13141 gggtaactcc gaagcactgg tggtaaagaa aactatcagc aaaccggaag atctgattgg
    13201 caaacgcatc gccgtaccgt ttatctccac tacccactac agcctgctgg cggcgctgaa
    13261 gcactggggt attaaacctg ggcaagtgga gatagttaac ctgcagccgc ccgcgattat
    13321 cgctgcatgg cagcggggag acattgatgg tgcttatgtc tgggcaccgg cggttaacgc
    13381 cctggaaaaa gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc
    13441 aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa
    13501 agcgttcgct aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg
    13561 gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga
    13621 cgttccgggg ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact
    13681 gaccggaccg gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa
    13741 ggtcccggct gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa
    13801 ggaggcgcag atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggcact
    13861 ggaagatatc aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg
    13921 ctgcggtaaa accaccctgc tgaatctgat tgccggtttt gtgccttatc agcatggcag
    13981 cattcaactg gcgggtaagc gtattgaggg accgggagca gagcgtggcg tagtttttca
    14041 gaatgaaggg ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt
    14101 ggcaggtata gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg
    14161 gctggaaggc gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt
    14221 gggcattgcc cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg
    14281 tgcgctggac gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga
    14341 gacgggcaag caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac
    14401 tgaactggtt ctgctttcat ccggccctgg acgtgtgctg gagcggctgc cgctcaactt
    14461 tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat
    14521 cgccatgcgc gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt
    14581 gtgctcatta atgaaaaact gcattcgcgg cggttgaaat ggcgctggcc gctctcgcgt
    14641 caggtgacct taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg
    14701 gcgctgcaac tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg
    14761 ctcaccattg ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc
    14821 agcctgacgc gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg
    14881 atcgccatgg ggctaagccc gacggtgcgc ggcattctgg atccggtaat cgaactttat
    14941 cgtccggtgc cgccgctggc ttatttacca ctgatggtga tctggtttgg tattggcgaa
    15001 acctcgaaga tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg
    15061 gcgggggtga aaagcgcgca gcaggttcgc attcgtgccg cccagtcgct gggtgccagc
    15121 cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga
    15181 ttacgcattg gtctgggggt gggctggtct accctggtag cggcggagct gattgccgcg
    15241 acgcgcggtt taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg
    15301 ctggcgggga tcgcggtgat tgcgattatc gcctttcttt tagaactggg tttgcgcgcg
    15361 ttacagcgcc gactgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta
    15421 ccccgctggg gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa
    15481 gcgataatca gtttgaacag ctttaccatg cggtgctgcg ccatcaggtg gtgtttctgc
    15541 gcgatcaagc tattacgccg ctgcagcaac gcgcgctggc ccagcgtttt ggcgaactgc
    15601 atattcaccc tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc
    15661 ataacgataa tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc
    15721 cacccgcagg ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct
    15781 ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg
    15841 ggctgcgtgc ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg
    15901 aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa tccgccgttg ctacatccgg
    15961 tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta
    16021 cgcgaattgt tgatgtgagc gagaaagaga gcgaagcctt gttaagtttt ttgtttgccc
    16081 atatcaccaa accggagttt caggtgcgct ggcgctggca accaaatgat attgcgattt
    16141 gggataaccg cgtgacccag cactatgcca atgccgatta cctgccacag cgacggatta
    16201 tgcatcgggc gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg
    16261 gacggtcccc tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtactttc
    16321 aacaggttaa ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc
    16381 caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa
    16441 gctttcgagc accactttct cttcatctat agcacccgcc agcgcggcga acttaatcat
    16501 cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc
    16561 acgcacgatg tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc
    16621 ttcatccagc agtgattcac gaatcgcctc gcgacggttc attgggttca tctgatagct
    16681 tttgcggtcg ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga
    16741 ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc
    16801 ctggcgaatc gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc
    16861 tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac
    16921 gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc
    16981 tgacatgaca atcatttctg gcactgtctg cttgcagatg cgcgacatac gcgccaccag
    17041 tccatcttcc cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccaaaagt
    17101 catcacggaa cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc
    17161 tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc
    17221 aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg
    17281 cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aaatctgtca tggtctgcct
    17341 gatgtttgtg gaatcgaggg gcatagtata cctgaagcag ggtagggatg ttttacgaaa
    17401 gttgtcgcga tgttgacaag aagagaatgg aagagaggcc a
//
LOCUS       DAZGCQ010000100        17096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000100 DAZGCQ010000000
VERSION     DAZGCQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.100"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1410)
                     /locus_tag="ACHK07_003825"
     CDS             complement(<1..1410)
                     /locus_tag="ACHK07_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8104105.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNVGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNN"
     gene            complement(1783..2655)
                     /locus_tag="ACHK07_003826"
     CDS             complement(1783..2655)
                     /locus_tag="ACHK07_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8104106.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHTGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            3885..5369
                     /locus_tag="ACHK07_003827"
     CDS             3885..5369
                     /locus_tag="ACHK07_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8104107.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PNESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(5691..6293)
                     /locus_tag="ACHK07_003828"
     CDS             complement(5691..6293)
                     /locus_tag="ACHK07_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104108.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLMYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(6380..6586)
                     /locus_tag="ACHK07_003829"
     CDS             complement(6380..6586)
                     /locus_tag="ACHK07_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8104109.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            7345..7494
                     /locus_tag="ACHK07_003830"
     CDS             7345..7494
                     /locus_tag="ACHK07_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO8104110.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(7909..8109)
                     /locus_tag="ACHK07_003831"
     CDS             complement(7909..8109)
                     /locus_tag="ACHK07_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104111.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            8314..8883
                     /locus_tag="ACHK07_003832"
     CDS             8314..8883
                     /locus_tag="ACHK07_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8104112.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGIIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            9144..9521
                     /locus_tag="ACHK07_003833"
                     /pseudo
     CDS             9144..9521
                     /locus_tag="ACHK07_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            9525..9950
                     /locus_tag="ACHK07_003834"
     CDS             9525..9950
                     /locus_tag="ACHK07_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8104113.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLSVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            10197..10394
                     /locus_tag="ACHK07_003835"
     CDS             10197..10394
                     /locus_tag="ACHK07_003835"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8104114.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            10422..10988
                     /locus_tag="ACHK07_003836"
     CDS             10422..10988
                     /locus_tag="ACHK07_003836"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8104115.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESYGAIERHLGCSDF"
     gene            11044..11308
                     /locus_tag="ACHK07_003837"
                     /pseudo
     CDS             11044..11308
                     /locus_tag="ACHK07_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11374..11571
                     /locus_tag="ACHK07_003838"
     CDS             11374..11571
                     /locus_tag="ACHK07_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8104116.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            12300..13424
                     /locus_tag="ACHK07_003839"
     CDS             12300..13424
                     /locus_tag="ACHK07_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO8104117.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIHFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(13566..14840)
                     /locus_tag="ACHK07_003840"
     CDS             complement(13566..14840)
                     /locus_tag="ACHK07_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO8104118.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(15379..15813)
                     /gene="mchS3"
                     /locus_tag="ACHK07_003841"
     CDS             complement(15379..15813)
                     /gene="mchS3"
                     /locus_tag="ACHK07_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchS3 family protein"
                     /protein_id="HFO8104119.1"
                     /translation="MYLTKKIIISMMFILPSAAFSSDPPPLQQSLEKTTYFSIGMNGF
                     IGYQSEGEKLYTHILTLDNPEEIFKNIIKNRKSTKESKIYAACGLYYLNVENIESLFN
                     ENDKQEYVSVLRGDILTKIKLNDILNSVIINGCNTKLISEHK"
     gene            complement(15969..16214)
                     /locus_tag="ACHK07_003842"
     CDS             complement(15969..16214)
                     /locus_tag="ACHK07_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104120.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
     gene            complement(16282..16518)
                     /locus_tag="ACHK07_003843"
     CDS             complement(16282..16518)
                     /locus_tag="ACHK07_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104121.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
     gene            16627..16836
                     /gene="mchI"
                     /locus_tag="ACHK07_003844"
     CDS             16627..16836
                     /gene="mchI"
                     /locus_tag="ACHK07_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO8104122.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            16864..17091
                     /gene="mchB"
                     /locus_tag="ACHK07_003845"
     CDS             16864..17091
                     /gene="mchB"
                     /locus_tag="ACHK07_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO8104123.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
BASE COUNT         4373 a         3799 c         4150 g         4774 t
ORIGIN      
        1 gttgttcacc gtcttcccgg aaagggtaag tatggcgccg ttattcagcg tggtggtgcc
       61 cgccagtgtg ccgcccctgg cggtgaacag tccgccattt accgtggtat ccgtggccgt
      121 atcaccgacg ttcagcgtga atgaactgcc tttttccagc atcagggcat ccgcctgacc
      181 gcctccgata ctgaatgcct ttccgtcgct ccgtgtacca ctgacagcgg caccggaatc
      241 ggccagcagt acaccgccat ttcccatgga tacggtggtg gcggtaccac cgttgcggac
      301 atccagcgtt ccgccatcat ccacgcgggt attagtggct gtgtgtccgg tcagcacatc
      361 caggcgtccg ccattttcca gtacgacatt atcagcttta ccctccacaa ccgagaatgc
      421 tccaaggcgg tttatgccgg taacggttgc agccgtactg gtaaccagtg caccgcccgt
      481 gttctgggtg actgcagtgg cttccccgcc ggcatgtacc ctcagttcac cgttctgatt
      541 tatggtggtg ttaccgacag caccacctgc cttaacaacc tgccagcccc cctcgttaat
      601 taccgtgttc agcgcaagtc cgtccttgtg cacatactgg taaccgccat tcagtgtggt
      661 attcagggcc cttccgtgta ccgactggtc accacctgcg taaatgagag tgtcacgggc
      721 tatcccggaa aataagataa tctgccgtcc atttttgttg atggtggtgg attctgctgt
      781 tccctggacc ttctgacccg tgtacgagtt gtcagaatcc gggccgcctt ctgcgccggt
      841 gttgatgatg gttcctgttg ccagcccgcc acttttaacg ctctggtaac cgtcgcggtt
      901 gataattgta ccggtggcaa ccccgccctc atgaacccac tgctcgcctc tgttattcag
      961 cgtggtgttc accgccagcc cgttcaggct ctgtcccccg ccgtcacgaa taaccgtatc
     1021 actggctgtt cccccctcca gcacgacctg acgaccattt gtggtaacag tggtgtttcc
     1081 ggctatcccg ccattctgta tccattgccc accggtgttt tcttcactgt ccggccccag
     1141 ttccagcccg gtactgatgg tcattccgtt ggccgtaccg aggacaatct ggttgtcatg
     1201 atttgtcagt gttccgtcgt tcacggtttc tcccgcctgt acaaccgtgt cagcagccag
     1261 tgccgggact gatgtgacag cagcaagaga cagcgcaacc gccacaccgg cgcgttttcc
     1321 ccgtgagcgg gccagttcgg aggccaccac cagggtgccc gtaatgtgat tccataccag
     1381 cctgtagctg gtgttcagat gtcgtttcat cagcttttcc ttacagaggg tgaataaaaa
     1441 agccggtacc cacaaatgtg gataccggca ggtacaaaac agcatggtca gataaagtgg
     1501 tttttcccgg acataaatgc ttcccctctc tcatccgcca gagaagtatc agtccgttgt
     1561 gtgcaattcc gttctgcatg atgaagagat tcagctgcct tttcgcagac cattaaccac
     1621 tataaacgat cgatagtaac gatcgatacc tgttttatcg atcgttttat tctattgaca
     1681 gcagagcggt cacgcaagag aaacaggagg gggatgaggg gtttgtgtca tgttttcagt
     1741 tggttaacgc aatgtaatca ggcatttccc ctggcagagt gttcagaaga aaatccagtt
     1801 ccatacagcg cgggcaacag agaccaccga gtcacggacg gcacgcagaa ccgtgcgtgc
     1861 aacagaggca atacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa
     1921 ctgttcacgg gcctgagacc ggacagattc cgtgcgcagc tcgtcctgca gtcgggtcat
     1981 caggggactg gcggcatggt cgggaagcgc tgtcatgagc gcactgacca gcgtatccag
     2041 ttcccagccg gtgcgggccg atacggccac aaccggatgt acgggccgga acagacggaa
     2101 taccgcctcc gttttttcgc gaatattctg tgcctgtgca ggagaaggct gaataccggc
     2161 catatcccat tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagtacccg
     2221 ctgatgtccc cggtgcagga tgtgtcgcca gaaatactca tccacagaca gggcacggtc
     2281 atcggcttta atcagccaca gtaccaggtc cagttcaggc agaatgtcac ggtacagggc
     2341 ctcatactct gcatccctgt cccggctctc gcccaccccg ggcaggtcag tgataaccat
     2401 gctgtgaccg tggccactca gacggaagcg ccgtacttcc cgggtgccgg tgtgaacatc
     2461 actgaccggg gtgacctccc cctgaaacag tgcattacag agtgaggatt taccggcccc
     2521 gcttttaccc ataatgccaa tcactggttc gtgacgggtg agtttgcgca gatgttccag
     2581 gatgtgacgg gaaagcgagt aaggcaggga ggagagcggt ttttcaattg cctcaatggc
     2641 atcagacgga ttcatacatg tattcccggt gaaaaaagac aaaaccccgc gcaccggaaa
     2701 ggtggcgggg gattcacgga gataatatct gtctgaaatt gttttgaatc gatgggattg
     2761 ttgcgtatta acatgaagga gatcaggact tatccgttac tgggtgatct ccttccatga
     2821 aaatacgccg ttattgttaa acagcaggct gatttctccg taaaggggaa aatatttatc
     2881 ggctgcatga ttatcaggtg tgtctgtgtt tactgctgtg agtatttttc agtgtattgt
     2941 gggggagatg tcagtgggag atattaaatt tacgtttata attatcttac ttcttctgtg
     3001 tgttttttat aagagtgcac acgtaatgat atctgttgat accatgtggt taagtgtttc
     3061 ccttcctggt catgcgtaat gattaaatgc ttttgtgaca ccattcttcc ggtctgaata
     3121 tttatccagt tcttttttat ttgtgcagtg atactgtcgg aagagaattc agtaatgtga
     3181 ggtgtcctgg tcaggataat atctgctgag atattattag ttgagtgtgt ttttactgag
     3241 caaaagctgg cttttaattc ctgtactgaa acgctgtcgc tcgtccgggg ttaactgaaa
     3301 ctggatgatg atgtaaaatg aaggctgttt ttgtgtttgt cgaagaagtg ttattttaat
     3361 ccattctcat cattggctgg cgattataca gaagtatttg aagaaaaaat ggtgcatttt
     3421 atctgcgtca gcagggagga agtattttat tgatgtatca gtattaaatg gtttttgtta
     3481 tttataaata tgtcatcctg tccatagaat attgtactca gcattcagtg caggaactgt
     3541 agtcttcttt attttattct tcttcaggag ggggggattc ctgaaggcgt tattcccggt
     3601 gttctgttta tgattcttcc cggcaggaaa ccggtatctg caatacccgg gatgatgctt
     3661 tttgttcgta cgtggttgtg taaaatttga atagtggatg tgctctgaag ggaaggaggg
     3721 gacacacaga ttcattttgt ctttcttcag cagcaaaaca cactgtggtt attatttaac
     3781 cattaaagca acaaaaattt ttacctcata ttgatttgac tgctattttt gctattaata
     3841 gccagaattg cagtactttt atatctgtgt gtagtgaggt tactatgtca ttcggtcagc
     3901 cttgtgatga gtttcccctg agttcgctgc ctccactcat tcgtgatgcc gttattgaag
     3961 ctcagcaaat tacgcaggct ccccttgggc ttgtggcagc gtctgccctg ggcgctgttt
     4021 cacttgtctg ccagaatctg attgatgtct gcagactgaa tacactgcgg gggccggtct
     4081 ctcttttttt cctgacgctt gccgagtccg gagaacgaaa aaccgcagtg gacaaattat
     4141 taatgaagcc cctgtaccag caggagatgc agttatattc cagatacaaa agtgaactgg
     4201 ctgtctggaa aaacaaggaa gagcttttaa aggctcagaa aaaagcattg ttgtcaaaac
     4261 tgaataaaga actccgcaaa ggcgcggatg agtcagaaac cctcagacaa ctggaagtac
     4321 ttcagaaaaa cagtgcggaa gaacctgtga ggtataaatt catttttaat gatgccacga
     4381 ctgcggcaat taagaatcag ctttgtggta aatggcgttc agtgggtatt atgtcagatg
     4441 aagccggtat tatatttgat ggatataccc tgtctgagtt gccttttatt aacaaaatgt
     4501 gggatggctc tgtattgtct gtggacagaa agaatgaacc cgaacagatg attgaaaatg
     4561 ccagaatgac gttatccctc atggtgcaac ccggtctttt tgaccgatat atggagcgaa
     4621 aaggttctgt tgccagggac agtggttttc ttgcccgttg tcttatcagt aaacctgcaa
     4681 ccacgcaggg gaaacgcttt attaatggag ctgtgattcc gggaggatca ttaacggcat
     4741 ttcatgaacg attaatggaa ctggccagag gcagtattga aaaatccagt gaggatgaac
     4801 ggtattgtct gcatttttcg cctgaagcgc aaaaaatatt tattgagcac tataatgttc
     4861 tggagcagga tttatcccct tccggaccct taagcccgtt tcggggacat gtttcaaaaa
     4921 aaacagaaaa cattgcaaga attgcagcgt tatttcagta tttcagctat ggtgaaggga
     4981 aaatctctgc cgatatcatg acgtctgctg tcgtaataag ttcgtggtac acagatgaat
     5041 ataaaaaatt atttgcgtta cctaatgaat ctgaactgca gcagaaggac gccgaggagc
     5101 tgttcgactg gctcattgag gagtgccgcg gagaatgccc gccccgtgtc aggaaaaatt
     5161 atattctcca gtgtggcccg ggccggttca gaaacaggaa aaaactgaac gcattgctga
     5221 acattcttga atctcagttc aggctttctg tcgtgccgga aggaaaaacc atgtatgttc
     5281 tgcttcccca gatagccagt ttaaagttgt ctgatgtctc tggtattttt acctcgggtt
     5341 atcactacaa taagctgaga gcgaagtaac cggtgagcag tttttctcca ttatgcttca
     5401 gacttagatg attgattatt atggggattt catcaccgat attcagatgt atgaatatcg
     5461 gtgatgaact aaagctcagg aactgcgtgg tggtgatgaa tcaacaggca tataactgat
     5521 acccgccggt cagaagttcc agcctgcgtc tttcctgtct gcgtgcccgc agtccggcgt
     5581 tcaggtgcag cgtgtgacag aacgcacgga gagcgaaacg gcaggcagac aggccgttca
     5641 gtcagccctg gcaccctgcc cggataccag cttccctgaa acaactggca tcaggtgcgc
     5701 ttatcgtcaa ccgttgtgcg gtatgccagg agcacgtact gatccatcag atccgtcagt
     5761 gctgcgctct gcttctgacg gttgatgatc cattcctcca gctctttttc tgatttccgt
     5821 ttcagattct ccgtccagtc gtcaaggcag tttccggtca ttaaatcaag ccagcaaagc
     5881 cagaccagtt tgaggcgcaa ctccgtgcca ccaaactgac ggaagaggct gatcagttta
     5941 tttgccagcg aacgctgcct cagacgttca cattcagaca ggagaatgcc ttccagcatc
     6001 ggatgatgaa ccagcagatg ttcactggtg cataaattca ctatcagatc atcatccagg
     6061 ggcggcgtgg tccgtttgtc cggaccatac atcagccggt gcagactctg gggtacgatg
     6121 gcaatctccg gtttttcacc attgaatgac acccacccgg gcgtcagatt atggaagtag
     6181 tagatattcg gatgacacac gccccaggat acggcgtgcc gggtgaagcc ggtcagccat
     6241 ttctggtagc tttcgtggag cagctcgtaa cgggtctgat actgtggttt cattttcata
     6301 ttcctcacgg gggataacag gcagcagaac gccgccggca ggccggagac tgcgtctgct
     6361 gcagatgaac ggttaagaat tacggacggg actgtgcaat acgcgcctgc agccaggctt
     6421 ccacttcgct tttcagccat cgggagctgc ggcccatttt gatgggcgca ggaaagtcac
     6481 catccttgat gagtttgtaa aaccacttat cggtcaggcc ggtcagttga gtgataaatg
     6541 ccatgtcgac catctggtca tccatcagtg aaactggggt gatcatgaga atgttcctcc
     6601 ggatgttgtt aaaccagccg gtgtggtcat gaaaacaacg caccaccttc accggcataa
     6661 caaccgggga gaaacgtaaa aaataatcgg gaatgtgacc gccaggagat aaacggggag
     6721 gttacggtgg ctgagccgta ccggtatgcc gggtggttgt tacgaaacga tgcttccgca
     6781 cgcgcgcgcg ataaagaagg gatgattttc gtgaggaaga ggggaaaact ggcgttctga
     6841 taattgcaga gttatttacg gggcggttac gatggggcag accgtgtccg gtgggcaggt
     6901 gacaggtatc accgatggga taagactgtc tgattggtat tataaatata taagagagat
     6961 caaacacatg agatcaaata catgagttca tatatgtgaa ataaacacgg tgagtgtgat
     7021 cagtgggcat gatgagaacg cagtagcaac agactgcgct atccctgata gctggcgtat
     7081 aacccgcgta tataaaacgt atctgcaatg tacgtgaaca gagtgcatta agcatccgtc
     7141 tgagcggtaa acatatccga cctggtgagt gacagtctga gagaccggtt aagtggtggc
     7201 tcgggagtaa tgagtttggg gtgggtacca gaatgccagt ctggcggtat gctgacagta
     7261 tcacgttaat tgcagcataa agtgatgaga ggctaatgga agttaaaacc aaagcagttc
     7321 cgtcgttgtt catcccggga gacgctggaa aaagtgattt cccacacgcg ttataaactt
     7381 acctctgcgg aactggaagc cttcaactct gtggttgatc actggctggc agaactgaca
     7441 atgaacaagc tttacgatcg tgttcccgct tcagtctgga aatatgtcat ctgagcgttc
     7501 ggtaatcaca actgcatcgc gccaggtgtt atccgctttg tgattcttat tggctcttct
     7561 caggttatta aaagctgaat atgttcctgt tgtcgggaac caggataata ccgggaacgt
     7621 taagcggtat agcgacaaca aagaattcga aatctaaagg gaatggtgcc ggaggcgtgg
     7681 gcggtaatgg gctgattttc aggtattacc ggcttacagc gtgtggttga tattgagggg
     7741 ctgtgagttt cacgtatttg tcagaaaccc agacgagttt tcctgtatga gacgaaataa
     7801 ggcagcggca atacctggaa aaatttaata tggtatgaag gagaacctgt cggttacgct
     7861 taatataccc ggtacactgt cagctcaggg gatacatgaa ccgttatctc accagctaca
     7921 cagtatatac acactgacgc tgacagtagt cagcaggacc acaataatca gtgccagcag
     7981 tatagtaacg atacggtctg tattccggta atagccgaag gggtcgacag aaccacaata
     8041 cgggcaacac acagtattat gagtaatcgc gtttccacag tcccagcact tctttttgcg
     8101 tatcaccata actccctcct cacagcctta tccgtaatcg gttttttaca ttaaaaaaac
     8161 aacgctgaaa attcaaccgt cagtccggag acgaccgttc gggttatctc agagtgcctg
     8221 agatattgcc tggttggaga tcacttcagg gactctgcgc attttttacg gcgtggttaa
     8281 tccgtatgaa cgaaaccata cggagattta accatgacct acaaatacaa ccccttctgg
     8341 cagcaacgta ttcgtgagac ggtgcggcac gcactggatg ttcatccccg tctgacggca
     8401 ttgcgggttg atctgcgtct cccggatgta ccggcagcaa cggacgcggc tgtgatatcc
     8461 cgcttcatca atgccctgaa agcccgaatc gacgcttacc agaaacgtaa gcatcgggaa
     8521 ggtaaacgcg tgcattcctc gaccctgcat tacgcctggg cccgggagtt cggggagctt
     8581 aaaggcaaaa aacactatca cctgctgctg ctggtcaacc gggatacctg gtgtcgtgcc
     8641 ggcgattacc gcgctccggg gtcactggcc gggattatta aacaggcctg gtgcagtgcc
     8701 ctgggagtgg atgccggacg ttatgacacg ctggcacatt ttcctgacaa gccagcggtg
     8761 tggctggaac gcgatgatga aaccggcttt cagcaggtgc tggaacgttc tggctatctg
     8821 gcgaaggagc ataccaaagc tcgcggcacc ggtgagcgca attttggctg tagccggagt
     8881 tgaacacgac aggcgcacca atacccggca aaccaaaagt atgggttgtt gtctggcggc
     8941 aacgatgtat ccactcgccc actgactgtt ttttacgtca gtgggccatt gtgtgacaac
     9001 cacagtgctg taacagcaac cagcctgctt tacccaaact ccagtccaga ttttaactga
     9061 accgccattc acaccctgac cacgctgccc gtacctggac ggctgttgcc tgcgtgtcgt
     9121 ctggcggctg aggaatatat actatgtacg caaaatcctt tctcgctctt gatggcaacg
     9181 gacgtctgac gggggcccgt accgcacaga ctgcgcctta cgaccgctac acctgccacc
     9241 tttgcggcag tgcactcaga taccacccgc aatacgacac tgaacggccc tggtttgaac
     9301 acgctgacga agggctgacg gagcacgcgc agcaatgtcc ttatgtcagg ccggaacgca
     9361 gagaaataca gttgattaaa cgtctgcagc aattcgtccc ggatgcctta cccgtggtgc
     9421 gtaaagccag ctggcactgc agacaatgtc accacgatta ttatggggag cggtactgca
     9481 cacactgcca gaccggacgc ttcagtgagg aggggggtag cagaatgaac ggtataaaaa
     9541 cggggctggg gatcacgccg ggtgaacaca ttatcagtgc cgactctgcg ctcagcagaa
     9601 atatccggca ttgtttctgc ctttcctgcc atggccggtt gattctgcag acagatgctc
     9661 agggggcatg gtttgagcat gatttacatg ccctgagtga gcaacagaag gcggactgtg
     9721 tggtgctgaa tccggaaaag tcccaccctt acattgagga tatggcgatg tttttatcgc
     9781 cattatcggt cgtgctggag tggcattgtg tgatgtgtga gcagtttttt catgggaaga
     9841 agttctgtga ggcctgtggt acggggattt attgcagagc ggtatgtacg aagtctgttt
     9901 acagctacca gccagacttg tttgaggact gcggaggtgc gagtacttaa tccgaaatcc
     9961 ccgctctgcg cagggcgttc agaggtattg ccaacctgta ttttatccgg tgtttcgcac
    10021 ccaccccgtt cgtgacacaa cggcgcgaca ccggatatcc gcagtcctgc ttacctgaat
    10081 aaaacccggg gtattgattg gtagtgtggt gcggaatcgt accgtgatct cgcatccgcc
    10141 gaattcgcac tgtggtgtca atcaaccata cgcgagagtc ctctccgtat ttatcagtgc
    10201 ctcaggatga tttctgcata gttaatccag ggggctgcat tgttcatttc cctgctaatg
    10261 gacgtgacat ccaggatatt aatgctcctg atttccctga gcagtatcag cgcttattgg
    10321 cacgtctgga ttatttgacg aagcccgatg ctaaagcatc aggccagcgt aactctggct
    10381 acagcggttt ttgacctgtt gtaactgtaa ggtgatttat tatgtataca gaatcaaatg
    10441 ttaagctttc ctgtaacgcc ctctggcaaa agtgtgtcct tgcaactctg tatcgggctg
    10501 cagccacaca tcctgaaatt aaattattac aatttaaatt aataccaggg cagggtatta
    10561 ataaggccat tccctctctg atggctgatt ttactggtga actgcataga ctcatttctg
    10621 aatatttgca gtgtaagcga cgtaaagatc aatctgttga gcccacccct ttatattatc
    10681 tctggagtag ggaagctgtg gctaacgata ccactcctta tcttatggtg ctgctactta
    10741 ataacgatgt ctggtgtaag agtcgtgagg tggtggttct aagaaatctg attgagcagg
    10801 cgtggggaaa agtattgagt gaagataact ccattacaca tctggttgtg tatcctgaag
    10861 tgttgtcgtt ggtatttgat gcagctaaca catacaacgc tgagcctggt gtactggaac
    10921 agacggcccg ggcttacatt gaatcctatg gtgccattga acgtcatctt gggtgtagtg
    10981 acttttaacg tcaggtgacg cattgtcact tcagacctgc tacctgtacg ccggaatata
    11041 atggtcagag aggcatattt cgacgcccct aatctggggc gtttgattca ggatgtctgg
    11101 aacaatccgg gctttgtgtc accgggcgca tcaaagctgt accacccggg atgtggcgct
    11161 ggatcgtcat ttaccggact gcgaagacat acttaaagag gtgatatggg cgttcagtga
    11221 ttttgtccgc gataatcgtg gtgtgtatga tccggaagcc cgttaccccg ccggtaatcc
    11281 ctggtatccc gtaacaggcc agttctgaaa gcacagctca gcagattccc cgcattcgca
    11341 caatcttcat aaatctcaca ggacattctg accatgttga ctacaacaag ccacgacagc
    11401 gtatttctgc gtgccgataa ttccctgatc gacatgaact atatcaccag tttcaccggt
    11461 atgaccgaca aatggtttta caagttgatc agtgaaggcc atttccctaa acccatcaaa
    11521 ctggggcgca gcagccgctg gtacaaaagt gaagtggagc agtggaagtg aagtagagca
    11581 gtggatgtag caacgaatcg aggaatcctg ggggcagcag catgaaacgt gttgtaatgc
    11641 cggtacgctg gcaatgtgca aaatgccagc gctggtattg tggaacccgg ccctgccctt
    11701 ggtgctggcg atattcccgt tcatcttcct gctgatacct tccgttcagc agcgggttgt
    11761 tatcccttcc tgactgattc gtcattccat tcttattgat tagaactggc attacaccgg
    11821 tgccggcgtg ctttcctgcg tgtctgcgcc ggttcagtaa attagaaaag aaacatttgt
    11881 cagcgccagt gatataaggc gtaacggcta acaatcttgg cgttaataca ttccgagcaa
    11941 ataaccgaag cgttactttt agtgacgggg ttatccaata cttttctagt cagtgtttag
    12001 tatttatgca agcgatataa gtagtcaccg cttagactat tgacattcat gatgcctgtt
    12061 aattcaaatt tcaggtatga acagtcattt attgttgtga tactgattcc gctagtttag
    12121 cataactgtc ctgcaaatca ttgttcaaaa aagaaagctc ctacaaactg tatcctgtat
    12181 ctctaccaga agataatata cttggttatt tttgtgcata agctcacata aaaataatat
    12241 taattatgtt ttcttagaga tagaaaaata aacaggtaag cagaagacgg acttttttaa
    12301 tgcgaaaacg tattcttttt attggcccac cgctgtacgg tttgttatac ccattgattt
    12361 ctctggctca ggcctttcgt gtaattggac atgatgtagt aattagtagc gctggcaaat
    12421 tcgcgaataa agcagcagaa gctggactgg ttgtttttga tgcagttcca ggtttagatt
    12481 cagaggctgg atatcgccat caggaagagt tgaggaaaaa aagtaatatt attggtcatt
    12541 tctctttttt tagcgatgaa atggcagata acctcatcca ttttgcagga aaatggaggc
    12601 cagatttaat agtctatccc cctcttggtc cggcaggccc attggttgct gctaaatata
    12661 gaattccttc agtgatgctg gctgttggat tcgcgcatac atctgcccat attcagatgt
    12721 taaaccgttc tttaagcaat gcttacaggc ggcatggagt cagcggtcca ctatgtgatt
    12781 tagcatggat tgatgttgct cccccaagta tgagcattct taaaaatgct ggagaaccgg
    12841 ttatctcaat gagatatatt ccttataacg gaggtgctgt aaaggaaaca tggtgggaca
    12901 gggattctga tcggaaacgt ttacttatca gccttggcac tgtaaaacca atggttgatg
    12961 gtctggagct gatttcatgg gttatggatt ctgcaaatga agttgatgct gatatcattt
    13021 tgcaacttgc aataaatgct cgtactggtt tacgaaaact accatcaaat gtacgtctgg
    13081 ttgactggat acctatgggt gtattcctta atggagctga tggatttatt catcacggtg
    13141 gcgcaggtaa taccctgaca gcgctgtata gtggaatacc acagattgtg tttggcgaag
    13201 gtgcagatcg ctctgttaat gcagaaattg ttgcgaagcg tgggtgtggg attattccgg
    13261 acaagcatgg actgactagt gatttggtaa atcgcctgct ttatgatgat tcactacgct
    13321 tctgttcaga tcaggtagcc gctgaaatgg ctgaacaacc cagtcctgca gagattgcag
    13381 aggttttgat gagaaaatta aaaaacaacg ggaaacaatt gtagcgtatt gctgaaattt
    13441 tcaacgagaa atgttatgag taatataatg tccttcatca cataaatgaa ggacatcact
    13501 aaataaatga gatagtctcc cataataata ttgcagaaag agttatcgga cgataaaaag
    13561 atattttaca cagagttatt atttgatacc ttcctgcaat tataatttgc taattcatca
    13621 ataattgctc cgcgccacca agcataatcg tgaccaccgg tatagacagt gaggttactt
    13681 tccaaaccag ctgcgattaa cgaatgatgc aatcgctgaa catgtgagac ggttgtacct
    13741 tctaaagaac cgactccaag tcgaatattt acatctttcg gaggcgctga aagtatctgc
    13801 tcacttaccc aggaggtatt attctcagta aacaagatcg ggctgccctg gtcagggttc
    13861 caccacattg aaggtgagtg actaatgatt gtaccaaatg ttgtcgacgc atatatagct
    13921 gccatcagtg ctgtcactcc accgaggctc tgcccggcca gtacagtatt agagcgacca
    13981 gcccatacga tatccggata gtctctgtat aactggggta tcagattttc cgctatatca
    14041 aagatgagtt ctttattgcc tcccagtata ttcatacgat cagattcatt aatattgtct
    14101 atccccataa ttgccattgg cgtgatacga ccagtggtga tggccatatc taatgcccgg
    14161 gtaatatcta tccggtcaaa ccatgtttca gcatcaggta acacaagtaa tccaagaggt
    14221 actgatgttg gattcagggg aaaatataaa cgaatccggc gttgatatcc tgcaacaaag
    14281 gaatgtaatg ttgaaagaat acctgtacat ttatgggaag tatcatccca ttccttttgt
    14341 tcaggagcca tatcaagaga aaggactgat tctccgaatc ctcgtatatt tatttctgct
    14401 gttttgttaa atggatcaga tttaccgggt aatggagaga agcgacttcc tagttgaaat
    14461 atgtcttttt gtgtcatatc tgtgggaatt tctataaatg agtatgagcc ccgatatgaa
    14521 gctggtaaca ccagtgtcag catccagata tctgtcgaag ggatatgagt cattagtcct
    14581 tttttgacgt cttttttatc tgttacacga ttcaggcgaa gatatacgcc ttgcagtgct
    14641 ttctctgagc gccacagaaa tgtaacctcc cgattttctg tagtggtgct ttttgtgatc
    14701 actggaagtg ttgctgactc aagatagtgc cagaaattag caatatctgt tgttcttatt
    14761 gtgacaggat gtgtcatttt ttttaactgt acacaaatat cttcgggtgt ctggaaaaga
    14821 tagtttttca taggtatcat cttaaattaa tccaagtgta agaataatac atcaaatgac
    14881 gttcattcaa gggatgatga gtgactttcc ggaattaaat ctgttgtatt gatgataaat
    14941 ataaaacagg tttaataaaa tttttgcttt atactgtaga tttcacgtat gtaagtaata
    15001 tatgaaaagg ctctcgttaa gatgtctggt acaaaatgac agatcatatg cctttgtaaa
    15061 gagacgacag aagtagagtc ttacaggcac atcataaata ccctccggcc atgccggagg
    15121 gtatttattt aactgccgct gtttgcactg gatgtggaac tactcatact ataagaaggt
    15181 ccacttcccc ccggatacat gatgatggat ggagtacccg gagcccagca tgctccatta
    15241 gcatcaatat atgttcccat atcttttcct cgtagatcta ttacattagt agaactaggt
    15301 aatccattaa gattcatccc tcctttcacg aaatcaagca tgtgatctga tatttctctc
    15361 attttaaaga cctcctgctc atttatgttc agatattaat ttggtgttgc aaccatttat
    15421 tatcacagaa ttcagaatat cattcagttt tatttttgtt aaaatatccc ctcttaagac
    15481 agacacatat tcttgtttat cattttcatt aaacaatgac tctatatttt ctacgtttaa
    15541 ataatatagc ccacaagcag cataaatttt agactcctta gttgactttc tattttttat
    15601 tatattttta aatatctctt cgggattatc taatgtaaga atgtgtgtgt ataatttttc
    15661 cccttcgctc tgatagccta taaacccatt catacctata gaaaaatagg ttgttttttc
    15721 taacgattgt tgaaggggag gtggatctga tgaaaatgca gcagatggta atataaacat
    15781 catacttatt ataatctttt tcgtaagata catactttcc cctttggttt tccatcgata
    15841 aatgttcaga taaacaatat atgttttttc ttgttaatgt ttatatcgat ggttttataa
    15901 catgaaatat tcagcataat aatcaacagg cttgtacctg atcaggtatc agtttgcaaa
    15961 aatgtttgtt ataggtcatg accgcaacca ggacacataa tttcaccaca accacaaata
    16021 taatcggcat catagtcatg tgttactata ccggaagccg acttattatt tgtgatacag
    16081 tgtgtctcct gctctacgga ggattctacc tctataggaa aattatcgtg ttttcgtctt
    16141 gaattgtctt gcttgttgcc tttatcaaag gtaacaatga attcttcatt tctgtgatta
    16201 ttatcacaat tcattcttat gcctcctcac atttcccgat gtatgaccgt cagtgctatt
    16261 agtgtgcgtt caagtctcag gttatacatg agcgacaacc atgagatcct gactaacaat
    16321 tgcatcagtg acataacgta aaatacctct tgactttata tggacaatat gacacttttt
    16381 tttgaaaagt aaataacaat cattctcttt tgtctttgtc tgggtgaggt caggtaagat
    16441 ggaatgtgct acaaacaggg tttctgtaaa tgacagtcgg tcagcgctgt catcaacttt
    16501 gctgttatct ttgatcatga gcgccactct actggaatat tctttatcga tgacctgact
    16561 ctcctgactg aggtgctaag atttggtaac ttttcataat gaaaataaag caacaggtag
    16621 ggattaatga gttataaaaa actgtcccaa ttgacggcta tattcagttt acctattact
    16681 atcttattgg tttcactttc atcccttcgg attgttggcg aagggaattc ttatgttgac
    16741 gtttttctaa gctttataat atttcttggt tttattgagc tgattcatgg gattcgaagg
    16801 attctggtct ggtcaggctg gaaaaacgga agttaaatat gatggagttt atatgcagtt
    16861 tatatgcgag aaataacaga atcacagtta agatatattt ccggggcggg aggtgcgcca
    16921 gctacttcag ctaatgctgc aggtgctgca gctattgttg gagctctcgc cggaatacct
    16981 ggtggtccac ttggggttgt agttggagcc gtatctgccg gtttgacaac agcaattggc
    17041 tcgaccgtgg gaagtggtag tgccagttct tctgctggtg gcggtagcta attata
//
LOCUS       DAZGCQ010000101        17011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000101 DAZGCQ010000000
VERSION     DAZGCQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.101"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1320
                     /gene="paoC"
                     /locus_tag="ACHK07_003846"
     CDS             <1..1320
                     /gene="paoC"
                     /locus_tag="ACHK07_003846"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO8104124.1"
                     /translation="DQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHTGLR
                     LATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADPTRR
                     FSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGARVHL
                     EQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGGQWG
                     ANTSTSGVYAACMKLREMIASTVGFDPEQSQFADGKITNGTRSATLHEATAGGRLTAE
                     ESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARSQVI
                     GAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPISSPM
                     KAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1330..2286
                     /gene="paoD"
                     /locus_tag="ACHK07_003847"
     CDS             1330..2286
                     /gene="paoD"
                     /locus_tag="ACHK07_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO8104125.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCEREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLADVASVRLHQEEDSYLPPSS"
     gene            complement(2464..3591)
                     /locus_tag="ACHK07_003848"
     CDS             complement(2464..3591)
                     /locus_tag="ACHK07_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104126.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3733..4791)
                     /locus_tag="ACHK07_003849"
     CDS             complement(3733..4791)
                     /locus_tag="ACHK07_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8104127.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLRYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5056..5940
                     /locus_tag="ACHK07_003850"
     CDS             5056..5940
                     /locus_tag="ACHK07_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8104128.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            6183..6239
                     /locus_tag="ACHK07_003851"
     CDS             6183..6239
                     /locus_tag="ACHK07_003851"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104129.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(6643..6921)
                     /locus_tag="ACHK07_003852"
     CDS             complement(6643..6921)
                     /locus_tag="ACHK07_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104130.1"
                     /translation="MPMYIKLILSIVRRISVNTICSLIVVVTLSLLSFSSVAKTITAV
                     GSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(7087..7353)
                     /locus_tag="ACHK07_003853"
                     /pseudo
     CDS             complement(7087..7353)
                     /locus_tag="ACHK07_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            7359..7766
                     /locus_tag="ACHK07_003854"
     CDS             7359..7766
                     /locus_tag="ACHK07_003854"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8104131.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            complement(7893..7968)
                     /locus_tag="ACHK07_003855"
     tRNA            complement(7893..7968)
                     /locus_tag="ACHK07_003855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7933..7935),aa:Thr,seq:cgt)
     gene            complement(8083..9336)
                     /gene="proA"
                     /locus_tag="ACHK07_003856"
     CDS             complement(8083..9336)
                     /gene="proA"
                     /locus_tag="ACHK07_003856"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO8104132.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            complement(9348..10451)
                     /gene="proB"
                     /locus_tag="ACHK07_003857"
     CDS             complement(9348..10451)
                     /gene="proB"
                     /locus_tag="ACHK07_003857"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO8104133.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            10739..11794
                     /gene="phoE"
                     /locus_tag="ACHK07_003858"
     CDS             10739..11794
                     /gene="phoE"
                     /locus_tag="ACHK07_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8104134.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(11833..12234)
                     /gene="crl"
                     /locus_tag="ACHK07_003859"
     CDS             complement(11833..12234)
                     /gene="crl"
                     /locus_tag="ACHK07_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8104135.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(12292..13536)
                     /gene="frsA"
                     /locus_tag="ACHK07_003860"
     CDS             complement(12292..13536)
                     /gene="frsA"
                     /locus_tag="ACHK07_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8104136.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(13628..14086)
                     /gene="gpt"
                     /locus_tag="ACHK07_003861"
     CDS             complement(13628..14086)
                     /gene="gpt"
                     /locus_tag="ACHK07_003861"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8104137.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            14347..15804
                     /gene="pepD"
                     /locus_tag="ACHK07_003862"
     CDS             14347..15804
                     /gene="pepD"
                     /locus_tag="ACHK07_003862"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8104138.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(15861..16169)
                     /locus_tag="ACHK07_003863"
                     /pseudo
     CDS             complement(15861..16169)
                     /locus_tag="ACHK07_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(16189..16397)
                     /locus_tag="ACHK07_003864"
                     /pseudo
     CDS             complement(16189..16397)
                     /locus_tag="ACHK07_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(16588..>17011)
                     /gene="yafP"
                     /locus_tag="ACHK07_003865"
     CDS             complement(16588..>17011)
                     /gene="yafP"
                     /locus_tag="ACHK07_003865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8104139.1"
                     /translation="PGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDESRWKEKLA
                     KSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIKSESELTVDAS
                     ITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
BASE COUNT         4211 a         4430 c         4331 g         4039 t
ORIGIN      
        1 gaccagagcg ggaaaatcac cgctatctca catgaaagct ggtccggaaa cctgcccggc
       61 ggcacgccgg aaacggcggt acagcaaagc gaattactct acgccggggc aaaccgtcat
      121 accggcctgc ggctcgccac gcttgatttg ccggaaggga acgccatgcg tgcgcccggc
      181 gaagcccccg gtctgatggc gctcgaaatc gcgatcgacg aactggcgga aaaagcgggc
      241 atcgatcccg tcgagtttcg catcctgaat gacactcagg ttgaccccgc cgacccgacg
      301 cgccgcttct ctcgccgtca gcttatcgag tgcttgcgca ccggagcgga taaatttggc
      361 tggaagcagc gcaacgccac acccggacag gtgcgcgacg gggagtggct ggtcggccac
      421 ggtgttgcgg cgggctttcg caataatctg ctggaaaaat cgggtgctcg ggttcacctc
      481 gaacaaaacg gcaccgttac cgtagaaacg gacatgaccg acattggcac cggcagctac
      541 accattctgg cccagacggc agcggaaatg cttggcgtac cgctggagca ggttgcggtt
      601 cacctcggcg attccagttt cccggtttct gcgggttctg gtggacaatg gggcgcgaat
      661 acctccacct ccggcgttta cgccgcctgt atgaagcttc gcgaaatgat tgcctcgaca
      721 gtcgggtttg atcctgagca gtcgcagttt gccgacggca agattaccaa cggtacccga
      781 agcgccacgc tacatgaggc caccgcaggc ggcagactga cagcggaaga gagcattgaa
      841 ttcggaacac tgagcaagga gtaccagcag tcgacctttg ccgggcattt tgtggaggtc
      901 ggcgtgcata gcgcgacggg agaagttcgg gtccggcgta tgctcgctgt gtgtgctgca
      961 ggacgcatcc tgaatccgaa aactgcacgc agccaggtca ttggcgcaat gactatgggc
     1021 atgggcgcgg cactgatgga ggagctggcg gtggatgacc gtttgggcta cttcgttaat
     1081 cacgatatgg cggggtatga ggtgccggtc catgcggata tcccaaaaca ggaggtgatt
     1141 ttcctggatg ataccgaccc catatcctcc ccgatgaagg ccaaaggtgt cggtgagctg
     1201 ggcctgtgcg gcgtgagcgc ggctatcgcc aacgcggtgt ataacgccac cggtattcgg
     1261 gtacgggatt atcccatcac tctggataag ctgctcgata agctgccgga tgtggtttaa
     1321 ggaggaacaa tgtcataccc gctttttgac aaagacgaac actggcataa gccagagcag
     1381 gcgtttctca ccgatgacca ccggaccatt ctgcgcttcg ccgtagaggc gctaatgtcc
     1441 ggtaaaggag cggtgctggt gacgctggtg gagatacgcg gcggcgcggc gcgcccgctc
     1501 ggggcgcaga tggtggtgcg cgaagatggt cgttactgcg gttttgtctc tggcggctgc
     1561 gtggaggccg ctgccgcttt tgaagcgctg gagatgatgg gctcaggctg cgaacgcgaa
     1621 attcgctatg gcgaaggttc gccgtggttt gacatcgttc tgccctgcgg cggtgggatc
     1681 acgctgacgc tccataaact acgctcggca cagcctctgc tcgccgtgct gaaccgcctg
     1741 gaacagagaa aaccggcggg gctgcgctac gatccgcaag cacaatcgtt ggtgtgcctg
     1801 cccacgcaaa cccgaacggg ctggaatctc aatggctttg aggtggggtt caggccatgc
     1861 gtcaggctga tgatttacgg acgttctctt gaggcgcagg caaccgcgag tcttgcagca
     1921 gccacaggct atgacagcca tatcttcgat ctttttccgg cctcagccag cgctcagatc
     1981 gataccgata cggcggtcat tttgctgtgc catgatctca accgagagct gccagtgttg
     2041 caggccgcgc gagaggcaaa acccttttat ctcggcgcat tgggcagcta tcgaacccac
     2101 acgttacgtc tgcaaaagct ccacgagctg ggatggtcca gggaggagac agcgcaaatc
     2161 cgggcacccg tcgggatatt tcccaaagcc cgggatgcgc atactctggc actctccgtg
     2221 ctggcagacg tcgcctctgt acgtctccat caggaggagg attcatacct gcccccgtcg
     2281 tcctgatcct tgcggccggg cgtgggaagc gctttctcac ctccggggaa ataccataaa
     2341 ttaagagaaa aatctgcagt agccgtttta gaaaatcacc taaatcgttt aaaagagcgc
     2401 cctacccata gtgggtgctc atcgccttgt gtaacttatt cagcaacgcg cgccctcgcc
     2461 ggactaaagc gcaccggcct tctggcgtgc ggtaacaaag gtcagtacgc ccgcgcaaag
     2521 gagcagcaca ctactcatgg caaacgtact ctgccagcca aggcggtcaa acacgatgcc
     2581 tcctgccgtt gatccaaggg cgattgagag ctgaatcacc gcgaccatga gaccgcctcc
     2641 ggcttcggcg ttgtcaggca acgtacgcgc aatccatgtc caccatccgg ttggcgcggc
     2701 ggtggccagc atgccccaga gtcctaacag cagcgagacc gtccagacgt gatgcccggt
     2761 aagcatcaga ccgatggcaa tgacggccat cagtccgggt atggccatca gcgtggggta
     2821 gaattttctg ttgagaaatg tcgaaacaac cagcgtgcca ataaaaccgg ccacgccgat
     2881 aatcagcaaa atcaacgaca agcctaagga attaacccgt gtcaccgact ccagaaacgg
     2941 gcgcacatag gtgaatagcg caaactgccc catgaaaaac aggccacaag ctatcatgcc
     3001 gaccacaacc atgcggcggc taaataagcg gaataccgcg ccacatgacg tgtggctttt
     3061 attcgcatcc atgctgggca gactaatgca ctgccagata aaggcgacaa ccgccatggg
     3121 caccaggcac aagaaagcgc ctcgccatcc gaccgtagcc cccagatagc ttcccagcgg
     3181 cgcggccacg accgtcgcca gcgcattacc ggcgttgaaa atcgccagcg cacgcgtgac
     3241 ctgatgctgc ggcaccagac gaatcgccgt tgccgctgac atagaccaga atccgccaat
     3301 ggccacacca atcatcgcac gaccgaccat atacatcaga taactggtag ccagcgcaat
     3361 aataagcccc gatacggcca tcagaaccgt catccccagc agcaggaact tacgattcat
     3421 ctttccagct agcgttgaaa gcgtcaggct ggtcagaaca gccagcgcgc cggagatagc
     3481 aatcccctgc cctgcaagcc cctccgtcac gcctaaatcg cgggtaatcg gcgtgagcag
     3541 gctgacgggc ataaattcag aagcaatcag cacaaacacg cacagggtca tagcaaaaat
     3601 accgccccag tatgcgtgct gatgatgagg tgatggtgtt tggcttagtg tcgacatagt
     3661 gaaatttcac aaagatcagg ataaaagcca cctgccatag accggtggct ttgcatcaat
     3721 tcagaagagg ttttatttca gtttggtctg gaaaaattgt tcgaacttcg cgaaaggtat
     3781 ttttccggcc acgttatcgt acaggtccac gtgatttgcc cctggtacca ccaccaactc
     3841 tttgtcttta ctgccaaccg ccttgtaagc atcttcagca aaatagcgtg aatgcgcttt
     3901 ctcaccggtc acaatcagcg tcgggatggt gatttcactg gcgtatctca acagcggcat
     3961 attcataaat gacagcggca tcgttgccgt ccacgccccg gtagagttaa cggaacgttc
     4021 gtggaagccg cgcggcatgc gatagtagtc aaagaactct ttcagtaccg gatgaggatt
     4081 tgccggcagc gtttctggca gaatgcgctc gcccgcactg accttgccgt tgctgtcgac
     4141 attaatatca tgaccaccgt gagcaaacgt tccgctctgc gcatccttcc agcgctgttc
     4201 attcagatat tgcagaacag cacgacggtc ggcggtagaa taacggtctt tgccatcccc
     4261 cacaccatga cccatcgccc ggctcatgtc atacatcacg ctggtagcta ccgctttaac
     4321 gcgggtatcc atcgccacgt cgttcagcgc catgccgccc cagccacaaa taccgagcag
     4381 cccgatacgg ttgcggtcta cctctttttg cagccctaag aaatccaccg ccgcgctgaa
     4441 atcttcagtg ttgatatccg gtgaggcgac gtttcgcgga tagccgccgc tttccccggt
     4501 gtaagaagga tcgaacgcca gggtaacaaa ccctcgttcc gccagcgtct gcgcatacag
     4561 gccgctggat tgctctttca ccgcgccaaa tggcccactg accgcaatcg ccgccagctt
     4621 gcgatcgcca cggtctttag gcaggtaaag atcgcccact aaggtgatgc catatcggtt
     4681 cgggaacgag actttacggt gatcgacttt ctggctctcg gcgaatgttt tatcccattt
     4741 atctgtcatg gacacagggg cattaggatt ggttgaatca gcataactca ttgtcgtgac
     4801 tccacttaat gatgcgaaaa gcagcatggt cggcatcgcg gtttttagcg ttcgggtgaa
     4861 ggctttcata aaaatcccca taaaaaacaa cagagagata caggtttccg acggacaacg
     4921 ctgcggtgct ttactttgat ggggtcatta tagtgagcag aattagtgct gattaggggg
     4981 cgattaatgc taagattaat accatattgt tataaattaa tgcggagatt gccggcatga
     5041 aggggggatg tttcaatggc gaaaagggag aactacaacg agctgtacct gtttatgcag
     5101 gtggtgcggg aaggcagctt taccgcagcg gctcagcggc tgggtcttgc tcagtcagga
     5161 gtcagccgtt ctgttcgcga gcttgaagaa aggctgggtg tccagctgct agtgcgcacc
     5221 acgcgtaaac tgtcgctgac acaagcaggc gagcagctct accagaagac ggcatctgga
     5281 tttgaaatgt tagatcttgg gcttgccaca ctggcgcact atcgagagac gccctccggc
     5341 acggtgcgta tcaatgccag ccagcacgcg attgataaat gcctgctgcc aaagctggcg
     5401 gtatttaaac agcgatatcc tgatatccgg cttgaactga taaatgagag ccggttcgtc
     5461 gatatcatcg aggaaagatt cgatgcaggt gtccgcctgg ggcctgaagt cggccagggt
     5521 atggtcgcgg tacgcatcac gcccgatatg gagatggcaa ttgtggggac tccggaacat
     5581 tttcgtcgct atggttttcc gcaaactccg gcggatttaa aggcgcatcc ctgtatcgcc
     5641 tatcagtttg ccgacggcag cgtataccag tgggaactca atcaggatga taaaaaaatc
     5701 actcatcgcc ctgaaggaca atgggcccta tccgacagct atatggaagc agaagccgcc
     5761 cggctgggcc tcggattggc ctatgttcct gttgaactgg tcgcggatga tttagaacac
     5821 ggtaagctta tcagggtgtt gcagcgttac agcctgcgaa tggagggatt gtttctctat
     5881 taccctcatc gcaacgtctc ccccgccctg cggatggtca ttgatacatt gaaaatctga
     5941 acgttaataa cgaccgactc ttcactgtgc aaaattgtgt caggtagtcc ctctgcgttc
     6001 aattccaggg tctgctttga gcgagaaaga tcagacttat ctgtacaagg ggactatcca
     6061 gcaatggaga tcatccaaaa gaaaaaacca cctggctccc ctcgacaacc ttaatccggg
     6121 catttagcct caatcaacat tttcatatct gtcaggttac ttcattatga aaaccgatca
     6181 atatcgttgt ttttgttgca acagtagaat acgcgtattt atccctccat ttctattaat
     6241 aacggcaata aactgttcat ttcactgata tttaaaatat gcatcctttt caagctgttc
     6301 agcggcataa gaaaacgtat taaatatgaa ttcgacgatt taaccatttc tgtctgattg
     6361 gtaatcatct gtgattaatg ctggtctatt gattacgcca gcattaaccg cttagggatg
     6421 tattcttcct gttgatatac agccctcgaa aaagagagaa aataaaactg tgtccaatat
     6481 tactctacca ctccagaccg aagcatcggt agaattagca gaacgactgg tacaatagat
     6541 tgggatagcg tcatttgtca ggtattccat tgcctgacaa atgacaattt tgagtcaata
     6601 gacgtgcttc ttgatcatgt cttaaaaatt ataccatgag ccttatgaag catcattttt
     6661 actgactggt gttaattttg ctatcatata aactctgttc ttaaaaaacg cgcccagaat
     6721 tttatatgat ttcccttgtt tttctgcctg taaagaaatt tctttttcag tgctgtcaat
     6781 ggttgaaccg acggcagtaa tcgttttggc gacgctactg aatgataata aagacagtgt
     6841 aacaacaaca attaaagaac agatagtatt cacgctaatt ctcctgacaa ttgagaggat
     6901 gagttttata tacataggta ttaaacatga gtcatattta ccccaagaga aaatactcct
     6961 tctgtaatta aaccttcatc tccttcgggg tgagcaatac ataatgcatc ttgctatgaa
     7021 aaacacaggg atatataaat cttccttctg acctatttaa taaaacatga atgtaaaagt
     7081 tattcttcag tttgcagatg cagtgggtct gatggtaatt tcggttgtat caacgctttc
     7141 gggagattca ataatatgcc ttaccgcacg ggcaatatcc gcaggttgaa gtgcaacaga
     7201 acggtaggcg tccattgcct tcatggtttc cacatgggta atggttgaag ccagttcact
     7261 ttcgacaacg ccaggattta cgcaggtcac acggattttc gagctttcct gccgtaagcc
     7321 atcagaaata gcccgtactg caaatcaggc agcgtgaaat ggtcgttgcc tctccctcct
     7381 atctcgcagc caacagcaca cctgttcatc cgcgtgaact tgtgaatcat aagtgtatcg
     7441 gctggcgtca atccccggat gttgcgcctt atcgctggcc atttgaagaa aacaggagag
     7501 ctttcgacct tgcgattgaa ccgcagatta cgactaatga tctgcgtctg atgttgaggc
     7561 tggctcttgc cggcggagga ataacaattg ccactcagga aactttcagg ccatatattg
     7621 aaagcggtag gcttgtatct ctgcttgatg actttcttcc acaatttccg ggcttctatc
     7681 tgtatttccc acagcgtcgc aatattgcac caaagctccg cgccctgatt gaccacgtca
     7741 aggaatggcg gcagcaattg gcttaaatgt ctgcacctgc attgcctgat gtcagaacag
     7801 tattttgatt aattgccagg gttacaatgg cataaatacg gcacaggagg aaacgtggtg
     7861 tatttgaata tggggtaact acttgattta aatggtgccg ataataggag tcgaacctac
     7921 gaccttcgca ttacgaatgc gctgctctac caactgagct atatcggccc tgaaaggaca
     7981 tgttcacgaa cgtgaatcac ggtggacaag gttaaaacta accgggcgat gcgtcaatgg
     8041 ccttgtgaat caaatggcta cttttgcatc acccggtttt atttacacac gaatggtgta
     8101 atcaccaatg ccgatccact tgtaagtggt cagtgcttcc agccccattg ggccacgcgc
     8161 gtggagtttt tgtgtgctta ccgccacttc cgcacccagt ccaaactggc cgccgtcggt
     8221 aaaacgcgta gaggcgttaa cgtaaacagc ggacgaatcc acttcgttaa caaaacgctg
     8281 ggcgttgcgc atatcgcggg tcaggatcgc atcggagtgt tgtgtgccgt gttcacgaat
     8341 atgggcgatg gcatcgtcaa gatcgctgac gattttgacg ttcaaatcta atgacagaaa
     8401 ctcatcgtca tactcttcgg ctttaaccgc caccaccttc gcggggcctg cctgcaactg
     8461 tgtcagcgca gctgcatctg cgtgtaatgt cacgccgctt tccgccattt gtttgcttaa
     8521 tgcgggcagg aagctatcgg cgatgttttt attcaccagc aacgtttcta ccgtattaca
     8581 tgtgctcgga cgctgagttt tcgcgttgac gatcactttt aatgcttcag cgatctctgc
     8641 actttcatca acgtaaatat ggcatacgcc tataccacct gtgatcaccg ggatcgtcga
     8701 ctgctcgcgg cacagtttat gcaaaccagc cccgccgcgc gggatcagca tgtcgatgta
     8761 tttatccata cgcagcattt cactgaccag cgcacggtca ggattatcaa tcgcctgcac
     8821 ggcaccagcc ggtaagctac aggatttcag ggcgtcctga atcaccgcca ccgttgcagc
     8881 gttagtgcga caggtttctt tgccaccgcg caggatcacc gcgttacctg ttttcaggca
     8941 cagcgaagcg acatcaaccg tcacgttcgg gcgcgcttca taaatcacgc caataacccc
     9001 cagcggtacg cgacgacgct caagacgcag gccgctgtcc agtacgccgc catcgattac
     9061 ctgccccacc ggatcggcga ggttgcacac ctggcgcaca tcatcggcaa tgcctttcag
     9121 ccgtgcgggc gtcagtgcca gacggtcaag catcgcttcg ctgaggccat tggcacgcgc
     9181 gtcagcaaca tcctgggcgt tagcgttgag gatgatttcg ctttgtgctt ccagttcatc
     9241 ggcgatttta tccagcacgc gatttttttc gcggctggag agttgcgcta atttatacga
     9301 ggcttgcttc gcggcaatgc ccatttgttc cagcatcagc ctgctcctta acgggtaatc
     9361 atgtcatcac ggtgaacggc aaccgggccg tattcatatc ccagtattgc atcaatttct
     9421 tgcgagtggt gtccggcaat acggcgtaat gcatcgctgt tgtaacgact gacgccgtgg
     9481 gcgatatcgc gaccttcgag gttacaaatg cggatgactt caccacgcga gaagttgcca
     9541 gtcacgcttt taatgccttt cggcaacagg gagctgccgc gttcaagaat ggcggcggtt
     9601 gccccttcat ctaccgtgat ttcacccgca ggcggcgcac cgaaaatcca gcgtttacgg
     9661 ttttcaagcg gagtcgcctg ggcatggaac agcgtaccga cggaaatgcc ttccatcaca
     9721 tcaccaatga cgcccggctt gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa
     9781 gccacgtccg cggcctgcaa tttggtactc atgccgccag ttccgaggcc tgaaacgctg
     9841 tcaccggcaa tcgcgcgcag tgcgtcatca ataccgtaaa catcttttat caattctgcc
     9901 tgcggattac tgcgcgggtc agcggtatac aaaccttttt gatcggtcag taacaacagt
     9961 ttatcggcac ccgccagaat cgccgccagt gcagaaaggt tgtcgttatc gccgacttta
    10021 atctctgccg tagcgacagc atcgttctca ttgattaccg gaacgatatt gttatcgagc
    10081 aacgcacgca gggtgtcgcg ggcgttcagg aagcgttcac ggtcttccat atcagcacga
    10141 gtcagcagca tttgcccgac gtgaatgcca taaatcgaaa acagctgttc ccacagttga
    10201 atcagtcgac tctgccctac cgccgccagc agttgtttcg aggcgatagt cgctggcagt
    10261 tccgggtaac ccaggtgctc acgtccggcg gcgatcgcgc ccgacgtcac aataacaatc
    10321 cgatgcccgg cggcatgtaa ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg
    10381 ttcagacggc gcgatccgcc tgttagcaca ctggtgccga gttttaccac cagcgtctgg
    10441 ctgtcactca tgattctctg ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt
    10501 agcaggactg tcgtcggttg ccaaccatct gcgagcaaag catggtgttt tgttgcgcgg
    10561 gatcagcaag cgtagcggca gttgtttacg cttttattac agatttacta aattaccaca
    10621 ttttaagaat atcattaatc tgtaatatat ctttaacaat ctcaggttaa aaactttcct
    10681 gttttcaacg gggctctccc gcagaatatt cgcgcgttaa ttaaaatcag gaatgaaaat
    10741 gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat ccgtacaggc
    10801 cgcagaaata tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat
    10861 gcattatatg agtgataacg acagtaaaga tggcgaccag agttatatcc gttttggttt
    10921 taaaggcgaa acacaaatta acgatcaact gactggttat ggtcgttggg aagcagagtt
    10981 tgccggtaat aaagcagaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg
    11041 attgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
    11101 cgtggaagcc tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga
    11161 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
    11221 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
    11281 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
    11341 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
    11401 ccgtggcaca ggcaagcgtg cagaagcgtg ggcaacaggt ctgaaatacg atgccaataa
    11461 tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa ctggcggctt
    11521 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgatt ttggtctgcg
    11581 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
    11641 tctggtcaat tatatcgatg tcggggctac atattatttc aacaaaaata tgtcagcgtt
    11701 tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga
    11761 tattgtcgcg gttggcatga cctatcagtt ttaatgatta ttgccggatg tgatgcatcc
    11821 ggcacatttc actcacgccg ttaacttcac cggctcgtca cgaaaatcat ccgccggttc
    11881 cagcttcaga ttcagcgtcg tcagcagttc acgcagtttc tcgtgaaact cacgcagggt
    11941 gtgctccagt cgttcaacca cttcagtgtc ttttaccgga acgctcttcc agtcgcctgc
    12001 tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc
    12061 catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc
    12121 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
    12181 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat
    12241 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa
    12301 gcgtttttcg atccagtcgg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
    12361 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
    12421 ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
    12481 acgtcccagc aatccttgca cttttaatga ataacgattc agctccacgc gcaacgcttc
    12541 atcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac
    12601 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
    12661 cgctttcaga cgcggcgatt caagatatgc cagacgcacg gcaacgttag cgccgaaacg
    12721 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa
    12781 gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgatg aaaagcccac
    12841 cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
    12901 gctgtaatag tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg
    12961 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
    13021 ctccatctgc cgcatcgtgc ccggcagacg ctgagcggcc tcttcatagg cgcggtttga
    13081 caaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt
    13141 gtacaacgta gccgcatgca gccagtgacg accactgagt tgcggatctt cttccgcaca
    13201 ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt tgccgccacg
    13261 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
    13321 acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg
    13381 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
    13441 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
    13501 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
    13561 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
    13621 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
    13681 cgatccaggt atcttgcggg atatcaacaa cataatcatc aaccagcgga cgaccagccg
    13741 gttttgcaaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
    13801 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
    13861 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
    13921 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc
    13981 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
    14041 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
    14101 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
    14161 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
    14221 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga
    14281 caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga
    14341 cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa
    14401 atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt
    14461 tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cttgattcgt
    14521 aaacctgcta cagcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat
    14581 atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct
    14641 tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt
    14701 ggtatggcct ctgcgctggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa
    14761 gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt acagagcaac
    14821 tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg
    14881 ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt tagatcgtga agaggttcca
    14941 gctggttttg aaaccttcaa gttaacctta aaaggtctga aaggcggtca ctccggcggt
    15001 gaaatccacg ttggcctcgg taatgccaac aaactgctgg tgcgcttcct ggcgggtcat
    15061 gcagaagaac tggatctgcg tcttattgat ttcaacggcg gcacactgcg taacgccatc
    15121 ccgcgtgaag cctttgcgac cattgctgtc gcggctgata aagtcgacgc cctgaaatct
    15181 ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg
    15241 gccttgttgc tggactctgt tgcgaacgat aaagctgccc tgattgcgaa atctcgcgat
    15301 acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaattc cgacgtggca
    15361 aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga caataatgta
    15421 gaaattcact gtctgatccg ttctctgatc gacagcggta aagactacgt ggtgagcatg
    15481 ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag cgaaaggtgc atatcctggc
    15541 tggcagccgg acgcaaactc tccggtgatg catctggtac gtgaaaccta tcagcgtctg
    15601 ttcaacaaga cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa
    15661 aaaccgtatc cggaaatgga catggtttct atcgggccaa caatcaccgg tccacactct
    15721 ccggatgagc aagttcacat cgaaagcgta ggtcattact ggacactgct gactgaactg
    15781 ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct
    15841 tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa cgttcgtcgc
    15901 gggttgccac gttcaatctg gtgatggaac attcgccgct gtgatttcag cgccgcgcta
    15961 ttttcctgtt gctgctgctc cagcctccag gcaatcagca atcgcgccag ccgtttgtta
    16021 gcatgctggc tgcgctctga ctgaaccttc acgctaatac cggatgccag atgcgtggcg
    16081 cgtaccgcca agtcggtttt attgacattt tgaccgcccg gccccgacga acgcagcgtg
    16141 tcaggataac gcccggcttc ggtttccagc accgttaccg cgacgtcttg ccgggtaaaa
    16201 acgaccaaca gagaagaacg ctgcgccaat cgggtagccg cgtccggggt gtgtaaatct
    16261 ggcatcccca ctacgcgctg catgttgggt aaatttgccg tggtgtgtaa ttgttggatc
    16321 gctaaactct cgatccacag gtcatcagat gcgatggtaa ataccgcatc ggataaggga
    16381 cgaatatatt tgcccatgta ccattccatt tatgaataaa aatcaggaaa tggcagacga
    16441 atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc attgataaag
    16501 gatttgtcat gatttacctc cttgcagttt gtggaatcag aaaacgcgca cataccaggc
    16561 gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatatcgca tataaaaatt
    16621 agtaaaccac gctccccggc attcaacgcg ctgctgctta actgtctgaa aaccataacg
    16681 ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta agttcggatt cagacttaat
    16741 ccaaggtttt aacaaagcac tggcaacccc acggcgggtg tattcagggt caacaaataa
    16801 catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat taatgactgc
    16861 aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa tctgcgccca
    16921 ggcagcaatt tgttgtggtg aataatgctg actggctgtc atcgtaaccg ctctaatgaa
    16981 aatagcgcat agttgctgaa aatcgccagg c
//
LOCUS       DAZGCQ010000102        16983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000102 DAZGCQ010000000
VERSION     DAZGCQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.102"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..511
                     /gene="trmL"
                     /locus_tag="ACHK07_003866"
     CDS             38..511
                     /gene="trmL"
                     /locus_tag="ACHK07_003866"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO8104140.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(564..1385)
                     /gene="cysE"
                     /locus_tag="ACHK07_003867"
     CDS             complement(564..1385)
                     /gene="cysE"
                     /locus_tag="ACHK07_003867"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO8104141.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1465..2484)
                     /gene="gpsA"
                     /locus_tag="ACHK07_003868"
     CDS             complement(1465..2484)
                     /gene="gpsA"
                     /locus_tag="ACHK07_003868"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO8104142.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2484..2951)
                     /gene="secB"
                     /locus_tag="ACHK07_003869"
     CDS             complement(2484..2951)
                     /gene="secB"
                     /locus_tag="ACHK07_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO8104143.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3014..3265)
                     /gene="grxC"
                     /locus_tag="ACHK07_003870"
     CDS             complement(3014..3265)
                     /gene="grxC"
                     /locus_tag="ACHK07_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO8104144.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3406..3837)
                     /locus_tag="ACHK07_003871"
     CDS             complement(3406..3837)
                     /locus_tag="ACHK07_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO8104145.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4082..5626
                     /gene="gpmM"
                     /locus_tag="ACHK07_003872"
                     /gene_synonym="pgmI"
     CDS             4082..5626
                     /gene="gpmM"
                     /locus_tag="ACHK07_003872"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO8104146.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKNRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5660..6919
                     /gene="envC"
                     /locus_tag="ACHK07_003873"
     CDS             5660..6919
                     /gene="envC"
                     /locus_tag="ACHK07_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO8104147.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6923..7882
                     /locus_tag="ACHK07_003874"
     CDS             6923..7882
                     /locus_tag="ACHK07_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO8104148.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPNAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(7869..8903)
                     /gene="waaH"
                     /locus_tag="ACHK07_003875"
     CDS             complement(7869..8903)
                     /gene="waaH"
                     /locus_tag="ACHK07_003875"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO8104149.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNHGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(9142..10167)
                     /gene="tdh"
                     /locus_tag="ACHK07_003876"
     CDS             complement(9142..10167)
                     /gene="tdh"
                     /locus_tag="ACHK07_003876"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO8104150.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(10177..11373)
                     /gene="kbl"
                     /locus_tag="ACHK07_003877"
     CDS             complement(10177..11373)
                     /gene="kbl"
                     /locus_tag="ACHK07_003877"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO8104151.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(11648..12505)
                     /gene="yibB"
                     /locus_tag="ACHK07_003878"
     CDS             complement(11648..12505)
                     /gene="yibB"
                     /locus_tag="ACHK07_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO8104152.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     VLENEMIIFTSPDLKPRVEAIRNGKPTTVIAIDIKKKFRHIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQYKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSGLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            12809..13741
                     /gene="rfaD"
                     /locus_tag="ACHK07_003879"
     CDS             12809..13741
                     /gene="rfaD"
                     /locus_tag="ACHK07_003879"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO8104153.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            13751..14797
                     /gene="rfaF"
                     /locus_tag="ACHK07_003880"
     CDS             13751..14797
                     /gene="rfaF"
                     /locus_tag="ACHK07_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO8104154.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            14801..15793
                     /gene="rfaC"
                     /locus_tag="ACHK07_003881"
     CDS             14801..15793
                     /gene="rfaC"
                     /locus_tag="ACHK07_003881"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO8104155.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            15790..>16983
                     /gene="rfaL"
                     /locus_tag="ACHK07_003882"
     CDS             15790..>16983
                     /gene="rfaL"
                     /locus_tag="ACHK07_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO8104156.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKRENIEKIVFFSFISALSLRCFSELIAYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSFIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVQNKYRSPCNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLA"
BASE COUNT         4396 a         4292 c         4170 g         4125 t
ORIGIN      
        1 attttctgct atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga
       61 accagaaatt ccgccaaata ctggcaacat catccgtctt tgcgctaata ccggctttcg
      121 tctgcatatc atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg
      181 gctggactat cacgagttta ccgccgttac gcgtcatcat gactacagcg cgttcctcga
      241 agcagaaaat ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc
      301 cgtaagctat caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc
      361 gagcattctt gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccagacag
      421 ccgcagcatg aacctgtcca atgcggtgtc ggtagtggtg tatgaagcct ggcggcagct
      481 gggatatccg ggagcggtgt tgagagatta gttacgccat gccggataga tgattacatc
      541 gcatccggca cgatcacagg acatcagatc ccatccccat actcaaatgt atggttaata
      601 ccgttgaaat gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaatg
      661 cgtgccggaa cgccagcggc ggtggtatgc ggcggcaccg gttgcagcac cacggaacct
      721 gcgccaatct tcgcgccgcg accaacttca atattgccga gaatcttcgc gcccgcgcca
      781 atcatcacgc cttcacgaat tttcgggtga cggtcgccgc tagttttccc agtaccgcca
      841 agggtgacgg attgcagaat cgacacgtcg ttttcaatca ccgccgtttc acccacaacg
      901 atacccgtgg cgtggtcaag catgataccg cgaccaattt ttgctgccgg gtgaatatca
      961 acctggaacg tcacagaaac ctggttttgc agaaagattg ccagtgcgcg acgcccctga
     1021 ttccacaacc agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac
     1081 aacggggttg agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag
     1141 gccgcagagg cgatcatttc cgggtcagcg gcgtaggctt cttccaccac ttcacggata
     1201 gcaatagcag gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcactg
     1261 ccaaggtttt cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag
     1321 tccgccagcg ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac
     1381 gacattgctt actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat
     1441 agcggtaaca aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc
     1501 taataacgtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc
     1561 ctcggttatt ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt
     1621 gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg
     1681 accgagcatc atgccaaaac ggcggttacg cgactggttg tcggtacagg taagcaccag
     1741 atcgcccagc cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag
     1801 acgcgacatt tcagccaggc cacgggtgat tagcgccgta cgcgcattcg cgccaaaacc
     1861 gataccgtcg gacatccccg cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg
     1921 cacgccaatg aaatccgggt tactgtaaac gcggaaactt ttaccgcagt gcagcagctg
     1981 ctgaagatca tcagcaaaag tttggtcggt cgaggccagc gaaatggctg tcggtaaacc
     2041 tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc
     2101 taacgcctcg cgggccacgt cctgtaacag acgcccggtt tccgcttcca gccctttggt
     2161 cgcccacacc agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc
     2221 aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag
     2281 atcgctttca agatggagtg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca
     2341 gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg
     2401 gccatttctt gccagggtaa tggcaagagc ggtgccgtac gagccggcac cgatcacagt
     2461 cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc
     2521 tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc
     2581 gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg
     2641 ttcgggcagt atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag
     2701 aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt
     2761 acggtaacac gcagtaccac ttcgtaaacg tcatctgcca gttgagtaga tgccgtatcc
     2821 agatcaagtt taacttctgg ctgccaatct ttctggaaaa cgtgcggcgc gttcggcgct
     2881 tcgaaagaaa tatccttggt ataaatacgt tggatctgga aagtcatttc ggtgttgttt
     2941 tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc
     3001 caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca
     3061 agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac
     3121 cgctgcgttt gatcatctct tcacgcttag cggcgttgcc atcgatcggc aactcctgaa
     3181 aactcacgcc cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt
     3241 tggtatagat ttcaacattg gccataactt agctcctgtt ttttaccctg aaatttcatg
     3301 ttgcagggcg gcagcaaaat ggagtgtccc cagaagctta taacaataag tcgctggggg
     3361 cctaattgca gctaacggcc tgcatcatga aagacgacag gtgaattatt tgccgcgcac
     3421 caaaggcaag ttttcgccag cccagccagc gacgccttct ttcagcacga atacttgcgc
     3481 aaaaccagct ttcgtcagtg cgtttgcagg ctcctggcac tgcatgccag aaccgtctac
     3541 cacgataacc ggtttgtctt tgtgcttctc aagctcacca acattgttgg ctttgatttc
     3601 gctcggcaac agattaatag aacctgcgat atggccttta cggaagtcat cacgctgacg
     3661 taaatccaca accacagcgt cttctttgtt gatcagacgc gtagcttcac cacgagtaat
     3721 caccttcact ttcgaggtca ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc
     3781 gatccaggcg atactcagta tgggatgacg gccaacaaat tgcataattt cttgcatggg
     3841 gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg
     3901 cggcaaatac agccagcgcg ttaactggaa tgcaattttg cggggcgcga cgaaaaaaaa
     3961 gcgtaaatct ggcatcggct tgccgcgagc ggtcgtatat tttgatcttc agaggctatt
     4021 ttatcgattc agctgtagta aaattacgca aattttgact cttgagtatg aggttgtcgc
     4081 aatgtcggtt tctaaaaaac ctatggtact ggtgattctg gatggctatg gctatcgcga
     4141 agaacagcag gataacgcca tttttagtgc taaaaccccg gtaatggatg cactgtgggc
     4201 caatcgtccg cataccctga tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca
     4261 gatgggtaac tccgaagtag gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga
     4321 cctgactcgt ctggacgttg aaatcaaaaa tcgcgctttc tttgctaatc cagtgctgac
     4381 tggcgcggta gataaagcga aaaacgcagg caaagcggta cacattatgg gtctgctctc
     4441 cgcaggcggc gtacacagcc acgaagatca catcatggca atggtagaac tggcagctga
     4501 acgcggcgca gaaaaaatct acctgcacgc attccttgac ggtcgcgaca ctccgccgcg
     4561 cagtgctgaa tcctcgctga aaaaattcga agagaaattt gccgcgctgg gtaaaggccg
     4621 cgtagcgtct atcattggtc gttactacgc gatggaccgc gataaccgtt gggatcgcgt
     4681 tgaaaaagct tatgacctgc tgactctggc gcagggtgag ttccaggccg ataccgccgt
     4741 tgctggtttg caggctgctt atgctcgtga cgaaaacgac gagttcgtga aagcgaccgt
     4801 tattcgtgca gaaggtcagc cagatgcggc aatggaagac ggcgatgcgc tgattttcat
     4861 gaacttccgt gctgaccgtg cgcgtgaaat cactcgtgct ttcgtgaacg ctgatttcga
     4921 tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg atgctgaccg aatacgccgc
     4981 tgacatcaaa actgcggttg cttacccacc cgcttccctg gttaacacct tcggcgagtg
     5041 gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa accgaaaaat atgcccacgt
     5101 tactttcttc tttaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat
     5161 caactcaccg aaagtggcta cctacgatct gcaaccggaa atgagctccg cagagctgac
     5221 cgaaaaactg gttgcggcaa tcaagagcgg caaatacgac accattatct gtaactatcc
     5281 gaacggcgac atggtaggtc acaccggggt gatggaagca gcagttaaag ccgttgaagc
     5341 gctggatcac tgcgtggatg aagttgcgaa agcggttgag tctgtaggtg gacaactgct
     5401 gatcaccgct gaccacggta acgctgaaca gatgcgcgat ccggcaacgg gtcaggcgca
     5461 cacagcgcac accaacctgc cagttccgct gatttacgtt ggcgataaga acgtgaaagc
     5521 ggttgaaggc ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga
     5581 aatcccgcaa gagatgactg gtaagccgct gttcatcgtg gaataatccc tccccatgag
     5641 gggaaaggcg attaatacca tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc
     5701 catcatctac gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc
     5761 ggatgagcgt gaccaactca aatctattca ggccgatatc gccgcgaaag agcgcgcggt
     5821 acgccaaaag caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc
     5881 gatctctgaa gccacccgta agctgcgcga aacgcaaaac acgctcaatc aactcaataa
     5941 acagattgat gagatgaacg cgtcgattgc caaactggag cagcaaaaag ccgcccagga
     6001 gcgcagcctc gccgcacaac tggatgccgc attccgtcag ggcgagcata ccggtattca
     6061 gctgattctc agcggtgaag aaagccagcg tggacagcgt ttacaggctt atttcggcta
     6121 tctcaaccag gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg aagaagtcgc
     6181 catgcagcgt gctgaactgg aagagaaaca gagcgagcaa caaacgctgt tatatgagca
     6241 gcgcgcccaa caggcgaaac tgactcaggc gctgaacgag cgtaaaaaga cgctggcagg
     6301 gctggagtct tccatccagc aaggtcagca acagttgagc gagctgcgcg ccaacgaatc
     6361 ccgtctgcgt aacagcattg cccgtgcgga agccgcggcg aaagcgcgtg cagaacgaga
     6421 agcacgtgag gcccaggcgg ttcgcgaccg ccagaaagaa gcgacgcgta aaggcaccac
     6481 ctacaaaccg actgaaagcg aaaaatcgct gatgtcccgt accggtggtc tgggcgcgcc
     6541 gcgtggtcag gcattctggc cggttcgcgg gccgacgctg catcgctatg gcgagcagct
     6601 acagggtgaa ttacgctgga aagggatggt gattggtgcc tctgaaggta ctgaagttaa
     6661 agcgattgcc gacggtcggg tgattctggc tgactggctg caaggctacg gtctggtggt
     6721 ggtggttgag catggtaaag gcgacatgag tctttatggc tataatcaga gcgcactggt
     6781 gagcgttggt tcgcaggttc gcgcgggcca gccaattgca ctggtgggca gcagtggcgg
     6841 tcagggtcgg ccttcactct atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca
     6901 gccgtggttg ggaagataag ttttgtttcc atttcgtcgt aacgttcttg catttgccgc
     6961 tctgttggcg ctctcctccc ccgtaattgc tggcaaactt gccatcgtca ttgatgattt
     7021 tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc
     7081 tgtattaccc gattcaccga acgccagaga gatggcgacc aaagcgcata acagcggcca
     7141 cgaagtgttg attcatctac caatggcacc attgagtaaa cagccgctgg agaaaaatac
     7201 gctacgcccg gagatgagca gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa
     7261 cgtgccctat gccgtgggga tcaacaacca catgggcagc aagatgacct ctaacctgtt
     7321 tggtatgcag aaagtgatgc aggcgctgga gcgttacaat ctttacttcc tcgacagcgt
     7381 aaccattggt aatacccagg cgatgcgcgc cgcgcaaggc actggcgtga aggttattaa
     7441 acggaaggtg ttcctcgacg attcacagaa cgaagcggac atccgtgtgc agttcaatcg
     7501 cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc attgggcatc ctcacccttc
     7561 aacggtacgc gtgctacaac agatggttta taacctgccg ccagacatta cgctggtgaa
     7621 agccagcagc ttgctgaatg aaccgcaggt tgatacgtct acaccgccga aaaacactgt
     7681 gcccgacaca ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat
     7741 agaacctgtt tatgctaatc gcttctttga agtattaagc gaaagcatca gccagagcac
     7801 gctgatcgtt tacttccagc atcagtggca aggctggggc aaacagcccg aagcggcaaa
     7861 acttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg
     7921 tcgcgccatt gccataagcg gaaagaccac agtaacgcct ggtaacccac cttcacgctg
     7981 cgcacattgg taatcaggcg cttatacata ccggaagtaa atatctcggc aatcatccgt
     8041 tggcgggtaa gaatatccgg ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa
     8101 gttatttgct gatgaaattc cggataaatc gtaattttgt cggcataatt tcgatttaat
     8161 ttctccagca ggcgggtaat cttaatatag tgacgttgat aattaaggtt tttattccct
     8221 tgtctatgca accgactcac tgacgtatta tgcagataat atttatataa tgattgctcg
     8281 gtatatcgcg cacgcagcgc gttaaacatg aattctgttg tccagacaat atcctgatga
     8341 tgtaatccgg caataaattt aatgttattt ttaacaataa catcgcggcg ataaaccccc
     8401 atccagacaa cgtgagtcca acggcgcgaa gaaagcccca tccgcagcca gtccgggccg
     8461 gttaatacac cggttgagcg aaggcgatcg ctggggatgg attgccaggt ttctcccgtt
     8521 tcacgaaaac accagtcagc gttgcactgc gccacgtcga ggtcgtcctc taacgccatg
     8581 gtcatcagcg tttcgtacat ggtgggatag acttcatcgt cagcatcgac aaaagcgaca
     8641 tatttgcccg ttgccacttc aatcccatga ttacgcgcca ccgatgcgcc agcattcgcc
     8701 tgatgcaaca aacgaacgtg cggatagttt tctgcgtaat gctttgctat ttcaacagaa
     8761 ttatccgttg aaccatcgtt aataataatg atttccagag cagtccaggt ttgcgtaatt
     8821 aaagattcca tacaagtgcg gaaatcatcg cccgcattat ataacggaat aataacacta
     8881 agtttattgg tgctgttcat cataaattac cgacgacctc tgaagttatc ctgacgtttt
     8941 acaggctttg tattaagaaa ctattaagcc tgtgagatat tctttttaca ggtgagagag
     9001 tcacttcagg gtagagagat aatgagagca gctatataag agatcagcac aattacgaac
     9061 tctttattac actgacagga ttttacatat ttaacagatt atcctcagat aaaagggctg
     9121 gaataccagc ccttgttcgt gttaatccca gctcagaata actttcccgg actggcccga
     9181 acgcatagcg tcaaagccct tctggaaatc atcgatagag aaacgatggg taatgatcgg
     9241 cgagagatcg aggccagact gaatcagcgc cgccatcttg taccaggttt caaacatctc
     9301 acgaccgtaa atgcctttaa tgaacaagcc cttaaagatc acttttgtcc agtctatcga
     9361 catgtcagaa ggtggaatac ccagcatcgc aatacgaccg ccgtggttca tggtgtcgag
     9421 catggtacga aacgctggcg gcgcaccgga catttccaga ccgacatcaa agccttcggt
     9481 catgcccagt tctgccatca catcattaag gttttcttta gcgacgttga ccgcacgggt
     9541 gatacccatt ttgcgcgcca gttcgagacg atattcatta acatcagtga tcaccacatt
     9601 gcgtgcacca acgtgtttcg ccaccgccgc agccataata cctatcggac ctgcaccaga
     9661 gaccagcaca tcctcgccaa ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa
     9721 gggatcaaaa atggaagcca ggtcgtcgga gatattgtcg ggaattttga aagcgttgaa
     9781 cgccggaatc accagatatt cggcgaagca gcccggacgg ttaacgccga cgccgatcgt
     9841 gttgcggcac agatgggtac gaccgccacg acagttgcga cagtgaccgc aagtgatatg
     9901 accttcgcca gaaacgcgat cgccaatctt gaagcctttc acttcctgac caatacctac
     9961 cacttcaccg acatattcat ggcccacgac catcggcacc gggatggttt tttgcgacca
    10021 ctcatcccag ttatagatgt gaacgtcagt cccgcagatg gctgttttac ggattttaat
    10081 cagcagatcg ttatgcccga gttccggtac aggaacgtcg gtcatccaga tgccctcttc
    10141 cgctttcagt ttggataacg ctttcatctc acatcctcag gcgataacgc ccagttgttt
    10201 accaatacgc gtgaatgctt ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga
    10261 catctgggta cgaatacgcg cctgaccttt cggaacgacc ggatagaaga aaccggtgac
    10321 gtaaatgccc tctttttgca gctcacgggc aaatttctgc gccactaccg catcaccaag
    10381 catgaccgga ataatggcgt gatcggctcc cgccagggta aagcccgccg ccgacatttg
    10441 ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc agctcgctac ccgcttcgac
    10501 catctccagt actttgatgg atgcggcaac aatggccggt gccagcgagt tggagaacag
    10561 atacggacga gaacgctggc gcagccactc aaccacttct ttgcgcgccg cagtataacc
    10621 accagaagcc ccgcccagcg ctttaccaag cgtaccggtg ataatatcga cccggcccat
    10681 cacatcgcag tattcatggg aaccacgacc gttttcaccg acaaaaccga ccgcgtggga
    10741 gtcgtctacc atcaccaggg catcgtattt atctgccaga tcgcaaacgc ccttcagatt
    10801 ggcaatcacg ccgtccatcg agaacacacc atcggtggca atcagcacat gacgcgcacc
    10861 ggcttcacgc gcttctttca gacgagcttc aagctcctgc atatcgttgt tggcatagcg
    10921 atagcgttta gctttacaca gacgcacacc atcaataata gacgcgtggt tcagtgcgtc
    10981 ggagataatg gcgtcttccg cgcccagcag cgtttcaaac aggccaccgt tagcatcaaa
    11041 gcaggaagag tagagaatcg catcttccat ccccaggaat gccgccagtt tttgttcaag
    11101 ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc gaagccatgc cgaaaccgtg
    11161 agaatccatt cccgcctttg ccgccgcaat cagatccgga tgattcgcca gcccgagata
    11221 gttattggca caaaagttaa tgacgtggct tccatcagcc acagtgatat ctgcttgctg
    11281 cgcagacgta ataatgcgct cttctttaaa caacccttcc gcccgtgcgg tttccagatc
    11341 gttggttaac tgctgataaa attctccacg cattgcgatt ctccagacag ggcaaattcc
    11401 agcacatatt acccaaactt ataggtcgcg acgagatgac gcgttaacac ttctgcaaaa
    11461 ttcaggataa ataacgtgta tcttgatgtc tctcaacacc tacagatagt agaaaaactg
    11521 tacagttttg gctgagcata atgatgagtg attttactgc attgatataa aaagaaaaaa
    11581 tgatgccaaa acatcatttt tcctatctca gttggcatca aaacgctacc cactaatggc
    11641 gggaaaccta ttttcttgaa aggaaaattc tcagcgcctg catttttgcc cctaaagtgt
    11701 tactcctgaa gcagcgaaac aaatcgaacc attttcctct ccccagatag tttaggttga
    11761 agagaccgga acgtttgtaa tagcacatga caaaaatgcc ctggtcatcg tccacaatgt
    11821 tattgtttag tgttattttt tgactttcca gaactaattt atagaattct ttccatttat
    11881 actgtgaacc aacaattgcg cccccaataa tatatacatg attgccaatc atgaaatcaa
    11941 aagcctgttg ttgagatgta acagtaagcc ctttttttat agtgaaaaga tgcatcttat
    12001 tttcatcaaa tgggaaatcc caaattttca atcctcgagt cacatttggc ttacggcaat
    12061 agccaaaatc tatccacgca acaagaggcg ttttcactaa gcccatgttg atagctttat
    12121 ttacaaaata ggccttaagg ttacatacca aaacatactc tggtgaccag tactctgggt
    12181 tttttaattg tcgaggctca agtctatttg taaacgactc atctttttga attttttcga
    12241 tacgacttct gatatgtcta aatttctttt ttatatcgat agcgataacc gttgttggtt
    12301 ttccgttgcg aatcgcttca acccttggtt tcaaatcggg agaagtgaaa ataatcatct
    12361 cattttcaag tacggccaga cgctcaaagt aactaaaata aacatcaact gaacgagcta
    12421 gtttttcacg gaacccttta ttagcagtcc aatcccctct accaatgtca aaatatgcag
    12481 tgataattgt ggtggatgat ttcattacta ataagaccat taatataaaa aaataattat
    12541 atcataaata ttaacagaat gtatactgta tattatccat tcaaatgctt ccgttattta
    12601 ccatacatcg gaatattgat actaaagcac tattcacgtg caaaaccaac atccgccatg
    12661 aaggactagc taaaacccaa actagtttgt tgcaattagc atccttgcat ctctatgtaa
    12721 agggctgaag ggattcggat gtgatggtat gattacagac attcatgtct gagattgtct
    12781 ctgactccat aattcgaagg ttacagttat gatcatcgtt accggcggcg cgggctttat
    12841 cggcagcaac atcgttaagg ccctgaatga taaaggcatc accgatattc tggtggtgga
    12901 caacctgaaa gacggcacca agtttgtgaa cctggtggat ctggatatcg cggactatat
    12961 ggataaggaa gacttcctga tccagattat ggctggcgaa gagttcggcg atgtcgaagc
    13021 gattttccac gaaggcgcat gctcttccac caccgagtgg gatggcaagt atatgatgga
    13081 taacaactat caatactcca aagagctgct gcactactgt ctggagcgcg aaatcccgtt
    13141 cctgtatgca tcttccgcgg ccacctacgg cggacgcacc tccgacttta ttgaatcccg
    13201 cgagtacgaa aaaccgttga acgtctacgg ttactcaaaa ttcctgtttg atgaatatgt
    13261 tcgtcaaatc ctgcctgaag cgaactcgca gattgttggc ttccgttatt tcaacgttta
    13321 tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc gctttccatc tcaacaccca
    13381 gcttaacaac ggtgaatcac cgaagctgtt tgaaggtagc gagaacttca aacgcgactt
    13441 cgtctatgtg ggcgacgtgg ctgatgtgaa tctgtggttc ctggaaaatg gcgtttccgg
    13501 catcttcaat ctcggtactg gtcgtgcgga atccttccag gctgtagctg atgctacgct
    13561 ggcttatcac aagaaaggcc agatcgaata cattccgttc ccggataaac tgaaaggccg
    13621 ctatcaggcg ttcactcagg cagatctgac aaatctgcgc gcggcgggtt acgacaaacc
    13681 gttcaaaacc gttgctgaag gtgtaacgga atacatggcc tggctgaatc gcgacgcata
    13741 agagctctgc atgaaaatac tggtgatcgg cccgtcttgg gttggcgaca tgatgatgtc
    13801 gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag gcgataatcg atgtgatggc
    13861 accggcatgg tgccgtccat tattatcgcg gatgccggaa gttaacgaag ctattcctat
    13921 gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc aaactgggtc atagtctgcg
    13981 tgaaaagcgc tacgaccgcg cctacgtctt acccaactcc ttcaaatctg cattagtgcc
    14041 tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc gagatgcgct acggtttact
    14101 caacgatgta cgcgtgctcg ataaagaagc ctggccgcta atggtggaac gctatatagc
    14161 gctggcctat gacaaaggca ttatgcgcac agcacaagat ctgccgcagc cattgttatg
    14221 gccgcagttg caggtgagcg aaggtgaaaa atcatatacc tgtaatcaat tttcgctttc
    14281 atcagaacgt ccgatgattg gtttttgccc gggtgcggag tttggtccgg caaaacgctg
    14341 gccacactac cactatgcgg agctggcaaa gcagctgatt gatgaaggtt atcaggtggt
    14401 tctgtttggc tcggcgaaag atcatgaagc gggcaatgag attcttgccg ctttgaatac
    14461 cgagcagcag gcatggtgtc ggaacctggc gggggaaaca cagcttgatc aagcggttat
    14521 cctgattgca gcctgtaaag ccattgtcac taacgattct ggcctgatgc atgttgcggc
    14581 ggcgctcaat cgtccgctgg ttgccctgta tggtccgagt agcccggact tcacaccgcc
    14641 gctatcccat aaagcgcgcg tgatccgttt gattaccggc tatcacaaag tgcgtaaagg
    14701 tgacgctgcg gagggttatc accagagctt aatcgacatt actccccagc gcgtactgga
    14761 agaactcaac gcgctattgt tacaagagga agcctgacgg atgcgggttt tgatcgttaa
    14821 aacatcgtcg atgggcgatg ttctccatac gttgcccgca ctcactgatg cccagcaggc
    14881 aatcccaggg attaagtttg actgggtggt ggaagaaggg ttcgcacaga ttccttcctg
    14941 gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt cgctggcgta aagcctggtt
    15001 ctcggccccc ataaaagcgg aacgcaaagc gtttcgtgaa gcgctacaag cagagaacta
    15061 tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg gcgctggtga cgcgtctggc
    15121 gcatggcgta aagcatggca tggactggca aaccgctcgc gaacctttag ccagcctgtt
    15181 ttacaatcgt aagcatcata ttgcaaaaca gcagcacgcc gtagaacgca cccgcgaact
    15241 atttgccaaa agtttgggct atagcaaacc gcaaacccag ggcgattatg ctatcgcaca
    15301 gcattttctg acgaacctgc ctacagatgc tggcgaatat gccgtatttc ttcatgcgac
    15361 gacccgtgat gataaacact ggccggaaga acactggcga gaattgattg gtttactggc
    15421 tgattcagga atacggatta aacttccgtg gggcgcgccg catgaggaag aacgggcgaa
    15481 acgactggcg gaaggatttg cttatgttga agtattgccg aagatgagtc tggaaggcgt
    15541 tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg gatacggggt taagccattt
    15601 aacggcggca ctggatagac ccaatatcac ggtttatgga ccaaccgatc cgggattaat
    15661 tggcggatat gggaagaatc aagtagagtg tcgttctaca agtatgtctc ttgcagattt
    15721 gccagctcaa accgtttttc aaaatttaaa tcttgaaata ataatcaata agttgacatc
    15781 ggagataaga tgacctcaac attatttttc tctctcgaga aaaaaaactg gatagcgtac
    15841 tggaacagag ctctcgtatt cttatttatt accacctatt ttttgggtgg gattacaagg
    15901 tataaacatc ttattgttat tcttatgaca ataacgacaa tcgtctatct ctgcaaacgg
    15961 ccaaaacact atctatcact atttaaaaca tttctttttg gtagtgttgc catattaact
    16021 attgctgcat tgctgtcact tcttcaatct ccagatgcag gtgctagcat gaaggaaatt
    16081 ttcaaagcta tcattgagaa tactttacta tgcacaatag caataccggt catattgaga
    16141 gacgaaaaaa gagaaaatat cgaaaaaatc gttttcttct catttattag tgcgttgagc
    16201 ttacgctgtt tttctgaatt gattgcctat tataaggact atcaacaagg gataatgcca
    16261 ttcgcagatt atagacaccg tagcatttct gactcgatgg tctttttatt ccctgcattg
    16321 ttaaatctct ggcttattaa atcagcaaaa taccgcattt cttttgtggt tctaagcttt
    16381 atttttattt ttctgatatt aggaacttta tccagagggg cctggctttc cgtgttagtc
    16441 attggattaa tatggattct gatgtttaaa caatggaagt tactattagt aggagtaatg
    16501 gtttccatca ttgcattgtc ggttattttc acacataagg agatgaccgc aaagctaacg
    16561 tataaacttc aacaaactaa tagttcttat cgctatgcaa atggtactca aggcagcgca
    16621 ctcgatctaa tattagaaaa tcctgttatt ggttatggtt acggtaacgt tgcatataaa
    16681 gatgtctata ataaacgtgt cgttgattat ccagaatgga cctttagaca atcaataggg
    16741 ccacataatt ttgcgctatt catctggttt ggcactggtt tattagggct ggtaagtctt
    16801 atgatgctat actgtgcaat attgaaagag tgtataaaaa atggcgtcca gaataaatac
    16861 cgctcaccat gtaatgcata ttatataatc ttactatctt ttataggtta ttttgttatc
    16921 cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg tttacgccgg cttattatta
    16981 gcg
//
LOCUS       DAZGCQ010000103        16588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000103 DAZGCQ010000000
VERSION     DAZGCQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.103"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..189)
                     /locus_tag="ACHK07_003883"
     CDS             complement(37..189)
                     /locus_tag="ACHK07_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8104157.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHK07_003884"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHK07_003884"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8104158.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHK07_003885"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHK07_003885"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO8104159.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHK07_003886"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHK07_003886"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO8104160.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAEKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVESEVHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHK07_003887"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHK07_003887"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO8104161.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHK07_003888"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHK07_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8104162.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDFPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHCE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYWAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHK07_003889"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHK07_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8104163.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHK07_003890"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHK07_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO8104164.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHK07_003891"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHK07_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8104165.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTISVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIDADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHK07_003892"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHK07_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO8104166.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAAELRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHK07_003893"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHK07_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104167.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHK07_003894"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHK07_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO8104168.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHK07_003895"
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHK07_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8104169.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13467..14744
                     /gene="yqcE"
                     /locus_tag="ACHK07_003896"
     CDS             13467..14744
                     /gene="yqcE"
                     /locus_tag="ACHK07_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO8104170.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVLQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
     gene            14771..16249
                     /gene="ygcE"
                     /locus_tag="ACHK07_003897"
     CDS             14771..16249
                     /gene="ygcE"
                     /locus_tag="ACHK07_003897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO8104171.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLMHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3938 a         4377 c         4166 g         4107 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac
      121 catcagcgtg aatcctagta ctgtgcaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatgc tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgtaacc cacattccct
      481 ttttaagcca aagaaacgcg tttcttcttc cgccatgccg ggttcccatt tacgggttgt
      541 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
      601 cttttgcaga tactgatata tcgttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcgttaag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gttttccgta
      841 gcgtgcttcc tgccaggctg cgctttcggt agcacggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagataac ccgtatcggt
      961 gaggatcacc gggatatccg ggcgaatctg attcaccaga tgcaggctga ccgccgcctg
     1021 aatgccaaag ctggaagaaa gcacatattc accgggcaga ttatccagcg cccaggctac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatacg gttgccgcca agatgcaggt tgtagcgacc cggcgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaacgag acgcaggcca
     1681 ttgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggcacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc acctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatctccgc gtccggtaaa ctcatacggg cgaatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttccgcc accgccagcg tatgctccag cggcagatag ccaaactcac
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 acaggttaaa gcccaccagc ttgccgtttt cggcgatcgc cacgaagttc atgtcgttgg
     2281 cgtgcagatc gatatcgttc tgtggcggga tcactaccgt ggttttgaat ttacgcggca
     2341 ggtaggtctg gccgaggatc ggttcttcat cagtggtggc gactttttcc tgatcgagcc
     2401 agatctccgc atacgcgcgg gtacgcggca gcagatgttc agagattttc ttcgcccact
     2461 cgtacgcttc tgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agcggtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actggaatgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttatcgat cgcctgccac tgtttggtgg
     2701 tgataactcc acccggcaga cggcagcgca acagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccgt cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg atcgcgcgac caggcgagat cgatccgcgt
     3241 cagcacaccc tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
     3301 atgcggattg ccaaagaaca gccagttttt acctggcgct tcgtcagcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcct atcataatca ccggggtttc
     3421 cgggttagcg ggcagacgga agttgtcgtt atgttcgata aagacgcgga cttcaccctc
     3481 ttcttccacg cggtccgcga ggaagctgga ggcaccaccc gcacgggcgc ggccttcaat
     3541 gtcataacga acgacaccaa cggtgacgtg cacttcactt tccacttcag cctgcgatga
     3601 ggcgatggaa tagaggcgcg gcgtcagcgg gcgcagcagg ttaatcagcg cttcagcatc
     3661 aagctgtgcc ggggagaaac gcaccatgtc aacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttca ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acacccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaaccga aaggctcgct accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccac gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acgtcggcgt cgacacggtc gagcaggcgt tcaccgccca gttccgccag
     4261 cttgctgtcg aaatctttcc cggactggca gaaaaattca taagaactat cgccgaggct
     4321 aaacacggca aaagcggtat tttccagctt cggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagtttttct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaagca gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaaa caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaagcca
     5281 acttatgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccgacc
     5341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgcaccgct ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tcaggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctccctca gtttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
     5881 tcgaatgtat ccccggagcg acggtggatg cactgtatga agaaaatggc agagtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgaacgt cacggattag tgactcgtcc tgctggcgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcggaat ctgtgatgac tttcccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctggc tcgctttaaa acgtatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccagtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttacg cagttgcgtc aataccggaa tctccgtgcg cggcatggat atggcgctga
     6481 ctggcgcaca ggcggcggca caaacgctga taagcgcctg ccagcactgc gagccgcaaa
     6541 atctgtttcc gctttatcat cacaacgtcg agcgcagcct gctgtgggat gttctacagc
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtattgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc acggcctgtg gcatctggcg ggcgacgtta
     6781 tcaggagtct gcgatgtctg tagcccgtaa tctctggcgc gctgctgatg cgccacatat
     6841 tgttccggct gactccgttg agcgccagac ggcagaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgtatt gactatcgca gttgcctgga
     6961 gtgtggcacc tgccgtttgc tgtgcgacga atcaacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgttttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcga tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg catgcccaaa gtgctgggct
     7501 gggtgacgga aaaaatccgc caaccgctga ttgctggcgg gctggtatgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggagtct
     7621 ggacgttagc gaaaaaattg ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttaa cgcgtcttat
     7741 caggcctaca ggtccccagt ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt caatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgctgcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gtgcccgact gtagcctact tcggctccca gcttttctgc cagcatagca
     8101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa ccagccagtc cggaagcgca
     8221 cccgggacaa tgttcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt atggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaactac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gtgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgcgctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccacg tcgttgctct gctcttgcca
     8761 gttgccgaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caacccatgt tcagtacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gacgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg tgcaaagcgc agttctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca acgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggcg gcagcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaacatccgg tccgctatta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagtgc tttggtttct
     9481 ggtgcccata gccaggtcac gacaaaacca actaacaaca ctgccgccag aatcaataag
     9541 gtaacctgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gcgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagcagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaaca
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga gtaatcgcct
    10381 cccagaccaa tgccgatcaa aatgcgcagc ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgcagga acgaagcgag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa gaaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagttgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaattc gacagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccatcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc gttaggatcg aattgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag cttgctccag tgaacgcgat gtttaccaat gccgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcgcaga tgattttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagttgacaa cgttgtagtc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggagtgac cacccagcat agtgatgtcg
    11161 tcggcgtgcg ggaactcggt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatttgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agcttgctgt ccacgcgttg gcattgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac gcattttccg
    11461 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gatttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcacaatgc accttcgttg ccgatgatga tgtgacgaat gtccgggcca
    11701 gccgcgcggc gtggcacgtt tttaatacgt gtgacggtgc catcagccaa tacggcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc gttttccagc acctccagcg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggaacc gtcgagcacc
    12001 accgagtttt ctacaacagt ttccagccca ccttcggtgg cggaagcacc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggatacttg ctctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa tgccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 ggttagcgca ctaaataacc gccatcgacc actaataaat gcccgttgac ataattcgat
    12421 gcctgacttg cgagaaacac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcgata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaatccggca agagcatgtt tagtagcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taatgatttt accgcttttc
    12721 tgcgggatca taatttttgc cgcttcgtag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcattg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt taaccagaat atcaactgta ccgaaacgct cacagcaggc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctga
    12961 ttttcaatca tttcctttgt ttcaccgtta tctttgacaa aacttggaat aaagacattt
    13021 gcgcctgctt tggccaacgc catggcaaat gcctggccta aaccgctatt cccgccggta
    13081 acaattgcgg ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc
    13201 gagagggaag ttacctttct cattatctct ggtaactgga gataattttt agcataagga
    13261 cataactgct aatgtgatcg taatcacagt gtgatattcg cattatcaaa tagttttaaa
    13321 aagcagaagc gtgattactc tcacacaaca cctctgcttt ccatgttatt tcttattgca
    13381 attactagag acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt
    13441 ttgtctatta caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc
    13501 tcgcgataat tagttttagc ggcggcgtta gttttgacct ggcttattta cgttatattt
    13561 atcaaattcc catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga
    13621 gtacctttgg tattgcagct attattcttt atgcccccag cggcgttatt gccgataaat
    13681 tctcacatcg caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa
    13741 tggcaacgta tccaccgctg tgggtaatgc tctgtattca ggtcgccttt gcgataacga
    13801 cgattttaat gctgtggtcg gtgtcgatta aagccgcctc attgctgggc gatcatagtg
    13861 agcaagggaa aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg
    13921 cggtgtttac catgtgggtc ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa
    13981 cggtcattat catctatagt gtggtttaca tcttgttggg gattctgtgc tggttttttg
    14041 ttagcgataa caacaacctg cgcagtgcca ataacgaaga aaaacaatca ttccagctta
    14101 gcgacatcct ggccgttttg cgcatcagca ccacctggta ttgcagcatg gtgatttttg
    14161 gtgtcttcac catctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtatg
    14221 gcatgtcgct ggtggcggcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc
    14281 tgtgcggccc acttggcggc attatcacca cctacagtaa agtgaaatcc cctacccgcg
    14341 tgctccaaat cctttccgta ctcggcctgc tggcgttaac tgccctgctc gtcacgaact
    14401 ctaacccgca atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt
    14461 acgcctcacg cgggctgtat tgggcctgcc ctggcgaagc aagaacaccg tcttacatta
    14521 tgggcaccac ggtaggtatt tgttcggtga taggattcct gccggatgtc ttcgtttacc
    14581 caattatcgg ccactggcag gacaccctgc ccgcagcaga agcctaccgc aatatgtggc
    14641 tgatgggcat ggcggcgctc ggcatggtga tcgtctttac ctttttgttg ttccaaaaaa
    14701 ttcgtactgc tgatatcgcc cccacaacgg ctaacagcaa gtaagccaga aaaattacag
    14761 gagaaaaatc atgtcgaaga aatacatcat agggattgat ggcggaagtc agagcacaaa
    14821 agtggtgatg tacgatctgg aaggtaacgt ggtttgcgaa ggcaaaggtt tattgcaacc
    14881 gatgcacacg ccagatgccg ataccgcaga acatcctgac gacgatttat gggcatcatt
    14941 atgttttgcc ggtcacgatt tgatgagtca gtttgccggg aataaagaag atattgtcgg
    15001 tattggtctg ggatccatcc gttgctgccg tgcgttattg aaagccgatg gcacgccagc
    15061 tgcgccattg attagctggc aggatgcacg cgtaacgcgt ccctacgaac acactaatcc
    15121 tgacgtggca tatgtcacct ctttttcggg ttatctgatg catcgcttaa ccggcgagtt
    15181 taaagacaat atcgccaact attttggtca gtggccggtg gattataaga cctgggcatg
    15241 gagtgaagat gctgcggtaa tggataagtt taatatcccc cgtcatatgc tgtttgatgt
    15301 gcaaatgcct ggcaccgtcc tcggacatat cacaccacaa gccgcactgg cgacacattt
    15361 cccggcagga ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggggc
    15421 cggattactg gatgatgaaa ctgcggtaat ttctcttggc acttacatcg cattgatgat
    15481 gaacggcaaa gcactgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc
    15541 gcaaacattg ctgtatgaag gttacggtat tcgcaaaggc atgtggacgg tgagctggct
    15601 gcgcgacatg ctaggcgagt cgttaattca ggatgccagg gcgcaggatc tttcaccgga
    15661 agatttactc aacaaaaaag cttcttgtgt gccgccaggc tgtaatggtc tgatgacggt
    15721 gctggactgg ctgaccaatc cgtgggaacc gtacaaacgc gggattatga tcggctttga
    15781 ttccagcatg gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct
    15841 gaagaacaat tacgacaata tgtgtaatga aatgaatcac tttgcgaagc atgtaatcat
    15901 tactggcggc ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct
    15961 tccggcacga cgtaacgcca ttaacggttg tgcaagtctg ggggcagcga ttaatacagc
    16021 ggtaggtctg gggctatacc cggattacgc aacggctgtc gataaaatgg ttcgcgtgaa
    16081 agatatcttt atgcctgttg agagcaatgc caaacgctac aacgcgatga ataaaggcat
    16141 tttcaaagat ttaaccaaac acactgatgt gattctgaaa aaatcgtatg aagtgatgca
    16201 tggggaattg gggaatgcgg attcgatcca gagctggtcg aatgcgtaag tgaggaagac
    16261 cgggcgggaa actgcccggc ctgaacatac ctgaatggtt atccccgctg gcgcggggaa
    16321 cataagttcg acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga
    16381 acataataaa aactttaact agttcggttt atccctgttt ttacaaggca tattaactac
    16441 actcgatgtc tttagaataa aagacaatac gaatcgcact ttatcaattt tcacttaaag
    16501 tatgaactgg atatctttat tcataagaaa tattcatcaa cagcgtatag aggcggttat
    16561 gtaaaaccac tcattagcct caaaacaa
//
LOCUS       DAZGCQ010000104        16570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000104 DAZGCQ010000000
VERSION     DAZGCQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.104"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            270..485
                     /gene="essD"
                     /locus_tag="ACHK07_003898"
     CDS             270..485
                     /gene="essD"
                     /locus_tag="ACHK07_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO8104172.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            485..982
                     /gene="rrrD"
                     /locus_tag="ACHK07_003899"
     CDS             485..982
                     /gene="rrrD"
                     /locus_tag="ACHK07_003899"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO8104173.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1199..1381
                     /gene="rzoD"
                     /locus_tag="ACHK07_003900"
     CDS             1199..1381
                     /gene="rzoD"
                     /locus_tag="ACHK07_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO8104174.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1472..1765)
                     /gene="iss"
                     /locus_tag="ACHK07_003901"
     CDS             complement(1472..1765)
                     /gene="iss"
                     /locus_tag="ACHK07_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO8104175.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2125..2319)
                     /locus_tag="ACHK07_003902"
     CDS             complement(2125..2319)
                     /locus_tag="ACHK07_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8104176.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            2708..3253
                     /locus_tag="ACHK07_003903"
     CDS             2708..3253
                     /locus_tag="ACHK07_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO8104177.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3228..5153
                     /locus_tag="ACHK07_003904"
     CDS             3228..5153
                     /locus_tag="ACHK07_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8104178.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5150..5356
                     /locus_tag="ACHK07_003905"
     CDS             5150..5356
                     /locus_tag="ACHK07_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO8104179.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5353..6954
                     /locus_tag="ACHK07_003906"
     CDS             5353..6954
                     /locus_tag="ACHK07_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8104180.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6935..8254
                     /locus_tag="ACHK07_003907"
     CDS             6935..8254
                     /locus_tag="ACHK07_003907"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8104181.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLAAGEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGIMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATALQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8264..8596
                     /locus_tag="ACHK07_003908"
     CDS             8264..8596
                     /locus_tag="ACHK07_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO8104182.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8651..9676
                     /locus_tag="ACHK07_003909"
     CDS             8651..9676
                     /locus_tag="ACHK07_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8104183.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9718..10113
                     /locus_tag="ACHK07_003910"
     CDS             9718..10113
                     /locus_tag="ACHK07_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8104184.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            10125..10478
                     /locus_tag="ACHK07_003911"
     CDS             10125..10478
                     /locus_tag="ACHK07_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8104185.1"
                     /translation="MADFDNQFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10490..11068
                     /locus_tag="ACHK07_003912"
     CDS             10490..11068
                     /locus_tag="ACHK07_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104186.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11065..11460
                     /locus_tag="ACHK07_003913"
     CDS             11065..11460
                     /locus_tag="ACHK07_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8104187.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11468..12208
                     /locus_tag="ACHK07_003914"
     CDS             11468..12208
                     /locus_tag="ACHK07_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104188.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            12224..12646
                     /locus_tag="ACHK07_003915"
     CDS             12224..12646
                     /locus_tag="ACHK07_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8104189.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12628..13062
                     /locus_tag="ACHK07_003916"
     CDS             12628..13062
                     /locus_tag="ACHK07_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8104190.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13055..15616
                     /locus_tag="ACHK07_003917"
     CDS             13055..15616
                     /locus_tag="ACHK07_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8104191.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSVSGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHADLLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMTGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15613..15942
                     /locus_tag="ACHK07_003918"
     CDS             15613..15942
                     /locus_tag="ACHK07_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104192.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15942..>16570
                     /locus_tag="ACHK07_003919"
     CDS             15942..>16570
                     /locus_tag="ACHK07_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8104193.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCL"
BASE COUNT         3871 a         4126 c         5025 g         3548 t
ORIGIN      
        1 tggtggtgtt ttgaacactc ggtagcattc tcataaatat cattcagtgg tttacgtacg
       61 taaaaaattg gttatgctgt taagagtggt tacttcgtca cacagcttaa acccgccgtc
      121 gagctggttt ttccattttt tgagtctcga tattagctga taactcaata cctaagttat
      181 tcactgactc cgagtctgtt acgtttctgc ttttttgcga tacgttgtat tccctcaatt
      241 tacacccgct ttgtctgcga ggtggggtta tgaaatccat ggataagtta acaacgggtg
      301 tcgcctatgg cacctcagca ggtagtgccg gttactggtt tttacagctg ctcgataaag
      361 tcacgccctc acagtgggca gcaataggtg tgctgggtag cctggtattt ggcctgctga
      421 cgtacctgac aaacctttat ttcaagatta aagaagataa gcgcaaggct gcgagaggtg
      481 aataatgcct ccatcattac gaaaagtcgt tgctgctgct attggtggcg gagcaattgc
      541 tatagcatca gtgttaatca ctggcccaag tggtaacgat ggtctggaag gtgtcagcta
      601 cataccatac aaagatattg ttggtgtatg gactgtatgt cacgggcata caggaaaaga
      661 catcatgctc ggtaaaacgt ataccaaagc agaatgcaaa gcactcttga ataaagacct
      721 tgccactgtc gccagacaaa ttaacccgta catcaaagtc gatataccgg aaacaacgcg
      781 cggcgctctt tactcattcg tttacaacgt gggtgctggc aatttcagaa catcgacgct
      841 tcttcgcaaa ataaaccagg gcgatatcaa aggcgcatgt gatcagctac gtcgctggac
      901 atatgctggc ggtaagcaat ggaaaggtct catgactcgt cgtgagattg agcgtgaaat
      961 ctgtttgtgg ggtcagcaat gaacagagta accgcgatta tctccgctct ggttatctgc
     1021 atcatcgtct gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa
     1081 gcccagcgcg acaaaaatgc cagagaactg aagctggcga acgcggcaat tactgacatg
     1141 cagatgcgtc agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga gttagctgat
     1201 gcgaaagctg aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg tcggttgcac
     1261 atcaaagcag tctgtcagtc agtgcgtgaa gccaccaccg cctccggcgt ggataatgca
     1321 gcctcccccc gactggcaga caccgctgaa cgggattatt tcaccctcag agagaggctg
     1381 atcactatgc aaaaacaact ggaaggaacc cagaagtata ttaatgagca gtgcagatag
     1441 agctgcccat atcgatgggc aactcatgca attattgtga gcaatacaca cgcgcttcca
     1501 gcggagtata aatgcctaaa gtaataaaac cgagcaatcc atttacgaat gtttgctggg
     1561 tttctgtttt aacaacattt tctgcgccgc cacaaatttt ggctgcatca acagttttct
     1621 cctgtccaat tcccgaaacg aagaagtgat gggtgatggt ttcctttggt gttactgctg
     1681 tcggtttgtt tccaacagta aacgtctgtt gagcacatcc tgtaataagc attgccagag
     1741 cggcagaaaa caacattttt ttcatcttat tatcctgcat tgttaaaaac ggcagaatcc
     1801 tatgtgacaa caattaaacg atagttaaat ggattgatga aaattaaaac tatataggtg
     1861 gatgctcagc ctattggagg aggggggcac tcagaatcct gtggaatgaa ataaaccgct
     1921 ctatctgtcc attacccttt tagctgcgct gtatcgtcgc cgtattcccg cattaaccat
     1981 gaccgtagcc cgacggggaa ttccttctgc gtgagtgtgc gggaataatc aaaaacgatg
     2041 cacaccgggt tttactgtgc tgacagacgc agggttaccc tcatagtcgc ttttccggtg
     2101 cgatggtgga agaaaccggg atgtttattc atcatcactt tggattgatg tatatgctct
     2161 cttttctgac gttagtctcc gacggcaggc ttcaatgacc caggctgaga aattcccaga
     2221 ccctttttgc tcaagagcga tgttaatttg ttcaatcatt tggttaggaa agcggatgtt
     2281 gcgggttgtt gttctgcggg ttttgttctt cgttgacatg aggttgcccc gtattcagtg
     2341 tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac
     2401 gatacctgcg tcataattga ttatttgacg tggtttgatg gcgtagatgc acgttgtgac
     2461 atatagatga taattattat cattttgcgg gtcctttccg gcgatccgac aggttacggg
     2521 gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt
     2581 cttctttgcc gtaacttaat gtttttattt aaaacaccct ctgaaaagaa aggaaacgac
     2641 aggtgctgaa aacgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga
     2701 atgaacaatg gaagtcaaca aaaagcagct ggctgacatt ttcggcgcga gtatccgtac
     2761 cattcagaac tggcaggagc agggaatgcc cgttctgcgg ggtggtggca agggtaatga
     2821 agtgctttat gactctgccg ccgccataaa atggtatgcc gaaagggatg ctgaaattga
     2881 gaacgaaaag ctgcgccggg aagttgaaga actgcggcag gccagcgaga cagatctcca
     2941 gccagggact attgagtacg aacgccatcg acttacgcgt gcgcaggccg acgcacagga
     3001 gctgaaaaat gccagagact ccgctgaagt ggtggaaacc gcattctgta ctttcgtgct
     3061 gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg atccccctgt cggtgcagcg
     3121 gcgttttccg gaactggaaa accgacatgt tgatttcctg aaacgggata tcatcaaagc
     3181 catgaacaaa gcagccgcgc tggatgaact gataccgggg ttgctgagtg aatatatcga
     3241 acagtcaggt taacaggctg cggcattttg tccgcgccgg gcttcgctca ctgttcaggc
     3301 cggagccaca gaccgccgtt gaatgggcgg atgccaatta ctatctccca aaagaatccg
     3361 cataccagga agggcgctgg gaaacactgc cctttcagcg ggccatcatg aatgcgatgg
     3421 gcagcgacta catccgtgag gtgaatgtgg tgaagtctgc ccgtgtcggt tattccaaaa
     3481 tgctgctggg tgtttatgcc tactttatag agcataagca gcgcaacacc cttatctggt
     3541 tgccgacgga tggtgatgcc gagaacttta tgaaaaccca cgttgagccg accatccgcg
     3601 atattccgtc gctgctggcg ctggctccgt ggtatggcaa aaagcaccgg gataacacgc
     3661 tcactatgaa gcgtttttcc aatggtcgtg gcttctggtg cctgggcggt aaagcggcaa
     3721 aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga tgaacttgct gcctttgatg
     3781 aggatattga acaggaaggc tctccgacgt tcctgggtga caagcgtatt gaaggctcgg
     3841 tctggccaaa gtccatccgt ggctccacgc ccaaagtgag aggcacctgt cagattgagc
     3901 gtgcagccag tgaatccccg cattttatgc gttttcatgt tgcctgcccg cattgcgggg
     3961 aggagcagta tcttaaattt ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg
     4021 atgacccctc cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg
     4081 agctggactt tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg
     4141 gcattctctg gttttcgtca tccggtgaag agattgagcc acctgacagt gtgacctttc
     4201 acatctggac agcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga
     4261 aaacgaaagg ggatacggga aaacgtaaaa ccttcgtaaa caccacgctc ggtgagacgt
     4321 gggaggcgaa aattggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt
     4381 attcagcgcc cgttcctgac cgtgtggctt acctgaccgc cggtatcgac tcccagctgg
     4441 accgctacga aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgacc
     4501 ggcagattat tatgggccgc cacgacgatg aacagacgct gctgcgtgtg gatgaggcca
     4561 tcaataaaac ctatacccgc cggaatggtg cagaaatgtc ggtatcccgt atctgctggg
     4621 atactggcgg gattgacccg accattgtgt atgaacgctc gaaaaaacat gggctgttcc
     4681 gggtgatccc cattaaaggg gcatccgtct acggaaagcc ggtggccagc atgccacgta
     4741 agcgaaacaa aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga
     4801 tttataaccg cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact
     4861 tccccaataa cccggatatt tttgatctga ccgaagcgca gcagctgacg gctgaagagc
     4921 aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca
     4981 atgaggcgct cgactgcttt gtttatgcgc tggcggcgct gcgcatcagt atttcccgct
     5041 ggcagctgga tctcagtgcg ctgctggcga gcctgcagga agaggatggt gcagcaacca
     5101 acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca
     5161 ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa aacgggtggc
     5221 aacggtacag aaagacggac ggagagtgga gtttacggcc acttccgtgt ctgacctgaa
     5281 aaaatacatt gcggagctgg aagtgcagac cggcatgaca cagcgacgca ggggacctgc
     5341 aggattttat gtatgaaaac gcccaccatt cccacccttc ttgggccgga cggcatgaca
     5401 tcgctgcgcg aatatgccgg ttatcacggt ggtggcagcg gatttggtgg gcagctgcgg
     5461 gcgtggaacc caccgggtga aagtgtggat gcagccctgt tgcccaactt tacccgtggc
     5521 aatgcccgcg cagacgatct ggtacgcaat aacggctatg ctgccaacgc catccagctg
     5581 catcaggatc atatcgtcgg gtcctttttc cggctaagtc atcgcccaag ctggcgttat
     5641 cttggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     5701 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     5761 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     5821 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     5881 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     5941 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     6001 cagaaatgga catggatacc ccgtgaatta cccggcgggc gcgcctcgtt cattcacgtt
     6061 tttgaacccg tggaggacgg gcagacccgc ggtgcaaatg tgttttacag cgtaatggag
     6121 cagatgaaga tgctcgacac gctgcagaac acgcagctgc agagcgccat tgtgaaggcg
     6181 atgtatgccg ccaccattga aagtgagctg gatacgcagt cagcgatgga ttttattctg
     6241 ggcgcgaaca gtcaggagca gcgggaaagg ctgacgggct ggattggtga aattgccgcg
     6301 tattacgccg cagcaccggt ccgtctggga ggcgcaaaag tgccgcacct gatgccgggg
     6361 gactcactga acctgcagac ggctcaggat acggataacg gctactccgt gtttgagcag
     6421 tcactgttgc ggtatatcgc tgccgggctg ggtgtctcgt atgagcagct ttcccggaat
     6481 tacgcccaga tgagctactc cacggcacgg gccagtgcga acgagtcgtg ggcgtacttt
     6541 atggggcggc gaaaattcgt cgcatcccgt caggcgagcc agatgtttct gtgctggctg
     6601 gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt cagctttcag
     6661 gaagcccgca gtgcctgggg gaactgcgac tggataggct ccggtcgtat ggccatcgat
     6721 ggtctgaaag aagttcagga agcggtgatg ctgatagaag ccggactgag cacctacgag
     6781 aaagagtgcg cgaaacgcgg tgacgactat caggaaattt ttgcccagca ggtccgtgaa
     6841 acgatggagc gccgcgcagc cggtcttaaa ccgcccgcct gggcggctgc ggcatttgaa
     6901 tccgggctgc gacaatcaac agaggaggag aagagtgaca gcagagctgc gtaatctccc
     6961 gcatattgcc agcatggctt ttaatgagcc gctgatgctt gaacccgcct atgcgcgggt
     7021 tttcttttgt gcgcttgcag gccagcttgg gatcagccgc ctgacggatg cagtatccgg
     7081 cgacagcctg gctgccggag aggcacccgc gacgctggcg ttatccggtg atgatgacgg
     7141 accacgacag gcccgcagtt atcaggtcat gaacggcatc gccgtgctgc cggtgtccgg
     7201 cacgctggtc agccggacgc gggcgctgca gccgtattcg ggaatgaccg gttacaacgg
     7261 cattatcgcc cgtctgcaac aggctgccag cgatccgatg gtggacggca ttctgctcga
     7321 tatggacacg ccaggcggaa tggtggcggg agcatttgac tgcgctgaca tcatcgcccg
     7381 tgtgcgtgac ataaagccgg tatgggcgct ggccaacgac atgaactgca gtgcaggtca
     7441 actgcttgcc agcgccgcct cccggcgtct ggtcacgcag accgcccgga caggctccat
     7501 cggcatcatg atggctcaca gtaattacgg tgctgccctg gagaaacagg gcgtggaaat
     7561 cacgctgatt tacagcggca gccataaggt ggatggcaac ccctacagcc atctaccgga
     7621 tgatgtccgg gaaacactgc agtcccggat ggatgcaacc cgccggatgt ttgcacagaa
     7681 ggtgtcggca tataccggcc tgtccgtgca ggctgtgctg gataccgagg ctgcagtgta
     7741 cagcggtcag gaggccattg atgccggact ggctgatgaa cttgttaaca gcaccgatgc
     7801 gatcaccgtc atgcgtgatg cactggatgc acgtaaatcc cgtctctcag gagggcgaat
     7861 gaccaaagag actcaatcaa caactgtttc agccactgct ttgcaggctg acgttactga
     7921 cgtggtgcca gcgacggagg gtgaaaacgc cagcgcggcg cagccagacg tgaacgcgca
     7981 gatcaccgct gcggttgcgg cagaaaacag ccgcattatg gggatcctca actgtgagga
     8041 ggctcacgga cgcgaagaac aggcccgcgt gctggcagaa acccccggta tgaccgtgga
     8101 aacggcccgc cgcattctgg ccgcagcacc acagagtgca caggcgcgca gtgacactgc
     8161 gctggatcgt ctgatgcagg gggcaccggc accgctggct gcaggtaacc cggcatctga
     8221 tgccgttaac gatttgctga acacaccagt gtaagggatg tttatgacga gcaaagaaac
     8281 ctttacccat taccagccgc tgggcaacag tgacccggct cataccgcaa ccgcgcccgg
     8341 cggattgagt gcgaaagcgc ctgcaatgac cccgctgatg ctggacacct ccacccgtaa
     8401 gctggttgcg tgggatggca ccaccgacgg tgctgccgtt ggcattctgg cggttgctgc
     8461 tgaccagacc agcaccacgc tgacgttcta caagtccggc acgttccgtt atgaggatgt
     8521 gctctggccg gaggctgcca gcgacgagac gaaaaaacgg accgcgtttg ccggaacggc
     8581 aatcagcatc gtttaacctt acccttcatc actaaaggcc gcctgtgtgg ctttttttac
     8641 gggatttttt atgtcgatgt acacaaccgc ccagctgctg gcggcaaatg agcagaaatt
     8701 taagtttgat ccgctgtttc tgcgtctctt tttccgtgag agctatccct tcaccacgga
     8761 gaaagtctat ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg tttcgccgat
     8821 tgtttccggt gaggttatcc gttcccgtgg cggctccacc tctgaattta cgccgggata
     8881 tgtcaagccg aagcatgagg tcaatccgca gatgaccctg cgtcgcctgc cggatgaaga
     8941 tccgcagaat ctggcggacc cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg
     9001 tgacgaagag ctggccattg ctcaggtcga agagatgcag gccgtttctg ccgtgctcaa
     9061 gggcaaatac accatgaccg gtgaagcctt cgatccggtt gaggtggata tgggccgcag
     9121 tgtggcgaac aacatcacgc agtccggcgg cacggagtgg agcaagcgtg acaagtccac
     9181 gtatgacccg accgacgata tcgaagccta cgcgctgaac gccagcggcg tggtgaatat
     9241 catcgtgttt gacccgaaag gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa
     9301 gctggatacc cgtcgcggct ctaattccga gctggagaca gcggtaaaag acctgggcaa
     9361 agcggtgtcc tataagggga tgtatggcga tgtggccatc gtcgtgtatt ccggacagta
     9421 cgtggaaaac ggcgtcaaaa agaacttcct gccggacaac acgatggtgc tggggaacac
     9481 tcatgcacgc ggtctgcgca cctatggctg tattcaggat gcggatgcat tgagtgaggg
     9541 tattaatgcg tctccccgtt atccgaaaaa ctggaagaca tccggcgatc cggcgcgaga
     9601 gttcaccatg attcagtcag caccgctgat gctgctggct gatcctgatg agttcgtgtc
     9661 cgttcaactg gcgtaatcat ggcccttcgg ggccattgtc tctctgtgga ggagtccatg
     9721 acgaaagatg aactgattgc ccgtctccgg tcgctgggtg agcaactgaa ccgtgatgtc
     9781 agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg cagagctgga agaggagctt
     9841 gatgacacgg atgacgcagc cggtcaggac acgtctgtca gcccggaaaa tgcgctgacc
     9901 ggacatgaaa atgaggtggt atcagcacag acggataccg tgactgatac ggctgctctg
     9961 gtcacggttg tggcactggt gacgctgcat accgatgcac ttcacgccac gcgggatgaa
    10021 cctgtggcat ttgtgctgcc gggaacggcg ttccgtgtct ctgccggtgt ggcagccgaa
    10081 atgacagaac gtggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
    10141 accagttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
    10201 cagccaccat gacatccggt gagctgtccg gcgcagtaat acgtggtgtt tttgatgacc
    10261 ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt
    10321 ttgtccggac tgatgatgtg cggcaactgc ggcgtggaga cacgctgacc atcggtgagg
    10381 aaaacttctg gatagaccgg gtttcgccgg atgatggtgg aagctgtcat ctctggcttg
    10441 ggcggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
    10501 aggtcttgag caggccgttg aaaacctcag ccgtatcagc agaacggcgg tgcccggtgc
    10561 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtcgg cgtcacaggt
    10621 tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
    10681 tacggtcaaa aatccgcagg ccagaatcaa ggttaaccgg ggggatttgc ccgtaataaa
    10741 gctgggtaac gcgcggattg tcctgtcccg acgcaggcgt cgtaaaaagg ggcagcgttc
    10801 agccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt
    10861 tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag cgtgtggccg ggaaaaaccg
    10921 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
    10981 aaatattgag cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca
    11041 tcaactgagg atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
    11101 gcactggaga agcatgacac cggggcgacg ttttttgatg gtcgccccgc tgtttttgat
    11161 gaggcggatt ttccggcagt tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
    11221 ctggacagcg atacctggca ggcggagctg catatcgaag ttttcctgcc tgctcaggtg
    11281 ccggattcag agctggatgc gtggatggag tcccggattt atccggtgat gagtgatatc
    11341 ccggcactgt cagatttgat caccagtatg gtggccagcg gctatgacta ccggcgcgac
    11401 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattaccta tgaaatgtga
    11461 ggacgctatg cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccacactgtg
    11521 ggtttataag gggaacggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
    11581 tctggctaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
    11641 ctatctcgat gatgaagatg cggactggac tgcgaccggg caggggcaga aatctgccgg
    11701 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
    11761 gtggtttaat gaaggggata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
    11821 tgtgttccgc ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aagtgatcac
    11881 ccgtacggtg aaagtcacca acgtgggacg tccgtcgatg gcagaagatc gcagcacggt
    11941 aacagcggca accggcatga ccgtgacgcc tgccagcact tcggtggtga aagggcagag
    12001 caccacgctg accgtggcct tccagccgga gggcgtaacc gacaagagct ttcgtgcggt
    12061 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaacgg
    12121 cgttgctgca ggcaaggtca acattccggt tgtatccggt aatggtgagt ttgctgcggt
    12181 tgcagaaatt accgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga
    12241 atcatttgaa cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgcat
    12301 tgagcatctc gccctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa
    12361 gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca
    12421 taaccatccg cagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca
    12481 ggaagtgctt accacctggc ccacggaggc aatttctcat gctgaaaacg tggtgtaccg
    12541 gctgtctggt atgtatgagt ttgtggtgaa taatgcccct gaacagacag aggacgccgg
    12601 gcccgcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
    12661 ctggcgcgtg agatggggcg acccgactgg cgtgccatgc ttgccgggat gtcatccacg
    12721 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
    12781 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggatatg
    12841 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
    12901 gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc gctttggccc ggacgggaat
    12961 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    13021 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    13081 cgttgattta agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    13141 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
    13201 ccgacaggcg ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat
    13261 gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag cttgcaggcg ggcaaagtcc
    13321 gtggctgatc ctgctgcaac agggtggtca ggttaaggac tccttcggcg ggatgatccc
    13381 catgttcagg gggcttgccg gtgcgatcac cctgccgatg gtgggggcca cctcgctggc
    13441 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    13501 caacaaaacg ctggtccttt ccggcaatca ggcgggactg acggcagatc gtatgctggt
    13561 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    13621 cgcactggtc aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    13681 ggcgcgtttc tcctctgtat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    13741 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    13801 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    13861 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    13921 gaacatgggc acgctggaga cctgggcaga caggactgcg cgggcattca aatccatgtg
    13981 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    14041 gactgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
    14101 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
    14161 cgcccgaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
    14221 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    14281 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    14341 gaaaatcctg caggcggatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    14401 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
    14461 cagtgctcat gctgacctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    14521 cggagcgaat gagaaaatca gccagcagcg ccgggatttg tggaaggcgg agagtcagtt
    14581 cgcggtactg gaggaggcgg cgcaacgtcg ccagctgtct gcacaggaga aatctctgct
    14641 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
    14701 cacctatcag gagcgcctga acgcgctggc gcagcaggcg gataaattcg cacagcagca
    14761 acgggcaaaa cgggccgcca ttgatgcgaa aagccggggg ctgactgacc ggcaggcaga
    14821 acgggaagcc acggaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
    14881 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
    14941 gatgacaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    15001 gcaggtaaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcagcgat
    15061 gctgaccggc agtgagcaga actggcgcag cttcacccgt tccgtgctgt ccatgatgac
    15121 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    15181 cggggctgtt ggtggcggcg catccgcgtc aggcggtaca gccattcagg ccgctgcggc
    15241 gaaattccat tttgcaaccg gaggattcac aggaaccggc ggcaaatatg agccagcggg
    15301 gattgttcac cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtggg
    15361 gaatctctac cggctgatgc gcggctatgc caccggtggt tatgtcggta caccgggcag
    15421 tctggctgac agccggtcgc aggcgtccgg gacgtttgag cagaataacc atgtggtgat
    15481 taacaacgac ggcacgaacg ggcagatagg tccggctgct ctgaaggcgg tgtatgacat
    15541 ggcccgcaag ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc
    15601 cggaggtgga cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    15661 gccccttctg taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg
    15721 ctgaatgcca acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccacg
    15781 gtactggagt cgtttctgga agagcacggt ggctggaaat cctttctgtg gacgccgcct
    15841 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg
    15901 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    15961 aaacattgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    16021 atctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    16081 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg
    16141 aactgaatgg caaaggcacc agtacgcgcc ccacgctgac ggtttctaac ctgtacggta
    16201 tggtcaccgg gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta
    16261 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    16321 cggagcagga ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtga
    16381 gtgcctcctt tgtactgtcc acgccgacgg aaacggatgg cgctgttttt ccgggacgta
    16441 tcatgctggc caacacctgc acctggacct atcgcggtga cgagtgcggt tatagcggtc
    16501 cggctgtcgc ggatgaatat gaccagccga cgtccgatat cacgaaagat aaatgcagca
    16561 aatgcctgag
//
LOCUS       DAZGCQ010000105        16273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000105 DAZGCQ010000000
VERSION     DAZGCQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.105"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..535
                     /gene="ddpX"
                     /locus_tag="ACHK07_003920"
     CDS             <1..535
                     /gene="ddpX"
                     /locus_tag="ACHK07_003920"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013357056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO8104194.1"
                     /translation="GFYAKSETFGGNGRRTPIYQQARCLLHKDAITALAKSISIAQLS
                     GLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGTAIDLTLRDEHGNILDM
                     GAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGISSEWWHFELPQAASYPL
                     LADQFTCFISPGTQHVS"
     gene            549..2099
                     /locus_tag="ACHK07_003921"
     CDS             549..2099
                     /locus_tag="ACHK07_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8104195.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASGWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRNYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            2101..3123
                     /locus_tag="ACHK07_003922"
     CDS             2101..3123
                     /locus_tag="ACHK07_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8104196.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            3120..4016
                     /gene="ddpC"
                     /locus_tag="ACHK07_003923"
     CDS             3120..4016
                     /gene="ddpC"
                     /locus_tag="ACHK07_003923"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO8104197.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCVFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            4013..4999
                     /locus_tag="ACHK07_003924"
     CDS             4013..4999
                     /locus_tag="ACHK07_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8104198.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            4992..5918
                     /locus_tag="ACHK07_003925"
     CDS             4992..5918
                     /locus_tag="ACHK07_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO8104199.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWTLRKTELPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIRENGRELRCWRAL"
     gene            complement(5974..6405)
                     /gene="osmC"
                     /locus_tag="ACHK07_003926"
     CDS             complement(5974..6405)
                     /gene="osmC"
                     /locus_tag="ACHK07_003926"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO8104200.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            6751..6966
                     /gene="bdm"
                     /locus_tag="ACHK07_003927"
     CDS             6751..6966
                     /gene="bdm"
                     /locus_tag="ACHK07_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO8104201.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7068..7205
                     /gene="sra"
                     /locus_tag="ACHK07_003928"
     CDS             7068..7205
                     /gene="sra"
                     /locus_tag="ACHK07_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO8104202.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            7362..9059
                     /gene="maeA"
                     /locus_tag="ACHK07_003929"
     CDS             7362..9059
                     /gene="maeA"
                     /locus_tag="ACHK07_003929"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8104203.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            9193..10203
                     /gene="adhP"
                     /locus_tag="ACHK07_003930"
     CDS             9193..10203
                     /gene="adhP"
                     /locus_tag="ACHK07_003930"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO8104204.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDV
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            10350..10634
                     /locus_tag="ACHK07_003931"
     CDS             10350..10634
                     /locus_tag="ACHK07_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8104205.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(10862..11515)
                     /gene="fdnI"
                     /locus_tag="ACHK07_003932"
     CDS             complement(10862..11515)
                     /gene="fdnI"
                     /locus_tag="ACHK07_003932"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO8104206.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(11508..12392)
                     /gene="fdnH"
                     /locus_tag="ACHK07_003933"
     CDS             complement(11508..12392)
                     /gene="fdnH"
                     /locus_tag="ACHK07_003933"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO8104207.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(12405..15452)
                     /gene="fdnG"
                     /locus_tag="ACHK07_003934"
     CDS             complement(12405..15452)
                     /gene="fdnG"
                     /locus_tag="ACHK07_003934"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(14865..14867),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8104208.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            15684..>16273
                     /gene="yddG"
                     /locus_tag="ACHK07_003935"
     CDS             15684..>16273
                     /gene="yddG"
                     /locus_tag="ACHK07_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO8104209.1"
                     /translation="MTRQKATLIGLIAIILWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASL"
BASE COUNT         3876 a         4238 c         4214 g         3945 t
ORIGIN      
        1 tggtttttat gctaagagtg agacttttgg aggaaatggg cgcagaaccc ctatttatca
       61 gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa gtatcagtat
      121 cgcccagctc tcaggcttac aactggtgat ttacgatgcg taccgcccac aacaagcaca
      181 ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga cggtcggttc
      241 taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg ggaacatcct
      301 cgatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc acccttccgt
      361 cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg gtggcggttt
      421 tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga gttacccact
      481 gctcgctgat caattcacct gttttatatc ccctggcaca cagcacgtca gttaatcagg
      541 aaactgtcat gaaaagatcg atatcgtttc gtcctacatt gctcgcgctc gtccttgcca
      601 caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt ggtaaggccg
      661 ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg acggtgacct
      721 acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc tcaaccgacg
      781 ttgaaggcga tctggcgagt ggctggaaag cgtctgacga tcaaaaagag tggacgttca
      841 ccctgaagga caatgctaaa tttgccgatg gcacacccgt cactgccgaa gcagtaaaac
      901 tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt cccaaagatt
      961 taaagattga tgctcccgac gaacatacgg tgaagtttac ccttagccag ccatttgcac
     1021 cgttcctcta cacgctggcg aatgacggtg catccattat caatccggcg gtgttaaagg
     1081 aacatgcggc ggatgatgcc cgcggcttcc tcgcgcaaaa taccgccggt tccggaccat
     1141 ttatgctgaa aagctggcaa aaaggtcagc aattagttct ggtgccaaat ccgcattacc
     1201 ctggcaataa accgaacttt aagcgagtat cggtaaaaat tattggtgaa agtgcctccc
     1261 gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg gtggatcaac
     1321 tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca ctgcgcgtca
     1381 cctatctgta tctgaataac agcaaagcgc cacttaatca ggcggatctg cggcgggcca
     1441 tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt aacggaaaac
     1501 agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca atgcaataca
     1561 accatgacga aacgaaagcc aaagctgaat gggataaagt gacgaacaaa cccaccagcc
     1621 tgacgtttct ctattctgat aatgatccga actgggagcc tattgctctg gcgacacaat
     1681 ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac gccaccatgc
     1741 gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt ccggattttg
     1801 ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt ctgccgggta
     1861 accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg ctcgcgacca
     1921 ccgaccagac gcagcgtacc cggaactacc agcaggcaca gaaaatcgtc attgatgacg
     1981 ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa gaggtgaaag
     2041 gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg agtaaataac
     2101 gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt ggtggcgggc
     2161 gtctgcgtga ttacgtttat tatctcgcat ctgatccccg gcgatccggc gcggttactg
     2221 gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact gggactggac
     2281 cagccactgt atgtacagtt ttaccgctac gtcagcgacc tgtttcatgg tgacctggga
     2341 acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt cccggcgacg
     2401 ctggagctgg ctttttgtgc cctgctgctg gcactcttga ttggcatccc gctgggcata
     2461 ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat ggccattacc
     2521 ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt ttatggtcat
     2581 ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc aacgcacgtt
     2641 accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtatt cttcaatgcg
     2701 ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg aattgtcgct
     2761 cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg taccgcccgg
     2821 gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa tgcgttgatc
     2881 ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc agtgctcacc
     2941 gaaaccgttt ttgcctggcc cggaatgggg gcatgggtag tgacatcaat acaggcgctc
     3001 gacttccctg cggtgatggg atttgccgtc gtggtttcgt ttgcttatgt gctggtcaac
     3061 ctggtagtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg aggtgatgaa
     3121 tgatgctaag cgaggaaacg tccgccgtac gcccagcaaa acaaacgcga tttaacggtg
     3181 caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg
     3241 tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat cccaacgcca
     3301 ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc accgatgaag
     3361 tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat
     3421 tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta tccggtgtgc
     3481 ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg tcgattcctt
     3541 cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt aacgccatgc
     3601 tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg caagcattag
     3661 ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg cgttggcatc
     3721 tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag gcatcgctgg
     3781 atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg ggtgctcaac
     3841 aaccgagtgc tgaatggggc gcgatggtag caaatggtcg caactatgta ctcgatcagt
     3901 ggtggtattg cgtatttccg ggggcagcga ttttgcttac cgccgtcggg tttaatctct
     3961 ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca
     4021 acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg gtgatgttca
     4081 cgcgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc tggtgggaga
     4141 atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac cgacgggcag
     4201 ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta acgctcggga
     4261 aaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg aaccgatgac
     4321 cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc gccatcatca
     4381 accaataagt cgtcgggaag ccagagctaa agcgattgcc ctgctggaag agatgcaaat
     4441 cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca
     4501 gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg ccgacgaacc
     4561 gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta aacataaagc
     4621 ccgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg tgtcgcaact
     4681 gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg ggacggcaga
     4741 cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg caccagaaca
     4801 tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc tcacccattt
     4861 gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt gtgaaaacgt
     4921 cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt atccgcagca
     4981 ggaggtcata agtgtctgac acgttattaa cgttacgcga cgtccatatc aatttcccgg
     5041 cccgtaaaaa ctggcttggt aaaactacgg aacatgttca tgccattaat ggtattgatt
     5101 tacagatccg ccgtggtgaa accttaggga tcgtcggcga gtcaggctgc ggcaaaagca
     5161 ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac atccgttctg
     5221 gctcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctc aatccgcgct
     5281 taccggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt agtgaacaac
     5341 agcggcgagc gttggcagag gagctggctg tgcaggtggg tattcgtccg gagtatctcg
     5401 accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt gccagagcac
     5461 tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg gatatctccg
     5521 tgcaggcgca gatcctcaat ttactggtaa cgctacaggt aaatcgcggg ctgacctatg
     5581 tgctgatctc acacaatgtc tcggtgattc gtcatatgag cgatcgggta gcggtgatgt
     5641 atctcgggca gattgtagaa ctgggcgacg cgcaacaggt actgacggct ccggcacatc
     5701 cgtacacccg attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat
     5761 ggacattacg taaaacggaa ctgccgggaa accgcacgtt gccgcaaggc tgttttttct
     5821 acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta gcaataaggg
     5881 agaacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc
     5941 attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagtgt
     6001 aatttccgct ttcagtacct gagagaccgg gcatcctgct tttgctttct ggatgatgcc
     6061 gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt
     6121 aatcgcaaaa ccggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt
     6181 tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg
     6241 cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa
     6301 tccatacggc tgttggttca gcacgccact ctcggtggat actgttccct tcccgcgttt
     6361 gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg
     6421 ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc
     6481 gaataaaatc cggcagaaaa tattacttct gcacttaaat taaatttcca caatagataa
     6541 ccgggaatat atagctcaag agatattgca tcaatatcat tcaactcacc acaataataa
     6601 tacctatcaa tatcatgggg ttaaaatata aaaatcatca accaggacta atcttaacaa
     6661 cgaagcggca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
     6721 actataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca
     6781 acagcagaac ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcacggtgtt
     6841 gtcactgaag atgacattct catggagctg accaaatggg tggaagcttc tgataacgac
     6901 atcctcagtg atatctacca gcaaacgatc aattatgtgg tcagtggcca gcaccctacg
     6961 ctttaagatg ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc
     7021 tacggcgatg ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc
     7081 gtcaggcacg tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta
     7141 ccgaaggtga caaatccagc gtggtaaata acccaaccgg cagaaaacgc cccgctgaaa
     7201 agtaattcat aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt
     7261 ttttgttttt atctgcttta tacttggggc cgacgccctg gcggtaaagc aaagacgata
     7321 aaagcgtgcc agggatggat attcaaaaaa gagtgagtga catggaacca aaaacaaaaa
     7381 aacagcgttc gctttatatc ccttacgctg gccctgtact gctggaattt ccgttgttga
     7441 ataaaggcag tgccttcagc atggaagaac gccgtaactt caacctgctg gggttactgc
     7501 cggaagtggt cgaaaccatc gaagaacaag cggaacgagc atggatccag tatcagggat
     7561 tcaaaaccga aatcgacaaa cacatctacc tgcgtaacat ccaggacacc aacgaaaccc
     7621 tcttctaccg tctggtaaac aatcatcttg atgagatgat gcctgttatt tataccccaa
     7681 ccgtcggcgc agcctgtgag cgtttttctg agatctaccg ccgttcacgc ggcgtgttta
     7741 tctcttacca gaaccgccac aatatggacg atattctgca aaacgtacca aaccataata
     7801 tcaaagtgat tgtggtgact gacggtgaac gtattctggg gcttggtgac cagggcatcg
     7861 gcgggatggg cattccgatc ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc
     7921 cggcgtatac ccttccggtg gtgctggatg tcggcaccaa caaccaacag ctgcttaacg
     7981 atccgctgta tatgggctgg cgtaatccgc gtatcactga cgacgaatac tatgaattcg
     8041 ttgatgaatt tatccaggct gtgaaacaac gctggccgga cgtgctgttg cagtttgaag
     8101 acttcgcaca aaaaaatgcg atgccgttac ttaaccgcta tcgcaatgaa atttgttcct
     8161 ttaacgatga cattcagggc actgcggcgg taacagtcgg caccctgatc gcagcaagcc
     8221 gcgcggcagg tggtcagtta agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg
     8281 gatgcggcat tgccgaaatg atcatcgccc agacccagcg cgaaggatta agcgaggaag
     8341 cggcgcggca gaaagtcttt atggtcgatc gctttggctt gctgaccgac aagatgccga
     8401 acctgctgcc tttccagacc aaactggtgc agaagcgcga aaacctcagt gactgggata
     8461 ccgacagcga tgtgctgtca ctgctggatg tggtgcgcaa tgtaaaacca gatattctga
     8521 ttggcgtctc aggacagacc gggctgttta cggaagagat catccgtgag atgcataaac
     8581 actgtccgcg tccgatcgtg atgccgctgt ctaacccgac gtcacgcgtg gaagccacac
     8641 cgcaggacat tatcgcctgg actgaaggta acgcgctggt cgccacgggc agtccgttta
     8701 atccagtggt atggaaagat aaaatctacc ctatcgccca gtgtaacaac gcctttattt
     8761 tcccgggcat cgggctgggt gttattgctt ccggcgcgtc acgtatcacc gatgagatgc
     8821 tgatgtcggc aagtgaaacg cttgctcagt attcgccgct ggtcctgaac ggcgaaggtc
     8881 tggtactacc ggaactgaaa gatattcaga aagtctcccg cgcaattgcg tttgcggttg
     8941 gcaaaatggc gcagcagcaa ggcgtggcgg tgaaaacgtc tgccgaagct ctgcaacagg
     9001 ccattgatga taatttctgg caagccgaat accgcgacta ccgccgtacc tccatctaag
     9061 cctgcgcccg gtagtgaagg ctaccgggca attttcctct ccctttttca gatctcatcc
     9121 atactgggta gtggcgaata aatctcattt gcctcacctg ctatgcagaa catcatccga
     9181 aaaggaggaa ctatgaaggc tgcagttgtt acgaaggatc atcatgttga cgttacggat
     9241 aaaacactgc gctcactgaa acatggcgaa gccctgctga aaatggagtg ttgtggtgta
     9301 tgtcataccg atcttcatgt taagaatggc gattttggtg acaaaaccgg cgtaattctg
     9361 ggccatgaag gcatcggtgt ggtggcagaa gtgggtccag gtgtcacctc attaaaacca
     9421 ggcgatcgtg ccagcgtggc gtggttctac gaaggatgcg gtcattgcga atactgtaac
     9481 agtggtaacg aaacgctctg ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg
     9541 atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa aagtgccaga tggtctggac
     9601 tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca cctacaaagc cgttaaactg
     9661 tcaaaaattc gtcccggtca gtggattgct atctacggtc ttggcggtct gggtaacctc
     9721 gccctgcaat acgcgaagaa tgtctttaac gcgaaagtga tcgccattga tgtcaatgat
     9781 gagcagttaa aactggcaac cgaaatgggt gcagatttag cgattaactc acgcaccgaa
     9841 gacgtcgcca aaattgtgca ggagaaaacc ggtggcgctc acgctgcggt ggtaacagca
     9901 gttgctaaag ctgcgtttaa ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg
     9961 gctgtcggtc tgccgccgga gtctatgagc ctggatatcc cacgtcttgt gctggatggt
    10021 attgaagtgg tcggttcgct ggtcggcacg cgccaggatt taactgaagc cttccagttt
    10081 gccgccgaag gtaaagtggt gccgaaagtc gccctgcgtc cgttagcgga catcaacacc
    10141 atctttaccg agatggaaga aggcaaaatc cgtggccgca tggtgattga tttccgtcac
    10201 taagaggcct ttgctgcgac tgccagtcct ggtgtcgcag catcgcacac tctccaacat
    10261 gaaatggcta aatggattat tgttgcgtag gaaggaacaa taatgatagt gttcccagtg
    10321 aaggaacact actgagagta aagacactga tgaaaatggc caatcatccc cgcccggggg
    10381 acattattca ggaatcactg gatgaactta atgtcagcct gcgcgagttt gccagagcaa
    10441 tggaaattgc gccctcaacg gcaagccgat tgctgaccgg aaaagcagct ttgacgccag
    10501 aaatggcaat taaactttcc gtggtgattg gcagttcgcc gcaaatgtgg ctgaatctgc
    10561 aaaacgcctg gagtctggca gaggctgaaa aaacggtgga tgtgtcgcga ttacgccgtc
    10621 tggttacgca ataaaaaatg gcaccttata aagcgccatt tgaaactgat gacaaatgca
    10681 acattgcctg atgcgctacg cttatcaggc ctacacgttc actgcaatct attgtatttg
    10741 aagggttttc atgccggata aggcattcac gacgcatccg ggatgaacaa agcccacgtt
    10801 gtctacaata tgaaaatggc gccttttaca gcgccatttt cagcttaaag ttcaatagag
    10861 attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca cgataccagc
    10921 gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc cctttaatcg
    10981 atcctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc aggatgatac
    11041 ccgcagccgc gtggatcagc aggctgtagc gaacaacctg catcgggaag tactgcgcaa
    11101 agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg ctcatgatcg
    11161 accagaacat catcttttgc ccggcgttgt acttaccgac atccgccact ttgtgctcat
    11221 tgccttttaa tacttcgaca atgttcaaca gccacggaat atctttctta tccgggatgt
    11281 tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca atgccgaaga
    11341 acgggtgcaa aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg
    11401 tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc accacggtcc
    11461 agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta ctcatgatga
    11521 tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtaatg gaaaatcaac
    11581 cctgcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc tttccacagg
    11641 cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc cggctgatcg
    11701 gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca
    11761 gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct gttccgccag ctccagcatc
    11821 tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca agccggttcc
    11881 tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt atcctctttg
    11941 ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc gatgcagttt
    12001 tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccagc agacgggcac
    12061 gccttcaggc agccgggatc ttcacagtgc atacagccgt ctttacggat cagccactcc
    12121 agcttgccgt tctgttcggt ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga
    12181 tcggcggggt tatcataaac cccgacgcag tgccccactt catcacggat gtcgttccac
    12241 tccgaacacg ccacctgaca ggctttacag ccgatacagg tggaaacgtc gataagtttc
    12301 gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt agttgcggac
    12361 cttttgataa tgtcctgcgt ttccatagcc atctgttcgc ccccttacgc cttctcgatg
    12421 ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac attcggcgtc
    12481 agagtgttag cgatataacc tttacgcgcg acaccctcaa agccccagtg gattggaata
    12541 cccaccgttt caacctgctg accgtttaca ttcagcggtt tcagacggcg cgttaccaca
    12601 gccaccgcgc ggataaagcc acgcttgctt gagacagtga cacgatcgcc attattaatg
    12661 cctttcgccg ccgccagcgt ttcgctgatt tccacaaact gttccggctg agcaattgcg
    12721 ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc
    12781 acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata cagacgaacg
    12841 accgggttag acaccacgtt cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc
    12901 tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag gcgtcccatc
    12961 ccttccggct gcataataaa cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca
    13021 ggaatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg tttcggatcc
    13081 cacggtttac cgttgatatc cgccgaagca cggttgtaca gcacgcgacg gttgagcggc
    13141 cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt atcgcggtta
    13201 gccatctggt tgccctgctc tgtccagcta ccggtgtaga tccagcaaga agatgcggtt
    13261 gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gacctttctt cgcaatcagc
    13321 acgccattag catcatagag atcttccagc gcatagccgt tgttctcttt tgccacttcg
    13381 tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag cggttctacg
    13441 cctttaccac cttcggcctg gtacagctcg cgcagatgat ggtaaatacc cgccagaatt
    13501 tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag
    13561 cgaccggagt tagcgataga accatcttct tcagcaaagc aggtcgaagg cagacggaat
    13621 acttcagtct gaatagacgc cggatcgaca tcgttcgact cgccgtggtt ctgccagaag
    13681 gtagaggttt cagtcaccag cggatcgata accaccatgt acttcagctt gctcaggcag
    13741 ctcaccactt tgtttttgtc cgggaaggac gcaaccgggt taaagccctg gcagaaataa
    13801 ccggtgactt tgccttcgtc catcatgttg aaatacttga tgacgtcgta ggtctggtcc
    13861 cacttcggca gccagtcata gccccagttg ttctctttct gcgcggcatc gccatagaaa
    13921 gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac ctgatcagcc
    13981 agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg tttttctgac
    14041 ggcagcgtca gataacctgg caggctggta gagagcagac ctaagtcagt cagaccctga
    14101 atgttggagt gaccacgcaa tgcattcacg ccgccaccgg ccatacccat gttaccgagc
    14161 agcaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag
    14221 cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc cagcacttca
    14281 cacactttca ggaagtcagc ttttggcgta ccacagatgt tttcaacaac gtccggcgtg
    14341 tagcgggaaa cgtgcgcttt cagcaggttc cacacacagc gcggatgagt cagtgtttca
    14401 tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc aggacgattt atcgtattgc
    14461 cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc
    14521 agcaagctgg cgttggtgta atgcttaacg tattcggcgt tgattttgtt gttttcgatc
    14581 aggtagcgca aaacgccaga caggaacgta atgtccgtac cggaacgaat aggtgcgtaa
    14641 atatccgcta cagaagcggt acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg
    14701 ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta
    14761 ccgcccatta ccattacgac gttagcgttt ttgatatcca cccagtggtt ggtcatcgca
    14821 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac gcgcgcctgg
    14881 ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat cccggtttcg
    14941 ttgctggcac cggaagcaca cagcataccg gtagaaagcc aacggtttac cgttacgccc
    15001 tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt cgcaatacgg
    15061 gagaatgctt cttcccagct aatgcgctgc catttgtcag aacctggcgc acgatattcc
    15121 gggtagcgca gacggttttc gctgttgacg taatccagca aaccggcccc tttcgggcac
    15181 agcgcaccac ggcttaccgg atgatccgga tcaccttcaa tgtgataaat cgcttctctg
    15241 gcgtttttcg cgccatcacc caggctatac atcaatagcc cgcaacctac ggaacagtat
    15301 gtgcaggtgt tacggatctc tttagcgcgt aataatttgt agtttcgcgc ctgagccagt
    15361 gcttgcttcg gggcaaagcc caatgccgct actgttgttc cagccatacc gcccgcgcag
    15421 attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca ggggtattac
    15481 tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa aaagaacacg
    15541 tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac
    15601 gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta atttttaacg
    15661 gcaaaagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata gggctgatag
    15721 cgatcatcct gtggagcacg atggtaggat tgattcgcgg tgtcagtgag gggctcggcc
    15781 cggtcggcgg cgcagctgct atctattcat taagcgggct gctgttaatc ttcacggttg
    15841 gatttccgcg tattcggcaa atcccgaaag gctatttact cgccgggagt ctgttattcg
    15901 tcagctatga aatctgtctg gcgctttcct tagggtatgc ggcgacccgt catcaggcga
    15961 ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt gccattctgt
    16021 ttaatggtca gaaaaccaac tggctgattg tacctggatt attattagcc ctcgtcggcg
    16081 tctgctgggt gttaggcggt gacaatgggt tacattatga tgaaatcatc aataatatca
    16141 ccaccagccc attgagttat ttcctggcgt tcattggtgc gtttatctgg gcagcctact
    16201 gcacagtaac gaataaatac gcacgcggat ttaatggaat taccgttttt gtcctgctaa
    16261 cgggagcaag tct
//
LOCUS       DAZGCQ010000106        15729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000106 DAZGCQ010000000
VERSION     DAZGCQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.106"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            343..1749
                     /gene="chiP"
                     /locus_tag="ACHK07_003936"
     CDS             343..1749
                     /gene="chiP"
                     /locus_tag="ACHK07_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO8104210.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            1799..2125
                     /gene="chiQ"
                     /locus_tag="ACHK07_003937"
     CDS             1799..2125
                     /gene="chiQ"
                     /locus_tag="ACHK07_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO8104211.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(2209..2655)
                     /gene="fur"
                     /locus_tag="ACHK07_003938"
     CDS             complement(2209..2655)
                     /gene="fur"
                     /locus_tag="ACHK07_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO8104212.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(2648..2734)
                     /gene="uof"
                     /locus_tag="ACHK07_003939"
     CDS             complement(2648..2734)
                     /gene="uof"
                     /locus_tag="ACHK07_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO8104213.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            complement(2944..3474)
                     /gene="fldA"
                     /locus_tag="ACHK07_003940"
     CDS             complement(2944..3474)
                     /gene="fldA"
                     /locus_tag="ACHK07_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO8104214.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            complement(3614..3907)
                     /gene="ybfE"
                     /locus_tag="ACHK07_003941"
     CDS             complement(3614..3907)
                     /gene="ybfE"
                     /locus_tag="ACHK07_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO8104215.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            complement(4047..4811)
                     /gene="ybfF"
                     /locus_tag="ACHK07_003942"
     CDS             complement(4047..4811)
                     /gene="ybfF"
                     /locus_tag="ACHK07_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8104216.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4996..5541
                     /gene="seqA"
                     /locus_tag="ACHK07_003943"
     CDS             4996..5541
                     /gene="seqA"
                     /locus_tag="ACHK07_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO8104217.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5567..7207
                     /gene="pgm"
                     /locus_tag="ACHK07_003944"
     CDS             5567..7207
                     /gene="pgm"
                     /locus_tag="ACHK07_003944"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO8104218.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7382..8059)
                     /gene="kdpE"
                     /locus_tag="ACHK07_003945"
     CDS             complement(7382..8059)
                     /gene="kdpE"
                     /locus_tag="ACHK07_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO8104219.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8056..10740)
                     /gene="kdpD"
                     /locus_tag="ACHK07_003946"
     CDS             complement(8056..10740)
                     /gene="kdpD"
                     /locus_tag="ACHK07_003946"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO8104220.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLLVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            complement(10733..11305)
                     /gene="kdpC"
                     /locus_tag="ACHK07_003947"
     CDS             complement(10733..11305)
                     /gene="kdpC"
                     /locus_tag="ACHK07_003947"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO8104221.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(11314..13362)
                     /gene="kdpB"
                     /locus_tag="ACHK07_003948"
     CDS             complement(11314..13362)
                     /gene="kdpB"
                     /locus_tag="ACHK07_003948"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO8104222.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTCILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            complement(13385..15058)
                     /gene="kdpA"
                     /locus_tag="ACHK07_003949"
     CDS             complement(13385..15058)
                     /gene="kdpA"
                     /locus_tag="ACHK07_003949"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO8104223.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPFGSGLARLINDIPLPGTAGVER
                     VLFSALGVSDREMNWKQYLSAILCLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFSLIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFQPYQAVTTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            complement(15058..15147)
                     /gene="kdpF"
                     /locus_tag="ACHK07_003950"
     CDS             complement(15058..15147)
                     /gene="kdpF"
                     /locus_tag="ACHK07_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO8104224.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            15460..15666
                     /gene="ybfA"
                     /locus_tag="ACHK07_003951"
     CDS             15460..15666
                     /gene="ybfA"
                     /locus_tag="ACHK07_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO8104225.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT         3875 a         4256 c         4053 g         3545 t
ORIGIN      
        1 cacgaattaa ttccgcttcg caatttatcc ataaaataaa tttaaaataa caaaacataa
       61 ttaaataaaa tgtaaccgct ttcatcttgc tggaaattca cgcttttatt cttctgcaag
      121 cctttcaacc gcaaacttaa gccttgtaac aaaaatcatc aaaatatgtg cggttgctca
      181 tgttcttaca ttctggttac agaaagagat tgataattcg cgccgcgaaa aatagtctgt
      241 tcctgtagtc agcgagactt ttctcaacgc tactttttta atttttattt tttcgctgtt
      301 cacctttggt gcagcaattt atacgtcaaa gaggattaac ccatgcgtac gtttagtggc
      361 aaacgtagta cgctggcgct ggctatcgcc ggtgttacag caatgtcggg ctttatggca
      421 atgccggagg ctcgcgccga aggattcatc gacgattcaa ccttaaccgg cggtatctat
      481 tactggcagc gtgaacgcga ccgtaaagat gttaccgacg gcgacaaata caaaaccaac
      541 ctttctcact ccacctggaa tgccaacctc gattttcagt ctggttatgc tgctgatatg
      601 ttcggccttg atattgccgc gtttacggcg attgaaatgg cggaaaacgg cgacagctct
      661 cacccgaacg aaatcgcgtt ttcaaaaagt aataaagcct atgacgaaga ctggtccggc
      721 gataaaagcg gtataagcct gtataaagca gcggccaaat ttaaatacgg tccggtttgg
      781 gcgagggcag gttatattca gccaaccggt cagacgctgt tagcgccgca ctggagcttt
      841 atgccaggta cctatcaggg tgcggaagcc ggagcgaatt ttgattacgg cgatgccggt
      901 gcgttgagtt tctcctacat gtggaccaac gaatacaaag caccgtggca tctggaaatg
      961 gatgagtttt atcagaacga taaaactacc aaagttgatt atctgcactc catcggtgcg
     1021 aaatacgact tcaaaaataa cttcgtactg gaagcggcat ttggtcaggc ggaagggtat
     1081 atcgatcaat actttgccaa agccagctac aaatttgata tcgccggtag cccgttaacc
     1141 accagctacc agttctacgg tacgcgcgat aaagttgacg atcgcagcgt caacgatctt
     1201 tatgacggca ccgcctggct gcaagcgttg acctttggtt accgggcggc tgacgtagtg
     1261 gatttgcgcc tcgaaggcac ctgggttaaa gctgacggtc agcagggata cttcctgcaa
     1321 cgtatgactc caacctacgc ttcctcgaac ggtcgcctgg atatctggtg ggataaccgt
     1381 tctgacttca acgccaacgg cgaaaaagcg gtcttcttcg gtgcgatgta tgacctgaaa
     1441 aactggaatc ttccaggctt cgccatcggc gcttcctacg tttacgcatg ggatgctaaa
     1501 cctgcgacct ggcagagcaa tccggatgcg tactacgaca aaaaccggac tattgaagag
     1561 tctgcctaca gcctggatgc ggtctacacc attcaggacg gtcgcgccaa aggcacgatg
     1621 ttcaaactgc acttcaccga atacgacaac cactccgaca tcccaagctg gggcggcggt
     1681 tacggtaaca tcttccagga tgagcgtgac gtgaaattta tggtaatcgc accattcacc
     1741 atcttctgat gcccgacgcg gcaggttttg tgcctgccgc acgtttgagg aatttgctat
     1801 gaaaaaactg attctcatcg ccatgatggc atcggggctg gtagcttgtg cgcaatcaac
     1861 cgcaccacag gaagacagcc gtctgaaaga ggcatacagc gcctgtatca acaccgcaca
     1921 aggttcgccg gaaaaaattg aagcctgcca gagcgtgtta aacgtgctga agaaagagaa
     1981 acaacatcag cagtttgccg accaggaaag tgtccgcgta ctggattatc agcagtgtct
     2041 acgtgcaacg caaaccggaa atgatcaggc ggtgaaagcc gattgtgata aggtctggca
     2101 ggaaattcgc agtaataaca aataagtgag agctgtaact ctcgcttttc ttatttccct
     2161 tgcataaaaa agccaacccg caggttggct tttctcgttc agactggctt atttgccttc
     2221 gtgcgcgtgc tcatcttcgc ggcaatcgcc ttcggcacag tgaccgtaaa gatagagact
     2281 gtggttagtc aggcgaatgc catgttttgc ggcaatttca cgctgacgcg cttcgattga
     2341 atcatcacta aattcgataa ccttgccgca gtcgaggcag atcaggtgat cgtggtgatg
     2401 ttgctgtgtc agttcaaata cggatttacc gccttcaaaa ttgtggcggg tgacgatacc
     2461 agcgtcgtca aactggttca gtacgcgata taccgtagcc agaccaattt cttcacccat
     2521 atcgatcaga cgtttgtata aatcttccgc actgacgtga tggttgtccg gctcctgaag
     2581 aacttccagg atttttaaac gtggaagcgt tactttcagg ccagctttct ttagggcggt
     2641 attgttatca gtcatgcgga atctgtcctg ttactaagcg gttcacttca ttagaagaag
     2701 tgacagaatt tgctcttgag ataatgcgta tcattataga attgccacgc ctaaatgaaa
     2761 accacaagtc cctggcaaat attgttaata aaaacgtggc acagcttaca tttacaacgg
     2821 caaggccact tgaacaccgg gacattgtac aggtacaaca gcaaaagtta caaatttgta
     2881 gcaattattt tgattggcat tatctattaa tacggcgtag acatgagtct acgccgcatc
     2941 acatcaggca ttgagaattt cgtcgagatg caactcttca gaaatctgtt taacccattt
     3001 ttctacacgt tcagcggtca gttccggctg acggtcttcg tcgatagcca gaccgacaaa
     3061 gtggtcgtca tctgccagac cttttgatgc ttcgaaatga tagcccgcag ttggccagtg
     3121 accaacgatg gttgcaccgc gcggttcaat gatgtcgcgg atggtgccca atgcgtcgca
     3181 gaaatattcg gcgtaatctt cctggtcacc acaaccaaac agcgcaacca gtttgccatt
     3241 gaaatcaatc tcttcgagag tcgggaagaa gtcatcccag tcacactgcg cttcgccgta
     3301 ataccaggtt gggatgccca gcagcagaat gtcataagct tccagatctt ctttgctgct
     3361 ttttgcaatg tcatggacat cggcaacgtc tttaccaagc tgtttttgaa tcatttttgc
     3421 gatattttcg gtattaccgg tgtcgctgcc gaaaaagatg ccagtgatag ccatgagtga
     3481 aataacctct tgaaacttat tgaaatgggg gtggaaaatt gcccacggat aaaggcaatc
     3541 atagcagaac aggcagtctt gcggaatcag caaacgagca ggactgcaca ctgtgctaca
     3601 tgaaagtgga aatttaaacg atgccctgac tacgcagcgc cgccagttgc tgcattaaca
     3661 tctcttcgat cagttcgcta cggctcatat tgcgcgattc cgccagttcg ttcagcgcct
     3721 cgacagcttc cgcgttcagc ttcagttcga cacgcttaag gccacgtact ttgtcgcgtt
     3781 ttagttggtt gcgtttatta atacgcagct gttcatcgcg cgaaagcgga ttagttttcg
     3841 gtcgtcccgg tcgacgctcg tgcgcgaaca gatctaatgt cgtacggtcc gtttgttctt
     3901 tggccatgat cttggtgact tcgggggaaa caatcagcca ggcgcctgcc cggatggata
     3961 gcgcgccata atacaacagc gagaggagtc tcgccaacgc ccacgcgcgg aaagcgacgc
     4021 ggacgctggg tttttaatca gttgcgttaa tcattgagat agcgacggat agcgcgtaat
     4081 accgcatctg gtttttcagc atggacccag tgacccgcgc ctgcaatcac atgcgcccgt
     4141 gcctgtggaa attgtgccag taaatcatca cggtactgct cgctaacata cggagaattg
     4201 ccgccaggga taaacagggc agggtgatcc catgccggga ttttctccca acccacaata
     4261 tgcggatact gatcccacaa taccggcacg ttaaagcgcc actccccgtc aacaaaagat
     4321 ttcaacagaa actgaatcac cccttcttca ttaagatgct ggcgcattat tgctgctgct
     4381 tgctggcgag tttgtgcgtc cgattcactg accgcgttga tagccgagaa aatctcatca
     4441 tgacggcgta cgtgatagtc gaccggtgcg atatcgatcg ccaccagttt atcgatgcga
     4501 tcgggggcca gtgcggtaag tgccattacc gctttaccgc ccatggagtg accgataaat
     4561 gttgctttgt cgatctgcag tgcatccagg gtatcaacaa gatcctgcgc catcgtcggg
     4621 taattcatta ccggatctct cggtgaaaga ccgtggttac gcatatcaac ctggatgata
     4681 ttgtgatcgt ttaccagatc gcgagccagt acgccaaggt tgtcgaggct gccaaacaga
     4741 ccatggacaa gaacgatggg agaattattg tgctggtttt gtgcagtttg cgcgcggata
     4801 ttcaatttca tggcaaagtt ctttttttcg cgttgtcggg ttagggtatt atgttgacca
     4861 ttgtgccaca gggctgcaac aaataaggtt tattccgagt ttttctgcaa gccaggcttg
     4921 acgctatccg ctgccgggat ttattcatat actcctggcg acttgtattc agctaagaca
     4981 ctgcactgga ttaagatgaa aacgattgaa gttgatgatg aactctacag ctatattgcc
     5041 agccacacta agcatatcgg cgagagcgca tccgacattt tacggcgtat gttgaaattt
     5101 tccgccgcat cacagcctgc tgctccggtg acgaaagagg ttcgcgttgc gtcacctgct
     5161 atcgtcgaag cgaagccggt caaaacgatt aaagacaagg ttcgcgcaat gcgtgaactt
     5221 ctgctttcgg atgaatacgc agagcaaaag cgagcggtca atcgctttat gctgctgttg
     5281 tctacactat attctcttga cgcccaggcg tttgccgaag caacggaatc gttgcacggt
     5341 cgtacacgcg tttactttgc ggcagatgaa caaacgctgc tgaaaaatgg taatcagacc
     5401 aagccgaaac atgtgccagg cacgccgtat tgggtgatca ccaacaccaa caccggccgt
     5461 aaatgcagca tgatcgaaca catcatgcag tcgatgcaat tcccggcgga attgattgag
     5521 aaggtttgcg gaactatcta aaacgttgca gacaaaggac aaagcaatgg caatccacaa
     5581 tcgtgcaggc caacctgcac aacagagtga tttgattaac gtcgcccaac tgacggcgca
     5641 atattatgta ctgaaaccag aagcagggaa tgcggagcac gcggtgaaat tcggtacttc
     5701 cggtcaccgt ggcagtgcag cgcgccacag ctttaacgag ccgcacattc tggcgatcgc
     5761 tcaggcaatt gctgaagaac gtgcgaaaaa cggcatcact ggcccttgct atgtgggtaa
     5821 agatactcac gccctgtccg aacctgcatt tatttccgta ctggaagtgc tggcagcgaa
     5881 cggcgtagat gttattgtgc aggaaaacaa tggcttcacc ccgacgcctg ccgtttccaa
     5941 tgccatcctg gttcacaata aaaaaggtgg cccgctggca gacggtatcg tgattacacc
     6001 gtcccataac ccgccggaag atggtggtat caagtacaat ccgccaaatg gtggcccggc
     6061 agataccaac gtcactaaag tggtggaaga cagagccaac gcactgatgg ccgatggcct
     6121 gaaaggcgtg aagcgtatct ccctcgacga agcgatggca tccggtcatg tgaaagagca
     6181 ggatctggtg cagccgtttg tggaagggct ggccgatatc gttgatatgg cggcgattca
     6241 gaaagcgggc ctgacgcttg gcgttgatcc gctgggcggt tccggtatcg aatactggaa
     6301 gcgtattggc gagtattaca acctcaacct gactatcgtt aacgatcagg tcgatcaaac
     6361 cttccgcttt atgcaccttg ataaagacgg cgcgatccgt atggactgct cctccgagtg
     6421 tgcgatggcg ggcctgctgg cactgcgtga taagttcgat ctggcgtttg ctaacgaccc
     6481 ggattatgac cgtcacggta tcgtcactcc ggcaggtttg atgaatccga accactacct
     6541 ggcggtggca atcaactacc tgttccagca tcgtccgcag tggggcaaag atgttgccgt
     6601 tggtaaaacg ctggtttctt ctgcgatgat cgaccgtgtg gtcaatgact tgggtcgtaa
     6661 gctggtagaa gtcccggtag gtttcaaatg gtttgttgat ggtctgttcg acggcagctt
     6721 cggctttggc ggcgaagaga gcgcaggggc ttccttcctg cgtttcgacg gcacgccgtg
     6781 gtccaccgac aaagacggca tcatcatgtg tctgctggcg gcggaaatca ccgctgtcac
     6841 cggtaagaac ccgcaggaac actacaacga actggcaaaa cgctttggtg cgccgagcta
     6901 caaccgtttg caggcagctg cgacttccgc acaaaaagcg gcgctgtcta agctgtctcc
     6961 ggaaatggtg agcgccagca ccctggcagg tgacccgatc accgcgcgcc tgactgctgc
     7021 tccgggcaac ggtgcttcta ttggcggtct gaaagtgatg actgacaacg gctggttcgc
     7081 cgcgcgtccg tcaggcacgg aagacgcata taagatctac tgcgaaagct tcctcggtga
     7141 agaacatcgc aagcagattg agaaagaagc ggttgagatt gttagcgaag ttctgaaaaa
     7201 cgcgtaaaca cattaaataa gaaaaagggc ggtcggaaga tcgccctttt ttacgtatga
     7261 caaacacaga attgcctgat gcgctacgct tatcaggcct acgaggatgg tgcaatatat
     7321 tgaatttacg cgattttgta ggccggataa ggcgttcatg ccggatccag catttaaccg
     7381 cctaaagcat aaaccgataa ccaataccgg tttcagtaat gaaatggcgg gggcgagcgg
     7441 gatcctgttc cagtttttgt cgcagatgcc ccatataaat ccgcaaataa tgactgtgtt
     7501 cgaccgcgtt tggtccccat acctggttaa ggagctggcg ctgggtgagc acttttccag
     7561 cattgttgag cagcaccgcc agcaggcgga attcaatcgg cgtgagatgc acctcttcct
     7621 caccccggtg aatcacgcgg gcggctaaat cgacggtaac atcggaaaat tttaccagcg
     7681 gatcgggcgc ggcggtggcg gagtggcggc gtaatgcgac gcgcagacgg gcctgcaatt
     7741 cgccaatgcc aaacggctta ctcagataat catctgctcc ggcatccagc gcggcgattt
     7801 tgtcgctctc ttcgctacgt gcggaaagca caatcaccgg caccgcgctc cactggcgca
     7861 ggtcgcggat aaactcaatc ccatcaccat cgggcaggcc gagatcgaga ataatcaaat
     7921 ctggcttacg ggtagccgct tccagcaagc cgcgttgcag cgtttcggcc tcatagacgc
     7981 gcatcccgtc gccctccagc gccgtgcgca gaaagcgacg aatagcctgt tcatcttcaa
     8041 caatcagaac gtttgtcaca tatcctcatg aaattcttca agttcagggg cagtttgctg
     8101 gggaagtgta acacgaaaac aggcaccacc ttccggtcgg ttgaacgcgg taatagtgcc
     8161 cccgtgtaca tccactatcg cccgacaaat tgccagtcca agccctaccc ccggtactgc
     8221 cgactcttta ttcccgcgag caaacttatc aaatatcgtc tgctcctggc ctggcggaag
     8281 accggggccg ttatcccaga catccagttg tagattttcg ccctcaacgt gggcatcgat
     8341 accaatttcg gcctgcgcac ccgcatattt caccgcgttc tccagcagat taatcagcac
     8401 ccgttcaaag agtggcccgt caacgtggat taaggtcagc ggttctggca gagaaagatt
     8461 gatgggcgac gataaacccg gttccagcat ttgcagcgcg ctgccgacta cttcttccag
     8521 cgttaaccac tctttcttca aattaaagcc gccggactga attcgcgcca tatccagtag
     8581 attattcacc agtcgggtag tgttcagcac atgctgacga atctcgctgg cctggcgggc
     8641 gtggggtgat ccttcgcttg ccagatcgag cgttaagatt tctgcctgac caaacagcac
     8701 cgtaagcgga gtgcgtaaat catgcgaaag cgccgccagc agggcgttgc ggatctgttc
     8761 acgttcgctt gccatccgcg cctgttcttc gctggcggtc agcgtcagcc gctcaagggc
     8821 attggcgact aacagcgtaa acgtctccag caggcgctgc tgttccggga tcatcaactg
     8881 acgcagattc cccggttcca ccaccaacaa cccgtaggtt ttctcaccgc tttttaacgg
     8941 caaaatctgg tacggcacgc cgggtaacgt gtcggtgccc gcacccgcag gcaggccttt
     9001 atcaaaactc cactgcgcga tggcatcgtc ccacggcgtc attccttgcg gatgtgttaa
     9061 cggctgcaat ttaccgttgt catcgggcaa caacacctga ctgcgggcat gaaacgtgga
     9121 ggcaataaat tgttcgctgg tggcggcgat gtcctgcgga ctgcggccca ccgccagagc
     9181 tttcgacatt tcatataagt gccgtgtgcg ttgctcgcgg taacgggcta cccgtgcctg
     9241 ataacgcaca cccgcagtaa ggttcccgat caccagcccg acggttaaca tcaccgcgaa
     9301 ggtcagcaga tattgcacat cagagacggc gagcgtgccg cgtggggcga taaaaaagag
     9361 atcgaaactc accacattaa tgacggtagc aaccaccgaa ggccagcgtc cataaaatag
     9421 cgccaccacc accacgccaa gtagatacag catcaccagg ttggcggcat caaacgccat
     9481 cagccactgc atggcaatta aggtgataac ggcgcataac gcggcggcaa ccacgcatcc
     9541 ctgaatctgt acccgccact tgtctttaaa agagcggcta tccggcgcgt tgttaatcgt
     9601 gcgggcgggt ggttcatcaa gcgcgaccag cacctgatcg agatcggggg cgatgcgcgc
     9661 cagtcggtca gcaaacgttt cccgacgcca ccagcggcgc gaggctgggc gaccgagaat
     9721 aatcttgccg agattatgtt cacgggcata acgcactacc gctttctctt ccgctggatc
     9781 agaaagtgtt gccgtctccg cgcccagctc ctgcgccaga cgtaaggcgc tgagaattgc
     9841 ccgacgtttt ttctccggta agcgatgcag ggcaggggtt tcaacataca ccgcgtgcca
     9901 gacgctcccc agccgtgacg ccagccgcgc cgccgcgcgg accagttttt cgctgccggt
     9961 gttatggccg atgcataaaa ggatcgcgtc gcgcgtgtgc cagactttct cttcgccagg
    10021 atgcccccgc caggcgcgca tttgctcatc aacgcgatcg gcagtacggc gcagtgccag
    10081 ttcgcgcagg gcgatcagat tgcctttgcg gaaaaaatgt tcaatggcgc gctccgcctg
    10141 cccggcaata tagactttgc cttcttttag ccgctggcgc agatcgtccg ggggcaagtc
    10201 caccagcacc acgtcgtcgg cggcatcgaa aaaaggatcg ggcacggttt cccgtacctg
    10261 aattccggtg acgccgctga ccacatcatt cagactttcc agatgctgaa cgttgacggt
    10321 tgtgaaaaca tcaatgccag cttccagcag ttcttcgata tcctgccagc gtttgggatg
    10381 acgggaacct ggcgcattac tgtgcgccag ttcgtccatt aagatcagcg ccggacggcg
    10441 ggcgagggcg gcatcgagat caaactcgct gatatgccgc ccacggtacg cctggcgttt
    10501 cagcggcaga acagccagcc cttccagcat tgcggcggta tctttccgcc cgtgggtttc
    10561 taccacgcca accacaatat ccagcccttg cgcccgcagt cgctgggctt ctgccagcat
    10621 cgcccaggtc ttcccgacgc ctgcgcaggc accgaaaaaa actttcagct tcccccgatg
    10681 cggcgcggca gtttgttcca gcagacgatc ggggtcggga cgtaagggtt cgttattcat
    10741 caagtttatc cagcgccaga ttgagttcga caatgttgac gaccggctgg ccgatatatt
    10801 tcaccagcgg ttgttggctg tattttgcga tcagttgcgt gagctgttca acgctgagat
    10861 tacgcgcttt cgctacgcgt gggatctgcc aggccgccgc ttgcggggtg atgttattgt
    10921 ccagcccgct tgccgacgcc gtcaccagtt caaccggaac gctcgtgctg gcattcgggt
    10981 tagcggcccg taatgcagca acgcgtgcgg ctattagttt atccagctca gggttactga
    11041 ccgccagatt gctcccgcca gaagcctgtg gattataagg catttctgcc gttgccgacg
    11101 ggcgaccatg aaaatagccg ttgccggtaa aattctgccc gattaatgcc gaaccccgca
    11161 ccgtatcacc ttcacggatc aacgaaccgt tggcctgcca gggaaaccac cattgcccca
    11221 gcgcggtggt cagcagcggg taaacaccgc cagtaatcaa taacagaaag ataaatgtcg
    11281 ataatgccgg acgtaatcca cgcatggtaa acctcacacc agaccgcaaa cggtcagtag
    11341 taaatcaatg actttgatac caataaacgg caccagcagc ccacccagac cgtaaatcca
    11401 taagttacgg cgcaacatgg cagaagcggt aagcggttta taactcacgc cttttaacgc
    11461 caggggaatc aaaaagacga taatcaaggc gttgaaaatg accgcactga gaatggcgga
    11521 gtcgggcgaa tgcaggcgca tgatgttcag cgcatttaac tgtggatacg ttaccgcgaa
    11581 tgccgccgga ataatggcga agtatttcgc cacatcgttg gcaatgctga aggtggtcag
    11641 cgagccacgg gtcatcagca tctgtttgcc aatatgtacc acctcgatca acttggtcgg
    11701 gttagagtcg agatccacca tattgcccgc ttctttcgcc gcctgagtac cggagttcat
    11761 cgccaccgcg acatctgcct gcgccagcgc cggagcatcg ttggtgccgt cgccggtcat
    11821 tgctaccaaa cggccttccg cctgatactg acggatcaat gccagcttgg cctccggtgt
    11881 cgcttcggcg agaaaatcat cgacgcccgc ttccgcggca atggcggcgg cagtcagacg
    11941 gttatcgccg gtaatcatca ccgttttaat acccattttg cgcagttggg cgaagcgttc
    12001 tttaatgccg cctttgacga tatctttcaa cgcaataacg cccagcacac gagaaccttc
    12061 tactactacc agcggtgtgg ccccctgacg cgcaacctga tcgacttttt gatcaacatc
    12121 ggcagggaag tgaccaccgt tagcctcaac atggcggcga atggcatcga cagaaccttt
    12181 acggatcatg cggttgtcga tattgatccc gctcatccgg ctttgcgcag taaacggtac
    12241 aaaggtggca tggagcgact gcacatcgcg ctcgcgcagg ttaaaacgct gcttggcgag
    12301 gatcacaata ctgcggcctt ccggcgtttc atcagccagc gaagccagtt gtgcggcgtc
    12361 agccagcgtt ttttcatcca cgccctgcgc agggataaac tccgatgcct gacggttacc
    12421 gagtgtgatg gtgccggttt tatccagcag cagaacgtca acgtcacctg ccgcttcaac
    12481 tgcacgtccg ctggtggcaa tcacattcgc gcccagcatc cgactcatcc cggcgacgcc
    12541 gatcgctgac aacaggccgc caatagtggt tgggatcaga cagaccagca gcgccaccag
    12601 taccgttacg ctgactgcat taccgcccca cgcggaaaac ggccacagcg tggcggttgc
    12661 cagtaaaaag acgatagtca gggcaatcag caggatggtc agggcaatct cgttcggcgt
    12721 tttgcgtcgc tgtgcgcctt ccaccatcgc gatcatccga tccagaaatg tctcgccggg
    12781 gttaacgcta cactcaatca ccagccagtc agaaagaata cacgtgccgc cggtgacgga
    12841 ggcaaaatcg ccgccggatt cacggatcac cggtgccgat tccccggtga tggcgctttc
    12901 atcgaccgat gcgccgcctt caataacttc accatcgcag gggataatat cgcctgcttc
    12961 taccagtacg atatcgcctt tacgcagttg atcggcggga actttgtccg ccgcagcgcc
    13021 atatttcggt tcgcgcagct tgcgggcaaa ggccgttttt ttcacccctt tcaggctgtt
    13081 ggcctgcgct ttactgcggc cttctgccag cgcctcggcg aaattagcga acagtacggt
    13141 gacccacagc cagccgctaa tggccacgct aaacagcgca ttgccaggca tcgcgccgct
    13201 tgccatcgcg atgctaatac aggtggtcag cagactgccg atccagacga taaacatcac
    13261 cggattgcgc cattgcgcct gcgggtttaa ttttttcacc gcttctttca gcgcctgaac
    13321 gacaagtgtt ggttcgaata gcgccagttg tttacgactc atattcagtg ctcactcaat
    13381 atcatcagga gagatattcc gccaccggac caagcgccag ggcagggata aaggtcagtg
    13441 cgccaaccag caacacggtg ccgattaaca ggccaacaaa cagcgggccg tgcgttggca
    13501 gcgtgccgga gctggcgggt tggctctttt tactcaccag cgaaccggca attgccatca
    13561 ccgggataat caccccgaag cgaccgacaa acatgcagaa cgccagtaaa cagttccaga
    13621 acggagagtt ggcgcttaat ccggcaaagg cgctgccgtt gttgttagcg gcggatgaca
    13681 cggcgtacag cacttcgcta aaaccatgcg ggccagggtt gagcatggcg ctacgtccgg
    13741 cgtcggtcat cattgccagc gccgcgccca tcagcaccag cgtcggggtg accagaatcg
    13801 ccagtgcagt cagtttcatc tcacgtacgt cgattttttt acccagatat tccggtgtac
    13861 gaccaatcat cagcccggca ataaacaccg ccagcaggac aaacagcatc atgccgtaaa
    13921 gaccagaacc gacaccgccg aataccactt caccaatttg catcagccac atcggcacca
    13981 tgccaccgag agcggtaaac gaatcatgca tcgcaatcac cgcgccacag gacgccgccg
    14041 tagtcacgac cgcaaacagg ctactgacca gcacgccgaa acggctctct ttaccttcca
    14101 tattgatgct gctgtctgcg ccgagcgcca gcagatgagg attaccctga acttctgccc
    14161 acatcaccac gcctacgcag atgacaaaaa tcactgacat cgcccacagc aacatgcgcc
    14221 cctggcggcg atcgcccgtc acttcaccaa aggcaaagca cagcgctgtt gggatcaaga
    14281 agatcgccag catctgcacg aagttggtca gtgcggttgg gttttcaaac ggatgcgacg
    14341 agttggcatt aaagaagcca ccgccgttag tgccgagcat cttgatcgct tcctgagaag
    14401 ccactggccc cattggtaac agctgctgcg ctccttcaac ggtcgtcaca gcctgataag
    14461 gctgaaaatt ttgcagtgca ccttgttgaa taaaaaacag cgcgatcaac aacgccacag
    14521 gggccaacac ccataacgtg atgcgtagca gatcgaccca ggcattcccg agtgtgctca
    14581 tgctctggcg ggtaaatgca cggatgaggg aaaaaatcac cgcaatcccg ctggcggcag
    14641 aaagaaagtt ttgcaccgtt aagcccgcca tctggctgaa atagctcaac gtggtttcac
    14701 cgctataaga ttgccagttg gtattagtga caaagctgac ggcagtattc agcgccagat
    14761 cccacgacag ccccggcaac tgctgtggat taagcggcag atagtgctga ccgagcagca
    14821 tgaaaaaaag caccgccagc cccagcatgt tcagacagag aatggctgag agatactgct
    14881 tccagttcat ctcacggtca gagacgccaa gcgcgctaaa gagtacgcgt tcaacgcccg
    14941 ctgtaccggg aagaggaata tcattaatca accgtgccag cccgctgccg aaaggacgcg
    15001 ccagcaccat taataccagt aaaaacgtgg caatcagtaa gaacccttgc gcagccatca
    15061 gaacgcctcc gcattgatca gggcataaac cagataaccc agtaataaaa acaccagcaa
    15121 tacgccggtt atcacgcctg cactcacagt gcacctccag tggcctaaaa gtgataccgg
    15181 aagggtagaa ttctggctgc aaagatttcg caaaaatctg cggacggggt gtaaaaaaag
    15241 tataaaaatg gcaaaagcca tgatttaact aatgtttagt attaatttaa cttttgtgta
    15301 acttaattac agggtgaatg taaataaacc atcaataagc aaaaataagt ggtcggatga
    15361 gtagtaaaat tacacaaaag gcggtactat tttcatcaga taaacaaatt catttttccg
    15421 gtgctattca ccgggtagat acaaaagggg gagaaaagta tggaactcta cagagaatat
    15481 cctgcatggc ttatcttttt acgccgtact tatgcggttg cagcgggcgt tctggcgctg
    15541 cctttcatgc ttttctggaa agaccgcgcc cgcttttaca gctacctgca tcgcgtctgg
    15601 tcgaaaacca gcgataaacc ggtgtggatg gatcaggccg aaaaggcaac cggtgatttt
    15661 tattgattgc tgagttctga atacattgaa aaccgccagc ggaaacgctg gcggtttttt
    15721 gtatcagga
//
LOCUS       DAZGCQ010000107        15440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000107 DAZGCQ010000000
VERSION     DAZGCQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.107"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            206..1063
                     /gene="focA"
                     /locus_tag="ACHK07_003952"
     CDS             206..1063
                     /gene="focA"
                     /locus_tag="ACHK07_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO8104226.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1118..3400
                     /gene="pflB"
                     /locus_tag="ACHK07_003953"
     CDS             1118..3400
                     /gene="pflB"
                     /locus_tag="ACHK07_003953"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8104227.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3592..4332
                     /gene="pflA"
                     /locus_tag="ACHK07_003954"
     CDS             3592..4332
                     /gene="pflA"
                     /locus_tag="ACHK07_003954"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO8104228.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4431..4541
                     /locus_tag="ACHK07_003955"
                     /pseudo
     CDS             4431..4541
                     /locus_tag="ACHK07_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4542..5972)
                     /gene="ycaM"
                     /locus_tag="ACHK07_003956"
     CDS             complement(4542..5972)
                     /gene="ycaM"
                     /locus_tag="ACHK07_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8104229.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIHNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6182..7330)
                     /gene="ycaD"
                     /locus_tag="ACHK07_003957"
     CDS             complement(6182..7330)
                     /gene="ycaD"
                     /locus_tag="ACHK07_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104230.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7645..8271
                     /gene="ycaC"
                     /locus_tag="ACHK07_003958"
     CDS             7645..8271
                     /gene="ycaC"
                     /locus_tag="ACHK07_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO8104231.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(8307..9170)
                     /gene="dmsC"
                     /locus_tag="ACHK07_003959"
     CDS             complement(8307..9170)
                     /gene="dmsC"
                     /locus_tag="ACHK07_003959"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO8104232.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9172..9789)
                     /gene="dmsB"
                     /locus_tag="ACHK07_003960"
     CDS             complement(9172..9789)
                     /gene="dmsB"
                     /locus_tag="ACHK07_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO8104233.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(9800..12244)
                     /gene="dmsA"
                     /locus_tag="ACHK07_003961"
     CDS             complement(9800..12244)
                     /gene="dmsA"
                     /locus_tag="ACHK07_003961"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO8104234.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTREEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(12544..13536)
                     /locus_tag="ACHK07_003962"
     CDS             complement(12544..13536)
                     /locus_tag="ACHK07_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8104235.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            complement(13606..13953)
                     /locus_tag="ACHK07_003963"
     CDS             complement(13606..13953)
                     /locus_tag="ACHK07_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8104236.1"
                     /translation="MNSTLSISIGEKILMIRKSERLTRKQFFELTGIPVNTQRYYETG
                     RQDGIGSEILLKITKNLRFKKYALWLTTDETFEAGGQIAPALSLDGSSQSEGDLASID
                     VTQKSSRSGRNAG"
     gene            14055..14579
                     /locus_tag="ACHK07_003964"
     CDS             14055..14579
                     /locus_tag="ACHK07_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8104237.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            14631..14825
                     /locus_tag="ACHK07_003965"
     CDS             14631..14825
                     /locus_tag="ACHK07_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104238.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            14851..15186
                     /locus_tag="ACHK07_003966"
     CDS             14851..15186
                     /locus_tag="ACHK07_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8104239.1"
                     /translation="MKQQRNARINNNTERYVARQHFRKNSATVTTEIHATPDGHPVKQ
                     TGKHTWTIGDTGIVIHKSFRSPITGRFNFTLTRGDDYFGQDFTFFEALRTADRLINGL
                     RFQYPGSKH"
     gene            15205..>15440
                     /locus_tag="ACHK07_003967"
     CDS             15205..>15440
                     /locus_tag="ACHK07_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO8104240.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVA"
BASE COUNT         4202 a         3972 c         3606 g         3660 t
ORIGIN      
        1 tagcggagga tgcggaaaaa attcaactca tttgttaatt tttaaaattt atttttattt
       61 ggataatcaa atatttactc cgtatttgca taaaaaccat gcgagttacg ggcctataag
      121 ccaggcgaga tatgatctat atcaatttct catctataat gctttgttag tatctcgtcg
      181 ccgacttaat aaagagagag ttagtgtgaa agctgacaac ccttttgatc ttttacttcc
      241 tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat aaagcaacga aacatccgct
      301 taagactttc tatctggcga ttaccgccgg tgttttcatc tcaatcgcat tcgtcttcta
      361 tatcacagca accactggca caggcacaat gcccttcggc atggcaaaac tggttggcgg
      421 catttgcttc tctctggggc tgattctttg tgttgtctgc ggagccgatc tctttacttc
      481 cacagtgttg attgttgttg ctaaggcgag tgggcgcatc acctggggtc agttggctaa
      541 aaactggcta aatgtctatt ttggcaacct ggtcggcgca ctgctgtttg tacttttaat
      601 gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg ggactaaacg tcctacaaac
      661 cgccgaccac aaagtgcacc atacttttat tgaggccgtc tgccttggta tcctggcaaa
      721 cctgatggta tgtctggcag tatggatgag ttattctggc cgcagcctga tggacaaagc
      781 gtttattatg gtgctgccgg tcgcgatgtt tgttgccagc ggttttgagc acagtatcgc
      841 aaacatgttt atgatcccga tgggtattgt aatccgcgac ttcgcatctc cggaattctg
      901 gaccgctgtc ggttctgcac cggaaaattt ttctcacctg accgtgatga acttcatcac
      961 tgataacctg attccggtta cgatcggtaa tattatcggc ggtggtttgt tggttgggtt
     1021 gacatactgg gtcatttacc tgcgtgaaaa cgatcaccat taatggttgt cgaagtacgc
     1081 agtaaataaa aaatccactt aagaaggtag gtgttacatg tccgagctta atgaaaagtt
     1141 agccacagcc tgggaaggtt ttaccaaagg tgactggcag aatgaagtaa acgtccgtga
     1201 cttcattcag aaaaactaca ctccgtacga gggtgacgag tccttcctgg ctggcgctac
     1261 tgaagcgacc accaccctgt gggacaaagt aatggaaggc gttaaactgg aaaaccgcac
     1321 tcacgcgcca gttgactttg acaccgctgt tgcttccacc atcacctctc acgacgctgg
     1381 ctacatcaac aaagcgttgg aaaaagttgt tggtctgcag actgaagctc cgctgaaacg
     1441 tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt tcctgcaaag cgtacaaccg
     1501 cgaactggac ccgatgatca aaaaaatctt cactgaatac cgtaaaactc acaaccaggg
     1561 cgtgttcgac gtttacactc cggacatcct gcgttgccgt aaatccggtg ttctgaccgg
     1621 tctgccagat gcttatggcc gtggccgtat catcggtgac taccgtcgcg ttgcgctgta
     1681 cggtatcgac tacctgatga aagacaaata cgctcagttc acctctctgc aggctgatct
     1741 ggaaaacggc gtaaacctgg aacagactat ccgtctgcgc gaagaaatcg ctgaacagca
     1801 ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac ggctacgaca tctctggtcc
     1861 ggctaccaac gctcaggaag ctatccagtg gacttacttc ggctacctgg ctgctgttaa
     1921 gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc accttcctgg atgtgtacat
     1981 cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa gcgcaggaaa tggttgacca
     2041 cctggtcatg aaactgcgta tggttcgctt cctgcgtact ccggagtacg atgaactgtt
     2101 ctctggcgac ccgatctggg caaccgaatc tatcggtggt atgggcctcg acggtcgtac
     2161 cctggttacc aaaaacagct tccgtttcct gaacaccctg tacaccatgg gtccgtctcc
     2221 ggaaccgaac atgaccattc tgtggtctga aaaactgccg ctgaacttca agaaattcgc
     2281 cgctaaagtg tccatcgaca cctcttctct gcagtatgag aacgatgacc tgatgcgtcc
     2341 ggacttcaac aacgatgact acgctatcgc ttgctgcgta agcccgatga tcgttggtaa
     2401 acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa accatgctgt acgcaatcaa
     2461 cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg aagtctgaac cgatcaaagg
     2521 cgatctcctg aactacgatg aagtgatgga gcgcatggat cacttcatgg actggctggc
     2581 taaacagtac atcactgcac tgaacatcat tcactacatg cacgacaagt acagctacga
     2641 agcctctctg atggcgctgc acgaccgtga cgttatccgc accatggcgt gtggtatcgc
     2701 tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa tatgcgaaag ttaaaccgat
     2761 tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa ggcgaatacc cgcagtttgg
     2821 taacaacgat ccgcgtgtag atgacctggc tgttgacctg gtagaacgtt tcatgaagaa
     2881 aattcagaaa ctgcacacct accgtgacgc tatcccgact cagtctgttc tgaccatcac
     2941 gtctaacgtt gtgtatggta agaaaacggg taacacccca gacggtcgtc gtgctggcgc
     3001 gccgttcgga ccgggtgcta acccgatgca cggtcgtgac cagaaaggtg ctgtagcgtc
     3061 tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa gatggtatct cctacacctt
     3121 ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt cgtaagacca acctggctgg
     3181 tctgatggat ggttacttcc accacgaagc atccatcgaa ggtggtcagc acctgaacgt
     3241 taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa aatccggaaa aatatccgca
     3301 gctgaccatc cgtgtatctg gctacgcagt acgtttcaac tcgctgacta aagaacagca
     3361 gcaggacgtt attactcgta ccttcactca atctatgtaa ttagatttga ctgaaatcgt
     3421 acagtaaaaa gcgtacaata aaggctccac gaaagtgggg ccttttttag cacgagagcc
     3481 ttttttgtca gctatctata ctttaaggtg actgccaaaa cagactcgac gtagccttcg
     3541 agctgcgcac caacacggcc tcagatgggc cacatctgga gaaacaccgc aatgtcagtt
     3601 attggtcgca ttcactcctt tgaatcctgt ggaaccgtag acggcccagg tattcgcttt
     3661 atcacctttt tccagggctg cctgatgcgc tgcctgtatt gtcataaccg cgacacctgg
     3721 gacacgcatg gcggtaaaga agttaccgtt gaagatttga tgaaggaagt ggtgacctat
     3781 cgccacttta tgaacgcttc cggcggcggc gttaccgcat ccggcggtga agcaatcctg
     3841 caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa aagaaggcat tcatacctgt
     3901 ctggacacca acggttttgt tcgtcgttac gatccggtga ttgatgaact gctggaagta
     3961 accgacctgg taatgctcga tctcaaacag atgaacgatg agatccacca aaatctggtt
     4021 ggagtttcca atcaccgcac gctggagttc gccaaatatc tggcgaataa aaatgtgaag
     4081 gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg atgacgattc agcgcatcgc
     4141 ctcggtgaat ttacccgtga tatgggtaac gttgagaaaa tcgaacttct cccctaccac
     4201 gaactgggca aacacaaatg ggtggcgatg ggtgaagaat acaaactcga cggtgttaaa
     4261 ccaccgaaga aagaaaccat ggaacgcgtg aaaggcattc ttgagcagta cggtcataag
     4321 gtaatgttct aatttccccg taaagcggca actttattga gttgccgctt ttttattccg
     4381 cgctgcataa ataattcaga gctctatttc taacgccctt acagcacagc ttccaaatgt
     4441 attacccaaa ccgccagtat atgtcctgcg gcttgcgcgc atttctcgat tatgtaaaaa
     4501 cgggtcagat ctgccagagt cagcgtcacc gaccacaata attatttact attcgcttta
     4561 cgcgccagca gcgggaatat cagtcctaat cctaccagca caaacggcgt tgcaacattc
     4621 agcgccaact ggaaggtcca ctctgcagtg aaggcttcca ttttcgggaa gatccccgtc
     4681 aaacaggcaa aggcggtaaa ggcaaaacac caaatcccga cggtcattgc cagaggcttg
     4741 ttatgaataa agacatattc tggtttatat ttctgcgcca ggcgaacgac tgcaataaat
     4801 gcaacaaata cccataaata acgcagcggc atcactaccg agttaagatt caacagccat
     4861 ttatagagat tgttcatatc accgatgccg agagtcggca gcataatcag gatcgccacc
     4921 agcaccaggg tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa
     4981 ctggctggaa tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc
     5041 gagaatacca gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa
     5101 gtattaccca tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttagtc
     5161 attaagtcat ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga
     5221 atggcacaaa ccgcaaccat caccgccagg cataacatcc cttttggaaa ttcttttcct
     5281 gggttgcgcg tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc
     5341 agcattgaaa tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta
     5401 atgtttgtgg tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca
     5461 tacaggagcg acataacaaa catcgcaatc cctgccacag aaccgacgat tttcagcgat
     5521 ttcataccgc gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt
     5581 aacgctacga ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc
     5641 gcaatcagta ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc
     5701 cacgcggcga ggtaagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg
     5761 cctttcccat ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa
     5821 tagagcgcaa agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta
     5881 ttaacaacgt tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat
     5941 cgcagctgtt tttcctgaac attcccagcc atagacaaat ccttcgccaa aattaaaggg
     6001 gcatctgtaa aacgagggat tatgcgcggc aaataagaag tgcggaaata gcgaaatgca
     6061 aaattataac ctgctgcaca ttttattttt aaacgaataa aatttggaaa aaagcaagtc
     6121 agatagaaga aacaaaaaag cagagtgtga aacagtaagt tcacactctg cttgaattca
     6181 tttacacgtg agcaacgggt ttcggcgtat gaccggcgtt gcgaagcagc atcagcaaat
     6241 agataaacga tacgctggcg atcatgataa acaataaatt atcggagaaa ttctgcatta
     6301 gcatagcggt aaatgacggg ccaagcagac ttcccacggt atagctcaac agtaaggcct
     6361 ggttcatcgc caccagttga tgatgttcaa ctttctcgca agcccaggcc atcgccaccg
     6421 gatatagcgt aaagccagcg gcaccgagga tgaataacgc tggggccatc gccgcctggc
     6481 taagcatcgc gatactgccg agaatgacga caaaaacctg aacacgcaac accagcagtc
     6541 gaccaaactt atccgccaga cgtccaatcg gccattgtcc gaggataccc gcactgacca
     6601 gtaccgccat ccagaaacca atgctggcat tactcacccc cttgtgattg aggtacaacg
     6661 gcatcaggcc atatagagat cccagaacga ttcctgagat aatgcagcca ttcacgccaa
     6721 gccgcgcctg acggagtttt agcattgacg taattgacgt cgaatcatgg ttttcagcct
     6781 gctgattcag gacacgcgta aacaacagcg gtaagatccc tgccaacgtc aaacctgtaa
     6841 cccacggcaa tacgctcatc agctcggttg agactttgct gaccagtaac tggcctaaaa
     6901 acgttcccac gtaataaacc atcatatacg cagcaagcaa acgcccacgg ttacgtgacg
     6961 tcccactgca catcagcgcg ctctcaacga ctacccaaat catggcacag ccgacgcccg
     7021 cgacaaaacg ccacgccaac cagctccaga aaccaatcat caggccaagg cccgcacagc
     7081 cagcggcaaa aatgaaggag gccagataat agctgcggtt aaagccaatg cgtttaatga
     7141 cataccctgt cagcaatgta ccgacaaggt tgccggtaaa ataggatgag ctgacaacgc
     7201 ctacctgcca tgtggacatg tgttcctggg cgagccaaag cggcacgagt gtatttaaca
     7261 ccgcgatcgc cagagtcaac aaaagcaggc cagacagcaa aagcatgaca ggccgggtat
     7321 acgtggacat gaattaaaac cgtgaggaag ttcaaatttc atgcgcatca tgccaccgcc
     7381 aaaaacattg tcaatgggtg aaaatacgcg catatcgcaa cggcggtatt atcgatttat
     7441 attactcatt cataacgcta atactgaaga gatgattaat ctcatctgat tgtttttatt
     7501 gatctaacac aaatttcata cgcttcgttc agtcgaaaaa tttcatggtc acagcaaact
     7561 catatttccc gtctatgctt tcagagcaat gcatcgcgtc tgaccgattt cagacgtacc
     7621 actgactctc gaaaggagaa gaggatgacc aaaccgtatg ttcgtcttga taaaaatgat
     7681 gctgccgttt tgcttgttga tcaccaggct ggtttacttt cccttgtacg ggatatcgaa
     7741 cccgataagt ttaaaaataa cgtgctggcg ctgggtgacc tggctaagta tttcaactta
     7801 ccaactattc ttaccactag ttttgaaacc ggtcctaacg gcccattagt tccagaactg
     7861 aaagcacaat tccccgatgc accttacatt gctcgcccgg gcaatattaa cgcctgggat
     7921 aacgaagatt ttgtaaaagc agtcaaagcg acaggtaaaa aacagttaat tattgccggt
     7981 gtggtaaccg aagtttgcgt ggcattcccg gcgctttcag ccattgaaga agggtttgat
     8041 gtcttcgttg tgactgacgc gtcagggacc tttaatgaaa ttacccggca ttcggcatgg
     8101 gatcgcatgt cgcaagcagg tgcgcaattg atgacatggt tcggcgtggc ttgcgagctg
     8161 caccgtgact ggcgtaatga tattgaagga ctggcgacgt tgttctctaa ccatattcct
     8221 gattatcgta atttgatgac cagttatgac acgttaacga agcagaaata aaagagaacc
     8281 ggggccgaaa ccccggttgt tactgattag cttgcgacgg ccatccccac ggtcatatgc
     8341 aaaccataga atacgccgcg accgattaat tcccctgcca gaagcagaat gaatgagaca
     8401 gatagtagcg gaaccgcagg ctgataacct tttagctgtg gtgcaatcca caagcacagg
     8461 gcaacagcca aaagcacgat ccgccaggac atcagcgcac catagtccgg caccagcgct
     8521 gcggcctgct gcacagaact atgaatagtc gccagctctg cgccctgcat cactgacatc
     8581 acgccactca ctaccagtgc aagtacagaa atggctggca gcagacgcat cgcccagcca
     8641 tcgacgccag ccagactcag caacaggtaa cccagtaacg ggccgcccat aaacattgtc
     8701 aggaagaagc ccatcggcgt ccagatgctg taccaggtcg gaacggtatc aatgctgtta
     8761 tagacacgca ccatcatcca gacaaagact acgccaagaa ccatcgtcac taccagccac
     8821 agcgtacgca atgccggtga cagctttttc agcattgcca gcaaccagcc gatgccgcct
     8881 acggcaaaaa agatggaacc gctggcgatt tcgttactga gtgctgaagc ccctacccgg
     8941 ttgagcgagt taaaggcgcg catcggtgaa ccaagatgga gcatagaggc gataaagcca
     9001 atgcccatca gcacccataa gccaaacatg caggcgataa cacgctgctg ggcttctgct
     9061 cgcaggtcgc ctttgagcag cgccaaagcc agaacgataa aaccacccgc tacacattgc
     9121 ccgaagaccg tgaagatcat cagcggccat tcatgccatc cacttcccat ctcacacctc
     9181 cttcgggttt gccagatagc cagtggtatc cccggtcggg cggctattgg cattgggttt
     9241 gatcacaata ttcggtttgg taaagtgagc tcgcggcaac ggcgcaacgg ccgccagatc
     9301 gccatgtttt ttacgcagct cgtcgatagg gccgaaatcc agcgcccgca gcggacaaga
     9361 ttcaacacag atcggctttt taccctccgc aacacggtca taacaaccat cgcatttggt
     9421 catatggcct ttcgtttcgt tatattgcgg tgcgccatac gggcaagcca tatggcagta
     9481 gcggcagcca atgcacacat cttcatcgac cacaacaaaa ccatcttcac gtttatgcat
     9541 cgcaccgctc gggcagactt tagtacaagc cggatcttcg cagtggttgc acgaaatcga
     9601 cagatagtag gcaaacacgt tctggtgcca gacaccgtta tcttcctgcc agtcgccacc
     9661 agcatattca taaatgcggc ggaagctgac ttctggtgtc aaatctttgt agtctttaca
     9721 agccagctcg caggttttgc aaccggtgca acggctggaa tcaataaaaa atccatactg
     9781 ggttgtcatc ggttactcct tacacctttt caacctgaac aaggtttgta tgtgacggat
     9841 tccccttagc gagaggagac ggacgttgag tggtcagtac gttaatacaa ccacccttat
     9901 cgacacgttt tgcatccggg tcataccagg caccttcacc cagtgcgacc acacccggca
     9961 tcattcgtgg cgtcactttc gcctcaatat gaacctcacc acgatcgtta aagatcctga
    10021 ctttatcgcc gttgtggata ccgcgttttt gggcatcaag cgggttgatc cacatttcct
    10081 gacggcaagc cgctttcagc acatcaacgt tgccgtaagt tgagtgaacg cgagatttat
    10141 agtggaaacc tgtaagctgc agcggatact gtttgttcag cggatcctga taactttcaa
    10201 agcccggcgt gtagatcggc agtggatcga tcacatcgcc ttctggcaat tcccaggtag
    10261 cggcaatgtc agccagcgcc tgcgaataaa tctcaatttt acccgatggc gtggtcagtg
    10321 gatttgcctg cggatcttca cggaaggctt tataagcaac gtgatgccct tgtgggtcgc
    10381 gctttttaaa gatcccctgc ttgcggaact cttcaaacgt tggcagttca ggaatcgctt
    10441 cccgcgactg ggcatacaga tgccgcatcc actcttcccg ggtacggcct tcagtaaact
    10501 gttgctcaac gccaagacgt tttgccagtt cgctggtcat ttcatagatg gtcttacatt
    10561 caaagcgcgg tttaatcacc tgatcgttga aaatcacgta agacatattc ccgcaggatg
    10621 catccagtgc aaagtccatc tgttcggaag cggtacagtc aggcagcagg atgtcagcat
    10681 atttcgccga tgaagtcatg tggcagtcga taaccacaat cagctcgcac ttcttatcat
    10741 cctgaaggat ttcatgggta cggttgattt cagaatgctg gttaatcagg cagttaccgg
    10801 catagttcca gatcattttg atcggcacat ccagcttatc tttcccgcgc acaccatcac
    10861 gcagcgccgt catttccggg ccacgttcaa tggcatcggt ccacataaac atcgaaatgc
    10921 tggtctggat cgggttttcc aaggtcggca tacggacaaa cggtaagctg tatgaacctt
    10981 cacgcgcgcc gctgttccct ccattaatac caacgttacc ggtcagaatc gccagcatcg
    11041 agatagcacg ggttgcgatt tcaccgttag cgtgacgctg cgggccccat ccctggctga
    11101 taaacgccgg tttggtactg ccgatttcac gagccagttt gatgattttg tctgccggaa
    11161 caccggtgat ttgcgaggcc cattccggtg ttttagccac gccatctggc ccttcaccca
    11221 gaatataagc tttatagtgg ccatttttcg gcgcactggc tggcagggtt ttctcgtcat
    11281 agccaacgca atatttatcg aggaatgcct gatccaccag gttttcggtg atcatgacgt
    11341 acgccagacc gttaaccagt gcggcatctg ttcctggacg aatagggatc cactcatctt
    11401 cgcgcccggc accggtgtcg gtatagcgcg gatcgatgat gatcatgcgg gcattagatt
    11461 tctggcgtgc ctgttcaaga tagtaagtca ccccaccgcc actcattcgc gtttcgccag
    11521 ggttattacc aaacagcact accagcttac tgttttcgat atccgacggg ctgttgccat
    11581 ctgcccagcc gccgtaggta tagttcagac cttccgcaat ttgcgcggag gagtagtcgc
    11641 cgtaatggtt cagatagccg ccgcagcagt tcatcagccg cgcgaccagg gtatttcccg
    11701 gcggccagga gcgggtcatg gtgccaccca gcgtaccggt gccatagtta agatagatgg
    11761 attcgttgcc gtactctttg atcaggcgct gcatattggt cgcgatgatg tcgtaggctt
    11821 cttcccagct aatgcgctcg aatttgcctt caccgcgcgc cccgactcgt ttcatcggat
    11881 atttcaggcg gtccggatta tagacacggc gacgcatgga acgcccacgc aaacaggcgc
    11941 gaacctggtg caggccgtcg taattgtcat cgccggtatt gtccgtttcg acatatttga
    12001 tttcaccgtc cacgacgtgc atacgtagcg ggcagcgact accacagtta actgtacagg
    12061 cgctccagat aaccttttcg tctgattttg ttggaatggc gctatcgaca gcgtgcgcaa
    12121 tccgactaaa aggtaatgtt aatgcgctgc tggccattgc caggccgccg atcgctgtcg
    12181 tttttaccaa accacggcga ctcacctcag cagccaatac cgcatcaggg attttcgttt
    12241 tcataatggc tcactcaagc ttgctcacga ttgattccag taacaaaccg tattgtgtaa
    12301 ttatttctat agcagatcgc tatataggct tgtatacatc gaataatttg aaaatcaaag
    12361 agataggtag attttcactc gaaagtagta ttacgtgtaa gtgaggagta attattgttc
    12421 ggtatcaaag ggagacaaaa aaagcgcccg caggagattt aacacacctt actccccttg
    12481 atgatttact tatttttcaa tgactaaatc aaaacaaggg gagcaaatta acgcaaatta
    12541 caactagtca atggacagtt tatggacact atcagccagc ggattaaagc taatcgcatc
    12601 ctgtaaaaag tccggtgaaa aatgcgcata cgtcatcgtt tgttgaatcg tggcgtgccc
    12661 aaggattcgc tgtaacgtaa cgatatttcc gccgttcatc atgaaatgcg tggcgaacgt
    12721 gtgacgcata acgtgagtcg cctgaccttt gggtaaatcc ggtttcacct cctgaaggat
    12781 atcccgaaaa cggatgtaat cagcctggta caatagcccg gtttttcggg tttttatcaa
    12841 cgaaagcacc gtatcagaaa caggaaccgc acgggatttt ccattctttg tgttaaagaa
    12901 cattacgcgg ttaccaacga tatgctcacc acgtaaacca gctgcttccc cccaccttgc
    12961 gccagtggaa agacacagaa cagcaacgcg acgcgcatcc ccctcgagtc gttcaagcag
    13021 ccgatcaatc tcatcatctg aaagaaaggc catttctgta ttcctgacct tgagcttacg
    13081 tattccacgc accgggtttt cgttatggaa aacctccgca tcaatcagcg aggtaaacat
    13141 tgcagacaag gcacacaaat cacgattaac actggagggc attaatccgg cctgtaattt
    13201 atctgaacga tacccgagca taaactttcg cgtcatctca ctggcgcggg ggtccctcat
    13261 ctcacggttt attttttcca gcctgaccct gtaagtatcc ccgtaaggct gattccggcc
    13321 atcaagcacc caccaggcat ctatcagttc ggataaccga cgctgatcag ctggtttggc
    13381 ctgccacggt ttatcgtgga aattctgcaa aacatacctt tcaaactcct gtgcctttga
    13441 cttcagggta aaaatcttac ggatccgctt tccttccgtc ccttgcggtc ttacatcaac
    13501 cttataacgc ccgtctttga gttgcttaat cgacatgatt agcccctcca acggatgaat
    13561 cagccgtaca ctctctacgg gatgaaaccc gctcgatgtg taacgtcagc cagcattgcg
    13621 gcctgatcgg gatgattttt gggtaacgtc gattgaagcc agatcgccct ccgactgaga
    13681 agatccatca agagagagag ccggggcaat ttgaccccca gcttcaaacg tttcatcagt
    13741 tgttaaccaa agtgcatatt ttttaaaacg taaatttttt gtgatcttta ataagatctc
    13801 acttccgatt ccatcttgcc ttcccgtttc gtaatacctc tgcgtattaa caggaatccc
    13861 tgtaagctca aagaattgct tccttgtaag cctctcactt ttccggatca tcaagatttt
    13921 ttcaccgata cttattgaca gtgtcgaatt cataccacta aaatcctcgc aaaagtgtcg
    13981 taatttcgac atcaaacaaa tcaaaacaaa ccttcatgag gcgcgacaag cgcaccaacg
    14041 caagaggata gcaaatgcgg aagtcaattg ccgataaaga gttatctgac acacaagccc
    14101 ttcaggaaaa cgacagcatc accgaatccg ttggtgcggc aaacgacgat gaacaccaag
    14161 aggaaaagaa aaaacgcacc tacaaaaaag gggcaacctt gcgactggat ggcccggtag
    14221 caggactttg ctcacttgaa aaaggggcag cctatatcgg gttgacaaaa gctgcattgc
    14281 gcgtggcgct tcagcgaaat cagatgcctg gacacaagac ccgttcaaac cctgaagatg
    14341 aaaattcgga tggcgtatgg tggtttaacg ccagagagtg ggacaagctg gctgacgaac
    14401 taccagaatg cgagccgcca gaatggcaca actggaaaag ctactggacg tacgaccgac
    14461 agaaaagaaa gttttccccg gcaaacaaag aagactgcca gaccatcaat ggaaagcggg
    14521 tttacacagg aagaaagtca aaactggagc aacaccaata cagcgaaggc aaccagtgac
    14581 atcaaaacaa accgggatca ttaagagcat cgggatttga aggtgttgac atgactgact
    14641 ccactcaaac aaaagactta tacagcgatt tacgattcat catcatacaa cagcaatcag
    14701 gcaggatgga tccaacacgt gcaagcaagg cctggaaaga agcccttacc gatcagggga
    14761 tcgatgtaat tagcgaagtc atggctgaag tgatttttag gctaacaaga gacgaatcac
    14821 tgtaatcatc gcataaggac cgagtgaaga atgaagcaac aacgaaatgc acgcatcaat
    14881 aacaacaccg aacgctacgt tgcacgccag cacttccgta aaaatagtgc gaccgtaacc
    14941 actgaaatcc acgcaacacc agacggccat ccggtaaaac aaaccggcaa gcacacctgg
    15001 acgattggcg acactggcat cgttattcac aaaagttttc gcagtcctat tactggcaga
    15061 tttaatttca ccctgactcg cggagatgat tacttcggac aggatttcac attttttgaa
    15121 gccttaagaa cggcagacag attaatcaac ggcctgcgtt ttcaatatcc gggaagcaag
    15181 cactgataaa taaaggattc agccatgatc gaagcctaca aaatacttat taataacgcg
    15241 ttgcagcgtt accacttcga acttgcaaac cggggcgtaa ctaaggatga aagccttcgt
    15301 aactcactgc gcggcgcaat tatgggactg tataactgcg cgctgggcag cgatgaccgt
    15361 gaagcgctgg aagaattaaa gtcgattatg acagaactgt ttgaaggtga aattgtcgag
    15421 ccttacttca caggagtggc
//
LOCUS       DAZGCQ010000108        15366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000108 DAZGCQ010000000
VERSION     DAZGCQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.108"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..390)
                     /gene="yhaJ"
                     /locus_tag="ACHK07_003968"
     CDS             complement(<1..390)
                     /gene="yhaJ"
                     /locus_tag="ACHK07_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8104241.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAII"
     gene            495..1196
                     /gene="yhaK"
                     /locus_tag="ACHK07_003969"
     CDS             495..1196
                     /gene="yhaK"
                     /locus_tag="ACHK07_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO8104242.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLRKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1219..1383
                     /gene="yhaL"
                     /locus_tag="ACHK07_003970"
     CDS             1219..1383
                     /gene="yhaL"
                     /locus_tag="ACHK07_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO8104243.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1517..2827)
                     /gene="cyuA"
                     /locus_tag="ACHK07_003971"
     CDS             complement(1517..2827)
                     /gene="cyuA"
                     /locus_tag="ACHK07_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO8104244.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVVEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2855..4186)
                     /gene="cyuP"
                     /locus_tag="ACHK07_003972"
     CDS             complement(2855..4186)
                     /gene="cyuP"
                     /locus_tag="ACHK07_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8104245.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4461..5825)
                     /gene="tdcG"
                     /locus_tag="ACHK07_003973"
     CDS             complement(4461..5825)
                     /gene="tdcG"
                     /locus_tag="ACHK07_003973"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8104246.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5897..6286)
                     /gene="tdcF"
                     /locus_tag="ACHK07_003974"
     CDS             complement(5897..6286)
                     /gene="tdcF"
                     /locus_tag="ACHK07_003974"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO8104247.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6300..8594)
                     /gene="tdcE"
                     /locus_tag="ACHK07_003975"
     CDS             complement(6300..8594)
                     /gene="tdcE"
                     /locus_tag="ACHK07_003975"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO8104248.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8628..9836)
                     /gene="tdcD"
                     /locus_tag="ACHK07_003976"
     CDS             complement(8628..9836)
                     /gene="tdcD"
                     /locus_tag="ACHK07_003976"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO8104249.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9862..11193)
                     /gene="tdcC"
                     /locus_tag="ACHK07_003977"
     CDS             complement(9862..11193)
                     /gene="tdcC"
                     /locus_tag="ACHK07_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO8104250.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11215..12204)
                     /gene="tdcB"
                     /locus_tag="ACHK07_003978"
     CDS             complement(11215..12204)
                     /gene="tdcB"
                     /locus_tag="ACHK07_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO8104251.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12303..13241)
                     /gene="tdcA"
                     /locus_tag="ACHK07_003979"
     CDS             complement(12303..13241)
                     /gene="tdcA"
                     /locus_tag="ACHK07_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO8104252.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            13556..13774
                     /gene="tdcR"
                     /locus_tag="ACHK07_003980"
     CDS             13556..13774
                     /gene="tdcR"
                     /locus_tag="ACHK07_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO8104253.1"
                     /translation="MSKFSNFIINKPFSAINNAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            14030..14569
                     /gene="yhaB"
                     /locus_tag="ACHK07_003981"
     CDS             14030..14569
                     /gene="yhaB"
                     /locus_tag="ACHK07_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO8104254.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            14591..>15366
                     /locus_tag="ACHK07_003982"
     CDS             14591..>15366
                     /locus_tag="ACHK07_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104255.1"
                     /translation="MFPVSSIGNDISSDLIRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRN
                     VDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESCLNELGDITEEQLKIMRFAIIES"
BASE COUNT         4180 a         3928 c         3483 g         3775 t
ORIGIN      
        1 gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc
       61 aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag
      121 agcttccgca tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccaa
      181 caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac
      241 atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag
      301 ctcatccgct gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc
      361 ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac
      421 cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag
      481 agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg
      541 gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag
      601 gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc
      661 cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc
      721 gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg ctctctaccc
      781 agccgggtgt cagctacagc gaacacaatc tccgcaaaga caaaccgtta acacgaatgc
      841 agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa ctggcgctta
      901 atatgggcaa gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac
      961 gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt
     1021 tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc
     1081 atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac gaggctaaca
     1141 ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt
     1201 ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc agtgaagccc
     1261 gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc
     1321 gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg
     1381 taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa tccaacaaca
     1441 ttaacgaatt aaacaacccg taggccggat aagatgcgcc agcatcgcat ccggcacgat
     1501 acccaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc tgccgatccg
     1561 tttgctgcat cgaatggctt gctaatgcac acaggttggc aatcgactgc tcaacatcat
     1621 gcgccacaat cccttcattg ccggtcacgg cggtatcatc cagcgccatt aacaccgctt
     1681 tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac gcaccatcgc
     1741 aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagatg gtttcataac
     1801 gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc gttgcggcac
     1861 acagcgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa tgagaaagca
     1921 tcagcgcacg cgccagccgt tcatcatccg ctccgaagtg ttctgctacc accaccacag
     1981 gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc ggaagcgtag
     2041 cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat gaagagagat
     2101 ctttcgccag caaaccgcgc gcgcactgtt tttccagcgt cgcgccaata tgcagccccc
     2161 acttaccgct caaaccttcc tgcgataacg cgcaatttag cttcgcggaa tcgagaataa
     2221 agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc agcgtcgttc
     2281 tggaaagcac cgtcagcgga gactcttgct cgccctctac cacacacgcc tgctgggtaa
     2341 acaccacacc atcgtgcgtc tcaatatgca caatgttggt atgcccgccg acgatggtga
     2401 cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt tcattgcaag
     2461 gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca tcggcaattg
     2521 cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca cctaacgccc
     2581 ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg acgcccagac
     2641 cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga ccttccagtt
     2701 ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat cccagcgccg
     2761 gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt aaagtcgaat
     2821 caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg aacggagaaa
     2881 cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg tatttgtgca
     2941 atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg ccgaaaatcg
     3001 ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatggcg ctccaggcca
     3061 gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc ttctcggcag
     3121 gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga aagcctaaat
     3181 agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg accactttca
     3241 cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg gaaatattct
     3301 gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag cgtgaacgac acggcgtaga
     3361 aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat gctttatgcc
     3421 gcgccacttc aattgatttt tcccgcgagc gataagagat caccatcggg cttaacgtct
     3481 ggataaacag aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt ttcaccagca
     3541 gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc atcgacacgc
     3601 ccagcgccgc gaccaccagc agctttgtca gcaccatgcc agtcgaaatt ttgaataaca
     3661 atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga ccataaaagg
     3721 gactgtctga cagcaaccct tccgtcacgc cgaaggtatg caggtaggaa gcactatcgt
     3781 tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa tagagtgcac
     3841 ctaacaggat gccccagttt ttacctaaat aaccgctaat gacgctcggg taatctttac
     3901 attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac attgccgggt
     3961 aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc tgcaccggga
     4021 gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag ccggtgtcag
     4081 tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca cgacccgccg
     4141 gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat ttccataatt tctcgctcaa
     4201 tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt
     4261 tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga aaatcttcac
     4321 aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta atcgcaagag
     4381 aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag
     4441 gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat
     4501 gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta
     4561 tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct
     4621 ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga
     4681 tcgcaggtca gtccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc
     4741 ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc
     4801 tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata cagcgcgcca
     4861 atagccccgg ctgcaagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac
     4921 ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt
     4981 accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag
     5041 tcgatgacat tcatcggatc gttagagatg ttatcgctgg aaaccagttg acgacgcagc
     5101 gctacggcac ggcgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg
     5161 cgttcaatac cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct
     5221 ttactgcgca gcgctagctc gttgtgcatc atcaggccag aaatagacag gccgttgtaa
     5281 tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca
     5341 tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag
     5401 gttttactta atagctcttc ctgtgccttc caggcagtga tccgcattcc gttctcatgg
     5461 cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc
     5521 gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc
     5581 tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc
     5641 gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc
     5701 gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt
     5761 ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta
     5821 atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttacgaccg
     5881 tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc agcttcacat
     5941 ctttcggcaa acgcgcgacc tgtacacagc tccgggtcgg gtaggtcgcc tgatgctcat
     6001 cgaagaactg cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgatgaaca
     6061 cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt
     6121 tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc
     6181 aaaccggaat ttgaccggag gtgaagacca tgctgcctaa atcaacgcct tgaacataag
     6241 ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt
     6301 cagagcgcct gggtaaaggt acgtgaaata acatcctgct gctgttcacg ggtcagtgcg
     6361 ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc
     6421 tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct
     6481 tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt
     6541 actggatctt ctttgcccag cgccgcaggg acgattgaga aggtgtacga gatcccgtct
     6601 ttagcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcgg
     6661 tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc
     6721 ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg
     6781 gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa acgttcaacc
     6841 aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg
     6901 tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat
     6961 ttgatcgcag acagggagtc cgttgccacc gacaggcccg cgatgccgca tgccatggtg
     7021 cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc
     7081 atatagtgaa tgatattcag cgcactgatg tactgcaccg ccagccagtc catgaagtga
     7141 tcgaggctgt ccatcacttt gtcgtaatcc agcacatcgt ccatcaacgg tgctgttttc
     7201 ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta gagcagcgtt
     7261 ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg
     7321 acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcgttt
     7381 tcatattgca aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc
     7441 ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgcaggccc catagtgtgc
     7501 agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc cagccccatc
     7561 ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggt
     7621 gtacgcagga agcgcaccat acggatcttc atgatgaagt gatcgatcag ttcctgtgcc
     7681 tgctgctcat tgagtacgcc agctttaaag tcgcgctcaa tgtagatatc caggaacgat
     7741 gccgtgcgac ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc cagataagcg
     7801 aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca
     7861 tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca
     7921 cgcagacgga tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca
     7981 aattgcagtt cgcgttcacg caccagataa cgaatgccat acagcgctac gcggcgatag
     8041 tcaccgataa tgcgcccacg tccataacca tctggtaaac cggtcagtac gccagattta
     8101 cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc
     8161 agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc gtggaatgaa
     8221 cttttaatca tattaatgcc accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt
     8281 tgcaggccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatt
     8341 gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc gatacggatg
     8401 ccttccatca ccttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat
     8461 tcatcacctt catacggtgt atagttatgt tgaataaaat cgcggacatt aatttcgctt
     8521 ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca
     8581 atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct
     8641 gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta
     8701 gtcggaataa cggcgcaaat gacacgcgca ttttcactgg aaacaattcg ctcgccaaaa
     8761 gagttagatc gattattcat ttctgtatca atctctacgc ctaatacagc caaatgttcc
     8821 atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca
     8881 tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag
     8941 gttttaattg ccagctgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt
     9001 aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc
     9061 aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc aaagtcgaca
     9121 tcgccactgc gggtacccat catcaagcct tccagcggcg tcattcccat tgaggtatca
     9181 acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg cgccacaacc
     9241 aggccggagt catcttccgc cagattcagc agcgaatgtg cgcgctggga aacatagcgg
     9301 tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc
     9361 aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc
     9421 acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa
     9481 ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata
     9541 atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccacg
     9601 ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag
     9661 ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag
     9721 ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc gagtacggaa
     9781 aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct
     9841 tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag
     9901 accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc
     9961 tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg gtgcgcccat
    10021 ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccaag tggagcccat
    10081 gatgaagatc atgctgagag tattcagttt acccagcgac actttggttt tgtcaccttt
    10141 ataaccgaac ttcagaatca gaccattcaa gccttccagc gtccccagat agtgaccgaa
    10201 gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca gtgtaattgc
    10261 gaacgttgtt ttcgtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg
    10321 aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaaca ggcagctaaa
    10381 ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga tttgggaaca
    10441 tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt tagaaaccac
    10501 gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca
    10561 gacagtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt caacctggtc
    10621 gataactgca gagttccagt aagggatcag cgacaaagaa atcagcacca ggctggcgat
    10681 aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa accagatgac
    10741 gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc
    10801 gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacag
    10861 cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa
    10921 atgctcttcc accgtttccg taatgttgcc ggaaggatta gagccagaaa gacacagacg
    10981 cgccagcgcc cggtggcaat aaaacgcgat ggggtatgcc aataccaaca tcagaagaat
    11041 cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc
    11101 cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt
    11161 ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc
    11221 gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat
    11281 actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca
    11341 tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact
    11401 gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc
    11461 gtaagttaaa ttacccgggc gggcgacatc acaaccatcc gccagggtgc cggtagttcg
    11521 gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttctgactg
    11581 tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa taccagcaat
    11641 taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat cttccataat
    11701 ttccaggcca atcgttccct gaccagcaat cactttcgga tcatcgtaag gtgggataaa
    11761 aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt tgaagttatc
    11821 accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg attttggcgc
    11881 accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga gggaaacccc
    11941 ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg catcggtcag
    12001 tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct gcatattttc
    12061 aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatacc
    12121 tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatgtcat caatagcaac
    12181 cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa tatgtaggta
    12241 accgtaaccg acacctgcaa gacagacagg tggattattt atgtttaaat aaaaacaaat
    12301 aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga atactctttg
    12361 gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga ccataccgcg
    12421 gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg attagaacca
    12481 aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag aacaagatta
    12541 taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc atttctttgt
    12601 aacgtagtaa gcagttcgct gtagtacccc atattggttt gtggcaacac ccactgttcg
    12661 ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact ggctaccagc
    12721 acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat ttctgcactt
    12781 aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt
    12841 tgcgcttcat acatagaaac ctgcgctttt gggaacacct ctttgaactt attgatcatc
    12901 cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc caccaccgcc
    12961 tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt aatggattcg
    13021 gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt tttccgcacc
    13081 actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac ggctggttga
    13141 gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac ttcctgaaag
    13201 actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta cgttaattac
    13261 ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg accactcaca
    13321 aattaacttt caatactttc cagggtatcg tcatgtcaat taaatacctt taaatatcaa
    13381 taagttaaag tataaaaatc agcataaacc ctgatttttg tcaaaaaaaa tgacggggat
    13441 aaccatattt tatggcgata acatcattcg ttatgtggtt aatgcaaaaa agaggctgag
    13501 accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa atttaatgag
    13561 caagttctca aattttatta taaataaacc attttcagcg ataaataatg cggcacgtca
    13621 cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt ttaaaatttc
    13681 gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct cttctgacag
    13741 gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccttcat aggcgacgaa
    13801 tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga taaggtcaga
    13861 caaagacaac aagggaaatt ttcacagagc ttttgatcgg cgtaggccac agaatgttgc
    13921 ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag aaccctgcga
    13981 ggaaaataag catctattat tgtttgtcat gatgacaagg caggcatcaa tgaaaggctt
    14041 cccaattgca catatttttc acccttcaat cccaccaatg cacgcagtgg ttaacaatca
    14101 caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg tctccaccaa
    14161 tgacgttaat aaaatataca gtataagtaa tgaactgcgg agagtattat ctgcaattac
    14221 tgcattgaat ttctatcaag gcgatgttcc ttctgtcatg atccgaatcc aaccggaaaa
    14281 tatgagtcca ttcatcatag atatttctac aggagaacat gacgattata tcatacaaac
    14341 attagatgta ggcacttttg caccttttgg cgaaaaatgt acttgctcag ccgtcaataa
    14401 aaaagagctg gaatgtatta aggagacgat ttctaagtat tgtgcaaaat tcacccgaaa
    14461 agaagccatc ttaacacccc ctgcgtactt taataaaacc agcattactt cagattgttg
    14521 gcaaattctc tttttttcac ccgatcattt taataatgat ttttattgat tatttataaa
    14581 ggataaaata atgttccctg tttcatcaat tggtaatgat attagtagtg atttaatccg
    14641 tcgaaaaatg aatgaccttc ctgaaagccc aacaggaaat aacctcgaag cactagcacc
    14701 aggtatagaa aaactaaaac agacctctat tgaaatggtc actttactta acgcgttaca
    14761 accaggtggg aaatgtgtta tcactggtga ttttcaaaaa gaattagcgt acttacaaaa
    14821 tgtaattctt tataatgatt cgtctcttcg tatggatttt tttggttata acgcccgaat
    14881 tattcaacga tcagacaata cctgcgaact tacaattaat gaaccgttaa aaaaccagga
    14941 aatatccaca ggcaatatca atgttaattg tccattgaaa gatatttaca atgaaatcag
    15001 gaggttaaac gtagttttta gttgtggaac tggaggtata gttgatctat cctctctgga
    15061 cttacggaat gtcgatttag aactttatga ttttacagat aaacatatgg ccaatgcaat
    15121 tttaaatcct tttaaattgg atgacacaga ttttactaat gccaacatgt ttcaggtaaa
    15181 tttcgttagt tcaaaacaaa acaccacaat ctcctgggat tatttactaa aaataacgcc
    15241 tgttctaaca agcattagcg atatgtattc tgaagaaaaa attaagcttg tcgaaagttg
    15301 tttaaatgag cttggagata ttaccgaaga acaattaaaa atcatgagat ttgcaattat
    15361 agaatc
//
LOCUS       DAZGCQ010000109        15230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000109 DAZGCQ010000000
VERSION     DAZGCQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.109"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHK07_003983"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHK07_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO8104256.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHK07_003984"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHK07_003984"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO8104257.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHK07_003985"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHK07_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO8104258.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHK07_003986"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHK07_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO8104259.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHK07_003987"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHK07_003987"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO8104260.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHK07_003988"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHK07_003988"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO8104261.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHK07_003989"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHK07_003989"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO8104262.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHK07_003990"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHK07_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO8104263.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            8598..9272
                     /locus_tag="ACHK07_003991"
     CDS             8598..9272
                     /locus_tag="ACHK07_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104264.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            9395..10021
                     /locus_tag="ACHK07_003992"
     CDS             9395..10021
                     /locus_tag="ACHK07_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104265.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHK07_003993"
     CDS             complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHK07_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO8104266.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10731..11576
                     /gene="glpF"
                     /locus_tag="ACHK07_003994"
     CDS             10731..11576
                     /gene="glpF"
                     /locus_tag="ACHK07_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO8104267.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11599..13107
                     /gene="glpK"
                     /locus_tag="ACHK07_003995"
     CDS             11599..13107
                     /gene="glpK"
                     /locus_tag="ACHK07_003995"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO8104268.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13337..14347
                     /gene="glpX"
                     /locus_tag="ACHK07_003996"
     CDS             13337..14347
                     /gene="glpX"
                     /locus_tag="ACHK07_003996"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8104269.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14444..15190
                     /gene="fpr"
                     /locus_tag="ACHK07_003997"
     CDS             14444..15190
                     /gene="fpr"
                     /locus_tag="ACHK07_003997"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO8104270.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKESSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
BASE COUNT         3722 a         3822 c         4064 g         3622 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagctgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cgggattgtg gtatcagtta
      721 gcgatgccag cgaactgccg ctcaatgagc taaaagcggt agtcgaagtg ctggatagtg
      781 agccggtgtt tactcactcc gtctggcgat tgctgctatg ggcggcagat tattatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actacgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg gtggatctga
      961 acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg caaggcaaaa
     1021 tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagtgc ggcggatacc ttctctgcct ggctgctggc gggtgttaca ggttccggta
     1261 aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt tcggcgtggt
     1441 tgaaagcgaa aaatggtgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac
     1741 acgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac cgccgtggct
     1861 ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagccaac
     1981 gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatcttg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg caaacgttgc
     2401 tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcgacgc atgatgcagc
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaat
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg
     2701 cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat gttgatccga
     2761 ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 cgtctcccga gcattaatga atcccgataa agtctctcag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atagaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 tgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggaa
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgctggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaggtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggct
     4861 ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggttaaagcc gccgttgagc tggcaaaaga
     5341 ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 cgcttcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgc gcgctgttag aaaacactga
     5521 acttagcgcc cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca ctattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg
     7141 gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgca gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctct gggctggcac tggttgcagt
     7381 ggcatgccat acgctggctg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttcagaagat
     8521 ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat tccccttttt
     8581 tatacatgga cgtattcatg cagaatatta ttcttttatt tatatattta ttacttatag
     8641 ccgttaacca tttccgctac aaatttcttt tatctggcaa cgcgggtgaa atgattttat
     8701 attttgctaa cgtatttttt aacccacttg ccttatcatt tattatcgcc acaatcatct
     8761 gtataacgat aaaaaaaaga agcagccgca gctttattcg cggcacttgt tggttaatgg
     8821 gattatttct tatctatagc agctatacgg tgtgggaacg acacagcata tgggattata
     8881 ctttcccgga gccaggtatc accgtcacgg ttccgagcaa acagtgggtt gccaatatag
     8941 taaaacaagg accaaccctg gtgacccggg atgcccatgc tattttagct attgttgcct
     9001 tctcacaaaa tgaactcggt gtgcattcaa tcgaggaact taccgcgact caaaacggaa
     9061 ctgcagaaat gcatgtttgt gatgttcagg gatttgcctg cgcttatcag gaaggagtgc
     9121 aaacaatgag tgatggcaaa gtaagacatg ctattttcat gactctactg gataacacta
     9181 atttgataca actggtcgca gctatcgacc cagattattt ggacgaatat caagaagaag
     9241 taatgaaaat gatgctttct gctcgtcaat gatctgatgt gcatgttaat aataatggat
     9301 tgatatgaga tacgttatct tattaatact atggataata actgtttttc ttagtcatct
     9361 tcagcacagt aacttgctta ctggagatcc tgggatggtt ggcgagtata tcggttcagt
     9421 acttcttggg ccgttattac ttcccgtgct tgtgtcaggt attctgtgca cattcactaa
     9481 aaaaacgcgt aacttcgcca gttttactcg cgggtgctgt tgggtattgg gagtcctgct
     9541 tttatccaac gtcggcaata cgtttcgtct gttcaccccg tggcactata cgtttgagaa
     9601 ggcagctatt tccgtgactg tcccaaaccg acactggaat acggtttcta ttagtactga
     9661 taaaaccatt gatataagaa gtgaagataa tagcgtcttt atttctgctt tccgcctgcc
     9721 cgcaggacgt agcgccgatg actcgctgga agagttaaag aagatgcagc gagacaatct
     9781 caaggatcaa tacaatgaag aaaccttcca gtttcatgat tgtaatgcca aacatttcac
     9841 ctgtaagtat caggatgtgt tgataaattt tgatggtcag cagaaaagaa ccatcagtgt
     9901 ttatcttgaa gacaccccca gggctgtggg gatcattgca ttaatggagc cagatacggc
     9961 agataaatat cgccaacagg cgatggagat tatgttgtct gctaaaaata cagtgaagta
    10021 aaaaataagg cgccttgcgg cgcctctgcg tggaagagat taaacctctt ccatgcgacc
    10081 cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc
    10141 acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt
    10201 tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg
    10261 tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg
    10321 caaggcgttg atggataaaa aatcctcgtc ccgattaccg gtgacgcctt aataaatacg
    10381 agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca gcggcacgcc
    10441 ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat ccacaccaat
    10501 cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca tgtacaatca
    10561 gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat tttaagttcg
    10621 atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag catcgtggag
    10681 gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt atgagtcaaa
    10741 catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg ttgatttttt
    10801 tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa
    10861 tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca ggggtttccg
    10921 gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt ttcgacaagc
    10981 gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct gcggctttag
    11041 tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac attgttcgcg
    11101 gcagcgttga aagtgttgat ctggctggca ctttctctac ttaccctaat cctcatatca
    11161 attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg gggctgatcc
    11221 tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc ttgctgattg
    11281 gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacgggtttt gccatgaacc
    11341 cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc aatgtcgcct
    11401 ttaccggcgg cagagacatt ccttacttcc tggtgccgct ttttggccct atcgttggcg
    11461 cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct tgcgatatct
    11521 gtgttgtgga agaaaaggag accacaactc cttcagaaca aaaagcttcg ctgtaatatg
    11581 actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc
    11641 accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt gtcgcagcgc
    11701 gaatttgagc aaatctaccc aaaaccaggc tgggtagaac acgacccaat ggaaatctgg
    11761 gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa
    11821 attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc
    11881 ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag
    11941 catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct ggtgattgac
    12001 ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag
    12061 cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat ctggaaaatg
    12121 actcagggcc gtgtccatgt gaccgattat accaacgcct ctcgtaccat gttgttcaac
    12181 atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg
    12241 ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga ctaacattgg cggcaaaggc
    12301 ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag
    12361 ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt tatgctgatg
    12421 aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc
    12481 ccgactggcg aagtgaacta tgcgttggaa ggtgcagtgt ttatggcagg cgcatccatt
    12541 cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga atatttcgcc
    12601 accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg gctgggtgcg
    12661 ccatactggg acccgtatgc gcgcggggcg attttcggct taacgcgtgg agtgaacgct
    12721 aaccacatta tccgcgcgac gctggagtct attgcttatc agacgcgtga cgtactggaa
    12781 gcaatgcagg ccgactccgg tattcgtctg cacgccctgc gcgtggatgg cggcgcagta
    12841 gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt tgagcgcccg
    12901 gaagttcgcg aagtgactgc attgggtgcg gcctatcttg ctggtctggc ggtcggtttc
    12961 tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc
    13021 atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg
    13081 atggcgtggg aagaacacga cgagtaatgt caggcacctg aatcgattgc ctgatgctga
    13141 tcgcctgatg cgacgcttac gcgtcttatc aggcctacga gccgatgcca ttttgtgtag
    13201 gcgggataag gcgttcacgc cgcatccggc atcacgcaga ctctgaaacc gtgcgggggc
    13261 aaccccgcac acatcaataa tccctccctt cccctgtgct acacttcgcg ccattcctta
    13321 ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc
    13381 agcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg cggacggcgc
    13441 ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca ccatcgtcat
    13501 tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag tcggtactgg
    13561 tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca tgacggcgat
    13621 gggtcaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgtt tcctcaacgc
    13681 gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca ccattgatct
    13741 gaacctgccg ctggcggata acctgcgcaa tgttgcggcg gcgctcggca aaccgttgag
    13801 cgaactgacg gtaacgattc tggctaaacc acgccacgat gccgttatcg ctgaaatgca
    13861 gcaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct caattctcac
    13921 ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt
    13981 ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg
    14041 tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg
    14101 ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ctgggcgata tggcgcgcag
    14161 cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg aaggcattag
    14221 ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat
    14281 tcgccgcatt cagtccatcc actatctgga tcgcaaagac ccggaaatgc aggtgcacat
    14341 cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat ttttttgttc
    14401 ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg attgggtaac
    14461 aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca ccgttcacgc
    14521 tcccgtgctt ccgtttaccg ccgggcaatt taccaagctt ggccttgaaa tcgacggcga
    14581 acgcgtccag cgcgcctact cctatgttaa ctcgcccgat aatcccgatc tggagtttta
    14641 cctggtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga aaccgggcga
    14701 tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag taccagattg
    14761 cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat cgattctaca
    14821 actaggcaaa gatttagatc gcttcaaaaa tctggtcctg gtgcacgccg cacgttatgc
    14881 cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg aaggaaaact
    14941 gcgcattcag acggtggtca gtcgggaaac ggcagcgggt tcgctcaccg gacggatacc
    15001 ggcgttaatt gaaagtggtg aactggaaag cgcggttggc ctgccgatga ataaagaaag
    15061 cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacgcaac agttgctgaa
    15121 agagacccgg cagatgacga aacatttacg tcgccgaccg ggtcacatga cagcggagca
    15181 ttactggtaa gcggttactt atcgataaac ggcacgatca agagatccgc
//
LOCUS       DAZGCQ010000110        15176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000110 DAZGCQ010000000
VERSION     DAZGCQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.110"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1575)
                     /locus_tag="ACHK07_003998"
     CDS             complement(<1..1575)
                     /locus_tag="ACHK07_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8104271.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNEISDKFNIVLEITERNLVEDTDLALQKINELRIHGCEFAVDDFGTGYCSLGL
                     LQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTPHQAEYLI
                     HNNVTLVQGYLYAKPMK"
     gene            complement(1673..3031)
                     /gene="dgcT"
                     /locus_tag="ACHK07_003999"
     CDS             complement(1673..3031)
                     /gene="dgcT"
                     /locus_tag="ACHK07_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8104272.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQMIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVLG
                     GPIVAHILSSHYESYDLHIAELTNENGQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMLMDIDHFKKVNDTWGHPIG
                     DQVIKTVVNIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            3620..6043
                     /gene="pgaA"
                     /locus_tag="ACHK07_004000"
     CDS             3620..6043
                     /gene="pgaA"
                     /locus_tag="ACHK07_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO8104273.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQTRGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            6052..8070
                     /gene="pgaB"
                     /locus_tag="ACHK07_004001"
     CDS             6052..8070
                     /gene="pgaB"
                     /locus_tag="ACHK07_004001"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO8104274.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFRWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLIEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLINQIKSIPQAKDKSILELQAKNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            8063..9388
                     /gene="pgaC"
                     /locus_tag="ACHK07_004002"
     CDS             8063..9388
                     /gene="pgaC"
                     /locus_tag="ACHK07_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO8104275.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            9390..9803
                     /gene="pgaD"
                     /locus_tag="ACHK07_004003"
     CDS             9390..9803
                     /gene="pgaD"
                     /locus_tag="ACHK07_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO8104276.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(9853..10641)
                     /gene="phoH"
                     /locus_tag="ACHK07_004004"
     CDS             complement(9853..10641)
                     /gene="phoH"
                     /locus_tag="ACHK07_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO8104277.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(11273..12544)
                     /gene="efeB"
                     /locus_tag="ACHK07_004005"
     CDS             complement(11273..12544)
                     /gene="efeB"
                     /locus_tag="ACHK07_004005"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO8104278.1"
                     /translation="MQYEDENGVNELSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLVKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(12550..13677)
                     /gene="efeO"
                     /locus_tag="ACHK07_004006"
     CDS             complement(12550..13677)
                     /gene="efeO"
                     /locus_tag="ACHK07_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO8104279.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTEADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(13735..14565)
                     /gene="efeU"
                     /locus_tag="ACHK07_004007"
     CDS             complement(13735..14565)
                     /gene="efeU"
                     /locus_tag="ACHK07_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO8104280.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         4245 a         3581 c         3317 g         4033 t
ORIGIN      
        1 tttcattggt tttgcatata agtatccctg aactaatgtc acgttattat gaataagata
       61 ttccgcctgg tgaggagtac tgacaccttc tgcaatcgtt atcaaattga gtcgattact
      121 cagcttaata ataatatcta aaactggcgt ttcgtcttcc gctgtagtga gtgtgtctat
      181 aaagctttta tctattttaa gataatcaac agacaacttt tgcagaaggc caagggaaca
      241 atatcccgtg ccaaaatcat caactgcaaa ttcacagcca tggatcctta attcatttat
      301 tttctgtaat gcaagatcag tatcttcaac cagattgcgt tcggtaatct caagaacaat
      361 gttaaattta tcagatattt catttttaat atgtaaaaca tcagcaacaa aatcaggatg
      421 tgcaagatgc tccgcggcaa tatttatccc aaggtgaaac ggctttttca ctttccagct
      481 ttgtacatcc tgaataatca gttcaaagag atgcttagtt aacggtataa ttaaattttc
      541 ctcttctgca gcgcggataa aaactaccgg agatatattt ttaccatctt cgcgttgcca
      601 acgcattaac gcctcaaccc ccagacacgc tttggttgtc gtgtcacata ctggttgata
      661 atgcacagaa aattctccgg atgacattcc tttcttaatt tcttctgcca gggacatttt
      721 ccgagatatc cagcgctgcc ataagaagta caggataaaa gaaagaagaa tagataatgg
      781 aagaatgtaa aatacattac taatgacata gggatataat gaaatcatcg gcgtagtaac
      841 tgtaagacta gcctgactga tcgcagaatt aaaatctttt tttaacatat ataacctgtc
      901 attattattt acaccactaa taattgcagg cccttcagta aagcgaagag aaaatgaatc
      961 atcattctca tcatcaagaa catccatcag atcatagata tatcgtgact ctacaatact
     1021 aaaagctgtc atatcattcc ctattggcat aaggaacact atggctgttt tacctggtac
     1081 aaaacttgta ccgctaattg atactatgcg tgattcttta tttttattgt gaaagctcgt
     1141 accgtaaaca tcgttaacat tcattttttt gaaaccgcta actgaagaac atatttggaa
     1201 attattttct ataagacccg ttgttcgaac atagggggtc aatgctccgt tagcaatcaa
     1261 ttgttccagg atatctttgc agggtttcat caccagaata gaagtaagct ttgtcgtatg
     1321 tttgacaatt tgcgtaacat tatcaagctg ttttaacaga ataccggcgg taattgtcgt
     1381 atctcgattc atttgaaata ctgacatagc aaacgacagt aacaccccca gaatgcctgg
     1441 tattataatg agtacaaaca tttttttgtt gaataccata catgtttccc cacgcatcag
     1501 ggtgatgcaa cctaagaaat tatagtacaa ttgccagtaa aagcgggtga tagcagactg
     1561 atcaatgata agcatttaag atcatatagt actatgatat ttctgaaaat tgttacgcat
     1621 gtctgcgaat agctatttag cgcttaccaa aatcacccgc taaaatgccg ctttatggtg
     1681 actcacaaaa attcaccaca tccctcacaa ccaccttatt acgcccggtc tctttcgcct
     1741 cgtatagtgc gttatcagcc agtcgataga tctcattcgg gtttaacgca tttccctgag
     1801 tcacaacagc gccaatacta atcgtcactt tttgtggtat ggcgtattca ggattatcac
     1861 cagttaaacg ctcaacattt tcccgaatcc tttccgctaa agctttcgca cgttcagtat
     1921 cgatgtccgt cagcaagacg ccaaactctt cgccgccgac gcgcgcaaaa aggtcatctg
     1981 gccgtatgct tttcccaatg atattcacca ctgtttttat cacctgatcg ccaatcggat
     2041 gcccccaggt gtcgttgact tttttgaagt ggtcgatatc cataagcatg acgcaataag
     2101 gcgttttttt ggccgatgct gattgaaccg tcagttcatt aaaaaaataa tttctgttaa
     2161 atatattggt taagggatcg cgatgtgcaa tgtcctttgt tactcgtagc gcactgaata
     2221 tatggcacat aaatattacc ataacggtta acttactgac tacttcaata gtgcgactaa
     2281 tataccaaat ggacacgcta tacctgctca taaataaaag agagacattg tacagtacgg
     2341 cacaaaaagc aatcaccgtc actccattcc agatatcata gcgtaaacca ttaaagtata
     2401 gattaaccga aagcaaggtt aaccacatga aaatcattat ggtaacatat gaagttttcc
     2461 aaaccacttg accattttca ttggttaact cggcaatgtg taaatcgtag ctctcataat
     2521 ggctagaaag tatgtgagca actatcggcc ccccaagaac aaaaaacaaa cttatgcaca
     2581 aagtcatttt tttggaaaat aaattgcgct gttttgtgtt ttcactaact ttattcacca
     2641 atgccagaaa tattaatatg cacaaactca tctgacgaaa caaataataa attgcaatgt
     2701 cattttgaac aacactgctt gtcaaacgcc cctcaatcat ttgctgaatg ataatgacgg
     2761 ttttaacaaa gtaaatcaaa caactcagaa aagccaaact aagtatacag tttgaaaatt
     2821 gcctacggtc ataaatgaaa tacaactgca taaaaataaa tgcaacagta tcgataaaaa
     2881 ataaagcaat cattaccatc agataggttg atcgtggaac aacttcctca acgcctggct
     2941 gattaaacca actattcacc aaaaccaaag caagcccaaa caataagctc actaagacag
     3001 tactactaat tctcaaatag tctttttcca taaagctaca ccaacctgta ggttgtagat
     3061 ccctttctat tattaataga tataagaact gtacagttgt caatatgaaa acccactcac
     3121 aaacaatacc gatgatgata gttgcagacc gcaattatta ttaccttagt gctttataca
     3181 ttctttgcga caagacaggc ttgcaaatat ttataaatgg ttataaggaa atccttataa
     3241 aacattcatt catagatata ttaggaataa caattgaatc cgtgagtaca gtaacgctgc
     3301 atttcatcag gactttcgaa aaatccgaaa tcatgcctcg gaatttactg atttaattat
     3361 tttactccta atttattttg aaaaaggcat tgggatttat gccgtatttc tgaagatcct
     3421 catcattgga atggattttc gggcgagaaa aggattttat atggacactc tgctcatcat
     3481 ttcttcttct catcatcaac aattcacgtc tctcttccgc gtttaataac ggattatgag
     3541 gtgcaaaaaa tatctttctt ttcagttgcc tgtcattaga tacagagaga gattttggca
     3601 atacatggag taatacagga tgtattcaag tagcagaaaa aggtgcccgc aaaccaaatg
     3661 ggctttgaaa attcttactg ccgcattttt agcagcgagt cccgcggcga agagtgccgt
     3721 taataacgcc tatgatgcat tgattattga agctcgcaag ggaaatactc agccagcttt
     3781 gttatggttt gcacaaaaat cagcactcag taataaccaa attgctgact ggttacagat
     3841 tgccttatgg gccgggcaag ataaacaggt tattaccgtt tacaaccgct accgttatca
     3901 gcaattacca gcgcgtggtt atgcagctgt cgccgtcgct tatcgtaacc tgcaacaatg
     3961 gcaaaactcg cttacactgt ggcaaaaggc gctctctctg gagccgcaaa ataaggatta
     4021 tcaacgggga caaattttaa ccctggcaga tgcgggtcac tatgatactg cgctggttaa
     4081 acttaagcag cttaactctg gagcaccgga caaagccaat ttactcgcag aagcctatat
     4141 ctataaactg gcagggcgtc atcaggatga attacgggcg atgacagagt cattacctga
     4201 aaatgcctct acgcaacaat atcccacaga atacgtgcag gcattacgta ataatcaact
     4261 tgctgccgcg attgacgatg ccaatttaac gccagatatt cgcgctgata ttcatgccga
     4321 actggtcaga ctgtcgttta tgcctacgcg cagtgaaagt gaacgttatg ccattgccga
     4381 tcgcgccctc gcccaatacg ctgcattaga aattctgtgg cacgataacc cagaccgcac
     4441 tgcccagtac cagcgtattc aggttgatca tcttggcgcg ttattaactc gcgatcgtta
     4501 taaagacgtt atttctcact atcagcgttt aaaaaagacg gggcaaatta ttccgccctg
     4561 ggcgcaatat tgggttgcgt cggcttatct caaagaacat cagccaaaaa aagcacagtc
     4621 aataatgaca gagctctttt atcacaagga gaccattgcc ccggatttat ccgatgaaga
     4681 acttgcggat ctcttttaca gccacctgga gagtgaaaat tatcccggcg cgctaactgt
     4741 cacccaacat accattaata cttcgccgcc tttccttcgg ttaatgggca cgcctacgag
     4801 catcccgaat gatacctggt tacaggggca ttcgtttctc tcaaccgtag caaaatatag
     4861 taatgatctt cctcaggctg aaatgacagc cagagagctt gcttataacg caccaggaaa
     4921 tcagggactg cgcattgatt acgcgagtgt gttacaaacc cgcggttggc ctcgtgcagc
     4981 agaaaatgaa ttaaaaaaag cagaagtgat cgagccacgt aatattaatc tggaggttga
     5041 acaagcctgg acagcattaa cgttacaaga atggcagcag gcagctgtct taacgcacga
     5101 tgttgtcgaa cgtgaaccgc aagatcccgg cgttgtacgt ttaaaacgtg cggttgatgt
     5161 acataatctt gcagagcttc gtatcgctgg ctcaacagga attgatgccg aaggcccgga
     5221 tagtggtaaa catgatgtcg acttaaccac catcgtttat tcaccaccgc taaatgataa
     5281 ctggcgcggt tttgctggat tcggttatgc cgatggacaa tttagcgaag gaaaagggat
     5341 tgttcgcgac tggcttgcgg gtgttgagtg gcggtcacgt aatatctggc tcgaggcgga
     5401 gtacgctgaa cgctttttca atcatgagca taaacccggc gcgcgcctgt ctggctggta
     5461 tgattttaat gataattggc gtattggttc gcaactggaa cgcctctctc accgcgttcc
     5521 attacgggca atgaaaaatg gtgttacagg caacagtgct caggcttatg ttcgctggta
     5581 tcaaaatgag cggcgtaagt acggtgtctc ctgggctttc actgattttt ccgacagtaa
     5641 ccagcgtcat gaagtctcaa ttgagggtca ggaacgcatc tggtcttcac catatttgat
     5701 tgtcgatttc ctacccagtc tgtattacga acaaaataca gaacacgata ccccatacta
     5761 caaccctata aaaacgttcg atattgttcc ggcatttgag gcaagtcatt tgttatggcg
     5821 aagctatgaa aatagctggg agcaaatctt cagcgcaggt gttggtgcct cctggcaaaa
     5881 acattatggc acggatgtcg tcacccaact cggctacggg caacgcatta gctggaatga
     5941 cgtgattgat gctggcgcaa cgctacgctg ggaaaaacga ccttatgacg gtgacagaga
     6001 acacaactta tacgttgaat tcgatatgac attcagattt taaggataaa tatgttacgt
     6061 aatggaaata aatatctcct gatgctggtg agtataatta tgctcactgc gtgcattagc
     6121 cagtcaaaaa catcatttat accgccacag gatcgcgagt ctttactcgc cgagcaaccg
     6181 tggccgcata atggttttgt ggcgatctca tggcataacg ttgaagacga agctgccgac
     6241 cagcgtttta tgtcagtgcg gacatcagca ctgcgtgaac aatttgcctg gctgcgcgag
     6301 aacggttatc aaccggtcag tattgctcaa attcgtgaag cacatcgagg aggaaaaccg
     6361 ctaccggaaa aagcagtagt gctgactttt gatgacggct accagagttt ttatacccgc
     6421 gtcttcccaa ttcttcaggc cttccggtgg ccagctgtat gggcccccgt cggcagttgg
     6481 gtcgatacgc cagcggatga acaagtaaaa tttggcgatg agatggtcga tcgagaatat
     6541 tttgccacgt ggcaacaagt gcgagaagtt gcgcgttccc cgctcattga ggtcgcttct
     6601 catacatgga attctcacta cggtattcag gctaatgcca ccggcagctt attgcctgta
     6661 tatgtaaatc gtgcatattt tactgaccac gcacggtatg aaaccgcagc agaataccgg
     6721 gaaagaattc gtctggatgc tgtaaaaatg acggaatacc tgcgtacaaa ggctaaggta
     6781 aatccacacg tttttgtttg gccttatggc gaagcgaatg gcatagcgat agaggaatta
     6841 aaaaaactcg gttatgacat gttcttcacc cttgaatcag gtttggcaaa tgcgtcgcaa
     6901 ttggattcca ttccgcgagt attaatcgcc aataatccct cattaaaaga gtttgcccag
     6961 aaaattatta ccgtacagga aaaatcacca caacggataa tgcatatcga tcttgattac
     7021 gtttatgacg aaaaccgcca gcaaatggat cgcaatattg atgtgctaat tcagcgggtg
     7081 aaagatatgc aaatatcaac cgtgtatttg caggcatttg ctgatcccga tggtgatggg
     7141 ctggtcaaag aggtctggtt tccaaatcgt ttgctaccaa tgaaagcaga tatttttagt
     7201 cgggttgcct ggcaattacg tacccgcgca ggtgtaaaca tctatgcgtg gatgccggta
     7261 ttaagctggg atttagatcc cacattaacg cgagtaaaat acttaccaac tggggagaaa
     7321 aaagcacaaa ttcatcctga acaatatcgc cgtctctcgc cttttgatga cagggtcaga
     7381 gcacaagttg gcatgttata tgaagatctt gccggacatg ctgcttttga tggcctattg
     7441 ttccacgatg atgctttgct ttcagattat gaagatgcca gtgcaccggc tatcgcggct
     7501 tatcagcaag caggctttag cgggagtctg agcgaaattc gacaaaaccc ggagcaattt
     7561 aaacagtgga cccgctttaa aagtcgtgcg ttaactgact tcactttaga acttagtgcg
     7621 cgcgtaaaag ccattcgtgg tccacatgtt aaaactgcac gaaatatttt cgcacttccg
     7681 gtaacacaac ctgaaagtga agcctggttt gcacagaatt atgctgattt cctaaaaagc
     7741 tatgactgga ctgctattat ggctatgcct tatatggaag gtgtcgcaga aaaatcggct
     7801 gatcaatggt taatacaact gatcaatcag attaaaagca tccctcaggc taaagacaaa
     7861 tctattttag aattacaggc aaaaaactgg cagaaaaatg gtcagcatca ggctatttct
     7921 tcgcaacaac tcgctcactg gatgagccta ttacaactga atggagtgaa aaactatggt
     7981 tattatcccg acaattttct gcataaccaa cctgaaatag acgttattcg tcctgagttt
     8041 tcaacagcct ggtatccgaa aaatgattaa ccgcatcgta tcgtttttta tattatgtct
     8101 ggtgttatgc atccccctat gcgtagcgta ctttcactct ggtgaactga tgatgaggtt
     8161 cgttttcttc tggccgtttt ttatgtccat catgtggatt gttggcggcg tctatttctg
     8221 ggtctatcgt gaacgccact ggccgtgggg ggaaaacgca ccagctcccc agttgaaaga
     8281 taatccgtct atctccatta tcattccctg ttttaatgag gagaaaaacg ttgaggaaac
     8341 catacacgcc gctttagcac agcgttatga gaacattgaa gttattgccg taaatgacgg
     8401 ttcaacagat aaaacccgtg ccatcctgga tcgcatggct gcacaaattc cccatttgcg
     8461 ggtcattcat ctggcgcaaa accaggggaa agccattgcg cttaaaaccg gagctgccgc
     8521 ggcgaaaagt gaatatctgg tgtgcattga tggcgacgcg ttattagacc gcgatgcggc
     8581 ggcatatatt gtggaaccga tgttgtacaa cccgcgtgtg ggtgccgtaa ccggtaatcc
     8641 tcgtattcgc acacgttcta ccctggtggg taaaattcag gttggcgagt attcctcaat
     8701 tattggtttg atcaagcgaa cccagcgtat ctatggaaac gtatttaccg tttccggtgt
     8761 tattgccgca tttcgtcgca gcgccctggc agaagtgggt tactggagtg acgatatgat
     8821 caccgaagat attgatatta gctggaagct gcagttgaat cagtggacga ttttttacga
     8881 gccacgggcg ctgtgctgga tattaatgcc tgaaacgtta aaagggctgt ggaaacagcg
     8941 cctgcgctgg gctcagggcg gtgcagaagt attcctcaaa aatatgacaa ggttgtggcg
     9001 caaagaaaac tttcgaatgt ggccactgtt ttttgaatac tgcctgacga caatatgggc
     9061 ctttacctgc ctggtcggtt tcattattta cgcagtccaa cttgccggtg taccgttaaa
     9121 tattgaattg acacatatcg ctgcgacaca tactgccgga atattattgt gtacgttatg
     9181 tttactgcaa tttattgtca gcctgatgat tgagaatcgc tatgagcata atctgacttc
     9241 atcgcttttc tggattattt ggttcccggt tattttctgg atgctgagcc tggcaacgac
     9301 attggtatca tttacacgag tcatgttgat gcctaaaaag caacgcgccc gttgggtaag
     9361 tcccgatcgc gggattctga gaggttaata tgaacaattt aattattacg acccgacaat
     9421 cgccagtacg tttactggtt gattatgttg ccacaaccat cttgtggaca ttatttgctt
     9481 tgttcatatt cttattcgcc atggatctgc tgacgggtta ttactggcaa agcgaggcca
     9541 gaagccgact tcagttctat tttttgctgg cagtggcgaa tgccgtcgtg ttaattgtct
     9601 gggcgctgta caataagctg cgttttcaaa aacagcagca tcatgcagcc taccaatata
     9661 cgccgcaaga atatgcagag agcttagcaa tacctgatga gctctatcag caactacaaa
     9721 aaagccacag gatgagcgta cacttcacca gccaggggca aataaaaatg gtggtttcag
     9781 aaaaagcgct agtccgggca taaacaccca aaacaaagcc cggttcgccc gggctctgca
     9841 ccgataatac acttaactgt atgcatgcag cgtacgttgg caaagtgccg aacgtacgca
     9901 atcctcttta ccgaaccgga cgatcccgac catttcatct tcttcgaaac gttccagcgc
     9961 gtcacttaat ccggagcaca cgccgcgagg caaatcgcat tgcgtgatat caccgttgac
    10021 gataaccgtc acgttctccc cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac
    10081 attctgcgcc tcgtcaagaa tgacgactgc attttcaaag gtacgtccac gcatataggc
    10141 gaacggcgca atttccacct tccctatttc cggtcgcagg cagtactgca taaaggaagc
    10201 ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc
    10261 gatatctcca ggtaagaagc caagatcttc atcggcttgc agaactggac gggtgacgat
    10321 aatcctgtcg acatccttat gtatcagggc ctctgccgct tttgctgcgc tgatccaggt
    10381 ttttccgcac ccggcttcgc ccgtggcgaa tatcagctgc ttactctcaa tagccttcag
    10441 atagtgcaat tgcgcttcat ttcgcgcgag gatgggcgag gtatcgcgac tgtcgcgggc
    10501 cataccaatg gcttctacgc cgctcatctg cacaagcgag gtgaccgatt cttcttcacg
    10561 ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac gagctttgat
    10621 cactgctttt tgtcttccca tggagagcac cttgagttgt ttgtattcat cacacgcgcc
    10681 gttggcagcg cgattatgcg cacgaacatc agagggttgg cttccttgta agccatagtt
    10741 tgcttttgga taaaatgccg aaaaacggct acgcgcaccg tttacggcgt cggtaacaca
    10801 tgaaaagaaa ggatgaggtt gaaaatgcaa agtgacgaga tgactaccgg aggagaaaac
    10861 tccgcgagtg gtggcgcgtt gattatctaa aacatgtcca gtacaggacg ttaccatccg
    10921 cgatctccat agtgactgac tatcactgcc gggaacttcc gctgctactt aataagtaca
    10981 acagatctcg cattcattgc aacaatatat tcacttatat ttaactataa aacaccattt
    11041 tagtgacatt agtttctact ggaaagatga cagagtgatg acagagtgat gacagagatg
    11101 aaaaaagctg tgtgctttca gcaggattca catcaggcat cagtgcacga acgtcggatg
    11161 cggcgtgaac gccttatcca gcctaccaaa cccgcaccga ttgtgggcaa cataagcatc
    11221 gcaagcgccg catcaggcat ctgcgcacaa atgccttatc cgcctaaaaa cattaaaccc
    11281 gcaataatgc gcttccgaga taatcgttcg cgtccttcac tcccggcagc gcaaaaaaat
    11341 aaccgccgcc gataggttta acgtattcct ccagcgcttc gccattgagc cttttttgta
    11401 ctgtcaggaa gccttttacc agatcgtgct ggtagcagac aaacagcaac cccatatcca
    11461 gttgtccgga gttggtgacg cccagtgaat aactgtagcc acgacgcagc atcaggctgg
    11521 actcactctc cgccgtgcgg ggattcgcca gccggatatg gctgtccagc gcgatcacct
    11581 tcccttccgg gtcgctggcg taatcaggca catcatgctc atgctgcatt cccagcggcg
    11641 caccggtttg cttatcacgg ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc
    11701 aaaattccac tcgaaactga atcaagcgta ctgcctgata gctgccaccg attgtccacg
    11761 caggctcctg ctgatctgcc gttacccaca ccactttttg catcaactta tcattctggc
    11821 tatcgggatt ggcagtgccg tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt
    11881 tactacgcgc cgcgtgatcg gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat
    11941 ccggcgtgtg tttgatgata tcgcgcagcg catggatcac cgtgtcctgg gtgttggcgc
    12001 aaatttgtag caacacatca ccatggcata acgccgcatc cagcgagtcg ttggggaaac
    12061 gcgtcatctt ctgcaacttt ttcggcatct gtggcgcaag gccgaagcgc tcatcaaaca
    12121 atgagtgacc caccgataac gtgatggtga gattatcggg cgcaatgtag ccgccaagaa
    12181 tgccggaatc gagtggtggc aggcgcggat ttggcgtttc tggtgctgct ccgccctgag
    12241 tcagaaaagc aaaacgctga gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc
    12301 tggcaagcac atcaaacgcc accagcatca ttgcggcctg ttgtggcgtc aggatccctg
    12361 cctgatgctc accataaaac ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg
    12421 gtgcactttg cgttttttgt gcatgagcga ccggacaact tcccgccagc gccagcgcac
    12481 caattccttt cagtaaacgt cggcgtgaca gttcattcac gccgttttca tcttcatact
    12541 gcataacgct taatccagtc ccagcacacc acgaagttgc gccagatctt ccgccagcgc
    12601 agtaatcggc cctttcagcg cgttccggtc ggcctcggtc aatttgtcgt aggtttcaaa
    12661 accgtcttta gtacggtatt tcgccagaat ggtatcgacc tttttaaagt tggcatcgac
    12721 ttttgccagc agttccgggt tggctttttg cagttgtgga cgcagcagat cgacaatttt
    12781 ctgcgagcct tcaacgttag cctggaaatc ccacagatcg gtgtggctgt agcgatcttc
    12841 ttcaccgctg attttgctgg ccgccacttc ctcaatcagt ccggctgcac cgccaaccac
    12901 ttttgaaggt gggaaagcca gttcactgat gcgtttttgt aaatcgacca catcggtata
    12961 aagctgctca gcgtactgat ccattccttt ggtggtgttg tcgccaaaca atgctttttc
    13021 cagacggtgg aaaccggtga attttggatc agcggctttt tgctcgtaat catcttcacg
    13081 ggcgtcaatg ctgccatcca gatcggagaa cagttcagca atcggctcaa tgcgctcata
    13141 gtgctggcgc gtcggcgcat acagtgcttt cgctttttcg atatcgcctg ctttaatcgc
    13201 gtcggtaaag gctttggtgt cggtcaccag ctgtgtggtt tccgccatga catacgcttt
    13261 atatgcagta attgcaccac caagacttaa cagcgcatca ctttgcgccg catccgccgt
    13321 tgcctcacct ttgacgatca acttcccttt cgggttagtc agcagaccgc aggtcatatc
    13381 gtattcgcca ggctgtaaat tcgccgtcat tttctggcta aaaccagggg cgatattttc
    13441 ccgctcttcc accaccatca cgcctttgag gatctcccac tccagcgcct tctggctgtg
    13501 gttctgaata atgaactgtg ttttcccggc gttaaccgta atggtcatcg gttcgcactg
    13561 tttatccgtc acggtcactt tgacttgcgg aatatcagcg gcgttagcca taaaagcaga
    13621 agagaacagc gcagccacgc tcaactgcaa tgcgttacgg cggaagttaa tggtcatgac
    13681 gagactatcc ctttaaagta tgttgtaact aagcaagagt ttgcgtcgta tttgctacgc
    13741 ggagcgagac gctgtcgccc ctgcgcgtgg tggcagagca aatgccacca gcgccgggat
    13801 gagataaata aaccagacgg cgacttcgct gacgctcggc gcttcctgat aaccaaaaat
    13861 cccttccatc aacgtgccaa acagcgagtg agttgagagc accgcgctca tatcgaaggc
    13921 gatttcctga aagtggttcc acaatccggc ttcatgaaat gcgcgaatgg cacctgcagc
    13981 cagccctgcg gcgacgaaga gaataaacag gctggtccat ttaaaaaatg caccaagatt
    14041 gaggcgaata ccgccccagt agagcaggaa gccgagcacc acggcagtag caagaccgag
    14101 cattgcaccc agcggcggcc agatcccgac atcttgttga aatgccgcca gcaggaaaaa
    14161 gaccgactcc aggccttccc ttgcaacggc aaaaaagacc atcatcacca gcgcccagcc
    14221 atgatgattt ccacgctgca atgcgctatc gactgcctgt tccagttgca ctttgacgtt
    14281 gcgcgacact ttgcgcatcc agaaaaccat ccaggtaagg atcaccacgg cgatcaccgc
    14341 cacgatacct tcaaacagtt cctgctcttt ttgcggaaat tcgccggtgg tttcgttaat
    14401 gaagataccc aagcccaggc acaacgcagc ggcaagcaac acgccaatcc acatcacacc
    14461 aatccatcgg cctcgctggg tacgcttaag atagctggca atcaaactga caatcagcgc
    14521 ggcttcaagc ccttcgcgca acataatgag aaatggaaca aacatgccac ccacccttaa
    14581 acgtttttct gagtcaatct atgcaaagaa aagtaaatcg cagtgatagc gattatcatt
    14641 acgctaaaga aaaatacaag cggaatgttt ataaaatgat gttggaatgt aaaaatgagt
    14701 agatcaaatc cggagaaaaa agcgattatt ttaaggtatt tagctgacgt ttcgcccggc
    14761 agatgaagct tgtacgaatg ttggtggcga caaactcgct gcagcgtgcc ccgaagggtt
    14821 gaattttcag ttagtcgtat cacaaaagaa cgttgttttt tgcttattaa tagatagtga
    14881 tagggttgtg aatgaaatta tacctcgtct cctcatcgaa tttttcgccc acaacctaaa
    14941 caggagaaaa cgtgtattta agtctggaat gagcgatttt tgatagtgcg taattaaccc
    15001 aaaaaattat aatccccctt attatatttc cagtgatatc attttatggc taaagctata
    15061 tacaactcta aatcacgata aaacgaagca ggatgcgcat taaaaaagtc acacataata
    15121 aaaatagaaa tcttaaccag aagtaataga tcatcgtgaa agtcaatcat caaaaa
//
LOCUS       DAZGCQ010000111        14937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000111 DAZGCQ010000000
VERSION     DAZGCQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.111"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            234..776
                     /gene="yfaZ"
                     /locus_tag="ACHK07_004008"
     CDS             234..776
                     /gene="yfaZ"
                     /locus_tag="ACHK07_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO8104281.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQVGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            876..2078
                     /gene="yfaY"
                     /locus_tag="ACHK07_004009"
     CDS             876..2078
                     /gene="yfaY"
                     /locus_tag="ACHK07_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO8104282.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2298..3080
                     /gene="yfaX"
                     /locus_tag="ACHK07_004010"
     CDS             2298..3080
                     /gene="yfaX"
                     /locus_tag="ACHK07_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO8104283.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3095..4300
                     /gene="rhmD"
                     /locus_tag="ACHK07_004011"
     CDS             3095..4300
                     /gene="rhmD"
                     /locus_tag="ACHK07_004011"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO8104284.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4357..5646
                     /gene="yfaV"
                     /locus_tag="ACHK07_004012"
     CDS             4357..5646
                     /gene="yfaV"
                     /locus_tag="ACHK07_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104285.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5664..6467
                     /gene="yfaU"
                     /locus_tag="ACHK07_004013"
     CDS             5664..6467
                     /gene="yfaU"
                     /locus_tag="ACHK07_004013"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO8104286.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6508..6741)
                     /locus_tag="ACHK07_004014"
                     /pseudo
     CDS             complement(6508..6741)
                     /locus_tag="ACHK07_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6742..7713)
                     /gene="rpnE"
                     /locus_tag="ACHK07_004015"
     CDS             complement(6742..7713)
                     /gene="rpnE"
                     /locus_tag="ACHK07_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO8104287.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSLLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7906..9096)
                     /gene="glpC"
                     /locus_tag="ACHK07_004016"
     CDS             complement(7906..9096)
                     /gene="glpC"
                     /locus_tag="ACHK07_004016"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO8104288.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9093..10352)
                     /gene="glpB"
                     /locus_tag="ACHK07_004017"
     CDS             complement(9093..10352)
                     /gene="glpB"
                     /locus_tag="ACHK07_004017"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO8104289.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10342..11970)
                     /gene="glpA"
                     /locus_tag="ACHK07_004018"
     CDS             complement(10342..11970)
                     /gene="glpA"
                     /locus_tag="ACHK07_004018"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO8104290.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQSLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12243..13601
                     /gene="glpT"
                     /locus_tag="ACHK07_004019"
     CDS             12243..13601
                     /gene="glpT"
                     /locus_tag="ACHK07_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO8104291.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13606..14682
                     /gene="glpQ"
                     /locus_tag="ACHK07_004020"
     CDS             13606..14682
                     /gene="glpQ"
                     /locus_tag="ACHK07_004020"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8104292.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3418 a         3680 c         4076 g         3763 t
ORIGIN      
        1 ctcagaaata tgtgaaagtg acagtaatct cttttgcgat gataactgca cttattatta
       61 tttttgtgtg tatcggtaaa gtgtgtttat tttttcggcg tttaattatt taagaagtat
      121 ttagttacaa taagtgacta gatccctttt ttcgctcatt gaatcctctg cgccaggcag
      181 tacaatcccg cagctttggg aattttgatt aattgcttgt gggaaagggg ataatgaaaa
      241 aaattgcgct agcaggtctg gccggaatgc tgttggtttc tgcatcggtc aatgcaatga
      301 gcatcagcgg ccaggtgggt aaagaataca ccaatattgg tgtcggtttt gggactgaat
      361 cgacgggcct ggctttaagc ggtaactgga cacataacga cgacgacggt gacgtcgcgg
      421 gcgtggggct ggggttgaat ctgcctctcg ggccgttaat ggcgaccgtt ggcggaaaag
      481 gcgtttacac caacccgaat tacggcgatg aaggttatgc cgcagcggta ggaggtggtt
      541 tgcagtggaa aattggcaac agcttccgtt tgtttggcga gtattactac tctccggatt
      601 cactctccag cggtattaaa agttatgagg aagcgaatgc tggcgcgcgt tacaccatta
      661 tgcgtccagt cagtattgag gcgggttatc gctacctgaa tctgtcgggt aaagacggta
      721 accgcgacaa cgccgtggct gacggcccgt atgttggggt taacgccagt ttctgatccc
      781 ttccggcgcg gcattctgtc gcgccattca gtttcttact acgccttatc tccctttgcg
      841 ttatagtgat tcgataacaa aagcaggaga taaccatgtt aaaagtggaa atgctatcca
      901 ccggggatga agtgttacac gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt
      961 ttttctttca tcaggggttg ccattatctc gccgcaatac ggtgggggat aaccttgatg
     1021 acttagtcac cattcttcgc gaacgtagtc agcacgccga tgtgctgatc gttaacggcg
     1081 ggctggggcc gaccagtgat gatttaagcg cactcgccgc tgcgacagca aaaggtgaag
     1141 gcctggtgct gcatgaagcc tggctcaaag agatggaacg ctatttccac gaacgtggac
     1201 gagtaatggc accgagcaac cgtaaacaag cggagctgcc tgccagtgct gaatttatca
     1261 ataacccggt aggcaccgcc tgtggttttg ccgtgcagct taatcgttgc ctgatgttct
     1321 ttactcccgg cgtaccgtca gaatttaagg tgatggtcga gcacgaaatc ctgccgcgtc
     1381 tgcgcgagcg tttttcttta ccacagccgc cggtttgcct gcgtttgact acctttggtc
     1441 gttcggaaag cgatctggca caaagcctgg acactctaca actgccgccg ggcgtaacaa
     1501 tgggctatcg ctcctcaatg ccgatcatcg aactgaaact caccggaccg gcaagcgagc
     1561 aacaggcgat ggaaaaactg tggctggacg ttaaacgtgt tgccggacag agcgtgattt
     1621 tcgaaggcac cgaaggactg cccgcgcaga tcagtcgcga attgcaaaac cgccagttca
     1681 gcctgacgtt gagcgagcaa ttcaccggtg gtttattggc tttgcaactt tctcgcgcag
     1741 gtgctccatt gctggcgtgt gaagtggttc cttcacagga ggaaaccctg gcgcaaactg
     1801 cgcactggat tacagaacgg cgggccaacc attttgccgg gctggcactg gctgtttcgg
     1861 gtttcgagaa cgagcatctc aactttgcgc tagccacgcc agacggcact ttcgctctgc
     1921 gtgtgcgttt cagcactaca cgctacagcc tggctatccg tcaggaagtg tgcgcaatga
     1981 tggcactgaa tatgctgcgc cgttggttaa acggccagga catcgccagt gagcatggct
     2041 ggattgaggt tgttgagtcc atgaccttat ccgtctgatt ccgccactct gtgccggttt
     2101 actgcaccgg cacattcccc ctccggcaaa atttgtccaa cctgttgtac tcaataagaa
     2161 aaaatccgtg cgctgattca caaatttgtg cgtaatttct ccagtccgtt ggacgaggag
     2221 cgggatttga tgcgcttcac aattttactc cccctgagca acgagactga ttccagtatc
     2281 taagactgga gtccagtatg ttggaatcaa gcaaagttcc cgccctgaca cgggctattg
     2341 atattctcaa tttgattgcc cgcattggcc cctgtaatgc tgcgacgatc attgacacat
     2401 tggggatccc taaaagcacg gcctatttgc tgcttaatga actcaggcgt cagcgttttc
     2461 ttagccttga tcaccaggaa aatttttgtt tgtggaccag gctggtggag ctttccggtc
     2521 atgcgttaag caagatggat ctccgggaac tggctcgacc gcgtttgacg caactgatgg
     2581 ataccaccgg gctgttatgt cacctgggga ttattgataa cggcagcgct tattacattt
     2641 tgaaagtgga gtcatcagcc actatcagcg tgcgttcaca tgaaggaaaa agcctttcgc
     2701 tttatcgctc cggaataggt aaatgcctgc ttgcctggca acctgcaact gtacagcaaa
     2761 gtattatcga agggctggtc tgggagcagg cgacacccac aacgattact catccacagc
     2821 aacttcacga agaactggcg cgtattcgcc gtcagggctg gagctatgac aacggcgaag
     2881 attacgccga tgtccgttgt gtcgctgcgc cggtgtttaa cgccaataac gagcttaccg
     2941 ctgcgatctc ggtggtcggt acccgcttac aaatcaatga agaataccgt gattatctgg
     3001 cgggcaaggc cattgcctgc gccagggata tttcacgtct gttgggatgg aaaagtccct
     3061 ttgacttaca agcctcataa caatggagaa catcatgacc ctaccaaaaa ttaaacaggt
     3121 tcgcgcctgg tttactggcg gtgcgacagc agaaaaaggc gctggcggcg gtgattatca
     3181 cgaccagggg gcgaatcact ggatagacga tcatattgcc accccgatga gtaaataccg
     3241 cgattacgag cagtcacgcc agtcatttgg cattaacgtt cttggcacgt tggttgttga
     3301 agtcgaagca gaaaacggcc agaccggatt cgccgtttcg acagccggtg aaatgggctg
     3361 ttttatcgtc gaaaaacacc ttaaccgttt cattgagggt aaatgtgtca gtgatatcaa
     3421 actgatccat gatcaaatgc tcaatgccac cctgtattac tccggctctg gtggcctggt
     3481 gatgaacacg atttcctgtg tcgatctggc tctgtgggat ctgttcggca aagtggtcgg
     3541 gcttccggtt tataaacttt taggcggcgc tgttcgtgat gagattcagt tctacgccac
     3601 aggtgcgcgt ccggatctgg caaaagagat gggctttatc ggtggcaaaa tgccgacgca
     3661 ctgggggcca catgatggcg atgcggggat ccgcaaagat gccgctatgg tcgcggatat
     3721 gcgtgaaaaa tgcggtgagg atttctggtt aatgctcgac tgctggatga gtcaggacgt
     3781 gaactatgcg accaaactgg cccacgcttg cgcgccctat aacctgaaat ggatcgaaga
     3841 gtgcctgccg ccacagcagt atgaaggtta tcgcgaactg aaacgcaacg cgccagtcgg
     3901 aatgatggtc accagcggtg agcaccacgg cacactgcaa tcttttcgta cgctttcaga
     3961 aaccggtatc gacattatgc agccggatgt tggctggtgc ggtggcttaa ccacgctggt
     4021 ggaaattgcc gcaatcgcca aatccagggg gcaactggtg gtgccgcacg gttcgtctgt
     4081 ttactcccac catgcggtga tcaccttcac taatacgcca ttcagcgaat tcctgatgac
     4141 cagtccggat tgttcaacga tgcgtccgca gtttgacccg attctgctca atgagccggt
     4201 tccggtgaat ggtcgtattc ataaatcagt gcttgataaa cccggttttg gcgtcgaact
     4261 caatcgtgac tgcaatttga aacgccccta cagccactaa tcactccggg cgttgctgtg
     4321 acaacggtaa cgctcacata ttctaaggag ctttttatga gcaccgcttt gcttgacgcc
     4381 gtggtgaaga aaaaccgtgt gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg
     4441 tttctcgacc gttcgaatat cggttttgcc aaacagacct accagattga taccgggttg
     4501 agtaatgaag cttatgcgct gggagcaggc attttctttg tggtatatgc gtttctgggt
     4561 gttccggcga atcttttgat gcgcaaactg ggggccagaa cctggattgg tacgacaaca
     4621 ctgctgtggg gatttctttc ggcagccatg gcatgggccg atactgaagc gaaatttctg
     4681 atagttcgca ctctgcttgg tgctgcggag gccggatttt tccctggtat gatttatctc
     4741 acctcgcaat ggtttccgca gcgtaatcgc gccagcatta tggggctgtt ctatatgggc
     4801 gcaccgctgg cgttaacact gggatcaccg ctttccggcg cgctgctgga gatgcatgga
     4861 tttatggggc atcccggctg gttctggatg tttgtgattg aaggattgtt ggcagtcggc
     4921 gctggggtat tcacattctt ttggcttgat gacacaccgg agcaggcacg ttttctgagt
     4981 aaacaagaaa aaacgttgct tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact
     5041 tctcggctga gcgatgcgct gcgtaatggg cgagtctggc aactggcgat tatctacctg
     5101 accattcagg tggcggttta cggattaatt ttcttcctgc cgacccaggt tgcggcattg
     5161 ctgggaacaa aagtgggctt tacagcgtcg gtggtcaccg ccattccgtg ggttgcggcc
     5221 ttgtttggga cctggcttat tccgcgctat tccgataaaa ccggcgaacg gcgtaatgtc
     5281 gcagcgctga cattactggc ggcgggcatt ggtattggtc tgtccgggct gctttctcca
     5341 gtaatggcga tcgtagcgct gtgtgttgca gccatcgggt ttattgccgt gcagccggtg
     5401 ttctggacga tgccgacaca actgctttcc ggtacggcgc tggctgcggg gattggtttt
     5461 gtaaacctgt ttggtgcagt gggcgggttt attgccccga tcctgcgcgt gaaagcagaa
     5521 acgttatttt ccagcgatgc ggcgggatta ctgacgctgg cagcggtggc ggtcatcggt
     5581 tcgctgatta ttttcactct gcgtgtaaat cgtactgttg cgcagaccga cgtggcacat
     5641 cattaaatag gttaaggaac acgatgaacg cattattaac caatcccttt aaagaacgtt
     5701 tacgcaaggg cgaagtgcaa attggtctgt ggttaagctc aacaactgcc tatatggcag
     5761 aaattgccgc cacttccggt tatgactggt tgctgattga tggggagcac gcgccaaaca
     5821 ccattcagga tctttatcac cagctacagg cggtagcgcc ctatgccagc caacccgtga
     5881 tccgtccggt ggatggcagt aagtcgctga ttaaacaagt cctggatatt ggcgcgcaaa
     5941 ctctactgat cccgatggtc gatactgccg atcaggcacg tcaggtggtg tctgccacgc
     6001 gctatcctcc ctacggtgaa cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg
     6061 gacgcattga gaattacatg gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg
     6121 aaagtaaaac ggcactggat aacctggacg agatcctcga cgtcgaaggg attgatggcg
     6181 tgtttattgg acctgcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc
     6241 cggaagtgca gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg
     6301 ctggttttct ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact
     6361 ttgtcgctgt tggcgttgac acgatgctct acagcgatgc cctggatcaa cgactggcga
     6421 tgtttaaatc aggcaaaaat gggccacgca taaaaggtag ttattgatat caaaggccca
     6481 tggggatcgg ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct
     6541 tagctgggtg gccgcgagca cctggtcacg atcaatgccc tgttccagca tgcgcaaggc
     6601 aattttaata gcttgttcat gcatgccttc ctgcaaacct tctaatttac cttgctgcaa
     6661 acctactaat ttaccttctt gccagccaat ttgatgtcct tcctgccgta gccgttcagc
     6721 aatagtcatc aatctctcct tttaatgaca gttcggaata tcagcatcag tgagttgggt
     6781 tgccgccagc acaatctcac gttcaaagcc ctgctccagc atgcgcaagg caattttaat
     6841 ggcttgctca tgcatgcctt cctgcaaacc ttctaattta cctttctgca gaccctccaa
     6901 tttaccttgc tgcaaacctt ctaatttacc ttcctgccaa ccaatttgat gtccttcctg
     6961 ccgtagccgt tcagcaatag tcattaatct ctccttttgt agtagtgaac gttcggcaat
     7021 ctcctgaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag
     7081 tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac
     7141 cataccgatt aaatcgcggt cgcgaatatg cttttgaatc agttcaagca gagctatacg
     7201 ccgatgttgc atgatctcat cgtcaggtac gatggtaata tccaccaacg gaaaagcttc
     7261 ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt tgagtgagta
     7321 cgggtagggc gaggtttcgc catgataaaa cagcaacggc accaccagcg ggactctgtc
     7381 atagcctttg tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc
     7441 catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc
     7501 gctggtttcc acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa
     7561 actggtgggt tccaggcgta aggtttgcag gttgcaaagc ttgcgcagcg gctctggtag
     7621 atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaatactgc
     7681 atcatgcggg gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat
     7741 gacgcgacat tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac
     7801 gttttcagag gaattttcac tgcgttgcaa cgcatacatt gggcacggaa tcgtgtacgc
     7861 aaaaatgccc ggtaaccaca tgcttaccgg gcatcagaaa ggagtttaag ccagcgcctg
     7921 ggccagtagc gtaatcggat gttcgcagcg aagacttgtg gacatctcaa tctgccattt
     7981 acaggtttcg cagtcggtga ccaccagatc tgcgccgctt tcttctatct ggcggaacag
     8041 tggtgcgccg atggcttgtg aggtggggta gttctctttt ttgaaaccgt aagtacccgc
     8101 aataccgcag cactgggaat ccagcaccgt taactcaagc cccgggatgt tacgcaacag
     8161 ctccagggtg tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac
     8221 tttcagcggc agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca
     8281 gcgggtcgcc agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg
     8341 gtactcgtcg cgcagggcga aagtacaggt ggaggaggtg gcaatcaccg gaatgccttt
     8401 tactcccacg gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc
     8461 ggtaaagccg ttggcgatta gcggcacacc gcagcatttt tctttgctga gcagttgcac
     8521 gccagtaccc atcgcgttga gcactttaat taaatcttta cctaactgcg gatggttgta
     8581 gttaacgaag cagccgtgga aaaaggcgac ctggtcttta tactgcgcct gctgggccgc
     8641 cacgctgcga taccagcgac gaaacgtgcc gaaggagtat ttcggtagcg tgcggcgatg
     8701 atcgattttt aacgccgcat caagcaactg ccgcaccggt ttcagcgagg tagcggtgtt
     8761 gacgattggt gcgaacggcg tggaaacgct acccatcagg tcggtatggc ttaaaacaaa
     8821 attgcgcagc gacgggcgcg tggtgtcata tttcgcccgt gcgcgctgga tgatatcgcc
     8881 aatcttcaca tcggacgggc aggcgacttc gcaacgtttg cagttgatgc aatatttcag
     8941 tgcctcgtca tacagtgcgc catcttttaa acgcagacgc tcgccatccg gcccggcttg
     9001 ttttggccct ggataaccgg gattcacccg gctcactggg caggcggtgg tgcagacggt
     9061 gcacttaatg cagttttcga agctggtgtc attcattgtt ggcctcctgc gcgttgggca
     9121 atctgttgag cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg
     9181 atgggatcaa atccgcccag caccgaaccg atggcaaaca ggttttcgat ggtttgccct
     9241 gcctgcgacg ggcgtagcgt ctgatcagtg gttacaccga actgctgcca cggttgcggc
     9301 gcaaaaaaat ctcccttata ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg
     9361 agaatcggct ctcgaatgcc gttacgttcc gctaccagtc cgccactaaa gaaactgccg
     9421 ctggcgagaa ccgcgaaacg tggacgcagc gggatatcgg cgtgattgcg ggtccagatt
     9481 tcattcacta cgccattttt acaggtcact tttttcactt catcgcccgg catccacacg
     9541 ccaccctgac gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa
     9601 ggcggcagcg ttggcaaaag catcagtgaa caaggtagtt tttcattcaa ccaacgccac
     9661 agtttgtcat cagccaggcc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg
     9721 acaggaataa gcgcatcaag taacagcggc cagttttctt cattatcaag gaaacgggcg
     9781 atattcaccg cgcggaactc ggtggcgtta ttgcgcagga catccagttc cggcagctct
     9841 atttctgcgg tttcaacggc aaggccgagt tcacgcaacg aagctgccgc aaggtgcgcc
     9901 tgaaaatcca tcagtccact aattcccact acacatattt tcttcgcggg cagcggccag
     9961 acggggactt ctggcgaact tagccaggta gagcgcagag tgccgagcgg cgtaatccgc
    10021 tggtgagcca gttctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc
    10081 gcctggcaag cgagatcgag cacgcgttgt ggcccgagaa gagagtaagg atgtgcaggt
    10141 gcctgctgac gcaaagattc cagtccactg tgaatgtctg tcaccggttg accatctggc
    10201 agatggctca gcaaatccag cgaaccggat gagaaatgca gcgcgctttg accacgcgtg
    10261 acaatggcac agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa tccggcgagg
    10321 ccgccgccca taatgacagt atcaaagcgc atctttctgc tccttctcca gaccacacaa
    10381 tccctgataa acccagcggg taaattcgct ttcgcgcagt gcatctcccc aggcgatggg
    10441 ttgcacgcct ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga
    10501 cgtcgtgacg ttaaaacgtt gcagcagtcc ggcagcgcgg caggcgcaga gttcgccctg
    10561 gcaggtaccc atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa
    10621 attttctacc gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct
    10681 acggtgcaga cggccttcgc tcagccaggc aggcgtacga tcgccatgac gataaaccgc
    10741 agaaccgcgc agcggggcag gcagggagat gactttacgc aaggtaactt cagcgggttc
    10801 ctgtgaaccc ggaagtgcca gatcggcggt cgtacagggg cgcgtgttgc ccagtttgcg
    10861 gcatacggcg tcggtagccc attccgccat cagccgatag gtcatcagtt tgccaccggt
    10921 aatggtgata aatccgtcca gaccatcgcg ttcagcatga tcgagcagca cgatgccgcg
    10981 gctgacgtta cgcccgctcg gatcatcatc gctggcgacc agtggacgca cgccagagta
    11041 ggcccgcaaa atgcgcgttt gcgccatcac gggggccagt ttttcccctt cacgcagcag
    11101 gatatccact tcttctgccg tcacccgatt atcgtcaatc tcgttgtaat caatacgtaa
    11161 agaggtggtg ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg
    11221 tttacggcag cggttgatca catgctggtt aatgcggtga tccatgatca gcagcgatcc
    11281 tttcgccggg aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat
    11341 cccggcagcg ttaaccacga caggcgcatg tagcgactgg gtttcgccgg tgaggtggtt
    11401 gcgtacatga acgccgcaca ccgttgcgcc ttcacgaatc agtcccgtga cttcatgagc
    11461 agtaaggata acggcaccgt gttctttggc atccagcatg tttgctgcgg tcagacgaaa
    11521 tggatcaacg gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg
    11581 ttcgataatg cgcgcttgct gcgggtctat agcttctgcg ctgatccctg cttcttcgca
    11641 ggcgcgaata aaagtggcct ggaaggagag gtcatcttcc ggcagggtga taaacaggcc
    11701 gttggttggt tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt cactaatgca
    11761 ttcgcgggcc gattccgcat cggttaccgc gtagcgcgca ccgctgtgca gcaggccgtg
    11821 gttacgcccg gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc
    11881 gcgcagggca cagtcgcggg caatcccggc tcccgttgcg ccgccgccaa tgataatcac
    11941 gtcacttgat tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca
    12001 tttagccata gtaaaaacat gaattgtttg atttcgcgca tattcgctca taattcgaaa
    12061 gtgaaacgtg atttcatacg tcattttgaa cattctgtaa atcttattta ataatgtgtg
    12121 cggcaattca catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc
    12181 aggttctctc actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggctat
    12241 caatgttgag tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg
    12301 atccgactta tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgctg
    12361 cttactattt ggttcgtaag aactttgcgc ttgctatgcc ttatctggtt gagcagggat
    12421 tctcacgcgg tgatttaggt tttgcccttt cggggatctc gattgcttat ggattttcga
    12481 aattcatcat gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt
    12541 tgattctggc ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca
    12601 ttgcggtgat gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc
    12661 cgtgtggtcg tactatggtg cactggtggt cgcagaaaga acgtggcggc attgtgtcag
    12721 tgtggaactg tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga
    12781 tggcctggtt caatgactgg cacgcggcgc tctatatgcc cgctttttgc gccattctgg
    12841 tggcattatt cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ttgccgccga
    12901 tcgaagagta caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga
    12961 ccgcgaagca aatcttcatg cagtacgtgc tgccgaacaa actgctgtgg tacatcgcca
    13021 tcgccaacgt gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc
    13081 tgaaagaggt taagcatttc gcgctggata aatcctcctg ggcctacttc ttctatgagt
    13141 atgcgggtat tccgggcacc ctgctgtgcg gctggatgtc ggataaagtc ttccgtggca
    13201 accgtggggc aaccggcgtt ttctttatga cactggtgac catcgcgact atcgtttact
    13261 ggatgaaccc ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt atcggcttcc
    13321 tgatctacgg ccctgtgatg ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag
    13381 cggcaggaac ggcagcgggc tttaccggtc tgtttggtta cctgggcggt tcggtggcgg
    13441 cgagcgcgat tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa
    13501 tgattggcgg cagcattctg gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac
    13561 gtcgccatca ggaattactg caaaaacgca acggaggcta atggcatgaa attgacgctg
    13621 aaaaacctta gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg
    13681 gcggacagca acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag
    13741 catacgctgc cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat
    13801 ttggtgatga ccaaagacga ccatctggtt gttctgcatg accattacct cgatcgtgtt
    13861 actgatgttg ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata
    13921 gatttcacgc tggatgaaat taagtcgttg aaatttaccg aaggtttcga tattgaaaac
    13981 ggtaaaaaag tgcagactta tccagggcgt ttcccaatgg gtaagtccga cttccgggta
    14041 cacacctttg aagaagagat tgaatttgtt caggggttaa atcactctac cgggaaaaat
    14101 atcggtatct atccagaaat caaagcgccg tggttccatc atcaggaagg gaaggatatt
    14161 gcggcaaaaa cgctggaagt gctgaagaaa tatggttaca ccggtaaaga cgataaagtt
    14221 tatttgcaat gttttgatgc tgatgagctg aagcgtatta agaatgagct ggaacccaaa
    14281 atgggcatgg atctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag
    14341 cagaaacagc cggacggaag ctgggttaat tacaactacg actggatgtt taagccgggt
    14401 gccatgaaac aggtggcgga atatgcagat ggtattggtc cggattacca tatgttgatt
    14461 gaggagacat cgcagccggg taatatcaaa ctcactggca tggtgcaaga tgctcagcag
    14521 aacaagctgg tagtgcatcc ttataccgtg cggtcagata aactgcctga atacacaact
    14581 gatgtgaatc agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gttgtttact
    14641 gatttccctg ataaagcggt taaattcctt aataaagagt aatgctgcta ctgcaggatg
    14701 gcgttcatgc gccatcctct gctaaagcga aataatgcta ctaccggtta ttcttttcat
    14761 taaccggtgc atcctgtaaa aaagatgcca acataatttt cgtaaaactc attcgttaaa
    14821 aaagctaaca ctgggcattg ttattcttta aacagtatga ataggtctca ataataaatt
    14881 gtaatgcagt ttatactgta aagtattatt ctaatttcgg aaaggtaaag aaaatat
//
LOCUS       DAZGCQ010000112        14674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000112 DAZGCQ010000000
VERSION     DAZGCQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.112"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            495..944
                     /gene="alaE"
                     /locus_tag="ACHK07_004021"
     CDS             495..944
                     /gene="alaE"
                     /locus_tag="ACHK07_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO8104293.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKISPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(981..1325)
                     /locus_tag="ACHK07_004022"
     CDS             complement(981..1325)
                     /locus_tag="ACHK07_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO8104294.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1477..1806
                     /locus_tag="ACHK07_004023"
     CDS             1477..1806
                     /locus_tag="ACHK07_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO8104295.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1956..3290)
                     /locus_tag="ACHK07_004024"
     CDS             complement(1956..3290)
                     /locus_tag="ACHK07_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8104296.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGENQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3378..3809
                     /locus_tag="ACHK07_004025"
     CDS             3378..3809
                     /locus_tag="ACHK07_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO8104297.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIAKT
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            4018..4263
                     /gene="nrdH"
                     /locus_tag="ACHK07_004026"
     CDS             4018..4263
                     /gene="nrdH"
                     /locus_tag="ACHK07_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO8104298.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4260..4670
                     /gene="nrdI"
                     /locus_tag="ACHK07_004027"
     CDS             4260..4670
                     /gene="nrdI"
                     /locus_tag="ACHK07_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO8104299.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4643..6787
                     /gene="nrdE"
                     /locus_tag="ACHK07_004028"
     CDS             4643..6787
                     /gene="nrdE"
                     /locus_tag="ACHK07_004028"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8104300.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VVDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6797..7756
                     /gene="nrdF"
                     /locus_tag="ACHK07_004029"
     CDS             6797..7756
                     /gene="nrdF"
                     /locus_tag="ACHK07_004029"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8104301.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKRLEKISEAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            8112..9314
                     /gene="proV"
                     /locus_tag="ACHK07_004030"
     CDS             8112..9314
                     /gene="proV"
                     /locus_tag="ACHK07_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO8104302.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            9307..10371
                     /gene="proW"
                     /locus_tag="ACHK07_004031"
     CDS             9307..10371
                     /gene="proW"
                     /locus_tag="ACHK07_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO8104303.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10429..11421
                     /gene="proX"
                     /locus_tag="ACHK07_004032"
     CDS             10429..11421
                     /gene="proX"
                     /locus_tag="ACHK07_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO8104304.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11613..12797
                     /locus_tag="ACHK07_004033"
     CDS             11613..12797
                     /locus_tag="ACHK07_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104305.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYNDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12921..13658
                     /gene="ygaZ"
                     /locus_tag="ACHK07_004034"
     CDS             12921..13658
                     /gene="ygaZ"
                     /locus_tag="ACHK07_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO8104306.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13648..13983
                     /gene="ygaH"
                     /locus_tag="ACHK07_004035"
     CDS             13648..13983
                     /gene="ygaH"
                     /locus_tag="ACHK07_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO8104307.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            14074..14604
                     /gene="emrR"
                     /locus_tag="ACHK07_004036"
     CDS             14074..14604
                     /gene="emrR"
                     /locus_tag="ACHK07_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO8104308.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
BASE COUNT         3547 a         3758 c         3687 g         3682 t
ORIGIN      
        1 gatgagtaat ttaggttatt ggtgaacaga gtggcgctct ctgctcgcta attccgcaag
       61 cctgacaaaa ccgaagtgaa taaaaatgca atataacaga ataaaagaaa acaccactca
      121 ctggtgcttt ctgctgttaa tgtacatttt ctggcataaa aaatgctgta aatgacccgg
      181 tcacgacata caccgaattc tctacgctta tagcgttacc tcacccccaa actttcatta
      241 gtgaaattaa aggaaaggtc atatctaatt gatttaattg aaaataaatc atatcggcat
      301 gtaaaaatcc acaatgtaca aaaaacgagc cgttacggaa tattttatct acaaaaactg
      361 actaaataaa aatttttcac taattgatta gtcatagtca gcgatatacg ctatgcgaaa
      421 atgcagatgg caatgagatc cactgctttc atctccatta acatcccatt acgcttttat
      481 taaggagcat tagcatgttc tcaccgcagt cacgcttgcg tcatgcagtt gcagatacgt
      541 tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat tgaagttttc ctctccggaa
      601 tgagcttcga acagtctttt tattccagat tggtagcgat tccggtgaac atcttaattg
      661 catggccata cggtatgtac cgcgatctgt ttatgcgcgc ggcacgcaaa attagcccgt
      721 cgggctggat aaaaaatctg gcggatatcc tggcttatgt gacgttccag tcaccggtgt
      781 atgtggcgat cttgttagtg gtgggcgcag actggcatca gattatggcg gcggtcagtt
      841 caaacatcgt tgtttcgatg ttgatggggg cggtttatgg ctacttcctc gattattgcc
      901 gccgactgtt taaagtcagc cattaccagc aggtaaaagc ctgactcttc ttttcgcgac
      961 tggcatcgcc agtcgcgtct ttaactggct tcgccaaata aaccattcaa ataacgttca
     1021 agcgcaacac gcgaactaaa gccatgcggg ataccgtaat gctcgtgtcg ctcgcctgct
     1081 aaatagagcg gaaattgttg ataatgatcg caagttttaa tatcggaagc gggttcagcc
     1141 agtgtcgaac tacgttcttt taatgttgga tgaattacca gtgcggtacg tcccattcgc
     1201 gcttcgcgat taacatagac ataattttcc ccacggcggt aaccatacgc tttctgggtt
     1261 accacatcga cagtaaaacc gactttttca agtacgcgcg ccacctcgtc tggtcgtaaa
     1321 tacatatttg atcctcgtta ttatcaaccg cgggcctacc ttacctgatt gcgcattcac
     1381 aacgctttca gaaaagtcca taaagccgcg atgaacctca gttaagaaaa ttatggtcta
     1441 cactgaaaat tacatcgaat tctaatgggg gatcatatgt ttaaccgacc gaaccgcaac
     1501 gacgttgatg atggcgtgca ggatatccag aatgatgtca atcaattagc tgacagcctc
     1561 gaatctgtat tgaagtcctg gggcagcgac gccaaagggg aagctgaagc cgcacgcagt
     1621 aaagctcagg cattgctgaa agaaaccagg gcgcgaatgc atggtcgtac tcgcgtccag
     1681 caagccgcac gcgatgccgt tggctgcgcg gattcttttg ttcgtgaaag accctggtgt
     1741 agtgtgggaa ctgctgctgc ggtaggtatt tttatcggtg cgttgttgag catgcgtaaa
     1801 tcgtagtgag acgatgacaa gcagtagtct gacttctgaa aagtactcag tgcgtcaacg
     1861 ccttgtccgc ctacaggcct gaaaaacata gcgcatcagg ggattttgcc ctggtcacca
     1921 gtcttatagg accccgaagc ctggcttcgg ggttattaat ttaaagcact gcgcaattgc
     1981 tgtgctacct gtcgcgccat actttcgcta acgatattcg taaacgagag caataatcct
     2041 ccgtcctcgc ttgagcggat acgccagtcg ctaagtgcct gtacggcaag accagcagca
     2101 ttggctttat gcacgaatgt cacgtcatta cctgctagtt tgatcaccat ctggataccg
     2161 cctttttgcg gcacaacctg aaatccctgc tgcgtaagtg cctgctcaat ccactgccgt
     2221 cgctgggcat aatgctggcg cattttcttc agatgccgcc agaaatgccc ctcacggatg
     2281 aaatctgcca gtgtgttctg ccacagtacg ggtacagcac atggttccag tgacgcctgc
     2341 tgccggaatt gcgaaatctg ctccaccggc accaccagcc acgcacagcg caatgcagga
     2401 aatagcgctt tgctgaatgt tccggcataa atcacccgct gcggcgcgtc gagacttttt
     2461 agtgccggta acggcttacc gtgatagcga aactcactgt cgtaatcatc ttcaataatc
     2521 catgcctgac tacgctctgc ccattccagt atctgatacc tacgcgctaa agagagtgcc
     2581 acacccagcg ggctttggtg tgccggagta atcagggcaa aacgcgcatc aggataattt
     2641 tgtattccgc ttgtgacatc cagtccgttg tcatcaaccg gcacaggtaa aatttccaca
     2701 ccgtggcgag tgacaatcgg gcgaatcagc ggaaagccgg gatcttccat ccacatcccg
     2761 tctcccggtt gcgccagagc gtgcagaatt aaagccattg aggccgcata accgtgagtg
     2821 ataaagacct gctctggctg acaatcaatc ccacgtgaaa cgcgtaaata atcaacaata
     2881 gcctcgcgca aggccgcctc gccgcagaca tcgcctaatg ccaaatcaaa acgcgtctgg
     2941 gtacgcagac gacggcccat cacccgcgcc cacaattcac gcggaaaaag atcaagtgca
     3001 ggtagcccca tctgaaaagg cagcggctgc tgattttcac cagcaaaagc cacgacatcc
     3061 acagcagact gttgcggatg tatctgctca ctgacaaagg tacccgcctg tccccgcctg
     3121 accagccatc cctgcgccac cagctcagca taagcatttt cgacagtgga tcgagagaca
     3181 cccagctcct gcgaccaggt acggctcgag ggtagccttg cgccaggttt cagttctcct
     3241 tgctcgatag ccgtttttaa ctgacgggcg atatcctgat aacgtggcat atggtctgct
     3301 tttttaggta atttatggct ctctttagca gtccattata gtgctacatt gcgggcgtta
     3361 ataagaggag aaactgtatg actacattac gtcagcctta ctacgaactt agcccggcag
     3421 tgtataacgc actggtgcag gccaaaacgg cactggaaaa tagcacgctg gataccacgt
     3481 tgatggagtt aatttatttg cgcgtctcgc aaatcaacgg ctgcgcattt tgtctggaga
     3541 tgcacagcaa agcattgcgc aaatccggtg tgccacaaca caaactggac gccctggcag
     3601 gctggcgcgt aagccatcat tttgatgaac gcgagcgagc ggcgctggca tgggcggaat
     3661 cagtgaccga tattgccaaa acccatgcgg aagacgaggt ttatcagcct ttgcttgagc
     3721 atttcagcgc agcagaaatc agcgacttaa cgtttgccat cgggctgatg aattgtttta
     3781 accgtctggc cgtttccatg cggatgtaat ttgctgttgc cgggtatgcg cccggcaaaa
     3841 aaacacaaaa tcccctaccc cgttgagcca ttcaccggtg gcgtaatcaa tctaaaattc
     3901 ccatatcttg tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag
     3961 agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg
     4021 cgcattacta tttacactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg
     4081 gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcggcagaa
     4141 gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga tcttagctgg
     4201 tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc ggccagtgca
     4261 tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt atcgaacgtt
     4321 taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc
     4381 cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac
     4441 aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc gttattgctt
     4501 cgggtaatcg caactttggt gaggcgtatg gccgcgctgg agatgtgatt gcccggaaat
     4561 gcggcgtgcc gtggctgtac cgctttgaac tcatgggtac gcaaagcgat atcgaaaacg
     4621 ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga cgcaggaaac
     4681 gatggattac cacgcgctga atgcgatgct taacctctac gatagcgcag gtcgcattca
     4741 gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc gtccgaacag
     4801 tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag gttactatga
     4861 tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg accacgcaca
     4921 caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca ccagctatac
     4981 gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag tcacgatggt
     5041 ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgacag atgaaatgct
     5101 gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg
     5161 cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga ttggtcgggc
     5221 ggtaaattcc gcactgcaac tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa
     5281 tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtaattcc
     5341 ggtgatgaaa atgctggaag acgcattttc ctatgccaac caactcggcg cgcgtcaggg
     5401 ggctggggca gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgacacgaa
     5461 acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgcttggcg tggtgatccc
     5521 ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt cgccttatga
     5581 cgtagagcga gtttatggca agccgtttgc cgatgtggcc atcagtcaac actatgacga
     5641 actggttgtc gatgaacgca ttcgaaaaaa atacctcaac gcccgtgatt tcttccagcg
     5701 actggcagaa atccagtttg agtccggcta tccgtacatc atgtatgaag acacggtaaa
     5761 ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag agattttgca
     5821 ggttaacagc gcctcagagt ataacgagaa tctcgactat gcccgcacag gccatgatat
     5881 ctcctgcaat ttaggttcgt tgaatattgc ccacaccatg gattcccccg actttgcccg
     5941 cacggtagag actgccgtgc gcggtttaac ggcagtatca gatatgagtc atatccgcag
     6001 cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg ggcagatgaa
     6061 tttacacggc tatctggcgc gagaaggcat cgcttatggt tcgccggaag cactggattt
     6121 caccaatctc tatttctatg ccatcacctg gcacgcactg cgtacctcga tgttgctggc
     6181 acgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gcggtgaata
     6241 ttttagccaa tatctgcaag gtaactggca gccgaaaacc gcgaaagttg gcgaactgtt
     6301 tgcccgcagc ggtattacgt tacctacccg tgagatgtgg gcgcagctgc gcgatgacgt
     6361 gatgcgctac ggcatttaca accagaatct ccaggcggtg ccgccaaccg gttctatctc
     6421 ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa
     6481 agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg agaatctggc
     6541 gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct acgcggaagc
     6601 gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac
     6661 tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa cgctctatta
     6721 catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc
     6781 actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc
     6841 tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa
     6901 ggtgccactg tcgaacgata tacctgcatg gcagacatta agtgtcgtag aacaacaact
     6961 gacaatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc
     7021 gccttcgctg atgcccgatg cgctcacgcc ccatgaagag gcggtattat cgaatatcag
     7081 ctttatggaa gcggttcatg cccgttctta cagttcgatt ttctcgacgt tgtgccagac
     7141 caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc agcgaaaagc
     7201 tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt
     7261 gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg
     7321 cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg aagcagtcca
     7381 cggttactac ataggctata aatatcagaa aagactggaa aagatctccg aggcacaacg
     7441 tgaagagttg aagagtttcg ccttcgattt gttgctagaa ctctatgaca acgagttgca
     7501 atataccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag cgtttctctg
     7561 ttacaacgcc aataaggcgt tgatgaatct gggctacgaa cctttatttc ccgcagaaat
     7621 ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga
     7681 tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa cagaagatga
     7741 agactggaat ttctgagggt gttattttca aaaatatcac tacccgcgac agggaaataa
     7801 ttcccgccaa atagcttttt atcacgcaaa tattttgtgg tgatctacac tgatactctg
     7861 ttgcattatt cgcctgaaac cacaatattc aggcgttttt tcgctatctt tgacaaaaaa
     7921 tatcaacttt tctcgatttg ctctcagccc ttatatcacg ggaaattccg gcgatttgct
     7981 cacatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc agtatgttag
     8041 ggtagaaaaa agtgactatt tccattgggt aatgtatcga cataaacaaa taacaggaat
     8101 ctttctattg catggcaatt aaattagaaa ttaaaaatct ttataaaata tttggtgagc
     8161 atccacagcg agctttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa
     8221 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
     8281 ttgtcatcat gggattatcc ggttcgggta aatccacaat ggtacgcctt ctcaatcgcc
     8341 tgattgaacc cacccgcggg caagtactga ttgatggtgt ggatattgcc aaaatatccg
     8401 acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
     8461 taatgccgca tatgaccgtg ctggacaata ctgcgtttgg tatggaattg gccggaatta
     8521 atgccgaaga acgccgggaa aaagccattg atgcactgcg tcaggtcggg ctggaaaatt
     8581 atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc
     8641 gcgcgttagc gattaatccg gatattttat taatggatga agccttctcg gcgctcgatc
     8701 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
     8761 ccattgtctt tatttcccac gatctcgatg aagccatgcg tattggcgac cgaattgcca
     8821 ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc aataatccgg
     8881 cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga
     8941 aagatattgc ccgccggaca ccgaatggct taattcgtaa aacccctggc ttcggcccac
     9001 gttcggcact gaaattattg caggatgaag atcgtgaata tggctacgtt atcgaacgcg
     9061 gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta acgcagcagc
     9121 aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta
     9181 gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc
     9241 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
     9301 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgcg
     9361 caatccgcag acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggtgctgac
     9421 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
     9481 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt cgttacccat
     9541 ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag
     9601 caattgctgc tgggtatgcc cgcgccggtg gcgattatcg ttttcgctct catcgcctgg
     9661 cagatttccg gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca
     9721 atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaaccgc cctgctgttc
     9781 tgtatcgtca tcggtttgcc gttggggatc tggctggcga gaagtccgcg agcggcgaaa
     9841 attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca
     9901 atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgct
     9961 ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt
    10021 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
    10081 ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
    10141 gtggtcatcg cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc
    10201 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
    10261 atcctcgatc gtctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc
    10321 tggtacacca ctggccctgt cggtctgctg acccgcccat tcattaagta actctgcact
    10381 tgcccggtga caccgggcat atcaccctgc caaaaaaaag gaataacaat gcgacatagc
    10441 gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc tgccgatctg
    10501 ccgggcaaag gcattactgt taatccagtt caaagcacca tcaccgaaga aaccttccag
    10561 acactgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaataa acccagcgaa
    10621 gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt caccgccgtg
    10681 aactggacac cactgcatga caacatgtac gaagctgccg gtggcgataa gaaattttat
    10741 cgtgaagggg tatttgttaa cggcgcggca cagggttacc tgatcgataa gaaaaccgcc
    10801 gaccagtaca aaatcaccaa catcgcacaa ctaaaagatc cgaagatcgc caaactgttc
    10861 gataccaacg gcgacggtaa agcggatttg actggctgta accctggctg gggctgcgaa
    10921 ggtgcgatca accaccagct tgccgcgtat gaactgacca ataccgtgac gcataatcag
    10981 gggaactacg cggcgatgat ggctgacacc atcagtcgtt acaaagaggg caaaccggtg
    11041 ttttactaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg gaaagatgtg
    11101 gtctggttgc aggtgccgtt ctctgcactg ccgggcgata aaaatgccga taccaaactg
    11161 ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc caataaagcc
    11221 tgggccgaga aaaacccggc agcagcgaaa ctgtttgcca ttatgcagtt gccagtggca
    11281 gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg cgatattcag
    11341 ggccacgttg atggctggat caaagcccac cagcaacagt tcgatggctg ggtgaatgag
    11401 gcgttggcag cgcagaagta atttttattc gggcggataa ggcgtttacg ccgcatccgc
    11461 cgttctgtac acaatgcctg atgcgacgct ggcgcgtctt atcatgccta caaaccacac
    11521 cgcacatttc aacaatctat ctttcatccc atattcatca acatccgcta ttattgattt
    11581 ccagcttaat catcacctga tgaacaaaaa taatgaccaa acctaatcat gagcttagcc
    11641 cggcgctaat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt aactattacg
    11701 cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt tcggcaggct
    11761 ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt gttcccctcg
    11821 gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggcg gcatgcggca
    11881 tgttgattac cgccagcagt cagtcgctgg cgatgatgat cctcggtacg gcattaaccg
    11941 gtttattctc agtcgtggca caaattctgg ttccactggc agcgacgctg gcttcaccgg
    12001 ataaacgcgg caaagtggtt ggcaccatta tgagcggcct gctgttgggg atattgctgg
    12061 cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc ttttgggttg
    12121 cctcggtgtt aatggctctg atggcgctag cattatggcg tggtctgcca caaatgaaat
    12181 cagaaaccca cctcaattac ccacagttgt tgggttccgt tttcagtatg tttatcagca
    12241 ataagatcct gcgcacccgc gcgttgctgg gctgcctgac ctttgccaac ttcagcattc
    12301 tctggacctc aatggccttt ttgcttgccg caccgccttt taattacaac gatggcgtca
    12361 ttggtctgtt tggacttgcg ggggctgccg gagcgttggg cgctcgtccg gcgggcggtt
    12421 ttgccgacaa gggcaaatcg caccacacca caactttcgg tctgctgctg ctgttacttt
    12481 catggctggc aatctggttt ggtcacactt ccgtactggc gttgattatc ggcattctgg
    12541 tgctggacct caccgtacag ggcgtgcata tcactaacca gacggtaatt tatcgaatac
    12601 atcctgatgc gcgtaatcgc ctgaccgcag gttacatgac cagctacttt attggcggtg
    12661 ccgccggttc gctaatttca gcctcagcct ggcaacatgg cggttgggct ggcgtttgtc
    12721 tggctggcgc gacgattgcc ctggttaact tactggtctg gtggcgaggt tttcatcgtc
    12781 aggaagccgc aaattaagca atcagcaacc gttttcgtgg ctttacactt ataagggtgt
    12841 taagaagccc atcagtctga taaggttaag atattcattc agtctattta taatattaac
    12901 aatcgttaag cgtacactct atggaaagcc ctactacaca gcctgctcct ggttcggcga
    12961 ccttcatgga aggatgcaaa gacagtttac cgattgttat tagttatatt ccggtggcct
    13021 ttgcgttcgg tctgaatgcg acccgtctgg gattctctcc tctcgaaagc gtttttttct
    13081 cctgcatcat ttatgcaggc gcgagccagt tcgtcattac cgcgatgctg gcagccggga
    13141 gtagtttgtg ggttgctgca ctgaccgtca tggcaatgga tgttcgccat gtgttgtatg
    13201 gcccgtcact gcgtagccgt attattcagc gtctgcaaaa atcgaaaacc gccctgtggg
    13261 cgtttggcct gacggatgag gtttttgccg ccgcaaccgc aaaactggta cgcaataatc
    13321 gccgctggag cgagaactgg atgatcggca ttgccttcag ttcatggtca tcgtgggtat
    13381 ttggtacggt aataggggca ttctccggca gcggcttgct gcaaggttat cccgccgttg
    13441 aagcagcatt aggttttatg cttccggcac tctttatgag tttcctgctc gcctctttcc
    13501 agcgcaaaca atctctttgc gttaccgcag cgttagttgg tgcccttgca ggcgtaacgc
    13561 tattttctat tcccgtcgcc attctggcag gcattgtctg tggctgcctc actgcgttaa
    13621 tccaggcatt ctggcaagga gcgcccgatg agctatgagg ttctgctgct tgggttactg
    13681 gttggcgcgg cgaattattg cttccgctat ttgccgctgc gcctgcgtgt gggtaatgcc
    13741 cgcccaacca aacgtggcgc ggtaggtatt ttgctcgaca ccattggcat cgcctcgata
    13801 tgcgctctgc tggttgtctc taccgcacca gaagtgatgc acgatacacg ccgtttcgtg
    13861 cccacgctgg tcggcttcgc ggtactgggt gccagtttct ataaaacacg cagcattatc
    13921 atcccaacac tgcttagtgc gctggcctat gggctcgcct ggaaagtgat ggcgattata
    13981 taacccacaa gaatcatttt tctaaaacaa tacatttact ttatttgtca ctgtcgttac
    14041 tatatcggct gaaattaatg aggtcatacc caaatggata gttcgtttac gcccattgaa
    14101 caaatgctaa aatttcgcgc cagccgccac gaagattttc cttatcagga gatccttctg
    14161 actcgtcttt gcatgcacat gcaaagcaag ctgctggaga accgcaataa aatgctgaag
    14221 gctcagggga ttaacgagac gttgtttatg gcgttgatta cgctggagtc tcaggaaaac
    14281 cacagtattc agccttctga attaagttgt gctcttggat catcccgtac caacgcgacg
    14341 cgtattgccg atgaactgga aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat
    14401 cgccgctgcc tgcatctgca attaacggaa aaaggtcacg agtttttgcg cgaggtttta
    14461 ccaccgcagc ataactgcct gcatcaactc tggtccgcgc tcagcacaac agaaaaagat
    14521 cagctcgagc aaatcacccg caaattgctc tcccgtctcg accagatgga acaagacggt
    14581 gtggttctcg aagcgatgag ctaacgcgtc atctcgctca aaaatccaga tttataaaag
    14641 aaaaaatgac cggccagcat cgcaacatgc tggc
//
LOCUS       DAZGCQ010000113        14521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000113 DAZGCQ010000000
VERSION     DAZGCQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.113"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            268..474
                     /locus_tag="ACHK07_004037"
     CDS             268..474
                     /locus_tag="ACHK07_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104309.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            1027..1521
                     /locus_tag="ACHK07_004038"
     CDS             1027..1521
                     /locus_tag="ACHK07_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO8104310.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1521..3623
                     /locus_tag="ACHK07_004039"
     CDS             1521..3623
                     /locus_tag="ACHK07_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8104311.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSHSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3620..3832
                     /locus_tag="ACHK07_004040"
     CDS             3620..3832
                     /locus_tag="ACHK07_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104312.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3832..5340
                     /locus_tag="ACHK07_004041"
     CDS             3832..5340
                     /locus_tag="ACHK07_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8104313.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFVQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5285..7312
                     /locus_tag="ACHK07_004042"
     CDS             5285..7312
                     /locus_tag="ACHK07_004042"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO8104314.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7399..7722
                     /locus_tag="ACHK07_004043"
     CDS             7399..7722
                     /locus_tag="ACHK07_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO8104315.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7715..7990
                     /locus_tag="ACHK07_004044"
     CDS             7715..7990
                     /locus_tag="ACHK07_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO8104316.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            8002..8580
                     /locus_tag="ACHK07_004045"
     CDS             8002..8580
                     /locus_tag="ACHK07_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104317.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLVNGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8577..8978
                     /locus_tag="ACHK07_004046"
     CDS             8577..8978
                     /locus_tag="ACHK07_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8104318.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8989..9732
                     /locus_tag="ACHK07_004047"
     CDS             8989..9732
                     /locus_tag="ACHK07_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104319.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEICAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASINVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            9793..10179
                     /locus_tag="ACHK07_004048"
     CDS             9793..10179
                     /locus_tag="ACHK07_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8104320.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELSEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            10188..10517
                     /locus_tag="ACHK07_004049"
     CDS             10188..10517
                     /locus_tag="ACHK07_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8104321.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10489..13554
                     /locus_tag="ACHK07_004050"
     CDS             10489..13554
                     /locus_tag="ACHK07_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8104322.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKSAQAHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSSGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSYMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13554..13883
                     /locus_tag="ACHK07_004051"
     CDS             13554..13883
                     /locus_tag="ACHK07_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104323.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13893..>14521
                     /locus_tag="ACHK07_004052"
     CDS             13893..>14521
                     /locus_tag="ACHK07_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8104324.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSTSFVLSTPTETDG
                     SVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCL"
BASE COUNT         3557 a         3353 c         4338 g         3273 t
ORIGIN      
        1 catcagatgc tattcaacaa tacaaattac ccataaacct cgtttttacg gggttttgtt
       61 atatttaaac attaccgaat agatataaat ctcgattgtc gatggtattg gttgtgtgac
      121 aatacctagt ctttccggta tgcctgaaga gaatacaaac gacagattat gtaagggaaa
      181 cgcgctgaaa ctttcactct ttcatgctga gtgatttgac gttatatttt tagccgcaag
      241 tgaagagcaa acgcatggag cgacaaaatg cataaagatc aatacactga tgccccctcc
      301 caggagcagg ttcgtgtaaa aactatgctt aatagcacca tttcgatggg ttacccggat
      361 gttgtaattg catgtataga acatcaagta tctctggaag cattcagggc aattgaggcc
      421 gcgttagtaa agcacgataa gaatacgaag gattattccc tggtggttga ctgatcacca
      481 taactgctaa ccattctgac catttcacaa gtgacagagt caatatcgta ttctgtcact
      541 gtcaggctaa tacagtactg caattcaact acagcaatgc ctcgtattta ggtgaattta
      601 caatatcgtc cagttcggat gccggctgca tttctgaaga taaggcattt cggttcgcgt
      661 attttcccct catgctcgtc agtcctgcgc gtagtaagaa acaggacact cacactaatt
      721 tgtgtgggca tgctgtgatg tccttctgaa ttatccccac gccattatgt aaagctctgt
      781 ttcagatgct cgtcacggtt gtcaggctgt cgggtcctcc ctgtgggggg ccctgccacg
      841 gggcgggagc gtcgcggaaa aaggctagtt tttgaaattt cattcgtcat caccaccact
      901 gtaatagatt gatattacag tggttttatt tttatggtgt cgattttgat tgttttttgt
      961 tcatcactaa caccgtttgc ctaaagttgt tcgcaagatg catgtttaaa acattctgga
     1021 gcgggtatgg atcgagagtt aaaaaatctg acgctgaata tcagtcaact ggcggcactg
     1081 tcaggtgtac atcgccagac tgctgcggca aggctgcaaa atctacccgt tgcagggggg
     1141 catgaaagca acctcaagct ttatcgggtg gttgatattg tgtcggcatt tctggcatta
     1201 ccaccgccgg ttgcagaagg cgaaatggac gcgcatgagc gcaaagcctg gtatcagtct
     1261 gaacgtgagc gtcttaagtt cgaacaggaa acggcacaac tcattccggc cagtgatgtc
     1321 agacgggagt ttgccatctg ggcaaaagcg gtcgtgcagg tgctggagac attaccggat
     1381 attcttgaac gtgactgcgg tctgcagcct gccgctgtga gccgtgttca gtccattatt
     1441 gatgatctgc gcgatcagat agccctgcgg gtgactgaag caggtgcgga tgatgaggag
     1501 gaattacagc aggaggagta atgctgaatc aggaaaccgc aaaggcagca cgaaccgatt
     1561 caggttatat ccttcgcgca ccgagacgaa tgcgggttgc tgatgccgtt gctcagtata
     1621 tgcgggtgcc catgggggca gggaactcag tcccgtggga tccgctggtg gcaccgtatg
     1681 ttattgagcc gatgaactgc ctggcctcgc gtgaatacga cgcagtgata tttgttggcc
     1741 cggcacgaac cggcaagact atcggcctga ttgacggctg ggtgatttac aacgtgattt
     1801 gcgatcctgc tgatatgctg atcattcaga tgacggagga aaaagcccgc gaacactcca
     1861 aaaaacgact cgccagaacg tttcgcgtca gcccggaagt ggtcagtcgc ctgagtccga
     1921 acaaaaatga caacaacgtt tatgacagaa cattccttgc tggtaactac ctgaaaatcg
     1981 gctggccgtc agtcaatatc atgtcctcat cagattataa atgcgtcgcg ctgacggatt
     2041 atgaccgttt tccggaagat attgatggcg agggggatgc tttctctctt gcctcaaaac
     2101 gtaccacaac atttatgtcc agtggtatga cgcttgtgga gagttccccc ggcagggatg
     2161 tgaaggatgt gaaatggcga cggacttcac cgcatgaggc tccaccaacc acggggatac
     2221 tgtcgctcta taaccgtggc gatcgccgtc gctggtactg gccctgtcca cactgtggtg
     2281 agtattttca gccctgcggc gatgtggttg ctggtttccg tgatattgcc gatcccgtgc
     2341 tggcaagtga ggcggcttat attcagtgtc cttcctgttc aggacggatt atgcctgaac
     2401 aaaaacgtga gctgaacgga cgtggggtct ggttgcggga tggtgaatcc atcaatgcgg
     2461 atggcagtcg ttatggtgat ccccgacgct cacgtattgc gtcattctgg atggagggtc
     2521 cggcagctgc ttaccagaca ctctcgcaac tcgtttacaa actgcttact gcagaacagg
     2581 aatacgagac aaccggaagt gaagaaacac tcaagacggt tatcaatacc gactggggat
     2641 taccttatct tccccgcgcc agcatggagc aacgaaaaag tgaactgctt gagcagcggg
     2701 cagagccagt tccttcccac agtgtgccgg atggcgttaa tttccttgtg gcgacagtgg
     2761 atgtgcaggc gggacgtcat cgccgttttg tggttcaggt aacgggctat ggcagccgtg
     2821 gcgaacgctg gattattgat cgttacaaca tcacgcagtc attgcgcggt gacagcgacg
     2881 gggagagcca gcgaattgat ccggccagct atccggaaga ctgggatgtc ctgctgacgg
     2941 atgtttttca taaaagctgg ccgctggcct ccgatccttc tcaacaaatg cgactgatgg
     3001 caatggcggt ggactccggc ggtgaagacg gggtcactga taatgcctat aaattctggc
     3061 gtcgttgccg tcgtgatggc cttggtaaac gtatttacct gtttaagggc gacagcatcc
     3121 ggcgcgcaaa actgatcact cgtacattcc ctgataacac cggacgaacg ggccgacggg
     3181 cgcaggccgc aggtgatgtg ccgctctggc ttcttcagac ggatgcactg aaagaccggg
     3241 tgaataacgc gttatggcgt gactcgccag gtcccggcta tgtgcatttc cctgactggc
     3301 tggggagctg gttttacgac gaactgacgt atgaagagcg gagcagtgac gggaaatgga
     3361 gtaagcctgg tcgcggtgcc aacgaagctt ttgacctgat ggtgtatgcc gaggctctgg
     3421 tcattctgca tggatacgaa aagatccgct ggccggatgc accggagtgg gcgagccggg
     3481 aaacctggct ggagtgtgtc caggacagta ccgaaccgtc atcctcaccg gaaccggtat
     3541 ccacgcctgt taaaaaacaa aaacggaaga aaacagtaac tgacgatgtt aacccctggc
     3601 tgacttccgg aggatggtta tgaaccagaa tgatatcgaa gccatgattc agcgttatat
     3661 ggaagctgaa atggcggtgc tggacggaaa atccgtcacc tttaatggtc agcagatgac
     3721 catggaaaac ttatctgaga tccggcaggg acggcaggag tgggagcgcc gccttgcggc
     3781 tctgattaca cgacgacggg ggcatcccgg gtaccggctg gcgaggttct gatggcaatt
     3841 cttgatgatg tgattggcgt tttttcacct ggatggaaag cggcaaggct gcgttcccgt
     3901 gcggtgatcc aggcttatga agccgtaaaa acgacgcgga cacacaaagc ccgacgggag
     3961 aaccgaactg ccgaccagct aagccagtac ggggccgtgt cgttacgtga gcaggcccgt
     4021 taccttgata acaaccacga tctggttatt ggtgtatttg acaagctgga agaacgggtg
     4081 gtggggaaaa acgggattat tgtcgagcca catccggtat tacgcaatgg ggccattgcc
     4141 cgtgatctgg cagcggagat tcgcacccga tggagtgaat ggtctgtcag cccggaagtc
     4201 accgggcagt ttacccgtcc gatgctggaa cgtctgatgc tgcgtacctg gctgcgcgat
     4261 ggtgaggtgt ttgtccagat ggtttccggg cgcataaaca gcctgacgcc ttctgccggt
     4321 gttcatttct ggctggaggc gctcgagcca gactttattc ccatgaccag tgatgagagc
     4381 aacaggctga atcagggcgt gtttgttgat gactgggggc gtcccgaaaa atatctggtg
     4441 tataaaagcc gtcccgtatc cggacggcag atggaaacca aagaagtgga tgcagagcga
     4501 atgctgcatc ttaaatttgt tcgccgtctg caccagatgc gcgggacgtc tttgttgtcc
     4561 ggtgtgctga tccgcctcag tgccctgaaa gattatgaag attatgagct gactgcagca
     4621 aggatcgccg ctgctctggg gatgtacatc cgcaaaggcg acgggcagag ctatgaaccg
     4681 gatggtaatg gcagcaagga taaggaacgc gagcttacca ttcagccagg cattatttac
     4741 gacgatctga aacccggcga agaaatcgga atggtgaagt cggatcgccc caatcctaac
     4801 cttgaaactt ttcgtaatgg tcagttgcgt gccgtggcgg cgggcagtcg tctgagtttt
     4861 tccagtacag cgcgcaacta taacggcact tacagcgccc agcgtcagga gctggttgaa
     4921 tctactgatg gctacctgat cctgcaggac tggtttattg gtgccgtcac ccgcccgatg
     4981 tatcgtgcat ggctgaaaca ggctgtggca tccggtgtta tcaggctacc ccgcgatctt
     5041 gaccgttctt cactgtatac cgcggtgtat tccggaccag tgatgccgtg gattgaccct
     5101 gttaaggagg ctgaggcctg gaaaatccag attcgtggtg gagcggcgac agaatcagac
     5161 tgggtacgtg ctggtggtcg taatccggat gatgtcaaac gtcggcgcaa ggccgaaatt
     5221 gatgaaaacc gcaagctgga tctggtattt gataccgatc cggccagtga taaaggaggc
     5281 agcagtgccg caacgaaacg acaggagccg cagcacaccg acgaccagtc cgaagaataa
     5341 ttcctggttc aggatgcagg ctggtcacca gagtgacgcg gatatttata tttatgacga
     5401 gattggtttc tggggtgtta cagcgaagca gtttatcagt gatctgaatg cactgggcga
     5461 tatcacccac attaatctcc atattaattc accgggtggc gatgtctttg aaggcatcgc
     5521 catttttaat gcgctgaaaa cacatggtgc gtccattacc gtttatgtcg acggtgtggc
     5581 ggcgtcaatg gcgtcggtca ttgcgatggt gggaaacccg gtcattatgc cggaaaacac
     5641 cttcatgatg attcataaac catttggctt tacgggcggt gatgcggagg acatgcgcac
     5701 ctatgccgac ctgctcgata aggttgaggc ggttctgtta cccgcttatg cacagaaaac
     5761 cgggaaaacc accgatgaaa ttgctgccat gctggcggat gagacctgga tgtccggtgc
     5821 cgaatgtctg gcacagggat ttgctgatca ggtgacgcca gccgttaagg caatggcatg
     5881 tattcagtca aaacgtacag aggaatttaa aaagatgccg gaatccattc gaaacatgat
     5941 tactccgcca cgcaacagtg ctccacgcgt acaggatgat gaacctgcag cctcccggac
     6001 gccagtgcag gcagcagcac ccgtggtgga tgaaaacagt atccgtgcgc aggtactggc
     6061 agagcaaaaa gcgcgtgtaa acggtattaa tgatctgttt gccatgtttg gcgggcgtta
     6121 tcagacgctg caggctcagt gtcttgccga tcctgaatgt tcgctggagc aggcccgcga
     6181 aaagctgttg aacgagatgg ggcgcgagtc cacgccatcc aataaaaata ccccggctca
     6241 tatttatgcc ggaaacggta attttgtggg ggacgggatc cgccaggcgc tgatggcgcg
     6301 tgccggattt gaaaaaaccg aacgtgataa tgtctacaac gggatgaccc tgcgtgaata
     6361 tgcccgtatg tcactgactg aacggggtat tggggtttcc agttataacc cgatgcaaat
     6421 ggtcggtgcg gcgttcacac acagtacgtc tgacttcggt aatattctgc tggatgttgc
     6481 gaacaaagcc attctgcagg gctgggaaga tgcccctgaa acctatgaac agtggacgcg
     6541 gaaaggtcag ttgtctgatt ttaaaattgc ccatcgtgtg ggtatggggg gcttcagtgc
     6601 tctgcgtcag gtgcgtgaag gggcggaata taaatacgtc accaccggag ataaacaggc
     6661 cactattgca ctggcgacct atggcgagct gttcagtatc acccgtcagg ccattatcaa
     6721 tgatgatctg aatatgctga ccgatgtccc gatgaaactg ggccgtgcgg cgaaatccac
     6781 tattgccgat ctggtttatg ccattctgac gtctaacccg aaaatctcca cagataatgt
     6841 aagtctgttc gataaagcga aacatgcaaa cgtactggag agcgctgcaa tggacgtggc
     6901 atcgctggat aaagcccgcc agttgatgcg cgttcagaaa gagggggagc gtcatctgaa
     6961 tattcgtcct gcgttcgtac tggtaccgac ggcgatggag tctgttgcta accaggtcat
     7021 tcgctcctca agtgtcaagg gggctgacat taacgccggt attattaacc cggtgaaaga
     7081 ttttgcgacc gttattgcag agcctcgtct tgatgataac agccagacca ccttctacct
     7141 ggctgcgtca aaaggctccg atacgattga agtggcttat ctcaacggtg tggatacgcc
     7201 atatattgat cagatggagg gcttcagtgt ggatggcgtg acaacgaaag tgcgtattga
     7261 cgccggtgtc gcgccagttg atcaccgcgg tctggtgaaa tgtacggcgt aaacgtcgca
     7321 gacaacaact ctgatggccc gtaagggctt tttttgtacc tgaaatcagc ccctgaacga
     7381 ggctgtgcgg agacagttat ggcaaagaat tttgtagaag aaggaaaaac ggtggcgatt
     7441 gttgccagtg cagccatcag cagcggagac ctggtgcagg tgggtgatgt ttttgcggtg
     7501 gcgctgaccg atattccaca gggtgaaaca ggcgacggcc tgaccgaagg tgtgtttatg
     7561 ctgcctaagc tgaaaacgga tgacatgaaa acgggtaaga aggtttatct gaagtccgga
     7621 aaagttcagt tgactaacag cggctctgat ccgctggtcg gggttgtctg ggcagatgcc
     7681 ggaaccagtg cagaagaagt gccggtaaaa ctcaatgtct gatccctttt cccggctggc
     7741 agcgcgtatg gatgcgatca cggtcagaaa gatgggaaag acagcctcga ttaatgatgt
     7801 cgatatgact gtgatcccgg gagaaacact ggcagagctg aatgctctgt ccggacctgc
     7861 ggtctctctg gtggtgtttt cttcgggata ccgcccacgg cgcggggatc gcgttgttta
     7921 tgacggacaa cattggacgg tcacacggca tgaacgcttt aacggtaagc caatgatctt
     7981 tattgagtaa agaggtgtgg gatgaagggg cttgagaatg ccatccgcaa tctgaacagc
     8041 cttgataccc gtatggtgcc acaggccagc gcatgggcga taaaccgtgt ggcacagaaa
     8101 gcggtctcgg ttgccacccg gcaggttgcc gggaataccg ttgcgggaga taaccaggtg
     8161 aaagggatac ccctgaaact ggtacgtcag cgtgtccggg tgtttaaagc cagtccgtca
     8221 ggaaaaatga cggccaggat ccgcgttaac cggggcaatc tgcctgccat taagctgggg
     8281 accgcccggg tcagactgac ccggcgtggt ggaaaactgc agtaccgtgg cagcgtgctg
     8341 aaggtgggta aatatctttt ccgggatgcg tttattcagc aactggtgaa tggtcgctgg
     8401 catgtgatgc ggcgtattga tggcaaaaat cgttacccca ttgatgtggt gaaaatccct
     8461 ctgtccggac cgctgacaca ggcatttgaa gatgcccgcg accgcatcat tgctgcggaa
     8521 atgccgaaac agctggggta tgcactgaaa caacaactga ggttatggct gacccgatga
     8581 accgacatac acaaatccgc caggtcgtac tggcacgcct tcgggaacag tgtggagaca
     8641 gcgccacgtt ttttgacgga cttccggcat ttgttgatgc gcaggaactg cctgccgtgg
     8701 cggtgtggct gagtgatgct cagtacaccg gaaaaatgac ggatgaagat gactggcagg
     8761 ctgttctgca tattgctgtc ttcatccggg cacaggcacc ggattcagag ctggatatgt
     8821 ggatggagag caccattttc ccggccctga atgatgtacc ggcactttcc ggactcatcg
     8881 acaccctgat cccactcggt tttaactatc aacgtgataa tgagatggcc acctgggcga
     8941 tggcggaaat cacgtaccag atcacgtaca cgaattaagg aggtggtaat gaccacacca
     9001 aatccactgg caaaaacgaa aggtgcggga acgacgttct ggatgtacac cggcaagggc
     9061 gatgcgtttg cgaacccttt atcggacact gactggctgc gtcttgcgat ggtgaaggat
     9121 ctgcaacctg gcgaaatgac cgctgatgca gaagatgaca cttatctcga tgatgaagat
     9181 gcagactgga aaacgacaac tcaggggcag aaatccgtcg gtgatacttc ggcgacgctg
     9241 gcctggcgtc cgggtgacag cggacagaaa aaactggttc agttgttcga ctccggtgaa
     9301 atctgcgcgt ttcgtatcaa atatcccaac ggcactgttg atgttttccg tggctggctg
     9361 agttcactgg gtaaaaccat tgcctcaaaa gacgtgatga cccgcaccgt gaaaatcagc
     9421 ggtgtggggc gtccgtatct ggcagaggag ggcactgaaa ccgtgggcgt taccgggctg
     9481 acggtggcac cggcatccgc cagtatcaat gtgggagcaa ccaccacgct gacctttaca
     9541 gtaaaacctg acggagccag tgacaaagcg atcagtgtgc attcgacaga tccacagaca
     9601 gccactgtga ccctgaacgg acttgtggcc acggtgaaag gcgtgaagca gggcagtgtc
     9661 agcattgtgg gcatgacttc tgacggcgat tttgtggcag tggctacggt ggctgtcagc
     9721 gccgcaggtt aacaggacga tactcatcat ttgccccggt tattcggggc ttttttgcag
     9781 gtggagaata tgatgtttct gaaacagggc acgtttaatt atgaaaaaca gtccgtggtg
     9841 ctcagtgagc tgtccgggct gcagagaatt gaatatctgg cgtttgttca gcagcgaacg
     9901 gcaaagtttg atgccgggga gggagaactg tcggaggctg aacgacagat tgcttttctg
     9961 cggatgggga tggatatcaa tgcctggctg gtttcccgat cactgtggaa tgcggatcag
    10021 tctaaggatg tagagacgct ttgcgcatcc gttattacaa catggtcgta tgatgccctg
    10081 ggggcggggg cggagatggt tctgtcgctg agcggtatgg gggccattga taatgccggg
    10141 gatgatgagc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg cagtttgtca
    10201 tgcggcttgc ccgggagttc cggcgggcag actggcggcg gatgctgtcg gaaatgtcgg
    10261 ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt gatgtgtgga
    10321 tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc ggcagcagtg
    10381 atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg gagcgaacgg
    10441 acgaagaact gatgcatctt ggagaaggta tttccggagg tgtgcgttat ggaccagata
    10501 gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga aattccccgt
    10561 atcaaaaacc ttctgaatgg tgccgccagc gatgcagaac ggtcttctgc ccgtatgcag
    10621 cgttttatgg agcgtcagac tcaggccgcc cggcagacaa cgcaggcggc ttcttcggct
    10681 gcaacagccg cctccgtcca tgcgcagacg gtggagaaga gcgcacaggc tcatgaacgc
    10741 atggcccgcg aggtggagaa aacccgccag cgcatggagg cactgagcca gaaaatgcgc
    10801 gatgaacagg cgcaggccat ggctctggcg gaggctcagg ataaagcggc tgctgcgttt
    10861 tatcgtcaga ttgacagtgt gaaacaggcc agtgcggggc tgcaggaatt acagcgtatt
    10921 cagcagcaga tccgacaggc cagaaacagt ggcgggattg gtcagcagga ttatctggcg
    10981 ctgatttctg aggttacggc gaaaacccgt gttcttacac aggctgagga agaggctacc
    11041 cgacagaaag tggcgtttat ccgtcagctt aaagagcagg caacccgcca gaatctttct
    11101 tcttctgagt tgcttcgtgc caaggctgcc cagctggggg taagcagtgc tgcagaagtg
    11161 tatatccgca aaatggagca ggcaggaaaa gccacgcatt cgctgggtct gaaaagtgca
    11221 gcggcccgcc aggagatagg cgttctgata ggtgaactgg ctcgcggcaa tttaggtgcg
    11281 ctgaggggat ccgggataac gctggctaac cgtgccggat ggatagacac actgatgtca
    11341 ccgaaaggca tgatgctggg cggggttatt ggcggtattg ccgcggccgt ctatggtctg
    11401 ggtaaagcct ggtatgacgg tcagaaggag ggggaagaat ttaaccgcca gttgtcgctg
    11461 acggggcatt atgccggagt cactgccggg cagctgtgga cgcttagtcg tgctatttcc
    11521 gggaatggta tcacgcaaca tgctgcagcc ggtgcgctgg ctcaggtggt ggggagtggt
    11581 gcatttcgtg gaaacgatat cggtatggtg gcgagagctg ccgcacagat ggagcgatcg
    11641 gttggccagt cggtcagcga taccataaat cagtttaagc ggctgaagga tgatcctgta
    11701 aatgccgcga aggctctgga caatgagctg cattttctta ctgccactca gcttgagcag
    11761 atacgtgtcc ttggggatca gggacggtcc agtgatgctg cacggatagc catgtctgca
    11821 ctggcagagg aaaccggtcg gcgtactgcg gatattgata ataacctcaa tgcgctgggc
    11881 agtacgctga agtatctgtc tgatttatgg agtcgtttct gggatgcggc catgaatatt
    11941 ggtcgtgaag actcgctgga tgaacagatt tccgctttac aggagaaagt gtcgcgggcg
    12001 aaaagactcc cctggacggc atcatcttct caggttgaat acgatcagca gcgtcttaac
    12061 gagcttcagg agaaaaaacg ccagaaggat ttgcaggatg caaaagagca ggcagagcgg
    12121 aattatcagg agcaacagaa acgccgtaat gctgaaaatg ctgcactgaa ccggatgaat
    12181 gaaacggaag cagcacgaca tcagcgtgaa attgcgcgta ttaatgccat gcagtacgcc
    12241 gatcaggctg tcagggatgc ggcgatacaa cgtgaaaatg aacgttacga gaaagccctg
    12301 gcatccggta agaaaaaaac acgcgaaacc cgtaatgatg aggccacccg gttattgctg
    12361 cagtacagtc agcaacaggc acaggtggaa ggacagattg ctgctgccag acagtcatca
    12421 ggcattgcca cggaaaggat gacagaagct cataaacagc ttctggctct gcagcagcgc
    12481 atcagcgacc tggacgggaa aaaactgacg gcagatgaaa agagtgtgct ggcccgtaaa
    12541 gatgaactga ttcaggcact gacgctgctg gatgtaaaac agcaggagct tcagaaacag
    12601 acggcactca acgagctgaa gaaaaaaaca attcagctga ccagtcaact ggctgaagaa
    12661 gagcgcgctc agcgtcagca acatgacctg gatattgcca cggtgggtat gggtgatcag
    12721 cagcggcagc gatatcaggt acaactgagt cttcgccaga aataccagca acagctggag
    12781 cagttgaggc gggatagtga gcagaaagga acatataaca cggatgacta cagaaaggcc
    12841 gagcaggcgc tgacggagag cctgaaccga caactgaatg agaatcgccg ttactggcaa
    12901 cagcttgaag ttgtgcaggg taactggaaa aacggagtcc tgcgtgcatt tcaggatttt
    12961 accgtggatg cagataatac ggcaggaaca gcagaacagg tgttctcgtc agccttcagc
    13021 tacatgggaa atggcctggc aacttttgtc actaccggca aactcaattt caaatccttc
    13081 acctcttctg tgctgtcaga tatggcgaaa atcctggcgc aggcaaccat gatgaaatcg
    13141 ataaaaggga ttggcagtgt actgggattt gatctcagca gcctttccct gaatgccaat
    13201 ggggggattt atcagtctgc tgatttgagt cgttacagtg gcacggtggt taaccgtccg
    13261 acgttttttg cttttgcaaa aggcgcgggt gtgatggggg aagcgggacc tgaagccatt
    13321 ctgcctctgc gtcgtggtgc tgacggaaag ctgggggttg tggcggatat tggtggttca
    13381 ggtatggcga tgtttgcccc gcagtacaac atcgagatca ataacgatgg cacgaacggg
    13441 cagataggtc cggctgccct gaaggtggtt tatgaccttg ggaaaaaagc ggcagcggac
    13501 tttatgcaac agcaggcccg tgatggtggt cggttaagtg gagcatatcg gtaatggaga
    13561 cgtttcactg gaaagtgcgc ccggatatga atgtggtatc agagccgaaa gtggtgacag
    13621 tgaagctggg cgatggttat gaacagcgtc gtgcggcggg actgaataac cagttgtcga
    13681 cttacagcgt gacgatacgt gttcgtaaat gtgaacaccc atctttaaaa gcctttctgg
    13741 aacggcacgg tggcgtccgc gcatttcagt ggacgccacc ttatgactgg aaaccgatca
    13801 gggtggtttg tcgcaaatgg tcggcaagcg tgggggcgct gtgggtaacc ataacggcag
    13861 attttgaaca ggtcgtggca taggaggctc tgatgcagga tattccacag gaaacacatc
    13921 atgagacgac acgcctcact cagtcagccc aggtggtgct ctgggaaatc gatctgacag
    13981 aggtcggtgg tgaacgttat tttttctgta atgagcagaa cgaaaaaggt gagccggtta
    14041 cctggcaggg gcggcagtat caggcatacc ccattcaggg gacgggattt gaactgaatg
    14101 gcaagggcag tgctgcccgt ccgacactga cggtttctaa cctgcacggc atggtcaccg
    14161 ggatggcgga agacctgcag agtctggtcg gcggaacggt ggtcaggcgt aaggtttacg
    14221 cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat ccggagcagg
    14281 aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagtgcggtc agtacctcct
    14341 ttgtactgtc cacaccgact gaaacggatg gctctgtttt tccggggcgt atcatgctgg
    14401 ccaacacctg cacctggacc tatcgcggcg atgagtgcgg ttatagcggt ccggcggtcg
    14461 cggatgaata tgaccagccg acgtccgata tcacgaagga taaatgcagc aaatgcctga
    14521 g
//
LOCUS       DAZGCQ010000114        14502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000114 DAZGCQ010000000
VERSION     DAZGCQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.114"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..1793
                     /gene="garD"
                     /locus_tag="ACHK07_004053"
     CDS             222..1793
                     /gene="garD"
                     /locus_tag="ACHK07_004053"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO8104325.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1942..2277
                     /gene="prlF"
                     /locus_tag="ACHK07_004054"
     CDS             1942..2277
                     /gene="prlF"
                     /locus_tag="ACHK07_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO8104326.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2277..2741
                     /gene="yhaV"
                     /locus_tag="ACHK07_004055"
     CDS             2277..2741
                     /gene="yhaV"
                     /locus_tag="ACHK07_004055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO8104327.1"
                     /translation="MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2796..3605)
                     /gene="agaR"
                     /locus_tag="ACHK07_004056"
     CDS             complement(2796..3605)
                     /gene="agaR"
                     /locus_tag="ACHK07_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO8104328.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRLQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3854..5134
                     /gene="kbaZ"
                     /locus_tag="ACHK07_004057"
     CDS             3854..5134
                     /gene="kbaZ"
                     /locus_tag="ACHK07_004057"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO8104329.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPV
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5157..5630
                     /gene="agaV"
                     /locus_tag="ACHK07_004058"
     CDS             5157..5630
                     /gene="agaV"
                     /locus_tag="ACHK07_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO8104330.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5641..6420
                     /gene="agaW"
                     /locus_tag="ACHK07_004059"
     CDS             5641..6420
                     /gene="agaW"
                     /locus_tag="ACHK07_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO8104331.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6410..7288
                     /gene="agaE"
                     /locus_tag="ACHK07_004060"
     CDS             6410..7288
                     /gene="agaE"
                     /locus_tag="ACHK07_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO8104332.1"
                     /translation="MASNQTTLPNISENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTMTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7306..7740
                     /gene="agaF"
                     /locus_tag="ACHK07_004061"
     CDS             7306..7740
                     /gene="agaF"
                     /locus_tag="ACHK07_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO8104333.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7737..8870
                     /gene="nagA"
                     /locus_tag="ACHK07_004062"
     CDS             7737..8870
                     /gene="nagA"
                     /locus_tag="ACHK07_004062"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8104334.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRYLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQGQLASF"
     gene            9221..10375
                     /locus_tag="ACHK07_004063"
     CDS             9221..10375
                     /locus_tag="ACHK07_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO8104335.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10388..11248
                     /gene="kbaY"
                     /locus_tag="ACHK07_004064"
     CDS             10388..11248
                     /gene="kbaY"
                     /locus_tag="ACHK07_004064"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO8104336.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11415..11891
                     /gene="agaB"
                     /locus_tag="ACHK07_004065"
     CDS             11415..11891
                     /gene="agaB"
                     /locus_tag="ACHK07_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO8104337.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11930..12733
                     /gene="agaC"
                     /locus_tag="ACHK07_004066"
     CDS             11930..12733
                     /gene="agaC"
                     /locus_tag="ACHK07_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO8104338.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12723..13514
                     /gene="agaD"
                     /locus_tag="ACHK07_004067"
     CDS             12723..13514
                     /gene="agaD"
                     /locus_tag="ACHK07_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO8104339.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13515..14270
                     /locus_tag="ACHK07_004068"
     CDS             13515..14270
                     /locus_tag="ACHK07_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO8104340.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQHVDVSQLTVVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         3529 a         3648 c         3756 g         3569 t
ORIGIN      
        1 tatttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg
       61 ctttaccttg ccgggcggca ttgcaatccc tgaaatgatt gacattgatc acatttctgc
      121 atttaaactc ctgacattct tatttcaccc aatgaagtca tttattttta aatgagacca
      181 ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca
      241 gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta
      301 ttgttaatga taatggcctg aaagcgggaa cgcgttttcc ggatgggctg gaattaattg
      361 aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta
      421 ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg
      481 acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca
      541 aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg
      601 atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag
      661 gcgtggtgga ctacgtagta aaaatcattg aacgcgatct gctgccgaaa tacccgaacg
      721 tcgatggcgt ggtggggctg aatcatctgt acggttgtgg cgtggcgatt aacgctccgg
      781 cggcagttgt acctatccgt accattcaca atatttcgct gaatcctaac tttggcggcg
      841 aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa
      901 cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa
      961 agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatttac
     1021 aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc
     1081 agtgcggtgg cagcgatgcc ttttctggcg taacggcaaa cccggcggtt gggtatgcgt
     1141 ctgatctact ggtgcgctgc ggcgcaacgg tgatgttctc agaagtcacg gaagtgcgtg
     1201 acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg
     1261 aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc
     1321 cttcgccggg caacaagaaa ggcggtctgg caaatgtagt ggagaaagcg ctcggctcca
     1381 ttgctaaatc gggtaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta
     1441 aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg
     1501 tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga
     1561 tggcggtacc cgtcattaaa atggcgaccc gcaccgagct ggcgaaccgc tggtttgatt
     1621 taatggatat taacgcgggc actatcgcca ccggcgaaga aaccattgaa gaggtgggct
     1681 ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc
     1741 aatggggact gcataaccag ctggcggtgt ttaacccagc accggtgacc tgatttcttt
     1801 tggaacgtcc ctcgcaaaac atggccttag tgccatgttt ttattgttta aagcccccac
     1861 gtccattaat aatgcatttg cattacctta gttcaagctt ataatttgag cagaaaacag
     1921 gctgtaaaag gacagtgaat catgcccgct aatgctcgct ctcacgctgt actgaccact
     1981 gaatcaaagg tcacgatacg cggacaaaca actatccccg cgccagtgcg tgaggcctta
     2041 aaactgaagc caggccagga cagcattcat tacgaaattc tgcctggtgg gcaagtattt
     2101 atgtgccgac tgggagatga acaggaggat catactatga atgcattttt gcgttttctg
     2161 gatgcagata tccagaacaa cccgcaaaaa actcgtccat tcaacattca acaaggaaag
     2221 aaacttgtcg ctggcatgga cgtcaacatt gatgatgaga ttggcgacga cgaataatgg
     2281 attttccaca aagggttaat ggttgggcgc tatatactca tccctgtttt caggaaacct
     2341 acgacgcttt agttgccgaa gtcgagacat taaagggaaa agatcctgaa aattatcaga
     2401 gaaaagccgc cacaaagtta ttggcggtag tccataaagt gattgaggag catatcacgg
     2461 tcaatccatc atcaccggca ttccgtcatg gcaagtcgtt aggctctggg aaaaataaag
     2521 actggtcacg ggtaaaattt ggtgctggtc gttatcgtct cttctttcgt tatagtgaaa
     2581 aagagaaagt catcattctg ggatggatga acgatgaaaa cacgctgcgc acctacggta
     2641 aaaaaacaga tgcctatacc gtattcagca aaatgttaaa aagaggacat cctcctgccg
     2701 actgggaaac cctcacccga gagacagaag aaacccattg atggtgttca caatgcccgc
     2761 cgaacgcatt cgtgttgacc agcgggcacc ctttctcact ccccgaccag aatcacttca
     2821 accccagcct ttcgcagtcc ttccagacta tccgcaggaa tgccttcatc aacaatgatc
     2881 atgtcgatac gttgagtatc aatgatctta tgtaaactgg agcggttgaa cttactggaa
     2941 tcggtgacta cgatgatccg ttccgcaact tcgcacatcc ggcggtttaa acgggcttca
     3001 tcttcattat gcgtgctgac gccgcgctcc agatcaatcg catctacacc aagaaacagc
     3061 atatcgaagt ggtaattttg cagcgattgc tcagcctgat cgccgtaaaa agattgcgac
     3121 tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc cttccgcttc cagcaaagca
     3181 ttagccacgt tcataccgtt ggtcatcgca attacgtcag tgtgcttgcg catcagacga
     3241 gcaatctcaa aagtggtggt cccggaatcg aggatcaccc gatgacctgg ctgaatcaac
     3301 tcaacggcag ctttcgcaac gctgcgtttc attgcggtgt tcagcgcgct tttatcttcc
     3361 actgatggct cgactgacgg cgttgtgcta tcgcagatca acgcgccacc ataggcacgc
     3421 acagcgatcc cctgcttttc cagaaacgcc agatcgttgc ggatcgtcac agtagatacg
     3481 ccatacaatg ccgacagatc gttaacctgc acactccctt gcagtcgcag acgctgaatg
     3541 atctgttctc gtcgctcgct ggtgcctgtc actcgcttct cacctgaagc gtcggtatta
     3601 ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg ttttgcctat taacgccttt
     3661 ctattaagca aatgcaagcc caccttgccc attgacgcaa gctactctcg tttcagtgac
     3721 tttcattatg tttcttttgt gaatcagatc agaaaaccat tatctttcgt tttattttta
     3781 tctcaccatg acgcagtatc aactgaaaca aaacgaaaga ttaatatcgc agtaatctga
     3841 actggagagg aaagtgaaac atctgacaga aatggtgaga cagcacaaag cgggcaaaac
     3901 aaatggaatt tatgccgttt gttccgcaca tccgctggtg ctggaggctg caatccgcta
     3961 cgccagtgca aaccaaacgc cgttactgat tgaagcaacc tccaatcagg tagaccagtt
     4021 cggcggttat accggaatga cgcccgccga ttttcgcggc tttgtttgtc agctcgccga
     4081 ctcgttgaat ttcccgcagg atgcgttgat tctgggtggt gaccatctgg ggccaaaccg
     4141 ctggcaaaac ctgccagtcg ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta
     4201 cgttgcggca ggattcaaaa aaatccacct tgattgcagc atgtcctgtc aggacgatcc
     4261 aattccctta actgatgaca tcgtggctga acgcgccgcc cgtctggcga aagtggcgga
     4321 agaaacctgt cttgaacact ttggcgaagc cgatctggag tatgtcattg gtaccgaagt
     4381 gccggtacct ggcggcgcgc atgaaacctt aagcgagctg gcggtcacca cgccggatgc
     4441 cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat
     4501 ctggccacgc atcattgccc tggtggttca acccggcgtc gaattcgatc acaccaacgt
     4561 tattgattat cagcccgcca aagcgaccgc cttaagccag atggtcgaaa actacgaaac
     4621 gctgattttc gaagcgcact ctaccgatta tcaaacgccg caatcgctgc gccagctggt
     4681 gattgaccac tttgccattc tgaaagttgg cccagcgctg accttcgccc tgcgtgaagc
     4741 tctgttctct ctggcagcga ttgaagaaga actggtgcca gcaaaagcct gttctggtct
     4801 gcgtcaggtg ctggaaaacg tgatgctcga ccgcccggaa tactggcaaa gccactacca
     4861 cggtgacggc aacgcgcgtc gtctggcgcg tggttatagc tactcggatc gcgtgcgcta
     4921 ttactggccg gacagccaga ttgatgacgc tttcgctcat ctggttcgta atctggcgga
     4981 ttcaccaatt ccgctgccgc tgatcagcca gtatctgccg ctgcagtacg tgaaagttcg
     5041 ctccggcgag ctgcagccaa cgccacggga actcattatc aaccatattc aggacatcct
     5101 ggcgcagtac cacacagcct gtgaaggcca ataagcaaaa caaagaggaa cacgctatgc
     5161 caaatattgt tttaagccgg attgatgaac gcttgattca cggtcaggtc ggcgttcagt
     5221 gggtcggatt tgcgggggca aatctggtgc tggtcgccaa cgatgaggtt gccgaagatc
     5281 cggtacaaca aaacctgatg gaaatggtac tggcagaagg gatcgccgta cgtttctgga
     5341 cgctgcaaaa agttatcgac aacattcatc gcgccgccga tcgacagaaa atcctgctgg
     5401 tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca
     5461 ttaacgttgg caatatgcac tacgccaatg gcaagcaaca aatcgccaaa acggtttctg
     5521 tggatgcggg cgatatcgca gcatttaacg acctgaaagc cgctggggtg gaatgcttcg
     5581 ttcagggcgt cccgacagag cctgctgtgg acctctttaa attactttga gggattcatc
     5641 atggaaatca gtctgttgca ggcattcgcg ttgggcatta tcgcctttat cgctggcctg
     5701 gatatgttta acggcttaac ccatatgcac cgcccggtgg tcctcggccc gttagtcggg
     5761 ctggtacttg gcgatctgca taccggtatt ttaaccggcg gtacgctgga actggtgtgg
     5821 atggggctgg ccccgctggc gggcgcacag ccgcctaacg tgattatcgg tactatcgtc
     5881 ggcacggcgt ttgccattac tactggcgtg aaacccgatg tcgcagtcgg tgtcgccgta
     5941 cctttcgctg tcgcagtaca gatggggatt accttcctgt tctcggtgat gtctggcgtg
     6001 atgtctcgct gcgaccggat ggcggagaac gccgatactc gcggcattga acgcgtaaac
     6061 tatctggcac tgctggcact cggcactttc tattttctct gcgctttcct gcctatctac
     6121 ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctac cgcaacgatt aatcgacggc
     6181 ctcggcgtcg cgggcggcat catgccagca atcggttttg ccgtgctgct gaaaatcatg
     6241 atgaaaaacg tctacatccc ctacttcatc ctgggctttg ttgccgcagc ctggctcaag
     6301 ttaccggtgc tggctattgc tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt
     6361 aaatctcctg aaccgactca acctgcggca cagaaagagg aatttgaaga tggcatctaa
     6421 tcaaaccacc ctgccgaaca tcagtgaaaa cgaagaaaca ttgctgactg gcgtcaatga
     6481 aaacgtgtat gaagatcaga gcattggcgc ggagctgacg aaaaaagata tcaatcgcgt
     6541 cgcctggcgt tccatgctgt tacaagcgtc ttttaactac gaacgtatgc aggcttccgg
     6601 ctggttgtac ggtctgctgc ctgctctgaa aaagatccac actaataaac gcgacctggc
     6661 gcgcgccatg aaggggcata tgggtttctt caatacccat ccgtttctgg tgacatttgt
     6721 tatcggcatt atccttgcga tggagcgttc taagcaggac gttaacagta ttcagagcac
     6781 caaaattgcc gtcggtgcgc cgctcggcgg gattggcgat gcaatgttct ggctaacgct
     6841 actgccgatt tgtggcggga ttggagccag tctggcattg caaggctcca ttcttggcgc
     6901 agtcgtcttt attgtgctgt tcaacgtggt acacctgggg ctgcgttttg gtctggcgca
     6961 ttatgcttac cgcatgggcg tggcggcgat tccactcatt aaagcaaata ccaaaaaagt
     7021 cggccatgct gcgtctatcg ttgggatgac ggtaatcggt gcgctggtgg caacctatgt
     7081 tcgtttaagc accacgctgg aaatcacagc gggcgacgca gtggttaagt tacaggctga
     7141 tgttatcgac aaactgatgc ctgccttctt accgttggtc tacaccatga ccatgttttg
     7201 gttggtacgc cgcggctgga gtccgctgcg tctgattgca gttaccgtgg ttctcggcat
     7261 cgtcggtaaa ttctgccatt tcctttaaat acaatgaggt attagatgtt aagtattatt
     7321 ttgacagggc atggcgggtt tgccagcggc atggaaaaag caatgaagca aattctcggt
     7381 gagcaatcgc agtttatcgc catcgatttt ccggaaacct caagcaccgc gctgcttaca
     7441 tcgcagcttg aagaggccat cgcccaactg gattgtgaag acggcattgt ttttctgacg
     7501 gatttgctgg gcggcacgcc gtttcgcgta gcttcaacac tggcgatgca aaaaccgggc
     7561 tgtgaagtga tcaccggcac caatttgcaa ctgctactgg agatggtgct ggagcgcgaa
     7621 gggttaagcg gtgaagaatt ccgtgtgcag gcgctggagt gtggacatcg tgggctgacc
     7681 agtctggtgg acgagttagg ccgctgccat gaggaatgtc cggtcgagga aggaatatga
     7741 cacacgttct gcgcgccaga aggctgctga ctgaagaggg atggctcgat gaccatcagt
     7801 tgcgtattgc tgacggtgtc atcgcagcaa tcgaaccgat tccagtgggc gtgactgaac
     7861 gcgatgcgga actgctctgc cctgcttaca tcgacaccca tgtacacggt ggtgcgggcg
     7921 ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg
     7981 aaggtgtcgg cagttggtta ccgaccaccg taaccgcgcc gcttaatacc attcatgcgg
     8041 cgctgaaacg tattgctcaa cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga
     8101 gttatctcga aggaccgtac ttcacgccgc agaataaagg cgcgcatccg ccggaattgt
     8161 ttcgcgagct tgaaattgcc gagctggatc aattgattgc cgtttctcag cacaccttac
     8221 gcgtggtagc gctggcaccg gaaaaagaag gggcattgca ggccatccgc tatcttaaac
     8281 agcaaaatgt aagggtgatg ctggggcata gcgcggcgac ctggcaacaa acccgcgccg
     8341 cgtttgatgc cggtgccgac ggcctggtgc attgctataa cgggatgaca ggtttacatc
     8401 accgcgaacc gggaatggtt ggcgcgggat taacggacaa gcgcgcctgg ctggaactga
     8461 tagccgatgg tcatcatgtg catccggcgg caatgtcgct gtgttgttgc tgtgcgaaag
     8521 agagaatcgt gctgatcacc gacgcgatgc aggcagccgg gatgccggat ggtcgctata
     8581 cgttatgtgg tgaagaagtg cagatgcacg gtggcgttgt ccgtaccgcg tccggtgggc
     8641 tggcgggcag tacgctgtct gttgatgcgg cagtgcgcaa tatggtcgag ttgacgggcg
     8701 taacgcctgc ggaagccatt catatggcat cgctgcatcc ggcgcgaatg ctgggtgttg
     8761 atggtgttct gggatcgctt aaaccgggca aacgcgccag catcgttgcg ctggatagcg
     8821 ggctgcatgt gcaacaaatc tggattcagg gtcaattagc ttcgttttga tggtttgctc
     8881 ctttattggg ccttcacttc ccccgtaagg cctttctttt tctttcgttt tgatctgtgc
     8941 agcggtgtcg gatgcgacgc taacgcgtct tatgcgacct acggtacgcc gcatccggca
     9001 atcggtgaac atagcgacgt cattacgcaa ttctttaatc ttcccttgtc ttttctttca
     9061 atgatcacag atttcgtttt atttcctttt tctccattga actttcggtt tcttttctat
     9121 agattttaat caacgaaaga catcaccaag tgaaatgaaa cgaaaggcaa gtgaaagcga
     9181 caacgcccga cgtcaagttc atcagactaa ggattgagtt atgccagaaa attacacccc
     9241 tgctgccgcc gcatccggta catggactga agaagagatc cgccatcagc ctcgcgcatg
     9301 gatccgttca ctcaccaaca tcgacgcgct acgttccgcg ctcaataact tccttgaacc
     9361 gttactgcgc aaagagaatc tgcggatcat cctgaccgga gccggaacct cggcatttat
     9421 cggtgacatc atcgcgccgt ggctcgccag ccataccggt aaaaacttca gcgccgtacc
     9481 gaccaccgat ctggtcacta atccgatgga ctacctgaac ccagcccatc cgctgctgtt
     9541 gatctccttc ggtcgatccg gcaacagccc ggaaagcgtc gcagccgtgg aactggcaaa
     9601 tcaatttgta ccggaatgct atcacctgcc gatcacctgc aacgaagcgg gcgctcttta
     9661 ccaaaacgcg atcaacagcg ataacgcgtt tgccgtgctg atgcccgcag aaacgcacga
     9721 tcgcggcttt gcgatgacca gcagcattac caccatgatg gccagctgcc tcgcggtttt
     9781 cgcacctgag acgatcaaca gccaaacctt ccgcgacgtg gcggatcgtt gccaggcgat
     9841 cctgacctca ctgggcgatt tcagcgaagg tgtgtttggt tacgcaccgt ggaaacggat
     9901 cgtttatctc ggcagcggtg gcttacaggg cgcagcacgc gagtcggcgc tgaaagtgct
     9961 ggaactgacg gcgggtaaac tggcggcctt ttatgattct ccaaccggat tccgtcatgg
    10021 accaaaatcg ctggtcgatg acgaaacgct ggtggtggta tttgtctcca gccaccctta
    10081 cacccgtcag tatgatcttg atctgctggc tgaacttcgc cgtgacaacc aggcaatgcg
    10141 tgtaatcgcc atcgccgcgg aaagcagcga catcgtcgct gccggtccac atatcatcct
    10201 gccaccgtca cgtcacttta tcgacgttga gcaggcattt tgcttcctga tgtacgccca
    10261 gacgtttgca ctgatgcagt cgctgcacat gggcaatacg ccggataccc catcagccag
    10321 tggcaccgtt aaccgcgtgg tgcaaggcgt aatcattcat ccgtggcagg cataagagga
    10381 tcgcattatg agcattatct ccactaaata tctgttacag gacgcccagg ccaatggcta
    10441 cgcggtgcct gcctttaaca ttcataacgc cgagacgatc caggcgatcc tcgaagtgtg
    10501 cagtgaaatg cgatcgccgg tgatcctcgc cggaacgccg gggaccttta aacatatcgc
    10561 gctggaagag atctacgccc tgtgcagcgc ctattccaca acctacaaca tgccactggc
    10621 gctgcatctc gaccaccacg aatcgctgga tgatattcgc cgtaaagtcc acgcaggtgt
    10681 gcgcagtgca atgatcgacg gcagccactt cccgtttgcc gagaacgtga agctggtgaa
    10741 atcggttgtt gacttctgcc actcacagga ttgcagcgtg gaagcagaac tgggccgcct
    10801 gggcggtgtt gaagatgaca tgagcgttga cgccgaaagt gcattcctga ccgatccaca
    10861 agaagctaaa cgctttgtcg aactgactgg cgtcgacagc ctggcggtag cgattggtac
    10921 ggcgcacggc ttatacagca aaacgccgaa gattgatttc cagcggctgg cggaaattcg
    10981 tgaagtggtg gatgttcctc tggtgctaca tggtgccagc gatgttccgg atgaatttgt
    11041 ccgtcgcact attgaacttg gcgtcacaaa agtgaacgtt gccacagaat taaaaatagc
    11101 cttcgccggc gcggttaaag cctggtttgc ggaaaatccg cagggtaatg atcctcgtta
    11161 ttatatgcgc gtcggaatgg atgcgatgaa agaagttgtc agaaataaaa ttaatgtctg
    11221 tggttcagcg aatcgaattt cagcataatc attagttttt tatgatttat cccaatgtac
    11281 ttcccgatta atatcgggga gtgccttaat ggaaaaggag atgactaaac cttaataaat
    11341 acatcagtac aatatcgcaa caataatata tttaaaaaca ttatattatt caactttatg
    11401 gtgaggatta cacaatgagc agtccaaata ttctcttaac ccgtattgat aaccgtctgg
    11461 ttcacggtca ggttggcgtg acctggacat ccaccatcgg tgcaaatctg ctggtagtcg
    11521 tggatgatgt tgtcgctaac gatgatattc aacagaaatt aatgggtatt accgcagaaa
    11581 cctacggctt tggcattcgt ttctttacta tcgaaaaaac cattaacgtc atcggcaaag
    11641 ctgcaccgca tcagaagatc ttcctgattt gccgtacgcc acaaacggta cgtaaattgg
    11701 tagaaggtgg cattgacctg aaagatgtca acgtcggcaa tatgcatttc tcggaaggga
    11761 aaaagcaaat cagcagtaaa gtttatgtcg atgaccagga tctcacggac ttacgtttta
    11821 ttaaacaacg tggcgtgaat gttttcattc aggacgtccc tggcgatcaa aaagaacaaa
    11881 tccctgacta aatctaaaat cgccttaata ttggtttgag gtaataaaaa tgcatgaaat
    11941 aaccctactt cagggattat ccctggcggc gttagttttc gttctgggga ttgatttttg
    12001 gctggaagcc ttatttttat tccgcccgat aatcgtttgt accctaactg gcgctattct
    12061 cggtgatatt cagactggct taattaccgg tggtctgaca gagttggctt tcgccggatt
    12121 aacccctgca ggtggtgttc agccgcccaa ccctattatg gcgggtctga tgaccaccgt
    12181 cattgcatgg tctacgggcg ttgatgccaa aacggcaatt ggtcttggcc taccgtttag
    12241 tttgttaatg cagtacgtca ttctgttctt ctattctgct ttctcattat ttatgaccaa
    12301 agccgataaa tgcgcgaaag aagcggatac ggcagcgttt tcccgactta actggacaac
    12361 gatgctcatc gtcgcttcag cgtatgcggt gattgctttc ctctgtactt acctggcaca
    12421 aggggcgatg caggcgctgg tgaaagcgat gcccgcctgg ctgacccacg gctttgaagt
    12481 ggctggcggt attctgcctg ccgttggttt tggcttgctg ctgcgcgtga tgttcaaagc
    12541 acaatatatc ccttacctga tcgccggctt cctgtttgtt tgctacatcc aggtcagcaa
    12601 cctgttgccg gttgccgtac tgggcgcagg ctttgcggtg tatgagtttt tcaatgcgaa
    12661 atcccggcag caagcgcaac cgcagcccgt tgccagtaaa aatgaagaag aggactacag
    12721 caatgggatc tgaaatcagt aaaaaagata tcacccgtct gggctttcgt tcgtcgctgc
    12781 tgcaagcgag ctttaactac gaaaggatgc aggcgggcgg ttttacctgg gcgatgttgc
    12841 cgatcctgaa aaagatttat aaggatgaca aaccgggcct aagcgcagcg atgaaagata
    12901 acctcgaatt tattaatacc cacccgaatc tggtcggatt cctgatgggg ttattaattt
    12961 cgatggaaga aaaaggagaa aaccgcgaca ccattaaagg cctcaaagtg gcactgtttg
    13021 gcccaatcgc cgggattggc gatgcgattt tctggtttac tttgttgccg attatggcgg
    13081 gaatttgctc atcatttgcc agccagggaa acctgctggg gccgattcta tttttcgccg
    13141 tttacctgct tatctttttc ctgcgcgtcg gctggaccca cgtcggttat tcagtcggcg
    13201 tgaaggcgat cgataaagtg cgagagaact cgcagatgat tgctcgctcg gcaaccatcc
    13261 tcgggatcac ggtaatcggc gggctgatcg cttcgtatgt gcatattaac gtggtgacat
    13321 cgtttgccat cgacagtacc cacagcgtcg cgctgcaaca ggatttcttc gataaagtct
    13381 tcccgaacat tttaccgatg gcctacaccc tgctgatgta ttacttcctg cgggtgaaaa
    13441 aagcgcatcc ggtgctgtta atcggcgtga cttttgtgct ctctattgtt tgttccgcat
    13501 tcggcatttt gtaaatggaa cgaggcactg cgtctggcgg tgcctcttta cttaaggaat
    13561 ttcatcctgt gcaaaccctt cagcaagttg aaaactatac ggcgttaagt gaacgtgcca
    13621 gcgaatattt attggccgtg atccgtagca aaccggatgc cgtgatttgc ctggcgaccg
    13681 gagccacgcc attactgacg tatcattatc tggtagaaaa aatccaccag cagcacgttg
    13741 atgtcagcca gctcaccgtc gtcaaactcg atgaatgggt ggatctgcca ttaacgatgc
    13801 ctggtacctg cgaaactttc ctgcaacagc atatcgtgca gccgctgggg ctacgtgaag
    13861 accagctcat cagctttcgc tccgaagaga taaatgagac agagtgcgaa cgggtaacga
    13921 acctgattgc gcgcaaaggc ggtctggatt tatgcgttct cggattgggg aaaaacggtc
    13981 atcttgggct gaacgaaccg ggggaaagcc tacaaccggc ctgccatatc agtcaacttg
    14041 atgccagaac acagcaacat gagatgttaa aaaccgcggg tcgccccgtg actcgtggga
    14101 tcaccttagg cctgaaggat attctcaatg cccgcgaggt tttgttactg gtgactggcg
    14161 aaggaaagca ggatgcgaca gaacgttttc tcacggctaa agtctctacc tctatcccgg
    14221 cttcgttttt gtggctgcac agtaacttta tttgtttaat taatacctaa ccagatgaaa
    14281 aatttgctca aaatgtttaa attaacttat tcaacagtca cgcattatat taaataacat
    14341 ttgactgggt tgaacataac gccaatagca aaaggagtta tgttcaacaa tttaatttta
    14401 gatatacata taacttgttc tattaataaa ccatctcgta ttctgactcg ccccctggcg
    14461 cagcaaagtc atgcacttat actcttttga atacatagaa aa
//
LOCUS       DAZGCQ010000115        14388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000115 DAZGCQ010000000
VERSION     DAZGCQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.115"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..5779
                     /locus_tag="ACHK07_004069"
     CDS             <1..5779
                     /locus_tag="ACHK07_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8104341.1"
                     /translation="ADNGAAVASTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQSR
                     DVTFIADVRTAQIADLVVIKDSSEADGATANTLRARVTDAFGNALAGQTVSVLADNGA
                     TVSPTVTTEPDGTVEISVTSQTAGTSVVTVSINNSSLSRNVTFIADVRTAKIADLAVT
                     QDGSVADGATANTLRVRVTDAFGNTLAGQTVSVLADNSATVTPTVITGQDGTVEISVT
                     SQTAGTSVVTASINNSSLSRDVTFIADIRTAQIADLVVIKDGSEADGATANTLRARVT
                     DAFGNALAGQTVSVLADNGATVSPAVTTEPDGTVEISVTSQTAGISTVTATINSSSLS
                     RNVTFIADVRTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALGGQTVSVLADNG
                     ATVASTVTTGQDGTVEISVTSQTAGVSAVTATINNSSLSQSVMFIADVRTAQIAELVV
                     IKDGSEADGATANTLQVTVTDAFGNTLAGQTVSVLAGNGATVAPTVITEPDGTVEISV
                     TSQTAGISTVTASINSSSLSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLQVKV
                     TDAFGNALNGQTVSVSADNSAMVTPTVTTQPDGTVEISVTSQTAGISTVTATINNSIL
                     SRNVMFVADVRTAQIADLVVIKDDSVADGAMANILRARVTDAFGNTLAGQTVSVMADN
                     GATVALTMTTKPDGTVEISVTSQTAGISTVTATINNSTLSQNVTFIADIRTAQIADLV
                     VIKDDSVADGAMANMLRARVTDAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEIS
                     VTSQTAGTSAVTASINSSSQNRDVTFIADVRTAKIASLEVTQDNAVADGAMADMLRAR
                     VTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEISVTSQTAGVSAVTASINNSS
                     LSRDVTFIADVRTAQIADLVVIKDGVVADGAMANMLRVRVTDANGNTLGGQTVSVTAD
                     NSAMVASTVITGPDGTVELSVTSQTAGTSTVTASINNSSLSRNVTFIADIRTAQIADL
                     VVIKDDSVADGAMANMLRARVTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEI
                     SVTSQTAGISVVTASINSSSQSRDVTFIADVRTAQIADLVVIKDGSVADGAMANMLRA
                     RVTDAFGNALGGQTVSVTAGNSATVTPTVTTQSDGTVEFSVTSQTAGVSAVTATINNH
                     SLSQNVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAANS
                     VLTVDGTDPSETGSWVESGGVYTTTRMATIAGTDQHANLQLQTWSDGVTSDRYDIQSG
                     SPAQATSTIATDKNAYTAGETITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGE
                     WSETAGVYTATWSAQMAGDSHHATLTLPEWGNSKQSESYSIHSGAPVQANSAIRTDKS
                     AYIAGEPLTVTITLRDEFGNPALGLTSEVIESYIDSFAVGGATPDSMQWVEQNNGEYT
                     IVWTAWVAEENLVASLKLKTWATEIKSSLYGIQPGAAAQTQSTIVTDKTKYIAGDSIT
                     VTVVLKDAQGNFITDGVAQLNEENVQVRNADSIQGNNWVYNGNGQYQRQYMAHFAEAN
                     LNAQLKMAGWSDANYSKNYTINRGEVSMLRSQLRIREVLVVAGADIPVSVLLSDEFGN
                     PVNDGLDLLTDDAVYLQNVEKKHWSSWTFVGDGRYERTYMAYKEGENLNSYLHINGWY
                     VGGQPSYTILPFVEVESLSVNGAKFRAADGFPKTGFDGAKFTLILTHNMKNTDYNWTS
                     GIQGIQVDSNGMVTLEFIINKEVTITGTPKSNKGNKVTYKFSLQKWFIPQGIIQESWS
                     EMNSYCIGNGYILPSSTDLVGSSTSGAVPRKVGSLWGEYGNLTSYDGIFRAEHYWLDS
                     GMIFYPGDGHLSIAPRSSPLCMKTF"
     gene            complement(5829..7526)
                     /gene="eilA"
                     /locus_tag="ACHK07_004070"
     CDS             complement(5829..7526)
                     /gene="eilA"
                     /locus_tag="ACHK07_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO8104342.1"
                     /translation="MQLQHGKNNTQQYIFGDFVLKNNGILLFKNKEYHIPPKELGVII
                     LLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGY
                     RFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPA
                     SVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQSIDF
                     NPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESL
                     KRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGISEQKQAITTAVTSIEKALEINP
                     SNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLYFLNGDLARAFN
                     LIEKSIALEPNKMGISILKLIILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFM
                     ALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKIN
                     GVILPLIYSVYGKKEYEKRWQQLIKESDLWSNVLLHDPRLLSVKNEFNTIGVMRSSA"
     gene            complement(7729..9078)
                     /locus_tag="ACHK07_004071"
     CDS             complement(7729..9078)
                     /locus_tag="ACHK07_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFO8104343.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLVNSKSEGNL
                     HNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEEWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYLLIKNVLQLTENVNTIDGKLKDPAKILTLHNAVRKTLANVAHNTTG
                     PGAMATTKGTLTHVIASLDSFAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNGQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
     gene            complement(9122..10273)
                     /locus_tag="ACHK07_004072"
     CDS             complement(9122..10273)
                     /locus_tag="ACHK07_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104344.1"
                     /translation="MATYEIKNATSTLNINNLDKTIFDREHSKENNIPNDNKIVQYYN
                     LKDIKHDISSQFIILKNTDREVCPELRVKLLDNLNYSRNTLDNSKSDVDKATITSSQS
                     SCSTEQTTIKISKFNILDMLDNLTMRQQVVMEEFLRVFTSMYKASAMSEAIMSNVFEQ
                     LVQYQRKETERATAASSVGVIIGSGVGAAGAAASVVANYRSHPEKMVTLTPDQEHTIN
                     RTDDDVQRASKREVREQQASLKQIDEKIKNTTKDTSLNEEQKEEALKSYDKEKETTLE
                     KIRDTNEKISTKHNNRYNKYFTIGKADVLSQLSNESIRATSRFLESTTHAEKDIVEQA
                     KNVMHSVMSSYLDQINRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
     gene            complement(10286..12067)
                     /locus_tag="ACHK07_004073"
     CDS             complement(10286..12067)
                     /locus_tag="ACHK07_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HFO8104345.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAIQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     ATRLHNNLESTLLRHEMAHNKFRELSDAYSSSLDDAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVATIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIINSVSKNIIKVLDSVCSMIQTSVVVLKLIAK
                     ISNGLEKIGLLICETATSIMNYCVAENSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
     gene            complement(12123..12305)
                     /locus_tag="ACHK07_004074"
     CDS             complement(12123..12305)
                     /locus_tag="ACHK07_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104346.1"
                     /translation="MKINGKNASFTPEELARKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            complement(12315..12812)
                     /locus_tag="ACHK07_004075"
     CDS             complement(12315..12812)
                     /locus_tag="ACHK07_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFO8104347.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            complement(13114..13407)
                     /gene="yicS"
                     /locus_tag="ACHK07_004076"
     CDS             complement(13114..13407)
                     /gene="yicS"
                     /locus_tag="ACHK07_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO8104348.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAIERNNQNNYWEALGKVECPDM"
     gene            13616..>14388
                     /gene="nlpA"
                     /locus_tag="ACHK07_004077"
     CDS             13616..>14388
                     /gene="nlpA"
                     /locus_tag="ACHK07_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO8104349.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVA"
BASE COUNT         3807 a         3046 c         3482 g         4053 t
ORIGIN      
        1 ggcggacaac ggtgcagcgg tcgcttcgac gatgaccaca aagccggacg gcacggtgga
       61 gatctcagtc accagccaga ccgccggaac cagtacagtc actgccagca tcaacaacag
      121 cagtcagagc cgggatgtga catttatcgc ggatgtcagg acggcgcaga tcgcggatct
      181 ggtggtcatt aaggatagtt cagaggcgga cggtgcgacg gcgaatacgc tgcgggcgag
      241 ggtgaccgat gcgttcggca atgcgcttgc cgggcagacg gtcagcgtgt tggcggacaa
      301 cggcgcaacg gtgtctccga cggttaccac agagccggac gggacggtgg agatcagtgt
      361 caccagccag acggcgggca ccagtgtggt cactgtcagc atcaacaaca gcagcctgag
      421 ccggaacgtg acatttattg ccgatgtcag gacggcgaag atcgctgatc tggcggtgac
      481 gcaggacggc tcagtggcag atggcgcgac ggcgaacacg ctgcgagtga gggtgacgga
      541 tgcgttcggg aacacgcttg ccgggcagac ggtcagcgtg ttggcggata acagcgcaac
      601 ggtcactccg actgtcatca cagggcagga cggcacggtg gaaatcagtg tcaccagcca
      661 gacggcgggc accagtgtgg tcactgccag catcaacaac agcagcctga gccgggatgt
      721 gacgtttatc gccgatatca ggacggcgca gatcgcggat ctggtggtca ttaaggatgg
      781 ttcagaggcg gacggtgcga cggcgaacac gctgcgggcg agggtgaccg atgcgttcgg
      841 caatgcgctt gccgggcaga cggtcagcgt gttggcggac aacggcgcaa cggtgtctcc
      901 ggcggtcacc acagaaccag acggcacggt ggaaatcagt gtcaccagcc agacggcggg
      961 gatcagtact gtcactgcaa ccatcaacag cagcagcctg agccggaacg tgacgtttat
     1021 cgccgatgtc aggacggcgc agatcgctga tctggtggtt attaaggatg gctcagtggc
     1081 agacggttcg acggcgaaca cgctgcgggt gagggtgacg gatgcgttcg gtaatgcgct
     1141 gggcgggcag acggtgagcg tgttggcgga caacggcgca acggtcgctt cgacggtcac
     1201 cacagggcag gacggcacgg tggagatctc agtcaccagc cagacggcgg gagtcagtgc
     1261 agtcacggca accatcaaca acagcagcct gagccagagc gtgatgttta ttgccgatgt
     1321 caggacggcg cagatcgcgg agctggtggt tattaaggat ggttcagagg cagacggtgc
     1381 gacggcgaat acgctgcagg tgacggtcac cgatgcgttc ggtaatacgc ttgccgggca
     1441 gacggtcagc gtgttggcag gcaacggcgc aacggttgcc ccgacggtga ttacagagcc
     1501 ggacgggacg gtggaaatca gtgtcaccag ccagacggcg gggatcagta cagtcactgc
     1561 cagcatcaac agcagcagcc tgagccggaa cgtgacgttt attgcggatg tcaggacggc
     1621 gaagatcgct gatctggtag tcattaagga cgactcagtg gcagacggtg caatggcgaa
     1681 tatgctgcaa gtgaaggtca ccgatgcatt cgggaatgcg ctgaatgggc agacggtcag
     1741 cgtatcggca gacaacagcg caatggtcac tccgacggtg accacgcagc cggatggcac
     1801 ggtggagatc agtgtcacca gtcagacggc ggggatcagt acagtcactg caaccatcaa
     1861 caacagcatc ctgagccgga acgtgatgtt tgtcgcggat gtcaggacgg cgcagatcgc
     1921 tgatctggtt gtcattaagg acgactcagt ggcagacggt gcaatggcga atattctgcg
     1981 ggcgagggtg accgatgcgt tcggtaatac gcttgccggg cagacggtca gcgtgatggc
     2041 ggacaacggt gcaacggtcg ctttgacgat gaccacaaag ccggacggca cggtggagat
     2101 cagtgtcacc agccagacgg cggggatcag tacagtcact gcaaccatca acaacagcac
     2161 cctgagtcag aacgtgacat ttatcgccga tatcaggacg gcgcagatcg cggacctggt
     2221 ggtcattaag gacgactcag tggctgacgg cgcaatggcg aatatgctgc gggcgagggt
     2281 caccgatgcg ttcggtaatg cacttgccgg gcagacggtc agcgtgttcg caggcaacgg
     2341 tgccacgact gctccgacgg tcaccacgca gccggacggc acggtggaga tctctgtcac
     2401 cagccagacg gcaggcacca gtgcggtcac tgccagcatt aacagcagca gccagaaccg
     2461 ggatgtgacg tttatcgccg atgtcagaac ggcgaagatc gcaagtctgg aggtgacgca
     2521 ggataacgcc gtggctgacg gcgcaatggc ggatatgctg cgggcgaggg tcaccgatgc
     2581 gttcggtaat gcgcttgccg ggcagacggt cagcgtgatg gcggacaacg gtgcagcggt
     2641 cgcttcgacg atgaccacaa agccggacgg cacggtggag atctcagtca ccagccagac
     2701 ggcgggagtc agtgcggtca ccgccagcat caacaacagc agcctgagcc gggacgtgac
     2761 atttatcgcc gatgtcagga cggcgcagat cgctgatctg gtggtcatta aggacggcgt
     2821 agtggcagac ggcgcaatgg cgaacatgct gcgggtgagg gtcaccgatg ctaacggtaa
     2881 tacgcttggc ggacagacgg tcagcgtgac ggcagacaac agtgcaatgg tcgcttcgac
     2941 tgtcatcaca gggccggacg gcacggtgga gctctcagtc accagccaga ccgccggaac
     3001 cagtacagtc accgccagca tcaacaacag cagcctgagc cggaacgtga catttatcgc
     3061 cgatatcagg acggcacaga tcgcggacct ggtggtcatt aaggacgact cagtggcaga
     3121 cggtgcaatg gcgaatatgc tgcgggcgag ggtcaccgat gcgttcggta atgcgcttgc
     3181 cgggcagacg gtcagcgtga tggcggacaa cggtgcagcg gtcgcttcga cgatgaccac
     3241 aaagccggac ggcacggtgg agatcagtgt caccagccag acggcaggaa tcagtgtggt
     3301 caccgccagc attaacagca gcagccagag ccgggatgtg acgtttatcg ccgatgtcag
     3361 aacggcgcag atcgcggacc tggtggtcat taaggacggc tcagtggctg acggcgcaat
     3421 ggcgaatatg ctgcgggcga gggtcaccga tgcgttcggc aatgcgcttg gcggacagac
     3481 ggtcagcgtg acggcaggca acagcgcaac ggtcactccg acggtcacca cgcagtcgga
     3541 cggtacggtg gagttcagtg tcaccagtca gacggcggga gttagcgcag tcacagcaac
     3601 catcaacaat catagcctga gccagaacgt cacctttgta ccgggtgacg catcgcagct
     3661 cacttcaacc gttgaaacaa ataagtctaa ctacacggtt ggagagacca tcaccatcac
     3721 ggtaacgctc cgggatgcgt tcgataatct ggtaaccggt gcagcttcac agttagccgc
     3781 taatagtgtg ctgacggtgg atggcaccga cccgtcagaa acgggaagct gggttgaatc
     3841 aggtggcgtg tataccacaa cccgaatggc gacgattgcc ggtaccgatc agcacgctaa
     3901 cctgcaactg cagacgtgga gtgatggtgt aacgtcagat cgctatgaca tacagtccgg
     3961 ttctccggcg caagccacat caactatcgc cacggataaa aacgcataca ccgctggcga
     4021 gactataacc gtcgccgtga cgctgaaaga tgcgcacggt aacctggttg aagggggcga
     4081 gtccttactg tctggtgata acgtaacagt ggaaggagcc gtacgttcag gagagtggtc
     4141 tgaaactgcc ggtgtttata ctgctacatg gtcggcgcaa atggcgggag actctcacca
     4201 cgcgacgctg acgttgcccg agtggggcaa cagtaaacaa tcagagagtt attccatcca
     4261 cagtggtgct ccggtgcagg caaattctgc tatcaggaca gataaatcgg catatatcgc
     4321 cggggagcca ttaactgtca caataacgtt gcgggatgag tttggtaacc ctgctttagg
     4381 gctgacatca gaagtcatcg agtcttatat cgatagtttc gcggtaggtg gcgcaactcc
     4441 cgattctatg cagtgggtcg agcagaataa tggtgaatat accattgtct ggacagcatg
     4501 ggttgccgag gaaaatctgg ttgccagtct gaaattaaaa acatgggcaa cggagattaa
     4561 gtcctcatta tacggaatac aaccaggtgc ggcggcacaa actcaatcca cgattgtcac
     4621 ggacaaaacg aaatatatcg ctggggatag cataacggtg accgttgttc ttaaagatgc
     4681 ccagggtaac tttattactg atggtgtcgc gcagcttaat gaggaaaacg tacaagtcag
     4741 gaacgctgat tctattcaag gcaataattg ggtttacaac ggcaatgggc aatatcaaag
     4801 acagtatatg gcgcattttg cggaagcgaa cttgaatgcg caactgaaga tggctggttg
     4861 gagcgatgcc aattattcaa aaaactacac tattaacaga ggtgaagtaa gtatgcttcg
     4921 ttcacagctt cgtatacgcg aagttttagt ggtagctggg gcggatatac ctgttagtgt
     4981 tttattatct gatgaatttg gtaatcctgt gaatgacggt ctggatttgc tgacagatga
     5041 tgcagtgtac ttacaaaatg tggaaaagaa acattggtcc agttggacat ttgtaggtga
     5101 tggcagatat gagcgtacct atatggctta caaagaagga gaaaacttaa actcttatct
     5161 acatattaat ggttggtatg taggcgggca accgtcttat accatccttc cttttgtgga
     5221 agtcgagtcg cttagtgtca atggtgcaaa attcagggcg gcagatggtt ttcccaaaac
     5281 agggtttgat ggtgctaaat ttacactgat attaacccac aatatgaaaa acacagatta
     5341 taattggacc agtggcatac agggtattca ggtcgatagt aatggtatgg tgactttaga
     5401 attcattatc aataaagagg ttactattac aggaacgccg aaaagcaata aagggaataa
     5461 agttacatat aaattctcac tgcagaaatg gtttatacct caagggataa ttcaggaatc
     5521 atggagtgag atgaattcat attgtattgg taatggttat atactaccgt ctagtacgga
     5581 tttagttggg agttcaacta gcggtgctgt cccgcgaaaa gtaggttcgt tatggggtga
     5641 atatggtaat ctgacttcgt atgacggaat atttcgcgct gaacattatt ggctcgacag
     5701 cggtatgata ttttatccag gagatggtca tctctcgatt gcgccacgtt catccccact
     5761 gtgtatgaaa acgttctgat attatttatg ttattgccct gcatcagcag ggcaatttat
     5821 cttcgctctt acgcagaact ccgcatcaca ccaatagtat taaattcatt tttcacactg
     5881 agcaaacggg ggtcatgaag caacacattt gaccataaat cactctcttt aattaattgt
     5941 tgccagcgct tttcatattc ttttttacca taaacactgt aaattaacgg caaaataaca
     6001 ccattgattt tgttggtgtt aatatttgcc agtaacttca taatgtcatt ttttatagat
     6061 gcgccataga tcagaaactt cgtataaagc gagttaacgc aggtgtaatc aagaccatgt
     6121 tcaggttcaa gtttaagcaa taaacttttg gctttgtcat tatgtccttt cagtgccatg
     6181 aacatagcca gaatagatgt aataattgga ttgttacagg tgttttgact gttaagattt
     6241 aatgcaaaag atatcgcgtt atcgagtggt gatgtataat aaagtataat taatttaaga
     6301 atgcttatgc ccattttatt gggttcaaga gcaatgcttt tttctattaa attaaatgct
     6361 cttgcaaggt cgccgttcaa aaaatatagc agcgactgat aataataaac gtctggcgaa
     6421 ttaggtttga gtaaatgtgc ttgtttaaat agcacattag acactgaatg ttcatctttt
     6481 agcccactga ttaaacccag taaacccaat gcctgagaat ttgaagggtt aatttccaga
     6541 gccttttcaa tggaggttac tgccgtagtt attgcttgtt tttgttcact gattccaagg
     6601 agtgctaatg atatatggca ttccgccagg caacagtagg gcaaggtatt ttgcggttgc
     6661 gtactcacac aatctctaaa tattactaat gctcgtttca aactttctgg tgtataacgg
     6721 tacatttcca ttctgccatt catatacata acagcagaat ccagagatgg catttgttgt
     6781 gtattaatgg atttaaaatt aatgttgggt attttttcaa taagaatatt aattacttgt
     6841 gataatagca cggaaagagg attgtcgggg ttgaaatcaa tactttgatg atcaatcaat
     6901 ttatgcgttc tggcatggat taattcaata aataaacgcc agtttttacc atagtgaacg
     6961 acctgaccca taatataata ttctgggcct gttttattga taaactgatg gatagtggag
     7021 aaatcactgg cttcattcgt taccgaagcg ggtaaaatat ctaaaccaca aaaagcatat
     7081 tttgaaaggc cctggaccag ttcgtaatgg agttttagaa cattgatcga accttcagta
     7141 cgaaagggaa aaacggcaag agtggtggtc gactttaccg gttcattatc ggttacaatc
     7201 acaatgggga cagtaaaacg gtatccacga ccatagaccg tttcgatata cttacactgt
     7261 ttattctcat gcaaaagttt acgcaatgca taaatacacc gtgttaaaga ttcatcactg
     7321 gcaacactcg cgctccagac cttatcgata atctcttctt tactaacaat ttcaccgtca
     7381 gcattaagta atagaatgat aacgccaagt tctttcgggg gaatatgata ttctttgttt
     7441 ttgaataata atattccatt gttttttaat acaaagtcac caaatatata ttgttgtgta
     7501 ttattttttc catgttgcag ttgcatagaa cgctccttcc attttctcta ttcatagtat
     7561 taatttgatc tgtctaaaat aaagtattgg cagaattgct ctatcaaaaa aaactcattt
     7621 tgtgagattg tgatgaagaa atgaatgaaa gctgatgctt aataatatga aacactggtc
     7681 ccattttacg ggaccagata gtggacgata attgtaggtg ttacttaatc agaaacgtaa
     7741 ataatctttc gctgaatcaa acaacgcagt aatggttgaa gataataatt ttataattgt
     7801 atcgtaggtg gagttggctg tactgtattt agtcgttata acctgcacgt cggttttcat
     7861 gttttctgcc tggccattat aacccgccaa ccatgcctgg tattcagccg tgcttatttc
     7921 gtagccggtg aattctttag atatattttt ttccttccct ttatcatccc tgtgaaccat
     7981 tgagtctgaa gactggctca tactgtcaat aatctgaggg ttgggaataa taacatacag
     8041 accgctacct tgcggttcaa cttcgactat aggagaaggt gtgcggtgtt ttgggtcggg
     8101 tggaataacc ggaagtccta aagcggcgca ccatttttct gcttcttcca gtggaacacc
     8161 aattttgtca gcaggtgccg gagggtataa aacatagtct ggattgtaat gataatgaaa
     8221 atcaccatta ggatatttat cgatataata aaaatgcatc aaggcttttt ggaatgcatt
     8281 gacatcaaaa actatcgcat cgcctttttt attagagtga gtccattttc ccatattgct
     8341 ttggatatgt tcactgaagt cctgccagta tgcgctcatt atctcaacgc tatttgcata
     8401 gatttcgagg tagtcactac cgagcacact catcaaatca gcaatatcac tacatatata
     8461 tttatcactt ttaaattcgc ttgaactact ggccgttaaa gtactgctgg ttgctgaact
     8521 tctcatttca ggcgcaaaac tatctaaaga agcgataacg tgagttaagg ttcctttggt
     8581 cgttgccatt gcacctggcc cggttgtgtt gtgagcaacg ttagccaggg tttttctcac
     8641 agcgttatgc agggtcaata ttttagcggg atcctttaat tttccgtcaa tagtattaac
     8701 attttctgta agctgcagaa cgttttttat cagcaaataa ctttcgtgta agagcgcaga
     8761 tcctttctct gacaggttct cagttgggaa atttctgagc atgtttttaa cgaagttaag
     8821 ttgtatgtcg tgctggcatt tccattcttc atttcgcggg ctacgttcac tgaccaaagt
     8881 tgatagtttc tcaacatctt tcttgagcat agcgccaata ttattatcct tactggcgct
     8941 gttatgcaaa tttccctcgc ttttgctgtt taccagttga tgaggctcct cactttgcga
     9001 ggatgcctct ttgagaggaa tgtttgcatt ctgctgacgt agaatatcta tatttaaatt
     9061 attatcttta ttaatcatga gtcattcctt cttaaatata attaactatt attgcaatta
     9121 tttatgaacg aatgccttga acgatagcac tatcagtatc aataatggtt tgtattattt
     9181 gctgtaataa attgagtgcc tgatctcttc cttgaacatc acgattaatt tgatccagat
     9241 agctactcat aactgaatgc atgacatttt tagcctgttc aacaatgtct ttttctgcat
     9301 gagtagtgct ttctagaaat ctagatgttg ctcgaattga ttcattgcta agctgtgaaa
     9361 gtacatctgc tttacctata gtaaaatatt tattatacct attgttatgt tttgtactaa
     9421 ttttctcgtt tgtatctcta atcttttcta gagtagtttc tttttcttta tcataagatt
     9481 tcagcgcctc ttctttttgt tcttcattaa gactcgtatc tttcgttgta ttttttattt
     9541 tttcatctat ttgtttgaga gaagcttgtt gctctcgaac ttcccttttt gacgcgcgct
     9601 gtacatcatc atcagttcta ttgatagtat gctcttggtc aggtgtaagg gttaccatct
     9661 tttcgggatg gcttctgtaa tttgctacga cagatgccgc ggccccggct gcgccaactc
     9721 ctgacccgat aataacacct accgaacttg ctgctgtggc gcgttcggtt tcttttctct
     9781 gatactggac aagttgttca aaaacattac tcataatagc ctcagacata gcagaggctt
     9841 tatacattga tgtgaatacc cttaaaaatt cctccattac aacctgttgt cgcatggtca
     9901 aattatctag catatcaagg atattaaatt tagatatttt aatggtggtc tgttctgtag
     9961 aacagcttga ttgactggat gtaattgtag ctttgtcaac gtcgctcttc gaattatcta
    10021 gtgtatttct tgagtaatta agattatcca atagttttac tcttagttcc ggacagactt
    10081 ctctgtctgt attttttaat attataaact gagaacttat gtcatgctta atatctttaa
    10141 ggttatagta ttgcacaatt ttattatcat ttggtatgtt gttttctttg ctatgctctc
    10201 tatcaaagat tgttttatcg agattgttaa tattaagtgt gctggtcgca tttttaattt
    10261 cataggtggc catgttagaa ccctcttata cgatagaatg ggaagcaaat tttaaggctt
    10321 cagttcggtg ttgtaatact cttaccattt ggcttacctc ttgttcgacg gttttatcca
    10381 ccctttgcaa tactgaaagc atttgttcac gcgtttttga tagattactc atgtcctgtt
    10441 gtaaaattgc catgtcggca gagttttcag caacacagta attcattatc gatgttgctg
    10501 tttcacagat aagtaagcct attttctcca ggccattgct tatcttggca atcaacttca
    10561 aaactacgac tgatgtttgt atcatactgc atacgctgtc taataccttt ataatatttt
    10621 ttgaaacgga attaataata gattcaatgg ttgattttat agtgcttgtt atttctttgg
    10681 tgacttcttc tgcggctgtt tttgataatg cagaaacaac ggcccctatt gctacagaac
    10741 atattacagc tgcaccaatc attactgtcg ccatgacaat agaagcggct gcacttgcgt
    10801 aaatactttt aaccagttca atcgtatctt gatcgacacc aataacgtcc agaagcgcgg
    10861 tgattgcgag cagagtggga tcaactgttt taatagcagc cgaaagacct tctacaactt
    10921 tccctaaaat ccagccggta gccgtggtgc ctagagcgat ttgacaagtg atatcaacac
    10981 ctaataccac tgcgcccgct attccaccaa taagcgccag tgcacccgca atacttgaac
    11041 caccactagg aactgcagca agaagaccaa caacaattaa gattgcactc atggcaatgg
    11101 tagctacagt tacacactca ttaacgattt ttaaaacttt agtcgtggta gtatatttac
    11161 gcgctgtttc attgagttta ttttccctga catggttaat ctctttcatt acctcagatt
    11221 gatttttaat agagtcgcaa ttggtatcac caataacttc cattactgag gtaatcaaaa
    11281 ggataaatgt ggctgtcgcg tttctatttt tctggttttc ttgggtcagg caaatattgg
    11341 tggtgaagtt attaatctgt tcttgctgtt gaatttcatc aaaaatttcc atactttggc
    11401 cgatcgcagc atcagcatcc ataatggctg tatttaacga tttctcagct aacgattttt
    11461 tttgtaatga cagcgtgagt gttttttcag ccgcattttt ttgcgtcagt actttattat
    11521 attgcggatc gccaggttgc agttgtgaca actcttgatt caatgtgttg acttttttct
    11581 ccagcgattg caccttttta tcgaccgcat tgtaattatt attggcctga tgcatgatat
    11641 cgtcggcttt ttgcgcatca tctaatgatg aggagtaagc gtcacttaat tcacgaaact
    11701 tattatgtgc catttcgtgt cgaagcaggg tgctttcaag gttattatgc aatcgtgttg
    11761 caatatcctg atcggcaatg cctgttaaat tacccaaaat cattgtcagg cgggcatcat
    11821 tcgtctttgc tgtagtggcg ttgtctgaat ctgaaacctg tattgcaggc ggagcaagca
    11881 tcggtttgtt ttcaagacga gcgcgaatat cgttatcaag ggatttcggg tttttattaa
    11941 gccgcatggg atcaacttgc gttttgctgc tgtcctgcag tcttttttcc gcatcgctat
    12001 actgtgtacg caatgtttcg ctgatttgtt caaacgtgat cgtttgtccc gtaatcggtg
    12061 ccgacataat tcacctcctt tttaagctat caccgggtgt aattaattct tttttattta
    12121 actcatggca aatgtttatt cccatctaaa atggcatcaa cgggaaagta agtaatgtgt
    12181 ttattgttat tcttctcatt tattttatcg ccatggttat tttttaaatc aaccggggct
    12241 tcctcatgat atttcctggc tagctcttca ggcgtaaaag aagcgttttt tccgtttatc
    12301 ttcattttgc tgactcactc tgtttgaact tcctcttcgg tgttggctgt caaaagttca
    12361 agataagcag tagccatctc tttaactttt tcattttgac tgtaggttac gactgtatta
    12421 aataactccg tggccatttt aatgtttttt aagcccatct ggcattgacc catataataa
    12481 accaaagaga aatcgttatc gggagatagc attaaacaga tatgatacat gtcaaatgcc
    12541 ttttgataat ctttcttcaa ctgacaaacg gcggcatatc cttttaagta gttataattt
    12601 tggaaatcat aaatacagag aaatttaaaa aagagttcag cttcgtcaag tcttcctttt
    12661 tcataaaagt tataagcgta agcataaacg ccttcaaggg tatccggagt aatgccatgc
    12721 agctctgcaa gtgttgctcc catacctagt gcacgttcga acttactggc aatttcttta
    12781 ttttcttcta aatccagatt tgcgctttcc atctctactc tctcctttcg aacattcttt
    12841 gctcagactt caccattttt tagggggtgg tgatatcaaa attcgctcat ttttatgccc
    12901 atccgttggg cttcgttttc tgcattaaaa acaagtattg tttcaatgag ataagatttt
    12961 ctatgtgatg aaatgaaaat aatgtagagg gaaatttatc cacaatcctg atgaactggt
    13021 tcaattttga ttggaatttg aaaaagcagg tgatttactg atgtgcgagc ttaagtcagc
    13081 ggctgctggc ctgagcgcgg aggtgtgcga aattcacata tccgggcatt ctaccttacc
    13141 cagcgcttcc cagtagttat tctgattatt acgctctatt gccacaatag cttcgcggat
    13201 atgttgtttg ttactctcat cagacaacat taatttttcc cacgcttcgt ctgataatga
    13261 cgcctgtggc gtgcaaattt tacgtaaatc ttgcaatacc gtggttttct cttttgccga
    13321 ctgtagcgaa ctgaatggcg actcggcctg agaaatggct ggaaaagtca ggaatacggt
    13381 aatcatgagt agcatcgtta gcttcatagc ggcaaccttc tgcaaatgtg catcgaatga
    13441 agcataagtg tagtttgctt ttgccgctct catcatgctg tacgaaaggt gtttgcgtgg
    13501 tcagtaagaa gtgccagttt tttatacctt tttcttctat tcgatagctt ttcgtttttt
    13561 aaatcgtagt gatcttgcca ttatagtcag ttaatgatta aaaaaaggat aaaaaatgaa
    13621 actgacaaca catcatctac gggcgggggc cgcattattg ctggcaggaa ttctgctggc
    13681 aggttgcgac cagagcagca gcgatgcaaa acacattaaa gttggcgtta taaatggcgc
    13741 agaacaagat gtcgcggaag tcgctaaaaa ggtggcaaaa gagaaatatg gcctcgatgt
    13801 tgaactggta ggttttagcg gttcgttgct accgaacgat gcgacaaatc atggcgaact
    13861 tgatgcgaac gtcttccagc atcgaccctt tcttgaacaa gataatcagg cgcatggtta
    13921 taaactggta gcggtgggaa atacgtttgt cttccctatg gcgggttatt ccaaaaaaat
    13981 caaaaccgtg gcgcaaataa aagaaggcgc gacagtggcg atccccaacg acccgaccaa
    14041 ccttggtcgc gcacttttac tgttgcaaaa agagaagcta attactctga aagagggaaa
    14101 agggttatta cctaccgcgc tggatataac cgataatcct cgtcatttgc agattatgga
    14161 actcgagggg gcgcaactcc cgcgcgtact ggatgatccc aaagttgatg tagcgattat
    14221 cagcaccact tacattcagc agaccgggct ttctccggtg cacgacagcg tatttattga
    14281 agataaaaat tcgccgtatg tgaatatttt ggtggcacgg gaagataata agaatgcaga
    14341 aaacgtgaag gaatttctgc aatcttatca atcatccgaa gtcgctaa
//
LOCUS       DAZGCQ010000116        14347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000116 DAZGCQ010000000
VERSION     DAZGCQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.116"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(95..847)
                     /gene="tcyN"
                     /locus_tag="ACHK07_004078"
     CDS             complement(95..847)
                     /gene="tcyN"
                     /locus_tag="ACHK07_004078"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO8104350.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGESKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(844..1512)
                     /gene="tcyL"
                     /locus_tag="ACHK07_004079"
     CDS             complement(844..1512)
                     /gene="tcyL"
                     /locus_tag="ACHK07_004079"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO8104351.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1527..2513)
                     /gene="dcyD"
                     /locus_tag="ACHK07_004080"
     CDS             complement(1527..2513)
                     /gene="dcyD"
                     /locus_tag="ACHK07_004080"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO8104352.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNVLGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2618..3418)
                     /gene="tcyJ"
                     /locus_tag="ACHK07_004081"
     CDS             complement(2618..3418)
                     /gene="tcyJ"
                     /locus_tag="ACHK07_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8104353.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3506..4057)
                     /gene="fliZ"
                     /locus_tag="ACHK07_004082"
     CDS             complement(3506..4057)
                     /gene="fliZ"
                     /locus_tag="ACHK07_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO8104354.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPSSDIY"
     gene            complement(4103..4822)
                     /gene="fliA"
                     /locus_tag="ACHK07_004083"
     CDS             complement(4103..4822)
                     /gene="fliA"
                     /locus_tag="ACHK07_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO8104355.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5142..6854)
                     /locus_tag="ACHK07_004084"
     CDS             complement(5142..6854)
                     /locus_tag="ACHK07_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO8104356.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVDAKITFGSGMTVDFTQASKNVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEATTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            7120..8526
                     /gene="fliD"
                     /locus_tag="ACHK07_004085"
     CDS             7120..8526
                     /gene="fliD"
                     /locus_tag="ACHK07_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO8104357.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8551..8961
                     /gene="fliS"
                     /locus_tag="ACHK07_004086"
     CDS             8551..8961
                     /gene="fliS"
                     /locus_tag="ACHK07_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO8104358.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8961..9326
                     /gene="fliT"
                     /locus_tag="ACHK07_004087"
     CDS             8961..9326
                     /gene="fliT"
                     /locus_tag="ACHK07_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO8104359.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9404..10891
                     /gene="amyA"
                     /locus_tag="ACHK07_004088"
     CDS             9404..10891
                     /gene="amyA"
                     /locus_tag="ACHK07_004088"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8104360.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10925..11338)
                     /gene="yedD"
                     /locus_tag="ACHK07_004089"
     CDS             complement(10925..11338)
                     /gene="yedD"
                     /locus_tag="ACHK07_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO8104361.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKAGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11525..12730
                     /gene="yedE"
                     /locus_tag="ACHK07_004090"
     CDS             11525..12730
                     /gene="yedE"
                     /locus_tag="ACHK07_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO8104362.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12727..12960
                     /gene="yedF"
                     /locus_tag="ACHK07_004091"
     CDS             12727..12960
                     /gene="yedF"
                     /locus_tag="ACHK07_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO8104363.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13069..13737
                     /locus_tag="ACHK07_004092"
     CDS             13069..13737
                     /locus_tag="ACHK07_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO8104364.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            13848..14327
                     /locus_tag="ACHK07_004093"
     CDS             13848..14327
                     /locus_tag="ACHK07_004093"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8104365.1"
                     /translation="MFTIKTDDLTHPAVQTLVAYHISDMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGMGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAQD
                     RCLHRLSLETGTQAGFTACHHIYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELL"
BASE COUNT         3477 a         3708 c         3605 g         3557 t
ORIGIN      
        1 attaatgagt tatgctgatt tgttaaacag ttttatcacg cttgaaatgg cgttccgccc
       61 cgactgtcga atcgtcgggg ctgatttttt cttattattg cagcagaaac ttctcgagga
      121 actggcgggt gcgaggctgc tcgggatcgg caaataacgc ttttgcggcc ccctgctcga
      181 ctatccgccc ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt
      241 cgtgcgtcac aatcaccatc gtgcgctttt cctgcgccag ctgacggatg gtgttcagga
      301 cttcgcccac cagctctgga tccagcgctg acgttggctc gtcaaacaaa atcacctcag
      361 gacgcattgc cagcgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac
      421 gtggatagct ggtttcttta ccggccagcc caactttcgc cagtagctcg cgagcgcgcg
      481 ccgtggcctc ttctttcgat tcgcctttga cgatcaccgg cccttcaata atgttctcca
      541 gcaccgtacg atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca
      601 actggcgaat cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc
      661 caacggtgat agttcccgct tccggttgtt ccagcagatt tatgctgcgt agcaacgtgg
      721 ttttgccgga accactcgga ccgataattg ccaccacttc gccaggcttt acctcaaggt
      781 cgataccgtg cagcaccgtc tgaccgtgaa attttttcac caggttctta acttcaatgg
      841 cactcatttt ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt
      901 cgataacaca gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac
      961 ctccagcgtg cgcgaggtaa tcaactgcgc ttgacggaac agctccggca cctggattgt
     1021 cgcggccagc gacgtatctt ttaccaggct gataaagctg ttcgacagcg gcggcaacgc
     1081 cacccgcgcc gcctgtggca aaatcgcgcg acgcatagtc tgccatggcg tcataccaat
     1141 actggcagct gcttcccact gacctttatc aatagaagaa atggcggcgc gaagcgtttc
     1201 ggcagcatag gcggcagtgt tgagcgacag accaatcatt gctgacggaa tgggatctaa
     1261 ttcgatacca aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt
     1321 accacgaaaa atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag
     1381 gcgcatcagc gcgagaataa acccgagcag taagccaaaa aacatgccgc cgatactgag
     1441 ttgcagcgta tacccagccc ctttcagcag gaacggcaag gaatcaataa ccagttgtat
     1501 actttcttgc atgagcgttt ccggagctaa acgtggggat gataggcgaa cagcgcaggc
     1561 gcgccgccgg tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata
     1621 ccgtcaatca gacccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc
     1681 agccgcgcca gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt
     1741 gcaaaatagt catcccagag taaaatttct gctgatgcgg tcagctccag ttctttcgca
     1801 atcgcctgtt gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc
     1861 acgccaatca gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga
     1921 gttccggcac tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt
     1981 tgcgcgattt ccagcgcact ctccacataa cctagcgcgc caagaacatt agaaccgcca
     2041 accggaatga catatgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt
     2101 tgggcattgg gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc
     2161 aacaagcgat tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc
     2221 agcgccacgc agtgcagacc gagtttggca gcgactgcgg cagtctggcg cacatggtta
     2281 gactggatcg ccccggcagt aatcagcgta tcggcacctt cacgcagagc atctgccgcg
     2341 agaaattcta gcttacgtaa tttattgccg cccattgcca tgggggtgac gtcatcccgt
     2401 ttgatgaaaa tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt
     2461 ggcgcgccga taaactccag ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc
     2521 tccgatgtgt gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag
     2581 gcgctagtga aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa
     2641 accatttttc ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat
     2701 tcactgcttt cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg
     2761 agaatgcttc accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg
     2821 ccagacgatc aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg
     2881 ggtcatcatc ataggtacgc acatcgacgc cctgaacatt ctgccgcagc cactcttcat
     2941 agttggtgcc cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg
     3001 tgccttcgtt accttttttc accagcgcct gaataccaga aatggtgtac ggggttgaga
     3061 aatcgtattt tttcttgcgc tcatcagaaa tggtgacctg attaatcacc acatcaatac
     3121 gtttagagtc cagcgacgcc agcataccgt cccatttggt cggttttagg gacgcctcaa
     3181 cgccaagatg ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt
     3241 catccccctg aaaactgaac ggcggataag ttccttccag acctaccagc agcgtgccgc
     3301 gctctttaac tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa
     3361 ccagcgccac ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat
     3421 tcaccccgaa tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa
     3481 caactccgct acatcttatt cttatttaat atatatcaga agaaggcagg ctggaggatt
     3541 tctgcacaag tccggtacag gtttggcgct gatagtgttg atacttccgt aacgcgatgc
     3601 ggtaattgtt ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt
     3661 cctgcagctg atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcagac
     3721 gaacgacata ttcacgcacc gtaccagggc tcatttcagt ttgctcaaaa agaaattgct
     3781 taaagccgat aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc
     3841 ggcacaatgc cgcccatgat ttttgttccg tttgccagcc attttcatca agcagcgtgt
     3901 ccaggcggga aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat
     3961 cgagtaattt acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc
     4021 ggcttaatgg ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt
     4081 taaagtgcgg catttactga cgttataact tacccagttt agtgcgtaac cgtttaatag
     4141 cctggctgtg taactgactg acccgcgatt ccccgacctc cagcactgca ccaatctctt
     4201 tgagattcag ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg
     4261 tttcgatggc ttccatcacc cgctggcgca gattattgtc cagcagttgt tgtagcgggt
     4321 tttctcgctg atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact
     4381 catcgtagga gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa
     4441 tatcgatccc taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct
     4501 ccagttgccc tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc
     4561 agtcacggct gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg
     4621 taaatgccgt tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc
     4681 ccgcctgtag cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg
     4741 cttcgtgacg caccagcggg acataacgct gccatagcga gtgtttatcc attacacctt
     4801 cagcggtata gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat
     4861 tatccgtttc tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg
     4921 gggttaccgg taaattgcgg acagaaaaac ccccgccggt ggcgggggag cacgttgctg
     4981 acaaattgcg cttgatgttg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaaatg
     5041 tgcaaattca ataaattgca attcaccgtg taggcctgat aagcgcagcg catcaggcaa
     5101 tttggcgttg ccgtcagtct cagttaatca ggttaggacg attaaccctg cagcagagac
     5161 agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc ctgctggatg
     5221 atctgcgctt tcgacatatt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac
     5281 tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccagacgg
     5341 ttctgcaccg caccgaggga agaacggaat ttgtctacag atgcgatagc atcgtccagc
     5401 gctttcagcg gatccgtggt cgcttcggat ttaccagtag cttcagtggt cagagaaccg
     5461 tcagccgctt tgtacagagc cgcgccgtca tcatccagaa tattgccgtt agcaaattcg
     5521 tgataagttt ctgtgacagt cttgttggtc gcagtcgtca gttcaccatc agcaccaata
     5581 tttacggtag cgccacctgt agtagcggtt actgccccac cagcggcaac gtcaaaagac
     5641 tgtgcgccgt tagctacggt ataagcctgc ccagttaacg cagtgtccat atcttcggcg
     5701 gataccgttg cgcccttaat atccacattt ttgctagcct gggtgaaatc aacggtcatg
     5761 ccgctgccga atgtaatctt cgcatcaact gctgcattac cattcgcacc gttgaaaata
     5821 ccgtccaacg ttgccgcatc tgggccgccg gcctggtttt tagtcaggtt gccgtcagaa
     5881 ccaatatata aagcatcacc attgacgtca gtcagggtgc catcgctggc aattttaacg
     5941 tcctgagcgc ttccgccgat ggagatctga gccgtattat caccgtttgc tgcataggct
     6001 ttcagcacat cagcaacttt agtggtggcc ccagtaccat cgccattggt aagtgcggtt
     6061 gcgtcaaatg aataagactt gctggttgcg ttgtaggtat atgcagtaga agccgctacg
     6121 cctaatccat tgtttgtacc ggtataaccg ctaatagtgc tgcctccgct aacgcttgcc
     6181 agcacgtcgg tagctgttgc tgcattaaag gtagtaacag tagatttagt ccaggtgctg
     6241 ttgccactga cgactgcacc ttgggagaag cccgcagcgg tcagatctgc ttcagtggct
     6301 ttagcattat caacagcgcc tttaccgtta acattgaagc tggtcagctt caatgtagaa
     6361 gagtcgatct ttttcagatc aatcgtgatg gtttcaccat catttgcgcc gacctgaatt
     6421 ttcatcgagc catctttaga cagcacgttc acgccgttga actgggtctg accggatacg
     6481 cggtcaattt cgtccagacg agatttgatt tcgtcctgga tggagtccag gtcagaatcg
     6541 gagttagtac cggtagtggc ctgaaccgtc agttcacgga tacgctgtaa gttgttgttg
     6601 atttcggaca gcgcgccttc ggtggtctgt gcaacagaaa taccgtcgtt ggcgttacgt
     6661 gcagcctgag tcaggccttt aatattagag gtgaaacggt tagcaatcgc ctgacctgcg
     6721 gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac
     6781 agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta
     6841 atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt attgggtttc
     6901 cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa
     6961 tttttttcac aaacagccat tttttgttag tcgccgaaat actcttttct ctgcccctta
     7021 ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc
     7081 ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg
     7141 ggagtcgggt caggtctgga tttaagttca atcctcgata gcctcaccgc cgcgcaaaaa
     7201 gcgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac
     7261 ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc
     7321 gatctttttt ccgccaccag cactaccagc agcaccaccg cgttcagtgc caccaccgcg
     7381 ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc
     7441 ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag cgacagtaaa
     7501 ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag cgcggctaac
     7561 tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc
     7621 atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt
     7681 gacaatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat
     7741 gacgccagtg ccagcagcaa cggcatggaa gtctcggtcg ccgcccagaa tgcgcagctg
     7801 acggtcaaca acgtcgccat tgagaacagc agcaacacca tcagcgacgc gctggaaaac
     7861 atcaccctga acctgaacga tgtcaccacg ggcaaccaaa cgctgaccat cactcaggac
     7921 acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc gctaatagat
     7981 accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct
     8041 agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca gttgaaatcg
     8101 atgctgagta ataccgtcag ttcttccaac tataaaacgc tggcgcagat tggtatcacg
     8161 accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa
     8221 aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcacg
     8281 accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct
     8341 accgatggcg ttagtaagac cctgaataaa ttaactaaag actacaacgc cgccagcgat
     8401 cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga cgttttaatg
     8461 acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa cagtaattcc
     8521 aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat
     8581 gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct ggtcaccatg
     8641 ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca ggacaacaat
     8701 cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg
     8761 gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttgattgc tctttatagc
     8821 tatatggtca ggcgcttgct gcaagccaat ttacgcaacg atgtctccgc agtcgaagaa
     8881 gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact ctccccttct
     8941 ttgattcagg acccagtctg atgaaccatg caccgcattt gtatttcgcc tggcaacaac
     9001 tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca
     9061 tcgccagcga aatggcgtat gtgaatgcgg tgcaggagat tgcacatttg actgaagagg
     9121 ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgcgcctg attctcgaca
     9181 acgaaagcaa ggtaaagcag ttattacaga ttcgaatgga tgaactggcg aaactggtcg
     9241 gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc
     9301 tggctccgca ggataacctc ttttgaatct gaatgagtcg atggctcgcg aataatccga
     9361 ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta atcccacgct
     9421 gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga
     9481 gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa
     9541 aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg atttaggcga
     9601 gtttgatcag aaaggcagca tccctactaa atatggcgat aaagcacaac tgctggccgc
     9661 cattgacgcc ctgaaacgta atgacattgc ggtgctgttg gacgtggtag tcaaccacaa
     9721 aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg acgaccgtac
     9781 gcaaattgat gaagaaatca ttgagtgcga aggctggacg cgttacacct tccccgcccg
     9841 tgccgggcaa tactcacagt ttatctggga tttcaaatgt tttagcggta tcgaccatat
     9901 cgaaaaccct gatgaagatg gcatatttaa aattgttaac gactacaccg gcgaaggctg
     9961 gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg agaatatcga
    10021 tttccgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca
    10081 aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccggcgt ggttttataa
    10141 agagtggatc gaacacgtac aggaagttgc gccaaagccg ctatttattg tggctgaata
    10201 ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat
    10261 gctgttcgat gcaccgctgc agatgaaatt ccatgaagca tcgcgcatgg ggcgcgacta
    10321 cgacatgacg cagattttca cgggtacatt agtggaagcc gatcctttcc acgccgtaac
    10381 cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg tcgaaccgtg
    10441 gtttaaaccg ctggcgtatg ctctaatttt gttgcgggaa aatggcgttc cttcggtatt
    10501 ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc aaacctatcc
    10561 gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc
    10621 ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta gccgcagtgg
    10681 cactgacgaa tatcccggct gcgtggtggt catgtcgaac ggggatgatg gcgaaaaaac
    10741 cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca
    10801 agagagcgta ataaccgacg aaaacggcga agcaaccttc ttttgcaacg gcggcagtgt
    10861 cagcgtgtgg gttatcgaag aggtgattta aattcatccc cggcggcaag ccggggagat
    10921 ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg
    10981 tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct
    11041 caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca
    11101 acttacccgg cacggcaatc acccgctgcc actgacggca atccagagta tcgcccgctt
    11161 tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg
    11221 tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt
    11281 cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca
    11341 ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt cgccagcctt
    11401 tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta ccccgaaaat
    11461 aaatttgcat ggtgtatgat tcgcgactct tgtttcaaac cttctgagtt cagaggctac
    11521 attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg cgcccatccc
    11581 cgcggtcatc gcggcgggta ttctctccac ttactatttt ggcatcactg gcaccttttg
    11641 ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt tcggcgtcca
    11701 tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccgcat
    11761 cgacgggatg atgatcctcg gtatgtttgg cggctgcttt gccgcagcgc tgtgggccaa
    11821 caatgtcaaa ctacgcatgc cgcgtagccg tatccgcatt atgcaggcca tcattggcgg
    11881 cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg cgttctttac
    11941 cggtattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg ccattggttc
    12001 atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga aaatgcagaa
    12061 agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcgacgtc gttttcgtct
    12121 cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc
    12181 caaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg aacgtgcgca
    12241 aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa
    12301 agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg tacagttagg
    12361 cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt tactgtttgg
    12421 ttttggcatc gtgctggctg gcggctgcga aaccggctgg atgtaccgcg cggtagaagg
    12481 ccaggtgcac tactggtggg tcggtctggg caatgtgatc ggctcaacga ttctggcgta
    12541 ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa
    12601 aacctttggc ccgatgggtg gcctgctggt gacatatttg ctgttgtttg ctgcgctgat
    12661 gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga ctgctaagga
    12721 gatcgcatga aaaatatcgt tcctgattac cgtcttgata tggtgggtga accctgccct
    12781 tatccggcag tcgcaactct tgaggcgatg ccgcagttaa aaaaagggga aatcctggaa
    12841 gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg taatcacggc
    12901 tatacggtgc tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa
    12961 tccctcattc ccgctgtgac ccacagcggg aatatcgttg cctctgtacc acatagattt
    13021 tacccgtccc ctctcctatt cttttcagtg tgctctcagg aggtttctat gtgtggtcgc
    13081 tttgcccaat cccaaacgcg tgaagattac cttgcgcttc tcgctgaaga tattgaacgc
    13141 gatattccct atgatcccga acccattggc agatacaacg tcgcgccggg aaccaaagtc
    13201 cttctgctca gtgagcgtga tgaacacctt catctggatc cggttttctg gggatatgct
    13261 cccggatggt gggataaacc gccgctgatt aacgcccgcg tagaaactgc ggcaaccagt
    13321 cgtatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt
    13381 gagtggaaaa aagaaggcga caaaaagcag ccttatttta tctatcgtgc tgatggacaa
    13441 cctgttttta tggcagcgat aggcagcaca ccgttcgagc gcggtgacga agccgaagga
    13501 tttttgattg tcactgcagc agcagaccag ggtctggtag atattcatga ccgccgcccg
    13561 ctggttctgt cgccagaagc tgcgcgggaa tggatgcggc aagagattag cggtaaagaa
    13621 gcctcagaaa tcgcggccag tggctgtgtt ccagcaaacc agttctcctg gcacccggta
    13681 tcgcgcgcgg tgggtaatgt taaaaaccag ggtgcggagt taattcaacc tgtttgataa
    13741 gcctggagat tattgatcaa caatactgcg tcataagtaa tctctattag acaaagattt
    13801 cattacctgt tggcatattg caaaaataac accaatacgg aatcgtcatg ttcacgatta
    13861 aaacagatga tctcacccat ccagcagtgc aaacattagt ggcttaccat atttccgaca
    13921 tgctgcagca gtctcccccc gaaagcagtc atgctttaga tgtgcaaaaa ttacgtaacc
    13981 cgacagtgac attctggtca gtatgggaag gcgaacaact cgcaggaatg ggtgcgctga
    14041 agttgctgga tgataaacat ggcgagctaa aatcaatgcg taccgcgcca aattatttac
    14101 gtcgtggtgt cgccagtctg attttacgcc acattttgca ggtcgcccag gacagatgcc
    14161 ttcatcgcct gagcttagaa acgggtacac aggctggatt tacggcctgc catcatattt
    14221 atttgaagca tggtttcgtt gattgcgaac cgtttgctga ttatcaactt gatccacaca
    14281 gtcgattttt atcattgacg ctatgcgaaa ataatgagtt gctatgagcc agacgcagca
    14341 cattctt
//
LOCUS       DAZGCQ010000117        14341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000117 DAZGCQ010000000
VERSION     DAZGCQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.117"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..393
                     /locus_tag="ACHK07_004094"
                     /pseudo
     CDS             106..393
                     /locus_tag="ACHK07_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            837..2093
                     /gene="espR1"
                     /locus_tag="ACHK07_004095"
     CDS             837..2093
                     /gene="espR1"
                     /locus_tag="ACHK07_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO8104366.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNALESINFPQGRNVSITHISMNNNCLRNIDIDRLSSITYFSAAHNQLE
                     FVQLESCEWLQYLNLSHNQLTDIIAGNKEELLLLDLSHNKLTSLHNALFPNMNTLLIN
                     NNLLSEIKMFYSNFCNVQTLNAANNQLENINLHFLTYLSSIKSLRLDNNKITRIDTNN
                     TSDIGTLFPIIKQSKNLNFLNVSGKNNCPTIQLMLFNLFSPALKLNTGLAILSPGAFE
                     DHSDGSDVDNELFHYTINKAYTPYNIHTYKTEEVVNQRDIKIKNMTLDEINNTYCNND
                     YYNQAIREEPIDLLDRSFSSSSWPFE"
     gene            2413..3801
                     /gene="narU"
                     /locus_tag="ACHK07_004096"
     CDS             2413..3801
                     /gene="narU"
                     /locus_tag="ACHK07_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO8104367.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            3896..7636
                     /gene="narZ"
                     /locus_tag="ACHK07_004097"
     CDS             3896..7636
                     /gene="narZ"
                     /locus_tag="ACHK07_004097"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO8104368.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7633..9177
                     /gene="narH"
                     /locus_tag="ACHK07_004098"
     CDS             7633..9177
                     /gene="narH"
                     /locus_tag="ACHK07_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8104369.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            9177..9872
                     /gene="narW"
                     /locus_tag="ACHK07_004099"
     CDS             9177..9872
                     /gene="narW"
                     /locus_tag="ACHK07_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8104370.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            9869..10549
                     /gene="narI"
                     /locus_tag="ACHK07_004100"
     CDS             9869..10549
                     /gene="narI"
                     /locus_tag="ACHK07_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8104371.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10629..11522
                     /gene="yddE"
                     /locus_tag="ACHK07_004101"
     CDS             10629..11522
                     /gene="yddE"
                     /locus_tag="ACHK07_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO8104372.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEDETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(11618..12463)
                     /gene="nhoA"
                     /locus_tag="ACHK07_004102"
     CDS             complement(11618..12463)
                     /gene="nhoA"
                     /locus_tag="ACHK07_004102"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO8104373.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALRLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            12636..13205
                     /gene="yddH"
                     /locus_tag="ACHK07_004103"
     CDS             12636..13205
                     /gene="yddH"
                     /locus_tag="ACHK07_004103"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO8104374.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(13209..13436)
                     /gene="pptA"
                     /locus_tag="ACHK07_004104"
     CDS             complement(13209..13436)
                     /gene="pptA"
                     /locus_tag="ACHK07_004104"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO8104375.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(14031..>14341)
                     /locus_tag="ACHK07_004105"
     CDS             complement(14031..>14341)
                     /locus_tag="ACHK07_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104376.1"
                     /translation="NIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDATP
                     NIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTDKRPALGY
                     "
BASE COUNT         3659 a         3579 c         3462 g         3641 t
ORIGIN      
        1 tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat
       61 gtctcccggc aagaatctct gattgagtta agtgcccatc acaatatgat cactgacctt
      121 atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat
      181 ttaaacacca aaaccactac aaaacttgaa tatttaaact taagcaataa caatttattg
      241 ccaaccaatg acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac
      301 ggcatcaaca atgattcact tgcacaaatg cagtactgga ctgcagtaag aaatataatt
      361 gatgacacta atgaagtgac cattgattta tcttataacc tggcaatcac aaatatcgat
      421 accagtgatg aacatcttgc agaaataagc gataattcag aaggtaatta tataaaagac
      481 aatgactcaa tgtctattcg ttatagatca aaatattatt ccagagattc cgccttaata
      541 gaagaagaaa caatattttc tgacgcagaa ctaaaagcta ttctgcctat gcgtcgcatg
      601 tacggggttg gtgactataa gtcaaattcc tcttctctat cctcactctc ggggctcaag
      661 gacccaacgg gcacacccgt ctgttattat attcataatg agagtaaacc ttccttaggt
      721 tatggtccaa catgcaataa ttggttaagc caatccttta caacagagtt ataaaacaca
      781 tcctgattta tcaagggtga taaatcagaa aaacattgta ttactacgga gcagttatga
      841 aattcccttc aatatttaac aaaataaaac cacaatccat acagcaacat ccagaaaaaa
      901 atcaacttaa ctggatgctc gaattaaata aatggaaaga agaacgtata cttacaggtg
      961 aaatccatcg tccggaatgt cgaaacgaag ccgctaaaag gataaactgt gcttttttgt
     1021 cgaaacagaa tgacattgat ttatcaggac ttaatttaac tactcaacca ccagggctgc
     1081 aaaacttcac ctctatcaat cttgataata accaactcac acattttgat gcaaccaact
     1141 acgatagact cgtaaaactt agtctgaata gtaacgctct tgagtcaata aattttcctc
     1201 aaggcagaaa tgtaagcatt acacatatat ctatgaataa taattgtctc agaaatattg
     1261 atatagatag gctttcatca attacttatt ttagtgcggc acataatcag ctagagtttg
     1321 tgcaattaga atcttgcgaa tggctgcaat acctgaatct cagccataat caattaactg
     1381 atattatagc aggaaataaa gaagaactct tactgctgga tctatcccat aataaactaa
     1441 caagtttaca caatgcctta tttcccaaca tgaatacgtt acttatcaac aacaacttgc
     1501 tttctgaaat taaaatgttt tatagcaact tctgcaatgt tcagacatta aacgctgcta
     1561 acaatcagtt ggaaaacata aaccttcatt tcctgactta tctttcatct atcaaaagtt
     1621 taaggctgga caataataaa ataacccgca ttgatactaa taatacatcc gatattggaa
     1681 ctttattccc cataataaaa cagagcaaaa acttaaattt tttaaatgtt tctgggaaga
     1741 acaattgccc tactatccag ctcatgttat ttaatttgtt ttccccagca cttaagctta
     1801 atactggcct ggcaattctt tcgcctggag catttgaaga tcactctgac ggatcagatg
     1861 tggataacga attgtttcac tatactatta ataaagcata taccccatat aatatacata
     1921 cttataaaac agaagaagtt gtaaaccaga gggatataaa aattaaaaat atgaccttag
     1981 atgaaataaa caatacttat tgtaataacg attattacaa tcaggcaata agagaggaac
     2041 cgatagacct tctggacaga tcgttttcct ccagctcatg gccttttgag tgaataaatt
     2101 attgctaatt ctataagtgt cttgtcttgt taagaatata gttaatatac aaccctttgg
     2161 attcatcatc tgaagggttg aatcatgaca tgcgtacata gctgaacccc gtttaatggt
     2221 tagcttgtta gttacattta gtaacacata actggctgct taatccccat tctgcccccg
     2281 cggttacgct ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct
     2341 aataactggc tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat
     2401 gtgaggaaca atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg
     2461 aaaccagaaa atccggcctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc
     2521 tggatatcag tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt
     2581 accgttaatc tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc
     2641 gcattaccct ccgtttctgg cgcattattg cgtgttccct actcctttat ggtacctata
     2701 ttcggtggac gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg
     2761 ctcggaattg ccgtgcagaa tccgaatact ccttttggga tctttatcgt tatcgctttg
     2821 ctatgcggtt ttgcaggtgc gaactttgct tcgagcatgg gcaatatcag tttcttcttt
     2881 ccaaaagcca ggcaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta
     2941 agtgtgatgc agctggttgc accgctggtc atttttgtac ccgtattcgc ctttctcggc
     3001 gtcaatggcg tcccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt
     3061 tgggtaccgc tactggcgat tgccacgatc gccgcctggt cagggatgaa tgatatcgcc
     3121 agttcacgcg cgtcaattac cgaccagctc cctgtcttac aacgcctgca tctctggctg
     3181 ctgagcctgc tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc
     3241 atgctggcaa aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca
     3301 tttatcggtg ccatcgcgcg gtcggttggt ggtgctattt ccgataaatt cggcggcgtg
     3361 cgggtgacgt tgatcaactt tatttttatg gcgattttca gcgccctgct gttccttacc
     3421 ttaccgggca caggctccgg taatttcatc gcattttacg ccgtatttat ggggctgttt
     3481 ctgaccgcgg gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag
     3541 ataaccattt atcgggtgaa gatgaaaggc ggtagtgatg agcaagctca gagagaagct
     3601 atcaccgaaa cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt
     3661 tttattccgc aggcatttgg catgtcgctc aatatgaccg gctctccagt gggcgcgatg
     3721 aaagtgtttt taatcttcta catcgtttgt gtgctactga cctggctggt ttatggtcgg
     3781 cggaaattca gccaaaaata agagatcgat gcctgacacg tcttatcagg ctacaaccgt
     3841 gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag
     3901 taaacttttg gatcgctttc gctacttcaa acaaaagggc gatacctttg ccgatggtca
     3961 cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt
     4021 cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat
     4081 ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg
     4141 ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta
     4201 tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg
     4261 gcgcgaagcc ctcaaacaac acagcgatcc ggtacttgcg tgggcatcga ttatgaacga
     4321 tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa
     4381 ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca aaacctacgg
     4441 cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt cttacgccgc
     4501 cggaacacgt tatctgtcac tattaggtgg cacctgttta agcttctacg actggtattg
     4561 cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtgc cggaatctgc
     4621 cgactggtat aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac
     4681 gccggacgcc catttcttta ccgaagtacg ctacaaaggc actaaaacca tcgccattac
     4741 ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccaa aacaaggcac
     4801 tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa
     4861 tcccagcgac tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat
     4921 gctggaacct cgcgacgacg gtagctacgt acccgggcgc atgatccgcg cgtctgacct
     4981 ggtagatgga ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc
     5041 aggtgaattg gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg
     5101 gaatctggaa tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca
     5161 acatgacgcc gttgcaggcg tggctttccc ctactttggc ggcatcgaaa atccgcattt
     5221 tcgcagcgta aaacacaatc ctgtcctggt gcgtcaatta cccgttaaaa acctgacatt
     5281 agccgatggc agcacctgtc cagtggtcag cgtttatgat ttggtactgg cgaattacgg
     5341 cctcgatcgc gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta
     5401 cacccctgcc tggggcgagc aaattaccgg cgttccgcgc cagtacattg aaaccatcgc
     5461 ccgcgaattt gccgataccg ctcataaaac gcatgggcgc tcgatgatta tcctcggtgc
     5521 tggtgtcaac cactggtatc acatggacat gaactaccgc gggatgatca acatgctgat
     5581 cttctgcggt tgtgtcggac aaagcggtgg cggctgggca cactatgtcg gtcaggaaaa
     5641 actgcgcccg caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgaccacc
     5701 acgccagatg aacagcacct cgtttttcta caatcattcc agccaatggc gctatgaaaa
     5761 agtctctgcg caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct
     5821 gattgatttc aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg
     5881 gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt
     5941 taccgcccag gcgctgaaat cgggcgagtt gcgtatggcc tgcgaacaac cagatagcgg
     6001 cagcaatcat ccgcgtaatt tgtttgtctg gcgttctaac ctgcttggct cctccggcaa
     6061 aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcaagga
     6121 actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga
     6181 aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc
     6241 cgatatcgtt ctgcccaccg ccacctggta tgaaaaagac gatatgaaca cctcggatat
     6301 gcatccgttt attcatccgc tatctgcggc ggtcgatccg gcctgggaat cacgcagcga
     6361 ctgggaaatc tacaaaggta ttgccaaagc attttcgcaa gtgtgtgtgg gtcatcttgg
     6421 caaagaaacc gacgtggtat tgcaaccact gctgcacgac tctccggcag agctctcaca
     6481 gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gtaaaaccgc
     6541 gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct
     6601 cgggccattg atggacaaac ttggcaacgg cggtaaaggg atttcgtgga atacccagga
     6661 tgaaatcgat ttcctcggta aactcaatta caccaaacgt gatggcccgg cacaggggcg
     6721 tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccagaaac
     6781 caacggtcat gttgcagtta aagcgtggca ggcgctgggc gagatcaccg gacgcgaaca
     6841 tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca
     6901 gccgcgtaaa attatctcca gccccacatg gtctggtctg gaaagcgatc acgtctccta
     6961 taatgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca
     7021 gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg
     7081 cccgcctatc gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc
     7141 ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag
     7201 tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac
     7261 cgatgcccgt gaactaacca ttgtcgataa cgactgggtg gaagtattta acgccaacgg
     7321 cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga ccatgatgta
     7381 tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg
     7441 cattcataac tcggttaccc gcgtttgccc gaaaccaacg catatgattg gcggttacgc
     7501 gcagctggcc tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat
     7561 catgatccgc aaaatgaaaa acgttaactg gctggatgat gaaggtcgcg atcaggtaca
     7621 ggaggcgaaa aaatgaaaat ccgttcgcaa gtcggcatgg tgcttaatct cgataaatgt
     7681 atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccggtcg cgaaggtatg
     7741 gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg
     7801 gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg
     7861 cgtctgggca gcaagatggg tgtaataacc aaaatcttcg ccaacccggt ggtgccgcag
     7921 attgatgatt actacgaacc tttcacattc gactacgaac atttgcatag cgcaccggaa
     7981 ggcaaacata tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa
     8041 gtgatctggg ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc
     8101 gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa caccttcatg
     8161 atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gctgcgtggc gacctgccca
     8221 agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc
     8281 cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa
     8341 agcggcaagt cagaaaaatg tatcttctgt tacccacgaa ttgagtccgg tcaaccgacc
     8401 gtgtgctcag aaacctgcgt gggtcgcatc cgatatctgg gcgtgctgct ttacgacgcc
     8461 gaccgcattg aggaagcggc aagcaccgag cgcgaggttg acctctatga acgtcagtgc
     8521 gaagtgttcc tcgatccaca cgatccctca gtgatcgaag aagccctgaa acaaggtatt
     8581 ccacaaaacg tgattgacgc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg
     8641 aaactggcgc taccgttgca ccctgaatac cgcaccctgc cgatggtctg gtacgttcct
     8701 ccgctgtcac cgattcagtc ttacgctgat gcgggcggtt tgccgaaaag cgaaggcgtg
     8761 ctccccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc
     8821 ggtgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg tcactatatg
     8881 cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc
     8941 gtcgcccagg tcgaagagat gtatcgctat ctcgccattg ccaactatga agatcgtttt
     9001 gttatcccga ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc
     9061 ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt
     9121 agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc
     9181 agatcctcaa agtgatcggt ctgttgatgg agtatccgga cgagctgttg tgggaatgca
     9241 aggaggacgc gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca
     9301 acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg
     9361 ggcgcactac gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc
     9421 aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg
     9481 aactgcccga ttatctaccg ctgtatctgg agtatttaag catactgcct gacgatcagg
     9541 cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac
     9601 aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtacac
     9661 tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg
     9721 cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt
     9781 gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt
     9841 atgtcgacat cagtgcggga ggtgagaaat gattcagtat ctgaacgtct ttttttacga
     9901 tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta
     9961 cgggcagtac acctggcgcg cctcctcaag tcagatgctc gataaacgcg ggatggtgat
    10021 atggtcgaat ctgttccata tcggcatttt ggggatcttc ttcgggcacc tgttcggcat
    10081 gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactaatggc
    10141 gatggtgctc ggtggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg
    10201 gcgcaggctg acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat
    10261 gagcattttg ctggtccagt gtctgttggg gctaagcacc ataccgtttt cagcacagta
    10321 tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg
    10381 tggtggatcg tcagagatgc ttaacggtgt agcgttcgtt ttccgcctgc atctggtgtt
    10441 gggaatgacc atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc
    10501 gtttgagtac ttcacccgcc ggtatcagat tgtgcgctcg cggcgataat tttcaccctg
    10561 gccggatagc ccttcgctct ccggccactt gcaggctatg atagttctcc agccacagga
    10621 gaacgttaat gaaaccgcag gtttaccacg tcgatgcttt tacctctcaa ccatttcgcg
    10681 gcaattctgc cggtgttgtt ttccccgccg ataatcttag cgaagcgcaa atgcagctta
    10741 tcgcccgcga attaggtcat tcggaaactg cttttctgct gcacagcgac gacagcgatg
    10801 tgcgcatccg ctactttacg ccaacggttg aagttccgat ttgtggtcac gcgacagtag
    10861 ctgcgcacta tgtacgtgcg aaggtgttag gtttaggaaa ttgcacggtc tggcaaacat
    10921 cgctggcagg aaaacatcgc gtgactatcg aaaagcacca cgatgattat cgtatttcgc
    10981 tggaacaagg tacgccgggc tttgagccac cgctggaaga tgaaacacgt gcggcgatta
    11041 tcaacgcgct tcatctcact gaggacgata ttctgccagg cttgccaatt caggtggcaa
    11101 caacaggaca ctcaaaagtg atgatcccgt tgaaaccaga agtggatatc gacgcccttt
    11161 cgcctgatct tgctgcgctg agcgctatca gcaaacagat tggctgcaat ggtttcttcc
    11221 cgttccagat ccgcccaggc aaaaacgaaa ccgatggtcg catgttctcg cctgcgattg
    11281 gtattgtgga agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac
    11341 atcacaacgt attgccccac gatggcaagg tgctgcgtgt taaaggccat caggggcgcg
    11401 cattggggcg tgacggcgtg attgaagtga cggtgacaat tcgtgataac caaccggaaa
    11461 aagtcaccat ttctggcgcg gcggtgattt tgtttcatgc cgaatgggca attaaactct
    11521 gaaaccgttg cctgatgcgc ttcgcttatc aggtctacaa ccgagctaat gtatgccgga
    11581 taaggcgttt acgccgcatc cggcaacctg acataaatta ttttcccgcc tccgggtgcg
    11641 tatcaaacgc cgccatcacc agcgctaact catccacggt aaagccatgt ttcgcatcat
    11701 ccacgcccag accaaactgt tcttgcatca cagcatataa tgacgccaca tccggtagat
    11761 ttcgctgctc caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca
    11821 gcttgccgcc gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg
    11881 gccagtgcgc cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat
    11941 caaaacggta catcgactgc caatgctgat gatgattaaa ctgcaacacc caatcatcac
    12001 cctcctgcaa caaccgatac tctccgtgtg gcgtggtctg cacgagatcg ggaactaaac
    12061 gaatcggcgc ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt
    12121 ccagttcaac caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca
    12181 ctacccgccc taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca
    12241 cgccattctg ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg
    12301 attgatcatc aagctgtatt tccctcggca gcaaaacgtc gaggttttca aacggaatgg
    12361 tgcaattgtg tttcaggcgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg
    12421 accagttaat acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt
    12481 ggttttctca acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga
    12541 catgcatcac gttcgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac
    12601 tacgcttaaa aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat
    12661 tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg
    12721 agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac
    12781 ctccacgcgt ggcgattgtg gtagataaat cgacatggac cagggagttg attgaacata
    12841 acggtaaatt tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcagtgg
    12901 gaagtgtgtc ggggcgtgaa gaagataaat ttaattgcta tggcattccg gttgtgagag
    12961 gcccggtatt tggtttgcct ctggtcgagg aaaaatgtct ggcgtggatg gagtgtcgcc
    13021 tgctacctgc gacttctgcg caagaacaat acgacacact gtttggcgag gtagtatcag
    13081 cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca
    13141 atacgttgca tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcgg
    13201 gttaagcatt acatgctgta accaggtttc tttatcaaag cctccatttg gggcgcgatt
    13261 tcggcatccc agatagcttg ccatgattct ggttgaatct gctgtagaga aatgcttatc
    13321 gagctatctt tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct
    13381 gctttttgtt gttcgtccag ttcacgcgga aaacatttaa tgtcgatgtg cggcatgatt
    13441 gtgctccttt tgaaagatgt cagtaaaatt aacacacatt gtgaatgtcg tatgtccggt
    13501 gatgcgtttt tacgacaata atgcctactt gaaattgtag atatttgcac tacacccttt
    13561 taaataaagc accaacaaat cgtcaacttg cagaacatat tcttctgcct acataccatt
    13621 aagaaaacat caagttcccg acttgccaat ttattaacgc ccattataga cgctgaaaca
    13681 agctacggac aaaggagatg ccaaaattac cggcaatgca gaaaaactca acgaaccatg
    13741 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
    13801 gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat
    13861 ctttgtgcct acggcacaca gtgatctttc cctggtccct gtcccggatt gtagtaccgg
    13921 tgccagttgt agtgcagttc ggattcgtcg tcatactgct agcccggatg gcggagcagt
    13981 tgctctagtc actcagaata ttgtaatagt gatagagtgc agtgccggtt tcaatatcct
    14041 aacgctggtc ttttgtctgt attaactgat ttagctggtt tgctacatat taatgccttc
    14101 agattttccc tttccttgtc tgtgatactg tcgcaaaccc tactagccca ttctacttca
    14161 ctcttctcca ggcattctat tttcttattg ctcattgttc taatgtttgg tgttgcatct
    14221 ccatttaata acatccatgt ttcatttttc ttcataaaac atcttccatt atgttcaaag
    14281 atattaggtg tgaatatatt atcagaatcc catgttacga cttgctcgag ggctatatta
    14341 t
//
LOCUS       DAZGCQ010000118        14268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000118 DAZGCQ010000000
VERSION     DAZGCQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.118"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHK07_004106"
     CDS             <1..297
                     /locus_tag="ACHK07_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8104377.1"
                     /translation="LSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRG
                     WFIARGFRAERIHATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGETSVTD"
     gene            complement(466..933)
                     /locus_tag="ACHK07_004107"
                     /pseudo
     CDS             complement(466..933)
                     /locus_tag="ACHK07_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(982..2276)
                     /gene="insG"
                     /locus_tag="ACHK07_004108"
                     /pseudo
     CDS             complement(982..2276)
                     /gene="insG"
                     /locus_tag="ACHK07_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2390..2965)
                     /locus_tag="ACHK07_004109"
     CDS             complement(2390..2965)
                     /locus_tag="ACHK07_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8104378.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFSGGLSALASQHGVNV"
     gene            complement(3033..3611)
                     /gene="terD"
                     /locus_tag="ACHK07_004110"
     CDS             complement(3033..3611)
                     /gene="terD"
                     /locus_tag="ACHK07_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO8104379.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(3660..4700)
                     /gene="terC"
                     /locus_tag="ACHK07_004111"
     CDS             complement(3660..4700)
                     /gene="terC"
                     /locus_tag="ACHK07_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO8104380.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            complement(4719..5174)
                     /locus_tag="ACHK07_004112"
     CDS             complement(4719..5174)
                     /locus_tag="ACHK07_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8104381.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            complement(5197..6354)
                     /locus_tag="ACHK07_004113"
     CDS             complement(5197..6354)
                     /locus_tag="ACHK07_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8104382.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6354..6935)
                     /locus_tag="ACHK07_004114"
     CDS             complement(6354..6935)
                     /locus_tag="ACHK07_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO8104383.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            7255..8313
                     /locus_tag="ACHK07_004115"
     CDS             7255..8313
                     /locus_tag="ACHK07_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO8104384.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            8323..9465
                     /locus_tag="ACHK07_004116"
     CDS             8323..9465
                     /locus_tag="ACHK07_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO8104385.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            9458..10231
                     /locus_tag="ACHK07_004117"
     CDS             9458..10231
                     /locus_tag="ACHK07_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104386.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            10233..11312
                     /locus_tag="ACHK07_004118"
     CDS             10233..11312
                     /locus_tag="ACHK07_004118"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO8104387.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            11312..12268
                     /locus_tag="ACHK07_004119"
     CDS             11312..12268
                     /locus_tag="ACHK07_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO8104388.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            12279..13374
                     /locus_tag="ACHK07_004120"
                     /pseudo
     CDS             12279..13374
                     /locus_tag="ACHK07_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13392..13859
                     /gene="terW"
                     /locus_tag="ACHK07_004121"
     CDS             13392..13859
                     /gene="terW"
                     /locus_tag="ACHK07_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO8104389.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
BASE COUNT         3553 a         3698 c         3546 g         3471 t
ORIGIN      
        1 ctttcccgtg tggcagagaa gtttcaggcg ggtcatgttg tcggggtgaa gatagaagga
       61 tttacggacc atcttggcgg gcaggcgtat aaccggctcc tgtcagagaa acgggcggaa
      121 tcagtcaggg ggtggtttat tgcccgggga ttcagggctg aaaggataca tgcaacgggc
      181 cgtggatatg atgcaccagt ggtgttctgc ccggatgtgg agggcgaagc actgaaggaa
      241 tgtctgcgcc ccaaccgcag ggttgaaata accggggaaa catcagtgac tgattaacaa
      301 taaagtgccc cgggagctaa tgccagtcag ttcagcaact gactggctct ttttcggggc
      361 tgtggggtat ttccagggag cctgtttagg aatctgtgta aatgcctttt ctcagaagtg
      421 accgtccagg cggtcaccga actcgataat aaagcggctc attgccattc gccggttttg
      481 ccattctaca acctgctcca tcacggcatc ggtgaccttt gatatcagtg ccggtgaaac
      541 atctgcgtca tacagctctt tgaacgcagc agcgatctca cgggtggtca tccctttggc
      601 atacaacgag aagatctggt tatccatccc agtaatacgg gtctgatttt tctttaccag
      661 ttgtggttcg aaggtaccgt cacgatcgcg cggagtacgc agttccagtg gaccgtcgcc
      721 tgtgataacg gtctttgtgg aataaccgtt gcgggagtta gttcccggtt tggactgatt
      781 tttctcatac ccaagatggt gtgtcatctc tgcattgaga gcggcttcaa cgctgatctt
      841 tttcagtaga cggtcaaact ggctgaggtc ttcaggggtt ttgaggtttt tggccagttc
      901 gttagccaga gcctgtaact gtttttcgtc cataaattaa ccttcatttg atgcttgatt
      961 gaacatatca aaatcaggca attacacaaa tctatgtaca ggctctttcc aaggcctctc
     1021 ctttaccact ctcgggaagc ccctttccct tcttgtcggt aatttcacaa gttgtcccat
     1081 acttgcaaga tcgcgcatca gccccggtat acgtcccggt gaagcgccct gcaatgtcat
     1141 caccattccg catgattctg agaaactcag ttgattcggc cagtaacctt tcagatgttc
     1201 cgccatttta atcatctgac atcttaccag attataagcc agtaagacac cccacagctc
     1261 ttgctccaca agctccggct ttttacagcg tcagcctgct cagttgcatc gtctgtttta
     1321 tctccctgta tcccagttca atttcccggc gatgactgta cagatccgcc atttctcccc
     1381 cggggaagcg catggcgtcc gtcatcgacg tcagcagatg gcagacttct cctttgcgcg
     1441 tcacggtcag caggcgggct gtcacctcat ttcccagtcc cggccacttt tttcgtgcct
     1501 gcgggctggt tttcagcttc accagatgat cgcctttacc cagttttctg atctcttcat
     1561 attgcgctcc ctttctgaga gggatcatcc agtggcggtg ttctcccacc aggctccagg
     1621 catttaacag tcccagtgag taataacctt tatccattaa cgtcagggtg ttatcgccgg
     1681 tttgttctat aagttgctca gcaagctcat tttcgctgtt cttcatcgtg ccgaaggctc
     1741 agccgtaagc agatggctgg tcagttccat ctggcagact attttgacct gcgggtagag
     1801 cgccgggttg ccggcatgta tctggcgggg gaaggctgca tcgttctctg gtgtatccgg
     1861 tgtgcgccag aacataccat cgatggccag cagggtcagg ccgcatcagt gcggatgcgg
     1921 cgtggcgtta tgccagagct gcgctgtttt cgtgaacacg cggcggacag cctcacttcc
     1981 caggtgctgg cgggcctgaa taacggcact gggggcaacg aaggggcgat tgccggcagc
     2041 atgatgtcca ggcgattcac aatctggtga aggggctctt tacgctcaag cgccatgcca
     2101 acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt taccgtacct
     2161 gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag agaagccagt
     2221 gggttacgca gagaatcgta acgggatacc agatcaaggg cctgtccagt gtgcataaaa
     2281 aatccggaaa cgagtgagcg tttccggatt cttacacaac cactggatcg gtcaactgat
     2341 ccttaaccga tcggcattac ggcattgccg gggtcagact aagcatcttt tagacgttga
     2401 cgccgtgctg ggaagcaagc gctgacaggc caccggaaaa accctgaccg acagctttaa
     2461 acttccactc agcgccatgg cgatacagtt caccgaagac cattgcggtt tcagttgagg
     2521 cgtcttcaga cagatcgaaa cgggcaattt ccgcgccgtt atcgttgttg taaacgcgca
     2581 tgaagctgtt gctcaccatg ccgaaatttt gtttacgcgc ttctgcatca tagatggtaa
     2641 cggcaaatac cagttttttg acgtcaactg agacttttgc cagatcgatt ttaatttgct
     2701 catcgtcacc atcgccttca ccggtacggt tgtcaccctg gtgctctact gcgccatcag
     2761 ggctggtttt attattgaag aaaatgaaat gggcatctga cagcacttta ccgtcttcac
     2821 ctactgcgaa tacggaagcg tccagatcaa aagcctgacc gtcagttaca cgggcatccc
     2881 agccaagacc aaccatagca acattcatgg tcggtgcttc tttggtcaga gatatgttgc
     2941 cgccttttac gagagaaact gccattttta actcctgcaa acagttgaat gagggctgaa
     3001 tcaacagccc ggaatgaaaa gttagttttc gatcaagacg cgttaatgcc gtactgagca
     3061 catacagatg ccagcccccc cgcataaccc tgacctacag cacggaattt ccactcacca
     3121 ttgtgacgat acagttcacc gaacagcatg gcggtttcag tggacgcatc ttcagtcaga
     3181 tcgtagcgag caacttcagt ctggttatcg tcattaacca ggcgaataaa cgccccggaa
     3241 acctgaccaa agttctgacg gcgagcctga gcatcatgga tcgtcacaac gaagatgatc
     3301 ctgtcgatgt cagacgggac agtatcaagt ttaattttca gcgattcatc atcaccatcc
     3361 ccctcaccag tacggttatc gccggtgtgc gttacagaac cgtcggatga cgtcaggttg
     3421 ttatagaaga tgaaatctga atcgccacgc actttgccgt ttgcggtcag caggaatgct
     3481 gaagcatcca agtcaaagtc ctgaccgtca gttgaacgtg catcccagcc aagacccacc
     3541 aggacgtttt tcattgacgg agctgcttta ctcagggaga cgttcccgcc tttggaaaga
     3601 gaaacactca taaaataacc tcttcgatta gtaattgctc cggttaacac ttaaggggat
     3661 cagttcccct tttcctcaga ttcaggtttg ccggggaaaa taacgcttgc gagaatgccc
     3721 agcaccagaa cccccagtac aacatacagg ctggttgtcg cagcgatgct gtaaccatga
     3781 tgccagatgt gatcggtcgc gttcaggccc agttttaccg caatgaagaa caacaacacg
     3841 ataacagcct tttccagatg aatcaggtac tgtttcagtg cttcaaggac aaagtacaga
     3901 gtacgcagcc ccaggatagc aaacatcatg gcactataga cgataagcgg ttcacgactg
     3961 acagcaatga ttgccggtac cgagtcaaac gcaaacataa catccgagag ttcaaccacg
     4021 gccacacaca ggaacagagg ggtcgcatac agcgccgcct ttttaccacg gtcaatggtt
     4081 acatcactgt tttctggttt cgccagttca gcatccactt ccttctggtt aagcaggaag
     4141 gcatgcccac gtagcttcgg ccagacaggg aagaaacgtt taaccatgcg gtaagccaga
     4201 tgctgggagt aatcttcaat ttcatcatca tcatcaccgc ttttaagcat catgaccgct
     4261 gtccaggcaa cgatgatagc gaagacgact tcaacgtacg gccccagact cagcaggctc
     4321 gtaccgatgg cgacaaagat tcccctgaag acgatcgctc caatgatccc ccagtaaagc
     4381 acacggtgcc gataacgatc cggaacagcg aaccaggaaa agatggccat catgacaaac
     4441 aggttgtcga ccgacagtac tttctccagc gcataccccg tgacaaacaa actggcaact
     4501 tcagcaccgt ggtggatata gaggaaaccg gcaaatgcca tcgcaacaac aacccagaat
     4561 acggaccaga gcgccgcact cttcagggag attggcttgt catcacggtg cataaacagg
     4621 tcaataaaga tggctccgac tgaaagagca ataaaaacaa tgaccgtttc agtcgggaag
     4681 ccgatgtgtg tggaaaccat attttaccct tagggatgtc agaggccaaa ttcagccggc
     4741 tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg atcgaagtta
     4801 ccgtcgcttt tcgcaacagc aataccaaca cgtaaggcca gttgagcggc ctcaggctga
     4861 tctttcagcg ccaggatgta tttcatggtc tcgcccttgc cgatttcaac atcgaaatcg
     4921 aagcttgaaa ccagtttatt gaagaactcg atcacctcat tggtatcgaa gaccttcagc
     4981 tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact cacgccgtca
     5041 ctcgatacgg caatacgggc acatacagca acggtgccct gcatgaattt tttgtttttg
     5101 aaacgaccaa cctggcgggt cagttcgtca cgaccagagt taatggcacc tttaactttg
     5161 tcgaaaaagc tcataccagt ttcctttatt gctgtgttat ttagaaccgg cgctccagcg
     5221 aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact ggttgatacg
     5281 ctcaacttta atcgcaccgt tttcgtttac gagtctggca atggcgcaca atgtgcgacg
     5341 gttttcacct tcagtcaggc gggtctcaat gggcggttga tccggcacat aaatggtgac
     5401 cactccatca gtcttgtccc aactgggaac accttcataa ataaaggcgt aaatcagcac
     5461 ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt ctgatacatc
     5521 acccgtccgg tcatcgccct tgagctgaac ataaggttca tcgcgataat caccgaatgc
     5581 attaccgagc gcctgaatga ccgacttata cccgtcctga agctcaacaa aggctcccag
     5641 atccaggtcg atgcctttgg agccgaacat gcctgcaaag ccggatttgc cgctgccccg
     5701 gtgccagttc aggttgatcc tgatttcacc gaagttatcc cgtttggtca gactgattgc
     5761 aggcatctct tttgtcagag atactttact caggctgatt tttgtctcag ctggcagagg
     5821 gggtacaacc ggaggagtcg gggctgcggg agccggttcg tcggcaatat ttacgccgaa
     5881 atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc ggaatttcca
     5941 gccattatta cgacggtaaa attcacccag aattaaggct gcttcctgac ggccgctcag
     6001 ttcgacacag ccgctcacca ggccggtagc cccttgctcc acgtcgatgg aaagattacg
     6061 aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt tctggacatc
     6121 aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tgaccttcac tgaccaggct
     6181 gacagtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat ccccctgcac
     6241 cttcccggcg gcatacaggc gaaacgctga agcatccacc tggacgcccg aggttatccg
     6301 gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcatcaggt tcattagcga
     6361 ataaccgcag aaacgatatc ggagtgcata tcgtcaattg tgcgaccgcg acaggcatga
     6421 ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat aacgatcccg
     6481 gtatgtgagc cctgctcagt cagcttgtag caagccagct ctttgccggt ttgatcgacc
     6541 acacgacaga acgcattttc gacgtcattg aacgactgcc cacggaagct gttaaccgta
     6601 aacgccaggt attcaacgtt tgcaggcaga cgggagaggt tgaccttaat cacttcatcg
     6661 tcgccgtcac cctcaccagt caggttatca ccgctatgca caacagcttt gcaggtggat
     6721 tccagtttgc ggaaccagat ggtgtcaatt gttttaccgg tgctgtccat taatacacag
     6781 ccagcatcga gatcgattga gtcgttacct ccaaacaatc ctccaagaaa accttttttc
     6841 ttaaccggat cccaaccgag accgaaatga agctggctta atgcaggtga ttctttgctg
     6901 agtgataccg tctgattctt aactaatgaa accatgtttt attccttaaa atgctgataa
     6961 ctgagacaat caaaagtata tttagtaact ggcgaagaaa atcatatcat gatgaagttc
     7021 tctgacaagg gaaatttggt agctttctgt aaaaacacaa agagaaatcc ttttcatgat
     7081 gtcctttcat ctaaatacca attaaatatc aacacattaa caggtaacac aaaaacactg
     7141 atacactcca taacctcagg tgttattaat catattatga ttgacatgta gcatatacac
     7201 ccataaactc acttctcgac aataaatttt cttgccgttc cggataataa aaaaatgaac
     7261 aaaaaaattt ggtttatgga aggtttgtcc tctcagagag atattattca gggggttaaa
     7321 ttatttgcac aaaaaaataa ttttgccatt accgtttttg cctcccaccg taacgaaaga
     7381 aatgaaatcc tttccgttgc cgattattct ttgactgaac ctgaagatcc tcaaaaacgt
     7441 cttcagttta ttcaggaaac cattcagacc tacggcatcc accatattca tactggtcgt
     7501 aacaaccagt ggtttgaaga acaccgaacc gccattgaat cgaccggtgc cacccttact
     7561 accggtgcaa cgggcatcga ctggttaact ctggctgacg aaaaagttac ttttgctcag
     7621 tttatggagc aaaaggggct cccggtcgta ccatcctggc gggtgaatac gctggcagaa
     7681 ttaaagacac gcctcgcggc tccgccgttc actgacagtc cggtatgcgt gaagccggtg
     7741 acgggtatct acggcatggg attctggcgc tttgatgaca gtgctttgcc tatggccgtc
     7801 tttaatcacc ccgaacatcg tctggtcagc ccgcaactgt atattgctgc agcgtcggct
     7861 tctgagtcgt ttaaacccct tgttttgatg ccgtacctgc ctggcccgga attttccgtc
     7921 gatatcctcg cggataaggg cgaaatactc gcagccgtgg gacgccgtaa ggaaggggct
     7981 atccagtatc tggtaaacca aggaagcgcc tgggaactgg cgtgtgactg cgcccgggtt
     8041 atgaaggccg acgggctggt gaatgttcag acgcgaaacg atgtgaatgg caacccggtg
     8101 ctgcttgaaa ccaacatgcg tccgtcaggc ggggtgggtt atacccttca cagcggggtt
     8161 aaccttcccg ggttgtttgc tgcttttaag ctcggtctga tgtctgaaga tgcggtgatc
     8221 cagagcgcca gaaatacctt ttctccggtt gcagtgagat ccatcacaga tgtcatcgtt
     8281 tacccggaat cactctctaa ccttctgaat taaggcgtgt ttatgtctga taccctcgaa
     8341 gatatgattt atcgccgtac cctctcatgc ggtacgattc aggtaacccg cgaccagggt
     8401 gaagtttcgc tcgatgacct gtttgatatc gctgaaagac gtaacccgaa acgcgccttt
     8461 ctgtttgtca gcaaagtact cggcaggcac attcccgtgc caccctcagt catgcggcag
     8521 gcctacagac agcttgccag ccagttcccc tcaacgctga cagggcccat actgtttatc
     8581 ggtatggcag aaaccgccgt tgggcttggg gctggtgtgt ttgatgaagt gcgccaccag
     8641 catcgtgaat ctgtctatct gacctctacc cgtcacccgg ttgatggcac attgctttgc
     8701 gagttcaagg aagaacacag ccacgccacc gatcatctga tctatctgcc agacgatgaa
     8761 gagaaaagac gtcgtgttac aaacgcgcga actctggttt tgattgatga cgaagcaacc
     8821 accggtaata cctttattaa cctgctttct gccctgcgta ataccggcaa gcttcaacat
     8881 attgaacagg ttatcgctgt tacgcttacg gactggagcg aaaatgccct gtccaggcgc
     8941 agcactttac ccgtcacttc ggtttctctt gtaagtggtc agtggggatg gactccatta
     9001 ccagatgcac ctgtcccgga catgccgaag gtcaacgtaa cttcacgtgg aaaatgggac
     9061 atccagggaa aacagtcctg gggtcgactg gggatgctcg cacctgcggc cgatctcggt
     9121 catgaggtat ccttccgcaa gggggagcgt gttctggttc tcgggacagg ggaattcgtc
     9181 tgggagccgt tcctgcttgc tgaacggctc gaagctgccg gagcacaggc attttatggt
     9241 gcgaccaccc gctcccctat cgccgttggt tatgccatta agtccgcaat ttcctttacg
     9301 gataactacg gcctgggcat ccccaatttt gtctataacg tcgcccatca gcaatttgac
     9361 cgcattcttg tgtgtactga gacacctgca gaaagtattg acacgcggct tcttaaagcg
     9421 ctggctgagg ttgcacccgt cgtggagatt gttacctatg aataaacccg ttgttctgag
     9481 cgaccttgac gacacgctct ttcagacccg tcgtaaaatg gtagacgagc tggccctgga
     9541 gccattccgt accggtgccg ttgatcgtac tctgaatcca cgaagcttta tgacagaaga
     9601 acagtccatg ttagtggact ggctcctaga gcaggctgaa ctcattcccg ttaccgcacg
     9661 gggaactgaa gaaatcagcc gcgtaaggat ccctttccac tcctgggcaa tcaccacgca
     9721 cggggccgtc attctcacgc ctgaaggcaa acccgacgag gagtggaagg cccatatgct
     9781 cggccagcta actccctacc aggaaaagct ggcatcgatg cagcgtctga tcactgaaat
     9841 gatggacgca aaagggatca atgcctgggc aagactgaac ttcgaatacg gtgaaacggc
     9901 ggtttatatg gtgatgaaac accgcgacag cactcgcctt gacgaactca atgccattgc
     9961 agatgagata gaaacggtat tttcgaccga gggcttctac atccaccgca acagtaacaa
    10021 cgtagcctgg cttcccaccc cggttgagaa agggctggcc gtcagctggc tgcttgaaaa
    10081 acttcgggct gaacgcggag ttttccccgt aattggtctg ggcgacagcc tgagcgatca
    10141 tcgttttatg aaactgtgca gctggtttgg catcccgcgt cagagtcagt ttgcagatgc
    10201 catttcacag cgaatttttg gagaaaatta aaatgcagcc acatgaaaca tttaccggct
    10261 cataccagcc cggtgacgtg gaatttctgc taaagccggt tgtcattgaa atgacgccag
    10321 ttgatcaaaa agaagagctg attcagtccg ggaagaaaca ttattcggac atgctcagcc
    10381 aggagccagc gccgacacaa tggcatcttg atttgtttca ccgggcgctg gatcggggcg
    10441 cagagagact tgcaaaagaa gtcacacagc tcgctattgc tctggccgaa cgcttcggtg
    10501 atgagcccat agtactggcc agtcttgtca gggctggcgt accgctcggc gttatgctac
    10561 accaggctct gcgtgacatg ggaaaaacct catggcatta cggcatcagc atcatccggg
    10621 atcgtggaat tgacggtaca gcacttgata tcattgaaga gcggcatggt accagcggca
    10681 ttgtctttgt tgacggatgg acaggtaaag gcgcaattac cggagagctt gtacgcgcac
    10741 tgagagatcg cccgggctat cctgagcagc cgcgactggt tgtcctggcc gatccctgtg
    10801 gctgttcctg gcttgcagca agtgatgatg actggctcat tccttttggc atcatggggg
    10861 ctccggtgtc aggcctgctc tcccggtcag tatggtcctc tgaaggatta catgggtgca
    10921 tggtttgcga gcatctcagt gaattcgaat gcagccggat gcttgtcgat accgtcgctc
    10981 atttccgtaa gaagttaaca ccgtcatccc tcgctcctct gagctggaat acggagtcaa
    11041 cccgggtctt atggcagaca agtcgcgacg ttatcgcgtt cctggccgat gaatttaaag
    11101 tggacagcgt caaccgtatt aaacccggta ttgctgaagc aacccgggct gtattacgtc
    11161 gggtaccgga ccatgtattt gtgcgttcga ttgacgaccc ggacgttgcc ttacttgtgg
    11221 ggcttgctcg tgaaaaggga ataattgtta cagaaatggg gggaaccctc ggccagtatc
    11281 gggctgtaac cattatcaaa aaggtattct aatgaaaaat cgtctttctc cctggaatct
    11341 tggtgccact ctatacatgc ctgcaacacg ggaagatatt gctgatgccg ttctgcacga
    11401 gaaaatcccg ggtttgcgct ctctggtgat ttgcctggag gatgctgtca gtgaagcaga
    11461 catccccgtc gccctcaaaa atctagagca cctgctgcac gaactcagta acagcatgcg
    11521 tagcctgggt aaaaataact ggcccctgtt gttcattcgc ccccggcatg ccgaaatggg
    11581 caaatggtta aaagaacatt atgatctttc cgctgtcgac gggtttgtgt taccgaaatt
    11641 taccctaagt tcgttagctg aatggtggga tatcatgggc ggaactcacc tgagcatgat
    11701 gccaacgctg gaaacagaaa acgtctttga tgtgattcag atgcgcgagc tggccacccg
    11761 gctgttggaa catccctgcc acaagcgcat tattgcgctt cgaatcggcg gcaacgatct
    11821 tatgaacgtc gtgtcgcttc gccgtccccg agccctgacg ctgtatgaca gtccgatggg
    11881 ctacgtcatc aaaatgctcg tttcagtctt cagcccgcgt gattttgccc tgaccgcgcc
    11941 cgtatgtgag catattgacg atcatgccgt tatggccaga gagctggctc ttgatatggc
    12001 acatggactt gttggaaaaa cggccattca tccagatcag atagaggtca ttcaaaacgc
    12061 gctgatggtc actcagggtg agtactctga cgctctgcgg atcctgaact ctacccaggc
    12121 cgtgtttaag tcgcaggggg cgatgtgtga acctgccaca catcgccgct gggcggctgg
    12181 cattctggac agagcccggt tttacgggtt acataacgag caaagcgctg atggaatcag
    12241 attacttact ataacccagc atcattaagg aaatcataat ggggttcagg ttcagaggta
    12301 cttccgtttc tttctgtagc cggggggcct atgccaatct ggggttaccc ggtaccgagc
    12361 tgagttaccg tacccggttt gagctggccg cgccttccgg aggtggaaac aggacagcaa
    12421 ccgtcccgga tctcagacag tcgcttgagc aggaagcagt tgaactcatg tcagcggtga
    12481 cctcaattcg taacattcac gagttgacgc tggatccaaa aacaggcatc aattgggcag
    12541 agctggaagc agtatacctg catagcagaa catcgctttt tcaggttccg gcaccagtgc
    12601 gtcctgaaaa accagaatac catgcattgt cggaaaatcc tgcagggaac aaaggcattg
    12661 tttttctggg taaatggttt gaatcggaat cagctaaagc tgagcgtcaa gcagggaatc
    12721 ttcgccgatg gcggcaagaa ctgattgatg tgaagcgtga gaatcccctt cgacagcacc
    12781 ggtaccagca acggcagacg accagggccg aacagtattc aaactggaag tgtgatgctg
    12841 aagaacatga aaaaaggctc gccacgactc aggcagatgc ccggcagttg ttccgggcag
    12901 acgcagcgtt tttcgaatca taccttgcgg gtgtgctttc agaaactgaa tggccgcgtg
    12961 aaacgcttgt tgcatttgaa gtaaagccgg agatatcaac agtcctgccg gatgttggtt
    13021 tagctgagat tgaagatttc cctgataaga tttacggcgt caatgcccgg ggcatggagc
    13081 tgacgcaaaa agccatacgc gaaaactatg cccgccacag ccatggctgc ttgttccgac
    13141 tggtcggtat cgtgttacat acgctacctt tcgacaaggt gatcgcatca ggatttacac
    13201 aacgagtaag cggaacgact atctggagga tgtttatatc ctgtcctgca aatgcactcg
    13261 cagccagaag gcatagaaca tattgatccg gttgaagcgt taggctatca ccctgttatt
    13321 cgaaagatga gaaacacatt catttttcag cctattgaac cactaaccct ttaagcaaac
    13381 taaatattac gatgcaatta aataccagac aggccagaat ttttaagctt gccaacttac
    13441 tgggctccgg caagcctgtt tccgctaccg acattataac ctatctggac tgctcagagc
    13501 cgacattaac acgggccctc aaagagctac gaaagtcata ttctgcagaa atcaaataca
    13561 gtaaggcagg tcattcctat catctcgtta atccgggtca actggataaa aagacacttc
    13621 gccggatgaa tgaggcgctt gcacagaatg ctgatctgaa aaccggtgag tctacaggaa
    13681 aggtcgtact cgataaggat aaaaaaacag ccgtatcatt atcgctacgc atgcgaattt
    13741 taagaaaaat tgaccgtctg gctgcactga gtggatcgac ccgaagtgaa gctgttgaaa
    13801 aactggcact gcattccgtt gacgaactga taaaagaata cagcgctaaa aaatcctgat
    13861 gacaaatctc agctcggggt gaataccccg gttttttcat atttcaactc tgtcttggcg
    13921 tgacgtattt ttgatctatt ctcctcccgc aatgccagca gctccttaca gcctacaaaa
    13981 cctagcgtct tgacatcctc ttgtgctacg catcaatctt tgggcgattt acactccact
    14041 gactgccgta tgtagccatt aagccagtat tggtaacgta gatgcccacc ttttcactta
    14101 tttggtggac accactttgt ctaactcgtc agattctgac caaacgtttc aggctgtacg
    14161 cttactctga aggggggaga atagtagccc gtgatagcat tgagctaact gacagccaga
    14221 tcgcagcact aaagcgtaaa gccagtgatg acgaggcctg tggcgaaa
//
LOCUS       DAZGCQ010000119        14189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000119 DAZGCQ010000000
VERSION     DAZGCQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.119"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..386)
                     /gene="yafO"
                     /locus_tag="ACHK07_004122"
     CDS             complement(<1..386)
                     /gene="yafO"
                     /locus_tag="ACHK07_004122"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO8104390.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
     gene            complement(389..682)
                     /gene="yafN"
                     /locus_tag="ACHK07_004123"
     CDS             complement(389..682)
                     /gene="yafN"
                     /locus_tag="ACHK07_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO8104391.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(734..1789)
                     /gene="dinB"
                     /locus_tag="ACHK07_004124"
     CDS             complement(734..1789)
                     /gene="dinB"
                     /locus_tag="ACHK07_004124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO8104392.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1860..2645)
                     /gene="lafU"
                     /locus_tag="ACHK07_004125"
     CDS             complement(1860..2645)
                     /gene="lafU"
                     /locus_tag="ACHK07_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO8104393.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2617..4329
                     /locus_tag="ACHK07_004126"
                     /pseudo
     CDS             2617..4329
                     /locus_tag="ACHK07_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4522..5019)
                     /gene="rayT"
                     /locus_tag="ACHK07_004127"
     CDS             complement(4522..5019)
                     /gene="rayT"
                     /locus_tag="ACHK07_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO8104394.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGNITDFSAG
                     ERIIS"
     gene            complement(5195..5944)
                     /gene="yafL"
                     /locus_tag="ACHK07_004128"
     CDS             complement(5195..5944)
                     /gene="yafL"
                     /locus_tag="ACHK07_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8104395.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6154..6414
                     /gene="dinJ"
                     /locus_tag="ACHK07_004129"
     CDS             6154..6414
                     /gene="dinJ"
                     /locus_tag="ACHK07_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO8104396.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDLFDKLGI"
     gene            6417..6695
                     /gene="yafQ"
                     /locus_tag="ACHK07_004130"
     CDS             6417..6695
                     /gene="yafQ"
                     /locus_tag="ACHK07_004130"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO8104397.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            6851..7591
                     /gene="dpaA"
                     /locus_tag="ACHK07_004131"
     CDS             6851..7591
                     /gene="dpaA"
                     /locus_tag="ACHK07_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO8104398.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7562..8329)
                     /gene="yafJ"
                     /locus_tag="ACHK07_004132"
     CDS             complement(7562..8329)
                     /gene="yafJ"
                     /locus_tag="ACHK07_004132"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO8104399.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETSNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8535..9113)
                     /gene="lpcA"
                     /locus_tag="ACHK07_004133"
     CDS             complement(8535..9113)
                     /gene="lpcA"
                     /locus_tag="ACHK07_004133"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO8104400.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9353..11797
                     /gene="fadE"
                     /locus_tag="ACHK07_004134"
     CDS             9353..11797
                     /gene="fadE"
                     /locus_tag="ACHK07_004134"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO8104401.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAHSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKDLGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11840..12286)
                     /gene="ivy"
                     /locus_tag="ACHK07_004135"
     CDS             complement(11840..12286)
                     /gene="ivy"
                     /locus_tag="ACHK07_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO8104402.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12467..13237
                     /gene="yafV"
                     /locus_tag="ACHK07_004136"
     CDS             12467..13237
                     /gene="yafV"
                     /locus_tag="ACHK07_004136"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO8104403.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(13295..13663)
                     /locus_tag="ACHK07_004137"
                     /pseudo
     CDS             complement(13295..13663)
                     /locus_tag="ACHK07_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(13709..14189)
                     /locus_tag="ACHK07_004138"
                     /pseudo
     CDS             complement(13709..14189)
                     /locus_tag="ACHK07_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT         3383 a         3642 c         3661 g         3503 t
ORIGIN      
        1 cgaaacgctt ctgccatttt cccaatttta tgcatttggt tgttatctcg agccagttta
       61 tgaggttcag gtttcagaat ggcaatgagc aaccatgctt gctcatcaaa cgcaccctga
      121 caatatacca aatgcgcttc gtcattcgtt ctgctgaatt ggcgcaactg tggcggaaat
      181 ggattattca catttgccag atgaatatga gcaactcgct caaatttgat taatggccag
      241 gtaaacgagt cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac accgtcacgc
      301 ttataggaaa taaaatccgc cgttaacgca tcaagttcct ctgctgtaag ttgcaggcga
      361 ataagttttg ttttgaatac ccgcatcctt attccttaaa gtcattgaaa tcatcatccg
      421 tcatatcatt aagatattgt tcagcgcggc gtgtaatttc ctctaatctt gcagcacttg
      481 gacggaagcg cgcctttatg ggctttttct cctcttgttc agcaagcatg tccattaacg
      541 cttcgaatgc gctggcactt aagagatatc ctgcggggcg attattagaa agaaccgcaa
      601 ccggttgatc aataaagtat ttagctgggt ttttacgtaa ctcagtgata ttgaccgatt
      661 tttcagcgag aattcgatgc atacagtgat cccctcataa taatgcacac cagaatagac
      721 ataatagtat acatcataat cccagcacca gttgtctttc catttgaggg tcaagcaacg
      781 tcacatgcag ccccaccagt cgcacaccgc gcccaccgcg gcgttcatcc caggttttac
      841 gcgcggtggc gattagatca gctttattca gccgcggcca gacgtgctcc tgggtggttt
      901 gctgaaaatc gtcgaacttt aatttcaccc cctggcgagc aatcagtaaa tcaggtttta
      961 cctttgccag acggcgttca agttccggat acagccgctc gataatcgct tcacattcag
     1021 accagtgatg aatatcttcc gccatcgtgc gttccacgcc gacggatttt cgcaaccgtt
     1081 cgctgttaac atcgcgttcg tcaatcccct gactacgctc ccacaaaatg cggccaaatt
     1141 tgccaaagcg tttaagcagc atcaccagat cacacttttg cacatcaccg caggtccgca
     1201 gccccatcgc ttccagtttt gccgccgaga ctttgccgac gccggggatt tttgccagcg
     1261 gtaaggtttg cagaaatgcc ggaacttctg ccggagtaat cacaaactgg ccgttgggtt
     1321 tattcatgtc ggaggcgatt ttggcgagaa actttaccgg cgcaacgccc gcagacgccg
     1381 tcagttgcag ctcgttgaaa atcgtctggc ggatttcctg ggcaatgagg gtcgcagaac
     1441 cgtggcaatg gacgctgtcg gtgacatcga gataagcctc atccagtgac aacggctcaa
     1501 tgcgagaggt gtagcgcgag aagatttcac ggatatgatt tgaggcttct ttgtaggcgt
     1561 caaagcgccc cggaagcaag gtgagatgtg ggcataattt gagcgccatc cctgtcggca
     1621 tagcgctgcg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc accccccgac
     1681 gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcgggattg tcgcgcatct
     1741 ccactgcggc gaaaaagcag tccatatcca catgaatgat tttacgcatt actcacctct
     1801 caacactggt aaagtataca gtgatttcag ggtttgagaa atgcgtaaag attcagcatt
     1861 taacgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag cggctgcacc
     1921 actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact tttggtcagc
     1981 accataatct caatacgccg attgcccgcg ctttgcggat ttttggcatc cagcagcatc
     2041 tggtccgcca ttgcgcttac ctgcatcact ttatcttccg gcattccggc ctcttccagc
     2101 acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc agttgttgta gatattgttt
     2161 ttgtaggcca tcgcatcggt atgcccggta ataataattt tgttatcgag cgagtcgaac
     2221 actggcgcga gctccaccag caaagtttta aagaacggca taatctgcgc gctgccgcgt
     2281 tcgaacatat tgcgattctg gtcgtcttta atcagcacgc gtaatccctg cgggacaatc
     2341 tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtgttgat actggttgcc
     2401 aactcgccaa gctcggtggc ggatttcttc ttcagcagcg ccgttttctc attcacatca
     2461 cgcgcttttt tctccgtctc ttccggcacc gccacggtgg caggttttgg cggatgagac
     2521 gggctgattt tatttaacgg cgacagccca ccaccgttaa aaatcgactg cccgtgcagc
     2581 gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcaa ctttatcgtc
     2641 gtcaccaaag gggccgagcg tatttccgag gtttctgccc gctttaccct ggacgcgatg
     2701 cccggcaaac agatggcgat tgacgccgac cttaacgccg ggttgatcaa ccaggcgcag
     2761 gcgcaaaccc ggcgtaaaga tgtcgccagc gaggccgatt tctacggcgc gatggacggg
     2821 gcatcaaagt ttgtgcgcgg ggacgccatc gccgggatga tgattctggc gatcaacctg
     2881 atcggcggtg tctgtatcgg gatcttcaaa tacaacctga gcgccgatgc tgccttccag
     2941 caatatgtgc tgatgaccat cggcgacggg ctggtggcgc agatcccttc cctgctgctc
     3001 tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca acggcgatat cgcccatgac
     3061 gtgcgccacc agctgctggc aagcccgtcg gtgctctaca ccgccaccgg gattatgttt
     3121 gtgctggcgg tggtgccggg aatgccgcat ctgccgtttt tgctgttcag cgccctgctt
     3181 ggctttaccg gctggcggat gagcaaacgc ccgcaggcag cggaggcgga ggagaaaagt
     3241 ctcgaaacgc tgacccgcac catcaccgaa accagcgagc aacaggtcag ttgggaaacg
     3301 ataccgctga tcgagcctat cagtttaagc ctcggttaca agctggtggc gctggtggac
     3361 aaagcccagg gcaacccgct cacccagcgt attcgcggcg tgcgacaggt gatatccgac
     3421 ggcaacggcg tgctgctgcc ggagatccgc attcgggaaa acttccgcct taagcccagc
     3481 cagtacgcta ttttcatcaa cggcattaag gctgatgaag cggatattcc ggcggataaa
     3541 ctgatggccc tgccctccag cgaaacctac ggcgagattg acggcgtgct ggggaacgac
     3601 ccggcgtacg ggatgccggt gacctggatc cagcctgcgc agaaagcgaa ggcgctgaat
     3661 atggggtatc aggtgatcga cagcgccagc gtgattgcca cccatgtgaa taagattgta
     3721 cgcagctata ttcctgattt gtttaactat gacgacatca cacagttgca taaccgtttg
     3781 tcgtcgatgg caccgcgcct ggcagaagac ttaagcgcgg cgctgaatta cagccagttg
     3841 ctgaaagtgt accgcacact actgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc
     3901 gccaccgtgc tggtcgccag tagcgcggtg actaaagatc atattctgct ggcggccgat
     3961 gtacgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaagca ggagctgacg
     4021 gtgtatacgc tgaataatga gctggaaaat ctgctcacca atgtggtgaa tcaggcgcaa
     4081 cagggcggca aagtgatgct cgacagcgtg ccggttgacc cgaatatgct caaccagttc
     4141 cagagtacga tgccgcaggt aaaagagcag atgaaagcgg cggggaaaga cccggtgctg
     4201 ctggtgccgc cgcagttgcg tcctttgctg gcgcgctatg cgagattgtt tgcgccgggg
     4261 ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat tatgggggcg
     4321 ttgatgtagg tatccggcat cggtgcacaa ccgccggatg cggcgtgaac gccttatccg
     4381 gcctaccgat ccggcacagg tttgtaggca tgataagacg cgccagcgtc gcatcaggca
     4441 tccgcgcaca attgccggat gcggcacaag tttgtaggca tgataagacg cgctagcgtc
     4501 gcatcaggca tccgcgcaca attatgaaat gatacgctcc ccggcactaa aatccgttat
     4561 gttacccgcc caatcaatgg gatataaccc tcgcgccaca tcgcgatgga acgttgagaa
     4621 tggccaatca ctcacttgct ttacccagcc atgttttact ggatttatat aaatataatc
     4681 aacatgatgg cgatagtctt tggtattgcg gatggtgtgc tcccaaaaac gtggttgcca
     4741 gatatttttc aatccacaag catgggtgaa ttgcttttta atctcccgcc agcgagagga
     4801 aaaatcatca tcgctttcag gtaatgtcca gatacagtgc atatgctccg gcaaaactac
     4861 ccaggcatta atttcaaaag gtctggctcg cttaacttta ataatggcgt tacggagcgt
     4921 ctggtactgg gtagtcaaaa gttggcttcg acgattccgt aaattcaccg tgaaaaacca
     4981 gcttcccccc ttgaggtaat aacgacgata ttcagacata gcgactcctt tttctcaacg
     5041 gtaagaagaa aaggaaaggt tggcaatgag gttgttttat atcgggaaag cggctcgcaa
     5101 atattcgcgg gctcgtaggc atgataagac gcgccagcgt cgcatcaggc atcggtgcac
     5161 aactgccgga tgcggcgtaa acgccttatc cgtcctacaa aatcgtctct tccgtcaaaa
     5221 tccggcgcgc gcctaaaaaa tgatcctgcc agaaaggttc agctaaccga cttacccgaa
     5281 tggtttcccc ggtgcgcggt gactcaataa actgaccgtc gcccagatac acccccatat
     5341 ggtcggcaat ctcgcggctg tggatatgga aaaacagcaa atctccccgg cgcaggtcgt
     5401 tattcgccac aatcgttgcc cggcggtagt gatacatctc attggccgtg cgcgggagct
     5461 tcgcctcgag gatcttgttg taggcataaa aaaccaaccc gctacagtcg aagcctttat
     5521 cgggccgcgt gccgccccag acgtaaggct tgccaagctg ctgctccagg cggtgaatcg
     5581 ccacttcggt gatgttatgc aggtgatcgc tgcttaaaaa gtggttgttt tccgccagcg
     5641 cctgccagcg tctgtcactc gccttgagcg gcgcaggaaa ccattctgga tgctgtttta
     5701 tctgttcgcg gttgtgctgg cgcagcgccc ttctgctttc ggcattgcct tccacaatat
     5761 agctggcctg ctttttcaga tgggtttggt actgttttaa taaacgcgca cggttttgca
     5821 tccgctggcg ggcggcgtag ctgaaagaga tatgtgatgt ggtggcgctg gcaaatgaag
     5881 aaaacggcaa acaaatcaac agtaatcccg acaatacaaa ggatggaatc gacggcaagg
     5941 acatactcaa taatggctgg tcatgcttta atgttgcagt gtatcccgga tcgtttctga
     6001 acaaacttaa taaaccatta ttataaaatt aacatatata tttcagtgaa ttatgtaaat
     6061 acatcagttt attgaattgc tcataaattg ccgctgttgc tcaattgagc tacaattcaa
     6121 gctgaatata tatacagcac aggagatacc ccaatggctg caaatgcatt tgttcgcgcc
     6181 cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac tggccgggat ggggctgacc
     6241 atctctgacc tggttcgcat aaccctcaca aaggtcgcgc gtgaaaaggc attgccgttt
     6301 gatttacgcg agcctaatca attaaccatt caatcaatca aaaacagcga agctggcgtt
     6361 ggtgttcata aggccaaaga cgccgatgat ttatttgata aattaggaat ttaaatatga
     6421 ttcaaaggga tattgaatac tcgggacaat tttcaaagga tgtaaaactt gcacaaaagc
     6481 gtcataagga tatgaataaa ttgaaatatc ttatgacgct tcttatcaat aatactttac
     6541 cgcttccagc tgtttataaa gaccacccgc tgcaaggttc atggaaaggt tatcgcgatg
     6601 ctcatgtcga accggactgg ctcctgattt acaaacttac cgataaactt ttacgatttg
     6661 agagaactgg aactcacgcg gcgctctttg ggtaaagccc gctgccgatg aaatttgtaa
     6721 accctgcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc
     6781 agactattcc gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg
     6841 aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc
     6901 gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca
     6961 gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct
     7021 ctacgtcaaa atgggtgagc aatatcaact gctcgacagc tataaaatct gtaaatattc
     7081 cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag
     7141 cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta atattggttt
     7201 ccccaatgcc tatgaccgtg cgcatggtta tgaagggaaa tacctgatga tccacggcga
     7261 ttgtgtttcc atcggctgct acgcaatgac caatcagggt attgatgaga tcttccagtt
     7321 cgttactggt gcgctggtgt ttggtcagcc gagcgtgcaa gtgagtattt acccgttccg
     7381 catgaccgac gctaatatga agcgccacaa atattccaac tttaaggact tctgggagca
     7441 actgaagcca ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt
     7501 caatggccgt tacgtggtca gcaagccgtt aagccacgaa gttgtgcaac cacaactggc
     7561 atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata atcttttgcc
     7621 aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg
     7681 tctgcgagct gaagtcgatt tctacatcct gatccagcaa cgttgccacg ccaaacggcg
     7741 cgcggcgggt gatccagtgt aaattagtcg agcaatacgc cattacatag cgaccgtccg
     7801 aaagcagcat gttgaaaacg cccttcgccc gcagttcatc cgccagtgag gcgatatatt
     7861 taaacactgc cgccatgttg cccggcgtgc gcgggtaacg ctgcgttaat ttatgcagga
     7921 gccagcaaaa ggctttttcg ctgtcggttt caccgaccgg gcggaagttg ctggtttcca
     7981 gtgatttgta gcccgtcagt tgcccgttat gggcataagt ccagttacgc ccccataact
     8041 cgcgggtaaa cgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat
     8101 gagccaccac cgaacaggat tttatcgggt agtcctggac cagtttggcg ataggtgagt
     8161 taaagctggg ttgcggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc
     8221 cccagccatc tttatgtggc ccggttccac caccacgctg caccagaccg gtgaaactaa
     8281 agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc
     8341 acaacagcgg gagcgcatgg cccccggcga agattgattt aagaattttc aggtcggatg
     8401 cggcgtgaac gcattattcg gcctacggtt cgcgtacaga tttgtaggca tgataagacg
     8461 cgtcagcgtc gcatcaggca tcggtgcaca attgccggat gcggcgtaaa cgtcttatcc
     8521 ggcctacgcc agacttactt aaccatctct ttttcaatca actggatcag gatatggatc
     8581 actttaatgt gaatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg
     8641 atatccgccg tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatt
     8701 cccttctcac gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag
     8761 atccccagca gtacatcgcc ttcgcgacct accgcttcaa cgtaacggga gaaaatatca
     8821 ttgaaaccga aatcgttacc gacgcaggaa atatgactaa cgtcagaaat agcaatcgcc
     8881 gggtagcccg gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg
     8941 tcgcaatggg aaccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct
     9001 gctaacagga ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta
     9061 gccagcgttt ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata
     9121 tccttcagca taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa
     9181 gtacgggcag gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca
     9241 atgtgagctt tgttgtaatt atattgtaaa cgcattgcta aatgttttta catccactac
     9301 aaccatatca tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat
     9361 tttgagtatt ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt
     9421 atttatcagc agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg
     9481 gtcggcgtgg gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat
     9541 gcgtaagtcg atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat
     9601 gtcgcgcact gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt
     9661 ccagggcaag ccggactgga aaaagctgca taactatccg cagccgcgcc tgacggctga
     9721 agagcaagcg tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca
     9781 gatcacccat gaactggcgg atctgccgcc agagttgtgg gcgtacctta aagagcatcg
     9841 cttcttcgcg atgatcatca aaaaagagta cggcgggctg gagttctcgg cttatgccca
     9901 ctctcgtgtg ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt
     9961 gccgaactca ttaggcccgg gcgagctgct gcaacattac ggcactgacg agcagaaaga
    10021 tcactatctg ccgcgtctgg cgcgtggtca ggaaatccct tgttttgcgc taaccagccc
    10081 ggaagcgggt tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggtgaatg
    10141 gcagggccag caggtgctgg gcatgcgact gacctggaac aaacgctaca ttacgctggc
    10201 accgattgcg accgtgctgg gtctggcgtt taaactctcc gacccggaaa aattactcgg
    10261 cggtgcagaa gatttaggca ttacctgtgc gctgatcccg accaccacgc cgggcgtgga
    10321 aattggtcgt cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa
    10381 agatgtcttc gtaccgatcg attacattat cggcggacca aaaatggccg ggcaaggctg
    10441 gcggatgctg gtggagtgcc tctcggtagg ccgcggcatc acactgcctt ccaactcaac
    10501 cggcggcgtg aaatcggtag cgctggcaac cggcgcgtat gctcacattc gccgtcagtt
    10561 caaaatctct atcggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccgggaa
    10621 cgcttatgta atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa
    10681 acctgccgtg ctgtcggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat
    10741 tattgatgcg atggatatta ccggcggtaa aggcattatg ctcgggcaaa gcaacttcct
    10801 ggcgcgtgct taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac
    10861 ccgcagcatg atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga
    10921 gatggaagcg gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat
    10981 cggtcacgtc ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac
    11041 cagcagcacg ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag
    11101 cgccaacctc gccctgcttt ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg
    11161 tcgtgagcgt atctcggcac gtctgggtga tattttaagc cagctttacc tcgcctctgc
    11221 cgttctgaag cgttatgacg acgaaggccg taatgaagcc gatctgccgc tggtgcactg
    11281 gggcgtacag gatgcgctgt ataaggctga acaggcgatg gacgatttac tgcaaaactt
    11341 cccgaaccgc gtggttgccg ggctgctgaa tgtagtgatc ttcccgaccg gacgtcatta
    11401 tctggcaccg tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc
    11461 cactcgctca cgtattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg
    11521 tttactggaa gaggcgttgg tggatgtgat tgccgccgac ccgattcatc agcggatctg
    11581 taaagatctg ggtaaaaacc tgccatttac ccgtctggat gaactggcgc acaacgcgct
    11641 ggcgaagggg ctgattgata aagatgaagc cgctattctg gtgaaagcgg aagaaagccg
    11701 tctgcgcagt attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa
    11761 gttgccggag aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct
    11821 ccgtttcttt atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc
    11881 cggtcaacgc cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca
    11941 accaggtgag tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct
    12001 gattagattt ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc
    12061 acgcgctcat cacctgatac gtctcatctc ccaatgtcac ggtttgtgcg ggagtataag
    12121 taccgccttt catcacccat gcaggtagct tatgcccttg caccatctga ttaaacgcag
    12181 ctttggtggt ttcgcccttt gccaggctgc taatggttaa atcatcctgc gccattgcac
    12241 tggtggcgat gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc
    12301 ttatcctgcc catgttatta acctcctaaa gcctggcgat aacacgataa tttgcaaatc
    12361 agatatttcc aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat
    12421 aaggtgttaa agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa
    12481 gattacgctt ttgcagcaac cgttggtgtg gatggatggt cctgccaacc tgcgtcattt
    12541 tgatcgtcaa ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac
    12601 cagcggcttt gccatggaag cggcagcttc gtcgctagcg caagatgacg tagtgaactg
    12661 gatgacagct aaagcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac
    12721 ggagtctggt tcagttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta
    12781 tgataagcgt catctgttcc gaatggctga tgagcatctg cattataaag cgggcaatgc
    12841 gcgagtgatt gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg
    12901 ttttcctgtg tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg
    12961 gcctgctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca
    13021 ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg
    13081 tgacagccgg gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc
    13141 aacgcgcatt gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc
    13201 ggcatggcag gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttagggccgg
    13261 ggagagtcgg ggtatgacta cgaaagcccg ctctcctgcg accactttta tcataggagt
    13321 gtggaagtgc ttttccatca atcgcgatga cgtctttgtc atttgatgaa tggcagtcac
    13381 gcatccagtt aataaagcac tcgtgaaatt ttgcaggact gatacaggat acaactctgg
    13441 caatggtatc gtgaacagga ataccatttt caaaatcacc atattgcttc aaaaaatcga
    13501 gatgtgtttc cccaaaatcc tctatatctt cccaaccttc tgcaccagaa ataacggcac
    13561 aaatagtcaa cagtagaata tccgataatt tatgttccac gttccaggct tgtctgtaat
    13621 cggggataac agaaatatgt tccatcaatt ttttaagttc cattttgttc tccttaatta
    13681 tgtaaggagt atttgatcca gggcaagatt acgaaagccc gctccccgca aggactgacg
    13741 ccagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata
    13801 ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat ccctgaaaat aattctgctg
    13861 catttcctct tcttattctg cagtcgtctt cattcattac cacgtccagg cgccagtgca
    13921 gcttattttc cacgtgccag tgatttcggt tcgctgtggc gaacttctct gcagttaaat
    13981 cagcagagct gatataatat ctgaccgtca tttcgggttc tttcttttgt tctgctatta
    14041 ttgaccgaaa ggagactgcc acgcataatt tcttcagccc ttttcattca aacgtgaaat
    14101 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
    14161 tcttttcact gattgcgtaa ctgtcatgc
//
LOCUS       DAZGCQ010000120        14179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000120 DAZGCQ010000000
VERSION     DAZGCQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.120"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            233..5653
                     /locus_tag="ACHK07_004139"
     CDS             233..5653
                     /locus_tag="ACHK07_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO8104404.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATILEGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINSKDYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSDVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDIENTTSVNVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGVSDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDNHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGV
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDLTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMSGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSRLSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDSNKTGAYVDCWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHNRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5864..6130
                     /gene="hipB"
                     /locus_tag="ACHK07_004140"
     CDS             5864..6130
                     /gene="hipB"
                     /locus_tag="ACHK07_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO8104405.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6130..7452
                     /gene="hipA"
                     /locus_tag="ACHK07_004141"
     CDS             6130..7452
                     /gene="hipA"
                     /locus_tag="ACHK07_004141"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO8104406.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7723..7920
                     /locus_tag="ACHK07_004142"
                     /pseudo
     CDS             7723..7920
                     /locus_tag="ACHK07_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7925..8242
                     /locus_tag="ACHK07_004143"
                     /pseudo
     CDS             7925..8242
                     /locus_tag="ACHK07_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            8603..9313
                     /locus_tag="ACHK07_004144"
     CDS             8603..9313
                     /locus_tag="ACHK07_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8104407.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKDEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9355..12006
                     /locus_tag="ACHK07_004145"
     CDS             9355..12006
                     /locus_tag="ACHK07_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8104408.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSECTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTNGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDIYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLRLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            12020..12550
                     /gene="ydeS"
                     /locus_tag="ACHK07_004146"
     CDS             12020..12550
                     /gene="ydeS"
                     /locus_tag="ACHK07_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104409.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12563..13066
                     /gene="ydeR"
                     /locus_tag="ACHK07_004147"
     CDS             12563..13066
                     /gene="ydeR"
                     /locus_tag="ACHK07_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104410.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13126..14040
                     /gene="ydeQ"
                     /locus_tag="ACHK07_004148"
     CDS             13126..14040
                     /gene="ydeQ"
                     /locus_tag="ACHK07_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104411.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENISLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3967 a         3047 c         3547 g         3618 t
ORIGIN      
        1 gaaacagttt tcttattaag caacaataac tcatagttca tttttaaata aaataagaac
       61 tatgcaattt ttaatctatc aattcgaaag cattagcaaa ttagccaaat tgacaccttc
      121 gcacctccaa ctaacactca atacagacct ctgacaattt aataagtttc atttttaatg
      181 tctgacgact catctctttc gtggctgtat tgtttgtata tggagcgttt atatgaatag
      241 aatctatcgc gtgatatgga attgcactct acaggtattt caggcctgct cggaattaac
      301 tcgcagggca ggtaaaacat cgacggttaa tttgcgtaaa tcctctggac tgacaacgaa
      361 attcagtaga ttgacgctgg gtgttttgct ggcactaagc ggttcagcgt ctggtgcgag
      421 tctggaagtt gataatggtc agattaccaa tattgatact gatgttgctt atgatgccta
      481 cctggttggc tggtatggca ctggagtgct taatattttg gctggcggta atgcctcctt
      541 aaccactatt actaccagcg tcattggcgg taatgaggac tcagagggca ccgttaatgt
      601 tttgggtggc acctggcgat tgtatgatag cggaaataat gcaaggcctt taaatgtggg
      661 tcaatccgga acggggacgc tgaatattaa acagaaaggt cacgttgatg gaggctattt
      721 aagactaggt tcctcgacag gaggcgtcgg gacggtcaat gttgagggag aggactcggt
      781 tctgacgacc gaattattcg aaatagggag ttacggtacg ggctcattaa atattacgga
      841 taagggttac gtcacgagtt caatagtcgc cattttagga tatcaagcga acagtaatgg
      901 taaggttgtc gttgaaaagg gtggcgagtg gctaataaaa aataatgatt cctcaattga
      961 atttcaaatt ggtaatcaag gaactgggga ggcgactatt ctcgagggtg ggctgattac
     1021 ggctgaaaat acgattatcg gtggcaatgc caccggtgtc ggaaccctga atgtgcagga
     1081 tcaagactct gtcatcacgg tacgcagact ctataatgga tatttcggta atggcgcagt
     1141 caatatttcc aataatggac taattaatag caaagattat tcattggtgg gcgttcagga
     1201 cggttcccac ggtgtcgtca acgtgaccga taaagggcat tggaatttcc tcggaacagg
     1261 cgaagctttc cgctatatct atatcggtga tgctggcgac ggtgaactta atgtctcgag
     1321 tgaaggcaaa gtagattcgg gaattatcac tgcggggatg aaagaaacag gcacaggcaa
     1381 cattactgtt aaggataaga actccgttat cactaatctc ggaactaatc ttggttatga
     1441 cggccacggc gaaatgaata tcagtaatga ggggcttgtt gtcagcaacg gaggaagttc
     1501 actcggttat ggagaaaccg gcgtcgggaa tgtcagcatc accacggggg ggatgtggga
     1561 ggtcaataag aatgtctata ccaccattgg tgttgcgggt gtcggaaacc tcaatattag
     1621 cgatggcggt aagtttgtat cgcaaaatat tacttttttg ggcgataaag caagcggtat
     1681 cggcacactg aacctgatgg atgcgacatc atcgttcgat actgtgggta tcaatgtcgg
     1741 taattttggt agcggtatcg taaatgtcag taatggtgcc acccttaatt caacgggcta
     1801 tggatttatc ggaggaaatg cctccggtaa gggaatagtt aatatttcaa cggatagtct
     1861 ctggaattta aagacgtctt ctactaacgc ccaattgcta caggtcggtg tattaggcac
     1921 gggtgaactg aatattacca ccggaggtat agttaaagcg cgtgatacac agatagctct
     1981 caatgacaaa agtaagggcg acgtgagggt ggatgggcag aactctcttc ttgaaacatt
     2041 caatatgtac gtagggacat ctggtacggg tacgttaacc ctgacgaata gcggcacgct
     2101 gaatgtcgaa ggtggagaag tttacttagg tgtttttgaa cctgctgtag gaacgctaaa
     2161 cattggtgca gctcacggtg aggcggcggc agatgccggg tatataacca acgcgacaaa
     2221 agtagagttt ggctctggcg aaggtgtttt tgtctttaat cataccaata acagtgatgc
     2281 cggctaccag gtcgatatgc tgattacagg tgacgataaa gacggaaaag tgatccatga
     2341 tgcaggccat acggtgttca atgcagggaa tacttatagc ggtaaaacgc tggtcaatga
     2401 cggcctcctg accattgcgt ctcatacggc agatggggta acgggcatgg gttcgagtga
     2461 tgtaaccatt gcaagccccg gtacgctcga cattctcgca tcaacgaaca gtgcaggaga
     2521 ttacacgctg accaatgcgc tcaaaggcga tggcttgatg cgagttcagc tgtcatcctc
     2581 cgacaagatg tttggcttta cccatgcaac agggactgaa ttcgccggtg ttgcccaact
     2641 gaaagacagt accttcactc tggaacgcga caacaccgct gcgcttactc acgcgatgtt
     2701 gcagtctgac attgaaaata ccacatcggt aaacgtggga gagcaatcca ttggtggact
     2761 ggccatgaat ggcggtacgc tcattttcga tacggatatt cctgctgcga cgcttgcaga
     2821 gggatatatc agcgtcgata cgctggttgt cggcgtgagt gactacacct ggaaaggccg
     2881 taactatcag gtaaacggga cgggcgacgt gcttatcgac gtgcctaaac cgtggaatga
     2941 tcctatggcg aataaccctc tgacgacgct caatttgctg gaacacgatg ataaccatgt
     3001 cggcgttcaa ctggtgaagg cgcaaacggt tattgggtcg ggtggctcat taacgttacg
     3061 tgatttacag ggcgacgagg tggaagcgga caaaacgtta cacattgcgc aaaacggaac
     3121 ggtggtcgcc gagggtgatt atggagtccg cctcacgacc gcaccaggtg atggtttgta
     3181 cgttaactat gggctgaaag cgctgaacat ccatggaggg caaaagctga cgttagccga
     3241 acatggcgga gcctatggcg caacggccga tatgtcggca aaaatcggtg gtgaagggga
     3301 tctggcaatc aatacggtgc gacaggtttc gctttccaac ggtcagaacg actatcaggg
     3361 ggcaacctac gttcagatgg ggacattacg taccgatgcg gatggcgcgc tgggcaacac
     3421 ccgggaactg aacatcagca acgcagccat cgtcgatctt aatggatcga cgcagacggt
     3481 agagacattc accgggcaga tgggttcgac tgttttgttc aaagaagggt cgctgacggt
     3541 aaataaaggt gggatcagtc agggtgaact gacaggtggc ggaaacctga atgttacagg
     3601 gggaacgctg gctattgagg ggcttaatgc acgctacaat gcgttaacca gcgttagccc
     3661 aaatgcggaa gtcagcctcg ataatacgca ggggttaggc agaggaaata ttgccaatga
     3721 cggtctgtta acgctaaaaa acgtgactgg cgaactgcgt aatagcataa gcgggaaggg
     3781 tatcgtgagc gcaaccgcca ggacagatgt agagctggat ggcgataata gccgctttgt
     3841 ggggcaattc aacattgata caggcagcgc gctcagcgtc aacgagcaga aaaacctggg
     3901 tgatgcttcc gttatcaata atggcctgct caccatctcc actgagcgta gctgggcgat
     3961 gacgcacagt atcagcggta gcggtgattt gacaaaactg ggtaccggga tcctgactct
     4021 taacaacgat tccgcggcgt atcagggtac gacggatatc gtgggtggtg aaattgcttt
     4081 cggttccgac tctgccatta atatggcaag tcagcacatt aatatccata acagcggtgt
     4141 gatgtcggga aatgtcacca ctgcaggtga tgtgaacgtt atgtctgggg ggacactgcg
     4201 tgtcgctaaa accacaatcg gcggcaacct ggagaatggc ggcacggttc aaatgaacag
     4261 tgaaggagga aaaccgggga atgtactgac cgttaacggc aactataccg gaaacaatgg
     4321 cctgatgacg ttcaacgcga cgctgggcgg cgataattca cccaccgata agatgaacgt
     4381 gaaaggcgat acccaaggga acactcgcgt tcgggttgat aacattggcg gcgtcggtgc
     4441 gcaaacggtc aacggtattg aactcattga ggttggcggt aattctgcag gtaacttcgc
     4501 gttgaccacc ggaactgtcg aagctggggc ttacgtctac acgctggcta aagggaaggg
     4561 gaatgacgag aaaaactggt atctgaccag taaatgggac ggcgtaacgc cagcggatac
     4621 acccgatccc atcaataatc cccctgttgt ggatccggaa ggcccatcag tttatcgccc
     4681 ggaggccgga agctatatca gcaacattgc cgcagccaac tcgctgttta gccatcgctt
     4741 acacgaccgt ctgggtgagc cgcagtatac agattcacgg ctttctcagg gttcggcaag
     4801 cagtatgtgg atgcgtcatg tcgggggaca cgaacgtttc aggaccggtg acggccagct
     4861 aaatactcag gctaaccgct atgtattgca gctaggcggc gatttggcgc agtggagtag
     4921 caacgcgcag gatcgctggc atcttggcgt gatggcaggc tacgccaatc agcacagtaa
     4981 tactcagagt aatcgtgtgg gttataaatc ggatgggcgc atcagcggtt acagcgctgg
     5041 gctgtacgcg acctggtatc agaacgattc gaataagacc ggcgcttatg ttgactgctg
     5101 ggcgctgtat aactggtttg ataacagcgt cagttccgat aaccgttctg ctgacgacta
     5161 tgattctcgc ggtgttacgg cctctgttga gggtgggtat acctttgaag cgggaacatt
     5221 tagcggcagc gaagggacgc tgaatacctg gtacgtccag ccacaggcgc aaatcacctg
     5281 gatgggtgtg aaagattctg accataaccg gaaagacgga acacgcattg aaacggaagg
     5341 cgacggaaac gtgcaaacgc gacttggggt gaaaacctac ctgaatagcc atcaccagcg
     5401 tgacgatggt aaacagcgtg agttccagcc ttacattgaa gcgaactgga tcaacaatag
     5461 caaagtctac gccgtgaaga tgaatggtca aaccgtaagc cgtgatggtg cgcgtaatct
     5521 cggtgaagta cgtaccgggg ttgaggcgaa agtaaataac aaccttagcc tgtgggggaa
     5581 tgtcggtgtg caactaggtg ataaaggcta tagcgatact cagggcatgc tgggagtgaa
     5641 atatagctgg taaacagtat aagccgcatg tcgagatggc atgcggctta atattgccaa
     5701 cttcaaaagg cttttaaagc ctccctttta tccgcgatcg cggatatcgc agcgtttatc
     5761 ccgtacagcg gataagatgt gtttccagat tgactttatc ctcactaaag gataaaactg
     5821 ataatatccc cttaagcgga taaatttgct gtggacgtat gacatgatga gctttcagaa
     5881 gatctatagc ccaacgcaat tagcgaatgc aatgaaactg gttcgccagc aaaatggctg
     5941 gacgcagagc gagctggcga aaaaaatcgg cattaagcag gcgacgattt ccaatttcga
     6001 aaacaaccct gacaatacct cgctcacgac attttttaag attttacagt cgcttgaact
     6061 ctcaatgacg ctatgcgaca cgaaaaatgc ctcgccagaa tcaacagaac agcaagatct
     6121 ggagtggtaa tgcccaaact agtcacttgg atgaacaacc agcgggtagg cgagttaacg
     6181 aagttagcca acggcgcgca cacctttaag tatgcaccgg agtggttagc cagccgttat
     6241 gccagaccgt tgtcactttc gctgccattg cagaggggga atatcacctc tgatgccgta
     6301 tttaacttct tcgataactt gttacccgat agcccgattg tacgtgaccg gatcgttaaa
     6361 cgttatcatg ctaaatccag acaaccgttt gatttattgt cagaaatagg gcgagacagc
     6421 gttggtgccg taacgttatt acccgaaaat gaaaccataa cgcgcccgat aatggcatgg
     6481 gaaaagctta ctgaagccag acttgaagaa gtattaacgg cttataaagc agatatcccg
     6541 ctaggcatga ttagagaaga aaatgacttt cgcatctcgg ttgctggcgc acaggagaag
     6601 acagcactgc tcagaatagg caatgactgg tgcattccga aaggaataac gccgacgacg
     6661 cacatcatta aattaccgat tggggaaatc aggcagccca atgcgacgct cgatctcagc
     6721 caaagcgttg ataatgagta ttactgtctg ctgctggcga aagaacttgg gttgaatgtt
     6781 ccggacgcag aaatcattaa agcgggaaat gtgcgcgcgt tagcggtcga acgttttgac
     6841 aggcgttgga atgctgagcg aacggtttta cttcgcttgc cacaggagga tatgtgtcag
     6901 acattcggtt taccttcatc ggtgaaatat gaatcagatg gaggcccagg catcgcgcgg
     6961 atcatggctt ttttgatggg gtccagcgag gcgctgaaag atcgctatga ttttatgaaa
     7021 ttccaggtct ttcagtggtt gattggcgca acggatggcc atgcaaaaaa cttctccgta
     7081 tttattcagg ctggcggcag ttatcgactc acgccatttt acgacatcat ttcagcattt
     7141 ccggtattgg gtggcgcggg aatacacatc agcgatctca aactggcaat ggggcttaac
     7201 gcatccaaag gcaaaaaaac ggcaatcgat aaaatttatc cgcgacattt tttggcgaca
     7261 gcaaaggtgc tgagattccc ggaagtgcag atgcatgaaa tcctgagtga ctttgccaga
     7321 atgattccgg cagcactgga taacgtgaag acttcattac cgacagattt tccagagaac
     7381 gtggtgacgg cagttgaaac caatgtgttg aggttgcacg gtcggttaag ccgagaatac
     7441 ggtattaagt aagagtaaga tatggggttt ggtcgttgtt aatgagcagg acaatcatga
     7501 ccgccaacga ctagattctt ccttggttta tgttggtgcg cataatgtat attatgttaa
     7561 atcgtgtctg agtgatacga agtgattttc cggtagtcgt tattcgttca gggttccaga
     7621 acttgagtct ctcctctttc ataattggcc tgtgcatgcc ggatataggc tggctggtat
     7681 gcagatttgc cgtttgccgc atcttcccag cgttgaagac tcaagctggt aaaccgcgtg
     7741 caaatgagca cgccctcttt ccatcaacac ttcaggcagc ttgcgggaat gagtccactc
     7801 cggtatcaga aatggctacg tctcaatgaa gtcaggcgaa cgatgctgaa tgaacattac
     7861 gatgtcacca cagcagccta tgctgtcggt tacgaaagct tatcccattt cagccgggaa
     7921 tagcttcaat atcaagctga atgattgtga taccaatgtt tccagtaatg cagctgttgc
     7981 attcctgggt actactgtca ccagtaatga cgatacgtta gcgctgcaga gttcagcggc
     8041 aggctctgcc caaaatgtcg gtatccaaat tttggaccgt acgggtgagg tattaatact
     8101 tgatggtgcc acttttagcg ctaaaactga cttgattgat ggcacgaata tactgccgtt
     8161 ccaggctcgt tatattgcac tcgggcagtc cgtagctggt actgcaaacg cagatgcgac
     8221 cttcaaagtt caatatctat aattcaatct taaggtggct atacgtcatc tgaaattgaa
     8281 acagggtgtg tgtggcatgg ctacgcacac ccgagcaatt caccaatgtg aatctcatta
     8341 ttaatgagga gtggtttgta tttggagcgc tatgaaaaat cacataatat ctaaactatc
     8401 aataaacaac agtagttaaa cttactgtta tttaaaatat ttactttgta gcaaggtttt
     8461 cctattaaaa gagaaccaac atttattaca aacgcattaa gtctggaaac ccggcgactc
     8521 gccaatgtat tagcaacatt taccgattgt ctgatgtaac ggttttcccg cttatcctct
     8581 taacacatgg agaatgcatc agatgcaaac tacgcgtacc ccctactcta tttcctttat
     8641 ggcgacagtg ttacttttat tgctctttgc ttgccatagc accgttgcca acgcagctgt
     8701 cgcactgggt gcgactcggg taatttatcc ggcaaaccag aaacaggttt tattgcctgt
     8761 cacgaataat gatcctgcca gtgtctatct cattcagtca tggatagaaa atgcaggtga
     8821 ccaaaaagac acccagtttg tcattacccc accgcttttt tccatgcaag gtaaaaaaga
     8881 gaatacgctg cggataatca acgccactaa tcatcagtta ccaggcgacc gtgaaagcct
     8941 gttctgggtt aatgttaaag ctattcctgc tatggaaaaa gaccagaaga atgagaatac
     9001 attacagctg gcaattatta gccgcataaa aatgttttat cgccccacga acctggcaat
     9061 ggcacctgaa gaagcgcctg caatgcttcg gtttcgtcgc agcggtagca aactcactct
     9121 tattaatcca acgccctact tcattaccgt aacaaatatg aaagcaggaa atagcaatct
     9181 cccaaacacc atggtgccgc caaaagatga agtatcggtg gatatcccgc atgctgccac
     9241 cggggatatc agttttcaga cgatcaatga ttacggtgca ttaactccgc gtattaaggc
     9301 aaccatgcag taagccagtg acctaattaa taataaactc aatcggaatt aataatgaca
     9361 gcctttcgtg ccgcatttaa ggcatatcgt atgcatcagg tgttactact gcctcgcttt
     9421 gcacggctta ccattgccct tagcttagca actgcggtat ttcccgttga cgcagagtat
     9481 tattttaatc cacggttttt aagcaatgat cttgctgaat ctgtcgattt atccgccttt
     9541 actaagggac gcgaggcacc acctggtaca tatcgggtcg acatttattt gaacgacgaa
     9601 tttatgacta gtcgggatat tacttttatt gcagatgata ataacgcaga tctcatccca
     9661 tgcctgagta cagatcttct tgtcagtctt ggcataaaaa aatctgcgtt attggacaat
     9721 aaggaacatt cggcagaaaa acatgtgccg gacaacagcg aatgcacacc acttcaggat
     9781 cgtttggctg acgcatcaac tgagtttgat gttggtcagc aacatttgtc actctccgtt
     9841 ccgcaaattt atgttggcag aatggctcgc ggctatgttt ctcctgatct gtgggaagaa
     9901 ggaataaatg cagggcttct aaactatagt tttaatggta attctattaa taatcgtagt
     9961 aaccataatg caggaaaatc caactatgca tatttgaatt tacagagtgg catcaacatt
    10021 ggtagttggc gactgcgcga taactcaacg tggagttata acagtgggag tagcaattca
    10081 tctgacagca ataaatggca gcatatcaat acgtcggctg aacgtgacat tattccctta
    10141 cgctcacggt taacggtagg tgatagttat accaatggcg atatttttga tagtgtgaac
    10201 tttcgtggcc tcaaaataaa ttcaacagaa gcgatgttgc ccgatagcca acatggtttc
    10261 gctccggtga ttcatggtat tgcccgtggc accgcacaag tgagtgtaaa acagaacgga
    10321 tacgatattt atcagactac tgttccaccc ggccctttta ctattgatga tatcaactct
    10381 gcggccaatg gtggtgatct gcaagtaacc ataaaagagg cagacggcag tattcagaca
    10441 ttatatgttc cttattcgtc tgttccggtt ctccaacgtg ctggatatac gcgttatgcg
    10501 cttgccatgg gggaatatcg tagtggaaat aacctgcaaa gctcccccag gttcatacaa
    10561 ggtagcttga tgcatggact ggaaggaaac tggacacctt atggcggaat gcaaattgca
    10621 gaagattatc aggccttcaa ccttggtatt ggtaaagatt taagactttt tggtgccttt
    10681 tctttcgata tcacgcaggc caatacgaca cttgcagatg gcacccgtca cagcgggcaa
    10741 tcggttaaat ccgtctacag caaatccttc taccagacag gaaccaatat ccaggtcgca
    10801 ggatatcgct attctacgca aggtttttat aacttatccg acagtgccta cagtcgaatg
    10861 agtggttaca ccgtcaagcc tcctaccgga gacagcaatg agcagacaca atttattgat
    10921 tattttaatc tgttctacag taagcgtggt caggaacaaa taagcatctc tcagcagctt
    10981 ggaaattacg gtacgacatt tttcagtgcc agtcgccaaa gttactggaa cacgtcacgc
    11041 agcgaccagc aaatatcatt tggattaaat gtgccgtttg gtgatattac gacttcactg
    11101 aattacagct attccaataa tatatggcaa aacgatcggg atcatttact cgcttttacg
    11161 cttaatgttc ccttcagtca ttggatgcgt acagacagtc agtcggcatt tcgtaattca
    11221 aacgccagtt acagtatgtc aaacgatttg aaaggcggca tgaccaatct atcgggggtt
    11281 tatggcactc tgctgccgga taataacctg aattatagcg ttcaggtcgg taacacccac
    11341 ggaggtaata catcgtctgg caccagtggt tacagttctc ttaattatcg tggagcttac
    11401 ggtaatacga atgtcggtta cagtcggagt ggtgacagca gccagatcta ttacggaatg
    11461 agtggtggga ttattgctca tgctgatggc atcacctttg gacagccgct gggcgacaca
    11521 atggttctgg ttaaggctcc tggcgctgat aatgtcaaaa tagagaacca gaccggaatt
    11581 cataccgact ggcgtggcta tgcaatatta ccatttgcga cagaatatag agaaaatcgt
    11641 gtcgctctta acgcgaattc ccttgcagat aatgttgaac tggatgaaac cgtagtcact
    11701 gtcatcccaa ctcacggtgc tattgccaga gcaacattta atgcacaaat cggcgggaaa
    11761 gtattaatga cgttgaagta cggtaataaa agcgttccat tcggtgcaat tgtcactcac
    11821 ggagagaata aaaatggcag cattgtcgcg gaaaatggtc aggtttatct gactggactt
    11881 ccacagtcag ggaaattaca ggtttcatgg ggcaatgata aaaactcaaa ctgtattgtc
    11941 gattacaagc ttcctgaagt ctctcctgga accttgctga accagcagac agcaatctgt
    12001 cgctaaggaa caatagatta tgaaatacaa taacgttatt ttcctcggtt tatgtctggg
    12061 gttaaccacc tattctgctt tatccgcaga tagcgttatt aaaattagcg ggcgcgtcct
    12121 cgattatggc tgcacagtct catcggattc gcttaatttt accgtagatc tccaaaaaaa
    12181 cagtgccaga caatttccaa cgaccggtag cacaagtcca gccgtccctt ttcagattac
    12241 gttaagtgaa tgcagcaaag ggacaacggg ggttcgggtt gcatttaacg gtattgagga
    12301 tgcagaaaat aatactctgt tgaaactgga tgagggaagc aatacggcct ccggtttagg
    12361 tatagaaata ctggacggaa atatgcgtcc ggtgaaactg aatgaccttc atgccgggat
    12421 gcagtggatc ccactggtac cagaacagaa caatattttg ccttactccg ctcgtctgaa
    12481 gtcaactcag aagtccgtca atccgggact ggtgagggct tcggcaacct ttacccttga
    12541 atttcaataa tcagagtcag atatgaaaag acttcacaag aggttcctgt tagctacgtt
    12601 ttgcgcgtta ttcacagcaa ctctccaggc cgccgatgtc actatcactg ttaatggtcg
    12661 ggtagtcgct aaaccctgca ctattcaaac caaagaagct aacgttaatc tcggggatct
    12721 ttatacgcgc aatctgcaac aacctggttc tgcatctggc tggcacaata ttactttgtc
    12781 attaaccgat tgtccggctg aaacaagtgc agtgacggca atcgtgacag gttcaactga
    12841 caatacgggt tattacaaaa atgaaggtac tgccgaaaat attcagatag agcttaggga
    12901 tgaccaggat gcgacgttaa aaaatggcga tagcaaaacg gttattgttg atgagatcac
    12961 tcgtaatgca cagtttccac ttaaggcaag agctatcacg gtgaatggaa acgcaagcca
    13021 gggaacgatc gaggcgctaa tcaatgtgat ctacacctgg caataacgtc gattcgattt
    13081 tcttagcctt ctttttaaat caatttttta accttaggat atatcatggg taaaacaatc
    13141 agtataaagg tcctgttcgg tatttatctt ctattaatgg caggcaaagt ttttgccttc
    13201 agttgtaatg tcgatggcgg gtcaagtatt ggagctggta caacctctgt ttatgttaat
    13261 ctcgaccctg taatacagcc gggccagaat ctggttgtag acttgtctca gcatataagt
    13321 tgctggaatg attacggcgg ctggtacgac actgatcata taaacctggt acaaggctca
    13381 gctttcgcag gctctctaca gtcatataaa ggatcgctgt actggaataa tgtgacctac
    13441 cccttcccgt tgacgacaaa taccaacgta ttggatattg gtgataagac accgatgcca
    13501 ttaccgttaa aactctatat taccccagtc ggggcggccg gtggagttgt cataaaagcc
    13561 ggggaagtta ttgcccgtat tcatatgtat aaaattgcca cgttaggaag cggaaatccg
    13621 cgtaatttta cgtggaatat tatttcaaat aacagtgttg ttatgccaac aggcggctgc
    13681 actgttgata gtcgtaatgt caccgtgaat ctcccggact ttcctggaag cgcagaaatc
    13741 ccccttggcg tatattgctc aagcgaacaa aagctctcat tttatctttc gggtgccacc
    13801 actgacagtg cccgacaagt gtttgctaac acagcacctg atgcaacaaa agcatcggga
    13861 gtgggtgtct ctctgatgcg taatggaaaa atactggcta cgggagaaaa tatctccctg
    13921 gggacagtaa ataaaagcaa agtacctctg ggtttaagcg caacctatgg acaaacagga
    13981 aacaaggttt cagccggaac cgtccagtca gtcattggtg tcacttttat ctatgaataa
    14041 ttgtgatttt gacattggtc tatgcgctcc attcacgaaa attgcgcgga taattatttt
    14101 gtgaaggcta ttatcctaca cctgtaagaa aatccgcgca caattgaagc cagagtctta
    14161 ttttttatat taaaaaata
//
LOCUS       DAZGCQ010000121        14129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000121 DAZGCQ010000000
VERSION     DAZGCQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.121"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..453
                     /gene="yhaJ"
                     /locus_tag="ACHK07_004149"
     CDS             <1..453
                     /gene="yhaJ"
                     /locus_tag="ACHK07_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8104412.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(504..860)
                     /gene="yhaI"
                     /locus_tag="ACHK07_004150"
     CDS             complement(504..860)
                     /gene="yhaI"
                     /locus_tag="ACHK07_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8104413.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(1102..1467)
                     /gene="yhaH"
                     /locus_tag="ACHK07_004151"
     CDS             complement(1102..1467)
                     /gene="yhaH"
                     /locus_tag="ACHK07_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8104414.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(1760..2746)
                     /gene="yqjG"
                     /locus_tag="ACHK07_004152"
     CDS             complement(1760..2746)
                     /gene="yqjG"
                     /locus_tag="ACHK07_004152"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO8104415.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(2816..3208)
                     /gene="yqjF"
                     /locus_tag="ACHK07_004153"
     CDS             complement(2816..3208)
                     /gene="yqjF"
                     /locus_tag="ACHK07_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8104416.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(3394..3693)
                     /gene="yqjK"
                     /locus_tag="ACHK07_004154"
     CDS             complement(3394..3693)
                     /gene="yqjK"
                     /locus_tag="ACHK07_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO8104417.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(3683..4087)
                     /gene="yqjE"
                     /locus_tag="ACHK07_004155"
     CDS             complement(3683..4087)
                     /gene="yqjE"
                     /locus_tag="ACHK07_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8104418.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(4090..4395)
                     /gene="yqjD"
                     /locus_tag="ACHK07_004156"
     CDS             complement(4090..4395)
                     /gene="yqjD"
                     /locus_tag="ACHK07_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO8104419.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(4433..4801)
                     /gene="yqjC"
                     /locus_tag="ACHK07_004157"
     CDS             complement(4433..4801)
                     /gene="yqjC"
                     /locus_tag="ACHK07_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO8104420.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4948..5331)
                     /gene="mzrA"
                     /locus_tag="ACHK07_004158"
     CDS             complement(4948..5331)
                     /gene="mzrA"
                     /locus_tag="ACHK07_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO8104421.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(5335..5997)
                     /gene="yqjA"
                     /locus_tag="ACHK07_004159"
     CDS             complement(5335..5997)
                     /gene="yqjA"
                     /locus_tag="ACHK07_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO8104422.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(6019..6102)
                     /gene="yqiM"
                     /locus_tag="ACHK07_004160"
     CDS             complement(6019..6102)
                     /gene="yqiM"
                     /locus_tag="ACHK07_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO8104423.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(6342..7118)
                     /gene="exuR"
                     /locus_tag="ACHK07_004161"
     CDS             complement(6342..7118)
                     /gene="exuR"
                     /locus_tag="ACHK07_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO8104424.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQTADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(7248..8546)
                     /gene="exuT"
                     /locus_tag="ACHK07_004162"
     CDS             complement(7248..8546)
                     /gene="exuT"
                     /locus_tag="ACHK07_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO8104425.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            9029..10441
                     /gene="uxaC"
                     /locus_tag="ACHK07_004163"
     CDS             9029..10441
                     /gene="uxaC"
                     /locus_tag="ACHK07_004163"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO8104426.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            10456..11943
                     /gene="uxaA"
                     /locus_tag="ACHK07_004164"
     CDS             10456..11943
                     /gene="uxaA"
                     /locus_tag="ACHK07_004164"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO8104427.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQKRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            12026..12577
                     /gene="ygjV"
                     /locus_tag="ACHK07_004165"
     CDS             12026..12577
                     /gene="ygjV"
                     /locus_tag="ACHK07_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO8104428.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(12581..13825)
                     /gene="sstT"
                     /locus_tag="ACHK07_004166"
     CDS             complement(12581..13825)
                     /gene="sstT"
                     /locus_tag="ACHK07_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO8104429.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3640 a         3566 c         3605 g         3318 t
ORIGIN      
        1 gttatcgcgc cggatatgca ttttcgttcc tcgtcggaga tcaactcgcg caagctctat
       61 acgttaatga acgtctacgt tgccgcgcct gatcacccga ttcatcagga gccggaaccg
      121 ttatctgaag tgacgcgtgt gaaatatcgt ggaattgcgg tggcggatac cgctcgtgag
      181 cgcccggtgt tgaccgtaca gctgctggac aaacagccgc gcttaacggt gagcacgatt
      241 gaagataaac gtcaggcatt actggcgggg cttggcgtgg cgacgatgcc gtatccgatg
      301 gtcgaaaaag atattgcgga agggcggttg cgtgtcgtca gcccggaatc gaccagcgag
      361 atcgatatta ttatggcctg gcgtcgtgac agtatggggg aagcgaaatc ctggtgtctg
      421 cgggaaattc ccaaactttt taacggaaaa taatatttat tattgttaat aaaaaggggc
      481 gaaatatcgc cccaattcta agattaattt gaaccaaact tcggatcgtt tccgtaacga
      541 ttgctgccag aagtaccttc tgtgcagaaa aaaaccagaa gcacgagcca gccaataaac
      601 ggaacaaaaa agagcaaggc ccaggcacct gaacggtcgg tatcgtgtaa gcgacggata
      661 gcaagcgcaa ggacgggaag gaaggttgcc agcaaataaa gcatagacag atatggaagc
      721 tccagaccta aaatcaattg aatgacattg ataatagcgc cgacgatggc gttaatcaga
      781 gtaaacatcc agtactcttt acgacgtgcg cgaccagaga aaccaacata attttttaat
      841 acggataaat accactgcat aaaaacatcc ctttaattaa aagtgacgaa agtctattcc
      901 gggtattgtg taaatgctat tcgtatcttt ctttttcgga tgttataaag gttttgatat
      961 ttaagataaa tacatggtta ttttttatag gtaatttata taacgacatt gaaatatttt
     1021 aaataaacat tagtaaatta ttcattcatc aacatgatga taaaagccgg atgagtcaga
     1081 catccggcga gcagatttgt tttaaggttc cagttttgga tcaggtccaa agcggttttc
     1141 gcctggcgta cccgcctggc agttaaagac gatgataata agccagccga taaacgggat
     1201 taagaatagc agtgcccacc acgccgagcg gtcggtgtcg tgcaggcggc ggaactgaac
     1261 tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc gtcaggatgc cttcgccacc
     1321 agcgcgttgc cagcctaaca ttttatccag cagccccagc acgaacgtaa agatgatgtt
     1381 gaccagaata aacatccagt actctttgcg ccgcgcacga ccccggaaac cgacataatt
     1441 ttttagtact ttcagatacc agtccatttt tgcctcaatt ttgctcaatc acttgttttt
     1501 aaagcagcaa attaagaata gacgtgaatg aaatggcagt aaatattcgt tcaaggaatt
     1561 aataaagtgc tgtgaaggcg cgctaagact gatgacaagc gcaaaattgc cagatgcgct
     1621 acgcttatct agcctgcgcg gtcattgcaa catattgaat ataaaagttt tgtaggtttg
     1681 ataaggcgtt catgccgcat ccgacatgaa caacatgcac ttagctaatt atcagaggca
     1741 cctcgtaagg tgcctttttt taaccgaagc gaacatctcg tccatgcggt tcatcgagat
     1801 cctgccacgg accaattgaa ataatccccg tagggttgat ggtcttatga ctgcggaagt
     1861 aatgattacg gatatgatcg aaattgactg tttcggcaat tcccggcatc tggtagatat
     1921 cacgcaggaa gccatacaga ttaaggtaat cgctgatgcg gtgcttatca cacttgaagt
     1981 gggtcacata cactggatca aaacggacca gcgtggtcca caggcgaata tcggcttcgg
     2041 ttagctggtt gccggtcagg taacgatgct gacctaaaat ctgttccagt cgcgccagcg
     2101 attcaaacac tttctccacc gcttcatcgt aggcttgctg gctggtggca aaaccagctt
     2161 tatacacgcc gttgttaacg gtgtcataaa tccagccgtt aagttcgtca attttcgttt
     2221 gcagggctgg tgggtagtaa tcacccgctt tcgcgcccag cgcatcaaac gcggtattaa
     2281 acatgcggat gatttctgct gattcgttgc tgacgatggt gtggttcttt ttgtcccaca
     2341 gcacgggaac agtaactcgt ccgctgtagt gtggatcggc gtggagataa agctgataca
     2401 aaaattcatg ttgatagagc gtgtctccgg ttgctcccgg aaaactgtcg tcaaaggtcc
     2461 agccgttttc cagcatcagc gggttcacta cggaaacgga aataaacggt tccagtcctt
     2521 tgagtttgcg catgatcagc gtgcggtgcg cccacgggca ggcgagtgaa acatagagat
     2581 gataacgatc tttctctgcg ataaaaccgc ctgtgccagt ggggcctgga gcgccatcgg
     2641 cagtgagcca gttacgaaat gcggaagctg aacgttgaaa tttaccgccg gtagatttgg
     2701 tatcgtacca ggtgtcatgc cagacgccgt caatcagttg acccattttt ctctcctcca
     2761 atagcaaaaa cgaggagctt cctcctcgtt ttttatcgtt aagtatagcg cgtgcttacc
     2821 actttttatt cagcaggcgg tcgatgctat acgcgcccgg accggtaatt gccagcagca
     2881 ggaatccgcc agaaattgtc aggtttttca tgaacatcag cgagttgacg ccttcagcaa
     2941 agttgctgtg aaataaaaat gccgtcagca gcgtaaagcc cgcagtaaac agggctgtgg
     3001 tgcgagtcag gaaaccgaac aggattgcca gaccaccacc aaactcaagc agaatcacca
     3061 gcggcagcat aaaacccggg acgcccatcg cttccatata ttgttgggta cccgcgtaac
     3121 cagtaatttt tccccagcct gcggtaataa acagaatcgg cattaaaatg cgcgctacca
     3181 gtacaccaac atcttctaat tttttcatca tactctccac tgcgccactc aggctgctga
     3241 ttgttttttt gtgcaagggc gcggtattag cgtcgttgct gtcgatggag agaatcataa
     3301 acgtggtgaa tgatgattgt tagcaaggaa aactgtcaaa aatcttcaaa aaatttgatg
     3361 ggtaaggccg gaatggctcc ggccagagag aagttaaccg cgaagctgtt gctgcttgag
     3421 ggtcgtttta accagacgcc aggcgctcca tacgccaaaa ccgcgtctgg cccagcggac
     3481 cagcatatta ggatggcgaa tcgtccagat cgccatcacg ctactgccaa ccagcgccca
     3541 ggagcgcaga cttagcagca tattccagcg acgatcgtaa gcgcctgttg cctccagcca
     3601 ttcacgacga ctggcagaaa gatccagccg ttgttgctgg atctggctaa gcagttgcgc
     3661 cttacgtcgt tcacgttcga ctttactgct cacgggactc ctcctcgagc agctgccgat
     3721 cgtttgccag ctcatggcgt gtatggcgca gcaaggtgga tttacgcgat ttacgtaacg
     3781 tccagatacc gccaatcagt gccagtagca gcaacaccac ggtggtggca atcatcgcat
     3841 tcaggcgata ttgcggatca accgcccaaa taattagcac catcaggctc ataagaccaa
     3901 atgcagcgaa aagcatcgtc aggcccagca tcagtaaaag ttgaaagaga ttagcttttt
     3961 cctcttccag ctccaccacc gccagccgca gacgtgtttc caccatttca accatgatgg
     4021 aaacaattcg ctgcccgatg cccagaacgc ttttaccggg cccttgcgcg tgatgagtgt
     4081 ccgccataat taacgacgcg acagcagaac gccgagcact acaccgattg cagcgccaat
     4141 gcccacgccc gtccatggat tttcacgcac atactcatcg gcacgcgccg ccgcgacacg
     4201 ggtttgtttg gcgatggcat caccggtttc acccaggcga taacggctct gtttcagtgc
     4261 ctgctccgct ttgctacgaa tcttactcaa ctcttctttc gacttctcgc cagatgagct
     4321 aagcacctct tccagcgtat cggaaaggga tttcaactca gcacgcagat gttccgtagt
     4381 gtgttctttc gacatagttt tctccaggtg agtaagtagt gactattgtg agttagtagt
     4441 cgcgcgcttc cagctttttc agctcttcct gcgcttctgc cagtttccgt tcgcgtttgg
     4501 caatcttatc ggcatcgcct ttttgcttcg cctcggctaa atcttgctgg cgttccgcca
     4561 cctcatcttt ctgctttgcg attttcttct gatgatcggc acgcagctgg ctatctgaac
     4621 agttggcccg gacttcactc agggctttat tcagaccgtc aatacgattc tggttttggt
     4681 gtttttcggc atagctgatc tccttaagga tattttgctc cttttcctga cacagggtag
     4741 tggcataact accggcacta agagcaaaaa gagaaacagc taaagcgatg cggtatttca
     4801 ttcttgaacc ttccattgtg attcggacct attacaaagg aacgattgtc cagacaacga
     4861 agcggtagtg ttaaccaccg cctcattaat catagacagt tacggtgaaa tcaccaaagt
     4921 gagtgatctg tttcggataa caggaagtta tccgaagcga tgtgagttat cccgtagccg
     4981 ggtcagccac tgcatagcct gactgttatt gtcctgctgc gcaatgatgt agccttgggg
     5041 caatgttctg tcgaggaccg cttttgcagc ggcgctctgg tcgctggagt caaaggtaat
     5101 taacagagtg tcgtttttgg gggtgatact tttgaaagga atgccatgag cgtcaaggtg
     5161 atgccagatt gaaaaaccgt ctggcatcgt tgtgccttga tgaacggcac gaatcgccag
     5221 cgtagactct tgctggcgaa ccgcggacca ggccagcaac agcgtgccga ccagaagtaa
     5281 aacagcgccg gaccaggcta gctggcgaag cgacatgcga ggtatttgca tcccttaccc
     5341 ccgatttcca tatttctttt tccataacac gaccagagaa cctgccaggc caaacaccag
     5401 cagcaccacc gggagcagca tcaggcatga catcagctgg tcctcgtact ttaaaaatac
     5461 cggcgttttg ccgagcatgt aacccagagt tgtcaggatc aatacccaca gcagaccgct
     5521 catccagttg aaaaactgaa agcgcgcgtt attcagccct gataacccgg caatcgtcgg
     5581 cagcagtgtt ctgacaaacg caataaagcg accaattaac agcgccgata aaccgtgttt
     5641 atgaaaaaga tggtgtgcgc gttgatgata atgcgcgggt aaatgagata gccagttttg
     5701 tacggtgcgg gtattgccca gccatcgccc ctgaatatag ctgacccagc agccgaggct
     5761 ggcggcaacg gtcagcagca gaatcgtttg cggatagccc atcgcgcctt tcgcaatcaa
     5821 cacgccgacc aataccagta aactgtcgcc cggtaaaaag gccgccggaa gcaagccgtt
     5881 ttcaaggaac aaaattacaa acaagacaaa atacaacatg ccaatcatcg atggattggc
     5941 cagggtttca aaatcctgcg cccacagggc ctgcagcaat tgggtcaaaa gttccattca
     6001 ttattcctgg tgaatcggtt aacgccacgc tggttttaaa agatgcacta ccgcaatgtt
     6061 attgctgttt tggtatgttc tgcgggtttg atacatcaac acagcctgaa ctattccctg
     6121 ttaaatcgtg agataagaaa gcattcaaaa acataaattc aaattgaatc taattgtaac
     6181 aaaggtcaac gttgttcgtt atcaaaatta catgtccgtg gagtctgatt ttgctgaatg
     6241 caggggaggg tggcgagagg atttacacag gctgacactt tatacttttc gcttaccgac
     6301 gagacaaaca ggcgcattgc agcgcctgtc ggacaggaat ttcatttact gccgctggtg
     6361 gctgtatcga gatgcaccac cgggttttcg gcgaatagat agcggtcggc attgaactcg
     6421 aagtcgtcgc tggtttcgtt gaataacatg atcttggtgt tttccaggtg ctgccacatt
     6481 gccagcttag cagcatgagg atctttgcga atcagcgcct tgaggatttg atcgtggtca
     6541 tcacaccagt tatcgacggt acgggaatca atgtgttcgt gcagtttttt ccagtacggg
     6601 ttatggctac gctgggtcca cattttttca acgatagccg ccagggcgga gttctgcgtc
     6661 gccagggcta cctgaatatg gaactgcaaa tcccactcgg aatcacggaa gcattgttca
     6721 ccgcgtgcct gctcctggat ggccatcaat ttcatgatgt cctgtttcgt tacctgagtt
     6781 gccgcgaact cggcaatatt actttcgatg agctggcgag cctggagtaa ctcaaacgga
     6841 ccgtaattgg cgaattccat attattgtcc gccgtctgct gatggcgcgg ctggttggaa
     6901 accacgtgaa tacctgaacc tttacgcact tcgacatagc cttcaacttc cagcatgatg
     6961 atggcttcac gaactaccgt acggctgacg ttcttttcat cggcaataaa gcgctcggca
     7021 ggcagtttat cacccaccag atagacgccc tgttcgatgc gctctttcag gtcagcggca
     7081 agttgttgat acaaacggcg tggttcagtg atttccatat gcgctccagg catgatacgg
     7141 cagactatat ttgttatacc acttttgcga gtttagctct actcgcagtg atgaaaaagc
     7201 cgcctgagaa ggcggctttg aagggaaact aaaacttaca cgaagcgtta atgttgcggc
     7261 gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg caacacggtc
     7321 cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag cgggctgaag
     7381 ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc cagccatgcg
     7441 gacatcccgg ttaaaccgtt tgccgttgcc acttcgttac gaccaaacac gtcagaagag
     7501 agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca cagcagcata
     7561 attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat cagcacagca
     7621 cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca acgctggaac
     7681 agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat cggcatccag
     7741 gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa cagcgggatc
     7801 cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagcgc gataccccag
     7861 aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct cacctggtgc
     7921 tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg cttctggtcg
     7981 cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa tgcaccggag
     8041 atgataaatg ccatctgcca gctgtgcatc acgattgccc ataccaccag cggcggcgca
     8101 atcatcgcac caatcgaaga acctacgtta aagtagccta ctgcgatgga acgctctttc
     8161 gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc ttccgcggca
     8221 ccgaccgcac cacgagcaac agccaggcca ccccagctac ctgccagcgc ggttgcacca
     8281 cagaacacgg cccacagcac agcaaacatt gcataaccga ttttcgtacc cagcacatcc
     8341 agtacataac ccgctaccgg ttgcatgacc gtataagcag cagaataggc tgcgatgata
     8401 taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc agctgccgcc
     8461 acagtgttac gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc gatcatatac
     8521 caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata tccgcaggaa
     8581 gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta accgaaaaaa
     8641 aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt ccgtaccggt
     8701 taacccgatt catctaacaa atgagtgaat cggcaatttg cattccgtcg acttatgatt
     8761 tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca tcacaaatgt
     8821 gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg cggcctgcag
     8881 cgaaatttac cactttgaga gtaatttttt taactacgtt tattgatcta actcacgaaa
     8941 atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc ataagcaagc
     9001 tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag atttcctgtt
     9061 agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc cgattttcga
     9121 ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta aaaacctgta
     9181 tgacatctgg ttgaagggcg atcactacaa atggcgcgct atgcgtacca acggtgtggc
     9241 tgagcgtctg tgtactggtg atgcgtctga ccgtgaaaaa tttgacgcct gggcggcgac
     9301 tgttccgcac actatcggca acccgttata ccactggacg cacctcgaac tgcgtcgtcc
     9361 gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct ggaacgaatg
     9421 taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc agatgaacgt
     9481 gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg cagagatcgc
     9541 caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca aagcctttaa
     9601 catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt ccgataccga
     9661 cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc acttcgccgc
     9721 tcacggctgt aaagtgtctg accacgcgct ggatgtggtg atgtttgctg aagcgaacga
     9781 agcggaactg gacagcattc tggcgcgtcg tctggctggc gaaaccctga gcgagcacga
     9841 agtggcacag ttcaaaactg cggtgctggt gttccttggt gccgaatatg cacgtcgcgg
     9901 ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc agttcaaact
     9961 gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg aagagctgtc
    10021 taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc tctactgcct
    10081 gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg gcgaaggtat
    10141 gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag acggtatgga
    10201 acgtcagatg acccaactgg cgcagctcgg tttgctgagc cgctttgtcg gtatgctgac
    10261 tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca ttctgtgcca
    10321 gatgattggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc tgctgggtga
    10381 gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg aactgaacta
    10441 aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt cgcggtcgct
    10501 ttagcggatt tggctgaagg cacagaagtc agtgtcgata accagactgt tacgctgcgc
    10561 caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg ggccaacgtc
    10621 atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg ggaacacgtt
    10681 cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta tcaacctgat
    10741 tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg tcgcgctaac
    10801 ggcgatgtcg gggtgcgtaa tgagctgtgg atcctgccaa ccgtgggctg tgtcaacggc
    10861 atcgcgcggc agatccaaaa gcgtttcctg aaagagacca acaacgccga aggtaccgac
    10921 ggcgtgttcc tcttcagcca cacctacggc tgctcacagc tgggcgacga tcacattaat
    10981 acccgcacca tgctgcaaaa catggtgcgc cacccgaacg caggcgcagt gctggtgatt
    11041 ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgctggg cgatatcgat
    11101 cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga ggccgggatc
    11161 gagcatttgc atcagttgta taacgtgatg cgcaacgata aacgcgagcc aggcaaactc
    11221 agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg tattactgct
    11281 aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac taccgtactg
    11341 accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg ccgcgacgaa
    11401 gcaacgtttg aaaaactggt cactatggtc aacgacttca aacagtactt tattgcccat
    11461 gaccagccga tctatgaaaa cccatcgccg gggaacaaag cgggcggtat caccacgctg
    11521 gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt tgacgtgctg
    11581 cgttacggcg agcgtctgaa aactccgggg ctgaacttgt taagtgcgcc gggtaacgat
    11641 gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt cagtactggt
    11701 cgtggcacgc cgtatggtgg atttgtgccg acggtgaaaa tcgccaccaa cagtgaactg
    11761 gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca cggtaaagcg
    11821 atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa cggtaagcaa
    11881 acctgtaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg cgtaacgcta
    11941 taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc gccgtttgct
    12001 tttccatacc tgctaaggat ttatcatgac cgcgtattgg ctggcccagg gcgtgggtgt
    12061 catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc gcttcaaaaa
    12121 gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg gcacctatcc
    12181 cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac gcacgcgcag
    12241 cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg cgaagttcca
    12301 tcatcctgtc gaactattgc cggttatcgg cacgattgtc agtacctggg cgctgttccg
    12361 ctgtaaaggg ctgaccatgc gctgcgtgat gtggttttca acgtgttgct gggtgattca
    12421 caacttctgg gcggggtcga taggcggcac gatgattgag ggtagttttc tgcttatgaa
    12481 tggcctgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc cgtttaaagt
    12541 agagaaaacc ccttccgccg tagacgaaag gggttaaaca ttaattacgc agggcgctat
    12601 ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg tcagttgaag
    12661 agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg acggcaacca
    12721 cctgcatggc gatatcgttc gaaataccga acatattaca ggccagcggg atcagcagca
    12781 gagaaccacc cgccacgccg gatgcgccac aggcacacag agaagccact acgctcaaca
    12841 gcagcgctgt gggcagatcg accggaatac ccagcgtatt aaccgcagcc agcgtcaaca
    12901 cggtaatggt gattgctgca cccgccatat tgatggtggc acccagcgga atagaaacgg
    12961 aataggtatc gcgatccaga ttcagctttt cacacagcgc catattcacc ggaatgttag
    13021 ctgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac agcagcacca
    13081 gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc accagcgcca
    13141 ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac agtgtggaga
    13201 aaccggtggt tgccagggta gaagaaacca gcccaaaaat accgatcggt gcgaagcgaa
    13261 tgaccagttt caccataaag gtgacggcat tcgacatatc gttgaccagg tttttggtgg
    13321 tttcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg atcccgatgt
    13381 agttaccttt cagcagcgcg tcaatggggt tggaaaccat gctcattacc agtccgcgca
    13441 tcacttcgac aatgcctgac ggcggcgaaa tatcacccgc gctactggac aagtgcaggg
    13501 tagaagggaa ggcaaagctg aagactactg cggccagagc tgcagagaag gtgcccagca
    13561 gatagaggaa caaaatagga cggatattgg ttttctgccc gtgctggtgg ttagcaatag
    13621 atgccattac cagcatcaac accaggatgg gggcaacagc tttcagtgcg ccgacgaaca
    13681 aagtacctaa cagaccaaca gcttccgccg cgggttttga gatccatgcc agaagaatcc
    13741 ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga cgccggaata
    13801 gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttgtc gcacatccct
    13861 ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg tcgagtataa
    13921 ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaggt catgttttgc
    13981 aacttaatgg ttacatttat ttgacataaa cagagggaag gactggctac taaggctgat
    14041 tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc tttaatgtgc
    14101 tatgccatat aaagtcactg gcacagggg
//
LOCUS       DAZGCQ010000122        14096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000122 DAZGCQ010000000
VERSION     DAZGCQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.122"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            255..2492
                     /gene="nrfB"
                     /locus_tag="ACHK07_004167"
     CDS             255..2492
                     /gene="nrfB"
                     /locus_tag="ACHK07_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO8104430.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHK07_004168"
     CDS             2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHK07_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO8104431.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5452..6342
                     /locus_tag="ACHK07_004169"
     CDS             5452..6342
                     /locus_tag="ACHK07_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO8104432.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQRSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6525..7286
                     /gene="envY"
                     /locus_tag="ACHK07_004170"
                     /pseudo
     CDS             6525..7286
                     /gene="envY"
                     /locus_tag="ACHK07_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            complement(7332..7408)
                     /locus_tag="ACHK07_004171"
     tRNA            complement(7332..7408)
                     /locus_tag="ACHK07_004171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7372..7374),aa:Arg,seq:tct)
     gene            7651..8283
                     /gene="fimZ"
                     /locus_tag="ACHK07_004172"
     CDS             7651..8283
                     /gene="fimZ"
                     /locus_tag="ACHK07_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO8104433.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8286..8801)
                     /gene="sfmF"
                     /locus_tag="ACHK07_004173"
     CDS             complement(8286..8801)
                     /gene="sfmF"
                     /locus_tag="ACHK07_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO8104434.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(8812..9819)
                     /gene="fimH"
                     /locus_tag="ACHK07_004174"
     CDS             complement(8812..9819)
                     /gene="fimH"
                     /locus_tag="ACHK07_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8104435.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9832..12441)
                     /locus_tag="ACHK07_004175"
     CDS             complement(9832..12441)
                     /locus_tag="ACHK07_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO8104436.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12472..13164)
                     /gene="fimC"
                     /locus_tag="ACHK07_004176"
     CDS             complement(12472..13164)
                     /gene="fimC"
                     /locus_tag="ACHK07_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8104437.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(13384..13926)
                     /gene="fimA"
                     /locus_tag="ACHK07_004177"
     CDS             complement(13384..13926)
                     /gene="fimA"
                     /locus_tag="ACHK07_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8104438.1"
                     /translation="MKLRFISSALAAALFTATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3708 a         3530 c         3336 g         3522 t
ORIGIN      
        1 ctgctcacct ccaggcactt ccactacatc atgacctgat cggtgccacc actcctcttc
       61 actatgacgt taaccccccc tccttgacac cacgcttttg tctacattct acgcaaacgg
      121 caacttttat aaccagtgta aaaataacgt gccgcaatag tcatacctat taatggtaaa
      181 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
      241 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
      301 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
      361 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
      421 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
      481 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
      541 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcagcgtg
      601 atgttgacga agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg
      661 gccccaccag caaagccgac tgtctgaaca acgtgctgga cgccatcacc cagtttgagc
      721 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
      781 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccgg
      841 tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
      901 cagagctgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg
      961 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc tctgttagct gacggtgacg
     1021 gtattgcttt cgacgtgcag agtctgactg aagattacga cattggcttc cgcctgaaag
     1081 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
     1141 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     1201 tccccgatac cttttcgact gcggttcgac aaaaatcccg ctggatcatc ggcattgttt
     1261 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     1321 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     1381 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     1441 ttttcagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggt ttgatggtta
     1501 accgcatcgt gcagcgggtg attttcgtta ctggctacta cggcctgacg caggggctgc
     1561 tttccgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgt
     1621 taaaacaggt acttcaacac ggcgatccac gtcgcgtggc gtgggataaa acaacgcatg
     1681 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     1741 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
     1801 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcaa ctggcacagg
     1861 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccttcct
     1921 cgctgattgc cgaaatgccg gcctccgtgg cgctgcatta tgcggtactg ccgctgcgtc
     1981 tggaaaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     2041 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     2101 tcacggggtt acgtcactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     2161 acaatgcggt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc tggcggcaat
     2221 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     2281 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     2341 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     2401 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     2461 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     2521 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     2581 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     2641 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     2701 cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt
     2761 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     2821 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgacagtt
     2881 gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt
     2941 gaagtcgggc agaatgccct gcggctggca cagttacctg tcgccagagc gcaactgaac
     3001 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     3061 gcaatctacc tgaaacaatg gtcccaggca gatacgctat acaatgaagc acgccagcag
     3121 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     3181 gacgatcgga tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt
     3241 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     3301 gaaaataagc cgctgtttac cacggacgca caagagaaaa gttggctcta tctgttatct
     3361 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     3421 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     3481 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     3541 agcgtggcga cccgtaacaa ggctgaagct ctgcgtctgg cacgcttgct gtatcagcaa
     3601 gaaccggcaa atcttacccg cctggatcaa ctaacctggc aactgatgca gaacgagcag
     3661 tcacgggaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtatc
     3721 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg
     3781 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
     3841 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     3901 tcgccttcct acgatgccgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     3961 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgaa cgcctggcaa
     4021 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     4081 tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc
     4141 gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc tacaacaggc agaacaacgt
     4201 ggactgggaa gcaatgccct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag
     4261 ccggaactcg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct
     4321 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg tcccggccgc ggtgagtgat
     4381 ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc gcttggttac
     4441 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     4501 gggcttccgg acgatccggc actgatccga caactggcct atgtgaacca gcgtctggat
     4561 gacatgcctg cgacgcagca ctacgcccgg ctggtgattg atgacattga taatcaggcg
     4621 ctgataaccc cattgacgcc agagcaaaat caacaacgct tcaatttccg ccgtttgcat
     4681 gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt ctatcggctt gcgttccggc
     4741 gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc
     4801 tacgggcaac tggaagccga ataccgcatc ggacgcaata tgctgctgga aggcgacctg
     4861 ctctcagttt atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg
     4921 aaaaatccga tgtccggcac cggtctacgc tggaagccgc tgcgcgatca gatctttttc
     4981 ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg
     5041 cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt
     5101 tccggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg ccaggatatt
     5161 caggcgtgga cggcagatta tcgcgtcagc tggcatcaga aggtagctaa cggacagact
     5221 attgagcctt acgctcacgt tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg
     5281 cagcttggcg gtgtcggggt ccgctggaat atttggaccg gcgagacgca ctacgacgcc
     5341 tggccgcaca aagtcagtct cggcgtcgag tatcaacata cctttaaggc gattaatcaa
     5401 cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag
     5461 ttcattttcg tattgctgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt
     5521 attatctggc aaccacaaaa ccgagatagt caggttaccg atacccagtg gcaggggctg
     5581 atgagtcagt tacgtttgca aggtttcgat accctggttt tgcaatggac ccgttacggc
     5641 gatgcattta cccagccaga acagcgcacg ttattgttta agcgtgccgc agctgcgcaa
     5701 caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag
     5761 aaacagtcgt ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc cgatctccag
     5821 caagccagat tatggagcgc cgcgcctggc ataacgccgg atggctggta catcagcgcg
     5881 gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc agcctttgct aacatggtta
     5941 aacaacgcgc agcggctgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt
     6001 ttcgccggaa acatgtcgcc cgatggctat cgccaactgc tggaacacgt taaagcaacc
     6061 ggcgttaatg tctgggtaca ggatggcagc ggcgtggata aactgaccgc tgaacagcgt
     6121 gaacgttatc tacaggccag cgccgattgc caaagatccg ccccagcaag cggcattgtt
     6181 tatgaactgt ttgtcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca
     6241 gaaattgcct cgctgttagc gaaacgttcc tcttgcggta aagacactct ctatttctct
     6301 ctgcgctatt tgcccgtcgc gcacggcatt ctcgagtatt aaatctcctc caggtaagtc
     6361 gggtacgacc tggcttacct ctttcgctct cgtcagaatt ccatcaagat taattgctaa
     6421 aaagcgtgct aaagaaagaa gatcgttatc ggcgaacggg ccgatcactc atgcaggaag
     6481 catagatcct ttgagcggag tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca
     6541 gtgaaccttg cgtggtgatc ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg
     6601 ctacgtttac ccttccgaaa aactacctgg ccgccttcgc gtgcaacaat aacgtcattg
     6661 aactctcaac gttaaatcac gtattaatca cccacatcaa ccgtaacatc atcaacgatt
     6721 atctgttgtt tttaaataag aacttaacct gtgtaaagcc ctggtcgcgg ctggctaccc
     6781 cggttatcgc ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca
     6841 agcagcaacc cagcaaaccc tgcgaagcgg agttgacgcg cgcattgctt tttaccgtat
     6901 tgtctaactt tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca
     6961 gcgtccgcga cagcgtctgc cgcattattc aaagcgatat tcagcattac tgaaatctgc
     7021 gaattgtcgc cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg
     7081 aaaataccag ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt
     7141 tattgatgga taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt
     7201 cgtactttat ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca
     7261 aacagcggca aaaagtgatg tggtgaaggg caaaccggaa acggataaga cgggcataaa
     7321 tgaagaagaa atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg
     7381 ttgctctatc caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa
     7441 ttatacggtc aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg
     7501 accgcgtctg attttctgat ttatttcgct atagcggcaa acaaatgcac accgctgcgc
     7561 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga
     7621 aaattgtccg ctcctatgag actggtaact atgaaaccaa cgtcggtgat cattatggat
     7681 actcatccta tcatcagaat gtctattgaa gttctgttgc aaaaaaacag tgaattgcag
     7741 attgtcctga aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt
     7801 gatttaatca ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
     7861 atcaaacaaa tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt
     7921 tatgctggca gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag
     7981 aatgatattt ttcatgccgt tcagatgatc ctctccggat acactttttt tcccagcgaa
     8041 acgctcaact atataaaaag caataaatgt agtacgaata gttcaacgat cactgtgcta
     8101 tccaatcgtg aagtgaccat attacgttat ctggttagcg gattatctaa taaagaaatt
     8161 gccgataagt tattacttag caataaaaca gttagtgcgc ataaatctaa tatttatggc
     8221 aagctaggtt tgcattcaat tgtagagctt atcgactacg ccaaattata cgaattaata
     8281 taatattaat tataattgat cataaatatc gcatccgctt tcgccacacc tggctgaaca
     8341 ccgctggcta acgctcgata attggcaaaa aagtttagtg tgacattgcc atttgcatct
     8401 acttcctcag tcgggctcgc ctcccccagt gcgagccggg agcgatcgct attacgtaat
     8461 tcgatggcaa cggtttgtgc cattgcggga tcatccagag ccagcaggtt ggtatcggat
     8521 gccggcgttc ccgtaaataa aatcgcaact gaacccggag gacatccctc cagccgcagg
     8581 ctaaaaggga cgagtgccgt ggtatcgcca gcgttcagta attgtgtcgt aggccatctg
     8641 cctaaatcta ccgtcttatc cttatccgct gtgtttacgg tacaagagaa atcaaccacg
     8701 ttaccgtgca aattgatatt aatcgttcct aaagggtcag ctgcccatcc actggaactc
     8761 cacagtaacc cgcagaaaca gctaaagaat actcttctca ttctccgtac ctcattgata
     8821 atcgacgcgt aaatacccca gcgcgctaaa cggcccctcg gtcggttttt gaccggtaat
     8881 actgataggc caggcgcgaa gtgtgacatt ggctgccgca gctgcatcca gacggaaagg
     8941 aataacgcta ttgagatcgt taggcgtgat cggcgtatcg ttctgatcgg cgacaataaa
     9001 acctaaatcc tgattgtccg acaccatcgc ctgaccagaa acggcactgg cttccagacg
     9061 cattgttaaa taagcctgcg cagcaacatt cgtacatttg accgcaatgc tcttggtttg
     9121 cggcatgaca ccagcaggtc gattacccgg ccctgccgca ctaaataacg atgcgccgat
     9181 atcaccaaaa tcaaattcaa caatctgccc ggcatttaat tcgcagtttt gcggtacttc
     9241 aacccggcca ccaaaactaa tggtataaac aggtgtaacc aacggatcgt aaggcgttga
     9301 tgtcacatac acggtaaaca tcacctgtct ggggatctcc accatgttaa taaagggccg
     9361 aataaccttt aaacgaaata tcaaacgaga atccatcacc ccgaacggaa ggttttgcga
     9421 aacgttagga tctgttccca tacgaatata attttgcggg ggaaattgaa tatccatcac
     9481 gctatcaacc agactcatcg cacctaatag ataatcattt aattgcatat atttataatt
     9541 atcgatagtt tcctgaacaa taaagttggt gacataacta cggtatgtat aattcaccag
     9601 cgtgccgggt gggcaaattg ctgacacacc tacccagccg gatttttccg gtaaaacaac
     9661 aatctgccct ggctggttat tggtgctatt aaagacattt gagagatcat agtgttcatc
     9721 ttctgctacg ccagtactat ttgcacaaac agtagcccag gatatgtttg ccagacacaa
     9781 acatgccatt gccaataata atctgcagat tatcttcatt gataatttcc tttatgagcc
     9841 aggatgtgtg caaactgccg aaataacagt gacggcttgc tgtaagcttt gcttcggcaa
     9901 tacatagtgc gcaatgcagc gtgagttcgc gccgtctccc cactgaacca gtaattcacc
     9961 agacaatggc gcaccactta aataaacttg cccgtcatca cccaccatac tggttattcc
    10021 ggtactgttt tcccgtacca gtgatccaaa cggcaccggt tttccgccct gggtaacggt
    10081 aatgagcgcc cgcacgccta tgcgggtatc aaaattggca cgaaccaacg cgccttgcgt
    10141 cggcacaacg ctgctaatat ttttttcaac atcgatggaa ttccccatcg tattggtatc
    10201 aagcgcgata cggttatacc gataaaccgt ggcatacggc atcaccgcat agccgcgcca
    10261 gtcggttaaa atgccagttt gattttcaat gcgtacaccg cctgcgccag gcgctttaat
    10321 caaaacattg gtatccccta aaggctggct cagcgttatg ccattttcat gcccgaccac
    10381 tccgccagac agctgccagt taacgtcatg ttgatcgcgg tcgtagttat acccgactcc
    10441 cagcgtaccg taagcggcct gccagtttgc cgtggcgctg cccgtatacc cattattcga
    10501 ggtatcaccc tggcttacgt gataactcag gttgtggcct tccagtaagg tcccacctac
    10561 acctgccagc cagctatttt gcccgttgct gttacggttg gcgttaaagg aggcataagc
    10621 gcggtcgagc gcattttccc gggtgtagcg acgtttggtc aaaacattga aaggcactga
    10681 aacattaagt ccgacaatac gttcgttatc ggggatccct acagattcat tccacgaaaa
    10741 tgagagcgaa taactgatgc caacccagct gctggtatac cccacctgat accacgtatc
    10801 tgaatccgaa gtattccagt atttttgatg ggtaccagaa atataaagcg agccaaagtc
    10861 attaagtgat tgtgaaatat ttaactgcaa acggtcttta cggctaaagc gtaaattgtg
    10921 gtaattcacg attatcggtt catcccgatg ctcaccgtcg taatcgtaat catattcgta
    10981 cccctccatt cgacgatacg caacatcatc aagggtataa aaaccttgtg tcgaatagcg
    11041 gtaacccatt aactgaaaat tggtgccgaa ggtgttcatc gatttcgcat agaggaagcg
    11101 aatggaatcc ccctcatgac gactgtcgtc ggctaactgg ctgcgcgcat gggtcacatc
    11161 cagcgacact gcgccccagt tcccgaggtt gcgccccagc cctaataaaa aggcggtgta
    11221 attggcagat aattgcgtcc cgccgtaggc agtaaattcc tgtggtaaac cgccgagtgc
    11281 cgtgccctga aaaaagaaag gcgatgattg ctgactatta ccgctacgaa aatcgcccgc
    11341 cgtcaggtca aatttgaaac gcccttcgcg ttgtaaaatt ggcactgttg aatacggaat
    11401 tgtgtaattc tgctgattac catcgcgctc gtcgatggtg acgtccagat cgccatttga
    11461 agatgtcggg tgcaaatctg taatttcaaa agcgccgggg gaaacatagc tttgatagat
    11521 aataaaacca ttttgccgaa tcgttagctg ggccgccgta cgggcaatcc cacgtaccgt
    11581 tggggcaaac ccttgctggc tatcaggata catattatcg gaagaataaa gccgtacacc
    11641 acgaaatcca acaccatcga aaatatcact tcctgtattg ccgtctccca ttaccagttc
    11701 acttttcagc ggaataatgg cgcgctgtac ccaggtgcca atattattcc actgttctga
    11761 atgatatcca tttccgcgaa aatagttcca ggaaccattg ttgcgtaaac gccacgggcc
    11821 aatattaatc ccgctgagct cactaaaaaa ataactatcg ttaccattac ctctgttacc
    11881 agtgaaatta taattcagga gtaaagcagg aataccttca tcccactctt caggcggaat
    11941 gtaaccgtga gcgctactca acaacgctat ctgaggaata gtgatgttca ggcgcatcgc
    12001 tgcaaaatca aagttaattg tggcatcagg tacagctttc agtaaattga tgcatttatt
    12061 gttttgctgc tgggctaact cgggaaatgc actgctatta aggccaattc gttcaaggag
    12121 tacctggtta aaacagggca ttaacccacc tgatttatca cctgtatttt ctgttgtcga
    12181 ttcaaatacg atatcctgtg aaccaatgaa ctcatcatta cgccagagat ctacccgata
    12241 cacgcccgca ggttgatgat tacctctctc aaagcgcgat aaatcagcaa cggatgtgcc
    12301 attctctaat aaaaatgccg ggttgaaata actttccgca tagcttaaaa aggagggtaa
    12361 aatggcaaga gatgaataac aaattccggc cagacaccag gtataccttt gcggaatatc
    12421 cgtagtagtg ggtattttca taattagctc tgatattgtc agatgattcg gttagtttaa
    12481 gttcacttct ctgaccgggg ttactgaacc ataatcatta acggtctgaa atttcagctt
    12541 tccctgcact cctgagggta agggaatttg tgaattaact ctgggtgcag ccatagcatt
    12601 ccccaacttt tggctgccca cttgtaagtt aaccagggtg acataaaaag gtgatggatt
    12661 atgaacattg atatagttac cggatcgcga aaacttgagt gtgtccggcg cttctgcggg
    12721 taattcttgt aattgaattg gacggagaaa taatttcatg cgcgataaaa tcgccagttg
    12781 caaaacattc ctgccgttca atgcattttt atctaccgaa gggatcgtct taacattcat
    12841 ccagaacaga gactcacgat ctgctgccag cggtggaccg gtgtaaataa tacgcaaagt
    12901 attttcgctt ttgggttcac taacaaatag tggcggtgta atgatgaatg acttttcttt
    12961 tacaccgctg ctgttttcaa tccacgaatt gaccagaaaa cgctcattgg tatggctatt
    13021 tctaatccat accgcagtct gtttagcatc agcgggataa ataatacgcg tggcacctaa
    13081 tgcgatccct ccggcagcat gggcgctggc cgaaatgatt aaataaaata taatgagcat
    13141 caataacttt attttagtca tcatagttaa tacgccttgt gtattcctga cggcaaaaat
    13201 aagaaatata aagaaatgat tgctttggtt tcttacaaca catcattccc ttttcattta
    13261 ataaagcggc atgatacaaa gtgaacatga tttcgattta cattgccacc ataccgcttc
    13321 agacaaccgg tacattactt tgttataaga cgtgatatat aattcaaaac aacgtggttt
    13381 tgattattcg tatctcataa taaaagtcgc gtcagcattt gcttgccctg ctgatgcact
    13441 tgtaccggtg cctttataac gtgcagaaaa atgaagaacg ttggtcccgg ggatcaagtt
    13501 ctggttggtt gagaagctat tcccatcagg tttgagaatt gcggatgtat tatcaagtat
    13561 ttcaataccg acacccgttg ctgttgttgt attggtactg gatgcaatgg ccaataaatt
    13621 atcattgatt gcatcagcct gaccagaaaa tgcaacggca gcattagcgg caacaacagg
    13681 atcgcagtcg ttcaactgta tagtgaatgg aattaatgca gaggtattac caacagcatt
    13741 gaaaatatcg gtacgatatt gaccgagtgt gacaacctgg tctgccgagt cagtattcac
    13801 tgaacaggca gcattcacca gttcgccttc aaagtgaatt gtaccgccat ctacaacggc
    13861 agcataacta cccgtagcgg tgaatagtgc ggcagccagc gcagacgaaa taaatcttaa
    13921 tttcatatat attccttcaa tctcatttat cgactccaca tccgtatata accgattact
    13981 ttatttaaga cactgatagt agtaaattcc tttttatcct ctaagaatgt cttaattgaa
    14041 aatatgcact ctattctaaa aaatagagag ccccgttaga tgaatacttc cgcgca
//
LOCUS       DAZGCQ010000123        14031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000123 DAZGCQ010000000
VERSION     DAZGCQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.123"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            242..541
                     /gene="yoaC"
                     /locus_tag="ACHK07_004178"
     CDS             242..541
                     /gene="yoaC"
                     /locus_tag="ACHK07_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO8104439.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHK07_004179"
     CDS             complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHK07_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO8104440.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            914..2275
                     /gene="pabB"
                     /locus_tag="ACHK07_004180"
     CDS             914..2275
                     /gene="pabB"
                     /locus_tag="ACHK07_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO8104441.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2279..2857
                     /gene="nudL"
                     /locus_tag="ACHK07_004181"
     CDS             2279..2857
                     /gene="nudL"
                     /locus_tag="ACHK07_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO8104442.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHK07_004182"
     CDS             3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHK07_004182"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8104443.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHK07_004183"
     CDS             4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHK07_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8104444.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHK07_004184"
     CDS             complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHK07_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO8104445.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHK07_004185"
     CDS             complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHK07_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO8104446.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8157..9128
                     /gene="manX"
                     /locus_tag="ACHK07_004186"
     CDS             8157..9128
                     /gene="manX"
                     /locus_tag="ACHK07_004186"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO8104447.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9191..9991
                     /gene="manY"
                     /locus_tag="ACHK07_004187"
     CDS             9191..9991
                     /gene="manY"
                     /locus_tag="ACHK07_004187"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO8104448.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10004..10855
                     /gene="manZ"
                     /locus_tag="ACHK07_004188"
     CDS             10004..10855
                     /gene="manZ"
                     /locus_tag="ACHK07_004188"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO8104449.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10910..11368
                     /gene="yobD"
                     /locus_tag="ACHK07_004189"
     CDS             10910..11368
                     /gene="yobD"
                     /locus_tag="ACHK07_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO8104450.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11679..11801
                     /locus_tag="ACHK07_004190"
     CDS             11679..11801
                     /locus_tag="ACHK07_004190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104451.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            11798..12364
                     /gene="mntP"
                     /locus_tag="ACHK07_004191"
     CDS             11798..12364
                     /gene="mntP"
                     /locus_tag="ACHK07_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO8104452.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWPHFHG"
     gene            complement(12361..13170)
                     /gene="rlmA"
                     /locus_tag="ACHK07_004192"
     CDS             complement(12361..13170)
                     /gene="rlmA"
                     /locus_tag="ACHK07_004192"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO8104453.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13336..13545)
                     /gene="cspE"
                     /locus_tag="ACHK07_004193"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13336..13545)
                     /gene="cspE"
                     /locus_tag="ACHK07_004193"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8104454.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13558..13701)
                     /gene="yobF"
                     /locus_tag="ACHK07_004194"
     CDS             complement(13558..13701)
                     /gene="yobF"
                     /locus_tag="ACHK07_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO8104455.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3474 a         3500 c         3476 g         3581 t
ORIGIN      
        1 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
       61 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcaca gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      541 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      601 ctttttctca caatcaaata atcatcggct tctataacga aatcgatacg gattagtgtg
      661 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      721 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      781 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      841 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      901 agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga
      961 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1021 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1081 tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1141 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1201 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1261 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1321 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1381 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga
     1441 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1501 atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1561 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1621 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1681 gccagagcgg tttatccttt gtgataatag tgaaatccag acccgaccga ttaaaggcac
     1741 gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1801 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1861 tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc
     1921 cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga
     1981 tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat
     2041 ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta
     2101 tttgagcttt tgcggcaaca tggataccag cattactatc cgcactctga ctgccattaa
     2161 cggacaaata tactgctctg cgggcggtgg aattgtcgcc gatagccagg aagaagcgga
     2221 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2281 ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca
     2341 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg
     2401 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2461 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2521 gcgcgaagct gaagaagagg ttgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2581 gccgcccgtc gatagcgtaa ctggctacca ggtaacccca gtggtcggca ttatcccgcc
     2641 cgatctgccg tatcgcgcca gtgaagatga agtctcggcg gtgtttgaaa tgccgctcgc
     2701 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg gcgattcaca
     2761 tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2821 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2881 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     2941 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3001 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3061 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcaggtaa
     3121 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt
     3181 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3241 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3301 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt
     3361 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3421 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3481 cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg aagtgagcgt
     3541 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3601 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3661 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3721 aggtgtactg ccaggcccgc tgcgcgtgcc gcgtcgtgcg tctgccctgc gccggatgct
     3781 ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3841 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3901 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     3961 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4021 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4081 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4141 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4201 ggttcaggtg ccgtgcattg agcgtaatgc cattgcatct gtgaaggcga ttaacgccgc
     4261 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac
     4321 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4381 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4441 atttatcccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4501 ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta
     4561 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4621 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4681 tcgccaatca tgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg
     4741 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4801 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactctatt
     4861 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4921 caccgcaacc acttttacgc ctgacgatcg accgtgccct gattaaaggc agtccggttt
     4981 tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag atgattaaca
     5041 tcgacttact gacggcaatg ctccttgagc cacaactgcc gcaaatcagt agcgccagcc
     5101 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccgcaac
     5161 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta
     5221 atggtccggg ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5281 tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct
     5341 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5401 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc
     5461 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5521 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5581 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5641 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5701 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctgcatc
     5761 gtaaaaatgt caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc
     5821 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5881 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     5941 ttgaactggt ggcggagggc gtggaaacgc aagaacaagc gaagtatttg cgccgtcatg
     6001 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6061 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6121 tgccattacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6181 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6241 atcacatccc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6301 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6361 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6421 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac caagccttgt
     6481 accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc
     6541 agcaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc
     6601 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg
     6661 atgatttcat ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc
     6721 tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6781 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6841 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6901 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     6961 cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact
     7021 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7081 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7141 accgtggggt gctggttaac aaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7201 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tacagcagta
     7261 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gactacccag
     7321 aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc
     7381 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7441 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7501 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct
     7561 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7621 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7681 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7741 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt
     7801 taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat
     7861 actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7921 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     7981 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8041 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8101 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8161 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8221 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8281 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8341 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8401 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8461 taatggcccg tgatgatgac ccaagctttg atgaactggt tgcgctggca gtagaaacag
     8521 gccgtgaagg cgtgaaagcg ctgaaagcca aaccggttga aaaagccgcg ccagcacccg
     8581 ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8641 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8701 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8761 ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8821 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc
     8881 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgta aaaatcacct
     8941 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt
     9001 cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt attgagctgg
     9061 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9121 ataagtaacg tattgtgttg attatcactc agttttcaca ctttagtctt acgtaaacag
     9181 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9241 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9301 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9361 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9421 atctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtattgc
     9481 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9541 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9601 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9661 cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9721 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9781 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9841 agcattcacc aactttaacc tggttgctct gggtgtgatc ggtactgtta tggcagtgct
     9901 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggctgctgg
     9961 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10021 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
    10081 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10141 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10201 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10261 cctagcgctg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg ctatcaacgg
    10321 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10381 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10441 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10501 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10561 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10621 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10681 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10741 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10801 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10861 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10921 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    10981 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11041 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11101 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11161 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11221 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11281 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaaaaat
    11341 ttataagctt ctcgtatcaa ctcaataact tatgaattta gccaaagcta tgtttagtgt
    11401 atttttaata atcagacata gcttaggcta tattatctct tcccttattt gttatttata
    11461 ttaacgtttc attgatatat aaatctaaat gaaaatcgtt atcaataaag caatgaaata
    11521 atatattcca acagttgttt tatattctca aaatatgtta aggttgcgcc ctcatttggg
    11581 gagtagccga tttccagatt ccggaaatgt acgtgtcaac atactcgttg caaaacgtgg
    11641 cacgtacgga ctgaagactt tcagtcaggc gagaccatat gcacatcaat cgctatgcct
    11701 gcatgaacgc gaaaacccat cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga
    11761 tgatgtgttt tatggatacc ccggtcagga cgttgtcatg aatatcactg ctactgttct
    11821 tcttgcgttt ggtatgtcga tggatgcatt tgctgcatca atcggtaaag gtgccaccct
    11881 ccataaaccg aaattttctg aagcattgcg aaccggcctt atttttggtg ccgtcgaaac
    11941 cctgacgccg ctgatcggct ggggaatggg catgttagcc agccggtttg tccttgaatg
    12001 gaatcactgg attgcgtttg tgctgctgat attcctcggc gggcgaatga ttattgaggg
    12061 ttttcgtggc gcagatgatg aagatgaaga gccgcgccgt cggcacggtt tctggctact
    12121 ggtaaccacc gcgattgcca ccagcctgga tgccatggct gtgggtgttg gtcttgcttt
    12181 ccttcaggtc aacattatcg cgaccgcatt ggccattggt tgtgcaacct tgattatgtc
    12241 gacattaggg atgatggttg gtcgctttat cggctcaatt attgggaaaa aagcggaaat
    12301 tctcggcggg ctggtgctga tcggcatcgg cgtccagatc ctctggccgc acttccacgg
    12361 ttaataagaa cgctgccaga ggtgaatatt aaagtccgtc tggcagtcga acacttcttt
    12421 tgctgccagc gtttgccaga cttctggctt cgcacgccag gcaaacggcg tcatctgcaa
    12481 taatgcgacg gcttcatcac cgcgaagacg catcggataa cacaactcat cactctgctg
    12541 taatgtaaaa ccttccagtt gttctgcatg aggtgcatga agatgtactt cattgtaaat
    12601 cagccccttc agctccatca aatgtcgcgg tcccggcgtg gcagtaatga cccaaccgcc
    12661 gggcttcact actcgcgcta attcttctgc tttacacggc gcgtaaatac gtattatggc
    12721 gtccatactg gtatcggaaa acggcaaacg gtggctggaa gcgacacaaa aagtgacctg
    12781 cggatagcgt ttcgccgccg cttttatcgc taccttcgag acatccaggc caaaggtggt
    12841 gatttcgggc aacgcatcgg caaatgcgtg tgtgtaatac ccttcaccac agccaatatc
    12901 cagcaccgcc gtggccttat catcaagccg ttcccgcagt tgggcgacaa ttgcatcacg
    12961 cagcggctga taatgtccgg catctaagaa tgcgcggcgt gcttgcatca tttccgcgct
    13021 gtcgcccgga tcacgagacc gtttatgctg aacgggcagc agattgacat acccttcttt
    13081 cgccatatca aactgatgtc gttggggaca gatatagctg tttttttcac gcgtaagagg
    13141 ctgatggcaa agtggacagg aaaacgacat gacaactccg gcaggatact aaaggccgca
    13201 agtgtaacgc gaatcacgcg gcgggcaaat atccgaacgc caggagcgaa gataaagcat
    13261 agtaaaaagc ctcgcattcg acgaggcttt atagcgagat tgaagcgtat tcacacttca
    13321 gatcagtgga ttcgatcaga tagctgttac gttaacagct gccggacctt tctggccgtc
    13381 ctgaatttcg aactcaacgt tctgaccttc agccagagtt ttgaagccat taccctggat
    13441 agcggagaag tgtacgaaca cgtctttgct gccatcagca ggagtaatga agccaaaacc
    13501 tttagactcg ttgaaccact taacctgacc tttaatcttt gccatttgaa aaattcctta
    13561 gattgttttc ttcgcccgca ggcataacat agataaaact gacacattac tgcatgaggc
    13621 accaatataa ggctcggcag agaagcggta ttcaacgtca acgtgtttac tcaggacttc
    13681 tttactgaaa atgccacaca taaacagaac tgtacctcgt ttaacccgaa atctgttatc
    13741 acatacaacg ttaattatgg caagccattt ttaaacatgt ctcgatcaga cgcacaaatc
    13801 tcgatacgtc ttccactttt tttgcacact tatgcaacgg aatacgcgcc gatatatcat
    13861 tgtgcttaac cttgccagtt caggcagata cttaaactgg cgtagtttct aacatagttc
    13921 aataagttca gttgttccag gccagtggga gaagttatta catagtgcgt gcaatatcac
    13981 attttctgct atgcaatgaa taaaaagtta tatcactttt tctcataaaa c
//
LOCUS       DAZGCQ010000124        13993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000124 DAZGCQ010000000
VERSION     DAZGCQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.124"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..1284
                     /gene="citC"
                     /locus_tag="ACHK07_004195"
     CDS             226..1284
                     /gene="citC"
                     /locus_tag="ACHK07_004195"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO8104456.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1299..1595
                     /gene="citD"
                     /locus_tag="ACHK07_004196"
     CDS             1299..1595
                     /gene="citD"
                     /locus_tag="ACHK07_004196"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO8104457.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1592..2500
                     /gene="citE"
                     /locus_tag="ACHK07_004197"
     CDS             1592..2500
                     /gene="citE"
                     /locus_tag="ACHK07_004197"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO8104458.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2511..4043
                     /gene="citF"
                     /locus_tag="ACHK07_004198"
     CDS             2511..4043
                     /gene="citF"
                     /locus_tag="ACHK07_004198"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO8104459.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4047..4598
                     /gene="citX"
                     /locus_tag="ACHK07_004199"
     CDS             4047..4598
                     /gene="citX"
                     /locus_tag="ACHK07_004199"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO8104460.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4573..5451
                     /gene="citG"
                     /locus_tag="ACHK07_004200"
     CDS             4573..5451
                     /gene="citG"
                     /locus_tag="ACHK07_004200"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO8104461.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5502..6965
                     /gene="citT"
                     /locus_tag="ACHK07_004201"
     CDS             5502..6965
                     /gene="citT"
                     /locus_tag="ACHK07_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO8104462.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7079..7885
                     /gene="rna"
                     /locus_tag="ACHK07_004202"
     CDS             7079..7885
                     /gene="rna"
                     /locus_tag="ACHK07_004202"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO8104463.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEMQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8115..8525
                     /gene="rnk"
                     /locus_tag="ACHK07_004203"
     CDS             8115..8525
                     /gene="rnk"
                     /locus_tag="ACHK07_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO8104464.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8663..8752
                     /gene="yldA"
                     /locus_tag="ACHK07_004204"
     CDS             8663..8752
                     /gene="yldA"
                     /locus_tag="ACHK07_004204"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO8104465.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8756..9994)
                     /gene="ybdR"
                     /locus_tag="ACHK07_004205"
     CDS             complement(8756..9994)
                     /gene="ybdR"
                     /locus_tag="ACHK07_004205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO8104466.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10214..10642
                     /gene="uspG"
                     /locus_tag="ACHK07_004206"
     CDS             10214..10642
                     /gene="uspG"
                     /locus_tag="ACHK07_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO8104467.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10763..12328)
                     /gene="ahpF"
                     /locus_tag="ACHK07_004207"
     CDS             complement(10763..12328)
                     /gene="ahpF"
                     /locus_tag="ACHK07_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO8104468.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12457..13020)
                     /gene="ahpC"
                     /locus_tag="ACHK07_004208"
     CDS             complement(12457..13020)
                     /gene="ahpC"
                     /locus_tag="ACHK07_004208"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO8104469.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13392..>13993
                     /gene="dsbG"
                     /locus_tag="ACHK07_004209"
     CDS             13392..>13993
                     /gene="dsbG"
                     /locus_tag="ACHK07_004209"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO8104470.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLKVPANVSTEQMKV"
BASE COUNT         3313 a         3725 c         3520 g         3435 t
ORIGIN      
        1 aaaaaactta aaaccattaa ataaataaaa aaccactaac tctatgtgaa ataaatcaaa
       61 atttcacgcc aaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
      121 cacccccctt tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt
      181 cgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat
      241 attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      301 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      361 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      421 gaatccgttc gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc
      481 tatgagcggc acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc
      541 cgccagtgcg gtttttccac gttgaccagc gtaccaggcg tgatggtgct gatggaaaac
      601 agcgccacgc gattgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tcccgggaac
      661 aagattggct gcattgtgat gaacgccaat ccctttacga agggtcaccg ttatctgatt
      721 caacaggctg cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca
      781 cgcttcccct atgaagaccg actggatctg gtgttaaaag gcaccgccga tattccacgc
      841 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      901 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatttgaa gattttccgt
      961 cagtacctcg ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt
     1021 cgcgttaccg cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc
     1081 gcaccgccca tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc
     1141 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1201 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1261 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1321 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1381 atatcgacct gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1441 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1501 aaggcgcact ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg
     1561 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1621 aactcgcacc cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa
     1681 ctccttcatc tacccggctg atgccctgat gtttgacctc gaagactccg tagcattgcg
     1741 tgaaaaagac accgcccgcc gcatggttta ccacgcgctg caacatccgc tgtatcgcga
     1801 tattgaaacc attgtgcgtg tcaacgcgct ggattccgaa tggggtgtca acgacctgga
     1861 agccgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1921 tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1981 cagcaccggc ctgctggcgg cgattgaatc tccgctgggg attacccgcg cagtggaaat
     2041 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2101 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt
     2161 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2221 cgaagccgga ttcctgcaag aagccgccca catcaagcag ctgggctttg acggcaaatc
     2281 gctgatcaac ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga
     2341 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2401 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2461 gctctcccgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2521 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2581 atcttgccgc tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2641 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgatc aggtttacag gacggcatga
     2701 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2761 tcatcgcgaa gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc
     2821 atgcgccgct ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc
     2881 tgcgcggtcc actggcagaa gaaatttccc gtggtctgct tgcggaaccg gtgcaaatcc
     2941 actctcacgg cggtcgcgtg catctggtac agagtggcga actgaatatc gacgtggctt
     3001 tcctcggcgt cccgtcctgc gatgaattcg gtaatgccaa cggctacacc ggtaaagcct
     3061 gctgcggctc cctcggctat gcaatggtcg atgccgacaa cgcaaaacag gtcgtgatgc
     3121 tcaccgaaga actgctgcct tatccgcata atccggcaag cattgaacaa gatcaggttg
     3181 atctgatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgcgaccc
     3241 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca
     3301 actctggcta cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg
     3361 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3421 ttggcggtat taccgcgacg atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc
     3481 tggatgtgca gagctttgac agccatgctg cgcagtcgct ggcccgtaac cccaatcaca
     3541 tcgaaatcag cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc
     3601 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3661 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcaattgcct
     3721 ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata
     3781 acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta
     3841 tcgcagttaa cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag
     3901 tggtttccat tgagtggctg cgcgaacgcg cgcgtctgct gaccggtgaa ccacagccga
     3961 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     4021 ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4081 cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct
     4141 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4201 aagacagcga ggtcacacgc cgaattttta atcatggcgt gaccgccttg tgtgccttag
     4261 ccgcaaaaca gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4321 agggcatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4381 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa
     4441 ttctctcccg ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gcgcaaagcg
     4501 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4561 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4621 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4681 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc ataaagatat ggcgctggaa
     4741 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4801 gcctgtagtg cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg
     4861 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4921 ttttctttag ggctgctatg tgcggcaatt ggacgtttgc ttcagctcaa ccagccggtt
     4981 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     5041 ctgcgtacca ataattcaca actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5101 accggcgcac gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg
     5161 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5221 ctcctactga tggcgatcaa cggcgatacc aacgttgcgt cgcgcggtgg cgaggggggc
     5281 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5341 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5401 ggcggcagtg ccgacctgct gatccttacc tggtttttag cacagattta attatttaag
     5461 cacttgataa attttgaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5521 atatatggaa actattggcc ccactggtgg tgatgggcgt catgtttctt atccctgtcc
     5581 ccgacggtat gccgccgcag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5641 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5701 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5761 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5821 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5881 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5941 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     6001 tttttccggt cattaaaaac ctgccgccgc tgtttaaatc attcccgaac gatccgtccg
     6061 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6121 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6181 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6241 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6301 aagaagtggc aacctgggcg ggtgatgaac tgaaaaccat gggtgcgctg acacgcagag
     6361 agtggacact gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag
     6421 tcattaatgc tactgcggta ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg
     6481 tgccgtggaa agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6541 ctctggttgt gatggctaac ggcctgactc gttctggctt tattgactgg ttcgccggta
     6601 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6661 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6721 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6781 tgctggtgct gtctattggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6841 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6901 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6961 actaatacgt tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc
     7021 gggctttttt atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat
     7081 gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7141 caacgccgta gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7201 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7261 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7321 gccaggattg cctaaatcgg tcgctgcccg tggtgttgat gaacgccgct ggatgcgctt
     7381 cggttgcgct actcgcccaa tcccgaatct accagaagcg cgcgccagcc gaatgtgttc
     7441 atcgccggaa accgggttat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg
     7501 agctggcgga cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg
     7561 ttttgatccg gacgcatact tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag
     7621 cgaagccgga aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga
     7681 cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca
     7741 gggtaaccct gcgtatttga ctgaaatgca gatctcgatc aaagctgacg ccatcaacgc
     7801 tccgctttct gccaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt
     7861 tgtgattgat aaagcgggtt attaatcctc tgatttacaa aacacgctaa ttcgtcttcc
     7921 ataaaccaag gcgattaaga tttatacata atgcgctgca ttgaggaatt ccctgaatgt
     7981 gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg
     8041 ccgcctgacg gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac
     8101 gacatggagt aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca
     8161 tcgatattct gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg
     8221 cagagttgga tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa
     8281 tgaacagccg ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg
     8341 tgtatccggc aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg
     8401 ccgcactgct ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg
     8461 caacccacct tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc
     8521 tttaatccca tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct
     8581 ttacgcaaaa tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc
     8641 cgtttcgttt gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat
     8701 tatggcggcg attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgatcata
     8761 ttgttccccc cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc
     8821 tttgtgcacc gggtaccaga atcaccttac ggcactcctc ttcacgtttt tcgaaaatct
     8881 catatccccg ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca
     8941 gtaatccttt ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc
     9001 ccattttaaa cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc
     9061 cagcgtagac gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc
     9121 gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc
     9181 tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg
     9241 tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc
     9301 cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt
     9361 ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cacaggaccg gcaccataga
     9421 ctgcaacgct tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca
     9481 gaatatcaga aaggaaaagc gctttatcat ctgaaagcaa aggcggtact ttaaacggcc
     9541 ccacattccc tttagggacg cggacatatt ccgcctgccc gccaggaacg ccaccataca
     9601 ggtgactata accaaacaat gccgcgggag caggtatctg ttttttattg agcgcagcgc
     9661 ctttacccgc attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt
     9721 cgccacaagc aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt
     9781 ccttcccggt ttcaactact tcccccataa attcatgacc aaaaatatcg ccatgtttaa
     9841 cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa
     9901 tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa
     9961 cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa
    10021 agtcagtctt taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga
    10081 gaattcctgg tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa
    10141 ccagacaaat atcagggata aatattataa tcatggcgaa tgatttgttc atgattaaca
    10201 gggagaaagg gttatgtata agacaatcat tatgccagtt gatgtatttg aaatggaatt
    10261 gagcgacaaa gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct
    10321 acttcacgta ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg
    10381 tcgttttgaa gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca
    10441 cttcaccatc gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga
    10501 agtcaatgag ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc
    10561 atcgatttcg acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct
    10621 gcctgtactg gtcgttcgct aacagataaa aatatttatg ccgccctgcc tggaacatcc
    10681 cggcagggct ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat
    10741 tgcaggtgaa tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac
    10801 tcagagaggc tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg
    10861 tacagtcacc cgctgcgaac acgcctttga cgttggtttc gcatttcgca tcaatgataa
    10921 tctcgcccat gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac
    10981 caatctggac aaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc
    11041 gatattccag accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca
    11101 gaataatgtc gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca
    11161 tttctggcgc aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg
    11221 cttccacgcc ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcgggc
    11281 cgtcgcagtg cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt
    11341 tcatgttgcg ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc
    11401 cagaagctgt ttcaatctgg tgtagaccac cttcaactgc tgccgggatc agtttgctgg
    11461 cgctctggct gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agcgcacctg
    11521 ccagtttctg gccttcggtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga
    11581 ggatctgacc accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt
    11641 aaattgctgc cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac
    11701 gcttgttcag ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt
    11761 cagtcaacgt catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc
    11821 ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct
    11881 tgatgcgcgg gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat
    11941 ggcaagagag cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct
    12001 ccagcagaga ctgcgcttct ttcgacggat gaccaccggt ccacagtaac gccagtacca
    12061 gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttagaacctg
    12121 ggttggtgat caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga
    12181 ctttgtctga cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc
    12241 tgtcatccag cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga
    12301 gttgagtttt catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc
    12361 aagcggcctc tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg
    12421 tgccgcagca cccgattaat tacgcgattg cggaacttag attttaccaa ccaggtccag
    12481 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    12541 agaagctacg tactgtgctg ctttgatttt acgcagcagg tcagacgcgt cacggccaat
    12601 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    12661 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    12721 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    12781 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    12841 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    12901 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    12961 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    13021 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttacc gattcggcta
    13081 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    13141 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    13201 aatctgaact accctttggt atcttcagcg cctgtctgca ggctgcggtg ttgctcttac
    13261 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    13321 agctcttacc tatgacaatg ataggttatg ccttttactc gacttttgca ctgactgaaa
    13381 aggacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    13441 cagaggaact tcctgctcca gtaaaagcga ttgaaaaaca gggtattacg atcatcaaaa
    13501 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    13561 ccatctacct gactccagat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    13621 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    13681 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagac gcgccggtca
    13741 ttgtctacgt cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    13801 gcccgtgggt agattctggc aaagtacagt tgagaacatt gttggttggg gttatcaaac
    13861 cagaaagtcc ggcgacagcc gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    13921 aagaatatga agcctctggt ggcaagctta agctaaaggt gcctgcaaac gtaagtacag
    13981 agcaaatgaa agt
//
LOCUS       DAZGCQ010000125        13745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000125 DAZGCQ010000000
VERSION     DAZGCQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.125"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK07_004210"
     CDS             complement(<1..275)
                     /locus_tag="ACHK07_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8104471.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
     gene            complement(485..1639)
                     /gene="ompC"
                     /locus_tag="ACHK07_004211"
     CDS             complement(485..1639)
                     /gene="ompC"
                     /locus_tag="ACHK07_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8104472.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            complement(1838..2179)
                     /locus_tag="ACHK07_004212"
     CDS             complement(1838..2179)
                     /locus_tag="ACHK07_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8104473.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(2172..5414)
                     /locus_tag="ACHK07_004213"
     CDS             complement(2172..5414)
                     /locus_tag="ACHK07_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8104474.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLSNP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(5478..5891)
                     /locus_tag="ACHK07_004214"
     CDS             complement(5478..5891)
                     /locus_tag="ACHK07_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104475.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(5955..6164)
                     /locus_tag="ACHK07_004215"
     CDS             complement(5955..6164)
                     /locus_tag="ACHK07_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO8104476.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(6260..6634)
                     /locus_tag="ACHK07_004216"
     CDS             complement(6260..6634)
                     /locus_tag="ACHK07_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8104477.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(6649..7365)
                     /locus_tag="ACHK07_004217"
     CDS             complement(6649..7365)
                     /locus_tag="ACHK07_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO8104478.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(7431..7775)
                     /locus_tag="ACHK07_004218"
     CDS             complement(7431..7775)
                     /locus_tag="ACHK07_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO8104479.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(7772..8218)
                     /locus_tag="ACHK07_004219"
     CDS             complement(7772..8218)
                     /locus_tag="ACHK07_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO8104480.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGIESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(8215..8565)
                     /locus_tag="ACHK07_004220"
     CDS             complement(8215..8565)
                     /locus_tag="ACHK07_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8104481.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(8575..8901)
                     /locus_tag="ACHK07_004221"
     CDS             complement(8575..8901)
                     /locus_tag="ACHK07_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8104482.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(8898..11320)
                     /locus_tag="ACHK07_004222"
                     /pseudo
     CDS             complement(8898..11320)
                     /locus_tag="ACHK07_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(11266..11487)
                     /locus_tag="ACHK07_004223"
     CDS             complement(11266..11487)
                     /locus_tag="ACHK07_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104483.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVQRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(11532..13469)
                     /locus_tag="ACHK07_004224"
     CDS             complement(11532..13469)
                     /locus_tag="ACHK07_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8104484.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(13533..>13745)
                     /locus_tag="ACHK07_004225"
     CDS             complement(13533..>13745)
                     /locus_tag="ACHK07_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8104485.1"
                     /translation="AEGVLVHGGQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPL
                     MALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT         3024 a         4364 c         3188 g         3169 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ccggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcaat ttcccagagc accacccgtg gtgactgctc tgacttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatatatc cgcccataaa aaaggggcgc
      301 agacgcgccc cgaacaataa aagtccaatt taaaataaaa ccaataaagg taataacaga
      361 aagttaaatc agcacatgcc agatggcaat tgctgatcgc agtgaccaat gaaaactgtg
      421 ttttcattga tacaggccgc ctgtaagggc agacggcccg tatttattaa cttatcagca
      481 ataatcagaa ctgataagtc atacccacag caacgatatt atctgtcgca acaccggctt
      541 tccgggtaaa ttcactttca tcaatcaggt taattttata atcaacatag gtggacatat
      601 tcttgttaaa gtaataagtc gcaccgacat caatatattt aaccagatcc tgatcgccat
      661 ggttcacacc accaaccata acatcctgtc cgcgggactg aaggtaggca agggacgggc
      721 gcagaccaaa atcaaactga tactgaacaa cggcttcgat attctgagct ttatccgcaa
      781 caccttgatc accaaaggtc gtcatattct gggtttcaga atacactgct gccagataca
      841 ggttattggc atcatattta agaccagttc cccagaattc agcatgttta ccttttgcca
      901 cactacctga gtcaactgtt ttttctccaa cctttttacc tgattcagga tcaagaacat
      961 ctgcagtacc gttcaacccc tgaacctgct tatcggtacg atctgacttg gtatacgcag
     1021 cgacaaaacc aaaaccatca aactcatagc tggccgagaa accatacccg tcgccattag
     1081 ccttctgaag gtcatcacgc tcatttttgc cctgatactg cgccgcaaaa ttcagaccat
     1141 caaccagtcc gaagaagtct gtattacggt aagtcaggac accggaagtt cgggccgtca
     1201 taaagttatc ggtctgcgtc cagccatcac caccaaactc aggcagtacg tcagtatatg
     1261 atccgacatc gtaagcaata ccatagttac gtccgtaatc aatggtacca acatctgcaa
     1321 atctcagacc tgcaaatgcc agacgggttt tatttcccgc ggaaccttca gattccgttt
     1381 tgttaccaga gaactggtat tcccactgac caaatccggt cagttgatca ttaatctgcg
     1441 tttcgccttt aaagccgaga cgagcataag tctggtctcc gtcattacct ttatcatccg
     1501 agaagtaatg cagagcggtt gcacgcccgt aaagatccag tttattacca tctttattat
     1561 aaacttctgc tgcctgcgcc cccgcagcaa acatcactgc cactgctaca gcagaaagtg
     1621 ccactgtcat ttttttcatg atttaatcct tatttaaact gaactattca tgcatttaga
     1681 tgtcatgaac aaaaattaaa atattttaat acaaattctt agttcaattt atattatgta
     1741 acaaaatgta aatacaagat cgatttcgca ctttctgaca agacagatta aaaataatta
     1801 acaactaaac aataaagagt gatttatagg ataattctca tctgactacc tgctctatcg
     1861 tgcaactgaa atcactgtac cgggcgttat ccgtaatact ccactcccgg cacaccaccc
     1921 tgaccgtccg gttatgtttc gggggcttcc acaaaaaagc ccggtaacca ccatgccatg
     1981 acagaaacgc ggacagtgcc tcccgctcag catccgttgt cacccgaaaa accacctgaa
     2041 aggtcttcag ttgcgcattc agtcctgccg gacgacgttg ctgataaccg tcgccaaact
     2101 tcaccgtcac caccgacggt ttctccgtca cctgcatccc ttcccgggga caccagtgaa
     2161 gggtcttaat ctcatccact cagcattcct ccgtcacgac gcatggataa catcaccgcc
     2221 tgtacccgct ggtcaatcag ttgcacaagg gtgcccgccg cttccggccc tatctgcccg
     2281 ttagcgccgt cattctgaat ggcaatgtgg tacaccgggg aatacaccag acccgcactg
     2341 ccgttcatac tgcccaccgc acgcacgccg agtgaaccat ccgctgcccg ggtcaggggc
     2401 atgatggctt caggtccggc ttcccccatc agccctgccc cttttgcaaa ggcaaagtac
     2461 gtgggcgtgt ccacaatact gttactgtat gcactcaggt ttgccgaggt ataaacgccg
     2521 ccttttgcat tggccaccgc gccacccagc cagttgccta tactgccgaa aaatcctcct
     2581 gcaccggaca tactgtttgc ggccatctta atgccgttga caatggccgc attcataaga
     2641 acttttgata tttcctgcag gattgatgca gcccagctgc gccattccac tttatttccg
     2701 ttcagcatct ccgtgatgtt attcaccatc cctgagatac cgtctgtcgc aagctgtgct
     2761 gcctgtgaag cgtaatctga tgcgctgtcc acccagttac ttagcccttc ctgcagccct
     2821 ttctgccagt cagcacgctg cgcatccgat tcggcataaa aagcctcctg gtctttaagg
     2881 cgttcactca gatactgcgc attctgcgcc agcgcctgtc tgtaaaaatc ctcactgata
     2941 tcccctgtct gatactgaga ctgcaggtcc gcatccttct ggcggtagcc gtcacgaatc
     3001 tgctgcagct cccgcatccg ttcacggacc cgctcaccct gcccgtaccc cagcagctca
     3061 gcgtcatttg atgccctttc gtctgcatta tcatttttca gagcctcctc ccgggcccgt
     3121 aactgctccc ggattttctt ctggtcaatc agggcagcat tgcgcagcaa ctcctgcttc
     3181 tgtatctccg tcagggtttt cagctctccc tgcgaggtct ggtacttcag cttcgccagt
     3241 tctgtattct tccctgccag tgccagttgt tccttctgct gcttcagcag acgggaaaaa
     3301 ctgtcttccg ctttttccgt ctcagatttt ccggcccggg atttgggttt attcgcctca
     3361 ttattgcgcc aggcttccat cgaattacgg atgtaacgct gcctcgcctc ctgatacgta
     3421 tcccccacca gaccaaggtc atccgccgca taccccagcc gggcacgctc tttttcttcc
     3481 cctttcagcc gggacagggc cagttgacgc tctgtgttat tcagggcgcc ctgctgttta
     3541 tcatccagag tggcctgtgg cagtcgcagc ggtacactcg ccagtccctg acgctgttgc
     3601 agcagttcat tacccagccc cagcaggcgg ttgaattccg tatgctgacc gttcatgatc
     3661 agcagtgact gatacgcttt attctgctct gccgcctgct gacgaattaa cacaacacgg
     3721 cgctcttcca gtccggcaag cacatcctga atggactgcg ctttttcctg catctgtgcc
     3781 agacgggact gctcaacagc aagctgctct gttgcctgag caagcccttc cgttacagtt
     3841 ttcaccgatg tcaggtggtt tatcatgaag ccgttaccgg tcgtccagcc cggattagac
     3901 agcacatact gatacccggc gattttttcc tgcagggatt tcacccggct ggcctgctca
     3961 tcaatcagcc ggttctgctc tgtcagcgcc tcccgtgttc gctcttcatt atctgaggct
     4021 tcaggcagag acattgatgg cgttttatgc gcgatttcat caatcgtcag tgcatactgg
     4081 cgcgcagact ccctggcctg ttcctgattc tggtacagcg tgtaccatgc ggcagctccc
     4141 agcatcacca gtccgggtac gcctccaacc agtcccagcg caccgctcat cagacgtgag
     4201 cccaccgccg ttgtactgtt cagcgcattc tgggcggcgg ttctggcagc aatatttctg
     4261 ttcaggcgtt cctgtgtgac cgccagacgg gcctctgccg caatctgcat ctcagtcccg
     4321 cgggctgccg ccacagcctg ctgagcccgg tacacggctg cccttgcccg cgccgtggca
     4381 atctgcgttc cccttaactg ggcctcagcc agtgccactt cattacgtgc agcggtcaca
     4441 agtcctgccg tggcagacat cgctccggaa gccagattac caaagtaccg ggccaccccg
     4501 acagcaacca gcacacccgc agctgttgcc acggtatcaa tatttccggc cacaccgttc
     4561 agtgcgccag agagcgtttt cgtcgctccg ctggcctcat tcgcgccgcc cacccaggcc
     4621 ataaaggcgt tttccacctt cgtgatcccg tcagacaccg tttccggcat ggctgcatat
     4681 tcatcacgca atatccccag ctggctgatt aacgcgggaa cgactttatc cgccgtcagt
     4741 tgaccatcgt ccgccatcgc cttcagatcc ttacgggcca cgcccatgcc tgcagccagt
     4801 gcgcgaatga tccggtcccc actttcattg accgaattaa attcctcgcc gcgcaacacc
     4861 ccctgtgcca gcgcctggct gaactgggtg atcaccgaac ccgcctctgc cgcactggca
     4921 ccggaaattt tcagccctgt cgaaatggcc tccgtcacct tcagcacatc atcagcactg
     4981 taaccatatt cacgcatcga ggcagccgaa cgggcaaaca gggccgcatt atccgaaaat
     5041 gcggtgcctg tccgctggct gatatccatc agcactttct gtgatgacga aaattcatcc
     5101 gatgactgcg acgcctgttt cagacgggca ttcacggagc tccattcatc cgccagcgaa
     5161 atcaggtgtc cggtggcaaa ggcaccagca aacgcaccgg tcattccgac agccgaagcg
     5221 cggatttccg tcaactggct gttcagctca gccagagccc ggcgctgctc cctggctgcc
     5281 gcagcagcct gacgtccgcc attctgcagg gtccggtaat attcactgcc catacgggac
     5341 gcccgctgga tctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
     5401 cgtaacgtcg ccattcacct ttctccgggc gaaaaaaaac ctgccgcagc aggttttatt
     5461 catcagcaga ttattgatta agttttgggt accagcccgg gattcagact ttccggactc
     5521 agtgtataca taccgccatt gaatgggtcc acaagcaacc agccaacaat accaccaaag
     5581 acaaagttac caccaatgta ccatccatta gccgatgcct tgatcggcag cgttacgggc
     5641 tgataattct ctttactgaa tgtgatctga taactctttt tcccgaagta actgccgtca
     5701 gatttttcaa gtgttacatt ctgcggtgtt ttgcccgaag aaattgtttt ccctgactca
     5761 tctttaataa taaaatccgc accagacgga ttactgttta cctgtacagt ctgcgtttta
     5821 tctccgacaa tggttgcaca ccccgtcaga aaaaagctga cagagaaaat caacagcatg
     5881 aatttcttca taaaatggct ccgttcagtt aattttcccc gaaaatactg taataaagat
     5941 ctgcaaagtt aaaattaatt ttccgaagca aataacagag catcctccag agcggccaaa
     6001 ggatctgcct ccggcttatc ctcatcctcg ccccagcaga gcatggcgtc cttcagtgca
     6061 acattcatcc cctgtgcccc gaaaaccgct ttcacgatct gtgcattacg gatatccccg
     6121 cgctcatcac ccagcgggga taccctgtcg aactccatcc acatcatcgc ctcgctcgca
     6181 ctcaggctgt ggcgcagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga
     6241 aagcgaattt ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca
     6301 gtgcctgacg cagcaagcgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt
     6361 cgtccggagt gaacacccgc tgcaggtccg tatcacacag gacatcgcag aacagcgtca
     6421 catccgcttc caggttacgg cgggttttcg ccaccaccga cagggtatcg tcatcctctc
     6481 catcaccatt gagcacgtcc tgccacagat accaggcctc tgccgaaggc tcccgcagca
     6541 ccacgctgac attaccccat tccggcacct tcaccgtttt atgacgaaac cctgacagtc
     6601 tggccagcgc cagcgttttc agatcctttt tcatgatgac ccatcccctt atccggcggc
     6661 tgcgctcact gtcactgtac attcaacaga cgtcacactc tgtgctttct ctgccgaatc
     6721 agtcaccacg caggtatatt tccccgcatc agcggactgc gcacctggct tactgaaggt
     6781 gtctgtcgtc tgcccgtcaa ccggctgacc atccttcttc caggcgtatt tatacggcgg
     6841 cgttcccccg ttggcactga ctgacattgt cagcagcgca ccggtattca cggtaagtgt
     6901 cttatccaga tttttcacaa acgccagcgg taccacaaag gacaccggtt tgcctttcag
     6961 acgcagtgag aacgttgcag ccaccacgcc gttggtaccg gatgaccagg tgtgctggcg
     7021 cacttccgcc aggaatttaa agcccttacc ggacggaaac tgcaccttaa acgcatacaa
     7081 cgcgtcattg tcataagcat cacgcagggc gttctgggcc tgattcagat aaaaattacc
     7141 cgacatggaa atctcggacg acgcccccag accattgata ttctcctgct ctgtggagca
     7201 gagcgtggtc acatcaatat cctgtttctg accggcggtg aactggactt ccttgatggt
     7261 gcagtccagg cgcagatatt ccgccttatc catagtttca gcagtcgccg gggcagatga
     7321 aatcatcacc tgcgtcagct gtgagcgttc atacaaagca gacattctgc ctcctgataa
     7381 taaaaaaccc gcacgcggcg gggtatgggt tttgtagaaa aaagaaaaag tcacaccgtg
     7441 acctgaaact ccagggttgc acggtaacag cggttttccg gaatatagtc ctgcatttca
     7501 ctgacggatc ccggggccag cagcattatg gcttcacggg cgtcctgacg tatctgacgc
     7561 gcctgcgtca cagtcccggc ataaacgtct atctgcaccg ccactgagga ctccgcctgc
     7621 ccgcccatca cgtccgcaga caccgatgaa atcaggctga aaaccaccca cggaagcgcc
     7681 accgacggcc tgccatccag cagggggacc acatacgggt acacctgccc gccggcaaga
     7741 tgcgccagat gaggatacaa atccgcctcc gtcatcgtct cagtacctca tcaatggccc
     7801 ggttcatccg cgcaatcgcc acctgcgctg cctgttcact gcgcacatca aacgccgggc
     7861 gcacaaacgg gtgcggtggc atattcacgg tccccatttc cacaaaccgc cagtagaaag
     7921 cattccgcgg gttatctgcc ttcatggtgt tatcgctgtt accggtgtcc ggattaacac
     7981 cacggatatg gacaccggat tctatcccgc catcgcggga gcgccgggaa aggaccacca
     8041 cattgcggcg cagttttccc ctgcgcaccg gtgcccgtga caccacttct tctttcagca
     8101 cattcgcacc cgcacgggtt gcctcacgca gcacccggtt attttccgca ccactcagaa
     8161 gctgcaaatc gcggctgatg tcctccagcc ccgaaaaatc cagcagggtt tcgatcattt
     8221 ttcccctccc agccgacaga gaatttccag acgcccgccg gtcgcatccg gcacgggcag
     8281 cccgacaacg ttcaggatcc ggtcacgcca tggaccactc agcacatgaa gtcgtgacgc
     8341 tgccgtgatt tcccgaccgg actgaccgcg cacccagatg cggatttccg cctgcgccat
     8401 ttccgcaccg gactgcatcc gctcccggct gctcctgcca cggatatccg catgaatttt
     8461 cccgcatgac acccattctt ccgtcatttc tccggcagca ttacgggtta acaccgggtt
     8521 cagaacactt atcatctgtg tcagacgacc tgcagatatt gccattcccc ctcctcataa
     8581 caccgtcgga caacgcaaat cgtaaatcag cacggaaaca gaaaacggca gctccccctg
     8641 aagcagttct tcccgctccg caagatccgg attccggtac agcatcccgg tcagtcgcat
     8701 ggcagccccc ttcatccggg ttaatgcctc gcccgggatc agttcaccgt cctcacgaat
     8761 caccttatcc cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt
     8821 gtccatcagc atgtcatcat ccgcgtcatg gtcgacacgc agatgtgcct tgatctcttc
     8881 cagtgtcagt aatgccgtca ttttccacct cctgcatccc gcccacgttt tgcagccagg
     8941 gtccaggctg atgaatgagc ttctccgggt ttatcaccgg tcatactgtt gcagtgccac
     9001 agcgagcccc cccatgtcac cgtatcgccg gggtggtagg tttcaccggc tctgaacaca
     9061 ccgcggtaga gcatcaccgg cagggaaaat gttttttccg tacgctggcc actgctctgc
     9121 cggaccacca cagagaacaa ccgttcaccc gtcatgctga cgtcaatatc cgccaccccg
     9181 tcaaccaggc attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtccg
     9241 ccctggtgtg tggcatacgt gccccgggga aaggattttt gatcgtcaat ggcagggagt
     9301 atttccagtg ccgtggcatc acgcccgtcc tgcggagccg gcagggcact caccgcatcc
     9361 agaaccgcct tctgcagaac atcgggatca tagtcacgac catcacgcgg aacaggaata
     9421 tggcttaccg cctccttcac catctgttca agcatcggac gcacatcatc cggggtgata
     9481 cttttaccgt ccgccggctg cggaatattt gcgaccgcat cattcaccgc cttctgcaga
     9541 acatcgggat catagtcacg accatctcgc ggaacaggga tatggcttac cgcctccttc
     9601 accatctgtt caagcatcgg acgcacatca tccggggtga gacttttacc gtccgccggc
     9661 tgcggaatat ttgcgaccgc gtcattcacc gccttctgca gtacttccgg atcgtagtca
     9721 cgaccgtcac gcggaacagg gatatggctt accgcctcct tcaccatctt ctcaagcatc
     9781 ggacgcacat catcgcccgt cacgcacttc tgtaatacca ctgacaggga cgccagtttt
     9841 tcttcaaacg tttgtgcctg cgcggctatt ttcccctcaa atgtgcgctg taaatctgcc
     9901 agcactgcgg aaaattcttc acccagcgca cggataatgg acagttcacg ttcattcatt
     9961 ttttcagaat ccccctgaac atcgccttca ccgcatcatg ctctgtttca gtgattgcct
    10021 tattaccgtc agaggcagcg accgcctgag gcacggaagc tgttttaccg gacgacgcga
    10081 acggatcttc acgggcatca cggcgggaca acgcttccag actgtagttc tgctgctgaa
    10141 gatacagtgc atcaccgccg gccaggggcg gcaggttctc ccgtttacgg gcctcattgg
    10201 gcgtgagaag cgtatttttc accgcatccc ccagcgtttt catgcgccgc tcactgtcca
    10261 ttctcagcag cgtggtgaca tcaaattccg tactctcgtt ttcccccgtt tccagcgcct
    10321 catccagtaa cagctcaatg gactcaatca gtgtctgcag gcactgggaa taatactgct
    10381 gctccagcgc ctccacgttg tcactggaag gcggttgtcc cacgccaatc ttgtaggccg
    10441 ggacacggaa caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgtg
    10501 catccaccgg tgaaaacgtc gtggggttgt attttgcccc gttgctcaga atggccgttt
    10561 tccccgcatt ttcgcctgta tacccgctgt cccagttgct cttcagtttt ttcgcatttt
    10621 cttccgtaat actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa
    10681 aagacgtcga attttcctga atatgatgcc cctgcgtggc cgccagcccg gcggcataca
    10741 ccggcggcaa ccctataagc ggatgaaaaa aacagttaaa ccggtcgtgg atcacttccc
    10801 gggcaggcac cgtcaccgcc tcagggatcc cgcagttccg gtccggcgtg atgcggtaga
    10861 acacctcgcc gtcatccgcc accagaggtt caacccggtt ccagtccaga atacgcagtt
    10921 ctttgatctg cccccgggca ttacggattt tcagcaccac cgtattgccg tgacgcagtt
    10981 tggcgttcag ccacagttca aaaaactgga tgcggttctg ctgggcgttg ggacgacgac
    11041 agaggcgggc aatatccccc cggcgcgttt ccctgcgtat cccctgcgca tccgtctgca
    11101 tcaggcgcag ccgcattttg gcgatatcct gggatatcag cgaaatacat gcaaacaccg
    11161 catgaaagga gaggacggct tcaggatcgg ctttcacgcc ctgctgccag gcaccggaaa
    11221 agggctcagc caccgcctga aacaggctgg tccagcccgc ctctcttacg tcacgtcctg
    11281 atttctggtt ttttcgggtt cgccgtaaaa ggttccacat tcgccatgct ccgcatcacg
    11341 tttctttttc tgacctgccg gacgttgcac cgtgatgtac tccgcctttc ccaggcgaac
    11401 cagcacctcc gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt
    11461 gccctgcaga tatcggatct ttgccataac ctgttacggg aggcgcacgc ctcccgtcct
    11521 ccttatcaga ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc
    11581 gccgcggtac ggcgacgacg ccagttgatc cagcgctccg cacggatggc cacgctgcct
    11641 gtctggaaca tggaaaccag ctccaccggc gacggcgtgg tactgtcgcc ggtcggctca
    11701 gactgcattt ccagtgatgc ctcgcgggac atatccactg ccacgccgcc gtcatccgcc
    11761 agataaatat ccggggcatt caccagcacc agctggtcac ccacgtactg ggagacaatc
    11821 accggcagcc cctggaagga gccacccagc agggtcatgt ccgggtattc tttctgaccc
    11881 agcgcatttt tacgcatgga cagtgccagg gcattggtgc tggacatcag ccagaccgca
    11941 ccggtgggtt gcaggtttgc tgccacaaac tgtccaaacg cagcctctgc atccgcatcc
    12001 gggttaccgc ttgatgccgt gcccttcaca tcatgggtga tggacgccgg ggagacatct
    12061 gccaccgccg cttttttcgg gtccacaaag tctgtatcca gacgcgccac caccgcttcc
    12121 gccagcgcat tacggaccag tgcatcagca gccggactgg aaaaacggat caattcttcc
    12181 gtcagtaccg caatggccga caccttcgca tgactgaagg tgatggattc aaaatcaaac
    12241 ttcgtcaggg gttttgcctt accctcaccc acccagccgg cagcaccgcc ggacacctgg
    12301 gcgtgcacac ggatattgaa cggcacctga cgaagtgcag ggatcccgcc ctgaccaaat
    12361 cgcccgataa tggtctgagg acgcaggtaa tcaataaagt cctgtgcgta ttcctgatat
    12421 tcagacaggc tgcctgccca ctgcggatcc gtggtggtcc ccgcgcccac tgccgatttc
    12481 aggacatgat gcagacgact gtcatccgga tactgacgac gggccacttc cagggcttca
    12541 gagcggacac ctttagccgc agccagcgat ttggcaaaac gtgcgaaacc aatcccctta
    12601 tccagtttct gctccacacg gatcaccggc gcagaagcca ccgcggccac attcccgtta
    12661 ccggcctgtt tcaccggctg cgccgtggcg gccttaccgg cttccagttc acgcaggcgc
    12721 ttcaggtgcg catccacctg acggatttcc gctgcggtgt tgtcgtaatg ctcttcctcc
    12781 tccacatcca gcgtgcgccc ttcctctgcg gctttggtca tgacctcctc aagggaggct
    12841 gccagcgctg cacgcttgtt ttcaaaactt ttaatctgtt cgccaatatt cattatggtc
    12901 ttttccttat gaaaaacggt tgttgactgt gccgcagcgc cggcagaaga tgcgattttc
    12961 accaccggtt tccggttgcc ggacgcggca gaaaacgggc ggtcgtaaga tttaatggtc
    13021 cggatagtgc attccgcatt cgcgggcacg gtgacggcag acacctccat cagctcccag
    13081 cgcagaaaat gcactccgcc tccgtccaga aaggtgtatt catgaggacg gaagcccacg
    13141 gacagccccc tgaccagccc ggtcttaatg gccgcccaga cctcatccag ccgggcagcc
    13201 agttgcgacg gcatatccgg tacgggtttc accagtgttg ccgtgatttc cagcccttcg
    13261 ctgacccggc gcaccgtaca ctgccccacc gggcgggaat ggtcatgctg ccagagaaac
    13321 gggatcgcac tgccaaactc cgcgccctcc ggctccagga tgtcaccatc ccgatccgga
    13381 gaaggcgttg acgcaatccc ggtgatcacc cgctcatcct cactgaagga tttcaccgtc
    13441 agcagggagc aggcccgttt aagagtcaca tcagcctcct gaaaataaaa aaaccgccgc
    13501 agcggtccat gatggttaca gggtgaacag ggttatatga aaaaaaccgc atattctttc
    13561 tttttgggct ccggattcag ggacatcagg gataccgcat tgaacagcgc catcagcggg
    13621 tcaattttcc cccgtccgct ggcctgtttg gtaataagga tggcgttacc tttaggctcc
    13681 acccgggcat tgccaacgca ccaggccatc agtggctgac caccatgcac cagcactccc
    13741 tcagc
//
LOCUS       DAZGCQ010000126        13445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000126 DAZGCQ010000000
VERSION     DAZGCQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.126"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..983
                     /locus_tag="ACHK07_004226"
     CDS             <1..983
                     /locus_tag="ACHK07_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO8104486.1"
                     /translation="RSWLARVTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKAIDLTD
                     TRDYGSLVAASLGQEWSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVDQWNKA
                     FAGRYPFKATGSDASLPLLAQFLRSDSGRITTFLKTHLGGLVHQEGNHWVAEPSASQG
                     MVVSPDFLRAINQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQ
                     MESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRT
                     QLVWITADGNPLKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            986..1267
                     /locus_tag="ACHK07_004227"
     CDS             986..1267
                     /locus_tag="ACHK07_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104487.1"
                     /translation="MNRGIYPTPAFTEDLGADGHIFPLSENGSFTPVGVFAPMSEKTT
                     GKRPEGQPHSAGESMLLKWRPPQIVNSKDDASPEAVVRFMKETDKQEKE"
     gene            1264..2877
                     /gene="tssA"
                     /locus_tag="ACHK07_004228"
     CDS             1264..2877
                     /gene="tssA"
                     /locus_tag="ACHK07_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8104488.1"
                     /translation="MSSPESLLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLIRD
                     GEKGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAERPSFAGLCSALENRLARSGGMEALVPQNSSTRESGGE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLNGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDMMY
                     GDEAAIPAVVNDQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            2911..4680
                     /gene="tssF"
                     /locus_tag="ACHK07_004229"
     CDS             2911..4680
                     /gene="tssF"
                     /locus_tag="ACHK07_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8104489.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     SVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPVGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRF
                     ECPEKVDWSKAGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGW
                     CRPMGFDDNDCLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEI
                     DIVLSEAWSSGLPFETENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHT
                     EIYSVDNIHGSVKNGRHPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLV
                     LGGRSFELEQLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAP
                     TMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVA
                     VKHTLIRRFEKGFMLRGVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQL
                     TLVLQPTGKRLRWKENHSRYVPG"
     gene            4644..5732
                     /gene="tssG"
                     /locus_tag="ACHK07_004230"
     CDS             4644..5732
                     /gene="tssG"
                     /locus_tag="ACHK07_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8104490.1"
                     /translation="MERESQPVRTRLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDHPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            5766..6290
                     /gene="tssJ"
                     /locus_tag="ACHK07_004231"
     CDS             5766..6290
                     /gene="tssJ"
                     /locus_tag="ACHK07_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8104491.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINADGAQTPLATMVRVYQLRDRKAVDAADYQTLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            6295..8817
                     /locus_tag="ACHK07_004232"
     CDS             6295..8817
                     /locus_tag="ACHK07_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8104492.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIYWRTEMDDVRGLDTYIFADSQLHYQFDVQLPYSEPSGLFDGAAESVWDV
                     RTWHNVATGTVTTRDYNYRTATTPMNATVSVRNDAVTTGEHYRYAAPYREAGDDASPE
                     PETESGAFYARLHHERELNRSACIHLFSNAAHLSPGQVLEPQGDVITDLKEGIVLTLV
                     TYRGARDSRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQ
                     GRYRVKMDFDREGTEPGFGYLWLRMAKPCAGETLGWHMPLIDGTEVAIAYSNGDIDLP
                     YIAYALHDAEHPDLVNRDNHTRNILRTPANNKLRMEDRRNEEHIKLATEYGKTQLNSG
                     HLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLN
                     QQLQQLENAAERAQALKADIDSQIQMFEQRLKPLNEALVFSAPEGMALTSGEDMQMSA
                     ARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGPVEVQAQNGSMRLF
                     AEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKVEYGTTASYLRKVKRTMTAAAQ
                     SMSMAFPRMSTSHIYSAIYRLLDKHGNILVNIPYRLTTPSGQTVTGYSDNEGRSVPVY
                     TRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESLP"
     gene            8817..10106
                     /locus_tag="ACHK07_004233"
     CDS             8817..10106
                     /locus_tag="ACHK07_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO8104493.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            10119..11333
                     /locus_tag="ACHK07_004234"
     CDS             10119..11333
                     /locus_tag="ACHK07_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO8104494.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGGAVIGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMKWYRRFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKYGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            11359..11738
                     /locus_tag="ACHK07_004235"
                     /pseudo
     CDS             11359..11738
                     /locus_tag="ACHK07_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            11735..12238
                     /locus_tag="ACHK07_004236"
     CDS             11735..12238
                     /locus_tag="ACHK07_004236"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO8104495.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTELAAH
                     FRRNMNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWLTIVDGKAWSF
                     KQREFIC"
     gene            12232..12576
                     /locus_tag="ACHK07_004237"
     CDS             12232..12576
                     /locus_tag="ACHK07_004237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104496.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVCDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            12576..13364
                     /locus_tag="ACHK07_004238"
     CDS             12576..13364
                     /locus_tag="ACHK07_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO8104497.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQPRLNYLTRQ"
BASE COUNT         3129 a         3362 c         3995 g         2959 t
ORIGIN      
        1 tccggtcctg gctcgcccgt gtaacccaga tgcggctgaa acttcagcag gttaccagcg
       61 ccccggatcc gcaggcgatg tcgcagatgc tggcccgggc cgtgtttcag ggcaaagcca
      121 tcgatctgac ggacacccgc gattacggca gcctggttgc agcaagtctg gggcaggaat
      181 ggagtggatt tggtcagtcg ttgtttgtgc aaccgctgga tctggcctgg cggcaggtgc
      241 ttgtcccggc tgcgggcagc ctcaatgccc gctggcagac aaccatagtc gatcagtgga
      301 ataaggcttt tgcggggcgg tacccgttta aagccaccgg cagtgatgcc tccctgccgt
      361 tactggcaca gttcctgcgt agcgactcgg gtcggatcac gacgttcctg aaaactcatc
      421 ttggcggact cgtccaccag gaggggaatc actgggtcgc tgagccttcc gcaagccagg
      481 ggatggttgt cagtccggat ttcctccggg cgattaatca gctggcagag ctgtcagata
      541 tcgtgttctc tcagggcgat gcgggggttc acttcgaact gatggcgcgt ccttcacggg
      601 atgttgccag agtgcagctt acccttgatg aacagaatct ggattacttc aatcagatgg
      661 aaagctggca gagcttcacc tggccgggga atacttacta tccaggtgtg tcgctcagct
      721 ggcggagtgt aagcagtggt atgcaactct acgccagtca ccaggggaac tgggggttta
      781 ttcgcctgct ggacgaagcc ctgatcacgc cacttgatgc cagccgcaca caactggtgt
      841 ggatcactgc cgatggtaat cctctgaaat tcgtcatgcg cagcgagctg ggcgacggtc
      901 cgcttgcctt actgaagcta caggggttta cgctgcccac cgccattttt aacgttgccg
      961 ccggcacgga aggtgttgag taaaaatgaa caggggcatt tatccgacgc cggcctttac
     1021 tgaagatctg ggggctgatg ggcacatttt ccccctttca gagaacggtt ccttcacgcc
     1081 cgtcggcgtt ttcgccccca tgtcagaaaa aacaaccggt aagcgaccgg aagggcaacc
     1141 ccactcagca ggagaaagta tgttgctgaa atggaggcca ccacaaatcg tcaacagtaa
     1201 ggacgacgct tctccggaag cggtcgtgag gtttatgaaa gagactgaca aacaggaaaa
     1261 agaatgagtt cacctgaatc cctgttaacg gcctgcgccg ccagccagga agaaaggctg
     1321 agcctcattt cccgcgcaaa tgaggccctg tcgctctggg ataactggct gcatccgttc
     1381 acctccggat ctgaaaccgg ggacgatcct gcatacgatg ataattttca gctgatgcgt
     1441 gaagagatca ataagatttc aggtaccgat tcagcgctgt tgtgcgaact ggctcagaag
     1501 tgcctttgcg aatgcgccag agatattcga gtcgtgacct ggtacgtgct ggcccgtctt
     1561 atccgcgacg gtgaaaaagg gttgtccgaa ggcttattac ttcttgttgc gatgctgacc
     1621 cgttacggac aggcatgtca tccccggcgt cctgccgccc ggaaagctgc gctggagtgg
     1681 ctgaacagtg caaaagtgac tgatgcactg ctgttatggc ccggggtgga cagcaatgag
     1741 gccggtctga cggcaggtgc cataagcctg ctgggatccg ccgtggcaga ctggccggaa
     1801 gctgagaggc cgtcttttgc ggggctttgt tctgcgcttg aaaatcgtct ggcacgttca
     1861 ggaggcatgg aggctctggt cccccagaac agcagcaccc gggagtccgg gggggaatca
     1921 gctcccccgg actcaccaca actggcagca gtgacatcgg gccgcgactt actcgaccag
     1981 gccaggttac tttcccgctg gctgaacgga cagccgcagg gctggctggc ttctcaccgg
     2041 ctgatgaaga cggtgcggtg ggatacggtc gatcagctcc cttcgctgga cagtagtggg
     2101 cgtacccgcc tggtaccccc gaaaaccgga tatcgcgctc agcttaagcg actctggctt
     2161 cagaaaaact gggttgagct gattgaactg gccagtcaga tgtattgcga aggggtgaat
     2221 catttctggc ttgatcttca gtggtatctc tggcagggac tcagtcatgc tggtcagccc
     2281 tgggattcct ggtctgacgc tgtcctgtca gatctgcggc tgttgcttaa acgtctcccg
     2341 ggccttgaag ggctggcatg gaatgacggt actccctttg ctgacgaggt caccctcgcc
     2401 tggatcgctg aaaaagtgaa tgaagaagac atgatgtacg gcgatgaagc tgccattcct
     2461 gccgttgtta atgaccagac tgacgatgtg ctgatccttg agtctgaagc catggaaaag
     2521 ggagacacgg aagggcctga agcggcgctt gcctggctcc agtcccggcc ggggatggat
     2581 acaccacgtc atcgctggct gatacgtctg ctcatggccc gggtggcaga gcagtatggc
     2641 cggaacgata tggcgcttca tctgcttggt gaactcatta cccgtgcgcc acagctgacg
     2701 ctggaagact gggagcctgc tttgttgttt gaagtccggg cccggcgtct gaagctgcta
     2761 cggatgaaag ccggtcgcag cgaatctgat aaagcccgtc tgatgcctga gatggatacg
     2821 ttgctggcgg ggctgatcgc cattgacccg gcccgtgcca tggtgctctg tggctgatgt
     2881 tctctttaat cattttcggg aatgccgtgc atggatgatt taaccctgcg ctattacgaa
     2941 gcagagatgc gctatctgcg ggaagccggt aaagagtttg cccgtgccca tcctgatcgg
     3001 gcagcaatgc tgaatctgga taagcctggc gcctgcgatc ccagtgtgga acgtctgttt
     3061 gaaggctttg ccttcctgat gggacgtttg cgtgaaaagc tggatgatga cctgccggag
     3121 ctcaccgaag gtctggtgag tctgctgtgg ccgcactata tgcggactat cccgtcgctg
     3181 gccatcgttg agttctcgcc tgactggcgt agtctgcgtc aggccgaaat actggctgaa
     3241 ggcttctccg ttctgtcccg tcctgtaggg ccgcataaaa ccgcctgtca gtaccggacg
     3301 acccgcgatg tgttgctaca accgctgcac cttgcagatg cccgtctcca tactgaatcc
     3361 gatggccgct ctgccatccg cctgcgcttc gaatgccctg aaaaagtgga ctggagtaaa
     3421 gccgggatcg acaaagtcgc catttttctt aatgcagagg cgccggtaag cgctgctctg
     3481 catctggcaa tgacccggcg tgtacatgcc atgtatgcgc gtcatgccgg gacatatacc
     3541 ggacgtcatc agtttgacgg ctggtgcaga cccatgggct ttgacgacaa tgactgcctg
     3601 tggaagaaag ccgatacagc cttcagtggt taccagttgc tgctggagta tttcagcttc
     3661 cgtccgaagt ttatgtttgt tgaactgcgc ggcctcgaca ccatcgggct cactgcggcc
     3721 agtacctggt ttgaaatcga cattgtgctc agtgaagcct ggtcatccgg tttgcctttt
     3781 gagacagaaa acttccgcct gcactgtgcg ccggttatca atctctttac cctggaagcc
     3841 gacccgctga tccttaaccc gctggataac gagtacctgc tcaggccgct gcgtcttcag
     3901 gatggtcaca ccgaaattta cagcgtcgat aacattcacg gctcggtgaa gaatggcaga
     3961 cacccttatg tcccgttcac cagcttccgg caccggggcg gtatgatgcg ccacgatgct
     4021 cctgaacgtt attaccacac ccgcgtgaaa cggggaccct ccggcctgta tgacacatgg
     4081 ttagttctcg ggggacgttc gttcgaactg gagcagttgt ctgataaacc ggagtccctc
     4141 tcgatgcgaa tcaccgcgac caacgggcag cttccgcgca gggcgctgga aagtacgctc
     4201 ctggacaggg tggtcaaaac cggcaaagtc cccgtcagag tcctgaacct gaccgcaccg
     4261 acgatgccac tgtatccccc ggcaaatgac cggtttcact ggcgggtgat gagtcacctc
     4321 ggttcaaact ttctcagcat gatggacaat ccggaggtgc tgcgggggac gctggcgctc
     4381 tatgactgga ccgacgatga aatgaaccgc cgccggcttg aagccatcgt tgccgtgaag
     4441 cacaccctga tccgccgttt tgaaaagggc tttatgctcc gtggcgtgga cattgaggtc
     4501 acgctgaata tggataattt tgccggcgag ggcgatgtga atctcttcgg ggagatgctg
     4561 caccgtttct ttgcgctcta tgctgatgtt caccttttca accagctcac actggtactg
     4621 caacccacag ggaaacgact gagatggaaa gagaatcaca gccggtacgt acccggctga
     4681 cgcaggccct ggcccttaac aacgatatct ggcgggccag tttttatcgt ttctgccagc
     4741 tgctggagca ggaaaatccg gacgggccaa aactgggcac gaccagccat ccgggcgatg
     4801 atccggtgcg attcagaccc tggcccggga tgggattccc ggtcagcacc ctgaaagcgg
     4861 tggagatcga cgaagatcat ccgacattac ccccaacaat ccgcacgaca tttctcggaa
     4921 tgtatggggt ggactcacct ctgccgacgt cctggctgga tgatattacg cagcgccgtg
     4981 aaggacatga ggcactcacc tcatttctgg atattttcag ccatcgcatc accacgcagt
     5041 actaccggat ctggcgaaaa tatgcgtacc cggccacttt tgaggagggg gggcgcgatg
     5101 ccacatcgca gtgtctgctg gggctggtcg ggctcggtat acccggcacc gctgaacagg
     5161 tggcaacgcc ggtttcccgt ttcctggcct tactgggtgc catgcgatta cccacccgca
     5221 atgcggaagg catccgggct ctggtgagtc tgctggcacc ggatacccgc gcacttatca
     5281 ccgaacccga tccggttaag gtccatatcg gtaaccgcag tggactgggt gccggaaacc
     5341 gcatccgtct ttcgcagcgg gcgccgctgg gtaaaacgac aggggaagca tgcagccggc
     5401 tcctgatgac actggaaaca gcagatccga acgaagcgga aggctggttg cccggtggtg
     5461 tcctccatac cgatttgctg gtcctgctgc gtgtttatct cggctaccgc agtgacgtcc
     5521 ggctgcgcct tactgtcccc gtgcggttgt tgcctgagcc ccgcctgggc aaaggcaggc
     5581 ctgttcagct gggccgtacc ggtttgctgg ggctgaaagc gggcaaactc agcaatggcc
     5641 gggagagtct gacggtcagt ctgggctgct acgaagggct gaaatgtgct gcgctaccgc
     5701 ctgctgaaga cggacattat cgcttcgaat aatcacctgt tctctctcaa taaaggaacg
     5761 cctgaatggt aacagtaatc cgctggtggg cgccgatgtt tattgcctgt ggcctgctcg
     5821 caggctgcgg tctgacgcag accgtcacgg acggcacgat cagcatcacg aaatccatct
     5881 tctataaaaa aatcaaaacg ctgcatctgg attttacccc gcgtacggcc attaacgctg
     5941 atggtgcaca gacaccactg gcgacgatgg tgcgggttta ccagctcagg gatcgtaaag
     6001 cggttgatgc cgccgattac cagacactgc tgcgtaatgc tgatacggta cttaaggacg
     6061 acgtgctggc ctcaaaagaa ctgctggtga tgcccggcgg cagcgtgacg cttaatatgc
     6121 ccatggatga agatgcgcag tttgtggctg tggtgggact gtttaaccgg ccggatcaga
     6181 aggacaatcg ctggcgcctg gtactgaccc gtgatgacct caatccggat aagccccgca
     6241 ccattgaact gggtgatggc tggctcagcc ttgtgccggt aaaggagtga cgtgatggat
     6301 aaccggtcag cgttttttga cgggcagacc cgttattttc tggaaattaa tggaagtgca
     6361 gtaaagcctg atgttttacg ttttcgtggc agggaagcac tgagtgagcc gttccgatgg
     6421 gacatcgggt tcacctcccg gaatgcgaac attcctccgg aagaggtgct gatgaaatat
     6481 gcgtcgttgc ggatgcgtaa cggcaaaaat gtccatggca ttgtgacccg tctggaatgg
     6541 ctgtccacat cgaaagacca gtcccactac cggcttacac tcagttcccg tctggcactg
     6601 ctggagtaca cccggcagtg cgccgtattt cagaaccagt ccgtccctga ggtggtggag
     6661 caggtgctgc gcaaacacgg gctggagggc gctgattttg aattcagact ggaacgcgct
     6721 tacccgtcgc gtgaaattat cacccagtgg caggaaacgg atttgcagtt cattcagcgc
     6781 attctgtctg aggtggggat ttactggcgc acggagatgg atgatgtgcg cggactggat
     6841 acgtacattt ttgccgacag ccagcttcac tatcagtttg atgtgcagtt gccttacagc
     6901 gagccctcgg ggttgttcga cggcgcagca gaatcggtct gggatgtgcg gacctggcac
     6961 aacgtcgcca ccggcactgt caccacgcgt gattacaact accggacagc caccacgccg
     7021 atgaatgcga cggtcagcgt gcgtaatgat gcggtcacca ccggggagca ttaccgttac
     7081 gcagcacctt atcgtgaagc gggagacgat gccagcccgg agccggaaac cgaatctggt
     7141 gcgttttacg cccggcttca ccacgagcgg gaactcaaca ggtcggcctg cattcacctg
     7201 ttcagcaacg ccgcacacct ctctcccggg caggtgctgg agccgcaggg ggatgtcatt
     7261 acggacctga aggaagggat tgtcctcaca ctggtgactt accggggagc ccgggattcc
     7321 cggctgcatg tgtcagcatg gggaatgcct tacaccgaac gttactgttt ccgtccggca
     7381 gaaatcccgc gtccggtcat cccgggtacg cttccggcac ggattgagag tcgcgggaag
     7441 aacgatatct atgcccatct ggacgggcag gggcgttacc gggtgaagat ggactttgac
     7501 cgggagggga cggaacccgg attcggctac ctgtggctgc ggatggcaaa accctgtgcc
     7561 ggtgaaacgc tgggctggca tatgccgctc attgatggta ccgaagtggc gattgcatac
     7621 agcaatggcg atattgacct gccgtacatc gcgtatgcac tgcacgacgc tgagcatcct
     7681 gacctcgtga accgggacaa tcacacccgg aacatcctca gaacgcctgc gaacaacaag
     7741 ctgcggatgg aagacagacg caatgaggag catatcaaac tcgccacgga gtacggtaag
     7801 acacagctta acagcggaca tctggtggac agccaggggc aactgcgggg taaaggcaca
     7861 gagctgcgta ccgatgagtg gggaaccctg agagcgggga aggggctttt tgttgctgcg
     7921 gatgcgcagg cgaaagcaca gggcgaggca ctggacatgg atgccgcgct gaaggaaatc
     7981 gacaggctta atcagcaact gcagcaactg gaaaatgccg cagaacgggc acaggcgctg
     8041 aaggctgata ttgacagtca gatacagatg tttgagcagc ggctgaaacc gctcaacgaa
     8101 gcgctggtct tctccgctcc ggaaggaatg gcgctgacca gcggtgaaga tatgcagatg
     8161 agtgcagcac gaaatgtggc tgtcaacgcc ggaggtgata tcagtaccgg aacaatgggc
     8221 aacctgaccg cgctggccgg agagaagctg gggctgtttg cccggaccgg tcaacttagc
     8281 ctgaaatccg gggaagggcc ggtagaggta caggcgcaaa atggcagcat gcggttgttt
     8341 gctgagaaga agctgacact gagctcagcg agtgatatct cctttgccgg gaaaaaacgc
     8401 atcacgctga ttggcggggg aagttatctg cgacttgagg ccgggaaggt tgagtatggt
     8461 acgacggcgt cgtatctgcg caaagtgaag cggacgatga cggcggctgc gcagagtatg
     8521 tcgatggcat tcccccgtat gagtacttcg catatctact cggctatcta tcggttgctg
     8581 gacaaacacg gcaatattct ggtcaatatc ccctatcgcc ttaccactcc atctggtcag
     8641 acagtaacgg ggtatagcga taacgaaggc cgctccgtgc cggtttatac ccgccaacag
     8701 gaggatgtgg aactgcattt ttttgttaaa aaaccacaac ccgaagaaac gatgtggttt
     8761 gtgggggaaa ccgatacgca gcaactggaa accgaactca gggagagcct cccataatgg
     8821 aaatgcccga tttacccgag acaccgacat ctgatattcc caccggggcg aacaaccagc
     8881 cgtgggattg caccacgacg ggtgtcgaaa tggtcgaaaa gcagtcgcgt ctgccaacca
     8941 cggaaaaccg tcagtacctg catataaagg tggaatacgc catgcgtttt ccgcaactgt
     9001 cggcacaaat ccgccggaaa ggtaaaactt accagatgag ccttaagcag accgttatga
     9061 gtggtcttat tcgcgtggat gaaatcgccc gcaattactg ctggtattat aaggcggagg
     9121 tggcgtttga tatgcgcacc acgccaccac gcccttttct gagcagtacg ctgctggaaa
     9181 aagacggtgc tggccgtcgc cacacgacca atcctttccc ggtgcccggc aggggcattt
     9241 accgccgccc ggacgtgatt attgttaaaa ataaggagga ccgctggccc ggactggcgg
     9301 ggccggatac cgaaggcaat atgcacagcg ataacctggc ccgcctggtg gaggtgaagt
     9361 ttccgggaga taggctgtat gaagaccaat atgaagagta tatgcagatt gtcggtggaa
     9421 ttggtcatga ggatcgcatg accattctgg agatccacga ctgtcgcccg gaagagcacc
     9481 agtgggttga tcaggtgatc aacgtgacga tgaaagcctg gcagaccagc ggcgcgaaat
     9541 actggccgct ggctctctac ccgccggtct ttatgccccg tccgccatca acccccgttg
     9601 ctgccagaat agagccctgg acttacgccg gtaaagcagt atctgatctc agtgagggtg
     9661 tggtacgtgg tgtggctgaa ggctggaatg ccctgtcaaa agagacgcaa ctggtcctga
     9721 attcagcagc cagctggctt aacgacagcg gtgagtgggt ttaccggcag ggaagtgagg
     9781 tctgggtctg ggccagccaa accggtaagt cggtgaaatc ctggaccaac acccagatta
     9841 aggccgcatg gacagaaata cagcacgcca ccgatatgac gcttgatatg ctgaaacagg
     9901 tggactgggt acaggtttta accagtgtcg cgaaaacggt cggcgttatt ctggttgctg
     9961 tgggtgtcgg cggactggtc gtaacgctgg gtatcccgga agccattatt ggcgcgtttt
    10021 tactgattgt tcgtctggca ataagcgcct ggggaacact ggcgacgatt ctcggtacgg
    10081 gtacggcggt actggcagtt ggttaaacag gagcaggtat ggaattgcat gattttgagc
    10141 tgaactacat tgagaaatgg ctgccggaag cctccgtgaa atacaaagac ggcagtccgg
    10201 cagtcaatcc cggtctgatt atcaccgtct tttttaagga cggacatacg ccagaagtcc
    10261 gccgcaggat ggtggagtgc gtggatcgct tctatgggga gtttaaaccc tggctgaaaa
    10321 aaacgatcac caaaaactgg gttggcatca ctgataataa ctacgccaaa aagcggcagg
    10381 agatcctcga ttcaacgcct gaagagattt tttcctggta tctgagtagc gcagagaagg
    10441 attttttagc ccccgattac agtattttaa ttatggggaa aagaattttt cataatgaca
    10501 ataatcgttc agtcattaag ttaacactcc cgttgtcgct gctgaaagaa actgatggaa
    10561 agcagcgtta tgaagcctgg ctgatgtggc tgtgtgacac cttttcggtg gaaagtggtt
    10621 atgcagggtt gtcgtttgca cttccttatg cccgtgagcg aatgttcccc tgtgaattcg
    10681 ccctggcgca gcgtttttcc ggtgtgatgg tggattcact gggaacgctg gaaggcggtg
    10741 gggcagtcat tggtctgaaa ggtgcctgct ggtacaccat tctcggtacg ccgtggctgg
    10801 aaaaactggg gggggcagag agagtaaaac atcgtttagc taaaacgcct gaaatcagcc
    10861 tgctacccta taacaatggt gtcgtactta aagccggtga actccccccg ccactgggag
    10921 agatgaaaac agaaggttta tcgccgctgc tggtgaaggt gaaccaggtc attaagccag
    10981 tgcggtttaa tgaatcaaga tctcttcatt tctactctga atatgaagga cttcaattcg
    11041 acaaagaatc caccatgaag tggtacaggc gctttgacga agccagcgcc ctgctcgata
    11101 aagaagaaat ggggaaatct tgcgcccccg aacgcattaa atgctggacc gatgaaatag
    11161 cgccccatgc cggacaatgg gccgcgatgg ttaatggcac caccgagtac atccagaccc
    11221 gcgaagggca gaaaatgccc ccgtttgagg ataagtacgg taagcagcac agggcttgct
    11281 ggaaactact gaaacgagat gacaatggta gcgtttatac actacctgag taaggtcgtg
    11341 tgctgaatga ccgcgcaggc cggagagagg ctggggcttt ttgcccggac cggacaactg
    11401 agcgtcaagt ccggggaggg gccggtagag gttcaggcgc aaaacggcag catgcggttg
    11461 tttgctgaga agaagctgac actgagctca gcgtgtgata tctcttttgc cgggaaaaaa
    11521 cgtatcacgc tgattggcgg gggagttacc tgcgacttga agccgggaag gtcgagtatg
    11581 gtacgacggc aaagtatctg cgcaaagtga agcggacaat ggcggcgggg gctgcatcaa
    11641 taccagtcag gtccattgtg gggggaggta tttgcctgag ctgtctgatg aaggcggcaa
    11701 tgaatggcga cacctttgtt gtaaggggtg ggtcatgaac agcgtcagtt ttagccttgc
    11761 ggcacaacac ctgatttgtt caacaaaagt gacattattt gctctggtgg atggtctgca
    11821 atatgagcgg tatttcggtg agagtctgtc tgttcaacaa cctgccgccg tgcccctgtt
    11881 cgatacctgg cctgattcgc gtattgcatt tgcggggcca tgggttatgg agatgaacag
    11941 cataatggat ttcagagaaa ggctgtgtga gcttgaagct gcgcttccgt cagtttcctg
    12001 gatgatatct tcttcctcgc ttactgaact ggctgctcat ttcagaagga atatgaatac
    12061 agaactaccg gatggacgga ttgcgttgct gcgtttccat gatccgcgcg ttcagaagcg
    12121 cctgggagaa atgctgaacg accaacaaca tcgggagctg acggggttaa tgcaggagtg
    12181 gctgacaata gtggacggaa aagcctggtc atttaaacaa agggagttca tatgctgaag
    12241 ttgtctgaag agcagtgtgt tggtatggac aaaagggaag atgatgattt tgtttgcgac
    12301 accgcgctaa gactgaagaa gaaatttccg ggaattactg aatcagatga cacgttccat
    12361 gttcgcctga gaaaagcgct ggattatgcc aatacccttc cgcttaaaga gaaaaacgtg
    12421 aagcgggact tcttgtttct ggaggccttc tggcctggtt tttatctgaa acccgaagtg
    12481 gataaatggt tgagaacgcc gaatgcttat tcaactgacc agcgggttga agattataag
    12541 catgtgatga taaacagaga aaggagggag ctctgatggt tatggtcctg acaggggctg
    12601 gtatggggag cgcagctacc ggaggtgttg gtatggctgg tgcctcaacc ggaacagcag
    12661 tatttcccgg agcaggaagc cgcgatggag actgggaaga agataatata tcttatcctg
    12721 gctcaggcag tggttcgcgt cagggtaacg gagggtttta ttatcagggg aaacagtatg
    12781 atccaattct gaatgagccg atggttgtca gcgcagagac gcatggtgat ttctgggaag
    12841 atgatatgga tcattcagaa acagtaaact atgaaggtat tgatagcaaa gccgaagtac
    12901 aggcgaagac ggatgagaaa acgtgtgaga actgcccacc ggaagggaag gtaatgccga
    12961 tgataaggcg ttgctcgcgc tggagtatgg taacaatcag ctaccagaca agaatatgtg
    13021 gcacgtttta taatccggag acgcaacaga tacaggaatt taagtactgc ggcgtatcgt
    13081 ttgatggctg gaaagataag ctgtgtcagt tctgggaagc taaagcgcgg tatgaccaat
    13141 ttttcagggc tgatggttca aagaaaccct ggtggacagg taatctaagc gcaattaatc
    13201 aagcatcccg acatcaggcg gttgcaacag tcaatcagcc attgaaagtt gtctggatat
    13261 ttatgcaacc tgtaagttgt gagtatttca ggaaaatgtt taaagattac aaggatatta
    13321 ttacgaggtg gcaaccgaga ttgaattatt taacacgtca ataaatatac attctttgaa
    13381 agaaagagac ataacgatag acgattgttt gaatgaacta tacagaagta ccgtagttgt
    13441 taaaa
//
LOCUS       DAZGCQ010000127        13404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000127 DAZGCQ010000000
VERSION     DAZGCQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.127"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2209
                     /gene="crfC"
                     /locus_tag="ACHK07_004239"
     CDS             <1..2209
                     /gene="crfC"
                     /locus_tag="ACHK07_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO8104498.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEPLSE
                     IRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            2206..3084
                     /gene="rdcB"
                     /locus_tag="ACHK07_004240"
     CDS             2206..3084
                     /gene="rdcB"
                     /locus_tag="ACHK07_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO8104499.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            3348..4850
                     /gene="proP"
                     /locus_tag="ACHK07_004241"
     CDS             3348..4850
                     /gene="proP"
                     /locus_tag="ACHK07_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO8104500.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4962..5051
                     /gene="pmrR"
                     /locus_tag="ACHK07_004242"
     CDS             4962..5051
                     /gene="pmrR"
                     /locus_tag="ACHK07_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO8104501.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5027..6127)
                     /gene="pmrB"
                     /locus_tag="ACHK07_004243"
     CDS             complement(5027..6127)
                     /gene="pmrB"
                     /locus_tag="ACHK07_004243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO8104502.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6128..6796)
                     /gene="basR"
                     /locus_tag="ACHK07_004244"
     CDS             complement(6128..6796)
                     /gene="basR"
                     /locus_tag="ACHK07_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO8104503.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6793..8436)
                     /gene="eptA"
                     /locus_tag="ACHK07_004245"
     CDS             complement(6793..8436)
                     /gene="eptA"
                     /locus_tag="ACHK07_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO8104504.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8540..9877)
                     /gene="adiC"
                     /locus_tag="ACHK07_004246"
     CDS             complement(8540..9877)
                     /gene="adiC"
                     /locus_tag="ACHK07_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO8104505.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10014..10775)
                     /gene="adiY"
                     /locus_tag="ACHK07_004247"
     CDS             complement(10014..10775)
                     /gene="adiY"
                     /locus_tag="ACHK07_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO8104506.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11100..13367)
                     /gene="adiA"
                     /locus_tag="ACHK07_004248"
     CDS             complement(11100..13367)
                     /gene="adiA"
                     /locus_tag="ACHK07_004248"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO8104507.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3215 a         3291 c         3528 g         3370 t
ORIGIN      
        1 tgagttaagt caggaggctg aacgcctgtt acagctttct cgccaacagt tgcagttact
       61 ggaaaaaatg cctctctctg tacccggaga cgatgcgcca caacgggctt taccctggag
      121 tcagcctaat atcgccgagc gtcacgcgat gctgaataat gagttgcgta aaatttcccg
      181 actggaaatg gtgctggcga ttgtcggtac tatgaaagca gggaaatcaa ccaccattaa
      241 tgccattgtt ggtacggagg tactgcctaa tcgcaatcgc ccaatgactg cgttgccgac
      301 gcttattcgc catacgcccg ggcaaaagga accggtactg catttttcac atgtcgcgcc
      361 aatcgattgt ttaattcaac aattacaaca gcgcctgcgt gattgcgata ttaagcatct
      421 gaccgatgtg ctggaaatag ataaagatat gcgtgcgctt atgcagcgga tcgaaaatgg
      481 cgtcgctttc gaaaaatatt atctgggtgc ccagcctatt tttcattgtc tgaaaagttt
      541 gaatgattta gtgcgactgg cgaaggcgct ggacgtcgat tttccttttt ctgcttacgc
      601 cgccattgag catattcccg tgattgaagt ggagtttgtc catctggcgg ggctggagag
      661 ttatcccggt caattgacgt tactggatac ccccgggcca aatgaagccg ggcaaccgca
      721 tctgcaaaaa atgcttaacc agcagctggc acgcgcctcg gcggtactgg cggtgctgga
      781 ttatacgcaa ctgaaatcga tctccgatga agaggtccgt gaggcgattt tggcggtggg
      841 gcaatcggtg ccgctgtatg tgctggtcaa taagttcgat caacaggatc gtaacagtga
      901 cgacgccgac caggtgcggg cactgatttc cgggacgctg atgaaaggct gtattacgcc
      961 acagcagata tttccggtgt cgtcgatgtg gggctacctg gcgaatcggg cgcgccatga
     1021 gttagccaac aacggtaagt taccagcgcc agagcaacaa cgctgggtgg aagattttgc
     1081 ccatgccgcg ctcggcaggc gctggcgtca tgccgatctg gcggaccttg aacatattcg
     1141 tcatgctgcc gatcagttgt gggaagattc gctgttcgcc cagccaattc agatgttgct
     1201 tcatgccgct tacgctaacg cctcgttgta tgctctgcga tctgccgcgc ataaactgtt
     1261 gaattacgcg cagcaggcgc gggaatacct ggattttcgt gcgcacgggt taaacgtcgc
     1321 ttgtgaacaa ttgcggcaaa atatccatca ggtcgaagaa agtttgcagc tattgcaact
     1381 caatcaggcg caggtgagcg gcgagattaa acatgaaatc gagctggccc tgacctccgc
     1441 caaccacttt ctgcgtcaac agcaagatgc gctgaatgcg cagttagccg ccttgtttca
     1501 ggatgattcg gagccgttaa gcgagattcg tacctgctgt gagacactgt tacagacggc
     1561 gcagaacacc atcagtcgcg actttacgct gcgttttgcc gagcttgaat ccaccctttg
     1621 ccgggtgtta accgatgtta ttcggcccat tgagcaacaa gtcaaaatgg aattgagcga
     1681 gtcagggttt cgtcctgggt ttcattttcc tgtttttcac ggcgtagttc cccacttcaa
     1741 cactcgccag ctgttcagtg aagtcatttc gcgccaggat gcaacggacg agcagagcac
     1801 gcgtttaggc gttgtgcgtg agactttttc gcgctggttg aatcagcccg actggggacg
     1861 gggaaatgag aaatccccga cagaaacggt tgattacagt gtgttgcaac gagctttaag
     1921 cgcagaagtc gatctttatt gccaacaaat ggctaaagtt ctggcagagc aggtcgatga
     1981 atctgttacg gcaggcatga atactttttt cgctgagttc gcttcatgtt tgacggaatt
     2041 acagacgcgt ttacgcgaaa gtctggctct gcgtcaacaa aatgaatcgg tggtcaggct
     2101 gatgcagcag caattgcagc agactgtgat gactcacggc tggatttaca ccgacgccca
     2161 gctgttacgc gatgatattc aaacactttt cacggcagaa cgatattgac caagacgtta
     2221 cttgacggcc ccggtcgcgt gctggagtcg gtttatcccc gctttttagt ggatctggcg
     2281 cagggcgatg atgcccgcct tccacaagcc catcagcagc agtttcgtga acgactgatg
     2341 caggaacttc ttgcgcgcgt gcagcttcag acatggacga acggcggcat gttaaatgcg
     2401 ccgcttagcc tgcgactgac aatggtggaa aaactggcgt cgatgctgga tcccggtcat
     2461 ctggcgctga cgcagatcgc gcagcatctg gcgctgctgc aaaaaatgga tcaccgccag
     2521 cactctgctt tcccggagct cccccagcaa attgccgcct tgtatgagtg gttttcagcc
     2581 cgttgccgct ggaaggaaaa ggcgttaacg caacgaggcc tactggtgca ggcaggtgat
     2641 cagagcgagc aaatttttac ccgctggcgt gctggggcgt ataacgcctg gtcgttgcct
     2701 gggcgctgtt ttatcgttct cgaagagttg cgttgggggg catttggcga tgcctgtcgt
     2761 ctgggaagcc cgcaagcggt ggcgttgttg ctgggtgatt tgcgcgtgaa agcgacacaa
     2821 catctggcag agagtatcaa tgcggccccg accacgcgtc actattacca tcagtggttt
     2881 gcctcttcga ccgttccgac gggcggggat catgctgatt ttttaagttg gctgggaaag
     2941 tggaccacgg cagataaaca acccgtttgc tggtcagtga cccaacgctg gcaaactgtc
     3001 gcgctgggga tgccacgact ctgttcagcg cagcgtctgg cgggggcaat ggtcgaggaa
     3061 atcttctctg taaatttggc gtaaataatc agttacatca atgagtccta aacgaaatcc
     3121 atgtgtgaag ttgatcacaa atttaaacac tggtagggta aaaaggtcat taactgccca
     3181 attcaggcgt caactggttt gattgtacat tccttaaccg gagggtgtaa gcaaacccgc
     3241 tacgcttgtt acagagattg catcctgcaa ttcccgctcc ccttttgcgg ccgtcgcgct
     3301 gatttttctg gcgtttgcgg aaatgggcca actctgcgag gaaagctatg ctgaaaagga
     3361 aaaaagtaaa accgattacc cttcgtgatg tcaccattat tgatgacggt aaactgcgta
     3421 aagccattac cgcagcatca ctgggtaatg caatggaatg gtttgatttt ggtgtttatg
     3481 gttttgttgc ttacgcatta ggtaaagttt ttttcccggg ggctgacccc agcgtgcaga
     3541 tggttgctgc acttgccacg ttctccgttc cctttctgat tcgaccgctt ggcggactct
     3601 tctttggtat gttgggcgat aaatatggtc gccagaagat cctcgctatc actattgtga
     3661 ttatgtcgat cagtacgttc tgtattggct taataccgtc ctacgacacg attggtattt
     3721 gggcaccgat tctgctgttg atctgtaaga tggcacaagg tttctcggtc ggcggtgaat
     3781 ataccggggc gtcgatattt gttgcggaat actcccctga ccgtaaacgt ggctttatgg
     3841 gcagctggct ggactttggt tctattgccg ggtttgtgct gggtgctggc gtagtggtgt
     3901 taatttcgac cattgtcggc gaagcgaact tcctcgactg gggctggcgt attccgttct
     3961 ttattgctct gccgttaggg attatcgggc tttacctgcg ccatgcgctg gaagagaccc
     4021 cagcgttcca gcagcatgtc gataaactgg aacagggcga ccgcgaaggt ttgcaggatg
     4081 gcccgaaagt ctcgtttaaa gagattgcca ccaaacactg gcgcagcctg ttgacatgta
     4141 ttggtctggt aattgccacc aacgtgactt actacatgtt gctgacctat atgccgagtt
     4201 atttgtcgca taacctgcat tactccgaag accacggggt gctgattatt atcgccatta
     4261 tgatcggtat gctgtttgtc cagccggtga tgggcttgct gagtgaccgt tttggccgtc
     4321 gtccgtttgt gctacttggt agtgttgccc tgtttgtgtt ggcgatcccg gcgtttattc
     4381 tgattaacag taacgtcatc ggcctgattt ttgccgggtt actgatgctg gcggtgatcc
     4441 ttaactgctt tacgggcgtt atggcttcta ccttgccagc gatgttcccg acgcatatcc
     4501 gttacagcgc gctggcggcg gcatttaata tttcggtgct ggttgccggt ctgacgccaa
     4561 cactggcggc ctggctggtc gaaagctcgc agaatctgat gatgcctgcc tattacctga
     4621 tggtagtggc ggtgattggt ttaatcaccg gcgtaaccat gaaagagacg gcaaatcgtc
     4681 cgttgaaagg tgcgacaccg gcggcgtcag atatacagga agcgaaggaa attctcgtcg
     4741 agcattacga taatatcgag cagaaaatcg ataatattga ccacgagatt gccgatttgc
     4801 aggcgaaacg tacccgcctg gtgcagcaac atccgcgaat tgatgaataa gctgaaacgg
     4861 atggcctgat gtgacgctgt cttatcaggc caattgaact cttaaggttc acttaatctc
     4921 tgacgcgcat actctcctcc aggttaacgg aggagagtgc aatgaaaaac cgtgtttatg
     4981 aaagtttaac taccgtgttc agcgtgctgg tggtcagcag ctttctttat atctggtttg
     5041 ccacgttctg atctttcttc agccgtaccc aggcccgcgt gccggaagtc tcttgccggt
     5101 tttgcaggaa aaactgcccg tgatgcaact gtgtaatgcg gctgacaata cttaacccca
     5161 gaccaatccc gccataacgg ctgtccatac gtacaaacgc tttactcaac tccccgcatt
     5221 tactctcatc aatacctggt ccttcatctt caactgccat gaccgctccg ccatcttctt
     5281 gcagcttaat cataatgttg ctgccttgcg ggctgtaacg atgggcgttt tctaccaggt
     5341 ttcgcaataa catccgcagc agggttgcat caccctgaac ggtgatgtcg gcggcgctct
     5401 ctggcaatag cagggtttgc tgtcgctggt cgagcatggt actgagttcg tcatacgagg
     5461 ggagaatgac atcttccagc agttttacat gttgataatt accggaagaa aatgactgtc
     5521 cggcacgcgc cagttgcagc agctgggaga cgctctccat catctgatca agccgtgcca
     5581 ctaacggtgc tacatcaatg tgatgcgttt tcgccagcag ttccagatgc aaacgcaccc
     5641 cagccagtgg cgttcgcagt tcgtgcgcga cgtcagcggt aaacaacctt tcgttatcca
     5701 gcgtgctggt caggcgactg accagatcgt tcaacgccga aaccaccgct tcgatttcga
     5761 gggtggcgct gtgaatggca atgggcgtta agttgtcggc ggtgcgcgct tccagctctt
     5821 tttgcagctc cgccagcggg cgggtgatgc ggcgtaccgc ctgatagcag ataaatagcg
     5881 tcaggctgac cataaacacg ccggggacaa tcaggctggc gaccgcctcg cggatctcac
     5941 gcatgatgtg gcgatcgttg ttgcgattgt cgcgtagcgc ctgctcaaac agctgaatct
     6001 gctcggtact ttcatgccat agccagaaga cgctgatcag ctcaaacacc aacaaaatgg
     6061 ccccgatggt caatatcagc cgttggcgca gcgatattgg tcggcgcaga aaatgcatca
     6121 gattcaatta gttttcctca ttcgcgacca gcatatagcc aaagccgcgc acggtgcgga
     6181 tacgggcttt gcccactttg tcgcgcagat tgtggatatg cacttccagg gtgttggtcg
     6241 agggttcatt gtcccagtta tagatgtcgt tgtagagaat ttcccgatgc accggactgc
     6301 ctgctttgag cattaaccgt gacagcagag catattcttt gggcgtcaga atcaactctt
     6361 caccgcccat ccatacctga cggcgaccca tattcagcgt cagattgcca acaatcagct
     6421 cactttcgcc ctgattatta tggcgtcgta gcagggcgcg gatacgggcg tgtaactctt
     6481 ccagcgcaaa aggcttcacc agatagtcat cggcaccgac atccagcccg gcgattttgt
     6541 cggtcagcgt atcgcgagcg gtgaggatca gtactggcag ggtgtatttt ttctgccgga
     6601 tacgggcgag aaaatgcagt ccatcttcgt cgggtaaccc taaatccagt accaccaggc
     6661 tgtaatgacc ggcctcaagg ctttgttccg ccatccgcgc ggttgtcacg ccatcgcacg
     6721 cgtagccttc ggtttgcgcc gccagaatca atccctgcaa taacagcgta tcgtcttcaa
     6781 caatcagaat tttcattcac tcactctcct gcaagtttgc agaatatcat ccgcagcctg
     6841 gtaatacttc gtctcaacgc cagttaatcc caatagcgtg gagaataaat tgtcttgtga
     6901 atagtgttgc gtttgcgcct gtttttgcag gcagttctgg tcaacctgat accgtttttg
     6961 ataatcctcc gacagccaca gcagcattgg cacctgtttt tggctatccg gggcgatggc
     7021 ataaggcaga ccgtgcagat agatgccatt ttcacctaac gattcaccgt ggtcagaaag
     7081 ataaaccagg ctggtggtaa atttatcctg atgttctttc agcagattaa tcgctttatc
     7141 aacaatatag tcgacgtaaa ccagcgtgtt gtcgtaagtg ttcaccagtt gctctttgct
     7201 acaggtctgg atctcattgg tgtcgcaggt tggggtaaat ttcctgaact gcggcggata
     7261 gcggttgtaa taggtcggac cgtggctgcc gatggtgtgt aagacaatca cgccatcacc
     7321 ctgcaggtta ttgatgtact cttccagccc gtggaacagc acttcgtcat agcattcgcc
     7381 gttgatgcac tgaccaggca gattcagcgc ggtgacgttc tggtgaggca cgcggtcgca
     7441 ggcaccttta cagccgccat cgttgtcatt ccacagcacg ttgatgcccg ctcgctgaat
     7501 gatatccagc acgccttcct ggtgctgtgc cagctcttct ttgtaatgct cacgcggcat
     7561 atccgagaac atgcacggta ctgaaactgc cgttgccgtg ccgcaagatg cggtattagg
     7621 gaaatagacc acgttatctt tcgccagccg cgggttagtt tcacgcgggt agccgttgag
     7681 ggagaagttc tccgcccgcg aggtttcgcc gacaatcagg atggtcaaat ttttacgttt
     7741 ttcgttctgc attaacgggt tgcggtgggc gtcttcacca attcgcacca gcggtagatt
     7801 tgccagtcgc tgatgggagt accatgacca gctggcaaca atgctgttag aggggcttaa
     7861 ggatttcacc agctctttgt tattgcggaa caacgaggcg tagtctttat aaaacagtgc
     7921 ggcgaccagc aaaatcagta gtacagaaac cagaatattg gctccacgga aaagaacact
     7981 gcgcagacgc gaggtggcag gtttgatttt tatccagcag gcaatcagcg cagcaagcac
     8041 gccgctgaat cccagcgtta ataacatttg cggtgtcatc agcgcataac tttctgccgg
     8101 agtggtatca ataatattgg caatcatcga gcggtcgatg acgatgccgt aagtcattat
     8161 gaaatattgt gcagccgcgc caaccagaat aaacaggcag gccagtggtc gattaagcca
     8221 taagaaagag cttagtgtca ggacaatatt aatcacgctg aaagcgacga ccggcatcga
     8281 caagaaaacc agtacgttat gcagcgaatc cagcggcagc gcctgcaaca cctgtttaaa
     8341 aaaggcaata ttcaggcaga tagagatata aaaagcggcc aacaatagcc aggcgagtaa
     8401 attcaaagag ggtcttttta gtaggcgctt caacatcacg gtgtttccat cgaacaaagt
     8461 gcgcatatgc tcgcaaagca aaattaagcc aaccttaagt tcttaaggtt ggcttttatg
     8521 tttgctggat taaggggaat taatctttgc ttattggtgc atctaaggga tacgggtttt
     8581 tatgtagccg gttgtaattc agcgcataca tggcggtgat gaccatcagg gtgacaaatg
     8641 accacataac ctctttcgct ccggacccca ccacggccca gatgcagtag aggaaggcaa
     8701 tggtagtaac tgccagatat gccgggcgtg ctttaccaaa gtgaccgtgt ccgagcagca
     8761 gtaacgccgc acaggtgtaa agatatggca ccagtgtaaa gatgaccgag acggaagaaa
     8821 ccagaccgaa ctctttggtc gcgtttggtg aaatgctgct gagctggaag atggtcatca
     8881 aaataccgac gataatcagc cccgccactg gcgtacccgc tttatttaca cgggcaaaaa
     8941 ttggtgggaa cagtccgtca tcggcagcgg ctttcgccgt ttgacccgcc agcaacgtcc
     9001 agccgcccag tgaacctaag caacccgcag ctgcgcagaa ggaaacaatg gccccggcgg
     9061 tgtcacccaa cgccatccgt gcggcatcac cgaatggcga agcagaaacg cgcagtgcgg
     9121 cattagggat catccccata atcgcggtgg tagaaagtac atagcaaacg gcggcaatca
     9181 ataccccacc aatggtggcg atagggacat tgcgtttcgg gtttttcacc acacctgcgg
     9241 caacggaggc actttccaca ccgatgaacg accacagcgt aacgttaagg gtactttgaa
     9301 ttgcaccgaa ggtgcccagg ccgctgacgt tccatgccgc catataggtt tcaccacgga
     9361 accagaacca gccaaatacg gcaatcccga cgatggggat cagcgccagc acggtggcaa
     9421 ctgcctgcac acgggtgatc attttcggac cgacaatgtt cagcaggacg aagatccaca
     9481 gcaccacgac gcaggtgatg gttaatacca atggatcttt cagaatcggg aagaagtaac
     9541 ttaaatatcc tacgccaatg accaccatgg cgatattgcc gatccagcag gccagccagt
     9601 agaggacgtt ggtttgataa ccgagaaacg ggccaaagca gcggcgggcg taagcgtaag
     9661 aaccaccagg acttgggtcg aggaacgaca ttttggcgta taccatcgag agccccagtg
     9721 caccgataat cgtcaccaac catccataaa tggcaatccc gccagtagag gccaggtttg
     9781 caggtaacag aaaaacacct gaccccataa tattccccga caccatcagg gtgacgggga
     9841 ttaagcccac tttgtgagca tcagcatccg aagacataat taaactcctg cgaaggcgag
     9901 cttcgtgaca ataaattacg tcatatcata cgcctgcatt cgatagttga attattatcg
     9961 accgggttat cgctgatgcc gttattaata tagctcttcg ttaaggttaa aaatcaggcg
    10021 acagtgcgtt ctctgtgctg actaacataa tgcagcggcg tcataccgta gaagtcttta
    10081 aagacagaaa taaagtacga cgtactgttg tagccgcagg actgtgatac ctgagagata
    10141 tttttaccgt ccatcattaa ttcatttacg gcataacgca tgcggcaggt ggtgattatt
    10201 tggctataac tggtgttttc gcttttcaac tttttcttta acagacttgg gctaagacat
    10261 aaacaactgg ctaccatact aagattccag tctttgtgaa tatcgctttc aataatttga
    10321 taaacgctgt cacttacaca attacgtaac atatacatca ttaatgaaag gaatttttta
    10381 ctgtcgagaa aacgggatag caccgtgaat aataatgcgc gtgttcgttc cttttctgac
    10441 tcagtttctg cgggcatcat gctgtgttgc gcggcaacgc gaaagacgtc tggcgtcagg
    10501 catggcaggg tgagtatggg cgtagcgcta cgttgccata ctggtatttg cgagagatct
    10561 ttattcagaa accggaggta atctttgatg atgtcgtgac taatatgagc aaccaaagtg
    10621 ttgttaagag aggagacgtc gataatatta ttttcacaat ttaataacgc catatggtta
    10681 gctttaaggc taataggttc tttcccattc acccttatcc agacatcttt ttcagtcaat
    10741 aaaacaatac aaggttggtc gctgcaaatc ctcatacatg tactcctgag tgcaaataaa
    10801 agaccagttc tcgctaaagc aaagcgatac cgggaaatgg ttatataatt gtatgatgaa
    10861 tataaacatg cataaaaata acgagtttta ctaatattac aaatttttat agtaatttct
    10921 catgtacata tttttttaca ggataaataa caggcaaaaa aaagcgcggg gtggggccgc
    10981 gcaaaaagta tgacattttt gtttgcagca atttttatga aaacgttgcc atactcaaaa
    11041 ggcctaaggc caggcggcgt aatgttattt aaacaattac gccttcagcg gaatagtggt
    11101 tacgctttca cgcacataac gtggtaaata ccgtcaataa tttcagtccc ttcagtttcg
    11161 tgttcaaatc cagggaaatg gtggtcccag gattgcagcg agcgtaaata acttacttgc
    11221 ggactgtttt tatcgccgaa gttttcacca gacagcagca tcgggattcc tggcggatac
    11281 gggataactg agtttgccgc gatgcgtcct ggcagatttt caatggatac cagttcgaca
    11341 ttgttgtcga caatcgcgtt gtacgcttca cgcggggtga tttccgccat cggcaggccg
    11401 gaataggctt cgttcaaccg tgcgccaggg ttgttttctt tcagccaggc aaacatggtg
    11461 tcacccagat cgtgaatccc catgttcgcg taagtgtcag gatattgttc aacaagttcc
    11521 ggcatcacct gcgccagcgg tgtgttggcg tcatagtggc gtttgaagga gcaaagggtg
    11581 ttaaccagag tcccccattt cccacgggtt acgcccatag agaacaggaa cataatttgg
    11641 aagtcagtgg tgcgggtagg cacaatgccg tggcgaccaa gccaggcagt gaccagcgcc
    11701 gccggaacac cggtttcttc cagttcacca tcttcaccca ttcccggagc aaggatgctg
    11761 actttaattg ggtcgagcat actccagtta tccggaatat ctttgaagcc gtgccagctt
    11821 tcgcccggat gcattaccca gcagtcctga acggtggtca gcagtttggt tggtgcgtca
    11881 gcaaagtcat aggttttgcc ggtttgtggg tcggtgacga cttctttgtt ccacggtttg
    11941 aagaaccagc taccgtcagc ggtgaactct ttatatagcc gcgccatcgc ctgacggaaa
    12001 tcaaccgctt cgtcaatcac ttcctgtgtc agtgacaggc cgctgttgcc gtccatcatc
    12061 gacaccgcca cgtcgttgga tgcgcagatg gcatacagcg gggaggtggt ggcatgcatc
    12121 atgtaggcct ggttgaagcg ggagaagtta atcgccccac gaccttcacg tacatgaata
    12181 taagaagcct gtgacagcgc attcagcagt ttgtgggtgg agtgggtggc gaaaacggta
    12241 ggaccgttgt gatcgcctgg ttcgccgcgc atggcatagt gatcggcata gatcgggttg
    12301 aaacgtgcat agccgtacca ggcttcgtca aagtgcagac gatcggaggt tttttccagc
    12361 agatcctgcg cttctttagc gttataacac acgccgtcat aggtgcagtt ggtcaccacg
    12421 cagtaagacg gtttttgccc ggctttgtct ttggtcagcg ggctttcact gattttcttc
    12481 tgcaaggttt caggttgcat ttcctgcgga tagattggcc cgataatgcc gtagcggttg
    12541 cggcttggca ccatatagac cggtttcgcg cctgtcagca tcaaaccttg ttcgatggat
    12601 ttatggcagt tacggtcaac gaccacgaca tcgttatcgg tcatgcaagc ctgcatgatg
    12661 gtgcggttag agccggaagt accgacgact accgaccagg agcgatcggc accaaatacg
    12721 cgtgcggcat atttttcgct ttcgccaaat gcgccagtat ggtcaagcaa agaaccgagg
    12781 gaagttcgtt cgatgcccat gtcggtgcgg aacagatttt caccatagta gtcatggtag
    12841 aaacgtccgg cgggtgtttt ggtaaaacca acgccgccct ggtggcctgg cgctgcccag
    12901 gaatattcat ggatgtcact atatttcatc agcgcgctga acagtggcgg caacagctgc
    12961 tggcggtagc gggtcatcgc ggcaacggcg cgtccggcga taaagtcggc ggtatcttcc
    13021 agaatccagg cgaattcatc gacaagctcc agcagatccc ggtccattgc ggcgagggct
    13081 ttttcccgat cgcccaacag gaagaccggc acgttttgtt ggcgctcatg aagcttaccg
    13141 atcaattgtc tgacgttttg atgttcgtcc ggatgttcca tttgatagct gaacatcagg
    13201 cagtcaatgg cttcgtttga agagagaatg gcaaaaccat catcaaagga ggtggattta
    13261 atcacggtaa cattttgctg gcttaaagca tctgccagac gctcaacggc gttaccgacc
    13321 caggtgtctt gatggagaaa ctcgctttca acaattaata ctttcatcat tgcttacccg
    13381 gttatgaagg aatcttcgtt gcgg
//
LOCUS       DAZGCQ010000128        13395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000128 DAZGCQ010000000
VERSION     DAZGCQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.128"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            162..2540
                     /gene="ppsA"
                     /locus_tag="ACHK07_004249"
     CDS             162..2540
                     /gene="ppsA"
                     /locus_tag="ACHK07_004249"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO8104508.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVEQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2597..4243)
                     /gene="fadK"
                     /locus_tag="ACHK07_004250"
     CDS             complement(2597..4243)
                     /gene="fadK"
                     /locus_tag="ACHK07_004250"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO8104509.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWQEAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4300..4593)
                     /locus_tag="ACHK07_004251"
     CDS             complement(4300..4593)
                     /locus_tag="ACHK07_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8104510.1"
                     /translation="MSQNATVNVDVKLGVNKFHVDEGHPHIILAANPDIKEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4590..5879)
                     /locus_tag="ACHK07_004252"
     CDS             complement(4590..5879)
                     /locus_tag="ACHK07_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8104511.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIHPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMHHFRKIPALMENPRLFSQYPRMIADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5935..6873)
                     /locus_tag="ACHK07_004253"
     CDS             complement(5935..6873)
                     /locus_tag="ACHK07_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO8104512.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQVQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6893..7657)
                     /locus_tag="ACHK07_004254"
     CDS             complement(6893..7657)
                     /locus_tag="ACHK07_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO8104513.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DNVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7973..8884
                     /locus_tag="ACHK07_004255"
     CDS             7973..8884
                     /locus_tag="ACHK07_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8104514.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(8927..10078)
                     /locus_tag="ACHK07_004256"
     CDS             complement(8927..10078)
                     /locus_tag="ACHK07_004256"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO8104515.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10092..11687)
                     /locus_tag="ACHK07_004257"
     CDS             complement(10092..11687)
                     /locus_tag="ACHK07_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8104516.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(11832..12590)
                     /gene="aroD"
                     /locus_tag="ACHK07_004258"
     CDS             complement(11832..12590)
                     /gene="aroD"
                     /locus_tag="ACHK07_004258"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO8104517.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(12621..>13395)
                     /gene="ydiB"
                     /locus_tag="ACHK07_004259"
     CDS             complement(12621..>13395)
                     /gene="ydiB"
                     /locus_tag="ACHK07_004259"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO8104518.1"
                     /translation="KAGLPFTYMAFEVDNDSFPGAIEGLKALKMRGTGVSMPNKQLAC
                     EYVDELTPAAKLVGAINTIVNDDGYLRGYNTDGTGHIRAIKESGFDIKGKTMVLLGAG
                     GASTAIGAQGAIEGLKEIKLFNRRDEFFDKALAFAQRVNENTDCVVTVTDLADQQAFA
                     EALASADILTNGTKVGMKPLENESLVNDISLLHPGLLVTECVYNPHMTKLLQQAQQAG
                     CKTIDGYGMLLWQGAEQFTLWTGKDFPLEYVKQVMGFGA"
BASE COUNT         3244 a         3412 c         3330 g         3409 t
ORIGIN      
        1 cgaagagagc agatttgcgc aacgctggga tcagtcttaa aaagtaaaaa aatatatttg
       61 cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat gcgcagaaat
      121 gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac aatggctcgt
      181 caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg gttgggggca
      241 aaaatgcctc cctgggtgaa atgattacta acctttccgg aatgggtgtt tccgttccga
      301 atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc ggcgtaaacc
      361 agcgcattta tgaactgctg gataaaacgg atattgacga tgttactcag cttgcgaaag
      421 cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag ctggaaaacg
      481 ccatccgcga agcctatgca cagctttccg ccgatgacga aaacgcctct tttgcggtgc
      541 gctcctccgc caccgcagaa gatatgccgg acgcttcttt tgccggtcag caggaaacct
      601 tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt gaaacatgta tttgcttctc
      661 tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac cgtggtgtgg
      721 cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcatct ggcgtgatgt
      781 tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc gcatggggcc
      841 ttggtgagat ggtcgtgcag ggtgcggtta acccggatga gttttacgtg cataaaccga
      901 cactggcggc gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa aaaatccgca
      961 tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac gtaccgcagg
     1021 aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca aaacaggccg
     1081 tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa gatggccaca
     1141 ccggtaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc ggtcaggtca
     1201 tggagcgtta tacgctgcat tcacagggta agattattgc cgaaggccgt gctatcggtc
     1261 atcgcatcgg tgcgggtccg gtgaaagtca tccatgatat cagcgaaatg aaccgcatcg
     1321 aacctggtga cgtgctggtc actgacatga ccgacccgga ctgggaaccg atcatgaaga
     1381 aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg atcatcgctc
     1441 gtgaactggg cattccggcg gtagtgggct gtggtgatgc aacagaacgg atgaaagacg
     1501 gtgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat gcggagttgc
     1561 tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg ttgaaagtga
     1621 tgatgaacgt cggtaacccg gaccgagctt tcgacttcgc ctgtctgccg aacgaaggcg
     1681 tgggacttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac ccacgcgcac
     1741 tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag atgatgaaag
     1801 gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc gcgacgctgg
     1861 gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa tcgaacgaat
     1921 atgccaacct ggtcggtggt gagcgttatg agccagatga agagaacccg atgctcggct
     1981 tccgtggcgc gggacgctat gtttccgaca gcttccgcga ctgtttcgcg ctggagtgtg
     2041 aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc atgatcccgt
     2101 tcgtgcgtac cgtagagcag gcgaaagcgg tggttgagga actggcgcgt caggggctga
     2161 aacgtggtga gaacgggctg aaaatcatca tgatgtgtga aatcccgtcc aacgccttgc
     2221 tggcagagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac gacatgacgc
     2281 agctggcgct cggtctggac cgtgactccg gcgtggtgtc tgaactgttc gatgagcgca
     2341 acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc cgcgaagaaa cagggcaaat
     2401 atgtcgggat ttgcggtcag ggtccgtccg accacgaaga ttttgccgca tggttgatgg
     2461 aagaggggat cgatagcctg tctctgaatc cggacaccgt ggtgcaaacc tggttaagcc
     2521 tggctgaact gaagaaataa aataaatacc cggcggcgtt ttgtcgccgg gtttatgtga
     2581 tccccgaaga tgaaacttat tcaatctctt cacagacatc ctgagttaat cgccgcataa
     2641 tatctttcct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt agtttttcga
     2701 ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta aaaaaagcca
     2761 ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa gcgcatgaac
     2821 gttcacctaa gcgttcatcg ggcattgcaa ccacacaagc atcgtgaatt ttaggatgct
     2881 gtaataaaat atcttccact tcacggctgc taatattttc gccgccgcgg acaataatat
     2941 ctttcttgcg cccggttatt tttatatagc cagcctcatc catgcggcag agatcgccgc
     3001 tgtaatacca gccttcttca tccagggcac gggcggttaa ttcaggttca tcaaaatacc
     3061 ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc ggtaaggtct
     3121 tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca tcggtgtgca
     3181 taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt tctgtggaac
     3241 cataaacact taataattta atgccgcgct gctggcattc acgcgccact tttttgggga
     3301 ttgtggtacc gccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt tgtttctcta
     3361 gtaaattcaa aagatcatag acaaacggcg ttgcgccgag catacaggtg caacgctgct
     3421 gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg ctgcgagcgc
     3481 caattaagaa tggtgccgtt acgccatgca gaaagcccgt tgcgtggccg agtggcgcag
     3541 gcatcataaa gacatcctgc caggtcagat tcagccgcgc gcaataagcc cgctcactgg
     3601 cgagaatatt gttatgcgtt agcatcacgc cctttggcag accctcggtt ccggaggtaa
     3661 acagcaccgc ggctaattca tcgccgtgga ccgttatcgc cgtggtcagt ggggtattgt
     3721 cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg tccacgccga
     3781 caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct accggacgcg
     3841 tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttattg agcacccaca
     3901 ccagttctgc ttcctgccag gaaggcaaca gcggcacgga aaccgcaccg attttcaggc
     3961 aggcaagata gataacggta aattcacacc agcctggcag ttgaaatgcg atgcgatcgc
     4021 ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg tgatcgagcg
     4081 cgctataggt gtacgatgct ccatgattat cgaccacggc aattttgtct ggcatcgcac
     4141 gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct tgctgacgat
     4201 acgccgcacg acgttgttcg ttaaacgtta atgtcacttt cattttggac tctcgaaaca
     4261 gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc gaaaatcaat
     4321 accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttaccgc acaacacccg
     4381 acaggtgccg cactccagac aaccggcaga atcaaaatga atgttgccgg cgtcatcctg
     4441 cttataaagt ccggcagggc aggctttcat taatttttga aattctttga tgtcgggatt
     4501 tgccgccaga ataatatgtg gatggccctc atcaacatgg aatttattga cgcctaattt
     4561 gacatcaacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt aatgccatct
     4621 ttcagcagat tgatcagccc gattttcttc gcgtgtccca tgatcatttt gcgtaccggc
     4681 tggtttggtt tgccgtcaat ggtgaacata tcgttcatga tgtcggcgat catgcgcgga
     4741 tactgactaa acaggcgcgg attttccatc agcgccggga tcttgcgaaa atggtgcata
     4801 tcgcgcataa cacaactttg ttccagctca cgtttgtatt gcgcaagact gctggcggag
     4861 aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc cgatgcaatg
     4921 gctaaatcca tgccgcggac cgtaaaaccc aaattcaggc agaagcctgc ggcgtcacca
     4981 acgatcatca cgccctcgtt aaccagttgc ggcaccattg ccagaccgcc ttccggcacc
     5041 atatgcgcgg aatattcaag cagtttgccg ccgctaatca gcgggtgaat ggcggggtgt
     5101 tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat atcacccaat
     5161 ccacaaacca gccccaacga tatggaatcc ttattggtat agaggaatcc gccacccatc
     5221 aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc cgtaatatta
     5281 aagcgatcgt tgatctgttc tggtgtgagg ccaataacct ctttaacacc aacagcgtaa
     5341 tgatgcggat cggaagcggg aaccattccc agcgaacggc caagcatcga gttaacgcca
     5401 tcagccagaa tcaccacatt cgcttcgaga atatcatccc cagcctggac gccagtgacc
     5461 ttgtttcctt cacgaaccag cgcatcgacg cgaactcccg ggataaactg tgcgcccgcc
     5521 tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt ataagatgcg
     5581 tgttgcggaa catctggttg ctcgcggtga aaatcgaggg taacggcgct ctcttcggtt
     5641 aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgaggc tgcaaagcct
     5701 ggaatgattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt tttacatccg
     5761 gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgtgccat gaccagtgcg
     5821 gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc atccgacatg
     5881 cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg ggaattagcg
     5941 gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca gaccgtagtc
     6001 ggcatagttg aagattggcg catttttatc tttattgatg gcgacaatca cttttgcgcc
     6061 gttgccgcca accatatgtt ggatctgccc ggagatcccc agcgtcaggt agagatcgga
     6121 tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt tctcgccttc
     6181 ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt cgtggaccat
     6241 tttcaggtca tcctgcgctg ccagaccacg accgacgcca accacacgtt ttgctttgct
     6301 caggtccacg ctgcttgccg ctttcgcacg gcgctcgcga cagagaattt catgacgcgg
     6361 agcaacataa ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg tgcacggttc
     6421 ctgaacaccg ggtgcaaggg taatgatcgc tagcgagctg ttgatctttt cctgagcgaa
     6481 cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa tatccaccgc
     6541 cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg ccagagcttt
     6601 accacgtttc gtcgcggcca gtagcaacat agcggggtgt ttatctttca gcagagcagc
     6661 aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa gaacataaat
     6721 acattttgga ccataaggca taactgcctg cacctggtcg gtattttgta caatggcata
     6781 cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt caggattatc
     6841 gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg cttcagttca
     6901 gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg ttatcgagaa
     6961 tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc ggtcagttca gcaagtggtg
     7021 cgctctggct ccagccaata tcacttgcct gccactgatt taccggtttt ttacccgcgc
     7081 cgagaatggc tttcatcgaa ggaatgcgtg gcacgttgat atcggaggtg acgcagagca
     7141 cggctggaac agagagttca ataacttcaa cgttatcttc aagcgtgcgt tcaatcacca
     7201 gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt tgcagaattt
     7261 ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca ccaaagatca
     7321 gtaaatcgaa gccgatcttt tcaactgctg ccgccagcgc ttttgcggta tcgagaggca
     7381 gtgcatgttc aagttgcgca tcctgcacca aatacaggct gtgcggcccg cgggataaca
     7441 cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc gcggctatct
     7501 catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg aactggctga
     7561 ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca atgtcctgtt
     7621 cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg agtcgcatac
     7681 ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg
     7741 gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa tgcgatcgct
     7801 aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat cttgcattca
     7861 gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt
     7921 attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg tcatgtatca
     7981 acgctgtttt gataatgcca gtgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc
     8041 acgttttgca tttagcgatg atccaaaatg ggagtctgga catcacgttc atgacaatga
     8101 aaccgagctg atttacgtca agaaaggggt ggcaaggttt accatcgatt cttcgttata
     8161 tgtcgcgcat gcagatgata tagtggtgat agaacgcggc aggctgcatg cggtggcctc
     8221 tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg
     8281 ggctgaggaa aatcatctac tgcaaccgca ttcttgtccg gtaattgccg cggggcaggg
     8341 aaaagaagtc attaaaacct tatttaatga gctaagtgtg attttgccgc aaagtaaaaa
     8401 tagccaaaca tcttcgttat gggatgcatt tgcctataca ttagcaattc tttactacga
     8461 aaactttaaa aatgcttatc gttcggagca ggggtatatt aaaaaagatg ttctgataaa
     8521 agatattctt ttctatctga ataataatta tcgcgaaaaa atcactttag aacagttatc
     8581 gaaaaaattt cgtgccagcg tcagttatat ttgccatgag tttaccaaag agtatcgtat
     8641 ttcccctatt aactatgtta ttcaacggcg tatgacggaa gcgaaatggt tactcactaa
     8701 tactgaatta tcacaggcag agatctcctg gcgtgtgggt tatgaaaatg tcgatcactt
     8761 tggcaaactg tttttgcgcc atgtcggctg ttcgcccagc gattaccgca ggcaatttaa
     8821 aaactgtttt gcggaacaag aaatcctatc tgaatttcct caaccggtaa gtcttgccgg
     8881 ataaatctgg cggcgcagtt tgttaagaag ctgcgccgcc tgcagattat ttgttctgat
     8941 agtctttcag gatctgccga cctgctacgt aaatcataat ttcgtctgta ccgccgccga
     9001 tacgttcaca acggacatca cgccagaagc gggagacgcg cgcttcatcg gtatagccca
     9061 gaccgcccat gatttgaatc gcatcatcaa tgacttccat tgcggtacgt gcgcaataca
     9121 gttttgcaag cgccgcgctg gtgcgcagtg actgatgctg atcggcttgc catgccactt
     9181 tcagcaccat gttgcgcatg ttgtcaatct taatcgccat cagcgccagt ttttcctgga
     9241 tcatctggtt atgaccaatg ggcttaccaa aagcgatacg ttggttggca tagcgggcgg
     9301 catcttcaaa ggcgcattcg gcaaagccgg tgctgcgcgc ggcgttgatc aggcgctcca
     9361 tctcaaagtt gtacatcaca ttgaggaaac ccattccttc ttcgcccacc atgtcgctct
     9421 cttcaacttc cacgttgtcg agatagactt cgcaggtgct gagcatatgc cagccgattt
     9481 tatgcagcgg gttaatctta atgccgggct tactggagtc gacccaccac agggtgaagg
     9541 cttttttggg atctttcggt tcaggatcgc gcgccagcac cagcatatac gggtactctt
     9601 tcgcaccggt aataaaggtt ttctgtccgt tgatgtaaac cttgccgttt ttacgcgtgt
     9661 aagtggtagt ggcactgttg ttgtctgagc ctgcgcctgg ctccgtcaac gccagggcat
     9721 aggcgggatc acccgtttcc agtgtgcttt ctgccgtttt acgtagctgc tctgcagaac
     9781 cgaaacggcg catactgtga atacattgac cgttggtaat caaaaatgcc ggagcaccgc
     9841 attttgacac ttccatcagt gccagcattt gggtgacgta atccgcaggt ataccaccaa
     9901 attcttccgg cacgccaagc atggaaatac cgttatccgc cagtgcccgc ataaattcac
     9961 gcgggtatgt cccgttttga tcgcaggtgc ggaaatactc ttccggaaag ttagtcgtaa
    10021 tcagttcgcg aatactggcc agcagcagtt cttgttcttc agttaaagaa aaatccatcg
    10081 tgatactcct gttaatgagc cgcttcaggc aggacaaaac ccatcgccgc atcgataaat
    10141 aatctttcgt ccatcagttt gagttctggc gaaatcactg gggtgaaatc cattttgtcg
    10201 agaatatctt tttgtaaatc gacgccaggg gcgatttcga ttaaatgcag gccgtcttct
    10261 ttcagcgtga atactgcgcg ctcagtgata taacgaacat ccagccctcg ctcgagagcg
    10321 atttttccgc tgaaagtaat ttccggtagt tcccgaataa atttcttcac ccgtccttcc
    10381 tggacgatat ttaatttgcc gtcggtaatt tctgttttta aactgcccgc agttaatgtg
    10441 ccgcagaaaa tgattttctt cgaagtggca ctgatatcaa taaatccacc ggtacccatg
    10501 attttgccat tgaatttatg cacgccgacg ttaccgtgct ggtcgacttc agcaaaactc
    10561 aaataacaaa catccagacc accaccgtga taaaaatcaa actgggacgt catatccaga
    10621 atggcgcggg tattcacgtt cgcgccaaag gcgataccct gtgaagtaat tccgccaatc
    10681 ggacctgttt ctaccgtcag aataaagtca tcagcacaac cttcttcccg ggcgaccagg
    10741 ccaatgccgt cagcaatacc gacgccgaca ttccccaccg cgcctttacg catttcgaat
    10801 aatgcgcgcc gcgcaactaa tttacgttgg tttaggggca ggctaagttt ggtactgtca
    10861 tcaagtgtga agtcaccaga aataaagcgg ttaaccggtg caccgccata cagttgggtt
    10921 tgatccggat cgaccaccac aatatccacc agataacccg gaatacggac agatttagga
    10981 tgcagcgtgg ctttcttaac cattttctgc acctgcatca tcacaatacc gccgttattg
    11041 tgcaccgctt gggcaataac caatgcgtcg agatacatca cctcatcttc aaaagtggcg
    11101 tagccttcac tgtcgcaggt ggtagcgcga atgaaagcaa tatctggcgc aatcgctttg
    11161 taatagagat attctttgtt atcaaactcg accagtttaa tcaggtcttc tttagtgact
    11221 tcattcagtt tgccgccttg ctggcgtgga tcgacaaatg tcccgatgcc aatatcacta
    11281 ataataccag gctggtgggc tgcggcggcg cgtaaggttt gtgtaagtac accttgtggg
    11341 tagttataag caataatttt attttgttct gcgagttcag aaatacgcgg cgattgtccc
    11401 cagtgaccac ataatgccca tttcaccaga ccttcttgcg ccagaggact aataccacgg
    11461 tcggcgcgat cgccaagccc tgttggacta ataatcgata aattacgtgg cgtttgagtc
    11521 tgtttatatt tatcagcaag agcagtaatt aacgtggtgg cttccagaat accgccgcca
    11581 gcgcctaaca cacaaagtgt tgcttcgtcg ggaatataat tcaccgcttc ctgtgccgac
    11641 aggaccggca ctcgtccatt aatacgaggt ggttttacag gtttcattgt taattcctga
    11701 ttcaggtgtg attttcccta cgccagatgg ataattagcg cacagagact cacgaaatat
    11761 ttttattaaa tacggttgaa tatatttttt tagttcggcg ggggaggata ttcccgccga
    11821 aatattattg cttatgcctg atgtaaaata gttaatactg tgcgcaaatc atttaccgag
    11881 atttgccctg gcgcagaggc tttttttacc gcaccaaaag ttgccgccga cccaaatact
    11941 tcaccagcca gacgagaaat tacgccagtt tttgccattg acatcgtgat gattggacga
    12001 tcggcatact gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta
    12061 ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga
    12121 cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact
    12181 ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa cctgatcatc gccggtaaat
    12241 aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata
    12301 taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc
    12361 agcggttttt ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca
    12421 ttggagaggt cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga
    12481 taggcgagag cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt
    12541 ttaggtgcgc ccgtaccaat gacgagatct tttacagtta cggttttcat tttttaccct
    12601 ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga
    12661 gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg
    12721 tatccatcaa tcgttttgca accagcttgt tgcgcctgct gcaataactt cgtcatatgc
    12781 gggttataca cgcattcagt gaccagaagt cccggatgta acagactgat atcattaacc
    12841 aatgattcat tctcaagggg tttcataccc acttttgtgc cattggttaa aatgtcggcg
    12901 gaagccaggg cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa
    12961 tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga
    13021 cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt
    13081 gaggcacctc cggcccctaa cagcaccatt gttttgcctt tgatatcaaa accgctctct
    13141 ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc cgcgcaggta gccatcatca
    13201 ttaacgatgg tgttgatggc acccaccagt ttggctgccg gcgttaattc atcaacatat
    13261 tcacacgcca gttgtttgtt cggcattgat acaccagttc cgcgcatttt tagagctttt
    13321 aatccttcaa ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat
    13381 ggcaatcccg ctttt
//
LOCUS       DAZGCQ010000129        13385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000129 DAZGCQ010000000
VERSION     DAZGCQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.129"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            294..1238
                     /locus_tag="ACHK07_004260"
     CDS             294..1238
                     /locus_tag="ACHK07_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104519.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDILLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1319..1741
                     /locus_tag="ACHK07_004261"
     CDS             1319..1741
                     /locus_tag="ACHK07_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104520.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            2289..2738
                     /gene="glmS"
                     /locus_tag="ACHK07_004262"
     CDS             2289..2738
                     /gene="glmS"
                     /locus_tag="ACHK07_004262"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO8104521.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVENKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
     gene            2738..4126
                     /gene="glmL"
                     /locus_tag="ACHK07_004263"
     CDS             2738..4126
                     /gene="glmL"
                     /locus_tag="ACHK07_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO8104522.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4145..5590
                     /locus_tag="ACHK07_004264"
     CDS             4145..5590
                     /locus_tag="ACHK07_004264"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO8104523.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNAGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5590..6831
                     /locus_tag="ACHK07_004265"
     CDS             5590..6831
                     /locus_tag="ACHK07_004265"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO8104524.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     TVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6835..8205
                     /locus_tag="ACHK07_004266"
     CDS             6835..8205
                     /locus_tag="ACHK07_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO8104525.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAEIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8202..8519
                     /locus_tag="ACHK07_004267"
     CDS             8202..8519
                     /locus_tag="ACHK07_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO8104526.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8680..9960
                     /locus_tag="ACHK07_004268"
     CDS             8680..9960
                     /locus_tag="ACHK07_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO8104527.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            10068..11720
                     /locus_tag="ACHK07_004269"
     CDS             10068..11720
                     /locus_tag="ACHK07_004269"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8104528.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIIASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDICAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11730..12332
                     /gene="cobO"
                     /locus_tag="ACHK07_004270"
     CDS             11730..12332
                     /gene="cobO"
                     /locus_tag="ACHK07_004270"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO8104529.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12366..13271)
                     /locus_tag="ACHK07_004271"
     CDS             complement(12366..13271)
                     /locus_tag="ACHK07_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8104530.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
BASE COUNT         3633 a         3249 c         3171 g         3332 t
ORIGIN      
        1 accaattgat tagagtagcc aatttgttgc tattttacat aacatatttt tatcaaagtt
       61 aattgattgt tgcctttatg agcaccgacc gtaagcccgt tatgttattg tttcattagt
      121 aataatcact taaatgtatc gatgaaacag gtatgactta ccgggagtga ttgtttctga
      181 gtgaacagag gccactttcg cctgataaat gaaaaatgag cgatttttgc aagttagttt
      241 ttctatagcc gagtacgctc agcgcctcgc ttgattacaa tgaggttgct gctatgagtc
      301 ctgataatat cggttcggct gtcaatcgct tctcaggttg gccatctaat gatcctctaa
      361 aaacagatga agaatcaatg acatcacccg tgactgtgca agtaataaag atgattgccg
      421 ccatccttga tccacatcgg atagtcgttg gaattgatac actttataca acgcagcgcg
      481 aaacatttac ctcagagagt caacagctca ttgaatcagc attgcaaaca cttcatgtga
      541 tgaatttaaa taacctggaa acaaatcgcg atgaagaagg tcactatctg gcttttgaat
      601 ataacgttga cacctctgac agaataaccg tttctgctta taatgaaaaa aaacctggct
      661 gctatggcca atgtcttgta atacaggatg acgaaacaaa cttatttaac ctgataaaaa
      721 ttaaaatttt cgaagccagg cataattatg tcgctcaatt gatgagcgat ccggaattta
      781 tgttcaaatt tagttatagc gctcaacaaa atagattaga acctcatctt ctggttccca
      841 cttatttccc tctaaaaaca aacgaaccgg ttacgcaaga ggatatactg ttactttatc
      901 gtttctttaa aatggatggt gattttaata tattaacatc agatgaatac atgagtatca
      961 tgacacctct gatgaagtgt gagcgcagca tccacgacca caacaggtat gtgacgggca
     1021 aagataccct tcttctggac tatcctcctt ctggtaacca gatatatttc catatttttc
     1081 ctgacgaatc agcaacacta gtcctttatt tttcaaacaa tgacattgaa tgtttcgtct
     1141 ttgaaagaga tattccgagc cagtatcgct ttttcaaaat gtttaccaat ctggcactgg
     1201 ttattgatca gttaaccgat gccaatcaaa tactttaatt tttatatatc ccctgtggca
     1261 aatattgcta aacaatgccc ggggattaat ttttattact cacggaaaga tttaaataat
     1321 gataccatct cagatctctt tcaatactct tcctgtcaat gcaacatacg cttcagagcc
     1381 aacagttgac acacaaaaat tctctgacat cctctactca gcggggtgtt ccctaaagga
     1441 tctggcttat ctgagcagat gtctggcatc aatacatccg actatcgcca aaaacattta
     1501 tgaaacagaa aatctcagcg atcaaaaatt actacatatt gattgccgta gtacaaatga
     1561 aatcaagata aatatctttt ttggtcaaca acgagaagga ttaattgaaa ttaattctga
     1621 cacagtcatt ttttcgatat tgtcttcatt aattgactcc gccatttcta aatttcaaca
     1681 ccaaatcccc gttaacggtt cagttaatcg tgaactattg tatgaggatt atactgatta
     1741 aatccaccct gtcgcgaaaa atggcctgcg ttattgccag acgtaataaa acctttgatt
     1801 aacgttactc atactgatag caccttcatg tacctctctt ttttacgaaa aatcaaaaga
     1861 gctaactaag gtcataattc gcatgaatca ttaaccattt gttattttca acattatttt
     1921 tacattaagc agagatgaat tgcagtgaaa ttcgacttaa atcttatgaa atgcatttac
     1981 tcatggctaa aactacgtca caaaaatgca aattagaagt agatcacaat tatctcaata
     2041 cattaaatca agttaatttt aaaaatttaa cactcatgac attagttgac ataacacact
     2101 gaattaacat ggaaatttaa aaacacactt taaacaatca tttcccgaaa gacattttga
     2161 aaatagatca attgcaacat tacggtcaga taaagacact cctgtgatat taaacctgtg
     2221 ccgttatgaa cggccataaa tacgacgtgt gtaacttatt ttactctctg tcttacaaag
     2281 gattacccat gaagaaagca acacttgtga ttggcgttat tggcgcggac tgccatgcag
     2341 taggcaataa agttctggac cgcgttttta gcaaccacga cttcagagtc attaatctgg
     2401 gcgtaatggt cagccaggac gaatatattg atgctgccat tgaaactggc gctgacgcga
     2461 ttgtcgtctc ctctatctat gggcatggcg atatcgactg tctgggcatg cgcgaacgct
     2521 gcatcgagcg tggactgggg gacatcctgc tctatgtggg cggtaacctg gtggtaggaa
     2581 aacatgactt cgctgacgtg gaaaacaagt tcaaagagat gggatttgat cgcgtatttg
     2641 ccccaagtca tgacttagaa gaggtttgtc agctgatggc ccacgatatc aaccaacgtc
     2701 atgacgttga tactcgtatt cttgaagagg ctatctgatg caaatcgtct ctgtcgatat
     2761 tggatcgacg tggaccaaag cagccctctt tacccgggag ggggacgcgt taacgttggt
     2821 taaccacgta ctgaccccaa ccaccaccca tcatctggcg aaggggtttt tctccagcct
     2881 gaaccaggtg ttaaacgtag ataatgccct ccccctgtta aacagcggtg aggttgcatt
     2941 gaagtattcc tcctctgcca aaggtgggct ggcggttgcc gcaatgggat tggtgccttc
     3001 tatcaccctg gaaaccgcga aagtaaccgc tcactctgcg ggtgctaaaa ttgcgcaata
     3061 ttacgcctac aagctgaacc gccgtgatat ccaggcactg gaagaaacgc agcccgatat
     3121 tctcttgttc accggcggca ccgacggcgg agaagaaagc tacggactga ataacgcccg
     3181 tgtgctggca gaatccaaac tcgactgcgc cattatttat gccggaaacc gggatattca
     3241 ggacgaggtg caggagatcc tcgggcataa agacctgacc atcgtcgaca acgttctgcc
     3301 cgatcttgac catccgaacc ctttagccgc acgccaggct atttgcgaca tattccttaa
     3361 acgcatcgtt aaagggaaag ggctggatgt cattgttgat aaaacaggcg aagaaccaat
     3421 gccgacacca tggacggtct ttgaactggt caaggcaatc agtaacgtcg atcacagctg
     3481 gaaagagttc atgctgatcg acatgggcgg cgcaaccacc gatgtttatt ccgcctgtgc
     3541 caacacgctc tcgcccgaca ccgttttaca cggcgtaccg gaacccttcg taaaacggac
     3601 ggtggaaggc gatctgggaa tgcgcgtctc ggcagtggtg gttggtgaaa gtgcgaaaga
     3661 gttagtcaat gtgaattttg ctcagcaacc cgcacagctg gacgcttttt atcattacct
     3721 ccgtcatcta gtcgctcatc ccgactactt acccgcaaat gcagaagaga aatatttcga
     3781 taccgtcctg gccggactgt gtgtgggtta tgccaccgaa agacatgcgg gcaccaaaaa
     3841 agaggtctgc acctgcgtcg gcaatgtcga tttacagatg ggacgtgacc tgacaaccgt
     3901 gcgtaaagtc gtgggttccg gtggatggct ctcgcgtgcc agtcagttcg atatgcatca
     3961 ctggttgaaa taccgcgagt taaacgacga cggtaaacgc attctcttac cgaccgaatt
     4021 tgaatattac cgcgattccc gggggctatt accactgctg gcaaacgtag ccagagtcga
     4081 tccgctggcc gccgcgcgca ccagtattca atgcttaacc ctataacaat taaggctgca
     4141 atcaatggaa cttcgaaata agaaattaac ccatgacgaa ttcatgaccg agcggcaaca
     4201 ggtactgaaa acctgggaaa ccggcaagga tgttgaaaac tttgaagatg gcgttaaata
     4261 ccaacaaacc atccctgagc ataaacgctt ctctcttgcg ctgctgaaag cagacaagga
     4321 aggtaaaacc cttagccaac cgcgcgcagg tgtggcgctg atggacgaac acatcgaact
     4381 gctgaaaaca ctacaagaag agtgcgatct gctgccaagc accatcgatg catatacccg
     4441 tctgaatcgg tacgaagaag cggctgtagg gattaaaaaa tccatcgaag ccggaacctc
     4501 taaactgaac ggtttaccgg tagttaacca tggtgtcgca gcttgccgtc gcctcaccga
     4561 aaccctgcaa aaaccgctac agatccgcca cggcacgcca gatgcgcgtc tgctggctga
     4621 aatctcgatg gccagtggct ttaccagtta tgaaggcggt ggcatttcct acaacattcc
     4681 gtatgccaaa cgcgtaacgc tggaaaaatc gatccgcgac tggcagtact gcgaccgttt
     4741 aatggggatg tatgaagaac acgggatccg catcaaccgc gaaccgtttg gcccactaac
     4801 gggtacgctg atcccgccgt ttatctcgca ctctatcgcg ataatcgaag gcttgctggc
     4861 actggaacag ggtgtgaaat ctatcaccgt tggttatggc caggtcggca gcctgacgca
     4921 ggatgttgcg gcgatccagt ctctgcgtga acttgcacac gaatattttc agagctatgg
     4981 ctataccgac tatgaattga gtaccgtgtt tcaccagtgg atgggcggct ttccggaaga
     5041 cgaatcgaaa gcgttcgcta ttatctcctg gggcgcagcc gtcgcgggga tgtctggtgc
     5101 caccaaagtc atcaccaaaa gtccgcacga agcctggggc atcccgactg ccgctgccaa
     5161 cattcagggg ctgaaagcct cccgccagat gctcaacatg gtcaatgagc agaaattccc
     5221 gccgtgccct gcggttgaac tggaaattga actgattaag agcgaggttc gcgcagtact
     5281 gaataaagtg ttcgaactgg gtaatggtga tattgcgcgc ggaacggtgc tggcatttga
     5341 agcgggcgta ctggatgtgc cgtttgcgcc agccgcctgc aacgcgggca aaattcttcc
     5401 ggtccgtgac aacgccggcg cgatacgcgt actggaagcc ggagccgttc cattgccaaa
     5461 agatattctc gacctccacc acgattacgt tgcggaacgc gcacgctgtg aaggacgcca
     5521 accgacattc cagatggttg tagatgacat caatgctgta tcccacagta aattaatagg
     5581 aagaccataa tgaaaataaa acaggccctg ttcaccgctg gctattcctc attctatttc
     5641 gatgaccagc aggcgataaa aaacggagcg ggtcacgacg gcttttttta taccggggag
     5701 ccagtaacac aggggtttaa caccgtacgt caggccgggg agtgcgtatc ggtacagttg
     5761 attctggaaa acggcgcggt cgctgtaggt gactgtgctg ccgtacagta ttctggggca
     5821 ggtggtcgcg atccactgtt cctcgcagag cactttattc cgttcctcaa cgaccatatc
     5881 aagccattac tggtaggccg cgatgtggat gctttcctgc cgaatgcccg tttcttcgac
     5941 aaattgcgta ttgacggcaa cttgctgcat accgccgtgc gctacggatt atcacaggcg
     6001 ctgcttgatg ctaccgcgct ggcaaccggc cgtctgaaaa ctgaagtggt ctgtgatgaa
     6061 tggcagttgc cacgcgtggc ggaatccatt ccattatttg gtcagagcgg cgacgatcga
     6121 tatatcgccg tcgataagat gatcctcaag ggcatcgacg tgctgcccca tgcgctgatt
     6181 aataacgtcg aagagaagct gggctttaaa ggtgaaaaac tgcgcgaata tgtccgctgg
     6241 ttgtcggatc gcattctaag caagcgcacc agcgcacgct accaccctac cctgcacatc
     6301 gatgtatacg gcactatcgg tctgatcttc gatatggatc cgcttcgctg tgcgcaatac
     6361 atcgccagcc tggaaaaaga agcgcaaggc ctgccgctct acattgaagg gccggtcgat
     6421 gccggtaaca agcccgatca aattcgcctg ctgaccgcga ttaccaaaga gctgacgcgc
     6481 ctcggttccg gcgtgaaaat tgtggccgat gaatggtgta acacctacca ggatattgtt
     6541 gatttcactg atgccgccag ttgtcacatg gtgcaaatca aaaccccgga tctgggcagt
     6601 attcacaaca tcgtcgatgc ggttctttac tgcaacagcc acagcatgga agcgtaccag
     6661 ggcggcacct gcaatgaaac tgatgtcagt gcccgcacct gtgtccacgt cgcccttgcc
     6721 gctcgcccta tgcgtatgct ggtaaagcca gggatgggct ttgacgaagg cctcgatatc
     6781 gtcttcaacg aaatgaatcg tactatcgcg ctgttgcagg ctaaggatta acaaatggct
     6841 cgttctttta aagtcttatc gccaacagca atcctcgggt atggattccc ggaagagagc
     6901 ttccgcaaag cgatggcaga atcacccgat ctgatcgccg ttgatgcagg ctcttccgat
     6961 ccaggccccc actacctcgg ggcaggcaaa ccgtttaccg atcgcgcagg ggtcaagcgc
     7021 gatctgcgct atatgatcac cgcaggcgtg caaaataata taccggtggt gatcggaacg
     7081 gccggcggtt ctggtgccgc tccacacctg gagtggtgcc gagaaataat tcatgagatt
     7141 gcccaggaag agcacctctc gttctcgatg gcgttgatcc cggcagacgt tgacaaagcc
     7201 atcgtccacc aggcgctgga taacggcaaa atcacggcgc tggattttgt cccgccgtta
     7261 acccacgacg cgattgacga aagtacgtat atcgtcgcgc aaatgggcat cgaacccttc
     7321 cagcgggcgc tgaaagaagg cgcgcaagtg gtgctgggcg ggcgcgctta cgacccggcc
     7381 tgctttgctg cgctcccgat catgcaaggc tttgatgaag gtctggcgct gcattgtggg
     7441 aaaatccttg aatgcgcggc aatcgccgca acgcccggct caggctctga ctgtgcgatg
     7501 ggcatcattg atgacaacgg ctttacgctg aagacattta atccgaagcg taaatttacc
     7561 gaaacgtcag cagctgcaca cacactgtat gagaaatccg atccctactt cctgccagga
     7621 cctggcggcg tgttgaacct gaaaggctgc acatttaaag cagtcaatga cggcgaagtc
     7681 tacgtcagcg gttctaagca tgaagaaacg ccgtatgccc tgaaactgga aggtgcacga
     7741 cgggtgggct tccgctgtct gaccatcgcc ggaacgcgcg acccgattat gatcgccgag
     7801 atcgataaaa tcatcgatga agtcaaaacc agcgtttcgc gtaacctgtc gctcgacgat
     7861 gacagcattc gcatcaattt ccacctgtac ggcaaaaacg gggtgatggg agaccatgaa
     7921 ccgatgcaaa ctgccgggca tgagctgggg attgtccttg atgtagttgc accgacccag
     7981 gaaattgcca atagcgtttg ctcgctggtg cgctctacca tgctgcacta cggctatgaa
     8041 aaccgcatcg ctaccgcagg caatctggcg ttcccgttct ccccttctga tatccagggc
     8101 gggccggtat acgaattttc catatatcac ctgattgaag ccaacgacgc tctgcgtttt
     8161 gatttccata ttgaacaggt gacgccagaa ggagttcagg catgaaacag tcaattgtct
     8221 cactggcgca agtcattcgt tcgaaaaatg ccggacctta cgaactggtt ttagatattt
     8281 tatttaaaac gaaagaaaac tatgagcgag ttaaatcatc agggcaatta accccagaac
     8341 tgattgcgcg cttatatcat gttgaacctg actttattca tcgtattgtc tggtttgatc
     8401 cttctaacgc agtaaaaatc gtcatgccac gagatattat ctccgggaat gtaggtgaca
     8461 atgatgtcta tggtgcacag caacatgcac ctttattaaa tatgagtttc gatttgtaaa
     8521 aatccgaata cttttagcca actgacatcc cgtcacttat tgcagttcaa cacttcagct
     8581 atttgtaagg ttgtaatagc aggtagatgc ctgaatctct attcagggat ctacttctcc
     8641 tgattctgat ttatcgccgt gcacctgatg gaggaaaaaa tgaagaaaat aagtttaacc
     8701 acgatgattc ttttggcgct ggtacttgga atgattatcg gcgtagtgct caataacact
     8761 gcttcaccgg aaacagcaaa actctatgcg caagaaatat cgatattcac gacgattttc
     8821 ttacgactga taaaaatgat tatcgctccg ttagtggttt ctaccctggt ggtaggtatt
     8881 gctaaaatgg gcgatgccaa agcccttggt cgtatttttt ctaaaacact ctttttattt
     8941 atttgcgcct cattgctgtc aattgcctta ggcttgataa cagtaaattt cttcatgcca
     9001 ggtacaggaa ttaattttgt tgcacacggt gccgaaacca ccggagtggt cgcggcagaa
     9061 cccttcacgc taaaagtatt tatttcgcat gctttcccca ccagcattgt cgatgccatg
     9121 gcgcacaatg aaattttgca aatcgtggtg ttctcaattt tcctcggctg tagcctgacg
     9181 gcgattggcg agaaaggcag cgccatcgtt cacgccttag attcgctggc acatgccatg
     9241 ttaaagctca ctggctacgt catgctcttc gctcccctga ccgtattcgc tgctatttca
     9301 gcattgattg ctgaacgagg actggcagtt atggtgagcg ccgggatctt tatgggtgaa
     9361 ttttatttca ccatgttatt actttgggtg ctgcttatcg gtctggccat cgtttatgtc
     9421 ggcccctgca tcagacgcct gacccgcgcc ctttcggaac ccgccctgct ggcatttacc
     9481 acatccagtt ctgaagcggc ttttccggga acgcttgaaa aactggagca atttggcgtt
     9541 tcccccaaaa ttgccagctt tgtcttaccc attggctact catttaatct cgttggatca
     9601 atggcctact gctccttcgc cacagttttc atcgcccagg cctgcaatat ccatttatcc
     9661 atcggtgagc aaatcaccat gctgttgatc ctgatgttga cctcgaaagg aatggctggc
     9721 gtaccacgcg cctcaatggt ggttatcgcc gccacgctca accagttcaa tattccggaa
     9781 gcggggctga tcttgctgat gggcgttgat ccgttccttg atatggggcg ttccgcgaca
     9841 aacgtcatga gcaacgcaat gggcgctgcg atggtgagtc ggtgggaagg cgaacatttc
     9901 ggcgagggct gtcggggtaa agcattaaaa cccaatgaat cgaacgttgc tctgccctga
     9961 gggggctctt aagcaatctg aagccaatga ctacgcattt ttataagccg gatcatgaag
    10021 ttagacggca attaagtaac actaacgttt aaggtgaaaa gtgaaatatg tccaaaccat
    10081 ttatctggca agaaccgttt ctacaaaata aagacggtac agaatataca ttaattagcg
    10141 atcagcatat tacagtaacg gaattagacg gcgaagaagt gataaaaatc gcccctgaag
    10201 cattaacgct gcttgcccgg caggcatttt atgaagcctc ttttttctta cgttccgctc
    10261 atttgcaaca ggtcgccagc attcttaacg atccgcaggc cagtagtaat gataaatacg
    10321 ttgcgttgca gttattacgt aatgctgaag tctcagcaaa aggggtatta cccaactgtc
    10381 aggataccgg caccgcaact attattgcca gtaaagggca acagatatgg accggaggta
    10441 acgacgccga agccttaagc aaaggcattt acaccacttt tcaggaaaat aatctgcgct
    10501 tttcacaaaa tgcgccgctg gatatgtata ccgaagtcaa tacgcaaacc aatctcccgg
    10561 cgcagattga tatctgcgcc gtcgctggtg atgagtacca tttcctctgt gttaataaag
    10621 gaggcggctc tgccaataaa gccgcgcttt atcaggaaac caaatcctta ttgcaaccag
    10681 aaaaactgac cgcattttta atcgaaaaaa tgaaatcgct gggcaccgct gcctgcccgc
    10741 cttaccacat cgcctttgtc gtcggagggc tttcagccga tcaaacctta aaaattgcca
    10801 agctggcctc aacgaaatat tacgacaatt tgcccaccag cggaaacgag cagggacaag
    10861 cattccgtga tattgagctg gaaaaagcgt tgctcgaggc cagtcagcaa tttggtattg
    10921 gtgcacaatt tggggggaaa tatttcgctc atgatattcg cgtcattcgt ctgccgcgtc
    10981 acggtggctc ttgcccgata gctatggcgc tctcctgttc tgccgatcgc aatatcaaag
    11041 caaaaattaa caaacacggt atctggctgg aaaaactcga acacaatccg gggcaatata
    11101 ttccagcctc attgcgggaa gaaaatcacg cgcaacatgt acaactggat ctaaaccgtc
    11161 cgttgcgtga cgtgatgctg gatctcgcca gattaccggt aggcacccgc gtgtcgttaa
    11221 gtggtcccat cgtagtggcg cgcgatatcg cccacgccaa aataaaagcg cggctggata
    11281 gcggcgaatc gatgccggag tacctgaagc atcatattgt ctattacgct ggcccggcga
    11341 aaacaccgga gaacatggcc tgcggttcac tgggtccaac cacgggcggt cgaatggacg
    11401 gttatgtcga taccttccag gcggccggcg gcagtctggt gatgttatcg aaaggtaacc
    11461 gcagccagca ggtcaccgat gcctgtcaca aacatggcgg cttcaatctg ggaagtattg
    11521 gcggcgcagc tgcgttactg gcgcaagaat atgtcaaaag tctgcgttgc ctggaatacc
    11581 cggagctggg tatggaagcg gtgtggatga tggaagtgga aaatctgccc gcattcatcc
    11641 tggtagatga taaaggcaat aatttcttta gtcagtttga gcagcaacac cgctgcgcca
    11701 gttgccctgc gggacattaa ggaaaatgaa tggaagcgcg aatttcaact gaacggcatg
    11761 tgcagcgcca acagaaattg aaggaacagg tcgatacgcg cgtcgcagcg gcgatagaga
    11821 aaaaagggat cttgattgtt ttcaccggca acggcaaagg gaaatccact gccgcgtttg
    11881 gtactgtgac gcgcgcggtg ggtcatggca aaacggtggg ggttgcgcag tacatcaagg
    11941 ggcaatggga caacggcgaa tacaaccttc tccagccgct gggggttgag tttcatatta
    12001 tgggaaccgg ttttacctgg gagacacaga accggcaagc ggatatcgac gcggcgaaag
    12061 aggtctggtc ggaaagcaaa cggatgctgg ctgacaagcg ttacgactta gtggtgcttg
    12121 atgagctgac ctatatgctg gcttaccatt atctggatac cgaagaagtg atcgcttctc
    12181 tgcaaaaccg cccggcacaa caaagtgtca ttgtcaccgg acgcggttgc cacagtcaga
    12241 tattaaaaat ggcagatacg gtcagcgaaa tccgccccgt caaacatgct tttgataacg
    12301 gcatacaggc ccagccgggt atcgactggt aataactgcg cctggcatcc cgccaggcgc
    12361 aggttttaca tctcctgcgg tacgaccgat ttcgccagca accattcacg aaattgctgc
    12421 aagtgcggtg agtcagactt ctcttcctgc cacgtcataa taaaacgatt accggtacga
    12481 ataggcacat cacaggggat caccatatcg ccactatcca aatcatgttg aatagcgaaa
    12541 cgtggtaaca gcgccacgcc gagattagag cgcacggcgg caatcagcat tgaaagcaaa
    12601 tcgaaacgtg gtccgttatt gaccaacgga ctgctaacgc cagataaagc aaaccactct
    12661 tcccagccag taatacgggt gctttgatgc agaagcggta actcagtaag cagttctgcc
    12721 actgacagct tttgatcgga cattgcaagc aaactgccgc tgcataccgg caaaatctcc
    12781 tcatcaaaga gatattctac ttccgcccag ggcgcgcaaa aattttcgcg cataatgaca
    12841 gcatcgtact cccgattaag aaaatcgccg ttattagcta aagaatgaat attaacaata
    12901 atatcgggat gtaactttgt gaattcatgt aaatttggga tcaaccagtg ggtactaaaa
    12961 gtaggattaa cggcaagttc aataacctga accgtaggct gccaggtcat aatggtattg
    13021 gtatcgcgtt ccagcttatt taatgtttct tttactatcc ccaggtaata cttccctgca
    13081 tcattaagaa aaatgcgttt tttagagtgt gaaaataacg gagtacgtaa aaattcctcc
    13141 agcgcactta cttgtctgaa tactgcactt tgggttaacg ctaactcctc tgccgctctg
    13201 gtataacttt catgacgtgc gacgacctca aaagtcacca gcagttccgt tttaggtatt
    13261 ttccctcgca taatgccgtc ccttaaaaat agtttaaacc atcaaaaata cattacttat
    13321 tacgaagtaa tggtaaccaa agcagcaata aataatttat aagaatcatg attgacattt
    13381 ataaa
//
LOCUS       DAZGCQ010000130        13270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000130 DAZGCQ010000000
VERSION     DAZGCQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.130"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..257)
                     /locus_tag="ACHK07_004272"
     CDS             complement(<1..257)
                     /locus_tag="ACHK07_004272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104531.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQVFHWLIYSH"
     gene            194..1693
                     /gene="pitB"
                     /locus_tag="ACHK07_004273"
     CDS             194..1693
                     /gene="pitB"
                     /locus_tag="ACHK07_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO8104532.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1742..2434)
                     /gene="yghT"
                     /locus_tag="ACHK07_004274"
     CDS             complement(1742..2434)
                     /gene="yghT"
                     /locus_tag="ACHK07_004274"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8104533.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVVKLPLMGKSLNKSIERNKVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACMRKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2608..3321
                     /gene="yghS"
                     /locus_tag="ACHK07_004275"
     CDS             2608..3321
                     /gene="yghS"
                     /locus_tag="ACHK07_004275"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8104534.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPIIGASFGRYLRGKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3353..4111
                     /locus_tag="ACHK07_004276"
     CDS             3353..4111
                     /locus_tag="ACHK07_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104535.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4157..5506
                     /locus_tag="ACHK07_004277"
     CDS             4157..5506
                     /locus_tag="ACHK07_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO8104536.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5506..6330
                     /locus_tag="ACHK07_004278"
     CDS             5506..6330
                     /locus_tag="ACHK07_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104537.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQARFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6342..6902
                     /locus_tag="ACHK07_004279"
     CDS             6342..6902
                     /locus_tag="ACHK07_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8104538.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLDKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6933..8003
                     /gene="lptF"
                     /locus_tag="ACHK07_004280"
     CDS             6933..8003
                     /gene="lptF"
                     /locus_tag="ACHK07_004280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8104539.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTDNRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWIVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            8000..9079
                     /gene="lptG"
                     /locus_tag="ACHK07_004281"
     CDS             8000..9079
                     /gene="lptG"
                     /locus_tag="ACHK07_004281"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8104540.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSLGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQAMQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKMWILHGVNHKKWLNGKETL
                     DTSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQY"
     gene            complement(9114..10286)
                     /locus_tag="ACHK07_004282"
     CDS             complement(9114..10286)
                     /locus_tag="ACHK07_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8104541.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10286..10534)
                     /locus_tag="ACHK07_004283"
     CDS             complement(10286..10534)
                     /locus_tag="ACHK07_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8104542.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10566..11480)
                     /locus_tag="ACHK07_004284"
     CDS             complement(10566..11480)
                     /locus_tag="ACHK07_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8104543.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGIAGGYDW
                     QRVQQLAAEARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11477..13165)
                     /locus_tag="ACHK07_004285"
     CDS             complement(11477..13165)
                     /locus_tag="ACHK07_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO8104544.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHTWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3192 a         3271 c         3448 g         3359 t
ORIGIN      
        1 atatgtgaat atattaacca gtggaatacc tgtgttttga ttatttctaa aggttttgaa
       61 tgaatgctta ttgtctgata cacaagaaat aacactcttt ttatcgttaa aaaatgatat
      121 ttcactttgc ccatgccgtt aaagtcactg ataatgcgtc cgttcgtaaa ttcaaaatgg
      181 cgtaatctaa tatatgctaa atttatttgt tggccttgat atatacacag ggcttttgtt
      241 attacttgct ctggcatttg tgttgttcta cgaagcaatc aatggttttc atgacacggc
      301 gaatgcggtg gcaaccgtta tttatactcg tgccatgcaa ccacaacttg ctgtggtgat
      361 ggcggcattt tttaactttt ttggcgtgtt attgggcgga cttagcgttg cctatgccat
      421 tgtccatatg ttaccaaccg atttgttgct gaatatgggg tcaacccacg gtctggcgat
      481 ggtcttttcc atgctgctag cggcgattat ctggaacctg ggaacgtggt tctttggttt
      541 accggcctcc agttcgcata ccttgattgg cgcgattatc ggcatcggtt taaccaacgc
      601 gctgttaacc ggctcatcgg tgatggatgc gttaaacctg cgtgaagtga ccaaaatttt
      661 ctcctcgctg attgtttccc ctatcgtcgg cctggtcatt gcgggaggcc tgatattcct
      721 gctgcgacgc tactggagcg gaacgaaaaa gcgtgaccgt attcaccgca ttccggaaga
      781 tcgcaaaaag aaaaaaggca aacgtaagcc gccattctgg acgcgtattg cgctgattgt
      841 ttccgctgcg ggcgtggcgt tttcacacgg cgctaacgac gggcagaaag ggatcggcct
      901 ggtgatgctg gtactggtgg ggattgcccc tgctggcttc gtcgtcaata tgaacgcgtc
      961 cggctatgaa attacccgta cccgcgatgc cgtcaccaac ttcgaacact acctgcaaca
     1021 gcatcctgaa ctgccgcaga agttgattac gatggaacct ccattgcctg cggcatctac
     1081 tgatggcgcg caagtgacgg agtttcactg tcatccggca aatacctttg atgcgattgc
     1141 gcgcgttaaa acgatgctgc caggcaatat ggaaagttac gagccgttaa gcgtgagtca
     1201 gcgcagccag ttgcgccgca ttatgctgtg catctctgat acctccgcga agctggcgaa
     1261 actgccaggc gtcagtaaag aagaccagaa cctgctgaaa aaacttcgca gcgatatgtt
     1321 aagcaccatt gagtacgctc cggtgtggat catcatggcg gtagcactgg cgctcggcat
     1381 tggcaccatg attggctggc gtcgtgtagc gatgaccatc ggtgagaaga ttggtaagcg
     1441 cggcatgacg tatgcgcaag gcatggcggc acaaatgacg gcggcagtgt ctatcggtct
     1501 tgccagttat attgggatgc ccgtctccac aacacacgtc ctctcgtctg cagttgcagg
     1561 gacgatggtg gtggacggcg gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc
     1621 gtgggtattt actttaccgg cggcgatttt tctttctggt gggctgtact ggatagcatt
     1681 gcagttgatt taaccttcct gaaaatgccc ggtcctggcg atcgggcatt tccatttttg
     1741 gctagtgata accacgcgcg gtcataaaat ccgtaatcgc tttttctgca tcaaccagca
     1801 cctgttccag tggctgattg gcatcgatat caaccagttg tgcaccacca aaggttaact
     1861 gtggcgttat ggcaatcttc ctcgccagcg attcccgttt atggtcgggt ttacgcatgc
     1921 aggcaacttc aaggtcaaca ttgagtttga tgaccagatc aggcttatgg ctcgccatcc
     1981 agtgaaacgc tttacgttcc tggcttgcca gccatgagac aaaacgacca ccttcaacgt
     2041 taggtgggaa caccgtacca tcgtaagcgc caggaatttg gtcctgagga taacggtcgg
     2101 ttagaacaat taacccgcga cgacgacagg caagcatatg acgaaagcgc agtaagcgac
     2161 gggcgacaaa cgctgtaatt accagcgccg gaactggtcc tggcaatttt tttgctgttt
     2221 tcactttatt gcgttcaata gatttattaa gcgattttcc cattaagggc agttttacca
     2281 ctgcacgacc gacatttccg gcttgttttc cgagatgaac tctttctgcg gcgccatatt
     2341 tttcgacaac ggtaatgaga tgttcacaca ccgttgactt gcctgaacca tcgctaccaa
     2401 taacggctat taatggaggt gttattgact gcatgaatat atccttaaat ttaaatatcc
     2461 atccaaaata tttatctggt taatatgttt ttatgaaagc gtaattcagg tcaatgtcac
     2521 aattaaccat tgtcacaata aggttgaacg gatattctga gcacggacca cgtaatattc
     2581 aatacattat ttgctgccgg gtttttcgtg aatcaacgta atatgtcaat aattaattcc
     2641 acgccagtgc gtgttattgc cattgtagga tgtgatggtt caggtaaatc gaccctcacg
     2701 gcaagcctgg taaatgaact ggcagcaaga atgccaacag aacacattta tctcgggcaa
     2761 tcgtccgggc gaattggcga atggatttca cagctgccca ttattggcgc atcttttggg
     2821 cgttatctgc ggggtaaggc agctcatgtg catgaaaaac cctcaacacc gcctggcaat
     2881 attactgctc tggttatcta tctgctttcc tgctggcggg cgtacaagtt tcgcaaaatg
     2941 ttgtgtaaaa gccagcaagg ctttctgctc atcaccgacc gctacccgca ggttgaagtg
     3001 ccgggttttc gctttgatgg cccgcaattg gcaaaaacca cgggcggtaa cggttggata
     3061 aaaatgttaa ggcagcgcga gctgaagctg taccaatgga tggcgtctta tttgcccgta
     3121 ttgttgattc gtcttggcat tgatgaacaa accgcgtttg cgcgtaaacc tgaccaccaa
     3181 ctggcagcgt tacaggaaaa aatcgccgtt acgccgcaac tgacattcaa tggcgcaaag
     3241 atccttgaac tggatggtcg gcaaccagcc gatgaaattc tgcaagcgtc actacgcgca
     3301 attcacgccg cgctttcctg atcaccaaaa tggatagggg atgatttaac gaatggacgc
     3361 actacaaaca caaactgtta atagtacaac cgcgccgcag cccaactaca ttccggggct
     3421 gattgcggtg gtcgggtgtg atggcaccgg taaatccaca ctgaccaccg acctggtgaa
     3481 atcgctgcaa caacactggc aaaccgagcg gcgctatctg gggctgctct ccggcgaaga
     3541 cggcgacaaa atcaaacgat tgccgttggt tggcgtctgg ctggaacggc gactggcggc
     3601 caaatcctcg aaaacccaaa gcatgaaaac caaatctccg gcgctatggg cggcggtgat
     3661 tatgtactgc ttctcgctgc gaagaatggc gaatctacgc aaggttcagc gactggcgca
     3721 aagtggcgtt ctggtggtca gcgatcgctt cccgcaggct gaaatttcgg gcttttatta
     3781 tgacggaccg gggattggcg tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca
     3841 gcgcgaacgg cgtttatacc aacaaatggc gcaatatcgc ccggaattaa ttattcgcct
     3901 gggcattgat attgagactg ccatctcccg caagcctgac catgactatg ccgagctgca
     3961 ggacaaaatc ggcgtcatgt cgacgattgg ctataacggc acaaaaattc ttgagattga
     4021 ttccagagcg ccctacagcg aagtgctgga acaggcacag aaggcggttt ctctggttgc
     4081 catcgtttct gaccgccgaa gtttaactta aggctggcgg gatacttttt actatcaacg
     4141 ataaggtcag gctgaattgg cgggttttaa catcaaacat tggtttgcag atggcgcgtt
     4201 tcgcaccatt attcgcaata gcgcctggtt aggctccagt aatgtcgtga gcgccttgct
     4261 gggtctgttg gcgctctcgt gtgccggtaa agggatgacg cccgccatgt ttggcgtact
     4321 ggtgattgta caatcgtacg ccaagtcgat cagcgatttt attaagtttc agacatggca
     4381 gctggtggtt cagtacggaa caccagcatt aaccaacaat aatccgcagc aatttcgcaa
     4441 tgtcgtctca ttttccttct cgctggatat cgtcagcggc gcggtggcga ttgtcggcgg
     4501 cattgcctta ctgccattcc tttcccattc attaggtctg gatgaccaaa gcttttggct
     4561 ggcggcgctg tattgcacgc tcattccttc aatggcttcc tccacgccga ccggcattct
     4621 gcgtgcggta gatcgctttg atttgattgc tgtacagcag gcgacgaaac cttttctgcg
     4681 cgcagcgggg agcgtcgcag cctggtattt tgactttggt tttgcgggtt ttgttattgc
     4741 ctggtacgtg tcgaatctgg ttggcggcac catgtactgg tggtttgccg cgcacgaatt
     4801 acgccgcaga aatatccata acgccttcaa attgaatctg tttgagtctg cccgacacat
     4861 taaaggcgcg tggagttttg tctggtcaac caacattgcc cactccatct ggtcggcgcg
     4921 taactcgtgc agcacagtgt tggtggggat cgtgttagga cccgctgccg ccgggttatt
     4981 taaaatcgcc atgacattct tcgacgccgc cggaacgcca gcgggtttac tgggtaaaag
     5041 tttttaccca gaggtgatgc gtttagatcc gcgcaccacc agaccgtggt tgttaggcgt
     5101 gaagtccggt ttactggcgg gcggaatcgg tattctggtg gcgctcgcgg tgttgatcgt
     5161 cggcaagccg ctaatttcgc tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat
     5221 tcaggtgatg ctgggcgcaa ttgtgatctc gatgctgggc ttccctcagg agtcattgct
     5281 attgatggcg ggaaaacagc gcgcttttct cgtggcgcaa accatcgcct caatcggcta
     5341 catcgtattg ctgttcatgt tctgtcatct gtttggcgtg ctgggagcag cgtttgctta
     5401 tttcggcggt caatgtctgg atgtggtgct ttcgctaatt ccgaccttaa aggcattttt
     5461 tcagcgccat tccttgcttt ataacgcagc cggagagaaa tcctgatgat gagaaaatat
     5521 ttcccgcttg aggcgagcga gcgtctattt gttgcgattg aagaagatga tgtcgttgat
     5581 gcacaggtca gtttgccgcc gactatcgca ctaagttgta ccaccgaaat catccatgat
     5641 aattacgcgt tatgcctgca attctggctc aatggcgttg atcgtcagga attacttcgt
     5701 ttaattcgca aacaagccaa aggcgatgag ctgacgacag acgagcgtaa acagtttaaa
     5761 tacatgcgag cgcgctacaa gcatctacgt tttgcacaac ggctgtattt aaaaaagcat
     5821 caggccagat ttttattcgg caaaacgacc gtttttctgg gacgttttca ggacggattt
     5881 cgtaacggta agaaaaatat tgtgtcgtat tacggcaacc tgttacgaat ttatttaagc
     5941 tctccggtct ggtcgttggt gaactattca ttacgccata gccagttaga aagcgtaagc
     6001 ggttttattg cctatcggca aaagcaaatg cataccctga aagagattat tgccaaaccg
     6061 cgactaaccg gcagggaatt tcatgatgtg cgaaaaatca tcagccagca ggtttcttat
     6121 tacgacacct taagatcgct tgatcctgaa aataaagagg cgttacagat ttcccgtttc
     6181 ctcgccgcca ttaatggcct gatgggggat aaacacgacg acatggtggc ggatgatatg
     6241 gaaaaccgcc agtcgtatga tgcaccggtg gcgctggata gcgatattcg ccagcgctta
     6301 gagctgctga tttcgcgttt tccgctataa ccggagcaat gatgtcatct cgtttgatta
     6361 ttgcgctgat tatcatgctc ttagccccgg gtgtgcaggc acacaatttc gtcactggca
     6421 aaacggttac ccctgtttat attcaggaag gcggcgagct gcttctgaat agcgacgacg
     6481 aaattcacta ccaaaagtgg aacagcacac aattggcggg aaaagtccgc attatccagt
     6541 atatcgccgg gcgtaagtca gcgaagaaga agaactcact gttaattaaa gcggttgaag
     6601 cggcaaactt tccacaggat cgttttcagc ccaccaccat tgtgaacact gatgatgcca
     6661 tctttggtac tggctatttt gttgttggca aaatcgaaaa aaataaacgc cgctatccgt
     6721 gggcgcaatt cgttattgac ggcaatggac agggacgcgt tgcctggcgc ttgccggagc
     6781 agagttcaac cattctggtg ctggataaag ccgggcaaat tcagtgggca aaggacggca
     6841 gcctgacgcc ggaagaggta gatcatgtca ttgcgctggc gcagaaactg atcaatgaat
     6901 aaaaaattca aaggattgag ggcgttagtg agatgaagct ggttgaacac tacatcatgc
     6961 gtggtacgcg ccgtctggtg ctgattatcg tcggttttct gatctttatt ttcgccagct
     7021 actccgcaca gcgttatctg accgaagcgg caaatgggac attagcactt gatgttgtgc
     7081 tggatatcgt tttctacaaa gtgctgattg cactggagat gttgttacct gttggactgt
     7141 atgtgtcagt tggcgtgacg ctagggcaga tgtataccga ttcggaaatt accgctatct
     7201 ctgcggcggg gggcagtccg ggacgcttgt acaaagccgt tctttatctg gcgataccgc
     7261 taagtatttt tgtcaccctt ctgtcgatgt atggtcgtcc gtgggcttat gcgcagattt
     7321 atcaactgga gcaacagtcg cagtcggagt tggatgttcg ccagttgcgg gcaaagaaat
     7381 ttaacactaa cgataacgga cgaatgatcc tttcgcagac ggttgatcag gataataatc
     7441 gcctgactga cgcgctgatt tatacttcta ctgacaatcg aacccgcatt ttccgcgccc
     7501 gttcggttga tgtggttgac ccatctcccg agaaaccgac cgttatgttg cacaacggca
     7561 ctgcctatct tctcgatcat caggggcgtg acgacaacga acagatctac cgtaatctgc
     7621 aattacatct gaatccgctg gatcaaagcc ctaacgtcaa acgcaaagca aaatcggtca
     7681 cggagctggc gcgctccgcc tttcctgccg atcatgccga actgcaatgg cgacaaagcc
     7741 gtggcctgac cgcattgttg atggcgctgc tggccatttc attaagtcgg gtaaaaccgc
     7801 ggcaagggcg attttcaacg ttattgccac tgacgttgct gtttgttgcc attttttatg
     7861 gcggcgacgt ctgccgtacg ctggtggcta acggtgcgat tcccctcatt cctggtttgt
     7921 ggatagtacc cggactcatg ctaatgggcc tgctgatgct ggtcgcacgc gacttctctt
     7981 tgctgcagaa attttcccga tgaatgtttt cagtcgctat ttaatccgtc atctctttct
     8041 cggttttgcc gccgccgcag ggttattgct gccgcttttt actaccttca acctgattaa
     8101 cgaactggat gatgtcagcc tgggcggtta tcgctggact caggcggtgc tggtggtgct
     8161 gatgacctta ccgcgcacac tggtggagct ttcgccgttt atcgccttat tgggagggat
     8221 tgtcggcctg gggcagctat cgaaaaacag tgagcttacc gccattcgca gcacggggtt
     8281 ttctatcttc cgtattgcac tggtggcgct ggttgccggg atattgtgga ctgtttcgtt
     8341 aggcgcgata gatgagtggg tagcgtcgcc attgcagcaa caggcgatgc aaatcaaatc
     8401 gaccgccact gcgttggggg aggacgatga tattaccggc aatatgctgt gggccaggcg
     8461 cggtaatgaa tttgtgacgg tgaaatcgct gaacgagcag ggccagccgg tgggcgtgga
     8521 gatatttcat tatcgcgacg atctctcgct cgaatcctac atttatgcac gcagtgccac
     8581 cattaaagac gacaaaatgt ggatcctgca tggtgtgaat cataaaaaat ggcttaacgg
     8641 taaagaaacg ctggacacat cagataatct tgcctggcaa tcggccttca ccagtatgga
     8701 tcttgaagag ctatcgatgc cggggaatac tttttctgtc cgtcagctta atcattacat
     8761 ccattatttg caggaaaccg ggcaacccag cagcgaatac cgccttgcac tgtgggaaaa
     8821 actggggcaa cccatcctga ccctggcgat gattttgctg gctgtgccgt ttacctttag
     8881 cgccccgcgc tcgccaggga tgggtagccg tctcgctgta ggcgtcatcg ttggcttact
     8941 cacctggatc agctatcaaa tcatggtcaa tctgggattg ctatttgcgt taagcgcacc
     9001 tgttaccgcg ctcggtttac cgatagcgtt tgtgctggtg gcgttgagcc tggtgtattg
     9061 gtatgacaga caatattaaa cccgccgtgg cgcaacacca cggcgggctc acattactgc
     9121 ttcagagtag caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcgttt
     9181 acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gtggcaggat aagattcaca
     9241 taaacgccca gcgaaataag ctcgcgccag atgcgtaaac cgtcctcttt cgaaccgatt
     9301 attaccggca ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg
     9361 tcatacagat ggttggcatt atccatcagc ttttgccgca gctctggttg tgaggcaatg
     9421 gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata
     9481 tacgggcggc tgccgtagcg cagcacttcc atcgcttccg agcctacagc aaaaccgcca
     9541 atcgaggcca gacttttact gaaggtgcca acgataatat ccacatcatc ttcaacgccg
     9601 acagcttcag ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg
     9661 acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg
     9721 tcaccgagca tactgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg
     9781 aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc
     9841 gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaatacc
     9901 acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa
     9961 aaaacgatag cggtagggcg attgaaaaac tcggcaatct ctttctctaa cgccaggtgc
    10021 ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac
    10081 gctgcctgac cttcggcgat ggcatcatga ttaaaggtta aaccgaggta attattggtc
    10141 ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata cacttcatca
    10201 atacaggtgc caaacggtgt taacccgttg tcactaaatt gcagtcgctc gccagcaagg
    10261 cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca
    10321 ttaattgcgc aggggttttg acatccagca gaatattaat aggaatggaa atatcgaatc
    10381 tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga
    10441 cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa
    10501 ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac ctttaaacaa
    10561 gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaatta
    10621 atatcttcag aaatacggtt attacttgcc gaccagtcgg cgtgggttaa ttcacgaatt
    10681 ttcgagcggg tcagcatagg ttctttacct gccaggcgac tcaacgcggt actgatatcc
    10741 gcaaggcagg tgagtaccgg cagaggaatg ccaaccatcc gcacagaacc acaacgggct
    10801 tcggcggcaa gttgctgtac gcgttgccag tcatagccgc cagcgatgcc atcgcataat
    10861 tcataggttt gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcaaaa
    10921 tcggtgacat gcaggaaaga gagctgtgta tctggtgcac caagtcgtgg cagcaggccg
    10981 cgcagcatcc agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc
    11041 gggcgaaaaa cgcccagcgt aatttcgtca gccattgccg tcagccgttg ttcggcgacg
    11101 tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg ccgccagcga agagataaac
    11161 agaaaacgtt ggcaaaagcc gctctctttt gctgcttgca tcaggcgcag gctgccgtca
    11221 acgttacagt gggtaaaaat ctcttctttg tgtccgcgca cttgcccggc gcaatggacc
    11281 accgcgctgg ctccggcaac cagttcgcta agcgaatgtg tatcttccag cgaaccgcgc
    11341 acccaggtga gattgtcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga
    11401 aagccgcggg cgagcaggtt atcgataata tatttaccga taaacccggt agcgcccgtc
    11461 accgcgacag tttgattcat gccagggatt cctgcacatg aagactggca gcataagcct
    11521 tctgataacg ttttttcgct tccgcacggg caggcttgcc ggaggacgtt cggggaatac
    11581 tgtggggcgg caacagatcg atagccgcgg tcacgccaaa ttcgctttgg atccgtgctg
    11641 ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct gatccgacac tggatctgca
    11701 aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc gccagaatga atttccggtt
    11761 cctgttccgc tatatattca atatcctgcg gccagatatt acggccacga ataataatca
    11821 gatctttaat gcgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg
    11881 tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaagc ctggtcgcca aagtaaccgc
    11941 tcatcagact gggaccggag atgcaaatat ggcctaccac acgttccgcg accggcatac
    12001 ctgcttcatt gcggatttca atgccatgtt ctggcaacgc tttgccgcag ttgacgaaag
    12061 tcgatacggc gcgtgtctct gcacccggcg cgacggcttt accctgatat tcgaggatgt
    12121 cgcgatccac ttcgttaacc acaaccccgg aggcttcatc agagaagctg acagccagcg
    12181 cattttctgc cagtccgtag cacggcatga aagttttatt gtcaaagtta acctgacgga
    12241 aacattcagc gaattgatgg agttgttctg cggagatggg ttctgcacca ataccagcga
    12301 cgcgccagca ggaaagatcc agttcagcga gatctttttc attcacgcgg cgctggcaca
    12361 attcatagcc aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa
    12421 gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagttgcg
    12481 tggcgacggg ggtcaggaga aagcctacca gtcccatatc atggtagaaa ggcagccagg
    12541 agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg gcttatagca cgtagattag
    12601 ccatcacttc gcgatgggtg ataatgacgc cacggggaaa acgggtgctg ccggaggtgt
    12661 actggaggta ggcgatatcg ttcggaactg gacgctggag cgcaacatcg gcttccggta
    12721 atgccttaaa ccaggtgtgg cttaatacat gcaattcggg gttgttatgc gtggcggcat
    12781 tgaccagcgg caaccactca tcaccagtga taatggcagc gggctggcaa cttgccagta
    12841 aaccctgcag tttggcgctc caggaatccc gctgaccgac gcccattgga atcgccaacg
    12901 ggacggcgac taagccggca tactggcagg caaaaaaagc ctctacgaac ccgctactgg
    12961 tttcggcaat cagcgccacg cgatcgcctt ttttcaggtt cagcgataac aaccgcttcg
    13021 caccagcttc ggcacgtgct tttaacgtct gatattccag ttgatcttca agttggcaac
    13081 gtctgtcata aaaattcatt ccagcgctac tcagagcggc gtagtccaaa gcatcaacca
    13141 gcgttggaaa atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat
    13201 acaccacgaa aaaattggat atcaaaaata aaatgacaat aatgcggtgg attatatagg
    13261 tataaaagat
//
LOCUS       DAZGCQ010000131        13176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000131 DAZGCQ010000000
VERSION     DAZGCQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.131"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(69..401)
                     /gene="emrE"
                     /locus_tag="ACHK07_004286"
     CDS             complement(69..401)
                     /gene="emrE"
                     /locus_tag="ACHK07_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO8104545.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(469..771)
                     /locus_tag="ACHK07_004287"
     CDS             complement(469..771)
                     /locus_tag="ACHK07_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO8104546.1"
                     /translation="MTGKEAIIHYLGTHKKFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(768..1469)
                     /locus_tag="ACHK07_004288"
     CDS             complement(768..1469)
                     /locus_tag="ACHK07_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8104547.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGIATMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            complement(1466..2485)
                     /locus_tag="ACHK07_004289"
     CDS             complement(1466..2485)
                     /locus_tag="ACHK07_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8104548.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVISEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSDESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2482..3021)
                     /locus_tag="ACHK07_004290"
     CDS             complement(2482..3021)
                     /locus_tag="ACHK07_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO8104549.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3052..3279)
                     /locus_tag="ACHK07_004291"
     CDS             complement(3052..3279)
                     /locus_tag="ACHK07_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8104550.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            3390..4082
                     /locus_tag="ACHK07_004292"
     CDS             3390..4082
                     /locus_tag="ACHK07_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO8104551.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            4163..5224
                     /locus_tag="ACHK07_004293"
     CDS             4163..5224
                     /locus_tag="ACHK07_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8104552.1"
                     /translation="MDTLKYEKFSDFDHNDPFFDSLKKDYKEFPLWLEKKAREGESAY
                     VLYDDKHKIEGFMYLKENDDANDINPALPPGRHLKIGTFKFESKGTLRGQRFLKKAFD
                     HAFSSKSDDIYVTVFDKHVHLIKLFQTYGFYIHGEKETHNGKEFVYARSLHEPYGDIL
                     LDYPRIMTSRANKYLLAIYPEYHTRLFPDSKLVNESPDIVKDISHANSIHKIYICGMR
                     SVMGMKRGDIIVIYRTGDKKGPARYRSVASTLCVVESVKNISEFLSEDSFVDYCIRFS
                     VFSEDELRKIYKERRYPFIIRFTYNLSLPKRPNRAILIDHVGLNGSRAFRWSHFKLTN
                     EQFLKIIELGKINESFIIH"
     gene            5202..5579
                     /locus_tag="ACHK07_004294"
     CDS             5202..5579
                     /locus_tag="ACHK07_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO8104553.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            6060..6266
                     /locus_tag="ACHK07_004295"
     CDS             6060..6266
                     /locus_tag="ACHK07_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO8104554.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            6341..6637
                     /locus_tag="ACHK07_004296"
     CDS             6341..6637
                     /locus_tag="ACHK07_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO8104555.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPLHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6643..7428
                     /gene="bet"
                     /locus_tag="ACHK07_004297"
     CDS             6643..7428
                     /gene="bet"
                     /locus_tag="ACHK07_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO8104556.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7425..8105
                     /locus_tag="ACHK07_004298"
     CDS             7425..8105
                     /locus_tag="ACHK07_004298"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8104557.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVTPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8102..8260
                     /locus_tag="ACHK07_004299"
     CDS             8102..8260
                     /locus_tag="ACHK07_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO8104558.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKQEDI"
     gene            8257..9321
                     /locus_tag="ACHK07_004300"
     CDS             8257..9321
                     /locus_tag="ACHK07_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HFO8104559.1"
                     /translation="MSQVGNHSFEFPASQGVQGGTVTLFLTIPGRSLARFLASDNYGH
                     TLARSQREINPNRVRKFLNYLTNADSRNEPFIIPPLVGNCDSNIEFVPFGNTNVGIAR
                     IPLDAEIKLFDGQHRAAGIEIFCRSSPSTLMVPMMLTMNLPLKTRQQFFSDINNNVSK
                     PSATINMAYNGRDDIAQGMISFLTQHTVFADITDFEHNVVPLKSNMWVSFKALTDATS
                     KFARNGNQQLEMGYIESVWEAWITLTQIDSIRHGVHHATYKRDYIQFHGVMINAFGFA
                     VQQMMVNHSIAEITSMIEKLCATTSSAEREDFFLMDNWAGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLETT"
     gene            9475..9696
                     /locus_tag="ACHK07_004301"
     CDS             9475..9696
                     /locus_tag="ACHK07_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8104560.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(9907..10509)
                     /locus_tag="ACHK07_004302"
     CDS             complement(9907..10509)
                     /locus_tag="ACHK07_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104561.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            10752..11096
                     /locus_tag="ACHK07_004303"
     CDS             10752..11096
                     /locus_tag="ACHK07_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104562.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            11220..11420
                     /gene="torI"
                     /locus_tag="ACHK07_004304"
     CDS             11220..11420
                     /gene="torI"
                     /locus_tag="ACHK07_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO8104563.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            11664..11837
                     /locus_tag="ACHK07_004305"
                     /pseudo
     CDS             11664..11837
                     /locus_tag="ACHK07_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(11932..>13176)
                     /locus_tag="ACHK07_004306"
     CDS             complement(11932..>13176)
                     /locus_tag="ACHK07_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO8104564.1"
                     /translation="ALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLTG
                     TELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLLQ
                     SNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFFA
                     GIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRNF
                     WVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAII
                     PFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKYS
                     VANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLLF
                     GIFFLSKKREQIVMETPVPSAI"
BASE COUNT         3821 a         3093 c         2886 g         3376 t
ORIGIN      
        1 aagcataaaa cgaggctcat ataaatatac gagcctccat attttagtcg tttagaaaca
       61 aattactttt aatgtggtgt gcttcgtgac aataaattaa taaccaacac accggcacaa
      121 atcaacatca tgcctataat ggctggcagg tccagccgtt ggccgaaaaa cccccatgac
      181 agtaagctaa tcaggacaat accgactcct gaccagatag cataagcaat ccctgtagga
      241 atataagcca gcgtctgagc taataaccag aatgatgcac aataacaaat aattgtacca
      301 acagatggcc ataaccgtgt aaaaccttct gaaaacttca ttaaggttgt accaatgacc
      361 tctgcaagta ttgcaccacc aagataaata taagggttca tagcatattc tttcctgttc
      421 aaactggaga gaattgtact acagtttgaa ctcaactcac ctgtttcatc attgtgtacc
      481 cattgatgtt cttttatata ccctcaatac ccgtttcatc gcggcactct ggcgacactc
      541 cttaaaaatc agattcgtgc tcacctttcc ttcccgttct tctctggtag cgaaccggta
      601 atacaccgtt cgccagacct ttccatcaac gaccaggatt cctgcccgcg ccattttagc
      661 cgcagcctga tttatgctgg ttaccgttgc gcctgttacc gcggcaacgt cctgcgcaca
      721 gaatttctta tgagtcccca ggtaatgaat aattgcctct ttgcccgtca tacccttgct
      781 cctttcagcc caaacttagc tttgatttct gcgatcttcg ccagagcctg tgcacgattt
      841 agaggtctac cgcccatgac aggaagttgt tttactggtt caggtatagc ctcaccacgg
      901 ttaattcgtg cggtcataca ggacagttca tcggcagcct tgcgccgtaa ttccgcgtca
      961 gtcaacgcat tggcccgcat gttctggtac aagttggtaa ccaaccagta atgcgcgttc
     1021 gatttccacg gataagactc tgcatccgga tacaggccac gcttccggca atactcgtaa
     1081 accatatcaa ccagctcgct gacgtttggc agcccggcgt taacggatgc ttcttcccgg
     1141 caccaggcga caaactgccc gggtgatggc aggaatggtc gattctgccg acgggctacg
     1201 cgcattcctg cgttaacctg ttccattgtg gcgatcccgt tttcccggaa agccagcacc
     1261 cactggcggc ggatttcgtt cagttcgttc tggtcccggt tagccaggct cgccgggaaa
     1321 gttgccagta actggctgaa cacaccattg ataatctgcg ctacctgctg tacctgcggc
     1381 ttttcgtcgt actgttccgg catgttgttg gcgatccgac gcatctgctc acggtcaaag
     1441 ttaatcatct gtgcggcgat gtttttcata gatcaacccc gtaaatccag tctgtgtttg
     1501 tcaggtcgag ttttggtttg ctggctgtca cgcctgcctg ttgcttgtta cggttgattt
     1561 cgagctgggt ccacttgtcg cggagtttgg ccggacttag cacgttaccg gaccagaagt
     1621 tgtcctggca tgcccagcgg aacagcacgc acatgtcgcg gtggttacgt ccatcacgtt
     1681 cacgcatcag gcggatatcg ttagcccacc ctgcaaaatt cggttttctg gctgatggcg
     1741 cgatggtctt caccatgtca aacatccact ctgcggcggt caggtcttct gctgtccccc
     1801 acttgctgcc gctctgaatt gcagcatccg gtttcaccac aggaagatcg ttttctggct
     1861 ggtcagagga ttcgtcagaa ttctcggacg aaaaaggttt tatattgtct tttgttagtt
     1921 tgtcttttgt gtttacctga ttcgggtaaa cgcctttacc tgatttgggt aaacttttct
     1981 tacctgattc aggtaaattt acctctttca ggtaaacttt atttttctta cctgattcgg
     2041 gtaatgttga ccattcactg atcacattat taatgcctat attccgcccg ctctgaatca
     2101 aaatcccacg ctttaccaga acactttttg cagcagaaca cttgtgcggc aatatcccgg
     2161 ttaattcgga aagttgctcg ttgctaaccc aatccagttt tttattaaag ccatatgttt
     2221 tgcgcatgac agccagaaag accagaagct ggtgctgtgt taatccggcc agcatcacag
     2281 cttccagcaa ctcatttgca atgcgcgtat aaccatcatc gagatctgcc acgcgccgct
     2341 ccttttgtgc cacatccggc actggaaaat tgaatatctc agcagtgttt gccataattc
     2401 ctcccgcaat gagtgtgtta cgatttgcac ctgaaagtcg gttctgttcg cgcagaccgg
     2461 ctttcgccat ttccgaacct gtcatattgc ccccagcatg gtggtgacca tcgccatcag
     2521 tggaccagcc agatccgggt ccacacgaaa catcgacaca atgccttcac tcatctcctt
     2581 cagtttctgg tggcgtggtg cgttgagaat gacagcctgt tttgcctcac tgagttcctt
     2641 ttccatttca gccagccgag ccatgaagct atcctgctca accaggtggc cgcgatattc
     2701 cagcggtagt accgccagaa ttgccggggt cagttcacgc acgttatttc ggtatttttc
     2761 agaatcgaat ttgttatcga ggaagcggaa cagcttctgg cgtgcacggc tgacatcatc
     2821 agggaaatcg atggtgccgc cgccctgctc ccgatactca ttcacaatga gtgcggcaac
     2881 aacatcctga ttatctgcag ccgaccaggc gcggacggca tcacggattt tttcgtggcc
     2941 tggagcttgt tttgtttgag aacgatttat caccgcagtc gggctaaatc cgctagtctg
     3001 ttggtatgta agtggttgca tagtcattgc cttatcagtt aacgccgcag attaggcggc
     3061 agaattactc gcgttaaaca atggtgcgag gtcgggacga atatctgctg gtttaatctt
     3121 tccaccagtg gctgagacaa ttttcattac ataacgggca tcaattccgc caccgtgtag
     3181 ccaacgccaa acagtgggct gggctacacc gcatagatct gccagtcgtt tttgactacc
     3241 tgtaatactg attgcgagtt gaatggtttg atttgtcatt atcaattcct attggtatta
     3301 caatgaataa ataatagcaa tacgtattaa tcataacaat agcaaaacgt gttttgacca
     3361 tcaatacgca agcgtataaa ttaaaactta tgaaaaaaga aactcttgct gatcgcttaa
     3421 acctagcgat ggaacaatct ggaatgtctc aaggcgctct tgcaaaggcg tctggcgtag
     3481 ctcaacccac aatctggaga ctgacaagcg gcaacgcgcg cggctcaaca aaaattgttg
     3541 aaatagctaa tgcattgggt gttcgaacag agtggctctc atcaggcata ggcccgatga
     3601 gaaatgacgg tcaacaatta gggaagccta ctgccaacca tcccaaatac ttcaagattg
     3661 acgttcttga tatagaagtg agtgccgggc cgggagtcat caaccgtgag tttgtagaag
     3721 ttctacgctc ggttgagtac tcgtttgacg atgctcgtca catgttcgat ggtaggaagg
     3781 cagaaaatat ccgcatcatt aacgtacgcg gtgacagcat gtcaggaacg attgaacctg
     3841 gtgatcttct attcgttgat atcacggtta aatctttcga cggtgatggt atctatgcgt
     3901 ttctgtacga cgacacagcc catgtaaagc gcctgcaaat gatgaaggat aagctgctgg
     3961 ttatctctga taacaaaagc tactcaccgt gggacccgat cgagaaagat gagatgaacc
     4021 gggtatttat cttcgggaaa gttattggga gcatgccgca gacgtatagg aagcatggat
     4081 agtaccaatt aaaaattatc aaccgggcat tgtgctcatt cagtaaaaca acttaattat
     4141 tcattttaga atggagaact taatggatac tttaaaatat gagaaattct ctgactttga
     4201 tcacaatgac ccattttttg actctttaaa aaaagattat aaagagtttc ctctttggtt
     4261 agaaaaaaaa gccagagaag gagaatcagc ttatgtgctc tatgatgaca agcataaaat
     4321 cgaaggtttt atgtatctaa aagaaaatga tgatgcaaat gacattaatc cagcgctccc
     4381 accaggacgt catctaaaga taggaacatt caaatttgaa tctaaaggca cccttcgcgg
     4441 acaacgattt ctaaaaaaag cgtttgatca tgcattttca tcaaaatctg atgatattta
     4501 tgttactgtt ttcgacaaac acgtccatct aataaaactt ttccaaacgt acggatttta
     4561 cattcatggt gaaaaagaaa cacataacgg gaaagagttt gtatatgcga ggtctttgca
     4621 tgagccttat ggtgatattt tattagatta ccctcgaata atgacatcaa gggccaacaa
     4681 atatttactg gcgatttatc ccgaatatca cactagacta ttccctgatt caaaacttgt
     4741 aaatgaatca ccagatattg tcaaagatat atcccatgct aacagcattc ataaaattta
     4801 catatgtgga atgcgttctg tgatgggaat gaaaagagga gatatcattg tcatctatag
     4861 aaccggagac aaaaaagggc cagctcgcta tcgttctgta gccagtacat tatgtgtagt
     4921 tgagagcgta aaaaatattt ctgaattttt aagcgaagat agttttgtag actattgtat
     4981 tcgttttagc gtattttctg aagatgaact cagaaaaatc tataaagaac gtcgataccc
     5041 tttcattata agattcacat acaatctgtc tttgccaaag agacccaatc gtgctatttt
     5101 aatagatcat gtggggctaa atggttcgcg tgcattccga tggagtcact ttaaactcac
     5161 aaatgagcag ttcttaaaga tcatcgagtt aggcaagata aatgaaagtt ttattatcca
     5221 ttaagcctga gtttgcagaa aaaatattga acggaacaaa gcggttcgag tttcgtaaag
     5281 gtatattcaa aaatccgcaa attagcaccg ttgttattta tgccacgatg ccattaggta
     5341 aagttgttgg tcaattccgt attgaatcaa tactaagtga cgaaccggaa tctctttgga
     5401 aaaagacgga aaaacacgca ggtatttcta agcaatttta tgactcatat tattcaggta
     5461 gagaaaaggc ctacgcaata aaaattggtg aagtggaaag atataaagaa ccaattccta
     5521 tctctgctct aggtagtaat attaagccac cacaatcata tctttaccta cctgcgtaag
     5581 aatcccggcc accgtgccgg gttttctttt gtcccctccc ctcatcacac aaaccgttcg
     5641 aaaaaccacc acaacctccc ttcagttatc gctatgcgat gcaagtcaca aaattaattc
     5701 tttttgctat caaacagtta atatcaaaac acatcaatca atatcaataa gtattgatgc
     5761 aaccaatagc aatagctatt atcaccatgt cgcaacaaca caacgatacg gcaatcacct
     5821 gattcaccgt tgcgatgacc gcttagatcc gcagcttgaa tttcagcagg ctccggggag
     5881 tgcgaggggt gaagcggacg cgtgaacgtc ggtgtgacca gctgaaatca actcaacacc
     5941 tcatacctca gtcgcttcaa cgaggcggct tagttatgac aaccggcggc catccaccgc
     6001 ctgaatacgc gcagaagtct ctatatgttc agcagcccag cttacgggca ggagttttta
     6061 tggttcatca acattacgga acgcagaccg ttaatcgcgg tgcggtcatg ccaggaatgc
     6121 tggtcaaaca caaagatggt acctggactg catcagctaa tttacgcgga cggctttatc
     6181 tgcatcgcgg catcgagcgc acttataccc gtgatttgct cgtggaagtt tttctcgacg
     6241 gacgcggcaa cggcctgaat cactaatccc ctttcctgtt tacctaatca gcccggcatt
     6301 tcgcaggcga tattttcaca gctatttcag gagttcagcc atgaacgctt attacattca
     6361 ggatcgtctt gaggctcaga gctgggcgcg tcactaccag cagatcgccc gtgaagagaa
     6421 agaggcagaa ctggcagacg acatggaaaa aggcctgccc ctgcacctgt ttgaatcgct
     6481 atgcatcgat catttgcaac gccacggggc cagcaaaaaa gccattaccc gtgcgtttga
     6541 tgacgatgtt gagtttcagg agcgcatggc agaacacatc cggtacatgg ttgaaaccat
     6601 tgctcaccac caggttgata ttgattcaga ggtataaaac ggatgagtac agcactcgca
     6661 acgctggctg ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg
     6721 atcaccactc ttcgccagac agcatttaaa ggcgatgcca gcgatgcgca gttcatcgca
     6781 ttgctgatcg tcgccaacca atacggcctt aatccgtgga cgaaagaaat ttacgccttc
     6841 cctgataagc agaacggcat cgttccggtg gtgggcgttg atggctggtc ccgcatcatc
     6901 aacgaaaacc agcagtttga cggcatggac tttgaacagg acaacgaatc ctgcacatgc
     6961 cggatttacc gcaaagaccg caatcatccg atctgcgtta ccgagtggat ggatgaatgc
     7021 cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca cggggccgtg gcagtcgcat
     7081 cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctcgc cttcggattt
     7141 gctggtatct atgacaagga tgaagccgag cgcattgtcg aaaataccgc atacactgca
     7201 gaacgtcagc cggaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac
     7261 actctgctga tcgccctgga taaaacatgg gatgacgact tattgccgct ctgttcccag
     7321 atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc agtgaaagct
     7381 cttggattcc tgaaacagaa agccactgag cagaaggtgg cagcatgaca ccggacatta
     7441 tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcgtggcaca
     7501 aattacggct cggcgtcatc accgcttcag aagttcacaa cgtgatagca aagccccgct
     7561 caggaaagaa gtggcctgac atgaaaatgt cctacttcca caccctgctg gctgaggttt
     7621 gcaccggtgt gactccggaa gttaatgcta aggcgctggc ctggggaaaa cagtacgaga
     7681 acgacgccag aactctgttt gaattcactt ccggcgtgaa tgttactgaa tccccgatca
     7741 tctatcgcga cgaaagtatg cgcaccgcct gctctcccga tggtttatgc agtgacggca
     7801 atggccttga gctgaaatgc ccgtttacct cccgggattt catgaagttc cggctcggtg
     7861 gtttcgaggc cataaagtcg gcttacatgg cccaggtgca gtacagcatg tgggtgacac
     7921 gaaaagatgc ctggtacttt gccaactatg acccgcgtat gaagcgtgaa ggactgcatt
     7981 atgtcgtggt tgagcgggat gaaaagtaca tggcgagttt tgacgagatg gtgccggagt
     8041 tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc
     8101 gatgacgcat cctcacgata atatccgggt aggcgcgatc actttcgtct actccattac
     8161 aaagcgaggc tgggtatttc ccggcctttc tgttatcaga aatccactga aagcacagcg
     8221 gctggctgag aagataaata ataaacagga ggatatatga gtcaggttgg taatcattca
     8281 ttcgaatttc cggcatcgca aggtgtacag ggtggtactg ttacactctt ccttaccata
     8341 ccaggaagat cgctggctcg tttcctcgct tcagataatt acggccatac actggcacgc
     8401 tctcagcgag aaattaatcc aaatcgagta cgaaaatttt taaattatct cactaacgca
     8461 gactcaagaa atgagccttt tatcattccc cctctcgtag gtaactgtga ttcgaatata
     8521 gaatttgtac cgtttggcaa cacaaatgtt ggtatagcca gaattcccct cgacgccgaa
     8581 ataaaacttt ttgatggtca acatcgtgca gctggcattg agatattttg ccgaagttcc
     8641 ccatcaacgc tcatggttcc catgatgctt acaatgaatc tgccgctaaa aacccggcag
     8701 cagttctttt cggacataaa taacaacgtt tctaagccat cagcgaccat caatatggcg
     8761 tataacggcc gggatgatat tgctcaggga atgatatcct tcctgaccca acatactgta
     8821 tttgccgata taaccgattt tgaacacaac gtagtgccat taaaaagtaa tatgtgggtg
     8881 agtttcaagg cactcactga tgcaacgtca aagttcgcta ggaacggcaa tcaacaactt
     8941 gaaatgggat atatagaatc tgtctgggag gcatggatta cactaactca gattgactca
     9001 atccgacatg gtgtacacca cgctacgtac aagcgcgatt atattcagtt ccatggagta
     9061 atgattaacg ctttcggttt tgcggttcaa cagatgatgg ttaatcattc catcgcagaa
     9121 ataacttcta tgatcgaaaa actatgtgca actaccagct ctgcagaaag agaggatttt
     9181 tttctgatgg ataactgggc ggggatctgc acgaaagcca gccaggaaaa actatctgtt
     9241 attgccaatg tggcagcgca gaaagcagca gcaaacagac tgatacaagc ttttaccaaa
     9301 ggaagtctgg aaacaactta atgaatcaac attgtctcat atcagcatgc tgtacggcgt
     9361 ctttaaggaa cggtgagcat gaaaaacaaa atcatcatgg agctacaggc tcctttttta
     9421 ttattcgcat tcaccctcaa gcgtattaac caacaattca gggattaatg aaagatggca
     9481 gacatcattg attcagcatc agaaattgaa gaattacagc gcaacacagc aataaaaatg
     9541 cgccgcctga accaccaggc tatatctgcc actcattgtt gtgagtgtgg cgatccgata
     9601 gatgaacgaa gacgcctggc cgttcagggt tgtcggactt gtgcaagttg ccaggaggat
     9661 ctggaactta tcagtaaaca gagaggttcg aagtgagcga aattaactag aagccaaaga
     9721 taaaatcatc gctgagcagg agaaaatcgc taacggagaa aagacagtaa gtcagtatat
     9781 gaaaaccgca tgatatcatc agataaaaac cgatcgtaaa gcgaaatatt aataccagaa
     9841 taaacgagtc gaggtaaatt atattacctc gataaattaa ctaaaacttg cccgctatat
     9901 actatctcat tcagtatcat cacgcgcggt ctgtgcatat gtcactaccg cacctaatgt
     9961 attaattttc ttttcaacat agataatatt atcgtactca taattgccat acggatagca
    10021 aatgcgaata ttctcatgta gatcggggtc atccacctca gctccagaac aactttttga
    10081 actaccggaa gtataccgat acggtgcaac ataagacgat gtctctccag gcaaaaaata
    10141 agttagtgtt gtaaggggta taatcagaaa aaatccagaa aatatgcaca tccctgcata
    10201 aaccttaagg tatgctgaca gactcttcca gccgctttgt tttactatcc ccttcttaac
    10261 ccaaaacaga gataacagaa aagctattcc catgctaaac agaatgtaat agtgggatat
    10321 actctgatta agaaacgtga ccctgtagat atctgcccgc caccagaaga aaaggaaaat
    10381 aaagatcagc cctgaaactg tcatgcaaat caaataagga tacgaatctt ttttcatgtt
    10441 tagcgcccat aaaatttttc ctgcaccgga caaatttacc atccattttt tgcgcagaaa
    10501 atagctcatt acttactgca caataataca caaaattgcg taaatttttt gcatggattt
    10561 tagctctttc agccgacgtt taaggggtaa atagcatttc ctaaaagcaa ctgcaccaac
    10621 ccaacagaat gggctaccgc ttacgttgag agcaaaaaag tgtatagcag caatgaacag
    10681 catcctcgca ctgacgggga tttcttttat ctgagctcgc tacggcgagt tttgttttat
    10741 ggagacaaga aatatcagat ttggctatga aggttttgaa atggcaatca actggcgatg
    10801 tcggcatcag tagcgcaact cttgcctcaa tcgcatgtgg cctgaaaaag aatatctatg
    10861 gtcatcactt cggcgctccc catgacgcag cagactttcg gcgatgcgtt gcacttgttg
    10921 agcagattcc agaaatcaga gattcattcg acaaggttgc aaagcgcgtt ccggcattca
    10981 aaggaatcct caacgaatgg gattcactcg ttgctctgtt gaagtctgaa atgaagacgt
    11041 acgggaacaa agcaccagag acttacagaa gaatcagcga gctacgcaag gactaacccg
    11101 cctcacactc gatgaggcat tttcatttat caagatatcc agacctacca tcgccgcatc
    11161 aatgcggttt ttttattacc tgatttgcag gttcgattcc ctattcggag atagcactca
    11221 tgcaacacga actacagcct gattccctgg ttgatttgaa attcatcatg gccgatactg
    11281 gcttcggtaa aaccttcatc tatgaccgga ttaagtccgg agacctgcct aaagccaaag
    11341 ttatccacgg gcgagcaaga tggttatatc gtgaccattg tgaattcaaa aataagctct
    11401 taagccgcgc caatgggtaa aatagcgggt aaaatatttc tcacatctaa aaaacaccat
    11461 tctaatcaac cccctgccgc ctcaagtaga tgtctgcagg ggacaccaga tacccttcaa
    11521 acgaaatcta ccttcacccc gtaaaagatg ggtttggcag cacacttgcc ctatatctac
    11581 tcatttttac tgcaacaggt tgaaatctca gcactgtcag aaagcgctga tgactaaaca
    11641 gccttgggcc gggcgatgta accatcacac agaatcctga tagcgaaata tggcgtgact
    11701 cgatacttca ctccgcaatg cattccttga tgaattcgca ggcccgtgat acacgggaca
    11761 ggtcgctgaa tgacgacaat gtcctggaaa tcagcgaacc gcgtatccga agtacatttg
    11821 agcgactgta ccagaacata ggtgattatt agcagggcta agcattttag tattattatt
    11881 ttccggttga gggatatgga gatatcgaca acaaccggaa aaagtttacg tctatattgc
    11941 tgaaggtaca ggcgtttcca taactatttg ctcgcgtttt ttactcagga agaaaatgcc
    12001 aaatagcaac atcaggcaga caatacccga aattgcgaag aaaactgtct ggtagcctgc
    12061 gtggtcaaag agtatcccag tcggcgttga aagcagcaca atcccaagcg aactggcaat
    12121 ttgaaaacca atcagaaaga tcgtcgacga caggcgctta tcaaagtttg ccacgctgta
    12181 tttgaagacg gatatgacac aaagtggaac ctcaatggca tgtaacagct tcactaatga
    12241 aataatccag gggttaacga acagcgcgca ggaaaggata cgcaacgcca taatcacaac
    12301 accgataagt aatgcatttt ttggccctac ccgattcaca aagaaaggaa taatcgccat
    12361 gcacagcgct tcgagtacca cctggaatga gttgagataa ccatacaggc gcgttcctac
    12421 atcgtgtgat tcgaataaac ctgcataaaa gacaggaaaa agttgttgat caaaaatgtt
    12481 atagaaagac cacgtcccca caataaatat gacgaaaacc cagaagtttc gatccttgaa
    12541 aactgcgata aaatcctctt tttttacccc tcccgcatcc gccgctacgc actggtgatc
    12601 cttatcttta aaacacatgt tgatcatcat aaatacagcg ccaaatagcg agaccaacca
    12661 gaagttgata tggggactga tactaaaaaa tatgccggca aagaacgcgc caatagcata
    12721 gccaaaagat ccccaggcgc gcgctgttcc atattcgaaa tgaaaatttc gcgccatttt
    12781 ttcggtgaag ctgtcaagca aaccgcatcc cgccagatac cccaagccaa aaaagagcgc
    12841 ccccagaatt agacctacag aaaaattgct ttgcagtaac ggttcataaa cgtaaatcat
    12901 aaacggtccg gtcaagacca ggatgaaact catacaccag atgagtggtt tcttcagacc
    12961 gagtttatcc tgaacgatgc cgtagaacat cataaataga atgctggtaa actggttgac
    13021 cgaataaagt gtacctaatt ccgtccctgt caaccctaga tgtcctttca gccaaatagc
    13081 gtataacgac caccacagcg accaggaaat aaaaaagaga aatgagtaac tggatgcaaa
    13141 acgatagtac gcatttctga atgggatatt cagtgc
//
LOCUS       DAZGCQ010000132        13161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000132 DAZGCQ010000000
VERSION     DAZGCQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.132"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(541..966)
                     /gene="arsC"
                     /locus_tag="ACHK07_004307"
     CDS             complement(541..966)
                     /gene="arsC"
                     /locus_tag="ACHK07_004307"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO8104565.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDQL
                     VKLIADMGLTVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1299..1472)
                     /locus_tag="ACHK07_004308"
     CDS             complement(1299..1472)
                     /locus_tag="ACHK07_004308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104566.1"
                     /translation="MHPLTHPLPVTVHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENASELTG"
     gene            1500..1583
                     /gene="dinQ"
                     /locus_tag="ACHK07_004309"
     CDS             1500..1583
                     /gene="dinQ"
                     /locus_tag="ACHK07_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO8104567.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1637..2989)
                     /gene="gorA"
                     /locus_tag="ACHK07_004310"
     CDS             complement(1637..2989)
                     /gene="gorA"
                     /locus_tag="ACHK07_004310"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO8104568.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(3061..3903)
                     /gene="rlmJ"
                     /locus_tag="ACHK07_004311"
     CDS             complement(3061..3903)
                     /gene="rlmJ"
                     /locus_tag="ACHK07_004311"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO8104569.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            4106..6148
                     /gene="prlC"
                     /locus_tag="ACHK07_004312"
     CDS             4106..6148
                     /gene="prlC"
                     /locus_tag="ACHK07_004312"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO8104570.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            6156..6908
                     /gene="rsmJ"
                     /locus_tag="ACHK07_004313"
     CDS             6156..6908
                     /gene="rsmJ"
                     /locus_tag="ACHK07_004313"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO8104571.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6957..8426)
                     /gene="dtpB"
                     /locus_tag="ACHK07_004314"
     CDS             complement(6957..8426)
                     /gene="dtpB"
                     /locus_tag="ACHK07_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO8104572.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGSKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMVAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8592..8645
                     /locus_tag="ACHK07_004315"
     CDS             8592..8645
                     /locus_tag="ACHK07_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104573.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8623..8676
                     /locus_tag="ACHK07_004316"
     CDS             8623..8676
                     /locus_tag="ACHK07_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104574.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8743..9177)
                     /gene="uspA"
                     /locus_tag="ACHK07_004317"
     CDS             complement(8743..9177)
                     /gene="uspA"
                     /locus_tag="ACHK07_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO8104575.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9568..9903
                     /gene="uspB"
                     /locus_tag="ACHK07_004318"
     CDS             9568..9903
                     /gene="uspB"
                     /locus_tag="ACHK07_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO8104576.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9974..11473)
                     /gene="pitA"
                     /locus_tag="ACHK07_004319"
     CDS             complement(9974..11473)
                     /gene="pitA"
                     /locus_tag="ACHK07_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO8104577.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11705..12907
                     /gene="yhiN"
                     /locus_tag="ACHK07_004320"
     CDS             11705..12907
                     /gene="yhiN"
                     /locus_tag="ACHK07_004320"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO8104578.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         3287 a         3323 c         3478 g         3073 t
ORIGIN      
        1 taaatatcat gcgtcatatc ttcctgatat aattaccttt gacttccctt ttatctccac
       61 ccggtaatat ttgtgtataa aaaagggcat tgatagtatc gtaaaggaaa acgttaacaa
      121 ctatcaaaaa gcgctcattt taaaaagtta tgatgttctc cgatagtgtg tgaaatgata
      181 tttttgtgat gttttagatg tgtaataata aaatgggtta aggtatctta taattattga
      241 ataatttgtt gtgtaaagca ctgataaatc ataatccagt acgtctttaa actgctgcac
      301 tgtatcgcgg ataactaagc agctcactga tacaccatgc tggacgcgat aattggaatc
      361 tcacgcaggg agtaatgtgt aaatagcaaa atcgcctgat gcgctacgcc tgtcaggcct
      421 acaaataaca tactattcat cgaattttca taactttgta ggtcggataa gacgcgccag
      481 cgtcgcatcc gacataaata aagcgcactt taccaactat cggcagtgga tatcgccgct
      541 ttatttcaac cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaacgc
      601 gcctttttgc gcatctggca gaatttccag caccacttcc gaggggcggc ataggcgagt
      661 tcccagcggc gtcaccacaa tcggacggtt aatcaggata ggatgttgca gcataaaatc
      721 aattaaccga tcgtcagtaa atttatcttc tgcaaggccc agttcctcat acggctcgac
      781 gtttttacgc agcagcgcgc gtacggtaag ccccatatcg gcaatgagtt tgaccagttg
      841 atcgcgcgtt ggcggtgttt ccagataata gataatggtc ggttctgtac cgctgttgcg
      901 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      961 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
     1021 tacaaaccaa agcggataag tcaaaaacat atatggttta acgaatgcgt atgtgcagag
     1081 gaaggtaata ggtgtgaatt ttgagttggc tattcatttg aaaggaggtc cgaatcagga
     1141 cgtaaaaaaa ccgactttgc gtcggttttt tactttccag ccctgagttg gtggctctgg
     1201 ctggagtgag atagccatca tctaacgcat caatgctaat accatactga aactattgca
     1261 aggacgtgct ggttttataa cctgcatgta ctgtatgatt atccagttag ctctgaggca
     1321 ttttcactct ggcaatgcgc ataaacgctt tcaaagtcct ggtcagaagt acgggtggtg
     1381 ccgttaactg atgctctggc cggagtgaga tagtcgtcat ctaacaatga gacatgcacc
     1441 gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg agaaaacggg
     1501 tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa ctgatccgct
     1561 ttctgcttca gcttctgaac tgatagcgga aacgtaatta agggctaaga gcacactact
     1621 cttagccctt taacatttaa cgcatggtca cgaactcttc tgccgccgtt gggtgaatgg
     1681 cgacggtatt gtcgaagtct tttttggttg cccccatctt cagcgccacc gcgaagccct
     1741 gcaacatttc gtccatacca aaaccaatgc cgtgaatacc gacaatcttc tcttctggcc
     1801 caacgcacac cagcttcatg cggcacggct ggcggtgagt ggtgacggcg gtatacatcg
     1861 cggtgaaaga ggatttatac actttcacct gatcgtcgcc gtactgctcg cgcgcctgcg
     1921 gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc ggaatgttgc
     1981 tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagagg cggcgacccg
     2041 ctgcaactgc caccggtgtc agttctactg cacccgtgtt atcgcccacc gcgtaaatac
     2101 cttcaatgtt ggtgttttga tatttatcga cgacgatata gcctttttcg ttggttttaa
     2161 cgccagcggc ttcgaggttg atgttgtcat tggcaggctc gcgaccaatc gcccaaatca
     2221 ggcaatccac cgtttcactg cgaccatctt ccagctccag cgtcaggcta ccatcggcat
     2281 ttttcactac cgctttcggg atggcgttgg tgtgcaactg cgggccttcg gcgttcatca
     2341 cttcaaccag cgtttcagag atcatcgggt cgaagctgcg cagcggcgca tgtttacgca
     2401 caaacagatg cgttttcgcg cccaggccgt taatcacgcc cgccagctca acggcgatgt
     2461 aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag aagccatcag
     2521 aatcaatacc gtattccacg cccggaatat ccgggtggct cggacgaccg ccggtggcga
     2581 tcaggatatg atcggccgtg atggtttcgc cgtttacttc cagcgttttg gcatcaacga
     2641 agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt tcataggaag
     2701 tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag ttgaatttat
     2761 tgatagtggt atcaaaacca taatccgggc cgtacatatg gatcgcttca cggatttgcg
     2821 ccgcgtgcca catcactttt ttcggcacgc agccaacatt cacgcaggtg ccgcccagct
     2881 ctttggcttc aatcagcgcg catttctggc cgtacatagc cgcgcggttg atggaggcga
     2941 taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg acagagtgtc
     3001 cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca atggctgcaa
     3061 ttactccggc acgatccagc ttacggtggc gtgcccggtg ccagccggaa ccagtttgct
     3121 gtgcagccac ggcagcacgt tattcatctg ctgttccagc ttccacggcg ggttaatcac
     3181 aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagtaccg ccagttcaat
     3241 ttgcagaatt ttgcgaatac cggtcgcttc cagatcgtgg atcatgcgct taatttgctg
     3301 acgcagcacc accggatacc acagtgcgta agtaccagtg gcgaaacgtt tgtaaccttc
     3361 tgctatcccg ctgaccaccg cttgatagtc ggttttcatt tcatacggcg ggtcgataag
     3421 gattaaacca cggcgggaaa ccggcggcag tttggcctta agctgctgga aaccgtcgga
     3481 tttttcgacg cgcgcacggc tgtctttctg aaattcagaa cgcagcaacg ggtaatcgct
     3541 cgggtgcagt tcggtcagtt gcaggctgtc ctgttcacgc agtaactgac gggcaatcaa
     3601 cggtgaaccg gggtagtaac gcaactgacc gctacggttg aagtgtttta ccacattgat
     3661 gtacgcctcc agctccgcag gcaaatcgtc ctgctgccag atacgggcga taccttcgag
     3721 atattcgccg gtacgctcgg catgttcgct acctaactga taacgcccgg cgcctgcgtg
     3781 ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga tcaggctctg
     3841 aacggtatgt ttaagtacgt cggcgtggtt gccagcgtga aagctgtggc gataactgag
     3901 catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc acaaaagcct
     3961 atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt ctcattgaaa
     4021 ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga gcgcttcctt
     4081 aacctcttta accaggactg cgctaatgac gaatccgtta ctgactccct ttgaattgcc
     4141 tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg cattgaatga
     4201 ctgccgcgaa aatgtggagc gcgtagtagc gcaaggagca ccgtacacct gggaaaatct
     4261 ctgccagccg ttggcggaag tggacgatgt gttggggcgt attttctccc cggtcagcca
     4321 cctgaactcg gtgaaaaata gcccggaatt gcgcgaagcg tacgaacaaa ctctgccgct
     4381 gctgtcggaa tacagcacct gggtagggca acatgaaggg ctgtataaag cgtaccgcga
     4441 cctgcgcgat ggcgatcatt acgccacgct gaacacggct cagaaaaaag cggttgataa
     4501 cgcactgcgc gattttgaac tctcaggcat cggtctgccg aaagagaaac aacagcgtta
     4561 cggcgaaatt gccactcgtc tttctgaact gggcaaccag tacagcaaca acgtccttga
     4621 tgcgacgatg ggctggacca aactcgttac cgacgaagcg gaactggcgg ggatgccaga
     4681 aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa gagctggaag gctacctgtt
     4741 gacgctggat atcccaagct acttgccggt aatgacctac tgcgacaacc aggccttgcg
     4801 tgaagagatg tatcgtgctt acagcacccg cgcttcagat caaggtccaa acgccggtaa
     4861 atgggataac agcaaggtga tggaagagat cctcgctctg cgtcacgaac tggcgcaact
     4921 gctgggtttt gaaaactacg cctttaaatc ccttgccact aaaatggcag aaaatccgca
     4981 gcaggtgctt gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag gtgaaaaaga
     5041 gctggcgcaa ctgcgtgcct tcgctaaagc cgaatttggc gtggatgagt tgcagccgtg
     5101 ggatatcgcg tactacagcg aaaaacaaaa acagcacctc tacagcatca gcgacgagca
     5161 gctgcgtccg tacttcccgg aaaacaaagc ggttaacggc ctgtttgaag tggtgaaacg
     5221 tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc cggatgtacg
     5281 tttcttcgaa ctgtatgacg agaacaacga actgcgcggc agcttctacc tcgacctgta
     5341 tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgcgtaggcc agatgcgtaa
     5401 agccgacggt tcgctgcaaa aaccggtcgc gtatctgacc tgtaacttca accgcccggt
     5461 gaatggtaaa ccggcgctgt ttacccatga cgaagtgatc accctgttcc acgagttcgg
     5521 tcatggtctg caccatatgc tgacccgcat cgaaaccgcc ggtgtatccg ggatcagtgg
     5581 ggtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt gctgggagcc
     5641 ggaggcgctg gcgtttatct ccggtcacta tgaaaccggc gaaccgttgc cgaaggagtt
     5701 gctggataaa atgctggcgg cgaagaacta ccaggcggcg ctgtttattc tgcgccagct
     5761 ggagttcggc ctgttcgatt tccgcctcca tgccgagttc cgcccggatc agggagcgaa
     5821 aatcctcgaa actctggcag aaatcaagaa actggttgcc gtagtaccgt ctccatcctg
     5881 gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgccg caggttatta
     5941 cagttacctg tgggccgacg tgctggcggc agatgccttc tcgcggtttg aggaagaggg
     6001 cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac attctgagcc gtggcggttc
     6061 agaagagccg atggatctgt tcaaacgctt ccgtggtcgt gaaccgcaac tggatgcgat
     6121 gctggagcat tacggcatta agggctgatc attcagtgaa aatctgctta attgatgaaa
     6181 caggcaccgg agacggtgcc ttatctgttc tggcggcccg ctgggggctg gagcacgatg
     6241 aagacaacct gatggcgctg gtgttaacgc cggaacatct ggaattgcgc aagcgtgatg
     6301 agccaaaact tggcggcatc tttgttgatt ttgttggcgg agcgatggcg caccgacgca
     6361 aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt
     6421 tgccggatgt ggtggacgct accgcagggc tggggcgcga tgcctttgtg ctggcttcgg
     6481 tcggctgccg cgtgcggatg ctggagcgta atccagtcgt tgccgcgcta ctcgacgacg
     6541 gcctggcgcg tggatatgcg gatgcggaaa tcggcggctg gttgcaggag cgtttgcagt
     6601 taattcacgc ctccagcctg acggcgctga ccgatattac tccgcgcccg caggtggtct
     6661 atctcgaccc gatgttcccg cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgtg
     6721 tctttcagtc gctggtgggg ccggatctcg acgcggatgg gttgctggag cctgcgcggc
     6781 tgctggcgac taaacgcgtg gtggtaaagc gtccggacta cgcgccaccg ctggcgaatg
     6841 tcgccacgcc aaacgcggta gtcactaaag ggcatcgctt tgatatttat gccggaacgc
     6901 cggtgtaaaa gtaaagcagg gtagcgataa cgctaccctg ctttctgcaa gaataattaa
     6961 tggctttccg gcgtcgcaat catgcgtttc agccacggca ccatcaacag catcactact
     7021 gcaacgccca gcgtgaccag accaatctta ccaaacacgt tggtatagac gggcaacgtc
     7081 tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg ttgccacata gccgcccagc
     7141 aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat caaatgctgc
     7201 ggcaccagag cggcaaccat cgccagaccc agggcgctaa taaacaattc acccaggctc
     7261 tggaacaagt aaaccagcac gataaaccac ggcgatgtca gtccttgtgc atcagcaaac
     7321 cacattcccg cagcggcggc ggtaagaaag cccagcgagc acataaacat gccgagagta
     7381 aatttcatcg gcatagagag atctttgcct ttgctgccca gatgcgtgta aatgcctgcc
     7441 agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct gaccgggttg
     7501 atggaaaagc cgagaatttc atgatgcacg ttgttgatgg caaagaagtt cagcgacgtt
     7561 ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac aaaggcgaca
     7621 aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa gatgatggtg
     7681 acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg catcagccat
     7741 gcacagacga agatcatcac cacgctgcca agtaacacgt acaacagttt gctgaagctc
     7801 atcggccgga agtcgggttc agaaccaatg tctttcacca ttccacgaca ggcgatgtaa
     7861 accagtaatg cgataattaa ccccgccccg cacaggttgt aggtgacaga ataaccgaat
     7921 ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatgtt gatcgacata
     7981 tagaacaggg tgaatgcgcc atcaaggcgc ggatctttcg gcggatagca cttcgaaagc
     8041 aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc cagggcgatg
     8101 aaaatcaggt caggcttaag tagcgacagg ccggtcatga agtagccaat cgccagcaca
     8161 agtgctccga gaacaatggt gcgtttggtc cccagcaggt ggtcgccgac atagccgcca
     8221 atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa agcctgctct
     8281 tgcgagaatc caagctgttt aacgaagaaa accgccagta cgccctgcac gccgtagtag
     8341 ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc
     8401 atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc ctgaaaagta
     8461 tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc ttaatattga
     8521 tgcggtgaat agattaatac actattacaa cagaaaataa ccagctgatc acactaatga
     8581 tggaaaatat gatggtgtta aatattgtat tggctgggga ttttgattgc gaacgcagac
     8641 cgtaggccag ataaggcgtt tacgctgatc aggtaacaag gcgcgccaaa aagcaaaacg
     8701 cccgcatatg cgggcgttca ggaacacgtt gtggagggaa gattattctt cttcgtcgcg
     8761 cagcggaaca atcagcatat caacgtgaac ggtgttgatc agctgacgtg cggaagacat
     8821 cagtttgctc cagaagtcct ggtggtgacc acaaaccacc agatccatat cgtatttctt
     8881 gattgcatcg accagaacct ggcccaggtc gccgctgccg ctcagggttt cagtgattgg
     8941 gtatcctgca ttagtggaaa gctcggtcag cgcatgatgt gtctcttcag agatgcgttt
     9001 ctgcatatca cccagattca catcaataag cccggtgtat aggtcagagt agtttacatc
     9061 tacgtggatc agagaaactt tcgcattgta tgggcgagcc atagagactg ctttctctac
     9121 cagaactttg ctttccgggg agagatcgac cgcgatgaga atgtgtttat aagccatagt
     9181 gttactcctt ccataaagtt gtcgatgact ggccagctag cgtttcttgt gccgttacgt
     9241 cacccgcgtc ctgcgaacta cgtgcgcggg gctaacccag ccgtaaagat tcagtgttaa
     9301 gaccatcctt accttatagc gacccggatg atccgtcaat ccgccttgct tacccgttag
     9361 ataaacaaat tttcgacatg aatatattga tagtggttaa ccttctggaa aaaaaacaac
     9421 ctgatctcct acactatcta tagagccgct cggatgttgg tcaccacaaa agcggtttct
     9481 ccaggacctg gtgttaactg attggcagcg tatcgggagc ggtagccccg gggtggaact
     9541 ccgcgggcag gtcgccgggg aggaggtatg ataagcaccg tcgcattatt ttgggcttta
     9601 tgtgtcgttt gcattgttaa catggcgcgc tatttctcat cactacgcgc gttgttagtg
     9661 gtactgcgta actgcgatcc attgctctat caatatgttg atggaggggg cttttttacc
     9721 tcacatggcc aacccaacaa acaggtgcgt ctcgtttggt atatctatgc ccaacgttat
     9781 cgcgatcatc acgatgatga gtttattcgc cgctgtgagc gggtgcgtcg gcagtttatt
     9841 ctgaccagcg cattgtgtgg tctggtggtg gtcagcctga ttgcattgat gatttggcat
     9901 taagcacttc agcaataata acgcataaaa aaagcgggcc agtaaactga cccgctcgtt
     9961 ttggtgtgta cgattacagg aactgcaagg agagccagta cagcccgccg gaaagcagta
    10021 ccgcagccgg aagggtaaac acccaggcca tcagaatact ggttacggtt ttacgctgta
    10081 agccgccgcc atctaccacc atcgtccccg cgacagaaga ggagagtacg tgagtggtgg
    10141 aaaccggcat cccggtataa ctcgccaggc cgatagacac tgccgccgtc atctgggcag
    10201 acatcccctg agcgtaggtc atgcctttct taccgatttt ctcaccgata gtcgttgcca
    10261 cacgacgcca gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc
    10321 acaccggcgc atactcgatg gtgctaagca tgtcggactt cagtttcttc aacaggcgct
    10381 gatcgtcagc actcacacca ggcatcttca ccactttgtc gatagtgtca gaaacgcaca
    10441 gcataatgcg gcgcatctgg ctacgttgat caagcgacag cttgtcgtag ctttccacat
    10501 cggtagtcaa catgcctttc aggcggttga gcgcgttaat ggtattcgac ggatggcaat
    10561 gaaactccgc aggttgcgtt gcgccagctt ccggagccgg tactaactga tcagcaccgg
    10621 tagcttgttt cagcagcgca ggatgctgct caaagtaagc ttcgacgttg ttgatggcat
    10681 cacgggtacg ggtgatttcg tagccagtgg cattcatgtt caccacgaag cctgctggtg
    10741 cgacaccaat caataccaac ataaccagac caatgccttt ctgaccatcg tttgcgccgt
    10801 gcgaaaacgc cacgccgata gcggaaagga tcagcgcgat acgcgtccag aacggcggct
    10861 ttttcttgcc gtctttcttt tcacgctccg ctggggtcag gtggatacgg gcgcgtttct
    10921 tggtgccgct ccagtagcga cgcagcaaga aaatcagacc gccagcaaac accaggccga
    10981 caatagggga aacgatcaga gaaccgaaaa tacttaatac tttcgggata ttgagtgcat
    11041 ccaccactga cgtcccggtc atcaacgcat tggttaaacc aatcccgatg atcgcgccaa
    11101 tcagcgtatg agagctggat gcaggtaaac caaagtacca ggtacccagg ttccagataa
    11161 tcgccgccag caacatagag aacaccatgg caaggccatg agacgatccc atattaagca
    11221 gcagatccgt cggcagcata tgcacaatag cataggcaac actcagacca cccagcaaaa
    11281 cacccaaaaa gttgaacacc gctgccataa ccacggcgag ctgagaacgc atcgcgcggg
    11341 tatagataac ggttgccacg gcgttggctg tgtcatggaa accattgatg gcttcgtaga
    11401 acagcacaaa agccagtgca agcaataata acagcccggt atgcaaatcc aggccagcaa
    11461 acaaatgtag cataggacgt tacgccattt tgaggacatg aacgcggcgc attatcagtg
    11521 actttcgcgg cgcgggcaaa gtgaaatata gacttttttt gatttgcctc ctgtatggat
    11581 ttcactcaaa aaataattat cttatataat tcaggcaaat acttcctttt agtaatattg
    11641 atgctggtgc gaccactgag gaatctttac aattcacgcc cgttttttct aagaggagcg
    11701 caacgtggaa aggtttgatg ccattattat aggcgctggt gcggcgggta tgttctgttc
    11761 tgcgctggca ggtcaggcag gacgccgggt tctgctgatc gataatggta aaaaaccagg
    11821 gcgcaaaatc cttatgtctg gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc
    11881 gggcgcttat ctgagccaga atccgcattt ttgtaagtct gcactcgcgc gttttaccca
    11941 gtgggatttc attgatctgg tcaataaaca cggcatcgcc tggcacgaga aaacgttagg
    12001 gcaactcttc tgcgatgact ccgctcagca gattgtcgac atgctggtgg atgagtgcga
    12061 gaagggcaat gtgaccttca gattgcgtag cgaagtgctg agcgtggaga aggatgatac
    12121 aggcttcacg cttgaactga acggcatgac tgtcggttgc gaaaagctgg tcatcgcgac
    12181 cggcgggctg tcaatgccgg gcctgggcgc gtcgccgttt ggttataaga ttgccgaaca
    12241 atttggcctc aacgtgctgc cgacccgcgc gggtctggtg ccattcactc tgcataaacc
    12301 gttgctcgaa gagttacagg tgctggcagg cgtggcggtg ccttccgtga ttaccgctga
    12361 aaacggcacc gttttccgag agaacttact cttcacccac cgcggcttat ctggaccggc
    12421 ggtgttgcag atttccagct actggcaacc gggtgaattt gtcagtatca atctgctacc
    12481 ggatgtggac ctcgaaacct tcctgaatga gcagcgtaac gcacatccga atcagagcct
    12541 gaaaaacaca ctggcggttc atctaccgaa gcggttggtt gaacgcttac agcaactcgg
    12601 gcaaatcccg gatgtctcgc taaaacagct caacgtgcgt gaccaacagg cactgattag
    12661 cacattgacc gactggcgcg tacaacccaa cggcactgaa ggctatcgca ctgccgaagt
    12721 gacgctcggc ggcgtggaca ctaacgaact ctcttcacgg acgatggaag cgcgcaaagt
    12781 gcctgggctg tacttcatcg gcgaagtgat ggacgtcacc ggctggctgg ggggctataa
    12841 cttccagtgg gcgtggtcga gtgcgtgggc ttgtgcgcag gatttgattg cagcgaagtc
    12901 gtcctgagct ttttcctaaa gatgaaaatt attagagtcg cattaaaaat gagctaattt
    12961 tgatagtggc tatcttgtga ttattttcta atgagcccgt gaactgaacc ccccatgctt
    13021 aatataaggt ggagggaaag gtgattgaaa actcacccag tgggctgatc tttataaaat
    13081 atgaacaggt tgaaaaaaaa cagtaacttt gctgtttttt atacttaacc actatgcatt
    13141 aatgctgcgt tatttcatga t
//
LOCUS       DAZGCQ010000133        13146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000133 DAZGCQ010000000
VERSION     DAZGCQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.133"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..352)
                     /locus_tag="ACHK07_004321"
                     /pseudo
     CDS             complement(113..352)
                     /locus_tag="ACHK07_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(892..1254)
                     /locus_tag="ACHK07_004322"
     CDS             complement(892..1254)
                     /locus_tag="ACHK07_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104579.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(1245..1781)
                     /gene="yfdR"
                     /locus_tag="ACHK07_004323"
     CDS             complement(1245..1781)
                     /gene="yfdR"
                     /locus_tag="ACHK07_004323"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8104580.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YNVAQHAVLCSKLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            complement(1909..2733)
                     /gene="yfdQ"
                     /locus_tag="ACHK07_004324"
     CDS             complement(1909..2733)
                     /gene="yfdQ"
                     /locus_tag="ACHK07_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO8104581.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(2799..3161)
                     /gene="yfdP"
                     /locus_tag="ACHK07_004325"
     CDS             complement(2799..3161)
                     /gene="yfdP"
                     /locus_tag="ACHK07_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO8104582.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(3830..4504)
                     /gene="ymfK"
                     /locus_tag="ACHK07_004326"
     CDS             complement(3830..4504)
                     /gene="ymfK"
                     /locus_tag="ACHK07_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8104583.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            4595..4795
                     /gene="ymfT"
                     /locus_tag="ACHK07_004327"
     CDS             4595..4795
                     /gene="ymfT"
                     /locus_tag="ACHK07_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO8104584.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            4839..5390
                     /gene="ymfL"
                     /locus_tag="ACHK07_004328"
     CDS             4839..5390
                     /gene="ymfL"
                     /locus_tag="ACHK07_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO8104585.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            5536..5715
                     /locus_tag="ACHK07_004329"
     CDS             5536..5715
                     /locus_tag="ACHK07_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8104586.1"
                     /translation="MRELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            5678..6223
                     /locus_tag="ACHK07_004330"
     CDS             5678..6223
                     /locus_tag="ACHK07_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO8104587.1"
                     /translation="MILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDA
                     PASFLSSAFAHIKVMVGWAGASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEA
                     AIMATVPALTRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVETLINLQAERIRL
                     ADKANFHIHRLLNISGGHRHA"
     gene            6216..6452
                     /locus_tag="ACHK07_004331"
     CDS             6216..6452
                     /locus_tag="ACHK07_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104588.1"
                     /translation="MLNPLILNIYRLFQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFRSGKNMKHLGGAA"
     gene            6449..7267
                     /locus_tag="ACHK07_004332"
     CDS             6449..7267
                     /locus_tag="ACHK07_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8104589.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRW"
     gene            7264..7758
                     /gene="yfdN"
                     /locus_tag="ACHK07_004333"
     CDS             7264..7758
                     /gene="yfdN"
                     /locus_tag="ACHK07_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8104590.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            7758..8411
                     /locus_tag="ACHK07_004334"
     CDS             7758..8411
                     /locus_tag="ACHK07_004334"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO8104591.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGTAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8408..8734
                     /locus_tag="ACHK07_004335"
     CDS             8408..8734
                     /locus_tag="ACHK07_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO8104592.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKA"
     gene            8731..9120
                     /locus_tag="ACHK07_004336"
     CDS             8731..9120
                     /locus_tag="ACHK07_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8104593.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            9140..9949
                     /locus_tag="ACHK07_004337"
     CDS             9140..9949
                     /locus_tag="ACHK07_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO8104594.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVQELTDTQNLGVGPVNTVRGGPKQGSYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVVSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLRQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            9957..10946
                     /locus_tag="ACHK07_004338"
     CDS             9957..10946
                     /locus_tag="ACHK07_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8104595.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            10960..11712
                     /gene="ydfT"
                     /locus_tag="ACHK07_004339"
     CDS             10960..11712
                     /gene="ydfT"
                     /locus_tag="ACHK07_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO8104596.1"
                     /translation="MNLEALPKYYSPKSPKLSDAAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKAGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEPCAGTGFHNVLREVVKYSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(11984..12073)
                     /locus_tag="ACHK07_004340"
     CDS             complement(11984..12073)
                     /locus_tag="ACHK07_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104597.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLTVIALLIM"
     gene            complement(12128..12340)
                     /gene="cspF"
                     /locus_tag="ACHK07_004341"
     CDS             complement(12128..12340)
                     /gene="cspF"
                     /locus_tag="ACHK07_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO8104598.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            12641..12856
                     /gene="cspB"
                     /locus_tag="ACHK07_004342"
     CDS             12641..12856
                     /gene="cspB"
                     /locus_tag="ACHK07_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO8104599.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNFRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
BASE COUNT         3331 a         3026 c         3376 g         3413 t
ORIGIN      
        1 tcaggcctgt atttctgacc agttctcctg tttacgctgt tccagcgaag aactgtcgat
       61 actgaaacac cacagaaatc ggcgacttgt ttagttgtca ttaagttgtt cattacttca
      121 cctcctgcgg cggctccggt agtggcatcc agtgggttac tttcgatgcc tgttcttcct
      181 cattgtcagt aactgcccac catttgtttc tcgaccaatc gtaataccct tcgaaggtat
      241 cgcactcagt ccagccgtaa gacttccccc aacaccaaac atactgttta tcattcggca
      301 ttcgctcact acagcttatc caaccacccg gacttaccgg agagttgcca gacagcgcgt
      361 tctgcagccg ttccagctta acgtattcct gaaccctgtt tccgtcgcac acctgaagcc
      421 attgcgcagc cttttgcgca tcagtgtgaa aggcacaagt gcgaccgtca tcaaattgca
      481 tttcgtagag gtcagcaact tgttcaaact gcgtgtgtgg tgacttgtaa gtttggttta
      541 caggttcggc acaatgaagc atggcggcgc ggcaggcgtt ccagccttca tcaaaaccga
      601 ctatgccatt atttaaagac ggacgagcat ctggcactac cggcactggc ttggctatat
      661 atagcggctg aacataccag ccctttgata accaactgtc agcaatgttt ttactcctcg
      721 ttattgccgg aatacctaag ccattttctg aatgcagcca tgccaccggc tcctcttcta
      781 gcgatgccag agcaatttca taagcacggc gctcaatatc gtctcgaacc tctaggctgc
      841 tgattcgttc tttgatttct ttaatcagtt ctttattggt aaatgtggtc attatgctcc
      901 agcctccggt gcttttggca ttactgccca gtgagtgata ttgacgtttt caaggtcccc
      961 gacctgaaat gtccactgcc attctccggt ttctttttgt ccccaggtgt accagagaga
     1021 acgccagcca atcagccagc cttctccatt agcatcaaat aacagaacac tttcatttgc
     1081 tggtggcagt tcagctgaca ctggtattat tttgttttcc agtgccgcac atttagcttc
     1141 aagcgcgtcg aatttacgta ccaggtactc agcatttgtt tcgttcactt tcagatctcg
     1201 cggtacacat ttcccgcgaa gaaacccttc catttcgaaa acattcatgc gcatttgcgt
     1261 aactccgata aatcgttaaa acgttccata aacatcccgt aggcatggcc cggtgccagt
     1321 ggaatcacgt tgaacatctc tgttgccggg atgccttcca gtacaggcca gaaagagcca
     1381 tcatcaagcc cgagatcgcg gcgttcggtt gccagcatga tgagatcggc atatttcacg
     1441 ggtgtactca taaccggggg taacccgtat ttctcacgga ttacggcgtc tattttttct
     1501 tccatccgtt tatagtcagg aagaaggcgt ttcagtggtg cgggaatgtc ctggcaatac
     1561 gcttctgttg catcatgcat taacgcttca aaagcaaatt cctgcggcac cagtttgctg
     1621 caaagcaccg catgttgggc gacgttgtag aagtgcgaaa gatgaccggc aaagcgacag
     1681 atatttgaaa gggaaaccgc gatatcgtta atatcgatgt cgtctttatt tatcctgtca
     1741 taataaaaat gcttcccaga aaaagttttg ataaatgaca ttttgttctc cacgtatatg
     1801 cgctgcaccg cgctgaattc tggtaaaaag aatccttcac catccggcga ttattgagta
     1861 aattacgttt ccataaatgc ccccgcaggg gcatttgcag taatgaaatc aggcggtgaa
     1921 agtaccaata aaggtttcta ctttgctgtc cttgaatttc tcaacaagca gatcacgaaa
     1981 ttcgttagcc atttcctcct gcaccgcctc cagctgaata atgcgcagaa ccagtacagg
     2041 acgatcgcca gtgataatgc tgaggcgtaa tttaaacgga cgttctttca gaccttcaaa
     2101 cggaacgcat ttaaattcaa atgccactgg cataatgtct ttggtcttcg cttcgacaga
     2161 ctccatcagg gagcgtttgc cgctgaagtc attatcttca aaatcagcgg tctggtttgc
     2221 ttcaatcgtg attttacgga ccgccgcagc cgcttttgtt gcctgaatgg cgtcaccatt
     2281 agcatcaaag cccacaaggt agtcggccca gtcttcgatc cattctgcca gtgacttctg
     2341 ggagttacgc tcgccgttaa cagacaacag agcagagaac tgtgctgtct ttttcagttt
     2401 gagagtggca gtgttatctg cgtgacctgg ctcatcaatg gtacccaggt taagcacact
     2461 gacggcacgc atattatcgg catcgataaa gcagcgggtg ccttcatctg caagatcttt
     2521 agaataacgg gtaaagtcat cgatgctggc agtggaaagc gcaccacgga aacggaagcg
     2581 atttaaatta aatttttcca gatcatgaat gcggaaattc tcaggcaatg ccacagcatc
     2641 ggcaccaatc ttactgataa tttcattaac accctgagca gaaataaggg catggatttg
     2701 attaattgcg gttgcgtcta agttctgaga cataataagt cctcactata taaagatatt
     2761 cagtgatgag ataaataatc agttaattaa gaacgatatt aatgacctgc tgcgcgtagt
     2821 tttccgtcag gttcaccggc aagagtcagt aattgtccct ggtcttcctg cagaatagtc
     2881 aggcgaccac cgcgattgac atacatcggc gtttcggtgg tgtcttcttc ggaaattttc
     2941 ccgcggttag tcgggcgaac atatgagagt ttgtgtttga ttttcacacg gttctcatca
     3001 aatggttcga tttccaggtt gagtgagacc ttacctttgg ttttcgtgtt catcacaccg
     3061 gaagcgactt cactgagaac tgcgccgatt ttggtttcaa atacgccgcc gtccagctcc
     3121 ccgataaatg cctgcacatc agtactgcgt tcgctagcca ttttgctgct cctcatcata
     3181 tcgaccctgc aaggccggtt ggtttctcca caaaacagag aagaacacct gcggtggcag
     3241 ccgcccggat ggattgggtt atgagcccgt cgtccggtga tgctcttctc tgttttgtaa
     3301 aaagagcggt accagccgga agcaagtgta caaactggta ccgccaaagc agtggctgtt
     3361 gtggtggggg tgtcactcag gcgtatggtc aacctgacaa tccggtgtcc tcaacgggga
     3421 aagagtaacc cagccatact taccgccgcg ccatttcgcg gatgaccaca acgctgagag
     3481 cacttagcca gttacggcac cacactttgt cgcggctcca taaatgccct catcgttgca
     3541 ccctggtctc ttcccaggcg tcaaaccgga tcgccacgct ggttaggcgt cttatcagca
     3601 tcatcattga cttgcacatt ccggctacct ggtttgtttg cccgagcaag gagtggattg
     3661 tcccctttaa cgtccccaga ccgctaacga cgcatgtgcc atacgccgtg ttacaaccaa
     3721 attttgtttg aatcttgcct gcctcatgtt tcttttggat acattatgta tctcatgggt
     3781 acattgtcaa gtataaaaaa acctgccgaa gcaggttcat aaatattgat taggccttta
     3841 ttgtgtatct tcttggtttt cccgagaaaa tcactgtacc aattatagag caattaccgt
     3901 taatcttaat gtaaggctca ggccagtttg ggtttaatgc tttgagataa cgctgtgttc
     3961 catcttctat caatcgcttg aaggtggttt cgcctgtatc gtgcatcaat gcaataacgt
     4021 cgtcaccgtg gcaggcaggg acttcgggat caacaaaaat catgtctcca gggcggtact
     4081 catcaatcat tgaatcacca atcacccgca agatataagt catttcgcca cagggtacag
     4141 ggcagggata cgtttctgct gtgctcaaat caacctcaga atagccaact tctttccatg
     4201 ctccggcctg tacccatgat atgacaggga ctaacgttat ttgtttgtta gtgattgaaa
     4261 catcaggttt ttttgtgatg ttcgttgtct ggtgttcttg atcaagccat ccgacaggca
     4321 ggtcgaaaca tttttcgatg tgccgtgcca tgctgtcacc gatattttta gtagcgccat
     4381 cccccataaa cctgctggtc tgggttggct cgcgatcaat catggtggca aaggatgaat
     4441 ttccgccaac accatctctc agttttctgg cgttagaccg ccggatgtca tggattgttt
     4501 tcataacgaa attaaaacct ttgtaccgat aaggtacaag tatcttgaag gttcatttca
     4561 atcatgtaat atgtatatcg gaggtacata ttgtatgaaa gcgtattggg actctttaac
     4621 caaagaacag cagggcgagt tggccggaaa agttggctca acacctggct acttacggct
     4681 ggttttcaat ggttataaaa aagccagttt tgtgctggcg aaaaaacttg agcaatgcac
     4741 gtcaggtgca attacgaaat ctgacttaag accggatatc tatccgaaag attaacagaa
     4801 caccttcaat ttttaaccac agaacgatga ggctaaccgt gggtaagtat cactggaaag
     4861 tagaaaaaca gcctgagtgg tacgtgaaag ctgtcagaaa aactatcgca gcgttgccgg
     4921 gtggttacgc tgaagcagct gactggctgg atgtaacaga gaacgcatta tttaaccgcc
     4981 ttcgtgccga tggcgatcag attttcccgc tgggatgggc aatggtttta cagcgcgcgg
     5041 ctggcactca ctacattgcg gatgctgtcg cacagtctgc tggtggggtg ttcgtatcgc
     5101 ttcctgaaat tgaggaagta gagaacgccg atataaacca gcgcctgctg gaagtcatcg
     5161 aacagatcgg gaattactca aagcagattc gttcggcaat cgaagatggg gtcgtggagc
     5221 cacacgagca gacagcaatt aatgatgagt tgtatctgtc aatttcgaag ctccaagagc
     5281 atgcagcact ggtctacaaa atcttttgcg ctccagaaaa gagtgacgcc cgcgagtgtg
     5341 cagctccggg cgtcgtggcg ttttgtgtct gtggagaaac taacgcatga acagtttaac
     5401 ggcaaataac cgtttgtcgc aacagctggt ggtcagtgtc gctgaacacc tgttgttacg
     5461 gcatgaatgc agattaccaa atcacctggc tgtaagtaac cacagagaac tttacctgac
     5521 tgtggggggc gagttgtgcg ggaacttaac cgctggtttc gtgacggaag agggctttat
     5581 gtccatgtta ttcgttggga accagaaaca cagcgcgtta tctatcttcg caaagactac
     5641 ccgcatgagt gctttagtcc tttgtggaaa ttcaggcgtg attttgttga gtgtgaagga
     5701 ccaccagcac attgattctg ccattccggg acgttacact gttcaggcac cttataaagc
     5761 gggtgccggg attggcgtcc tggaattgat caaggcgata tatgacgcgc cagcgtcttt
     5821 tttatcgtcc gcatttgctc acatcaaagt tatggtgggc tgggcggggg catcgaaaga
     5881 tgcgccggtt tccttgatca ccggttacgc caaccccgtt cagttcacca ccagcgaaat
     5941 tggcgtttcc ggtggtggaa gtatttcacc gatcaaggag gctgccatca tggctactgt
     6001 cccagccctc actcgtctga atgatgaaga cttacataaa ctcagttatg taacaactgc
     6061 actacgtgct ctgcgcaagg taactctttc ggatccgcag gcgcatcagg ttctggtaga
     6121 aacccttatt aacttgcagg ctgaacgtat tcgtctggcg gataaggcta attttcatat
     6181 tcaccgtctc ctgaatatca gcggagggca tcgtcatgct taatccgttg atcctcaaca
     6241 tttaccgttt atttcagcgt aaaaaaacat caattcctac agttgggcag tggtacacca
     6301 cgcctgcagg gcatgttcta cgtgttagcc tggttgaccg tgaatgtcag aaggtgattt
     6361 gtgaaccgct gggccgtaat taccgcgtca gtatgccgct tataaccttt cgctccggaa
     6421 aaaacatgaa gcatctcgga ggtgcagcat gagtatggag ctgatggtta aagcgatgaa
     6481 aattcgagtg ggtaatccat tgcgaaaact ggttctgatc aagctggctg ataatgccag
     6541 cgatcagggt gagtgctggc ccagctacca gcatattgct gaccagtgcg agattagcaa
     6601 acgttctgtg atgaatcata ttgcggccct ttgtgagtcc gggctggtaa aaaaagtcac
     6661 ccggaaaggt gaaaaaggta actcaagtaa tatctatctc cttcatctgg atggtgcagg
     6721 agattcacta gggggtagtg caaataattc actatctggt gcagcaaatt caccaggtag
     6781 tgcaggagtt gcaccagggg gtagtgcagg agattcaccc agaaccagtc actcttttga
     6841 accagtcaaa gaaccagtca atgaaccaat agctgttggt gcatcagttg atgagtccgt
     6901 gcgagttcgt tcaaaccgac cggaatattc tccggagttt gagcaggcat ggctggcata
     6961 tcccaaacga gctggtggca attcaaaatc tgcagccttc aaagcctgga aagcccgttt
     7021 gaatgagggg gtaaaccccg aaaccatgct ggaaggtgtg aaacgctatg cgggctgggt
     7081 atctgcgatg ggtaacagcg gcacacaatt tgtgaaacag gctgtcacgt tctttggtcc
     7141 ggatcgtcat ttcgaagaat cctgggaagt tcctgcggta tctgcagcca gacgtgagga
     7201 cccgtacttc aaatccagtt acgacaacgt ggactacagc cagatcccgg caggattcag
     7261 gtggtgatca tgagtctttt gaatgacgtt cagaaattca ttgaagccca tccggggtgt
     7321 acttccggag acattgcgga tgcttttgca ggttactcac ggcagcgcgt actgcagtca
     7381 gcaagcaagt tacgtcagag tgggcgtgtg gctcaccgtt gtgaagggga tacacgcaga
     7441 catttcccgc gcctgactga gagagcgcag gagccggaac cacaaccagt tcgtgaaacc
     7501 agacctgtgc gcaatttcta tgtcggcact aacgatcccc gggtgatttt gtgcctgacc
     7561 cgccaggctg aagaactgga gtcaagaggc ttataccgtc gtgctgcaac cgtgtggatg
     7621 gaggcattcc gtgaaagcca ctcccagcca gaacgaaaca attttctggc gcgtcgtgaa
     7681 cggtgtttac ggaaaagcag caagcgggct gcatcaggtg aagagtggta tctgtcaggg
     7741 aattacgtgg gggcttaatg agtaataaat attgccaggc gctggtggaa ctgcggaaca
     7801 aaccagccca tgaactgaag gaagtgggcg atcagtggcg cacgccggac aacattttct
     7861 ggggaattaa caccctgttt ggcccgtttg ttctggatct gttcaccgac ggtgataacg
     7921 ccaaatgtgc cgcgtattac acggcggaag acaacgcgct ggcgcatgac tggtcagaac
     7981 gtcttgcgga gcttaaaggt gctgcctttg gtaatccccc atacagccgc gccagtcagc
     8041 atgaggggca atacatcacc ggcatgcgtt acatcatgaa acatgccagt gccatgcgtg
     8101 ataaaggcgg gcgctatgtt ttcctgatca aagctgccac cagcgaagtg tggtggccgg
     8161 aagatgcaga ccatattgct tttattcgcg ggcgtattgg ttttgaactg cctgcctggt
     8221 ttatcccgaa agacgagaag caggtgccga caggcgcttt cttcgctggt gctattgctg
     8281 ttttcgataa gacctggaag ggaacggcaa tcagctacat cgggcgcgat gaacttgagg
     8341 catgtggtga ggcgtttctg gcgcaggttc gccagcaggc ggaaaaactg gtcagggaga
     8401 tggcggcatg acgacattaa ctcaatgcca gcagcaggtg ctggatatgc tgatttctta
     8461 ccagaaagaa cgtggcttcc cgccaaccaa tcaggaggtg gcaaccatgc tgggataccg
     8521 ttcagtgaat gcagcggtgg agcatcttcg cgcactggag aaaaaaggcg tcatcacgat
     8581 aaagcgtggc gtggcccggg ggataacgct tcataccgcg gtgaaggacg acgacagcga
     8641 ggcggtcggg attatccgct cactgcttgc cggtgaggaa aacgcaaggc tgcgtgcagc
     8701 ccactggtta catgagaggg gcctgaaagc atgaagctga tcctgccttt cccgcccagc
     8761 gtgaacactt actggcgaca ccctaacaaa ggggcgtttg caggtaagag cctgataagc
     8821 gcggcggggc gaaaattcca gagcgcggcg tgcgcagcaa tagttgagca gttacgtcgt
     8881 ctgccgaaac caacgtcggc acctgcttca gtggagatcg tgttgtttcc tccggataac
     8941 aggatccgcg atctggataa ctataacaag gcgctgtttg acgccctgac ccacgcaggt
     9001 gtgtgggaag acgacagcca ggtgaaaaga atgctggtgg agtggggacc ggttatcccg
     9061 gaagggaagg tcgagatcac tatcagtaag tacgagaaaa cggcgggtgc agccgcctga
     9121 ttaagaggag aaacgaagta tgaataatct gatggtcatt gatggtattg aagttcgtcg
     9181 tgatgcttat ggacgttaca gcctgaacga tctgcatcgt gccgcagtag catctggtgc
     9241 aaatgccaga acgaaggagc cgggaaagtt tctttccagc caacagactg ttgaacttgt
     9301 tcaggaattg accgataccc aaaatttggg tgtcggtccg gttaatacag ttcgtggtgg
     9361 tcctaagcag gggagttatg tctgcaagga actggtgtat gcctatgcaa tgtggatcag
     9421 tccgtcattc catctgaagg tgatccgtac tttcgatatg gtagtcagtg caccggaaaa
     9481 attatccggg caggctgctg acaagatgca ggcaggtgtg attctgctgg actttatgcg
     9541 tcgggagtta aacctgtcta actcatctgt gcttggggcc tgtcagaaac tccaggaggc
     9601 tgttggctta ccgaatctgg caccgcgcta tgccattgat gctcctgctg atgcacacga
     9661 tggctcaagt cgccccacgc tgtcgctgag tgcactgctg aggcagtatg gtatccgcct
     9721 gacggctaat caggcatatc accagatggt gaagctgggg atcgttgaac aacgcgaacg
     9781 atacagccgt accgcgatta acaacatcaa aaaattctgg tcgctgacgg cgaaaggctg
     9841 catgttcggc aagaacatca ccagtcctgc aaatccgcgc gagacgcagc cgcatttctt
     9901 cgaatcccga ttccctgagc tgttaaagct gctcgatacc gttcattgag gtgaccgtga
     9961 gagcactact gacccctgaa attgccccgc gtatggggat cgtattgttc aggccaggtt
    10021 cagagctgat gcccctgttt atgcaggggc gtgtcctgct ggagcctgag ccggaacgtt
    10081 attcatcttt cgccagtggt gccgttccgg cggcatcaca accgctggcg gatgatcctg
    10141 ccgttcgggc cgtgttccgc aatgaggcag tgatccgtcg tgctggtggc gtggaatgtc
    10201 ttgaaagctg gttacttcgt gaaaagggct gtcagtggcc tcattccgac tggcacagcg
    10261 agaacatgac cacaatgcgg cacgctccgg gagcaattcg tctgtgctgg cactgcgata
    10321 accagctgcg tgaccagttc acggaacggc tggaatcaat ggcaacggat aactgtgccc
    10381 gctgggtgtt gtctgtcgtg cgtcgggatc tcggttttga tgacagtcac gttgtgacaa
    10441 tgccggaact gtgctggtgg ctggttcgta atgatttggc ggatgcctta ccggaaagtg
    10501 cagcccgtaa ggcactgaga ttaccgaagc ctgttgtgcc atctgtcacc cgggaaagtg
    10561 accttgtgcc ttcggttcct gccaccagca tcatccagga taaagcgaaa aaggtgctgg
    10621 tgctgaaagt ggatccggag tcgccggagt cttttatgtt acgccccaaa cgtcgccgct
    10681 gggttaatga aaagtacacg cgctgggtta agacgcagcc gtgcgcatgt tgtggaaagc
    10741 cagctgatga tccccaccac ctgataggcc acggtcaggg tggaatgggt acaaaagcgc
    10801 atgatctctt tgtgttgcct ttgtgcagaa agcatcacga cgagctgcat gcggataccg
    10861 tgacatttga agagaagtat ggctcccaac tggagctgat atttcgtttt atcgatcgtg
    10921 cgctggcaat tggcgtactg gcgtaagtgg agaacgagca tgaaccttga agccttacca
    10981 aaatattact ccccaaaatc tccaaaattg agtgatgccg caccggcgac aggctcaggt
    11041 ggtttaacga ttacggatgt gatggctgcg caggggatgg tgcagtcgaa agcaccgctt
    11101 gggtttgcct tattcctggc aaaagctggc gttcaggatc ctcagtttgc gattgaaggt
    11161 ctgctcaatt acgcgatggc actggataac ccgacattga acaaattgag tgaagaaatc
    11221 cggttacaga tcatccctta ccttgtgagt tttgcctttg ctgattattc caggtctgcg
    11281 gcaagtaagg ctcgctgtga gccttgtgct ggtactggat ttcataatgt attgcgcgaa
    11341 gtggtgaaat actccagaag cggggagtct gttatcaagg aagagtgggt gaaggaacta
    11401 tgtcagcatt gtcatggtaa gggagaagtc agcacagcgt gcagagggtg taagggtaaa
    11461 ggtattgtcc tggatgaaaa aaggacccgg cttcatggcg cgcctgttta taagatttgt
    11521 gggcgttgca atggaaaccg gtttagtcgt ttaccaacga cactggcgcg gtgtcatgtc
    11581 cagaagctgg taccagacct gactgattat cagtggtaca aaggatatgc agatgtcatt
    11641 gataaactgg ttacaaagtg ctggcaggaa gaagcatatg ctgaggtgca attaagaaaa
    11701 gtgacgagat aaatgatttt cgccgaagat ggcgacgtaa tgcttgcatt tttcaaaaaa
    11761 tatggataaa attctttcaa cgatgggctt tatatacccg acgttaagaa aaagtagaaa
    11821 acccgctgat gagcgggttt ttgtgcttta aatagggtaa tagagatgtt gaatctcatt
    11881 ccgggattca tgtttgttta cttattattt atcgggtgac tttgcctgat gtttaaaatg
    11941 ttttcttcca gtgctatgtc cctagataca atgagtctgc atattacatt attagcagag
    12001 ctattacggt caaagtacag cataagcttt taaagccaat caaccagtca tcaagacaga
    12061 cggggttatt catgaaaact ctccatgttt gattcgatgg ggcctgaaat taaagcttta
    12121 atatagctca tgaaaggtaa acattggcag ctgaagggcc acgcagacca tttatccggc
    12181 aaaattccac gcgtaatccg gtggtaattt cttctgcatc gcggagattg agcgctgaaa
    12241 catgaagctg gacatcgata cgaccatcgg atggggtgat aagacccttg ccgcttttgc
    12301 cgtcaaaggt tttgacaatt cctgtcattt tacgggacaa aaaaattcct taatactgat
    12361 aactgggcgc actatacaca cgttcctgaa gaaagctata gttttttgat ggggttgaag
    12421 atggctggat gtctaaaata aacattgctt catatgttca actatgcgtt aatgattgcg
    12481 tcggtttgaa gaacagacga tatacgaagt agtttactaa agcagttctc atttcaggtg
    12541 ttattcactt attccttctt tgagtctctc caattaagta cgaagtcgtt tctgttatac
    12601 aagccattta tgccgaaagg ctcaagttaa ggaatgtaga atgtcaaata aaatgactgg
    12661 tttagtaaaa tggtttaacg ctgataaagg ttttggcttt atttctcctg ttgatggtag
    12721 taaagatgtg tttgtgcatt tttctgcgat tcagaatgat aattttcgaa ccttatttga
    12781 aggtcaaaag gttaccttct ctgtagagag tggtgctaaa ggtcctgcag cagcaaatgt
    12841 cataattact gattaaaatt catagtttgt ctgtatacga taacgaagaa ggctgatgcc
    12901 tgagtggaga tacagacaga gtggtgaata ttggatctct ttaataaata gtaaggaggt
    12961 ccaatacatg aaacaatggc cagcatattt ggcaataact taatcaggaa aagtatgcta
    13021 accattgtgg tgaagtgcag gtttgctgca tgaatagttt tacagcagaa gctaactgct
    13081 ggcatagcaa aacaaagtgc gtaagtggat gactcccaca aaaagtacca caatattaaa
    13141 cccgct
//
LOCUS       DAZGCQ010000134        12828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000134 DAZGCQ010000000
VERSION     DAZGCQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.134"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..136)
                     /locus_tag="ACHK07_004343"
     CDS             complement(<1..136)
                     /locus_tag="ACHK07_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8104600.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     R"
     gene            complement(133..384)
                     /locus_tag="ACHK07_004344"
     CDS             complement(133..384)
                     /locus_tag="ACHK07_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO8104601.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            869..1735
                     /locus_tag="ACHK07_004345"
     CDS             869..1735
                     /locus_tag="ACHK07_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8104602.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKEKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            1732..2439
                     /locus_tag="ACHK07_004346"
     CDS             1732..2439
                     /locus_tag="ACHK07_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO8104603.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            2441..2866
                     /gene="nudI"
                     /locus_tag="ACHK07_004347"
     CDS             2441..2866
                     /gene="nudI"
                     /locus_tag="ACHK07_004347"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8104604.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(3399..4040)
                     /locus_tag="ACHK07_004348"
     CDS             complement(3399..4040)
                     /locus_tag="ACHK07_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8104605.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYREPTTD"
     gene            complement(4331..4813)
                     /locus_tag="ACHK07_004349"
     CDS             complement(4331..4813)
                     /locus_tag="ACHK07_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104606.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG
                     "
     gene            complement(4930..5778)
                     /locus_tag="ACHK07_004350"
     CDS             complement(4930..5778)
                     /locus_tag="ACHK07_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8104607.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMRGKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            complement(5824..6088)
                     /gene="yacB"
                     /locus_tag="ACHK07_004351"
                     /pseudo
     CDS             complement(5824..6088)
                     /gene="yacB"
                     /locus_tag="ACHK07_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(6088..6357)
                     /gene="yacA"
                     /locus_tag="ACHK07_004352"
     CDS             complement(6088..6357)
                     /gene="yacA"
                     /locus_tag="ACHK07_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO8104608.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            6518..6757
                     /locus_tag="ACHK07_004353"
     CDS             6518..6757
                     /locus_tag="ACHK07_004353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104609.1"
                     /translation="MSVVLLPPARPVLTRDMERVLERSGEAPVYWPNLHASFRLREDA
                     GFVPCVRLICSWPWHGYGQILLRNSIGVPDLLKVP"
     gene            6990..7352
                     /locus_tag="ACHK07_004354"
                     /pseudo
     CDS             6990..7352
                     /locus_tag="ACHK07_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            7356..7711
                     /locus_tag="ACHK07_004355"
                     /pseudo
     CDS             7356..7711
                     /locus_tag="ACHK07_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            7992..9131
                     /locus_tag="ACHK07_004356"
     CDS             7992..9131
                     /locus_tag="ACHK07_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO8104610.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            9251..10519
                     /locus_tag="ACHK07_004357"
     CDS             9251..10519
                     /locus_tag="ACHK07_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO8104611.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            10527..11672
                     /locus_tag="ACHK07_004358"
     CDS             10527..11672
                     /locus_tag="ACHK07_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO8104612.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            12140..12286
                     /locus_tag="ACHK07_004359"
     CDS             12140..12286
                     /locus_tag="ACHK07_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104613.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(12612..>12828)
                     /locus_tag="ACHK07_004360"
     CDS             complement(12612..>12828)
                     /locus_tag="ACHK07_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO8104614.1"
                     /translation="TAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIE
                     HYNETHPHSALGYLSPREYRRQRVTST"
BASE COUNT         3279 a         3138 c         3023 g         3388 t
ORIGIN      
        1 cacgtagttt tgcagacagt atatgaggat ttttgctaca tttttttagt tttttcacaa
       61 actgttgttg aatggtctta tccagtttca gccattcttt aagcgcatca tccctgaatt
      121 ttatcgtata agtcatgaat aactgtccag gtcaacatca tgcagaggtt gactgctacg
      181 ttcagtaacc agtttcacca gctcctggtc atccagagca tcaagcatcc tttcatacag
      241 ttcagcagga acacagtaga atgccggctg attgcgattc agaatggcga ccggataacc
      301 atcaccagca ccgactgtcg ccatcggatt ttttttcagt tcactgacac ttgcactgat
      361 gtcagacaga atgatgttag gcataatgct ccttttaaga ctgtttaata agtcttaaag
      421 atacatctga aaggacccgt taacaagtct gtattactca tacatttgca aataaattta
      481 atgctgattt tttactctga tgccgcacca gcgcatggaa ggtggcacac agtggttaca
      541 tataacacag gagtaaaatc ggcgtaagcc ggtagaagaa ctaaatattc tccagttatc
      601 tcccgtcttg tgaggcatac ggcctgtcag tgatgaaaat gcttctgtgc acaattttca
      661 ctggtgtgac tgatagcgtc cctccgatca tgggcttcca gtaatgcgat taccggaact
      721 catcattcat gagttccttg cgcaacccac tgctctgatt actggcagat attatatcct
      781 aggtaagccg aaggttgcgc tttgtcatgg tctgcaataa ccattatgtt ttaatggtta
      841 ttgatgtatc atagtcagag gacttgttat ggatattagc acccttcttt tagatttcag
      901 aataacttca cattgtaacc taggatgtga tctgtgtttt cgtacacctg gaattcagga
      961 ctcttctctt gctagtgctc gtaatgtaat tgagcggatg tataaaatgg gattccgtcg
     1021 tatcggtttt accggagggg aacctacatc ccggagtgat ttcattgatc tgattgaata
     1081 cgctaagcaa ctgggtttta tgacatatct ctccacggtc gggcatcgat ttattatgga
     1141 tcatgctcgg ttaaatccgg tgttagactg ggtaggaata ccaattgatg gaattgacag
     1201 tcttgtcaat tcagaaattc gcagtgataa aatgagtaac caacacaggg ttatcaaaaa
     1261 tatattaaca tggttacccc aaaataataa tcgaattaat ataaaactca cgaccgtcgt
     1321 atctcagtct aacattaatc atctcagtga tattgtttgc tgggttgatt ctctgcccta
     1381 taagatccaa gcttggcgat tttaccagtt ctgtcctcta ggagtcggaa aagaaaaaag
     1441 agataaactg gaaatcaata cggatatatt taaagagaaa atggtaatgc taaaaaaaca
     1501 acaccctaac atgcccatta gttgggcaac tttcgaagaa cgggacaaag ccaatgttgt
     1561 tatggaaccc aattttgaca ttattatccc tgatggagaa aactatactc tgttgggaaa
     1621 tatgctgaca gattcacaag atcatataat cagtgcaatt ttcggaaata atgaaatcat
     1681 gcaaaaatgt aagaataacc gcttttggtt ggacaaaatg gagtttacag tatgagatat
     1741 aattacttgt atatggatcc ttggtcagca gataaaaaag agaagaataa aactcgaaat
     1801 cctctgtata tatgtctgtc gggtaattcc ggtgtaggta aaagtactct tctgagacgc
     1861 atgtcaaagg agttatttaa ggtagatcca gacactgtcg cgatagatga aaaaagtgtc
     1921 caccacactc tccttcctta cctttttgac gataccagta actacggtta tctaatccag
     1981 cttaatttta tgatacaacg agcattactg ataaaatctt ggctggataa agggtttaac
     2041 cttattatgg agagatccca tattgaggat tatattttta tcaacttcat gctgaagtcc
     2101 gaatatataa ataaagcaca atatgatgca tacatggtgc tctggagtag aataaatgaa
     2161 ttacttacag agccagacat tatagcattc attaattatc ccatcgcaca ttcactcatg
     2221 catctcaatg atgatgaaat tcatggtgta cggcctgaag aatttccaga tgaggaaatg
     2281 aagataaaat ggatcacagg atggcatgcg gaatatcaac attttactga taatcttccg
     2341 ttacaccttc gtgagcggat tatggtatgt gctcacccgg atgacattga tgatttttct
     2401 gataaggtta tccaaaaagc catgatgttg aggacataaa atgcgccaga gagtaatagt
     2461 gtgccctgtg atacaaaatg aaggtgagtt tctactttgt aaaatggcct ctgatcgcgg
     2521 agtattcccg ggccaatggg cactgcccgg tggaggaatg gaacccggag agactatgga
     2581 aacagcattg aggcgtgaaa taaaggaaga actgggaaaa gaactgcata tcactgacgt
     2641 aaaaccctgg gcctttcgtg atgatatccg tacaaaaacg tacccggatg gtacccacga
     2701 ggacatttac atgatttacc taatattcga ctgtatcagt acaaaccgta cagttacatt
     2761 taatgaggag tttcaggata tagtctgggc atctcctgac accctaaaat atatggattt
     2821 aaatgaagca acccggatca cattttctca gaaaggtatc ctttaaactc caatgctaat
     2881 cgtgcaactg ataacggctt ccacaacagg gagccgtttt cttatgccac tactcaagga
     2941 tggggtttga gggccaaccg tacgaaaacg tacgatttcg gacagcgaaa cagctgttgt
     3001 cccgccatcc agacgattcc tgaaagtcat ttgtctaaaa aatagtctta acgtggttta
     3061 ttttccgaat tccaagcggc cccctggatg agcgtgatga ttactggcgg acctttgacg
     3121 ttccagcaaa ttcccctgtt ccacataggt ttccatatat gaccggttat tttcacgttc
     3181 ggtgtacgtg ttacagctgc aaataataca ggtgcattat caatatcatc agggataaaa
     3241 ttgtaagatc gtggaaaatg tttattcata gcccggatga tggctgtctg tgcatccttt
     3301 gttcgggcgc ggtgtttcca ttcacgccag ccatccacct cagaaataat aaggggtcag
     3361 tttggatatc gaaaattatt gtacgttaag gctacttttt aatcagtcgt tggttctcga
     3421 taaagtgcct gaatacggca aacctgcgaa gtgctgtagc ccgtagcatc tgctgtttct
     3481 ctaatgctga gtttttttac ttgtctgtag tacaacactt tctgatgccg ttcctgatcg
     3541 gcttgttttc cccggtattt acccaacgta tgagctcgtt caattccctg tttttggcgt
     3601 tggcgacgac tcagccagtc cttatgcgac atcgcggcca tcaggtctat aagcatatta
     3661 tttattgcag ttatcactgc gcgggtaatc ggatctgatt gtgaaggatc tttatctgaa
     3721 agggcttgcc atgatgttgg aacatccaga ctgacaattc gcaattcatg ttgttcaatt
     3781 tgctttttca gcgttatcca gtcgctgttg ctaagacggg tcaggcggtc tatctgctca
     3841 accagtaaaa tatcattacg gtgactgtcc atcagtaatc gccccagttc cggacggtca
     3901 agttttgtac cgcttatgtt ctcccgataa taactggcaa ttttatgccc ccgttcctgg
     3961 acgaactgtt ccagcatctc ttttgcccga tctgcaaact gatcttctgt tgatgccctt
     4021 aaataagctc tgatgaacat tttttcaccg cattatcgca tttaggttgt tgcatatatt
     4081 ctatcgcata taggttatgt cattaattgt atgaggtcac attttatatc tcgcatcagg
     4141 gtgtacccta tcgcgacagg caagcctgtt caaaaaacag gcttgccgta caataattct
     4201 ctatatccaa actgacccct atgagatcag tttggatatt cgaaattatt gtacgtttaa
     4261 cgcaccatca ttaatgctaa tgtatgcata cattagcatt aatggtgaag gaaaagaata
     4321 acaatggcag tcaacccact tcttttttgg atttcttaat tttcgtctga taaagccgct
     4381 ttacccgttc aatttctgcc agcgttgcgg accagtgtag ttcggcggca tgacgatatc
     4441 tggcagccat tttttccacc atctccggat tgctgtccgc gctgcggtgc tgacgcccct
     4501 tctccccaaa aacttcaaca accagccact ggcttccggt tctgccgaac aatgccttgc
     4561 acaaacgtct cacagtcagc ggggttaaat ctggatcggg atcataggca atggtagcaa
     4621 aaataaagcg cagcatttct gatgtcttgt aacgctttac gctggcagcc aggcgtgcgg
     4681 cacgttgcgg tgcttcccag cggtcgttac tgtaattagc cacttcagcc agataatcct
     4741 tttcaagttt ttcagccgtg tagcacctgc cgcctaaaaa aagccgcatg gcctccctgg
     4801 ttttatttct cacatatcat ccctcattaa tgctaatgta cgcatacatt agcattaaca
     4861 tactgacagt aacaagcggc ggatttacgt ccggataacg taaatgattg cggtatacag
     4921 gccaggcggt cagatttcca gccgtgcctg ttcctgcagc agttccagat gatatgcagc
     4981 aatagcgttt accacccctg ccgtcatccg tgcgtcggcg atcgctgagt gtgcctgccc
     5041 ttcccaggtc agtccggcct ggctggcagc acaggccagt gaaatcgtgc catagcggtt
     5101 ggtggcccca taatatcctg cagccagctc catcgcacaa tataccgtca tttcttccag
     5161 ccagtcagcc ggatcgctat gtgcggcagc agtctgtttc agaatgcgga tgtcgaaccg
     5221 ggcattaaag ataattactg gtcggtcccc gattgcatgc cgcagttgac gcgccacatc
     5281 tgtccatgat ggtgcgccgc agagagcctg ctcactgata ccatgcacgg cggcagcctg
     5341 cgccccgatg gcgaccgtgg gcttcagcct ggtttcaaat accacctgac cggccgcatc
     5401 ggtcaggccg atttccaggg cttccgcggt attatccagt cctgtcgttt cagtatccag
     5461 aaagagtggt gccagactca gccagtcata tgcctgccgt gccatccggc cactggtgct
     5521 gtttagtcgg gataaaacgg aaagccgctg gccagccagc tgttgtttac tcgtcagagg
     5581 gcgtttccca cgcatcggaa cacagtctgc aattcggtat acagcgaacc tgccaccata
     5641 agtattttta taccacttaa ccggctcagc accgggggct ggcttcattc tgaattcatc
     5701 gcgcagacgt cctttcgaga aacacctgtc ttcggcaatc gcctgcgcac gtctggtgac
     5761 cagctcatca ttattcatgg ttcttgcatc tccgtcagac gtttttatat ccggacagcc
     5821 cctctatggc cacttctgtg cagtatgcaa tactcgcagg atatacactt ttccccactg
     5881 gctgtcgacc tcataaacca gaacaaaatg cgggtgaata accagttccc gggtaccttc
     5941 cacacgaccg ttgcggcctt tatacggctg cccggccagg ctgctggctg aataaccaat
     6001 ccgttccagc catcaggttc tgctccgcaa tgtactcacg aatgcgcttc ctgtcctggc
     6061 tggccagcat ggtccagaat atctccatca tttaccggca accttcctgc tcatttcatc
     6121 ccgccatgct gcagcagagg attcgaccat gtcatgggga agcacatcgc cgtgctcgag
     6181 ttgctgacgt cctgccgcaa cctggtcgcg gaaccaggta ttatgctgcc gttcaacggt
     6241 ctgccgcata aaatcccgga ttaactgaga gccattacgg tccatgcttt ttgcagcggc
     6301 cataaaagca tccttcagtt cagcgtcgat tcttaaactc atattaacct gtgccattca
     6361 tcacctcgtc attaccacgt atatacacag tatataatta ttcagatatc gtcaccatga
     6421 tatgccgcgc cagcaacatg gaaggcggca ctccatgctg tatatggcag aggagtaaaa
     6481 ccgccgaatc ccggcgtaag ccggtacagt cgtccgaatg tcagtagttc tactcccccc
     6541 tgcgaggcca gtgctgacgc gggacatgga acgggtgctg gagcgaagtg gggaagctcc
     6601 ggtatactgg ccaaacctac acgcctcctt ccgtctccgg gaagatgctg gcttcgtacc
     6661 ctgcgtgcgt ctgatctgta gctggccgtg gcacggctac ggacagattc tgctccgcaa
     6721 cagtatcggc gtgcctgacc tcctgaaagt cccgtaacgc attttctcac ctcccatgcc
     6781 ctgacgctat tgagcgcgtt acagaacaat ttaagtcatt taacgagcct ccaacggctc
     6841 gatcgaaata aatcaaatcg taatcgtcaa cgatatatga gaggagaccc cgctcgccgc
     6901 aaatcgatga ccgaacgtag tgagcgaatc gacgaggaag cggaagagaa cctgtacgaa
     6961 aatggagcaa ttacgcccca ccgtcaagaa atgagctggc tctccacggc gcgccatcat
     7021 agacagatgt ggctctgcaa ctgcgccatg cgatcggaga ccgaccagta attatcttta
     7081 atgcccggtt cgacacccgc attctgaaac agactgctgc cgcacatagc gatccggctg
     7141 actggctgga agaactgaca gtatattgtg cgatggaact ggcagcagga tattatgggg
     7201 ccaccaaccg ctatggcacg atttctctgg cctgtgctgc cagcaggccg gactgacatg
     7261 ggaagggctg gcacactcag ctgatcgccg acacacggat gacgtcaggg gtggtaaacg
     7321 ctattactgc atatcatcag gaattgctgc agcttatgta ctgcgataac cctgaactca
     7381 actatgcaaa cctgaaatcc ctgcaaaaac agctggatga aatgtatacc tgcatccgta
     7441 ataatcagat gatgctccca aactacggaa aaatgcgccg gtatggtgaa ccggtttcaa
     7501 caacgtttgt agagtcgaca atcaacgaag tgatcgccag acgatgaccc aaaagcaaca
     7561 gatgcagtgg ggtagggaag gtacgcatta tttgttacag accagaaccg ctgtcctgaa
     7621 taattaactg caggacaaat tcgccagttg gtacccgggt tgcacgatag acgctcagag
     7681 tggaggaaag ttatccgcca tggcagcata actacttccc cagttttttc agctctccct
     7741 taccaatatc ctacgacgat cacatcgaaa aaaaacacgg aacaggcgaa cagaaatata
     7801 attaattatt tgagtttaaa ggaaaaaaga ggatacaagc tttttgttgt atttttattt
     7861 gtataaaccg acaaaaaaga ggccatactt cccgctttct ttgtctgttt acacggaaca
     7921 tttcaccact ggatacgcta atagatcgga tatgagcaca tattgcgccc gcctttcatg
     7981 cagagcataa aatgaacaag attcacgcta attattacct gcaagattct accgcacgcc
     8041 agatagttca acgcacaatg gggattattc cgtactcggt aaatgtgatg aacgagcatg
     8101 ggatgatcat cgcttccggg gaagcctcac gtttacatca acgccatgaa ggtgcggtac
     8161 tcgcattgac cgaaaaccgg gtcgtcgaaa ttgattccgc gaccgccaat aagcttaaag
     8221 gcgttaagcc cggaatcaat ttaccgatta attttcgcga ccagctgatt ggtgtactgg
     8281 gcattaccgg cgagcctggt gaagtgaagg catatgcaca gttggtgaag atggcttcgg
     8341 aattaatcat tgaacaaatg gctctcctcg agcaaaaaca gtgggataag cgttaccgcg
     8401 aagagctgat taatcagctg attttacgcg atccgccacc ggactcactg aatgcggtgg
     8461 tctcatggtt gggaattaat ctccagcttc cgcgggtggt ggtgattatg gaactgcaac
     8521 aacctgatcg cgacgcactg cataatctga tggagtattt tgaatatcgc gcccgcgacc
     8581 atttggtcac gttttccgac tttaatgatc tgataatctt aaaacctatc tcgctaaaaa
     8641 acggcgtctg ggataaatcg ttggaattaa gcgatctgca gagacttaaa cattacgccc
     8701 agtcctgtgg tttttcccga ctgattgtcg gcggctattt ctctggagaa aacggcctgc
     8761 atcgctcttt tcagaccgcg aaatccaccc agtccatggc gaaaagactg aagttgaagc
     8821 atcagttcat ttttcatgac gactatgcgc tggcatcatt attagatggg ctttctggta
     8881 cctggcaagc ggaagaatta tccaaaatct ggcgcaatct ggttgccaag gacaccaaag
     8941 gtatgctgca aaaaacgctg cagaattatt ttgatcataa ttgtgatctt tcgcaaacag
     9001 cgactggctt gcatattcat gtcaatacat tacgctatcg actgcagaga gtggaagata
     9061 ttacaggaat acaaatcaat aacttaaagc agttattctg gctgtatatc ggcatggagt
     9121 tacaacgctg aactgtattt ccctccaaat ttttcgccgt ctgacgtcgt atttttagcc
     9181 gatacacaca agtctaacgc ggctgctttc tccatactgc cccctgttat tttcatggag
     9241 aaagcagaag atggtttcaa taacagcaac tggcgccatc atcgcactcg tggtggcgat
     9301 cgttttaatt ttacgcaaag tctcgccggc ttacggcatg attgtcggtg cgctggtagg
     9361 cggtctggtc ggcggcgccg atctggtgca aaccgtgacg ctaatggtca ctggcgcgca
     9421 aggcattacc aatgccgtgc tgcgcattct cgcagcaggt gtactggccg gcgtgttgat
     9481 agaatccggc gcggcaaaca ccatcgctga aactgtggtg aaaaaagtgg gggaaagccg
     9541 ggcgctttta gccttagcta ttgcgacgat gattctgacc gccgtcggag tatttatcga
     9601 cgtggcggtt atcactgttg cgccgattgc cctgtctatt gcccgtcgtg ccggtttgtc
     9661 gaaactcgcc attctgctgg cgatgattgg cggtggtaaa gcgggtaatg ttatgtcgcc
     9721 taaccccaat gctatcgccg tttcggacac ctttcatgtt cccctcacct ccgtgatgat
     9781 gaccgggata atccccggtc tgttcggcct gatcgtggct tatttcctgg caaaatgtct
     9841 cagccagaaa ggcctgatgg tgatgcccga cgaaatgatt tcacacgaca accacggcaa
     9901 gcagccggga tttcttgccg ctatcagcgc gccgctcgtc gccattgtgc tgctggcgct
     9961 acgacctgtt gcgggtatcg tcatcgatcc gctgattgcc ctgcccgtcg gtgggttagc
    10021 cggtgcgatc cttatgggca ggtttaagca aagtaacgcc tttatgatgt ccgggcttac
    10081 gcgcatggcg ccggtagcca tcatgctgct cggtaccggt acgctcgccg ggattatcgc
    10141 caactcggaa ctgaaagatg tgctcattca tggactgacc tcttccggtc tgccttctta
    10201 tcttctggcc cctgtttccg gcgcgatcat gtccatggca acagcctcca ccacggcggg
    10261 aaccgctgtt gccgccgggg tattcagcca gacgctgctg gggcttggcg tatcagccct
    10321 tgccggttcc gccatgattc atgctggcgc aaccgtactt gatcacctgc ctcacggcag
    10381 cttcttccac gccacgggcg gcagtgtcgg catgctgatc catgaacggt taaaactgct
    10441 gccttatgaa acccttgtcg gttttgtggt cgccctgatt tccacactga tgtttggcgt
    10501 cttcggactt gccgggtaaa gacacgatga aaatagtcat tgcaccagat tcatttaaag
    10561 aaagtttatc ggccatgcag gtggccaccg ctattgagca gggatttcgt gaaatttatc
    10621 ccgaggccga gtatgtcaaa ttgccgatgg ctgacggcgg tgagggtacc gtcgaatcaa
    10681 tggttgaagc aaccgaaggc cattatctgt tccagaatgt caccggaccg ctggggacgc
    10741 cagtacaggc gcgctggggt atgctgggag atgggaaaac ggcgatcgtt gaaatggcct
    10801 ccgcatccgg actacatcat gttcctccag aatgccgtaa ccctatgctt accaccagtt
    10861 atggcacagg cgaactgatc cttgccgcac tggaccacgg cgttgagcga attattctcg
    10921 ggattggcgg tagcgccacc aatgacggcg gcgcaggtat gctacaggca cttggtgtgg
    10981 cgatgcttga tacaaacggg aaatcgcttc ctttcggcgg cggcgcgcta agcaacctgg
    11041 cgctcatcga tgcctcaggc tgcgacgctc gactgaaaaa cgtttctgtc accgtcgcct
    11101 gcgacgttaa caatccactc tgtggcgcaa caggagcgtc agccatcttc ggcccgcaaa
    11161 aaggagcaac accagaaatg gttgcctcct tagaagctgc gctgaccaac ttcggtatac
    11221 gacttcagga cgcaaccgga cgaaacgtga tgacgacgcc tggcgcaggt gccgctggcg
    11281 gcatgggcgc ggcgctgtta gggattctga atgcggagtt aaagccggga gtggagattg
    11341 ttattgacgc gctgcatctc gatagcctgg tcgcggatgc cagcctggta atcaccggtg
    11401 aaggtcgtct tgatagccag agcatcagtg gtaaaacacc gattggtgtc gcacgctgcg
    11461 ctaagcggta tcagaaaccg gtgatcgcac tggcgggcgg cttaacgacc gaccaccaga
    11521 tagtgcatga gcacgggctg gacgcggtgt tctccgtgct gacgcaaatc gccactctgc
    11581 cggaagcgct acaggatgcg gaatacaacc tccgcgtttc ggcacgcaat attgccagcg
    11641 tctggaaatt atcacagcag cagatgcgct aatcttcagt caggccggca agcagtagct
    11701 ggcctggata ttctttctca ccaggcccat gctaaaacag cctgcttcag tcatcccacg
    11761 caaggcacta gcatggcaaa aaaagagggg agtagtggat cccaaaagac ggttaagcgg
    11821 ttaaattgaa tggtcaataa gggggctgtt tgggaaacga atctatggtc actcccgttt
    11881 ttgcaacacc gattttgacg ataagtgggc ttgcttgaat ctatccggcg tctgaatggg
    11941 attttattcc cgcgcctcga tgagttccgc gcctgatgaa cctcaagaaa atatacggct
    12001 tcaatgagcc tttccgtttc acaggttcct caacaggccg gtgggccgtt catatcatca
    12061 ataccgtgat gctgatactc cgtacccacc ggaaccgcac ctgatgcggt tccggcttaa
    12121 gggctgtcag gactcgcttt tggctgccat caacaaaccg gaagagatgc cggattatct
    12181 cttccgtcaa ctcggtttta atcagacctg gcatgagtgg aagcgggacg aacagcacag
    12241 gcaacaacaa cgccggcccg gacactaccg gggcatgagt atgtaatgtc agggacagct
    12301 atacgccata aaaaaatgaa atagatggcc tctctataaa gaggccatga tatttactaa
    12361 ccctgttcgt taatacattc tataaagttt ttatgtaggc tatctatatt ttctcgggta
    12421 tgaatagctg atattgcaca acgtagaatt gcctttccct cttccactgt aggatacata
    12481 gcaacggttg tagcaaaacc acgatcatgc atttttttgc atatcgttat agctttttct
    12541 tcattgccaa ttaacaatcc acgtattggc attgtgtcct tgacccacga tattcagaca
    12601 gtcgttgtat cttaagttga cgttacccgc tgacgtcgat attcccttgg tgagagataa
    12661 cctaacgcac tatgcggatg ggtttcattg taatgctcga tcgccactgc aaggttatgc
    12721 aatgccgttc ttacattcgg tttcggcatg aacgcgatgt agtcttcttt catcgttttc
    12781 acgaaccttt ctgccattcc attgctctga gggctactga ctgccgtc
//
LOCUS       DAZGCQ010000135        12472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000135 DAZGCQ010000000
VERSION     DAZGCQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.135"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..778
                     /gene="csgD"
                     /locus_tag="ACHK07_004361"
     CDS             128..778
                     /gene="csgD"
                     /locus_tag="ACHK07_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO8104615.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQEHVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            783..1172
                     /gene="csgE"
                     /locus_tag="ACHK07_004362"
     CDS             783..1172
                     /gene="csgE"
                     /locus_tag="ACHK07_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO8104616.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1197..1613
                     /gene="csgF"
                     /locus_tag="ACHK07_004363"
     CDS             1197..1613
                     /gene="csgF"
                     /locus_tag="ACHK07_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO8104617.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1640..2473
                     /gene="csgG"
                     /locus_tag="ACHK07_004364"
     CDS             1640..2473
                     /gene="csgG"
                     /locus_tag="ACHK07_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO8104618.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2537..3028)
                     /gene="ycdZ"
                     /locus_tag="ACHK07_004365"
     CDS             complement(2537..3028)
                     /gene="ycdZ"
                     /locus_tag="ACHK07_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8104619.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACHK07_004366"
     CDS             complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACHK07_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO8104620.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACHK07_004367"
     CDS             complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACHK07_004367"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO8104621.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRALAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACHK07_004368"
     CDS             complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACHK07_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO8104622.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5672..5759
                     /locus_tag="ACHK07_004369"
     tRNA            5672..5759
                     /locus_tag="ACHK07_004369"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5706..5708,aa:Ser,seq:gga)
     gene            complement(5909..6751)
                     /locus_tag="ACHK07_004370"
     CDS             complement(5909..6751)
                     /locus_tag="ACHK07_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8104623.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMDKPDTAMKAITRNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6836..7033)
                     /locus_tag="ACHK07_004371"
     CDS             complement(6836..7033)
                     /locus_tag="ACHK07_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8104624.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(7045..7536)
                     /locus_tag="ACHK07_004372"
     CDS             complement(7045..7536)
                     /locus_tag="ACHK07_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8104625.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINVVCDNGY
                     SLRVVDKSNQQSTSTPPPFTALAGIRCSTAHITAKDNAWLYSLSHQTSDTGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPT
                     FDW"
     gene            complement(7533..7907)
                     /gene="cbtA"
                     /locus_tag="ACHK07_004373"
     CDS             complement(7533..7907)
                     /gene="cbtA"
                     /locus_tag="ACHK07_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO8104626.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYLEAK"
     gene            complement(7997..8350)
                     /locus_tag="ACHK07_004374"
     CDS             complement(7997..8350)
                     /locus_tag="ACHK07_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8104627.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            complement(8426..8647)
                     /locus_tag="ACHK07_004375"
     CDS             complement(8426..8647)
                     /locus_tag="ACHK07_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8104628.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8716..9192)
                     /locus_tag="ACHK07_004376"
     CDS             complement(8716..9192)
                     /locus_tag="ACHK07_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO8104629.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(9208..9687)
                     /locus_tag="ACHK07_004377"
     CDS             complement(9208..9687)
                     /locus_tag="ACHK07_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8104630.1"
                     /translation="MKIVSQNTTTLSSATAPGNNQPQLVATPVPDEQRISFWQQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGKTWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRIID"
     gene            complement(10026..10844)
                     /locus_tag="ACHK07_004378"
     CDS             complement(10026..10844)
                     /locus_tag="ACHK07_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8104631.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFICQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11171..>12472)
                     /locus_tag="ACHK07_004379"
     CDS             complement(11171..>12472)
                     /locus_tag="ACHK07_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8104632.1"
                     /translation="QNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSAS
                     GLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAYRA
                     EVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGAT
                     PESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGS
                     LGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGSLETGLPFSITDN
                     LMLEPQLQYTWQGLSLDDGQDNAGYVKFGHSSAQHVRVGFRLGSHNDMTFGEGTSSRA
                     PLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQ
                     AGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT         3061 a         3211 c         3158 g         3042 t
ORIGIN      
        1 atattttacc catttagggc tgatttatta ctacacacag cagtgcaaca tctgtcagta
       61 cttctggtgc ttctatttta gaggcagctg tcaggtgtgc gatcaataaa aaaagcgggg
      121 tttcatcatg tttaatgaag tccatagtat tcatggtcat acattattgt tgatcactaa
      181 accttctttg caggcgacgg ctctcttgca gcaccttaaa caatcgctgg caattacagg
      241 aaaattacat aatattcaac gttctctgga cgatatatct tcaggctcta ttattcttct
      301 ggatatgatg gaagcggata aaaagcttat ccattattgg caggatactt tgagcaggaa
      361 aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa gattacccgt accgcgacat
      421 tgaaaactgg cctcatatca acggcgtttt ttatgccatg gaggatcaag aacatgttgt
      481 caatgggttg caaggcgtcc tgcgcggcga atgctacttt acgcaaaagc ttgccagcta
      541 cctgattacg cattcgggta actatcgtta taacagcacg gaatcagccc tccttactca
      601 tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg tctaataacg agatcgctcg
      661 ttcgttgttc atcagcgaaa atacggttaa aacgcatctt tataatcttt tcaagaagat
      721 agccgtaaaa aaccggacac aagcggtttc ctgggcaaac gataacctca ggcgataaag
      781 ccatgaaacg ttatttacgc tggattgtgg cggcagaatt tctgttcgcc gcagggaatc
      841 ttcacgccgt tgaggtagaa gtcccgggat tgctaactga ccatactgtt tcatctattg
      901 gccatgattt ttaccgagcc tttagtgata aatgggaaag tgactatacg ggtaacttaa
      961 cgattaatga aaggcccagt gcacgatggg gaagttggat cactataacg gtcaatcagg
     1021 acgttatttt ccagactttt ttatttccgt tgaaaagaga cttcgagaaa actgtcgtct
     1081 ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca gataaatcag gcgttattaa
     1141 gtacggacga tttggcgcat gatgaattct aaataaaaaa ttgttcggag gctgcaatgc
     1201 gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc attaagttgg gctggaacca
     1261 tgactttcca gttccgtaat ccaaactttg gtggtaaccc aaataatggc gcttttttat
     1321 taaatagcgc tcaggcccaa aactcttata aagatccgag ctataacgat gactatggta
     1381 ttgaaacacc ctcagcgtta gataacttta ctcaggccat ccagtcacaa attttgggtg
     1441 ggctactgtc gaatattaat accggcaaac cgggccgcat ggtgaccaac gattatattg
     1501 tcgatattgc caaccgcgat ggtcaattgc agttgaacgt gacagatcgt aaaaccggac
     1561 aaacctcgac tatccaggtt tcgggtttac aaaataactc aaccgatttt taagccccag
     1621 cttcataagg aaaataatca tgcagcgctt atttcttttg gttgccgtca tgttactgag
     1681 cggatgctta accgccccgc ctaaagaagc cgccagaccg acattaatgc ctcgagctca
     1741 gagctacaaa gatttgaccc atctgccagc gccgacgggt aagatctttg tttcggtata
     1801 caacattcag gacgaaaccg ggcaatttaa accctacccg gcaagtaact tctccactgc
     1861 tgttccgcaa agcgccacgg caatgctggt cacggcattg aaagattctc gctggtttat
     1921 accgctggag cgccagggct tacaaaacct gcttaacgag cgcaagatta ttcgtgcggc
     1981 acaagaaaac ggcacggttg ccattaataa ccgaatcccg ctgcaatctt taacggcggc
     2041 aaatatcatg gttgaaggtt cgattatcgg ttatgaaagc aacgtcaaat ctggcggggt
     2101 tggggcaaga tattttggga tcggtgccga cacgcaatac cagctcgatc agattgccgt
     2161 gaacctgcgc gtcgtcaatg tgagtaccgg tgagatcctc tcctcggtga acactagtaa
     2221 gacgatactt tcctatgaag ttcaggccgg ggttttccgc tttattgact accagcgctt
     2281 gctggaaggg gaagtgggtt acacctcgaa cgaacccgtt atgctgtgcc tgatgtcggc
     2341 tatcgaaaca ggggtcattt tcctgattaa tgatggtatt gaccgtggtc tgtgggattt
     2401 gcagaacaaa gcagaacggc agaatgacat tctggtgaaa taccgccata tgtcggttcc
     2461 accggaatcc tgacagataa aaaagccgga attatcatcc ggcattttac tgcttaagcg
     2521 aaagcgtgag tgcccctcac gctttatttt tgatttcctg ctcacggtgc gccgtctttg
     2581 cactacgcgc cgccagccac aggccgctgt ttttcattgc gtaaccaaat atcagcccaa
     2641 gcgccagcga aggtaacacc agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc
     2701 ctataaacgt ccccggaaca aatgaaagca gcagctgttt ggcctgaata cacatcagaa
     2761 acgcgacaat accggttatg acataaccga gaatttccaa atgtggcgtc agtgcactac
     2821 cgtaaataat gaccatcgcc cacaccacgc cactaagcag ggttgcagcg gagatcgcca
     2881 gccctttcag cccaccttgc gggcaggcaa agtaggccgt acagcccagg aagcccgccc
     2941 agcttagtaa gcccagggaa acagccaccc atccccagat accggagaga atgcctgttg
     3001 tgattgcaat agagagaagt atgttcatgc cgcgcatgat agcagaaaac gcggccatga
     3061 atgagatcga gcacacattt tatgcaacag caaattatct gttgcattta tgagtgacgc
     3121 agatcacatt tactcttcag aatcttcttc cagctcgtcc cacattgcac taatggcatc
     3181 gcgggttagc ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc
     3241 aaggaacgca ccacaccagg gtaacagata ctcactgaac agtgtttcca gtgcttcgct
     3301 ctcatcttcc gttgactgat cttccagcca ggaagccgca agcagcaatg tgccgatgtg
     3361 atcggcaggc gtatccgcta atggcatccc tcgttcagaa agaaaagcgc gcacttccgc
     3421 ttccgttgcg tcctcaaccc atgcgctacg atatggcggc acggcacatt catcgccgat
     3481 aaacaacgca ttgtaatcgg cagagacttg cgtcatatca cagcttttct gcaaacgtgt
     3541 cagcaactca tcctgctcca gtggccagtt cgcagccagt ttcccctcac gaatcagggt
     3601 aaacagcggc accagtaaag gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg
     3661 acagaggata gaaaactcgt tcattaatat atttccatta ctagtaatta aaggtctgca
     3721 aattccgcaa tcggtgccat accgcgagat tcaaggaagt tcagtaagcg gcgcggagaa
     3781 acattcaaaa tgcgctctgg cggaaaatct accgcgtcga ggattttaag acactcttca
     3841 aattccccca tggtaaacgc ggtgtgagaa tccgagccta atgccaccca acctccagca
     3901 tcacgtaccg ctgcggctag cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa
     3961 aatgaggaat tattgatttc cagcgccacc tgatgtttcg cggctgcttc agcaacggct
     4021 ttaacatcta tctcatattt cggatttccg ggatggctta ttatatgcac attgccgctg
     4081 gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttat catgtggcgc aaaaaccggc
     4141 tcatgaaaac cggcaataat cagatccagc gaatcaaaca ttttgccgct gcagtcaatt
     4201 tcaccatcaa catttttaat gttagcttcg atgccgcgca ggatccctac cccatcaacc
     4261 actcgcggcc agatacgcat gttaatgaag tgccagtgat gtggcgcatc ttccatatcc
     4321 gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg ggcaatataa
     4381 tcactcaatg tgctatatgc atgcgtgctg gcaacggtat gcatatgaag gtcgacggga
     4441 tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct gatgctttat
     4501 tagtagccgc gtgcgcggtc gacttgcccg cagaccctct cccctttttc gagctgggca
     4561 atggtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc gacatgtggt
     4621 gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg ttcacgatta
     4681 aaaacatcca gcattgcgcc tttaactttc ccgctatcca gcgccgcgag caggtcatct
     4741 tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatccgg taatttttcg
     4801 agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa attaatcaat
     4861 acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct ttgcacgcca
     4921 ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgccaggt ttgcagactc
     4981 tgagcaactt tactgcccag tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga
     5041 tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa
     5101 cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc gcccataccg
     5161 gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg tgcctgtagc
     5221 ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag atcgcgccct
     5281 gccagcattt caacaggagg atgccagact aaagcataat cagcagagtc attatctccg
     5341 cttttccatg ctctgactct tgcctgagga atagctttgc gcagtgcctc aatccaccat
     5401 tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga aaagcgttgt
     5461 gcgaaattta ttcgcactta tcgttatgat ctacaaagac caccagcata acaaatccgt
     5521 ggtcggtggc aaaaaaagca gatttcgctt attaaaacca catattgatt gaagtttgaa
     5581 taaacgcgcg attttttaaa aaagtttgtt gacctcaggt catgatttcc ctaaattagc
     5641 gcccgttcca gcaagacagg aacgacaatt tggtgaggtg tccgagtggc tgaaggagca
     5701 cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg aatccccccc tcaccgccag
     5761 attacaagcg catgttattg tttaacatgc gcttttttat ttatccttcc agacagtatg
     5821 ccaccgggta tgccatctga aaccggttta tatgccccac gatcatatct tatcggacaa
     5881 tttccgatct ttgagcatac tggcaaagct atactgccag catgtcagga tagtgttttg
     5941 cgataatatc attcagtctg tcaaccagtt ctggcttcct gaacgtgatg tgcgcagagc
     6001 ccttcttaaa gtatttaata atgaacatct catcttcata gcattctttt ccctgaagag
     6061 accggatgtg atcgtccaga tgaatagtta tgttttccct gttgtcgggg atcggtttgc
     6121 cgctgaacag atgcagcatt ctttccaggt cggcaagtct atctgcctgt tgcccggtga
     6181 taagatgcaa tccccaccgg tcccacctca ccagattgtt gacgataatt ttattgccaa
     6241 atttacaggg gcaattggtt ttgtaattcc agctcaaccc tctgaagagg ttgatcactc
     6301 ctcgctcaaa cacttcatcc ttattctggt gtagctgctc aaacgtactc agaatattct
     6361 cttcactgat ttccggaaaa ttatcgtact ccagtgaccg gtaccatgta tcacgggcct
     6421 gtgcatccat taaggaaagc atcccggagc attgcatcag gtcacgccag atttcgcgat
     6481 cgagattgcg ggtgatagct ttcattgctg tatcaggttt atccatgagc cagcagccgc
     6541 agcgaaaatc ctgtttcatg gcccagtcca gtgcggtttt accgccaata ctttctgtca
     6601 ttgctgatat ttcagccagt tgccggataa gtgtgtcgat ttgcagcagt gtactctttc
     6661 gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc ccggtgacca
     6721 gttccggacg ggagattgtg atgtctgaca ttatcggttt ccctttgggt ttataaacga
     6781 aaacgccagc cctgttcagg ctgacgtcag tggaatgaag tctggtgagt gagattcacc
     6841 ggttctggtg cagaagctgc agttggcgca gggtacggat atcctccctg gcctgttcaa
     6901 tacagggcaa cagtgctgcc gaatccgttt tatcttcatc gttgtcaaag ataattcccg
     6961 attcgcagtc gatattgtcc tgcagccatg caatcagaat atccagcgct gtttccgtgg
     7021 ttaatgattt catgttgttt ctggttacca gtcgaaagtg gggaaacccg gcagacatcc
     7081 ggcactggca tccagatgaa tgagactgac gccataacgt cggatgagtg tgacaaccag
     7141 acggcggaac gtttttgaca gccccaggcg tttaagccgc agaaccgggt atgaccacgc
     7201 atcggtacgt aacagatatc cgctacctgt gaaatgaatc cattctgatt cgccggtgtc
     7261 actggtctga tgtgacagcg agtacagcca ggcgttgtcc tttgccgtga tatgcgcggt
     7321 actgcagcgt atgccagcaa gggccgtaaa cggtggtggt gtactggttg actgttggtt
     7381 ggatttatcg accacgcgga gtgaataacc gttatcgcag accacgttaa tcagttcagc
     7441 gagattaaca ccatcaatgt caacgacaat gcgccccatg ttcagtgcct gtacgttaac
     7501 gctgtcggct tccagtgtca gggccagttt catcatttcg cctccagata cttacccagg
     7561 gtaatgttat ttaccgttct gtaattgtcg cgggtcatca gacctgttgc ccggcgtgcc
     7621 cggagaatat caatgctgtt gattaactgc gacgaagttc ctgcgctgaa tcccggctgg
     7681 tcggtacgca ccagcgcgta tttttccacg agaaaattca ccgcatcaca cagtgaaatg
     7741 cctgcctcaa tatgctgctc aatcacacgt tcatcagcga acggtgtgtc attcagcgta
     7801 aggccatagt gctggtccag cagtcgggtg agcagtgtct gccagatttc aacaggagat
     7861 gggcgtgaac tggccgactg cccgggtaat acaggtaatg ttttcatact gaagattttc
     7921 ctgatatgca aagatgaaaa atgggaaagt ggtgtggtga aaacaccagg ccggagcaga
     7981 gggttattct ggagagttac tttttcgttt cgggcgtcgg ataaacagcc atataaacgt
     8041 agccacaact gccgagggtg tcggcttcgc aggtcagccc tctggcatac agcgtgacgg
     8101 tatgctgatg gcggggattc agttcaccgc tggtgagcat gagttccagt tgtttcatca
     8161 gcagcggaaa ggcctggtcc aggtggtacg catctgcatc gctgaacaag cctctgatac
     8221 cggcgcggtc ggcaaggtaa tgcaaccggt taccctcctg caccagacgg gcgccgaaac
     8281 agggcgtcac ggtgcagggc agcccccacc aggggcggtc gtgattgccg tcgggaagtg
     8341 tgtctgacac gataaaatcc tcacagaaaa tcggttaaga atggtccggt ggtggcgata
     8401 attctgctca tcagaatgcc ctgactcagt tcagggtgac gctcatcagc cggacatacg
     8461 ggccaaaact gtccttacgg cgttcagcaa acacggccag cacaccggga atatcctgta
     8521 cttcacgacc ggtatacgcc ccggcactgc cgtgccagcg gtacttaccg gtgcagaacg
     8581 gaaaaagacg ggaggccgga tgttgttggt gaatacgcat ggcttcacca cgggtgatga
     8641 ttttcatagt gtgatacctc tgaagacaga agataaaagt gaaaacaggt gtgatgtggt
     8701 tgtgacggtg acgggttaaa gcagaccgtg ttccgcaaag gagaaaacct ggctgccacc
     8761 gactatcaga tggtccggca cccggatatc caccaggccc agtgcctgta ccagacgttc
     8821 cgtgataagg cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgtgccag
     8881 caccacggct gcggcattgt ggtacagggc gcgtttaatc acctcccggg gatggacttc
     8941 cgtgcggttg atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttatt
     9001 cagatacagc acccggaatt cttcacgctc cagtcccgcc atgttcagaa tcagccattc
     9061 ccgtgccgca cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt
     9121 tttcagggcc cgcagaatga gactgcgctc gccgggcgtc atctctccgg gcagaaagga
     9181 aagctgctgc attgtccctg cctccattca gtcaatgatg cgcataatgg cgttgcattc
     9241 cggatgttgc agggcgtaat cccgcagccg gtaatagtgg accgtcatgg cataacactc
     9301 cgtacgacag gcgtgatgac tgtacgtcat cagacaggca gcaatgccgg cagcttccgg
     9361 actcatttca gcgcggttac cgttcatggc attgaacagt acccaggttt ttccatcatc
     9421 ttcatccggt tcgggtgcca taaatgcgcc accgttgttc agggtgtaca gattccagat
     9481 accaccgcag tagtcttcgc acagacggtc catccagccg aagacacggg gctccagggt
     9541 cacccactgt ggaatgaggc caaaatgctg ctgccagaag ctgatacgtt gttcatcagg
     9601 gacgggagta gcaaccagtt ggggctggtt attccctggt gctgtggctg aagaaagagt
     9661 agttgtgttc tgagaaacga ttttcatgag tgaaatcctt acaatgtgta taaatgagag
     9721 gaaataaagg ccgggggctg gcaacatcac gttcatcact gaccgtgaga cccggtaata
     9781 tcagggatga atgatattcg gctgaaattc cggacagccg tacgcgtaac cgttccggtc
     9841 acagacggat ggccagatgc cattgcctgt actcagtctg cacagactga gttctccggg
     9901 acggtaaagc agacgggtac accaggtgca caggtcatca tcagaaatgg aacgacaccc
     9961 cggcagcgcc tccggggcgt cgggtgattc tggtacctga atcacgcctt cagggatatg
    10021 ttttatcagc gcatgctctc cagcagcgtt tctgccatta cccacaatgc gcggttgagc
    10081 ttaatatcgg tgtcgatgct gtgaatggca cgggtgtgga tacgttttcc ttttgcactg
    10141 cggccggaaa tcccgccttt cagcatattc tcctgtatgg tctgataagc actccacagg
    10201 tccttaccgt aatcctcccg gcgtcgcggt gtcagaatat cagcggtggt gacgggctga
    10261 tgctcattac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc
    10321 ggcagaatca gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa aacgcccacc
    10381 acctcgtaag ccccttcaat aactttctcc actacgtctc cccggtgtgg aacacgcact
    10441 tcccccagag actgaccaca gacgcagccg ttctggcaga tgaacctgaa gtaacccggc
    10501 agcatctggt agctggaggt accgtcatgg gagttgagca gaataatttc agggacatgt
    10561 tgtccgttta tctctccggc ccgccgcaga cgcagcatgt gtttggtata ttcccggcgg
    10621 cccgggtcac gtacgcgggt ctggcaggcg aagaatggct gaaagccttc ccgctgcagg
    10681 ctttccagca cggtgatggt gggaatccag gtatagttct cgcttctgga tgtgtgtttc
    10741 tcttccccga atacgctggg gacgacctgc atcagttctt cacgagttaa ggggcggtca
    10801 cggcgtatct ggttcgcata accaaaacga ctggctaatc gcatgattta ttccttatga
    10861 atgagttagc ggtataaatg aaaaagccgc gtctcccgga gaagacgcgg cctgatgggg
    10921 gaaatgaatg acgtttattg tctgagaagc ccttcactgg cgagctgcgt atttttatgg
    10981 aaggagatca cccaataacc ccatcgggat cgggtaataa ccggccagac tgtgatcgat
    11041 tcagatgcca tttggcagca aaagagcctt cggcaataaa cgtgctgtac aacccggcat
    11101 atctgttgct ccctgaaagt aaaaagcccc ggtcatgatg accggggcct gaaggggagt
    11161 gacctgatta tcagaaagtc acattcagcg tggcctgacc gttatagcct tcagcgctgc
    11221 tgccgctgac gctgtgggca taaccggcct gaacgcccag ggtgatattt tcccggacac
    11281 gggcttccag tccggcctgc aggtccagtg aagtgccatt ctgtgaggga gagaacgtca
    11341 tcccgctgcc tgcggtggag gtccccacac gcatatcacc ccgggagctg aaggtgcgga
    11401 taacggaagg ctgtacccac cagttcaccg gcagttcacg cacactgtgt tttgcactgt
    11461 cacgcagggg ggcacgggat gaggtgcctt caccaaaggt catatcgttg tggctgccca
    11521 gacggaaacc gacacgcaca tgttgtgcac tgctatgccc gaacttcaca taaccggcgt
    11581 tatcctggcc gtcatccagg gaaagtccct gccaggtgta ctgcagttgt ggctccagca
    11641 tcaggttgtc agtgatactg aagggcagac cggtttccag tgagcccagc cagccccggc
    11701 cccgtgcgcg gaagtcgtta ttgcccgatg acgctttcat actgtggcgg gttccctgtg
    11761 ccacaatgtc agcccacagg ccggaggagg tgtgtgtcag gttcatatat ccgcccaggc
    11821 tgccggcatc atcccggaca gtgccggcgc gggagccgtc atcatcctta acatcaacgg
    11881 aagaatggcc agcagcacca tatacccccg cggtcacaga cataccggca acctctgttc
    11941 tcatcaggtc accctccaga cggacgaatc catagctgcc gctgctttcc ggcgtggccc
    12001 cacgggcaat accgccattg ttatcgtgac cgagatgacc gccctgaatg ctgagacgga
    12061 cgctgttgtt ttcaccattt acaccggtct gatggctgcg ggagcctgcc acaatccggt
    12121 catagtccat tgcctgtgtc agcatggagg catacagggg gacttctgca cgataagcat
    12181 tttcactgcg cagataccag ctctcatcac tgtcccggtt gagggagtag ttaaaggcac
    12241 cggcctgcag cctgttcccc tggacaaagg ccccttcctc cgtggtggca ccgttaatgg
    12301 cttcaaccac ctgaataccc ttaccgctgg tcgccagccc cgacgcactg ttgccggcgt
    12361 tcaccaggtt caggatggtt tttccggttg ccctgccgcc gtcaatgacc agtctgtcag
    12421 cattgttctg tgccatatcc gggcgtacac gcaggctgat ggtgccattc tg
//
LOCUS       DAZGCQ010000136        12355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000136 DAZGCQ010000000
VERSION     DAZGCQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.136"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..2261
                     /locus_tag="ACHK07_004380"
     CDS             9..2261
                     /locus_tag="ACHK07_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO8104633.1"
                     /translation="MFSIKPGPRNLPIDNPTLLSWNITDGDLNSKLNTLEYLNCVTNI
                     INACGVYPQDLKDREIISTFHAEKIINDLLKNDYKISLFPDTTYRELNKAAQRSITAP
                     DRIGEGKTWIYQRDTMNEREDNKGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIEGGIPGYWQGQVLIPFIIDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWEN
                     LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKAVMNYMEYLVTSQLDEQQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGKYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGK
                     TGEPPNYLYL"
     gene            2596..3573
                     /gene="glaH"
                     /locus_tag="ACHK07_004381"
     CDS             2596..3573
                     /gene="glaH"
                     /locus_tag="ACHK07_004381"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO8104634.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLAQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3592..4860
                     /gene="lhgO"
                     /locus_tag="ACHK07_004382"
     CDS             3592..4860
                     /gene="lhgO"
                     /locus_tag="ACHK07_004382"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO8104635.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARNVDALHAAFNQ"
     gene            4883..6331
                     /gene="gabD"
                     /locus_tag="ACHK07_004383"
     CDS             4883..6331
                     /gene="gabD"
                     /locus_tag="ACHK07_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8104636.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGD
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6345..7625
                     /gene="gabT"
                     /locus_tag="ACHK07_004384"
     CDS             6345..7625
                     /gene="gabT"
                     /locus_tag="ACHK07_004384"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO8104637.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPNAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            8043..9443
                     /gene="gabP"
                     /locus_tag="ACHK07_004385"
     CDS             8043..9443
                     /gene="gabP"
                     /locus_tag="ACHK07_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO8104638.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9464..10126
                     /gene="csiR"
                     /locus_tag="ACHK07_004386"
     CDS             9464..10126
                     /gene="csiR"
                     /locus_tag="ACHK07_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO8104639.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMNLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEARDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMCQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10127..10576)
                     /gene="kbp"
                     /locus_tag="ACHK07_004387"
     CDS             complement(10127..10576)
                     /gene="kbp"
                     /locus_tag="ACHK07_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO8104640.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10660..10818)
                     /gene="yqaE"
                     /locus_tag="ACHK07_004388"
     CDS             complement(10660..10818)
                     /gene="yqaE"
                     /locus_tag="ACHK07_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8104641.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            11001..11300
                     /gene="ygaV"
                     /locus_tag="ACHK07_004389"
     CDS             11001..11300
                     /gene="ygaV"
                     /locus_tag="ACHK07_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8104642.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11310..11828
                     /gene="ygaP"
                     /locus_tag="ACHK07_004390"
     CDS             11310..11828
                     /gene="ygaP"
                     /locus_tag="ACHK07_004390"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO8104643.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11881..12285)
                     /gene="stpA"
                     /locus_tag="ACHK07_004391"
     CDS             complement(11881..12285)
                     /gene="stpA"
                     /locus_tag="ACHK07_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO8104644.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3083 a         3066 c         3195 g         3011 t
ORIGIN      
        1 acgttattat gttttctata aaaccaggac caagaaattt acctatcgac aaccccacat
       61 tgttatcatg gaacattact gacggggatc taaattccaa attaaataca ttagaatatc
      121 taaactgtgt aacaaatatt attaatgctt gtggagttta tcctcaggat ttaaaagaca
      181 gagaaattat atcaactttt cacgcagaaa aaatcattaa tgatctgtta aaaaacgatt
      241 ataaaatttc cctttttcca gatacaactt accgtgaact gaataaagct gcacagcgta
      301 gtattacagc gccagacagg ataggagaag gaaaaacatg gatttatcaa agagatacaa
      361 tgaatgaaag agaagataac aagggggttc accagtatgg tcctgctgaa catttcaccc
      421 acattatatc tgacaaaccc tccccaaaag ataaatatgt tgcatatgct attaacattc
      481 ctgactatga gctggcagcc gatgtatata atattaacgt gacgtcacct tccggacagc
      541 aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt gagcgtaaat
      601 ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa cctggcgaag
      661 cgattcttca tgcttttaat gccacctacc agcaaatcag agaaaatatg tctgaatttg
      721 cacgttgcca ttatgggtat atacaaatcc ctccagtgac aactttccgt gccgacggac
      781 cagaaactcc cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc gaagatcttt
      841 gtaccatcca caatccaatg ggagatttgc aggattttat cgcattggtt aaagatgcta
      901 aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga atcgggggga
      961 gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc agtaaagata
     1021 tagaaggtgg tatccctggc tattggcaag gtcaggtttt gattccattt attatcgatc
     1081 cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact gcaaaagact
     1141 tcgaagctag taaagataat atttctaagg atgaatggga aaacctccat gcattaaaag
     1201 aaaagcgttt aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt attatgctgc
     1261 aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt tatgatgcgg
     1321 caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt aaaaattata
     1381 atgagcgatt acacaatact aacttattta acccaaaata tcatgaaaaa gccgttatga
     1441 attacatgga atatctggta actagtcagt tggatgaaca acaaatgtca tcgctgcttt
     1501 atgaaagaga tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagctt
     1561 tttcatttgg tggcgatctc caaacccttg catcaaaacc gggttcaaca atctcaagca
     1621 tcccatcaaa aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc
     1681 ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg
     1741 ccgctctccc gttcagcagg cctttagttt actgggatgg ccaagtatta aaatcaacga
     1801 ctgaaattaa aaatgatgat gggtcgacgc gtgtcggcgg tgaggcgtgg cttaataaag
     1861 gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag
     1921 gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg
     1981 gggattctgt gaacattaat cactctcctc atgatggact agttataata aataaaggaa
     2041 acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaaaatat aaaaacatgg
     2101 ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc cctggtaaag
     2161 tatttacgct tagaggcgga actctaaata tcaatattcc tgggcgtagc gctcttcttt
     2221 tagggaaaac aggagaaccg ccgaactatc tctatttgta atttaatgtt atatctgccc
     2281 cgctaaaacg gggcagataa tatgtttagt ttactaacgg tcattttgca ttgaagccat
     2341 ttactgtttt ttatcgacca gataatctgt tctctgatgg taactccccc taacctgttg
     2401 ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat
     2461 ttgttgtttt tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa
     2521 tgtagatatt tttagattat ggctacgaaa tgagcatcac gcactctccc tgaaacctca
     2581 agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa acaacgctgt cgattcaggc
     2641 caggactata gcggattcac cctcacccca tcggcgcaat ccccgcgtct gctggaactc
     2701 acctttaccg aacagacgac caaacagttc ctcgcgcagg ttgccgagtg gcccgtgcag
     2761 gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca aaattcttga tgatctgtgt
     2821 gcgaatcagc tgcaaccgtt gctgttgaag accctgctaa accgcgctga aggtgcgttg
     2881 ttgatcaatg cggtgggggt cgatgatgtg aagcaagcgg atgagatggt gaagctggca
     2941 acggcggtgg cgcatctgat tggtcgctcc aatttcgacg cgatgagcgg tcagtattac
     3001 gcgcgttttg tagtgaaaaa tgtcgataac tcagacagct atctgcgtca gccgcaccgc
     3061 gtgatggagc tgcacaacga cggcacctac gtggaagaga tcaccgacta tgtgctgatg
     3121 atgaaaatcg acgagcaaaa catgcagggc ggaaattcgt tgctgctgca tctcgatgac
     3181 tgggaacatc tcgaccacta tttccgccac ccgctggcgc gccgcccaat gcgctttgcc
     3241 gcgccgccga gtaaaaacgt cagcaaagat gttttccatc cggtgttcga cgttgatcaa
     3301 cagggtcgcc cggtgatgcg ttatatcgac caattcgtcc agccaaaaga ttttgaagaa
     3361 ggcgtgtggt tgagcgagct ttccgatgcc attgaaacca gcaaaggcat tctttctgtg
     3421 cccgttcccg ttggcaaatt cctgttgatt aacaacctgt tctggctgca cggtcgcgac
     3481 cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct
     3541 tacgccactc accactacca gacgcatcag tagcgcaaag gaattgagcg gatgtatgat
     3601 tttgtgatta ttggcggtgg catcatcggc atgtcgaccg ccatgcaact gattgatgtc
     3661 tacccggacg cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg
     3721 gggcacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggca
     3781 cagttttgcc tggcggggaa ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc
     3841 tacgacaact gcggcaagat gctggtcgcc acgtccgatc tcgaaatgga acggatgcgc
     3901 gcgttgtggg aacggacggc ggcgaacggc atcgagcgcg agtggttaaa cgccgatgaa
     3961 ctgcgcgagc gcgaaccgaa tatcaccggg cttggcggta tttttgtgcc gtccagcggc
     4021 attgtcagct accgcgaagt cacggcggcg atggcaaaaa tcttccaggc cagaggcggc
     4081 gagattatct ataacgccga agtcagcggc cttagtgagc ataaaaacgg cgtggtgata
     4141 cgtacccgtc agggtggcga atacgaagcg tcaacgctga ttagctgttc cgggctgatg
     4201 gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttattatctg cccgttccgt
     4261 ggcgagtatt tccgcctggc gccggagcat aaccagattg ttaaccacct gatttacccc
     4321 atccccgacc cggcaatgcc gtttttaggc gttcatctca cccgcatgat cgacggtagc
     4381 gtcaccgttg ggccaaacgc agtgctggcg ttcaaacgcg aaggctatcg caagcgcgac
     4441 ttctcgttta gcgacacgct ggagatttta ggctcgtcgg ggattcgccg ggtgctgcaa
     4501 aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg
     4561 cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc
     4621 ggtgtgcggg cgcaggcggt atcgccggac ggcaagctga ttgacgattt tctgtttgtc
     4681 accaccccgc gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt
     4741 cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc
     4801 ggacgcacgc tgcgagcggc acgtaatgtg gatgccttac acgccgcatt taatcaataa
     4861 cctttgaaaa caggatgtag cgatgaaact gaacgacagc aacttattcc gccaacaggc
     4921 gttgattaac ggggaatggc tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc
     4981 ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa cccgcgccgc
     5041 tatcgacgcc gccaaccgcg ccctgcccgc atggcgcgcg ctcaccgcca aagaacgcgc
     5101 caacattctg cgcaactggt tcaatttgat gatggagcat caggacgatt tagcgcgcct
     5161 gatgaccctc gaacagggta aaccgctggc tgaagccaaa ggcgaaatca gctacgccgc
     5221 ctcttttatt gagtggtttg ccgaagaagg caaacgcatt tatggcgaca ccattcccgg
     5281 tcatcaggcc gataagcgcc tgattgtgat caagcagccg attggcgtta ccgcggctat
     5341 cacgccgtgg aacttcccgg cggcgatgat tacccgtaaa gccggcccgg cgctggcggc
     5401 aggctgcacc atggtactga agcccgccag tcagacgccg ttctctgcgc tggcgctggc
     5461 ggagctggcg atccgcgcgg gcattccggc tggcgtgttt aacgtggtca ccggttcggc
     5521 gggcgcggtg ggtgacgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg
     5581 ttcgaccgaa attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc
     5641 gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc
     5701 cgtggaaggc gcactggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa
     5761 ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt
     5821 gagcaaactg catatcggcg acgggctgga taaaggcgtc accatcgggc cgctgatcga
     5881 tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaaa aaggcgcgcg
     5941 cgtagtttgc ggtggtaaag cacacgaacg cggcggtaac ttcttccagc cgaccattct
     6001 ggtggacgtt ccagccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc
     6061 gctgttccgt tttaaagatg aagccgatgt gatcgcgcag gccaatgaca ccgagtttgg
     6121 ccttgccgcc tatttctacg cccgtgattt aagtcgcgtc ttccgcgtgg gcgaggcgct
     6181 ggagtacggc atcgtcggca tcaataccgg gattatttcc aatgaagtgg ccccgttcgg
     6241 cggcattaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt
     6301 agaaatcaaa tatatgtgca tcggtcttta actggagaac gcgaatgagc agcaataaag
     6361 agttaatgca gcgccgcagt caggcagttc ctcgtggcgt tgggcaaatt caccccattt
     6421 tcgctgatcg cgcggaaaac tgccgggtgt gggacgttga aggccgtgag tatcttgatt
     6481 tcgcgggcgg cattgcggtg ctcaataccg ggcacctgca tccgaaagtg gttgcggcgg
     6541 tggaagcgca gttgaaaaaa ttgtcgcaca cctgcttcca ggtgctggct tacgagccgt
     6601 atctggagct gtgcgagatt atgaatcaga aggtgccggg cgattttgcc aagaaaacgc
     6661 tgctggtcac cacgggttcc gaagcggtgg aaaacgcggt gaaaatcgcc cgcgctgcca
     6721 ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg cactacacgc
     6781 tggcgctgac cggcaaggtg aatccgtact ctgcgggcat gggcctgatg ccagggcacg
     6841 tttatcgcgc gctttatcct tgcccgctgc acggcatcag tgaagatgac gctatcgcca
     6901 gcatccaccg gatcttcaaa aatgacgccg cgccggaaga tatcgccgcc atcgtgattg
     6961 agccggttca gggcgaaggc ggtttctacg ccgcgacgcc tgcgtttatg cagcgtttac
     7021 gcgcgctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg
     7081 ggcgtaccgg cacgctgttt gcgatggagc aaatgggcgt ggcaccggat ctcaccacct
     7141 ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc gccgaagtga
     7201 tggatgccgt cgctccaggc gggctgggcg gcacctatgc gggtaacccg attgcctgcg
     7261 tggctgcgct ggaagtgttg aaggtgtttg agcaggaaaa tctgctgcaa aaagccaacg
     7321 atctggggca gaagttgaaa gacggattgc tggcgatcgc cgaaaaacac accgagatcg
     7381 gcgacgtacg cgggctgggg gcgatgatcg ccatcgagct gtttgaagac ggcgatcaca
     7441 gtaaaccgaa cgccaaactc accgccgaga tcgtggcgcg cgcccgcgat aagggcctga
     7501 ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg ctcacaattg
     7561 aagatgctca gattcgtcag ggtctggaga tcatcggcca gtgttttgct gaggcgaagc
     7621 agtagcaccg cccccatgcc ggaggcgacg ctgcgcgtct tgtccggcct acgagagtgt
     7681 aggtcggata aggcgtttac gccgcatccg acaatcggcg caccatgccg gaggcgacgc
     7741 ttacgcgtct tgtccggcct acggaaatgt aggtcggata aggcgtttgc gccgcatccg
     7801 acaatcggcg cacccatgcc ggaggcgacg ctttgcgtct tgtccggcct acggggattc
     7861 tcgtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aagaaatcta
     7921 tgttggccgg gtaaggcgga gccgctctcc ggcaaaaaga atcaataaca attaaacgcg
     7981 tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
     8041 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaagtca cgccacgtca
     8101 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
     8161 tcgccgaagc gggcccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta
     8221 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
     8281 cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg ctgtactggt
     8341 ggttttgggt actggttatc ccactggaag ccaacatcgc cgccatgatc ctgcactcgt
     8401 gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaactggca
     8461 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag
     8521 tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt ttttacccgt
     8581 atgccgacgt gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggtt
     8641 tcggtgcggt attaagtgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
     8701 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc gccactaact
     8761 cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa
     8821 taccgtggaa tatgccgggg ctgaaagccg ttggttctta tcgctcggtt ctggaattgc
     8881 tcaatattcc ccatgcgaaa ttaatcatgg actgcgtgat attactttcc gtaaccagtt
     8941 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggtg
     9001 atgcgcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac
     9061 tctccaccgg cgcggcattt ttaacggtgg tggtgaacta ttacgcacct gcgaaagtgt
     9121 ttaaattcct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
     9181 tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcctgcgca
     9241 tgtggcttta cccgtggctc acctggctgg tcatcggctt tattaccttt gtgttggtgg
     9301 tgatgctatt ccgcccggcg caacagttag aagtgatctc cactggctta ttagcgatag
     9361 ggattatctg taccgtgcca attatggctc gctggaaaaa gctggtattg tggcaaaaaa
     9421 cacccgttca taatacgcgc tgataatgat caggagtcac accatgacca ttacgtctct
     9481 ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac cggatgaaaa
     9541 attacgaatg aatttgctga catcgcgtta tgcacttggc gttgggccgt tacgggaagc
     9601 tctttcgcaa ctggtggcgg aacggctggt cacggtggtg aatcaaaaag ggtatcgggt
     9661 ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc
     9721 gatgttagtg agtctggcga ttgcccgcgg tggcgatgag tgggaggcag acgttctcgc
     9781 aaaagcgcat ctgttgagta agcttgaggc ccgtgacgcc agcgagaaaa tgcttgatga
     9841 gtgggatctg cgtcatcagg cgtttcatac ggcaattgtg gcgggctgtg gttctcacta
     9901 tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgctatcgat ttatctggct
     9961 gcgacaaaca gtgctttcgg tggaaatgct ggaggataaa cacgatcagc accagaccct
    10021 gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgt gccagcattt
    10081 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttcaggaa
    10141 tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt ttattcgctt
    10201 cgaagatttt attgtacaga ttggcgttac cgtagacctg tttggaaatg gcactcagag
    10261 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
    10321 gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
    10381 cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
    10441 ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat
    10501 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
    10561 aattgaacag ccccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
    10621 ggaacgcggc gcgttaacgc cgcggggatg agcacaggtt taatcgcggg tttgcaccca
    10681 gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc aacagaatat taataatgaa
    10741 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
    10801 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
    10861 ttttatctct taagcgtagt aattttagcg gaggctgcca gtttcagctt acgcttttac
    10921 tctcatttac gctgctgatg ctggtatatg ttgcattttt attttagagt ttacttaatt
    10981 tagctaacac ttaaatgact atgactgaac tcgcacaatt acaggccagt gccgaacagg
    11041 cagcggcctt attgaaagca atgagccacc ctaaacggtt gctgattctg tgcatgctta
    11101 gcggttcacc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
    11161 cgacatcaca gcatctcgcc cgtatgcggg acgaagggct tatcgacagc caacgggatg
    11221 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
    11281 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
    11341 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgacgct gatgaatatc
    11401 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtgctggaa cagtcaggtc
    11461 tcccggctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
    11521 gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatcttt ttactcgaag
    11581 atgggattga tggctggaaa aaagcaggat tgccagtagc ggtaaataaa tctcaaccct
    11641 tgccgttaat gcgccaggtg cagatcgctg cgggtggttt aatattaatc ggcgttgtac
    11701 tgggttatac cgtaaatagc ggtttcttct tattaagcgg ctttgttggt gccgggttac
    11761 tgtttgcagg aattagcggt ttttgtggga tggcaaggtt gttagataag atgccgtgga
    11821 accaatgagc ttgagaagcg acgccggacg cgccatagca gcgacatccg gcctcagtaa
    11881 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcga ttggcttcgg
    11941 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcggtga atttatattt
    12001 cgccggacgc ggctggcgtt ttttaccagc acgtggtgca gcagcagagc tattacccaa
    12061 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
    12121 ctggcgctct gccagttcac gctgctgctg ttcttcttct tcacgtcttt ctttagtgac
    12181 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
    12241 acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
    12301 ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa gcgatcactt attat
//
LOCUS       DAZGCQ010000137        11914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000137 DAZGCQ010000000
VERSION     DAZGCQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.137"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..469
                     /locus_tag="ACHK07_004392"
     CDS             <1..469
                     /locus_tag="ACHK07_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8104645.1"
                     /translation="APEILDQFLDEKEGNHTTAYRDGAGIWTICRGATQVDGKPVVPG
                     MKLSKEKCDQVNAIERDKVLAWVAKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFY
                     KRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            760..891
                     /locus_tag="ACHK07_004393"
     CDS             760..891
                     /locus_tag="ACHK07_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104646.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            855..1009
                     /locus_tag="ACHK07_004394"
                     /pseudo
     CDS             855..1009
                     /locus_tag="ACHK07_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1074..1535
                     /locus_tag="ACHK07_004395"
     CDS             1074..1535
                     /locus_tag="ACHK07_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8104647.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            1835..2641
                     /locus_tag="ACHK07_004396"
     CDS             1835..2641
                     /locus_tag="ACHK07_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO8104648.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2622..4328
                     /locus_tag="ACHK07_004397"
     CDS             2622..4328
                     /locus_tag="ACHK07_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO8104649.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGACGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4328..6472
                     /locus_tag="ACHK07_004398"
     CDS             4328..6472
                     /locus_tag="ACHK07_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO8104650.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6630..7637
                     /locus_tag="ACHK07_004399"
     CDS             6630..7637
                     /locus_tag="ACHK07_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104651.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEYLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            7661..8875
                     /locus_tag="ACHK07_004400"
     CDS             7661..8875
                     /locus_tag="ACHK07_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO8104652.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            8931..9320
                     /locus_tag="ACHK07_004401"
     CDS             8931..9320
                     /locus_tag="ACHK07_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104653.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            9370..9831
                     /locus_tag="ACHK07_004402"
     CDS             9370..9831
                     /locus_tag="ACHK07_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104654.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            9815..10378
                     /locus_tag="ACHK07_004403"
     CDS             9815..10378
                     /locus_tag="ACHK07_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104655.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            10378..11028
                     /locus_tag="ACHK07_004404"
     CDS             10378..11028
                     /locus_tag="ACHK07_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO8104656.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            11025..>11914
                     /locus_tag="ACHK07_004405"
     CDS             11025..>11914
                     /locus_tag="ACHK07_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO8104657.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGE"
BASE COUNT         3049 a         2768 c         3412 g         2685 t
ORIGIN      
        1 tgcgcctgaa atcctcgacc agtttcttga cgaaaaagaa ggtaaccaca ccacggcata
       61 ccgtgatggt gcgggtatct ggaccatctg ccgtggtgcc acccaggtgg atggtaagcc
      121 tgtcgtcccc ggcatgaagt tgtcgaagga aaaatgcgac caggttaacg ccattgaacg
      181 tgataaggtg ctggcatggg tggcgaaaaa catcagagtg ccactgaccg aacctcagaa
      241 agcggggatc gcgtcattct gtccttacaa cattggcccc ggtaagtgtt tcccgtcgac
      301 gttttataaa cgaattaatg caggcgatcg caggggagcg tgtgaagcga ttcgctggtg
      361 gattaaggac ggtggcagag actgccgtat ccgctcaaat aattgctacg gtcaggtatc
      421 ccgtcgcgac caggagagtg cgctggcatg ttggggtatc gacaggtagc agaatatttt
      481 gctgaaaaat aacgttggtc aacgcggacg gataacacga aatcctgcga actggcaaaa
      541 cctaagtgaa taaaagtaaa aaccccgttt ttggcagcaa gcggggtttt gtgtttatgg
      601 cagtaagcta tgggtggctg ccttgattga ttttagcaaa ctgattaggg agttgcgact
      661 catgattagt caattaccaa actggaagtt tttgctggtc tggagcatcc cttttttatg
      721 gggggtatcc cagttaattg tggcaattaa ggggtagcta tgtcagacaa actcataacg
      781 ctggcgaaga tcctctgtgt aatcgtcggc atttcatttt cactaatgct ggttgctatt
      841 tttcttttcc tgccctggat gatgttgtct tcgtcgggga tgctggggta acagtgactg
      901 atgacatcag cagagcgctg gctttgctat taagtgggtg gcggttggta tcgctgtgtc
      961 tccgatactg tatgggctgg caaaactggt cattgcgctg aaatcgtgaa ctttatttaa
     1021 acagatgagt aatgaaattg ctgtactggt ccgggcaatt cgctggtggt gatatgaacc
     1081 gtattctgta tgtggtgatt attgtactgc tggtggcctg tggtgcgctt agtctggggc
     1141 tgaatcatta ccgcgataac gccatcacct acaaagcgca gcgcgataaa aaagccagag
     1201 agctggagct agcaaacgca accattactg acatgcagca gcgccagcgt gatgttgctg
     1261 cgcttgatgc cagatactca agggaattag ccgatgcgag agctgaaaat gaaactctgc
     1321 gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa cgccacctgc tccggtaccg
     1381 tgcgtgaagc ccccaccacc tccggcgtgg ataatgcaac cgccccccga ctggcagaca
     1441 cagttgaacg ggattatttc actctcagag agcggttgat gctgatgcag aagcagctgg
     1501 aaggggcaca ggactatatc cgcactcagt gctgaactaa gttttgctga tgcgccgtat
     1561 cgtcgccgta ttcccgcata aacagagacc gcagccacct tatctgcgtg agtgtgcggg
     1621 ggtaatcaaa aacgatgcac accgggtttt ctcatttttc acgagatggg agcgatttcc
     1681 cgcgaagccg cctgtccggt gcggtggtgg aagaaaccgg ataaaacaac ctcattgtac
     1741 aaatatcgat caagtatggc gctgctgtgt gaattctgaa aaatcacagc ggtcattatg
     1801 cgtcagtttt taacagagga cgtcagaaag tgacatggca aagctggact ggaaaaagct
     1861 ggagcaggca ttccgacgcg aacatgccga aacgggaata acattactgg actggtgccg
     1921 gaagaaaaag attaattaca acaccgccag aacccgtata aaaatgggca aaatcgatca
     1981 tgaaattgat cataaaaccg atcatgaaat cgatcatgac atctcagatg aagaacccag
     2041 caatgacgcg tgttccgacg atgaaaaatg cgcaaaaaac tctgaaaaaa actgcgcaaa
     2101 ttcggcagaa acgaaacgga ttcgtggttc ccgactttta cctccttcaa acgctttttc
     2161 tcagcgaaac acccacgccg taagacaccg tggatatgcg aagtatcttg aggcagataa
     2221 cctcatggat gatgcgtccg acatggtgct gttcgatgaa ctggtgttca cccgggcccg
     2281 cgcactttca gtaactaagg cacttaaagg gatgttcgcc gacctggaag aggcaactga
     2341 cgtggaaacc cgcgttgctc tttacgacaa aatactcaaa gctgaacagg cccttgaccg
     2401 gaatattgcc cgtatcgagt caattgaacg ctcattgctg acgctggacg tcctggctga
     2461 gacagcacca aaacttcgtg ctgaccggga aagaatcaac gccgccagag ataaactcag
     2521 agcggaaacc gatattctga ccagccagcg ccggggtgtt attacgccag tcagtgacat
     2581 cgtgtcatcg ctgcatgaaa tgagtaattc ggggagactg gatgacattc cggaagaata
     2641 aaacgcgatg tgatgaacct gcagaaatga ctgagaccga acaacgtctg ttcattatga
     2701 caaaactgag caatccctgg tggcgcctca atcatctcta caaaatacag aacgaaaaag
     2761 gtgaactggt caccttcaga atgcgaccgg cgcaacgtca gttgtttcgt aacatgcata
     2821 ataaaaacat tatcctgaaa gcgcgccagc tgggattttc cacagccatt gatatttatc
     2881 ttctcgacca ggcattattc attccgcatc tcaaatgcgg gatcgtcgct caggataaac
     2941 aggctgccag tgaaattttc cgcacaaaaa ttgctgtacc gtttgatcat ctccctgact
     3001 ggctgagagc ctcattcacc atcgttgaac gtcgtagcgg tgcctgcggt ggctatatcc
     3061 tgtttggtca cggctcgagt attcaggtgg caacctcatt tcgctcaggt acggtgcagc
     3121 gcctgcatat ctcagagcac ggcaaaattt gcgcgaaata tccggctaag gcgaaagagc
     3181 tgcgaaccgg tacgcttaat gccgtctctg atgaatgcat tatttttgat gaatccacgg
     3241 ctgaaggcgt gggtggtgat ttttacgaga tgagtaaccg tgcacaggag agcactgcat
     3301 caggcttatt gctgacggca caggattata aattccattt ttacgcatgg tggcaggatc
     3361 ctaaatacag cgccagagtg cctgaaagcg ggctgaagct gtcacgggaa aaaatgacgt
     3421 atttttctgc ggttgagaag gcaatgaaca tcacgcttac cgatgagcag aagcagtggt
     3481 acatcagtaa ggaaactgaa cagcgtgagg aaatgaagca ggagtttccc tcaacgccac
     3541 aggaggcgtt tctgacgtcc ggacgacgtg tgttcagtgc cgaaagtaca ttgcaggcag
     3601 aatcattctg ttcgccaccg atgattgttt atgacattga acctgttaca ggagcgaaga
     3661 ctaaagctca gtctctgcgt gaaggaaata aaaacgagtt gcagcggacg ctgatgaatt
     3721 atctgctggt atgggaactg ccggatccgg atgaagagta tgtttgtggg gcagatactg
     3781 ccgaagggct ggagcacgga gaccgctcat cgctggatgt tgtcaaacgc agtaatggcg
     3841 agcaggtggc tcactggttc gggcatctcg atgctgaact ttttgctcat ctcatttcgc
     3901 aggtctgtcg tatgtataac aacgcgtttg tggggccgga gcgtaataat cacggacatg
     3961 cagttatcct gaaactccgg gaactctatc cgacacgtta tatctacaac gaacagcatc
     4021 ttgaccaggc atatgacgac gatacgcccc gccttggctg gctgacaacc cgtcagagca
     4081 aacctgttct gaccgaagga atgaaaacgc tcctgaataa tggaatatca gggatccgct
     4141 ggtcaggcac attatcggaa atgaacacct acgtttatga cgcgaaaggc tccatgaatg
     4201 cacaggaagg ctgctttgat gatcagctca tgagctacat gattgcccag gagatgcgcg
     4261 ccagaatgcc ggtgagggta aaacagaaaa cggataaacg cagaaccaca cactggatgg
     4321 cacactgatg aaaaatgaaa ttaacaccac agcgatgaaa aacgatcatg gatccacgcc
     4381 gcgtttttct cagcgtcagt tactgtctct ctgttctgat attgacagtc agcctctctg
     4441 gcgtgatgcc gcaaacaagg cctgtgcgta ttatgatggc gaccagctgg caccggaagt
     4501 tatccaggta ctgaaagatc gcggtcagcc catgaccatc cataacctca ttgcccccac
     4561 ggtagatggt gtactgggaa tggaggcaaa aacaagaacg gacctgatag tgatgtcaga
     4621 cgatccgaac gatgaaacag agaaactggc agaggccatt aatgcggagt ttgctgatgc
     4681 gtgccgtctt ggcaacatga ataaggcccg ctccgatgcc tatgcggaac aaatcaaggc
     4741 ggggctcagt tgggtggagg tcaggcgcaa cagcgatccg ttcggacctg aattcagggt
     4801 atccacagtc agcagaaatg aggttttctg ggactggctc agccgggaat ccgacctgag
     4861 tgactgccgg tggctgatgc gtcgccgctg gatggatacc gatgaggcaa aagccacgtt
     4921 cccgggaatg gctcaggtta tcgattatgc tattgatgac tggcgtggtt ttgtcgatac
     4981 cacgattact gaaggccagc ccagtccgtt gatgagtgca tgggaagagt atcagtcatg
     5041 ggatcgcagg gagaatgaat ggcttcagcg tgaacgccgt cgtgtgctgc ttcaggtggt
     5101 ttattaccgt acattcgagc gtcttccggt gattgaactc agtaatggac gggtggtggc
     5161 ttttgataaa aataatctga tgcaggcggt ggctgtggca tccgggcggg ttcaggtgaa
     5221 agtcgggcgg gtaagtcgta ttcgtgaagc ctggtttgtg ggcccacact ttattgtgga
     5281 tcgcccctgt agtgctccgc aggggatgtt tccgctggtt cctttctggg gataccgaaa
     5341 ggataaaacc ggggagccat acgggttaat ttcccgcgcc attccggcac aggatgaggt
     5401 gaattttcgt cgtatcaagc tgacctggct gcttcaggcc aaacgcgtga ttatggatga
     5461 ggatgccacc cagttgtcag acagcgacct gatggagcag atcgaacgtc cagatggcat
     5521 tattaaactg aatccggccc gaaaaaatca gaaaagtgtc gcagatgttt ttcgggttga
     5581 gcaggatttt caggttgcca gccagcagtt tcaggtcatg caggaatcgg aaaaacttat
     5641 ccaggatacc atgggagtgt attccgcatt tctcgggcag gattcaggtg cgacgtcagg
     5701 cgtggctatc agtaacctgg tggagcaggg ggccacaacc cttgcggaaa tcaacgataa
     5761 ctaccagttt gcctgccagc aggtgggaag actgttgctg gcttatcttc tcgatgacct
     5821 gaaaaaacgc cgtaatcatg cagtggtgat taatcgcgat gatcgccagc gtcgccagac
     5881 cattgtcctc aatgctgaag gtgataatgg tgaactgacc aatgatattt caaggttaaa
     5941 tacacatatt gcgctggcgc ctgttcagca gacaccggcg tttaaggcac agcttgcaca
     6001 gagaatgtca gaggttattc aggggctgcc gcctcaggtg caggctgttg tgctcgacct
     6061 gtgggttaat cttctggatg tgccgcagaa acaggagttt gttgagcgta ttcgtgctgc
     6121 gctggggacg ccaaaatcac cggatgaaat gacgccggaa gaacaggaag tagcggcaca
     6181 acaacaggca cttcagcaac aacaggcaga actccagatg cgcgagatgg ctggcagagt
     6241 ggcaaaactg gaagctgacg ccgccagggc acatgcagct gcacaacggg ataatgccag
     6301 tgcacagcgg gaagtcgccc tgacacaggg gcagcgttat gtggatgcgc ttaaccaggc
     6361 acatacggca gaaatcatta ccggcgtaca gaatatggaa caggagcagg acgttcttca
     6421 gcaacagatg ctgtatacgt tacaacagcg gatgaatgaa atgtcgctct gaaaactctg
     6481 gcttcaactg aaccccgtca tcgtacgggg ttttttgttt ccggaggtaa gcgttccggg
     6541 agcggtgcgc ttattcgcgg gggcagcgat aagccttatt tactcaacca ttcggatctg
     6601 tccgataaac agaccatgcg gagttattta tggattttga atttacgggt gaagaaaccc
     6661 cggaacaact ggaaaaaatg ctggaaggac ttggggatgt ggatattgac agtcacgcac
     6721 aggacgtcgt gacggaagat accacggaaa aacatgcgga tgaggaagca cagactcaga
     6781 cgggcgataa caatgtggca ccgacgccgg atgccagtgt ggagcagacg caggacgtga
     6841 aggagccgga agcgaagggg gtgctcaccc gcgacggtaa acacgtcatt ccctatgaag
     6901 tccttgaggc tgaacgttcc ggtaagcaac gggccgaaca ggaagccgca cttcttcgtg
     6961 ggcagatagc tgaagaaaaa cgcagggtgg aactgctgac atctcagatc caccaggccg
     7021 gtatgaagcc cacaccgtta ccggaaaacg aaaaaatttc tgatgagcag attgcccgta
     7081 tcagggagat gtatccggaa attggtgacg cggtggcttc gctcatccgt aaaaataact
     7141 atctccagtc ccgtgttcag caatcaacac agcaggcaga aggtaatggt ggtgagtatt
     7201 tatcaccggt tcttgatgcg atgaatgccg tgccggtgct gaaaacgtgg caggagtccg
     7261 atccggatcg cttctcggtt gctgtatcca tcgacgggaa gctccagaat gaccccgcat
     7321 ggaaagacaa aacgctcact gaacgtttcg ctgaagtggc ccgtcgtacg caggttgctt
     7381 tcggtgaagt cagtgagtcg tctgctgaca accaggcaga caaaacggat atccggaaaa
     7441 cggcggaaga aaaagtgaag accgctgaac aggagcaggc agtacctgct tccccgtcag
     7501 atttaggcac cacggcctcc gtcggaaccg gtgataattt tgaacggtta cttggtgctt
     7561 ctcattcaga ggctgaggcg attatgcgtg gtatgacgaa tgctgaaata gacgcacttc
     7621 tggagaagct cgggtaactt actgaaggag tactgaagta atgacgactg taacatcagc
     7681 ccaggcgaat aagctgtatc aggtggcgct ttttaccgct gccaaccgca accgctcgat
     7741 ggttaatatc cttactgaac agcaggaagc gccaaaagcg gtttcgccgg acaagaaaag
     7801 cacgaagcag accagcgcag gtgcgccggt tgtccgtatc acagacctta acaaacaggc
     7861 cggtgatgaa gtgaccttca gcatcatgca caaactctca aaacgtccga cgatgggaga
     7921 tgagcgtgtt gaaggtcgtg gtgaggatct cagccatgct gacttctccc tgaaaatcaa
     7981 tcagggacgt cacctggtgg atgcaggcgg acgtatgagt cagcagcgca cgaagtttaa
     8041 cctggcatcc tctgccagaa cgcttctggg gacgtacttt aatgacctgc aggaccagtg
     8101 tgcgatagtg catcttgcgg gagctcgtgg tgattttgtt gctgacgaca ccattctgcc
     8161 gacagcggag caccctgaat tcaaaaaaat catgatcaac gatgtactgc ctccgacaca
     8221 tgaccgtcac ttttttggcg gtgatgcgac aagctttgag cagattgaag cggcagatat
     8281 tttttctatt ggcctggtgg acaatctctc cctgttcatt gacgaaatgg cgcatccgtt
     8341 acagccggtt cgtctgtccg gtgatgaact tcacggagaa gatccatatt acgtcctgta
     8401 cgtcacgccg cgtcagtgga atgactggta cacctcgacg tccggtaagg actggaacca
     8461 gatgatggtt cgtgccgtga accgtgcaaa aggttttaat catccgctgt tcaaaggtga
     8521 atgtgcgatg tggcgcaata tcctggttcg taagtatgcg ggtatgccga tccgtttcta
     8581 tcaggggtca aaggttctgg tatcagagaa taacctgacg gcaaccacga aagaggtcgc
     8641 tgctgcaacc aatattgacc gcgccatgtt actgggggct caggcgctgg caaatgctta
     8701 cggtcagaag gcgggcggtc acttcaacat ggttgagaag aaaacggata tggataaccg
     8761 tactgagata gcaatcagct ggatcaacgg tctgaaaaaa atccgtttcc ccgagaagag
     8821 cggcaagatg caggatcacg gcgtgattgc cgttgataca gcagtgaagc tctgattttt
     8881 tcctttcccc atgccgggtt ttcgcccggc tttttcagga gtcattaatt atggcaaaga
     8941 ctatccttgc cccgtcactg agtgaacggg tctatacggg tacgcacggt aatgagtcgg
     9001 tggcagaagg cgtatttacg gtgaatgctg cggaagcgga cagtgttatt catcttctct
     9061 cactgccagt gggcatccgt atcaactcac tccagctggt ttcaacgggt ggtctgggta
     9121 ctgcaaccgt cagcattaag tccggtgagc atgctctcat cgataacagc gaagctgttt
     9181 ctgcaaaatt tgccagatat gtgccagtgg agccgtacac cacacagcgt gacggggagc
     9241 tggttactgt caccattaag actgccgctg caaccggcac cctgaatgtt ctgctgcgtt
     9301 ataccgtggt gggatactga ttaaaacctt ccggcccgcg tcatgcgggc tttttatccg
     9361 gggaattata tgagtgagaa aattgccgtt gtctatatcg gcccaaaacc cgtgaaaaag
     9421 gacaccatta ccggaagccg cacattgttc ccgcgtcttg agccggtgca tgttgacagt
     9481 gcgatggcct ggcaactgct ggggtttccg gatgtctggg ttcgtcatga agagcttgat
     9541 gatgttctga aaaagcaaca acagaatgag cagttgcggc aggcacagca ggcgcaggaa
     9601 agagtgcttg ctgcgcgggc agaagcggag aacagttttg ttgtttctgt taacgggcag
     9661 gaggtggatt taagtaagct cacctcagca cggctggcga cgctgtgtga ggcagaagag
     9721 ctggatattc acaaagaccc gaaagaaacg gctgaggcat tccgtatccg ggtgcgtgag
     9781 gcatttcgcc gtcgtgttgc ggagactgaa cagcatggcg gaactgagtg attttttacc
     9841 gtatgtccgt cgtcagatca gcggtccact gaacattatg atgacggatg ctctgtcaat
     9901 ggctgccgtg gcattcagcc gtcagtcgct ggtgtgccgt cgggaggtta ctgttgtacc
     9961 agtagcagga aaagaaatcg tgcttccgta tgacaaagat gatgaggagt gcgttcatat
    10021 catccgtatc tctgacgata atcatgagct ttttgtcggt cgggatgtgg atatcagctc
    10081 cggacgctcc ctgcgatttg cctgttctcc cggtgaggtg agcgtgcttt atgccgtcgc
    10141 tccgaaagcc ggacgcagcc agataccgga tgaactcctc acatggcctg aagaagtggc
    10201 agcgggggca cttgagcggt tgttcatgca gactggtgtt tcatggtcag acccgttacg
    10261 cgcacagtat ttttctgtgc agttttctga ggggatccgt cgggcatatc gtcatacact
    10321 ggcgacaagt ccgtactcct cataccgcaa tcctgtccgc agacaaaggt tttactgatg
    10381 acgacgatta ctgaaatcat cggacgagtg aacacgcaac tggttgaccc gatgatggtg
    10441 cgctggcctc tggctgagct gtgtgattat tacaatgatg ctgtgcgggc ggtgattctg
    10501 gcaaggccgg atgcaggcgc aagcctggaa acgctcagtt gtgtccctgg cgcacgccag
    10561 actttacctg atggcgcaat acagcttctt gacgtgatat gcctcagcga tggcagtgcg
    10621 attaaaccag tatcccggga ggtgcttgat gcacagtatc ccgactggca catgctgagg
    10681 ggaaaaccgg aatgctttat cagcaacgac ctgtccccgc gcgtattctg gctgtttcct
    10741 gtccctgaca aagagataag tattgatgca gtggtaagcc ggataccgga ggcagtgtat
    10801 gttctgacgc aggacgatga tacgccagtt ccactggaag aggcttatgt taacccactg
    10861 gtggactgga tgttgtttcg tgctttcagt aaggatgctg ccggtggtgc agaatcgggg
    10921 ctggctgcgc agcattatca gagttttgtt gagcaacttg ggatcaaaca gggggcagac
    10981 agtgcattgt ctgcccgtaa aaaagtgttt aacggaggtg gagtgtgagt gttgttgttt
    11041 cggggacgct gaaatctcct gatggtgagg cgatatcagg agcaaatatt accctgacgg
    11101 cgctgacagt ttcaccggat gcgctcagcg gcaccagtgc gtcggcagtg acccgtgaag
    11161 gtggatatta cggaatgacg atggatccgg gggagtatgc ggtttcggtg acggtgaaag
    11221 ggaagactgc tgtctacgga cgtgtgcgta ttgaggggac cgaaagtacg gtgacgctca
    11281 atatgctgtt acgccgcagt cttgttgagg tgagcatacc cggagagctg ctgacagatt
    11341 tccggcagat acagaataat gtggctgatg accttgccac tattcgtcgc ctgaatgaag
    11401 acacggcgac aaaaaacact caggccacac agtcaaaaga aagtgcagca gccagtgcga
    11461 agagtgcatc tgacagtgca aagacggcaa ccagcagggc ggctgaagcc ggacaaaaag
    11521 cgactgatgc cactgaggcc gcgacccgtg caatcacagc agcggggaat gcagaggaaa
    11581 gctcgacccg tgccggagag tctgaaaaag ccgccggagc tgatgcagaa aaagccagac
    11641 agcatgctga aaaggccagg ctggcgcagg agagcgccgg agagatcctt aagcgggcag
    11701 aggctgccac tgtcagtgct gaagaggcca gacgtatggc tgagaatgca cgggggcccc
    11761 gggggcctca gggagaaact ggtccgaagg gggatgtcgg tcctaaaggc gaaacaggtc
    11821 cagtgggccc tcaagggccc gcagggccga aaggtgagcg tggtgacgtt ggtgctcagg
    11881 gggctgtagg gcccgctggt ccgcgtggtg agaa
//
LOCUS       DAZGCQ010000138        11906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000138 DAZGCQ010000000
VERSION     DAZGCQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.138"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..680
                     /gene="lpfA2"
                     /locus_tag="ACHK07_004406"
     CDS             78..680
                     /gene="lpfA2"
                     /locus_tag="ACHK07_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO8104658.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            789..1469
                     /locus_tag="ACHK07_004407"
     CDS             789..1469
                     /locus_tag="ACHK07_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8104659.1"
                     /translation="MYLLRLFFAVTLITQMSMAMAGIVVGGTRVIFYGDKPDTTISIF
                     NKETNLPYLLQTWVDPFNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRE
                     SVFWLNVKNIPPSIDKKNTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNN
                     LIVENPNPIHINVMSVTVDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAI
                     SQPLKKNL"
     gene            1491..4025
                     /locus_tag="ACHK07_004408"
     CDS             1491..4025
                     /locus_tag="ACHK07_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8104660.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSSNYHDDNNDNFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLIQMDAICR"
     gene            4037..5128
                     /locus_tag="ACHK07_004409"
     CDS             4037..5128
                     /locus_tag="ACHK07_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104661.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSGEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGTKPAPDNGFTATATLVIEFTN"
     gene            5156..6238
                     /locus_tag="ACHK07_004410"
     CDS             5156..6238
                     /locus_tag="ACHK07_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104662.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGDERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIIGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6450..7490
                     /gene="pstS"
                     /locus_tag="ACHK07_004411"
     CDS             6450..7490
                     /gene="pstS"
                     /locus_tag="ACHK07_004411"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO8104663.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7577..8536
                     /gene="pstC"
                     /locus_tag="ACHK07_004412"
     CDS             7577..8536
                     /gene="pstC"
                     /locus_tag="ACHK07_004412"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO8104664.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8536..9426
                     /gene="pstA"
                     /locus_tag="ACHK07_004413"
     CDS             8536..9426
                     /gene="pstA"
                     /locus_tag="ACHK07_004413"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO8104665.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9597..10370
                     /gene="pstB"
                     /locus_tag="ACHK07_004414"
     CDS             9597..10370
                     /gene="pstB"
                     /locus_tag="ACHK07_004414"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO8104666.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10385..11110
                     /gene="phoU"
                     /locus_tag="ACHK07_004415"
     CDS             10385..11110
                     /gene="phoU"
                     /locus_tag="ACHK07_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO8104667.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11535..11658
                     /locus_tag="ACHK07_004416"
                     /pseudo
     CDS             11535..11658
                     /locus_tag="ACHK07_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11649..>11906
                     /locus_tag="ACHK07_004417"
     CDS             11649..>11906
                     /locus_tag="ACHK07_004417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO8104668.1"
                     /translation="MSVDGVGKYSVTHKDITNILAGDSQSFLKRSLWEFIRDLFPMYH
                     IKEAKQAIINFVTEPGDKVEMFNRIKSLANEDQQWRFNATTE"
BASE COUNT         3139 a         2732 c         2931 g         3104 t
ORIGIN      
        1 aaataccgat caggtttatc caaatttaat aaatggataa cacacaggaa taatatcaaa
       61 ttaatggagt tatatttatg aaaccaaata tgattgtagg agcattagcg ttaacttctg
      121 tgtttatggc aggtcaccta caggcggctg atggaacagt ccatttccgt ggtgaaatta
      181 ttgacagtac ttgcgaagtc actcctgaaa ctaaagatca ggtcgttgac ttaggcaaag
      241 taaaccgtac agcctttagt ggcgtcgatg atgtggctgc cccgacggct ttttctatcg
      301 atctgactca atgcccggaa acctttaagt ccgccgcaat tcgtttcgat ggtaatgaag
      361 atgctcatgg taatggcaac ctggcaatcg gtaccccgct tgataactct aacgatgctg
      421 ccgctggtat tagcccgagt gataacagtg gggattatac tggtgcgggt gccgttagtg
      481 cagcgaaagg cgtagctatt cgtttatata accgtgcaga taacactcag gtcaagttat
      541 atgaaaattc tgcatcaact ccgatttcta atggtaatgc atccatgaag ttcatggctc
      601 gttatattgc tacggaaacg actattgacc ctggtacagc taacgccgac tcgcagttta
      661 cagttgaata tataaaataa atcattctta acataatacc ttaaataaaa tggcgctcgc
      721 cattatggtt ctcttttact taaaagaagg gcgtcattat tatattttta tgagatacat
      781 ttatgcctat gtatttactt agattgtttt ttgctgttac tttgattacg cagatgtcga
      841 tggctatggc tggtattgtt gttgggggaa ctcgcgttat tttttatggt gacaaacctg
      901 atactacaat ctctattttt aataaagaaa cgaaccttcc ttatctattg cagacatggg
      961 ttgatccatt taataaagag gataaaagca aaccgccatt ttctgtgata cctcccgttt
     1021 ctcgcattga accgcagcaa gagaaaatat tacgcatttt aaaaacggct ggcgagttac
     1081 caaaagatcg agagtccgta ttctggttga acgtgaaaaa catccctcct tcaattgata
     1141 aaaaaaatac caatgttctt gagatagcca taaaaacaag aattaagtta ttctggcgac
     1201 cagccgcggt aaagataatc cctgagcagg ctattaaaaa tgttaaatgg tcgcggcagg
     1261 gtaataatct catcgtcgaa aatccaaatc cgatccatat taatgtaatg agtgtgacgg
     1321 tggacgataa agacgtgtcg ctaaatatga tccgcccttt tgaaacattg acattaccaa
     1381 ttccggctgg cgtggcagga aaatcgctcg tttggcgctt tattaatgat tacggtgcaa
     1441 ttagtcaacc actaaagaaa aacctttaag cgccggataa ggatatatcg atggcctctt
     1501 cgcatctttt cattacgctt gcatcgggca tatgtctgct ctgttccata tctgcttttg
     1561 cccgggatag cttgttcaac cccagattac tggaactgga tcatcctgcg gataatattg
     1621 atattcacca gttcaaccgt tcgaatacct tacctgcggg aacatacaaa gttgatgtga
     1681 tgatcaacgg catgctcttc gaacgccagg aagttaaatt tgtccaggat aaccctgatg
     1741 ctgaactcca cccatgctac gtggcgataa aaaacgtact ggcgacctat ggtataaaag
     1801 ttgatgcgat aaaatctctg gcgaatgttg atgacaaaac atgcgtaaat ccagttccgc
     1861 tgatcgacgg ggctacctgg ttactggacg ccagtaaact tgcattgaat attactattc
     1921 cgcaaattta tctcaacaat gcagttaatg gttatatcag cccttcccgt tgggatcagg
     1981 ggatcaatgc catgatgatg aattatgatt tttcggcatc gcataccatc cggtcaaatt
     2041 atgacgacga cgatgacagt tattatctga atttgcgtaa tggtattaat ttaggcgcat
     2101 ggcgttttcg taattacagc accctgaatt cttatgacgg taatgtggac taccattccg
     2161 tcagtaatta cattcagcgc gacatcatgg cattacgtag ccagattatg attggcgata
     2221 cctggacggc aagcgatgta tttgatagta cacaggtgcg tggcgtgcgg ctgtataccg
     2281 atgacgatat gttgccctcc agccagaatg gctttgcgcc agtggtacat gggattgcga
     2341 aaactaacgc cacggtgatc atcaaacaaa acggctacgt tatttatcaa tcagccgtac
     2401 cacagggcgc atttgccctc accgacttaa acacgaccag tagcggcggc gatctcgatg
     2461 tcactatcaa agaagaagat ggcagcgagc agcactttat tcagccattt acttcactgg
     2521 ccattctcaa gcgtgaaggt cagaccgatg tagaccttag cattggagaa gtgcgcgacg
     2581 aaagcggctt tacgcctgag gtcttgcagt tacaagcaat gcacggtttc cctttgggaa
     2641 taaccttgta tggcggaaca caactggcaa atgattacgc ttctgccgcg ctgggtattg
     2701 gtaaagatat gggggcgctg ggcgcgattt cttttgacgt gactcatgcc cgctcgcagt
     2761 ttgactacgg cgataatgag agtggtcaat cgtatcgttt tctctattcc aaacgttttg
     2821 aagacaccaa taccaccttt cgtctggtgg gttatcgcta ctctacggag gggttctaca
     2881 ccctcaatga atgggtgtcg cgacaggata acgattctga tttctgggta acgggcaacc
     2941 gtcgcagccg cttcgaaggc acctggacgc aatctttcac gccaggctgg ggcaatattt
     3001 atttaacatt cagtcgacag gaatactggc agaccgatga ggtcgagcgt ttattacagt
     3061 tcggctataa caacaactgg cgaaacatct cctggaacgt ttcctggaac tatacggact
     3121 cgatcaagcg ctcatcgagc aactatcatg atgataacaa tgataatttc ggcaaagaac
     3181 agattttcat gttctcaatg tcgataccgc tatcgggctg gatggaagac agctacgtca
     3241 actattcgtt aacgcaaaac aaccaccatg aaagcacgat gcaggtcggt ctgaacggaa
     3301 cgatgctgga agggcgtaac ctgtcttata acgtacagga atcgtggatg cactctcctg
     3361 atgactccta cagcggcaat gccggaatga cctatgacgg gacttatggc tcggtcaatg
     3421 gcagctattc ctggagccgt gactcccaac attttgatta tggcgccaga ggcggcgtgc
     3481 tggtgcatag tgacggagtg accttctcgc aggaactggg cgaaacggtg gcattagtca
     3541 aagcgccggg cgcagaaggc ctgtccattg aaaacgccac cgggatttct accgactggc
     3601 gtggttatac cgtaaaaacg cagcttagcc catatgacga aaaccgcgtg gcattgaaca
     3661 gcgactattt ctccaaagcc aatattgaac tggaaaacac cgtcatcaac ctggtaccaa
     3721 cgcgcggtgc ggtggtgaaa gccgaatttg tcacccatgt cggttatcgc gtgctattta
     3781 acgtccgcca ggtcaacggt aaaccaataa tgtttggcgc gatggcaacc gcctctctcg
     3841 aaacgggcac agtcaccggg attgtcggtg ataacggcga actgtatctc tccgggatgc
     3901 ctgaaaaagg cgagttttta ttgagttggg gacaagctgc ggatgaaaaa tgtaaggcgg
     3961 cctatcacat cacccataaa cctgatgata ccagcctgat tcaaatggat gcgatttgtc
     4021 gctaaaggaa gcatgaatga aaaaaatatt atcagggttg attctgctgc tttgctgtcc
     4081 ttatggtttc gccgctaatg gtgatggcgt ttgttttaat agtgatggcg caacgcacat
     4141 gtcaaattta tcatttggtc cgctgacggt ggctgcggcg aataaccact ccggatacaa
     4201 tattttcgag gcactgagca acacgactgg aacatacccg gtgcgctgtc actgtgatga
     4261 cacgcatggc ggaccgggcc aacaaacagc attttttcct atcttctaca cgggggatgc
     4321 cgcaccgggg cttgtgcttg agcgcactct taatgggtta aattactatg ctctgaatga
     4381 ttatttatcg gtcggcgtga cgatttttat tattaataac caatatgcgg ccattccttt
     4441 tgaacactta tccaaccaat ccacctcacc gcaacatacc tgtggagcag gtaataatgg
     4501 gagcactgta aatctggatt cagggcgctc ggcaaaatta tctttctatg ttcggcattc
     4561 tattactggc acggtgacaa tacccacaac ggaagtcgcc tggttatacg cgggcatgtc
     4621 cgatcatttt cccaaaacga cccccgtttc taaagtgaca attcgcggac aattaacggc
     4681 cccgcagaac tgtgagttaa cgccaaatca gagcatcgat gtcgattttc aaaaaattaa
     4741 tagcggtgag ttctcctcaa cggcgggttc aattattgca gaacgaaaga ttaaaaccga
     4801 agtcacagta tcctgtaccg ggatggaaga cgtaaggtct acggaggtgg tgagtgcgtc
     4861 gatgattgcg gcaaacagaa gtgccgatgc caccatgatc gtgacgagta atccggatgt
     4921 gggaattaag atttttgata agaacgaccg tccagtgaat gtggatgggg gcaacttacc
     4981 cgctgatatg ggtgctatta gtcgattagg aaaaaccgat ggtagcgtaa cgttttattc
     5041 agcgcccgcc agtctgacgg gcacaaaacc agcgcctgat aatggattta ccgccacagc
     5101 cacgctggtt attgaattta ctaactaatc ttgtgttagt aaaaggaata atttcatgaa
     5161 aaaatggcat tatatatttt gcataattct ctttcattta gggttgccgt gcgggtatgc
     5221 ggcaaatgat ggcacgtgtg caacaagagg cggcacacat acattaagcc ttaattttcc
     5281 tctgacaacg gtcagtgcag caaacaatgt gcctggaaat acattaatag atattgctaa
     5341 tgcaacatct tctgaaaatt atagcgttct gtgtaactgt gattcaaaac atagcaatgg
     5401 cgcttatcac gaaatatatt ataccgcaga ccctgctccc ggtatggttt atagcaccac
     5461 cgcaagtggt cttgcttttt actatcttaa cgaatatgtc gatgtgggaa caaaaatatc
     5521 tgtgctaaat gcggggtata cggcagttcc ttttgaacat gtttccaacc aggcaactac
     5581 aacagatcac acttgtcagg gaaacaaaac tacagcggtt ggcgtgagcc tgaaaactgg
     5641 agcagatgcg aagatttcat ttcgtattaa acgttcaata aatggaacgg tagtaatacc
     5701 tatcaccgat attgcattgc tgtatgccaa catatccagc accacgaccc gtggtgaggc
     5761 gattgcaaaa gttcgaattt caggcagttt gaccgcacca cagtcttgtc agataaatgc
     5821 aggacaggtg atttattttg attttgatac tattcctgcg tccgaatttt catctaccgc
     5881 cgggcaagcc attacttcac gaaaaatcac taaaacagtg agtattgagt gtacggggat
     5941 gggggatgag cgtacgcaga aagtcgatgc ttcttttacg gggacgaacc gaagcagtga
     6001 cgatacgatg gtggcgacag acaatgctga tgtcgggatc aaaatttaca ataaatcgaa
     6061 tgctgaagtt agcgtcaaca acggcaagtt acccgcagac atgggcaaca cgaccattat
     6121 tggtcgtaaa aatggttcgg taactttttc ggcagcacct gccagcttta ccggtgcccg
     6181 gcctcagccc ggcgttttta acgctaccgc gaccttaacc attgaatttg taaactaacc
     6241 ttttgcggct gttttatgac agccgttcat atttcctctc ctttcatgaa aaaatgctga
     6301 tatttttccc cttcaatatc aataagtaat aactttcctt tgggtgtcat caaactgtca
     6361 cattccttac atataactgt cacctgtttg tcctattttg cttctcgtag ccaacaaaca
     6421 atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc gtcgcaactg
     6481 ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt tgcagaagca agcctgacag
     6541 gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact taccagaaag
     6601 aaaccggtaa taaagttaat taccagggta tcggttcttc cggtggcgta aaacagatta
     6661 tcgccaatac cgttgatttt ggtgcttctg acgcgccgct gtctgacgaa aaactggctc
     6721 aggaaggtct gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc
     6781 ctgggttgaa gtctggcgag ctggtgctgg atggtaaaac cctcggcgac atctacctcg
     6841 gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact gaatccgggt ctgaaactgc
     6901 cttcacagaa cattgctgta gtacgccgcg cagatggctc cgggacttcc ttcgtcttca
     6961 ccagctacct ggcgaaagtg aatgaagagt ggaaaaacaa cgttggtact ggctctaccg
     7021 taaaatggcc gatcggtctg ggtggtaaag gtaacgacgg tatcgctgcg ttcgttcagc
     7081 gtctgccggg tgcaattggt tacgttgaat atgcttatgc gaagcaaaac aacctggcgt
     7141 acaccaaact gatctccgct gatggtaaac ctgttttgcc gactgaagaa aacttcgcta
     7201 atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca ggatctgacc aaccagaaag
     7261 gcgaagatgc gtggcctatt acctctacca cgttcattct gatccacaaa gatcagaaga
     7321 aaccagaaca aggcacagaa gtgctgaaat tcttcgactg ggcgtacaaa accggggcta
     7381 aacaggcgaa cgacctggat tacgccagcc tgccggatag cgtagttgaa caggttcgcg
     7441 ctgcgtggaa gaccaatatt aaagacagta gcggcaagcc gctgtactaa taaaactcca
     7501 ggccggataa ggcattaaac ggcttatccg gcattacaaa atgactttgt aaacgcgttt
     7561 aactgaagag taacttatgg ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg
     7621 cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat tgatgttggg
     7681 tggcattatt gtctctctga tcatctcctc atggccgagc attcagaaat ttggtctggc
     7741 tttcctgtgg accaaagagt gggatgcgcc gaacgatatc tacggggcgc tggtgccgat
     7801 ctacggtacg ttggtgactt cgtttatcgc gctgctgatc gccgtcccgg tgagtttcgg
     7861 tatcgccctg ttcctgactg agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc
     7921 cattgagctg ctggcagcca ttccaagtat cgtttacggc atgtggggcc tgtttatctt
     7981 tgcgccgctg ttcgccgttt acttccagga gccggtcggc aatatcatgt cgaatatccc
     8041 gattgtcggc gcgctgttct ctggcccggc atttggtatc ggtatcctcg cggcaggcgt
     8101 gatcctcgcc atcatgatta ttccgtacat tgcggcggta atgcgcgatg tgttcgaaca
     8161 aaccccggtg atgatgaaag agtcggccta cggtattggc tgcaccacct gggaggttat
     8221 ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc ggcggcatta tgctggggct
     8281 gggccgcgcg cttggtgaaa ccatggcggt gacctttatc atcggtaaca cctaccagct
     8341 cgacagcgcc tcgctgtaca tgccgggcaa cagtatcacc tctgcgctgg cgaacgaatt
     8401 tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt
     8461 tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc tggctaagaa
     8521 tgagggggca cgctaatggc tatggttgaa atgcaaacca ctgcggcgct tgctgaatcc
     8581 cgccgcaaaa tgcaggcgcg tcgccgcctc aagaaccgta ttgcactgac gctctcaatg
     8641 gcgacgatgg ccttcggtct gttctggctg atctggattt taatgtccac catcactcgc
     8701 ggtatcgatg gtatgtcgct ggctctgttc actgaaatga cgccgccgcc caatacggaa
     8761 ggtggtggtc tggcgaacgc tctggcgggt agcgggctgt taattttgtg ggccacggta
     8821 ttcggtacgc cgctgggcat tatggcgggg atttatctgg ctgaatatgg tcgtaaatcc
     8881 tggctggcag aagtgattcg cttcattaac gacattctgc tctctgcgcc gtcgattgtg
     8941 gttggtctgt ttgtttacac catcgtggtg gcgcagatgg agcacttctc cggttgggcg
     9001 ggtgtgattg cgctggcgct gttgcaggta ccgattgtta tccgcaccac cgagaacatg
     9061 ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg cactgggtac gccgaagtgg
     9121 aagatgatct ccgcgattac gctgaaagcg tcggtgtccg gaattatgac cggtatcctg
     9181 ctggcgattg cccgtattgc cggtgaaacc gcgccgctgc tgtttaccgc gctctccaac
     9241 cagttctgga gcacggacat gatgcagccg atcgccaacc tgccggtgac gatctttaag
     9301 tttgcgatga gcccgtttgc ggaatggcag caactggcct gggccggggt attgatcatt
     9361 accctgtgcg tactgctgct gaacattctg gcgcgcgttg ttttcgcgaa gaataaacac
     9421 ggttgatatt gctgacacgg ttttcccctc tccccagagg ggcgagggga ccgaccgagc
     9481 gcctttttga ctctgtacac ggttaacact ttgccggatg cggcgtaaac gcctgatccg
     9541 gcctacggta agcctgatta gcgaagtgca tcaggcacga tgaggaaaag attgcaatga
     9601 gtatggttga aactgccccg agtaaaattc aggttcgtaa tttgaacttc tactacggca
     9661 aattccatgc cctgaaaaac atcaacctgg atatcgctaa aaaccaggta acggcgttta
     9721 tcgggccgtc cggctgcggt aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac
     9781 tgtatccgga acagcgtgcg gaaggtgaaa ttctgcttga tggcgacaac atcctgacca
     9841 actctcagga tatcgccctg ctgcgtgcga aagtgggcat ggtgttccag aagcctacgc
     9901 cgttcccgat gtccatttac gacaacatcg cctttggtgt ccgtctgttt gagaagctct
     9961 cccgtgccga catggacgag cgcgtacagt gggcattgac caaagccgca ttgtggaacg
    10021 aaaccaaaga taaattgcac cagagcggtt actctctctc tggtggtcag caacaacgtc
    10081 tgtgcattgc gcgtggtatc gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt
    10141 cggcgctcga ccctatctct accgggcgta ttgaagagct gatcaccgaa ctgaagcagg
    10201 attacaccgt ggtgatcgtc acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca
    10261 cggcgtttat gtacctgggc gaattgattg agttcagcaa cacggacgat ctgttcacca
    10321 agccagcgaa gaaacaaaca gaagactaca tcaccggtcg ttacggttga ttcaggagtg
    10381 cgttatggac agtctcaatc ttaataaaca tatttccggc cagttcaacg ccgaactgga
    10441 aagtatccgc acgcaggtga tgaccatggg cggcatggtg gagcagcagc tttctgatgc
    10501 aatcaccgcg atgcacaacc aggacagcga tctggcgaag cgcgtcatcg aaggcgacaa
    10561 gaacgtcaac atgatggaag tggcgatcga tgaagcctgc gtgcgcatta ttgccaaacg
    10621 tcagccgaca gcgagcgacc tgcgtctggt tatggtgatc agtaaaacca ttgctgaact
    10681 ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact gcgctggaga aattctccca
    10741 gcagcatcag ccgttgctgg taagtctgga gtcgctgggc cgtcatacca tccagatgct
    10801 gcacgacgtg ctggacgcgt tcgcgcggat ggacattgac gaagcggtac gtatttatcg
    10861 tgaagataaa aaagtcgatc aggagtatga aggtattgtt cgtcaactga tgacctacat
    10921 gatggaagat tcgcgtacca ttccgagcgt acttaccgcg ctattctgtg cgcgttctat
    10981 cgaacgtatt ggcgaccgct gccagaatat ttgtgagttt atcttctact acgtgaaggg
    11041 gcaggatttc cgtcacgtcg gtggtgatga gctggataaa ctgctggcgg gaaaagaccc
    11101 caaagagtag tgctaactgt aggccagata aagctttttg ccgcatctgg caatcagccc
    11161 ggtcatacat agttaccggg ctgataaact ttctcactga aatattgcca cacaaactca
    11221 cctcgaaact ctccccagta cattgcatat acattattac atttgaatta ttttactttt
    11281 aatgtagtgc cacgctataa aaacattttt gttattcttt tcatttatat cgctacctgc
    11341 acagagaatg gaaaagaaaa caaaaaacag ggtgaaagcc ctatatcgaa acatacataa
    11401 tgagcgattt tttacagtat tcataggtgg tttagccata atcgtctcaa accaacaccc
    11461 aggtatgctt tttaagacaa atcggctatg tgttgtgctt gggcgaataa atattgtgtt
    11521 caggagaaat gattatggct atatcatcat atagtacagt tacagataaa tttgctaatc
    11581 tgactaaaat accagatgaa aatatgaatg aaaaaaatag aacgcaaaaa tattcaggaa
    11641 aaattggttt gtctgtagat ggtgtgggta agtattcagt aactcataaa gatataacaa
    11701 acattttagc tggtgatagt caatcttttc ttaaacgaag tctttgggaa tttatacgcg
    11761 atctttttcc tatgtatcat attaaggagg ctaaacaagc cattataaat tttgtgacgg
    11821 aacctggtga taaagttgag atgtttaata gaattaaatc tctcgccaat gaagatcaac
    11881 aatggcgttt taatgcgact acagaa
//
LOCUS       DAZGCQ010000139        11543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000139 DAZGCQ010000000
VERSION     DAZGCQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.139"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..452)
                     /locus_tag="ACHK07_004418"
     CDS             complement(<1..452)
                     /locus_tag="ACHK07_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO8104669.1"
                     /translation="MSNILWGDLETFCEIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAIPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRAT"
     gene            479..1078
                     /locus_tag="ACHK07_004419"
     CDS             479..1078
                     /locus_tag="ACHK07_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104670.1"
                     /translation="MDTKDIISLVGVIISAITAPVGVYKLFKDASWFLPKTTKFSHIL
                     KNYPEHIEPSDVEFMKAEIKREVKKSILGISSQKLRTLVIYVRTYSELKMPFWQWGYL
                     APHIQCKYDRFFIRYKGKYKRCRWCSKVVSIFYLADGLIFFCWSLNYGAVFIGMGAVL
                     MLLCIWMAFMFWFLFPGRGVIKKYNSQLLKIDASKYQAK"
     gene            complement(1193..1957)
                     /locus_tag="ACHK07_004420"
     CDS             complement(1193..1957)
                     /locus_tag="ACHK07_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO8104671.1"
                     /translation="MSTQIVFKSHVLETIEHNGKTWFTAATLATALEYSDARSVTNIY
                     TRNTDEFTLGMSEVIKMVTSGNLQASRRIFSLRGAHLVAMFATTPVAKEFRRWVLDLI
                     EKETAVPQSSTILAPHRECLPKMVYHHRSKYNPYRAYAWDGEKSVYVGCYPTVGEAVA
                     AQERFYRNGSTKRIQKAQPAVSDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEKM
                     DSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            complement(2339..2887)
                     /locus_tag="ACHK07_004421"
     CDS             complement(2339..2887)
                     /locus_tag="ACHK07_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO8104672.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLISPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(2903..4204)
                     /locus_tag="ACHK07_004422"
     CDS             complement(2903..4204)
                     /locus_tag="ACHK07_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO8104673.1"
                     /translation="MPDVHARLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGKRAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGNPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(4197..4715)
                     /locus_tag="ACHK07_004423"
     CDS             complement(4197..4715)
                     /locus_tag="ACHK07_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFO8104674.1"
                     /translation="MPEAWKDISGFEGIYQISDAGNLRSLDRTVLNKGSGCTYPIKGK
                     SLKPRYDADGYLITDLWKAGKKVTVKIHRLVAAAFIPGSAPEVDHINGRRDDNRAVNL
                     RWASLSQNRANSHTRKNKSGVVGVRFSEGKKNPWQAYGRLNGKFKSLGHFPTKQLVAA
                     ARRKHIQEVMNA"
     gene            complement(4718..5620)
                     /locus_tag="ACHK07_004424"
     CDS             complement(4718..5620)
                     /locus_tag="ACHK07_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104675.1"
                     /translation="MSLETSLELNNQLLEQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(5617..5766)
                     /locus_tag="ACHK07_004425"
     CDS             complement(5617..5766)
                     /locus_tag="ACHK07_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104676.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(5868..6161)
                     /locus_tag="ACHK07_004426"
     CDS             complement(5868..6161)
                     /locus_tag="ACHK07_004426"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO8104677.1"
                     /translation="MAGKPAGKVKADGYVTICLDGKNQYVHRLVWEYHFGDIPAGMEI
                     DHINHNRADNRIENLRLVTRAENAKNLAQRSPTKRNGGQLERIKKALAGTNHR"
     gene            complement(6349..6555)
                     /locus_tag="ACHK07_004427"
     CDS             complement(6349..6555)
                     /locus_tag="ACHK07_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104678.1"
                     /translation="MNTYELTTKYAPAFEVVENGPDATDYVVATVKGENPARLRAAAY
                     QERNGYLGYGYTIRPLTNSSESSM"
     gene            complement(6940..7569)
                     /locus_tag="ACHK07_004428"
     CDS             complement(6940..7569)
                     /locus_tag="ACHK07_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO8104679.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            7677..7901
                     /locus_tag="ACHK07_004429"
     CDS             7677..7901
                     /locus_tag="ACHK07_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8104680.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            7905..10124
                     /locus_tag="ACHK07_004430"
     CDS             7905..10124
                     /locus_tag="ACHK07_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO8104681.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFARWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSNGCSETSIK
                     YFPKGTGGFELGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILITPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            10431..10712
                     /locus_tag="ACHK07_004431"
     CDS             10431..10712
                     /locus_tag="ACHK07_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104682.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            10781..11203
                     /locus_tag="ACHK07_004432"
     CDS             10781..11203
                     /locus_tag="ACHK07_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO8104683.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            11283..11384
                     /locus_tag="ACHK07_004433"
                     /pseudo
     CDS             11283..11384
                     /locus_tag="ACHK07_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            11373..11449
                     /locus_tag="ACHK07_004434"
     tRNA            11373..11449
                     /locus_tag="ACHK07_004434"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11407..11409,aa:Arg,seq:tct)
BASE COUNT         2801 a         2639 c         2939 g         3164 t
ORIGIN      
        1 ctggtggccc gacgcagttt gctgtttttc gggcgtggct tacagaacag ctggatcagc
       61 gctttacctt ctttgtcctt cgctttgtct tgcgggacgc ctagtacttc gcagagttcc
      121 cccagagacc ccgggagacc gtgcgccaac gcctgcacca tcgtgtcgcg ccaacgttcg
      181 actggcggtg ccagtcgcgg tattgcataa cgcagaacgg tgcggtcgaa gtgagagtta
      241 tggaaataaa gcagggtttc agggtctgcg attgcttcgt ataagccgtg tggaatacca
      301 ccaccggcag tgatatccca cacgtttact ggcccgtcgt tgatagccca tgcgaaaagc
      361 atcacttcaa cgccttcggc atacgcatgg gtgccattat tgataggtat ttcacagaaa
      421 gtttccaggt cgccccatag tatattggac attgtattta ctccgaagaa ttagctacat
      481 ggatacgaaa gatattatct cacttgttgg agtcataatt tcagccataa ctgcaccagt
      541 cggtgtttat aagttattta aagatgctag ttggttttta ccgaaaacaa caaaattctc
      601 gcatattctg aagaattatc cggagcatat tgagccttct gatgtagagt ttatgaaggc
      661 agaaatcaaa agagaagtta aaaaaagtat attaggtata agtagccaga aattaagaac
      721 gttagttatt tatgtcagaa cgtattctga attgaaaatg ccgttttggc agtggggtta
      781 tttagcaccc catattcagt gtaaatacga tagattcttt atacgctaca aaggtaaata
      841 caaacgatgt cgctggtgtt ctaaagttgt ttctatcttt tatttggcag atggcttgat
      901 atttttctgt tggtcgttaa attatggcgc tgtttttata ggaatgggcg ctgttttaat
      961 gttgctgtgt atatggatgg cttttatgtt ttggtttctt tttccgggac gaggcgttat
     1021 aaagaagtat aattcccaat tattgaaaat agatgcaagt aagtatcagg ccaaatgata
     1081 ttacaggtct gatactttgc ttttttaaat cagtgttttc agacaaaacg aagtcctccg
     1141 taaattaaac ggtgataaaa agcccctggc tgagcagggg cgaactgaag gattaacgag
     1201 gtaaagtggg tgggatatat ctgccgatgc ggtcttctat ggtcggcaga atgcacatgc
     1261 tgtcgctgaa gcggtcatgc aactggtaaa ccagtttcga atccattttt tcgagagcag
     1321 gcattaactg cgcccgccag atttcgttga tgcgccggaa gttatgacag acggcgcgga
     1381 gattgttgat gaacatttcc ttctctgcgt cactgactgc tggctgcgct ttctgaatgc
     1441 gtttcgtgct gccattccgg tagaagcgtt cctgagctgc aacagcctca cccacggttg
     1501 gatagcaccc gacgtaaacg cttttctctc cgtcccatgc ataagccctg tacggattgt
     1561 atttgctccg gtgatggtaa accattttcg gcagacactc cctgtgcggc gccagtatcg
     1621 tggaggattg cggaacagcg gtttctttct cgataaggtc aagcacccaa cggcggaatt
     1681 cttttgctac cggagttgtt gcgaacatag ctaccaggtg ggcaccgcgg agggagaaaa
     1741 tgcggcgcga ggcctgtaaa ttccccgagg taaccatttt gattacctcg gacatgccga
     1801 gagtgaactc atccgtgttg cgggtgtaaa tgttagttac gctgcgagca tccgaatatt
     1861 ccagcgcggt agctagggta gccgcagtaa accaagtttt gccgttatgt tcgatcgttt
     1921 cgagaacgtg ggatttgaat acgatttggg tactcatgac gattttcctc ttaactgagt
     1981 taatcgccac catcaggtgc taatcatcgt ggtggcgaac tgtgcggggt tagcactacc
     2041 gggttaagag gaaccggcgc ggatttctcc gcccccacac agcccgccat aatgcgaacg
     2101 tggcaaagct tgcgacaata aaaagacgct ggcgcgtctg gtgtcgcctc ttaaacttcc
     2161 ggggtgctaa tcccgatctc catttgggag attcggtgag cataaaccgg aagggggcga
     2221 tgagtcaata tcggggtagc agggcgactg ggtgctaacc agaacaatac aggtagctcc
     2281 gggcatattc ccctttgcaa agacacccgg cgtttaaccg ggcgtctgtg aagcaggatt
     2341 aaatcagtgc ttcagcatca gcaccttcgc tgatatcgtc gaaatcgtca gcgcttgcta
     2401 ctccgccgcc agcgaatgca tcgccgtctc gcaggaactg gactccgccg agtgaggcat
     2461 taatgcgttt accgaaatta ttgtcctgtg cccagatatc gataacggcg tttacatagc
     2521 accctgcata gggacgtcca tcagcctgaa ttagtggcga acgatcgcga tcaagaacgg
     2581 ccgggcgcgc tttgttagca gcattcagga agaaattacc ggggaagcct tcatactctg
     2641 ctttttcatc accatcatgc aggcacagat tgagtttttt ctccagctgg ttataaatgg
     2701 actcccactt ctccccccat ttttccttcg ctacctgctt catagcttta cggatttctt
     2761 ccagttgtgg gtgtttggga gatattaaaa atactgcgga gaaacgcgga tcgccttcgc
     2821 cgtttacagt tttagcttca aacagagcag gaaaagctaa tctcacgtta tttaatttaa
     2881 tcttcatgaa agtctccttt aatcagatga ggtctgcggc gagcgtatcg tcggatacgt
     2941 cgtcgaaatc atttacaggg ttgatattga gtgcggggcg tgggtccgac tcgggaacga
     3001 cggttggttt accatcagct cgtgttatca gtgcctcgac ttttgaccaa cggcgcggac
     3061 tggccttttt gataagtttt tcggctttgg ttgggctaat cagcttaaga tcgaaaacct
     3121 cctcggtttt ataacggaac tggtctttca gcagcgcgcg ggcggcttct tcatcactcc
     3181 aggcccggtt accttgtttt cctgttacca gtttaaaccc cggtaccgga tttccggcat
     3241 tgagttcatt atgaacccgg tcccgtactg cctttagcca ggattcaata aagtcggcct
     3301 gactatagat ctccgcaagc tgctcaatgg ttaacagagg tacacgtgcg ctggcattgg
     3361 tgatgatttc gctgacaggc tttgtcagat cttcaaaatc gctggccgct gtttgtaaat
     3421 gctgcatttt ctgggcagtg caaatagctt ttgctttaca gaagcggcac tgtttttctc
     3481 caggtatgaa gttttccagc ggtagtgtct caatgccttc gcattcagca atattgagaa
     3541 caaggatcgc actggttgcg gcctcctgtg ctcgtttacc gaaagactga agctcctgta
     3601 cggttaaaga ccattctgaa acgtggttga gccttggctg gtgaataaat aatcttacag
     3661 tctcaaagtc atacagcatg ctgaattgtt cgagagcacc cagagcatac agttgtagtt
     3721 gctcattttg ttctgcatca atgcggacgc ctttgccata tttcaggtcg tggatttgta
     3781 attcgttacc agcgatgatt atgccgtcgg cagttccgaa agattcttcc acacccgtta
     3841 tatgtgagaa atcaacacgt tgttcaacca atagttcatt attctgtgca agagtccaga
     3901 ccgtatcaac ataccggcca acggcttcga ccatttcatc atccacctgt gggccagatg
     3961 tatcatcagg attttcgcga agggggtatg agccgagaaa catagaaaca ttgcatccgg
     4021 cgtagtgttc cgggtggctt tgcctgtttc gtagaacttt ttcagcaagc gcgtgcgctg
     4081 cagtgccctc gattgcaaaa gttgtttctt tatccggttg tgtggcctcc agcgccagac
     4141 tacccgcaca ccttagccaa cgatgtgcag atgatggtga taatcgtgca tgaacatcag
     4201 gcattcatga cctcctgaat gtgttttcgc cgcgcagccg ctacgagttg tttggtgggg
     4261 aaatgaccga gagatttgaa tttgccgttt aatcgtccgt aggcctgcca cgggtttttc
     4321 ttaccttcgc tgaaccgaac gccaaccact ccagatttat tttttctggt gtgagagtta
     4381 gccctgtttt gtgaaagaga ggcccagcga agatttaccg cccggttgtc atctcgacga
     4441 ccgttaatgt ggtcaacttc aggggcagat ccgggaataa atgcagcggc aacgagacgg
     4501 tgtattttga cggttacctt tttacccgct ttccataagt cggtgatgag atatccgtct
     4561 gcgtcatagc gtggttttag tgacttacct ttaatgggat aggtgcagcc agaacctttg
     4621 tttaatacag ttctgtcgag gcttctcagg ttgccagcgt ctgaaatctg gtaaattcct
     4681 tcaaagcctg aaatatcctt ccaggcttcc ggcatgatta accctccagt gctttttcag
     4741 ccagggtgat tacttcagcg agattttcat ctgttacttc accaagtttc ctggctccct
     4801 gtttttccag aattgcaata gcttctgccc ggtaaccccc ttttgctaac tggaggatca
     4861 acccttcagc ttgtttgcgt agtgccgcga aatcaattgt atggtcatct ttggcgtcat
     4921 tattctggct ggaatttgct gcgtctctgc gtgcaaattc ttcctgcagc tgaaggtact
     4981 caacacggtt gatctcgata tggccttttt tcagcatctc gttcaacttg cgtaaggtgt
     5041 ggagttcact ggctgctgtg ccggatacat ttttgacgta aaacggcccc gtgcgttctc
     5101 catctttgtt actggccttt ttcggcttaa cttcatcacg cccatctgca ggtgcatcaa
     5161 gtagctgctc ggcaaaagca cgtcgctcgc cgatggttgg caggtcgtcc cagaacttaa
     5221 gaatgttacg ggacaggtcc aggagagcag gtttaagcag cgccctggct cgtttgacgc
     5281 cctgtaatgc gctgtcgaga gcatcaatct gaactactcg tttatcgcct tcagcatcac
     5341 ggtaggcaac agcacgttgc agcatgtctt ctgtgatagg ggtggctacc gggtagaaac
     5401 cagccagtgc gataacgtcg ctgaactcca gatcatccag tgtcattgcc gctgacatgt
     5461 tttcagcttc agttgctgta tcccgacatt cctgcactcg tgaaatcgtg tcaggatgca
     5521 taacaatacc tgatgccatt gtgcggataa gacgttcaag cagcgcatta tgttgttcca
     5581 gaagttgatt attaagttcg agactggttt ctaaactcat actgtggtcc tcgctacaag
     5641 gagaatgaaa gtgatgatca gaccgagcgc agtggcaacg gccagaccgg tcatcaaatc
     5701 gaagttttta cggcgataac ggagaacatc gcgccccgtc agtcgataga ggtgttcagg
     5761 tttcatcggt tgtgctccta aaacgcccat gcgctttttc ggctcggata cgtgcggcaa
     5821 tggcgctatc aagttcgggg aaatacccga ggtggagtgg tttaccgtta acggtgattt
     5881 gtgcccgcca acgctttctt gattctttcc agttgacccc cattgcgttt tgtggggcta
     5941 cgctgtgcaa ggtttttggc gttctcagcg cgtgtgacca gacgtaagtt ttcaatgcgg
     6001 ttatccgccc ggttatgatt gatatggtca atttccatac ctgcggggat atcgccgaag
     6061 tggtattccc agacgaggcg atgaacgtac tgattttttc catcaaggca gatggtgaca
     6121 taaccgtctg cttttacttt tcccgcgggc ttgcctgcca tgcgtgaatt agtggatttc
     6181 catgcgtggg ggcttttgaa atgcgatgcg ggtcggtatt tccatataag ctggccgttc
     6241 tggtattcaa aagcctccct gattctcttt ttcaacgtag gagtcctcgt gaaggtagga
     6301 atgaaaaaaa gcccgtcatg ggaggcgggc aaagactaca catagcaatt acatggatga
     6361 ttcagaagaa ttagttagcg gacggattgt gtagccataa ccgagatagc cgttgcgttc
     6421 ctggtaggcc gccgctctta gtcgggctgg gttttcgcct ttgacggttg caacgacgta
     6481 atcagtcgcg tcaggtccgt tttctaccac ttcaaaagcc ggagcgtatt ttgttgtgag
     6541 ctcataggta ttcatttcgg taccccttca gtggattagt aaaaggcccg aagcctttga
     6601 ttaattcact gcacgcccca tcatcggggc gtttcaattt acgtgactta tcagctcgtc
     6661 gcggtgttgt cctctacgct taccgtacgc atacggactc ggcgcttacc tcgatcccat
     6721 cgggtgccat ttcattttgc caggagtact gcggcttacc tgtcacgcgg ttcagtttgt
     6781 taaagagcga ttacttagtg cggttaaaaa tacaagcaaa cctgtaagtg cgtcaacagg
     6841 tattctggta ataaaatgca ttattaacct aattacttgt ttttacaggt aataaaaaaa
     6901 gagggcctaa gccctctttg tttagtagtc aggatgcgat tatctttttc tgcggaatat
     6961 gcgatgttct accattgtcc ctatgatctt aattggtttt tcccaggagc gacaaatagg
     7021 gtaatcacta ttcaatggaa caagttcaaa gtcttcctca cccttagcgc caatccctac
     7081 cgggcggtat tttttgaacg ttgcttcgtg gctaccgtta catgccgcaa cgaattcacc
     7141 aggagtaggg tatacgtcag tgtcaattat aacgatgtcc ccctctttaa attcaggttc
     7201 cattgaatcc ccacgtatac gtagcgcaaa tgaactttct gatagctcag ccgtggtgag
     7261 aatgtattcg aaatcacctt cttcacgaag tagctcagcg cttgtgaaag cccctgcttg
     7321 tacgtagctt agaacaggca cccttcgggc tccgtagttg aaatctgtaa caacatcgcc
     7381 cccaagaagt agccatttag gatcgcattc aagagctgac gctaaagcaa gaagattgcg
     7441 tggtttaagt gttttcccgt tctctatagc ttcaatcgac tgctggctaa tgcccgctcg
     7501 tttcgcaact tcgacctgag ttaagtttag ttctgttctg cgttttttag ctcggttggc
     7561 aagattcatg ttgactcctt ttctgtcatt gatgattaca gatacttctg tatttgacaa
     7621 acaaggttgc ctgtgaggta attacaagta aacctgtaat aatgagggct tactcaatga
     7681 ccagttccac ccttgcttca cgtataaagg agcgccgcaa agctttaggt ataacccaaa
     7741 cttcattggc tgaaagtgtt ggcatgcgcc agcagtctgt tcaatacctt gagtccggac
     7801 gcgccacacg taccggtttc atccttgaat tggcaaaagt tctgaaatgc gatcctgact
     7861 ggctattaaa cggcgaaaat tcagagcagc aaaaggtgta acccatgcct gataacaaac
     7921 cctggggagc tacgcccgat gagtggttcc acttcgatct ggtgttgggg atgacagaca
     7981 ggcttttacc tgtggtgtcc aatccctgtg cagaactgtc gcctaatagc aagctgtctc
     8041 ataatttcgg caaaactccc agtcgctaca atggccaacg taaggttgtt ggtatccccg
     8101 actgggctaa ctatgttgtt acggaaaacg attttgcccg gtggtcgaac gaacccgatt
     8161 acggtatttg cgttcgtact ggcgacgggg tggtggctct ggactgcgat atcaatgacg
     8221 ccgggatgca ggagatcgta cgcaatatta tcctgtcatg cctgggggaa cttccgccac
     8281 gtcgctggcg ggctgacagt cataaatgcc tgtacctgat tgctgttgat ggcgactacc
     8341 gtaaacgtgg tcaccgcctg gaaggcgaaa acaaacaaat agagctgctc gctaagggcc
     8401 agcagtttgt ggcttgtggt acccatccgg caggcgaacg tattcagtgg gactgcggtt
     8461 tgcccggcga acctctgaaa attacatctg aacagctcga aagcctgtgg cagcgtctgg
     8521 cggataactt acccgtgaaa gacagctata ccgcaggtgc aggacgtcag cgtgaccttt
     8581 cctgtgttga tccttccgcg acagatgatg tcgctgactg gcttgacgcg aacgggtgga
     8641 ctctgtcagt cagctctgat ggttcccgca atctgaaacc gtttcgggat gagtcagaat
     8701 acagcaacgg ttgcagcgaa acatccatta agtattttcc aaaagggacg gggggatttg
     8761 aactgggaca ttttaaaagc atgcacaaca ccgatgcggg gcttactgat gccgactggc
     8821 tggatgcata cggctaccgc gtggcggact tcgaagattt gacagtggct gaagatggcg
     8881 ataagcctga gtatcttccc tggcctaagt tcagtcgtga taagaatgac cggatagaag
     8941 gtaccgttac caatgtgctg ctggcgctgc ggcgaccgga tctctgcggt gtacaaatcc
     9001 gccttgatga gttccgggac gagataatac tgatcactcc acgggggaaa aacctgcggt
     9061 tgcgtgatga gttttatacc atgctgcact ctaggctcga atgtctggga ttcaagaaat
     9121 ttgaagaggc agcgatcagg cgtgcggtgc gcctggtcgc gtacgaaaac cgttttgatt
     9181 cgcttaaaga ctggattgcc aatcttcctg catgggatgg tgttccccgt atagatactt
     9241 tcttctgtcg gcactggggt atcgaggcca acgcctacac ccacgcagtc gggcgctact
     9301 ggtggacttt gctcgcgggt cgagctctgg ttcctggcat taaaggggac atggctgtga
     9361 tccttgtcag ccaacagggt aagaacaagt ccgaaggtat ccgtgcaatg gctccaaccc
     9421 ctgagcatta catggaactg gacttcggta agaagggaga ggagcgcatt cgtgagatgc
     9481 gagggcataa cgttattgag cttggtgaga tgcgcggtat ggataaacgg ggtattggtg
     9541 atgttcgtgt caccatatcc acccgtgcag accgcaaccg cgggttgttt cgggagcact
     9601 atgtcacttt accgaggcgt tgcggtttta ttggtacttc taactccgat acgccattga
     9661 cagataccga aggtaaccgc cgttggttgc cgatgacgat accggatgag actgacggca
     9721 agtttatcgc tcagggtatc gctgacgaaa aaatacagct ttgggctgaa gcagtcgccg
     9781 tttttaaacg tgatggcatc gcatgggaga acgctgaaac actggctaag tctattcttg
     9841 gggattatga ggtcaaagat gaggtctgga tgtcatgcgt cgctgagtgg ttagagttgc
     9901 cacaggatct gtacgatatg gctgccgacg gcacgaaaaa gaactgcgaa cgcgtccccc
     9961 ttacgtcaaa agacgtattg gtcgaggcga ttggcttcaa agccccccag gtaaaacgca
    10021 gtgacgaaat gaaaattgca gcaatcatga agaagcttgg ttacaaaaat aaacagttgc
    10081 aaagtttacc gggtaagcct cgcagctggg aaaaatgcaa ataactacaa gtttgtagat
    10141 ttttgcaggt tgttactcaa cctactcaac ctcacaaggg taggttgagt aagaaaaatg
    10201 gtattaaaat catgatatta ctcaaccact caacctactc aactatttta tataaatata
    10261 cgcgtgcgcg cgcacgcatg tatgtaagaa ttttattttt ccaaaaggtt gagtaggttg
    10321 agtaagagta tgatttttat gttttttagg ttactcaacc ttttgcccgt aaaaaaggtt
    10381 gagtaaagaa aacttgcaga aaatcacaaa taaccaaacg aagtaaaaac atgaatacaa
    10441 cctgtgcaag attaccaagt gcggatacgt tagcggaaca ggcagcaaaa acggcaagtg
    10501 ggtggatgtc agacgccgta atagcgatag atgagctttt tggaaagggc tatgcgaagc
    10561 aacaccctga attagttgct gcctttatgc aaacagcggg cttagatgaa gctgcaatgc
    10621 acttacgggg acttgtgtta gctaaccaac ggattgatga cacactggaa gtgctttcgg
    10681 agcatattat gctgcttaac gagccccaat aaacccgatg cggataaaca gacgtagttc
    10741 tcagaaaatt ttttcgtagc aaaacgtaga ggccagcgtt atgcgtagag atatgcaact
    10801 tgttctggag cggtgggggc gatgggcggc gagcgaggaa tactgttcaa tggttgactg
    10861 gccctcaatg tcggttactt cccgggacgt aaccgatagt ggcaaacccg gttgctctga
    10921 cgaggacgga gcggtgattg atacctgcat cgcgcatatg agtatggttt gcccgtacga
    10981 tgatttgctg atcctggggt tacgttttat aggcggcctc tcaacgcgtg caattgctga
    11041 ggcgatagac cgtagtcatc ttagcgtacg gacatccctg aaggcgtctg aagcatttct
    11101 ggaaggagct ctgactctgc agggagtaag gttggatatg gatcctgaag ttgtactacc
    11161 tgaaagggtt gtgtgcgcac aaaaaaatat gttatggtta tagcgtctat aattgtatgc
    11221 ttactgctga aaggttctga taacaccgga accttttttc ttgtcttctg ctgggaaaaa
    11281 ttaataaaac aggaaagaaa aacatgacat cgcactggtt cagtgtcagc cagtggcagt
    11341 taccaaacga ggatgattac agaaaacttc atgcgttgtt agctcagccg gacagagcaa
    11401 ttgccttcta agcaatcggt cactggttcg aatccagtac aacgcgccat atttatttac
    11461 caggctcgct tttgcgggcc ttttttatat ctgcgccggg tctggtgccg tattacttca
    11521 gccaaaagga atacctgtat atg
//
LOCUS       DAZGCQ010000140        11283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000140 DAZGCQ010000000
VERSION     DAZGCQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.140"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..767
                     /gene="ytfI"
                     /locus_tag="ACHK07_004435"
     CDS             <1..767
                     /gene="ytfI"
                     /locus_tag="ACHK07_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO8104684.1"
                     /translation="CATQIDKDSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEETPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDDFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(830..1384)
                     /locus_tag="ACHK07_004436"
     CDS             complement(830..1384)
                     /locus_tag="ACHK07_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8104685.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1709..1915
                     /locus_tag="ACHK07_004437"
     CDS             1709..1915
                     /locus_tag="ACHK07_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO8104686.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1977..3320)
                     /locus_tag="ACHK07_004438"
     CDS             complement(1977..3320)
                     /locus_tag="ACHK07_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO8104687.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3643..4281)
                     /gene="msrA"
                     /locus_tag="ACHK07_004439"
     CDS             complement(3643..4281)
                     /gene="msrA"
                     /locus_tag="ACHK07_004439"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO8104688.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4487..6220
                     /gene="tamA"
                     /locus_tag="ACHK07_004440"
     CDS             4487..6220
                     /gene="tamA"
                     /locus_tag="ACHK07_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO8104689.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6217..9996
                     /gene="tamB"
                     /locus_tag="ACHK07_004441"
     CDS             6217..9996
                     /gene="tamB"
                     /locus_tag="ACHK07_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO8104690.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRAALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMDVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDITKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9999..10340
                     /locus_tag="ACHK07_004442"
     CDS             9999..10340
                     /locus_tag="ACHK07_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO8104691.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(10623..10763)
                     /locus_tag="ACHK07_004443"
     CDS             complement(10623..10763)
                     /locus_tag="ACHK07_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8104692.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHSSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(10896..11198)
                     /locus_tag="ACHK07_004444"
     CDS             complement(10896..11198)
                     /locus_tag="ACHK07_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8104693.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPHLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGISSKPVHYARVPFG"
BASE COUNT         2855 a         2707 c         3036 g         2685 t
ORIGIN      
        1 gttgcgcaac tcagattgat aaagatagtt atcaagtgct ttcatcaaaa aatggaactg
       61 ttaacgctat ggataagttt cttatcagtt tcagccttaa agataacggt gccgaatata
      121 caatgacatt gcgaggcagt ggatttgaat acgaagaaac ccccataaca ataaatgagt
      181 ataactcatt catggatttc aaaaaccgtg aatttccgtt agaacaaaac agacgattgt
      241 atgcctggga tattctgcag aaaaaacagt ctgacatacc gaaaaggatt aaaggttata
      301 ttcaccaggc gattggtgac gtgtctttag gttatgcctt attggaggat atagtaagta
      361 aattaaaacg gggtaaattt gaactccaaa taccaggagg tggaattaaa gaatgcgatg
      421 gatggtatat ttacgaaaaa ataattgatg atgattttgc catagtcata gaatctttgg
      481 gctttgcgct taaaatctat ggaggggatg aacgttttcg taatggttcc tctgttgttt
      541 tggaggatga agattactct cttatttata attttctggt taatgcaggt tgccaacaag
      601 tcgaattagc cgaacaagtg gatgcgatag taagtgcgaa tctggccgca gatagtgata
      661 ttaccaaaga aaagatttgc gagaaatata aatcgaccat tgaggcgttt aaaaaagagc
      721 aactagcgtt accagtatta gttcggcgta agaactcaga gacttaattc ccctatccca
      781 tagataacga taggggaaaa ttatttttgc agaatttatg ttattgccat tatttgagca
      841 acttttgcag caggtccatc acttgctgca cctcttcctg agtgagcgcc ccgtctttgg
      901 cccattgcac gcgaccatcc ttatccagca ccactacagc ggaactttct tcatccagct
      961 gccaggcacc gcgcgcgacg ccattgctat cgacaataaa ctgcgaccag ggataaagct
     1021 ttttattact ctccagactg ctgcgcacaa acatgccgga acccggaatc gcgtcgtcgg
     1081 tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt
     1141 caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt tgcagtactc
     1201 gcacttttcc cactaactgc gcgctgttcc aggttttgta gctaaactga tctttatcaa
     1261 gcaccaactc gccccgatcg gtaatgccaa tcggcggcac tcgctgaccg gtttcgaact
     1321 gatgtgcgga agccatcatc ggcaacagca ggcaggtgag tgccagaatc ttgcgtaggg
     1381 tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct gagttttaat
     1441 catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca
     1501 aatcatatga aaaatgaatg cttatactga agaccgcgct tcggtaaaaa gataattctg
     1561 aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc
     1621 tatagtcact aagcattaaa atttgcgcct cataatagtt gggccgattg tggcaccgca
     1681 caggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca acccacttca
     1741 ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg acgttggcgc
     1801 ttttgaattt gataagggta agattcttat cccaaaagtg aaggataagc tgcatttgtc
     1861 agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa
     1921 gtgctatgca gtaataagac ggctcctgat tcaggagccg ttgatgttta tgggggttac
     1981 gcgacgcttt cttctttatc tttagcgtca ggcagttttg gcgaaagggc tgtggccttg
     2041 ctgtcgatcc gggtcaccag cagctggtcg atgcggtagt tatcgatatc caccacttca
     2101 aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg ggatcttacg cagcataaac
     2161 atcataaagc cgccgatggt ttcgtagttg cccgactgcg ggaactcgtc aatatccagc
     2221 acgcgcatga cgtcgtcaat cggagtgccg ccgtcaatca gccatgagtt ctcatcacgg
     2281 gcgacaatct gctcttccag tccctgaccg accagatcgc ccatcagcgt ggtcatcacg
     2341 tcgttgaggg tgatgatccc cactaccagc gcgtactcgt tcatgatcac cgcgaagtct
     2401 tctcctgcgg ttttaaaact ttccaacgcc tctgaaaggg ttaacgtatc cggcacaatc
     2461 agcgtgttgc gaatttgtac gccgctgttc agtgccaggc tttggttagc cagcacgcgg
     2521 ttcagcaggt ctttagaatc gacatagccg atgatgtggt caatatcttc attacagacg
     2581 aggaacttag agtgcggatg ttccgccacc ttatttttca gactttgctc atcttcgtgg
     2641 agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag acggaacggt acgggattcc
     2701 agctcaaaga cgttttcaat cagctcgtgt tcctgtttac gtaacacgcc cgccagcgca
     2761 ccggcttcca ctaccgcgta gatgtcatca gaagtgatgt catctttacg taccattggc
     2821 agtttgaaaa tacggaagat tatgttcgcc aggccgttga agaaccacac cagcggggtg
     2881 caaacgtaca ggcagaagcg catcgggttg atgatacgca aagccacagc ttctggcgca
     2941 atcataccga tgcgtttcgg ggttaaatcc gcaaacagga taaacatgcc agtcactaac
     3001 gagaaagaga gaataaagct cagttgctca gagagctctg ccgacatata gcgggagaac
     3061 aggctgtgaa aagctggaga aaatgccgca tcaccgacga taccgccgag aatcgccact
     3121 gcgttcagac cgatttggac cacggtaaag aacatgccgg gattttcctg catattcaga
     3181 acgcgttggg catttatatt gccttcatca gccagcagtt taagtttgat tttgcgtgag
     3241 gcggcaagcg agatctcgga catcgagaag aacgcactta cagcgatcaa gcagagtatg
     3301 actaaaatac tgtttaacat atcttatccg acctttcagg ccagatcctc ggaaggggaa
     3361 gttgattaat ttgtgtgaaa tacacattga aagccgattc gaagaaagtg aaccggcatt
     3421 ttcagtggct agtatagcgt aaggtactgt aaagtcgcca gagggttaaa attcagactg
     3481 ctgataaatg caatactgcc tgatgcgcta tgccgatcag gcctacgcag tccttgcaat
     3541 atattgaatt tggacgattt tgtaggcctg gtaaggcgtt tacgccgcat ccggcataaa
     3601 taaagcgcac tttatcaaca atctacattt gtacccgtaa cgctatgctt ccggtggcag
     3661 acagacgcca attccgccaa ttccacagta accatacggg tttttatgca gatattgctg
     3721 gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg tgatgtgacg
     3781 atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc gagctgcggc
     3841 atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt ggtcattgcc
     3901 ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acctgtagca actgctcata
     3961 gctgatgacg gaaggatcgt aaacaatgcg taccgcttcg gcatgacccg tatcaccgga
     4021 gcacacttcc cgataagtcg gatttggcgt gtagccgccg gtatagcctg cggcggtgct
     4081 gtaaacgccg ggtaactgcc agaacagacg ctccacaccc cagaaacaac ccatcgcaaa
     4141 aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttga ccgcatgcag
     4201 cgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg aaaccagatg
     4261 ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg gggttaaggt
     4321 tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa gtaaaacata
     4381 agaaatattt ggggtttagt ctgcttttta atccatatta ctggattttt gttaagccgt
     4441 ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc gctatatccg
     4501 acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg cgaacgtccg
     4561 tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgcgcgc agctttctac
     4621 gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg atgatgctat
     4681 ccgcgaaggt ctgaaagcgc tgggttatta ccagccgacc attgaatttg atctccgtcc
     4741 accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggcg tgccggtgtt
     4801 aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg accgataaag actatttgaa
     4861 attgctcgat actcgcccgg ctattggtac ggtactgaac cagggcgatt atgaaaattt
     4921 caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat ttcgatagcg aatttaccaa
     4981 agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg attataacag
     5041 tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc gcgatgaata
     5101 cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac gaatcgaaag atctggcaga
     5161 actgaaccgc cgactttctg ctaccggctg gtttaactcg gtggtggtgg ctccacaatt
     5221 tgataaagcg cgcgaaacga aagtattacc attgacgggc gtggtttcgc cgcgaacaga
     5281 aaacaccatc gaaaccgggg tcggttactc tacggacgtg ggaccgcgcg tgaaagcgac
     5341 gtggaaaaag ccgtggatga actcttatgg tcacagtctg accaccagta ccagtatttc
     5401 cgcgccggaa cagaccctcg acttcagcta taaaatgccg ctgctgaaga atccactgga
     5461 acaatattat ttggtgcagg gcggttttaa gcgcactgac ctgaacgata ccgagtctga
     5521 ctccactacg ctggtggctt ctcgctactg ggatctctcc agcggctggc agcgtgccat
     5581 taacctgcgc tggagtctcg accactttac tcagggtgaa attaccaaca ccacgatgct
     5641 gttttatcct ggggtgatga ttagccgcac gcgttctcgt ggtggcctga tgccaacctg
     5701 gggcgactcg caacgctact ctatcgacta ctccaacact gcctggggct cagatgtcga
     5761 tttctccgtt ttccaggcac aaaacgtctg gatccgcaca ctgtacgatc gccatcgttt
     5821 tgtgacacgc ggcacgctgg gctggattga aaccggtgat ttcgacaaag taccgccgga
     5881 tctgcgtttt ttcgccgggg gcgaccgcag tattcgtggc tacaaataca aatctatcgc
     5941 tccgaaatac gccaacggtg acctgaaagg ggcctcgaag ttgataaccg gatcgctgga
     6001 gtaccagtac aacgtgaccg gaaaatggtg gggcgcggtg tttgtcgata gtggcgaagc
     6061 ggtaagcgat attcgccgca gcgactttaa aaccggtacc ggggtcggcg tacgctggga
     6121 atcgccggtc gggccaatca aactcgattt tgccgtaccg gtcgcggata aagacgaaca
     6181 cgggttacag ttttacatcg gtctggggcc agaattatga gtttatggaa aaaaatcagc
     6241 ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg tggcgtttct ggtgggcacc
     6301 accagcggcc tgcatctggt atttaaagcg gcggatcgct gggtgccagg actggatatt
     6361 ggcaaggtca ccggcggctg gcgcgatctc accttgtctg acgttcgtta tgagcagcca
     6421 ggcgtggcgg taaaagcagg caatctgcat ctggcggtcg ggcttgagtg cctgtggaac
     6481 agcagtgttt gtattaatga tctggcgctg aaagacatcc aggtcaatat cgacagcaaa
     6541 aaaatgcccc cttctgaaca ggttgaagaa gaggaagata gcggtccgct ggatctctcc
     6601 acgccgtatc ccatcaccct gacacgggcg gcactggaca acgtcaacat caagattgat
     6661 gacaccacgg tatcggtgat ggacttcacc tccggcctga actggcagga gaaaaccctg
     6721 accctgaaac cgacgtcgct gaaaggcctg ctgattgctc tgccgaaagt ggcggaagtg
     6781 gcgcaggaag aagtggtcga accgaaaatt gaaaatccgc agccggagga aaagccgctc
     6841 ggcgaaacgc tgaaagatct cttttctcgc ccggtattgc cggaaatgac cgacgtgcat
     6901 ttgccgctta acctgaacat tgaagagttt aagggcgagc agttgcgcgt gacgggcgac
     6961 acggacatca ccgtgcgcac catgctgctg aaagtgagca gcattgacgg caatactaaa
     7021 ctggacgccc tggatatcga ttccaaccag gggatcgtca acgccagcgg cacggcgcag
     7081 ctgtcagaca actggccggt ggacatcact ctcaacagta cactgaacgt ggagccgttg
     7141 aaaggtgaaa aagtgaagct gaaagtgggc ggcgcgctgc gcgaacagct ggagattggc
     7201 gttaatcttt ccggtccggt ggatatggat ttacgcgccc agacgcgact ggcggaagcc
     7261 ggattgccgc tcaacgtgga agtgaacagc aaacagattt actggccgtt cactggtgag
     7321 aagcagtatc aggcggatga tctgaaactg aaacttaccg gcaaaatgac cgattacacg
     7381 ctctctatgc gtacggcagt gaagggacag gagatcccgc ccgcgactat taccctcgac
     7441 gccaaaggta atgaacagca ggtcaatctc gacaaactca ccgtcgcggc gctggaaggg
     7501 aaaactgaac tcaaggcgtt gctcgactgg cagcaggcca ttagttggcg cggtgagcta
     7561 acgcttaatg gcattaacac cgccaaagag ttcccagagt ggccgtcgaa actcaatggg
     7621 ttgattaaaa cccgcggtag cctgtacggc ggcacctggc agatggacgt gccggagtta
     7681 aagctgaccg gtaacgttaa acagaacaaa gtgaacgttg acggcacgct gaaaggcaac
     7741 agttatatgc agtggatgat cccagggctt catctggaac tcgggccaaa cagtgccgaa
     7801 gtgaaaggcg agctgggggt aaaagatctc aatcttgatg ccaccatcaa cgcgccgggg
     7861 ctggataacg cgctgccggg gcttggcggt acagcgaaag ggctggtgaa agtacgcggc
     7921 acggtggaag cgccacaact actggcagat atcaccgcgc gcggcctgcg ctggcaggaa
     7981 ctttccgtgg cgcaggttcg cgtggaaggc gacattaaat ccaccgatca gatcgccggg
     8041 aaactcgacg tacgcgttga gcaaatttcg cagccggatg taaatatcaa cctcgtcacc
     8101 ctgaatgcca aaggcagcga aaagcagcac gagctacagt tgcggattca gggcgagcct
     8161 gtctccgggc agcttaatct ggcaggaagt tttgatcgca aagaagaacg ctggaaggga
     8221 acacttagca atacccgctt ccagacgccg gtcggcccgt ggtcgctgac ccgcgatatt
     8281 gcgctggatt accgcaataa ggagcaaaaa atcagcatcg ggccacactg ctggcttaac
     8341 ccgaatgcgg aactgtgcgt gccgcaaact atcgatgcgg gggccgaagg gcgtgcggtg
     8401 gtgaatctca accgcttcga cctcgccatg ctgaaaccgt ttatgccaga aaccactcag
     8461 gccagcggta tcttcacggg taaagcggac gttgcctggg acatcacgaa agaggggctg
     8521 ccgcagggca gtatcactct ttcggggcgt aacgttcagg taacgcaaac cgtcaacgat
     8581 gcggcgctgc ccgtggcgtt tcagacgttg aatctgtcgg cggagttgcg taacaaccgt
     8641 gccgaattgg gctggaccat ccgcctgacc aataacggcc agtttgatgg acaggtgcag
     8701 gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg tcaatatccg taacttcaac
     8761 cttgcgatga taaaccccat ctttacccgt ggggaaaaag cagcggggat ggtgagtgcc
     8821 aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt ttggtcagct tcaggttacg
     8881 ggtgtggata tcgatggcaa ctttatgccg tttgatatgc aaccgagcca gcttgcggtc
     8941 aactttaacg gtatgcgctc gacgcttgcc ggtacagtac ggacccagca gggtgaaatc
     9001 tacctgaacg gtgatgccga ctggagccaa attgaaaact ggcgggcgcg ggtaacggcg
     9061 aaaggcagta aagtgcggat caccgtgccg ccgatggtac gaatggatgt atcgccagat
     9121 gttgtattcg aggctacacc aaacctgttt accctcgatg gtcgcgtgga tgtcccgtgg
     9181 gcgcgcatcg tggtgcacga tctgccggaa agcgcagtag gcgtctccag cgatgtggtg
     9241 atgcttaacg ataacctgca accggaagag ccgaaaacgg cgtcgattcc gattaacagt
     9301 aacctgattg tccacgttgg caacaatgtg cgcattgacg cctttggcct gaaagcgcgg
     9361 ctgacgggcg atcttaacgt cgttcaggac aaacaagggc tgggcctgaa cgggcagatc
     9421 aacatccctg aagggcgctt ccatgcctat ggtcaggatc tgattgtgcg taaaggtgag
     9481 ttactgttct ctggcccgcc ggatcaaccg tatcttaata ttgaagctat tcgtaacccg
     9541 gatgctacag aagacgacgt aatcgccgga gttcgcgtca ctggtctggc ggacgaaccg
     9601 aaagcggaga tcttctctga cccggcgatg tcgcaacaag ctgccttgtc ttatttgcta
     9661 cgtggacaag ggctggagag cgatcagagc gacagtgcgg caatgacctc gatgctgatt
     9721 ggtctggggg ttgcgcaaag tggccagatt gtgggtaaaa tcggcgagac gtttggcgta
     9781 agcaatttag cgctcgacac ccagggagta ggcgactcct cccaggtagt ggtcagcggc
     9841 tatgtattgc caggtctgca agtgaaatac ggcgtgggta tatttgactc tatagcaaca
     9901 ctcacgttac gttatcgcct gatgcctaag ctatatctgg aagccgtgtc tggtgtagac
     9961 caggcactgg atttgctcta tcagttcgag ttttagcaat gcgaatattt gtctacggca
    10021 gtttacgcca caaacaaggc aacagtcact ggatgaccaa tgcccagtta ctgggcgatt
    10081 tcagtatcga taactaccag ttgtatagcc tgggccacta tccaggcgca gttccgggga
    10141 acggaacggt acacggtgaa gtttatcgta ttgacaacgc cacgctggcc gaacttgatg
    10201 ccttgcgcac caggggcggt gaatacgcgc gccagttgat tcagacgccg tacgggagtg
    10261 catggatgta cgtttatcaa cgacccgtcg atggattaaa gctaattgaa agcggcgact
    10321 ggttagacag ggataagtaa ccctatgcat acgccacctt cgggtggcgc gaaaattgat
    10381 gtaatacaat gaaaaataaa ggattatttt tcgagttgtc cacatgttgc catggcgttc
    10441 taaatttttt tggtgagact atctctgctt gttgttttca ccgccagctt gttaaacaaa
    10501 aaaagcccac agtttttacg ctgcgggctt tgtttttctt tgtgacttgc gtgtgagggt
    10561 gcgactttcg accgaacctt cggcaaccgg atagcggggg cgttcacccc cgctgcggtt
    10621 tcttacggtt tacactgcaa gaacgccgca aattctgacc acatgatgtt aagcctgaac
    10681 tcacacggcg aactgtgtat catccagaca gtcaacaaca tggttaagca taaaatgctc
    10741 acgatgatcg ttttttgcgg catagcgctt gctccttttt caaagaggcg ctaaccttcc
    10801 acttgtctaa ggtaggatgg ttagggcctc gggttaaaca atatgtttga ctcggggcct
    10861 ttccacatcc ggccttcagg ttttccttcc agccgtcagc cgaaaggcac ccgcgcgtaa
    10921 tgtactggtt ttgaggatat gccgcaatcc tccagttgta gcgttaactg actgcgtccc
    10981 tgatgggatt tgcgcattgt ctcccgtgga atatctttat cgtggaagat caaatgaggc
    11041 tggccaataa ttctgtgcac actttccatt gttgaaaatg tggcgctgca ctcaaggtta
    11101 ttgcactgat agtatttgta gcggatggtg ccggtatcat tggcggcttt actggtacgg
    11161 atccgggcag atgcgccgca gaatggacaa tgaaacattg atataccccc tggctgaact
    11221 ggggttgaac tcgtcgccat tctaaccatt gctttccttt tcggctatcc actcgggtat
    11281 tct
//
LOCUS       DAZGCQ010000141        11157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000141 DAZGCQ010000000
VERSION     DAZGCQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.141"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..1960
                     /gene="iolC"
                     /locus_tag="ACHK07_004445"
     CDS             23..1960
                     /gene="iolC"
                     /locus_tag="ACHK07_004445"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFO8104694.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
     gene            complement(2722..4293)
                     /locus_tag="ACHK07_004446"
     CDS             complement(2722..4293)
                     /locus_tag="ACHK07_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO8104695.1"
                     /translation="METSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPALPS
                     ALRQSSARKPLPTTLPRETRVIRPEEECCPACGGELSPLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     DVELSRATLGRWTGAVAELLEPLYDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTAW
                     LWVYVRDDRNAGSEMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRVLYESG
                     RITEAACMAHARRKIHDVHARVPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCGNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLTSQ"
     gene            complement(4313..4660)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004447"
     CDS             complement(4313..4660)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8104696.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKIHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(4660..5307)
                     /gene="tnpA"
                     /locus_tag="ACHK07_004448"
     CDS             complement(4660..5307)
                     /gene="tnpA"
                     /locus_tag="ACHK07_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO8104697.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSTPTVTQPVKAEKPRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPDPVQVEQINTPTDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
     gene            6098..6268
                     /locus_tag="ACHK07_004449"
                     /pseudo
     CDS             6098..6268
                     /locus_tag="ACHK07_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6484..7317)
                     /locus_tag="ACHK07_004450"
     CDS             complement(6484..7317)
                     /locus_tag="ACHK07_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8104698.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(7324..8661)
                     /locus_tag="ACHK07_004451"
     CDS             complement(7324..8661)
                     /locus_tag="ACHK07_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFO8104699.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(8759..9769)
                     /gene="iolG1"
                     /locus_tag="ACHK07_004452"
     CDS             complement(8759..9769)
                     /gene="iolG1"
                     /locus_tag="ACHK07_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFO8104700.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(9787..10692)
                     /gene="iolE"
                     /locus_tag="ACHK07_004453"
     CDS             complement(9787..10692)
                     /gene="iolE"
                     /locus_tag="ACHK07_004453"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFO8104701.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         2887 a         2859 c         2499 g         2912 t
ORIGIN      
        1 acacggtctt ctgtaggagg atatctctat gaacaacaca gtcagcagtg agcaaaaaac
       61 gctggacatt atctgtctgg ggcgtgtcgc cgttgattta tatggtcaac aaatcgggtc
      121 acgtctggaa gacatgacaa cctttgcaaa atatttagga ggctcatcag gcaacgtcgc
      181 ttacggcaca gcaattcagg gactacgttc cgccatgctg gcacgtgtgg gcgatgaaca
      241 tatgggacgt ttcatccgcg aagaattaaa ccgtgttggc gtcaatacac aatgcctgtt
      301 gactgatcct gaacgtctga cagccctggt gatccttggt attaaagatc aggacacctt
      361 cccgctgatc ttttatcgag ataactgtgc ggatatggcg ctggcgcctg aggatatcca
      421 cgaaacattc attgcctctt cacgtgccct ggccgtcacc ggcactcatc tttctcacat
      481 caatacccgc gccgcagtgc ttaaagcgct gacctatgct caaaaacacg gtctgcgtag
      541 agtgctggat attgattatc gtccggtact gtgggggttg acatcgctgg gggatggtga
      601 aacacgtttc atcgcttcac cacaggtgac tgaacagctt caacaggtat tgcaccattt
      661 tgatctgata gttggcaccg aagaggaatt tcatatcgcc ggcggcagta ccgatacgtt
      721 gaccgccctg cgccgtgtgc gtcagctaac ctctgcggta ttggtatgca aatgcggcgc
      781 attagggtgt tcagtatttg aaggaaacat cgccgacgac tggtctcagg tgcagatgca
      841 cagcggtgta cgtgtggaca tcctaaatgt gctgggtgca ggagacgcct ttatgtcggg
      901 actgcttcga ggctatctga acgatgaagg ttgggagcaa gcttgtcgtt atgctaacgc
      961 atgtggcgca ttagttgtat cgcgccacgg ctgcgccccc gcgatgccaa ccaaacagga
     1021 actggatgac tatctctccc gtgagcacgc tatcaccaga cccgacaaag accctcacct
     1081 taaccatctt caccgaacga caacccgaaa acagcactgg ccggaactgt gtgtattcgc
     1141 ctttgaccat cgcaatcaac tggtggatat cgccagagcg acaggtgcca gcgagttcac
     1201 tatccctgag ctgaagatgc tactactgga aggcgcacgt caggcggcgc acgaggctgg
     1261 attactgcat aactgcggca tcctggcgga tactactttt ggtcagaaag cacttaacga
     1321 cgtgactggt cagggatggt ggattggacg tcctatcgaa atgccggggt catatcctct
     1381 gaagctggag cacggtaata tcggttcaca acttgtcagt tggccgaaag agcacgtcgt
     1441 gaaatgcctt gtcttctatc acccactgga tgcagaatcc ctccgcctta agcaagaagc
     1501 actcattgat gagatatacc gggcctgctg ccagtctgga cacgatctgc tgctggaggt
     1561 catcttgcca cgcgacgcag cagatcaaag cgagcattac tatacacaga ttgtggaacg
     1621 tttttacctg ctggggatcc agcctgactg gtggaagctt ccaccgttaa gtccggctcg
     1681 ttggcaagaa atcagccagc atattgagcg ctacgatccc gaatgccgcg gtattttaat
     1741 acttggactg gatgcaccgg aaagcgagct gaaatctggc tttgccgccg ctgcggatat
     1801 accatgggtt aaaggcttcg ccgtaggtcg caccatcttt ggcgagccat cacggcagtg
     1861 gttcagcggc cagattaacg atgaacagct catcgcagca gtgaaacaaa aataccgcac
     1921 attgatcgac tactggcgcg aatatcgtcc ggcgctttag tatccgtctg cattacgtcc
     1981 cgcgtctgta ttcttgctcg gggcgtattc aaagatgttt catgaccgtc ctactttcct
     2041 catcatcccg acagtgctgt ttcgtatctg cattaatgat atttctgaac tgtctggcta
     2101 ttggtagttc cctcgtcacg ttgccgcaga acgtacaaca gctgtaccat ctgagcaatc
     2161 ttctgatcgg tacgctacag gcagcttgac ggttaatttc cattttactc aaccgagggg
     2221 tggcagaacg aaaaaacgac acctcagacg ggcgacagac ggttatccgt ggccagggtc
     2281 aggtcgcctg tgctgaatca gctccctcat cttgctggta atcctgcttg attaacccgt
     2341 tccactactg tcatggagtt tatattaacg catcccgttt ttcacagctt ccattgttct
     2401 gacattgacg gtaggttgca gctttatatc cgtacatgga ctcccgattt tgtgcaaaaa
     2461 agatgcttca gttggggttc agactgcccc cgtacattcg gcttcatttt acagtttttt
     2521 gcagagccat gacggatatc tgcgcaccta ttccttatcg gctttatgac ctttgatggt
     2581 cgtcggatat atcatatttt gctggtgtcg gtctgaccga tcttccgtct cactttcctg
     2641 cgcaattcat ttcagtaggc cacttcagac aaacttttct ctacaacata cgtaagcggc
     2701 tcgccagaac cgtattgata tttactgaga ggtcagatca actttccagg gcaacagatc
     2761 gcgtacccgg tttgccggcc agtcctggat atgctcaatg acgtaacgca gccatttttc
     2821 tggttccaca ttgttcagac ggcatgtgcc gatcagcgag tacaacaccg ccgcatgctc
     2881 gccaccgctg tcggaacccg cgaagagcca gtttttccgg cctacggcca ctccccgtaa
     2941 ggcgttctct gcgatgttgt tgtcgatttc cacccagcca ttaccgcagt acacgttcag
     3001 gccatcccac tgtttcagca ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     3061 tgttttcatc tgagtctgta tccagtcata cagtgactgc atcagtggcg cagctctggc
     3121 ttttcttgcc gccagacgct gttctgctgt acatccccgg acttctgcct ctatggcata
     3181 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gtgggcactc ttgcatgcac
     3241 atcgtggatt tttctccggg catgagccat acacgcggct tccgttattc tgccggattc
     3301 gtataacacc cggtaaccac cgtaagcatc ggcctgaagc acaccgctgt aaccggccag
     3361 atgattttgt ggatggatac ctttccggtc cggtgagtac gcgaaccaga ccgccggggg
     3421 catttccgaa ccggcgttac ggtcatcacg gacgtagacc cacagccagg cggtccgggt
     3481 tttaccactg cccggctcct ggaccggaac ggggatatca tcggcatgga ctttaccggg
     3541 catcagcaca tactggcgca ggatgtcata cagcggctcc agcagttcag caacggcacc
     3601 ggtccagcga cccagcgtgg cacggctcag ctccacgtcc tggcgacggt atatttctga
     3661 ctggcggtat aacggcagat ggtctgcata cttcccggtg acaacatggg ccagaagccc
     3721 cgctccggca taactgcgtg caatgggttt tgaaggcacc ggtgcctgca caatatggtc
     3781 gcaccgacaa caggccagtt tcggacgttg tgtttcgata accttaaagg cgctgctgat
     3841 aagctccagc tgctctgaca catcacatcc cagtggactg agttcaccac cacaggccgg
     3901 acagcattcc tcttccggcc ggatgacccg ggtttcacgg ggaagtgtgg tcggtaacgg
     3961 tttacgggct gaagactggc gcagggcgga tggcagtgcc gggtcatatt gttcacccag
     4021 cgtttccgcc atttcttcct gaagtgcgct gattcgctcc tgtgcttcct gtatctgccg
     4081 ttcggttttt gcacgaagtt tttctgagct tttaccgaac tgcatacgct gcagtttcgc
     4141 aaccagcgcc ttcagccggt tgatttcaga agcataagcc gccaccctct gtgagagcag
     4201 gcggttgtat tcggccatct ggcggatggt gtcctgttgc gtctgcaaca gtgcccgcag
     4261 gcgggcgttc tcatgagcaa gtgaggtctc catatcctca ctttacaacg ggttatatgc
     4321 ggattccagc gcgttccgtt cgttttggat gtttccagtc gatgccttca agaagcatgg
     4381 acaactgagc cggagtaagg tgaatctttc catcgcgggt gaccggccag acgaagcggc
     4441 cccgctccag gcgtttggtg aagaggcaca gtccgtcgct gtcagcccac aacactttta
     4501 tctggtcacc ccggcgtccg cggaagatga acagatgccc ggagaacggg tcatccttca
     4561 ggacgttctg aacttttgat gccaggccgt taaaaccatt tcgcatgtcg gtgataccgg
     4621 caaccagcca gatacgcgaa ccggcaggga gagatatcat cagtggctgc tccctttcat
     4681 ttcgcggata agtgtctgta ataacgccgg tgtcagttca cctttcagcc tgagagttcc
     4741 ggccggcaga accagctcgc agcacagact gtcggtcggt gtatttatct gctcaacctg
     4801 cacggggtcc gggattttat tatccggctc cggagttaac gtcacgggaa gtagcgatgt
     4861 ttcatgttcg ccgggcggct gcaggccgcc gttacgccag agatgccgcc agttaaagag
     4921 caggttatcg ttgattccgt tctcacgagc tatttgtgcg acacaggcac cgggtaaaag
     4981 agtctgctga acaagtctcc atttaaactc cggtgaaaat cggttccggc ggggtttctc
     5041 tgctttcaca ggctgagtca cggtcggggt tgaaggatat cgccccggga aaagagcccg
     5101 ggccagttgc ccgcaggaat attcaccgga aagaggccag tcagtgaata cctggtgaaa
     5161 tcgcctcacc agagaaaaaa tggcggtttt gctgacacca gtctgcgcag aaatgcgcgg
     5221 gtacgacagt gactcgtcaa atctcagctg tagcacacgc aggatcaatt ccggattatc
     5281 tgccagtacc gcctttcgtg cattcatctt aaatgtcctt ttactgcgaa aatggacatt
     5341 agtatcggaa acaggaaaag gaggcgaaag acggtttaaa tgatgcgctt accaacatac
     5401 tttaactggt tgctgatata agtttttcac cacgcataaa cactgcccat gcgatcctgg
     5461 caatcttatt tgccagctct atgatgacga tattcgttgg tcgacgtgcc tgctgggctt
     5521 aaatctattc ccccggggat ccggctttat tgcaaccata aagctgcgag cccctatgta
     5581 attcgctgac tcacacctca tttactgcgc gggattcaag cccaaaaccc catttaggtt
     5641 tagtgagtcc ctgtcttacc tgtctttgtc atcacgttga ccaccagagc aacctttctg
     5701 cattcaccca tttaagtaac caaaatgagt atcaggcctg atatatacag aactgaattc
     5761 gttaattaat tattaatcag ttaaattttc cgttactggg atgaggtgta cacttggcct
     5821 ctcccaaaat ccgcggaacc gcccctggcc cctattcagc atttcaggct ctaagttgag
     5881 gactgaccgg attgacatag ggcctgctga cgccattcat tgactaccaa cagatctttc
     5941 ttgttattta tagaaaacca ccagccagcc tagtggttca gtaaaacttc caactttgag
     6001 gctgttgtca aaaccccttt ttgatttgtt aaaacacctt gcagtctacc aactgtaagc
     6061 gtcaaacaag aaatcaaaag gggattccaa tgggtgaatg aaaaagggct taacgaacac
     6121 ccgatggaac tgtaaatatc acatagtttt tgcgccaaaa taccgaagac agatgttcta
     6181 cagagaaaag cgaagtgcaa gagcaccagg caatattttg agaaaactgt gtgagtggaa
     6241 aagtgtacgg attctggaag ctgaatgctg agcggatcat atccatatgc ttgtgaattt
     6301 acggggcgta agtaaggaag ttgaatgaaa atgtcagatc atatgcgcct gtcagccagg
     6361 gcgaggcaga aaacgaatct ttaagacaca tctgaaaaac ctccggctat gccggaggag
     6421 atttattctg atcacctgct tattaccggg cacagcgaca gttcatttgc agttacgtca
     6481 gcgttaatcc gctaagaaag gtcgcgtttt ttccgctccg atagcaaaga cctcgctggc
     6541 tgccatttgc cagtaacctg ggttaaacaa ctccagtgaa cagatccctg tataaccttt
     6601 tttcaccagt tcatgggtga tcatgtacag tggtatcgca ccgttgcctg gaaacagacg
     6661 atgacaatga tccaacacag ctacaggtaa acggtcagca tcatcaatat gatagacaaa
     6721 gatcttttcc accggaacct gtcgcagcgt actcagatcc tgccattgat cgtacagata
     6781 aaggttaaag gcatctattg ccagaccaac gttactccga tcaactgcgt ccacgatctc
     6841 catcgccatc gccagactgc gtacgcaaca tcccgcagag ccgataggct caaaggccag
     6901 tcgcatctgg ctctctgccg ctaatgctga catctcacgc aaacgtttca ctgaatctct
     6961 taccgtgtca cgatctgaaa aatgagcacc ttcacgaatg gtcggcacta caaccaggca
     7021 atcaccacct acgacttccc ctgcataaca ggctaaaacc agttgttgcc gtatttccga
     7081 ccagcgcccg gcatcacaaa atgtgatgtc ttccagcgag ttgtagcccc atggcttaag
     7141 atggcttaca gaaaaaatat cggccagttc aaccagagtg tgttcctgca gccacgtctg
     7201 caacatatcc agacggatct caatatagtc atatccaaaa cgttccgcta ggaccagatc
     7261 cttctccagg ctggaattac gcagacaggt cgcttcattg aatcccagtt tcatcattca
     7321 gacttactcc tgccgccaca ccaatgtact ggctccagaa ggcggcagcg ttaatgcgag
     7381 ttctctgtcg ccatcgacca cccggaaatg cctttctcgc gtatcgcgat tatataccac
     7441 taaaactcgc tcaccatcag gattaacaaa acccacatct tccagttgcg tatcataact
     7501 ggaagtaagc ataactttcg caccgggatg cacaaagcgg caaaagtgtc caattccgta
     7561 ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg tactgcacag gagcttcgca
     7621 caaattactc tggtgatttg ggcctccatc actgtctaac agcaggttcc agtcgataaa
     7681 tccgctacag ccggatttaa aattaccaat catgtcatga atataggtat gccagtgacg
     7741 gatctggcca ccttcatcat tctccattgg tacacatcct tcggaaaaca gcagcttttt
     7801 attcggcaac ttccgggcca gatactgaat ttgcgaaaaa tggtcaccgg tataccagtg
     7861 gaaagccatc ccgttgatgc tatcgccatc cttttcgtca gcaaaggcac gttcagccca
     7921 gtcaatcaga ccatctttat catgatccca gatatagatc tctatttcat tcattccata
     7981 gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta gtctcttctt ctacactata
     8041 cagacaggaa tcccagcttt ttatcgcaac tggttcattc tgcactgata gtgctttaac
     8101 actgatacca tggcgtctgt attcgagcag atagtcgata ataatatctg cccagtcagc
     8161 atagtattca taccgcaatt tcccacctcc gttcatatca taattagtct tcatgaatgc
     8221 cggtggactc cacggggaag ccattaattt tatttgtgga ttgatccgca atgccgcgga
     8281 aattagcggc attaaatgag attcatcacg ggaaaaggaa atccttccct gacgcaggtc
     8341 ttcactggaa tcaacatagg cataattgct cagagagaaa tcacagcttt ggatcggcat
     8401 ccgccccaaa ctatagttat gttcctgagg cgaaaaatag agattcaaaa attgcgcctt
     8461 tgtcttatca ctcatgctgt tgaaaacaac accagcactt tcagtgaagc tgccgccaaa
     8521 tccatcaatc tgttgtaacg agtggtttgg aaagacgctg attaattcac tacacgctgg
     8581 ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat tgctgattcc agggatcgga
     8641 agtgattagt ctgcctttca tagagggatt ccagataata taggatgata aattgtgata
     8701 taccggacag gagcatacac ctagctatcc ggtatgaaat tatcacatcg cagaaagact
     8761 atttgtagaa gtcaggtttt gctggtaatt ccacaaactc ggtgtttcct gtttcctggg
     8821 acttcacaca ggcatcagcc gttaccgcag ccagataacc atcccatgat gttgggcctg
     8881 ccagaggtaa tcccgcattc agacggtcaa agaagtcctg gaactcaata tcataggcat
     8941 caataaagcg ttgtttccag tccaccaaaa tgtcagtact gtattttgcc tctttacgca
     9001 ccgcagcagt agccacggta ggtaattcca ccatcccttt ctcacaggtc acatcacaat
     9061 gaatatcata gccatactgg caattcacaa acacctcaac cactatatta ataccggagg
     9121 tcgtttccat caccaccacc tgcggatctc gtagagtagt caccaagctg gactgacgtg
     9181 ggaagtagac cttaactgtt ctataatctt cgttgagtaa ccagtgcata acatcgattt
     9241 catgaatcag cgtttcataa attgcttgtg gtgttttgta atccgatacc gtgcttacat
     9301 tataatgacg accatgaacc attaatggtt gaccaatttc tccgctatcg atgatgtttt
     9361 tcagctgtac gtagccttta tcgtagcgac gcataaaccc aacttgcacc attcgttttc
     9421 catttttacg ttcagctccg atcacacgct gacaatcatc tgctgtaacc gcaagcggct
     9481 tttcacagaa aacatattta ttggcattta acgctgcaac ggctacatcg gcatgtactt
     9541 cattcgaggc agtaattacc actacttcga catcttcatc attaatcaga tcgtgatagt
     9601 ctttatagtc ttttgctttg atctcatact tatccaaagc tgcttgcgct ctgccttcga
     9661 caatatcgca cacagcaaca acttctactc cagaaatagt gttagccaga cgtcttaagt
     9721 gatcggcacc aattttcccg atccccacaa tacctgcttt taaagtcatc ttattttcct
     9781 tttctatcag atataattac ccaataaacc atcaatatgc tttttacctt tttgtgcata
     9841 ttccaaagga ttatatatat tcgggtcctg ctcagcttca acgacaatcc agccggaata
     9901 atgattctca accaaataag ccaatacaga tttaaaatca atattcccat caccaggaac
     9961 agtaaacatt ccatcaagaa aagaattgag aaatgatttt tttccagccc gacatgcttt
    10021 tagtttttca tcccgaacat ctttaaagtg aacatgttta atacgggaaa aatgcttgca
    10081 caacagtgac atcacatctc catcagaaac ataaatatga ccagtatcaa acagcaaatt
    10141 cactaattgt ggatctgtgt tttccataag tcgatccact tcctgtaaag tttgtacgcc
    10201 agtgcccata tgatggtgga aagcaagttt aagcccatat gtattggcga tcatgcctaa
    10261 ctcgttcaat cctgagcata attgttgcca ttcgatatca ttgaagtaag gtttttccgt
    10321 atagacacat ttatcaataa ttccttgaat actatacgtt tgctcagaca caacagcgat
    10381 ataagcatgg acagcctgca aataagcgca atgttgttca aaagctttct tcactgattc
    10441 aataccatca cgaataataa aagatgagaa ccactttccg ggaatttcca gatggcgaag
    10501 tgctaactct atatttaatt cagtcgggtc cagaggataa catcccccca cttctgttcc
    10561 agaaaaacca gtaagtgctg catcacttaa tatctgctta taggtattgt ctttaccaat
    10621 ttcaggcaaa tcatcatttc gccatccgat tggcgcaatc ccccatttaa tactctcttt
    10681 tacactatac atattccaga ctcctgctta ataaaacttt ctcacatcct gttgcataag
    10741 taataatctt tggctgacaa tatgctactt tccacgtggc taaaaaactt tatctatcat
    10801 gagtacagaa gtgggaaaaa tgtgtcagga atcacaaaaa aaacacaacc ctttaatgat
    10861 tttgtctatg ttttagtcat gctgaaaaat cacacaatga ccgtaaaata aaaccgtcac
    10921 tttcccacta actctggcgt ggacagaatc atcccgtatg attcccaagt ttggtaaata
    10981 ttattaaatc aggatgtgca agctatgatt cagtcatact aggaaaaaag tttttttgca
    11041 gattcgattg gtatcaatga agacgatata taataatatg caggatagga aaagttcagc
    11101 atccttttag atgttataca aaatccacct gctacgcagg tgaattattt actatgc
//
LOCUS       DAZGCQ010000142        11110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000142 DAZGCQ010000000
VERSION     DAZGCQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.142"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..428
                     /locus_tag="ACHK07_004454"
     CDS             <1..428
                     /locus_tag="ACHK07_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8104702.1"
                     /translation="FHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIY
                     NMNAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEIL
                     LLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            639..962
                     /gene="ybjQ"
                     /locus_tag="ACHK07_004455"
     CDS             639..962
                     /gene="ybjQ"
                     /locus_tag="ACHK07_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO8104703.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            959..1789
                     /gene="amiD"
                     /locus_tag="ACHK07_004456"
     CDS             959..1789
                     /gene="amiD"
                     /locus_tag="ACHK07_004456"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO8104704.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     STWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMRPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1786..2799)
                     /gene="ybjS"
                     /locus_tag="ACHK07_004457"
     CDS             complement(1786..2799)
                     /gene="ybjS"
                     /locus_tag="ACHK07_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8104705.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2898..4328)
                     /gene="ybjT"
                     /locus_tag="ACHK07_004458"
     CDS             complement(2898..4328)
                     /gene="ybjT"
                     /locus_tag="ACHK07_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO8104706.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4339..5340)
                     /gene="ltaE"
                     /locus_tag="ACHK07_004459"
     CDS             complement(4339..5340)
                     /gene="ltaE"
                     /locus_tag="ACHK07_004459"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO8104707.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLNKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5377..7095)
                     /gene="poxB"
                     /locus_tag="ACHK07_004460"
     CDS             complement(5377..7095)
                     /gene="poxB"
                     /locus_tag="ACHK07_004460"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO8104708.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7228..8196)
                     /gene="hcr"
                     /locus_tag="ACHK07_004461"
     CDS             complement(7228..8196)
                     /gene="hcr"
                     /locus_tag="ACHK07_004461"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO8104709.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFITGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8208..9860)
                     /gene="hcp"
                     /locus_tag="ACHK07_004462"
                     /gene_synonym="priS"
     CDS             complement(8208..9860)
                     /gene="hcp"
                     /locus_tag="ACHK07_004462"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO8104710.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10004..10903)
                     /gene="lysO"
                     /locus_tag="ACHK07_004463"
     CDS             complement(10004..10903)
                     /gene="lysO"
                     /locus_tag="ACHK07_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO8104711.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2710 a         2919 c         2932 g         2549 t
ORIGIN      
        1 tttttcatga ctgtagtatg catggtgtca agataaaacc atggttgcca gtaaaatgga
       61 caaaagagtt aataaatgat tatttgtatg gctgtttact ctctctgtac tctatctgtg
      121 caagagatat atataatatg aacgccggga ataacgtgaa ggttgctgcg gatgcttttc
      181 ttgaaatact ttattctctt aaaaataaat attgtattaa actattaagc gctcaggatc
      241 gggcgtttat atacgaattt gcaagaatga tcttcgctta tattaatgac aaaagcattg
      301 agatcttatt actttcttgt tttgcggcgg cggaccaaaa agcaatccag cggtatatac
      361 ctcaggcgca agatggggaa gactttagga atcatttaca atataagttg actcttccca
      421 ctcattaatg ttgccggcac aacataatag ggcaggtaag cggtgctcca taatgaaacc
      481 aatatccggt tacgaaacag ataattgtac aggcttatgt agctgttttt gacacaatga
      541 gatgatttcc taaactgatc aaaaccttct ttccaacatg gcatatcagt ccgataatgc
      601 tttagagatc gaaggcttaa caaacaagga gatcgatcat gcaattttcc acaactccaa
      661 ctctggaagg ccagaccatc gttgaatatt gcggtgtggt gaccggcgaa gcgattttag
      721 gtgccaatat tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgttccg
      781 gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc ctttgaggaa ttaggctccc
      841 aggcgcgggc gctgggggcc gatgccgtag ttggtattga tatcgactac gaaacggtcg
      901 ggcaaaacgg cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat
      961 gagaagagtc ttctggctgg tcgctgccgc tctgttactg gcagggtgtg caggcgaaaa
     1021 aggcattgtc gagaaagagg gataccagct tgatacccga cgccaggcgc aggcggcgta
     1081 tccgcgcatt aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc
     1141 gacactgacc gataagcagg tcagctcgca ttatctggtc cctgcggtac caccgcgata
     1201 caacggtaaa ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg
     1261 gattagcacc tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga
     1321 aaaccgtggc tggcaaaaat cggccggagt gaaatatttt gccccgtttg aaccggcaca
     1381 gattcaggcg cttattccac tggcgaaaga tattattgcc cgttatcaca tcaagccgga
     1441 aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa gacgatccgg ggccattatt
     1501 tccctggcag caactggcgc agcaggggat tggtgcctgg ccggatgcgc agcgggtgaa
     1561 cttttacctt gccgggcgcg cgccgcacac accggtagat accgcgtcat tgctggagct
     1621 tttggcgcgc tacggttatg acgttaaacc tgatatgaga ccgcgcgagc agcggcgcgt
     1681 gattatggcg ttccagatgc atttccgccc gacgttatac aacggcgaag cggatgcaga
     1741 aactcaggca attgccgaag cgttgctgga gaaatacggg caggattagc gcggcagttt
     1801 tccgtggtcg cgtagccagg cggcagtttt ctcgatacct tcatccaggg tgatgaccgg
     1861 ctgataacct aattcttcct gtgcacgcgt aatatccagc gtaaagtcaa aattaagctt
     1921 cgagacgccg tagtgggtca gcggcggctc ttttgctgac ttgcggccta aacgctccat
     1981 gctgcgggcg atcatatcca gcatcgggta ggggacggaa cgaatacgac agtcaatatt
     2041 caactcgtcg atcagcttct gcacgatgct gcgcagtgtg cgatgctcgc cgttggtaat
     2101 gttgtacaca cgcccggaag gtagcttatc gcaggcttcc tggcttgcca gccacatggc
     2161 gtgcactgca ttttcatagt aggtcatatc gaccagcgca cttccgccgt gcggtaacag
     2221 aatgctgccg tagtggtgca tcatatgcgc aagacggggg ataaagactt tatcgtgcgg
     2281 tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt tgtggattcg cctgcgaaag
     2341 catattgatc acttcttcgc tggctgcttt gctgcgggca aactcgttgg cgaagcggtg
     2401 agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa tcgaagtaca gggagggaga
     2461 agagatatga ataaagttac gcacacccca ggcgacagcc cattcaccca ggcggcgagt
     2521 ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc cagggagagg taaagctgga
     2581 gcagtgccac agcgtatcaa tgcccgcgag catcacttta gcttgtgatg agaccagctc
     2641 ggtcagatcc gccggaacaa actctgcgcc cattttttcc agcaatttac ccattgcctc
     2701 gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat aaaaactcta ccgcgtttcg
     2761 acctaagccg ctggtggcgc cggtaaccag taccttcata tcaatccact gttgttgaga
     2821 aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc aatgggaaac atgaaagaat
     2881 aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca gtctggcgat ttgttttgcc
     2941 attccgcgaa aaataaacag atgcgcgggg atcatcaata accagtaaaa cagccccggc
     3001 ataccgtgcg gatgccagaa agcgcggaca tcgatagtac gatagtcgcc tttatcttcc
     3061 agggtaaaac acagtcgtcc cagccccggt gctttcatgc caaataacaa cgaaagttgc
     3121 tttttcggtt caacgacaat gactttccag ctatccaccg catcgccagt ttgcaaatat
     3181 tcgcgctccg gacggccttt cgctaattta tgaccgatcg cacggtccat caacgcccgt
     3241 gtctgccaca aaatattgcc aaagaaataa cgctctttac cgccgatttg gttcactacc
     3301 tgccataaag ccgcaaggct ggcggacgtt ttaacggtaa accccgcctg tttggcgaaa
     3361 taaccgtact caggtcgcca gcgggcaaag gcctgagcgt cgtagcccca gtcgctggag
     3421 ttgaccagtt tttcttcctc tttcaacgtg ctacgtaccg cgtcatcgaa agcgatcagc
     3481 cgttgtggga tgagtgcacg tagcgcggtg tcatccgcca gcagatcgtg tttcagcccc
     3541 tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa tcacattaag aaaccacacc
     3601 gaaatccagc gagtggggag ggggatgggg atcaaccagc ggcgcttacc gctcaccgcc
     3661 ataaaatgtt caaactgttg ctgataactg agcacctctg gtccggcggc ttcgaagatg
     3721 cggtgttcgc tggccggatg atctaacagc gccaccagat agtgcagcaa gttttccagc
     3781 gcgatgggcg tggtgcgtga acgaacccag cgtggcggcg ttaacactgg cagattgtag
     3841 accatatcgc gcatgacttc gaacgctgct gaacctgcgc caacgataat tccggcccgc
     3901 agttcggtca ccgggacacc tgcttcacga agaatatccg ccgtagcctg acgagcacgc
     3961 agatgatccg actgctcatg tggcggggcc tgtaacgaac tgagaaagat taattgctta
     4021 actggtactt cacgtagcgc atcgcggacg ttgagagcca cctggcgctc ctgagcgata
     4081 aaatcgccgc tttcgcccat gctgtgcacc agaaaataga cggtatcgat atcctgtaac
     4141 agggccggaa ggttatccgg ccagctgaga tcgactttat ggcaactgac gtttgccagt
     4201 tgcagctttg caagcctgtc gacatgacgt gccgccgcca ggatctgatg cccttgctgg
     4261 ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt
     4321 tgcggcacgt ttctctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc
     4381 cagctgttcg cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat
     4441 cagcacgttt ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg
     4501 aacaaacagc atattagtgt cctgacgcat cacatccgcg cctgcttcac gcagctgctc
     4561 cgccatccag gcagcgttgt cgtggtcttc ctgcaagcgc gcaacattat ttttcagggc
     4621 atatatcccg gcggcagcca gaatgccgga ctggcgcatc ccgccacctg tcattttccg
     4681 ccagcgaatg gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt
     4741 cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa tattgcgtga tctctttcag
     4801 ttcgcagccg taagccacca cggcattaaa gatgcgcgca ccgtcaacat gcagcgccag
     4861 attgcgctcg cgggtaaatt cccatgcatc tttaaggtat tcacgcggca acactttgcc
     4921 gttgtgggtg ttttccagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt
     4981 gattttcatc gccactttat tcagcggtag cgtgccgtcg gcagccgcgt cgatgggttg
     5041 cggctgaata ctgcccagca ccgctgcgcc accggcttca aacagatagt tatgcgcggc
     5101 ctgaccgaca atatactctt cgccgcgttc gcagtgactg agcagagcga ccaggttggc
     5161 ctgagtgccg gtaggcagaa aaatggcggc ttctttaccg gaaagctctg ctgcatagtc
     5221 ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat
     5281 catcgcttcg agcatggcgc ggctcggtcg ggttacggta tcactgcgta aatcaatcat
     5341 ggcatgtcct tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc
     5401 cagttcgatc acttcatcgc cgcgtccgct gatgattgcg cgcagcatat acaggctgaa
     5461 acctttggcc tgttcgagtt tgatctgcgg tggaatggct aactcttctt tggcgaccac
     5521 cacatccacc aataccggac cgtcgatgga gaaggcgcgt tgcagggctt catcaacttc
     5581 agacgctttt tctacacgga tacccgtaat gccgcatgct tcggcaatgc gggcaaagtt
     5641 ggtgtcgtgc agctcagtac cgtctgtcag gtagcctccg gctttcatct ccatcgccac
     5701 aaagcccagc acgctgttat taaagacgat aattttcacc ggcagtttca tctgcactac
     5761 cgagaggaaa tcgcccatca acatgctaaa accgccatcg ccgcacatgg cgaccacctg
     5821 acgttccggc tcggttgcct gcgcgcccag cgcctgcggc atggcgttag ccatcgaacc
     5881 gtggttaaac gaacctaaca ggcgacgctt gccgttcatt ttcagataac gcgctgccca
     5941 caccgttggc gtaccaacgt cacaggtgaa aatggcgtca tcggcggcaa aatgactaat
     6001 ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcgtccag
     6061 ccctttgcgg gcatcgcggt aatcttccag cgctttatcc agaaatttgc gatcggcttt
     6121 ttcttccacc agtggcagca atgcacgcag ggttgatttg atatcgccga ccagtgccat
     6181 atccaccttg ctgtgagcgc cgatgctggc tggattgata tcaatctgaa taattttggc
     6241 atcggtcggg tagaacgcac ggtaggggaa ctgagtaccg agtagcacta acgtgtcggc
     6301 gttcatcatg gtatggaaac ctgacgagaa tccgattaac ccggtcattc caacatcata
     6361 cggattatcg tattcgacat gctctttacc gcgtagggca tgaacaatag gtgctttaat
     6421 tttcccggca aactcaacta actctttatg cgcccccgcg cagccgctgc cacacatcag
     6481 agcgatattg ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg
     6541 cgtcacaact ggctgtggcg catgatacca gtgcgtggtt gccccttctg gcgcaggttt
     6601 taacgccacg tcacccggta acacgacaac ggaaacgcca cggttaagca ccgctttgcg
     6661 catagcaatt gccagcactt gtgggatctg ctccgggctg gaaaccagct cgcaatagtg
     6721 actacattcg cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgcctatttc
     6781 gctggaggga atatgagcgg caatcgccag taccggaacg tgattgcggt ggcaatcgaa
     6841 caggccgttg attaagtgca ggttgccggg gccgcacgat ccggcacaga ccgccagttc
     6901 tcctgaaagt tgtgcttcag cgccagcggc aaacgccgcc acttcttcat ggcgggtgga
     6961 catccactcg atggtgccca tgcgattaag actgtcgcta agaccgttca gagagtcgcc
     7021 cgtgactccc cagatgcgtt tcacgcctgc cgattcgagt gttttggcga tataagctgc
     7081 aaccgtttgt ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca
     7141 tctgacggag ggggaaggga tgggagaaaa aggaggcact aacggttaaa tagcccgatg
     7201 aaatgaatat catcgggcat aaggcgatta tgcgagaacc aaatccccct gcggatggca
     7261 ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtgctgct
     7321 caccgtatat tcaccggaaa ccacttttgt cttacagcag ccgcaaacac ccgcacggca
     7381 ggcagcgaca accggaacgt tattgctttc cagcgcctcc agtagcgtag tgccaaccgg
     7441 ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc
     7501 cgctactggg gtgaagaatt tctctttaaa gaaacgcgtc acgccgagcg ctttcacttc
     7561 ctgctctacc caatccatat acggtgctgg accacaagtc atcacggtac gtgaagctaa
     7621 gtcaggaacg cgagtcagga gttcgcgagt gagacgacca gtgataaagc cttcggtaac
     7681 gttattttcc gccaccagcg ttaccggata gttacgccac tcatcggcga aaataacatc
     7741 ctgcggcgta cgcacgttgt agatcacctg tacatcggcc tgtggacgat tcttcgccag
     7801 ccagcgacgc atcgacataa tcggcgtgac gccgcagcct gcagccagca acaggaattt
     7861 atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc
     7921 accgcgtttt acatcgcggg tcagccactg ggagccgaca ccgtcatcaa tccgccgcac
     7981 ggtcagggtg atatattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt
     8041 ttccgctgag ttacgcacgc tgaccagtgc atattgcccg gcgcggtatg ggtagtaatc
     8101 gtggcaaatc agggaaatcg tccacacatc cggcgtttct tgcttaatgt gatgaacctg
     8161 catccgccac gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgctcaac
     8221 agttgcttca tgtcttcttc aacagtggtg atagaacgca ggccgaattt ctcgttcagc
     8281 accgccagca gatctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc
     8341 acgcccagag aaagcagcgt cagcagaatg acgatcgctt tctgttcaaa ccaggagagc
     8401 accagcgaca gcggcagatc gttgacaccg cagcccagtt tctctgccag cgtaaccgcc
     8461 agaataatcg ctgagtaagc atcgttacac tgaccggcat ctaccagacg cggcagacct
     8521 tcgatatcgc caaactcaag tttgttaaag cgatatttac cacaggcgag ggtcaggatc
     8581 aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt agtggcgctc gccgcgtgcg
     8641 ccgtcacagc caccaagcag gaagatatga cgcagttttt cacggctcac cagatcaatc
     8701 agcgtatccg ctgcgccaag cagcgtctgg cggccaaagc ccacggtgat caggtgcgga
     8761 atttcgctgt acgggaagcc cgccatttgt tgcgcctggg caatgaccgc agagaaatct
     8821 tcgccatcca gatgacgcac acccggccag ccaacgatgc tgcgggtcca gatacgatcg
     8881 tcataagcgc ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggccaggg
     8941 aaacgagcga actccacttg ctgattctgc cagccgctgc cgtagttacc gaccagatgc
     9001 ttgaatttac gcagctccgg gtagccatgc gcaggcagca tttcgccgtg ggtgtagaca
     9061 ttaacacccg tgccttcggt ctgttccaga aggttgtaga gatctttgag atcgtgaccg
     9121 gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga cctgggttgg cgtcgggtga
     9181 ccgtatttac cggtttcgcc tgcatccaga atgctcatca ctttgaagtt catctggccg
     9241 atttccattg aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc
     9301 atgattttat ggtactgggc ataaatatca ttgtcgtatt gaccgagaac gtgtgcgtgt
     9361 tccatatagg ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg
     9421 ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt ctgctgcctg acgttgcagt
     9481 tcgccgagat cgtcgctcac cagttgcagg tcagccatcg ggttatcgac gcgggcgttg
     9541 gcatctacag ccaggcattg tgctttcagc gcctcgcgca aggcaatcgc ttcacgagcg
     9601 tagccgacaa tacgcggaga atcgaagtta acgttggtca gggttgagaa aaatgcgcgt
     9661 ggcgcaaagc tgtctacatc gtggttgatg atgccgtatt cacgcgcttt taccgcccag
     9721 gccgaaagcc cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaggt ttccgccgtt
     9781 ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg ccggagtacg gatagtttgt
     9841 tcacattgca cacaaaacat gatcacacct tttaaagtta tatttaatat acatctttaa
     9901 ggttaagacg cttaacgcgg ggataaaagg gatttttcat gcaactttaa gggagattga
     9961 tttagcgcaa ttttggcggc agggatctac cgccagagag gtattacgca gagaaaaagg
    10021 cgatgaggat cggcactaac aggctaagaa taaaaccgtg aacaattgcc gccgggacca
    10081 tatccagccc gccagtacgt tgaagaacgg gcagggtgaa atccatcgac gtcgcaccgc
    10141 ataagcccag tgcggtagag cggctgcggc gaatcagccc agggatcaac ataattgcaa
    10201 tcagctcacg ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt
    10261 cggtcaataa aataccggaa agagaatacc agccgaaacc agaggccatt gccagcgcgg
    10321 tattgatggg tagatcaaga ataaaggcgt taattaaacc accaattaat gaactggcaa
    10381 ccaccaccac ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc
    10441 cattattgcg caactgaata ccgacgagga aaagtagcag aattaaggtg tattcactgg
    10501 cttcggtcgc gtgttgtaag aaagccagtc cactcaggcc aatggcaaaa ccaatcacta
    10561 ctacgccgca cagttttagc gactcaagcg ccatcgcaat acgcgacggg agtttttctt
    10621 gctgatggtg gttgcgccac ggcagaccgc gctccagcca cataagggcg gcaatattac
    10681 acagtaaaat aacggtaata ctgacggcag aataatgcag aatcgccaac aggttactgg
    10741 cgaggttatc gagaaacgcc agactgatac ccataaaaaa gagaataagg taaaccatcc
    10801 agcttagtag ctgattaata acttttaacg cagcttgttg gcgaagcgga atgaggtaac
    10861 ccacaatcag gggaaccaga atgattaaca acccagaaaa catgaaaacc cagcccttgc
    10921 aaagaaagaa gtcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt
    10981 gcaattaaaa ggaaatgtta tgcataagga tcggtagggt attcattttc atttaaagat
    11041 attcttgctc tttaattaca aaccgcaccg gtgttggtgg cgtcaaaatc gccgaggcgt
    11101 tccctgaagg
//
LOCUS       DAZGCQ010000143        11018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000143 DAZGCQ010000000
VERSION     DAZGCQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.143"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(175..1839)
                     /gene="glnS"
                     /locus_tag="ACHK07_004464"
     CDS             complement(175..1839)
                     /gene="glnS"
                     /locus_tag="ACHK07_004464"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO8104712.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2042..3988)
                     /gene="nagE"
                     /locus_tag="ACHK07_004465"
     CDS             complement(2042..3988)
                     /gene="nagE"
                     /locus_tag="ACHK07_004465"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO8104713.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATTAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4321..5121
                     /gene="nagB"
                     /locus_tag="ACHK07_004466"
     CDS             4321..5121
                     /gene="nagB"
                     /locus_tag="ACHK07_004466"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8104714.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5181..6329
                     /gene="nagA"
                     /locus_tag="ACHK07_004467"
     CDS             5181..6329
                     /gene="nagA"
                     /locus_tag="ACHK07_004467"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8104715.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6338..7558
                     /gene="nagC"
                     /locus_tag="ACHK07_004468"
                     /gene_synonym="nagR"
     CDS             6338..7558
                     /gene="nagC"
                     /locus_tag="ACHK07_004468"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO8104716.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7606..8358
                     /gene="nagD"
                     /locus_tag="ACHK07_004469"
     CDS             7606..8358
                     /gene="nagD"
                     /locus_tag="ACHK07_004469"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO8104717.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8409..8507)
                     /locus_tag="ACHK07_004470"
                     /pseudo
     CDS             complement(8409..8507)
                     /locus_tag="ACHK07_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8755..10419
                     /gene="asnB"
                     /locus_tag="ACHK07_004471"
     CDS             8755..10419
                     /gene="asnB"
                     /locus_tag="ACHK07_004471"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO8104718.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10799..10875
                     /locus_tag="ACHK07_004472"
     tRNA            10799..10875
                     /locus_tag="ACHK07_004472"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10833..10835,aa:Met,seq:cat)
     gene            10885..10969
                     /locus_tag="ACHK07_004473"
     tRNA            10885..10969
                     /locus_tag="ACHK07_004473"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10919..10921,aa:Leu,seq:tag)
BASE COUNT         2743 a         2861 c         2801 g         2613 t
ORIGIN      
        1 ggatgcggcg tgaacgcctt atccggccta caaaagcacg caaactcaat atattgcaga
       61 gatcatgtag gcctgataag cgtagcgcat caggcaattt agcgtttgaa actgtttgtc
      121 tgttccttgc cggatgcgac gtaaacgccc catccggcat agcgaaactt aaaattactc
      181 gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttccgc
      241 cgtagaatgg cggctatcga ggcagaagta accttcacgc tcaaactgga atgctttacc
      301 cgctaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg
      361 gttaatcacc gacaggaaat catccgcagc acctgggtta ggcacgctga acagacgatc
      421 atacaaacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacgccttt
      481 gactttacga ccatcagccg gatctttgct taaggtgtcg gcgtcataag tacagaagat
      541 ggtggtgata ttaccttcgg catctttctc gacgcgttct gccttaatca cataagcatt
      601 acgcagacgc acttctttac cgagcaccag acgtttgtac tgcttgttag cttcttcgcg
      661 gaaatcggcg cgatcgatcc aaatctcacc gctaaacggc acctgacggc tgcccatttc
      721 cggtttgttc ggatggttcg gcatggtaac catttcgcct tcgccctgat agttttcgat
      781 aaccagtttc accggatcga taaccgccat tgcgcgcggc gcattttcgt tgagatcttc
      841 acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg tcacgccgat
      901 gcgtttgcag aactcacgaa tagaagccgc agtgtaacca cgacgacgca gaccggaaat
      961 ggtcggcata cgcgggtcat cccagccttc aacgtgctta tcggtcacca gcaggttcag
     1021 cttacgcttg gacatcacag tgtattccag attcaggcgc gagaactcat actggcgcgg
     1081 gtgaacagga atggtgatgt tgtccagtac ccagtcgtac agacgacggt tgtcctggaa
     1141 ctccagcgta cacagagagt gcgtaatacc ttccagcgca tcgctgatgc aatgggtgaa
     1201 gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag caaatttaat
     1261 gcggtacagc accggatcgc gcatcacgat aaacggtgaa gccatgtcga ttttcgcacg
     1321 caggcaggct ttaccttctt caaaaccgcc ggtacgcatt ttctcgaaca gcgccaggtt
     1381 ctcttcaacg ctacggtcgc ggtacgggct gtttttacca ggctgcgtca gggtgccgcg
     1441 gtattcgcgg atctgttccg gcgtcagttc atcaacgtac gccaggcctt tattgatcag
     1501 ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga
     1561 ccagtgaaaa cctaaccact caacgtcgtt tttgatcgaa tcgacatact cgatatcttc
     1621 ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat
     1681 cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg
     1741 cgggaaacgg gtgtgtactg tggtgtgctt accactggcc agatcttcat cgatgatctg
     1801 acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa
     1861 acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt tagttagtga
     1921 aaatcgaaaa tctcactgag attatgggga tgattgatga aaaaaaaagc ggcagagaag
     1981 atctccaccg cttgtcattg ttggatgcga cgctcaagcg tcgcatcagg cataaagcag
     2041 attacttttt gatttcatac agcggtgttt gacccgccac aacatggccc tgagctttaa
     2101 tgatcaagcc actgaaatcg tcgatattac tgcaaaccac tgggctaatc atcgagcggg
     2161 cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgtcctgcg cttacctgcg
     2221 cgccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg tcgataccca
     2281 tatggacgac gatctccgcg cctttttcgg tttccagaca gaacgcgtgg ttggtgttga
     2341 agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc ggtttcaccg
     2401 ccacaccgtc acccaccgct ttgctggcga atgcttcgtc aggaacctga tccagtgcca
     2461 cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt ggtacagcct
     2521 gcggttttgc tacaggagcg gtagttgccg gagcagtttc agcagaggca gctgctaccg
     2581 gaccgcgggc aacgactttc ttcatcgcat cgccgatgga ttctgctttc gcgccaacaa
     2641 tcacctgaat agtctgtttg ttcagtttta ctaccccaga agcccccaga cgtttacaca
     2701 tcgtatcgtt aacgcgggca gagtcagcca cggtaaggcg cagacgggtg atacaggcgt
     2761 caattgcttt caggttgtca gtgccgccaa ccgcagcaat atagttggtc gccagttgat
     2821 tgaggccttc ttcagtgttg ctgttggctt cttcagtaac gatctcgtct tctttatctt
     2881 cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc acgaagtaga
     2941 tagcgaagaa gacaacaccc ataaccagca gcatccagac gttctggctg gcggccggca
     3001 ggttatacat caacgcgtag tcgatagccc ccgcagagaa agagaagccg gcatggatac
     3061 ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg aggtacagca
     3121 gcggagcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc aggaacgcag
     3181 taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc ggtgctgcga
     3241 agtacatcgc cagcgccgca cccggcaggc cgaacatcat gatcgggaag aagccggaca
     3301 tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg tggaaaaccg
     3361 tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc agcacctgat
     3421 gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa cccagcgcgc
     3481 ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc ggccagacgt
     3541 aaccaaaaat ggccgccagc accagacaga agaagccggt ggcgatcggc acaaagcgtt
     3601 tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg ttataggctg
     3661 cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt tctgggttga
     3721 tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc gccgccgcac
     3781 ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat gcgaggttat
     3841 caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct ggctgaccga
     3901 atcgcagcaa cagtgccgcc accggcagca ccgcgatagg gagctgtaac gccctaccga
     3961 gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc tatttggctc
     4021 gtttaaagcc gtatttttat tgtgctgcaa attgtactgc cgatgttctg taatcagatt
     4081 gttagatcat ctgccacaga gtgtgtgaaa atttaattcg tatcgcaaat taaacgcgtg
     4141 tcttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact ggctaaacca
     4201 gaaaacttat tttatcattc aaaaaatcag gtcggattga cgcctgtctg cgcaaatcca
     4261 ggttacgctt aaagatgcct aatccgccaa cggcttacat tttacttatt gaggtgaata
     4321 atgagactga tccccctgac taccgctgaa caggtcggca aatgggctgc tcgccatatc
     4381 gtcaatcgta tcaatgcgtt caaaccgact gctgatcgtc cgtttgttct gggcctgcca
     4441 actggcggca cgccaatgac cacctataaa gcgttagtcg aaatgcataa agcaggccag
     4501 gtcagcttta agcacgttgt caccttcaac atggacgagt atgtcggtct gccgaaagag
     4561 catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt tgatattcca
     4621 gcagaaaaca tcaaccttct caacggcaac gccccggata tcgacgccga gtgccgccag
     4681 tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac
     4741 gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa
     4801 accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag
     4861 gtgccaaaat atgccctgac tgtcggtgtt ggtacactgc tggatgccga agaagtgatg
     4921 attctggtgc tgggtagcca gaaagcactg gcgctgcagg ccgcagttga aggttgcgtg
     4981 aaccatatgt ggaccatcag ctgtctgcaa ctgcatccga aagccatcat ggtgtgcgat
     5041 gagccttcca ccatggagct gaaagttaag actttaagat atttcaatga attagaagca
     5101 gaaaatatca aaggtctgta attgttatcc ctgccctgac accttgctca gggcaatatt
     5161 ttttaaaatc gggggtcaga atgtatgcat taacccaggg ccggatcttt accggccacg
     5221 aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc gtctgtccgg
     5281 tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt ctctcccccg
     5341 gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac accgctgaag
     5401 cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca ggctgtacta
     5461 actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc gtgcgcgtta
     5521 tgcgcgagta cctggcaaaa catccgaatc aggcgttagg tctgcatctg gaaggcccgt
     5581 ggctgaatct ggtgaaaaaa ggtacccata atccgaattt tgtgcgtaag cctgatgccg
     5641 cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc ctggcaccgg
     5701 aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg gtttctgccg
     5761 gtcactccaa cgcgacgttg aaagaagcga aagccggttt ccgcgcgggg attacctttg
     5821 ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg
     5881 cgatcctcga tgaagctgac atttattgcg gtattatcgc tgatggcctg catgttgatt
     5941 acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg gttaccgacg
     6001 ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa acaatatact
     6061 accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc ttaaccatga
     6121 ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta
     6181 tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa acgtctcggc acactcgccg
     6241 caggtaaagt agccaacctg accgcattca cacctgattt taaaatcacc aagaccatcg
     6301 ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg gacaagctca
     6361 gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc gcctgattga
     6421 ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg cccccgccag
     6481 cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag ttgatcagca
     6541 ggcctccacc gggggccgcc gcgctatctc tatcgtcacc gaaacccgca atttccacgc
     6601 aatcggcgta cggcttggtc gtcacgacgc caccatcacc ctgtttgatc tcagcagcaa
     6661 agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcagacgc tggaacatgc
     6721 cctgctgaat gccattgctc agtttattga tagctaccag cgcaaactgc gcgagctgat
     6781 cgccatttcc gttatcctgc caggacttgt tgacccggac agcggcaaaa ttcattacat
     6841 gccgcatatt caggtagaaa actgggggct ggtagaagca ctggaagagc gttttaaagt
     6901 gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact acttcggtgc
     6961 aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg gggccgggat
     7021 tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa ttggccatat
     7081 tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc tggaaactat
     7141 cgctgccaac gccgctattg aacaacgggt gttgaatctg ttaaagcagg gctaccagag
     7201 ccgcgtgccg ctggacgact gcaccataaa aactatctgc aaagctgcga acaaaggcga
     7261 tagcctggcg tcggaagtga ttgagtatgt cggtcgtcat ctgggcaaaa ccatcgccat
     7321 tgccatcaac ctgtttaacc cgcaaaaaat tgttattgcc ggtgaaatca ccgaagccga
     7381 taaagtgctg ctccctgcta ttgaaagctg cattaatact caggcgctga aggcgtttcg
     7441 cactaatctg ccggtggtac gttctgagct agaccaccgc tcggcaatcg gcgcttttgc
     7501 gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg aaaattaatg
     7561 tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac cattaaaaat
     7621 gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg
     7681 gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac caactatcct
     7741 tcgcagaccg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga tgtacctgac
     7801 agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca ggaaggcaag
     7861 aaagcgtatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc cggtttcact
     7921 attaccgatg tgaaccctga ttttgtcatt gttggcgaaa cgcgttccta caactgggac
     7981 atgatgcata aagcggccta tttcgtcgct aacggtgcac gctttatcgc caccaacccg
     8041 gatactcacg ggcgcggttt ttatccggcc tgtggcgcgt tgtgtgcagg gattgagaaa
     8101 atctccgggc gcaaaccgtt ctatgttggt aagccgagtc cgtggattat ccgcgcggca
     8161 ttaaacaaaa tgcaggcaca ttcggaagaa acggtgattg tcggcgataa cctgcgtacc
     8221 gatattctgg ccggcttcca ggcaggtctg gagacgattc tggtgctttc tggtgtttcg
     8281 tcgctcgacg atatcgacag tatgccgttc cgccccagct ggatttaccc atcggtcgct
     8341 gaaatcgacg ttatctgaat agcaaccacg tctgcggacg tggttggctc tgatgacaaa
     8401 cgccaaactg cctgatgcgc tacgcttatc aggcctacgt agctcctgca acatattgaa
     8461 ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaatcgc
     8521 actttgtcag caatttggcc cccgtaaaat ataaacattc aataaaaccc gccaaaaatt
     8581 atcgatccat taataaatac atcaccgcac cctccccttt ttcacaaatc agcaatcacc
     8641 attacgtttt ttattttttc accgccaaat cgcttgcgca cagtgctgct ttgcggcatt
     8701 tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa ggttatgtgt
     8761 tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa gaaagcactc
     8821 gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta tgccagtgat
     8881 aacgccattc tcgcccacga acgtctgtca attgttgacg ttaacgcggg ggcgcaacct
     8941 ctctacaacc aacaaaaaac ccacgtactg gcggtaaacg gtgaaatcta caatcaccag
     9001 gcattgcgcg ctgaatacgg cgatcgttat cagttccaga cagggtctga ctgtgaagtg
     9061 atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca gggcatgttt
     9121 gcctttgccc tgtacgacag cgaaaaagat gcctacctga ttggtcgcga ccatctgggg
     9181 atcatcccac tgtatatggg ctatgacgaa catggtcagc tgtatgtggc ctcagaaatg
     9241 aaagccctcg tgccggtatg ccgcacgatt aaagagttcc cggcggggag ctatttgtgg
     9301 agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgacta cgatgcggtg
     9361 aaagataatg tcaccgacaa aaacgagctg cgtcaggctc tggaagattc cgttaaaagc
     9421 catctgatgt ctgatgtgcc ttacggtgtg ctgctttctg gtggtctgga ttcctcaatt
     9481 atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga acgctctgaa
     9541 gcctggtggc cgcagttaca ctcctttgct gtaggtctgc cgggttcacc ggatctgaaa
     9601 gcagcccagg aagtggcaaa ccatctgggc actgtgcatc atgaaattca cttcactgta
     9661 caggaaggtc tggacgccat ccgcgacgtg atttaccaca tcgaaaccta tgatgtgacc
     9721 actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaagc gatgggcatt
     9781 aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct ttacttccac
     9841 aaagcaccga atgccaaaga actgcatgaa gagacggtgc gtaaactgct ggccctgcat
     9901 atgtatgact gcgcgcgtgc caacaaagcg atgtcagcct ggggcgtgga agcacgcgtt
     9961 ccgttcctcg acaagaaatt cctcgacgtg gcgatgcgca ttaacccgca ggataaaatg
    10021 tgcggtaacg gcaagatgga aaaacacatc ctgcgtgaat gttttgagtc atacctgccc
    10081 gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta cagttggatc
    10141 gacaccctga aagaagtggc ggcgcagcag gtttctgatc aacagctgga aactgcccgc
    10201 ttccgcttcc cgtacaacac accgacctca aaagaagcgt atctgtaccg ggaaatcttt
    10261 gaagaactgt tcccgcttcc aagcgccgct gagtgcgtgc cgggcggtcc ttccgtcgcg
    10321 tgttcttcag ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga cgatccgtct
    10381 ggtcgcgcgg ttggtgttca ccagtcggcg tataagtaag aaatgcgaaa cagccccggt
    10441 gaaatccggg gctgtttcag ttattgaggt gcttgttgat tctgatatgc agtaaggaaa
    10501 tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag tatttcatag
    10561 attaatttcc caccactatt gttaaattcc gaatttcatc ctgtatgaca aaagaatggc
    10621 tgtagggtgg gaaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc
    10681 gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca
    10741 aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaaagatgg
    10801 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    10861 ccgtcgtagc caccatcttt ttttgcggga gtggcgaaat tggtagacgc accagattta
    10921 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    10981 gcgttgtacg gatggggtat cgccaagcgg taaggcac
//
LOCUS       DAZGCQ010000144        10917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000144 DAZGCQ010000000
VERSION     DAZGCQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.144"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /gene="ecpR"
                     /locus_tag="ACHK07_004474"
                     /gene_synonym="matA"
     CDS             <1..285
                     /gene="ecpR"
                     /locus_tag="ACHK07_004474"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO8104719.1"
                     /translation="NYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKIT
                     DREMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            311..946
                     /gene="ecpA"
                     /locus_tag="ACHK07_004475"
                     /gene_synonym="matB"
     CDS             311..946
                     /gene="ecpA"
                     /locus_tag="ACHK07_004475"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO8104720.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSKLVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1004..1672
                     /gene="ecpB"
                     /locus_tag="ACHK07_004476"
                     /gene_synonym="yagY"
     CDS             1004..1672
                     /gene="ecpB"
                     /locus_tag="ACHK07_004476"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO8104721.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1698..4223
                     /gene="ecpC"
                     /locus_tag="ACHK07_004477"
                     /gene_synonym="yagX"
     CDS             1698..4223
                     /gene="ecpC"
                     /locus_tag="ACHK07_004477"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO8104722.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSMWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4213..5856
                     /gene="ecpD"
                     /locus_tag="ACHK07_004478"
     CDS             4213..5856
                     /gene="ecpD"
                     /locus_tag="ACHK07_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO8104723.1"
                     /translation="MRVNLLITMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5825..6535
                     /gene="ecpE"
                     /locus_tag="ACHK07_004479"
     CDS             5825..6535
                     /gene="ecpE"
                     /locus_tag="ACHK07_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO8104724.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6848..7177
                     /locus_tag="ACHK07_004480"
     CDS             6848..7177
                     /locus_tag="ACHK07_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8104725.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7388..8002)
                     /locus_tag="ACHK07_004481"
     CDS             complement(7388..8002)
                     /locus_tag="ACHK07_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO8104726.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8049..8297)
                     /locus_tag="ACHK07_004482"
     CDS             complement(8049..8297)
                     /locus_tag="ACHK07_004482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104727.1"
                     /translation="MSPANKFMFAGMLMMMGAGLLLELYDAKTDKETCDCQLCITLQI
                     YVTSNIKIGRNDGAYILWRLDNHLVIQNNTVLYIYLVL"
     gene            8420..9109
                     /gene="paoA"
                     /locus_tag="ACHK07_004483"
     CDS             8420..9109
                     /gene="paoA"
                     /locus_tag="ACHK07_004483"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO8104728.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9106..10062
                     /gene="paoB"
                     /locus_tag="ACHK07_004484"
     CDS             9106..10062
                     /gene="paoB"
                     /locus_tag="ACHK07_004484"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO8104729.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPDAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10059..>10917
                     /gene="paoC"
                     /locus_tag="ACHK07_004485"
     CDS             10059..>10917
                     /gene="paoC"
                     /locus_tag="ACHK07_004485"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO8104730.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATL"
BASE COUNT         2773 a         2928 c         2845 g         2371 t
ORIGIN      
        1 aattattggt attacaacag caatattaga ggcgtggtat acgctggact gagtcgtgat
       61 attagaaaag aactggccta tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa
      121 gataaaatca ctgaccggga aatggaaatt atccgcatga cggcccaggg aatgcaacct
      181 aaatcgattg ccagaattga aaattgtagt gtgaagacag tgtataccca tcggcgtaat
      241 gctgaggcca agctgtactc aaaaatatat aagttggttc agtaaactcc aggcaagtta
      301 gttttaaaaa atgactcact gggacatcac gtcctcaatt caactcggga agaaatgcaa
      361 tgaaaaaaaa gttctggcaa tagctctggt aacggtgttt accggcatgg gtgtggcgca
      421 ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca aaaaagacac
      481 caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg ccgaaggcat
      541 taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc
      601 taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt tggatacctc
      661 aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga
      721 taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggctaa
      781 cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcaccttca ccatcatcag
      841 cggcaccacc aatggtacca ccgcagtaac cgactacagc actctaccgg aaggcatctg
      901 gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc tctgatgtac
      961 cagcaggggt agcccccctg ctttattcct ggacggaatt gttatgaaaa agcaccttct
     1021 gcctctcgct ctgctgtttt ccggaatatc tccggcccag gcgctggatg tcggcgatat
     1081 atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa acagtaccga
     1141 cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg acgacgggca
     1201 ggttatctca atggacaagc cggatgagtt gctactcact cccgccagct tgctgctacc
     1261 cgcccaagcc agcgaagtga tccgcttctt ctataaggga cccgcagatg aaaaagagcg
     1321 ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa
     1381 ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg tcgcccctcg
     1441 ccaggcgaac taccactttc agtacgccaa cggctccctg acaaatacag gaaatgcgac
     1501 gctgcggatc ctcgcctacg gcccttgcct gaaagccgcc aacggtaagg agtgtaaaga
     1561 gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca ctgcggataa
     1621 caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat agatagctgt
     1681 gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa gcccagtttg
     1741 ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc
     1801 agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag gacggcatga
     1861 gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat cagcgaatcg
     1921 gcagcgcttt tatctggctg gatgatggac agctacgcat ccggaaaata cagctggaag
     1981 agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg gctctggcga
     2041 acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag ctggatctca
     2101 gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc accgtactac
     2161 gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt aatctgagct
     2221 ataacttggg cgtctataac aaccagttgc gtaacggcgg gagcaacaca tccagctatc
     2281 tgtcgctgaa taacgttact gcgctgcgcg aacatcatgt ggtgctcgac ggctcgctgt
     2341 acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat gaacgcgatt
     2401 ttgccggtca ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag tccttagggc
     2461 cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac caggccagct
     2521 ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta ccggcggcgg
     2581 gtgaagtaca tctcacccgt gatgggcggc tactaagcgt tcagaacttc actatgggca
     2641 atcatgaagt ggatacccgg ggtctaccgt acggtattta cgatgtggaa gttgaggtga
     2701 tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt caataagctg tttagccggg
     2761 ggcgcggcgt tggtgcacca ctggcgtggc aggtatgggg cggtagcttt catatggatc
     2821 gctggtcgga aaacgggaaa aagacgcgac cagctaagga gagctggcta gcaggtgcct
     2881 cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga tacgataatc
     2941 aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg agcgatcaac gttaacctgc
     3001 aaaatatgct ggccagtgac agctcatgga gcaacatcgc cagcatcagc gccactctac
     3061 cgggaggctt tagttcgatg tgggttaacc aggaaaaaac ccgcattggc aatcaattgc
     3121 gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac tcactgtggt
     3181 cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac agccattatt
     3241 acacagcaga ttactatcaa aatgtctaca gcggtccctt tggttcgctt ggcctgcggg
     3301 ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa tatatcgctc
     3361 tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgactcat caaaacggct
     3421 acaccatggc aaacctgtca gcacgcaagc agtttgatga agggaccatt cgcactgttg
     3481 gtgccaatct gtctcgagcc atctccggcg ataccggtga tgacaaaact ctcagtggtg
     3541 gggcgtatgc acagttcgac gctcgctacg ccagcggaac gctgaacgtc aatagcgcgg
     3601 cggacggcta cgtcaatacc aatttaaccg ccaatggcag cgtcggctgg cagggtaaaa
     3661 acattgctgc cagcgggcgg actgatggca acgctggggt gatattcaac accgggctgg
     3721 aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccactt aacggcaagc
     3781 gtaactatct cccactctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa
     3841 actcactcga cagttacgat atcgtcagcg gccgcaaaag ccatctgact ctctatccag
     3901 gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc
     3961 gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc
     4021 gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc actatcgatt
     4081 ttcgctacag tggcaataaa acctgcgaag tggctctgga actcaaccag gcgcgcggcg
     4141 ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga
     4201 caggagaaga gaatgagagt taacctacta ataacgatga taatctttgc gctaatctgg
     4261 cctgcaaccg cgctcagagc tgcagtgagc aaaacaacct gggcggatgc accggcacgc
     4321 gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg
     4381 ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacaa tggttcagga
     4441 accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg cctctatacc
     4501 aactggaagt ttgatatgtg gctggaaaat tcgccggttt catctccttt aactggctta
     4561 cgctgcatca actggtacgc agggtgtaat atgaccacca gtcttatcct gccgcaaacc
     4621 accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac
     4681 ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt
     4741 acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag cggcgcacgc
     4801 tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac gaaagcagca
     4861 aatctacggt tgataaatac ccactcgctg gcggaagtat ttatcaacag cgacggagta
     4921 ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa ccatcggcag ccgttcagga
     4981 ttaagttgta agatggttaa ctataccctg caaacaaacg gactcagcaa cacctcaatc
     5041 catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg
     5101 cagttcagtc tgaatggcag ttcatggaaa ccggtgagca ataccgccta ttactacacc
     5161 ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa cttctttaag
     5221 cagatggtga acctcgggat cagcgatatc aacaccaaag atctattcaa ctttcgcttt
     5281 cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa cacgctgatt
     5341 atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca gacaccgtcg
     5401 cgggagggat atgttggcag cggcgagtcg gcactcgatt tcggctatat cgtaaccacc
     5461 agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt
     5521 ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt accgttcccg
     5581 gcgacacttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat
     5641 gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac tgatatctct
     5701 ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat ggataacgcc
     5761 atttcgctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc ttcaggagag
     5821 attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag cggtctgtat
     5881 gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc
     5941 cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcac ggatataccg
     6001 tattgccatc agtgctattg atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga
     6061 tggtgaactg cttttcgccc cccgccagct ggcgttgcag gctggtgaga gcgagtattt
     6121 taaattttac tatcatggtc cacaggataa ccgcgagcgc tactaccggg tctcatttcg
     6181 cgaggtcccc actcgtaacc agacaagacg cagcccaacc ggcggcgagg tcagcacgga
     6241 gccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc agtttaaatg
     6301 gtccttcgat caggtgaccg gaacggtaag taacaccggc aacacgtggt ttaagctact
     6361 gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg
     6421 agacgtggtt catcagcctg agttacgtca gccggggaat cattatctgg tctataacga
     6481 caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg actgatactt
     6541 atccatcggt cgagggaaga attccacctc agagactcaa aaaacgttta aatgatgtgg
     6601 aagcgatcgt caatgtcacc tgtaacgatg ggctttctca tatgcacacc atagtgttca
     6661 ggagacacag aaaggtatca ggctgcctgc taacgagtgg tttattcacg aaatagtacg
     6721 taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagta caccagccaa
     6781 ccgcggtacg ttcttacata cgatgtaccg ctgttctctt tacgatttat agctgtactg
     6841 gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg
     6901 cgtctctttt tactgggccg aagccgacga tgctggcggt accattcccg ccaggctcac
     6961 tgaacaaata tccccttttc accgatgcat gagcggtacc aatcagaaaa acccccgatg
     7021 tattgccctt gcaggaaccc caggcaaaaa tgcctgctgc acgatatata aaaatcgatc
     7081 gtccacgtgc agagaattcg ccatgtctgg tgagaacgga gaagtcaatg aggcttgcaa
     7141 tcgtgcaagg gctaaatacg ggctgacacc gttataaata tacaataatt aattgcactg
     7201 ccccgccagc gataatagcg gggcttcgtt ttcagggaca acaaaacccg aaacttcttg
     7261 tatttgccag caacaatgcc tctcttctgt agcgttcttc ggatctacat catcctaaag
     7321 tagcgcgata tactcactga ctatctgaat cagaatataa aaagcaatgt ttttatcatt
     7381 caatgcatta tctgtttgag cctaagggga tctcagggtc tggctcatga gtaattctgt
     7441 ttcgtaaatc tctgcgaata atttcaatgg accagaacca gactaaatgt ccaaaaattt
     7501 cagaaacatt ctcataccac gggagatcaa aaagaggtgg tgtcagtccc atgagaggga
     7561 atgaaatcat atgaacaaaa agttgggcta aagcacctgc cagtaagccc tgccagagtt
     7621 taatttttgg aaatacttca gcgaccacac aataaccgac agcaaacact atcgaaaaga
     7681 taatgtgcgt aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat
     7741 tgggatctgt cagccccagc cagtctcgaa gaaaaatata cggaggattg agaaaattac
     7801 gcgagcaatc aatttgccca gcagccctga ttaatgattc cgggccacac gctgcattaa
     7861 acatatccac cgggctacgt ggcggcaatg gaacttcagc cccccacttc acaaatgcgg
     7921 aaacaacgcc agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg
     7981 gagtttgttc aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttt
     8041 aggagtattc ataaaacaag gtaaatgtaa agaactgtat tgttttgtat aacaagatgg
     8101 ttgtctaatc gccaaagaat ataagcacca tcatttctcc ctatttttat attagaagtg
     8161 acatagattt gcagggtgat gcagagctga caatcacagg tttccttatc ggtttttgca
     8221 tcgtacaact caagcaataa accagctccc atcatcatca acatcccggc gaacatgaat
     8281 ttattagccg ggctcatagc tgcattcgcg ttttaagaat aatcctcctg ctgtcgccga
     8341 ctatgcttaa cgtttaaaaa agcatcagca ctctcgcaac gcactcttat tttccccttt
     8401 agaataccgg aggcctggta tgagcaacca aggcgaatac cccgaagata atcgggttgg
     8461 gaagcacgag ccgcacgatt ttagtttgac ccgtcgcgat ctgattaaag tgagcgccgc
     8521 aacggcggcg accgccgtgg tttatcctca ttctacgctt gcggcaagcg ttccggcagc
     8581 tacacccgcg ccagagataa tgcccctgac acttaaggtg aacggcaaaa cggagcagct
     8641 tgaggtggat acccgaacca cgctactgga cgctttgcgt gaaaatctgc atttgatcgg
     8701 taccaagaaa ggttgcgatc acggacagtg cggagcctgt accgtgctgg tcaatggtcg
     8761 caggcttaat gcctgcctga cgctggcagt catgcatcag ggggccgaga tcaccaccat
     8821 tgaaggcctg ggttcaccag ataatcttca ccccatgcag gcggccttta tcaagcatga
     8881 tggcttccag tgcggctact gcacctccgg gcaaatttgc tcatcagtag cggtgctaaa
     8941 agagattcag gacggcattc ccagtcacgt cacggtcgat ttggtttccc ctccagaaag
     9001 aactgccgat gagatccgtg aacgtatgag cggcaacatc tgtcgctgtg gtgcatacgc
     9061 taacatcctt gccgccattg aagacgctgc gggggagata aaatcatgaa ggcgtttacc
     9121 tatgaacgag tgaacacccc ggccgaggcg gcacttagcg ctcagcgcgt acccgacgca
     9181 aaatttatcg cgggcgggac caatctgctg gacctgatga agctggaaat tgaaacgccc
     9241 acccacctta tcgatgtgaa cggcctcggg ctcgataaga ttgaagtgac cgacgcgggt
     9301 gggctgcgca tcggcgcact ggtacggaac accgacctgg cggctcacga gcgcgtgcgt
     9361 cgtgattacg cggtactctc ccgcgccctg ctcgctggcg cgtctggtca gttacgcaat
     9421 caggcaacca ccgcaggtaa tctgctccag cgcacgcgct gcccctattt ttacgacacc
     9481 aatcagccct gcaataagcg cctgcccggg agcggctgcg cggcgcttga aggctttagc
     9541 cgtcagcacg cggtggtagg cgtaagcgaa gcctgcattg ccacccatcc gagcgatatg
     9601 gcggtcgcaa tgcggttgct ggatgcggtg gtggaaacca tcacgccgga gggaaagact
     9661 cgcagtatca cactggctga tttttatcac ccaccgggga aaacgccgca cattgaaacc
     9721 gccctgcttc ccggtgagct tatcgttgcg gtgacgttac ctccgccgct cggcggaaaa
     9781 catatttacc gtaaggtgcg cgatcgcgcc tcctacgcct ttgccctggt atcggtcgcg
     9841 gcgattattc agcctgacgg cagcgggcgc gtcgcgctgg gcggagtagc acataagccc
     9901 tggcgcattg aggctgcgga tgctcagcta tcccaggggg cacaggccgt gtatgacgcg
     9961 ctgttcgcca gcgcccatcc caccgctgaa aacaccttta aactcctgtt ggcgaagcga
    10021 acgcttgcct ccgtactggc tgaagcgagg gcacaggcat gaaatttgat aaacccgcag
    10081 gggaaaaccc gatcgatcag ctgaaggttg tcggtcgtcc ccatgaccgc atcgacggac
    10141 cgctgaaaac taccggcacg gcacgctacg cctacgaatg gcatgaagaa tcccccaacg
    10201 ccgcctatgg ctatatcgtc ggttccgcca ttgccaaagg acgcctcacc gcccttgata
    10261 cggacgccgc gcaaaaagcg ccgggcgtac tggctgtcat taccgccagt aacgccgggg
    10321 cactcagtaa aggcgacaaa aacaccgcca ggctgttagg cggccccact attgagcact
    10381 atcatcaggc cattgcgctg gtagtggccg agaccttcga acaggcgcga gcggcggcct
    10441 cgctggtgca ggcgcactat cgccgtaata aaggagctta ctccctggcg gacgaaaaac
    10501 aggccgtcag tcagccgccg gaagacacgc ccgacaaaaa cgtcggtgac tttgacgggg
    10561 ctttctcctc cgctgcggtg aagattgatg ctacctacac gaccccggat cagagccata
    10621 tggcgatgga gccgcatgcc tcgatggccg tctgggatgg aaataagctt actctctgga
    10681 cctcaaatca gatgattgac tggtgccgca ccgatctggc aaaaacgctg aaagtgcccg
    10741 tggagaatgt gcgtattatc tccccgtata tcggcggagg gtttggcggc aagctgttcc
    10801 tgagaagcga tgcgctgctg gcagccctcg ccgcccgagc ggtgaaacgt ccggttaaag
    10861 tgatgctccc ccgccccact attcccaata acaccacgca ccgccccgcc acccttc
//
LOCUS       DAZGCQ010000145        10904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000145 DAZGCQ010000000
VERSION     DAZGCQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.145"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1145
                     /gene="yghO"
                     /locus_tag="ACHK07_004486"
     CDS             3..1145
                     /gene="yghO"
                     /locus_tag="ACHK07_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO8104731.1"
                     /translation="MERDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1152..1916)
                     /gene="glcC"
                     /locus_tag="ACHK07_004487"
     CDS             complement(1152..1916)
                     /gene="glcC"
                     /locus_tag="ACHK07_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO8104732.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLSASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2167..3666
                     /gene="glcD"
                     /locus_tag="ACHK07_004488"
     CDS             2167..3666
                     /gene="glcD"
                     /locus_tag="ACHK07_004488"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO8104733.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHVGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSGEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3666..4718
                     /gene="glcE"
                     /locus_tag="ACHK07_004489"
     CDS             3666..4718
                     /gene="glcE"
                     /locus_tag="ACHK07_004489"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO8104734.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4729..5952
                     /gene="glcF"
                     /locus_tag="ACHK07_004490"
     CDS             4729..5952
                     /gene="glcF"
                     /locus_tag="ACHK07_004490"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO8104735.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKSDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5957..6361
                     /locus_tag="ACHK07_004491"
     CDS             5957..6361
                     /locus_tag="ACHK07_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO8104736.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6383..8554
                     /gene="glcB"
                     /locus_tag="ACHK07_004492"
     CDS             6383..8554
                     /gene="glcB"
                     /locus_tag="ACHK07_004492"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO8104737.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMASGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8909..10591
                     /gene="glcA"
                     /locus_tag="ACHK07_004493"
     CDS             8909..10591
                     /gene="glcA"
                     /locus_tag="ACHK07_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO8104738.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2552 a         2748 c         3046 g         2558 t
ORIGIN      
        1 ctatggaacg cgatctgctg atgattaaaa ttgaaaaagt aattaataaa aatgacctta
       61 aggcatttat cgcttttccg tcatcacttt atcccgacga tccaaactgg ataccccctt
      121 tattcattga acgcaacgag catttgtctg cgaaaaatcc ggggacggac catatcatct
      181 ggcaggcgtg ggtggcaaaa aaagcggggc agatagttgg gcgtattacc gcgcaaatag
      241 ataccttgca ccgcgagcgt tacggcaaag ataccggtca tttcggcatg attgacgcca
      301 ttgatgatcc gcaggttttt gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac
      361 aaggtgcaag taagatcagc ggtccattca gcctgaatat caatcaggaa agcggattac
      421 tgattgaagg tttcgataca ccaccctgtg cgatgatgcc acacggcaaa ccgtggtatg
      481 ccgcgcatat tgaacaactg ggttatcaca aaggcattga tttactggcg tggtggatgc
      541 agcgaaccga tctcactttc tctccggcgc taaaaaaact gatggatcag gtgcgcaaaa
      601 aggtgaccat tcgctgcatc aatcgtcagc ggtttgccga agagatgcag atcctgcgtg
      661 agattttcaa ctctggctgg cagcacaact ggggatttgt gccgtttacc gaacatgaat
      721 tcgcgaccat gggcgaccaa cttaaatatc tggtgccgga cgatatgatc tacatcgctg
      781 agattgattc tgcaccctgc gcgtttattg tcggcttgcc gaacatcaac gaggcgattg
      841 ccgatctgaa cggatcgctc tttcccttcg gctgggcaaa attgctgtgg cgtttgaaag
      901 tcagcggtgt gcgaactgcg cgagtaccgc tgatgggcgt acgcgacgag tatcagttca
      961 gccgcatcgg cccggtgatt gcgctgttat tgattgaagc cttacgcgat ccgtttgccc
     1021 gccggaagat cgatgcgctg gagatgtcat ggatcctcga aaccaataca ggcatgaata
     1081 acatgctgga acgcattggg gcggagccgt acaagcgtta ccgattgtat gaaaagcaga
     1141 tttaatacca gctaactcag gttcatctcc agcggcaccg ccgagcgaat caaatggtgg
     1201 ccttccagct cgatatcgtg gagattcttt ttcacggtcc gcacatgatc gcgtgctgcg
     1261 cgctgggcga cgtgcggcag ccgctgcaac accgcgttgt agatccgcgc atgctggcga
     1321 tcgatctgct ttttttgctg tggtcgatgg tagagattat ttacgctggc aaacactgaa
     1381 ttaaacatca gatcggtcaa tgattgcagc gtaaacacca gcacctgatt gtgagaagcc
     1441 tgacaaatgg cgagatggaa agcgtgatcc aactgcgcat gttcgatcag cgaaatctct
     1501 ttgttgttct cactggcgga gagcattttt tcataacagc gagttatcac aacaaaatca
     1561 gcctgcgttc ccagcgttgc cgccagcctt gccgattcgc cctccagtaa tgcgcgaaca
     1621 tcgagcagat cgtacagcgt tcgcggttgc gtactgaaca gatggatcag cggactggtg
     1681 tcctgcaccc gattaagccg tgcgacacga gaatcgcgac cctgcgccgt ttcaataatc
     1741 ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg agcgtgagaa gccgagcttt
     1801 tcacacagtc gacgttccga gggaagcggc tgaccgacct tcagtacgcc gtcgataatt
     1861 aaccgttcga tactctctgc aaccacttcg caaatagggc gacgttcatc tttcattccc
     1921 ggacctcgtg cacaggtagg accaattttt ctgttcttaa ctcgcaaaac acgcacatca
     1981 cgtaagtgat tatgttacat caatttaaca ttaagttaac caaggcaagg tcacagagct
     2041 ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg aaaacgaaaa gcccgcttaa
     2101 taactgttca cagaagcagc gcgcaaaaat cagctgccac acaacataac aaagcgaagc
     2161 ctactcatga gcatcttgta cgaagagcgt cttgatggcg ctttacccga tgtcgaccgc
     2221 acatcggtac tgatggcact gcgtgagcat gtccctggac tggagatcct gcataccgat
     2281 gaggagatca ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg tccattactg
     2341 gttgttctgc ctaagcagat ggaacaggtg acagcgattc tggctgtctg ccatcgcctg
     2401 cgtgtaccgg tggtgactcg tggtgcaggc accgggcttt ctggtggcgc gctgccgctg
     2461 gaaaaaggtg tgttgttggt gatggcgcgc tttaaagaga tcctcgacat taaccccgtt
     2521 ggtcgccgcg cgcgcgtgca gccaggcgtg cgtaacctgg cgatctccca ggccgttgca
     2581 ccgcataatc tctactacgc accggaccct tcctcacaaa tcgcctgttc cattggcggc
     2641 aacgtggcag aaaatgccgg cggcgtccac tgcctgaaat atggtctgac cgtacataac
     2701 ctgctgaaaa ttgaagtgca aacgctggat ggcgaggcac tgacgctggg atcggatgcg
     2761 ctggattcgc ctggttttga cctgctggcg ttgttcaccg gatcggaagg tatgctcggc
     2821 gtgaccaccg aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg ggttctgtta
     2881 gcaagctttg actcggtaga aaaagccgga cttgcagttg gtgacatcat cgccaatggc
     2941 attatccccg gcgggctgga gatgatggat aacctgtcga tccgcgcggc ggaagatttt
     3001 attcatgtcg gttatcccgt cgacgccgaa gcgattttgt tatgcgagct ggacggcgtg
     3061 gagtctgacg tacaggaaga ctgcgagcgg gtcaacgaca tcttgttgaa agcgggcgcg
     3121 actgacgtcc gtctggcaca ggacgaagca gagcgcgtac gtttctgggc cggtcgcaaa
     3181 aatgcgttcc cggcggtagg acgtatctcc ccggattact actgcatgga tggcaccatc
     3241 ccgcgtcgcg ccctgcctgg cgtactggaa ggcattgccc gtttatcgca gcaatatgat
     3301 ttacgtgttg ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt aatccttttc
     3361 gatgccaacg aacccggtga atttgcccgc gcggaagagc tgggcgggaa gatcctcgaa
     3421 ctctgcgttg aagttggcgg cagcatcagt ggggaacatg gcatcgggcg agaaaaaatc
     3481 aatcaaatgt gcgcccagtt caacagcggt gaaatcacga ccttccatgc ggtcaaggcg
     3541 gcgtttgacc ccgatggttt gctgaaccct gggaaaaaca ttcccacgct acaccgctgt
     3601 gctgaatttg gtgccatgca tgtgcatcac ggtcatttac ctttccctga actggagcgt
     3661 ttctgatgct acgcgagtgt gattacagcc aggcgctgct ggagcaggtg aatcaggcaa
     3721 ttagcgataa aacgccgctg gtgattcagg gcagcaatag caaagccttt ttaggtcgcc
     3781 ctgtcaccgg gcaaacgctg gatgtgcgtt gtcatcgcgg cattgttaat tacgacccga
     3841 ccgagctggt gataaccgct cgtgccggaa cgccgctggt ggcgattgaa gcggcgctgg
     3901 agagcgccgg gcaaatgctt ccctgcgagc cgccgcatta tggtgaagaa gccacctggg
     3961 gcgggatggt cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt tcggtccgcg
     4021 attttgtcct cggcacgcgc atcattaccg gcgctggaaa acatctgcgt tttggtggcg
     4081 aagtgatgaa aaacgttgcc ggatacgatc tctcacggtt aatggccgga agctacggtt
     4141 gtcttggcgt gctcactgaa atctcaatga aagtgttacc gcgaccgcgc gcctccctga
     4201 gcctgcgtcg ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag tggcaactcc
     4261 agccattacc cattagtggc ttatgttact tcgacaatgc gttgtggatc cgccttgagg
     4321 gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag gttgcgggtc
     4381 agttctggca gcaattgcgt gaacaacaac tgccgttctt ctcgttacca ggtaccttat
     4441 ggcgcatttc attacccagc gatgcgccga tgatggattt acccggcgag caactgatcg
     4501 actggggcgg ggcgttacgc tggctgaaat cgacagccga ggacaatcaa atccatcgca
     4561 tcgcccgcaa cgctggcggt catgcgaccc gctttagtgc cggagatggt ggctttgccc
     4621 cgctatcggc tcctttattc cgctatcacc agcagcttaa acagcagctc gacccttgcg
     4681 gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg agcaggctat gcaaacccaa
     4741 ttaactgaag agatgcggca gaacgcgcgc gcgctggaag ccgacagcat cctgcgcgcc
     4801 tgtgttcact gcggattttg taccgcaacc tgcccaacct atcagcttct gggcgatgaa
     4861 ctggacgggc cgcgcgggcg catctatctg attaaacagg tgctggaagg caacgaagtc
     4921 acgcttaaaa cacaggagca tctcgatcgc tgcctcactt gccgcaattg tgaaaccacc
     4981 tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat tgtcgagcag
     5041 aaagtgaaac gcccactgcc ggagcgaata ctgcgcgaag gattgcgcca ggtagtgccg
     5101 cgtccggcgg tcttccgtgc gctaacgcag gtagggctgg tgctgcgacc gtttttaccg
     5161 gaacaggtca gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg tccgccgctg
     5221 cgccataagc gtcgggtttt aatgctggaa ggctgcgccc agcctacgct ttcgcccaac
     5281 accaacgcgg caactgcgcg agtgctggat cgtctgggga tcagcgtcat gccagctaac
     5341 gaagcaggct gttgtggcgc ggtggactat catcttaatg cgcaggagaa agggctggcg
     5401 cgggcgcgca ataatattga tgcctggtgg cccgcgattg aagcaggtgc cgaggcaatt
     5461 ttgcaaaccg ccagcggctg cggtgcgttt gtcaaagagt acgggcagat gctgaaaagc
     5521 gatgcgttat atgccgataa agcgcgtcag gtcagtgaac tggcggtcga tttagtcgag
     5581 cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa actggccttc
     5641 cactgcccgt gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga aaaagtgttg
     5701 cttcgtcttg gatttacctt aacggacgtt cccgacagcc atctgtgctg cggttcagcg
     5761 ggaacatatg cgttaacgca tcccgatctg gcacgccagc tgcgggataa caaaatgaat
     5821 gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca acattggttg ccagacgcat
     5881 ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg aaattgtaga acaagccctt
     5941 gaaaaggaat aacaaaatga aaactaaagt cattcttagc cagcaaatgg cgagtgcaat
     6001 tattgccgca ggtcaggaag aggcgcagaa aaacaactgg tctgtttcca ttgctgttgc
     6061 cgatgacggc ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc cgattgcggc
     6121 ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa ctaagggcta
     6181 tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact gcgccgttat taacgtcgct
     6241 ggaaggcggc gtaccggttg ttgttgatgg gcaaattatt ggtgccgtgg gcgtttctgg
     6301 tttaaccgga gcacaggatg cgcaggtcgc gaaagcggca gcagcggtgt tggcgaaata
     6361 agcgaaaacg aggagagaaa caatgagtca aaccataacc cagggccgtt tacgcattga
     6421 cgccaatttt aaacgttttg tggatgaaga agttttaccg ggaacagggc tggacgctgc
     6481 ggcgttctgg cgcaattttg atgagatcgt tcacgatctg gcaccagaaa atcgtcagtt
     6541 gctggcagaa cgcgatcgca ttcaggcggc gcttgatgag tggcatcgca gcaatccggg
     6601 gccggtaaaa gataaagcgg cctataaatc tttcctgcgt gaactgggct acctggtgcc
     6661 gcaaccggag cgcgtgacgg tggaaaccac gggcattgac agcgaaatca ccagtcaggc
     6721 ggggccacag ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg cggcgaacgc
     6781 tcgctggggc tcactgtacg atgcgttata cggcagcgac atcatcccgc aggaaggggc
     6841 gatggccagc ggctacgatc cgcaacgcgg tgagcaggtt atcgcctggg ttcggcgttt
     6901 cctcgatgaa tctctaccgc tggaaaacgg cagctatcag gatgtggtgg cgtttaaggt
     6961 ggtcgataaa caattacgca tccagttgaa aaatggtaaa gaaaccacgt tacgtacccc
     7021 ggcgcagttt gtcggttacc gtggcgatgc cgctgcgccg acctgcattt tgctgaaaaa
     7081 taacggcctg catattgaac tgcaaattga tgccaacggg cggattggca aagacgatcc
     7141 ggcgcacatc aacgatgtta tcgtcgaagc ggccatcagt accattctcg actgcgaaga
     7201 ttcggtcgcg gcggttgatg cggaagataa aatcctgctg taccgcaacc tgctgggcct
     7261 gatgcagggg actctgcaag agaaaatgga gaaaaacggt cgacaaatcg tgcgtaaact
     7321 gaatgacgat cgtcattaca ccgccgccga tggctctgaa atttctctgc acggacgctc
     7381 gctgctgttt atccgcaacg tgggtcattt gatgaccatt cctgtgattt gggacagcga
     7441 aggcaatgaa atcccggaag gcattcttga tggcgtcatg actggcgcga ttgccctcta
     7501 tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga aaccgaaaat
     7561 gcacggtccg caggaagtgg cgttcgccaa caaactgttt acccgcattg agacaatgct
     7621 cggtatggca ccgaataccc tgaaaatggg cattatggat gaagaacgtc ggacctcgct
     7681 gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca ataccggttt
     7741 cctcgaccgt accggcgatg aaatgcattc ggtgatggaa gctggcccga tgctgcgtaa
     7801 aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa cgtaataacg tgctttccgg
     7861 tctgttctgt gggctgcgcg gtaaagcgca aattggtaaa ggcatgtggg caatgccgga
     7921 cctgatggca gacatgtaca gccagaaggg cgaccaactg cgtgccgggg caaacacagc
     7981 ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg cactaccacc aaaccaacgt
     8041 acagagcgtg caagccaaca ttgcccagac cgagttcaat gctgaatttg aaccgctgct
     8101 ggacgatctg ctgactattc cggttgctga aaacgctaac tggtcggcgc aagagatcca
     8161 acaagagctg gataacaacg tgcaggggat tctggggtac gtggtgcgct gggtggagca
     8221 ggggattggt tgttcaaaag tgccggatat tcacaatgtg gcgctgatgg aagaccgcgc
     8281 aacgctgcgt atctccagcc agcatatcgc caactggtta cgtcacggta ttctgaccaa
     8341 agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc aaaacgctgg
     8401 cgatccggct tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt ttaaagctgc
     8461 cagcgatttg atcttcctcg gcgtgaaaca gccaaacggt tataccgaac cgttattaca
     8521 cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc ccggcaagtt
     8581 ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc
     8641 gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg cctaaaacaa
     8701 caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa cccttacccg
     8761 atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc tgtaaagtat
     8821 aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag tatatcaagc
     8881 atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt atatgccgat
     8941 gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct tcttcgttgc
     9001 actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta tattatctat
     9061 cctgattgca atattcgcct ttaaaatgcc gattgatatg gcatttgctg ctgcgggcta
     9121 tggctttatt tatggattat ggccaattgc atggattatt gtcgcggcgg tattcctcta
     9181 taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta tctccatcac
     9241 cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt tgctggaagg
     9301 agcggctggc tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg gcctgggctt
     9361 caaaccgtta tacgcggcgg ggctgtgtct gattgccaat actgcgccgg tggcgtttgg
     9421 tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat
     9481 tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt tctggctggt
     9541 agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg
     9601 gggaagcttc gctgtcactc agttctttac ctctaattat attggtccgg aactgccgga
     9661 tattacttcg gcgctggtga gtatcgtctc actcgcttta ttccttaaag tctggcggcc
     9721 gaaaaatacc gaaacggcaa tcagcatggg acaatccgca ggtgcgatgg tggtaaataa
     9781 gccatcttct ggcggtcccg tgccttcaga atatagtctg gggcaaatca ttcgagcgtg
     9841 gtcaccgttt ttaatcttaa cggtgctggt caccatctgg accatgaagc cgtttaaagc
     9901 gttatttgct ccgggcggcg cgttttattc actggtgatt aatttccaga tccctcattt
     9961 gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt
    10021 gtttaaattc gaccccctct cggctggcgg caccgctatt tttattgcgg cgattatctc
    10081 tatcttcatc ctcggtgtgg ggatcaagaa aggtattggc gtctttgccg aaacgctaat
    10141 tagtctgaag tggccgatac tgtcgattgg catggtgctg gcgttcgcct tcgtcaccaa
    10201 ctattctggc atgtccacca cgctggcgct ggtactggca ggtacaggcg tgatgttccc
    10261 gttcttctca ccgtttctcg gctggctggg cgtattcctt accggctcgg acacctcctc
    10321 taacgccctg tttggttcac tgcaatcgac cacggcgcag caaatcaacg tctctgacac
    10381 tctgctggtg gcagcaaata ccagcggcgg cgtaactggc aagatgatct ccccgcaatc
    10441 tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga gaatctgaac tgttccgcta
    10501 caccgtgaag cacagtctga tttttgccag cgttatcggc attatcaccc tgctgcaggc
    10561 gtatgtgttt accgggatgt tagtctcgta aatcaataaa gccggatgat cgtcaatatc
    10621 atccggcttc atttacatta ttctgtctta ttaattgaaa atcatttctc gctttattat
    10681 ccatgcagca ataatgtttt gcgttcagat aacctgctaa ataattaaga gcgtgagaac
    10741 atctattttt ttatcacgca aaacaagtct gtattaatta tgttcgcttg atgagcaaac
    10801 accgttctat ctgttgcttt tgcaatctta atcgttaaaa ctaccaacga atttatatag
    10861 ttacgttgtg aaatgttgtt aattttttgt ttattgaaat ttcg
//
LOCUS       DAZGCQ010000146        10740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000146 DAZGCQ010000000
VERSION     DAZGCQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.146"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..401)
                     /gene="tssJ"
                     /locus_tag="ACHK07_004494"
     CDS             complement(<1..401)
                     /gene="tssJ"
                     /locus_tag="ACHK07_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8104739.1"
                     /translation="MAIIAGKTGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAA"
     gene            complement(382..1482)
                     /gene="tssG"
                     /locus_tag="ACHK07_004495"
     CDS             complement(382..1482)
                     /gene="tssG"
                     /locus_tag="ACHK07_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8104740.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYPGSRLDVRLQLCVKRSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTISLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(1437..3200)
                     /gene="tssF"
                     /locus_tag="ACHK07_004496"
     CDS             complement(1437..3200)
                     /gene="tssF"
                     /locus_tag="ACHK07_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8104741.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KIAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVENVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQPWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVRNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(3208..3900)
                     /locus_tag="ACHK07_004497"
     CDS             complement(3208..3900)
                     /locus_tag="ACHK07_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8104742.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGIFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFEAWLK
                     SERESPEQLALF"
     gene            complement(3902..5500)
                     /gene="tssA"
                     /locus_tag="ACHK07_004498"
     CDS             complement(3902..5500)
                     /gene="tssA"
                     /locus_tag="ACHK07_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8104743.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADEVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLAELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            complement(5500..8889)
                     /locus_tag="ACHK07_004499"
     CDS             complement(5500..8889)
                     /locus_tag="ACHK07_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8104744.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPVDGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTDDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPAIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVTVPESRLHGPLATTFGPVLALMDNQNNSADLLNLQTYLTRITQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLAAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNTRWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLRREGNRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPEMVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(8882..10030)
                     /locus_tag="ACHK07_004500"
     CDS             complement(8882..10030)
                     /locus_tag="ACHK07_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO8104745.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGTLLFIIRASGY
                     VKVLNDFSIWWLALTPSGCWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(10034..10300)
                     /locus_tag="ACHK07_004501"
     CDS             complement(10034..10300)
                     /locus_tag="ACHK07_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8104746.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(10332..>10740)
                     /gene="tli1"
                     /locus_tag="ACHK07_004502"
     CDS             complement(10332..>10740)
                     /gene="tli1"
                     /locus_tag="ACHK07_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO8104747.1"
                     /translation="EHLGATESYYNKGKALPLMLRFCWDSIIDKKSYETLIFFKKGTW
                     EQMVTPYTDNRWNETYYRSTMIIGLAPEGKVRVWLGDRGNPVVPQLDAKITTVSGDKM
                     NMCKGVTKSDFTYGYDEDIKEFIKDKTYPYGNW"
BASE COUNT         2743 a         3056 c         2483 g         2458 t
ORIGIN      
        1 gctgccacac cggtaaattt cgccgcatca tccagaggca tatccacggc cactgaacca
       61 cctggctgca gccatacatc tttctgtgca atgatatcgc ctgccagaat ttcactgtcc
      121 cccgtaaaaa gcgcctgata atccgcgctg tcaaaattcc ggttgtcttt aagctgataa
      181 atccgcacca caaccgacag gggaataccg gcagcactgg tgttaatggc ctccctggca
      241 cgaatatcaa ggtgaagtgt ttttatctgt cggtaaaaca gggatttcgt ggcagatacc
      301 gtaccgtctg ccactctttg cgtcaggcca caccctgata acgacaggga aaacaacgca
      361 ataatcagac cgtaaccagt cttaccagcg ataatcgcca ttttcctgag cctccttacg
      421 ttgataatgt tcctgaaccc gctgataacg ccccagactg atggtcatta cgcgtgccgc
      481 tgaagtcgtt atctttgcct gtgttctcat tacagccgtg cgccccagtt gcggactacc
      541 ggctgccggt cggcaactga gccggacatc cggcaacagt gaacgcttaa cacaaagttg
      601 cagacgtaca tccagacggc ttcccggata aacgtgcaac aacgccagca aatcagaata
      661 cagctgcccg cccggtaacc agccctgtac ctcgctgccc gtctgtgttg aaagctgtaa
      721 taacacctgt ccgttcacat ccgtagcatg atcgcccatc accgggcgac tcttcagact
      781 gaccggatga tgaacacgca tggccagcgg tgttttcagc gggatccgtc gtcggtcatg
      841 atgccagaca cgggcctgtg ttccgggagc cagcagcgtt accagtgatg tcagcccttc
      901 agctgttctt ccgggtaaca acatcagggg caataacgcc agaaaacgcg ataccggcgt
      961 ggcaatattc tgtgcacatc cggggatccc cagtctggcc agaccgagca gatactggga
     1021 tgttttatcc tgcccccccg cctcaaaggt ggccgggtac gaatatttgc gccagatacg
     1081 gtaatactgg gtgatcagac gatgactgaa tatatcgaga aaatccgctg cggcttcatg
     1141 gccttcccgc tgctgtgcaa tatcactgat atagtgagcc ggcaacggag acgtgacccc
     1201 gtaaagtccc ataaacgtca catgtacagt gggtggcaga tgggattctt caggaattac
     1261 agcatctttt atttcactcg caggaaaccc cattcccgtg tatggacaaa agcgaaccgc
     1321 ttcctgacgg gcctgccagc cactgccaat gggaggctga tctggctggc tttgttccag
     1381 taactgacag aagcgataaa aattaatata cggaagctgt ttccggtaac gggtaattaa
     1441 ccgggcaggc gcggattgtg attttctttc caccggatgc attttccttc aggctgaaca
     1501 atcagtgtga gctgattgaa ctgattcata tcggcgtaca gggccaggaa acggttgagc
     1561 atttcgccaa acaggtgaat atctccctct ccggcaaacc cgttgccatc aagagttacc
     1621 tccacatcaa gcccccggag cagaaaaccc ttttcaaaac gctgaatacg atgatgctgt
     1681 acagccagaa tggcatcaag ccgacggtga ttaagttcat cgtcccgcca gttataaagc
     1741 gccagcgtgc cacgcagcac ttccgcactg ctcagcatgt taagaaatcc tgtccccaga
     1801 tgactcatca cccgccagtg aaaacgatct tctgtcggcg gataaaccgg tagcgtgggt
     1861 ttacaaagat tacgtacact gaccggggtc tgcagtacct gttcacaccg atcaagcagc
     1921 gtactctgca acgcacgacg gggaagctga ccattggtac ctgtaatacg taatgaaagc
     1981 gtttcccgct ccggcatccg atccacctcc caccgctgac cgccaaggat aagccatgtg
     2041 tcatacatcc ccgtgacacc ccgcttcacc cgggtatgat aataacgttc gggtgcatca
     2101 tggcgcagca ttcccccacg gtgacggaag ctggtaaaag gcacgtactc tgcactgccg
     2161 gttcgcccgg agcccgtcac cgcatccact gaatatatct ccgtataccc atcctgcaga
     2221 cgcttcgggc gcagcaggta ttcgctctcc agcccattga tgatgagtgg atcggcttca
     2281 agcgtaaaaa ggttaatcac cggcacacaa tgcagacaca gggcatcgtc tgtcacaggt
     2341 aaatctgccg gccagggctg actcagcacc acctcaagat caaatccgga aatacctgcc
     2401 ggcagagaaa cattctccag tccgttcaga tgaacaaaca taaacttttc ccgaaaggtg
     2461 aaatattcca gcaataactg atacccactg aaagcactgt cacctttcgg ccacagccga
     2521 tcttcttctg caaaaccgcc gggtgaaaac catccgtcaa tgcggatccg ctcatcattc
     2581 cccggcagcc tcagatacag cgcagccaga cgctttgtca tcatcagatg aagggtgctg
     2641 ctgaccgggg cttctgctgc cagatacaga ctcagacgat gcaaatcgac ctgagaccag
     2701 tcagccagtt cactgcaggc aaaacctatt ttcagcactg agcgcccgtc cggctccgtc
     2761 gtcataacga cattctccac tgccagtgga ttcagtggta tggcccgggt ggtacggtaa
     2821 cgacagactg tatttccggg gccaacaggg cgggaagtca cttccagtcc ggctggtacg
     2881 gtttcagcta ttttcatgac ggataaccgg ggggtcagtg ccaccaccga cagggacggg
     2941 atagtacgca ggtaatgagg ccagagcata ctgaccagac tttcagtaag ctccggcaaa
     3001 tcatcgtcga ttttctggcg cagtcgtccc atggaaaagg caaaaccttc aaacaggcgt
     3061 tccacgcagg gatcaggcgt acccgcttta tcgagatcca gcatcgctgc ccgatcggga
     3121 tgagcctgtg caaacgcctt tccggcttca cgcagataac gcatttcagc gtcaaaataa
     3181 cgcagggtta aatcgtccat tatcttctca gaataaagct aactgttccg gagactcgcg
     3241 ctctgacttc agccatgcct caaacaatcg cccgaactga acggcaagcc ggatttcatt
     3301 aatcagatta catgtgaata taactgtcag aaatatcatg ataaaataaa cgagggaaaa
     3361 taacataaat aaaactgtat taccccccga cagaaatggc atggttgtaa atatccctcc
     3421 cgtaaactga acacataaag aaaacaccgc aaacactgct atcatccggt aacctttatg
     3481 cacacgacga gtattaacag taaatttgcc atctgaaaat ataccgacaa aagaatattt
     3541 aagaaaacaa tgcagtctgt tatccactga actgacattt ctgtcggata taaaacaaat
     3601 cagcttatta caacaccata tcggaagata tatccccatc tgttcataac agtattttat
     3661 tcttaccagt gtatttttcg ttgttggctc ttttgtcagt ttgtcaatac gggcaagaat
     3721 ggactccttt tcagatacag gaattttctc aaatctgtat ttcctgatat tcggcgcata
     3781 gccataatgc tgcacaaacc atttgacaag aggaataata atgcccgaca gaagcggtaa
     3841 caccagattt ttaactatat cctgaaatgt tttgtcctga agataatgca tgacagacat
     3901 ctcatgagct ccccggattg cacaacacag cagctctggc agcatcaatg gcgatgagcc
     3961 cggcaagaag atgttccata tcaggctgca gacgcaccct gtcactttca gtttttgcgg
     4021 acttcatacg taacagtttc aggcggcggg cttttacctc aaataccagt tcaggttccc
     4081 attgtgacag ggtcagccgg gttgcacgtt catccagctc cgcaagcaga tgcagggcaa
     4141 ggtcattttt tccagtctgt tcggcaacac gggccatcaa caggcgcagt agccagttgc
     4201 tgcggtcaga ctgaataccg ggacgtgcct gtaaccagct taaagcggct tctgttcctt
     4261 cattatccgc aatctgcagg gcttccggct caagagataa aatgtcgtta tctccggggg
     4321 ctgccgttat cgttacagac ggttcttccg cccagcgttc accacactcc atcacctgct
     4381 gctgtatcca gcttagcgtc acttcatcgg caaacggcat gccgtcgtta aaagccagtg
     4441 tctcaagccc cggcaggcgc agaagcaatc ctttcaggtc atactgaata atggcagcat
     4501 aattttcttt tccggtctgc aacagcgcct gatgaatata ccactgcagg tccagccaca
     4561 gatgactggc tccccgggca aacatatcgt cacactgttc aagcagtgac aaccagttct
     4621 gctgcagata caaccgtttc agtgacgcac gccgatccgg ccccggtggg gcaatacggg
     4681 tccgtccatc agcagataac ggcggtaact gatgcagcgt gtcatgccgg acgcttttca
     4741 tcagtcgatg agccgccagc cagccctcag gctggtcacg cagatatttt gccagaacac
     4801 gggcctgtga cagcagttcc tgtccggagg tgatggcaga taatgccatg acgctgccgg
     4861 atgttaccgt cggtgccggc gcggcagcct cctgaggcac cactgcatca acaccaccgg
     4921 ccttcatcag gcgattttca agtgcctgat ataacccgtt cagcaccggg gcggaagcct
     4981 catccgtaaa ggtctgttct gccagccaca gcgccccggc aatgcgcagg gtatccgcct
     5041 tcaccacctc cggatacaga gacagactgt ccagtatgcg ggcgctgcat aaccactcca
     5101 gcgccgcctt cctggcccgt gaccgttgtg gatgcaggtg ctctccaaaa cgctgcaaca
     5161 aaccggtcag cagctccaga ccgtcggcca gacctttttc accatcctgc cgcagacggg
     5221 cccagatata ccaggtcgcc acccggatat ctttggttct ggtggtcagt aatttttctg
     5281 caagctggct gacaattccg gcatcggctc cggagagttt attgacctct tcacgcatct
     5341 gctgaaaatc atcatcgtac cccgcatcat cacctgcggg attatctggc gtcacgggag
     5401 caagccattt atcccacagc gcaatacgca cctgagccgg ttcagccaca tcccggggtg
     5461 tcgtctggca tgcactgagg agcgaatgaa tggaagccat cagtcagtct cctccacgga
     5521 atccccggca tcttcgtcat tgcctgtaaa cgctgacgta ccggagagtt cgaaaaccat
     5581 ttccggcaga acaaaattgc ggagtttcag caatacaagc ggcccttccc ccgcttcagt
     5641 tcgcagtgtg taattcagca tacgaccgtc ctgcgcctgc cagctcaggc tccagccact
     5701 ggcaacgccc ggtgcggctt tccgtcgggc catctcaagc agacgtatca gcccccagct
     5761 tcccggcagg tctgcatact gacgggtacc ggcctgagtg cttacccagc ttaaactggc
     5821 gccaggtgct tccgtatcag ccggccaggt aaatcgcttc cataccggca tctgattaac
     5881 ataaatcagt ttctgattat cggttatcag cgtcgtctgc atcacaccgg cagcagttcc
     5941 cgggcgcagc tcaaaatgca gccccgcgtt acctgtggtg aacgccacat ctgctatttc
     6001 actcagggta ttgatggctt tcagaaatgc agggttgaat gtcagccccc gggtattaat
     6061 ggtatccggt acccagcgat tgccttccct gcgcaacaca ccactcaggt tattctgcag
     6121 aaaacgggca atacgtcccg tgtccgtatt caggtatttt gccagcaggg gaagtgaagc
     6181 atcgctgctg acatttttaa acggataacg cccggaaaat gcattattcc agccatccac
     6241 caccgcagta cgccaccggg tattcagact ttctgccgcg ggtgtaagaa cctgctgcca
     6301 cgcctgctcc atcgggcgaa caaagaccgt ctgtccgaag ccataccact cctgtccgag
     6361 tccggcagcg gccaggctgc cgtaatcccg ggtatcagtt aaatcaaccg atttcccctg
     6421 caatactgtc tgtgccagca tctgcatcat ggcctgcggg tcagaagagc cggcaatctg
     6481 ttgcagacgc agacgaacct gtgtaatccg ggtcagatac gtctgcagat tcagcagatc
     6541 cgcgctgttg ttctgattat ccatcagtgc aagcaccggc ccgaaagtcg tcgccagagg
     6601 accgtgaagc cggctttccg gtactgtcac tggctgtttt tcctgtgaaa gcaggtttct
     6661 ggcagattta accagtgaat cagtaaccgc ttcccggggc tgaccagtac gcccctgaac
     6721 cgccagcgta ttcatcagtg caaccagggg ggactggcgc acatcagcca tcagcgtcag
     6781 ttgctctgtc acatcagaca gcgtctgagc tttacgcaag tgcagactgt tcagaaaatt
     6841 cagccaggca ttaccaaaat cagcaaaata gcgcgttgtc aggcgctgtc tgagtgcttc
     6901 cggcgatacc ggtgacggtg ccttctgcct gccatccgtc agtacccagt ccatttcttc
     6961 acgacgctca ttaatcacgg tgtctatcgc cggcaatacg gcctcttccc aggcctggcg
     7021 ggtgaacata ccgggaacca cttcatccgt aaaaaagagg cgggagacat cagtatcatc
     7081 ggtcatatcc gtcagacgca tatccgcaaa ctgatgtgcc acccgtgcca gcattttctg
     7141 atacagcatg gcatcgctgt tctgtgtccc cagatggcga agcagcagag ttctgctctg
     7201 gctgaccagc tcttcatcag ccgtcagctt ccactgagga tgagtaataa tcccggaggc
     7261 ataatacgcc agcaacgtcg ggccattagc ctgccagaaa acggcactca caccgtcacg
     7321 ctgtggccat tcccgcatga gcgtacgact gaaccaggcg ggctccatat gttgcggttg
     7381 cgccagcatc agatacagtc tgagctggtc atatgcggct tttgccattt tcccccgctg
     7441 cgggctgtcc ggaggaagac gaataaatgt cctgagctgc tgctcaagat gagcagcagc
     7501 ggcatcacgc agtaaaagat gtgcattttg tgcataaagg ggcattacga ccgcgagcag
     7561 gtctgcattc tgattcatac cggcccgcag gtaccatggc gccccgtgct cctcccggta
     7621 ctgaaggcgg gccagggatt tctgcagatt cagcaacgcc tgcagacgtt ctgcaggaga
     7681 cagttgctgg ttctgagccg gacgaatctg ttcctgcact tcagcaacca gactccggtt
     7741 tgccagaaag gaaaccacca ttcctgttcc ctgagccatc atcagtatgg ccgccataac
     7801 ggccagactt ctgcgccagt tatgacctgt gcgggaaggc tgtagccgga caggtaattg
     7861 ctgcactgtc tccggcaacg cctcccagcg gttatccatt cgccagcgat gtctgacact
     7921 tcgttcaccg ccgacagttg ccggactgaa aacaataccc gccagcaaca actgacggta
     7981 tggacggagt aatacggata atggctccac cacagcatca atatttcgtc tgaatcgctc
     8041 tgccagcgac aacaaaaaat aataccgagg ggcacagcat atctgctgta tcccctgagc
     8101 aacaagcccg gggagcatgg cctgtagttg tgctttcagt ttgtcagaac tacattctgc
     8161 cggcagaagg catcccacgg gctgagtaat acgccctcgc tcatccggac tctcctgcag
     8221 tgaccagaca tacagcggca gacgccagcc aaggcgctca caacatgaac tgaccgcacg
     8281 ggaaaccctg tccagggcat cttctgtcag tggtgcggat aatgtttcac tcagtccgga
     8341 agttacccag acaatgccat caacaggccg gtaacgcaga cggcgtaacg cagccagcca
     8401 gtctgcgttt tccggctgcg aggggtctcc tccccagaga agcaatgtcc cctgctcttc
     8461 ctgccagaac tgttctgtca ggcccggggt cagcaactca acctcagagg cagtacctgt
     8521 tatcaacaga atgcgtattt tacgtcccca gaatctgcca tactggcgcc gcagagtatg
     8581 acgaatatcc tgaacagtaa catagcgcgg tggcgtctga gccagtcgct cttcatcgtc
     8641 aggtaacctt tgctccttcc gggcccgacg tcgatatgtc agttcctgaa catatttttt
     8701 catcacaggc aggtaacaga caaagagcaa ccccagactc agcaatatca gcagtagtat
     8761 ttgatcccgg cgtgtttctg cccccagaaa tgctccatat cgggttatca gccaccagag
     8821 cgcagccaca ccaataaata caatccccgg tcgtccaaag cgaccagaca gacaggccag
     8881 tttattcatt ctggtttcct gatgtaacag tactgataaa atgttcttct ccggatgaga
     8941 ataaagtcag gagagaatca ttaccaagac agaccagatc ggctgccaga gcagtaagca
     9001 gccacggagc agcaggtccc gggataccgc accatttttc cagtaacata cgtccggacg
     9061 gttcccatcc ggcaccatac tggtttccaa tagtcatcag cggtgcagca atcttttccc
     9121 agtgataaca gtcccccagt acacgagcgg tacgacaggc agctgtctgg gtttcaagga
     9181 aaagtgtgaa ttcatcatta aacttgttga tatccagcga catcgggcgt agcagacggg
     9241 ctggatgcgg caggttatat ttttgcgcaa catcatcgct ggtcaacaaa agtgcagcca
     9301 gtccgtcaga gtatgcattt cccccattaa gctgtattac cagtatcaaa tccacagtca
     9361 gcactggttg tttcagacgc tcatcaaccc accccatgga aaagtcggga gtaacctcga
     9421 tattgtcagg aacgacgcgt tggggaaata acgcttccca tgctgaaaca aaagcagatc
     9481 tgatttcaga atcaggctga tcggttatca gcgtcacatt tacagggaga gcggcaggta
     9541 actgacaaaa tttatccgtt atctccctta gcaagacacg taaagaggct tcaaccttat
     9601 gcccactgtc agaaagataa tctattttct ttacaaggcc gtactgcaga ggcaattcat
     9661 cacaaagagt agctaccgtt attttatcag gcaaataaac acaactggca gtaataacaa
     9721 gatacctttc ggcccaggat tcccactgtt tctgagcata ctccccctct tttttaagaa
     9781 acaaatgttc atccatctgg ttgctccact gccagtgtcg caggcaaaac aggaaaaacc
     9841 agcaccccga aggagtcaat gcaagccacc agatactgaa atcatttaat actttcacat
     9901 atcctgatgc ccggataata aacaacagag taccaacaac agccataaat gcccctgtca
     9961 taaaccacaa catcattgat ggtttatgag gtaattctgt tgataaagat tttgttctgc
    10021 tccatcccat ggtttatcct attgcacatt ccggtgcact ggatataacc ttacacccac
    10081 acagacattt acatccatca atcacaacag ctcttccatc agatatccat tcaggagagc
    10141 cctccacaat aggattggta ccatgtccgg aaacagggca actaaccata tctccaacaa
    10201 gagctacttt tttaccatcg acaatcatgg ttgaagatgc cgaaataact ttaccaccat
    10261 gtgtggtatt atcaccaagc aatacaaaac ctttagacat aaaactctca aaaataaatt
    10321 ttcactttga gtcaccagtt accgtaagga taagttttat ctttgataaa ttccttaata
    10381 tcttcatcat atccatatgt gaaatcactt ttagtaaccc ccttacacat attcatttta
    10441 tcacccgata ctgtagtaat tttagcatcc aactgaggta ccaccggatt accgcgatct
    10501 cccagccaga ctcttacctt cccttcaggt gccagaccaa taatcatcgt tgaacgataa
    10561 taggtttcgt tccagcggtt gtctgtgtaa ggcgtaacca tctgctccca ggttcctttt
    10621 ttaaaaaaaa tcagagtttc ataactcttt ttatcaatta tcgaatccca gcaaaaacgc
    10681 aacatcagag gtaatgcttt ccccttgtta taatacgact cagtcgcgcc cagatgctca
//
LOCUS       DAZGCQ010000147        10604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000147 DAZGCQ010000000
VERSION     DAZGCQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.147"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            40..894
                     /gene="rclR"
                     /locus_tag="ACHK07_004503"
     CDS             40..894
                     /gene="rclR"
                     /locus_tag="ACHK07_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO8104748.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQFAP"
     gene            1421..2140
                     /locus_tag="ACHK07_004504"
     CDS             1421..2140
                     /locus_tag="ACHK07_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8104749.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            2151..3578
                     /locus_tag="ACHK07_004505"
     CDS             2151..3578
                     /locus_tag="ACHK07_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8104750.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLIAMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            3571..4266
                     /locus_tag="ACHK07_004506"
     CDS             3571..4266
                     /locus_tag="ACHK07_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8104751.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(4509..5177)
                     /gene="ykgH"
                     /locus_tag="ACHK07_004507"
     CDS             complement(4509..5177)
                     /gene="ykgH"
                     /locus_tag="ACHK07_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO8104752.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIINWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5359..7656)
                     /gene="hyxB"
                     /locus_tag="ACHK07_004508"
     CDS             complement(5359..7656)
                     /gene="hyxB"
                     /locus_tag="ACHK07_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO8104753.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LDQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGAKGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSLLNSFEGDATDNITLAGSNIINGRIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGTGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYTFNSGDKAGSNLDINLSDSSVWKGKVS
                     GAGNASVSLQNESVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWEDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
     gene            complement(7698..8459)
                     /gene="hyxA"
                     /locus_tag="ACHK07_004509"
     CDS             complement(7698..8459)
                     /gene="hyxA"
                     /locus_tag="ACHK07_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO8104754.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVCNITYIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(8613..9407)
                     /gene="hyxR"
                     /locus_tag="ACHK07_004510"
     CDS             complement(8613..9407)
                     /gene="hyxR"
                     /locus_tag="ACHK07_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO8104755.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(9737..10300)
                     /gene="ipbA"
                     /locus_tag="ACHK07_004511"
                     /gene_synonym="fimX"
     CDS             complement(9737..10300)
                     /gene="ipbA"
                     /locus_tag="ACHK07_004511"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO8104756.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         2800 a         2364 c         2493 g         2947 t
ORIGIN      
        1 tgttggtgtt tgtaatcaaa aaccactcag gagtctgata tggatgccct tagccgtttg
       61 ttgatgctta acgctccaca aggaacgatc gataagaatt gcgtgttagg aagtgactgg
      121 cagcttccac atggtgccgg ggaattgtcg gttattcgtt ggcatgcgtt aacgcaagga
      181 gcggcgaagc tggaaatgcc gacgggagag atttttacat tacgcccggg aaatgtggtc
      241 ctgctaccac aaaattcagc tcatcgcctg agtcatgtcg ataatgaatc gacctgtatt
      301 gtctgcggca ctcttcggct gcaacattcg gcgcgttatt ttttaacgtc tttgccggaa
      361 acgctgtttt tagcaccggt taaccacagc gtggaatata actggctgag ggaggcgatc
      421 ccgtttttac aacaggaatc cagatcggca atgccgggaa tggatgctct gtgtagccag
      481 atctgcgcta cattctttac cctcgcggtg cgtgagtgga ttgcacaggt taatactgag
      541 aaaaacattc tcagtttgct tctgcatcca cgtcttggtg cggtaataca gcaaatgctg
      601 gaaatgccag gacacgcctg gaccgtcgaa tcgctggcca gcatcgccca catgtcccgg
      661 gcaagttttg cccagctttt ccgtgatgtt tccggaacca cgccgctggc tgtattaaca
      721 aagttgcgtc tacaaatagc ggcccagatg ttttcccggg aaatgctccc tgttgtggtg
      781 atcgctgagt cagtaggcta tgccagtgaa tcatcttttc acaaggcgtt tgtccgcgag
      841 tttggttgta ctccgggaga atatcgggaa agggtcagac agtttgcacc ctgaataaaa
      901 ccgccagaaa tcagggcaaa gataatccgc attccgggag ttgtgagcct ttcaactatt
      961 tctatttcca acggtggttt gggctttata tatttttttc tgatggactc tagctcagtt
     1021 tttaaataaa aatgcaaact aaattgcttc aattaaataa aatcccgact attacttgat
     1081 acgtgattat tgtcggtgat ttttttgtac accatcagtg tatatctcaa taacccctga
     1141 ataagtagct ctgaataggt ataagggatg tagccatttt taaatggatt tcttatgcca
     1201 aaaatgatcg tcgatttccc cataaaatgt gagcgatgcc gaaagaaata aaattagtta
     1261 ttgcatttga agtttggatg aaagattatc atttgtcata caaatgaggg gtggtatgtt
     1321 gctagccaat taaaaaagaa cgccatattt attgatgatt gatcccccgg tggggggaaa
     1381 catcgggcat aaatgggcat gaagtaatgg agtattagtt gtgaatgtca atttctttgt
     1441 cacctgtatt ggtgacgccc tgaaatcaag aatggcacga gactccgtgc tgctactgga
     1501 aaaactcggc tgtcgcgtaa atttcccgga gaaacaggga tgctgcggtc agcctgcgat
     1561 caatagcggt tatatcaaag aagcgattcc agggatgaaa aatctgatcg ccgcactgga
     1621 ggataacgat gatcccatta tttcaccggc tggctcctgc acctatgccg taaaaagtta
     1681 cccgatgtat ctggcggatg aacctgaatg ggcatcacgt gccgcaaagg ttgccgcgcg
     1741 tatgcaggat ctcacctctt ttattgttaa taaattaggg gtagtcgatg taggtgccag
     1801 tttgcaaggg agagcggtgt atcacccatc ttgtagcctg gcccgtaagc tgggagtgaa
     1861 ggacgagcca cttacgctgc tgaaaaatgt gcgtggactg gagctgttga cctttgctga
     1921 acaggatacc tgctgcggat ttggcggcac gttctcggtc aaaatggccg aaatatccgg
     1981 cgagatggtg aaagaaaagg ttgcgcacct gatggaagtc cgccctgagt atttaattgg
     2041 tgctgacgtg agttgcctgc taaacatcag tgggcgatta caacgggaag ggcagaaagt
     2101 caaagtgatg catattgctg aagtgttgat gagccgctga ggatataaag atgtcgatca
     2161 aaaccagtaa tacagatttt aagacacgca tccgtcagca aattgaagat ccgatcatgc
     2221 gcaaagcggt ggcaaacgcg cagcagcgta ttggggcaaa tcggcaaaaa atggtcgatg
     2281 aattggggca ctgggaggag tggcgcgatc gggccgccca gatacgtgat catgttctga
     2341 gtaatctcga cgcttatctg taccagctct cagaaaaagt gacgcaaaac ggcggtcacg
     2401 tctattttgc aaaaaccaaa gaagacgcta cccgctacat tttacaggtt gcccaacgca
     2461 aaaatgcccg gaaggtggtg aaatctaaat cgatggtgac cgaagagatt ggtgtcaatc
     2521 atgtgttgca ggatgctggc attcaggtga ttgaaaccga tctgggtgaa tatattctcc
     2581 agctggatca agatccgcca tctcatgttg tggtcccggc aattcataaa gatcgccatc
     2641 agatccgtcg agtgctccac gaacgtctgg gctatgaggg gccggaaacg cctgaagcga
     2701 tgaccttatt catccggcaa aaaatccgcg aagatttcct cagtgctgaa ataggtatta
     2761 ccggctgtaa tttcgcggtg gcagagacag gttcggtatg cctggtgacc aatgaaggta
     2821 atgcgcgaat gtgtaccacg ctgcctaaaa cgcatattgc agtgatggga atggagcgta
     2881 ttgcccccac gtttgccgag gtagatgtat tgatcgccat gctggcgcgc agtgccgttg
     2941 gtgcacgttt gacgggatac aacacctggc tgacaggacc gcgcgaagcg gggcacgttg
     3001 atggtcctga agagtttcat ctggttattg tcgataacgg gcgttctgag gtgctggcct
     3061 ctgaatttcg ggatgtgctg cgctgtattc gctgcggggc ttgtatgaat acttgtccgg
     3121 catatcgcca tattggcggt catggatatg gctctattta tccagggcca attggtgcgg
     3181 tgatttctcc gctacttggc ggctataaag attttaaaga tttaccctac gcctgctctt
     3241 tatgcactgc ttgtgacagc gtgtgtccgg tgcgtattcc gctgtcaaaa ctgattttgc
     3301 gtcatcgtcg ggtgatggct gaaaaaggga tcaccgcaaa agcagagcaa cgggcgataa
     3361 aaatgttcgc ttatgccaat agtcatccag gattgtggaa agtcgggatg atggccggcg
     3421 ctcatgcggc aagctggttt atcaatggcg gcaaaacacc actcaaattt ggcgcgatta
     3481 gcgactggat ggaagcacgc gatcttcctg aagctgacgg agagagtttc cgtagttggt
     3541 ttaagaaaca tcaggcgcag gagaaaaaga atggataatc gaggcgaatt tttgaataac
     3601 gttgctcagg cactgggtcg cccgttgcga cttgaaccgc aagcagaaga tgcgccgctt
     3661 aacaactatg ctaacgagcg gcttacccaa cttaaccaac agcagcgctg tgacgcgttt
     3721 attcagtttg ccagcgatgt tatgttgacg cgctgtgagc tgaccagcga ggcgaaggcg
     3781 gcagaagctg caatacgtct gtgtaaagag ctgggagatc agtcggtcgt gattagcggt
     3841 gacacgaggc tggaggaatt ggggattagc gaacgtttgc agcaggaatg caatgccgtt
     3901 gtttgggatc cggcgaaagg tgccgagaat atctcgcagg cagagcaggc taaagtgggt
     3961 gtcgtgtatg ctgaatatgg tttaaccgaa tcgggaggcg tggttctttt ttccgccgcc
     4021 gagcgcgggc gttcattgag cctgctcccg gaatcttctc tttttatcct gcgtaaaagc
     4081 acaatactgc cgcgtgtagc gcaactcgca gaaaaattgc atcagaaagc gcaggccggt
     4141 gaacgcatgc cgtcctgcat taacatcatt agcggcccta gttcaacggc ggatattgag
     4201 cttatcaaag tcgtcggggt tcatggcccg gtgaaagcgg tgtatctgat tattgaggat
     4261 tgctgagaga gatatgaagt ctggacataa acctgaaggc ggcatacgag gttgtgaata
     4321 gcgttaagga caacctgact gaaccccgtt ccgtcatggt tatgctcctc ttctgtaaat
     4381 atcctggcat tagtaatgcc aggatattta cagtgaaaaa gacaggcatc cattcctgaa
     4441 aacgggttcc cttcccggtc attgtcactt cagctttata caggcactct ctatcagaat
     4501 gctgtttatt actgcccaga gttttgttgg tattcatcta ccattttttt gacaaggcaa
     4561 aacattacag aaatcacaat gcttagaaaa aatcccagaa tcaggcaaat ggcagcaggg
     4621 tatttatttt ttatttctgg tgagcttata gttacacagc catcaccgtt cggatacaat
     4681 gaccagttcc tggtcaggta tatcattttt gtataaagct cactgttaat tgctgaaggt
     4741 gtttgtatct catcgagaac ttgcctgcca ttatggtatt ctgacgcata ccaattgata
     4801 atagacgcct gcacgcgaga tatttccgct tcagcagttg gtaaatctct cttatctcct
     4861 gaccattcca gctttatttc tctgttcttg acatctgggc tggtgtgaaa aaatgtagta
     4921 attcgctgaa taacatcttc agtcattgtg gtctgacagg caaagttatt attgctatca
     4981 cagttaacaa gtatgcctgg tgttgtctga tttattttta ggctgtaact cacttttata
     5041 ttatctttat tgatatacag aaacaacagc accattgcca tacagatagc tataataaaa
     5101 aggataacac gaatcttttt cttcagataa aataaaatct caattagatc gatatcctgt
     5161 ttgatttgtt cactcataat atatccagaa aataaaatct gccgcagata aatgaattaa
     5221 tagtctgtat caggaatgtt cgggttaaat atcagcaaaa agccagcatc atgaatactg
     5281 gatatttata gcctgcaggc agcatactgc tgcctgcagg gggtataaca gtcgacaggg
     5341 gaaccgactg ctttattctt accaggtcac attgatacca actacaccct gtagcggatt
     5401 ctcaatgtcg tcacctttgg tgtagtcaag gcttgcatat gctcccatgt ttttggtgat
     5461 atcagcctgt acacctgcac ccacgcggaa tgctgcaccg tcaatgctgg cattgacaga
     5521 ctcatcaccc agacggactt tgttgccatc agagaattcg ttcagtgcgg ccagattcag
     5581 ataaggttta acggtcgcgt tgcccacagc gaagtcataa cccacgcgcg taccggcctc
     5641 acccagcaca gagttatagc tgtcagaatg aacgttcacg ccgttgagtt gataatccac
     5701 accttccagc gtcagcgcag acagtttggc atatggctga ataaacaggc cattaccgtc
     5761 ttcccaggtg taaccgcctt taaccatcgc accgaaacca ttggtgttaa agtcagcttt
     5821 gatggtaccg ccaccattca tcaggcgaac atctgccgtg ttgctgtagt gaccaaactg
     5881 tgcagcagta tcaatgaaga taccgttgtt atactggcgg ctcaggtaag cgtggaagct
     5941 gtaaccttca gtgtcgcctt tgctctgcat ggtggtcatg tcacctttcg cagaagacat
     6001 cgccacaccg gccagccagc taccatcttc agtcatgaag cgggtatcac cacccagcat
     6061 tacaccgttt acatccaggt cataggatgc atttcccgcg gtggtatgtt tctgtttacc
     6121 gccaaagtac tgaatccata cgccgccctt gtcattttcg ctcagacgga cagcatcctg
     6181 acgggctgag agcgtgtcag cctgcagatt agcaacgtta gccggagctg ctgccattgc
     6241 ctgaatcacg gagctggcgt tggatgcttt atgcgtgttt tttacgaaat aaaagttacc
     6301 atcagctccc tgtgtcaggt catattgata cagaccacct tccgttgtgc ctgtggcttt
     6361 tagagtgctg tctttgccgg tagaaataaa tgccgtttgt tcattgataa gatccaatga
     6421 acccgcactg taaacacgca agtcaccgct cgctttaccg ctgatatcca gagtgttttc
     6481 agaagaggca tcaacaatca gagtgccgtt ttgagaagca aacgtgccag tattgactgt
     6541 agccttcgtg atattaaccg tactgtcttt tactgccaga gcatcaacag ttgaggagcc
     6601 cgtaacattc cagacagact cgttttgcag agatacactg gcatttcctg cccctgaaac
     6661 tttgcctttc cagactgaac tatcggaaag atttatatcc agattactac ccgctttatc
     6721 gccgctattg aaagtataag cattaccttc ccagtaagag cgggcaacgt tcatgtttac
     6781 atttgttgtg gtagcggctg aattctcgct ttcagaaagg gccaggacat cagagtagat
     6841 ggttgaatca gcaacagaca aatttaaatt ttgcgttgct gaacctgtgc cttgtgccga
     6901 agcagaggca taaattgttt gtttattatt agccgcccca attactgagc catccttaat
     6961 attcaggttg actgtatgaa ttcccgtctt attcccggca gtaactattg tttcaatccg
     7021 accattaata atatttgagc cagcaagggt aatgttgtcg gttgcatcac cttcaaaact
     7081 attcaataag ctaccctttt gagcaaaaat tgccgtggag gcgttgctgg aactcttatt
     7141 accttgagca tttatggttg tgccattaag cgtgacatta tttcccttgg ccccttccag
     7201 agatatccct gtcgcactac ctttttcatt ttttgcaacg aaattaccgc cattaacaac
     7261 aacatcgcca gttgaatcaa tgaccattgc agtccggaca taggtgcctt caccttgagc
     7321 tgtgatggtg gagtctgtag cattaatcac aacatttttg ttaccactaa cttttacacc
     7381 ttcaacgaaa tcttggttta ccgttgatgc ggcggtaata tcatgaccac caaaagtaac
     7441 attaaggtct ttatctgaat ctgacgtttc aattgttacg gctgtaatct ttttatcacc
     7501 gttaataaca acatcttgat ccagagttgt cttatcttta tccaatgtaa ccggtgtagt
     7561 tgataccgcg ttatcagcag cgaatgctgg tgcagaaaga gccataacaa tagctgtcgc
     7621 taatgcgctg cggtaaaatg ccttactgtt tttcatgaag aaattctcca ttctggtaac
     7681 taatttcaat ttcccactta tttatattta tgcagagata aagcctgcgt ccctgcattt
     7741 accaccacgg aatacctgtt ggtatttccg ttttcacgca atgtcatggc attcgttttt
     7801 tctaatgcat ttccatcacc gtgaaattta ttgctgctca gcagcagggc ttcccgcaat
     7861 gacatgctgt ccggtattcc ccaacgcttt tttagcgact gctcggcaag ccatatctgt
     7921 cttatccccg ttttgagaaa ctgcgcactg tattcccaac tatgtccggc aagaagcaac
     7981 gacaacactt tcttttgctt ttccgggaag cgttctgaca tgacgattgt ctttgtgcgg
     8041 ttattcacct cattcataaa gacagagtaa ctgtccataa ctggcaaatt ttctgcatgt
     8101 ttcagtcctg aaaacccacc agaggcattt gcaatactga tgacacgctc tactgacatt
     8161 cctggtttca gtaacctcag gtttttgaga acgccagaaa tactcacatc cggtggcaga
     8221 aaaaagggcg cgacgcacaa caccaccacg aatgagtgat cccgataaaa tcggaaccac
     8281 atttcagcgc attcggcagg tcgccctgcc gatatcacga cactctgtgg cctgtgccgc
     8341 aatttattgc gtaactcctc ttctgttctg atgtaggtaa tattgcagac agaaccggtt
     8401 gatagagctg cgaactgtgc aggtacaatt cctgagggac agaaaagaac gaaacgcatc
     8461 cttccctcac agatgtcagt acgccagcac gctgtacagg ctgtacagcg tgcctcccaa
     8521 tcctgcagcc cagagcagta gaaatatttt ggcccctaca ctgtagcgag tccgtacgcg
     8581 ccgcagtggg gggtagtaac aacgctgctg tgtcataaca atcctggtct ttttttagac
     8641 atattttttc tttgtccgga aagtactttt ccggatatgg cgtcagaatt tattgattca
     8701 ccaacggcaa ctttctctgg ataaagaatt ccggatacca tgttctgcac atactgagtg
     8761 agtcgatgag cggaatgttg tccgacatat tttgcatgta gccgtatatt ccgtttcact
     8821 ctgctgtcac cgcatcccgt atagagcagc ctgaaatcgc tcagagttcc cagtccaagt
     8881 ttcattaatg cccgtgtggc aatcgcatgc agggtctttg aactgcgttg ttcataacgg
     8941 gcaatctcac tgaaactcat gccctgcatc agaatcttaa gcacgctcaa ctcccgtgca
     9001 gtcaggagcc agaaatcgct catatcgccg ggcaaaggac gtagtaacca gcggataatt
     9061 tgattctgat aacgactcgc tgtcagcatg gtgccgatgc gtgggaagcc gttatacaga
     9121 cttgcagcag gagcattatc gaaccagtcg gagtcaccgg tcaggcagac ctgccattgc
     9181 ccggcatcac tccgcaagcg gacaagacaa gccagaagaa ctaactcccc cgaaggaagc
     9241 gaagggatat cagccaacag atagccacag gaggtcttta aaacgaattc aacctctgta
     9301 gtgacgttgc tgtacacccg tacgatatac ctgtccgctg ggatgagaga ctccagtgcc
     9361 agcctggtga actgacaggc actgataata accagaggct tcttcattca gactcgtcca
     9421 cgccattgct acaaaacctg acattttgat tcaaaatgtc aggtttggtt taattgcctg
     9481 aatatttaat tttcttggaa tataaatcta atttgtttga tttttattgt ttttaatttt
     9541 gtgttattta acaatatgag cgaaagttaa tcactggtat gacctttgtg gttaatcatt
     9601 tcaaatcatg gggttatttt actttagcga gaaatatcaa catgttatgt gttttattta
     9661 attgaatggg ttgttttttt atggaagaat tgaggaaaac gtaggaatct gacattttga
     9721 atcagaaggt actgttttaa cgaggctttt ttttccacac ccctttaaat cgagcagcat
     9781 tactggctgt gtatctaaca gtatgctgga tatttcgatg acccagataa tcctgaagta
     9841 gtcgggtatc gacaccattg tcagccagag caaaaccaca ggcatgacgt aacatatgag
     9901 gatgtgcttt tacagctaat cctgcacgtt ttccagcctc acgaatgata gaaaaaaaat
     9961 gttgtctgct gatagggcgg cgtttccggg agagaaaaag ccagtctcct tcaactccgt
    10021 tttcaattaa cttcctctgc ttaagccaca acttgatcaa attatattca tcaggaagaa
    10081 gaggatgtgt cgtcgagaaa ccatttttaa tacgccgaat attaagttgt tttcctgatg
    10141 catcaatatc ggataaacgt aaatcaagaa gttcactggc cctaaaaccg tgaatgaaag
    10201 ccatcataat tagacagcga tttctttcag gaaaggacat gctctgggct gcatccatca
    10261 gcctgtagac ttcatcctgt gtgagatatt ttctcgtcat acgccaccgt gtacagttgt
    10321 aaaaaagaga ggcacaccca gataagtatg cctgccatca gaacgttatc gatgtaatct
    10381 cattatctca ttaataagat tttttgaata gtagttctac gcaatagact aaatatgaat
    10441 tatttattgt gcagcttttc tacaagccat ttttaatgcg tgtttttatc tacagcgatg
    10501 tggaaaaaat aaatataaaa gctatgaatt acagggcgtg ggggatgtgt gttacgtcta
    10561 atcttttagt gatgtttagt agttttgaga taattgacgt tatt
//
LOCUS       DAZGCQ010000148        10597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000148 DAZGCQ010000000
VERSION     DAZGCQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.148"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..3316
                     /gene="yjiT"
                     /locus_tag="ACHK07_004512"
     CDS             26..3316
                     /gene="yjiT"
                     /locus_tag="ACHK07_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjiT"
                     /protein_id="HFO8104757.1"
                     /translation="MGQSEYISWVKCTSWLSNFVNLRGLRKPDGRPLYEYHATNDEYN
                     QLTQLLRAVGQSQSNICNKDFAACFVLFCSEWYRRDYERQCGWTWDPIYKKIGISFTA
                     TELGTIVPKGMDDYWLRPIRFYESERRNFLGTLFSEGGLPFRLLKESDSRFLAVFSRI
                     LGQYEQAKQSGFSALSLARAVIEKSALPTVFSEDTSVELISHMADNLNSLVLTHNLIN
                     HKEPVQQLDKVHPTWRSEFPIPLDDETGTHFLNGLLCAASVEAKPRLQKNKSTRCQFY
                     WSEKHPDELRAIVSLPDEVSFPVTSEPSTTRFELAICEDGEEVSGLGPAYASLENRQA
                     TVRLRKSEVRFVRQNPSAGLSLVARAGGMIVGSIKLDDSEIAIGEVPLTFIVDADQWL
                     LQGQASCSVRSSDVLIVLPRDNSTVAGFDGQSRAVNVLGLKALPVKGCQDVTVTANET
                     YRIRTGREQISIGRFALNGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGIS
                     IEQCHIQEMLGAQYLSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQH
                     PCVATLKDKTLEATRKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADTIDIELPFPAK
                     GCLALDVNGRPLDKNITLHDLLGSRAFLFSRNGEPTKYTLQLHLRSISGLQAWHEWSY
                     TALSERPVELNLYSLREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYD
                     YEKELLLSQSVNHRAGQIPSPVIMLLSEPERKSIPLVSRMSEGVPVGEYELSSIVNKN
                     GPWLVVPKPGEEMAFRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNAITLVLGQ
                     MANDPAHSGWQFMRSLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRI
                     ENEFPILWEFFPIFEIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADE
                     IEKWLSNGYLPPSIPESCVHGWYQELLREHSEARWPEYGCKRLYKWMMSQKNPVIDIN
                     PDANHRYSVAWLPVFAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQCS
                     LLRYVAKK"
     gene            3329..9673
                     /locus_tag="ACHK07_004513"
     CDS             3329..9673
                     /locus_tag="ACHK07_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8104758.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPKLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDDQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTENLHASVKSEQAKRPNEVLSR
                     EKMREEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIAGSDAEKQLKKFLSELSGIAQERIDVLDGS
                     RVIPELEPCKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVSQPNPMKLDEMTQRLNALTKQHYSQQEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKNCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLAQDKKGKLVQWENKGGDEFSLQDEVPVEHDATEEKVEKENSFQPPLIIAAGETSEA
                     GYTLQRLDRQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGIGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSLLRGSVVEILSWHQRTQTSTAPNANADLEKLAARAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQAATGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNHYLQPEV
                     FNKNGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYQGLEKIHKSPLHWQEKGLT
                     LDDWRDFLKVTLDLYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVNNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKVWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTINAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKSQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKITMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QNGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISAAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     VALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAIQSYRLKDNVEVDLVIPAGVDDPDLLHELSQFTALGVRLCHVEQEL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYQTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMKNTRITGVHYTDRYLQ
                     NPVALALLGSILKPLKTKLTDGAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACKEGNVANSEESWATDVLVEVTAS"
     gene            complement(9716..10057)
                     /gene="symE"
                     /locus_tag="ACHK07_004514"
     CDS             complement(9716..10057)
                     /gene="symE"
                     /locus_tag="ACHK07_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO8104759.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVIAGKQKVA"
     gene            complement(10285..>10597)
                     /locus_tag="ACHK07_004515"
     CDS             complement(10285..>10597)
                     /locus_tag="ACHK07_004515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010671752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8104760.1"
                     /translation="LGILKELPIPLPSLEEQREIVRRVEQLFAYADTIEKQVNNALAR
                     VNNLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKASRKK
                     S"
BASE COUNT         2742 a         2456 c         2767 g         2632 t
ORIGIN      
        1 atcttttatc tctaaggagc ttgctttggg tcaatcagaa tacatttcat gggtaaaatg
       61 tacttcctgg ctaagtaact ttgtgaatct tagagggttg agaaaaccgg atggccgtcc
      121 tctttatgaa tatcatgcaa ccaatgatga atataaccaa ttaacgcagc tactccgtgc
      181 agtcggtcaa tcacaatcta atatatgtaa taaggacttt gctgcctgtt ttgttctttt
      241 ttgctctgag tggtaccgca gggattatga acggcaatgc ggatggacgt gggatcctat
      301 atataaaaaa atagggattt catttaccgc cacagagctt ggtactatcg taccgaaagg
      361 gatggacgat tactggctgc gcccaattcg attttatgaa tctgaacgac gcaattttct
      421 cggaacatta ttcagtgaag gtggtttacc tttccggtta ctcaaagaat cagacagtcg
      481 ctttctggct gttttctcca ggatccttgg acaatatgag caagcaaagc agtcaggttt
      541 ttctgccctt tcgctggcaa gagccgtcat agaaaaatca gcgctgccaa ctgtttttag
      601 cgaagatacc tctgtagaac ttatcagtca tatggctgat aacctcaatt ccttagtact
      661 tactcacaac ctaataaatc acaaagaacc agtacaacag cttgataagg ttcaccctac
      721 ctggcgaagc gagtttccaa taccgttaga tgatgaaaca ggaacgcatt ttttgaatgg
      781 actgctatgc gcagcctctg tagaggctaa gcctcgacta caaaaaaata agagtacgcg
      841 ttgccaattc tattggtcag aaaagcatcc agatgagctt agggcgatag tatctctccc
      901 tgacgaagtt tcctttcctg taacaagcga gccgtcaact acgcgctttg aacttgccat
      961 ttgtgaagat ggtgaggaag tctctggcct tgggccagcc tatgcttccc tggaaaacag
     1021 acaggcaaca gttcgattac gtaaaagcga agtgagattt gtcaggcaaa atccatcggc
     1081 aggtttgtcg ttagtggctc gtgctggagg gatgattgtt gggagcatta aacttgatga
     1141 cagtgaaatt gccattggtg aggtgccgtt aaccttcatc gttgatgcag atcaatggct
     1201 gttacaggga caggcctcct gcagtgtgcg aagcagcgat gttctgattg tgctccctcg
     1261 ggacaatagc actgttgctg gttttgatgg ccaatcgaga gcagtaaacg tattaggact
     1321 taaagcgcta cctgttaagg gatgtcagga cgttacagtt acagcaaatg aaacttatcg
     1381 gattcgcacc ggaagagaac aaatcagcat cggaaggttt gctctaaatg gaaagcgtgc
     1441 aagctgggtt tgtcatccag atgagacatt tattggtgta ccaaaagtca tttctacact
     1501 accagatatt caaagcattg atgtaacgcg ttacctgagc ggaatttcaa ttgagcaatg
     1561 tcatatacaa gagatgctag gggctcaata tctttctatc cgtaatagta ataatgaaac
     1621 gctcttgcgt cgcaaaatcg gtatcttgcc tgctgatttc agtattgaga taaaaggtgg
     1681 aatgcacgca aatgaaggca ctatcgtaat caccacacag catccttgtg tagcaacact
     1741 taaagataaa acattggagg ctacacgtaa gcgtactgag gggcgaacag agatacaact
     1801 caaagcggaa ggggtacctc cggcatttat tacattacaa gttctgccta atttggcggc
     1861 tgataccatc gacattgagt tacctttccc agctaaaggc tgccttgctc ttgatgtaaa
     1921 cggacgtcct ctggataaaa acattacgct gcatgatctg ctgggttcca gagcattcct
     1981 gttctccaga aatggtgagc cgacaaaata tacattgcag ttgcatttgc gctccattag
     2041 tggattacag gcatggcacg aatggagtta tacggcattg agcgagcgtc ctgttgaatt
     2101 gaatctctat agtctgcgcg aacatattga gaacctgatt tcacttgagg caggtattga
     2161 tcaggttgtt gagatgcgta ttacgggggc gggtgtggtc atggcctggc aaattcgtcg
     2221 ctataaatac agcctgcgct atgattatga aaaagaacta ttactcagcc agtcagtgaa
     2281 ccatcgtgct gggcagattc cttcccccgt tatcatgctg ttaagtgaac ccgagaggaa
     2341 atccattccc ttagtatcta gaatgagcga gggagtgcct gtcggtgaat atgagttgag
     2401 ctcaatcgtt aacaaaaatg gaccatggtt ggttgttcct aaaccgggtg aagagatggc
     2461 ttttcgtccc tgcttcattc gtggggaatc ttcgttaccg gtagaagaaa gcaacatccg
     2521 ctcattgcag aaagcgaccc agctttttaa tccacaggca gaagttaatg ccatcaccct
     2581 ggtgctcgga cagatggcta acgatcctgc ccattccggc tggcaattta tgcgtagcct
     2641 gtacgatcaa tttggttatt taccgctggc aacctttgaa gtatggcgtg cactggtgca
     2701 gcatccgcag gcattggcaa tgtcgctctt caaatttgaa atgtcagcag agtacctgag
     2761 tcgtattgag aatgagttcc ctattttatg ggagtttttc cccatctttg agatcaaagc
     2821 tgcatctgag cgttttaaat tgttcctgag ccaaaaaggt gcaccggagg aaactcagaa
     2881 gttactggtt actaatatgt ttcaacggct ggggctcgtg ttcccgacat atgccgatga
     2941 aattgagaaa tggttaagta atggatatct ccccccgtct attcctgaat cttgtgtcca
     3001 cgggtggtat caggagctct tacgtgagca cagtgaggct cgctggcctg aatatggctg
     3061 caagcgtctg tataaatgga tgatgtcaca aaaaaatcca gtgattgata ttaatcctga
     3121 cgcaaatcac cgctattccg tggcctggtt acccgtcttc gccgcagcgg tggcaagcgg
     3181 aaatgcttct tttgaatctg tatttgaccg taaacccggt gccgtatttt tcctgaggca
     3241 agtcagggat ttcgattccc gctggtttaa ggccatcttc cagtgcagcc tgttgcgtta
     3301 tgttgctaaa aaataaaaag gaataaggat gactacacgc tatttttcgt cacttattga
     3361 acaatcgttg tctcgttcaa ctgaagccac gctcagtatc atgggcgtta caaacccgaa
     3421 gttgcgtgaa catctcgcgc agcaaatggg ggctgattgt gggaaaccgg gctcattcct
     3481 tgcttctccg gtattccagc aaatgtttgg ctggaaggag tcaaaccaca cgatgcgcag
     3541 cctgacggaa ggcaacgcat tgttgagcaa agcagtggtt gattcactgg atgatcagag
     3601 taatggccgg taccggtttg gtgctgactg gaagcctttt acgcaccagt tagccagttg
     3661 gaaagctttg ctggaaaaga aacattcggt ggtggtaacc agtggtacgg gttcaggtaa
     3721 aacggaatgt tttatggttc cggtgctgga agatctttac cgggaactgc atgaaaatgg
     3781 caacaatccg ttggtcggtg tccgagccct ttttctctat ccattgaacg ccctgattaa
     3841 ttcacaacgc gagcgtctgg atgcctggac gcgtggtttt ggtaccggga tccgctactg
     3901 cctttacaac ggcaacaccg aaaacctaca cgcttcggta aaaagcgaac aggcaaaaag
     3961 gccgaatgag gtgctctcgc gcgaaaaaat gcgtgaagaa cctgcgccga ttctggtgac
     4021 caacggcacg atgctggaat atatgatggt tcgccaggtc gacgccccga ttatccagca
     4081 atccaaagca caaaaatcac tacgctggat tgtactggat gaagcgcata cctatgtcgg
     4141 gtcgcaggcc gctgaactgg cgctccagct gcgtcgtgtg atgacagcgt ttggcgtgac
     4201 gccagatgac gtgcgctttg tggcgacctc tgcgaccatt gccggcagcg acgctgaaaa
     4261 gcagctgaaa aaattcctct ctgaattatc cggcattgcg caggagcgca tcgatgttct
     4321 ggacggaagt cgggtgattc ctgaactgga accatgcaaa aacgtttctg ttccgttaga
     4381 cgaaatagag caaatacctg atctggatct aaaagataaa aatgataata aaattaaagg
     4441 gataagtcct gagcgctttt atgcgcttac gcactccccc gaagcgcgtt acatacgtga
     4501 aatgttggtt agccaaccca atccaatgaa actggatgag atgacgcagc ggttaaatgc
     4561 gctgacaaaa cagcattaca gccagcagga agtactgcgt tggatcgatg tctgttcagg
     4621 gactcaacct aatgcaaagg atcccgcatt tcttaaagtc agggcgcata tcttccagcg
     4681 taatacccag ggaatatggg cttgtgtgga taaaaattgc agattaaagc acggtacacc
     4741 gctcgaaaaa ggctggccct ttggctatgt gtatgtgaac cagcgacaaa attgcgactg
     4801 tggaagccct gtctacgaag tggcgttctg taatgattgt aatgagcctc atcttctggc
     4861 gcaggacaaa aagggcaaac ttgtccaatg ggaaaataaa ggtggcgatg agttctcctt
     4921 gcaggatgaa gtacctgttg aacatgacgc tacagaagaa aaagtcgaaa aagagaacag
     4981 tttccagcct ccgttgatta ttgccgcagg agagaccagc gaggccggtt ataccctaca
     5041 acgcctcgac cgccagacgc gccgtattgg cgttattaac aatgacagca ttccgctgat
     5101 tattaatgat gttgaacagg tttgtagcgc cagtggctgt ggctacagag gcatgagtgg
     5161 gaaacagccc ttccggcgtg cactattagg tgggccattt tacgttacta atatcgtgcc
     5221 caccgttctt gagtattgtc aggactttac cagtgatgaa ggcaaagagg gcatcggacc
     5281 agattcgttg ccagggcgag gacgtcgact cattaccttt acggacagtc gacaaggtac
     5341 agctcgaatg gcggtgcgta tgcagcaaga agcagaacgc agtctcttac gcggaagcgt
     5401 agtcgaaatt ctgagctggc atcaacggac gcaaacgtcc acggcgccga atgccaatgc
     5461 cgatctggaa aaattagcgg ccagggcgaa gcaagcccgt gagcaggcag aagaatatcg
     5521 aagctgggga atgccagacc aggcgaaatt gtcacaagcg caggctgaac agcttgaaca
     5581 ggcttatcag gctgcaaccg gtggaaaagc agcaactacc ctggtatccc gcacctggac
     5641 agaaatggtt aacgagctta aagataaagc cgatatccgt ggcccggtac tgaaatataa
     5701 ccactatttg cagcctgaag tgtttaataa aaacggcggg ccactaaagc tttcggaaat
     5761 gttgttgttc cgggaattca tgcgtcgccc taaacggact aacagcctgg aaacacaggg
     5821 gctggttcag gttggttacc aggggctgga gaaaatacat aagagcccct tgcactggca
     5881 ggaaaaagga ttaacgctgg atgactggcg cgattttctc aaggttacgt tggatcttta
     5941 tgttcgcgaa agcaacttca cacagctgga tgatgagctg aaaaactgga ttggtagccg
     6001 tttttcatca aaattcgtcc gtaacccgga atcaaaagat cctgaagata atcagaacag
     6061 acgctggcct caaattcgta atggcaacgt atcccatcgt ttagcgaagt tactgatgct
     6121 gggggctgga ttcaaaaccg tcaatacggc gactattgat attatcaata catggctgaa
     6181 agaagcatgg gcccaactta ccggaccact tgcagtactg aaacccgatg gcaaccgttt
     6241 ttatttaccg aaagagcata tgactttttc tttaatcacg gatgcctgga tttgcccggt
     6301 aaccaataaa atcctggata cggcttttaa aggcttaacc ccttatctgc ccacccatat
     6361 ttcgttcgag catcttaccc aggcgcaata tgacactttt gtcgcgcaga aagtcaccat
     6421 gccggaaatc tggaagctgg atcgttcaca ggaagactac gcagagggtc tggcaaaagc
     6481 acgagactgg gtcaacaatg atccgctgat cgcacaattg cggtcggaaa acgtctggac
     6541 agacatcaac gatcgtgtcg tggaaggcgg tttctactat cgcactgccg agcactctgc
     6601 ccagcagtcg tcagaacgcc tgcaaagcta tgaaaagatg ttcaagaacg ggcaactaaa
     6661 tgttctcaac tgttccacca ccatggaaat gggggttgat attgggggca ttactgcggt
     6721 ggtaatgaac aacgttccgc cacatccggc caactattta cagcgcgccg ggcgagccgg
     6781 gcgtagtaaa gaatcacgcg cgatctctta taccctatgc aaaggtaacc cacacgacca
     6841 gcaggttttt gccaacccac tctggccatt tgaaaccatg atcccggcac ccatggtcgc
     6901 gatgaactcc gcccgactgg tgcaacgtca cgtcaatgca ttattactgt ctgacttttt
     6961 atgtaacgtt attggcgaaa ccgataaaga gaaaaccagc ctcgatagcc tctggttttt
     7021 cggtgaagat gacgggcagt caaaatgcga acgctttaaa gtctggctgg aacgcccggt
     7081 tcttgatatc gacacggcgc tggaacggct ggtgaaaggt acggcgttgc atggcgcaag
     7141 agccgaacat ctgcgggata aaacaatcaa tgcgatcacc ttcctgcaac agcggtggct
     7201 ctccgtttat cgcgatctcg tgacccagga gcgtgagtcg cagccgcaaa cgccttatcg
     7261 taagcgcata gagctggaga agaaacggca ctgtggcgag tatctgctgc gcgatctggc
     7321 tgccagaacc ttcctgccgg gatacggttt cccgacagat gtagtgacgt ttgataactt
     7381 cacgatggaa gattatatcc gcgaaaaatc gcagaaaagt cgtgataaaa aggaccgtga
     7441 agataacgtc tcccgttata aaggattacc ttcccgtaac ctcggtgttg ccattcgtga
     7501 atatgcaccg ggagcagaga tcattcttga tggccgtgtc ttccgctctg ccggtgtctc
     7561 actgcactgg cacaacatta atgctgatac caatgaagcc cagcgtctgg attgcgcctg
     7621 gcgctgtcac aaatgcggga ccattggcta tgaagagggg atgagcagtt ccgggatgct
     7681 cttttgctcc aacagcgcct gcggcgaaaa aataaccatg gataatcgcc gtcaggtgtt
     7741 gcagcctgcc gggtttgtca ccgatgcaca cgctccggta acgaacaata ttgaaaccat
     7801 gaaattcgtc ccggttgtcc ccgcctgggt ctttgttaaa gcagagcccg taccgttgcc
     7861 taacccgcta atgggttata tggcttctgg ggctgatggg catgttttcc agcaaagtct
     7921 gggggaaggt ggacacggtt atgcattgtg tctgagctgt ggccgtgcgg agtcgatgct
     7981 gaatgaaaat gatgctccga aaagtatgga agctcactat cctccgcgtc cgggtaaagc
     8041 ggaccgcgac agccagaatc atcgtctgat ttgccccggt tcgacagcac tcatgaagaa
     8101 cgtcaccctg ggcgcactgg cgcgcaccga tgtctttgaa atggttctgc gcaaaccaca
     8161 aaatggcgag tatctgccag ataacacaga ggaggggcgg attgtcgcca tgactctggc
     8221 ggttgctctg cgtcaggcgc tggcaggcgt tctgggtatc tccgccgcag aactcggcta
     8281 ttctgtacgt ccggtcagac tggaggatgg gcaatcggta ttagccgttc agctttatga
     8341 tgtgatcagt ggtggtgcag gttttgcctc cagcgcaccg gtacacattg aagctatatt
     8401 gcagggaatg gtgaaacagt taggctgccg ccattgtgat actgcatgta gtgaatgcct
     8461 gcttgattcc cagactcgcc acgatcacga tttgctggat cgcaaagttg cgctggcctg
     8521 gcttggcgat gacttcacgt attacatcgg tttaccggat gaagagacat tctcgctgcc
     8581 ggatgcccgc tactgccctg gcgctattgg cgatacgatt cgccgggcga ttaacgaagg
     8641 tgctgaaaag cttaccctat ggatgaccgg cgcgcccaat gagtgggatt tatacgcccg
     8701 tcagttccgc gcagctattc agagctatcg gctaaaagac aacgtcgagg tcgatcttgt
     8761 tatccctgcg ggagtggatg acccggatct attgcatgaa ctttcgcaat ttactgcgct
     8821 gggtgtgcgg ttgtgccatg ttgagcagga gctgcaatta ccgattgttg cccaggtcac
     8881 ctttgccgat cgcgtgatga cgctggcctc tcgcagccag caggcaacga ttccggggcc
     8941 ggagtggcac ctgaatgacg aactggtggt gcgtagtctc ggttatcaaa cggtagaact
     9001 gaacgagttt attctgcccg ctaaagccgc caacgctgtc gagcgggtca aagacattca
     9061 gatccacaaa cagttgaatg gaccactttc gcagtttggg caacgcttct gggacgtgct
     9121 gtttaacgac catgaagaag cgcagtcact gatgaaaaac acgcgaatta ccggcgttca
     9181 ctataccgat cgctatctgc aaaacccggt tgcgctggcg ttgttgggat caattctgaa
     9241 accgctaaaa acaaagctga ccgatggcgc tgaagtggcg ctggatacgc tgtttaaaga
     9301 taaagatcgc cccggtaacc ggcctttcca tgactggatg agcatagcgg attttcagga
     9361 tttcgctgat cagtggtttg ctgctgcgct gggcaggccg atagaactga cggtgttcga
     9421 ttcgccgcgc gatatccctc accatcgtaa actgacggtg acttttgagg atggtcaggt
     9481 attgaagatc cgcttcgatc aggggatggg ctactggcgc atcaactttg catcgcaatg
     9541 gcattacttt gatttccgcg atgacgtttc tttccagtta gtcaaaatgg ctcaggcctg
     9601 caaggaaggg aatgtggcca acagcgaaga gagctgggca acggatgtgc tggtggaggt
     9661 gaccgcctcc tgatgatgtg ccgctcccga cgtcgtgtcg ggagcgtttt tcaattcagg
     9721 caactttctg tttacccgca attaccccaa taaaatcctg cacctgcttt tgtttacgcg
     9781 ccgacagctt gcacacctgg cgcagcgact gcatcagttc gctctcttcg gcgacgggtg
     9841 gttgggcggt gaggacaata cagccttcca ttactttgac atctaccgcc gtgccggtgg
     9901 caaaaccggc ggcttccagc cactgacctt tcagggtaat ggcgggaata cggctgtaat
     9961 ccgggtagcg gctcgcataa ctcacggtta attgacggtt atttgccggg gagacttctg
    10021 cttcgaacgg ttgtgcaata gaatgcgtgt cagtcatgat tactattctc cataaatagt
    10081 gattgtgatg agcggtgcgg gtgtgttggc gcacatccgc accgcgctaa atacctggat
    10141 atatcatcag taaatacagg aaaagtccag ctaaaaatag aataaaatgg gcaatttctg
    10201 gaatgaataa aatatatttg cgtgagttat gattggcgtg aaataataaa aagcgcaccg
    10261 gaaaggtgcg ccagaaaata atgatcagga ttttttacgc gaggcttttt tacccccgct
    10321 ggctgcgcgt tcagctttga ttttctccag caacgcggcg gcgctgtttt caccgctgat
    10381 taattccggg ttttcggccc gccactgggc agtgagctca ccacggaatg cttttgccag
    10441 gatggattgc gtcagattgt tgacgcgggc taaggcgttg ttgacctgtt tttctatggt
    10501 gtcggcgtag gcgaagagtt gctcgacgcg gcgaacgatt tcgcgttgtt cctccagcga
    10561 tgggagagga ataggtaact cttttaatat accaaga
//
LOCUS       DAZGCQ010000149        10449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000149 DAZGCQ010000000
VERSION     DAZGCQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.149"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..1848
                     /gene="ydeN"
                     /locus_tag="ACHK07_004516"
     CDS             166..1848
                     /gene="ydeN"
                     /locus_tag="ACHK07_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8104761.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHK07_004517"
     CDS             1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHK07_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8104762.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3023..3145)
                     /locus_tag="ACHK07_004518"
     CDS             complement(3023..3145)
                     /locus_tag="ACHK07_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104763.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            3347..5032
                     /gene="yddA"
                     /locus_tag="ACHK07_004519"
     CDS             3347..5032
                     /gene="yddA"
                     /locus_tag="ACHK07_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO8104764.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5070..7442
                     /gene="yddB"
                     /locus_tag="ACHK07_004520"
     CDS             5070..7442
                     /gene="yddB"
                     /locus_tag="ACHK07_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO8104765.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSNVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDLFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7499..10282
                     /gene="pqqL"
                     /locus_tag="ACHK07_004521"
     CDS             7499..10282
                     /gene="pqqL"
                     /locus_tag="ACHK07_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8104766.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLMALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3079 a         2306 c         2282 g         2782 t
ORIGIN      
        1 aaattactta gtagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt
       61 ttagctaaat ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt
      121 cgtaaaatta ctttggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta
      181 aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt
      241 gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca
      301 gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt actgaccatg
      361 gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg
      421 gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgcg
      481 caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc
      541 tatgtggcgc acggtgtttc cggcccctcc cgcgccgcaa taatgaccgg tcgagcgccc
      601 gcccgctttg gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact
      661 ttcttgcctg aattattcca aaatcatggt tattacactg cagcagtagg taaatggcac
      721 ttgtcaaaaa tcagtaatgt gccggtaccg gaagacaaac aaacacgtga ctatcatgac
      781 aacttcacca cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt
      841 atgggattcc acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt
      901 gaacgcgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt
      961 gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg
     1021 cacctgccaa atgataatcc tgcaccggag caatatcaga agcaatttaa taccggtagt
     1081 caaacggcag ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt
     1141 ctcgaacaac tgaagaaaaa cggacagtat gacaatacta ttattctctt tacctccgat
     1201 aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt
     1261 cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa
     1321 cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca
     1381 gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct gccctggttg
     1441 caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac
     1501 tggtttgatg aggaaaatat tccattctgg gataactacc acaaatttgt ccgtcatcag
     1561 tcagacgatt acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg
     1621 agaaataacg attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa
     1681 ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag
     1741 atgcaaggcg tggtaagaga gtttatcgac agcagccagc caccgcttag cgaagtaaat
     1801 caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat
     1861 gcggcggaat tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc
     1921 tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag
     1981 tttactcatg aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc
     2041 gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc
     2101 ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt
     2161 tttaatgcat tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa
     2221 gaacatgaat ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac
     2281 agacgcagta attcaggtaa cggtactttt acaaaagtga tagcagccat cgagcgtctg
     2341 aaatcatatc aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac
     2401 cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa
     2461 ttgctagaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt
     2521 atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg
     2581 caatgggtta aaaacgatgt gggtgaagtt ttcatccgtc agtttgaaag ctttgtcagc
     2641 cgttttttgg ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg
     2701 gttgttgaaa gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa
     2761 attggaaaca ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa
     2821 aaaaaacgga tttcagcgaa atgtctgaaa tgtgcatata aacctatctg caatggcggt
     2881 tgtcctaagc atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt
     2941 tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtaga gttagctaag
     3001 aacagagtac cgctttatca cattatggat gttgcaaaac aaatggagaa taattaataa
     3061 cacgccctct tagctccagt cttagcctac gtcttatgac cttaacaaag cacccgacat
     3121 aaattcctgt cgggtgtgtc gtcatcatca tcttaccttg cgaaaatcct ggcatacgtg
     3181 gtatgcgtca gggcattagc aatacccttg attatcaata gcataaccat aaccactgaa
     3241 attaaaaatt tctatatatt tcatattgtt aaatgatact taaataaatt tcatcagtaa
     3301 atcattgcca tagataatca ttggatggca caatcaattt tcgctaatga taaccattcc
     3361 cattacgttt tgtatgttga tagcaaagta tctctgtctg ctgaagccct tttggctgcg
     3421 taagaataat aaaacttcag tgttgttaat cattattatc ctcgccatga tccttggcgt
     3481 ggttaaaatt caggtctggt taaacgactg gaataatgat tttttcaatg cgctaagcca
     3541 gaaagaaacc gataagcttt ggcagcttgt cctttggttc cctgccctgc tggggatctt
     3601 tgtattaata tccgtaaaca aaacatggtt aattaaactg ctgactattc gctggcgcga
     3661 atggttaaca gattactacc ttaatcggtg gtttgcagat aaaaactact acttcacgca
     3721 gatctacggc gagcataaaa atacagataa ccctgaccaa cgtatcgctg aagacattct
     3781 cttactgatc agcaaaacgc tcagtctttc atttggcttc atccagtcac ttagcatgct
     3841 gatcaccttt accgttattc tttggcaaag cgcgggtacg ctctcattca ctgttggtgg
     3901 aacagaatgg aatatccagg ggtatatggt ctataccgtc gtgctcattg tgatcggtgg
     3961 aactttattt acccataagg tgggtaaacg tattcgtcca cttaatgtgg aaaaacaacg
     4021 tagcgaagcg acatttcgaa ctaatcttgt gcagcataat aaacaggcag aactgattgc
     4081 gctaagcaat gctgagtctc tacaacgcca ggagttgagc gacaattttc ataccattaa
     4141 agagaactgg catcgtttaa tgaatcgtca gcggtggctt gattactggc agaatattta
     4201 ttcgcgttcg cttagcgttc ttccgtactt tctgttattg ccgcagttta ttagcggaca
     4261 gatcaatctg ggcggactga tgaaatcgcg ccaggcattt atgctggtat cgaacaattt
     4321 aagctggttt atttataaat atgacgaact tgctgaactg gctgcggtta tcgatcgctt
     4381 gtatgagttc catcaactca ccgaacagcg ccctacgaat aagcctaaaa attgccaaca
     4441 tgcggtacaa gtggctgacg cgagtattcg tacccctgat aataagatca tattagagaa
     4501 cctgaacttt catgttccgc caggcaaatg gctattactg aaaggttact ctggtgcggg
     4561 aaaaaccaca ctgcttaaaa cattatccca ctgctggccg tggtttaaag gtgatatttc
     4621 ttctcctgct gacagttggt atgtgtcaca aacaccgtta atcaaaaccg gcttactgaa
     4681 agagattatt tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga gcgaagtact
     4741 gcatcaggtt ggtcttggga aattggctgc gcgtattcat gaccacgatc gctggggaga
     4801 tattctttcc agcggcgaaa aacaacgtat cgccctggca cgattaattt tacgacgtcc
     4861 gaaatggata tttcttgacg aaactacctc tcatcttgag gaacaagagg ctatccgctt
     4921 actgcgttta gtgcgtgaaa aactacccac aagcggcgtc attatggtta cacatcaacc
     4981 cggtgtctgg aacctggccg atgatatttg tgacattagc gcggttttat aaaaacaata
     5041 aaagccctgc tgtacggaga tataaataaa tgaagcgagt tcttattcct ggcgtcattt
     5101 tatgtggcgc tgatgtggcg caggccgtcg atgataaaaa catgtacatg catttttttg
     5161 aagagatgac ggtctatgct cctgtccctg tacccgtaaa cggcaacacg cattacacca
     5221 gtgaaagcat cgagcgttta ccgaccggga atggcaatat cagcgatctg ctgagaacca
     5281 accctgcggt acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga gatattcgcc
     5341 cggagaaaat ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg attgatggta
     5401 ttagtgccac taataacctg aacccagcga atgagtccga tgccagtagt gcaaccaata
     5461 ttagcgggat gtcacagggg tattatcttg atgtcagctt actggacaat gtgacgcttt
     5521 atgacagttt tgtgccggtt gaattcggtc gcttcaatgg cggggtaatt gatgcaaaga
     5581 tcaaacgctt caacgctgat gatagcaacg tgaaactggg ttatcgcact acgcgttcgg
     5641 actggttaac atcgcatatc gatgagaata acaagagcgc atttaatcaa ggttcttcag
     5701 gaagtactta ttactctcca gattttaaaa agaactttta taccttgtcg tttaatcagg
     5761 aactcgctga taactttggc gttaccgccg gtttatcgcg ccgccagtct gatatcaccc
     5821 gcgcggatta tgtttcaaat gacggcattg tcgccggtcg ggcacagtat aaaaacgtta
     5881 tcgatactgc attgagcaaa tttacctggt ttgccagcga cctctttacc cacgatttaa
     5941 ccttaaaata taccggctcc agccgtgatt ataataccag caccttcccg cagtctgatc
     6001 gcgaaatggg taataaatcc tatggtctgg catgggatat ggatacgcag ctcgcatggg
     6061 ccaaactacg taccaccgtt ggttgggatc atattagtga ttatacccgt cacgatcatg
     6121 acacctggta caccgaactt tcatgtacat atggtgatat tacagggcgt tgcacccgtg
     6181 gcggattagg acacatttcc caggctgtag ataattacac cttcaaaaca cgcctggact
     6241 ggcaaaaatt cgccgtgggt aatgtttcgc atcaacccta cttcggcgcg gaatacatct
     6301 attccgatgc atggactgaa cgccataacc agtctgaatc ctatgtgatt aatgctgccg
     6361 gaaagaaaac taaccatacc atttaccata aaggtaaagg cagcctggga attgacaact
     6421 acacactgta tatggcggat cgcattagct ggcgtaatgt gtcattaatg cccggcgtgc
     6481 ggtatgacta tgacaactat ctgtcaaacc acaatatctc cccgcgcttt atgacggaat
     6541 gggatatttt tgctgatcaa acctcaatga ttaccgcagg ttataaccgt tactatggtg
     6601 ggaatattct tgatatggga ttacgtgata tccgcaatag ctggacggaa tcggtatcag
     6661 gcaataaaac cctgacgcgt tatcaggatt tgaaaacgcc ttataacgat gaactggcaa
     6721 tgggattgca gcagaaaatc ggtaagaacg ttattgcgcg tgcaaactat gtttaccgtg
     6781 aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag cgcgactaaa accactatta
     6841 ctgaatataa caacgatggc aaaaccaaaa cgcattcgtt caacctcagt tttgagctgg
     6901 ccgaacccct gcatatccgc caggtagata ttaacccaca aattgtcttt agctatatca
     6961 agagcaaggg caacttgtcg ttaaacaatg gttatgagga gagcaatacc ggtgataacc
     7021 aggtggttta taacggtaat cttgtctctt acgatagcgt tccagtggca gattttaata
     7081 acccattaaa gatctcctta aacatggatt tcacgcatca accgagcggg ttggtgtggg
     7141 cgaatacgct ggcctggcaa gaagcgcgta aagctcgcat tatcctgggt aagacgaatg
     7201 cgcaatacat cagcgaatat tcagattaca agcagtatgt tgacgaaaaa ctggatagca
     7261 gcctgacctg ggacacccgc ttgtcctgga cgccacaatt tctgaaacaa caaaacctga
     7321 cgatcagtgc cgatattctc aatgtactgg atagcaaaac cgcggttgat acaacgaata
     7381 ccggtgtggc gacctacgcc agtggccgta ctttctggct tgatgtcagc atgaaatttt
     7441 aatagttcgg attttactta accgggtaaa ttcgcccggt tttcgcatgg agataattat
     7501 gagaaacctc tgtttcttac tgacgttagt ggcaactctg ttgctccccg ggcggctgat
     7561 tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg caattggaca atggcttgcg
     7621 atatatgatt tatccgcatg ctcatccaaa ggatcaggta aatttatggc tgcaaattca
     7681 taccggttca ttgcaggaag aagacaatga gcgcggcgtg gctcattttg tagaacatat
     7741 gatgtttaac ggcacaaaaa catggccggg taataaagtc atcgaaacat ttgagtcaat
     7801 gggcctgcgt tttggtcgcg atgttaatgc ctataccagc tatgacgaaa cggtgtatca
     7861 ggtgagtttg ccaactacgc agaaacaaaa tctgcaacaa gtgatggcaa tcttcagtga
     7921 atggagtaat gccgcaacct ttgaaaaact cgaagtagac gctgaacgcg gcgtaattac
     7981 tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc gccgcccttt
     8041 cctgctggct aatacccgta atttagaccg tgaacctatc ggcctgatgg atactgtcgc
     8101 cacggtcaca ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac caaataatat
     8161 gacctttatc gtggttggcg atatcgacag taaagaagcg ctggcgctga taaaggataa
     8221 tttaagtaag cttccggcta acaaagcagc tgaaaatcgc gtctggccga caaaagccga
     8281 aaaccacctg cgctttaata tcattaatga taaagaaaac cgggtgaacg gcatcgcact
     8341 ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa agctttatcg aacaagctga
     8401 atggagcatg ttagttcagc tgttcaatca acgtctgcag gaacgtatac agtcgggcga
     8461 gttgaagact atttctggcg gcactgcgcg cagcgttaaa attgcacccg attatcagtc
     8521 gctgtttttc cgtgtaaatg cacgagacga taatatgcag gatgctgcga atgcattaat
     8581 ggcagagttg gcaaccattg atcagcatgg tttttctgct gaagaactcg atgatgtcaa
     8641 atctacccgc ctcacctggc tgaaaaatgc ggttgatcag caagctgaac gtgatttacg
     8701 tatgctgacc agtcgcctgg catccagctc attaaataat acgccgttct tgtcgccgga
     8761 agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa gtctggcgga
     8821 aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg taaacaatga
     8881 ggttgccgca aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa aagagtacgc
     8941 caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa cagtagacgc
     9001 tgacccacag gcggaaatta gcagcaaaga aacgctggct gagaatctga catcattaac
     9061 actttccaat ggtgccaggg ttattctggc aaaatccgcg ggtgaagagc aaaagctaca
     9121 aattattgcc gtatcgaata aaggcgattt aagtttccct gcgcagcaaa aatcacttat
     9181 cgcgctggca aataaagcag ttagcggaag cggcgttggc gaactctcct cttccagcct
     9241 gaaacgctgg agtgcggaaa actcggtaac catgagcagt aaagtcagtg gcatgaatac
     9301 gttgctctcc gttagcgcgc ggactaataa ccctgaacct ggtttccagt tgattaacca
     9361 gcgaatcacc cacagcacga ttaacgacaa tatttgggca tcgctacaaa atgctcaaat
     9421 tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc agatgtatga
     9481 gacgcgctat gctgatgacc gcacgaaatt actgcaagaa aatcagattg cacagtttac
     9541 tgccgcagat gcgctggctg ccgatcgcca gttgttttca tctccagcgg atatcacgtt
     9601 tgtcattgtc ggtaatgtcg cagaagacaa actcatggcg ttaattacgc gttacttagg
     9661 ctcaatcaaa cactctgatt cgccattagc cgcaggtaaa ccattaactc gcgcgacgga
     9721 caacgcatcg gttactgtaa aggaacaaaa tgaacccgtg gcacaggttt cacagtggaa
     9781 gcgttatgat tcccggacac ctgttaatct ggcgacgcgt atggcgctcg atgcttttaa
     9841 cgtcgcactg gcaaaagatc tacgcgttaa tattcgtgaa caggcatctg gggcatacag
     9901 cgtttcttct cgcctctcgg ttgatcctca ggccaaagat atcagtcatt tgctggcttt
     9961 tacttgtcaa ccagaacgac atgatgaact gttaacgtta gcgaatgaag tgatggttaa
    10021 gcgtctggct aaagggatca gtgagcaaga actgaacgaa taccagcaaa acgttcagcg
    10081 cagcctcgat atccaacagc gtagcgttca acaattagcg aacactattg taaatagtct
    10141 tattcaatat gacgatcctg cagcatggac tgagcaggag caattgttga aacaaatgac
    10201 ggtagagaat gttaacactg ccgttaaaca atatctttct catccggtaa atacttatac
    10261 cggagtatta ttgcctaaat aataacagcc ccgtcaacac atcgttggcg gggattttag
    10321 caatattcgc tatttttatg taataatttt ataaatgcgt tcaaaataat aaccaagtac
    10381 taatagtgat attttaaggt ctgattttta cgtgataatt caggaggcac agaatgcgca
    10441 taaaaataa
//
LOCUS       DAZGCQ010000150        10210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000150 DAZGCQ010000000
VERSION     DAZGCQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.150"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(234..1634)
                     /gene="asnS"
                     /locus_tag="ACHK07_004522"
     CDS             complement(234..1634)
                     /gene="asnS"
                     /locus_tag="ACHK07_004522"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO8104767.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1802..3004)
                     /gene="pncB"
                     /locus_tag="ACHK07_004523"
     CDS             complement(1802..3004)
                     /gene="pncB"
                     /locus_tag="ACHK07_004523"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO8104768.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLRNFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3270..5882
                     /gene="pepN"
                     /locus_tag="ACHK07_004524"
     CDS             3270..5882
                     /gene="pepN"
                     /locus_tag="ACHK07_004524"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO8104769.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5925..6692)
                     /gene="ssuB"
                     /locus_tag="ACHK07_004525"
     CDS             complement(5925..6692)
                     /gene="ssuB"
                     /locus_tag="ACHK07_004525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8104770.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6689..7480)
                     /gene="ssuC"
                     /locus_tag="ACHK07_004526"
     CDS             complement(6689..7480)
                     /gene="ssuC"
                     /locus_tag="ACHK07_004526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO8104771.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7492..8637)
                     /gene="ssuD"
                     /locus_tag="ACHK07_004527"
     CDS             complement(7492..8637)
                     /gene="ssuD"
                     /locus_tag="ACHK07_004527"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO8104772.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQDLAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8634..9593)
                     /gene="ssuA"
                     /locus_tag="ACHK07_004528"
     CDS             complement(8634..9593)
                     /gene="ssuA"
                     /locus_tag="ACHK07_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO8104773.1"
                     /translation="MRKIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLATRPY
                     AVKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9586..10161)
                     /gene="ssuE"
                     /locus_tag="ACHK07_004529"
     CDS             complement(9586..10161)
                     /gene="ssuE"
                     /locus_tag="ACHK07_004529"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO8104774.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT         2537 a         2773 c         2627 g         2273 t
ORIGIN      
        1 cagacatcca gaacgccaaa aatagtatga aaattgtgct atcagctaag aaaaaactaa
       61 tccgcaataa gaatgcggat tattttttgc gcataaatgt tgcaatgtaa tattccgtaa
      121 caggatgatc gttatggcgg ggttgcaaat agattgcttg acttaacaaa cagataactt
      181 gacagaaaag ataaaaaaag agccagcggg cgctggctct tgaagacgct aaattagaag
      241 ctggcgttac gcggagtacg tgggaacgga atcacatcac gcacgttttg cacaccagtt
      301 acgtaagcaa tcaggcgttc aaaaccaaga ccgaaacctg aatgcggaac agtaccgtag
      361 cgacgcagat cgcgatacca ccagtaatct tctttattca ggcccatttc cagcatacgc
      421 tcgtccagca cgtccagacg ttcttcacgc tgggagccac caatgatctc accgatgccc
      481 ggagccagaa cgtccatagc cgcaacggtt ttaccgtctt cgttaagacg catatagaac
      541 gctttaatat ctttcggata gtttttaacc actaccggtg ctttaaagtg ttcttccgcc
      601 agataacgtt catgctcaga agagagatca acgccccagt aaaccgggtt ttcaaacttc
      661 ctgccgcagt tttcgagaat ggtcactgcg tcggtgtaat ccacctgcgc aaaatcggct
      721 tcaatgaagc gttccagacg tgaaacggca tctttatcta cgcgttcagc gaagaatttc
      781 atgtcgtcag cgcgttcttc gagaaccgct ttgaagacat atttcagcat ggcttcagcc
      841 agtcccgcga tatcgttcag gttagcaaac gccacttccg gttccagcat ccagaattcc
      901 gccaggtgac ggctggtgtt ggagttttca gcacggaaag tcgggccgaa ggtataaatt
      961 ttggacaatg cgcaagcgta ggtttcgccg ttcaactggc cagatacggt caggaaagac
     1021 tctttaccaa agaagtcttt gtcgaaatcc actttgccct gatcgttacg cggcaggttt
     1081 tccagatcca gcgtagaaac gcggaacatt tcgccagcac cttcggtatc agatgcggta
     1141 atcagtggcg ttgaaaccca gaagaatccc tgctcgttaa agaagcgatg cagcgcctgc
     1201 gccagcgtat gacgaacgcg cgcgacggca ccaatcaggt ttgtgcgcgg acgcaggtga
     1261 gcgacttcac gcagatactc aatgctgtgg cgttttgccg ccatcgggta cgtgtctgga
     1321 tcttcaaccc aaccagcaac ttcaaccttg ctggcctgaa tttcaaattg ttgcccctgg
     1381 cccggcgacg ccacgacttt acccgtcaca atgaccgagc aaccggtggt cagacgcagg
     1441 acgtcttcat tgtaattggg cagagaatta ttgatgacag cctgtacagg atcaaagcag
     1501 gaaccgtcat aaacggcgag gaaggagatg ccagcttttg aatctcggcg ggtacgtacc
     1561 catccgcgta cggtgacttc gctgtcaacg gctacgcggc cctggagtac gtcggctaca
     1621 ggcacaacgc tcataatatt ctctctgtta atagtcggaa aaaataaaca cttgtccacc
     1681 cgtaaatggg gggataccta tgttacctgg catctgcaat cagacaagca gaattcgcaa
     1741 atgcagcgaa agatttcgga attaaaggta aaaaagggag ccgattagct ccctgatgat
     1801 attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc cgaacaaacg
     1861 ctttatcgtg gcagatagtt ttgccagggc tgtcagaaag tttcgccacc ggtttaccgt
     1921 tacactctac caacttaatc acaatattca ggggttttac ctgggggata tcgcaggtca
     1981 ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg tatagctcaa
     2041 ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt ggatcaattc
     2101 ccagcttttc ataatgtgca atggcttttt caccccattc aaccgggtcg ccagagtcat
     2161 gacgcaggcc ctgataccga ctagcgaact cgacaccgaa atcacgcagg aaagcatcca
     2221 tagtgatgca gtcggttaat gcaatgccaa gttggtcggg atactcttcc agccaggcag
     2281 caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgt gcctggaacc
     2341 attcgtgtgc ctgtgttccc atcggcgtga gggaaagccg acgcgccaga tcgtagttgc
     2401 tggtgcccac gaaccaagat tcctgttgca gacgcttaac gatggtttct tgtacttcgc
     2461 gagaaaaacg gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata tcaagaccgg
     2521 cggttaacgc cgagaagtcg actaatttgc tttccagcgt gtcgagggct tgcgcaacgt
     2581 cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc aaaggaactt
     2641 cccagaggat gacttcacgc cacgggccgc ttaaacgaat atccagcttg ccattatcgt
     2701 tggacacggt gacttgttcc gggttaaagc ggaaattacg cagccagttg agatagtcgg
     2761 ccttaaagaa aggcagcgca gagagccatt gatattcatc atcctgcagg cgcaggtgct
     2821 gcatcgcctg aacctgttca cgaatagcat cggcataaat acccagcaga tcgtcacctc
     2881 ggcaacgaaa ctccgccgcg acatgcacat cgtaatagtg atgaaacacg gcttgctgca
     2941 tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa gcgaattgtg
     3001 tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac atcttcagga
     3061 actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca tatgccacct
     3121 ttctggtcga tggcattacg cggtgtatgt tataaaaatg tagcaataaa ggcgtttgta
     3181 cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga ctgaacacca
     3241 gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc aaataccgtc
     3301 acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt gacctcgacg
     3361 cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca tcagatgctc
     3421 cccttcgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat gatgagccgt
     3481 ggaccgcctg gaaagaagaa gagggcgcac tggtcatcag taatttgccg gagcgtttta
     3541 cgcttaagat cattaatgaa ataagcccgg cggcgaatac ggcgctggaa gggctttatc
     3601 agtcaggcga tgcgctttgc acccagtgtg aagccgaagg tttccgccat attacgtatt
     3661 atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc gataaaatca
     3721 aatatccctt cctgctttcc aatggtaacc gcgttgcgca aggcgaactg gaaaacggac
     3781 gccattgggt acagtggcag gacccgttcc cgaaaccgtg ctacctgttt gcgctggtgg
     3841 caggcgactt tgatgtactg cgcgatacct ttaccacgcg ttctggtcgc gaagtagcac
     3901 tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc gtgggcgatg acctcgctga
     3961 aaaactccat gaaatgggat gaagaacgct ttggcctgga gtatgacctc gacatctata
     4021 tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga gaataagggg ctgaatatct
     4081 ttaactccaa atatgtgctg gcccgcaccg ataccgccac cgacaaagat tacctcgata
     4141 ttgaacgcgt tatcggccat gaatatttcc ataactggac cggtaaccga gtgacctgcc
     4201 gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt cttccgcgat caggaattca
     4261 gctctgacct cggttcccgc gcggtaaacc gtatcaataa tgtgcgcacc atgcgcggat
     4321 tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat ccgcccggat atggtcattg
     4381 agatgaacaa cttctacacc ctgaccgttt acgagaaggg cgcggaggtg attcgcatga
     4441 tccacaccct gcttggcgaa gaaaatttcc agaaagggat gcagctctat ttcgagcgtc
     4501 atgatggtag cgcagcgacc tgtgacgatt ttgtgcaggc gatggaagat gcgtcgaatg
     4561 tcgatctctc ccatttccgc cgttggtaca gccagtccgg tacgccggtt gtgaccgtca
     4621 aagacgacta caatccggaa accgagcagt acaccctgac catcagccag cgcacgccag
     4681 ccacgccgga tcaggcagaa aaacagccgc tgcatattcc gtttgccatc gaactgtatg
     4741 ataacgaagg caaagtgatc ccgttgcaga aaggcggtca tccggtgaat tccgtgctga
     4801 acgtcaccca ggcggaacag acctttgttt ttgataatgt ctacttccag ccggtgcctg
     4861 cgctgctgtg cgaattctct gcgccagtga aactggaata taagtggagc gatcagcaac
     4921 tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg ttgggatgcg gcgcaaagtt
     4981 tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag ccgctgtctc
     5041 tgccggtgca cgttgctgat gctttccgcg cagtgttgct cgatgaaaaa atcgatccgg
     5101 cgctggcggc agaaattctg acgctgcctt cggtaaatga aatggcggaa ctgttcgata
     5161 tcatcgaccc gattgccatt gccgaagtcc gtgaagcact cactcgtact ctggcgacag
     5221 aactggcaga tgagctgctg gctatttaca acgcgaatta ccagagcgag taccgcgttg
     5281 agcatgaaga tattgcgaaa cgcactctgc gtaatgcctg cctgcgtttc ctcgcttttg
     5341 gtgaaacgca tctggctgat gtactggtga gcaagcagta ccacgaagcg aacaatatga
     5401 ctgatgcgtt ggcggcgctt tctgcggcgg ttgctgcaca gctgccatgc cgtgacgcgc
     5461 tgatgcagga gtacgacgac aagtggcatc aggacggtct ggtgatggat aaatggttta
     5521 tcctgcaagc caccagcccg gcggcgaatg tgctggagac ggtgcgcggc ctgttgcagc
     5581 atcgctcatt taccatgagc aacccgaacc gtattcgttc gttgattggt gcgtttgcgg
     5641 gcagcaaccc ggcagcgttc catgccgaag atgggagcgg ttaccagttc ctggtggaaa
     5701 tgcttaccga cctcaacagc cgtaacccgc aggtggcttc gcgcctgatt gagccgctga
     5761 ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat gcgcgcggcg ctggaacagt
     5821 tgaaagggct ggaaaatctc tctggcgatc tgtacgagaa gatcaccaaa gcactggctt
     5881 aataaataac cgaatggcgg caatagcgcc gccattcggg gattttaccc ctgttttctc
     5941 aggcgagttt cagactcacc acgctgcatt acccgttgca acacttccgc ttccagctcc
     6001 gctaatcgca ccgaccctaa ccggcgcgga cgggggatat ccaccgtcaa atccagacca
     6061 atttttccct cttcaattaa caacacccgg tcagccattg ctaccgcttc gctcacatca
     6121 tgcgtcacca acagtacggt aaagccgtgc tcctgccaaa gtgacacaat caaatcctgc
     6181 atctcgagtc gcgttaaggc atccagcgcc ccgagtggtt catcaagcaa caataaaccc
     6241 ggtcgatgaa tcaacgctct tgccagcgcc actcgctgtt tctgcccgcc agaaagtgcg
     6301 gcaggccatt ccccggcgcg attctccagt cctaccgcag ccaacgcccg acgcgcggca
     6361 tcgcgccact ggcctttaag acctaaccca acgttatcaa tcaccgattt ccacggcagc
     6421 agacgcgcat cctgaaacat cattcgcgtg tcttcctgaa tttcagccag cggtgtggtg
     6481 cccgctaaca catcgccagc ggttggcgtt tccagacctg ccagcaggcg cagcagggta
     6541 cttttgccac caccgctgcg gcccaccacc gccacaaact gacctgccgg aatatgtaaa
     6601 tccagttggt tcaggacgat attttctgcg taatgtttgc ttactgcatt gagcaacaat
     6661 ggtgtgccct ggttcagacg agcagtattc ataccgtggc ctccttcaaa tgataagccg
     6721 ggttccagcg caaccagagg cgctctaaca gctgcgcgct gacgtcagcc agtttgccga
     6781 gcagggcgta aagaataatg gcgaccacca ccacgtccgt ttgcaaaaac tcccgcgcat
     6841 tcatcgccag ataaccaatg cctgaattgg cagaaatggt ttcggcaaca atcagcgtca
     6901 gccacatcag gcccaacgca aaacgcacgc cgaccataat tgagggcagg gcaccaggca
     6961 ggatcacatg gataaacagc ggtatgccgg ataatccata gctacgcgcc atctccacca
     7021 acccgcgatc gatattacgg atgccatgcc aggtgttgat ataaatgggg aatagcgtac
     7081 cgagcgccac cagaaagatt tttgcggatt catcaatgcc aaaccacaaa atcaccagcg
     7141 ggatcagcgc cagatgcggc acgttgcgca acatctgaat tgaggtatcc agcagccgtt
     7201 ctccccagcg agatagcccg ctaatcagtc ccaaaatcaa ccccagcgat ccgccaattg
     7261 aaaagccaat cagcgcccgc caggagctga tcgccagatg ctgccacagt tcgccgctgg
     7321 cggagagcgt ccagaacgcc gtcaccactc cttccggtga aggcaaaata cgcgtcgaca
     7381 gccagccaac cgaggaggcc agttgccaca ccgccacgat gcccaccggt aaaaaccagg
     7441 gggcaacgcg caataaccac ttcttcactg gcgttgccat catcgtgttc cttagctttg
     7501 cgcgacttta cgggggataa aatcattcgc caccgcttcg ccttgcggat tcagcggctg
     7561 cggctgggga atttccggaa tggcgacatc cagatgtggg aacagcaact cgccgacccg
     7621 atacgcttct tccagatgcg gatagcccga aagcacaaag ctgtcgatgc caagcgcggc
     7681 atattcgttg attcgcgcag cgaccgtagg accatcgccc accagcgccg tcccggcacc
     7741 gccgcgcact aagccaacgc ccgcccataa attggggctg atctccagat tgtcgcgctt
     7801 gccattatgc aacgccgcca ttcgctgttg ccccacggaa tccgtgcggg caaacgcggc
     7861 ctgtgctttg gcgatagtct catcatcaag atgcgaaatt aaccgttcgg cggcctgcca
     7921 cgcttcgtcg ttggtttcgc gaacaatcac atgcagacga ataccgaaac gaattttgcg
     7981 tccatgcgcg gccgctttcg cccgcacttg ttcgattttc tcttttacca gctctggcgg
     8041 ttcgccccag gtgagataaa gatcaacttg ttcggcggcc agatcctgag cgacgtctga
     8101 agatccgcca aagtaaagcg ggggataagg ctgttgaatc ggcggaaaga gcagtttggc
     8161 tccacgcaca tgaatatgtt tgccgttgaa atcgacggtt tcgcccagca ataaacgccg
     8221 ccagacctgg gtaaattccg ccgaggcttc gtagcgctcg ctatgatcaa ggaacacgcc
     8281 atcgcctgcc agctcttgtg gatcgctgcc tgtgaccagg ttaaataacg cacgtccgtt
     8341 agagagacgg tcaagcgtgg cggcctggcg ggcggcaacg gtaggagacg tgacactggg
     8401 acgcagagcg acaagaaact tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag
     8461 ccacgcatct tcgcaggagc gccccgttgg gatcagcaca ccggtatagc ccagacgatc
     8521 cgccgcttgc gcaatttgtt gcagataacc gtgatcaacc gggcgtgaac cttcttccgt
     8581 tcccagataa tgcccgtcac cgtgggtcgg taaaaaccag aacatattca gactcataat
     8641 tgttttcctt ccagttgagt gggctgccag atgcgctggc gaatatcgac tttttttggc
     8701 accagacggt tttcataaaa cagatctgcc gtttgctgtt gtaaggcagc aacttctgcg
     8761 ctcaccggct tgatagtggt agggggacga tgatctaaat aagaggcaat caccggcgcc
     8821 ggtaagccca tcgtttttgc cagtaaagcg atactttgct cgcgctgact gcgggttaac
     8881 gcatcggctt cgctaaaggt tgccagtacg ccctgaataa atgcgccgtt ttttactgca
     8941 tacggacggg ttgccagata aaacgatccg gtttgattga ggtcggtacc gtctttcagc
     9001 acccgcacgc cgccctgtaa taacgcggcg gagtagtagg gatcccagat agcccaggca
     9061 tcgacgttac cctgctggaa tgcggcgcgg gcatcagctg gcgtcagata agtgggctgg
     9121 atgtcagtaa acttaagtcc ggcctggcgt agtgcgcgca gtaaaagatt gtgtgaactg
     9181 gaacctttct ggaacgcaac tttgtggcct ttaagatcgg ctacggtttt gatcgggctg
     9241 ttttctgcca ccagaatcac ctcggctttt ggcttcggcg gctcgacgcc cacatatacc
     9301 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
     9361 atactgccaa cgtttaacgc ttctaacatt tgcggacccg cggggaactc cacccaggag
     9421 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccaa taccatacca
     9481 atactgcctt tctgatagcc tatacgtaac gcttcaggcg aggattctgc agcaaccaca
     9541 atcgtagaga cgctaagcaa tcccaccagc gccagtttaa tgatcttacg catgggcatt
     9601 acctcgcaga gaatgaagat caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
     9661 agtttctagc gcggtgtcaa gacgggtttg taaattcggc gtgaactggg gtttgtgatg
     9721 gtaatcaatt acttgtgagt catcggcaaa cacgccgtgc aggatctcct gggctttcag
     9781 cgcacttaaa actggcttaa gggcataatc gaccgccagc agatgggcca ccgtaccgcc
     9841 cgtcgccagc ggtagcacca ctttgccttg caacgcacgt tctggcagca ggtcgagcag
     9901 ggttttcagc gcaccggaat aagcggcttt atacacaggc gtggcgacaa tcagcccgtc
     9961 agcctgttgc aactgttcgg tgaaggtatg gagtgccgga ctatcgaaac gagcataaag
    10021 cagatcttcc gcggcgaagt tttgcagatt ccagtgataa acctcaacat ccaggccatt
    10081 tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc gaggactacc
    10141 cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttcttgt tttgttaaca
    10201 ttgataacaa
//
LOCUS       DAZGCQ010000151        10050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000151 DAZGCQ010000000
VERSION     DAZGCQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.151"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..958)
                     /locus_tag="ACHK07_004530"
     CDS             complement(<1..958)
                     /locus_tag="ACHK07_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104775.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNTRDIRNIKNFSSHQYSLGDMF
                     DELINIIDKEQVLSTEQRKVIFRRYEDLYVKLMHYSVFTDQTHQIIKQKYFNDIVPMI
                     LALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPAN
                     AHFKDIFDGVYEFIRNIRKNSTPGKIKLIATINTCKETCKHLLYLSNEDKEKIISDLD
                     KIQVACYYLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQLLYLTNPIDVMNIL
                     NKYIYYFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYP"
     gene            1155..1848
                     /locus_tag="ACHK07_004531"
                     /pseudo
     CDS             1155..1848
                     /locus_tag="ACHK07_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2071..2367
                     /locus_tag="ACHK07_004532"
     CDS             2071..2367
                     /locus_tag="ACHK07_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8104776.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            2675..2791
                     /locus_tag="ACHK07_004533"
     CDS             2675..2791
                     /locus_tag="ACHK07_004533"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104777.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            3010..4443
                     /locus_tag="ACHK07_004534"
     CDS             3010..4443
                     /locus_tag="ACHK07_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8104778.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            5421..5984
                     /locus_tag="ACHK07_004535"
     CDS             5421..5984
                     /locus_tag="ACHK07_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104779.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHGCKHSMCMEIYRLLSETQTMLAGYYWVMEYTQNKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGDGYFHLCRAKDKYPVCIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            6124..6449
                     /locus_tag="ACHK07_004536"
                     /pseudo
     CDS             6124..6449
                     /locus_tag="ACHK07_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            6513..7075
                     /locus_tag="ACHK07_004537"
                     /pseudo
     CDS             6513..7075
                     /locus_tag="ACHK07_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            7261..7668
                     /locus_tag="ACHK07_004538"
     CDS             7261..7668
                     /locus_tag="ACHK07_004538"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8104780.1"
                     /translation="MADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDT
                     TPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAA
                     NTYNAEPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            7715..7988
                     /locus_tag="ACHK07_004539"
                     /pseudo
     CDS             7715..7988
                     /locus_tag="ACHK07_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8056..8234
                     /locus_tag="ACHK07_004540"
                     /pseudo
     CDS             8056..8234
                     /locus_tag="ACHK07_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            8515..8980
                     /locus_tag="ACHK07_004541"
                     /pseudo
     CDS             8515..8980
                     /locus_tag="ACHK07_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9069..9692
                     /locus_tag="ACHK07_004542"
     CDS             9069..9692
                     /locus_tag="ACHK07_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8104781.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYWYHLSHRIRRMVGSENTSSPYQQIAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            9788..10021
                     /locus_tag="ACHK07_004543"
     CDS             9788..10021
                     /locus_tag="ACHK07_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104782.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
BASE COUNT         2822 a         2011 c         2295 g         2922 t
ORIGIN      
        1 caggataaag ataaatatca gaaatactat agtctcgtat tgcgtcaatg gcatcccacg
       61 ataatgcact gtcaattttc agtgtataaa atggtgagtt ttcattagga aagtaatata
      121 tgtatttgtt cagtatattc attacatcta ttgggttggt taaatataat aactggcatt
      181 catattctga aacttctctt tcgcttatca gcattttata taaagttgcc agggtgcttg
      241 ttaatgaagt tcgtctttcg aaagccagta atatagtgag ataataacat gcaacttgaa
      301 ttttatctaa gtcagaaatt attttttcct tgtcttcatt acttaaataa agcagatgtt
      361 tacaggtttc tttgcatgtg ttgatagttg cgataagttt tatttttcct ggtgtactgt
      421 ttttgcgaat attacgaatg aattcatata cgccatcaaa gatatctttg aaatgcgcat
      481 tcgctggtgt gtatccggat aaacataact taacgtaatt tcgcagatat gtccttgcag
      541 catgatatat atcatcctca ttatccggta tctgagtgag aaaagaatga atatgatagt
      601 aaaatgccat ctcattatcc gggcgatatg tgttcctgat gtcgagtgcg agaatcattg
      661 gtacaatgtc attaaaatat ttttgtttta ttatttgatg tgtttggtct gtaaaaacag
      721 aatagtgcat tagcttaaca tagagatctt catatctcct aaatataact tttcgttgtt
      781 ctgtactcaa tacttgttct ttatcaataa tgtttattaa ttcatcaaac atgtcgccca
      841 gtgaatattg atgtgaggaa aaatttttaa tatttcggat gtcacgggta ttctggtttt
      901 tagccagaac accaaaacat gaaaagacat catacattat agtgataaaa ttatacataa
      961 cattctcctt ttattgcact atattaaatt ttaacattat atcattttgc ttttctgtta
     1021 actggttttc atttttgaat caatattttc tgaacagggt aatagtacac gtattaacag
     1081 attatgtcag aaggccccgg tgatgccttc tgacgataga ttaatgtggt attctattgg
     1141 tatctggaga ccatatggca acagttacag tacattctcc ccgctgtaat tcctataaag
     1201 tgtaccatta tgatcacagc acctcagatc atgagcgttt ccagtgctaa tcctgcaaac
     1261 gtgtgtattt caactgcatt acatctacga aacccgaaaa ccaggcatta aagagcaaat
     1321 tgttgaaatg gttcataacg gagtcggggt tcgcgatagc tccagaacgc tgaaggtgga
     1381 tatcaacacc gtcattctca ctttataaaa cgctcaccac gcaaagtaac cacatctcca
     1441 gtcgccagtg ccgatgtgac gcttatctgc gaacttgatg aacaatggag ttttgtcgaa
     1501 aataaagctc gtcagcagtg gcactggtat gcgtataaca ccaaatctgg aggtgtgctg
     1561 gcttacactt ttggtcctcg cactgatgaa acatgtcgtg agttgccgga actcctgaag
     1621 ccattctcca caggcatgat aaccagagat aaccggagca gttataccag agaaatgcca
     1681 caggataagc atcttgtcgg caagatattt acccggcgca ttgagcgcaa caatctgaca
     1741 tttcgtactc acatcaaacg accagcccgg aaaacgattt gcttttcacg ctccctggaa
     1801 attcacgaaa agccattggt tcatttgtcg aaaacactgt actactgact ggagtcatta
     1861 ccagggcctc ttttgctgtt ttttaccaat gaatgataat gagaatcgac agcgataagg
     1921 agaaagtaaa ataacaataa tgctgttgtt ttctatcgta ttgattttta attatttatt
     1981 gtctttttgc tcagcccatt tttttacgca gacaaattta cccggggaat acaaactgta
     2041 acgacagttc accacactat gggggataaa atgccagatc aatccggata ctgctggact
     2101 ccttcacgca tcttacggat gcctgaactg agccaattgt taggtatatc tcgttcgaca
     2161 atttacgaaa aaatgaatcc gttatcgaaa tactacgatg caaccttccc caggccggta
     2221 cgtcttggta gtggttctgt gggctggcgt tcatcagcta ttgacgaatg gcttacttta
     2281 catactgtac ctgccagatc agcagtcaaa ggtgttaatg acgaagtgcg acatacgctt
     2341 tcttcagata tgaccatgag cagataagtt tttgtcatca cgtgatcacc tcaggaggaa
     2401 aaagaagcat aagaacaatt tagccagaag tttaattgat aaacagaatt tccagcattc
     2461 attgttgaaa gagcagctat tgtcgctatt gtagcggtaa ttgctgctat aaccgaaacg
     2521 tattacgaaa aaggaagcat ttatgtctta catcaaaccg gatatgccgt attcagtata
     2581 tgcgtgacca tcaatgatag ggaaggcaat aattaaagct caatacaata aacaagtacc
     2641 tctgactctg gttgcaatgt cagtattaac agcgatgtca attgcctgtc agaatcaggt
     2701 tgacgtatgc agccccggaa atttacgtgg ggccgttaat atttacacta tggttctgga
     2761 gaaagggaaa agaccggggg aaaggtatta gtgaaataat ttcaggaggt attaagaatg
     2821 agaaatatga atgatatttc gtggtaaata atacagtctg cactgaatta accttatcgg
     2881 tttattaaac atcagatcta caaatgctct gtatttctca gagcatcagg tctgaatagc
     2941 agctattttc gctattgtag cgggatttgc tgctatttta ctaacccatt actgaaaaga
     3001 gaggctttta tgtgcttact tgcaccagaa aatccttatc cgatatatgc actaccaccg
     3061 ctggtgagaa atgcaataat tgaaactcaa aaaaatacac aggcaccttt ggctatggtg
     3121 gcgacatccg cattaacagc gttctcaagt gcctgtcaga atcagattga cgtgtgcaga
     3181 cctggaaatt tacgtgggcc tgttaatctt tactctctga ttctggctga ttccggtgaa
     3241 aggaaaacga ccgtggataa agtgtttatg aaagcatttt atctcaggga tgaagccctg
     3301 gcggaagaat acgcgaaact ggttgagaat tacagtacag aaaaggaaat atgggagcaa
     3361 aagcaaaaag cactggagtc aaaattgcat aaagaaattc gcgccggtaa agattgtaag
     3421 gcaacagagt cagagcttaa aagacatctg aataaatgtc ctgttccgcc gcagatacgc
     3481 cggacaattt tcaatgagac aacgatagag ggaatgttaa aatattattc cgacagcaat
     3541 cgctcttttg ctcttgtatc cagtgaagga ggaataattt ttgatggccg ggcaatgagt
     3601 aaacttggaa tacttaactc tttgtgggat ggtggttctc ttttcatcga caggaaatca
     3661 tctcccggaa ttattttgaa ggatccaaga ctgacggtat cggtgatgat tcagcctgat
     3721 gtttaccaga aaggtttttg tacgcgaaaa aaagaacttg tgaaaacgtc aggacatcat
     3781 gcaaggtttt tgatgtgtca accaacatca acgcagggga caaggataat aaccggagat
     3841 aattattcat cacagtatca ggatttgttt gaacagagga tcaatgagct tattgatgaa
     3901 aatctggcga tgagtggtga acgacgttgc cttcactttt ctcctcaggc tgccagaatc
     3961 tggacggatt attacaacga tgtggaatcg aagctggggg gattggggcc tttaagacat
     4021 tgccgggaat atgctgctaa aaatgcagag tatatggcaa gactggcagg acttattcac
     4081 cattcgagcg gtgaagaggg ggacatttcc ccttacactg cagaaatggc gagagaatta
     4141 gcaatatggt acggtaatga gtatgtgcgg ttgtctaatc cgttaacttt tgacaaccct
     4201 gccctgaccg tacctgtgcg actcattcca gaggagcttg aacttttcaa ctggataaaa
     4261 agctattgca ttgagaaggg gatcccctgt atgaaaaaaa atgatatttt acagcgtggt
     4321 ccgaaccgtt tccggaaaaa ggataaaatc aactggttac tggatttact gtatgaacaa
     4381 aacagagttg taccggttat tgagggaaaa acgttgtgtg tcgcacctaa ctttgacctc
     4441 tgaacgactt aatcgttgag taacatatag tctcctctgc tacttgcgga ggagactaat
     4501 gcctttacca cgtcagcgtt gttgtattag aaaactctgg tacaggtcag tcagtaaata
     4561 taactggtgt aaccactatt tactattgga tagagtgttg ctcaggatat tgtcggcttt
     4621 agtctgaggt gaggtgggac cttcattgtt ttcgtgttca ataaataatt taggttaatt
     4681 agtattatta atgggagtta tgttatctgt tatgttatct tatctctctc tttctttcgt
     4741 aatatcactc ctctttatgc taagtattct gtatgtgcgt aatacatata tctccataca
     4801 ctacagtgtg gtggtgtatg gagtttagtt atgtgtaaat tatgttacta cactcatagc
     4861 gtaatattga gaaagttaat ccactgaatg tatgtctggt ttgtatttaa cactagttac
     4921 gtaccagaac actattagag tatattgtct ttagttcgta cgttatagct ccgttacagt
     4981 ttatgggtaa atataatctt tttgtatgaa ataatcttct tattgtgaga ttaaatatct
     5041 gtaggctctc tctgcagagt ctctgttttt acttaatggt acggcaatac taatagcgat
     5101 gttgcctgtg gctattcgat agctgacttt tgtgaagatc agattacttc ctgtaagtga
     5161 ttgaatggta ctttcttaaa cttaattcta tgaggtgtgt ttttaccatt gtaattcgta
     5221 ctggtcctca gtttaagaga gtctcactgt attctgaagt attactccca ttactgtaat
     5281 gaggtaatac gaaacagttt tatcaggttg tctgtgctgc tggttacctc gtttattaat
     5341 gccgcggtac ggcgtttctg tactctcagt atttatgtaa ttccactttc ctgtcagttt
     5401 tataaagtaa ggactataaa atgtataatg ctaatcccaa ttacgaaatg gatttcatga
     5461 ttctgaaaga cgtgaatgaa cacatggaag gtctgtttca acgtttttct aaactgttac
     5521 ctttcagaat tgattttgcc tacaggaaag acacgcccag ttttggtcat ggctgtaagc
     5581 acagtatgtg tatggaaata taccgcttac ttagtgaaac ccaaacgatg ctggcgggat
     5641 attactgggt aatggaatat acacaaaaca agggacttca tattcatttt atcggttacc
     5701 tggatgggca acgtcacaag aactcttatc agatatccag gcaactggga gatatctgga
     5761 ggcggatcac ggaaggggat ggttattttc atctgtgccg ggctaaagac aaataccctg
     5821 tgtgtatcga tcacgtgatt cactatagtg ataaatccgc ggtagacgac ttacgttacg
     5881 ccctcagcta tctggctaaa caggatcaga aagaacacgg tattattctg ggccgtagca
     5941 ggcttcccga aaaaagtaac cggggtcgac cacgacataa ctgaattacg ggggcttatg
     6001 tccgaactat cgtaatttat tgcgtggatt tgttgttaac gtgtggcttt gtcctcagcg
     6061 caggggacta tgacacttca tcttctgtgg tgctaccccg tggggcaggt acaggtaacg
     6121 catatagttc ataaatctga cagtgatgaa ttagctgcac taagggcaaa cataacttga
     6181 agctcatggg attgaatggc gacgtaaacc gcagagccct gtgcagtgcg ttttcgtatt
     6241 atccacggat gagaaagttg cgttatttcg tcggttgttt cgtgggcgtg atgatgtatg
     6301 ggcccttcga tgggaaagca aaaccagcgg aaagggtatt cgtctgcctg gctaacgaat
     6361 ggctgttggg aatatgcggt aagccccgga taaaatgtgg ggactgcgct caccgtcagt
     6421 tgattcctgt atctgatctc atcatctacc tgaggtagtg tacaatatgc cgatatcgaa
     6481 ctgatactta attaaaatat ctagagtgtg atatatctct gagaactatc aatacgaata
     6541 ttaaaaacac ttccagaata acagcaatac ggggggatct ccattaccct cctgtgattg
     6601 acaacgggga tatcattacc tgtttcccta acctgatccc cggagagata tctcgtttcc
     6661 gtaactcgct gaatgcgaaa attaatgcag atcaacaccg taaaaaacaa caaggcaaac
     6721 gcacctacgc aacgtcggtt tgtattatct gggcgaaaga atatacgcag tcctttaaat
     6781 gtcattatca cgtctgcctt tttttaacaa agacgcctgt taccacctgg gaaactatga
     6841 gcgctctgac acactgagag ggttaattac cggagcctgg tacagtgcct tagggttgga
     6901 actggaagat caccagggat tagttcattt ccctgctaat ggacgttaca tcctggatat
     6961 taacgatcct gatttcccag agcagtacca gcgcttattg gcacgtctgg attatctgac
     7021 gaagctcgac actaaagcat caggccagcg taactttggc tacagcggtt tttgacctat
     7081 tgtaactgta aggtgattta ttatgtatac agaatcaaac attaagcttt cctgtaacgc
     7141 cctctggcaa aagtgtgtcc ttgcaactct gtatcgggct gctgctacac atcctaaaat
     7201 taaattatta cagtttaaat aatacattgc cctggtatga ataaggcaat tccttctctg
     7261 atggctgatt ttactggtga actgcataga ctaatttctg aacatttgca gtgtaagcga
     7321 cgtaaagata aatctgttga gcccacccct ttatattatc tctggagtag ggaggctgtg
     7381 gctaacgata ccactcctta tcttatggtg ctgctactta ataacgatgt ctggtgtaag
     7441 agtcgtgagg tggtggttct cagaaatctg attgagcagg cgtggggaaa agtattgggt
     7501 gaagataact ccattacaca tctggttgtg tatcctgaag tgctgtcgtt ggtatttgat
     7561 gcagctaaca catacaacgc tgagcctggt gtactggaac agacagcccg ggcttacatt
     7621 gaatcgcatg gtgctgttga acgtcatctt gggtgtagtg acttttaacg tcaggtgacg
     7681 cattgtcact tctgacctgc tacctgtacg ccggaatata atggtcagag aggcatattt
     7741 cgacgcccct aatctggggc gtttgattca ggatgtctgg aacaatccgg gctttgtgtc
     7801 accgggcgca tcaaagtagt accacccggg atgtggcgct ggatcgtcat gtaccggact
     7861 gcgaagacat acttaaagag gtgatatggg cgttcagtga ttttgtccgt gataatcgtg
     7921 gtgtgtatga tccggaagcc cgttaccccg ccggtaatcc ctggtatccc gtaacaggcc
     7981 agttctgaaa gcacagctca gcagattccc cgcattcgca caatcttcat aaatctcaca
     8041 gaggacattc tgaccatgtt gactacaaca agccacgaca gcgtatttct gcgtgccgat
     8101 aattccctga ttgacatgaa ctatatcacc agtttcaccg gtatgaccga cagaggggtt
     8161 tataagctga tcagtgaaga ttcatcaagc cgggccgatc cagccgcagg tataaaagcg
     8221 aagtgtcaca gtagatacag taacgaatcg aagaatcacg gtggcagaag tatgaaacgt
     8281 gttgtgatac cggtacgctg gcaatgttca acgtgtcagc gttggtattg tggcgctgtt
     8341 ccctggtgct ggtccccccg tgaatcttcc tgcgtcccct ccggtcagct ggcactttgt
     8401 catcctgact gattcatcat tcctttcata ttgccagtca cagctgtttg catcgaaccg
     8461 tacttgtgtt gatgcgtctg caccgactta accaagttca acagagataa aatagtgcaa
     8521 ataaccgaag ccttactttc agagccgggg gaaatccggc gttttgttca gcaggcggtg
     8581 gatcactggc cgcatctgct ggcaatccac ttcacacttc attcgacaga aggcgtctgt
     8641 ggtcaacaga ttcagacatt ctgtagttcc tttcaccgac gggtgcacga acgtattacg
     8701 gagcgtgatc acacggtcag cccatcagcg ccggtggtat tacgctggtt gcgggaacaa
     8761 catgaaggag caccgatacg atgtctgttg ctgctcagcc agacgagtgt ttgccatccg
     8821 cgggccagtg tcacagttga tgaagcatgt tcgcaagtgg cggatttact gcaacagacc
     8881 tggccggtga taagcgctgg cgctgacaga gcaatacgtt gcgttaaaaa cggctgttca
     8941 gtctctgatg ccacggggta ttgcaacaat catccggtga tggacgggag aaatatcgac
     9001 tcttccgtta gtttttacag actgataaag ttttgccagc tgaatcttta tacggaatac
     9061 tcttcagtat gcatacacga attgattatc tggcggataa atactgcttt acagaactga
     9121 atgagtcttc ccgtcttcgc ctgcagtggc aggatgtcct ggaggagtgc cggcagacag
     9181 aagccggacc agaagaacga ctgcgcattg ccctgctgaa tgtggattac gtcaccagtt
     9241 ttgaactgcc ttttcgcttg ttgcttaccc gtacaccaca actgattgcc gcgctgcgtg
     9301 aagaatggga tatcagccag aaaaatgtgg tgttcaacga taaacgcttt ggctgcgtgt
     9361 acagcctgaa ggccagcctt tccggtgtgc cggatacata ctggtatcat ctgtctcatc
     9421 gtattcgccg gatggttggg agtgaaaata catcatcgcc ataccagcag attgcccggg
     9481 aagtgaaagc gccccgtgaa cggctgaagt atgcgctgga ggcggggtta ctggtgactg
     9541 cactggacgg cctgttctgg tctggtagtc agcgtattgc agctgatgtt ttgaggctga
     9601 gaaaggccgg aatgccggtg gtgacaacgt ccgtggaagt gagcgataac ctgacgggaa
     9661 caacccgcaa aataccggca taccatctct gatacgacca tcagggggaa tattgctctg
     9721 gcagcagcag tacgacattc tttatacttt atccccgtgc ctgaaaacat gtgctaagga
     9781 aacgggaatg gcaaggtccg attacgatat tatcaatctg tctccggaac atgaactgaa
     9841 tgagtggctg gcagagagag gttatgccgg acaggcggat aaccgtaacc gggtggcaga
     9901 aattgttacc cgcaaattgc aggacagctt ttatatcaac gtttcctggg atgcgctgaa
     9961 cacggcatac agtgaacatc ccgagtggtt ttctgggctt gtctccgggg atgagaatta
    10021 ataagtggat tatgctgcca tcaggcagtg
//
LOCUS       DAZGCQ010000152         9923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000152 DAZGCQ010000000
VERSION     DAZGCQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.152"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..595)
                     /locus_tag="ACHK07_004544"
     CDS             complement(<1..595)
                     /locus_tag="ACHK07_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFO8104783.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(1073..1675)
                     /gene="fimB"
                     /locus_tag="ACHK07_004545"
     CDS             complement(1073..1675)
                     /gene="fimB"
                     /locus_tag="ACHK07_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO8104784.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            3130..3846
                     /gene="nanC"
                     /locus_tag="ACHK07_004546"
     CDS             3130..3846
                     /gene="nanC"
                     /locus_tag="ACHK07_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO8104785.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            3866..4972
                     /gene="nanM"
                     /locus_tag="ACHK07_004547"
     CDS             3866..4972
                     /gene="nanM"
                     /locus_tag="ACHK07_004547"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO8104786.1"
                     /translation="MNKTITALAIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALASFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRDNYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            5037..6017
                     /locus_tag="ACHK07_004548"
     CDS             5037..6017
                     /locus_tag="ACHK07_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8104787.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDALHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALTKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(6025..6675)
                     /locus_tag="ACHK07_004549"
     CDS             complement(6025..6675)
                     /locus_tag="ACHK07_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8104788.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIDQGFHIHQDKAAAAPIADVLFPD
                     ELPSQGTYVEGAAIKVTVNSYERDKKAREKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYLLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            6812..7684
                     /locus_tag="ACHK07_004550"
     CDS             6812..7684
                     /locus_tag="ACHK07_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO8104789.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIVLPQVRKNYPY
                     EGFIEWHRKEVFRG"
     gene            7729..7908
                     /locus_tag="ACHK07_004551"
                     /pseudo
     CDS             7729..7908
                     /locus_tag="ACHK07_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(8307..>9923)
                     /locus_tag="ACHK07_004552"
     CDS             complement(8307..>9923)
                     /locus_tag="ACHK07_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104790.1"
                     /translation="NDPKKMKRIFPLFIVSENINIHAEPDIEYFRVIDRAFDEVANYT
                     GRIFSYLRTHEPLKLTSCVNKQTLLSMGGYLNEWNVFDSLSRVRDFFRLSSAVFTKLD
                     NNIYSLEVDSFCLYRDYEIARNRLMMRASHLYSEVHEFSNKHFHLNSWVKDHMPSYLN
                     SDGVFSSFHLSELENISPDDLHEEYGNISLFNWVHAYQCLVELSKEEMSKRFSSTKPI
                     PLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDLNDCPFIPCMDGL
                     CLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAGIKASPIDAHANY
                     QCDCVILLDDCLIFTELKSNGQPIYYGKYYQQVCNIVGDSSLIHDHNNKFMRSYFQQI
                     NRISEHYLNHLDVIIKEFELPSTWQPKGVYKLIVTTTMLGGKYHVDDTYVADKYALSS
                     FFQRIPGVIYQTNENGKMAKNIIDGFECCEGEITIDKFIDYLSSLPSINAVRKNINKL
                     TYSVRFNEKLVHHPYYDSWAFGPYIRKGND"
BASE COUNT         3039 a         2055 c         2005 g         2824 t
ORIGIN      
        1 aaacctcttc tctttttaat ttttcgttta tgagattatt tctttcccat aatccggcaa
       61 aacgagcagc attactggcg gtataacgta cagtatggcg aatatttcga tgcccgagat
      121 aatcctgaat taaacgagta tctgcaccac gctccgccag ttcataaccg caagcatgcc
      181 ttaacatatg aggatgagtc tgcgttacgg ttccagcttc aataccggca tcacgaataa
      241 tgcgataggc ctgctggcga gaaagccgac tcccgcggcg agaaataaat atggcgtcgg
      301 tccggtcagc gcctttccag ttagcacgtt ccagggtcca gcgttccaca gcttcacgct
      361 catcaaaacg taacgggtga acggtagaaa atccgttctt cagtcggcga atatttattc
      421 gaccttcatt aaggtcaaga tcctgataat gcagatcaag cagttcacta atacgcatcc
      481 catgccgata tgccaacaga ataagacaat aatctctggc tcccgttgcc ccgtaacaaa
      541 ccgcctgcat catggcctga acttctttac cggtaagata acgacgttta ctcacaatag
      601 tagtactcct aactgagata tatttgaatg aatacctata ggaaacctca atcggtcaaa
      661 attagcccaa tagataaaaa gataaccaca cacgaagtga tgtggctatc agtcaattac
      721 attaaatatc atacaaaaat aaaatatcac tgatgtgaca acatctattt tcattgattt
      781 gattcaatat aacaatagat tgcagtggat taacttccag ttgcttaaaa aataaagaaa
      841 aaagtgcgaa aacaggagaa tttaagtgat gtgtaaattt atgaatgatt ttgtttattt
      901 attttgaaga acataaacca ttgtattttg tatggatatg gttaacttaa tgtttattat
      961 gataattaag aattatactt acaataacat caattaataa atggttaatc atttttgata
     1021 tatcgtaaga ataatgtagt ttttaacacc atccctggta tctcaactat ctctataaaa
     1081 cagcgtgacg ctgtcgtcct ctggctctat cccagatgcc gtaaaaacgc cctgcattgc
     1141 tggcggtata ccagacagta tgacgaatat tgcgatgccc aagataatcc tggataagtc
     1201 gcgtatctat tcccatattc gccaaagcaa aaccacacga atggcgtaac atgtgcggat
     1261 gaatctccag tgacaacccg gcattaccac cggaagtcga gataatatgg taaaactgtt
     1321 gccgagaaag cggattcccc ttacgcgata aaaataccca ctcgctctca gcatgcgggt
     1381 acgaagtacg gatactcaac cagtttttta aagcctgaac ttctttgttc aatagcgggt
     1441 gcgttgttga aaagcctttt tttaatcgat ggatatatat acactttgcc ttaagatcaa
     1501 tatccgaaat cctcaatcga caaatttcac tcgcccggaa accatgaata aaacaaagca
     1561 aagtcagaca ataattacgt gctgcatgag gcccggtatt tgctgcttta aggagtgatt
     1621 cgatttcact atgggtcagg aagttccttt ttttgttatc agccttattc ttcatcgttt
     1681 ttcccttata attacagacg cgcactagct gtgctgggtt agcaataatc caacattttt
     1741 atcctgatta tgtttataaa agcgaacgtt tgcttaataa ctaacgtaag tggaccagtt
     1801 cttctgagtg aacttaaatg gattagcaac agttaattat aataacgttg ccatacaaac
     1861 gctatgctct tgcatgctat gtacctttat atatttatca atcggataca aatctaataa
     1921 taaccctcat cattaatgca actaatctta tctatatatc atgtgatatg ttctgtaaca
     1981 agtaaaatca acataaaaaa ttcagcaaaa tttaatattc gttcaactag ccgtctcaca
     2041 tatgacatta ttagtcaact ccatttgcca caaaatggct atttcatgga acgcagtaat
     2101 ttcttagtgg ctattatcat gctaattctt aggtccctag cgattattcc tggctggttc
     2161 gtcagtttag gccatgagat tgttacattc ctttttatca tggagatatg ggggaaagca
     2221 ggaaacgatt ttttcatcta cattcgttgg taaatatagc tttggtcact acgacttaaa
     2281 ccttcctcaa tcacagccat tcgccgggcg atacggcaaa caaactatct gaaacatcat
     2341 cctgaaaaca ccaacatcaa caagcctctc cagatcgact tcagaagtga ccagttacaa
     2401 gccacaaacc aggaaacata tttcatgatg agaattatgc tcagcctgat ggcgggtaaa
     2461 cacttatttt caccacgccc actgagttag cttgcaaagc ttcccttgtt tagactctaa
     2521 ataaattatt tataaaacaa atagttaaat gaagatcttt agtttttaaa gaccaccgca
     2581 agtgttcgtc tggcttcaca tggcatcttc ttcttagaaa aagatcgaca tattttgtga
     2641 cacgaattgc aaatctggtt ttgttgtatg gattgcgtga tttttgatct ggtataacag
     2701 gtataaaggt gcaccacaat agacaatgag acagggcatc ttgcaatcta tggcaaacat
     2761 cacttcagtt ctttctcatc gggtgatgaa aacgcacttc agtctgaaag gaatatgaaa
     2821 atgagatcaa cagacactct attttatgac tctgggtaaa atggattgag taagtgatat
     2881 agcttacgaa cattaaaatc aattaaacat cagaagagat tttatactca ggtatttaat
     2941 ctggctctct gtttatttaa ataacgtgga aagagatttt tcacaggaga cattatacaa
     3001 aaaatataaa atacagctac cggttgccaa agacactata agcctggcaa aaaatattac
     3061 acaacataaa tgctaattgt ttatgcgggc tttgtattgc tttctgtatc ctacaaatga
     3121 gtgaaattta tgaaaaaggc taaaatactt tctggcgtat tattactgtg cttttcgtca
     3181 ccattaattt ctcaggctgc gacacttgac gtacgtggtg gatatcgtag tggaagccac
     3241 gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa atggatggtg ggcaagcatg
     3301 gaaagtaata cctggaatac cattcatgat aataaaaagg aaaatgccgc actcaatgat
     3361 gttcaggttg aagttaatta cgcgattaaa cttgatgatc aatggacggt gcgcccggga
     3421 atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa actgtcctgg
     3481 gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa agcttaccga
     3541 caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga tggatatgtc
     3601 acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata cagcaaacag
     3661 aacgattatc gctatgcaaa ccataagaaa tgggcgacgg aaaatgcatt tgttctacaa
     3721 taccatatga cgcccgatat tacgccatac atagaatatg actacctgga ccgtcagggt
     3781 gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt gtcatttaaa
     3841 ctgtagtaga caggagacag tcacaatgaa taaaacaata acggcgcttg ctatcataat
     3901 ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat ttaaaagtgg
     3961 taccggagca attgataacg acactgtcta cattggttta ggtagcgcag gtacggcatg
     4021 gtacaagctg gagacacagg ccaaagataa aaaatggaca gcgttagctt cattccctgg
     4081 cggaccaaga gatcaagcaa cctcggcatt tattgatggc aatctgtatg tgtttggcgg
     4141 cattggcaaa aacagcgagg gcttgactca ggtatttaat gacgtacaca aatacaaccc
     4201 caaaaccaat agctgggtta aattgatgtc gcacgcaccg atgggcatgg cgggtcatgt
     4261 gacttttgta cacaacggca aggcttatgt tactggcggt gttaaccaga atatcttcaa
     4321 tggctatttt gaagatctca acgaggctgg aaaagattca accgctatag ataaaatcaa
     4381 cgctcactat tttgacaaaa aagcagaaga ttatttcttc aataagtttc tgttgtcttt
     4441 tgatccctca acccagcaat ggagttacgc tggcgaatcg ccctggtacg gaacggctgg
     4501 tgcggcggtt gtgaataaag gtgataaaac ctggcttatt aatggtgaag ccaaaccagg
     4561 attgcgaacg gatgccgtat ttgaacttga tttcaccggt aataatttaa aatggaatag
     4621 gcttgctccc gtctcatcac cagatggcgt cgctggcggt tttgcgggga taagcaatga
     4681 ttctcttata tttgccggag gggccggatt caaaggttca cgagataatt accagaacgg
     4741 taagaactat gcgcatgaag ggctgaaaaa atcatatagc gctgatattc atctttggca
     4801 taacgggaaa tgggataaat cgggtgaatt atcgcaaggt cgggcctacg gagtatcatt
     4861 gccctggaat aatagtctat tgattattgg cggtgaaact gcaggcggca aagcggtgac
     4921 ggattcagtt ttgatctctg tgaaggataa caaagttact gtgcaaaact aacgcttcag
     4981 ggccccagta aaggggccct gctatcaatt tgtcatttca attacgcgaa aattatatga
     5041 acgcaataat atcgccggat tattactatg ttcttaccgt tgctggtcag tctaatgcca
     5101 tggcgtatgg cgaaggactg ccattaccgg acggggaaga tgcgcttcat cccagaatta
     5161 aacaattagc gagatttgcg catacgcatc ccggaggccc gtcatgtcac tttaacgaca
     5221 ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat caccatcctc
     5281 tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat attgcacgga
     5341 aattactgcc cttcattcct gataatgcag gggttctcat cgttccatgt tgtcgtggcg
     5401 gctcggcttt tatcgcaggc agcgaaggga catattcaga acggcacgga gccagccatg
     5461 atgcttgtcg ttggggaacg gatactccgc tataccagga tttagtcagc agaacgcgag
     5521 ccgcactgac aaaaaatccg cagaacaaat tcctcggcgt atgctggatg caaggcgaat
     5581 ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat atggttgaag
     5641 cctttcgtag ggatctaaaa caataccatt ctcagcttaa taatattact gacgcgccgt
     5701 ggttttgcgg cgataccacc tggtactgga aagaaaattt ccctcatgcg tatgaagcta
     5761 tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac ttccagcaac
     5821 aaggtgaaag aggactgacg aacgcgcctg atgaagatcc ggacgattta agcacgggat
     5881 attacggttc agcgtaccgc tcaccggaga actggacgac ggcactgcga agcagtcatt
     5941 tcagcgcggc agcccgtcgg gggattattt ctgacaggtt tgtagaagca attttgcagt
     6001 tttggcgcga aaggtgagcg ttcattaatt tgatttattc gcatccatca acgcttttaa
     6061 ctcctccggc gtaaacggtg gtttgcgtcg gtgtaacatc gcatggcaat tagggcacac
     6121 aggaagtaaa tcattaactg gatccagcag gtaatcctct ttaatggctg aaagcggcac
     6181 caggtgatgc atatggataa agccctcagc gatatcgcca tagatcttca ccagatcaac
     6241 gccgcatacg ttgcactggc atccgtgata ttcgactgct ttctcgcgag cttttttatc
     6301 gcgttcatag ctgtttaccg tcacctttat cgccgcacct tcaacgtagg tcccctgcga
     6361 cggcaactca tccgggaaca gaacatcggc tatgggagcc gcagcggctt tatcctgatg
     6421 aatatgaaat ccctgatcta taagtagctt gacgcattta gattttattc cgccggtgaa
     6481 atccgcaggg gtaaattgcg tccccgtcat caatgtcgct gcgatgccag caatcgcctt
     6541 cgaggggtaa cgtcggccat gatattcaat ctcatacaac cgtgcaggct ggaactgatg
     6601 agtttctccc gcatcccata aatgaatagc ttcgataata taccgccgcg gaagatgatc
     6661 ggggagttta ttcatagaga atgtccttat acgttctcag aacaagcagc aaaaaaacaa
     6721 atgagatcaa agtaatatat cttcacttgc aggcttgaac agcagaaaat ttgcgagcat
     6781 gatcgcaatt atttcactgc cagggatatc aatggcttcc agtaaatcgc tacagcaggc
     6841 aattgccaac ataaaaatct ggcacaaggg tgaacagcgc gcgccgcata aaccattgtt
     6901 actgctatac gtattagcgg gatacctgaa tggacatcca cgccttttcg attatggatc
     6961 ggaaatctac gaacctctgc acagtttact ggaacgtttt gggccacagc gttcacagta
     7021 ccgacctgac atgccgttct ggcgattaca aggcgacgga ttctggcaat tacacaatgc
     7081 cgagctttgc tcaacagcag ggagcagccg acaaccgcca gtgaaggaac tgaacgaata
     7141 ccacgttgca ggcggtttcg atgaacagca ctacgccctg gtaaccggca ataaaaaact
     7201 catcaatact ctggcgcaac agatactcga agcgcacttc accgagagta ttcaggaaga
     7261 aatcgctgac gagctgggtt ttgatctcca gcaaatccgt aaacagcgcg acccgttatt
     7321 tcgcaaaaat gtattacgcg cttataacta ccagtgtgcg atatgtggct ttaatatgcg
     7381 ccacgacgac accaccgtcg cgctggaagc ggcgcatata aaatggaaac agcacggcgg
     7441 tccttgtgaa attcctaatg gcctggcgct ttgcgcaata caccataaag cctttgataa
     7501 aggatcgatt ggcctggacg aaaatatgcg ggtactggta tcagatgcgg taaacggcgg
     7561 agggatcgtt gagagattat tctgggattt tgacgggaaa actattgtgt tgccccaggt
     7621 gcgtaaaaat tacccttatg aggggtttat agagtggcat cggaaagagg tttttagggg
     7681 gtgaggttga taactatcgc agtatgttca atattgctga cataatcagt gcaggatctt
     7741 gccgctagtt tacaattcgc cttcgatagc ggcgcaaaac gagttctgtt gccgatatcc
     7801 tcggctatgg atattccaac agttccggca gagttgttta ccaagtttca ggtaagtttt
     7861 tactcagaac cggttgatgc ggtttataag gcgctggatg tgaattaaca cattcctcag
     7921 cttatttata aaaggattat tcccatttga tataatatca gtgccaattt acttttttca
     7981 atggaatata tctcggctaa ataacaataa aacttatcgc taatggatta aataaacaat
     8041 tttctaattc atttcaccac ctttgatata gaaattgatg gtgttgtcgc tgccattgct
     8101 tatggtgatg ataaaatcca cgtttctgga tcgttgcgta gaaaatcatc actaccttga
     8161 tattttgatg cgatatgatc ataccgttcc gtagtaccaa tttcaatttc aggctaaacc
     8221 ccaacattaa aaaaattaga gttattcact gattatatta ttaataatat aatcagtgaa
     8281 taacatggac tatataataa attgctctaa tcatttccct tacgaatata tgggccaaaa
     8341 gcccaagaat cataataagg gtgatggact agtttttcat taaatcgaac actataagtt
     8401 aatttattta tatttttacg tacagcatta atactgggca aagaggaaag ataatctata
     8461 aacttatcga tagtgatttc cccttcgcaa cactcaaacc catcaatgat atttttcgcc
     8521 atttttccat tttcatttgt ttgatatatc actccaggta ttcgttgaaa aaaactagat
     8581 aatgcatatt tgtcagctac atatgtatca tccacatgat atttcccacc gagcatagtt
     8641 gtagtaacaa taagcttata cacacctttg ggttgccatg ttgatggtag ttcgaattct
     8701 ttaatgatca catcaagatg attaagataa tgttcagaaa ttcgatttat ttgttgaaaa
     8761 taagaacgca taaatttatt attatgatca tgtattaatg agctatctcc aacgatgtta
     8821 cacacttgct gatagtactt accataatat attggttgac catttgattt tagttctgta
     8881 aaaatcaaac aatcatccag gagaatcacg caatcgcact gataattagc atgagcatca
     8941 attggagaag ctttaatccc cgcatcacga acttgtttta taaattgttg ctcatgaaat
     9001 cgccctttac ttgcttgaga aatttttttg gaaccaaata atgacattag agagcgagtc
     9061 actgagctat ttgctattaa tgcaggcatt aagcataaac cgtccataca gggaataaat
     9121 gggcaatcat taagatcatg tgattttgaa ttgaaggtaa aataatctat cagttttttt
     9181 gctgcatctg ctgcaatccc cttacgttga aagaatgata accaactctc acggcttttt
     9241 atgatcagcc agcgatccaa ctgtaggggt ataggttttg tcgatgaaaa tcttttgctc
     9301 atttcttctt tagataactc gactaaacat tgataagcgt gcacccaatt aaataatgat
     9361 atattcccat attcttcatg aaggtcatca ggagatatat tttccaactc gcttagatga
     9421 aatgagctaa aaacgccatc agaatttaag taagatggca tatgatcctt tacccaacta
     9481 tttagatgaa aatgtttgtt actaaattca tgaacttcgc tatatagatg tgacgctctc
     9541 atcattagtc tatttcttgc aatctcataa tctcggtata aacaaaaact atcaacctca
     9601 agagagtaaa tgttattatc caattttgta aatactgcac tacttagtct gaaaaagtct
     9661 cttactctgc ttaatgagtc aaaaacattc cactcattaa ggtaccctcc cattgaaagc
     9721 aaggtttgtt tattcacaca agaagttagt ttcaatggct catgagtccg taaataagaa
     9781 aatattcgtc cggtgtaatt ggctacctca tcaaatgccc tatcgataac tcgaaaatat
     9841 tcgatatcag gttcagcatg aatattaata ttttcagaaa cgataaataa tggaaaaatc
     9901 ctcttcattt ttttaggatc att
//
LOCUS       DAZGCQ010000153         9817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000153 DAZGCQ010000000
VERSION     DAZGCQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.153"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..117)
                     /locus_tag="ACHK07_004553"
     CDS             complement(<1..117)
                     /locus_tag="ACHK07_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104791.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKISKMMNGKLIYSWL"
     gene            195..1289
                     /gene="ddlA"
                     /locus_tag="ACHK07_004554"
     CDS             195..1289
                     /gene="ddlA"
                     /locus_tag="ACHK07_004554"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8104792.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1313..1525)
                     /gene="yaiZ"
                     /locus_tag="ACHK07_004555"
     CDS             complement(1313..1525)
                     /gene="yaiZ"
                     /locus_tag="ACHK07_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO8104793.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1785..2093
                     /gene="yaiY"
                     /locus_tag="ACHK07_004556"
     CDS             1785..2093
                     /gene="yaiY"
                     /locus_tag="ACHK07_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO8104794.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2152..3246)
                     /gene="yaiW"
                     /locus_tag="ACHK07_004557"
     CDS             complement(2152..3246)
                     /gene="yaiW"
                     /locus_tag="ACHK07_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO8104795.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSRAPQPLKKGEKEIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3259..4479)
                     /gene="sbmA"
                     /locus_tag="ACHK07_004558"
     CDS             complement(3259..4479)
                     /gene="sbmA"
                     /locus_tag="ACHK07_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO8104796.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4831..5988
                     /gene="ampH"
                     /locus_tag="ACHK07_004559"
     CDS             4831..5988
                     /gene="ampH"
                     /locus_tag="ACHK07_004559"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO8104797.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5989..6612)
                     /gene="iprA"
                     /locus_tag="ACHK07_004560"
     CDS             complement(5989..6612)
                     /gene="iprA"
                     /locus_tag="ACHK07_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO8104798.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6700..9597)
                     /locus_tag="ACHK07_004561"
     CDS             complement(6700..9597)
                     /locus_tag="ACHK07_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8104799.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYVDTNKPIYN
                     IDTDYLTYSGYVYNTVAKDSGYYDTVFSGDTVNGVISTYYLNYDDYAAGTANTVSINN
                     STVHGMITSKYVPNFGDAAKAHDWIDGDTFTLNISNSTIDDDYEGFYFTDTYLDADGK
                     TVKSDNYEYSVAGMGTAVTLDVESNINISNNSHVAGITLEQNDTTNTAYKTGATQWDN
                     TITVNDSTVTSGSWTEDEQQDGHFGNSAEPSDYSGDGRVGYHSNDNALEFIDDSSSDY
                     RMVNNVTFNNSQLMGDVYFQSTWNNNFDYIGHPTDTDSNVYTHGGWIDDDQNVDHLTL
                     TLNHTKWVGGAYIDYAGDDIVTPDHFYDVAANSLDPNATNYDGWNRMDSAASFQSGVF
                     DVVLNDGSEWDVAKDSRIDTLAVNNSQVDVANGVELTSDTIGLNDGSVLNIGEGGQVN
                     TDHLTVNTFSTVTLANDISSDWALDALYANTITVTNGGVLDVQTSNTDAANVFTTDTL
                     ELTSGNVKDNHGNVYAGVFDINSNDYILNADLVNDRTNDTSKANYGYGVIAMNSDGHL
                     TVNGNGDINNVDTAATNQGGEADNSTADNVVAATGNYKVRIDNSTGAGAIADYAGKQL
                     IYIDDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNI
                     WNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKI
                     DGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFN
                     NDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQL
                     SNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSI
                     DNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2534 a         2431 c         2333 g         2519 t
ORIGIN      
        1 aagccaggaa taaattagct tcccgttcat catttttgag atcttacgca catctttgag
       61 ataacgcact ttcttcacca gtacgttgca cgctttgtca gcagaagatt gcaacaatct
      121 ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcacttta tgttagacgg
      181 gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt aaatcagcgg
      241 aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat aaaagtcgct
      301 tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc gatgccagca
      361 attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc
      421 tcgcccaggt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac ggtcagccgt
      481 tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa gatggttcct
      541 tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt ctggcttcag
      601 cagcctgtat ggataaagat gtcaccaaac gtctgctacg cgatgccggg ctgaacattg
      661 cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc gaagtggagt
      721 ctaaactggg gttaccactg tttgtaaaac cggctaatca gggctcttct gttggtgtca
      781 gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga tctggcgttc gagttcgatc
      841 ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat cgaatgcgca gttctgggca
      901 acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat ttctatgcct
      961 acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca gccattgcgc
     1021 cagaaatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg
     1081 caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga
     1141 tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg caagccagcg
     1201 gtctgggtta caccgatctg atcacacgtt tgattgagct ggcgctggag cgtcacgctg
     1261 cggataacgc attgaaaacc acaatgtaat attttaacgc ccgataatat tcttattggg
     1321 cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc cgccagccag aaactaatca
     1381 cccacgtcac cagaccgacg gcataggtct gccaaccttt tgcttcaaat cccagtaacc
     1441 ccaccacgcc attaagaatg aatataaggc caatggcgaa cgcatagtag tgccagtcac
     1501 ggcggatttt tacaggcaga ttcatggctg ctccgttaac gaaaaacaac atccttgcat
     1561 attctgcaac aaccgtctgt ggtgtagaca acattttcgc gatagttaat aaatttaact
     1621 taaagttgct attttgtgac tcgcaacgaa gattcacgct tcgggaatat tcccaacatc
     1681 aatttaccat gattctgata ttgacaatct aaatgacctg tcagacttgc cataagccgc
     1741 tggtatcaca ccagaagaga agcagatgac ttccggaggt ttttatggct gatttcaccc
     1801 tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct ggcaacattg
     1861 cctatgcgtt gtttgtgctg ttttgctttt gggctggggc gcaattgctg aacctgttag
     1921 tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg
     1981 tggaaattgg tttaggtgtc ggcaccattt tcgggctgat cccgttttta gtaggctgcc
     2041 tcatttttgc agtagtggcg ctatggctgc actggcgaca tcgccgccag taagatttac
     2101 gttgccagat acggcctaaa acgccttatc tggcatgaca ttcctgtttg atcactgttt
     2161 catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag tcaggttgcg
     2221 ggtgattttc gggctttcca gttgaatgcc aggcaacatt tcgcggggga gtgatttacc
     2281 cgttttcgct tcggcaagtt gataaacttt cttgtacagc gccgtctcct caaaagagaa
     2341 gctgtcacct ttctctagct gacgacgaat ttcgctgtcg ctcatcccca gttttgcagc
     2401 cagtttgcgc gttgccagtt cggttttccc gggttcttta ctgtcgtaac gaattaaatc
     2461 accgtccagt gccagcttca cgccgctggc cttactgacc gcgttctgaa acgcggcatt
     2521 acggctggcg taccagccag cgttaaaatc agcaaagcgg tatatcggtg cgctatagct
     2581 ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc gactgaagac
     2641 ttcctgacgg actgtaccgt ccattttcca cggataccct ttggtatgct gttcggcaaa
     2701 agcaatgctg acctgcatcg gcccaccggt gcgcaccgga ttaagcgaac caaacagcgt
     2761 ctgccccatt ggcaccatgc tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt
     2821 acgcaccgaa tctaaccgtt cgctataact tttgccgttt ggcgatttga tttttagcgc
     2881 ggtatgaacc agaaaaggag gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca
     2941 ggcgattttg cttaagcccg gaacggccgg atctgcctga taatttgact cctgctgcgc
     3001 caccgccagc accgagcaga cgttttccag cgtcggtgcc agcccctgac tttcaaaggt
     3061 ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg
     3121 cacgacgctc gccacatcta tctctttttc gccctttttc aacggttgcg gagcccgact
     3181 actacacccc gctaaaacca gaaccgccaa caatgacagc gaagatagag gattgacgcg
     3241 cgacattact tctccttttt agctcaaggt atgggttact tcctgaattt tgtcaccatc
     3301 cagctcatgt tcaaagctgc gcagacgttt gtagatagac atcaactcaa ccagtgtggt
     3361 ccatgagtta atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt tggtaatctg
     3421 cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca agaacaaacc
     3481 gaaaacgtta tcgacctgca aatagagaat gcgagcgatg ttgaaataca tatagtgaaa
     3541 atagaggcgg aaatagtttt tccgtacggc gctaaacagc tcgcgtaccg ttggcggcgt
     3601 cgcgcgcgtg gcatcgtctt caccataaac cagctcttta cggtaggcag cctctactcg
     3661 ctggttttta aactccagcc ccggcagttt gatccctacc actgccagca atccggtccc
     3721 catcagcgac cagacgatag cggcaatcac cagaccatac ggaatgtgcc cgataatcgg
     3781 cagctccggc acatgcgcgg agagcgttac cagcaccggc aggaaggcga tcaacgtcat
     3841 gatggcgttg ataaaactga cacccatatt ctccagcgtt gaagcaaaac gcatggtgtc
     3901 ttcctgcaca cgctgtgcgg cgccttcgat atgacgcagt tgttgccagt tcgccatgta
     3961 atattcgttc atcgccgtac gccagcggaa cacgtagtga ctgacaaaga agttattcag
     4021 cacactgatc actaccgcga tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa
     4081 ttgttcgata gtgactttat gcggcgaact tagcgcggtt tgaatcagat catagaacgg
     4141 tgcataccag gcgttgacgg caaccccgac ttccaccaaa aaccaggtga cgaagatgat
     4201 cagtgcagta ccgagtattg accagtattg ccaacggtgc gggctgtaga taaaccagaa
     4261 caatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga aatccaacga
     4321 ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg ccggtgatac gcgccaccca
     4381 gtcaccccca ccggcttgcc agaagataac ggcaatcaat gcccaaacaa aggccgagag
     4441 aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata tgcactcctg ctaacttctt
     4501 atcgttttaa caactgctgt gtattaaccg catgaccgct acaaagcgtc agccaaatga
     4561 aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc agcctggcca taagaattgt
     4621 ttcgacggat ttcacaaacc aaagattgtt tacataagcg gattaacacg cgcaggatca
     4681 caaatgcagg cattggtgag caggaaaaag atcgtgttta cgcgccgtta cgtttttaac
     4741 actattattg cactaattcg ccctttgcaa tccatcaatg agtagtataa atacgctcag
     4801 ttaccttcat tcaatttatg gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg
     4861 ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc cgatcactga accggagttt
     4921 gcctctgata ttgtcgatcg ttatgccgat catatttttt acggcagcgg tgccacgggg
     4981 atggcgctgg ttgttatcga cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga
     5041 cctggtaata acgttcgccc gcagctggat tcggtcgtgc gtattgcttc cctcaccaag
     5101 ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg ggaccgtgaa gttaaacgat
     5161 ccgctaagta aatatgctcc gccaggcgca cgcgtgccaa cctacaatgg gacgcccatt
     5221 acgctggtca atctggcaac ccataccagc gccctgcccc gtgaacagcc cggtggcgcg
     5281 gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac gctggaaata cctttctacg
     5341 gcgaagctga aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc gttcgacctg
     5401 ctggcagatg cactggcgaa tgcgtccggc aagccttata cccagttgtt tgaagagcag
     5461 attacccgtc cattggggat gaaagacacc acctacaccc cctcaccgga tcagtgccgc
     5521 cgtttaatgg ttgcagagcg cggtgccagt ccatgcaata acacgctggc ggcaattggc
     5581 agcggcggcg tctattccac gcctggcgat atgatgcgct ggatgcagca gtatttgtca
     5641 tctgattttt atcagcgcag taaccaggca gatcgcatgc agacgttgat ttatcagcgc
     5701 gcgcagttta cgaaagtgat tggcatggat gtccccggca aagcggatgc gcttggttta
     5761 ggctgggtat atatggcccc gaaagagggt cgtccgggga ttattcagaa gacaggcggt
     5821 ggtggcggtt tcattaccta tatggcgatg atcccgcaga agaatattgg cgcgtttgtg
     5881 gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat caatgatctg
     5941 gtgaccgagc taagcgggaa taaaccgttg gttatccccg catcctgatt aatactctga
     6001 aggcaaacgg ttgatagcga ccagtttgcc tttattcatt tcgatatagc cgcctttacg
     6061 caaagcagcg agaacttctg cgacgacaga acgcgatatg cgtgtacgtt gatggatata
     6121 attcatcaca ccaatacgtg atcgcaattc ttcattccag tcaatcattg ataataatgt
     6181 tgcgcggatt tgttcgtagg aattatgccc aatgagctgc acgtcgcgta attccagaat
     6241 tctattttgc caggctaacc agtaaaaagc gtctcgccag agttgatttt gttcaataag
     6301 cgtaatggtt tgtttggctg gtagatgata tcccgtacaa tttccttctg atattaattt
     6361 gtatggtata tcgtttttca ttaaaccatc agccagcccc ataatataag gagcctgggt
     6421 aataccgata agtacgtttt cttctctacg cagagagata acgccctcta aaataacgaa
     6481 agtatcttcg ttattgacat cactggaaaa tataacttgc ttttcattat taaactcgaa
     6541 gcgcgtaccg tatctggaca aacatttatc gagcttacca aattcctgaa gaggtttaac
     6601 tacagataac atttgcgcgt cctttgcagt aatgcccgtc aaatccttga cgggcattat
     6661 ttagattaaa ttaccagaat ttcttcggag tgaagaatat taccaggtat atttaacacc
     6721 cacgttcgcg gaccaatctt gatctacgtc accaccaccg aggtagttag catcggtata
     6781 ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta ccgcagaccc
     6841 ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta tcgttgttag agtcgtcgta
     6901 gacgtaagcc agtttgaagt acggagtcag agcctggtct tcgctgtaag tgaaggtata
     6961 acctgcatct acacccagtt cataacgcat gctgtcgtaa gactgaccgt caactttcat
     7021 gtcgttgctc agctggtagt catcaccaga ctggaacaga ccagaaatgc tgccgtaagg
     7081 agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca
     7141 agcgtcggag ttggtgctac cgtcaacgta agtaccgttg ctcatggttg cagacaggtc
     7201 gttgttgaag tgagagtagc tcaagctacc atcaacaaag acgttgttcg cgaagtgagc
     7261 agaagagtag atgtaggcag tctggctgtc ttgatccacc tgaccagaac ggtcattcat
     7321 gtcaccttta gcgaagcctg cagccgcacc gacgatccac ttagcgttgt taccgtcaat
     7381 tttggtatca acaccgacca tgatgccgtt aacatcctga tcatagttga tggtgccgtt
     7441 gtcaccgttg aagttaccac cgaagtagct tacccatgcg ccgccgttat cagccaggcc
     7501 atgacgagag ttggtcaaac gagtaccaac ggtgtcttgt tccaggttcc agatattggt
     7561 gttcgcagac gggatgctca gcgccatgtt agcgtagtca gtcagctcca tctgttgcag
     7621 aacaacggtg ttaccgcgct gttcagcctg ataggtgtat gcacccaggt cagctttgtt
     7681 agcagcagag aaggtcgcgt tggttttggt atcatcgata tagatgagct gcttacctgc
     7741 gtaatcagca atcgcaccag cgccagtgga gttgtcgata cgaactttgt agttaccggt
     7801 tgcagctaca acgttgtctg cagtgctgtt atcggcttca ccaccctggt tggttgcagc
     7861 ggtatcaacg ttgttgatat caccattacc attaacagtc aggtgaccgt cagagttcat
     7921 tgcgattaca ccgtaaccgt agttagcttt gctggtgtcg ttggtacgat cgttgaccag
     7981 atccgcattc aagatgtagt cgttgctgtt gatatcgaac acaccagcat aaacattacc
     8041 atggttgtct ttcacgttac cgctggtcag ttccagagta tcggtggtga agacgtttgc
     8101 agcgtcagta ttactggtct gcacatccaa tacaccgccg ttagttacgg tgatggtgtt
     8161 ggcgtacagt gcgtcgagtg cccaatcgct gctaatatca ttagctaagg tgacggtact
     8221 gaaggtgtta acagtcaagt gatctgtatt aacctgacca ccttcaccga tgttcaaaac
     8281 agaaccgtca ttcaggccga tagtgtcaga cgtcagctca acaccgttag caacatcaac
     8341 ctgagagttg tttacagcca gggtatcaat gcgagagtct ttcgccacat cccattctga
     8401 accatcgttc agaacaacat caaacacgcc gctctggaaa gaagctgcgc tgtccatacg
     8461 gttccaacca tcatagttag tagcgtttgg atccaagctg ttagccgcaa cgtcatagaa
     8521 atgatccgga gttacgatgt catcaccagc ataatcaata tacgcaccac cgacccattt
     8581 agtatggttc agggtcaggg tcagatgatc gacgttctga tcatcatcaa tccatccacc
     8641 gtgggtatag acgttggaat ctgtatcagt tggatgaccg atgtaatcaa agttattatt
     8701 ccaggtactc tggaaataaa catcacccat caactgagag ttgttgaagg taacattgtt
     8761 gaccatgcgg taatcagaag aactgtcatc aatgaactct aaagcgttat cattgctgtg
     8821 gtaaccgaca cggccatcac cagagtaatc actcggctca gcagaattac caaagtgacc
     8881 atcctgctgc tcgtcttcgg tccaagaacc agaagttaca gttgagtcat taacagtgat
     8941 ggtgttgtcc cactgagttg cgccagtttt gtacgcagtg ttagtggtat cgttctgttc
     9001 cagcgtaata cctgcaacat gagagttgtt gctgatatta atgttgcttt caacatccag
     9061 cgttactgcg gtacccatgc cagcaactga atattcatag ttgtctgact taacagtttt
     9121 accatcagca tccaaatagg tgtcggtgaa gtaaaaacct tcataatcat catcaatcgt
     9181 tgaattgctg atgttcagag tgaaggtgtc accgtcaatc cagtcgtgag cttttgcagc
     9241 gtcgccgaag ttaggaacat attttgacgt gatcataccg tgaacagttg agttattaat
     9301 actcacagta tttgcagtac ctgctgcata gtcatcatag ttcaggtaat aggtagaaat
     9361 tacaccattt acagtgtcac ctgagaaaac ggtgtcatag taaccgctat ctttagctac
     9421 tgtgttataa acataaccac tgtaggtcag gtaatcggta tcaatgttgt aaattggttt
     9481 gttggtatca acataaccaa aagtattata agtagcagca ttagcctgag aggtgatagc
     9541 cagagtgcaa gccaatgcta attgtgatac tacaagtttc tttttccagg agtgcatttg
     9601 tcatccctcc tcagggacgt aataagttga tccatcaatt atcaatgcat agaaaacatc
     9661 ttatgtttcg cgtttacgat tatgcggcgt tatagaggaa tggttcaatt attctttccc
     9721 ctaagtccga ttgcggacat atataaaaat atctatacgc acaaagaata agttgacaaa
     9781 aacactgtat ttcaacacgt taatgaaatc attaatt
//
LOCUS       DAZGCQ010000154         9817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000154 DAZGCQ010000000
VERSION     DAZGCQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.154"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            331..945
                     /gene="evgA"
                     /locus_tag="ACHK07_004562"
     CDS             331..945
                     /gene="evgA"
                     /locus_tag="ACHK07_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO8104800.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            950..4543
                     /gene="evgS"
                     /locus_tag="ACHK07_004563"
     CDS             950..4543
                     /gene="evgS"
                     /locus_tag="ACHK07_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO8104801.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAEQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADFYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVVHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(4599..5744)
                     /gene="yfdE"
                     /locus_tag="ACHK07_004564"
     CDS             complement(4599..5744)
                     /gene="yfdE"
                     /locus_tag="ACHK07_004564"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO8104802.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5818..6762)
                     /gene="yfdV"
                     /locus_tag="ACHK07_004565"
     CDS             complement(5818..6762)
                     /gene="yfdV"
                     /locus_tag="ACHK07_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO8104803.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6832..8526)
                     /gene="oxc"
                     /locus_tag="ACHK07_004566"
     CDS             complement(6832..8526)
                     /gene="oxc"
                     /locus_tag="ACHK07_004566"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO8104804.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8580..>9817)
                     /gene="frc"
                     /locus_tag="ACHK07_004567"
     CDS             complement(8580..>9817)
                     /gene="frc"
                     /locus_tag="ACHK07_004567"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO8104805.1"
                     /translation="QGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQL
                     RDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGF
                     TWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAA
                     ALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGY
                     LEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTC
                     KAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVL
                     SMKEISIDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSDFTPDIKAAPLLGEHTAAVL
                     QELGYSDDEIAAMKQNHAI"
BASE COUNT         2906 a         2235 c         2019 g         2657 t
ORIGIN      
        1 gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa aaacaacaga
       61 ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt gagaagactt
      121 atagtgctaa tgtgaaacta taaatcatcg gtacaatccc tgattttatt gttgacattt
      181 catatatgcc gactatttat atggtatact tgtcgaatta tcttaaagga agctcaaatt
      241 ttcttatttt tattgagaaa atgagatgat gccttatgtc tgtattacta cagggagaag
      301 ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga tgaccatcct
      361 cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat cttagcagag
      421 ttgactgaag gcggaagtgc cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc
      481 attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct gaggaagcgc
      541 caatatagcg gaattattat tatcgtctcc gctaaaaatg accattttta cgggaaacat
      601 tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa caatatcatt
      661 gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct caaccggttt
      721 gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt
      781 gtcatgcgct atattcttga tggcaaggat aataatgaca ttgctgaaaa aatgttcatc
      841 agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga atgtaaatca
      901 ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca tgaagttttt
      961 accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg cagacgaaga
     1021 ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc cactacgtct
     1081 gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg cagtacacaa
     1141 gtcccaaacg gctacgttgt tgcataccga ttcgcagcaa cgggttcgtg gtattaatgc
     1201 tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc
     1261 cgatcatcaa aaagcaatgg acgcgctgga agaaggtgaa gtcgatatag tgttatcaca
     1321 tttagttgct tcgccgcctc ttaatgatga cattgctgca accaaaccac tgataattac
     1381 ctttccggcg ctggtcacca cccttcacga ttcaatgcga ccgcttacct catcaaaacc
     1441 agtaaatatt gctcgagtag caaattatcc ccctgacgag gtaattcatc aatcatttcc
     1501 aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg tctcagccga
     1561 acagaatgat tactttattg gtagtaacat cattaccagc agtatgattt cccgctattt
     1621 cactcactcc ttaaatgtag tgaaatatta taactcaccg cgtcaatata attttttctt
     1681 gaccagaaaa gaatctgtcg ttcttaatga agtactcaat cgatttgttg atgctttgac
     1741 aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc tggcctttct
     1801 gaacaaacca ttagaactca ctgaacatga aaaacaatgg attaagcagc atcccgattt
     1861 aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa atggctcggt
     1921 tcggggcgtt atgggggaca ttcttaatat tattaccttg caaacaggtt taaatttttc
     1981 tccgatcacc gtttcacaca atatccatgc tggaacacag ctgaatcccg gcggatggga
     2041 tatattaccc ggtgctattt atagtgaaga tagagaaaat aatgttttat ttgctgaagc
     2101 cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg aacaaacatt
     2161 aaaaaaagga atgaaagttg ccattccata ttattatgag cttcattcgc aattaaaaga
     2221 gatgtatccg gaggtagagt ggataaaagt cgataacgcc agcgctgcat ttcacaaggt
     2281 caaggaaggc gaacttgatg ctctggtcgc gacacagtta aattcacgtt acatgatcga
     2341 ccattactat cctaatgaac tttatcattt tcttattccc ggcgttcaga atgcatcact
     2401 ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata aagcactgaa
     2461 tgcaatcccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt
     2521 gaccattgac acatgggacc tctatagcga gcaattttat attgttacga cattatccgt
     2581 tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag ttcgtcgtcg
     2641 taaagtcatc cagggtgatt tagaaaacca aatatcattc cggaaagcac tctcggattc
     2701 cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc ataatagtgc
     2761 ttttgaacat tatttcactg cggatttcta taaaaatgca atgttaccat tagaaaacag
     2821 tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag aaacgaaaga
     2881 aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga aaagatgcat
     2941 taatcactgg catacattat gcaatctgcc agccagcgat catgctgttt atatttgtgg
     3001 ttggcaagat attaccgaga cgcgtgattt aattcatgca ctcgaagtag agagaaataa
     3061 agcgatcaat gcaactgtcg caaaaagtca gtttctggca acaatgagtc acgaaataag
     3121 aacaccaata agctccatta tgggcttcct ggaacttctg tcgggttctg gtcttagcaa
     3181 ggagcaacgg gtggaggcga tttcactagc ctacgccacc ggacaatcac tcctcggctt
     3241 aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca
     3301 atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg cgattgctgc
     3361 aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc tggttaagat
     3421 cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc tcaaatttac
     3481 caccgagggg gcagtaaaaa ttacgacctc cctggttcac attgatgaca accacgctgt
     3541 aatcaaaatg acgattatgg attctggaag tggattatca caggaagaac aacaacaact
     3601 gtttaaacgt tatagccaaa caagtgcagg tcgtcagcaa acaggttctg gtttaggctt
     3661 aatgatctgc aaagaattaa ttaaaaacat gcagggtgat ttgtcattag aaagtcatcc
     3721 aggcatagga acaacattta cgatcacaat cccggtagaa attacccaac aagtggcggc
     3781 tgtcgaggca aaagcagaac aacctatcac actacctgaa aagttgagca tattaatcgc
     3841 ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat taggatatga
     3901 tgttgacgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc aacattatga
     3961 tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga ctcgcaaact
     4021 ccgtgagcaa aattcgtcct tacccatctg ggggcttaca gccaacgcac aggctaacga
     4081 acgtgaaaaa gggttaaatt gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga
     4141 tgtactgaaa acacatttaa gtcagttaca ccaggttgtg catattgcac ctcagtatcg
     4201 ccaccttgat atcgaagccc tgaaaaataa tacggcgaac gatctacaac tgatgcagga
     4261 gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt ttcatgcact
     4321 agaagctggc gataacagaa ctttccatca atgtattcat cgcatccacg gtgcggctaa
     4381 catactgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa cacctgtttc
     4441 agatgacagt aaacctgaaa ttcttcagtt gctgaactct gtaaaagaac acattgcaga
     4501 gctggaccag gagattgctg ttttctgtca acaaaatgac taaatagcgg ctcccacaat
     4561 gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa ctcctggcga
     4621 atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg gtccgcgcag
     4681 ccactgattt ttatcggatt acccggcatc attattcccc cggcttcaat caacatattt
     4741 ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg cgcgacgggc
     4801 acaccaactt catgtattct ggctaaccaa acttcagctg cctgtgtttt taacgtccgc
     4861 tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct gctaaatctg
     4921 ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagagaa aagcttgtca
     4981 ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa aggtgccatg
     5041 taggggtggc gatttcccaa acgttgtggt gacttcccag tcgcgatata tgccatcagc
     5101 ccgtgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg cgcccctctc
     5161 tggctctttt cgcggccata aagtgcactc actattccac tgaataaata gacaccgcca
     5221 catagatccg caagagaagt gccaacgcgc actggcggag catcagggta tcccgtttcc
     5281 atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc ttttagcgga
     5341 ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat ttcttgtaac
     5401 ctttcccatg aaaaccccag tttttccatt gtacctgggc gaaaattctc agctaataca
     5461 tcagcttgtt taagcatatt tataaatata cttttatcgt gatcattctt taaatcaaga
     5521 accacactct ctttaccatg attaataaaa ctgtaataga gtgactgtcc atccacatag
     5581 ggaccaaatg tgcgggtatc atcaccatga cctggtggct caactttaat tacccttgcg
     5641 cccatattac aaagaagttg agttccgaaa gggccattaa ggacatgtgt catatcgata
     5701 actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt atgccttgaa
     5761 aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca tgattaatta
     5821 tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaaac ccagtacgct
     5881 cacagccaat gacgctgtac cagtgcgggt gtagacatta aaccggctgg caataatgat
     5941 tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat gttcgctgtt
     6001 caaatgacat gccataccaa caagaagcag cgccagtggc atcagaatca gcttcaggaa
     6061 ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag tcaaccccgc
     6121 agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg ggtcccatgc
     6181 tgccggaatt tttaccccaa ccagcaccag gatcgttgcc agaacaggtg cccatactac
     6241 aggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac catccgctcc
     6301 tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa caataataga
     6361 aataattgct accactaaac ctgttgatac ggaatcacca taaataggat cgagaactgc
     6421 aaatcccagg aatccaatgg taggtgaacc tgcaattaat gcacatacag ctgcttctgc
     6481 atgggtacgt ttaaaaaatt tgtagcaacc aaaccaggag aaaaagaaac atccgacaat
     6541 aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc tgtttgcccg
     6601 ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt tattaaatgc
     6661 ccgcgcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca acagcatgat
     6721 tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat ttccgataag
     6781 ggtcccctca ccttcgcata atgagttggc aagaggagac gatgtcagta tttaattacc
     6841 agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg ctgcagggtc
     6901 gatgaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta aagcatgacg
     6961 aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca ttaatttgtc
     7021 ataccttgca tggtgcaaca gatctgttgg tgatggtgca ccagcgccac tgagatcaac
     7081 accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg gcaggttata
     7141 tcgacaaatc gtttcaatct ccatcccact gaaaccaaaa gcactatcac cttcaatggc
     7201 gacaaccgga gaaccagagg tcacgctagc accgatggca tagcccatac cgatgcccat
     7261 gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa taatatttcg
     7321 tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt tctcacgcaa
     7381 tacatcgcgc acagcactta atgcattaaa gtaatttaat ggttgggtat ctgtacttaa
     7441 tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat cgcgccatac
     7501 cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca tactggatgc
     7561 aatatcgcca acgactggca cagcaatggg gcggttgctg tcaatttcct gcggttcaat
     7621 atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg ccagtaacca
     7681 attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa acgaacgcgc
     7741 agctgccgca gaaagtggat gcgtatcttc gaggatccct ttcgccatag acattggcag
     7801 gaatggaatc tgggcacttt caataaattc acgaagctgt tcgtcagctt gtgaatacgc
     7861 cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg aaattgcgct
     7921 agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt ttacaatcgt
     7981 ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca aatcaagata
     8041 aactccgcca gggcgacccg atactgagac ccggatagcg cgtgccaatg caatgccaag
     8101 atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg cattcatttg
     8161 gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc tggagccgct
     8221 aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgcggtca aaccattgag
     8281 gaatcctggc gcagacactg tcaggcagat ccctggtttt tgggtaagaa aaccgcttgc
     8341 cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa tgccttccgc
     8401 ctgggcatgg cgcgccatat ccgtcacagg aatacctaca acaccataaa tagtgtcaat
     8461 attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt gaagttgatc
     8521 tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc ccgttaatat
     8581 cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc cagctcctgc
     8641 aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg cgtaaagtca
     8701 gagaatttca ttggacaacc aactgtcaga tattttccac gcaacggttg ttccacttcg
     8761 acaacactgc cactttggcg caaagaggga tcaattgaaa tttctttcat acttaaaacc
     8821 ggtgcacaag gaatatcaaa ttgagtcaaa taggccaccg cttcatgttt atcaatagtg
     8881 acagtgtatt tttcaatttc agcaaaaata tcgaaaatat gtggctgtcg ggcatgggct
     8941 gtactgtatg ccggatcggt aatccaatct ggtttgccga tggctttaca ggtgttttcc
     9001 cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc ccaaccttta
     9061 catttcagga tccagccagg ctgaccgcca ccacccgcat tacctccgcg gggaactgca
     9121 tcaccaaatg tgccattcgg atactgcggg tattcttcca gataacccaa tttatcgaga
     9181 cgctgctggt cgcgtaattt cacgcggcaa aggttcaata cggcatcctg cattgacatg
     9241 gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag taaaccgatc
     9301 agtaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag cggcggaccg
     9361 tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt ttcataggct
     9421 tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa aatcagacgt
     9481 ggattgattt cttgaatatg ctcccaggtg aaacccatgt gatcaatggc ccctggatga
     9541 aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc tttgccttcc
     9601 gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt gaagtaaagc
     9661 gcatcgatat caggaatatc tcgcagctgg tgacgcgtta cgtcaccaac gccgggacgt
     9721 tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga tgggccagat
     9781 tgcacaccgg tgaaatcgag aactttaatt ccttgaa
//
LOCUS       DAZGCQ010000155         9716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000155 DAZGCQ010000000
VERSION     DAZGCQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.155"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..107
                     /locus_tag="ACHK07_004568"
     CDS             <1..107
                     /locus_tag="ACHK07_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8104806.1"
                     /translation="ISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHK07_004569"
     CDS             complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHK07_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO8104807.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            948..1568
                     /gene="fklB"
                     /locus_tag="ACHK07_004570"
     CDS             948..1568
                     /gene="fklB"
                     /locus_tag="ACHK07_004570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8104808.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1877..3289
                     /gene="cycA"
                     /locus_tag="ACHK07_004571"
                     /gene_synonym="dagA"
     CDS             1877..3289
                     /gene="cycA"
                     /locus_tag="ACHK07_004571"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO8104809.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHK07_004572"
     CDS             complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHK07_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO8104810.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHK07_004573"
     CDS             complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHK07_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8104811.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGPLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHK07_004574"
     CDS             complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHK07_004574"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8104812.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHRFNVNN"
     gene            6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHK07_004575"
     CDS             6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHK07_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO8104813.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHK07_004576"
     CDS             complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHK07_004576"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO8104814.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHK07_004577"
     CDS             8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHK07_004577"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO8104815.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2240 a         2464 c         2537 g         2475 t
ORIGIN      
        1 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
       61 tccttagctg gcttctggtt gacccttcat ctaccgcgcg cgaataaaac tgccatccgg
      121 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ctgtcaccac
      181 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggttt
      241 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      301 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      361 tccgctatct ggctggaatg gttgtgtttg ggtagtttgc ggctgctctt ctggctggcc
      421 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      481 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgtgactgaa tatctaactg
      541 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      601 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      661 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      721 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
      781 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
      841 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
      901 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
      961 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1021 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1081 cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgca ctgcgtgaaa
     1141 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1201 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1261 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1321 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1381 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1441 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgtg
     1501 gcgcaggcgc gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1561 tcctctaagc agcgcattct gttcccctcg agccagaggg gagcaggcat tcagcaataa
     1621 ccccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1681 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1741 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     1801 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     1861 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     1921 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     1981 tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg ccgggccgtc
     2041 gatcattttc gtttatatga tcattggttt tatgctcttt ttcgtgatgc gggcaatggg
     2101 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2161 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2221 tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga
     2281 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg cttaacctcg ccaccgtgaa
     2341 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2401 gattgtcgtc ggcctggtca tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2461 gtcattcgcg catttgtgga atgacggcgg ctggttcccg aaaggtttaa gtggcttctt
     2521 tgccggattc cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc
     2581 tgcggaaacc aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg
     2641 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2701 ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgctgc
     2761 cgcaagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     2821 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     2881 cgctaaactt tctaagcgcg cagtaccagc gaaagggctg acgttctcgt gtatctgtct
     2941 gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat
     3001 cacgaccgtt tccgcgattc tgtttatgtt cgtctggacg attatccttt gctcgtacct
     3061 tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg atctacaaga tgccgctcgg
     3121 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3181 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cgctggggct
     3241 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3301 tgctggatgg cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgtgggaa
     3361 cagcacatta ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatgcc
     3421 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtatt
     3481 gtgtttgatc acttccagca gttcacccgc ttcatcgtgc tcgctttcca ttacgctgat
     3541 tggccccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3601 tttcatcatg tggctggaaa gctcttcatg cagcatggtc aggtattttg tcagcccttt
     3661 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca gaatcagctc
     3721 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcga tgatttctgc
     3781 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagctcagc
     3841 ttcaatgacc tcaacatcca gttctttacg tgccgccgca cgcgccagcg tttgcttacc
     3901 gccgcagcag taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag
     3961 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag
     4021 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4081 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4141 ggttttgctc tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4201 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4261 aaaacaatca gcggcccgag aatcacggtc ggtaatcgct ggctggcgac gttccagcag
     4321 agtgcgccga cccatgagca aagcacggct atcgcaatca tcagactaat aaacaccagc
     4381 ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     4441 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4501 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4561 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4621 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4681 atcagtgccg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     4741 ttggcaaaga caggaatgac caccggcagg gggccgataa tcatcgtgga aacaggcgcg
     4801 ccagtacgtt gaatggcact ggcaaggcag aagtaataaa tgaggttgcc catcatagtg
     4861 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     4921 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     4981 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5041 aacagggcgt acagcacgcc gctaatcatt actggcatcc attgatctgt cagaagaaag
     5101 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5161 atgccacttt aattaactag ttattaacat taaaacgatg gcttacgctt tcggctaacg
     5221 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gtcatcaaat agaccgcctt
     5281 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5341 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5401 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5461 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5521 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5581 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5641 tgtaaacgat gccagaatca gccaacattt tctccgtctc aatgtgctca tcggcgaggc
     5701 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     5761 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     5821 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     5881 agtcagcctg acgcacggta atgccttgtg ctgccagggc ctgggctttt gccggattac
     5941 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaagattca ataacatagt
     6001 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6061 tgagtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6121 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6181 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6241 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga
     6301 gtgaaaagat gcttgcgcag tcgttacagg cgctagagca ggatggtttt cttaaccgta
     6361 tcgcgtatcc ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg
     6421 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag
     6481 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6541 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6601 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6661 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6721 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcgcc agctatcggt
     6781 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     6841 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     6901 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     6961 atagcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7021 gcattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7081 ttcacaccat caataacatc aaagttatag gtgcggaaac cgtcccagtt gatgagtgac
     7141 tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7201 tctttcacct ctttaccgct ggctttcacc acaatcagcg tattgggata gagataaaga
     7261 tcggcggcat tacggaaagt cagctgacct ttttccacct ctacatagct tgccgggtca
     7321 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7381 tccggatcgc cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc
     7441 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7501 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7561 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgtgcttcc
     7621 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7681 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7741 gcgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatgg
     7801 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     7861 atcactttat acggatcggc agatagcccg gaatgcgcca gaacgacaac gacatcggca
     7921 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     7981 actttcccgg ataaattagc tttatcccag cccatgattt gtggcggcac gacgccaata
     8041 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8101 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8161 gggaatttcg ctcctgccag cgcatttttc aggtaatcca gaccgtagtt aaactcgtgg
     8221 ttgccaagcg ttccgacggt atagtccagc gtatttaatg ccttatagac cgggtgaata
     8281 tcgcctgctt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaag
     8341 tcgccgttat caaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8401 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8461 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8521 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8581 ttatcatcgg gaatacgaaa gaaaagggag aataaacgcc ttacttatag aacagtgaag
     8641 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8701 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     8761 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     8821 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     8881 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     8941 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9001 tagacccact ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9061 ttgcgctcat tgatcatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9121 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9181 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9241 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9301 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9361 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9421 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9481 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9541 gatttatccc aacctaataa gttataaata aaaagagatt gtatttaaag tgcaaaaatt
     9601 caattgctaa taagttacat tttaataatg agcgtttttt gatagtttac ttctataatg
     9661 agatatttaa tggcgacata aaataaccta ataaaataag gtagccatat gttacc
//
LOCUS       DAZGCQ010000156         9546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000156 DAZGCQ010000000
VERSION     DAZGCQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.156"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHK07_004578"
     CDS             complement(<1..269)
                     /locus_tag="ACHK07_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8104816.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKELQRLLG"
     gene            complement(345..944)
                     /locus_tag="ACHK07_004579"
     CDS             complement(345..944)
                     /locus_tag="ACHK07_004579"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8104817.1"
                     /translation="MQINRGLLQQFGETIRVDFQHKNDIEEAFKYASINNMVPISISD
                     SVGSMGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSGYVMSCLDNKLDDRIII
                     AGSLSKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGT
                     VSLRQNDLQQVIKYFDDKFNETEVINKGTTMPIRGEPAP"
     gene            complement(965..1381)
                     /locus_tag="ACHK07_004580"
     CDS             complement(965..1381)
                     /locus_tag="ACHK07_004580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104818.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIEKFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKNHILF"
     gene            complement(1378..2496)
                     /locus_tag="ACHK07_004581"
     CDS             complement(1378..2496)
                     /locus_tag="ACHK07_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8104819.1"
                     /translation="MTKLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVFLGTTLSISCLILALLSMFVTIIIGNNKK"
     gene            complement(2894..3316)
                     /locus_tag="ACHK07_004582"
     CDS             complement(2894..3316)
                     /locus_tag="ACHK07_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO8104820.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            3437..3715
                     /locus_tag="ACHK07_004583"
     CDS             3437..3715
                     /locus_tag="ACHK07_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104821.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(3707..3997)
                     /locus_tag="ACHK07_004584"
     CDS             complement(3707..3997)
                     /locus_tag="ACHK07_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104822.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            4236..5732
                     /locus_tag="ACHK07_004585"
     CDS             4236..5732
                     /locus_tag="ACHK07_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO8104823.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADHDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
     gene            complement(5755..6288)
                     /gene="cui"
                     /locus_tag="ACHK07_004586"
     CDS             complement(5755..6288)
                     /gene="cui"
                     /locus_tag="ACHK07_004586"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HFO8104824.1"
                     /translation="MINKHHQTDKGNKIATKMFYFLIISLIPMFSIIIIYIHNPTSNI
                     LNYIALRANDLPAIYSQNTPVFSKTLSLYVKTAPFTAILLFLNTCKNLRLKNDKSPWQ
                     LLKINIFFTFFYAILIYAFLFADTELTSSAKMLKLMSVNIILLSFFYISLHSIIFIFS
                     YLYFWFCIGSYRSFKEK"
     gene            complement(6340..6630)
                     /gene="cki"
                     /locus_tag="ACHK07_004587"
     CDS             complement(6340..6630)
                     /gene="cki"
                     /locus_tag="ACHK07_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFO8104825.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDTDNTPLLFIFISSIYVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLIAIPVGLPSFIYYSLKRK"
     gene            6660..6791
                     /locus_tag="ACHK07_004588"
                     /pseudo
     CDS             6660..6791
                     /locus_tag="ACHK07_004588"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
     gene            complement(7011..7366)
                     /locus_tag="ACHK07_004589"
                     /pseudo
     CDS             complement(7011..7366)
                     /locus_tag="ACHK07_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            7432..7656
                     /locus_tag="ACHK07_004590"
     CDS             7432..7656
                     /locus_tag="ACHK07_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO8104826.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            7653..8039
                     /locus_tag="ACHK07_004591"
     CDS             7653..8039
                     /locus_tag="ACHK07_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO8104827.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            8559..8813
                     /locus_tag="ACHK07_004592"
     CDS             8559..8813
                     /locus_tag="ACHK07_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO8104828.1"
                     /translation="MKITTLSSRELNQDVTRAKRATSYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            8813..9229
                     /locus_tag="ACHK07_004593"
     CDS             8813..9229
                     /locus_tag="ACHK07_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO8104829.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
BASE COUNT         2783 a         1733 c         2067 g         2963 t
ORIGIN      
        1 ttacccagca agcgctgtaa ctcttttatt tgttttatag cggcagccag ttctgaggca
       61 ggaacgacat cctctccggc agcaacagca gtgagacttc cttccagata ttgttttttc
      121 cacttaaaca acaggctggg ctgaatacca tgcaaacgcg ccacatggga caccgtcata
      181 ccgggttcca ttgtctgctg aataatggcg attttttcct gaggagtttt acgtttacga
      241 agctcctgcc ccagcaaaat tccagtcatc tcaaaattag cactagtgtt agacatatat
      301 ccaagcctat ctcttatttt aagatacatc cactgtctgg cgtttcaagg ggccggttca
      361 ccacgtattg gcattgtagt ccctttgttt atgacttcgg tttcattaaa tttgtcatca
      421 aagtatttta taacttgttg taagtcattt tgacgcaagg atactgtgcc atcgaggtgt
      481 aaatctgctg ctgcgataca tgcagcaatt cctggtaatg atggtggacc accaaaagca
      541 taggtagttc catacgtttt tataaagttt atggtgtcag gataattgca tgcaataaat
      601 ccaccatgag agccaaacgc tttagataat gaaccagcga taataattct gtcatcaagt
      661 ttgttatcca gacaagacat tacatatccg gagccatttt tcccatatat tgacatgcca
      721 tgagcatcat caatgtaagc atacccatca aatctgttag ccattctaac aataaattcg
      781 acaggagcca ccccacccat cgaaccgaca ctatcagata tagatattgg taccatatta
      841 tttattgaag catatttaaa cgcttcttct atatcatttt tatgttgaaa gtcaaccctg
      901 attgtttcac cgaactgttg taatagtcct ctatttattt gcaatgttgc atgcgctgtt
      961 ttctctaaaa taaaatatgg tttttattaa acttgtaatt aggtagtgtt cctgatgcaa
     1021 gaagtgggag tacagctaaa tggcatggag taaccgcatt aaatgttaat gtgtgcgccc
     1081 cattcattat tttatttaat ctcttttcca attgctcgaa taaatctact tttacgcgcg
     1141 ttctggcgac cgaaagttgg actccaaatt tttcaattgc attagcgatt gcatgtttta
     1201 gattttcatg tgtttccagt ccaagataag agcatgacac aaactcaatc atctccctcc
     1261 catcattaac agtgattacc ttaccatttc ttctggtgat cactagttca tttatatgtt
     1321 cgttaaatac tcttgtttgg tatttgattg aacgttctat ctttttcttt tgccaattca
     1381 ttttttatta ttccctataa taatagttac gaacattgac aataatgcaa gaataagaca
     1441 actaatactt aaagtggttc ccaaaaaaac tccattatcg cttattttta ctgaaaggaa
     1501 actgattgtc cctgcgctta ctgtttgagt aaagcctgta attgatgctg ctaaaccagt
     1561 tcgtgaaccg tttatctgca atgagatgaa attagctatt ggatagtatg cgccaagagt
     1621 taaattgaaa atcattgctc caataacaca tattataaaa gcatcattta atataagaaa
     1681 aggtgtaact gatattgcat ttaggggaaa taagacccat gggtttgata ttttttcgat
     1741 ttttgacaat tggtttccaa tgatgaaaaa caaaactgac agggaaaaaa caacagagta
     1801 gtgaaatgta tttaatccat aatcactcat aaatagtgca ggtgatattg atatgtagca
     1861 aaatagtgag cttagcatta gtgaaacgag tattgtcccg cagatgaaat ctttagtcac
     1921 aaattttaca ttatcttgat gtgttttttt ctcctgttta ttagtctctt taggtgacag
     1981 gagtatggtt agaatgctaa atgcacccat tacaataaat attacattcc agttataata
     2041 atcaagtatt aatgctccgg ccagaggtgc tattaatggt attatcgcac ttgttgatga
     2101 tatgtcagaa agtgctcttt ttagctttag tggtggaaaa gactctttag caatggcttt
     2161 tgatattaca ggtccggcac ctgcacctat tgcctgtaga attctaccca aaagaagcat
     2221 atttaaatca ttggccataa aacatacaat actgccaata agaaatataa ttaagcatat
     2281 agtcattacg cgtacttttc cgaaataatc acctatggtt cctgatatca gttgactaaa
     2341 tgcaaataca ataagaaatc cactaatact tagttgaatt aatgagtagt cagcatgaaa
     2401 tgctttctgt agttctggta acgcaggcac aatcatgtca gttgataatg ccgataatga
     2461 tgcagcaaga acaaggtaaa tatatagttt tgtcataatt aatccctgat aaaaatattc
     2521 ttatttatca tatttaaaat gcttacgcca tcggtatgtt ttatattttg agatagcact
     2581 acgatagtgt ttgagtagaa actaatgggt actcccagat gcatattcag gttgatggaa
     2641 gggggtaaaa atccccgtgc tgaaccttcc atccggaggg aggaaactta tcattttaac
     2701 ctgttcgtcc gtcggaatag tcgaaatcga tcgcgcatcg cttttgtgaa tagttatgca
     2761 agtacctgaa aacgattctg acgcattttt tcggtttagc ctggggtttc cttgtctttt
     2821 tgcgtttttg cgtcagaacg cgtatgagag cgtttcagcg gtacgtacaa ttaagggatt
     2881 atggtaaatc cccttactgt ctgccctcgt atccatcgag ataaaccgca gaaatggtgt
     2941 ctgccattag tttcattttt ttcggcgacg gcatatcgct gtttgtgtat tttctgccag
     3001 caaaggttgt tgtgctccgg agtgagtgaa aaaatttttc tgagtggaat tcttttatac
     3061 gttgctgtgc cgtttctgct gatactccgg ctgctttgtc tttcaccccc atatcccgga
     3121 actgctgaaa gaacgggagc atcatacgta tggtgtctgc tttctgggtg tagaagttag
     3181 gatcaaatcc atcatctgaa gcataaacat aatgccgggt atgtcgttcc aggctggtct
     3241 ttggtgtgca tcctgtaaga acgattgttg ctgttatcag aggtatcaga agcagcctgg
     3301 caacggcaga tctcataaac atctccttgc gtttttaatc ttgatactca gggagttata
     3361 tttccgaaga gtatctctta tcatacgtcg gtttacccca atgtgatttg atggtcaatg
     3421 agtcaggaat aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg ttctttctgc
     3481 ttcggttgcc agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga agacaaaaaa
     3541 tggcattgtt gcggatgtta atgccagaca gaaaatgttc agtaacagac tgaagataca
     3601 acttcagatc gatgagttga gggcatctct ggctggcacc agagatgccc aaaaaagaga
     3661 ggatttgcgt aaaaaactgg agtctctgga gcggcaaaag aaatccttac gctgatgggg
     3721 gcgtattttc cccggtttct tccataacct ttttaccgtt cagatattta cgggcgaggc
     3781 gaatgcgtct tgcctggtat accgccgagt aagcctgggt gggattcaca ccgttactga
     3841 tttcactgac cacatggcgc tgatcagcca gtgtcagcgt gtaaacctgt ttctggatga
     3901 ggtcacgggc tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga agtcgtttgt
     3961 ctgttttgtt cagtttatgg cggggtgatt tgctcacatc gttctccgtt tttcatgatc
     4021 caacagtttt atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc
     4081 tgcctcccca tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca
     4141 atggtgagta gtaggttttt ctgtacataa aaccagtggt tatatgtaca gtatgttgtt
     4201 tttaatttat tgttttaatt atcaaagagg aatttatggc taaagaacta agtggatatg
     4261 gcccaactgc tggtgagtct atggggggaa caggggcgaa tctgaatcag caaggtggaa
     4321 ataataatag taatagtggg gttcactggg gtggtggttc aggctctggt aatggaggtg
     4381 gtgaacatgg aagtcagacg gggtggggat ggagtaaaac caataatccg gatgtgccgc
     4441 cttatgttga tgataatggg caggtgcgca ttaccattac aaatggtctg gtaaaaacgc
     4501 ctgtatatgg agttcctgag gctggcggaa atagtgatgt tcaggggggc tatattcctg
     4561 aaaatcctaa tgatgaagtt gctcgtaagt gggataaaaa caacttaccg cgagagattg
     4621 atgtgagtat cgatggtttt aaatatcgtg taacgcttaa tgataatggt agagctatcg
     4681 gtattcttcg aacaggtgtc cgtccttacg tagggagtga aaaagctaag gcgggtatta
     4741 tggaaaaaat caatcataaa acacctgagg agatatatga ggcgttaggt tttaataaag
     4801 atgaatctca gcggcaggaa aaagcaaaac aacaggctga agatgcgtgg gatcgtttgc
     4861 ctcctaatgt gagaaaattt gatgttgatg ttgaacagtt ccactattta gtggtgcttg
     4921 atgattatgg taatgtttta tctgtaacta gaacagctgt tcgtccttat gtagggagtg
     4981 aaaaagctaa ggctggtatt atggataaag tcgaccataa gaccccggaa gagatatacg
     5041 aggcattagg atttaataat gaagagcctc aacgacagaa tcaagctaaa aaagctgctt
     5101 atgatgtatt ttattctttc agtatgaacc gtgaccggat tcagagtgat gtgctaaata
     5161 aagcagctga acttatatct gatatgggta acaagatcgg tgattatctt ggtgaatctt
     5221 ataagtcgtt agcaagagaa attgctgatg atatcaaaaa tttccagggg aaaaccatcc
     5281 gtagctacga tgatgcaatg gagtcattaa ataaaatttt gtctaatcct agttttaaag
     5341 ttaacagagc agaccatgat gcactggtga acgcctggcg aagtcgtgat gctcaggata
     5401 tagcaaataa actggggaat atcagtaagg ctttcaaagc tgccgatgta attatgaaga
     5461 ttgagaaggt caaggagaaa agcatcgagg gatatgaaac tggaaactgg gggccgctga
     5521 tgttggaggt tgagtcttgg gttctcagtg gtatggccgc aagcgttgcc atgacattgc
     5581 ttagtgctat aattggatca ttcacactac cggcgttagc attaactgca ataaatattg
     5641 caggtattat gggaattagt taccttgcat catatattga tgcaagtgtg gctgaaaaaa
     5701 taaataataa attggttcat tttactcatt gatagaaaaa gagaggtgat catatcactt
     5761 ctctttaaac gacctatagg aaccaataca aaaccaaaaa tacaaatatg agaatataaa
     5821 aataatggaa tgtaagctaa tgtaaaaaaa cgataggagg attatattta cagacatcag
     5881 ttttagcatt tttgcagaac tggttagctc cgtatcagca aataaaaaag catatattaa
     5941 gatagcataa aaaaaagtga aaaaaatatt tattttcagt agttgccatg gagatttatc
     6001 attttttaat ctaagatttt tacatgtgtt taaaaataaa agaatggcag tgaaaggagc
     6061 tgtttttaca tacagactca atgttttgct aaatacgggg gtgttttgtg agtatatggc
     6121 aggtaggtca tttgctctca atgctatata gtttaatata ttggatgttg gattgtggat
     6181 atatataatg attattgaga acattgggat tagtgaaatt ataaggaagt aaaacatctt
     6241 tgttgctatt ttatttcctt tgtctgtttg gtggtgttta tttatcatct gtttacctgt
     6301 aaatttatta tattaaagat tttgatgaat ggctatggtt tattttctct ttagagaata
     6361 atatatgaaa cttggcaatc ctactgggat tgctattaat aaatagaaca atgcagataa
     6421 cttgcataag taccagggct ctttttttaa ctttgtattt tctttgatat atctagatat
     6481 ggttaattta gagtatggat atagcaatac atatattgac gatataaaga taaataacaa
     6541 aggagtgtta tcagtgtcgt taaatattaa aataagaatc catggtataa gtgattcagg
     6601 taaattatgt aggtagtatt ttaaatgcat aagtattttt acttttgtcg gaaagggata
     6661 tgaaaataat aatttttcta attaaaatat tattcttgtc aggatgtcaa gcaaactata
     6721 ttcgtgatgt acaatgagga accgttgcgt catcttcgtc ttctgaactg acggggatcg
     6781 cggttcagta gaaaagatca aaggatctct tgagatgggg ttccaagccg gaaacctgga
     6841 tttttccgcg accagtaacg gcgtggggtg tcacataaat gaccgattga gtgactgtca
     6901 ctgaaacaac gatttctaca tcagatttcg tcacataacc cgtcacctat tggggtcagt
     6961 ttggatatag agaattaggg ggctggcgct cagtggaacg aaaactcacg ttaagcaatg
     7021 ttttcttcct ccgatgcctc tttttgccct ttcgtcacca gttctgccag cgtgaaggaa
     7081 tatggccgag catattgatg tgtccgtggc aaagtggtga gaggcgtgcg atatcttcat
     7141 cattcacgat ttccccctgt gccctgaaat ggtccagggc agcctgcata taaatagtgt
     7201 tccataatac gacggcgtta gtcaccagcc ccagtgcgcc cagttgatct tcttgaccat
     7261 cggtatatcg ctttcttatc tcacctttct gaccgtgaca gatagctctg gcaacggcat
     7321 ggcgactttc tccccggtta agttgggtca ggatgcgcca gcggtaacgg tgatttcata
     7381 ttgaagataa ctgttgtaca atttaattgt acaactcaaa gaggaagcac tatgtttaca
     7441 tgtaactata gccatgctcg cgataacctg gcctcgttgc tcgagctggc ggttgatgaa
     7501 aatcagcctg ttgagattca gcgccgtaaa ggtggcgcgg ctgtactggt tgataaagac
     7561 gcttttgaac gataccgtaa agcggctctt gatgctgagt ttgctgagat catgcaaagt
     7621 catgggacgg cctatacgga gctttctaag cgatgaaatt cgttagttca caggaaatga
     7681 ttgagtttca tgaccgcttg atttcgcgtg atggtggcgt ggcgggtatg ccagaacccg
     7741 gtcgcgctga cgcgatcatt catcgtgttt tgaacatgta tcattatgaa ggtgtgacag
     7801 atattatgga tctggctgcc gtgtatttag ttgctattgc tcgtggtcac attttcaacg
     7861 atgcaaataa gcgaacagcc ttgtttgttg cacaggtttt tttaaaacgg aatggtgtac
     7921 atattattag ttcacgtatt tcgtttgatg aaatgcaaat tatcgctttg aatgctgcaa
     7981 caggagaata taactggaaa atggtttctg accatcttaa agccattatt ctaaattaga
     8041 tatagatatc caatcagcag cattttacaa tctactgagc aatgtaaaaa tcatatatct
     8101 ttttactata taatttctgg cgtatgtgct caattcagac ctgacactct tccgcttcct
     8161 cgcccactcg ctcactaacg ttcggtcgct ggggctgcgg cgagcgtttg taagttttct
     8221 ccaatactga cgacgtgctt cctgatggat aataatgtgt aataatacac attattactg
     8281 tattaagtct taaatggggt ccgcttggaa ttcggaaaat aaaccacgtt aagactgttt
     8341 tttagccaac agactttcag tttagattat ccgatgaagt tccagcgtag aacctcatcc
     8401 aggacgtggc cattaatgct aatgtatgca tacattagca ttaatatctg acagcaacaa
     8461 gcggcgaaga ctacccctaa tcaagtacat ttcttgcatt gtactatttc atgggccaca
     8521 atgtagtaac atacctatat gaatcaaaag gcaacagcat gaagattaca actctatcca
     8581 gtcgggaact gaatcaggat gtaacccggg ccaaacgcgc cacaagttat ggtcccgtat
     8641 ttattactga tcggggtaaa accgcacatg tacttcttag tatcgaagaa taccgaaaat
     8701 tgacgcaaca acgacgaact ctcattgaca ttctggcgat gcccgatgct gatgcagttg
     8761 aatttgaacc gcctcgtatt acggttagtt taaaacctgc ggagttttta tagtgtatat
     8821 tcttgatact aatgttatct ctgagctacg gaaggctcgg acaggacgta tagataataa
     8881 tgttgcaaaa tgggcattgc atacagatgc ggcagatttc tttatttctg ttatttccat
     8941 aatggaactt gaacagggaa tattacaagt tgaacgtcga gatatgcgac aagggattat
     9001 gctacgttcc tggatggatc agcatgtgtt accagcgttt tctgataaga tacttcctgt
     9061 agatactgcg gttgcattac gttgtgctcg tttacatgtt cctgacaggc gcagtgaacg
     9121 agacgcattg atcgcagcaa cggctctggt acatgggatg acggttgtaa ccagaaatgt
     9181 tggtgatttt gaattaaccg gagtatcaat tattaatccc tgggcttgat atcgcttccc
     9241 ttcttctagg gctccgtttg ggaaacggaa tcgtactata agagcgtaag gtgtcaggtt
     9301 gaagtcacag actccgcgtc aggagtgtcc agaaaattcg aaatagaccc tattctgacg
     9361 tgtttcgccc aaacaacctg acgtcagact gaagtacact ccaacctgac agcagtatga
     9421 gcaacaacaa gaatggcgca gctttggctt cgatagctgt ctcttagcgt acaaaaatat
     9481 ccgtttcgtg agctgcccca tattgtacgt ttcccaaacg cagccctaat aaagtcattc
     9541 gatggc
//
LOCUS       DAZGCQ010000157         9481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000157 DAZGCQ010000000
VERSION     DAZGCQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.157"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            276..638
                     /locus_tag="ACHK07_004594"
     CDS             276..638
                     /locus_tag="ACHK07_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104830.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(919..1377)
                     /gene="ybbJ"
                     /locus_tag="ACHK07_004595"
     CDS             complement(919..1377)
                     /gene="ybbJ"
                     /locus_tag="ACHK07_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO8104831.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1374..2291)
                     /gene="qmcA"
                     /locus_tag="ACHK07_004596"
     CDS             complement(1374..2291)
                     /gene="qmcA"
                     /locus_tag="ACHK07_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO8104832.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2437..3114
                     /gene="fetA"
                     /locus_tag="ACHK07_004597"
     CDS             2437..3114
                     /gene="fetA"
                     /locus_tag="ACHK07_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO8104833.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3101..3880
                     /gene="fetB"
                     /locus_tag="ACHK07_004598"
     CDS             3101..3880
                     /gene="fetB"
                     /locus_tag="ACHK07_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO8104834.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3943..4797)
                     /gene="cnoX"
                     /locus_tag="ACHK07_004599"
     CDS             complement(3943..4797)
                     /gene="cnoX"
                     /locus_tag="ACHK07_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO8104835.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4858..5667)
                     /gene="ybbO"
                     /locus_tag="ACHK07_004600"
     CDS             complement(4858..5667)
                     /gene="ybbO"
                     /locus_tag="ACHK07_004600"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8104836.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5657..6280)
                     /gene="tesA"
                     /locus_tag="ACHK07_004601"
     CDS             complement(5657..6280)
                     /gene="tesA"
                     /locus_tag="ACHK07_004601"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO8104837.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6251..6937
                     /gene="ybbA"
                     /locus_tag="ACHK07_004602"
     CDS             6251..6937
                     /gene="ybbA"
                     /locus_tag="ACHK07_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO8104838.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6934..9348
                     /gene="ybbP"
                     /locus_tag="ACHK07_004603"
     CDS             6934..9348
                     /gene="ybbP"
                     /locus_tag="ACHK07_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO8104839.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMINLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LSEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMHQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2163 a         2424 c         2602 g         2292 t
ORIGIN      
        1 cgaaatctac gccgccattt gtgtgatgta aagcgcgttt ttgtcttgtg taagcgtaaa
       61 cactgtctat aagaaactga attggtttct taagtaaata attttgaaaa tcattttgtg
      121 ctattttagc gtaaaaaaga gtaaagactt gttttgataa gcgaaataaa ttctgtttct
      181 ggactaaacg tgaagcggtt cctgatgtgt gctaaaatgc gtttctttga taaatctcaa
      241 tttttaagag gtttacgcca ggcaggaaaa tttaaatgaa tgaatttgag aagattttta
      301 atgaaatgaa tctggaccgg gcattgctac cgatcctttt tcgttcgaac cggtcgacag
      361 tgtggaagta tttaagcggc gatagtacgg ctcctgcctc tgccatgtcg ctgattatgc
      421 tgttgcagtt gatccagaaa cgaaaccccg atctgttagc cgaatggtta acgctttctg
      481 atttcaccat tccgccagaa gtttacctgg accagcctga ttactggaaa gggtgggtgt
      541 atacgcaaca caaggtcaat aaaaacgtcc tggaatatct aaaaaaacac tatccggatg
      601 aagaccaaaa aggtatgggt aaaaccacag aagagtaaat tgtcattacc cgcccacatg
      661 ccggatgcgc acagactggc agtgttagta ggcatgataa tacgcgtcag cgtcgcatca
      721 ggcatcggcg cataaccgcc ggatgcggcg taaacgcctt atccggccta cgattcgggc
      781 gcagacttcc aggcatgata atacgcgccc gcctcgcatc aggtgtcggt gcacgactgc
      841 cggatgcggc gcaagcgcct tatctggcct acgattcggg catattttgt aggcatgata
      901 agacgcgtca gcgtcgcatc aggcgatgac cgcacggatg atcagcgtta tcccttctat
      961 cgcaatgact tcaacatgcg tacctgcgcc gagatcctcg ctggcgctga caggccatga
     1021 actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca gcacaaaacg
     1081 tcggccaatc agctgctgcc cgcgctggtt taaatgactg tcgctgtgct tttgttcgcg
     1141 cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca ggacggcaaa
     1201 catcacccct tgccactccc aacccagcgg caccagccag accaccaggc cggtaatcac
     1261 cgctgccacg ccactccaca acaaataacc atttccgccc agcatctcgg ctgccagcag
     1321 caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact ccatcatggc
     1381 tgagtccgtt tgttggcgct gtctttcacc agctcagcaa tcccggcaat cgaccccatc
     1441 aggctgctgg cctctaatgg catcatcact actttgctgt tactggacga accgatctgc
     1501 tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat atcaccggag
     1561 gcgatggctt cagacaccat tttggtggcg cgggcttctg cttcagcgga acgttcacgc
     1621 gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat ttgcgactgt
     1681 ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc aagaatgtaa
     1741 gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag ctctgccggt
     1801 gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt ggtggcttca
     1861 tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt
     1921 tccattgaac ccagcacggt acggatgtta gtcatggtca ggttgatgat cgccagctcc
     1981 agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa acagactgcg
     2041 tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat atcgagcact
     2101 tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac cagactgagc
     2161 cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg atagccctgc
     2221 ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag aatcgggata
     2281 aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa ttgctgcttg
     2341 attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg cgaaggataa
     2401 actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag tcctttgctt
     2461 cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa catcaatttt
     2521 tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg caaaagtacg
     2581 ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact gtttgaaggt
     2641 gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta ctgcgcccag
     2701 acaccgacgc tgtttggcga tacggtatac gataatctga tctttccctg gcagatccgt
     2761 aaccggcagc ctgacccggc catttttctc gattttcttg aacgcttcgc cttgccggac
     2821 agcattttga cgaagaatat cgccgagcta tctggtggtg aaaaacaacg catctcattg
     2881 attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaataac cagtgcgctg
     2941 gatgaaagta ataaacataa cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat
     3001 attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga taaagtgatt
     3061 acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc ataatattac
     3121 taacgaatca ttagcactgg cattaatgct ggtggtggtg gcaatcttaa ttagccataa
     3181 agaaaaactg gcgctggaga aagatattct ctggagcgtc gggcgcgcga taattcagct
     3241 gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca gcctgacatt
     3301 attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata
     3361 tattgctaaa gcttttatct catcgtttat tgccattacg gtcggggcgg gaattaccct
     3421 ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga tccctatcgc
     3481 cgggatgata gccggtaacg ccatggtagc ggtagggctg tgttacaaca atttaggaca
     3541 acgggtgatt agcgaacagc aacagattca ggagaaactg agtcttggcg cgacgccgaa
     3601 acaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc cgacggtaga
     3661 ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc tgatatttgc
     3721 cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta tgctgctctc
     3781 aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt tttataattc
     3841 gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg gatgcgcagc
     3901 atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa tatcaataca acaatgcata
     3961 cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag cgaggatctc
     4021 ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa
     4081 cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg ccagttgcgt
     4141 cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt gaatttccgg
     4201 cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt agcgggtgtc
     4261 ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac ggttcagcgc
     4321 aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca actgccaggc
     4381 gtctttcagc aatggcaggg cgtcggtgta attgccttcc tgcatcagtt gcatcgcctg
     4441 ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc ggatcgcctc
     4501 ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca gatacacggt
     4561 cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc agtccagctt
     4621 cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccagaattg gggttaactg
     4681 caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg tcatcgactg
     4741 ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta cggacatgga
     4801 gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg cttcaactca
     4861 cccctgcaat attttgtcca tcacgcgccc cggcagcagg cgcttaagca ccattactgc
     4921 ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa aagcatggcg
     4981 tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc
     5041 gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac gaatgggacc
     5101 gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca gcgcgtctga
     5161 ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg tggagattaa
     5221 tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa
     5281 caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct gttccatctg
     5341 cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt taaagatccc
     5401 atacagacaa ttatcggtca gggcgatcac ctcgtcggct gcgcgatcaa cactttctgg
     5461 tgaatccaga tcgatcaaca cgccggtaaa tcccatgctg ttcatgcgct caacatcatc
     5521 cggtttccgg caacctgcca gcacatgaaa accctggcgt tttaattcga gcgcgctttc
     5581 caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa ctttacctgt
     5641 caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc
     5701 agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt
     5761 gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca aactctttgg
     5821 cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag tttgcaggca
     5881 gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc aaaatctggc
     5941 gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg ccgcccagtt
     6001 caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc gccagccctt
     6061 gttgcgaggt gtcgccgctg atgctggcat tgactaccga cgttttactc tgccacttat
     6121 cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat
     6181 cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg accaggaaca
     6241 ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg
     6301 tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga
     6361 gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc
     6421 cgggctggat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat
     6481 ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt
     6541 tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggcgc tgctgcgcgg
     6601 tgagagtagc gcggaaagtc gtaacggagc gaaagcgttg ctcgaacagt tagggctggg
     6661 taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac gagtggcgct
     6721 ggcacgagcc tttaacggtc gacctgatgt gctgtttgcc gacgaaccca ccggtaacct
     6781 tgaccgccag acgggcgata aaattgccga cttgctgttt tccctcaacc gtgagcatgg
     6841 caccacgttg ataatggtga cccacgacct gcaactggcg gcacgctgcg accgctgctt
     6901 acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt ctggcgcgaa
     6961 tggcgctcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg
     7021 ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca aagccgtgag
     7081 tttatggcgg gcgatcgggc gctgcgcagt tcgcgcgaag tgccgcaagc gtggctggag
     7141 gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac catgacattt
     7201 gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat
     7261 ggcgatctgc aaaccaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc
     7321 ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt gggcgatgcc
     7381 accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa ccccttccag
     7441 atggctccgc gtctgatgat taatctggcg gatgtcgata aaaccggagc cgtgcagccg
     7501 gggagtcggg tcacctggcg ctataaattc ggcggcagcg agaaccagct cgacagctat
     7561 gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct ggaacaggac
     7621 gaaggcgcgc tggggcgatc gatggaacgc tcgcagcagt tcctgctgct ttcggcgctt
     7681 ctgaccttgc tgctggcagt ggcagcggtg gcggtggcga tgaatcatta ctgtcgcagt
     7741 cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag
     7801 ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg tggggcgata
     7861 ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta
     7921 ccgccagcca gcctctggcc atggctgtgg gcgctcggca ccatgacggt catctcgctg
     7981 ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg cgtattacgt
     8041 aatgatgtgg ttgcgaacgt ctggccgctg aagttttatc tgccgattgt cagtgtggtg
     8101 gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc ggtgctggcg
     8161 ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgctgct gaatgtactt
     8221 cgccgtatga cgctgaaatc gctgcctctg cgcctggcgg ttagccgcct gttacgtcag
     8281 ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct gctggcactg
     8341 ctgctggtgt tgcgtggcga tctgctcgac cgctggcagc agcagctacc tccagaaagc
     8401 ccgaactact ttttaattaa catcgccaca gaacaggtag caccgctaaa agcgttcctc
     8461 tcggaacatc agatagtccc ggaatcgttt tatccggtgg tgcgggcgag actgacggcg
     8521 attaacggca agccgacaga aggcaatgaa gatgaggcgc tcaatcgcga actcaatctt
     8581 acctggcaaa atacgcggcc cgatcataat ccgattgtcg ccggtaactg gccgccaaaa
     8641 gccgatgaag tgtcgatgga agaggggctg gcgaaacgct taaacgttgc cctcggtgat
     8701 accgtgactt ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa
     8761 gttgactggg aaagtctgcg gcctaatttc tattttattt tccctgaagg ggctctggac
     8821 gggcaaccgc agagctggct taccagtttc cgctgggaga atggcaacgg catgttgacg
     8881 caactcaacc gccagttccc gaccattagc ctgttagaca ttggcgcgat tttaaaacag
     8941 gtcggccagg tgctggagca ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc
     9001 gcctgcggta tgttgctgtt gctggcacag gtgcaggtgg gaatgcatca gcgtcatcag
     9061 gagctggtgg tgtggcgcac actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg
     9121 tgcgagttcg ccatgcttgg gtttgtttct ggcctggtgg ccgcaattgg tgcggaaacg
     9181 gcgctggcag tgttgcaggc gaaagtgttt gatttcccgt gggagccaga ctggcgattg
     9241 tggattgttc tgccgtgcag cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggc
     9301 gcgagactgg ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg agagtaaata
     9361 aggggcgttc tggttgaatg gaacgccttg tttaaattaa ttctacaata tgtttcattg
     9421 atgatgttat tgaattggtg atttcctatc tttctattgc tgatattaat ctgaatcggt
     9481 g
//
LOCUS       DAZGCQ010000158         9477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000158 DAZGCQ010000000
VERSION     DAZGCQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.158"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..93
                     /gene="tnaC"
                     /locus_tag="ACHK07_004604"
     CDS             19..93
                     /gene="tnaC"
                     /locus_tag="ACHK07_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO8104840.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            314..1729
                     /gene="tnaA"
                     /locus_tag="ACHK07_004605"
     CDS             314..1729
                     /gene="tnaA"
                     /locus_tag="ACHK07_004605"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO8104841.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHK07_004606"
     CDS             1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHK07_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO8104842.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHK07_004607"
     CDS             3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHK07_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO8104843.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSQAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHK07_004608"
     CDS             4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHK07_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO8104844.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPSGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5465..6214
                     /gene="yieE"
                     /locus_tag="ACHK07_004609"
     CDS             5465..6214
                     /gene="yieE"
                     /locus_tag="ACHK07_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO8104845.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6236..6802
                     /gene="yieF"
                     /locus_tag="ACHK07_004610"
                     /gene_synonym="chrR"
     CDS             6236..6802
                     /gene="yieF"
                     /locus_tag="ACHK07_004610"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO8104846.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQEEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTNSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHK07_004611"
     CDS             complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHK07_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO8104847.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8360..9025
                     /gene="yieH"
                     /locus_tag="ACHK07_004612"
     CDS             8360..9025
                     /gene="yieH"
                     /locus_tag="ACHK07_004612"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO8104848.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEHGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASVNV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9162..>9477)
                     /locus_tag="ACHK07_004613"
     CDS             complement(9162..>9477)
                     /locus_tag="ACHK07_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO8104849.1"
                     /translation="KDYDDLLREIKELTISRNKSLEDKRTLFDNKRDNWNSIEVQDEV
                     NALNAKIVDCDDKIRSKLTIIRNNRLENQYKDDKNISDAMRNILDWFEQYSDIVKNIT
                     KA"
BASE COUNT         2335 a         2352 c         2335 g         2455 t
ORIGIN      
        1 ttcgaggata agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat
       61 attgacaaca agattgtcga tcaccgccct tgatttgccc ttctgtagcc atcaccagag
      121 ccaaaccgat tagattcaat gtgatctatt tgtttgctat atcttaattt ttccttttgc
      181 aaaggtcatc tctcgtttat ttacttgtgt tagtaaatga tggtgcttgc atatatacat
      241 ggcgaattaa tggatattgc aaatgtaata ttcacaggga tcactgtaat taaaataaat
      301 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      361 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      421 gaacccgttc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      481 cggggcggtg acgcagagca tgcaggctgc gatgatgcgc ggcgacgaag cttacagcgg
      541 cagccgcagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      601 cattccgact caccagggcc gtggcgcaga gcaaatctat attccggtac tgattaaaaa
      661 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt
      721 tgataccacg cagggccata gccagatcaa cggctgtacc gtgcgtaacg tctatatcaa
      781 agaagcattc gatacgggcg tgcgttacga ctttaaaggc aactttgacc ttgagggatt
      841 agaacgcggt attgaagaag ttggtccgaa taacgtgccg tatatcgttg caaccatcac
      901 cagtaactcc gcaggcggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      961 cgcgaagaaa tacgatattc cggtggtaat ggactccgcg cgctttgctg aaaacgccta
     1021 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1081 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
     1141 gggcggcttg ctgtgcatga aagacgacag tttctttgat gtgtacaccg agtgcagaac
     1201 cctttgcgta gttcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
     1261 gcgtctggcc gtaggtctgt atgacggcat gaatctggac tggctggctt atcgtattgc
     1321 gcaggtgcag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1381 cggtcacgcg gcattcgttg atgccggtaa actgttgccg catatcccgg ctgatcaatt
     1441 cccggcacag gcgctggcct gcgagctgta taaagtcgcc ggtatccgcg cggtagaaat
     1501 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggcaga
     1561 actactgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1621 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaagggttaa cctttaccta
     1681 cgaaccgaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
     1741 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
     1801 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
     1861 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
     1921 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
     1981 tcattccggg ttattgttat tagaagcaaa tttaaattat cccgtcggct ccagttttaa
     2041 caccatcacc aaagatttaa ttggtaacac ctggaacatt atcagcggta ttaccgttgc
     2101 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcaa tcattagtga
     2161 aacgatatca atgaatttgg gctatcacgc taatccgcgt attgtcggga tctgcacagc
     2221 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgta ttacctcatt
     2281 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     2341 cgattactcc attctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     2401 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     2461 cctgattatt tgctatggca aacgcaaaga taagctaatc aaaagcgtgg tcttcggttc
     2521 gctgctggcg ctggtgattt atctcttctg gctctattgc accatgggga atattccgcg
     2581 agaaagcttt aaggcgatta tttcctcagg cggcaacgtt gattcactgg tgaaatcgtt
     2641 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ccaacttagc
     2701 tgtcgccagc tcgttttttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     2761 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     2821 cgcgttgttg tatctgatct tcccgaatgg ctttatttac gggatcggcg gtgccgggct
     2881 gtgcgccact atctgggcgg tcattatccc cgcggtgctg gcaataaaag ctcgcaaaaa
     2941 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     3001 ctttggtata accgtaattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     3061 tggctaaatc cttcaagaag ccagtcattc gctggcttct tgcctctcag gaaatcactt
     3121 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     3181 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     3241 tatcccgctg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     3301 gccagcgaag cgcagttaca tattgcgttc tccgtttatc tggcaggtat ggcagctgcg
     3361 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa aaccggtcgc catccccggc
     3421 gcggcgctat ttattatcgc ttcggtgttc tgttcacagg ctgaaaccag tacgttattt
     3481 cttgcagggc gctttctaca ggggttgggc gcgggctgtt gttacgtggt ggcattcgcc
     3541 attttgcgcg acacgctgga tgatcgacgt cgggcaaaag tgctgtcgtt actcaacggc
     3601 attacctgca tcattccggt gttagcgccg gtgctcggtc atctgattat gcttaaattc
     3661 ccgtggcaga gtctgttctg gacgatggca ataatgggca tcgcggtact gatgttgtct
     3721 ttgtttattt taaaagaaac gcgccctgcc gcccccgcag cttcggacaa atcacgagaa
     3781 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     3841 gtttcggtga tcctcacctt cgtcaacacg tcgccggtat tgctgatgga aatcatgggt
     3901 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     3961 tcattctcga cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctca
     4021 caggtgttat tcctggcggc ggggatcact cttgccgttt caccttccca tgcggtttct
     4081 ctgtttggta tcacgctgat ttgcgccggt ttctcggttg gttttggtgt ggcgatgagt
     4141 caggcgttag ggccgttttc attacgcgcg ggtgtagcca gctcgacctt aggtattgcg
     4201 caggtttgcg gttcgtcact gtggatttgg ctggcggcgg tggttggtat cggcgcatgg
     4261 aatatgctga tcgggattct gattgcatgt agcatagtga gcctgttgct gattatgttc
     4321 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     4381 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     4441 gatgaacgtg accccttcgg cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     4501 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     4561 ggagcaaaat ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcatca
     4621 ccagacaccg agcggcctaa agtttgagct ggcggcagaa tcaccgctaa tgatgatcat
     4681 gcttaatgcg ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg
     4741 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata tcggtttttc
     4801 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     4861 ttatgaagtg ctgtttagtg atgtgccttg cgtctggtta cgcaaagatc atccggcact
     4921 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     4981 acagagcgat acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     5041 tgctatgagc ctgccggaat tcgagcagtc gctgtttatg gcagcgcagc ccgacaatct
     5101 gctactggcg accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt
     5161 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac
     5221 cctgctgtgg cacaaacgga acagccataa tccgaagatc gtctggttac gggaaaccat
     5281 taaaaatctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc ctatttcaca
     5341 ataaaatgta aaaaatttgt aataaacttg tctgaatcga acttttagcc gctttagtct
     5401 gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg
     5461 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     5521 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     5581 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     5641 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agcgataaaa atttgccttc
     5701 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     5761 tggcctcgat atggaactgc agcgtgcgac acgcgggttt catagcccac acgcgcccga
     5821 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     5881 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     5941 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     6001 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     6061 cacgcccacg attgaaaagc tcagtgtctg ggagttagat ggcaaacacg gctggaaaag
     6121 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     6181 gctctctacc atgaaggctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     6241 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     6301 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     6361 cattgccgac attcccctgt atgacgctga cgtacaggag gaagaaggtt ttccagcaac
     6421 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     6481 atataactat tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     6541 ggatcaaccg ctggccggta aaccggtgtt gattcagacc aactcaatgg gcgtgattgg
     6601 cggcgcgcgc tgtcagtatc acctgcgtca gattctggtt ttcctcgatg caatggtgat
     6661 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     6721 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     6781 tattcagcga gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt
     6841 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     6901 aacgatgatt acgcacgggc taaggtcacg gagacgcccg gtaccaatct tcatcacgca
     6961 gtaggagata aagcccagcg caataccttc ggtaatcgag aagctgaacg gcatcatcac
     7021 agcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     7081 tgaggtcatc aacacgccaa cgtaaatcag cgcgccagct gcagcgtagc ctggcaccat
     7141 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     7201 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     7261 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     7321 catgcgcggg aatttcccct tctcatctgc cagacctgct ttatcggtca cgccaatcaa
     7381 cgtaccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     7441 gaggttaaac gacccggcta aatctacatg acccacgaca gtcattacgc tcggcggcgc
     7501 agaaacgatg ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac
     7561 gatagaaacc agcaccgctg cgtgaatgtt gcgtgaggcc agaatagcaa tgatgaagaa
     7621 gccgaggatc cccagcagca cgctgtgaga agtcagatta ccgatgctga ccagcgtttc
     7681 cgggttagcg acaatcacac ctgcgttttt cagccccatc atgccaatga acagaccgat
     7741 accgctggta atgcccacac gcagactcac cggaatgttg gcaatcatcc agtagcgaac
     7801 gcggaaaatc gtcagtaata gcagaccaat cgcgccccag aagattgcgc ccatcccgac
     7861 ttgccacggc aagcccatcg cctgcacaac gacaaaagcg aagaacgcat tcaggcccat
     7921 agcgggtgcc agtgcaactg gcaggttagc aaacagcccc atcataatac tgccgaatgc
     7981 agcaatcaga caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     8041 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     8101 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     8161 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     8221 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca
     8281 ttgtatttgc ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa
     8341 ctaacgataa aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac
     8401 gctggtcgat agcgaggtca tttgctctcg cgcctatgtc acgatgttca gggaatttgg
     8461 cattaacgtt gacctggagg agatttttac ccgttttaaa ggcgtaaaac tctacgaaat
     8521 catcgacatc atcagtaaag aacatggcgt gacgatggtc caggccgatg ccgagcacgt
     8581 ttaccgcgca gaagtagcgc gtctgtttga taccgaactt gcggacatcg caggcgcgaa
     8641 cgaattgctg gcgagcgtta acgtgccgat gtgcgtcgtc tctaacggtc cggtcagcaa
     8701 aatgcagcat tcgctgggta agctcaacat gctgcactac ttcccggaaa aactgttcag
     8761 cggctacgat atccagcgtt ggaagcccga tccggcatta atgttccatg cggcaaaagc
     8821 gatgaatgtg aacgttgaaa actgcattct ggtcgatgac tccagcgctg gcgctcaatc
     8881 agggatagat gccggaatgg aagtgtttta cttctgcgct gacccgcaca ataagccgat
     8941 cgttcacccg aaagtcacca cctttaccga gctgtcgcag ctgcctgcgc tctggaaggc
     9001 acgcggctgg aatataacgc gataatcgcc tccttacttc aacgcttaca catcagtcac
     9061 gctggtttga gataaagaac accaggtgtc tgatgagtaa gcgtctattc cctatgaata
     9121 aattatgtcg ttattcgtcc tgtcatccac cctctgcata gttaagcctt ggttatattt
     9181 tttacaatat cggaatactg ttcgaaccaa tccagaatat tacgcatcgc gtcactgatg
     9241 tttttatcgt ctttatattg attctcaagt ctattatttc gaataatagt aagcttagaa
     9301 cgtattttat catcacaatc gacaattttc gcattcaatg catttacttc atcctgcact
     9361 tctatactat tccaattgtc ccttttgtta tcaaataaag tccttttatc ctctaatgat
     9421 ttattacgac ttatggttaa ctctttaatt tctcgcaata agtcatcgta atcttta
//
LOCUS       DAZGCQ010000159         9358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000159 DAZGCQ010000000
VERSION     DAZGCQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.159"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(231..1283)
                     /gene="fbaB"
                     /locus_tag="ACHK07_004614"
     CDS             complement(231..1283)
                     /gene="fbaB"
                     /locus_tag="ACHK07_004614"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO8104850.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1540..2817
                     /gene="yegT"
                     /locus_tag="ACHK07_004615"
     CDS             1540..2817
                     /gene="yegT"
                     /locus_tag="ACHK07_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO8104851.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2814..3818
                     /gene="yegU"
                     /locus_tag="ACHK07_004616"
     CDS             2814..3818
                     /gene="yegU"
                     /locus_tag="ACHK07_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO8104852.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRGEFTDDTSMALCLADALLEREGNIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            3815..4780
                     /gene="yegV"
                     /locus_tag="ACHK07_004617"
     CDS             3815..4780
                     /gene="yegV"
                     /locus_tag="ACHK07_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8104853.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4754..5500)
                     /gene="yegW"
                     /locus_tag="ACHK07_004618"
     CDS             complement(4754..5500)
                     /gene="yegW"
                     /locus_tag="ACHK07_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8104854.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5552..6370)
                     /gene="yegX"
                     /locus_tag="ACHK07_004619"
     CDS             complement(5552..6370)
                     /gene="yegX"
                     /locus_tag="ACHK07_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO8104855.1"
                     /translation="MQLRITSRKKLTALLCALVLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPGQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKLIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(6435..7235)
                     /gene="thiD"
                     /locus_tag="ACHK07_004620"
     CDS             complement(6435..7235)
                     /gene="thiD"
                     /locus_tag="ACHK07_004620"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO8104856.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7232..8020)
                     /gene="thiM"
                     /locus_tag="ACHK07_004621"
     CDS             complement(7232..8020)
                     /gene="thiM"
                     /locus_tag="ACHK07_004621"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO8104857.1"
                     /translation="MQVDLLSFAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8055..8151)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8759..9084
                     /locus_tag="ACHK07_004622"
                     /pseudo
     CDS             8759..9084
                     /locus_tag="ACHK07_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         2256 a         2369 c         2359 g         2374 t
ORIGIN      
        1 acgatcacaa aaacgacaat atgaaaatta atagaggaat gaaaggcaaa aaaaaacggc
       61 ctcccgtagg gaagccgcaa caaagtgcgc gtggttttat gcaggatgcg gcgtaaacat
      121 atgtggcagc gctgcgtagg catgataaga cgcttcagcg tcgcatcagg cataggttgc
      181 cggatgcggc gtaaacgcct tatccgtcct acggggtagt gccgttcgta tcaggcgata
      241 gtaattttgc tatcgagata aacgtcctgc acggcgttaa tcagtttcac gccgtcagcc
      301 atcgatttct tgaacgcctt acgtccaaga atcagcccca ttccgccagc gcgtttgttg
      361 ataaccgcag tacgcactgc atcactgagg tcagtttcac cgcctgcagc accgccggag
      421 tttatcaacc cggcccggcc catatagcag ttagctaact gataacgcac cagatcaatc
      481 gggttttcgc tggttaactt gctgtacacg cgatcgtcgg tataaccgta attaattgct
      541 ttatagccgc cgttattttc cgccattttt tgtttgacga tatctgcacc tatggttgcc
      601 gccagatggt tcgcctgacc agtcaggtcg gcggagacat ggtaatcaac gccatctttc
      661 ttaaaggcgg agttacgcaa ataggcccac agcactgtca ccatgcccag ctcgtgcgca
      721 cgttcaaaag ccgcagaaat ttcttcaatc tggcgacgtg actcttccga gccaaaatag
      781 atagtcgcac caaccgccac cgcacccatg ttgaacgcct gctccacgct ggcatacagc
      841 gtttgatcgt aggtattcgg gtaacttagc gtctcgttgt gattaagttt gacgagaaat
      901 ggaatgcgat gcgcgtaacg ccgcgatacc gacgccaaca cgccgtaagt tgacgccaca
      961 cagttacagc ccgcttcgat cgccagttca acaatgtttt tcgggtcaaa gtagagcggg
     1021 ttagcggcaa atgaagctcc agcagagtgc tcaacgccct ggtcaacggg cagaatagaa
     1081 agatatcctg tgccagccag acgcccggtg ttgtacaacg tctgcatatt acgtaacacc
     1141 gctggcgggc gattattgtc aatcatcacg cggtctacgt agtcatgtcc ggggagataa
     1201 agctggtcag aaggaatagt catacaacgg tgctgtaaaa ggttgtcggc gtctttgcca
     1261 agcaactgcg caatatctgt cattactatg ctcccgtaaa ttccgattgg atatcggcta
     1321 tggattgtcc tggcccgcct tttgcgggca atcataatcc tggtcgttac gggcgagttt
     1381 ttccatcttt tggatgtttt ttcagcgttt tctgttggct cgattcatca gaaaaaatgt
     1441 tagcgcggtc aagttttcac cgcaaaggta tttaaaaggt attattaagt ggtattgtca
     1501 tcgcgtacct tacattacct gtcatgaagg aattaaaaga tgaaaacaac agcaaagctg
     1561 tcgttcatga tgtttgttga atggtttatc tggggcgcgt ggtttgtgcc attgtggttg
     1621 tggttaagta aaagcggttt tagtgccgga gaaattggct ggtcgtatgc ctgcacagcc
     1681 attgcggcga tcctgtcgcc aattctggtt ggctccatca ctgaccgctt tttctcggcg
     1741 cagaaagtgc tggcggtatt gatgttcgct ggtgcggtgc tgatgtattt cgccgcgcaa
     1801 cagaccactt ttgccgggtt cttcccgtta ctgctggcct actcgctaac ctatatgccg
     1861 accattgcgc tgactaacag catcgctttt gccaacgtgc cggatgttga gcgtgatttc
     1921 ccgcgcattc gtgtgatggg cactatcggc tggattgcct ccggtctggc atgtggtttc
     1981 ttgccgcaaa tactggggta tgccgatatc tcaccgacta acatcccgct gctgattacc
     2041 gccggaagtt ctgctctgct cggtgtgttt gcgtttttcc tgcccgacac gccaccaaaa
     2101 agcaccggca aaatggacat taaagtcatg ctcggcctgg atgcgctgat cctgctgcgc
     2161 gataaaaact tcctcgtctt tttcttctgt tcattcctgt ttgcgatgcc actggcgttc
     2221 tattacatct ttgccaacgg ttatctgacc gaagttggca tgaaaaacgc caccggctgg
     2281 atgacgctcg gccagttctc tgaaatcttc tttatgctgg cattgccgtt tttcactaaa
     2341 cgctttggta tcaaaaaggt attattgctt ggtctgatca ccgctgcgat ccgctatggc
     2401 ttctttattt acggtagtgc ggatgaatat ttcacctacg cgttactgtt cctcggtatt
     2461 ttgcttcacg gcgtaagtta cgatttttac tacgttaccg cttacatcta tgtcgataaa
     2521 aaagcccccg tgcatatgcg taccgccgcg caggggctga tcacgctctg ctgccagggc
     2581 ttcggcagtt tgctcggcta tcgtcttggc ggtgtgatga tggaaaagat gttcgcttat
     2641 caggaaccgg taaacggact gactttcaac tggtccggga tgtggacttt tggcgcggtg
     2701 atgattgcca ttatcgccgt gctgttcatg atttttttcc gcgaatccga caacgaaatt
     2761 acggctatca aggtcgatga tcgcgatatt gcgttgacac aaggggaagt taaatgaaaa
     2821 cagaacgtat tctcggtgct ctttatgggc aggcgttagg ggatgcgatg gggatgccct
     2881 ccgagctttg gccacgcagc cgcgttaaag cacactttgg ctggattgac cgttttctgc
     2941 ctggaccaaa ggagaataac gcggcctgtt attttaaccg cggcgaattt accgatgaca
     3001 cctcgatggc gctgtgtctg gcggatgcgt tactggaacg tgaaggcaat atcgacccga
     3061 atctgattgg gcgtaatatt ctcgactggg cgctgcgttt cgacgccttt aacaaaaacg
     3121 tactaggtcc gacctcgaag attgctctca acgccattcg cgacggtaaa cccgttgctg
     3181 aactggaaaa caacggcgtg accaacggcg cagcgatgcg cgtctcgcca ttaggttgtt
     3241 tgcttccggc gcacgatgtt gattccttta ttgatgatgt ggcgctggcg tccagcccga
     3301 cccataaatc cgatctggca gttgcaggtg cggtagtcat cgcatgggcg atttctcgtg
     3361 ccattgacgg agaaagctgg tcagcgattg tagattcact gccttcaatt gcgcgacatg
     3421 cacagcaaaa acgcatcact accttcagcg cctcactggc agcacgtctg gagattgcgc
     3481 tgaaaattgt gcgcaatgcc gacggcactg aatccgccag cgaacagctt taccaggtcg
     3541 ttggcgcagg taccagcact attgagtccg ttccgtgcgc cattgcgctg gttgaactgg
     3601 cacaaaccga cccgaaccgt tgcgccgtcc tgtgcgctaa ccttggcggc gacacagaca
     3661 ccatcggtgc tatggcgacg gcaatctgcg gcgcgttgca tggcgttaac gctatcgatc
     3721 ctgcattaaa ggcggagctg gatgcggtaa atcagcttga tttcaaccgc tatgccacag
     3781 cgctggcgaa atatcgtcaa caacgggagg tggtatgagc ggcgctcgat tacacacgct
     3841 gctgcctgaa ttaaccacgc gtcagccggt gatggtcgtc ggcgcggcgg tcattgacgt
     3901 gatcgccgac gcctacgccc tcccctggcg tgggtgcgat atcgaactga aacagcagag
     3961 cgttaacgtt ggcggctgcg cactgaatat tgccgtggcg ttaaagcgcc tcggcatcga
     4021 agcgggtaat gccttgccgc tcggtcaggg cgtgtgggcg gagattattc gcaaccggat
     4081 ggcgaaagag gggttaatca gcctgatcga taacgccgaa ggcgataacg gctggtgtct
     4141 ggcgctggtt gagccggatg gcgaacgtac ttttatgtca ttcagcggtg tggaaaatca
     4201 gtggaatcgc cagtggctgg cgcgattaac cgttgcgcct ggcagcctgc tctatttttc
     4261 cggttatcaa ctggcctcgc cctgcggcga attgttagtg gaatggctgg aagagctgca
     4321 agacgtgacg ccgtttatcg attttggccc acgtattggc gatatcccgg atgcattact
     4381 ggcgcggatc atggcctgtc gccctttagt gtcgctcaat cgtcaagagg ctgagattgc
     4441 cgccgaacgt tttgctttat ccgcagagat aacaacactt ggcaaacaat ggcaggagaa
     4501 atttgccgcg ccgttgatcg ttcgtctcga taaagaaggc gcatggtatt tcagcaacga
     4561 cgcttctggc tgcattccgg catttccaac gcaagtcgtc gacaccattg gggcgggcga
     4621 cagtcatgcc ggtggcgtac ttgccgggct ggcctctggt ctgccactgg cggatgccgt
     4681 attactgggc aatgcagtgg cgtcgtgggt tgtcgggcat cggggcggtg attgtgcgcc
     4741 aacgcgcgag gaactactcc tcgcacacaa aaacgtatag atcgctgcga cagtggctaa
     4801 tgctgtactc aataggccgt tgctgttggt caagcgccac ttgcttgatc accagcaccg
     4861 gtattttgct atctaactga atatgtgact gaaactcggc atctggcatc cgggcgctaa
     4921 cgcgggaacg tgtacgttgt gggtaaatgt gctggctgcg gaaatagtca taaagagaaa
     4981 ttccgatggc atcaacatca tgaattaaat gcgccggaac ccacgattcc tcaatcgata
     5041 ccgcttcttc atcgacatag cgaatacgct taagcaaaaa gacatcactt cccgcttcga
     5101 ccgccagctg ctgcgcgact tcttcgggac atttcacgac acgcttgtta acccatagcg
     5161 tatcgggctt tttcccgcgc aataccacct gctgagaaaa acctcgcgct tctttcaacg
     5221 agtattcgaa gatgttgttg atctgcgtgc cgtaaccgcg cgaacgcgtc accacgccct
     5281 cttcttccag cgcctgcatc gccttgcgca ccgtaatgcg tgaaacgccg gttaactgac
     5341 ttaaatcacg ctcaccgggc aaaatattgc catgctccag caccccgctg cgcacggcat
     5401 tttttaccgt ttcggcaaac ttaatataaa gcggcgtgtt atccgccgct aaaatacgct
     5461 cgttaagttg ggcaatcagc tgggtatgcg cttgttccat ttgtcttttt cctgccaggg
     5521 agtctggtgg gagtatatca ctgcttctta tttaaggcgt ttctttaatc ccatcaacga
     5581 atgcctgtaa ctcctccacc gtgccattaa acacattaaa atccaccgga ccattgatgc
     5641 catctacctg tccacggtcg gaatgctgcc agaagcgcca ggccatgccg tcattgtccg
     5701 gacgacgttg atagtagtga gccacccacc acggatattc attgaaatag cccgccagat
     5761 tggtgtgata aaaaacggct cctgagtaaa taatcagctt ttttcccgta cttttttcga
     5821 ccatttttag ccactgactt acccgcttgc gtaattcttt tgccgataat tttccccgtt
     5881 cttctacgtc cagcacggca gggaaatcgc cttgtgagaa atccaccgtt tgcagaaata
     5941 atcgcgcctg accaggcgct gctaccgacg gggagaaata atgatacgcc ccgcgcagca
     6001 ggccattttc gcggcttagt tgccagttac gcgaaaaata ggggtccacc agcttttcgc
     6061 cttccgtagc cttaataaaa gcaaactgta agcggatacc gttgtcgcgc atttttgcca
     6121 cccgctgcca gtcgatccgc tcctgccagc gcgaaacatc tattccgtga atggtgtaac
     6181 tggcaggaat gcgaatagcg aaagatttaa ccgggcgata accgtagaag tggatgtagt
     6241 ctgtaatctg aactgctgtc gaatagaaaa aattcaccgt ctgacgcgga taaattgcaa
     6301 cgatggaaat tagcacaaga gcgcataata gagcggtgag tttttttcga ctggtgattc
     6361 tcagctgcat tggtttcatc cctgaatgtc agtgccaaag gctgacaata accaaagcag
     6421 ctatagtacg gtgctcacca ccaggcgtgg aagtggtgaa ccggaccaat accgtgacca
     6481 acttccagcg tgtcggcctg ggctaatgct gatgaaagcc agctttttgc ctcctgtacg
     6541 gtgtcagccc agtttgtatg gcgcgggcgt agtgcagcca acgccgcaga gagcgtacaa
     6601 ccagtgccgt gggtgttttt ggtcataatg cgcggtgcgg taaaccgttg ttcaccctcg
     6661 cgggtaaaca gccagtccgg gctttgctca tcatccagat gaccaccttt cattagcact
     6721 gcgccacagc ccatcgccaa cagcgatcgc ccttgttcca gcatttcctg ttcggtgcgc
     6781 gcgtgtggcg cgtcgagcaa ggcagcagct tcgggcaagt ttggcgttat taatgaaacc
     6841 tgtggcaata atcgactgcg caacgtagca accgccgaag gtgaaagcag cgggtcgccg
     6901 ctttttgcca gcataacggt gtcgagtacc acgttttgga tctgataacg ttgcaaccgt
     6961 tctgccaccg cttcaacaat atcggtttcc gccaacatac cgattttagt ggtatcgatt
     7021 cgcacatcgc tgaacaccga atcgagctgg gcggcgacaa aatcaggctc aatgcgatac
     7081 accgactgta cgccacgggt attttgcgcc accagtgcag taataactga gcaaccataa
     7141 gcgccaagtg ccgagaaggt tttaagatcg gcctgaatcc ccgcaccacc actcggatca
     7201 gtaccggcaa tcgtcagagc gttaattcgt ttcatgcctg cacctcctgc gtcaattgcc
     7261 agagcgcatc aaggaaatgt ggaacaaaac tgcctggccc ctcgcttctg gcgactgcgc
     7321 gttctccggc ttgtttcatc cagtgacagg cagatgcgac attttccagc gtatcgcctg
     7381 gtaacgcaca gcaggcagcg acaaccgccg ataacgcaca gccagttcct accactttgg
     7441 tcattaacgg atcgccaccg tgaataccaa tgatacgatg tccatcggta acataatcca
     7501 cctcgccagt gaccacgacg attgcgccag tttcccgtgc cagtgtttgt gcagcgggta
     7561 tcgcattagc tgccgcgtca gtggtatcca ctccccgtcc gccagtagca acgccagcta
     7621 atgccatgat ttccgaagca ttaccacgta tcgctgccgg tttaaaagat aaaagttcat
     7681 gacaaaaatg gcgacgataa tcgagcgcac caaccgctac tggatcaagc gtccaggggg
     7741 tttgagagct ttttgcttgc tcaacggcgg cacgcatcgc ctgtgcgcgt ggctgcgtca
     7801 gcgtgccaac gttaatcaac aaggcactgg cgatagccgc aaactgactg gcctcttcgg
     7861 tttcgataac catcgctggc gatgcaccga tcgccagcaa ggtattggca gtaaaggttt
     7921 gcaccacatc gttggtcatg cagtgcacaa gaggggaatg ttggtgaaaa aggtgtaacg
     7981 tgtgcgcaga ttgcgcgaaa ctcagcaggt cgacttgcat agtttgctcc tgccataacg
     8041 tgaagaagca atgacctggt ggtccgtgac ttccctacgc tggcattatc cagatcaggt
     8101 gatacgggta tttctcagcc ttcacgcaga agggcacccc gagtcgtttg gttgaatctc
     8161 tatattttgt gctagattag gggaagtggc ttgcagatgc aaatttaccc tcaagtcatt
     8221 ttcccggctt acgtggtagc tggcgtagcc agcagagaat cgggtcatat aaatatgacc
     8281 agcctgtatc atacaacctt ttgtaagaag gcttcagtat tatcacgccg gaccaagtat
     8341 ggatagaaaa gatcacatac ctttatacca taaatgcagt ttgtatctga caatagtgat
     8401 taacctgtag tgatattcca ttattggttg gtcatttttt gcagattact gcatcattag
     8461 tctgtaacag actacagata gagaggttgg agcgtaagct tctataaaac gttgttaagt
     8521 acttcgtata atcgtgcaat tcgcatttag agatcctcag gcataatcaa tctgccaatg
     8581 aaggggagcg ccatgcataa agcccgtatt actgaacatc acgccgtgat tatatcgatt
     8641 gaatccggac aaaccgttaa agatgtctcc cgggatgccg gtatctctga agccatctac
     8701 tacaactgaa agtccagatt aggcggcatg agggcttctt atattaaaaa gaaaatgact
     8761 cttgatgcac atgggtaatg gtccggaatg tatctttctg aggctgtctg aacgggactg
     8821 agaaacatgg tttaatactg gagtttatct tgccggataa accgacaaag aacgctttca
     8881 tggagcgttt taataagaca tacttgtaca taaatactcg atttttatct attcagaacg
     8941 ctgaaagaaa tgttggaaat cacggaataa tgtttatcag aatataacag ataataccac
     9001 atgaatcgca gaacaatatt tctgccggag gaataccgat ggcgccatta ttttgtatgg
     9061 ttctcaaaaa atgcatggaa ctaaaaccgt ctattttctt ctcgttctag ctcaaaattt
     9121 ttgaagatca tgaaaacaat caaatcggcg aatagatatg gttaacctaa tttttacata
     9181 tatataagaa tgagataatt tttctacgat ggaatgcgct ccctcttaaa aggtatatct
     9241 atagaagcct catcgcctaa aaaaacaaaa taaaaacatt tttgatacaa ttgaaacaac
     9301 aaaacaaaac acaattcaaa cgtaattcac ataaccatgt gaaacatggt cttagccg
//
LOCUS       DAZGCQ010000160         9324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000160 DAZGCQ010000000
VERSION     DAZGCQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.160"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1720
                     /gene="adeD"
                     /locus_tag="ACHK07_004623"
     CDS             <1..1720
                     /gene="adeD"
                     /locus_tag="ACHK07_004623"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO8104858.1"
                     /translation="YQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIA
                     GVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICD
                     PHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRD
                     HPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENC
                     HESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIA
                     HEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDAR
                     KVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRY
                     RVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGA
                     LAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMST
                     DTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFT
                     TLEVTE"
     gene            complement(1768..3159)
                     /gene="uhpT"
                     /locus_tag="ACHK07_004624"
     CDS             complement(1768..3159)
                     /gene="uhpT"
                     /locus_tag="ACHK07_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO8104859.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3297..4616)
                     /gene="uhpC"
                     /locus_tag="ACHK07_004625"
     CDS             complement(3297..4616)
                     /gene="uhpC"
                     /locus_tag="ACHK07_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8104860.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4626..6128)
                     /gene="uhpB"
                     /locus_tag="ACHK07_004626"
     CDS             complement(4626..6128)
                     /gene="uhpB"
                     /locus_tag="ACHK07_004626"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO8104861.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6128..6718)
                     /gene="uhpA"
                     /locus_tag="ACHK07_004627"
     CDS             complement(6128..6718)
                     /gene="uhpA"
                     /locus_tag="ACHK07_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO8104862.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6880..7983)
                     /locus_tag="ACHK07_004628"
     CDS             complement(6880..7983)
                     /locus_tag="ACHK07_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104863.1"
                     /translation="MNSYIKIEQTGSNKFKVTDMYREFNDETKCFDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDRQTERRMIELIRE
                     QKDLIPEKYLHQSSIKNLKLHEDEFSSLLADTERQVLEGSSFVLCCGEKINGTISELL
                     RKKITDSTHSIKSLTLSESPSYEVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIGNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSLVALNGRSY
                     HQHVDFSEYGFTQTLCMLGAVVNNANDTFDVANYIYTTKGENIIDNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDVRNLRRNFSGNITIATYK"
     gene            complement(8591..>9324)
                     /locus_tag="ACHK07_004629"
     CDS             complement(8591..>9324)
                     /locus_tag="ACHK07_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104864.1"
                     /translation="LRVQQDEGNLPVQKDPEFPDDCFDSMRADAHRCVKDQEQKKSGT
                     KINSGTKITCCGVELSKDILKILADKTKKYETAVEHTLGLVVADVCFKEDECSQVREL
                     LKKPNGNVTDDKIDEIVACLASPQGITCFYYSSIQFYQRYLKEKSSDVQELLKNSSES
                     FSSPSGLDNQGNIFVGHAKYVNEMDFTINSFSQKMYVAMVKNVDNEVKLQCARYITTG
                     SVDEQNSISDKIAFTFYGAATTADL"
BASE COUNT         2477 a         2467 c         2177 g         2203 t
ORIGIN      
        1 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
       61 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      121 acgttacatt gccggtgttg gcgcagaata cactgatgct ccggctttgc agcggattga
      181 tgcccgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      241 catgatgacg ccggtcacct ttgaaaccgc tactctgccg cgcggcctga cgaccgttat
      301 ttgcgaccct catgaaatcg tcaacgtgat gggcgaagcc ggattcgcct ggtttgcccg
      361 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tgcccgctct
      421 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      481 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      541 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      601 gggtttgggt ggtaaagaac ttaacgccta tattgctgcg ggtattgaaa actgccacga
      661 aagttatcag ctggaagaag gacggcggaa attacaactc ggcatgtcgt tgatgatccg
      721 cgaagggtcc gctgcccgca atctcaacgc actggcaccg ttgatcaacg aatttaacag
      781 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gaaatcgctc atgaagggca
      841 tatcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
      901 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
      961 acccggaaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1021 ggtactggtg aaaggcgaac cgattgacgc gcaaacctta caggcggaag agtcggcgag
     1081 actggcacaa tccgctccgc cgtatggcaa taccattgcc cgccagccag tttccgccag
     1141 cgactttgcc ctgcaattca cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1201 cgaattgatt acgcactccc gctccagcgt ctacagcgaa aatggttttg atcgcgatga
     1261 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgt ctggctccgg cctgtggttt
     1321 gctcggcggc tttggcctga atgaaggcgc gctggctgcg acggtcagcc atgacagcca
     1381 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1441 tcaggatggc ggcgggttgt gcgtggtacg taacggccag gtacaaagtc atctaccgtt
     1501 gcccattgcc gggctgatga gcaccgacac ggcgcagtcg ctggccgagc aaattgacgc
     1561 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1621 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgg
     1681 cgagaagttt gccttcacca cgctggaagt cacggaataa taaaaaaaaa gcccggcgtc
     1741 atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc
     1801 gcggcggatt ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga tacaaccaat
     1861 cgcggcgata tcaagtgcgg cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt
     1921 cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa
     1981 ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagcagc
     2041 cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag
     2101 atagatatat tcgttgctgg catgttgata cacaccgagc gtggcgataa tcagcgccag
     2161 cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag
     2221 cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt
     2281 agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac caatacgtac
     2341 cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata
     2401 ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt cctcttcgct
     2461 gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg gggagtcgct
     2521 gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga agataaacat
     2581 gccgatgaca tggccatcga acaggtaatt tgcgccgaac agcgccacac ctgctgcgcc
     2641 tgcaccgcca aggttgtgag atatgttcca gaaaccgagg aaggtcccgc gcttacgacg
     2701 cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtgctct ggaaaaagcc
     2761 gcttaaggcg tagaaggcaa tcatcagaaa caggctaacc gagccgctgc ccatactggc
     2821 actgaagccc agcatacaaa tggcagacag gataagcatg aacggcagga attgtttggt
     2881 gtttttgccg tcggcgtagt aggaaaccag cgttttaccc acgccataag tgatggagaa
     2941 acccaggccg atcatcccca actgcgtcat gctcaacccg taggtcgaaa tcatatcgtt
     3001 ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga taaagaccac
     3061 caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc
     3121 cagggtcggc ttgcgaacct ggtttaagaa agccagcatg ggttactcct gaaatgaata
     3181 cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat agcgtccagg
     3241 caaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga gatgcatcac
     3301 gcttcgcgcg gtgcctgggc gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg
     3361 gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagtac tttcgccagt
     3421 ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc
     3481 cctgccgcct ctttgtggga acactctgcc gccgccatgc cgattaacat ctgcgggcca
     3541 aagacaaaaa aaccaatagt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc
     3601 atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc
     3661 ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc accgataaat
     3721 ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac gcccagcgtc
     3781 tcggacatat acaaattgcc ccagtcgttg attgccgccc ggaccacata gaccagcaca
     3841 tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catacttggt gaggatctct
     3901 ttacgcgtca gccctgcccc ttcttgttgt tgagcaatct ccagcgcgtc gtgccgccat
     3961 tcacctaccg ccggtaaacc taacgcctgc gggcgatcgc gcagccgcca gcagagaaaa
     4021 atccccacga cgatcgccat acaaccagca atcatcatcc cggcacgcca gccgtaatgt
     4081 agtgcagccg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc
     4141 cataatgccc accaaccgcc gcgctcggta cgagaatacc aggcggttaa cagacgcgca
     4201 cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagtac ggcaaacgcc
     4261 catagcgacg tcgagaagcc aaacagaatg ttgataatgc ccgtggcgat aagccctatc
     4321 cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg
     4381 ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct gagcacgccg
     4441 ttagcaagga tttctggtac ggcggcgtta aaacttttcc gcgtgaagta aaacagcgcg
     4501 taacccagcc agatggtcag cagaatatgc cgacgccagt agcgatagcg tgcatcaatt
     4561 tcatgtttgt cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc
     4621 gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg cagacaggaa
     4681 atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag gccaaaacct
     4741 tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag catcaaccgt
     4801 tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt cacaatgttg
     4861 ttcagccctt cctggcagac gcgaaacagc gtcacgcgct ggttttcact taacgctgac
     4921 tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc catttcccgc
     4981 atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg acgtaaccgc
     5041 cctaacaaac ggcgcaccgc atcgtaaaca cccagcgaca gttgttcgat gagctgcccg
     5101 ctctgcttca cgctggcgtt atctgccgcc agtcgctgga caatgcccgc ctgagtacga
     5161 atggcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacatc acggcgcacg
     5221 ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag ttccttttgt
     5281 agcgactggt taagttcacg caaccgctgg atgcctgcgc ctagtaacaa ccctgtcaga
     5341 ctttgcacca gcaacgagag caataaatcc accggatgat cgcgccaggt ttgactggcg
     5401 atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccaacc atagtgccag
     5461 gccagcgcaa taatcggcag cgccaggcag aatggcgtaa agcgcgacag ttcgtccggc
     5521 aatcccaact ggagccagag actaataaca aacagcagca agtaccagac cagatgccgc
     5581 ccgcgccagt tgatcggttg agaaactaac gacgggccga gcggcagcca ggtgttattg
     5641 gcgagatagt gccagaacac cagacatatc ggggccagcg tcaggccgcc agttaaagtc
     5701 agtagtagcg cattccacga ctctttgccg tgccaaagcc agggcagcga ctgcaacaac
     5761 gccgccgccg ttaacgccgc cccctgtaac agcaaggtgc gccagtcacg ctgatggcga
     5821 tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac taccgatcat caataaggaa
     5881 aaatgggtta acccgaccgc ctgcgttagc cagtaaatca gcagccattc cgcgcccagc
     5941 aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag accaaacgga
     6001 aataacagca ccgccatatc agggcgctca accaggtgca ggctgatact ccacaggcaa
     6061 aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg ggagaacaac
     6121 gtcttcatca ccagccatca aacatgcggc gcgccagttc aacgtcgtta ctgacgccca
     6181 gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag cccagttcgg
     6241 cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg cgttcgcgtt
     6301 tggtcagcgg gtcctggcga ccggatgcca gtttaatggc aatatccggc gtcagataac
     6361 agccgcccgt ggcaaccgta tgcaccgccg caatcagttc gtcagggcta cagcgtttgg
     6421 agagaaagcc gcgcgccccc gcgttaagcg cctgctcaac cagcgccgga ctgtcatgaa
     6481 cggagagcat aattgtcgcc atacctttcg gcagctggct tagcagttcc agaccggaga
     6541 tatcgggcat ggagatatcg caaatacaca cctgcacacc ccgccccggc agccccgcca
     6601 gcgcctcgcg ccccgaacca aactcggcaa ctacttgcaa atcaggctcc agccccagca
     6661 actgcgcaaa gccggagcgg acgataaggt gatcgtctat aagggcaacg gtgatcatga
     6721 ttgggtcctg gcggataaaa aacgcgcata ccttaacgat aagcgcgatg ttgttcaagt
     6781 tggatatggg gtatgtattg gctgaatgat gttgggctta tcgtcaaaac gcttacctga
     6841 aaggtaagcg tttggtgcgt gttattgaga aaaagcgaat tatttgtagg ttgcaatcgt
     6901 aatattgcct gagaaatttc tccttagatt gcggacatca ttatcccttg cccaataatc
     6961 atctttgtgt ctataacttt catcggaatg tacataggga gaatcatctt cagcgtcgtt
     7021 atcgataata ttttcgccct tagtggtata gatataatta gcaacatcaa aggtgtcatt
     7081 cgcgttattt acgactgctc ctaacatgca aagggtttgt gtgaatccgt attctgagaa
     7141 atcgacatgc tgatggtaac ttctaccatt caaagcaacc aaactcggac aaacatctat
     7201 tgtatcgcca acagatgtag cggcgcgacg cacatcaagt gcagcgttat cattagcaac
     7261 atgaaatgct tgtgaacagg cataaaacaa atttccaatc ccggtacgac tggataaaca
     7321 agaaataatt tttgctattt tttctggctt tataccatcc tgcactaaag aatctctcac
     7381 cagatgacat tcatcaggtg tgaaaacagc tttcttgaat atttcctcat aaacttcata
     7441 agaaggagat tcagagagag tgagagattt gatggaatgt gttgaatctg taattttttt
     7501 gcgaagtaat tcacttatag tgccgtttat tttttcacca caacataaaa caaatgaact
     7561 tccctccaga acttgtcttt ctgtatctgc aaggagtgag ctaaattcat cctcatgaag
     7621 cttaaggttt tttatagatg attgatgtaa atacttttct ggaattaaat ctttttgttc
     7681 ccttataagc tctatcattc ggcgttcggt ttgcctatca aaagagtatt tcatgttata
     7741 gttattttta gcaacttcca gcttaagagc atttgcagca actgttaatt ctgagatttg
     7801 atctttttca aattgatcgt gatctgataa taaccttata aactctttta cgtttcgacc
     7861 actaaaatcc atatccctac actttttatc aaaacacttt gtttcgtcat taaactcacg
     7921 atacatatca gtgactttaa atttattact tccggtctgc tcaattttta tatagctatt
     7981 catgcctgta acggcaatcc atgtcttata actcgcattc tccgattcag gcgtaacatg
     8041 aaaataagga accaatttat cactaatatc atgccgatag atatgaaatg aatctaaaag
     8101 atataataaa aaacttcgaa aaatgtcacg aaaagtgtca tctttaaacc ttgccctata
     8161 attatcgagc aggccgcttt gttctatcat attaaattta tgaagattat cctcgagact
     8221 tttgtcgata ctctccctta gactctgact ctgaaactgg ctattatcat tctctgtttc
     8281 cgttggatat gtataactcg tcacactaac catagtaacc tcgcaatatg acactttgca
     8341 tattttgaca ttgttagaag catcaatggt tagcatgata aatatctcaa tactaaccgc
     8401 tatatttcat ttatttcaat aataagacta tagtgcagtt aatttttatc gatttcccca
     8461 atgcaattgc atcactaaca ctacttattc actatcaaaa atcgctcatt tttttaaaaa
     8521 gactgtttac acacagacgg ttaagaatac gcgatcaact taatgataaa cgcgatttat
     8581 caggcaaaaa tcataaatca gcagttgttg cggcaccgta aaatgtaaaa gcgattttat
     8641 cgctgatgga gttttgttcg tcgacactac cggtagtaat ataacgagcg cactgaagct
     8701 taacttcatt atcaacgttt ttcaccatcg ctacatacat cttttggcta aaactattta
     8761 tagtaaaatc catttcatta acatatttag catgacccac aaaaatattt ccctggttgt
     8821 ccaaaccact gggtgaacta aaactttcgg aactattttt cagcaactct tgaacatcag
     8881 aagatttttc ttttaaatat ctttgataga attgtatact agaataataa aaacatgtaa
     8941 tcccttgagg actggctaag caagcgacta tttcatcaat cttatcatcc gtaacgttac
     9001 cgtttggttt ttttaataac tctcttacct gagaacactc atcttcttta aagcatacgt
     9061 ctgcaaccac gagtccaagg gtatgttcaa cagcggtctc atatttttta gttttatctg
     9121 ctaatatttt aagaatatct ttacttaact caacaccgca acaagtaatt ttggtgccgc
     9181 tatttatttt tgtgccagat tttttttgtt cttgatcttt tacacatcta tgagcatctg
     9241 cccgcataga atcgaagcaa tcatcaggaa attcaggatc tttctgaaca ggtaaattcc
     9301 cctcgtcctg ctgaactctc aact
//
LOCUS       DAZGCQ010000161         9318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000161 DAZGCQ010000000
VERSION     DAZGCQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.161"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            623..2179
                     /locus_tag="ACHK07_004630"
     CDS             623..2179
                     /locus_tag="ACHK07_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8104865.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGWIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2229..2948
                     /locus_tag="ACHK07_004631"
     CDS             2229..2948
                     /locus_tag="ACHK07_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8104866.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2959..4374
                     /locus_tag="ACHK07_004632"
     CDS             2959..4374
                     /locus_tag="ACHK07_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8104867.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4379..5074
                     /locus_tag="ACHK07_004633"
     CDS             4379..5074
                     /locus_tag="ACHK07_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8104868.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(5254..8370)
                     /locus_tag="ACHK07_004634"
     CDS             complement(5254..8370)
                     /locus_tag="ACHK07_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO8104869.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPIFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESENEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGHL"
     gene            complement(8490..9318)
                     /locus_tag="ACHK07_004635"
                     /pseudo
     CDS             complement(8490..9318)
                     /locus_tag="ACHK07_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293053.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2406 a         2060 c         2174 g         2678 t
ORIGIN      
        1 gtgggagaat tcgctctccg gggcgtctgc gtccccggcg gagaataaac cggagatgat
       61 gattctccac aggagtattc aatggcatac cattccccgg atgtcacact gcattatccg
      121 tatctgcgcc ggatgtcctg acacgtctgc ctgaatggcc ggaagagcgc ttgcacgact
      181 tactaccgtt ctcagcctac cgattcgcag aattaactga cgttacggtc ataccaccta
      241 atgcagggaa acgagtcatg gaggtcgtca gctctactcc catgaatgac acagcctctg
      301 aagtaaaaat accgacgcag tgaaaccagc cttaagcttt ccaaaacaca attacaacat
      361 agttgaacaa agttcattca aacaggttct tttaataaca tttagtcaaa taaaataaca
      421 ataaaacgat tcagcaatca tttttcataa caaaaacaaa ttataacccc cttccctgtt
      481 tgtggacgag actcgttctc tccctgcgag acaagcgtaa attggtcaca ccgacggtag
      541 ttgcctgaaa attacaaatt ctcgcctgac agggcaataa caacagtaat gatgctgaat
      601 ttgttttcgt tgaggaaatg acatgcacca atacctgctt tttttactgg gacttacccc
      661 acttctggca atgatcattt tgattctgaa agtcaaaatg cccatacatt atgccgtact
      721 cagcacactc tttattacac tgattcttgc aactttgttc tggcacacac cagttcagga
      781 tctgggttct gctgttgtat atggcgcaat caaagggctg tggccgatag tgatagtcat
      841 acttgctgcc atttacagct ataacctgat gaatgcgaca aaaagcatga acattctgcg
      901 aaatgttctg gccgctgtca gtgatgataa acgtatccag gtgcttctta tctcatggtg
      961 ttttggtgga tttcttgagg cggcggccgg gtacggtaca gctgtagcaa tccccattgg
     1021 tatactcatt gcactgggct ttaaccctct taaagccgca attgcatcat tggttgctaa
     1081 tacagtacct accgcgtttg gtgctgtcgg catacctgta tcagtacttg ctgaacaggt
     1141 aaatttaccc gtaacaaccc tcagcgggac gattattctt cagctggcag cgttcaacat
     1201 tctgcttccc ttcgttatag tcgctattat tggggaaggc ctgaaggcta tacgaggcgt
     1261 tgggttcatg accttaatgt gcggtgtgag cacattaatc ccacagtacc tggtggctgt
     1321 ttatcttggc gcagaacttc ctgctttcgc gggaagtctg gtcagcctga tcgtgatagt
     1381 ctttatggca aaaaaccgta aagaaaaaac agctcctgaa tttcttattg agacttcagc
     1441 agatagcgcc agcgcacttt catactctcg tggggcgttg ttacgtgcgt gctcaatcta
     1501 catcctgatt tttctgttca ttcttctttg ctctccactg ttcccgacgg taaaaagtac
     1561 agtgtctcag attgcctcgt cactcagctt ctctctgagt aacggcagca atctgtcgat
     1621 gaagattgaa tggatcgcaa cccccggtgt tctgattatc attgccagtg ttattggcgg
     1681 ctggatacag aaagcatccc tgtcatgcct gttaaatgta tttgtcagta cggtaaaaca
     1741 gctcaaaaac tccattattg ccatttctgc gattgttgct atggcaacgg taatggatgt
     1801 ttgtgggttg attggcactc tggcacagag ccttgtagat ctcactggtg gggcctacct
     1861 cttcattgct ccggttatcg gcgctcttgg tacatttgtt actggcagtg acacaaactc
     1921 caacattctg tttggtaaat tgcagaccac tgcggctgaa aaattgcatg tggacccact
     1981 ctggctggct gccgctaaca catctggcgc aacaggcggc aaaatgattt ctcctcagag
     2041 tattgctatc gctgtttcag ccacccgtat ggatggtcag gggagcgtaa tcatgtcagg
     2101 gacactgaag tactgctttg cttatattgt tattcttgga ctgaaagtag gccttgtcta
     2161 ccacctgttc atggcctgag aaaaaataac aaacggacgt ggaatattaa tcaacaggag
     2221 aaatatctgt gaatgttaac tttttcgtca cctgcattgg tgatgcattt aaatcccgta
     2281 tggcacaaaa cagcgtgctg ttactggagc aattgggttg caatgtccat ttccccgaaa
     2341 aacagggatg ttgcggtcag ccagcgataa atggcggtta tgttcgtgat gccattcctg
     2401 gtatgaaaaa tctcatatct tcgctggaag agaatgactg gccggtagta tctcctgccg
     2461 ggtcatgcac ttacacgata aaaaattacg ctcgctatct cagtgaagaa cctgagtggg
     2521 cggcacgagc tgaaaaagta gctgaacgcc tgcatgattt gacgtcattt atagtaaatc
     2581 atcttggcat cacagatgtt ggggcaagac tcaatggtcg cgcagtgtac catccttcat
     2641 gcagcctttc ccgtaaactg ggtgtaaccc gagaaccgct tttgttgctt gagaatgtac
     2701 ggggacttga gctgctgccg tttcacgctc aggaaacgtg ctgcgggttt ggcggcacgt
     2761 tctctgtaaa aatggcggag atctctggtg aaatggtgaa agagaaagtc gggcatatta
     2821 tggatgttaa acctgatttt cttatcgggg ctgacgccag ttgcctgctc aacatcggcg
     2881 gtcggcttag ccgtgaaggg catcctgtaa aagtgatgca tattgctgaa gtcctgatgc
     2941 acagatgagg taaacagaat gtctttgaaa acaagtgata tcaggtttaa agagcgtatc
     3001 cgtattcaga ttcaggaccc gatcatgcga aaagctgtgg ctaacgcaca ggagcgcata
     3061 gggactaatc gacagaaaat ggttgatgaa ctggggcact gggaagaatg gcgttcactt
     3121 gctgaacaga ttcgtcggca tgttctggaa aatcttgatg cttatttgta tcaattgtcc
     3181 gaaaaagtta ctgcgaatgg cggacatgta tattttgccc gtaccagaga ggaggcaacc
     3241 agttacatac tgaaggtggc gaaagaaaaa aacgccagga aggttgttaa atccaaatca
     3301 atggtgactg aggaaattgg tatgaacggc gttcttcaga aagccgggat accagtgata
     3361 gaaacagatc ttggtgaata tattcttcag ctggcgcatg agccgccatc gcatgttgtt
     3421 gtgccggcaa tacataaaga tcgccatcaa attcgcagag ttttgcatga acatctgggg
     3481 tatgacggcc ctgaaactcc agaagcaatg acattgttca tccgtgagaa aatcagacag
     3541 gattttctga gtgctgaagt tggcgtaaca gggtgtaact ttgccgttgc agaaacaggc
     3601 tcagtttgcc ttgtgacaaa cgaaggtaac gcacgtatgt gcaccacgtt acctaaaaca
     3661 catattgctg tgatgggtat ggagcgtatt gcaccaacat tccgggaggt ggatgttctt
     3721 atcaccatgc ttgcaaggag tgctgtggga gcaagactga caggttataa cacctggctt
     3781 acaggtccaa gagaagaagg acatgttgac ggcccggaag agttccatct cgtcattgtt
     3841 gacaatgggc gttcagaggt tctcggctca gaattcagcg atgtattgcg ctgtattcgc
     3901 tgcggtgcct gtattaatac ctgtcccgct tatcgccaga ttggtggaca tggctatggc
     3961 tcaatctacc cgggcccaat cggagcagtt atttccccgt tgcttggtgg atatgaagac
     4021 tttaaggaat taccttatgc ctgctcattg tgcactgctt gcaatggggt atgtccggta
     4081 aaaatacctc tggcacaact aatacaaaaa catcggcagg ttatggcaga gtccgaaata
     4141 acaccatcaa cagaacgccg tataaccaga atattcaatt acgtaaacag tcatccggca
     4201 ttgtggaaag ttggcatgat tgctggcgcg aaaacagcca gcctgtgcat caggaacgga
     4261 aaaacgccga ttagcccagg tgctattaaa gaatggtctg tggccagaga cttaccttct
     4321 cctgatggag aaagcttccg tagttggttc aaaagacata aaaaaggagg gtaactttat
     4381 ggaaaacaga gaggaatttc tcgttgctat cgccagaaag cttggcaggc cagtaagacg
     4441 tattcccgaa gcaatgccgg agcctgtaag tacgctggct acgacacgtt taaccgagtt
     4501 gacaatggac caacgctgtg aggcttttat taaatttgcc tcagaagtga tgcttgcgaa
     4561 gtgcgttctg gtttcgcctg aagacgcacc atcaaaagca ttagacatct gttggacgtt
     4621 cggttccggg ccagtcatta tcagcaacga tcaacgactg tttgatacgg ggattgcacc
     4681 attgctacaa aaagagatgg gcgctgtcct gtggaccccg gagaagggtg aggaaaatat
     4741 tcatcttgca gaaaaagcaa aaacaggcgt cgttttcgct gagtatggac tgacagaatc
     4801 tggtggtgtt gttttattct ccgcccctga acgagggcgt tcagtaagtc ttttacctga
     4861 agcgtctgtg tttgttttac gcaaaagtac cattcttccc agagtggctc agcttgcgca
     4921 aaaacttcat gatatggctc aggaaggaca acgtatgcct tcctgtatca atctgattgg
     4981 aggcccaagt tctactgcag atatagaact gatcaaagtt gttggtgtgc atggacctgt
     5041 acatgcaact tacttgatta ttgatgactg ctaatacatt ttagtatgta tatatgatta
     5101 acagctccgt ttagctgagc tgttaatcat tcatttagtt gtattagtcg tgacttgagc
     5161 gtaagcgtca atacggactc cgttaacgcc ttacaatagc agtcagtgtt catgcctgtt
     5221 tattgaggca agggggagtc tgggcgcctg taactaaaga tgccccccca cgcctttaaa
     5281 cttctcaata aatgcgctaa tcttctgaaa aacggtctgt tttttcgttt tatattgcgg
     5341 gtttaacgga ctaagttttg gtaatgtctc gtttaattct gtgccatttt cggtggcgta
     5401 ttcgcgtttt aacgacgtgc gaatatagcg cctggccgct tcttcattaa gattttcttc
     5461 tttgatcaat gcatctgctt cacgttgttg ttcgcgttga gcaaaggtaa agaacgtatc
     5521 aatgatgctg gctttgtccg gcaaatcatc aaggttcgtt tgctgaataa aatcgaccac
     5581 caggccttct ttagcacggt tccccagact tgaacgaatt aatcgtttga cctctacggt
     5641 catttcgtcc ttacttttgt tttgtctgct gtgttcgaaa atcaggccaa ggatataatc
     5701 caggtttatt tcctgagact tcagcaaatc gacctcaaaa accacgtcat cccagtcagt
     5761 ggttgatttt tcttttttgt cagcttcttt ttcgcggtgc tgccagtcac gaatatcgtt
     5821 ataggcagaa cgataatcct gaatcttgcg ttcagcaggg agacgaatca tttgcaattc
     5881 agcgaacttt tcatcatcca cataatgttc tgctttgaat ttttctaccg caacaggatc
     5941 gctgagatcg atttgttgca gggctttcag cgtggcaaat tcatcatagt tttgcaggac
     6001 gttctcggca cgcaggtatt caccaaacag tttaacgaag tcttttttct cgttttcgct
     6061 ttcaatactg gcagggtcag ggaacctttg ttccagttct gaaactactg ccataaagcc
     6121 gcgtttagct tcgccagtgg cagcatctgt aaagccttcc atatactctg cataactctt
     6181 ttccagcacc acatttctgg tgtttttatc accaaaaagt gtgatggcat cgatggttgc
     6241 ctgctccagg ttacggaatg tgacgatgtt accgaaggtt ttcgtggcgt cataaatgcg
     6301 gttggtacgg gaaaacgcct gcatcagacc gtgaaaacgc aggtttttat cgacgaacag
     6361 cgtgttcagt gttggagcat cgaagccggt taaaaacatc cccacgacga ttaacaggtc
     6421 aacatcctga tttttaaccc gctgagccag gtcccgatag tagttctgaa aatccttgct
     6481 atcagtactg aaattcattt gaaaatggtc gttatattca cgaattgcag catccagaaa
     6541 ctctttagca ctgctgtcca ttgcgccggt atcaaaagtt tcatcggaaa tttcaccaat
     6601 ggcattttgt tcttcattgg cggcaaagga gaatattgtc gcaatacgca gtggtttata
     6661 atttgcagat ttattagcgg cttcctcctg taagcgttta aacgtcgcgt aatagacttt
     6721 cgcagcatcc acgctgctta ctgccagcat ggcattaaaa cctctggaac ccgggaaagt
     6781 gcggtgggtc ttctggcgga agttattcag gatatattgc gtgatttcct gaatacgcat
     6841 gggatgaaga aacgcctgct gattttcagc cgcactcagt tttttctcgt cagtttctgt
     6901 ctctaaagac ttaaactgtg gccgcacatc gttgtagtcc accttgaatt tgagcacttt
     6961 ttcgtcacga atcgcatcgg taatcacata cgaatgcaat tcacgaccaa atacgctggc
     7021 ggtcgtttct gaacctgagg cgttttccgg gaaaataggt gtgccggtaa aaccaaactg
     7081 ataatagcgt ctgaatttct tcttcagatt tttctgcgct tctccaaact ggctgcggtg
     7141 gcattcatca aatataaaca ccacttgctg attgtataca ggcaggtcgc tttctgcttt
     7201 catcaggtta ttaagtttct gaatagtggt gacgataatt ttgttatcgt ccttatccag
     7261 atttcgttta aggcctgcgg tattttccga gccgttaaca ctatctggcg aaaaacgctg
     7321 atattccttc atggtctggt aatcgaggtc tttcctgtcg accacaaaga agactttatc
     7381 aataaagtcc agttctgttg ccagacgcgc ggctttaaag ctggtcagtg ttttaccgga
     7441 acccgtggtg tgccagatat acccaccgct ttccggtttt gaccagttct tcgctgtaaa
     7501 ggagctctta attttccaca gaatgcgctc ggtggcagca atctggtacg gtcgcatcac
     7561 cagtagcgtc tgactgctgt caaaaacgct gtagttcacc agaacattaa gcagggtatg
     7621 tttctggaaa aaggtggcgg taaaatcttt gaggtcttta atcagcgtgt tgtctgattt
     7681 cgcccagttc atggtgaagt caaaactgtt tttatcgcgc tttgtcgtgt tggcaaagta
     7741 acgggtatcg gtaccgttgg aaatgacgaa cagttgcaga tacttaaaca gggaattttc
     7801 gctgttaaaa ctctccttgc tgtaacgatg tatctggttg aaagcctcac gaatcgccac
     7861 gccacgcttt ttcagttcga tttgcaccag cggcaaacca ttaaccagga tagtgacgtc
     7921 ataacggtta ctgtgagaac ctgcctgttc aaactgccgg ataatctgca ctttgttgcg
     7981 catgagattc tttttatcta tcagatagat attctcaaga cgcccgttgt caaaaataaa
     8041 gtcacagata tagtcgatat ggattttacg ggtcttatcc agaatgccat cgccagggtt
     8101 gtccagatac tgctccgtga aacgccgcca ttcgctgtca ttaaacacca cgccattgag
     8161 gctctgaagc tgttcccgga cattagccag catcgccgac tgtgatttta cggaaataaa
     8221 ttcatagccc tgattctgca ggtcctgaat cagttcacgt tccaggtccg attcactttg
     8281 gtagctgtcg cctgtttgct cagccttgat gtacttatca agaacgataa agttactggt
     8341 ttcagcaatg gtgtgtgtct gaagaatcat agcgcatcct ttgtgccgtc tggcggggcc
     8401 ggaagggagt taagggtgac ttccggcgtg taaaaatggt ttatatactg accggatgtt
     8461 aaggtggccc ggtcggtagc aattgtcaat tagttactga cggtttcagg tttcgggaaa
     8521 ctgaacagta aatcacgata gtactcgtat tgtttctggc gcaactcgat ttcacgcggg
     8581 agaccttcgg tgatggagtt ggtcagggtg tcgaatttat cgagtatttc gacaatgcga
     8641 gcttgttctt ctgaaggtgg tacaggtatt gggaatttta aaaacattgg tcgacgaagt
     8701 gaggtaaccg atgaacttac tgatgctttt tgaatatagg taaaaaaata tgctgttata
     8761 tagtgatata caaatcgcgg attcataaat gcatttggga caatacgata tgctctctga
     8821 tgcaaagcat atttaccttt agcgtaatga aataccttcc ccaccccggc accatctcca
     8881 gccgtaatga ttgctgtttc atcaaaatca aatacattta actttaatgg ttcacgtcca
     8941 cgtgcataaa aaatatattt gccatgttct atagcatctt gcgtatcatg gttacctgtt
     9001 cctataacag ccacttcccc caaagtcttc cactcaatcc gaataccatc catggcgtcc
     9061 ggaaggatgt aattaccccc cccccgcaat atctttgaaa ttcaacaact tatctcgata
     9121 gtagttgtat tgttttttac gcatgttaag ctctgctgtc agctctgctg tcagggcgct
     9181 gaatttgtcc agaattcgca cgatttcaga ctggatggca agggattttt ccggattatc
     9241 cgggcagggg attggtacag gtatattttc aacctgatga ctcattaact taggatttcc
     9301 cattcctgag taaacatg
//
LOCUS       DAZGCQ010000162         9310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000162 DAZGCQ010000000
VERSION     DAZGCQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.162"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..105)
                     /locus_tag="ACHK07_004636"
     tRNA            complement(30..105)
                     /locus_tag="ACHK07_004636"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Lys,seq:ttt)
     gene            complement(270..1061)
                     /gene="cpoB"
                     /locus_tag="ACHK07_004637"
     CDS             complement(270..1061)
                     /gene="cpoB"
                     /locus_tag="ACHK07_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO8104870.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1071..1592)
                     /gene="pal"
                     /locus_tag="ACHK07_004638"
     CDS             complement(1071..1592)
                     /gene="pal"
                     /locus_tag="ACHK07_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO8104871.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1627..2919)
                     /gene="tolB"
                     /locus_tag="ACHK07_004639"
     CDS             complement(1627..2919)
                     /gene="tolB"
                     /locus_tag="ACHK07_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO8104872.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3052..4317)
                     /gene="tolA"
                     /locus_tag="ACHK07_004640"
     CDS             complement(3052..4317)
                     /gene="tolA"
                     /locus_tag="ACHK07_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO8104873.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4382..4810)
                     /gene="tolR"
                     /locus_tag="ACHK07_004641"
     CDS             complement(4382..4810)
                     /gene="tolR"
                     /locus_tag="ACHK07_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO8104874.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4814..5506)
                     /gene="tolQ"
                     /locus_tag="ACHK07_004642"
     CDS             complement(4814..5506)
                     /gene="tolQ"
                     /locus_tag="ACHK07_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO8104875.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5503..5907)
                     /gene="ybgC"
                     /locus_tag="ACHK07_004643"
     CDS             complement(5503..5907)
                     /gene="ybgC"
                     /locus_tag="ACHK07_004643"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8104876.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6057..6350)
                     /gene="ybgE"
                     /locus_tag="ACHK07_004644"
     CDS             complement(6057..6350)
                     /gene="ybgE"
                     /locus_tag="ACHK07_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO8104877.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     DLEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6350..6463)
                     /gene="cydX"
                     /locus_tag="ACHK07_004645"
     CDS             complement(6350..6463)
                     /gene="cydX"
                     /locus_tag="ACHK07_004645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO8104878.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6478..7617)
                     /gene="cydB"
                     /locus_tag="ACHK07_004646"
     CDS             complement(6478..7617)
                     /gene="cydB"
                     /locus_tag="ACHK07_004646"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8104879.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7633..9201)
                     /gene="cydA"
                     /locus_tag="ACHK07_004647"
     CDS             complement(7633..9201)
                     /gene="cydA"
                     /locus_tag="ACHK07_004647"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO8104880.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2263 a         2547 c         2239 g         2261 t
ORIGIN      
        1 cgctggatac tacaactaac taggttagtt ggtgggtcgt gcaggattcg aacctgcgac
       61 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
      121 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
      181 caacaaaaat ttcgatgtag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
      241 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgcgcctgt
      301 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
      361 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc tgcagccttt
      421 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
      481 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
      541 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
      601 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat caccgctttt caccggcgcg
      661 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgccgc accgctttga
      721 tcaccgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
      781 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
      841 cgcagggaat caatatcgga ttgattatca gagagttgtt gctggagttg ggttaaaagc
      901 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
      961 ccgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
     1021 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
     1081 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
     1141 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
     1201 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
     1261 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
     1321 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
     1381 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
     1441 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
     1501 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
     1561 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
     1621 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
     1681 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
     1741 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
     1801 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
     1861 accgttggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
     1921 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
     1981 ctgtggacga ccggcctggt cagaagtaaa tgccaggttc tggctgtccg ggaaccaggt
     2041 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
     2101 cattacgtac aggttcaggc taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
     2161 tggcgagaat gcaggcgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
     2221 gtttgccagc gtttggataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
     2281 tttagaaccg tctggtgacc acgccgggga catcagcggc tgtggtgaac ggtgaacgac
     2341 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
     2401 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaataccgg tcagtttttc
     2461 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
     2521 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
     2581 attgtaagaa ccatccggat tcggagtgac ctgaccgaca actacagcgt caatacccag
     2641 cgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
     2701 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga taccgccaat
     2761 atcttcaggt gccgcaccag gacccgccca ctggaaagga acaacaccaa taggacgacc
     2821 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
     2881 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
     2941 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
     3001 tccgtggcaa ccggtgcctg atgttgaccg tcggaacagt caacatcgcg attacggttt
     3061 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
     3121 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
     3181 tttgatatcc agtaacatgc catcgggtgc cagttttatg cgcagcgtac aggttttgcc
     3241 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
     3301 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
     3361 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
     3421 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
     3481 ttttgcagct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
     3541 tgctgccttt tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
     3601 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
     3661 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
     3721 cgcttccgct ttcttcttca gtgccgcagc ggctgcctcg gcttttttct gcgcttcggc
     3781 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
     3841 tgcttcttcc gcagctttag catccgcttc ggccttcgct ttagcatctg ccgctgcttt
     3901 cgctgccgcc tcttccgctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc
     3961 ctgctttttc tgttcctgag cagctaaccg ctctttctca agttgcttca ggcgttcctg
     4021 ttcagccgct tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg
     4081 ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac
     4141 cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc
     4201 ttctatattc tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac
     4261 tgctgaaata attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct
     4321 cggtttccaa aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg
     4381 tttagatagg ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca
     4441 gtgctttaat tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt
     4501 tcgggttggc cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct
     4561 caaggcgatc tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca
     4621 ctggcggatt atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct
     4681 ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta
     4741 cgtccagcaa cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg
     4801 ctctggccat ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa
     4861 tcgcggtaaa ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt
     4921 tgagacggtt ataagccatt accgccggaa tagcggcgaa cagaccgatt gctgtagcaa
     4981 tcaacgcttc tgcgatgcct ggcgcaacca tttgcagtgt tgcttgtttt accgccccga
     5041 gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga
     5101 tggagccaac cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca
     5161 tggaaatacg catcgcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg
     5221 cacgatgcag gcgcacaaat tctttgaacc cgctgtaaaa gatttgttcc gaaccggtca
     5281 gattatcccg tttcccctgg ctctcttgat agaggcgaga gagttcgatt ccagaccaga
     5341 atttatcctc aaacgcttcg gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga
     5401 taataatggc ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca
     5461 gaaggctagc cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc
     5521 gacaatagac ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag
     5581 aacctctgct tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa
     5641 agaggtgcca cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc
     5701 aggtgcgtaa tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag
     5761 cgcctgctga ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc
     5821 gacgtaactg gcgtggtaca ccacaccgcc ggcatcggta tcttcatagt agacgcgaac
     5881 cggccatcga aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc
     5941 ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg
     6001 agcgtaaaaa tttatgggcc tggcgcggcc cataattttt taaagagaat tctgatttaa
     6061 aagaagaaga aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc
     6121 ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg
     6181 gcccacatca gcaataaacc atgccagatt tccagatcac tggtcttcgc ggcgaaacgt
     6241 gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc
     6301 agggggcgct tgtccattac cgcatacaaa gtcgcgataa tcttactcat cagatgtctt
     6361 cttgaccggc tttgcctgat tcgacgtgct caagcgccag cgcggtgatt accccaaacg
     6421 aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta
     6481 gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca
     6541 gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat
     6601 gacgttaagc gtcagctggc tggaagttgc atcccacatt gtcaggcttg cgttcatcat
     6661 ggtgctggac ggcatcacaa acgggaacat tgcaatacca gcagtcagga tgatgcaggc
     6721 cagcgtcagg gaggagaaca caaacgccca tgcggcttta tccatacgtg cagtcaggat
     6781 ggtcagcagc ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt
     6841 gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagcg ggttagaggc
     6901 tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac
     6961 gccagccagt gcgaaacaga ccagtgtcac cagtgccgcc acctgagccg ttgcacgggt
     7021 acgcaggtgc agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat
     7081 catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcagct ggaagaagtt
     7141 accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt
     7201 accgaacgct acaccaatca ccaacggcgg aacgaagcta ccaatgaaga tgccccagtc
     7261 ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg
     7321 gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaaa acgcagcggc
     7381 atagaccatc ggccaggcag caaacagtgc gccgcctgcg gtgatcagcc aaacctggtt
     7441 accgtcccag tgtggtgcga tggagttaat cataattcga cgctcggtgt cgttacgacc
     7501 gaggaaacgg gtgagcatgc ccacccccat gtcgaaacca tcagtgactg caaaaccaat
     7561 cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg
     7621 acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg
     7681 accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc
     7741 caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc
     7801 ggtcagtgac gagttcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg
     7861 gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta
     7921 cagcgcggcg cgcagcagcc attttttctc gccaatgcgg ttgcggataa cactccagaa
     7981 agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcgaa
     8041 gtacagcggc gctacacgcg gaatggagtc tttggttgcc tgttgaatct gagcttcagt
     8101 cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccataaccga ggtctttctt
     8161 catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gctgttcgag
     8221 cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagttcttt
     8281 caggccgata actggagtat ccacggaacg cgttgcaatg atgcccagcg cgtaagggat
     8341 ctggatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca gagtaaaggc
     8401 agcaggcgca ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac
     8461 gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa cggcagccat
     8521 accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag
     8581 catataccat gcgctgatac cgaggatgaa catcgcgcca gtaacataac cagacgctac
     8641 agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat
     8701 ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt
     8761 tgcaaccaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca
     8821 catatgctga actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga
     8881 ttcgaggaag aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac
     8941 atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc
     9001 cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata
     9061 aatctgtttg ccggaaagga cgtagaccgt ttccataatg gccagcagga acgccatacc
     9121 gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga
     9181 cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga
     9241 accccgttag aaagggtcac acgcatgccc caatacaaaa ttatttgctc gcctctatcg
     9301 ttgccatttc
//
LOCUS       DAZGCQ010000163         9231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000163 DAZGCQ010000000
VERSION     DAZGCQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.163"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..102)
                     /locus_tag="ACHK07_004648"
     CDS             complement(<1..102)
                     /locus_tag="ACHK07_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO8104881.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAI"
     gene            complement(360..2759)
                     /gene="dosP"
                     /locus_tag="ACHK07_004649"
                     /gene_synonym="pdeO"
     CDS             complement(360..2759)
                     /gene="dosP"
                     /locus_tag="ACHK07_004649"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO8104882.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(2784..4166)
                     /gene="dosC"
                     /locus_tag="ACHK07_004650"
     CDS             complement(2784..4166)
                     /gene="dosC"
                     /locus_tag="ACHK07_004650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO8104883.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(4538..5857)
                     /gene="digH"
                     /locus_tag="ACHK07_004651"
     CDS             complement(4538..5857)
                     /gene="digH"
                     /locus_tag="ACHK07_004651"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO8104884.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5988..7523)
                     /gene="gadC"
                     /locus_tag="ACHK07_004652"
     CDS             complement(5988..7523)
                     /gene="gadC"
                     /locus_tag="ACHK07_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO8104885.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(7679..9079)
                     /gene="gadB"
                     /locus_tag="ACHK07_004653"
     CDS             complement(7679..9079)
                     /gene="gadB"
                     /locus_tag="ACHK07_004653"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO8104886.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         2376 a         2251 c         2230 g         2374 t
ORIGIN      
        1 aatagcttta cctgtgatgt tatcagcgca ggcgtatttc aattcgatct ccagatcagg
       61 gaagatcacg gctaaatcaa ccagttcggt ggtatccgac ataaacggtt cctgtataag
      121 acaaaaattg ctgcgctttc cgcttatgca gatctcatgc catgccggga taagcgccag
      181 aatgctggct taaagttata ttttttaact tttgatcaac atttgtgcag cgtagtgcag
      241 ttttggtgca agaggggaag ttaaggaagg aatctcccgg aatcgtagct gaaatcacag
      301 tatttaagtg acagtgtcac gttaaatgaa aacccgcgag tgcgggcgag aggaatttgt
      361 cagattttca gcggtaacac gctgctcatc cagcctggaa tttcttcggc gggtaggggg
      421 cgggaaaaga aatatccctg aataactcga cagtggatct tacgtagcat ctcaaattgc
      481 tctttggttt cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta
      541 atggcttcaa gtaaggcaag gatgcgtttt tccgtcagac aacgatcgac aaaacttttg
      601 tcaattttga tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgtg
      661 ccaaaatcat ctaccgataa acccacgccc atatcacgca ggatctgaat gcgcttaaag
      721 atttcggtat cgtgttccat catcatgctt tccgtgattt ccaccgtcag ctggtggccg
      781 tcaatacccc aggcttgcat tgcatcagac acctgattag gcagttgatt actgcgaaag
      841 tgcagcgccg acaagttcac ggataacgcc gggatatgaa tattctggct acgccattct
      901 gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc
      961 tcttctgcga gaggaataaa ccgtgaaggg ggcacatgac catgctgggg atcgtgccag
     1021 cgagcaaggg cttcgatgcc gtacagttca cccgtttctg cgaagatttg cggctggtaa
     1081 accagtttca gttggttatt gctaatcgcc tctttcagcg ctgcgcctaa aaccaaacgc
     1141 tcttttacca tttcgttcat cgccgggctg aagaactgcc agccgttacc gccattcttg
     1201 cgaatataat ccattgcatt gtgagcagtg gagagcaggt aatcgcggtt tttacccacg
     1261 tcgtagctga tgccaatact caaggtaagc gggaagggtt tatcgtcaat cattatcggc
     1321 ttgctgacca cattccgtag ctcatcggcg atttgggtaa tgttactgac gtcgttttcc
     1381 aggctcacga ggacaaactg cgtaccttcg atacgacaga gatactgatc cggtttgagt
     1441 ttttcacgaa agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg
     1501 ctatcaatca catcctgaat atggtcaaca ccgatgagat acaccaccgg agagacggct
     1561 ttgtcgacca ggtcatcgag gtaattgtgc aggttattgc gatttggcag accggtcatc
     1621 ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt tttcctgttc cagcgccagc
     1681 gcggccatat gctggctgat atctgccacg cgttcgataa aggcgctggt ttctgctcct
     1741 gacgaggttt taatttgcag gatccccgca ggcgcgccat cacgctgacg aatggtcgct
     1801 gaccagcttt gcgcattttg aacttctgca ccgtgggaag atgacgccca gtgtatcggc
     1861 atcccgttgc gcagtgcgaa cagcgaaaca tgcgattcgt tgagaacaga ttcgatatta
     1921 cgacaaatga tttcccccat ttcatgaaat ggcgggctgc tgcacatggc ggcgagaata
     1981 ttgccttcaa gctggcggat ttgccgttct tcggtgatat ccgagaaggt cattaccagg
     2041 ttctgcagat gcgcgagcac gtcataaacc gggctgatag aagctttaat ccagattttt
     2101 tcaccggtgc gcgtcaacag cagaaattcg tcctgatcgc gggcggtttt ccatagcaac
     2161 tgttgtaaac gaatgcggtt atcggcaggg aattcaggaa tgttcaggag tgtatcgggc
     2221 tgcataccgc tggcttcgct aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac
     2281 tgcacaatat ggcgttccgg atcgaggaca atcaccggtc ggtcgagatg gtcaacggca
     2341 ataatcaatt gcctggtctg ttctttttgc gccatttcta cgctggcatc ccgtaccagc
     2401 gccaggtaat aaactttccc ctcggcgctc acttttgata gcgcaaaacg ggtccagatt
     2461 ttactgccgt cttttttctc cagctgcagc tcccgactca tcccctcgac gcgcgcttta
     2521 ccgccttcac ggttatgacg aatgtattca ggatgcgcag gacgcaaatc ccgcggaatc
     2581 agcatatcaa tgttattgcc aatgacttct tcacgtttgt acccccagag cttctctgcg
     2641 gcggggttga aaaacatcac ttcatcattt tcgttaatta acaccgcacc catcatattt
     2701 tgctcaaggg cggggaaaaa aatgccatcg gcggcattat ccgcatcggt tagcttcatg
     2761 attacctctg catcctggcg catctaaaga ctggctttcc agagttcaac acggtttcta
     2821 cctcgtcttt tggcgatata cagagcttca tcggctattt gaatgaggcg ctcatagtca
     2881 ggatgaccat taaacatggc ggcaccgatg gaaagtgaga gggcaatatc ttcgccgttt
     2941 gcggctttca gtttggtttt ctccacccga ctgcgaatac gttctgcggt acgtaacgtt
     3001 tcgttttcag aagcttcagt caaaacaatg ataaattcat cgcccccgta gcggaaaaca
     3061 taatcactac tgcggacgtt gtcataaaag gcctgagaga ctttacgcag aatttcatcg
     3121 ccagtgttat ggccccacgt atcgttgatc tctttgaatt tatcaacgtc aataatcagc
     3181 actgacagcg gtgtaccggt ccggttggca tgggcaattt cgcgtttgaa gatagtcggc
     3241 aggaaacggc ggttaagtaa tttcgtcagt acatccatac cgacttcgtg gcgcgatact
     3301 tcttcaaaca attcacgcag caaggtaata atttgcgata cggtatttct tatctgtaat
     3361 aaaaatttca cccgcagact tctgttattc aaattcctgg tgttacgcat ggtttgattg
     3421 aaaataccgt cgaaatcctg aatcagacga gagatatggc ccacttcagc aatgccgctg
     3481 aaataatgtc gacctttatg gttaaaccac aggccaaaat cagcctggct taaaggcaaa
     3541 ctactgccta aatcagaatc cagcaggatt ttatagatga tatctatttc ccatgaaagt
     3601 attgaggcta tttgccgttc tttttcttct tcggcgtttt ccagtaacga gaagatacga
     3661 tagttttcat cttcttttga ggcactactg tcactaaagg taaacgcgcg ggtcatcact
     3721 tccatagcga tatcaatact gttaatcgag aaatggtaga cctgaagttt ttctgcggca
     3781 gaataatccg aagagaagat taccggatag aggatctttt tcagcacccg aaaccccatc
     3841 tcgacaattt ctaccggaat tcctatgcgg gcatgcactt ccgcgacggt atgctggatt
     3901 tgtattagcc tttcgacatc gtcaacctgg gcagaaagca cgttaataat ccagcgttcc
     3961 atcgcacttt tcaactgccg ctcaacttgt tcattactca agaattcttc ggcatgcggg
     4021 tcgatgcgga caattcgata aaactcaata ctcagataat gagcatgcgc aacggcaatt
     4081 tccgcggctt tagctcgaat gggcggatct gcctgttcga caagtccggt ccactcatct
     4141 ttcattcttt taaaatacat ctccataatt cacaccctta taaggctggg aaatcagacg
     4201 gaatcaaaac gcaacgtgcg agatcgacta actgcaccat attctcctga aatatgaaga
     4261 tatactgaaa agaaataagc gacttaggac agtttcaatc tacgctactg ttcttcagaa
     4321 gagtatagcc catcgtaatt atttttcggt gacagcgaat atcgtatgtt ttttcatatt
     4381 catacatttg tattagggga tttatggctg tttaactaag tgtggttaat tgcatggtta
     4441 atttgacttt agtaagcatg attagtagtg ggatagttca agagggtaac aagccggtgg
     4501 gtaatgtacc ggcttgttac aaagtaagaa tgggagttta actgccccaa cgactttgca
     4561 gatagctgac cgcttgttga gtctgcggtt tattcagata gtcctcacgg aacaagatgg
     4621 tgccgctaat ttctggtacc gcatcgttaa gatcgagctg ctttttcagt tccggtacgc
     4681 cgccgttaat catccagtct ggctctatct ttgaaggttc acccacttta tagaaggcga
     4741 taccgatata caggcgggtc ctggtcggtt taacgacatc cgcccaccat tttgccaaca
     4801 cgtcataacg cgcggcactc cgtgagaacg gccagtaaat ttggggagca atgtaatcca
     4861 gcaatccttg ttccacccat cgacgggtgt cagcgtagga ttcgtcatag gctgccgcgc
     4921 ctcgggtatc ggaaccgagc ggatcgtgtg atcggttacg ccacacgcct gccgggctaa
     4981 caccaaattc gactcccggc ttaatgcttt taatggtgtg cgatactttt gcaattaact
     5041 gctgagtatt gttgcgccgc cagtctgctt ttgacgcaaa tgcgcctccg tatttgcggt
     5101 acgtttcgtt atcatttagc cgtgaacccg gtgactcagt atagaaatag tcgtcaaact
     5161 gcacgccatc taccggatag cgggaaacca cttctgcgac tattgatgtg atccagtcct
     5221 gaacctcagg gatgcccggg tcgaggacaa agcgatcgcc agacgttctg atccagtcac
     5281 ggtgttgcac atagacgctc gccggttgtt gagacagagt gctattcagt tccctgatag
     5341 taccgggctt cgtattaacc gatacgcgat aggggttaaa ccaggcgtgt actttcatcc
     5401 cacgcttgtg ggcttcgtcg agcataaatt gcagcggatc gtaacccgga ttttcaccaa
     5461 tcttaccggt cataagatcg gaccatggca aaatttttga aggccacagg gcggtaccgt
     5521 ccggcttgac ctggaaaaag accgtgttta taccgagacg ttgcaaatga tccaatttgt
     5581 cgatcatcgc ctgttgttgt acacgggccc ggctggtggg gttactaatg ttaaccgagg
     5641 aaaccggtgg ccagtcgagg cgagacaccg tggccagcca gatgccacgc atcggttgtg
     5701 acgattgttg cgtcgtggct ggtggctttg aacccgctgg tggtgtcacc atggactctg
     5761 gaggcgtgct tttacaacta cacagtaaaa gtgccagtgc aaccagtatc gctggtcttc
     5821 taatcgttaa tttcttgttt cgggagcaga tatccatatg tgctggtttc cggtaaacag
     5881 atgtgcgctc attctcgtac ttatcccggt taagtcaata cgacagcaag cacgaaaaag
     5941 ggagcgatga attatcgctc ccttgtctta taaccattca gacatggtta gtgtttcttg
     6001 tcattcatca caatatagtg tggtgaacgt gcacgcgggt gcaggaagaa gtgacctttt
     6061 ggtgcgttct gactgttgat tggctccaga gtgacgccgg tattcgcttt gcctttacga
     6121 tcatgaacag catagagaat aaagggcagg gcaagtacca ccaggaaact aacgaccagt
     6181 aattcaacat acatatcggt agagtcaccc tggatattat ccggcggcag gaaggaaaca
     6241 ataaacgcca taattgaagt cagcagaccg acaatagcca cgaccagttt cacaccttta
     6301 ccaccaggga tattaaatgt gcgttttaag tcaggatgtt taagaaccaa cacaatgtag
     6361 ccaataaaca gcatgaaata agcacacaga taaatcacca ccgtcagcgc cagtgcgatc
     6421 aggaaggaca tgttgttacc gccaccggta ttggtgagga tgatcaacgc gatagaagta
     6481 atcaccagct gtgaaatgac cagcgttacc ggtacgccat ttttgttcat tttagcgaat
     6541 gccgctggca gcaggttttt ctgcgctgtg acatacatcc cgcgagaagg accaacaatc
     6601 caggaggcga tttccgccag aacacccagc aacagcagtg cggagatcac gcgaaccgtc
     6661 cactcaattt ccggtgccac atgggacatc agaacggtaa aggtttgcat tacccctgcg
     6721 gagaggttga tttcattacc cggaatgacc atcgcaatag acaaaccgcc aacagagctt
     6781 aagcagattg ccgccaccat cagcagtaac atagccagtg gatagtcgcg ccctgggtta
     6841 ctcatttcat tgacgtgggt tgcggaagct tctacgccca tataactcaa aatgaaggca
     6901 acaaatacaa ccagggtgcc cactttagag aagtcaggga agaaggtctt cgaatccatt
     6961 tcgatagcaa cgggggcacc ggagtgcaga taaatagccg ctaatgcaat caaaataaat
     7021 gcaggtaaca ggataccggc gaagaagcca actttagcaa ttcgcgccgt gtatttcgtg
     7081 ccaccaaact gcgttaatgc cagcgcccaa agaatgatga gtgctgcaat agttttggta
     7141 atggggtctt cattcagcgc tggccatttc agaatgtagg agagtgcccc taacacgaaa
     7201 tagagcatcg gaataaaacc aatggcgatt tgcagatagc caaatgagat cgctgcaaat
     7261 ccccatctcg gccccagagt atttgatacc caggcgaaga caccaccttc ttcccagccg
     7321 tcgacggtgg ccatttccgc agcacaaagt cccacgggaa taaaccataa aatcccgcct
     7381 aatagcagga agaagactaa tgaaaagccc gatgttgcga aggtagggta ttcataaaca
     7441 gccattacca tcgatgccgt tatggcaaaa aagccgagta atgtgagctg cttagcttta
     7501 cctgtctgta ctgatgtagc catattatcc ccctaaaacg gtattcctgt cggaaccgca
     7561 cctgttttgt tctgaagcgt attcagaaca atattttccg ttgctaatgc cagtgaacag
     7621 actttggaaa ttgtcccgaa acgggttcgt ttcgggacac cgttaccgtt aaacgttatc
     7681 aggtatgttt aaagctgttc tgttgggcaa taccctgcag tttcgggtga tcactgagat
     7741 atttcaggga ggctttgtag tcttccagca acagttcagc aaagtccatt tcgaagccgc
     7801 gacgacacat aatgcgcatc accacgatgt cggtggcttc accgccgaga gtgaaggccg
     7861 gaacctgcca gccgcgcaga cgcagacgtt cagagaggtc gtacagggtg tatcccggat
     7921 cttcaccctc tttcagtttg aagcaaaccg ccgggatgcc ttcgtccggg cgacccgtac
     7981 agatgaactc atacggcccc agtttggcga tttcatccgc cagataagcg gcaacctggt
     8041 aagaggcgtt ctgtactttg gtatagcctt cacgaccgag gcgcaggaat tcatagtact
     8101 gtgcaattac ctgacccgcc gggcgggaga agttgatggc aaaagtacca atttgaccac
     8161 ccaggtagtc aacgttgaac accagttcct gcggcagcgc ttcttcgtca cgccagataa
     8221 cccagccgca gcccagcgga gccagaccga atttatggcc tgaagcactg atcgatttca
     8281 cacgcggcag gcggaagtcc cagacgatat ccggggcgac gaacggtgcc aggaagccac
     8341 cgctggcggc gtcgatgtgc atgtcgatgt cgataccggt gtcggcctgg aatttatcca
     8401 gcgcatcgtg cagcggttgc gggaactcat agttaccggt gtaggtcacg ccgaaagtcg
     8461 gcaccacgcc gatggtgttt tcgtcgcagg cttcaatcat gcgtttcggg tccataaaca
     8521 actgaccggg gcgcataggg atctcacgca gctccacatc ccagtagcgg gcgaatttat
     8581 gccagcagat ttgtaccgga ccgcacacca ggtttggttt atccgttggt ttgcctgcag
     8641 cttccatacg cttgcgccaa cgccatttca tcgccatccc gccgagcata caggcctcgg
     8701 aagaaccaat ggtgttggtg ccaacggcct gaccattttt cggcgcaggc gcatgccaca
     8761 gatcggcaac catatttacg caacgcaggt cgatggctgc ggattgcgga tattcttctt
     8821 tgtcgatcca gtttttgtta atggataaat ccatcaactt gtggacattt tcgtcgtccc
     8881 aggtctggca gaaagtggcc aggttctgac gagcgttgcc atcaagatat aattcgtcat
     8941 tgataatctg aaatgcgaca tcgtcgcgca tttcgtgcag cggaaaacgt tttgattctg
     9001 cgatagtgga aatagacttc gcaccaaaac gtgaatcgag tagttccgac cttaaatccg
     9061 ttacttgctt cttatccatt ttaaactcct taaaatgatt ggatcgcatt aaaaaagtag
     9121 gatttatcga taaagtaagc aagtgcaaag gactcgtgtt taaataacaa aatcctaatg
     9181 ttatttatgg tgagatattg cgtgaataat attttttcat tgaaaaacaa t
//
LOCUS       DAZGCQ010000164         9205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000164 DAZGCQ010000000
VERSION     DAZGCQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.164"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..877)
                     /locus_tag="ACHK07_004654"
     CDS             complement(77..877)
                     /locus_tag="ACHK07_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104887.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKAYVSMVKDLVSRFQ"
     gene            complement(1205..1281)
                     /locus_tag="ACHK07_004655"
     tRNA            complement(1205..1281)
                     /locus_tag="ACHK07_004655"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Asp,seq:gtc)
     gene            complement(1414..2145)
                     /gene="dnaQ"
                     /locus_tag="ACHK07_004656"
     CDS             complement(1414..2145)
                     /gene="dnaQ"
                     /locus_tag="ACHK07_004656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO8104888.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2210..2677
                     /gene="rnhA"
                     /locus_tag="ACHK07_004657"
     CDS             2210..2677
                     /gene="rnhA"
                     /locus_tag="ACHK07_004657"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO8104889.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2674..3396)
                     /locus_tag="ACHK07_004658"
     CDS             complement(2674..3396)
                     /locus_tag="ACHK07_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8104890.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3430..4185
                     /gene="gloB"
                     /locus_tag="ACHK07_004659"
     CDS             3430..4185
                     /gene="gloB"
                     /locus_tag="ACHK07_004659"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO8104891.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4257..5615
                     /gene="mltD"
                     /locus_tag="ACHK07_004660"
     CDS             4257..5615
                     /gene="mltD"
                     /locus_tag="ACHK07_004660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO8104892.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5663..6286)
                     /locus_tag="ACHK07_004661"
     CDS             complement(5663..6286)
                     /locus_tag="ACHK07_004661"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8104893.1"
                     /translation="MSGLPQGRPTFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKN
                     ITTRHGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASCEWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQNDGSFTSDIIMVEAHKAA"
     gene            complement(6290..7090)
                     /locus_tag="ACHK07_004662"
     CDS             complement(6290..7090)
                     /locus_tag="ACHK07_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8104894.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7331..8245
                     /gene="yafC"
                     /locus_tag="ACHK07_004663"
     CDS             7331..8245
                     /gene="yafC"
                     /locus_tag="ACHK07_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO8104895.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGALTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(8242..9045)
                     /gene="dkgB"
                     /locus_tag="ACHK07_004664"
     CDS             complement(8242..9045)
                     /gene="dkgB"
                     /locus_tag="ACHK07_004664"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO8104896.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVSRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2497 a         2172 c         2164 g         2372 t
ORIGIN      
        1 catatttaca ttatagaaat actcaactcc tgtttgctgc caccctaaac tacaggcggc
       61 agcataacaa taacgattac tggaatcgac tcaccaagtc tttcaccatc gatacatatg
      121 ctttttgttt caaattaaac agttgcgctt ccgtaacatg gtcatttgat agttctttat
      181 tgctcgtttt tgtaattcca gccggcggaa aaattactct cgattcataa ttcaccggcg
      241 accaggtagc ccactcacct gttgccacat ccaccagcgt gaaacgtacc aggtagcgca
      301 gagagatagc gtcagtgagt ttttcatttt tatagtcgga ccatactgtt gatttcagcg
      361 cagagtcata ttttcctgtc tgcaacatat cctggtaaac aataatcgct ttctgatgtc
      421 cttttgcagc cacaaaacgc agtgcctgca tccagttcat attctcagca cgggcaacat
      481 cctcgttctc atttttatct ttgtttttgt tacaagtcag aggcttttgc ctgtccggga
      541 taccagagaa tgtggaaaca gtataatatt ttcgcatctc ctcctgcata atggtttccg
      601 gtgcgcggtt gccggattga acaagtatta caggagaatt aaggggaacg cggaactcat
      661 ctccatcaag cgctgcttga atatcctctt cggatacggc cagagtggtt tcattaccga
      721 ataaatcttt gtctgtcagt tgctggggag tgtttttatt aatattactt tgtgtgactt
      781 gagctttttt cttcttttca tcaatagaag attcagaggc acatcctgca agcagcgaga
      841 ataacacaca tatgcaacaa agcttttttg aattcataat tggacactcc ctcgcctgtc
      901 ataatgatta gaatatttag cattgataac ggactctaat tatatacccc acttaaataa
      961 taacaaacaa aatcaatttg aaatatacat tcatttaatc aatataaatt tattctctgg
     1021 aaatatattt aagctgaatg gttcgatatc attattgaat tttaatgaat gagacaacaa
     1081 gcgagaatca cgcacttacg ccagagtaaa atcattgaac agagaaatgg tggaattcaa
     1141 atacaaaaaa accgcaaaat taaaaatctt gcggttctct gaactcattt tcatgagtga
     1201 atagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
     1261 aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat
     1321 tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat catttgctgc aaaaatcgcc
     1381 caagtcgcta tttttagcgc cttttacatg tatttatgcc cgccagaggc agcttccgcc
     1441 ctttttctgc accagatcga gacgggcttc atgtgctgca agctcttcat ctgtcgcaaa
     1501 aacaacgcgt aacttacttg cctgacgtac aaggcgctgg attgttgctt caccttgttg
     1561 ctgttgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag
     1621 ataaacttcc gcaaggatct gggcatcgag taatgccccg tgcagcgttc gtttactgtt
     1681 atctatttcg tagcgagcac ataacgcatc gaggctgttg cgcttaccgg gaaacatttt
     1741 cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg
     1801 cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac
     1861 caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt
     1921 atcgagcaaa aattcatcgg caataccatg tacaccaaag gcttccggat ccaccagccg
     1981 atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac
     2041 ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt
     2101 ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt
     2161 cagacttgtc gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca
     2221 ggtagaaatt ttcaccgatg gttcgtgtct gggtaatcct ggacctgggg gttacggcgc
     2281 tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa
     2341 caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga
     2401 agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt ggatccataa
     2461 ctggaaaaaa cgtggctgga aaacagcaga caaaaaacca gttaaaaatg tcgatctctg
     2521 gcaacgtctt gatgctgcgc tggggcagca tcaaatcaaa tgggaatggg ttaaaggcca
     2581 tgccggacac ccggaaaacg aacgctgtga tgaactagct cgtgccgcgg cgatgaatcc
     2641 cacactggaa gatacgggct accaagtcga agtttaagcc tgtggtttac gacattgccg
     2701 ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca tcggatttag
     2761 cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat
     2821 atgcgcattc aaaagttttc ctccgtgttt gttccacggg agaacgtgga aacggctggc
     2881 gtgtagcact tcaaaattca acaaagagag ccagtccagc tgccgcatca gagtaaacat
     2941 ccggctgtta tagggcgagg ttttgcgcaa taccggcaca agtttgcgta atcccattaa
     3001 actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggc
     3061 ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac
     3121 atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct ggacgggcat
     3181 tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc
     3241 gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag
     3301 ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc agcgatcagg
     3361 agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactgag
     3421 aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg
     3481 aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac
     3541 gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat
     3601 cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca
     3661 caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt
     3721 ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac
     3781 ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtcggttg
     3841 tttgaaggga cagcatcaca aatgtatcaa tcacttaaaa agttaagtgc gttacctgac
     3901 gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt
     3961 attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca
     4021 aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta
     4081 agaacagaag atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct
     4141 gaagaacgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc
     4201 atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga
     4261 aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg
     4321 ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa
     4381 gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt
     4441 tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac
     4501 agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata
     4561 tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac
     4621 ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct
     4681 ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgatg
     4741 cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag cgtctgaaca
     4801 aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg
     4861 tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac
     4921 cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca
     4981 aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc
     5041 gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg
     5101 tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtggtc
     5161 cgcaatacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctggcttcag
     5221 gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg
     5281 tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc ggcgtaagca
     5341 ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt
     5401 tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat agcattacgt
     5461 atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca
     5521 aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat aagctgacgt
     5581 tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa aaaggcaccg
     5641 attcccccgg tgcctttttt atttatgccg ctttatgtgc ttctaccatg atgatatcac
     5701 tggtgaaaga gccatcattc tgcaaggaaa aatacgttct cacctctgtc gatgcgctct
     5761 gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc acgacccatg
     5821 aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat
     5881 tgattaacgt caaccactca cagctggcgt agtttcgtac gtgagaggta tcgcgtaatg
     5941 cttctaccgt ctgtaaccag atgtcgcgca ctgggtggcc cggagacatt acgtccatca
     6001 caatcagcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct gcgccgacat
     6061 catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg
     6121 gcagactttc ggcataacca tgacgagtag tgatattttt cagttgccgg gcttcggcag
     6181 cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc gcgctcacgt
     6241 tttgcgcagc gacaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag
     6301 gcttgccggg actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccagta
     6361 cagaagcttc actgacgttc agaccacggt agaaaacaaa atcgagcggg cgaccaaacg
     6421 cccgacggcg ctgatcatcg gtaaaacgca cctgacgcag cgacatttcc cgcgcaaaac
     6481 gatataacgc gttcatcctt ctacggctcc aggcattgaa atctcccgcc ataatgaccg
     6541 ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa ctgcttacta taaacatcca
     6601 cgcccagact gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta
     6661 atggatagac cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta
     6721 acgggcagca atacactgga tgtgccgccg aaagggtcat tacgccagaa ggatgttgtg
     6781 gcagcacgaa agcgggtacc tgatcggcgg caagatagtt agcagtcgca aactgtacca
     6841 actctggcgt tgtttgcgct tcctgcaata acaccagatg tgcgtctttg ccgtaattct
     6901 ttaataccga caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa
     6961 tacgctcttc ggtacttaac ggttctccgg gtggtaatgc ctggccgatg ctcgcaaaag
     7021 accccggcgg taagatcctc tccgcaggtt gtccggcaac atagcgcatg gcataggtgt
     7081 tttttcgcac ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt
     7141 tctactgtta tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa
     7201 gcaagtgctt acaccggtca gaattttgct cattattgag aacattaaat cggatgacat
     7261 atcacgcatc ctgatggttt aatgacaaat aaattcaaaa tatctcatct attttgcaac
     7321 aggagcaaaa atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtcgaaag
     7381 cggcagcttt agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc
     7441 ggtgaaaaag ctggagatga aacttggcgt tagcctgctt aatcggacca cgcggcaact
     7501 tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt tgcaggagat
     7561 ggcagcggca gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttgcgcat
     7621 cgatgccgca actccagtgg tgctgcactt tctgatgccg ttgattaagc ctttccgtga
     7681 acgctatccg gaagtcactt tatcgctagt ctcctccgaa acgattatta atttgatcga
     7741 aagaaaagtg gatgtcgcga tacgcgctgg tgcgttaacg gattccagct tacgtgccag
     7801 gccgttattt aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg
     7861 gaagccagaa acgatcgacg atttaaagca acatgtttgc ctgggattca ctgaacccgc
     7921 ttccctcaat acctggccga tagcctgtag cgatggacaa ttacatgagg tgaagtacgg
     7981 tttgtcatcc aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc
     8041 gtgtttgtcc gactatatga tcgacagaga aatcgctcgc ggagaattgg tggagttaat
     8101 ggcagataaa gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc
     8161 ggtaagtacg cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc
     8221 cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag
     8281 gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt
     8341 ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa ttactgagta
     8401 accttccccc atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc
     8461 gatacgagca ataacctcat ctttcagggc cttaccatac gccagcgtca tataggaagt
     8521 aatatggatg ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga
     8581 gagttcaatc tggttagtag cgatgttttc agcgccaaca gcagcaatcg ccttttccat
     8641 caatgggatc gtgaagttgg aaataccgat ctcacgcgtc aacccttctt ttttggcttc
     8701 cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat
     8761 tagagtcaga tcaacataat cagtacgcaa tttttgcagg ctctctttca gactcgggat
     8821 caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg
     8881 tgacacgcca ctttctgcaa tcgcctgacc tacggcagct tcgttatcat agatttgtgc
     8941 ggtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc
     9001 gtctttcaga cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta
     9061 atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggaa aaagagcgta
     9121 aaacgcagag gatttttgcg attttagcaa aaatagaaat gtagacgaaa aaaaacctcg
     9181 agcgataact cgaggcttct taata
//
LOCUS       DAZGCQ010000165         9158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000165 DAZGCQ010000000
VERSION     DAZGCQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.165"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..570
                     /locus_tag="ACHK07_004665"
     CDS             <1..570
                     /locus_tag="ACHK07_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO8104897.1"
                     /translation="AFQHYCQSLEQIEDRILQLDQEISRLLPEWSLCNLVCQLQALKG
                     VGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRPRGITKVGNSELRRL
                     LYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVA
                     ITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(823..2247)
                     /locus_tag="ACHK07_004666"
                     /pseudo
     CDS             complement(823..2247)
                     /locus_tag="ACHK07_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
     gene            complement(2278..2628)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004667"
     CDS             complement(2278..2628)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8104898.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            complement(2625..2987)
                     /locus_tag="ACHK07_004668"
     CDS             complement(2625..2987)
                     /locus_tag="ACHK07_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8104899.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNEGRVSRRLP
                     VTTSSDTGVELLPVEITPDEQKEPVVAIAPSLSTTTQTRVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            complement(3103..3309)
                     /locus_tag="ACHK07_004669"
     CDS             complement(3103..3309)
                     /locus_tag="ACHK07_004669"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8104900.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVFVARIAREHDIND
                     NLLFKWLRLWLIKGKRKPTAVCSH"
     gene            complement(3440..6121)
                     /locus_tag="ACHK07_004670"
     CDS             complement(3440..6121)
                     /locus_tag="ACHK07_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO8104901.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDGEVMLTCHFRSGKKYTRP
                     LSVCQITPTVILRNVFLERKGNAVTSRAERVIIYGDKYAAVYYRESERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVADERVFYARGNNKNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLMAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKSIREKQISLNEYEKVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMSFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHRELLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
     gene            complement(6795..7319)
                     /locus_tag="ACHK07_004671"
     CDS             complement(6795..7319)
                     /locus_tag="ACHK07_004671"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO8104902.1"
                     /translation="MAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLL
                     QQLRGLFPGNPHVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQR
                     GKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYGEARNQNGSEQQQKQAELALNQLIN
                     RYQMEHTGNQERQP"
     gene            complement(7371..7520)
                     /locus_tag="ACHK07_004672"
                     /pseudo
     CDS             complement(7371..7520)
                     /locus_tag="ACHK07_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7845..8159)
                     /locus_tag="ACHK07_004673"
                     /pseudo
     CDS             complement(7845..8159)
                     /locus_tag="ACHK07_004673"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(8214..8519)
                     /locus_tag="ACHK07_004674"
     CDS             complement(8214..8519)
                     /locus_tag="ACHK07_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104903.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(8572..9021)
                     /gene="tssE"
                     /locus_tag="ACHK07_004675"
     CDS             complement(8572..9021)
                     /gene="tssE"
                     /locus_tag="ACHK07_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8104904.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSEEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMCVLSDVLLKYEPRIKRVDVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            complement(9024..>9158)
                     /locus_tag="ACHK07_004676"
     CDS             complement(9024..>9158)
                     /locus_tag="ACHK07_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8104905.1"
                     /translation="MFLEPDRKKNTWRVVLSRDELEPDTPRLIEVSGNTLTLLPVKDK
                     "
BASE COUNT         2327 a         2319 c         2035 g         2477 t
ORIGIN      
        1 gcttttcaac actattgtca aagtctggag cagattgaag atcgcatact tcaactcgac
       61 caggaaatat ctcgcctgtt acctgagtgg tctctgtgca atctggtctg ccagttgcag
      121 gctctaaaag gtgttggtca gctaattgca ataaccctgg ttgctgaact gggtgatttt
      181 tcgcgatttt ccaatcccaa acagctcatg gcttttctgg gactggtgcc aggtgaatat
      241 tccagtggaa acagtattcg tcccagagga ataacaaaag ttggaaacag cgaactgaga
      301 cgtctgcttt acgaagccgc ctggtcttat cgtacacctg caaaagttgg tgcatggctt
      361 atatattacc gaccggactc tgtaacacaa tattccaaag atattgcatg gaaagctcaa
      421 caacgattgt gttctcgtta ccgaactctg acagcaaaag ggaaaaaatc acaagtagcc
      481 attacggcgg tggctcgtga gttgactgga tttatgtggg atattgcact tgctgcccaa
      541 tcatcattca gtcagcagaa gcaaaattaa accctgaagc agtcagacac ggaatgagtg
      601 atcctcgaaa aagaattcag ggaagccaga atgcgctaat acctgcacgt agaaagaggc
      661 cagctcactg cgtaaatcgt catggggcta aaatccccga ataagagttt gtaaaatcca
      721 agatacagtc tgactgtttc aggttcttta atccgcgata taacgaagct cccggagctg
      781 cgtaggtctt tttgggctta atatttgaca aaggtcataa gagtttttct ttccgagaca
      841 gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac cgtcatcact
      901 gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc ccagtctgga
      961 ttttttcgac agcgtaccat tcttctccac catccattca tgcagcgacg tcagtaacac
     1021 tttgcttcgc tgctgcctga cggaaagacg ctctgactcc ggtaatcccc gtatttcatc
     1081 ctcgatggcg tacagttcac tgattcgctt cagggcttct tctgccttcg cacttttgct
     1141 gctgatgtat acatcgtgga tttttcgccg ggcatgggcc cagcacgcaa cttctgtcag
     1201 tgcaccacct tcacgttctg cactgaacaa cctgtcgtaa cctgtgaacg catccgcctg
     1261 caggataccc cggaaggggc ggaggtgttg ctccgggtgt ttcccctgcc ggttcggcga
     1321 gtacgcgaac cagaccgctg gaggagatga cgaacccaca ttgcgatcat cccggacata
     1381 cgtccagata cgccctgttt tcgccttttt ctgacccggt gccagtacct ttaccggtgt
     1441 gtcatcagtg tgaaccttgc gggtgttcat tacataacgg tacagggcat cattcaccgg
     1501 tgtcattaac tggcagcacg cgtcaaccca gttggagagt aaggcccggc tcagttcgac
     1561 accctggcgg gcaaagattt cactctgacg atacagtggc agatgttcgc agtattttcc
     1621 cgttaacacg cgggcaagta atccggggcc cgcgatacca cgctctatcg ggcgggacgg
     1681 cgccggtgct tcaacgatgc agtcacattt tgtacaggct ttttttaccc gttctgtgcg
     1741 gatcactttc agggcactgc tcaccagttc cagctgctca gcgctgactt cccctggata
     1801 atccagctca ccgccacact ccgggcaaca gctttcttct ggctccaggc ggtgtatttc
     1861 acggggaagg tgtaccggta acggacgacg atggcgcgac tgtcgcaact ggcggggaac
     1921 ctgaggatcg tcttcccgcc cactgtaacg atcgctgtcc tgttcacgtt gtttcagcag
     1981 ggcctcagcc agttcaactt cacgacgcag tttttcagaa cgggtaccga acagcatccg
     2041 gcgcagtttt tctatctgag cccgcagatg ttctatttcc cgttcatctt cttcgatctt
     2101 ttcttcggca cgtgtcagtg cagagcgcag gaaggcttcc gtctcttcaa ccagactcag
     2161 ttgctggtct ttctgacgga gggcttcagc ctgctcagag agcaaccttt ccagctctgc
     2221 gatgcgaatg aggtatttct gactcatgac cgtttttata atgcggtcag gagtttttta
     2281 caacattgtc agtgagttac ggcgggatgt ttttggctga cgccagtcca gcttatcgag
     2341 gagcattgcc agttgcgagc gggtaatgga taccttgccg tcacgtaccg caggccagat
     2401 aaactggcct tcctccaggc gtttggtgaa caggcacaga ccatcagcat cagcccaaag
     2461 aattttaacg gtgtcacccc gtcggccacg gaagataaac aggtgaccgg agaagggatt
     2521 atcattcagc acatgttgta cctgttctcc cagtccgttg aaggatttac gcatatcggt
     2581 aacgccggca acgagccaga tacgggtacc tgatgggagt gagatcatct tcccctcccg
     2641 gtcagttcac ggatcaacac cgtgagcagc tctggcgatg gattttccag cgtcatgtta
     2701 ccgtgacgga attccacctt gcaggagctg gcactgactc tggtctgagt ggtagtggat
     2761 aaagatggcg caatggccac cacaggttct ttctgctcat ccggcgttat ttctacaggt
     2821 aataattcaa cgccagtgtc agaagaggtc gttaccggaa gacgccgcga aacacgccct
     2881 tcgttctgcc agagcctgag ccatttgaac aggaggttat cattgatatc gtgttcccgg
     2941 gcgatacggg caacagaggc tcctggttgc aaagccagtt taaccatttg aagtttaaaa
     3001 ttatttgaaa atgatctgcg gggttctgcg gataatgttt tttgctccat aacaggcgtc
     3061 cactagtttg aaaaggtggg cacctacgtt accaatactg gcttaatggc tacatacggc
     3121 agtcggttta cgcttacctt tgatcagcca gagcctgagc catttgaaca gaagattatc
     3181 attgatatca tgctcccggg caatacgggc aacaaaaacc cttggttgta aagccagttt
     3241 aaccatttga agtttaaact catttgaaaa tgacctgcgg ggttctgcgg ataatgtttt
     3301 ttgttccata acaggcatcc actagtttga aaaggtgggc acctacgtta ccaatactgg
     3361 cttaatggct acatacgacg gtcggtttac gcttaccaga aaactaatag tgctatatgc
     3421 aatttttaat attcagactt tagcctgaca ggcctgacaa aaacgcgccc aacttccctt
     3481 ctatatcact gtctatagta cgtaatctta gcaatggcag accacatttc ttcagtatac
     3541 tgtttttaag ttcatcacgt tcagcctgga cactggtcag atgatagcct ccatccactt
     3601 caataacccc taatggagtc ttcccgacct tatagtaaac aacaaagtca caactggccc
     3661 gattacgcat gaataacagt tcacggtgag taaaatctcc ccgctccagc aacaccaact
     3721 gattcagcgc tatttgtgaa tgaaacatca tactccgata actatcctgc gacaggatat
     3781 cccgcaacag gcaggccaca atctgttctg acttaaagcg tgaatcatct gaattcaatc
     3841 tactattcag tcgttccagt gatttgtcat actcactgta cagcaaatca aatgctgata
     3901 tcaccgggct ctcgaaaatc tctccgtcat cagcgtagta tctgatgtaa cgaatcagtg
     3961 ccgcaatatg tccagcgtgg cgttcaaata catcattacc agtcaccaac gtaaatttgt
     4021 ttcgggccct tgaaacagcg acattcacca gttcaggatt atcaacaaac gcgatattcc
     4081 gactttgctg gctgctgcgt ttcttatcca gcacagtgga aaacacaatt tcatcgcact
     4141 cccgtccctg aaatttatgc accgtcgatt tcacaaaatc agcgggcagc actgtttcag
     4201 ccagattaac ctgtgcatta tatggggcga tatatcccct gctactttcc tcatcccagt
     4261 aatgtccttc cagagattca agctccctga gatttgaaaa attacgggta tggtttcctt
     4321 ttgccgtgat gatcagagat aatgaggctt cacctgaatc aagcgttagc ggtatcagag
     4381 cattatcata aaactgctta ttacaaaatt gaatgatttt aggatgacag cgataatgct
     4441 ctttaagaag agttactggc accatattcc tgaacgacat acaaaccgaa tcaagtagac
     4501 tatatttctc acaattatag tattctgccg gtggagatgg gctgttcggg agtaatacag
     4561 gaacatgtgg aagttgcttt cgatccccta caacgattac attacgggca catcctagtc
     4621 ccagaatgcc cggaacaata tcctgctgtg aggcttcatc aataatcaca taatcaagaa
     4681 gagccccctt accgatagag ttaataatag aatgcgtgct gctaccaata accggaaaac
     4741 gctttataaa acggttaaat tcatccctgt atgtctctgc cgagaatgaa tttcttgttg
     4801 aaatatttct tcgcagatgt tgttttaaat aaagcacaga ccatgatgtc aggcgctcca
     4861 gtaaaatttt aaaatcagat tcctttaata ctttctcata ttcattcaaa gaaatttgtt
     4921 tttcccggat ggatttttca taataagaaa gctgaagaga agtaaatagt gccagccttc
     4981 tctccggaat caccaatggt ttgactttca gtatcctgaa gtttaacaac aaatcaattc
     5041 tgtttctgaa tccaatccgc ctgtcagaaa ggtaatgaat ataagccatc aggtcagttg
     5101 tttttcgtga ggaaagtcgg tatttgtgag aaaaaagctc ctccggtcga agattctgac
     5161 tctgccattg ctgcaggtat ttatactcaa cactcagttc attaatttct gcttttaacg
     5221 aagcaacctg gttgatagca ttcaggtgtt ttttcacttg ttgaagtaca tcatcaatca
     5281 tatttgcaga cggtgagtca ggtaagactt cctcagaacg cgaaatcgac gttgaaaaaa
     5341 actgttgtct gttctccgta cttcccagtc gtgccaccac atatcccagt cgttgtttgt
     5401 ccattttttc ataaacatta ctgacagctg agttattatt ggacaaaatt gccactgttt
     5461 tattctgaat taatatattg gcaacaatat tgagaatagt ctgtgtttta cctgtcccag
     5521 gtggtccttc aataactgaa atctgtgagg aaaatgcccg ttcaaccgcc attaactgac
     5581 tctcattcag gccaaacgga aaaatcaacc cccatggcga atcacgtttt ttactctgtc
     5641 ctgaacagta tgcatgcaag gcggtatccg gatgaggaac tattttcttt atctgtctga
     5701 ggatattctc atcgatattt ttattattac ctcgagcata aaaaacacgt tcgtcagcca
     5761 ccctgcacaa gtaattaaac acggcatgct ctgtaaatgc tgaacactgt tgaaagtcaa
     5821 gtccggtcgt tttcatgatg tagggtcgtt cgctctcacg gtaataaaca gcagcatatt
     5881 tatcaccata gataataacc cgttcagccc gggatgttac agcgttccct ttcctttcga
     5941 ggaaaacatt tcgcaaaatt acagttggcg ttatctgaca tacagaaagc ggacgagtat
     6001 attttttccc gctgcgaaaa tgacaggtca gcataacctc tccgtcatca tttccagccc
     6061 acagagtcca gtcggtaatt ttatgagtct tattttcacc gtcaacacaa atcgtgacca
     6121 tattctcact cagtaattat catttaactt acggaaaatt gttatgaaac tcccgatatt
     6181 tacccggtaa aatacttgtc attgtttgaa gccctcccta aaaaatgacc aggcatttga
     6241 taacccggtg tatttctccc gatatgagct ccggaggatt ttccacaact ccgggtgtgt
     6301 taacatattc ccgccggcca tatatacatt gagttcagtc ataacagtca ataccaagat
     6361 tacaggaacg cgatgctgat acgctggtta ttgaatactg gtagccccta actactaatt
     6421 catcacgata ctctgtactt actgtttttt acaaccattc ccccaggtaa aagagtccat
     6481 ttatcatgcc cctctttaac gcctgccagc ataatttaag aatggatacc gtgggtggaa
     6541 ttaacaatac ctgctcaggc caatgccatg taaccgcctc actgaaaaag ccagatcatc
     6601 taaccaggta cagaccaaca tcgcaaaaat taccatgtag cacgaactgc tgtcatattt
     6661 ctgttcaggt atgccgctat ttcgcgggtt ttcttcacgc cataatgcgt ttcaaggtag
     6721 cgaacatctt cgattgtcca tggtgtgcgc gctcttccgg gggtattttc catttccatg
     6781 ttttatctca gccattatgg ctgcctctcc tgattgcctg tgtgttccat ctgataacgg
     6841 ttaatcaact gattcagcgc cagttctgcc tgtttttgct gctgttcact gccattctgg
     6901 ttgcgggctt caccataacg acgtaactgc tcttctgccg ggatatgccg gttaaaagcc
     6961 tgcatgatgc caaacacctc tgttttcagt tcactgaccg tcatgtattt tccccgctgt
     7021 tcatccagac ggttcaggcg ctcagccagt tgctgtaaac gggccatgcc ttcgttccag
     7081 cccgtcatcg cctcttccgg gagcgctcgg ctccttacgc tcttatgcca gttatccacc
     7141 atttcctgaa catgtggatt gccggggaaa agtcccctca gttgctgcag cagctgcgca
     7201 ctgcgttgca ggttgtatgc cggcggtagt tctgtcagtc gcgttatctg ctgaccggaa
     7261 agggttatcc actctgacgt atgatttaac agtgagggtg ttctgctgag ttgcgccatc
     7321 tggtctgctg aaagcacatc cggtagtggt gccagcagcg ccctgagttg ctgctccgcc
     7381 ggtgatggct gtgtcagaaa gtgccatccc ggaatgacca cgctgacagt cagcaatgcc
     7441 gtacagacac cagccacaaa cggtttcaga aacgtgggcg gggatttctg aagggagggt
     7501 gtgggttctg cttttgtttc agaaacaaca tacaccagac gggagtgcgg ctggggcaga
     7561 tgttcctgta cgtcccgagc atcagaccgg agtgcctgag ccggaagggt taccggcgac
     7621 gatacttcac cctgcacagg ctgattctcc agacgggtga tatatccctt caccatggct
     7681 tccggtaaag ccactttact gctctgttta agttcatgcc gggccagtat gtcactcagc
     7741 gttttcaggc atgtttctgt ctgatacagc aggggcagat tgtcacgctc atccggtggc
     7801 atggcccgga gcgttttttg cagacgactg aacagaccgg taataatttc cagccgggcg
     7861 tgggtattca gcggccacat cactgaccag tgatgccggg tcagcgccac aatcagagcc
     7921 agcccttcat tcagaccact taaccctgct gtatgcatcc gggcgatggt gtaccaggat
     7981 accgtctgta attccacgcc attgtgttca tacagacgca ggcagagcgt ctccacataa
     8041 cgccagtcca catccggacg tgccgggtga gtcagcttgc tgatttcatc gcgcagggca
     8101 gcataatccg gtaatgaccg ggggtcatta cctgttttca gttttacttc agaagtcatc
     8161 ggcagattca gtgttgctgt atcaaatcat aattaatatt cccggaaaga gcgtcagtgt
     8221 aataccggta cccgggaaag gtattccacc gcacggatga ttgccggtgt cacatcccac
     8281 cgccgcttca gaaccgccaa taactcactg agtgttctga tataacgttc agtgaacagt
     8341 cgctgcccgt ccctggttaa atgaaaaccg gactgattac aggtaatggt aatatggcag
     8401 gcatcattac caatatatac ggcttcctgt ggcctgaatc tgacattcca gtgccaggaa
     8461 tacggagcat gattcagata aaaaaggcgc atctggatgt cattaaataa cgggttcatc
     8521 attcagccct cgtttatgtc tgttaagcac acggataagg ggaaccataa atcaccccct
     8581 gaaagtcatc acattatcca caaactgctg ttgcttcaga tatcgcagta acaccctgcc
     8641 ctccggcata aatgttgttc cgtaacgaac cagtcctgcg tctttaagtt ctgcatcaat
     8701 gacataatgc aactcaccag gctgagtctg ctcctgcatg gtcacgtcaa cacgcttaat
     8761 acgtggctca tacttcagca gaacatctga aagcacacac atcagttgat gcgccgttcc
     8821 cggcattccc tgcagaatgc ttgtcatatc cggcagacca taatccggaa gatgtttaag
     8881 gcttccggcc cgggtattca gaatacgctg catgttatcg agcaccgaaa gaataacctg
     8941 ctcttcctca ctgacctggt ttaactccag cccgccagcg aaattgccat agagaatttc
     9001 ataaagggaa ggacgcggca tcactattta tccttcaccg gtaacagcgt cagggtgtta
     9061 ccggatactt caataagacg gggcgtgtcc ggctcaagct catcccggct cagcaccacc
     9121 cgccaggtat ttttcttccg gtcaggctcc agaaacat
//
LOCUS       DAZGCQ010000166         8929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000166 DAZGCQ010000000
VERSION     DAZGCQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.166"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..407
                     /locus_tag="ACHK07_004677"
     CDS             39..407
                     /locus_tag="ACHK07_004677"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO8104906.1"
                     /translation="MSSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESV
                     FLVFLSGILQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQG
                     LGVFITILCVYILNKKYAIE"
     gene            648..833
                     /locus_tag="ACHK07_004678"
     CDS             648..833
                     /locus_tag="ACHK07_004678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104907.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            891..1496
                     /locus_tag="ACHK07_004679"
     CDS             891..1496
                     /locus_tag="ACHK07_004679"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8104908.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIIAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            1493..2410
                     /locus_tag="ACHK07_004680"
     CDS             1493..2410
                     /locus_tag="ACHK07_004680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8104909.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPVDGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            2414..3055
                     /locus_tag="ACHK07_004681"
     CDS             2414..3055
                     /locus_tag="ACHK07_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO8104910.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            3048..4760
                     /locus_tag="ACHK07_004682"
                     /pseudo
     CDS             3048..4760
                     /locus_tag="ACHK07_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            4757..5030
                     /locus_tag="ACHK07_004683"
                     /pseudo
     CDS             4757..5030
                     /locus_tag="ACHK07_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5052..5308)
                     /locus_tag="ACHK07_004684"
                     /pseudo
     CDS             complement(5052..5308)
                     /locus_tag="ACHK07_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5586..6200
                     /locus_tag="ACHK07_004685"
     CDS             5586..6200
                     /locus_tag="ACHK07_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO8104911.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRA
                     KGGGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRY
                     NLDGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
     gene            complement(6421..7905)
                     /locus_tag="ACHK07_004686"
     CDS             complement(6421..7905)
                     /locus_tag="ACHK07_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8104912.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(8214..8618)
                     /locus_tag="ACHK07_004687"
     CDS             complement(8214..8618)
                     /locus_tag="ACHK07_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO8104913.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            complement(8786..>8929)
                     /locus_tag="ACHK07_004688"
     CDS             complement(8786..>8929)
                     /locus_tag="ACHK07_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8104914.1"
                     /translation="DCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASE
                     RMR"
BASE COUNT         2182 a         2099 c         2172 g         2476 t
ORIGIN      
        1 tttagaggaa atgggcgcga accccctttt atgttgttat ctcatcacga cttattgtga
       61 attttgagcc tgttacgttg ttaaccgggc agttgacttt ctcttttatt cttgcttgtt
      121 tttttattgt tctaactggc acatccctgc aactgccaat agaatctgta tttcttgtgt
      181 ttttgtcagg aattttacaa tattttttgg ctttttgttt ctatctttat tccctacgct
      241 ggattcccgt tcacatagca ggagcaatgc tctattttat tcctctggta gcattgctgt
      301 tgtcctggtt cttcctgcag gaagatatat catttattca gggactaggc gtttttataa
      361 ctattttatg cgtatatatt ctcaataaga aatatgccat cgaatgactt tattagggtt
      421 gcgttatcgc ggtagggtac accctgacac cataaccaaa aacgtgatgc cagacgatga
      481 atgacataac cttaatgcga taaagtggta aaaaatgttc atcagagctg atttaaaaga
      541 agtaaagcag tagcccggac cagcgataag gcatcatgca acaaatgtaa cggatatttt
      601 gaaatccttg cctccgcaca tgactgggca taattaaaga aaagcgcatg ctgacacagc
      661 tgcacagagt gctgtaccgt tgtccaggac actatgttta tggttcagcc acacagtcta
      721 ccggcacagg tactggctct ctggcctccc gtaataccag tcagtcgcct ttaccggcgc
      781 agcaggcagg atttccgcgc ccggaactgt attcactttc agtgtgccag tgacagcccc
      841 aataaaaggc cagactcaac cccgtcaaat atgcttatta aggagtgatg atgacccttt
      901 accagataaa gccctcgttt caggctctgt taagaccgct gatgttctgg ctgaacaggc
      961 gcggcgtcac cgctaatcac attacactgt gcgcgatggt gctttccctt atcaccggcg
     1021 tggtgctcat tattcatcct cagcccggat tattcgtcct tctgcccgtg gtgttgtttg
     1081 tacgaatggc gctcaatgcg ctcgatggta tgctggcgcg tgaatgtaat caaagatcgc
     1141 gtctgggggc catcctcaat gagacgggtg atgtaatttc cgacatctcg ctttatctgc
     1201 cctttatgct actcccgcag agcagtatac cgctggtgct gatcgtggtg ttttgtgcgc
     1261 tgatgaccga gttttgcggc gtgctggcgc aaacgattaa cggcgtgcgc agctatgccg
     1321 gtccgctggg caaaagcgac agagcgctgg tgtttggtgc atgggggctg tcactgtcga
     1381 tctggccgca gattatcgcc tggaataatg tcatatgggg tgtcgcaaca ctgttgttgt
     1441 tatggacgat ttttaatcgc tgtcgcagcg cattgcgtga agagggtgtg aaatgacgct
     1501 acttgaaaaa tcactgatcg ttattttttc cctgctgttg attgccaccg taatcaacgg
     1561 gttgctggtg tggctgcgtc caggtaaaga ctggcgcgag ctgacattac gtattcgcac
     1621 ctggtgggtg attatcgtcc tgttttcctt cgcactatta agtcctagct ggctggcact
     1681 gacgttcttc gcgctggtca gctttatggc gctaaaagag tttttgacgc tggtgccttc
     1741 ccgccaatcc gaccgcatgc cgctgttatg gatgtttatt gcaattcccc ttaactactg
     1801 gctgataggt atcggctggt acggcatgtt tgtggtgttt atcccggtct acgtgttcct
     1861 ttttttaccg gcgcgaatgg tgattgctgg ggatacacag ggctttttac gtaccgtttc
     1921 ccagttgcac tggagtctga tgaccaccgt atttgccttc agccatgtcg cgtttttact
     1981 ggtgttaccg gtggatggaa tgcaaaccag cgcgctgttg gtgctgtttt tggtcggcct
     2041 gaccgaattt aacgacatcg cgcaatacct gtggggcaag tcgcttggtc gtataaaagt
     2101 cataccgaag gtcagcccaa acaaaaccct ggagggattg ctgggtgggg tggcaacgac
     2161 ggcgctggcg gcaacagtcc tggggccact gttgactccc ctgagctggc cgatggcact
     2221 gttagccggg gttattatcg gcgttactgg cttttgcggc gacgtggtga tgtcggcaat
     2281 caagcgtgat atcggcgtca aagacagtgg tacgctgcta cccggccatg gaggcatcct
     2341 tgatcggctg gattcgctga tttttaccgc accggtcttc ttccattttg ttcgctattt
     2401 ctgctactaa cctatgatga cacgactact ccgtaccctg tttacgctgt gtatcgtctg
     2461 gccggtcatc tggctgtggc ttggtctgcg tgtccgccac cgcgagcgac tgccgaagca
     2521 cgggccttcg ataatcgtgg caaatcacaa cagccatatg gatgttttcg ccttgctttc
     2581 gctttttccg ctgcgttacc aggcttctgt tcatccggtt gctgccgcgg attattttct
     2641 gcgggataaa cgcatggctt ggtttgcgct gaagatcctt aacatcattc ctatttcaag
     2701 aaagggcggt tataccgatc cgctggcgca atgtgaacag gcgctgcgtg aaggtaaaac
     2761 actgatcctg tttccggaag ggacgcgcgg tgagccaggc gtacttgcgc cattgaaatc
     2821 cggagtgtgg catttgagtc agcgcatgcc ggaggtgccc attatccccg ttgggttgcg
     2881 cggtactgag cgtgtgatgg cgaaaggtaa tcgtatccct ttgccgctgt ttattgatgt
     2941 aaatattggc gatgcgctaa gtacgcatcc ggagaagaaa acgtttatgg acgacctgga
     3001 gcaaagacta acggcgcttt tgcaaccctc gcaaggaaat catccccatg actaattccc
     3061 gcatacccga tgaacggcag tttctgacca gtgataatac agagctgttt tatcgccact
     3121 ggccagcaca agaagccagt tcaacgccaa aagtggtggt cctgtttcat cgtgggcatg
     3181 agcactccgg gcgtttgcaa cacattgttg atgagctggc gatgccggat acagcgttgt
     3241 tacagtccgt cgctcgtgcg ttcggtgcag gatgttgacg agtttgtgcg tttcgccgcc
     3301 gcagatagcc aagctgggat gacggatgtg gtcgtcgtgg cgcaaagcgt cggggcggtt
     3361 ctggcggcaa cctgggcgca tgattatgcc ccggacattc gtggcctggt gttggcatca
     3421 ccggcgttta aggtcaaatt gtatgtgccg tttgctcgcc aggggctgga attgatgtat
     3481 cgcctgcgcg gtcttttctt cgtcaactcg tatgtgaaag gcaagtatct gacgcacgat
     3541 cccgagcggg tggcaagctt caataaggat ccgctgatta cccgaccaat tgctgtgaat
     3601 attctgcttg atctctacaa aacggctgag cgagtcgtca gcgatgccag cgccattacg
     3661 ctacctgtgc agttgctggt ctccggggaa gattacgttg tgcaccgcca gccgcagctt
     3721 gatttttatc agaacctgcg aaatccgcag aaagaactgc atgtactgcc gggattttat
     3781 catgacacct taggcgaaaa agagcggcat ctggcatttg acaggatgcg cagttttatt
     3841 gataccttgt atgcccgtcc cgtgcagcgt tttgattaca gccaggaaga tcgctggagc
     3901 cccagcgccg atacctggcg catactgagc ggtggccctg cgctgtattc gctggacgat
     3961 atctcttacc gctgtttgcg ctacgggatg aaagtgctgg gcacgcagtc cacgggggtg
     4021 cgtctgggat ttgagactgg tttcgattcc ggcagtaccc tagactacat ctatcgcaac
     4081 cagcctcagg gcagcacctc gttgggacgc ctcattgata aaaactacct gaatagcgtg
     4141 ggctggaagg ggatccgcca gcgtaaaatt catctgcaga tgttaattca gcaggctgtt
     4201 gctcggttaa cagagcaggg tagctcggtc cggatcgtgg atattgccgc cgggcatgga
     4261 cgctatgtgc ttgatgccct gcaaggcgaa acggcggtaa gcgagatcct tctgcgcgat
     4321 tacagcgaat taaacgtggc gaaaggtcag gagatgatag cctctcgcgg tatggcgggc
     4381 cgtgcgcgat ttgagcaggg gatgccttta atcgtaatga actggcggca ctggaaccgc
     4441 gtccgacgct ggggatcgtg tccggtctgt atgaactgtt cccggaaaac gggccggtac
     4501 gagattcgct tgccgggctg gctgcagcga ttgaaccggg aggcattctg atctacacct
     4561 gtcagccctg gcaccctcag ttaaaaacca tcgcctggtc actcaccagt cacaaaaatg
     4621 gcaaagcctg ggtaatgcgc gtacgtactc agggcgagat ggacgcgctg gtccgtgacg
     4681 ccggttttga gaaatgcacg caactaattg atgaaatggg catcttcacg gtctctatgg
     4741 cggtacgacg tcaaccatga caacgcggcg tgcggtgatt attcagggaa tcggctggct
     4801 aatggtgctg gccccattct tctttcttac ttatggcatg acgaatcagt ttaccgccgg
     4861 gagagaaaac atcagcagcc tggtgtttga ctgggagcac cggatcccgt taataccgct
     4921 gacgattatt ccgtactgga gtctggattt gctttactgt ttatctgcgt ttcactgaat
     4981 gagcagcggc gtctctggtc tgtcggctgg tgttggcttc ccttattagt acaataatta
     5041 tctatatcca aactggacag ataaaaagtt cccgtgttgt ttttaagagc agcaaaattc
     5101 atggtcggcg aatctcccgg aatacagtca cactgtaacc atgcatcata ctcacgcatt
     5161 tcccggagaa gatcattggc cagggcatgt agtgccgtag ttgctgtttt ttcagtccgc
     5221 agaccgcctt tactgccatc tcttgctcac agctaagctc ccgaatcact ttgctccggg
     5281 gattcttctt tacaagcttc attggcataa tgaatgcact ccttatatac atacttaaac
     5341 agcaagtaaa atatacaaag aacatcccat gaagcaaagg cagagataga ctgtgcgccc
     5401 cgatatattc ttctgatatt ttgctttatt tacttcaaat taatttactt ctgtcagaac
     5461 cacaacaaag tggatacaat atatttttta gaggccgatt attaaacaaa tttatcagat
     5521 gagaatcttt attaatttat aatttgtgat attattaaaa aataaattct gaagcagagg
     5581 tatttatggg aaaagatctg gaagaaaaga aaaatcccgt cattgtggtg aaaaaacgtc
     5641 gctctttttc accgccttcc ttagccgaaa aaaaagatgt tatcgcgcct gcacttactg
     5701 aacaggcgac agaatcagtc tcctccggaa tcagttcacc tgccgtcgaa actcatatcc
     5761 ccgaagcgcc agccaggaag aagaagaaga aaaagaaaag acaacgcttt ccaagaccat
     5821 cccactggac cagtgagttc actcacgatt gtctggagaa ggtgaaagcg ctgtttcctc
     5881 aacttcgtgc caaaggaggc ggttttctgc ctctgaaaac aggcataacc cgtgacatta
     5941 ctacatatct ggcagcacat ccggaaacag cgctgaccct ggacgaatgg aattgtgcag
     6001 tatcctgcat taccagtcgc cggatctacc tgcagagaac ctctgttgca ggaactcccc
     6061 gttataacct ggatggtcaa ccagaggggg aagtatcgga atgtgatgct caaaattcca
     6121 ggcgctggct ggcggcgaaa gagcagaggt ggctgagtat gaaggcccgg caggaaaata
     6181 tttctgagaa atcaaaataa ccgcaggata ttacaggaaa cagactgtag agaagagagc
     6241 gttgtatatt gtgcgtatta tttaagcact cccgagagta atggcttgat tttgcgcgat
     6301 cgcgacattt tttacgtgta tccagtttga cgtttctggt gccgtgatcg cactgaacgc
     6361 attttgctcc caatgcaaaa tatgtccggt atcaggagca aatggcgggc aatttttaaa
     6421 ctattttata tctatttcag catttcctgg acaggatata tttcccattt tcataggggt
     6481 gataagatga cgaacaacgg ccttatctac acataaattg actccggtaa gagctaatgt
     6541 atgcccatca ccatttattg tcagcaacgg gctggaaaat ttctctgcca tcttacgagc
     6601 attaatccat ggtgttgttg gatcgtattt gtgtgctaca aacagtaaac cagagggcag
     6661 aactgtattt ttcagccggg ttttattcag gttgctatgt attggccata attcacaaaa
     6721 atcaggtgaa tcggaacgtc cattgtcaaa gttaatagcc gggaaggcat tcgcaagagc
     6781 gtcttttcgg gattttcgct cttctggggt taattgctcc cccccctgat caacacaaag
     6841 gattaccccc agagcatcac ttgactcttc tgaggctata ggagtactga gcgcagtttc
     6901 aatttcatta ctgacaatcc cctgagagaa ctgacgtatg gcagttgcaa gggttggcca
     6961 tgatgaacgc cataacaaaa ggtctgttgt taataatatg agttcatctg aagatatatt
     7021 ttctcccttg ctgtctaata aaggcttgtg atgcaatttt gataacagct catggaactg
     7081 agttattgcc ttatctctga ctgaagaaag tgggcaactt tttgtacgtg cacaccagga
     7141 tgcaaagcga tcgaacgttt cctgataact ctgtgcttgt ttgagttgcc atgtgaagtt
     7201 gtcccccagg tcatcaatat cgacgacccc atcaagaacg atagatctga tgctggatgg
     7261 gaaacgttct gcatataagg ctgcaatttg tgttccatac gaatatgcca cagctgtcag
     7321 ttgctcgtca cccagggctt gcctgatacg atcaatatca tataccgcct cgttagaacc
     7381 aatatggcga ataacttcag ttccggtatt atggatacag ctgttaattt tatttaatac
     7441 ccgttgcttt tcagttatgt tttcctgaat ccctgtatct gattgctggc agtttattgc
     7501 aggaatggac tggccgactc cccgtggatc aaagccaata atatcccatg attcgcgaag
     7561 atttgttacc ggccagtcaa agttaatata aggattaatg ccaggtaatc ctggtccacc
     7621 acttattata atgatactcc ctttatgcct gctttttgct ggcaattttg tcagcgccag
     7681 ttttacctgc tgtcttttta cagaagaact atctccgcct gtgtctgtat attttaatgg
     7741 aacagacaaa taaccacata gtaagtcagg agacggtttt tcctcaccaa accagttact
     7801 gaatggagtg gtcatacagg attgccactc tatcgactgg gcagatactg ttgcgggcag
     7861 aagtaaggtt aaaactatct tgaaatgagc aattattttt cgcattgtgt ctcttaatat
     7921 cggtataaag ataaagtttg aatatcttaa atccggtgtt acggcaggaa gttacacgtt
     7981 acggatataa ttcaaatgat tacaaagtca gggtatattg tttaactccc cggaactatg
     8041 taagcgtcac cgtcttgtga cgtcttatga tggttattct gtctggtaac aatcggttat
     8101 tttcctggta tcccactgac agcaaaagaa caagactgct gaactataaa cggccatgat
     8161 gaatcatcac gattcggccc cagacttgtc cgtcaggtaa tttttcggtt caatcaccgg
     8221 aagaaattat cgacgtaatc atcccatgaa tgcccctgaa cagaccattc atgattaacc
     8281 ggtggcggga atccacttcg acagtagccg ttcttcccct caaaatcgaa taccccatgt
     8341 ggatatccgt ttatcagcag aacacacttc aggcaatcct cagaccagcc aatcttcaca
     8401 tcagagggga tgtgagcatc tgaaatgtct tcaacgttat atatatgcac agcatccctg
     8461 acagggttac cttcaacgga ctcatcaagt gcatagaaat atccggtatt cccgtcgtct
     8521 tcaaatacag cggccaacat tccatcagga gctaaacttt ttatcgccat agcctcacca
     8581 actatgagtt gagcctgtgc cgtcacaagt attgtcatca aacatgtccc tccgtttcaa
     8641 tttgtctggt aatataaata accccagact catcccggca ggtaaaatca ttctctcaac
     8701 aaaacttttc tttttccgcc tcaggcgagg ccacagagag aataacaatt ctgagagtta
     8761 tctgcgggaa aggctattta tgttactatc tcattctctc actggcgaga taataaacgt
     8821 tactacgttc tcgtttacct accgcaacaa ccgcaataat taacatatca tcaatcacct
     8881 gataaaccag acgaaatccc gatgcacgaa gtttaatttt gtagcagtc
//
LOCUS       DAZGCQ010000167         8812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000167 DAZGCQ010000000
VERSION     DAZGCQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.167"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(356..619)
                     /gene="rpsT"
                     /locus_tag="ACHK07_004689"
     CDS             complement(356..619)
                     /gene="rpsT"
                     /locus_tag="ACHK07_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO8104915.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            722..940
                     /locus_tag="ACHK07_004690"
     CDS             722..940
                     /locus_tag="ACHK07_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO8104916.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            948..1889
                     /gene="ribF"
                     /locus_tag="ACHK07_004691"
     CDS             948..1889
                     /gene="ribF"
                     /locus_tag="ACHK07_004691"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO8104917.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1932..4748
                     /gene="ileS"
                     /locus_tag="ACHK07_004692"
     CDS             1932..4748
                     /gene="ileS"
                     /locus_tag="ACHK07_004692"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO8104918.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4748..5242
                     /gene="lspA"
                     /locus_tag="ACHK07_004693"
     CDS             4748..5242
                     /gene="lspA"
                     /locus_tag="ACHK07_004693"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO8104919.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5330..5779
                     /gene="fkpB"
                     /locus_tag="ACHK07_004694"
     CDS             5330..5779
                     /gene="fkpB"
                     /locus_tag="ACHK07_004694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8104920.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5781..6731
                     /gene="ispH"
                     /locus_tag="ACHK07_004695"
                     /gene_synonym="lytB"
     CDS             5781..6731
                     /gene="ispH"
                     /locus_tag="ACHK07_004695"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO8104921.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6797..7711
                     /gene="rihC"
                     /locus_tag="ACHK07_004696"
     CDS             6797..7711
                     /gene="rihC"
                     /locus_tag="ACHK07_004696"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO8104922.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAVRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVEAQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALAP"
     gene            7878..8699
                     /gene="dapB"
                     /locus_tag="ACHK07_004697"
     CDS             7878..8699
                     /gene="dapB"
                     /locus_tag="ACHK07_004697"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO8104923.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGSIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
BASE COUNT         2019 a         2181 c         2494 g         2118 t
ORIGIN      
        1 taataattta caaggtctaa tgaggagata tttatgaatg gttaaagtat ataggaattt
       61 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
      121 ctttgtaggg tagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      181 ttctacgatt ctttagccac gaagagttca gatagtgcaa cggcatgtct gcaagaacct
      241 gcaacttttg actatctggc aaccggcagt gtgttctctc acgcatcaca aaagcagcag
      301 gcataaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt ggcgattaag
      361 ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg tttttgtgga
      421 tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat gctttctgtg
      481 cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa gtacgcatca
      541 tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg cgcttcttag
      601 ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac aattcaaagg
      661 ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt
      721 aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg cggggtgtga
      781 atacagcttt tccgcgataa aaattgcagc aggcggtcag tttcttcccg tgatttgcgc
      841 catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt taaccttgac
      901 cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg aagctgatac
      961 gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact attggtaatt
     1021 tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca
     1081 agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa ctgtttgcta
     1141 ccgataaagc cccggcacgg ctgacccggc tgcgggaaaa actgcgttac cttgcagagt
     1201 gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg ttttgcggcg ttaaccgcgc
     1261 aaaatttcat cagcgatctt ttggtgaagc atttgcgcgt aaaatttctt gccgtaggtg
     1321 atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag aaagctggca
     1381 tggaatacgg cttcgatatc accagtacgc aaactttttg cgaaggtggt gtgcgtatca
     1441 gcagcaccgc cgtgcgtcag gcgctggctg atgacaatct ggctctggca gaaagtttac
     1501 tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta gggcgcacta
     1561 taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg aaaggggttt
     1621 atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca aacatcggaa
     1681 cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta gatgttgcaa
     1741 tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc
     1801 gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg
     1861 aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg aaacacggaa
     1921 ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca gggttcccga
     1981 tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact gatgatgatc
     2041 tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg catgatggcc
     2101 ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt ctgaaagaca
     2161 ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct ggctgggact
     2221 gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg ggtgagaaat
     2281 tcaccgccgc cgagttccgc gccaagtgcc gtgaatacgc ggcgacccag gttgacggtc
     2341 aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg tacctgacca
     2401 tggacttcaa aactgaagcc aatatcatcc gcgcgctggg caaaatcatc ggcaatggtc
     2461 acctgcacaa aggcgcgaag ccggtgcact ggtgcgtaga ctgccgttct gcactggcag
     2521 aagcggaagt tgagtattac gacaaaactt ctccgtccat tgacgttgct ttccaggcgg
     2581 tcgatcagga tgcgctgaaa gcgaaatttg gcgtaagcaa cgttaacggc ccaatctcgc
     2641 tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac
     2701 ctgatttcga ctatgcgttg gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc
     2761 tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc ggcacggtaa
     2821 aaggtgcgga gcttgagctg ctgcgctttg cccatccgtt tatgggcttc gacgtcccgg
     2881 caatcctcgg cgatcatgtt accctggatg ccggtaccgg tgccgttcac accgcgcctg
     2941 gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc gctaacccgg
     3001 ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc gtgaacgtct
     3061 tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg
     3121 agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg
     3181 cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag tcactgaaag
     3241 agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag tcgatggttg
     3301 ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg atgtcactgt
     3361 tcgtgcacaa agacacggaa gagctgcatc cgcgtaccct cgaactgatg gaagaagtgg
     3421 ctaaacgcgt tgaagttgac ggcatccagg cgtggtggga tctcgatgcg aaagagatcc
     3481 tcggcgacga agctgaccag tatgtgaaag tgccggatac gctggatgta tggtttgact
     3541 ccggatctac ccactcttct gttgttgatg tgcgtccgga atttgccggt cacgcagcgg
     3601 acatgtatct ggaaggttca gaccagcacc gtggttggtt catgtcttct ttgatgatct
     3661 ccaccgcgat gaagggcaaa gcgccgtatc gtcaggtact gacccacggc tttaccgtgg
     3721 atggtcaggg ccgcaagatg tctaaatcta tcggcaacac cgtttcgccg caggatgtga
     3781 tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaactgac tacaccggtg
     3841 aaatggccgt ttctgacgag atcctgaaac gtgcagccga cagctatcgt cgtatccgta
     3901 acaccgcgcg cttcctgctg gcaaacctga acggttttga tccggcaaaa gatatggtga
     3961 aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg
     4021 aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag cgtctgatgc
     4081 gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac cgtcagtaca
     4141 ccgccaaagc ggatagcgtg gcgcgtcgta gctgccagac tgcgctgtat cacatcgctg
     4201 aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa gtgtggggct
     4261 atctgccggg cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa ggcctgtttg
     4321 gtctggcaga cagtgaagca atgaacgatg cgttctggga cgagctgttg aaagtgcgtg
     4381 gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt ggctcgctgg
     4441 aagcggcagt aaccttgtat gcagaaccgg aactggcggc gaaactgacc gcgctgggcg
     4501 atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat aacgacgcac
     4561 ctgctgatgc tcagcagagc gaagtactca aaggactgaa agtcgcgttg agtaaagccg
     4621 aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag gtggcggaac
     4681 acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt
     4741 ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc ttgtggtagt
     4801 cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg ctctggggga
     4861 tacggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt
     4921 tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg
     4981 tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga agctaaacaa
     5041 tatcgcttac gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca
     5101 cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa
     5161 ccttgccgat actgccatct gtgtcggtgc ggcactgatt gtgctggaag gttttttgcc
     5221 ttctaaagcg aaaaaacaat aataaaccct gccggataag gcctacagat tgctgcgaaa
     5281 tcgtaggccg catccggcaa aaatcctcaa aatataagag caaacctgca tgtctgaatc
     5341 tgtacagagc aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac
     5401 cgccgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggcg atgcttctct
     5461 ttctgaaggg ctggagcaac acctgctggg gctgaaagtg ggcgataaaa ccaccttctc
     5521 gctggagccc gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg
     5581 ccgtgaattt atggatgcag gcgagccaga aattggcgca attatgcttt ttaccgcaat
     5641 ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga
     5701 tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga
     5761 tccggcactg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg
     5821 gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat
     5881 atgtccgtca cgaagtggtg cataaccgct acgtggtcga tagcttgcgc gagcgtgggg
     5941 ctatctttat tgagcagatt agcgaagtgc cggacggcgc gatcctgatt ttctccgcac
     6001 acggtgtttc tcaggcggtt cgtaacgaag cgaaaagccg cgatttgacg gtgtttgatg
     6061 ccacctgtcc gctggtgacc aaagtgcata tggaagttgc ccgcgccagc cgtcgtggcg
     6121 aagaatctat tctcatcggc cacgccgggc acccggaagt ggaagggacg atgggtcagt
     6181 acagcaaccc ggaaggggga atgtatctgg tcgaatcgcc agacgatgtg tggaaactga
     6241 cggtcaaaaa cgaagagaaa ctctccttta tgacccaaac cacgctgtcg gtggatgaca
     6301 cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag
     6361 atgacatctg ctacgccacg actaaccgac aggaagcggt acgcgccctg gcagagcagg
     6421 cggaagtggt gctggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggaac
     6481 tggcccagcg catgggcaaa cgcgcgtttt tgattgacga tgcgaaagat atccaggaag
     6541 agtgggttaa agaggtaaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc
     6601 tggtgcagaa tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc attccgctgg
     6661 aaggccgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg
     6721 aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat
     6781 tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga
     6841 tgccgtcgcc attgccgccg cgatttttgc ccccgaactc gacctgcaac tgatgaccac
     6901 cgtcgcgggt aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac tgctgcattt
     6961 ctggaatgcg gagattccgc tcgcccaggg agccgctgtg ccgctggtac gcgcaccgcg
     7021 tgatgcagca tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa
     7081 ccgaaagcca ctcgggatac cggcgtttct ggcgattcgg gatgccctga tgcgtgcacc
     7141 agagcccgtt accctggtgg ccatcggccc gttaaccaat attgcgctgt tactttcaca
     7201 atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt ctgccgttcg
     7261 cggcaactgc acaccaaacg ccgagtttaa tatcgctgcc gatccagaag cggctgcctg
     7321 tgtcttccgt agcggcatcg aaattgtcat gtgcggtctg gatgtcacca atcaggcaat
     7381 attaacccct gactatctcg ctactttacc ggagttgaat cgcaccggaa aaatgctcca
     7441 cgccctgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa tgcacgatct
     7501 ctgcgccatc gcctggctgg tgcgcccgga cttattcacc ctcaagccgt gttttgtcgc
     7561 ggtagaagct caaggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct
     7621 gggcaaacca gccaatgtac aggtggcatt ggatctgaat gtaaaaggct tccagcagtg
     7681 ggtggctgag gtgctggctc tggcgccgta acctgtcaca tgttattggc atgcagtcat
     7741 tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca
     7801 gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt
     7861 aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg
     7921 ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg
     7981 cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg
     8041 cagggaaaac aggcgttacc gtgcaaagca gccttgatgc gataaaagat gattttgatg
     8101 tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc
     8161 atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcgattc
     8221 gtgacgccgc cgccgatatt gcgattgtat ttgccgccaa ttttagcgtt ggcgttaacg
     8281 tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa
     8341 ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg
     8401 gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg
     8461 aaggccatac cggtgaacgt gtgcctggta gcattggttt tgccaccgtg cgtgcaggtg
     8521 acatcgttgg cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc
     8581 ataaggcgtc cagccgcatg acatttgcta acggtgcggt aagatcggct ttgtggttga
     8641 gtggtaagga aagtggtctt tttgatatgc gagatgtgct tgatctccat aatttgtaac
     8701 cacaaagtgt ttatggtgat gcaaaagtaa cgcatttaat ttattgaata taaagggctt
     8761 taatttttag cccttttatt tttggtgtta tgcttttaaa ttttatgcaa ac
//
LOCUS       DAZGCQ010000168         8807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000168 DAZGCQ010000000
VERSION     DAZGCQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.168"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..851)
                     /gene="mlaA"
                     /locus_tag="ACHK07_004698"
     CDS             complement(96..851)
                     /gene="mlaA"
                     /locus_tag="ACHK07_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO8104924.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1145..2077
                     /gene="yfdC"
                     /locus_tag="ACHK07_004699"
     CDS             1145..2077
                     /gene="yfdC"
                     /locus_tag="ACHK07_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO8104925.1"
                     /translation="MDNDKIDQHSDEIEVESEEKESGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2153..2227
                     /locus_tag="ACHK07_004700"
     tRNA            2153..2227
                     /locus_tag="ACHK07_004700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2186..2188,aa:Arg,seq:cct)
     gene            2389..3546
                     /gene="intS"
                     /locus_tag="ACHK07_004701"
     CDS             2389..3546
                     /gene="intS"
                     /locus_tag="ACHK07_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO8104926.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IISLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            3919..4872
                     /gene="ompT"
                     /locus_tag="ACHK07_004702"
     CDS             3919..4872
                     /gene="ompT"
                     /locus_tag="ACHK07_004702"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO8104927.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            5059..6543
                     /locus_tag="ACHK07_004703"
     CDS             5059..6543
                     /locus_tag="ACHK07_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8104928.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKPENIYCPGNSEAEIQ"
     gene            complement(6727..6939)
                     /locus_tag="ACHK07_004704"
                     /pseudo
     CDS             complement(6727..6939)
                     /locus_tag="ACHK07_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            7089..7757
                     /locus_tag="ACHK07_004705"
     CDS             7089..7757
                     /locus_tag="ACHK07_004705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8104929.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(7702..7839)
                     /locus_tag="ACHK07_004706"
     CDS             complement(7702..7839)
                     /locus_tag="ACHK07_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO8104930.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(7812..8396)
                     /locus_tag="ACHK07_004707"
     CDS             complement(7812..8396)
                     /locus_tag="ACHK07_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8104931.1"
                     /translation="MAFRMSEQPRTIKIYNLLSGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(8396..>8807)
                     /locus_tag="ACHK07_004708"
     CDS             complement(8396..>8807)
                     /locus_tag="ACHK07_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104932.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         2583 a         2030 c         1928 g         2266 t
ORIGIN      
        1 ccgggtcatt gtcggtttat ctccttttac ttgtgataat tgagggataa caacaaaaaa
       61 ggtgagtatt gcgactcacc ttttttattt gtttcttatt cagaatcgat atcttttaaa
      121 tcatcctgaa tcgcttgcgc gttcggattt tcctgcggtt tgagttcgcc gccattagcg
      181 atgaaatcat gacgctggaa gtacgcttcg cgcaccataa tataaggatc ggacgactga
      241 cgcagcagac cgtcggaatc caacaactgc gcacgagttt ctatcccttc aagcgtccat
      301 ttacccacag acatcggcca ggtcagccag gatagaaccg ggtaaagacc atccgccata
      361 tcaccaccgt catcacgcag cgtgaagcta ccgtagaacg gtaactggac gtaaggccca
      421 taacccacgc cataatgacc aagcgtacta ccgaagcggt gaggttcagt ccgttgcagt
      481 ttcgggttcg ccatccctgc aacatcaata aaaccaccca tccccaaaat ggtgttcagg
      541 aaaaagcggg taaagtggac catcccctga taagggtcgc cctgcaagaa gtagttcacc
      601 atcaccgcgg gttcttcaag gttgccggta aagttgctca aaccgttacg cgccggttgc
      661 ggaacataat cacgccaggc gacagcgacc ggtcgaacaa tatacgggtc tagtacgttg
      721 aagttgaagt tgtacatggt gcggttgaac ccttctaacg ggtcagaacg cccttgctga
      781 tctgtaccgg aactcgcaca ccccaccaga agcgtagttc ccagagcaag cgccgacagg
      841 cgaagcttca taaatgtctc cctgtttttt tatggcttat gcagtttgcc atccatgacg
      901 gaacgatacc gtatccgcct gtttaggtgt gggcgattgt aacagcacgt caactgatgt
      961 ccagacgccc tgatttgctg atttgatcat agcctggtaa tcgccgccct gtaggctact
     1021 tgattctata gaaacagaaa aaggcaaacg cggccttttc tacattttca gagtaactcc
     1081 cgccagttgc gagcgaaaaa gccgctacgc tttagctata cgtgctaatt caagagaaga
     1141 gaccatggac aacgacaaaa ttgatcaaca cagcgacgaa attgaagttg agagcgaaga
     1201 aaaagagagt ggcaaaaaaa tagaaataga tgaagatcga ctcccctccc gggcgatggc
     1261 aattcatgag catatccgcc aggatggtga aaaagagctg gaacgcgacg caatggcact
     1321 actgtggtca gccattgcgg cgggtctgtc gatgggcgct tcattactgg caaaagggat
     1381 atttcatgtc gaactggaag gtgtgccggg cagcttcttg ctggagaatc taggttatac
     1441 ctttggtttt attatcgtca ttatggcccg ccagcaatta tttaccgaaa acaccgtgac
     1501 tgctgtacta cccgtcatgc aaaaaccgac aatgagcaac gttggcttac ttatgcggtt
     1561 atggggcgtc gtgctgctgg gtaatattct cgggacaggt attgcggcgt gggcatttga
     1621 atatatgcca atcttcaatg aagaaactcg cgatgcattt gtcaaaatcg gcatggatgt
     1681 gatgaagaac acccccagcg aaatgtttgc caacgcgatc atttccggct ggctgatcgc
     1741 cactatggta tggatgtttc ctgcagcggg tgcggcaaag attgtggtga ttatattgat
     1801 gacctggctt attgccctgg gtgacaccac ccacatcgtt gtcggttctg ttgaaatcct
     1861 ctatctggtg tttaacggca cgctgcactg gagcgatttc atctggccct tcgcactacc
     1921 tactttagcg gggaacatct gcggcggcac ctttatcttc gcgttaatga gtcatgcaca
     1981 gatccgtaac gacatgagca acaagcgcaa agcagaagca cgccaaaaag cagaacgtgc
     2041 ggaaaacatt aagaaaaatg ataaaaaccc ggcataaatg gcgagggttt aagcaatcgc
     2101 acggcagcgt acttaccccg cagtccatta gcgggtatac tcattccgca ttgtcctctt
     2161 agttaaatgg atataacgag cccctcctaa gggctaattg caggttcgat tcctgcaggg
     2221 gacaccattt atcagttcgc tcccatccgt accagtccgc aaaatcccct gaatatcaag
     2281 ccttccgtag attcacagtt cgtaatggtt cgcgtcagat cgttgacagc cgcactccat
     2341 gacgggtaaa aagtggataa aataatttta cccaccggat ttttacccat gctcaccgtt
     2401 aagcagattg aagcagcaaa gccgaaagaa aaaccatacc gcctactcga tggtaatggc
     2461 ctgtaccttt atgtccctgt gtcagggaaa aaggtatggc agcttcgcta caagattgac
     2521 ggtaaggaga aaatcctgac cgtaggaaaa tatccgctaa tgactttgca ggaggcaaga
     2581 gataaagcat ggaccgcgag gaaagacatc tcggttggca tcgatccggt aaaagcgaaa
     2641 aaggcttcgt ctaacaacaa ttcctttagt gcgatttaca aggaatggta cgagcacaag
     2701 aagcaagtct ggtcagtagg ctatgcaact gaacttgcaa aaatgtttga tgacgacatt
     2761 ttacctatca tcggcggcct tgaaattcag gatattgagc cgatgcaact gctggaagta
     2821 atccgcagat ttgaagatcg cggtgcaatg gagcgagcca acaaagcccg cagaagatgc
     2881 ggcgaggttt tccgttacgc tattgtcact ggtagggcta aatataaccc ggcacctgac
     2941 cttgctgacg ccatgaaggg ataccgcaag aagaacttcc cgtttctccc agcagaccag
     3001 atccctgcat tcaacaaagc actggcaaca ttttcaggaa gcatcatatc gctcattgcg
     3061 accaaagttt tacgctacac agccctaaga acgaaagagc ttcgctccat gcaatggaag
     3121 aacgtcgatt ttgaaaacag gattatcact atcgacgcca gtgtgatgaa gggccgcaag
     3181 attcatgttg ttcctatgtc agaccaggtg gttgaacttc tcactacgct aagctccatc
     3241 actaaaccag tatcagagtt tgtttttgcc gggcgcaacg ataagaagaa gccaatctgc
     3301 gagaacgcgg tattgcttgt gatcaaacaa atcggctatg agggtctgga aagcggtcac
     3361 ggattcaggc atgaattcag cacaattatg aacgagcacg aatggcctgc tgacgctatt
     3421 gaagtgcaac tggcacatgc caacggcgga tctgtgcgtg ggatttacaa ccatgctcag
     3481 tatctcgata aacgcagaga aatgatgcaa tggtgggcgg actggattga tgaaaaggtg
     3541 gagtgatcca ccttaaccac tatcgaagag cacaaagcct tgcaatccag tacaaagcta
     3601 tgtgtgtctc agttttgtcc ctcttttttg tactaaaaaa catagcaatt gaggataaac
     3661 ctcatgctat tttcgcttat atgcctttaa aggcatggcg cttaaataga taaaaacacc
     3721 acaaaagcat aaaaaaccac acagtaaaac cgcaacataa aacaataaca gataattaaa
     3781 ccaaaaacag atagcgcatt gtgataatca ttcaatgcta aacaaaatat aaacagtgga
     3841 gcaatatgtg attgactcat taagttagat ataaaaaata catattcaat cattaaaacg
     3901 attgaatgga gaacttttat gcgggcgaaa cttctgggaa tagttctgac aacccctatt
     3961 gcgatcagct cttttgcttc taccgagact atatcgttta ctcctgacaa cataaatgcg
     4021 gacattagtc ttggaactct gagcggaaaa acaaaagagc gcgtttatct agctgaagaa
     4081 ggaggccgaa aagtcagtca actcgactgg aaattcaata acgctgcaat tattaaaggt
     4141 gcaattaatt gggatttgat gccccagata tctattgggg ctgcgggctg gactactctc
     4201 ggcagccgag gtggcaatat ggtcgatcag gactggatgg attccagtaa ccccggaacc
     4261 tggacagatg aaagtaggca ccctgataca caactcaatt atgccaacga atttgatctg
     4321 aatatcaaag gctggctcct caacgaaccc aattaccgcc tgggactcat ggccggatat
     4381 caggaaagcc gttatagctt tacagccaga ggtggttcct atatctacag ttctgaggag
     4441 ggattcagag atgatatcgg ctccttcccg aatggagaaa gagcaatcgg ctacaaacaa
     4501 cgttttaaaa tgccctacat tggcttgact ggaagttatc gttatgaaga ttttgagcta
     4561 ggtggcacat ttaaatacag cggctgggtg gaagcatctg ataacgatga gcactatgac
     4621 ccaggaaaaa gaatcactta tcgcagtaaa gtcaaagacc aaaattacta ttctgttgca
     4681 gtcaatgcag gttattacgt aacacctaac gcaaaggttt atgttgaagg cgcatggaat
     4741 cgggttacga ataaaaaagg taatacttca ctttatgatc acaatgataa cacttcagac
     4801 tacagcaaaa atggtgcagg catagaaaac tataacttca tcactactgc tggtcttaag
     4861 tacacctttt aacgaagtta accagatttt ctccccggtg gtattgtata accccgggga
     4921 atttgtttac agttacaagt aggcaatatc cactaatttt gtaagcattt aacctatgtc
     4981 ataacgtgta atctcttacc gcaacacaag acctcaatat attcaaatct tatctttatt
     5041 ccgatattca gagacacaat gcgaaaaata attactcatt tcaaagttgt tttaacgtta
     5101 cttctaccag taaccgtatc tgcccagcag atacagtggc aatcctgtat ggccagtcaa
     5161 ttcaaccact ggtttggtga ggaaaaaccg tctcctgact tactatgtgg ttatttgtct
     5221 gttccattaa aatatacaga cacaggcgga gatgcttctt atgaaaaaaa atcacaagtc
     5281 aaactagcgt tgacaaaatt gccggcaaaa agcaagcata aaggaagtat cctgataata
     5341 agtggtggtc ccgggttacc aggcataaat ccttatatta actttgactg gccagtcaca
     5401 aatcttcgtg agtcatggga tattattgga tttgatcctc gaggcgtcgg acagtccact
     5461 ccgacaataa actgccggca atcagataca gagactcagg aaaacataac cgaaaagcaa
     5521 caagtattaa ataaaattaa tgcctgtatc cataataccg gagccgaagt cattcgccat
     5581 atcggctcta acgaggctgt atacgatatt gatcgtatta ggcaagcctt gggggataaa
     5641 caactgacag ccgtggcgta ttcgtatgga actcaaattg cagccttata tgcagaacgt
     5701 tttccctaca acgtaagatc tatcgttctt gatggagtcg tcgatatcga tgacctggag
     5761 gacaacttca catggcaact caaacaggca cagagttatc aggaaacgtt tgatcgcttt
     5821 gcatcctggt gtgcgcgtac aaaaagttgc ccgctttctt cagacagaga taaggcaata
     5881 actcagttcc atgagctatt atcaaaatta catcacaaac ctttattaga cagtaaggga
     5941 gaaaatatat cttcagatga actcatatca ttaacaacag accttctgct atggcgttca
     6001 tcatggccaa cccttgcaac tgccatacgc cagttctctc aggggattgt cagtaatgaa
     6061 attgaaactg cgctcagtgc tccgatagcc tcagaagagt caagcgatgc ttcgggggta
     6121 atcctctgtg tagatcaggg ggatgagcaa ttaacaccag aagagcgaaa atcccgaaaa
     6181 gacgctcttg cgaatgcctt cccggctatt aactttgaca atggacgttc cgattcacct
     6241 gatttttgtg aattatggcc aatacatagc gacctgaaca aaactcgcct gaaaaatact
     6301 gttctgccct ctggtttact gtttgtagca cacaaatacg acccaacaac gccctggatt
     6361 aatgcccgta agatggcaga gaaattttcc agcccgttac taacaataaa tggtgatggg
     6421 catacattag ctctcaccgg agttaattta tgtgtagata aagcagttgt acatcacctg
     6481 atcactccac aaaaaccaga aaatatatac tgcccaggaa attctgaagc agaaatacaa
     6541 taatttcaaa atattccgct atttgctccc aatgcaaaac atatattgca ttgggagcat
     6601 attcatattt ttgttattct tgtatgaaat cgtctcatgt acacggtcct tttttcgccg
     6661 aaaactgata ttactttaat caaaagttga cgtgccagat tcgaagtaaa tttataaaat
     6721 atcaaatcaa tatacatttt gcaccttcga gatagtaatg gctgttactg taacatgagt
     6781 tacaaacagc ttcataccca gagctactga tgtatcaact cgattcaaca atacccgata
     6841 tttattccat gcctccagca acgatctttc ttcctccgtt gcgatttcca gatctacagc
     6901 atcctgcagt ggcgcaatat actcactgaa ttcctggatg tagaactgtg tggtgacggt
     6961 cttccagcca ttcggctcct gctgtatcga agcataccat gctatttcaa tatcgctatg
     7021 ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt cacaaataaa
     7081 actataacat ggtgaaatta atgaaaaaaa acacagatga tggggctaaa atttacacac
     7141 cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg cttgcatggg
     7201 gatgtcctac aaaggaacac cttcttccac actttctgga acatttaggt aacaaccatc
     7261 tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt agtctgatat
     7321 ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct ggggaatcaa
     7381 aaattaaaca taaaattaac catgatgttt ttgaacctta tcccgcggcg ttacatggtc
     7441 aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat atatctacaa
     7501 aaagctgtgt aatacgcaat gcggcgcagg ccttaactga cgatggaact ctatacggag
     7561 ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg atgcgcattt
     7621 acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta acacatatac
     7681 tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta atgttttccg
     7741 cttcagggaa aaaatagcat ccaaccgcag cacgttcttg cttaagacgt gctgcggcat
     7801 aatcccaatg attactccct gacagggttc gtaggccact caatatcagg tgcagttgat
     7861 gtatcaacac ggttcagcaa cacccgatac tttttccagg cttccagcaa caaggtttct
     7921 ttctccgttg cgatctccag atccacagca tcctgaagcg gcgctatatg ctcactggct
     7981 acctgcatca ggctgttttt tgtttcttcc gcctcccgga tccggaacag tttttctgct
     8041 tccgtatcct tcacccaggc tgtgccgttc cacttctgaa actccccttc cggggataac
     8101 caggtgacat tttccggtaa tgagccgagt tcagaaataa ataacgcgtc gccggaagcc
     8161 acgtcataaa ccgttttacc ccgatgatct tcaacgagat gccacgatga ctcatcactg
     8221 ttgaaaacag ccacgaaacc agccggaata tctggcggtg caatatcggt actgtttgct
     8281 ggcagacctg tatgaggcgg aatatatgca tcaccttcac caataaattc attagttccg
     8341 gacagcagat tataaatttt tatggtccgt ggttgttcac tcattctgaa tgccattatg
     8401 caagcctcac aatatagtta aatgcgatgt ttttgacggt gttttccgcg ttaccagcag
     8461 cgttaacggt gatggtgtgt ccatgtgagc caatcgcaac cgagtgcgta tgagcaccaa
     8521 taccgacagt atgtgcgtga gcacctgcag atgcagctgt gccgctgaca ctatgagtgt
     8581 gcgctcctgc agcacttgta ttcacactgg ccgcagacac taccttatgt actgacccat
     8641 tttgtgacca ctggctgact gctgatgcgc ctgtgacact atgagtatgg tttccggcac
     8701 ttgcggctgt accgctcaca ctgtgcgtat gcgccccgat gttattcgtg gatttagtgc
     8761 cgtaatcaaa cgacgatgtg gttttcgtcc ccaaatccgt actggat
//
LOCUS       DAZGCQ010000169         8716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000169 DAZGCQ010000000
VERSION     DAZGCQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.169"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            521..1864
                     /locus_tag="ACHK07_004709"
     CDS             521..1864
                     /locus_tag="ACHK07_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104933.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(2013..3521)
                     /gene="putP"
                     /locus_tag="ACHK07_004710"
     CDS             complement(2013..3521)
                     /gene="putP"
                     /locus_tag="ACHK07_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO8104934.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3680..3889)
                     /locus_tag="ACHK07_004711"
     CDS             complement(3680..3889)
                     /locus_tag="ACHK07_004711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104935.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3944..7906
                     /gene="putA"
                     /locus_tag="ACHK07_004712"
     CDS             3944..7906
                     /gene="putA"
                     /locus_tag="ACHK07_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8104936.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQTVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7946..8584)
                     /gene="rutR"
                     /locus_tag="ACHK07_004713"
     CDS             complement(7946..8584)
                     /gene="rutR"
                     /locus_tag="ACHK07_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO8104937.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         2227 a         2350 c         2223 g         1916 t
ORIGIN      
        1 cacgattttt ctactatgac attatcacaa acccactagc atgaatacag aaagagaagt
       61 aataagatga gacgccactg ctttcagatg aaccctgtaa ttagggtcga gaatatagtt
      121 tatccattcc cctatcgata aattaaaaaa tttgcgtatt aatttttctc tgactgaagg
      181 ctaacgtcga attaccgaca agattcacga gtgattgtaa ctacgtactt tatgaaacac
      241 aggaagaaat tactatttac cgcgatagtt caaggcgtaa aaaaaataat aatattcatg
      301 aaactcatcc cgatggcagc attgtaaatt cgccaataac tggcattcct cctgcttttt
      361 acgggtgttt ccattaacac cagaatgagc ggattgttta ctacatcaag aacgcatccg
      421 aagtgtacat cctggtgcgc cgactgaatc gcccggagcc atgcggatgg ctacgactta
      481 atacgaatca ttaaccctgt aaggatacgg agagcatgac atgagaccaa tatccaacct
      541 caatacgagc gcagcaacct atattccacc gcaacaactc ccgagtagtt gtctggaaac
      601 tttgacgctt ctggtgcaga tgaataatta tatcaaaagg gatgcggact acagcacggg
      661 catggctgtt actccactgg ttcctgaaga ggttcacttg ctggctgttg cgatgacaac
      721 cgaactacat cgacatcagt tccaaccggt ggtaagtgca aatgatctgc aacttcctga
      781 accgttcacc ttcgatgttg ccggtttcac catcaccttc acgaagacgc aagagcatga
      841 caacacgggc aatctcatga aaattgcagt cagcaagagc ggtgtgtgca ccagtacaaa
      901 tattactctt gagctgtttc actccattgt cacaaccctg atgtcacgca gtcaatatgg
      961 aacctttgat ctctggtccg tacggccaat actcacggat gaaagtcaaa acagggtgca
     1021 tgaagcagct cgctactccc cggcccagca atacggaaga gaagaaacac actttaccaa
     1081 caatggcatg cgagaatttg gaaatatgtc tccgctggcg tggcgtaacg atgtggaatt
     1141 acagcaatcg tgtaatacaa gcaacatccc accaaatgct gcaaatgacg acataaacaa
     1201 caccaggcag accatcgagg accagccaga ggcggacgag ccacagcaat atgttaaatt
     1261 gactgtagac gacatgcgaa aatgggcggc tatggaccag caggcgcgaa acgctctaca
     1321 gggagtttca ggatggtgta cgcgcaatca ttttaatatc aaaaatgcca gaaattacct
     1381 gaccgatcac ggcctgaact acgcgggaca ggtaaaggta aacaggccgc atgaatatgc
     1441 caaattcacc cttgaacata tccgccagtg ggcagcctta aacaagcatg tgcgaaaacc
     1501 ggttggttat ttagaaaagt ggtgtaagga aagaaacctc gcgccaacta ctgccaggaa
     1561 ttacctgaag aacgacgggc ttaccgccct gggtgagttg aagctgacag ggccgcagaa
     1621 atgggtcacg ttcaccttcg gagacatcgt acaatgggcg aatatgaccc aggaggagcg
     1681 taacagcgca gggggagccg aaaagtggag taaaaaacgc ggcttccagt ggtctactgc
     1741 cagatcctac ctgaaatcga gcggagtgac caagcaaggt gcgagaaaac tggcgtggtt
     1801 aaaaaacagc gggaatatgt cgaatccatt ctatctcgcc caaccaacca gccgcaggac
     1861 ataagctgaa ggtaccgata gcggcaattt tgttcatcag tgccgggtgg gcaaatagca
     1921 attgcccccg aacactccct gccaggcaac ttataccgca tggcagggaa cattcgggtt
     1981 aaaaagcaaa agataaataa ttactcttca gcttagcctt cctgcaaccg tgacggcggt
     2041 gccgaatgat agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct
     2101 ttacccagca aactaaacac tacaatccca atactgccga agataaagcc cggaataatt
     2161 tcgtacagtc ccagccagcc gaactgtttc cagacgataa ccgtcagcgc cccgatgatc
     2221 atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc
     2281 ggaccaaacg ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt
     2341 tccgggtttg ccgccagcgc aatggccacc agcgccacca ccagcaccat cacacgccct
     2401 acccacacca gctctttctg gctggcatgt ttacgcagaa acgctttata tagatcttcg
     2461 gtaatcgcac tggaacatac cagcagctgg caacttaagg ttgacattac cgccgccaga
     2521 attgccgaca gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata
     2581 aacacgcgtt cagcgttctg gtttaccgca cctgctaccg ccgggtgctc gttaaagtaa
     2641 gcgataccaa agaagccgac agccaccgcc cctgccaggc agaggatcat ccaggtcata
     2701 ctgatacgac gcgcatggac gatgctgtgg tgagaatccg ccgccataaa acgcgccagg
     2761 atgtgcggct gcccgaagta acccagcccc catcccatca gcgagataat ggcaacaaag
     2821 ttcagccctt tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagagag
     2881 tcgccaaagc caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc
     2941 atcaggctgg cctgtacagt atcagtccag ctcaccgcga ggaaaccgcc aataaaggtg
     3001 taaaggatcg tcgccgcagc tcccgcccac agagccgttt cgtagctcat gccaaaggta
     3061 ctttcaaaca gacgcgcgcc tgccacaatg cccgaagcac aataaatggt gaagaacagc
     3121 aaaataacca gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa gcgtccggtg
     3181 aaataatccg gcaatgttaa ggcgttattg ttatattcgg tatgcacacg caaccgcccg
     3241 gccaccagct tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccaactttcg
     3301 gaaatcccgg aaagaaaaac cgcgcccggc aaccccatta acagccagcc gctcatatcc
     3361 gatgcacccg ccgataatgc cgtcacgaat ggcccaaggc tacgtccgcc cagaatatag
     3421 tcgtcaaagt ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata
     3481 tagacacaaa atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt
     3541 attatcggca atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt
     3601 atgttttacc agggcgaccg tatcctgccg gaagcgctag ttattcacaa tcgatttaac
     3661 acaccattta cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc
     3721 tcacattttt tgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgag
     3781 ttattttttt aatccctgtc atatcgattt cttttattaa catttcattc atttttaagc
     3841 ttgctacgca agtcacattt aacatggttg cacaaagttg caacatcatg gatatttcac
     3901 gataacgtta agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat
     3961 gggggttaag ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga
     4021 tcgcacgcca cactggttaa ttaagcaggc gattttttct tatctcgaac aactggaaaa
     4081 cagcgatact ctgccagagc tacctgcgct gctttctggc gctgccaatg agagcgatga
     4141 agcaccgact ccggcagagg aaccacacca gccattcctc gactttgccg agcaaatttt
     4201 gccccagtcg gtttcccgcg ccgcgatcac cgcggcctat cgccgcccgg aaaccgaagc
     4261 ggtttctatg ctgctggaac aagcccgcct gccgcagcca gttgctgaac aggcacacaa
     4321 actggcgtat caactggccg ataaactgcg taatcaaaaa aatgccagtg gccgcgcagg
     4381 tatggtccag gggttattgc aggagttttc gctgtcatcg caggaaggcg tggcgctgat
     4441 gtgtctggca gaagcgttgt tgcgtattcc cgacaaagcc acccgcgacg cgttaattcg
     4501 cgacaaaatc agcaacggta actggcagtc acacattggt cgcagcccgt ccctgtttgt
     4561 taatgccgcc acctgggggt tgttgtttac tggcaaactg gtttccaccc ataacgaagc
     4621 cagcctctcc cgctcgctga accgcattat cggtaaaagt ggtgaaccgc tgatccgcaa
     4681 aggtgtggat atggcgatgc gcctgatggg cgagcagttc gtcacgggcg aaaccatcgc
     4741 ggaagcgtta gccaatgccc gcaagctgga agagaaaggt ttccgttact cttacgatat
     4801 gcttggcgaa gctgcactaa ctgccgcaga tgcacaagcg tatatggttt cctatcagca
     4861 ggcgattcac gccattggta aagcgtctaa cggtcgtggc atctatgaag gtccgggcat
     4921 ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat
     4981 ggaagagctt tacccacgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg
     5041 tatcaacatt gacgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa
     5101 actctgtttc gagccggaac tggcaggctg gaacggcatc ggctttgtta ttcaggctta
     5161 tcaaaaacgc tgcccactgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg
     5221 ccgtctgatg attcgcctgg taaaaggcgc gtactgggat agtgaaatca agcgtgcaca
     5281 gatggacggc cttgaaggtt atccggttta tacccgcaag gtgtataccg acgtttctta
     5341 tctcgcctgt gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcgac
     5401 gcacaacgcc catacgctgg cggcgattta tcaactggca ggtcaaaact actatccggg
     5461 tcagtacgag ttccagtgcc tgcatggtat gggcgagcca ctgtatgagc aggtcaccgg
     5521 aaaagttgcc gacggcaaac ttaaccgtcc gtgtcgtatt tatgccccgg ttggcacaca
     5581 tgaaacactg ctggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt
     5641 tgttaaccgt attgccgaca cctctttgcc attggatgaa ctggtcgccg atccggtcac
     5701 tgctgtagaa aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc
     5761 cctgccgcgc gatctttatg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa
     5821 cgaacaccgt ctggcctcgc tctcttctgc cctgctcaat agtgcactgc aaaaatggca
     5881 ggccttgcca atgctggaac aaacggtagc ggctggtgag atgtcgcccg ttattaaccc
     5941 tgcggaaccg aaagatattg tgggctatgt gcgtgaagcc acgccgcgtg aagtagaact
     6001 ggcgctggaa agtgcggtta ataacgcgcc aatctggttt gccacgcctc cggctgaacg
     6061 cgcggcgatt ttgcatcgcg ctgccgtgct gatggaaagc cagatgcagc aactgattgg
     6121 tattctggtg cgtgaggccg gaaaaacctt cagtaacgcc attgccgaag tgcgtgaagc
     6181 ggtcgatttt ctccactact acgccggaca ggtgcgggat gatttcgcta acgaaaccca
     6241 tcgtccatta gggcctgtgg tatgtatcag cccgtggaac ttcccgctgg cgattttcac
     6301 cgggcagatt gctgccgcac tggcggcagg taacagcgtg ctggcaaaac cggcagaaca
     6361 aacgccgctg attgccgcgc aagggatcgc cattttgctg gaagcgggtg taccgccagg
     6421 cgtggtgcag ttgctgccag gtcaggggga aaccgtgggc gcgcaactga cgggtgatga
     6481 tcgcgtgcgc ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa
     6541 tatcgccagc cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg
     6601 catgaacgcg atgattgtcg attcttcagc actgaccgaa caagtagtaa tagatgtgct
     6661 ggcctcggcg ttcgacagtg cgggtcagcg ttgttcggca ctgcgcgtgc tgtgcctgca
     6721 agatgagatt gccgaccaca cgttgaaaat gctgcgcggc gcaatggccg aatgtcggat
     6781 gggtaatccg ggtcgcctga ccactgatat cggtccggtg attgatagcg aagcgaaagc
     6841 caatatcgag cgtcatattc agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt
     6901 acgggaaaac aacgaagatg cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct
     6961 gatcgaactg gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt
     7021 ggtgcgttac aaccgtaacc agctgccgga gctgatcgag cagattaacg cttccggtta
     7081 tggtctgacg cttggcgtcc acacgcgcat tgatgaaacc atcgcccagg tcactggctc
     7141 ggcccatgtc ggtaacctgt acgttaaccg taatatggtg ggcgcagtgg tgggtgtgca
     7201 gccattcggc ggcgaaggat tgtccggcac cgggccaaaa gcaggcggtc cgctctatct
     7261 ctaccgtcta ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga
     7321 tgcagagtat ccggtcgatg cgcagttgaa agccgcattg actcagccgc taaatgcact
     7381 gcgggaatgg gcagcaaatc gcccagaatt gcaggcgtta tgtacgcaat atggcgagct
     7441 ggcgcaggca ggaacacaac gattgctgcc agggccgacg ggtgagcgca acacctggac
     7501 gctgctgccg cgtgagcgcg tattgtgtat cgcggatgat gaacaggatg cgctgactca
     7561 gctcgccgcc gtgctggcgg tgggcagcca ggtgctgtgg ccagatgacg cgctgcatcg
     7621 tcagttagtg aaggcattgc catcgactgt cagcgagcgt attcaactgg cgaaagcgga
     7681 aaatattacc gctcaaccgt ttgatgcggt gatcttccac ggtgattcgg atcagcttcg
     7741 cgcattgtgt gaagcagttg ccgcacggga tggcgcaatt gtttcggtgc agggttttgc
     7801 ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc gagcgttcgc tgagtgtgaa
     7861 taccgctgcc gctggcggta acgccagctt aatgacgatc ggttaaacag gtagccggag
     7921 gatgttacac cttcctccgg catcattagc gtggtcgaat cccctcaata ataatccgct
     7981 gcacgttttc aaccgtttga ttgaaaaata cctcatcgcg caacgttgcg cctgtcaccg
     8041 cctccacctg aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc ataaaaatca
     8101 aatgctgcgg atcaatcggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg
     8161 atttctcatc aattaatgcc ttcaaatcgc ccgtcagttc atccattaac agcggcgcgc
     8221 ctgccagcat ctccatacag aacaggcgcg aagcctgcgg ataatcgcgt gagacttcca
     8281 gcttcagacg gatgtactct ttgatcgccg ccagcggggc gaaatcttca cgaaacgctt
     8341 ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt
     8401 ttgacggaaa gtaatacagc agattggttt ttgaaacacc cgccaactct gcgatctgct
     8461 ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg
     8521 cttttttctt cgcactgact gcgcgcgaac gtttacccgt tgttttcact gcaccttgcg
     8581 tcatgcgctc tcccctcttg tagattggca acaggatagc aaaacccccg cccggcctcg
     8641 accttctgca ctctcatcgc gcacggatgc atgttttatg tgcaactgtt ttgaccgttt
     8701 agtccacttt ttacca
//
LOCUS       DAZGCQ010000170         8670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000170 DAZGCQ010000000
VERSION     DAZGCQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.170"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1931
                     /gene="espL4"
                     /locus_tag="ACHK07_004714"
     CDS             <1..1931
                     /gene="espL4"
                     /locus_tag="ACHK07_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO8104938.1"
                     /translation="INKNENTTLTQTIAPIFEEYLMEILPQRSDALDKQKLNLNSDRK
                     EKEFPRVKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVNYKPHQDDYSSAEKFLTH
                     FNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHAL
                     TARLRIKNTTEGYTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKW
                     SNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQ
                     LQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLM
                     HILEAKDKNGFSGLFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTS
                     SHVLYHVMANGDADMLKIVLDALPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQN
                     GHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIF
                     NALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLF
                     GFTAEDIMGFWQHKAPQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIA
                     EKNYMEALLKKASPHTVR"
     gene            complement(1900..3636)
                     /gene="aceK"
                     /locus_tag="ACHK07_004715"
     CDS             complement(1900..3636)
                     /gene="aceK"
                     /locus_tag="ACHK07_004715"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO8104939.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLSDL
                     PLRLPWQNKSRDINYIIRHLTETLGPENFSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDYGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRTLQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3820..5124)
                     /gene="aceA"
                     /locus_tag="ACHK07_004716"
     CDS             complement(3820..5124)
                     /gene="aceA"
                     /locus_tag="ACHK07_004716"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO8104940.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5154..6755)
                     /gene="aceB"
                     /locus_tag="ACHK07_004717"
     CDS             complement(5154..6755)
                     /gene="aceB"
                     /locus_tag="ACHK07_004717"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO8104941.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7024..7953)
                     /gene="metA"
                     /locus_tag="ACHK07_004718"
     CDS             complement(7024..7953)
                     /gene="metA"
                     /locus_tag="ACHK07_004718"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO8104942.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8110..8553
                     /locus_tag="ACHK07_004719"
     CDS             8110..8553
                     /locus_tag="ACHK07_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8104943.1"
                     /translation="MVISIRRSQHEESEKLIAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TSNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2232 a         2111 c         2121 g         2206 t
ORIGIN      
        1 taattaataa aaatgaaaac acgacactca ctcaaaccat tgctccaata tttgaagaat
       61 acctaatgga aattttacct caacgctcag acgctcttga taaacaaaaa ttaaatctaa
      121 actcagatag aaaagaaaaa gaattcccaa gagttaaact taatggtcaa tgttattttc
      181 cggggcgccc acaaaaccgt atagtatgcc gacacattgc tgcacaatat attaatgata
      241 tttatcagaa tgttaattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc
      301 tcacgcactt caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg
      361 aggggcgttg tgttgctgcc tgcggtgatt tcgggctagt tatgaaggca tattttgaca
      421 agatggaatc aaatggcatc agtgttatgg ctgccatatt attggtagat aaccatgctt
      481 tgacggcccg actaagaata aagaacacaa ctgaaggata tacccattac gtggtttcgg
      541 tttatgatcc aaatgtaact aacgataaaa taagaatcat gagcgaaagt aaagaggata
      601 ttaaacacta ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa
      661 atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat
      721 tgatgttaaa aggatctgtg gatgaaataa cccctccatt atcacctgca acgatgaatt
      781 tgctaatggc aattggtcag aatcaccaac ttacgcaact gatgattcaa ctccagaaaa
      841 tgccagaact acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt
      901 tatatttggc tataaattat ggtaatgcgg atatcgttga gactattttt aattcattgt
      961 cagagccagg atatgaagga ttactctcga aaaaaaatct catgcatatt ctggaggcaa
     1021 aagataaaaa tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg
     1081 taacctcgat tctgaacgcc ttacctaaac tggccgcaac acatcattta gataacgaac
     1141 aagtgtataa attcctgagt gccaaaaata gtacgtccag ccatgtttta taccatgtta
     1201 tggcgaatgg tgatgccgac atgctgaaaa ttgttttgga cgcgttacct ttgttaattc
     1261 gcacatgtca tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg
     1321 gttgcccagg actatacctg gcgatgcaaa atggacatag cgatatcgtg aaagttattc
     1381 ttgaagcatt gcccagcctg gcccaggaaa ttaacatttc agcttccgat attgtagatc
     1441 ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac
     1501 atatgaacgt tattaatact atttttaacg cattacccac tctgtttaac acgtttaaat
     1561 tcgataaaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
     1621 tgttttcagc gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat
     1681 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatgggtttt tggcaacaca
     1741 aagcgccaca aaaatattct gcctttgagt tggcttgtga attgggtcac cgggttattg
     1801 ctgaattaat ctttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
     1861 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
     1921 ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat gtccttcacg
     1981 aatgcggttt tgtagtgtgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
     2041 aaagagtggc ccaatgcggg ggtcggcgca tagccagtgg cggaactctt ccgggaaaac
     2101 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
     2161 cggcgggatg tcgcggaagt tcacttccgt catgtagcaa atttcatcgt aatcataaaa
     2221 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat
     2281 gttagcggcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cacgcaactg
     2341 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct caatataaag
     2401 atggcgaatc acaatttgtt cgccgagatc ggtgattttt tccgctgctt cctggagcag
     2461 taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
     2521 ggtgtccgcc attcggccca cgcgatcatg ctctttcacc agttgatagc aggcacgaac
     2581 gtgagcggca gacatctctt tctgcggcgc gaacctgtct ttgatgactt tgaatacccg
     2641 atcaaagccc ggtagcgtaa acaccaacat caccatgcca cgaatacccg gcgcttcaat
     2701 gaactgctca ttacagccct gtagataaac gagatattcg cggtagctct cggttttggc
     2761 atgcttctgg cagccgatag ccatatacaa ttcagcggtg gttttacctg gcagaatttc
     2821 ccgtaaccac tcgaccagcg ctgcgggcag cggcgcataa accataaaat aagaacgcgc
     2881 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     2941 gccgtagtcc gtctggtgga tcggcagcaa aaatggcaat gtgccggaag gcgtgatcag
     3001 tttgcctacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     3061 acttttggag aagttttctg gccccaacgt ttccgtcaga tggcgaataa tataattgat
     3121 atcacggctt ttattctgcc agggcaggcg cagcggcagg tcactgagga ctcgcatcag
     3181 cagcgcttcc cagccatgct cggggtagaa gtctttcgcc agtgggcgcg gaatggtacg
     3241 aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg
     3301 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     3361 atccggcaac agccgggtgt aatgctcttt gacgcgcagt aaaaattccg cgtccgtgct
     3421 ttggccgttg gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     3481 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     3541 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     3601 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     3661 aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat
     3721 gataagacgc gcaagcgtcg catcaggcat cgtgcactga ttgccggatg cggcgtaaat
     3781 gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt
     3841 ggagccggtc agcgcggtga ctgaagacgt gccgccctga ataatggtcg tcactttatc
     3901 gaagtaacct gtacccactt cctgctggtg agatacgaag gtgtagccat ctttcgcggc
     3961 ggcgaattcc ggttgctgca ctttctcaac gtagtgcttc ataccctcgc cctgggcata
     4021 ggcgtttgcc aggtcgaaca tgttgaacca catgctgtgg atccccgcca gggtgatgaa
     4081 ctggaacttg tagcccatat ctgacagctg ctgctggaag ctggcaatag ttttgtcgtc
     4141 gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata
     4201 tttcgcgtgg atggcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
     4261 acaccatacc aggtcagcat atggtgcata cgccaggcca cggctgatcg cttgctcaat
     4321 gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc
     4381 atacgggtcg caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag
     4441 cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc
     4501 ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag
     4561 ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca taagttcaaa
     4621 ggcattcagg acaccgccaa aaccggcttc cgcgtcggca acgatcggca ggaagtaatc
     4681 gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat tggatctgat ccgcacggcg
     4741 gaaagtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg
     4801 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
     4861 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag
     4921 gctgttgatg tagccttttt tcgactcgcc atgcagcaga cgccacattt tcgccgcgcc
     4981 cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc
     5041 gctgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
     5101 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
     5161 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
     5221 tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc gcccagttcg
     5281 ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt
     5341 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt
     5401 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc
     5461 catgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt
     5521 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc
     5581 acttcaagct gatttttacg ggagccgaga atgtcgttaa ataccgccat tgccgtatcc
     5641 gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgattta
     5701 tccgctttta ctttgttgag cacttggtta ttgcgctctt catctttgct cggaataaac
     5761 gccgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt
     5821 gagtaagcat tcaggaatgg tttatccatc gtcactgcct gtctgtctgg caggacgcga
     5881 tcgggatagt ttttcaacgt tttgatataa ctgaagatgt aatcccaacg accgcagttc
     5941 agaccaacaa tatggtcacg cagtgcgtga aggatttcgt ccatctggaa cacggcgggc
     6001 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
     6061 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
     6121 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
     6181 gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc
     6241 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctggtaaatt
     6301 ttgcctgctt cattcgtgta actgatggtg ccgttaaccg catcgcgcag gttaatttgc
     6361 ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
     6421 ttcacattgg cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct
     6481 acgcggcggt cttctaagtc cgcaggaatc ccgcgaattt tccaatcagc atcgcgaatg
     6541 gaagctgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga
     6601 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
     6661 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa
     6721 gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
     6781 aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac
     6841 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac
     6901 ttattgataa caatatgaat aaaatttatt cacctactga ggtgtgtttc ggaaataaaa
     6961 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
     7021 agattaatcc agcgttggat tcatgtgccg tagatcgtat ggcgtgatct ggtagacgta
     7081 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggtgt
     7141 attttgcgga tcattgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc
     7201 cacatcgcgg aaaaattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
     7261 aaaggcaatg cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc
     7321 cagaatttcc agatcggtat aatcacgaat caacgctgcc ggaaagtcag catagcgcga
     7381 atgtggtgcc aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
     7441 atgatgctcg taaacgccag agagtttgtc ggtgcgagtt tgcttaggta tgccgtagag
     7501 gatattgagc gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt
     7561 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
     7621 gcccagcggc gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa
     7681 gttacagtag aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
     7741 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
     7801 ttcagtttca atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc
     7861 ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
     7921 ggcgggtagc tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt
     7981 atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc
     8041 atgttgaagg tgagaaagtt tcaaggaaaa gactgcgtta gaatataaga atgactaaaa
     8101 ggagagaaaa tggttattag tattcgccgc tcgcagcatg aagaaagtga aaaactgatt
     8161 gcgatttggt gtcgttctgt cgatgccact cacgattttt tatcagcaga gtatcggacc
     8221 gagctggagg acctagttcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
     8281 gaacgggatc agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
     8341 atcgatcctg atgtgcgcgg ctgcggcgtg ggccggatgc tggtgaagca tgcgctctca
     8401 atggccccgg aactgacaag caacgttaat gagcaaaatg agcaggcggt tgggttctat
     8461 aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat
     8521 ccgttgctga atctggcgta tgtgggggaa taagctgccc cctatcgtgc tatcgtgcct
     8581 gcgtaagctg gataagacgc gccagcgtcg catccggcat ttttttgcac caacggcaaa
     8641 aaggccatcc gtcaggatgg cctttcgctt
//
LOCUS       DAZGCQ010000171         8655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000171 DAZGCQ010000000
VERSION     DAZGCQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.171"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..861
                     /locus_tag="ACHK07_004720"
     CDS             79..861
                     /locus_tag="ACHK07_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8104944.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNKYLKHLTHEGVEVEFMTIDITKPDAACTIINRCLERFGQLDILVNNAV
                     ICNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKVINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
     gene            complement(1007..1696)
                     /locus_tag="ACHK07_004721"
     CDS             complement(1007..1696)
                     /locus_tag="ACHK07_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8104945.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(1693..3027)
                     /locus_tag="ACHK07_004722"
     CDS             complement(1693..3027)
                     /locus_tag="ACHK07_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104946.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNTDGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAYPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            complement(3043..5565)
                     /locus_tag="ACHK07_004723"
     CDS             complement(3043..5565)
                     /locus_tag="ACHK07_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8104947.1"
                     /translation="MNKSVVSISAAMLVLLYQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVATTNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            complement(5618..6319)
                     /locus_tag="ACHK07_004724"
     CDS             complement(5618..6319)
                     /locus_tag="ACHK07_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8104948.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVVIRAAELLSNGKKVPLANSVMIAPQSTTEWTLRSGISVARGALIHLV
                     TVNDYGVNVTSEHAL"
     gene            complement(6405..6965)
                     /locus_tag="ACHK07_004725"
     CDS             complement(6405..6965)
                     /locus_tag="ACHK07_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8104949.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLLPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            complement(7008..7631)
                     /locus_tag="ACHK07_004726"
     CDS             complement(7008..7631)
                     /locus_tag="ACHK07_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104950.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFLMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLITLIKSNDKDQQVRQVTLTRYEWDIYYLYFKIDNKRILHTLLKKDV
                     KYISHYKISVFNKFEIACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
BASE COUNT         2315 a         1902 c         2015 g         2423 t
ORIGIN      
        1 tttagttttt tattgaaatc taaaaattac ttaaattaca ccttaaatat tacttactca
       61 taaggagcaa tgtgtgatat gagcttttct attgaagatt tttcattgga ttttttttct
      121 ttacataaca aggtggccat cgtaactggg gccgcgggtg aactggggag aggcttatgc
      181 tctgctctgg ccaaagcagg agctaatctg ttgttggtag acatcaaaga gcctgataat
      241 aaatatttaa agcatttaac tcatgaaggc gttgaagttg agtttatgac aatagacata
      301 acaaaacctg atgcagcatg caccattatt aataggtgtc tggagcgttt cggtcagtta
      361 gatattctgg tcaataacgc ggtgatttgt aacattaatc gtcctatcga tttcaatcga
      421 aacgactggg atccgatgat taatctcaac ctgaatgctg cattcgatat gagccaggcc
      481 gcattgaata tatttgtccc gcaaagaaaa gggaaagtta ttaatatgtg ttccgtattg
      541 tcatttcatg ggggacgttg gtcgccaggg tatgcggcga ctaaacatgc tttagcgggg
      601 ctgaccaaag cgtatgccga tgattttgcg gaatataata ttcagattaa tggtatcgcc
      661 ccaggatatt atgtgagtga aatgaccgct attatttata ataaccctaa aataaaagag
      721 ttaataaaag ggcgtatacc ggctcaacgc tggggaaggg cacaggatct tatgggcgca
      781 atggtgttcc tcgccagtgc cgcttcagat tatgtcaatg gacaactatt agtcattgat
      841 ggtggatatt caattcgtta acgatttttg gctgcgttga ggtgttttga ctggaaaatg
      901 gagtggagag attttgtcat ttctccactt ccggtacttc aggtgggtat agtggttatt
      961 cgttagcgtt gaaccatcgc gatgagttta cataccactc agagcattat tgcgctaatt
     1021 gttgttttat ctgtgcacca ttatcatcga gatatgtcgc cgcaaccaca ccactaacgt
     1081 tattacttgc taaccagtac cgtcgttgac tgaagggttt tatcatcata tcgctttctt
     1141 gctgcacctt tgtagcccca atgctgatgg aggtgaaaga gatattcagt ggtgttgggt
     1201 tattaagctc aatatagcta ctgctgccgt cgttacccag atgccaactt attttacgca
     1261 tagcttcttg gggcgtcata gttacagatt ttggcctgta aaagatttta agttgcgtat
     1321 tcattgccag cgttaaatgt gtgggatctt gatggggagt ttctggcggc atttcataaa
     1381 gatttaacca gaatgcagat tcgcgatcct ggggaagtgg gtcatgatta tagataatcc
     1441 gcaacccctg ggttcgtctc gcagccaggc ggaaaaccgg tggcagcacc acaaaggggg
     1501 gagaagcagc ttctggggaa ccttcaccat tatctaccca actttgcacc agtaccggat
     1561 agtcattggt attgaccagc atcagtgtct tttcccgatc gccatcgcga taaataatcc
     1621 gtgaagaagc cggcatgatg ccggcttcca ctgactgtac gaacaagacg gttaagagca
     1681 gcaaaacgga tttcattgca ttttcaccaa tatatacgcc gttgcccgca cttttcccgc
     1741 actgactgtc tgatttggca gtttcttcaa ccgggcgata aaagagtaac tatagctgga
     1801 gtatccgggg tgagtcgcac cttctaatgt tgccccttca aatacaggat accaccccgc
     1861 tgcatttcct ccgggggtta acttcgcgat gcccggttga ccaaccagcg tcatcgccgt
     1921 atcggggtaa gcactgtttg agatgtagat cccaacgccc tttgccatct ctgctgcgtc
     1981 ataattatca gagactaggg cgctgacgcc gccgttgcta ttgataatcc ccagtttttg
     2041 cgccgctgta tatgcacctt cagacacctg gattcctaat gcagtctgag tatcgctaat
     2101 ccctgattga accgagttac tacactcgac gcggacatta aaattaccgg tactttccat
     2161 gcccgcattc agttgtgctt ctgcaattgt cggcagcaat actaacggcg tggcgctgcg
     2221 ggcaacgcag gtggcattgt tgtagagtcg attggccgaa cgcattccgt aaccaaaacc
     2281 attatcagca ccccaaaaat catagtgatc cgcactgtcc tcgcccactt catcatgagc
     2341 aaatggaata ccactggtac cggaaagctg aatataggca ttaggctgag tgcagttata
     2401 aggtgtgccg gagggtttag cataccccac accgtcgcca tcagtattgg tattaccgca
     2461 gtaactggtt gcgccagcgg tatcaggcag cgtactaata cggtaaagct cagcatgaag
     2521 aggagggata tcctgtaagc ggatctggat tttagttccc tgagtggcat aactagtgat
     2581 gggcactttt ttccagtaac gaccaatcgt cacgcccgcc atagtctgct tgagaccgac
     2641 aaaagcaaac caggtggcat agacatcact cagaccatca ggcccgcctg cattgtgaaa
     2701 gccacccacg cggtcgtcgc cattggttgc gaccagaaaa tagatgtttg gcaaatctgt
     2761 tgcgtcacat tcccacagta cggagcttcc tgacgcgcca cctgatgtgt agttagtgga
     2821 aggtacgact gttgtcgcga gtagtgagcc tactggcgca aaataggtgt cggtcaggtt
     2881 aatttttccg aactcaatga gtgccgatct tccgtcctcc gtttgtccga cacgtgtatt
     2941 agaacaagtg gcccaggcgg atgattgtat tagtgccacc aatatgatgg agctaatcag
     3001 aatgtgtaaa aaagtcgatt tagacatcgg gttttctctg tatcaagact gacaggtttc
     3061 attcaggcgg atgatagggc tattgctgtc tttcccgctg atatcaaagg gcaactggca
     3121 gctatcgtta gcgccttcac cccagcgaac tgacaaatgg ccttttgtct gttctgtgcg
     3181 gaggtaaatt tgccccccct gaccggctat accgacaaca gtattattct catccagcac
     3241 atcggctccc attggcagtt ccgaaccatc tgccatgcgg gatttaatca gcagcgcttt
     3301 accaggacgg gtacggaaga ctactttcac cgccgcaccc gcaaccggtg ctacctgttt
     3361 ttcactgtcg accagttcgg catcgccatc cattccttgt ggatcgagag atatacggtt
     3421 gtagcgatag ggcgttactg ccggaacaag cgcatagcca ctgtcattaa tttccagctg
     3481 actggagtta tagacttttg caccttctgc gccttttgct tcgaccaggg cgaacgtttc
     3541 acccagataa ggtccgaaag tgacgccgcc gccatggacg gccatcgcgc cttgtacgtt
     3601 tccggacgcc tgccagtaat cctggccctt cgatgcgttc aatccgacgg tagtttgtga
     3661 aaaacgtttt tgcatgtttc cgctaagcgt cgtctgctta tattgttgat cgcgcatgac
     3721 gttcaggctg tagttggtgg tctgtgcttc atcaagcatt ccggttagcg agctttgtaa
     3781 ttgcgagcta ccgtcagttg aatgggtcca gctgttgcta agacttggta cacgaggtcc
     3841 attgccgcca agtgggaatg agaatgaaag ggacgttacc gtctgcatat catcagaatt
     3901 gtctttatag ccgcccattc tttggcgtcc gacggaaagg ttcatactga tgccgtggct
     3961 aaagctattg ctgtaaccta actgaagctg tgtatcacgg ctcttgccgc cacggtagtt
     4021 ctgtgttgaa cctgacacaa acaggttgcc gtaattcgca aggctctgac ttaacgtaag
     4081 atcgaagcgc gactgttgac gccactggct ggagtcccat gactgtttat tactggcagc
     4141 atgacgctct cccagcacat cagccagatc acgatagccg ctggtagagt atcgataacc
     4201 tgccagggag acggtggttg aagtaggctg gaaagtttta ctccaggtta attgcgacat
     4261 ccaaccactc tgtgcttcgc tttcgggaac acgcgcatgg gaccaagtga ggtttgcccc
     4321 aaatgccccc agcgaactgc catagactcc gccaagcatc agcgcctggt agccatcagc
     4381 aatacgcgag ccactgttaa atgttagcgt attagtcagc ccgtgctgcc aggtgaagtc
     4441 accaaacatc gagtcatcac cgctatcctg cgttttacct acttcaacgt tataacggga
     4501 agttcctgga cgcatcgatt ctggtacggc tgaaaagggg acactgaaac gactgactgc
     4561 gccattagct tccgtaacgg tgacatccag atcgccgctg tagctggtgg ggtataggtc
     4621 gtttatttcg aaagggcctg gagccacggt agtctgataa atttcatgac cgttttgcat
     4681 taccgagact ctggcgtttg ttgtagccac gccgcgaata gtcggcgcat agccgcgcat
     4741 ggagtccggc agcatacgtt catcggtcgc gagactaacg ccgtgataac tgagtccaga
     4801 gaaaaatctt ccgctggtga taagttgccc catcattaat tgactattta tggcgggcag
     4861 cgggcgttgc aaatagctac gaatattgtt ccactgattc cctttgtcgt tatcccaggt
     4921 catgttggat aactgacgat attgccaggc accaaggtta atgccgccat taaatgatgc
     4981 ccatagcgaa cgctggctat gagcattctg ccctgaatag gcaacgttgt aatagttggc
     5041 aatataattc atgaacgcca gcgatgctcc ggtatccagc tcgctagggg gaacatagcc
     5101 gcgagggcga ctaatcaatt gactctgagg caccgacaag tccaggcgca attgtgacaa
     5161 ctgtgtctgg ctggtgatgc cgggagcgag ttccttagca aacgtacaga caggatcttt
     5221 ttccccctta tcttcccctg ttttgaggcc tatcgcgtcc gcgacttcag gcgtcaggca
     5281 gggcagaacg cgaccatcag cggcttcttt aaacgtgaca ttccaactgc ctgacaactt
     5341 attgttggtg tagatatcca ttttataatt gcccggtgca acagactcac gtgttgtcag
     5401 ttttgctaat gacgactgac taaatctgct gccgcggaag agttgcgggt caaaggtgta
     5461 gttgtcttca tctgacggtg ttgcgggtga gatttcgctc cccatgaccg gttggtaaag
     5521 taaaaccaac attgccgcag aaattgacac aacggattta ttcatctcga cgtactcgtg
     5581 cgtgaaaaag gataccgctc aaaaaaggag aaagaggtta taaggcatgc tcagacgtaa
     5641 catttacgcc atagtcgttg acggtcacta aatgaatcag cgcaccgcga gcgacactga
     5701 tgccagagcg tagtgtccat tcagttgtgc tttgaggagc aatcatgacg gaattcgcga
     5761 ggggaacttt tttaccatta ctaagcagtt cagcggcgcg aatcacgacg taatagcccg
     5821 tgggattcgt cacttcaatg cggttctgtt ttacctggta agtcaggttt ttgggtgcgt
     5881 cactggattt accgacaatc gagctgggac gatagaaaat tttgactcga ttagtcaggg
     5941 ccaggatgag ctggttctga cttttatcat tcttattcag ataaggtagt tgactgaaac
     6001 taaaccagaa aacagactcg cgatcctgcg gtaaattatt accagtgtag agcaggcgta
     6061 acgcctgacc tgcggcagct tccatgcgaa atgtctgcgg ggttggcata aacggtacat
     6121 tcttgtcaga accacgttca tcctcaacat ggatattaat gatatagggc tgatcgctgg
     6181 tatttcgcaa ctggatggtt ttttcctttg cgtcaccagg gaaaataata cgggttccgg
     6241 tcattaccac gctggcaaac gcgctgctgc tcatgctcat ggaaaataca agtaccagta
     6301 aatatgcaaa aatgttcatt cttatctttt ccatgtccat gaaaaatcac tattaattgt
     6361 gcgaagaaat actggctggc atttactgcc agccagtaga ttaattagag gtagctcagg
     6421 gtgtattctg cgacagcggt aattttaccg gcagtggcag ctgcgctatc gataacgaag
     6481 taacgcgcac cgaagtcgta gctggcttca gtatcgccaa ctttcagggt caaacccggt
     6541 acgttagtcg cgccagccag agttactggg ttaccagaag tgctggaatc cattaactga
     6601 atgccaaagc ctttcgccgc atcgctgctg gtatcacggt ttcccataac acccgtgcta
     6661 gcgtcaacgt cgtagcccag gaaagtggtg ttgattgcct gatctgcacc agtcggagcc
     6721 tggcagttag acacagaaac tgtaaacggc gtctggcctg cgctctggcc atcagctaaa
     6781 gttgcaccga agtcagccat ggctacggtc ggcagcaata ctactgaatt ggtttgaccg
     6841 ttgatattaa cggaacaggt ctgatcggtt acttcaccct gaaaagtgac tgtcggtgca
     6901 gccatggtag aaaaaggtaa tgcgatagct gccagcagag ctgctttttt gaaagtagaa
     6961 aacatattaa tatccttgaa atttagagtt aatatagaac gagataatta atgcaattga
     7021 tacaatctga cgtttttaaa accggtaaat ctccttaact gtataagtgc cttgaaaatg
     7081 taaaagccat cactatcgca agcaatttca aacttgttga aaactgatat tttatagtga
     7141 ctaatgtact tgacatcctt ttttagcagg gtgtgtaata ttcttttgtt atctatctta
     7201 aaatataggt aatagatatc ccactcatac cttgtcagag tcacctgcct gacttgctgg
     7261 tctttatcat tacttttaat caacgtaatg agtttattca attttatata tcggctaatg
     7321 aatgtttgct ctgaaagtga aaagaagata tttctgtttt tcatctttct tagtgaaaca
     7381 atcattaaaa acctggctaa atctggcagt tttgcaagta tacttacatc atccactgcg
     7441 acaagaagta cgtttttggt aaagcggtta tcaaatctaa gtgtacttag gtcattatta
     7501 caagcatttt gagaacaaaa aaaacactca ataaaaaaac gataatggtg accaagtttt
     7561 gcacttaatc cctgaaggta atatagatca tccgaaatga taattaccct tgttagtaag
     7621 ttcttgttca ttgcactaac tcaattgctt gcagcaaaac agcccatctt cttggagtaa
     7681 aaattttgaa agtgaaaaaa tttcttacta aagatgtaac aatgggcttt ccatatgcga
     7741 cataacaact gcagtaacac aactaatctt tatgtgttct ttatatagag agtaacagcg
     7801 tgatatctgt atctctaaaa gaatagcgat gccctgcaat cgatgagcac aatatatgac
     7861 attcatcaca tgtataacaa ttgaatttac attcatactg gtgttaatcg gtaatatagt
     7921 aatttaattc ttaccgaaag atgatgggca gggttttatt ctgtgtaatt ttgtttttgc
     7981 gatgatttaa ggttgtaaat gctgcataat gattgtacct attacttttt atgtcaaaaa
     8041 agccgttttt ataagaataa aacaacgata tttaagagtg ctgacattgg gaaaaagggt
     8101 taatacagtc atttaagatt atttcagctt ttcctgtggt gggaaatggg cgcgaatgta
     8161 gtgctttctg taaaaaacat caactcttaa cgaataaaaa ataaagtata atcattgagt
     8221 taggttgttt tgctgcagaa tgataacaac attatgaaaa tcattacaat ctttaatttt
     8281 gaataacatt ttcatgaagc ccagagtacg ttaagggatg atgtataggg gtaattgcta
     8341 ttagatggga atgaaacctt gtgaaccatt ttgctcttct ttctaccttt caacggtaag
     8401 ccaaacataa tgtggctgct taagctaatt gtcgcaaagc caaagagctc tctctatata
     8461 gctaacctca cggttaatcc gatctcagta ttaccatgag caagaactat tgccttttat
     8521 ataactctta agggttacaa gagtgaatgt aaatggtaat ataatgaaat ggtacctgcc
     8581 cgtaatgacc ataaagaatc tatcccggcg taaaagatat agagaataag atgcagtaat
     8641 gctgatgttt tgtta
//
LOCUS       DAZGCQ010000172         8636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000172 DAZGCQ010000000
VERSION     DAZGCQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.172"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHK07_004727"
     CDS             <1..234
                     /locus_tag="ACHK07_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104951.1"
                     /translation="TLPADANYKDKTLLLKAIYSGCTNLPFSLFINNEQLLEIRECCK
                     QAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            227..2287
                     /locus_tag="ACHK07_004728"
     CDS             227..2287
                     /locus_tag="ACHK07_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8104952.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILD
                     QLEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            2319..3611
                     /gene="sctN"
                     /locus_tag="ACHK07_004729"
     CDS             2319..3611
                     /gene="sctN"
                     /locus_tag="ACHK07_004729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO8104953.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            3601..4059
                     /locus_tag="ACHK07_004730"
     CDS             3601..4059
                     /locus_tag="ACHK07_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO8104954.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            4037..4267
                     /locus_tag="ACHK07_004731"
                     /pseudo
     CDS             4037..4267
                     /locus_tag="ACHK07_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            4565..4912
                     /locus_tag="ACHK07_004732"
     CDS             4565..4912
                     /locus_tag="ACHK07_004732"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO8104955.1"
                     /translation="MFTQHIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQE
                     NEGKFENDQKDES"
     gene            4872..5859
                     /locus_tag="ACHK07_004733"
                     /pseudo
     CDS             4872..5859
                     /locus_tag="ACHK07_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            5849..6514
                     /locus_tag="ACHK07_004734"
     CDS             5849..6514
                     /locus_tag="ACHK07_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8104956.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNGSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            6523..6783
                     /locus_tag="ACHK07_004735"
     CDS             6523..6783
                     /locus_tag="ACHK07_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8104957.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            6785..7552
                     /gene="sctT"
                     /locus_tag="ACHK07_004736"
     CDS             6785..7552
                     /gene="sctT"
                     /locus_tag="ACHK07_004736"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO8104958.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLGIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            7561..>8636
                     /locus_tag="ACHK07_004737"
     CDS             7561..>8636
                     /locus_tag="ACHK07_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO8104959.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDEQFSSEDKFIEG"
BASE COUNT         2739 a         1414 c         1836 g         2647 t
ORIGIN      
        1 acccttcccg cagatgctaa ttataaagac aaaacgttac tcctgaaggc gatttacagt
       61 ggatgtacaa atcttccatt ttcacttttt attaataatg aacaattatt agagattagg
      121 gagtgttgta aacaggcaat aaaagtaaca tttgcagcag agctctttga tacacaaaac
      181 tgtaacaaaa aacaaaataa aaaaccatgg aagaaggtga tgttcaatgt ttaacaaagt
      241 tttggtaggg ttaaggagtc atccagaatt aataattctt ggattaatgg tgatgattat
      301 cgccatgctc ataattccat tgcccaccta tttaatcgac tttttgattg ggcttaactt
      361 aacattggca atacttgtat ttttaggatc attctatgtt gataggatct taagcttttc
      421 ttcatttcca tcaatcctcc ttatcacaac gttatttcgt ttggcgctgg cgatcagtac
      481 cagtcgactt atacttcttg aggcggatgc tggagaaatt ataacgagtt ttggtgagtt
      541 cgttattgga gatagcctgg tagttggttt tgttattttc tccattgtta ctattgttca
      601 gtttatcgtg attactaaag gttcggaacg tgtggctgaa gttgctgccc gcttttcgct
      661 tgacggtatg ccaggtaaac aaatgagcat tgatgccgat ctacgcgctg ggatcattga
      721 tgcagattta gcaaaagaac gtcgtagcgt attagaacga gagagtcagc tgtatggttc
      781 ctttgatggt gcgatgaaat ttatcaaagg tgatgctatt gcaaacatta ttattatctt
      841 tgttaatatt atcggtgggc tatcagtagg agtaggccaa aatggaatgg atttctcaac
      901 agcgttgact gtctacacaa ttcttactgt cggtgatggt cttgtttctc aaattccagc
      961 tttattaatt gctattagcg caggatttat cgttacgcgc gtaaatggcg atagtgataa
     1021 tatggggcaa aatataatgt cccagttatt aagtaattct tttgtcatta ttgtgacatg
     1081 tgtcctcgcg ctaagtattg ggttacttcc aggttttccc cttcttgtgt ttttatgttt
     1141 ggcagtgatc ttaggaattt acttttactt taaattcaaa aagaaaggga ctgaagaaac
     1201 ggttgttgag ggagatatta ccgttgggtt ggaaccctat aatcaagatg atgacatctc
     1261 attaggtatc attaataaac ttgatcaggt tattaccgaa actgtaccct tggtcttgat
     1321 tatgaatagt gtacaggcta aaaaatacac tgaaatcaat cttgctgata ggattcgtag
     1381 tcagtttttt attgagtatg gtatccgcat tcccggtatt gtcattcgtg agggggaagg
     1441 tcttaatgat gaagatgtca tattaatgct aaatgaagtc agagcttcgc aatttaaaat
     1501 ttatcatgat cttgtcttgt tggttgaata ttcagatgaa gtggtttcga ctttgatcaa
     1561 gaaacctgtc attgtaaata gtaatggtga acaatattat tgggttacga aaagtgatgc
     1621 tcaaaaatta acaaaaattg gctgttatac tcgcacggcc atggatgaaa tgtataatca
     1681 tttatctgta tgtctggctc ataatattaa tgagtacttt ggtatacaag aaacaaaata
     1741 tattcttgat caattagaga tgaagtatcc tgatctatta aaagaaatat taagatatat
     1801 aaccgtgcag cgcatttcag aggtaattca gcgtctgatc caggagcgaa tttcagtgcg
     1861 caatatgcgg ttagttatgg aggccttagc attatggagc ccacgagaga aagatataat
     1921 cacactcgtc gagcatgtac gaggtgcatt aggtcgctac atttgccata agttttcata
     1981 ttctggagaa attaaagcaa ttgtgatttc gcctgaaata gaggacagaa tcagagatgg
     2041 tgtcaggcct accgctggcg gtacttttct taatctcgat gcatccgaag ctgagatgat
     2101 tctggataat tttaaactgg cactttctgg aatcaatatt cctattaaag acattatttt
     2161 actgggatct gtggatattc gtcgttttat caaaaaactt attgaatcta gttatcgaga
     2221 cctcgaagta ctttcgtacg gggaactaac ggaaaatgtt cccgttaata ttctgaaaac
     2281 tatttaggat aatgtcttgc gatatggagc atgctggtat gaaaaaatta aaattattaa
     2341 ataaatacag ctacttacat agcataaacg gctctctgat tgaagcagaa cttgacgacg
     2401 tttctgtggg tgaagtatgc gaaatttatg cgagctggca agcaaatgaa cgtatagcca
     2461 gggctcaggt cgttggcttt cgcaatggaa agacattatt aaaccttata ggcagctcag
     2521 tggggcttac acgtactgca gttttgaaac caacgggaga gcagttaacc atacagatca
     2581 gtgatgcctt tcttggttcg gttttgaatg cctctggtca aattatggaa aggtttgtcc
     2641 cggacactcc aggcgataga ggaaatttac gcctgattga tgaactgcca ccgtcgtatc
     2701 aggaacgtcg agttattaat acaccactag agacacgcat aagagtcatt gatggcgtac
     2761 tgacatgtgg cattggtcaa cgtgttggga ttttcgcatc ggctggctgt ggcaaaacgg
     2821 ttttaatgca tatgctcgta aacaatactg aagctgatgt gtttgttatt ggcttgattg
     2881 gcgaacgtgg tagggaagtt acggaatgcg cggaatcgct gaaacaatca gtaaatgcgg
     2941 caaaatgcgt attggtttat gccacctctg atttttcgtc agtagatcgg tgtaatgccg
     3001 ccctgatggc gacaaccgtg gcagagtatt ttagagatcg aggaaagaga gttgtacttt
     3061 ttattgactc aatgacgcgg tatgcacgag cactacgtga tatgaaactg gcagcgggtg
     3121 aaccacctgc cagacgtggt tatcctgctt ctgtttttga cagtttgcct cgcttactgg
     3181 agaggccggg accaacacta aaaggaagta tcaccgcgtt ttataccgtg ttactggaag
     3241 gggaagatga atcagaccca ctgggtgatg aaattcgctc aatccttgat ggccatatct
     3301 atctaagccg caaactcgcg ggtcaaggtc actatccggc aatagatgtt ctaaaaagtg
     3361 tgagtcgagt atttggtcag gtcactgatg aaaaacacag agataatgct gcacgggtaa
     3421 ggaagatcct cacaactttg gaagatctac aggtttttat tgacttaggg gaatatcgcg
     3481 ctgggcaaaa tgctgaaaat gattttgcta tgaatgcacg gcctaaactc acaaattggt
     3541 tgaaacagtc agttaatgaa aaaatgccaa tgagtgaaac tctaaaagag cttgaacgaa
     3601 ttgttaagta aagttaatcg acttataaga agaacagcgc agagcctggc tgcatgtgaa
     3661 gcatcattac aaaagttaaa tgcagaaaaa gaaaagttag ccgaaaagga acgcctgtat
     3721 gatatgcaac ttaaaaacct gcagtcactg ttagatatga aagagttact cggcgaagtt
     3781 gtatttcgcc aggatatatt ttactcgtta cgtaaagttg cagtcattca gcagcagatc
     3841 gctgagataa atcttgaaaa gcaaaaaata gcagagcgga gaaaaatact taacaaggaa
     3901 atagttcaac agcaagcaca aagaaaacac tggtggctaa aaggagaaaa atacgaacga
     3961 ctaaaaacgc gcatcaaaaa acaactttta gatcagatgc tttatcaaga cgagcttgaa
     4021 caggaggaaa aatataatgg acgaagtcaa gaaaattgaa cacggtaaaa ttttaaacac
     4081 agacacaccc gataattctg cttcggattt agacagtttc ttaaagaaaa acaaaaaaaa
     4141 taaagaaatg agtgagattg tcgctggaac cattctccag ttattacgtc agactcagga
     4201 tagccaattt tctacgttgg ggaaaaaaaa tgggcaaatg aagtctattg acaacaaatc
     4261 atctctccag gttgacagga atccctctct ttctttgcaa acaaatggaa cagagcaatt
     4321 ttctacaaag aaaattctca atatgacttc tcgaggtcag ggtattagtt ctgaactatc
     4381 tgataacacg ataaaaatta aggaaaaaat ccataatggt agccatacag agtacatcac
     4441 agatcaaaaa aacagtaata ataaagatcg tgaaaaaaaa taccgcgatg gagacaaaat
     4501 aaatggaccg caggctcatt cattagacag tactaacgaa agaaggtttg ctgataacag
     4561 aaccatgttt actcaacata tcgaaaagca aaggaatgtt catacgctca atcagaatga
     4621 tataaataat tccgttaata gtgtcaatgt gccagaaaat gaattgacat atcagtttca
     4681 gcgttgggga cagaatcata cagtccggat tctggaaagt agtgaaggga tacgactaaa
     4741 accatcagac gcgcttgtct ccgacagact ccatgaagcc caaaacaatg atgttacagc
     4801 acaacgttgg gtattgactg agcaagatga acgacaagga caacgacatc agcctcatga
     4861 ggagcaagaa aatgaaggca aattcgaaaa cgatcagaag gatgaaagtt aatgttcggt
     4921 ctgaggaaag taaatcgaaa cactcatacg tttgaaacca cttttcaaaa ttggaaagaa
     4981 aatggcgaag atgttgcatt attaatgccc gaatttagtg ctaaatggct accaatagca
     5041 gaagagagtg gcagctggtc tggatgggta ttgcttcgag aaatatttcc cttaatttct
     5101 tctgagctcg ctgggatggc tttaatgcca gaaacggaaa ggttaatagg ggaatggctc
     5161 agtttatcca gttccccgtt aaatctaaag catcctgaac taaaatataa tcgtttgtgc
     5221 gtaggtaaag tatttgatgg agtattgagc ccagctcaac cactaataag aatatggaca
     5281 ggggaattga atctttggtt agataaagtc acagtctgcc aatacggaaa cgctccagcg
     5341 ttagacaaaa aatcattata ttggcccatt cattttgtaa ttggatttag taaaacatgt
     5401 tacagaagtc ttgttgatat tgaagttggt gatgttttat taatctcaaa taatttggct
     5461 tatgcggtta tttataatac aaaaatttgt gatttaattt atccagagga gttaaaaatg
     5521 gctgatcatt ttgagtacga ggaagatttt gaaacagatg actttgatat caaaaaaagc
     5581 gaaattgaga ttaataatga aaatgacgat cagatgatta atagttatga agagttgcct
     5641 gtaaaaattg agtttgttct tggtaagaaa gtaatgaatc tatatgagat agacgagctc
     5701 tgtgcaaaaa gaataatatc tctgcttcct gaatctgaga aaaatataga gatacgtgta
     5761 aatggcgcgc taactggcta tggcgaactt gttgaagtgg atgataaatt gggcgtagaa
     5821 attcattctt ggttatcaag gaataataat gtcaaatagc atttcgctga tagcgatatt
     5881 atcattattt acactattgc cgtttataat agcatccggg acatgtttta ttaaattctc
     5941 gattgttttt gttattgtcc gaaacgcgct gggacttcag caagtgccat caaatatgac
     6001 acttaatggg gttgctttgc ttttgtcaat gttcgtaatg atgccggttg ggaaggaaat
     6061 atataataac agtcagaatg agaatctttc attcaataat gtagcgtcgg ttgttaattt
     6121 tgttgagaca ggcatgagtg gttataaatc atatttgata aaatattctg aaccggagtt
     6181 ggttagcttt tttgaaaaga ttcagaaggt gaatggttct gaagacaatg aagagataat
     6241 cgatgatgat aatatttcaa ttttctcgtt actccccgca tatgcattga gtgagataaa
     6301 aagtgcattt attataggtt tttatatcta tttacccttt gttgtagtgg atctggttat
     6361 atccagcgtt ttgttaacgt taggtatgat gatgatgagc ccggtaacca tatctacacc
     6421 gataaaatta attctgtttg tcgctatgga cggttggaca atgttatcaa aaggactcat
     6481 tttacaatat tttgacctat ccattaatcc ctgagaaaaa atatggacga tattgttttt
     6541 gcagggaata gggcgctgta tctgatttta gttatgtctg caggacctat cgcagttgcg
     6601 acatttgttg gtttattagt agggcttttt caaacggtaa cacagttgca agaacagacg
     6661 ttgccatttg gcgtgaaatt attatgcgta agtatttgct tttttttaat gagcggatgg
     6721 tatggcgaaa aattatattc ttttggtatt gaaatgctga atttagcatt cgcgagagga
     6781 tagcatgggt gaggcgatac tctatcagtt gcatagtctg ctggctgcta cagcgctcgg
     6841 tttttgccga ttggcaccca ctttctattt gttaccattt ttcgccagtg ggaatatacc
     6901 aacagtagtc cgtcatccaa taataatagt ggtatcatgt gcattagttc agcattacca
     6961 ctatgaatta ttgaacttaa atgaaatcga tattgctttg ttcgctgcca gagaaattat
     7021 catagggctg tttattgcat gcttattagc atccccattt tggattttcc ttggtatagg
     7081 tagctttatc gataaccaac gcggtgcaac attaagtagt actttggatc cggctacagg
     7141 tgtcgatacc tcggaattag caagattatt taaccttttc tctgctgcgg tttatcttac
     7201 taacggtgga atgaacttta ttctggaaac tctttggcaa agttataatt tatggccttc
     7261 aggtaatttt aattttccta aattagagcc tttattctca tatattaata acataatgac
     7321 tcatactatc gtttacgcaa gccccgtgat tgccgttatg ctgggcggtg aggctgtcct
     7381 gggattgctg gcacgttatg cttcacaact aaatgctttt gccatttcac tgactgtcaa
     7441 aagtgcgctg gcatttttaa tattaattat ttattttggc ccaatactgg ctgaacgagt
     7501 aatgccgtta tcctttttcc cagaacaatt gcaactatac attgagaaat agagtgtttc
     7561 atggccaata aaactgaaaa gccaacacaa aagaaactcc aggatgcttc taaaaaaggg
     7621 caaattctaa aaagtagaga cttaaccgtc tctgtaataa tgcttgtggg tactttatat
     7681 ctcggatatg tttttgatgt gcatcacatt atgtcgattc ttgaatacat ccttgatcat
     7741 aacgctaaac cagatatttg ggactatttt aaagctatgg ggattggttg gttgaaaacg
     7801 atcattcctt ttttgctggt ttgcatgttc acaacaatac ttgtctcctg gtttcaaagt
     7861 aaaatgcaat tagcaactga agctgttaaa tttaagtttg attcattgaa tccagtaaat
     7921 ggcttaaagc gtatatttgg cttaaaaacc gtaaaagaat ttgttaaagc aattctttat
     7981 attatttttt ttgcattggc gatcaaagta ttttggagta atcataaatc actgcttttt
     8041 aaaactcttg atggagatat catatcttta ttatcagatt ggggagagat gctattcctt
     8101 ctcatactgt attgtctcgg cagtatgata attgtcttaa tttttgattt tatcgctgaa
     8161 tattttttat ttatgaaaga tatgaaaatg gataaacaag aagttaaaag agaatacaag
     8221 gaacaagaag gaaatccaga aattaagtct aaacgcagag agcgccatca agaaattctt
     8281 tctgagcaat tgaaatctga tgtcagtaat agtcgtttga tgattgccaa ccctactcac
     8341 attgcaatag ggatatactt taagccacat ctttcaccta ttccattgat ttctgtaaga
     8401 gaaactaatg aggtagcctt agccgtaagg aaatatgcaa aggaaatcgg aataccaatt
     8461 atcacagata aaaaattagc acgaaaaatt tatgctaccc atcgtcgcta cgattatgtt
     8521 agcttcgaaa atatagatga aatattacgt cttctgctgt ggcttgaaga tgtggagaat
     8581 gccggacaac ctgttccaga tgaacagttc tcttcagaag ataaattcat tgaggg
//
LOCUS       DAZGCQ010000173         8623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000173 DAZGCQ010000000
VERSION     DAZGCQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.173"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..1107)
                     /locus_tag="ACHK07_004738"
     CDS             complement(106..1107)
                     /locus_tag="ACHK07_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8104960.1"
                     /translation="MSVRKLSTGKWLCECYPYGAAGKRIRKQFATKGEALSYERRLMN
                     SNLGGEFQDGSGPRLSELVARWFEMYGKTLSSGEERKAKLEAICSRLGDPFASQFDKN
                     MFAIYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYADEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQILP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDTAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1142..1399)
                     /locus_tag="ACHK07_004739"
     CDS             complement(1142..1399)
                     /locus_tag="ACHK07_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104961.1"
                     /translation="MVTNPQIPLTGLYVSKVNPSNRIVVTDVHIVLDDDDEPGDLPFY
                     LVTFVNEGDEDDMSAPSWELDPDEWEQLVDEKLFMRVEQSS"
     gene            complement(1597..2238)
                     /locus_tag="ACHK07_004740"
     CDS             complement(1597..2238)
                     /locus_tag="ACHK07_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104962.1"
                     /translation="MVVNSNGSDNRVAGRDYHEKNIQIERYDGRHTVNIAIPSNRTDE
                     RPLVKAQRRQLNLLIDNIVDVSKEESFVVWRKLHAEIGVDSIDNMTVNQYPTAVSFLN
                     AMLDRYKDHDACKSLVSLLLRNSENNEVRQKLLRYCHINFGTGRLNDLTRSQLQMALS
                     WLDQQSVEGEKKLTANNSAGSQLQMNFQQIIKKYPKETGMCFVIGVLLGIILF"
     gene            complement(2254..2700)
                     /locus_tag="ACHK07_004741"
     CDS             complement(2254..2700)
                     /locus_tag="ACHK07_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8104963.1"
                     /translation="MVCLVIMRSIDLFSQGVKVNFSSLCGARLKAERKRLSLKQADAA
                     ALCGVSRESWGKYERGTMVPGGDVLLSFALNGANVQYILTGEAGGVVLNRDEMEIIKH
                     FRNAPLAVKAAALAALTAGSSASNTVNVSGRGHRIAGRDYNETKGR"
     gene            complement(2737..2874)
                     /locus_tag="ACHK07_004742"
     CDS             complement(2737..2874)
                     /locus_tag="ACHK07_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104964.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSADNRSASRGPVAVLRGIRDLV
                     S"
     gene            2946..3149
                     /locus_tag="ACHK07_004743"
     CDS             2946..3149
                     /locus_tag="ACHK07_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8104965.1"
                     /translation="MQGITININVNAPYVSLKKYAEITGIPLNTCKKMLADGRIIIRP
                     KRARMEKPEVNLIAMLKDAIANS"
     gene            3170..3520
                     /locus_tag="ACHK07_004744"
     CDS             3170..3520
                     /locus_tag="ACHK07_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO8104966.1"
                     /translation="MKKNAIPPRPKHRTGTERHANRFSTSASRSNSHYSLQETHATPD
                     GHLVKQTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTAD
                     RLISGRSFQHPWGN"
     gene            3524..3778
                     /locus_tag="ACHK07_004745"
     CDS             3524..3778
                     /locus_tag="ACHK07_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104967.1"
                     /translation="MATTTKDMAEIASAYTIIIHRLIANNARNALNTAKPLWETKKDI
                     ISALQGLQKCADLAGDYAVYMAIEDTIEKIESGKSLSDFV"
     gene            3799..4041
                     /locus_tag="ACHK07_004746"
     CDS             3799..4041
                     /locus_tag="ACHK07_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO8104968.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALGNAA"
     gene            4038..4163
                     /locus_tag="ACHK07_004747"
     CDS             4038..4163
                     /locus_tag="ACHK07_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104969.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            4243..4731
                     /locus_tag="ACHK07_004748"
     CDS             4243..4731
                     /locus_tag="ACHK07_004748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104970.1"
                     /translation="MSINTPTEINKLFTDPAHIESTQKMLTEITNALDERISGIDKDN
                     HSSTYMTLKIQSMAMVIANQTISELYMKNLRLERELAELRAQMKTDESPTGEINARPE
                     HTNAHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAILTNPDELKG
                     GA"
     gene            4731..5312
                     /locus_tag="ACHK07_004749"
     CDS             4731..5312
                     /locus_tag="ACHK07_004749"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8104971.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNEHCMRPLIDMAESTGWRMPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            5350..6324
                     /locus_tag="ACHK07_004750"
     CDS             5350..6324
                     /locus_tag="ACHK07_004750"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO8104972.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRAARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHAFLSTRISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPCNGRDNFTAYG
                     KPFNEGEHIRLRATLDHLSQETDGMTCITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDACGRHGGGYCPDCGPVTGDATYCVMFTPAST"
     gene            6360..6596
                     /locus_tag="ACHK07_004751"
     CDS             6360..6596
                     /locus_tag="ACHK07_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104973.1"
                     /translation="MNSERNKALLRGLRHIARAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQEREIAALKKLLHGKSAGQGVKQ"
     gene            6593..6799
                     /locus_tag="ACHK07_004752"
     CDS             6593..6799
                     /locus_tag="ACHK07_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104974.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCRRAL"
     gene            7136..7363
                     /locus_tag="ACHK07_004753"
     CDS             7136..7363
                     /locus_tag="ACHK07_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO8104975.1"
                     /translation="MANRRQQRARAERLHTRTEINRRLWRASRVAQIMYVNMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQNHH"
     gene            7432..7842
                     /locus_tag="ACHK07_004754"
     CDS             7432..7842
                     /locus_tag="ACHK07_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104976.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     CITTYGDFSFTVDGRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQVATAGECDA"
     gene            7839..>8623
                     /locus_tag="ACHK07_004755"
     CDS             7839..>8623
                     /locus_tag="ACHK07_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8104977.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLARHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTDP"
BASE COUNT         2450 a         2298 c         1933 g         1942 t
ORIGIN      
        1 actatcaacc accatttata aatcataacg ttatgatttt attaagtata aagataaagc
       61 aatatggcgc tatttgatgc ttttgttact tttcatcgcc actttttacc ctctattgtc
      121 aaagggattc agttcaacag cagcctctaa atgacccggc gcaaagtgtg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaaggatat ttcccccccg
      241 catcataaaa tggctggcaa acgtatgacg tagcacatga gttaattgcc catcaggaag
      301 ttcgatcttt gctctcttaa tagcggtgtc aaaagcctca taacatggtg aaaaaagcgc
      361 cccgcgtttt ttaggaagca tggcctgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttcctttg gttttaacaa atgtgattcg tccgggcagt atttgagatt gctttaatcc
      481 ctctgcttca ctccacctgg caccagtcgc aagcccaata cggacgacaa cacccaaatc
      541 tttattccgt gactcatcac atgcaatcag aaggcgttca atctcatctg catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacct gtcaatggat tttcaccaga
      661 ccactcccca aggcgcttca gttcagcaaa aacagcatgt aaatatgact gttcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc ctttggattc cattcacctg ataatcgccg
      781 ttccctgtag atggcaaaca tatttttatc aaattgagaa gcaaacggat cacccaacct
      841 agaacaaatc gcctcaagtt tagcctttcg ctcttcaccg gaagacaacg ttttaccgta
      901 catttcaaac cagcgagcaa ccaactcgga aagacggggg ccagaaccat cctgaaactc
      961 gccccctaaa ttactgttca ttaaacggcg ctcataagag agcgcctcac cttttgtagc
     1021 aaactgctta cgaatgcgtt ttcctgctgc tccgtaggga tagcattcac agagccattt
     1081 accagtagat agtttacgaa cagacatatt aaaaagtgca caccaaggaa aattttcgtt
     1141 attaagatga ttgctcaacc cgcataaaga gcttttcatc aactaactgc tcccattcat
     1201 caggatcaag ctcccaagaa ggagccgaca tatcatcctc atccccctca tttacaaaag
     1261 taaccaaata gaaaggaaga tctccgggct catcgtcatc gtccaggaca atatgaacat
     1321 cagtaacgac tatacgatta gatggattaa ctttagatac atataaacca gttaatggaa
     1381 tttgcggatt agttaccata attcatttac ctctttggac ttcctcatga ataaagactt
     1441 acttttcttt aagaccatga caagcaactc acaccatttc ttacatcact agttgcccct
     1501 ctcacctttg tcagctcgct taatttatct tcttcatttc ttttaaagtc ggtaaagcaa
     1561 ccttttgcca taatatcttt ctccaacaac tatctatcag aaaagaataa tgcctaataa
     1621 aacaccaata acgaaacaca ttcctgtttc ttttgggtat tttttaatta tttgctgaaa
     1681 attcatctgt aattgactac cggcagaatt gttggcagtt aatttctttt ctccttccac
     1741 agattgttgg tctaaccagg aaagagccat ctgaagctga gaacgagtaa gatcgtttaa
     1801 acgccctgta ccgaaattga tatggcaata ccgcagaagt ttttgtctaa cttcattatt
     1861 ttcactgttg cgaagaagga gacttacaag agatttgcag gcgtcatgat ccttatatcg
     1921 gtcaagcatc gcattgagaa aactaaccgc ggttggatac tgattcaccg tcatgttatc
     1981 gatactatca acaccgattt cagcatgaag tttacgccaa acaacaaacg attcttcctt
     2041 gctaacatca acaatgttat caatcaacaa gttgagttga cggcgctggg ctttaactaa
     2101 tggacgctca tcagtcctgt ttgaagggat tgcaatatta actgtatgac gaccatcata
     2161 ccgctctatc tgaatattct tttcgtggta atcacgccca gcaacgcgat tatctgaacc
     2221 gtttgagttg acgaccatct cacctcccta ctatcaccta cccttagttt cgttatagtc
     2281 acgacctgca atacgatggc ctctacctga tacgtttacc gtatttgatg cagaactccc
     2341 cgcagtcagg gcagcaagag ctgcagcctt tacggcaagc ggagcatttc taaaatgctt
     2401 gataatttcc atctcatcac ggtttaggac aaccccgcca gcctcaccag ttagtatgta
     2461 ttggacgtta gccccattaa gcgcaaatga gagcaaaaca tctcccccag gtaccatagt
     2521 tccccgttcg tactttcccc aagattctcg cgatacgccg caaagagcag cagcatcagc
     2581 ctgtttgaga gataaacgct ttctctcagc cttcagcctc gcaccacaaa gagatgaaaa
     2641 attcactttt acaccttgac taaagagatc tattgatctc ataataacta aacaaaccat
     2701 tagatgatca caatatacca ctatgaaaca agttcttcac gataccagat cacggattcc
     2761 gcgtaacaca gctacaggac cgcgactggc acttcgattg tcagcagaag agcgagccgt
     2821 cattgatgaa atggcagcta aagaacagcg ctcatcctct aacatggcac gcatgatctt
     2881 ccttcgcggc cttgagatta tccagaaaga gcaaaaccaa tcttcatgct caggaggtta
     2941 acgggatgca aggtataaca ataaacatca acgttaatgc cccttacgta tctctgaaga
     3001 aatacgccga gataacaggc attcctctta acacatgcaa aaagatgttg gctgacggtc
     3061 gaattattat ccgacccaag cgcgcaagaa tggaaaagcc agaagtaaac cttatagcga
     3121 tgttaaaaga cgcaatagcc aacagctaaa caataaggcg agcagaacta tgaagaaaaa
     3181 tgctattcct cccagaccta aacaccgcac tggcacagaa cgccacgcta accgtttctc
     3241 aaccagcgca tcacgcagca actcgcacta cagcctgcag gaaacgcacg ccacacctga
     3301 cgggcatctg gttaaacaaa ccggtaaaca cacctggctg attaatgatg ccggaattgt
     3361 catacacaga ggcgcacgca acccgattac cggaaatacc tgttactccc tgactcgcgg
     3421 agatgagcag ttcgggcagg atttcaccct gcacgaagca ttaagaacgg cagaccggtt
     3481 aatcagcggt cgcagttttc agcatccatg ggggaattaa tacatggcaa caacgacaaa
     3541 agacatggca gagatagcat cagcctacac aattatcata caccggctca tagctaataa
     3601 tgcccgtaat gcactaaata cagcaaaacc tttatgggaa acaaaaaaag atatcatcag
     3661 cgcattacag gggctacaga aatgcgccga cctcgcaggc gattacgccg tatacatggc
     3721 tattgaagac acgatagaga agattgaatc aggtaaatca ttaagcgatt ttgtttaatt
     3781 gttttccggg gaaatacaat ggacaaagaa tataaggttc tcatcaataa agcccttgag
     3841 cgctttaact ttcgcctgag tatgtcagga gttgccgcag aacatgcagc aagagactca
     3901 ctgaccagag caattcgcag cctgtatgac gcagcttttt acgctgacga tctggatgct
     3961 ctcaatgaac tgtctgagct tgttcgtgat gcagaatgcg gagtacatat tgagccatac
     4021 gcacttggga atgcagcatg agcgtatttc tttcatgggt gattctgatt atttccgtgc
     4081 ttatggcgat tggcattatg cgaattatcg aagcgctgaa aaatatcgaa tctttattta
     4141 ctggcgaaga acgccgtaaa taaataacca accggacaga aaacagtaaa accatccgca
     4201 tctgcggagg gcttcactca atccagaaac ggagaaacag ccatgagcat caatacacct
     4261 acagaaataa acaaactatt taccgacccg gcgcatattg aatcgacgca aaaaatgttg
     4321 actgaaataa ccaatgcact ggatgaaaga atatcaggaa tagataaaga caatcattct
     4381 tctacttaca tgactctgaa aatacaaagc atggcaatgg tgatagcaaa ccagaccatc
     4441 agtgaactct atatgaaaaa cctgcgcctc gagcgcgaac tggcagaact ccgggcacaa
     4501 atgaaaacag acgaatcccc caccggagaa ataaacgcca gaccggaaca tacgaacgcg
     4561 catatcacca tgatcacaag aaaagccgag cgtactcata tttgcgaatt actgcgcgag
     4621 ctgattgaga cagcgaaaga gacacacacc tgcccacagg aaacctgcgc agccattgag
     4681 cgactgattg caatactcac caacccggac gaactgaagg ggggcgcata atgaaccatc
     4741 tcatgattga caccgaaaca ctgggtaacg gaccggacgc agccatattc gcgatcgggg
     4801 cagtgttttt cgacccgttc accggaaagc tgggaaaaca gtttgagcga ttcattgacc
     4861 cggtggattc agagcgcaac ggtggaaccg tcaatgcagc aaccgctgta tggtgggccg
     4921 gacaatccgt ggacgccaga gcctgcctgc gcaatgcaga tggaacagag ctggcagcag
     4981 tgaccgaatt cctggcgttc atttcacgca atatgcatga cgaaagcccg ggcaagacac
     5041 tgaccgtatg gtgcaagggc gcttcgtttg acttcccgat cctgaaatcc gctatcacgc
     5101 gaacaaccgg agataaaagc atcccctggt gttactggaa cgagcactgt atgcgtccgc
     5161 ttatcgacat ggctgaatca acaggctgga gaatgccagg cagacaagga aaagagacca
     5221 ttgctcatac cgcactgaac gacgccattt accaggcgaa ggttgtttca gaaatctggc
     5281 agcgcttcac tgcgccattc ctgagcgtat gaagcggaca cacgaaacct gtaacaaaga
     5341 ggccgcgtta tgagcaaaga actgttatca ggcacaccac tgaaatgggt gggtggaaaa
     5401 agccgcatca tgaacacact gaaggcgcac ctgccagaag ccgactgtct ggttgagccg
     5461 ttcgtgggcg gcgcatcggt attcatgaac accagttaca gccgctacat ccttggagat
     5521 tccaatggtg cgctgattaa tttctggcgg gcagcacggg acaacaccga cagtctgatc
     5581 gacgaggcaa aagacctgtt ccgggaacac aacaacagga ctgactatct cgccatccga
     5641 aacaccttta atgcagagaa tcatgcattc ctgagcacga gaataagcga ccgtcgggaa
     5701 ctgcgactgg ccgccatgtt tttgtatctc aaccggcact gctttaatgg cctgtaccgg
     5761 gtgaacggaa aaggtgactt taacgttcct ttcggtggct accggactcc ctacttccct
     5821 gaacgggaaa tccgcgcatt tgcagacaag gcaaacagca cccgaacact gcttgttcat
     5881 ggcgattttc gaaaaacgct gagctgtgcc agccacctgt tcagcatggg taacacgctt
     5941 tgcgtgtact gcgatccccc ttacctgcct tgcaatggcc gggacaattt caccgcctac
     6001 ggaaaaccat tcaatgaagg cgaacacatt cgcctgagag caacgcttga ccatctttca
     6061 caggaaacgg acggcatgac ctgcatcacc atatccaatt ccgacacacc ggaaaccaga
     6121 cgtatttatc agggttaccg gatgaactgc atacaggcac cacgaagcgt tggcgcaaga
     6181 acccgcgaac cagcaccgga ggttatcgcc accctgaaac agtgcgacgc ctgcggtcgc
     6241 catggcggtg gctactgccc ggactgcgga ccggtaacgg gagatgcaac ttactgcgtg
     6301 atgtttaccc ctgccagtac gtgaataaac agatgaatca gtaacacagg agtcaggaaa
     6361 tgaatagcga aagaaacaaa gcacttttgc gggggttacg tcacatcgcc cgtgctcacg
     6421 aggaaacagc caaagttgca gctgccgttg gaaaaccaaa ggtcgttata tcttcctcga
     6481 tctcactggg gatcaccatg gaagcaatcc gggtgattca ggatcaggaa agagaaatcg
     6541 ccgcccttaa aaagctactc cacggtaaat ctgccgggca aggagtcaag caatgatcaa
     6601 ggagtcaacc gttatcagat tcacaaccca cggccatcag tacgaagtcg acgaaagcct
     6661 gattgatcag ggcatgacac gccgggattc acgcaatccc gatatatatc acatccgcct
     6721 tatcaacggt tcgcacttcc gcgccacgaa cgtggaagaa gtgcgcgtgc ttaccacaag
     6781 ctgccgtcgt gctctgtgaa ctgttaccgg gctgagtgat catcatgcac acacaaaaaa
     6841 accgcttgcc agcgttcgtc tggtcgggtt acagttttcc cgcacctcat aaaacgggtg
     6901 ccgggtttcg cagcctgccg actacaagag cgcacaaccg cgccagcggt ttttttgtgc
     6961 gtactgtatc gccacgtctt tttcgcgtca gaattatggc ggggcgtacg gggccgactt
     7021 cggtcgggcc gggatctctt gtagccggta ctgcgaacct cgtacgtctc gccacccaca
     7081 gtttcgcagc tctggatggt gagttttcag aacttactac aagaggccaa acaccatggc
     7141 aaaccgcagg caacagcgcg cccgcgctga gcgtcttcac acccggactg aaatcaaccg
     7201 cagactctgg cgcgcatcac gcgtcgcgca aatcatgtac gtcaacatgc tgcatgaaca
     7261 cgcacacgcg ctgtcagacc gttactgtgc cgccgtcttc agctacctgg cggatgacct
     7321 ggcagaactg caggacctta tcaaccagca aaaccaccac taaacaacat cctgaagcca
     7381 tcccgcaccc gtgcgcggga ggcttccgca cgtctgttac cggaggttct catgaagagc
     7441 gttaagggac tttcagatat tcaggatttt tttaactgga tgtgggcagt ggatccccgg
     7501 ctggcttccc gcctcaacga ctggcatgac tattaccgcc gcgcctgtaa acaggccgcc
     7561 agatgcatca ccacatacgg ggatttctcc ttcaccgttg acggccgtta ccgcgtggac
     7621 gtgaaaggtg atggttttgg tttatttcac atcaacggaa ccgacagcac cgccatcagc
     7681 tggtcgtcgt cagagatgac gcttgttctg gaaatgacaa cgcacagcgt ggccgtgaaa
     7741 ggcatataca ccgcagaaga ctttatcggt gaataccagc gccttctcgt gctgtaccgc
     7801 gcggcgtttc aggtggcaac tgccggggag tgcgacgcat gagcgaccat aatgcacaac
     7861 tggcgtaccc gtggaacgcc ccgctgccgg ttatcgatcc tgaaacgttc ggcagggcac
     7921 agccaaccgc attacgccag gccattcaga gctacatcaa cgaggacatt cgcactgacg
     7981 ccagactgga tgaagaaacc gtcgccttcc tgacaaccac agacgaaggg aagcgaatta
     8041 attcgcatct tcatcacgac gaagagcgtc ggatcagact ggaaaaactg gcgcggcaca
     8101 aacgcgaaaa tccgcccacg ctggtatctg aagccatggc agagctgcgc gccctgccgc
     8161 cgtttcttgc cggtccgctc atccgggacc tggcacgcct gagccgcaaa caggagagcg
     8221 cacgccggga agggcttaag aacaacagcg catacgcggc ggataacttc gttcgtcacg
     8281 ggctgcgtgc ccgcctgaaa cgtatcgccc gtgtgaatga acgctttgcc accccggcct
     8341 ttaaggccac cgccgcgcgc gaacggctgg atgaactgct tatgctgccg cagctgagcc
     8401 gtgacgaaat ccagcgcctt gccaccctga ccgccaccgc gttttccgcc gcactggagc
     8461 gcgcatgtga tgaggtcatc gaaaagaccg gcaggggtga cgatgacctg ttcacctggt
     8521 tactgaccta ccagccactg gcacacatgg cgttaaaact gggtatcacg ccaccctgct
     8581 ggccgtcgct ggaaatcagg cccgaccgcc gcaccgaccc cga
//
LOCUS       DAZGCQ010000174         8610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000174 DAZGCQ010000000
VERSION     DAZGCQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.174"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(357..623)
                     /locus_tag="ACHK07_004756"
     CDS             complement(357..623)
                     /locus_tag="ACHK07_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO8104978.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(620..1387)
                     /gene="yjiL"
                     /locus_tag="ACHK07_004757"
     CDS             complement(620..1387)
                     /gene="yjiL"
                     /locus_tag="ACHK07_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO8104979.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1397..2548)
                     /gene="yjiM"
                     /locus_tag="ACHK07_004758"
     CDS             complement(1397..2548)
                     /gene="yjiM"
                     /locus_tag="ACHK07_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO8104980.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQREEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2664..3944)
                     /gene="yjiN"
                     /locus_tag="ACHK07_004759"
     CDS             complement(2664..3944)
                     /gene="yjiN"
                     /locus_tag="ACHK07_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO8104981.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3985..5216)
                     /gene="mdtM"
                     /locus_tag="ACHK07_004760"
                     /pseudo
     CDS             complement(3985..5216)
                     /gene="mdtM"
                     /locus_tag="ACHK07_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            5683..6627
                     /locus_tag="ACHK07_004761"
     CDS             5683..6627
                     /locus_tag="ACHK07_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8104982.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            complement(6869..8281)
                     /gene="yjiR"
                     /locus_tag="ACHK07_004762"
     CDS             complement(6869..8281)
                     /gene="yjiR"
                     /locus_tag="ACHK07_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8104983.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8458..>8610
                     /locus_tag="ACHK07_004763"
     CDS             8458..>8610
                     /locus_tag="ACHK07_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO8104984.1"
                     /translation="MEFHKNKAKTPFIRLVLLWHAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLRRE"
BASE COUNT         2245 a         2268 c         2163 g         1934 t
ORIGIN      
        1 ccgagtgttg ggaaaatatc ctggtatatg tttagggagc ccatattttt atccttgaat
       61 tgtgaaatgt tcttctaaat ctaaaataat cagggggatg ggtaaagata agcttcgcac
      121 agaaacttat cttttatcat tatctcaccc ggtaatcatt ttatttctct atgcataatt
      181 atgggtttaa gtataataaa aagtatttta tattttagac gtaaaatagt aaggtgctat
      241 caaaaatcgc tcattttgtg gatgggaaat atttcacggc aggcgagata tttactacaa
      301 cttccttcat gcaggattat gcgcagccaa ttatccggtt ggtatattta aagatcctat
      361 tgcctggttg acgcttctgc atcgtaatgc accacgcact gccagtcgct gccctgctgg
      421 caatatatcg cttcggtttg cccagggatc acccattgta tttcctcgcc ggggggacag
      481 gtcagccaaa tgcataaccc gcagccgccg cgtaaatcac ggggaatatc gctgacgcga
      541 aaagtcatgc ccgctgcctg caacgccttg cgggtttgta tgacgccgac cgtggagtga
      601 aataaaaata aaaactcttt catcggcgtg tcctcactcg ttgaccaatt accgccgcgc
      661 caattgcgcc agcaaattgc gcatcaggat gggtatttac cggcattcgc aggtgagatt
      721 ccagcatccg ggcaaacgtc tggcaatgac taacgccacc agtaaacaga atcggcgctt
      781 cacaggagag acgagcaatg aaattggcac tccgccgcgc catcgcgtta atcactccgg
      841 cgagaatcgc ttctggcgcg acgcccgctg agcgcaggct gatcacttct gattcagcaa
      901 acactgtgca catactcgtg atggcgtgcg gcgtgacatt ttcggtaatg cagtcgagtt
      961 gctcgacgct ggtgccaagc gtgcgcgaga tcacctccag gaaacgcccg gtgcccgccg
     1021 cgcatttgtc attcatcagg aaatcgcaca ggttaccgtc atcatcaagc tgaatcactt
     1081 tgctgtcctg accaccgatg tcgattaccg cgcgcgttgc tggcgcaaga aaccgtgcgc
     1141 ccagcccgtg acaggagatt tccgtcactt gtttatcggc aaaatctacc agttgccgcc
     1201 cgtagccggt gagcgtcaga aacggcgttg tctctaaccc ttcgcgcaga gtttcccagg
     1261 cttcagtaat tgctgttgcc gggcgaaagg gggttggaac gaggaaacgg cgcgtaatca
     1321 cgccgtctgc cagtaagatc cctttggtgg cggtagaacc agaatcaatg ccaatcgaat
     1381 atgtcactgc cactccttac agcatctcaa taaaggccgc gacacgggta ctgagctgcc
     1441 cgacatctga ggtggagtag tctgtttcaa tagcgatata aggaatgttg tgctgctgac
     1501 gcacatgacg tttaattgcc agcgattcca ccgcgtaggt atggcacgcc tgcaaaatca
     1561 catcaactac gccatcgacc tgatattctt ccaccatctg gctgagcatt ttcaggcgct
     1621 gatcgttcgg cgaaacacag gagcagccaa tcgccagata tttatccgcc agcgcgtcgt
     1681 agacatcgcc cgtttctgcc acgcattgct cggtcgcttt cgccccggtg cagttttcat
     1741 aaccgacaac ccagccgcca ttctcttcaa tcgcgcgcac cactttctct gctgcgccgc
     1801 caatcgggca gccggtgatt aaaatgcgcg gacgcgggtc cagtcgctgg ccttcttccc
     1861 gctgctgacg aatgcgggcg gtcatcgcgt ccagttcatt gatcaacgcc tctttatcga
     1921 accggaaggt tgcgccgtaa accactttca gaatgtcgct gccgctaagc gccggaggat
     1981 ttaactgccc aagatgataa aaattggcca gtgcgcgacg ttcgcggttt ttcagcgcaa
     2041 tagcatcgcg cagagcatct tcgctaatct cgtgcccaaa acgttcttct accgtttttt
     2101 gcaagcgcag catctcggct ttccacaatg cacgcgaggc atcgtcctta acgctgttcg
     2161 gcagctgcat cacatgaaca ggcttaaact ccgccatgta ttcatacatt ttctttttgc
     2221 cgtcgcaggt ggtttcaccg accaccagat ccgaaaagta gaagtagggg catttatcgg
     2281 ttttgccgaa gccgtagctg cttttaatca gcgggcagag gttgcgcggc agatctttct
     2341 ccgcttcttc aatggtttca tcagaggtgg aacagagcga aaccacaacc gcaccggctg
     2401 ccatcgggat ctcttgcggc ataaaggtgc agtaagtgcc aaccagcgga atgccgcgct
     2461 ccttgagatc catgacggtg agaaagcctt tctggcgagc ttcagagaac tgatcgaaaa
     2521 tggcgggtag atcggtgaca agtgacatga ttttccttcc ccgtaccacg ggagataatg
     2581 aaaagaggcc acattattac cattctttta gtatggttta tttgatctct ctcgcggtta
     2641 gccacttagt ttttcatgga tttctaaaaa ttgccgaggg ggaacaaggc cgggagctgc
     2701 gacagcaaat ataaaattag cccaatacaa ccgccaacca gcgtaccgtt gacacgaata
     2761 aactgcagat ctttgccgat atttaactct atttgccgcg acatatcccg ggcatcccag
     2821 ctttttaccg tatcgctgat gtggcgcgtc aggaatgcgg aaaactcagg cgcgacgcgg
     2881 tgcgcggctt gttccagatg accatttaac gacgcccgca aggcatcatc ggcaattaac
     2941 gtttcgccaa accattgacc cgcccgcgcg atacgttctt tcacacgaga atcttcactg
     3001 ttgatatccg ctttcagcca ttcccgtaaa tccccccaca attcactgag atagcggtta
     3061 aaagcttcat cttctttcag ataacttttt acggcatcgg ctcgcgctgc catttccgga
     3121 tcgtttttca gcttgtcgat cagggcaaaa gtggcgcgat caaacgcatg gcggatctga
     3181 tgcgcacgat cgcggctgat atcatcaagc aaagaattca ccgcgtcaga aaccaactcc
     3241 gcgctatgtt cgcccagcca ttcggtgggg agaattttgg ctttcagtgg atgctcactc
     3301 tccagccagc gaacaatttg ctgggcgata aacttgcgcg atttatcgcg ctggagaaga
     3361 gcaatcaact gtgcgatcag cgtatccagc agcacctgat gacgatcgtt tttggtcata
     3421 ctctccagca tcaacgcact ggtgccggaa agatcgacct tatcaatcgc ccgatggacc
     3481 gcgcgcttaa gcaggcgctg aatacgcgca tcatcagtta attcgagaaa accactcatg
     3541 atctgcaaca gatgctgacc aacgcggcgg gcgttttctg gctggctaaa ccagttgcca
     3601 atcaacaacg ccggttcgtg gcgtcgaatc aatgccacca gggattgagt atcaagaaat
     3661 ttttcctgca cgaactggcc gagattttcg ccaatccggt ctttattacg cgggataatc
     3721 gccgtatgac gagaaataat cggtatcggc acgcggcgaa acagcgccac caccgcaaac
     3781 cagtccgcca gcgcgccgac catcgccgct tcagcaatcg ccttcacgcc gctcacccaa
     3841 aaattgggcg gcaaaaacag cgtaacgaca aaggtagcag cggcgataag cagtaaagag
     3901 agcgccaaca ttttggcgcg tctgagttca atgagtttat tcataacgtt aagaatagag
     3961 cctgacggca ggatcgcgca aaaatcactg ctcctccgcc agctcggcag cctgatgttg
     4021 gcgcacgcga ttgagcaatc ctgccagggt gaaaacgacg ataacgcccg ccacgacggc
     4081 taacagatga aacggcaagc gaccgccgtt aaaccacagc cagcggccga tttcgaccga
     4141 gacagacatc accatcagga tcaccatatt cagcgatgcg gagacggtcc ctttcggtaa
     4201 gttattggaa aacagcgtaa agcggataag gtcgggaaaa tcaaaccaat cccgaaagca
     4261 tacagactgg tgcccagcat cgaccacagc cagacgtgcg gtgacagcag attgccgata
     4321 atcaacagcg cgaggccaac cagttgaatg ggtacggcac gccagataaa gcgcggttcg
     4381 gtcggatctt taacgaaacg cgccacgatg gcattcgcga caatcaccgc gccgaacacc
     4441 ggaacctgtg tccaggcgaa ctgcgaggtc gttaagccgc ctgcgtcaat caggatcacc
     4501 ggcgagacag ccacccaact catcatgggg atatagctta aagagatggt tgctgcgcca
     4561 aagaggaaca gccgattgca aaagacatta cgaaaatcgc gcaagacgct tttggcgcta
     4621 aatggaaccg cgccgcgctt caccgtctct ggcatcgcca gcagtaagcc aacaaatgag
     4681 ataaaaccca taaccgcaat gatggcaaaa aggactttcc agtgcacaaa gtgcatcaga
     4741 gctgttccgg aaagcgggcc aataatcggc gcaatcagta cgatggaggt gataatcgcc
     4801 atcaacttga tcccttttgt ctgtccgaac gcctcctgca ccgtgacata accaacggta
     4861 gcaataaaac agatactggt gccctgaatt gcacgcgcga taagaaactg tgtcatagac
     4921 gttgtgaaca ttgtcgcagc gcaggcaagg gtaaaaatga gcgccccggt aatcagcact
     4981 ggcctgcggc caatcctgtc ggaaagtggc cccagcagcc actgtaacgc cataccgcca
     5041 gcgagataga gactgacggc agcaggggcc agactgacat cggcattaaa atcacggacc
     5101 acattaatga tccccggctg gatcagatcc gtcgaaagat aggcggcaaa gtcatacaaa
     5161 atcaacgcca tcgggaaaaa cagcgtagcg gcatggcggg caaaaaaacg tggcataaca
     5221 aacaactcct tgtccggaga accccaatgg ggaaaacgaa attcaatacc gtcggtaatg
     5281 acgaaaggca ggcgagaagg acaaatgtac tgaatttcat gtaatcaatc acactgcctg
     5341 tgaacacaca gacagagaaa taccttctga cttcgggtca gaatgtataa cgattacaaa
     5401 tagtcatcag cacattacac ctcttgaatt gatgggttgc atagaaaata tacaccttaa
     5461 gtgtaattaa aatttgcggt taatcaaaag agcgcggtgg aaaggggaat atctgccggg
     5521 tacaggacat gtaaaacgcg agcctgtttc cggaaaaatg tatcgtttgc agacgaacaa
     5581 aaatagcctg gaaagcacct cggggaacga gaaattgccg ggtgagaatg gttttgttag
     5641 tcgctaaagt cgggccatct tatctacagg tgacggatcg ccatgacaaa cttcacgacc
     5701 agcacgccac atgacgcatt atttaagtcc tttctcatgc accctgacac cgcgcgggat
     5761 tttatggaga tccacttacc caaagattta cgtgaactgt gcgatctcga cagcttaaaa
     5821 ctggaatccg ccagctttgt cgatgaaaaa ttgcgggcgc tacactccga tattctgtgg
     5881 tcggtaaaga cccgtgaagg tgatggttat atttacgtag tgattgaaca tcagagccgc
     5941 gaggatatcc atatggcctt tcgcctgatg cgatattcca tggcggtgat gcagcgccat
     6001 atcgagcatg ataaacgccg gcagctaccg ctggtcatcc cgatgctgtt ttatcacggt
     6061 agccgtagtc cttacccctg gtccctgtgc tggctggacg aatttgccga cccgactact
     6121 gcacggaaac tttatactgc agcgttcccg ctggtggatg tcactgtcgt gccagacgac
     6181 gagattgtgc agcaccgcag agtcgccctg ttggagttga tccaaaagca tattcgccag
     6241 cgcgatctga tggggcttat cgatcaactg gtaatattac tggttacaga gtgtgctaat
     6301 gacagccaga taactgcgct gttaaattac attttactga ctggcgatga agcgcgtttt
     6361 aatgagttta tcagtgaact tacccgtcga atgccacaac aaagggagcg aataatgacg
     6421 attgcagagc gaattcataa tgatggatgg ctgttaggga tggaaaaggg gaaagaagaa
     6481 ggggaacaac gcctccttag attgcttttg cagaacggtg cggatcctga atggatacaa
     6541 cgatatactg gactttcggc agagcaaatg caggcattag agcaaccctt gcctgaaagc
     6601 aagcgcgatc catggatcga gtactaatca gagactgatg acaaacgcaa agctgcctga
     6661 tgtgctacgc ttatcagacc tacattacct ctgcaatcta ttgaatttgc gcgatttgta
     6721 agccggataa ggcgttcacg ccgcatccgg tatgaacaaa gcacactttg cctaagccat
     6781 gctggaacgt ggcgatgccc ctgcaaagat aaaacgagtc acaggcgatt cgtgagagga
     6841 aatcaggggc ctcgccacta attcgaattc attccattgc ctgatacacg gcctcgccaa
     6901 tttgcttcag tgcttcgcga taggtttcgc tgagcggcaa agcgcagttg atacgtagac
     6961 aattacggta tttgccggaa gccgagaaaa tcgagcctgc cgccacctgg attttcatgc
     7021 ggtatagctg ccgcgcgacg cagaccatat cgacctgttc aggcaattcg atccacagta
     7081 aaaatccgcc tttcgggcgc gtaatacaga tttcgcaggg aaaatattcc cgtatccagc
     7141 aggtataaag cgccaaattg cgctgataga tctgccgcat ccgccggata tggcgatgat
     7201 agtgaccttc cagcacaaac gtcgccgccg ccatttgcgt agacggtaca ttaaagctgc
     7261 tgatggcgta tttcatatgc agcagtttat cgtgataacg ccccggtgcg acccagccca
     7321 cgcgcaggcc aggtgcaata cttttactga acgagctgca caacagcact cgcccgtcga
     7381 tatcccatga atgaatggtc cgcgggcgcg gatactccgt tgccagttcg ccgtagacat
     7441 catcttcaaa aatcacaata tcatgacgct gagcgagaga gagaacggcc cgtttgcgcg
     7501 cgtccggcat aataaatccc agcggattat tacagtttgg caccagaatg atgcctttaa
     7561 tcggccactg ttccagcgcc agttccagcg cttcaacgct gatgccagtt tctggatcgg
     7621 ttgggatttc aatcactttc acgcccatgc cgcgcagcat ctgcatcgaa ccgtaataac
     7681 agggagattc gaccgcgaca atatcgcccg gtttacacac cgccattaac gccagcgaca
     7741 tcgagttatg gcagccgctg gtgatgatga tgtcatcggc ggtgaccacc gagccgctgt
     7801 cgagcatcag gcgggcaatc tgctctcgca atacccgctg accggctaac aagtcataac
     7861 cgagaacggt ttgcagatta tgctgcacca cccggcttag ctcacgccac aacggtttca
     7921 ggctgggcgt ttcgacatcc ggcgtgcttt tgcttaacgg aacaatggaa ctgtcgctat
     7981 gcgcctccag catatccagc acctgatccc actgggtaat ttccaccggg cgctgcaccg
     8041 gacgcgtcat cggcggcact ggcggctggg ctttacgttg tgcgacaaaa taccccgaac
     8101 gcggctgcgg agtgatgagc ttcatcgtct ccagcgtctg atacgcctgc tgcacggtac
     8161 tgatgctgac gccgtgctcc tgacttaagc tgcgcaccga cggcaatttc tccccgtgac
     8221 gatacagccc ctgctcaatc cgctcggcaa gtagagtcgc cagatgttga taacgcgtca
     8281 tgctgtattc cttatttaga ccatacagag agaaaaaccg gtacagatga ggctaaaaaa
     8341 ccggatgcag atgctgtttt aacctgtctg tatgttaaat aaaactaata tttgcatctg
     8401 tattgtgaac tgccagaagg cggatgatga agcctctgaa agatgaggag gcagcagatg
     8461 gaatttcaca aaaacaaagc caaaacgccg tttatcagac ttgtgctact ctggcatgcg
     8521 atgaggcgtt ggcggcggca aatgcagacc cgacgagtgt tacagcagat gagtgatgag
     8581 cggttgaggg atattgggtt acgcagggag
//
LOCUS       DAZGCQ010000175         8511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000175 DAZGCQ010000000
VERSION     DAZGCQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.175"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK07_004764"
     CDS             complement(<1..242)
                     /locus_tag="ACHK07_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO8104985.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLE"
     gene            complement(354..1301)
                     /locus_tag="ACHK07_004765"
     CDS             complement(354..1301)
                     /locus_tag="ACHK07_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO8104986.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRQGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            complement(1385..1699)
                     /locus_tag="ACHK07_004766"
     CDS             complement(1385..1699)
                     /locus_tag="ACHK07_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104987.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKAELWYYNLFINPSADELMDVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            complement(1703..2137)
                     /locus_tag="ACHK07_004767"
     CDS             complement(1703..2137)
                     /locus_tag="ACHK07_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO8104988.1"
                     /translation="MNDNALVDVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKEGLQSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            complement(2247..3224)
                     /locus_tag="ACHK07_004768"
     CDS             complement(2247..3224)
                     /locus_tag="ACHK07_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO8104989.1"
                     /translation="MKTKKHTSRTATKTPEHDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMETVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSSDALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRKSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            complement(3317..3700)
                     /locus_tag="ACHK07_004769"
     CDS             complement(3317..3700)
                     /locus_tag="ACHK07_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104990.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(3816..4445)
                     /locus_tag="ACHK07_004770"
     CDS             complement(3816..4445)
                     /locus_tag="ACHK07_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104991.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(4527..4904)
                     /locus_tag="ACHK07_004771"
     CDS             complement(4527..4904)
                     /locus_tag="ACHK07_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104992.1"
                     /translation="MYSPLIAHYSALQTQSALLADISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(5100..5579)
                     /locus_tag="ACHK07_004772"
     CDS             complement(5100..5579)
                     /locus_tag="ACHK07_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104993.1"
                     /translation="MHSLLILLYVVVWRACIILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(5662..6054)
                     /locus_tag="ACHK07_004773"
     CDS             complement(5662..6054)
                     /locus_tag="ACHK07_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104994.1"
                     /translation="MSTFILCPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
     gene            complement(6520..6843)
                     /locus_tag="ACHK07_004774"
     CDS             complement(6520..6843)
                     /locus_tag="ACHK07_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104995.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(6876..7778)
                     /locus_tag="ACHK07_004775"
     CDS             complement(6876..7778)
                     /locus_tag="ACHK07_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO8104996.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRNNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGRDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            complement(8310..>8511)
                     /locus_tag="ACHK07_004776"
     CDS             complement(8310..>8511)
                     /locus_tag="ACHK07_004776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8104997.1"
                     /translation="TAPFITGNTQQWLCDPYPSGDAGMFLLRNGGIDFFYSFHLFAST
                     SCDCARCIRPFEIWSLTRVSGV"
BASE COUNT         2216 a         2404 c         1876 g         2015 t
ORIGIN      
        1 tgctccagtc cctcaatgac atgagcaagc aaatgcgcaa tgtggataac atcctgcttt
       61 tcataaccac tgacgatccg cagatacttc tgttcccggg cctcactgaa ctgaagccgg
      121 ttttccaggg caatcacact acagctgaca aagtcttcaa acaccttatg acgtttgtga
      181 taacaggctg tccggttaaa caacgaaatg aactccttct gatggtcagt catggtggcc
      241 atgttttctc cgtaaaaaca gggaacacat ggcccggaca ggggaacagg tgttcccctt
      301 cgggttgaaa tgttgataaa acgggcatca cggacatgat acccggaggt taattactgg
      361 gctgagacca gaggaaccgg taaaggtgcg gctttcgtct gttcttcagc gcactcctcc
      421 accaggtaca gtcggggctg tggaagtgtt tccctgcccg gccattcggc ctgcagatca
      481 ctgtccgtat gctctccgcc atcgtaacag ttgtaaccct gtataccgtg taccggctcg
      541 ctgtaccagt gtcggacctc gcagtcaaac accttcgaga tctcaccaac cagttcgcct
      601 gaaggcggat accagggcgt gtcgatcagc agcaccagac tgctcatctc atcacgcgca
      661 tggcgaacat tatttcctga cggccactcc acaccgtata tccggctgta aaggctggtc
      721 gttgtggaaa taccctgcag cagtccacct gaaccattca gctctgtagc cagacgggag
      781 ggtatgacca tcagcatgtc acagggctgc gcctgttccg ggtaatccga caaccagctc
      841 cagcaggctt ctgcatcaat gtgttcactc agcccggcca gaccaaacca gtcagaatac
      901 tgacggctca tcagcgtggt gatgatctcc ctggcattat ccggcagatt accccagcga
      961 acagcgccga tacatgactg gtgataaaga cgctcaatgg tctttatcgt ttcaccatcc
     1021 agaacggcat tttttaccag caaccccagc cactgctcaa acgcctggtt agccggagat
     1081 gacaaccccg ttccttgacg aaccagcgcc ggatatggcg gatactgcgt cgtccgtgtg
     1141 ggttttacca gccctgcaca accggccaga aacagctgta tactctgctg tatggcatga
     1201 cggtagcgcg gggtctcacg cccttcaatc cactgtgtaa gtacatcaag acagactgat
     1261 ttaccggtaa tttcaaaacg gtttttgcac cattcagaca ttgtttacgc tcctcttttt
     1321 tatacagatg taaaaagagg gcattgtccc gtgaggtaca atgccctcac gggaaaacgg
     1381 ggtatcacgc cccgttgttg cggataaaac cctgcagcca ggcagttcgc tcagggccat
     1441 accgcctgtt gacatccatc agttcgtctg cagacggatt gatgaaaagg ttgtagtacc
     1501 agagctctgc ctttaaatcc gccaggttcc ccctgccggt cctgagccac tcctgtacaa
     1561 catccctgat ttcagatgtg acatctccac ggtaatttga cgtgatcgta acatctgcca
     1621 gtaattcact gatatttcct gctatcagac acggttcaga gatcccgtta cagaaccaga
     1681 caaggcattt accagccata ccttacctcc tgtatttgct ggtatttatc ccgatctcgt
     1741 ttatggctac ggatatgttg cccggaaaac gcgctgtttc aatcttctcc agctcgtttt
     1801 tccggtaacg ctccacggtc acactcaacg ggacgttccc ctgtccgtac tctttacgaa
     1861 ggcgatcaat acactcctgt accagttcat cgctgaaata acgggattca agcacagact
     1921 gcagcccctc tttattctca aacccctctg tgacagagcg tacttcggcc tccgttggac
     1981 catccagcca gctcacccac acattgttga aaactttcgg gaaagatgga tcgcatttcc
     2041 gcacagaaaa tttctgtccc ggaaatgtat acgccagcac atccctgata cgggccacaa
     2101 cagcatcgtc aacatcaacc agtgcgttat cgttcatcac tcagttcctc tgtaatcctg
     2161 aaataacaga gagcactgcc ccgtgaggga caatgccctc acgggataaa tggcggtcag
     2221 gttataaccg ccaccggtag taaaaattat gctgcgcact cctgcgaagt tcgccgcgtc
     2281 agtaaatact ctgatgcttc acgtgcctgc cggcaggcgc gaaacagcgc ttttttgtca
     2341 gacttcagaa ccttaagcca tccgtcaata taactttcgt gctgtacgtc accggcaatc
     2401 ccgagatgtg cacacagaaa agcactgccc atctcggcaa tcagctcttc aaacgcatat
     2461 tccgggcttt tgcgaccggt aaaggtactt atccccaccc gccgcaggcg cttttcatga
     2521 ccggtactgt gtaccagctc atgcagcagg gtggtccagt aatcagccac ctggtcaaac
     2581 tgcgacgaca gtggcatcac gatacggtca agatcaggcc ggtagaatgc cctgtcctgt
     2641 ggaagtgacg agacgctgac gccgctgcca ttcagtacct gcagcacccg ctcactgaag
     2701 gtatctgccg gcagtgcatc cgagctgcct tccgccttca ccatcagatg ttcaggtaaa
     2761 ccgtcacact gccccacatt aaacagagga ttcgatttca gtaccggtac ggtttccatg
     2821 agaggctccc cctcactgtc cgtcagccgg ttaccgtgtt catccacagc ctgttttttc
     2881 cagtctttgt aaatcaccgc cagcgtggcc acctcccctt tacggacgtg gccacccagt
     2941 tgttgcgcct gacggtacgt cagccagtca ttgctgacat acccggcttc atccgcggcc
     3001 agccacagca acaggacgtt cacccctgta taaggcttcc cggtcagggc atttgccggc
     3061 atcatctccg gaagccccac gccattacgc gcagagcgcc agggacgttt ccagggtggc
     3121 acaccatttt caatggccgt aacaaccctg tcggtcacaa gctgatacag gtcatgttcc
     3181 ggagttttag tcgcggtacg tgaagtatgt tttttcgttt tcataagagc atgccccctg
     3241 aaaaagatgc acgctccctg tacggtgaga gtgcaccgta cagttaaatt aataacgaaa
     3301 atcagactgg ccagaattac cacaacgcca gcatcaccag tgaacgacgc tcaaaatcaa
     3361 acgtcttcag cgtattgctg acccacgctt ttacgtcatc cacgtcatac cagtaatcac
     3421 cggcataatc ttccccccgt aaacacgagc ctgtgacata aggaaacagc ctgtggcagt
     3481 tccccggtgt cagcatactg aggtcgcgca ataatgcagc cagtgaactg cgggttacag
     3541 ggaaagcatg ctgctcatct gaagcaccgg tatgttcacg aaaccatgtc agaagcggtt
     3601 tcaccttacg gaaagccccc acctcctgat gttcctgtgt tcccacaacg gcaccactgt
     3661 gatggttatc acgagccaga aagtaaatat cgagtcccat aacaattctc ctgaataaag
     3721 ccgggactct ccccacaggg atatgtccct gccgggtgat ataatgacgg aatacgtgat
     3781 gacgttattc ctgataaaga aactacggcg ttctgttacc gcccaccggc aagagcccgc
     3841 agccagtaaa gctgatattc ggcctcttcc agtgctgccc agtcatccgg ggataactgt
     3901 cgcccctgac cggacaaaaa ctgcagcagt aagctgtcaa acgcatctcc ccttaccggg
     3961 gtggttaata cagactccgg agcagaaaaa ttctcatgtc ccaggtaccg ttttatccat
     4021 ttcagcggca gcgaccagct ggcaccaccg acagtggaat gaatacgaaa acagtccgga
     4081 cacagattgt gcaacagcac aaagactgta cgggcaaaga aatctcccgg ctgacgtccg
     4141 aaattcagct cattaagagc caggatattc cggctgtgct cccgctcaat agtgacattc
     4201 accgaccaca gcccgtcggc agaccggcag taaaacgcca gcgtgtccgt taccggaaac
     4261 aactctgacg cccgggtcag ggagccgaaa tacgcattct gcggccgtaa cgcctccggc
     4321 aactgccaga aggtttcagt gacagccaga acaaaagcat gccactgctt ttccggtatt
     4381 gtggaaacct gctcaagggt atataccacc ggctcacggg ccggacgtga agataactca
     4441 gacatggaac attcctcatg gttaaaacga aacaacgggg aatgtctccc ggacggagaa
     4501 ctcccccgtc agggtcaggt catgaattac atcatgtcgt gccgggcaaa atattcatcc
     4561 gctccggcca ccatctttcc ggtactgata ctgtcagccc agatttcatg attaatttca
     4621 cgccccagag ccatcagact gtcgagtgcc tgacgggcct gaattcccgg tgcggaaatc
     4681 tcctcctgct ccaggtcata caacagccag cccagcgcct cgccggtatc ccacagatac
     4741 acgtatacca gtgaaggctc cgtctcatca tccggctccg gggtaatacc cagccgttgc
     4801 caggcacgta accgcatcag ctcgccttcc atctgactga tatcagccag cagagcagac
     4861 tgcgtctgaa gcgctgaata atgagcaatt aaaggtgaat acatgatgac ctccgtctct
     4921 taaaggaagt cctgtccctg acggaacaaa actcccgtca gggaaaacac cggcgcaaaa
     4981 acacggtgca gtaagtaaaa gaaaaaccgt tacgggagca aaacagccat cattgcaaaa
     5041 tggcagttgc atcgctcacg atttgacatc cttcctgtga ctgactggca tcctgcccgt
     5101 cagtctgcgt cggaaaattc aatacaggcc tgcttcagga gcccggctat acgttgattc
     5161 agacattccg tccagccatc gttccatcca cgctcccatg gattgcagac atcctcccat
     5221 ggattgcaga catcgttaca gccattcttc catgtcttcc atgcctccat ctccatgaca
     5281 gcccccagcc tgtggccttt cacacgtata tgccacagaa acggccggaa aagtcctgat
     5341 ttcagttggt caccaccgct tttgtagccc agatgacgtg caatactttc agtatattca
     5401 aatgcatcat tctgcattcc agccgcctta gccagcgagc tgtacgcatc acgaagtatc
     5461 gtcatacctt ccagttgata ctcacaccac gcacggacag ccgacactgg tgcgattacc
     5521 cgaagaatga tgcaggcacg ccatacaaca acgtaaagga gtattaacag actatgcatg
     5581 tgttcacctc agatacaaaa aataacggga acacacctgc cctgcgggaa gcgtgttccc
     5641 gacagggtaa taaatgataa atcagcaggg ctgtgcgacg attccatcac cctgcagacc
     5701 aaaccggtaa cgcagggtgg tcagttgctc cgccatacgg caggcagcat gccatgtcct
     5761 gcgacgggat atccgtccgg taaaccggtt cagataaatg acctcacgcg gtaacggctg
     5821 ataccatccc gtgtactcgt tgcgatacat tccctgccgg taatacaggc agggtgtcca
     5881 gacaccgcca cgacggaaca tacagacccg cacgctcaga ttgccggccg gattagtgta
     5941 ccagcgactg taatatgaca ggttattgcg ggaaaaaaac acccgcaact gagccgtcat
     6001 tcgccggaaa aaagaagaga tgcccggttc agggcacaaa ataaacgttg acataaagac
     6061 tcctgtaaaa aaacgggagc cttcgccccg acggacaaaa actcccgtca gggttaaaac
     6121 accaccagta cagcatggtg ttgctgtcag aaaaagcccg ttaataaagg gcgcagttat
     6181 aaagaaacct tcatcgtcat gactgacgat atctgctctc aaaggtgggc agatttacca
     6241 ccaccagaaa catcctggct gcacatcccc tttcaggtta cgggggtgtt gatcacccga
     6301 aggcgttcct ctcagatcaa cggaacattg gtgcccaccg aagcgggatg tggtcacccg
     6361 aaggcgctcc tctcagacca cttatggagc attggtggtc gccgaagcga catgtagcca
     6421 cccgaaggcg ctcctctcag gccacttgtg gagcattggt ggtcgccgga gcgacatata
     6481 agcctgtata tttttaaatc acaaaagatg aatgtcaagt tattcaacat caatccaaag
     6541 aggaatatta tgtattccct taacgggttc tcccagagga aaaacacagg tccctttaat
     6601 cagtattttt cttcccgacc atctgacatc acagttaaaa tctcccacca catcaaaata
     6661 atccgcaatg gattcataga tgtcccattc atcatagata tcccatttat ctgctgttcc
     6721 ttcacattca aactcggcct gatatccgga atcaacatat cgcgccagat agtgtgacca
     6781 caaaggcgaa ttttttgtaa tctcttttac agagattatt ttcagcaaaa tagtacaatc
     6841 cataatattt acccgtacga aatcgccatg agttattact catagcctaa caactgacaa
     6901 taaacctgtt caatatatcg tcctctgcca tcaccatgac caagatcaat tgatgtcagt
     6961 gcaagtgcct ccttataact gtaaccgttc ataatatgat atttcatggc acttgcagcg
     7021 taactgtacc gaaaactgtg tgcagtctca tctctgcctt tcagttctcc ataacgacgt
     7081 aatgtattaa gataataatc cattgccgac ttaagattat ctctgtctat aagacgtcca
     7141 ttatgttcac tggcatattt gagtgcataa ttaacagcat caagaacgtt atcccggtca
     7201 acaaccgttg tcattctttc ccgtccccct ttcgtaccaa aaatcactct tattttttca
     7261 tctccccgtt gtaacgcttt tttccaggtt cgtaatgatt tggccgactg gatagcttct
     7321 tcatttctca gaccgaggta acgtgacagt ttaacaacag cagccacccc tttatctttt
     7381 gcttccacac gctgaagaat ctcccgaaaa cgctcatccg tgattaattt ttttgttccc
     7441 ttacgactgg agccggaaat gcctaacgct ttattgctga gacgaggatg ttcgggatca
     7501 gccatcttat atttacctgc ctgacgtaac ataacccgta ttgcagacat ttcattttcc
     7561 cttgttcgcc cagaaacatt attccggctt ctcatatata gctcaatatg ccggactttt
     7621 acctgcgcgg cagaactgat ctgtacattc atttttgcca gactttcagc aaagcggtgc
     7681 atgatcctgg tgtaatcact cagcttcttg aagctgcctt tgtcttttcg aatgtgagca
     7741 accagatcct gtttcagtaa tttttcacgt ctggacatca acttctcctg cacataataa
     7801 tttttacctt aaacagctgt acgttcggca gatgagtttt tctgttgtgc cgtgaacaca
     7861 acagaaaaac tcatctgcat ttctccctga cgacacggtt aaacgttgcg atccttcggg
     7921 tagtgactgc ctgtgcgcca gggtagcgca gattgtttag gtattgaggg gtaacaaatc
     7981 ggtgttgcac accgactgca gcatttgctg tacctgtctc caggtcgccc ccgacaccac
     8041 ctctaataag tggtatcgcc gattcggcat ctcactacat aaaactcctt aaaaaaacaa
     8101 ttgattctgc aaaaaaccat ttatggacgt tattcacaaa atcgtccgta aatttgcaga
     8161 agaggttgaa aaaacactct cacgccgggt cacttcttca aatgttaaaa aacatcacgt
     8221 gaaacggcag ttcgtgcggg ttcgttaagc agacgcgcgt gcgtcacttt gtcatcgtga
     8281 cacgctgacg aaagtgacgc cgcgcagctt cagacgccag atacacgcgt aagcgaccaa
     8341 atttcaaatg gtcttataca gcgcgcacag tcgcaggaag tgctggcaaa caaatgaaaa
     8401 gaataaaaga agtcaatacc gccattacgc agtaagaaca ttccggcatc accggatgga
     8461 taaggatcac acaaccactg ctgtgtattg cccgtaataa agggcgcggt t
//
LOCUS       DAZGCQ010000176         8448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000176 DAZGCQ010000000
VERSION     DAZGCQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.176"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..1514
                     /gene="adeQ"
                     /locus_tag="ACHK07_004777"
                     /gene_synonym="yicO"
     CDS             180..1514
                     /gene="adeQ"
                     /locus_tag="ACHK07_004777"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO8104998.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1567..2019
                     /gene="yicN"
                     /locus_tag="ACHK07_004778"
     CDS             1567..2019
                     /gene="yicN"
                     /locus_tag="ACHK07_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO8104999.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2135..2458)
                     /locus_tag="ACHK07_004779"
     CDS             complement(2135..2458)
                     /locus_tag="ACHK07_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8105000.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(2442..2795)
                     /locus_tag="ACHK07_004780"
     CDS             complement(2442..2795)
                     /locus_tag="ACHK07_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8105001.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            3029..4213
                     /gene="nepI"
                     /locus_tag="ACHK07_004781"
     CDS             3029..4213
                     /gene="nepI"
                     /locus_tag="ACHK07_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO8105002.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLCGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4415..>8448
                     /gene="air"
                     /locus_tag="ACHK07_004782"
     CDS             4415..>8448
                     /gene="air"
                     /locus_tag="ACHK07_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HFO8105003.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTTTSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDYDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEEPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNDNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGTAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVRTAQIADLVVIKDGSVADGSTANTLRVRVSDAFGNALDGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLVVMQ
                     DGSVADGTTANTLRARVTDAFGNALAGQTVSVTAGNSATVASTVTTKPDGTVEISVTS
                     QTAGVSTVTASINSSSQSRDVTFVA"
BASE COUNT         1984 a         1986 c         2282 g         2196 t
ORIGIN      
        1 ttcatatttt tctcattttg attttatcaa tttgattatt atttatcata aagaaacgtc
       61 gcgagattat taaagaatat ccattaatgt gcaattgaaa tgtgataatt atcactgaac
      121 cgttgttgtc atttgtttta caaaagcaag ggattaaatc tcctccaatg gacaaaaaaa
      181 tgaataatga caataccgat tacgtgagta atgaatcagg gacgctttcg cgattattta
      241 aactacctca gcatgggacc accgtccgca cagaattgat tgcggggatg accacttttt
      301 taaccatggt gtacatcgtt tttgtgaacc cgcaaatcct cggcgcggca caaatggacc
      361 cgaaagtggt gtttgttacc acctgtttga ttgccggtat cggcagtatt gcgatgggga
      421 tatttgctaa cttacccgtg gcgctggctc cggcaatggg gctgaacgcc ttctttgcct
      481 tcgtggtcgt gggggctatg gggatctcct ggcaaaccgg gatgggcgcg atattctggg
      541 gtgcaattgg gctatttttg ctcacgctgt ttcgtatccg gtactggatg atctccaaca
      601 ttccattaag tttacgtatt ggtatcacca gtggaattgg attatttatc gccttaatgg
      661 gattaaaaaa tactggcgtt attgtcgcca ataaagacac cctggtgatg attggcgatt
      721 taagttctca cggtgtgttg ttaggtattt tagggttttt tattataacc gtgttgtcat
      781 cacgtcattt tcatgccgcg gtgctggttt ctattgtggt gacgtcttgc tgtggattat
      841 ttttcggtga tgttcatttt agcggcgtct attccattcc gcctgatatt agcggcgtca
      901 ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc attttctcct
      961 ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact gataaagctg
     1021 gcttaataga tagtaacggt aaattcccca atatgaataa ggcgctgtat gttgatagcg
     1081 tcagttcggt ggcgggcgcg tttatcggta cctcgtctgt tactgcctat attgaaagta
     1141 cttctggtgt ggcagtcggt ggtcgcacgg ggctaacggc tgtcgtggtc ggcgttatgt
     1201 tcctgttagt tatgttcttc tcaccgctgg tggcgatggt tcctccttac gcaaccgccg
     1261 gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg
     1321 attttaccga atcagtgccc gcatttatta ccacggtgat gatgcccttt actttctcga
     1381 tcaccgaagg gattgcactc ggctttatgt cgtactgcat catgaaagta tgcaccgggc
     1441 gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca ctgaagatta
     1501 ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga ttacgcagga
     1561 gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt tggttttcgg
     1621 gtgctgacat ccggggccag aaaagcgatt cgccgtctca gcgatcggct gaatatcgat
     1681 gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagctggtga cgaattttta
     1741 cgttatttgc atcgtccgga tgagtcgcac ctgcaaaacg ccgcgcaggt gttgctcatc
     1801 tggcaaattg tcattgtcga tggtagcgaa cagaacctgc tgcaatggca tcggatttta
     1861 caaaaagccc gccttgccgc gccgattacc gatgctcagg tcaggctggc gctgggtttt
     1921 ctgcgcgaaa ctgaacctga aatgcaggat attaatgctt ttcagatgcg ctataacgca
     1981 ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt cttatcagcc
     2041 tacagaccgt gcctgacctg tcgagtggat aaggcgttca cgccgcgtcc ggtatcggaa
     2101 ctgacagtag gtgaacgcat tatctacgtt ctatttacag cagcacatcc agcccgtgtt
     2161 tttgcacaat gctgagcagt tttaatgaaa tgccattggg gcgtttagcg ccactttccc
     2221 atttttgtac agttgaaacg ctggtattga gatagtgagc gaagacgggc tgactcactt
     2281 ttaatttttc ccgcagtgct tttatctcaa caggttgtaa gtcttgcacg ctggtcaggc
     2341 aggctttgtc aaaagtacgc attgtttcgg cagaaatagc atcgacgctg gacaatccag
     2401 ccgctgcgct gtgaatagcc tcaaatgcag gacttttaaa tttagttttt gcagtcatag
     2461 cagatctctg tcagttcttt agtgttaatc attgctgtta gctgggcttt cgtcaggaag
     2521 gcataatgct ttgcaagttc acgaaaccca gccagttcct gactattgat gttcgacata
     2581 tcctgttttg catataaaaa cgtgtaaaac caattgcttc ccccttttgc cagaataatt
     2641 gcacgctcac gattttggtg aaggcgcttt ttaaaaacgc caccaccaag tgcatcagct
     2701 ttaccttgct ccgtttctaa aatggcccgg cacaactctt catctgttat tgcatgagac
     2761 ctggcttccc ttgcaaacca tttggtttta aacacgcgca tatcgttgag gtcctgttat
     2821 taaagtatat caccaagtgc aatactttat cccttattcc caacccaatc gcgaaaatgc
     2881 atatcagctt gccctatgat ttttgtttct tcgagcagcc tcgaactttt cccacaacac
     2941 cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca atgttaaagt ggtcggcttt
     3001 tcccctgaaa catgccacgg gtaacacaat gagtgaattt attgctgaaa accgcggcgc
     3061 ggatgccatc acccgaccga actggtcagc cgttttctcg gtggcgtttt gtgtcgcctg
     3121 tctgattatc gttgagtttt tgcccgtcag tttgttgacg ccaatggccc aggatttagg
     3181 catttcggaa ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc
     3241 cagtttgttt attacccaga caattcaggc cactgaccgc cgctacgttg ttattttatt
     3301 tgccgttttg ctgacgctct cctgtttgct ggtttccttt gctaactcat tcagtttgct
     3361 tttaatcggt cgtgcctgtc tggggctggc gctgggcggg ttctgggcga tgtcggcgtc
     3421 gctgaccatg cgtctggtac cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg
     3481 cgcggtttct attgcgctgg tgattgccgc gccgttgggc agttttttag gcgagcttat
     3541 cggttggcgc aatgtcttta atgcggcggc ggtgatgggc gtgctgtgta ttttctggat
     3601 tatcaaatca ttgccttcac tgccaggcga accctcgcat cagaaacaaa atactttccg
     3661 cttattacaa cgtccgggtg tgatggcagg gatgatcgcc atcttcatgt ctttcgccgg
     3721 acagtttgct ttcttcacat atattcgtcc agtgtatatg aacctggcgg gattcggtgt
     3781 ggatggctta acgctggtgc tgttaagttt tggtatcgcc agctttatcg gtacgtcgct
     3841 ttcgtcgttc attcttaaac gttcggtaaa actggcctta gcaggcgcgc cgttaatact
     3901 ggctgtgagt gcgttggtgc tgacgttgtg tggaagcgat aaaatcgttg ctaccggcgt
     3961 ggcgattatc tgggggctaa cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac
     4021 ccgctcactg gccgatcagg cagaaaaagc cgggtctatt caggtggcgg ttattcagct
     4081 tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg gataatattg gtctgacttc
     4141 gccgttgatg ttgtccggca cattgatgtt gctgacggcg ttattagtca ccgcaaaggt
     4201 gaaaatgaag taacgtattt acaggagggt tatttctctg gcgggaaagc tatcctgttc
     4261 ttccccctga taacgcttga tcaggtattg ttcttatctt gttgttattt ccattattcc
     4321 gcctgtattc atcatttgct aatcatgaat catcattttc cctcatgtta aagaaaggtc
     4381 atttaatctg aacgcatagc gacagggatg acaaatggca gggaaagcgc atgggaatgg
     4441 cgatcgtcgg ggcgataaca ccatatgtgg attgggggat cgtttacgtc gtctgaccgc
     4501 cggtatttgc ctgataacac aaactatatt ccctgttatg gccgctgccc ctacgcatat
     4561 taatcatgcg cattcggata ctaccacgtc cctgatcctg ccgaacgtta aaactattcc
     4621 atataccctg ggggcattgg aatcgccatc aacggtcgcg gcacgctttg gtatcaccgt
     4681 tgatgagtta cgtcgcctga accagtttcg tacctttgcg cggggctttg acaatgtgcg
     4741 tcagggcgat gaaatagatg tgcccctcat taacagtaat agccctgaag cgcgcaacct
     4801 gaaagcaatg cagatggagc gcgacggcaa agatccgcaa atgcaggtcg cggaaatggc
     4861 gcagcaaagc ggaacacttt tagctcgcga tatggacagc gaacaggcgg cgagtatggc
     4921 gcgtggctgg gtggcttctt ctgcatctgc gcaggcaacg gactggctca gtcgttgggg
     4981 aactgcccgg gtatctcttg gtgtggatga agatttcagc cttaaaagct cttcattcga
     5041 atttctccat ccctggtacg aaacgccgga taacctggtc ttcagccagc acacacttca
     5101 tcgtacggat gatcgcactc aaaccaacca cggtatcggc tggcgttatt tcaccccgag
     5161 ctggatgtct ggcgtcaata tgtttatcga ttatgacctg acccgctatc acacccgaac
     5221 cgggatgggc gttgaatact ggcgcgatta cctgaaactg agcggcaacg gttatctgcg
     5281 cctgagtaac tggcgtagtg caccggaact cgaccatgac tatgaagcac gtccggcgaa
     5341 cgggtgggat cttcgcgcgg aaggctggtt accggcctgg ccgcagctgg gcggcaagtt
     5401 ggtctatgaa caatattatg gcgatgaagt ggcgctgttt ggtaaagacg agcgacaaaa
     5461 tgatcctcac gcgattaccg caggtttgag ttacacccct gttcctctga tctcctttag
     5521 tgccgagcaa cggcagggca aacagggaga aaacgacacc cgaattggta tggagttgac
     5581 gttgcaacct ggtcattcac tgcaaaaaca gctcgatccg gctgaagttg cagcacgacg
     5641 cagccttgtg ggcagtcgct atgacctggt cgatcgtaat aacaatattg tcctcgaata
     5701 tcgcaagaaa gagctggtgc ggctgacgct gaccgatccg ttgaaaggca agccgggtga
     5761 ggttaaatcg ctggtttctt ccttacaaac caaatatgcg ctgaaaggtt atgacattga
     5821 agccgcctcc ctgcaatcag cgggcggaaa agtagctgtt tccggcaagg atattcaggt
     5881 cactattcct ccctaccgat tcacggcaat gcctgaaacc gacaacactt acccgatagc
     5941 ggttacagcg gaagattcga aaggaaattt ctcccggcgt gaagaaagta tggtggtcgt
     6001 ggaagaaccc acactaagcc tcgccgattc aacgctttct gtcgatctgc aaatcctgct
     6061 tgctgatgga aaatcaacct caacgctgac ctataccgcg cgagattcca gcggcaagcc
     6121 aatccccggt atgacgttaa aaacacaggc gaaaggtttg caggatttcg ccttaagcga
     6181 atggaaagac aacggtaacg gaacctatac ccaaatagtt accgccggga agacgtctgg
     6241 cgcgttatcg ctgatgccgc aatttaacgg cgacgatatc gctaagacgc cagcgctgat
     6301 agccattgtg gcgaatacgg catctcgtgc tgactccacg attgagacag accaggataa
     6361 ttacgtcgcg ggtaagccga tagtcgttaa agtgaccctg agagatgata acgacaacgg
     6421 ggttaccgga cgaaaagaac ttctcaaaca aaccgttaaa gtagacaaca cgaaggctga
     6481 tgccgttagt gcctggacgg aagaaagtga gggaatttac aaagcaagct atacggccca
     6541 tctcattggc gacaaattaa cggcccagct gacgatgccc ggctggcaaa ccaaacattc
     6601 ggatgcgttc agtattgcgg gtgataaaga taccgccaaa atcgctgcga tgcagatcac
     6661 cgcgaataac gcagttgcca gacgcgatca caataccgtc gctgtgacgg tcagggatgt
     6721 gcatcaaaat ttactgcagg gacaaaatgt taccttcacg gtcgtgaatg gtacggcggt
     6781 ttttgccgat ccgaatggcg gtattgtgac gacagataaa gacgggatag ccagcgttaa
     6841 ccttgccagt gaccaggcgg taaacagtct tatcaaggcc gagatcaatg gcagtagtca
     6901 gtctgtggaa gtgagcttta tcactggcga tatctcgcag ctgacctcca ccatcaagac
     6961 ggacgatgtg tcatacactg cgggtggcaa gataaaagtg tccgtcaccc ttatggatga
     7021 gcagaaaaac ctggtgaaag ggatggcttc gctgctggcg ggtagcggtg tggtcgaggt
     7081 gagcggtaca gataaaaatg aaacgggtaa ctggagcgaa gagtctgacg gcgtttacac
     7141 caccacccga accgcgaaga ttgctggcga tcgccattac gccacgctga aactatcgac
     7201 atggagtagc gcgcagcaat ccgatgcgta tgccatccgc gaaagcggag ctgtgctggc
     7261 ttattcctcc atcgttaccg acaaaacgac ctacaccgcg ggcggcgtga taaaagttac
     7321 ggtaacactc aaggatagct atgaaaacct ggtcgggggg caacgagacg ctatcaacca
     7381 ggctattcag ctgccaaata caaaagcgga aagcatcgcc tggaatgaag atcaaaaagg
     7441 aatttatacc gcaacgtata ccgccttact ccctggcacc ggtttaaaag cacagctaca
     7501 gatgtccgac tgggcgagcg ctctgacttc aaatgattac agtattagtg gcgatgccgc
     7561 cagcgcacaa attgtcgcga tgcaagtgac aacgggtaac ccggatgtgc tggcgaatgg
     7621 cagcgatcga catacggtga atgtccgggt ggaagatcag tttggcaatg tgctgccgga
     7681 gcagaccgtc acctttacgg tgacgaaggg ggcggctgta tttgccaatg cagggcagag
     7741 cgcagatatc aggacggatg cgcatggtat ggcagaggtt gatctgagca gtaccgtagc
     7801 ggatgccagc actgttgagg ctaaggtcaa tcaaagcagc gatagtaaaa cggtgaactt
     7861 tgttgctgat gttaggacgg cgcagatcgc tgatctggtg gtcattaagg atggctcagt
     7921 ggcagacggt tcgacggcga atacgctgcg ggtgagggtg tcggatgcgt tcggtaatgc
     7981 gctggacggg cagacggtca gcgtgttggc ggacaatggc gcaacggtcg ctccgacggt
     8041 tatcaccgag ccggacggga cggtggagat ctctgttacc agccagacgg cgggagtcag
     8101 tgcggtcacc gccagtatca acaacagcag cctgagccgg aacgtgacgt ttgttgccga
     8161 tgtcaggacg gcgaagatcg ctgatctggt ggtgatgcag gacggctcag tggcagatgg
     8221 cacgacggcg aacacgctgc gggcgagggt gaccgatgcg ttcggcaatg cgcttgccgg
     8281 gcagacggtc agcgtgactg caggcaacag cgcaacggtc gcttcgacag tgaccacaaa
     8341 gccggacggc acggtggaga tcagtgtcac cagccagacg gcgggagtca gtactgtcac
     8401 cgccagcatc aacagcagca gtcagagccg ggatgtgacg tttgtcgc
//
LOCUS       DAZGCQ010000177         8350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000177 DAZGCQ010000000
VERSION     DAZGCQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.177"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..233)
                     /locus_tag="ACHK07_004783"
     CDS             complement(<1..233)
                     /locus_tag="ACHK07_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO8105004.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGV"
     gene            complement(246..533)
                     /locus_tag="ACHK07_004784"
     CDS             complement(246..533)
                     /locus_tag="ACHK07_004784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8105005.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            complement(549..992)
                     /locus_tag="ACHK07_004785"
     CDS             complement(549..992)
                     /locus_tag="ACHK07_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8105006.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            1263..2294
                     /locus_tag="ACHK07_004786"
     CDS             1263..2294
                     /locus_tag="ACHK07_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8105007.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            2691..2960
                     /locus_tag="ACHK07_004787"
                     /pseudo
     CDS             2691..2960
                     /locus_tag="ACHK07_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3191..3289)
                     /locus_tag="ACHK07_004788"
                     /pseudo
     CDS             complement(3191..3289)
                     /locus_tag="ACHK07_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      3410..3598
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3663..5507
                     /gene="btuB"
                     /locus_tag="ACHK07_004789"
     CDS             3663..5507
                     /gene="btuB"
                     /locus_tag="ACHK07_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO8105008.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            5521..5712
                     /locus_tag="ACHK07_004790"
                     /pseudo
     CDS             5521..5712
                     /locus_tag="ACHK07_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5853..7943)
                     /gene="iha"
                     /locus_tag="ACHK07_004791"
     CDS             complement(5853..7943)
                     /gene="iha"
                     /locus_tag="ACHK07_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO8105009.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            7882..8064
                     /locus_tag="ACHK07_004792"
     CDS             7882..8064
                     /locus_tag="ACHK07_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105010.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
BASE COUNT         2242 a         1976 c         1915 g         2217 t
ORIGIN      
        1 acaccctgaa taccaaaggc atgctggaaa atgtttgcga agtcacccag tgacgacaca
       61 actaaacccg cccctgcgcc taaaatcaca aaccccataa tggtcttgac ggtaccttta
      121 atgcattcgg ttaccggttt tttctgtgca atcaggccaa tcagggcgat taaaccgacc
      181 agcactgcgg gttcggaaag tacatcgctc atcaaaaaac gaaagaaatc catatcgccc
      241 ccagcttaca gtgcgcctaa ttcggttaat accacggaca aacgttctct catggctttc
      301 ttatcaatca tattttccag tgcgacgatt ttgcctgcga cagactgcgc ggctaactgc
      361 tcggcaatgt ctttggtgcc gacatagata tcactcggcg tccctttcgc ggagcccagg
      421 tcgacgtgat caacatcagc gctgacggac aaatccttca aaatgctttt aatgctcatt
      481 tccatcatca ggctggtgcc taagccgtta ccgcaaacca ctgtaatttt cataaatgtt
      541 cctctggatt aatagcgcgc aataacagac aaaatgtcct gcgtcgtttt ggccgtcaaa
      601 atggcctgta tgtctttttc gttatcgaat aacttggcga gttgagaaat cgcttcaatg
      661 tggctggtac tgtcggttgc agccagcaca atcagtaatt tcactggatc gttttcatcg
      721 gcatcaaaat tcacacctga agggatcagg gtcagtgcta atgacagctt gttagccccc
      781 tcttccgggc gcgcatgagg catcgcaata ccagggccaa ccacatagta cggaccgata
      841 gtgtcgtgcg agtgatagat cgcatcaaca taacgcggtt cgatagagcc gttttcaatc
      901 aacggtcggc atgaaatcgc cacggcttcc cgccagtctt tgacctgttc cacgacctga
      961 acgacctctg tatttaatag attttttaac atgctactga taactcccga tgataattaa
     1021 caggagagta gatattttac gcattaatta atgtgatgat tgtcgcattt gatagcgcta
     1081 tctgataacg ctaccaatct ttgtgatagc gctatcattt ctctgccgcc atgctttggc
     1141 tatattcggt tcctgcatat acaatgcaac aggatatttg ctaccgtggg caaaaactgg
     1201 aaagagtaat ggtgaaataa aagtaatgaa aacagctggt tgtaattatc catggagtcg
     1261 aaatggaaaa aacacgtaag cgccgtggta ccggacgggt gacgctgcag gaggtcgcta
     1321 actttgcagg tgtcggaacc atgaccgttt cccgcgcgct gagaacaccg gaacaggttt
     1381 ctgacaagct gagggaaaag atagaacagg ccgtagaaga gttgggttat attcccaaca
     1441 gaactgctgg cgcccttgca tctggtcaca gtcatacagt cgccgtactg gttccttccc
     1501 tgacagataa agccagctcc cgctttatgc agtcattaca gcaggtgctt aataaaaatg
     1561 aattccagct actgcttggg tgtcatgagt ataatcagct caaagaagct gaaattctga
     1621 tgacgcttct tcaaggcaac ccggcggcgt tagttatttt tggttctcag ttggctgata
     1681 aaactcacca gatactcgag aaaacaaata tacctaccat taacgttatc ggttcccctt
     1741 tcagtccggc tcaaattacc atcgaaacag cattttttga agcatcacat aaactgaccg
     1801 aacatctgct ggagcagggt tataagaata tcggatttat cggtgcgcat atggacaacc
     1861 gcctgcaacg tcagcaactg aacggctggc ataaagcaat gctgagccat taccagaaca
     1921 ctgatcttgt tatcaccatg ccggatgccg caagtctgca attggggcgt tacgcattaa
     1981 acgagatatt actgcgacaa ccagagctgg atgctgtgat ttgcagccac gaagagattg
     2041 cgctgggtat aatgtttgag tgccagcggc gtttgttaaa aatccctgga aatatcgctg
     2101 tagcttgtct tgacggttca gacaattgcg atcaaacaca cccaactttg acatccatcc
     2161 gtattgacta caaaaaaatg gggatagaga caggtaagtt actgattgga ctattgaata
     2221 ataatcatga tgaatcagaa gagtcgcgta tcgtgcaatt caactaccag atagaattac
     2281 gtcagagtac atgagcctaa aagagcgcta ttacaaagtt agctattgtg gtagggatag
     2341 ccgtttccgg ctattccctg cgcagttccg gatgtatgct gctaacgcat ccattcctac
     2401 ttcaggtatt tggcgtagaa gcggatgcta aaaacttgcc gcaaaaccgc aatatcagcc
     2461 tgcagtatcg ctctccagat tatccattcc tatattgatg acaacgacag gtaagaccgt
     2521 gacctgataa acctgaataa gctcctggac ataaagtccg taatgggaat aaggcgagag
     2581 tcagcgaatc tcatcgaggc cagcagcact ggctgcaata aggatataga gcagcaatct
     2641 accgtcaacg tcaaaaacac ggaagccttg caaacgggat gcgttcatat aaacaacatg
     2701 gagcagcaac aatacgatgc ctgtcgttgc tcagtcagga ccttttctgt catgcgcgag
     2761 ccagcgtcac gattgataaa taatgttagc aactgattac tttactgcaa cagacgtggt
     2821 tggtaataag cactggcgga caatgcagga tggaaggctg ttttcaggtt gtccgttcag
     2881 gtacgttaga tcagttcgtt gcgctaaaga caactgttca gtcccttatg ttacccgtta
     2941 tggcaaccat tgcccggtga taaccgggcg tgtcatctgt gcatccgttg attcgtgtgg
     3001 actgataaag ttttgtctgc tgaatcttta tacggaatgc tcttcagtat gcgtaagata
     3061 atttgttttt tggaggattg tcgacaatca gagggtaaac aaaagaatgg ttgaatgtgg
     3121 atgaggttca tattattgat tcagattttt tgaagaatca ccacatggga tgtccatgtg
     3181 gttgtataac tcagggccag cgcttaataa tgatggatgt attaatgccg ccaaaggcaa
     3241 agttgttact ctgcaaaaac acacaatcaa ccttgcgggc ttcatgcaac taattgagca
     3301 tatatgtttt attcttatta tggtcgccga taaaatatta tgtgtattag atccgagtgt
     3361 tcatgcattc ctatttttga gtcatgtatg gacagtaact tgttacaacc agtaatatcc
     3421 agttgtcggt cctgcgagtt aatagggaat ccagtgcaaa tccgaaactg acacgcagcg
     3481 gtaaggaaag gtgcgatgat tgcgtaatgt ggacacggtc atccggtggg tggtcatctc
     3541 tttgtatctt cagatgccct tctaggtctg aagacctgtc ggttaacgtc gcatctggtt
     3601 ctcatcatcg cttactatcg atggtaactg cgatattctt cttctgttgt ggatgcgtta
     3661 caatgattaa aaaatattgg tttctaatgg tgtactccgt cacagccttt tctgtctggg
     3721 cacaggatac ccctctggat actctcgttg ttactgctaa ccgttttcag gaaccattaa
     3781 gtaccgtgct tgctcctgta actattgtga cacgagaaga tatagatcgc tggcaagttt
     3841 cttctgtaaa tgatgttctg cgtcgtttgc ctggcgttgc aatttctcag catgggggag
     3901 aagggcagct ctcaacaatt tttgttcgtg ggacaaactc taaccacact ctggtattga
     3961 ttgatggagt gcgcctgaat ttagcgggtg tcagtggagc tgcagacctg agtcagtttc
     4021 ccgtcgcttt agtgcagcgt atagaatata tccgtgggcc acgttcagca atttatggtt
     4081 cagatgccat tggcggagtg ataaatatta ttacatcccg tgagaatact ggcactgaaa
     4141 tttccgcagg atggggaagt aatagttatc aacattacga tatatccact catcagcaac
     4201 ttggggaaaa tacccgtgta acacttcttg gcgattatac ctatactagg gggtttgatg
     4261 ctgttgccta tggcagtaca gggatgcaac cacagtcaga cagagacggt tttttaagta
     4321 aaacattcta tggcaaactt gaacataact tatctgatac gtggagcgga tttgttcgtg
     4381 gctacggcta taataaccga actaaatatg acgcctggta ctctcccggt tctccactga
     4441 ttgatactcg aaaactttat agtcaaagct gggatgcagg tcttcgctac gctggtgaaa
     4501 cactgcaatc tcaacttgtt tccagttata gccatagtaa agattacaac tacgatccac
     4561 attatggacg ctatgacaca tcagctaacc tggatgatat gaaacaatac aatctccagt
     4621 ggacaaatag cgtcacagtc ggccacggta atgttggcgc tggtatcgac tggcaaaaac
     4681 aatccactac tcctggaact ggatatctgc caaaaggtta cgatcagcgt aacacgggga
     4741 tttatctgac aggactgcaa aaacttggcg attttacgtt agaaggcgcg gtgcgtaatg
     4801 acgacaactc gcaatttgaa cgccacacaa catggcaatc cagtgcggga tgggagttta
     4861 ttgagggtta tcgctttatc gcttcctatg gaactgcttt caaagcgcca aatctggggc
     4921 agctatatgg tatgtatggt aatccgaatc tggctccgga aaagagtaaa cagtgggaag
     4981 gggcatttga aggtttaact gggggcgtaa attggcgtat ttcaggatac cgtaatgata
     5041 tcagtgaaat gattaactat aatcctcata cccttcgtta ttacaacgac ggaaaagtac
     5101 acgttaaagg aattgaggct acagtaaact ttgatacagg agcactgacg catactgtta
     5161 gttacgatta tacggatgcc agaaatgcct taactgacaa accattggaa cgtcgtccaa
     5221 aactgcaagt gaagtatcaa cttgactggc aagtattcga ttttgactgg gggataactt
     5281 atcaatatat gggatcgcga tacgattcgg actactcatc ctggccctat aagtcagtca
     5341 aaatgggcgg tgtgagttta tgggatgttg cggttgcgta tccggtcact cctcacctga
     5401 tagttcgcgg taaaatagct aatctgttca acaaagatta tgagactggt tatggctatc
     5461 aggcagcagg acgggaatat atattgtctg gcagctatac tttctgaaat ctgccccacc
     5521 gagtcgctaa gcagcgtgat ccgccatgcg atcaaaaagc gtaaagtgtt cccgacagac
     5581 gaggcggtga aaaaagttgt gtggctggca atccagtctg cgtcccagaa atggacgatg
     5641 ccgttgaagg actggcgtat ggaaatgagc cgctttatta tcaagttcag tgaccgcctg
     5701 gacggtcact tctgggaaaa ggcatttaca cagaatcctg aacaggctcg gtcacttcga
     5761 ccagacaccg tgcgtcatgt aacgttgcca ctgttccgcc aggagttgca gtggcaacgt
     5821 attctaccgt cagtgatagc gttttgttat tatcagaact gatagttcag cgacatccag
     5881 taatttcgct caggtatcac atatcccgtt gttgatgatc ccgtctggaa gtaatcaccg
     5941 gcatacagcg tactcttacc ggcactgaac aggctcacgt cactgtaatc cttgttgagc
     6001 aggttattca ccgcagcatt cagcgtcagg gcatccgtca tcttccacga cagacctgca
     6061 tccaccaccg tccaggcttt caggtattct cctttctcat catacacttt cttctgtaca
     6121 gcgctcagtg acgaataatt ctgggtgaaa cgtggtgttt tcccgcgata acgggcaccc
     6181 agccatgatg ccacctcttc ggtgatctgc cagttcagtt tcgcattcac catgtgttca
     6241 ggggtataac tcagcggcgc acctttgtta tcaccatcac gttgttcact tcgggtccag
     6301 gtgtaattca gtgacagcgt gacatcctct gaccacagcg gcaatgtgcc ggcaaattcc
     6361 acaccgtgca accgggcctt tccgctgttt acatagctat tggtgttatc atttatggaa
     6421 taagagacaa tcttgttgga gaagtcagtc ataaaacctg tgacattggc attcagaccg
     6481 gcggggttat cgtaatacac cccagcctca taactgacgc tctcttccgg cttcaggtcg
     6541 gggttaccaa gtagatttgt ttttccctgc ccggacacac cactaatccc tttatgtagc
     6601 tgccccattc tgggtgcctt atatcccgtg gtcacaccgc ctttcagcgt ccaggcatct
     6661 gccacatccc agaccagata tgcacgcgga ctgaagtgtc ccccgaattg ctcatgatgt
     6721 tcatagcggc tgcccgcagt cagcgcaagt gcatccgtga gatgccactc atcctcagca
     6781 aataccgacc agcttttctg ccggaaagtt tcacctgtgc tggcaaggac aactccgtct
     6841 ttcatggacg agctctgaaa ctcgcccccc accgtaacca gatgagattc tcccagaggg
     6901 gtaagcagta atgaattcag gataaggttc gattccttaa gctcccgcgg ctgaccggca
     6961 agcccccatt tgtcgcgctt cagtacactg cgtacgagct cacgaccttt attttctgtc
     7021 tcgttccagt tcagatacga tttccatgtt ccgaaggtga aagtatgatc atagccagct
     7081 gaaattttgt ttcgctcata gcgcagggtc cggtcatatc cccccgtcag actccccagt
     7141 tgcccatccc ggttatcata acgctgccgg gtggtatcca tatcaaacca gagcacatcc
     7201 tgctccgacg ccttccagtc aagacgtgca ccaagattat aattctgtga ctccgtggga
     7261 taaggaatac gcgtggctgc tgtatcgctc agtgatgtga ccgatgagcc ctgacgctgt
     7321 tgtgtgctac cgcgtacctg caggctgaca gaatcatcca caaggggacc actgctccag
     7381 aaattaaact ggctgctgtt accccatttg ttgctttcct gcagattcag ccctgcattg
     7441 acggaagaga gccatttgtc tgcattcttt ctggtaatga tattcaccac accgcccatc
     7501 gcatcagagc catacagtgt ggacatcggc cccctgataa cctcaatacg ctcaatggcg
     7561 gccagagggg gcatgaaccc ggtattcatg gcagaaaaac cgttgggagt cacgtcactg
     7621 cttccgccct gacgaacacc atcaatcagt atcagcgtgt aactggctgg cattcctcgg
     7681 atgctgattt ccagcccacc ggttttaccc gtaccacttt caacatccac accctctact
     7741 gatctcagag cctccgccag atcgtggtac tggctggact gcaattcctc ctggctaatc
     7801 acagaaacac tggcggctgc gttagtcagc tttttctcat agccggatgc cgagacaatc
     7861 atcacatcct ctgcagcagc tatgctgctg gctgaaaatc cgagacaggg aatgactacg
     7921 gaagccagag tggttattcg catattacta atctccgatc atgttaaccg gcaagacaat
     7981 acacaaatac aaacaataat gagaataatt tatatttacc ttttacaaaa aatgaatatt
     8041 actgacaaaa ctaaatataa ataatgactg cctcggcatg ccagttaaca gccatccgca
     8101 atggcgtgtt cgattttgcg aataaaaaat ggttactgta gaagcggatt ttcaccacct
     8161 gtcaaccatg aatggtcagt ggagtgagac gactggtatg atttggtcga tgacttcttc
     8221 cggtgaatac ggacagaaac attgaaatat tgtaagagag aacgttcaga attctgtgtc
     8281 acgtgaaaat actacagcgt tatcacctta ttcagtcgac cttcaaaata aacgtctaac
     8341 cgtgacaatc
//
LOCUS       DAZGCQ010000178         8224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000178 DAZGCQ010000000
VERSION     DAZGCQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.178"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..893
                     /locus_tag="ACHK07_004793"
     CDS             210..893
                     /locus_tag="ACHK07_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8105011.1"
                     /translation="MNLKKIFFSAVTVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     IELIEKQQAQLKSQLETIQKQQTGIITSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            937..4153
                     /locus_tag="ACHK07_004794"
                     /pseudo
     CDS             937..4153
                     /locus_tag="ACHK07_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4580..6235
                     /gene="lldP"
                     /locus_tag="ACHK07_004795"
     CDS             4580..6235
                     /gene="lldP"
                     /locus_tag="ACHK07_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8105012.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6235..7011
                     /gene="lldR"
                     /locus_tag="ACHK07_004796"
     CDS             6235..7011
                     /gene="lldR"
                     /locus_tag="ACHK07_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO8105013.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7008..8198
                     /gene="lldD"
                     /locus_tag="ACHK07_004797"
     CDS             7008..8198
                     /gene="lldD"
                     /locus_tag="ACHK07_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO8105014.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2037 a         1997 c         2188 g         2002 t
ORIGIN      
        1 taatcatcct tagaatccac aaaaataata tattcataca aacacagaca tcttttgaaa
       61 cgaatgatac atttttatga tgaatgctta aaacaatatt taatcgacac ccatcactgt
      121 cattaatgta aagcaacata gatcaccaat aatacaatgg acaactctac cggcattgat
      181 cgctggtcgg aggcaataag gatttttaca tgaacttgaa gaagattttt ttcagtgctg
      241 tcactgtttc agttctctgc acattaacgg gatgtgacta cattgaagaa ggcaagccag
      301 aaagtagttt actgaaacaa caagaagaac ataacaataa aattgagcta attgaaaaac
      361 aacaagcaca gctaaagagt caactggaaa ctattcaaaa acaacaaact ggcataataa
      421 ccagtacaaa gactttaact cacgtaatta aatccgttaa agatcaacaa aacactttta
      481 tttttaccga gtttaatccg gcaaaaacga agtatttcat cctgaacaat ggttcggtgg
      541 ctttagcggg ccgagtgtta tccattgacg caacagagaa tggcagtgtt attcatattt
      601 cactggtcaa cttattaagt ataccaatct caaacattgg ttttaatgcg acatggggtg
      661 gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca acaattactt ttcaacacat
      721 caatgaaatc cacattgaaa ttattaccag gtcaatggca agacattaat ttgacactga
      781 agggtgtatc gccgaataac ctgggatatc tgaagttagc catcaatatg gagaatattc
      841 agtttgacaa ccttccctct gctgaaaatc ggcagaaaag aagcaaaaaa taaatgcagc
      901 ttctgcgctt atatcaagga atagagaaca tcattaatga acaaaatatt taaagttatc
      961 tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag
     1021 aaatccgggc gcagtaagct gttaatttct gccgtggttg cgggtgggtt gttgtcgtcg
     1081 tttgaggcac tggcaacagg taatgaacct ggtacacctg ttggcacagg atctggctgg
     1141 gttgctatag gccaggaatc acaggcaaat gcttttaccg atggtaatgg tgccagtact
     1201 gcagtgggtt ataaagcttt cgctgaagga acatggagtt ccgctattgg cgccatgacc
     1261 catgcaattg gtccggcttc aatggcattc ggggttaatg ccgtctcaac cggtcaacga
     1321 agtatcgcta tgggcgcatc gtcaagttca gtggtcaata ttcgatggca cttgggcgat
     1381 atgcgaattc gggtggtgat tattcgatcg cgcaagggga taaatccaat gctgctggtc
     1441 tgaatgccat tgcagtggga agaagcagta gtgcggcagg cgagaaagcc actgcgctgg
     1501 gcaacgcagc taaagctact gagattatga gtattgctct tggcgacagc gccaatgcgt
     1561 caaaagcgta ttcaatggcg ctggtagcaa gtagcgtcgc atctgaagaa aacgcaattg
     1621 ccctggggcg tagcagttag ctagcggtac tgacagcctc gcattaggca gacaaacact
     1681 tgccagtaca gcgaacgcta ttgcgatagg tgctgagagc aaagccgctg aaaatgcgac
     1741 ggccgttggt accaaagcgg aggccaatgg acttaatagt atcgcgttgg gctctggggg
     1801 tattgcagat gccgataaca cgattgctct gggcaatcaa agcctggcag tagctgctgg
     1861 tgcaattgcc atcggtcagg ggaataaagc tgacggcgca aatgctatcg cgctgggtaa
     1921 tggtagtgtt acaggtggcg caaatgctat tgctcttgga caaggtagtt atgccggttt
     1981 agaaaatggc attgcaattg gtggtcaagc cagtgctcaa gggacaaatt ccgttgctct
     2041 gggtgcgggt tctgtagcga ctgaagataa ttccgtctct gtgggtaaca caacagatca
     2101 gcgaaaaatt gtcaatatgg cagcaggtga tatcagcact agcagtactg atgccataaa
     2161 tggatcacag ctttatgcta tcagtaagtc agtagcggac cgtcttggtg ggggggctac
     2221 cgtcaatgcg caaggtgtcg ttactgcccc gaattacaga ctgaaaaatg gtatttatgg
     2281 caatgttggc gatgctttag cggatctaaa caataacacg atacaatggg actccgttaa
     2341 aaagggatat agtgcggcac atggtacaga tgccaccagc accatcacca acgttaaagc
     2401 cggcactatt tctgctacca gtacggatgc ggttaacggt tctcagttga atgcgacgaa
     2461 tatgatgatt gagcagaaca gccaaatcat caatcagctt gctggtaaca ccgacgcaac
     2521 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acggtttagc
     2581 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     2641 gtctggtgcc agcagcgtag ccattggtca gaacagcaat agcaccgttg ataccggtat
     2701 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     2761 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     2821 attgtcaatt ggtaatgacg gtaaataccg ccaaatcatc aacgtagctg atggttccga
     2881 agcccatgac gccgttacgg ttcgtcaatt gcagaatgcg attggtgcgg tcgcaaccac
     2941 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     3001 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     3061 gaacactctg gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     3121 aaaccatgca aacagtattg caatgggtaa cggttctcag accacccgtg gtgcacagac
     3181 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgttgg
     3241 tagtgaagac ggtcaacgtc agatcaccaa cgtcgcagct ggttcagcgg ataccgatgc
     3301 ggtcaacgta ggtcagttga aagtaacgga tgcgcaggtt tcccagaata cccagagcat
     3361 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     3421 tattggcgat atcgtcacca ccggtagcat caagtacttc aagaccaata ccgatggtgt
     3481 agatgccagc gcgcagggta aagatagcgt cgcgattggt tctggttcca ttgctgccgc
     3541 ggataacagc gtcgcactgg gtacagggtc tgtggcaacc gaagaaaata cgatctctgt
     3601 aggctcatct actaatcaac gtcgtatcac caacgtagcc gcaggtaaaa acgacaccga
     3661 tgctgttaac gtggcacagt tgaagtcttc tgaggcgggc ggtgtacgtt acgacaccaa
     3721 cgctgatggt tctgttgact atagcaatat caccctcggt gggggcaacg gcggtacgac
     3781 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     3841 gaagcaaagc gtgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     3901 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     3961 cggtctgccg caggcttaca ctccaggtgc cagcatggcc tctattggtg gcggtactta
     4021 caacggtgaa tcggctgttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt
     4081 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     4141 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     4201 tgcgctacgc ttatcaggcc tacagggtga ttctgcacat tcctataggc cgggtaaggt
     4261 gttcacgccg catctggcaa gataaggcgc tccgggtcaa caatctaagg gcaattctct
     4321 gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg ccctaccaat
     4381 tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat caatgttgcc
     4441 aatacataac atttagttaa ctattcattg tcattatccc tacacaacac aattggcagt
     4501 gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca cgatgtgcgt
     4561 ggactccagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg ccgggaatat
     4621 ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg cgctgattaa
     4681 gctcaaactg aaaggatacg tcgccgcctc atggacggtg gcaatcgccc ttgccgtggc
     4741 tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt atggcttctt
     4801 ctacgggttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct ataagatctc
     4861 ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa ccccagacca
     4921 gcgcctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag gagccgcagg
     4981 ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggtctgggct ttaaaccact
     5041 gtacgccgcc gggctgtgcc tgattgttaa caccgcgcca gtggcatttg gcgcgatggg
     5101 cattccaatc ctggttgccg gacaggtaac aggtatcgac agctttgaga ttggtcagat
     5161 ggtggggcgg cagctaccgt ttatgaccat tatcgtgctg ttctggatca tggcgattat
     5221 ggacggctgg cgcggtatca aagagacgtg gcctgcggtc gtggttgcgg gcggctcgtt
     5281 tgccatcgct cagtacctta gctctaactt cattgggccg gagctgccgg acattatctc
     5341 ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcagc cagtgcgcgt
     5401 attccgtttt ggtgatttgg gggcgtcaca ggttgatatg acgctggccc acaccggtta
     5461 cactgcgggt caggtgttac gtgcctggac accgttcctg ttcctgaccg ctaccgtaac
     5521 actgtggagt atcccgccgt ttaaagccct gttcgcatcg ggtggcgcgc tgtatgagtg
     5581 ggtgatcaat attccggtgc cgtacctcga taaactggtt gcccgtatgc cgccagtggt
     5641 cagcgaggct acagcctatg ccgccgtgtt taagttcgac tggttctctg ccaccggtac
     5701 cgccattctg tttgctgcgc tgctctcggt tgtctggctg aagatgaaac cgtctgacgc
     5761 tatcagcacc ttcggcagca cgctgaaaga actggctctg cccatctact ccatcggtat
     5821 ggtgctggca ttcgccttta tttcgaacta ttccggactg tcatcaacac tggcgctggc
     5881 actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct ggctgggggt
     5941 attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc aagccaccgc
     6001 agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca ccggtggcgt
     6061 caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt
     6121 gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct tcacctgtat
     6181 ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc cttaatgatt
     6241 gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa
     6301 aaaaacctgg aagcgggcat gaagttgccc gctgaacgtc aactggcgat gcaactcggc
     6361 gtatcacgta attcactgcg cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc
     6421 agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc ggagcaaaac
     6481 atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt cgatattctg
     6541 gaagcacgct acgccattga agccagcacc gcatggcatg cggcaatgcg cgccacacct
     6601 ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga cccggatatc
     6661 gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca taacatcgtg
     6721 ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt
     6781 cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca tcaggctgtc
     6841 attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt
     6901 agttttgttc acaccacaat gaaacgattc gatgaagatc aggctcgcca cgcacggatt
     6961 acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg attatttccg
     7021 cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg ttccactata
     7081 tggatggggg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag
     7141 tggcgctgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa acgacgctgt
     7201 ttaatgagaa attgtcgatg ccggtggcgc taggtccggt aggtttgtgt ggcatgtatg
     7261 cgcgacgcgg cgaagttcag gcagccaaag cggcggatgc gcatggcatt ccgtttactc
     7321 tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt
     7381 ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa
     7441 aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg ggagcgcgtt
     7501 atcgtgatgc gcattctgga atgagcggcc cgaacgcggc aatgcgccgc tatttgcagg
     7561 cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtccg catgatttag
     7621 gaaatatttc ggcttacctc ggcaaaccga ccggactgga agattacatc ggctggctgg
     7681 ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc gatttctggg
     7741 atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt
     7801 ttggtgctga tgggattgta gtttctaacc acggtggccg ccagctggac ggtgtactct
     7861 cttccgctcg tgcgttgccc gctattgcag atgcggtgaa aggtgatata gccattctgg
     7921 cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca
     7981 ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag gcgggtgtag
     8041 ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg actggcgcga
     8101 aatcgatcag cgaaattacg caagattcgc tggtgcaggg gctgggtaaa gagttgcctg
     8161 cggcactggc tcccatggcg aaagggaatg cggcatagtc gtttgcgccc cctcccccta
     8221 accc
//
LOCUS       DAZGCQ010000179         8208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000179 DAZGCQ010000000
VERSION     DAZGCQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.179"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..350
                     /locus_tag="ACHK07_004798"
     CDS             <1..350
                     /locus_tag="ACHK07_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105015.1"
                     /translation="WIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSVDDGPV
                     MVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPETLFGSKSQDKHALFPIFT
                     ASWRANRIKNKGI"
     gene            573..1070
                     /gene="ubiC"
                     /locus_tag="ACHK07_004799"
     CDS             573..1070
                     /gene="ubiC"
                     /locus_tag="ACHK07_004799"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO8105016.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1083..1955
                     /gene="ubiA"
                     /locus_tag="ACHK07_004800"
     CDS             1083..1955
                     /gene="ubiA"
                     /locus_tag="ACHK07_004800"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO8105017.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2110..4533)
                     /gene="plsB"
                     /locus_tag="ACHK07_004801"
     CDS             complement(2110..4533)
                     /gene="plsB"
                     /locus_tag="ACHK07_004801"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO8105018.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPTHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4704..5072
                     /gene="dgkA"
                     /locus_tag="ACHK07_004802"
     CDS             4704..5072
                     /gene="dgkA"
                     /locus_tag="ACHK07_004802"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO8105019.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5182..5790
                     /gene="lexA"
                     /locus_tag="ACHK07_004803"
     CDS             5182..5790
                     /gene="lexA"
                     /locus_tag="ACHK07_004803"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO8105020.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5863..7188
                     /gene="dinF"
                     /locus_tag="ACHK07_004804"
     CDS             5863..7188
                     /gene="dinF"
                     /locus_tag="ACHK07_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO8105021.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLFGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFSAT"
     gene            7304..7513
                     /gene="yjbJ"
                     /locus_tag="ACHK07_004805"
     CDS             7304..7513
                     /gene="yjbJ"
                     /locus_tag="ACHK07_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO8105022.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7555..8070)
                     /gene="zur"
                     /locus_tag="ACHK07_004806"
     CDS             complement(7555..8070)
                     /gene="zur"
                     /locus_tag="ACHK07_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO8105023.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1856 a         2005 c         2241 g         2106 t
ORIGIN      
        1 agtggattcg tagtgatgat gtttcacctt tatttataga aattgaagat aatttaagaa
       61 cctttaacac gaatgaatta acacgaaacg ataatatctt tatcttgttc tcctctgtcg
      121 atgatgggcc agttatggtg gtaagctccc agcgcttaca tgatatgttg aatcctacaa
      181 aagataccaa ttggaattcc acgtatatct acaaatccag acatgagata ttgcctgtta
      241 atcttactcc ggaaacactt ttcggctcca aatctcagga taaacatgca cttttcccca
      301 tttttactgc gagttggcga gctaatcgta taaagaataa aggtatttaa gtaaaggaaa
      361 acatcaccgt tcctggcatc ctgggcggtg atgcccctat ggttgccctc gccagcacgg
      421 gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt catgcgattg ttgcgttttt
      481 gttgcgcatt agatcactta atttgcttta catctcccgt aaacactttt ctgcgataca
      541 atgcctttac gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta acgcaactgc
      601 gtgcgctgcg ctattttaaa gagatccctg ccctggagcc gcaactgctc gactggctgt
      661 tgctggaaga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg agcgtgacga
      721 tgatccgcga agggtttgtc gagcagaatg aaattcccga agaactgccg ctgctgccga
      781 aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa ccgtggcttg
      841 ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc ggagctggcg ttacaaaaat
      901 tgggtaaaac gccgttagga cgctatctgt tcacatcatc gacattaacc cgggacttta
      961 ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga ttaagcggta
     1021 aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa gaggaaaaaa
     1081 atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta atgcgtacgg
     1141 ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg tgggtggcga
     1201 caccgggcgt tccccagctc tggatcctgg cggtgtttgt cgcgggtgtc tggctgatgc
     1261 gcgctgccgg atgtgtggtg aatgattatg ctgaccgcaa gtttgatggt catgttaagc
     1321 gcacggcgaa ccgaccactt cccagcggcg cggtaacaga gaaagaggcg cgcgcgctgt
     1381 ttgtagtgct ggtactgatt tcgtttttac tggtgctgac gctgaatacg atgaccattc
     1441 tgttgtcgat tgccgcgcta gcgctggcgt gggtgtaccc gtttatgaag cggtataccc
     1501 atctaccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg gcttttgctg
     1561 ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat attctctggg
     1621 cggtggctta cgacacgcag tatgcgatgg ttgaccgtga tgatgatgtg aagattggca
     1681 ttaaatccac ggcaatcctg ttcggccaat acgataaatt gattattggt attttgcaga
     1741 ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa tggcttaggc tggggatatt
     1801 actggtcaat tctggtggct ggcgcgctgt ttgtttatca acaaaaactg attgccaacc
     1861 gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt ctggtactgt
     1921 ttttagggct ggcaatgagt tactggcatt tctgatgatg taaaaaagcc ggatgatcat
     1981 ccggctttct tctgggttgc ctgatgcgct gcgcttctca ggcctacaca acagatcgca
     2041 atttattgaa tttgcagatt atggaaggcc ggataaggcg tttgcgccgc atccggcaat
     2101 tctctctgat tacccttcgc cctgcgttgc actctcaatc gtcaaacgca cgtctgatgt
     2161 aatcaactcc gccagcaact gataaacctt catcgtttct gccggttcgg catcgccgct
     2221 atcgctgata tacccttcat cacgtagcgt cagcaccaga gaactgaaca ccgccttgtc
     2281 gaagaactcc ggcgcgttga taccgtgcag cacggagaga cgttgcgcga cggtgcggct
     2341 ctctttctcc agcgtaccgc ggttgatcga cgggttggcg ctcaacagcc agaaggtgat
     2401 agcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtagcg tgcgagaatg
     2461 cgtcgggttg atatgcaact catcatcttg cagggtaatc agcccctgac gttgcatctc
     2521 atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga tcccagcgca ggaacagctc
     2581 cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc gggagatgtg
     2641 gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat gcgcaatgtt
     2701 gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc agaatgatga tgtcgccgat
     2761 agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg tgatcgataa gctcgctggc
     2821 gctggctgaa ggaacggtag agtccgtgga gtaaggcacg ttgcgcatca gatccaggta
     2881 gcagttgagt tgctcggtta actgctcgcg ggtgagtgag cgctgacgtg atgccagtag
     2941 cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg ttgttaatgc gtaccatcag
     3001 atcggcagca atattattga ccgtcggtgt taaccatgcc ggacgcaccg cttcgatggg
     3061 atcgatagat tcacgccagt caggtacgtg ctggttaagg taggtcatca acggcattgg
     3121 ttcaccgaaa ttgacgtaac cctgaccgag attacgcagc ttgcttaaac cgcgcagcat
     3181 ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt tctttggcgt aagtacccac
     3241 ttccatgacg tgctcataac cgatatagat cggaatcagc gtaatcggac gcgtgccgcc
     3301 acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt ttcggatcca gcaaacgccc
     3361 cgtacgggaa cgaccgcctt ctacgaagta ctcgacggaa taaccacggc tgaacagttc
     3421 gccgagatac tcacggaaaa cggtggaata gagcttattg cctttaaaag tacggcgaat
     3481 aaagaacgca cccagacggc ggaaaatcgg cccggccggc cagaagttca ggttgatccc
     3541 ggcggcgata tgcggcggca ccagcccctg gtgatacagc acgtaagaaa gcagcaggta
     3601 gtccatgtga ctgcggtggc aaggcacata caccagctca tgaccgtcgt gggccaactg
     3661 gcgaacgcgc tcggcgttat ggacgttgat accctggtaa agtcggttcc aggtgaagcc
     3721 cagaatacgg tcagtcaggc gaatcatctc gtaagagaaa ttcgccgcaa tctcttccat
     3781 cagtgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc gcgcttcatc
     3841 ttctaccgct ttcgcaatgg cgcgggaggc gagcagctta ttaaacagat cctgacgagc
     3901 aggcaaacgt gggcctacgg cagccagacg ttgacgggca aagtgcatac gcgccacgcg
     3961 cgccagtttc tgcgcgatag ttttatccgt gccgtgttca tccgccatac ggcgcagcga
     4021 aacggacggt gagaaacgca caaaactgtc gcgaccgagc cacagtacag cgaaaaattt
     4081 ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg cctttttcac gccccggcgc
     4141 gcgaccaaac atcaccgaca ctggcaccat ctgcacatcc agatttgggt tgctacggtg
     4201 caaatcgaga tagtcgtgga acagcttaat agactcttct ttcggcgtgt aataggtgaa
     4261 tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt cgatttccag
     4321 cggctctaac gggtcaggca agtcatgtgc cagacactgg gcgcgcaacg tcagcaagtc
     4381 tgctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca gccccagttc
     4441 cggggcagga tctgccggaa tagacttgct ttttaccagg atgcttaatg gtaaattcag
     4501 taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc tggttaataa
     4561 tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat ccgctcatgt
     4621 ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga tattgacgct
     4681 cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcacc cgaataatca
     4741 aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa gcggcattcc
     4801 gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc ctgctggctg gatgtggacg
     4861 cgattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg gaaatcctca
     4921 atagcgccat cgaagcagtg gttgaccgaa ttggctctga ataccatgag ctttccggac
     4981 gcgctaaaga tatgggatcc gccgcggtgc tgattgcgat tatcgtcgcc gtgattacct
     5041 ggtgcatcct gttatggtcg cattttggat aacccttcca gaattcgata aatctctggt
     5101 ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt atatactcac agcataactg
     5161 tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat
     5221 ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag
     5281 cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa
     5341 ggcgtaattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca ggaagaggaa
     5401 gaagggttgc cactggtagg tcgtgtggct gccggtgaac cgcttctggc gcagcagcat
     5461 attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga tttcctgctg
     5521 cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgactt gctggcagtg
     5581 cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga tgacgaagtt
     5641 accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga aaatagcgag
     5701 tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaagg gctggcggtt
     5761 ggcgttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat gccgcctggc
     5821 gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt cctcacttca
     5881 tctgataaag cactttggca tctcgcctta cccatgattt tctccaatat caccgttccg
     5941 ttgctgggac tggtcgatac ggcggttatt ggtcatcttg atagcccggt ttatttgggc
     6001 ggcgtggcgg ttggtgcaac ggcaaccagc tttctcttta tgctgttgct gtttttacgc
     6061 atgagcacca ccgggctgac tgcgcaggct tatggtgcca aaaatcctca ggcattagcc
     6121 cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggctg gggcgttaat tgcgctgctg
     6181 cgtacaccga ttatcgatct ggcgctgcat attgttggcg gtagtgaagc agtcctggaa
     6241 caggcgcggc gctttcttga aatccgctgg ttaagcgcac cggcgtcgtt ggcgaatctg
     6301 gtattactcg gttggttact cggcgtgcaa tatgcccgtg cgccagtaat tttgttagtg
     6361 gtcggcaata tcctcaacat tgtgctggat gtctggctgg taatggggct gcatatgaac
     6421 gtgcagggtg cggcgctggc gacggttatt gcggaatatg caacattgct gtttggtctg
     6481 ctaatggtgc gtaaaatcct caaactacgc ggaatttccg gcgaaatgct gaaaactgcc
     6541 tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg atatcatgct gcgttcgctg
     6601 ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg cgcgactggg gagtgacatt
     6661 atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc gctggatggt
     6721 tttgcctacg cggttgaagc acattccggt caggcatacg gtgcgcgcga cggtagccag
     6781 ttgctggatg tctggcgggc agcgtgccgc cagtcgggga tcgtagcgtt actgttttcg
     6841 gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt aacccagatt
     6901 cagcagctgg ccgaccgcta tcttatctgg caggtgattt tgccgttggt tggcgtctgg
     6961 tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat gcgtaacagt
     7021 atggcggtgg ccgccgcagg ttttgcgctg acgctcctta cgctgccgtg gctgggcaat
     7081 catggtttgt ggctggcgtt aaccgtcttt ctggcgttgc gcgggctttc tctggcggct
     7141 atctggcggc gtcactggcg caatggtacc tggttttccg caacgtgacg gttaaaaatt
     7201 ctgaataaat aatcctaagc caaattgctg actacactta atctcacgtt cagaagaaaa
     7261 gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata aagatgaagc
     7321 cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca aactgaccga
     7381 tgatgatatg acgatcattg aaggtaaacg tgatcaactg gtcggtaaaa tccaggaacg
     7441 ttatggttat cagaaagatc aggcagaaaa agaggtcgtg gattgggaaa cccgcaatga
     7501 atatcgctgg taattaatcc ctcctgcccg acgtgtacaa ggatgtacgc cctcttaacg
     7561 cggtttcttt ttcacctgca cagagtgatc atgctggcac tgttcaggat gacgacacgc
     7621 ttccacttcc acgcatgccg cacagagccc atgtgcttca atcacattat gccgcagggc
     7681 aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc acgccttctg cacattcttc
     7741 tttcaccgcg ccgcatcgat cgcaaataaa catagctgac gtatgggtgg gctggtcgaa
     7801 cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac cttgctcaag
     7861 cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag cttcgcgcag
     7921 caaatcaagc agatcgtaag cgctgatagc gccatcctgc agactcatca ggcgcaacac
     7981 ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg cagatttttt cagcctgcgc
     8041 taataactcc tgcgttgtgg tcttttccat cgggcacctc aaagtggggg ttaagaatgt
     8101 ccttacttta ccatgttcca ggaaaaacac cgagaaccct tattgttgtc gtaatgttga
     8161 ttttctgttt tgtacgtaag gtgttgtttt gcctggtcgt accattat
//
LOCUS       DAZGCQ010000180         8151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000180 DAZGCQ010000000
VERSION     DAZGCQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.180"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..968
                     /gene="rluB"
                     /locus_tag="ACHK07_004807"
     CDS             93..968
                     /gene="rluB"
                     /locus_tag="ACHK07_004807"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO8105024.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1008..1598)
                     /gene="cobO"
                     /locus_tag="ACHK07_004808"
     CDS             complement(1008..1598)
                     /gene="cobO"
                     /locus_tag="ACHK07_004808"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO8105025.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1595..2353)
                     /locus_tag="ACHK07_004809"
     CDS             complement(1595..2353)
                     /locus_tag="ACHK07_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO8105026.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2573..3622
                     /gene="sohB"
                     /locus_tag="ACHK07_004810"
     CDS             2573..3622
                     /gene="sohB"
                     /locus_tag="ACHK07_004810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO8105027.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3658..3909)
                     /locus_tag="ACHK07_004811"
     CDS             complement(3658..3909)
                     /locus_tag="ACHK07_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO8105028.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4289..6886
                     /gene="topA"
                     /locus_tag="ACHK07_004812"
     CDS             4289..6886
                     /gene="topA"
                     /locus_tag="ACHK07_004812"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO8105029.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKHQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            7096..8070
                     /gene="cysB"
                     /locus_tag="ACHK07_004813"
     CDS             7096..8070
                     /gene="cysB"
                     /locus_tag="ACHK07_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO8105030.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         2070 a         1934 c         2168 g         1979 t
ORIGIN      
        1 ctttactatc agtgagtcag atgtaagttc tgactcactg ttgcacaaaa gcatatcaga
       61 cgcctgggaa ggcgacacca aaaggaagct ctatgagcga aaagctacag aaagtgctgg
      121 cgcgtgccgg ccacggttct cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
      181 gtgttgatgg caaaattgct aaactcggcg atcgcgttga agttacccct ggattgaaaa
      241 tccgtatcga tggtcacctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
      301 tggcctatta caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa ggacgtccaa
      361 cggtgtttga ccgtctgcca aaactgcgtg gcgcacgctg gattgccgtg ggtcgcttgg
      421 acgttaatac ctgtggtctg ctgctgttca ccaccgatgg tgaactggca aaccgtttaa
      481 tgcacccaag ccgtgaagtt gaacgtgaat atgccgtgcg tgtatttggt caggttgacg
      541 acgcgaaact gcgtgatttg agtcgtggcg tgcagttgga agatggtccg gcagctttta
      601 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg
      661 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc
      721 tgatccgtgt tcgttacggt gatatcccac tgccaaaagg tctgcctcgc ggtggctgga
      781 cggagctgga cctcgcccag actaactatc tgcgcgaact ggtggagcta ccgccagaaa
      841 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat gaaggcgaat cagattcgtc
      901 gtgcggtgaa acgtcacagt caggtgagcg gcggtcgtcg ttctggcgga cgtaataata
      961 acggttaatc aaagtatgcc ggacgtcata tccggcattt ttacagatta ataatcgatc
     1021 cctatctgcg cttttacacc ggcatcaaac gcatgtttga tggggcgcaa ctcactcacc
     1081 gtatccgcca gttcaagaat atcccgatga cagcctcgac ccgtgataat caccgtctgt
     1141 tgatgtggac gttcatttaa cgcctgcaac acttcttcca gtggcaaata gtcatacgcc
     1201 accatatatg tcagctcatc gagcaaaacc atatccagtg aggaatcagc aagcatccgc
     1261 tttgcatgtt gccagacttc gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc
     1321 caggtaaagc ccgttgccat cacctgaaac tcaacgccat gtggctccag cagattgcgt
     1381 tcgccattag gccaggtgcc tttaataaac tgcacgacgc ccactttttt tccgtgacca
     1441 actgcgcgtg ttgccgtacc aaatgccgcg gtggttttgc cttttccatt gccggtaaag
     1501 acgatgataa taccgcgttc atcctgggcc tgggccacac gagcatctac tttttctttc
     1561 actcgctgct gacgctgttg gtagcgttca tcactcattg ggaaattcct ggtttacggc
     1621 ccggttgggc gtcaaaggtc atgccggttt tacggcggct gtcatcgccc atcagccaga
     1681 ggtagagcgg catgatatca gcgggagttt taagtttctg tggatcttcg gtcgggaagg
     1741 cgctggcacg cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg cgcaggcgct
     1801 gctgatattc atcggccagt acctgcatca tcccttcggt ggcaaatttc gacgctgcat
     1861 atgcgcccca gttggctcgt ccctgacgtc caacgcttga tgaagtaaag accagtgaac
     1921 cggcgtccga tttgagtaat aaaggaagca gtgcctgggt gagcataaag gtggcattaa
     1981 cgttgacctg tatgacgtcc tgccagacct gtggattttg ttcgctcatt ggacaaacat
     2041 cgccgagcaa tccggcatta tgcaaaacac catccagacg cggataatta acggcaatgc
     2101 gctgtgccag ttgttggcaa tcttcggacc tgcaggtcag caaatcgaga ataaaccact
     2161 gtggctgacg cccagtttct tcgtttatgt ggctggctac ctgacgtaat ttttcttcat
     2221 tacggcccaa cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg gcttcacgac
     2281 caataccatc actggctccc gtcaccagga taatgcgatc attgagtaaa tcttgttttg
     2341 gctggtaatg catggctact cctcaacgac gttgtctgtc gcgctgccag tgtactttat
     2401 gactttgggg ctttatgcct gaaacagaag ggcatttcaa tcagcctggg gcgataacac
     2461 cgcaaaatgg atactttgtc atactttcgc tgcaataaca tctctgcgag acggcttaac
     2521 atgcctgttg taaactgtga gccaaagcgt tgtttaacca aggtggggac tcgtggaatt
     2581 gttgtctgaa tatggtttgt ttttggcgaa aatcgttacc gttgtgctag cgattgcggc
     2641 gattgccgcc attattgtca atgttgctca acgtaataaa cgccagcgtg gcgagttacg
     2701 ggtcaacaat ctcagcgaac agtataagga gatgaaagaa gaactggccg cggcgctgat
     2761 ggactcacat cagcaaaaac agtggcacaa agcgcagaag aaaaagcata agcaagaagc
     2821 gaaagcagca aaagcgaaag ccaaactggg cgaggtggca actgacagta aaccccgcgt
     2881 ctgggtgctg gattttaaag gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga
     2941 gataacggct gtactcgcag cattcaaacc gcaggatcag gttgtgctac gtctggaaag
     3001 ccctggtggc atggtgcatg gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga
     3061 taaaaacatt cctttaactg ttacggtaga caaagtcgct gccagcggcg gttacatgat
     3121 ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct attgtgggtt ccattggtgt
     3181 ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc aaagatattg atatcgaact
     3241 gcacaccgcc gggcagtata agcgcacgct gaccttgctg ggtgaaaata ccgaagaagg
     3301 gcgggagaaa ttccgcgaag agctgaacga aacgcatcag ttatttaaag attttgtgaa
     3361 gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg ggtgaacact ggtacggaca
     3421 acaggcggta gagaaaggcc tggttgatga aatcaacacc agtgatgaag ttattcttag
     3481 cctgatggaa ggccgtgaag tggtcaatgt acgctatatg cagcgtaaac gactcattga
     3541 ccgattcacc ggcagcgcgg cagagagcgc cgatagatta ttgctacgct ggtggcagcg
     3601 tgggcaaaag ccattgatgt aaaagacaaa cgcgagggta agacctcgcg ttttgcttta
     3661 atcaaccaga tgatattttt ctgaaagcac atgggccagg tgtttgaaca tattaaacac
     3721 cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac
     3781 cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg
     3841 ctcacgaatg attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc
     3901 tttgttcatt atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga
     3961 caaatatacc acgctggtgg caagagcgcc ttactggcaa ctttggattt tgcatgctaa
     4021 taaagttgcg tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag
     4081 atttgcttga cattcgacca aaattccgtc gtgctatagc gcctgtaggc caagacctgt
     4141 taactcagtc acctgaattt tcgtgaacag agtcacgaca aggggttgat atccgcagag
     4201 agcgagtcca tatcggtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc
     4261 tggaagaatc aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc
     4321 ggcaaaagcc aaaacgatca acaagtatct gggtagtgac tacgtggtta aatccagcgt
     4381 cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac
     4441 ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat
     4501 gggggttgac ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa
     4561 ggtcgtctct gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga
     4621 ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg gaagtgattg gtggtgatga
     4681 tgcgcgctat agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcgtt
     4741 taacaaaccg ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat
     4801 ggaccgcgtg gtggggtata tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct
     4861 gtctgccggt cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg agcgtgaaat
     4921 taaagcgttc gtgccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg
     4981 tgaagcgttg gcgttgcagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa
     5041 agaacaaact caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg
     5101 tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca
     5161 agctgccagc acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt
     5221 gtatgaagca ggctatatca cttacatgcg taccgactcc actaacctga gtcaggacgc
     5281 ggtaaatatg gttcgcggtt atatcagcga taattttggt aagaaatatc tgccggaaag
     5341 tccgaatcag tacgccagca aagaaaactc acaggaagcg cacgaagcga ttcgtccttc
     5401 tgacgtcaat gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc agaaactgta
     5461 ccagttaatc tggcgtcagt tcgttgcctg ccagatgacc ccagcgaaat atgactccac
     5521 gacgctgacc gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta ttttgcgctt
     5581 tgatggctgg acaaaagtga tgcctgcact gcgtaaaggc gatgaagatc gtatcttacc
     5641 ggcagtcgat aaaggcgatg ctctgacgct cgttgaactg acaccagccc agcactttac
     5701 caagccgcca gcccgtttca gtgaagcatc gctggttaaa gaactggaaa aacgcggtat
     5761 cggtcgtccg tctacctatg cgtcgatcat ttcgaccatt caggatcgtg gctatgtgcg
     5821 tgtagaaaac cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg atcgcctgga
     5881 agagaatttc cgcgagttaa tgaactacga cttcaccgcg cagatggaaa acagcctcga
     5941 ccaggtggca aatcacgaag cagagtggaa agctgtactg gatcacttct tctcggattt
     6001 cactcagcag ttagataaag ctgaaaaaga tccggaagag gggggtatgc gtccgaacca
     6061 gatggttctg accagcatcg actgcccgac ctgtggtcgc aaaatgggga ttcgcacagc
     6121 gagcaccggg gtattccttg gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa
     6181 aacaaccatt aacctggtgc cggaaaacga agtgctgaac gtgctggaag gcgaagacgc
     6241 tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag
     6301 ctatctcatc gatccgaaac gtaagttgca tgtctgtggt aataacccaa cctgcgacgg
     6361 ttacgagatc gaagagggcg aattccgcat taaaggttat gacggcccga tcgttgagtg
     6421 tgaaaaatgt ggttctgaaa tgcacctgaa aatggggcgt ttcggtaaat atatggcctg
     6481 caccaacgaa gagtgtaaaa acacgcgtaa gattttacgt aacggcgaag tggctccacc
     6541 gaaagaagat ccggtaccat taccggagct gccgtgcgaa aaatcagatg cctatttcgt
     6601 gctgcgtgac ggtgctgccg gtgtgttcct ggcggccaat accttcccga aatcgcgtga
     6661 aacgcgtgcg ccgctggtgg aagagctgta tcgcttccgc gatcgtctgc cggaaaaact
     6721 gcgttatctg gccgatgcgc cgcagcagga tccggaaggt aataagacta tggttcgctt
     6781 tagccgtaaa accaaacatc aatatgtctc ttcggaaaaa gacggaaagg cgactggctg
     6841 gtcagcattt tatgttgatg gcaaatgggt tgaagggaaa aaataacctt taattctgtc
     6901 aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat tattttttct
     6961 tcacacctat acactaaggc tataaatgat atagtggtta tagttagcac cttttttatt
     7021 attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta
     7081 agtggatggt ttaacatgaa attacaacaa cttcgctata ttgttgaggt ggtcaatcat
     7141 aacctgaatg tctcatcaac agcggaagga ctttacacat cacaacccgg gatcagtaaa
     7201 caagtcagaa tgctggaaga cgagctaggc attcaaattt tttcccgaag cggcaagcac
     7261 ctgacgcagg taacgccagc aggacaagaa ataattcgta tcgctcgcga agtcctgtcg
     7321 aaagttgatg ccataaaatc ggttgccggt gagcacacct ggccggataa aggctcgctg
     7381 tatatcgcca ccacgcatac ccaggcacgc tacgcgttac caaacgtcat caaaggcttt
     7441 attgagcgtt atccccgcgt ttctttgcat atgcaccagg gctcgccgac acaaattgct
     7501 gatgccgtct ctaaaggtaa tgcagatttc gctatcgcca cagaagcgct gcatctgtat
     7561 gaagatttag tgatgttacc gtgctaccac tggaatcggg ctattgtagt cactccggat
     7621 cacccgctgg caggcaaaaa agccattacc attgaagaac tggcgcaata tccgttggtg
     7681 acatatacct tcggctttac cggacgttca gaactggata ctgcgtttaa ccgcgcaggg
     7741 ttaacgccgc gtatcgtctt cacggcaacg gatgctgacg tcattaaaac ttacgtccgg
     7801 ttagggctgg gggtaggggt cattgccagc atggcggtgg atccggtcgc cgatcccgac
     7861 cttgtgcgcg ttgatgctca cgatatcttc agccacagta caaccaaaat tggttttcgc
     7921 cgtagtactt ttttgcgcag ttatatgtat gatttcattc agcgttttgc accgcattta
     7981 acgcgtgatg tcgttgatgc ggctgtcgca ttgcgctcca atgaagaaat tgaggtcatg
     8041 tttaaagata taaaactgcc ggaaaaataa tttcttgcgt tattttcggc accttttttg
     8101 tagcgaaggt gccgaaatat attttctttt gttacttatt tgtatatcgt g
//
LOCUS       DAZGCQ010000181         8054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000181 DAZGCQ010000000
VERSION     DAZGCQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.181"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..806
                     /locus_tag="ACHK07_004814"
     CDS             <1..806
                     /locus_tag="ACHK07_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO8105031.1"
                     /translation="IDLFIQRITHNLWHARKKNVFLIEQITNLKEWVNKYIGDCTDEE
                     LNDRDFIASVVDRAIFHFAINSICNPEDNKDATPIERYTFDVETKNGLPSTVQLFYEE
                     SKDNEPLANIHLQAIGSGFLTFVNACQKYDDNSLKLFASLLISLSYSSAYTDLAGAEK
                     VNINDDNENYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGKLA
                     PDILSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            1169..2206
                     /gene="dusA"
                     /locus_tag="ACHK07_004815"
     CDS             1169..2206
                     /gene="dusA"
                     /locus_tag="ACHK07_004815"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO8105032.1"
                     /translation="MYGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2340..2582
                     /gene="pspG"
                     /locus_tag="ACHK07_004816"
     CDS             2340..2582
                     /gene="pspG"
                     /locus_tag="ACHK07_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO8105033.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2748..3731)
                     /locus_tag="ACHK07_004817"
     CDS             complement(2748..3731)
                     /locus_tag="ACHK07_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO8105034.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3814..5229
                     /gene="dnaB"
                     /locus_tag="ACHK07_004818"
     CDS             3814..5229
                     /gene="dnaB"
                     /locus_tag="ACHK07_004818"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO8105035.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5282..6361
                     /gene="alr"
                     /locus_tag="ACHK07_004819"
     CDS             5282..6361
                     /gene="alr"
                     /locus_tag="ACHK07_004819"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO8105036.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6614..7807
                     /gene="tyrB"
                     /locus_tag="ACHK07_004820"
     CDS             6614..7807
                     /gene="tyrB"
                     /locus_tag="ACHK07_004820"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO8105037.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNALPHGASLYLPMEGLNSYRHAIAPLLFGADHSVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1981 a         1890 c         2082 g         2101 t
ORIGIN      
        1 aaatagattt gtttattcaa agaatcactc ataatttatg gcatgcacgt aaaaaaaatg
       61 tgtttttaat tgagcaaata actaatctca aagagtgggt taataagtat attggagatt
      121 gcactgatga ggaattaaac gatcgtgact ttattgcatc agttgttgac cgggctattt
      181 ttcatttcgc gattaatagt atatgtaatc ctgaggataa taaagatgca acgcccatag
      241 aacgatatac ttttgatgta gaaaccaaga atggccttcc ctccacggtt cagttatttt
      301 atgaagaatc taaagataat gaacctttag caaatataca tttacaagca ataggttctg
      361 gttttttaac gtttgttaat gcatgtcaga aatatgatga caacagctta aaattattcg
      421 cttcgctgtt gatttcactt tcatactcta gtgcctacac agatttagca ggagcagaaa
      481 aagtgaatat taatgatgat aatgagaact atctgacagc tcagtttgaa gagttatctc
      541 aacgtgatat gaagaagtac ctgggagaga tgaagcgtct ggcagatggg ggagaaatga
      601 attttgatgg ctatctggat aagatgtcac atctggtgaa tgaaggaaag ctcgcccctg
      661 atattttaag caaaatgcga gatgctgcac cgaaattaat tgactttgct aagtcgtttg
      721 acccaaactc aaaggaaaag attaaaatat atacaggcac ttctaaatta atttatgatt
      781 tattcggggt taaatcggag aaataatatg tgaagttctt cgatggtatg gaaggcatta
      841 cataaaagaa cccaatgctt attgggttct ttttttctct atcagtgcta ttagcaggga
      901 gagatatcac cggagtttaa tgtgtgattt ttatttatcg tcgaacctgg attgtttatc
      961 attggcctta acaaagctaa cggctaataa gattatttcc atcacttcgt gagagcttca
     1021 tgccttgaga ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt
     1081 atagtatccc gctattgagc ctcctgaaca gtgatgctga ataacataac cccatgatat
     1141 atcgataaaa taatttctac atttgaaaat gtacggtaat tctgaaatgc aaaaaatcaa
     1201 ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc
     1261 accaatgctc gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa
     1321 tacgttgctg tataccgaaa tggtgactac aggggcgatt attcacggta aaggtgatta
     1381 cctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcggcggta gcgatccggc
     1441 ggcgctggcg cagtgtgcga agctggcaga agcgcgtgga tatgatgaga tcaacctgaa
     1501 tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa
     1561 tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccggtgac
     1621 ggtgaaaacg cgtattggca tcgatgacca ggacagctat gaatttctct gcgatttcat
     1681 caacaccgtt tccggcaaag gcgagtgtga gatgttcatc atccacgcac gtaaagcctg
     1741 gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt atccgcgtgt
     1801 gtatcaactg aagcgtgact ttccgcatct gacgatgtcg attaacggtg gtatcaagtc
     1861 gctggaagag gctaaagcgc atttgcaaca tatggatggc gtgatggtcg ggcgcgaggc
     1921 gtatcagaat ccgggtattc tggcggcggt agaccgagag atctttggtt cctcggatac
     1981 cgatgccgat ccggtggcgg tagtgcgcgc catgtatccg tacattgagc gtgaactcag
     2041 ccaggggacg tatctcggcc atattacccg gcatatgctg ggcttgttcc agggtattcc
     2101 tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgctgacat
     2161 taacgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa
     2221 gtagtcaaat tcaccacgcc ctgcgcaaat tcgcggggcg ttttgctgtt aaatcaatag
     2281 attatttttg gcatgattct tgtaatgcca gcaagagatt tcatatttgg gagagcatca
     2341 tgctggaact actttttgtg attggctttt ttgtcatgtt gatggtcacc ggcgtttcgt
     2401 tgctgggcat tatcgccgcg ctggttgtgg cgacggccat tatgttcctc ggcggtatgc
     2461 tggcattgat gattaagttg ctgccgtggt tactcctggc gattgcggtg gtgtgggtta
     2521 ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact
     2581 aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga
     2641 taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag
     2701 ccgaactata aaaagaaagg gcttcccagg tggaagccct atttctttta tggaatcaac
     2761 aggctggaac cttgcgtcgc ccggctttcc agaatctcat gcgcacgctg cgcatccttc
     2821 agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag
     2881 aacagttcat tactggcctc ggttaattcc tcccgcgtgg tgatatagcc ttgcagggaa
     2941 gggcgtgtca catacaacga gcctttctga ttgagaatgc ctaagttcac accggtaacc
     3001 gcacctgatg agttgccaaa actcaccatt aagccgcggc gttgcaggca atccagcgac
     3061 cgttcccagg tgtctctgcc cacggaatcg tacaccacgc gcactttttt accaccggtg
     3121 atctctttta accgctcgac cagattctct tcacgatagt taataacctg ccacgcgccc
     3181 gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgccc
     3241 agggcttttg cccactggca ggcaatcaag ccaacgccgc cagccgctgc gtggaacagg
     3301 aactgttcat cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct
     3361 ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta
     3421 tccgcaataa tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc atagactaca
     3481 cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgctgct
     3541 tcggtgccta atccgctggg tagcgatggc ggcgggtaaa ggccgctgcg gatatatgtg
     3601 tcgataaaat tgatgccgat ggctttattt tcgacctgga tttcattctc cgccggatcg
     3661 gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt
     3721 cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc
     3781 gacccattct ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa
     3841 cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac
     3901 tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga acgctgggat
     3961 gatgtagccg agcgtgtggt agcagacgat ttttacaccc gcccacaccg tcatatcttt
     4021 actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac ccttgcggaa
     4081 tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg
     4141 tcaaaaaata cgccaagtgc ggcgaacatc agtgcctatg cggacatcgt gcgtgaacgt
     4201 gccgttgtcc gtgagatgat ctcggttgcg aatgagattg ccgaagccgg ttttgatccg
     4261 caggggcgta ccagcgaaga tctgctggac ctggctgaat cccgcgtctt taaaattgcc
     4321 gaaagtcgtg cgaacaaaga cgaagggccg aagaacatcg ccgatgtgct cgacgccacc
     4381 gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg ggtaaacacc
     4441 ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc
     4501 gccgcgcgtc cgtcgatggg taaaacgaca tttgcgatga acctcgtcga aaacgcggcg
     4561 atgttgcagg ataaaccggt acttatcttc tcgctggaga tgccatcaga acaaattatg
     4621 atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac cgggcagctc
     4681 gatgatgaag attgggcacg catttccggc accatgggta ttttgctcga aaaacgcaat
     4741 atctatatcg atgactcctc cggcttgacg ccaacggaag tgcgttcccg cgcacgccgt
     4801 attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc
     4861 gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctcccg ctcgctgaaa
     4921 gcactggcga aagaactgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg
     4981 gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag
     5041 caggatgcgg acttgatcat gtttatctat cgtgatgagg tgtatcacga aaacagtgat
     5101 ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggta
     5161 cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac
     5221 gacgaataat aattatttta tgaattaggt aattaaagca aacacttatc aaggaacaca
     5281 aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct
     5341 tcgtgaactg gctcctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca
     5401 cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct
     5461 cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg
     5521 cttttttgat gccagagatc taccgacgat ttctgcgcaa cattttcata ccgccgtgca
     5581 taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg
     5641 gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt
     5701 ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt
     5761 tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa caactcgcta tctttaatac
     5821 cttttgcgaa ggcaaaccag gtcaacgttc cattgccgcg tcgggtggca ttctgctgtg
     5881 gccacagtcg cattttgact gggtgcgccc gggcatcatt ctttatggcg tctcgccgct
     5941 ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca gtgatgtcac taacctccag
     6001 cctgattgcc gtgcgtgagc ataaagccgg agagcctgtc ggttatggtg gaacctggat
     6061 aagcgaacgt gatactcgtc ttggtgtagt tgcgatgggc tatggcgatg gttatccgcg
     6121 cgccgcgccg tctggtacgc cagtgctggt gaacggtcgc gaagtgccga ttgtcgggcg
     6181 agtcgcgatg gatatgatct gcgtagactt aggtccacag gcacaggata aagccgggga
     6241 cccggtcatt ttgtggggtg aaggtttgcc cgtagaacgt atcgctgaaa tgacgaaagt
     6301 aagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta
     6361 atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccac
     6421 taaaatccta ccaattcaat aagttgatat tctttcatgc tcttataaag gtcgtgcctc
     6481 tggcggatgt acgtttgtca tgagtctcac tctgttgcta attgccggtc gcccctgaac
     6541 atccactcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct
     6601 ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct
     6661 tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagta tcggtctgta
     6721 ctacaacgaa gacggaatta ttccacaatt gaaagccgtg gctgatgcag aagcgcgcct
     6781 gaatgcgctg cctcatggcg cttcgcttta tttaccgatg gaagggctta acagctatcg
     6841 ccatgccatt gcgccgctgc tgtttggtgc cgaccattcg gtactgcaac aacagcgcgt
     6901 agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggcgcgg atttcctgaa
     6961 acgctacttt ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc
     7021 aatattcgcc ggggccggat tcgaagtaag tacttacccc tggtatgacg aagcgactaa
     7081 cggcgtgcgc tttaatgacc tgttggcgat gctgaaaaca ttacctgccc gcagtattgt
     7141 gttgctgcat ccatgttgcc acaacccaac gggtgccgat ctcactaatg accagtggga
     7201 tgcggtgatt gaaattctca aagcccgcga gcttatccca ttccttgata ttgcctatca
     7261 aggatttggt gccggtatgg aagaggatgc ctacgccatt cgcgccattg ccagcgctgg
     7321 attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt
     7381 cggcggactt tctgttctgt gtgaagatgc cgaagctgca ggccgcgtac tggggcaatt
     7441 gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc
     7501 tgcggtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg
     7561 tactcgcatt ctggcaatgc gtcaggaact ggtgaaggta ttgagcacag agatgccaga
     7621 acgcaatttc gattatctgc ttaatcagcg cggcatgttc agttataccg gtttaagtgc
     7681 cgctcaggtt gaccgactac gtgaagaatt tggtgtctat ctcatcgcca gcggtcgcat
     7741 gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt ttgctgcggt
     7801 gatgtaatgc aggaaagcag gctggagtta cccagcctgc agtgaaatta aactgtcgtc
     7861 gctttcactc tttctttata gatgattttt tgatgccatc gttctacgta agagataata
     7921 aacgttgtta gttcttttat tgttaagctt atcccaatta tctggaattc cttatcctgt
     7981 ttttttgtgt ggagtaataa tttctgcaca tctaaccatt attaaattta ataataagta
     8041 tcgggtttgt tacg
//
LOCUS       DAZGCQ010000182         8036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000182 DAZGCQ010000000
VERSION     DAZGCQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.182"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(166..1023)
                     /gene="murI"
                     /locus_tag="ACHK07_004821"
     CDS             complement(166..1023)
                     /gene="murI"
                     /locus_tag="ACHK07_004821"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO8105038.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(968..2851)
                     /gene="btuB"
                     /locus_tag="ACHK07_004822"
     CDS             complement(968..2851)
                     /gene="btuB"
                     /locus_tag="ACHK07_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO8105039.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     regulatory      complement(2916..3106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3220..4320
                     /gene="trmA"
                     /locus_tag="ACHK07_004823"
     CDS             3220..4320
                     /gene="trmA"
                     /locus_tag="ACHK07_004823"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO8105040.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMITGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4360..4719)
                     /gene="yijD"
                     /locus_tag="ACHK07_004824"
     CDS             complement(4360..4719)
                     /gene="yijD"
                     /locus_tag="ACHK07_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO8105041.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4719..5369)
                     /gene="fabR"
                     /locus_tag="ACHK07_004825"
     CDS             complement(4719..5369)
                     /gene="fabR"
                     /locus_tag="ACHK07_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO8105042.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5473..6789)
                     /locus_tag="ACHK07_004826"
     CDS             complement(5473..6789)
                     /locus_tag="ACHK07_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO8105043.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6832..7998)
                     /locus_tag="ACHK07_004827"
     CDS             complement(6832..7998)
                     /locus_tag="ACHK07_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO8105044.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2148 a         2165 c         1848 g         1875 t
ORIGIN      
        1 agcatttatt tttgctttct ctgccggaat tctcaggaga accccgctga cccggcggcg
       61 tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc ggcctgacaa
      121 gaggaaattt aaaataattt tccgaccgcg caacattcaa ccaaatcagc ctaaaactgc
      181 cagtttttcg agcgtttcga agccgtaacg ctgtaaaacg ggcaataatt gttcagcttc
      241 tggcgtcatt gccatacaaa aggcaatatt cgcatcggca gattttgcat ccggggcttc
      301 atgttctaac aaccaagccg ttcggcgagc aatcgctgcg ccagaatcca ccagccgggt
      361 tccctctggc agcacttgta acagttcttc ttgtagtaga gggaaatggg tgcaacccaa
      421 tacaacggta tctggcggct ctttcattct taaccacggg cgtaggatac gttttagtgc
      481 atccagagaa acatcttcgc catgcagctt cgcttcagcc aactcaacca tctctgccga
      541 gcccagcatt tctatctggc attcattagc gaaacgcgcg atcagctcat gagtataaga
      601 acgtttaact gttccgcggg ttgccagtaa tccgacaatg ccatttgccg tcagacgtgc
      661 agcaggttta atcgccggaa cgacaccaac aaccgggaag tcgaactttt cgcgtaaggc
      721 aggaagtgaa acggtactgg cggtgttgca agcgaccaca gccagcgcaa ggggataacg
      781 ttcttgcacc gcggtgacaa ttgccaccac tcgctcaaca ataaacgctt cgcttttttc
      841 gccatacggg aaagcgacgt tatcgaaagc ataaatgtaa tggagatccg gtaagagatg
      901 ccggatctcg tcatagaccg acaacccacc gacgccggag tcaaacacca gcacggtggg
      961 acgtggttca gaaggtgtag ctgccagaca aggtgtattc ccgtcctgca gtttggtagc
     1021 catagactgt ctcataatct ttgtcgaaca ggttggctat tttaccacga actgtcaggt
     1081 gagaggtgac cggatatgca accgcaagat cccacaagct cacaccgcct aatttaactg
     1141 tatgtgatga gccagaggtc tcgtcataag cgttgtcgta tcgttgacct atatattggt
     1201 aagaaacgtc ccattcgaaa ccataagctt gtccagtcaa ctcgtactta acctgctgtt
     1261 ttgcccgacg gtacagcact ttatctgttt catcatcgcg cgggtcaaca tattgtaacg
     1321 tcaagcgatg ttcaacagga ccggttgtaa tatttcccgt ccattccagc cctttaatcg
     1381 tggctgattt cacattaatg tactggttga cattatttat gcttttataa tcaatcaggt
     1441 tctgaatttc ataacgatat cctgagagcc gccaatccag agtcccagtt accccctcaa
     1501 ttccaatctc ccactgttta gattcttctg gcttcaaatt tgggttggct gcaataccag
     1561 gaccataaat tgaagcaaag cgagcagccc cgtattgctg tccaagagaa ggcgaaagaa
     1621 aactggtacc gtaagaaagt gttgcctggt agccatcaat aaactcccag ccagcagctg
     1681 tctgccaggt accatgccag ccaaactgtt catcatgatc ttctcggcca gaagcttcca
     1741 aagtcacatt ggaaatctgt tgctgtccag tcaggtataa cccggtggta tcgcgttcgt
     1801 aatcatctga ttttgtaatg ctgctggacg tcagtttttc ctgtttccaa tcgatcccgc
     1861 cactaattgc accatgccct acctcaatgc tattacccca ctgaatataa cgctgttcca
     1921 tgtcatccag ggtggcatct cccgcataac gaccaagatt atttgtgtag ttataattct
     1981 tgagtcgttg atagttagca attaactgag acgaatagat gccggagtta tagcgtaggc
     2041 cagtatccca ggactggttg taattctgag cttcttcgtt gccagtagca gaaccataca
     2101 tcgagccctg gtcgtaatca ttattagctg tgtagctgta accacggaag aaccccgaga
     2161 agttatcgtt aaacttatgc tgcagccccc cccagaaaag tttgttgcga tatccatcgc
     2221 gatcgctatc accactgtat ggtgagccag gctgaatgtt aaaaccttta gtcgtctggt
     2281 aagcacctgc tgctgttacc atcgtatcgc caaaacgttt atttattacc ccatcatagg
     2341 cttgataacc atttgtaccc attcctgcgt tgatctgagc tttctcatca ttactcattg
     2401 taataatgtt caccacacct ccgatggcac cagaaccata aacggcagaa cgcggcccac
     2461 gaatatattc aacacgctgt acaagtgata ccgggaactg gctaatatca attgcattgt
     2521 cgataccggc acgagccatc ggcacaccgt caatcaacac taaaacatgg cgcgattcag
     2581 tgccacgaac ataaagagaa gaactcgtcc ccattccacc agtttgagag atatttacac
     2641 cggggagatg actcataaca tcgttgaggt ctttcgactg ccagcgttga atatcctcac
     2701 gggtaacaat atcagtaggt gcaaggacag tatttactgg ctgctgaaaa cggttagcag
     2761 taacgacgag agtatccggg ctggtatcct gtgcccaagc ggaaaaggct gtgacagaac
     2821 acgccgtcag cagcgaagct tttttaatca ttgtaaagca tccacaatag aagaaggatg
     2881 ccgcaggttt catcaatatt acgcgatgat gagaaccaga tgcgacgtta gccggcaggt
     2941 cttcgggctt ggaggggtat ctaagatact aagatatgat gacttcccac cgaatggcag
     3001 tgtccgcata acgcaatcat cgcacctttc cttaccgctg cgcgtcagct ccagattcgc
     3061 actggattcc ctattaactc acaggaccgg caagtggatg ctacaggttg taacaagtta
     3121 ctgtccagac gtagctcaca aataggaatt catcaagatc tggacatctg atgagcaatc
     3181 cctacaatcg ccgcgtactt taatttttca ggatacatca tgacccccga acaccttcca
     3241 acagaacagt atgaagcgca gttagccgaa aaagtggtac gtttgcaaag tatgatggca
     3301 ccgttttctg acctggttcc ggaagtgttt cgctcgccgg tcagtcatta ccggatgcgt
     3361 gcggagttcc gcatctggca tgatggcgat gacctgtatc acatcatttt cgatcaacaa
     3421 accaaaagcc gtatccgcgt ggatagcttc cccgccgcca gtgaactcat caaccagttg
     3481 atgacggcga tgattacggg tgtgcgtaat aatcccgttc tgcgccacaa gttgttccag
     3541 attgattacc tcaccacgct gagcaatcag gcggtcgttt ccctgctata ccataaaaag
     3601 ctggatgatg agtggcgtca ggaagcggaa gccctgcgcg atgcactgcg agcacagaat
     3661 ctgaatgtgc atctgattgg tcgggcaacg aaaaccaaaa tcgcgctgga tcaagattac
     3721 atcgacgaac gtctgccggt cgcagggaaa gagatgatct accgtcaggt agaaaacagc
     3781 tttacccagc cgaacgcggc gatgaatatt cagatgctgg aatgggcgct ggacgtaacc
     3841 aaaggctcaa aaggcgattt actggagctg tactgcggca acggtaactt ctcattagcc
     3901 ctggcgcgca attttgatcg ggtattagcc accgaaatcg ccaagccgtc agtcgctgcc
     3961 gcgcaataca atattgccgc taaccatatt gataacgtac aaattattcg tatggcggca
     4021 gaagaattta ctcaggcgat gaatggcgtg cgcgagttta accgcctgca agggatcgac
     4081 ttaaagagtt atcagtgcga aaccattttt gtcgacccac cgcgcagcgg tctggacagt
     4141 gaaaccgaga aaatggtgca ggcgtatcca cgtattctgt atatctcctg caatccggaa
     4201 acgttatgca agaatctgga aacattaagc cagacgcaca aggtcgaacg tctggctctg
     4261 tttgatcagt tcccctacac gcaccatatg gagtgcggcg tattactgac cgcgaagtaa
     4321 aacccatgcc ggatgcgcca gcatccggca tgataccgat tactcagcaa cttcctgctt
     4381 acggttacgc atcttcgcgc caatccagaa caccataatg acggagagta ctgccgggaa
     4441 gaagttagag ccgatatccg gatattccgc acgaactacc gtgctgtaca gcagtacgcc
     4501 gagaataaaa caggcagctg ccagacccgg caagcctacc ggcatggtgc gattaagata
     4561 acgttgatgc aggcagtaaa ccgtcagcac cagggaaata atcgggaata cagaaaatgg
     4621 cacaatggag ctaaacagcg ctgcgaaagt accattaatc gataagccag caaccaacgc
     4681 cagcagcagc gtacctctat cttgatttgc ttgtttcatt actcgtcctt cacatttccc
     4741 ggaataattg cggttttctc ttgttcacgg cgataccagt aataagcccc tttcgaaatc
     4801 attcgcagtt gcagtaccag tcgctcttct aattgccgac gttgttcgac gccgacgtcc
     4861 aacgcctcgg cacccgcact gaagacaatt gtcaccattg cttcggcttg cgcttcagta
     4921 aacgcacgcg gcatatggtt ttcgagttcc agatagtccg caagttccgc aatgaagtgc
     4981 tgaatttcac gcgcaacggc ggcacgaaac gcagcggagg tgccggagcg ttcccgcaat
     5041 aataaccgga aggcgttagg attattaccg atgaactcca taaatgtgga gaccgaggta
     5101 cggatcacac tcccgccttt ggcgatacgc tgacgcgcct ggcgcatgag ttggcgtagc
     5161 attaaaccgc tctcatcaac catggtcaga cccagttcgt ctacgtcgcg gaaatgccga
     5221 taaaaagagg tgggagcaat gcccgcttca cgcgccactt cacgcaaact caggctggcg
     5281 aagctgcgtt cagcacttaa ttggctaaat gcggcttcca ccagcgaacg gcgggttttt
     5341 tctttttgtt gcgctcttac gcccatcacg atgtctgaat ccttgccaaa agtactgctg
     5401 gcactatacc agagaatgaa catactggat tgctccagta ttgtgaatga acggtaacgc
     5461 aataaaacat atttactgca attctttggc cggttctttt acgtacaaca acgccaacat
     5521 cgacagcaac gtgcctccac ccaaatacca cgccggagcc gtcaaactat ccagcaacac
     5581 gatggattgc gtagcgaaat attgagcaaa accgccaaag attgccacac caatgctata
     5641 aaccagcgca aaacccaatg cccgaatccg tttcggcaac agttcagaga tcaacaacat
     5701 cactggcaca ccgccaagcg tggtaaagct gaccatcacg aaaaccaccg tcagcaacat
     5761 gccagggctt gggtaattca ccaacagcca gaatgacggc cagctacaga tcagcaccat
     5821 cacacgggag attaaaatca gctttttacg cccgaccgaa tcgcacaaca tccccaccag
     5881 cagcgcaccc acaaacgtaa taacgcccgc cagtaacatc gctgcatgag aatagttttg
     5941 gttcattcct aaatacttcg ccgcccatgt accgtaataa aagagggaga tataggttgc
     6001 gactgtacta ccaatagcga gcaagatacc gttagcgata gtcgccttat gttgcaggag
     6061 cagagaaaac gcgctttcgc tggtagcagc ttttttattg cgcacaggct caggtacgtc
     6121 attttccaga ctcaaacgca accaacagcc gacaggcgct aataacacgc caataaaaaa
     6181 cggcacacgc cagccccatt cagccatgac ggtttctgaa ccggtcgcaa agggcagcca
     6241 tgcacttaaa cctaaagcga caacacctcc aaaagttgtt gccaggcctt gagtcgccag
     6301 tgaccaactg ctgtaaaaac cgcgacgatt tgccggagct gactccacca gtagcgacat
     6361 tgaagcccca acttcaccgc ctgctgcaac accctgaatc aaacgagcaa gtaccagagt
     6421 tgccgttccc caatagcccg ccgtcgcata cgtcggcgca atgccaatca tcaacgtgcc
     6481 gaggctcatc agggcaatag tgaacaccat cgcgggtttg cgtccgaaac gatcggccca
     6541 ggccccgacg ataattccac caagcgggcg cataaaaaaa ctcacgccaa acgttgcaaa
     6601 tgccagtaac agcgcgacat tgtgatcagc gctgggtggg aagaaaagtg tactgatata
     6661 gaccacaaaa aagttgtaaa cgccaaaatc aaaaaattcg agcgcattgc caaacgccga
     6721 ggcaaaaatc acccgcctgc cgctgggctt tttcttcaca ctcacggttt catttacgat
     6781 cgccgtcatt ccatgttcct tgccaaaaag tgttgttgtg tgttgtttta tttattgcag
     6841 ccaggcttct accagtgcgc cccagtagct gcttgccggg accagacttg catcgttgaa
     6901 gtcgtaaccc gggttatgca ccatgcagga accttccccg acatcgccat taccgaataa
     6961 caggaaaccg cctttgggtt gggcttccag catgaaagag aaatcttcac tgcccatcag
     7021 aggcttgatg ccaaactcgg cccgatcttc accaaacgtt ttacacgcca cctgccaggc
     7081 gaagcgcgcc atctcctcgt catttactaa tactggtgta ccgttaacat gcgtaacctc
     7141 agccgtagcg ccaaagctgg cagcctgggc ttgtgccaga gcaggaatac gggtcagaag
     7201 aagttgtcgg gtatcgcggc taagagagcg gacgctgatt ttcatctcag cactgtcagg
     7261 aatgacgtta gccgcctcac ccgcaacaat gctgccgacg gtaattaccg ccgcttccag
     7321 cggatcgaca ttgcgtgaca caatgctttg taatgcggtg gtgatatgtg cggcaaccag
     7381 caccggatcg atggctttgt gcgggatcgc accgtgtccg ccacaaccac gaaccgtaat
     7441 atggaactga tccattgacg ccatcagtgc ccctggctgg aaataaaact tacccaccgg
     7501 cagaccgggc atgttatgca tgccgaaaat gacatcgcag gggaaatgat caaaaagccc
     7561 ctctttaacc atgatctcac cgccattaat catctcttcc gcaggctgga agataagacg
     7621 taacgtgccg ttaaaacggc gggtttcggc aaaataacgc gcggccccca gcagtatggt
     7681 ggtatgcccg tcatgcccgc aggcgtgcat taaaccagga tgtttactgg cccagagctt
     7741 gccactattt tcataaattg gcagcgcatc catatccgcc cgcaggccaa tacttttctc
     7801 tccgtcgccc acttttaacg tggccactat ccccgtcgtc gccagtcctt gctctacttc
     7861 atagccccac tgacgcagct tttccgcaac caaagcactg gttttaaatt cttgtaatcc
     7921 cagctccggg ttttcgtgga tctgatggcg gatctcacgc agttcttcct caaaccctcg
     7981 aatatagtgt tcgatcatcg gctattcctt tattcactta taaacaggct aattaa
//
LOCUS       DAZGCQ010000183         7615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000183 DAZGCQ010000000
VERSION     DAZGCQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.183"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(187..450)
                     /gene="cedA"
                     /locus_tag="ACHK07_004828"
     CDS             complement(187..450)
                     /gene="cedA"
                     /locus_tag="ACHK07_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO8105045.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            633..2894
                     /gene="katE"
                     /locus_tag="ACHK07_004829"
     CDS             633..2894
                     /gene="katE"
                     /locus_tag="ACHK07_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO8105046.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAIILNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2941..3699)
                     /gene="chbG"
                     /locus_tag="ACHK07_004830"
     CDS             complement(2941..3699)
                     /gene="chbG"
                     /locus_tag="ACHK07_004830"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO8105047.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGVVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3712..5064)
                     /gene="celF"
                     /locus_tag="ACHK07_004831"
                     /gene_synonym="chbF"
     CDS             complement(3712..5064)
                     /gene="celF"
                     /locus_tag="ACHK07_004831"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8105048.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5168..6010)
                     /gene="chbR"
                     /locus_tag="ACHK07_004832"
     CDS             complement(5168..6010)
                     /gene="chbR"
                     /locus_tag="ACHK07_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO8105049.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6018..6368)
                     /gene="chbA"
                     /locus_tag="ACHK07_004833"
     CDS             complement(6018..6368)
                     /gene="chbA"
                     /locus_tag="ACHK07_004833"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO8105050.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6419..>7615)
                     /gene="chbC"
                     /locus_tag="ACHK07_004834"
     CDS             complement(6419..>7615)
                     /gene="chbC"
                     /locus_tag="ACHK07_004834"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO8105051.1"
                     /translation="FGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMA
                     PFFIGMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIG
                     LVVAEMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNF
                     HQIIMDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIA
                     TYQQYGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVA
                     KLALPSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIA
                     PWTMPTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDI
                     ANALKF"
BASE COUNT         2052 a         1997 c         1699 g         1867 t
ORIGIN      
        1 ctcatcatga ttagcaaaac ttaaccattt taaaataaat aaacaattaa agaaaaaaga
       61 tcacttattt atagcaatag atcgtcaaag gcagcttttt gttacaggtg gtttgaatga
      121 atgtagcaat gaaatacaaa atttcaggcc atgtaactcc cggcaaaacc gggaggtacg
      181 taatccttac tcagtcactt ccccttcctg gcggatctga tttgcccaac gctgggcgga
      241 ttcaggcaca gtaaacgccg gtgagcgcag aaatgactct cccatcagta caaacgcaac
      301 atatttgcca cgcagcatcc agacatcacg aaacgaatcc atctttatcg catgttctgg
      361 cggcgcgggt tccgtgcgtg ggacatagct aataatctgg cggttttgct ggcggagcgg
      421 tttcttcatt actggcttca ctaaacgcat attaaaaatc agaaaaactg tagtttagcc
      481 gatttagccc ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtc
      541 tccgaagcgg ggtctggctg gtggtctata gttagagagt tttttgacca aaacagcggc
      601 cctttcagta ataaattaag gagacgagtt caatgtcgca acaaaacgaa aagaacccac
      661 atcagcacca gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg
      721 cacctgagga cggatctcat cgtccagcgg ctgaaccaac accgccaggt gcacaaccta
      781 ccgccccagg gagcctgaaa gcccctgata cgcgtaacga aaaacttaat tctctggaag
      841 acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa tcagggtgtg cgcatcgccg
      901 acgatcaaaa ctcactgcgt gccggtagcc gtggtccaac gctgctggaa gattttattc
      961 tgcgcgagaa aatcacccac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac
     1021 gtggatcagc agctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag
     1081 cggatttcct ctcagatccg aacaaaatca ccccagtatt tgttaggttc tctaccgttc
     1141 agggtggtgc tggctctgcc gataccgtgc gtgatatccg tggctttgcc accaaattct
     1201 ataccgaaga gggtattttt gaccttgttg gcaataacac gccaatcttc tttatccagg
     1261 atgcgcataa attccccgat tttgttcatg cggtaaaacc agaaccacac tgggcaattc
     1321 cacaagggca aagcgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc
     1381 tgcacaacgt gatgtgggcg atgtcggatc gcggcatccc ccgcagttac cgcaccatgg
     1441 aaggcttcgg tattcacacc ttccgcctga ttaatgccga agggaaggca acgtttgtac
     1501 gtttccactg gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac
     1561 taaccggacg tgacccggac ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg
     1621 attttccgga atacgaactg ggcttccagt tgattcctga agaagatgaa ttcaagttcg
     1681 acttcgatct tctcgatcca accaaactta tcccggaaga actggtgccc gttcagcgtg
     1741 tcggcaaaat ggtgctcaat cgcaacccgg ataacttctt tgctgaaaac gaacaggcgg
     1801 ctttccatcc tgggcatatc gtgccgggac tggacttcac caacgatccg ctgttgcagg
     1861 gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg
     1921 agattccgat taaccgtccg acctgccctt accacaactt ccagcgtgat ggcatgcatc
     1981 gtatggggat cgacactaac ccggcgaatt acgaaccgaa ctcgatcaac gataactggc
     2041 cgcgcgaaac accgccggga ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg
     2101 aaggaaataa agttcgcgag cgcagtccat cttttggcga atattattcc catccgcgtc
     2161 tgttctggct aagtcagacg ccattcgagc agcgtcatat tgtcgatggt ttcagttttg
     2221 agttaagcaa agtcgttcgt ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata
     2281 ttgatctcac tctggcccag gcggtggcga aaaatctcgg tatcgaactg actgacgacc
     2341 agctgaatat caccccacct ccggacgtca acggtctgaa aaaggatcca tccttaagtt
     2401 tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt agcgattata cttaatgatg
     2461 aagtgagatc ggcagacctt ctggccattc tcaaggccct gaaggccaaa ggcgttcatg
     2521 ccaaactgct ctactcccga atgggtgaag tgactgcgga tgacggtacg gtgttgccta
     2581 tagccgctac ctttgccggt gcgccttcgc tgacggtcga tgcggtcatt gtcccttgcg
     2641 gcaatatcgc ggatatcgct gacaacggcg atgccaacta ctacctgatg gaagcctaca
     2701 aacaccttaa accgattgcg ctggcgggag acgcgcgcaa gtttaaagca acaatcaagg
     2761 tcgctgacca gggtgaagaa gggattgtgg aagctgacag cgccgacggt agttttatgg
     2821 atgaactgtt aacgctgatg gcagcacacc gcgtgtggtc acgcattcct aagattgaca
     2881 aaattccggc gtaaatatgg ataaggtcac gtcccctggg gagtgacctt accaccttac
     2941 ttacacatca agataactac ccaaccgata accacgctca gcaattgcat atttcaacga
     3001 cgctgatgtc agcacttcca gctccgttaa ccgtgggaaa cagtaagcac tctggcgaat
     3061 cgtattatcg acaaacgccg gatggcacat gacttccagc gagcgttccc cgcgatggct
     3121 ggaatcatca agcacttgca ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct
     3181 actgaatccg tgcgaactgc gcagattagc cggtaaatca ccagcgttag atagcggctg
     3241 acggtcgatg cgcaacgcaa tcccctcttc agccgcaaac ctggcaacaa tagggaaaat
     3301 ctgcgggaac atatgcacat gatgatggct atcaagatgc gtaggtttgc gtccaaatag
     3361 ctcaatgaaa cgcaaatact gactggcaag ctcctgagta atttcttcca gcggtaaagc
     3421 atcttcttct gccaactgcc agatccattt tcccagcaca ccatcgcggg ttaaccccgg
     3481 catagctgtc agtggcttgc ccatagtaag gacaaagtgc atccctatgg ccagactcgg
     3541 ttcatcacga ctcaactgca ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt
     3601 cgatgtgaca accccattgc gacaagcctc gataatgccg tagttctgac ctttgcttaa
     3661 gccaaaatca tcggcattaa caatcagtaa gcgttccata atcagcctcg gttaatgtgc
     3721 ttttttaagc tctgcgatgc agtcggcaaa atttggcagc catttctcgt gcgccagaat
     3781 catctcgcgt gccagcagct cagcatcgcg atcggaatgc accaacggac taaggtttag
     3841 cgccagtaac acatcgttaa attctccgct aagtgcggcg ttgctggcag caatctcgaa
     3901 gcctttaatg gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg
     3961 tggcgtcgcg ccatcgcgcc ccagcgtaca ggtcatttct accgcccagt ctgccggaat
     4021 attatcaata tgcccatgat gcgggatatt aacgtaatgt tctgcttgct tatcgttgta
     4081 gatagcgttg atcacttcgc acgctgcatc agagtaataa gccccaccgc gctgttccag
     4141 ttctttcggc ttaactttca actccgggtt tttatacagc tcaaaaagtt gtttctcgac
     4201 tttctgtact acctgcgctc gtgcgccgcc tttgtagtat tcgcccattt caatagccag
     4261 catctctttt tgcttgaagt aataaagcaa ataagaacac ggcaacagat tcagagaacg
     4321 aattaagcct tcgctaaatg gcagatcgaa aatattttta acgccagatg cttttaactg
     4381 cccggatgcc acaccatcaa gtaattcggc aaagcgagat ttgccattta ccagcacatc
     4441 cttaatgaac accatatggt tgaggccgaa cagatcgata gataaatcat cgctgtcttt
     4501 cagcatcaga acatcgcgaa taaacatctt catgccgatc ggaatattac acacgccgat
     4561 aaagcgttta aatccggtat gacgataaac ggcttcagtg accattcccg ccgggttagt
     4621 gaagttaatc acccatgcat tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat
     4681 caccggaatg gtacgcagac ctttaaacag accgcccgcg ccgttggttt cctgaccaag
     4741 ataaccatga cttaatggaa tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg
     4801 cagttgggta gtaacgaaat cagcatcttt caatgcttcg cggcgatcca gcgttttata
     4861 aagcttcatc gggacgccag cgttatcaat catccgttgg cagagatcga aaataatatc
     4921 cagtttagct ttgccacctt cgacatccac cagccataat tcgctgaccg gcaattcgtg
     4981 ataacgctta ataaatcctt ccagtaactc cggggtatag ctgctcccgc caccaatagt
     5041 gacgactttt aatttctggc tcataatttc tcccttcagt acagaatact gatatctgac
     5101 atatctgccc cccggacata aataatccag caacaggaca gatatgtgaa ttgtcaggta
     5161 taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc gtaaaggacg
     5221 ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc tcaaacgcaa
     5281 tatccgtcac tgaatagttg gtcatttcca gttgtttttt ggcaaaatta atacggattt
     5341 cattaataat ctgcattggc gttttgccat aatatcgttg agtcgctcgc gtcaaatatt
     5401 cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta aactgctctt
     5461 tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca atcacctgtt
     5521 cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca acaaactctt
     5581 ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag gtgagaaaag
     5641 cgttattggt ccggtatacc tgcgaagcta caaagcaata aggcaacaac ggcaggtaat
     5701 gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat acgcgtggca ccaaactcat
     5761 aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt tccagtaaca
     5821 cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta aattcataat
     5881 agtcgtgctg atgcagtccg ctgatactct cagttttgtt atagataaac acatggaagt
     5941 ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg ttaatcactg
     6001 gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa ttctgtaatc
     6061 agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag caccagactc
     6121 actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct
     6181 tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcggcaaa atcgccctgc
     6241 ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt gatgataagc
     6301 cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat attatcgaga
     6361 tccatcatat atcgttcctc tctattctta ccggcacgat tacccgtacc ggcatcgatt
     6421 aaaatttcag ggcgttagcg atatcttctt cgctctcttc tttatcaatc gcgttttgtg
     6481 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca aggttgaaga
     6541 gtgcaaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     6601 gcatggtcca cggtgcaata ttggtcaccg gaggaataat gcccatgtag tacgctgcca
     6661 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     6721 taataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     6781 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaaaata gccaggatca
     6841 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     6901 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     6961 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     7021 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     7081 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     7141 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     7201 cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     7261 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     7321 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     7381 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     7441 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     7501 tccctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     7561 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaa
//
LOCUS       DAZGCQ010000184         7610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000184 DAZGCQ010000000
VERSION     DAZGCQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.184"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHK07_004835"
     CDS             <1..285
                     /locus_tag="ACHK07_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8105052.1"
                     /translation="RSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPRKNI
                     RQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPR"
     gene            546..2168
                     /gene="yhiJ"
                     /locus_tag="ACHK07_004836"
     CDS             546..2168
                     /gene="yhiJ"
                     /locus_tag="ACHK07_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8105053.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFEKIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIILESLQ
                     PYLVINKGAVTPCTFLGDQTGDRFSNICGDQFIIDLLKCIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHTGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2358..2471
                     /gene="yhiY"
                     /locus_tag="ACHK07_004837"
     CDS             2358..2471
                     /gene="yhiY"
                     /locus_tag="ACHK07_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO8105054.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2534..3601
                     /gene="yhiI"
                     /locus_tag="ACHK07_004838"
     CDS             2534..3601
                     /gene="yhiI"
                     /locus_tag="ACHK07_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8105055.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3598..6333
                     /gene="rbbA"
                     /locus_tag="ACHK07_004839"
     CDS             3598..6333
                     /gene="rbbA"
                     /locus_tag="ACHK07_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO8105056.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6333..7457
                     /gene="yhhJ"
                     /locus_tag="ACHK07_004840"
     CDS             6333..7457
                     /gene="yhhJ"
                     /locus_tag="ACHK07_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8105057.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1898 a         1791 c         2020 g         1901 t
ORIGIN      
        1 agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa cactactgat
       61 ttcagaccag atcctgaaga tatcttcaaa tgtggtcaga cgctcgaatg tggcgacccc
      121 cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca gaatcaacaa
      181 tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga aaataaaaaa
      241 ataataggtt cttcctcagg gcttagttgt tttcaacctc gttaaaactc catgagtgct
      301 gtggtatgtt ttttaatcct gccagtattc cataggtttt cttaatcgat tgtagaggta
      361 tgtactgatg acgcctctac aatctttagg gactttctct actatgagat aagaaatttc
      421 atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc atcgaacatg
      481 aggtgtatgt cataagatta aatgataact atgtattcct cgcggtgaat tatatgaggt
      541 aaaagatgaa aattggaact gtggcaggta ctaacgacag tataacgaca atcgcaacaa
      601 atgatatggt gcaagagcat gttaccaact tcactaaaga actgtttggg tatattgcaa
      661 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgag gttgtggaaa
      721 aaatagatga ttggcaaata gaacggttcc aacaatctat tcaagatgac aaaatatcat
      781 ttaccataca aacggatcat tcagaaaaat atagtatgct atcgggcatg cgtgcccata
      841 ttctacgtag gaataatagt tatcagttta ttgtgacaat taatagtaaa aattacggtt
      901 gccctcttga tgataccgat attaattggt gcagcattgt ttatttactt aataatatga
      961 cagtaaatga taatgctaat gatgttgcag ttactgagtc gtacaaaccc atctggaact
     1021 ggaaaatatc gcaatacaat gtatctgata ttaaattcga aaaaattata aaaccccagt
     1081 tcgctgacag aacctatttc tccaattgtt cacctgttga tcccacaagt accagaccaa
     1141 cctattttgg tgataccgat ggctcagttg gtgcagtttt gtacgcgctt tttgcaacgg
     1201 gacatttggg aatcatggct gaaggagaaa atttccttag ccagttgctt aatatagaag
     1261 atgaagttct caatgtattg ctaagggaga actttaatga gcaacttgat actaatgtta
     1321 atacaataat cagtatcctg aacagaagag acattatttt agaaagtctt cagccttatt
     1381 tagttattaa taaaggcgca gtgacgccat gtacgttttt gggtgaccaa accggcgatc
     1441 gatttagtaa tatctgtggc gatcaattta tcatcgattt gttaaaatgc ataatgagca
     1501 tcaatgataa cgttcatgta ttagctggaa atcatgaaac gaattgcaat ggtaactata
     1561 tgcagaactt cacgcgtatg aaaccacttg atgaggatac ctacgctggg ataaaagatt
     1621 atcctgtatg tttctatgac tccaaataca aaataatggc aaatcatcat gggataactt
     1681 ttgatgatca gcgaaaacgt tacattatag gaccgataac ggtctctatt gatgaaatga
     1741 ccaatgctct cgatccggtc gaattagcgg caattattaa taaaaagcat catactataa
     1801 ttaatagtaa aaagtttaaa actagccggg ctatctcttg tcgatccttt aatcgttatt
     1861 tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgc tcacaaatgt
     1921 taggtataaa tcaagttgtt gcacataacg gtaatggtgg aagagaacgc ataggtgaaa
     1981 cggggacagt tttaggtctt aacgcacgtg acagtaaaca tacgggaaga atgttcagca
     2041 tgcataattg ccaaattaac cctggtgcgg ggcctgagat aaccactccc tggaagtctt
     2101 accaacatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc acaatgttac
     2161 agctctgaac catagacaac atgaatatta atgttactat ctggttgaga ctgttgataa
     2221 aacgtaataa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg
     2281 atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc gtgtttcatt
     2341 tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag ccttccccgt
     2401 tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc ccggatggaa
     2461 gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt acagggatta
     2521 tggcgggtcg attatggata agagtaagcg ccatctggcg tggtggggtg tcgggttact
     2581 ggcggtggct gctatcgtgg cgtggtggct gttgcgcccg gcaggtgtgc cggaaggctt
     2641 tgccgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca aaattgccgg
     2701 gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggcg aagtgctggc
     2761 gaagatggat actcgcgtgt tgcaggagca gcgactggaa gccatcgcgc aaatcaaaga
     2821 ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg
     2881 cgccgcacag tcgctggtta atcaacgcca ggccgaactg gactccgttg ccaaacgtca
     2941 tacgcgttcc cgctcgctcg cccaacgagg ggctatttct gcgcaacagc tggatgacga
     3001 tcgcgccgcc gctgagagcg cccgagctgc gctggaatca gcaaaagccc aggtttcggc
     3061 ttctaaagcc gctatagaag cggcacgcac caatatcatt caggcgcaaa cccgcgtcga
     3121 agccgcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg aactgaaagc
     3181 cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc tggcggcagg
     3241 cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct tcctgccaac
     3301 cgaacaggcg ggcacgctga aacttggcgg tgaagcccgg ctgatcctcg atgccgcgcc
     3361 agatctgcgt attcctgcaa ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa
     3421 aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc
     3481 accggaatta ctccagcagc atctggaata tgtcaaaacg ggcttgccgg gcgtagcgtg
     3541 ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg
     3601 acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat
     3661 tatggaaaaa ccgttgcgct gaacaatatc acactcgata ttccggcccg ctgtatggtc
     3721 gggctgattg gtccggatgg cgtcgggaag tcgagtttat tgtcattgat ttccggtgct
     3781 cgtgtcattg agcagggcaa tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat
     3841 cgccgtgacg tttgcccgcg catcgcctgg atgccgcagg ggctgggcaa aaatctctac
     3901 cacaccttat cggtgtatga aaacgtcgat tttttcgctc gcctgttcgg tcacgacaaa
     3961 gcggagcggg aagtccgaat taatgagctg ctgaccagca ccgggttagc accgtttcgc
     4021 gatcgtccgg cagggaaact ctccggcggg atgaagcaaa aactggggct gtgctgtgcg
     4081 ttaatccacg acccggaact gttgattctt gatgagccaa caacgggggt tgacccgctc
     4141 tcccgcgccc agttctggga tctgatcgac agtattcgcc agcggcagag caatatgagc
     4201 gtgctggtcg ccaccgccta catggaagag gccgaacgct tcgactggct ggtagcgatg
     4261 aatgccggag aagtgctggc aaccggcagc gccgaagagc tgcggcagca aacgcaaagt
     4321 gccacgctgg aagaggcgtt tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag
     4381 gcggtagtga tcccaccata tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat
     4441 ctgaccatgc gttttggttc cttcgttgcc gttgatcacg ttaatttccg cattccacgc
     4501 ggggagattt ttggttttct tggttcgaac ggctgcggta aatccaccac catgaaaatg
     4561 ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc tgtttgggca accggttgat
     4621 ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat
     4681 aacgaactca ccgtgcggca aaacctggag ttacatgccc gtttgtttca catcccggaa
     4741 gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa
     4801 gatgttctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct ggcggtggcg
     4861 gtgattcatc gcccggaaat gttaatcctc gatgagccta cttctggcgt cgatccggtg
     4921 gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa agtgaccatt
     4981 ttcatctcca cccactttat gaacgaagcg gaacgttgcg accgtatctc actgatgcac
     5041 gccggaaaag tgctcgccag cggtacaccg caggaactgg ttgagaaacg cggagccgcc
     5101 agtctggaag aggcatttat cgcctatttg caggaagcgg caggacagag caacgaagcc
     5161 gaagcgcctc ccgtgataca cgatgctaca cacgcgccgc gccagggatt tagcctgcgc
     5221 cgtctgttta gctacagccg gcgcgaagca ctggaattgc gccgcgatcc ggtccgttcg
     5281 acgctggcgc tgatgggaac ggtgatcctg atgctgataa tgggttacgg catcagtatg
     5341 gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag tagtcaggcg
     5401 tggacgctca acctctccgg ttcccgttac tttatcgaac agccgccgct taccagttat
     5461 gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg tggcaattga gatccctcca
     5521 aatttcgggc gtgatatcgc gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga
     5581 gcgatgccca gtcgtgccga aacggtaaaa ggttacgtgc aggctatgca ccagagctgg
     5641 ctacaggatg tggcgagccg acaatccaca ccctccagcc aaagcgggct gatgaacatt
     5701 gagacgcgct atcgctataa cccggacgta aaaagcctgc cagcgattgt tccggcggtg
     5761 atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagtgt ggtgcgggaa
     5821 aaagaactag ggtcgattat caacctttac gtgactccca ccacccgtag cgaatttttg
     5881 cttggcaaac agctaccgta catcgcgctg gggatgctga actttttcct gctctgcggt
     5941 ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct caccctggcg
     6001 gcgctgctgt atatcatcat cgccaccgga atggggctgc tgatctccac ctttatgaaa
     6061 agccagattg ccgccatttt cggtacggcg attatcacgt taatcccggc gacacagttt
     6121 tccgggatga tcgatccggt agcttcgctg gaaggtccag gacgttggat cggcgaggtt
     6181 tacccgacca gtcattttct gactatcgcc cgcggaacgt tctcgaaagc gttggatctg
     6241 actgatttat ggcaactttt tatcccgttg ctgatagcca taccgctggt gatgggctta
     6301 agcatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat atttttaatc
     6361 tgggtatcaa agagttgcgc agtctactcg gtgataaagc gatgctgaca ttgattgtct
     6421 tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg ttgaacctgg
     6481 caccgatcgc cattgccgat atggatcaat cgcagttatc gaatcggatc gttaacagct
     6541 tctatcgccc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg gatgccggac
     6601 tggacgccgg acgctatacc ttcgcgataa atattccgcc taattttcag cgtgatgtcc
     6661 tcgctggacg ccagccagat attcaggtga acgtcgatgc cacgcgcatg agccaggcat
     6721 ttaccggcaa cgggtatatc cagaatatta tcaacggtga agtgaacagc tttgtcgcgc
     6781 gctaccgtga taatagcgag ccgttggtat cgctggaaac ccggatgcgc tttaacccga
     6841 acctcgatcc cgcgtggttt ggcggggtga tggcgatcat caacaacatt actatgctgg
     6901 cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg gtggaacact
     6961 tactggtgat gccgataacg ccgtttgaga tcatgatggc gaaggtctgg tcgatggggc
     7021 tagtggtgct ggtggtatcg ggattatcgc tggtgctgat ggtgaaaggc gtgctgggcg
     7081 taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca
     7141 ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa ctggggctgc
     7201 tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggatccacg ccgcgcgaaa
     7261 gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac tttgttagcc
     7321 tcgcgcaggc cattctctac cggggtgccg gattcgaaat cgtctggccg cagttcctga
     7381 cgctgatggc aattggcggc gcgtttttca ccattgcgct gctgcgattc aggaagacga
     7441 ttgggacaat ggcgtaaata caattgcccg atgcgtaagc ttatagggac tacgcatcgg
     7501 tgcaatttat tgaattttag ttgtttttag gccggataag gcgtttatga aatgaccccc
     7561 aaaacctgga cagtttcagt taaccggctt tttgatactc agtccggtat
//
LOCUS       DAZGCQ010000185         7598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000185 DAZGCQ010000000
VERSION     DAZGCQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.185"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(195..557)
                     /gene="yibL"
                     /locus_tag="ACHK07_004841"
     CDS             complement(195..557)
                     /gene="yibL"
                     /locus_tag="ACHK07_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO8105058.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
     gene            842..1051
                     /gene="yibT"
                     /locus_tag="ACHK07_004842"
     CDS             842..1051
                     /gene="yibT"
                     /locus_tag="ACHK07_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO8105059.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1063..1650)
                     /gene="mtlR"
                     /locus_tag="ACHK07_004843"
     CDS             complement(1063..1650)
                     /gene="mtlR"
                     /locus_tag="ACHK07_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO8105060.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPHFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1650..2798)
                     /gene="mtlD"
                     /locus_tag="ACHK07_004844"
     CDS             complement(1650..2798)
                     /gene="mtlD"
                     /locus_tag="ACHK07_004844"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8105061.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3028..4941)
                     /gene="mtlA"
                     /locus_tag="ACHK07_004845"
     CDS             complement(3028..4941)
                     /gene="mtlA"
                     /locus_tag="ACHK07_004845"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO8105062.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMFLAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGHK"
     gene            5478..5840
                     /gene="yibI"
                     /locus_tag="ACHK07_004846"
     CDS             5478..5840
                     /gene="yibI"
                     /locus_tag="ACHK07_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8105063.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5843..6979
                     /gene="yibH"
                     /locus_tag="ACHK07_004847"
     CDS             5843..6979
                     /gene="yibH"
                     /locus_tag="ACHK07_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8105064.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(7068..7274)
                     /locus_tag="ACHK07_004848"
                     /pseudo
     CDS             complement(7068..7274)
                     /locus_tag="ACHK07_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(7440..>7598)
                     /locus_tag="ACHK07_004849"
     CDS             complement(7440..>7598)
                     /locus_tag="ACHK07_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HFO8105065.1"
                     /translation="ADCIETVNKIKDKNKKIIDINSVNICSDDKNIKWRVNSTIMAID
                     DSIKCIDE"
BASE COUNT         1877 a         1885 c         1848 g         1988 t
ORIGIN      
        1 cttctatata tttttattgt ttataggttg ctatttcggt aaaaatatat tttcagaata
       61 tcaaccagcg gtaaattgat gtagagggag tggattgttc agtaattagg cgagcagaca
      121 ggggagtccc cgcattgacc agctaacgca agccagatgc gggttgatac gatcgtacca
      181 atcgtctgag gggcttaaaa cgcagtctta gagaatccag tcatctcttc caggcccatt
      241 tcgcggccca gcgcggtcat tgggtgaaca accaccagtc cgcgaacact tttcttcagc
      301 ttgcccatat cagcctgctc ttttttggta attgcgcgct gaaacggcat cttcatcagc
      361 ttctgtgctt ctttactcag tttctggctg tgaacttcac gcaggcgggc aatttctgct
      421 tccagcgtgg ctttctcttt ttccagctcg gcatatttgt ctgcggcatc aaccagcgac
      481 agatcggcct gctggtggcg gatggcgtcc agacgatcgc tgagacgttt gatttcgttt
      541 ttttcgactt ctttcatgtg atggcttctg ataaagataa atttatagcc cctagtatgc
      601 gcgaccaggt gctttgttca caactgatga cattgcaata gttccgcaac gtaatcatta
      661 atatgcttaa aaactacgca atttattgat taattaagaa atctatagaa aaaagataat
      721 aaacagaagc ggatgattat tgaggattca gcacgttttc cttaatgtca tctttaccgg
      781 gaacctaaac taaaattggc ggccgcgcat tcccggcgcg gggtaatacg gagatatcat
      841 catgggcaaa ttaggtgaaa acgttccgct tcttatcgat aaagccgtag atttcatggc
      901 atcaagccag gcgttccggg agtatctgaa aaaacttcct ccccgtaacg cgattccgtc
      961 cggaataccc gatgaaagcg tgccgttata tctacaacgt ctggagtatt atcgtcggct
     1021 ttatcggccg aagcaggtag aggggcagta agcagacgtt gcttactttt gaaaggcttt
     1081 ttttaagctg attttggaaa tcagctctgt cagtgaaagg accattgttg atcgcacagc
     1141 ctgttgatag cgctgaattt gcattgcata caaactggag tctgctggtt caaaatgtgg
     1201 cggcggtggt aacgccgcca cgcagtgcag ttcaccaaag ggtccaagga tttcgtcgtc
     1261 ggtaaacgcg tactcgttgc cgtcgtgatt tagctcttca cgcaatgcca tcagcagttc
     1321 cgcatcttcg tattcctggc ggttaatgac gcccaacccg taaatgagtt ttaaacgcac
     1381 agaaagatcg cccagcggac cgtcgccgtc gagtaacggt tctacagcat acttcaccgc
     1441 gtaatcgtct ttgcggaata cctgaagcac cagaagattt accgcctcgg tcaaaagctc
     1501 gacggcggtg atcagaaagc ttcgcacggt tttgccagca ttcagacgct caagcacacg
     1561 gttttcaaag gcctgggttt gttccattgt tgcctgcata tctgacaatc tgttattcgg
     1621 gcgcagggtg tcctgcgcct ggtccaccat tattgcattg ctttataagc ggttaccgcc
     1681 tcagatacaa cctcgctgtt ggcatcaaga ccggaaatct gtgccagcgc cgcctgcgga
     1741 cctttgtcag cgatcagtgc tgccagttcc tgagcctgcg gatcatcttc actgcggaag
     1801 tgcattgcag cggcaatacc ttcaatcagg tttttatgtg gcagaccata ttccagcgta
     1861 ccgagcagtg gcttgatcag acggtcgcct gcgctcagtt tacgcagcgg ctgacggcct
     1921 acgcgctcta catcatcttt cagatacggg ttctcaaaac ggccgaggat tttctggatg
     1981 tacgccgcat gcttgtcagc gtcaaagccg taacgcttga tcagtaccgc gccactttct
     2041 tccatcgcac cttttaccac cgcgcggatt ttctcgtcga gaatagcgtc acgaatggtc
     2101 tgatgaccgg ccagttttcc gaggtacgcg gttatagcat gacccgtgtt cagggtgaag
     2161 agtttacgtt cgacaaatgc catcaggttg tcggttaact ccatgcctgg gatgttcggc
     2221 agtgcacctt tgaactgcgt tttatcgaca atccattcgc tgaaggtttc taccgtcact
     2281 tccagcggat cgttagttgc cgaagccgaa ggcggtacga tgcggtcaac ggcggaatcg
     2341 acaaagccaa cgtgttcttc tacccacgcg ttggcgtctt ccggcagggc gttcatcaca
     2401 tggcctttca gctgcgtggt accgcgtacc atgttttcac aggcgatgat gttcagcggg
     2461 gattcattac cttgttcttt acgtttcacc agccctttgg cgatagccgg agcaatacgt
     2521 tccagcacta ccgggccaac ggcggtagtg actaaatcaa cctgagcaat gagatcaacg
     2581 acatcatcac caatgctgct gacagcattg acgccggaaa cggtatctac ctgctcggtt
     2641 tcacctacca catgaacctg atagctatga cgggcattca gggcatcaag taccacctga
     2701 ttgacatcgg caaacgtcag ttgtataccc gcgtctgcca gcagtttacc gataaagcca
     2761 cgaccgatat tacctgcgcc aaaatgtaat gctttcatag tattaacctt catcaatgtt
     2821 tttacccgag agggctgggg tgaggctttt ccctcaccca aaccctctcc atgtggagag
     2881 gggccgcgcc ggatgcggcg taaacgcctt atccggccta caagaacgtg caaattcaac
     2941 aaattgtgat gcatcctgta ggcctgataa gcgtagcgca tcaggcaatt agccccacct
     3001 tctccatgtg gagagggtgg gattggatta cttatgacct gccagcagtt ccagcacttc
     3061 atccacgctg gtggtgtgtg ccagacgctc gatgacggac tcatcatcca gtgcattggt
     3121 caggctggtg ataacctgaa tgtgctcgtt gttacgggca gcaataccaa tcaccagacg
     3181 ggcaatgtca tcttcttctt caccgaagcg cacgccttcc gggtactggc agaacaccac
     3241 gcccgttttc agtacgcgat ctttcgcttc aaccgtaccg tgtggcaccg cgatagactc
     3301 acccagataa gtcggggtca gtttttcacg atccagcatc gcctgaacgt attccggctc
     3361 aacgtaaccg cctttcacca gctgctcgcc agcaaaacga atcgcttctt ctttggtggc
     3421 cgctttgcga ccgaggaaga tgttctccgc gcccagtttg aacaggttag cactggaatc
     3481 gtcaaagctg tctttcagac tgtctttcac tttcacttcg ttttcagtat ggcgctgggc
     3541 agcaaccaga cgttcggtca ggctggtgta caggccgctg tcgaggaagt tggtcagcga
     3601 aatatgctgt gcctgcggaa cctggcgcat agcgcgttcg gtcaggtcac ggtgagtgat
     3661 gacgaggtcc acatctggcg gcaggttgtt gatcgcactg ttagtgaccg aaatctgcga
     3721 cagacctgca tcctgaattt tcttacgcag cacgcctgcg cccatcgcac ttgaacccat
     3781 accggcgtca caggcaacga tgattttacg tacgtggctc aggtcgttag tcacatcgcc
     3841 cgcagacagc ggagatgcac ctttagactc agctttcatg tcctgcatac gacgagttgc
     3901 tgcttcaata tcatcttctt ctttcacttt gctggttttc agcaaaatag cagagacaac
     3961 aaaggagaca gccatcgccg cacacacacc cgcgatgtta gcgaaataag caccttttgg
     4021 tgtcatcgcc agtacagcaa ggatagaacc cggagatgcc ggagaaacca gaccaccgcc
     4081 caggatagtc agcgtgaaca cgccagtcat accgccgagg ataacggcca ggatcagacg
     4141 cggattcatc aacacatacg ggaagtaaat ttcgtggata ccacccagga agtggataat
     4201 tgccgcaccg cccgcagact gtttagcgct accacgacca aagaacatgt acgccagcag
     4261 cacgcccata cccggacctg ggttagcttc aatcaggaag aatatggatt tacccagttc
     4321 atgggactgc tgaataccca gcggcgagaa gataccgtgg ttaatggcgt tgttgaggaa
     4381 caggattttc gccggttcaa caaagataga cgccagcggc agcatgtcat ggacaaccat
     4441 gaagttaacg cccgcagcca gcattttgga cagggcttca acaatcgggc caatgccgag
     4501 aaatgccaga atggcgagga acatcccgat gatgcctgcg gagaagttat tcaccagcat
     4561 ctcaaaaccg gatttgatct taccgtctac ccagcggtcg aagtgcttaa tgcaccagcc
     4621 gcccagcgga cctgcaatca tagaaccgag gaacatcggc atgtctgcgc cgacgataac
     4681 gcccatggtg gtgatggcac cgactacgcc gccacgttcg ccgcctacca gcttaccacc
     4741 ggtataaccg atcagcagcg gcaggagata agtgatcatc ggcccgacca gcttcgccag
     4801 cgtctcgttc ggtaaccacc ctgttggaat aaataacgcg gtgatgatac cccacgcgat
     4861 aaacgcgccg atatttggca tcaccatgtt gctgaggaaa cgaccaaagc tttgcacttt
     4921 gatcttaata tcggatgaca taaaaacacc ccttcttatg tttgctgtcg cgcaggctgg
     4981 aagcccgagg ttttttgtta atgtggcggc agaggtagcc ggaccttgta gatactgcga
     5041 aatctggcac tgaatcggtt aactgtccag tcgacggcct tttgtgtgat atcaatcaca
     5101 taaatatagg gggtagagag gtaattgacg tgacgttcat cacaaaacgc ttgtgtaaaa
     5161 aagcaacaag gaggcgattt tcgctaaaaa tggcatttaa atgtgagtaa tgtcacattt
     5221 ttgttttgat ggtttgttga atctttgtga tctgaatcac aagatatttt cttacggcgt
     5281 tcacctgatg tgatctacag catgttatgc cttcgtcata caatcgttat gtaactaagc
     5341 aactcatcac ctctaaaaat tattctttgg tgttatggcg agaatcgcga acccggatta
     5401 ttgtcaaaat atcagcctga ctcccttctg gctgccagcc aaaaaacaac actgataaac
     5461 gtgaatccga ggcagatatg tttctaaact atttcgcgtt gggagtgctg atcttcgtct
     5521 ttctggtgat tttttatgga atcatcgcga tacatgacat cccttatctg attgccaaaa
     5581 agcgcaacca tccccatgcc gacgctattc atacggcggg ctgggtgagc ctgtttactc
     5641 tgcatgttat ctggccgttt ctgtggatct gggcgacgct ctatcaaccg gagcgtggct
     5701 ggggtatgca gtcacatgtt gcgtcgcagg agaaagcgac tgacccggaa atcgccgcac
     5761 tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag actgactatt
     5821 ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg tggcgctggc
     5881 gtgggcggtc tttaaaatct ttcgtattcc ggtgaatcag tggacgctgg cgacggcggc
     5941 gctgggaggc gtgtttctgg tgagtggttt gattttgttg atgaactaca accaccctta
     6001 cacttttacc gcgcaaaagg cagtgatagc gatccctatc acgccacagg tgacgggaat
     6061 tgttactgaa gtcactgaca agaataatca gcttattcaa aagggcgagg tgctttttaa
     6121 gctcgacccg gttcgttacc aggcgcgagt tgacagactt caggctgacc tgatgacggc
     6181 gacgcataat ataaaaacgc tgcgcgccca gctcactgaa gcgcaggcca acaccaccca
     6241 ggtttcagcg gagcgcgacc gtctgtttaa aaattatcaa cgttacctga aaggcagcca
     6301 ggcggcggtg aatccgttct cggaacgtga catcgacgat gcgcggcaaa atttcctcgc
     6361 gcaggatgcg ttagtgaaag gctcggtggc ggagcaggcg cagatccaga gtcagctcga
     6421 cagtatggtc aacggcgagc aatcgcagat tgtgagctta agagcgcaac ttactgaagc
     6481 aaaatataat cttgagcaga ctgtcattcg cgcaccaagc aatggctacg tcactcaggt
     6541 actgatccgc ccaggcacat acgcagctgc cttgccgttg cgtccggtga tggttttcat
     6601 ccccgagcaa aaacggcaaa ttgtcgccca atttcggcaa aactcgctgt tacgtctgaa
     6661 acctggcgat gatgcggaag tggtgtttaa cgcgctacct gggcaggtgt tccacggcaa
     6721 actgaccagt attttacctg tcgtgccagg cggttcttat caggcgcagg gggtattgca
     6781 atcattaacg gtcgtgcccg gcacggacgg tgtgctggga accattgaac tggaccctaa
     6841 cgatgatatc gatgccttac ccgacggcat ttacgcccag gtggcagttt actccgacca
     6901 tttcagccat gtttcggtga tgcgaaaagt gctgctaaga atgaccagct ggatgcatta
     6961 tctttatttg gatcattgag ggggaagggg gagattatgg aactttgctc cctgtaagga
     7021 ctaattattt ttgcatatta tttcatcggc tgtgatgatt tttcgttaat atccattatg
     7081 ttaagaagtt cgggatgagt gtgaactcat tggatttttt atccgtcttg atctgccgaa
     7141 ggatcagact atacattgtc gagatcgcaa tgatgatatg agttgttcct cctgcgacgg
     7201 ctctaatcaa aaaagagcag gagtgctttt ccatctattg cgatgatggc tttattatct
     7261 gaaggatgac agtcacattt aaaaaatatt taatctatag atcacaatgt agggtcatgg
     7321 aatatatgta tacaactatt ttcttcttca cttttaatta tcgtattcat tataaaacta
     7381 agtttaaaaa aactcctgga gagtgacttg ttaatatatt tttaaataaa attaagttat
     7441 tattcatcga tgcattttat agaatcatca atggccataa tagtactatt aactctccac
     7501 tttatatttt tatcatcact acaaatatta actgaattta tatcaatgat ttttttattc
     7561 ttatctttta ttttatttac tgtttcaatg caatcagc
//
LOCUS       DAZGCQ010000186         7501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000186 DAZGCQ010000000
VERSION     DAZGCQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.186"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..908)
                     /gene="glxR"
                     /locus_tag="ACHK07_004850"
     CDS             complement(30..908)
                     /gene="glxR"
                     /locus_tag="ACHK07_004850"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8105066.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1009..1785)
                     /gene="hyi"
                     /locus_tag="ACHK07_004851"
     CDS             complement(1009..1785)
                     /gene="hyi"
                     /locus_tag="ACHK07_004851"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO8105067.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1798..3579)
                     /gene="gcl"
                     /locus_tag="ACHK07_004852"
     CDS             complement(1798..3579)
                     /gene="gcl"
                     /locus_tag="ACHK07_004852"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO8105068.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3669..4484)
                     /gene="allR"
                     /locus_tag="ACHK07_004853"
     CDS             complement(3669..4484)
                     /gene="allR"
                     /locus_tag="ACHK07_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO8105069.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNSNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4562..5044)
                     /gene="allA"
                     /locus_tag="ACHK07_004854"
     CDS             complement(4562..5044)
                     /gene="allA"
                     /locus_tag="ACHK07_004854"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO8105070.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5274..6200
                     /gene="allS"
                     /locus_tag="ACHK07_004855"
     CDS             5274..6200
                     /gene="allS"
                     /locus_tag="ACHK07_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO8105071.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6269..7363
                     /gene="mnmH"
                     /locus_tag="ACHK07_004856"
     CDS             6269..7363
                     /gene="mnmH"
                     /locus_tag="ACHK07_004856"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO8105072.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
BASE COUNT         1897 a         1985 c         1824 g         1795 t
ORIGIN      
        1 tttgatatcg gccattttta ttgcgggtat caggccagtt tatggttagc cattaattcc
       61 agcgcctgca ctaacgcaga gtgatccaac tggctgccac cgtttgccgc gcaggtatta
      121 aataactcct ggcaggtcgc agtgtttggc aggttcagcg ccagtgcctt cgcactttgc
      181 agtgccaggt tgagatcttt ctggtgcagc gcaattttga aacctggatt aaaggtgcgt
      241 ttaatcatgc gctcaccgtg aacttccaga atacgtgagg aagcaaagcc gcccatcagc
      301 gcctggcgca cacgtaccgg gtctgcaccg gcttttgaag caaacagcag agcttcagag
      361 accgcttcaa tattgagcgc tacgataatc tgatttgcca ctttgcaggt ttgaccgtcg
      421 ccgttaccgc ccacaagggt gatattttta ccgagcagtt caaacagcgg tttaacacgt
      481 tcaaataccg cttcgtcacc gccaaccata attgacaacg tcccttcacg cgcgccgatt
      541 tcgccgccgg agactggcgc atcgagataa tcaccaccca gttcattcac ctgacgagcg
      601 aaacgtttag tttcaatcgg tgaaatggag ctcatatcaa caatggtttt gcccttcagc
      661 gaggctttgg tacaaccatt ttcaccaaac agaacttctt caacctgagg tgtgtccggc
      721 accataataa aaatgatgtc cgctgcttcc gttacctggc gcgcggtgtc gacattaaca
      781 gcaccgagtg acagtaattc atcagcaacc ggtccaatgg tcgtgacatg taattgatga
      841 ccggcacgcg ccagattaat ggccatcgga gtacccataa tgcctaagcc aataaatccc
      901 agtttcataa ttaacctctt tttaaatgtc gctattcctg aattcagaca acacgatccc
      961 ctgccaactt agcggcctaa aaaagcattg tctgaatagc gttacgtttt aacggtacgg
     1021 atccatccag cgtaaaccgg cttccgtggt ggtttggggt ttatattcac acccaaccca
     1081 accgttgtag tcagaatttt cgattacctt aaagagataa tcataattaa tttctccggt
     1141 ccccggttcg ccgcgatgcg gattatcagc aatttgcagg tgaccaattt tatcggccca
     1201 ctgagtcatg gtgttggtta attcaccttc catccgctgc atgtgataaa tgtcatactg
     1261 aattttcaaa ttgcagcaac caacatcatc aatcaatttc agcgcctgcc gagttccggt
     1321 gagatggaaa ccaggaatat caaaatggtt aataggttca atcagtaata aaatatcttc
     1381 tttcatcagc atattcgcgg cataacgcag gttttctaca agcgttgcgt gaatctgttc
     1441 gctgctgaaa ccagccggcg ttttaccgac cagacagtta atttttttat tacccagcgc
     1501 acgggcataa cgaatcgctg ctgctacgcc atcccgaaac tcttcttcac ggccaggaat
     1561 acaggcaata ccgcgctccc ccgccgccca gtcacccgcc ggtaaattgt gcagcgtatg
     1621 ttcgagttta ttactcgcca gcacctgttt taattcttca atgtcgtagt cataaggaaa
     1681 cataaattca acgccgcgaa aaccacactg cgcagctttc tcaaaacggg cgagaaaatc
     1741 atattcacca aataacatcg ataaattagc agagaaacgt aacattattt atctccctta
     1801 ttcatagtgc atgaagcagg tttcagtcgg tgcatccgct gcgttatcgg cgatatcttc
     1861 aaattccatg acgttatcca gttcgctgcc catcgaaata tttgtcacac gctcgagaat
     1921 aacttccacg actaccggta cccgatattg cgccattaag gctttcgcct gttcaaaggc
     1981 cggtgcaata tcttccggtt tgaagacgcg aatagcttta caacctaaac cttccgctac
     2041 ttttacgtgg tcgacgccgt aaccgttcac ttcgctggag ttgatattct cgaaagcgag
     2101 ttgcacgcag tagtccatgt caaaggcgcg ttgtgactga cgaatcaggc cgagataggc
     2161 gttattcacc agcacatgga tgtacggaat gttgaactgc gcgccaacgg ctaactcttc
     2221 aatcaggaac tggaagtcga agtcaccgga aatcgccacc acattgcgtt tcggatcagc
     2281 ggcacaaacc cccagcgccg ccggaatcgt ccagcctaac ggaccagcct gaccacagtt
     2341 gatccagtgg cggtctttaa agacatgcag catttgtgcc gcagcgattt gtgacaggcc
     2401 aatggtggtg acataacaaa catcgcgacc aaaggctttg ttcatctctt catacacgcg
     2461 ctgcggtttc accggcacgt tgtcgaaatg ggttttgcgc agcaaagtgc gtttgcgctg
     2521 ctggcagtcg gcgacccatt ctttacgaca cggcagacga cccgcttttt gcatctcctg
     2581 cgccacttca accagcagtg tcagcgccgc tttagcatca gagacaatgc cgagatccgg
     2641 acacagcacg cgaccaattt gcgtcggctc aatatcgata tgcacgattt tgcgcccttc
     2701 ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa cggttaccga taccaaacac
     2761 catgtcggac gccagcagcg ttgcgttacc gtaacgatgc gcggtttgca gacccaccat
     2821 cccggccatc agttcatgat cgtccgggat acagccccag cccatcagcg ttgggatcac
     2881 cggaacgctg gttagttcag caaactgttg taacagtgca gctgcgtcag cattgattac
     2941 cccgcccccg gcaacaatca ccggacgttc ggcctggatt aacatttcta cagctttttc
     3001 gatctgcata cggctggcag caggtttgta gaccggcagc ggttcgtaca tgtcaggatc
     3061 aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc accagtaccg gacccggacg
     3121 accagaacgc atcaggtgaa atgcctgttg cagcacgcga ggcaccagcg ccgcttcacg
     3181 tactgtaacc gccattttgc tgaccggttt agcaattgct tcaatatcaa cagcctgaaa
     3241 atcttcttta tgcagacggg cgcgcggagc ctggccggta atgcacagaa taggaatgga
     3301 atcagcagaa gcagaataga gcgcggtgat catgtccgtg cccgcaggac cggaagtccc
     3361 cagacatacg ccgatattcc ctgccgttgc gcgggtataa ccttccgcca tgtgcgaagc
     3421 accttccaca tgacgcgcca gaatgtgacg aataccgccg tgcttacgca tcgctgagta
     3481 gaacggattg attgcagctc ccggaacacc gaaggcggtg gtgatacctt ctttctccag
     3541 cacatacatt gccgcgtcaa cggctctcat ttttgccatt ttattcctac ctccattaat
     3601 tggaaaaatt ttccaacttt caaattttgt caaaaaaacc cgctccgcag agcgggatgc
     3661 gatagacatt atggatgtgc tttcagtccc aacgccgtgc tgatatcgcg ggcggtgtct
     3721 ctgaccagct caccctgact gacaaaacga tcttctgtca gtcttgatga aggcccggag
     3781 atggagatag cggcaacaac actacccaca tcatcgtaaa ttgctgaagc tatgcaattc
     3841 agacctacaa catgctcttc tttatctacg gtatagccca gttcacgcgc ttgttccagg
     3901 tccttcagca gggtgggcat atccacaagc gtagttggcg taaactgctg caaaccggtt
     3961 tgcagaatga tgctcatcaa ctcctcttcc gccagcggat aaagcagcgc tttgcccgca
     4021 ccggaagcat gcagtggcag acgactgccc agtggcgcac acatcctgac catcgattta
     4081 cactctaact gaccaattaa taccgcttca ttgctgttac ggatcgcgac attgaccgtt
     4141 tcgccagaaa gtaacatcag gcggcgcata aacggcccgg cgacggagag gacatcgcgg
     4201 ttatggatgt acgctgcacc gacgttaaag acacctaatc ctatatgcca ccagcctaac
     4261 tgactgtcct gatagacaaa atccgctgcc tgtaaaacct tcagcaagcg aaaggtcgtg
     4321 gagagcggca aatccagatt gagagaaata tcgctaaccg acgaacttcc cccacttttt
     4381 tccagatatt gcagaatcgc aattccccgc tctaatgcct gtgcgccctt ctgtgccaca
     4441 ggctccgcct gtcctggcct gccgcgccgt ctaacttccg tcatacctac gttaactccg
     4501 acctacaagt gagcataaac aatgaacaat tagtttatct tatgcaagtc ggttgcaggc
     4561 gtcacgcaaa acagagttcc tgttcaggaa tactttcaac atcacagttg tcactgccgc
     4621 cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc gaaaagtggg tgatgccaga
     4681 cgttacgatg gtaattcact ccctgttcgc cgttggtaat aaacgcccgc agggttgaca
     4741 ggtctggttt gtcgtcacct aacgccacga ccaccacaaa cacctcacct ttcatcggga
     4801 taaaggcctg agtacccagc ggatgacgct cgagttcgtg aatggtcagc ggcagattcg
     4861 ccggttgcgc gcggttaatg ctgataagcg tacggtcttg ctcaagaatc tcaaccagcg
     4921 ccaaatcgtg gtaacgctcc accaggccat tgttaatatg gaaaaaatcc cgttgctgcg
     4981 tttcgattac gtcgccataa gcactaaagg cttcctgact taacggtaat acctgaagtt
     5041 tcatcttttt gtccttgtaa cataattccg ttcaaccacc gccgaccggt cagcttggtt
     5101 tcaataacag gcaatctaaa accttaagag aacaacatca atacaaaagt tggaatattt
     5161 ttccaaaact agatttagtc cacaaattga acagggttat catgtaaaaa tactgataaa
     5221 tcagaggata agaaaatcac gcaacttgcg gatttaaaag gggatacctg aagatgttcg
     5281 atccagaaac cttgcggact ttcattgcgg ttgctgaaac aggaagtttt tcaaaagcgg
     5341 cagaacgatt atgtaaaacc acggcgacga tcagttatcg cattaaactt ctggaagaga
     5401 ataccggagt agcgctgttt ttccgtacga ctcgtagcgt aacgttgaca gcggctggcg
     5461 agcatctact ttcccaggcc agagactggc tgagctggct ggaaagtatg cccagcgagc
     5521 tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat tgtcatcaac aacctgctct
     5581 ataaccccca ggccgtcgcc cagttgctgg cgtggctgaa tgagcgttac ccctttaccc
     5641 agtttcacat ctcccgacaa atctatatgg gcgtctggga ctcgctattg tacgaaggtt
     5701 tttcgctggc tatcggcgtc acgggaactg aggcgctggc aaataccttt agtcttgatc
     5761 ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga tcatccgctg gcgaacgttg
     5821 aagagccgct aacagaagcg cagttgcggc gctttccggc ggtcaatatt gaagacagcg
     5881 cccgcacctt aaccaaacgc gtcgcctggc gattgccggg tcaaaaagag attattgttc
     5941 ccgatatgga aacgaaaatc gccgcccatc tggcgggcgt tggcattggt tttttgccaa
     6001 aatcgctttg ccagtcaatg atcgataatc aacaactggt cagccgggta atcccaacga
     6061 tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt tggcagcggc aaagcggtag
     6121 aagatattgt gaccttgttt acccagcgca ggccggaaat cagcggattt ttagaaattt
     6181 tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg ttggcgcgta aaatagcgcg
     6241 caagctgatt tgaggttcac ttttaactat gcaagagaga cacacggaac aggactatcg
     6301 tgccctgctg attgctgata cgcccattat tgatgttcgc gcccctatag agtttgagca
     6361 aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat aacgatgaac gcgccgccgt
     6421 tggcacctgc tataaacagc aaggctcaga cgcagcgctg gcgctgggac ataaactggt
     6481 ggcgggtgaa attcgtcagc agcgcatgga cgcctggcgg gcagcgtgcc tgcaaaatcc
     6541 gcaaggtatt ctctgctgcg cccgtggcgg tcagcgctca catattgtgc aacgctggtt
     6601 gcatgcagcg gggattgatt atccgctggt ggcaggcggt tataaggcac tgcgccagac
     6661 cgcgattcag gcgactattg aactggcaca aaaaccgata gtgctgattg gcggttgtac
     6721 cggcagcggt aaaacgctgt tagtgcagca acagccgaac ggtgttgatc tggaagggtt
     6781 ggcgcgtcat cgcggttcgg cgtttggtcg cacgttacaa ccacaactta gccaggcgag
     6841 ttttgaaaac ctgctggctg ccgaaatgct aaaaaccgac gcccgtcaga atttgcgcct
     6901 gtgggtgctg gaagacgaaa gccggatgat cggttcgaat cacctgccgg aatgcctgcg
     6961 cgagcgaatg actcaggcgg cgattgcggt ggtagaagat ccgtttgaga tccgtcttga
     7021 gcgcctgaac gaagagtatt tcttgcgtat gcatcatgat tttacccacg cgtacggcga
     7081 cgaacagggc tggcaggaat attgcgaata cctgcatcac ggactttcgg ctattaagcg
     7141 tcggctgggg ctacagcgat ataacgaact ggctgcaagg ctggatgcag cactgacaac
     7201 gcaactcacc accggcagca ccgacggtca tctggcctgg ctggtgccgt tacttgaaga
     7261 atattacgac ccgatgtatc gctatcagct tgagaaaaaa gcggaaaaag ttgtctttcg
     7321 cggtgagtgg gcggaagtgg cggaatgggt taaggcgcag tgatgaggga cgtatttgta
     7381 ttagtcgcga cttgatctga cacatggcct tgaaaggttg agagttaccg gttttgatat
     7441 gagtgtcgaa tccttatcca aaacaagagg taactctcat gcttcatact gccaatcccg
     7501 t
//
LOCUS       DAZGCQ010000187         7465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000187 DAZGCQ010000000
VERSION     DAZGCQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.187"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(884..2542)
                     /locus_tag="ACHK07_004857"
     CDS             complement(884..2542)
                     /locus_tag="ACHK07_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8105073.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGL
                     PYHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYT
                     EEWDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGF
                     IGLEVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVL
                     RTETGFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIP
                     DIYAVGDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVF
                     SLSIGSVGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQ
                     ASGKKGVDKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKG
                     DTVICHVRDVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKE
                     IIIGCQSGLRGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
     gene            2703..3050
                     /locus_tag="ACHK07_004858"
     CDS             2703..3050
                     /locus_tag="ACHK07_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8105074.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEA
                     NVTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLY
                     CPQADPDKVKKQP"
     gene            3597..3818
                     /locus_tag="ACHK07_004859"
     CDS             3597..3818
                     /locus_tag="ACHK07_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105075.1"
                     /translation="MTKLGENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTD
                     EHVPLYIRRLEYYRLLYRPKQTESYWKKR"
     gene            complement(3872..4405)
                     /locus_tag="ACHK07_004860"
     CDS             complement(3872..4405)
                     /locus_tag="ACHK07_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105076.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISHDFQRLEESVANMKVNADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLAEKPVTGKSRKN"
     gene            complement(4408..5334)
                     /locus_tag="ACHK07_004861"
     CDS             complement(4408..5334)
                     /locus_tag="ACHK07_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO8105077.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSG
                     NLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            5511..5909
                     /locus_tag="ACHK07_004862"
     CDS             5511..5909
                     /locus_tag="ACHK07_004862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105078.1"
                     /translation="MRISNIEWLKKRLGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKEIKYDLETSPNPDA"
     gene            complement(6103..7038)
                     /locus_tag="ACHK07_004863"
     CDS             complement(6103..7038)
                     /locus_tag="ACHK07_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO8105079.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNSPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSV
                     KALKYAAAIERSLCEKLDADANYSGLICKNPFHLEWQVKEWREEPYSLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLAVSTRTIRNWKSGK"
     gene            complement(7235..>7465)
                     /locus_tag="ACHK07_004864"
     CDS             complement(7235..>7465)
                     /locus_tag="ACHK07_004864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105080.1"
                     /translation="VLAKREALKQKIPVQNFPPRARKLVILTCSPFGTVEIDRASLSC
                     IVMQPSKNDSGAFFWFGLVFSCLFAFFAPESG"
BASE COUNT         2000 a         1957 c         1661 g         1847 t
ORIGIN      
        1 aaaaaaaacc gacctggggt cggggcaaga atcagagtct gtgagaaatt ttttttagtt
       61 gtggaatgcg tcaatgttcg caaccgcccc aaaataccct gaggctgata aaaaacaact
      121 ggcactatcc cgttccggat aaaaaaacca caaaagtggg gaatccaaga gaaacgatag
      181 gtgtcaaatt gagtcagaat attgacaatg aaatagttac gcaaataaat ttagctgcat
      241 cacttattct gattcaatac tcaataaaaa atgcccgcat aataaaccac atttacaaag
      301 atatatatca aacgaaacaa ccaagttcac agctcaggat aagaattttt ctctatgtaa
      361 tagataaaac acagtttttt tataaactgt aatatatgag ctgtaatatt catgtattaa
      421 ttgttttttt gaaaagcatc acaaatttat tttttacgca tactgcccta ttgcaaccag
      481 caatggtttt cacttatttt caacatctca tttaatacga gttatacttc aacccgttta
      541 aagtgcaaca ctaactgagt atcatttgtt ttccttaccc acccctggat tcccaggggt
      601 tttttataac tacaaaccag cacaacaaaa aaacaaaaca caataaaaac aatgaattat
      661 ataaaactca acgatatctt tgtacggaga tttacattca ggaacattgg ggtcagtttg
      721 gatatagaga attattgtac ggtaagcctg ttttttgaac agtccacatt atcgcaatag
      781 ggtaccatcc agggcgttaa ttatttttgt agatttctat tccatataat acgttttcaa
      841 tgaatctgga agatgaggtg ccatgtgtat ggcacctcat ctcttattga ctcattgatt
      901 ccgtaaccgc ctgccaagtt ataaagccac cactcagatt atatgtccgg aagccgtgag
      961 actgaagcat acggtatgcc acatgccccc gcagaccact ctgacacccg ataataatct
     1021 ctttatcttc aggcaactta tcaagattct cccgcaggga atcaagaggg atggatagcg
     1081 cgtcaggata ttcaccaaat tgtttcagtt ccgcaggaga acgaatatca agtaaacaat
     1141 aactgcccgg ttcccgttgt aacacatcgc ggacatgaca gatgaccgta tcgcccttga
     1201 taacatttgc cgccaccatt ccagcctggt taacgacgtc ccttgcactg ttgaaaggcg
     1261 gagcataggt cagctccaga tgctcgagat cgttaacggt aagccccgcg cgctgtacga
     1321 cagccaggac atcgatgcgt ttatcaaccc cttttttccc tgacgcctgg gccccgagaa
     1381 ttttaccggt atcggggctg aacagcagtt tcaggctgat cattgttgcc cccgggtagt
     1441 aactggcatg atcagcggca tggacgtaaa ccttctcgta tcgggtacca tgggctttca
     1501 gctgcttttc attcgccccg acactgccaa tactcagcga aaatacctta caaatggatg
     1561 tcccctgact gccatggtaa aggctgtgac gatcgagcat attatcagca gcaatgcgtc
     1621 cctggcggtt ggctggccct gccagaggaa cattagcggg ttcctgaaag acaaggtcag
     1681 gtgtttcaac tgcatccccc acggcataga tgtcagggat acttgtctgc atacaggcat
     1741 tgacacttat gcctccccgt ttacccacgg cgagcccggc tcctgtggca aggctgctct
     1801 caggttttac cccgatggcg agaataacca tgtcggtctg cagaaaccca ccgtcagaca
     1861 gtgcaacgcg gaatcccgtc tctgtccgca gtacctcggt gagcgccgtt ctgagacgca
     1921 gatccacgcc atgactcctg atttcctgat gcagggccga tgccatttca ggatcaaccg
     1981 gagccataac ctgttccccc atctccagca gggtcacact gatccccctt tcgctcagtg
     2041 cctccatcac ttcgagcccg ataaatcccc ccccgacaag cgtggtgtga gcgatattat
     2101 gttgctcaat ccacgcctgg atagcatcca tgtcagtaag atttcgcagt gtaaagacac
     2161 ccgcttcctg tagtccgggc aaggggggaa caaccggagc ggcaccggta ctgaggagca
     2221 gacgatccca ttcttcggta tagacctccc cggaggtcag atttttaacc gtcaccatct
     2281 tattaaccgg gtctactgac atcacttcat gacggatacg gacgtctatg ttaaaacggg
     2341 atttaaaatc ctccggggtt ttcagaatca gcgactgccg taacggaatt tttccgccta
     2401 tatgataagg caatccacag ttggcgaaag agacatcgct tccccgctca aacacaacaa
     2461 tactgacatc ctctgataac cgacgagccc tggctgcagc ggatgctccc ccggcaacac
     2521 caccaacaat cactattttc attacatcct ctctgttcac tcggggcaaa atatctgcca
     2581 tatataatta gttacccggt ccagctggac gctgcagacc tgaagatcac ggtggcaaaa
     2641 ttacgccact atcattatac tgaacaatat tatataaaaa tacataatat aagaaaccag
     2701 caatgcccat gaatgaagac acaaaacgtc agactgatat gaaaaatgca gccctggggg
     2761 cagcagatgt cctacgggga ctggccaaca gcgaccggct attgttgatg tgtcagttaa
     2821 gccaggggga agcgaatgtg accgagctgg aagcggcggt cggtatcact cagccgaccc
     2881 tgtctcagca gttgggtgtc atgcgtcgcc tgaagctggt cgagacccga cgggcaggta
     2941 aaatggtata ttaccgtatc ggggataacc gcatcatgac gttgttgaat accttatatg
     3001 atctctactg cccgcaggcg gaccccgaca aggtcaaaaa acaaccttaa cgtacaataa
     3061 ttttcgatat ccaaactgcc cccaacattc accagcacag ttagacatta gcaatgcaaa
     3121 atcaatgcga ttccggtgcc gtatgtcagg gagaattatg taaacggggg gggccggaat
     3181 cgcattccag ctcatatcat taaatacaga aaaaaacctc tgagagcaca gaggggataa
     3241 ggaatatgaa tactccggtc aggatattat cctggctttt atatacaaca tgctcacaag
     3301 gacaacaact agctagttag ttaatagata ttaaaaattt tagagctgaa accttaaagc
     3361 ttgcttccat tccatgtttt attataaacc ttatcttgaa aactgaaagg aagtacggca
     3421 gattaattaa gttgcgtgca ttcttttcac gttgtaatac aaacaagaac acctgcgaag
     3481 ttatttctcc ttacatatcc ccctatgcca acgggggatt ttttctggtt cactaacgca
     3541 taaataatag tgacatacta taattcacga accgtgataa tatcagatca atcatcatga
     3601 caaaattagg tgaaaatatc ccacatatta tcgatgcagc tgtagatttt atggcatcaa
     3661 gccaggcatt tcgtgagtat ctgaaaaaac ttccccctga taacacaata ccgccagatc
     3721 ttactgatga gcacgtaccg ttatatatac gaaggctgga atactatcga ctgctttatc
     3781 gaccaaagca gacagaatca tattggaaaa aacggtgaac ggttcgcgaa atcctttcgc
     3841 gctccgtgag gcgttggaca aacgaagcgc gtcagttttt ccggctctta cccgtgacag
     3901 gtttctccgc cagtttatct tccagtgtca caactttctg atacaccgcc gctgtggcgt
     3961 tgtagttcct gtcgagtatt gttttcattc cccatccgag cacacagaac ataaccagaa
     4021 aagccactga caccccaccc agccatttcc agctataccc tttaagggtg ttgtattcct
     4081 gcacgatatt gctgtgaatt gtcgcaatat ccctgttcag ggcatccgcg tttaccttca
     4141 tgtttgcgac tgactcttcc agccgctgaa aatcatggct gatgcgctca cctatcagcc
     4201 ctgctacgtc cagcttctgc acctcgtttc tgacttcatc cctgacctgt tttgccacac
     4261 cgtcagaaat atcccgatat ccgcgaagtg tttcccttgc cacagccaga gaatcagcaa
     4321 cagctttctg ttgctccatt gcggtatgtt gcagatgctc caccgataac aggagcgtga
     4381 gtaatttttc ttctgtgctg gccatgttca gaatcccata gaaaatccac gtgacggtga
     4441 tttaacccgt tcaggttcag ttcggtcctg tctgctcact ctgtctctca tgtcaaagcc
     4501 atgctcccgc gcaacatcat gttgaagcct tctgaccagt tcatgttgct taaccggagt
     4561 gtccagccat tccctgatgt catgcccgac cgttcgcgcc tgttcaagat ggcgggaata
     4621 gtccggttca gaccgttttt gcatgatgtc atgacgctgg gcatcatcaa gccgtccagc
     4681 agcttcaaaa cccagatgca cttcgggacg tcgttcaatg ccctgttctt tcagcgtccg
     4741 gcagtcaatg aagccccttt caaagccatg ctttctcata tgttcgttgt gcttcacctc
     4801 ccagcgtttt cgcaattccc gcaactgctc tttttgctga gtgggggtca ggtttccgga
     4861 atcctttttc gcacctcccc ggtcgggata cctggcgtta tagcgccgga aatactgttc
     4921 cggtgtacgt tcaatgccat cattcacacg ctgactcatc atgatatgcg catgaggctg
     4981 ttcgccgccg tcaatgctcg ctttcggatt gtgaatggca aaactccagg cgtgccgttc
     5041 gccagcttcc tgacgtacaa aatcctgcac cagttcaagg cgctgttccg gtgtcagttc
     5101 acggggtaat gcaatttcaa gttccctata ggtcgaaccg ttcgcacgtt caaactggtc
     5161 agccgcctgc cagaaatggg aaggattatc cctggcccat tccggcatgt ttccgtgtgc
     5221 ggaatattcc aaatcctgac ggtcgcggta tttctctttg cgctcgatgt aatcggcgtg
     5281 gtttgcagcc tgccccttgc caccaaattt aacgctgagg tgataggttg ccacggtttc
     5341 agccctctgc aggagcgcac tgtttctcga cgaaggagag aaacatataa gtgctcttcc
     5401 ttctaatttt acgaattagc acggaagata ccccctgcac ccgccgaccg caagcgatat
     5461 actgctcgac cacaacacac cgcaaaaaaa caccagggag caccacgaac atgcgcatca
     5521 gcaacatcga atggctgaag aaacggctcg gattcatcag gaagctcgga gaacagaccg
     5581 cgcgacagag acagattatt gacctgctcg acaacgaagc cggactcaca gaacaggaac
     5641 gcaaacttct ccacgtgctg gcaacagcgg aaaaaaacga cttacaggca caggagaacg
     5701 agcgaaaaca gacgatccag aaacgaatcg agggaaaaaa acaacgacgg gaacgcaatc
     5761 accggctctt tctcgcggct gggctactga tcgaagcagg actggtagac acaaaaacag
     5821 gcgagctatg ctataaaaaa gacaggatat tacaggcact taaggaaatt aagtacgatc
     5881 tggagacatc cccaaaccca gacgcgtaag tgcctccatc cgtgttccgg ttccctggtg
     5941 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagca ctaccagcac
     6001 aatcatgcgg aaagcagcac cattgcaggg ctgaacgggg aaaaacggca aaaaacctac
     6061 tgctcaaaaa aacgacagaa aggcgctgtt atctgatata ggctattttc cgcttttcca
     6121 gtttcgaatg gttcgagtag aaaccgccaa catcgccgca atagctttct gagaatatcc
     6181 attctccagc atacatagcg ccatgaaacg cttgtcatcg taggcttcgc ctttagcgat
     6241 gccacctttc ctgcctctct tggcctgtat ttctggcgtg tgcgtatcag ccacatactg
     6301 tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatactct tgccaatagc
     6361 ccgacattca ggcggtgaaa gcggaacggg aagcgatgcg ttgtacattt caacacgctg
     6421 aatcacggca tcaagccact gtgagaatgc tggccagccc tgccgaatcg ccctgtaagc
     6481 ccatttgcgc gtcatttcga acagatggca gtttctcccc attccgtaat gtttatcgat
     6541 gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga gggagtaggg
     6601 ttcctcgcgc cactccttca cctgccattc caggtggaac ggatttttgc aaatcagccc
     6661 gctgtagttc gcgtctgcgt caagtttctc acacaacgag cgttcaattg cggcagcgta
     6721 tttcagtgcc ttaaccgaac tgtccggtgc ggttctcacg gcgatattga gtgcgtaaag
     6781 caggtgggcg tgcccgttca caggattttt aacggtgacg ttcggtgcgg gtgagttccg
     6841 gtcgctccag tcgatcgctg cccccacgcg gtcaacgtca aaaaccagcc agaactgcgc
     6901 atgaggctgg ttctgctgga tgtaccgagc aagaagcgca cgccctttgc cggagatgcg
     6961 aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt cgtcgaagta
     7021 ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac ctcgcccctg
     7081 tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt ttttcagtcg
     7141 cttacactac ccccaacgac aaaacctgtc gagccttccc gcaaaaccga tccatttacc
     7201 ataactcccg ttgtacgtac cccttaaaac gccctcagcc gctttctggc gcaaaaaacg
     7261 caaaaagaca agaaaacacc aggccaaacc agaaaaacgc gccagaatcg tttttagagg
     7321 gctgcataac tatgcacgaa agcgatgcgc gatcgatttc gactgttccg aagggcgaac
     7381 aggtcaaaat gacaagtttt ctcgccctgg gtggaaagtt ctgcacgggg attttctgtt
     7441 tcagagcttc acgtttcgcc aggac
//
LOCUS       DAZGCQ010000188         7442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000188 DAZGCQ010000000
VERSION     DAZGCQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.188"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..618)
                     /locus_tag="ACHK07_004865"
     CDS             complement(37..618)
                     /locus_tag="ACHK07_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8105081.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            775..3813
                     /locus_tag="ACHK07_004866"
     CDS             775..3813
                     /locus_tag="ACHK07_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO8105082.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            complement(3835..4149)
                     /locus_tag="ACHK07_004867"
     CDS             complement(3835..4149)
                     /locus_tag="ACHK07_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO8105083.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLGVLRPFPPKVSL
                     LA"
     gene            complement(4332..4613)
                     /locus_tag="ACHK07_004868"
     CDS             complement(4332..4613)
                     /locus_tag="ACHK07_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8105084.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            complement(4594..4923)
                     /locus_tag="ACHK07_004869"
     CDS             complement(4594..4923)
                     /locus_tag="ACHK07_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8105085.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            complement(4965..5158)
                     /locus_tag="ACHK07_004870"
                     /pseudo
     CDS             complement(4965..5158)
                     /locus_tag="ACHK07_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5858..6715)
                     /gene="repA"
                     /locus_tag="ACHK07_004871"
     CDS             complement(5858..6715)
                     /gene="repA"
                     /locus_tag="ACHK07_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO8105086.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            complement(6708..6782)
                     /gene="tap"
                     /locus_tag="ACHK07_004872"
     CDS             complement(6708..6782)
                     /gene="tap"
                     /locus_tag="ACHK07_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO8105087.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(7006..7266)
                     /locus_tag="ACHK07_004873"
     CDS             complement(7006..7266)
                     /locus_tag="ACHK07_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO8105088.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
BASE COUNT         2141 a         1568 c         1619 g         2114 t
ORIGIN      
        1 ctaaaagtct caccttgctt aggtaattaa ttgaatttat ttataaaacg ttgcaatttt
       61 agcatcattt tttattgttc tttttaacct gaagtacgtc gatcgagaga tacctgtttt
      121 ttctgtcaca ctgcccagag attctccctg tgatataagc tccaacgctg taggcgttcg
      181 tggatgcttc ttcctgccaa attttacgcc tgcggcaagt gccgctattc ttccgtcgtt
      241 tgtccgctcc agaatacgct cacgttccgc ttcagcgaca gcagcaagga tttggataac
      301 cattttgccc attgtccctt ctgtactgag accgttctcc aggaaccgga tggcgatccc
      361 ctttttgtaa cagctatcaa ccacacgaat catgtcagca gtgttacgcc ccagtcggtc
      421 catttttgta cagatgatta cgtcgtcttt ctctgcacgg gccaacaacc gctgtagtcc
      481 ttcccgttca tcagttgcgc cagtcatcat atccgtaaaa atacgatctt cccgtacccc
      541 ggcatttttt agctccgaga tttgatgtgt tagcttctga tgactggttg atacccgtgc
      601 ataccccaat aacgccattt ttagtctcac ttatcgtttt tgacactgca tggtatcata
      661 atgtacaaaa tacgattttt gaaacctgct tttatgctgt tttagacagg tatcagatat
      721 cttaatattt gatactagcc atatttaaca aatatttagc attttggtaa atgtatgcag
      781 aagtacaaga aaagaattag tatattaaca aaaagtgaaa taaatgaatt atatcaagtc
      841 ccctctttta atcctgttga aagggatgag tttttttcgc tggatatcag ccttaaaaga
      901 gaaattgata aaataataaa tatagaaagc agggtgtatc tgatattaat aacagggtat
      961 ttcagatata aacctgtgat tcctgaattt acattaaaag acgtaaaaag ggatgtgaaa
     1021 tatattatcc agacatatta taaaaacgaa agtactaatc tcaatttaaa catacctaaa
     1081 agtacaaagt accgccttgt taaacgaatg ctttctactc taggatttga acaactcacc
     1141 ccatctataa aaaatgagtt aagtgaacgt ctgaatgatg ttgctactat atgtatggaa
     1201 ccaaaatata tttttgatga attacttgct tttttaggac agcggcgtat agctcttcct
     1261 ggatacagta ctgtccagaa cctcgtttca gatgctttaa actttgaaag gaaaaggaca
     1321 attaatattt tgtcaaagag aatttatcct gccactgcac aaaagattac atccatcatc
     1381 catgatgatg gaatattaaa ttccatcagg ggatataatt atagcacgag agacttctca
     1441 gcatcagaaa tagaaaatga attaagaact cacaatgtga taagtagtat ttactatgaa
     1501 ataaaagata taattaattc attaggtata tcccagggaa acatggagta ctatgcaact
     1561 atcgtaaaac acagtacaac attcagactt aagcgacacc ctaaatggca aggaatactt
     1621 tatttgtgct gctacttgta cttcagatac agagagataa atgataaaat aatcacagcc
     1681 ttcagatatt tagtaaaaaa acataaagac gcttcagttt tgtttgcaaa agctcgggca
     1741 acagaggaaa tagatctaat aaataaaaag cttaagtttg cgagtaaaat atttaacttc
     1801 ttcgttgata gtaacattga agacagcatc ccttttggat atgttcgtga aaaggttttt
     1861 tcacttatat cgaaagatga tatgcatctt gtgagtgaat tttttgacaa aaataaaatc
     1921 gatgtaattg aatatcaatg gcagtatatt gacagccatc accttaagat cgccaattca
     1981 atccgaaagc tttttctggc tatagatata gtatatgaca acgagaagtc tgttattggc
     2041 cagcaaatta ctgctgccaa ggatgatctc agaggaaaag gaaagctgga tgctaaggat
     2101 ctgagggtaa ttcatccatc cgagaaaaat tacattgtaa aagaaggaga aattaacact
     2161 caacgattcg aatttcatat gtacagcagg gtatctaacc atctggaaaa cggtgtcttt
     2221 tatgctgcgg agagtgaaca aaacaagaga ctggaagatg atctcataga acgaagttac
     2281 tggcataaga ataaatcgat tttgatagag aaaacaggcc ttgaacgact tatcacacca
     2341 ataacgcaga ctcttaatga gctggagaat acctttagcg ataaactggc tcgtataacc
     2401 attggaataa actcagatgc taatgagttt gtgaaaaaac aaccacactc caacagatta
     2461 cagtggagtc tggccagtaa aaaatggaag accagcatcg ataatccgat ttacaatcag
     2521 ataaaaaata tcggaatagt tgacgtaatg aagtatgtaa acaatgagac gaattttttg
     2581 tctgtgttta atgccatatc atccaggaaa aataatcttg aagctgacta taatgacctt
     2641 cttgcctgta tatttggtaa tggcgcaaat tatggtgttc acagaatggc atctgcatca
     2701 gaccgtagta ttggaatact acgtgatgtg aatgacaaat tcattcgtcc agaaacaaca
     2761 ggaatggcca acacagtaat atctgatgca attgcaaatc tttctgtata taaatactgg
     2821 acgataaatg aagattctcc atttggaagt gttgatgggc agaagcacac ttgcagaata
     2881 aatacattta aagcccgtta ttctgccaag tatttcagaa aggggaaagg ggtttctgcg
     2941 atgacactgg tatctaatca tgtgccatta tcaactgtgg taatttctcc aaatgaatat
     3001 gaaggtcatt ttgcatttga cttgttatat aacaacacca gtgaaataca accaaagtca
     3061 ttagcgactg acaatcatgg tataaacaac gttaattttg cgattcttga catttttggc
     3121 tatcagtttt caccacgtta tgcaaaattc aagaaaatat ttaatgaatt atttgatgtt
     3181 atactggaga gtgataaact cattataaaa cttaaaaaac aatttaactt taagctcatt
     3241 gaacatgaat gggaaaacat ccaacatatt atttgttctt taagcagaaa aacaaccatt
     3301 caaagtacgg ttgttaagaa gttatctaat acaaagcgta gcaacaagac attagcagca
     3361 cttcgggagt atgaccgagt tataaaatgc atatatgtcc tggattatgc tgacaataaa
     3421 accttaaggc aatttgtgca acaagcactg aacagaggtg aagcttatca tcaactaaga
     3481 agagcaatag catcagtaaa tggaaatcaa tttcgcggtg gtaatgacta ccaaatagat
     3541 caatggaacg actgtgcgag gttaatagct aattgtgtaa tctattataa ttcagcaata
     3601 ttatccaggc tggtcgagaa atttgaaaaa gaaaataata aaaaagcgat agatgttctg
     3661 gctaacttat ccccagtagc atggagacac attctccttg gagggaatta ttcttttgaa
     3721 gatcaagttg cgatcacaaa tttggacagt cttttggagg gagtagaccc actaagtgat
     3781 atcggagata ttgagtccga atatgacggt taacaacatg ataaataatg gtttttatgc
     3841 taagagtgag acttttggag gaaatgggcg cagaacccct aacggattgt cagtactgag
     3901 gctgtctgcc caggcctgta cagcctgatg gtcgataatc tgtccggata tcacatcgtc
     3961 cagggcttcc agcgtcaggc gattacgctc ctcctcctgg gcaagccatg cagaaagtgc
     4021 ctgtttgata acccagcccc gggagcgttc cagtctggcg gccatctggt cgactttatc
     4081 agccataggt aatggcacat gcgcggtaat tacttttgtt gcctgtgcgg tattgttttt
     4141 catttgtata tccgattagt tgttaatcat atttaatcat agtgattaca ttcaggtggc
     4201 acaaggcaaa cgcagcccac agaagccgaa ttactttcag agaaatcgac acatcaggca
     4261 gatttaccat aacgtagcta attaaaattt agttactgat ttctccagaa ctggcagcca
     4321 tagagcagag ttcagtacag cgtaataaca ggttttttga taccgcacgc atgggcatag
     4381 cgagtcaggg tgtcgatgct ggctttagtg atgttgtttt ccaggcggga aataacagga
     4441 ggagtaaccc ccatgcgttc agcaacctgc gcttttgtca aattgtcatg cttacgccat
     4501 tctgcaagca actccctgag tttctcctta cgttcttctt cctgccatgc agcgcgatat
     4561 tcgtcgtcct gcatccactc gttatgcagt tctttatggc ttaccagttt cattaagtac
     4621 ctccgctaac cgctttttgg cgagatcgat ttcttttgct ggcgttttct gagacttctt
     4681 gatgaaaacc cggagcatga tgatggtatt gcctttgtgg tatacccaga tgcctctggc
     4741 tatgtccgtc cccatcgtgc ggatctcaaa aagtccatcc cccaggggct ttgtatccgg
     4801 ttcccgcagt aatcgggcgt catattccag tttgctgatg agacggtcaa acttcaccct
     4861 gatttttatg ggtaatgctg tggcttcttc cctggcttcc gggtgataaa caacgctaaa
     4921 catatgacct cccatttcga taaacattaa cctatatgct aatcttatgc aaggtgaatt
     4981 aacctgtaag ctaatgtagc aacttgttgt ttatgcgcgt cagcgcatgt aaggcggcac
     5041 gctgtggtta catgtgatac cggagtaaaa ccgccgaagc ccggcgtaag ccggtactga
     5101 ttgatagatt tcaccttacc catccccacc ctgcaaagcc atacccgctg tcagctatga
     5161 tagagcttat gtgcgcggtc ggagtggtcc cgacgagggt ttacccgaag tcggggcgta
     5221 tctccgcgtt agcgggccgt cagggccgct tacgagcgtg tactgaggac ttccagccag
     5281 aagactgaca gcgatgataa tgtagttaca acattcataa ttaaaagcga ctctgttccg
     5341 gccctttggg ccggggcggg gccgctttta agttatgagg gaggggcttt gcggtttcgg
     5401 ttctgcgctg gaccggggtt ttcccggtgc ttgtatttga gtgttgtaac taaaatggct
     5461 ccggtcgggg cccgccgctt gcggtgggag gtgcatatct gtctgtccac aggacaggca
     5521 atgaataggt tttcttttta aatgaatgta attaagtagt ttaaaggaga tataaacagg
     5581 tgtttaaaag atacattgca ccctgtaacg ctgacggctg gcgctttatg acatgaacgg
     5641 ttgtaacctt atggggaagt cccttgcagt taaatgtgga taagcaaaat tccacgtctg
     5701 tgaggcatgt tttgtatcga aaacaggggg gccggatgca ccagaagcgg gatgatgaga
     5761 ttgttttttg tatgtggtgc tgtttttttg ttcgctgaca ggcttcaggc gtgcagatgc
     5821 ctccggcgca ggccggatta ttcagatgag gtaaaactca gggggaggca gtggccagcc
     5881 gtgtgtagtt gttgttccgc gacattatca tgcgttcctt cacacgacgg tcgatttcgc
     5941 gcttcacggc ctccaggctt ccccggaaac gcccttccgc gatttcacgc gtcagctgcc
     6001 gtttcaccag ggtgacaatg tcctggcggg ttctgcctgc atcgcggcgc gcacgggcac
     6061 gtttcatgcc gtgggactta agctctgtct ggtaactgcg gaaacgctca cggacaaaac
     6121 gccaggcttt cgctatcagc tcatccatac ccagggtatc cagcccctgc tttttgcgct
     6181 gtctgttttc ccataccaca cggctgcggc gcgcagctgc cactgcatcc tcagacacat
     6241 caagggcggc aaacagcgcc ggtgtgaacg tgatatcggt cggaatgtag cacccgataa
     6301 gcgggtcata ttccgtctgg taggtaatca gtcccagctc tgacaggaac gtcagggccc
     6361 gggtggcccg ggtgatggag agttttcctg caccggactc tgtcgccagt ccgcactcaa
     6421 tggccagtgt ggtaatggaa cactggacgc ggttggccag cgggtcataa tggaaacaca
     6481 gcccctgcag cagcgcatca atagcccgtc gacgcagcac cggtggcatg cgtcgacgca
     6541 gaccacgcga acgggcatgc gccacatgaa tggcgaaatc aaaacgggag gtgaagccca
     6601 ccgccttttc catcagtttt tcgcagaact tcagcgttcc ggcaccttta cggggtgtaa
     6661 acaccggatt cgggttcttt acctggcggt aatacgtttg ttgaagatca gtcacaccat
     6721 cctgcactta caatgcgcag aaggagcgag cacagaaaga agtcttgaac ttttccgggc
     6781 atataactat actccccgca tagctgaatt gttggctata cggtttttag atggccccgg
     6841 taatcttctc agtcgccaaa cttgtcgaag attatcgggg ttttcacttt tctggctcct
     6901 gtaaatccac atcagaacca gttccctgcc accttacggc gtggccagcc acaaaattcc
     6961 ttaaacgatc agtaatctag catgctacgc cacaaagtaa agtctttact ttagtatatc
     7021 cagtctctgc agttcatctt tgatgatttt ctcaacgaac tgagcctgtg ttatcccctc
     7081 tctctcgcag tactcaacca tgagatcttt cagaggattt ttgacaaaaa cttttatctc
     7141 tttgtgtgta agacgttttc ttgcaacagc ggccatttgt ttctcagagt cagtcatagg
     7201 cttacctctg cgcacaaacc gcttttgact caatgaggaa gtcactgcat tttctgtctg
     7261 cgacatctcg cctcctcaat tctcaaacag ggatcgtttc gcagaggata ctacagtttt
     7321 ttgaaatcag cgacttgaga attgtgacga agatctgtgt tggcgcacaa atcaacggtg
     7381 attactgttg tttaatgtga tttttgttgc gaaatggctg aaaaacagga aatgccttaa
     7441 cc
//
LOCUS       DAZGCQ010000189         7419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000189 DAZGCQ010000000
VERSION     DAZGCQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.189"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHK07_004874"
     CDS             complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHK07_004874"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO8105089.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHLSNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1385..2389
                     /gene="argC"
                     /locus_tag="ACHK07_004875"
     CDS             1385..2389
                     /gene="argC"
                     /locus_tag="ACHK07_004875"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO8105090.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAQTQSLI
                     "
     gene            2397..3173
                     /gene="argB"
                     /locus_tag="ACHK07_004876"
     CDS             2397..3173
                     /gene="argB"
                     /locus_tag="ACHK07_004876"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO8105091.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3234..4607
                     /gene="argH"
                     /locus_tag="ACHK07_004877"
     CDS             3234..4607
                     /gene="argH"
                     /locus_tag="ACHK07_004877"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO8105092.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHK07_004878"
     CDS             4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHK07_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO8105093.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHK07_004879"
     CDS             complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHK07_004879"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO8105094.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1715 a         1828 c         2096 g         1780 t
ORIGIN      
        1 atcgcatccg acattattgc ggcactggag tttggcaaca gtgccggatg cggcgcaagc
       61 gccttattcg gcctacgttt taatgccagc aaaaatggtg aatcacctgg gtgatcagtt
      121 cgcgggtggg cttgataaac ctcgtctcca gatattcatc aggctgatgg gcttgattga
      181 ttgaacccgg ccccagaacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac
      241 agtagttcac cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat
      301 gatttggcgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac
      361 ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga
      421 gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt
      481 tagacgcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg
      541 cttcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt
      601 cgtgcattag ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactgac
      661 cctgaatacg gatggcgtta gagagatgac ctttatgtgc gcgtaccggt tgtagtgacg
      721 tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg
      781 ctccggccat actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt
      841 tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg
      901 cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc
      961 agcgaccgtc atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc
     1021 cctgtccggt acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt
     1081 ccacattgaa gcctaaattt ttaaaccagt ccgccagcag agtgattaaa tctgcattgc
     1141 tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa
     1201 tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat
     1261 caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac
     1321 cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc
     1381 cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac
     1441 ctatgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa
     1501 tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc
     1561 gttgcagccg atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc
     1621 caccgcccat gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt
     1681 gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg
     1741 ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggag
     1801 cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcggc
     1861 acagctggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt
     1921 gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt
     1981 ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc
     2041 cacacacctc ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cacgcggcat
     2101 tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tcgcgcaagt
     2161 gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc
     2221 gctgaaaaat gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca
     2281 tttgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca
     2341 gtgcgccaat attcgtttcg gctatgccca aacgcagtct cttatttaag ggtgcaatga
     2401 tgaatccatt aattatcaaa ctgggtggcg tactgctgga tagtgaagag gcgctggagc
     2461 gtctgtttag cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtcc
     2521 acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga
     2581 aaaatggcct gcgggtgacg cctgcggatc agatagacat tatcactgga gcactggcgg
     2641 gaacggcaaa taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt
     2701 tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggccatg
     2761 ttggactggc gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggttatc
     2821 tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg
     2881 accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg
     2941 tcagtggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag
     3001 aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc
     3061 tggatgctgc gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc
     3121 agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt
     3181 tggccggagg cgcagttttc cggcattgaa tttcaaaata aggaaacaga gttatggcac
     3241 tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac
     3301 tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag
     3361 ccctggtcac ggtaggcgtg ttaaccgcag aagagcaggc tcaactggaa gaggcgctga
     3421 acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag
     3481 atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc
     3541 tgcataccgg gcgtagtcgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag
     3601 ataccgttag tgagttactg acggctaacc ggcagctgca atcggcgctg gtggaaaccg
     3661 cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg
     3721 tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt
     3781 tgcaggatgc gcttaagcgt ctggatgtca gtccgttagg ctgtggcgcg ctggcgggaa
     3841 cggcttatga gatcgaccgt gaacagttag ctggctggct gggctttgct tcagcgaccc
     3901 gtaacagtct cgacagcgtt tctgaccgtg accacgtgct ggaactgctg tcggctgccg
     3961 ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg
     4021 aagcggggtt tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga
     4081 agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggcgcgt
     4141 taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg
     4201 aagacaaaga aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg
     4261 cgctggtgct ggacggcatc caggtgaaac gtccacgttg ccaggaagcg gctcagcagg
     4321 gttacgccaa cgccaccgaa ctggcggatt acctggtggc gaaaggcgta cctttccgcg
     4381 aggcgcacca tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg
     4441 aagaactgcc gctcgacgag ttgcagaaat tcagcccggt gattggcgaa gatgtctatc
     4501 cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc
     4561 agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa catttatatg
     4621 tacaaatttg agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg
     4681 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
     4741 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
     4801 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
     4861 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
     4921 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat
     4981 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
     5041 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
     5101 agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg
     5161 tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac
     5221 ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
     5281 ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
     5341 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagacc acccgtgggc
     5401 gaaccgcgaa tgcgtaccga tggccgatct ggctggggaa aaactgctga tgctggaaga
     5461 tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga
     5521 tacacacttc cgcgcgacca gcctggaaac tctgcgcaac atggtggcgg caggtagcgg
     5581 gatcacttta ctgcctgcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
     5641 tctgccgtgt attaagccgg aacctcgccg cactattggc ctggtttatc gtcctggctc
     5701 accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca
     5761 tttcgataaa gtgttaaaac aggcggttta agccatttag cgcagctacc cgataggctt
     5821 ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
     5881 ctttctgttc cataatcgcc tggccgatat gaataatttc ggcagcgcgc tcgccaaagc
     5941 agtgaatgcc cagaatctct ttcgtttccc gatggaacaa aattttcagc gtgcccacgt
     6001 tcatgccgac gatttgtgcg cgtgccagat gtttgaactg ggcgcggccc acttcatacg
     6061 gtactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
     6121 tgtaaatacc ggtagggata tcttcaatca gatgtgcggt ggcttcacct ttcaccagcg
     6181 cctgcgcggc aatgcgcccc tggtcatagg ccgccgacgc caggctcgga taaccaatca
     6241 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catactgttg accttcagct
     6301 gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatca gtattaccgg
     6361 tgcgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca
     6421 tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtgg cgaatcacta
     6481 cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca
     6541 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
     6601 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
     6661 tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
     6721 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc
     6781 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
     6841 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
     6901 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt
     6961 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
     7021 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
     7081 cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
     7141 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
     7201 aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc gtcattaatc
     7261 tggctatagc gtgagcggcc tttgctttgt ctgcctcctt cttaacccat ttattcacta
     7321 ttaatcgctt tttattatgg ctgatagttt tatcttgccg aagcgtgctt ttttgttatt
     7381 gtctttatga aatcttctta taaaacaaac atataaata
//
LOCUS       DAZGCQ010000190         7257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000190 DAZGCQ010000000
VERSION     DAZGCQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.190"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..423
                     /locus_tag="ACHK07_004880"
     CDS             <1..423
                     /locus_tag="ACHK07_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8105095.1"
                     /translation="SVFMQLELGDKYRSQFFTPWSVASMMAQMQMGNVKALFENKPFI
                     TLSEPACGAGSMILAMADTLNRSGYPAYHRMWVSATDIDPLAAGMAYIQLSLCGVAGE
                     VVIGNSLCNERRRVLLTPGHYLGNWDHRLRHLQEQNVA"
     gene            complement(657..1209)
                     /locus_tag="ACHK07_004881"
                     /pseudo
     CDS             complement(657..1209)
                     /locus_tag="ACHK07_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1326..2231
                     /locus_tag="ACHK07_004882"
     CDS             1326..2231
                     /locus_tag="ACHK07_004882"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8105096.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            2276..3151
                     /locus_tag="ACHK07_004883"
     CDS             2276..3151
                     /locus_tag="ACHK07_004883"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8105097.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            3200..4672
                     /locus_tag="ACHK07_004884"
     CDS             3200..4672
                     /locus_tag="ACHK07_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8105098.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAVLITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            4676..5506
                     /locus_tag="ACHK07_004885"
     CDS             4676..5506
                     /locus_tag="ACHK07_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8105099.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEESSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            5552..6262
                     /locus_tag="ACHK07_004886"
     CDS             5552..6262
                     /locus_tag="ACHK07_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO8105100.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            6275..>7257
                     /locus_tag="ACHK07_004887"
     CDS             6275..>7257
                     /locus_tag="ACHK07_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO8105101.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPADDYFLNNHIISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLA"
BASE COUNT         2038 a         1223 c         1784 g         2212 t
ORIGIN      
        1 agtgtgttca tgcaactgga actgggggat aaataccgta gtcagttttt cacgccatgg
       61 agtgtggcca gcatgatggc acagatgcag atggggaatg tgaaggcgtt gtttgagaac
      121 aaacccttta tcacgctcag tgaacctgcc tgtggtgccg gcagtatgat tctggcaatg
      181 gctgacaccc tgaaccgttc ggggtatccg gcataccaca ggatgtgggt gtcggcaacg
      241 gatattgacc cgctggcagc cggcatggcg tatatacaac tgtcgctgtg tggtgtggcc
      301 ggagaagtgg tgattggtaa ctcgctctgt aatgagcgac gccgcgtact gctgactccc
      361 gggcattacc tgggaaactg ggaccatcgt ctgcgtcatc ttcaggagca gaatgtggca
      421 tgatgcagaa tgttttatca ctgcgccagt cttcggactg gcgttttgtt caactgccag
      481 ttccctgaat gttttatttg ttcaataaag cctgtttatc aataaatttt ctgacgacag
      541 ggaatggtat gtttttatta gtttttatgt gttggacaac aagacagaat aatgatgtct
      601 gttttatgta tacgatatag ccactgattc aacgcattat cggctatatt gccagtctta
      661 tttagtgcca gattcattgt atcgaaggcc gctgccgtca gtagatggct ggtcagttct
      721 atcagacaga ccatttttac ctgaggataa agtgtctggt ttccaccatg tatttggcga
      781 gtgaagcttt atcgtgttct tgcgtgtcct gtgttcgtca gaacacgcca tcaatggtca
      841 gcagattcag atcgcaccag agcggatatg gcgtggtgtt atgccagagc tgcgctgttt
      901 gagtgaacac gcagcgtatg gctccgcttc ctggccgctg gcgggcatgg agcacggcgc
      961 tactgcctgg cagtatgatg tccagccggt tcacaatctg atgaaggttc cttaagctca
     1021 tgggccatac tgataattca ccagaccatc atttccagag gaagacgccg tttgtgtagc
     1081 gtgacagtgt cggactcttc agggctgtgg gtaatcagtt ccagggcaag atagtcgcag
     1141 agggttacgc agagaatagt aactggacac tggatcgatc aaccgatcct taactgagcg
     1201 gcatgacagc tttctgatgg atttttttga taatcttcac attgtgcttc ttttctgttg
     1261 gataattttt gcacttggtg gtgtactagg tatcccatta tgcagttaag tgaaggtgat
     1321 taaaaatgca atgtgaattt aaaggggtca tatcagcatt acccactcct tatgaccaat
     1381 cccagcaaat cgatatggag agtcttcgaa aactgatccg ttttaatatc gaacaaaata
     1441 ttaagggatt gtatgttgga ggctctactg gagaagcatt tcttcagaat gttgcagagc
     1501 gagaaaaaat tcttgaaaca gtagccgatg aatcagatgg aagactgaca cttatagcgc
     1561 atgtgggcgg aatcagtacg gcagaaagcg aggtacttgc aaaagctgca aaaaaatacg
     1621 gatatcatgc tatatctgct gttactccat tttattatcc tttttcattt gaagagcatt
     1681 gtatccatta caggaagatt attgattctg cagatggact tccgatggta gtttataata
     1741 taccggctct tagcggagtg cgtttttctt tagatcagat aaatgagctt gtcacaatac
     1801 ccggggtttg tgcattaaaa cagacatccg gtgatttatt ccagatggaa caaatcaaaa
     1861 ggaatcatcc agagcttgtg ttatataatg gttacgatga aatatttgca tcagggttaa
     1921 ttgcaggtgc agatggggga atcggtagta catataatat aatgggatgg cgttatcttg
     1981 aaatatttga agcagtcaaa aacaatgatg tgattaaggc gaaggaaatg caggtggcct
     2041 gtaaccaggt tattgacact cttattcaga gtggtgtcct ggcaggtata aaaactcttc
     2101 tttactatat ggggattcta aatactcctg tatgcagaag tcctttttct ccggtaaagg
     2161 aaaagaatct ggatgtatta tcaaaacttg cagagcgact gctcgaagaa catgacagaa
     2221 ataaaaaatg aaaataatct gaataattgc aagattgtta tataggtgaa aatgaatgat
     2281 aacactggct gttgatatag gaggaacgaa aatttcagcg gcgttgatat ctgatgacgg
     2341 ttcatttttg ctgaaaaaac agatatcaac acctcatgaa agatgtcctg acgaaatgac
     2401 aggggcttta cgtctactgg tttctgaaat gaagggaaca gcggagcgat ttgcagtagc
     2461 atcaacaggg attattaata atggagtatt gacagctctg aatccagaca atcttggagg
     2521 gctgaaagag tatcctctga aaaatataat ggaagatata actggtttaa atggaagtgt
     2581 gataaatgat gcccaggcag ccgcatgggc agaatataca gttttgccaa aagaaatttg
     2641 tgatatggtt tttataaccg tttctacggg ggttggaggg ggaattgtag ttaacagaaa
     2701 attattaaca ggtgtttctg gattagccgg acatgttggg catatattgt ccggagtaac
     2761 ggatacagaa tgtggatgtg gtcgtcgggg atgcgttgaa gcggtttcat ctggtcgggc
     2821 catcatgggg gcagcaaaaa atgaacttgc aggatatagc accaaatata tttttgaatt
     2881 agcgagacag ggatataagg aagcagaatt tttgacagag cgttctgcca gtactattgc
     2941 tgaattaata gtaagtttaa aattactgct tgattgtcag gttgttgttg tcggaggaag
     3001 tgttggactt gctgatgggt atattcagaa agtgtcgaaa catctttcca tatattctga
     3061 aatttgtaat gtaatgctgt ttcccgcata tttcagaagt gactctggtc ttattggtgc
     3121 aactctttgg gaccgggatt gtattactta aagtaatact ccagtgtatt ataaatccgc
     3181 tattattgtg aggtttaata tggactcctg tattcagaaa aagcaggttt ggtataagca
     3241 tctttcaccc agacagtgga agatattcgg tgcggcatgg acaggttatt tacttgatgg
     3301 ttttgatttc gttctgatat ctctggttct tacagaggtt cagcatgaat tcggcttgac
     3361 gacaatagaa gcggcaagcc ttatttccgc tgcttttatt tcccgatggt ttggggggct
     3421 tgctattggc gctttatcgg acaagatggg gcgcaggatg gcaatggttt taagtatagt
     3481 gctattctct ctcggaaccc ttgcctgtgg tctggctcct ggatatgcag tgatgtttat
     3541 tgctcgcatt gttattgggc ttggtatggc aggtgaatat ggctcaagtg taacatatgt
     3601 tatcgaaagc tggcctgttc atctcagaaa caaggcaagt ggttttttaa tttctggatt
     3661 ttctattggg ggaggtttag ctgcacaggt ttatagcatt gtagtaccac tatggggctg
     3721 gcgttctctt ttctttgtcg gcatgcttcc aatattattt gctttttatc ttcgcaagaa
     3781 tcttccggag tctgatgact ggcagaaaag gcaacaagag aataaacccg tcagaacaat
     3841 ggtggatatt ctgtacagag aaaagaataa atacataaat attcttctgt cttgtattgc
     3901 atttgcatgt ctatatgttt gtttttcagg cgtcactgca aacgctgttt taatcacagt
     3961 tatggcattg tgctgtgcgg ctgtttttat cagttttata tatcagggta tggggaaaag
     4021 atggccaaca ggtattatgt tgatgcttgt ggttatgttc tgtttcctct acggatggcc
     4081 actgcaggct tttcttccta catggcttaa ggttgatatg cagtattcac cagaaactgt
     4141 tgctctcata tttatgctgg caggttttgg ctctgcagct ggaagttgta taggtggttt
     4201 tatgggggac tggcttggaa cccgaaaagc atatgttata tcacttctta tcggtcagct
     4261 tgttattatc cccgtttttc ttgtggaccg ggactatgta tggttacttg gccttctgat
     4321 ctttactcag caggtttttg gtcaggggat tggtgctctg gtgccgaaaa taatcagtgg
     4381 ttattttaat gtggagcagc gtgcggcagg actggggttt atttataatg taggttcact
     4441 tggtggagca tgtgcgccta ttcttggtgc agttgttgcg tctcatacaa gtcttggtac
     4501 agcaatgtgt tctcttgctt ttatcttaac atttgttgtt cttgtcctta ttggttttga
     4561 tatgccatca cgtgtacaga gatggatcca tccagaagct gcgcttgaat atgatactgt
     4621 cgatggaaaa cctttttatg gagcaagaaa aaagaatgtt gcaggagaat aacggatgct
     4681 atcagggaat aaaaaaatga ttcattacag ggagaatata tattctcttg ataattatga
     4741 gcgttacata actgacagtt cgctatttga gaatactgac gatggcataa tatatacagg
     4801 gattgtgccc cttattacag ttgttgagcc tgttgtatct aatcaacata gtgtgctgat
     4861 tatacctgga ggtggataca ggaggattgt ttatgaaaga gagggacttg atgtaggcag
     4921 atggatagcg ggtcagggat atacggcatt tattctttct tacagaatgc cctccgttaa
     4981 tggttattat tcctttgcgg atgcgaagaa tgccctgaac tttatttctt actgggccgg
     5041 aatgtcggag agaacattta agagtgttgg atgtatgggg ttttcggcag gtggtcatct
     5101 tgctggatat attactcatc tccattatga ggaagagagt tctgtgcgtc ctgactggat
     5161 ggctctgata tatccagtta tatctcttga caggagtatt gctcatcagg gatgccggaa
     5221 acgtcttctg gatgcaggtg aaagtgaata ttcagccagt cttgaaaata gtgttacaga
     5281 gtatatgcca ccagtcttta ttgtgcatgc aacagatgat aaggatgttc cagttattca
     5341 ttctctgaga tattttaact ctatccggga taagggacat catgcagaag ctcatttttt
     5401 tgataaggga ggtcatggat ttggtgtatc cggaaaaaat ggatatccga tagaaaaatg
     5461 gcaaagcctg cttctggaat ggcttgcgga acataagttt cattgagaaa tcagattaca
     5521 agtgataaat atatttaatg atgaggcatt tatgaaaaaa tattatccac tattaactgt
     5581 aattcttctt ggtttacctt tgtgctctca ggcattaact cttaatctac gtggaggata
     5641 cagaagcgcc agtcacgctt atgaaacgtt gattcaggtt agtcaggggt ggaataatgg
     5701 ttggtgggca agttttgagt caaacacctg gaatacaatt cacaacaatg aaaatgaatt
     5761 gtttgctctc aattataata aaatagatgt aaataaaaca tataaaatag atgacaaatg
     5821 ggctctaatc ccagggattc agacacaatg gatgtcagca ggaagtcagt ataaccctta
     5881 tctgaaaatt tcgtatcagg caacgccaga tcttaatctg gccttacgct atcgctatga
     5941 ctggaaagct ttccgacaga cggatttaga tggcaacagg gcgcgtaatg atcagcatca
     6001 gtttgatggt tatgtgacat acaaaattaa tgattcatgg ttgtttgcat ggcagaccac
     6061 ggtatatacg aaggttaata atttcaaata tgggaatcat aaaaaaacag caactgaaaa
     6121 tgcatttgtt ctgcaatata aaatgtcacc ggtgtttacc ccatatatag aatatgatta
     6181 cctggataaa caaggggtgt ataaggggaa agataataaa catgaaaata gttaccgtgt
     6241 cggtgttctg attaattttt aaaaggtgat tacaatgaaa gtgaaaaact ttatatatct
     6301 tcccttctgc ttgtttattg gaacgtcggt agccggagct ttaccggaaa ttcctgaacc
     6361 atttaaatat ggtgtaggag ggatcgagaa tggtaaaata tatataggac tgggaagtct
     6421 gggaaataac tggtatatga tagatacaaa ccagagtgaa aaaaaatgga ctaagatagc
     6481 acaatggccg acagttccca gagagcaggc aacagctact attattgatg gtaagatcta
     6541 tgtatttgga ggaattggaa aagataaaag tggggtcata accttacaaa aagatgtcta
     6601 ttcctatgat attgctaaag ataaatggga aaaattgatg acccgtcccc ctgtatctct
     6661 tgccggtcat gtttctttta tccataatgg tcatgcggta tctactggtg gtgtaaatga
     6721 gaatattttt aatggatact tttcagatgt cgagttatca aaagggaatt ccgcgctgac
     6781 agagaaagtc aacagggatt attttagtaa acctgctgat gattattttt taaataatca
     6841 tataatttct tatgacccgt caaaaaacca gtggaagaac ctgggaacga ctccatttcc
     6901 gggaactgct ggttcatcgg tgatttttgc agaacagcaa atttatatac tgggagggga
     6961 gagaaaaccg gggcttcgtt ctgttagatc ctggacgggg gagctttcgc atgatcgaat
     7021 taaatggtct gaacttccac cagtagcttc acccgagggg gtgtcaggag catatgcctc
     7081 tgttattgat ggaaatatct ttctggccgg tggtgcatat ttcccggggg cggcagaaaa
     7141 gtatagcaat ggtgaatact ggtctcataa aggactggat aaagcctatt caaaagagat
     7201 atatcagcta ataaaaaatg actggaaaaa agttggttct ttgccggaag gacttgc
//
LOCUS       DAZGCQ010000191         7068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000191 DAZGCQ010000000
VERSION     DAZGCQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.191"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..902
                     /gene="hlyE"
                     /locus_tag="ACHK07_004888"
     CDS             <1..902
                     /gene="hlyE"
                     /locus_tag="ACHK07_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO8105102.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFF
                     TTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKK
                     MINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1109..1555)
                     /locus_tag="ACHK07_004889"
     CDS             complement(1109..1555)
                     /locus_tag="ACHK07_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO8105103.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1647..2306)
                     /locus_tag="ACHK07_004890"
     CDS             complement(1647..2306)
                     /locus_tag="ACHK07_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8105104.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2378..2671)
                     /locus_tag="ACHK07_004891"
     CDS             complement(2378..2671)
                     /locus_tag="ACHK07_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO8105105.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2683..2841)
                     /locus_tag="ACHK07_004892"
     CDS             complement(2683..2841)
                     /locus_tag="ACHK07_004892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105106.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2912..3313
                     /gene="pliG"
                     /locus_tag="ACHK07_004893"
     CDS             2912..3313
                     /gene="pliG"
                     /locus_tag="ACHK07_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO8105107.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3433..3801)
                     /locus_tag="ACHK07_004894"
     CDS             complement(3433..3801)
                     /locus_tag="ACHK07_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO8105108.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4321..5016
                     /gene="minC"
                     /locus_tag="ACHK07_004895"
     CDS             4321..5016
                     /gene="minC"
                     /locus_tag="ACHK07_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO8105109.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5040..5852
                     /gene="minD"
                     /locus_tag="ACHK07_004896"
     CDS             5040..5852
                     /gene="minD"
                     /locus_tag="ACHK07_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO8105110.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5856..6122
                     /gene="minE"
                     /locus_tag="ACHK07_004897"
     CDS             5856..6122
                     /gene="minE"
                     /locus_tag="ACHK07_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO8105111.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6494..>7068)
                     /locus_tag="ACHK07_004898"
     CDS             complement(6494..>7068)
                     /locus_tag="ACHK07_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8105112.1"
                     /translation="YASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSNGAGVESPWNAVAGVNWSF"
BASE COUNT         1987 a         1699 c         1571 g         1811 t
ORIGIN      
        1 tcgttgcaga taaaacggta gaggtagtta aaaacgcaat cgaaaccgca gatggagcat
       61 tggatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca
      121 taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagttggcg
      181 atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt
      241 atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tattttgcta tttgatgagt
      301 acaatgagaa gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca
      361 tcacgaagct gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg
      421 cttccgggaa actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca
      481 gctatttcca gtcacaggta gataaaatca ggaaggaagc gtatgccggt gccgcagccg
      541 gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgccgcg ggcgtagttg
      601 aagggaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagagt ttctttacca
      661 ccctgtctaa cacggttaaa caagcgaata aagatatcga tgccgccaaa ttgaaattaa
      721 ccaccgaaat agccgccatc ggggagataa aaacggaaac tgaaacaacc agattctatg
      781 ttgattatga tgatttaatg ctttctttgc taaaagaagc ggcaaaaaaa atgattaaca
      841 cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta cctgaagtct
      901 gatcagcgat tattctctcc atgtactcaa ggtataaagt ttatcacatt taaaaataca
      961 gacgtttttt aaagattgag agttacctgt tgataataag gtatcgggta actctcatta
     1021 tgacagagcg ctttcaaaaa cagggctacg aaatggcgag catccagacg tatattaaga
     1081 aagcccccat caacatctat taaatttatc actgcgccca gtcaggttta tttaagatat
     1141 gatcctgcca gtcaatcact tctgattctt tcactgcgat atgacgcaca gagatacgtt
     1201 caccatgcat tgccgctttc gaaccagtaa gtagcggatg ccacgcaggt aaatctttac
     1261 cttccgccag caaacgataa gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt
     1321 cacgggttaa tttaatgcag tcgggttcaa actcgaaacg acgttcgtag ttccgacatt
     1381 gacaggtttt aatattgagc tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt
     1441 cttcatccat cagtttatgc aggcaacact gaccgcaacc atcacacaac gactcccatt
     1501 ccgcatcgct catttcgtcc agggttttac tttgccagaa aggtacatcg ctcatcaggt
     1561 gttccgccat tacgttaaaa cgcaccttat aaccagtctg gcacagcgat gcaagttttg
     1621 ccgccgcttt caggcggcaa aaagtattac aaaacgcgag ttgtcaaaga atgcccatcg
     1681 aaagtgactg tcagctcatc accgctttgc aacgggccga cgccatcagg cgtgcctgtc
     1741 agcacaacgt caccggcctt gagggtaaaa aacttgctca tataagcgat cagcggaacg
     1801 attttatgga tcatgtccgc agtcgtacct tgctggcgtt gttcgccgtt tacgctcagg
     1861 cccagcgttg tattttgcgg gtcgccggta aattccgccg cgggaataaa cccggaaagc
     1921 ggacaagagt tatcaaacgc tttagccttt tcccacggct gcccggcttt cttcattttt
     1981 ccctgaacat cacgcaatgt cagatcgagc gccacgccat aaccggcaat ggctttgcgg
     2041 acatgctctt ccgtagcctg acgcagcgtc gcgccaatca acaccgccag ttcgacttca
     2101 tgatgaactg aaccgaaatc ggatgggatc gccaatggct gccgcagatc gcacagtgcc
     2161 gtttctggtt taataaacag cactggctct tcgggcactg cgctgcccat ctctttaata
     2221 tgtttggcat agttactgcc aacacagact actttactca ccggataatc cagcagcgca
     2281 ccttgccagt tgtgatgttg atacataacg ttcccctcac cttatcgtgg atatgaaaac
     2341 ccgcgccgac aaagataatg ccaccgccgg atatcggtta tttgttagtg tcgtctgttt
     2401 tctgccccat gacggaaaga tgttgcttca gcaaatcttc gggtggtggc ggtaactgca
     2461 aatagtaacc ttgctcggtt aatgcctgtt taaccttttc aatatcggca ttgaccagtt
     2521 tcttccgccc atccagcggc agaatcatcg ctaactgagg ctgaccaaaa cctttcatca
     2581 gttcctcagg aacacgcgaa aaatcgtctt ttttttcgac atataaatag gtctggtcac
     2641 gcttgctgct tcgataaatc acacaaaaca tacttttact ctttaagatg ccaggttttg
     2701 gcataagcta acatcaatga aacccgcgcc acaagccagc cagctgtaga tttctacggt
     2761 ttgttgagtc catgcccctt tataagtaat ccccccggca aaaatgccgc gacgggattt
     2821 taccgaacgc aggaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt
     2881 catcgtactc attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt
     2941 attgctgctt gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga
     3001 gttcagaaaa ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga
     3061 tacatatacc ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg
     3121 tgctgatact tacctcttcg ggccaggaat cgatgattct gttgatttat ccagatattc
     3181 cccagaactc gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg
     3241 agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat
     3301 tcaaattaag taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac
     3361 tcctctctgt cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat
     3421 gtaaacaagg catcactcac gacacataaa taacatcttt gtggtggttt gattgatttt
     3481 tccactccgc ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac
     3541 atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg
     3601 taagttttca gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc
     3661 tgagttcaca caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc
     3721 agcgtcatgt ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata
     3781 aaaaatacgc atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc
     3841 atttcatcat caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag
     3901 aagtatcaga cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat
     3961 aacgggtatc tgttctttaa tgatgctgca aaaaatggaa agttaagaaa gcctggcatg
     4021 gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctaaaga tgctgtcacg
     4081 attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt
     4141 tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt
     4201 tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta
     4261 tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg
     4321 atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg
     4381 catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc
     4441 gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac
     4501 tggtcagcga tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc
     4561 tgcaaagatg cgcaacttaa agccgaaatt gaaaaaatgg ggctgcctat cctgacggaa
     4621 ggaaaggaaa aagcgccacg tccagctccc gcaccgcagg ctccagcgca aaatacaacg
     4681 ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct
     4741 ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt aattgccgat
     4801 ggtaacattc atgtctatgg catgatgcgc ggtcgtgcgc tggcaggggc aagtggtgac
     4861 cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa
     4921 tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta
     4981 gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta
     5041 tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg
     5101 cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata
     5161 tcggcctgcg taatctcgac ctgattatgg gttgcgaacg ccgggtcgtt tacgatttcg
     5221 tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg
     5281 aaaatctcta tattctgccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag
     5341 gggtcgctaa agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact
     5401 ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta
     5461 ttaccaccaa cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg
     5521 cgtcaaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa
     5581 cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg
     5641 agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct
     5701 ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag
     5761 ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga
     5821 aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct
     5881 cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac
     5941 gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg
     6001 tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag
     6061 atggcgatat ttctattctt gagctgaacg tgaccttgcc ggaagcagaa gagctgaaat
     6121 aggcccgctg taaaagcgca tttatcttca aggcagagtc atctctgcct tgagtttttc
     6181 atccctctca tcgacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct
     6241 atttccacct ccctctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg
     6301 cctggttgag taaataacct tattgttagt gtggatatac tgggagatga taaccccgca
     6361 atttcattat tcattgttct cccataggat gaggtttccc gccgtaactg gcgggctttt
     6421 tttgccccag aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt
     6481 taatcgttgg tggtcagaac gaccagttca caccagccac cgcgttccac ggggattcca
     6541 caccggcacc gttgctatac cccaccccga gatgcccgct taacgtactg ctgaatgagg
     6601 ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt
     6661 cactattcac tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcagcc
     6721 acggctcaac ttccataccg tttcccagac gcatgttgta actcagcgtt gcgcccagtt
     6781 cacgatatat actgcgggta tcgactgatt tcgattccat gccattggat aaatgatatt
     6841 cggggttatc agcggtgaac cccgttaacg atgcatacgg cgtcaggttc cagttaccat
     6901 cggtaaatcg catcccggtt tcaatgtgac cgcccagccc gttgctgtgg taactgccat
     6961 tggcggctcc accgctgctc attttaccgg ctacgttact ttcaaaacgg ttcagcttca
     7021 cgacaccgtc cagatagaaa ccactttcat gttcccaact ggcatagc
//
LOCUS       DAZGCQ010000192         6817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000192 DAZGCQ010000000
VERSION     DAZGCQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.192"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..368)
                     /gene="rcnR"
                     /locus_tag="ACHK07_004899"
     CDS             complement(96..368)
                     /gene="rcnR"
                     /locus_tag="ACHK07_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO8105113.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            489..1313
                     /gene="rcnA"
                     /locus_tag="ACHK07_004900"
     CDS             489..1313
                     /gene="rcnA"
                     /locus_tag="ACHK07_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO8105114.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVRAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1532..1870
                     /gene="rcnB"
                     /locus_tag="ACHK07_004901"
     CDS             1532..1870
                     /gene="rcnB"
                     /locus_tag="ACHK07_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO8105115.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1952..2986)
                     /locus_tag="ACHK07_004902"
     CDS             complement(1952..2986)
                     /locus_tag="ACHK07_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO8105116.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYGELSAQHNLVVQGDFALTQNQ
                     TATHTYTFNDTSCVSTNTVAQIDPSDIVIGLYNDTIKLKLHFEWEKKGSITLSNNQTS
                     FTSGYNVTVTSAASDAIVNLSGGGSGSLMINSVATLSSASSSTRLAAFLQFAGCFLGG
                     TSWDICVQNYKNALSKNAGVYSFNLALSYNPITTTCKPDDLLITLESIPILQLPTTGN
                     KATINSKQGDIILRCKNLLGQQNQTSRRMQVYLSSSDLLAGSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(3000..5479)
                     /gene="yehB"
                     /locus_tag="ACHK07_004903"
                     /pseudo
     CDS             complement(3000..5479)
                     /gene="yehB"
                     /locus_tag="ACHK07_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(5495..6169)
                     /gene="yehC"
                     /locus_tag="ACHK07_004904"
     CDS             complement(5495..6169)
                     /gene="yehC"
                     /locus_tag="ACHK07_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8105117.1"
                     /translation="MKGLSSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(6249..6791)
                     /gene="yehD"
                     /locus_tag="ACHK07_004905"
     CDS             complement(6249..6791)
                     /gene="yehD"
                     /locus_tag="ACHK07_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8105118.1"
                     /translation="MKHSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSEVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DSSVNSGDVLSSLVMRVAQD"
BASE COUNT         1871 a         1622 c         1343 g         1981 t
ORIGIN      
        1 gcatactaat aggtaaaatc aatatgctta gatatacttt caacgaataa cactatctgt
       61 ataatgctct gattggtgat agttataact cctatttatt tgatatatga atccagcacc
      121 ttcagaacga catccagatc ttcttcacgt tttagctcat ccccctggtg aacgatgtgt
      181 tccgtcagat gacctttaat cacttcccgc atcagaccgt ttaccgcgcc acggatagca
      241 gcaatctgtt gtaaaactgc agcgcattcg tgcggctcgt cgagcatttt cttgagcgcc
      301 acgacctggc cctgaatctt actggcacgc gctttcagtt tctgtttatc acggattgta
      361 tgagacatgg caacacctgg ttaacaagaa tatgaaaaat catagcacta ttaatctact
      421 ggggggtagt atcaggtact gggggggagt agaatcagat tgccgaatta atactaagaa
      481 ttattatcat gaccgaattt acaactcttc ttcagcaagg aaacgcctgg ttcttcatcc
      541 ccagcgccat cttacttggt gcgcttcatg gcctggaacc agggcactca aaaacgatga
      601 tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca ggcggtaatg ctcggactgg
      661 cagcaaccat ttcgcatacc gcagtggtct ggttgattgc ctttggcggg atggtgatca
      721 gcaagcgctt tactgctcaa tcagcagaac cgtggctcca gctgatttcc gcagtgatca
      781 ttattggcac cgcgttctgg atgttctggc gtacctggcg cggcgaacgc aactggctgg
      841 agaatatgca cgagcatgat catgaacatc atcaccacga tcacgaagat caccacgacc
      901 atggacatca tcaccatcac gaacatggcg agtatcagga tgcccatgca cgagcccatg
      961 ccaatgacat taaacgacgc tttgatggta gagaggtcac caactggcaa attttgttat
     1021 ttggcttaac cggtggcctt atcccctgcc cggcagcaat taccgtgctg ttgatatgca
     1081 ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt cagtttcagc attggcctgg
     1141 cgttaacgct tgtcaccgta ggcgttcgcg cagcaatcag cgttcaacag gtcgcaaaac
     1201 gctggagcgg atttaacact ctcgctaaac gcgcccccta tttttccagt ctgttgattg
     1261 gcttagttgg tgtgtatatg ggcgtacatg gcttcatggg cataatgcga taactcaatc
     1321 ctttaggctt gcatgttatc ttcagcccag gatgattctt aaatcagcta tttccgctga
     1381 ctctctgctc gataaggatg cttttagggc atcctttcat ttacactttt tacgaaatca
     1441 tgggatcact aacaaaatat cgcttgtcag ttatattgta tggcaggaaa gatatgcgac
     1501 tgatattaca gatccccaaa gtggagagtt tatgaccatt aaaaataaga tgttgctggg
     1561 tgcgcttttg ctggttacca gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa
     1621 tacctcggga atttctaagt atgagttaag cagtttcatt gctgacttta agcatttcaa
     1681 accaggggac accgtaccag aaatgtaccg taccgatgag tacaacatta agcagtggca
     1741 gttgcgtaac ctgcccgcac ctgacgccgg aacccactgg acctatatgg gtggcgcgta
     1801 cgtgttgatc agcgacaccg acggtaaaat cattaaagcc tacgacggtg agatttttta
     1861 tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg cagacacctt gttatttttt
     1921 attattagcc acttgctcgt cttgcttgtt attagtcgta tttcacgttg attaatgcgg
     1981 ttgcctccag tgcgccagat ttaattttat ttgtatcgta gacgtagtaa ctggcagtta
     2041 tcggaattga aaccgttgta tctgtaagtt ttgactttgc cgcaatttct tttaaatttg
     2101 tgtatccttt actgctgtta gtgatattta aaagtgtgac tggcgaacca ttactttcaa
     2161 gaataaatcc gactccatta tcttctgcac ctttcaggat cgtgttactg ccggctaaca
     2221 aatcagaact tgataaatac acctgcattc tccgtgatgt ttgattctgc tgacctaata
     2281 aatttttaca gcgcagaata atatcccctt gtttactatt tattgttgct ttgttacctg
     2341 tggttggtaa ttgtaaaatt ggaatactct ctaaagtaat taataaatca tcaggtttgc
     2401 aggtcgttgt tatcgggtta tatgacaatg ccagattaaa ggaatagaca cctgcatttt
     2461 ttgataatgc atttttatag ttttgcacac agatatccca tgaggtgccg cctagaaaac
     2521 aacctgcaaa ttgtaaaaaa gcagccaaac gagtagatga tgaagcactg cttaatgtcg
     2581 cgacactgtt aatcatgaga gatccacttc ccccaccaga caaattcact atggcatcgc
     2641 tcgctgccga tgtcacagtg acattatatc cactcgtaaa actagtttgg ttatttgaaa
     2701 gcgtgatgct accttttttc tcccattcaa aatgcaactt aagtttgatg gtgtcattat
     2761 agagtccaat cacaatatcc gaggggtcta tctgcgctac tgtattagta ctgacgcaag
     2821 aagtatcatt aaaagtatat gtatgagttg ctgtttgatt ttgagttagc gcaaaatccc
     2881 cctgaacaac caaattatgc tgtgctgaca actcgccata acatgcagca taactcacag
     2941 gcataaccat catcattact ataaataaca ttatacgaat attcataacc acctgactat
     3001 tactggcaaa tataattcct actttcatca atctctttac cgaaggtgat tgtgcatgaa
     3061 agtccttgtt gcttatcaat tgctacatta accgatggcg gtacttcatt ggtgcgaata
     3121 aataactgac ttccctggcc gacaacgcca atattatgac catggatatc attgacttca
     3181 taaccaaacg ttaatggttg cccatccgct cttaacgctt ttataaacca gggcttgcgc
     3241 tgatcggtat caaaattaac cagtacaacc gcgccgcgat aagaggcggc aattttccgg
     3301 ttaccgcgca attctgcttc gctatcgctt tgcgacacat ccagcatcag gtgattttcc
     3361 cgataaggtg tcattccgtc gtataccacc actccattac ggtttgttgt gcgatatttt
     3421 tgcccattga cataagcatc tttaattcct ggcgcattca tcacagcaaa cgtttcggaa
     3481 agacggttcg ccagattaac gccacccgac caggcgacaa tgccccctga aacactggct
     3541 ccagcctgtc gataagtact cgactgacta taactgccat tcactgtcgc aaccggcgcg
     3601 ttccaggtta aattcgctcc agctgtcgtt tcatttccct gatgttgatg actcaggttg
     3661 acaccataat tgaactgatc ccgactccct actgttcctg ataatcccgt attatttgag
     3721 gcaaacccct gatcatcaaa cgtcgttgag ttagacatat atatttgccg acgaggcgtc
     3781 gaaacgtcat caccccaatc aaagggaatc gatataaaaa tattaaaacg tttctcttca
     3841 tgatgattct cgtcataagc ctggcttgcc gcgagggtat agcttatccg tcgcaggttg
     3901 ttggaataac tcaactgata atccttacta ctgccgctac gcccccagta atctcgccat
     3961 aacgtactta atgacacaga accccagcct tctggcaatg actggctcat attggcagaa
     4021 aagctatttt tacggccaaa atcgttctgg taataatcgg caatgtcata gacatcgttt
     4081 tcatcacggc gataattatc tttattgtta gcccaaacgt gatcattaaa tgtccgataa
     4141 tcacgcgacg aataacgcag gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt
     4201 tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtttactat
     4261 gcgacttcgt ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag
     4321 tgaacgcgta ataattattc gcgaccatcg agccaccata cagcgtcaat aagttattaa
     4381 aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc
     4441 tacggcccgc cgcaaaatca tatttcgaca cgccgggttg cagcatattt ggcacggctg
     4501 cataaggcac cagataggtg gttaccgaac cgtccgcctc tttcacgctg acatcaagat
     4561 ctgctccacc accggccaac tgcaaatctg taatcgcaaa cgggccagga ggaacctctt
     4621 tctgataaac cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc
     4681 cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca
     4741 accgcacacc tctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca
     4801 cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc
     4861 acgcccctgg attgttattt gttttactga aactggcatc agaatgcagt tgccacccca
     4921 gtaaatttaa cccgctgtta aaacgtacat atgtactctt actattaccc gacgctttat
     4981 agtcactgta atactgactc acataataag aggtataaaa cgcattaata ccccgctccc
     5041 agttttccgg tggaacatag ccactttcca gttcttccac ccaggcctgc gggacactga
     5101 aatcgagacg aaaaaccccg atatcccagg tatagctccc accctgaaca agttgctcaa
     5161 atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac cgcttgataa
     5221 tttcccttga taaacatgtt tcttgcgggt tgtctttaac aatgatctca tatttcccgc
     5281 gccattgctt attgacataa atatcgatgt catactgccc cggcaagggt tgattatcat
     5341 caagacgaat atttgcaacc tgctggtctt tcattccacc tatcataaaa tgggtatcaa
     5401 aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa
     5461 gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc
     5521 catggtcatc gataatggtc agatgccagt tatttgcatt attacttttg acattaacac
     5581 tctgactttc taagggggca atcataatag tttcataatt gactttgaca ttattagctt
     5641 tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaat ccattgccac
     5701 tgcgatttac cagtaacttt ttaaaggtcg ctttatttac cggagcaata cccgccggcc
     5761 ggtaaaacaa tttgattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt
     5821 ctggactatt tggtggaata tccagaacat tcagataaaa aatgctttct ttattagtgg
     5881 gcagtttatt cggcataatt ttgattttta cttgctgccc ggaatttgcc cctatttttg
     5941 ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat gacgtatcgc
     6001 catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca
     6061 tcacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca attccggcat
     6121 gtgcaggaag gaccatagaa aaaatgagta aagatgataa tcctttcatt ttaatgcctc
     6181 ttaaattaaa aggccgccat gggatagcgg ccatgtttat tacctggaga aacttacaat
     6241 tttatttatt aatcctgcgc cacacgcata accagagaag ataacacatc acctgaattc
     6301 actgagctat ctgtcagttt caggtatttt gctttaaacg gaatagcata ttgaccttcc
     6361 gctgttttat aactctcaat attaaaaggt gcagaaacat ccaggacttc gctgttatca
     6421 cctaaataga gatagtaacc cacagtatcg ttgctactgg ctatcgcctt atcgttacca
     6481 gttattttat cgttggtaat caacgagagt tttacaccag caacttcttt cgggcagcta
     6541 atttttaaca tgctgctggt tacaccgact gcatcatcat aggtagaata tttagcttta
     6601 ccttcaaaga cagtggttgg tacagttggc atattaatac ttactgaatc accacccgct
     6661 tcgaatttac acggagattc tgcaatattt cctttaatgg ttaatgttcc ggtatcaacg
     6721 tctgcagcaa atactccagc gctcataaaa aaagaagaaa agacagcggc agcaatgatt
     6781 gaatgtttca taatatgttc cttaataaaa tttattt
//
LOCUS       DAZGCQ010000193         6787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000193 DAZGCQ010000000
VERSION     DAZGCQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.193"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHK07_004906"
     CDS             complement(182..667)
                     /gene="sixA"
                     /locus_tag="ACHK07_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO8105119.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVTS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHK07_004907"
     CDS             complement(870..3014)
                     /gene="fadJ"
                     /locus_tag="ACHK07_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO8105120.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCESLVEMGARGESFWKTTATDLQ"
     gene            complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHK07_004908"
     CDS             complement(3014..4324)
                     /gene="fadI"
                     /locus_tag="ACHK07_004908"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO8105121.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SNLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4505..4789)
                     /locus_tag="ACHK07_004909"
     CDS             complement(4505..4789)
                     /locus_tag="ACHK07_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO8105122.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5161..6501
                     /gene="fadL"
                     /locus_tag="ACHK07_004910"
     CDS             5161..6501
                     /gene="fadL"
                     /locus_tag="ACHK07_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO8105123.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
BASE COUNT         1715 a         1798 c         1601 g         1673 t
ORIGIN      
        1 gtaaaaatac attacaactt agtctcattt cgtcagaaat taatagggaa gcagaggaaa
       61 aagtgcgatg aattctgtat cgaagcgtat caactccgtg aaaggttttg agtgaatatc
      121 tactattgca aaaagacaaa tgcccgatgt aagctgtaca tcgggcattt tttcaaacga
      181 atcagatagc tttcgccatc ttcaaattac atgggctcat ctgccagttg aactcgcctt
      241 ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt
      301 cgcccggaca taactcggcg accagatacc caactaacgg taagtgggag ataaccaaca
      361 ccgaagtaac accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc
      421 cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga caatccccta
      481 cttcctccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca
      541 ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaacca ttagtagtca
      601 gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa
      661 cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag
      721 agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt
      781 tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca atccgccatt
      841 cagcacggat tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa
      901 aaactttccc cacgcgcacc catctcgact aaactctcgc aaggggtaaa acgtgaacca
      961 tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata
     1021 tagcggaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc
     1081 ccgtcacgca cgctacggat aacctgctca tcaacacaac gtactgcttc attcagcatc
     1141 aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcacca
     1201 atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa
     1261 agatagaaac cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca
     1321 acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta
     1381 gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg aaaaccaaat
     1441 ttcactagcg cggcatcaat gtgctctacc cgttcacctt cggtcagcat gcggatagct
     1501 tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca
     1561 atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgcgctgat
     1621 gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc
     1681 aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat cggtaaagat
     1741 gacgtgttcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatc
     1801 tgttgtttta attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag
     1861 ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg
     1921 agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt
     1981 atgccctgcg ggttgatatc tttaattctg accggaagcc ccgctttaca agcagtgaca
     2041 taagcaatac cgccgcccat caagccacca cctaaaatcc ccacgctgtt taatggcgca
     2101 ggcggcgcat cactgccggg atctttcttc acttccgtac tggcaaaaaa gatactacgc
     2161 aaagcttgcg attgtggcgt catcgccagt tcgccaaacg cccgtgcttc agcgtcatag
     2221 ccactgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc
     2281 ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca
     2341 cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga
     2401 tccttctttg ccagctcaac agcggcttcc agcagaatgg agtgcggaac aacgtcatcc
     2461 accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag gatcatctct
     2521 aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac
     2581 aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga
     2641 ccgtggcacg cgagcgccag ctccagccca ccgcccaggc aagcgccatg aatagccgcg
     2701 ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccaga
     2761 gcttccgctt cttgcgccgt tttgcagttg ccgatcatgt tgatgtcagc tccggcgata
     2821 aagttgtctg gtttcgcgga aataaatacc acgccgcgca actctttgtt ttcgcggagt
     2881 tgcttaataa tggcgcgcac ctgcgaggca aattccgcct tcagggtatt cattttctcg
     2941 cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
     3001 gatgccattt ccattattcc gcctccacaa ccattgccgc gccaagccca ccggcagcac
     3061 aggcggtcac taaaccaaat ccaccgccgc ggcggcgaag ttcatgcaat gtctgggtta
     3121 tcattcgcgc gccggtcgcc gcaaagggat gcccgtatgc aatcgagcca ccaagcacgt
     3181 taaatttgct atcgtccact tcaccggtgg catgcgcacg ccccagtacg tcgcgagcaa
     3241 aacgttcgct ccccagtagc tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca
     3301 tatcgatcaa tgtcagattg ctcatcgtca aaccggcacg ctccagcgcc agcggtgttg
     3361 accaggctgg accgagcaac atgtcctgcc agacatcgat agcggtaaat gcgtagctgc
     3421 gcagatatcc cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     3481 ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt ccgtgtttac
     3541 gatcaaacgc cggacgcagc tttgcgtaat cagcaagcga ggaattaccg cgaatattgt
     3601 tgtcttccgc gagcagttgt ttataaggag ggataaaggc agtcatcacc tcttctttga
     3661 gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt
     3721 gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg cccatccgca
     3781 agccggtaga atattctgct accgcaggtg gtacgggcat taagtcgcgc aaacgcaggc
     3841 gagagaagag tttcagacgc tggctcattg tgcgggcttt gttgacatca accagcacgc
     3901 gcgccagttt tttactgacg ccaattggca ataccgaaga ggaatctgcc ccaccggcaa
     3961 tccccgctcg aatagttccc gccatcaggc tttctgcgac gtttgcaacc gcctggaaac
     4021 tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt ccggtaccga
     4081 gaacaatttc acgcgcaatg ttgggggctt caggcatttg tacgacctga ccaaagacca
     4141 gttgttcaat cacttcagcg gggatctcgc tgcgtgccag cagttcgcct accaccatct
     4201 tccccaaatc aacagcagga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac
     4261 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac
     4321 ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaacc
     4381 atttttttac atttagccaa gcggagaaaa gggaaagtgg gagctatgac acagagagaa
     4441 aagaagaaga gaaaagaaaa cgcccctgcc agcaattgac aggggcgtac gtacccgaga
     4501 ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat
     4561 cgatatccaa gcgcacacca tcagactctt cagtgaaagt cggggagatt ttgcacggtt
     4621 cggattccac gctacgggct ttctcagtca gcgccgccag cgtttgttct gcttctgcgc
     4681 ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa
     4741 catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc
     4801 tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt
     4861 gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg
     4921 atctaaaaca aaattcaccc gaatccatga gtgcgccacc tccaaatttt gccagctgga
     4981 tcgcgtttct tagatcatat ttgaaaaaag atagaaacat acttgcaaca ttccagctgg
     5041 tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc
     5101 gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc
     5161 atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc
     5221 acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg
     5281 gcttattcag gggaaggcgc aattgccgat gatgcaggta acgtcagccg taaccccgca
     5341 ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgatccggat
     5401 gtagatattt ctgggcgttc cgatttaacg ggccagcgtg ctgatgcaga taatattgca
     5461 ccaactgcgt gggtgcctaa cctacatttc gtagcaccta ttaacgatca gtttggttgg
     5521 ggggcttcta ttacctcgaa ctatggtctg gccacggaat ttaatgataa ctacgctgct
     5581 ggaatctatg gcggtaaaac cgatctgcag acgatcaacc ttaaccttag cggagcctac
     5641 cgccttaaca atagctggag ttttggtctt ggttttgatg ctgtatacgc taaggcaaaa
     5701 attgagcgta ctgccggttc actcggtcca ttgctttccc aacaactgat agcgcaaggc
     5761 gtgccacaaa gttggacaaa cggcatcaat acaccggatt cgcaaattgc tcatctcaag
     5821 ggtgatgaat ggggattcgg ctggaacgcc ggtattcttt atgaactgga taaagataac
     5881 cgttggggcc taacctatcg ttcagaagtg aaaattgact ttgaaggaga ttacaaaagc
     5941 tccatcaacc caaatctgaa caacattttg ggtagcatgg gtttaccata tggcacaatg
     6001 ggtgcaaccc aaaatgggtc cttaacgctg aatttacctg agatgtggga aatttctggc
     6061 tataaccgtg tcgcaccaca atgggctatc cactatagtc tggcgtatac cagttggagt
     6121 caattccagg aactgaaagc gaaaggagac tctggtcaaa cgctgttcta taaagacgaa
     6181 ggtttccggg atgcttaccg tatcgcgtta ggtacaacct actaccacga taaaaactgg
     6241 accttccgta cgggtattgc attcgatgac agcccggttc cggcagataa acgttctatc
     6301 tctatcccag atcaggatcg tctgtggtta agtgcaggtg cgacttatgc atttaatgaa
     6361 gatgcatcca ttgacgttgg tgcatcttat atgcacggtc agaatgtgaa gtttactgaa
     6421 ggtgaagggc cagcagcata tacctttaaa tctgaaggta aagcctggct gttcggtact
     6481 aactttaact acgcgttctg ataacgcgtt cgcctggata aagtcacctg ctatgcaggt
     6541 gactttaact cccccacttc accgaaagta gtttcctcat tgcttgcctc ctgagttttg
     6601 caaacagcct gatggcagct tgccctttca aatcagtaag tggtatccac agacaaacct
     6661 gaagggaaac gcatattttc aggcgttctg ctcgtccttc tcaaagagtt tacttttctg
     6721 aatttccagg atactcaacc ccctggctat tgtgcgctca tacactcaaa ttaaagatag
     6781 gttctaa
//
LOCUS       DAZGCQ010000194         6767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000194 DAZGCQ010000000
VERSION     DAZGCQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.194"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..1342)
                     /locus_tag="ACHK07_004911"
     CDS             complement(32..1342)
                     /locus_tag="ACHK07_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO8105124.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(1355..2626)
                     /gene="dcuC"
                     /locus_tag="ACHK07_004912"
     CDS             complement(1355..2626)
                     /gene="dcuC"
                     /locus_tag="ACHK07_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8105125.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            2762..3679
                     /locus_tag="ACHK07_004913"
     CDS             2762..3679
                     /locus_tag="ACHK07_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8105126.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELAAARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQIMIEYFQQVIQRYDWNG"
     gene            complement(3928..4095)
                     /locus_tag="ACHK07_004914"
     CDS             complement(3928..4095)
                     /locus_tag="ACHK07_004914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105127.1"
                     /translation="MHASPVMTMVVCPTVLLCQNGHQKDQYRESLLSQMIASGHKELT
                     RVWQMNEIQAT"
     gene            4244..5077
                     /gene="iolR"
                     /locus_tag="ACHK07_004915"
     CDS             4244..5077
                     /gene="iolR"
                     /locus_tag="ACHK07_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFO8105128.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(5130..6563)
                     /gene="iolT1"
                     /locus_tag="ACHK07_004916"
     CDS             complement(5130..6563)
                     /gene="iolT1"
                     /locus_tag="ACHK07_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFO8105129.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         2066 a         1426 c         1390 g         1885 t
ORIGIN      
        1 caggagtgat tgacaggata cctgtcagca atcaatacaa ttcagattcc agttttccgt
       61 tccctacaat tttatttagt ccgggagagt tcaatctctc cagacataac gaaaacagtt
      121 taacgcctgt atccagcaca ttttcatcaa aatcaaaatc aacttcgtga tgaccagctt
      181 ttaaatcact gccaagtatt atatatgctg cttttcctcc tatcttctgt acacggcgaa
      241 taaggagcgt tgcatcttca ctgcccccaa atgatttctc tgtcaaagac atgttcatat
      301 cttcttgtgc tatatagtgt atcagattga caagtaaagg gtcattatgt aaatccgttg
      361 cttcaccaac cactttgtac tcaaaacgta aaccaaatat ggaagaaata ccttgtgcta
      421 ttttcagagc atgttcacgc atgtattgat ttattgtttc cgtttctcca cgaacttcaa
      481 gttgcatcct tgcaaaagac gggattatgt tccttccttc acctgcgttt agcgtgccaa
      541 tatttatccg ggacatacct tcaccatggc gtggaatggc aagtaattgc gttgcacagt
      601 gacaagcagc tgccagagca ttatttccca ggtgaggatt tacaccagca tgtgccggag
      661 taccatagaa aaagaaatcc atttttgtcg tacacaacaa atctgtaggt gaagcgacaa
      721 catttcctgt aggaactccc ataccaatat gtgaagaaag gaagtagtcg cagtcatcaa
      781 gaataccacc ttctgcaata ggttttgcac cgcgaacgcc ttcttctgca gcctgaaaaa
      841 ttagcttaat tttcccgcat aacttatggt tattttcaga tacccattgt gccaatccta
      901 aaccaatcga tatatgacca tcatgtccac atgcatgcat taagccagag tgtatggatg
      961 caaatcccat tttttcagga atatgatctt tcgattttga ttcacttacc tgtacacaat
     1021 caatgtcaaa acgaaacgca gtgacagctc cattttttcc tgtatcccat aaagcaacac
     1081 accctgtaaa gccatccatt tcatccagca attcatgggg aacgcctgaa tcaactgcac
     1141 gttttatact atctgcaact tcagttgcat tacggcccat aatatactct gttgacaggt
     1201 atttattccc cgtaataacc tgccatccca taccacgcag aatagtaatt atctttgacg
     1261 ttgtgataaa ttcgcaccac ccactttccg gaatatggtg aaattctctc cgccattttt
     1321 gtaactgatt cagttcagac atatacgcct cctgttaaag gaataataat gcaataaaca
     1381 ataccgtcac aatcattccg ggggcagtgc gttttgcaat ttcaactgga ttaactccag
     1441 ccagaccggc acagataata gttgcaccag ccaacggaga catgtttctc cctagcgcag
     1501 aagccagcat aactgcagca cccagagtat ttgtatcgta accaaaattg ttcgcatgtg
     1561 gtgtgacaac ttcgttgaat gcgaatgcag tcgcatcccc tgtgccggtt ataagtccca
     1621 tcaggaatgg ccccaatgta ccgccccagc gggcaaattc tggtgagtca gtaagaaatc
     1681 tgataaacac atcaatgata ccaacagatc ttaaaccttc aatgaaaaca gctgcggaaa
     1741 ttatgatccc aagaatattt gcatatgatg tccccatacc ctcaaagaag ctggaaacta
     1801 attctttggg attggattta ctgacaatca gggcataaag acaaccgaga atcattgcgg
     1861 ctggaacggt tattttagtc tctccaagaa aaccaaattc agccagcata agtaatgcta
     1921 cgggaagaaa tggtgctaca gatttcagat aaaaacagat atcacggtca gtgaactcaa
     1981 cctgtgtttg ttctgatatg tttcctgcag aatcacattt tgtttctttt cttacaatag
     2041 caacaactgt aagaataaca gcacatatta caccacatgt tattgtagtc ggagtatgga
     2101 atacgacgaa ttccattaat ggtgtttgtg atattttaga tataaatgaa ttatgtgcaa
     2161 caccgggatt gaggtaagca ccgatagttc ctgaaaggat agctgctccg gctattgcag
     2221 gatgaatacg tgctgaaata agtaatggta tcagcgttgc accaacagca gctgatgctc
     2281 cagcagcaga tggaatcgcg atgcaaacaa taaaactgac ggtaaccgca gcaggaataa
     2341 gaaagatacc aagacgagat aatcctttcg ttagaaaatg aaccaggcat gtatcacaac
     2401 cagtgaattt cataacacag gcaaagccca tactggtaca aatggcctga atgagaccgg
     2461 actcagtcat tctgcttgca aatgccgaaa atgcgtttaa aggtgacatg gcaactatac
     2521 atagcagaat acctgcacca attagtgtcg ttcgtgtgtc gtatcttttt atcaaaagca
     2581 caactgtgat tatcagaatc acacatccag ttatcacgtt aaacatgaga tcctccttct
     2641 tcaaagaagg ataatcttta ctttatgtga tttgctcaaa tattgatcat gaacaaaaaa
     2701 atcaattaac cggggatact attatgatgg aggtgataac agaaaattat caggcgaaaa
     2761 aatgaaatat atgcctaaac ttcaacaaat taaatttttc aatgaaatag tcctgtgcgg
     2821 aagtttaaga gctgctgccc gtaaaatgaa tatatctcag ccagcattaa gcagaacgat
     2881 aaaagaactg gagaatgtat taggatgtaa ccttctgatt cgaacaaggg atggtatcgt
     2941 tttaacagaa gctggaaaga tattctcggt ccattcacgt atgattcttg aagagattga
     3001 aactgcagct cttgaagttg cgaaagtaaa tatagataac acaggaaaag tagcttttgg
     3061 aatttcttcg ctgtttggcg taacaatact gaataatgta cttgataact tccggatgaa
     3121 atatccgaaa aagcgtgtga tgataagaga gggacagttg tcctccctta tccctttcct
     3181 gagagaaggt gttctggatt ttgcaatggg tacattaaca aaagacatgc cagttggaga
     3241 ttttattgtg gttgaattgt ttaacgcacc gttcactata gtggcaagaa aacaccatcc
     3301 tttaaggaac tgtacatcaa tcgatgaact tgcagctgca agatgggtat tcccggaaac
     3361 tcaaatggga tactatcatt acatatcccg tcttattccc tttggacatc cggatgaacc
     3421 atatgctcca atatttaccg attcggtagt ttgtattatg aatcttgttc tctatgatga
     3481 ctatataacg gttctctcca gagcaaggtt acaggaagca agattcaaag aaatattgtg
     3541 ttcaattcct gttaagtatc aactacccgt tagccagtac gggcttattt atccacgaaa
     3601 aaggctaatg acggcaccag cacagataat gattgaatat tttcaacaag tgatacaacg
     3661 atatgactgg aatggatgag caatacccgg aatgatggtg tacaccggga taacaattta
     3721 ttcggatgtg ccaatcccac ggttgtaatg ggcaatataa ttgttagccg attcgctaat
     3781 ggcagggaaa agcacggcat aaatataagc ccacacacat gtaacgtact gcagatactc
     3841 gcttggcaga gttgttactt cttgggaggc agtaccagcc atcagatatt cccgttccat
     3901 gccaaaacct gagttacccg gataccatta cgttgcctgt atttcattca tctgccacac
     3961 acgtgtcagc tctttgtgac ctgacgcaat catctgactc agcagcgatt cacggtactg
     4021 gtctttttgg tgaccgttct gacagagcaa tacagtggga caaacgacca tcgtcatcac
     4081 tggagaggcg tgcatggaaa ctcgtcaaca actcaagttc gccggaacgg aacgttaaca
     4141 atagaacttt actgtataaa tttgaatact gctaaatgta tgaatggttc attcatgtta
     4201 atattgtgat ttgcaaaaat ttcattttat tagagtaaaa tacatgtcta aacaccaaac
     4261 acagctttcc ttactgcagg acgacatccg cagtcgctat gacagtctca gcaaacgtct
     4321 taaacaagtt gctcagtata ttctggataa cagtaatagc gttgcttttg atacggttgc
     4381 ttctattgcg cagcacgcgg aggtacctcc ttcgacatta attcgttttg ccaatgcgtt
     4441 tggttttagc ggattcaatg aaatgaaaca agtatttcgc cagcatttga tggaggaaac
     4501 cgttagctat acagaacggg ctcgtctgtt cagacaaaaa aacatcaatg atggggaacc
     4561 cacaccggaa aaaccgggcg aaattctgaa gctattttcc atggtaaatg cacaggtact
     4621 acaacaatta ccgatgcaga ttaaaaatga gcaactggat gctgcagtaa gtttattgtc
     4681 aaaagccgat aatatttatg tgattggatt acgtcgttca tttagcgtgg cctgttattt
     4741 aacctatgca ttgcgccatc ttgagaggcg tgcattttta attgatggta ttggaggcat
     4801 gttttctgag caactgagta tgatcagctc taacgatgta gtcatcgcca tcagttattc
     4861 gccatacgct caggaggttg ttgaactggt tgagttgggg gcaaaacgtg atgcacatca
     4921 tatcgtcatt accgacagtc aggtcagtcc gttggctgcg ttcagtgagg tctgttttgt
     4981 aatacgtgaa gcgcaggtgg atggcttccg ttcgcaggta gcgtcgatgt gtctggcgca
     5041 gacgttggcc gtatcactgg ctctggctgc tgaataagag gaagatgccg ataatctgtt
     5101 tttcatgatc atcggcattt tggctaagct taatctgaag agcgttcagc ctgaataggt
     5161 tgtggcttgt tcctgcgttt tgtcagcacg atatcctcca ttttctctag tgagacacct
     5221 ttggtttccg gcaggtagcg gcaaataaag aaatagctaa agacacagca ggctgcgaat
     5281 atccacattg gaaatgcacc gtggaaatgg gccagtaacc agggattttc gttaattata
     5341 ggaaagaact gtgaaaccat aaagtttgct atccacataa atcctacaga aatactcatt
     5401 ccctgtgaac gcatgcggtt ggggaatatt tctgatatga gcacccatgc tccaacgccc
     5461 caggaaagtg catagaaaat cataaagaac agcataccaa agagagcata atagccggtt
     5521 gtttggctgt acagagccca tgaagttaat aataaaccaa tgatgctacc aatggtgcct
     5581 tttcgcatta gcgataaacg ccccattttg tccatgatca ttgcgccaat aattgatccg
     5641 acgagttgaa ttacgccaat ccagatggtc tgaaacagcg cttcctgggc gctaccggta
     5701 acatctctca atacaatagg tgcgtaatac atcataacgt taacgccggt tacttgctgt
     5761 aacattgcga tcatgcaacc gaggaaaagg ataaaacgta cgttactgtc atggtaattg
     5821 agcttttggt gggccgtcag ccggtcattt tgcagagagg tttttatatc tgttagcaaa
     5881 tgtcgtgcat gttcctcgtt agatatcttt gtaagaattt ttagcgtttc ttcttcacga
     5941 ccagccatca tcatccagcg gggggattcc ggtataaaga atactaaaat acagaacaga
     6001 atgcatgggg caatgccggc agcaaacata taacgccagc caatctcaat cagccatgta
     6061 ttggtcgcaa tcgaggcgat tttatagttg acgtaaaata tgaggatctg accaaaaacg
     6121 atggcaaact gttgcatact caatgccctg ccacgcatgt ccttaggtga aacctccgac
     6181 atatacattg gagatactgt tgctgcgagg ccgacagcaa gtccacctac gatacgataa
     6241 ataataaagt gcaggaaggt gtaggaaaga gatgttccca ctgctgagat tgtgaacagc
     6301 agggcggaaa tcatgagtga tttcttgcga ccaaaacgtt tggataagta acctgagctg
     6361 aatgatccca ccacacaacc tacaacgacg ctggaaaccg cccagccggt ttcggccgga
     6421 gataggtgaa aataactggt gagagaactg attgcacctg aaataacggc agtatcgtag
     6481 ccaaataata tcccgcctaa tgctgcaata gaacagatac gtaatatata gcctgtattg
     6541 taacgattca ctgatatggg catagctgat tatctcctga gcagaaatca tctatctatt
     6601 tgacctataa tgaaaaaatt gtgtttagtc atttccgtag ctctgcagtt gtacttcttg
     6661 ctttaccatc ggtaattaca tttcaacatt ctgattggta attttcacta agatcaataa
     6721 tgaaacattt attttgttat gtgaatcaca ttttaaatta catgatc
//
LOCUS       DAZGCQ010000195         6742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000195 DAZGCQ010000000
VERSION     DAZGCQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.195"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(74..988)
                     /locus_tag="ACHK07_004917"
     CDS             complement(74..988)
                     /locus_tag="ACHK07_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO8105130.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDERGMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            1204..2637
                     /gene="cscA"
                     /locus_tag="ACHK07_004918"
     CDS             1204..2637
                     /gene="cscA"
                     /locus_tag="ACHK07_004918"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO8105131.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNCKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(2645..3640)
                     /locus_tag="ACHK07_004919"
     CDS             complement(2645..3640)
                     /locus_tag="ACHK07_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8105132.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            3871..4089
                     /locus_tag="ACHK07_004920"
                     /pseudo
     CDS             3871..4089
                     /locus_tag="ACHK07_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            4107..5435
                     /gene="dsdA"
                     /locus_tag="ACHK07_004921"
     CDS             4107..5435
                     /gene="dsdA"
                     /locus_tag="ACHK07_004921"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO8105133.1"
                     /translation="MENAKMNSLIAQYPLVKVLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELIAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMT
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(5543..>6742)
                     /gene="emrY"
                     /locus_tag="ACHK07_004922"
     CDS             complement(5543..>6742)
                     /gene="emrY"
                     /locus_tag="ACHK07_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO8105134.1"
                     /translation="VVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPIC
                     GPILGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVL
                     GVGGLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRN
                     FTIGIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRY
                     GNKIDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTI
                     SFSGLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVF
                     NSSSQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPP
                     FTAKGVG"
BASE COUNT         1831 a         1692 c         1581 g         1638 t
ORIGIN      
        1 ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc
       61 cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg
      121 ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc attcctctct
      181 catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc
      241 ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc
      301 gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact
      361 cttttgccag ggcgcatata tcccgatcgt tctgtgtttt tccactgatt agtcgccatt
      421 cttcttccga gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga
      481 gcaaatgctc gtcttgccat agatcttcac gaatattggg atcgaagctg acaaaacctc
      541 cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca
      601 acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct
      661 ctaaaaaaag atcggcactg gggcgaacca taaacgtaaa tgaacgttcc ccttgatcgt
      721 tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat
      781 cgactccctc agttagcaga gttctttgca ttaacgcacc aaaaggatca tcaccgaccc
      841 gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg
      901 cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct actaccgcat
      961 cccctaaaac ccatactttg gctgacattt ttttccctta aattcatctg acttacgcat
     1021 agtgataaac ctctttttcg caaaatcgtc atagatttac taaaacatgc atattcgatc
     1081 acaaaatgtc atagttaacg ttaacatttg tgatattcat cgcatttatg aaagtaaggg
     1141 actttatttt tataaaagtt aacgttaaca attcaccaaa tttgcttaac caggatgatt
     1201 aaaatgacgc aatctcgatt gcatgcagcg caaaacgcac tagcaaaact tcatgagcac
     1261 cggggtaaca ctttctatcc ccattttcac ctcgcgcctc ctgccgggtg gatgaacgat
     1321 ccaaacggcc tgatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccaatg
     1381 agcgaacact gggggccaat gcactgggga catgccacca gtgacgatat gatccactgg
     1441 cagcatgagc ctattgcgct agcgccagga gacgataatg acaaagacgg gtgtttttca
     1501 ggtagtgctg tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc
     1561 gatggtgcag gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat
     1621 ggtattcatt tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc
     1681 cgcgatccta aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagac
     1741 ccaggcaaca cggggcagat cctgctttat cgcggcagtt cattacgtga atggactttc
     1801 gatcgcgtac tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt
     1861 ttcagccttg gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga
     1921 tacagttacc gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga
     1981 ctttttgcac aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca
     2041 caaagctttg tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa
     2101 tcgccaatgc cctcaaaacg tgaaggctgg gcaggctgca tgacgctggc gcgcgagcta
     2161 tcagagagca attgcaaact cctacaacgc ccggtacacg aagctgagtc gttacgccag
     2221 cagcatcaat ctatctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg
     2281 caagcagttg agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga
     2341 ttacagctcg gcactggaat gcggctgtat attgataacc aatctgagcg acttgttttg
     2401 tggcggtatt acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt
     2461 gacacgctcg ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac
     2521 ggggaagcgg tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt
     2581 tatgcctccc acggagtggc tgtggtgcaa catggagcac tctggcaact gggttaacat
     2641 aatatcaggt ggaacaacgg atcaacagcg ggcaagggat ccgcgtcaca cttccccctt
     2701 cacgaccttc aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa
     2761 ttgtggtcag cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta
     2821 cggcgacatc ctgcgggata cgtaccccct tagccaggag aacctgataa gccacaaagg
     2881 ctgcgcgatc gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtggt
     2941 cattgagtaa acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt
     3001 tcacctcagc cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat
     3061 accctgttgc caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg
     3121 cgagcaaatg ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta
     3181 acgctgggtc atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag
     3241 gcagcgtgat atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa
     3301 gcagctgacg tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat
     3361 tccagccgaa ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag
     3421 tggtagcagt gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca
     3481 tcttacgggc ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt
     3541 cacgcgttgc aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg
     3601 acactcccgc caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa
     3661 aacgggcact gtagcagaaa cagacgtcac tggcgagatc caacgcccta tcacctgaca
     3721 cggcaataca ataaaaaata acaataattc ccggataatt gtacccaatt ccgcctctgt
     3781 tctcgccaac gagtaaccat ataggcaagt aagtaaaaca tgccaactgg catcgccaac
     3841 ctggagaaga aaatataaat atggatcatt gtgctgccgc tgtatcccga catcagcccg
     3901 gaaattattg cgattgctat cggttcaggt gcgattggct gcacgatcgt tacggactcg
     3961 cttttctggc tggtgaagca atattgcggc gctacgctca atgaaacatt taaatactat
     4021 acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt
     4081 atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc
     4141 agtatccgtt ggtaaaggtt ctggttgctc ttaaagaaac cacctggttt aatcctggca
     4201 cgacctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg
     4261 cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttcct gaaactgctg
     4321 ccactggggg gattattgaa tcagaactga ttgccattcc ggctatgcaa aaacggctgg
     4381 aaaaggaata tcaacaaccg atcagcgggc aactgctact gaaaaaagat agccatttgc
     4441 ccatttccgg ctccataaaa gcacgcggcg ggatttatga agtcctggca cacgcagaaa
     4501 aactggctct ggaagcgggg ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc
     4561 cggagtttaa acagttcttt agccaataca gcattgctgt gggctcaacc ggaaatctgg
     4621 ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagtg catatgtctg
     4681 ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat
     4741 atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga
     4801 actgtttctt tattgatgac gaaaattccc gcacgttgtt ccttgggtat tccgtcgctg
     4861 gccagcgtct taaagcgcaa tttgcccagc aaggccgtat cgtcgatgct gataaccctc
     4921 tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta
     4981 aactggcgtt tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta
     5041 tgttgttagg cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg
     5101 acaaccttac cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg
     5161 caatggagcg tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc
     5221 ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc ttcggcactg gcgggtatga
     5281 ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag
     5341 aacaactgcg taataccact catctggtgt gggcgacggg aggtggaatg gtgccggaag
     5401 aagagatgaa tcaatatctg gcaaaaggcc gttaataacg tttcaacgca gcatcgccat
     5461 cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatccccg gtcttacccg
     5521 ctataacccc ctgcttttac catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa
     5581 accaaaccaa aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg
     5641 cagaaataga aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa
     5701 gcgaaccata gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat
     5761 cgatggttgc tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg
     5821 tcaacgacgt accaacggat cctgacaagg tacgaaaaaa attactcata ctcgaggcat
     5881 tggcaaattt attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac
     5941 aggcaacggc gaatccctga aaaaactgcg gcaaaatgat acctgtaaaa tcaatcgttg
     6001 gcataaatgt cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctaaatgtca
     6061 ccaacacccg catgtcaatt ttgttgccat aacgtcctat caaaggcgaa atcaatagtg
     6121 gcatgatgcc gatgggcgca taagcaagtc cggcccatat cgcattatac cccatcgttt
     6181 cctggagtaa ctgcggcata aggactatcg ctccagagta aaataaatac gcgcaggtga
     6241 tactcacaat accaatggtg aaattacggg acttaaacaa actgaggtca agaatcgggt
     6301 tctctgaggt cgactcccaa atgactaaag agatcagaga aataactgat actactgtta
     6361 atattattat tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt
     6421 gcaagccacc aacaccgagc actaacaggg tcagtcctgg tagattcatt ttgaccggtg
     6481 aagtctcagt ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca
     6541 tagggacatt gattaaaaat atccaacccc agctaaagtt atcgcaaata tatccgccta
     6601 atatcggccc acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg
     6661 ttcttttttc tggtggataa ttccttaata ataaactctg tgacagtgga attaacggcc
     6721 ctgccattaa cccctgaacg ac
//
LOCUS       DAZGCQ010000196         6673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000196 DAZGCQ010000000
VERSION     DAZGCQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.196"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            271..1122
                     /locus_tag="ACHK07_004923"
     CDS             271..1122
                     /locus_tag="ACHK07_004923"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8105135.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1149..2138
                     /locus_tag="ACHK07_004924"
     CDS             1149..2138
                     /locus_tag="ACHK07_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8105136.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2169..3062)
                     /locus_tag="ACHK07_004925"
     CDS             complement(2169..3062)
                     /locus_tag="ACHK07_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8105137.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINIRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYITSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3263..4189)
                     /locus_tag="ACHK07_004926"
     CDS             complement(3263..4189)
                     /locus_tag="ACHK07_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8105138.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4346..5266
                     /locus_tag="ACHK07_004927"
     CDS             4346..5266
                     /locus_tag="ACHK07_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8105139.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            5501..6643
                     /locus_tag="ACHK07_004928"
     CDS             5501..6643
                     /locus_tag="ACHK07_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO8105140.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT         1887 a         1577 c         1496 g         1713 t
ORIGIN      
        1 gagtgaataa taatgaccta cctttaatta gcaactattg gaaatacgtc gaagtaggta
       61 tatacgccag gataaaccga tatatttttt atggatattc attttccttc atcggactgt
      121 aaattgcgga gatatcgaac ttgcactacc catcgcaaca ctcaaacaga tttatgaatt
      181 tcgttcataa cccctgacaa atcctgccgt ctaatcgagc ttttcccttt acgttatgct
      241 tctcatgaac gagaacggaa ggataacacc atgcaaaaag taaaactgaa caacggtatt
      301 gaaatgcccc tgttgggctt tggtgtattt cagatgaccg atgccgctga atgcgaaaga
      361 gcggtcattg acgctattaa caccggttat cgactgattg acaccgccgc ctcctatcaa
      421 aatgaaaccc aggtcgggaa cgcactgaaa caaagcggta tcgcccgtaa cgaactcttt
      481 gtcaccacga aactctggtt gcaggatacc agttacgaag gggccaaagc ccagttcgaa
      541 cgctctctga atcgactgca acttgattac gttgacctgt atttgattca tcagccttac
      601 ggtgatgtcc acggtgcctg gcgtgccatg gaggagctgc aacaggccgg taaaattcgc
      661 gctattggcg tcagtaactt ccatcctgac cgactggctg accttatcgc ctttaataac
      721 gtcgttccag cggtaaacca ggttgaagtt aaccctttca atcagcagct acacgccgtt
      781 ccgtggatgc aaagccgtgg tattcagcca gaagcctggg caccgtttgc ggaagggaaa
      841 aatggtctgt tccagcatcc tgttctcacg gcaattggcg agaagtatgg caaaagcgta
      901 ggccaggttg ttctgcgctg gatctaccaa cgaggcattg tttcgcttgc aaaatcagta
      961 cgcaaagaac gcatggaaga gaacatcaac attctcgatt ttgaactcag tcctgaagat
     1021 atgctgcaaa ttaccgccct ggataccgca accagcgcgt tcttctctca ccgcgatccc
     1081 gcaatggtgg aatggctgac tggccgcaaa cttgatgttt aagccgcgat aaaagaggta
     1141 ttcattcaat gcaaaaacgt tatctgggta aatccggact cgaagtttcc gcccttgggc
     1201 tcggttgcat gggcttaagc cacggctacg gcccggcgac cgatacccgt caggctatcg
     1261 aacttattcg tgctgcggtt gaacgtggcg tcaccttctt cgataccgcc gaagtgtatg
     1321 gtccgtatct gaatgaagaa gtggtcggcg aagccttaaa accgtttcgt gaccgcgtgg
     1381 tgatcgccac taaattcggt tttaccttcg gcgatgataa taagcagcag attttaaaca
     1441 gccgtccgga acatatccgt gaagccgttg aaggatcatt acgtcgtctg aagacagatg
     1501 ttattgacct gctgtaccaa caccgtgttg atccggatgt tcctattgaa gatgttgcag
     1561 gaacggtgaa agacctcatc gcagaaggta aggtcaaaca tttcggtctg tccgaggctg
     1621 gcgcacaaac cattcgtcgt gcgcatgcgg tacaacctgt caccgccctg caaagcgaat
     1681 attccatgtg gtggcgtgag cctgaacagg aaattctgcc gttgctggag gaactgggga
     1741 ttggttttgt ccccttcagc ccactaggta aaggtttcct gaccgggtcg attaagccgg
     1801 gaaccacttt cggcaaagat gattaccgca gcaccgtacc gcgtttcgca gcacaagcca
     1861 ttgaagctaa tgaaaaactg gtcaccttgt taggtgaact ggctgcggag aaaggggtaa
     1921 cgtctgcaca aatcgcactg gcatggctgt tagcacaaaa gccgtggatt gttcccatcc
     1981 ctggcaccac taaactgcac cggctggaag aaaacctggc cgctgctgac attgttcttt
     2041 cgcagaaaga tacgcagcag ataaccgagg cgctggaaac aattaaaatc gtcggtgaac
     2101 gttactcgcc tgaacaccag gcacgcgtag gccgctaata cccacacggg tcctggacaa
     2161 aggacccgtt attcggtgta gcgaagcgca tcgatcaaca gcgcaaaagc aggcggatgc
     2221 tgcttgcggc tggggtagta gagataatat ccgggaaaag ttggacacca ctcctgcaac
     2281 acctgaataa gcttgcctga cgttatatac tcctgaacca tgtcttcagg gacacaagcg
     2341 atcccaaacc ctgataacgc ggcatcaatt ctttctggca atggactaca cgtcagctgc
     2401 ccatcaacct taacgcgaag cgctttccct tctcgctcga attcccagtg gtaaagtcca
     2461 ccggcagttg gcaaacgtat gttgatgcag cgatggtttt gtagctcgtg aggcgtttca
     2521 ggcgcagggt ttgcagcaaa atacgccggt gcccccacca cagccatccg catatctggt
     2581 ccaattctga ccgcaaccat atctttatca acattttcac ccagtcgtat cccggcatca
     2641 aaacggcctt caacaatatc gacaaaaccg ttatccacca ccagttcgac actgatttcg
     2701 ggatactttc tgagaaaagg ttttagcttc ggccagacca gacttcgcgc ggcatgttcc
     2761 ccggcagata aacgaatatt cccggagggc gtgccgttct gttgaataag tgactccagt
     2821 tcctgttcaa gctcgtcaat acgcggttca aggcaggcaa tgattctctc ccccgcttct
     2881 gtgggagcca cactgcgggt agtccgtgtc aggaggcgga tattgagcct ttcctccaga
     2941 gccttcattg catggctaag tgcagactga gagacgccga gtttgcccgc tgctttggta
     3001 aaacttcgct ctcttgccac cacaagaaag atttgcagtt cgttgaagtt ttctttgagc
     3061 attggcgatt tccttgtaga aacatcctct tcacagacac tcatgaaggg ggggcataga
     3121 tgctattatt ttagccacat tattagtaac aatgataatc tggctgtatc gaaaataatt
     3181 ctaaactttc agaagattta ttatgaataa acgtatcctc acgattgtgc cattcttctt
     3241 accgatattc tgacacatat catcaatttt cgagttttac tcttttctcc accataaaat
     3301 caataaaagc acgaacttta gagggcattc tcacccgtga tgaaacataa atataaaatc
     3361 caggaaatga aacagcccaa tcctcaagaa tcggctgtaa cttgcccgca ttgatatgtt
     3421 cagcgattgc aaaatccaga tgttgaatga tacccgttcc attcagcgcc attcgtatca
     3481 tgctttcgtc atcattagaa atatacttac ctgaaacctc tatttcgaac tcttcattac
     3541 cccccacaac attagaaaac tcccatgcag agagtgcacc gctactttta tgccgcaacc
     3601 gaaggcaatt atggttactc agttcctgag gcgtttcagg aataccgtgt tcttttaaat
     3661 aatctggcga tgccacagta accagtttaa taagcggaga aacaggaacg gcagtcatat
     3721 gctcatccag tccttgctca agacgtatgc ccacatcaca gccgtccgca ataatgttag
     3781 ataaaccgtc atccataatg agttctatct ttgttttggg gtaacgagta agaaactcgc
     3841 caatatgagg ctcaacaagc atccgtgctg caacacggga ggaatttatt ctcaacaggc
     3901 ctgtaggttc aagttgggta tcaccaacat ctttcaaagc atcatcaatt tgccccaaag
     3961 ccgacaccag cacttcatac agacgctgtc cttcctcagt aagcgacata ttgcgggtcg
     4021 tacggtatat cagcttggtg ttaagacgtt cctcaagaga ttttaaattc tgggacaatg
     4081 cggcccgcgt aatacccatt tcagaagcgg ctctggaaaa actgccggca cgaacaatca
     4141 gcgcaaacca ggcaagagaa ggcaaaattg aaggctctat tttcatcatt gtaaacctta
     4201 acttaacact gtgatagata ttagaccatt gatgataacc aatacaagtc atactattac
     4261 gccgttctcg atatgaacac gaatgccagt tgcggataat agaaatgata tttaaatctg
     4321 tttaggcaaa tccccggagc ttgaaatgaa aactgtttcg ataaaacaca cttactggga
     4381 tatagctgct gatatctatt tccctgacga ttttaatagt gaaaaaaagt atcctgccat
     4441 tatcagcgcc catccaattg gaagctgcaa agaacagaca tcgggtagtg tgtatggtgc
     4501 cgccctggca aaagctggct ttatcgtcat tgcttttgat gccagttttc agggaagcag
     4561 cggtggggaa ccgcgttatc tggaagatcc aacaatgcga gtaaaagact tcagcattgt
     4621 cgttgattac cttaccagct taccttacgt tgatgccggg cgtattggtg tactgggtat
     4681 ttgtggtgga ggtggatatg ccattaatgc cgcaatgaca gaacgtcgaa ttaaagctat
     4741 tggtactgtc acgggtgcaa actacgggcg tcttatgcgt gagggattta cagcgtttaa
     4801 tcctatcgga gcactcgaag caatggcgca acaaagaaca gacgaagcta acggcgcaaa
     4861 acttcgcgta gatgatttac tcccctcttc tccggatgca gctcttgaag cgggattaac
     4921 agaaatagac ctttttgaag ccacagaata ttatcgttcg ccacgaggct atgcacctaa
     4981 tggggtaaat cgttcgctat tctcacatca gactgttgcc gtgggttggg atgcatttca
     5041 tcttgcagaa gtcctgttaa ctcaaccaat aatggttgtg gtgggcgata gagtgggcgc
     5101 atttggtgcc taccgtgacg gctgcgaaat tattggccgc gctgcttcaa aacataaaga
     5161 gctggtcgtg gttgaaggtt attcacatta cgacctgtat gataagccag aacctgtgaa
     5221 acaggcactc gataaattaa ttccttttta taaaacacat ttgtaataat cttacaggga
     5281 gattccttat ctccctgaca tttatccttt attgactaca caacaataat aaaattactc
     5341 aaaaagaaat ttcattaaaa aacaaaaaag cagcaactca attattagaa accctttaaa
     5401 gggattataa ataaagccag aactcttggt tccaaagctg gcaacaacta accagttaaa
     5461 acatacgcat caaaatcaga taaacatttg aggtattgtt atgacaaata aacacccatc
     5521 attgttttct ccttttatgc tgacagaaaa aataaagcta cgtaatcgta tcgttatggc
     5581 tcctatgacg acctggtcag cgaatcctga tggaaccatc tctgagcaag aactcgaatt
     5641 ttataaacgt cgctcacaaa atgttggttt agtaataaca ggatgtacct atgtcacgcc
     5701 aagcggcatt gggtttactc atgaatttgc cgcgtatgac gaccgtttca ttaacagcct
     5761 tgaaaagtta gcagcagcag ccaaaagtgg gggtgctccg gctattttgc agattttcca
     5821 cgccggtaat aaagccatcc cggagcttgt accaaataac gatgttatta gtgccagcgc
     5881 atcaagcgta aaatctggtg actttatgaa gagagtggta caaagcaggg aaatgactga
     5941 aaacgaaatt caggaaacca ttagagcatt cggagatgtc acaaaaaggg cgattaaagc
     6001 aggttttgat ggcattgaac ttcatggtgc gcacggattc cttctacaga atttcttctc
     6061 ccctctattt aaccagcgta atgaccgatg gggaggagat ctcgaaggac gtatgcgctt
     6121 cccgctggcc gtattacagg aagtaaaaaa tgtagtctat gaatatgcga caaagccctt
     6181 tgccatcggt tacagaatat ctcctgaaga atctgcaaca ggggggctgc ggatagagga
     6241 tacttataaa ctccttgatc ggctcatttc atccggtatt tcgtatatac atacctcgct
     6301 ggtcagtatt aacgatagct accctgttga gtcgccaaat ggcccccgaa ctattgaatt
     6361 gattttaaat catattgcag gcagagtgcc ggtaatcgct gcaggtaaaa tacgcacacc
     6421 gtctcaggct caagaagcaa tttccactgg cctgccactt gttgcgatcg gtaaagggct
     6481 ggtgataaat ccagaatggg tgactttagc tgaatcaggt agaggccatg aaattcaaac
     6541 aacccttaat ccacaacttg ttcctgaatt aaccattccg gataaactat gggatcaaat
     6601 tcaggcatcg aaaggaactg gctggtttcc gttaatggat taaatatcta ttactctccc
     6661 gagagtaaaa cta
//
LOCUS       DAZGCQ010000197         6577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000197 DAZGCQ010000000
VERSION     DAZGCQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.197"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..2237)
                     /gene="betT"
                     /locus_tag="ACHK07_004929"
     CDS             complement(204..2237)
                     /gene="betT"
                     /locus_tag="ACHK07_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO8105141.1"
                     /translation="MTDLSHSRENDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2366..2953
                     /gene="betI"
                     /locus_tag="ACHK07_004930"
     CDS             2366..2953
                     /gene="betI"
                     /locus_tag="ACHK07_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO8105142.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2967..4439
                     /gene="betB"
                     /locus_tag="ACHK07_004931"
     CDS             2967..4439
                     /gene="betB"
                     /locus_tag="ACHK07_004931"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO8105143.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4453..6141
                     /gene="betA"
                     /locus_tag="ACHK07_004932"
     CDS             4453..6141
                     /gene="betA"
                     /locus_tag="ACHK07_004932"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO8105144.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDLN"
BASE COUNT         1642 a         1794 c         1774 g         1367 t
ORIGIN      
        1 aaatatataa ccctatagga ctgaatccat tcagctaata caaaataaaa tcgttaatta
       61 ataaaatata aaatcatgaa gtgatacaag aggaatttca ggatatgaca tgacaatcat
      121 atataatata tgcataattt tttacagcga gtcaatgatt cccgcgccat atgcagacat
      181 ggcgcggttt tatgcaataa caatcacgcg tccgggaaca tcacgctatg gcccggcgct
      241 tcacgatgga gatgaataaa gttaaggtgc cgctcgtact ggtcaagaat atcggtgatc
      301 acctgctctt tgctgtagtc catcaggtcg ttgccctggc tgccttctaa caggaaggtt
      361 tccagccggt agtaggtcga tttcccgcta cgtgcgcggt aggtaaagcc cggcaccgag
      421 tattgctgcg gccaaatctg atagacaaag ttttgctctt cgcccatatg caccaacaaa
      481 tccagatgcc ccaactgttg tccctcttcc ggcggtaggc tttttagctc cacatacgcg
      541 ccgcgcaacc gcagctcttg cgccacttct tccattgccg ggtaacagac cgtctccatc
      601 atctgtttag tgtaacgcgt gcccggataa ttcatcaggc gcgagagacg ttttttccag
      661 ctcaggcgat cctgaagccc cagcggtcgc ggtgcggtat cgcggttggc actttcacgg
      721 cggtaatctt ctaccttcag agatttatac aaccccgcca tcacgaagaa gatcacaaag
      781 ctgaacggca gccccataat caccgtggtg ttttgcagcg cggatatccc gttggtcatc
      841 agcatgccga gcgtcagcag gccaatcgcc accgaccaga agacgcgcag ccagccgggg
      901 gcgtcgctgt tgatatcttt aagctgcgag gtgaaattcc ccagcaccag cgccccggag
      961 tccgccgagg tcacataaaa cagcaggcca gtaatggtgg cgacggaggc gctaaaggta
     1021 aacgccggat actgcgccag caggctgtag aagccgcgct ccggatggac catcgcttcc
     1081 tctgcaaatg ccgcgccgcc gtggatgatt tcatacagcg cgctattgcc gaacaccgag
     1141 agccataaca gcgtgaaggt aaacggaata atcaacgtgc ccagcacgaa ctggcgaatg
     1201 gtacgcccac gcgagatacg cgccaggaac aagccgacaa acggcgacca tgccacccac
     1261 catgcccaga agaagagcgt ccagttattc atccactcaa ctggacggtc gaaggcaaaa
     1321 ctgttgagcg tcatgcccat aaagcgattc acatagtcgc caacattcag caccagtgca
     1381 ttaagcagga acgaagtgtc gcccataaac aacacgaaca ggatcaatcc cagcgccagc
     1441 gcgacattaa gctccgataa cacgcgaatg cccttatcga caccggatgt caccgagata
     1501 gtggcgatta tcaccgacaa ggcgatcagt gccgcttttg ccgccatcga atcgggaata
     1561 tcaaacagta cgctcaagcc atagttaagc tgcaccacac cgataccgag cgtagtggca
     1621 ataccgaaga tagtgccgat caccgctgca atatccacag agtgacctat cggcccgtta
     1681 atccgtttac cgaagatcgg gtacagcgcc gagcggatgg tgagcggcaa attataacga
     1741 tagctaaagt atccgagcgc catgcccatc agcgcataca tcgaccagcc ggttaagccg
     1801 tagtgaaaca gcgtccagac catcgcctga cgcgcggcct caatcgtctg tcccgcacct
     1861 tccggcggct gcatatactg cgttaccggt tcggctacgg aaaagaacat cagatcgata
     1921 ccgatcccgg cagcaaacag catcgccgcc caactcagca ggctgaattc cggtttggat
     1981 tgttctggcc cgagcttcac cgaaccaaaa cgcgaacaag cgatacagac cacaaagaca
     2041 atatagagcg ttgccgccag cagatagtac caaccaaagg ttttagaaac ccagtccagc
     2101 gtgcggccaa tccacagggc cgagaagtcg cgaaacagga tcgttgtcag ggaaaacaac
     2161 aaaatcagtc cggcggaggt gtaaaacacc accggattga ttttgtcatt ttccctgctg
     2221 tgtgaaaggt ctgtcatcca gtatccccac tgttattgtt accatttaaa atcaaattcg
     2281 taacaattaa gacacatttt atattgaacg tccaatcaat aaccgcttta atagataaac
     2341 aacgctgatg aatggagtgg cgaaaatgcc caaattgggg atgcagtcga tccggcgcag
     2401 acaactgatc gacgccacac tggaagcaat aaatgaagtg ggcatgcacg atgcaacgat
     2461 cgcgcagatc gcccgccgtg caggcgtttc tacggggatc atcagccact atttcaggga
     2521 caaaaatggt ctgctggaag caaccatgcg cgatatcacc agtcagctac gtgacgcggt
     2581 tttgaatcga ttacatgcac ttccgcaggg cagtgcagag cagcgattac aggcgattgt
     2641 tggcggaaac ttcgatgaaa cgcaggtgag cagtgcggcg atgaaagcct ggctggcgtt
     2701 ctgggccagc agtatgcatc agccgatgct ctatcgttta cagcaggtca gcagccgccg
     2761 cttgctgtcg aatctggtga gcgagtttcg ccgcgaattg ccgcgccacc aggcacagga
     2821 agcgggctac ggccttgccg cgctgattga cggattatgg ctgcgcgcgg cactgagcgg
     2881 caaaccgctg gataaacctc tcgcacactc gctgacccgt cactttatta ctcagcattt
     2941 acccaccgat taaccgagga gacgtgatgt cccgaatggc agaacagcag ctttatatac
     3001 atggtggtta tacctccgcc accagcggtc gcactttcga gaccattaac ccggccaacg
     3061 gtaacgtgct ggcgaccgtg caggccgccg ggcgtgagga tgtcgatcgc gccgtgaaga
     3121 gtgcccaaca ggggcaaaaa atctgggcgg cgatgaccgc tatggaacgc tcgcgtattc
     3181 tgcgtcgggc cgttgatatt ctgcgtgaac gcaatgacga actcgcaaaa ctggaaaccc
     3241 tcgacaccgg aaaagcatac tcggaaacct caaccgtcga tatcgttacc ggtgcggacg
     3301 tgctggagta ctacgccggg ctgatcccgg cgctggaagg cagccagatc ccgttgcgtg
     3361 agacgtcatt tgtttatacc cgccgcgaac cgctgggcgt ggtggcgggg attggcgcat
     3421 ggaactaccc gatccagatt gccctgtgga aatccgcccc ggcgctggcg gcaggcaacg
     3481 caatgatttt caaaccgagc gaagtcaccc cgcttaccgc gttaaagctg gctgaaattt
     3541 acagcgaagc gggcctgccg gacggcgtat ttaacgtgtt gccgggcgtg ggcgcggaga
     3601 ccgggcaata tctgaccgag catccgggca ttgccaaagt gtcatttacc ggcggtgtcg
     3661 ccagcggcaa aaaagtgatg gctaactcgg cggcctcttc cctgaaagaa gtgaccatgg
     3721 aactgggcgg taaatcaccg ctgatcgttt tcgatgatgc ggatctcgat ctcgccgccg
     3781 atatcgccat gatggcaaac ttcttcagct ccggtcaggt gtgtaccaat ggcacccgcg
     3841 tcttcgttcc ggcgaaatgc aaagccgcat ttgaacaaaa gattctggcg cgcgttgagc
     3901 gcattcgcgc gggcgacgtt ttcgatccgc aaactaactt cggcccgctg gtcagcttcc
     3961 cgcatcgcga taacgtgctg cgctatatcg ccaaaggcaa agaggaaggc gcgcgcgtac
     4021 tgtgcggcgg cgatgtactg aaaggcgatg gcctcgataa cggcgcatgg gtcgcgccga
     4081 ctgtgttcac cgattgcagc gacgatatga ccatcgtgcg tgaagagatc ttcgggccag
     4141 tgatgtccat tctgacctac gagtcggaag acgaagtcat tcgccgcgct aacgataccg
     4201 actacggcct ggcggcggga atcgtgacgg cggacctgaa ccgcgcgcat cgcgtcattc
     4261 atcagctgga agcgggtatt tgctggatca acacctgggg cgaatccccg gtagagatgc
     4321 ccgttggcgg ctacaaacac tccggcattg gtcgcgagaa cggcgtgatg acgctccaga
     4381 gttacaccca ggtgaagtcc atccaggttg agatggctaa attccagtcc atattctaac
     4441 caggaggttt atttgcaatt tgactacatc attattggtg ccggctcagc cggcaacgtt
     4501 ctcgctaccc gtctgactga agatccgaat acctccgtgc tgctgcttga agcgggcggc
     4561 ccggactatc gctttgactt ccgcacccag atgcccgctg ccctggcatt cccgctacag
     4621 ggtaaacgct acaactgggc ttacgagacg gaacctgaac cgtttatgaa taaccgtcgc
     4681 atggagtgcg gacgcggtaa aggcctgggt ggatcgtcgc tgatcaacgg catgtgctac
     4741 atccgtggca acgcgatgga tctcgacaac tgggcaaaag aacccggtct ggagaactgg
     4801 agttatctcg attgcctgcc ctactaccgc aaggccgaga cccgcgacgt gggcgagaac
     4861 gactaccacg gcggcgacgg cccggtgagc gtcaccacct ccaaacccgg cgtcaatccg
     4921 ctgtttgaag cgatgattga agcgggcgtg caggcgggct acccgcgcac ggacgatctc
     4981 aacggctatc agcaggaagg ttttggcccg atggatcgca ccgtgacgcc gcagggccgt
     5041 cgcgccagca ccgcgcgcgg ctatctcgat caggccaaat cgcgcccgaa cctgaccatt
     5101 cgtactcacg ctatgaccga tcacatcatt tttgacggca aacgcgcggt aggcgtcgag
     5161 tggctggaag gcgatagcac cattccaacc cgcgcgacag cgaataaaga ggtgctgtta
     5221 tgtgcaggtg cgatcgcctc tccgcagatc ctgcaacgtt ccggcgtggg tagcactgaa
     5281 ctgctggcgg agtttgatat tccgctggtg catgatttac ccggcgtcgg cgaaaatctt
     5341 caggatcacc tggagatgta tctgcaatat gagtgcaaag aaccggtttc cctctaccct
     5401 gccctgcagt ggtggaatca gccgaaaatc ggtgcggagt ggctgtttgg cggcaccggc
     5461 gttggtgcca gcaaccactt tgaagcaggc ggatttattc gcagccgaga ggaatttgcg
     5521 tggccgaata ttcagtacca tttcctgcca gtagcgatta actataacgg ctcgaatgca
     5581 gtgaaagagc acggcttcca gtgccacgtc ggatcgatgc gctcgccaag ccgtgggcat
     5641 gtgcggatta aatcccgcga cccgcaccag catccggcga ttctgtttaa ctacatgtcg
     5701 cacgagcagg actggcagga gttccgcgac gcaattcgca tcacccgcga gatcatgcat
     5761 caaccggcgc tggatcagta tcgtggccgc gaaatcagcc ccggcacgga atgtcagacg
     5821 gatgagcagc tcgatgagtt cgtgcgtaat cacgccgaaa ccgccttcca cccgtgcggt
     5881 acctgcaaaa tgggttatga cgagatggca gtggttgacg gcgaaggccg cgtgcatggg
     5941 ctggaaggcc tgcgcgtggt ggatgcgtca attatgccgc agattatcac cggcaatttg
     6001 aacgccacga cgattatgat tggcgagaaa atagcggata tgattcgcgg gaaggatgca
     6061 ttgccgagga gcacggcggg atattatgtg gcaaatggga tgccggtgag agcgaaaaaa
     6121 atgagtcgtg atttgaactg actcaggaac cgatcagatt gagagttacc gttccaggta
     6181 tggggacaga atcgttatat aaaacatagg ggtaactctc atgcttcata gtgacaacct
     6241 cgccagcaaa ttctaagctg acttgctcaa tctggctgat gaaggtgcta tgcatacaca
     6301 gataaactac agatgaaata ccactcagct taagaattac gccgctaatg ttccttgcta
     6361 tggttacctg ttcattcccg ctcacggtta gcatgtacga aaagggttct tgtaacgagt
     6421 acaaaaataa gtcagaccaa aaccacatgg agctttaaac acggttcgta taatttatta
     6481 gcagcgattt atttttactc ttctttaatt aatcatcatg cttaaatatt ttttagcaca
     6541 agattatcgc ttcgctaaca ttttatttta cattgat
//
LOCUS       DAZGCQ010000198         6458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000198 DAZGCQ010000000
VERSION     DAZGCQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.198"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1440
                     /locus_tag="ACHK07_004933"
     CDS             <1..1440
                     /locus_tag="ACHK07_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8105145.1"
                     /translation="LSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQL
                     ASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARERELTGELLE
                     SRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDEQT
                     ELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSGVGKTETA
                     LALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSV
                     VLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQLLDE
                     QPEATEASLHELLRPILRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLAQVCERLH
                     CHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKPQ
                     ALTLAWSDEDGIVLVFDAQAEGESSCAVS"
     gene            1425..3782
                     /locus_tag="ACHK07_004934"
     CDS             1425..3782
                     /locus_tag="ACHK07_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8105146.1"
                     /translation="MRSFLTSKADSVVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYRVSGHSSSPYLTPGQVLETDG
                     TLPDVVREGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVISGSLPARI
                     ESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGFHSPLIDG
                     TEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRMEDKRQEEH
                     IKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADAQARAQGA
                     VLEMAAAISQINQANSQMQALNSSAEAAGALLSDINTQIRFVTDKIRELQSAVLLGSA
                     PQGVALTSGEHLQLCSTRNTMINAGQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVAN
                     QGDIEIQAQHNTMALFSEKQLTVTSSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGS
                     SGEFIVKTSDYLVPGSGVDLPNEAPGFSLTDITQESKISSKSFND"
     gene            3798..6026
                     /locus_tag="ACHK07_004935"
     CDS             3798..6026
                     /locus_tag="ACHK07_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO8105147.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYEAEAENQWVVQD
                     TLRAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKRKYGLVTL
                     KGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEVMVFDKTVSLSS
                     PFAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDVFTETFTKDTRVTARTGILPLKQVQTEVDKSTKQEYWQLRPENAY
                     VPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDYLDGKKQTTGFFRSLINSLYEAANDD
                     TRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFK
                     KDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPSLWHMHPIMFLASL
                     IQPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTDFSKHPNIRHEANGVVS
                     TAAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRKALDVVMQGKISEAIHL
                     CRIEWASLPGSPHGQPTANKKMIMEKYELYLAEEKEGKTSLHATTEKIVKFIEGNYPE
                     YL"
     gene            6023..>6458
                     /locus_tag="ACHK07_004936"
     CDS             6023..>6458
                     /locus_tag="ACHK07_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105148.1"
                     /translation="MKKFIFLLLIPVFCNANTPTVFKSSCSGIQYSIESFCSVNNLND
                     DQDKLTIPICSKQIFTLGKENHDLSESTGFVERINIKGEKIKMMNFVFYGATCKSDRI
                     ILSGAGGCNSCGEMFKIFNLSGKEINEKSTMLDLEAMQDIIPD"
BASE COUNT         1675 a         1523 c         1774 g         1486 t
ORIGIN      
        1 ctgtcgcgcc gttatctgac agaacgtcag ttaccggaca aggccgttga cctgctcgat
       61 actgctgccg cccgtgtgcg tatgagcctc gacacggtgc cggaacagct gacccgtatc
      121 cgttcacagc tcgcttccct cggtatggaa aaacaggcac tgctggaaga tattgccgtt
      181 ggtcatcaga atcacggcga acgtctgtct gccattgagc aggaagagaa cgtgctgatg
      241 acagcacgtg atgacctgga gcagcagtac gcccgggagc gcgaactcac cggtgagtta
      301 cttgaaagtc gcagcgatat ttcccgtcag agtgagacac atcacctgca gcaggcactg
      361 cacgacattc agcagaatca gcccctgctc agtgtggatg ttgacgtacg cactgttgcc
      421 ggtgtggtcg cggaccggac cggggtgccg ttatcttccc tgatgaagga cgaacagacg
      481 gaactgttgc atctggaaaa ggatatcggc agacgggtgg tcggacagga cgtggcactg
      541 gagtccattg cgcagcgtct gcgtgcggcg aaaacgggtc tgacatccgg taacggcccc
      601 cagggggtgt tcctgctggt tggtcccagt ggtgtgggaa aaaccgaaac ggcactggcg
      661 ctggcagacg tgatgtacgg cggcgaaaaa tcactgatta ccatcaatct gtcggaatac
      721 caggagcccc atacggtttc ccagcttaag ggttcaccgc ccgggtacgt gggatacggt
      781 cagggcggta ttctgacgga ggctgtgcgt aaacgccctt acagcgtggt gctgctggat
      841 gaagtggaaa aggcccaccg ggatgtgctg aatctgttct accaggtgtt tgaccggggc
      901 tttatgcgcg acggcgaggg gcgtgaaatt gacttccgta atacggtcat tctgatgacc
      961 tccaacctgg gcagtgacct tctgatgcag ttgctggacg aacaaccgga agccactgag
     1021 gccagcctgc acgaactgct gcgcccgata ctgcgcgacc acttccagcc tgcactgctc
     1081 gcccgtttcc ggaccgtgat ttaccgtccg ctgacgcagt ctgccatgcg caccattgtg
     1141 gagatgaagc ttgcacaggt ctgtgaacgc ctgcactgcc attatgggct gagcacatcg
     1201 gttgatgaac gtgtgtacga tgccctgaca tccgcctgcc tgctgccgga tacgggtgcc
     1261 cggaatgtgg agagtctgct gaaccagcag ctgctgccgg tactgagccg gcagttgctg
     1321 agccatatgg ccgcgaagca gaaaccgcag gcgctgactc tggcatggag tgacgaggat
     1381 ggcattgttc tggtatttga tgcacaggca gagggagagt cgtcatgcgc agtttcctga
     1441 ccagcaaggc tgacagcgtt gtacagcagc tttcagggct atcacggtac cgggttcatg
     1501 tgcatgaatg cagccatttt ctggatgtgt tgcgttacag cgcggttgag tccctcagcc
     1561 agccctggcg ctatgacgtg gttgtcacct gctcttctgc ggatattgcc tgtgacgcgt
     1621 tactgctgaa gccggcgtca tttactttcc agaccccggt gtctgacgga acaccggccc
     1681 tgccgctcag aacggtattc ggcgtggttg agtcgtttcg tcgcatttct gcctcgaatg
     1741 atgagacccg ttacgcactg acgatagttc cccgtattgc ccttctgaac tatacgaaag
     1801 ggagcgaaat ctacctcaat cagtcagtca ctgaagtggt tgagcaggta ttgcgtaaac
     1861 atggtctgga agggcaggat tttgagttcc ggctctcccg ggagtatcca tcccgggagc
     1921 tcatcacccg gtggcgggaa actgaccttg agtttatccg gcgcctgctg gcggaggtgg
     1981 ggatttactg gcgttatgaa atggacagcc gtcttgagca ggacatagtg attttccagg
     2041 acagccagca gcagtatgaa tctggcatca cgctgccatt gcgcaatcag gccgggatga
     2101 gtgacagcgg acaggagagc atctgggata ttcagaccac ctgtaacgtg gtgagcggaa
     2161 gtgtggtcac ccgggactac aactaccgtg aagcgctgaa acctcaggac agtgccgaaa
     2221 gtattttcag taaggaaggg atcaccaccg gagaaacgta ccattatgct gaacccttcc
     2281 tgacggcagg tgacgctgaa agcccggaga ccggggcata ctttgcccgt ctgcgtcacg
     2341 aacgtattct gaatgcgcag tatcgtgtca gcgggcattc ttccagccct tatctgacgc
     2401 cgggtcaggt acttgaaacg gacggcacgt tacccgatgt cgtcagggaa ggcatcgtta
     2461 tcaccacggt gcgttcccgc ggttcacgca aaagtcgctt tacgctgacg tttgagggga
     2521 tcccctacag tgaaacggtc tgctggcgtc cggcactgct cagtcgcccg gtgatatccg
     2581 gctcgcttcc ggcgcggatt gagagtcacg agaagaacga tatctacgct catctggacg
     2641 atcaggggcg ttaccgggtg aagctggact ttgaccgcaa cagtacagaa cggggttacg
     2701 cttatctgtg gttgcgtctg gctaaaccct gtgccggtga tacgtacggt ttccactcac
     2761 cgcttatcga cggaacggaa gtggccattg tatttgacgg cggagatccg gatcgtccct
     2821 acatcgctta tgccctgcat gattcagagc atccggatca tgtcaccagt gacaatcata
     2881 cgcggaacgt gtggcgcacg ccggcgaaca ataagcttcg gatggaggat aagcgccagg
     2941 aagagcatat caagctcgca acggaatatg gcaaaacaca gctgaacatg ggacacctgg
     3001 tcaacagccg gcgtgaaaag cgaggtgccg gttttgaact gcgaacggat gagcatgggg
     3061 ctgtacgtgc agcaaaaggg ctgttcctga cggcagatgc ccaggccaga gcccaggggg
     3121 cggtgctgga aatggcggcc gcgataagtc agattaacca ggctaacagc cagatgcagg
     3181 ccctgaacag ctctgccgaa gccgccggcg ccctgctcag tgatatcaat acccagatta
     3241 ggtttgtaac cgacaaaatc agggaacttc agtcagcggt tctgctgggg agtgcgccgc
     3301 agggggtggc cctgacctca ggtgaacacc ttcagctctg cagtacccgt aataccatga
     3361 tcaatgcagg tcagcatctt gatatcggtg cgatgaaaaa tctgtcggta acggtggaaa
     3421 aagcccttgg tctgtttgtg cataaagacg gggcgaagct ggtagccaac cagggagata
     3481 ttgagattca ggctcagcac aacacgatgg cgctgttctc tgaaaaacag ctgacggtga
     3541 ccagtagtga agacgaaatt attatcagca ccccggaaac tctgacgctg aacggtggtg
     3601 ggtcttacct gagactgagt aagaacggga tagagcacgg ctcatcgggt gagtttattg
     3661 tgaagacgtc tgactatctg gtgcctggtt ctggcgtaga tctccccaac gaagcacctg
     3721 gtttcagcct gacggatatc acacaggaaa gcaaaatcag cagcaagtct tttaacgact
     3781 gacagggagt atgaatcgtg tctgtattac ccaaaatcgt ctggccgatc ccctccagcc
     3841 gtcggggaag tgagttcaaa aatcaggaag agatcctctc tcatttgggg ggagaatcta
     3901 ccgggctgta catgataggc cgtagtggca tgtggcatgg tgggatccat atcaccgaag
     3961 ccacgacacc atggtgtgcg cttagcggta agtctccact tgaagcgata gattttccgg
     4021 ttccttttaa aggtgagcag gcaatccgat gcatggctga cggagaagtg attgcttacc
     4081 gcatctgcag ggattatctg acgattgcat gggaatccgg tccgctgaat ttttccggct
     4141 catttgttct ggtgaaacac tatatccagc cgggtgagaa agaaaccagt ggactgcatt
     4201 tctatactct gtatatgcat cttgcgccgt attcagctta tgaggctgaa gcagaaaatc
     4261 agtgggtcgt tcaggatact ctaagagcat attctgaaat ggactggctg acggcgaaac
     4321 tgacttcaga tgaaacttct ccacaaattg cagggtacat gcctgaaggg gccagagtgg
     4381 agtgggaccc ggcagacagc aatcttaacg ctacaggaag taacaagaga aaatacggtc
     4441 ttgtgacgct aaagggttta ccagaaaaaa ccagcagtac tctcactccc ggtcagcgct
     4501 actgggttgt tgtggataac aacaatgtta agccagcggc gggggccggg cccggctggt
     4561 ggcgacactt gctgcctccc gcaaaggaag tcatggtatt cgataaaaca gtaagcctca
     4621 gttctccgtt tgccattaag gccggtgacc cgattgggca catgggctac tatcaggcac
     4681 caaaggatgg ggggtatgag gcacgatatc aggtacatat cgaatgcacg agtatggatg
     4741 acaatctgga gaaattcctg accaatcctg agcaggtggg aaaaaataat ccactctggc
     4801 tgaagtacgc cccggggctt gcgttgtaca agaaagatgt ttttacagaa acattcacga
     4861 aggacacgag agtgacggcc aggacgggaa ttctgccact gaaacaggtg caaacagaag
     4921 tcgataagtc gaccaaacag gaatactggc aattgcggcc agaaaacgcg tacgtgccaa
     4981 aaggtcaggt ggaacctcaa cttttgtcac agtatgatct ggcaaagctg gggttcagaa
     5041 ctgaaatcgc agagcctacc agctttgatt acctggatgg aaaaaaacag accactggat
     5101 tcttccgcag tcttatcaac tcactctatg aggctgcgaa cgatgatacg cgaaccagtc
     5161 atgcgctggt gaaacataat tatcagcgcc tgctggataa aattgatagt ggaagcgacc
     5221 gttactctcc aatggaatac tggagagcac ttcataaccc cgactatcgt gatgttcttc
     5281 agaaaacaat cgttaaacat cccagcgact ggtacttcaa aaaagacgat gcaatctggc
     5341 aaccatttct gaatgcgctg aaaaaagatg ctccggaatg gaagaaatac agcgaagatt
     5401 ttcttgataa gatggcctgg atgcaggatg tgaccacgga gaaactgggc ccttcgttgt
     5461 ggcatatgca tccaataatg tttttagcct ccctaattca acctaatgaa gatattagaa
     5521 ttcttcgttt acgtgcattt ctacgaatga tccgcatcgg tgaagggaca attcaggaag
     5581 acggatacag aacgatgttt actggtgtaa aatttacaga ttttagtaaa caccccaata
     5641 tcaggcatga agctaatggt gttgtttcga ctgctgctgg agcttatcaa tttttatacg
     5701 gaacatggag aaatttgcaa cgtagatatt catttccaga tttcagtccg agcaatcaag
     5761 atctcggttg tatagcgctc attgcaggtc gaaaagcatt agatgtagta atgcagggta
     5821 agattagtga agccatacat ttatgtcgta ttgagtgggc cagtttaccc ggttcaccgc
     5881 atggtcaacc aactgcaaat aaaaaaatga ttatggaaaa atatgaactc tacttagctg
     5941 aagaaaaaga aggtaaaaca agtttacatg ccaccacaga aaaaatagta aaattcattg
     6001 aaggtaatta cccagagtac ctatgaaaaa attcattttc ctgttactaa ttccagtgtt
     6061 ttgtaatgca aacactccta ctgttttcaa gtcttcttgc agtggtattc aatacagtat
     6121 cgaatcattt tgtagtgtaa ataatcttaa tgatgaccag gataaactta ccataccaat
     6181 ttgtagcaaa caaatattta cccttggtaa ggaaaatcat gacctatcag agtctacagg
     6241 ttttgttgaa cgtattaaca ttaaaggcga aaaaataaaa atgatgaatt tcgttttcta
     6301 cggggccaca tgtaaaagtg atagaatcat tttatccggc gcaggtggtt gtaattcatg
     6361 tggtgaaatg ttcaagatat ttaatttatc aggaaaagaa attaatgaga aatctacaat
     6421 gctagatttg gaagctatgc aggatattat tcccgatt
//
LOCUS       DAZGCQ010000199         6453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000199 DAZGCQ010000000
VERSION     DAZGCQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.199"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1375
                     /locus_tag="ACHK07_004937"
     CDS             <1..1375
                     /locus_tag="ACHK07_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8105149.1"
                     /translation="VRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERL
                     SAIEQEENELMTARDDLEQQYARECELTGELLESRSDISRQSETHHLQQALHDIQQNQ
                     PLLSVDVDVRTVAGVVADRTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQ
                     RLRAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEP
                     HTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGF
                     MRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPAL
                     LARFRTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPD
                     TGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWNDEDGIVIAFDGINSDYPQ
                     ESIYGQC"
     gene            1362..2003
                     /locus_tag="ACHK07_004938"
     CDS             1362..2003
                     /locus_tag="ACHK07_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105150.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            2000..2227
                     /locus_tag="ACHK07_004939"
     CDS             2000..2227
                     /locus_tag="ACHK07_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105151.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            2306..4825
                     /locus_tag="ACHK07_004940"
     CDS             2306..4825
                     /locus_tag="ACHK07_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8105152.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLELALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            4822..>6453
                     /locus_tag="ACHK07_004941"
     CDS             4822..>6453
                     /locus_tag="ACHK07_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO8105153.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQR"
BASE COUNT         1683 a         1354 c         1773 g         1643 t
ORIGIN      
        1 tgtgcgtatg agcctcgaca cggtgccgga acagctgacc cgtatccgtt cacagctcgc
       61 ttccctcggt atggaaaaac aggcactgct ggaagatatt gccgttggtc atcagaatca
      121 cggcgaacgt ctgtctgcca ttgagcagga agagaacgag ctgatgacag cacgtgatga
      181 cctggagcag cagtacgccc gggagtgcga actcaccggt gagttacttg aaagccgcag
      241 cgatatttcc cgccagagtg agacacatca cctgcagcag gcactgcacg acattcagca
      301 gaatcagccc ctgctcagtg tggatgttga cgtacgcacc gttgccggtg tggtcgcgga
      361 ccggaccggg gtgccgttat cttccctgat gaaggacgaa cagacggaac tgctgcatct
      421 ggaaaaggat atcggcagac gggtggtcgg acaggacgtg gcactggaat ccattgcgca
      481 gcgtctgcgt gcggcgaaaa cgggtctgac atccggtaac ggcccccagg gggtgttcct
      541 gctggtcggt cccagtggtg tgggaaaaac cgaaacggca ctggcgctgg cagacgtgat
      601 gtacggcggc gaaaaatcac tgattaccat caatctgtcg gaataccagg agccccatac
      661 ggtttcccag cttaaggggt caccgcccgg gtacgtggga tacggtcagg gcggtattct
      721 gacggaggct gtgcgtaaac gcccttacag cgtggtgctg ctggatgaag tggaaaaggc
      781 ccaccgggat gtgctgaatc tgttctacca ggtgtttgac cggggcttta tgcgcgacgg
      841 tgaggggcgt gaaattgact tccgtaatac ggtcattctg atgacctcca atctgggcag
      901 tgaccttctg atgcagcaac ttagcgaaaa gccggagaca acggaatcgg agctgcatga
      961 gcttatccgt ccactgctgc gcgaccactt ccagccagca ctgcttgccc gtttccggac
     1021 cgtgatttac cgtccgctga cgcagtctgc catgcgcacc attgtggaga tgaagcttgc
     1081 acaggtctgc gaacgcctgc actgccatta tgggctgagc acatcggttg atgaacgtgt
     1141 gtacgatgcc ctgacatccg cctgcctgct gccggatacg ggcgcccgga atgtggagag
     1201 tctgctgaac cagcaactgc tgccggtact gagccggcag ttgctcagct atcttgctgc
     1261 cagacagcat ccgacgtacc tgtctctgac gtggaatgat gaggatggta ttgtgatagc
     1321 gtttgatgga atcaatagcg attatccgca ggaaagcata tatggacagt gctaaagaaa
     1381 ttgcccggga tatgctggag gccgtaaata ttaatgggga tgggtttctg ggggccgtcg
     1441 ggaagggact catatctttg ccagtcagtc tcggttatat gggatatgat tttatagata
     1501 ccgggcatag gcgggaaaac cagaatgaca aattcagaat ggccagactg gtagaaaaag
     1561 gcattttcag ccgtgatatt attgggaaaa ttatcagtat ttgtcttgaa gactttgtat
     1621 cccggatcaa tatggaaaaa acaggctcca ttgtgaagaa cgtttcagga gatctggcag
     1681 gtagaatggc atttgctgaa ttgaccgggg ttaaactggg agaggttctg gtatccagag
     1741 ggctggctgc tttttttgca gggagttttg ccgggttgtt attatctgtt ggcgcagaga
     1801 catcgcgtgc gatttacaca tcccgttttc tgaaagacag aaatccggtg ttgcatgata
     1861 agctgaaagc tcagggggat ttggatcttt tgtactttct tgttgaagat attgtgcgcc
     1921 cgtttgaacg agcctgtatt gtcgcagacc gaaattcggc tgagtttgac cggatatgcg
     1981 aatatttttt cggtggttta tgaagataaa aagaacatta cttataatgc tttcccgatt
     2041 tatccgtggc gcaggtatgg ggttaggtgc gtcgggtatt gtatttacag tctggttctt
     2101 ttttctctca tctgctgaat ttaaatatct gtggggaggg ctctctgttg cgttattttt
     2161 tgtcggttat ctgatgtatc gatttgcata tgcatatatt tacgatgaat gggatgatta
     2221 tcagtaattc tctctgaaaa taaagcttaa cggtcatcat tatataattg cagttcaata
     2281 ccggtacaga catggagtta acaatatgag tctgacagat acactttcaa aaactatctc
     2341 tgcttccctg aatcgttacc atttaattat tccctcctgc ccgtcaccac tggatgttga
     2401 gcatttcagc ggcagggagg agatgagtca gacctattat tacatcatca attttaccag
     2461 tactgacaac gacctggatg ccgggctgct gctgcgtaaa tcagccacat ttacaatggg
     2521 tacgggaggg cttaaggagc aggtgagcca gaaagtagta catggtgtca ttacagattt
     2581 tcgtcgtata tcgggctccg ccgaccaggc aaaataccag gtgatactgg aaccatttat
     2641 taaactactg gacaggcagc atcgttctca ccgatttttc gttaacaaat cggtgccgga
     2701 ggtggtgaca gaggttctcc aggagcacgg cctgaaaggc tgggagtttg agttccgtct
     2761 caaaaaaacg tatccgaagc gtgaacaaat taaccagtat caggaaagtg acctgcaatt
     2821 tattcagcgt ttgctggctg aagtggggat tttttatttt ttcaccctgc aaccggacac
     2881 gcagacggaa gtagtgcatt ttggtgacag ccaggctgcg ctgacgtttg ataagacgct
     2941 ggcgataaac agtccgtccg gaatgaatga taacagagcg gattcagtgt gggggctgaa
     3001 tgtgacgcac aatgtggcag aggccagtgt caccacaaag gcttataacc atcgtgaagc
     3061 acaatatctg ttgcagtccg ccccggcaga catgacgcat ggtgatggtg acggaattaa
     3121 ctatggtgag gtgtaccact accatttccg tcatctggaa cggggggata aaatcgaccc
     3181 ggtgccggag acggccaatt tttatgcccg ccttggtcat gaacgttatc tggcggagca
     3241 ggtgcggata accggcaaca gtacagacgc cacgctggca cctgctcagg ttctgactat
     3301 caccgacagt cttccgccga cgttgcctgc gctgctgcgc aatccggtgt tgcttacctg
     3361 tgtcggtttt tctgcaagtc gcaaggatgc cctgcaggtt gtactgaagg gcgttcctta
     3421 cagtgaagtt atctgctggc gtcctccttt actgccgcgt ccgaaagtta ccggtaccat
     3481 gacggcccgt gtgaccagtg caaaagaagg ggatatctac gcctggcagg atgcatcagg
     3541 tatgtaccgg gtgaaatttg atgctgaccg tgatgataaa aatccgggac aggaaagcat
     3601 gccggtacgt ctggccaaac cctacagtgg tgatgcttat ggttttcatt ttccgctgat
     3661 tcagggaacg gaagtggcca ttgcctttga ggaaggtgat ccggatcgcc cctatatcgc
     3721 gcatgccctg catgattcac gtcatgttga tcacgttacc gataagaacg gtacgcgcaa
     3781 tgttatccgt acgccggcaa ataacaagtt gcggatggag gacaaacgcg gtgaggagca
     3841 tatcaagatc agtaccgagt ttggcggtaa gacacaactt aatcttggtc ataacgttaa
     3901 tgcctcccgg aaactcaggg gagaaggcgc tgagctgagg actgatgact ggataagtat
     3961 ccggggagga aaaggaatat ttatcagtgc agatatgcag cctcaggcac aaggaaagat
     4021 gctggatatg aatgaagcta tccgccaact ggagctggca ttatctctgg cccgaagtat
     4081 ggcgaaggcc gcaactgctg caaatgctac tcagggagat ataagttgtc agcaacggtt
     4141 gaattcatcg ctaacagatc tcactgcgcc gggaatgctg ttgcatgctc ctgatgggat
     4201 tggcatggtt agtgcacggg cgttacgaat tgcttcgggt agtgaaagcg tgggcataat
     4261 gtcgggagat aacactgata ttagtgccgg gcagtcattt acagttgtag cagaaggtgc
     4321 tgttagcctg ttgagcagaa accaggggat gcaactgtta gcagcaaaag ggcgggtaaa
     4381 tattcaggct caaagtgacg atttgtcaat gagttcccaa cagaatcttg atattcaaag
     4441 ttctgagggc aaagtgactg tttctgctaa tcaggaactg atactggcat gtggtggagc
     4501 ttatatcaaa ctaagcggtg gaaatattga gctgggatgc ccgggacaga tcctgttaaa
     4561 aagcacgaat atgcagaaga tgggaccaac aagtcttgat attgcgtctg ttgaaatgcc
     4621 gcgagggttt ggtggcggtt ttattcttac cgatgaagcg ggagtgccgc aaccttcgac
     4681 gccttatcga ttaacaacgg ctgaaggtga tattctacag gggatcaccg atgaaaatgg
     4741 taaaacagcg ccagtcaaca cctccattcc gtcagtagta aaagttgagt ttgggaaggt
     4801 aaaaattcat ggagaaacag aatgacaaaa tatcagggat atgatgtaac agatgccacc
     4861 cataaaacca gtattcataa tgactggaaa gttgttgttg caaagaaaaa accggcgcgg
     4921 ggtgtgactt taaccattgg tatatttttc gatggtaccg gaaacaatcg ggagaataca
     4981 gcatctcgtc tgatgaaatt taatgagtgt agtgcagcca gacagggggt aaatcaaaaa
     5041 gatgcacaaa gctgtgagga ctttcttgaa gaaattaaca agaacagtat ctcgaatggc
     5101 agttatcgtg gatattattc caatatccac tggctaaatt cactttacgt agcagatcag
     5161 actttgttgg aaagtcaggt tgaagcacaa attcagactt acatttccgg gatagggact
     5221 gcggctggtg aagcagattc agtcattggc atgggattag gcaccagtat tctggatata
     5281 tttgaaggtg tggtgacgaa aaccgacgag gcaatggaac gaatcaccca agcgttatca
     5341 gaatttatgg ggttcaattt aaatcctgat ttttgtatcg caaaaatcca gtttgatgtt
     5401 tttggtttta gccggggagc agcggcggca aggcactttg ctaatcgggt tatggaacag
     5461 gatcctgcta tagccagagc tattgctaaa gggctcagag gcgattttta tgatggtaaa
     5521 ccttcgggtg aagtccgttt tctggggctt tttgatactg tcgcagctat tggtggaatc
     5581 agtaatttct tcgatattaa tggacgtagc aacccgggag taaagctgga attgcgtcct
     5641 tccatcgcga agaaagtttt ccagattact gcaatgaatg aataccgata taatttcagc
     5701 ctgaacagta ttaaaggtat gtggcccgaa ctggctttac caggggcgca ttcagatatt
     5761 ggaggtggtt acaatccggt aggttcacct ctgcaggaaa atgaaagtct gtttctgagt
     5821 tgcccggcat ttgaaattgt cagtgacgat accagagaaa cagatacgcg agtataccgt
     5881 aatgcagagc aagccagaaa aatgttgatg actctgccag ccttaaaaca tattttgcct
     5941 catggaaaac taaccaccaa aattcgttcc gtaggtgtga acaacagcaa tcaacgtcgg
     6001 gctggcgtga tacaaaagca ggtgggagcc gcagtatttt ttgagcgtat ggcagtacct
     6061 aatgactggg ctaatgtttg cctgcgtgtg atgctggatg ctgctcagga agcaggggta
     6121 ttgtttgctc caattgaccc caaaaatcca gatatgaatt tatctcctga attaattcct
     6181 tttgttgata aagccattgc tcagggaaaa gcggtacgtt tggggcaaga gcctcaggct
     6241 tttactgagg aagagttgta tattatcgga aaatacaccc attgttctgc caactggaat
     6301 attgagtctg atggtaattt atgggtggat cctacaacgg gagagatttt tatccatcgc
     6361 tttggtccta aaggtaataa ggcgttcgtt tttccgaata aaccaaatga tcgctggatt
     6421 cgttctgtat ggtacatgga tgatcagcag cga
//
LOCUS       DAZGCQ010000200         6291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000200 DAZGCQ010000000
VERSION     DAZGCQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.200"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2151)
                     /gene="ypjA"
                     /locus_tag="ACHK07_004942"
     CDS             complement(<1..2151)
                     /gene="ypjA"
                     /locus_tag="ACHK07_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO8105154.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGELTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGTLDVREKGSATGILQSTQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLAGTEAHDST
                     VGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLITPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTTNELSGSGNFY
                     LHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQ"
     gene            complement(2216..2335)
                     /locus_tag="ACHK07_004943"
     CDS             complement(2216..2335)
                     /locus_tag="ACHK07_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8105155.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2296..2481)
                     /gene="ymgJ"
                     /locus_tag="ACHK07_004944"
     CDS             complement(2296..2481)
                     /gene="ymgJ"
                     /locus_tag="ACHK07_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO8105156.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            2582..2755
                     /gene="ymgI"
                     /locus_tag="ACHK07_004945"
     CDS             2582..2755
                     /gene="ymgI"
                     /locus_tag="ACHK07_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO8105157.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            2757..3101
                     /locus_tag="ACHK07_004946"
     CDS             2757..3101
                     /locus_tag="ACHK07_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO8105158.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3105..3440
                     /locus_tag="ACHK07_004947"
     CDS             3105..3440
                     /locus_tag="ACHK07_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO8105159.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3496..6144)
                     /locus_tag="ACHK07_004948"
     CDS             complement(3496..6144)
                     /locus_tag="ACHK07_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HFO8105160.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYADQFIHHDEYKHAVEIKDGEVK
                     DIDNALVITTGNGSYGVSVAGEGSNLAINNGVIVTTGGLYPSDNSSLSTALTASAIVS
                     EFGGAVILQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFSDGDNTFI
                     VTAGNHAVGVLACASPGSARACVSSLDEESTADTGNNENSAKAILDMAKGEITTHGTE
                     SYAAYANGTVVKSGDKLDYTNASVTLTNVDITTHGDNAHAIAARQGTVSFNQGEIYTT
                     GPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDVLSGSSLRSANEIL
                     YHKNETSNVTITDSEVSSAADVFINNIKGHLTVDTTNSKITGSANLSTDDNTHTCLSL
                     SDNSTWDIKADSTVSKLTVDNSTVYISRADGKDFEPTRLTITENYVGNNGVLHLRTEL
                     GDDNSATDKVVINGNTSGTTRVNVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIF
                     AGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGETRYIDPVTEQVRSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYA
                     TWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQQQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEK
                     NSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1589 a         1567 c         1390 g         1745 t
ORIGIN      
        1 ctgtgggctg acgccggtat cctgaacaaa gattttaaaa ttaccagtgg cgttgttgtt
       61 gacgaccagt tgatcgcccc gggagccagc aacgttggta tgcaggtaga aattaccgct
      121 accggaaagt tcattggttg tcagcgtatt gtagataccg ttagttgtgg caggcgctgc
      181 tgtttgtgct gataaataaa cattaccgtc gttaagtagc aaactgttta ctctatactc
      241 gcagttgctg gtgcctgcac aggaattatt gctgttaagc cagacactgc cccggctggc
      301 agccgtcagg tcggcaagcg tggtatcgac gccatttccc agagtcaatg tcgcgctatc
      361 ggtaatccgg accgcccctt caagttttac tggcgtaaca ttatcccgtg gcgtgattaa
      421 cgacaggctt cctgtcgcgc cactgaccga tgcatccgcc agcgtacctg cgtagacgat
      481 tgccgtcccg ccagcgacct ggagatcttc tgcccgggcc agaggctgaa actcatcttt
      541 tgaccgccca agggtatatt gcccgccaga gttaaccttt gtggcggagt cctgacccag
      601 gttttgcatt gcaccaccct tgccaaccgt tgagtcatgg gcttcggtac ctgctaatac
      661 caataaatta ccaccatttt ccagcaacat attggtcgct aaatttccgg caatggaaaa
      721 ggtgccgtac tggtgagtac cgctgatttc aataccgtta gccgtgctcg tctggagagc
      781 ggcaccgctg ttctggacga tatctgtcgc tttgccatta tcgttgactg tcagcgtacc
      841 gccttcattg atcgttgttt ttattgcctc accgttagcc gaaactgttt gtattccgcc
      901 gtcgttaatc gttgtctcat tcgccacacc ctcgacgatt tgttcgccgc cggtgagcgt
      961 cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt ccgcccgtat tgacctgtgt
     1021 tttggcggaa gtggtgtctg actccacggt taacacgccg ccatttgcca gcaggatatt
     1081 gttcgccgca ccctgctcga tgctgaacgc gacgccatca gcgcgtgtcc ctgtgacacg
     1141 cgtcgccctg gtggttgcaa ccaacgcgcc ctgagtactc tgcagtatcc ccgttgcgct
     1201 gcctttctcc cgcacatcga gtgtgccgcc attgttaagt ctggtggtat atgctacggc
     1261 ctcctcgtgg acaatcagct taccgccagc atcaacgatg gtatctaccg ccgaggtttg
     1321 agctacgacc gtcagttcgc cggtattttc cagcacaaca tgctcagccc tgcctccggt
     1381 aatagtgaag tgagatgacc tctggtatcc gtcaatatca gtccccgcgc ccgtgttggc
     1441 aactaaagca ctccccgtgt ccaggtaaac cccatgtgcg ataccgccgg tatcgacaat
     1501 cagcgtgcct ccggcgcgaa tattgctgcc agttgccgta cctcctttct caacaacctg
     1561 cttgctcccg gaagagatat ttgttgtgtc agctttcccg ccgctcttga tattttgcgt
     1621 gccactgttg atgttggttc ccgtagcaat accatgatta taaatattct gtgtgccgcc
     1681 attaattatg gtattagtcg cgttcccggc aacatccatg gccccgccgt tatctattcg
     1741 ggtcgcatca gctttagcat tcgttaaaac tgacattgtt cctttatcgt taattatcgt
     1801 ccctgtagaa attcctccct cactaattgt ctggctgcca ccgttaatga ctgtattatt
     1861 agcctgcccc tgataagagc catgaccaga tacctcttga tgtccaccag tatcaataac
     1921 acttccatta gaaatcccgc catatacctc ctgattgccg tgattgatga tatgagcatt
     1981 atttgttgta cctcgttcat ctactctttg gctgccgtct acagtctcat catttacaac
     2041 gccaataaca ttagaagtga aggccgtcat cccaggcggg gcatatatca tggcagctat
     2101 caataaggaa agtactgagc ggtgaaaata atagcgactg attctgttca taaatttcat
     2161 cctctgaaaa atgaatactg agtagcgtct aagtaaccta agctcggctg caacatcagc
     2221 ccgcaggcac cagaccaggg gattcatcct gaagagacag cgcaggtgta ttgtgttcgc
     2281 cgctcaccaa agacatcatg atgaaatgat gatatcccgc ataagaaaga ggcatttttt
     2341 aaacgcagtg cgctgaagtg tagttggata aaaaatcact cttttcagat aatactgtcg
     2401 tacgcttatc tttcgacaat gaggccgtcg gcaaaataaa atgactaatg taatcgttgc
     2461 tgatgaatat cttctgctca cataaaaatc acacaataat attgataaat cagattatct
     2521 tacctttaca acattgcgtt aacagtcaat gacacttctt gaataaaaat attgtcaatc
     2581 aatgagttat tcctcattta agattattct cattcacgta aaaaacataa ttcctatcat
     2641 cacagccacg ctcattttga attatctgaa taatagcgag cgttctttag ttaagcagat
     2701 cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca tctaagttga
     2761 agaaaaaaat acttgccttc gggcttatct cagcattgtt ctgctccacg ccagcgatgg
     2821 cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc aggcgttggt ttacttactg
     2881 gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt ggtgcagtaa
     2941 cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc aaaagtgggt gccgccgttg
     3001 gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa ggtgccgtga
     3061 ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg atgaaaaaat
     3121 ttgctttact ggcgggtctg tttgttttcg caccaatgac ttgggcgcag gactacaata
     3181 ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc agaagccaac gaaatggcaa
     3241 aaactgacag cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc agcgttgcca
     3301 gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggtc gaaaaactga
     3361 atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat gacaccatgc
     3421 gtgctatcgg caaaaaataa tatttaatat gttaccgcgt cgatgaattc gacgcggtat
     3481 tttagctaac ggggatcaga acttatattt caggcccacc atcactgcgg tgtcattgta
     3541 gccattatca cccagctgca cgcccacatt accccagacg ctggccgccg gattcagatt
     3601 gccatttacg ccaagtttaa tttcccccag attacgcgcc ccgtcttgtt cgactttcac
     3661 accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt caacaaaagg
     3721 ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa aggtgcggaa
     3781 accaagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc cattggcttc
     3841 cgtatgcgca ttatgatcga cgcccatcca gatagcctgt gcctgttgct ggaagatcca
     3901 cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaagccgt aacccgcttc
     3961 cagcgagacg gttgcgcctt tcgcgctata ggattcatag gctaattcat cccctttcac
     4021 cgagttttta aaccagctat attgcgccca ggcgtcaatg tatgcgcctt ttttactaat
     4081 gtcatcggca aaccaggtgg catacagccc tgcgctatag cctctgacac tccctttcga
     4141 acgataatcc gacacgctgg aatgagttga gttgtagtcg cgggcataac cagccatcac
     4201 tcccaaacgc caactgtcac tgtcggtaaa accaccggtt aacagatcgg cccctaactg
     4261 cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg
     4321 cccgccaatt tgacgtagcc aaagtcggct tgaacgcacc tgttcagtta caggatcgat
     4381 gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aacagagtgt tagcggctgc
     4441 caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtgggcg caacagtagg
     4501 cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt tagtcagata
     4561 ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt aggcaccggc
     4621 gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc tgataatctc
     4681 tatcccattt aacgtataag ccccgctgcc gccagcattg gtaacgttaa ctcgggttgt
     4741 tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat cacccaattc
     4801 agttctgaga tgcaatacgc cattattacc aacgtaattt tcagttattg ttaatcttgt
     4861 tggttcgaaa tcctttccat ctgcccggga aatatagacc gtactattgt caaccgtgag
     4921 cttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa gcgacagaca
     4981 ggtatgagtg ttatcatctg ttgaaagatt agctgaaccc gttatttttg aattagttgt
     5041 atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg atgatacctc
     5101 actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt catttgctga
     5161 ccgtagtgag ctaccagata aaacatctac agttgcttcc tgaccattta tggaagactc
     5221 cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc
     5281 gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt ctccctggtt
     5341 aaatgaaact gtcccctggc gagcagcgat ggcatgagca ttgtccccat gcgtggtgat
     5401 atcaacattg gttaatgtaa cgctggcatt ggtataatca agcttgtcac cagacttgac
     5461 gacagtgcca ttagcgtaag cggcataact ttctgttccg tgggttgtta tctccccttt
     5521 tgccatatcc agtattgctt tggcgctatt ttcattattt cctgtatcgg cagtactttc
     5581 ttcatcaaga gaagatacac acgcacgagc gcttccggga gatgcacacg caagaacgcc
     5641 gactgcgtga ttacccgcag taacaataaa agtattatct ccgtcagaaa aagagggggt
     5701 aaccgaaccg tcagagttga ctcttattat ggtatcagta ttcaggtttc cactcgtttg
     5761 acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac cctgcaaaat
     5821 aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgtgc ttagagaact
     5881 attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa tggctaaatt
     5941 gctaccctct cctgcaacag atacaccgta acttccatta cccgttgtta taactagcgc
     6001 attatcaata tcttttactt ctccatcttt tatttccacg gcatgtttat actcatcgtg
     6061 atgaataaac tgatcagcat atgcattacc caccgccaga gcaataagtc ctaaactaaa
     6121 cccggggagt tttttcagtt tcatagatta accatccatt cattggatta acaaacattc
     6181 ctggtcaaat aacctatgag ttcagattac caaaacggag ggtaaagaca aaaacaaaag
     6241 cacaagaaat ttaaatagat aaacagcgac aatatataaa tgataaataa g
//
LOCUS       DAZGCQ010000201         6278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000201 DAZGCQ010000000
VERSION     DAZGCQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.201"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..925
                     /locus_tag="ACHK07_004949"
     CDS             <1..925
                     /locus_tag="ACHK07_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8105161.1"
                     /translation="EQVSDKLREKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSL
                     TDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLA
                     DKTHQILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHMLEQGYKNIGFIGAH
                     MDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVIC
                     SHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGK
                     LLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1128..1808
                     /locus_tag="ACHK07_004950"
                     /pseudo
     CDS             1128..1808
                     /locus_tag="ACHK07_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1825..2133
                     /locus_tag="ACHK07_004951"
                     /pseudo
     CDS             1825..2133
                     /locus_tag="ACHK07_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2236..3342)
                     /locus_tag="ACHK07_004952"
                     /pseudo
     CDS             complement(2236..3342)
                     /locus_tag="ACHK07_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4109..6157
                     /gene="ireA"
                     /locus_tag="ACHK07_004953"
     CDS             4109..6157
                     /gene="ireA"
                     /locus_tag="ACHK07_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO8105162.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1787 a         1382 c         1452 g         1657 t
ORIGIN      
        1 ggaacaggtt tctgacaagc tgagggaaaa gatagaacag gccgtagaag agttgggtta
       61 tattcccaac agaactgctg gcgcccttgc atctggtcac agtcatacag tcgccgtact
      121 ggttccttcc ctgacagata aaaccagctc ccgctttatg cagtcgttgc agcaggtgct
      181 taataaaaat gaattccagc tactgcttgg gtgtcatgag tataatcagc tcaaagaagc
      241 tgaaatcctg atgacgcttc ttcaaggtaa cccggcggcg ttagttattt ttggttctca
      301 gttggctgat aaaactcacc agatactcga gaaaacaaat atacctacca ttaacgttgt
      361 cggttcccct ttcagtccgg ctcaaattac catcgaaaca gcattttttg aagcatcaca
      421 taaactgacc gaacatatgc tggaacaggg ttataagaat atcggattta tcggtgcgca
      481 tatggacaac cgcctgcaac gtcagcaact gaacggctgg cataaagcaa tgctgagcta
      541 ttaccagaac tctgatcttg ttatcaccat gccgggtgcc gcaagtttgc aattggggcg
      601 ttacgcatta aacgagatat tactgcgaca accagagctg gatgctgtga tttgcagcca
      661 cgaagagatt gcgctgggta tcatgtttga gtgccagcgg cgtttgttaa aaatccctgg
      721 aaatatcgct gtagcttgtc ttgacggttc agacagttgc gatcaaacac acccaacctt
      781 gacatccatc cgtattgact acaaaaaaat ggggacagag acgggtaagc tgctgattgg
      841 actattgaat aataatcatg atgaatcaga agagtcgcgt atcgtgcaat tcaactacca
      901 gatagaatta cgtcagagta catgagctca aaaggggatt attgcaaagt tagctattgt
      961 ggtagggata gccgtttccg gctattcccc gcgcagagcc ggatgtgtgc tgctaacgca
     1021 tcctgtcaac gacggatgaa aagttatcca cttatatctc caccaacggc ccaatattgt
     1081 accgttcgtg acacatccat ttctacttca ggtatttggc gtagtagcgg gtgctaaaaa
     1141 tttgccgcaa aaccgcaata tcagcccgca gaggcgctct ccagattatc cattcctata
     1201 ttatctctgc accggatgaa ttactcgaac aactcagaaa tatgacacat atgcaactta
     1261 tcaggactct gggatcctgg aggcctgatg ccagtgaata cagtaatgtt accaacgttt
     1321 atcgcattac attgaaatcc cttgttcgac gctatttcga gtttcatgat gaaatcacca
     1381 acttggatgt catgattgcg gcaattgttg atgaactggc acctgaactg attaaacgta
     1441 atgctgttgg atacgaaagc gctttgcagt tgttgatctc tgccggagac aatccctaac
     1501 ggttaagaac cgaatcaggt tttgcggcgt tgtgcggtgt cagccagttt cccctatctt
     1561 ctggaaaaac gaaccattac cgacttaatc ggggtgggga gcgtgttgca aatagtgcac
     1621 tttacatcat cactatagga tgtttacaaa ctgacgataa aacaaaggaa tatgtctcca
     1681 ggcgagtagc cgaaggacat acaaaaatgg aagcaatacg ctgtctgaag cgctatatct
     1741 cacgcgaagt ttatacatta ctacgaaatc aaaacaggca tgttaactgc atcccgataa
     1801 cgtcttgact cttagaaggg cgtccaaggc agtcattatt cgagcaggca gttccggcag
     1861 ttattgtgtc gataccagat caggcagagt atgagccggc gcggaaaatg ctgggataac
     1921 agctcaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt ggtgggttac
     1981 gtaagcttca gcgaggcagc tcactccata acggacgata tcgttggata ttacagcgca
     2041 ctaagaccgc acgaatataa cagtgggtta tccccaaacg aatcggaaaa tcgatactgg
     2101 aaaaactcta actcggtggc cagtttttgt tgaccacttc agccatcgcc agacgctaac
     2161 atgagttact cttgttctga gctacgtcac cttcagggaa ctggttatcc tcattcttta
     2221 tttgtgacat tgtggttatt gtgcgacaaa cgtggctatc ccgccccata tcccagataa
     2281 aaccacctgc tcacgtgcga cagcgacaat tgtaatattg acattctttc ctttggcctg
     2341 aagttttcga gacctcttgc aaagcctgag ttgagcatcc catgctctgt caatgacggg
     2401 gcggggtaaa ttttcctgat tttttgtatt gccggactga tgcgtgtcgg atgccggtaa
     2461 ctccacgctg cctcaaccaa tagctttctg gcatagctgt ttccggcttt ggtaatgctg
     2521 ccctgatgtc tgcttccacc cgatgaatat tctgatggcg tgataccaaa ccatgacatc
     2581 agttgtcgtg gatgttcaaa acgggtcaga tcacccagtt cagaaattaa tccaaccgca
     2641 gtaataaact gaatccctcg catggcttgc tgcgcctcta ctaccggata cagcctccat
     2701 tcggtaacgg cctccttcgg gcggattcca gccgttcaca ttgtgcctgc cggtcttcta
     2761 ttgtacaacg gtgttcgtca aaagctaatt gtcgccaggg actttcgaat gagtatttgc
     2821 tgagccagtg tcggtgtgca gggccccaat ctgctcgccc gacataatgg accccatgaa
     2881 ccagaagaaa cgatttcaga cgttgccttg catgccgtaa atcatcgcgg gcagatgccc
     2941 atgcccgggc caaatcccgg aatgcctcat cttcaacgcc ggatacgtag acagcagaaa
     3001 gatctcctgc acgcagtgaa cgcacaagtc tgatggcatc acggtgtcgg ttttaccccg
     3061 ctcccccggt tttttcggga taagtgaggg ggcgcagacc atgcagtcaa aaccggattt
     3121 caccagccga cgataaagtc cataaccaca gggtcccgct ttataaacga tactgacctg
     3181 cgatgacttt gacctgatac gttgataaag attatgaatg tcagtaggtg atgtgctaat
     3241 tttacccatc agttcaacag gttctgaatt gatagcatag gagacagtaa tcgattcttt
     3301 gtgaacatct aattcgacgt acaacatgtt attgttgttc atgctggcct ccgtggtgga
     3361 aattccaggg gggtagcaac agctcaccca tgcggctctg acaacgatgt taacccgcat
     3421 taaatatccc acgttggaca gcatctgtct cacggaaagt catactgcct tacgtgcata
     3481 tttcacctta cgccttcact gtctgtcatc ggaagtactg atttatattg gggctttgca
     3541 cttactgtca ttcacgggaa aagatggtgg tgaacagtac gatactgtct tggctgttgc
     3601 cgagggaaga ttcaggagtg gtaagggggt tagtggtgta agatgagtct gaagctctga
     3661 ctggtcagtg acgcgaaact caatctacca gggtaaactc ccccttgctt ttgcgcagaa
     3721 gcagtgggcc ttggttcgct tatgtcctgc atctacaagc gaatgactga ccggagactc
     3781 ccatatctgg tcagtcgccc atttttattt tccaccacat cggacaagca taccgtaata
     3841 aacagcacca gtctcctgtt cactcttgct tgatttactg gaatgctgtt tatggagata
     3901 tccggttgaa agattttacc tcttcgacag gactataaat tatacagtaa ggagtccctc
     3961 gtagaaaggg gctgattctc atcaagtcgg acaggttcaa agtgttacct gaatgagcta
     4021 aatgttattt accttccttt ctgttcattg taaatttttt cttgctaacg caaatcatta
     4081 tcattacgat tttagaaaag ggaaatttat gaagaacaaa tatatcattg ctccgggcat
     4141 tgccgtgata tgttctgcag tcatatcatc aggttatgcc agttctgata aaaaagaaga
     4201 tacgcttgtt gttactgcct ccggcttcac tcagcaactc agaaatgcac cggccagtgt
     4261 ttcagtcatt acttcagagc aactgcaaaa aaaaccggtt tcagacctgg tcgatgcggt
     4321 aaaagatgtt gaagggatta gtatcaccgg tgggaatgaa aaaccggata tcagtatacg
     4381 tgggttaagt ggcgattata cattgattct ggtcgatgga cgacgtcaga gtggtcggga
     4441 atccagacca aacggcagcg gcggttttga agccggattt atccctcctg tggaagcaat
     4501 tgagcgtatt gaagttatac gtggccctat gtcttccctg tatggttccg atgctatcgg
     4561 aggagttatt aatatcataa ccaaacctgt taataaccag acatgggatg gcgtacttgg
     4621 gcttggggga attattcagg aacatgggaa atttggtaac tcaaccacga atgacttcta
     4681 cctgtcaggc ccattggtta aggataaact tggtcttcag ttatatggag gaatgaacta
     4741 tcgcaaggaa gatagtatct ctcagggaac accagcaaaa gataataaga atataacggc
     4801 aacactccag tttactccga ctgaaagcca gaagtttgtt tttgaatacg gaaaaaataa
     4861 ccaggaacat acattaacac ctggtgattc tcttgatgcc tggactatgc ggggaaatct
     4921 taaacaacca aacagtaaaa gagaaacaca taactcacgt agtcactggg tagcagcatg
     4981 gaatgcccag ggcgaaatac tgcatcctga aattgctgtt tatcaggaga aagttattcg
     5041 tgaggttaaa tctggtaaaa aagataaata taatcattgg gatcttaatt acgagtcaag
     5101 aaaaccggaa ataaccaaca cgatcataga tgcaaaagtg acggcatttc tgccggaaaa
     5161 tgtgctgacc atcggaggtc aatttcagca tgcagaactc cgtgatgact cagccacagg
     5221 taaaaaaacg acagaaacac agtctgtttc agttaaacag aaagctgttt ttatagaaaa
     5281 tgaatatgct gcaacggatt ctctcgccct gactggaggg ctgcgcctcg ataaccatga
     5341 aatctatggc agttactgga atccaagatt gtatgctgtt tataacctga ccgataatct
     5401 cactctcaaa ggagggatcg caaaagcatt tcgggcccct tcgattcgtg aggtcagtcc
     5461 tggatttgga acactgacgc agggtggtgc ctctattatg tatggaaaca gggacctgaa
     5521 accggagacc agtgtaaccg aagagatcgg tattatttat aataatgaca gtggtttttc
     5581 agcaagcgcg actctgttta atacagattt taaaaataag ctgaccagtt acgatatagg
     5641 cacaaaagat ccagtcactg ggctaaacac ttttatttat gataatgtgg gtgaggcaaa
     5701 tatcagaggt gtggagcttg caactcagat tcctgtgtat gataaatggc gtgtatctgc
     5761 aaactataca ttcactgact cccgtcgaga aagcgatgac gaaagtctca atggcaagtc
     5821 gctgaaaggg gaacctctgg aaagaactcc cagacatgca gccaatgcaa aactggaatg
     5881 ggattacact caggatatta cattttattc atctctgaat tatacaggaa aacaaatctg
     5941 ggcagcacaa agaaatggtg ccaaggttcc ccgtgttcgt aatggattca cttctatgga
     6001 tattggtatg aattaccagt tcctgccagg cacgctgatt aattttgccg tacttaacgt
     6061 cacagacaga aagagcgaag atatcgatac cattgatggt aactggcagg tcgatgaagg
     6121 acgtcgttac tgggctaatg taagaatatc cttctgagag aatatagata acctgaggcc
     6181 gtgcgcctca ggtaccagta tagtaatcag taacgtcagc ttccaggttg taatgctttt
     6241 aattttgata aaaataaact taataaatta catttgtt
//
LOCUS       DAZGCQ010000202         6270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000202 DAZGCQ010000000
VERSION     DAZGCQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.202"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..1223
                     /gene="emrA"
                     /locus_tag="ACHK07_004954"
     CDS             51..1223
                     /gene="emrA"
                     /locus_tag="ACHK07_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO8105163.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            1240..2778
                     /gene="emrB"
                     /locus_tag="ACHK07_004955"
     CDS             1240..2778
                     /gene="emrB"
                     /locus_tag="ACHK07_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO8105164.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(2842..3357)
                     /gene="luxS"
                     /locus_tag="ACHK07_004956"
     CDS             complement(2842..3357)
                     /gene="luxS"
                     /locus_tag="ACHK07_004956"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO8105165.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3507..5063)
                     /gene="gshA"
                     /locus_tag="ACHK07_004957"
     CDS             complement(3507..5063)
                     /gene="gshA"
                     /locus_tag="ACHK07_004957"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO8105166.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(5136..5564)
                     /locus_tag="ACHK07_004958"
     CDS             complement(5136..5564)
                     /locus_tag="ACHK07_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO8105167.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVATLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(5561..6127)
                     /gene="yqaB"
                     /locus_tag="ACHK07_004959"
     CDS             complement(5561..6127)
                     /gene="yqaB"
                     /locus_tag="ACHK07_004959"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO8105168.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1489 a         1687 c         1587 g         1507 t
ORIGIN      
        1 ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat atgagcgcaa
       61 atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt aagcgtctgc
      121 tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt tattggtttt
      181 tagtactgcg tcatttcgaa gaaaccgatg acgcatacgt ggcagggaat caaattcaaa
      241 ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc gattttgtaa
      301 aagaaggcga cgtgctggtc actctcgacc cgacagatgc tcgccagacg tttgaaaaag
      361 ccaaaactgc actggcttcc agcgttcgcc aaacccacca gctgatgatt aacagcaaac
      421 agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa agcgactaca
      481 accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg caacacgccc
      541 gcgacgccgt taccagtgcc caggcgcaac tggacgtcgc gattcaacaa tacaatgcca
      601 atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa caggctgcca
      661 ccgaagtacg taacgcctgg ctggcgctgg agcgtactcg tattgtcagt ccgatgaccg
      721 gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat tagcccaacg acgccgctga
      781 tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag acgcagattg
      841 ccaatatgcg tatcggtcag ccggtcacta tcaccaccga tatttacggc gatgatgtga
      901 aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc tcactgcttc
      961 cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctgcct gttcgtatcg
     1021 aactggacca aaaacagctg gagcaatacc cgctgcgtat cggtttatcc acgctggtga
     1081 gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt tccacaccgg
     1141 tagcggtaag caccgcgcgt gaaatcagcc tggcacctgt caataaactg atcgacgata
     1201 tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca aaaaccgctg
     1261 gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tggcgacatt catgcaggtg
     1321 ctggactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct gggctcatcg
     1381 ctcagccagg gaacgtgggt aatcacttct ttcggggtgg cgaatgccat ctcgatcccg
     1441 cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctctg gtccaccatc
     1501 gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca gcctaaatat gctgatcttc
     1561 ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca aagtctattg
     1621 ctgaataact acccgccagc caaacgctca atcgcgctgg cgttgtggtc gatgacggtg
     1681 attgtcgcgc caatttgcgg cccgatcctc ggcggctata tcagtgataa ttatcactgg
     1741 ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat gacattgcag
     1801 acgctgcgcg gacgcgaaac ccgcaccgaa cggcggcgga ttgatgccgt ggggctggca
     1861 ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa agagctggac
     1921 tggttttcat cacaggaaat tatcatcctt accgtggtgg cggtggtggc tatctgcttc
     1981 ctgattgtat gggagctgac cgacgataac ccgatagtcg atctgtcgtt gtttaagtcg
     2041 cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt atatgctcta cttcggcgct
     2101 attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcaac gtgggcaggt
     2161 ctggcctctg cgccggtagg gattattccg gtgatcctgt cgccgattat cggccgcttc
     2221 gcgcataaac tggatatgcg ccgactggta accttcagct ttattatgta tgccgtctgc
     2281 ttctactggc gtgcctatac ctttgaaccg ggtatggatt ttggcgcatc ggcctggccg
     2341 cagtttatcc agggcttcgc tgtggcctgc ttctttatgc cgctgaccac cattacgctg
     2401 tctggtttgc caccggaacg actggcggcg gcatcgagtc tctccaactt tacgcgtacg
     2461 ctggcggggt ctatcggcac gtcgataacc acaaccatgt ggaccaaccg cgagtcgatg
     2521 caccatgcgc agttaaccga gtcggtaaac ccgttcaacc cgaatgccca ggcgatgtac
     2581 agtcaactgg aagggcttgg gatgacgcaa cagcaggcct ccggctggat tgcccagcag
     2641 atcactaacc aggggctgat tatttccgcc aatgagatct tctggatgtc agccgggata
     2701 ttcctcgtcc tgctggggct ggtgtggttt gctaaaccgc cctttggcgc aggtggcggc
     2761 ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt ttttcaatta
     2821 attgtgaaga tagtttactg actaaatgtg cagttcctgc aacttctctt tcggcagcgc
     2881 cagttcttcg ttgctgttga tgcgcacgtc acgttccaga atgctacgcg caatatcctg
     2941 cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca gctccggaat
     3001 ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccagg catcagcaac
     3061 acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc agcccattgg
     3121 cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa aaccagcaaa
     3181 caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg gcacgcagaa
     3241 gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt tcgccacccg
     3301 aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca acggcattta
     3361 gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg cgagtctgag
     3421 tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa attttggcca
     3481 ctcacgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac cgcaaacggt
     3541 tcggtatctg cggcttccat ttcctgctga cggcgttcag acgcctcgcg ctcggctaca
     3601 aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg gtaggcttct
     3661 gcaaatgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa gatacgggca
     3721 gagaaagtca gatcgggatt atcgaagcag gcgaccagtt catcacacac tttctgatac
     3781 gcttcgccgc cgttaatgct atccagcgtt tgcgcgacgc gtttcagatc gcggaacaga
     3841 tctttaccca cctgcggtaa cgggaactgc gcggtttcgc agccgatacc cagcgtcaga
     3901 cccggtttgc gaccttcgag gatcacccgg ttccagttaa cgcgtgtaca ggcaagttcg
     3961 ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag gtcgaggaat
     4021 cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag cgaacgcact
     4081 tcaatatatt caatgccgcc acgtaacagc gcatcagaag gcgactcgcc gctgcgggta
     4141 acgcgttttg gacgaatcgg cgcgtacagt tcgttttcaa tctgcagcac attgctattg
     4201 atttgcagcc gcttaccatc tttctcaata ccaatcttcg cgtactcttc cgatggcgtt
     4261 ttgattgcct gtttcaggcc cgctacgtac tcgtaaagat cgttgaaggt aataccaaga
     4321 ttgctttgcg atttattggt atagccaaga tcgctcaaac gaagagaggt cgcatacggc
     4381 aggtaataca taccgcactc ggttttctca aacggcagcg acgttggttt accttgcagg
     4441 aatgaagagc atattgccgg agacgcgcca aacagataag gaatgaccca accgaagcga
     4501 tagtagttgc gaataacgcg gaaatagccc gcagagattt tctctttagc atcagcgccc
     4561 gaaatatcgc cacacttcgc ttgccagaat gccataggca aagagaagtt gtaatgcacg
     4621 ccggagatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg gtacagcgtt
     4681 ttaaagcgtc cggtgttaga agtgccatac tgcgccagtt cgatgtcctg accttctgcg
     4741 atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatgttgcg cgccgtatag
     4801 cgatgcaggt cgcgcataaa ggtcagcata tgttcaatat caccatccac tggtgtaata
     4861 aattccagca atgcttccgc aaaatcggtg gttatccatt tatgcgtcag tgcggaacct
     4921 aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg caaagtttcg
     4981 cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag ccaggccagc
     5041 gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt ttaattagca
     5101 taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat catgccctga
     5161 acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa aaaaaagatt
     5221 accggccccc aggagatgcg catccatccc gctaacaggc acagtaaatc gccaactacg
     5281 ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc agtagctttc
     5341 tcttgccagc gcgatgtttt acgcaatgga aagaaacgcc caaggataac gttagttaac
     5401 cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggatg actgatcccg
     5461 gaaagcaacg ttgccaccag cacgacttcg gagttgccgg gtaatagtgt agcgctgaga
     5521 aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag cgaacatcca
     5581 cagcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct tcaaagacca
     5641 cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg tctggcgcgg
     5701 gtttatggtg tttgacgtga tcggcagcga cgacggcgtc aaaataacgg cgtaatccca
     5761 ggtgagccag caatgcctca gcgatggcgc tttcactccc cgttcctaca gccattgggc
     5821 gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca ctatccagca
     5881 gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg agatcggcct
     5941 gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta agcgcaatca
     6001 tcgcctgaac atcgtactga agaccgtagt gccctaatac ttcgcgccac gctttacggt
     6061 gcgtaggctc cgtatccagg attgtgccat ccatatcaaa aattaaacct gcataacgct
     6121 cgtacatggt cttctcgcaa gtcgaaaaat atgacgctac tttagcgtaa ttgcttgatt
     6181 ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt tatttagaaa
     6241 tgagatattt ttgaaggaaa ttttttggag
//
LOCUS       DAZGCQ010000203         6263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000203 DAZGCQ010000000
VERSION     DAZGCQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.203"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..1340
                     /gene="wcaK"
                     /locus_tag="ACHK07_004960"
     CDS             60..1340
                     /gene="wcaK"
                     /locus_tag="ACHK07_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO8105169.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FHDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAHQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSCERQTGMQMVQSVLERIGEVK"
     gene            1337..2557
                     /gene="wcaL"
                     /locus_tag="ACHK07_004961"
     CDS             1337..2557
                     /gene="wcaL"
                     /locus_tag="ACHK07_004961"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO8105170.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVDIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVVTPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2568..3962
                     /gene="wcaM"
                     /locus_tag="ACHK07_004962"
     CDS             2568..3962
                     /gene="wcaM"
                     /locus_tag="ACHK07_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO8105171.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4120..5115
                     /locus_tag="ACHK07_004963"
     CDS             4120..5115
                     /locus_tag="ACHK07_004963"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO8105172.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKSPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            5358..6245
                     /gene="galF"
                     /locus_tag="ACHK07_004964"
     CDS             5358..6245
                     /gene="galF"
                     /locus_tag="ACHK07_004964"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO8105173.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGIKFREKILKLL"
BASE COUNT         1670 a         1555 c         1569 g         1469 t
ORIGIN      
        1 aaaacggcaa cgaacacaac aaacacaacc tcacctggca tcaaatttat gaggaaaaaa
       61 tgaaattact tattctgggc aaccacactt gcggcaatcg tggcgacagc gccatcctgc
      121 gcggcttact tgatgccatc aacatcctca atccacacgc cgaagtggac gtcatgagcc
      181 gctatccggt cagttcttcc tggctgctca accgcccggt aatgggcgac ccgctgttcc
      241 tgcaaatgaa acaacacaac agcgcggcgg gcgttgtcgg gcgcgttaaa aaagtcctcc
      301 gtcgccgcta ccagcatcag gtattgctct cacgcgtcac cgacactggc aagctgcgta
      361 atatcgccat cgcccaggga ttcaccgact tcgtgcgcct gctgtcaggt tacgacgcca
      421 ttatccaggt cggcggatcg ttttttgtcg atctctacgg cgtgccgcag tttgaacatg
      481 cgctttgcac atttatggcg aaaaagccgc tgtttatgat tggtcacagc gtcggcccgt
      541 tccatgatga gcaatttaac caactggcga actacgtttt cggtcactgt gacgcgctga
      601 tcctgcgcga atcggtcagc ctcgatctaa tgaaacgcag caatatcact accgcaaaag
      661 tggaacatgg cgtcgatact gcgtggctgg tcgatcacca caccgaagat ttcaccgcca
      721 gctatgccgt ccaacactgg ctggacgttg ccgcacacca gaaaacggtg gcaattaccc
      781 tgcgcgaact ggcaccgttc gacaaacgcc tcggcaccac tcagcaagcg tatgaaaaag
      841 cctttgccgg ggtggtcaat cgcattctcg acgaaggcta tcaggtcatt gcgctctcca
      901 cctgtacggg tatcgacagc tataacaaag acgaccgcat ggtggcgctc aacctgcgcc
      961 agcacatcag cgatcctgcc cgttaccacg tagtgatgga tgaactcaac gatctggaaa
     1021 tgggcaaaat tctcggtgcc tgtgaactca ccgtcggtac gcgtctgcac tccgctatta
     1081 tctcaatgaa ctttgctact ccggcgattg ccatcaacta tgaacataaa tccgccggga
     1141 tcatgcaaca actggggctg ccagagatgg cgattgatat ccgtcattta ctggacggca
     1201 gcttgcaagc gatggttgcg gataccttag gccaacttcc ggcgctgaac gcgcgactta
     1261 acgaagccgt cagttgcgaa cgtcagacgg gaatgcagat ggtgcagtct gtactggaac
     1321 gcatcgggga ggtgaaatga aggtcggctt cttcttattg aaatttccgc tgtcgtcgga
     1381 aaccttcgtc ctcaatcaaa ttaccgcgtt tattgatatg ggatttgagg tggatattgt
     1441 tgcgctgcaa aaaggcgaca cacaaaacac ccacgcggca tggacgaaat ataaccttgc
     1501 cgccagaacc cgctggttac aggacgaacc tacgggcaaa gtggcgaaac tgcgccaccg
     1561 cgccagccag acgttacgcg gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa
     1621 acgctatggt gccgagtcgc ggaacctgat tttgtctgcc atttgcggcc aggtcgtaac
     1681 accgcttcat gccgatgtct ttatcgctca ctttggtcca gcaggggtaa ccgcagcaaa
     1741 actccgcgaa ctgggtgtca ttcgcggcaa aattgctact atcttccacg gcattgatat
     1801 ctcaagtcgg gaagtgctca accactacac ccccgaatat caacaactgt ttcgccgtgg
     1861 cgacctgatg ttaccgataa gtaatctgtg ggccggaagg ctgcaaaaaa tgggctgccc
     1921 gagggaaaaa atcgccgtat cgcgtatggg tgtggacatg acgcgcttta gcccgcgtcc
     1981 ggtgaaagcg cccgcaacac cgctggaaat catttccgtc gcacgcttaa ccgagaaaaa
     2041 aggcctgcat gtggcgatcg aagcctgccg tcagttgaaa gagcagggcg tggcatttcg
     2101 ctatcgcatc cttggcatcg gcccgtggga acgacgcctg cgcaccctca tcgaacaata
     2161 tcaactggaa gatgtggtag agatgccggg ctttaaaccg agccacgaag tgaaagcgat
     2221 gctcgacgac gcggatgtct tcctgttgcc atcggtaacg ggcgcggatg gcgatatgga
     2281 aggcattccg gtagcgctga tggaggcgat ggcggtcggc attccggtgg tttctactct
     2341 gcatagcgga ataccggaac tggtggaggc tgacaaatcc ggctggctgg tgcctgagaa
     2401 cgatgctcgc gcactggcgc aacgactggc ggcgtttagc caactggaca ccgacgaact
     2461 ggctccggtc gtcaaacgtg cgcgcgaaaa agtcgaacat gattttaacc agcaggtgat
     2521 caatcgagaa ctcgcaagct tgctgcaagc tttatagagg agaacgcatg ccatttaaaa
     2581 aactctcccg acgcaccttc ctgacggcaa gctcggcgct tgccttcctc catacccctt
     2641 tcgctcgcgc acttcccgcc cgacaaagcg ttaacattaa cgactacaac ccacacgact
     2701 ggatcgcctc atttaaacaa gccttcagcg aagggcaaac ggtcgtcgtg cctgctggat
     2761 tcgtttgtga caatatcaac accggcatct tcattcctcc tggcaaaacg ttacacatcc
     2821 ttggaagcct gcgcggcaac ggcagagggc gatttgtctt acaggacggc agccaggtga
     2881 caggggagga gggcggcagt atgcataaca tcaccctgga tgtgcgtggt tctgactgca
     2941 ccatcaaagg gctggcgatg agcggctttg gtccggtaac gcagatttat atcggcggca
     3001 aaaacaaacg ggtcatgcgc aacctgacca tcgataacct gaccgttagc cacgctaatt
     3061 acgccatctt acgccaggga tttcataacc agattatcgg tgccaacatc accaattgta
     3121 agttcagcga cttacagggc gacgccattg aatggaacgt ggcaattaac gacagtgata
     3181 ttttgatctc cgaccatgtc atcgagcgca tcaactgtac taacggcaaa atcaactggg
     3241 gcattggcat aggccttgcg ggaagcactt acgataataa ttacccggaa gatcaggcag
     3301 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
     3361 aaaatggcaa acattttgtt atccgtaata tcaaagcccg caatatcacg ccggatttca
     3421 gtaagaaagc gggcattgat aacgcgacag tcgctattta cggttgtgac aatttcgtga
     3481 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
     3541 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataacaccc
     3601 atcttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgctgcc tcctttgtgg
     3661 cactgactaa cattgagatg aagcgtgcct cgctggagct tcacaataaa ccgcaacatc
     3721 tttttatgcg taatatcaaa gtgatgcagg aatcctcagt tggtcccgca ttgagcatga
     3781 acttcgacat gcgcaaagac gttcgcggcg tgtttatggc gaaaaaagaa acactgctgt
     3841 ctcttgcaaa tgttcatgcg gtgaatgaaa gagggcaaag ctccgtcgat atcgacagga
     3901 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagagagt
     3961 agatttgcga ccattcataa acttactgga tataatgttc taaaagatta taattctcac
     4021 actaactgtt aaagctgggg ctgggcagca ttgactactc agggaaggta ctcatcttta
     4081 tcttgtgttt cttacgtaac aaccagacta aagtgattta tgaacgataa cgttttgctc
     4141 ataggagctt ccggattcgt aggaacccga ctacttgaaa cggcaattgc tgactttaat
     4201 atcaagaacc tggacaaaca gcagagccac ttttatccag aaatcacaca gattggcgat
     4261 gttcgtgatc aacaggcact cgaccaggcg ttagccggtt ttgacactgt tgtactactg
     4321 gcagcggaac accgcgatga cgtcagccct acttctctct attatgatgt caacgttcag
     4381 ggtacccgca atgtgttggc tgccatggaa aaaaatggcg ttaaaaatat catctttacc
     4441 agttccgttg ctgtttatgg tttgaacaaa cacaaccctg acgaaaacca tccacacgac
     4501 cctttcaacc actacggcaa aagtaagtgg caggcagagg aagtgctgcg tgaatggtat
     4561 aacaaatcac caacagaacg ttcattaacc atcatccgtc ctaccgttat cttcggtgaa
     4621 cgcaaccgcg gtaacgtcta taacttgctg aaacagatcg ctggcggcaa gtttatgatg
     4681 gtgggtgcag ggactaacta taagtccatg gcttatgttg gaaacattgt tgagtttatc
     4741 aagtacaaac tgaagaatgt tgccgcaggt tatgaggttt ataactacgt tgataagcca
     4801 gacctgaaca tgaaccagtt ggttgctgaa gttgaacaaa gcctgaacaa aaagatccct
     4861 tctatgcact tgccttaccc actaggaatg ctgggtggat attgttttga tatccttagc
     4921 aaaattacgg gcaaaaaata cgctgtcagc tcagtgcgcg tgaaaaaatt ctgcgcaaca
     4981 acacagtttg acgcaacgaa agtgcattct tcaggttttg tggcaccgta tacgctgtcg
     5041 caaggtctgg atcgaactct gcagtatgaa ttcgttcatg ccaaaaaaga cgacataacg
     5101 tttgtttctg agtaatgcag acgagtcaga gaaaaaagga ccaggttgga ctacgattat
     5161 tcggaaagag gtactgatta aattatcagt atgttgattt ccgagtaatc cgagtaaccc
     5221 agatgctgat ggtgtgtagt gttatcgtaa ggtttaatca aagttatgcg gaactaagct
     5281 ttggtatata gccataacgt ttggctaacc tttggcaata attcacgcac tatcagccac
     5341 ttagaggtag gaaaagaatg acgaatttaa aagcagttat accggtagcg gggcttggga
     5401 tgcatatgtt gcctgccacc aaggcgattc ctaaagagat gctaccgatc gtcgacaagc
     5461 ctatgattca gtacattgtt gacgagattg tggctgcagg gatcaaagaa atcctcctgg
     5521 taacgcatgc gtccaagaac gcggtcgaaa accacttcga cacttcttat gaattagaat
     5581 cactccttga acagcgtgtg aagcgccagc tgctggcgga agtacagtcc atctgcccgc
     5641 cgggcgtgac aattatgaac gtgcgtcagg gtgaaccttt gggattaggt cactccattt
     5701 tgtgtgcacg acccgccatt ggtgacaatc cgtttgtcgt ggtgctgcca gacgttgtga
     5761 tcgacgacgc cagcgctgat ccgctgcgct acaaccttgc tgccatgatt gcgcgcttca
     5821 atgaaacggg ccgtagccag gtgctggcaa aacgtatgcc gggtgacctc tctgaatact
     5881 ctgtcatcca gaccaaagag ccgctggacc gcgaaggtaa agtcagccgc attgttgaat
     5941 tcatcgaaaa accggatcag ccgcagacgc tggactcaga catcatggcc gttggtcgct
     6001 atgtgctttc tgccgatatt tggccggaac tagaacgtac tcagcctggt gcatggggac
     6061 gtattcagct gactgatgcc attgccgagc tggcgaaaaa acagtccgtt gatgcaatgc
     6121 tgatgaccgg cgacagctac gactgcggta aaaaaatggg ctatatgcag gcgtttgtga
     6181 agtatgggct gcgcaacctg aaagaaggta ttaagtttag agaaaaaatt ttaaagttac
     6241 tttaattttc atcttatcta cgg
//
LOCUS       DAZGCQ010000204         6258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000204 DAZGCQ010000000
VERSION     DAZGCQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.204"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..425
                     /locus_tag="ACHK07_004965"
     CDS             210..425
                     /locus_tag="ACHK07_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8105174.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            599..856
                     /locus_tag="ACHK07_004966"
     CDS             599..856
                     /locus_tag="ACHK07_004966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105175.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMRTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            838..1029
                     /locus_tag="ACHK07_004967"
     CDS             838..1029
                     /locus_tag="ACHK07_004967"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8105176.1"
                     /translation="MCKRLLAIPGFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1171..2190
                     /locus_tag="ACHK07_004968"
     CDS             1171..2190
                     /locus_tag="ACHK07_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8105177.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWVRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2947..3126
                     /locus_tag="ACHK07_004969"
                     /pseudo
     CDS             2947..3126
                     /locus_tag="ACHK07_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3301..5271
                     /locus_tag="ACHK07_004970"
     CDS             3301..5271
                     /locus_tag="ACHK07_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO8105178.1"
                     /translation="MYMNVIRTVICTLIILPVGLQTATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLEPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNVASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYAPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5290..6081
                     /locus_tag="ACHK07_004971"
     CDS             5290..6081
                     /locus_tag="ACHK07_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO8105179.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1858 a         1386 c         1513 g         1501 t
ORIGIN      
        1 ttatttagaa ggtctgtctt ctttttaata ttaacttatt aattggtgct attcatataa
       61 ttctttaaac agcgaactta tagccaggat tcaatagata tagtaacagg aacatcagta
      121 agcgatgaga tggtcttccc cctccttcac gacaaccttt aattacgtca tgatggataa
      181 actttaacca caaaagtaag agggatacca tgtctcaaca gaaaatcacc ggcatcgatc
      241 tggctaaaac gaacttttat cttttcagta tcaatgctca tggcaaaccc gcaggaagga
      301 tcaaactttc tcgcaatcaa ctgcttaact ggctggtgca acagcccaaa atgactgttg
      361 cgatggaggc tcgcgggcat cccatcactg ggccagggaa atcaggaaac tcgaccatga
      421 tgtaatattt cttccggcgc aacatgttaa agcttatcag cgacgccaga aaaatgatta
      481 tgacgacgct caggcaatag cagaggcctg ccaacatgga actatacgcc ccgtaccaat
      541 aaaaactctg gagtaacagg acgtccagac ttttttgaaa atgcgccggt tggtttcgat
      601 ggaaaggaca caactgataa atcacgttcg tggacttctt gctgaatatg gcattgtgtt
      661 cagtaaaggc gcaacagaat tacgccaaaa attaccggca ttacttgagg atgcggaaaa
      721 cgaactcact gataccatga ggacgttatt gcatcggcag tatatacggt tgattacact
      781 agataatgaa ctggagtggt atgattctga actgaaaaat atgtccgcca agatcctatg
      841 tgcaaacgct tgttagccat tcctggattc agccctctgg ttagtcaggc aataaaaagc
      901 tggatggggg atggaaaaca gtttagacga ggaagagatg catctgcgac tctgggactg
      961 gttccccgac agttcagcac aggtgggaag caggttcttc tgggtataac aaaatttgga
     1021 gaaatgtaat gaacacatcc caccccatta agtcaggtgc tctgtgatgg atgaagggaa
     1081 atactggaag gtgtggagcc tcgcagattt ccggcatcat agtgcccaat acaggatgca
     1141 taaagctccc tcagactgga gactccacaa atgaaatata caccggttgg cgttgatatc
     1201 gcaaaacatc tgattcaggt tcatttcatc gacgagcaca caggtgaagt gattgataag
     1261 caactacgtc gtcaggattt tctgatgttc ttctgtaatc gggaaccctg cctgattggt
     1321 atggaggcct gtagaggctc tcagcactgg gtgcgtgaac tgacaaagct ggggcataaa
     1381 gtccggttgt tacaggccca ctttgttaag gcattcgtca tgggaaataa gaatgatgtg
     1441 atggatgccc gggctatctg gatggctgtt cagcagccag gtaaggacat cgcggttaaa
     1501 acagaagaac aacagtcagt gctggttttg caccgttccc gcagacaatt ggttaagttc
     1561 cgcaccatgc agattaattc tctgcacggc acgctcctag aatttggtaa aaccattcac
     1621 aaaggtcggg cagcgatgga gcgagagctc cctgaagcac tggagcggat gaaagagaga
     1681 ctgccgccgt atctcattac agtgctggaa aaccagtaca accggctgaa tgagctggac
     1741 tcgctgatag aggatattga aaaaaagctt accagcgtgg cgagacagaa tgaaacctgt
     1801 aagcggttgc tggatattcc cggcgttggg ccacttattg caacggcagc ggtggccacc
     1861 atgggggaag catcagcgtt taaatcaggg cgagagttcg cggcttatgt tggtctggtg
     1921 cccaaacaga ctggctcagg agggaaaatc cgcctgctgg ggataagcaa acgtggtaat
     1981 acatatctgc gagccttgtt tattcatagc gcaagggcgg cagcattact gcccaaagaa
     2041 cctggtccgt ggataaccga actgaaaaaa cgtcgtccaa ccagcgtggc aatcgtcgcc
     2101 atggcaaaca aactggcacg aacagtatgg gcgatagcag ctcatggaag taaatacgac
     2161 aagaatcatg tcagtatcag atcatattaa tctctgatac cgataaatat taacttttaa
     2221 tgaaaggatg aatactgaaa ggtaactctg gcggccatag tgatgacaac gacaggtaag
     2281 accgggactc actaaacctg aatattgtgt tgagctaaaa gctcgctgga gaaatgaggt
     2341 gtgagtcagc gaattacata ggggctcgca gcttcatggc tgcaaaaaag ccggatataa
     2401 agctgcaacc tacccgtcac tgtcaaggca atggatgcct tgcaacggga tgtgttcata
     2461 taatcaatat gtcagatcta tgctgataca gggtgccaga gcggtattat tcagagcctc
     2521 agagaaaact gattcacttc gtgtgtgggt taattgtatt cgggaaaagc gtggcttcaa
     2581 ccgggcagtt gttgctcttg caaataaact ccttcggatc gcctgggcaa ttatcgcccg
     2641 caatgagatg tattttcccg cagcaaaaca aaatcaataa tctacgtaaa atcagtagga
     2701 gtaaagctta ccctgaaatt gcaaagaatg cagaatgatg aaaaacaggt cagaccgacg
     2761 ttacgaaaac ctgcaagatg agccaggcca gtaaggccga gtaagcgata aggtcggagc
     2821 gcgcgaatgt ctcatcaagg ccagaagcaa taacttcatg aacaaaccgg atatacgaaa
     2881 gtaaacctgt ccctatcata gttgctgtac tcttgcaata cgggggaaga tcatctcatc
     2941 acttacgaac gattcgtgaa aacgatgaag aaagactaca tcgcgtgcat gccgaaatct
     3001 aatgtaagaa cggcattgca taatcttgca gtggagatcg ggtattacaa tgaaaaccat
     3061 ccgcatagtg cgttaggtta tctctcacca agggaatatc gacgtcagca agtaacgtca
     3121 ccttagatgc agtagttgtc aggaaatagt gactcaaggg caggcttacc atatacactc
     3181 ttagtggttc gactgggtat tagaatagct attaacagat tagcactgaa cactgcgtaa
     3241 tagtggttgc acaaacaaaa tgttatgtta taacattgct gtattgacat atattttcga
     3301 atgtatatga acgtaatcag aactgtcatt tgtacattaa tcatacttcc ggtggggcta
     3361 cagacagcga caagtcattc ttctatggct aaagatacaa tcaccgttgt cgcgacagga
     3421 aatcagaaca cggtatttga aacgccgtcg atggtcagtg tcatcacgaa tgacacgcca
     3481 tggagtcaga atgcggtcac atcagccggt atgctgaaag gtgttgcggg tctcagccag
     3541 actggtgcag gacggaccaa tgggcagacc tttaatctac gcggctatga caaaagcggg
     3601 gtacttgttc ttgttgacgg cgtccgtcaa ctcagtgaca tggcaaaaag cagtggcact
     3661 tatctcgagc cggcactcat caaacgcatc gaagttgtcc gcggaccaaa ctccagtctg
     3721 tacggcagtg gcgggctggg gggggtggtg gacttcagaa ctgccgatgc tgcagatttt
     3781 cttccccccg gagagacaaa cggtgtaagt ctgtggggaa atgtcgccag tggtgaccac
     3841 agcacaggct cggggctcac ctggttcggt aaaaccggaa aaacagatgc gcttctttct
     3901 gtcattatgc gtaaaagagg aaatatctat cagagtgatg gtgagcgtgc acctaacaag
     3961 gaaaaacctg cagccctgtt tgcgaaaggc tctgtcagta taacagacag taacaaagca
     4021 ggtgccagct tgcgtctcta ccagaataac accactgaac cgggcaatcc cactctgaca
     4081 catggtgaca acggcctgcg tgacagaaaa acagcacaaa atgacgtgca gttctggtac
     4141 cagtacgccc ctgcggataa cagcctcatc aatgtaaagt caacgttata tctcagtgat
     4201 atcactatca agacaaacgg tcacaacaaa acagcagaat ggcgaaacaa cagaacctcc
     4261 ggtgttaatg ttgtcaacag gagccatact ctgatttttc cgggagccca tcagttaagt
     4321 tatggcgctg aatattaccg tcagcagcag aagccggaag gctctgccac actatatccg
     4381 gaaggaaaca ttgactttac atcgttgtat ttccaggatg aaatgacaat gaaaagcaac
     4441 ccggttaaca ttatcgtcgg ttcccgttat gaccggtaca agagcttcaa tccccgagcc
     4501 ggagaactga aagccgaacg cctgtccccc agggcggcga tttcagtctc accgacagac
     4561 tggctgatga tgtacggctc catatcctct gcattccgtg cccccacaat ggcagaaatg
     4621 tacagggatg atgtacattt ttaccgcaag ggtaaaccca attactgggt acctaacctt
     4681 aatctgaaac cagaaaataa catcacccgt gagattggcg caggtattca actggacggc
     4741 ctgcttacag acaatgatcg gctgcagttg aaaggcggat atttcggaac ggatgccaga
     4801 aactatattg ccacgcgcgt ggatatgaaa cggatgcgtt cttattctta taatgtatcc
     4861 cgggcccgta tctggggatg ggatatgcag ggtaattacc agtctgatta ttttgactgg
     4921 atgctttctt ataaccgcac ggaaagtatg gatgccagta gcagggaatg gctgggctcc
     4981 ggcaatcctg acacactcat cagtgacatc agtatacctg ttggtcatgg aggagtttct
     5041 gccggatggc gcgctgaact ttcggcagca gccacgcatg tgaaaaaagg cgatccccat
     5101 caggctggct atgccgtaca ttccttttca ctgtcttata agcctgtaag tgttaaaggc
     5161 tttgaggcat cagtgactct ggataatgcc ttcaacaagc ttgccatgaa tggcaaaggt
     5221 gtgccgcttt caggcagaac tgtcagtctt tatacccgtt atcagtggta acctcgcagg
     5281 aaataaatca tgatgaaaaa tacaggctat atcttagctc tttgtctgac agcatcggag
     5341 catgccctgg cccacgatgt ctggattacc ggtaaacagg cagagaacaa cgttaccgca
     5401 gagattggtt atggtcataa tttcccctca aagggcacaa ttcctgacag aaggaatttc
     5461 tttgaaaatc cccggcttta taacgggaaa gagataataa cgctgaagcc aacgtccacg
     5521 gattatgtct ataaaactga gtctgcaggc aacggtaata gctacgttct gtcaacgaaa
     5581 atgaaaccgg gatactggtc gagaacctcg tcaggatgga agcctgtcag tcgggagggc
     5641 agaaatgatg tggcatactg tgagtttgtg actaaatatg caaaatcttt tattcctggt
     5701 gaacagcaga tgccagcaca actctatcag tctccaacag gggatgagct tgaaatcatt
     5761 ccattatccg atatcagtcg tttcggtgaa gatgtgaagc tgaaagttct gtataaaaca
     5821 tccccgcttg ccggagctac tctggagctt gactctgtca gttatctgaa atcatcccgt
     5881 catacccatg cagctgagca taaacattcg gctcataaag cagaacttac ctttgtaagt
     5941 aatgaggacg gtatcatcac tgtaccttcc cttcatgtcg ggcaatggct ggctaaagtc
     6001 aaaaataaga aagtatttcc ggacaagagt ctgtgtgatg aaactgtcga tgtggcaacc
     6061 ttaagcttct cccgaaatta ataatactat tcctgccctg atttatgggc agggtattat
     6121 caatacgaga atagcctacc gacatccgtg taaaaaacca cataatgatt tttcatatcc
     6181 atttccagat atacttatgt aaagatcacc tccaaaaaag cgtaatagta ataaaaatcc
     6241 ctgaagcacc ctgttcaa
//
LOCUS       DAZGCQ010000205         6211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000205 DAZGCQ010000000
VERSION     DAZGCQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.205"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(128..445)
                     /locus_tag="ACHK07_004972"
     CDS             complement(128..445)
                     /locus_tag="ACHK07_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HFO8105180.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVIGGAFGAGLC
                     GPVCAGAFAVGSSAAVAALYDAAGNSNSAKQKPEGLPPEAWNYAEGRMCNWSPNNLSD
                     VCL"
     gene            complement(662..898)
                     /gene="cvi"
                     /locus_tag="ACHK07_004973"
     CDS             complement(662..898)
                     /gene="cvi"
                     /locus_tag="ACHK07_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HFO8105181.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFREKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            1490..1636
                     /locus_tag="ACHK07_004974"
                     /pseudo
     CDS             1490..1636
                     /locus_tag="ACHK07_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(1686..2039)
                     /gene="cmi"
                     /locus_tag="ACHK07_004975"
     CDS             complement(1686..2039)
                     /gene="cmi"
                     /locus_tag="ACHK07_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO8105182.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQFSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(2217..2999)
                     /locus_tag="ACHK07_004976"
     CDS             complement(2217..2999)
                     /locus_tag="ACHK07_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8105183.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRQA"
     gene            complement(3001..3414)
                     /locus_tag="ACHK07_004977"
     CDS             complement(3001..3414)
                     /locus_tag="ACHK07_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105184.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3983..4240
                     /locus_tag="ACHK07_004978"
     CDS             3983..4240
                     /locus_tag="ACHK07_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105185.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            4240..4830
                     /locus_tag="ACHK07_004979"
     CDS             4240..4830
                     /locus_tag="ACHK07_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105186.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDSGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNREG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(4848..5195)
                     /locus_tag="ACHK07_004980"
     CDS             complement(4848..5195)
                     /locus_tag="ACHK07_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO8105187.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLSMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            5314..5514
                     /locus_tag="ACHK07_004981"
     CDS             5314..5514
                     /locus_tag="ACHK07_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105188.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            5554..5736
                     /locus_tag="ACHK07_004982"
     CDS             5554..5736
                     /locus_tag="ACHK07_004982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105189.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEAKFFNPFPPR
                     AFENIPLYTEEITKYD"
     gene            5729..>6211
                     /locus_tag="ACHK07_004983"
     CDS             5729..>6211
                     /locus_tag="ACHK07_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8105190.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFFWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFSVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVSGVLS
                     A"
BASE COUNT         1687 a         1508 c         1377 g         1639 t
ORIGIN      
        1 taattcctcg ttatgttcag ttgtcagttg gaggaggaat ggaagataga caacccacca
       61 ggaagcatat gtatcattct gtgtggattg tccaataaca gttctttcct atattaaaaa
      121 atactattta taaacaaaca tcactaagat tatttggact ccaattacac attctacctt
      181 cagcgtagtt ccatgcttct ggaggtagtc cttctggttt ttgtttcgct gagttggaat
      241 ttcctgctgc atcatacaaa gcagcaacag cggcagaaga tccaacagcg aaggcaccag
      301 cacaaacagg gccgcacaga ccagctccga atgcaccgcc aatcactcct gcaccgtttg
      361 ttgctacagt tttcccgaca tcaacccaat tgacatcacc agcaccagag acattattca
      421 tttcatttaa cgttatttct ctcatatatc atttacccct attaaaaaaa atagatatca
      481 ttaatgcaat ggcgataccg ctaacaacag attgaacaag gttgctagaa tttagttgtt
      541 tagcaataag actaataata atgcttatgg gtaatataat aaaaaatact tgccacgttt
      601 tcatctatat tcctgcgcta aagttatagt aacgccaaca cctccagaaa cagaatctaa
      661 ttcatttaga gtcagagttc tcatatgatc tcctttttat cttatcggat attgaataat
      721 aattatcacc aacaaagtaa catattgcag acattaatgc agagaagcaa aatgtatgca
      781 tggataaaaa atccttttct ctaaaaacac aatcatatat agctaatgca atatatattg
      841 cggtggcatt tattataaat gcaaataaca actctaattt tgttcttttt ctatccatta
      901 ctttctatcc cattaccata caaacactaa cgataatgat tatcgtttac atagtcaaga
      961 gtgaagggta ggggagccct caccccccta taaggggtcc gcctggaaaa cggagtttcc
     1021 cacgtcaaga gaattgagtt gaacaagtgg ttacatccat ccaagccagc taactcacta
     1081 aacgtaaagg atgtacgaaa tgttcctacc gtccgataac catttggctt acttccgtac
     1141 attcccttgc gtagatgttc agaattgtac gagttcggac accaaaaccg ctgttgtccc
     1201 gccattctgg cgattcctga aagtcatttg actaaaaaat aggggctatt tcgaattttc
     1261 tggacactcc tgacgcggag tctgtgactt caacctgaca ccttacgctc ttagcgtacg
     1321 attttttccg tttcgtgagt tgaccccttt attttccaaa ttccaaactg ccccctacat
     1381 ttatacaatg tccggcaggg catagaaggg gagaaagaaa atacctgggc gaagccgctg
     1441 aacatcattc tggcgatttc aataaaggca tttgctcgcg ctctgtcatt atgatgataa
     1501 atacgatttt aacgccagca ctcaccgtgg cgtcatcgga gagtcgctca caaggctcgg
     1561 ggcgatgtgt tctggtaaag agtaccagat actgcttcct ggtgaaattc acattaaaga
     1621 aagtggtaag cgataatata agggccggga ttccggcctt tttatgcagc ctttataaca
     1681 gtgtattagt cattcgcata ttcaacatat ccctcctcac aatggtacat gccataatac
     1741 attctcttac cctccggacc ttcagcttta aatgggacaa gaagtgtttt ttcatcttca
     1801 acatctgtta aacttctttc aatcttttcc catgtaattt tcgggatttc tgtaccaaga
     1861 agttgtacat cgttgtccca gcgtttcatt ctttgcaggt aatcattttt tacaaatgtt
     1921 ctggcctgtt catcgctgaa ctgatagcat gccggacctt tatcttcaga ccagaaaaaa
     1981 acagcagcaa gagcaataat cgttaaaata aaaataaatt tcatgctaat tactttcata
     2041 aaatctccac aacttctgtc gttcattcga tataaaaaac attacgaata tatccgtaca
     2101 atgtgagcat actgtgaagt ggctttaaca attatgaaat aatacaagag atcacatatt
     2161 gcatgtggtc tcttttttta cagaataaat cacaattcat aattatgact tatgatttat
     2221 gcctgcctgt ttttcagcat tgtgacagca tcggactcag gcatcaaaaa ctgcacccgg
     2281 tgccgtgcag ccacatccag tgcaaacacc ttcgtgtaga cctccgttga gctgatggac
     2341 ttatgcccca tcaggctctg caggaccttc agcggtatac cggcatacag catgtgcatc
     2401 gcataggaat ggcggaacgt atgtggcgtg accgggacag agaacgtcac accatcagcg
     2461 gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
     2521 atccgcgctt tctctgtcct gcctgttcgt ttattacgcc gttccatggg gattttcagt
     2581 gtggccacca tcgtctgcag ctggctgacg taccaggtgt cggaaagcgg aaccagccgg
     2641 tgagtctgct gaccggcggg cattcttcct gccgtcctgg cggctttttc tgtccgctgc
     2701 ttcagagtgg ccagctgcac aaacggatac ggaggcgtga gcgaaaaatc cccccgcgtc
     2761 agcgccagtg cttcattaat gcgcgcaccg gtgttccaca gtgtggccag caacatcttg
     2821 cggcgcagat ccgggacgta atggagcagg gcgctcactt caggggccag caggtatttt
     2881 ggcagttcat catgaaccat cgacatctgg cggagtgcca gcgctgccgg ataatcgatg
     2941 gcaacaggaa gctgagcggc atcccgggca tggtggatcg gtgccggcag gtgctgcata
     3001 tcagtgttca ctccacgggg aaacgtcggg caggttttcc acaacacggt actttcccgg
     3061 ataacggagc tggttaccgc cacgcgtcca gacggtgaca acaaaacccc ctccctggtt
     3121 gttttcgggg agcaggcgat cgcactgctc actcagcatg tggatcacaa acggcgcatc
     3181 gctgccgtct tcaaatacca gttccagcgc atcgcggcca tgcagcgttc ccttactgat
     3241 gatgacgtac tcagcccccc gcatctccct gagtaaatgt tttgccgcat ccgggacaag
     3301 cagcctggct gcgccggcat tccagctgag gtagacatat ccggcctgcg cctgtacaga
     3361 ctgccagtaa tccgttccgg cgacagcctg accttcattc tcaataaaaa acatgggtga
     3421 ctcctttttt cttttccggg cagggacggt catgcgataa cgtcatccat catcactgcc
     3481 cttaccgtca cgccgtctga taacatcatg ccgttatcat catcaccatc ataacaagca
     3541 tccatataac cacgccgtta aattaccgga gagccgtttc cgtcagatcc gactttccgg
     3601 aatatcacaa ctttccggaa gtgtaccacg ctgaggaaat aatcatatcg ctgttttccg
     3661 cccctgcgcg gcgcatcaca ggtcagggag atgacacaac atatagtgtc atcagcagct
     3721 gaattcagca ctacatgttg tgctgacagg aggtggcgtt gtgtgggggg agaggaagat
     3781 cctgaacagc cggaaaccgg atctgcgcgg cgggaaacgg gcaggcgata tgataattaa
     3841 gcaacacaca taaattcata tcagatatga cttcaatcat aagtaatgaa acgatatctg
     3901 atattatcat aaatcgataa cacaaaaatc atatagaatt tgatttttac ctgtcagaac
     3961 attatcagat ggagcgcgca ggatggatga agaaagggtg ttttcactca gttatgaaca
     4021 actgacccgg tttgcggaaa aacgcatccg ggaatgcaat ctggacagcc agggcgcgat
     4081 ttatctctgc gagtcagcca aagctggagc cgtgctgatc ttctggcatg aactggccat
     4141 aaatggctat gcctcaatga atgccataaa gagacaggag ctcattgacg ccgactttca
     4201 gcgcctcaga aacctcatct ggccggagga tgacaggtaa tgacccagag ccgccgaccg
     4261 tcgccactgc agcggagagt gctgattgtg ctggctgccc ttgatgaaaa acggccaggc
     4321 cccgtgctga cgcgggacat agaacgggtg ctggagcgaa gcggggaagc cccggtctac
     4381 gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatt ccggctggct gcgtaccctg
     4441 cgcgctccga acctgcaact ggcagtggag ctgacggacg ccggccgggc cgtggcacag
     4501 cctctgctgc tggcagaaca ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc
     4561 gtactgccgc tgatcccggc agcaggtctg cccgcagacg gcacttctgc cactgatctg
     4621 gcggtgcagc tgaacggcat aacgtatcag gcctgccgtg gggattttgt ggtacgtctc
     4681 gacggcagca cctgtctgca gttatggaac agagaaggca gggtggtacg ccgggagggc
     4741 gatccgctgg aagtggcgca gtggctgcag gcctgtcacg atgcaggaat ggaggttcgt
     4801 gtgcaggtta atgaaagcat caccccgtaa aaacatgcct gcacggttta cacatcatcc
     4861 catggtggca gccggttaac tttcctgcgc caccagtgga aagaggccgt gagcagtccg
     4921 aacaaaaaac cacccgtgat acttataata atggcctcac ctgccaccat tcctgacggc
     4981 ccccagtaga taaaccacat tgcacatccc caggaaatgc cccataagct ccccatcgac
     5041 agcgtgacct gccagaacgg cataaagggt aatggcggaa gccggatacc cagccgccag
     5101 agaatacgca gaagaggagg ggcataattg ctccgccaca tctttttgct gtccatcagg
     5161 gcaatggccc gggctttttt ctgctcaaag gtcacaggag cacctccgtt ccatgatgta
     5221 aacgtgacac agcttattgt tattgattat tattatcata ctgtgattat gttgtcatgc
     5281 accggtacac gataacaata aaggagaaac cagatgaaac tggatatatc tgtaaagtat
     5341 tttcttaata ttatgatagc agatttcatc attcccgcac cagaaaaaat atcaaccaca
     5401 tggcactcag atactaaaat gaatcgcttt gcttgcccta tacattctac taatagtgat
     5461 tactattcag gtttttatca tacagatgta ttaattcctg gagaactggc ttaggacgcc
     5521 atcataatcc atgatgttga tagtaatgat cctatgattt ttaactggaa tgtattgttt
     5581 tatgtctcac tggaaaagtt tcctgttggc aatccttatt atggagaaat tccaggtatt
     5641 cctgtggatg aagaagcaaa gttttttaat ccatttccac caagagcgtt tgaaaatata
     5701 cctctttata ctgaggagat aactaaatat gattaaggga attatgtttg catttcttgc
     5761 tgctttttca tggggagcag caatagtgat gagcaaaaaa ggtctcgaaa acatggatgc
     5821 cggagagttg tttttctggc aagttggtag tgctgcaatg ttatcatggt ttgttctcgc
     5881 aattagcaga aaaaaactcc cggtaacaaa aaaatcaatc cttgcctatt caacaggaat
     5941 ttttgagcca tttctggcgt atacattcac tctctatggc ctaaaattta tttctgctgg
     6001 tataacagct gttatttttt cacttgagtc agtatttatt cttatactat ctgttatatt
     6061 attttccgtt aaaattaact caccattaca ctttatatta ttgcttactg gagcaatggc
     6121 aggcagtata atggttgccc tacctgatat caatgaccgc attgataata ttacaggata
     6181 tatacttatt gtttcaggtg tactctcagc a
//
LOCUS       DAZGCQ010000206         6112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000206 DAZGCQ010000000
VERSION     DAZGCQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.206"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..534
                     /locus_tag="ACHK07_004984"
     CDS             <1..534
                     /locus_tag="ACHK07_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105191.1"
                     /translation="PGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKD
                     MDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLMVEKGDKITGVRKGSLSENVFQ
                     DRGTIAGSVHVDAVDNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVI
                     RLSKDGITIVGGSVFIN"
     gene            562..1023
                     /locus_tag="ACHK07_004985"
     CDS             562..1023
                     /locus_tag="ACHK07_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO8105192.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1043..5920
                     /locus_tag="ACHK07_004986"
                     /pseudo
     CDS             1043..5920
                     /locus_tag="ACHK07_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            5938..>6112
                     /locus_tag="ACHK07_004987"
     CDS             5938..>6112
                     /locus_tag="ACHK07_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105193.1"
                     /translation="MKEIDLLYENIYQLLIKPYLLDLSSQSGKKIEVNYTCKIKDAAD
                     EIKGSMIFNDVDGK"
BASE COUNT         1556 a         1583 c         1835 g         1138 t
ORIGIN      
        1 cccggcagcc tgccggggac gaagacgcag atgaccatcc gttccaaaac gtatatgggc
       61 agcgggttta atgagctgaa gtttgacgat gcgacaggga aagaacaggt ctacatccac
      121 gcgcagaagg acatggatac cgaggtactc aacgatcgta ccaccaccgt aaaacacgat
      181 catcgcgaaa ccgtaaaaaa tgaccagacg gtcacgatac aggaaggtaa tcgccttctt
      241 atggtggaaa aaggcgacaa gatcacagga gtacgaaaag gttctttatc tgagaatgtc
      301 tttcaggaca gaggcacgat tgccggttcg gtgcatgttg acgctgtaga caatggtggc
      361 gaaggcaacg gtatacaggc ttatacggca attaaggaaa ttatgctggc cgtggaggaa
      421 agcaaaattg cgctgacgcc ggatggcatt cagctacagg tcggggaatc aacggtaatc
      481 aggctgtcga aggatggcat caccatcgtg ggcggttctg tttttatcaa ctgattcact
      541 cagattatca gggagtattt tatgcagttc acttttaatg aaggccacat tcaactgccg
      601 tctcaatggc aggatcagtc aatgcaggtc ctggtgtcca cagataacag tggcatcaac
      661 ctggtcatta cccgcgaagc cgttccacag ggaaccctga caccagagct gtaccaggaa
      721 acgctggcgc tgtaccaggg caaacttgat gggtacaccg aacatgcctg ccgggaaatc
      781 actctggcag aggccccggc ctggctgctg gattattcct ggaacggtcc ggaagatgaa
      841 gggaatcagg ggcggattag ccagatagcc gtattccagc ggcgtggaga tacgctgctg
      901 accttcactt tcagcacctc cttatccctg aagaacagtc aaaagacgat gctgctggag
      961 gtgataaaga gcttcacccc gttgccaccg gagaatgaca tccaaaagga ccagccgcgc
     1021 tgacataaac gagggataac acatgagtga aggaccaggc gggccacagg gagcgaccgc
     1081 aggcggtacg ctggcaatgc gaatgctgtc acagcaggcg atggtcgcca gccagatgaa
     1141 acgggcagcc aacgacaaag ccattgcaca gatgttggca tcaaaaaagt ctggctcccc
     1201 cgccgccagg ctgggcgatg aaattcagca caagagtttt ttgggggcgc tggcaggggc
     1261 cgtgctgggg gcgatagtga ccatcgcaga aggttgcctg attatggccg cctgtgccac
     1321 cggcccttat gcgctggttc tggtgcctgc gctgatgtat gccagctata aggcgagtga
     1381 ttatgtggag gagaaacaga accagcttga atcatggata aacagctttt gtgacacgga
     1441 cggcgccatc aataccggtt ctgaaaacgt aaaaattaac ggaaagccag cagcccgtgc
     1501 agccgtcacc cttccccctc ctcccccacc tggagcaata cctgaagtcc cacaggggga
     1561 accctcatgg ggtgatattg ccactgacct gcttgaatcg gcagcggaaa aagcagtacc
     1621 actggcgaag gcctggggga acgctgttat caccctgacg gacagcaatg ccagttttat
     1681 ggatcgcgtc agtgcaggcg catcgcttct gtttcccagc ggcccggtat taatggaatt
     1741 tgccacgatg gtgggcgggc gtggcgaaat caaaaaagat gtggatttcc cggaagccgg
     1801 tgaggacacg gcgctctgcg acaaggagaa caaaccaccg aggatagccc agggcagcag
     1861 caacgtcttt atcaacaatc agcctgccgc gcgccagggg gacaaactgg agtgcagtgc
     1921 ggcgatcgtg gaaggttcgc cggacgtctt tattgggggt gagcaggtca cctatctgga
     1981 tatccagccg gagttcccgc catggcagag aatgatcctg ggaggaataa cgatagccag
     2041 ctatcttctg ccgccagcag gactgctggg aaaactgggg aatctggcga aactgggcaa
     2101 actgggaaac ctgctgggga aaagcggaaa gctgctgggc gcaaaactcg gcgcgttgct
     2161 ggggaaaaca ggtaagtcgt taaaaagtat tgccaataaa gtcatcagat gggtaacaga
     2221 tcctgtcgat ccggtaaccg gcgcgtactg cgacgaacgt accgacttca ccctgggcca
     2281 gaccctcccc ctctccttca cccgtttcca cagttcggta ctgccactgc atggcctgac
     2341 gggcgtgggc tggagtgact cctggagcga atacgcctgg gtgcgtgaac agggaaaccg
     2401 ggtggatatc atcagcctgg gagccacgct gaacttcgcc ttcgacggtg aaagtgatac
     2461 ggcggttaac ccgtatcacg cccagtacat tctgcgccgc cgtgatgatt atctggagct
     2521 gttcgacagg gatgcactga gcagccgctt cttttatgac gcctttccgg gaatgcgtct
     2581 gcgccacccg gtgactgacg ataccagcga tgaccgcctg gcacacagcc ccgcagaccg
     2641 gatgtacatg ctgggcggga tgagcgacac cgccagcaac cgcatcacgt ttgagcgcga
     2701 cagccagtac cggatcacgg gtgtcagtca caccgacggg atccggctta aactgacgta
     2761 ccacgccagc ggctacctga aagccattca ccgcacggat aacggcatac agacgctggc
     2821 gacctacgaa caggatgcgc gggggcggct gacagaagcg gatgcgcggc tggactacca
     2881 cctgttttat gagtacgacg ctgcggaccg gatcatccgc tggtccgata acgaccagac
     2941 gtggagccgt ttcacctacg atgaacaggg ccggtgcgtg accgtcaccg gggcggaggg
     3001 ctattacaac gccacgctgg actatgggga cggctgcacc accgtgacgg acggcaaggg
     3061 cgctcaccgt tattactatg atcctgacgg caatattctg cgggaagaag cgccggacgg
     3121 cagcaccacc acgtatgaat gggatgaata ccatcacctg ctggcccgcc actcccctgc
     3181 cgggcgggtg gagaaatttg aatacaacgc cgcacatggt cagttaagct gttatacggc
     3241 ggcagacggc gcggagtggc agtactgcta tgatgagcgc ggcctgctca gcaacatcac
     3301 cgaccctgcc gggcagacgt ggacgcagca gtgtgatgaa cgcggcctgc cggtgagtct
     3361 ggtgtcgcca cagggcgaag agacccggct ggcatacacc gcacaggggc tgctgtcggg
     3421 aatattccgc caggatgaac ggcacctggg catagagtac gaccaccaca accggccgga
     3481 aacactcacc gacgtgatgg gccgtgaaca ccacaccgaa tacagtggtc acgacctgcc
     3541 ggtgaagatg cgcggccccg gcggttagtc agtgcggttg cagtggcagc agcaccataa
     3601 actgagcggc cttgagcggg caggaaccgg cgcagaagga ttccgctatg accgccacgg
     3661 caacctgctg gcgtacacgg acggtaacgg cgttgtctgg acaatggaat acggcccgtt
     3721 tgatttgccg gtggcgcgaa cggacggtga aggccaccgc tggcagtacc gctacgataa
     3781 agacacgctg caactgacag aagtcattaa cccgcagggc gagtcttatc tttatattct
     3841 ggacaactgt ggccgggtga cggaagaacg tgactggggc ggcgtggtct gtcgttaccg
     3901 ctatgacgcc gatggcctgt gtaccgccag ggttaacggc ctggaggaaa ctatcctcta
     3961 cagccgggac gccgcaggcc gcctggcaga aatcatcacg ccggaaggca aaacgcagta
     4021 tgcgtatgac aaatccggca ggctgacggg tatcttcagc ccggacggca catcacagcg
     4081 caccggctat gatgaacgcg ggcgggtgaa tgtcaccact cagggccgac gggccattga
     4141 ataccactac cccgacgaac acaccgtcat ccgctgtatc ctgccaccgg aagatgaacg
     4201 cgacagacac cccgatgaat ccctgctgaa aaccacgtac cgttataacg ccgccggaga
     4261 actgacggag gtcattctgc cgggggatga gacgctgacg ttcagccgtg atgaggcggg
     4321 acgtgaagtg ctccggcaca gtaaccgggg ttttgcctgt gagcagggct ggaatgcagc
     4381 cagccagctt gtcacccagc gcgccggatt tttcccggag gaaaccacat ggggcgggct
     4441 gctcccctca ctggtacggg agtaccgtta cgacagcgcg ggcaatgtgt cggctgtcac
     4501 cagccgggaa gattacggac gggaaacacg gcgggagtac cggctggacc ggaacggtca
     4561 ggtcacggcg gtgacagcct caggcaccgg gctgggctat ggcgaaggcg atgagtccta
     4621 tggctatgac agttgtggct acctgaaggc gcagtctgcg ggcaggcacc ggataagtga
     4681 agagactgac cagtatgccg gaggccaccg gctgaaacag gccggaaaca cgcagtatga
     4741 ctatgacgcc gcaggccgga tggtcagccg cacaaaacac cgtgacggct accgcccgga
     4801 aacagagcgg ttccggtggg acagccggga ccagctgacc gggtattgca gcgcacaggg
     4861 tgaacagtgg gaataccgcc acgacgccag cggcagacga acagagaaac gctgtgacag
     4921 gaagaaaatc cgcttcacgt acctgtggga tggcgacagt attgcggaaa tccgggaata
     4981 ccgcgatgat gaactgtaca gcgtacggca cctggtgttt aacggctttg agctgataag
     5041 ccagcagtgc agccgggtac ggcagccgca cccgtccgtg gccccgcagt gggtgacgcg
     5101 aacgaatcat gcggtgagcg acctgacggg ccgcccgctg atgctcttta acagtgaagg
     5161 taaaaccgtc tggcggccgg ggcagaccag cctgtggggg ctggcactca gcctgcccgc
     5221 agacaccggc tacccggacc cgcgcgggga gcgggacccg gaagcggacc ccggcctgct
     5281 gtatgcggga cagtggcagg atgcagaatc ggggctgtgc tataaccggt tccggtacta
     5341 cgagccggaa accgggatgt acctggtgag tgatccgctg gggttgcagg gaggggagca
     5401 gacttaccgg tatgtgccga atccttgtgg gtatatcgat cctttggggc tggctatatg
     5461 tcagttagcc cgctggacga aatgggggag tgagcaaagc aacatatctg atgttttgaa
     5521 ctcattaggg aatagagcac ttaaatatgc taatggtgat tggataaaat cagaggctgc
     5581 attcaataaa tacataaaca tgataaataa aagactagaa ttaacaggta gtaaatttag
     5641 agttgagatt caaccagcca taaaaaatgg agagcgagtt cctgcgacaa cgaatggacc
     5701 atttaaagta aatggtaagt ggacatccgg cactcattat acaggtggtt ccaaacgtct
     5761 agatgccggt attattgata tcacatctcc tacaaaccaa tatggattac atccagttat
     5821 tgaaggattt gatataacac ttaataaaac aaaaccatca gcagtggata tatattcaga
     5881 tgtgtttggt gggattgata ttaacgacat tcggttataa aacaataagg cggcaatatg
     5941 aaagagattg atttattata tgaaaatatt tatcagcttt taattaaacc ttacctactc
     6001 gatctttcta gccagtctgg aaaaaaaata gaagtaaatt acacatgcaa aataaaagat
     6061 gctgctgatg aaataaaggg tagtatgatt tttaatgatg tagatggtaa ac
//
LOCUS       DAZGCQ010000207         6100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000207 DAZGCQ010000000
VERSION     DAZGCQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.207"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2151)
                     /locus_tag="ACHK07_004988"
     CDS             complement(<1..2151)
                     /locus_tag="ACHK07_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8105194.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTDCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVTALTDNTQGA"
     gene            complement(2216..2815)
                     /locus_tag="ACHK07_004989"
     CDS             complement(2216..2815)
                     /locus_tag="ACHK07_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8105195.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDILGLVTSFSYAGDKNRQLSRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(2882..>6100)
                     /locus_tag="ACHK07_004990"
     CDS             complement(2882..>6100)
                     /locus_tag="ACHK07_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105196.1"
                     /translation="IAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLA
                     LGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDK
                     WNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIN"
BASE COUNT         1163 a         1829 c         1583 g         1525 t
ORIGIN      
        1 tgccccctgc gtattgtctg tcagcgcggt tacccgacct tttgaagcca gattaactga
       61 tgatactgct gttccacctg acggtaacgc cccgatctct gatgccgttg gcttatttct
      121 ggagttatag tcccttcgcc agccaggtga ataatctgtt ccgtgattaa tataggtaaa
      181 ctgggcgtta gttgttccac caccagtgga ggtggtcggt gtggtaatgc ggatcgtcat
      241 cgctgacttt atccccatta cttcaatgac agctccggcg agatgaatat taccgcagtc
      301 agtatcagta atgattttat tattgccata agaccaggaa cccttgcaca tccagtaggg
      361 atggttaaat gctccctgag aatccagcca ctcgataaac tgtgcagtcg tccagtttcc
      421 tgttgttgtg cttactgacc caccgaaggc acggcaggca ccaatatttt tcgtaaaggt
      481 gtctttgcca gggatatccg caccgttctg atctttctgc agacgtttct cagcattgtc
      541 attggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
      601 gctgcttagc tggattatcc ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc
      661 gttagccagg tcatacgcgg ccttaacggc ttttggtgtt gccgccagtg actcggaagt
      721 gctgttagtc gcactactga gctgtactat cccctttttc gtcgtgctcg catcctcaag
      781 cgccacggcg gatgcaatat cctctgcccg ttttgccgct gtctcagcgc gcgttgctgc
      841 agattccgcc gtacttttgc tctgagctgc tgccttcgca ctaccagcag cctctgtcgc
      901 cttcgtggat gccgtcgtgg cgctgccctt cgctgctgac gcctgtctgg tcgcctcatc
      961 ttttgaagca gacgccgatg atgccgatga cgccgccgaa ctggcggacg atgccgctgc
     1021 cgtttttgag gattctgcac gggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc
     1081 tgcggaagct gacctcgctg ctgccgtggc ctgctcagtg gcttcgccag ccttcgttgt
     1141 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc
     1201 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     1261 gcctgccgca gctgaggcgc tctgtcccgc tgccgtctca gaagacctgg cgttcgtctc
     1321 ggacgttttt gccgccttcg cggaatttcc tgccgccgtt gccgaggaag ctgcgctact
     1381 ggcgctcaag gatgcgttcg tttctgatga tttcgctgcc tcttttgagg ccgccgcatc
     1441 ccgggctgag gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggcggc
     1501 tgattgttct gacgctgcgg cattcgtttc tgacgttttc gccgcaccgg cactggtagc
     1561 tgccacgctt tttgaggact ctgcagcggc agcacttttt gaggcttcag tggcctttgt
     1621 tgatgccgtt cctgcgctgg aagacgctga ctgagccgac gaagcggcct gtccggctga
     1681 cgtgctggct gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc
     1741 agatgtgctg gcatcactgg ctgacttctt cgcggctgcc gtgttctgtg ccaccacgga
     1801 cgcgttacgc gccacctctt ccaccatcag ttcaaaacgg cgcagtgcct caggacgggc
     1861 atcatcctcc gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata
     1921 cacggtgatg gtcccggcat gtgacggcgg gaatccttcc accaacagaa taacgctgta
     1981 ctgaccgtac tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag
     2041 cgtgttcacc accaccgtgg tgctgttacg ttttgctttc agctggattg tgcagttctg
     2101 taccggtttt cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc
     2161 acaaaaaagc ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga
     2221 acttataacc gacacccatg atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt
     2281 cataagcaat atcaatggcc acggattcgg tcgggttaaa ctgcacgcca gctccccacg
     2341 ccagagacgt gttgctgtgg cgaccgtcat cacttccggt cagcacgtcg tgcgttttcc
     2401 ccttgttgtc agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca
     2461 cacccgccat cgcatacgcg ctgaaccatt cattcacgcg cacagacggc cccaccatca
     2521 cgctgaacca gcggttacga acggaatctt catgccagcg ggtatcgctg taacggctca
     2581 gctggcgatt cttgtctcct gcatagctga atgacgtcac cagccccagt atgtccgtaa
     2641 actcataacg gtatttcacg ttaatcccgt tcagatcatc gctgccggga acgttcgtcg
     2701 aggcatgaag ataccccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac
     2761 cggatacggc cagacaaatg gctgcggaca aaatggcggc ataaagttta cgcataatta
     2821 cctctcgctt ttctgcaata aaaaaggcgt catttctgac gcccgttatg ggttataaaa
     2881 ttcagttgat actgatacct gctgtggatt tcttcatcac cacaaccagc agatcgctga
     2941 tactggttgt tggtgtccag ttattcgctc ctgatgaaga tacggtgaat gtcagtgtca
     3001 gtgtcccctg tccggcaggc atatctataa ctgaggaaaa tacgccctga gcatccgtcg
     3061 tggactgatt aaaaatctcc tgaccattgc gggtcactct taaccggcag gttgaatacc
     3121 agtatgactg ttggttatta ctgttgaaat tctcatgctt accaccgcgg aataacactg
     3181 gcggtatcat gacctgccgg tcaaatttct gatcatcact gattcttacc gtgatggtgc
     3241 cactggcata actgttcgtg cgggggaaag acttgctgac cgttttgaca atatcacctt
     3301 caatctggtt ggctgacagt ttccccttaa tctgacagtt ctcattaatc gtgacgttgt
     3361 tgagcgtccc tgagttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
     3421 ttccgtctga tgtcagggaa aataccggag gactgccacc gctggtaatg gtgggtgccg
     3481 tcaggcgttt caggaacacg tcgttcatga atatctggtt gccctgcgcc acaaacatcg
     3541 gcgtttcatt cccgtttgcc gggtcaataa acgcgatacg gttagcggca accagaaact
     3601 ggctcagctt gccttcctcc gtatcctcca tactgaggcc aatacccgcg acataatgtt
     3661 tgccgtcttt ggtctgctca attttgacag cccacatggc attccattta tcgttggcat
     3721 ccttccactc tttcgaaaac tcatccagtc tgctggcgtt atcctccgtc agatctactt
     3781 tttccagcag ctctttaccg agatgggatt cggttatctt gcctttgaaa aaatccaggt
     3841 aaccttccgc atcatcgctc gcccgaccga cggcctccac aaatgccgat ttgccaacgg
     3901 tgttcacact gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg
     3961 ctatccagta cagccccgtg ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg
     4021 taatccgcgt ttccgagaac cagaactcaa actgtacagt cggatcataa accgcaagat
     4081 gcggcgtggc ggttatctga aaatagcccg gcgtcagctc aatccgcgat ggcgctgccg
     4141 gtgcggcaat ccggaacgat accgacgccg gatcgccctg ctgcccccag gcatttaccg
     4201 cccggactgt cagactgtaa tttcccagag ccagctgcct gaagcggtat gtggtttccg
     4261 tcgtccgggc cgtgctgacc agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa
     4321 gcataaagct cacgcccttc accaccttcg gcgtatccca gcgcgccagc acctggtatt
     4381 ccccgctgtc tgcggtgact tctgcggtca ggtgttgcac tgctggcgga gtgacaccat
     4441 tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg gcctctttct
     4501 ccggtacatg ctgcacggca gtgatggcat acgtgccgtc gtcgttctca cggatactca
     4561 cgcagcggaa caggcgctgg cgcaacgtcg gcaacttcag cccccacacg ctgtattcag
     4621 caacgccgtc gggaacacgg ctcactttca ccttcacgcc gtcggtgacg gactggacct
     4681 ccacgctgac cggattaccc tcaccgtcaa ccaggcttat cagcgtggtg ccggaggatg
     4741 gcagcgtgat ttcacggtcg agcgtcagcg tccgggtctg gctgtttacc gccagcacgc
     4801 gcccgccggt gctgataccg gcatagtcat catcacagat ttcaatgaca tcgcccggca
     4861 catggcgaag cccttctgcg cccacgctga agtccacggt ctgcgtttcc agcagttctg
     4921 ttttaatcag ccacagcccg gcgcggtgtg cctgcccccg gctggtacag ccaaaagcat
     4981 ccatcttcgt gacgttacga ccgtaacggg caatggcctg cgtatcctcc acaagctctg
     5041 tcgccgtctc ccagccgttg ttcgggtcaa tccagttcac ctcaacggca ttatggcggt
     5101 ctttcagggc gctgaagctg tagcggaacg gcgcgccatc atccggcatc accacattac
     5161 tgcggttata ggtccacacc ttatccgatg gccggtcctg cacaaacgtc agcgtctgcc
     5221 cgttccatac cggcatacag cgcatcgccg agcagaaatc actgagcaca tcccacgcct
     5281 tgcgctgcgt ggtcaggtac gcattacagg taatacgcgg ctccgtgcca ccaaagccat
     5341 ccggcaccga ctggtcgcaa ttctggccga tgacatacag cgcccattta tccacatccg
     5401 ccgcaccaag acgtttcccc atgccgtagc gcggatgggt cagcatatcc cacagacacc
     5461 aggccatgtt gttgctgtat gctggcttaa acgttccgtc ccagataccg ctgtattgcc
     5521 gcgtctgcgg gttatagttc gacggcacct gcagaatgcg tccgcgaaga tgataattac
     5581 ggctcacctg ctggctgccg aactgctccg agtccacctg cacgccgacc agtgccgtgt
     5641 tcgggtagca ctgtttcaca tcgatgattt cggtgtatga cgaccagagc gttttgttct
     5701 gcagctggtc tgtggtactg tccggcgtca tcctgcgcat ccggatatta aacgggcgcg
     5761 gcggcaggtt atccaccacc accgaggcca gatactgcga ggtggttttg cccttaatgg
     5821 tgatgtcttt ttccgtcacc cagccaccat tacgctgtat ctgaaccagc aggcgaactt
     5881 ccgacggatt ccggtccccc tttgaggtgg tttccaccag tgcctgcaca ccgaaggtaa
     5941 agcgcagacg gtcgatgttt gcagacgtga tggtccgggt gatcggcgtg tcgtatttca
     6001 cttccgtacc cagcaccgtc tcggatccgg aggattcaaa tccctccggc ggtgtctgct
     6061 cctgctcacc ggcccggaac accaccgtga caccggcgat
//
LOCUS       DAZGCQ010000208         6077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000208 DAZGCQ010000000
VERSION     DAZGCQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.208"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1296
                     /gene="tssI"
                     /locus_tag="ACHK07_004991"
     CDS             <1..1296
                     /gene="tssI"
                     /locus_tag="ACHK07_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO8105197.1"
                     /translation="DYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNF
                     ARWQMEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQA
                     VPGRRGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRV
                     RVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGNEQLYMHAQKNMDTE
                     VLNNRTTDVKVNHTETVGGNQAISVTGVQSTSVKSAQFTHVESLRQVAVQQGQLIVTG
                     GDHEILVQETLHLAAGKAIHLQCGESELVMLANGTVALSCTNFNLYAAQRGQITAAQI
                     LDLNMDGAKSGIEQPGPLTPSAISERVTAEFPPQQPENK"
     gene            1296..1724
                     /locus_tag="ACHK07_004992"
     CDS             1296..1724
                     /locus_tag="ACHK07_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO8105198.1"
                     /translation="MPDYHFNEGQITLPASGRDCTTHVLRLPELQAVLTISRDILSGT
                     MTLEEYVADQIAKIKRDARHVQIPEPQFTDMKNGKHVCQFSCQAEISGLVHYQQQHII
                     LSGQSLLAFAWTLGHPFTEQDHQTWRNILASFVPRETVTE"
     gene            1729..>6077
                     /locus_tag="ACHK07_004993"
     CDS             1729..>6077
                     /locus_tag="ACHK07_004993"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8105199.1"
                     /translation="MGTDYLAAKMEDPLEHTSVLGDFMGGLVEGVCYGVLFCASANPV
                     GLAVGLTAGFIFSDVISEIGDTVSSWFPPDVAGTITSGSKNVNVKSKPAARAAGAVPD
                     EILLALLEAEAANPPDSKGMQLVKGIVAKSIMIAKLPLLPAELGRMAGEKLAAYLKGE
                     STPSQEDEQGFWSRVWESISNPVVDGPDPALHVETRLLDTIDCHKHRPQPIQYLAEGS
                     ETVRINSQPACRNGDRSTCEAVIAHTQASAKVRIGGRSLVVKEIHTGKNMLAYYAGLI
                     AGGFLVSSVQALFCRTTAREFAGKLPCLMLGSVLGNTLGIVAGGTLSTSLSSLSSGLQ
                     ASSQTARPVHIASGVKILGGEDELDFACDGLIPLAWQRIYSSLNDNEGILGRGWCLPF
                     EVFLHQEPAVPGAQDSAQIFFHDMSGRELGLGDVPVGGAMFYVDEGFRLYHPLAGMFV
                     LESAQGQYQIFEADPLHDGRFRLIQECDRHLNGIRYYYNDRGLPVRIEDDLQAICLHL
                     DYHPQHSRLTAVYQQYRQEDTLPGVLRIRYEYNEQVQLTAVVDADGVVMRRFAWDEEH
                     GRLAMHQTADGLQAHYRWQPTSGLPGHFHVARHWLQEGERRSGEILFDYDPAARRATL
                     HYGDGRPDSLHEWDEQYNITRWQPAPDICWLYEWGDNRELLRVVDPAQCEYGFKYDER
                     GNLIQVVDPVGQEYRFDWDRDFAFPLKEEYPEGLVLRHVYNVSGDRIKTIDQAGLITR
                     YNHDASGLVESVQDARGNLYRYAWNNRGQLTRQTDCSGYTTHLYYDEYGRLAAVTDAQ
                     GNCTRQTFSAAGRLQKQIRPDGGETTCHYNARGQLAGVTDPARQSSRFDYNLRGQVIR
                     HTDPQQRQVRFEYDAGGQLIALYNQKNEAYRFSRDSLSRLEESCDLTGCREVYRYDSR
                     SHVSEITRYPAPAVSGQETFLPQVTRLAYDAAGRVQYRENGDGAVCYRYGKNTLSLER
                     FAPDDWQKKQRGYENIAAQDTLHLVTDAYGSLLEEHNPAGIFTHRYDELGNRTETQMP
                     DGRRLKRLYYGSGHLQQLNLEDNDGQQTVLAEFERNSLHQEISRTQGSLTLRTEYDAA
                     GRITARLAQMAQQRHGTPLLERRYSWDQRGFLMSRYHRTQDAQALLSPEERSGSTWYQ
                     YDCAGQILSAQQHFTVRQYCDLAGNLMEERSPAIKNNQLMQYRGWHYQYDAFGRMSRR
                     TDEQAQRQDYRYNSDNRMVEVRFADRTHSRWQRVEYDYDLLGRRTGKRLYRWHDEGEA
                     EPAPERVRFIWDGMRLRGEDSPGKATEVLYVYNGDSYEPLARVDRFTRQVIEPQDTRP
                     PKTTVYYYHTALNGLPEAMTSESGQLVYEARYELWGKETEAASAHTVLTVGQNLRFAG
                     QYFDAETGLHYNTFRFYAPECGRFTQPDPIGLRGGLNLYSYVPNPLSWIDPLGLKCWS
                     TEQKNYWKDVGAK"
BASE COUNT         1384 a         1575 c         1910 g         1208 t
ORIGIN      
        1 gactacacct ttaaacgccc cggctgggca ggacgttttg atcaggaagg ccagcaccag
       61 gattaccagc gcacgcagta tgaggtgtat gactaccccg gacgtttcaa gggcgcccac
      121 gggcagaact ttgcccgctg gcagatggag ggctggcgaa acaatgcaga aaccgcgcgg
      181 ggaatgagtc gctcgccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
      241 caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg cgagcagccg
      301 caggcggtgc cgggaaggcg gggagcaggg acggcgctgg agaaccattt tgcggtgatc
      361 ccggcagacc ggacatggcg accccagccg cggctaaaac cgctggtgga cggtccgcag
      421 agcgccgtcg tgacggggcc ggagggtgag gaaatcttct gtgatgaaca tggccgagtg
      481 cgggtgaaat tcaactggga ccgttataac ccggcagacc aggacagctc gtgctggatc
      541 cgtgtggcac aggcgtgggc aggcaccggt tttggcaacc tggcgatacc gcgtgtgggt
      601 caggaggtga tagtggactt cctcaacggc gatccggacc agccgatcat tatggggcgc
      661 acctaccacc aggaaaaccg cacacccggc agcctgccgg ggacaaagac gcagatgacc
      721 atccgttcga agacgtatat gggcagcggg tttaatgagc tgaagttcga tgatgcaacg
      781 ggtaatgagc agttgtacat gcatgcccag aagaacatgg ataccgaggt gctgaataac
      841 cggaccacgg atgtgaaggt aaaccatacc gaaacggtgg gaggcaacca ggctatctca
      901 gtgactgggg tgcagagtac cagcgttaaa tctgctcagt tcacgcatgt ggaaagttta
      961 cgtcaggttg cggtgcagca ggggcagttg atagtgaccg gcggggatca cgaaattctc
     1021 gtacaggaga cgctgcatct tgccgcagga aaagccattc atctgcaatg cggtgaatct
     1081 gagctggtca tgctggccaa cggtacggtt gccctgtcct gtaccaattt caatctttat
     1141 gccgcccaga gaggacaaat taccgcagcc cagatactgg atctgaatat ggacggcgca
     1201 aagtcaggta tcgaacagcc cggtccactg acgccgtcgg ctatttctga gcgtgtcacc
     1261 gccgagtttc ccccgcagca accggagaat aaataatgcc ggattatcac tttaatgaag
     1321 gacagattac gttaccggct tcagggcggg actgtaccac ccatgttttg cgactgccgg
     1381 aattacaggc tgtgctgacc atctcgcggg acattctgtc aggaaccatg acgctggaag
     1441 agtacgtcgc cgatcagatt gcaaaaatta agcgcgatgc acgccatgtg caaatcccgg
     1501 agccgcagtt tactgatatg aagaatggaa aacacgtctg ccagttttcc tgccaggcag
     1561 agatttccgg gctggttcat taccagcaac aacacattat cctgtccgga caatcgctgc
     1621 tggccttcgc atggacgctc ggtcatccgt ttactgagca ggaccaccag acctggcgga
     1681 atatcctggc cagttttgtc ccgcgtgaga cggtaacgga gtaatgtcat gggaacagat
     1741 tatctggcgg ccaaaatgga agatcctctt gagcacactt ctgtcctggg ggatttcatg
     1801 gggggcctgg tcgaaggggt atgctatggg gtactttttt gtgcctctgc caaccctgtt
     1861 ggactggcgg tagggctgac tgccggcttt attttcagcg atgttatttc tgaaatcggc
     1921 gatacggtca gtagctggtt tcccccggat gttgcaggta cgataacgtc aggctcaaaa
     1981 aacgttaatg tgaaatccaa gccagccgcc cgtgcggcag gtgcagtgcc ggatgaaata
     2041 ttactggcac tcctggaggc ggaagcggcc aatccaccgg acagtaaggg gatgcaactc
     2101 gttaaaggga ttgtggcaaa aagtattatg attgccaaac tacctctttt gccggcagaa
     2161 ctgggcagga tggcaggcga gaaacttgcc gcttacctca aaggggaaag tactccgtct
     2221 caggaagatg agcagggatt ctggagtcgg gtctgggaat ccattagcaa cccggtggtg
     2281 gacggccccg atccggcttt gcatgtcgag acacggctgc tggacacgat tgactgtcat
     2341 aaacatcgcc cgcaaccgat acagtatctg gcggaaggtt cagaaaccgt acgtattaac
     2401 agccagcccg cctgccgtaa cggtgatcgc agtacctgcg aggcggtgat tgcccatacc
     2461 caggccagtg cgaaagtccg tattggcggg cgcagcctgg tggtgaagga aatacacacc
     2521 ggcaaaaata tgctcgctta ctatgcaggg cttattgccg gaggatttct ggtcagctca
     2581 gtgcaggctc tgttttgcag gacaacggcc agggagtttg cggggaagtt gccctgcctg
     2641 atgctgggtt cggtcctggg caatacgctg gggatagtgg ccggaggaac actttccact
     2701 tcattatcgt ccctgtcttc agggctgcaa gcgtcatcac agaccgccag gccggtacac
     2761 atcgcctccg gtgtaaaaat tctgggcgga gaggatgagc tggattttgc ctgtgacggg
     2821 ttaatcccgc tggcatggca gcggatttac agcagcctta atgacaatga aggcattctt
     2881 ggccggggct ggtgcctgcc gtttgaagtg ttcctgcatc aggagcctgc tgtaccggga
     2941 gcgcaggaca gcgcgcagat attttttcat gacatgtccg gcagggagct ggggctggga
     3001 gatgttccgg tcggcggagc catgttctat gtggacgaag gtttccggct ttatcatcca
     3061 ctggctggta tgttcgtgct ggaaagcgca caggggcagt accagatttt tgaggcagat
     3121 cccctgcacg atggtcggtt ccgcctgatt caggaatgcg atcgccatct gaacggtatc
     3181 cgttattact acaatgaccg gggactgccg gtgcggatcg aggatgatct tcaggccatc
     3241 tgcctgcatc tggactatca cccgcagcat tcccgtctga cggccgtata ccagcagtac
     3301 cggcaggagg ataccctgcc gggcgtactg cgtattcgtt atgaatataa tgaacaggtg
     3361 cagctcaccg ccgtcgtgga tgctgacggg gtcgtgatgc gccgttttgc ctgggatgaa
     3421 gagcacgggc gcctggccat gcaccagacg gcggatggcc tgcaggcgca ttaccgctgg
     3481 cagcccactt ccggtcttcc cggccatttt catgttgccc ggcactggct acaggaaggc
     3541 gagcggcgca gcggggaaat cctgtttgat tacgatccgg cggcgcgccg ggccacgctg
     3601 cattatggtg atggaaggcc ggacagcctg catgaatggg atgagcagta caacattacc
     3661 cgctggcagc ctgccccgga tatctgctgg ctgtatgaat ggggggataa ccgggaactc
     3721 ctgcgggtgg ttgatcccgc acagtgtgag tacggcttca agtacgatga gcgcggcaac
     3781 ctgatccagg tggttgatcc tgtcgggcag gaataccgat ttgactggga ccgggatttt
     3841 gcctttccgc tgaaagagga atatcccgaa gggctggtct tacgccatgt ttacaatgtc
     3901 tctggggatc gcatcaaaac cattgaccag gcaggcctca tcacccgcta taaccatgat
     3961 gcttccgggc tggttgagag cgtacaggat gcacggggca acctgtaccg ctacgcctgg
     4021 aataatcgtg ggcagttaac ccggcagacg gactgttccg ggtataccac ccacctgtat
     4081 tatgatgagt atggacggct ggctgccgtg acggatgcac agggtaactg cacccgccag
     4141 acgttttcag ctgcgggccg cctgcaaaag caaatccgcc ctgatggcgg ggaaaccacc
     4201 tgccattaca atgcccgtgg acagcttgcc ggtgtgaccg atccggcacg tcagagcagc
     4261 cggtttgact ataacctgcg cgggcaggtt atccgccaca ctgatcctca gcagcgccag
     4321 gtgcgcttcg aatatgatgc cggcgggcag cttattgcgt tgtataacca gaaaaatgaa
     4381 gcgtaccgtt tcagccggga cagcctgtca cggctggaag agtcctgcga tctgacgggg
     4441 tgccgcgagg tctaccggta tgacagccgc agtcatgtca gcgaaataac ccgttatccc
     4501 gcccccgccg tatccggtca ggagacgttt ttaccgcagg tcacccggct ggcgtatgat
     4561 gccgccggac gggtgcagta ccgggagaac ggagatggcg ctgtctgtta ccggtatgga
     4621 aaaaacacgc tgtcacttga gcggtttgcg ccggatgact ggcagaagaa acagcgcggg
     4681 tatgaaaata tcgctgcgca ggacacgctg catctggtca cggatgcgta tggcagcctg
     4741 ctggaagaac ataacccggc gggcatcttt acccaccgct atgacgaact gggcaaccgt
     4801 acagaaacgc agatgccgga tggtcgccgc ctgaagcggc tgtactacgg cagcggtcac
     4861 ctgcaacagc tcaacctgga agacaacgac gggcagcaga cggtactggc tgagtttgaa
     4921 cgtaacagcc tgcaccagga aatcagccgg acccagggca gcctgacact gcgcacggaa
     4981 tatgatgccg ccgggcggat tactgcccgt ctggcgcaga tggcgcagca gcgacatgga
     5041 acaccgttgc tggagcggcg ttacagctgg gatcagcggg gcttcctgat gagccgttac
     5101 caccggacac aggatgcgca ggcactgctt tccccggagg aacgcagtgg cagcacctgg
     5161 taccagtatg actgcgccgg gcaaatactg agcgcacagc agcacttcac ggtgcggcag
     5221 tactgcgatc tggccggaaa cctgatggag gagcgcagtc cggcgatcaa gaataaccag
     5281 ttaatgcagt accggggctg gcactaccag tatgatgcct tcgggcggat gagcaggcgc
     5341 acggatgaac aggcgcaacg gcaggattac cgctataaca gcgataaccg gatggtggaa
     5401 gtgcggtttg cggacaggac gcactcccgg tggcagcggg tggaatacga ctacgatctg
     5461 ctcgggcgac ggacggggaa gcggctgtac cggtggcatg atgagggcga agcggaaccg
     5521 gctccggaaa gggtgcgttt tatctgggac gggatgcggc tgcggggtga agacagcccg
     5581 ggtaaggcca cagaagtcct gtatgtctat aacggcgaca gttatgagcc gctggccagg
     5641 gtggatcgtt ttacccggca ggtgatagaa ccgcaggaca cgcgcccgcc gaaaaccacg
     5701 gtatattact accacacggc gctgaacggc ctgccggaag cgatgaccag tgaatcaggg
     5761 caactggtct atgaagcgcg ttatgaactg tgggggaaag aaacggaggc tgcaagcgca
     5821 catacggtcc tgacagtggg gcaaaacctg cggttcgcgg ggcagtattt tgacgcggaa
     5881 acggggcttc actataatac gttcagattt tatgcgccgg aatgtgggcg gtttacccag
     5941 cctgacccga tagggctaag aggcgggctg aacctctata gttatgtacc gaatccgctg
     6001 tcatggatcg atccacttgg tcttaagtgt tggtcaacag aacaaaaaaa ttattggaaa
     6061 gatgttggtg caaaaga
//
LOCUS       DAZGCQ010000209         6040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000209 DAZGCQ010000000
VERSION     DAZGCQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.209"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004994"
     CDS             complement(<1..158)
                     /gene="tnpB"
                     /locus_tag="ACHK07_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8105200.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGK"
     gene            complement(163..831)
                     /gene="tnpA"
                     /locus_tag="ACHK07_004995"
                     /pseudo
     CDS             complement(163..831)
                     /gene="tnpA"
                     /locus_tag="ACHK07_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            1169..1310
                     /locus_tag="ACHK07_004996"
                     /pseudo
     CDS             1169..1310
                     /locus_tag="ACHK07_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1404..2630)
                     /locus_tag="ACHK07_004997"
     CDS             complement(1404..2630)
                     /locus_tag="ACHK07_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8105201.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(2703..3725)
                     /gene="iolG2"
                     /locus_tag="ACHK07_004998"
     CDS             complement(2703..3725)
                     /gene="iolG2"
                     /locus_tag="ACHK07_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFO8105202.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFTLRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(3898..5838)
                     /gene="iolD"
                     /locus_tag="ACHK07_004999"
     CDS             complement(3898..5838)
                     /gene="iolD"
                     /locus_tag="ACHK07_004999"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFO8105203.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         1744 a         1559 c         1231 g         1506 t
ORIGIN      
        1 attttgccac tgcggccccg gaagacgaag atatgcccgg agaacgggtc atctttcagc
       61 gtgttctgca ccttcgaagc caggccgttg aagccacaac gcatatctgt gatgccagcg
      121 atgatccaga ttttggtacc ggtcggcagc gttatcatca gatccccctt tcatttcgcg
      181 aatgagcacc ctaagaagtt ccggagtgag gggggaaaac agttttacca cacctgattt
      241 aagatgcagc tcacactgtg gcgagcctac ggtagtgtca ttctggcttt catcagactt
      301 gtttcgccag aaaggatttg tcaccggttt gacggggtca agagtctcag tgattgccac
      361 cggaacggcc tggcattcct ggagatcctc atcattcagt aagccctctt catacagtct
      421 tttccagtta aacaacagat tgtcattaat attgtgctct ctggcgatcc gtgcaacgac
      481 tgcaccaggc tgtaatgcct gttttaccag cctgatttta aattcacgac tgtaggaggt
      541 tcgtcgttct tttcgccatg tgctttcgct gatttgagga tcatcaggta attccttctt
      601 tctattggca taaagtatgg catcgagttg ggcgactgaa agtgattcag gtaatggcca
      661 agtaataccg gaggcattaa atcgttgaaa gagattatga atggtggaat gactgagacc
      721 taactgcagg gcgatagccc ggacagataa ttgctcgtcg aatcttagac gtaatgcgtc
      781 tggcaaataa gagcgaaagc cggtgatatc ttttgctgtc tgagaattca tcgttcgtgt
      841 ccatcaatat agatggacgc gattgttgcc agacaggaca accatcacaa gacgtcacag
      901 gcagagcgct tacggaatgg ccttccaacc gtgttgacga actgctgcca tggaacgtag
      961 ttctcaccga taaataagcg tcaatacggt gctctgttga cgcttacctt ttactgcgaa
     1021 aatggacatt agtatcggaa acaggaaagg gaggcgaaag acggtttaaa tgatgcgctt
     1081 acccgcaggg ttgaaataac cggggaaaca tcggtgactg attaacaatg aagtgccccc
     1141 ggagtaattc cgggggccgt aaccggaatt tattttcttt ggctgtgctt atgaaagtga
     1201 atgaggagcc ctgctgtacg gggtgagtct catgcttcca gaagcacatc tgttatattc
     1261 tgagcgttct gccagaatgg ccttcctgcc gtgttgatga actcctgtag aacgaagtac
     1321 ttcgccaata aataaccgtc aatacggcat tcgattaatg cacttacagc taaatggtgt
     1381 ttttgaacaa tatttgacgt tatttatttc tcaccaggaa ctttaagaaa ctgaaccagg
     1441 atgccactaa ataaatagag tcctgccata gtatacataa ccacgcctgt accaaaacct
     1501 gtcagaatgg ataccaacgc aggtccgaca aaatttccca ggccagaacc gaaattcacc
     1561 gctgagtttg ctgccccctt cttatccggt gccatcatcg gtaccagcgg cgtcatcggc
     1621 acaaatcccg ccagccccgc tccccacagg cacattgcca gcataaaagc aacgtaatta
     1681 tgcccccaca ctatcggcac ccagtagaca atcagcgtgg caaacccggt cagaagactg
     1741 ccaaaaatct ggatagtacg gcgaaaacct attttatccc ccatccagcc gaaaataatg
     1801 ttgaaaatct gattagaaat aaaggttata ccccagatac tcgcccactc ctcaagggta
     1861 aactgtttat ccagcaaaaa cagcggcaac accactacaa aaccataggt gccgagtcca
     1921 ttgattgttt taacaatcac tgccagacca attcggggtc gttgatacat aatggaaata
     1981 cctgccgaca actctttaaa gactgacgta gttcttggct tcagaatgaa cgcattgcgc
     2041 tcaaagacca gcatgaaaat ccccccaagc agtgcaaccc caatcccggc ccacataaca
     2101 ttaatatgcc caaggacagg aattaacgaa gcagctacta ccggaccaat gatggtaaaa
     2161 cctaagttat atactaccca aaacagcgac gttccaattc cctgttgatg attatcgagt
     2221 tgaatggtca gtcggacaag aaaggaatag cagaccaacg gataacctac tccccgcagc
     2281 atatagctaa tgagaattgt actaaaatca tggctgggaa tcgcaaaatt aagaaacgcg
     2341 atagaagcga tcaggtaaac ggagaaacca gcaagcataa cctttctggg gccaaacata
     2401 tccacacaga caccggataa ccaggaagtt acggcaacca caattccgta ggcggcaaat
     2461 atctgtccga tctgagactg atcaaatccc tgagtattca gccaggcaga tagccaacta
     2521 ttttcaatac agccgcccaa cataaataaa aatgttccca ggtagctcca gataaactga
     2581 cctggcttta tataaaatgt ctgaggcaac attgcctgca tatctttcat tttataaccc
     2641 tcagtacgga taatctctcg ctcgtaatag tgcgagagac ccttcgctat ttacaaatgc
     2701 agctaaagtt gaatatcagt ttttatcttc actggcagtc cactttttaa tgattgtttt
     2761 gcagcaatgg caatacgctg cgccaataat gcatcttcac cagtacagac aatgggtgta
     2821 tgattcagaa cagaatcaat aaagtgtaat atttccgttc tataagcttc acgatagcgt
     2881 agcgtaaaat caggcaaagg atttttcatg atacagttct gactgttata taccttcacc
     2941 gccgattcac tgacattgtc ggcatacgcc atccctgctg aaccaaagac ctcaacccgt
     3001 tggtcgtagc cataaaccgc ccgacgacta ttatcaatta gcgcataacc accgtttctg
     3061 aaggtcagca tcaccacgag agtatctacg tcatcaatat ttttcagagt aggatcaatc
     3121 aatgtattgc ctttaacata aacctcgcta acttcatcct gcataataaa gcgagccata
     3181 tcaaagtcat gcatagtaaa atcaaatatg agtccaccta tcctcttgat caaatcgtgc
     3241 ggcaacaact ctggatcacg tgaggtgatc tttatcatct gaggctgacc aatcttgcct
     3301 ctgcgcacca gttcatacac ctcccgaaac tggggatcaa aacgacgatt aaaagcaatc
     3361 tgcagtttga ctcctttctc tttaaccgcc cttagcaccg caagggaagc tttctcatct
     3421 tctctcatgc tgagaggttt ttcgcagaaa atatgtttac ccgcctggac tgctgccgta
     3481 acgatctctt cgtgcatatc tgtcgaggtg aaaataaaca ccgcctcaat atcatcacgc
     3541 tgtaacattt cctgataatc tttatatcct gaggttatat ttaccgaact cagccagtcg
     3601 ctcatttcct caataaaact gtcagcagca cagacaatat ctaattgagg aagtaaatac
     3661 atattctcaa catgcatttt ccctacccgg ccaagaccaa taatcccaca tcgaagcttt
     3721 tgcatttata cctcgtcata tcatgatata gtaaatataa aacgacaaaa gaaaatcccc
     3781 tttatccaac acggtacgaa agactttatt ttgtagtttc aacgaacaaa tacttttacc
     3841 gtaaaaaacg tcaggttgca gaaaggcgcc ctcctgcaac cttaaaaact gaattaatta
     3901 gtactgtcgc gcttgtccaa tatgctcatc aagcatacga gctactgcct ggatgcgttc
     3961 gctacgcgac acgcgagcgc caccaacacg ccaccagctc agatatttat gcgccattgt
     4021 tttaggcagt actttaatgt ctatcaacgt actcaccgtc tgtttacgtg catcctccag
     4081 agcttcaagc agttgtccaa tgctggtgac gcgatagctt ttacagccat atccttcggc
     4141 aatccgggca aaatccacag gaatgagtcc cccctcctga cgaccgttat cctgtttatg
     4201 gaaacgaaac tcggtaaaga aactgtccat accgtgttcc atttgaagat tatttataca
     4261 accattagtc atgttatcca gcaaaataac attaattttc ttccccatct ggaccgaagt
     4321 taccagctca gaatgcagca tcataaatga gccgtcgcca acgaagctat atacctcccg
     4381 ctccggttgc gccaacttcg cccccaatgc ggcattgacc tcatagccca tacaggaata
     4441 gccatattct acgtggtaat cattatcgcc acggctttgc cagatgcgct gcagatcgcc
     4501 tggcagactt cccgccgcag caaccaccag cgcgttttct ggcagcattc gatttagtac
     4561 tccaagaacg cgcgtctgtg tcagccatga atgagtaatt tcattgaact cttcaaacac
     4621 tttttgagta tccatatgat ccacaatttc cggtttgaaa tccttaccgc tgtattccgc
     4681 tgcatagacg cgatccactt ccgccagata ttgagaacga acacgatgga tcgttttccc
     4741 ccattgtgat atgtaacctt ccggagccag tcgttcgcaa agctgcgtca gggcaacacg
     4801 ggcatcagcc tgcaattgca ctccatccag tttgaagacg tcaaaacggc tgacgtttat
     4861 attcagatag cgcacatcag gattctggaa aatccattta gaagaggttg tgaaatcggt
     4921 ataacgcgta cccacaccaa taatcagatc ggcctcctga gccagcaaat tcgccgccag
     4981 cgatccggtt tcaccaatcc caccaacatt cagcggatgc gcagaactga gcgcaccttt
     5041 accagcctgc gtttcagcaa tcggcagatg atattgttcg gcaaatcgca gcagttcctg
     5101 ttcagactgc gaatacttca cacccccacc gcaaataata attggtctct ggtggcgaag
     5161 aataagagca actgcatcat tcagcattag ctctgttggc acgtggcgct caagacgata
     5221 gacccgacgt gcaaaaaaat agtctggata atcccaggct tctccctgaa catcctgcgg
     5281 caaacaaagc gtcacagctc ccgtatcagc cggatcggta agcacccgca tcgcgctaat
     5341 gcaggcgctc attagctgtt ccggacggat aatgcgatcc caatagcgac tcactgcacg
     5401 aaaagcatca ttggtgctca gcgtcaggtc atgctcctgt tcaatctgct gcaacacagg
     5461 atcgggttga cgcgatgcat acacatctcc tggtagcaga agcaacggga tacggttcgc
     5521 cgtcgcagtc gccgccgccg tgatcatatt agcggcacca ggccctacgg aggaggtgca
     5581 ggcataaatc tgctgacgac gcatctgacg cgcaaatcct gttgctatat gtgccatacc
     5641 ctgttcattg cgtccttgat gaacgctaag ttgaccacac tcttcttcca gagcctgccc
     5701 taatcccacg acattcccat ggccaaaaat tgcgaaaata ccttttacaa acttgatttc
     5761 gcgaccgtcc acttcaatgt actgattatc aagaaagcgc accaaagcct gcgccatagt
     5821 caacctgatt gttttcatgc actccaccta tcttatcaac cgtttgactg tcgttaccta
     5881 cgtacgcact gctacagccc cgggcataaa acactcacca caagaaaaaa attccgcaaa
     5941 cgcaattata tgaaacatat ttttcatttg tgggaagaag atcaaaaaac gattaaaaat
     6001 ttcgagaata gctaaaatac gaacaaagaa gaatgaacat
//
LOCUS       DAZGCQ010000210         6002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000210 DAZGCQ010000000
VERSION     DAZGCQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.210"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(368..1087)
                     /locus_tag="ACHK07_005000"
     CDS             complement(368..1087)
                     /locus_tag="ACHK07_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105204.1"
                     /translation="MSKLKNEPLVRVSEEDGIEIRKIQYPDNTIERIYKQKGVILPRI
                     PLKGRFVEQYVALQLLDKDLRNVIGWENIIKNICNNINKEQHFIYPDLEKNLILKSLF
                     ISKVVTYGKCFTEAKGRRFTLQRKHVPEKYRDLHDSIMNIRHNFAAHKGEFEYDQCGL
                     VLILPGTKKKRYYHIFSELNQINYGNNEEDDERFFMLYDSLREVIKEKQDKLIDKIYA
                     EKIHTQTMEYWLSRAGKETEI"
     gene            complement(1373..2596)
                     /locus_tag="ACHK07_005001"
     CDS             complement(1373..2596)
                     /locus_tag="ACHK07_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8105205.1"
                     /translation="MALSDVWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKVTEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRCLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYLAWW
                     KRVESIVNCTISDSN"
     gene            complement(2982..3659)
                     /gene="yjjG"
                     /locus_tag="ACHK07_005002"
     CDS             complement(2982..3659)
                     /gene="yjjG"
                     /locus_tag="ACHK07_005002"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO8105206.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(3674..4120)
                     /gene="rimI"
                     /locus_tag="ACHK07_005003"
     CDS             complement(3674..4120)
                     /gene="rimI"
                     /locus_tag="ACHK07_005003"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8105207.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(4089..4502)
                     /gene="holD"
                     /locus_tag="ACHK07_005004"
     CDS             complement(4089..4502)
                     /gene="holD"
                     /locus_tag="ACHK07_005004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO8105208.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4605..5636
                     /gene="rsmC"
                     /locus_tag="ACHK07_005005"
     CDS             4605..5636
                     /gene="rsmC"
                     /locus_tag="ACHK07_005005"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO8105209.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGIEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            5904..5990
                     /locus_tag="ACHK07_005006"
     tRNA            5904..5990
                     /locus_tag="ACHK07_005006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5938..5940,aa:Leu,seq:cag)
BASE COUNT         1566 a         1456 c         1336 g         1644 t
ORIGIN      
        1 tgaccttcca gatcctgata ttaaatcctg cccaaataaa tgggcatcac acaagattta
       61 cagatttgcg ggagtgattg agtaatatgt attagcttag atgtgagctg acacatctat
      121 gcttagatgc caaacctaat ctgagcgtcc actctttacc aagagcggac gttaccaatg
      181 accatatgac atgaatcgat aagatcaaaa cattatcgcc aagaaatgtc ataccattga
      241 tacaaataaa attgattacc cccttactct tgttgaaaag ttagtcgtag agtacctatc
      301 gcaatgtaat cagctttaat catctgaaaa ttactcctta aaaattagct tttatatact
      361 gaacaaatta aatctctgtt tcttttcctg ctcggctcaa ccaatactcc attgtttgag
      421 tatgaatctt ttcagcgtat attttatcaa ttaatttatc ttgtttttct ttaatgactt
      481 cacgcagtga atcatataac ataaaaaaac gttcatcatc ttcctcatta ttaccgtaat
      541 ttatttgatt gagctctgaa aaaatatgat agtacctttt tttcttggtc cccggcaata
      601 ttaaaaccaa gccacattga tcatattcga attcaccttt atgggcagca aagttatgtc
      661 taatattcat aatagaatca tgtaaatctc tatatttttc tggcacatgc tttctctgca
      721 atgtgaatcg acgccctttc gcctctgtaa aacacttacc gtaagtaaca acttttgata
      781 taaacaaaga ctttaatatt agattctttt ctaaatccgg ataaataaag tgttgttctt
      841 tattaatgtt attacaaatg ttttttatta tattctccca accaataaca ttccgaagat
      901 ctttatcaag caactgtaag gccacatatt gttctacaaa gcggcctttt aaaggtattc
      961 gtggtaatat tacacctttc tgcttataaa ttctttctat cgtattatct ggatattgga
     1021 tcttccttat ttctattccg tcttcttctg atacccttac taatggttca ttttttaact
     1081 ttgacatgtt aaccctctta aataaaaaac taactttatc cagatagtgt ccgttaagaa
     1141 gttttcgtta caactaagtt tctaggtaaa tcatgaatcg acctatagta tcaaaaacat
     1201 ttggtggtaa acatctgcat ttatcaaatg gcaggagcga tttaggggca tagggatagt
     1261 aatgatatcg atacccgccc cctcttatgt caacacaatg gcgtaaactt aacgctcaca
     1321 tctcgctcaa agtgtgctag gagatttggt tatgttcagg tgatacaaac cgtcagtttg
     1381 aatcagagat agtacaatta acaatcgatt caaccctctt ccaccatgcc aggtaagctt
     1441 tacgctgttc ttctagataa tcactcttat cataaacttg ccatacccct ggcagcttat
     1501 gaccaagcat aatttctgcg atatgaggcg cagtaagatc agaaaaattt gttcgagctg
     1561 tacgtctcaa gtcatgaaga gaccaatgag ggaattgata tcctaaacac cgccatgcgt
     1621 actgcattaa attgtaaggc agcgactgca atgatgtccg accaacgggc tccctgcttc
     1681 cttccttagt aaaaagcata tcggaaccat tgttcataga gatagcgtac tttataagct
     1741 cttcaaccgg ttcaataatg ggccgcttta gcggttcgcc tgttatctcc ccagtcttat
     1801 gtcgttctgg tggtacagtc catactttat taatgaaatc aaaatcgtcc acccgggcgg
     1861 taattagctc tgaactacgg caaccaaaat gcagcaatag tttaatgaag gcccggtatt
     1921 taggaaccat tcgagaacca tcgatcgcag cataaaggat tttaatttca tcatgtgtca
     1981 gaaaccgttt cttctgacct ttacggatat ccatatcttt acccgtgata tccgacagcg
     2041 ggcgagtttc aatgagcttt ctcttatacg cccagacatg ggcctgcttt gcgttaatta
     2101 gcaatcggtc tgctattgct ggagtcttag tgctaagagg ctccaagact tctaaccaat
     2161 catgcaatgt agctgcatcg tgagggatat tcccgatttt agagaacagg tgcagctcaa
     2221 acgagcgaag tatctgttca gaaccttttt tattttttac acaatatgct tcataccaag
     2281 cacggatcac agactctacc gtcatggctt cagtaacttt tcgtttttca gcctgcttga
     2341 ccaatcgtgg attacggttt gactcgagtt caccacgaag acggataact tcttctctgg
     2401 cctcttttaa tccagttgcc gggtaagttc cgatatcaag acgctcacct ttccctgccc
     2461 attgataacg atattggaac actacgcgac ctttcggtga tactctgaca gacagaccat
     2521 cacgatcgga tttaaccaaa accttatcac gttcctttcc aacgactgaa cgcaaccaca
     2581 catcagacag cgccattact caccttgtac aaatcgcaaa acacctctgc tgttttgtac
     2641 attatgtaca agcatatgta cagatttttg atgaaagcaa gctgatcgat ttaaagttac
     2701 atgaaagagt tttcaattaa agaagctaca taaccaaatg tatttaaatg agttatataa
     2761 ctcaacctaa attaactgaa aggattttga aagagtaaaa gagctttaat ttgggcttaa
     2821 ttattggggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttg caaataagga
     2881 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
     2941 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
     3001 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
     3061 cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
     3121 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
     3181 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
     3241 agcaaatcga agtaatcacg caggcccgta cgttccagac gcacttgctg caaggcgcta
     3301 aagccgttgg tgatgatgcc gattttggcg ttgccacgaa tggcgttaag cagagaaacc
     3361 gcgcccggca gcggcgtaca gatttccgcc atcgcattaa taaaggcttc gttgagttta
     3421 cccggctcga cgttcagccg ttcggcccag ctctcaaacc gcccgtgctg aagctgtaat
     3481 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
     3541 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
     3601 aatgagtcaa aggtaaacaa cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
     3661 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
     3721 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
     3781 gggcaatggc ggcagcgttt gaggcacgga cttccagcca gagcgtcgcc acgccgcgtt
     3841 tttccagttc gtcgatcaga tgttccagca gcgcccttcc caatccctga cgctgatagt
     3901 caggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
     3961 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
     4021 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
     4081 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
     4141 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
     4201 gcgccttcca gtgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
     4261 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
     4321 aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc gtttgccacc
     4381 atcaccagac ggacgtgtgc cgggatcgca atggcaatct ccccctgcaa cgcgccaggg
     4441 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
     4501 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
     4561 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
     4621 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
     4681 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
     4741 cccagcaatt ccaccactgg caggtattaa gccgccagat gggggataac gcccgtttca
     4801 gtctggtcgc cacggcggat gacgtcgcag attgcgatac gctgatttac tactggccga
     4861 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccagtgggga
     4921 cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgccgag cagatgctgg
     4981 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggactc tattttggtc
     5041 gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac agcgtcgatg
     5101 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
     5161 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
     5221 gcgcgggtgt gctttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
     5281 gcgatgtctc tgcgccagca gttgaagcca gccgcgcgac gcttgccgcc aacggtattg
     5341 aaggtgaagt ctttgccagc aacgtctttt ccgaggtaaa aggtcgtttt gatatgatca
     5401 tctccaatcc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
     5461 ttcgcggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
     5521 tcctgcctta tccggacgtg ctggatgaga catttggctt ccatgaagtt atcgcgcaaa
     5581 cagggcgctt caaggtgtat cgcgccatta tgacccgcca ggcgaagaaa ggttaagtat
     5641 cccgccgaat actgtgtcgg atgcggcgtg aacgccttat ccgacctaca aaacctgcac
     5701 gttagccctt cgtaggccag ataagacgcg ccagcgtcgc atctggcatc tgcccccgca
     5761 tgcctaccaa acctgcacgc tagtttcctg atggacattt ttccagcaat tacacctccg
     5821 gcgataatta actattgacg aaaagctgaa aaccactaga atgcgcctcc gtggtagcaa
     5881 ttctttttaa gaattgatgg catgcgaagg tggcggaatt ggtagacgcg ctagcttcag
     5941 gtgttagtgt ccttacggac gtgggggttc aagtcccccc cctcgcacca aaaaccacgt
     6001 tg
//
LOCUS       DAZGCQ010000211         5995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000211 DAZGCQ010000000
VERSION     DAZGCQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.211"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(172..1137)
                     /gene="alx"
                     /locus_tag="ACHK07_005007"
     CDS             complement(172..1137)
                     /gene="alx"
                     /locus_tag="ACHK07_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO8105210.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1421..2407)
                     /locus_tag="ACHK07_005008"
     CDS             complement(1421..2407)
                     /locus_tag="ACHK07_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8105211.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(2486..3178)
                     /locus_tag="ACHK07_005009"
     CDS             complement(2486..3178)
                     /locus_tag="ACHK07_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO8105212.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATLFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(3255..3758)
                     /locus_tag="ACHK07_005010"
     CDS             complement(3255..3758)
                     /locus_tag="ACHK07_005010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO8105213.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3843..4979
                     /gene="rlmG"
                     /locus_tag="ACHK07_005011"
     CDS             3843..4979
                     /gene="rlmG"
                     /locus_tag="ACHK07_005011"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO8105214.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAREMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            5263..5577
                     /gene="higB"
                     /locus_tag="ACHK07_005012"
     CDS             5263..5577
                     /gene="higB"
                     /locus_tag="ACHK07_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO8105215.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            5574..5990
                     /gene="higA"
                     /locus_tag="ACHK07_005013"
     CDS             5574..5990
                     /gene="higA"
                     /locus_tag="ACHK07_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO8105216.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         1568 a         1563 c         1473 g         1391 t
ORIGIN      
        1 cagcttatcc cttgcggcta ccgggtcggg cgcaagggaa cgcttcggcg attttgacgc
       61 caccaacacc cgtgcggtct ttttgtaaag agctataagc cgattacata attaatagct
      121 cacctttgtc tggaatgagt tatctctagt aatggcttaa tatacagcga atcattctcc
      181 ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca tcaccaggat
      241 gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga caatcagcat
      301 cttgataccg ataaatacca gaatcaccgc caggccatat ttgagcatcg agaaacgctc
      361 cgcgacgccc gccagcagga aatacatcgc acgcaggccg aggattgcaa agaggtttga
      421 ggtcagcaca ataaacgggt cagtggtcac ggcgaagata gccggaatgc tatccacggc
      481 gaaaatcacg tcgctcaatt ccaccagaat caataccagc atcagcggtg tggcgtacaa
      541 caagccattc ttgcgcacaa agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg
      601 accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt catgggcgag
      661 cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga tccagtcgaa
      721 ctgcgaaatc agccagctgc ctgtgaagat catgatggta cgcagaacaa tcgccccgag
      781 cacgccatac accagcacgc gacgttgtaa tgccgccgga acggagaaat agctgaacaa
      841 catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat aacccgtgag
      901 aaaggccagt gcctgtggat cggcaacggc gcgaccttcg gtttgcacca gataccacca
      961 gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg ccgcagcctg
     1021 tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga tagccagcat
     1081 aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag tattcataga
     1141 agttccttac acataaaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg
     1201 catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat
     1261 tcctttcggg ttgcccggtg accggatgtg atttttcaca catcgtaatg acgatcgacc
     1321 ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc acccagtcaa
     1381 tggtctgtta catgcctttt acactgcttt acggtaacaa ttattgtttt acgctatctg
     1441 ccggaaaaat cacccctgtc tggcggcgga tctctgtcag caatttggcg gtgatgcgac
     1501 tgaccgccag tcccggatga tccaccagat gctcatccac cagggtagcg aacagctctg
     1561 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
     1621 gcggcacaaa gcatactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
     1681 cgccctgaat ttcgcttgcc aggacagaat cactgacttt tgagtgctgc aacgtaacgc
     1741 tgaaatcacc gtaatccatt accaccacgc cgtgggcatc tacgccgctt gccagcaaac
     1801 tggcggttgc ctgcacgctt ttcggctcac caaataacgc caccgccgac gccaggcagt
     1861 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
     1921 caccgtccag gtaacgttga taccgcgagg aatactggca atagttgaaa aaaactttcc
     1981 gcagtttgcc gactttcggc agcgcctggc gcaacaaatg aaagttcggc aggctggcgg
     2041 ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg gcggcatcca
     2101 cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta tggctaagga
     2161 aaagttgtgt ctgggaaaag tgcagggaat tcgggctggc aatatacacc gcgtcaatgg
     2221 catcgctttc tgccatcgct tccagcgacg taaacagatg ctcgacagaa aaatcattgg
     2281 cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg tatttaccgc
     2341 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
     2401 gtatcataac gcgtggatac tccataaagg gattctgccg tcaatgtagc acgccttttt
     2461 catcttcgac gtgcgcggat acgcattatt tctgcgctgg acaaccatgc tcactaaacg
     2521 gaccaataat gaccgacggc ccgagatatt ttggctgacg atggaagatt ttcgcgtcaa
     2581 tcaccgcgct gattctggcg agtttctccc gtaactgagt ataaaaaccg tgcttcatat
     2641 tcaggtctgg tgcgttaaac ccgatagcat cgatgccgta ttgttttgcc agccagatgg
     2701 cgcgttgatt atgaaattcc tgcgagataa tggtgatatg gttttcccca aacacctttt
     2761 tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcagaag atcactttcg
     2821 ccggtacgcc tttagcgatc aaagcctgct gcatacctga tgcttcatcg taatttttac
     2881 gtccgttatc gccactgact aacagccatt tcacttttcc ggcgtggtac aactctgctg
     2941 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
     3001 cgttacgcgc cggaacgacg ttgacgtcgc tccaggtcag ctgtttgctg gcgttgacca
     3061 ttacgcgatc ggcaataaaa agggtcgctc ctgccacaag taacagtaag caggcaatct
     3121 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg
     3181 tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac
     3241 gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgcgttag ccacagccgg
     3301 gtgtcgaact caagctggtg gtactgcggc tccatatggc agcacaattg atagaacgct
     3361 ttgttatgct ctttctcctt caggtgcgcc aactcgtgca ccacgatcat gcgcaaaaac
     3421 ggttccggcg cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc tttcagcttg
     3481 ccgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag cacatggatc
     3541 ttattgtcat acatcacttt attgatcggc ggggcattac gcagaaactg atttttcaga
     3601 tcctgagtat attgccagag ggctttatcg gtggcgtagt cgtgtgttcc cggatagcgt
     3661 tttgccagca catcgcccag acgctgttcg ttaatcagtg ttcgcacctg ggaaagtagc
     3721 tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa
     3781 aagggtatac tcacgcaccc tttgcagggg caacgtcgaa attttatcat tcaggagggc
     3841 cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttc ccggcgacgg
     3901 atgacgttaa cccgctacag gcgtgggaag cggcggatga atatttgctg caacagttgg
     3961 acgacacaga aatccgcggc ccggtgttaa tcctgaatga tgcttttggt gcgttaagct
     4021 gtgtgctggc ggaacataag ccgtacagca ttggcgactc atacatcagc gaactggcga
     4081 cgcgcgagaa tttacgcctt aacgggattg atgaatcgag cgtgaagttt ctcgacagca
     4141 ccgccgacta cccgcaacag ccgggcgtgg tgctgatcaa agtaccgaaa acattggcat
     4201 tgctggaaca acaactgcgt gcgctgcgca aagtggtcac gccacaaaca cgtattattg
     4261 ccggtgccaa agcccgtgac atccacactt ccacgctgga actattcgaa aaagtgctcg
     4321 gcccgaccac caccacgctg gcatggaaga aagcgcgcct gattaactgc actttcaatg
     4381 aaccaccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga
     4441 ctatccacaa ccatgcgaat gtcttctccc gcaccgggct ggatatcggc gcgcgcttct
     4501 ttatgcaaca tctgccagag aatctcgaag gggagattgt cgatctcggt tgcggtaatg
     4561 gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg
     4621 aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt
     4681 tggatcgctg cgaatttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta
     4741 atgctgtgct ctgcaacccg ccgtttcatc agcaacatgc gctgaccgat aacgtcgcca
     4801 gggagatgtt ccaccacgcc cgccgctgcc tgaaaatcaa cggcgaactg tatatcgttg
     4861 ccaaccgtca cctggattac ttccataaac tgaagaagat tttcggcaac tgcaccacca
     4921 tcgccacgaa taataaattt gtggtgctga aagcagtgaa gctggggcgt cgtcggtaag
     4981 attgacagct acctgccaga taaggcgtaa acgccttatc tggcatacgt ttagcacatt
     5041 acccccaggt cggataagat gcggcaagcg tcgcatccgg caatgtctgc ccagttctgg
     5101 aatcgcgctc cataaaacaa aaaatccctg tcaccatcct ctacattctc ttgtttagcg
     5161 ttgttcaacg tttattcttc cgtcacacag ataaattcat ccgttgccaa tctgtcaacg
     5221 gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct gataactcaa
     5281 aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt ggtggctcta
     5341 gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc agtattccca
     5401 tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg gggcaatgaa
     5461 ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg tgaagttatg
     5521 acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa aaaatgattg
     5581 cgattgccga catcttgcat gcaggagaaa agctgactgc tgtggcacct tttctggcgg
     5641 gtattcagaa cgaggaacaa tacatccagg cgctggaact ggtagatcat ctgctgctca
     5701 acgatcctga aaacccctta ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat
     5761 cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc ggtatagccg
     5821 tgattcgtac ccttatggat caatatggtt taaccctttc cgatctgccg gaaattggca
     5881 gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg gaacacgcta
     5941 aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa cgcga
//
LOCUS       DAZGCQ010000212         5754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000212 DAZGCQ010000000
VERSION     DAZGCQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.212"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1551
                     /locus_tag="ACHK07_005014"
     CDS             <1..1551
                     /locus_tag="ACHK07_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO8105217.1"
                     /translation="SLLDALTLLLSPESDLYHFERDDFWFPPGDINGREHHLHIILTF
                     RESLPGRHRVRRYRPLEACWTPCADGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPI
                     DVEDINDQARHLVRLMPVLRLRDARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSH
                     PQNLSDGQIRQGLSAMVQLLEHYFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMI
                     DRPGGRSYRVILLGLFATLLQAKGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLN
                     LLPLQRIATTNSGELLSLTPVEHVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRF
                     NRPSSLFARCWLLVEGETETWVINELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARR
                     MGIEWHVLVDGDEAGKKYAATVRSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVF
                     HRVAQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVL
                     WLARGRAD"
     gene            complement(1548..2504)
                     /locus_tag="ACHK07_005015"
     CDS             complement(1548..2504)
                     /locus_tag="ACHK07_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO8105218.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2655..3770
                     /gene="macA"
                     /locus_tag="ACHK07_005016"
     CDS             2655..3770
                     /gene="macA"
                     /locus_tag="ACHK07_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO8105219.1"
                     /translation="MKKRKTVKKRYVIALVIAIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3767..5713
                     /gene="macB"
                     /locus_tag="ACHK07_005017"
     CDS             3767..5713
                     /gene="macB"
                     /locus_tag="ACHK07_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO8105220.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTVPVVNMASGWRQFASGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1322 a         1408 c         1619 g         1405 t
ORIGIN      
        1 agcttgctgg acgccttaac cctgctgcta tcgccagaat cagatctcta ccattttgag
       61 cgcgacgatt tctggttccc gccgggagat atcaacgggc gagaacatca tctgcatatt
      121 attttgacct tccgcgaatc gctgccaggc cgacatcggg ttcgccgtta tcggccgctg
      181 gaagcgtgct ggacgccatg cgccgatggc tatcaccgta ttttttatcg tctggaaggg
      241 gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa agacggacat
      301 ccgattgatg ttgaggatat taacgatcag gcacgccatc tggtgcgttt aatgccggtg
      361 ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta acggcacggt gccaaatgtc
      421 cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt gtcctcacat
      481 ccgcaaaatc tctctgatgg acagattcgt cagggacttt ccgcaatggt gcagctgctt
      541 gagcattatt tctctgagca gggggctgga caggcgcgat atcgtttaat gcggcggcgg
      601 gccagcaatg agcaacgaag ctggcgctat ctggacatca tcaaccggat gattgaccga
      661 cctggagggc gctcgtatcg ggttattttg ctcggcctgt ttgctacttt attgcaggca
      721 aaaggcacat tgcgactgga taaagacgcc cgtccattgt tgctgatcga agatccagaa
      781 acgcgtttac accccattat gctttcagtt gcctggcatc tgttgaatct tttgccttta
      841 caacgtattg ctaccaccaa ctctggcgag ctgctttcgt taactccagt ggaacatgtt
      901 tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc aagtggcttg
      961 agcaccgaag acagccgccg catctctttt cacattcgtt ttaatcgtcc gtcatcgctg
     1021 tttgctcgct gctggttgct ggtggaaggg gaaacggaaa cctgggttat caatgaactg
     1081 gcgcgtcagt gcggacatca ttttgatgcc gaagggatca aggtcattga gtttgcccag
     1141 tccgggctaa agccactggt taaatttgcc cgccgaatgg ggattgaatg gcatgtgctg
     1201 gtcgatggcg atgaagcagg gaagaaatat gccgctacgg tacgcagcct gttgaataat
     1261 gatcgggaag ccgaacgaga acatttaacg gcgttaccgg cgctggatat ggaacatttt
     1321 atgtatcgcc agggattttc cgatgtgttc caccgcgtgg cgcaaatccc ggaaaatgta
     1381 ccgatgaatc tgcgcaaaat tatctcgaaa gcgatccatc gctcttccaa acccgatctt
     1441 gccattgaag tggcaatgga ggccggacgt cgtggtgtgg actccgtacc gacgctgctg
     1501 aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta accgcgaaac
     1561 atcgtggcca tttgtggctg aatagcgtcg agcatctcat agcgccgacg gtattcagcc
     1621 cgttttttac tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg aaggcggtaa
     1681 tggcgttcag catcacatac gccgccaacc gattcccaga aagcgttgta atcggcatgg
     1741 atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc attgctgacg
     1801 gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc catcacgagg
     1861 cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg atgtgggatt
     1921 tcccattttg cgccttgcag tccgccaata aacatcgttc ttttcccctg atattcacac
     1981 agggtaaacg tgatctctgc cagaggaata ccttcgctgt tgcggaacag gattgtgctg
     2041 tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg ctcgccgttt
     2101 ttgccttcca gcttcgccag ttgcagcccg ggggtattca aatataagct gaattcttcc
     2161 gccgacatac acccgcggag taacgcataa tggtaacgta acgcctccag caattgctta
     2221 cggctaagat tcgccgcaag gtaagggcga tgcagacgta caggcagtcg cggctggcgc
     2281 gttaacaata cattgagatt aggccagtgg gaaagttcgt tcatccactc aacgcttaaa
     2341 cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg atgccagaat
     2401 ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct gctgagagat
     2461 tcagatggcg taaaggtccg ttcagttagc tgcgacatat tcatgaaatc aatggttata
     2521 catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg attactaacg
     2581 taaattttag ggttttgttg atatttcgtt gaagttaatg acccggattg gcatatggag
     2641 tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt attgcgctgg
     2701 tgatagccat cgccggactg attacgttat ggagaattct taacgcaccc gtgccgactt
     2761 atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg accggaaagc
     2821 tggacgcgct gcgtaaggtt gatgtgggcg cgcaggtcag cggtcagttg aaaacactgt
     2881 cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggtcatt gatcctgaac
     2941 aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc gcgcagcggc
     3001 agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag caacgtctgg
     3061 cacaaacgca ggcggtttca cagcaggatc tcgacaccgc cgcgacggag atggctgtga
     3121 aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggct tctctcgata
     3181 cggctaaaac caatctcgat tacactcgca tcgttgcccc gatggccggg gaagtcacgc
     3241 aaatcaccac tctgcaaggc cagacggtga ttgccgcaca acaagcaccg aacattctga
     3301 cgctggcaga tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg gatgtaatcc
     3361 acctgaagcc ggggcaaaaa gcctggttta cggtacttgg cgatccactg acgcgctacg
     3421 aggggcaaat caaggatgta ctaccgacgc cggaaaaggt taacgacgct attttctatt
     3481 acgcccgttt tgaagtcccc aaccccaatg gtttactgcg gctggatatg actgcgcaag
     3541 tgcatattca gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg gcgttaggcg
     3601 atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa acacgcgagc
     3661 gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa gggctggaag
     3721 cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga cgcctttgct
     3781 cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg aggtgttgaa
     3841 gggcatcacc ctcgatattt atgctggtga gatggtggcg attgttggcg cttcgggttc
     3901 cggtaaatcg accctgatga atattctcgg ctgtctggat aaggccacca gcggcaccta
     3961 tcgcgtcgcc ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc aactgcgccg
     4021 cgagcatttc ggctttattt tccagcgtta ccatttgctt tcgcatttaa ccgccgagca
     4081 gaacgttgaa gtacccgccg tctatgctgg tcttgagcgg aaacagcggt tgcttcgcgc
     4141 ccaggagttg ctgcaacgac tggggctgga agaccgtaca gagtattatc cggcacagct
     4201 ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca ttgatgaacg gcggtcaggt
     4261 aattcttgcc gatgaaccga ccggcgcact ggacagccat tctggcgaag aggtgatggc
     4321 gatcctgcat cagctgcgcg accgcgggca tacagtgatt atcgtcaccc acgatccgca
     4381 ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg tgcgcaatcc
     4441 tcccgccatt gaaaaagtga atgttgctgg cgggacggta cctgttgtca acatggcgtc
     4501 tggctggcgt cagtttgcca gcggttttaa cgaggcgctg acgatggcat ggcgggcgct
     4561 ggcagcgaat aaaatgcgta ctttactgac catgctgggg attattatcg gtattgcgtc
     4621 ggtggtttcc attgtcgtag taggtgacgc cgccaaacaa atggtgctgg cggatattcg
     4681 ttctattggt acgaatacta ttgacgtcta ccccgggaaa gattttggcg atgacgatcc
     4741 gcaatatcag caggcgctga agtacgacga cttaatcgcc atccaaaaac aaccgtgggt
     4801 cgcttcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttacaata atgttgatgt
     4861 tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca tgaccttcag
     4921 tgaaggaaac acctttaacc aggaacagct gaacggtcgt gcgcaggtcg tggttctcga
     4981 cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg aggtgattct
     5041 ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt cgatgtttgg
     5101 tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc gagttatggg
     5161 ccagtcgtgg ctgaactcca ttactgtcag ggtgaaagaa ggatttgaca gcgccgaggc
     5221 ggaacagcaa ctcacgcgtt tactttcact gcgccacggt aagaaggatt tctttacctg
     5281 gaacatggac ggcgtcttga aaaccgttga aaagaccaca cgtactttac aactgtttct
     5341 gacgctggtg gcggtgattt cgctggtggt gggcggtatt ggtgtaatga atattatgct
     5401 ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag gtgcgcgagc
     5461 aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg tcggtggcgc
     5521 gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct tacccggttg
     5581 ggagattggt ttttcaccgt tggcactgct gctggcgttt ctctgctcga cggccaccgg
     5641 gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag tagatgctct
     5701 ggcacgagag taatttttga gataaaaatg ccagccgatc gggctggcat tttg
//
LOCUS       DAZGCQ010000213         5743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000213 DAZGCQ010000000
VERSION     DAZGCQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.213"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            456..1013
                     /locus_tag="ACHK07_005018"
                     /pseudo
     CDS             456..1013
                     /locus_tag="ACHK07_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1153..2003)
                     /locus_tag="ACHK07_005019"
                     /pseudo
     CDS             complement(1153..2003)
                     /locus_tag="ACHK07_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2261..3130
                     /locus_tag="ACHK07_005020"
     CDS             2261..3130
                     /locus_tag="ACHK07_005020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO8105221.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLFHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(3290..3883)
                     /gene="rclC"
                     /locus_tag="ACHK07_005021"
     CDS             complement(3290..3883)
                     /gene="rclC"
                     /locus_tag="ACHK07_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO8105222.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(3895..4131)
                     /gene="rclB"
                     /locus_tag="ACHK07_005022"
     CDS             complement(3895..4131)
                     /gene="rclB"
                     /locus_tag="ACHK07_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO8105223.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(4240..5565)
                     /gene="rclA"
                     /locus_tag="ACHK07_005023"
     CDS             complement(4240..5565)
                     /gene="rclA"
                     /locus_tag="ACHK07_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO8105224.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGVAVNERGGIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLIK"
BASE COUNT         1643 a         1238 c         1261 g         1601 t
ORIGIN      
        1 ataatcccgc gcccaaccac ctgatgagtg gctacaggca ctggatatat taggtggcgg
       61 tgtactttct tacataaagg tatttccttt tctgcggaaa aggaaaccgg gaaatccccg
      121 gtttttctga caagcagacg ccattatttg tgtctgccta tgttcgttaa ttcgttcatc
      181 aggaaattat ctcaatgtca cgttataaaa caggtcataa acaaccacga tttcgttatt
      241 cagttctggc ccgctgcgtg gcgtgggcaa atatctctgt tcaggttctt tttccactcg
      301 ctgtcacctt taccccagta atggcggcac gtgcgcagca tgcggttcag ccacggttga
      361 gcatgggaaa tactacggta actgctgata ataacgtgga gaaaaatgtc gcgtcgtttg
      421 ccgcaaatgc cgggacattt ttaagcagtc agccagatag cgacgtgacg ctgactgcca
      481 gctcggaaaa tttagttctg gatcctaaag ggacggcgaa aactaatgag caaggacagg
      541 ctgttttcac cggctctacc actatcgcag cgacatatac actcacggcg aaagtggaac
      601 aggccaacgg tcaggtatcg acgaaaactg ctgaatctaa attcgtcgcg gatgataaaa
      661 acgcggtgct cgccgcatct ccagaacgtg tagattctct ggtggcggac gggaagacta
      721 ctgcaacaat gacggttacc ctgatggcgg gagtcaatcc cgtaggagga agtatgtggg
      781 tcgacattga ggctccggaa ggagtgacgg agaaggatta tcaattcctg ccgtcgaagg
      841 ctgaccattt ctcaggtggg aaaatcacgc gtacatttag taccagcaag ccaggtgtct
      901 atactttcac attcaacgca ctgacgtatg gcgggtacga aatgacgcct gtgaaggtga
      961 caattaacgc cgttgctgca gagactgaaa atggcgagga ggagatgcca taatttgaat
     1021 agtatgaaag ttacgatcta ataaagaata atggtgttga aatattcaac gccattattc
     1081 actaaatacc ttgcctgata ctgattaaat agtatcaggc aaggataaaa ctcaataaga
     1141 gatagtgacc aactaaatcg gacgaacggg aataaaaatt tcgcaaacaa catcattgcc
     1201 atccttttct acgaaatctt cattgtaata aaagcgctca atatcgctgc cgttcactct
     1261 ggctaacccc ctggcaggca actctatcag atagatgtta cgcacccagt caggataatt
     1321 ttcccagttt ccttcataac ggaaagaggc ataaagtcct cgggtcgttg tccactcttt
     1381 tggcatgtca gcatattcac ataccgtacc agcaaaagtt tcaaccgtga gcaaatcgac
     1441 cgattttgtt gacggttcaa acgtggagac gaaatagata tccgtcttaa aattattcat
     1501 acatctggct aaatgatgac gtcgcagttt gacttcttca tccggtgatt tattcgacac
     1561 ttcaatatca tatttaaatg aattgccaaa ataggtgtct caggaaagtt gactaatcta
     1621 cactccgtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
     1681 taatcacggt ggcggtatac acggggagag cagccgaata actttttaaa ttcgcggctg
     1741 aatgactgct gtgaatcaaa atgcaaagag agtgcaatat caagcataga tttcgcggtg
     1801 agccggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
     1861 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
     1921 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
     1981 tgtagaatct tctgcctgat catcacattc tccgttaatt ctttgtcata tcgcacattt
     2041 tcgttatgcc ctgggcccta agtgtatcaa aaacagcaag ggcgaaggca aaagcactgg
     2101 tagcgtctgc ttcgttgtcc ccgaaaatag agaggtttca gtcctacatt attaatgaat
     2161 tttttgcata agtgatatca aaatccacat actaatttga ggttacgttt taacgtagac
     2221 tcattgttca tgcctaatgt gcctaatgga gggactgaca gtggaatttt ccgtattaag
     2281 taacaatctg aaaatgccga tgatgggatt tggtgttttt caggttaccg ataaaaacgt
     2341 gtgcaaacag tcagtgttga acgctatccg cacgggttat cgactcatcg acactgcagc
     2401 ggtatacggt aatgaagatg ctgtcggtga agccgttcgt gaagccatat ccgaaggttt
     2461 atgtacccga gaagagttat ttataacgtc taaattgtgg gtgcaggata tgttgaatca
     2521 ggatacggca gcagcaggta ttgaagcatc attaaaaaaa tcgggactag aatatttcga
     2581 cctctattta tttcatcagg ctatgcgcga ttatttcagt gcgtggcgtg cactcgaaga
     2641 tgcctatgaa gagggcaaat taaaagcgat tggggtttcc aatttctatc ctcatgttct
     2701 ggcgaacttt tgtgaaacgg taagagttaa accgatggtc aaccaggtcg agttgcatcc
     2761 ctattttgcc caaccagagg cgctggcaac catgaagtat tataacgtgc agcctgaagc
     2821 atgggctccg ttaggtggtg gacgacataa accctttgaa aataatctgc ttcagagtat
     2881 tgcagatgcc catcaaaaat cgatttctca agtcattctg cgttggaata ttcaacgggg
     2941 agtggtcgtt attccgaaat cgacacatca acagcgtatc gaagaaaatt ttgctatctg
     3001 ggatttctca ctgacagaga aagaaatggc acaaattagt tcgcttgatt tgggttatgt
     3061 tggggagtcg gtaaaacatt ttaatcctga atttgttcgt ggttgtcttg ctgtaaaaat
     3121 acatgattga tattaatcat gtattttacc tgagacgaca agaatctttt aacaggggag
     3181 tgatattgat cttcactctg tcatatctcc ggtaatatgg cgtcaggctt catcatcaga
     3241 aagggggcct gacgcctgaa aaagtgaaca acagacagtg ttcggattat caatattcag
     3301 tttttaacgt tgaactggat tcattactgc gttgtttaag aatttcccgc gccgaatctg
     3361 ccattatcat gacagcacct gccagcatca gagtatcttt caataccaag cgaccagcac
     3421 cggataaata agggaaacca tgatgagcgt cacccaatgc gggtacccat gcctccgggg
     3481 tggtgattaa aaatgagagt gttaccaacg gtgtggtaaa tgccatcagc cctcccaata
     3541 aacctaacca gcgattgaca ggattagcca aaaccagcag agcaataatc acctccacga
     3601 cgccaagacc gttggaaaaa ccataggtat tattggccga ttgccatgcc cttgcttctg
     3661 gtttgtattc gccttcgtga gtcagatact gtttatagtc ttccgggtgt tcataaaaga
     3721 acgacattag tggactgttt gcgacgaatg gtgtaatgct gtctgcctcg taagggacaa
     3781 actttaataa cccaatccac ataaaaacaa tcgcaatact cagacgaatc aatgtcaggc
     3841 caattttatc tccccgactt aacaggtgca ggtatttttc cattgtgaac atccttattt
     3901 atacaatact gctgttgcgg ttgaattatt atcaattcgc atcgatgtga ctttccagga
     3961 tgaagcatta tattttctgg ctatatcagc aatggcatcc tccagaactg tcggtgatgc
     4021 tgatactgac gagacgctga tatgtttcag aattatttta tcatctgcat tggtggaaaa
     4081 tgccataacg ccagataata gtgttgcaaa taaaacggat tttttaaaca tataaaatac
     4141 catcaatgaa gtgattaaga agttatagag taacagggaa ttaataagat tcttttctct
     4201 gggacgccag aatatttgtt ctggcgtctg attttgagtt tatttgatta atgaaaatag
     4261 atcattgagt gattcgctca tcgacggatg agtaaatatc tgatcgcgta atatgctata
     4321 aggcagcccg gcatccatca ccattttcac tatattaatc atctcgtggg agtcaacaca
     4381 cagcagtgat gcccctaata tacgttgggt tttattatca acaatcgctt ttaatacccc
     4441 acgggtatcg ttcatcactc tggcgcgcgg aattgcagct acaggcaatg tcaccacctg
     4501 aatatcagca ccactctctc tggcttgttc ttctgtcata ccaaccctgg acaagggcgg
     4561 tgtcataaat acggaataag gcacattttt ccgatcatca gtactacgtt tgccttcacc
     4621 cagtaactca tcacgtacaa tgcggtaatc atccagtgat atgtaagtaa attgcagccc
     4681 gccggtaaca tctcccatcg cccaaatatt gtctgcggtg gtatgtaatc gcttgtcaac
     4741 gacaattccc ccgcgctcgt ttactgcgac accggcattt tctggatgta acgaagcggt
     4801 agccggttga cgaccggaag ctattaacag tgcatccacc gccagttggg cgtgctcgct
     4861 atgcacttgc acttgatttt catggtgact gattcgctcc acatgggcat tgaggataat
     4921 atcgacgccc tgatcgcgta aaatcgtcgc gatattatca gcaatatccc gatcttcccg
     4981 aggcaaaaac agcgaagctg cttctaaaat ggttaccttg ctgccaaaat tagcgaacat
     5041 agaggcgaac tcaacgccaa tatatccgcc gcccaaaata cctaaatgcc caggcaattc
     5101 ttttagatta agtaatccgg tgctgtcata tacaccaggc gtggtggtaa ttccaggaat
     5161 tggcggaacc acggtttgtg caccggtatt aataaaaatt ttctcgccat gaatttccag
     5221 atttccctca ggccgatgaa cacgcaggct atgattattg ataaactccg cctggccgtt
     5281 gatcacgtcg atattgggca tatctgcaag attatgaaaa ttcttattac gtaaaaaatt
     5341 aaccacttca tttttacgct gtatggcacg gacaaaatct gtgtgctgct gtgcgtcatg
     5401 aaccaatgtt ttggttggga tgcagccgat attaatacag gtcccgccat acattgcatt
     5461 tgattgttcg atgagagcca cacgccaacc tgcttttgcc agcgtgacgg ctaatgtttt
     5521 tccagccttg ccaaaaccaa taatcactgc ctgatattta ttcatgatga tttcctgtcg
     5581 aaacgcgttc atttgacatc atgactatag acgcaaaaga gcaaataaca tttcttcaca
     5641 acgcttgata ttttgtctta tcgtctggta ttttcttttt cttaatcaga atcacaaaac
     5701 aaaaatcatg attaactatt tgatggtatt agattagttg ttt
//
LOCUS       DAZGCQ010000214         5578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000214 DAZGCQ010000000
VERSION     DAZGCQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.214"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..219)
                     /locus_tag="ACHK07_005024"
     CDS             complement(<1..219)
                     /locus_tag="ACHK07_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105225.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSR"
     gene            complement(288..1985)
                     /locus_tag="ACHK07_005025"
     CDS             complement(288..1985)
                     /locus_tag="ACHK07_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO8105226.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(2081..3217)
                     /locus_tag="ACHK07_005026"
     CDS             complement(2081..3217)
                     /locus_tag="ACHK07_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8105227.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            3893..4101
                     /locus_tag="ACHK07_005027"
                     /pseudo
     CDS             3893..4101
                     /locus_tag="ACHK07_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            4088..4277
                     /gene="tnpB"
                     /locus_tag="ACHK07_005028"
                     /pseudo
     CDS             4088..4277
                     /gene="tnpB"
                     /locus_tag="ACHK07_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(4280..4468)
                     /locus_tag="ACHK07_005029"
     CDS             complement(4280..4468)
                     /locus_tag="ACHK07_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO8105228.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            5003..5144
                     /locus_tag="ACHK07_005030"
                     /pseudo
     CDS             5003..5144
                     /locus_tag="ACHK07_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(5319..>5578)
                     /locus_tag="ACHK07_005031"
     CDS             complement(5319..>5578)
                     /locus_tag="ACHK07_005031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105229.1"
                     /translation="RVLDPVGTFHIFSDNAFVNPELSNITGMAQEKGLADVICQQDVI
                     FLKKYLFPGNLCFKRTVNKNNVTDNHHTYSLISIKTTISKD"
BASE COUNT         1529 a         1228 c         1112 g         1709 t
ORIGIN      
        1 cctggaacct cgggatttaa cttttctttt tatatttttg ataaaatcat caatagaaaa
       61 ctgaatcgtc tctgataaat actgttgagc tatatctgta ctctgttttt ctaaatcaga
      121 agctttcttt gtttcaagaa cagatttgat taactcctta cgttgtgtac accaaatctc
      181 gtcagatgaa cagtattttt tattttcgtg tttgttcatt atttattgct ctaactgatt
      241 tatttcccta tatccaggga gtagatatat ctactccctg cttattatca tttcacgtta
      301 gttaaagatt tttttatatt ctcaaatttg gcttggttga tatcactgat cggtttttga
      361 cttgacgtga tgaagtcaat cataacagcc ttcatattct taactacatc aggatgggat
      421 gcagagagat ctttagtttg ttgcagatcg ttgatatcgt acagagctag tttattctct
      481 gcataggtgt aaaccagaga ataatcgttg cttcttacag tgtatgcaaa ctggcttaag
      541 tcttctgtat gtgggttgtg cggataatca tcagactctt ttctcacgaa tttatgatat
      601 ccgttccaga atgcagtgtt ctcttcgtcc caccagtgag aataagaggt aatccaggac
      661 agatatttgt gaggttcgcc agctgcagat ttcttatcag ctcctttctg tacatatggc
      721 aataatgata caccatccag tttcagagaa gcagggatct tgatatctgc ggcatctaaa
      781 gcggtcggat agaagtccat tgatgaaacc agtttgtcat aatcacccgt tttcagcttg
      841 ccattccacc acatgaacat aggtgtatgc ataccacccg gatacggttg gcctttgtaa
      901 ccagcctgcg caccattcag tggcagtgga ccatcaatta cagcaccgtt atcagaggtg
      961 aacagaatga tggtgttgtc gtactgacct ttttccttaa gtgtattcag gatcttttta
     1021 accccctggt caacggagta aattgacgca taatagttat cagcagtctt gctaccagtg
     1081 ttgaatttct cctgatagtt tttaggcgca ggctgatcgt taggcaggtg tggtgcactg
     1141 tatgccagat aaatcataaa tggctgttcc agtttgcttg cacggtcaat tacaccaatt
     1201 gcttcatcag ttaattgatc actaatgtac ccttcagctt taatgttttc acggttacgg
     1261 aacaatgacg gggagttata aagtgaaacg ccggatgcgt gatagcccat gaagtagttg
     1321 aagccacgat tctgaggttg ccattcttct gcggagaagg tggtgaagtt atcgtggtag
     1381 tcacgggttt gtttttcttc aggcacacct acgttgctga tttttgataa gtgccactta
     1441 ccgattgctg cagtgtaata accatgattc tgaagtaatt cagacaggaa tgtttcttcc
     1501 agagggatac catcctgtgc atcagaattt gaatatacac cgaagcgagc aggagctcgc
     1561 gccgtcagga ttgcggcacg tgaagggcca gagacaccgt gtgcaacata gccgttagtg
     1621 aattttacac cttcgtccat cagggccgct aatgttggag tggatttttt tgcagcttca
     1681 attgctttat caatacctat tttgtatgta tcaacaacat ttcggttatc catcgtttta
     1741 gggtcaaatg aactatcatc aaaaggcaat tgtccatacc ctaaatcatc cattgttaaa
     1801 acaatgatat ttggcttacc tttagtgctg tattccgtcg gcgtgaagtc cgcaaaggca
     1861 acattggttt ttgttgcttt gagcttaaca tcatctgctg catttgcagt actcatcatc
     1921 agaccagatg ctaatataag tgatattgat gagttaagca gttttttatt taactgatgt
     1981 tccatatggt attccttctg agttgcatca cgaacattta ttaataataa aagcctctga
     2041 tataaataaa aactatgtaa atcgtttaaa tggatacctg ttaaaactga tacatcatac
     2101 caacagcaag tatattatcg gttgcaactc cagccttttt ggtaaattcg ctttcatcaa
     2161 taaggttaat tttgtaatca ataagaagtg acatgttttt gttcagaaag tagtttgcgc
     2221 caacatcaat atactcaaca agagactgat cgccatagtt tactattcca cctttaacat
     2281 ccatcccttt ggactgaacg taagccagtg aaggacgcag gccgaaatca aacatatact
     2341 gagccacaac ttcaatattc cgtgctttat ctgcgatgcc tgcgctacca aatggagtca
     2401 tatttcgggt ttcagagtag tcagctgcca gatacaggtc attatcatta tacttgaccc
     2461 caacacctgc aaactccgca tgttttcccc cggcaatcca gcgaggcgta ataacttctt
     2521 ttccttctga atctttcgat gtcatgaaac gtttacggcc ttctttttcc tgcagactgg
     2581 ttctgtcggc attagtgtaa gcggcaacga ttccaaaacc ttcatagtca tagcttacgg
     2641 agaaaccata tccatcacca ttagcttcaa gtaaattatc acggtcattt ttaccctgat
     2701 attgagcaga aaaagtaagt ccatcaacaa gcccgaagaa atctgtattg cgccaggtca
     2761 gaactccggt tgttctgccg gtcatatagt tatcagagta agtccagctg tcaccaccaa
     2821 agtccggaag tacgtcagtc agagccccca catcataggc aacaccatag tttcgcccat
     2881 aatcaagtga accgagtata ttgtggcgaa tacctgcaaa tgccagacga tttttatcgt
     2941 tgcgtacttc actttctgtc ttaaacccca ggaactggta ttcccactga ccaaagccag
     3001 tcaggctctc attaatttgt gtttcgcctt tgaaaccaag acgtgcaaat gttctgtcgc
     3061 catccataga accatcagag atgtaatgac gggctttaac gcgaccataa aaatccagtt
     3121 tattaccgtc tttattatag atttctgctg cctgtgttgc ctcacacagc attaatgcag
     3181 aaacgacaga gcacacaacc atacttaatt ttcgcataaa aaaatcccct gtgcacattt
     3241 ttagtatggt tttaaaaatt aaactatggc gaaaaaatta tcactatgca aagatgtaac
     3301 aaaatattgc tcagtgatct atgttgttgt ttttatttga ggttgtatat tttctagtgt
     3361 gtttgacttt tctttctcaa gtgttatttc ttgaccttta tcacagaacc atctccctgg
     3421 atatccccat cacattgcat gaaaagcaag ttttaccaat cttgattttc agtgttaaat
     3481 aagattaata actgtaattc tgcaaaaaaa agaacaccat atgtagtatg actacgtatt
     3541 ccactctgtt cgttcacgga ctggcatcga agtctgaggt gtactggcaa tagcggatac
     3601 agtcatttgt tcttttttta agcattcatc cgatgatact tttcctgaat gctaccggag
     3661 tggtattccc ctgccgtgag ggagggcgct gacggttata aaaatcctga tatataccca
     3721 ttcccgtatg acagcgatgg cgtcacatct ctgttctacc atcttcacag aaatatttcg
     3781 ttcattagca ttccggtatt cgtataacca ccttcggtgg agaaagaata aacaatccct
     3841 ggaatgcgat gagcgcttga ccagaaaagc atcatattgc ttcatggcgc atcagcgacc
     3901 acaggatgcc gcaaaagccg aaaaacggac cagagcgcct gcaatcattc ctcgcctcag
     3961 tatctgttgc ccatggagtg gccgataaca gatcattgtc ggatgagcct tatccccttc
     4021 cttcccggtg gtatccggat gtgctgccag ccttctcagt taaccgatgt tttccgggct
     4081 ttttgccata ctgaggactg ataacgtatt cctggtcgta aaacccgtca atatgcgccg
     4141 gggcatcgac aaaactgaca cagtacgtcc ggaaagaact taacgctgca tggcacgaca
     4201 atgctgcctt tgtctttacc aacaaggctc gttcccgcat aaaagttcta cgccgggata
     4261 aacatggcgt atggctgcgt caggccttca ctacttcaca ccggaagtct tccaccctga
     4321 cacgaatata ccgtccgggc tctttcgcag gtgcaaacag tttttcacat tcggcgaatg
     4381 tcatttctga atcctggtaa cgcaaccact gaaccggcat attaccgggt ttgattattg
     4441 tcgcagttat tttatatcgt ttccccatat cgcctctcat cctgacaaca aaattaccgg
     4501 ctgagccatc actccggtaa agcatgcgaa aaaaagtgag cgtctcagca atggctcatg
     4561 cgatgattct gtacgggcac cgatggcacc tcagaataca acaaagatat cagagtaaca
     4621 tgtctctctc gttatttttc agagttcttg acaccctcct tctgccacat tgtcaacgcc
     4681 agtggagact tctgctgtca gttttttcat ccatacagag tgacattaat tatcctgaac
     4741 aaaacgaaat atcctgaata tatttatttt ctcctggtgc cggagacatg atgatgtaca
     4801 ggctcaggtg cggtgtgaat gtaaaatggt atgttaatta ttcatttctg aatgcaaact
     4861 gaatgcaaac ttattccgaa ttgaattctc ctgacaccac atgataaaaa tgaggatata
     4921 ataaaatact tctcttctgg atgtgccatt acctgccgtt gctgtaatac ggcgggtatt
     4981 ttttactgac atatcgggaa gccagcagac gttcgtagct acatccccga aagtggcaca
     5041 ctgcaaacta taatacgagt aaaacatcga tcaccgtagc tgatgtgttc aacgatcgtg
     5101 cactagcgtt ctgcgattct cagggactgc ctgttctgag aatcggcgag tccgcgatcg
     5161 gatacgatgc gatttcgcag tgagtcaggg attgcatctg aaaaccctcc ggctatgtcg
     5221 gtaaatattt atttctatga accgtacgat tcagatagca ttcgcccggc cccgtaagaa
     5281 taatctccgg acaaataaga tttttcctga aaaagcgtct agtcttttga tatcgttgtt
     5341 tttatgctaa tcagggagta tgtgtgatga ttgtctgtta cgttattctt gttaaccgtc
     5401 cgtttgaaac agagattgcc gggaaaaaga tattttttca gaaaaatgac gtcctgttga
     5461 cagataacat cagccagtcc tttttcctgc gccatcccgg taatgttact gagctccgga
     5521 ttgacgaatg cattgtcaga aaatatatgg aaagtgccga caggatccag cactctga
//
LOCUS       DAZGCQ010000215         5251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000215 DAZGCQ010000000
VERSION     DAZGCQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.215"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..1451
                     /gene="garP"
                     /locus_tag="ACHK07_005032"
     CDS             117..1451
                     /gene="garP"
                     /locus_tag="ACHK07_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO8105230.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSGEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1467..2237
                     /gene="garL"
                     /locus_tag="ACHK07_005033"
     CDS             1467..2237
                     /gene="garL"
                     /locus_tag="ACHK07_005033"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO8105231.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2267..3157
                     /gene="garR"
                     /locus_tag="ACHK07_005034"
     CDS             2267..3157
                     /gene="garR"
                     /locus_tag="ACHK07_005034"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8105232.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVVLGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3254..4399
                     /gene="garK"
                     /locus_tag="ACHK07_005035"
     CDS             3254..4399
                     /gene="garK"
                     /locus_tag="ACHK07_005035"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO8105233.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4432..4808
                     /gene="rnpB"
                     /locus_tag="ACHK07_005036"
     ncRNA           4432..4808
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK07_005036"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1240 a         1250 c         1489 g         1272 t
ORIGIN      
        1 tctcgcgctg cttatactaa ttcccgagtt cattgtcgtt acggcataaa tgaaactcat
       61 aaataacacc ttccccataa taataaaaat cagcataagt acccgaggta aataaaatga
      121 ttctggacac cgttgacgaa aaaaagaaag gcgtgcatac ccgctattta atattactga
      181 ttatttttat tgttaccgcc gttaactacg ccgatcgtgc aacgctgtct attgctggta
      241 ccgaagtggc aaaagagttg cagttaagtg cggtttcgat gggttacatc ttctctgctt
      301 ttggctgggc ctacttgctg atgcaaatcc ccggcggctg gctgcttgat aagtttggct
      361 cgaaaaaagt ttacacctac agcctctttt tctggtcgct attcaccttc ctgcaaggct
      421 ttgttgatat gttcccgctg gcctgggcag ggatctccat gttctttatg cgctttatgc
      481 tcggcttctc ggaagcgcca tcattcccgg cgaacgcccg tattgtcgcc gcctggttcc
      541 cgacgaaaga acgtggtact gcctccgcca tctttaactc ggcgcaatat ttctcgctgg
      601 cgctcttttc gcctctgctt ggctggctga ctttcgcctg gggctgggag cacgtcttta
      661 ccgttatggg ggtgattggt tttgtgctga cggcgctgtg gatcaaattg attcataacc
      721 cgacagatca cccacgtatg tctggggaag aactgaagtt tatctctgaa aatggcgcgg
      781 tggtcgatat ggaccacaaa aagccgggca gcgcggcagc aagcggtccc aaactgcatt
      841 acatcaagca attgctctct aaccgcatga tgttgggcgt atttttcgga caatatttta
      901 tcaacaccat cacctggttc ttcctcacct ggttcccgat ttatctggtg caggaaaaag
      961 gcatgtcgat tctgaaagtg ggtctggtcg cctcgattcc agcactgtgt ggttttgcgg
     1021 gcggcgtgct gggaggtgtc ttctcggatt atctgatcaa acgcggttta tccctgaccc
     1081 tggcacgtaa gctaccgatt gtgctgggaa tgttgctggc ttccaccatc atcttatgta
     1141 actacaccaa caacaccacg ctggtggtca tgctgatggc gctggctttc tttggcaaag
     1201 gatttggtgc gctgggctgg ccggtgattt ctgacaccgc gccgaaagag attgttggcc
     1261 tctgcggcgg cgtctttaac gtctttggca atgttgcctc cattgtcact ccactggtga
     1321 ttggctacct ggtaagtgaa ctgcactcct tcaatgcagc actggttttc gtgggatgtt
     1381 cagcgctgat ggcgatggtc tgctacctct tcgtagttgg cgacattaaa cgtatggaat
     1441 tgcagaaata agcaaaggta taagcgatga ataacgatgt tttcccgaat aaattcaaag
     1501 ccgcattggc tgcgaaacag gtacaaattg gttgctggtc agcactctct aacccgatta
     1561 gcactgaagt tcttggtttg gctgggtttg actggctggt gctggatggc gaacatgcgc
     1621 caaacgatat ctccacgttt attccgcagt taatggctct gaaaggcagc gccagcgcgc
     1681 cagtagtacg agtgccgacc aacgagccgg taattattaa gcgtcttctg gatatcggtt
     1741 tctataactt cctgattcct tttgtagaaa caaaagagga agcagagcag gcggtggcat
     1801 cgacgcgtta tccgccagaa ggcattcgcg gcgtctccgt ttctcaccgc gccaatatgt
     1861 ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa catcactatt ctggtccaga
     1921 tagaaagtca gcagggcgta gataacgtcg atgctattgc cgctaccgaa ggcgtagacg
     1981 gcatcttcgt cggccccagc gatctggccg cggcattagg ccatctcggc aatgcatcac
     2041 acccggatgt acaaaaagca attcagcaca tttttaaccg tgccagcgcg cacggcaaac
     2101 ccagcggtat cctcgcgccg gtcgaagccg atgcgcgtcg ttatctggaa tggggcgcga
     2161 cgtttgtggc tgtcggcagc gatctcggcg tcttccgctc tgccactcag aaactggctg
     2221 atacctttaa aaaataacca ccaccgcaac aagagagatg attgatatga ctatgaaagt
     2281 tggttttatt ggcctgggga ttatgggtaa accaatgagt aaaaaccttc tgaaagcagg
     2341 ttactcgctg gtggttgctg accgtaaccc agaagctatt gctgacgtga ttgctgcagg
     2401 tgcagaaaca gcttctacgg ctaaagcgat cgctgaacag tgcgacgtca tcataaccat
     2461 gctgccaaac tcccctcatg tgaaagaggt ggtgctgggt gagaatggca ttattgaagg
     2521 cgcgaagcca ggtacggtat tgatcgatat gagttctatc gcaccgctgg caagccgtga
     2581 aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg gatgctccgg tgagcggcgg
     2641 tgaaccgaaa gccatcgacg gtacgctgtc agtgatggtg ggcggcgaca aggctatttt
     2701 cgacaaatac tatgatttga tgaaagcgat ggcgggttcc gtggtgcata ccggggaaat
     2761 cggtgcaggt aacgtcacta aactggcaaa tcaggtcatt gtggcgctga atattgccgc
     2821 gatgtcagaa gcgttaacgc tggcaactaa agcgggcgtt aacccggacc tggtttatca
     2881 ggcaattcgt ggtgggctgg cgggcagtac cgtgctggat gccaaagcgc cgatggtgat
     2941 ggaccgcaac ttcaagccgg gcttccgtat tgatctgcat attaaggatc tggcgaatgc
     3001 gctggatact tctcacggtg tcggcgcgca actgccgctc acagctgcgg ttatggagat
     3061 gatgcaggca ctgcgtgcag atggtttagg aacggcggat catagcgccc tggcgtgcta
     3121 ctacgaaaaa ctggcgaaag tcgaagttac tcgttaatga cgtcgtgccc ggatggcgta
     3181 ttgcaatccg ggcctggaat ccaggccgaa taagagaaac gccctccggc gtcatgtggt
     3241 aacaggcata ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa gtttatctgc
     3301 cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa atttttcctg atgcacagta
     3361 cgtttctgtt cctgttgccg acggtggcga aggaacggtg gaagcgatga ttgcagccac
     3421 ccagggttcc gaacgtcacg cctgggttac agggccgctg ggcgagaaag tgaatgccag
     3481 ttgggggatc tccggcgatg gcaaaaccgc gtttattgaa atggcggcgg ccagtgggct
     3541 ggagctggta cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg gcacaggcga
     3601 gttaatcctg caggcgctgg agagcggcgc gacaaacatt attatcggca ttggcggcag
     3661 cgctacaaat gatggcggcg caggcatggt acaggcgctg ggggcgaaat tatgcgacgc
     3721 caacggcaat gaaattggtt ttggcggcgg tagtcttaat actctgaatg atattgatat
     3781 ttccggcctc gatccgcgct taaaagattg cgtcattcgc gtcgcttgtg atgtcaccaa
     3841 tccgctggtg ggcgataacg gcgcatcgcg catctttggc ccacaaaagg gagccagtga
     3901 agcgatgatt gttgagctgg acaataacct ctctcactat gccgaggtca ttaaaaaagc
     3961 gctgcatgtt gatgtgaaag atgtccccgg cgcaggagct gcgggtggta tgggcgcggc
     4021 gctaatggcg tttcttggtg cggaactgaa aagtggtatt gaaatcgtca ctacggcgct
     4081 gaatctggag gaacatattc acgattgtac gctggtgatc accggtgaag ggcgtattga
     4141 cagccagagt attcacggca aggtaccgat tggtgtcgca aacgtggcga agaagtacca
     4201 taaaccggtg attggcattg cgggtagcct gaccgatgat gttggcgttg tacatcagca
     4261 tggcattgat gcggtcttca gcgtattgac cagcataggt acgttggacg aagcattccg
     4321 cggggcttat gacaatatct gccgtgcttc acgtaatatc gccgcgacac tggcgattgg
     4381 aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc cgaagctgac
     4441 cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg ggaggaaagt
     4501 ccgggctcca tagggcaggg tgccaggtaa cgcctggggg ggaaacccac gaccagtgca
     4561 acagagagca aaccgccgat ggcccgcgca agcgggatca ggtaagggtg aaagggtgcg
     4621 gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg gtaaactcca cccggagcaa
     4681 ggccaaatag gggttcataa ggtacggccc gtactgaacc cgggtaggct gcttgagcca
     4741 gtgagcgatt gctggcctag atgaatgact gtccacgaca gaacccggct tatcggtcag
     4801 tttcacctga tttacgtaaa aacccgccag cgtaaactgg cgggtttttg cttttggagg
     4861 ggcggaaaga tgaatgactg tccacgacgc tagacccaaa agaaagcggc ttatcggtca
     4921 gtttcacctg atgtacgtaa taaaccgttc cggcgggttt cagactgttg agtgcgcttt
     4981 attcatgccg gatgcggcgt gaacgcctta tcctgcctac aaaagcacgc aaattcaata
     5041 tattgcagag atggcgtagg cctgataagc gtagcgcatc aggcattttt gcggttgcca
     5101 tcagtttcaa acccgcgtcg ataagttttt gcttttggtg cacagggtaa gatgaatatc
     5161 tacggtatta ataatagact ttgtaagaag ttattaagtc tatattgcat ttaaaaggtc
     5221 gtaatcatac gtatagtcct ctctggtaaa a
//
LOCUS       DAZGCQ010000216         5202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000216 DAZGCQ010000000
VERSION     DAZGCQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.216"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK07_005037"
     CDS             <1..207
                     /locus_tag="ACHK07_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO8105234.1"
                     /translation="SSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYN
                     ETHPHSALGYLSPREYRRQRVTST"
     gene            247..726
                     /locus_tag="ACHK07_005038"
                     /pseudo
     CDS             247..726
                     /locus_tag="ACHK07_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            869..1098
                     /locus_tag="ACHK07_005039"
                     /pseudo
     CDS             869..1098
                     /locus_tag="ACHK07_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1085..2587)
                     /locus_tag="ACHK07_005040"
     CDS             complement(1085..2587)
                     /locus_tag="ACHK07_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8105235.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(2749..3510)
                     /locus_tag="ACHK07_005041"
     CDS             complement(2749..3510)
                     /locus_tag="ACHK07_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO8105236.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            4300..>5202
                     /gene="subA"
                     /locus_tag="ACHK07_005042"
     CDS             4300..>5202
                     /gene="subA"
                     /locus_tag="ACHK07_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO8105237.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTL"
BASE COUNT         1485 a         1187 c         1197 g         1333 t
ORIGIN      
        1 agtagccctc agagcaatgg aatggcagaa aggttcgtga aaacgatgaa agaagactac
       61 atcgcgttca tgccgaaacc gaatgtaaga acggcattgc ataaccttgc agtggcgatc
      121 gagcattaca atgaaaccca tccgcatagt gcgttaggtt atctctcacc aagggaatat
      181 cgacgtcagc gggtaacgtc aacttaagat acaacgactg tctgaatatc gtgggtcaag
      241 gacagccttc tgcattttct taatggggaa ctggtaaaag ctgcaactgc atttcgtcgc
      301 ggctttgcgg caaatactta catcgcagaa atactgtgcg gaaatctgca tccatttcca
      361 cttgctgtat ggcataactt ttcaggcggt ccggatactg ctgaagatta ttatgccaca
      421 taccacccgt tatgggggca atatccggaa gcattgctgt ttgtaaactg gctctataat
      481 cattcctctg tgctgcatga acgggcagaa atcattaaat gcgccgaaat gctgatgcag
      541 gaagatgatt tcgaaatatg cgaaagtatt ttaagacagc aggagaagtt gcgtgaaaga
      601 attggtgaga cgctttctga gaaaattgta cagaaatgca gaaatatgaa tggtgaatat
      661 gtctggccct ggatattgcc gttttcagcg gcaggcatga aacatactgg catacagtat
      721 cagtagatat tgcattagtg tatcctgcac acaagtaata atttatccac caataataac
      781 actgttaatg tccccttccc ctggttgtca gccaggggtt atcttctgaa tatttctttt
      841 gaaaaggata acacagtaaa ttatttttat gaattatccc atggactcat taacaccctt
      901 tcataatgct ttattgtcaa acacgttatg gctgacatca aaaaaaccgg atttcctctg
      961 ccagcgggta atcacctccc cggtgttttc ggttggtctg gttactccag tctggtaatt
     1021 agcaagataa tcgatataaa cagtggaaaa ctcatcgtac ataatctggt gatgaacatt
     1081 acgcttattt tcccctgacc ggaagaatca gaggctgcgg tttcagactg tctgccggta
     1141 cattcctctc cccgttaaaa accataacgg gttcattatc ttcgtctgtc agcggattga
     1201 atggcggtat attttcagta cgaatgccgg tcagccactg aaaaatacct gcgaaatgac
     1261 gggcactgat tttcctgctg acggactgat gagacgtgat gtcactggcg gtaataatca
     1321 ggggaacgct gtagcctccc tgcacatgac catcatgatg aacaggattg gcactatcgc
     1381 tgaccgacag cccatggtca gaaaagtaaa gcatggcgaa atgacgggaa tgccggcgaa
     1441 ggataccatc aagctgaccg agaaagttat ccagtttact gatgctggcg aggtaacagg
     1501 caatctttcg gggatactgc tccaggtaat gattcggcca ggagttaagt cggtcacacg
     1561 gattcggatg agagccaatc atgtgcagga aaatcacctt cggagaggat ttatccgcca
     1621 gcgcacgttc tgtttcctgt aacaacaaca tgtcatccgt tttacgggaa gcaaagctgc
     1681 ctttcttgag gaaaacggta tgctccgcat cagaagcaat aacagagatg cgtgtatcat
     1741 gctcccccag ttttccctga ttggatatcc accatgtgct gtatcctgct tttgctgcca
     1801 gcgctaccac gttgttgccg gagtcagggt tctgctcata gtcataaatc agtgtccggc
     1861 tcagggaagg tacggtactg gctgctgccg atgtatagcc gtcaataaat aaaccgggag
     1921 ccgtattcag ccacggtgtg gttggtacgg gataaccata taccgacata taatccctgc
     1981 gcacactctc accagtgaca atcacaatcg tgtcatacaa cggtgctccc ggcaggattt
     2041 tccagctgtc agccctgtgc tgactcagtt gttcataacg ctgcatttca cgcaatgtgt
     2101 cagttgtccc cacaacggtt cctttaacca tccgcaacgg ccagctgttt acagagcata
     2161 atacgaacag cagcagtgcc agccagttac ggtgaccgcg gtggtgtgtc cgccagaaaa
     2221 taaccatgaa caccagaatc gcggcactga ccagaaaatg ataaacagga atcatcccgg
     2281 taaactccgc agcctcatcc gccgtggtct gcaacaacgc gacaataaaa ctgttgttga
     2341 ttttaccgta cgtcataccc gcaggcgcat acagtgcaca acagaacaga aataacagag
     2401 ctgtaatgga tgtaagggta tttctgtgtg caaggagcag aagaaggaac agcagcaaca
     2461 cattcccggt ggcattcttc tcagtgtatc cgcatgcaat tgtggttatg acagaaataa
     2521 caaaaaagaa taaaaacaat aaaatccgtg gtgtgttgtc cggacaaaac agttttctga
     2581 tattcatcgg agtatatcgg caacattatt atgaagagaa taaaataata agaaaaacag
     2641 aaatatcctg taaagcagat aacagacaac cctgcagtat aattacggca gggagtttcc
     2701 gtttaattac agaagacagg taattattat ctttcaggag tagtttcgtc agaagttata
     2761 agtcacacca agcatgatgt catgactttt aacatcaact tctgatttat atttatcgcc
     2821 ccactcatcc ttgtaactca cactggcatc acctgcatca agatagcgat agctgaggtc
     2881 cagagcgata tccggggtta cgtcatagcg gacacctgcc ccaaaactcc atgcaaaatt
     2941 gttatcatat cccgggtaat gcaacgcaga aaaattgctc acctcaccag atttattcca
     3001 ggaggtatca gtatatgttg ccttatgatg tatccgtgca taacccagtc caacagacac
     3061 ccatggagtg aatgcactgc tggtgaatgc actgctgttt ctgaagtcat aatacgtatt
     3121 caccatcaga gtattcacac tgagcctgtt ctgtgtgtct tcccgaccac caccgaccat
     3181 cggagagtac catgtatcca gtgtataacg ggagtccgca gctccgcggc catagaattc
     3241 cacttccgtg cgcacaggaa cattgtaatg ctgataaaaa tcatagccca ccgcaaggcc
     3301 ggcaccgaat accgtatcgt tcttatcgcc gcccttatac ttataaacat cgtccccaaa
     3361 gtcctgacga aagcgctggt cagtctgagt cacaaccgaa gcaccagctt taccggtcat
     3421 ataaaagcct gttttgcctc tatcagccag ggccaggcct gaaaacatgc ccaccacagc
     3481 aagcgctgaa accgcaataa tctttttcat ttcagttatc tccgctatca ctttccccga
     3541 acgataaacg tggttgctta ttctaaagct atgcaaaaga cagggtaaaa agagaaaaat
     3601 cgatcgaatc gatcgacaaa atcgatcatg catgatgaag atttcttatc gaatacataa
     3661 aaatagtgat ggcaatcgtg gcaggagctt acgtcaggcc ctgaagtact gacacaggca
     3721 ttgatggcag gtgatataag gagtgctcca gggaatgact cccgactgac ccaggcactg
     3781 tcccgttgtc aacccgtatg gttactgctc tgactcattt cctgtcgtca gacgttcaac
     3841 tctggaccac tggttatgac gacaggtgtc gctcatttgt cagcactata acggtcaaag
     3901 atcgtggcat tgacacattc tccagaggta aatttctcaa gaggtaacaa ctctgccagg
     3961 aggatatctg cagagagtta cattcgtttg ggttcatatc tgtgccgcgc gttttccttc
     4021 catcagtgaa caaacattca gtcccatgac gaccgggcaa atagcgtgtt cacagatatt
     4081 gctgtccagg tacgaagtac cataatggat attggtaagt tattgggtga accccctcca
     4141 tgaaaaaacc aaatataaaa atgaatgttt cgggagagac ttcactcgct ttattgatag
     4201 tgattgttta tcttttactg aagacaggat atttcagatg tgatatccct gtaacatatt
     4261 gaccagcaat actttttatt tgtaccactg gaggatattg tgctgaaaaa tttacggctg
     4321 catattctgt tcctgctttt tatctcagtg tcagtcaggg cggaaaaacc atggtatttt
     4381 gatgcaattg gtctgacaga aacaaccatg tcgctcacag acaaaaatac cccggttgtc
     4441 gtatctgttg ttgattccgg agtggcattt gtaggaggcc tcagcgacag cgaatttgcc
     4501 aaattcagtt ttactcagga tggttcgcca ttcccggtaa aagagcctga agctttatat
     4561 attcatggta ctgccatggc ttcactcatt gcctcacgtc atgaggttta tggtgtttat
     4621 cctcatgcac tgatatccag cagaagggtt attcctgacg gtgtacagga ctcatggatt
     4681 agagcaactg aaagcattat gtcaaatgtt tttcttgcac caggagaaga gaaaatcatt
     4741 aatatatcgg gaggccagaa ggggatatct tccgcatcgg tctggtccga actgctttcc
     4801 cgtatgggcc ggaataatga gcgattaatt gttgcggcag tgggtaatga tggcgcagat
     4861 atacgaaaac tgagtgctca gcagagaata tggcctgcgg cttaccatcc ggtcagcagt
     4921 gtgaataaaa agcaggatcc tgtgataaga gtggcagctc tggcacagta ccggaaagga
     4981 gaaacacctg tattgcatgg tggaggggtc accggaagtc ggttcggaaa cggctgggtt
     5041 gatattgccg caccgggaca gaatattaca ttcctcagac cagatggaaa aacggggatc
     5101 ggtagtggaa cctcggaagc aacagctata gtttccggcg ttctggcagc gatggtctca
     5161 tgtaatcccc gtgcaacagc gacagaactg aagcgtacac tg
//
LOCUS       DAZGCQ010000217         5199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000217 DAZGCQ010000000
VERSION     DAZGCQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.217"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..141
                     /locus_tag="ACHK07_005043"
     CDS             <1..141
                     /locus_tag="ACHK07_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8105238.1"
                     /translation="IGRPLTGIFCLVLQITLLGWIPATIWAVYALSQYKTEQKIREAT
                     GK"
     gene            285..1130
                     /gene="mscS"
                     /locus_tag="ACHK07_005044"
     CDS             285..1130
                     /gene="mscS"
                     /locus_tag="ACHK07_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8105239.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1256..2476
                     /locus_tag="ACHK07_005045"
     CDS             1256..2476
                     /locus_tag="ACHK07_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO8105240.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2707..4452
                     /locus_tag="ACHK07_005046"
     CDS             2707..4452
                     /locus_tag="ACHK07_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8105241.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMAVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4634..>5199)
                     /locus_tag="ACHK07_005047"
     CDS             complement(4634..>5199)
                     /locus_tag="ACHK07_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8105242.1"
                     /translation="RIDAINKIKIELHRSSPFYAEPVDCVIWIKNNLIEANDYNPNVM
                     APSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPV
                     TCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTD
                     EVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1301 a         1010 c         1360 g         1528 t
ORIGIN      
        1 atcggccggc ctctgacggg gattttttgt ctggtgttac agataacgct gctgggctgg
       61 ataccggcaa caatatgggc cgtgtatgcg ctttcgcagt ataagacgga acagaaaatc
      121 cgcgaagcca cggggaaatg atgactgcag aagagttggt tgtcataccg gtgtatatga
      181 tgcgggataa aggcccggag attcctggaa ataaaatgtt ccggggaata taaaacaggg
      241 aaaaaccgga tgtgatggac gcttctggcg aggcggtttc atgaattgtc cgtaatcagg
      301 atatgttgcc cggttacgcc gcgaatcttg tggcagcagt ggttattatt atcgggggta
      361 tcgctgcacg gatgatttct ggtgtgctga ataatctgat gctgggcagg aatattgatg
      421 ccacagtggc gctgttcctt tccgcgctga tacgtcatgc cattatagcc tttacgctga
      481 ttgctgccct gggacgtgtg ggggttcaga cagcttccgt aattgcagtc ctgggggctg
      541 caggtcttgc tgttggactt gccctgcagg ggtcattgtc aaaccttgca gctggtattc
      601 ttctcgtgat gttccgtcct ttcagggctg gtgaatatgc tgacctgggt ggtgttgccg
      661 ggactgtgca gaatgtacat atattctcaa ccacgatgcg tacactggat ggtaaaattg
      721 ttgtcattcc gaacgggaag attattgccg gagagattgt taatttctcc agggagcctg
      781 aacgacgaaa tgagtttatc atcagtgttt catatgatgc agatattgac tgtgtgaaac
      841 aagtcctgac agatattgtg atgtcagaag agagggttct gaagaacagg gaaatcactg
      901 ttcgcctgaa tgagcctggg gagtcatcca tgaattttgt agtacgggta tggagcaggc
      961 gtgatgacct tcagtctgta tactgggatg tactggagag aattaagcgg gagtttgatt
     1021 cagaagggat aagttttcca tatccacaaa tggatatcca tcttgttcgc tccgggaagc
     1081 aggcagagtc ggtaacccat aacagtcgct cagtcagttc cagacagtaa aatgaaaagc
     1141 gcattttatt tgtgaaagat gaacagcctg aatctgtcag gctgttttca ttgtgttttt
     1201 atggtcattt ttggtatatg tactgtatat aatgtcatct gtacaaggat ataaaatgcc
     1261 aaagaaaata atgacaggga ttgatgtttt tgacgctgcc atggagcgta ttgaatgggt
     1321 gtttgatacg ttttcatcag tttgtctttc tttttccggc ggaaaagact ccactgtcct
     1381 gtttcacctg atggcgaata ttgcccgccg aaaaaaacgc cgtttttctg tactgtttgt
     1441 tgactgggag gctcagtatc agtgtaccat tgagcatgtc accaggttga gggaaatgta
     1501 cgatgatatc acagatactt ttttctgggt ggcgctgcct ctgacgaccg ttaatggagt
     1561 gtctcaggta cagcctgagt gggtatgttg ggaggacggg gctgaatggg ttcgccagcc
     1621 accagaagat gccataacca gagcgtcttt ttttcctttt tatcagaatg cgatgacatt
     1681 tgaagagttt attccggcat ttaatcattg gtttgcccgg gaaaaaggga tggttgctgc
     1741 gattcttact ggtgttcgcg ctgatgagtc actgaatcgt tttatgggac tggtatccgg
     1801 acgtaagctc cgttatgcgg aggataaacc ctggaccacg gcatcggctg agggatttta
     1861 ctacaccatg tatcctttgt acgactggaa aacaagagat gtatggattt accatgcgag
     1921 aacacgtgct gcctataact ctctttatga cctgatgtac agggctggag ttcctcttcg
     1981 caatatgcgg gtgtgtgagc cttttggacc ggagcaacgt aaggggttgt ggctttatca
     2041 tgtactggag ccggatacat ggggcagaat gtgtgctcgc gtatcaggtg ctgtaggggg
     2101 agcgctctat gcaaatgaaa gtggtgctta ttttgctttg aggaaacaga taacaaaacc
     2161 agttcaccat acatggcaga gttatgcaat gtttctgctg gagtcaatgc ctgagaaaac
     2221 ggcggagcat tacaagaata agattgctgt ttatcttcgg tggtggcagt ctcatggatg
     2281 gccggatggc atccctgatg agcaggataa tgaccaggga tgtaaagata ttccctcctg
     2341 gcgtcgcatc tgtaagacgt tactgaaaaa tgatttctgg tgtcggatgc tggcgtttag
     2401 tccgaataag ccccgacatt atgagcgcta cctggccaga atgcgtgaaa aaaggaaaga
     2461 gtggggaata ttgtaaatta tatttaaaca tcagaaatat gacggttaat ccaggatagt
     2521 agctaaaaaa ataagattcc ttgctgattt atccctgttt gatatgtaaa atctgagggg
     2581 cttataagca acggatatac gagtgctaca atatatgata agttatatat attaaatgtt
     2641 catcatctgt cagggggctg ttttatggct aaactgataa atatatcagg tgttaccgag
     2701 gagcggatga ctgccgggga aaagcgggtt gcatcccgtc ttgaggcatt tatgagtaat
     2761 gagtgtctgg tctggtacga tattccggtg ggacggaaaa accgacatcc tgattttgtc
     2821 attattgacc cggagaatgg tctggtattt cttgaagtca aggactggac cgttcacacg
     2881 ttgcatcagg taaatcacga acaggtcatt cttgaaacta acggtattct taaatcagaa
     2941 ataaatccgc tgctacaggt tcgtcgttat gcctgtgata ccgtaaatgc tttacctgca
     3001 gactcacgtt tgcgacagaa tgatggtcag tataaggggc gtctgaatct ggcctgggcg
     3061 tatggtgttg tgtttacccg tattacccgc cagcaactga aggctctggc tgggaatgat
     3121 gaaaatgctg tggaaaggat tttcccttca gcgcagacaa tctgtcagga tgatatgact
     3181 cagtctgttt tacctgatat attccgccaa aaaattgccg ggatgtttac gatgggattc
     3241 agaacccggg tgacaccaca ggttagagat attctgcgtg ctcatctgtt tcctgaaatg
     3301 gccgtcagac agcactcaca aatcaaaata atggatattc agcaggagat tctggccaga
     3361 aatattggtg atgggcatcg tgttattcat ggggtagccg gctctggaaa aaccatgatt
     3421 cttttgttcc gttgtcttta cctggctgaa actacccctg gtaagattct ggtgctatgt
     3481 tacaacatta ctctggccag ctacctgcgg gaatgtattg agtccagggg actgaagtca
     3541 cgggttacgg tattccattt tcatagttgg tgcgcctcaa tgataaagcg ccatggaatt
     3601 caggtgactg ctggaggaaa ggattacccg gagaaatgct tcagtgcgct ggaagatgcc
     3661 gtgaacagcg gaacaattac tgatactgga tacgatgccg tacttgtgga tgaggggcat
     3721 gattttgaga gtcgctggct ggctctgata gcccgcctgt ttgataacgc gtcccgttca
     3781 ctgctcctga tgtacgatga cgcacagtcc atttacagac gggaacgggc tctgaacttt
     3841 tcgcttgcca gtgtggggat tcaggcgcag ggacgtactt ccgtcctgcc ggtaaattac
     3901 cgtaatacga aacgcattct gcactttgct tatgcattct cccgtgagta ttttgagaag
     3961 catcagaacc gtgaaatacc gtttgttcag ccacaggcgg gcggtgaaga gggcacagag
     4021 ccggaaattc tgcgttgtgc ttctgagagt gatgaggcag tgcaggttgt gggctggctg
     4081 gagaaacgct atacactgtg cggtcactgg agtgatatgg ctgttttgtg tcctgcagag
     4141 ttttcagtga aacatctgaa agaggtcatg acacaacgcg ggatacctta tgccacctgc
     4201 tttgacagtg aaggtaagaa gaaatattcg cgacgcaagg atgttgtgca tttactgacc
     4261 taccagagca gtaagggact ggagtttccg tatgttgcgg ttatcaatgc ttctttcgtt
     4321 cactcaggtg ctgcggatga gtctgaagtt attccggcgc tttatgttgc gttcacccgg
     4381 gctacacggg aattactggt gacctgttat cgtgaaaaca gtatcagtcg tcatcttgag
     4441 gattttgtat aagttgtgtt ttattattgc tgatatgtaa tcattcgttt tttgataatc
     4501 taagatgggt atttagcgag tattcagagt tgaattaata ttccccatgt ttttgatctg
     4561 tcaatagctg tccattacat ggggtgatca tatatcagtc ggtaattact ggataaagtc
     4621 accccctaaa tgatcacttt attgtccatg ccggactaaa gttttcctcc tcaaatagtt
     4681 ctgttaaccc acttatttgt tttaatctca gtacctcatc tgtatccatg cccagttcac
     4741 tacctattcg gagatcatcc catcccatcc gggaaagatc tcggacgata tcggagagcg
     4801 cgtctatctg gtgctttcct ctggccctgt tatgcctgat ggtcgaggcg atagagtaag
     4861 ctttaaagtt attatcatgc ttaatgcatg tgacaggtaa atatcctttt agttttttct
     4921 ctagttttgt tgtgcttttc ccaagcattt gtctgtgaaa tccgtcaata acggtgtatt
     4981 tccccttttg agctaaggtg acaacaggtt gagtgaatcc atcaaccaac agggagtgtg
     5041 ttaataacct tttttcggag ggagccatga cgttaggatt atagtcgttt gcttcaatta
     5101 gattgttttt tatccatata acacaatcaa caggttctgc gtaaaaagga ctactcctat
     5161 gtaactcaat ctttatttta ttaattgcat caatgcgtt
//
LOCUS       DAZGCQ010000218         5054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000218 DAZGCQ010000000
VERSION     DAZGCQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.218"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..815
                     /gene="sdiA"
                     /locus_tag="ACHK07_005048"
     CDS             93..815
                     /gene="sdiA"
                     /locus_tag="ACHK07_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO8105243.1"
                     /translation="MQDKDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(883..1107)
                     /gene="yecF"
                     /locus_tag="ACHK07_005049"
     CDS             complement(883..1107)
                     /gene="yecF"
                     /locus_tag="ACHK07_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO8105244.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1094..1270)
                     /gene="yecU"
                     /locus_tag="ACHK07_005050"
     CDS             complement(1094..1270)
                     /gene="yecU"
                     /locus_tag="ACHK07_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO8105245.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            1566..2222
                     /gene="uvrY"
                     /locus_tag="ACHK07_005051"
     CDS             1566..2222
                     /gene="uvrY"
                     /locus_tag="ACHK07_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO8105246.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            2219..4051
                     /gene="uvrC"
                     /locus_tag="ACHK07_005052"
     CDS             2219..4051
                     /gene="uvrC"
                     /locus_tag="ACHK07_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO8105247.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            4108..4656
                     /gene="pgsA"
                     /locus_tag="ACHK07_005053"
     CDS             4108..4656
                     /gene="pgsA"
                     /locus_tag="ACHK07_005053"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO8105248.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            4808..4883
                     /locus_tag="ACHK07_005054"
     tRNA            4808..4883
                     /locus_tag="ACHK07_005054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4841..4843,aa:Gly,seq:gcc)
     gene            4938..5011
                     /locus_tag="ACHK07_005055"
     tRNA            4938..5011
                     /locus_tag="ACHK07_005055"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4970..4972,aa:Cys,seq:gca)
BASE COUNT         1281 a         1150 c         1304 g         1319 t
ORIGIN      
        1 gaataatata aattatatat aaatcttatt tatgtgatag tttgaattat cattataaat
       61 gatactcact ctcaggggcg ttgcggttta ctatgcagga taaggatttt ttcagctggc
      121 gtcgcacgat gctgttgcgt tttcagagga tggaggccgc agaagaggtc taccatgaaa
      181 tagagcttca ggctcagctg ctggagtacg attactattc gttatgtgtt cgccacccgg
      241 taccattcac gcgacctaaa gtggcttttt acaccaatta ccctgaggcg tgggttagtt
      301 attatcaggc aaaaaacttt ctcgcaattg atccggtgct gaaccctgaa aactttagtc
      361 agggccattt aatgtggaat gatgacttat tcagcgaagc acagccgtta tgggaagccg
      421 cgcgcgcaca tggtttacgc cgcggtgtca ctcagtattt aatgctgcca aaccgggcgc
      481 tgggcttttt gtccttttcc cgttgcagcg cgcgcgaaat acccattctt agtgatgaac
      541 tgcaattaaa aatgcagtta ctggtgcgcg aaagtctgat ggctctgatg cgtttaaatg
      601 atgaaatagt gatgacgcca gagatgaatt tcagcaagcg cgaaaaagaa attctgaggt
      661 ggacggcgga agggaagaca tcagcagaga tagcgatgat tttgtcaatc tcggagaata
      721 cggtcaattt ccaccagaaa aacatgcaga aaaaaattaa tgcaccaaat aagacccagg
      781 ttgcctgtta cgcggccgct actggcttaa tttgatctct tttctgtcct gcgtgccaga
      841 tgcaaaaacc ggctgaaagg cacgctatca gccggtttta tattactgac ggtaggcttg
      901 tttaatttgc ttaaccgttt tggaaaatac tgcagcctgg gtttcgtctt cgatcagcgc
      961 aagctgtttt tccatcttta acatcacgcg gcccgcgtca gcttgtccca tcgcctgcag
     1021 catcagcgtc agcatcgctt tcaggcagga gacttcattt gccagttctt gattattctc
     1081 ggcagtagaa aaatcaggcg tactcattta ttttcctcgt catgttgcaa tgaaaatttg
     1141 cggtgaaaaa tgttaaggcg gcggagtata ccataagctt tgctaaaaat agcagtggtt
     1201 gttttttgag cgtgatatca acagtactat aaaatacgtt aatataatga ccaataaata
     1261 tttttatcat gaatgttttt tgcccgtatt gttcgggtta attaatgtta catattcagc
     1321 gggctgattt tcatttttgc tgaataaagt caatttttgt cacatttcat cgcagggctt
     1381 actgtgaaac gatccctgaa gccgttggtg acgggcgtga ccataactgt ggacaatcga
     1441 atttacaaaa acgagagaaa aatcgaatac ccaccatttt taacgtttca aagttgcaat
     1501 aaaaaccgct aatatacgaa tgactaacta tcagtagcgt tatccctatt tctggagata
     1561 ttcctttgat caacgttcta cttgttgatg accacgaact ggtgcgcgca gggatacgac
     1621 gcattctgga agatataaag ggtataaaag tcgtcggtga ggcatcgtgc ggtgaagacg
     1681 ccgttaagtg gtgccgggca aatgccgttg acgtggtgct aatggacatg agtatgccgg
     1741 gcattggcgg tcttgaggcg acgcgtaaaa tcgcgcgttc cacagctgat gtcaaaatca
     1801 tcatgcttac cgtccataca gaaaaccctt taccagcgaa agtcatgcag gccggtgctg
     1861 cgggctacct cagcaaaggc gcggctccgc aggaagtcgt gagtgcgatc cgttctgtct
     1921 attcagggca gcgttacatt gcttctgaca tcgctcaaca aatggcgtta agccagatcg
     1981 aaccagaaaa aacagaaagc ccatttgcca gtttgtctga acgtgaattg cagattatgc
     2041 tgatgatcac caagggccag aaggtcaatg agatctcaga acagctcaat ctcagtccga
     2101 aaacggtgaa cagctaccgc tatcgtatgt tcagtaaact aaacattcat ggcgatgttg
     2161 agctgactca cctggcaatt cgccatggtc tgtgtaatgc ggagacatta tcaagtcagt
     2221 gagtgatcag tttgacgcaa aagcgttttt aaaaaccgta accagccagc caggcgttta
     2281 tcgcatgtac gatgctggtg gtacggttat ctatgtcggc aaagcgaaag acctgaaaaa
     2341 acggctttcc agctatttcc gtagcaacct cgcttcgcgc aaaaccgaag cgctggtcgc
     2401 ccagatccag caaattgatg taacggttac tcatacagaa accgaagcgc tgttgctgga
     2461 acacaactac atcaaactct atcagccgcg ttacaacgtt ttgctacgcg atgataaatc
     2521 atatcctttt atcttcctga gtggtgatac ccacccgcgt ctggcgatgc atcgtggtgc
     2581 aaagcatgcc aaaggtgaat atttcggccc gttcccgaat ggctatgccg tacgtgaaac
     2641 actggcgcta ctgcaaaaga ttttccccat tcgccagtgc gaaaatagtg tttatcgcaa
     2701 tcgctcgcgt ccgtgtctgc aatatcagat agggcgctgt ctgggaccgt gcgttgaagg
     2761 actggtgagt gaagaagaat acgctcagca ggtcgagtat gtgcgcctgt ttttgtctgg
     2821 caaagatgat caggtgctta cgcaactcat tagtcgtatg gaaactgcca gccagaatct
     2881 ggagtttgaa gaagcggcac gtattcgcga ccaaattcag gcggtgcgac gcgtcaccga
     2941 aaaacagttt gtttccaata ccggcgacga ccttgacgtt attggtgtgg cgttcgatgc
     3001 gggcatggct tgtgtccacg tattgttcat tcgtcagggc aaagtgcttg gcagccgcag
     3061 ctatttcccg aaagtgcctg gcggtacgga actgagcgag gtggtggaaa ccttcgtagg
     3121 acagttctat ttacaaggca gccagatgcg caccttaccg ggtgagatcc tgctcgattt
     3181 taatcttagc gataaaacgc tgctcgccga ttccctttca gaactggcgg gacgcaagat
     3241 taatgttcaa accaaacctc gtggcgatag ggcgcgttac ctgaaactcg cgcgcaccaa
     3301 tgcggcgacg gccttaacca gcaaactttc gcagcaatct accgttcacc agcggcttac
     3361 agcacttgcc agcgtgttga aattgccgga agtgaagcgg atggagtgct ttgacatcag
     3421 ccataccatg ggtgaacaaa ccgtcgcttc ctgtgtggtg tttgatgcta acggcccgct
     3481 gcgtgcggag tatcggcgct ataacattac tggcatcacg ccgggcgatg attatgcggc
     3541 gatgaatcag gtgctgcgtc ggcgttatgg taaagccatc gacgacagta agatcccgga
     3601 tgtgatactt atcgacggcg gcaaaggcca gcttgcgcag gcgaaaaatg ttttcgccga
     3661 actggatgtc tcatgggata aaaatcatcc gctgctactt ggcgttgcca aaggagcaga
     3721 tcgtaaggct gggctggaaa cgctgttctt tgagccggaa ggtgagggat tcagtttgcc
     3781 gccagattct cccgcgctgc atgttatcca gcatattcgc gatgaatcac atgatcacgc
     3841 gattggcggg caccgtaaaa aacgggcgaa ggtcaaaaat accagttccc tggaaaccat
     3901 tgaaggcgtc gggccaaaac gccggcaaat gttgttgaaa tatatgggcg gtttgcaagg
     3961 tttacgtaac gccagcgtcg aggaaattgc aaaagtgccg ggtatttcgc aaggtctggc
     4021 agaaaagatc ttctggtcgt tgaaacattg atgtctctgt agcaacatag gggtaatctt
     4081 actgacaaca gatagttacc cgtcattatg caatttaata tccctacgtt gcttacactg
     4141 ttccgtgtca tccttatccc attctttgta ttggtctttt atctgcctgt cacctggtcg
     4201 ccgtttgccg ccgcgctcat tttctgcgtc gcggcggtga ctgactggtt cgatggtttt
     4261 ctggcacgcc gctggaacca gagtacccgg tttggtgctt tccttgaccc tgtagcagat
     4321 aaagttctcg tggctatcgc catggtgctg gtaaccgagc attaccacag ctggtgggtg
     4381 accttaccgg cggcaacgat gatcgcccgt gaaattatta tttctgcgct acgcgaatgg
     4441 atggcggagt tgggtaaacg cagtagcgtg gctgtctcct ggattgggaa agtgaaaacc
     4501 actgcccaga tggtggcgtt ggcctggctg ctgtggcgtc cgaacatttg ggttgagtac
     4561 gccggcattg cacttttctt tgtggctgcg gtactgactc tgtggtcaat gttgcaatat
     4621 ttgagcgctg cgcgtgcaga tttgcttgat cagtgatcgt ttcggcgcaa ttttcagcaa
     4681 acgatcaaaa gtggtgaaaa atatcgttga ctcatcgcgc caggtaagta gaatgcaacg
     4741 catcgaacgg cggcactgat tgccagacga taataaaatc aagtgattaa ctgattgctt
     4801 gatgaatgcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag
     4861 ttcgagtctc gtttcccgct ccagtttaaa agacatcggc ctcaagcgga tgtctggctg
     4921 aaaggcctga agaatttggc gcgttaacaa agcggttatg tagcggattg caaatccgtc
     4981 tagtccggtt cgactccgga acgcgcctcc actttcttcc cgagcccgga tggtggaatc
     5041 ggtagacaca aggg
//
LOCUS       DAZGCQ010000219         5047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000219 DAZGCQ010000000
VERSION     DAZGCQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.219"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..753
                     /locus_tag="ACHK07_005056"
     CDS             <1..753
                     /locus_tag="ACHK07_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105249.1"
                     /translation="KTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLN
                     NRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQ
                     VEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTF
                     SVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDA
                     PVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            829..5037
                     /locus_tag="ACHK07_005057"
     CDS             829..5037
                     /locus_tag="ACHK07_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO8105250.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSSFSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTYPDGLRSSREYDEKGRLAAETSRSGETTSYSYDDPASEL
                     PTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRYGQRTAVHQEEGLSIYSLY
                     DSRGRLTGQRDAQGRETRLEYSAAGDLTVIIAPDGSRSETQYDAWGKAISTTQGGLTR
                     SMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQSEDE
                     GLITLWHYDASDRITHRTVNSDPAEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDKGR
                     LTDERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKL
                     GGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLTGQLQSQHLNLPQLDRDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHR
                     LVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTIQTGTTRIQTVYEPGSFTPLIRIETENGEQAKARHRSLAEVLQEDTGVTLPAEL
                     SVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGL
                     PQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGLYYNRNRYYD
                     PLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDILFTGGSGPDN
                     KILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRPSRTAEEEVQ
                     AQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWPGKHADEIKN
                     VKASLKNTLIDVKKRCMPTSGIPR"
BASE COUNT         1212 a         1270 c         1706 g          859 t
ORIGIN      
        1 aagacgcaga tgacgatccg ctcgaaaacc tataagggca gcgggtttaa tgaactgaag
       61 tttgacgatg cgacagggaa agaacaggtc tacatccacg cgcagaagaa catgaacacc
      121 gaggtgctga ataaccgcac cactgatgtg ataaacaacc atgccgaaaa aataggtaac
      181 aaccaggcga tcaccgttac caataaccag atccagaaca ttggcgttaa tcagatacag
      241 acggttggtg tcaaccaggt ggaaacggtg ggcagtaacc agattatcaa agtgggatca
      301 aaccaggttg aaaaggtggg gatcattcgt gcgctgacgg tgggtgtggc ttaccagacg
      361 acggtaggcg gcattatgaa tacctcggtg gcgttgttgc agtcctcaca ggtagggctg
      421 cataaatcac tgatggtggg aatgggctac agcgtcaatg tggggaataa cgtcaccttc
      481 tcggtgggca agacaatgaa ggaaaacacc ggacaaacag cagtttattc tgccggtgag
      541 catcttgaac tctgctgtgg taaggcaagg ctggtgctga cgaaggacgg aagcatattt
      601 ctcaatggta cgcacattca tctggaaggg gagtcggatg tgaacggtga tgcgccagtg
      661 attaactgga actgtggtgc cacacaacct gtaccggatg cgcctgtgcc gaaagattta
      721 ccccccggaa tgccggatat gcggcaattt tgagtcaggt gcaaaaaatt aaaacggaac
      781 tggtgcagtg accagataaa ccatattaaa agcagaagga attgattcat gggcggaaaa
      841 ccggcagcgc ggcagggtga tatgacccgc aagggactgg atattgtgca gggttcagcg
      901 ggggtgctga taggtgcgcc gacgggagtg gcctgttcgg tatgtccggg agggattacc
      961 tatgctaacc cggtgaaccc gctgctgggt gcgaaggtgc tgccgggcga gacggacctt
     1021 gcgctgcccg gcccgctgcc gtttattctt tcccgcgcct acagcagcta ccggaccaga
     1081 acacccgcgc cggtgggggt gtttggtccc ggctggaaag cgcccttcga tatccgctta
     1141 cagatacgcg atgaaggcct gatactcaac gacaacggcg gcaggagcat tcactttgag
     1201 ccgctgtttc ccggcgagat aagctacagc cgcagcgagt cactctggct ggcgcggggc
     1261 ggggtggcgg agcagcacag ttcgcagccg ctaagcgcac tctggcaggt actgccggaa
     1321 gatgttcgcc tgagtccgca tgtgtacctg gcgacaaaca gccttcaggg gccgtggtgg
     1381 atactcagct ggccggagcg ggtgccgggg gcggacgagg tgctgccgcc ggagccgccc
     1441 gcataccggg tgctgacggg cgtggtggac ggcttcgggc ggacgctggc ctttcaccgg
     1501 gcggcggagg gtgatgtggc gggcgcggtg acgggggtga cggacggcgc gggccgccgt
     1561 tttcacctgg tgctgaccac acaggcacag cgggcggaag tattccgtaa acagcgcgcc
     1621 acgtctttat cttcccctgc cggtccccgt tctgcttcct cttcttcctt ttcttcagct
     1681 ttccccgaca cgctgcccgc cggtacagag tacggtgccg ataacggtat ccggctggag
     1741 gcggtgtggc tgacacacga cccggcatac ccggatgaac agccgactgc gccgctggcg
     1801 cgctacacgt acacggccag cggagaactg cgggcggtgt atgaccgcag cgggacgcag
     1861 gtgcgcgggt ttgcttatga tgcggagcac gccgggcgga tggtggcgca ccattatgcg
     1921 gggaggccgg agagccgcta ccggtatgat gataccggcc gggtgacgga gcaggtcaac
     1981 ccggaggggc tggactaccg ctttgagtac gggcaggacc gtgtgaccat cacggacagc
     2041 ctgaaccggc gggaggtgct gtacacggag ggcgagggcg gcctgaagcg ggtggtgaag
     2101 aaggagcatg cggacgggag cattacccgc agcgagtatg atgaggcggg gaggctgaag
     2161 gcacagacgg atgcggcggg gcggcggacg gagtacagcc tgcatatggc gtcgggtgcg
     2221 gtgacggcgg tgacgtaccc ggacgggctt cgcagcagcc gggagtatga tgagaaggga
     2281 aggctggcgg cggagacctc acgcagcggt gagacgacga gttacagcta tgatgacccg
     2341 gcgagtgagc tgccgacagg gatagaggat gcgacgggca gtacaaaaca gatggcatgg
     2401 agccgttacg gtcagctgct ggccttcacg gactgctcgg ggtacacaac gcggtatgaa
     2461 tacgaccggt acggtcagag gacggcggtt caccaggaag aaggcctcag tatttacagt
     2521 ctctatgaca gccgcggcag gctgacaggg cagagggatg cgcaggggcg ggagacgcgg
     2581 ctggagtaca gtgccgcagg cgacctcacc gttatcattg ccccggacgg cagccgcagt
     2641 gagacacagt atgatgcgtg gggaaaggcc atcagcacca cgcagggcgg gctgacgcgc
     2701 agcatggagt atgacgctgc cgggcgcatc accgtgctga ccaacgagaa cggcagccag
     2761 tccacgttcc ggtatgaccc ggtggacagg ctgactgaac agcgcggttt tgacggccgg
     2821 acgcaacgtt accactatga cctgaccgga aaactcacgc agagtgaaga cgaggggctt
     2881 atcaccctct ggcactacga tgcgtcggac cgcatcacgc accggacggt gaacagcgac
     2941 ccggcagagc agtggcagta tgatgagcac gggtggttaa ccaccctcag ccataccagt
     3001 gaaggccacc gcgtgtcggt ccactacggc tatgacgata aaggccgcct gacggatgaa
     3061 cggcagacgg tggagaaccc ggagacgggg gagatgctgt gggagcatga gacggggcat
     3121 gcgtacagcg aacaggggct ggcgacccgt caggagccgg acggtctgcc gccggtggag
     3181 tggctgacgt atggcagcgg ttatcttgcg gggatgaagc tgggcggaac accgctggtc
     3241 gagtacacgc gggaccggct gcaccgtgag acggtgcgca gcttcggcag catggcaggc
     3301 agtaatgcag catatgaact gaccagcaca tacaccctca caggccagct ccagagccag
     3361 cacctgaacc tcccgcagct tgaccgtgac tacgactgga acgacaacgg acagctaatc
     3421 cgcatcagcg gcccgcagga gagccgggag taccgttaca gtgacacggg aaggctgacg
     3481 ggcgtccaca ccacggcagc gaacctggat atcgatatcc cgtatgcgac ggacccggca
     3541 ggaaaccggc tgccggaccc ggaactgcac ccggacagca cgctcacggc gtggccggat
     3601 aaccgcatcg cggaagatgc gcactatgtc taccgccacg atgaatacgg caggctggcg
     3661 gagaagacgg accgcatccc ggaaggggtg atacggatgc acgacgagcg gacccaccac
     3721 taccactacg acagccagca ccgtctggtg ttctacacgc ggatacagca cggtgagccg
     3781 caggtggaga gccgctacct ctatgacccg ctgggccgcc ggacgggaaa acgggtgtgg
     3841 cggcgggagc gtgacctgac ggggtggatg tcgctgtcgc gtaaaccgga ggtgacatgg
     3901 tacgggtggg acggtgacag gctgacgacg atacagaccg gcaccacgcg tatccagacg
     3961 gtatacgagc cggggagctt cacaccgctc atcagaatcg aaacagagaa tggtgaacag
     4021 gcgaaggcgc ggcaccgtag cctggcggag gtgttgcagg aggacacggg tgtgacgcta
     4081 ccggcggagc tgtcggtgat gctggggagg ctggagcggg aactgcggca gggcagcgtg
     4141 agtgaagaaa gccagcagtg gcttgcgcag tgcgggctga cggcggaaca gatggccgcg
     4201 cagctggagg cggaatatat cccggagcgg aaacttcatc tttaccactg cgaccaccgg
     4261 ggactgccgc aggcgctcat cagcccggaa ggcgaaacgg cgtggcaggg ggagtatgac
     4321 gagtggggaa acctgctggg cgaaaccagc gcgcagcacc tgcaacagcc gtaccgtctg
     4381 ccgggacagc agtatgatga ggagtcgggg ctgtattaca accgcaaccg gtactatgac
     4441 ccgctacagg ggaggtatat cacccaggat ccaatagata taaaaggagg atggaattta
     4501 tattcttatg cgcttaatcc ggtaagttgg attgacccat taggattgac gcagtgcgat
     4561 tccgaagggt gtaataatga tatattattt acaggaggaa gtggtcccga taataaaata
     4621 cttaatgaat taggtccgag agatggcatt gacggtttgg gttcacaaaa catgaaaatg
     4681 tatagtgggc tattaggggg cgatatttta aaaccgggga ttctgggcgg tttgacgctt
     4741 ggtagtgtga cacaacgacc atcgcgaacg gctgaggagg aggtgcaagc tcaaatagaa
     4801 tatttagcat ataagaagcg ttgtgagcaa aaacctgata gtaatttaag tcgttgtgct
     4861 gccgcgatct ttcaaaagag cagaaaagaa gattgtttaa gaatgagaca agaatgggat
     4921 gataaatggt ggcctggcaa acatgcggat gaaataaaaa atgtgaaagc atcgttgaaa
     4981 aacacactta ttgatgttaa aaaaagatgc atgccaacta gtggcattcc gagataggta
     5041 tccttaa
//
LOCUS       DAZGCQ010000220         5012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000220 DAZGCQ010000000
VERSION     DAZGCQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.220"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..555)
                     /locus_tag="ACHK07_005058"
     CDS             complement(<1..555)
                     /locus_tag="ACHK07_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8105251.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYN"
     gene            complement(555..884)
                     /locus_tag="ACHK07_005059"
     CDS             complement(555..884)
                     /locus_tag="ACHK07_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105252.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKRY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(881..3442)
                     /locus_tag="ACHK07_005060"
     CDS             complement(881..3442)
                     /locus_tag="ACHK07_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8105253.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRMLPMQFTDVATQLAGGQNPFLIMLQQGGQVKD
                     SFGGMIPMFRGLAGAISLPMAGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTAERMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDSINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTLMASAKKDYEST
                     QKKPKSSGVRVSAGERQEDRAHAALLALETELRTLEKHSGANEKISQQRRDLWKAESQ
                     YAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAARFE
                     EQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADEDM
                     LRGDWLAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTR
                     SVLSMMTEILLKQAMVGMVGRIGSAIAGAFGGGASASSGTAIEAAAANFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3423..3836)
                     /locus_tag="ACHK07_005061"
     CDS             complement(3423..3836)
                     /locus_tag="ACHK07_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8105254.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEEDMLMQKAAGLAGGVRFGEEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(3863..4294)
                     /locus_tag="ACHK07_005062"
     CDS             complement(3863..4294)
                     /locus_tag="ACHK07_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8105255.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMSEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNVLTDDDFSVGKSSTAS"
     gene            complement(4311..>5012)
                     /locus_tag="ACHK07_005063"
     CDS             complement(4311..>5012)
                     /locus_tag="ACHK07_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105256.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAEPYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          985 a         1704 c         1195 g         1128 t
ORIGIN      
        1 gttatacccg cactcatcgc cccggtaatc ccacatacag gtgttcgcca gcatgatgcg
       61 accgggaaac agcgcaccgt ccgtctccgt cggtgtcgcc agcacaaacg aggccgtcat
      121 ggccgtcagc gctgataact gctccaccac ccaccggtcc gtcagctcct gctccgggtc
      181 tgcctccgga ttccccgcca caaagttcac cgcatcaaga aaacgcgcat acacccggcg
      241 gcggaccacc gtggcaccca ccaggctctg caaatcctcc gccatcccgg tgacaaggcc
      301 gaaaagattg gacaccgtca gcgacgggcg ggcactgctg ccctttccgt tcatctcaaa
      361 gccgctgccc tcaatcgggt acgcctcata cttccgcccc tgccaggtca ccggctcccc
      421 tttttcattc agctcattgc agaaaaaata ccgctcaccg ccctgcaccg tcaggtcgat
      481 ttcccagagc accacccgcg gtgactgctc tgacttaacc gactcgttca ggctttcttc
      541 gtgaatatcc tgcatcagtt caccacctgc ttaaactccg cgctgaactc aacgcgcaac
      601 atcccgaccc gcgcagacca cccggcgcag gtcaccttta tctgccggta tgcatagggt
      661 ggcttccaca aaaatgcctt ccagcccccg tgctctgcca gaaacgcttc cagatgccgg
      721 gcctcctccc gggtcacgga aagcatcacc ctgtacctct tcaggtctgc attcagccct
      781 gccgccatac gctgtgagta cccgtcacca aaacgcactt cacgcaccga cggctgcgag
      841 ttcacctcca tatccggctt cactttccag cgaaaggttt tcatcgcccg ctccctgata
      901 acataccgcc atcacgcaac tgcagccgga gttcatcctg cgccccctta cgggccatgt
      961 catacaccgc cttcatcagc tgcggccccg cctgtccgtt tataccgtcg ttctgaatca
     1021 ccacgtgatt gttctgatta aaattaatac cttccgcccg ccgcatctgc gccggacttc
     1081 cggcaccacc cacataaccc ccttccgcat agccgcgcat caggcggtaa agattcccca
     1141 cacctatccg gctggttgcc tccttcgtga agacaaactc cccgcggtga actatccccg
     1201 caggctcata tttgccgccc gtccccgtaa atcctccggt cgcgaaatgg aagttcgccg
     1261 ccgcagcctc aatggccgtc cccgaggaag cagatgcacc accaccgaaa gcaccagcaa
     1321 tggcgctgcc gatacgcccg accatgccca ccatggcctg tttaagcagg atttctgtca
     1381 tcatggacag caccgaacgg gtgaatcccc gccagttctg ttcactgccg gtcagcatcg
     1441 ccgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc cacactttta acctgcgaaa
     1501 aactgtccgt cgcactttcc gcccactccc cccagccgga cttcagcccg gccagccagt
     1561 caccgcgcag catgtcttca tccgcccatg tctgtttcag tgccccggtg acccgggcca
     1621 gcgcctgcgg attatcgcca tacacgtcac gaagacgctg cgcttcagac tcccgctgcg
     1681 cctgacggtc agtgagacca cgggcttttg cgctgatggc ggcctgcttc gcgctctgct
     1741 gctcttcaaa ccgtgccgcc tgctgtgcca gctcattcag ccgtttctgg tattcaacct
     1801 tgtcgcccag ctcagccagc tgacgtttgt actccagcgt ctctttctca tgggccagca
     1861 gggatttttc ctgcccggat aactgtcgtt tcgtggcagc ctctttcagg accgcatact
     1921 gactttccgc tttccataaa tcacggcgct gctggctgat tttctcattc gcaccgctgt
     1981 gcttctccag cgtccggagc tcagtttcaa gcgccagcag ggctgcatgc gcccggtctt
     2041 cctgacgctc cccggctgac actctgacac ctgacgactt cggttttttc tgcgttgatt
     2101 cataatcctt tttcgccgac gccatcagcg tgttgtaatc cgcctgcagg attttcccgt
     2161 ctctcagagc cttgttcagt tctgcctgac gggcggtata tttctccagc ggtgtctgca
     2221 ggcgttcgta agccttctgc gcctcttcgg tatatttcag ccgtgacgct tcggtatcgc
     2281 tctgctgctg cgcatttttg tcctgttgag tctgctgctc agccttcttt cgggcggctt
     2341 caagcgcaag acgggccttt tcacgatcat cccagtaacg cgcccgcgct tcatcgttaa
     2401 caaaataatc atccttgcgg agattccaga tgtcgtctgc tttcttaaac gcagtctctg
     2461 ccttaatcag catctcctgc gcggtatcag gacgaccaat atccagaatg gcatcccaca
     2521 tcgatttgaa cgccttccct gttttatccg cccaggtttc cagcgtcccc atgttctctt
     2581 tcaggcggcg ggtctggtca tcaaagcctt tcgtggcagc ctcgttcgcc gcctgcaatg
     2641 ccccggcctc gtctccggaa cgctgcagct gcgcaacata cgcaatctgc tctgccgtca
     2701 cgttacggaa ctggcgcgcc atcgccatca gtcccgacgt cgggtcggtg gtcagttttc
     2761 cgaaagcctc tgcaaccttg tccacttcca caccggaggc agacgcaaaa cgcgcgacac
     2821 tctggttgat ggaatcaaac tgttcaccac cacgcacacc ggcattcacc agggctgcca
     2881 gtgactcact cgcctggtta aacgtcagcc ctgctgcctg tccggctctg gagagcgtaa
     2941 gcatgcgctc tgccgtcagt cctgactgat taccggaaag aaccagggtt ttattaaacg
     3001 ctgaaagcgt ggaatccccc tggtaccagg cgtacgccag cgcaccggtc gccaccgcca
     3061 gcgaggtgac accggccatt ggcaggctga tcgcaccagc aagcccccgg aacatgggga
     3121 tcatcccgcc gaaggagtcc ttcacctgac cgccctgctg cagcatgata aggaagggat
     3181 tctgaccacc ggcaagctgc gtggccacgt cggtgaactg catgggtagc atacgcatgg
     3241 cggctttata ctgcccgact gatatcccgg ctttttgtgc agccagcgcc tggcggctca
     3301 gcccctgctc aacagcactg gcggtttttc tggcgtcagt ctccagtcct gaaaaatgac
     3361 gccttacgcg ggtcatctgc tcatcgaaac gaaccgcatc cagactcagg tcaataacaa
     3421 gatcaccaac cggctgggac atatctcaca cctccggaaa tccccgctga agccatcatt
     3481 aatgcgacat catcctcgct gacatccacc acatccgcag aaggtgaaat atcgccccct
     3541 ccctcctcgc cgaaccggac gcctccggca agtcctgccg ctttctgcat cagcatgtct
     3601 tcctcatccg gcctctccgt ctgcgcttcc tcacgccggg ggacgagcag actgaaatcc
     3661 gagggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacgt cagcccggaa
     3721 aaatgcatat ccagctgggt atcgtgaaaa taatgcgtgc ggtaaaaacg tcgccagtcg
     3781 gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct ccccatctca
     3841 cgcgccagtc tgagggcaaa attcagctcg ccgtcgaaga ctttcccaca gaaaaatcat
     3901 catcagtcag cacgttattt ttcgccactt cggtgatatc agtatccgga cgaacagctt
     3961 cgatcatccc ggacaggcac aacacaacat cttccgcccg ggcaatggca tcggcaggcc
     4021 aggtggtgag cacttcctgc tctatcttca tcacggcctc actcattgac ggtgactgcg
     4081 ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgcgccg gttctgacaa
     4141 gatcttccac actcacctgc aggttgccgc tggcttcagc ctcttctgcc cgccgtttca
     4201 ggagggcaag atgctcaata cgctgcagcg cagacagctc agaaagcatg acggatacac
     4261 cgttatattc aaattgttct gttttcagga acatgcttga tctcctcccc tcagcccgca
     4321 gcgccatccg tgacggtgat ctccgccacc gccgcaaact caccgttgcc ggtgacaaca
     4381 gggatctgcg ctttacctgc cgcaacacct ttcacggtga tcgtgttccc tttcacggta
     4441 atggtggcga aattctgatt tgccgacgtt gcgcggaatg ttttatccgt tgcgccttcc
     4501 ggctgaacgg ccacagtcag ggtggtgctc tgaccttttg ccacattccc cgttagtggc
     4561 gtcacggtaa taccggtgac cggtgtgatt tctccccgat cctccgccag cgaaggacga
     4621 ccgatattgg tgatttttac cgtacgggta atcacttctt tggcggtcac cgctttacca
     4681 atggcgctca cccagccacg gaacacatcc accgtaccgt tcgggaagcg gattttgtag
     4741 gcccgggtct cactgctgtc aaaccaggca atcaaatcac gctgcccttt ctcgccgggc
     4801 ttccaggcca gcgtaaaact ggtgtcaccg gcggatttct gcccctgccc ggtggatacc
     4861 cagtcggcat cctcatcatc cagatagtta tcgtcgtagg gttccgccgt catctcgccg
     4921 ggggtcagat cctttatttt tgccagacgc gtccactcat catctgacag cgggtttgca
     4981 taagcatcgc ccttaccggt gtaaacccag ag
//
LOCUS       DAZGCQ010000221         4969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000221 DAZGCQ010000000
VERSION     DAZGCQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.223"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            430..588
                     /locus_tag="ACHK07_005064"
                     /pseudo
     CDS             430..588
                     /locus_tag="ACHK07_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            812..931
                     /locus_tag="ACHK07_005065"
     CDS             812..931
                     /locus_tag="ACHK07_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8105257.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            996..>4969
                     /gene="ypjA"
                     /locus_tag="ACHK07_005066"
     CDS             996..>4969
                     /gene="ypjA"
                     /locus_tag="ACHK07_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO8105258.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTSDVIGVVNDETV
                     DGTQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTVKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDGTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGSVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSH
                     IGFDR"
BASE COUNT         1446 a         1081 c         1295 g         1147 t
ORIGIN      
        1 taactcccct gaggatgctc ccattgagaa tagtcctatt gtgaatagtc cccttgtatg
       61 aagttctaaa agtagttttc aatgataaat aaatggctat ttttacgaag agataataac
      121 gtagattgaa acaatatgta ttattgattg agtaaatggg attattcttc acactacatc
      181 cattttagtg tcactaccaa agaaaaaggg gctacgtttt catgtaaccc cttgatttat
      241 ttggtgggct agcagtgact gaattggtgc tgtaattggt tgaaatattt catctaatgt
      301 ctggttcaac ttccttagct gcctgaaaat tttttgagct tacccagcaa taacagcgtt
      361 acggtgcacc acgcctgact gaagaactgc gtgctcaggg ttaccacttc aacgtaaaac
      421 cgtggcagga ggtccggact gaaaaaactg ctcaggacat catcggcagg gaacgcactg
      481 ctagtctgta aactgaaccg actggggcgc agtatgtggc atcttgtcgt gctgttggag
      541 gagctgtgcg aacgtggtat taactttcgt gctctggccc aatctatatt tgcccaacaa
      601 tggggggacg aatgctgtaa aagtaaaaca atctgcgatc tcaaagttat tgtgtgattt
      661 ttatgtgagc agaagatatt catcagaaac gattatgtaa atcattttat tttgccaacg
      721 gcctcattgt cgaaagaaaa gaatacgccc gtgtttcctg aatagattga cttttttatc
      781 caaccacact tcagcgcact gcgtttaaaa aatgcctcat tcttatgcgg aatatcatca
      841 tttcatcatg atgtctttga tgagcggtga acacaataca cttgcgctgt ctcttcagga
      901 tgaatcccct ggtctggtgc ctgtgggctg atgttgcagc aaagctaagg tcgcttaaac
      961 gctactcagt attcactttt cagaggatga aatttatgaa caggaccagt ccccattatt
     1021 gtcgccgctc agtactttcc ttattgatat ctgccttgat atatgccccg cccgggatgg
     1081 cggccttcac ttctgatgtt attggtgtgg taaacgatga gactgtagat ggcacccaaa
     1141 gagtagatga acgaggtaca acaaataaca ctcatattat caaccatggc cagcagaatg
     1201 ttcatggcgg ggtatctaat ggaagtctta ttgaatctgg tggatatcaa gatgtaggaa
     1261 gtcataacaa ttttgtgggg caggctaata atacaaccat taacgggggc agacagtcaa
     1321 ttcatgacgg gggtatttcc acaggtacga taatcgagag tggcaatcag gacgtttata
     1381 aagggggtat cagcaatgga acgacagtta agggcggtgc ttcacgcgta gagggaggga
     1441 gtgcgaatgg aacactcatt gatggtggta gccagatagt aaaagttcaa gggcatgctg
     1501 atggtacaac gataaataag tctggctctc aggacgtagt acaaggaagt ctggcaacga
     1561 acacaaccat aaatggtggt cgacagtatg ttgaacagag cacagtagaa acaaccacca
     1621 tcaaaaatgg cggtgagcaa agagtatatg agagccgtgc gctggacacg acgattgaag
     1681 gcggaactca gtctctgaat agtaagtcaa cggcaaaaaa tacgcagatc tattctggtg
     1741 gcacgcaaat tgttgataac accagcacct cggatgttat tgaagtttat tccggtggcg
     1801 tgcttgatgt tagtggtggt acggcaacaa atgttaccca gcacgatggt gcaattttaa
     1861 aaactaacac taacggtacg acggtgagcg gtacgaatag tgaaggtgca ttctccatcc
     1921 acaatcacgt ggcagacaat gtgttgctgg aaaacggtgg tcatttagac ataaacgcat
     1981 atggttcggc aaacaagacg attattaaag ataaaggaac aatgtcagtt ttaaccaatg
     2041 ctaaagctga tgcgacccga atagataatg gcggggttat ggatgttgca ggaaacgcga
     2101 caaataccat aattaatggt ggcacacaga atattaataa ttatggcata gccacaggca
     2161 ccaatatcaa cagcggaacg caaaatatca aaagcggcgg gaaagctgac acaacaatta
     2221 tatcctccgg gagccggcag gttgttgaga aagatggtac ggcaattggc agcaatatta
     2281 gcgccggagg ctcgctgatt gtctataccg gcggtattgc acatggggtt aaccaggaga
     2341 cgggcagtgc tttagttgcc aacacgggtg cagggactga tatcgaagga tacaacaagc
     2401 tctctcactt cactattacc ggaggggagg ctaattatgt tgtgctggaa aataccggcg
     2461 aactgacggt agtggctaaa acctcggcga aaaatactac cattgatgct ggcggtaagc
     2521 tgattgtcca gaaggaggct aaaacagatg gcaccagact taataatggc ggcgttctgg
     2581 aggttcagga cggtggtgag gctaagcatg ttgagcaaca atccggcggc gcattaattg
     2641 cttccacgac ctccggaaca cttatcgaag gaaccaacag ttatggtgat gctttctaca
     2701 tcaggaattc agaagctaaa aatgtagtgc tggaaaacgc tggctcatta acagtcgtca
     2761 ctggttcccg ggcagttgac acgattatta atgccaacgg caaaatggat gtttatggaa
     2821 aagatgttgg ctctgtactc aatagtgctg gcacccaaac aatatatgcc agtgccactt
     2881 ctgataaagc aaatatcaaa ggtggcaagc aaacggtata tggtttagcc actgaagcaa
     2941 atatcgaaag tggtgaacaa attgttgatg gtgggtcaac agagaaaaca cacatcaatg
     3001 gtggcacgca aaccgttcag aattatggta aggcgatcaa taccgatatc gtctctggcc
     3061 tacaacaaat tatggcaaac gggacagcgg aaggttccat tattaatggc ggttcacagg
     3121 tagttaatga gggcggtctg gctgaaaact cggtgcttaa tgacggcggc acactcgatg
     3181 tgcgggagaa aggcagcgca acggggatac agcagagtag ccagggcgcg ttggttgcaa
     3241 ccaccagggc gacgcgggtc acaggaacac gcgcggatgg cgtcgcgttc agcatcgagc
     3301 agggtgcggc gaacaatatc ctgctggcaa atggcggcgt gttaaccgtg gagtcagaca
     3361 cctcttctga caaaacacag gtcaatacgg gcggacggga gatcgtcaaa acaaaagcca
     3421 ctgcgacagg cacgacgctc accggcggtg aacaaattgt cgagggtgtg gcgaatgaga
     3481 caacaattaa cgacggcgga atacaaacag tttcagctaa cggagaggca ataaaaacaa
     3541 agatcaatga aggcggtacg ctgacagtca acgataatgg caaagcgaca gatatcgtcc
     3601 agaacagcgg tgccgctctc cagacgagca cggctaacgg tattgaaatc agcggtactc
     3661 accagtacgg cactttttcc atttccggca atttagcgac caatatgttg ctggaaaatg
     3721 gcggtaattt attggtatta gcaggtaccg aagctcgcga ctccacggtt ggcaagggtg
     3781 gggcaatgca aaacctgggt caggactccg ccacaaaggt taactctggc gggcaatata
     3841 cccttgggcg gtcaaaagat gagtttcagg ctctggcccg ggcagaagat ctccaggtcg
     3901 ctggcgggac ggcaatcgtc tacgcaggta cgctggcgga tgcatcggtc agtggcgcga
     3961 caggaagcct gtcgttaatg acgccacggg ataatgttac gccagttaaa ctcgaagggg
     4021 cggtccggat taccgatagc gcgacattaa ctctgggaaa tggcgtcgat acaacgcttg
     4081 ccgacctgac ggctgccagc cggggcagtg tctggcttaa cagcaataat tcctgtgcag
     4141 gtaccagcaa ctgcgaatat agagtaaaca gtttgctact caacgacggt gatgtttatt
     4201 tatcagcaca aacagcagcg cctgccacaa ctaacggtat ctacaatacg ctgacaacca
     4261 atgaactttc cggtagcggt aatttctacc tgcataccaa cgttgcaggc tcccggggcg
     4321 atcaactggt cgtcaacaac aacgccactg gtaattttaa aatctttgtt caggataccg
     4381 gcgtcagccc acagtctgac gacgcgatga cgctggtgaa aacaggggga ggggatgctt
     4441 cgttttcgct gggcaatact ggcggtttcg ttgatcttgg gacctatgag tatgtcctga
     4501 aaagcgatgg caacagcaac tggaacctga ccaatgatgt caaacccaac ccggatccca
     4561 acccaaatcc caacccaaat ccgaagccgg atccaaaacc agacccaaaa ccggatccga
     4621 aaccagaccc gactcccgat ccaacgccga cacccgttcc ggagaaacgc atcacgcctt
     4681 ctacggcagc cgtactcaat atggctgcaa cattaccgtt ggtatttgat gctgagctaa
     4741 acagtattcg cgagcggttg aacataatga aagcgagtcc acacaacaat aatgtctggg
     4801 gggcgacgta taacacccgt aataatgtca cgaccgatgc gggggccggg tttgagcaga
     4861 cgctgaccgg aatgacagtg gggatcgaca gccgtaatga tattcctgag gggattgcga
     4921 cgctgggcgc ttttatgggt tattcccatt cacatatcgg ttttgatcg
//
LOCUS       DAZGCQ010000222         4949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000222 DAZGCQ010000000
VERSION     DAZGCQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.224"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..1551
                     /locus_tag="ACHK07_005067"
     CDS             127..1551
                     /locus_tag="ACHK07_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO8105259.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIISSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
     gene            complement(1794..2104)
                     /locus_tag="ACHK07_005068"
                     /pseudo
     CDS             complement(1794..2104)
                     /locus_tag="ACHK07_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611576.1,RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2470..3279)
                     /gene="iolB"
                     /locus_tag="ACHK07_005069"
     CDS             complement(2470..3279)
                     /gene="iolB"
                     /locus_tag="ACHK07_005069"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFO8105260.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(3304..4809)
                     /gene="iolA"
                     /locus_tag="ACHK07_005070"
     CDS             complement(3304..4809)
                     /gene="iolA"
                     /locus_tag="ACHK07_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFO8105261.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT         1263 a         1069 c         1185 g         1432 t
ORIGIN      
        1 caaactattt ttcacagtta tgaaattttt atattttgtg atctttgttg tggaatattt
       61 gagctgattt gtttaagtat ggaataaatg ttcgtatgtc atttcctcaa caggcaggga
      121 gggggtatgt ttcagagaag caagtatgat tcagcctatg tgtacgcatt gtgctgcgtt
      181 gctgcactgg ctggattgat gtttggctat tcgacggcag tgataacggg ggtgatactg
      241 cctttacagc aatattacca gctcacttca actgaaactg ggtgggcagt atcgagcatt
      301 gtgattggtt gtatcattgg tgcgctggtc ggggggaaaa ttgccgataa gttggggcgt
      361 aagcctgcgc ttctgattat tgcagttatt tttatcatct cgtcactgtg gggggcgatg
      421 agtgaatctt ttatggtttt ctcgatttcg cgcatcgtca gtggttttgc tgttgggatg
      481 gcggggactg catctaccat gtatatgtct gaactggcac cagctgacat tcgtggtaaa
      541 gctctgggta tttacaatat atcagtagtt tctggtcagg tgattgtttt tattgttaat
      601 tatgtgattg ctaaggggat gccctctgat gtgctggtcg aagaaggctg gaagagcatg
      661 ttattcgctc aggtgatacc ttctgttaca atgttagtga taactttatt tttgcctgaa
      721 tcgccggcat ggtgtgcccg taataacaag ggggaaaaac gttctctgaa agtgcttgcc
      781 agaatctacc ggggggttag cgctaccgat gtagccgcta tttttgatag tatgagggaa
      841 acggaggccc gggagaatgc tgcaggcagg gaattcgtta tcctgaaaag ctctcctgtg
      901 ttgcgctata ttctgctggt tggctgttgt atcgctgttt tacaacagtt tactggtgtc
      961 aatgtgatga actactacgc acctctggtg ctacaaaaca gcagtacaga ggaagtgatg
     1021 ttccagacga tttttattgc tgtttgtaat gtgatcggta gttttatcgg gatgattttg
     1081 ttcgatcgct atggacgaat accaatcatg aagattggca ctatcgggtc aattattggt
     1141 ttgttgatcg catcatatgg tttgtatacc catgatactg gctatatcac catttttggg
     1201 attttatttt ttatgctgtt gtttgctgtc agctggagcg taggagcatg ggtactgatt
     1261 tctgaggtgt ttcccgaaaa gattaaagga ttcggcatgg ggctggcggt tagtttgatg
     1321 tggattgcga attttctaat atcactgttg tttccggtaa taaatgataa tacctggctg
     1381 caggaaacct ttggcggagc gttttctatg tggatttttg ttgtctttaa tctggtttgt
     1441 tatgttttta tttctcgcta tgttcctgaa acaaagggga tcccgctaac acaaattgag
     1501 cagcttgccg gaaataaact gcgtgaaatt cagggtaaag ctatgatgta atagtctgat
     1561 tgtgtcacaa tcagcagtaa tgcccggtaa tgttgagcat cttaccggga ttttccttgt
     1621 gttaatatag gtaacaaaag gagataataa atatccagaa ggttttttat aaccaactat
     1681 aaatatattt ctgattgcat ttgatacacg tattttattt ggtatttgtg ttaaatttta
     1741 ttaatttcta ttcataaaca ggttactctg atatgggagc aacaaccatt cggctatatt
     1801 atctttttca tgttggtgct tttattctgt gaggagtgct ggcatggcgg acacaaccat
     1861 ttgttctttt tagctgccat ttgataatat tttcctgaag ctttccgggg atatattccc
     1921 cagacgggag tggcggcgct gacggttgta gaatatctca atgtataccc agtattactg
     1981 agatgacttc attccggcta ctaaaatggt agtggttcag actttcattc ttcagagttc
     2041 cccaaaagct ttccataggg gttttgtcgt aacagttccc tttacgagac attgatgttt
     2101 tcagagctaa ctgcgataga ctccttcaac ttcaacttca acttcaactt caactgagaa
     2161 acggatgatt tcacgataga ctgtccaacg tattgagccc gttaggaagc tagagtaaca
     2221 agccgccgtg tacggcattt ctctcctaat tcggcagaac caaaaatatt gtcagctcag
     2281 gagtaagaat cagaaaaaac cataacagaa caccagaaac aggatattat cagacagaga
     2341 aaaaattcaa aaaaatccac cgattctgtt gcctgtggat ttgtgtataa gaggcagaac
     2401 cctgttgggg actgtgcaca aaaaagccag ctgcagagga caactggcaa aggaatattt
     2461 aacgtattgt taacgcggat actctgacga attaatccat gcgtgatcag cttcccatgt
     2521 gaattgccat ttgcgtaatg gcccggccat cacattcaga taatagttat cgtaacctgc
     2581 gatagtggcc accggatgat agcctttcgg gacttttacc acatcgcggt tatatactgc
     2641 catacattcg tccagactgc gatcggcggt gtagactcgc tgtaagctga atccctgggg
     2701 tggatcgaaa cggtggtagt aggtttcttc cagataggtt tcctgccctt caactgctgt
     2761 gtcatgctta tgggcaggcc aggaacttgt atcgccttcc cttgtatata cttccactac
     2821 cagcagactg tcagcaggtt gagtgtctgg gaggatatta tgaaccaagc gctgattgcg
     2881 cccttttccc ctatgttcca ccccaatatc ttgtggtgag atcagtctta ccggaagttc
     2941 accgaatcca ggagcactgc agacagccag ttccagatca gtttcggcag tgacttttgc
     3001 ttctgtatgg tgcgggaggt aaacagaata agccggaatt cgctcaaaag gactcatgcg
     3061 ctggccaatc cgataaaaaa agtggtcagc ggcctttacg ctggcaagtc cggcaaccag
     3121 aaccaggcaa cgttcgcgat catcggatgg caaggttaaa gattcgccgg ctttcaacag
     3181 ccagacatca aaaccgacat actcccagcc tgcgttttgc ggagaaatat gttgaatatt
     3241 tccagccctg gtggtgcagg tactcagcaa gttggtcata atcttctcct tcctgaacgt
     3301 ttattaacct agcgtaggca tactgaactc ggatgcgatc tgctgaccgt tcggccagcg
     3361 tgcggtaacg gttttcatcc gggtataaaa acgcacccca tccggtccgt ggacattcag
     3421 cgccccaaac accgagcgtt tccagccgcc gaaagagtga aacgccattg gaaccggtac
     3481 cggaacattg ataccgacca tcccggcttc aaccgattga gcaaattcgc gggcataatg
     3541 gccgttggag gtgaagatca cgctaccatt gccaaattca tgagagttta ccgtacgaat
     3601 ggcggtggca taatctgtta cgcgtacgat gccgagcacg ggaccaaaaa tttcctcacg
     3661 ccagatagtc atttccggcg tgacgttatc aaacaaggta ccgccgacat aaaatccttc
     3721 aggatagccc ttcacgcaat aatttcgccc atccatcacc agtgttgctc cttcgctaac
     3781 gcctttgtct atatatgcaa ggactttttg ttgatgcata cgtgaaacaa ctggccccat
     3841 ttcattttcg tctttttctc gtaactcacc ggggccaata cgcagcgctg caataagtgg
     3901 ttttaggcgt tcaataagac ggttggctgt ttcatcaccg acagcgacca ccacaggtaa
     3961 tgccatacaa cgttcgcctg cggaaccaaa agccccaccc ataatagcgt taacggtagc
     4021 atcaaggtca gcatccggca tgacaatagc gtggtttttt gcggcaccga aagcttggac
     4081 tcgcttaccg tgtgcactgg cggtggtgta gatatgctca gcgacggaag aagatccgac
     4141 aaaactaacg gcctgaatgc gtggatcact ggtcaacagg ctggctgcgt cattgccaca
     4201 gtgaactaca ttgaatacac catccggtag tccggcctcc tgtagtagtt gcgccatacg
     4261 taacgatgcg gagggcacca gcgctggagg cttcaggacg aaggtattgc cgcaggcgag
     4321 agcgataggg aacatccaca ttggcaccat tgcagggaag ttaaagggtg tgatgccagc
     4381 tacaacacca agtggttgca ttaatgaaaa gctgtcaacg cctgtgccaa cattaaaaga
     4441 gaactctcct ttaatgagat gcggaatgcc gcaggcgaat tcaacaactt cgattccgcg
     4501 agtcagttca ccaagcgcgt cggaccatac tttgccatgt tcactgacga tcagagccgc
     4561 cagttcatct cggtgttttt ccagcaacac tttaaattca aaaaggattc ttgcacggcg
     4621 caacggtgcc attttactcc atgcaggaaa agcttcatga gcactctgaa tcgcctccag
     4681 catatcttgc cgggtgctct gggtaaccag gcgaatcact tctccggttg cagggttagt
     4741 caccggtagt gtttcaccac tgttgctcag tctcgtttta ccctgaataa aatttccaac
     4801 tgtttccatc actgtttacc ttcttgaatc aatgttaacg acgttccgac tgagtgttca
     4861 caggcaaagc cttatcatct acagtctaaa atcacacagt gtttaatgta tagcttcgga
     4921 ttataaattt caaatttaaa tttatgaaa
//
LOCUS       DAZGCQ010000223         4938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000223 DAZGCQ010000000
VERSION     DAZGCQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.225"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..436)
                     /locus_tag="ACHK07_005071"
     CDS             complement(<1..436)
                     /locus_tag="ACHK07_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFO8105262.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSQSRLQRLS"
     gene            complement(598..1089)
                     /gene="hcp"
                     /locus_tag="ACHK07_005072"
     CDS             complement(598..1089)
                     /gene="hcp"
                     /locus_tag="ACHK07_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO8105263.1"
                     /translation="MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NMKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(1095..2771)
                     /locus_tag="ACHK07_005073"
     CDS             complement(1095..2771)
                     /locus_tag="ACHK07_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8105264.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMQEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQSPRQVGQLSVMYRCLPDCHHDEAVLRFTLKTLRQQ
                     CKQIKSLTGFTLPVVLSAEFSGPETPWIIVRGDKPVVCPVNHAPQAFTDWLQVEDNIL
                     ALPVISEAFSFIRNTLADELEKADRLMPPVRAFSVVMRLGAVLSGTPSVWSDRLCSRT
                     CLQFSRKPEQTVPAGMFPDAVLSLLAPFASTVQGGQCTRRLILLIWLCVLTALGISAL
                     NNRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYY
                     PGQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLALFDAGKWTLKPGATKWLVNAL
                     VDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYG
                     ESRPVAPNDSAEGRARNRRVEISLVPQADACRLPDASPAPEEGASENITE"
     gene            complement(2771..3460)
                     /gene="tssL"
                     /locus_tag="ACHK07_005074"
     CDS             complement(2771..3460)
                     /gene="tssL"
                     /locus_tag="ACHK07_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO8105265.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            complement(3457..4806)
                     /gene="tssK"
                     /locus_tag="ACHK07_005075"
     CDS             complement(3457..4806)
                     /gene="tssK"
                     /locus_tag="ACHK07_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8105266.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQEGVSADRPVRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHE
                     NNDSWKVCPVARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRL
                     MAMRRESNERLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAG
                     SMLTFSLDHDPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTW
                     KASLHDIRLREEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLI
                     PVSRVPAALPVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
     gene            complement(4822..>4938)
                     /locus_tag="ACHK07_005076"
     CDS             complement(4822..>4938)
                     /locus_tag="ACHK07_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105267.1"
                     /translation="IEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT         1179 a         1470 c         1279 g         1010 t
ORIGIN      
        1 gggacagacg ctgcaaccgg ctctgactca gtgtcagcag cggccacagc ccgtcacagc
       61 gaaccaggtg cggattttcc gtcagggcac ccagcagatg cacgctgcgg atatgttctt
      121 ctccctgtaa tgaggccacc atccaggcct gtttcagcag ggcattcagg gacggcgcaa
      181 gctgcgggcg gctgcgtacg ctacggggct ggttatccag ccagccgagc agcgactgcc
      241 atattgcgtc catatcccag tcataacgcc ggcccagcac ggtaaggtct ccttcgccct
      301 gctccagcag tttcagtaac cagtgctccg gggtgatttc cgcatgggcc cgggtctggc
      361 aaagggaggc tgcgccttcc agtgcacggg cacagtaatg attaagacgg cgtaacagag
      421 ctgccggatt ttccatgtta ttcactcact gtcagaagac cttcagacac gctaccgccc
      481 ttcgccataa cccgggcgca tcaggtctgt gttcaggatt gagaattctt tcttcagcac
      541 ccgccaacag atactgaaga aaaatgacgg acaggatacc ctgcccgtcc gggagaatta
      601 cgcagtggta cgttcattcc acgcgtcaga atgaatgatg ttaccgtcct tataagtcca
      661 ggtgattttc tcgtagcgca gctccacctg ctcaaggtgg ttatgcttct cgtaggcggg
      721 attcttgatg tcgtgcatga ccgggttaac cttcaccacc ttcacgttct caagcttcat
      781 attgaaatac tccacttcct gaccggcatc attgattctg taccacttaa attcagcaga
      841 cttcagggtc tgccccgtgg tcaccgcctt gtaaaggtag gggctggagg aatcaatttc
      901 cttggtaaac aggaacggcg tgtgaatacg ggtaccggtc agcttaccgg tattgttatc
      961 cgtcggaatg taaaggcagt gctcctgagc caccacctca atgctgccct cacggtcctg
     1021 aacatccacg gaccctttga tatctgcacc gccatcatct ttcagccaca gataaactgg
     1081 aattgccata aaaatcactc cgttatgttt tctgatgcgc cctcttccgg cgcaggggaa
     1141 gcgtccggca gacgacaggc atcggcctgc ggtaccagac tgatttcaac acggcggtta
     1201 cgcgcgcggc cttccgccga gtcattcggg gccacagggc ggctttcacc gtatccctgc
     1261 acggcaaaac agctttgagg gatatcgccc gtatcccgca tccagtcgcg aaccgcctct
     1321 gcacgcttca gggacagggc ctggttgcgc tgcgggtcac cggtattgtc cgtgtggcca
     1381 ctgaccacaa tcagccagcc tgcttttgcc ttaatatcca ccagggcatt caccagccat
     1441 tttgtggcgc ccggtttcag cgtccattta ccggcatcaa acagtgccag gctatcgagt
     1501 cgtacaattt ccggcaccgg ttctggttca ggggatggtg gtggaacccg ggtatcgata
     1561 gcctgctgca gggccagcca cagacgttgt cccgggtaat agcccaggct gtaacgtaaa
     1621 ggcactccct gacgctgcca ctgctcaagc agaagcgcat cctgtttcag tgcggccagc
     1681 gattccgcct caggacggta gtgccccttc gggacagcat tccagcgctg taaatcggtg
     1741 cttacctggc ggatcagatc acgattgtta agcgcagaaa tccccagcgc agtcaggaca
     1801 cacagccata tcagcaaaat cagacgacgg gtacactggc ctccctgtac cgtcgacgca
     1861 aaaggtgcca gcagcgacag aaccgcatcc gggaacatcc ctgccggcac tgtctgctcc
     1921 ggttttcggg aaaactgcaa acaggtgcgg gaacagagcc tgtcggacca gactgacgga
     1981 gtccccgaaa ggacggcacc aagacgcatt acaacggaaa atgcccgtac aggtggcatc
     2041 agccggtcag ctttttccag ctcatcagcc agcgtattac ggataaagga aaatgcctcg
     2101 ctgatgaccg gcagtgccag aatattgtct tccacctgca gccagtcagt gaatgcctga
     2161 ggtgcatgat taaccggaca tacaactggc ttatcccccc ggacgattat ccacggtgtt
     2221 tcaggcccgg aaaattcggc actcaacacc accggcagtg tgaaacccgt cagggatttt
     2281 atctgcttac actgctggcg gagtgttttc agtgtaaaac gaagaaccgc ttcatcatga
     2341 tgacagtctg gcaggcagcg gtacatcaca gagagctgac cgacctggcg cggggactgc
     2401 gtctgtatgc tgcggacaac gtcagttaag cgactgacgt caccgacccg cagccaccat
     2461 ccccgggctg ttttacgcag cggaccgtcc tgaaacaacg cttcctgcat gtctccacat
     2521 accagtacaa cagggccccg ggtgtcggat aatgccggtt cagcatccgt cagggtaacg
     2581 tcagatgtga gcttttgctt ttcactccgg ctgtttttaa aaacactcca gcctgccaga
     2641 agaaggatca gcagagtagc cagaccgtta aaaacgttgc ctgcagggat gaaataccac
     2701 agcaaccaca gcaacgtgga aacggccacc agggcatgca gggctaccgg aatgatgaca
     2761 gatccacgca tcagccatgc aactccggta tttgtgatga caaaagagac tgaagccaga
     2821 ggtacccccc ccaccagata acgccggtaa gaatgacggc gagcactgtc cagaaccaga
     2881 ctgagcgtat cagacgccag cgatgtttct ccatccggta aacaaccagg gacagaccac
     2941 catcaggcag agaaacacgt tcagaaaggg ctttcatgac ctcatcacgc tgtgactggc
     3001 tgactgcttt caggccgtac accccctgaa aacccagtgc tgtcacccgg tggaagcagg
     3061 ttaaaacggc cgggtccggt gaaggctgac gcagaacctc tgcaatgcga tcccacagtg
     3121 cttcaccagc ccgaagagaa ccaaaaaagc gggcctgaag cggcacttta cgccaggccg
     3181 cctggccttc gtcaacgtcc ggctccccgc cttccccgct tttttcaggc ttacggctca
     3241 tcaccgtttc gtccaggagg gcacattgtg cataggtaat atgctccaca ctggcatcgt
     3301 cataaccggc acgctccagc gcttcccgca cactttcgac ctgtttaacg catttgttgt
     3361 aaagcgcagt gccatcgggc gtgagtgcac cttttttcag catggtgacg gtaagccatg
     3421 tctccatcat cagctggtcg atatcaatat cctgtctcat gagcgcaata ccgcaaacag
     3481 ttcaagttcc agggcaccaa gtaactcagg cacatagaac atacacactc cctgctccag
     3541 catttcatgt gcagcgggac tttccatatc aagtgcgaag tactggtttt ccatacgtac
     3601 aggcaacgca gccggaaccc tgctcaccgg gatcaaaggg atccccctga gtgccgcgcc
     3661 atagatctca ctgacattat caggtgaccc cgccagacag agtgccggaa atttctcagc
     3721 aacctgccag gccggagtat ccgagcgcac agacagccag aggtccgcct cttcgcgcag
     3781 gcggatatcg tgaaggctgg ccttccaggt ctgtccatcc gggcgggaca tttccagagc
     3841 aatgacccgg gatggcagac aggcctccag cagacccgtt atcaggtcga acagcggaag
     3901 aaacgcaccg gccggatcct tgtggtcata acccggtatg gcgtcaggat catgatccag
     3961 ggaaaaagtc agcatactgc ctgccagacg agcgagctcc gcccacacct gttcaggtgg
     4021 acgggaagga aaacgttcgt attccgtcag tacactggcg tgggagttca gcgcattcag
     4081 caaccagaaa agggaaacat cagcgacagc aaaatcggcg agtctttcgt tactttcacg
     4141 acgcatcgcc atcagacgct ggcgtcggga gcgtaactga cgattgagca acaccagacg
     4201 ttcgcgcaga accggacttg ccccaaataa cgccaccgga ggaataaaag acggatcctg
     4261 tcgccacgca ttctgaccat ctctgatcaa tcttgctacc ggacagactt tccatgaatc
     4321 attgttttca tgggcaaacc ggatggtgaa attaaaccgg gcaacggcta tgggctcttc
     4381 ttcagagcca aaagcatcct gcagcgtgac ccactcttcc cgatagcgaa cgggtctgtc
     4441 tgcactgaca ccctcctgct caacgttggc aacgcctggc tgcatgtggg gaagtgcaat
     4501 aacgacagtt accgcatcgc ggtcagccag ctgaccggca tctaactcac gtggcgccgg
     4561 aggcagatcg ctcctttgag catcaataag tgttccgtct tccagccaga ggcgtaactg
     4621 cgtaatctgg atcaacccgg acgacagaag actgtcatta aactcaacct gttccactcc
     4681 ccagggaaac ggggatgcca gcgcagagat accggcactg cgaaaagact cccattcagc
     4741 ctgctgctga aactgctgag gggagagcaa aacaccttcg ctccacagcg gacggaaaat
     4801 cttcatttcc cgacacctgc cttacgcttt tgccttcggc atctgcgaaa ccagggacag
     4861 actgacatcc ataccttcca cctggaaatg cggtacagcg tagagtttca cacggaaaaa
     4921 tcccgggttg tcttcgat
//
LOCUS       DAZGCQ010000224         4887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000224 DAZGCQ010000000
VERSION     DAZGCQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.226"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..117
                     /locus_tag="ACHK07_005077"
     CDS             <1..117
                     /locus_tag="ACHK07_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105268.1"
                     /translation="IEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            133..1470
                     /gene="tssK"
                     /locus_tag="ACHK07_005078"
     CDS             133..1470
                     /gene="tssK"
                     /locus_tag="ACHK07_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8105269.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESGRPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQKGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1467..2120
                     /gene="tssL"
                     /locus_tag="ACHK07_005079"
     CDS             1467..2120
                     /gene="tssL"
                     /locus_tag="ACHK07_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO8105270.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSDTMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            2123..3823
                     /locus_tag="ACHK07_005080"
     CDS             2123..3823
                     /locus_tag="ACHK07_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8105271.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSRLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQVDACRLPDITRAP
                     "
     gene            3858..4349
                     /gene="hcp"
                     /locus_tag="ACHK07_005081"
     CDS             3858..4349
                     /gene="hcp"
                     /locus_tag="ACHK07_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO8105272.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            4518..>4887
                     /locus_tag="ACHK07_005082"
     CDS             4518..>4887
                     /locus_tag="ACHK07_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFO8105273.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPH"
BASE COUNT          995 a         1253 c         1496 g         1143 t
ORIGIN      
        1 atcgaagaca acccgggatt tttccgcgtg aaactctatg ctgtaccaca tttccaggtg
       61 gaaggtatgg acgtcagtct gtcactggtt tcgcagatgc caaaggcaaa agcgtaaggc
      121 aggcgtcggg aaatgaagat ttatcgtcca ctgtgggaag acggggcttt tctgatgccc
      181 cagcagtttc agcagcaggc tgcctgggat gttcatctgg cagacagcgt tgcccgaatg
      241 gggctggcgc atccctgggg cgtggtggct gcagagtttg atgactctct gttaccgctt
      301 tcacggttga atgccacccg tctgattgtt cgtttcccgg acggaacgct tattgatacg
      361 gaacgtgcgg ataatctgcc tccggtctgt gatttatcga cggtttctga tcgttccctg
      421 gtggatattg tgctggcgct accgttactg aatgctaacg gtggcaacct tgatgatggc
      481 cgtgagagcg ggcgtccgcg tcgctggaaa tcagaaaggg tgaatgtgca ggagctggcc
      541 gggcatgagc aaagcgaagt ggcggtattg cggcataacc tgactctgcg catggctcat
      601 caggaaaatg ctgcctggct gacctgtccc gttacccgtc ttgttcgtga tgcacaggga
      661 cagtggtgca gggacccgcg ttttattccc cctctgctga cgctgtctgc cagtccttcg
      721 ctgatgactg agcttgcgga gttgcttcat cacctgcagg cgcgtcgcca gcgactgatg
      781 tccatgcgtc gggagaacaa tgcccgtctt gcggattttg cagtggcgga cgtgtcgttg
      841 ttctggttgc tgaatgccct gaacagtgcg gagcctgttc tgaaagagtt gcttgacatg
      901 ccttaccgcc acccggaact gttgtacagg gagcttgccc ggctggcagg ctccctgctc
      961 acattctcgc ttgagcataa tgtggatgcg gttccggcat accgtcatga aacacctgaa
     1021 aatgttttcc cacctttgct ttctttgctg aaccagctgc tggaggcaag cctgccttcc
     1081 cgtgtggtct tcattgaact gaaacaaaaa ggtgtgatgt gggagggggc attacacgat
     1141 gcgcgcctgc gcgaaggagc ggatttctgg ctttcagtgc gctcttccat gcccgggcat
     1201 gaactgcaga cgaagtttcc gcagctatgc aaagccggga gccccgatga tgtgtctgag
     1261 gtggtgaatg tggcgctgag cggggtcatt atccgtccgg ttacgcatgt accggcggcc
     1321 attccgctcc ggctggaaaa ccagtatttc gcgctggatt taagtacgga tgcggcccgg
     1381 gcgatgctgg acgcggggcg ttgtactttc tacaccccgg catcgctggg agatgtgaaa
     1441 ctggaacttt ttgcggtgct gcggacatga ataaaccagt tatttcccgg gctgaacaga
     1501 ttttttatcc cggctggctg atggtctgcc agctgcgcag cggacaaccg gttgaggatg
     1561 gtaaggccct gtatcgccgt gcctgtcagc tggtgaaaca ggcgcgtgaa gagctggccg
     1621 aagcaggatt cagtcaggaa aacagtgaca ccatgttgta tgccttctgt gccctgctgg
     1681 acgagagtgt gctgaaccgc gaaaaaacag acgatggctg gcacacctgg cagcaggacc
     1741 cgctgcaggc tcattttttt ggtacgctca atgccggtga agagctctgg gagcgtattc
     1801 gtgagcaact gaaattacca gcgccggatg tggcggtact gacatgcctc tgccgtacgc
     1861 ttcagctcgg ttttaccggt cagtaccggt cgcaggatga tgagcgtcgc gaagatgtaa
     1921 tacgcgcact gacagcgcgt gttcctgcat ttactttcgc acaggatgcg ccggtggtgg
     1981 tgcgtgcacc ggggtaccgt gctggcagaa cgatgtactg gttgtcatgg gtggcgggta
     2041 ttatgacgct ggctggtctc tggtgtgtgc tttcttctgt gctcgcagac caggtggcac
     2101 ggcttaccgg gcagggataa ccatgcacgt acgttcccgt ctctggctga caatactgag
     2161 tctgttcctg acactgtggc tgatttcggg attcctgcca ctgtctgcag taaaccagat
     2221 aatactgacc ctggtttcag ttgtggcggc ggggcttgtg ctgtggtatc agtggcgttg
     2281 tctgtcttcc cgtcgtgata tcccggagaa tgccggcgat gacggtctgc cgcctgagta
     2341 ttttcagggg catgtgttgc ttgttgccgg aaactcagat gcgtggttcg aacccggtca
     2401 ggcataccgg gaaacagcat cgggatggta tcttcgtgtg gatacgccgg agcagatgag
     2461 aatactggca gagcggttgt cgtacagccg tccggccctg tgtggccagg tcgtggtcat
     2521 gctgggactt ttaccggagc agcatacctc agatgaagct ttcctgtcgt ggttgcggaa
     2581 ctggcagcgt gcagtcatgc agtgccggca gtggctgaat accatgccgc cggtgctggt
     2641 gacattgatg gtttcagggc cgtcaccaca aaacacatcc ttaaataagc actctgcaca
     2701 ctggtttaca ctgacaccgg acctgtcggg aatgcacata cgcctgacgg aagccggagc
     2761 cattccggca gaggaatggc tccgggagtg tgatgcagcg tccaggctcc ggtgcgtcag
     2821 tgaactgtta tggctgaatg ccatgttgtc ctggtacaac cgggttactg atgccctgac
     2881 agatatttgc cggcaggagc acttttattt ccggccggtt gccgttggat tctgtctgac
     2941 gtcggttgcg gcccggcccg gtaatctctg gcaacaacaa ctgacgtcac tcactgtact
     3001 gccaccgcct gccggcgtgt catcacaggc actgccgttg ccggatattc tgctgtccgg
     3061 tattccccgt catcgtggac tcagtcactg gcacagactt tgtctccaga cgggggcgat
     3121 tgtcggcgtt tttcttctgc tggctatgct ggcgtctttt atgaataacc agcgactggt
     3181 tcgcagcgtg ggggaacacc tgacggcctg gcaccggatg cgcggatatt catctgaatc
     3241 gaaaaaaact gcactgcaac gtcttcaggc tgacagccgg ttactgaacg actggcagca
     3301 acatggtgtg ccgcagcgtt acggactggg attgtatcag ggaatgtggc tgataccgcc
     3361 ggttgagcga gcgactggtg agtgggttgc accactgcct ccccgtccgg tgatacagaa
     3421 agttactcag acgccgaaaa cggttcgcct taacagcctg gcgctgtttg atgccggtaa
     3481 atggacgctg aaaccgggcg ccacaaaatg gctggtgaat gccctggtgg atattaaggc
     3541 aaaagcgggc tggctgattg tggtcagtgg tcacacggac aatacgggtg acccgcagcg
     3601 caaccaggcc ctgtcactga agcgcgcaga ggccgttcgc gactggatgc gggatacggg
     3661 cgatatccct caaagctgtt ttgccgtaca gggatatggt gagagtcgtc ccgtggctcc
     3721 gaatgagacg gcggaagggc gagcgcgtaa ccgccgtgtt gaaatcagtc tggtaccgca
     3781 ggtcgatgcc tgtcgtctgc cggacatcac ccgggcgccg taacagggcg cgtaagacaa
     3841 cgagagggag taactttatg gcaattcctg tttatctgtg gctgcaggat gatggcggag
     3901 cggatattaa aggctccgtg gatgtgaagg accgtgaggg cagtattgaa gtggtggctc
     3961 aggagcactg cctttacatt ccgacggata acaataccgg taagctgacc ggtacccgta
     4021 ttcacacgcc gttcctgttt accaaggaaa ttgattcttc cagcccctac ctttacaagg
     4081 cggtgaccac ggggcagacc ctgaagtctg ctgagtttaa gtggtacaga atcaatgatg
     4141 ccggtcagga agtggagtat ttcaatacga agcttgagaa cgtgaaggtg gtgaaggtta
     4201 acccggtcat gcacgacatc aagaatcccg cctacgagaa gcataaccac cttgagcagg
     4261 tggagctgcg ctacgagaaa atcacctgga cttataagga cggtaacatc attcattctg
     4321 acgcgtggaa tgaacgtacc actgcgtaat tctcccggac gggcagggta tcctgtccgt
     4381 catttttctt ttttcttcag tatctgctgg cgggtgctga agaaagaatt ctcagtcctg
     4441 aacacagacc tgatgcgccc gggttacggc gaagggcggt agcgtgcctg acggtctcct
     4501 gacagtgagt gaataacatg gaaaatccgg cagccctgtt acgtcgtctt aatcattact
     4561 gtgcccgtgc actggaaggc gcagcctccc tttgccagac ccgggcccat gcagaaatca
     4621 ccccggagca ctggttactg aaactgctgg agcaggggga aggagacctt accgtgctgg
     4681 gccggcgtta tgactgggat atggacgcga tatggcagtc gctgctcggc tggctggata
     4741 accagccccg tagcgtacgc agccgcccgc agcttgcgcc gtccctgaat gccctgctga
     4801 aacaggcctg gatggtggcc tcattacagg gagaagaaca tatccgcagc gtgcatctgc
     4861 tgggtgccct gacggaaaat ccgcacc
//
LOCUS       DAZGCQ010000225         4778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000225 DAZGCQ010000000
VERSION     DAZGCQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.227"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..1762)
                     /gene="sctC"
                     /locus_tag="ACHK07_005083"
     CDS             complement(59..1762)
                     /gene="sctC"
                     /locus_tag="ACHK07_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO8105274.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYGDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPRAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(1759..2508)
                     /locus_tag="ACHK07_005084"
     CDS             complement(1759..2508)
                     /locus_tag="ACHK07_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8105275.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            complement(2854..3033)
                     /locus_tag="ACHK07_005085"
     CDS             complement(2854..3033)
                     /locus_tag="ACHK07_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO8105276.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(3100..4710)
                     /locus_tag="ACHK07_005086"
     CDS             complement(3100..4710)
                     /locus_tag="ACHK07_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO8105277.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT         1333 a         1029 c          814 g         1602 t
ORIGIN      
        1 agaaacttcc ctggctcttt ctaatacacc gacatgctct acatgaatcg ccatttcact
       61 atcctccgac gacttcacga ttcaggtaag tttttatcca tttctgcagt aattcatcgt
      121 taatttcttt attttttgcc atttgctgtg taatcgctct ggcatcaacc gcagcctcat
      181 tcgcattatt catcatgcgc tcatcgatct ctcttggctc aataagaaag acacgaacga
      241 tgttattcgc atttgttcct tcataaccaa ataatttacc aataaatgga atgctgccta
      301 atattgggat cttgcgactt tcataggtat tcgtatcgcg tgtatatccc ccaatcaaaa
      361 gacttttccc ttggggcacg cgtgcaatag tacttataag tgttctacca acctgaggca
      421 gatcatcaac attggtcttg tcggaattga tttcatttcc atcttcaata ttcaagagta
      481 attcaatttg atttcgcgca gcaaatcgtg gtagcacgct aatcatcgta ccataggtta
      541 cttcatccaa ttccgcagta cgctcgccga ctaatttagt atagaaagtg cggttattat
      601 caaatatagc aggaatattc tcctgtgtta aaacgacggg acgagcaaca acggcggctc
      661 ttcttttttg tgcaagtgcc tgtattgttg caataaactg tgtcccatca agggtactaa
      721 ttgaacctga attattaaat gatgcactta agctggatcc aatttttatc gtgcccgacc
      781 agtcagcacc tagctgttct agatcagtct tatcaatatc tattatccat aaagataatt
      841 caatgtgtcg ctttggaata tcaagcgtag ctactaactt ctcgataaaa tcaacctgag
      901 atactgttcc tttaacaagt aaactgttgg tatcaggata tgcaataatt tgtatatcgt
      961 cagtctctgg cgctcctgct gtcgatatta ttttctctac ttgtaggtca tcatcttctt
     1021 cagccctagg aaaaggaggc atacgtgata cgttttttag ttgttgacga cttgaaattt
     1081 cagaaagtac gttcacccct gtgctttgct taatgttatt atttaacaac gtcgatagta
     1141 cttttgccat gccaggaata actatctttt cgccacgtaa ctcataagtc cggtcattaa
     1201 cgaatgtgtt gaccagatga attattccta ctttatttcg accgatctca atgccatcgc
     1261 tgttttgctc cattaatttc gcagcattaa cgaccaggtc aacataaacc ggtggccccg
     1321 atacatagaa agtaccattg ccgccccctt taatttcata acgagagtta tacagacccg
     1381 atttttttaa gaaattatta aactcattcg tcgatacctt tctcagatta atcaatgcat
     1441 tacgcatctc tgatgagtca tagatataaa tagctttgcc atcaccatac caaataagcc
     1501 ccatctgtgc cgacaggcgt tcaatcaagg catagggttc tgttaaatca aaatttccgc
     1561 tgatctgttt cttcatggca agcttactaa ctattgtagg cttacccgca tatgatgaaa
     1621 ccgtttcaaa aaaactacgt agattttctt tatttgctac gtatccattc gctgctgcgg
     1681 catatgcacc aggagtcaat aatccattaa aaatgcataa gactgaaatt aatataatag
     1741 taatgcgtaa ttttattttc ataacttttt acatagttct ctcggtgttt ttcctaaccg
     1801 gctttttact tcggctgaaa agtgtgatga ggacgagtaa ccatatttat gcgcaatagt
     1861 tgtcatatca ctgtttcctt caatgatttc caacacggct ctggcaactc tccagccaca
     1921 taattccgtt ttaactttac cacccaatgc ataactacat aaacggcgaa aatgtgaata
     1981 cgatacgccg taaagctcac caagatcata gagtgaagtc tgtatgagcg attgcgataa
     2041 taaataacga accagtttat agcattctgt ttttcttaga tgctgaaaca ggatttcatt
     2101 aatattatcg ctgctcagat aagtggctaa aaaccaggct tcacttgcaa ctttactgaa
     2161 tagcacaact tctgatgcaa agggcaaggc atcagcaaac aattcatgat gttccctttc
     2221 cacactcccc gttgattcat ctataaaagc taataattta ctcaggatat caatgcgaag
     2281 tggatgataa attactttcc ctgcagttac ctcaatttgg cttcggtcaa tgagcagtaa
     2341 tatatcttcc actaccatta atttttgatg gttattacca atcgtaataa caagggttac
     2401 atccttgttt tcatttcccc gtaagaacca catttcaggg tccagtaggg ttaacttctg
     2461 tccttgaagc aaaacattca ttggaagtaa cagcccttct tcaatcatgt ttcatctcct
     2521 tattccatat tctcattctc cataaacttg ctataaaaac atgattaaaa agtgatgatt
     2581 actatttata aaataatgat tttgttatga gcgaatagct atcagccact tacactagta
     2641 cgtcatcctg tccatataca gatttgtaac tgttgatatg ttttacttaa acacacctcg
     2701 tatttcttat aacacttgtt tggtaactat tttcaataca aatcatattt gttagttctt
     2761 cattaacaca ctgcgagtgt tttgttactc cccgcatata ttttattctc ttgttcgatt
     2821 ctatttttct taatcgatca tataattttc ttgttagtcg gatttacgaa tcatgagact
     2881 tataatggaa ggaatgagac taagcggcca ggttaaacct gtaattattg cagcaaacaa
     2941 ccgatatgtt actttttctt ccgagaagat aaatattaaa gtcgatgtca caaccagacc
     3001 tatcaggtaa gctaacagta atttattgat cacttttacc tcccctttcg atttctcttt
     3061 gagcggtcct tttaatccag gaccgcctca ttatgaaaac tatttaattc cctcatcatt
     3121 gataaggttg gacatctctt tcaacatgag agtcattgaa gctttcgacc aatttagcgg
     3181 aatgataggg cttggcgctt catgcaaagt tcccgacttg tggatatagt tatagctttc
     3241 gggaagtgcc atttctggta cgctcctgcc atttgcacca atcatattct cgcctgttat
     3301 ttgggccaat gatctattca taaattgtac tgagtcattc aaatactctt cttttcttga
     3361 ttctttataa acaattgctg ctgattttgc ataccatgag tcaaaaaacc attctgcttc
     3421 tgttgatgga atgaagctct tttctctttt tgcatgcgaa ttttgatcgg tatcggtttt
     3481 aatatcattg aaccaaaaat ttgccgactg ataattatcc ttctcgtatc tttttatacc
     3541 atacggacct gctagttttt taactatttt tagaacttgc tcttttcttc tggtgtttat
     3601 ctttgaaaga tttgcaggat aaataacatt aagtaaagcc gcatcggctt ctctgtaatg
     3661 tttatctttt tccagataac ctggggattc tccccctaaa tcaagctgta acgttattct
     3721 ttcataacct ttatctatga ggtggttcaa cctcgttgtt gaaagagttt catccagttc
     3781 attgacttta gcttcgcgta gtaaatcaga gacaaaaaca gagtctttct tcgagagtag
     3841 gtttgacaaa cgttccagtc cagaggtcac cagtgcaacg gaagatgtgt ttaatcgggc
     3901 atcttcctcc cacgctccgg aatcttccat aacatagaaa ttggctttat ccagataagc
     3961 aatcagcagc gcaaccgatt ttagtcggtc acctttttgc caatcttcag cgttaatagt
     4021 accagtattg attgcttgta taaggagatc aagataaaga cctaacgcat cattttgttt
     4081 atggttccac agctgcggct tgccttcttc ctgcacatct gccatcacgg gggagttgct
     4141 gtcaaatcgg atatgcacag cattcatttg cccgggaata ccatccagtc gttttgggtt
     4201 gctaatgatg tcctgcatcc gcttaatctg gtcgggcgta gacatataat cccacagggt
     4261 tagcagaacc ttttttgcgg ctacactatt tccctggtct gatactaatg ccatatagcc
     4321 ccataaacta tccctcagcc agacagcatc atagtgggtt tctgccgctg ttttgtcatc
     4381 ttcgaaagta gaagcaagaa taaacgttcc ccagggtttc tccgtcaacg ttaactttcg
     4441 tgtgttttct gttaatgtta gcaatccttg aatttcgtct ttggtatagt ggctctttat
     4501 ttcattttgt aatttggctg agtgaatcag aagacgataa ttaggatact tatctggatt
     4561 taacgataaa taacttttat ctgttgctgc ccccgtggaa ttaagaatgt taaatacaga
     4621 ttcactcgtc acttctgccc ctgtcgcctg gcatgtgccg cacgaaaata taccagcgag
     4681 cagaagagtt aatgcagatt tccctttcat aaaatattcc atcaatcgtt tgttaaaagc
     4741 gtagataacc acttatttaa caacttgaaa ataaggta
//
LOCUS       DAZGCQ010000226         4766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000226 DAZGCQ010000000
VERSION     DAZGCQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.228"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..390
                     /locus_tag="ACHK07_005087"
                     /pseudo
     CDS             114..390
                     /locus_tag="ACHK07_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(499..4014)
                     /locus_tag="ACHK07_005088"
     CDS             complement(499..4014)
                     /locus_tag="ACHK07_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO8105278.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSIDFDDSVDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGVMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKEFSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQFQIQLFLEDKLGALIAGNQWSDPETIERNIDELLYSAECEQTTYRKQIDSA
                     HEIFLKEQQAVQEWQRLALDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSASTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGRENSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDNFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
     gene            complement(4466..>4766)
                     /locus_tag="ACHK07_005089"
     CDS             complement(4466..>4766)
                     /locus_tag="ACHK07_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8105279.1"
                     /translation="TVNKALRVMGYDTTREVCGHGFRAMACSALIESGLWSRDAVERQ
                     MSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREECISPFEYAKINNPLK"
BASE COUNT         1250 a         1183 c          981 g         1352 t
ORIGIN      
        1 ataatttaaa atttattttt tgaaataaat aatgtggtat gataataaat ccaggtcagc
       61 agtaactggc tatcgtgtat aattatccag aagtcctgct gagccttccg tctgttcatc
      121 atgtgctagg tttgcgttcg ggatatgatg tgccatctta tgatgatggc aagagatatt
      181 actgcgtacc acctcaattc tgtacacatc agtgcccgtc gtttccccag gatttttcag
      241 tgggccaccg gtattcgtac cgaaaatata ccgccattca atccgctgac agacgaagat
      301 aatgaacccg ttatggtttt taacggagag aaaaatgtgc cggcagacag tctgaaaccg
      361 cagtctttga ttcttccgga cagaagataa tcatgttggt atgaatgtta taattaagga
      421 ctgtgcaata atagctattc cattcagcca tcttacgctc gaaaacgatc gataagatgg
      481 ctactcacca ttcctgctct aaacctgacg cttctcaaga ctattataaa tagcctcaat
      541 ctcacccttc aggttatcac cgcaataaac cattgaggta tcgtatcgtt catatcgcgc
      601 ttctcgcgtt gcgctaaacc agttgaacga tcccacgcac agcaaaccat catcaccaat
      661 aacaattttg ctatgaacgc gatttaccag ctttgtagta ataccaaaag cattcagttt
      721 ttccagcgtc cctttaagat tctgctgctt ctcttttcgt ttctcataat ctttatgttc
      781 agtgttgtag cttctgtcag tgactatcgt gacgttaata ccacgtgaac acgccgcaat
      841 catggaatca agaaaaccgg tttgctccag cttttgccag gtcagccatg gagaaactat
      901 cgtgatgtat ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      961 accatgaagt gtgtagattt tggtcccgga ggtttttaaa tccttacgct ctttataatc
     1021 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagcaatc ctcgcggaga
     1081 tgagggcggc tggacctcaa acagatccat atcgccgaag accaggaagc tatccttcgc
     1141 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
     1201 ttttgaatag actggcgaga ataccacaat cgctctttcc gctccctgaa gagagtgcac
     1261 ggtgcccact gtgagcgact tttcattcgc gccagtactg ataccttgtt tacccagcgc
     1321 ctgtttgata gtggatacct gcgcgctaaa aggcgtcacg ataccgataa cttcatgaag
     1381 cgatttgccg taatgcgctt caatattttg ctggttctct gccaaccagg ccgctattgt
     1441 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1501 atggagatac cccattgcgg gcattaaatt gctattttca cgccctcttt taggcaacaa
     1561 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1621 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1681 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1741 tttctcggta atctcttctt gcgtactggc agacagaatt ttttccgcaa gcatgttacc
     1801 gacatcaatc gcgggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1861 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1921 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1981 tcctttgtgc tcacttatct gcatattgcc gggcagcata tagcatgtca tcaccacaca
     2041 tggagtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcgc cttttttctt
     2101 cttctcttcc tgcagatcat caatactggc catatccatc aaccaacgac cttcccagta
     2161 gtgagtcgtc agtaaaaatg cagggaagcg aatctgtgta tcagccagtt catcggcctg
     2221 tgagaagctc agttcctcgt cgccctcata ccctaaatca agtgccaacc tctgccactc
     2281 ctgaaccgcc tgctgttctt taagaaagat ttcatgggcg gagtcaatct gcttccggta
     2341 tgttgtttgc tcgcactcag cggaatagag tagctcatca atattacgtt cgatagtttc
     2401 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2461 tatttggaac tgtcgcttac tacgaaccgc cgggagccag gaaaataatg aatagatcag
     2521 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2581 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tgtcgttagc
     2641 aataagctca cgcgcagccc gtacctggct taacgtttgc catgttgcgt tcagtcttat
     2701 cagttgctcg gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2761 tgaaaactct ttttcaggaa aagctgcctt agctttctcg agataaaaca gcagagcatc
     2821 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2881 tgcctcagct ttacgactgc ttgagggaaa ataagcgccg aagcttttca gctctggcaa
     2941 ccatcgcccg gccatcacac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     3001 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     3061 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     3121 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     3181 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     3241 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     3301 attgaagagc ggcacatcct tcttgcaaac aagcaggtga tcataaagcg aaaggatatg
     3361 gaaactggca ccgccagatt gcgccgtttt aacaatataa ccgtgctcag cgagttcata
     3421 ttgctcagga tttttaatcc agtcgccggc aacgttcttc agcagccttt ctgaatcatc
     3481 cagatattga cgccactcct gctgcaaggc tgcatatcgt gcttcccgtt cttcatccgt
     3541 ttcggcagtc ttatcaacac tgtcatcaaa gtcgatagag aacgaggtat ggattgtctt
     3601 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3661 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3721 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3781 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3841 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3901 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3961 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcattttcat ccattaccag
     4021 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     4081 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     4141 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     4201 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacga ggataccaga
     4261 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     4321 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4381 acttgtcaga acatcaatct tcgtcatcat tttcaaagaa aaataattag ttaaagcagt
     4441 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4501 acggactgat acactcctcc ctgtttgcat ccagaaaatc tgcccaccac tgtagcatca
     4561 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4621 cctgatgact catctgacgt tccactgcat cgcgggacca caaacctgat tcaatcaatg
     4681 cactgcatgc catcgcccgg aatccatggc cacagacttc ccgggttgtg tcatatccca
     4741 tcaccctcag ggctttattt acagta
//
LOCUS       DAZGCQ010000227         4764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000227 DAZGCQ010000000
VERSION     DAZGCQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.229"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK07_005090"
     CDS             complement(<1..275)
                     /locus_tag="ACHK07_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8105280.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
     gene            complement(275..604)
                     /locus_tag="ACHK07_005091"
     CDS             complement(275..604)
                     /locus_tag="ACHK07_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105281.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(601..3246)
                     /locus_tag="ACHK07_005092"
     CDS             complement(601..3246)
                     /locus_tag="ACHK07_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8105282.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKDVPGVSALFGELAEENREFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISQQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEY
                     KRQLAELGDKVEYQKRLNELAQQAARFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWTESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASTGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3296..3598)
                     /locus_tag="ACHK07_005093"
                     /pseudo
     CDS             complement(3296..3598)
                     /locus_tag="ACHK07_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3625..4047)
                     /locus_tag="ACHK07_005094"
     CDS             complement(3625..4047)
                     /locus_tag="ACHK07_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8105283.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(4063..>4764)
                     /locus_tag="ACHK07_005095"
     CDS             complement(4063..>4764)
                     /locus_tag="ACHK07_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105284.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAESYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          926 a         1594 c         1123 g         1121 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ctggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcgat ttcccagagc accacccgcg gtgactgctc tgatttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatcagtt caccacctgc ttaaactccg
      301 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag gtcaccttta
      361 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagcccccg tgctctgcca
      421 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt
      481 tcaggtcagc attcagccct gccgccatac gctgtgagta cccgtcacca aaacgcactt
      541 cacgcaccga cggctgcgag ttcacctcca tatccggctt cactttccag cgaaaggttt
      601 tcatcgcccg ctccctgata acataccgcc atcacgcaac tgcagccgga gctcatcctg
      661 tgccccttta cgggccatgt catacaccgc cttcatcagc tgcggccccg cctgtccgtt
      721 gataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatac cttccgcccg
      781 ccgcatctgc gccggacttc cggcaccgcc gacataacca ccttccgcat agccgcgcat
      841 caggcggtaa agattcccca cacctatccg gctggttgcc tctttcgtga aaacaaactc
      901 cccgcggtga actatccccg caggctcata tttgccgccc gtccccgtaa atccgccggt
      961 cgcaaaatga aagttcgccg ccgcagcctc aatggccgtt cccgtggaag cggatgcgcc
     1021 accaccgaaa gcaccaccaa tggcgctgcc gatacgcccg acaattccca ccatggcctg
     1081 tttaagcagg atttctgtca tcatggacag caccgaacgg gtgaatcccc gccagtctgc
     1141 ctctgcaccg gtcagcatcg ccgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc
     1201 agcactttta acctgcgaaa aactgtccgt cgcactttcc gtccactccc cccagccgga
     1261 cttcagcccg gccagccagt caccgcgcag catgtcttca tccgcccatg tctgtttcag
     1321 tgccccggtg acctgtgcca gcgcctgcgg attatcgcca tacacgtcac gaagacgctg
     1381 ctcttcagac tcccgctgcg cctgacggtc agtgagacca cgggcttttg cgctgatggc
     1441 ggcctgcttc gcgctctgct gctcttcaaa ccgtgccgcc tgctgtgcca gctcattcag
     1501 gcgtttctgg tattcaacct tgtcgcccag ctcagccagc tggcgtttgt actccagcgt
     1561 ctctttctca tgggccagca gggatttttc ctgcccggat aactgccgtt tcgtggcggc
     1621 ctctttcagg accgcatact gactttccgc tttccataaa tcacggcgct gctggctgat
     1681 tttctcattc gcaccgctgt gcttctccag cgtcctgagc tcagcttcaa gggcaagcag
     1741 agcctctctt gcctgctcct cttccctctc cccggcagag cgcgttttcg gtgatgtatg
     1801 ctttttacct gtcagctctt cagccagacg gctgacggct tcctgctgcc ccggaccttt
     1861 gctgacgcct gttgcacgcg agcggttgat gtaccccatt tccccctggc gtatacgcgc
     1921 atcccgttcc gcaatggatt ttctcagcgc cagttcatcg cgttttgttt tctcaataaa
     1981 ttcccggttc tcttctgcca gctcgccaaa cagcgcactg acgccgggga catcttttgt
     2041 cgtttcccag gctgactgaa taaattcagc cagcgccaga tccccctgca caagcagcag
     2101 cttcacctgt tcaacggttc ctgccaccac gtcggtgatc agactgaggg cccccagagt
     2161 atgatcacct atccatgccc atgcgtcaga agtccaggtt ttaacatcgt cccagatttt
     2221 ttccaccggc gtggccgctt tatcaagttg ctccagacgt gcattcatga catccgcaaa
     2281 cagggacatc gcctccgtta ccgcagcctg tttacctttc gtgcgctcaa gctcatcaat
     2341 atggcgtaac tgggaaacgc tcaggaagtt atactgctga ttcagggagg ccagcgcctt
     2401 caccggatct gctgcaatcc cttcaaaggc tttttccacc ttcccggcat cgtcccccac
     2461 cgtctgcagc catctctgag aggtttcccc catgatccgt agctgcccgg cggtatattt
     2521 cccgctttct gccagacggg ccagattttc tgccgcctgt ctgataccac caccggcttc
     2581 atcgctgatc accccggcca ttttccacaa ttctgccgtt gtggtggcag ccgcccctcc
     2641 ggtcagggtc agtgaacgca acaaggcccg gtcagcctgc tctgcctgcc aggcggcggc
     2701 agcaagcgcg gccagtacgg caaccccgcc acctgccgcc acacgggcca ccgacataaa
     2761 tcgtcccagt tcaccggcat tccgggcatt ttcagccagt gcatttgccg tatctgacag
     2821 cgaatcctct gatgattcag aggcatccct gatcccgaga agttcctcct tcagcagggt
     2881 aagcaggctg agcggtccac cgaatgaatc gctgatctgc cccccctgct gcagcatgat
     2941 aaggaaggga ttctgaccac cggcaagctg agtgacaata tccgtgaact gtgcgggcag
     3001 tgtgcgcatg gcagccttat actgtccgac tgatatcccg gctttttgtg cagccagcgc
     3061 ctgtcggctc agactctgct cagcagcatc agcctgtttt ctgaaagact gactgacttt
     3121 cccggacatc aaatcagcaa gcgcactggt ttcacccagc tcttttttta cccgcgctgc
     3181 ctcttcagaa aaacgggcag aatccagtgt cagcacggct gtcagatcgg caaaattacc
     3241 tgccatcgtg gtcacctcct gaaatgtcct ctgatgccat caataactgt cacaacctcc
     3301 ttccctcctc cccgaaacgg actccaccgg cgaggcccgc cgccttctgc atcagtacat
     3361 cattttcgtc cggcgtctcc gcctgccctt cctctgcctc cggagcgaac aggctgaaat
     3421 ccgccggatg catatccgga tcgcaaaaaa acaggctgag tacggcgtac gtcagcccgg
     3481 aaaaatgcat atccagctgg gtatcctgaa aataatgcgt gcagtaaaaa cgtcgccagt
     3541 cggcatattc ggtggatgtc atcccggcaa gcatggcgcg ccagtcggct ctccccatct
     3601 cacgcgccag tttcaggaca aagttcagct cgccttcgaa tgcttttttg atgttaccgg
     3661 ctcagtcgct tctgctttcc cggttggttc aggatcggca tcgtgccggt tatccagcat
     3721 acctgaaaga taaagcaccc ggttcgttgc ctgattcagt gcatcagcag gccatcccag
     3781 catcacttca cggcggatct gctgcatctc tgtctccgga gaggccagag tgcctttgag
     3841 agaatgggaa tgccatagcg acatcgccac aagcagggat gccgtttcca gatatcgctg
     3901 gttaatgtgc acggcatcat gctccgttgt ctcctgttgt gctgcgtctg aaacaaactt
     3961 catataatca aaccgctgca gcgcagacag ctccgacagc gtgacggaca ccccgttata
     4021 ttcaaattgt tctgttttca gaaacatgct ttatctcccc cctcagcccg cagcgccatc
     4081 cgtgacggtg atctccgcca ccgccgcaaa ctcaccattg ccggtgacaa cagggatctg
     4141 cgctttacct gccgcaacac ctttcacggt gatcgtgttc cccttcacgg taatggtggc
     4201 gaaattctga ttcgctgagg ttgcgcggaa cgttttatcc gttgcgcctt ccggctgaac
     4261 ggccacggtc agggtgatat tctgaccttt tgccacattc cccgttagtg gcgtcacggt
     4321 aataccggtg accggtgtga tttctccccg atcctcagcc agcgacggac gaccaatatt
     4381 ggtgattttc acagtgcggg tgatcacttc tttggcggtc acggatttac caatggcgct
     4441 cacccagccc cggaacacat ccaccgtgcc gttcgggaag cggattttgt aggcccgggt
     4501 ctcgctgctg tcaaaccagg caatcaaatc acgctgccct ttctcgccgg gcttccaggc
     4561 cagcgtaaaa ctggtgtcac cggcggattt ctgcccctgc ccggtggata cccagtcggc
     4621 atcctcatca tccagatagt tatcgtcgta ggattccgcc gtcatctcgc cgggggtcag
     4681 atcctttatt tttgccagac gcgtccactc atcatctgac agcgggtttg cataagcatc
     4741 gcccttaccg gtgtaaaccc agag
//
LOCUS       DAZGCQ010000228         4759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000228 DAZGCQ010000000
VERSION     DAZGCQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.230"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1416)
                     /locus_tag="ACHK07_005096"
     CDS             complement(<1..1416)
                     /locus_tag="ACHK07_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8105285.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGT
                     LAGTTTLNNGAILTLSGKTVNN"
     gene            complement(1742..2614)
                     /locus_tag="ACHK07_005097"
     CDS             complement(1742..2614)
                     /locus_tag="ACHK07_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8105286.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2880..4436
                     /locus_tag="ACHK07_005098"
     CDS             2880..4436
                     /locus_tag="ACHK07_005098"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO8105287.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAKLDDSVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
     gene            4433..>4759
                     /locus_tag="ACHK07_005099"
     CDS             4433..>4759
                     /locus_tag="ACHK07_005099"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8105288.1"
                     /translation="MSELSYLEKLLDGVEVEWLPLGEVSALRRGRVMSKGYLTENFGP
                     YPVYSSQTANNGKIGSINTFDFDGEYISWTTDGANAGTVFYRTGKFSITNVCGLITLK
                     SKYPLIY"
BASE COUNT         1164 a         1235 c         1204 g         1156 t
ORIGIN      
        1 gttgttcacc gtcttcccgg aaagggtaag tatggcgccg ttattcagcg tggtggtgcc
       61 cgccagtgtg ccgcccctgg cggtgaacag tccgccattt accgtggtat ccgtggccgt
      121 atcaccggcg ttcagcgtga atgaactgcc tttttccagc atcagggcat ccgcctgacc
      181 gcctccgata ctgaatgcct ttccgtcgct ccgggtacca ctgacagcgg caccggaatc
      241 ggccagcagt acaccgccat tccccatgga tacggtggtg gcggcaccac cgttgcggac
      301 atccagcgtt ccgccatcat ccacacgggt attagtggct gtgtgtccgc tcagcacatc
      361 cagacgtccg ccattttcca gtacgacatt atcagcctta cccgccacaa cggagaatgc
      421 tcccaggcgg tttgtaccgg tgacggttgc agctgtactg gtaaccagtg caccgcccgt
      481 gttctgggtg acatcagacg ctttaccgcc ggcattcacc tgcagctttc ctttctggtt
      541 gatggtggta tgcgcggcag ttcctccttc cttaatcacc tgccagccat cacggtttat
      601 cagcgtctct gttgccgtgc caccgttgtg tacatactgg ttacccccct ccagccgggt
      661 gttatgtgcc tctccgtgta ccgtctggtc accaccggcg taaatgaggg tgtcccgtgc
      721 catccccgaa gaccagataa cctgccggcc atttttgttg atggtggtgg attcagccgt
      781 ccctccgacc atctgaccgc tggacacatt ttcagactcc ggaccacctt ctgcaccggt
      841 gttgacgatg gttccggttg ccagtccgcc atgttttatg gtctggtaac catcctggtt
      901 aataattgta ccggcagctt tccctccccc gtgtacccac tgctcacctc tgttatccag
      961 cgtggtgttc accgccagtc cgttaaggct ctgccctccg ccatcacgaa taaccgtatc
     1021 actggcagtt cctcctgcct gcacaatctg acgaccattt gcggtgacag tggtgtttct
     1081 gcctgtgcca cccgcgttta tccattgccc gccggtgttt tcgtcactgt ccggccccag
     1141 ctcaagcccg gtactgacag tcacgccatc agctgttccg gatacaaact ggttgtcatg
     1201 gtttaccagt gttccgccat tcactgtttc acccgggtgc acaacgatgt cagcagccag
     1261 caccgggagt gacgtgaccg cggcaagaga cagtgcaacc gccacaccgc cacgtttacc
     1321 ccgtgcgcgg gccagttcgg aggcaaccac gaaagcgccc gtaatgtgat tccataccag
     1381 cctgtagcag gtattcagat gtcgtttcat cagcatttcc ttaatcagag tgagggtgaa
     1441 taaaaaagcc ggtatccaca gatgtggata ccggcaggta aagggttaca gtgccggagg
     1501 tccggctgtg ggagtttctg aattgtggtt ctgttgttcc ctgaatgtct ttctgtacgt
     1561 atctgtgcat gttactgaca aaggttatta ttagcctatc gatcgatata aacgatcgat
     1621 aaaatagata tcgatcgttt tattctatta ttgagctatt gcacggacaa gagagacagt
     1681 aacgaaaaca gtgcttttgt gagcagactc agagtcaggg agggcatcag tcacgacagg
     1741 ttcagaagaa aatccagttc cataccgcac gggcaacaga gaccaccgtg tcacggacgg
     1801 cacgcagaac cgtgcgtgca acagaggcaa cacagacgct ctccgctgtg tcaaatatcc
     1861 ggtccaccgc accggtaaac tgttcacggg cctgagaccg gacagattcc gtgcgcagct
     1921 cgtcctgcag gcgggtcatc aggggactgg cggcatggtc gggaagcgct gtcatgagcg
     1981 cactgaccag cgtatccagt tcccagccgg tgcgggccga tacggcaaca accggatgta
     2041 cgggccggaa cagacggaat accgcctccg ttttttcgcg aatattctgt gcctgtgcgg
     2101 gagaaggctg aatgccggcc atatcccatt catggcaggg ctccgttttg tcggcctgcg
     2161 tcaccacaaa cagtacctgc tgatgtcccc ggtgcaggat gtgtcgccag aaatactcat
     2221 ccacagacag ggcacggtca tcggctttaa tcagccacag taccaggtca agttcaggca
     2281 gaatgtcacg gtacagggct tcatactctg catccctgtc ccggctctcg cccaccccgg
     2341 gcaggtcagt gataaccatg ctgtgaccat gtccactcag acggaagcgc cgcacttccc
     2401 gggtgccggc gtgaacatca ctgaccgggg tgacctcccc ctgaaacagt gcattacaga
     2461 gtgaggattt accggccccg cttttaccca taatgccaat cacgggttcg tgactggtga
     2521 gtttgcgcag atgttccagg atgtgacggg aaagtgagta aggcagggag gagagcggtt
     2581 tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccggtg aaaaaagaca
     2641 aaacccccgc acaccggaaa ggtggcgggg gactcacgga gataatatct gtctgaaatt
     2701 attttgaatg tgccggtcgc acagcaaaga aggggcgata ttctgcacat cctcccggtt
     2761 atgaataacc cggagtatct caatgtctac cgacttaccc cggggctgtc tgaaccaccg
     2821 gattactgta gaattgcccc tgatgactca ggtctgaacc aggaaaaaac ggataaaaaa
     2881 tgacaagtat tcaacaacga gcagagctgc accgccagat atggcaaatt gccaatgatg
     2941 tcagaggttc tgttgacgga tgggatttta aacagtacgt tctgggcgca cttttctatc
     3001 gttttatcag cgaaaatttc tccgggtata tggaagccgg tgatgacagc atctgttatg
     3061 cgaaactgga tgacagcgta attactgatg acattaaaga cgatgccatc agaaccaaag
     3121 gttacttcat ctacccaagc cagctgttct gcaacgtggc tgcgaaagca aataccaatg
     3181 acagactgaa tgcagattta aacagcatct tcgttgctat cgaaagttct gcttacggtt
     3241 atccttcaga agctgacatc aaaggtttgt ttgcggattt cgataccacc agtaaccgac
     3301 tgggtaacac cgttaaggat aaaaatgccc gactggctgc ggttctgaaa ggggttgaag
     3361 gattaaagct tggcgacttt aacaaacatc agattgacct gttcggcgat gcctatgagt
     3421 tcctgatttc taactatgca gcaaatgccg gtaagtcagg cggagaattc tttacaccgc
     3481 agcacgtctc taagctgatt gcacaactgg ctatgcacgg gcagacccac gttaacaaaa
     3541 tctacgaccc ggcagcaggt tccggttcgc tgttgttgca ggctaaaaaa cagtttgata
     3601 accatatcat tgaagaaggc tttttcggac aggaaatcaa ccatacgacc tataatctgg
     3661 cgcgtatgaa catgtttttg cacaacatca actacgacaa gtttgatatc aagctgggca
     3721 atacactgac tgagccacac ttcagagatg aaaaaccgtt tgatgccatc gtttctaacc
     3781 cgccgtattc ggtgaaatgg actggcagcg atgacccgac actgattaac gatgagcgtt
     3841 ttgccccggc tggcgttctg gcccccaaat ccaaagctga ctttgcgttt gtattacatg
     3901 cgctgaacta tctttcggcc agaggtcgcg ctgcgattgt ctgcttcccg ggtatttttt
     3961 accgtggcgg cgcagagcag aaaatccgtc agtatctggt cgacaataac tatgtcgaaa
     4021 ccgtgatttc actggcaccg aacctgttct ttggcaccac cattgccgta aacatactgg
     4081 tgctgtctaa acataaaacg gataccagag ttcagtttat tgatgccagc gaactgttca
     4141 aaaaagaggc taacaacaac attctgaccg atgctcatat cgaacagatt atgcaggtat
     4201 ttgccagcaa ggaagatgtt gcccatctgg cgaaatcagt cgcgtttgag accgttgtcg
     4261 ctaatgacta taacctgtcg gtgagcagct atgtggaagc gaaagatact cgtgaaatta
     4321 tcaatattgc tgagctgaat gctgagctga aaaccacggt cagcaaaatc gaccagttgc
     4381 gtaaagatat tgatgcgatt gtggctgaaa ttgaaggctg cggggtgcag aaatgagcga
     4441 gttgagttat ctggaaaaat tgctggatgg ggttgaggtt gagtggttgc cattggggga
     4501 agtctcagcc ttaagaagag gcagagttat gtcaaaggga tatttaactg aaaattttgg
     4561 cccatatcct gtctatagct ctcaaactgc aaataatggg aaaataggtt ctatcaatac
     4621 cttcgatttt gatggcgaat atataagctg gacaacagat ggcgcaaatg caggcactgt
     4681 gttctatcga acaggtaaat tttcaataac taatgtttgc ggcctaatta cattaaaaag
     4741 taaatatcca ttaatctac
//
LOCUS       DAZGCQ010000229         4631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000229 DAZGCQ010000000
VERSION     DAZGCQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.231"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHK07_005100"
     CDS             complement(<1..617)
                     /locus_tag="ACHK07_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8105289.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(732..807)
                     /locus_tag="ACHK07_005101"
     tRNA            complement(732..807)
                     /locus_tag="ACHK07_005101"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Thr,seq:ggt)
     gene            complement(814..888)
                     /locus_tag="ACHK07_005102"
     tRNA            complement(814..888)
                     /locus_tag="ACHK07_005102"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gly,seq:tcc)
     gene            complement(1005..1089)
                     /locus_tag="ACHK07_005103"
     tRNA            complement(1005..1089)
                     /locus_tag="ACHK07_005103"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Tyr,seq:gta)
     gene            complement(1098..1173)
                     /locus_tag="ACHK07_005104"
     tRNA            complement(1098..1173)
                     /locus_tag="ACHK07_005104"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Thr,seq:tgt)
     gene            1535..2485
                     /gene="coaA"
                     /locus_tag="ACHK07_005105"
     CDS             1535..2485
                     /gene="coaA"
                     /locus_tag="ACHK07_005105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO8105290.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHK07_005106"
     CDS             complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHK07_005106"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO8105291.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHK07_005107"
     CDS             complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHK07_005107"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO8105292.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGRVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1168 a         1170 c         1058 g         1235 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aagaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcacatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcgcgagcag caccgccgta ggttttagcc agtacggtag taattgcagc ggtcagcgtt
      541 gttttaccat ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca
      601 aacttttctt tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac
      661 atcaaccagg caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag
      721 gagaaaagaa gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt
      781 gcgctctacc aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg
      841 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc
      901 ggctacctga ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt
      961 aagcgaactt atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg
     1021 attcgaacct tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg
     1081 aaccccaccg gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca
     1141 agtcagttgc tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac
     1201 atgcgagttc atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga
     1261 cacgatgaag aaacagccga atagagggca gattagcaca ctttttcaac atcattgtgc
     1321 tcaacaatgc gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt
     1381 gcttttcaac gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt
     1441 taccctcctg cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt
     1501 tgccgataga gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat
     1561 gacgccttac ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat
     1621 gacgttatcg gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga
     1681 agaagttgcc gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa
     1741 tctgcgccgt caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta
     1801 cattatcagt attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca
     1861 ggcactatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt
     1921 ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga
     1981 atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa
     2041 cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac
     2101 tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga
     2161 ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt
     2221 tgatgctccg gaagacttac ttcagacgtg gtatatcaac cgttttctga aattccgcga
     2281 aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga
     2341 agctattaag actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa
     2401 tattctacct actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt
     2461 agaagaggtc agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt
     2521 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc gtcctgctca
     2581 agcaataaag cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     2641 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa
     2701 ggtgccaatc cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg
     2761 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     2821 ttaacgacac tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt
     2881 tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc
     2941 tgcagataga ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat
     3001 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     3061 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccgc
     3121 ccccggcgac cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc
     3181 tctccgatac gatcaagaag gtactgattc gtggagtcaa taactggcag cacggctaca
     3241 ctaccgccat ccagctgacc caatatctgt tcagcattaa gtaactggat gggctcaggc
     3301 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     3361 atgtgtttat taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag
     3421 tgaaattcgc cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg
     3481 aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa
     3541 gccagacatt aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat
     3601 cttcgctttt tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcag
     3661 ccccacccat ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt
     3721 ttactgaacc atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt
     3781 tagccgtttc agcagataca acagggtttt tgaagaaact accggcattg ccattcactt
     3841 ttggatcagg aagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg
     3901 ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt
     3961 ttggcagacg cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa
     4021 tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg
     4081 ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa
     4141 tattctggat aggtgatgag ccgacacgac caggaattaa tgccagattt tccagaccag
     4201 gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat
     4261 gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca
     4321 ccgtgccgcg atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg
     4381 gttgcccttc tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac
     4441 atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt
     4501 tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc
     4561 ttgtagttat gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg
     4621 ggccttctgc t
//
LOCUS       DAZGCQ010000230         4543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000230 DAZGCQ010000000
VERSION     DAZGCQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.232"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1403
                     /locus_tag="ACHK07_005108"
     CDS             <1..1403
                     /locus_tag="ACHK07_005108"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO8105293.1"
                     /translation="NKYLLLVRRYLNNNINIESYNMIGLIISLILLIYLIYRGFPVLI
                     VSPLMAMLAVFMGSDIPVFYSWTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASGAAL
                     TIAHFISDKMGEKRAIIAVIIASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQRLL
                     PSAICAGCLPAYVAPGSAQFINAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYMSAK
                     IKNAKIKGEGYGTEVSNDCNLETGKVTLAFIPILIVIIGNAFLTYFFQIDEVVDYYRD
                     FGGVQGTWAILISIAIAIVVTICIYWNTFKSDIKKTLTLGATESLLPIFNTSTQVGYG
                     GVIKVLPVFTLIQTTIFSIPGVALFPAIIGTAILSGIVGSTSGGVGLVMVTFGQDLLE
                     LSQAQNISPGLMHRIIVFSASTLDTLPHSGFIITLLGVCGLSHKQSYKELFIVTCVFP
                     AFAVLSMLVYAFLFLI"
     gene            1416..2981
                     /locus_tag="ACHK07_005109"
     CDS             1416..2981
                     /locus_tag="ACHK07_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8105294.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDCAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKIIARRAAMELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTP
                     AGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGC
                     GGFINITQNAKKVYFCGSFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDTA
                     RKNNKEVFYITERAVFRLSQKSIELIEIAPGVDLQKNIIECMEFVPEISPSLKIMDER
                     IFLEQPMGLELKD"
     gene            2984..3400
                     /locus_tag="ACHK07_005110"
     CDS             2984..3400
                     /locus_tag="ACHK07_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFO8105295.1"
                     /translation="MVYTIDKLHLGQKASFEKTITEADVLIFSGISGDINSVHINEEA
                     AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRKPVFIGDTVKATCEV
                     VDINKEKNIVKLSTITTNQNDEVVIEGIAVVMAPKS"
     gene            3426..>4543
                     /gene="fabV"
                     /locus_tag="ACHK07_005111"
     CDS             3426..>4543
                     /gene="fabV"
                     /locus_tag="ACHK07_005111"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HFO8105296.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGTAGWYNNIFFKEFAEA
                     EGRIAHNVIGDAFSDDVKNKVLNLINKDFDGEVDLIIYSLATGQRSDPATGNIWRSCL
                     KTTGSAFTGKTVNIEDNKLESTIIDVASEAEIADTIKVMGGDDWESWILWLKENNVLT
                     KKTKTIAFSYIGPEMTYPIYHTGTLGKAKQHLHETSDRLNKLISTDKHSGAYVVVCKA
                     LVTKASVYIPCFSPYILCLYKVMKSMGLHETCIGQMHRLFTEYLYNVDNVVVDSSRLI
                     RIDDLELREDVQQKVIDLMSKMNDENFTSVGDYIGYKK"
BASE COUNT         1433 a          670 c          952 g         1488 t
ORIGIN      
        1 gaaataagta tctcttgctt gtcaggagat acttaaataa taatattaat attgagagtt
       61 ataatatgat aggcttgata atttcactta ttttactaat atatctcata tatcgtggtt
      121 ttccagttct gatagtatcc cctttgatgg caatgctggc tgtctttatg ggaagtgata
      181 ttcctgtttt ttattcatgg accggtccat ttatggactc ggttgtgagt tttatatccg
      241 catacttccc tatatttctt gttggtgctg tttttggaac attaatgaat gcaagtggtg
      301 cagcattaac tattgcccat tttatatcag ataaaatggg tgagaagagg gctattattg
      361 cagttattat tgcatcattt ttattaactt atggaggaat atcagttttt gttgtggttt
      421 ttgcagtttt acctattgcg agaactatat ttataaaaag taattaccct caaagattat
      481 tacccagtgc tatctgtgct ggttgcttac cagcatatgt agctcctggt tctgcccagt
      541 ttataaatgc aataccaatt cctgtgttca atacaaccat atattcaggt ttagtaattg
      601 gtatcgttgg cactgctgta tggttattgt tcgctgtatg gtatatgtct gcaaaaatta
      661 aaaatgctaa gataaaagga gaaggatatg gtacagaagt aagtaatgac tgtaatcttg
      721 agactggaaa agttactttg gcatttatac ctatactgat agttattata ggaaatgcat
      781 ttttaacata ttttttccag attgacgaag tggttgatta ttacagagat tttggaggtg
      841 ttcagggaac atgggcaatt ttaatttcca tagcaattgc tattgtcgtc actatttgta
      901 tttactggaa tacttttaaa agtgatataa agaaaacact tacattaggt gctacagagt
      961 ctcttttacc aatatttaat acatctaccc aagttggtta tggtggggta attaaagtcc
     1021 ttcctgtgtt tacccttatt caaacaacaa ttttttctat tcctggagta gcattatttc
     1081 ctgcaattat aggtacagca attctttcgg gaatagtggg ctcaacatca ggaggggtgg
     1141 gattagttat ggtaacgttt ggtcaagact tattagaact atctcaagct cagaatattt
     1201 cgccaggttt aatgcatcgt attattgttt ttagtgcatc aacgctggat acgcttcctc
     1261 attctggctt tattatcaca ttgctaggag tttgtggctt atctcataag caaagttata
     1321 aagagctttt tattgttact tgtgtgtttc ccgcatttgc ggttctctcc atgttggtat
     1381 atgcattctt atttttaatt taaaagaggt ttaatatgtc tatttattca agaaaacaat
     1441 gtacactaga agatgccgta aatatgattc atgataatga ttgtgttatt cttggcggat
     1501 ttattggtgc agttgttcct gagtgtatag aaaaaggcat agaagatcgt tttttgaaaa
     1561 cgggaaaacc aagaaatctc aagattattt ttgctgctgg tcaaggggat gcaggaacaa
     1621 aagctgtcaa tcatttatct catgaggggc ttgtcactag tgccattggc gggcattggg
     1681 gactaatacc taaattgcag aagttagcta atgagggaaa aattacgggt tataatcttc
     1741 ctcaaggggt tatttgccat ttattccgag attgtgctgc tgggaagtta gggacattaa
     1801 ctcatgttgg tttaggtaca tttgttgatc cccgcgtaga gggcgggaaa attaatgata
     1861 aaacacaaga ggatattgtg tccgtcacag aaattaatgg tgtggaaaac ctattttata
     1921 agaagattga tgctaacata gcaatcctca gaggtactac atctgatcag agtgggaata
     1981 taacaatgga ggatgaatgt ttgattcttg aaaacctagc tgcagcccag ttagtccata
     2041 ataatggagg gaaagttatt gttcaggtaa aacaaatagt acgtgacggt acattagatc
     2101 ctttacaagt cagaattcca ggtatttatg ttgatgctgt tgttgtttct gaagatgaat
     2161 atcatatgca aacttttgcg gagtatatga acttcagcta tgtcgggaaa ggtgtaaaag
     2221 aggtaaaaga caaagaaaca cgccctcttg atataaaaaa aattatagct cgacgagccg
     2281 caatggaact gaaaaaaaat gcagttgtaa attatggcat aggggcacct gaagttatag
     2341 ctgaagtaac agatgaagag gggattacct ccgaacttat cgcaactgtt gaaccaggtg
     2401 caattggagg aacgcctgct ggaggtctgt catttggagc ttcttcgttc cctcaggcga
     2461 taattactca ggatcagatg tttgatttct atgacggtgg tgggcttgat caagcgtttc
     2521 ttggattagc tgaaacagat gaaaaaggag atttaaatgt ttctaaattt ggaacaaaaa
     2581 ttgcagggtg cggaggattt attaacataa ctcaaaatgc taaaaaagta tatttctgcg
     2641 gttctttcac tgctggaaaa aataagattt atgtcagcaa tgggaaattg catataaaag
     2701 aggatgggag tgtaaaaaaa ttcattaaga aggttcaaca aatcactttc tcgtcagata
     2761 ctgcaaggaa aaataataaa gaagtgtttt atattacaga gcgtgctgtt tttagacttt
     2821 ctcaaaaatc cattgagttg attgaaatag cgcccggagt cgatttgcaa aaaaatatta
     2881 ttgaatgcat ggagtttgtt ccagaaattt ctccgtcttt gaaaattatg gatgagcgta
     2941 tttttttgga acaaccgatg ggattagaac taaaggatta atcatggtgt acactattga
     3001 taaactacac ttaggtcaga aggcgagctt tgaaaaaaca ataacagagg cagatgtttt
     3061 aattttttct ggtatttctg gcgatattaa ttctgttcat attaatgagg aagctgcaaa
     3121 gaagagtatt ttcaagaaac gagtagctca tggtgttctt gtatcatctc taacatcagc
     3181 agtattaggt atgaaactcc caggtgaagg cacaatatat cttcgtcagg aaaacaattt
     3241 tcgcaaacct gtttttattg gtgacactgt taaagcaact tgcgaggtag ttgatattaa
     3301 taaagaaaaa aatattgtaa agctttcaac aatcacaaca aatcaaaatg atgaggttgt
     3361 aatcgaaggc attgcagtag tcatggctcc aaaatcttaa tttatttaac tatcaaggac
     3421 cttatatgat tataaaacct gtaattaaag gtgttgtctc gaaaagtgct catccagagg
     3481 gatgtcgtca tgcgataatg gaacagatta agtattgcca gtcccatctt cagcaaatta
     3541 ataatggccc gaaacgagtt ctgattctcg gtgcatcttc aggatttggt cttgctgcaa
     3601 gaatttctct cacttttggc ggcgcaaagt gcgattctat cggagtttcg tttgaaaaag
     3661 gtccctctga gaaatctata ggaactgcag gatggtataa taatatattc tttaaggaat
     3721 ttgctgaagc tgaaggcaga attgctcata atgttattgg cgatgctttc tctgatgatg
     3781 taaaaaataa agttcttaac ttaataaata aggattttga tggcgaggtc gatcttatta
     3841 tatatagtct ggccacaggg caaaggtcag atccagcaac aggtaacatt tggcgttcct
     3901 gtttaaaaac gactggcagt gcttttacag ggaaaacggt taatatcgaa gataataaac
     3961 ttgaatcaac aattattgat gttgcttcag aggctgaaat tgcagatact ataaaagtta
     4021 tgggggggga tgactgggag tcatggatac tttggttaaa ggaaaataat gttcttacta
     4081 agaagaccaa aacaattgct ttttcatata tcggtccaga aatgacttat ccgatttatc
     4141 atacagggac attaggaaaa gccaaacaac atttacacga aacatctgat cgtctaaata
     4201 aattaatttc cacagataaa catagtggcg cttatgtagt agtttgtaaa gctttagtta
     4261 cgaaggctag tgtgtatatt ccctgtttct ctccttatat actctgctta tataaggtaa
     4321 tgaagtctat gggattgcat gaaacatgta ttggacaaat gcatcgtctt tttactgaat
     4381 atttatacaa tgtcgataat gttgttgttg attcatcaag actaattcgc atagacgatc
     4441 ttgaattaag agaggatgtg caacaaaaag taattgattt gatgtcaaaa atgaatgatg
     4501 aaaactttac ttctgtaggt gattatatcg gatacaaaaa aga
//
LOCUS       DAZGCQ010000231         4457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000231 DAZGCQ010000000
VERSION     DAZGCQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.233"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..333)
                     /gene="ymgC"
                     /locus_tag="ACHK07_005112"
     CDS             complement(85..333)
                     /gene="ymgC"
                     /locus_tag="ACHK07_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO8105297.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(446..712)
                     /gene="ariR"
                     /locus_tag="ACHK07_005113"
                     /gene_synonym="ymgB"
     CDS             complement(446..712)
                     /gene="ariR"
                     /locus_tag="ACHK07_005113"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO8105298.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(741..1013)
                     /locus_tag="ACHK07_005114"
     CDS             complement(741..1013)
                     /locus_tag="ACHK07_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO8105299.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSVQTLIKQLLVMKSAE
                     ENAFRQDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
     gene            complement(1056..1292)
                     /gene="ycgZ"
                     /locus_tag="ACHK07_005115"
     CDS             complement(1056..1292)
                     /gene="ycgZ"
                     /locus_tag="ACHK07_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO8105300.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            1606..2817
                     /gene="bluF"
                     /locus_tag="ACHK07_005116"
     CDS             1606..2817
                     /gene="bluF"
                     /locus_tag="ACHK07_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO8105301.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            3012..3743
                     /gene="bluR"
                     /locus_tag="ACHK07_005117"
     CDS             3012..3743
                     /gene="bluR"
                     /locus_tag="ACHK07_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO8105302.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3964..4368
                     /locus_tag="ACHK07_005118"
     CDS             3964..4368
                     /locus_tag="ACHK07_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO8105303.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT         1337 a          915 c          877 g         1328 t
ORIGIN      
        1 ttaagaaagc ataacgcctg aaatgctcac tttgcatcag catggtgata cagctgatgt
       61 ttattctaaa accttactca agttctaaga gagcacggat tccctgtcat taataatgat
      121 atccgttagt gtagtcttat gcatttcaat gtactgcaat atttgcagat agagatcaga
      181 aagaattaca ctggtttcat cttttaaacg caagcgtaaa gcctgatcaa taatatgact
      241 atcaacatac ccatgcgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg
      301 aaaaataaat ctctctggga ttgaattatt catgtcagcc tcgtagaaat attaacccaa
      361 taaaattatg ctgtgtatag tctaaagcga cagacaaaaa taagatgaat gagttttcta
      421 taaacttata cttaataatt aaaagttaca tatcatccgc tgtgtatcgc aacacgattt
      481 ccagtgtttt tctgatcaca tcagctttga gaatatcact tgttgtttcc agggagtgta
      541 tcagacttaa gataatattt ttattattaa cattatcgcc tgagagcatt aaattagtca
      601 cagcctgccc taacactgct gactcttctt ctaacaaatt acccgaactg cgaaagtaac
      661 ttgataacgc tttatcagtt attgcattat gaattgtagt atcttcaagc atggtaacct
      721 ctcatcttac ttatgaaatt ttagtgtatt ctgtttattt tcttaccatt gaacatactt
      781 ttattgtcag ctatcgctgt tcttcggttt ggccctcctg tatcactgtt gctaaaatca
      841 cgaattatgc tgtcaattaa atcttgtcgg aaagcatttt cctcagccga tttcattacg
      901 agtaattgct taataagagt ctgcaccgaa aaatttatct tcatgcgcag tatctggagc
      961 accgcctggc cagtaatttc atattttata ctttcattat cagatgtctt cataacgact
     1021 tcatagctaa cctgttaaat aatcaggctg ttgcattatt caaaaagcaa cccaattagt
     1081 gcattataat gtttctgttc ctcttcccct gtcgcttgtt ccagtcggca aagaagtttg
     1141 gcacaaagcg actttcgact cagattacgt ccatctttca aaatctcagt cacaatttcc
     1201 ccgagagttt cctgctgagt aggcaagtta gcttttgcaa agtaacgagt gattgctccc
     1261 gcagaatcta aagtcactga attttgatgc atgctacgcc tctgttaaaa atgttaatca
     1321 atgttaagtg aggtaacaat agcaaacttg tacgaaatat gtgtacagaa tgataaatta
     1381 attttttgca attagtgcta atgcatataa aacaatatgt tatgataaaa aatattgcat
     1441 catttaactg acaacctata caagattttt cgccaactcg tgttcaactt tttccatttt
     1501 ctatcaatgt aaagaaacag ttatgttttg gtattaaaag aaagacaccg gaagatatgt
     1561 ctgttaccgt cttactctcg cctctcccat taccctggac agttaatgct taccaccctt
     1621 atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg
     1681 atagcaaatc gcaggaacat gcagtctgac gtaacaggga tcttactgtt taatggttct
     1741 cattttttcc agcttctgga aggtccggaa gaacaggtta aaatgatata tcgggctata
     1801 tgccaggatc cacggcacta taatattgtt gagctgctgt gcgattacgc gcctgctcgc
     1861 cgttttggca aagcgggaat ggaattattt gatttgcgcc tgcacgagcg agatgacgtt
     1921 ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga tgacagagcg
     1981 ctacaatttt ttcgtacttt tgtccttgca accgaacaat caacctattt cgagatccct
     2041 gccgaagact cctggctttt tatcgctgac ggatctgata aagaacttga ttcctgtacc
     2101 ctttcaccaa ctataaacga ccactttgcc tttcatccta ttgtcgatcc cttatcgcgg
     2161 cggataatcg cttttgaagc cattgtgcaa aaaaatgaag acagcccgtc agctatagcg
     2221 gttggtcagc gtaaagacgg ggaaatctac acagcggatc ttaaaagtaa ggcgcttgca
     2281 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tatcaatcaa tctattacct
     2341 atgaccctgg ttaacgagcc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
     2401 ggtctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat atctcggttt
     2461 gatgagtttg ccgaagcggt taaatcgcta aaggctgccg gtatcagtgt agcaattgat
     2521 cattttggcg caggttttgc tggtttgtta ctcctgtcac gcttccagcc tgacagaatt
     2581 aaaatcagtc aggaattgat taccaatgtt cataaaagcg gaccacggca ggcaattatt
     2641 caggcgatca tgaaatgctg tacatcactt gaaattcaag tctctgctat gggcgtggca
     2701 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggggatctg
     2761 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagaaaa aaaataattt
     2821 tcagcacata atttcagtaa atcatcgtga aaatgaatac gttatgttta aatttaatcc
     2881 acattaaaaa taatgtttca tccaaattag ctgaaatctt atttatactt tgtacaattt
     2941 tagcgtatat tgtacaaagt attattgggt cgtttacagg cgacggagat ttgtgaccgc
     3001 aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc
     3061 ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag cgcagtgaag
     3121 gcggacaccg actctttgat gaagaagaca tacaacgtat cgaagagatc aagcgttgga
     3181 taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc agccaggata
     3241 cggaagatga ctggagccgc ctgcaagaag agatgatgtc aattcttcgt atggctaatc
     3301 ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta gatcaattga
     3361 ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca
     3421 ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctcg cttttcgaaa
     3481 tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa gagagagctc
     3541 gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcatatttct gttcttgctg
     3601 atcccattga atcaccgcgc ccggaactgt tcccgacgca aacattgatt gtctggacag
     3661 gcatggcacc aacgagaagg caaaacgaac ttttacaaca ctggggtgag caaggttata
     3721 aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca gaatgttata
     3781 atctgaaccc acaacagcgt ttgtaccgga gctggtccat tacaaaatgc tcaccgggag
     3841 aattcaggta atcccccttt cacactttgt acatatcaca gccaaaggac agaccagatt
     3901 gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga ggttattatc
     3961 ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac
     4021 ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat ttattatctg
     4081 gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc
     4141 aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga ggccgcattg
     4201 cttcatattt gcggaaaatc tacatttcgg gaatactgtt caacactggc aggcgcgggc
     4261 gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat
     4321 acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggat
     4381 gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa aagtacagag
     4441 actatcccta tggatga
//
LOCUS       DAZGCQ010000232         4383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000232 DAZGCQ010000000
VERSION     DAZGCQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.234"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK07_005119"
     CDS             <1..207
                     /locus_tag="ACHK07_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8105304.1"
                     /translation="APKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVG
                     SRVVSQEISTADEGDGGQVVVIGR"
     gene            268..3666
                     /locus_tag="ACHK07_005120"
     CDS             268..3666
                     /locus_tag="ACHK07_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8105305.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALF
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASASFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASKLEQFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            3733..4332
                     /locus_tag="ACHK07_005121"
     CDS             3733..4332
                     /locus_tag="ACHK07_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8105306.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT         1037 a         1098 c         1350 g          898 t
ORIGIN      
        1 gcaccgaaag ccaggacgcc cacggcagcc agtacagata acggcaaaca gaacacgtat
       61 ttctcgtcac tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa
      121 atgcgtgtgg ggtctcgtgt ggtttctcag gagatcagca cggcagatga aggggacggt
      181 ggtcaggttg tggtgattgg ccgctgatgc aaaatgtttt atgtgaaacc gcctgcgggc
      241 ggttttgtcg tttatggagc gtgacgaatg ggtaaaggca gcagtaaggg gcataccccg
      301 cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga tgccatcagc
      361 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
      421 gtgctggaca gtgaggggaa taccaatata tccggcgtca cggtggtgtt ccgggccggt
      481 gagcaggagc agacaccgcc ggagggattt gaatcctccg gctccgagac ggtgctcggt
      541 acagaagtga aatatgacac gccgatcacc cggaccatca cgtcggcaaa cattgaccgt
      601 ctgcgtttta ctttcggcgt gcaggcactg gtggaaacca cctcaaaggg ggacaggaat
      661 ccatcggaag tccgcctgct ggttcagata caacgtaacg gtggctgggt gacggaaaaa
      721 gacatcacca ttaagggtaa aaccacttca cagtatctgg cctcggtggt ggtggataac
      781 ctgccgccgc gcccgttcag tatccggatg cgcaggatga cgccggacag caccacagac
      841 cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa ttatcgatgt gaaacagtgc
      901 tacccgaaca cggcactgtt cggcgtgcag gtggactcgg agcagttcgg cagccagcag
      961 gtgagccgta attatcatct gcgcgggcgt attctgcagg tgccgtcgaa ttataacccg
     1021 cagacgcggc aatacagcgg tatctgggac ggaacgttta aaccggcata cagcaacaac
     1081 atggcctggt gtctgtggga tatgctgacc catccgcgct acggcatggg gaaacgtctt
     1141 ggtgcagcgg atgtggataa atgggcgctg tatgtcatcg gccagtactg cgaccagtcg
     1201 gtgccggacg gctttggcgg cacggagccg cgcatcacct gtaatgcgta cctgaccaca
     1261 cagcgcaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta
     1321 tggaacgggc agacgctgac gttcgtgcag gaccgaccat cagataaggt gtggacctat
     1381 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc
     1441 ctgaaggacc gccataatgc cgttgaggtg aactggattg acccgaacaa cggctgggag
     1501 acggcgacag agcttgttga agatacgcag gccattgccc gttacggtcg taacgtcacg
     1561 aagatggatg cctttggctg taccagccgg gggcaggcgc accgcgccgg gctgtggctg
     1621 attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtgggtgc ggaagggctt
     1681 cgccatgtac cgggggatgt cattgaaatc tgcgatgatg actatgcggg tatcagcatc
     1741 ggcgggcgcg tgctggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc
     1801 acgctgccat cctccggtac cacgctgata agcctggttg acggaagtgg caatccggtc
     1861 agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg tgttcctgac
     1921 ggcgttgccg gatacagcgt gtgggggctg aagctgccga cgttgcgcca gcgcctgttc
     1981 cgctgtgtga gtatccgtga gaacgatgac ggcacgtatg ccatcactgc cgtgcagcat
     2041 gtaccggaga aagaagccat cgtggataac ggggcgcact ttgacggcga ccagagcgga
     2101 acggtaaacg gtgtcacgcc gccagcagtg cagcacctga ccgcagaagt caccgcagac
     2161 agcggtgaat accaggtgct ggcgcgctgg gacacgccga aggtggtgaa gggtgtgagc
     2221 tttatgcttc gcctgaccgt ggcagcggat gacggcagtg agcggctggt cagcacggcc
     2281 cggacgacgg aaaccacata ccgcttcacg cagctggcgc tgggaaacta caggctgaca
     2341 gtccgggcgg caaatgcctg gggacagcag ggcgatcctg catcggcatc gttccggatc
     2401 gccgcaccgg cagcgccgtc gcggattgag ctgacgccag gctattttca gataaccgcc
     2461 acgccgcatc ttgccgttta tgatccgacg gtacagtttg agttctggtt ttcggaaaag
     2521 cggattgcgg atatcaggca ggttgaaacc acagcccgct atcttggtac ggcgctgtac
     2581 tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attactttta tatccgcagt
     2641 gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg
     2701 gaaggttacc tggatttttt caaaggccag ataaccgaat cccatctcgg caaggagctg
     2761 ctggaaaaag tcgacctgac ggaggataac gccagcaaac tggagcagtt ttcgaaagag
     2821 tggaaggatg ctaacgataa gtggaatgcc atgtggggcg tcaaaattga gcagaccaaa
     2881 gacggcaaac attatgtcgc gggtattggc ctcagcatgg aggacacgga ggaaggcaaa
     2941 ctgagccagt ttctggttgc cgccaatcgt atcgcgttta ttgacccggc aaacgggaat
     3001 gaaacgccga tgtttgtggc gcagggcaac cagatattca tgaacgatgt gttcctgaag
     3061 cgcctgacgg cccccaccat taccagcggt ggaaatccac cggcattttc cctgacaccg
     3121 gacggaaagc tgaccgctaa aaatgcggat atcagtggca gtgtgaatgc gaactccggg
     3181 acgctcaaca acgtcacgat taatgagaac tgtcagatta aggggaaact gtcagccaac
     3241 cagattgaag gcgatattgt caaaacggtc agcaagtctt tcccccgcac gagcacttat
     3301 gccagcggca ccatcacggt aagaatcagt gatgatcaga aatttgaccg gcaggtcatg
     3361 ataccgccag tgttattccg cggtggtaag catgagaatt tcaacagtaa taaccaacag
     3421 tcatactggt attcaacctg ccggttaaga gtgacccgca atggtcagga gatttttaat
     3481 cagtccacga cggatgctca gggcgtattt tcctcagtta tagatatgcc tgccggacag
     3541 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
     3601 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
     3661 agctgaattt tataacccag aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
     3721 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat ttgtctggcc
     3781 gtatccggtg cgcctgcatg ggcatctgaa catcagtcca cgctgagcgc ggggtatctt
     3841 catgcccgga cgaacgttcc cggcagtgat gatctgaacg ggattaacgt gaaataccgt
     3901 tatgagttta cggacacgct gggaatggtg acgtcattca gctatgcagg agacaagaat
     3961 cgccagctta cccgttacag cgatacccgc tggcatgaag attccgtgcg taaccgctgg
     4021 ttcagcgtga tggcggggcc gtctgtacgc gtgaatgaat ggttcagcgc gtatgcgatg
     4081 gcgggtgtgg cttacagccg tgtgtcgact ttctccgggg attatctccg cgtaactgac
     4141 aacaagggga aaacgcacga tgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
     4201 tctctggcgt ggggggctgg cgtgcagttt aacccgaccg aatccgtggc cattgatgtc
     4261 gcttatgaag gttccggcag tggtgactgg cgcactgacg gtttcatcgt gggtgtcggt
     4321 tataaattct gattagccag gtaacacagc gttatgacag cccgccggtt caggcgggct
     4381 ttt
//
LOCUS       DAZGCQ010000233         4348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000233 DAZGCQ010000000
VERSION     DAZGCQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.235"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            560..817
                     /locus_tag="ACHK07_005122"
     CDS             560..817
                     /locus_tag="ACHK07_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO8105307.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            820..2028
                     /locus_tag="ACHK07_005123"
     CDS             820..2028
                     /locus_tag="ACHK07_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105308.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFAYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASPREEDADALSMVVLSNRNTHAWPDALC
                     LHRPERGTETMLTKTLTRALLWAKTRPNELKGSWISGPTLTSGSSWNNACEENGVTFS
                     LSEDNFSIDPVLGYTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKE
                     EVRKESPGNG"
     gene            2021..>4348
                     /locus_tag="ACHK07_005124"
     CDS             2021..>4348
                     /locus_tag="ACHK07_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO8105309.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASAIGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKTAATGVIFGPEGTVKGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLVVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQAQETAGQAADTTKPLAERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTL
                     AALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHAYDILKSYLMLA
                     RPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDEL
                     ISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVP
                     GMFTRQAWEEQVQDAINEVVRTRRDEIGWVLTDKTHQTGSDISPEALKSRLTERYFTD
                     FGNAWLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEAL
                     ADSVVDSAKKLLDKNKNGRQFIEQTQGPEGPLDGVF"
BASE COUNT         1084 a         1050 c         1195 g         1019 t
ORIGIN      
        1 ggacgggtgt aataatcaat aaatctctca tgaatattca tcataatgag aaaacgacag
       61 tagtaaaatt cggatgcgac atgtatcagt gaacgttcag ggttgagtta atagattcga
      121 ctctaaatcc gtgataaatg ttaatttaag ctaagcaaat ggacttaaga aaaacatggc
      181 ttatttgcat tctcacatgt aataattttg ctgttgtagg tgaaatacca agggtaatga
      241 tttcctcaga aaagaatgtt tgtggtataa aaattaatga cgagtttgta aatatgtagg
      301 tggttacaaa tgtgaatttt attgcgcact atctgtagta tcgcatccat atttgtatga
      361 aattggcaaa aaataatagt atttattgat aacccaatgg taaatgcatg cgatggagaa
      421 ctctatctat caataatcag tgacccggaa acattcataa taaaaatcca gacaatcact
      481 atcgatggca gagcccagca taaacatggg aattaaagag tgatgaatta gttaagagat
      541 aagaaagagg aatttaataa tgcttcatat tattcgtaaa ggcgataaaa ctactcacgg
      601 aggttccgtt ctgaccgctt ctgaaacgat gaaattcggt gggataggcg tcgcccgtaa
      661 aggcgataaa gtgtcatgtc cgggtcatgg tcctaccatc atcatagaag gcaatcctga
      721 ttatcttgac catggtatcc cggtggcatt tcacggtcat aaatgcgcct gtggttgcac
      781 attgatcagt tcatttgccg cagcaaaggt tgcctgatta tgcctgtctg gctggatgcc
      841 atccctgaaa aggccccaaa gatagcgcgc cccaatactc gccgctggct gttgttcctg
      901 gcatttatca tgctgggggg gatggcgctc acattatggt gctggacgtc agaacgaaca
      961 ggatttgtct tctggttcac cgctctgggg cttccatttt gcacgtgggg gcttattttc
     1021 ggtttacgtc ggtttgccta taaagccggg caggtggggg ctgagtcccg aaatgtcgag
     1081 cgggaagcgc ttattgacag tgaaattctg cgtggtcagc gctgtgcctg gatcctcggt
     1141 acttatattc agagcccggc cggaaacaaa gccgatgacc tgcttgaggc aatggaagtc
     1201 gcggctcctg ctattgattt ctcgcaccca agaggatgtg ataaaccggt caggtacgcg
     1261 gcattaccag aataccagac agatttaacg aaggctctga aaggggcggt gaataaactg
     1321 accacgcgtg tagaggggat agttcatccg cttccgcccg cgctaccatg ctggctggtg
     1381 ctggattgtg ataacgatct gtatcccctg attgaagagc agcttaaagc cgacctctca
     1441 ctcaagacgg gcaggatttt tcgcctgatg accggaaaag ggctgggggc gtttgatgcc
     1501 tggctggaca agcgatggga caccccgggc attctggttg tcatcacgct gtcgttacct
     1561 gcctctcccc gggaagaaga tgctgatgct ctctcaatgg tggtattaag caatcgcaac
     1621 acccatgcct ggccggatgc gctttgtctt catcgacccg aaaggggaac agaaacaatg
     1681 ctcacaaaga cacttacccg ggcgcttctc tgggcgaaga cccgacccaa tgagcttaaa
     1741 ggaagctgga tctccggtcc cacattgacg tcgggcagca gctggaataa tgcctgcgaa
     1801 gagaacggag tgacattcag cctttcagaa gacaatttca gtatcgatcc cgtgctgggc
     1861 tataccggcc atgctgctcc atggcttgcc atcacgcttg ctgatgctgc ctttgaacaa
     1921 cgaggtgcgc aggttatcgc cgcccagccc acagctgaca aagacgacgt ctgggttgcc
     1981 gtcatcacta aagaagaagt tcgcaaggaa tcacccggga atggctaagg taaaagttaa
     2041 aacactcatc ggctctgttg tgactgtcgc cttttttctg acggtcatcg tggcatttct
     2101 tttttatgcc tttccggaaa acgtcgcttc ggccatcggc ttcggtccgt atgatgggct
     2161 gcgtatactc acattctgcg ccatactggt tgccacgctg tttttactcg gctggctgac
     2221 ggagaaggct ttcgacttcg ccggtaaatc cgggctgtat ttccagtggg ggcagaaaaa
     2281 ggctgagagt atcactccgg tgacgaacac cattcccggt gaagaagacg acgatgagcc
     2341 ggtttttagc gaagatgctg tttcagaaca tttgcgcttt cgctatgggc gtcgctggca
     2401 gcgtaaggtg cgactcctgc tggtgatggg ggatccggat gtggtccgga aagcagcccc
     2461 gggcctttgc catgacctct ggcaggaagg cgacggctac gtgctgattt atggcggaga
     2521 cgcgcagtca ctcccggacg agatcttcct ttcaaaactg aagcgtctgc gatccgggca
     2581 accggttgat ggcatcattc aggtgatgag catttcagcc ctcccgacgg atggcgaacg
     2641 ggatgccctc ctgcgttacc gccagaaagc cgaccatcag ctgggctggc aggcaccggt
     2701 ctggctgtgg ctgactgaca acgctaccgg tgcccggcag gacgcgaaaa cggccgcaac
     2761 aggggtcatc ttcggaccgg aaggaaccgt gaaaggtgcg gacgaagcgt tttccacact
     2821 ggcgttacac ctgcaaaaag caggaatggc gcagatactg aatgaacctg ctgatttcgg
     2881 tctccttcag ttgtcactca gactgcggca ggagctgaaa gccagcctgg ttgttttgct
     2941 cagtggactg atgcagggga ctgctgcctg gcgtttacgc ggtgtcatgt tcagtcctga
     3001 actgaccgtt gccggagcgg taccaaacac atgccccgac acacctgcct ggaaagctat
     3061 tattgatgac tgtgatgccg tcagcggcag gaagctgggc tttaactggc tgaagatgct
     3121 gcgattgatt ctgctttccc tgattgtcct gtggggggcc ggtacactgc tgtctctgct
     3181 cgttaaccgt actcagattt atcaggcgca ggaaacggcg ggacaggcag cggatacaac
     3241 aaaaccactg gctgaacgcc tgcgcagcca gcttgtactc cagcaggcca ttgcccgtct
     3301 gcaacatcgt gaggcaaccg gtgcgccatg gtacacacga tttggtctta atcaggacag
     3361 cgacacgctg gccgcgctct ggccgctgta tgcgaaaaat aacgcacagc tgatgaggga
     3421 tgccacagca gacagcctca ggcagcagct gaatgccttt gtgcaactcc ctcctgccag
     3481 tgatgcccgt acgcagggca cacagcatgc ttacgacata ctcaaaagtt atctgatgct
     3541 ggcgcgcccg gacaaagcgg acgccggctg gctggcaaaa aatgcgctga aagccttacc
     3601 gaaacgccgg aatgtgccgg atggcatatg gcagaacgtc gcaccgaaac tgctggggtt
     3661 ttatgcgcaa aaccttccgg ctcatccgga atggaaaatt aagcctgacg atgaattaat
     3721 cagtacggtc cgtcagattc tgcttaagca aattggccag cggaatgctg agtccgggtt
     3781 atatcaggag atgcttaagc gcgtggccag taactggcct gatttaacac ttgcggatat
     3841 gaccggtgac accgatgcct caacggtatt cagcactgac gaagtcgttc cgggaatgtt
     3901 tacgcgtcag gcctgggaag agcaggtaca ggatgcgatt aacgaagttg tcagaacacg
     3961 ccgcgatgaa attggctggg tactgacgga taaaacacat cagacaggga gcgatatctc
     4021 tccggaggcc ctgaagagcc gcctgactga acgctatttt accgattttg gtaatgcctg
     4081 gctgaacatg ctcaacagca tacagtggca tgaacccacg tcactttccg gagccattgc
     4141 ccagttgagt ctgctcggtg acgtccgcca gtctccgtta gttgcactga tgaacacgct
     4201 gaactaccag gggaaaacgg ggcagaaggg ggaggcactg gctgattcag tcgtggattc
     4261 agcgaaaaag ctgctcgata agaataaaaa tggcaggcag tttattgaac agacacaggg
     4321 ccctgaaggt ccgctcgatg gggtattt
//
LOCUS       DAZGCQ010000234         4325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000234 DAZGCQ010000000
VERSION     DAZGCQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.236"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            145..1242
                     /locus_tag="ACHK07_005125"
     CDS             145..1242
                     /locus_tag="ACHK07_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8105310.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1432..1815)
                     /gene="quuD"
                     /locus_tag="ACHK07_005126"
     CDS             complement(1432..1815)
                     /gene="quuD"
                     /locus_tag="ACHK07_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO8105311.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDVVVNNSN"
     gene            complement(1901..2041)
                     /gene="ylcG"
                     /locus_tag="ACHK07_005127"
     CDS             complement(1901..2041)
                     /gene="ylcG"
                     /locus_tag="ACHK07_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO8105312.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGYVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2038..2400)
                     /gene="rusA"
                     /locus_tag="ACHK07_005128"
     CDS             complement(2038..2400)
                     /gene="rusA"
                     /locus_tag="ACHK07_005128"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO8105313.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2397..2687)
                     /gene="ybcO"
                     /locus_tag="ACHK07_005129"
     CDS             complement(2397..2687)
                     /gene="ybcO"
                     /locus_tag="ACHK07_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO8105314.1"
                     /translation="MVDLRKAARGRECQVRIPGICNGNPETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2680..2850)
                     /gene="ninE"
                     /locus_tag="ACHK07_005130"
     CDS             complement(2680..2850)
                     /gene="ninE"
                     /locus_tag="ACHK07_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO8105315.1"
                     /translation="MTTPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2850..3305)
                     /gene="ybcN"
                     /locus_tag="ACHK07_005131"
     CDS             complement(2850..3305)
                     /gene="ybcN"
                     /locus_tag="ACHK07_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO8105316.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3302..3400)
                     /locus_tag="ACHK07_005132"
     CDS             complement(3302..3400)
                     /locus_tag="ACHK07_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105317.1"
                     /translation="MRPQVELLALPQMQKCTCVFLHPTFDLCGGEA"
     gene            complement(3476..3727)
                     /locus_tag="ACHK07_005133"
     CDS             complement(3476..3727)
                     /locus_tag="ACHK07_005133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105318.1"
                     /translation="MMSFTKCKLMHSHKTSVNAALLDDTKLVVERDLHFLRELYISSP
                     EEFVGHWVYANEPIFHEHPLSVGLSGIVYHKVIDEIEKS"
     gene            complement(3787..4116)
                     /locus_tag="ACHK07_005134"
     CDS             complement(3787..4116)
                     /locus_tag="ACHK07_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO8105319.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPRILNRARAILVRETAHVKLQPWQDDKWSRVLPHLR
                     QNVIKQR"
     gene            complement(4228..>4325)
                     /locus_tag="ACHK07_005135"
     CDS             complement(4228..>4325)
                     /locus_tag="ACHK07_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105320.1"
                     /translation="IVFKHYPLMKTISAQQAISTLKYMVDGEVYF"
BASE COUNT         1176 a         1011 c          931 g         1207 t
ORIGIN      
        1 aaagttcaga taaaaataga gatctacttc acaaattaaa tgagaaacta aaacttacat
       61 cttgaaataa tcacattgat tagatgaata tttatctcgc agtgacatca ttttttaata
      121 atagttcaaa aaaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct
      181 gcatcagtac tgatggcgat gtctgctcag gcagctgaaa tttataataa agacagtaac
      241 aagctggatc tgtacgggaa agttaatgcc aagcactact tttcctctaa cgatgcagat
      301 gatggtgata ctacttatgt tcgtctgggc ttcaaaggcg aaacccaaat caacgatcag
      361 ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca accgcgctga atctcaaggt
      421 tcctccaaag acaaaacccg tcttgcattt gcaggcctga aattcggtga ctacggctca
      481 atcgattacg gccgtaacta cggtgtagca tacgacatcg gtgcgtggac tgacgttctg
      541 ccagaattcg gtggcgatac ctggacccaa acagatgtgt tcatgactgg tcgcaccact
      601 ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg ttgatggtct gaactttgct
      661 gctcagtacc aaggcaaaaa cgatcgtagc gatttcgata actacaccga aggtaacggt
      721 gatggcttcg gtttctctgc tacctatgaa tacgaagggt tcggtatcgg tgcaacttat
      781 gcgaaatctg atcgtaccga cactcaagtt aatgcaggga aagttcttcc tgaagtattt
      841 gcttccggta aaaatgcaga agtttgggcc gcaggtctga aatatgacgc taacaacatt
      901 tacctggcca ctacctattc tgaaacccag aatatgactg tatttgctga tcacttcgtt
      961 gctaataaag ctcaaaactt cgaagctgtt gcacaatatc agttcgattt cggtctgcgt
     1021 ccgtccgttg cttacctgca atctaaaggt aaggatcttg gagtatgggg cgatcaggac
     1081 ttagtcaaat atgttgatgt aggtgcaacc tattacttca acaaaaatat gtctactttc
     1141 gttgattaca aaatcaacct gcttgacaaa aatgacttca ctaaagcact cggtgtaagc
     1201 actgatgaca tcgttgctgt aggtctggtt taccagttct aatctgatta cgaaaaagat
     1261 atgttgcggg aggctttgcc tccccaacat ataagtggct ccctcaagcc acttccttta
     1321 ggagcacaac cttgcttcta actatataaa ccttctgtta tatattaccc tttatttttg
     1381 ggggcgttgc aacgccccat ttttaataat ttttagtaaa caattggcat attaattaga
     1441 gttattaaca acgacatcca tctctaaccg gatatctaat gccattaaca tcccttcaat
     1501 tatgccctca gccttctgta accttttccc gatataacca tcagagcagc aatgcttacc
     1561 tgccagtgac atgaatgtca taccgactac ataataatct actaataaat cgtgcaaatc
     1621 gctgttgttc tttttcagac gggccatgca cccgcaaatg atcatcgcgt catcgtcaca
     1681 acattgcggg cgagatttta cttttgaagg aattaatccc ttaaaaccgg cggcaatgga
     1741 cgaccaggtc acatcttcat gattattagc cgcccacgct ccccaacgct caagaaccat
     1801 ctgaatatca cgcatcaact tactccacaa aactcagacc agaacgccaa ttacaagcaa
     1861 aaatcaacaa aacagtatta gttgattgtt atctctgact tcatactcct gctcctgtca
     1921 gtgttttggc gtaattcttc agtattcggt aatcggtcaa aacataaccg gggaaacgat
     1981 ataagcgcag acgcccccag cggtggcgaa gaagttctgc catattaaac tcaaacatca
     2041 ttcattcccc atttcggtga tggtcagttc cagcctccca cctttggtaa caggcatctt
     2101 cacaacgcgg taatcaacga cctgagcatc atccagccag aaacctgctt tagtgagtgc
     2161 gtcaaaagcg gctttttgca gattatccag gtcacggcga cggcgatccg gcatgtggca
     2221 ctcaatgcgg attttcacag gcatagccag gccgatatcc agcattgcgt ttttaatgat
     2281 tcgggcgacg ttatcgcggt atgcctgccc ctctgcgctg atgtgcgtgc gcccgcgatt
     2341 atggcggtaa tagcgattat tgctcggagg ccagggtaat gtgatgctgt aggtattcac
     2401 gccttaataa ccccctcttt cagccagata acctgtgttc tcgccatacc ttccagcgcg
     2461 cattcttttg catatccagc gtcaacaaaa tgcgtgcgac ggtcgatctc atcgtgacag
     2521 gcagaacatg caatggtggc aatcaggtct ggcggtttgg taccggtgcc acacaatcca
     2581 gccagccgga tatgtgccag tacagacgtt tcagggttgc cattacatat gccagggatt
     2641 cttacctggc attcccgacc acgcgctgct tttctcaaat caaccatgat tcctccttgc
     2701 tgccagtcgc aaccattttt tatcaaccag gctggcggta taccctagca gtgttggtat
     2761 ttcggatggc ttcagctcag gcttacgctt acgacgattt ggtactctgt agatgtgtcc
     2821 gttcatgaca cgaataagcg gtgtagtcat tacgcctcct gcttgtcgcg cagcagctgg
     2881 aactcgcagc tctgtggaat agtcaggtgg caaccaatat tcatcgccca ggcttcaacc
     2941 ttacacagga agacatacat ctctccggta tcaagatcgg aggtatggcg taacgactgg
     3001 atcgtagtga tttcgccggt tacgacatca accaggtcct tggtttcata accgaggtat
     3061 gtgtgtttga gagcatcttt tacccatgct gcagtagcga acgatttccc cctgctgatg
     3121 aggtattcac tgatttcgct gtaccacatg tggctgagtg cattctggga aagactgcgt
     3181 ctttcgcgcc acggtttaag caccatgcga aagcatttgc cgtcctccag ataaggctgg
     3241 atctgctgac cgatagcggt gaagttaccg cgatgcaatt tgataccgtc ttgtgggagg
     3301 ttcacgcttc acctccgcag aggtcaaacg ttggatgcaa aaaaacgcag gtgcatttct
     3361 gcatctgtgg caaggcgagg agttcaactt gtggtcgcat ataatttccc aatcatatgc
     3421 gcagaagtca ccgcagggtg ttcaggccaa cggtgacggt attattgctt attgattatg
     3481 atttttcaat ttcatcaata accttgtgat agacaatacc agaaaggcca acagacagtg
     3541 gatgttcatg aaatattggc tcatttgcat aaacccaatg cccgacaaat tcttctgggc
     3601 ttgatatgta taactcacga agaaaatgga gatccctctc tacaactaat ttcgtatcgt
     3661 ccaagagcgc tgcatttacg ctagttttat ggctgtgcat taatttacat ttggtgaagg
     3721 acatcatggc aaaagctcct ttttcttatg aatccatatg ttagctaaat tggagagaca
     3781 cagtagctaa cgttgtttaa taacattctg acgcaaatgt ggcaatactc gactccactt
     3841 atcatcctgc cacggctgaa gttttacatg tgccgtttct cggacgagga ttgctcgcgc
     3901 tctgttgagt atccggggat attcttgctc gatggaagtg aagtgaccag cttcaccatg
     3961 ctccgcatcc tgaagaagat gagtaacgtt ctggtaggca attaacatca cattccctgc
     4021 tcgccataac caggcgagtg tgcaaagttc gttatcagtg aattgttttg tgattgggga
     4081 ttgttgaact gctagaacga gaacgccagc atccattggc agtccctata gtaaaaccat
     4141 agctcaggac gcttcgttca ggatagataa ttttattgta cttacctaac tttcttacta
     4201 tagcacggtt gaaaaagtga ttattactca aaaataaacc tcaccatcaa ccatatattt
     4261 gagagtactt atcgcctgct gggcagatat tgttttcatt aaaggatagt gtttaaaaac
     4321 aatgc
//
LOCUS       DAZGCQ010000235         4163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000235 DAZGCQ010000000
VERSION     DAZGCQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.237"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..835
                     /locus_tag="ACHK07_005136"
     CDS             227..835
                     /locus_tag="ACHK07_005136"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO8105321.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHK07_005137"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHK07_005137"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO8105322.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4163
                     /gene="selB"
                     /locus_tag="ACHK07_005138"
     CDS             2321..>4163
                     /gene="selB"
                     /locus_tag="ACHK07_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO8105323.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          933 a         1045 c         1234 g          951 t
ORIGIN      
        1 gctcatcctt gtattatcgg gttatttgtc cgctgacagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat tgggaggtga caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcattac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaat ggcgtggcgc aatttaaccc
      361 gttagggaaa gtgccagtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgccgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccttt
      481 ggagtcgttg cgggtgcgca aaattgaggc gctggccgac ggcattatgg atgctgggct
      541 ggtgtcggtg cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 acttaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgc tgcgtcagat gcttgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt gataaacctg acgggaaccg
     1201 tgctgcatac caatcttggg cgagctttac aggcggaagc tgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gcattaccgg cgcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtttc tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcaca ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag acgaagctga actggtggcg ctcggcaaag
     1681 agctggatgt tccagtggtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 cccttgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgccgatcg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgtgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg
     2821 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 atggctgact ggtgtaaata aaccgatgcg cgtgcgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gctcatgccg ggcagcgtat cgcgcttaac atcgcggggg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacgcg
     3121 ggtgattgtc gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgttgc gcgatatctc
     3301 ggcccgcaat acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggagc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag ccgggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag cggaaaattc ttgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctggtcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atccacagcc atcacggctg gttgcatctg ccagatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gacctggcaa aagagacggg aaccgacgag caggcaatgc gtctgacgct
     3901 acgccaggcg gcccaacagg ggataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgagtttg ccaatatgat ccgcgatctc gatcaggaat gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg ggttgggcga aaactggcga ttcaaatcct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcattatta tttccggaaa aat
//
LOCUS       DAZGCQ010000236         3951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000236 DAZGCQ010000000
VERSION     DAZGCQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.239"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..181
                     /gene="tnpB"
                     /locus_tag="ACHK07_005139"
     CDS             <1..181
                     /gene="tnpB"
                     /locus_tag="ACHK07_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8105324.1"
                     /translation="LWADRDGLCLFSKRLEWGRFVWPVTREGKVHLTPAQLSMLLEGI
                     AWQHPKRTERPGIRI"
     gene            201..1757
                     /locus_tag="ACHK07_005140"
     CDS             201..1757
                     /locus_tag="ACHK07_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO8105325.1"
                     /translation="MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALV
                     AKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRPGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPPGESISEQLDIINIAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMGRWVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYV
                     RDDRNAGSTMPAAVWFSYSPERKGIHPQQHLADYRGILQADAYAGYNALYESGQVTEA
                     ACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPL
                     MQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVV
                     ALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANRVKEL
                     LPWNVTLSVN"
     gene            complement(1975..>3951)
                     /gene="iha"
                     /locus_tag="ACHK07_005141"
     CDS             complement(1975..>3951)
                     /gene="iha"
                     /locus_tag="ACHK07_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO8105326.1"
                     /translation="LTNAAASVSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTG
                     GLEISIRGMPASYTLILIDGVRQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPM
                     STLYGSDAMGGVVNIITKKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDS
                     VSLQIRGSTQQRQGSSVTSLSDTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMD
                     TTRQRYDNQDGQLGSLTGGYDHTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGR
                     ELVRSVLKSDKWGLAGKPRELKESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVV
                     LASTGETFRQKSWSVFAEDEWHLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDA
                     WTLKGGVTTGYKAPTMGQLHKGISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLS
                     GLNANITGFITDFSNKIVSYSIDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLN
                     YTWTQSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYS
                     SLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAG
                     KSSLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT          947 a         1103 c         1001 g          900 t
ORIGIN      
        1 actgtgggcc gatcgtgacg ggttatgcct gttctccaaa cgcctggaat ggggccgctt
       61 cgtctggccg gtaacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct
      121 actggagggg atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata
      181 acccgtgata aaacagggga atgaacaaca cactccccga cgacatcgag caactgaagg
      241 ccctgctgat cgcacagcag gcggttatcg tccgtctgtc tggtgaaata accggctatg
      301 cccgcgagat cagctcgctc agagcgctgg tcgctaaact gcagagaatg ttgttcggtc
      361 gcagcagcga gaaaagccgc gagaagatag aaaagaagat cgcacgggca gaaacgcgta
      421 taaccgagct ccagaacagg cctggtgagg cgcagttgca actcacctca atggccggag
      481 agacagcgcc gaaaacatca gactctcccg tccgcaaagc atttccggca acacttcccc
      541 gtgacaggca ggttatctcc ccggcagaaa ccgaatgccc cgtctgcagc ggcaaactga
      601 aaccgccggg agaaagcatc tctgaacaac tggatatcat caacatcgcg ttcagggtaa
      661 tcgaaacggt tcgcccaaaa ctggcctgca gccggtgcga ctgtatagtt caggctccgc
      721 agccaccaaa acccatcgag cgcagttacg ccagtccggc tctgctggcc cgcataatca
      781 tggcgaagtt cgccgagcat ctgccgctgt accgtcagtc ggaaatctat gcccgccagg
      841 gcgtggagct gcaccgcaat acgatggggc gctgggttga catcatggga gagcagcttc
      901 gcccgctgta tgatgaactg aagcactatg tgctgatgcc gggtaaagtg catgccgatg
      961 acacgccggt aaatgtactg gagccgggtc agggtaaaac ccgtaccgga cggctgtggg
     1021 tctatgttcg tgacgatcgc aacgccggtt cgaccatgcc ggcagcggtg tggttctcat
     1081 actctcccga gcgcaaaggt atccacccgc agcaacatct ggcggactac agaggtatcc
     1141 tgcaggccga tgcatatgcg ggttacaatg ctctttacga aagcggtcag gtaaccgaag
     1201 cggcttgtat ggcacatgcc cgacgcaaga tccacgatgt acatgtccgc catccaacga
     1261 cagtaacggg agaagcactc cgtcgtatcg gggcactgta cgctatcgag tctgagatcc
     1321 gcggcagtcc ggcagaagag cgactggcgg tcagaaaagc cagaacggta ccgctaatgc
     1381 agtcgttgta tgagtggctc caggggcaga tgagcacgct gtcgcgtcac tctgatacgg
     1441 cgaaagcgtt cacctatctg ctgaagcaat gggacgccct gaacgaatac tgccgcaatg
     1501 gctgggtgga gatcgacaat aatctgtgtg aaaacgccct ccgggtagtt gcactggggc
     1561 ggcgtaacta catgttcttc ggctctgatg gaggaggcga gagtgcggca gtgatgtaca
     1621 gcctgatcgg tagctgcaaa cttaacggaa tcgagccgga aacgtggcta cgccacgtga
     1681 tcagtgtcat caacacctgg ccagccaacc gcgtgaaaga gttgttgccc tggaacgtca
     1741 ctctctctgt aaactaattt ttaccccacg tccttcacgg tgcgcttaca ctctggacaa
     1801 gtatgtaaat gataagttaa aaataatccg tgcatttcct gtgacgatat tagttgttgg
     1861 taacgaaatc tcattagcgg atcggaaatg cacttaacct aatacgttgc cactgttccg
     1921 ccaggagttg cagtggcaac gtattctacc gtcagtgata gcgttttgtt attatcagaa
     1981 ctgatagttc agcgacatcc agtaatttcg ctcaggtatc acatatcctg ttgttgatga
     2041 tcccgtctgg aagtaatcac cggcatacag cgaactctta ccggcactgt acaggctcac
     2101 gtcactgtaa tccttgttga gcaggttatt caccgcagca ttcagcgtca gggcatccgt
     2161 catcttccac gacaaacctg catccaccac cgtccaggct ttcaggtatt ctcctttctc
     2221 atcatacact ttcttctgta cagcgctcag tgacgaataa ttccgggtga aacgtggtgt
     2281 tttcccgcga taacgggcac ccagccatga tgccacctct tcggtgatct gccagttcag
     2341 tttcgcattc accatgtgtt caggggtata actcagcggt gcacctttgt tatcaccatc
     2401 acgttgttca ctttgggtcc aggtgtaatt caacgacaga gtgacatcct ctgaccacag
     2461 cggcaatgtg ccggcgaatt ccacaccatg caagcgggcc tttccgctgt ttgtatagct
     2521 attggtgtta tcatcaatgg aataagagac aatcttgttg gagaagtctg ttataaagcc
     2581 tgttatattg gcattcagac cggagaggtt atcgtaatat acccccgcct cataacttac
     2641 actctcttcc ggcttcagtt cggggttacc gagtagatta gtacgtccct gcccggatac
     2701 accgctgatc cccttatgta actgtcccat ggtgggggct ttatatcccg tggtcacacc
     2761 gcctttcagc gtccaggcat cggtcacgtc ccagaccaga tatgcacgcg gactgaagtg
     2821 tcccccgaat tgatcatgat gttcatagcg actgcctgca gtcagcgcaa gtacatccgt
     2881 gagatgccac tcatcctcag caaatactga ccagcttttc tgtcggaaag tttcacctgt
     2941 gctggcaagg acaaccccat ctttcatgga cgaactctga tactctcccc ccacagtaag
     3001 cagatgagat tctcccagag gggtaagcag caatgaattc aggataaggt tagattcctt
     3061 aagctcccgc ggtttaccgg caagccccca tttatcgctc ttcagaacac tacgtacaag
     3121 ctcacgacct ttattttccg tctcgttcca gttcagatac gatttccagg taccgaaggt
     3181 gaaagtatga tcatagccag ccgaaatttt gtttcgctca tagcgcagag tatggtcata
     3241 tccccctgtc aggctaccca gttgaccatc ctggttatcg taacgctgtc gggtggtatc
     3301 catatcaaac cagagcacat cctgctccgt agccttccag tcaatacgcg caccaagatt
     3361 ataattctgt gactccgtgg gataaggaat acgcgtggct gatgtgtcgc tcagtgatgt
     3421 gaccgatgag ccctgacgct gctgtgtgct gccgcggatc tgcagactga cagaatcatc
     3481 cacaagaggg ccactgctcc agaaattaaa ctggctgctg ttgccccatt tgttactctc
     3541 ctgcagattc agcccggcgt tgacggaaga gagccatttg tctgcattct ttttggtaat
     3601 gatattcacc acaccaccca tcgcatcaga gccatacagt gtggacatcg gccccctgat
     3661 aacctcaata cgctcaatcg cggccagagg aggcatgaac cctgtattca tggcagaaaa
     3721 accgttaggc gtcacgtccc cgctcccgcc ctgacggaca ccatcaatca gtatcagcgt
     3781 gtaactggcg ggcattccac ggatgctgat ttccagccca ccggttttac ccgtaccact
     3841 ttcaacatcc acaccctcca cagctctcag tgcctccgcc agatcgtggt actggctgga
     3901 ctgcaattcc tcctgactga ttacggaaac actggcggct gcgttggtca g
//
LOCUS       DAZGCQ010000237         3929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000237 DAZGCQ010000000
VERSION     DAZGCQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.240"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(298..864)
                     /locus_tag="ACHK07_005142"
                     /pseudo
     CDS             complement(298..864)
                     /locus_tag="ACHK07_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
     gene            929..1597
                     /locus_tag="ACHK07_005143"
                     /pseudo
     CDS             929..1597
                     /locus_tag="ACHK07_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
     gene            2012..2893
                     /locus_tag="ACHK07_005144"
     CDS             2012..2893
                     /locus_tag="ACHK07_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbaSI family restriction endonuclease"
                     /protein_id="HFO8105327.1"
                     /translation="MDKTDYILRSLTKTSKKRWEHYAVTRIYHLLNDPDIEFVCQQCV
                     RKENGKIYLADLFFPQLELYLEIDEGHHNSEQSQIDDAKRRMDITEVTGFLEKRIPAS
                     EISLEIFDRYINEFIELIRNRKNRMIKDKKFKEWDYDNKYTARPHLKAGRISVGPDAV
                     FRYHRDALNCFGYNKGHHQSGGWSIPSHISEAMGLSGKCMVWFPKLYGNSDWGNELTD
                     DGNKIIERNIKDDEGYTERWDKRIVMAHSRDELNRTLYRFLGVFEAIPGHHTGNILEF
                     HRVSTSVNVYPPNDAAP"
     gene            complement(3204..>3929)
                     /locus_tag="ACHK07_005145"
     CDS             complement(3204..>3929)
                     /locus_tag="ACHK07_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8105328.1"
                     /translation="QTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSV
                     GSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIETG
                     MRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGATLSYNMRLGN
                     GMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLG
                     VGYSNGADVESPWNAVAGVNWSF"
BASE COUNT         1095 a          975 c          899 g          960 t
ORIGIN      
        1 tacaaacgca ccattctttt cgtcccagta ttagtgaaac tagctacgta ctgcatacct
       61 taagtttatg tgacatacaa aaaataaaac acccactccg cgcgcaatgc tctccccgcc
      121 acgcctgccc gcttaagagg tcgcttttaa tgcaggtgca tgaaccggct caggccgcgc
      181 cggtactggc gcggtgagga aaataaaata cggggatttg catgaaaaac catgcacctt
      241 atggatgcat ggcttttttc ggaaaaaata gcggaatttt gcggattttt ttgcgtgcta
      301 ccgtgcggcc agttctgcac gtcggcgggt gtaaatcagg ttctgtgccg gggtgaattt
      361 ctggcgatta tcatcccgcg aggccgcatc aggcctgaat ccgatggccg ttaaaatatc
      421 gctgtcctgc gcggaataat taattttttc acctgcagcc agccataccg acagggcttc
      481 acgcagataa tcaaccgagt gctgcatggc gctctgtttt actgcgggaa gctgttcgtt
      541 ataccccatc agctcaggtg ccagcgtggc ggccagctcc gcgccgtgct actgcataaa
      601 gtcatggagc cggttgcgga tgctgatgtg ctgcacttcc tcatgtgagc ggatataacg
      661 accggcggcc tgattaatct cccatttttt cacgtcgata ttgtcgcgca aatcctgcat
      721 tctgcgcggg atttgttcgt caccggcaag gagctgttca cggtattccc gttcaaggtc
      781 tgccagttcg gctttacgtt tcagccaggt atttttgttc gtctgacagg cctcaaaggc
      841 ctgctgtatg gtcacagtgg tcacgagtct ctctcctgat taatgccgga acggcgagct
      901 gtagcagccc tgtactttac gcggcggcgg tgacggcgca gggcttaagg ccatcaccgg
      961 cggcgatgcg gtctcggttg acccgaaata ccagaacccg tactcaacac acattccggc
     1021 ggtgattctg gcagtgaaca ataaccccat gcgcttcacc gaccgcagcg gtggtgtgtc
     1081 acgtcgccgg gtgattattc acttcccgga gcaaattgcg ccggaggaac gcgacccgca
     1141 gctcagggat aaaattgcgc gcgagctggc cgtgattgtg cgccagctca tgcagaagtt
     1201 cagcgacccg atggcagcct gcgcactgct ccagtcacag cagaattccg acgaggcgct
     1261 cagcatcaag cgcgatgctg accctacgtt tgatttttgc ggctatctgg aaatgctccc
     1321 gcagaccaac gggatgttta tggggaatgc cagcatcatc ccgcgtaatt atcgtaaata
     1381 tctctatcac gcgtatctgg cctatatgga ggctaacggg tataggaacg tactcagcct
     1441 gaaaatgttc gggctggggc tgcccatgat gctgaaagag tacggcctga attatgagaa
     1501 gcggcacact aagcagggga tacaaaccaa tctgtcgctg aaagaggaaa gctacggcga
     1561 ctggctaccg aaatgcgacg aacccgtagc aacataacct cactcagacc ggcaacagcc
     1621 ggtcttttcc tttctggcca ttgccacaag gtgaacaatc cactgttcac ccttcaccgt
     1681 atcttcaccc tgtatcacca tgaaattatt aataaaaaac cagaggtgaa cagtgtgaac
     1741 agtaaaacct aaaaaaattt tttacccccc ccaccacatc gcctgaccgg gcagatccag
     1801 aacgagcaaa aatcacaaag gggaagattc gacttttcac tcctcaccaa ctcaccaccg
     1861 cttaaccaca tgatataaaa tagaaaatca tcgaagttaa cagtataaac agttagatta
     1921 aaaaaatact aacgcatctt atggcgatag gtaacactat cgccataaga aaaagcacat
     1981 tacatgaaga acaaagaagg aaggcatata aatggataaa actgattaca tcctacgttc
     2041 acttacgaaa acctcaaaaa aacgatggga acattatgca gtaactcgca tttatcatct
     2101 tttaaatgat cccgacattg aatttgtctg ccagcaatgt gtcagaaaag aaaatggaaa
     2161 aatctatttg gcagatttat ttttcccaca actggaactt tatcttgaaa ttgatgaagg
     2221 gcatcacaac agtgagcaat cacaaataga tgatgcgaaa cgtcgtatgg atattactga
     2281 agttacagga tttttagaga aaagaattcc cgccagcgaa ataagtcttg aaattttcga
     2341 tcgttatatc aatgaattta ttgagttgat acgcaatcgt aaaaatagaa tgattaaaga
     2401 taaaaaattc aaagaatggg attatgataa taaatataca gctcgtcctc atcttaaggc
     2461 aggaaggata tctgtaggtc cagatgccgt cttcagatac caccgggatg ctttgaactg
     2521 tttcggttac aacaaaggac atcatcagtc tggcggttgg agcattcctt ctcacataag
     2581 tgaagcaatg ggtctatcag gaaaatgcat ggtttggttt ccaaaactat atggaaattc
     2641 agattgggga aatgaattga ctgatgatgg taacaaaatc atagagcgga atataaagga
     2701 tgatgaggga tacacagaac gctgggataa acgaattgtt atggcacatt ctcgtgatga
     2761 gttgaacagg acactgtacc gatttcttgg ggtgtttgaa gctatccctg gtcaccatac
     2821 tggcaacata ctagagtttc atcgtgtaag tacatcggtc aatgtatacc ctcctaatga
     2881 tgctgcccca tgattaacag catatttagt tccctgttta tacgagatac aacactgcta
     2941 cagtgagaaa attggtacac gtttaggtac acaactgaaa gttgaatacg aaaaaatctc
     3001 ttacttttaa taggttggga aatttattca gactccgcca gccctcatga ttggacggtg
     3061 taaggacaac accaacaaaa acaggaagtt agaagtctca gcaggacacc gaccagacgg
     3121 tgaggagaca taaaaggata cgcaaaggag ccgcggctcc tggtgacatg aaagcccaca
     3181 gatgtgggct ttttcgttga tggtcagaac gaccagttca caccagccac cgcgttccac
     3241 ggggattcca catcggcacc gttgctatac cccaccccga gatgcccgct taacgtactg
     3301 ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgacg
     3361 aaattaccgt cattattcac cttcacccgg ttatcatcga caaattcttt gcgcacagcc
     3421 gccttcagcc acggctcaac ttccataccg ttccccagac gcatgttgta actcagcgtt
     3481 gcccccagtt cacgatatat actgcgggta tcgactgatt tcgatttcat gccattggat
     3541 aaatgatatt cggggttatc agcggtgaac cccgttaacg atgcatacgg cgtcaggttc
     3601 cagttaccat cggtaaatcg catcccggtt tcaatgtgac cgcccagccc gttgctgtgg
     3661 taactgccat tggcggctcc accgctgctc attttaccgg ctacgttact ttcaaaacgg
     3721 ttcagcttca cgacaccgtc cagatagaaa ccactttcat gttcccaact ggcatagccg
     3781 cccagagaat aactgcccac actgccatgt cctccgcgat caaaaccgat atgtgaatgg
     3841 gaataaccca taaaagcgcc cagcgtggca attccttcag gaatatcatt acggctgtcg
     3901 atccccactg tcattccggt cagcgtctg
//
LOCUS       DAZGCQ010000238         3775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000238 DAZGCQ010000000
VERSION     DAZGCQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.241"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..91
                     /locus_tag="ACHK07_005146"
     CDS             <1..91
                     /locus_tag="ACHK07_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8105329.1"
                     /translation="WRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            117..362
                     /locus_tag="ACHK07_005147"
     CDS             117..362
                     /locus_tag="ACHK07_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8105330.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            439..654
                     /locus_tag="ACHK07_005148"
     CDS             439..654
                     /locus_tag="ACHK07_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8105331.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            658..885
                     /locus_tag="ACHK07_005149"
                     /pseudo
     CDS             658..885
                     /locus_tag="ACHK07_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1538..1825
                     /locus_tag="ACHK07_005150"
     CDS             1538..1825
                     /locus_tag="ACHK07_005150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105332.1"
                     /translation="MQPNTGAHSSTFGVPENMQSVLSNADELLLIHLTKEKRSESLVC
                     LNIQQNPQGVSRSVPGVYESHNIDRDDIYEWLFYPMVVLCVPVNGGLPLDR"
     gene            1960..2493
                     /locus_tag="ACHK07_005151"
     CDS             1960..2493
                     /locus_tag="ACHK07_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105333.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2767..3462
                     /locus_tag="ACHK07_005152"
     CDS             2767..3462
                     /locus_tag="ACHK07_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO8105334.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            3557..3688
                     /locus_tag="ACHK07_005153"
     CDS             3557..3688
                     /locus_tag="ACHK07_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105335.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            3700..>3775
                     /locus_tag="ACHK07_005154"
     CDS             3700..>3775
                     /locus_tag="ACHK07_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8105336.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRD"
BASE COUNT         1015 a          798 c         1030 g          932 t
ORIGIN      
        1 ctggcgcagg cagattgata aaagggccgc agagaaaatc agcgccagtc agtcagccgg
       61 aagcaaaccc gaagagccca ttactcctta ataacccctt tcaacgagaa aatcccatgt
      121 cagaaattac atccctggtc actgcagagg cagtgaagga agtcctgcgc tctgaagaag
      181 tcctgagcgc actgaaacag aaactccgcc agaaccttga ggcgcgtctt gatgcagaag
      241 tggatgccat tctggatgaa ctgctgggcg caccggcagt tccggagccg gaaggtatcg
      301 cgggtgaggg gagtgcttca gatggcggtg accccacacc tgacagcgac atgatgatgt
      361 aagcatgcgc aagggaccat cggtgtgtgc cggtggtctt tatattgttg tgagcttccg
      421 gattgcggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt gtcatacacc
      481 acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct
      541 cagtgggcgg caataggtgt gctggggagt ctgctgtttg gtctgctgac atatctgacg
      601 aacctgtatt tcaaaatcag agaggaccgt cgtaaggcgg cgcggggaga gtagacgatg
      661 aataaaaaat atgaactggt tgtaaaaggg ataaatcatt actcggataa gataactgtt
      721 actgtgacac tggaaattgc cgggcagccg tcgttgttgt cgccatatgt ggcgattagt
      781 cttgaccgta ctgaaggtgc cacgctggaa ttttacgaag ctgaggcgaa aaaacaggcg
      841 aagcagtttt tcatggatat tgctgccgct ttatgtgaag gggatgaaca gtcacaggaa
      901 aagcgcctct gttcagaaga gcggtatacc atacaaatta acaatgcata taacacgata
      961 cagtcagaaa aagatgatat cgaatcacgc attgaaaaac tggagaacag cgtcgttgaa
     1021 ctgaataaaa aactgtcggt gcagatccct tccgaaggtg aaaaaaaacg ccgtgatgag
     1081 cagttcgcgg cgttttacga ttattgccgg aaagtgatga gcagaaatct cgcagagtgt
     1141 ttccgtattc aaaaaaacag agagcgctct gcaaatagtg agattctgga taatttttta
     1201 aaggggacgc atctcgccag tgattgtttc attgctcatt cttttggtgg ctttcatgag
     1261 gccatcaaac atgatgttga gagaagtcca ggtatcctct gttccattcc cgtaagcatc
     1321 gaaatagaca caaatacgat aaccggtgaa ctcgttaccg ccggagtcgg tatattccgt
     1381 catgagggga acgatgccaa aggttccttt acaaaaatcg ggccgttctt tctgaaggta
     1441 gagcgatagt ttgttaagta cccatccgac agctctctca ttttggttta ttttcgtatc
     1501 gcgaaacaaa aattcgaagc ggccagatga cgcatgggtg caaccaaata ccggggcgca
     1561 ttcatcaacc tttggtgttc ctgaaaacat gcagtccgtg ctctcgaatg cagatgaatt
     1621 gctgttgatc cacttaacaa aagaaaaaag gtctgaatcg cttgtctgtc tgaatattca
     1681 acaaaatcct caaggtgtgt ctcgatcagt tccgggtgtt tacgaaagtc ataatattga
     1741 ccgggacgat atttatgagt ggttgtttta tcccatggtt gtgctctgcg tccctgtaaa
     1801 cggaggtctg ccgctggata gataagcata agtaatctcc tggtggaatg taatccacga
     1861 tcatccgggg aaattaagaa cccgccagtg cccaccactg gtggggtgaa ggcttaacat
     1921 atccgggtat tcggaaccga taaatcctga taaatatcca tgaacgcaaa aatcagatac
     1981 ggcctgtcgg ctgccgttct ggcgctgatt gccgcagggg cgcctgcgcc tgatattctc
     2041 gaccagtttc tggatgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt
     2101 atctggacca tttgtcgtgg tgccaccagg gtggatggca aacctgtcgt cccgggcatg
     2161 aagttgtcga agaaaaaatg cgaccaggtt aacgccattg agcgtgataa ggcgctggca
     2221 tgggtggaga aaaacatccg ggtgccactg accgaacccc agaaagcggg gatcgcgtca
     2281 ttctgcccgt ataacattgg tcccggtaag tgtttcccgt cgacgtttta taaacgaatt
     2341 aatgcaggcg atcgcagggg agcgtgtgag gcgattcgct ggtggattaa ggacggtggc
     2401 agagactgcc gtattcgctc aaataattgc tacggtcaga tatcccgtcg tgaccaggag
     2461 agtgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt
     2521 tggtcaacgc gggtggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaag
     2581 tcaggaacat tgtttcacgc agaggcaccg taatggtgcc tttgtcattt ctgcgcttcg
     2641 cacaagcgta aataaaccaa agaacctttc aggatgagcc ctggtggata accggcagtg
     2701 gtctggttaa ccctctttgg gctggttatt cctgtgcgca gggttcatca ctaaaaggaa
     2761 tcaaccatga aagagatgat ttctgtcgat cgtgaaatat ccatgagtag tctggatttt
     2821 ctgaataaca ttattaatcc agcccgggca gaagccggag aagtccctca tgaaccgcgt
     2881 aagtttcttg caaaaattga ggatgagcta gagcttgatg gaaccggaaa aaaattccgg
     2941 ttaaacaata accagacaag aacggcatac tatgatctgg attttgacca gatgatgctc
     3001 gttggcatga gagagtcaaa ggccgttcgt cgttctgtgc tggcaaggct caaagcgatg
     3061 catggtattc agatccccca gactttacct gaggcgttgc gatttgcggc aaaactggca
     3121 gaacagaaag cagagctgga aaatcaactg gcaatagcag caccgaaggc tgaatttgtt
     3181 gataactatg ttgaagcatc tggtctgatg ggattccggg aagttgctaa gttactcggt
     3241 atcaaagaaa ccgatttccg gctgtttttg ttggagaacg gaataatgta tcgcctcgct
     3301 ggaaaaatga cgccttactc gcatcacctg gatgcggggc ggtttagcgt gaaaacgggc
     3361 gaggcgggca atggtcatgc ttttacgcag gttaaattca ccccaaaagg tgttcagtgg
     3421 attgctggtc tgcttgctgc atggagagct accgcagcat gaagatgata aaaatggact
     3481 ggaaattttt gctggtctgg ctgattccgt ttttatgggt ggttgcccgg ttaattactg
     3541 ctattaagag gtaaagatgt cagacaaact cataatgctg gcgaagggcc tttgtgtaat
     3601 cgtcggtatt tcattttcac taatgctggt tgctcttttt ctttccataa cctggatggc
     3661 gttgacttcg gcagggctgg tggggtgagc ataaaccgaa tgctttccgc gtttaccgtt
     3721 attctgctgg tggtctgtgg tgcgcttagt ctggggctga atcattaccg cgata
//
LOCUS       DAZGCQ010000239         3359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000239 DAZGCQ010000000
VERSION     DAZGCQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.242"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1726
                     /locus_tag="ACHK07_005155"
     CDS             <1..1726
                     /locus_tag="ACHK07_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105337.1"
                     /translation="VPGAVMRLSCATWWNSQLRHLRDIWREGLLRAAGLVSRKTSVYI
                     SHEALLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMMACVRG
                     MEQVAEARGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYRFFAAVRR
                     AINKAGLGWYGVRTVEPHHDGTPHWHMLVFTSPENEARITEIMCNAAIREDRAELGDD
                     ISPRFKCEKIDPAKGTPASYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETV
                     ENAIGWAALHRIRQFQFFGIPPRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAA
                     DVGCFATYITRQGGVLIPRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPSLGERYTI
                     CTHPDTWKLVRRKPENEDRTDEGFDVECGFAAPWTRGNNCPPDQNLTINDPPERQPDG
                     RREQRLTLPEGDLGEWLRSLTPTERKQLTRQLKNPPPDTETVKNGGISAPAGGVRVSV
                     ALTAEPHITARITRELDALGVQLHESAVVSVRHGARVRLADGRTAYWNDSTRRIDITP
                     AQNTEAPQ"
     gene            1723..2127
                     /locus_tag="ACHK07_005156"
     CDS             1723..2127
                     /locus_tag="ACHK07_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105338.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATRPQPGDELEITIV
                     SPDRTTTAIITDILNGRPDNTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            2176..2346
                     /locus_tag="ACHK07_005157"
     CDS             2176..2346
                     /locus_tag="ACHK07_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105339.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            2343..2690
                     /locus_tag="ACHK07_005158"
     CDS             2343..2690
                     /locus_tag="ACHK07_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8105340.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAGGDLPVISAQ"
     gene            2714..2971
                     /locus_tag="ACHK07_005159"
     CDS             2714..2971
                     /locus_tag="ACHK07_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8105341.1"
                     /translation="MTTQIATRGRLAVSLPCHGAEQPTTMWLYLITFGKQHEELAKRK
                     KGDLVQVHGNLQVNRYQDKFGQQKEGWQLLAQSIISSRTAR"
     gene            3091..>3359
                     /locus_tag="ACHK07_005160"
     CDS             3091..>3359
                     /locus_tag="ACHK07_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105342.1"
                     /translation="MKHNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPNQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGHITVYDHIHRTSS"
BASE COUNT          919 a          969 c          909 g          562 t
ORIGIN      
        1 ggtgccgggt gcggtcatgc gcctttcctg tgcgacatgg tggaacagtc agttaagaca
       61 cctccgggac atctggcgtg aagggctgtt acgcgccgca ggtctggtgt cacgtaagac
      121 atcggtatat atcagccatg aagcgttact ggactggcgc gaaaaacgcc agcgcacccg
      181 cgattttctg aaggcctggg acatcgagaa cgaagaggga gaacggttca gccttgagga
      241 tatctactgg gcaggtctct ctaatccgcg taaccgccgt aacgagatga tggcctgcgt
      301 ccggggaatg gagcaggtcg ccgaagcacg cggcgacgtg gcgtttttcg tcaccgtcac
      361 agccccttcc cgttttcaca gcgtgaacga tgacggcagt ctgaacccga aatactgcgg
      421 ggcaacggta cgtgatgcca gtgattatct ggtttaccgc tttttcgccg ccgtccgcag
      481 ggccatcaac aaggccgggc tgggctggta cggcgtgcgc acagtagagc cccaccacga
      541 cggtacaccg cactggcaca tgctggtatt caccagcccg gaaaatgaag cccgtatcac
      601 tgaaatcatg tgtaacgccg ccatcaggga ggaccgcgca gagctgggtg atgatatctc
      661 cccgcgtttt aaatgcgaaa aaatcgaccc ggcaaaaggc acaccggcaa gctatatcgc
      721 gacctatatc ggtaagaacc tggacgccag cgccttcatg gggaatgacc cgaaaaccgg
      781 caaaccttac gtcgataagg aatcaggcaa aaccatggcc gaaaccgtgg agaacgccat
      841 cggctgggcg gcccttcacc gcatccgcca gttccagttc ttcggtatcc cgccccgcca
      901 ggtatggcgc gaactccgcc gtcttgccgg gcagatggcc cgtaacccgg cagccccgca
      961 gcgtctggac catgacgaca ttgacgccat actggccgcc gccgacgtgg gatgttttgc
     1021 cacctatatc acccgccagg gtggtgtgct cattccccgc aacacgtacc tggtccgcac
     1081 cgcatacgaa actgccgaag aggccaacga ctacggcgaa ttcccgcaac gcatttacgg
     1141 cgtccgcgcc ccgtcactcg gggagcgcta caccatatgc acccacccgg acacctggaa
     1201 actggtcaga cgtaagccgg aaaacgaaga ccgcacggac gagggttttg acgttgagtg
     1261 cggctttgcc gcaccttgga ctcgtggcaa taactgtccc cctgaccaga atctgaccat
     1321 aaacgacccg ccagaaaggc agccggacgg acgcagggag cagcgactta ccctgccgga
     1381 aggtgatttg ggtgaatggt taaggtcact gacccccaca gagcgaaaac aactcaccag
     1441 gcagctgaaa aacccgccac ctgacacgga aaccgtgaaa aacggcggca tcagtgcgcc
     1501 cgctggcggc gttcgcgtga gtgtcgctct gaccgctgaa ccacacatca ccgcacgaat
     1561 cacccgcgag cttgatgcgc tgggcgtgca gctgcatgaa agcgccgtcg tttccgtccg
     1621 gcacggtgcc cgcgtgcgcc tggctgacgg gcgcaccgcg tactggaatg acagcacccg
     1681 ccgcattgat atcacccccg cacagaatac ggaggcgcca caatgacccg cttttattgc
     1741 gccatgcagc gacagcgttt tgacgaaatg atgcgccagc gcacgccctt actgaccctg
     1801 aacaacattg ccacgagacc gcaaccaggc gatgagctgg aaatcacgat cgtcagtccc
     1861 gatcgcacca ccacggccat catcaccgac atactcaacg gacgaccgga caacactaac
     1921 cgcagctggc ttgtcgctgt tcgtccgcgc cgtccgcccg gagagccgga gccgctcacg
     1981 cctgccggaa aaatcatgcg gcagtaccac gaagagcagg cacacaaaca caacgcagaa
     2041 ctgaccagaa cactggcatc gattaaccgc aaagcggcaa ttgcccgcta tgtgcattca
     2101 gccgctggca gacaatcacg caggtaaaca aagaccatga acataaccag aaacagccaa
     2161 ctaaaggagc gcaccatgaa cagcaccagg aggatcagcg acagagagct tacgaaaatg
     2221 attgagtcgg cacagcagct gctttcacga caaaacccgg tcgtggaaga aacatggtgg
     2281 gagaatttac tggcagcact gaccgaactc cgggaacgcc ggaacaacga aggagccgcc
     2341 aaatgaagac cccaggaatg accggaaagg aaatcatttt acgctacctg gaaacgcacg
     2401 cacgtttcag cgccccagaa ctggtagaaa aactgggcgt gaaaagaacc gtcattaatc
     2461 aggccgccgg aaaaatggta cgggaagggt tgctggttgt ggacgggtac acggacaaga
     2521 taacgcatta ccggaagcca acggaaaagg aacgacagga actggcgcaa cgggctgaac
     2581 ggcagcagga aaaatcagtc atcgaagcct gcaagcgcag cgaaatcatg aagcgaattc
     2641 tgtttatcta cggcgctggt ggagatctgc ctgtcatatc agcccaataa ctaaaccacc
     2701 aacggagaca aatatgacaa cgcagattgc aacacgggga agactggcgg tatcactgcc
     2761 ctgccatggc gcagaacagc caaccacaat gtggctttac cttatcacat tcgggaagca
     2821 gcacgaagaa ctggcaaaac gcaaaaaagg cgatctggta caggtgcacg ggaacttgca
     2881 ggtaaacagg tatcaggaca agttcggaca acagaaagag ggctggcaac tgctggcaca
     2941 aagcattatc agctcaagaa ccgcgcgatg acttcacgca tgttaccctc actgttgagg
     3001 gtaactacga cgaccgcaca gacaacccag cttgataaag aaaaaaatga tgttatgatt
     3061 aagtcataag taaggtgata cgaatcagtt atgaaacaca atgatcatgg gctgaacacc
     3121 ctcctggata tgcatggata ccagcaccac atggataatg gttactggtg gaagattgaa
     3181 gcatacaggg ttagcccaaa ccagttcagg ccacatggaa tacgctacaa cctgacattg
     3241 cacgatcctt ataactcccg ggtatttgga atggataacg cccacggaat aaagccacca
     3301 aagaaaggga aatttaccgg gcacattacc gtttatgacc acattcacag aacatcgtc
//
LOCUS       DAZGCQ010000240         3297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000240 DAZGCQ010000000
VERSION     DAZGCQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.243"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3209
                     /locus_tag="ACHK07_005161"
     CDS             <1..3209
                     /locus_tag="ACHK07_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105343.1"
                     /translation="TALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRN
                     PSSVRLLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRMVRETADST
                     TDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVPS
                     NYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIG
                     QYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDR
                     PSDVVWPYTNSDVVVDDNGVGFRYSFSAMKDRHTAVEVNYTDPQNGWQTSTELVEDPE
                     AILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDII
                     EICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITA
                     HPAPDRIQVSVLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEK
                     EAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLAQAKWDTPRVVKGVRFSL
                     RLTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINA
                     PAAPASIELTPGYYQITAVPVLAVYDPTVQYEFWFSEKRITDMAQVETFAHYLGTGSQ
                     WSVSGPHIKPGKDFWFYVRSVNLVGKSVFVEASGRASNDAEGYLGLFREKIGKLHLAQ
                     GMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDL
                     AALYMLKVQKTKNGIPYVAGIGAGIEDADGRPLSNILLQADRIAMINPENGNTTPLFV
                     AQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNN
                     VVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIII
                     PPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGHGHI
                     TLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          769 a          875 c         1028 g          625 t
ORIGIN      
        1 aaaccgcact gggcgtggag gtgacgaagg caaagccggt gacgcgcacc atcacgtcag
       61 cgaacattga ccgtctgcgg gtcaccttcg gggtacagtc actggtggag accacctcaa
      121 agggtgaccg taatccctct tctgtccgcc tgctgattca gcttgagcgt aacgggcact
      181 gggtgacgga gaaggatgtc accattaacg gcaagaccac ctcgcagtac ctgacgtcgg
      241 tgattctgaa taatctccct gagcgcccct ttaacatccg gatggtcagg gagacggcgg
      301 acagcaccac ggaccagctg cagaacagaa cgctgtggtc gtcatacacc gaaatcatcg
      361 atgtgaaaca gtgctacccg aacacggcga ttgtggggct gcaggtggat gcggagcagt
      421 tcggtggcca gcagctgacg gtgaactacc atatccgtgg tcgcatcatt caggtgccgt
      481 caaactatga cccggaaaaa cgcacctaca gcggtatctg ggacgggagt ctgaaaccgg
      541 catacagcaa taacccggcc tggtgcctgt gggacatgct gacccacccg cgctacggca
      601 tgggaaaacg cctgggggcc gcggatgtgg acaagtgggc actgtatgcc atcgggcagt
      661 actgtgacca gacggtcccg gatggtttcg ggggcacaga gccgcggatg actttcaatg
      721 cgtacctgtc acagcagcgt aaggtctggg atgttctggg ggatttctgc tcggcgatgc
      781 gctgtatgcc ggtatggaac ggccagacgc tgacgttcgt tcaggaccgt ccgtcggatg
      841 tggtgtggcc gtacaccaac agcgatgtgg tggtggatga taacggcgtg gggttccgct
      901 acagcttcag tgccatgaag gaccggcaca cggcggtgga ggtgaattac accgacccgc
      961 agaacggctg gcagacctcc acggaactgg tggaagaccc tgaagccata ctgcgctacg
     1021 gacgcaacct gctgaagatg gatgcgttcg gctgtaccag ccgcggtcag gcccaccgtg
     1081 ccggactgtg ggtgataaag accgaactgc tggaaacgca gacggtggat ttcacgctcg
     1141 ggtctcaggg gctgcggcac acacccggtg acatcattga aatctgtgat aacgactatg
     1201 ccgggaccct gaccggcgga cgcatcctgt ccatcgatgc cgccagccgc acactgacgc
     1261 tggaccgtga ggtgaccctg ccggagacag gtacatcgac ggtgaacctg attaacggca
     1321 gcggtaagcc ggtgcgcgtg gacatcactg cacaccccgc cccggaccgg atacaggtca
     1381 gcgtcctgcc tgatggcgtg gcgacatacg gtgtgtgggg actctccctg ccgtcactgc
     1441 gtcgtcgcct gttccgctgt gtttccatcc gggaaaacac ggacggcacc tttgccatca
     1501 cggcggtgca gcatgtgccg gaaaaagaag ctattgtgga taacggggcc cgctttgagc
     1561 cgatgtccgg ctcactgaac agcgtcatcc cgccggcagt gcagcacctc acggtggagg
     1621 tgagtgcctc agacggccag tatctggcac aggcgaaatg ggacacgccg cgggtggtga
     1681 agggcgtgcg cttcagtctg cgcctgacca gtggcagtgg tgaaaacagc cgcctggtga
     1741 ccagcgccat cactgccgac acggagcacc gtttcagtgg cctgccgccc ggggaataca
     1801 ccctgacggt cagggcgata aacagctacg gccagcaggg cgaacctgcc accactacat
     1861 tccggattaa tgcgcctgca gcaccggcca gcattgagct gacgccgggg tattatcaga
     1921 taacggcagt accggtgctg gcggtgtatg acccgacggt acagtatgaa ttctggttct
     1981 cagaaaaacg catcacggac atggcacagg tggaaacctt tgcccattat ctggggacag
     2041 gcagccagtg gagtgtctcc ggcccgcaca ttaagccggg gaaggatttc tggttttatg
     2101 tgcgcagcgt caacctggtg gggaaatctg tgtttgtgga agccagtggc cgggccagca
     2161 atgatgcaga agggtatctg gggctgtttc gggaaaagat aggaaaactg catctggcac
     2221 aggggatgtg ggagctgatt gataacagcc agcttgatga tgagatggca gagatgaaga
     2281 ccaccatcac agaaacccgc aatgaaatca cacagacggt cagtaaaacc ctggaagacc
     2341 agagtgccac catacagcag atacagcggg tgcagacaga cacaaataac gacctggctg
     2401 cgctgtacat gctgaaggtg cagaaaacga aaaacggcat tccgtatgtt gccggtatag
     2461 gtgcggggat tgaggatgct gatggccggc ccctgagcaa tatactgctg caggcggacc
     2521 gtatcgcgat gattaacccg gagaacggca acaccacgcc gctgtttgtg gcgcagggga
     2581 atcagctgtt catgaacgat gtgttcctga agcgactgtt tgcagtgagc atcacctcgt
     2641 ccggcaatcc cccgacgttc tccctgacgc cggagggcag gctgacggcc cgcaatgctg
     2701 acatcagcgg acatatcagt gcgaactcgg gcacgctcaa taatgtcgtg atagcggaga
     2761 actgtacgat aaatggcacg ctgaaagcgg agaacattat tggtgatctt gtgaaatgtg
     2821 caggggtggc ttttccggtg gatggtagtt accttgcgaa cggtacacga acgctgacgg
     2881 tgtatgacga tcacagcttt gaccggcaga ttataatccc gccgataatc tatgtcgggt
     2941 caaaacagga atcccgtacc agtaatgaca tctggacaga gtgcttcctg catgttgatc
     3001 agaacggacg ccggatttat tccggcaggt cagtgacaga gccgggaatt ttcagcggga
     3061 tcatcgatat gccagccggt catggtcata tcaccctgag ttttaccgta agttcacgac
     3121 gtcagaacgg gagttttggc agttcacgta tcagtaacct tcaggcgata gtggtgaaga
     3181 aaaacagcgc ggggatcagt atccgctgaa catcgcgccc cgggattgcc ggaaggagac
     3241 aaaaaccgta cagtatgcgc gggtgccttt ggctgatggc cggagggaac acctgaa
//
LOCUS       DAZGCQ010000241         3295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000241 DAZGCQ010000000
VERSION     DAZGCQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.244"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..293
                     /locus_tag="ACHK07_005162"
     CDS             <1..293
                     /locus_tag="ACHK07_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO8105344.1"
                     /translation="SLVMGCTQHKTDMPRQLVKALPQYPAYAAANYIKGRVDVKFDIG
                     ADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFIFSPSAP"
     gene            complement(425..625)
                     /locus_tag="ACHK07_005163"
     CDS             complement(425..625)
                     /locus_tag="ACHK07_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8105345.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            667..1032
                     /locus_tag="ACHK07_005164"
     CDS             667..1032
                     /locus_tag="ACHK07_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8105346.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1315..1872
                     /locus_tag="ACHK07_005165"
     CDS             1315..1872
                     /locus_tag="ACHK07_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8105347.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1869..>3295
                     /locus_tag="ACHK07_005166"
     CDS             1869..>3295
                     /locus_tag="ACHK07_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8105348.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQSWKLGGA"
BASE COUNT          745 a          773 c          998 g          779 t
ORIGIN      
        1 cttctctggt tatgggctgt acgcaacata aaacagatat gccccgacag ttggttaagg
       61 cattaccaca atatccggcc tatgcagcgg caaattatat aaagggacgg gttgatgtga
      121 agtttgatat tggtgctgat ggtactgtca cccgaattga gtttatccgt tcagagccgc
      181 accatctgtt tgatgagcag gttgtaaagg cgatggcaaa atggcgattt gagaaggaca
      241 ggccgcgtaa aggcgtgaag aaaacgttta tctttagtcc ttctgcaccc tgattatttc
      301 atcagaaatt aattatcact ctgttgttat tctgtacatc ccggctgggt aagtcttgtt
      361 ccgccgggta tgaagatgaa atattgttgg aggacagtgg gtacctgctc ctgtaaccga
      421 acgttcattt ctcgttattt gtcatgctgg ccgggcgcag gtgcgttgca tctgttgcca
      481 gccttctccg gcaggcttca ataacccacg ctgaaaagtt accggaccct ttatgctcaa
      541 gggcgatgtt gatctgttca atcatgtgat tggggaaacg gatattgcgg gttgtggttc
      601 tgcgggtccg gtttttcgat gacatattta tttcctttac tgattgcctt atgacgggga
      661 ttttacatgg ctgagcttcg tacactccag agcagaatca aaacactgaa tacccgacgg
      721 gtgaatattc tgaagggtga acagcgtcgt gtcagtggca gtgcacgtgt ttccctcaag
      781 cgtcatatct ggctcaggga tgccgggcag tgctgtctct gtggtcgtgt ggttgacctc
      841 tgtgacagtg aactcgatca ccgaattgca cttcagttcg gtggtggtaa tgaggagacg
      901 aatctctgga cgctctgtac cgaatgccat cgacaaaagt ctgctcgtga agcggcgggt
      961 ggtatgccgg acccgacact gccggaggtg tccggaggta gtggcagagc ggacgacatc
     1021 atcggactgt aacccgaccc ggggggtatc atccggcgta aaaaacgatc gctccggaca
     1081 ccgcgccccc tctcacgcag agaaaaaatt ccccttcaga agttataaac atgtttataa
     1141 cctactgatg gatttttacc gtttttttct ttttattcat aacgttgtgg gaggtaaatg
     1201 tttatacctt tttttcgcaa aatgtttaca gggcagcggt aaacattttc cctgccgcct
     1261 ccattcttac cgggaggatt gctgacgttt ttctgttttt ccccggagga aagaatgctg
     1321 acgacgcaaa aaagaaaatt tgcgctggcg ctcatgtcgg gcaaaaacag aacggctgct
     1381 gctattgagg ctggttactc ggaaaagtcc gccagatgta aaggctcaca actggcaaaa
     1441 gatccggaag tgctcgcgtt tatagcccgt aagcagggcg agacggtgga ggtggatgag
     1501 gttcctgttt accggcagaa aaagacgcag gcgtgccacc gggaagttcc tccggtggaa
     1561 aagaaaaacc aggtttcagt gccaccggat gacgggcaaa gtttctctcc actatccggt
     1621 attgattaca tggaggacgg ccttcctgat cctgtgaaag ccatgggtcg gatcctggtg
     1681 gaaaacatca gcattgatcc gaaactggct ctggatgcag cctggcgact ggcgcagttc
     1741 acgcaccaca aaaaagggga tgccggtaaa aaatcggcaa aaggtgacgc ggcgaaaaaa
     1801 gcggctaacc gttttgcggt gccaccacca ccccgcctgg tggtgaataa tgataatgag
     1861 ggcaacggat gatacctgtg tggagcacgg cctgcccgga ctgggcagag cgtctgaaaa
     1921 aggggctgtc gattattccg gctccgattt acccggacca ggccgcacat gccctggcga
     1981 tttttaaaca actgcggatt gtggatgcac cgggcagccc gacgttcggg gagtcctgtg
     2041 caccgtgggt gtttgatctg gtggctgccc tgtttggctc ctacgatgcg cagaccggtg
     2101 tacgccatat aaaggaagtg ttcattctga tacccaaaaa aaacagcaag tccacgctgg
     2161 ctgccgggat catgatgacg gcgctgttac tgaactggcg gcaggcggcg ggctacacca
     2221 ttctggcccc gaccgtggag gtggcggcta acgccttcaa ccctgccagg gatatggtac
     2281 gacgggacga tgatctggat gacctctgtc aggtgcagac acatatccgg accatcaccc
     2341 atcgggtgac ggacaccacc cttaaggtgg tggctgccga tccgaatacg gtgtccggta
     2401 tcaagtccgt ggggacgctg attgatgaac tgtggttatt tggcaagcag tacaaagcgg
     2461 aggacatgtt acgtgaagcc atcggcggcc ttgcctcccg cccggaaggg tttgtggtgt
     2521 acacaaccac ccagtcgaat gagccgcccg ccggggtgtt cagacagaaa ctgcagtacg
     2581 cccgggatgt gcgcgacggc aaaattaatg atccgcactt tctgccggtg atatttgagc
     2641 atccgccgga gatggtggag cgcggtgagc acctgctgat ggaaaacctc gccatggtta
     2701 acccgaatct cggttattcg gtggatgagg cttttctgta ccgggagtac cgtaaagccc
     2761 gggaggctgg tgaggaggca tttcgtggct tcatgtcaaa acacgccaat gtggaaattg
     2821 gtcttgccct gcgttctgac cgctgggcgg gtgcggattt ctgggagcag cagggcaggc
     2881 gcgtcagcct ggacgatatc ctgcagcgcg ctgatgtggt gacggtgggg attgacggcg
     2941 ggggcctgga tgatctgctg ggaatgtacg tgattggtcg tgacagggaa acccgcgaat
     3001 ggctgggctg gggccatgcc tgggcgcatg aaaccgcggt ggtcagacgg aagagtgagg
     3061 catcccggtt tcaggatttt gtggccagtg gagacatgac gattgtccgt cgggtcgggg
     3121 atgacacggc ggaagtggcg gagtatgtgc gtcgtattca tgaggctgag ttactggacc
     3181 atatcggtat tgacccgtca ggtgtggggc agattctgga ttcactggca gaagccggga
     3241 tccccgacgg aattgttgtg gggataagcc agagctggaa gctgggcggg gccat
//
LOCUS       DAZGCQ010000242         3283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000242 DAZGCQ010000000
VERSION     DAZGCQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.245"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(226..603)
                     /locus_tag="ACHK07_005167"
     CDS             complement(226..603)
                     /locus_tag="ACHK07_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105349.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DVSKFLGDKSKSHALVWKNFGQE"
     gene            complement(603..2369)
                     /locus_tag="ACHK07_005168"
                     /pseudo
     CDS             complement(603..2369)
                     /locus_tag="ACHK07_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(2575..>3283)
                     /locus_tag="ACHK07_005169"
     CDS             complement(2575..>3283)
                     /locus_tag="ACHK07_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO8105350.1"
                     /translation="DFFSKPKNYHFKSKLNYYPITQAAFLGETEVVSNLIKLGVDLDA
                     RGDLGRTALHEAVSNGYFDIVKLLVESGADLTIKDEFGKTALELAEVHQNYKIVEWLS
                     HYRDHNPIPDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIAVRKRRQLDVRCFIQ
                     HGADINAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPMDLAILMG
                     DKRIIFYLYGYISHVSE"
BASE COUNT          818 a          951 c          604 g          910 t
ORIGIN      
        1 atttaaatat atgatacaca tcattaaata tataaccata ctttttcata aggcaatcta
       61 acttagctct taaatcacca aaacccatca caccataaca tctaatagcc attgccacac
      121 gtcaccatgg atttatttcc acctgcaatt acttcgtcct aactttaatt taatctcaag
      181 aagcattaag atctcaatag cgagcttaat acttcttgaa taatatcact cctgaccaaa
      241 atttttccac actaatgcat gagactttga tttgtcaccc agaaatttac ttacatcttg
      301 ccaaagtaaa gaattgctat cggtcgcagt gactattgcc tctataagcc ctgtagctac
      361 aactgtggcc aattcatcat caagcgagac cataccatct tcgacatgtt taaatatttt
      421 ttctctttca atatccccca tttcataaaa attttttaca atcgtttttc caacctgaga
      481 gaacaacata accaccggtg gcgtttctgg atgccagtat tcattagcta atgagactgt
      541 ctcagacaaa acagtggaaa atgatgccat tttttctata taactagcaa tatattggtt
      601 cattacttgc ctcccaaagc attctgtaaa tcctgaagaa cgttttctgc tgctgctcga
      661 tcccctggag atgctgttgg attcttatcc aaccaatcct gtaaagccct tgtataatcc
      721 tgtgctttca atacatgtcc tctaccacca actttttcac ctgtggccaa ttcataacga
      781 acagcatctg ctgtgcttcc acttcctact tttgcatttg gccgataaag atcgtctata
      841 atatttttca aatttgaatc tgtaacgtcg ggttttccca ttttctgtcg caattcatct
      901 ttataccgtt catgctgcat acgatccctt gcgctattct catcttctgg tccccatcct
      961 cccgggcttc ccgtactgcc gccggccagg cagttcttct cttcatccgt caaatccttc
     1021 ccaacattcg gctgactccc tgacgaatta tcattctcat gtgggctatc taaaggacaa
     1081 ctactggtcc agtaacacat ttcagcctga gaatttatta actgcccatc gctcagagcc
     1141 ttcccggcac gcccatcatt agcgagaagg tcatatccca ttggcccagc gaccacagcc
     1201 attgctccaa gagcaggggc agcagacgcc atagccgccg tgactgcatc aaaagtcgcc
     1261 gccccacccg aaagtaacgc ccccccaaat tctgttcctg ttactgttgt ggcagaacca
     1321 agaaccagtg tctcaccaga caacccaaga ggatcccccc gctgcaccgg gttcagcgga
     1381 tacgcataca ggttccatcc ccccttcagc ccaatgggat cctgggtgat atagcgtccc
     1441 tgcaacggat cgtagtaccg gttccggtta tagtacagcc ccgactcctc atcatactgc
     1501 tgccccggca gacggagtga ctgttgaagg tgctgcgcgc tggtttcgcc cagcaggttt
     1561 ccccactcgt catactcccc ctgccacgcc gtttcccctt ccgggctgat gagcgccagc
     1621 ggcagtcccc ggtggtcgca gtggtaaaga tgaagtttcc tctccgggat gtattccgcc
     1681 tccagctgtg cggccatctg ctccgccgtc agcccgcact gcgcaagcca tgcctcactc
     1741 tccgcactca ccgctcccgc gcgcagttcc cgctccagcc ttcccagcat caccgccagc
     1801 tccgccggta gcgtcacccc cgtgtcctcc tgcaacacct ccgccaggct acggtgccgc
     1861 gccttcgcct gttcgcaatt ctctgtttcg attctgatga gtggtgtgaa gctccccggc
     1921 tggtataccg tctggatacg cgtggtgtcg gtctgtaccg tcgtcagcct gtcgccgtcc
     1981 cacccgtacc acgtctcctc cggtttacgc gacagcgaca tccaccccgt caggtcacgc
     2041 tcccgccgcc acacccgttt tcccgtccgg cggcccagcg ggtcatagag gtaccggctc
     2101 tccacctgtg gttcgccgtg ctgtatccgc gtgtagaaca ccaggcggtg ctggctgtcg
     2161 tagtgatagt ggtgggtgcg ctcgtcgtgc atccggataa ccccttccgg gatacggtcc
     2221 gtcttctccg ccagcctgcc gtattcatcg tagcggtaga catagtgcgc atcttccgcg
     2281 atgcggttat ccggccacgc cgtgagcgtg ctgtccggat gcagttccgg gtccggcagc
     2341 cggtttcccg ccgggtctgt tgcatacggg aaattccctt gcagggcgtc cacaacatgt
     2401 gcatatttcc tgcacatgct gtggttttgt catacaagga gcaacaatac tagttaatga
     2461 ttcataaaaa attttaacgg ggtgattttt catgcgatta tcgtcctaat taacgccacc
     2521 tcacttacag attatttcat tcgttacaat cttttgattg cattacaccc aggcttattc
     2581 agaaacatga gaaatataac catataaata aaaaataatt ctcttatccc ccatcaaaat
     2641 agccaaatcc ataggggagt atcccatctc actctccaaa taaatatcag cacctaattt
     2701 caacaaaagc ttcatcatat caaaatcccc tatccctatc gaataatgca aaggagtgaa
     2761 atcaaagcca ttatttgcct tagcatttat atcggcacca tgttgaataa aacatctgac
     2821 atctaattgt cttcttttcc taacagctat atgaagaacc ccctcgccaa gttctgtcct
     2881 tgcatctgta tctataatct ctccaccaaa atatcgctca ccgtagatat taataagtac
     2941 agaaaaatcg ggtatgggat tatgatctct ataatgtgat agccattcaa ctattttata
     3001 attctggtgt acctcagcca attctaatgc cgttttccca aattcatcct ttatagtaag
     3061 atcggctcct gattctacta aaagcttaac aatatcaaaa tacccattac ttacggcctc
     3121 atgaagtgcg gtacgcccca aatccccacg agcatccaga tccacaccca atttaattaa
     3181 attacttaca acctcagtct cacctaaaaa tgcagcctga gttataggat aataatttaa
     3241 ttttgactta aagtggtaat ttttcggctt agaaaaaaaa tca
//
LOCUS       DAZGCQ010000243         3209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000243 DAZGCQ010000000
VERSION     DAZGCQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.246"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..590
                     /locus_tag="ACHK07_005170"
     CDS             <1..590
                     /locus_tag="ACHK07_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO8105351.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNGKGLPNVCRVHGNM"
     gene            562..944
                     /gene="yhhH"
                     /locus_tag="ACHK07_005171"
                     /pseudo
     CDS             562..944
                     /gene="yhhH"
                     /locus_tag="ACHK07_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
     gene            1049..1890
                     /gene="yibA"
                     /locus_tag="ACHK07_005172"
                     /pseudo
     CDS             1049..1890
                     /gene="yibA"
                     /locus_tag="ACHK07_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            1918..2839
                     /locus_tag="ACHK07_005173"
                     /pseudo
     CDS             1918..2839
                     /locus_tag="ACHK07_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2839..3165
                     /gene="ybfB"
                     /locus_tag="ACHK07_005174"
     CDS             2839..3165
                     /gene="ybfB"
                     /locus_tag="ACHK07_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO8105352.1"
                     /translation="MKYIIFSFRAIWLALSLLMLFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVADIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
BASE COUNT         1045 a          518 c          749 g          897 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat ggatatcaat
       61 tgaatccgat atcagacatc gaccccctgg gtttatctat gtgggaggat gcaaaatcgg
      121 gggcatgtac taatggtctt tgcggcacac tatccgctat gataggtcca gataaatttg
      181 attctataga tagcaccgca tatgacgcct taaataaaat aaatagccaa tctatttgcg
      241 aagataaaga gttcgctggt ttaatatgta aggataatag tggcagatat ttctcaacag
      301 cacctaaccg aggagaaaga aaaggatcat atccattcaa tagcccttgc cctaatggta
      361 ctgagaaagt atcagcttat catactcatg gtgcagatag tcatggagaa tattgggacg
      421 aaatattttc aggtaaagat gagaaaatag ttaaaagtaa agataacaat atcaagtcat
      481 tttatttagg tacgcccagt ggtaatttta aagcaataga taaccacggg aaggaaataa
      541 caaacggaaa aggattacct aatgtctgca gagttcatgg taatatgtaa aaaatattgt
      601 ttaggaactg tgtcattgta tctttgtttg tttttacata caacacatgg gcgcagtgta
      661 ataataatat taaaataatg cgcaagtatg aaagtgaagg taaatatacc gttagaaatt
      721 tggttaaaaa taaagctata gcattggaat tagctgagat atatgttaag aatcgttatg
      781 gacaggatgc cgcagaagaa gaaaaaccat acgaaatcac tgagttaaca acaagttggg
      841 ttgttgaagg taccattcac tcagaccaaa ttgctggtgg ggtttttatt atagaaatag
      901 gcaaaaatga tgggagaatt ctgaattttg gccacggaaa ataagtatgt aaggataatc
      961 aaacatcgtg tgcgctgatg gcagagtagg tggaggactc cagacagtca aacgatagaa
     1021 aaagatagcc tttatggagg ttcctgcaat gtcaaataca taccagaaaa gaaaggcaag
     1081 taaagagtat ggtttatata ataatgtaag aaactaaatg atgatgaatt atttcgctta
     1141 cttgatgatc gcaattcctt gaaaaggatt tcatctgcca gagtattaca gttaagaggg
     1201 gggcaagacg ctgttagatt ggcaattgaa ttctgcactg acaaaaatta tatccgtaga
     1261 gatatcggag catttatact cgggcaaata gaaatttgca aaaaatgcga agataatgtt
     1321 tttaatattt tgaacaatat ggcattgaat gataagagcg cttgcgttcg agctacggca
     1381 atcgagtcaa cggctcagcg atgcaagaaa aacccaattt attcacctaa aatagtagaa
     1441 caatctcaaa ttactgcttt tgataaatcg gctaatgtca gacgtgctac ggcatttgct
     1501 atttctgtta tcaatgataa agaaacaatt ccactattga ttaatctgtt aaaagatcca
     1561 aatggagatg tcagaaactg ggccgcattt gcaataaata tcaataaata tgataatagt
     1621 gatattaggg attattttgt ggagatgctt caggataaaa atgaagaagt ccgtattgac
     1681 gcaataatcg gactttccta cagaaaagat aaaagggttt tatctgtttt atgcgatgag
     1741 ttaaaaaaaa atactgttta tgatgatatc attgaagctg cgggtgaatt aggtgataaa
     1801 acgctacttc ctgttttaga tactatgttg tacaagtttg atgacaatga aattataact
     1861 tccgctattg ataagctgaa gcgttcatag cgtgagttgc ctatgcacag tgggggattc
     1921 ccgccggcac ggtgcagatg ctcgaccggc tggaaagtga aatcctggct gagcgggtga
     1981 gtgaggaaag ccgccgctgg ctggcatcgt gcggcctgac ggtggagcag atgcaaaacc
     2041 agatggaccc ggtgtacacg ccggcgcgaa aaatccacct gtaccactgc gaccatcgcg
     2101 gcctgccgct ggcgcttatc agtgcggaag gggctacaga atggtgcgca gaatacgatg
     2161 aatggggcaa cctgctgaat gaagagaacc cgcaccagct gcagcagctt atccgcctgc
     2221 cggggcagca gtatgatgag gagtccggcc tgtattacaa ccgccaccgc tattatgacc
     2281 cgctgcaggg gcggtatatc actcaggatc cgattgggct gaagggggga tggaattttt
     2341 atcagtatcc gttgaatccg atctcaaata tagatccatt aggattagaa acactaaaat
     2401 gcattaaacc actgcattca atgggcggaa ctggtgaaag aagcggtcca gatatatggg
     2461 ggaatccgtt ctatcatcaa tatctttgtg tcccagatgg taaaggggac tatacttgtg
     2521 gtggccaaga ccaacgggga gaatcaaaag gagatggtct atgggggcca ggtaaagcaa
     2581 gtaatgatac aaaagaagct gctggccgtt gtgacctcgt tgaaaccgat aatagttgtg
     2641 tggagaactg tttaaaaggg aagtttaaag aggtaaggcg cgttattctg tattgcctga
     2701 tatattcaca cctataaatt tagggctatt taaaaactgc caagactggt ctaatgattc
     2761 tttagaaaca tgtaagatga agtgctccgg aaataacatt ggacgcttta ttagatttgt
     2821 attcaccgga gtgatgtaat gaaatatatt attttttcat tcagagctat atggctggcg
     2881 ttatcacttt taatgctatt cttttccatg catagacttt cactattaga ctccactcgt
     2941 gatgtgagcg agttaattag tctcatgtct tatggaatga tggtaatatg ttttccaaca
     3001 ggcatagtat tctttattgc tctaatattt atagggactg ttgcagacat tattggcgta
     3061 aggattgata gtaagtatat tatggcgata ataatatggc tttattttct gtcaggaggg
     3121 tatattcaat ggtttgtatt aagtaagcgc attataaaca aataaattaa gttaattgct
     3181 cttattatta tatgtaacct ggacattga
//
LOCUS       DAZGCQ010000244         3185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000244 DAZGCQ010000000
VERSION     DAZGCQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.247"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1590
                     /locus_tag="ACHK07_005175"
     CDS             <1..1590
                     /locus_tag="ACHK07_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO8105353.1"
                     /translation="RRFVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRG
                     VCMDMQLARAAIEAVDQEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDM
                     QRSTLERRIADPHLPSAVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGA
                     SRTGRWAGRLFQPQNLPRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCII
                     APTGKKLVVSDLSNIEGRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPD
                     DVDKHMRQIGKVMELGLGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKS
                     WYNESVKRKSTFGLSERVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTL
                     YCGYLKIRRDGAWLRIQLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKL
                     VENVTQAAARDVLAGNMPLIEDAGYSIVLTVHDEVITEAPDSPDYSAEHLSQLLATVP
                     DWATNIPLNAGGFESYRYRKD"
     gene            1830..2597
                     /locus_tag="ACHK07_005176"
     CDS             1830..2597
                     /locus_tag="ACHK07_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO8105354.1"
                     /translation="MTSQIIPVFNGTIANETTLLCNARDLHAFLGVGKMFAHWIKDRL
                     GEYGFVENQDFVIACQNWQAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECERKLHQQSSTILAPHRECLPKMVYHHSSKYNPYRAYAWDGEKIVYVGCYPTVDEAV
                     AAQERFYQNGSTKRIQKVQTAINDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEK
                     MDSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            2650..2865
                     /locus_tag="ACHK07_005177"
     CDS             2650..2865
                     /locus_tag="ACHK07_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO8105355.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            2862..3161
                     /locus_tag="ACHK07_005178"
     CDS             2862..3161
                     /locus_tag="ACHK07_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO8105356.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPDGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
BASE COUNT          848 a          823 c          840 g          674 t
ORIGIN      
        1 cggcgctttg ttgcttacgc cggactggat atcgaggcaa tgcgcgaagt ctataaacgt
       61 ctgccgaagt ggaattatca ggggacagag ctggcgctct ggcatcgtga tcagcagatc
      121 aacgaccggg gcgtctgcat ggacatgcaa ctcgcgcgcg ctgcgatcga agcggtagac
      181 caggagcaaa agcgcctggc aaagcgtaca caggaaatga ctgatggcga agtgcaggca
      241 gccacacaac gagacgcgtt gattaagcac attgttgaat cctacggtgt ggagctacca
      301 gacatgcaac gcagtactct ggaacgtcgt attgccgacc cccatttacc atctgccgtg
      361 aaagaactgc tggctatccg cctgcaagcc agtactacca gcaccagtaa atacaaggca
      421 ctgatgaaag gcgtaagcca cgacgggcgc ttacgcggta cgctacagtt ctgcggggcg
      481 tcacgtaccg gtcgttgggc cggacggcta ttccagcccc agaaccttcc ccgcccttca
      541 ctaaaacagg aacaaataga cgaaggcatc gaagcactga aagccggatg tgcagacctg
      601 ctgtttgaca atatcatgga actaaccagt tcagcgttac gtggctgcat tatcgcgcca
      661 acaggcaaaa aactggtggt aagtgacttg tcgaacattg aaggccgcat gctggcatgg
      721 ctggcgggag aagaatggaa actgaatgca ttcagagagt atgacgccgg aacgggtccg
      781 gacttatata aactggcgta tgcaaaagct ttcgatattg caccagatga tgttgataaa
      841 cacatgcgtc agatcggtaa agtcatggaa ctcggtctgg gttatggagg tggtgtatcg
      901 gctttcatca cttttgctct ggtttacggt ctcgatctcg acgagctggc gaacgccgca
      961 ctgccaaaca ttccccgcga tgttatccgc gaggcgaaaa gctggtacaa cgaatcggtt
     1021 aaacgtaagt cgacctttgg cctgtccgag cgtgtattca tcgcatgtga ctcgctcaag
     1081 cgcctgtggc gcagggcgca cccggcgacc tgcgatttct ggtacgaact ggagcgaacc
     1141 gtccgcactg caatcgccac accgcaaaaa acattgtatt gcggttatct taaaatccgc
     1201 cgcgatggcg cgtggctgcg catacagcta ccatccggac gcgctgtatg ctacccgtct
     1261 ccggttatcg aacaagggaa tatcacctac atgggtgtta actcttattc gcgtaaatgg
     1321 caacgactca aaacctacgg cggaaagctg gtggagaacg tcacccaggc ggccgcccgc
     1381 gacgttctgg ccggaaacat gccgcttatc gaagatgccg gttacagcat tgtgctgacg
     1441 gtacacgacg aggtgatcac cgaagcacct gactcacccg attactcagc agaacatctc
     1501 agccagttac tggcaaccgt tcctgactgg gccacgaaca ttccactaaa cgccggagga
     1561 ttcgaatctt acagataccg gaaagattga catccgcgat gaccaatagg tatattccgc
     1621 ttcatggtgc tcaaaacacc ttgcaaagcg gaaaccgcac ccgacagtca tgcggatttt
     1681 ttatgtccac gatattgaga attcaatggt cgggcgtgcg gctaatacaa tacccgtaag
     1741 ggaaatacgc ccgccgactt tgcacggttt tgagcgcctg accaccctct caaaaggggt
     1801 gaattcaaaa tattgcaaag gacataaaaa tgactagtca aatcatcccc gtattcaacg
     1861 gcactattgc caacgaaacc accctactct gtaatgcccg tgatctgcac gcttttcttg
     1921 gtgtgggtaa aatgtttgct cactggatca aggacaggct gggtgaatat ggattcgttg
     1981 aaaatcaaga ttttgtgatt gcttgccaaa attggcaagc aaaaggtaga ggcggtcaca
     2041 accgcaagga ctaccacctc accctcgaca ccgctaaaga actggcaatg gtcgaacgta
     2101 acgaaaaagg ccgccagatc cgccgatact tcatcgagtg cgaacgcaaa ctgcaccagc
     2161 agtcctccac aatattagcg ccgcacaggg agtgtctgcc gaagatggtt taccaccaca
     2221 gtagcaaata caacccgtac agggcttatg catgggacgg agagaaaatc gtttacgtcg
     2281 ggtgctatcc gactgtggat gaggctgtcg ctgcgcagga gcgcttctac cagaacggca
     2341 gcacgaaacg cattcagaaa gtgcagacag caatcaatga cgcagagaag gaaatgttca
     2401 tcaacaatct ccgcgccgtc tgtcataact tccggcgcat caatgaaatc tggcgggcgc
     2461 agttaatgcc tgctctcgaa aaaatggatt cgaaactggt ttaccagttg catgaccgct
     2521 tcagcgacag catgtgcatt ctgccgacca tagaagaccg catcggcaga tatatcccac
     2581 ccactttacc tcgttaatcc ttcagttcgc ccctgctcag ccaggggctt tttacaggag
     2641 tcgaaagata tgtcatttaa ataccgggac agtccgcttt attaccgggc tgcgagggag
     2701 gccttgcgcc ttgagcagtc cggcgaatat gaccgggcag cgaaggtctg ggccaaggct
     2761 aaccgccagt cacgtaacga actgaatcag gactggagcg aacgccggtc tgatttttgt
     2821 ctgatgcaga acatgcgcga aaagcgtaag gcggtcgaag aatgatcgtt tacgttgccg
     2881 ggccgatgag cggttacgag caatttaacc gcccggcttt tcattccgca gcaaagaggc
     2941 taacggacaa gggatatgtt gtgcttaacc ccgcaacttt acccgatggt ttgacacagg
     3001 ctcagtatat ggacatctgc ctcgcaatgc ttcgctgtgc cgatacgatt tatatgctca
     3061 aagagtggga gtactctgcc ggcgcacgtg cagaaaacgc attggcagag aaactggaat
     3121 tgaatgtgct ttttgaagag tgggattcag atggcctatg aacgtgaaaa cctaatagag
     3181 aagca
//
LOCUS       DAZGCQ010000245         3065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000245 DAZGCQ010000000
VERSION     DAZGCQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.248"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..713
                     /locus_tag="ACHK07_005179"
     CDS             <1..713
                     /locus_tag="ACHK07_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8105357.1"
                     /translation="TAWYAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEESGLYYNRH
                     RYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNK
                     PPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTC
                     TPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDD
                     LGEILAKMKQRTSRGIRK"
     gene            710..1279
                     /gene="ybfC"
                     /locus_tag="ACHK07_005180"
     CDS             710..1279
                     /gene="ybfC"
                     /locus_tag="ACHK07_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO8105358.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWKWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
     gene            1523..1750
                     /locus_tag="ACHK07_005181"
     CDS             1523..1750
                     /locus_tag="ACHK07_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO8105359.1"
                     /translation="MEILVNSREMALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWK
                     IEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
     gene            2389..>3065
                     /locus_tag="ACHK07_005182"
     CDS             2389..>3065
                     /locus_tag="ACHK07_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO8105360.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHERFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAM"
BASE COUNT         1030 a          558 c          651 g          826 t
ORIGIN      
        1 caacagcgtg gtacgcagaa tacgatgaat ggggtaacca gctgaatgaa gagaacccgc
       61 accagctgca gcagctcatc cgcctgccgg gtcagcagta tgatgaggag tccggcctgt
      121 attacaaccg ccaccgctat tatgacccgc tgcaggggag gtatatcact caggatccga
      181 ttggactgaa ggggggatgg aatttttatc agtatccgtt gaatccggtt cagtatatag
      241 attcaatggg actggcatca aaatatggac acttaaataa tggcggatat ggagcgagac
      301 ccaacaaacc gcctacgccc gatccaagta aattgccaga catagcgaaa caattaagac
      361 tgccatatcc tattgaccag gccagtagtg cgcctaatgt tttcaaaaca ttcttcagag
      421 cattaagccc ttacgactac acactgtatt gcaggaagtg ggtaaaacca aatctgactt
      481 gtacgccaca ggatgatccc cagtatccag ggatggatac aaagacagca agtgattacc
      541 tgccacagac aaattggcca acaactcaat taccaccagg atatacttgt gcagaaccct
      601 atttattccc agacattaat aaacccgatg ggccagcaac agcagggata gatgatttgg
      661 gtgaaatttt agctaaaatg aaacagagaa catcgagagg aataagaaaa tgaaaagagt
      721 tttgttcttt ttgctgatga tatttgttag ttttggtgtt atagctgatt gcgaaataca
      781 agctaaagat catgattgtt ttactatttt cgctaagggt actatttttt ccgcgtttcc
      841 tgtcttaaat aataaagcca tgtggaaatg gtatcagaat gaggacatcg gtgagtatta
      901 ttggcaaaca gaactgggta catgtaaaaa taataaattc acaccaagcg gggcgagact
      961 cttaattcgc gtcgggtcac tacgccttaa tgaaaatcat gccatcaaag gaacgttgca
     1021 ggagttgata aatacagcag aaaaaacggc gtttctcggc gatcggttta gaagttatat
     1081 aagagcgggt atctatcaaa aaaagagcag tgatccagct cagttgttgg cagtccttga
     1141 taattcaatc atggttaaat attttaaaga tgagaagcca acttatgctc gaatgacagc
     1201 acatctcccg aacaaagatg agtcttacga atgtttaaca aaaatacagc atgaactgct
     1261 tcgtagtgaa gaaaaataaa acatcttcct gagagaataa attaaccatt cggaaaaaca
     1321 tgttcttaat ggatatccag gtacaccaga atgtcgattt ggaaaaatgg attccgatgg
     1381 taagaatatc atatattctg aatgtaatgt tttacccaca gcttttgaga ttcgataggt
     1441 gtagaacatg agaaactcac taatattact tattatgata ttgtttacaa tatcataaca
     1501 agtatttgga aaagaaaatc cagtggaaat tttagtaaat agtcgtgaaa tggctttgga
     1561 attatcatat gtttacatta aatatgtata cgggaaagaa aaagcagaat ttcagaaacc
     1621 atatagtatt acagatgaca ataattgttg gaaaatcgag ggaaaacaac cgaagacttt
     1681 aggaggaaat tttacaatac taatagcgaa aaaagatggg caagtgttga atgtaataca
     1741 cacaaaataa tccactgtac agtcgattca aatattttac caaggtgtgt ttcgtaacaa
     1801 ttttgtgtac aatatgactc tacatatcga cgaaaaaacc acttgatttg acaggaacac
     1861 attcaaaaaa atttcttttt tcatttcagg agcgattttc atctgttact tgaagtatct
     1921 gtgagattaa gtgactcccc ctaacgtcgt actaaatagt aattactggc cataccggga
     1981 gcagtattcg gcgctcccca catgcagcag aataaccgca tcagcgaaga tgtattgtat
     2041 ttttactatc acaatgtgta cggacggttt atggaaaaga atgaacatta gatacgcgat
     2101 gcggcggcag gttacacggt tatcactacg atagccaata ctggttggtg cattaccgcc
     2161 gtgaacaata ggacgccgta ctgctggaaa tttcttacct ttatgactcc ccgcgccgcc
     2221 gtatcgggaa acgggaagca atcacaggga aagatcatca gaagttcact ttttgtacta
     2281 aataagtcgc attttatgtt taaaaattga gatattcctc attacctgaa gctgtttttt
     2341 attgcttata catgatcaaa tactccttac ataattaagg agaacaaaat ggaacttaaa
     2401 aaattgatgg aacatatttc tattatccct gattacagac aagcctggaa agtggagcat
     2461 aaattatcgg atattctact gttgactatt tgtgccgtta tttctggtgc agaaggctgg
     2521 gaagatatag aggattttgg ggaaacacat ctcgattttt tgaagcaata tggtgatttt
     2581 gaaaatggta ttcctgttca cgataccatt gccagagttg tatcctgtat cagtcctgca
     2641 aaatttcacg agcgctttat taactggatg cgtgactgcc attcttcaga tgataaagac
     2701 gtcattgcaa ttgatggaaa aacgctccgg cactcttatg acaagagtcg ccgcagggga
     2761 gcgattcatg tcattagtgc gttctcaaca atgcacagtc tggtcatcgg acagatcaag
     2821 acggatgaga aatctaatga gattacagct atcccagaac ttcttaacat gctggatatt
     2881 aaaggaaaaa tcatcacaac tgatgcgatg ggttgccaga aagatattgc agagaagata
     2941 caaaaacagg gaggtgatta tttattcgcg gtaaaaggaa accaggggcg gctaaataaa
     3001 gcctttgagg aaaaatttcc gctgaaagaa ttaaataatc cagagcatga cagttacgca
     3061 atgag
//
LOCUS       DAZGCQ010000246         3033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000246 DAZGCQ010000000
VERSION     DAZGCQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.249"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..244
                     /locus_tag="ACHK07_005183"
     CDS             <1..244
                     /locus_tag="ACHK07_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105361.1"
                     /translation="AHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPGELHYVIDAELK
                     DAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            297..602
                     /locus_tag="ACHK07_005184"
     CDS             297..602
                     /locus_tag="ACHK07_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105362.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            657..971
                     /locus_tag="ACHK07_005185"
                     /pseudo
     CDS             657..971
                     /locus_tag="ACHK07_005185"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            1296..1445
                     /locus_tag="ACHK07_005186"
                     /pseudo
     CDS             1296..1445
                     /locus_tag="ACHK07_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1620..2021
                     /locus_tag="ACHK07_005187"
                     /pseudo
     CDS             1620..2021
                     /locus_tag="ACHK07_005187"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            2037..2669
                     /locus_tag="ACHK07_005188"
     CDS             2037..2669
                     /locus_tag="ACHK07_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO8105363.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIVRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          811 a          696 c          820 g          706 t
ORIGIN      
        1 ggcgcatcaa ctgatgcgtg tgctttcaga tgttctgctg aagtatgagc cacgtattaa
       61 gcgtgtcaac gtgacgatgc aggagcagac tcagcctggt gagttgcatt atgtcattga
      121 tgcagaactt aaagatgcag gactggttcg ttacggaaca acttttatgc cggagggcag
      181 ggtgttactg cgatatctga agcaacagca gtttgtggat aatgtgatga ctttcagggg
      241 gtgatttatg gttcccctta tccgtgtgct taacagacat aaacgagggc tgaatgatga
      301 acccgttatt taatgacatc cagatgcgtc ttttttatct gaaccactct ccgtactcct
      361 ggcactggaa tgtcagattc agaccccagg aagctgtcta tattggcagc gatacctgtc
      421 atatcaccat tacctgtaat cagtcaggtt ttcatttaac ccgggacgga caacggctgt
      481 tcactgagcg ttatatcaga aaccttaatg agctgttacc ggttctgaaa cggcggtggg
      541 atgtgactcc ggcaattatc cgcgcggtgg aatacctttc ccgggtaccg gtatcacact
      601 gatgtccttt ccgtaactgt taatcatgat ttaatacagc aacaccgaat ctgccgatga
      661 cttctgaagt gaaactgaaa accggtggtg acccccggtc attaccggat tatgctgccc
      721 tgcgtgatga aatcagcaaa ctgacccacc cggcacgtcc ggatgtggac tggcgttacg
      781 tggaaacgct ctgcctgcgt ctgtatgaac acaatggcgt ggaattacag acggtatcct
      841 ggtacaccat tgcccggatg catacagcag ggttaagtgg tctgaatgaa gggctggctc
      901 tgattgtggc gctgacccgg catcactggt cagtgatgtg gccgctgaat acccacgccc
      961 ggctggaaat tattaccggt ctgttcagtc gtctgcaaaa aacgctccgg gccatgccac
     1021 cggatgagcg tgacaacctg cccctgctgt atcagacaga aacatgcctg aaaacgctga
     1081 gtgacatact ggcccggcat gaacttaaac agagcagtaa agtggcttta ccggaagcca
     1141 tggtgaaggg atatatcacc cgtctggaga atcagcctgt gcagggtgaa gtatcgtcgc
     1201 cggtaaccct tccggctcag gcactccggt ctgatgctcc ggatgtacag gaacatcagc
     1261 ccctgccgta ctcccgtctg gtgtatgttg tttctgaaac aaaagcagag cccacactct
     1321 cccttcagaa atccccgccc acgtttctga aaccgtttgt ggctggtgtc tgtgcggcat
     1381 tgctggctgt cagcgtggtc atcccgggat ggcagtttct gacacagcca tcaccggcgg
     1441 agcagcaact cagggcgctg ctggcaccac taccggatgt gctttcagca gaccagatgg
     1501 cgcaactcag cagaacaccc tcactgctaa atcatacgtc agagtggata accctttccg
     1561 gtcagcagat aacgcgactg acagaactac cgccggcata caacctgcaa cgcagtgcgc
     1621 agctgctgca gcaactgagg ggacttttcc ccgacaatcc ccgtgttcag gaaatggtgg
     1681 ataactggca taagagcgta aggagccgcg cgctcccgga agaggcgatg acgggctgga
     1741 acgaaggcat ggcccgttta cagcaactgg ctgagcgcct gaaccgtctg gatgaacagc
     1801 ggggaaaata tatgacggtc agtgaactga aaacggaggt gtttggcatc atgcaggctt
     1861 ttaaccggca cataccggca gaagagcagt tacgtcgtta tgatgaagcc cgtaaccaga
     1921 atggcagtga acagcagcaa aaacaggctg aaatggcgct gaatcagttg attaatcgtt
     1981 atcagatgga gaacgcaggc aaaccgaaga gccagccata atggctgaga taaaacatgg
     2041 aaatggaaaa tacccccgga agagcgcgca caccatggac aatcgaagat gttcgctacc
     2101 ttgaaacgca ttatggcgtg aagaaaaccc gcgaaatagc ggcatacctg aacagaagtg
     2161 cgacagcagt tcgtgccatg gcgtatctgg tggggataac aacaccagtg gctgcggact
     2221 ggtcagagac agagattgag attgttcgca gggactatcc ggcaggcgtt gcgcttgctg
     2281 atatttgtgc ccgtctgaac ggacgagcgc cgaaatccgt gtgctggatg gcccgcaggt
     2341 ttggtctgaa acgcctgggt tactggagtg cagaagaaga tgaaatcctg cggcaatatt
     2401 atccttcaga aggtgtgaaa gtgagccgac gcctttccgg acgtacagaa aatgcagtga
     2461 aactgagagc gcgggagctg ggtgtaaagt tcacgggaaa cgggcgatta cgtctctgga
     2521 cggcagagga gtggaatgtg ttgtccggaa atatggcatc ggggctatcg gcactgatga
     2581 agttactgcc ggggcgttcc cgtagttcag ttcagggggc tatgaggcgt ttacagaaaa
     2641 tgagtgcgca gaaatctgtc aggcagtagc caaaatattc ctgcatcagg agaacggcag
     2701 tggcaataat aagcagcaaa ctgatacata atgcagcttc tgtaaacggt aatgatcaag
     2761 tagggatatt tttatgttga ctggttgtga aaaaatgatc aaaatttaaa ttaacctttg
     2821 catgaaatta gaatctatta attttatcaa aggagattgt gtgacaatag taagagattc
     2881 tgttagtggt gaaattgttc atagtcatga tcttcgggaa atggatgttc tttatataca
     2941 aaaaaccact tttgaatgtc cgtatgacaa atgtaggata cctgctactc catgtagctt
     3001 caaagaaata aatttaaatc aatcatactt tcg
//
LOCUS       DAZGCQ010000247         2967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000247 DAZGCQ010000000
VERSION     DAZGCQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.250"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            285..2078
                     /locus_tag="ACHK07_005189"
     CDS             285..2078
                     /locus_tag="ACHK07_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105364.1"
                     /translation="MEKVTTKKINRTEHFPSLTLPSDTDWVVWWYGSLKKHKRRGEQP
                     KITVVFREIQTDGTLCDRYYEQECLLTDLSFFKIGSVWARGRCIAEILMEEKVFNVDF
                     SYANGGWKINSLYHAYQEGVSLPYDFSDYKLKSPYDKNWLLEFSTDGTAKIIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTQKVIERIQRQLDVCEDRDIYLKVAPWFKGKARIKVKGFWLGNNITFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCVLSPLRRKKAKYTSATKNESDLHSLYSVNEVVDEHNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLREAYSERVKSLDWYTPENSFLNTQEP
                     ELIAIPEFNESEAENDKISRSIINWPYLDAVNKKDIRGFLISRMLIDDNVIYFMEIQR
                     RVCNEETMNSVSEVEKFKGLVFTLDNECEIDKWVSLLAHESRFVKGILQKIVGKCPGT
                     AMTYKHSPAKNEPVACYSALLNALSKTGVTF"
     gene            complement(2068..2349)
                     /locus_tag="ACHK07_005190"
     CDS             complement(2068..2349)
                     /locus_tag="ACHK07_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105365.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(2407..2799)
                     /locus_tag="ACHK07_005191"
     CDS             complement(2407..2799)
                     /locus_tag="ACHK07_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105366.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCVMVPRISLPSL
                     KLTPLQSEIIIRQLLNGVNYIRHDGDVQGYEEKNVATFTIQEEGHHRFKADLSQNGES
                     DIASNVLNHWLHRIHKTDIIVLSVVLTE"
BASE COUNT          905 a          447 c          631 g          984 t
ORIGIN      
        1 gagtaataaa atgttagctg cgcgttgggg atgttcagaa gtatgggtga gtaaaattat
       61 taatgatgaa aatcgaaaag ttcagtggaa tgatgctatt aatgggcttc ctgttataag
      121 tgataatatg gtttgatttg cagttgtgtt ttgcctgcat aaggcgagaa aattactaat
      181 aattcattct ggatgcagtt cgtatcagaa cgttttttga agtgaataat tgttttaatg
      241 tctgcttgta atataagaac tatattgtat tgaggagtag atgtatggaa aaagtaacaa
      301 cgaaaaaaat aaacaggaca gaacatttcc cttcattaac gcttccgtct gatacagact
      361 gggttgtttg gtggtatggt tctttaaaaa aacataaaag acgaggtgag caacctaaaa
      421 taacagttgt ctttcgggag attcaaaccg atggaactct ttgtgacaga tattatgagc
      481 aggaatgtct gttgactgat ttgtcttttt ttaaaatagg cagtgtctgg gcaagaggac
      541 ggtgtattgc tgaaattctg atggaggaaa aagtatttaa tgtggatttc tcttatgcaa
      601 atggtgggtg gaagattaat tctctttacc atgcatatca agaaggtgtg tcattacctt
      661 atgatttttc agattataaa ctgaagtctc catatgataa aaactggctc cttgaatttt
      721 caacagatgg tacggctaag ataatcatcc atccgcttga gtttttttat cgttgctatg
      781 gacattctga tgaactgaag agggttattc ttacttattc aggaaatgaa ctacgtaaca
      841 ggattttttc tgaggcatac gtggagggag acaagatttt ttatataaaa ttaagaaaga
      901 gaatgaataa atatgatgct ccctttgcat ctttctttgc ccttgatggg tttacccaga
      961 aagtgattga aagaatacaa cgtcaattag atgtatgtga agatcgtgac atatacctca
     1021 aagtagctcc ctggtttaaa ggaaaggcca gaattaaagt caaaggtttc tggctgggaa
     1081 ataatattac cttcctgggg ctttctgtaa caggtgtttc tttgccagca ggatatacta
     1141 taaattatga cagagataac agtaatctgg ttgatgaaaa agctgaacaa gggagcaggc
     1201 aagcctggag taatgttccc gttcggcata taaaaaatcc ggatgaacct ttcagtgttg
     1261 ataacgcttt ttcgcctgat tctggagcgt cgactcttga agttgaagcg tcagtttttg
     1321 agtttcttga cgatggctgt gtattgagtc ctttaagaag aaaaaaagca aaatatacat
     1381 ctgctacaaa aaatgaaagt gatttacatt cattatattc agtaaatgaa gttgttgatg
     1441 aacacaatag cataagcaca ggatatatgt cattagctga taaaaatgaa cccgaaagat
     1501 tatatatgga gtcaaaagga attgttcgtg acatgtggaa tgctatggtt tatttgaggg
     1561 aagcttattc tgagagggtt aagtctctgg actggtatac accagaaaac tcattcttaa
     1621 atacgcaaga acctgaactt attgcaattc cagagttcaa tgaaagcgag gcggaaaatg
     1681 ataaaatttc caggtcgatt attaactggc cttatcttga tgctgtgaat aagaaagata
     1741 taagaggttt tttgatatca aggatgttga ttgatgataa tgtgatttat tttatggaaa
     1801 tacagaggag agtgtgcaat gaagaaacta tgaattcggt ctctgaagtg gaaaaattta
     1861 aaggtctggt ttttacactt gataatgaat gtgaaatcga taaatgggta tctttgttgg
     1921 ctcacgaatc aagatttgtt aaaggtattt tacaaaaaat agtggggaaa tgcccgggca
     1981 cagcgatgac ttacaagcat tctcccgcaa aaaatgaacc cgttgcttgt tatagcgctt
     2041 tgcttaatgc attgtctaaa actggagtta ctttttaata attctgttaa tggttattag
     2101 ctcacctaat atttcgaagt tatcaggtgg gcagttgatc catttgttag agccagcgat
     2161 gatttccctg tcccagcgtt ttattctttc gccatttttt tctatgcata tcatgtaatt
     2221 tgatggggtc attaattctt tggatactga aagacattcc cgaaatgatg gtatttcaag
     2281 caaaacttcc gcgcctgaat tttcacggta aaaaatgtgc catgttgtgc cttttttatt
     2341 agttttcacg tgcgtattct cataggataa atataaaacc ttgtaacatt caggaggtaa
     2401 ctggatttat tctgtaagaa caacagacag aacaatgata tctgttttat gtatacgatg
     2461 tagccagtga tttagtacat tgctggctat atcactttcc ccattttgtg ataagtctgc
     2521 tttgaaacga tggtgtccct cctcctgtat tgtgaaagtt gccacatttt tttcttcata
     2581 tccctgaaca tccccgtcgt gccttatata attgactcca ttaagaagtt gtcgaataat
     2641 tatttctgat tgcaacggag ttaatttcag tgatggtaaa gatatccgtg gcaccataac
     2701 gcatttttca tcatcagtat agtggtatgc gtgaacatat atggtggcat tttgcctgat
     2761 gttgttaagg cttttcagca ggtctacagt caactccatc gaggtatcct tcaggttgtt
     2821 atcattcgtt gatgatgcga tctgttgaag gcgttagcga caattaattc tttttctctt
     2881 ctgaaaaatt tacggaatac aaaaaagatc tgtttttaac tgattgttac tttctgaaga
     2941 tattctctgc tccatttgct ttccggg
//
LOCUS       DAZGCQ010000248         2960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000248 DAZGCQ010000000
VERSION     DAZGCQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.251"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..615
                     /locus_tag="ACHK07_005192"
     CDS             <1..615
                     /locus_tag="ACHK07_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8105367.1"
                     /translation="GKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQ
                     NPRLFLGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGL
                     SGGQRQMVALTRMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRL
                     QVLNIVDRIVVMNEGRVMMDGPRDEILKKLSGKQQSEESRQAAS"
     gene            675..1922
                     /locus_tag="ACHK07_005193"
     CDS             675..1922
                     /locus_tag="ACHK07_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO8105368.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            2207..>2960
                     /locus_tag="ACHK07_005194"
     CDS             2207..>2960
                     /locus_tag="ACHK07_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105369.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGE
                     QKPVQPEGGQEAGKTDVSSTGSTEAGSGSSSEQVPEVNDQNSNGKPDAQDAKDAEDAA
                     KALVEAAEAKQAEAEGALTEANKDGAINPDEHT"
BASE COUNT          814 a          578 c          904 g          664 t
ORIGIN      
        1 ggtaaatcga cgctgctgaa aatgatggtg gggctgtacc gtcagaacag gggacaaatt
       61 actcttgacg gggttgcgat gcagcaactg gacccctgtt ttgtccggga taaagtgttt
      121 cttctggaac agaatccccg gctttttctg ggaactctgc gtgaaaatct gcagctttcg
      181 cgtctgccgg gaagcctgtc ggatgtggat atgattaatg cagccggacg tttcgggttt
      241 gaccgtttta tccggcatca tccccgcgga cttgatatgc agacagggga aggcggtcag
      301 ggactgtcag gaggacagag gcagatggtt gccctgacga gaatggtact gggttctccc
      361 cgggtggtac tgcttgatga gcccacgaca ggtctggacc tgcagactga gtctgttgtt
      421 ctgaaaggtc tgagccactg gagccgtgga cgcacaatgg tggtggtgac acatcgtctg
      481 caggttctga atattgttga ccgtattgtc gtgatgaatg aagggcgggt aatgatggac
      541 ggtccccgtg atgaaatact gaaaaaactg tcagggaaac agcagagtga agagagcaga
      601 caggcagctt cctgaacgtc aaccggataa gaaaaaagag cagatatgtt taacagtttg
      661 tggaccgcag ggatatgaca gaaaaaacgg aacggaataa agcgtcgtca gaggtggtca
      721 ggcagtcaga cctggcgctg ttgacagatg tacaggcagc ccttcagcag gaaaagcatc
      781 atgccctttt cctgatggtg atttttctgc tggtactggt agtgacgttt gttatctggg
      841 catggaacag tcctctggat gaagtgaccc gtggtcaggg gagtattatt cccggcagca
      901 gggagcaggt tatacagacc cttgacccgg gtatactgaa atcacttgag gtcagggaag
      961 gcgatattgt tgagaaaggt caggtactgc tgacactgga tgatacccgg agctctgcca
     1021 tgctgaggga aagtgaagcc cgggtgaata acctggaggc cgtcagggca agactccgtg
     1081 cagaagctta ttccgaatca ctgacatttc cggataacgt tcctgaggat ttgcgggagc
     1141 gggaaagtac ggtctatttt ctccggaaga cggagctggc gcagagcatt gccggtctga
     1201 agcagagtaa ggcgctgctg gacagggaaa tagcaatgac gcggccaatt gtgcgtgaag
     1261 gggcaatgtc ggaggtcgaa ctgttacgta tgcagcgtca gtcagcggag ctgcagttac
     1321 agatggatga gaaacagaat aaatacctga cggaagccgg tgctgaactg gtgaagacag
     1381 aagcagagct tgctcaggca aaagagaata tggctggcag agccgacccg gttgagcgtt
     1441 cacgtctcag ggcccccctg agaggtatcg taaaaaatat acgggtaaac acactgggag
     1501 gggtggtttc tgcaggacag gacataatgg aaattattcc gctggaagac cagttgctga
     1561 ttgaagctta tataaatcca cgggatgttg cctatgtcag aacagggatg ccggcacttg
     1621 taaaactgac ggcttatgac tatgccattt atggtggtct tgacggcgtg gtgacactgg
     1681 taagtccgga tactttacgt gaccagaaac ggccgggaga tttaaaactg gacccgaatg
     1741 aagcttatta ccgggtactg gttaccacca gtaataatta tctgacagac aggaacggaa
     1801 aaagattgcc agttattcct ggcatgatag ccagcgtgga tataaaaacc gggcgtaaaa
     1861 cggtatttca gtatctgatt aaaccgataa ccagaatgaa gcaggcactt caggaaagat
     1921 aataaaaagg tcttccgggg aaagtatttt aaatcaattt taaacgggca gaatgatttc
     1981 actttattct gcccgggtca ttgcgcatgc ctgtatgtga tttggggctg aatataatat
     2041 aaatgttata tctgtcaggg gaaaataaat ctgaaaaagc ggaatgatta aggaggtccg
     2101 gccagagaaa taaattatct gtaatctgat tcgtttttat ttaatgactc cctttagcgg
     2161 ataaaacata agagcatact ggttttatta acctgaggat acttatatga aaatgatttt
     2221 caccggcaag gtcagtggag aaaaaacagt actgacagcc ggagcccgtc atactgtaaa
     2281 ggcacaggcc ggggaacagt atggactggt tgatgaggtg acaggtctgg tcccggatgg
     2341 tgtggaagcg gaccgttctg gtgatgattt aattctgcgt aaaaaggaag atgatacaga
     2401 aatccggatt gagggattct gggaagaatg ccagcctggt gaaacacagt gtacagccgt
     2461 gtttaatgtt gtgggtgaaa atggccaggt aactgaagct gtcctgaccc aggatggtcc
     2521 tgtactggag aattttactg cagggcaaag tggcacactg gctgaaggta tcaataacgg
     2581 gattatctgg ggggggattg cttttggtgc agggctggcg gctattgcac tggctgccgg
     2641 aggtggtggt ggaagcagtc gtcataagcg tgcaactgaa ggtgagcaga agccggttca
     2701 gcctgaaggc ggtcaggagg ctggaaaaac agatgtatca tccacaggct caactgaggc
     2761 gggaagtgga agctcttccg agcaggtacc ggaagtgaac gatcagaaca gcaacggcaa
     2821 gccggatgca caggatgcga aggacgcaga agatgccgcg aaggcgctgg ttgaggcagc
     2881 cgaagcgaag caggctgaag cggaaggtgc gctgacggag gccaataaag acggagccat
     2941 taatccggat gagcataccg
//
LOCUS       DAZGCQ010000249         2936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000249 DAZGCQ010000000
VERSION     DAZGCQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.252"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(528..1619)
                     /locus_tag="ACHK07_005195"
     CDS             complement(528..1619)
                     /locus_tag="ACHK07_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8105370.1"
                     /translation="MITCVFIFTAILLAVITFIPVSGNTSWVVRVWDFPRLQILLLCT
                     LNVFLSVVFLDSFAELFVVIISLICVAYQLVWIIPYTVIGKKQVKEIKDSDGFPGLSI
                     LVFNVLMTNKKYMPLLELINKEKPDILVLLETNLWWEQKLSPLDEVFPYSLKCPKENL
                     YGMHVYSRMILSDAEIQYLVDKEIPSMHMIVHMPEGQRIRLHCLHPVPPSPTESDEST
                     DRDSELLMVGKSVADSELPVILAGDLNDVAWSATTRLFMKISGLLDPRRGRGFFSTFH
                     SHYFFLRWPLDHVFHSQHFLLKSLRRLPDMGSDHFPVMAELVYAPAHGMQQESLEKEK
                     SDEVLAQKKMDMTDSTAEDVHTPGGQKKS"
     gene            complement(1882..2070)
                     /locus_tag="ACHK07_005196"
     CDS             complement(1882..2070)
                     /locus_tag="ACHK07_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8105371.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            complement(2145..2621)
                     /locus_tag="ACHK07_005197"
     CDS             complement(2145..2621)
                     /locus_tag="ACHK07_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8105372.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEFVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            complement(2784..2879)
                     /locus_tag="ACHK07_005198"
     CDS             complement(2784..2879)
                     /locus_tag="ACHK07_005198"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105373.1"
                     /translation="MKTVCAHLYNAMEKSGLRGVSVKYLCSTTDR"
BASE COUNT          895 a          715 c          531 g          795 t
ORIGIN      
        1 tagcgtaatg gagtgttatg tagcatggtg ccgggtgcct cccggtgaac agactgttcg
       61 tgataccagt cagcaacata ggaaattaat ggtatcaccc caccgcacag ggggattcac
      121 catgcctgcc ttttttagca aattcatcag aaaccggaca acatgcttat ttatgaaatg
      181 aggtgctttt cacatttata taaaatcatt ataacgataa tcctaaaaat caatgtaatt
      241 atcatcttca gtaactttct gtattacacc atacggaaat tgatttttga cggtaaagat
      301 acaaggggaa atatgtctgt aaggagtacc ctgaataatg gacaggcatg catcgtggct
      361 gttcccctga aattatcagt acaaccagtc agctcataat ccctgtctct ttttccgggg
      421 agccctcagg gggactttcc cttatccccc cagtttctgg agagacgtaa aaaagaatta
      481 atcatatgtc aggtatctga atatgtcccg taattaatat atttacgtca ggattttttc
      541 tgcccgccag gagtatgaac atcttccgcc gttgagtctg tcatatccat ttttttctgt
      601 gccagtactt catctgattt ttctttttcc agactctcct gctgcatccc gtgagccgga
      661 gcatatacaa gctcagccat cactggaaaa tgatctgaac ccatatccgg taaacggcgt
      721 aaacttttaa gcagaaaatg ctggctgtga aatacatggt caagcggcca tcgcaggaaa
      781 aaataatggc tgtgaaacgt gctaaaaaaa ccacgtcccc tgcgtggatc cagtagtcca
      841 cttattttca tgaataaacg agtggtcgca gaccacgcaa catcattcag atcccccgct
      901 aatattacag gtaattcaga atcagcaaca cttttcccga ccattaaaag ttctgagtca
      961 cgatcagttg actcatcact ttcagtcgga ctcggtggaa caggatggag acaatgtaat
     1021 cttattctct gaccttccgg catatgtact atcatatgca tggagggaat ttccttgtcc
     1081 actaaatact gtatttctgc atcacttaaa atcatccttg aatatacatg catgccatat
     1141 aaattttctt tcgggcattt cagggagtat ggaaacactt catccagagg tgatagtttc
     1201 tgttcccacc ataagtttgt ttcaagaaga acaagtatat caggtttttc tttatttata
     1261 agttcaagca aaggcatata ttttttattg gtcataagga cattgaatac cagtatactt
     1321 aaacccggaa atccatcaga gtcctttatt tcctttacct gcttcttgcc aattactgta
     1381 taaggaatta tccataccaa ctggtaagcg acacaaatta aacttattat cacaacaaac
     1441 aactctgcaa atgagtcaag aaaaacaaca gacagaaaaa cattcaaagt acacagcaaa
     1501 agtatctgta gtcggggaaa atcccatacc ctgacaaccc atgatgtatt accggaaaca
     1561 gggataaaag ttatgactgc cagaaggata gcagtaaaaa taaaaacaca agttatcaca
     1621 aatcactcct tgttctgaac cggcacacaa aactgtcata tacgtttcaa aagtaaaaac
     1681 acactgctgc cacaagattc acgacgtaac cgggcagcat atcctgatta cggacaatcc
     1741 atgaaaccgc ctcgccagaa gcgtccatca catccggttt ttccctgttt tatattccac
     1801 gaaccatttt attttcagga atctccgggc ctttatcccg catcatatac acagctatga
     1861 caacccgctc ttctgcagtc atcatttccc cgtggcttcg cggattttct gttccgtctt
     1921 atactgcgaa agcgcataca cggcccatat tgttgccggt atccagccca gcagcgttat
     1981 ctgtaacacc agacaaaaaa tccccgtcag aggccggccg atggtgaaaa aagtcagcca
     2041 gggaagaaga aaagcaatca acagacgcat aaaaactcct tatgattatg aaattaacga
     2101 atcagaggct gcgtttaaca ttaccggaaa taagctgtcc tgcttcacaa cctccctttt
     2161 ctgaccagaa gcatcccctt gtacaacaac tcaacttcat tatgtacccg caacctcttt
     2221 atcagattct gcttatgagt ggagaatgtt ttctctgaca tgccaagata tcgtgccccc
     2281 tctgttccgt tactgccata aagcagcatc atcagtacat ttacctgccg gcctgacagg
     2341 gtcagcttct ccaccgacat gaatgcgcgg tacagatgac tgatatcgtt tactgaacat
     2401 cgctcagtaa gatgtctgat atcataaacc ggactgtcaa ccactcccca gagaaaacgg
     2461 agcgcttccg gagaagaaaa ataaagggtg ttcgtatcac ttatattatt cttatacaga
     2521 aaaccgacaa attcagatat ccgggaagaa gatattatca ccacattatc tgtctgcatg
     2581 ccgctgataa tgaatttacc tggattaata tactccttca ttccctccag aaaataactg
     2641 ttatcacaaa taaaacgcat acccgttcca catgaaattt atcacgcctg accacataaa
     2701 attaattatc cggagcaccg ggatgcccgt gacacagcat gcacagaaat tcccgtcgca
     2761 acagccttca tcagaccggc tttttaccgg tctgtggtgc tgcagagata tttaacactg
     2821 acacctcgca gaccgctttt ctccatcgca ttgtaaagat gcgcacatac cgtcttcatg
     2881 cttatcccca actggtctgc cagaaaggta aacccgtact catggaaaac aacccc
//
LOCUS       DAZGCQ010000250         2851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000250 DAZGCQ010000000
VERSION     DAZGCQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.253"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..2429
                     /gene="ydeP"
                     /locus_tag="ACHK07_005199"
     CDS             150..2429
                     /gene="ydeP"
                     /locus_tag="ACHK07_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO8105374.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICSGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2677..>2851
                     /gene="safA"
                     /locus_tag="ACHK07_005200"
     CDS             2677..>2851
                     /gene="safA"
                     /locus_tag="ACHK07_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO8105375.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          770 a          660 c          691 g          730 t
ORIGIN      
        1 taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc ctactcttat
       61 atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct
      121 aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggag
      181 ctgccggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata
      241 tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg
      301 gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      361 aagcaatcgc ctgggaagtc acggataagc aggtaaacgc ctctttcttt gctgagaata
      421 cggttcaatc attacttacc tggggagacc acgagcttga ggctgcgggg cgactcactc
      481 agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt
      541 tcgatgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      601 cttcgggccg cacttccaat gaggctgcct ttctttatca gctttttgcc cgtgaatacg
      661 ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg
      721 cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt
      781 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc
      841 gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc
      901 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
      961 tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga
     1021 tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgctgg
     1081 atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca
     1141 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1201 tggctgatgc atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc
     1261 agcacgaaca tggtacccag aatgtacagc aactggtcaa tctgctgttg atgaaaggta
     1321 acattggcaa gcctggtgcg ggtatctgcc cactacgtgg ccactctaat gtacagggcg
     1381 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc
     1441 gctatggctt cacaccacct caagcacctg gacatgctgc aattgccagc atgcaagcaa
     1501 tatgttcagg gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc
     1561 cagatcggga agcgagcgct gtaccgttaa cgcaattaga tctggcggta cacgtagcca
     1621 ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg
     1681 gacgtagcga gattgacatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa
     1741 tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag
     1801 agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg
     1861 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1921 tcgccgatta taaccagcgc atccgacatc ctggtggttt tcacctgata aacgctgctg
     1981 ctgaaaggcg ctggatgact cagtcaggta aggctaattt cattaccagc aaagggctgt
     2041 tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg
     2101 atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac
     2161 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2221 gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat
     2281 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga
     2341 atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc tataaaagta
     2401 ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg ctgtcatccg
     2461 cacagcagaa gaatacctca ttgactatta tcgcgcaatt tgctcacatg gattaaatta
     2521 aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact
     2581 gaagtaccag tcctatttcc tcttttctca agcctgttat attaagcata ctgattaacg
     2641 attttcaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac
     2701 aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt tgtcttatta
     2761 ctgaccctta cgttgatagc cattttttcg gcaattgatc agctgggtat ttcagaaatg
     2821 ggtcgcattg caagagatct tacacatttc a
//
LOCUS       DAZGCQ010000251         2845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000251 DAZGCQ010000000
VERSION     DAZGCQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.254"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1068)
                     /locus_tag="ACHK07_005201"
     CDS             complement(<1..1068)
                     /locus_tag="ACHK07_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO8105376.1"
                     /translation="MFPLNDLSLKTQSVQLNKVTSNTESTMKQQELVSDDAMINELSS
                     ELVSCLGNGKFTPISEGGKLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDFKE
                     NVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPEWIEMLSLVYNELDSIQVPESCKE
                     LEVDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKIISICHNKLSEIPALPDTA
                     KVFDCSENNIKEIRWFPKNLKEAYIEYNKIEVIPAIPGNLKLLFMECNPIKEAFLMPW
                     TLTDITYEISQRKYIVTNPDDY"
     gene            1350..1750
                     /locus_tag="ACHK07_005202"
                     /pseudo
     CDS             1350..1750
                     /locus_tag="ACHK07_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1905..>2845)
                     /locus_tag="ACHK07_005203"
     CDS             complement(1905..>2845)
                     /locus_tag="ACHK07_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO8105377.1"
                     /translation="DSKLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGAITIEK
                     SQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDVKENV
                     DRALLRMFKCVELHNNELDLSYLFWGSVPPLPEWIEMLYLSDNQLDSVQVPESCKELG
                     LDSNNLTEFPQVPDGITLISVNNNLISHIDSFPPKIKEIYIRHNKLSQIPEIPDTTTV
                     FDCGCNKIQEIRYFPKNLEKALIEYNNIEVVPAIHSKLKLLFIECNPIKEAFLMPWTL
                     TDITYGISQRKYIVTNPDDYDKYSYMVKRIVIDGHN"
BASE COUNT          847 a          589 c          507 g          902 t
ORIGIN      
        1 ataatcatcg gggttcgtaa caatatattt ccgctgcgat atttcatagg taatgtctgt
       61 caaagtccat ggcattaaaa atgcctcttt aataggatta cattccatga aaagtaattt
      121 taaattgcca ggtatcgcag gaataacctc aatcttatta tattcaatat acgcttcttt
      181 cagatttttg gggaaccatc taatttcttt aatattattc tcactacaat caaaaacctt
      241 agcggtgtct ggtagtgctg gtatttccga tagcttattg tgacaaatag aaattatctt
      301 agcctttggc ggaaatgagt caatatgcga tatcaggtta ttatttacgg agatcagggt
      361 aattccatca ggtacttgtg gaaattctgt aaggttattg aaatcgactt ctaattcttt
      421 gcatgattcg ggcacctgta ttgaatcaag ttcattataa acaaggctaa gcatttcaat
      481 ccactcagga agaggcggca cagaacccaa aaataggtat gataagtcaa gttcattact
      541 gtgaagctta acacatttaa acattctcac caatgctcta tccacatttt ctttgaagtc
      601 attcgatgcg ccttctttga ctgaactagc ccagtcactg aaacctttgc aaagtctggc
      661 atctgtaatt ttggttaact tgtccagcaa ctctgcaata ttggtcgata taaactctaa
      721 tttatgccga atgggtaaag tcccctcccc ctgacttttt tctatcgtga tagatcccgt
      781 cttatcataa gccccataat tttcaaacaa tagagtataa ttaccccacc tgaaacattg
      841 ctctctcaat aacttaaatt ctgacagcat attcaataat ttgccgcctt cactaattgg
      901 tgtaaactta ccatttccaa gacaactgac taactcactt gataattcat ttatcattgc
      961 atcatcagat actaactctt gttgtttcat cgtagactca gtattagatg tgactttatt
     1021 taattgaacc gactgagttt ttaacgaaag atcatttaat ggaaacattt acactcctgt
     1081 ttaataaatt atattgccca gggcgcattc taaaacacaa taaaattacc actatcaaaa
     1141 atcgctcata tccatcgccc ggaatatgaa taaaaacatc aacaaagatt aaattattca
     1201 aacgttactg ttgcgtcagt ggtcgtagca acatccgacg tggtgatgtg tgttaaagga
     1261 gacgctctga tgttcagggg ggttatcgcg tatacggatc gtggatggca gaaaacgttc
     1321 aggatcaggt atccatttta aaccagataa tgagtaagtt tgccccatcc atgccccacg
     1381 cggtgagatc ggatgtcatt aaaaaagtca ggtcagcagg tcagtatggg aaggcgaaca
     1441 actcgcagga atgggtgcgc tgaagttgct ggatgataaa catggcgagc tgaaatcaat
     1501 gcgtaccgcg ccaaattatt tacgtcgggg tgtcgccagt ctgattttac gccacatttt
     1561 gcaggtcgcc catgacagat gccttcatcg cctgagctta gaaacgggta cacaggctgg
     1621 atttacggcc tgccatcaac tttatttgaa acatggattc gttgattgcg aaccgtttgc
     1681 tgattatcga tttgatccac acagtcgatt tttatcattg acgttatgcg aaaataatga
     1741 attgccatga gcgagacgca gcacattctt gcattcgacg tgctgcgcct ttatttatca
     1801 ccagcaggaa acgccttgtc catagacgcc acttctacat gcgccacaag aaacctctat
     1861 tccagtgaca caattacgcc taattaatta cataaatgaa ttatttaatt atgaccatct
     1921 attactatcc ttttaaccat gtaagaatat ttatcataat catcgggatt cgtaacaata
     1981 tattttctct gcgatattcc ataggtaatg tctgtcaaag tccatggcat taaaaatgcc
     2041 tctttaatag gattgcattc tataaaaagt aatttcaact tgctatgtat cgcaggaaca
     2101 acctcaatgt tattatactc gataagtgct ttctccagat ttttggggaa gtatcgaatt
     2161 tcttgtatct tattacagcc acaatcaaaa accgttgtag tatctggtat ttctggtatt
     2221 tgcgatagct tattgtgccg aatataaatt tccttaatct tcggcggaaa tgagtcaata
     2281 tgcgatatca ggttattatt tacggagatc agggtaattc catcaggtac ttgtggaaat
     2341 tctgtaaggt tattggaatc gagtcctaat tctttgcatg attcgggcac ctgtactgag
     2401 tcaagttgat tatcactaag gtaaagcatt tcaatccact ctggaagagg cggtacagaa
     2461 ccccaaaata ggtatgataa gtcaagttca ttattgtgga gctcaacaca tttaaacatt
     2521 ctcagcaatg ccctatccac attttctttg acgtcattcg atgcgccttc tttgactgaa
     2581 ctagcccagt cactgaaacc tttgcaaagc ctggcatctg taattttggt taacttgtcc
     2641 agcaactctg caatattggt cgatataaac tctaatttat gtcgaatggg taaagtcccc
     2701 tccccctgac ttttttctat cgtgatagcc cccgtcttat cataagcccc ataattttca
     2761 aacaatagag tataattacc ccacctgaaa cattgctctc tcaataactt aaattctgac
     2821 agcatattca ataatttgct gtctt
//
LOCUS       DAZGCQ010000252         2821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000252 DAZGCQ010000000
VERSION     DAZGCQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.255"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..2821
                     /locus_tag="ACHK07_005204"
                     /pseudo
     CDS             208..2821
                     /locus_tag="ACHK07_005204"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
BASE COUNT          873 a          523 c          646 g          779 t
ORIGIN      
        1 atacagaaaa attcagtgca ggtgtataaa tcatcaccat gcgaaataca taaaacatat
       61 taatgtttgt tttccacagg aaaaatctta agtatggaca ggtattcctg atgccattta
      121 aaatagattc agggtgagtg ttcagcactc accctttccg gacggataat atattttaat
      181 actatataat tatataacag gaattatatg aataaaatat acgcgcttaa atacagctgc
      241 cttactggtg ggcttatagc tgtgtcagaa ttaagtaaga aagtttcggg aaaaaccgga
      301 agaagattaa tgacggtttc cctggtatta tcaatgattc tttctgcttt accgggtaaa
      361 gcatcaacgg tcagcgcaga aataccatat cagacttttc gtgactttgc tgaaaataaa
      421 ggtgtgttta cccccggagt gacaggaatt gaaataaaag ataataatgg aaatgcagtt
      481 ggtacgcttg atgtgccaat gattgacttc agcagtgtgt ctcggcgggg ctcactgacc
      541 ctgttgtcgc agggatatgg tgtatcagca aaacacggag gccttggtga tgtaaacaat
      601 gccagttttg gctatgataa aaataattat actgtagtaa agaacaacaa acattcgggc
      661 cttgattttt ctctgcatcg gttcagcaaa ctcatcacag aggctgcacc tgctgatata
      721 aatatatccg gacaacttag tgattcgtca cagtatactg ctttttacag agcaggcgct
      781 ggcacacagt atattaaaga acgctcaggt aaacagactc atatcccggg aacattttta
      841 actggtggaa cagtaggcac cccatggtat tcaggaaata atttaatatc aagctctcca
      901 ggtgacacct ataataaatc acagggacca ttagccagtt atgggcagat gggtgacagc
      961 gggtcgccat tatttgctta tgattctctc agtgaaaaat ggtctctggc tggagtcacc
     1021 ctgcataata atggtgttaa tggccaaaaa aaacaactgg ttgttattac ctgaagatta
     1081 tattaaaaat attattacag ccgatttcga tcccattatt tcatttaata aaaacagtaa
     1141 agaacatatg tcatggacat atgatgcagc caaaggcgtg ggccgtattc agcaggatga
     1201 ccaacagttt gtgatgcatg gtaacctgaa tggtaatctg aatgcaggta aaaatcttta
     1261 ttttacaggc gcaaatggca ttattgattt aaaagataat gttaatcagg gagccggata
     1321 tcttcagttt gcggatgatt atacagtaac aacgagcaat gccagttcat ggttcggagg
     1381 aggcattatt gtcaattcgg gtacaactgt aaaatggggt ataaacgggg tttccggaga
     1441 tgaccttcat aaagttgggg acggaacgct tattattaat ggcacaggga aaaatgaagg
     1501 gggactgaag attggggctg gtactgtaat ccttgaacaa aaagcgaaaa acaacgattc
     1561 aacggctttt tcttccatta acatcagtgg gggaaactcg cgcgttaaac tctcaggaga
     1621 taatcagatt atacctgata atgtttcctg gggattccgg ggtggatatt tagatataaa
     1681 cggaaaaaac acagagttct cccgcttaca ggctgttgat tatggggctg caattattaa
     1741 cagcagcaca gagaagtcat tattgacgtt aaatctgtca ccattgaaaa aagatgaaat
     1801 agctgtgagc gtgaaggcgt tagatatgaa cgccatcttt catggaggat acgggacaac
     1861 cggcgatttg tataaaacaa ctttttacgg tccgacacag tattatctgc tgaaaaagcc
     1921 caaatttggc agtgtgctga tggggtcact gaaaaacacc agcgaatggc aatttgcagg
     1981 aacagacctg aatcaggccg ttgatatggc aaagaataat aaactgacat caagtgcaca
     2041 ggcttcatat ctttatcacg gtaagctcct ggggaatatg gatattgtca ttccggagct
     2101 tacaggcaat gatattttaa cgcttgatgg ctctgtcagt atatcaggag atatgtcaaa
     2161 acaggacggt gctcttatct tccagggaca cccggttatt catgcagggc aaactgtttc
     2221 tgcatcgcag agtgactggg agaacaggga gttctcactc aacaatctga atcttaataa
     2281 tgcggacttc agtctgtccc gtaatgcatt tatgaacggg aatatcaggg ccgttaacca
     2341 gagcactgtt attatcggcg gagatacagt ctttactgat aaaaatgacg gaacaggtaa
     2401 tgatgtcatc agtgttgaag ggaaatctgc tgccgcagga acatcctcct atacagggca
     2461 tatcactctg gagcaaaaat cagcactgga tatccgcgat aattttcgtg gcggggttac
     2521 gtctgaagac agtcatatca atgtttcttc atcttcagtc ctgttctcag atgcatcgtc
     2581 atttataaac agctccctga atattcataa aggaggtgcg ctgaccgctc agggagggct
     2641 gtttacaagt ggaagcattg atattggtga cgcttccctt ctgcttaccg gtacaccagt
     2701 gaattcagat gatgctgctt ttttaccgac catcaatatg gctgatggcg gatttaaact
     2761 gatgtctgat tcatcagtac tgaaagccag agaccaggca tctgttgttg gtgatattat
     2821 t
//
LOCUS       DAZGCQ010000253         2754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000253 DAZGCQ010000000
VERSION     DAZGCQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.256"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..880
                     /locus_tag="ACHK07_005205"
     CDS             155..880
                     /locus_tag="ACHK07_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8105378.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            838..1515
                     /gene="bglJ"
                     /locus_tag="ACHK07_005206"
     CDS             838..1515
                     /gene="bglJ"
                     /locus_tag="ACHK07_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO8105379.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1553..2341)
                     /gene="fhuF"
                     /locus_tag="ACHK07_005207"
     CDS             complement(1553..2341)
                     /gene="fhuF"
                     /locus_tag="ACHK07_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO8105380.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2482..2718
                     /locus_tag="ACHK07_005208"
     CDS             2482..2718
                     /locus_tag="ACHK07_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO8105381.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLTYADLSPDKWRIVILMGLLLTP
                     LMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
BASE COUNT          736 a          591 c          696 g          731 t
ORIGIN      
        1 gcagtggagt tgttgtgcag caggagtatg ctgatatgaa agtgaatgct aaggataatt
       61 tattcgctta atctattaat ttgctggaaa catttaagga atgaatgtcc tgaataaacc
      121 atcataattt ttagacagtg gatgtggagg aaatatgttg ccaggatgct gcaaaaatgg
      181 aattgttatc agtaaaatac ctgttatgca agcagggtta aaagaggtca tgaggactca
      241 cttccctgaa tatgaaataa tatccagcgc ctctgcggag gaccttacct tattacaatt
      301 acgtcgttcc ggattagtca ttgctgattt agccggtgaa agtgaagatc cacgttctgt
      361 ttgtgaacat tattattctt taatctcaca atatcgggaa attcactggg ttttcatggt
      421 gtcacgctcc tggtattccc aggcagtaga actgctcatg tgccctacgg cgacgttatt
      481 gtctgatgtt gaacccattg agaatctggt caagaccgta cgttccggca atacgcacgc
      541 agagcgtatc agcgccatgc tgacctcccc ggcaatgact gaaactcatg attttagcta
      601 tcgctccgtc attctcactc tttcagagcg caaggtactg cggctattag gtaaaggatg
      661 gggcatcaac cagatagctt cattgcttaa gaaaagtaat aaaactatca gcgcccaaaa
      721 aaacagtgcg atgcgtcgac tggcaattca cagcaacgct gaaatgtatg catggataaa
      781 tagcgcgcag ggtgcaagag aacttaactt gccttctgtt tatggagatg ccgcagaatg
      841 gaacacagcc gaattaagaa gagaaatgtc gcactcatag aaaaatgcgt catgagtagt
      901 atcggtattg agagtttatt cagaaagttt gcgggcaacc cttataagct ccatacctat
      961 accagtcagg agtcatttca ggatgccatg tcgcggatct cgtttgcggc ggtcattttt
     1021 tctttttctg ccatgagaag tgagcgcaga gagggattat cttgcctgac tgaactggcg
     1081 attaagtttc cgcgtacccg gcgtttagtt attgcggatg atgatattga agctcggctg
     1141 attggttcat tgtcgccatc accgctggac ggtgtattaa gtaaagcgtc aacgctggag
     1201 atttttcatc aggaactctt tttgtcatta aatggtgtac gtcaggcgac cgaccgactg
     1261 aacaatcagt ggtacattaa ccaaagccgg acgttaagcc cgacggagag agaaatattg
     1321 cgctttatgt cgcgtggcta ctcaatgaca caaattgccg agcagcttaa acgcaatatt
     1381 aaaacgatcc gtgcacataa atttaatgtg atgtcgaaac tgggcgtcag ttctgacgca
     1441 gggttgttgg aggccgcaga tattctgtta tgtatgcggc attgcgaaac aagtaatgtg
     1501 ttgcatccat attaatccgc atgatgccgg gtttacttcc ccggcagtgc tttcatttca
     1561 gcgtacaatc gccacattgc tgcacatccg gtaagcgata acgctggcag caagtgcggc
     1621 gcaccagcag gccgtcgcgt agcaccacgg tacgccagag tggattatct tcaccggtag
     1681 taagcgtttt ctcaaaaaag agggcatggc gcagcgattc aaccgtcgcc tcgccgagca
     1741 gttgcttcat ctcagtaaga taccagttga tcaaataacc ggtattactc cagataagtt
     1801 tgccgttgat ctctccggtc gcttctagtg cctgcacaac cggaaccagc gcctggctga
     1861 ttaacgtttc cattcgatgc tgcggcgaat gtggtgttgc gtttttatct tcacatacat
     1921 cgacccagaa acaggcggcg cgtccggttt cgtgaaactc aacgtggaaa tgttccggcg
     1981 acacatctaa tgctttttcc tgcgtcagca gcgccagcat taatggcggc accatcaggc
     2041 cgatatacca ctgcgcccac agtgagatca gcggtttgtt ctcgcggatc atcatcggtt
     2101 ggttgcgata gatatgatcg gaatagaccg ccagcagaga acttagcaca ttcggtgatg
     2161 accattgcgc cagcgtcatg gcgttaagtg gggcaggttc atccaggcgg ataaactcga
     2221 gcaaatgttc acgatgtttt gcgatcgtcg cccgcacggc ttgcgcaagc gtgggatcct
     2281 gcggctggag atgcgttcgc cagataacat cttcatagag cggtgcggaa cgataggcca
     2341 taatcgggat agtaatctaa atgataatga ttgctaatcc tagcgatagg tttacccgat
     2401 agcaagggat ttctctggct tgcaaatgat aaaaattatc atatgatatt ggttatcatt
     2461 atcaatgaaa gagataaaac catgttgcaa cgtatgctgg gcagtggctg gggagtgttg
     2521 ctgccgggat tgctgattgc aggactgacg tatgcggatt tatcgccgga taagtggcgg
     2581 attgtgattc tgatgggatt actgctgaca ccgctgatgt tgtatcacaa acagttacgg
     2641 cattacgttt tgctaccatc gtgcctggcg cttagtgctg gcatcatgct gatgctaatg
     2701 aatttgaatc agggatgaaa aatcaaggaa gaaacaagaa aggaagtaaa gata
//
LOCUS       DAZGCQ010000254         2741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000254 DAZGCQ010000000
VERSION     DAZGCQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.257"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(831..1829)
                     /locus_tag="ACHK07_005209"
     CDS             complement(831..1829)
                     /locus_tag="ACHK07_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO8105382.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
     gene            2546..>2741
                     /locus_tag="ACHK07_005210"
     CDS             2546..>2741
                     /locus_tag="ACHK07_005210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105383.1"
                     /translation="MSPSTEARRSGNRTANHFAVFVPQPFRAIDKLAVGGESHEVRTY
                     TISTSFRLCAVCDAVKTADNR"
BASE COUNT          686 a          788 c          641 g          626 t
ORIGIN      
        1 aaaaaaaaat ccccctgtgt cagggggccg tcaaagtcaa tttcctgatg atagttgttt
       61 tgaggtgttg ctatgcccgc tcaatttgcc gcaggaacac cgcaaaaaca actccgatga
      121 cgggacagga tgtgtaatcg tataaccgcg cacgcacgcc gatacgatta ccacctggtc
      181 agggctacgc cccgacaccc tcgacaacct gcccgggaca cgttaaaacg acatctgcgc
      241 gtttctgaag ctcccattaa ccatggggat ggtattagcg ccgggatgcg acagaaacgc
      301 gcttaaaacg cccctgaacg catcaaacgc gaaaaaacga acaaaacctt gcctgcctgc
      361 aaaaatcacg ttagaacgcg tctgagcgcg tctgaggggt taaaattcac tgtgcgtcaa
      421 atggggattg ttacaccccg ccctcaaatg cgctcacttc cccttcctgt ccatgtgcgg
      481 aaatgttgtt gctttgtatc ttttgtgtta agcaacggat gagcgtgttt ctttcgcatt
      541 tccactatca tcaacccacg aagacctgta gaaacacgct ccccccctcc gactactgga
      601 cagcagaatc acaccacgct aaacatgaca tgcgtgtttc tggtgagcga aaagctgtca
      661 gccgtggact gccgtagcag agacccggaa acgcgcttaa aacgcttctg agtgcgtcaa
      721 aacaaaaaac gagtaaatcc ttgctcgttt ctgaaaatca cgttagaacg cttctgagcg
      781 cgtttctgaa tattctccca gtgcatgaat agttcgcgcc tgaaacgcgc tcaatcctcg
      841 ccacttagct gacgtttatc tttcgcccga tcgcgctggt attttttgac aaccggcacc
      901 cacgaaaacg ccagacggga accgtcatct gtaccctctg ccatgtcatc cgccatgatt
      961 aaatcctcgt tcgtttcccg ctccagttgc agaacgttct taagcgctcc ccccgtagca
     1021 acaaaacgac gcttgtgtac ctgccgtgtt aattcgagga accattcagg atcagccagc
     1081 atatcagcag gctttgtgga atatttcaga gtttcaacca ccgcgccgcg cagctgttcc
     1141 gcaacaacac aaggtaaaga ttcatcgtct tttggtttcc gtgatttcac cactctgata
     1201 tccacattcg ggtcatacgc cacccgcaga caatcacgcc acagttccac ccaacgggca
     1261 tgtttcacat actcacggcc ggacaacatc gaagggggaa ccatcagcag agtgtgaaaa
     1321 tggggatgtg cagtaccatc cttgccgcgc gttacttccg tggtacgaac ccaacccaac
     1381 acatggcgaa attccttacg ctctttcaac cgcttaaacc ccgcattcat cgcggtcagc
     1441 gtctcaccaa gcgcgtcaat ggaacagttg cgcactgtca gtgtgagaaa caaccagcgc
     1501 gcgccgggat gttccgtcac aatctttggc agggactgat agaaacgggc ttgccacatc
     1561 atggaacgcc tccactgaca gatcggacag tgccggacgc gacagaaccg tgcatcacgc
     1621 aaccgcaggc gcgtttctcc ggtctctgtg tttgttgacc acccaaaccg caaaatacca
     1681 ctacaagccc cgacacgttc gccgtagtgc tcaaatccgg cagcacgcag ataaataccg
     1741 cccacatcgt cggaaaagct tcggtgttca tcccacggtt tatctgctgg agagtaatcc
     1801 gtcagattct taagttcagg atttttcatg tctgccgtcc ccttgcgagc gacagacgaa
     1861 attgcgtgtt atcattcctg tatcacctgt accaattgtc gtcaaatcag ttcatgttct
     1921 aggtggtcgc gcccctggag tggtggaaga ctcgcagggg ctaatttata accactcccg
     1981 acaaaatact ctcaaaccta tttacacgca aatccgcgct caggccagat atggcaaggg
     2041 atagagctga ttcaaaaaat cgcatttatc ccttaagtat aaatacaagg aacgcgcacg
     2101 aaagcagcag caaaatcgcc tgttttttgt tcgatttttt gagggcaaaa acaacaacca
     2161 cgaaagcgtt ttcccgacaa aacacccaca ggcttttcca ccaattttgt gaacaacaaa
     2221 aaccgtcttg caggtggttg gcatcactcc gtgacaccaa ccacctcctc cagtgttttt
     2281 tccgtgccat cgtcaatcac aaaatcggaa cccacgccgg aggcgcggga ccccacgatt
     2341 ttctgtttga cgaattcggg aacggtgagc agtacgcgga attttttcac gtgccgcgaa
     2401 gcgttggcag ggtgtcgggg cgtagccctg accaggtggg gaatgtctga gtgcgcgtgc
     2461 gcggtccgac attcccacat cctgtcccga tttctgcgag tttgtgttac cggaacgctg
     2521 gcgcaatatc ggcagttttt gcgccgtgtc gccctccacg gaagcgcgca gatctggcaa
     2581 ccggacggca aatcattttg ccgttttcgt gccgcagccg ttccgcgcga tcgacaaact
     2641 ggcagtcggg ggggaatctc acgaagtgcg cacttataca atctcaactt cgtttcgatt
     2701 gtgtgcggtc tgcgacgcgg tgaaaaccgc agataacagg c
//
LOCUS       DAZGCQ010000255         2594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000255 DAZGCQ010000000
VERSION     DAZGCQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.258"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..193)
                     /gene="ynfU"
                     /locus_tag="ACHK07_005211"
     CDS             complement(23..193)
                     /gene="ynfU"
                     /locus_tag="ACHK07_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO8105384.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            412..627
                     /gene="essQ"
                     /locus_tag="ACHK07_005212"
     CDS             412..627
                     /gene="essQ"
                     /locus_tag="ACHK07_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8105385.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            632..967
                     /locus_tag="ACHK07_005213"
                     /pseudo
     CDS             632..967
                     /locus_tag="ACHK07_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(1131..1391)
                     /locus_tag="ACHK07_005214"
     CDS             complement(1131..1391)
                     /locus_tag="ACHK07_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105386.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASAIKLTEEIYPPQKSSQNSCE"
     gene            1505..2038
                     /gene="rrrQ"
                     /locus_tag="ACHK07_005215"
     CDS             1505..2038
                     /gene="rrrQ"
                     /locus_tag="ACHK07_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105387.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEH"
     gene            2035..2532
                     /gene="rzpQ"
                     /locus_tag="ACHK07_005216"
     CDS             2035..2532
                     /gene="rzpQ"
                     /locus_tag="ACHK07_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HFO8105388.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KKYQM"
BASE COUNT          737 a          506 c          651 g          700 t
ORIGIN      
        1 tacttgttac gaataaaatc aatcaagcta cagctttaag atttgtctgg aatactttgt
       61 tgcaatgagg gcagatcaaa agggcacctt tttgtactct tgaaaaactg tgttctgact
      121 cttgggtgca gtttgggcag gaacatttaa cgagataatt acggcgtgat tttgagtctt
      181 tacgttctga cataggcttt tcctgtataa atggccgtat acagtacact aaatatgaaa
      241 acatatctcg tattattatt taatatatga ttttctttta aaataattac ccacattttt
      301 aatgtgtctg ttttttagcg ccgttgagaa caacgtttgc tataaaaact aacccataga
      361 ctccgatctt ttcaaacata ttgcaccatc tgtgtacatc ggggtgagga tatgaaatca
      421 atggataagt taacaacagg tatcgcctat ggcacatcgg ctggtaatgc tggtttctgg
      481 gcattgcagt tactcgataa agtaactccg tcacagtggg ctgcaatcgg tgtgctgggt
      541 agtctggttt ttggcctgct gacgtatctg acaaaccttt atttcaagat taaagaagac
      601 aggcgtaagg ttgcgagaag agagtaatcc aatgacacaa gactatgaac tggttgtgaa
      661 aggagtccgc aattttgaga ataaagtggc ggtaactgta gccttacggg acaaaaaacg
      721 ctttgacggt gaaatttttg acctggacat ctcgctggac cgtgttgaag gtgccgcgct
      781 ggagttttat gaggcagcag ccagaatgag aatcagacag gtattcctgg atgttgctgc
      841 cgggttatgt gtaggggatg agcagtcgcc ggaaaagcgc cccataattt tagaggcgca
      901 gaatgtgtgg ataacctaca aaggaaagct accgggaaga attactggtt ctctgaagac
      961 gccaccgacg gcattgcggt cagaaaaaga tgatattgaa tcgcccattg aaaaactgga
     1021 gggcagcgtc gctgatctga ataaaaaatt gtcggtgctg atcccttctg aagatgaaaa
     1081 aaaacgccgc gatgagcagt ttgcggcgtt ttacgattat tgcattgaag ttactcgcag
     1141 gaattttgtg aagatttttg aggagggtaa atctcttcag taagcttaat ggcggacgct
     1201 gcaattaatt caggaaggtc cgcaaggtca tctgtcagtg gatatgatga aaaatcggcg
     1261 gcagttctgt taagaagcgc tttaactaat tccttttcct tctccggcaa caagttgatt
     1321 agagctacga ctgcttgcct gagtgcgatt aaatcagcaa aagtttgttt tggtagattt
     1381 gtgtaatcca tagtcacctc tgtgtttatc agattgacat cctcctcccg ccagtgccca
     1441 tcactggtga ggtaagattt aacatatccg gggatttgaa gccgataaat cctgataaat
     1501 atccatgaac gtaaaaatca gatacagcct gtcggctgct gttctggcac tgattgccgc
     1561 cagtgctcct gcccctgaca ttctcgatca gtttctggat gaaaaagagg ggaatcacac
     1621 cactgcatac cgcgatggct ccggcatatg gaccatctgt cggggagcaa cgatggtgga
     1681 cggtaaacct gttattccgg gaatgaaact gtcgaaggaa aaatgcgccc aggttaacgc
     1741 cattgagcgg gataaggcgc tggcatgggt ggagcgcaat ataaaagttc cactgaccga
     1801 gccacagaaa gccggtatcg cgtcattctg tccctataac attggccctg gtaagtgttt
     1861 tccgtcgacg ttttataagc ggcttaatgc cggtgatcgt aaaggcgcat gcgaggcgat
     1921 tcgctggtgg ataaaggacg gtggacgtga ttgccgcatt cgttcaaata attgttacgg
     1981 tcaggttatt cgtcgtgacc aggagagtgc attaacctgc tgggggatag aacattgaat
     2041 cagatattca cggtgatttt gctcgtgttg gtaggatttg tcgtaggtaa tgtctggagc
     2101 gacagaggat ggcaaaaaaa atgggcggag cgtgatgctg ccgaattatc tcaagaggta
     2161 aatgtccaat ttgctgctcg aataattgaa caggggcgaa ctatatcccg tgatgaggct
     2221 gttaaagatg cacaacagaa agccgctgaa atttctgcca gggctgctga tctgtctgat
     2281 agtgttaacc agctgcgtgc cgaagcaaca aaatatgcca tacgccttga cgcagcgcag
     2341 cataccgcaa atcttgccgc tgccgtcaga ggcaaaacaa ccaaagccgc cgaaggaatg
     2401 ctcaccaaca tgctcggaga tattgcagca gaagctcagc tttatgctga aattgctgac
     2461 aaacgctaca tcgcaggagt gacttgtcaa cggatttatg aatctttaag agataaaaaa
     2521 tatcagatgt agattaatat taaatcggat tatttttagc gctgaatgtg aaatttaaat
     2581 aaaaaggact cttc
//
LOCUS       DAZGCQ010000256         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000256 DAZGCQ010000000
VERSION     DAZGCQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.259"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..189
                     /locus_tag="ACHK07_005217"
     CDS             76..189
                     /locus_tag="ACHK07_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO8105389.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            258..491
                     /gene="ydfK"
                     /locus_tag="ACHK07_005218"
     CDS             258..491
                     /gene="ydfK"
                     /locus_tag="ACHK07_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO8105390.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKV"
     gene            872..1462
                     /locus_tag="ACHK07_005219"
     CDS             872..1462
                     /locus_tag="ACHK07_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8105391.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(1560..2135)
                     /locus_tag="ACHK07_005220"
     CDS             complement(1560..2135)
                     /locus_tag="ACHK07_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8105392.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(2135..>2546)
                     /locus_tag="ACHK07_005221"
     CDS             complement(2135..>2546)
                     /locus_tag="ACHK07_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105393.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          766 a          578 c          502 g          700 t
ORIGIN      
        1 ctacgaactt taagaacacc caggataaaa attgtcaact atattatata taacaccata
       61 ctaattcgag gctatatgaa cagcatactg ataatcactt cactacttat catattcagc
      121 atttttggtc atgctctaat aaaattaggg attggcatat ccaataaccc ggacaaaacc
      181 gatgtataag tcaacatatc ctgaatcaga catacaatat tgcaatgaaa accaataata
      241 ttttaaggaa tatcttcatg aaatcaaaag acaccctaaa gtggttccct gcgcagcttc
      301 ctgaagtcag aattatcctg ggagatgctg tcgtggaagt agcaaaacag ggaagaccta
      361 tcaataccag aacattgctt gattacattg aaggaaacat aaagaaaaag tcatggctgg
      421 ataacaaaga attattacaa acagcgatat cagttcttaa agacaaccaa aatttaaatg
      481 gtaaagtgta ataccccccc ctgaaaaata atttttcaat gcattaaaaa aatatcaaca
      541 actacgttat tccgcaacaa cctacttttt ttaccatttt tccactttaa caacattttg
      601 ctccactttt ccacgaccaa acaacttaaa atctggttga aataacatac aacactatta
      661 ttcgtcttcc tcgagtccgc ccggagttcg aaaaacaaac cgagttaaag ccatttttca
      721 aaaaatcgat ttttggttac accaaaatta cgggactgca tccgaattcg tttattttcg
      781 aacgtataca tacaaatatg tacaataata agcgaaataa tttttggata tgcattatga
      841 tatgtacacc aatttcgtat agagtctcac tatgtctcga atttttgctt actgtcggat
      901 atcaacgctg gatcagacca ccgaaaatca acgccgggaa atcgaaagtg caggttttaa
      961 aatcaaacct cagcaaataa tcgaagaaca cattagcggc tcagcagcaa ccagtgagcg
     1021 tcctggtttt aaccggttgc ttgctcgtct gaaatgtggt gatcaattga ttgtgacaaa
     1081 actggatcgc cttggttgta atgcaatgga tatcaggaaa acagtggaac aactgaccga
     1141 aacaggtatc agagtgcatt gcttagcatt ggggggcatt gacctgacca gtccaacagg
     1201 gaaaatgatg atgcaagtaa tttcagcagt cgctgaattt gaacgagacc ttttacttga
     1261 acgcactcat tccgggatag taagagcccg gggcgcaggg aaacgttttg gtcgaccacc
     1321 tgtgttaaat gaagaacaga aacaggtggt attcgaacga attaagtcag gtgtaagtat
     1381 aagtgccatt gcccgggaat tcaaaacctc gcggcaaacc attttaagag ccaaagcaaa
     1441 acttcagaca cctgacatat aaaaaataat cttggtgtga gatgctttac gtcttccaag
     1501 cccccttcct tgccgtaaat ggaaagatac atctaattat agaatttata tgttttaccc
     1561 tacggcagtg ctggccattc aatatcctgt gaagttgacg tatcaacacg gttcagcaat
     1621 acccgatact ttttccatgc ttccagcaac gagatttctt cctccgttgc aatttccaga
     1681 tctgcagcat cctgaagcgg cgcaatatgc tcactggcta cctgcatcag gctgtttttt
     1741 gtttcttccg cctctcggat ccggaacagt ttttctgctt ctgcatcttt cacccaggct
     1801 gtgccgttcc acttctgaaa ctccccttcc ggcgataacc aggtaacatt ttccggtaac
     1861 ggaccgagtt cagaaataaa taacgcgtcc cctgacgcta cgtcataaac cgttttaccc
     1921 cgatggtctt caacgagatg ccacgatgcc tcatcactgt tgaaaacagc cacaaagcca
     1981 gcaggaatat ctggtggtgc aatatcggta ctgtttgctg gcagacctgt atgaggcgga
     2041 atatatgcgt caccttcacc aataaattca ttagttccgg ccagcagatt ataaattttt
     2101 atggtccgtg gttgttcact cattctgaat gccattatgc aagcctcaca atgtagttaa
     2161 atgcgatgtt tttgacggtg ttttccgcgt taccagcagc gttaacggtg atagtgtgtc
     2221 catgtgagcc aatcgcaaca gagtgcgtat gagcaccaat accgacagta tgtgcgtgag
     2281 cacctgcaga tgcagcggtg ccgctgacac tatgagtgtg tgctcctgca gcacttgtat
     2341 tcacactggc cgcagacact accttatgta ctgacccatt ttgtgaccac tggctgactg
     2401 ctgatgcgcc tgtgacacta tgagtatggt ttccggcact tgcggctgta ccgctcacac
     2461 tgtgcgtatg cgccccggtg ttattcgtgg atttagtgcc gtaatcaaac gacgatgtgg
     2521 ttttcgtccc caaatccgta ctggat
//
LOCUS       DAZGCQ010000257         2470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000257 DAZGCQ010000000
VERSION     DAZGCQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.260"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /locus_tag="ACHK07_005222"
     CDS             complement(<1..216)
                     /locus_tag="ACHK07_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO8105394.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGV"
     gene            complement(369..821)
                     /locus_tag="ACHK07_005223"
     CDS             complement(369..821)
                     /locus_tag="ACHK07_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO8105395.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            complement(847..2397)
                     /locus_tag="ACHK07_005224"
     CDS             complement(847..2397)
                     /locus_tag="ACHK07_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO8105396.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
BASE COUNT          708 a          590 c          456 g          716 t
ORIGIN      
        1 tacacctgaa tatatattga cagctcttgg ccaggttgtg acctcaccac tgacattaac
       61 acggcggcta taggtaccaa caataatgaa catcagaaat gccgtaataa acactatgct
      121 tcccagcatg attaaccata gtggtattcc gggaagtaat attgcccgtc cctgccactt
      181 catttttctg ttttctaaag catcctgacg aaacaaaata gctcctctta ctcacacttt
      241 tcctgtaata ccttcactgg ttaagcgaaa tgctataatc aaagccagaa aatacagagt
      301 cctgtcaaaa tagtatcagc gatgcattta ccccgtaatt tacaattatc tctgtatctt
      361 cagaggtact acgttttatg cagccgctca gggatgtatg tcacaatttt ctttcgcctg
      421 cttaaccatc gtacctctcc ttctccccat ggctgaagct gtatgagata gtcaataact
      481 tttcgaccaa agcctggttt acaacagaaa tccagaatcc aaatccttcc atcttcattc
      541 cattcagacg gatgcaacct gaattctgga tccctgagca ggcgcgcttc tgtatcagcc
      601 tcaagatatg cccaggtaat atacgccagt ggaaaacctc gtgaatcaaa caggaattta
      661 atctgattat gctgtatcgc atattcagtc cagtattgaa tggttaacac cggatacaga
      721 ctgtaattac gattgtagag catacaactg accgcaaaac cgatctgttc ataaacagtc
      781 cattcatctt ttcctctgat ggtttccctt atataagaca tgattactat ttcccttata
      841 tattttttac actgtgtttc ttatcggttc aagatatcca aatgcatctc tggaaaactt
      901 aagcataaaa tttttcatat cagaaattgc ttcttgtaaa aaaaggggat catcgccatg
      961 attatccagc aggatggcaa ttttttcgca catattttct ttcctcagct cttccctggg
     1021 ttgcagcctt ttcatggtat gatgttcaat ttctttcagg agtaatgagg catttatctg
     1081 acgtctggta ctgccttgcg tcagccagtg gatggcctgg gaaaaacact gttgtgcaac
     1141 ggtgaaatga acatcattct tgatgctact attcagtgcc tgcttttgat tatgcctatg
     1201 atggattagc gtcacaacca acttttgcaa tggtgaacca tcctgattga caatatcttg
     1261 ggcatgtgat ttctcgcagt attgattgag taatgcaaca aaactgttaa ccagttcttt
     1321 caatatatca ggtatctctt tttgtgttat aaattctcca ggaataccca acagttctag
     1381 ataacagtaa taacatgctt catgaaaagt tttctgaaat atctcctcaa ccttcaaacg
     1441 ggctgcaaga gtgaagtcag ggtcaaacat atattttccg atatacaagc agagatcaga
     1501 cagcccttgc ataagtacca tcggttcttt tgccagttca gctaatctta gtttataccc
     1561 aaaatcagct ggggaatctc ccggaaacag acaactgagt ccgttaaaat ccattaatct
     1621 gccatccatt gcaatattag aaggagaagt tgtcaatctc aggaaacgtg ttcgacaaaa
     1681 tgccatttgc caggcctcac gacgtgccag ttcacataac ccctcaatgc aataaatccg
     1741 gggatcagtt cgtgcttcag catcaatttc ttccggtggt acaggtagat atcctggcag
     1801 cttgtggatc acagatctaa cccgacaggc atcgtgaatt aacagactgg aatactccgg
     1861 ttttacttgg aagtaaggtg cccgttcaaa atgcgccgga cgaacaacag gctcacgtac
     1921 caacagggct cttcgtgatt tcctaccgga gcgctcaaat gccttttcag tatagagatc
     1981 tgtaagtaaa accgcccgaa cccgcacagc actataaggt aatatttgag ccagtacttc
     2041 tccccacaaa gcctcatata ttgcatgaac agcgccgagt gcaccattag aatgacgttc
     2101 gtcagtacct tcgccaacca acggattact tcctattcct ttaacctggt agttaccatc
     2161 aaacccacaa cgtgcaccac ctccattacg ctgaatacca ctgccaccat agcgctctgc
     2221 atggaacact ttggcagaag ctgtactgcc aggatcgata atataactgc aggcattttg
     2281 cagctcgtct ggatttctga catcctctgc acaccagatt aatgaggcct tgatctgcct
     2341 ggcagtgaaa ggcaccaggt tttcattcag ttcagaaagt gaacactgat gactcatctg
     2401 gcagtattct ccgtttcaat tttcataaaa gagtgttcat aaaagagcaa gtaagtgtcc
     2461 tgacagcttc
//
LOCUS       DAZGCQ010000258         2270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000258 DAZGCQ010000000
VERSION     DAZGCQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.261"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..450
                     /locus_tag="ACHK07_005225"
     CDS             <1..450
                     /locus_tag="ACHK07_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105397.1"
                     /translation="APWTRGNNCRHDEKTNNETDKTPPPEGRKEQKLIVPEGDPGEWL
                     RSLTPVQRKQLTQQLKNDQKSTKTANSGGFPAPEGVVYAGAALAKPDMTTRLIASTLS
                     TTGEELHSSAALSVYRGARVRLPDGRIVHWDESTGSLKEVAQLKGDE"
     gene            447..848
                     /locus_tag="ACHK07_005226"
     CDS             447..848
                     /locus_tag="ACHK07_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105398.1"
                     /translation="MTRFNCTLQQREFSVVMTKRLPALTLRGLIRRPQPGDQLEITIA
                     RPDRSTESMTADILNARPWGDSGDFIAALRLRNTGAPEPLTPAGRIMKAYHEKDIRKH
                     NEDLARAMKRINRKAAIASYVHSAARKQHRK"
     gene            868..1062
                     /locus_tag="ACHK07_005227"
     CDS             868..1062
                     /locus_tag="ACHK07_005227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105399.1"
                     /translation="MSTRQQHTINSTRAISDRDLNSMIESAQQLLSRQNPVVEETWWE
                     NLLAALTELRERRNNEGATK"
     gene            1059..1406
                     /locus_tag="ACHK07_005228"
     CDS             1059..1406
                     /locus_tag="ACHK07_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8105400.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEEERLELAQRAEWQQEKSVIEVCKRSEVMKRILFI
                     YGAGGDLPVISVQ"
     gene            1430..1747
                     /locus_tag="ACHK07_005229"
     CDS             1430..1747
                     /locus_tag="ACHK07_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8105401.1"
                     /translation="MTTQIATRGRLAEDPTTRKSSRGTDVTAARLAISLPGHGAEQST
                     TMWLYLIAFGKQHEELAKHKKGDLVQVHGNLQVNRYQDKYGQMKEGWQLLAQSIISSR
                     TAI"
     gene            2002..>2270
                     /locus_tag="ACHK07_005230"
     CDS             2002..>2270
                     /locus_tag="ACHK07_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105402.1"
                     /translation="MKNNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPSQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSS"
BASE COUNT          749 a          556 c          590 g          375 t
ORIGIN      
        1 gcaccttgga ctcgtggcaa taactgtcgc catgatgaaa aaacaaacaa cgaaacggac
       61 aaaacaccac caccggaagg gaggaaagaa caaaaactaa tcgtaccgga aggtgatccg
      121 ggtgagtggt taaggtcact gactccagta cagagaaaac agctcaccca acagctgaaa
      181 aatgaccaga aatccacaaa aaccgcaaac agcggcgggt tccccgcacc tgaaggcgtg
      241 gtatatgccg gtgcggcact cgcaaaaccg gatatgacca ccaggttgat cgccagtact
      301 ctgagtacaa cgggcgagga gttacacagt agtgcggcgc tttccgtgta tcgcggggcg
      361 cgcgttcgcc tgcctgacgg gcggattgtt cactgggatg aaagcaccgg aagcctgaag
      421 gaagtagcac agctgaaggg ggatgaatga cgcgattcaa ctgcacgctt cagcaacgag
      481 agttcagcgt cgtcatgacc aagagattac cggcgctgac gctgagagga ctgataagaa
      541 gaccgcagcc aggtgaccag ctggaaatca ccatagcccg tccagacagg agtaccgaaa
      601 gtatgactgc cgacatcctg aacgccagac catggggaga cagcggcgac tttatcgcag
      661 ccctgagact gaggaacacg ggagcaccag aaccactgac gccagccggg aggatcatga
      721 aggcatatca cgaaaaggat atcaggaaac acaacgaaga tttagccagg gcgatgaagc
      781 gcattaaccg caaggcggcc atagcaagtt atgtacattc agccgccaga aagcagcacc
      841 gcaagtaaca ggagaaccac agacatcatg agcaccagac agcaacacac cattaacagc
      901 accagggcga tcagcgacag ggacctgaac agcatgattg agtcggcgca acagctgctt
      961 tcacgccaaa atccggtcgt ggaagaaaca tggtgggaaa atttactggc agcactgacc
     1021 gaactccggg agcgccggaa caacgaagga gccaccaaat gaagacccca ggaatgaccg
     1081 gaaaggaaat cattttacgc tacctggaaa cgcacgcacg cttcagcgca ccggaactgg
     1141 tagaaaaact gggcgtgaaa agaaccgtca tcaatcaggc cgccggaaaa atggtacggg
     1201 aaggcctgct ggttgtggac gggtacacgg acaagataac gcattaccgg aagccaacgg
     1261 aagaggaacg actggagctg gcgcaacggg ctgaatggca acaggaaaaa tcagtcatcg
     1321 aagtctgcaa gcgcagcgaa gtcatgaagc gaattctgtt tatctacggc gctggcggag
     1381 atctgccagt catatcagtc caataactaa accaccaacg gagacaaata tgacaacgca
     1441 gattgcaaca cggggacgac tggcggaaga cccgacgaca agaaaatcat ccagaggcac
     1501 cgatgtaaca gctgcacgac tggcgatatc actgcccggc catggcgcag aacagtcaac
     1561 cacaatgtgg ctttacctta tcgcattcgg gaagcagcac gaagaactgg caaaacacaa
     1621 aaaaggcgac ctggtacagg tgcacgggaa cttgcaggta aacagatatc aggacaaata
     1681 cggacagatg aaagaaggct ggcagttgct ggcacaaagc attatcagct caagaaccgc
     1741 aatatgatca atatgtgagt aatgatgtga gacgccgggc tggcacaaac aaaaaggcgg
     1801 agaaacatac tccgcctgac acagttttac gtattcagaa gccgtcaaat tcgtcagaca
     1861 cttttttcat attaaccact acgaattcaa atatgtacat aagttaacgt cgataaccac
     1921 aagaacagca caataaccca ccttgataaa gaaaaaaacg atgttatgat ttggtcataa
     1981 gtaaggtgat acgaatcagt tatgaaaaac aatgatcatg ggctgaacac ccttctggat
     2041 atgcatggat accagcacca catggataat ggttactggt ggaagattga agcatacagg
     2101 gttagcccaa gccagttcag accacatgga atacgctaca acctgacatt gcacgatcct
     2161 tataactccc gggtttttgg aatggataac gctcacggaa taaagccacc aaagaaaggg
     2221 aaatttaccg ggcgcattac cgtttatgac catattcaca gaacatcgtc
//
LOCUS       DAZGCQ010000259         2248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000259 DAZGCQ010000000
VERSION     DAZGCQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.262"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(200..418)
                     /gene="ymgF"
                     /locus_tag="ACHK07_005231"
     CDS             complement(200..418)
                     /gene="ymgF"
                     /locus_tag="ACHK07_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO8105403.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(550..2073)
                     /locus_tag="ACHK07_005232"
     CDS             complement(550..2073)
                     /locus_tag="ACHK07_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8105404.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSTTPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          682 a          493 c          470 g          603 t
ORIGIN      
        1 cgattttgtt taacaaataa ctcttcgaca tcacaatcat tgatagttgc catagaaatt
       61 atttcaatga tatataaaaa caactgtgat aacaccaggg ttgacaacta tttgcgcaat
      121 aattcctttc cttcaatgat ccaggcgttc actgtcagca ccccattcag ccgcatactg
      181 acagtgagaa actgaaagat tattgctgat ccctgtggct gacgagtctt tcccttaatc
      241 gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc gtcacaagca
      301 cttctcccag cccggtactg ggttttgcaa taaggatgac caggagagtg atcagcataa
      361 atgcaatgga aaagatgata tagagagtga aataatccag attattacta ttgttcatgc
      421 tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat
      481 agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta tggccttact
      541 gatcgatttt cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag
      601 gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga tattattttg
      661 gttcagatag tcaagttgtt ctttcgtttc gacaccttca gcgacgatac tgatcgaaag
      721 tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt ctgcgttgac
      781 gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat gcagataaga
      841 aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag
      901 aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct cggtgacttc
      961 cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac actcgtcgac
     1021 aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa aaccttccgg
     1081 cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc tttgtgtaag
     1141 cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg aaatatatcc
     1201 accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc cttcccgacc
     1261 atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc gtcgcaggat
     1321 ctcttcaggc gttgtgcttt tattgaaata tctatcaagc aggaaggcag cggcgcaggc
     1381 gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt agaagctaaa
     1441 gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa ccttctcggt
     1501 atagggataa tgtccattaa gcgtcatcac atcacccgtt ggtccaataa tgtcatccgc
     1561 cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc gaatatgttg
     1621 atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga gcaggatagg
     1681 taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg ggacaacagg
     1741 aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta ttccctgttt
     1801 gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa gctgatattg
     1861 ctcctcaagg tcgcattcct taccggcaat gttaaccgct gtttgagtcg cctgtgacgc
     1921 ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca ggtactctgc
     1981 ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa acaagcatat
     2041 cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa agggtttaat
     2101 acatgaaaat gccattcatt cgctcgcgct ggcatctaca gagagcatgg tagagagcat
     2161 aagaaatatc cacgccgaag ataaaatttt ctccatcttg atctaaccca ttaattattg
     2221 agcataatgt aggcagtata aaataaat
//
LOCUS       DAZGCQ010000260         2247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000260 DAZGCQ010000000
VERSION     DAZGCQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.263"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..892)
                     /locus_tag="ACHK07_005233"
     CDS             complement(<1..892)
                     /locus_tag="ACHK07_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105405.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTG
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTEPDPERVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRA"
     gene            complement(889..1305)
                     /locus_tag="ACHK07_005234"
     CDS             complement(889..1305)
                     /locus_tag="ACHK07_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105406.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVDPRLASCLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AMKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(1374..1601)
                     /locus_tag="ACHK07_005235"
     CDS             complement(1374..1601)
                     /locus_tag="ACHK07_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO8105407.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNVLHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            complement(1532..1915)
                     /locus_tag="ACHK07_005236"
                     /pseudo
     CDS             complement(1532..1915)
                     /locus_tag="ACHK07_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1925..>2247
                     /locus_tag="ACHK07_005237"
     CDS             1925..>2247
                     /locus_tag="ACHK07_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105408.1"
                     /translation="MWRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLERI
                     TCVFHRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQAVS"
BASE COUNT          429 a          586 c          687 g          545 t
ORIGIN      
        1 cggcgcgtaa cagttcttca cgccagatgt cccggaggtg tcttaactga ctgttccacc
       61 atgtcgcaca ggaaaggcgc atgaccgcac ccggcacccg ttccgggtcg ggctcggtgc
      121 ggcggtcggg cctgatttcc agcgacggcc agcagggtgg cgtgataccc agttttaacg
      181 ccatgtgtgc cagtggctgg taggtcagta accaggtgag caggtcatcg tcacccctgc
      241 cggtcttttc gatgacctca tcacatgcgc gctccagtgc ggtggaaaac gcggtggcgg
      301 tcagggtggc aaggcgctgg atttcgtcac ggctcagctg cggcagcata agcagttcat
      361 ccagccgttc gcgggcggcg gtggccttaa aggccggggt ggcaaagcgt tcattcacac
      421 gggcgatacg tttcaggcgg gcacgcagcc cgtgacgaac gaagttatcc gccgcatatg
      481 cgctgttatt cttaagccct tcccggcgtg cgctctcctg tttgcggctc aggcgcgcca
      541 ggtcacggat gagcggaccg gcaagaaacg gcggcagggc gcgcagctct gccatggctt
      601 cagataccag tgttggcgga ttttcgtgtt tgtgccgcgc cagtttttcc agcctgatcc
      661 gacgctcttc gtcgtggtaa agatgcgaat taattcgctt cccttcgtct gtggttgtca
      721 ggaaggcgac ggtttcttca tccagtctgg cgtcagtgcg aatatcctcg ttgatgtagc
      781 tctgaatggc ctggcgtaat gcggttggct gcgctctgcc gaacgtttca ggatcgataa
      841 ccggtagcgg ggcgttccac gggtacgcca gttgtgcatt atggtcgctc atgcgtcgca
      901 ctccccggca gtcgccgcct gaaacgccgc gcggtacagc acgagaaggc gctggtattc
      961 accgataaag tcttctgcgg tgtatatgcc tttcatggcc acgctgtgcg ttgtcatttc
     1021 cagaacaagc gtcatctctg acgacgacca gctgatggcg gtgctgtcgg ttccgttgat
     1081 gtgaaataaa ccaaaaccat caccatcacc tttcacgtcc acgcggtaac ggtcgtcaac
     1141 ggtgaaggag aaatccccgt atgtggtgac gcgtctggcg gcccgtttac aggcgcggcg
     1201 gtaatagtcg tgccagtcgt tcaggcagga agccagccgg ggatccactg cccacatcca
     1261 gttaaaaaaa tcctgaatat ctgaaagtcc cttaacgttc ttcatgagaa cctccggtaa
     1321 cagacgtgcg gaagcctccc gcgcacgggt gcgggatggc ttcaggatgt tgtttagtgg
     1381 tggtgttgct ggttgataag gtcctgcagt tctgccaggt cttcggccag atagctgaag
     1441 actgccgcgt aatacccggc agcgataccg tcgttaagtc ggtgtaatac gtttgactgc
     1501 atgatgaagg agacagatgc agcccgatgg agtctgcggt tgatttcagt gcggatgtga
     1561 agacgctcag cgcgggcgcg ctgttgtttg cggtttgcca tggtgtggcc tcgtacgtag
     1621 taagttctga aaactcacca acccgaggtt ccaagctcag ggtggcgaga cgtacagggt
     1681 tggaactacc ggctacgcac gaacccggcc cgaccgaagt cggccccgta cgccccgcca
     1741 taattctgac gtgaaaaaga cgtggcgata cagtacgcac aaaaaaaccg ctggcgcggt
     1801 tgtgcgcgtg catagtcggc ggggttccaa tcccggcacc tgttttatga ggtgcgggaa
     1861 aactgtaacc cgaccaggcg aacgctggca agcggttttt gtgttgcgtg ctgatgtggt
     1921 tagaatgtgg cgaattaata tcacctggca actggtgatt gtctgcatac gcggggtgtt
     1981 tctgatgagt gataagctcc cttctgatta cgacattctt tttgacctgt ggtacagcta
     2041 ctggcttgaa cgtattacat gtgtgtttca ccgacgcgtt tctgctgctc tgcgaatcag
     2101 cgcctttctg gttggtgttg cggcgtttgt cctgtgtgat tttgttgtgt tggtactctt
     2161 cagtgtggtt ctgtttctgc tttggcagtg catggctgcc agttttgaac gcagggctga
     2221 aaaggctgaa tggcaggcag tcagtta
//
LOCUS       DAZGCQ010000261         2239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000261 DAZGCQ010000000
VERSION     DAZGCQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.264"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            407..736
                     /locus_tag="ACHK07_005238"
     CDS             407..736
                     /locus_tag="ACHK07_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105409.1"
                     /translation="MWNICFLANGMPDTWFYTDDASVTKEWGRKYCDNVEVFPLCYMH
                     DGPVFRGRYIDELSAARSRMLHMHHILPERTRYRENIHDAECIREIHEAPLQAPGILQ
                     KYKRTKS"
     gene            746..937
                     /locus_tag="ACHK07_005239"
     CDS             746..937
                     /locus_tag="ACHK07_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105410.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            971..1294
                     /locus_tag="ACHK07_005240"
     CDS             971..1294
                     /locus_tag="ACHK07_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO8105411.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(1682..2104)
                     /gene="subB"
                     /locus_tag="ACHK07_005241"
     CDS             complement(1682..2104)
                     /gene="subB"
                     /locus_tag="ACHK07_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO8105412.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSISACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(2121..>2239)
                     /locus_tag="ACHK07_005242"
     CDS             complement(2121..>2239)
                     /locus_tag="ACHK07_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO8105413.1"
                     /translation="SLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          635 a          571 c          499 g          534 t
ORIGIN      
        1 aatgtcggct ttttacgtcc atatattcca cttatccgga actgtgacac agaacctgaa
       61 tacaggattg atcaccggtg cccacaaatg gcacattttc agtaagattc tgaatgttac
      121 attccccctt catgtgtgac acagctgtca cagagaacga aacggaaagc acatgaaaaa
      181 cataaaaact cacaccggac tccttatcca caaagaacaa acacggcgcg ttcgtcttca
      241 tgaaacaccg gccgcctggt gccacacacg ccgggaatgc tacagcaaaa cgaccggtcg
      301 tcgctgcggc agccctgaca gtctcagcag gctgattctg agcagcattc agcccataga
      361 atgagataac ccgtatgaat gatgacagac gtctacggta cccgatatat ggaacatttg
      421 ctttctggct aacggcatgc ccgacacatg gttttacacc gatgacgcct ccgtcacaaa
      481 agagtggggg cggaaatatt gtgacaatgt ggaagtgttc cctctgtgct atatgcatga
      541 cggtcccgtt ttccgggggc gctatattga tgagctcagc gccgcacgga gcagaatgct
      601 ccatatgcac cacatactgc cggaacgaac ccggtacaga gaaaatatcc atgacgcaga
      661 atgtatccgt gaaattcacg aagcgcccct gcaggctccc ggcattctgc aaaaatacaa
      721 acgcacaaaa tcataaggct gttctatgtg gatactgatt ttctggctct catccccctt
      781 taatgtctcc tccggcgatg cccggcaacc cacactggcc gttcacagtc aggagttccg
      841 tactgaaaaa gcctgtcggg atgccttcag cgcgctgaaa aaagaaaatg tgggagaatt
      901 cgctctccgg ggcgtctgcg tccccggcgg agaataaacc ggagatgatg attctccaca
      961 ggagtattca atggcatacc attccccgga tgtcacactg cattatccgt atctgcgccg
     1021 gatgtcctgg gcgcagataa cggcagcagc tgaagatgcg gaaatacatc atgattatgc
     1081 ccgggcatta atattgtggc agcatgcata tcatgctgcc acgctgactc tcaacaaaaa
     1141 cctggcagtt gctaaaatcg acttctgtat taaacgtatc cggatgcatg aacaaatgag
     1201 caggataatc cggaagactg gcacagacga aatactcttc cgtctgagta aacaccatca
     1261 tttatatgag aaaaagaaaa caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg
     1321 ccatgctcac tgcgggggca tttttgtact ggctgttcta accgggtaac ccgttgatac
     1381 acatagtaca ataatgtttt atgcaggctt acgcgccaga ctgccatcag tagtgcattg
     1441 cgtgccagct cgcggacagg ggcagtgacc agccaccaca tgagtaagca tcatttcagc
     1501 gccatcaggc agctcctgaa actcctgcga gttgaaaaag catcctcctg cattagcaat
     1561 actggcttgt cagtgccagt gtgatataag acgtaatgtc aaagttcgtg gtgttgacat
     1621 atttctgtcg caaccaaagt atgctcccgc ccagtatata tctgggcagg agcatcattt
     1681 attagttctt tttcctgtcg gggccaaaac attctgtcga tgtggcgcag gttgataacc
     1741 caacaagggc attggacgtt aatgccttca caaagggcgg atacgtccat accccgggtt
     1801 cataataaat cctgaccaac tgacctgttg tgtaaaaata tagtgcctga ttatataatg
     1861 tggaaaacga aggcccccag acagatgaat tcttcatcga gcaggcactt atggaagaac
     1921 caccaggttg tttcccttca atacaaaaat aagggttatt atctatttgc cctgtatgaa
     1981 actgatcaat aacaacgcct gaaaacatgc catcccgggc atcgccagtc cactcagcca
     2041 tggcaggatt aagggataaa catcccataa cacctgcaca tacaaaaaac ctcttaatcg
     2101 tcatattcac ctccggaatt ttacagttct tcactcatcc ttccctgacg gacaggaata
     2161 taatttttct tgcagaacat gctaattgct ttttctgcat taagaactct cccttccgtc
     2221 accttatctg caagtgagg
//
LOCUS       DAZGCQ010000262         2230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000262 DAZGCQ010000000
VERSION     DAZGCQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.265"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            244..807
                     /locus_tag="ACHK07_005243"
     CDS             244..807
                     /locus_tag="ACHK07_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8105414.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            804..>2230
                     /locus_tag="ACHK07_005244"
     CDS             804..>2230
                     /locus_tag="ACHK07_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8105415.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLLYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGA"
BASE COUNT          501 a          535 c          717 g          477 t
ORIGIN      
        1 acaccgcgcc ccctctcacg cagagaaaaa attcccgttt cagggcagtt aacatgttaa
       61 ctggctgtcc gggcattttt gcggttttta tctttattat tcagtttgtt gcgtggaaaa
      121 aatgttaaca ggctttttca gcaaatgtta accaggcagc agttaacatt tgcggcatga
      181 gacgccggga aaaatgggct gaaccatacc cggctgagtg cgttctggac ccgggaggag
      241 gctgtgctga caacgcaaaa acgaaaattt gcgctggcgc tcatgtccgg gaaaaacaaa
      301 acagcgtcag ccattgccgc cggttattcg gcgaagacag ccagggttaa aggctcgcag
      361 ctggcaaaag atccggaggt gcttgcgttt atagcccgta aacagtgcga gacggtggag
      421 gtggatgagg ttcctgttta ccggcagaaa aaatcagagc aggaggataa accccgtcgc
      481 cgtgaggcgg ctgcaatacc acagccggac gaaacaaatc cggagatgcc accgcccgtg
      541 gtgatatctc ctggtattga gtatatggag gacggtcttc ccgatccggt gaaagcgatg
      601 gggcgtcttc tggtggagaa cattaatacc gaccccaggc tggcgctgga tgcggcttat
      661 aagctggcgc agttcacgca ccacaaaaaa ggggatgccg gtaaaaaatc ggcaaaaggt
      721 gacgcggcga aaaaagcggc taaccgtttt gcggtgccac caccaccccg actggtggtg
      781 aataatgata atgagggcaa cggatgatac ctgtgtggag cacggcctgc ccggactggg
      841 cagagcgcct gaaaaagggg ctgtcgatta ttccggcccc gatttatccg gatcaggctg
      901 cacatgcact ggcgattttt aaacaactgc ggattgtgga tgcaccgggc agcccgacgt
      961 tcggggagtc ctgtgcaccg tgggtgtttg acctggtggc ggccctgttt ggctcctacg
     1021 atgcgcagac cggtgtacgc catatcaagg aagtttttat ccttatcccc aagaaaaaca
     1081 gcaagtccac gctggctgcc gggatcatga tgacggcgct gttactgaac tggcggcagg
     1141 cggcgggcta caccattctg gccccgaccg tggaggtggc ggctaacgcc tttaatcctg
     1201 cgcgggatat ggtacgacgg gacgatgatc tggatgacct ctgtcaggtg cagacacata
     1261 tccggaccat cacccatcgg gtgacggaca ccacccttaa ggtggtggct gccgatccga
     1321 atacggtatc cggtatcaag tccgtgggga cgctgattga tgaactgtgg ttatttggca
     1381 agcagtacaa agcggaggac atgttacgtg aagccatagg tggccttgcc tcccgcccgg
     1441 aagggtttgt ggtgtatacg accacccagt cgaatgaacc gcccgccggg gtgttcagac
     1501 agaaactgct gtacgcccgg gatgtccgtg acggcaaaat tcatgatccg cactttctgc
     1561 cggtgatatt tgaacaccct cctgaaatgg tggaaagcgg ggctcacctg ctgatggaaa
     1621 acctcgccat ggtcaatccg aatctcggtt attcggtgga tgaggctttt ctgtaccggg
     1681 agtaccgtaa agcccgggag gctggtgagg aagcatttcg tggcttcatg tcaaaacatg
     1741 ccaatgtgga aattggtctt gccctgcgtt ctgaccgctg ggcgggtgcg gatttctggg
     1801 agcagcaggg caggcgcgtc agcctggacg atatcctgca gcgcgctgat gtggtgacgg
     1861 tggggattga cggcgggggc ctggatgatc tgctgggaat gtacgtgatt ggccgtgaca
     1921 gggaaacccg cgaatggctg ggctggggcc atgcctgggc gcatgaaacc gcggtggtca
     1981 gacggaagag tgaggcatcc cggtttcagg attttgtggc ctgtggagat atgacgattg
     2041 tccgtcgggt cggggatgac acggcggaag tggcggagta tgtgcgtcgt attcatgagg
     2101 ctgagttact ggagcatatc ggtattgacc cgtcaggtgt ggggcagatt ctggattcac
     2161 tggtggaagc cgggatcccc gacggaattg ttgtggggat aagccagggc tggaagctgg
     2221 gcggggccat
//
LOCUS       DAZGCQ010000263         2210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000263 DAZGCQ010000000
VERSION     DAZGCQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.266"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /locus_tag="ACHK07_005245"
     CDS             <1..481
                     /locus_tag="ACHK07_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8105416.1"
                     /translation="TIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAF
                     DYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYNYSPY
                     DDYSVIYRMGQCQIGAKNIDNAMQCFFHIINNCEDDSVKSKAQAYVELLTDNSEDNN"
     gene            815..2191
                     /gene="ygeH"
                     /locus_tag="ACHK07_005246"
     CDS             815..2191
                     /gene="ygeH"
                     /locus_tag="ACHK07_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO8105417.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSNIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          803 a          369 c          368 g          670 t
ORIGIN      
        1 aacaattgaa atattcaata atagtgatga atgggcaaat caactaaaac acgcattatc
       61 gaaaggagaa aacctggcat tactgcatgg tttaactcct gatatccttg atagaatata
      121 tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa tttattataa
      181 atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaaggttttg catcagtatg
      241 tcagtcaaaa aagaaatatc aacaagcata tgacctttac aagctaagtt ataattattc
      301 gccatatgat gactattcag ttatttatcg tatgggtcaa tgtcaaattg gggctaaaaa
      361 tatcgataac gcaatgcaat gtttctttca tattattaac aattgtgagg atgatagtgt
      421 taagagtaaa gcgcaggcat atgtcgaact cttaaccgat aattcagaag ataataacta
      481 agcattacaa tcatttggtt aaatttgctg gttatagtgc attaaccact ataaccagca
      541 cctcttatct tttcttaaat cagcatctta aaacagttaa gagcatacaa taaatcatta
      601 tttttcaatg ctttagagaa tattttactt tatcaaaatt gaataaggct atttcagatt
      661 acatcactga attcctaaga taaattagtg aacacaacca tagatatgtc gaatgtaatt
      721 tcctgtagcg ttaatgacac ataaatatga atagccataa tttctattgc ttatgcagga
      781 tgcaagaaac caattttttc atagaggtta actaatggac ttagaaaata aattctcata
      841 tcattttctt gagggattaa cgctcacgga agatggaatt cttactcaag gaaatgagca
      901 agtttatatt ccaccgaaag agttaggtgt attaatagta ttacttgaat ccgctggtca
      961 tgttgtactg aaagatatga tcatcgaatc agtatggaaa aatattattg ttagtgacga
     1021 gtccctgaca agatgtatct attctttgcg ctgcattttt gaaaaaattg gctatgatcg
     1081 ctgcatagaa acaatctacc ggaaaggtta tcgtttcagc gggcaggttt tcaaaactaa
     1141 aattaatgaa gataatactt cagactattc catagctata ttccctttca ccacttcatt
     1201 gaatacactg gatccattaa tacttaatca ggaattagta caaatcattt caaataaaaa
     1261 aatcgatggt ctctatacct atccgatggc tgcgacaaat ttttgtaatg accacatatc
     1321 tcaaaattca ttcttgagca gattcaagcc agattatttc gttacaggaa gaataaacca
     1381 gaataatgca gtgaacactt tatacattga gttgatcgac gctaaaaacc ttttcctcat
     1441 cgccagtaat catctccctg ttgatgaact acataataca tcacaattta ttatagataa
     1501 tatccttcaa acggtacata aaccagaacg atctgtaaga ttagctaagc aggaccaagg
     1561 atataagaat cattatttat cagatgaaat gttagccgga aagaaagaac tttacgagtt
     1621 cacccctgaa agcatttaca gggccatgac tatatttgat ggattacaaa ataaaagtga
     1681 tatacagacg ctaaaaacag aatgttattg ccttctagcg gaatgccata tgtctttggc
     1741 acttcatgga aaaagtgaac ttgaacttgc tgctcaaaaa gcattagagc ttttagatta
     1801 tgtatcaaac ataaccaatg tcgatggaaa aattttagct attatgggac tgataactgg
     1861 cctgtctgga caagcaaaag tatctcatat cttatttgaa caggctaaga tacactcaac
     1921 tgatatagcc tctctctact actatagggc acttgtcaac tttcataatg aaaaaattga
     1981 agaggcaagg atttgtatag acaaatcact acaactcgaa cccagaagac gaaaagcagt
     2041 tgtgataaaa gaatgtgtag atatgtatgt gcctaacccg ctcaaaaaca acatgaaact
     2101 ctactataaa gaaactgaga gtgaaagcca tcgagttata attgacaaca ttttgaaact
     2161 aaagcagctg acgagaattt gtatgcgata aatttttatt gacgcaattt
//
LOCUS       DAZGCQ010000264         2196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000264 DAZGCQ010000000
VERSION     DAZGCQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.267"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..107)
                     /locus_tag="ACHK07_005247"
     CDS             complement(<1..107)
                     /locus_tag="ACHK07_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8105418.1"
                     /translation="MSEDIPLPEINQRYRDDHGALVTVTSVEETRVVFMR"
     gene            606..1103
                     /gene="tssB"
                     /locus_tag="ACHK07_005248"
     CDS             606..1103
                     /gene="tssB"
                     /locus_tag="ACHK07_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8105419.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGSEESISLTFRNMNDFT
                     PEQIARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            1124..>2196
                     /gene="tssC"
                     /locus_tag="ACHK07_005249"
     CDS             1124..>2196
                     /gene="tssC"
                     /locus_tag="ACHK07_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8105420.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPET"
BASE COUNT          537 a          476 c          579 g          604 t
ORIGIN      
        1 cgcataaaca ccacccgggt ttcctcaaca ctggtcacgg tgacaagcgc gccatgatcg
       61 tctctgtatc gctgattgat ttctggtaat ggaatatctt ctgacataga cttcagaacg
      121 gtaaccgttc actaaatttt catgtggtat cactgaatca ggagacgacg tttcctgatg
      181 tgcgttgtta attttctgtt tttaaaaaat attttctctc tcttctttat tttccgtgtc
      241 ttttattgct ctcctctttc atgtccttct ctttttctga tatgccctta aatgctgccg
      301 aatcatcact cctcgtgaat gtcattgctg gtaatcattc ggtataacca tctgtatcaa
      361 aagcactaac cggaataatt ttctgcattt ttgtattact cctggatata agttttgata
      421 aatgctgatt ttgcgatatg tatcagaata aagatctggt tattggcatt aaaacgatcg
      481 atatgtctgt catattgaga atttgcagat cttcgtccta taatgggtga aattaaatca
      541 gtgcacaagg ggaggccttc tgtggcgatg ggctccctgg gatgcaggtt tcacaggaga
      601 acgtcatgag cagttcgttt cagaatgaga tcccgaaggc aagggtgaat ctgaaattag
      661 acctacatac aggtggtggg cagaagaaag tggagcttcc gctcaaactg ctggttgccg
      721 gtgatttcag taacggacag gaagagcgcc cgctgtcaga gcgcataaaa atggatgtta
      781 ataaaaataa ctttaatgct gttctggctg atatctcgcc gtcagtacgc ctgaatgtgg
      841 agaacacgct tgccggtgat ggctctgaag aaagcatcag cctgactttc cgcaatatga
      901 acgacttcac gccagagcag atcgcccgac aaattccccg tcttaaagcc atgctggcca
      961 tgcgcagcct tctgcgggat ctgaaggcga atctgctgga taacgccact ttccgtaaag
     1021 aactggaaaa aattctgcgg gatcccgaac tgagtcaggc cttacgtgac gaactgagtg
     1081 cactggtgcc ggaacaggtg tgattaacgg attaagagag agcatgctga tgtctgtaca
     1141 aaaagaaaat aatgtggcgg gaaatgcggt ttctgaaacg cattccggcg acagcgtata
     1201 tgcttccctg tttgaaaaaa ttaatctgaa tccggtatca gccctgagtg cgctggatat
     1261 ctggcaggat ccgcaggcca tgtcagaggc atcagcagat gaacgtctga ccgcagggat
     1321 gcaggtgttt atggaatgcc tggcgaaagc gggagcaccg gtggagaaac tcgataaggc
     1381 gctgattgac catcatattg cagagcttga ttatcagatc agccgtcagc tggatgccgt
     1441 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
     1501 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
     1561 tctgcgccag gattttgagg atgcgccgga aattattcag agtggtcttt atctgcagac
     1621 ttatgtggcg gagtacgata cgccgggtgg tgagcccatt gcagccctga tttctgcctg
     1681 ggagtttgat gcgtctgcgc aggatgtggc gctgctgaaa aatatttccc gggtggctgc
     1741 gtccgcgcat atgccattta ttggttcagt tggcccggct ttcttccgga aggatacgat
     1801 ggaagaggtg gccgccatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
     1861 gtggaacgct ttccgtgaaa cggatgatgc ccgctatatt ggtctggtga tgccacgtgt
     1921 tctgggacgc ctgccgtatg gtcctgatac cgtacctgtc cgcagcttta actatgttga
     1981 agaggtgaaa ggtccggatc atcataagta tctctggaca aatgcgtctt ttgcttttgc
     2041 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
     2101 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatttgggaa ccggtaatca
     2161 ggtcaaaatt ccgtcagagg tgatgattcc ggaaac
//
LOCUS       DAZGCQ010000265         2166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000265 DAZGCQ010000000
VERSION     DAZGCQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.268"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(154..504)
                     /locus_tag="ACHK07_005250"
     CDS             complement(154..504)
                     /locus_tag="ACHK07_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO8105421.1"
                     /translation="MAVIYIAGPMTGYKDDNRLEFCLADLKLRYAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLLGKSKAAQATE"
     gene            complement(495..713)
                     /locus_tag="ACHK07_005251"
     CDS             complement(495..713)
                     /locus_tag="ACHK07_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105422.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDQGMTRQDSRNPEMHHIRLI
                     NGSHFCATNMEEVRVLTATRNAGGKAWP"
     gene            complement(710..946)
                     /locus_tag="ACHK07_005252"
     CDS             complement(710..946)
                     /locus_tag="ACHK07_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105423.1"
                     /translation="MNSERNKALLRGLRHIAHAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            complement(982..1962)
                     /locus_tag="ACHK07_005253"
     CDS             complement(982..1962)
                     /locus_tag="ACHK07_005253"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO8105424.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKVHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFWRAARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLSSRTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGCYRKPYFPE
                     HEIRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLCVYCDPPYLQFSGRDNFTA
                     YGKPFDEHEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSV
                     GARTREPAPEVIATLRQCDACGHYGGGYCPDCGPVTGDATYCVMFTPAST"
     gene            complement(1987..>2166)
                     /locus_tag="ACHK07_005254"
     CDS             complement(1987..>2166)
                     /locus_tag="ACHK07_005254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105425.1"
                     /translation="VLLPPPERGKKSPRIAAFIPQRCAEFFTHKAPQLPGDKNRFSLA
                     ITQQEQREVMGFINA"
BASE COUNT          434 a          558 c          589 g          585 t
ORIGIN      
        1 catgtgatta gtaaggaatg catgattgcg cgcctctcct ttgttgagga aagtgacagt
       61 cccatcatgg agtgcatgat taatcttgcc tggaataaag cctgtgaggc ggttggcagc
      121 tcttcgcgca atcatgtttc gatctccggt cggttactca gtcgcctgtg cggcttttga
      181 tttgccaagc agctgtgcga cagtttcgcc ggccgcccga ttttccggga atgacaccgg
      241 catgttcagc ttgagtgcca ttgcgtactc agcccgtgcg ccagcggact cttcccatcc
      301 atccagtaag taaatggcat cggcacacat gagcatcgga atacaaatgc tcatgtactg
      361 ctgctggctt aatccgtcag gcagtacggc aggattaaga acaatgtttc cggcgtacct
      421 aagcttcagg tccgccaggc aaaactccag tctgttgtcg tctttgtatc cggtcatggg
      481 accggcgata taaatcacgg ccatgcttta cctcccgcgt tgcgtgttgc tgtcagcacg
      541 cgtacttctt ccatgttcgt ggcgcagaag tgagaaccgt tgataaggcg gatgtgatgc
      601 atctcaggat tgcgtgaatc ctggcgtgtc atgccctggt caatcaggct ttcgtcgact
      661 tcgtactggc ggccgttggc tgtgaaccgg atgacggttg actcctggat cattgctgac
      721 ccctttgtcc ggcagatttc tcgttgagct gctttttaag ggcggcgatt tcactttcct
      781 gatcctgaat cacccggatt gcttccatgg tgatccccag tgagatcgag gaagatataa
      841 cgacctttgg ttttccaacg gcagccgcaa ctttggctgt ttcctcgtgt gcatgggcga
      901 tgtgacgtaa cccccgcaaa agtgctttgt ttctttcgct attcatttcc tgactcctgt
      961 gttactgatt catctgttta ttcacgtact ggcaggggta aacatcacgc agtaagttgc
     1021 atctcccgtt accggtccgc agtccgggca gtagccaccg ccatagtgac cgcaggcgtc
     1081 gcactgtctc agggtggcga taacttccgg tgctggttcg cgggttcttg cgccaacgct
     1141 gcgtggtgcc tgtatgcagt tcatccggta accctgataa atgcgtctgg tttccggtgt
     1201 gtcggaattg gagatggtga tgctggtcat gccgcccgtt tcctgcgaaa ggcggtcaag
     1261 tgctgccctc aggcgaacgt gctcgtgttc atcgaatggt tttccgtagg cggtgaaatt
     1321 gtcccggcca ctgaattgca ggtaaggggg atcgcagtac acgcaaagcg tgttgcccat
     1381 gccgaacagg tgactggcag agttcagcgt atccttaaaa tctgcgtgga taagcagcgt
     1441 tcgggtgctg tttgccttgt ctgcaaatgc gcggatttca tgctcaggga aatagggctt
     1501 gcggtagcag ccgaaaggca cgttaaaatc gccttttccg ttcacccggt acaggccatt
     1561 aaaacagtgc cggttgaggt acaaaaacat ggcggccagt cgcagttcac ggcggtcgct
     1621 tattcccgtt ctgctactca ggaaggcatg attctctgcg ttaaaggttt cgcgaatgat
     1681 gagatagtca cccctgttgt tgtggttcag gaacagggtt ttcgcctcgt cgatcagact
     1741 gtctgtgttg tcccgtgctg cccgccagaa attaatcagc gcaccattgg aatcacccag
     1801 gatgtagcgt ttgtaactgg tgttcatgaa taccgatgcg ccacccacga acggttcaac
     1861 caggcagtca gcttctggca gatgcacctt cagtgtgttc atgatgcggc ttttgccacc
     1921 cacccatttc agcggtgtgc cggataacag ttctttgctc ataacgcggc ctctttgtta
     1981 taggtttcat gcgttaataa atcccatgac ttcccgttgt tcttgctgag taatcgccag
     2041 cgaaaaccga ttcttatcac caggtagttg tggggcttta tgcgtgaaaa attccgcaca
     2101 ccgttgcgga ataaacgcag cgatgcgcgg gcttttcttt ccacgttcag gggggggcag
     2161 caacac
//
LOCUS       DAZGCQ010000266         2161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000266 DAZGCQ010000000
VERSION     DAZGCQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.269"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..>2161
                     /locus_tag="ACHK07_005255"
     CDS             11..>2161
                     /locus_tag="ACHK07_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8105426.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSSASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAADSKTAAASSASAASTSAGQASASATAAGKSSESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKTANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGA"
BASE COUNT          605 a          590 c          621 g          345 t
ORIGIN      
        1 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
       61 acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac
      121 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca
      181 gtacagcgtt attctgttgg tggaagggtt cccgccgtca catgccggga ccatcaccgt
      241 gtatgaagat tctcaacccg gtacgctgaa tgattttctt ggtgccatga ctgaggatga
      301 tgtccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc
      361 gtccgcggtg gcacagaaca cggcagccgc gaagaagtca gccagcgatg ccagcacatc
      421 agcccgtgag gcggcaaccc atgcgactga tgctgcggac tcagcacgcg cagccagcac
      481 gtcagccgga caggccgcgt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc
      541 aacaaaggct actgaagcat caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc
      601 ggctaccagt gccggtgcgg cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc
      661 agccgccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcct cctcagccag
      721 ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgagtgcag cctcgagcgc
      781 cagtagttcc gcttcctcgg caacggcggc aggcaattcc gcgaaagcgg caaaaacgtc
      841 cgagacgaac gctaagtctt ctgaaacggc agcggaacag agtgcctccg cagcagcaga
      901 ctcaaaaaca gcggctgcat catctgccag cgccgcgtca acaagtgccg ggcaggcctc
      961 agccagtgcc accgctgccg gaaagtcgtc agaaagtgcc gcatcgtctg cttcaacagc
     1021 cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgagtt ctgcttccgc
     1081 agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac
     1141 ggctgccgca tcgtcagcca gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa
     1201 agatgaggcg accagacaag cgtcagcagc gaagagcagc gccacgacgg catccacgaa
     1261 ggcgacagag gcagctggta gtgcgacggc agcagctcag agcaaaagta cggcggaatc
     1321 tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     1381 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag
     1441 cacttccgag tcactggcgg caacgccaaa agcagttaag gccgcgtatg acctggctaa
     1501 cgggaaatac acagcacagg atgcaacgac agcacagaaa gggatagtcc aactaagcag
     1561 cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa aaacagccaa
     1621 tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga
     1681 cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac
     1741 aggaaattgg acgactgcac agtttatcga gtggctggat tctcagggag catttaacca
     1801 tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac
     1861 tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc
     1921 gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa ccaacgccca
     1981 gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc
     2041 cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc
     2101 atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata cgcagggggc
     2161 a
//
LOCUS       DAZGCQ010000267         2134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000267 DAZGCQ010000000
VERSION     DAZGCQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.270"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..785)
                     /locus_tag="ACHK07_005256"
     CDS             complement(<1..785)
                     /locus_tag="ACHK07_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105427.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLARHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTDP"
     gene            complement(782..1192)
                     /locus_tag="ACHK07_005257"
     CDS             complement(782..1192)
                     /locus_tag="ACHK07_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105428.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQATR
                     RVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSVAV
                     KGIYTAEDFIGEYQRLLMLYRAAFQAATAGECDA"
     gene            complement(1261..1488)
                     /locus_tag="ACHK07_005258"
     CDS             complement(1261..1488)
                     /locus_tag="ACHK07_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO8105429.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            1812..>2134
                     /locus_tag="ACHK07_005259"
     CDS             1812..>2134
                     /locus_tag="ACHK07_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105430.1"
                     /translation="MWRINITWQLVIVCIYGVFLMSDKLPSDYEILFDLWYSYWLERI
                     TCVFHRRVSAVLRISAFLVGVAAFVLCDFVVLVLFSVLLFLLWQCMAASFERRAEKAE
                     WQAVS"
BASE COUNT          395 a          545 c          666 g          528 t
ORIGIN      
        1 tcggggtcgg tgcggcggtc gggcctgatt tccagcgacg gccagcaggg tggcgtgata
       61 cccagtttta acgccatgtg tgccagtggc tggtaggtca gtaaccaggt gaacaggtca
      121 tcgtcacccc tgccggtctt ttcgatgacc tcatcacatg cgcgctccag tgcggcggaa
      181 aacgcggtgg cggtcagggt ggcaaggcgc tggatttcgt cacggctcag ctgcggcagc
      241 ataagcagtt catccagccg ttcgcgggcg gcggtggcct taaaggccgg ggtggcaaag
      301 cgttcattca cacgggcgat acgtttcagg cgggcacgca gcccgtgacg aacgaagtta
      361 tccgccgcgt atgcgctgtt gttcttaagt ccttcccggc gtgcgctctc ctgtttgcgg
      421 ctcaggcggg ccaggtcccg gatgagcgga ccggcaagaa acggcggcag ggcgcgcagc
      481 tctgccatgg cttcagatac cagtgtgggc ggattttcgc gtttgtgccg cgccagtttt
      541 tccagtctga tccgacgctc ttcgtcgtgg tgaagatgcg aattaattcg cttcccttcg
      601 tctgtggttg tcagaaaggc gacggtttct tcatccagtc tggcgtcagt gcgaatgtcc
      661 tcgttgatgt agctctgaat ggcctggcgt aatgcggttg gctgtgccct gccgaacgtt
      721 tcaggatcga taaccggcag cggggcgttc cacgggtacg ccagttgtgc gttatggtcg
      781 ctcatgcgtc acactccccg gcagttgccg cctgaaacgc cgcgcggtac agcatgagaa
      841 ggcgctggta ttcaccgata aagtcttctg cggtgtatat acctttcacg gccacgctgt
      901 gcgttgtcat ttccagcaca agcgtcatct ctgacgacga ccagctgatg gcggtgctgt
      961 cggttccgtt gatgtgaaat aaaccaaaac catcaccttt cacgtccacg cggtaacggt
     1021 cgtcaacggt gaaggagaaa tccccgtatg tggtgacgcg tctggtggcc tgtttacagg
     1081 cgcggcggta atagtcgtgc cagtcgttca ggcgggaagc cagccgggga tccactgccc
     1141 acatccagtt aaaaaagtcc tgaatatctg aaagtccctt aacgctcttc atgagaacct
     1201 ccggtaacag acgtgcggaa gcctcccgcg cacgggtgcg ggatggcttc aggatgttgt
     1261 ttagtggtgg tgttgctggt tgataaggtc ctgcagttct gccaggtcat ccgccagata
     1321 gctgaagacg gcggcacagt aacggtctga cagcgcgtgt gcgtgttcat gcagcatgtt
     1381 gatgtacatg atttgcgcga cgcgtgatgc gcgccagagt ctgcggttga tttcagtccg
     1441 ggtgtgaaga cgctcagcgc gggcgcgctg ttgcttgcgg tttgccatgg tgtggcctct
     1501 ttgctagtaa gttctgaaaa ctcaccaacc tgaggcggta atctcagggt ggcgagacgt
     1561 acagggttac cacaaccggc tagcaaagaa tccggcccga ccgaagtcgg ccccgtacgc
     1621 cccgccataa ttcgtgtgcg aaaaagacgt gacgatacgg tacgcacaaa aaaaccgctg
     1681 gcgcggttgt gcgctttgct agtcggcggg gtggtaatcc cggcacccgt tttatgaggt
     1741 gcgggaaaac tgtaacccga ccaggcgagc gctggcaagc ggtttttgtg ttgcgtgctg
     1801 gtatggttag aatgtggcga attaatatca cctggcaact ggtgattgtc tgcatatacg
     1861 gggtgtttct gatgagtgat aagctccctt ctgattacga aattcttttt gacctgtggt
     1921 acagctactg gcttgaacgt attacatgtg tgtttcaccg acgcgtttct gctgttctgc
     1981 gaatcagcgc ctttctggtt ggtgttgcgg cgtttgtcct gtgtgatttt gttgtgttgg
     2041 tgctcttcag tgtgcttctg tttctgctct ggcagtgcat ggctgccagt tttgaacgca
     2101 gggctgaaaa ggctgaatgg caggcagtca gtta
//
LOCUS       DAZGCQ010000268         2049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000268 DAZGCQ010000000
VERSION     DAZGCQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.271"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..138
                     /locus_tag="ACHK07_005260"
     CDS             <1..138
                     /locus_tag="ACHK07_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8105431.1"
                     /translation="SGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
     gene            148..1563
                     /locus_tag="ACHK07_005261"
                     /pseudo
     CDS             148..1563
                     /locus_tag="ACHK07_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1567..2007
                     /locus_tag="ACHK07_005262"
     CDS             1567..2007
                     /locus_tag="ACHK07_005262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105432.1"
                     /translation="MIIGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKI
                     QSRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKTINLGPLPVLNNCFSRTNMIFEACG
                     FNNPSMNTNAPISLQVLGELYGYQRYIMPKGPFTGFPFEFQEVK"
BASE COUNT          520 a          505 c          636 g          388 t
ORIGIN      
        1 tcagggatac ggcacattgc tattaatatt ttgacgaatg ataaggtatt caaggcaggg
       61 ttaagacgta agatgcgaaa agcagccatg gacagaaact atctggcgtc agtccttgcg
      121 gggagcgggc tttcgtaatc ttgccctggg ctggcgaccc gtcaggagcc ggacggtctg
      181 ccgccggtgg agtggctgac gtatggcagc ggttatcttg cggggatgaa gctgggcgga
      241 acgccgctgg tcgagtacac gcgggaccgg ctgcaccgtg agacggcccg cagcttcggc
      301 ggggaggcat atgaacttgc caccgcctgg aataccagcg gccagctcca gagcaggcac
      361 ctgaaccttc cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc
      421 atcagcggcc cgcaggagag ccgggagtac cgttacagtg acacgggaag gctgacgggc
      481 gtccacacca cggcggcgaa cctggatatc gatatcccgt atgcaacaga cccggcggga
      541 aaccggctgc cggacccgga actgcacccg gacagtacct tcacggcgtg gccggataac
      601 cgcatcgcgg aagatgcgca ctatgtctat cgctacgatg aatacggcag gctggcggag
      661 aagacggacc gtatcccgga aggggttatc cggatgcacg acgagcgcac ccaccactat
      721 cactacgaca gccagcaccg gctggtgttc tacacgcgga tacagcatgg cgagccactg
      781 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtgtggcgt
      841 cgcgggcgtg acctgacggg atggatgtcg ctgtcgcgta aaccggagga gacatggtac
      901 gggtgggacg gcgacaggct gacgacggtg cagactgaca ccacacgtat ccagacggta
      961 tacgagccgg gaagcttcac gccgctcatc cgggtcgaga cagagaacgg cgagcgggag
     1021 aaagcgcagc gccgcagcct ggcggagaag atgcagcagg aagggagcga ggacgggcac
     1081 ggcgtggtat ttccggctga actggtgcgg ctgctggaca gactggagga agaaatccgg
     1141 gcagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
     1201 caactggcca gacaggtgga gccggaatac acaccggcgc gaaaagttca tttttaccac
     1261 tgcgaccacc ggggcctgcc gctggcgctc atcagcgaag acggcaatac tgcgtgaagc
     1321 ggggagtatg atgagtgggg aaacctgctg aatgaggaaa acccgcatca cctgcaccag
     1381 ccgtaccgtc tgccagggca gcaacacgat gaggagtcgg ggctgtatta taaccgtcac
     1441 cggtactacg atccgttgca ggggcggtat atcatccagg acccgattgg gctgaagggg
     1501 ggatggaatc tgtaccaata tccgctgaat ccagtgcaac acgttgatcc attagggctt
     1561 agtactatga taataggtaa cggccccgtt cctgataatc cttttgggca tgctgcggcg
     1621 gcaaatcgat atggattaat gagttcggga actggggatg agatgggagc ctctgtctct
     1681 gattatttta aaaagataca atcccgcaga gatacctgga tttggataat agatacaaca
     1741 gatgaagaag aacaatgtat gatgaataag gcaatagacc ttaataaaaa actaaaaact
     1801 attaacctcg ggcctcttcc tgttttaaat aattgtttta gccgaacgaa catgattttt
     1861 gaggcgtgcg ggtttaacaa cccctctatg aatacaaatg cgcctataag cctacaagtg
     1921 ctcggtgagc tttatggata tcaaagatat attatgccta aaggtccttt cactggattc
     1981 ccatttgaat ttcaagaggt aaagtaaatg gaaatgcttg ggaagatatt agtgataatt
     2041 atttccatg
//
LOCUS       DAZGCQ010000269         2035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000269 DAZGCQ010000000
VERSION     DAZGCQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.272"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1673
                     /gene="mchF"
                     /locus_tag="ACHK07_005263"
     CDS             <1..1673
                     /gene="mchF"
                     /locus_tag="ACHK07_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO8105433.1"
                     /translation="VWPGSEFLAETQQTRISLRSLINSIYGIKRTLAKIFCLSVVIEA
                     INLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMSTLIN
                     IQWQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLDTLRATFTTCVVGAIMDSIMVVGV
                     FVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQISEETLVRGARASSYFMESLYGI
                     ATVKIQGMAGIRGTHWLNLKIDAINSGIKLTRMDLLFGGINTFVAACDQVAILWLGAS
                     LVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQLRIMSLHNERIADIALHEKEEKK
                     PEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLNLSVAPGESVAITGASGAGKTTLM
                     KVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDDRLFSGSIRENICGFAEET
                     DDEWMTECAKASHIHDVIMKMPMGYETLIGELGEGLSGGQKQRIFIARALYRKPGILF
                     MDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTLRTVDRIFRITN"
     gene            complement(1734..>2035)
                     /locus_tag="ACHK07_005264"
     CDS             complement(1734..>2035)
                     /locus_tag="ACHK07_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105434.1"
                     /translation="LALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADIAGSAIGGY
                     IGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT          554 a          386 c          494 g          601 t
ORIGIN      
        1 aggtctggcc cggaagtgaa ttcctggcgg aaacccagca gacccgcata agtctccgtt
       61 cactgattaa cagtatttac ggtattaaaa gaacactggc gaaaattttc tgtctgtcag
      121 ttgtaattga agcaatcaat ctggtaatgc cggtgggaac tcagctggtt atggatcatg
      181 cgattccggc gggggacaga gggctgctga cgcttatttc tgctggcctg atgttcttta
      241 tattgctcag ggccgcggtg agtatgctgc gtgcatggtc ctcactggtt atgagcacgc
      301 tcatcaatat acagtggcag tcgggtctgt ttaaccatct tctcagactg ccgctggcgt
      361 tttttgaacg ccgtaaatta ggtgatatcc agtcgcgttt tggctccctt gacactttga
      421 gggccacctt tactacctgt gttgttgggg caatcatgga cagtattatg gttgtgggtg
      481 tttttgtgat gatgctgtta tatggaggat atcttacctg gatagtgctc ggttttacca
      541 tggtttacgt tcttattcgt ctggtgacat acggctatta ccggcaaata tcggaagaaa
      601 ctcttgtcag gggggcccgg gccagctcct attttatgga aagcctgtat ggtattgcca
      661 cggtaaaaat ccagggtatg gctgggattc ggggaacaca ctggcttaac ctgaaaatag
      721 atgcgatcaa ttcaggtatt aagttaacca ggatggattt gctcttcggg gggataaata
      781 cttttgttgc cgcctgtgat caggtggcga ttttatggct gggtgcaagc cttgtgatcg
      841 ataatcagat gacaataggg atgtttgtgg catttggttc ttttcgtggg cagttttcgg
      901 atcgggttgc ttcgctgacc agttttcttc ttcaactgag aataatgagt ctgcataatg
      961 agcgcattgc agatattgca ctacatgaaa aggaagaaaa gaaaccggaa attgaaatcg
     1021 ttgctgacat gagcccggtt tcactggaaa ccactgattt aagctaccgg tatgacagcc
     1081 agtcagcaca ggtattcagt ggtctgaatt tgtctgtggc tccgggagaa agtgtggcta
     1141 taactggtgc ctccggtgcc ggaaaaacca cattaatgaa agtattatgt ggactgtttg
     1201 aaccagatag tggaaaagta ctggttaatg gcacggatat acgtcaactt ggaataaata
     1261 attatcaccg tatgatagcc tgtgttatgc aggacgaccg gctattttca ggatcaattc
     1321 gtgaaaatat ctgtgggttt gcagaagaaa cagacgacga atggatgaca gaatgtgcca
     1381 aagcaagtca tattcatgat gtgataatga aaatgccaat ggggtatgaa acgttaatag
     1441 gtgaactggg ggaaggtctt tccggcggtc aaaaacagcg tatattcatt gcccgagctt
     1501 tataccggaa acctggaata ttatttatgg atgaggctac aagttctctt gatacagaaa
     1561 gtgaacgttt cgtgaatgct gctataaaaa agatgaatat cacccgggtg attattgcac
     1621 atagagagac aactttaaga acagttgatc ggatttttag aattacaaat taggataata
     1681 tatcactaaa agcagcattg ctgcataata agctatgctg ctttagaaat taatcaactt
     1741 ccactactag aagaggatgt tgatgagtta aatcctgagt ttaataaacc tccattgtag
     1801 tttggattaa aacttccaag tcctataccc ggattatagt taataccgtt tgatggtatt
     1861 gttattgtcc tatttgaacc atcagcaata actcctccaa tatatcctcc aattgcactt
     1921 cctgctatat ccgcaccagg gatttttgtt gctttaccta aaaaaccacc agccgcagta
     1981 cctattgcaa cggatgcatc tttatctaat cctgcaccat gtattaaagc aagtt
//
LOCUS       DAZGCQ010000270         2020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000270 DAZGCQ010000000
VERSION     DAZGCQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.273"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1123)
                     /locus_tag="ACHK07_005265"
     CDS             complement(<1..1123)
                     /locus_tag="ACHK07_005265"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8105435.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFALESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILTSEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGASGSRIVVSLLYQM"
     gene            complement(1133..1852)
                     /gene="fabG"
                     /locus_tag="ACHK07_005266"
     CDS             complement(1133..1852)
                     /gene="fabG"
                     /locus_tag="ACHK07_005266"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO8105436.1"
                     /translation="MRNKVCIVTGAAQGIGRKIVEDFSKSEVKRVYACDIKHTDKSYP
                     ENVTPVILDVCDRDSINTVISKIYSDEGRIDVLVNNAGVTRDNLINNMTEQDWDIVID
                     INLKGVFNITQAVAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKGWA
                     KEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVLFL
                     ASEKSSFITGQTLKIDGGLVI"
BASE COUNT          608 a          467 c          282 g          663 t
ORIGIN      
        1 tcatttgata taataaagaa acaacaattc ttgagccaga tgctcccaaa ggatggccta
       61 gagctatcgc ccctccatta acatttgttc tttgatttag ccaatcaata ctaactgagt
      121 gattttcaga aatacttttt aagaccccta ttgcctgagc cgcaaacgcc tcgttaattt
      181 caaaaagatc aatatcttta atatccagtt ttgctttttt taatgcatta tctattgctg
      241 gaacaggccc cagccccatc actgatgcat caacgcctcc ttgtccaaat gagcgaattt
      301 cagccaaagg ttgtaattcc aatgtctgaa ttgctttctc tgaagtaaga ataagagctg
      361 ctgcaccatc gtttattcca gatgaatttc ctgcagtcac ggtaccattt ttttcaaatg
      421 ctggacgtaa cacagaaagt ttctctaatg aggagtctct acgaggatac tcatctgtag
      481 taacttcaat atttcctttt cgggtatgta cttgtaccgg agtaatttca tcctcaaatt
      541 ctccattgtc aatggccttt aaggctctct tctgactttc aagcgcaaat gtatcctgtt
      601 catatcttgt aatattgaac tgtcgggcta tattttctgc tgtaatcccc atatgatagt
      661 gattaaaagc atcagtaaga ccatctgtaa taagagagtc actcattacc tgattaccca
      721 ttcgtacacc acttcttgca tgatgatcaa tcaagtaagg tgcctgactc atattctcta
      781 tgcctgccgt aacaacaaca tccatatctc cactacgaat tttcattgca gcatcgataa
      841 tagttttcat accactacca caaatcatat tcaaagtcag agccggagtt ttccatggta
      901 taccggcttt aacagcagct tgtctacctg gccccattcc ttgtccagca gacaaaacat
      961 tcccgacaat aacttcatca atacattcaa cgtttattct gctctgttta attgctgcag
     1021 atatcgcaat tgctccaaga tctgaagctg acactgttga taacgaaccg ccaaaacatc
     1081 ctataggcgt tctttttgcc cctgtaatat atactttttc cattcctcct ccttaaataa
     1141 ctaatccacc atcaattttt aatgtttgac cagtaataaa agatgatttc tcactagcta
     1201 aaaaaagaac accattcgaa atatcctcag gtgttgccat tcgccctaaa ggggttttag
     1261 ctatcatatg gttaatgact ttctctggca gattttctgt catcggggta ataacgaatc
     1321 ctggagctac acaattcacg cgtactttag caccttttcg ggaaaattct ttagcccaac
     1381 ctttagacat tgcaataaca ccacctttag ttgcggcata attactctga cctatattac
     1441 catcagttcc tacaacagaa gacattgtta tgattgcccc atgattttgt tccatcataa
     1501 taggtgcaac tgcctgagtt atattaaaaa cacctttaag gtttatatca attacaatat
     1561 cccagtcttg ctctgtcata ttattgatca gattatctct tgtaactcct gcgttattaa
     1621 ctaaaacatc tattctacct tcatcagaat atatcttact aattactgta ttaatcgaat
     1681 cccgatcgca aacatcaaga attaccggag tgacattctc tggataggat ttatcagtat
     1741 gttttatatc acatgcatat actcttttta cctctgattt agaaaaatcc tccacaattt
     1801 ttcttcctat accttgtgct gctccagtaa ctatacatac tttattacgc atttttattc
     1861 cctcattttt gaatatagaa caggatgcct ttcgttttgc aacaaaacat cttttaacca
     1921 ttctcgttca taaagtaaaa ccttaaaaat acagaatcac aaaaaaaaaa cacttataac
     1981 actcacgcaa caatcaaaaa atacattatt tcccaatgag
//
LOCUS       DAZGCQ010000271         2011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000271 DAZGCQ010000000
VERSION     DAZGCQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.274"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..334)
                     /locus_tag="ACHK07_005267"
     CDS             complement(101..334)
                     /locus_tag="ACHK07_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105437.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(423..1046)
                     /locus_tag="ACHK07_005268"
     CDS             complement(423..1046)
                     /locus_tag="ACHK07_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8105438.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            complement(1135..1644)
                     /locus_tag="ACHK07_005269"
     CDS             complement(1135..1644)
                     /locus_tag="ACHK07_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105439.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          552 a          544 c          424 g          491 t
ORIGIN      
        1 atgcggccga tgtacaactt cctgggaaaa ttcaaacccg aaccccggaa agaaccccct
       61 gccggcaatc acactgcctg atggcagcat aatccactta ttaattctca tccccggaga
      121 caagcccaga aaaccactcc gggtgttcac tgtatgccgt gttcagcgca tcccaggaaa
      181 cgttgatata aaagctgtcc tgcaatttgc gggtaacaat ttctgccacc cggttacggt
      241 tatccgcctg tccggcataa cctctctctg ccagccactc attcagttca tgttccggag
      301 acagattgat aatatcgtaa tcggaccttg ccattcccgt ttccttagca catgttttca
      361 ggctcgggga taaagaatgt cgtactgctg ctgccagagc aatattcccc ctgatggtcg
      421 aatcagagat agtaggccgg tactttgcgg gttgttcccg tcagattatc atgcacctcc
      481 gcggtcgttg tcaccaccgg cattccgctc tttctcaacc tcaggacatc agccgcaata
      541 cgctgactgc cgaaccagaa caggccgtcc agtgcagtca ccagcaaccc cgcctccagc
      601 gcatgcttca gccgttcacg gggcgctttc acttcccggg caatctgctg gtatggcgat
      661 gatgtgtttt cattcccaat cacccggcga atacgatgag acagatgata ccggtatgta
      721 tccggcacac cggaaaggct ggccttcagg ctgtacacgc agccaaagcg tttatcgttg
      781 aacaccacat ttttctggct gatatcccat tcttcacgca gcgcggcaat cagttgtggt
      841 gtccgggtaa gcaacaagcg aaaaggcagt tcaaaactgg tgacgtaatc cacattcagc
      901 agtgcaatac gcagccgttc ttccggcccg gcctctgtct gccggcactc ctccagaaca
      961 tcctgccact gctggtgaag gcgtggaagt tcattcagtt cagtaaaact gtatttatcc
     1021 gcaagatagt caattcgtgt acgcatactg aaaagaattc cgtataaaga ttcagctggc
     1081 aaaactttat cagtctgtaa aaactaacgg aagagtcgat atttctcccg tccatcaccg
     1141 gatgattgtt gcaatacccc gtggcatcag agactgaaca gccgttttta acgccacgta
     1201 ttgccctgat gaaccaggcc gggcaacccg aaaacatctt tccacccggc attgtccgcc
     1261 agcacttatc accgaccagg tctgttgcag taaatccacc agttgcgcac attcttcatc
     1321 agccatgacg ccgactcgcg gatggcaaat actcgtctgg ctgagcagca acagacatcg
     1381 tatctgtgct ccttcatgtt gttcccgcaa ccagcgtaat accaccggcg ctgatgggct
     1441 gaccgtgtga ttatgctccg taatacgttc gtgtacccgt cggtgaaagg aactacaaaa
     1501 tgtctgaatc tgttgaccat agattccttc tgccgaatag agcgtgaagt gaaatgccaa
     1561 cagatttggc cagtggtcca ccgcctgctg aacaaaacgc cggatttccc ccggctctga
     1621 aattaaggct tcggttattt gcacgaaatg ttccttttca acccctgttg aattttgtca
     1681 agccggtgca gacacagtaa agcgcgcatc aacacctgaa aggtacgata caaacacaag
     1741 ttacaaacaa taagaacgga aggacaatta atcagtgagg aataataact aaattaacca
     1801 aaagaatgtt actgacaata ctaccaatac ttaaatagca atatattaat taatgtaaga
     1861 acagatgccc ggtaagacat atacacaata catccacaaa cacactcttt tcattaaatc
     1921 aatcacctga ttaaaccatg aaagtgtgat gtaagtcaaa attactgaca ctatcaattg
     1981 ataatgatta tctctatcat tatcgtgaaa t
//
LOCUS       DAZGCQ010000272         1985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000272 DAZGCQ010000000
VERSION     DAZGCQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.275"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHK07_005270"
     CDS             44..577
                     /locus_tag="ACHK07_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105440.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCAQINAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIDR"
     gene            798..911
                     /locus_tag="ACHK07_005271"
     CDS             798..911
                     /locus_tag="ACHK07_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105441.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            913..1407
                     /locus_tag="ACHK07_005272"
     CDS             913..1407
                     /locus_tag="ACHK07_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8105442.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIKLSFYY
                     PEDI"
     gene            1404..1628
                     /locus_tag="ACHK07_005273"
     CDS             1404..1628
                     /locus_tag="ACHK07_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105443.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          498 a          435 c          573 g          479 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgct gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taaccacaca acagcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccgggtggat ggtaagcctg tgattcctgg
      241 catgaagctg tcgaaggaaa aatgcgcaca gattaatgcc attgaacgtg ataaggcgct
      301 ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa cctcagaaag cgggtattgc
      361 gtcattctgt ccttacaaca ttggccccgg taagtgtttc ccgtcgacgt tttataaacg
      421 aattaatgca ggcgatcgca ggggagcatg tgaggcgatt cgctggtgga ttaaggacgg
      481 tggcagggac tgccgtattc gctcaaataa ctgctacggg caggttatac gtcgtgacca
      541 ggaaagcgcg ctgacgtgct ggggcatcga cagataagca gaatattttg ctgaaaaatg
      601 acgttgacca acgcgaacgg ataacacgaa atcctgcgaa ctggcaaaat ctaagtgaat
      661 aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt gtgtttctga ccttggataa
      721 ggcaagggag aaattatggt gagggaggtg ctttccctgt gaggaagtat aaaagattct
      781 ttctgaggtt gtccattatg aaaggcattg aagtggagac gccagccagt ctggatttaa
      841 caagagcggc agcttttgcc attcgtatta tagccattgc tgttctgatc tgggcaatcc
      901 gttggtggtg aaatgaaccg cgttctgtgt gtggtcatca ttgctctgct ggtggcctgt
      961 ggtgcgctta gtctggggct gaatcattac cgcgataacg ccatcactta caaagcgcag
     1021 cgcgataaaa aagccagaga gctggagctg gcaaacgcaa ccattactga tatgcaggtg
     1081 cgccagcgtg atgttgctgc gctcgatgca aaatactcga gggaattagc cgatgcgaga
     1141 gctgaaaatg aaactcttcg cgctgacgtt gccgctggtc gtaagcgcct gcgtatcaac
     1201 gccacctgtc caggtcccgt gcgtgaagcc accggcaccg cccgcgtgga taatgcaacc
     1261 ggcccccaac tggcagacac cgttacacgg gattatttca ccctcagaga gcggctgatg
     1321 acgatgcaga agcaactgga aggggcgcag gaatatatcc gtactcagtg cattaagttg
     1381 tctttttatt atccggaaga tatatgaaga aattactggt aaccgtaaag ccttttcagg
     1441 gaacaattcc gttccgtgtt ttgcagcctg gtcgtgttct tgttgaaggt acgttcagtg
     1501 gtaaatgtac gcaatcatac tcccgggtct ttcgggtgga tgcctcgaat gaagagctaa
     1561 ccgtggagtg tgctgtgaat gccgctaaat gccgcatggt atcggctgca ttacagccag
     1621 tgtgttgagc gaccttatta tccatggcgc ggtattgtcg ccgtattccc gtattaacag
     1681 agaccgcagc ccgacaggga gactcctctg cgcgagtgtg cggggataat caaaaacgat
     1741 acacaccggg gtttaccgcg ttaacggagc gcggcgttgt cccctcatgg tcgctggtcc
     1801 ggtgcgatgg tggaagaaac cggacgatgt gttacctcgc atgacctgtt atgtcatgtg
     1861 tctgatttgt gatttaagtc ggataattgt cgttgccatt aagcagagga ttgatggccg
     1921 acagggtggc attgttagaa taagacttat tcttatctgt gccgggaatg aaaaagaaaa
     1981 gaaat
//
LOCUS       DAZGCQ010000273         1887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000273 DAZGCQ010000000
VERSION     DAZGCQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.276"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHK07_005274"
     CDS             complement(<1..881)
                     /locus_tag="ACHK07_005274"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8105444.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSLPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1887)
                     /locus_tag="ACHK07_005275"
     CDS             complement(871..>1887)
                     /locus_tag="ACHK07_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8105445.1"
                     /translation="DRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPSMLHIFEP
                     VEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAG
                     APQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGDGLKLQTAQDSDNGFSALE
                     QALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFS
                     CWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIDSGL
                     STYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETT
                     PRET"
BASE COUNT          429 a          606 c          468 g          384 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc tgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccggca
      301 tagctgacat ggctcatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatgtcc agcagtacgc cccggacaga ggtgtctgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 ataccaccca gccggtgcac cagcgtgccg gtcaccggca gtaccgcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttacct ccggccatgt actcgtccgt ttcagccagc
      721 atcccgggag catcaagctg tacctgctgt tgtggtaacg aaagacttgc tgcccccatc
      781 tcgcgcccga gcgcgcaaaa gaaaacccgc gcataggcag gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcgccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttcctgcgc caatccactc
     1141 tgcccgtgac caggctgaac gcgcctgata aaaatcaaaa cgcgcccgtg gaggacgaat
     1201 aatcccccgc agaagtgcct cttccagcca gcaggaaaac atctgcgtgg ccagccgggc
     1261 cgcaataaat tttcgtcgtc ccataaaata gcgccacgac tcattggcgg aggcgcgggc
     1321 acttgaataa ctgaccttcg agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc
     1381 ggcggcgata taccgcagca gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg
     1441 cgcggtctga agtttcagac catcaccggg gaaaaggtgc ggaattttga caccgcccag
     1501 cgtcacatga ttcgtgtcat accagctgga gaacttctcc agaatattaa taagcggatt
     1561 atcctgctga ccctgcggtg cccccgcgat atattcaaag gccttttcgg tatcaagttc
     1621 actttcaatc gtcgctgcat acatggcttt aacaatggcc gactgaagct gtgttgcctg
     1681 cagggaatca agcatcttca gccgttccat gacactgtaa aactgattgg ccccacgggt
     1741 ctgcccgtcc tccaccggct cgaaaatatg cagcatggac ggacgcccgg tgggaagttc
     1801 acgcgggatc cgttcccatc gtccactacc agagaacgga aaatcatcct cacagatatg
     1861 gtacgcaacg gcacggccat atcgatc
//
LOCUS       DAZGCQ010000274         1857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000274 DAZGCQ010000000
VERSION     DAZGCQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.277"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..985
                     /locus_tag="ACHK07_005276"
     CDS             <1..985
                     /locus_tag="ACHK07_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8105446.1"
                     /translation="YRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVK
                     QLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIA
                     KKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVA
                     RVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVG
                     NSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPE
                     EDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1300..1722)
                     /gene="subB"
                     /locus_tag="ACHK07_005277"
     CDS             complement(1300..1722)
                     /gene="subB"
                     /locus_tag="ACHK07_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO8105447.1"
                     /translation="MTIKKFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(1739..>1857)
                     /locus_tag="ACHK07_005278"
     CDS             complement(1739..>1857)
                     /locus_tag="ACHK07_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO8105448.1"
                     /translation="SLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMNEEL"
BASE COUNT          542 a          384 c          425 g          506 t
ORIGIN      
        1 gtatcgtcat gattttaatg aaaaaataga tttctggtat gtgatagccc tcgcaggaca
       61 gtctaatggt atggcctacg gtgaaggtat tcccctgcca gatacactcg acaaacccga
      121 gtcccgtgta aaacaactgg ctcgtcgaaa aacaatcaca ccaggaggaa aagagtgtaa
      181 attcaatgag attattccgg cagatcactg cctgcatgat gttcaggata tgagtggata
      241 tcaccatccg gctgctgatt tgcataaagg ggaatatggt tgtgtcggac agggattgca
      301 tatagctaaa aagttactgc catacatacc tgagcaagca gggatattac tggttccttg
      361 ttgcagagga ggtgccgcct ttactgttgg agctgaaggt atgtatgtac ctgatacagg
      421 ggcaacagct gatgcaatgc ggtggggcac cggaacagca ctctatgagg acttagtagc
      481 ccgggttaag gtagctctgg aatataacag aaagaataaa ctgctatctg tctgctggat
      541 gcagggggag tttgatctta tgtcaccaga ttatgaaaag catcctgact tgttttatca
      601 gatggtaact tctttcaggt ctgagttatc tgaatactca tctcagtgtg ttggtaattc
      661 gtcagaaaga gttccatggt tgtgtggtga tacaacctgg tactggaagg aatcttatca
      721 aaaagaatat gattttatat atggacatta cagacaacgg actgatgatg aaatacattt
      781 tctcagcttt caggatagta accgacatga attaactaat gagccagaag aagatgccga
      841 tgatttatct gtcgggtatc tggggtcatc atggaggaca gaattaagct ggacaacgtc
      901 acagagaagt acacacttta attcaatggc cagaagggga gttattgctg aatgttatgc
      961 gcaaaaaata agaaattatt tgtagacaag ttcaggatat atctacagct ctgacatgat
     1021 tctctgagag acatgacgga gtggtatgga gttgtcggta tcaacaccag gatagctggt
     1081 cgttaccgtt tgatatcact ggtgctgata ctatttggac ggtagtgtgt cacgaagaca
     1141 cgcaccaggg acgtgagtcc ctgtaatatc atcgacagtc ccattatggc atgtctgcac
     1201 ctacaacgtc atttcgaggt cggctcagtg ttcactcgag tcttatatca ctgacgccga
     1261 cactccatcc agagtatctc tggacggaaa catcacctat tagttctttt ttctgtcggg
     1321 gccaaaacat tcagtcgatg tggcgcaggt cgataatcca acaagggcat tggacgttaa
     1381 tgccttcaca aagggtggat acgtccatac cccgggttca taataaatcc tgaccaactg
     1441 acctgttgtg taaaaatata gtgcctgatt atataatgtg gaaaacgaag gcccccagac
     1501 agatgaattc ttcatcgagc aggctcttat ggaagaacca ccaggttgtt tcccttcaat
     1561 acaaaaataa gggttattat ctatttgccc tgtatgaaac tgatcaataa caacgcctga
     1621 aaacatgcca tcccgggcat cgccagtcca ctctgccatg gcaggattaa gggataaaca
     1681 tcccatgaca cctgcacata caaaaaactt cttaatagtc atattcacct ccgaagtttt
     1741 acagttcttc attcatcctt ccctgacgga caggaatata atttttcttg cagaacatgc
     1801 taattgcttt ttctgcatta agaaccctcc cttccgtcac cttatctgca agtgagg
//
LOCUS       DAZGCQ010000275         1855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000275 DAZGCQ010000000
VERSION     DAZGCQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.278"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..1255
                     /gene="ompC"
                     /locus_tag="ACHK07_005279"
     CDS             155..1255
                     /gene="ompC"
                     /locus_tag="ACHK07_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8105449.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGKDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1419..>1855
                     /locus_tag="ACHK07_005280"
     CDS             1419..>1855
                     /locus_tag="ACHK07_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO8105450.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKL
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSFNKINTITFPNESNLENIYIDHNNLESLDLKN"
BASE COUNT          570 a          367 c          364 g          554 t
ORIGIN      
        1 tcgatcacat atcgataaat aatattattt catctatttt tctcattgtt tttaatcacc
       61 attctaattt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
      121 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
      181 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
      241 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
      301 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
      361 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
      421 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
      481 ctctctggac tatggccgta actacggtgt cggctatgac attggttcat ggactgatat
      541 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
      601 caccggtgta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
      661 tgccgcgcaa tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga
      721 ctacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
      781 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgctgc
      841 taatcctctc aatgcctccg gtaagaatgc agaactgtgg gccacaggta taaagtatga
      901 tgccaacaac atctactttg cagctaatta cgctgaaaca ttaaacatga ccacctatgg
      961 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
     1021 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcaaagatc tgggccgcta
     1081 cggcgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
     1141 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
     1201 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacatcc
     1261 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
     1321 aagtaaaaat gagcaatttt tgatatttct ggtcattcac ctcaattaat attattccat
     1381 accccccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
     1441 cagcttgtgt aaaaaataat gccagttatc aatttaataa cgcattacct aacaaagaaa
     1501 ccatctccag caatttttgt gaacgactag aacaatgggg taataagttg cttaataatg
     1561 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
     1621 ctcttgattt gtcatatctt gatttgagtg agttaccgcc tatcccctcg acagttaata
     1681 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt tactgacaat gccagtctcg
     1741 ttaatatcaa cctcagcttt aataaaatta acacgataac ctttccaaat gaatcaaatc
     1801 tggaaaacat ttatattgat cataataatc tggaaagttt agatttaaaa aatca
//
LOCUS       DAZGCQ010000276         1803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000276 DAZGCQ010000000
VERSION     DAZGCQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.279"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..793
                     /gene="nanR"
                     /locus_tag="ACHK07_005281"
     CDS             59..793
                     /gene="nanR"
                     /locus_tag="ACHK07_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8105451.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(829..1764)
                     /locus_tag="ACHK07_005282"
     CDS             complement(829..1764)
                     /locus_tag="ACHK07_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8105452.1"
                     /translation="MGFIMNKDKSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPE
                     FLSQGYTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFS
                     AGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLG
                     DNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHL
                     YEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          633 a          370 c          304 g          496 t
ORIGIN      
        1 tattaaaaga aagtgttaaa ttaaaaactc atttgctcta atccatataa atcaatatat
       61 gtccgacaac aaaaaaaccg tgttacccgg gctaacaacg accagaaagc ccattgttac
      121 aaaaaaactt tcagaaatcg ttgccgaaga gcttgaaaga atgatccgcc aggaggagat
      181 aagggaaggt gaatacctgc cttctgaacg tgaacttatg gcattctttg gcgttggaag
      241 accatctgtc agagaggctt tatcatctct cagacataaa gggctaatcc ggtataacaa
      301 gggagaaaaa gccagtgtct gccgtccatc accagaaacc atcattaatg ggttatcagg
      361 cattgttaaa gattttttat ccacagctga tggcatcaag gattttgaaa gactacgcat
      421 cttttttgaa accaaccttg taagatatgc agcaaaaaat gcaacgcctg aggatattca
      481 caggcttgag aaggtgctca gtatccatca taactcactg agaacccccg aagtgtatgc
      541 tcaatatgat attgaatttc atagtatact tgctgatata cctgacaata aagtattaaa
      601 aacaatacac tcagcatttt ctgaatggct gataatggcc aaacctctgg aaaagaataa
      661 aatgacatat gaaaataaca aaaaaaccca tcagcagcat attgaaatac ttacggccat
      721 aaaagagcat gattgtgata aagctgaaca gctaatgtat aatcaccttt ataaacgaat
      781 tgaagcatca tagttactcg acgcttcacc aggtagtcac cataaatatt atttaaatcc
      841 atgagaatac atccagtcca taaacagttt tatccatcct ccaacaggaa gatctgtggc
      901 ccactgaaca ctaaagccat gccctccttt ctcatagaga tgcatttcag cagaaacgcc
      961 tttctctatt aggccacggt acatgagcag actgttttca attgacacaa gatcatcatc
     1021 acaacaatgg agtaaaaaca caggaggaaa acatgatgtg acattaagat ccgcactata
     1081 ttgtattata ttttctgagg tactattgtc ccccagtaat tgcttcctgg agcctggatg
     1141 tgtcacatca tctttcattg atattacagg ataaattaac ccgacaaaat cgggaatagc
     1201 gaaaatctct tgttctttac tgtcagaaga acgtttgaaa tcacaaatct gattcacaaa
     1261 cgttgaggcg atatgccccc cagcagaaaa tcctaataca ccaatacgat gacgctgtag
     1321 atgccattta tcagcattct tacgaatata aagcattgca gatattgcat caataaaagt
     1381 tttgtttata ttttcttcct cagctggaga tggtaatctg taactaagaa caaataatgt
     1441 atagccctga gataaaaact ctggagcaag agctgtacct tccttatcta acataacagc
     1501 ctggtagccc cccccgggaa taatcaatat tcctgttcca tttggtttat ctggtaaaaa
     1561 aataattaac tgaggagcat aaatatctgt caatacacga tcaagttcaa attccccgtt
     1621 atgttcagga tcctgataaa tacggcctga aagagattta ctctcagata ttgtctcact
     1681 tgacaagtca acaatagttg atttattaaa caagcttaac atcatttcat ttaaagattt
     1741 atctttattc attataaacc ccattaaaat aactcaggtg actgaacaac taatacagtc
     1801 acc
//
LOCUS       DAZGCQ010000277         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000277 DAZGCQ010000000
VERSION     DAZGCQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.280"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..464
                     /locus_tag="ACHK07_005283"
     CDS             <1..464
                     /locus_tag="ACHK07_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105453.1"
                     /translation="GGCVIEVMGTYEAAIVRVTIGEYGATGFINGTVCTCTVYGDTQR
                     FHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRFPVA
                     FPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            474..698
                     /locus_tag="ACHK07_005284"
     CDS             474..698
                     /locus_tag="ACHK07_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105454.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            698..1369
                     /locus_tag="ACHK07_005285"
     CDS             698..1369
                     /locus_tag="ACHK07_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8105455.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
BASE COUNT          449 a          348 c          551 g          391 t
ORIGIN      
        1 tgggtggctg tgtcattgag gtgatgggaa cgtatgaggc tgccatagtc cgggtcacca
       61 ttggcgaata cggtgcaacg gggtttatta atggcacggt ctgtacctgt acagtttacg
      121 gagacacaca acgttttcac tggcgggtgg attacagcac aaaaaacaag cccacaacag
      181 caagcctgac agtaaatggc tgggagcgtg atgaagtaac cggtcgtttg cggcaatggg
      241 gcagtattga ggtaagtgag gatgatggta agcttatgac acagcactct atccgttttc
      301 ctgtcgcttt tccggttgct gctctgaatg ctcaggtgtc tgcagtggga gacccccacg
      361 ccattaagat gtataccctg tcgaatccgt cattagtgtc tgtgactctg ataacagtca
      421 ggggatgcta cggcaagttt tcatgggaag cgatcggata ctgagggcat tgtatggaac
      481 agtatgtttt ttcaccgtca gagaatatgt tctatcccct gtccctgcgg ccggtttatg
      541 aggcagcagg gcgctggccg gaagacggta ttgtggtgga ttatgtggtg tataaagtct
      601 ttgcagcgga tgctgcaccg gcaggtatga agcggggcgt cggtgcggaa aaaatgccgg
      661 tctgggtgcc ggtatcggag gagggcacgg aaacatgatg catatcagaa atttttcgta
      721 ttacaccccg gcggagccgg atgttgcagg cgcgatgtac ctgaagtctg aggatggtca
      781 ggactggtat gagtgtcagg cgttgttttc gccggagacg ctgaaggtgg tgtatgacag
      841 ccggggtgtg attacgggat acggtaagga cactgcgtta ctgtggccgg tgaaccagag
      901 tgtggcagaa gtgccggata ccccggagag ccggaaaatt gacctgagcg ggcgctgggg
      961 ttttgacggg gagaaaatca cggacctgct gaccgcggag aaagcgcgag ggatgaaggg
     1021 cgatgaaatt aacgcctggc gtaatgcgat ggaagcggct aactacacgt ttgagcacaa
     1081 tgggcgtaaa tgggactacg ggaagtcaac gcagacgcgt cttgagccgt cggtggcggc
     1141 agcgaaagcg gggaaactgc cggaggcgtt tttctggacg gatgcggaaa acaatgatgt
     1201 ggaagtgaca gcagaagagc ttatagcgct gagtgaagcg gcagagcagg cgatgttcac
     1261 caaagggatg gaaatccacg tccggcagcg caccatgaag aaggagctgg aaaagctgac
     1321 cagtgcggat gagatactgg catacagggt tggctgggca caggaataac agacaaaaag
     1381 atgggggacc atcacctccc ccatgaactg agtcgattat ctaattgttg tgaagagact
     1441 ttacctacac ggatgcagag tgccacggaa aagttaatcc tgaaagtgat accggtctca
     1501 tttccagagc atcctgttga tatggataat ttttcagatt tccggaagtg agagttttgg
     1561 tgtttccata ccagccacag ttgatgtgtt ttatggcgcc tggcagtgcg attaagggaa
     1621 cagggataat gaaaaaacgg gggtatcacc acccccggta caaggaagta ttaactataa
     1681 tggaaacatt aatgaagatc tttagtgcta cgttatatgg cgaataaaga ctgccacag
//
LOCUS       DAZGCQ010000278         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000278 DAZGCQ010000000
VERSION     DAZGCQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.281"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1677
                     /locus_tag="ACHK07_005286"
     CDS             <1..1677
                     /locus_tag="ACHK07_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFO8105456.1"
                     /translation="IALEVWPGSEFQAETQQTRISLRSLINSIYGIKRTLAKIFCLSV
                     VIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMS
                     TLINVQWQSGLFDHLLRLPLAFFERRKLGDIQSRFDSLDTLRATFTTSVIGLIMDSIM
                     VVGVFVMMLLYGGYLTWIVLCFTTIYIFIRLVTYGNYRQISEECLVREARAASYFMET
                     LYGIATVKIQGMVGIRGAYWLNMKIDAINSGIKLTRMDLLFGGINTFVTACDQVVILW
                     LGAGLVIDNQMTIGMFVAFSSFRGQFSERVTSLTSFLLQLKIMSLHNERIADIALHEK
                     EEKKHEIEIVAHMGPISLETNDLSYRYDSQSAPIFSALSLSVAPGESVAITGASGAGK
                     TTLMKVLCGLFEPDSGRVLINGIDIRQMGINNYHRMIACVMQDDRLFSGSIRENICGF
                     AEEMDEEWMVECARASHIHDVIMNMPMGYETLIGELGEGLSGGQKQRIFIARALYRKP
                     GILFMDEATSALDSESEHFVNVAIKNMNITRVIIAHRETTLKTVDRVISI"
BASE COUNT          491 a          312 c          428 g          503 t
ORIGIN      
        1 attgcacttg aggtctggcc cggaagtgaa ttccaggcgg aaacccagca gacccgcata
       61 agtctccgtt cactgattaa cagtatttac ggtattaaaa gaacactggc gaaaattttc
      121 tgtctgtcag ttgtaattga agcaatcaat ctggtaatgc cggtgggaac tcagctggtt
      181 atggatcatg cgattccggc gggggacaga gggctgctaa cgcttatttc tgctggcctg
      241 atgttcttta tattgctcag ggccgcggtg agtatgctgc gtgcatggtc ctcactggtt
      301 atgagcacgc tcatcaatgt acagtggcag tcggggctgt tcgatcatct tctcagacta
      361 ccgctggcgt tttttgaacg ccgaaaatta ggtgatatcc agtcacgttt tgactccctt
      421 gacacattga gggccacatt taccaccagt gtgatcgggt taataatgga cagcattatg
      481 gttgtcggtg tttttgtgat gatgctgtta tacggaggat atctcacctg gatagttctc
      541 tgctttacca caatttacat ttttattcgt ctggtgacat acggcaatta ccgacagata
      601 tcagaagaat gtcttgtcag ggaggcccgt gccgcctcct attttatgga aacattatat
      661 ggtattgcca cggtaaaaat ccaggggatg gtcggaattc ggggagcata ctggcttaat
      721 atgaaaatag atgcgataaa ttcgggtatt aagctaacca ggatggattt gctcttcgga
      781 ggaataaata cctttgttac cgcctgtgat caggttgtaa ttttatggct gggagctggc
      841 cttgtgatcg ataatcagat gacaatagga atgtttgtag cgtttagttc ttttcgtggg
      901 cagttttcgg aaagagttac ctctctgacc agttttcttc ttcagctaaa aataatgagt
      961 ttacacaatg agcgcattgc agatattgca ttacatgaaa aggaggaaaa gaaacatgaa
     1021 attgaaatcg ttgctcatat ggggccaata tccctggaaa ccaatgattt aagctaccgg
     1081 tatgacagtc agtcagctcc gatattcagt gctctgagtt tatcagtagc tccgggggaa
     1141 agtgtggcta taactggtgc ttccggtgcg ggaaaaacca cattaatgaa agtactatgt
     1201 ggactatttg aacctgatag cgggagggta ctgataaatg gtatagatat acgccaaatg
     1261 ggaataaata attatcaccg gatgatagcc tgtgttatgc aggatgaccg gctattttca
     1321 ggctcaattc gtgaaaatat ctgtggtttt gcagaggaaa tggatgaaga gtggatggta
     1381 gaatgtgcca gagcaagtca tattcatgat gttataatga atatgccaat gggatatgaa
     1441 acattaatag gtgaacttgg ggaaggtctt tctggcggtc aaaaacagcg tatatttatt
     1501 gcacgagcct tataccggaa accaggaata ttatttatgg atgaggcaac cagtgctctt
     1561 gattcagaga gtgaacattt cgtgaatgtt gccataaaaa acatgaatat aaccagggta
     1621 attattgcac acagagaaac aacgttgaaa actgttgaca gagttatttc catttaaaac
     1681 ctactgacat aaccatagag gaattgcaac tgtatgagga atatatcttc ctgt
//
LOCUS       DAZGCQ010000279         1727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000279 DAZGCQ010000000
VERSION     DAZGCQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.282"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHK07_005287"
     CDS             <1..153
                     /locus_tag="ACHK07_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO8105457.1"
                     /translation="RLTPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTF
                     LGSTNF"
     gene            167..1588
                     /locus_tag="ACHK07_005288"
     CDS             167..1588
                     /locus_tag="ACHK07_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO8105458.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
BASE COUNT          416 a          383 c          581 g          347 t
ORIGIN      
        1 agactgacgc cttcagtctc cacgtcctgc gcagtcttcc ctgacggaga gcatgaagcg
       61 gctgaaacag gtaatgctgc ctttgccctg tggcagccct acagttgctg caagaaacgg
      121 ggacagacat tcctgggcag tacgaatttt taagtaaggg gaagtgatga aacagaaaat
      181 gacgatacct gttctggctg tggtgctttg tgcgggtacg gtacaggctg acggaaactt
      241 tttgtccctg ccgaaagtga atgacagtgc gtttggctac ggtaaatccg tggacggcgc
      301 agtatcagac aaactgtttt ataccctggg agggggctcg gtgatatcgc agccggcaac
      361 gcgcagcaat atgcagaaac ttggggtgga tctcgggtgg agcagtgatc tgatgtgtgg
      421 caattttgac ctgaaaacaa cagtgggtaa ccagctgaac gggatcactg atggtttcaa
      481 aaacctgatg ggggatgtga ttcagggggc gagcggagcc gtggcgagtc tgccggcaat
      541 ggtgatacag cgggctaatc cggggctgta tgacatgctg accaacggtg tactgcaggc
      601 gaatgtggca ttcgacaaag cgcagctcaa ttgtcagaac atggcgaaac gcatgatgga
      661 gttcacggac agcaataagt ggacgcaggc ggccatgctc gaggagtaca aaaaaattgt
      721 gaatacgggg gacggtgatg cgatcaaagg ggatgaagca ggtcgtcagg ttaccgggga
      781 ggaggggcaa cgctggatcg gagggcagca gcgtggtgga gcgggacagt ctgctatccg
      841 gccgacacat gatctgacag ctgccggttt caatatgatg aacagtctgc cttcgaccag
      901 tacatccagt gtcagtcccg ggaactgtaa cgggagtgcc tgcatgaaat accgtaatgc
      961 agaggaagct gcccgggcgg tggtgaaggt tctgggggat aaatccatgc ggacctgtgg
     1021 tgatgcaaag gcatgtacca gcggggatgc tgatcagcag ccggggacca gtattgccgg
     1081 cacggggttt gcgccgatgc ttgaagaagc gacgcgggtt aacacggaac tgctggcagg
     1141 tatggtcaat ggtcgtgaca agccgacagc cgggaatctg gcaaaactga aagccgggag
     1201 tctgtctgtg acagcgggag tgatccgtgc cctgcagcgt gatccggaca atggtgccct
     1261 gacgtcgcgt ctggcgggag aactggccat ggcggatacc gttgaaacgg cgctgctgat
     1321 gcgcaggatg ctgctcacgg ggatggccga gccgtatgct gcagctcagc ctgctgcact
     1381 ggaagagggg gaacgccgta ttgaggcgct ggatcgggaa attatggcgc tgaaaaacga
     1441 gatggagctg aagcgggatc tggcgcgtaa cagtgttctg accatcattg aacgggataa
     1501 cgagcgtgtg gggaataacc cgatgatcca gcaggctgac aatgctgaca gccgtatcca
     1561 ttctctcgat gccccggata aggagtgaga ggatgagcag aatttatggc cgggtgaaaa
     1621 agaggatgaa ggtattcgcc ctgtatacgc tgttgctgac agggattacc gtgttttccc
     1681 tctggctggc cgataccggt atacgtcacg cagaaggatt tgcggcc
//
LOCUS       DAZGCQ010000280         1722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000280 DAZGCQ010000000
VERSION     DAZGCQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.283"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1071
                     /locus_tag="ACHK07_005289"
     CDS             <1..1071
                     /locus_tag="ACHK07_005289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105459.1"
                     /translation="NGKADAIDRIGTVIYTGNVNMTQGHGITVGSGQKVKIADDDLQK
                     DSKSFLAIKAHEANVGTNVYVISKNGANNVALLEMTEGDNIITTELSLGNLGVSRWVR
                     PMVVDTGAGDDTIVTGRDLGIGYGDLWIEVNTGIGDDILIVGFGRGGYDVVYGKDGSY
                     RIEETAKYQRLDGDIIIPDNDSGYPNNGGFIEKTTVTMGDGNDSLLLLGGNSLAALKS
                     STLDLGAGNDVIDIVANIDNASNIKGGSGFDTLVIHQGNATSNTFSGFEKIELKDGAA
                     LNLLAENLNSASIEGNKLKVTGDGSSRVDLGNNGDNLSDNGYNWIKGSTESEGDITYN
                     IYTHASVPDVQVLIDERITNVV"
     gene            1231..>1722
                     /locus_tag="ACHK07_005290"
     CDS             1231..>1722
                     /locus_tag="ACHK07_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105460.1"
                     /translation="MGVFNYKSYSFAHVAELVSSSYNLAIYSNTGKVLGLGSFLASRL
                     FAKHPDRINYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNSASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGE"
BASE COUNT          503 a          264 c          418 g          537 t
ORIGIN      
        1 aacggcaagg cggatgcaat agatcggatt ggaaccgtta tctatacagg caatgtaaac
       61 atgacgcaag gtcatggaat tacagttggc tcaggtcaaa aggtcaaaat cgctgatgat
      121 gatttacaaa aagattctaa atcattcctg gccattaagg ctcatgaggc aaatgtaggg
      181 acaaatgttt atgtaataag taagaatggt gctaataacg tcgctctact ggaaatgaca
      241 gagggggata atataattac gactgaactg tcactgggga atctgggcgt ttctcgctgg
      301 gtaagaccaa tggttgtcga tactggtgca ggtgacgata ccatcgtgac tggtcgtgat
      361 ttggggattg gatatggtga tctatggatc gaggtaaata caggtatcgg tgatgatatc
      421 ctgatagttg gttttggtcg aggcggttat gatgtcgtat atgggaagga tggttcttac
      481 cgcattgaag aaaccgctaa gtatcaacga ctggatggag atatcataat acctgataat
      541 gattcgggat acccaaataa tggtggtttt atagagaaaa cgactgttac tatgggagat
      601 ggaaatgaca gcctgttatt gttaggaggt aattctcttg ctgcgctcaa atcatcaacc
      661 ttggatttag gtgctggtaa tgatgtaatc gatattgtgg ctaatattga taatgcttca
      721 aatattaaag gtggttctgg atttgatacc ttggttattc atcagggtaa tgccacaagc
      781 aacacattct ctggttttga gaagattgaa ctgaaagatg gagctgcact gaatttgttg
      841 gctgagaatt taaattctgc ctctatagag ggtaataaac tcaaagtcac aggtgatggt
      901 agttctcggg ttgacctcgg taataacggg gataacctca gtgacaatgg gtataactgg
      961 attaaaggaa gcactgagtc tgaaggggat atcacttata acatttatac acatgcttcg
     1021 gttcctgatg tacaggttct gattgatgag agaatcacta atgtagtgta acaatagtat
     1081 cccctcacag tcgttgctgt ggggggatct ttttcgttgt aagttctact ccaggtatgc
     1141 tcttttactg aaaaaattta tctgagaata atgctttttg tttcttcttg gataaatgaa
     1201 ggatatttaa atgaattcag aggagatttt atgggggtgt ttaattataa atcttattct
     1261 tttgcacatg tagcagaact tgtttcttct tcttataatc ttgctatata ttccaacact
     1321 ggaaaggttc ttggattagg aagttttctt gcatccaggc tatttgcaaa gcacccggat
     1381 aggattaatt attctaccga tgttcttaat gaaggctggc gtgatattac agtggaagaa
     1441 ctcaggttat cttcggaata tgtcgattca agaggttttt atcgctttcc cagtccggtt
     1501 acaggaaact ctgcgtcagg atttggtcct cagttgaaag ttgttgggtt gtttgatgct
     1561 caacagaatc tacagaaaat tgcaatatca ttctgtggaa caaatgatat ctgcgatatt
     1621 gttgattata cacagctcaa taatggaaaa gcgctgtctg aagcagaaaa cctgcttcaa
     1681 agtatcagag actttgcagt cagtaatggt ttaagtggtg aa
//
LOCUS       DAZGCQ010000281         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000281 DAZGCQ010000000
VERSION     DAZGCQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.284"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..690
                     /locus_tag="ACHK07_005291"
     CDS             <1..690
                     /locus_tag="ACHK07_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO8105461.1"
                     /translation="GPRCTLTVDTDAVNVAQLRDLQTVTEKANAALDKRITNNTSAIT
                     KNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNALGIREN
                     TQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAG
                     IAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDSESG
                     VGVSAGMALGW"
     gene            complement(1119..1673)
                     /gene="dolP"
                     /locus_tag="ACHK07_005292"
     CDS             complement(1119..1673)
                     /gene="dolP"
                     /locus_tag="ACHK07_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8105462.1"
                     /translation="MVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQVDDEVLE
                     LRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVDGVQEVYN
                     EIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLGLVTEQEG
                     RAAADVASRVSGVKRVTTAFSYIR"
BASE COUNT          462 a          413 c          424 g          377 t
ORIGIN      
        1 ggccctcgct gtacgcttac tgtcgatacg gatgctgtta acgttgcaca gctcagggat
       61 ttgcagactg tgacagaaaa ggcgaatgca gcgcttgata aacgcataac aaacaacacc
      121 agtgcgataa ctaaaaatct gcaggtggtt aacagtaata aagaggaaat cagtaacatt
      181 aataaaacga caaaaattct ggatgaaaga gtgacagcca atacacagac agcagaagaa
      241 cataagctgc ttatttcgga gaacagcaaa caaatcagta ccaacgctct tggtatcaga
      301 gagaatacac aacggatccg ggatgaggga caggctcgaa tccggggaga cagggaaaca
      361 ctgcgtgcag cgaccggtta taccgacatg cgggtaaacg ggctggagcg taatacgaat
      421 aaacgttttg cgcagcttaa agaggatata gacaaaaaca gaaaacgtgc agatgcaggt
      481 attgcaggtg cgatggcaat gacggggata ccgtccgtcc ccgggaaaaa ggtctcattt
      541 ggtatggcca tggccggata ccggagtgaa ggtgctgttg ctgccggctt ccattttaat
      601 acaagtgaaa acagtgctgt taaagtgaat gcagcctggg acagtgaaag tggagtgggc
      661 gtttcagccg ggatggctct gggatggtaa tgcttccggg ccagaagccg ttcgcagata
      721 ttgttatcca ggggacactg caccaccatt caggaagcga ttcagtttat cgcatcggct
      781 caggatgtaa ttaatacgga caatctggct cctgcaccgg agccagacgt acacgtgtca
      841 acgccacgat ctttgacctg tatggataaa agctcagatt atttaccagc agcgacatcc
      901 ggagcgcacc gggattttat ctgtgagtga acatacccgg gcaaaaaaat cccccgaatt
      961 caggggctgt caaagaggaa ttatgtatat tggttattat aatgagcacg cctccggtat
     1021 tccgcgacga atgttcacgt gctggcatga acggcccgga aacgtgctct tctgattttt
     1081 aacgtaaatt tacactaaag gcaactctga tactgccttc accggatata ggagaaggcc
     1141 gtcgtgaccc gcttcacgcc actcacccta ctggcaacat ctgctgccgc tcgcccttcc
     1201 tgctctgtaa ccaggcccag taaaaagact tcgctgtttt ctgtcaccac cttgacactg
     1261 gatgatttga cacgggcact tgtgagtaac tgagaacgaa tttttgtcgt gagccaggca
     1321 tcaccagcca ttatgaccgg actgacgggt gtcccctgcc ggatttcatt atagacctcc
     1381 tgaacaccat cgacaccgag tgctatctga cgggcacggg cagataattc acggtccgga
     1441 gactgaccga tcagcagcac gcgtccctga tatgcaatct cactgatgcg tgcttctttt
     1501 ctgatttgtt catcttttga aagtgcactg ctgaccctca gctccagcac ttcatcatcc
     1561 acctgcgttc cgacactgcg tgggtcggtt gctgcctttg tcccgacagc ggctgtaccc
     1621 accacagttg cagcaacgca tccctgtaac aataaggctg aaatcaggac aatagc
//
LOCUS       DAZGCQ010000282         1647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000282 DAZGCQ010000000
VERSION     DAZGCQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.285"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..1455
                     /gene="gndA"
                     /locus_tag="ACHK07_005293"
     CDS             49..1455
                     /gene="gndA"
                     /locus_tag="ACHK07_005293"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO8105463.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     DNPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGQPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAGNPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLN"
BASE COUNT          428 a          381 c          403 g          435 t
ORIGIN      
        1 tatctagcga tatatcaatt aatgactaac ccctgacagg agtaaacaat gtcaaagcaa
       61 cagattggcg tcgtcggtat ggcagtgatg gggcgcaacc ttgcgctcaa tatcgaaagc
      121 cgtggttata ccgtctctat tttcaaccgt tcccgtgaaa agacggaaga agtgattgcc
      181 gacaatccag gcaagaaact ggttccttac tatacggtga aagagtttgt tgaatctctg
      241 gaaacgcctc gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgctatt
      301 gattctctca agccatacct cgataaaggc gacatcatca ttgatggtgg taacaccttc
      361 ttccaggaca ccatccgtcg taaccgtgag ctttctgcag aaggctttaa cttcatcggt
      421 accggtgttt ccggaggcga agaaggtgcg ctgaaaggtc cttccattat gcctggcggg
      481 cagaaagaag cctatgaact ggttgctccg atcctgacca aaatcgccgc cgttgctgaa
      541 gatggccaac cgtgtgttac ctatattggt gccgatggcg cgggtcacta tgtgaagatg
      601 gttcacaacg gtattgaata cggtgatatg caactgattg ctgaagccta ttctctcctg
      661 aaaggcggcc tgaatctctc taacgaagaa ctggcacaga cctttaccga gtggaataac
      721 ggtgaactga gtagctacct gattgacatc accaaagata tcttcactaa aaaagatgaa
      781 gatggtaact atctggttga tgtgattctg gatgaagcag ctaacaaagg caccggtaaa
      841 tggaccagcc agagtgcgct ggatctcggc gagccgctgt cgttgattac cgagtctgtg
      901 tttgctcgtt atatctcttc tctgaaagag cagcgtgttg ccgcgtctaa agttctgtct
      961 ggtccgcaag cgcagccagc aggcgacaag gctgagttca tcgaaaaagt tcgtcgtgcg
     1021 ctgtatctgg gcaaaatcgt ttcttacgct cagggcttct cgcagctgcg tgctgcgtct
     1081 gaagagtaca actgggatct gaactacggc gaaatcgcga agattttccg tgctggttgc
     1141 atcatccgtg cgcagttcct gcagaaaatc accgatgcat atgccggaaa tccgcagatc
     1201 gctaacctgc tgctggctcc gtacttcaaa caaattgccg atgactacca gcaggcgctg
     1261 cgtgacgtcg ttgcttatgc agtacagaac ggtatcccgg ttccgacctt cgccgctgcg
     1321 gttgcctatt acgatagcta ccgtgccgct gttcttcctg cgaacttgat ccaggcacag
     1381 cgtgactatt tcggtgcaca tacttataag cgcattgata aagaaggtgt gttccatacc
     1441 gaatggctga actaatctta atttagatat ctcgcccgga ttacaccggg cttttcaatg
     1501 cctatagaaa accatcagat aggcttgtag tttagttaag cttacagctt tcggggaatt
     1561 ataaaaattt ttattttatg tcatcaataa aatagaagtg taaatcatga aatatagtgt
     1621 cattaattaa atgcgtactt ctcattt
//
LOCUS       DAZGCQ010000283         1639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000283 DAZGCQ010000000
VERSION     DAZGCQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.286"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(373..1198)
                     /locus_tag="ACHK07_005294"
                     /pseudo
     CDS             complement(373..1198)
                     /locus_tag="ACHK07_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1349..>1639)
                     /locus_tag="ACHK07_005295"
     CDS             complement(1349..>1639)
                     /locus_tag="ACHK07_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8105464.1"
                     /translation="LSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRG
                     WFIARGFRAERIHATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
BASE COUNT          423 a          417 c          345 g          454 t
ORIGIN      
        1 acaagataga aaatcatcac aacaaaggga aaggtctgta gataaagagt gggatggact
       61 tcttacttca tatcacataa ttgatatttt ttatcctgca aactccaacg gcattatgta
      121 tgacttagcc tcaggcaatt atcacaggca aaaaaacaat atattgtaag cttcatagaa
      181 tttggaactt tatataataa ataattctga gttttttatc accattacat taacaaaatt
      241 aatgtattcc agagatttca cattagtctc caatttttca gaagaatcta tttacctggc
      301 aggaaaagtc atctggtgtt tcgccggagt gaggggtaca ggcaatagcg gacataacca
      361 tttgttcttt ttctaagcag ccatctgata atattttatc ctgaaggctg tcggggagat
      421 attacccaga cgagagtgac gacgctgacg attgtagaaa atctcaatgt attcccgtat
      481 tactgagatg gcctcatccc ggcttttaaa acgatagtgg ctcagacttt catttttcag
      541 cgttccccag aagctttcca tcggagcatt gtcgtaacag ttaactttac gcgacattga
      601 tattttcaga ccaaactgct cctgtatggc cctgtaatcg tatgcgcagt actgtgaacc
      661 acgatcggag tggtggatta gcccggtagg tgagcgctgg ttcctgagcg ccataaacag
      721 ggctttaccc gtcaactatt tcgtcatgcg ctctcccatg gcgtaaccga caattttgct
      781 cgtataaaca tctttgacgc cagcgatata cagccatcct tccggggggc aacatacgtt
      841 atatctgcaa ccaagacctg attaggtgct gtaggcgtaa agttctgatt caggagattc
      901 ggcgcaaccg gcagattgtg accagagttc gtgttcgccc tgaacttgcg tttcagctta
      961 cagtgcagcc tcagctcctt acgaagacgt gccagtctgt cacgaccgac gataattccg
     1021 ttatctgcca gctccgtctg gagcagctag gttccatatg tttcgcgtgt gcggatatgt
     1081 gccaccttaa tctccagttt tagccgctca tcactttgtt ttctgtctga gggtcatgct
     1141 gtactcagtc gtaataaccg atcctgtata caccaagtac ccgacacatc gcttcaatac
     1201 ataatcgcat ctgaatcgtt caccgattcg cggcattttt caaggtagtt gtcgccattg
     1261 ttcgactaac tgatcgacat tacagcctag agcctagctg ggggctgtgc acaaaaaagc
     1321 cccggacatt ccggggctac actgcatatt aacgaacgct ctgtccggtt atttcaaccc
     1381 tgcgattggg gcgcagacat tccttcagtg cttcgccctc cacatccggg cagaacacca
     1441 ctggtgcatc atatccacgg cccgttgcat gtatcctttc agccctgaat ccccgggcaa
     1501 taaaccatcc cctgactgat tccgcccgtt tctctgacag gagccggtta tacgcctgcc
     1561 cgccaagatg gtccgtaaat ccttctatct tcaccccaac aacatgaccc gcctgaaact
     1621 tctctgccac acgggaaag
//
LOCUS       DAZGCQ010000284         1635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000284 DAZGCQ010000000
VERSION     DAZGCQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.287"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..557)
                     /locus_tag="ACHK07_005296"
     CDS             complement(<1..557)
                     /locus_tag="ACHK07_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO8105465.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTEQRFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNR"
     gene            815..1315
                     /locus_tag="ACHK07_005297"
     CDS             815..1315
                     /locus_tag="ACHK07_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8105466.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRKLKPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            1317..1610
                     /locus_tag="ACHK07_005298"
     CDS             1317..1610
                     /locus_tag="ACHK07_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO8105467.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLLISQDRLTH"
BASE COUNT          569 a          295 c          251 g          520 t
ORIGIN      
        1 ttcctgtttt tgtttacaag aattttatcg atatgtctca gctgcggatc attactggca
       61 ttaacaatat aaaaagcaat gaaaatgata gcaaaaagaa aaaaaagcat tacatactta
      121 aacagcttac tgctaaagaa acgttgttct gtagagataa tgttattttc aataatgcct
      181 ggcaatgaat actgccaaac ttcatttttt tcttttacag caaatttgag acctaaaact
      241 tctatttgct gattcaatgt taacgttcta tcttcaggtt cttgtgctgt taattctctg
      301 attgttacaa ccagatcagg gtcggtagta attattatct caaaactacc tgtaagctcg
      361 ccataaggaa cgactattgt attttctggt aactcagaaa aagcatcact ttttcctaac
      421 gaaacatcat taccaatgat aacaagtaga cgtccattaa gtatttcata ctcgcatcca
      481 ttcaatggtc cgctcaacaa acgaatggca taagattcca ataagtcttg tgataagaat
      541 ttatcattat tttccatgaa taccacccgc taattccata agctctagtt tagagtagtg
      601 taacaagacg gaaatttcat gaccaaatta tgatgacaca gaataaaaaa attataattt
      661 cgaggactat tttaaatgcc tctatgcata actttattta caatttaagg tataaaccct
      721 attccctcaa tgttaagcaa atataacaat tactcttatt ttaactttta cacgtaatat
      781 ttaccctaaa tgattttaga gtacaggttg gcatatgcaa gtcttttcca gtgatgtcta
      841 cttcactgta ggcactaatg cattattagc atcccaaaaa gaatattaca gcgatttagt
      901 tgcgttggtt gatcttgggc attcgttcgt tgttattgat gagcatcaac atcgaaagct
      961 caaaccaaat acagaacctg taaacatcct tttaagcaac aatttcatca ggatcaataa
     1021 gaatattacg ttatctgatt taacgcattt cctgatttcg aatcttcata cacaaaatgt
     1081 gtattccaca caagaaccac ttacccatga tgagatagat atcctgcggt tatgtgtttc
     1141 ttactcgtta aaacaaatag ccattattaa aggaattgat tataaaacag tatcatatca
     1201 caaaataaga gccctaaata aacttaacat aaaaggtact gttgagttgt ttatagcact
     1261 ctgtgaatgg gataaacatt attttaaact ccaatcttgc gtaagagaaa gttgatatgt
     1321 cattagtagg atataatagt aaaatcataa taaccatcgc gctgatatcg ttgctctctg
     1381 catgcaccag agaaaattca ttaaaggaga atcaaaataa aagaagtggc gaatcccaag
     1441 cgatagtaga tgcaagacag ttctttagcg aacacccgga atatatatct tcagcagaac
     1501 tggagcaaaa gctatatcgc gaatttgcac aggttactac agttccagct tataaagaga
     1561 tgagtatgta tcaattgctt ctcatttcgc aagatcgttt aactcattaa ataatgaatc
     1621 tacacgcttt ttgcg
//
LOCUS       DAZGCQ010000285         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000285 DAZGCQ010000000
VERSION     DAZGCQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.288"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..560)
                     /locus_tag="ACHK07_005299"
     CDS             complement(<1..560)
                     /locus_tag="ACHK07_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8105468.1"
                     /translation="MLMSVQKENNVAGNVVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKVLIDHHIAELDDQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALVSAW"
     gene            complement(581..1078)
                     /gene="tssB"
                     /locus_tag="ACHK07_005300"
     CDS             complement(581..1078)
                     /gene="tssB"
                     /locus_tag="ACHK07_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8105469.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSYG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
BASE COUNT          459 a          423 c          335 g          417 t
ORIGIN      
        1 tcccaggccg aaaccagggc agcaatgggt tcaccacccg gcgtgtcata ttcagccaca
       61 taggtctgca gataaaggcc gctctgaata atttccggcg catcctcaaa atcctggcgc
      121 agatcgtctt tggacagatc cagcagctca attttcacat tacggcggaa gtccgtttta
      181 tccaccagag acttcacgcc ccgccacagg gattccactt tctgaaattc cggatgatga
      241 agcacggcat ccagctgacg actgatctgg tcatcaagct ctgcaatatg atggtcaatc
      301 agcaccttat ccagtttctc caccggtgct cccgctttcg ccaggcattc cataaacacc
      361 tgcatccctg cggtcagacg ttcatctgct gatgcctctg acatggcctg cggatcctgc
      421 cagatatcca gcgcactcag ggctgatacc ggattcagat taattttttc aaacagggaa
      481 gcatatacgc tgtcgccgga atgcgtttca gaaaccacat ttcccgccac attattttct
      541 ttctgtacag acatcagcat gctctctctt aatccgttaa tcacacctgt tccggcacca
      601 gtgcactcag ttcgtcacgt aaggcctgac tcagttcggg atcccgcaga attttttcca
      661 gttctttacg gaaagtggcg ttatccagca gattcgcctt cagatcccgc agcaaacttc
      721 gcatggccag catggcttta agacggggaa tttgtcgggc aacctgctcc ggagtgaaat
      781 cgttcatatt acggaaagtc aggctgatgc tctcttcagt accatcaccg gcaagtgtgt
      841 tctccacatt caggcgtact gacggcgaga tatcagccag aacagcatta aagttatttt
      901 tattaacatc catttttatg cgctctgaca gcgggcgctc ttcctgtccg taactgaaat
      961 caccggcaac cagcagtttg agcggaagct ccactttctt ctgcccacca cctgtatgta
     1021 ggtctaattt cagattcacc cttgccttcg ggatctcatt ctgaaacgaa ctgctcatga
     1081 cgttctcctg tgaaacctgc atcccaggga gcccatcgcc atcgccacag aaggcctccc
     1141 cttgtgcact gatttaattt cacccattat aggacgaaga tctgcaaatt ctcaatatga
     1201 cagacatatc gatcgtttta atgccaataa ccagatcttt attctggtac atatcgcaaa
     1261 atcagcattt atcaaaactt atatccagga gtaatacaaa aatgcagaaa attattccgg
     1321 ttagtgcttt tgatacagat ggttataccg aatgattacc agcactgaca ttcacgagga
     1381 gtgatgattc ggcagcattt aagggcatat caaaaaaaga gaaggacatg aaagaggaga
     1441 gcaataaaag acacggaaaa taaagaagag agagaaaata tttttaaaaa cagaaaatta
     1501 acaacgcaca tcaggaaacg tcgtctcctg attcagtgat accacatgaa aatttagtga
     1561 acggttaccg ttctgaagtc tatgtcagaa gatattccat taccagaaat caatcagcga
     1621 tacagagacg atca
//
LOCUS       DAZGCQ010000286         1611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000286 DAZGCQ010000000
VERSION     DAZGCQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.289"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..388
                     /locus_tag="ACHK07_005301"
     CDS             <1..388
                     /locus_tag="ACHK07_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105470.1"
                     /translation="TICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNI
                     RVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCR
                     IRSNNCYGQISRRDQESALACWGIDR"
     gene            545..727
                     /locus_tag="ACHK07_005302"
     CDS             545..727
                     /locus_tag="ACHK07_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105471.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            742..873
                     /locus_tag="ACHK07_005303"
     CDS             742..873
                     /locus_tag="ACHK07_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105472.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            1096..1281
                     /locus_tag="ACHK07_005304"
     CDS             1096..1281
                     /locus_tag="ACHK07_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8105473.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          396 a          374 c          467 g          374 t
ORIGIN      
        1 gaccatctgc cgtggagcca cccgggtgga tggtaagcct gttattcctg gcatgaagct
       61 gtcgaagggg aaatgcgacc aggttaacgc cattgagcgt gataaggcgc tggcatgggt
      121 ggagaaaaac atcagagtgc cactgactga accccagaaa gcgggtattg cgtcattctg
      181 tccttacaac attggcccgg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc
      241 aggcgatcgc aggggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      301 ctgccgtatc cgctcaaaca actgttatgg tcagatatcc cgtcgtgacc aggagagcgc
      361 gctggcgtgc tggggaatcg acagataagc agaatatttt gctgaaaaat gacgttggcc
      421 aacgcggacg gataacacga aatcctgcga actggcaaaa cctaagtgaa taaaagtaaa
      481 aaccccgttt gttggcagca agcggggttt tgtgtttctg accttggata aggcaaggga
      541 gaacatggaa aagtataaac gaattctgtt gaggttgact atgaaaaacg gccttgaact
      601 gaaagcgcca gtaactgatg acatcagcag agcactggct tttgctatta agtgggtggc
      661 ggtcggtatc gctgtgtctc cgatgctgta tgggctggca aaactggtca ttgcgttgaa
      721 atcgtgaagg gaggattaag catgtcagac aaactcataa tgctggcgaa gatcctctgt
      781 gtaatcgtcg gtatttcatt ttcactaatg ctggttgcta tttttctttt cctgccctgg
      841 atgatgttgt cttcgtcggg gatgctgggg tgagcataaa ccgaatgctt tccgcgttta
      901 ccgttattct gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata
      961 acgccatcac ctacaaagcg cagcgcgata aaaaagccag agagctggaa caggcgaatg
     1021 ccaccattac tgacatgcag gtgcgccagc gtgatgttgc tgcgctcgat gcaaaatact
     1081 cgagggaatt agccgatgcg agagctgaaa atgaaactct gcgcgctgat gttgccgctg
     1141 gtcgtaagcg cctgcggatc aacgccacct gtccaggctc cgtgcgtgaa gcccccacca
     1201 cctccggcgt ggataatgca accggccccc gactggcaga cacagttgaa cgggattatt
     1261 tcaccctcag agagcggttg atgatgatgc agaagcagct ggaaggggcg caggaatata
     1321 tccgcactca gtgcctgaaa taagttttgc tgatgcgccg tatcgtcgcc gtattcccgt
     1381 attaacagag accgcagccc gacagggaga ctcctctgcg agagtgtgcg gggataatca
     1441 aaaacgatac acaccggggt ttaccgcgtt aacagagcgc ggcgttgtcc cctcatagtc
     1501 gcctgtccgg tgcgatggtg gaagaaactg gatttgttgc aactgataac cattatcatt
     1561 ttctcgggtc ctttccggca tatggacccg ttacggggcg gcgacctcgc g
//
LOCUS       DAZGCQ010000287         1601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000287 DAZGCQ010000000
VERSION     DAZGCQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.290"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1077
                     /locus_tag="ACHK07_005305"
     CDS             <1..1077
                     /locus_tag="ACHK07_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO8105474.1"
                     /translation="FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTE
                     VLNNRTTDVINNHAETIGNNQIIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIG
                     LVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKT
                     KKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINW
                     NCAASKSPPKTPDEKQDTPDMREY"
     gene            1144..>1601
                     /locus_tag="ACHK07_005306"
     CDS             1144..>1601
                     /locus_tag="ACHK07_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8105475.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDG"
BASE COUNT          397 a          402 c          495 g          307 t
ORIGIN      
        1 tttgcggtaa taccggcaga cagaacatgg cgaccacagc cgttgctgaa accgctggtg
       61 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgag
      121 catggccgcg tgcgggtgaa atttaactgg gaccgttata acccgtcaaa ccaggacagt
      181 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa ccttgcgata
      241 ccgcgcgtgg gtcaggaggt gattgtggac ttcctcaacg gcgatccgga ccagccgatc
      301 attatggggc gcacctacca ccaggaaaac cgcacacccg gcagcctgcc gggaacaaag
      361 acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      421 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
      481 gtgctgaata accgcaccac ggatgtgata aataaccatg ctgagaccat cggcaacaat
      541 cagataattg cggttaccaa caatcagata cagacggtgg gcgttaacca gatagagacg
      601 gtgggcagta accagatcat taacgtgggt tctgttcagg tggaaacgat tggacttgtt
      661 cgtgcgctga ccgtgggcgt ggcgtatcag acgacggtag gtggcattat gaacacctcg
      721 gtggctctga tgcagtcctc gcagatcggt ttgcataaat cgttgagggt cgggctgggt
      781 tatgacgtca aagtcggaaa taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat
      841 accgggcaga ccgcgattta ctccgccggt gagcatctgg agctctgctg tggtaaggca
      901 aggctggtgc tgacgaagga cggacaaatt tttctcaacg gcacaaaaat tcatttgcag
      961 ggtaaggagc aggttaatgg tgactcactg ttgattaact ggaactgtgc agcctcgaaa
     1021 tccccaccga agacccctga tgaaaagcag gatacgccgg atatgagaga gtactgatcc
     1081 cgtacctgtt cggtattcag taacaaacaa gcattaaaaa taaacagaag aaggaattga
     1141 atcatgagcg gaaaaccagc ggcgcgtcag ggagatatga cccagtatgg cggtcccatt
     1201 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
     1261 ccgggcggga tgacttcggg caacccggta aatccgctgc tgggggcgaa ggtgctgccc
     1321 ggcgagacgg accttgcgct gcccggcccg ctgccgttca ttctctcccg cacctacagc
     1381 agctaccgga cccggacgcc tgcaccggtg ggcgttttcg gccccggctg gaaagcgcct
     1441 tctgatatcc gcttacagct acgtgatgac ggactgatac tcaacgacaa cggcgggcgg
     1501 agcattcact ttgagccgct gctgccgggg gaggcggtgt acagccgcag cgagtcaatg
     1561 tggctggtgc gcggtggtaa ggcaacgcag ccggacggcc a
//
LOCUS       DAZGCQ010000288         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000288 DAZGCQ010000000
VERSION     DAZGCQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.291"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..487
                     /locus_tag="ACHK07_005307"
     CDS             32..487
                     /locus_tag="ACHK07_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO8105476.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            566..799
                     /locus_tag="ACHK07_005308"
     CDS             566..799
                     /locus_tag="ACHK07_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8105477.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            899..>1585
                     /locus_tag="ACHK07_005309"
     CDS             899..>1585
                     /locus_tag="ACHK07_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8105478.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLK"
BASE COUNT          399 a          416 c          416 g          354 t
ORIGIN      
        1 gtcatcgtca tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat
       61 taccgccttc attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta
      121 catcagtata cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt
      181 ccggctggat aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact
      241 caaagacaaa tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cttttggtga
      301 cagtaatgcc acgcgaacca cagacgcatt tctggaaacg gagtgcgtgg aaaatgtcgc
      361 cacaaccgaa atcattaaag caactgaaga aagtaacggc catcgtgtca gtctgccgtt
      421 atcggttttc aatccccagg actaccatcc actgctgatt accgtttccg gaaaaaacgt
      481 taactgatcc ctgaagtcag tttcatgctt cagcctctgt cacaacgcgt ggcagaggct
      541 ttttatttat caggagaaaa cagatatgcc gggttacaca gagtatgtcc tggctgaagg
      601 atctttctca tacgggcagg cggttgctgt cataaccgct taccgcaatg tgtttatcca
      661 ggatgacccg ggaatgcatt tccgtcgggt tatccgcaac gcggaaggtc agcgacgctg
      721 gcgctgccgg aattctgaac ccgatgccgg aaaggtgctt aatacgcggc tcgccagtga
      781 cggtcttctc agacaataaa cgtcattcat ttcacccatc aggccgcgtc ttctccggga
      841 gacgtggctt tttcatttat tacaccagta aatcttaacc accgataagg agcaaattat
      901 gcgactagcc agtcgttttg gttatgcgaa ccagatacgc cgtgaccgtc cgctgacaca
      961 cgaagaactg atgcaccatg tgccggggat tttcggggaa gataaacaca cgtcccgaag
     1021 ccagaactat acgtacatcc ccaccatcac cgtactggaa agcctgcagc gggaaggctt
     1081 tcagccattc ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa
     1141 acacatgctg cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat
     1201 tctgctcaac tctcatgacg gtacctccag ctaccagatg ctgccgggtt acttcaggtt
     1261 cgtctgccag aacggatgcg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg
     1321 gggaaatgtg gtggacagag tcattgaagg ggcttacgag gtggtggggg tttttgaccg
     1381 gattgaggaa aagcgtgatg ccatgcagtc gctgattctg ccgccaccgg cacgccaggc
     1441 gctggcacag gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc
     1501 cgacattctg acgccacgac gccgggagga ttacggtaag gacctgtgga gtgcttatca
     1561 gaccattcag gagaatatgc tgaaa
//
LOCUS       DAZGCQ010000289         1560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000289 DAZGCQ010000000
VERSION     DAZGCQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.292"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..492)
                     /locus_tag="ACHK07_005310"
     CDS             complement(<1..492)
                     /locus_tag="ACHK07_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105479.1"
                     /translation="MGLFNYKDYHQAQITDLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPALGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGE"
     gene            complement(551..>1560)
                     /locus_tag="ACHK07_005311"
     CDS             complement(551..>1560)
                     /locus_tag="ACHK07_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105480.1"
                     /translation="EFYRQALTSNVSGDLYPQPSIFGDKVSSVSKNWSTLLDSNPRSY
                     VTSQRLDSGVNQYNYNGHTGSDVISITDSLGGLDRTQASRFPVGLFTGEGNDLIVTGR
                     DYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVTLYVNEEGQLRATTDSYNGSTSI
                     DYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGNSADAIINTNIDLGAGNDFIYAN
                     GEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSGFEHVDLHSTSHLILNSDDFKSQ
                     DIEGGILKISGSSGASVDVQNFDWENLGSANDGDVKYFTYQSSDISGLTLWIQEGIEV
                     K"
BASE COUNT          482 a          355 c          249 g          474 t
ORIGIN      
        1 ttcaccactt aaaccattac tgactgcaaa gtctctgata ctttgaagca ggttttctgc
       61 ttcagacagc gcttttccat tattgagctg tgtataatca acaatatcgc agatatcatt
      121 tgttccacag aatgatattg caattttctg tagattctgt tgagcatcaa acaacccaac
      181 aactttcaac tgaggaccaa atcctagcgc agggtttcct gtaaccggac tgggaaagcg
      241 ataaaaacct cttgaatcga catattccga agataacctg agttcttcca ctgtaatatc
      301 acgccagcct tcattaagaa catcggtaga ataatcaatc ctatctgggt gctttgcaaa
      361 tagcctggat accagaaaac ttcctaatcc aagaaccttt cctgtattgg catatactgc
      421 aatattataa gacgcagaga tcaggtctgt tatttgcgct tgatgataat ctttataatt
      481 aaagagtccc atgagtattt ccttatttaa agaacagccc catgctgggg ctgtttgttt
      541 agattttctt ttatttaacc tcaataccct cctgtatcca aagagttaaa ccagaaatat
      601 cactggactg ataggtgaaa tatttaacat caccatcatt ggcactacct aaattctccc
      661 agtcgaagtt ttgtacatcc acggatgcac ctgaactacc tgagattttt aatatccccc
      721 cctctatatc ctgagattta aaatcatctg aattcagaat gagatgagat gtagagtgca
      781 aatcaacatg ctcaaaacca gagaacatag cacttgttac tgtagtattg tccaaagata
      841 tcgtgtcaaa gccctctcca cctataatat ttacctgagt accattgtta gttgaaatct
      901 ctccattagc gtaaatgaag tcattacctg cccccaagtc tatattcgta ttaataatag
      961 catccgcact attaccctca taccctaaag ccagaacagt atcatttcct gcccccataa
     1021 caatatcagt tcctgatatt gttccacctg atgatgaaga attgagccca gtatagtcaa
     1081 tggatgtact accattataa ctatcagtag ttgctctcag ttgtccttct tcattcacat
     1141 atagtgttac atcgttattt ccgacaccaa caaccaaggt atcatctcca ttccccatat
     1201 cagtacgatg agagtgatcc gtgtatcctg cactggtatt acgtccataa tctcttcctg
     1261 taacgattaa atcattccct tctcctgtga ataaaccaac cgggaagcga gatgcttgag
     1321 ttctatctaa tcctcctaaa gaatcggtaa ttgagataac atcagaacct gtatgaccat
     1381 tgtaattata ttgattaact cctgaatcta atctctggga tgtaacataa cttctaggat
     1441 tactatcaag taaagtagac cagttcttag aaacagaaga taccttatca ccaaatatgg
     1501 acggttgagg atataagtct cctgatacat tagaggtcaa tgcctgccgg taaaactcca
//
LOCUS       DAZGCQ010000290         1537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000290 DAZGCQ010000000
VERSION     DAZGCQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.293"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHK07_005312"
     CDS             complement(<1..332)
                     /locus_tag="ACHK07_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8105481.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLI"
     gene            complement(332..1495)
                     /gene="emrK"
                     /locus_tag="ACHK07_005313"
     CDS             complement(332..1495)
                     /gene="emrK"
                     /locus_tag="ACHK07_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO8105482.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT          426 a          332 c          308 g          471 t
ORIGIN      
        1 aatatcagca catcaagatt ggtcgataaa ctacacatta atgaagacag cgaaaaaaaa
       61 gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca
      121 gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt
      181 gatgctccta gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac
      241 atttgcataa acgtcgctaa tgacaatgca atagtgacgc accataacgt cccaccggtt
      301 aatggtgccg gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt
      361 tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg gtataagccg
      421 gcatggacgt cacggttgaa gccagctcag gcatctcagc aatgtcttcg ttcttcgtat
      481 caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt tcttttggat
      541 caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt
      601 gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc acccggccat
      661 gaaacacaac attctcacca taaagatcgc tgataatatt gaccgattga ccaatccgta
      721 catccgtgag ttgtgtttct ttaaagttgg cattaaccca catttgacgt gctggtacga
      781 cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactt cgctgggcaa
      841 tatagccggt aaccggactc ttaatatccg tacgtttaag cgccaaccag gcttctttgg
      901 ttgcatccgc agcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag
      961 ctttattcgc tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg
     1021 tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggcacac
     1081 gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac
     1141 tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt gccagattat
     1201 ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac
     1261 cctgtcgaac gtagttcgta tctttatgat taactacagt gacactacct gagacttgtg
     1321 cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta
     1381 attccattga ccaataggca taagcacctg aaaacgcaat aaataaaact atcgccaata
     1441 aagaaaagta ttttcttctg ttagaatgtt ttttatttga attaatctgt tccactttta
     1501 tctctcattt cgcatagatg aaatttatga attgaat
//
LOCUS       DAZGCQ010000291         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000291 DAZGCQ010000000
VERSION     DAZGCQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.294"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1315
                     /locus_tag="ACHK07_005314"
     CDS             38..1315
                     /locus_tag="ACHK07_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8105483.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQ
                     TVTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEE
                     HKLLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERN
                     TNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKISFGMAMAGYRSEGAVAAG
                     FHFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT          483 a          303 c          388 g          352 t
ORIGIN      
        1 tattatgtat actatataaa agagatggtg aattcaaatg cgtaaaatca gtgtcagtta
       61 tatatgcaaa tcattatttc tctactgctc agcttcttta cctgtcttat ctgcaaatgg
      121 tattcctgat aaaatgatcg tggaacttgg tgataacagt gcggcaaaag ctaatttcgc
      181 tgttgctgtt ggtcgatatt cacaggcaaa tgcagaaaaa agtatttctt taggtgcatc
      241 agcaagagct attgaaaata actctaccgc gttaggagca aacgcaacaa cattgaaacc
      301 attctctgtc gctctgggcg caggaagtgt tgctgatcgt ggcgctggta ttgaagcata
      361 tatgccagag acaaagcgct ggaatccagg ggatgtagta aaatccagga tgtctgacaa
      421 gcagcttaac aggcttatgg aaatcgataa gataatatcc gcttataatt cacaagggac
      481 gctgtctgct gaagagacta aacaactgga ggaagtgaaa aaagagcggg ctgatatgtt
      541 tgcgacatat atggcaacgc acgatgctgt atctgtaggt gttggtgcaa cgacaacagg
      601 acgacatgct gttacaagac agataactag cgttgcagca ggttctgtcg atacggatgc
      661 tgttaacgtt gcacagctca gggatttgca gactgtgaca gaaaaggcag atgcagcgct
      721 tgataaacgc ataacaaaca acaccagtgc gataactaaa aatctgcagg tggttaacag
      781 taataaagag gaaatcagta acattaataa aacgacaaaa attctggatg aaagagtgac
      841 agccaataca cagacagcag aagaacataa gctgcttatt tcggagaaca gcaaacaaat
      901 cagtaccaac actcttggta tcagagagaa tacacaacgg atccgggatg aaggacaggc
      961 tcgaatccgg ggagacaggg aaacactgcg tgcagcgacc ggttataccg acatgcgggt
     1021 aaacgggctg gagcgtaata cgaataaacg ttttgcgcag cttaaagagg atatagacaa
     1081 aaacagaaaa cgtgcagatg caggtattgc aggtgcgatg gcaatgacgg ggataccgtc
     1141 cgtacccggg aaaaagatct catttggtat ggccatggcc ggataccgga gtgaaggtgc
     1201 tgttgctgcc ggcttccatt ttaatacaag tgaaaacagt gctgttaaag tgaatgcagc
     1261 ctgggacact gaaaatggag ctggcgtttc agccgggatg gctctgggat ggtaatgctt
     1321 ccgggccaga agccgttcgc agatattgtt atccagggga cactgcacca ccattcagga
     1381 agcgattcag tttatcgcat cggctcagga tgtaattaat acggacaatc tggctcctgc
     1441 accggagcca gacgtacacg tgtcaacgcc acgctctttg acctgtatgg ataaaagctc
     1501 agattattta ccagcagcga catccg
//
LOCUS       DAZGCQ010000292         1524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000292 DAZGCQ010000000
VERSION     DAZGCQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.295"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..596
                     /locus_tag="ACHK07_005315"
     CDS             <1..596
                     /locus_tag="ACHK07_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8105484.1"
                     /translation="PEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWL
                     VCRGDIHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNL
                     YLDNLAENGFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRER
                     YSEKWQRRTHSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            593..1174
                     /locus_tag="ACHK07_005316"
     CDS             593..1174
                     /locus_tag="ACHK07_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8105485.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          354 a          359 c          485 g          326 t
ORIGIN      
        1 caccggaaga ctggctgcag gcacagatgc agggggagat tgtggcgctg gtccacagtc
       61 atcccggtgg actgccctgg ctgagcgagg cggaccggcg gctgcagata aagagtgccc
      121 tgccctggtg gctggtctgc cggggtgaca ttcaccggtt ccgctgtgtg ccgcacctga
      181 ccggacggcg ctttgaacac ggtgtgacgg actgttacac cctgttccgg gatgcttacc
      241 atctggcggg gataacgctg ccggattttg tgcgtgagga tgactggtgg cgcaacggtc
      301 agaacctgta cctggacaac ctggcggaaa acggctttta ccgggtgtct ccgtcccgtg
      361 cacaggcagg cgatattctg ctgtgctgct ttggcgcatc ggtgccgaat catgccgcca
      421 tttactgtgg caacggtgaa ctgcttcacc atatacctga acaactgagt aaacgggaga
      481 ggtattcaga gaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggtccg
      541 catctgcctt cacggggatt tacaacgatt tggtcgccgc atcagcctgt atgtgaacac
      601 ggcagcggag gccatccgtg ccctgtcgct gcaggtgccg ggattccgcg gtcagatgaa
      661 tgaaggctgg tatcagatac gtattgccgg tgaggatacc gcaccggagg cggtgtacgc
      721 ccgtcttcac gaacaactgg gtgagggagc ggtcatccac attgtgccgc gactggccgg
      781 agccggggga aatggtgttt ttcaggtggt gctgggggtt gcagcgattg tcgggtcatt
      841 tttcactggt ggtgcaacac tggcggcatg gggcgcagcc ctgagtgccg gagggctgac
      901 tgccaccacg atgctgttct cactgggtgc cagcatgata ctgggtggtg tggctcagat
      961 gctggccccg aaggcaaaga cgccggagta taaaagtacg gataacggta aacagaacac
     1021 gtatttttcg tcactggaca acatgattgc ccagggtaac ccgatgccgg tgccttacgg
     1081 tgaaatgctg gttggttcac gacggatatc ccaggatatc agcacccgtg atgagggcgg
     1141 agacgggaaa gtggtggtta tcgggcgggg ataaaaataa aaaaaatccc gcagagtcag
     1201 cggagctgcg ggaaataacg atgaagatta acgttatgga gttatttttt tgggcagaaa
     1261 aacattaacg cagcgaaatt ataagcgcca catggatttt gcgaaaatgt gaagaaattc
     1321 agaaattttt tgctgcagag gtgcagggag cttgccgggt atctgtggca gaagacagaa
     1381 aacatcatgc cggggttaag ggataaaaaa atcccgcaga gtccggagac tgcgggacag
     1441 aaccgatgaa gattaacgtt caaggagttt tgtttttatt gacccgaaaa aactgtaacg
     1501 ccccggaatg atatctgcca cggg
//
LOCUS       DAZGCQ010000293         1520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000293 DAZGCQ010000000
VERSION     DAZGCQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.296"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            502..>1520
                     /locus_tag="ACHK07_005317"
     CDS             502..>1520
                     /locus_tag="ACHK07_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105486.1"
                     /translation="MNLRKKHICIAISLVLNPASISQASAQADTNKLTVEVDETISSA
                     GDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEGLYT
                     GESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENNYLA
                     SEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGFNAL
                     ANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGADSRD
                     KQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEKSTD
                     AINGS"
BASE COUNT          444 a          274 c          383 g          419 t
ORIGIN      
        1 tcacactcta tctttacaca attcatcacc attacacctc catagcctgt tgatttaaaa
       61 gatttaattt cctgtgaaac aatcaggcct gtatgaacct gattatacca cataatcttt
      121 taacttattt aaatttaact ctcaggcttt tctgtcagta aaatcagaaa gaattgcagg
      181 atttcgtttt tttgctgcga agtgaaatgt atctcatttt tactgccttc gccagtgcgt
      241 caggcaatgg caggtagtgt acctgcattc tgctgtaacg cgtaatgtcg ggtattttac
      301 ttaatatgtt ttatgtttgc attattataa atcactttat tgccttttgt ctgcttttta
      361 atggttcccg tttttttact tctggcttta tataaatgga tgctgaagaa atgaaggcag
      421 tccgccttac ataatattca tgcatgattt ttatgtcatt ccgactgagt ggaagaaaat
      481 aaaggagaaa agaggatata tatgaacctc agaaaaaaac atatctgtat agccatatca
      541 cttgttctta atccggcatc catatcgcag gccagtgctc aggcagatac aaataagtta
      601 actgttgaag ttgatgaaac catcagttcg gcaggcgatg actggaagag tattgaaata
      661 ggtacaacga aatttacagc aaaagatgaa tcaaagggaa ttaatttcga ccataaatca
      721 ccggtggctg aaggacgtaa ctctatcgcg attggtacca gtgcagaggc tggaggtgac
      781 attagcacga aggaaggttt gtacacaggc gagtctattg cgataggagc gaaaacgaaa
      841 gcaactaaag aacagtcaat tgcaataggt ggcgatacca aagctaccgg atggggggct
      901 attgttattg gtggggatga tttaactcct ttgcatggta agacttatgg tcgggaaaaa
      961 atagagaata actatctggc atcggaagcg aagggggatg gcagtattgc gctgggcagc
     1021 agaacttttg ctaaaggcga attgtcagta tcgcttggtt cggtgagttc tgctgagggg
     1081 atggcatcga atgcaattgg agccacggcg gaagctaaag gggatatggc gactgcggtt
     1141 ggttttaatg cgctggctaa tgccggtaat gctctggccg ttggtagtca gtcctctgca
     1201 aataaaaacg atgctcttgc tgttggttca gaggcaaatg ccgctcagaa tgaaacggtt
     1261 gccctggggc atggagcaac cgttactgtc gcaaagggga acgtgtccat cggcgctgac
     1321 tccagggata agcaggcaca aaaagtaaca gaggctaagg tcagaactaa aagaggcgcg
     1381 gaagtcacat atagtggctt tgccggaaat aacgcagcat cagtagtatc tgttggtgat
     1441 gcgggtaaag agcgtcagat tgttaacgtg ggggcgggtg ctgtatctga gaaaagtaca
     1501 gatgccatta acggtagtca
//
LOCUS       DAZGCQ010000294         1511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000294 DAZGCQ010000000
VERSION     DAZGCQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.297"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..530
                     /locus_tag="ACHK07_005318"
     CDS             <1..530
                     /locus_tag="ACHK07_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8105487.1"
                     /translation="INENNENIDFYIKASSTYKGHIENNSDLVKKVLFSYPLILCCSP
                     DYINKYGIPSNPFSLKHHNCLYASSLVGGNCWHFYIRDEITTLEIAKTVNIENSEFLK
                     AVALMGGGIAYLPYPLVQSEILSGKLVSIMNDYIKTRFEVSLWFRKPLSLRCVTFRNF
                     LVERASQLKDTVQFR"
     gene            704..1511
                     /locus_tag="ACHK07_005319"
                     /pseudo
     CDS             704..1511
                     /locus_tag="ACHK07_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          470 a          313 c          342 g          386 t
ORIGIN      
        1 ttattaatga aaacaatgaa aatattgatt tttatataaa agcatcgtcc acatataaag
       61 gacatataga aaataatagc gacttagtaa aaaaagtgtt gttcagctat ccattgatac
      121 tatgctgttc tcctgactat ataaataaat atggaatacc ctccaatcca ttcagtttaa
      181 agcatcataa ctgtttatac gcatcttctc ttgttggagg gaattgttgg catttctata
      241 ttcgtgatga aataacaaca ttggaaatag ctaaaacagt aaacattgaa aatagtgagt
      301 ttttaaaagc agttgcacta atgggtgggg ggatagcata cttaccatat cctcttgttc
      361 aatcagaaat actgtcagga aaactagtat caataatgaa tgattatatt aaaactcgtt
      421 ttgaagtaag tctgtggttt aggaagcccc tatccttgcg ttgtgttaca tttaggaatt
      481 tcctagtaga aagagcctca caactcaagg atactgtaca attcagataa atcacttaac
      541 ttgatgcatc aatcacccag cgaccagtta tgctcatcat aatcattatt caggtgtgag
      601 cataatgagc ataacccagc gatccagaca ggttccctga atctccagac aaccaatatc
      661 aattaaataa gtgatagtct aaaaactagt cattagagaa cagatgattg atgtcttagg
      721 gccggagaaa cgcggacggc gtaccacaca ggaaaagatc gctattgttc agcagaactt
      781 tgaaccggga ataactgtct ccctcgttgc acggcaacac ggtgtgacag ccagttattt
      841 ctctggcgta agcaatacca ggaaggaaat ctcactgctg tggctgctgg agaacaggtt
      901 gttctggcct ctgagcttgc tgccgccatg aagcacatta aagaactcca gcgcctgccc
      961 ggcaagaaaa cgatggaaaa ttaactcatt agagaagccg ttgaatattg gcgtgcaaaa
     1021 aagtggatag cgcacgccca atattgcccg ggaatgggga gtaagtttcg tcagccgtta
     1081 tctccgtgtg ttgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggaagga
     1141 tggccgccgc atgatacgga tgtttatctc cgtaataaca tggtgtacac catgttatcg
     1201 gagagctgcc cacgtatggt tatcgtcggc tgtgaaacag tacaggacgt catgctggga
     1261 gcggtagaac accgcttcgg cagcgacctt cctgcgtctc cagtggagtg cctgacggat
     1321 aatagttcat gctatcaggc taatgaaaca cgccagttcg cccggatgtt ggaacttgaa
     1381 ccgaagaaca cggcggtgcg gtatccggag agtaacggca tagcagagag cttcgtgaaa
     1441 acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
     1501 aaccttgcag a
//
LOCUS       DAZGCQ010000295         1508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000295 DAZGCQ010000000
VERSION     DAZGCQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.298"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..1334
                     /locus_tag="ACHK07_005320"
     CDS             171..1334
                     /locus_tag="ACHK07_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO8105488.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNIFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1339..>1508
                     /locus_tag="ACHK07_005321"
     CDS             1339..>1508
                     /locus_tag="ACHK07_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFO8105489.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRR"
BASE COUNT          379 a          352 c          405 g          372 t
ORIGIN      
        1 ctcaatattt tacccggttg catcatgatg cctgattata tttcccgcgt gtcccggagg
       61 ttcgggtgaa gagaatatta tctttctgtt ttcttttctc tgtccacaac gtgttcgctt
      121 ctccactgcc ggccctcctg agggatgctc tcattcatga tccatcagtg atggaggcaa
      181 gggcagaagc cacctctgcc cgttcccgtg tggagcaggc tcgttcagca cactggccgg
      241 tcgtcacagc aacaggcagt aaactccttt cccagagtca ccgctacgac tatgactacg
      301 actctgaaga tattttgccc ggtatccgcg gtgaaatgaa tatattcgct tcgggagcta
      361 ttgaggcgga tgtcagccgg agtgagtcgg aagccgagta ttaccgttat aaagttgatg
      421 aggctggcga ggaaacaatt cgctcctttg tttcattata tcttgatgcg ctaagggaaa
      481 agcaatccat tgcggtactt gaacagagtc tctcccggca taacgcaatt cttaatgatc
      541 ttaacaccat cagtattcac gataccgggc gggaatctga atttgttcag gccgaagccc
      601 gcaggctgat ggtccggcag cagataaata cccgcacccg agtgctcaaa accacgctgg
      661 gaaaactttc gacctggacc acaaatccgg taaccgaagc cgatcttgaa aatccttttt
      721 cccggatgac agagtccaga ttgttaactg attttacgca gacgcctcag aaagggaatc
      781 cgtcgtggct tgccagtcag gctgatgtag agagtaaaaa agcggcgctg aaagcgcagg
      841 agcttgcccg gtatccccgg gtggatttaa cgggatcagt gacccgggat gacaggcaga
      901 taggtgtcag tctgtcctgg gacctgttca accgtaatgc cagttatggt gtcagtgaaa
      961 aggccgcgca gatagctgca gctaccggac gactggactc tgttgcccga atgattgatg
     1021 aaaccggacg attatcgctg ataacggtca gacaaagtcg gatggaaatg gaaacgctca
     1081 gacgtcagga gcaggcctct gccagagttg tggactttta tcgtcttcag ttccgggtgg
     1141 caagaaaaac actgattgaa ttactgaatg cagaaaacga actgtacagc gtcgggcttt
     1201 cccgggttca gacagaggat cagatgctcc acggaatgct ggattatctg tattcccagg
     1261 gaatgctcct gaaatggagc ggagtgaatc tttctggtgc agaagaaaaa gtaacagggc
     1321 agaaggacag ataaataagt gaagagtatt tttgaaggat tatcacaggt aacggcatta
     1381 ctgggtacac ctgtatctgc tgagacgctg gctgccggta cagtccggac agatgtctcc
     1441 gggattgatt ttcgttctgt tggcgagttt cttcgcagtg aagggtttga taatcatctt
     1501 tcccggcg
//
LOCUS       DAZGCQ010000296         1505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000296 DAZGCQ010000000
VERSION     DAZGCQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.299"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            362..880
                     /locus_tag="ACHK07_005322"
     CDS             362..880
                     /locus_tag="ACHK07_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8105490.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1090..>1505
                     /locus_tag="ACHK07_005323"
     CDS             1090..>1505
                     /locus_tag="ACHK07_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8105491.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEP"
BASE COUNT          385 a          357 c          379 g          384 t
ORIGIN      
        1 tgttttgtga ttgattaagt gggtattttt aaggaacatc tcaaaaatta tcaaaatgat
       61 aattcagaaa ggataaatac aatatttatt catgattcag ctttgaccat tttatggagg
      121 caatacggac tttgaacagt tgggtatggt tatccagata tatattatta agttattgct
      181 gaataagaga tttaatgatt ttaaaaatag tttttacgac aaccgtaaat tctagctcgg
      241 tttgtatgta aaaaatactg aaaaatgatg cggttccgtg aagtgagctg catctgcctg
      301 tctgggatta acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca
      361 aatgccaaca ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg
      421 tgcatttacc gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct
      481 ggtgcaggaa ttcctgcata acgttactgt cccgaccgat cctcagtccg gtcagcctgc
      541 gggtcagcgt gctcacaagc ctttcatttt taccgtggcg ctgaacaaag cggttccgct
      601 gatgtacaac gcactggcct ccggcgaaat gctgccaacc actgaacttc actggtggcg
      661 tacctctgtg gaaggcaaac aggagcatta tttcactacc cgcctgactg attcgacgat
      721 tgtcgatatg aaactgcata tgccgcattg ccaggacccg gcgaaacgcg agttcaccca
      781 gttgctcgaa gtttcactgg cctaccgcaa aattgaatgg gaacacgtca aatctggcac
      841 ctccggtgcc gacgactggc gcgcaccgct ggaagcataa gttaagccaa cagcatccgg
      901 ctatcaggcc ggatgctgtt tttgtattac gccgtagaaa gaaaggttgt cagtaagggg
      961 ttacgttcac gggagatggc ggctgccgga aacttcggcg gtagcgtgcc tgaacgcaag
     1021 cgtaacactg tactgtcaca gccgctttaa cagcgaactg tctgtttttc cttaaccccg
     1081 gaggttgcta tgtcaaccgg attacgtttc acgctggaag tggacggcct gccaccggat
     1141 gcttttgcgg tggtctcctt tcatctgaac cagtcactct cttcgctttt ttccctcgct
     1201 ctctctctgg tcagccagca gtttctctcc cttgaattcc agcagatcct cgacaaaatg
     1261 gcctacctga cgatatggca gggcgatgac gtacagcgcc gggtgaaagg tgtggtgacc
     1321 tggtttgaac tgggggagaa cgacaaaaac cagaagctgt acagcatgaa ggtatgcccg
     1381 ccgctgtggc gcacagggct gcgccagaac ttccgtatct tccagaatga ggacatcgaa
     1441 agcatcctcg gcacgatatt gcaggaaaac ggggtgaccg agtggagccc gctgttcagc
     1501 gagcc
//
LOCUS       DAZGCQ010000297         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000297 DAZGCQ010000000
VERSION     DAZGCQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.300"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..729
                     /locus_tag="ACHK07_005324"
     CDS             <1..729
                     /locus_tag="ACHK07_005324"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8105492.1"
                     /translation="ETFSIIMPAHNAEATIEYSINSVLNQTDQDFQLIIVNDASTDNT
                     QKIIDRYNDERIIKLVNKVNLGVAETRNRALNIATGRYIAFLDSDDVWRRNKLERQRR
                     EFNNGHLLVCSAYCIMDNDGTVNDKKIFFKENIEYKDMLKSNFIANLTGCYDSQMLGK
                     FYQKKIGHEDYVMWLDILSVAKVAYCIPECLAVYRIANHSVSSNKIRAMHWQFNIYRQ
                     YLKMNIAKAVYYFIHYAINALKKR"
     gene            762..>1488
                     /gene="wzy"
                     /locus_tag="ACHK07_005325"
     CDS             762..>1488
                     /gene="wzy"
                     /locus_tag="ACHK07_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061350267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O27 family O-antigen polymerase"
                     /protein_id="HFO8105493.1"
                     /translation="MKINKNSVFISCIFILNPLASVLCALRSINKKNLGFVIVLISVL
                     TFFITLYTPPYQDLYRRYISTYYIYNSQTTLWEALENKVDFLFYLCSWLFFKLDFPFY
                     LIPALFSSISCYCILSAANDFWRYDKKNVSRYILLIAFLCIFSIIDVIMIASTLRFGF
                     AVALFIKGISTYYVVGKKKRAYAFFLLATSCHVSMLLPVCVIFVNKFIKISWLNCFIL
                     SILMYISSIYLVPFILNIVNLGHL"
BASE COUNT          496 a          185 c          259 g          548 t
ORIGIN      
        1 gaaacattct caattataat gcctgcacac aatgcagaag cgactataga atatagtata
       61 aattcagttc tcaaccaaac tgatcaagat tttcaattaa ttatagttaa tgatgcatcg
      121 accgacaaca ctcaaaaaat aattgatagg tataatgatg agagaattat aaaattagtc
      181 aataaggtga atttgggagt ggcagaaaca cggaatcgtg cactaaatat tgctaccgga
      241 cggtatattg catttttgga tagtgatgat gtctggagaa gaaataagtt agaacgtcag
      301 agaagagaat ttaataacgg tcacttatta gtttgctctg cttattgcat tatggataat
      361 gatggaactg taaatgacaa aaaaatattt tttaaagaaa atatagaata taaggatatg
      421 ttgaaatcaa atttcattgc aaatttgact ggatgttatg acagtcagat gctaggtaag
      481 ttttatcaaa aaaaaatagg tcatgaagat tatgttatgt ggttggatat cctcagtgtg
      541 gctaaagtag catattgcat accagaatgt ttggctgtat accgtatagc gaatcattcg
      601 gtttcatcaa ataaaattcg agcaatgcat tggcaattta atatttatag acagtattta
      661 aaaatgaata ttgcgaaagc agtttattac tttattcatt atgcaattaa tgcgttgaaa
      721 aaaaggtagt tatttatgta tgggcggatg ttaatcgaat tatgaagata aacaaaaata
      781 gcgttttcat tagttgtata ttcatattaa atccattggc ttctgtttta tgtgcattaa
      841 gatcaataaa caaaaaaaat ttaggctttg ttattgtatt aatttcagtt ttgactttct
      901 ttataacttt gtatacacca ccttatcagg atttgtatag gcgatatata tccacatatt
      961 acatatataa ttcacaaaca accctatggg aagctctgga gaataaagtt gattttttat
     1021 tctatttatg ttcatggttg ttttttaagc ttgatttccc tttttacctg attccggcat
     1081 tatttagttc aatcagctgc tattgtatcc tttctgcagc gaatgatttt tggcggtatg
     1141 ataaaaaaaa tgttagtcgt tacatcctgt taattgcctt tctatgcatt ttttcaatta
     1201 tagatgttat tatgatagca tctacattga gatttggttt tgctgttgct ctgttcataa
     1261 aaggcattag cacttattat gtcgtcggta aaaagaaaag agcatatgct ttttttctct
     1321 tagcaacttc ttgtcatgta tcgatgttgt tgcctgtgtg tgtaatattt gtcaataaat
     1381 ttataaagat atcgtggtta aattgtttta ttttaagtat tctaatgtac ataagtagca
     1441 tttatttagt tccatttata ttgaatattg taaatttggg acatttaa
//
LOCUS       DAZGCQ010000298         1483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000298 DAZGCQ010000000
VERSION     DAZGCQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.301"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1302
                     /locus_tag="ACHK07_005326"
     CDS             <1..1302
                     /locus_tag="ACHK07_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO8105494.1"
                     /translation="RLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHR
                     ISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVF
                     NGFELISQQCSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSL
                     WGLALSLPADTDYPDPRGELDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLV
                     SDPLGLLGGKQTYRYVPNPLGYIDPLGLVKTSVPAEKISLSDKARDLFRQGKVREALD
                     VHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKK
                     TIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            1304..>1483
                     /locus_tag="ACHK07_005327"
     CDS             1304..>1483
                     /locus_tag="ACHK07_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105495.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVS"
BASE COUNT          419 a          322 c          445 g          297 t
ORIGIN      
        1 cggcttgacc ggaacggcca ggtcacggcg gtgacagcct caggcaccgg gctgggctat
       61 ggcgaaggcg atgagtccta tggctatgac agttgtggct acctgaaggc gcagtctgcg
      121 ggcgggcacc ggataagtga agagactgac cagtatgccg gaggccaccg gctgaaacag
      181 gccggaaaca cgcagtatga ctatgacgcc gcaggccgga tggtcagccg caccagacac
      241 cgtgacggct atcgcccgga aacagagcgg ttccggtggg acagccggga ccagctgacc
      301 gggtattgca gcgcacaggg tgagcagtgg gaataccgcc acgacgccag cggcagacgg
      361 acggaaaaac gctgtgaccg gaagaaaatc cgttttacgt acctgtggga cggcgacagt
      421 attgcggaaa tccgggaata ccgcgatgat gaactgtaca gcgtacggca cctggtgttt
      481 aacggctttg agctgataag ccagcagtgc agccgggtac ggcagccgca cccgtccgtg
      541 gccccgcagt gggtgacacg aacgaatcat gcggtaagcg acctgacggg ccgcccgctg
      601 atgctcttta acagtgaagg caagacggtc tggcgaccgg gacagaccag cctgtggggg
      661 ctggcactca gcctgcccgc agacacagac tacccggacc cgcgcgggga gctggacgcg
      721 gaagccgacc ccggcctgct gtatgcagga cagtggcagg atgcagaatc ggggttgtgc
      781 tataaccggt tccgatatta cgagccggaa accgggatgt acctggtgag tgatccactg
      841 gggttgctgg ggggtaagca gacttaccgg tatgtgccga atccactggg gtatattgat
      901 ccgctggggc tggtaaaaac ttctgttccc gcagaaaaaa taagcctttc ggataaagca
      961 agagatcttt ttagacaagg aaaagtaaga gaagcattag atgttcatta tgaggatctt
     1021 gtacgaagaa agcttggtgg tatatctcaa gaaattgccg ggcgtgaata tgatgttgtt
     1081 actgacaaga tcatagcgca ggtcaagagg acttatagct ctatagataa tccaaaaaac
     1141 ttcttaagta agtctactcg gacacaaatt aagaagacaa ttgagctggc tgaagaacag
     1201 gggaaagagg ctcagttttg gtttaaatat ggagtaagcc ctaaagtaag agaatatata
     1261 gaaagcaaag gtgggaaagt tatattgggt atggggaatt aacatgtcaa gatatatact
     1321 tattagaggt tggtttgagt gcgactttga acaagtaaaa aaaataaaag aagctaatga
     1381 agaatttttc aaacattctg aaaaatattt attaccagaa aacacgaaac gactttattt
     1441 aaatgggtgg tgttacccag aatatcctat caactgggtt agc
//
LOCUS       DAZGCQ010000299         1473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000299 DAZGCQ010000000
VERSION     DAZGCQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.302"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..214
                     /locus_tag="ACHK07_005328"
     CDS             <1..214
                     /locus_tag="ACHK07_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105496.1"
                     /translation="NPIGINNFSQSSNIANVAGDEVVSLDKHINTSATDTDQIQAFIV
                     STWMASFQNDMYSEDNPISPYYKIEW"
     gene            298..801
                     /locus_tag="ACHK07_005329"
     CDS             298..801
                     /locus_tag="ACHK07_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO8105497.1"
                     /translation="MRSPKVKFLTIFTFCILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIRSLDYGLMQINQMHIPMLKKRGIIQDEQDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNKKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(819..1451)
                     /locus_tag="ACHK07_005330"
     CDS             complement(819..1451)
                     /locus_tag="ACHK07_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8105498.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          501 a          276 c          233 g          463 t
ORIGIN      
        1 taatccaatc ggtataaata atttctctca aagttcaaat atagcgaatg tagcgggtga
       61 tgaagtcgtt tcattagata aacacatcaa caccagtgct actgatacag atcaaataca
      121 agcatttata gtttcaacat ggatggcgtc ttttcaaaat gatatgtatt cggaagataa
      181 tcctatctca ccttactata aaattgaatg gtagacaatt gaccaaataa atcattagcg
      241 agatactatt ataatagtat ctcgttcctt tcattcaatc ctcacacata aaaatatatg
      301 cgctcaccaa aagttaaatt cttaaccatc ttcacgtttt gtatattaat tacaaaaatg
      361 tctttcgcca gcagcgcttg ctttaatgaa gcaggaacaa tgttcagaat cgaaccaaat
      421 ttgattaaag ctatcgctct tgttgaaagc aatctaaaaa aggatagcat aggcaagaac
      481 agagataaga ataacaatat aaggagttta gattatgggt taatgcagat aaaccagatg
      541 cacattccca tgttaaaaaa acgtggaata atacaagatg aacaagattt gttagataac
      601 ccctgtctga acattaaaat aggaaccgag attttatata accatttttc acgttgtgga
      661 gtaacgtggc aatgcctggg gacttataat gcgggttttg caatggataa caaaaaaaag
      721 agactgcaat atgccaaaaa aatatacatt gtatatacca ggcttaatga actagacaat
      781 cgtaaggctt tagcaaaatg agcctaaagc ctcttttttt atatagtgca aacacccata
      841 cgtaaagctg ttttgatcaa ctcgatacca ctgtgaacat ctaatttctt catgatattg
      901 agtctatgag tttcgactgt tttcctgctt aactgcaaga aattagcgat ttctttgttt
      961 gttttaccat cagctatgag ttgtaatatt tctgactcac gatttgtaag ttgattatca
     1021 gaggaatacc tggaagatat tttttcacaa tccatatgta cactatcaaa ataagtgtag
     1081 ccattactaa tcgaatcaat agcgcgattt agtttcgccg ggtgactggt tttccatacg
     1141 catcctttcg cacctgcttc aagcaatttt attgaatcaa tatacggcat ctttgctaca
     1201 aatgtaataa tatgagcatc tattttatgc gatttaaccc attttacgag ttcggacccc
     1261 atcattccac ctgcaaattc tcctaaaata aatatttggg ctgcagattt gttacactcc
     1321 gctatagctt tcttcagatc tttatagccc ccaacaacct catataaatc aggataatgt
     1381 ccgaggaatc caattatccc atcaatcata aacggctgct gatctgaaac tacaatttta
     1441 atttttccca tatattcatc ctgagtgtaa atg
//
LOCUS       DAZGCQ010000300         1466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000300 DAZGCQ010000000
VERSION     DAZGCQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.303"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..342)
                     /locus_tag="ACHK07_005331"
                     /pseudo
     CDS             complement(1..342)
                     /locus_tag="ACHK07_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            complement(349..1466)
                     /locus_tag="ACHK07_005332"
                     /pseudo
     CDS             complement(349..1466)
                     /locus_tag="ACHK07_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT          354 a          391 c          297 g          424 t
ORIGIN      
        1 tgtagcccct tccgcactga taagcgccag cggcaggccg cgatggtcgc agtggtacag
       61 gtggattttt cgcgccggcg tgtacaccgg gtccatctgg ttttgcatct gctccaccgt
      121 caggccgcac gatgccagcc agcggcgact ttcctcactc acccggtcag ccaggatttc
      181 actttccagc cggtcgagca tctgcaccag caccggcggg aacaccacac tgccaccgtc
      241 ttcgccgccg gactgctgaa gcgcatccgc caggctgcgg cgctgcgttt tcgccagctc
      301 accggtggcg gtttcaaccc tgatgagtgg cgtgaagcca gggcaagatt acgaaagccc
      361 gctccccgca aggactgacg ccagatagtt tctgtccatg gctgcttttc gcatcttacg
      421 tcttaaccct gccttgaata ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat
      481 ccctgaaaat aattctgctg catttcctct tcttattttg cagtcgtctt cattcattac
      541 cacgtccaga cgccagtgca gcttattctc cacgtgccag tgatttcgga tcgctgtggc
      601 gaatttctca gcggttaaat cagcagaact gatataatat ctgaccgtca tttctggctc
      661 tttcttttgc tctgctatta tggaccgaaa ggagactgcc acgcataatt tcttcagccc
      721 tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaacaa tatgaagacg
      781 gatttcttct ctgccgtgac tcttttcact aattgcgtaa ctgtcatgct ctggattatt
      841 taattctttc agcggaaatt tttcctcaaa ggctttatta agccgcccct ggttaccttt
      901 taccgcgaat aaataatcac cgccctgttt ttgtatcttc tctgcgatat ctttctggca
      961 acccatcgca tcagttgtga taattttccc tttaatatcc agcatgttaa gaagttcagg
     1021 gatagctgta atctcattag atttctcatc cgtcttgatc tgtccgatga ccagactgtg
     1081 cattgttgag aacgcactaa tgacatgaat cgctcccctg cggcgactct tgtcataaga
     1141 atgccggagc gtttttccat caattgcaat gacgtcttta tcatctgaag aatgacaatc
     1201 acgcatccag ttaataaagc actcatgaaa ttttgtaggg ctgatacagg atacaactct
     1261 ggcaatggta tcgtgaacag gaataccatt ttcaaaatca ccatattgct tcaaaaaatc
     1321 gagatgtgtt tccccaaaat cctctatatc ttcccaacct tctgcacccg aaataacggc
     1381 acaaatagtc aacagtagaa tatctgataa tttatgttcc actttctagg cttgtctgta
     1441 atcggggata atagaaatat gttcca
//
LOCUS       DAZGCQ010000301         1465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000301 DAZGCQ010000000
VERSION     DAZGCQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.304"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            206..337
                     /locus_tag="ACHK07_005333"
     CDS             206..337
                     /locus_tag="ACHK07_005333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105499.1"
                     /translation="MDSENKIQVHKSIDYCIGVVGINNDDVKVKISIQDGNDSKNSE"
     gene            556..801
                     /locus_tag="ACHK07_005334"
     CDS             556..801
                     /locus_tag="ACHK07_005334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105500.1"
                     /translation="MEEMQDKMAWMQNVITEKLGPSLWHMHPIAFPGALIKKNTGWAH
                     SKFANLLGSVELENDYSAYNVTKTGKSVPHYNINLTK"
     gene            972..>1465
                     /locus_tag="ACHK07_005335"
     CDS             972..>1465
                     /locus_tag="ACHK07_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105501.1"
                     /translation="MGCNKKLNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGSPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDNP"
BASE COUNT          495 a          220 c          299 g          451 t
ORIGIN      
        1 cgatttttta cagttcattc aataaaaatg gaacacaatg tgaaacctct tttaatgata
       61 aagttgtttt gaagcatagg tgtgagtatg aatatccttc ctgtttgata tcttattctc
      121 tccttgctta ctcttggaca gaagtttgaa tattccattc cccatgggaa atacttgcga
      181 ggttggagca aaaagaatta tctctatgga ttccgaaaat aaaattcagg tgcataagtc
      241 aatagattac tgtattggag ttgttgggat taacaatgat gatgttaaag taaaaattag
      301 cattcaagat ggtaatgact ctaaaaattc tgagtgaaca acaaattagc gaagttaaaa
      361 tagttgcgtt tcttatctgg tcggtttgat atttttgtta gtatttccat acttgtaaag
      421 ttaatcaaat atcatcaatt gatttagtgt aatttcaaaa tattaatata tcaaaatacg
      481 atgagttaaa cgactttata acgaattcaa aactaatgaa aaatgcttaa atgcactgaa
      541 aaaagacgta ccggaatgga agaaatgcag gataagatgg cctggatgca gaatgtgatc
      601 acggagaaac tgggcccttc gttgtggcat atgcatccga ttgcctttcc tggagcatta
      661 ataaagaaaa atactgggtg ggcgcatagc aagtttgcaa accttttggg aagtgtcgag
      721 ttagaaaatg attattcggc atataatgtt actaagacag gtaaatctgt cccgcactat
      781 aatattaatc tcacaaaatg acattatctg cggtaattga tgccccaaga gaacgaaaaa
      841 tggtaaaaag aaatacaggt tagtacctag ggtggcagaa gttgaggggg agtcgtacta
      901 ttctggtgat ggattgaata aagctcacct attaccagat acgatgatta aagctcttga
      961 ggattctaaa aatgggatgc aataaaaaat taaacaaatt tttgcttttt gtgtcatgca
     1021 tgctcttgtc gtttgtaacg tactcacaag acagttctaa tcctcagttt tctgactacc
     1081 tggttcctgt aagcaatgga ccatttgaaa agaatattca ctttaataaa gagcaggaaa
     1141 attactcgca acactggaag aatgcagtcc aagaggaact aaaaaagcct gttaattttg
     1201 cagggcactt tagaatctat acagcctttg gcggtcatgg taaagaatgc cttcgtgata
     1261 attgggtatg tggttgggtt attgataaac tatcagggga agttgtagca acacttccat
     1321 cagataataa tggaagttat aactatgctg atgtgtctga taatggtagc cctgttggtc
     1381 ttccatttga gattgatact tataaaaata gttcaatgat tgcaataacc ggtcaatcta
     1441 tctcagtaag caagagtgat aatcc
//
LOCUS       DAZGCQ010000302         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000302 DAZGCQ010000000
VERSION     DAZGCQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.305"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..>1453
                     /locus_tag="ACHK07_005336"
     CDS             72..>1453
                     /locus_tag="ACHK07_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8105502.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     AALSVAPEVTAIAEKIRVLDKELRRASVSLKTLKSKTVSSLGDFHAIDLTSKNGRELC
                     RTLAYKTFEK"
BASE COUNT          505 a          215 c          308 g          425 t
ORIGIN      
        1 taaataaaaa ttaaacaaaa attatatctc accactaagg tttataaaag cattcgttag
       61 caggtgtcat catgaaaaaa gccatagcat atatgcgatt ttcatcacca ggtcagatgt
      121 ctggcgactc attaaaccga cagagaagac ttattgctga atggttaaag gtaaatagtg
      181 attattatct tgataccata acatatgaag atttaggatt aagtgcattc aaaggaaagc
      241 atgcacaatc aggagctttt tcggaatttt tagatgctat agagcatggt tatatattgc
      301 caggaactac attgttagtt gaaagtctgg acagactttc aagagaaaaa gtcggtgaag
      361 cgattgagcg tctgaaattg attttgaatc acggtattga tgttataact ctttgcgata
      421 atacagtcta taatattgac tctttgaatg atccatattc attaataaaa gccatactta
      481 tagcacaaag ggcaaatgaa gaaagcgaga taaagtcaag tcgggttaaa ttatcatgga
      541 agaaaaaacg gcaggatgca ctggagtcag gcacgattat gacggcgtct tgtccgagat
      601 ggctctcatt ggatgacaaa agaacagctt ttattccaga ccccgacagg gtgaaaacga
      661 ttgagctaat ttttaaactc aggatggaaa ggcgatcatt gaatgcaata gccaagtatt
      721 taaatgatca tgctgtaaaa aatttctcag gaaaagaaag tgcatggggg ccttctgtaa
      781 ttgaaaaatt attagcgaat aaagctctga taggtatatg cgtaccttca tatcgtgcaa
      841 gaggtaaagg aataagtgaa atcgctggct attatcccag agtcatatca gatgatttgt
      901 tttacgctgt gcaggaaatt cggttggcac cttttggtat tagcaatagt agcaaaaatc
      961 ctatgttgat aaatctactt cgaacagtta tgaagtgcga ggcttgtggt aataccatga
     1021 ttgttcatgc ggtatctgga agtttgcatg gctattatgt ttgtccgatg agaagactgc
     1081 atcgatgtga caggccatca ataaagagag atttggttga ttataatatc attaatgagt
     1141 tgctttttaa ttgtagtaaa atccaaccag ttgaaaacaa gaaagatgct aatgaaactt
     1201 tagagttgaa aattattgag ctccagatga aaattaataa tttaattgct gcattatctg
     1261 ttgcgcctga agttaccgct atagcagaaa aaatcagagt attagataag gaattacgaa
     1321 gggcttctgt atcattaaaa actttgaaga gtaaaacggt gagctcactt ggtgattttc
     1381 atgctattga cttaaccagt aaaaatgggc gagagctatg tcgtacactt gcctataaaa
     1441 cattcgaaaa aat
//
LOCUS       DAZGCQ010000303         1396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000303 DAZGCQ010000000
VERSION     DAZGCQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.306"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1396
                     /locus_tag="ACHK07_005337"
     CDS             <1..>1396
                     /locus_tag="ACHK07_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8105503.1"
                     /translation="KDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAF
                     GCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGR
                     ILSIDAASRTLTLDREVILPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDG
                     METYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPMSG
                     SLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLVTS
                     AITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQ
                     ITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQWSVSGPHIKPGKDFW
                     FYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEM
                     AEMKTTITETRNEIT"
BASE COUNT          307 a          391 c          449 g          249 t
ORIGIN      
        1 tgaaggaccg gcacacggcg gtggaggtga attacaccga cccgcagaac ggctggcaga
       61 cttccacgga actggtggaa gacccggacg ccatcctgcg ctacgggcgc aatctgctga
      121 agatggatgc gttcggctgt accagccgcg gtcaggccca ccgtgccgga ctgtgggtga
      181 taaagacaga actgctggaa acgcagacgg tggatttcac gctcgggtca caggggctgc
      241 ggcacacgcc cggtgacatc attgaaatct gtgataacga ctatgccggg accctgaccg
      301 gcggacgcat cctgtccatc gatgccgcca gccgtaccct gacgctggac cgtgaggtga
      361 tactgccgga agcagggaca tcgacggtga acctgattaa cggcagcggt aagccggtgc
      421 gcgtggacat cactgcacac cccgcccctg accggataca ggtcagcgtc ctgccggatg
      481 gcatggagac atacggtgtg tggggactct ccctgccgtc actgcgtcgt cgcctgttcc
      541 gctgtgtttc catccgggaa aacacggacg gtacctttgc catcacggcg gtgcagcatg
      601 tgccggaaaa agaagccatt gtggataacg gggcccgctt tgagccgatg tccggctcac
      661 tgaacagcgt catcccgccg gcggtgcagc accttacggt ggaggtgagt gcctcagacg
      721 gccagtatct ggcactggcg aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca
      781 gtctgcgcct gaccagtggc agtggtgaaa acagccgcct ggtgaccagc gccatcacgg
      841 cggacacgga gcaccgtttc agtggcctgc cgcccgggga atacaccctg acggtcaggg
      901 cgataaacag ctacggccag cagggcgaac ctgccaccac cacgttccgg attaacgcac
      961 cggcagcacc ggccagcatt gaactgacgc cggggtatta tcagataacg gcggtcccgc
     1021 atcttacggt gtatgacccg acggtacaat ttgagttctg gttttcggag gcaaaaatcg
     1081 cagacacatc tcaggtggaa acctctgccc gttatctggg gacaggccgc cagtggagtg
     1141 tctccggccc gcacattaag ccggggaagg atttctggtt ttatgtgcgc agcgtcaacc
     1201 tggtggggaa atctgcgttt gtggaagcca gtggccgggc gagcaatgat gcggaagggt
     1261 atctggactt tttcagagga gaaatcggga agacacatct ggcacagggg atgtgggagc
     1321 tgattgataa cagccagctt gatgatgaga tggcggagat gaagaccacc atcaccgaaa
     1381 cccgcaatga aatcac
//
LOCUS       DAZGCQ010000304         1389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000304 DAZGCQ010000000
VERSION     DAZGCQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.307"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1389
                     /locus_tag="ACHK07_005338"
     CDS             <1..>1389
                     /locus_tag="ACHK07_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8105504.1"
                     /translation="VAGYFIEHKSRNSLLFQPTDSAAEDFMKSHVESTIRDVPCLKKL
                     SPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKE
                     GSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCGEEQY
                     LKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGL
                     TFFSAAGNEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALKDPNGVKTFVNTTLGET
                     WEEAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFL
                     VDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKK
                     HGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADE
                     ATSYAIRFPDDPE"
BASE COUNT          328 a          335 c          406 g          320 t
ORIGIN      
        1 gtcgccgggt attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat
       61 tctgccgctg aagattttat gaaatctcac gtggaatcca cgattcgcga tgttccctgc
      121 ctgaaaaaac tttctccctg gctgggacgt aaacatcgtg ataataccct cacgctgaaa
      181 cgcttttcat cgggtgtggg tttctggtgc ctgggtggtg cggcagcaaa aaactaccgt
      241 gaaaaatctg tggatgtggt ctgctatgac gaactttcct cgttcgagcc ggatgttgaa
      301 aaagagggct cgccaaccct gctgggggat aaacgtattg agggctcggt atggccaaaa
      361 tccattcgcg gctcgacgcc taaaataaaa ggttcctgcc agatcgaaaa agcggccaac
      421 gagtcggcgc atttcatgcg tttttatgtg ccctgcccgc actgtgggga ggagcagtat
      481 ctgaaatttg gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccccgaa
      541 agcgttttct acctctgtga acatcatggc tgcgtgatcc atcagtctga gcttgaccag
      601 agcaacgggc ggtggatctg tgaaaacacg gggatgtgga cccgtgatgg tctgacgttt
      661 ttcagcgctg cgggtaatga aattccgccg ccgcgctcca tcacgttcca tatctggacg
      721 gcgtacagtc cgttcaccac ctgggtacag atagtctatg actggctgga tgcactgaaa
      781 gatcccaacg gcgtgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc
      841 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcgtta cacggcggcg
      901 gtgcctgccc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
      961 atgtatgtct ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt
     1021 atggggcgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa
     1081 taccgccatg cagacggaac cgaaatgacc atttcccggg tctgctggga caccgggggg
     1141 atcgatggcg aaatcgttta tcagcggtca aaaaaacacg gtgttttccg ggtgctgccg
     1201 gtaaaaggcg catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag
     1261 cggggcgtgt atctgtgtga agtggggacg gacactgcaa aagaaattct ctatgcccgt
     1321 atgaaagccg atcccacgcc tgcggatgag gccacgtcgt atgccatccg ttttcctgat
     1381 gatccggag
//
LOCUS       DAZGCQ010000305         1389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000305 DAZGCQ010000000
VERSION     DAZGCQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.308"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1389
                     /locus_tag="ACHK07_005339"
     CDS             <1..>1389
                     /locus_tag="ACHK07_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8105505.1"
                     /translation="VVGYFIEHKSRNSLLFQPTDSAAEDFMKSHVEATIRDVPCLKKL
                     SPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKE
                     GSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCGEAQY
                     LKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGL
                     TFFSAAGNEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGET
                     WEEAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFL
                     VDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKK
                     HGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADE
                     ATSYAIRFPDDPE"
BASE COUNT          326 a          339 c          403 g          321 t
ORIGIN      
        1 gtggtcgggt attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat
       61 tctgccgctg aagattttat gaagtctcac gtggaggcga cgattcgcga tgttccctgc
      121 ctgaaaaaac tttctccctg gctgggacgt aaacatcgtg ataataccct cacgctgaaa
      181 cgcttttcat cgggtgtggg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt
      241 gaaaaatccg tggatgtggt ctgctatgac gaactttcct cgttcgagcc ggatgttgaa
      301 aaagagggct cgccaaccct gctgggggat aagcgtattg agggctctgt atggccaaaa
      361 tccattcgcg gctcgacgcc taaaataaaa ggttcctgcc agatcgaaaa agcggccaac
      421 gagtcggcgc atttcatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat
      481 ctgaaatttg gcgatgagtc cacgcctttt ggccttaaat gggagaagga cagccccgaa
      541 agcgttttct acctctgtga acatcatggc tgcgtgatcc atcagtctga gcttgaccag
      601 agcaacgggc ggtggatctg tgaaaacacg gggatgtgga cccgtgacgg tctgacgttt
      661 ttcagcgctg cgggtaatga aattccaccg ccgcgctcca tcacattcca tatctggacg
      721 gcgtacagtc cgttcaccac ctgggtacag atagtctatg actggctgga tgcactgaaa
      781 gatcccaacg gcctgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
      841 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcgtta cacggcggcg
      901 gtgcctgccc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
      961 atgtatgtct ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt
     1021 atgggacgtc cagatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa
     1081 tatcgccatg cggatggcac cgaaatgacc atttcccgtg tctgctggga caccgggggg
     1141 atcgatggcg aaatcgttta tcagagatca aaaaaacacg gtgttttccg tgtgctgccg
     1201 gtaaaaggcg cgtctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag
     1261 cggggcgtgt atctgtgtga agtggggacg gacaccgcaa aagaaattct ctatgcccgt
     1321 atgaaagccg atcccacgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat
     1381 gatccggag
//
LOCUS       DAZGCQ010000306         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000306 DAZGCQ010000000
VERSION     DAZGCQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.309"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1384
                     /locus_tag="ACHK07_005340"
     CDS             <1..>1384
                     /locus_tag="ACHK07_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105506.1"
                     /translation="LKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDI
                     AVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGD
                     KLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKDTGGVIE
                     GNGQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGNVT
                     LSSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKTAI
                     DGVSTSMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNITTTAITANGDKGLKISLNND
                     LTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAVNAGKGGLT
                     DAAGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNA
                     EVTDGNIRVDN"
BASE COUNT          452 a          239 c          385 g          308 t
ORIGIN      
        1 ctgaaagatg accttgatgc cattaatccg acgtggacgc taaaggatga taagggaggt
       61 tctgcgacta ttaatggtgg gaaggataat aagaatggta acactttaac tgttaaaggt
      121 gatggagata ttgcagttac tgttaatgat aaaggaatga cgattgacgg aacaacgctg
      181 aaaaatgaaa taaaaaatga tgccatcaaa attaaaggtg gagatggtaa gacgctgacg
      241 aagagtcttg gtgacaatgt cgaaatcact ggcaaaggcc caataaaaac ccaggtggct
      301 ggagataaac tggagatttc agcagagaca gcttcgttgc ctgtaaatgg tggtaacggg
      361 aagattacag taaacaatgg tgatggcgat aaacttgtta ctgcgaaaaa cattgctgat
      421 gccataaacc agtctggctg gaaaattaaa gcggataaag ataccggtgg tgttatcgag
      481 ggaaatggtc agggaacaga aacactgata aaacccggtg atatggttac gctgaaagcc
      541 ggtaaaaatc tcagcgtgaa acaaagtaac ggtgatttta cattctctgt tattgatact
      601 ccgtcattca gcagtgttca gctgaacggt aatggaaaaa acggaaatgt aacgctctca
      661 tccgaaggta ataagctgac attgtcaggc ggtaatggtg ccggacaaaa tggtgttgtt
      721 atcagtggtg tggcaagtgg attgaataat aaggacctgg caggcctgaa agcagacgat
      781 gcggaaatga aaaatgccgc cagtgttgca gacctgaaaa cggcaattga tggtgtgtcc
      841 accagtatgc agggtgcagg tttcgcactg aaagattcta agggacagga agtgaaacag
      901 tctcttgggc aggcgattaa agtatccggg gacagcaata tcaccaccac agcaatcact
      961 gccaatggag ataaggggtt aaaaatatcc ctgaataatg acctgactgt gggggataag
     1021 gctcccggca gcatcaaagt taacggggct gatggtaagg aggctgtgag tctgaatggt
     1081 caggatggca cgattaaact gacatcctcg cctgctgcgg gtggaaagtc tgcgtcagca
     1141 accattgctg ttaatgcagg caaaggtgga ttaacagatg ctgccggtac cacgaagaca
     1201 cgtctgactt ataccccgga tggaaaatca ccggcagaag agctggcgac catgaatgac
     1261 ggtctttctt ttgagggtga tgacggaaag gttattcata aaaaactgaa tgaaaaactg
     1321 accatcaaag gtggtgcaga gaagaatgct gaagtgactg acggcaatat ccgggttgat
     1381 aatg
//
LOCUS       DAZGCQ010000307         1380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000307 DAZGCQ010000000
VERSION     DAZGCQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.310"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1380)
                     /locus_tag="ACHK07_005341"
     CDS             complement(<1..>1380)
                     /locus_tag="ACHK07_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105507.1"
                     /translation="YEKNNSKHSCDLISDIISEINKIDSKLILIEAPAGFGKTCTSYE
                     ILNSLISIDGAPIPFFTEFSRDRQAKVFSHVFVREVDRSFRTVKSDLVIDETKNGKIV
                     LVLDGFDELLNDTTSNKTNDIDFEKAQPMLETIGELLTKSAKVILTSRRAAIFDGEIF
                     QEWKDKYRDNFDVVRYRLNSPEVRDWLSHERIEQLEESGIDIKKLANPVLLAYLRSLE
                     NENFNNLCERSSKIVEHYFQAMLERERERQDLLMTPEKQSSILTMVALDMCENDYTSD
                     NRDKIISLIKEKCLFILEETRQLYTGKDRPTLDALASKLATHAFFDRSNQGENKIEFV
                     NEFVFGNYIANSIIANDSDDWLANDERFVEPAVQAYLPRDSHDRELLWTRLSGMREFL
                     SSSDHMKYEYLLTQEIDASIYSETTITGLSFDNALFFQHSEIKNSVFNECVFSNVVFN
                     FNNFDDVTF"
BASE COUNT          428 a          254 c          208 g          490 t
ORIGIN      
        1 atgaatgtaa catcatcaaa attgttaaag ttgaatacca catttgaaaa aacacactca
       61 ttgaatacag agttttttat ttcagaatgc tggaaaaata aagcattatc gaaagacaac
      121 ccagtaattg ttgtctcact gtaaattgag gcatcaattt cttgcgttaa taaatactca
      181 tatttcatat ggtcactcga actcagaaat tcacgcattc cacttaatcg tgtccatagt
      241 aattcacgat catgactatc tctcggtaaa tatgcttgaa cagctggttc aacaaagcgc
      301 tcatcattag ctagccaatc atcagagtca ttggcaatta tactattagc gatataattg
      361 ccaaatacga attcattgac aaattctatt ttattctcac cttgattgct tctgtcaaaa
      421 aaagcatgtg ttgctaattt acttgctaga gcatctaaag ttggcctgtc tttacctgta
      481 tataactgtc tggtttcttc taatataaaa aggcattttt cctttatgag agatattatt
      541 ttatctctat tatcacttgt atagtcattt tcacacatat ctaaagcaac catagtaaga
      601 atggatgatt gcttttcagg agtcataagc aagtcctgtc tctctctctc tctttccagc
      661 attgcttgaa aatagtgttc aacaattttt gaacttcttt cacatagatt attaaagttt
      721 tcattctcta atgaacgcag atatgctaaa agaactgggt ttgctaattt ctttatatct
      781 atgcctgatt cttcgagttg ttcaatacgt tcatgactta gccaatccct gacttcgggt
      841 gagtttaatc tatatctcac aacatcaaaa ttatctctat atttatcttt ccactcttga
      901 aatatctcac catcaaagat agcagctcta cgagacgtga ggattacttt tgcagacttt
      961 gttaataatt caccaattgt ttctagcatt ggttgagctt tttcaaaatc aatatcgttt
     1021 gttttatttg atgttgtatc atttagcaac tcgtcaaaac catcaagtac taaaactatt
     1081 ttcccatttt tggtttcgtc tattacgaga tcacttttaa ctgttcggaa acttcgatct
     1141 acttcgcgaa cgaaaacgtg gctaaaaact ttcgcttgtc tatcgcgaga aaattcagta
     1201 aaaaatggga tgggagcacc atcaattgat ataagggaat tcaatatctc ataggatgta
     1261 caagttttgc cgaatcccgc gggagcttca ataaggatta attttgaatc gattttgtta
     1321 atctctgata tgatatcaga gattaagtca caagaatgtt ttgaattgtt tttctcataa
//
LOCUS       DAZGCQ010000308         1374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000308 DAZGCQ010000000
VERSION     DAZGCQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.311"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1374
                     /locus_tag="ACHK07_005342"
     CDS             <1..>1374
                     /locus_tag="ACHK07_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105508.1"
                     /translation="LKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDI
                     AVTVNDKGMTIDGTKLTENIKTDAIKIKDNNGGLLKKDLSDEIEITGKGPIKTQVAGD
                     KLEISAETASLPVNGGNGKITVNKGDDDKLVTAKKIADAINQSGWKIKAGSENGGKVE
                     GGNKEELINPGDTVTLKAGKNLSVKQSGGDFTFSVIDTPSFSSVQLNGKGNNGSVTLS
                     SEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDG
                     VSSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSV
                     GSNNTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDA
                     AGTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNAEV
                     TDGNIRVD"
BASE COUNT          453 a          232 c          373 g          316 t
ORIGIN      
        1 ctgaaagatg accttgatgc cattaatccg acgtggacgc taaaggatga taagggaggt
       61 tctgcgacta ttaatggtgg gaaggataat aagaatggta acactttaac tgttaaaggt
      121 gatggagata ttgcagttac tgttaatgat aaaggaatga cgattgacgg aactaaactt
      181 actgaaaaca ttaaaacaga tgctataaaa ataaaagata acaatggtgg tcttctgaaa
      241 aaagaccttt cggatgaaat cgaaatcact ggcaaaggcc caataaaaac ccaggtggct
      301 ggagataaac tggagatttc agcagagaca gcttcgttgc ctgtaaatgg tggtaacggg
      361 aagattacag taaacaaagg tgatgacgat aagcttgtta ctgcgaaaaa gattgctgat
      421 gccataaacc agtctggctg gaaaattaaa gctggcagtg aaaatggtgg gaaagttgag
      481 gggggtaata aagaggagct aattaatccc ggtgatacag ttacactgaa agccggtaaa
      541 aatctcagcg tgaaacaaag tggtggtgat tttacattct ctgttattga tactccgtca
      601 ttcagcagtg ttcagctgaa tggaaaagga aataacggaa gtgtaacgct ctcatccgaa
      661 ggtaataagc tgacattgtc aggcggtaat ggtgccggac aaaatggtgt tgttatcagt
      721 ggtgtggcaa gtggactgga aggtaaggaa ctttcgacgt taacgaatac tgataaagag
      781 atgaaaaatg ccgccagtgt tgcagacctg aaaacggcaa ttgatggtgt gtcctccagt
      841 atgcagggtg caggtttcgc actgaaagac tctgatgata aggaagtgaa acagtctctt
      901 gggcaggcga ttaaagtatc cggggacagc aatatcacca ccacagccat caccaatggg
      961 gataaggggt taaaaatatc cctgaataat gacctgagcg ttgggagcaa taatactccc
     1021 ggcagcatca aagttaacgg ggctgatggt aaggaggctg tgagtctgaa tggtcaggat
     1081 ggcacgatta aactgacatc cgcgcctgct gcgaacggaa agtctgcgtc agcaaccatt
     1141 gctgttaatg caggcaaagg tggattaaca gatgctgccg gtaccacgaa gacacgtctg
     1201 acttatgctc cagatggaac attaccggca gaagagctgg cgaccatgaa tgacggtctt
     1261 tcttttgagg gtgatgacgg aaaggttatt cataaaaaac tgaatgaaaa actgaccatc
     1321 aaaggtggtg cagagaagaa tgctgaagtg actgacggca atatccgggt tgat
//
LOCUS       DAZGCQ010000309         1345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000309 DAZGCQ010000000
VERSION     DAZGCQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.312"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..436
                     /locus_tag="ACHK07_005343"
     CDS             56..436
                     /locus_tag="ACHK07_005343"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8105509.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            433..780
                     /gene="tnpB"
                     /locus_tag="ACHK07_005344"
     CDS             433..780
                     /gene="tnpB"
                     /locus_tag="ACHK07_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO8105510.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            830..>1345
                     /locus_tag="ACHK07_005345"
     CDS             830..>1345
                     /locus_tag="ACHK07_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO8105511.1"
                     /translation="MNDISSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRP"
BASE COUNT          321 a          384 c          368 g          272 t
ORIGIN      
        1 cgaattagac aaagtggtgt ccaccaaata agtagtggga accaaagtgt cagatatgca
       61 gaaaaatgtg actcccggca ggcgaaaagg ctgccctaat tatcctcccg aatttaaaca
      121 gcagctcgtt gctgcctcct gtgaacccgg gatatccatc tcaaaacttg ctcttgaaaa
      181 tggcattaac gccaatctgt tgttcaaatg gcgacaacaa tggcgcgagg gaaagctgct
      241 attaccttct tcagagagcc cccagctact tcctgtgact ctcgatgcag ctgccgaaca
      301 gccagaatcg ctcgcagagg acccggaaac cctcagtatc agctgtgagg taacgttccg
      361 gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc tgctgataca
      421 ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt tgccggtatc
      481 accgatatga gaaatggctt caacggcctg gctgcgaaag tacagacggc gctgaaagac
      541 gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca ggttaaactg
      601 ctgtggtcca ccggtgacgg gctgtgcctc ctgaccaaac ggctggagcg tgggcgcttc
      661 gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct ggcgatgctg
      721 ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac catgctgtaa
      781 atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta tgaacgacat
      841 ctcttctgac gacatcctcc tgctgaaaca gcgcctggcc gaacaggaag cgctgatcca
      901 cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc aggcgcagct
      961 ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc gccgtatcgc
     1021 acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga ctggtagggt
     1081 gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc cgttccctga
     1141 atcactaccc cgtgacgaaa agcgactgtt gcctgcagcg ccgtgctgcc cgaactgcgg
     1201 cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga tgcgtagcgc
     1261 cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg atgccatcgt
     1321 gcaggcacct gcaccttcgc ggccc
//
LOCUS       DAZGCQ010000310         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000310 DAZGCQ010000000
VERSION     DAZGCQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.313"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1103
                     /locus_tag="ACHK07_005346"
     CDS             84..1103
                     /locus_tag="ACHK07_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFO8105512.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          381 a          303 c          375 g          282 t
ORIGIN      
        1 caggtgtgga gcctcgcaga tttccggcat catagtgccc aatacgggat gcacaaagct
       61 ccctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat atcgcaaaac
      121 atgtcattca gattcacttc atcaatgagc acacaggtga agtggttgat aaacagttgc
      181 gtagacagga ttttctgacg ttcttcggca accgtgagcc atgcctgatt ggtatggagg
      241 cctgtggagg ttctcagcac tgggcacggg aactgacaaa acttggtcat aaagtccggt
      301 tgttgcaggc ccgcttcgtt aaggcattcg tcatgggcaa taagaatgat gtgatggatg
      361 ccagggctat ctggatggcg gttcagcagc cgggtaaaga aatcgccgta aaaacagaag
      421 aacagcagtc ggtactggtt ctgcaccgta cccgcatgca actggtgaag ttccggaccg
      481 cacaaattaa tgccctgcac gggacgttac tggagtttgg tgaaaccatc cacaaaggcc
      541 gggcagcgat ggagcgggag ttccccgaag cactggaacg gatgaaagag agactgccac
      601 cgtatctcat tatggttctg gaaaaccagt acaaccgact gaatgagctg gactcactga
      661 tagaggatat tgaaaaacag cttaccagcg tggcgaggca gaatgaaacc tgtaagcggt
      721 tgctggatat tcctggcgtt ggaccactta ttgcgacggc agcggtggcc accatggggg
      781 aagcatcagc gtttaaatcg gggcgagagt tcgccgcata tgttggtctg gttccaaaac
      841 aaactggctc cggagggaaa gtacgtctgc tggggataag caaacgtggt gacacttatc
      901 tcaggacatt atttatccac ggtgcaagag cggtggcatt agtagctaaa gagcctggcc
      961 cgtggataac cgaactgaaa aaacgtcgtc cagccagtgt ggcaatcgtc gccatggcaa
     1021 acaagctggc acgaacagta tgggcgataa ccgcccatga ccgtaagtat gacaggaacc
     1081 acgtcagtat cagaccatat taatcgctga taccgttaaa caatgaactc ttaacaaaag
     1141 ggtgaatgct gaaaggttgc tatggcggcc agagtgatga caaagacagg taagaccgtg
     1201 actcactaaa cctgaacagt attttgggct tgaagtccgc cgtgaaaata aggggtgagt
     1261 cggcgaatta cataggggct cgcagcgtta cggctgcaat aaagccggat ataaagctgc
     1321 aacctacccg tcatgtcaaa a
//
LOCUS       DAZGCQ010000311         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000311 DAZGCQ010000000
VERSION     DAZGCQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.314"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1341)
                     /locus_tag="ACHK07_005347"
     CDS             complement(<1..>1341)
                     /locus_tag="ACHK07_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8105513.1"
                     /translation="LNDGLIKRVLNLFIHLISNPDNLELTLNRYSSTTEQIIGRTKRN
                     GLHEFDVGDLKIIFNRQDDNESVLTVKYKDKDISHHCNVKTEQLQQFIKIMEQKAQLS
                     IYIDKNNLKESIFSVLHNDPQHVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHL
                     NNFMISFDPVENQLTIRDNNNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDN
                     IDKIKNTISTSEIIECASPEIGSSVLNDLYSIANFLPDNNLTPNESWKRFCDTCERFY
                     VAQKSITGDKSERLTRKLSISDAGITMTFKIGDVVINTIITAIPEDATGQRCIEGLNL
                     AEMDLTGIDLSKMVLRNVNFNGSILRHAKFSGTICEGVDFTDCDLRNAKFKNASLENN
                     DFRKVRHLTYVNFKNANLRNSNFSGKVLTGVNFTGSNLSNAYLEYIDFTTVILY"
BASE COUNT          384 a          228 c          244 g          485 t
ORIGIN      
        1 catatagaat cactgttgtg aaatctatat attcaagata agcgttgcta aggttacttc
       61 cagtaaaatt aacgccagtg agaacttttc cgctgaagtt actgtttcgt agatttgcgt
      121 ttttgaaatt tacataagtc aagtggcgaa ctttacgaaa atcattattt tctaatgagg
      181 catttttaaa ttttgcatta cgcagatcac aatctgtaaa atccacgcct tcacagatcg
      241 taccggagaa cttggcatgt ctaagaatgc tgccattaaa attgacattc cttagaacca
      301 ttttagacaa gtctatgccg gttaaatcca tttctgctaa attcaaccct tcgatacacc
      361 gttgacccgt tgcatcttca ggaatagcag tgataatagt attgatgaca acatcaccta
      421 tcttgaaggt cattgtaatt cctgcatcag agatagagag ttttcgcgta agacgttcac
      481 tcttatctcc agtgatactc ttctgagcaa cgtaaaagcg ctcgcatgtg tcacaaaatc
      541 ttttccagct ctcatttggg gtcagattat tgtccgggag gaaattagca atgctataaa
      601 gatcgttcag gacgctactt cctatttcag gagaggcgca ttcaataatt tcagaggtgg
      661 atattgtatt tttaatttta tctatattat ccgtgagatt tcgtgatcct gctatatttg
      721 gctgctggtg gttttctttg tagtattgca gcaaattttc ccattgtaag tttgtaaacg
      781 agaaagtttc agtcttgtta ttgttatccc gtatggttaa ttgattttct actgggtcaa
      841 aagaaatcat gaagttattc aggtgttgat attgatgagt ggcatctaat ttcacttcaa
      901 atgaatttga acttttgcag gcatgtttta aaaacttttc acagggaagg tgttgatctt
      961 tatctacgtg ttgtgggtca ttgtgcaaaa cagagaaaat actctctttc aaattgttct
     1021 tgtcaatata gattgatagt tgcgcttttt gttccattat tttaataaac tgctgcagtt
     1081 gctcggtttt aacattgcag tgatgactta tatctttatc tttatattta acagttaata
     1141 cgctttcatt atcatcttgt cgattaaata ttatttttag atcgccaacg tcaaactcat
     1201 gtaaaccatt tcttttggtt ctgccgatga tttgttctgt tgttgatgaa tatctattta
     1261 aggttaattc aagattgtcg ggattagaga taaggtgaat aaaaagattc agtacacgct
     1321 taatcaaacc atcattcaaa t
//
LOCUS       DAZGCQ010000312         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000312 DAZGCQ010000000
VERSION     DAZGCQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.315"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /locus_tag="ACHK07_005348"
     CDS             complement(<1..216)
                     /locus_tag="ACHK07_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO8105514.1"
                     /translation="MNNTLLPSINIPCTLFETISLFNDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAF"
     gene            403..549
                     /locus_tag="ACHK07_005349"
                     /pseudo
     CDS             403..549
                     /locus_tag="ACHK07_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(592..972)
                     /gene="yigF"
                     /locus_tag="ACHK07_005350"
     CDS             complement(592..972)
                     /gene="yigF"
                     /locus_tag="ACHK07_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFO8105515.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(986..>1325)
                     /gene="yigG"
                     /locus_tag="ACHK07_005351"
     CDS             complement(986..>1325)
                     /gene="yigG"
                     /locus_tag="ACHK07_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFO8105516.1"
                     /translation="RRRYIFSFILINFIFSFLIIFFNDGEAGFLVIVSTIVLHYLVIN
                     MNCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSGNGSMIFLICYFSTFSML
                     MLAPTDSSKQ"
BASE COUNT          464 a          229 c          234 g          398 t
ORIGIN      
        1 aaaagcaaca ttaatagaac ctgggccagg gaaatggtat ttaataagtc tgtaaggatc
       61 aacttttgct gagatatcgc ttaggccgag tgagagaaaa tcttgttcta ccatatcacc
      121 atattgcata tcatcagcag aataatcatt aaataaacta atagtttcga aaagtgtaca
      181 gggaatatta attgatggaa gtaatgtgtt attcatttac attttccttg gttatattta
      241 agctcaatat caatttgaga acaaatgcta gggtaaaaaa atatttaaaa aagaagtaca
      301 cttcacattt tttcattaca tctctgaaga gaacgtcgtt aatgattggt cttgctcatt
      361 gttaataaca aaatattgaa tttaccgatt aggaaacttt ttcctacgcc gattgcctcc
      421 agctatggca tgaactttga gtttatgcca gaactgaagt ggagtttcgg ctacccaggc
      481 gcgattatct ttatgatcct cgcgggcctg gcaccgtatc tgtactttaa gcggaagaac
      541 tggttgtaaa aaacgagagc ggtggtttag cctggctaag ccacctgtta ttcaaaggct
      601 ccaggtattt aaccctttta cctctttctc ataaaaccat ttgttcgtgt taacagcaac
      661 ataggctgct acggcaattc ccagaatgtt aacgccaatt aatgcaccaa caaatagaga
      721 tagaatgcca agtaatagaa ctataattgc ttttttccat aagcccagaa caaataaata
      781 gatccaggaa cagaagaaag caatgaaatt catttgaata gttaatcgtt gtcttacttt
      841 taatgcttta aatgctgctt tatattctgg tgttgctccc cagaatccag gaaagccatg
      901 ttgatcataa aaattaaatc ggtatttcca tttttcactt aatgaaccat cgttcatata
      961 ttccttactc ataaatactc cataactatt gttttgatga atcagtaggt gcaagcatta
     1021 gcatactgaa agtggaaaaa taacaaatca aaaaaatcat cgaaccattg cctgaacagg
     1081 caaaatcttc ggctatcatt gtgatgatag agatgatata tactgctaat gtaccaaaaa
     1141 cataagtttt tatatagatg aaaccactat cacggagtcg ctggcaattc atgttgatga
     1201 cgagataatg gagtacgatg gtagagacta taacaagaaa acctgcttcc ccatcgttaa
     1261 aaaagataat aagaaaggaa aaaatgaaat ttattaaaat aaatgaaaat atatatcggc
     1321 gtctg
//
LOCUS       DAZGCQ010000313         1274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000313 DAZGCQ010000000
VERSION     DAZGCQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.319"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..>1274
                     /locus_tag="ACHK07_005352"
     CDS             18..>1274
                     /locus_tag="ACHK07_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO8105517.1"
                     /translation="MLVSKSNELNTSAFFASRNYNGNNSSNPMELLAHSIIKLICKEA
                     ASATYCGALETLQKMMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENAASEVHKSANKGEIEQLVECSKRNE
                     QTLFDNIRKSDFHVGSLKPGSMNNVILEMSPNVCMEPRNSYENKIDEVSSLSEPKEHT
                     IDIQEKTDAFVKKFKGVLFDKNTRSSELLFNFYECCYVFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLIHNNIGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRNKKIKMITGVAPFD"
BASE COUNT          445 a          189 c          255 g          385 t
ORIGIN      
        1 taattgagga atagacaatg ttagttagta aaagcaacga acttaacact agcgcatttt
       61 ttgctagcag gaattataat ggaaacaatt cttccaaccc catggagcta ctagctcata
      121 gcattataaa attaatttgt aaggaagctg cctcagcgac gtattgcggt gctcttgaaa
      181 ctttacaaaa aatgatgtct gaatgtatat atcaagaagg caacgccttt gtcattatgg
      241 gagctggaga acaattaaaa cgtattaaat atgatgttga tgaaaataac ttaaaggtat
      301 tcaacgtaca ctttgataat aatgaagtgt tagttactga tggtgagcct gacgtagtat
      361 gtttaagcaa gcaggtctgg gaaaatcttc tcattaaatt aaaaccggag atcaaggaaa
      421 atgcggcttc tgaagttcat aaatcagcga ataaaggtga gattgagcaa ttagttgaat
      481 gctctaaaag aaatgaacag acccttttcg ataatataag aaaaagtgat tttcatgttg
      541 gttcacttaa gccaggtagt atgaataatg ttattttaga aatgtcacca aatgtctgta
      601 tggaaccacg taattcatat gaaaacaaaa tagatgaggt ttcatctttg tcagagccaa
      661 aggaacacac catagatatt caagaaaaaa cagatgcgtt tgtgaagaag ttcaaggggg
      721 tattatttga taaaaataca aggtcttcag agcttctgtt taatttttat gaatgttgct
      781 atgtgttttt accaagagcg cagcctcagg ataaaatcga tagctataat tcagcactgc
      841 aagctttttc catcttttgt tcatctacgt tgatacataa taatataggc tttgatttca
      901 aattatttcc agaagtcaag ctgtctggag aacatcttga aacggtattc aaatacaaaa
      961 atggcgatga tgtccgggag atagccaaaa ttaacattac tctccaaaaa gaagagggtg
     1021 gcttatataa tctacgtgga ttggatttta aggggtgctt cttttctgga cagaacttca
     1081 gtaactatga tattcaatat gtgaactggg gaacgtcatt gtttgatgtt gatactccgt
     1141 gtatttttaa tacgcctgct aaccatgaga gttatgaaaa atcattaaaa cctgtaagcg
     1201 aaaacggttt aaatggagtc ttatctgatc gtaataaaaa aataaaaatg atcacgggtg
     1261 tggcaccatt cgat
//
LOCUS       DAZGCQ010000314         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000314 DAZGCQ010000000
VERSION     DAZGCQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.320"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHK07_005353"
     CDS             complement(<1..416)
                     /locus_tag="ACHK07_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO8105518.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEP"
BASE COUNT          383 a          288 c          254 g          347 t
ORIGIN      
        1 ggttcactga acagcggact ccactcggtc accccgtttt cctgcaatat cgtgccgagg
       61 atgctttcga tgtcctcatt ctggaagata cggaagttct ggcgcagccc tgtgcgccac
      121 agcggtgggc acaccttcat gctgtacagc atctggtttt tgtcgttctc ccccagttca
      181 aaccaggtca ccacaccttt cacccggcgc tgtacgtcat cgccctgcca taccgtcagg
      241 taggccattt tgtccagcac ctgcgcaaat tcaagggaaa gaaactgctg actgaccaga
      301 gaaaggtcga gggaaaaaag tgaagagagt gactgggtga gatgaaagga aaccaccgca
      361 aaagcatccg gtggcaggcc gtccacttcc agcgtgaaac gtaatccggt tgacatagca
      421 acctccgtga aaatgaataa taatccgcac atattgcatg tacaacatgt cgggcggatg
      481 agataagcga gtcgacatct ggcgttaccg gggaatatca gaaaaatgtc atggttatgt
      541 gatgatttag tctgggattt tacttcagag aaacgggtgt ctgtttagtc aacaaaataa
      601 tcacaaacgc aacggatggg ttctcgacgt tattcacgac aggctcgtac attacagcga
      661 gtaataaaaa acctgatatg caaagtcaaa ccaatattca taatgtcttt ttattcacaa
      721 caataaataa tttaataaca atcattgaaa tgaatgatga tttgtataag gaatatacgc
      781 taccgcccca ccctgataac atgatctgca cattgccatc ttaacgtcgt taaacactgg
      841 cttaatagtg gatatttcaa attaggaatt tattaaattc acctgatgaa tatcataagc
      901 aaatagcata gaaaataatg acaactcccc cgaggggacc atttccaaag cactgcacca
      961 ccacctggtg cccggatgat cttgcttcag tggtaagagt acctgatgca ttgttcccaa
     1021 aatggtgttt tcccctgccc ctccaataat gtgcagctct cacgtgataa tctattgatt
     1081 aggttatatt aagaatcaat atttaaaata catccatttt cttgaataaa tcaacatgtt
     1141 tattaatcaa ttgctgtttt agctctgact catcaagctc tccatatttg tcctacagat
     1201 aattgcgaat aaatacagag aacgccattt atccttttag catacctgcc ggatattttt
     1261 atttgtatta at
//
LOCUS       DAZGCQ010000315         1270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000315 DAZGCQ010000000
VERSION     DAZGCQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.321"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1270
                     /gene="espI"
                     /locus_tag="ACHK07_005354"
     CDS             <1..>1270
                     /gene="espI"
                     /locus_tag="ACHK07_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO8105519.1"
                     /translation="FGTESGKEGILSEKASRGVAVGLLSGFNTAYRGAIHAPSASATV
                     NNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNA
                     DKLVVNKKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVT
                     PVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRD
                     INGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSAS
                     ADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWY
                     AGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGV
                     DVGKSFSGKDWKVTARAGLGYQFDLLANGET"
BASE COUNT          342 a          282 c          351 g          295 t
ORIGIN      
        1 gcttcggaac tgaatcaggt aaagagggca tattgtctga gaaggcatcc cggggagttg
       61 cggtcggatt actgagtggt tttaatacgg cataccgcgg tgcaattcat gccccgtctg
      121 catctgccac tgtgaacaat acctggtggc aactgacagg agactcctca cttcgctcgt
      181 taaaaaatac cggaagcatg acatatttta caggaagtgc agcgaataaa gcattccata
      241 cactgacggt tgatgagctg acgacgaatg gcactgcgta tgccatgcgt acggacctga
      301 aaaatgcgga taagctggta gtaaataaaa agctgtcagg taaggacaat attctgctgg
      361 ttgattttct gaacaaaccc accggagaaa aactggatat tgaactggtg agtgcaccgg
      421 ggaacagcag taaggatgtt tttaaaggaa gtgaacaggc aataggtttc agcaatgtca
      481 cacctgttat tacagctata gacgccggag ataaaacaac atggaacctg accgggtaca
      541 ggatggcaga aaatcctgcc gcaacccaaa gtgcctcagg ccttgcatct gtggggtaca
      601 aatcattttt gagtgaggtc aacaacctga ataaacgtat gggtgacctg cgtgacatca
      661 atggtgaagc tggcgcatgg gcacgtatca tgagcggaac cggctctgcc ggtggtggtt
      721 tcagtgacaa ccacacacat gttcaggtcg gtgtcgacaa aaaacatgag ctggacggac
      781 tggatttgtt taccggcttc actgtcacac acactgacag cagtgcctcc gctgatgctt
      841 tcaaaggtaa aacgaagtct gtgggggccg gactctatgc ttctgccatg tttgattccg
      901 gtgcctatat cgacctgatt ggtaagtatg ttcatcatga taatgagtac acggcaacct
      961 ttgccggact cggaacccgt gattacagta cgcattcatg gtatgccggt gctgaagcag
     1021 gctaccgctg tcatgtcact gaggatacct ggattgagcc acaggcagaa ctggtttacg
     1081 gtgctgtatc cggtaaacag tttgcatgga aggaccaggg gatgcatctg tccatgaagg
     1141 acagggacta caatccgctg attggtcgta ccggtgtgga tgtgggtaaa tccttctcag
     1201 gtaaggactg gaaagtgaca gcccgtgccg gtctgggcta ccagttcgac ctgctggcta
     1261 acggcgaaac
//
LOCUS       DAZGCQ010000316         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000316 DAZGCQ010000000
VERSION     DAZGCQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.322"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..561)
                     /locus_tag="ACHK07_005355"
     CDS             complement(<1..561)
                     /locus_tag="ACHK07_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO8105520.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQ"
     gene            622..810
                     /locus_tag="ACHK07_005356"
                     /pseudo
     CDS             622..810
                     /locus_tag="ACHK07_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1178..>1265)
                     /locus_tag="ACHK07_005357"
     CDS             complement(1178..>1265)
                     /locus_tag="ACHK07_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8105521.1"
                     /translation="LGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          333 a          301 c          301 g          330 t
ORIGIN      
        1 ctgctgagat ggctgactaa atgattgatt agctaaccag gttcgatgtt tgccggtcca
       61 gtgcttccct gcataacgtt tgtctgttcg caatagcatg ctcagaattc gctgtcgggc
      121 aactctgctg tctcgtttag ctgctgcacg ggctctgatt agatcccgca tagcttcatg
      181 agtcaggtca gggatccaga caggggtaag ttcaccagcc cgcagtaacc tggcaagaga
      241 aattgcgtcc cgatgatcat ttttgattct gtctgtcggt gattttggta tccgggaagg
      301 ggcaataacc tggcatttta tattcatcgc tgtgagttga tgatatagcc caaaaccaca
      361 ggggccagcc tcatagcagg aaagaatgtt tccgtaaagt ccctgcaatt tctgaaataa
      421 tcgacgtaca gcctgggctt cgttgttaat cgtaccataa taacgaactt cgccaagccg
      481 ttcaggtgca gcaatagcaa ccgccaacgt ttctttatgt acatccagcc cgatgtaagc
      541 tgcaatattt tccgaatgca taatgacctc ctctggaata tcccaccaat gctatagtga
      601 gtatataaag gtcataccat ctttatgttc tttggcagcg gtcacggcgg cgagcgtgga
      661 gcactgttgt acgggctgat cggcacctgc cgtctgaacg gtatcgatcc ggaagcgtat
      721 ctgcgccata tcctgagcgt actgccggaa tggccttcca accgagttga tgaactcttg
      781 ccatggaacg tagtactcac caataaataa gcgtcaatac ggtgctccgt tgacgcttac
      841 gatcaaaggg cgtatatcac ggcgtcttcc ggtaaggaac tcttctggca ttggcgttga
      901 attattacct gtggagatga cgctggatga gcggccttac cccgcttttt acgcgaggtg
      961 attatgaaga tcaatgacta gccgtcgaac cgtgtgcatg aactgctgca ctgaaacttc
     1021 tcagctgtaa aataatcctt acgctacgta cttcccggag cgcttacgat taatcggatg
     1081 cagtcctgac tggaagaaag aaaaatggga aaaaataaga ggaattaata tcctccacgt
     1141 tcctgaaatc ctggaggata ttaatgtcat caaaatatta gcgaacgata atccctaaca
     1201 aaatacccag tgcgccaaag tcagcatcac tgaaggtggt gttagcaaaa ccaatgttac
     1261 ccagc
//
LOCUS       DAZGCQ010000317         1262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000317 DAZGCQ010000000
VERSION     DAZGCQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.323"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1262
                     /locus_tag="ACHK07_005358"
     CDS             <1..>1262
                     /locus_tag="ACHK07_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO8105522.1"
                     /translation="QNGTISLRVRPDMAQSNADRLVIDGGRATGKTILNLVNAGNSAS
                     GLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAYRA
                     EVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIVRGAT
                     PESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDSSRAGTVRDDAGS
                     LGGYLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRVRGRGWLGSLETGLPFSITDN
                     LMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRD
                     TLRGSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQ
                     AGLEARVRENITLGVQAGYVHSVSGSSA"
BASE COUNT          276 a          354 c          393 g          239 t
ORIGIN      
        1 cagaatggca ccatcagcct gcgtgtacgc ccggatatgg cacagagcaa tgctgacaga
       61 ctggtcattg acggcggcag ggcaaccgga aaaaccatcc tgaacctggt gaacgccggc
      121 aacagtgcgt cggggctggc gaccagcggt aagggtattc aggtggtgga agccattaac
      181 ggtgccacca cggaggaagg ggcctttgtc caggggaaca ggctgcaggc cggggccttt
      241 aactactccc tcaaccggga cagtgatgag agctggtatc tgcgcagtga aaatgcttat
      301 cgtgcagaag tccccctgta tgcctccatg ctgacacagg caatggacta tgaccggatt
      361 ctggcaggct cccgcagcca tcagaccggt gtaaatggtg aaaacaacag cgtccgtctc
      421 agcattcagg gcggccatct cggtcacgat aacaacggcg gtattgtccg tggggccacg
      481 ccggaaagca gcggcagcta tggcttcgtc cgtcttgagg gtgacctgct cagaacagag
      541 gtcgccggta tgtctctgac gacaggggtg tatggtgctg caggccattc ttccgttgat
      601 gttaaggatg atgacagctc ccgtgccggc acggtccggg atgatgccgg cagcctgggc
      661 ggatacctga atctgataca caacgcctcc ggactgtggg ctgacattgt ggcccaggga
      721 acccgccaca gcatgaaagc ctcatcggac aataacgact tccgcgtccg gggccggggc
      781 tggctgggtt cactggaaac cggtctgccc ttcagtatca ctgacaatct gatgctggag
      841 ccgcaactgc agtacacctg gcagggactc tccctggatg acggtaagga caacgccggt
      901 tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg ccggcttccg tctgggcagc
      961 cacaacgata tgaactttgg taaaggcacc tcatcccgtg acaccctgcg cggcagtgcc
     1021 aaacacagtg tgcgtgaact gccggtgaac tggtgggtac agccttctgt tatccgcacc
     1081 ttcagttccc ggggggacat gagcatgggt acagccgcag ccggcagtaa catgacgttc
     1141 tcaccgtcac agaatggtac gtcactggac ctgcaggccg gactggaagc ccgtgtccgg
     1201 gaaaacatca ccctgggcgt tcaggctggt tatgtccaca gcgtcagcgg cagcagcgct
     1261 ga
//
LOCUS       DAZGCQ010000318         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000318 DAZGCQ010000000
VERSION     DAZGCQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.324"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..>1258
                     /locus_tag="ACHK07_005359"
     CDS             111..>1258
                     /locus_tag="ACHK07_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO8105523.1"
                     /translation="MVDNVTVGGVCAQSPSFMPDLDGGKNKAQLIIDDIVAYLKNPSV
                     YSLEKEGPLNHFVNNCSEIEPWVLIGGGLIIIVSTSEQDPEGLIVTVGDIEGKCATQI
                     PVPPALTTSLKDIFIRPHTNAKYESFTKEQIKANIKSNVINYNCKLHFNEKPENEIVC
                     RHLSLQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFDNLQKNPPPGTCVVA
                     CDKFGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLRSFMNIKQAQKKWVITEICSDCVGITPYLPREQAHLLSG
                     INNELQPPLSPSALHLLIQMGMHENIVLFFDKFKNSQEMTASKVLAAKS"
BASE COUNT          432 a          222 c          231 g          373 t
ORIGIN      
        1 taatgacgta aaaagacaaa taactctata aatgagcgaa atttgatagt tgatttttaa
       61 atagtaacct tattattact acaactttca tatggatgag gggaaagaaa atggttgata
      121 acgtaactgt tggcggtgta tgtgcacaat ctccttcttt catgcctgat ttggatggag
      181 gaaaaaataa agctcaattg atcattgatg atatagtggc gtatcttaaa aatccttcag
      241 tttattcgct tgaaaaagaa ggtccgttaa atcattttgt taataattgt agcgagattg
      301 aaccatgggt tttaatcggt ggtggactta ttattattgt ctccacatct gagcaagacc
      361 ctgaaggtct gattgtaact gtgggggata ttgaaggaaa gtgtgctaca cagattcccg
      421 taccacctgc gcttacaaca tccctcaaag atatttttat tcgccctcat acgaacgcaa
      481 agtatgagag ttttacaaaa gagcagataa aagccaacat taaatctaat gttataaatt
      541 ataattgtaa attgcatttt aatgaaaaac cagaaaatga gatcgtgtgc aggcatcttt
      601 ctctacaata ttgtatagat tcaatgaatg aacatacagg gaaagttcca ttaaaggctt
      661 actattcatc tccggaagat atacaaaaac atattccatt cgagcttgag cagcaatttg
      721 acaatctaca aaaaaatcca ccacccggta catgcgttgt tgcctgtgat aagtttgggg
      781 aggcattatc agtctttttt cacagaatgg aaaaagagaa gttaacgcat atggcggcaa
      841 tcgttcaatc tcaaacacac gctatggccg tccgcttgag aatcaaaaaa acgcctgctg
      901 gtgaaacaga atatgttgta tccttttatg atcctaatgc aaccaatact gcagtacgct
      961 ataaagcaaa caactgtgat tcttttgggt cattgcgatc ctttatgaat attaaacagg
     1021 cacaaaaaaa atgggtaatc acagaaattt gctccgactg tgtaggaata actccttatc
     1081 tccctcgaga acaagcccat ttattaagtg gtataaataa tgagttgcaa ccaccattat
     1141 caccatcagc attacatcta ttaatacaaa tggggatgca tgaaaacatt gttctttttt
     1201 ttgataaatt taaaaactcc caagaaatga cagcatcaaa ggttcttgct gcgaaatc
//
LOCUS       DAZGCQ010000319         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000319 DAZGCQ010000000
VERSION     DAZGCQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.325"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..147)
                     /locus_tag="ACHK07_005360"
     CDS             complement(<1..147)
                     /locus_tag="ACHK07_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO8105524.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLES"
     gene            complement(320..550)
                     /gene="ynfN"
                     /locus_tag="ACHK07_005361"
     CDS             complement(320..550)
                     /gene="ynfN"
                     /locus_tag="ACHK07_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO8105525.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(582..620)
                     /locus_tag="ACHK07_005362"
                     /pseudo
     CDS             complement(582..620)
                     /locus_tag="ACHK07_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(623..811)
                     /gene="ynfQ"
                     /locus_tag="ACHK07_005363"
     CDS             complement(623..811)
                     /gene="ynfQ"
                     /locus_tag="ACHK07_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO8105526.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            complement(822..1034)
                     /gene="cspI"
                     /locus_tag="ACHK07_005364"
     CDS             complement(822..1034)
                     /gene="cspI"
                     /locus_tag="ACHK07_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO8105527.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSVIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          412 a          246 c          203 g          391 t
ORIGIN      
        1 gctttcaaga ccagtcattt tttcccgtgc agtaaactgg atactggtaa cttctttccc
       61 ggtctttttc ttaagctcaa tgattttttt tgttatatat tcagaaatat ctgcttctgt
      121 ttttgttttt aactcttcaa tgttcatcat ttcctctttt agtctgttat gactttcctg
      181 gtacacagta gtgtcaatta tatggagcaa acgtataaaa gataagatga aacatcgcaa
      241 taatcaacat acgacggtct aaatttcaca caaacagata aagatgatta tcatttatta
      301 tcaatgcatt agaatcaaat caattcaaga gtctcattgc tgcttccaga atttcttctg
      361 aagttacatg tcgatccgct gctacataaa tgactttatg atctccggtc agagatggaa
      421 accctgcagc cattacagtg aggtgtgttg tttcgccatt tggatattca cgcatgatgg
      481 tgttaactcc ggtcatcact ggcactacca ttgctggttc agagttaaaa aaaactatta
      541 tttttttcat aatgttaccg taatatgtga gtatccatcg actagacact aagcaaaaaa
      601 gctcccgaag gagccttcat tttcactttc ttaaatctaa cgacagacgg ctaacattta
      661 agtattgtga aatattatca aatgtaatca tcattgattt acaaaagata cattttgccc
      721 cgaaaggatt cctgtcagaa acatcaaaag atgatgttct atactgagaa ccatggcaac
      781 acgggcatct aaagtgaata tggtttgtaa tattttctac ctcaaagtgc cactacatga
      841 acagccgcgg ggcctttagg tccgttctca ataccaaatt cgacttcctg attctcagtt
      901 aacgttttga aatcgttgct ctgaataact gagaaatgga caaacacatc tttgctacca
      961 tctttcggcg tgatgaaacc aaaacctttt tcagggttaa accatttcac taaaccagtc
     1021 attttgttag acatcattat taccttttga agaagttagc ccttgggcag aatggtccga
     1081 aaaaaaatat cagagagaaa aaccaacaag gagatctcaa gaggtacaaa taataaaatt
     1141 ataacaatga ctgcttcaga taattttgta acaaaccaga acaccattaa cgcatgatta
     1201 accaaccata gcaaggatta cttttgtaaa gaaaaacaca cgaatgaaac aa
//
LOCUS       DAZGCQ010000320         1234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000320 DAZGCQ010000000
VERSION     DAZGCQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.326"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..1061
                     /locus_tag="ACHK07_005365"
     CDS             30..1061
                     /locus_tag="ACHK07_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105528.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDNPDRFNINNSSVTYINIGSNDSDYITIGIDNQEPIKLLAS
                     YKDKELARTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENKKVGQTIINENIVENTHDINNKKMIFSAIKEIYDEDPSFIFDKISHKLRHT
                     VTEFDESGKSEPTGLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFR
                     IDGDNLTQYCSKKARSASAWFEHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNP
                     SNLARGYEY"
BASE COUNT          497 a          203 c          210 g          324 t
ORIGIN      
        1 taacaaatca atcaaaatgg aataaaatca tgctaccatc tatttcaatc aacaatacca
       61 gtgcagctta cccagaatcc atcaatgaaa ataacaatga tgaagttaat ggattagtac
      121 aagagttcaa aaaccttttt aatggtaagg aaggaataag cacctgtatt aaacatctgc
      181 ttgagcttat aaaaaacgcc atacgagtaa acgacaatcc tgatagattt aatattaata
      241 attcctcagt tacttatatt aatattggtt ccaatgattc agactatatt actattggta
      301 tcgataacca agaaccaata aaattacttg cgagctataa agacaaagaa ctcgcccgta
      361 ctatcattaa tgacaacata gttgagaaga ctcatgatga tataaataat gctgaattta
      421 ctgatggtgg tattgatgat cgtagtgata ttggcattga ctccaatgat acagacgata
      481 ttgatattga taatcaagaa ccaataaaat cacccgcgaa cgatgaaaac aaaaaagtcg
      541 gccaaaccat catcaatgaa aacatagtag agaatactca tgatatcaat aacaagaaaa
      601 tgatcttcag cgcaataaaa gaaatatatg atgaagatcc aagttttatc ttcgataaaa
      661 tatcacataa actcagacat acggtaacgg aattcgatga gagcgggaaa agcgaaccaa
      721 cgggcttatt tacctggtac ggtaaagata aaaaaggcga ctctctcgct attgtaatta
      781 aaaataaaaa tggaaatgat tacttatctc tcggttacta cgatcaggat ggctaccaca
      841 ttcaaaaagg atttcgtatt gatggtgata atctcaccca atattgtagt aaaaaagcca
      901 ggagtgcttc tgcgtggttt gaacacagta aagcaatcat tgcagaatca tttgcaacag
      961 gttccgatca tcaagttgta aacgaactca aaggggaaag actgagagaa cttaacgaag
     1021 cttttaatcc ttcaaatcta gcaagaggat atgaatatta agaggatgat agaaaatata
     1081 cacttgagaa tacaaaagaa atagtttcta ctttattcgg taacgaagtt aacgttgacc
     1141 attctcaaaa aatatataag cactttaagg aactcgaagg taacattgaa aaaggcttca
     1201 agatcgccag acgaaatatc aaaatgaaat taat
//
LOCUS       DAZGCQ010000321         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000321 DAZGCQ010000000
VERSION     DAZGCQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.327"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..>1219
                     /locus_tag="ACHK07_005366"
     CDS             169..>1219
                     /locus_tag="ACHK07_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO8105529.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RNVNSLNVGEKLNDDNLAYHSIFTNEFSVIIPPSYYDATSVVNANNGVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLDSLVDESFNENTDNIQTVKDLLTQLTDGM
                     ELFALRDIVAFPSSIIAKLLKSTLNSDHELVMRALDTYLCYFRNKDLSNDTEIINFFH
                     ALFLKKPELMVSENYR"
BASE COUNT          412 a          193 c          239 g          375 t
ORIGIN      
        1 tagaaagatt gatcgccagt ctgtttagag cggtgcagca gcaaactttc ataaccaggt
       61 taatcctgag tttgcagtac gaaaagttaa aaatgagcga tttctgtaag attccccatt
      121 ttgccaggct tatgattcgc agatcattca agtgcaaagg agcgatgtat ggattgttca
      181 aaatgcaatg gttatgcgac gatgttatta aatatggtgc agggttctga cccggttaat
      241 ttactggaat tacatggttt ccttgaacat ttcgcatatt atgtttcatt tggcaaattt
      301 aatgctggcc accagcgcta taatgcattt aaaaaattcg tcagtgagat ttcggagatc
      361 agcgcaaacg atatcaatat gacgattaaa accgggcaat ccagacatga aaatgttatc
      421 tctataaata tgaatgatgc aattcctcgt gatgagaaag ggatcaccgt cagaatagat
      481 aatattaatg gaaagaaaaa taactctaat tcatcagatg tttttattcc ttatgtaaat
      541 acatttcccg acctcaaaaa taaaatactc aggatgaaaa ttgaactgac tgaaggtagt
      601 ggtttttcta aaagtctttc tgacagtcag attgagatgc atattctgag gaatgttaat
      661 tcgctaaacg ttggcgagaa attgaatgat gataatcttg catatcattc tatattcact
      721 aacgaattct ctgttattat tccgccatcc tattatgatg cgacttctgt agtaaacgcc
      781 aataatggtg tgagagaaaa actatttgaa agtgatagta aagttaaaga tatcgtcgat
      841 gatatgtcta atcatgatgt ggagagcgaa aaagatatat ttgttatcgg aggaatgata
      901 gaaaaactgg attctctggt agacgaatct ttcaatgaaa atactgataa catacaaaca
      961 gtaaaagatt tgttaacgca attaactgat ggcatggaac tcttcgcgct gcgggacata
     1021 gtggcattcc catcgtccat aatagcaaaa ttattaaaat ccacacttaa tagcgatcat
     1081 gaacttgtta tgcgcgcatt ggatacgtat ctgtgctact tcagaaataa agatctgagt
     1141 aatgatacgg aaataattaa ctttttccat gctctctttt taaagaaacc agagttgatg
     1201 gtttctgaaa attatagat
//
LOCUS       DAZGCQ010000322         1217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000322 DAZGCQ010000000
VERSION     DAZGCQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.328"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..1156)
                     /locus_tag="ACHK07_005367"
     CDS             complement(5..1156)
                     /locus_tag="ACHK07_005367"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO8105530.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          272 a          288 c          267 g          390 t
ORIGIN      
        1 agattcaatc tgtcaatgca acaccccttt caattatctc tttcggtgtt ttgaacttca
       61 gtgtctttct cggtctgttg tttagctgag cagcaaccag atctagttca tgttgagtat
      121 attgggcaag acatgtcttt ttaggaaagt actgccgaat tagcccattt gtgttctcat
      181 ttgttccccg ctgccaagga ctctgaggat cgcagaagta aactttaacg ccggtgctga
      241 cagtaaattc tagatgtctg gccagttcca ttcctctgtc ccatgtcagt gattttctga
      301 gttctgacgg taaactcagg aatttgtcgg taagagcctg atttactgag acagaatctt
      361 tgcccctgag tctaaggatg atcgtataac gtgattttcg gtctacaagt gtggctatat
      421 gagagttttt tgtacctgag actaaatcgc cctcccaatg ccccagagag cgtctgttat
      481 cgatatttcg ggaacgttcg tgaattggtg ttccgttcac tatgttaatc gtacctcttt
      541 cgcctttgcg ggtatgacgc ctgccatggc gaaggctatg cgaccgtcgc agatgctgta
      601 tattcaggtg gtgtagcgct tcacggctac gaaagtacag cgttttataa attgtctcag
      661 gtgatattcg cagcgttttt tgacgtggtt ttgttcgcct taaccatcct gatatttgct
      721 ctggagacca tttcatctcc agcttttcca gaacaagctt tcgcaatggt aaattttgat
      781 ccagtaagca cggttttggc cttttcgcca ttctgttggc tcggttatta gcatcaacag
      841 ctttgtaata gcgtctgccc cgattacgct gaacttcacg tgagatcgtc gaaggactgc
      901 gattcagcgc agtagctatc gcacgaatgc tcattttggc tgacaaacca gctcgtatct
      961 cctcgcgctc agacagtgtc aggtgagcta cagcccgctt acgctcatgg ggttttatgc
     1021 cgccagtatc ccttaacata gtgaagatcg ttccgggttt tgaacccagg atattcgcta
     1081 tttcactgaa gcctgttccg ttcttccata gttcaaaaac agaggctttt tcctctgctg
     1141 taaatgttcg tctcattcaa aaaacctccg caaccccatg ttttcacata actgttgcgt
     1201 tgaccaattg aatctac
//
LOCUS       DAZGCQ010000323         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000323 DAZGCQ010000000
VERSION     DAZGCQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.329"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..665
                     /locus_tag="ACHK07_005368"
     CDS             222..665
                     /locus_tag="ACHK07_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8105531.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            681..968
                     /locus_tag="ACHK07_005369"
     CDS             681..968
                     /locus_tag="ACHK07_005369"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8105532.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            981..>1213
                     /locus_tag="ACHK07_005370"
     CDS             981..>1213
                     /locus_tag="ACHK07_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO8105533.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGV"
BASE COUNT          328 a          257 c          301 g          327 t
ORIGIN      
        1 accattactc tttccagttt ttgcccacgg tagcaaatat cctgttgcat tgtatatgca
       61 ggaaccgaat atagccaaag catggcggca gagaaatgat agcgctatca caaagatcgg
      121 tagcgttatc agatagcgct atcaaatgcg acaatcatca cattaattaa tgcgtaaaat
      181 atctactctc ctgttaatta tcatcgggag ttatcagtcg catgttaaaa aatctattaa
      241 atacaaaggt cgttcaggtc gtggaacagg tcaaagactg gcgggaagcc gtggcgattt
      301 catgccgacc gttgattgaa aacggttcta tcgaaccacg ttatgttgat gcgatctatc
      361 actctcacga cactatcggt ccgtactatg tggttggccc tggtattgcg atgcctcatg
      421 cgcgcccgga agagggggct aacaagctgt cattagcact gaccctgatc ccttcaggtg
      481 tgaattttga tgccgatgaa aacgatccag tgaaattact gattgtgctg gctgcaaccg
      541 acagtaccag ccacattgaa gcgatttctc aactcgccaa gttattcgat aacgaaaaag
      601 acatacaggc tattttgacg gccaaaacga cgcaggacat tttgtctgtt attgcgcgct
      661 attaatcctg aggaacattt atgaaaatta cagtggtttg cggtaacggc ttaggcacca
      721 gcctgatgat ggaaatgagc attaaaagca ttttgaagga tttgtccgtc agcgctgatg
      781 ttgatcatgt cgacctgggc tccgcgaaag ggacgccgag tgatatctat gtcggcacca
      841 aagacattgc cgagcagtta gtcgcgcagt ctgtcgcagg caaaatcgtc gcactggaaa
      901 atatgattga taagaaagcc atgagagaac gtttatccgt ggcattaacc gaattaggcg
      961 cactgtaagc tgggggcgat atggatttct ttcgtttttt gatgagcgat gtactttccg
     1021 aacccgcagt gctggtcggt ttaatcgccc tgattggcct gattgcacag aaaaaaccgg
     1081 taaccgaatg cattaaaggt accgtcaaga ccattatggg gtttgtgatt ttaggcgcag
     1141 gggcgggttt agttgtgtcg tcactgagtg acttcgcaaa cattttccag tatgcctttg
     1201 gtattcaggg tgt
//
LOCUS       DAZGCQ010000324         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000324 DAZGCQ010000000
VERSION     DAZGCQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.330"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..199
                     /locus_tag="ACHK07_005371"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHK07_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            281..877
                     /locus_tag="ACHK07_005372"
     CDS             281..877
                     /locus_tag="ACHK07_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO8105534.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            880..>1207
                     /locus_tag="ACHK07_005373"
     CDS             880..>1207
                     /locus_tag="ACHK07_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105535.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARV"
BASE COUNT          480 a          196 c          209 g          322 t
ORIGIN      
        1 ctcttcaaca tcagacagtg aaagcaaaaa tacaagcact gacagtacgt tatttaatga
       61 aaaattccca tttcaactaa gacaagattt cgcatcaaga ataacagaag atgaaagtga
      121 tttttttaaa tggaaaaaaa taagagagtt ggcatttgtg agcaatcata aagaatggaa
      181 taaatatgac ttattaattt taaaatctgt taatgaaatc aacattcatt tgtccagcac
      241 tccatacttt cagccgttgg attggtatat aataaaagcc atgctctgga ctgaaaacga
      301 tgcggaaaat acttctcagt ggaatggtta tccattacaa attggtcgtt ttcggaaaga
      361 taaagcgatg ccagctttaa tctctggaga aaaatctaca gcccttgtta ccccaccaca
      421 atggagaaac aaagcattca atgggttaaa agatccagaa agaaattact gggcaaaaga
      481 acaaataact ggctctccag aagagaatat caaagcagca attacttact taatgatgaa
      541 actttctaat acaaaggaag aatcaactat cgatcaatat gactcaactc tatatagcgc
      601 cattgtccaa aaaggcgatt tggctgacaa catcagaaaa gagagaaaaa cgaccatacc
      661 taacttaacg aaaaacaatc ctggaaaaaa tctcgataaa atacacccgg gagatatttt
      721 atattatcaa aaagcatcca tgaaagtaat aattactgga tggaaaccta tcacaataaa
      781 aaatgtagcg atgaattata atggtggtgg cgatcccaag tatgcaataa aacttcagtt
      841 cgtttataca ttactcacaa aaaacagagt attatgagaa tgcgaaaaat aacaattatt
      901 ctactgctga cattctcatg tacaaaaggt ctggcatttg acatttccat gtctaaagaa
      961 tgtagcggtg ttgatgaact tgaatttatt gattgtgtta aaaactcatt tattatgagc
     1021 actattacac ttaaaaaagc agaagcaaat ataaaagaga aaattcaaaa atggaatagt
     1081 gatgaggacg aagcaataag aaaagaagca gttatttctt taaaaagact agagtcttct
     1141 aatagggaat ttatacgtta cagatttgct caatgctctt ttactctaac ctggggggcc
     1201 agagtag
//
LOCUS       DAZGCQ010000325         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000325 DAZGCQ010000000
VERSION     DAZGCQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.331"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1203)
                     /gene="espI"
                     /locus_tag="ACHK07_005374"
     CDS             complement(<1..>1203)
                     /gene="espI"
                     /locus_tag="ACHK07_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO8105536.1"
                     /translation="DNNGNKVGVLDVPMLDFSSLSRDGHTTLIHPGYVVSAKHGGLQS
                     VSSATFGYDQIYKMVDNNLAGIDFSAPRLNKLVTEVIPADIQGKDKFNNNRYTAFYRA
                     GVGSQYIRYANGTDKLLQAYTPEKAYLTGGTVGKPYYTHYNGMKMISANPGNTFDKNQ
                     GPLASYGQSGDSGSPLYAWDNIDKKWVLAGVTLHNYGVKGARNDWLLIPHDFISQKLQ
                     DDLKPIIVASPEENILRWEFDRSRGTGTLSQGEKIFSMTGSVNGNANTGNNLVFSGNE
                     GKIELVSSVEQGAGYLQFDKDYTVLTNNNSTWTGAGIIVGDEANVKWGVNGIAGDNLH
                     KVGSGTLTVNGHGENKGGLKVGDGVVVLEQQPDANQKQQAFSHINIASGRATVKLNGA
                     NQVDADNIS"
BASE COUNT          331 a          291 c          214 g          367 t
ORIGIN      
        1 gctgatatta tctgcatcta cctggtttgc cccgttaagt ttaactgttg cccgaccact
       61 ggctatattg atatggctaa acgcctgttg tttctggttt gcatctggct gttgctcaag
      121 aacaacaacg ccgtccccaa ctttaaggcc acctttattc tcaccatgac cattaacagt
      181 taatgtcccg gaaccaactt tatgcagatt atcaccggca atgccattaa ctccccattt
      241 gacatttgcc tcgtcaccga caataattcc ggcaccagtc catgtactgt tattatttgt
      301 cagtacagtg tagtctttat caaactgaag atatccggct ccctgctcca cactggatac
      361 cagttcgatt ttcccttcat tacctgagaa cacaagattg ttcccggtat ttgcatttcc
      421 gtttacacta ccagtcatgg aaaaaatctt ctctccctga ctgagagtac ctgtacctct
      481 cgaacgatca aattcccagc gtaagatatt ctcctccgga gaagcaacaa taattggttt
      541 aaggtcatcc tgtaattttt gactgataaa gtcatgaggt atcaggagcc agtcatttcg
      601 tgcacctttt actccataat tatgcagagt aactccagct aatacccatt ttttgtcaat
      661 gttatcccag gcatataacg gcgaaccact gtctccactc tgtccataac tggcaagagg
      721 tccctggttt ttatcaaagg tatttcccgg gtttgctgaa atcatcttca taccgttata
      781 gtgagtataa taaggtttcc ccactgttcc gccggtcaga taagcctttt ctggagtgta
      841 agcctgcagt agtttatctg tgccatttgc ataacggata tattgagagc caacgcccgc
      901 acggtaaaaa gccgtatacc ggttattatt aaatttatcc tttccctgta tatctgcggg
      961 aattacttct gtaacaagct tatttaatcg tggggcagaa aaatctatac cagcaaggtt
     1021 attatcaacc attttatata tctggtcata accaaaagtt gctgatgaaa cactttgtaa
     1081 accaccatgt ttagccgata caacataacc aggatgaatc aatgtggtat gaccatcacg
     1141 agaaagactg gaaaaatcaa gcatgggaac atcaagaacc ccaactttat ttccattgtt
     1201 gtc
//
LOCUS       DAZGCQ010000326         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000326 DAZGCQ010000000
VERSION     DAZGCQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.332"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1049
                     /locus_tag="ACHK07_005375"
     CDS             <1..1049
                     /locus_tag="ACHK07_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8105537.1"
                     /translation="DGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSE
                     FVAWLKEKGAFDQPYWMMKASLPASMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRV
                     TIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLS
                     SDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGE
                     TGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSRVMGFRLGNPVT
                     YELQEGDHGWSVNLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGSHWRTVGVGE
                     YVSSGAPGREYFSL"
     gene            1091..>1186
                     /locus_tag="ACHK07_005376"
     CDS             1091..>1186
                     /locus_tag="ACHK07_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105538.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYE"
BASE COUNT          312 a          250 c          405 g          219 t
ORIGIN      
        1 cggacggatg cctggagaaa gcgaaaaacg gtgacgatat cccggataag gtgaagtttc
       61 tgaacaccgt gggggcagcc agagtatacg ggcgggacat tcatacgggg gccggtgaat
      121 ggaccacgag tgagtttgtc gcctggctga aagaaaaagg ggcatttgac cagccttact
      181 ggatgatgaa ggcatcactg cctgcgtcaa tgaataaagt catcacggat gtcggaccgg
      241 gaaaactcaa tctgggtggc tgtgtcattg aggtgatggg aacgtatgag gctgccatag
      301 tccgggtcac cattggcgaa tacggtgcaa cggggtttat taatggcacg gtctgtacct
      361 gtacggttta cggcgacaca cagtatttcc actggcgggt ggattacagc acaaaaaaca
      421 aaccggatac ggtcagccag cgggatgcca gcacgacgca gaaaggtgtg gtgcagttaa
      481 gcagtgatac tggcagtaat gacgaaacaa aggcagccac gccgaaggcc gtgaaggcgg
      541 caatggatgt ggcaaatgaa gcgaaaacaa aggcagaaga ggctgcagca ggaggtggtg
      601 ttcccggtcc gaaaggagag aaaggtgacc ccggagcaca gggtccgaaa ggtgaaacgg
      661 gagcaacagg ccccgctggt ccgcggggag cgcagggacc gaaaggagag aaaggagacc
      721 ccggtcccag aggtgagcgt ggagaaaccg ggccgatggg gccgccgggg gcatcaggag
      781 atgacgggaa gagtcgtgtc atgggcttca ggctggggaa tccggtaacg tatgagttgc
      841 aggaggggga ccatggctgg tcagttaatc tggctgatgg ttcagtcatt acagggcttt
      901 cggggaacac tcagggcggc gtcagccgtg ttacaacgat ttactacagg ccgctgcaga
      961 tgacgtttga tggctctcac tggcgcaccg ttggtgttgg tgagtatgtg tcgtcaggag
     1021 cgccggggcg tgagtatttt tcattataaa gcgcatcatc tttcagacat gtaaccggga
     1081 ggacttacag atgcacataa agaatttcag acaatacacg ccggaaaatc cggatgtgcc
     1141 gggtgcgatg tacctgaaat cagaggatgg tcaggactgg tatgag
//
LOCUS       DAZGCQ010000327         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000327 DAZGCQ010000000
VERSION     DAZGCQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.333"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..226
                     /locus_tag="ACHK07_005377"
                     /pseudo
     CDS             1..226
                     /locus_tag="ACHK07_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(993..1175)
                     /locus_tag="ACHK07_005378"
                     /pseudo
     CDS             complement(993..1175)
                     /locus_tag="ACHK07_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          333 a          262 c          219 g          361 t
ORIGIN      
        1 tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg
       61 taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg
      121 tcagcaccgg gccagcaacg aactgcgtaa acagggggtt tttatctccg acagtggtgt
      181 ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt
      241 tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc
      301 agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt
      361 gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta
      421 atccggaagg attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt
      481 aatgcatggc tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct
      541 atgtaaactc tctgactgtt cattttattt gttgtttcag ggtcggaact caccctcaat
      601 aaacaactga atacgagtag atattcatcg tctgagctat atggctttac acaatagccg
      661 acatgtcttt tctcagtcag gaattttact tttcctcact acgaagttga cgacttcgat
      721 atgggataga ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca
      781 atatttgtta taaaataatg cgtaacatta atttcaccaa caaaaaaaag atttacgcta
      841 tccccttgcc ctggtatttt accatattta caccacactt tttttgtatc tggatcattc
      901 tttgatacga ttttcgccac ttgttccctg gccctcaccg tcaatgtttt cttaaacctc
      961 cccccatcat cgattgttag ggaaaaataa gaaaaaaaat aatcatcttc tacatttgtg
     1021 gatattgata tatccaccgc tacgtgcgga aaaaaaatct gggagccatg taaaaactcc
     1081 atcattttct ggtattgcca tatagtttat gtaatgctta cttacaagag attcatcttt
     1141 gtgtaaataa agataagtag tcacgcataa aaaaa
//
LOCUS       DAZGCQ010000328         1171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000328 DAZGCQ010000000
VERSION     DAZGCQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.334"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..407)
                     /locus_tag="ACHK07_005379"
     CDS             complement(144..407)
                     /locus_tag="ACHK07_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8105539.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            597..836
                     /locus_tag="ACHK07_005380"
     CDS             597..836
                     /locus_tag="ACHK07_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105540.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(887..>1171)
                     /locus_tag="ACHK07_005381"
     CDS             complement(887..>1171)
                     /locus_tag="ACHK07_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO8105541.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
BASE COUNT          337 a          298 c          226 g          310 t
ORIGIN      
        1 tggcatccac atagtgcgct gggttatcgc tctccacggg aatatctgcg gcggcgggca
       61 gtaatgggtt aagtgatacc agatgtctgg aaatatagga gcaaatccac cacgcatgga
      121 gcctgttaag actctcactc tcatcattct cccttctgag tcccaacgaa acgaccaaaa
      181 cgctgagtaa agcgggcaac attgccttct gatgccaccg ttctgagttt accggtatag
      241 aacggatgcg atttggagga aacgtcaatc gtcacatacg gatacgtcac accatccagt
      301 tcaatctcgc gatctgtttt gatggtagaa cctattttaa agtactcatc cacactcgtg
      361 tcatggaaca ctactgtgcg gtattcagga tggatatttg gcttcatctc tcttcactca
      421 tgttataaca taacaataag atacattctc agctagatca tttcaacaga atccccgagc
      481 ccaagggtaa tgtggcatcg cagcctctca tcaaatggaa gacatgtgat tgcacattta
      541 tcatacaaga cgtatgataa cactgtgtta tttatcctgc tgctgagggt atagatatga
      601 ataacgaatc tgaaaaagca atgtggcgtt gtaagcctgt ggataatcca tggaccttca
      661 cttccctgct gaaaaaagca gtctgtgccg ttttaccaga tttcacacct gctcaagata
      721 aaccccaaaa tatgttttca ctaagtaatg aaaaagatac tgattattca gtaccaatga
      781 gaccctttgc attatatgac ccgtggaaac atcaccatga caatgctgat ttctaagaaa
      841 agcaagggtt tccccaatta tcccagctaa ggaaacccta agaattttac ttattagcta
      901 aatctccact ttttgctgct gccttagata cattacttgt tcctttgtta attgcatccc
      961 ccataaccgt tccgacactt accccagccc aggacaacgc tcccagccac actgccggca
     1021 agacgagaaa catcgtcccc atcaccagat tcattatcag atcatccgat gtgttctgga
     1081 atcccatcat attgaaccgg gtatgtgtat ccgaactgta cagcgccgtc agcatccagc
     1141 tgtcaagcca ccgtgcaagc tcccaccaga a
//
LOCUS       DAZGCQ010000329         1165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000329 DAZGCQ010000000
VERSION     DAZGCQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.335"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..684
                     /locus_tag="ACHK07_005382"
     CDS             235..684
                     /locus_tag="ACHK07_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105542.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQNQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            876..>1165
                     /locus_tag="ACHK07_005383"
     CDS             876..>1165
                     /locus_tag="ACHK07_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8105543.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTLLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDF"
BASE COUNT          294 a          249 c          337 g          285 t
ORIGIN      
        1 tagtgtgttt ctgtgacggg aataaaaaaa tacagcacag ccgatgaccc ggtgctgaaa
       61 acaggttaaa tgtagtgggc acatttccgg taatatcagg aacagcatgt tatgcttata
      121 aaagcatata ccgggagtgt gccctgacac tgtgatgttc tggttgtctt ttgaccattt
      181 tccggggacg aaatataagc tgttctgcgt taacacttaa taatgagaga ttgtatgctg
      241 aaaaaaacaa ttctggcgtt atgtatttcc gctccatttc tggttgcggg ctgtgatgat
      301 gcgacaaagg agaatgtaag taagaaggcg gcagatataa aggaagctgc gcagaatcaa
      361 ctcagtgatg tgaaggatgc cgcggaggac gtgaaaatgc aggctgccgg aaaagtggca
      421 gaggtttctg aagcggctca gtccgggctc agcgaggtga aggatgccgt ggaggaagtg
      481 aaaacacagg cagccggaaa gatggcagag gtctctgaag cggctcagtc cgggctcagc
      541 gaggtgaagg atgccgtgga ggaagtaaaa acacaggcag ccggaaaggt ggcagaggtc
      601 tctgaagcga ctcagtccgg tctcagtgat gtgaaggata ccgctcctga aaagggggct
      661 gaaatatccg taacggagga gtaaaagagc gccgccggtg cagttttttt cagcacccgg
      721 tgacacggaa ttttatatac ggggcatgtc tgtctcttta ataaatcagc atatttaccg
      781 gatatccgat gccctgtacc tgcgctgtca ttatggctct caaacagaca atgctttgtc
      841 tggagcacag gaactctgtc atacctgccg gcacaatgct tctgattgcc gggcatctgc
      901 gccctgcttt tcaggctcat accggacgtt tccttatcgc tgacctcagt gaggaggtgg
      961 tgaggcggta tctgcggttc tgcacgctat tatccggtat ggcagtcagc ccgtcgtctt
     1021 tgcttttaca gatccggcca cagatgtccg tgctggcgga atgtctgatc agacaactta
     1081 ttacagatga accatcgccg gtgtacttca gggatgcggt ggctttttcc agtctggcgc
     1141 tgtttgagga acatccggat ttcca
//
LOCUS       DAZGCQ010000330         1154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000330 DAZGCQ010000000
VERSION     DAZGCQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.336"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..729
                     /locus_tag="ACHK07_005384"
     CDS             <1..729
                     /locus_tag="ACHK07_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8105544.1"
                     /translation="LNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     EQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
     gene            781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHK07_005385"
     CDS             781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHK07_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8105545.1"
                     /translation="MATKEENLNRLRQLAGLLGREVDMSGSAADIALRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEQLNNTDSPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTA"
BASE COUNT          262 a          258 c          375 g          259 t
ORIGIN      
        1 ctgaacgacc cggcctaccg tcgtctgcgt atcatcactg ataacctcaa acaggaagag
       61 catgccattg tccaggtgga ggaaatgcag gcggtgaatg ccgtgctgta tggcaaatac
      121 accatggaag gggagcagtt tgatactgtc gaggtggatt tcgggcgctc tgaaggaaat
      181 aacattgagc aggctgacgg taaaaaatgg tctgagcagg accgtgatac gtttgatccg
      241 acgcatgata ttgacctcta ctgcgatcag gccagcggtc ttgtgaatat cgccattatg
      301 gacggtacgg tctggcgtct gctgaatggc tttaagttgt tccgcgaaaa actggatacc
      361 cgtcgcggct caaattcaca actcgaaacg gcagtgaaag atctgggcgc agtggtgtcc
      421 ttcaaggggt attacggcga tctggccatt gtggtggcga aaacgtctta tgtggcagag
      481 gacggtaccg aaaaacgtta tctgccggag ggcacgctgg tcctgggaaa tacgtcagcg
      541 gagggcatcc gttgctatgg tgccattcag gatgcgcagg cgttgtccga aggtgtggtg
      601 gcctcttccc gttatccgaa acactggatg accgtgggcg atccggcccg tgaattcacc
      661 atgacgcagt ccgcgccgct gatggtgctg ccggatccgg atgagtttgt ggtggtacag
      721 gtgaaataat ccgggagcgg gggcgaaatg cccccgtgtc ttttttcaca gggggctgag
      781 atggcaacaa aagaagaaaa tctgaatcgt cttcgtcaac tggctggtct gctggggcgc
      841 gaggtggata tgtcggggag tgctgcggat attgctctgc gtgtgtctga gtgggaagag
      901 gagcttgcag cttcccggga gggcattatg cactctgatg agagcgggtc tgatcaaaat
      961 gacacagacg atggtgagca gttgaacaac acggattctc cggatgatgt taaagccgtc
     1021 cgggtacgga agtgcctgca agtaatgggg tattgcccgg agacaggtcg tcccgttgag
     1081 ctggcgttac ggggtatgcg tgttctggtg ccatcatcac tggcaacggc catgatacag
     1141 cacggaacgg ctga
//
LOCUS       DAZGCQ010000331         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000331 DAZGCQ010000000
VERSION     DAZGCQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.337"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1148
                     /locus_tag="ACHK07_005386"
     rRNA            <1..1148
                     /locus_tag="ACHK07_005386"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          279 a          263 c          357 g          254 t
ORIGIN      
        1 ataccagctg gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac
       61 gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt gatggggtca gcgcaagcga
      121 agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga
      181 aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc
      241 cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg
      301 gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg
      361 ataggtggga ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca
      421 ccctttaatg tttgatgttc taacgttgac ccgtgatccg ggttgcggac agtgtctggt
      481 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
      541 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      601 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      661 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      721 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      781 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      841 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      901 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      961 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga
     1021 gatgagttct ccctgactcc ttgagagtcc tgaaggaacg ttgaagacga cgacgttgat
     1081 aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgaggc
     1141 ttaaccttac aac
//
LOCUS       DAZGCQ010000332         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000332 DAZGCQ010000000
VERSION     DAZGCQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.338"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1028
                     /locus_tag="ACHK07_005387"
     CDS             48..1028
                     /locus_tag="ACHK07_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFO8105546.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
BASE COUNT          321 a          304 c          307 g          221 t
ORIGIN      
        1 gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac
       61 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
      121 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
      181 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
      241 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
      301 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
      781 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac
      901 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
      961 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DAZGCQ010000333         1152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000333 DAZGCQ010000000
VERSION     DAZGCQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.339"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..1115
                     /locus_tag="ACHK07_005388"
     CDS             75..1115
                     /locus_tag="ACHK07_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HFO8105547.1"
                     /translation="MLHTTNPVIKHKAGLLNLAEELSNVSKACKIMGVSRDTFYRYRE
                     LVDEGGVDALINRSRRAPNLKNRTDEATEQAVVDYAVAFPAHGQHRTSNELRKQGVFI
                     SGSGVRSVWLRHNLENFKKRLKALEEKVARDGIELTDSQIAALERKASDDEACGEIET
                     AHPGYLGSQDTFYVGNLKGVGRIYQQTFVDTYSKVAHCKLYVTKTPITAADLLNDRVL
                     PFYASQGLPMLRILTDRGTEYCGKVEQHDYQLYLAINDIEHTKTKAMSPQTNGICERF
                     HKTILNEFYQVTFRKKLYGDLDTLQSDLDEWLAHYNNERTHQGKMCNGRTPMETLLDG
                     KRIWAEKNLSQM"
BASE COUNT          331 a          263 c          288 g          270 t
ORIGIN      
        1 gagccttgaa aggttgagag ttaccggttt tgatatgggt gtcgaatcct tatacaaaac
       61 acgaggtaac tctcatgctt catactacca atcccgttat caaacacaaa gccggtttgc
      121 tcaatctggc tgaagaactc agcaacgtat caaaagcctg taaaatcatg ggcgtttcgc
      181 gtgatacgtt ttatcgttac cgcgaactgg tcgatgaagg cggtgtggat gcgctgatta
      241 atcgtagtcg ccgcgctcct aaccttaaga accgtaccga tgaggcaact gaacaggctg
      301 ttgttgatta tgccgtcgct ttcccggcac acggtcagca ccggaccagc aacgagctgc
      361 gtaaacaggg cgtttttatc tccggcagtg gtgtccgttc tgtctggtta cgccataacc
      421 ttgagaattt caaaaaacgt ctgaaagcac tggaagaaaa agtagcccgt gatggcattg
      481 agctaactga cagccagatc gcagcactgg agcgtaaagc cagtgatgac gaggcctgtg
      541 gcgaaatcga aacggctcat ccgggatatc tgggttcgca ggacacgttc tatgtaggca
      601 acctgaaagg tgtcgggcgt atttatcagc agacgttcgt tgatacgtac tcgaaagtgg
      661 ctcactgtaa gctttacgtc accaaaacac cgattaccgc ggctgactta ctgaatgatc
      721 gtgtactgcc gttttatgcg tctcagggcc tgccgatgct gagaatactg accgacagag
      781 gcacagagta ctgtggcaaa gtagagcagc atgattatca gctttatctg gcaataaacg
      841 atatagaaca cacgaaaaca aaggcgatgt ccccgcagac caacggcatc tgcgagcggt
      901 tccataaaac aatcctgaac gaattttatc aggtaacgtt ccgtaaaaaa ctgtacggcg
      961 atcttgatac attacagtcg gatcttgatg aatggctggc tcactataat aatgagcgaa
     1021 cccatcaggg aaaaatgtgc aatggacgga cgccaatgga aacgttactt gatggaaagc
     1081 gcatctgggc cgagaaaaat ttaagccaga tgtaatctga cagatacctg tataaataac
     1141 cggtaactgt ca
//
LOCUS       DAZGCQ010000334         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000334 DAZGCQ010000000
VERSION     DAZGCQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.340"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHK07_005389"
     CDS             complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHK07_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8105548.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVYEQAYRNTGEDALGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAV"
     gene            complement(412..>1140)
                     /locus_tag="ACHK07_005390"
     CDS             complement(412..>1140)
                     /locus_tag="ACHK07_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8105549.1"
                     /translation="LNDPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     DQFEKIEVDFGRSTKNNIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGSVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          256 a          360 c          249 g          275 t
ORIGIN      
        1 acggccattc cactcctgac catgtctttt gccactgagg ggggcatcag aattactctg
       61 ccagcatcaa catgctcaac aggctcacct gtttcatcgt caacgccgca caggtaaaaa
      121 cacttcagaa gcctgatacg ttccagaata cctaaggcat cctcaccggt gttcctgtat
      181 gcctgctcat aaacggtatc atcaacatct gcgatttcat tttccgatgc gcaaacctcc
      241 tcttcccact ctgccacacg ctgtgcgata tccgctgcac tccccgacat atccgcctcg
      301 cgccccagca ggccagccag ttcacgaaga cgtttctgat tttcttcttt tgttgccata
      361 tccagccccc tgtgaaaaaa gacacggggg catttcgccc ccgctcacgg attatttcac
      421 ctgtaccacc acaaactcat ccggatccgg cagcaccatc agcggagcgg actgcgtcat
      481 ggtgaattca cgggccggat cgcccacggt catccagtgt ttcggataac gggaagaggc
      541 caccacacct tcggacaacg cctgcgcatc ctgaatggca ccatagcaac ggatgccctc
      601 agcagccgta tttcccagga ccagcgtgcc ctccggcaga taacgttttt cggtaccgtc
      661 ctctgccaca taagacgttt tcgccaccac aatggccaga tcgccgtaat accccttgaa
      721 agacaccacc gaccccaggt ctttcactgc cgtttcgagt tgtgaatttg agccgcgacg
      781 ggtatccagt ttttcgcgga acagcttaaa gccattcagc agacgccaga cggtaccgtc
      841 catgatggca atattcacaa gaccgctggc ctgatcacag aaaaggtcga tatcatatgt
      901 cgggtcgaac gtgtcacggt cctgttttga ccactcctta ccgctaccct gtatgatgtt
      961 attcttcgtc gatctgccaa aatcaacctc aattttctcg aactggtctc cttccatggt
     1021 gtatttgcca tacagcacgg cattcaccgc ctgcatttcc tccacctgga caatggcgtg
     1081 ctcttcctgt ttgaggttat cggtgatgat acgcagacga cggtaggtcg ggtcgttcag
//
LOCUS       DAZGCQ010000335         1139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000335 DAZGCQ010000000
VERSION     DAZGCQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.341"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..359
                     /locus_tag="ACHK07_005391"
     CDS             120..359
                     /locus_tag="ACHK07_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO8105550.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            379..711
                     /gene="sctI"
                     /locus_tag="ACHK07_005392"
     CDS             379..711
                     /gene="sctI"
                     /locus_tag="ACHK07_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO8105551.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            708..>1139
                     /gene="sctJ"
                     /locus_tag="ACHK07_005393"
     CDS             708..>1139
                     /gene="sctJ"
                     /locus_tag="ACHK07_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO8105552.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPI"
BASE COUNT          368 a          198 c          243 g          330 t
ORIGIN      
        1 atttaatgtg aatttgaaaa atcaggacac aaataatagt tcatttagct acggagataa
       61 ccgattcgag aagtctcaag gtagcaagtg gacatttcag gaataattga ggaggactta
      121 tggcagattg gaatggttat attatggata tcagcaaaca atttgatcag ggcgttgatg
      181 atctgaacca gcaagttgaa aaagcgttgg aggatttagc aaccaatcct tccgacccga
      241 agttccttgc cgaatatcaa agtgcattag ctgagtatac attatatcga aatgcgcaat
      301 ctaacgttgt aaaagcgtat aaagatctcg attctgcaat catacaaaac ttccgctaaa
      361 ctggataagg attttgttat gtcagtaagt aatatgccac ctattgatag agttgagcaa
      421 tcaactgctc atgaaataca gcaagcgaaa gtgattgatt taaatgaccg ggttctcaat
      481 cttgacaacc cggatgataa aatgatctcc gcttttgcca actatgctgt acaaactgaa
      541 aattggcagc aaaatgcgtt acaagcatta aggtctgata aaaaggggtt aactcctgaa
      601 aaattattag tcttgcagga tcatgtgctt aattataatg tcgaggtttc attggtggga
      661 acattagcaa gaaagattgt cgctgcagtt gagacgttga cccgctcatg aaatatatat
      721 cattattatt gtttatcctg ctgttgtgcg gctgcaagca gcaggaatta ttgaaccatc
      781 ttgatcaaca gcaagctaat gatgtccttg ctgttttaca aagacataat attaatgctg
      841 aaaagaagga tcaaggcaaa acaggatttt caatatttgt cgaaccaacg gattttgctt
      901 ctgcggtgga ttggctaaaa atctacaatc tcccgggtaa gcctgatatt caaatatcgc
      961 agatgttccc tgcagatgca ctcgtttcat ctccacgagc ggaaaaagca aggctatatt
     1021 ctgctattga gcagcggtta gagcaatctc tcaaaattat ggatggcatt gtttcttcga
     1081 gagtacatgt tagttacgat gttgatactg gcgatagtgg taaaactgct ttaccaatt
//
LOCUS       DAZGCQ010000336         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000336 DAZGCQ010000000
VERSION     DAZGCQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.342"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHK07_005394"
     CDS             complement(<1..881)
                     /locus_tag="ACHK07_005394"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8105553.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEFMTGDKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYVGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1138)
                     /locus_tag="ACHK07_005395"
     CDS             complement(871..>1138)
                     /locus_tag="ACHK07_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8105554.1"
                     /translation="WIGAGRMAIDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIF
                     RQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETTPRET"
BASE COUNT          242 a          389 c          279 g          228 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc cgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccgaca
      301 tagctgacat ggcccatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatatcc agcagtacgc cccggacaga ggtgtccgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 atgccaccca gccggtgcac cagcgtgccg gtcaccggca gtacagcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttatct ccggtcatga actcatccgt ttcagccagc
      721 attccgggag catcaagctg tacctgctgt tgtggtaccg aaagacttgc tgcccccatc
      781 tcgcgcccca gcgcgcaaaa gaaaacccgc gcataggcgg gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcaccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttccggcac caatccac
//
LOCUS       DAZGCQ010000337         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000337 DAZGCQ010000000
VERSION     DAZGCQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.343"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1129
                     /locus_tag="ACHK07_005396"
     rRNA            <1..>1129
                     /locus_tag="ACHK07_005396"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          310 a          239 c          360 g          220 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaagg
//
LOCUS       DAZGCQ010000338         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000338 DAZGCQ010000000
VERSION     DAZGCQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.344"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /locus_tag="ACHK07_005397"
     CDS             <1..200
                     /locus_tag="ACHK07_005397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105555.1"
                     /translation="QEGVSVKLRLPHYVGYCTCCPIIIQSAEAKLNPEAVRHGMSDPR
                     KRIQEVRMRQYLHIERGQLTA"
     gene            complement(349..627)
                     /locus_tag="ACHK07_005398"
                     /pseudo
     CDS             complement(349..627)
                     /locus_tag="ACHK07_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            754..840
                     /locus_tag="ACHK07_005399"
     CDS             754..840
                     /locus_tag="ACHK07_005399"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8105556.1"
                     /translation="MVKLALQLGASVARIAQEHDINDNLLFK"
BASE COUNT          313 a          278 c          243 g          289 t
ORIGIN      
        1 ttcaggaagg agtgtccgtt aaactcaggc tacctcatta tgtgggatat tgcacttgct
       61 gcccaatcat cattcagtca gcagaagcaa aattaaaccc tgaagcagtc agacacggaa
      121 tgagtgatcc tcgtaaaaga attcaggagg tcagaatgcg ccaatacctg catatagaaa
      181 gaggccagct cactgcgtaa atcgtcatgg ggctaaaatc cccgaataag agtttgtaaa
      241 atccaagata cagtctgact gtttcaggtt ctttaatccg cgatataacg aagctcccgg
      301 agctgcgtaa ggtctttttg ggctcaatat ttgacaaagg tcataagagt ttttctttcc
      361 gagacagact gcacgaagcg ctctttccgc agcattatta tccgcctccg ccagaccgtc
      421 atcactgtaa taacagaggg catcccactg attcagtaca tagctgaacg cttcgcccag
      481 tctggatttt ttcgacagcg taccattctt ctccaccatc cattcatgca gcgacgtcag
      541 taacactttg cttcgctgct gcctgacgga aagacgctct gactccggta atccccgtat
      601 ttcgtaagcg taaaccgacc gccgtatgta gccattaagc cagtattggt aacgtaggtg
      661 cccacctttt caaactagtg gacgcctgtt atggagcaaa aaacattatc cgcagaaccc
      721 cgcagatcat tttcaaataa ttttaaactt caaatggtta aactggcttt gcaactagga
      781 gcctctgttg cccgtatcgc ccaggaacac gatatcaatg ataacctcct gttcaaatag
      841 ctcagactct ggcaaaacga agggcgtata tctcggcgtc tttcggtaac aacctcttct
      901 gacactggag ttgaattatt acctgtggag atgacgctgg atgagcggcc ttaccccgtt
      961 ttttacgcga ggtaattatg aagatcaatg actagccgtc gaaccgtgtg catgaactgc
     1021 tgcactgaaa cttctcagct gtaaaataat ccttacgcga cgtacttccc ggagcgctta
     1081 cgattaatcg gatgcagtcc tgactggaag aaagaaaaat gga
//
LOCUS       DAZGCQ010000339         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000339 DAZGCQ010000000
VERSION     DAZGCQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.345"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1116
                     /gene="rhsD"
                     /locus_tag="ACHK07_005400"
     CDS             <1..>1116
                     /gene="rhsD"
                     /locus_tag="ACHK07_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO8105557.1"
                     /translation="EPAEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQT
                     VHNPETGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVE
                     YTRDRLHRETVRSFGSMAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWSDNGDL
                     VRISAPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTA
                     WPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRI
                     QHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYDWDGDRLTTVQT
                     DTTRIQTVYEPGSFTPLIRVETENGEREKVQRRSLAE"
BASE COUNT          251 a          331 c          391 g          143 t
ORIGIN      
        1 gtgaaccggc agagcagtgg cagtatgacg gccacggctg gctgacagac atcagccacc
       61 tgagcgaagg ccatcaggtg gcggtgcatt acggttatga tgataagggc cgcctggccg
      121 gggagcgcca gacggtgcat aacccggaga cgggggaact gctgtggcag catgagacag
      181 agcacgcata caacgaacag gggctggcaa accgcgtcac gccggacagc ctgccgccgg
      241 tggagtggct gacgtatggt agcggttacc tggcgggcat gaagctgggc gggacgccgc
      301 tggtcgagta tacgcgggac aggctgcacc gtgagacggt gcgcagcttc ggcagcatgg
      361 caggcagtaa tgccgcatac gaactgacca gcacatacac ccccgcaggc cagttacaga
      421 gccagcacct gaacagcctg gtatatgacc gtgactacgg gtggagtgac aacggcgacc
      481 tggtgcgcat cagcgccccc cggcagacgc gggaatacgg ctacagcgcc acgggcaggc
      541 tggagagcgt gcgcaccctc gcaccggacc tggacatccg catcccgtat gcgaccgacc
      601 cggcgggaaa ccggctgccg gacccggagc tacacccgga cagcacgctc acggcgtggc
      661 cggataaccg catcgcggag gatgcgcact atgtctaccg acacgatgaa tacggcaggc
      721 tgacggagaa gacggaccgc atcccggcgg gtgtgatacg gacggacgac gagcggaccc
      781 accactacca ctacgacagc cagcaccgcc tggtgttcta cacgcggata cagcatggcg
      841 agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg gcaaaacggg
      901 tctggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa ccggaggtga
      961 cgtggtatga ctgggacgga gacaggctga cgacggtgca gactgacacc acacgtatcc
     1021 agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca gagaacggtg
     1081 agcgggagaa agtgcagcgc cgcagcctgg cggaga
//
LOCUS       DAZGCQ010000340         1115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000340 DAZGCQ010000000
VERSION     DAZGCQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.346"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            453..890
                     /locus_tag="ACHK07_005401"
     CDS             453..890
                     /locus_tag="ACHK07_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO8105558.1"
                     /translation="MCELINEASKYGKVNVRKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTISEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            934..>1115
                     /locus_tag="ACHK07_005402"
     CDS             934..>1115
                     /locus_tag="ACHK07_005402"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFO8105559.1"
                     /translation="MYMLGGMSDTSSNRITFERDSQYRITGVSHTDGIRLKLTYHASG
                     YLKAIHRTGNGIQTQG"
BASE COUNT          349 a          184 c          263 g          319 t
ORIGIN      
        1 tgattatata aaagacatga ttgtaaatgt ttcaagaact gaaagattgg atggttattt
       61 tcgtgtgaat gatgatgaag ataatttatt cttagaatgg attgtaaaag atggttattt
      121 agaggaaata attttgtctg gttcataaat tcatcttctt tgttgagaag attaactgcc
      181 taggtgaaat tatcctctat gaccgggacg ctgcaggcca cctggcagaa gtcatcacgc
      241 aggaagacaa gtcgctgtat ccctttgaaa aatccggcag gctaatgggc atcttcagcc
      301 caggcggcac atcacagcga atttgaaggc aaatgcgaaa attagtgatc ttgaagttca
      361 tacagtattg gtaaaaacag gcgaagcttt tccaatgtgt aagaattttc aaatttcaat
      421 atttggcgct gttgttacaa gcgggtgatt atatgtgtga attaattaat gaagcatcaa
      481 aatatgggaa agtcaatgta agaaagatgg acattgattt gtgttgtaaa aaaatagagg
      541 aagaagggta ttctgttttc gttgcgttct atgattttat aaaaaaatat ggcggtttat
      601 atggctttca ttcagcttac aaagacacca ctataagtga ggcttttttt atagatccag
      661 aaagggcctc taatcgcatt tttatagaaa gagtcaatga ttatattgag agagctggtt
      721 acaatatgat tcctgttggc gagtgcagta atggtcatat cacattatta tatggggaaa
      781 ataatgtgat atatggcggc ttggatgatt atctttggaa gtatggggat aatttatctt
      841 catcaattga tatattgatt aatggcaagg aggcagttga aattccgtaa ggccaacttg
      901 atatgacacc tggcacacaa ccccgacgac cggatgtata tgctgggcgg gatgagtgac
      961 acctccagca accgcatcac gtttgagcgc gacagccagt accggatcac aggtgtcagt
     1021 cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct gaaagccatt
     1081 caccgcacgg gtaacggcat acagacgcag ggaaa
//
LOCUS       DAZGCQ010000341         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000341 DAZGCQ010000000
VERSION     DAZGCQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.347"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..419)
                     /locus_tag="ACHK07_005403"
     CDS             complement(<1..419)
                     /locus_tag="ACHK07_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8105560.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMIL"
     gene            complement(317..1060)
                     /locus_tag="ACHK07_005404"
     CDS             complement(317..1060)
                     /locus_tag="ACHK07_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8105561.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
BASE COUNT          252 a          376 c          284 g          200 t
ORIGIN      
        1 aggatcatac tggcccctgc tgcatacaga cccgatacag ccgcggcccc cagccagcca
       61 acagggttcc accatgccac cgcaatcagc gccgcaccca gcaccgcctg aaacacaccg
      121 ccacttttgg cacccgccag acgcggaacg acgtggatca cggcaccatt tgccagcggt
      181 tcattaagac gggcagataa ttcattttcg cctgcatcac gcccggaaat gcgcacctga
      241 taccagccct cattcagttt ctggcgaaac gacggaagct gtgtggccag cgcccggatg
      301 gcttcagccc ccgttttcac acgaaggtcg atgcggcggc caaatcgttg taaatccccg
      361 taaaggcaga tgcgtgccat gcccggtgac gccagaggga gtgtgtgcgt cgctgccatt
      421 tgtcggtata cctctctcgt ttgctcagtt gttcaggaat atggtgcaac agctcgccgt
      481 caccacagta aatggcggca tgattcggca ccgatgaacc aaaacagcac agcagcacat
      541 cgcccggctg cgccgctgac aacggcacct gatacagccc tgtggcctcc agattatcca
      601 gatagagatt ctgaccgtga cgccaccagt catcctcgcg atgaaaatcc ggcatctcaa
      661 tccccgccag atgataagca tcccggaaca gcgtgtaaca gtccgtcacc ccgtgctcaa
      721 agcgccgccc ggtgagatgc ggcacacagc ggaacttatg aatcgtcccc cggcagacca
      781 gccaccacgg caaatcactc tgcacctgca gccgccggtc ggcctcactc agccagggca
      841 gaccaccggg gtggctgtgg accagcgcca caatctcacc ctgcatttct gcctgcagcc
      901 agtcttccgg cgacatacgg aaatacgcct ccggctcacc ggagatattc acgcagggaa
      961 aatatctttc cccctccggc gctctcacca cgaagccgca cgactccgct ggcgcacatc
     1021 gccgggcgtg cgccagaatc gctgattctg tctctgtcat gggattactg cgaaagtttg
     1081 ttaatggaaa ggaagccgcc aaagttgccg ac
//
LOCUS       DAZGCQ010000342         1100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000342 DAZGCQ010000000
VERSION     DAZGCQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.348"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..>1100)
                     /locus_tag="ACHK07_005405"
     CDS             complement(11..>1100)
                     /locus_tag="ACHK07_005405"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO8105562.1"
                     /translation="GAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDNCSIYR
                     VGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQIRKRAP
                     QARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQST
                     FQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEM
                     SKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVA
                     IEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAK
                     QGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          330 a          230 c          181 g          359 t
ORIGIN      
        1 gcctttttat ttattcaaac caattatgaa taacctcttc gaatctttga gttacgccat
       61 cccaactgta gtgatcaaac acaaaatcct gaccttgctt tgcaactgct gttaattcag
      121 gattcgctaa cgtttttaaa atatcgctgc ttatggagtc tgcagtcata ggttctttta
      181 aatggaatcc cgtagtattt tctttgacaa attccgtcat ccccccacgc gtactcacta
      241 aaacaggctt accagcaccc atagcttcaa ttgccaccat gcaaaatggt tcctggaact
      301 gggaaggaat gacgactaaa tctgccagtg ggtaataaca atgcatcttt tcaggcggta
      361 tgcttcctaa cataatgcaa cgatccttca aacgttttgc aatttccctg acattgcgtt
      421 gatatgcacc tttgtcactt ttactcattt ctgtataatc accaatcaca actaatttta
      481 aatttttgtg agcagcggcc aatttttcga atgattgcat caacagcaat atgcctttgt
      541 cagggacgat acgcccagca taaaaaataa ttttctcttc aggggagata ttaagttcgc
      601 ttctagtgat cggctgaaaa gtagattgat aggtttctaa atcaattcca tttggtacaa
      661 tctcaatatc tgcatttgct aaataagatt ggtaatactt tttcaagtat aaactaggca
      721 ctatcatttt aacattttgc tcaagcaatt ttggttcaaa tgcattatgc atatgtataa
      781 ccactcttgc ctgaggagct cgcttacgaa tttgagtgta tagcttcata ctattatgaa
      841 ctacaataac actttctttc gttataggaa aatcatgtgc aatattcaat attctctgtg
      901 aatatggaag aggatcaagt cttgtccatt tttgaaataa ccgcttatat atgcgactga
      961 atccaaccct ataaatacta caattatcat taacaaaggt gtaatttgag tatcctggat
     1021 tcttgataca aacaatacga tttgggaaat tggtacgctg agcaacctga tacatccacg
     1081 tttcaacagc ggccgctccg
//
LOCUS       DAZGCQ010000343         1067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000343 DAZGCQ010000000
VERSION     DAZGCQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.349"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..138
                     /gene="dsbG"
                     /locus_tag="ACHK07_005406"
     CDS             <1..138
                     /gene="dsbG"
                     /locus_tag="ACHK07_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Involved in disulfide bond formation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO8105563.1"
                     /translation="DNEKLMDDLGANVTPAIYYMSKENTLQQAVGLPDQKTLNIIMGN
                     K"
     gene            347..>1067
                     /locus_tag="ACHK07_005407"
     CDS             347..>1067
                     /locus_tag="ACHK07_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8105564.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAV"
BASE COUNT          356 a          219 c          200 g          292 t
ORIGIN      
        1 gacaacgaga aactcatgga cgatctgggg gcaaatgtca ctccggctat ctattacatg
       61 agtaaggaaa atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc
      121 attatgggaa ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa
      181 ccatcctaaa aaagcatgaa ttgctcgctc tgccctaagt ggctgtgcta ttttagaaaa
      241 acttaatcct gagttaagca aaaaaacata atcaataaaa atatatacgc aaaaaacatt
      301 gattaagtga atatatcatg gaagaaaaat ataaccggag tagtgtatga ccaatctcta
      361 cgacttgaaa aagttcgact taaatcttct tgtcatattt gagtgtattt accagcattt
      421 aagcattagc aaagctgctg aatcactata tatcacccca tcagccgtga gccagtcgtt
      481 acaacgctta cgtgcgcaat ttaatgaccc tgtattcatt cgctcaggaa aaggtatcgc
      541 gccgacgaca acagggttaa atctgcatca ccatcttgag aaaaatctca gaggccttga
      601 acaaacaatt aatatagtca ataagtccga actaaagaaa aactttatta ttcacggccc
      661 tcagctcatt tcttgctcta ataatagtat gctgatccgc tgcctacggc aagattcctc
      721 tgtcgagatt gagtgccatg atatcctcat gtcagcagag aatgctgaag aactgttggt
      781 tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg atcagccgtt ccgtcatttg
      841 catgccattg catacgattc gcaatactct aatttgtagt aataggcatc cacgaattac
      901 tgacaatagc acttatgaac aaattatggc agaagagttc acccaattaa tatctaaatc
      961 agcaggggtt gatgatattc aaatggaaat agacgagaga tttatgaatc gcaaaatttc
     1021 atttcgtggt tcctctttgc tgacaatcat taatagcatt gcagtta
//
LOCUS       DAZGCQ010000344         1062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000344 DAZGCQ010000000
VERSION     DAZGCQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.350"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..306)
                     /locus_tag="ACHK07_005408"
     CDS             complement(91..306)
                     /locus_tag="ACHK07_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8105565.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            complement(382..654)
                     /locus_tag="ACHK07_005409"
     CDS             complement(382..654)
                     /locus_tag="ACHK07_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8105566.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEACLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAESGELQPESPVSDATEPQPESVMML"
     gene            complement(680..862)
                     /locus_tag="ACHK07_005410"
     CDS             complement(680..862)
                     /locus_tag="ACHK07_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8105567.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
BASE COUNT          234 a          305 c          240 g          283 t
ORIGIN      
        1 aggcatcagc gtcgacagtt ttttattcag ttcagcaatt tcattttcca ggcgactgaa
       61 gcgctgattc atttcttcat ggttcattat tcactctccc cgggcggcct tacgccggtc
      121 ttctttaatt ttgaaataca ggttcgtcag gtacgtcagc agcccaaaca gcagactccc
      181 cagcacacct attgccgccc actgagacgg ggaaaccctg tccagcaact gcaggaacca
      241 gtagcccgtt cccaccgctg acgtggtgta tgacacacct gttgtgattt tttccatctg
      301 gtacataccc cgtctcccgc acacggaagc tcacaacatg aaaaaggcca gcagcagttt
      361 actgatggcc ccgacacccc gttacagcat catgaccgat tcgggttgcg gttcagtcgc
      421 atcggagacc ggtgattcag gctgaagttc accgctctct gcggtggtat ctcccgcttc
      481 agtcggtggc tctgccggta cgccaagcag ctcatccaga atggcatcaa cttctgcatc
      541 aagacacgcc tcaaggttct ggcggagttt ctgtttcagt gcgctcagga cttcttcaga
      601 gcgcaggact tccttcactg cctctgcagt gaccagggat gtgatttctg acatgggatt
      661 ttctcgctga aaggggttgt taaggagtaa cgggctcttc gggtttgctt ccggctgact
      721 gactggcgct gattttctct gcggcccttt tatcaatctg cctgcgccag aaatcgcgca
      781 ctgccctgta cccacccgaa aggagataca gcacacagac cgccgtacag aagtacaaca
      841 tcacctgatg aataaatgtc ataatttctt accgttatgg ttgacaatga gaactgtttt
      901 cattaaaaaa acaaccgatg tacgaaagca tcttttcttt acattctcca ttgggattac
      961 ctccgccagc ttccattcct gccgctggcg gcttttttta tcatgccgcg acgtccgcgt
     1021 tgttcacttc caccttcaca ctgtcaatca gcagcgtata tg
//
LOCUS       DAZGCQ010000345         1062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000345 DAZGCQ010000000
VERSION     DAZGCQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.351"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1062)
                     /locus_tag="ACHK07_005411"
     CDS             complement(<1..>1062)
                     /locus_tag="ACHK07_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8105568.1"
                     /translation="WQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPE
                     TGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEFTRDR
                     LHRETVRSFGNNAYELTSTYTPTGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTR
                     EYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRITEDA
                     HYLYRYDEYGRLTEKTDRIRAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESR
                     YLCDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRIQTVYE
                     PGSFTPLIRVETENGERKK"
BASE COUNT          145 a          360 c          316 g          241 t
ORIGIN      
        1 cttttttccg ttcgccgttc tctgtctcga cccggatgag cggcgtgaag cttcccggct
       61 cgtataccgt ctggatacgt gtggtgtcag tctgcaccgt cgtcagcctg tcgccgtccc
      121 atccgtacca tgtctcctcc ggtttacgcg acagcgacat ccaccccgtc aggtcacgct
      181 cccgccgcca gacccgtttt gccattcgcc gtcccagcgg gtcacagagg tagcggctct
      241 cgaccagtgg ctcaccatgc tgtatccgag tataaaacac caggcggtgc tgactgtcgt
      301 agtggtagtg gtgggtccgc tcgtcgtccg tccggataac ccctgcccgg atgcggtccg
      361 tcttctccgt cagcctgccg tactcatcgt agcggtacag gtagtgcgcg tcttcggtga
      421 tgcggttatc cggccacacc gtgagcgtgc tgtcaggatg cagctccggg tccggcaggc
      481 ggttgcccgc cgggtcggtg gcatacggga tgcggatgtc caggtccggt gcgagggtgc
      541 gcacgctctc cagcctgccc gtggcactgt agccgtattc ccgcgtctgt cgcgggccgc
      601 tgatgcgcac caggtcgccg ttgtcattcc acccgtagtc acggtcatac accaggctgt
      661 tcaggtgctg gctctgtaac tggcctgtgg gggtgtaagt gctggtcagt tcatatgcat
      721 tattgccgaa gctgcgcacc gtctcccggt gcaggcggtc gcgcgtgaac tccagcagcg
      781 gcgtcccgcc cagcttcatg cccgccaggt aaccgctgcc atacgtcagc cactccaccg
      841 gcggcaggct gtccggcgtg acgcggtttg ccagcccctg ttcgttgtat gcgtgctctg
      901 tctcatgctg ccacagcagt tcccccgtct ccgggttatg caccgtctgg cgctccccgg
      961 ccaggcggcc cttatcatca taaccgtaat gcaccgccac ctgatggcct tcgctcaggt
     1021 ggctgatgtc tgtcagccag ccgtggccgt catactgcca ct
//
LOCUS       DAZGCQ010000346         1050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000346 DAZGCQ010000000
VERSION     DAZGCQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.352"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..449
                     /locus_tag="ACHK07_005412"
     CDS             <1..449
                     /locus_tag="ACHK07_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO8105569.1"
                     /translation="VDDKVDLFSGIERYLLGVIEKNNSYNEENYGNKKDKYAKLIYER
                     NYLVATKDQLINESFELNNKDNKDKKDVARINEIKEILKTIDGKLPNISDEILHLKDE
                     TERLEEEYQQENTLNDVVQNIRRWLKENENMVKAIKKIDTNNMANK"
BASE COUNT          395 a          153 c          200 g          302 t
ORIGIN      
        1 atgtagatga taaagtcgat ttattctctg gcattgaacg atatttactg ggggtaattg
       61 agaaaaataa tagctataat gaggaaaatt atggtaataa aaaagacaaa tatgccaagc
      121 ttatttatga aagaaattat ttagtcgcta ctaaggatca attgattaat gagagttttg
      181 aattaaataa taaagacaat aaagataaaa aagatgtagc cagaattaat gagattaagg
      241 aaatactaaa gacaatagat ggaaaactac caaatataag tgatgagatt ctacatctta
      301 aggatgaaac agagagattg gaggaagagt atcaacaaga aaatacactt aatgatgtag
      361 ttcaaaatat caggcgttgg ctaaaagaaa atgaaaatat ggtgaaagct attaagaaaa
      421 ttgatactaa taacatggcg aataaataaa cggatcaagt cgttcctgtg acaatgaggt
      481 tatacgaaaa agacgcttac gcattagaaa tctggtgtac tttaattaaa ccagtgtcgc
      541 tgatgtgtaa gcgtcgacgt tgggagacga ttaaattgtt atatcaccgc tacgcccttt
      601 ccagacggcg tataagagtg caatatgttg agttttccga gtgaatggtg cattttatta
      661 accgagccat ttgaataagc caacgcaata aaacagactg gaataataag tctgtattta
      721 ttgttcatca caatcgcact ctacggtttt atcctgctga aatgagacag cctccagcgt
      781 ttgtggatga tccttgaacg agagtcatgg tctggtgtca ggattcacaa atgacctgtt
      841 tcctgacatg gatataaacc caacatagag tgcggtagtt aatcttaaaa atctatagtc
      901 ggctactcag cacaaaacat cattattgat tctttataag aaagaatctc gccatcattt
      961 ttatataaat cactattaag gaaatccaga aactcttgcc ttacactata aatattagct
     1021 ttttggtcaa ctaatgataa taatatatcc
//
LOCUS       DAZGCQ010000347         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000347 DAZGCQ010000000
VERSION     DAZGCQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.353"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..>1022
                     /locus_tag="ACHK07_005413"
     CDS             61..>1022
                     /locus_tag="ACHK07_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105570.1"
                     /translation="MLQIVGALILLITGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRR"
BASE COUNT          242 a          251 c          259 g          270 t
ORIGIN      
        1 gatattcaac gccacattcg ctggcattaa caataacatg atattcatca cggagtgact
       61 atgttacaga tagtcggcgc gctgatcctg ctgatcacag gatttgccat tcttcgcctt
      121 ttgttcagag cattaatcag cacggcttct gcgctggcag ggctcatatt gctgtgtctg
      181 ttcggcccgg ccttactggc tggctatatc accgaacgca taacccggtt gttccatatt
      241 cgctggctgg caggcgtatt tctgacgatt gccggaatga tcatcagctt catgtgggga
      301 cttgatggta aacatatcgc gctggaggct cacacctttg actctgtgaa atttattctg
      361 accactgctc tcgccggtgg tctgctggct gttcccctgc agatcaaaaa cattcagcag
      421 aacgggatca caccagaaga tatcagcaag gaaattaacg ggtattactg ctgtttttat
      481 actgcctttt tccttatggc gtgttctgca tgcgcaccat tgatcgcgtt acagtacgat
      541 atttcaccgt cactgatgtg gtggggcggg ttgttgtact ggctggctgc attagtgacg
      601 ctgctatggg cggccagcca gatccaggcg ctgaaaaaac tgacctgtgc catcagccag
      661 acactggaag aacaaccggt gctcaacagt aaatcgtggc tgaccagttt gcaaaacgat
      721 tacagccttc ctgactcact gacggagcgc atctggctga cgctcatttc tcaacggatt
      781 tcccggggag agctgaggga atttgaactg gcagacggaa actggttact gaacaatgcc
      841 tggtatgaaa gaaacatggc agggtttaac gaacagttga aagagaacct gtcattcaca
      901 cctgatgaac tgaaaacgct cttccggaac cgcctgaatt tatcaccgga agcgaatgac
      961 gattttctcg atcgttgcct ggacggcggt gactggtatc ccttttcaga aggtcgccgt
     1021 tt
//
LOCUS       DAZGCQ010000348         1013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000348 DAZGCQ010000000
VERSION     DAZGCQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.354"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1013
                     /locus_tag="ACHK07_005414"
     CDS             <1..>1013
                     /locus_tag="ACHK07_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HFO8105571.1"
                     /translation="YEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQL
                     VKKNQTRITGMDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVE
                     WQNRPLDAVYPIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGA
                     KFWLNVLTELKNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVS
                     WKDYKAVTRDLKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWPNLATFFAY
                     PTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMSLKD
                     WRM"
BASE COUNT          272 a          257 c          271 g          213 t
ORIGIN      
        1 gtatgagaaa aatcagtcca ggccaggagc taactcccgc aacggttatt ccacaaagac
       61 cgttatcaca ggcgacggtc cactggaact gcgtactccg cgcgatcgtg acggtacctt
      121 cgaaccacaa ctggtaaaga aaaatcagac ccgtattacc gggatggata accagatcct
      181 ctcgttgtat gccaaaggga tgaccacccg tgagatagct actgcgttca aagaactgta
      241 tgacgcagat gtttcaccgg cactgatatc aaaagtcacc gatgccgtga tggagcaggt
      301 tgtagaatgg caaaacagac cactggatgc tgtttacccc attgtttatc ttgactgtat
      361 cgtcctgaaa gttcggcagg acagtcgcgt catcaacaaa tcggtgttcc tggcactggg
      421 catcaatatc gaaggtcaga aagaactgct gggtatgtgg ctggccgaaa atgaaggggc
      481 gaagttctgg ctcaatgtgc tgactgaact gaaaaaccgc ggtctgaacg atatcctcat
      541 cgcctgtgtg gatggcctga aaggcttccc ggatgccatc aacacagtat atccgaaggc
      601 ccgcatccag ttatgcatcg tgcatatggt gcgcaacagc ctgcgcttcg tgtcatggaa
      661 ggactacaaa gccgtcactc gcgacctgaa agcgatttat caggctccca cggaagaggc
      721 aggtcagcag gcactggaag cgttcgctgc ggcctgggac agtcgctatc ctcagataag
      781 ccgaagctgg caggctaact ggccgaatct tgccacgttc ttcgcttatc caacggacat
      841 ccgcaaagtg atctatacga cgaatgccat cgagtcgcta aacagcgtga tccgccatgc
      901 gatcaaaaag cgtaaagtgt tcccgacaga cgactcggtg aaaaaagtgg tgtggctggc
      961 aatccagtct gcgtcccaga aatggacgat gtcgttgaag gactggcgaa tgg
//
LOCUS       DAZGCQ010000349         1009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000349 DAZGCQ010000000
VERSION     DAZGCQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.355"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..344)
                     /locus_tag="ACHK07_005415"
     CDS             complement(<1..344)
                     /locus_tag="ACHK07_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8105572.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLCDGWYQVRIAGRDVSTSGLTVQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVL"
     gene            complement(341..>1009)
                     /locus_tag="ACHK07_005416"
     CDS             complement(341..>1009)
                     /locus_tag="ACHK07_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8105573.1"
                     /translation="STPEGERYFPCVNISGEPEDYFRMAPEDWLQAKMQGEIVALVHS
                     HPGGLPWLSEADRRLQVQSDLPWWLVCRGVIHKFRCVPHLTGRRFEHGATDCYTLFRD
                     AYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEATGFYRVALTEAQPGDVLLCCFGSSVP
                     NHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLAAA
                     STFV"
BASE COUNT          233 a          333 c          264 g          179 t
ORIGIN      
        1 agcaccatac tggcaccgag agaaaacagg atgccggtca taccaccggc cccaatggct
       61 gccccccatg ctgcaagggt ggctccggca gtaaagaatg atccggcaat ggcggcagct
      121 cccaggacaa tctggaatac gccacctgac ttggccccgg cgactctggg aacaatatga
      181 atcacagcgc catcaggcag agtctcatgt aactgcaccg ttaacccgga cgtgctgacg
      241 tcccgcccgg caatccgtac ctgataccag ccgtcgcaca gtttctgacg aaacgccggg
      301 agctgtgtgg ccagtgcccg gatggcttca gcccccgttt tcacacgaag gtcgatgcgg
      361 cggccaaatc gttgtaaatc cccgtaaagg cagatgcgcg ccatgcccgg tgacgccaga
      421 gggagtgtgt gcgtcgctgc catttgtcgg tatacctctc tcgtttgctc agttgttcag
      481 gaatatggtg cagcagctcg ccgtcaccgc agtaaatagc ggcatgattc ggcaccgatg
      541 aaccaaagca gcacagcagc acatcgcccg gctgcgcctc tgtcagtgcg acacggtaaa
      601 aaccagtcgc ctccatattg tcaagataga gattctgacc gtgacgccac cagtcatccc
      661 cgcgatgaaa atccggcatc tcaatcccag ccagatgata agcatcccgg aacagcgtgt
      721 aacagtccgt cgccccgtgc tcaaagcgcc gcccggtgag atgcggcaca cagcggaact
      781 tatgaatcac tccccggcag accagccacc acggcaaatc actctgcacc tgcagccgcc
      841 ggtcggcctc actcagccag ggcagaccac cggggtgact gtggaccagc gccacaatct
      901 caccctgcat tttggcctgc agccagtcct ccggagccat ccggaaataa tcctccggct
      961 caccggaaat attcacgcag ggaaaatatc tttccccctc cggcgtgct
//
LOCUS       DAZGCQ010000350          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000350 DAZGCQ010000000
VERSION     DAZGCQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.356"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..277
                     /locus_tag="ACHK07_005417"
     CDS             <1..277
                     /locus_tag="ACHK07_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105574.1"
                     /translation="EFYEITEDSENLELQLEREVLSKFMPLGSCSITRLHIYTGVLYK
                     IDGYTRKQITRAIKSLKEKGILENANKSSFTAINKFCVGEVKKILGR"
     gene            279..>999
                     /locus_tag="ACHK07_005418"
     CDS             279..>999
                     /locus_tag="ACHK07_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT protein"
                     /protein_id="HFO8105575.1"
                     /translation="MINFSFSDTLRVDIENLISEKLNSLGIMFRLFSRNKDAQSLERK
                     LENPKYGVTHKVQDALGVRIALYFNDDVELVHSILNEIFTEREKDHSIDIMKTAQFSA
                     VRYNIIYELPSHLLLKEYAYTKVSEKIDSTFELQIRSILSEGWHEVEHDLRYKAQYDW
                     DGHDTESRKLNGIYATLETSEWTMIKIFDELAYKHYKNKNWNGMLRQKLRLRLGTQEL
                     HHDIINVLNNNPGVAKDIFRIS"
BASE COUNT          383 a          131 c          189 g          296 t
ORIGIN      
        1 tgaattttat gagataacag aagacagtga aaatttagaa ttacagttag aaagagaagt
       61 cctttctaaa ttcatgcctt taggttcatg ttctataact cgtttgcata tttatacagg
      121 tgttctatat aaaatagatg gttatacaag aaagcaaata acacgagcta taaaatcgct
      181 taaggagaaa ggcattttag aaaatgcaaa taaaagttct tttacagcga ttaataaatt
      241 ttgcgtaggt gaagtcaaaa agattttggg gcgttaaaat gatcaacttt agttttagcg
      301 atacattgcg agttgatatt gaaaatttga tatcagaaaa gctcaatagc ttaggcataa
      361 tgtttagatt attttccaga aacaaggatg cacaatcact tgaaagaaaa ctggaaaatc
      421 ctaaatacgg tgttacgcat aaagtacaag acgcgttagg tgttagaatt gcattatatt
      481 ttaatgatga tgtggagtta gttcatagta tcttaaatga aatatttaca gagcgagaaa
      541 aagatcatag cattgatata atgaaaacag cacaattttc agcagttaga tacaatataa
      601 tatatgaact accttctcac ttacttctaa aagaatacgc atatacaaag gtttctgaaa
      661 aaatagattc aaccttcgag ttacaaataa gatctatctt atccgaagga tggcatgaag
      721 tcgagcatga cctacgatat aaggcgcaat atgactggga tggtcatgac actgagagca
      781 ggaagttgaa tggtatatat gcaacgttag aaacgtctga atggacaatg atcaagattt
      841 ttgatgagtt agcatataaa cattataaaa ataaaaactg gaatggtatg cttcggcaga
      901 aattacgatt aaggttaggt acgcaagagt tacatcatga tatcataaac gtcctaaata
      961 ataaccctgg agtagcaaaa gatattttta ggatttctc
//
LOCUS       DAZGCQ010000351          989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000351 DAZGCQ010000000
VERSION     DAZGCQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.357"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..791
                     /locus_tag="ACHK07_005419"
     CDS             <1..791
                     /locus_tag="ACHK07_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8105576.1"
                     /translation="TEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRH
                     RYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDPLGLDLLSLPSPRQKPVSQSCT
                     IDPINQQPVGRFIGDSKGNIMVEPVGGRTIPYPPSNPGSADTHTLYQNNSNAYRLNPI
                     GHANNSTPHAHAHLPGTGSGMKGQGPSLDVNGRVVPWNSSAAHIGIRSLGTLSFFSSV
                     LHMFTLRSCREGTFGQCYCAMYVEMSTGSNQDEADIMCGMPEYF"
     gene            895..>989
                     /locus_tag="ACHK07_005420"
     CDS             895..>989
                     /locus_tag="ACHK07_005420"
                     /inference="COORDINATES: protein motif:HMM:NF040040.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFO8105577.1"
                     /translation="MSRIAYGTTEWNFYDNEDLEDGYLVFSDVSF"
BASE COUNT          299 a          176 c          227 g          287 t
ORIGIN      
        1 ctacagaatg gtgcgcagaa tacgatgagt ggggcaacct gctgaatgaa gagaacccgc
       61 atcagctgca gcagcttatc cgcctgccgg ggcagcagta tgatgaggag tccggcctgt
      121 attacaaccg ccaccgctat tatgacccgc tgcaggggag gtatatcact caggatccga
      181 ttggactgaa ggggggatgg aatttttatc agtatccgtt gaatccggtt cagtatatag
      241 accctttggg attagatcta ttatcattac cttctcccag acaaaaacct gtaagtcaat
      301 cctgtactat tgatcctata aatcaacaac ctgttggtag gtttataggt gattctaaag
      361 gcaatattat ggttgaaccg gtaggtggca gaacaatccc gtatcccccc agtaatccag
      421 gaagtgcaga tactcacact ctttatcaga ataattcaaa tgcttatagg cttaatccaa
      481 tagggcatgc aaacaattca acaccacatg cacatgcaca tttaccagga acaggtagcg
      541 gtatgaaagg ccaaggtcca tcccttgatg ttaatggtcg cgttgtacca tggaatagta
      601 gtgccgcaca tatcggtatt agatcactcg gtacactaag cttttttagt tctgttttac
      661 atatgtttac acttagatca tgtagagagg gaacgtttgg acaatgttat tgtgctatgt
      721 atgttgaaat gtcaacggga agcaatcaag atgaggctga tataatgtgc ggaatgcctg
      781 agtattttta gaataggggg gtaatatgtt tgatagaata tatcttggtg atcgagcaat
      841 aaaaaaattg aatttgattt atggagtaaa caaattagaa ttcaggttaa tttaatctca
      901 aggatagcct atggtacaac agaatggaat ttttatgata atgaagactt agaagatggt
      961 tatttggtat tttcagatgt tagtttctt
//
LOCUS       DAZGCQ010000352          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000352 DAZGCQ010000000
VERSION     DAZGCQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.358"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..244)
                     /locus_tag="ACHK07_005421"
     CDS             complement(<1..244)
                     /locus_tag="ACHK07_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105578.1"
                     /translation="MTLFLFVKCKAACVTSLYEVQQSLFTYIYLIRQDPVHFDSYIKN
                     VHNELKIFHDIDKAMNIASDMVKIIDIKTRLSFNNDL"
     gene            complement(325..>987)
                     /locus_tag="ACHK07_005422"
     CDS             complement(325..>987)
                     /locus_tag="ACHK07_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8105579.1"
                     /translation="NEENPHHVYQPYRLPGQQYDEESGLYYNRNRYYDPLQGRYITQD
                     PIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWTYGEVGNSCVTSQRDFTPPPGLADM
                     KASIGAACSRNNASRNCHPVDGSNASAPSDKAAWENIQNATNGKDLSGGGNMFCVGNE
                     DCAVVHSCREIVNARHPAGRVRERVTPLTTSGTATLNGTTVYFYNDPRNGNCPAHEYI
                     PQ"
BASE COUNT          283 a          224 c          190 g          290 t
ORIGIN      
        1 ataaatcatt attaaatgat aaacgggtct ttatatcaat aattttgacc atatctgagg
       61 ctatattcat ggctttatca atatcatgga atatttttaa ttcgttatgc acattcttaa
      121 tatatgaatc aaaatgaaca gggtcttgtc taatcaaata aatgtatgta aagagcgatt
      181 gctgaacctc atataaggaa gttacacacg ctgctttaca tttaacaaat aaaaataatg
      241 tcataacgca acatgttata aaaaaccttt tcatgaaaag gatctccaaa aaaggtggct
      301 agagcttagt ctggccacct ttctttattg aggaatatat tcgtgagcag gacaattacc
      361 attccgggga tcattataaa agtaaactgt tgttccattg agcgttgctg ttcctgacgt
      421 tgttaacgga gttacacgtt cacgcactcg tcctgccggg tgtcgagcat taactatttc
      481 acgacagcta tgaacaaccg cacagtcctc atttcctaca caaaacatat ttccaccacc
      541 agataaatct ttaccatttg tcgcattttg aatattttcc catgcggctt tatccgatgg
      601 tgcagaagca ttactaccat ctacggggtg gcagtttctg gaagcattgt ttcttgagca
      661 ggcagcgcca atgctggctt tcatatccgc aagtccgggc gggggagtaa aatcgcgttg
      721 actcgtaaca cagctattac ctacctcacc ataggtccaa ttgtcctctt gagttaaacc
      781 tgttgggtca ataaactggg ctggattatt aagcacataa ctatagggat tccacccccc
      841 caccggccca atcggatcct gggtaatata cctcccctgc aacggatcat agtaccggtt
      901 gcggttatag tacagccccg actcctcatc atactgctgc cccggcaggc ggtatggctg
      961 atacacatga tgcgggttct cctcatt
//
LOCUS       DAZGCQ010000353          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000353 DAZGCQ010000000
VERSION     DAZGCQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.359"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            512..>987
                     /locus_tag="ACHK07_005423"
     CDS             512..>987
                     /locus_tag="ACHK07_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8105580.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG"
BASE COUNT          229 a          256 c          276 g          226 t
ORIGIN      
        1 attgctggtt tcgtggtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
       61 cagttgaagg ttgtctgccc gactgagaat ttgttaaaaa aaatcctgca tggtgaatcc
      121 ccctgagcgg cggggcatat cagcgtcaca ggtgtttctg ttttacctct atcctttctg
      181 tgcgggttca ggtgctgata ctgaactcac cgggaggcac ccggcaccat gcatgaacgg
      241 tacatagcgc atacatcagc ccctctccgg aggggctttc ttgtgggcaa aaaaaagccc
      301 gcgccgggag acgcgggcgg caaggaataa acaacaaaac gtgaagtaat atttcagctg
      361 gcgaataata cccgacagta atcactctgt gcaactgcgc ggcctttttc gtattgcggg
      421 ctgtagtctc ctttctgcca ttgtcctgta acttccggac ttcagcccgc tccttatctg
      481 actcacaaca ttatcccgac cgggaggatt catgacattt aaacactacg atgtggtcag
      541 ggcggcgtcg ccgtcagacc ttgcggagcg actgactcaa aaactgaagg aggggtggca
      601 gccatttggc agtcctgtcg ccatcacgcc ttataccctg atgcaggcca ttgcggcgga
      661 aggtgatgtc accacacctg tgctggtgaa gccgtcggat ggagaaggca cagttatcag
      721 caccaccagc gaaccggagt attactttgt tgttgttctg gcggggcagt caaacagcat
      781 ggcatatggt gaaggccttc cgctgccgga gacatatgac cgtccggacc cgcgtattaa
      841 gcagctggcg cgccgcagta cggtgacacc gggcggtgcc gcctgtaagt ataacgacat
      901 cattccggcg gaccattgtc tgcatgatgt gcaggacatg agccggctga atcatccgaa
      961 agcggacctg tcaaaggggc agtacgg
//
LOCUS       DAZGCQ010000354          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000354 DAZGCQ010000000
VERSION     DAZGCQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.360"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..225
                     /locus_tag="ACHK07_005424"
     CDS             <1..225
                     /locus_tag="ACHK07_005424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105581.1"
                     /translation="QLNPAGLSYTYCYEQNRITITDSLDRREVLHTQGEGGLKRDTVR
                     CVGKGHPYHAGRADAQYGIRCCRTGHPPDQ"
     gene            116..972
                     /locus_tag="ACHK07_005425"
                     /pseudo
     CDS             116..972
                     /locus_tag="ACHK07_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          233 a          312 c          284 g          143 t
ORIGIN      
        1 cagcttaacc cggcgggctt aagctacacc tactgttatg agcaaaaccg catcaccatc
       61 accgacagcc tggaccgccg tgaagtgctg cacacgcagg gcgaaggcgg gctgaagcgg
      121 gacacagtac gatgcgtggg gaaaggccat ccgtaccacg cagggcgggc tgacgcgcag
      181 tatggaatac gatgctgccg gacgggtcat ccgcctgacc agtgaaaacg gcagccatac
      241 caccttccgt tacgatgtac tcgaccggct gatacaggaa accggctttg acggccgcac
      301 acagcgttat caccacgacc tgaccggcaa acttatccgc agcgaggatg aggggctggt
      361 cacccactgg cactatgacg aagcagaccg cctcacgcac cgcaccgtga atggcgaaac
      421 cgcagagcgg tggcagtatg acgaacgcgg ctggctgaca gacatcagcc atatcagcga
      481 agggcaccgg gtgacggtgc attacgggta tgatgagaaa ggccggctga ccggtgagcg
      541 tcagacggtg catcacccgc agacggaagc actgctctgg cagcatgaga ccagacacgc
      601 ttacaacgcg caggggctgg cgaaccgctg tataccggac agcctgcccg ccgtggaatg
      661 gctgacctac ggcagcggct ggctgtcagg catgaagctc ggcgacacac cgctggtgga
      721 tttcacccgc gaccgcctgc accgggaaac gctgcgcagc ttcggccatt atgaactcac
      781 caccgcttat acccctgccg ggcagttaca gagccagcac ctgaacagcc tgcagtatga
      841 ccgcgattac acctggaacg acaacggcga actcatccgc atcagcagcc cgcgccatac
      901 ccggagttac agctacagca actccggcag gctgaccggc gttcacacca ccacctcaac
      961 tctggatatc cg
//
LOCUS       DAZGCQ010000355          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000355 DAZGCQ010000000
VERSION     DAZGCQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.361"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..252)
                     /gene="stxB2"
                     /locus_tag="ACHK07_005426"
     CDS             complement(<1..252)
                     /gene="stxB2"
                     /locus_tag="ACHK07_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit B"
                     /protein_id="HFO8105582.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEV"
     gene            complement(265..>947)
                     /locus_tag="ACHK07_005427"
     CDS             complement(265..>947)
                     /locus_tag="ACHK07_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HFO8105583.1"
                     /translation="EQNNLYVAGFVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYT
                     TLQRVAALERSGMQISRHSLVSSYLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQ
                     IQREFRLALSETAPVYTMTPEEVDLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAI
                     LGTVAVILNCHHQGARSVRAVNEEIQPECQITGDRPVIRINNTLWESNTAAAFLNRRA
                     HSLNTSGE"
BASE COUNT          249 a          235 c          185 g          278 t
ORIGIN      
        1 cacttcagca aatcctgaac ctgacgcaca ggtatttgat ttgattgtta ccgtcattcc
       61 tgttaactgt gcgctttgca gtagcggttg cagattccag cggttagtcc agtactcttt
      121 cccggccact tttactgtga atgtatcatt ctcattatac ttagagaact caattttacc
      181 ttttgcacaa tcagctgcca ttgcattaac agaaacaaaa gcaaataaag ccgctacaaa
      241 tatcttcttc atatttaact cctgttattc tccggatgta tttaaagagt gagccctgcg
      301 attcagaaaa gcagctgcgg tattactttc ccataaagta ttgtttatcc ttataactgg
      361 cctgtcgcca gttatctgac attctggttg tatctcttca ttcacggcgc gaacggaacg
      421 cgccccctga tgatggcaat tcagtataac cgccactgtg cccagtatcg ctgatatatt
      481 attaaaggat attcgcccca ctctgacacc accctctccc cgaaactccg gaagcacatt
      541 gctgattctc ccccagttca gtgtgaggtc cacttcttcc ggtgtcatcg tataaacagg
      601 agcagtttca gacagtgcca gacgaaattc tctctgtatt tgcctgaacc gtaaggcttc
      661 tgctgtgaca gtgacaaaac gcagaactgc tctggatgca tctctggtca tggcatttcc
      721 actaaactcc attaacgcca gatatgatga aaccagtgag tgacgactga tttgcattcc
      781 ggaacgttcc agcgctgcga cacgttgcag agtggtataa ctgctgtccg ttgtcatgga
      841 aacagttgtc acaccgggca ctgatatatg tgcaaaatct gaaaatctgt agaaagtatt
      901 tgttgccgta ttaacaaatc cggccacata taaattattt tgctcaa
//
LOCUS       DAZGCQ010000356          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000356 DAZGCQ010000000
VERSION     DAZGCQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.362"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>947)
                     /locus_tag="ACHK07_005428"
     CDS             complement(<1..>947)
                     /locus_tag="ACHK07_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8105584.1"
                     /translation="IIPDNNHVIKLSSPITVTIERKGGYYFINFSGDKSDIIYKVDGF
                     SIKERNFFTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNA
                     NTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLF
                     TLLDKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNME
                     GNKDSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSETKV
                     LTSHHGIIRDEENKCYCLGALQVPFNQMKNPTDPEELAY"
BASE COUNT          306 a          186 c          142 g          313 t
ORIGIN      
        1 tatatgctaa ttcctcagga tcagtaggat ttttcatctg attaaatgga acctgcaaag
       61 caccgagaca ataacattta ttttcttcat cccttattat tccgtgatga ctggttaaaa
      121 ctttcgtttc tgagtcataa ttacaaacat cgagcgtttt aattaagtta tatgtatcac
      181 cggaagagag tttatgattt gctagtcgtg cactatagtt accattaaaa ttgatttcat
      241 gatttccagc cagaacgacg acatttttat ttatacgact atctttatta ccctccatat
      301 ttcgcataga atttagcaaa gtaaggatat atttatcacc aaaaatagta ctgaatctat
      361 cgcctgtatg atccccaagg aagatacaag gggtaatcgc atcgcctttt aaaaaaacat
      421 gttgatgcaa ctcattcaag atcatgtctc tcttatctaa taaagtgaag agttttttca
      481 attttttatt ttcatatgcg aatgaagcca tgtcttcgtg tttcaataaa tcacataata
      541 aactccaccc cttttccctg atgcctatat gccctgaaac gagcaatgca taaagtacag
      601 cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca
      661 caccacactg tttataaaag tctatttttg aaaaaacaga ttcaacatag ctatcaaact
      721 tttccttagt aaacgtctct ttagagacag cccaacgcca gtcaggtttg aaattaccac
      781 taagaagagt aaagaaattt ctctctttga tagaaaaccc atcaacttta taaattatgt
      841 ctgatttatc cccggagaaa ttaataaaat aataaccgcc cttacgctca atcgtaacgg
      901 taatgggaga cgataatttt atcacatggt tattgtctgg tataatt
//
LOCUS       DAZGCQ010000357          942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000357 DAZGCQ010000000
VERSION     DAZGCQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.363"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>942
                     /locus_tag="ACHK07_005429"
     CDS             <1..>942
                     /locus_tag="ACHK07_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO8105585.1"
                     /translation="KYCSFKNVIFSGVINSPNLEGPVFENCYFDDCKFNNIQLYETND
                     DTVGSGNKKPIKGMFKGCFISKCKIENYRCETSKIYNVTQPVSINEKLGSYLFMQSFV
                     QDCTIQGGYCPGSSILLSHFYNCTIAKLDAHGMDFLANAFTKSSDNEARAQDQGTVFN
                     YCNLNSINMKCSLGNASSDEYKFNPEEYFSDYIERRSRTLKLDDNFSNCVGNIEKAVK
                     TSFSKQNSENDNISFNYCDLRSANLDSYYREDWCKDCSIDPDSIYLEDGVTPIKYIYM
                     NLDGAISKDKIPNFLEKVNAINSDIKDFYNTETKIKEE"
BASE COUNT          327 a          118 c          189 g          308 t
ORIGIN      
        1 aaatattgta gttttaagaa tgtaattttt tcaggagtga taaacagccc taaccttgaa
       61 ggtccggttt ttgaaaattg ctatttcgat gattgtaaat tcaacaacat acagctttat
      121 gagactaatg atgatactgt ggggtcagga aataaaaaac caataaaagg tatgtttaaa
      181 ggttgtttta tttctaaatg caaaattgaa aattatagat gtgagacgtc taaaatctac
      241 aacgttacac aacctgtaag tattaatgaa aaattaggtt cttatctctt tatgcagtct
      301 tttgttcagg attgcacaat tcagggggga tattgtcctg gttcgagtat acttctgagt
      361 catttttata actgtacgat agcgaaactt gatgctcatg ggatggattt tttggcaaac
      421 gcattcacta agtctagtga taacgaagcc agggcgcagg atcagggaac tgtgtttaat
      481 tattgtaatt tgaatagtat taatatgaag tgtagtcttg gtaatgcgag ttccgatgaa
      541 tataagttta acccagaaga gtatttttct gattacattg aaagaagatc gagaactctt
      601 aagttggatg ataatttttc taactgcgta gggaatatcg agaaggctgt taaaacttca
      661 tttagcaagc agaattcaga gaatgataat atctccttta attattgtga tttgcggagt
      721 gccaatttag acagttatta cagagaagat tggtgcaaag actgttctat tgatccagat
      781 tcaatttatt tagaagatgg tgttacaccg ataaaataca tttatatgaa tctggacgga
      841 gctataagta aagacaaaat acctaatttc ttagaaaagg taaatgctat caatagcgat
      901 ataaaagatt tttataatac tgagacaaag attaaggaag aa
//
LOCUS       DAZGCQ010000358          937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000358 DAZGCQ010000000
VERSION     DAZGCQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.364"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..793)
                     /gene="aqpZ"
                     /locus_tag="ACHK07_005430"
     CDS             complement(98..793)
                     /gene="aqpZ"
                     /locus_tag="ACHK07_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO8105586.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT          301 a          248 c          208 g          180 t
ORIGIN      
        1 gtgactatat gtggcttaac acatcaagga gcgcaacagg aagatgaatc aatatattta
       61 aaaaaggcct gacattgcgc caggccttct gcgttaatta attacgtttt tccagcaggg
      121 tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca
      181 gttgttctaa tgcccagccg ccctggaaga tggcaaccgc ggtgctgcgc gccgggttaa
      241 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
      301 cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc gccgtggatc accaacagga
      361 aacctgcact caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggtg
      421 aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac
      481 cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc tgggcaatta
      541 cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca atagtgaccg
      601 ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc agaacggtca
      661 aaccgaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca
      721 gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat tcagctgcta
      781 attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta
      841 tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa aaacgaaggc
      901 gagtcattca ccagataaat aaatccagta aatttga
//
LOCUS       DAZGCQ010000359          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000359 DAZGCQ010000000
VERSION     DAZGCQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.365"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          274 a          190 c          206 g          266 t
ORIGIN      
        1 ttacaggaac atcacatctg aaatatcctg ttttcgacaa aagataaaca atcacaatca
       61 ataaagcgag tgaagtcatt ctcaaaaaca ttaatttatt tgcatattca atagctcact
      121 gaactccttc acggtaatat gcactaggat ttctgcaata ggtaatcggt gagatgctct
      181 tcctctcttc ctactccttc agagcagaag taaatccagc tatgatgggt aaaaattagc
      241 cagaaaaaat aagagggggc aactaagttg cctggcatgt ttatgacaat aaagagtacc
      301 gtcatgcatc atggcgaaca ggaaattaca gtgccgtctt accggggcga tcagcgcatg
      361 gttgtgtcgt ttaccctggc tcattattac gtgtgcagta agccccggat atcaggtcac
      421 tgagtatatc tttgaatgag cggtaaaccg gggatgcgat ctgctgcccg ggaaagtcga
      481 actgactggt caatagatat caatacgtgg cagccaggcc gtttacaggt atctgacaag
      541 gacaccttct gagcgtgcaa tgtggacggg taatacaacg atggtagcag gctggtcagc
      601 aatcagcagt atatgcccat atttcctgag gccggatatc tgtttcctga gtttgttttc
      661 gtcgtttgct aatattttgt cgaactaatt atcagaacta ttaatgataa cagtgtggca
      721 gggatcttta ccgatatcaa cgtaaataac gaccttaaca ggtgcgtaat tgctggattt
      781 tgccatgctg ttaccctgtt gatacgggtt aattaaataa ctttggggaa gcaggtaccg
      841 gcgttcacta ccactctccc tgataatgta tcctgcatgc ctgataattt ttccgggcta
      901 atgtaaaata accctgaaat atattaagtt gattaa
//
LOCUS       DAZGCQ010000360          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000360 DAZGCQ010000000
VERSION     DAZGCQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.366"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..122
                     /locus_tag="ACHK07_005431"
     CDS             <1..122
                     /locus_tag="ACHK07_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8105587.1"
                     /translation="DNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            133..876
                     /locus_tag="ACHK07_005432"
     CDS             133..876
                     /locus_tag="ACHK07_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8105588.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHR
                     FRCVPHLTVRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            822..>933
                     /locus_tag="ACHK07_005433"
     CDS             822..>933
                     /locus_tag="ACHK07_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8105589.1"
                     /translation="MCLHGDLQRFGRRISLYVNTAAEAIRALSLQVPGFRC"
BASE COUNT          181 a          254 c          293 g          205 t
ORIGIN      
        1 tcgacaaccc taccacggat atccggaagg acagatgcag taaatgcatg cgcgggtgtg
       61 agatgcgcgg catggtggct aatttcggcg gtttcctttc cattaataaa ctttcgcagt
      121 aaatcccgtt ttatgacaca gactgaatca gcgattctgg tgcatgcccg gcggtgtgcg
      181 ccagcggagt cgtgcggctt cgtgataagc accccggagg gcgaacggta ccagccctgc
      241 gtgaatatct ccgcagagcc ggaggcgtat tttcgtattg caccggaaga ctggctgcgg
      301 gcagagatgc agggggagat tgtggcgctg gtccacagtc atcccggtgg tctgccctgg
      361 ctgagcgagg cggaccggag gctgcagata aagagtgccc tgccctggtg gctggtctgc
      421 cggggtgaca ttcaccggtt ccgctgtgtg ccgcacctga ccgtacggcg ctttgaacac
      481 ggtgtgacgg actgttacac cctgttccgg gatgcatacc atctggcggg gataacgctg
      541 ccggattttg tgcgtgagga tgactggtgg cgcaacggcc agaacctgta cctggacaac
      601 ctggcggaca acggctttta ccgggtgtct ccgtcctgtg cacaggcggg cgatatcctg
      661 ctgtgctgct ttggcgcatc ggtgccgaat catgccgcca tttactgtgg caacggtgaa
      721 ctgcttcacc atatacctga acaactgagt aaacgggaga ggtattctga aaaatggcaa
      781 cgacgaacgc attctgtctg gcgtcaccgc cactggtccg catctgcctt cacggggatt
      841 tacaacgatt tggtcgccgc atcagcctgt atgtgaacac ggcagcggag gccatccgtg
      901 ccctgtcgct gcaggtgccg ggattccgct gtc
//
LOCUS       DAZGCQ010000361          930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000361 DAZGCQ010000000
VERSION     DAZGCQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.367"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>930)
                     /locus_tag="ACHK07_005434"
     CDS             complement(<1..>930)
                     /locus_tag="ACHK07_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO8105590.1"
                     /translation="HNIHFDEKAFQSGMLKIQFPNDNEKLSVYLKNKIPFENITIDLS
                     ALEKEALASLKYCNFKKVTFTGAISYENLNGSVFENCFFEDCIFENVSLVSSRRRVKP
                     NNEIPIYGVFKGCFLSHCDINNFNIDTSKIYSVNQDSDVGKPVGAYLFMQSFVYECTL
                     LNGVCKNASVIASSLLCCKIAKLNGAEMDFIGTSFYHDYRREYKYDNHFYYCDFSSSD
                     MTCKRNGIRKDLLDFDPREYFKKDGGKLNLDKRISDFIYINIFPNMSEHHYINDKDVG
                     GDIEKYLKLSNCNLLKTCLGESINGIKLDDCAID"
BASE COUNT          323 a          169 c          120 g          318 t
ORIGIN      
        1 atcaatagca caatcatcca atttaattcc attgattgac tcgcccaaac aagttttaag
       61 taaattgcaa ttactaagtt ttaaatattt ttctatatca ccaccaacgt ctttatcatt
      121 tatataatga tgttcactca tattagggaa aatattaatg tagataaagt cagaaatgcg
      181 tttatccaga ttaagtttac ctccatcctt tttgaaatac tctctgggat caaaatccag
      241 taaatctttg cgaattccat ttcgcttgca tgtcatatca gaggagctaa aatcacaata
      301 ataaaaatga ttatcatatt tatattcacg tctataatcg tgataaaacg aagtccctat
      361 gaaatccatt tcagcaccat taagctttgc tattttacaa cataacaaac tgcttgcaat
      421 aacacttgca tttttacaca ctccattcag aagagtacat tcatatacaa atgattgcat
      481 aaacagatat gcaccaaccg gcttacccac atcggaatct tgatttacag agtatatttt
      541 tgatgtatct atgttaaaat tgtttatatc acaatgagat agaaaacatc ctttaaacac
      601 gccgtaaata ggtatttcat tatttggttt tactcgacgc ctggagctaa caagtgacac
      661 attctcaaaa atacagtcct caaaaaaaca attttcaaat actgaaccat taagattttc
      721 gtaagatata gcaccagtga atgtcacttt tttaaagtta caatatttta gagatgccag
      781 ggcttctttt tccagcgcag ataaatcgat tgtgatattt tcaaagggga ttttattttt
      841 taaatatacg ctaagctttt cattatcatt tgggaactgt attttcaaca ttcctgattg
      901 aaaagccttc tcatcaaaat gaatattatg
//
LOCUS       DAZGCQ010000362          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000362 DAZGCQ010000000
VERSION     DAZGCQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.368"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK07_005435"
     CDS             complement(<1..242)
                     /locus_tag="ACHK07_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105591.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFEL"
     gene            complement(425..733)
                     /locus_tag="ACHK07_005436"
     CDS             complement(425..733)
                     /locus_tag="ACHK07_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105592.1"
                     /translation="MSAEFILSFPDDSWYLRNRNNIVNKILFLSTYRKNDGDMYYLSC
                     DEGEWDYCVRLCIENCSVFIEIFSHPASIEKDLISLFTWIRSQTIISICDNDGEESGW
                     "
     gene            complement(784..>922)
                     /locus_tag="ACHK07_005437"
     CDS             complement(784..>922)
                     /locus_tag="ACHK07_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8105593.1"
                     /translation="SGIRHIAINILTNDTVFKAGLRRKMRKAAMDRNHLASVLAGCGL
                     S"
BASE COUNT          299 a          201 c          168 g          254 t
ORIGIN      
        1 agttcaaacc aggtcaccac acctttcacc cggcgttgaa catcatcacc ctgccatacc
       61 gtcaggtagg ccattttgtc cagtacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagtgaa gagagtgact gggtgagatg aaaggaaacc
      181 accgcaaaag cgtccggtgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct cctgtataag aaaaactaac ctgcactctg ttgtggaaca gcgtgtcagg
      301 gaataatatt aatgagctga tgcccgatat taccggtaat atatcatgta cgtttctgcg
      361 attttattaa taaaaattca attcacatgg ctcatagtat ttctaagagt ttacaaatat
      421 aatttcacca accagattct tctccatcat tatcacagat tgatattatt gtttgacttc
      481 taatccaggt aaataaagaa attaaatctt tttcaataga agcaggatga gaaaaaatct
      541 caataaatac cgaacaattt tctatacaca gtctcacaca gtagtcccat tccccctcgt
      601 cacaagataa ataatacata tctccatcat tttttctgta tgtagacaaa aataatattt
      661 tattaacaat gttattccta tttctcaaat accaagagtc atcaggaaat gaaaggataa
      721 attcagctga catttttaat ctccataatt tcgacctgaa atagttggaa gaccagggca
      781 agattacgaa agcccgcacc ccgcaaggac tgacgcgagg tggtttctgt ccatggctgc
      841 ttttcgcatc ttacgtctta accctgcctt gaataccgta tcattcgtca aaatattaat
      901 agcaatgtgc cgtatccctg aa
//
LOCUS       DAZGCQ010000363          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000363 DAZGCQ010000000
VERSION     DAZGCQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.369"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>905
                     /locus_tag="ACHK07_005438"
     CDS             <1..>905
                     /locus_tag="ACHK07_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8105594.1"
                     /translation="TEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEY
                     GLNVVSGDITDITTPDGRETKFYYNDGNLVTAVVYPDGLESRRAYDERDRLVTETSRS
                     GETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQM
                     TAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYD
                     AWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQR
                     YHYDLTGKLTQSEDEGLVTLWHYDE"
BASE COUNT          217 a          249 c          313 g          126 t
ORIGIN      
        1 tacagaaggc ggggccgggc tgaagcgggt ggtgaaaaaa gaactggcgg acggcagcgt
       61 cacgcacagc gggtatgacg cggcaggaag gctcacggcg cagacggacg cggcgggacg
      121 gcggacagag tacggtctga atgtggtgtc aggcgacatc acggacatca ccacaccgga
      181 cgggcgggag acgaaatttt actataacga cgggaacctg gtgacggcgg tggtgtaccc
      241 ggacgggctg gagagccgcc gggcgtatga tgagcgggac aggctggtaa cggagacatc
      301 gcgcagcggg gagacagtac gctaccgcta tgatgatgcg cacagtgaat taccggccac
      361 aacaacagat gcgacgggca gcacccggca gatgacgtgg agccgctacg ggcagttgct
      421 ggcgttcacc gactgctcgg gctaccagac ccgctatgaa tacgaccgct tcggccagat
      481 gacggcggtc caccgcgagg aaggcatcag cctttaccgc cgctatgaca accgtggccg
      541 gttaacctcg gtgaaagacg cacagggccg tgaaacgcgg tatgaataca acgccgcagg
      601 cgacctgact gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg
      661 gggaaaggcg gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc
      721 cggacgtgtc atcagcctga ccaacgagaa cggcagtcac agcgacttca gctacgatgc
      781 gctggaccgc ctggtacagc agggcggctt tgacgggcgg acgcagcgtt atcattatga
      841 cctgaccgga aaactcacac agagtgagga tgagggactt gtcaccctct ggcactacga
      901 cgaat
//
LOCUS       DAZGCQ010000364          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000364 DAZGCQ010000000
VERSION     DAZGCQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.370"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          257 a          226 c          177 g          240 t
ORIGIN      
        1 gtcagcatgt cttcatcctc atcagcagct cttcagtgga taacatccac agttacacct
       61 gcatttacgc gcaacaaaaa caccagatga agattttatt cattcagctc cgctaaaaat
      121 taatcttgcc gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact
      181 cacaatccct tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg
      241 ttcgtatacc ctgttcgatg gattattatt gatccttatg atcgcttcac gtaaacacga
      301 ttctggctcc acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg
      361 tcacagacgg tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa
      421 actctctatg cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca
      481 agcgaatgac tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat
      541 acactcagac tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcccctttat
      601 cttcttatgg cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc
      661 cccggtaaga aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg
      721 catattttca tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt
      781 aaagaatgct ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc
      841 ctctggagaa aagtgctact tcttgtcgat tcagactagt tcaggaaatt atacgaaaat
//
LOCUS       DAZGCQ010000365          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000365 DAZGCQ010000000
VERSION     DAZGCQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.371"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..281)
                     /locus_tag="ACHK07_005439"
     CDS             complement(<1..281)
                     /locus_tag="ACHK07_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105595.1"
                     /translation="MKVFHNIFKHISSNHKDKYSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIK"
     gene            664..>864
                     /locus_tag="ACHK07_005440"
     CDS             664..>864
                     /locus_tag="ACHK07_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO8105596.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRG"
BASE COUNT          260 a          152 c          154 g          298 t
ORIGIN      
        1 tctttaatat ctatagatgg ctcactattg taaaaccata ttttttctat ctgaggacta
       61 tgttcagtta cgttccagca gccatgttgc aggaaataga tgatatgcag gccaaaatcg
      121 ttatctattt gcgaatgttt atccagctta tcaatttcaa ctatctctga acgatgtttt
      181 ttatcaacct tgccatggtg ttgatgatta ttaactttat ctgaatactt atctttatgg
      241 tttgaagaga tgtgcttaaa tatattgtga aatactttca taaataaccc gtgatatttt
      301 tgacttgtta gtaacaaaat aaataataca tgtcttaaag acatattttc gacgatataa
      361 agcaataatg cgtatatgct cgctgatttt aagcaccgag accatccggt cttttaccaa
      421 ataatgaaaa agaatccctc cctggaagac aattgtaaaa aacaacagaa taagaattaa
      481 tcttaggata aatatttatt tatcatggcc ttttgaacgc tgaaaagtag cgctctgcaa
      541 agtaaacccc gctatagtta tgctatgcat ttgtctttaa tgatatctcg tcattgtatt
      601 aacaggctga aattatcatt aatgcacatt tcaatattag cagggatacc tgaggagttt
      661 aaagtgaaca tatatatcgg gtggcttttc aaattaatcc ctttaattat gggcttgatt
      721 tgtatcgcgt taggtggctt tgtgctggaa agttcagggc aaagcgagta tttcgtcgcg
      781 ggtcatgtgc tcatttctct ggcagccata tgcctggcat tattcactac cgcatttatt
      841 atcatttcgc agctcacgcg cggc
//
LOCUS       DAZGCQ010000366          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000366 DAZGCQ010000000
VERSION     DAZGCQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.372"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..689
                     /locus_tag="ACHK07_005441"
     CDS             <1..689
                     /locus_tag="ACHK07_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFO8105597.1"
                     /translation="MINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTF
                     SLTPEGRLTARNADISGAVTANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAF
                     PRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKC
                     NGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVM
                     KKSTAGISIS"
BASE COUNT          202 a          203 c          261 g          183 t
ORIGIN      
        1 cgatgattaa cccggagaac ggcaacacca cgccgctgtt tgtggcgcag gggaatcagc
       61 tgttcatgaa cgatgtgttc ctgaagcgac tgtttgcagt gagcatcacc tcgtccggca
      121 atcccccgac gttctccctg acgccggagg gcaggctgac ggcccgcaat gcggacatca
      181 gtggagctgt cacagcgaat agcggcacgc tcaataatgt gaccattaac cagaactgcc
      241 ggatacttgg aaaactgtct gccaaccaga ttgaaggcga tattgttaaa accgtgggga
      301 aggctttccc gcgggactcc cgcgcaccgg agcggtggcc atcggggacc attaccgtca
      361 ggatttatga cgatcagcct tttgaccggc aaatagtgat cccggcagtg gctttcagcg
      421 gcgctaaaca tgagagagag cataacgata tttattcatc atgccggctg atggtgaaat
      481 gtaacggcag ggtgcttttt gaaaggacgg cgctggctga gtctcaggtg tattcagggg
      541 tgattgatat gcctgcagga agggggcata tgacgctgga gttttctgtg tctgcctggc
      601 tggtgaatgg atggtacccg acagcgagta tcagcgatct gctggttgtc gtgatgaaga
      661 agtccacggc gggtatcagt atttcgtgat catgaggagc cggattgccg gaaggagaca
      721 aaaaccgtac agtatgcgcg ggtgcctttg gctgatggcc ggagggaaca cctgaaggcc
      781 ggatgtgaaa aggccccgga caaacattca tgtttaaccc gaggcctgac cattcatcct
      841 aagcaagtg
//
LOCUS       DAZGCQ010000367          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000367 DAZGCQ010000000
VERSION     DAZGCQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.373"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>843
                     /locus_tag="ACHK07_005442"
     rRNA            <1..>843
                     /locus_tag="ACHK07_005442"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          214 a          191 c          278 g          160 t
ORIGIN      
        1 gatggagggg gataactact ggaaacggta gctaataccg cataacgtcg caagaccaaa
       61 gagggggacc ttcgggcctc ttgccatcgg atgtgcccag atgggattag ctagtaggtg
      121 gggtaacggc tcacctaggc gacgatccct agctggtctg agaggatgac cagccacact
      181 ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat tgcacaatgg
      241 gcgcaagcct gatgcagcca tgccgcgtgt atgaagaagg ccttcgggtt gtaaagtact
      301 ttcagcgggg aggaagggag taaagttaat acctttgctc attgacgtta cccgcagaag
      361 aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcaa gcgttaatcg
      421 gaattactgg gcgtaaagcg cacgcaggcg gtttgttaag tcagatgtga aatccccggg
      481 ctcaacctgg gaactgcatc tgatactggc aagcttgagt ctcgtagagg ggggtagaat
      541 tccaggtgta gcggtgaaat gcgtagagat ctggaggaat accggtggcg aaggcggccc
      601 cctggacgaa gactgacgct caggtgcgaa agcgtgggga gcaaacagga ttagataccc
      661 tggtagtcca cgccgtaaac gatgtcgact tggaggttgt gcccttgagg cgtggcttcc
      721 ggagctaacg cgttaagtcg accgcctggg gagtacggcc gcaaggttaa aactcaaatg
      781 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgatgc aacgcgaaga
      841 acc
//
LOCUS       DAZGCQ010000368          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000368 DAZGCQ010000000
VERSION     DAZGCQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.374"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..105
                     /locus_tag="ACHK07_005443"
     CDS             1..105
                     /locus_tag="ACHK07_005443"
                     /inference="COORDINATES: protein motif:HMM:NF016846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8105598.1"
                     /translation="MDDLEKIEFDVLNFIWRIDGKDVLPDRTRSMLGF"
     gene            153..542
                     /locus_tag="ACHK07_005444"
     CDS             153..542
                     /locus_tag="ACHK07_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105599.1"
                     /translation="MDKETEMTTDTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNVADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
     gene            563..700
                     /locus_tag="ACHK07_005445"
     CDS             563..700
                     /locus_tag="ACHK07_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO8105600.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
BASE COUNT          184 a          207 c          258 g          193 t
ORIGIN      
        1 gtggacgatc tggaaaaaat cgaatttgat gtgctgaatt ttatctggcg cattgacggt
       61 aaggatgtgc tgccggatcg cacccgctcg atgctgggct tctgattatt gtttaacaga
      121 gaacggcggg gatcccgccg tggagactga aaatggataa agaaacggaa atgaccactg
      181 acactgccgt cagtaacggg gaggcttccg cggtggcggt tgccgctacg gctgcaccta
      241 agaacgtggt gaagctgacg gtgcctgttg tgcgcggaga tgtgtcgtac agtgaaatta
      301 aaatcggtcc gctggcgaat caggccggat cgctgcgtgg gctgtctttg cttgcgcttt
      361 ataatatgaa ctgtgatgcc atggtcacct ggcttgcgcg tgtgacagag ccaaaactca
      421 aggaggccga aatgtatgcc atgaatgtgg cggattttac ggcgctgagc tcggcggcgg
      481 ttaatttttt cgcaccctcg gggtcgaatg caccggcacc ggagaacacg gcagagccgt
      541 aacctgtctt tgctttgact gcattgaaga tcccgttgcg gatattgctg ttatttttaa
      601 ctggccgccc gcagagatat ttgcaatgac gcctgcagag gtggtcgcct ggcgtgagcg
      661 ggcggctgcc agaagcggaa gtcacaatca tgatgaataa tctgcatatc agggttgctc
      721 tggcggcggc agacaccctg acacaacctc tcaggcgcgc ccgcgaaacc gtgggcgcgc
      781 tttctgattc cctccgtgaa actcaggact ccatccgctc gcttgaacgg cagagtgaag
      841 cc
//
LOCUS       DAZGCQ010000369          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000369 DAZGCQ010000000
VERSION     DAZGCQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.375"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..>822
                     /gene="reiD"
                     /locus_tag="ACHK07_005446"
     CDS             69..>822
                     /gene="reiD"
                     /locus_tag="ACHK07_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO8105601.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKITSIAYRVGFSNLSHFQNYFKN"
BASE COUNT          238 a          149 c          193 g          242 t
ORIGIN      
        1 ataatggagg aaaaatcatg cgtttttgct tctgtccttc agaacaagat aataagtccc
       61 gggctcagtt ggcgtatgtc agtcaggtta gcgaaagcaa tggcccgttt gtacggttca
      121 tgcatatgca tcccaggacc gtggagctaa tccttatcac cgagggaaag ggggagtatt
      181 tcatcggtga tcgcatttac ccagtcagaa aaggtgattt ggttatttat aacagcgaag
      241 tagtgcatga tgagtatctt gaaagcggac ggcctgtggg tactatttgc tgtgggataa
      301 acaatattgt gtgcccgggt ctgcgggaga atgctctgat tccagatgat actgtgcccg
      361 taattccact atatcatcat tatgcgacga tcgagaaatt gatgagttca atatttaatg
      421 ttataaatca gcatacagta gaaggaccaa caatggcgca gttgctgacc agggtgctgc
      481 ttaaatacgt cgagggaaat gtattaatgc gcacaggtaa tgatataaca cggcgccata
      541 aaaatttact ggattcaata aaagcttata ttgaccataa tttctatgaa cccatccgtc
      601 tggatgctct ggcgactaag tttaatgtca gcccttacta tgtttctcat gaatttaaac
      661 ggcgctatgg atattcgcca atggactatt tgattaaacg tcggttaggg gaggcacaat
      721 ctttacttac cactgatgaa ggtgggtgtg aaaaaattac ctcaattgcc tatcgtgttg
      781 gtttcagtaa tttgagtcat tttcagaact attttaaaaa ta
//
LOCUS       DAZGCQ010000370          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000370 DAZGCQ010000000
VERSION     DAZGCQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.376"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..770
                     /locus_tag="ACHK07_005447"
     CDS             54..770
                     /locus_tag="ACHK07_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8105602.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          272 a          139 c          171 g          233 t
ORIGIN      
        1 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
       61 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
      121 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
      181 taaacacatc tttaaaattc gttattgaac gtgcaaaaca ggcacaagtt ccaaagcacg
      241 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
      301 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
      361 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
      421 cggcaggttc tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag
      481 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
      541 aagaagaagg taacattgtt atttatactg aacctactga ccttcataaa ggaatcgcgg
      601 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
      661 cagaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
      721 aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta
      781 aagaaatctg tctttatggc agatttcttt aatct
//
LOCUS       DAZGCQ010000371          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000371 DAZGCQ010000000
VERSION     DAZGCQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.377"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..302)
                     /locus_tag="ACHK07_005448"
     CDS             complement(<1..302)
                     /locus_tag="ACHK07_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8105603.1"
                     /translation="MQIIIMTRDRYLEYGLMCVLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRCGAWLFGHTARPLTR"
     gene            complement(365..571)
                     /locus_tag="ACHK07_005449"
     CDS             complement(365..571)
                     /locus_tag="ACHK07_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105604.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(699..>815)
                     /locus_tag="ACHK07_005450"
     CDS             complement(699..>815)
                     /locus_tag="ACHK07_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8105605.1"
                     /translation="QAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          216 a          261 c          165 g          173 t
ORIGIN      
        1 cgggtcagtg gtcttgccgt atgtccgaac agccacgccc cacaacggat ggcctgcctg
       61 atatctgtca ggcatctcac agaggaaaca ggaatgacaa gaaaacgacg cccacagaac
      121 atacagtatg taagcctttc cagattacgg tcacagagaa tcacataact gtcttcggga
      181 agtggcagac ggcgctttcc ggcatcaaac agttcacggc ctgtcgtgag cctgtatccg
      241 ttcagcacac acatcaggcc atattccaga tatctgtcac gggtcataat aataatctgc
      301 atactctcag acccttatct cccggatacc ggctccgttt accattaccg gtataactga
      361 ctcatcattc ataacggaaa ctgtctgccg catacatcag tgcccgctct tccagctctg
      421 acagactcag ctctttcagt tccccttctc cttcagcaag aagacaacat tcaaaaacag
      481 ctgcatcgtc gcattccaga cgaacctgca ccctgtcagc ccgaccgttt acagtaaata
      541 cctgcactga acttatgcga tagttcagca tatacactct ccctctcagc tcctcaggaa
      601 taccccggaa ccgtcagtca tgccaccggg gagaaaagca ggcagcgaat catgaccgca
      661 tacagaacgc tgcccgcccc ggtgactcag acccgggttc agaactccca gttgacaccc
      721 acattccagg aaacatcacc gtcactgaac gcagtacctg cctttgccgc agcctgttcg
      781 ttaaagcgat atcccatgcc caccgcaaca gcctg
//
LOCUS       DAZGCQ010000372          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000372 DAZGCQ010000000
VERSION     DAZGCQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.378"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..715
                     /gene="ydeO"
                     /locus_tag="ACHK07_005451"
     CDS             <1..715
                     /gene="ydeO"
                     /locus_tag="ACHK07_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO8105606.1"
                     /translation="NILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
                     YLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIF
                     AACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQT
                     TFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEY
                     RCQRHTGMNTDNTMSALAI"
BASE COUNT          252 a          161 c          164 g          238 t
ORIGIN      
        1 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
       61 tgttcgtaca cattttgaac cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa
      121 taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc
      181 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
      241 caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc
      301 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
      361 cgggaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
      421 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac
      481 gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt
      541 agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat
      601 ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
      661 tcaaaggcac accggtatga acacggacaa cacgatgagt gctttagcta tttgattatt
      721 tgctaacggg tagtcaacca cacacgctac gtaagaatta aatggggcag ccattccttg
      781 ccccgcgttg tttttaggcg atatatttat tgaaa
//
LOCUS       DAZGCQ010000373          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000373 DAZGCQ010000000
VERSION     DAZGCQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.379"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            320..>810
                     /locus_tag="ACHK07_005452"
     CDS             320..>810
                     /locus_tag="ACHK07_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105607.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPEL"
BASE COUNT          291 a          149 c          125 g          245 t
ORIGIN      
        1 ataaaaggcg tcaattatcc atgctccctc acgaaacaga agctgaactc ttaacacctg
       61 ctatcggaac tcactgaata aacaactaaa aaggacattg tgaaaattaa catcaacagt
      121 atgtaaagtg atttgttttg tcttaaatgg gtgaatgtaa aaaaatattc tatacaatcc
      181 acacatcctt gtatcatata tcaagtaaac atccacagag ataacagctt atttcaaata
      241 agcagattct gtttgatact tcagaaaaat agtatatgct cctgatttac tgatgttata
      301 agttatacag gatagttaaa tgtgtgattt tataaaatca tgttatgcag caccaagaat
      361 taacaaaatg gctgattttt tctcatcaac agcatctgga gtaaaacata aacctttgaa
      421 gcatgtaaaa attgataaca ggcttttccc atctcctgag tatataattt tttctgacta
      481 tcttacgaaa aacagtggtc catttgttaa ccactacttt tgcagtattc catacagcaa
      541 agaagaagaa tgcaggctgg gtgtgactat tatgaaatat gcaagccatc gaaataagcc
      601 attgagctta tggtgtcttg gaatggcaga aggagccatg gccagaacaa tttctcaact
      661 atcaaaagga caggttttat cattaacaac cagcccaaca aaagaaaatg aacctgtatt
      721 ctttagtcat ggttctcata ttcactctta ctttatttgc tccccattct ttcttgttca
      781 tgaagaaata aaaaataaca accctgaact
//
LOCUS       DAZGCQ010000374          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000374 DAZGCQ010000000
VERSION     DAZGCQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.380"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..132)
                     /locus_tag="ACHK07_005453"
     CDS             complement(<1..132)
                     /locus_tag="ACHK07_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO8105608.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     "
     gene            complement(158..>803)
                     /locus_tag="ACHK07_005454"
     CDS             complement(158..>803)
                     /locus_tag="ACHK07_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8105609.1"
                     /translation="HEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQN
                     RASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLN
                     VLLNGKVKFISGKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSR
                     SFLIAKASSPWCKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF"
BASE COUNT          265 a          146 c          108 g          284 t
ORIGIN      
        1 atcatcaata gaacgaaaaa cttttaatgt tttaaccctg caagttaaaa agtcattcag
       61 aacttgctca taaaagggat cattatcttt tatgaaaact ttcaacccat ttatcgtttt
      121 attatatatc atattgaatt attttaacct taaatcttta aaagcatttt tctttataat
      181 actttaaata attaaaaatg cctgccatat aatgcttttg tttaaattta tgttttgcac
      241 aataccgata ctgattgcta tttacaggtt taagtaaatc ttctttacac cacggagaag
      301 atgctttggc gatcaaaaaa ctacgtgaca ccggatagtc agcccattca tgccagggtt
      361 ttgtaggtcc cacatagtgt ataaacacgg tatcatcatt gactggatta tcaactttgt
      421 cttttaattc atagttaata ctatatcggg tattatattt cccagaaata aacttcactt
      481 taccattcaa taatacattc agtacatctt gatcaaggtg ggtgatttta cttacccaat
      541 ctggatcacg caacatttca atagcttttg acgaaatgtt attaagattc cactcatcaa
      601 tatttatcag taaaaaacca gcattgaaat atccagaagc taattgtggt gtagttaata
      661 ccgaggctcg attctgccac cattcaacat ctctttcagc tacaactgcg gcaatttcat
      721 tagtagagaa ttgataatct aagagttctt taatactacc cttgcaagca atatctgcat
      781 caagatatag tattttttca tgt
//
LOCUS       DAZGCQ010000375          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000375 DAZGCQ010000000
VERSION     DAZGCQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.381"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..>802
                     /locus_tag="ACHK07_005455"
     CDS             50..>802
                     /locus_tag="ACHK07_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO8105610.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDISFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVN"
BASE COUNT          209 a          190 c          232 g          171 t
ORIGIN      
        1 tccggtggtg tttctggtat gcaactgtgg cgggaaaaac ggaggaatta tggtattgct
       61 gaacggtaac acggtttatt cccccctgaa agaactggtg aaaatgcctg atttcagcgt
      121 ttcactggac agggagcagg ataaaaacac acttcaggaa ctgaataacc gggtgatatc
      181 gctttccatg acagacaacc gggggtttga agcagacagg gtcactcttg tgctggatga
      241 tgccgacggg cagctggctc tccccgggcg gggaacacac atcagagtca tgctgggatg
      301 gaagggagag cctctggttg ataaaggcac ctacattgtt gatgaggtcg ctcatgaagg
      361 acctccggat cggctgacac tgagtgcaaa cagcgcagac ttcagggagg aatttaacgt
      421 caaacgggaa gtatcctggc acgacgtgac tgttgaacgc gtggtttccg ctattgccca
      481 ccgttacgga ctgaaagccc agataagcga tatgcttatg gatattgaaa tcgatcatgc
      541 cgaccagacg gaagaaagcg acatatcatt ccttacccgc atggctgata tgctgggagc
      601 catcactacc gtcaaaaatg gctgtctgct gttcattctg ccgggtggcg gcgtgacagc
      661 tgacgggcgc ccactcccct catttgcact ggcacgcagt gacggagaca gtcaccagtt
      721 cagaatagcc gatcgccagg catacacagg ggtgaaggcg tactggctgg atttgaatca
      781 tggcaaaaag aaagccgtca ac
//
LOCUS       DAZGCQ010000376          798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000376 DAZGCQ010000000
VERSION     DAZGCQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.382"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(212..364)
                     /locus_tag="ACHK07_005456"
     CDS             complement(212..364)
                     /locus_tag="ACHK07_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105611.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            382..573
                     /locus_tag="ACHK07_005457"
     CDS             382..573
                     /locus_tag="ACHK07_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO8105612.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          232 a          171 c          127 g          268 t
ORIGIN      
        1 tatttttata taagttgcat tcagtttcat agatgctcag cagaatcccc cacatcctga
       61 aggaggtgta ttcagacagg catcccacct gacttcgaat gatgattatt catcactata
      121 gagagcattg attctaagtg tcatatgaaa gttccaattg atatatatca aacaaaataa
      181 ccctgattaa tgaattatta cgtttatcat gttaattcat cattattaca tcatcattgt
      241 aaataattaa attaacttcc ataacattaa aatatgtatc cactgacgct tttttacata
      301 acgaagaatt gaccattttg tcctgttgtg ccttaatgta agtaccgtcc acagcgtggg
      361 acatacttca aggaaccttt tgtgagtcag gcaaccagta tgcgaaaacg acaccgattt
      421 aacagtcgca tgacccgtat cgtactgctc atcagcttta tcttcttctt tggccgtttt
      481 atctactcgt ccgtcggtgc ctggcagcac catcagagca aaaaagaagc tcagcaatcc
      541 acactctccg tcgaatcacc ggtacaacgt tagcggttac cttctccact ttcacagaac
      601 ataacggcgc ttcgctgtcg gatgcttttg ctctttggaa ttatcaaagc ggcagatatt
      661 ctttcatctt aaattttacg tctttgtcct gactgatgtt tatcctgttt ggctgcaaaa
      721 ttaatatata tgttgtaatg atgtgttttc ataaattatt ccccaagtga attttaataa
      781 atttaatcta tcccttta
//
LOCUS       DAZGCQ010000377          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000377 DAZGCQ010000000
VERSION     DAZGCQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.383"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHK07_005458"
     CDS             complement(<1..221)
                     /locus_tag="ACHK07_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO8105613.1"
                     /translation="MERSFRLDLMRLLGLLLIIFAHIGPPNALFQIRTFDVPMMIFVS
                     GVAYYISSPSFFSWKIYYIKRVSRLLFPV"
     gene            complement(227..>784)
                     /locus_tag="ACHK07_005459"
     CDS             complement(227..>784)
                     /locus_tag="ACHK07_005459"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8105614.1"
                     /translation="LNYNHFMRNSRSNIYLAVGRLEQQKGFDRLLKIWCTFYKNNESA
                     KLFIAGEGSQKDKLHCLCKELQISQSVIFLGKVDNIDRYYKKADVLLMTSYYEGMPLV
                     LLEAKSWSLPVIAYDCPTGPREIIANNIDGFLIPDNDHELFISKMIELTSNDTLYYEF
                     CKNTAITSRSFNANAACQKWNNLIE"
BASE COUNT          284 a          143 c          100 g          257 t
ORIGIN      
        1 caaacaggaa ataataatct tgaaactctc ttgatataat agatcttcca agaaaaaaaa
       61 gagggactcg atatataata agctactcca gaaacaaaaa tcatcattgg tacatcaaat
      121 gttcttatct gaaataaggc attaggtggc cctatatgag caaaaattat taacaacagc
      181 ccaagtaatc tcattaaatc cagacgaaaa cttctttcca ttattttcac tctattaaat
      241 tgttccattt ttgacaagca gcatttgcat taaaacttct agatgttata gctgtgtttt
      301 tacaaaactc ataataaagg gtatcattag aggttaactc aatcattttt gaaataaata
      361 actcgtgatc attatctggt ataaggaaac catcaatatt attcgcaatt atttctcgtg
      421 gcccagtagg acaatcataa gcaataacag gtaatgacca cgatttggcc tctaataaaa
      481 ccaaaggcat tccttcataa tatgaagtca ttaataacac atcagctttc ttgtagtatc
      541 tatcaatatt atcaacttta ccaagaaaaa ttacagattg tgatatctgt aattctttgc
      601 acaaacaatg taatttatcc ttttgactac cttcacctgc aataaaaagt ttagcacttt
      661 cattattctt ataaaaggta caccatattt ttaataacct gtcaaatccc ttttgttgct
      721 ctaatctacc gaccgccaag taaatgttac ttctggaatt tcgcatgaag tgattgtaat
      781 ttaa
//
LOCUS       DAZGCQ010000378          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000378 DAZGCQ010000000
VERSION     DAZGCQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.384"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..249
                     /locus_tag="ACHK07_005460"
     tRNA            174..249
                     /locus_tag="ACHK07_005460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Ile,seq:cat)
BASE COUNT          227 a          148 c          177 g          226 t
ORIGIN      
        1 aacatttgct tgttgactgc gagtatcgac tttgcgaatg gaggcgatgt cgggttttat
       61 gtcgcatgta ttgacgaccc aggatggatg agaacgcata aaaatctgtt atttacatca
      121 taagatcatc atgtttattg cgtggtttat aagtattgtt attgcatact taaggccctt
      181 tagctcagtg gttagagcag gcgactcata atcgcttggt cgctggttca aatccagcag
      241 gggccaccaa atcatcattc atgtatcagg aagagttaag ggtacaaggg gtactcgatg
      301 gcggtctata tttcatgact aacgccattt aaaggaagaa gtgcggtggg tggtttcgaa
      361 tgagggctgg tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca
      421 ttcaaaagta cctggaaaaa atgcccgcta tgccaagcgg gcaaattgct tacatgtgga
      481 attaagtaat taacgataaa gccagagtat tatcatttat tatacccatt catttaatca
      541 ggttaattac tcaaatcact gaactattct ctgttcgctc actgatgatg agtgagtacc
      601 acaaccaatg tatgtagaac aatgccgtag agtaaggatg aaatgtctct gccacgcatt
      661 aaacatctac tactgaccag aacacccgcc gcaggtagat gagttttgtg agcgttacag
      721 gagcgtggct atgctcatta accaacgcta ttgagatata aatttctaac acttgaaa
//
LOCUS       DAZGCQ010000379          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000379 DAZGCQ010000000
VERSION     DAZGCQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.385"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>778)
                     /locus_tag="ACHK07_005461"
     CDS             complement(<1..>778)
                     /locus_tag="ACHK07_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105615.1"
                     /translation="TVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGD
                     LKNALRDSSAALMNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLA
                     DNIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNV
                     SQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYE
                     IDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKLTMTP"
BASE COUNT          163 a          238 c          182 g          195 t
ORIGIN      
        1 ggcgtcattg tcagtttccc ggtcgcagca tcttcggtct gtgtgtaaaa acgaccacca
       61 atattgacaa gcgggttccc ctggctgtcg gtgtactcca ccggaatccc cgtcacatca
      121 atctcatagc tgtaatttcc cttatcatcc ttactgatgg ctgaaacccg ggtattctgt
      181 ccgtggcgga cttcaactgt ctgggaagaa acagcaacag cctcatcacc ggattcagtc
      241 ttcagtgccc aggtcgttgc tgccgttgcc tgactgattt cacggttcag ttgtgaaacg
      301 ttaacggcgt cctggtcatt caccccgtct ttcacgccgg aaataacccg gccagcagca
      361 tcaataccgt tcacagtcac tgacgggcca tccttgatgg tcagcccgtt tttatccatc
      421 cgggttccgc cgacagaaac actgccgtca tctgccaggc gaatgttatc agcgagtctg
      481 accgtcaggc tcccgttctg aacagtcgtg ctgatattct gcccgtcacc accaaccgtg
      541 atatgcttcc cgagagacgt gttcaccttg cttccgtcat ccgcctgcag accaaagccg
      601 gtattcatca gtgccgcact gctgtcacgg agagcatttt tcaggtcacc gatattggcc
      661 gcattattca gaacatctcc accggcgtca gcaagcgcag tgccgttcag accactggcc
      721 acactggtta tctgatttcc accgttattc aggccggagc cggtaaggga aaccgtat
//
LOCUS       DAZGCQ010000380          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000380 DAZGCQ010000000
VERSION     DAZGCQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.386"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>778)
                     /locus_tag="ACHK07_005462"
     CDS             complement(<1..>778)
                     /locus_tag="ACHK07_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105616.1"
                     /translation="TVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGD
                     LKNALRDSSAALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLA
                     DNIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNV
                     SQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYE
                     IDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKLTMTP"
BASE COUNT          162 a          239 c          183 g          194 t
ORIGIN      
        1 ggcgtcattg tcagtttccc ggtcgcagcg tcttcggtct gtgtgtaaaa acgaccacca
       61 atattgacaa gcgggttccc ctggctgtcg gtgtactcca ccggaatccc cgtcacatca
      121 atctcatagc tgtaatttcc cttatcatcc ttactgatgg ctgaaacccg ggtattctgt
      181 ccgtgacgga cttcaaccgt ctgggaggaa acagcaacag cctcatcacc ggattcagtc
      241 ttcagtgccc aggtcgttgc tgccgttgcc tgactgattt cacggttcag ttgtgaaacg
      301 ttaacggcgt cctggtcatt caccccgtct ttcacgccgg aaataacccg gccagcagca
      361 tcaataccgt tcacagtcac tgacgggcca tccttgatgg tcagtccgtt tttatccatc
      421 cgggttccgc cgacagaaac actgccgtca tctgccaggc gaatgttatc agcgagtctg
      481 accgtcaggc tcccgttctg aacagtcgtg ctgatattct gcccgtcacc accaaccgtg
      541 atatgcttcc cgagagacgt gttcaccttg cttccgtcat ccgcctgcag accaaagccg
      601 gtattcacca gtgccgcact gctgtcacgg agagcatttt tcaggtcacc gatattggcc
      661 gcattattca gaacatctcc accggcgtca gcaagcgcag tgccgttcag accactggcc
      721 acactggtta tctgatttcc accgttattc aggccggagc cggtaaggga aaccgtat
//
LOCUS       DAZGCQ010000381          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000381 DAZGCQ010000000
VERSION     DAZGCQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.387"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            195..437
                     /locus_tag="ACHK07_005463"
     CDS             195..437
                     /locus_tag="ACHK07_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105617.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(485..>778)
                     /locus_tag="ACHK07_005464"
     CDS             complement(485..>778)
                     /locus_tag="ACHK07_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO8105618.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT          242 a          190 c          135 g          211 t
ORIGIN      
        1 ttcatctctc ttcactcatg ttataacata acaataagat acattctcag ctagatcatt
       61 tcaacagaat ccccgagccc aagggtaatg tggcatcgca gcctctcatc aaatggaaga
      121 catgtgattg cacatttatc atacaagacg tatgataaca ctgtgttatt tatcctgctg
      181 ccgagggtat agatatgaat aacgaatctg aaaaagcaat gtggcgttgt aagcctgtgg
      241 ataatccatg gacgttggat tcattactaa aaaagactat ttctgctgtt ttacctgcat
      301 cagacaaagt ttcacaccaa aataaaaaca aattacgtaa taatgaattc attaaaattg
      361 agcatccatg ctcaaagtct gaatccagaa tcgatggtga ctacatcatt agtggaggaa
      421 cagaataccg tttgtaatta agcaggagaa ttttctgtaa attctcctgt aactttttta
      481 cttcctattt tatacctcga ttagcaatac tccctgcact cgaagctgca cctttcgctt
      541 ccgaaatacc attcggtaat cctcgactga ttccatcgcc aatatgaaca ccagcccagg
      601 acaacgctcc cagccacact gccggcaaga cgagaaacat cgtccccatc accagattca
      661 ttatcagatc atccgatgtg ttctggaatc ccatcatatt gaaccgggta tgtgtatccg
      721 aactgtacag cgccgtcagc atccagctgt caagccaccg tgcaagctcc caccagaa
//
LOCUS       DAZGCQ010000382          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000382 DAZGCQ010000000
VERSION     DAZGCQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.388"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..>774
                     /gene="espX1"
                     /locus_tag="ACHK07_005465"
     CDS             72..>774
                     /gene="espX1"
                     /locus_tag="ACHK07_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO8105619.1"
                     /translation="MTDGISISPHCLYKSNIVDDVIINKTKQNELVKVFCEYKTEFLI
                     LFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYRCKLIEHTVQFYFFKHKGITLLRLD
                     VFDDRTSECLSEEIKTYQECHEKFVKFLKANFNQEIYPQLYTPEIFYEACRNLQSFYD
                     HQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGMMWQ
                     NFKTSNVAYSQLCEKL"
BASE COUNT          300 a          125 c          111 g          238 t
ORIGIN      
        1 cgatttttga tagtagtcac attcactcat aaattaaatt aataaaaatt catcaacttc
       61 aaggactgat aatgacagat ggtatctcaa tttcgccaca ttgtctttat aaatcaaata
      121 tagtagatga tgtaataata aataaaacaa aacaaaatga gttagtaaaa gtgttttgcg
      181 agtataaaac agagttctta attttgtttg atgacttttt cagaagtcag gatttaccta
      241 aaccatctcc agtccttcat catttttttc aatatactca tctgagagat gcacattttt
      301 atagatgtaa actcatagaa catacagttc aattttattt ttttaaacac aaaggcatca
      361 cattacttag attggatgtt tttgatgaca gaacaagcga atgtctttct gaagagatta
      421 agacatatca agaatgccat gaaaaattcg ttaagttttt aaaggcaaac ttcaaccagg
      481 aaatataccc acagctatat actcctgaaa tattttatga ggcctgcaga aatctccagt
      541 cattttatga tcatcaggag acgtcacaaa acgctaaata tagtgcaata gacaagaaaa
      601 aaagtcatct taaccaggaa atcaaaaagc taattaaaaa aaatattttc cctgagctat
      661 acaatgagca atgcaataaa atcccctcct catcgataga tgataaccag ggaatgatgt
      721 ggcagaattt taaaactagc aatgttgcat attcacaact atgtgaaaaa ttat
//
LOCUS       DAZGCQ010000383          772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000383 DAZGCQ010000000
VERSION     DAZGCQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.389"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>772
                     /locus_tag="ACHK07_005466"
     CDS             <1..>772
                     /locus_tag="ACHK07_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105620.1"
                     /translation="LNPKYCGATVRDASDYLVYRFFAAVRRTIDKAGLGWYGVRTVEP
                     HHDGTPHWHMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPTKGTP
                     TSYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQF
                     FGIPPRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLI
                     PRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPSLGERY"
BASE COUNT          180 a          253 c          207 g          132 t
ORIGIN      
        1 ctgaacccga aatactgcgg agcaacggta cgcgatgcca gtgattatct ggtttaccgc
       61 tttttcgccg ccgtccgcag gaccatcgac aaggccgggc tgggctggta cggcgtgcgc
      121 accgtggagc cccaccacga cggtacaccg cactggcaca tgctggtatt caccagccag
      181 gaaaacgaag cccgtatcac tgaaatcatg cgcaacgccg ccatcaggga ggaccgcgca
      241 gagctgggtg atgatatctc cccgcgtttt aaatgcgaaa aaatcgaccc gacaaaaggc
      301 acaccgacaa gctatatcgc gacctatatc ggtaagaatc tggacgccag cgccttcatg
      361 gggaatgacc cgaaaaccgg caaaccttac gtcgataagg aatcaggcaa aaccatggcc
      421 gaaaccgtgg agaacgccat cggctgggca ggccttcacc gcatccacca gttccagttc
      481 ttcggtatcc ctccccgcca ggtatggcgc gaactccgcc gtcttgccgg gcaaatggcc
      541 cgtaacccgg ctgccccgca gcgtctggac catgacgaca ttgacgccat actggccgcc
      601 gccgacgtgg gatgttttgc cacctatatc acccgccagg gtggtgtgct cattccccgc
      661 aacacgtacc tggtccgcac cgcatacgaa actgccgaag aggccaacga ttacggcgaa
      721 ttcccgcaac gtatttacgg cgtccgcgcc ccgtcactcg gggagcgcta ca
//
LOCUS       DAZGCQ010000384          772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000384 DAZGCQ010000000
VERSION     DAZGCQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.390"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>772
                     /locus_tag="ACHK07_005467"
     CDS             <1..>772
                     /locus_tag="ACHK07_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO8105621.1"
                     /translation="LNPKYCGATVRDASDYLVYRFFAAVRRAIDKAGLGWYGVRTVEP
                     HHDGTPHWHMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTP
                     TSYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQF
                     FGIPPRQVWRELRRLAGQMARNPAAPQRLAHDDIDAILAAADVGCFATYITRQGGVLI
                     PRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPSLGERY"
BASE COUNT          178 a          255 c          211 g          128 t
ORIGIN      
        1 ctgaacccga aatactgcgg ggcaacggta cgcgatgcca gtgattatct ggtttaccgc
       61 tttttcgccg ccgtccgcag ggccatcgac aaggccgggc tgggctggta cggcgtgcgc
      121 acagtggagc cccaccacga cggtacaccg cactggcaca tgctggtatt caccagccag
      181 gaaaacgaag cccgtatcac tgaaatcatg cgtaacgccg ccatcaggga ggaccgcgca
      241 gagctgggtg atgatatctc cccgcgtttt aaatgcgaaa aaatcgaccc ggcaaaaggc
      301 acaccgacaa gctatatcgc gacctatatc ggtaagaacc tggacgccag cgccttcatg
      361 gggaatgacc cgaaaaccgg caaaccttac gtcgataagg aatcaggcaa aaccatggcc
      421 gaaaccgtgg agaacgccat cggctgggca ggccttcacc gcatccacca gttccagttc
      481 ttcggtatcc cgccccgcca ggtatggcgc gaactccgcc gtcttgccgg acagatggcc
      541 cgtaacccgg cagccccgca gcgtctggcc catgacgaca ttgacgccat actggccgcc
      601 gccgacgtgg gatgttttgc cacctatatc acccgccagg gtggtgtgct cattccccgc
      661 aacacgtacc tggtccgcac cgcatacgaa actgccgaag aggccaacga ctacggcgaa
      721 ttcccgcaac gcatttacgg cgtccgcgcc ccgtcactcg gggagcgcta ca
//
LOCUS       DAZGCQ010000385          762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000385 DAZGCQ010000000
VERSION     DAZGCQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.391"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            500..>762
                     /locus_tag="ACHK07_005468"
     CDS             500..>762
                     /locus_tag="ACHK07_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105622.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLK"
BASE COUNT          192 a          163 c          215 g          192 t
ORIGIN      
        1 gctaaatgcc gcatggtatc ggctgcatta cagccagtgt gttgagcgac cttattatcc
       61 atgcgcggta ttgtcgccgt attcctgcat taacagagac cgcagcccga cagggagact
      121 cctctgcgcg agtgtgcggg gataatcaaa aacgatacac accggggttt accgcgttaa
      181 cggagcgcgg agttgtcccc tcatggtcgc tggtccggtg cgatggtgga agaaactgga
      241 ttttgttgca actgataacc attatcattt tagcgggtcc tttccggcga tccgggccgt
      301 tacggggcgg cgacctcgcg cgttttcact atttatgaaa attttccggg atccatgtcc
      361 ggtttctctg caagttaact atatgaaaaa tataaaaaca ggtcttctgt gaaccggaca
      421 tgcgcaaaaa atgggcatgt gaaccggaca tgacctgttt tgttgttatt gtgaggtgag
      481 agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt taagtctgac
      541 cgatcgccag cttcgcagat tgcagaaatt gccgggtgct ccgatatcga ataagcgagg
      601 gcaactggat ctggatgcct ggcgcgattt ttacatatct tatctgagga gaagtaaaaa
      661 cgatgtgcct gatggcgata gcgaagacga ctatgaggag aaattgctta ttgccagatg
      721 ggaactgaca gcggaacagg ctgttacaca gcagttaaaa aa
//
LOCUS       DAZGCQ010000386          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000386 DAZGCQ010000000
VERSION     DAZGCQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.392"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>750
                     /locus_tag="ACHK07_005469"
     CDS             <1..>750
                     /locus_tag="ACHK07_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8105623.1"
                     /translation="VSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFLTGHHTLF
                     MSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFST
                     LSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADA
                     VKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPAL
                     DCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLY"
BASE COUNT          157 a          168 c          194 g          231 t
ORIGIN      
        1 gtttccgtag cgcagaaaag cttcgggaaa gaaatggcga tgattatgtt ctttgcgatg
       61 gttatcaaca ttatgattgc ccgtttcaca ccgtggaaat ttatcttcct gaccggtcat
      121 cacacgttgt ttatgtcgat gatggtagcg gtaattctgg caacagcagg catgaccggc
      181 attacgctta tagccgttgg ctctttggtt gttggcgtgg caatggtctt tttcccggct
      241 attgcgcatc cgtacatgaa gaaagtgacc ggttctgatg acgttgccat cggccacttc
      301 tcaacattat cttatgtatt ggccggtttc atcggcagca agtttggtaa taaagagcac
      361 tcgactgaag acatgaatgt gccgaaaagt ctgctgttct tgcgcgacac gccggttgct
      421 atctctttta ccatgagcat aattttcctg gtgacgtgcc tgtttgcggg tgcagatgcg
      481 gtgaaagagc ttagcggtgg taaaaactgg ttcatgttct ctatcatgca atccattacc
      541 ttcgcagctg gcgtgtacat cattctgcag ggtgtgcgca tggtgattgc ggagattgtc
      601 ccggcattca aaggtatttc agacaagctg gttccgaatg ccagacctgc tctcgattgc
      661 ccggtcgtct tcccttacgc acctaacgct gtactggttg gtttcctgag tagctttgct
      721 gcaggtttaa tcggtatgtt cacgttgtat
//
LOCUS       DAZGCQ010000387          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000387 DAZGCQ010000000
VERSION     DAZGCQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.393"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..278)
                     /locus_tag="ACHK07_005470"
     CDS             complement(<1..278)
                     /locus_tag="ACHK07_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8105624.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERL"
     gene            complement(336..683)
                     /locus_tag="ACHK07_005471"
     CDS             complement(336..683)
                     /locus_tag="ACHK07_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO8105625.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
BASE COUNT          162 a          211 c          182 g          188 t
ORIGIN      
        1 aggcgctcca ccgcctgctg gtaattaaat tcgtgtttcg gcttgacgta gcccggacgc
       61 aacacgcggg tttcaccacc acgatgacgc agcacttttc cttcaacaac cggggagaca
      121 taggctgcca ccggcgtttt tccggtaatt ttgtccagca tcacctcttc ggtgtggaaa
      181 ttcaccgtac ggcggaaaaa cagttccaga aacagcgcac gaaatttcac tttttgttcg
      241 gtataaccga gtaactggcg ggtcgtaaac aatcccataa atcagttcct ttcattcaga
      301 aatcagtcag gccaacgcgg tggcctgata acgtgttacg gcagcgccgc gtgactcagg
      361 gctgtgccga caaaggcgtt ggcctttttg tgttcatcca cactttcagg ccagcggatt
      421 gcctccgtcg caaaggtccc cgacttgtaa tacgtcagca ccgtctctgt gccttcaagc
      481 ggcagtacca gtatgccagc cgcactaccg gctttctgtc cgtcccagac caccagtttc
      541 ccgctggctt catccagcat caggggcgtc agtgccggtg ttgcctgaga aatcccgctg
      601 ctgcctgtgg cggtatgagc cggatcatta ccggcaaaaa tacgtacttc tgcacgttgt
      661 tcagtgatgg ttttcgttac catattgtaa aaacctcata ttgatggtca gcactgactt
      721 catggcatgg ccatgagcat ttt
//
LOCUS       DAZGCQ010000388          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000388 DAZGCQ010000000
VERSION     DAZGCQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.394"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..270
                     /locus_tag="ACHK07_005472"
                     /pseudo
     CDS             130..270
                     /locus_tag="ACHK07_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            247..626
                     /locus_tag="ACHK07_005473"
                     /pseudo
     CDS             247..626
                     /locus_tag="ACHK07_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          213 a          170 c          165 g          192 t
ORIGIN      
        1 aaattaccac tatcaaaaat cgctcatatc catagcccgg gatatgaata aaaaaatcaa
       61 caaagattaa attattcaaa cgttactgtt gcgtctgtgg tcgtaacaac atccgacgtg
      121 gtgatgtgtc aaatgagaca ctctgatgat cagagggttt atcgtgtata cggatcgtgg
      181 atggcagaaa acgttcagga tcaggtatcc attttaaacc agataatgag taagtttacc
      241 ccatccatgc cccacgcagt gagatcggat gtcattaaaa aagtcaggtc agcaggtcag
      301 tatgggaagg cgaacaactc gcaggaatgg gtgcgctgaa gttgctggat gataaacatg
      361 gcgaactgaa atcaatgcgt accgcgccaa attatttacg tcatggtgtc gccggtcgga
      421 ttttacgcca cattttgcag gtcgcccatg acagatgcct tcatcgcctg agtttagaaa
      481 cgggtacaca ggctggattt acggcctgcc atcaacttta tttgaagcat ggtttcgctg
      541 attgcgaacc gtttgctgat tatcgacttg atccacacag tcgattttta tcattgacgc
      601 tatgcgaaaa taatgaattg ccatgagcca gacgcagcac attcttgcat tcgacgtgct
      661 gcgcctttat ttatcaccag caggaaacgc cttgtccata gacgcccctt ccacatgcgc
      721 cacaagaaac ctctattcca
//
LOCUS       DAZGCQ010000389          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000389 DAZGCQ010000000
VERSION     DAZGCQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.395"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..651
                     /locus_tag="ACHK07_005474"
     CDS             118..651
                     /locus_tag="ACHK07_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8105626.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            648..>732
                     /locus_tag="ACHK07_005475"
     CDS             648..>732
                     /locus_tag="ACHK07_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8105627.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRD"
BASE COUNT          182 a          162 c          208 g          180 t
ORIGIN      
        1 gattttgata atccgccctt taaaaaagtg ccggcttact ggcttcatcc cggactgact
       61 tatctgaaag tgcgtattta gtgggcctgg gacagcggct gaatatttaa tatatccatg
      121 aacacaaaaa taaaatacgg cctgtcggct gccgttctgg cgttgattgc cgcaggggct
      181 tctgcgcctg aaatcctcga ccagtttctg gatgaaaagg aaggcaacca caccacggca
      241 taccgtgatg gtgcgggtat ctggaccatc tgccgtggag ccacccgggt ggatggtaat
      301 cctgtgattc ctggcatgaa gctgtcgaag gaaaaatgcg accaggttaa cgccattgaa
      361 cgggataagg cgctggcatg ggtggagaaa aacatcagag tgccactgac tgaaccccag
      421 aaagcgggga tcgcgtcatt ctgtccttac aacattggcc cgggtaagtg tttcccgtcg
      481 acgttttata aacgaattaa tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg
      541 tggattaagg acggtgggcg tgattgccgc acacgttcaa ataactgcta cggacaggtt
      601 attcgtcgtg accaggaaag cgcattagct tgttggggga tagatcagtg agaagagtaa
      661 ccgcgattat ttacgctctg gttatctgca tcatcgtctg tctgttatgg gctgttaatc
      721 attaccgcga ta
//
LOCUS       DAZGCQ010000390          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000390 DAZGCQ010000000
VERSION     DAZGCQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.396"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..719
                     /locus_tag="ACHK07_005476"
                     /pseudo
     CDS             1..719
                     /locus_tag="ACHK07_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          205 a          174 c          192 g          148 t
ORIGIN      
        1 gctgaactgg aatctgaaat tctgcaactg cgtaaggcgt taaatgaagc tcgccttgag
       61 cgagatatat taaaaaaagc aacagcgtat tttgcacagg agtcgctgaa aaatacgcgt
      121 taatcgaaca atggcgacaa caatttccca ttgaagcgat gtgtcaggta tttggtgtat
      181 ccaggagcgg ttattacaac tgggtacagc atgaaccctc agacagaaaa caaagtgatg
      241 agcggctaaa actggagatt aaggtggcac atatccgcac tcgcgaaaca tatggaaccc
      301 ggcggctcca gacggagctg gcagagaatg gcatcatcgt tggtcgtgac cgactggcac
      361 gtcttcgtaa ggagctaagg ctacgctgta agcagaaacg caagttcaga gcgactacga
      421 actcgaacca caatctgcca gttgcgccaa atctgctgaa ccagacgttc gctcctacag
      481 caccaaatca ggtctgggtg gcggacctga cgtatgttgc cacacaggag ggatggttgt
      541 acctcgctgg catcaaagat gtttatacgt gcgaaattgt cggctacgcc atgggagagc
      601 gcatgacaaa agagctgaca ggtaaagccc tgtttatggc gctcaggagc cagcgcccac
      661 ctgccgggct aatccaccac tccgatcgtg gttcacagta ctgcgcatac gattaccgg
//
LOCUS       DAZGCQ010000391          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000391 DAZGCQ010000000
VERSION     DAZGCQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.397"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..499)
                     /locus_tag="ACHK07_005477"
     CDS             complement(<1..499)
                     /locus_tag="ACHK07_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO8105628.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNITPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLICLLSGCHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLAS"
     gene            complement(515..>719)
                     /locus_tag="ACHK07_005478"
     CDS             complement(515..>719)
                     /locus_tag="ACHK07_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO8105629.1"
                     /translation="YENISVVLSKRRDIIEQAPTYEISEPVFAYEKAMPVSILLALIS
                     VATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          243 a          124 c          128 g          224 t
ORIGIN      
        1 ttgacgcaag tggtaacggc aattgtatgt ggtcaacaaa atcaggaaat aataaattaa
       61 atcgttgctg cattgccaat ggttgttgcc ttatataggg taacaacgtt gaaaaaccac
      121 atgtgatgat attgtgatag ttagcgatgc cgggtttgta tcgtgccttt tcatttattt
      181 ccaccggtat agctgataaa taatccctta gcacagcagg aaccttataa aaaaatccgc
      241 gttctgcaaa cctttctcga taaaagtgac aaccactgag taaacagatc aatggaaata
      301 aattccaatt gtttatgtag taaataagtg aactattcag attaaaatgt tcaactgaaa
      361 gattgtactg cagaagaatc atttcattgg taatagatct aagcaccgga ttgttaatag
      421 gcgtaatatt caagcttact cgttgaggat gaatataact aattggatca tatataatct
      481 ttcttagggc aagattcata acgctctcat atgtctactt aattttcaac ctgactaaat
      541 tagttagaat ggccctatac ttccataaca gccagcaagt cgctacggat attaatgcaa
      601 gtaagataga aaccggcata gccttttcat aagcaaaaac aggttcgcta atttcatatg
      661 ttggtgcttg ctcaataatg tctcttcgtt ttgacaatac aacagaaata ttttcatat
//
LOCUS       DAZGCQ010000392          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000392 DAZGCQ010000000
VERSION     DAZGCQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.398"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>707
                     /gene="sctW"
                     /locus_tag="ACHK07_005479"
     CDS             <1..>707
                     /gene="sctW"
                     /locus_tag="ACHK07_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFO8105630.1"
                     /translation="DIITEDNEDIEAEMPKMGDDPAGKEARFLQATDEMSAALTQFMK
                     KKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAATTHDYEVYSFAKKLFPDESDL
                     VVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFINSGINSALKAKLFGQALSLNP
                     KLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVTKFIKETLFSDINALDASCSSL
                     EFGMFLNKLSQLLSLQSA"
BASE COUNT          248 a          116 c          152 g          191 t
ORIGIN      
        1 gacattatca cagaagataa tgaagatatt gaagctgaaa tgcctaaaat gggggatgat
       61 cccgcaggta aagaagctcg gttcttacaa gctactgacg agatgtcagc agcgttaacg
      121 cagtttatga agaaaaaaat ctacgaagaa caactggcca attttctgga cggagaagaa
      181 tatgtcctgg aagaccagcc aatagaaaaa accgacaaag ttatggaggc gcttaaggct
      241 gcaactaccc atgactatga agtgtatagc tttgctaaaa aattattccc agatgaaagc
      301 gatctggtag ttgtgttacg tgcaatttta cgtaaaaaac aaatatcaga aaatgtccgg
      361 ctaaacgctg aagcattact tagaaaagtt aatcaagaga ctactaaaaa attcattaat
      421 agcggcatta actcggcatt aaaagcaaag ttatttggcc aggcgctgtc attaaatcca
      481 aaattactaa gggcgagtta tcggcaattt ttaatggcag aagacgatgc tgtagatact
      541 tatgtcgaat ggattggtag ttatggctat caaaatagaa tgttggtgac aaaatttatc
      601 aaagagacgc ttttctcaga tataaatgca cttgatgcga gttgttcatc attggaattt
      661 ggtatgtttc tcaataagtt gtcacaacta ttgtctcttc agtcggc
//
LOCUS       DAZGCQ010000393          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000393 DAZGCQ010000000
VERSION     DAZGCQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.399"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..604
                     /locus_tag="ACHK07_005480"
     CDS             <1..604
                     /locus_tag="ACHK07_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFO8105631.1"
                     /translation="QHDHIWYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRP
                     LINESIWNGQPDGLACSISHFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLLLLAEEN
                     NNSWIKVDSKGYWRNDSNVFPDRVYVGWMLYMPHILLPELIPEAAKIVPVFEGEKQKG
                     TIVVSTEDVFDGNNKEHIGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          238 a          125 c          137 g          202 t
ORIGIN      
        1 gcagcacgat cacatatggt acttaactgg ctactcaaga aaggacgctt caaagcatgt
       61 cgtgtttagt gataatgaac cgaaccaaaa tacaatagat acctttgagc ggcattataa
      121 aaagaatcgt cctttaatta atgaatctat atggaatgga caacccgatg ggctggcttg
      181 ctcaatatca cattttatga aatttattga taaccctaaa aaactaaatc ttatcataga
      241 tattgatcag agagttgcca atacttcgga tatgattgat tccttgcttc tgttggctga
      301 agaaaataat aattcatgga taaaagtcga ctcaaaaggt tattggcgta atgacagtaa
      361 tgtttttcct gacagggtat atgtgggatg gatgttatat atgccccaca tactcttacc
      421 tgaattaatc cctgaagccg caaaaatagt accggttttc gagggcgaga agcaaaaggg
      481 aactattgtt gtttcaaccg aagacgtatt tgacggaaat aataaggaac acatcggaaa
      541 agctaatgat attgaaataa gacttctgga tcttggactg ctacctttgc taacagagct
      601 ttaatccaac cttatcactc atgggtagat gacgggacag ttacacccgt taatggcctt
      661 atttttaaca catcagataa aattgaatgc tcaccattaa ga
//
LOCUS       DAZGCQ010000394          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000394 DAZGCQ010000000
VERSION     DAZGCQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.400"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          196 a          130 c          173 g          202 t
ORIGIN      
        1 acaaagaaca tttgacagct catcaaaata gaataaatgt tgctgaggtt gctgattctg
       61 acaagagcga atttagtaga attaaaacct cttcggattt tgatggggca ttatggactg
      121 ggataagaaa gcttatttca ttctttaaaa aatagatcta tagcgtatcg ttccggcgaa
      181 ctcatcctga tgcgttttcg ggggtccatc ggtgaaacat acgaatgatc gccctctgac
      241 gatggatgat ttattttgtt gcacagaagc gatcgctggg caggtaccgg aggttggctc
      301 tgaagctcta attgcgtcag gttgcgaagt gttgacacgg gtattggtga gaactgcccc
      361 gggaacgact ctgggctgat tctggttctg attcgctgtc agcccgtgtg gttactggcc
      421 tggctgattt cctgtcgtca ggcgtttaac tcccgactgc aggtatcact taccaagaga
      481 aggtaaatcc ttttagacgt acagtgaaag ttgtctggtc ataatctgaa gcatctgaca
      541 aagttgtgtc caccaaataa taagtagtgg gaaccaaagt gtcatatata cagaaaaata
      601 taactcaccg atttccttca cgttaatacg cagggggagg tatataacag tccgttcagg
      661 tattggataa atatttagag tggaatgatt acagttatac t
//
LOCUS       DAZGCQ010000395          697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000395 DAZGCQ010000000
VERSION     DAZGCQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.401"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..>697)
                     /locus_tag="ACHK07_005481"
     CDS             complement(107..>697)
                     /locus_tag="ACHK07_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO8105632.1"
                     /translation="PVFLTNRQELISRGLLVPALFLAEFLILVPLYFIFFRKREGLGR
                     GSFRLDVFFTLFSLIMIIQLFVPYLLAVRKTEEWMISQMSFSGAILWINIFSSVLLVP
                     VYEEIVFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISLVLI
                     SAKIKSNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          270 a          113 c          116 g          198 t
ORIGIN      
        1 aaacaaatat gagattatct aaatatatta gtaaacgatc ccttataagg aattatttgc
       61 ttctttttcc aacttcaggt gttatagata tctcataata acaatttcag actgttatgt
      121 gatattgtaa cgtatattgt attcctgtta tgactgcatt catcgccatg tgaagtaata
      181 tgggcattaa caggccattc gactttattt ttgcactgat aagaactaac gatatcagaa
      241 agagcatcaa aaatgtacga aagtcagtat attgtagatg taatgcagaa aatatgactg
      301 atgttacaat agcagagatg taaatattgt cattaaacca gaatttaaaa gagttaaaaa
      361 gacaccctcg aaatacaatc tcttcatata caggaacaag aagtacggaa gaaaatatat
      421 taatccataa aatggccccg gaaaaagaca tttgcgagat catccattct tccgttttcc
      481 tcactgccag cagataaggg acaaataact gaataatcat tattaaagag aaaagagtaa
      541 aaaaaacatc cagtcgaaaa gagcctcttc ctaatccttc tctttttcgg aagaaaataa
      601 aatacagtgg aacaagaatt agaaattcag caagaaataa tgctggaaca agaagtcccc
      661 tggatataag ttcctgcctg tttgtaagga atacagg
//
LOCUS       DAZGCQ010000396          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000396 DAZGCQ010000000
VERSION     DAZGCQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.402"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..686
                     /locus_tag="ACHK07_005482"
     CDS             105..686
                     /locus_tag="ACHK07_005482"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8105633.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYRDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALSDAVYQAKVVSEIWQRFTTPFLNI"
BASE COUNT          191 a          188 c          190 g          124 t
ORIGIN      
        1 gagacagcga aagagacaca cacctgtcca caggaaacct gcgcagccat tgaacgactg
       61 attgcaatac tcgccaaccc agacgaactg aaggggtgcg cataatgaac catctcatga
      121 ttgacaccga aacactgggt aacggaccgg acgcagccat attcgcgatc ggggcggtgt
      181 ttttcgaccc gttcaccgga aagctgggca agcagtttga gaaattcatc gacccggtag
      241 attcagaacg caacggcgga accgtcagtg caacaaccgc tgtatggtgg gccggacaat
      301 ccgtggaggc cagggcctgc ctgcgcaacg cagagggaac agagcttgca gcagtaaccg
      361 aatttctggc gttcatttca cgcaatatgc atgacgaaag cccgggcaat tcactgacca
      421 tatggtgcaa gggcgcatcc ttcgatttcc caatcctgag atccgccata aagcgaacaa
      481 ccggagaaaa aagtattccg tggtgttacc gggacgagcg ctgtatgcgt ccgcttatcg
      541 ccatggctga atcaaccggc tggagaatgc caggccgatc aggaaaagag gccattgctc
      601 acaccgcgct aagcgacgcc gtttaccagg caaaggtcgt gtcagaaatc tggcagcgct
      661 tcacaacgcc attcctgaac atatgaggtg aag
//
LOCUS       DAZGCQ010000397          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000397 DAZGCQ010000000
VERSION     DAZGCQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.403"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHK07_005483"
     CDS             complement(<1..143)
                     /locus_tag="ACHK07_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HFO8105634.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSL"
     gene            complement(160..>692)
                     /locus_tag="ACHK07_005484"
     CDS             complement(160..>692)
                     /locus_tag="ACHK07_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8105635.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPITDIDPLGLATCLYSITLSMLS
                     CVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGVWSWNVNGPG
                     ATNRKPNGIRLVPSANTETYNRDGFLTHSCLNAFGPSLGPRFCFEGCITGSSNDMQKL
                     NELIFSEPDSTLTVTD"
BASE COUNT          212 a          143 c          115 g          222 t
ORIGIN      
        1 agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca taatattgct
       61 ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact cgcagaaata
      121 gagaaaaaaa tacaattgtt aataaccttg ccatattttt tagtccgtaa ctgttaatgt
      181 gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag atgaaccagt
      241 tatacaaccc tcgaaacaga atcgaggtcc taaagacgga ccgaaagcgt ttaaacaact
      301 atgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta ctaaacgtat
      361 accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc ataccccttg
      421 aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac attgcatatt
      481 attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt tatcatcatt
      541 ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac aggtcgctaa
      601 tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat tccatccccc
      661 tttcagccca atcggatcct gagtgatata tc
//
LOCUS       DAZGCQ010000398          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000398 DAZGCQ010000000
VERSION     DAZGCQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.404"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..686
                     /locus_tag="ACHK07_005485"
     CDS             105..686
                     /locus_tag="ACHK07_005485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO8105636.1"
                     /translation="MNHLMIDTETLGNGPDAVIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALGDAIYQAKVVSEIWQRFTTPFLNI"
BASE COUNT          190 a          185 c          188 g          129 t
ORIGIN      
        1 gagacagcga aagaaaaaca cacctccccg ctggaaacct gttcagtcat tgaacgactg
       61 attgcaatgc tcaccagcca ggacgaaccg aaggagcccg tacaatgaac catctcatga
      121 ttgacaccga aacactgggt aacggaccgg acgcagtcat attcgcgatc ggcgcggtgt
      181 ttttcgaccc gttcaccgga aaactgggca agcagtttga gaaattcatc gacccggtgg
      241 attcagaacg caacggcgga accgtcagtg caacaaccgc tgtatggtgg gccggacaat
      301 ccgtggaggc cagggcctgc ctgcgcaacg cagagggaac agagctggca gcagtgaccg
      361 aatttctggc gttcatttca cgcaatatgc atgacgaaag cccgggcaat tcactgacca
      421 tatggtgcaa gggcgcatcc ttcgatttcc caatcctgag atccgccata aagcgaacaa
      481 ccggagaaaa aagtattccg tggtgttact gggacgagcg ctgtatgcgt ccgcttatcg
      541 ccatggctga atcaaccggc tggagaatgc caggccgatc aggaaaagag gctattgctc
      601 acactgcact gggcgatgcc atttaccagg caaaggtcgt gtcagaaatc tggcagcgct
      661 tcacaacgcc attcctgaac atatgaggtg aa
//
LOCUS       DAZGCQ010000399          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000399 DAZGCQ010000000
VERSION     DAZGCQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.405"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>689
                     /locus_tag="ACHK07_005486"
     CDS             <1..>689
                     /locus_tag="ACHK07_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105637.1"
                     /translation="PGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQPINSTF
                     SGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTPLTR
                     QAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSVQTMFSD
                     TNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNMTGQNLS
                     GFVLTLSRFYF"
BASE COUNT          253 a          144 c          121 g          171 t
ORIGIN      
        1 cccaggtatg ataaatacat atcacggcga tatttataac cgcacaacag ataccgacaa
       61 tgttaaaaca ccagacacac cgatgtggcc ttgtgataat cgggaagaac aacaacccat
      121 taattccaca ttctctggag aaggatacaa ccctgaacaa tatgatttag cgcggcaaca
      181 cctcccacaa atttatgcgt gccgcacgaa caccaaatat acagatgcag actactcaaa
      241 agttgtggct caacttgtaa gtcttattac taacattgaa tcgataagtt cgacaccact
      301 tacgcggcag gcacaggaaa tacttaatca aattaataac attagatacg aaaagaacaa
      361 aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg caatcataac
      421 taaaattagc gtagaagagg gaataccagt aagattcagt gtccaaacca tgttttcaga
      481 tactaatttt attgctgaac aacgagctga cttaccgact aatataaaag atattcaaag
      541 cctgtatcaa aaaatgacga agttatatat tgagcacagt gaaaataaaa acaggatgaa
      601 agtctttgcc ggaaccaact ttattgattt caatatgaca ggacagaacc tgtccgggtt
      661 cgttctaacc ttgtcaaggt tttatttcg
//
LOCUS       DAZGCQ010000400          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000400 DAZGCQ010000000
VERSION     DAZGCQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.406"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            513..>683
                     /locus_tag="ACHK07_005487"
     CDS             513..>683
                     /locus_tag="ACHK07_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO8105638.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPV"
BASE COUNT          165 a          169 c          180 g          169 t
ORIGIN      
        1 attgctggtt tcgtggtgtg cagcaatgta gttacagtgc aatcaatgtc acaattcagt
       61 cagttgacag ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
      121 cctgagcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
      181 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
      241 ttaggcatat atccaggctc ctcatcgcag gagccttttt acatgcaaaa aaagcccgcg
      301 ccgggagacg cgggcagcaa ggaataaaca acaaaacgtg aagtaatcaa tttttcagct
      361 ggcgaataat acccgacagt aatcactctg cgcaactgcg cggccttttt cgtattgcgg
      421 gctgtagtct tccttctgtc attgtcctgt aacttccgga cttcagcccg ccccttatct
      481 gactcacaac attatcccgg ccgggaggat tcatggcatt taaacactat gacgtggtca
      541 gggcggcgtc gccgtcagac cttgcggagc gactgactca aaaactgaag gaggggtggc
      601 agccatttgg cagcccggtg gccatcacgc cttataccct gatgcaggcc attgcggcgg
      661 aaggtgatgt caccacacct gtg
//
LOCUS       DAZGCQ010000401          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000401 DAZGCQ010000000
VERSION     DAZGCQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.407"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>675
                     /gene="espX1"
                     /locus_tag="ACHK07_005488"
     CDS             <1..>675
                     /gene="espX1"
                     /locus_tag="ACHK07_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO8105639.1"
                     /translation="YCSTKNTITNKFDVVFSYCGDNVKEFILLLPYNKSLEMHELNEQ
                     KIQYLTALNININKLLLSNITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEIN
                     NLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGFRYTGVFKTLASIDDK
                     PSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMK
                     KISHQD"
BASE COUNT          255 a          120 c           98 g          202 t
ORIGIN      
        1 ttactgctca actaaaaaca caatcaccaa taaatttgat gttgtctttt cttactgcgg
       61 agataatgta aaggaattta tacttttatt accctataat aaatctctgg agatgcatga
      121 acttaacgag caaaagatcc agtatttgac tgcccttaat attaatataa ataaactatt
      181 gctatcaaat ataacaatag aaaagagcaa cctttcatat gggtattatt ttggttgtgt
      241 attatccaat atatcatgtt ttgaaagcga cttatcaaac accatctttt caaatggaga
      301 aataaacaac ctcttcatta aaaaaagcaa catatttgga acatcgttca ctaataccat
      361 gataaaaaat ttgcgctgtg aagatattat gcctggccga tggaccactc aacttgtcaa
      421 taagcactta ggtttcagat acacaggagt attcaaaacc ctggcaagta ttgatgacaa
      481 accgtcccgt ttcgaaattt tgataccatt ggtacaaact ttagttcgcg acaatgtaaa
      541 gctaaataat gatgtgtata aagaactgaa aaaatttatg cacgattacg acaaaacatc
      601 cccagaaatg agaaaatacc ttcagagcat caacgagtct atgttcctta tgaaaaaaat
      661 atctcatcaa gatta
//
LOCUS       DAZGCQ010000402          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000402 DAZGCQ010000000
VERSION     DAZGCQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.408"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>662)
                     /locus_tag="ACHK07_005489"
     CDS             complement(<1..>662)
                     /locus_tag="ACHK07_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8105640.1"
                     /translation="VTAGNGATVAPTVITEPDGTVEISVTSQTAGISTVTASINSSSL
                     SRDVMFIADIRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNTLAGQTVSVMADN
                     GATVASTVTTGQDGTVEISVTSQTAGTSVVTTSINSSNASRNVTFIADVRTAQIADLV
                     VIKDGSEADGATANTLRARVTDAFGNALAGQTVSVSADNSAMVTPTVTTQPDGTVEIS
                     VT"
BASE COUNT           99 a          239 c          176 g          148 t
ORIGIN      
        1 ctggtgacac tgatctccac cgtgccatcc ggctgcgtgg tcaccgtcgg agtgaccatt
       61 gcgctgttgt ctgccgatac gctgaccgtc tgcccggcaa gcgcattgcc gaacgcatcg
      121 gtcaccctcg cccgcagcgt attcgccgtc gcaccgtccg cctctgaacc atccttaatg
      181 accaccagat ccgcgatctg cgccgtcctg acatccgcaa taaacgtcac gttcctgctc
      241 gcgttgctgc tgttgatgct ggtggtgacc acactggtgc ccgccgtctg gctggtgact
      301 gagatctcca ccgtgccgtc ctgccctgtg gtgaccgtcg aagcgaccgt tgcgccgttg
      361 tccgccatca cgctgaccgt ctgcccggca agcgtattac cgaacgcatc ggtcaccctc
      421 gcccgcagcg tgttcgccgt cgcaccgtcc gcctctgaac catccttaat gaccaccaga
      481 tccgcgatct gcgccgtcct gatatcggcg ataaacatca cgtcccggct caggctgctg
      541 ctgttgatgc tggcagtgac tgtactgatc cccgccgtct ggctggtgac actgatttcc
      601 accgtgccgt ccggctctgt aatcaccgtc ggggcaaccg ttgcgccgtt gcctgctgtc
      661 ac
//
LOCUS       DAZGCQ010000403          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000403 DAZGCQ010000000
VERSION     DAZGCQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.409"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>661
                     /locus_tag="ACHK07_005490"
     CDS             <1..>661
                     /locus_tag="ACHK07_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8105641.1"
                     /translation="DGRVTEQLNPAGLSYTYCYEQNRITITDSLDRREVLHTQGEGGL
                     KRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLELRREYDESGRLIQETAHNGDITRYRYDNPHSDLPCATDD
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IA"
BASE COUNT          164 a          188 c          201 g          108 t
ORIGIN      
        1 gcgacgggcg ggtgacagag cagcttaacc cggcgggctt aagctacacc tactgttatg
       61 agcaaaaccg catcaccatc accgacagcc tggaccgccg tgaagtgctg cacacgcagg
      121 gcgaaggcgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc gtcacgcaga
      181 gtcagtttga tgcggtgggc aggcttaagg cacagacgga tgccgcaggc cggacaacag
      241 aatacagccc ggatgtggtg acgggactca tcacgcgcat caccacaccg gatggcaggg
      301 catcggcatt ttactataac caccacagcc agttaacgtc agccaccggg cctgacgggc
      361 tggaattgcg ccgggaatat gatgaatccg gccgtctgat tcaggaaact gcacacaacg
      421 gcgatatcac ccgctaccgt tatgataatc cacacagtga cttaccctgc gcaacggacg
      481 atgccaccgg cagccggaaa accatgacgt ggagccgtta cggccagttg ctgagcttca
      541 ccgactgttc cggttatgta acccgttatg accatgaccg tttcgggcag atgacggcgg
      601 tgcaccgcga ggaagggctg agtcagtacc gcgcatacga cagccgtgga cagttaattg
      661 c
//
LOCUS       DAZGCQ010000404          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000404 DAZGCQ010000000
VERSION     DAZGCQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.410"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..539)
                     /gene="tnpA"
                     /locus_tag="ACHK07_005491"
     CDS             complement(81..539)
                     /gene="tnpA"
                     /locus_tag="ACHK07_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO8105642.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          152 a          181 c          121 g          200 t
ORIGIN      
        1 ttttcaaatg cgcctataag gctctcttac cagccgcgcc ctaacaggcg cacacgatct
       61 gacatttgca tccaacttcg ttacttacgg cccgtaaacg ggctgcccgg atagggaatc
      121 gataactgct ctcccatttt atcctcttca agctggtgct ttatgtaatc ctgtatcttc
      181 gccgtgttct tacccaccgt atcgacgtag taccctctgc accagaactc tctgttcctg
      241 tatttgaatt tcaaatcacc aaactgctcg taaagcatca gactgctttt ccctttcaga
      301 tatcccataa agccggatac gctcattttg ggcgggatct ccacaagcat atggatatga
      361 tctgcacagc attcagcttc aagaatccgt acgcttttcc actcgcacag ctttctcaaa
      421 atactgccta ttgctctacg tttctctctg tagaacacct gtcttcggta ttttggcgca
      481 aaaactatgt gatatttaca gttccatcgg gtgtgcgcta agctcttttc gttccccatt
      541 gggaccccct tttgatttct tgtttgacac ttgcagttgc cagaccgcaa ggtgttttaa
      601 caaatcaaaa ggggttttaa taactggctc aaagctgaaa gctttccgga accc
//
LOCUS       DAZGCQ010000405          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000405 DAZGCQ010000000
VERSION     DAZGCQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.411"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>641
                     /locus_tag="ACHK07_005492"
     CDS             <1..>641
                     /locus_tag="ACHK07_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8105643.1"
                     /translation="QLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGGLKRVVKK
                     EHADGSVTQSQFDAVGRLKAQTDTAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHH
                     NQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRK
                     TMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIA"
BASE COUNT          159 a          186 c          191 g          105 t
ORIGIN      
        1 cagctaaacc cggcaggctt aagctacacg tatcagtatg agaaagaccg catcaccatc
       61 accgacagcc tggaccgccg tgaagtgctg cacacgcagg gcgaaggcgg gctgaagcgg
      121 gtggtgaaaa aggaacacgc ggacggcagc gtcacgcaga gtcagtttga cgccgtgggc
      181 aggcttaagg cacagacaga tacggcaggc cggacaacag aatacagccc ggatgtggtg
      241 acgggcctca tcacgcgcat caccacgccg gatggcaggg catcggcgtt ttactataac
      301 caccacaacc agttaacgtc agccaccggg cctgacgggc tggaattgcg ccgggaatat
      361 gatgaatccg gccgtctgat tcaggaaacc gcccctgacg gcgatatcac ccgctaccgt
      421 tatgataatc cacacagtga cttaccctgc gcaacggacg atgccaccgg cagccggaaa
      481 accatgacgt ggagccgtta cggccagttg ctgagcttca ccgactgttc cggttatgta
      541 acccgttatg accatgaccg tttcggtcag gtgacggcgg tgcaccgcga ggaagggctg
      601 agtcagtacc gcgcatacga cagccgtgga cagttaattg c
//
LOCUS       DAZGCQ010000406          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000406 DAZGCQ010000000
VERSION     DAZGCQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.412"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>641
                     /locus_tag="ACHK07_005493"
     CDS             <1..>641
                     /locus_tag="ACHK07_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105644.1"
                     /translation="ELRICASCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYE
                     RPYNSFISDATANGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNAR
                     ILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPR
                     DQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESV"
BASE COUNT          177 a          158 c          171 g          135 t
ORIGIN      
        1 gagcttcgta tctgtgcctc ctgcgggctg acagaagtac atcatgcccc ggaaaatcat
       61 aagccggatc cggaatggta ctgctcctct ctttgtcgcg aaacagaaac actgtgtcag
      121 gaaatttatg aacgccctta caacagcttt atttccgatg caacggcgaa tggtctgatt
      181 ctcatgaaac tgccggaaac ctggagtaca aatgagaaaa tgtttgcttc cggagggcag
      241 ggacatgggt ttgccgctga acggggaaac catattgtcg acagagtccg tctgaaaaac
      301 gcacggatcc tcggtgataa taatgccaga aatggagcag acagactggt cagcggaaca
      361 gaaatccaga cgaaatattg ttcaactgca gcccgtagcg tcggtgcggc attcgacgga
      421 caaaacggac agtatcgtta catgggaaat aatggcccca tgcaactgga agtcccccgt
      481 gatcagtatg ccggcgctgt tgaaaccatg aggaataaga tccgcgaagg taaagtaccc
      541 ggtgtaaccg atcccgcaga agcgtcccgg ctgattcgtc ggggacatct gacttatact
      601 caggcccgta atatcacccg gttcgggacc atcgaatcgg t
//
LOCUS       DAZGCQ010000407          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000407 DAZGCQ010000000
VERSION     DAZGCQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.413"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>626
                     /locus_tag="ACHK07_005494"
     CDS             <1..>626
                     /locus_tag="ACHK07_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8105645.1"
                     /translation="NEENPHHLHQPYRLPGQQYDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSH
                     SEMIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQDAG"
BASE COUNT          200 a           99 c          162 g          165 t
ORIGIN      
        1 aatgaggaga acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat
       61 gaggagtcgg ggctgtatta taaccggaac cggtactaca atccgttgct gggacgatat
      121 attactcagg acccgattgg gctggcgggg ggatggagtc tgtatgcgta tccgctgaat
      181 ccggtgaatg gtattgatcc attagggtta agtcctgcag atgtagcgtt aatgcgaaaa
      241 aaagaacaac taaaccatca aagagcatgg gatatattat ctgatactta tgatgatatg
      301 aagagattaa atttaggtgg gactgatcaa tttttccatt gtatggcatt ttgtcgagtg
      361 tctaaattaa atgacgctgg tgttagccga tcagcgaaag ggctgggtta tgaaaaagag
      421 attagagatt acgggttaaa tatgttcggt atgtacggca gaaaagtaaa gttatcccat
      481 tctgagatga ttgaagataa taaaaaagac ttggctgtaa atgaacatgg gttgacatgt
      541 ccattaacac aagattgctc aaatagatgt attgattata ttaatccaga gcataaaaaa
      601 acgataaagg ctttacaaga tgctgg
//
LOCUS       DAZGCQ010000408          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000408 DAZGCQ010000000
VERSION     DAZGCQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.414"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>615)
                     /locus_tag="ACHK07_005495"
     CDS             complement(<1..>615)
                     /locus_tag="ACHK07_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105646.1"
                     /translation="INNDGNKILILNYLITNIFTSTNSNPDGLLFDAILDIAHNSVHR
                     GYLNQHSLIRHRCVQHALINIISKQTDIDLKIKWIKDIVLVLKLQHDDLSKCISPLIF
                     AIESIKTTSINDTTIKTSSQILKLHNYMEMCYTLFDSYPNLITECNDLFTDCISIVTQ
                     YDLRGYFSRLFEMIEITDNETKKNTTAFLISRIESRHLYQPKIA"
BASE COUNT          196 a           88 c          103 g          228 t
ORIGIN      
        1 cggcaatttt aggttgatac aaatgccggg attcaattct gctaattaaa aaagcagttg
       61 tattcttttt agtttcattg tcagtaatct ctatcatctc aaaaagtctg ctaaaatatc
      121 ctctcagatc gtactgagta acaatagaaa tacaatccgt gaataagtca ttacattcag
      181 tgattaaatt tggatagcta tcaaaaagtg tataacacat ctccatataa ttatgcagtt
      241 ttaatatttg cgaagaggtt ttaattgtcg tgtcgttaat gctggttgtt tttatcgatt
      301 caatagcgaa aataagtggg ctgatacatt tcgataaatc atcatgttgt aatttcaaca
      361 ctaaaactat atcttttatc cattttattt ttaagtcaat atctgtttgt tttgagatga
      421 tgttaatcag tgcgtgctgg acgcagcgat gcctgattaa actatgttga tttaaatatc
      481 ctctgtgaac ggagttatgt gcaatatcca gtatagcatc aaagagcaaa ccatcaggat
      541 ttgaatttgt ggatgtaaag atgttcgtta taagataatt taatatcaat attttatttc
      601 cgtcgttatt tatat
//
LOCUS       DAZGCQ010000409          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000409 DAZGCQ010000000
VERSION     DAZGCQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.415"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(294..>614)
                     /locus_tag="ACHK07_005496"
     CDS             complement(294..>614)
                     /locus_tag="ACHK07_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8105647.1"
                     /translation="RSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELT
                     TFSELLLKERMQYAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPV
                     MQPD"
BASE COUNT          191 a          157 c          133 g          133 t
ORIGIN      
        1 aaaaatgtat cctgcgtctg ctcaggagca tcaagcagga caacacgaac acctgctggc
       61 gggttcatgg ggcgctggtg cgaatcggta taaattgttg taccggcaga tgcagccacc
      121 ggtagtatca ggaaggaata cagtaacagt cggtacatga tgcgactccg tcagacaact
      181 ggtatctgac agcgtgcgca aaggcaaagg gacaatcagc aataaattca aaaccggatg
      241 ataaaaatta agcctcttcc tctgaataac gcacactctg catctgaatc aaattaatcc
      301 ggttgcatca caggaagcca ctgattcggg gtaataccat aataacgcct gaacacagaa
      361 ataaaataag aagcactggc atagccacac aactctgcca cccgggaaat acggttttct
      421 cccgcagaca aaagctgagc ggcatactgc attctctctt tcagaagaag ctcactgaag
      481 gtcgtcagct cttcctgaag ctttttcttc agtaaactct cacttatata taacagactg
      541 gccacttcac ccagcatcca tggacgggac aaatcagcac tgataatatt cttcacccgg
      601 acagtgcagc tccg
//
LOCUS       DAZGCQ010000410          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000410 DAZGCQ010000000
VERSION     DAZGCQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.416"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..122
                     /locus_tag="ACHK07_005497"
     CDS             12..122
                     /locus_tag="ACHK07_005497"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8105648.1"
                     /translation="MGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGWQW"
     gene            147..>607
                     /locus_tag="ACHK07_005498"
     CDS             147..>607
                     /locus_tag="ACHK07_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO8105649.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTL"
BASE COUNT          166 a          113 c          184 g          144 t
ORIGIN      
        1 gcgccattgc aataggtgtg tcccgaatct ccgataacgg taagatgata atgaaactga
       61 atgcaggtca gaacacccgg ggtaatttct ccgtcggtgc cggtgtgggc tggcagtggt
      121 aacagggaaa agcaaggaga aatgtaatgt tatttagtcg aatgatgatg acgggtgtgc
      181 tgtccctgat gcttgcagga tgtggaaatc tgagtcatgt cacggatgaa ggaacgacgg
      241 aagaagtggt atggccttct ccggagaagg tgatgtttaa tcatgacgga acacagaaag
      301 gttcgtggcc actgccagaa aagttacgtc agataaaggc gggtatgaat aaggaccaga
      361 tttatcagct ggcggggaga ccacactttg ctgaagggat gtctggtgta agggaatggg
      421 attacctgtt caactttacc ggaccggagg gggattatac ctgccagtac aagatactgt
      481 ttgaccggaa catggatgcc cgctctttct actggaagcc ggaaaactgt ctgccggagg
      541 cagagacagc agaatacagg gagtcagtcc ccggtgatat attatttgat tttgattcat
      601 ggactct
//
LOCUS       DAZGCQ010000411          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000411 DAZGCQ010000000
VERSION     DAZGCQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.417"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..350)
                     /locus_tag="ACHK07_005499"
     CDS             complement(<1..350)
                     /locus_tag="ACHK07_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO8105650.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSANDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGAR"
     gene            complement(446..>598)
                     /locus_tag="ACHK07_005500"
     CDS             complement(446..>598)
                     /locus_tag="ACHK07_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8105651.1"
                     /translation="GVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYL
                     CSTADR"
BASE COUNT          177 a          156 c          109 g          156 t
ORIGIN      
        1 cgtgccccct ctgttccgtt actgccataa agcagcatca tcagtacatt tacctgccgg
       61 cctgacaggg tcagcttctc caccgacatg aatgcgcggt acagatgact gatatcgttt
      121 gctgaacatc gctcagtaag atgtctgata tcataaaccg gactgtcaac cactccccag
      181 agaaaacgga gcgcttccgg agaagaaaaa taaagggtgt tcgtatcact tatattattc
      241 ttatacagaa aaccgacaat ttcagatatc cgggaagaag atattatcac cacattatct
      301 gtctgcatgc cgctgataat gaatttaccg ggattaatat actccttcat tccctccaga
      361 aaataactgt tatcacaaat aaaatgcata cccgttccac atgaaattta tcacgcctgg
      421 ccacataaca ttaattatcc cggatttacc ggtctgcggt gctgcagaga tatttaatac
      481 tgacgccgcg cagaccattt ttctccatcg cattgtaaag atgcgcacat accgtcttca
      541 tgcttatccc cagctggtct gccagaaagg taaaaccgta ctcatggaaa acaactcc
//
LOCUS       DAZGCQ010000412          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000412 DAZGCQ010000000
VERSION     DAZGCQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.418"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>593)
                     /locus_tag="ACHK07_005501"
     CDS             complement(<1..>593)
                     /locus_tag="ACHK07_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8105652.1"
                     /translation="DILLWTICAVISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVH
                     DTIARVVYCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVI
                     SAFSTMHSLVIGQIRTDEKSNEITAIPGLLNMLDIKGKIITTDAMGCQKDIAEKIQKQ
                     GGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYA"
BASE COUNT          163 a          137 c          105 g          188 t
ORIGIN      
        1 attgcgtaac tgtcatgctc tggattattt aattctttca gtggaaattt ttcctcaaag
       61 gctttattaa gccgcccctg atttcctttt acagcgaata aataatcacc gccctgtttt
      121 tgtatcttct ctgcgatatc tttctggcaa cccatagcat cagttgtgat aatttttcct
      181 ttaatatcca gcatgttaag aagtccaggg atagctgtaa tctcattgga tttctcatcc
      241 gtcctgatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
      301 gctcccttgc ggcgactctt gtcataagaa tgccggagtg tttttccatc aattgcgatg
      361 acgtctttat catctgaaga atggcagtca cgcatccagt taataaagca ctcgtgaaat
      421 tttgcgggac tgatacagta tacaactctg gcaatggtat cgtgaacagg aatgccattt
      481 tcaaaatcac catattgctt caaaaaatcg agatgtgttt ccccaaaatc ctctatatct
      541 tcccaacctt ctgcaccaga aataacggca caaatagtcc acagtagaat atc
//
LOCUS       DAZGCQ010000413          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000413 DAZGCQ010000000
VERSION     DAZGCQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.419"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>587)
                     /locus_tag="ACHK07_005502"
     CDS             complement(<1..>587)
                     /locus_tag="ACHK07_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO8105653.1"
                     /translation="LDERETFNVSWGIDENYQVGTAISIASILENNKQNKFTFHIIAD
                     YLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFSARL
                     DSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDTMQSKSAERLGNEDFNGSYFNS
                     GVMYINLREWLKQRLTEKFFELLSDESIIKKLKYP"
BASE COUNT          200 a          111 c           78 g          198 t
ORIGIN      
        1 ccggatattt taattttttg ataattgact catctgataa tagttcaaag aatttctccg
       61 ttagtctttg ttttaaccat tcccgtaaat taatatacat tacaccagag ttaaaatagc
      121 taccgttaaa atcctcatta cctaaacgct cagcgctttt actttgcata gtatctacat
      181 caattaccac agccccatat tcatctttaa actctaatgc tattaactcg ttcaatgaac
      241 ccttgcaaac gatatcagca tcaagatata ataatgaatc caatcgcgca gaaaaataat
      301 caaatgaaag caaacgataa taaatagata ctggccagat atttgattga ggtaacgcct
      361 tcaatggctc agaatcaata tgatataatt taattactgt ttgatacttc gttgctaatt
      421 gtgataataa ttcaatatac tctttgtcta agtaatcagc gattatgtga aaggtaaatt
      481 tgttttgttt attattttca agaattgaag caattgaaat cgcagtccca acctggtagt
      541 tctcatcaat accccatgag acattaaacg tttcccgttc atcaagt
//
LOCUS       DAZGCQ010000414          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000414 DAZGCQ010000000
VERSION     DAZGCQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.420"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..423)
                     /locus_tag="ACHK07_005503"
     CDS             complement(<1..423)
                     /locus_tag="ACHK07_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105654.1"
                     /translation="MKVVFNVKSDFDKIFLPLEFSGFHGRNGYCYLRVQIKRGFIVFS
                     CAQLLNYYRTSVTNAIEQVREAAVNVLFREGVLSYTQQKEFWDLLKTSQRVNKEIDSQ
                     LWDYINANSIWFEYYNHNESLFLNDHFHIASFEGNKNPE"
BASE COUNT          205 a          117 c           89 g          170 t
ORIGIN      
        1 ctcgggattt ttgttaccct cgaaagaagc tatatgaaaa tgatcattca gaaataatga
       61 ttcattatga ttatagtatt caaaccagat tgaattagca ttaatgtaat cccataattg
      121 cgaatcaatt tctttgttta cccgttgtga tgtctttagt aaatcccaga attctttttg
      181 ctgtgtgtat gacaacacac cttccctgaa gaggacatta accgcagcct ctctgacctg
      241 ctcaattgca tttgttactg acgtacgata gtagttcaat aattgagcgc atgagaaaac
      301 aataaaacca cgcttaatct gaactcttaa ataacagtac ccattccgcc catgaaaacc
      361 agaaaactcc agtggtaaaa aaatcttatc gaaatcactt tttacattaa agacaacctt
      421 cacttaacta aactcctgtt tgatatcttg agaatataat taatcctgag tttataaaat
      481 gaatagcgat gaccaagctg tcaacatggc atacataaac ttaaaaaaaa caacaaaaac
      541 tgacgttgca gatgtgataa cgccagtccg aagactggcg c
//
LOCUS       DAZGCQ010000415          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000415 DAZGCQ010000000
VERSION     DAZGCQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.421"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHK07_005504"
     CDS             <1..297
                     /locus_tag="ACHK07_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105655.1"
                     /translation="AFQSKSHTIKKIDAFSNYAQHPTQEGCSYFYDNKYFNSVDMLSP
                     IIEKLQKMEINCKIHPYIYIATNDSMSGFYAFTCSQMLNSESPNNCNSLYYKAE"
     gene            302..>581
                     /locus_tag="ACHK07_005505"
     CDS             302..>581
                     /locus_tag="ACHK07_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105656.1"
                     /translation="MTQRYFLFAGIILCAFIAAILSHIAFHHANEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTY"
BASE COUNT          204 a          107 c           91 g          179 t
ORIGIN      
        1 gcttttcaaa gcaaatccca cactataaaa aaaatagatg ctttctcaaa ttatgctcaa
       61 caccctactc aggaaggatg cagttatttc tatgataata aatactttaa ttccgtagac
      121 atgttatcac cgattattga aaaattgcag aagatggaaa ttaactgcaa aatacatcct
      181 tacatttaca ttgcaacaaa tgatagcatg agcggatttt atgctttcac ctgtagtcag
      241 atgttaaact ctgaatcacc aaataattgc aactctctct attataaagc ggagtaatca
      301 tatgactcaa agatattttc tgtttgcggg aataatttta tgtgctttta ttgcagcaat
      361 tctgagccat attgcgtttc atcatgccaa tgaaccagca gagcaaaaca tatcatgtaa
      421 tgcccatgtt attaatttca ccgatcatac aaaaatggat caatattttt cgttaaatat
      481 gatttctgaa aataacacag gaaacatgta tctcacgggc gcatacacgg tagatggaaa
      541 aaagatgggc ttcatccgcc gatatgtgag ttttacatac a
//
LOCUS       DAZGCQ010000416          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000416 DAZGCQ010000000
VERSION     DAZGCQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.422"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>573
                     /locus_tag="ACHK07_005506"
     CDS             <1..>573
                     /locus_tag="ACHK07_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010671752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8105657.1"
                     /translation="QNWRENNKTTPINKLLNKINEERNEARKNKKIKQNFGKYRPVLP
                     LFQLPADCWEWVCFEQICETDNNALKAGPFGSALKKSDCVKSGHKVYGQEQVISGQES
                     LETYRITEDKYQQLKSCATKPGDILISLVGTIGKTLILSNLAETGIINPRLVKIALHS
                     EIQRQYVKLYLDGPLAYDFFKGFSHGGTMD"
BASE COUNT          223 a           90 c          104 g          156 t
ORIGIN      
        1 cacaaaattg gagagaaaac aataaaacaa ccccaattaa caaattatta aataagataa
       61 atgaagaaag aaatgaagcc agaaaaaaca aaaagataaa acaaaatttt ggtaaatata
      121 gacccgtact tccattattt caattgcctg ccgattgttg ggagtgggta tgttttgaac
      181 agatttgtga gactgataac aatgcgttaa aagctgggcc atttggttcg gcactcaaaa
      241 aatctgactg tgttaaatct ggtcataaag tttatggtca agagcaagtt atctcaggac
      301 aggaatcact tgaaacttat agaattacag aagataaata tcaacaattg aaatcctgcg
      361 ccaccaaacc aggagatatt cttattagtt tagttggtac tatcggaaaa acattaatac
      421 tatctaatct tgcagagaca ggtataataa atccacgcct agtaaaaata gcattacata
      481 gtgaaattca acgccaatat gtaaagctgt atttagatgg ccccttagca tatgactttt
      541 ttaaaggatt cagtcatggc gggactatgg ata
//
LOCUS       DAZGCQ010000417          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000417 DAZGCQ010000000
VERSION     DAZGCQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.423"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..172
                     /locus_tag="ACHK07_005507"
     CDS             <1..172
                     /locus_tag="ACHK07_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO8105658.1"
                     /translation="IAMPEKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWMLSPFY
                     DMKHSATGPLND"
     gene            165..>562
                     /locus_tag="ACHK07_005508"
     CDS             165..>562
                     /locus_tag="ACHK07_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFO8105659.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGRE"
BASE COUNT          152 a          124 c          144 g          142 t
ORIGIN      
        1 cattgcgatg cctgaaaagc agattatcag atatgacgaa aaatacctcc ctctggaaaa
       61 tggaatgaaa gccgaaagta cactatttct ggaaaaaagg cgtatttacc agtggatgct
      121 ttctcctttc tatgacatga aacacagtgc aacaggaccg ctcaatgact aacgggaatt
      181 tcagacaaat tataaatcag cttgatatgc gctggcgacg tcgtgttccg gttattcatc
      241 agacggagac cgctgaatgt ggactggcct gcctggcaat gatatgcggt cattttggta
      301 agaatattga cctgatatct cttcgccgga agtttaatct ctcggcccgt ggagcaaacc
      361 ttgcaggaat caatggaata gcagagcagc tggggatggt cacccgggct ctttcactgg
      421 agctggatga acttggtgcc ctcaaaatgc cgtgtattct ccactgggat ttcagtcact
      481 tcgtcgtgct ggtcagcgta aagcgtaacc gttatgtact gcatgatccg gccagaggca
      541 gaagatatct cggtcgggag ga
//
LOCUS       DAZGCQ010000418          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000418 DAZGCQ010000000
VERSION     DAZGCQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.424"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>561)
                     /locus_tag="ACHK07_005509"
     CDS             complement(<1..>561)
                     /locus_tag="ACHK07_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO8105660.1"
                     /translation="GICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREM
                     SAVSSTAGGPENAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIG
                     GMVPGYSCESPVTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWG
                     NVYPRSGFVTQTDDDKAAAVVAQRVA"
BASE COUNT          124 a          167 c          140 g          130 t
ORIGIN      
        1 tcagccaccc gctgggccac caccgcagca gctttatcat catctgtctg ggtcacgaac
       61 ccggagcggg gatacacatt gccccacata ttctcttctt cctggctgcc tatttcgcgt
      121 ttaccgggga tcagggcctc cgggtacagt gactccggta gtcctgtacg ccaggcaata
      181 ctgtcaagcg tactgagaaa ataaggaaca aaaggtgtca ccggtgactc acaggaatat
      241 cccggcacca tccctccgat aagcgtggtg gccgggtgcc ctgttgcatc ggcatattta
      301 aaacggattg acgaacgacg ttccccctgt accttcatgt ctgcggggtt ccctcctcct
      361 gctgacagac cggcaatacc tttgcttatg gcattctccg gaccaccggc tgtactgctg
      421 accgccgaca tttcacgcca gggatttccg ccaggtgcgg tataggtgga aacaaccact
      481 tccgggatat aatgggaaac tttcactgat gttctgacct tgcaactgaa tttggtgcag
      541 aacagccagt agcaaatccc g
//
LOCUS       DAZGCQ010000419          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000419 DAZGCQ010000000
VERSION     DAZGCQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.425"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..176
                     /locus_tag="ACHK07_005510"
     CDS             <1..176
                     /locus_tag="ACHK07_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105661.1"
                     /translation="GAECWYDVVDNGTTDLRQGVVRIRYKYTPVPPLEDLELHQTFTD
                     EFFAGAFASLGGE"
     gene            176..>558
                     /locus_tag="ACHK07_005511"
     CDS             176..>558
                     /locus_tag="ACHK07_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8105662.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSM"
BASE COUNT          116 a          130 c          172 g          140 t
ORIGIN      
        1 tcggggcaga gtgctggtat gacgtggtgg ataacggcac aacggatctg cgtcaggggg
       61 ttgtgcgcat tcgctacaag tatacgccgg tgccaccgct tgaagatctt gagcttcacc
      121 agacgtttac cgatgaattc tttgcgggtg catttgcatc actgggaggt gagtaatggc
      181 ggtaccaaac aaacttcgcg ttttcacggt gtacatcgat ggcgaaaaca agctggggcg
      241 ggtgacgtcc ttcacgccgc ctaaactgac ccgaaaaacg gagtccttcc gtggtgccgg
      301 gatgcccggc agtgtttccg tcgactttgg tcttgatgat ggtgcactgg atctctcttt
      361 tgcggtaggc ggcgcagatg cccacctttt ctcccgttat gccgggtcac ttgatgaagt
      421 ccgtcttcgc ttttccggtg agtactattc ggatgcggat atcagatatg tggatattga
      481 ggtccgcggg cgcatcactg aaatcgatat gggggaggcg aagcagggtg aagacactga
      541 gcacagttat tcgatgaa
//
LOCUS       DAZGCQ010000420          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000420 DAZGCQ010000000
VERSION     DAZGCQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.426"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..458)
                     /locus_tag="ACHK07_005512"
     CDS             complement(<1..458)
                     /locus_tag="ACHK07_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8105663.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDG"
BASE COUNT          120 a          177 c          144 g          117 t
ORIGIN      
        1 tggccgtccg gctgtgctgc cttaccaccg cgcaccagcc acattgactc gctgcggctg
       61 tacaccgcct cacccggcag cagcggctca aagtgaatgc tccgtccgcc gttgtcgttg
      121 agtaccagtg catcatcgcg tagctgtaag cggatatcag aaggcgcttt ccagccgggg
      181 ccaaaaatcc ccaccggtgc aggcgtcttc gtccggtagc tgctgtaggt gcgggagaga
      241 atgaacggca gcgggccggg cagcgcaagg tccgtctcgc cgggcagcac cttcgccccc
      301 agcagcggat ttaccgggtt gcccgaggtc atcccgcccg gacacaccga gcacgccacg
      361 ccagttggcg cgccaattct tacacctgcc gaaccctgga caatgggacc gccatactga
      421 gtcatatctc cctgacgcgc cgctggtttt ccgctcatat aaagtgccct tttagttatt
      481 gttcatatcc ctgtgttagc aacaaaaaaa ataaaatgac acacaagacc agctaatgta
      541 tttatatatt tttcacat
//
LOCUS       DAZGCQ010000421          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000421 DAZGCQ010000000
VERSION     DAZGCQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.427"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..238
                     /locus_tag="ACHK07_005513"
     CDS             <1..238
                     /locus_tag="ACHK07_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105664.1"
                     /translation="MTERPKWVREPSWRFIEDKPMIFISQVEFENNKTMAEHLSSGDV
                     IYMFSGKKEVDGGWELVIKMVIQNKNSVGTSYMN"
     gene            344..557
                     /locus_tag="ACHK07_005514"
                     /pseudo
     CDS             344..557
                     /locus_tag="ACHK07_005514"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          171 a           89 c          125 g          172 t
ORIGIN      
        1 catgactgaa aggcctaagt gggttagaga acctagttgg cgatttatcg aggataagcc
       61 tatgattttt atttctcagg tggagtttga aaataataaa actatggctg aacatctttc
      121 ttctggtgat gttatttata tgttttcagg gaaaaaagaa gtcgatggcg gctgggagtt
      181 agtaataaaa atggttatac aaaataaaaa ctccgttgga acatcgtata tgaattaaaa
      241 acttcgagtt gttttagttg agtaggtact atgcatcata tccgtgtagt acctccataa
      301 cgggtagtat gatagaccta tttagcaagt ttgtagtcct gacccgatag ggctaaatgg
      361 aggtatcaac ctctatgctt atgcgccgaa tccgctgggt tggatcgatc cgcttggatt
      421 aaatacggaa cctacgttac ccccaggaac tattattaaa gatggtggag taacaataga
      481 gcattattat catggcagtg atcatcttcc tgctcaggga aagatcatca gaagttcact
      541 ttttgtacta aataatt
//
LOCUS       DAZGCQ010000422          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000422 DAZGCQ010000000
VERSION     DAZGCQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.428"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..370
                     /locus_tag="ACHK07_005515"
     CDS             <1..370
                     /locus_tag="ACHK07_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFO8105665.1"
                     /translation="AGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIR
                     LGNKQTYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWR
                     TVSVGEYVSSGASGFEYFPL"
     gene            412..>545
                     /locus_tag="ACHK07_005516"
     CDS             412..>545
                     /locus_tag="ACHK07_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105666.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     V"
BASE COUNT          138 a          110 c          182 g          115 t
ORIGIN      
        1 cgctggtccg cggggagcgc agggaccgaa aggggagaaa ggcgaccccg gtcccagagg
       61 tgagcgtgga gaaaccgggc cgatggggcc gcccggggca tcagacggga agagccgggt
      121 tgtgggtatc aggctgggta ataaacagac ttatgcgccg caaacagaca gcactacatg
      181 ggcagtggat ttggatattg gtgcgatgat tacggggatt ggcggttaca acgacggaaa
      241 caaaaccctt attgacaggg tatcttacag gccgcttcag gtgacatttg atggttctca
      301 gtggcgtaca gtcagcgtgg gtgagtatgt gtcgtcagga gcttcggggt ttgagtattt
      361 tccattataa agcgcatcat ctttcagaca tgtaaccggg aggactgaca gatgcacata
      421 aagaatttca gacaatacac gccggaaaat ccggatgtgc cgggtgcgat gtacctgaaa
      481 tcagaggatg gtcaggactg gtatgagtgt cagtcgttat tttcagcaga gacgctgaag
      541 gtggt
//
LOCUS       DAZGCQ010000423          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000423 DAZGCQ010000000
VERSION     DAZGCQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.429"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..>544
                     /locus_tag="ACHK07_005517"
     CDS             68..>544
                     /locus_tag="ACHK07_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105667.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENGYLASEA"
BASE COUNT          184 a           89 c          137 g          134 t
ORIGIN      
        1 tattcatgca tgatttttat gctattccga ctgagtggaa gaaaataaag gagaaaagag
       61 gatatatatg aacctcagaa aaaaacatat ctgtatagcc atatcacttg ttcttaatcc
      121 gttccctgca tcactggcag cacaaaacac taatgtagtt gaagttgatg aaagcagtca
      181 ggggtatgat gtgcatggag ataactggaa gagcattgaa atagggacaa cgcaggaagt
      241 taaagcatcc cccgataaaa atattaattt cgatcataat tctcctgttg cagaaggacg
      301 taactctatc gcgattggta ccagtgcaga ggctggtaaa gaaatgacgg ataaaatggc
      361 aggggaagcc atagctattg gtgccaaggc gaaagcaact aaagaacagt caattgcaat
      421 aggtggcgat accaaagcta ccggatgggg ggctattgtt attggtgggg atgatttaac
      481 tcctttgcat ggtaagactt atggtcggga aaaaatagag aatggctatc ttgcatcgga
      541 agcg
//
LOCUS       DAZGCQ010000424          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000424 DAZGCQ010000000
VERSION     DAZGCQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.430"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>536
                     /locus_tag="ACHK07_005518"
     CDS             <1..>536
                     /locus_tag="ACHK07_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8105668.1"
                     /translation="KDVIASDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTD
                     EKSNEITAIPELLNMLDIKGKIITIDAIGCQKDIAEKIQKQGGDYLFAVKENQGRLNK
                     AFEEKFPLKELNNSKHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCV
                     AVSFRSIIAEQKKEPEMTV"
BASE COUNT          188 a           91 c          126 g          131 t
ORIGIN      
        1 aaagacgtca ttgcaagtga tggaaaaacg ctccggcatt cttatgacaa gagtcgccgc
       61 aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt catcggacag
      121 atcaggacag atgagaaatc taatgagatc acagctatcc ctgaacttct taacatgttg
      181 gatattaaag gaaaaattat cacaattgat gcgattggtt gccagaaaga tattgcagag
      241 aagatacaaa aacagggagg tgattattta ttcgctgtaa aagaaaacca ggggcggcta
      301 aataaagcct ttgaggaaaa atttccactg aaagaattaa ataattcaaa gcatgacagt
      361 tacgcaatta gtgaaaagag tcacggcaga gaagagatcc gtcttcatat tgtttgcgat
      421 gtccctgatg aacttattga tttcacgttt gaatggaaag ggctgaagaa attatgcgtg
      481 gcagtctcct ttcggtccat aatagcagaa caaaagaaag agccagaaat gacggt
//
LOCUS       DAZGCQ010000425          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000425 DAZGCQ010000000
VERSION     DAZGCQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.431"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..239
                     /locus_tag="ACHK07_005519"
     CDS             12..239
                     /locus_tag="ACHK07_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO8105669.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
BASE COUNT          190 a           93 c          113 g          140 t
ORIGIN      
        1 agataaaaac aatgttcccg gagtacagag aactgatttc agcattgaaa ggaaatcata
       61 aacgctttga ttctttattt gaaaagcata ataaacttga tcacgaaata aaaagaattg
      121 aagatgaatt tggttcagcg tattcgacga aagtggtcag gctgaagctt gaaaaacttg
      181 agatcaaaag agaaatccag aaaatccttg aagatgagtc agaaaagggc gaacggtaat
      241 cataaacggt atattcaaag acgtgttttc tctgttttat gaaaaaggca ccgggggctc
      301 cacggtgcct tttagcctgt gtatagcgaa aaggagatcc tgttcagcga accagatagt
      361 taaaaaaaga atctttacga cggtatcaaa gttttcgtgc aaaaaacatg atcaatatta
      421 aattccagta catccatatc cgatataaat ctttccgtct gacagataca gatatgccac
      481 gtaacgcatc cgctgtttcc ggctgtgccg gaggataata acagtctgta gcttgt
//
LOCUS       DAZGCQ010000426          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000426 DAZGCQ010000000
VERSION     DAZGCQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.432"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /locus_tag="ACHK07_005520"
     CDS             complement(<1..359)
                     /locus_tag="ACHK07_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO8105670.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVIS"
BASE COUNT          144 a          170 c          108 g          107 t
ORIGIN      
        1 ctgctgatca ccccactgta ccccgtctct ccgggctctt caggtgtcca taccccacag
       61 gtctgtgccc tggatttatc gaatgacagc gtatccagcg tcacctgtac cagaggaaca
      121 cagcaggata tcatcacaaa aaaagcacca tagagaacat gctcaatacg cggcagggaa
      181 agcatccctt tgtttccctc atccgcacct tcttccctga ctttcagcca gatcccgatg
      241 atcctgaatg ccagtggcag ggcaaacagt cctgtgctga tcaacacatt ccacagaccg
      301 ttattcacca cccagcccag cagagtcaga aaatattcca gatagttatt tgtggtcatg
      361 attgctcaac gccccggctc agcaaaaatg gcgtattcac acagcaggat aaacagaaca
      421 ctgactgtgg ccatacgcag aaaagaagcc cggtattctg ccctgaaccc cggcgcatgc
      481 cagatcctcc accccagcca tccgatcgca ctgtaaagac acagacgcc
//
LOCUS       DAZGCQ010000427          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000427 DAZGCQ010000000
VERSION     DAZGCQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.433"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..474)
                     /locus_tag="ACHK07_005521"
     CDS             complement(<1..474)
                     /locus_tag="ACHK07_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8105671.1"
                     /translation="MIYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNA
                     YSVVSHNQFFEKVWSDSSAEVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGF
                     KINEEISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVCFFA"
BASE COUNT          156 a           90 c           87 g          194 t
ORIGIN      
        1 cgcaaaaaaa caaactataa gcaatgtatt ctttgtggta aatatacctt ttgcaatgtg
       61 atttttattt atattttcag tgtgggtacc attctcttca ctctcaacac tttcattctc
      121 attgtgatta ttgtcgagaa cagttatgct gatttcttca ttgattttga agcctttacg
      181 gggcaccgtt ctgataacat cattaaatat cgtcgctgag ctgatttcac gaaaagaacg
      241 tcttatcatc gatatatttt gatataaagt gttggttgcg acttcggcac tgctatcgct
      301 ccagactttt tcaaagaatt gattgtgtga cacgacacta taagcgttat ctaaaagtaa
      361 ctttaagcat tttcccgcag gataagtgag tatcacttcc tctgatgtaa ggagtgatgt
      421 taacttatta gatgatgagt tgaatcttac ttcatcattt ataatgtaga tcattacatt
      481 ttatacctta gttgtgtatt acaaatccag tattactcag aaaataa
//
LOCUS       DAZGCQ010000428          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000428 DAZGCQ010000000
VERSION     DAZGCQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.434"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..429)
                     /locus_tag="ACHK07_005522"
     CDS             complement(34..429)
                     /locus_tag="ACHK07_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8105672.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYHRDDDMALWSSADLTYSITYEM"
     gene            complement(426..>524)
                     /locus_tag="ACHK07_005523"
     CDS             complement(426..>524)
                     /locus_tag="ACHK07_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105673.1"
                     /translation="AFKQNIERIRRERLPEELAYALKQQLRIAIKR"
BASE COUNT          120 a          154 c          119 g          131 t
ORIGIN      
        1 ggcgccaggg gatttggtgt tgccatatca tcctcacatc tcgtatgtaa tggaataagt
       61 cagatctgca gagctccaca atgccatatc gtcatcacga tgatactcat agccctgcgt
      121 aaccatcgtg gtaatcatgc ctgccagtgc agggatcgcc gccatcgccg ggtaaatccg
      181 gctttccatc cactgatcaa gctctgaatc cggtacctgt gccggtaaaa acacctcaat
      241 atgcagtgtg gcccgccagg tatcagcatc cagctcttca ccggtatact ctgcatccgt
      301 cagataaacc gcgaccgccg gaaaatccgc ctcctcaaaa acagcggggc gaccatcaaa
      361 cagcgtcgcc ccggtgtcat gcttctccag tgcatccagt acggctgcac ggatatcagt
      421 atgtttcatc gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttct
      481 ggcaggcgtt cacgccggat acgttcaata ttctgtttaa atgc
//
LOCUS       DAZGCQ010000429          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000429 DAZGCQ010000000
VERSION     DAZGCQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.435"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>523
                     /locus_tag="ACHK07_005524"
     CDS             <1..>523
                     /locus_tag="ACHK07_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8105674.1"
                     /translation="DITASAREGKIDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGV
                     GKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQ
                     SPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYRQYLERD
                     AALERRFQMVRVD"
BASE COUNT          110 a          147 c          171 g           95 t
ORIGIN      
        1 gggacatcac ggccagcgcg agagagggga aaattgaccc ggtatcggga cgcgatacgg
       61 aaatccgtca gatggtggat attctctccc gccgccgcaa gaacaacccg attctggtgg
      121 gggaccctgg tgtggggaaa acggctctgg tggaagggct cgccctgcgt attgcggaag
      181 gtaacgtacc ggaatcactc agacccgtca ccctgcgcac ccttgacctc ggcctgctgc
      241 aggccggtgc cggcgtgaag ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg
      301 tgcagcagtc accggctcct gtactgctgt ttatagatga agcccatacc cttatcgggg
      361 ccggtaatca ggccggcggc gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg
      421 gtgaactgcg taccattgcc gccaccacct ggtctgagta cagacaatac ctggaacgtg
      481 acgcagccct ggagcggcgt tttcagatgg tcagagtgga cga
//
LOCUS       DAZGCQ010000430          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000430 DAZGCQ010000000
VERSION     DAZGCQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.436"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>520
                     /locus_tag="ACHK07_005525"
     CDS             <1..>520
                     /locus_tag="ACHK07_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8105675.1"
                     /translation="HDVHVRIPSALTEAALEQIGQLYAIEADIRGMPAEQRLAERQRK
                     TKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENA
                     LRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNR
                     VSELLPWRIALPA"
BASE COUNT          124 a          136 c          161 g           99 t
ORIGIN      
        1 cacgatgtgc acgtccgcat cccgtcagca ctgacggaag cagccctgga gcagatcggt
       61 cagttgtacg ccatagaggc ggatataagg ggaatgccgg cagagcagcg gcttgctgaa
      121 cgtcagcgaa aaacgaaacc gctgttgaaa tccctggaaa gctggttgcg tgaaaagatg
      181 aagaccctgt cgcgacactc agagttggcg aaggcgttcg cgtacgcact taaccagtgg
      241 ccggcactga cgtactatgc gaacgatggc tgggtggaaa tcgacaacaa catcgctgaa
      301 aatgccctgc gggcggtcag tctgggtcgt aaaaacttcc tgttcttcgg ctctgaccat
      361 ggtggtgagc ggggagcgct actgtacagc ctgatcggga cgtgcaaact gaatgacgtg
      421 gatccagaaa gctaccttcg ccatgtgctt ggcgtcatag cagactggcc ggtcaaccgg
      481 gtcagcgaac tgcttccgtg gcgcatagca ctgccagctg
//
LOCUS       DAZGCQ010000431          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000431 DAZGCQ010000000
VERSION     DAZGCQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.437"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          124 a          101 c          126 g          168 t
ORIGIN      
        1 ttgttaaggt tttttagcag gtctacagtc aactccatcg cggtatcctt caggttgtta
       61 tcattcgttg atgatgcgat ctgttgaagg cgttggcgac aattaattct ttttctcttc
      121 tgaaaaattt acggaataca aaaaagatct gtttttaact gattgttact ttctgaagat
      181 attctctgct ccatttgttg aagccggaga gtgttgttta tggcaaaaag ctccatttgc
      241 tttccgggga agacgtatgg ctggttatgg tatggtcgga ctgcagccac agggggatga
      301 ctgtaatacc tctgatcacc acagtcagtc atctgtaaat gttaaatacc tcacagttca
      361 gtcagtggat tattgtcggt cttacgggat atttgttaaa aaaatactgc atggtgaatc
      421 cccctgagcg gcggggcata tcagtatcgt aggtgtttgg tcacatccct tatctgtgcg
      481 ggttcaggtg ctgatactga actcaccggg aggcacccg
//
LOCUS       DAZGCQ010000432          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000432 DAZGCQ010000000
VERSION     DAZGCQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.438"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>515)
                     /locus_tag="ACHK07_005526"
     CDS             complement(<1..>515)
                     /locus_tag="ACHK07_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105676.1"
                     /translation="KLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEGTNGDDSLFGN
                     IIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDWLYGGGGNDYL
                     YGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKGIVTSTDEFISCLKQTGNDV
                     EAGIAGGQIYF"
BASE COUNT          156 a          138 c           63 g          158 t
ORIGIN      
        1 cgaaatatat ctgtcctcct gcaatacctg cctcaacatc atttccggtt tgtttcagac
       61 atgaaataaa ctcatctgtc gatgtaacaa taccctttga taaaaacaga aaatcttttt
      121 cacccttatg tttattaaaa tccatgataa atgaattccc ttcacaagca gcatcaaaga
      181 cgaagacatc atgaccagta ccaccataca gataatcatt acctccccca ccatataacc
      241 agtcgttacc ttctgctccg tacaactcat cattaccaga agccccatga agataatcat
      301 tcccgcgacc tccgtgtaaa agacttactc cttctccccc ggcactatgt aatatatcat
      361 ttccgtcaag accaaaaata atatttccga acagactatc atctccgttt gtaccttctg
      421 tatgttttcc gtaatcaaca tttttattac gccacttcag aagatacctg tggtctgtaa
      481 tctgctcttc ttctatctga taagggctta gttta
//
LOCUS       DAZGCQ010000433          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000433 DAZGCQ010000000
VERSION     DAZGCQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.439"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..489)
                     /locus_tag="ACHK07_005527"
     CDS             complement(31..489)
                     /locus_tag="ACHK07_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105677.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIILLVCIHSLITTIK"
BASE COUNT          172 a           91 c           94 g          157 t
ORIGIN      
        1 tattttcctg tatcagaaca gccataactt ttattttata gttgttatca gtgaatgaat
       61 gcaaactaat agaataatga gaaaaataat ggagaaaaag cgacggaccg gacgccatag
      121 ttcatattct cgtgtatcag cctcacgtat caacctttct gaaatggttt cggtttcttt
      181 tcctcgcacc agaatatgtc tgtaatattt cctcatgaca tataccgaaa actgaaacca
      241 gattaatatg gatggacaca caacacataa acttctgatg atatcacccg ggaaagtatc
      301 ttgggcgggt aaaagaaagt catatactct ggataaaatc atcatcgaga taaatgagac
      361 gccagcacct atccagtatt cgatatgaga agatgctgca ttatatgcgt cagtaaaatt
      421 tttctttctt aatgaaggtg gaagtgctga aagttcagga atgcgttttc ttgtccagta
      481 tattttcata tagtaactca tataaaaagt gttc
//
LOCUS       DAZGCQ010000434          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000434 DAZGCQ010000000
VERSION     DAZGCQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.440"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>509
                     /locus_tag="ACHK07_005528"
     CDS             <1..>509
                     /locus_tag="ACHK07_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO8105678.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRT"
BASE COUNT          125 a          125 c          141 g          118 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtac
//
LOCUS       DAZGCQ010000435          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000435 DAZGCQ010000000
VERSION     DAZGCQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.441"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>496
                     /locus_tag="ACHK07_005529"
     CDS             <1..>496
                     /locus_tag="ACHK07_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8105679.1"
                     /translation="KLLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLR
                     WGVGKPLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQF
                     RTELAGQASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPL
                     MTDEN"
BASE COUNT          116 a          107 c          170 g          103 t
ORIGIN      
        1 aaaactgctg ccgtttatac cggcgaatgc gggcattctg ctggttccgt gctgccgtgg
       61 tgcttcggca ttcacaacgg gggcagacgg cacatacagc gaatcagccg gtgcatcgga
      121 aaattcactg cgctggggtg tgggtaagcc gctgtatcag gatttggtga gccggactaa
      181 agccgcactg gcgaagaacc cgaaaaaccg tctgcttgcg gtggtgtgga tgcagggaga
      241 aggtgatgcg gcagtgggga cgcatgcgca gcatccgggg ctgtttagtg cgatggtgaa
      301 tcagttcaga acggagcttg ccggtcaggc atcacagagc acaggaggca gcgcatcagc
      361 ggtaccgtgg atttgcgggg acacaacgta tttctggaag cagcgtcatg cggaagggta
      421 tgcatcagta tacggagggt ataagggtaa ggagtcgcag aacattttct ttgtaccgtt
      481 aatgacggat gagaat
//
LOCUS       DAZGCQ010000436          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000436 DAZGCQ010000000
VERSION     DAZGCQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.442"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHK07_005530"
     CDS             34..>482
                     /locus_tag="ACHK07_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105680.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          126 a          108 c          151 g           97 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggcga taaaagggct tgatcaggcg
       61 attgataatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgcgtgg ccacaacggc aataaatcag tctgcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt aaaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgcag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZGCQ010000437          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000437 DAZGCQ010000000
VERSION     DAZGCQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.443"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHK07_005531"
     CDS             34..>482
                     /locus_tag="ACHK07_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105681.1"
                     /translation="MVIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          128 a          106 c          150 g           98 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggtga taaaaggact tgatcaggcg
       61 attgacaatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgtgtgg ccacaacggc aataaatcag tcggcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagagcc aggctgaaaa gggccacggt taaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgaag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZGCQ010000438          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000438 DAZGCQ010000000
VERSION     DAZGCQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.444"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>476
                     /locus_tag="ACHK07_005532"
     CDS             <1..>476
                     /locus_tag="ACHK07_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105682.1"
                     /translation="PTEKDISKINSSDKPYKTSSKESIKSQLYSDQKNIQTCYYILRK
                     NTEWYKYASSEERYDKFKNSSKEIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKK
                     LAPLINDSFSYTRDFFAYSKQEKNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGK"
BASE COUNT          208 a           80 c           72 g          116 t
ORIGIN      
        1 ccgacagaaa aagacatttc aaaaataaac agctctgata aaccttataa aaccagcagt
       61 aaggaaagta ttaaaagtca actttacagc gatcaaaaaa atatacagac ctgctactac
      121 attctgagaa aaaatacaga atggtacaaa tacgcatcct ccgaggaacg ctatgataaa
      181 ttcaagaatt cttctaagga aattaaaaat acctacaagc aaattgtaca agcgcagaaa
      241 aaattgaacc aaatgaaata catcaataaa ttaagtggtg agcttatcga tatcaccgac
      301 aaaaagttag caccattaat aaatgatagt ttcagctata cacgagattt ctttgcttac
      361 tcaaaacagg aaaaaaatat cttcacattt gataattcta agtttgtaga tccaaaggaa
      421 aaagaaggtt taatggtaca acacagcaat gggcaactgg ttattactgg taaata
//
LOCUS       DAZGCQ010000439          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000439 DAZGCQ010000000
VERSION     DAZGCQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.445"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..96
                     /locus_tag="ACHK07_005533"
     CDS             <1..96
                     /locus_tag="ACHK07_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO8105683.1"
                     /translation="VLGSLLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            100..>472
                     /locus_tag="ACHK07_005534"
     CDS             100..>472
                     /locus_tag="ACHK07_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105684.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSTLMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGGKITISGNGKVTPPSHQ
                     HSEELIEFAIDYLKNNKKQGLM"
BASE COUNT          146 a           88 c          126 g          112 t
ORIGIN      
        1 gtgctgggga gtctgctgtt tgggctgctg acatatctga cgaacctgta tttcaaaatc
       61 aaagaggacc ggcgtaaggc cgcccgggga gagtgaataa tgaaccatga agaaatgaat
      121 cagcgcttca gtcgcctgga aaatgaaatt gctgaactga ataaaaaact gtcgacgctg
      181 atgccttctg aagatgaaaa aaaacgccgc gatgagcagt ttgctgcgtt tgacgattat
      241 tgtcggaaag tgatgagcag aaatctcgca gagtgtttca gtattcataa tgataatttc
      301 agtgagctgg aatgggagtg taaccggcca tcctttgttg tatccggtga tggtgggaaa
      361 ataaccatct caggaaatgg gaaagtaaca cctccatcgc accagcacag tgaggagctc
      421 attgaatttg ccattgatta cctgaagaac aataaaaaac aggggctgat gc
//
LOCUS       DAZGCQ010000440          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000440 DAZGCQ010000000
VERSION     DAZGCQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.446"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..78
                     /locus_tag="ACHK07_005535"
     tRNA            2..78
                     /locus_tag="ACHK07_005535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Arg,seq:acg)
     gene            277..353
                     /locus_tag="ACHK07_005536"
     tRNA            277..353
                     /locus_tag="ACHK07_005536"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:311..313,aa:Arg,seq:acg)
BASE COUNT          129 a          118 c          129 g           93 t
ORIGIN      
        1 tgcatccgta gctcagctgg atagagtact cggctacgaa ccgagcggtc ggaggttcga
       61 atcctcccgg atgcaccata ttctccgaaa tcttcagcaa tgaagatatc gaagaggtag
      121 cggaattaac cgcaggcacc agggaggata acgttgcgcc agcaacggcc cgaagggcga
      181 gccgcaaggc gagtaatcct cccggatgca ccatctctta cttgatatgg ctttagtagc
      241 gatatcaata tcagcagtaa aataaatttt cccgatgcat ccgtagctca gctggataga
      301 gtactcggct acgaaccgag cggtcggagg ttcgaatcct cccggatgca ccatattatc
      361 cgaaatcttc agcaatgaag atatcgaaga ggtagcggta ttaaccgcag gcaccaggga
      421 ggataacgtt gctttagcaa cggcccgaag ggcgagccgc aaggcgagt
//
LOCUS       DAZGCQ010000441          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000441 DAZGCQ010000000
VERSION     DAZGCQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.447"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHK07_005537"
     CDS             complement(<1..150)
                     /locus_tag="ACHK07_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105685.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNE"
     gene            complement(236..421)
                     /locus_tag="ACHK07_005538"
     CDS             complement(236..421)
                     /locus_tag="ACHK07_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO8105686.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          112 a          126 c          111 g          119 t
ORIGIN      
        1 ttcattcgtg gcattcacct gaaaggtccg ggagtgtaat tgcgtacatt taccactgaa
       61 cgaaccttca acaagaacac gaccacgctg caaaatacgg aacggaattg ttccctgaaa
      121 aggttctacg gttaccagta atttcttcat gcattctccg gataacaaaa atactagtta
      181 atacactgag tgcggatata ttccttcgcc ccttccagct gcttctgcat tgtcatcaac
      241 cgttctctga ggatgaaata atcccgttca gcggtgtctg ccagtcgggg gccggttgca
      301 ttatccacgc cggaggtgct ggtggcttca cgcacggtac cgtggcaggt ggcgttgatc
      361 cgcaggcgct tacgaccagc ggcaacatca gcacgcagag tttcattttc agctctcgca
      421 tcggctaatt ccctcgagta ttttgcatcg agcgcagcaa catcgagc
//
LOCUS       DAZGCQ010000442          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000442 DAZGCQ010000000
VERSION     DAZGCQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.448"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>467)
                     /locus_tag="ACHK07_005539"
     CDS             complement(<1..>467)
                     /locus_tag="ACHK07_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8105687.1"
                     /translation="GATGGKALQLNKPESHIWFLEHDAAGQGAELLKKGGRVSVRFKV
                     PGSLVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQNAKL
                     DTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQL"
BASE COUNT           92 a          153 c          111 g          111 t
ORIGIN      
        1 cagttgcagt ttgtccgccg cccctgatgc cggagatttc accagtgtga acggtgtacc
       61 ccgtttctca tccagtaccg gcgtcacctg aatgctgttg tttccggcaa actcaaaagc
      121 cagtgtgtgc catccgttat caaagacccc gaacgtatcc agcttcgcat tctgcttctt
      181 gtggtacatc gcgttcaggt tcgtcgcatc cgtctgcagg aagaaggaca tcagcatgtc
      241 gttgccttcc tctgacagcg tcactccctc cggcagggac gacaactgcc agtaaatacc
      301 cagggcaaac tgattcggca ccagtgagcc cggcacctta aaccgtacgc tcacacgtcc
      361 gcctttcttc agcaactctg ccccctgccc ggcggcatca tgctccagaa accagatgtg
      421 gctttccggt ttgttcagtt gcagggcctt accaccggtg gcaccct
//
LOCUS       DAZGCQ010000443          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000443 DAZGCQ010000000
VERSION     DAZGCQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.449"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>463
                     /locus_tag="ACHK07_005540"
     CDS             <1..>463
                     /locus_tag="ACHK07_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO8105688.1"
                     /translation="KIALSINDKKYNVNSKDIENILDGKGDLFKKRTLWEFVRDLFPG
                     SHIKEVKGLIYEFVTKVDNKAEVFDKIKSLAKTEQQWRFSTKTDFTTNENNEVIVSRS
                     FNLYTGATSNDNEKKQVSSERLTLDNYLDDSHFDNSPLNRLTFDDYSVKLTT"
BASE COUNT          168 a           65 c           85 g          145 t
ORIGIN      
        1 aaaattgctt taagtattaa tgataagaaa tataatgtaa actctaagga tattgaaaat
       61 attcttgatg gcaaagggga tttatttaaa aaacgtacac tgtgggagtt tgttcgcgat
      121 ctttttcctg gatctcatat taaagaagtc aaaggcttaa tttacgaatt tgtcacgaaa
      181 gttgataaca aagccgaggt gttcgataag attaaatcgc tggcaaaaac agaacaacaa
      241 tggcgattta gtactaagac agactttacc acaaatgaaa ataatgaagt tattgtgagt
      301 agatccttta atctctacac gggggctact tcgaatgata acgaaaaaaa acaggtttct
      361 tcagagagat taacgcttga taattatctt gatgattcac attttgataa ttctcccttg
      421 aatagattaa cgtttgatga ttattctgta aaacttacga cgc
//
LOCUS       DAZGCQ010000444          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000444 DAZGCQ010000000
VERSION     DAZGCQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.450"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHK07_005541"
     CDS             <1..285
                     /locus_tag="ACHK07_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105689.1"
                     /translation="NPSGLYSPRNILEMIAGRAPKFTARVKVIKTGKIETRDIPYELH
                     HKNIPQRQGGPGVHNKSNLDEVDPWQHQDTDKYRHSGIELIEVITGVNSW"
BASE COUNT          164 a           76 c           88 g          127 t
ORIGIN      
        1 aatccatcag gattatattc accaagaaat attttagaaa tgatcgctgg gcgtgcacct
       61 aaattcacag ctagagttaa agtaattaaa acgggtaaaa tagagacaag agatatacca
      121 tatgagttac accataaaaa cattcctcaa aggcaaggtg ggcctggtgt tcataataag
      181 agtaatttgg atgaagtcga tccttggcaa catcaggata cggataaata cagacactca
      241 ggaattgaac ttattgaggt tataacagga gttaactcat ggtaattgat aataactata
      301 ttttattcgg tgcggcattg tccgagatag cgacgaattc atataatgct ggtatcggtc
      361 acaaagagtc agagttgttt ataaaacgta tttatgatga atatttacta acatctaatc
      421 ctgcaccaga cattacctgg ataaaatcca ttccc
//
LOCUS       DAZGCQ010000445          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000445 DAZGCQ010000000
VERSION     DAZGCQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.451"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..454
                     /locus_tag="ACHK07_005542"
                     /pseudo
     CDS             229..454
                     /locus_tag="ACHK07_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          139 a           93 c          122 g          100 t
ORIGIN      
        1 aattccaaat acaaattctg gagacattga taatattggt gagtgtcaaa ttttcatcag
       61 gtataaacac agctggattg agaataaatt caaagaaaaa ataattgaat aaccatgtta
      121 tgtatagaca ttttagatgg aacgaaccag aggaaggcat gcctccgact tgggttatat
      181 gggcacggag ttttagtgta gtgaactcaa cgacgcaaac cgaataacct gccgctggcg
      241 cttatcagca cggaaggggc aacagaatgg tgcgcagaat acgatgagtg gggcaacctg
      301 ctgaatgaag agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat
      361 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg
      421 tatatcactc aggatccgat tgggctgaag gggg
//
LOCUS       DAZGCQ010000446          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000446 DAZGCQ010000000
VERSION     DAZGCQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.452"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>453
                     /locus_tag="ACHK07_005543"
     CDS             <1..>453
                     /locus_tag="ACHK07_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105690.1"
                     /translation="SVVSLAAGGISFALTASIFWLSTGDRDAALQTAAVQAGKTFTRT
                     LAVYVTTQQLHRLTVVQGMLKHIDFSTASPTVRQALQKGTGAGNISALNKVMKGTLVT
                     SLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVAGGI"
BASE COUNT           89 a          123 c          141 g          100 t
ORIGIN      
        1 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc gatcttctgg
       61 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      121 ttcacccgca cactggctgt ctacgtcaca acccagcaac ttcaccggct cactgttgtt
      181 cagggtatgc tgaagcatat tgatttttcg acggccagcc cgactgtccg gcaggcgctt
      241 cagaagggga ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggtacgctg
      301 gtgacatctc tggcactggt agctgtcaca accggccctg acatgatcaa aatgttgcgg
      361 ggacggatct ccggtgcgca gttcatcagg aatcttgccg tggcatcttc cggtgtggca
      421 ggtggtgctg tcgggtcagt ggcgggcggg ata
//
LOCUS       DAZGCQ010000447          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000447 DAZGCQ010000000
VERSION     DAZGCQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.453"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>453
                     /locus_tag="ACHK07_005544"
     CDS             <1..>453
                     /locus_tag="ACHK07_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105691.1"
                     /translation="SVVSLAAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRT
                     LAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNISALNKVMKGTLVT
                     SLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGAVGSVAGGI"
BASE COUNT           89 a          125 c          138 g          101 t
ORIGIN      
        1 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc ggtcttctgg
       61 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      121 ttcacccgca cactggctgt ctacgtcaca acccagcaac tccaccggct cagtgttgtt
      181 cagggtatgc tgaagcacat tgatttttcg actgccagcc cgactgtccg actggctctt
      241 cagaagggga ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggtacgctg
      301 gtgacatctc tggcactggt cgctgtcaca accggccctg acatgatcaa aatgttgcgg
      361 ggacggatct ccggtacgca gttcatcagg aatcttgccg tggcatcttc cggtgttgca
      421 ggtggagctg tcgggtcagt ggcgggcggg ata
//
LOCUS       DAZGCQ010000448          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000448 DAZGCQ010000000
VERSION     DAZGCQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.454"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..102
                     /locus_tag="ACHK07_005545"
     CDS             <1..102
                     /locus_tag="ACHK07_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105692.1"
                     /translation="LYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
     gene            99..>445
                     /locus_tag="ACHK07_005546"
     CDS             99..>445
                     /locus_tag="ACHK07_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105693.1"
                     /translation="MIIINLRLTLLFILATFTYSVQSDEFDDQTTYTQYRYFSTAIPE
                     DIRRFMFYVDYCFVDLAFDIETDSMTAKNDIINECTPLKERNKTLKEKYKKNEEIIAI
                     LSITDDTILIYEE"
BASE COUNT          178 a           71 c           57 g          139 t
ORIGIN      
        1 ctatatccat gctatactga gttaaacaaa ataagagcca tgcaactaaa caatgcttac
       61 atttctgact ttcaaaatcc agatgagata tccagcaaat gattattata aatttaagat
      121 taacattact atttatactg gccacattta catattcagt acaatcagac gaattcgatg
      181 atcaaaccac ttacacgcaa taccgttatt tttccactgc tataccggaa gatatacgta
      241 gatttatgtt ctacgtagat tattgctttg tagatttagc attcgatatc gaaactgact
      301 caatgacagc taagaatgat attattaatg aatgtactcc attaaaagaa agaaacaaga
      361 cattaaaaga aaaatataaa aaaaatgaag agataatagc aattctttcc attacagatg
      421 atactatttt aatttatgaa gaatt
//
LOCUS       DAZGCQ010000449          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000449 DAZGCQ010000000
VERSION     DAZGCQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.455"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..>443
                     /locus_tag="ACHK07_005547"
     CDS             55..>443
                     /locus_tag="ACHK07_005547"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8105694.1"
                     /translation="MNNKIIACMVVKNEVDIIEYTLEQALKWADYILVLDNNSTDGTT
                     DVIKKMSELEQKIIFWGGYGGPFRDSLRAVIYNDYYYLCNDGDWWCRLDADEVYIDNP
                     REFLSSMDESIDHVYSASFQYYLTKRM"
BASE COUNT          150 a           46 c           97 g          150 t
ORIGIN      
        1 taattgggtg aaaaatagac tcgctgaatg aaatgtattt aaaaggagct cttcttgaat
       61 aataaaataa ttgcctgcat ggtagttaaa aatgaagttg atataattga gtatacactt
      121 gagcaggcat taaaatgggc tgattatatt cttgttttag ataataatag tactgatggt
      181 actactgatg tgataaaaaa aatgagtgaa cttgagcaaa aaattatttt ttgggggggc
      241 tatggaggac catttaggga ttcgctcaga gcagttatct ataatgacta ttattattta
      301 tgtaatgatg gtgattggtg gtgtagatta gatgctgatg aggtctatat tgacaatcct
      361 agggaatttc tatcatctat ggatgaaagc atagaccatg tttattcagc aagctttcag
      421 tattatctta cgaaaagaat gtt
//
LOCUS       DAZGCQ010000450          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000450 DAZGCQ010000000
VERSION     DAZGCQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.456"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..362
                     /locus_tag="ACHK07_005548"
     CDS             <1..362
                     /locus_tag="ACHK07_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO8105695.1"
                     /translation="EYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDY
                     HTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKY
                     NPKAKGNGVTIIRQGSN"
BASE COUNT          170 a           56 c           89 g          123 t
ORIGIN      
        1 aagaatatgg tggactaata tgtaagaagc aaggtgaata tttccccatg aatccgataa
       61 gttcaaatga taatgatagt gtagacttgc gaaatataaa atgccctgaa ggttcagaga
      121 gagtaggtga ttatcacact cacggttttt actctgacga taaaggaaat aaagtaacaa
      181 aagaaaatga tgtttatgat agtctaaatt tttcaagcaa agatttaacg aattcttata
      241 tgaatggaat gggaaaaaaa gaatacagta gttacttggg aacaccaaat aacacctatc
      301 taaaatataa tcccaaagct aaagggaatg gagttacaat tatcaggcaa gggagtaatt
      361 aaaatgaagg caatacaatg ttgttatttc cttgctttag tctccagtca tttaatggct
      421 ggagttatga gtgatgac
//
LOCUS       DAZGCQ010000451          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000451 DAZGCQ010000000
VERSION     DAZGCQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.457"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>435)
                     /locus_tag="ACHK07_005549"
     CDS             complement(<1..>435)
                     /locus_tag="ACHK07_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO8105696.1"
                     /translation="DMLRARVTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEI
                     SVTSQTAGISVVTASINSSSQSRDVTFIADIRTAQIADLVVIKDGSVADGAMANTLWV
                     RVTDAFGNALAGQTVSVLADNGATTAPTVTTQPDGTVEISVT"
BASE COUNT           69 a          157 c          115 g           94 t
ORIGIN      
        1 ctggtgacac tgatttccac cgtgccgtcc ggctgcgtgg tgaccgtcgg agctgtcgtg
       61 gcaccgttgt ccgccaacac gctgaccgtc tgcccggcaa gcgcattacc gaacgcatcc
      121 gtgaccctca cccacagcgt gttcgccatt gcgccgtctg ccactgagcc gtccttaatg
      181 accaccaggt ccgcgatctg cgccgtcctg atatcggcga taaacgtcac atcccggctt
      241 tgactgctgc tgttgatgct ggcggtgacc acactgattc ctgccgtctg gctggtgaca
      301 ctgatctcca ccgtgccgtc cggctttgtg gtcatcgtcg aagcgaccgc tgcaccgttg
      361 tccgccatca cgctgaccgt ctgcccggca agcgcattac caaacgcatc ggtgaccctc
      421 gcccgcagca tatcc
//
LOCUS       DAZGCQ010000452          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000452 DAZGCQ010000000
VERSION     DAZGCQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.458"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>435
                     /locus_tag="ACHK07_005550"
     CDS             <1..>435
                     /locus_tag="ACHK07_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO8105697.1"
                     /translation="GYRGGKLDLNGYDFTFSRLQAADYGAEISNDNQTDKSIVTLSLS
                     PLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYYLLKSNRYGSALFGALNNQSEWQ
                     RLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNTSVEIPKL"
BASE COUNT          147 a           69 c          103 g          116 t
ORIGIN      
        1 ggatatcggg gggggaaact ggatttaaat gggtatgatt ttaccttttc ccgtcttcag
       61 gctgcagatt atggtgctga aatcagcaac gataatcaga cagataaatc catagtcaca
      121 ctttcgttat ctcctctgaa agcagaagaa ataaatgtgg ttgttaataa tataaatata
      181 atggggggga caggcaaacc aggtgatctg tattatacga cctttgacgg aaattattat
      241 ctgttgaaaa gtaaccgata tggcagcgct ttgtttggcg cgctgaataa tcagagcgaa
      301 tggcaaaggc tgggtaagga taaagaaaaa gcaattgggt tatatactca gatgaaaatg
      361 caggaaagcg ctcctttatc atatatatat catggaaaaa taaccggtaa taccagtgtg
      421 gaaatcccca aactg
//
LOCUS       DAZGCQ010000453          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000453 DAZGCQ010000000
VERSION     DAZGCQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.459"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>432
                     /gene="rfaY"
                     /locus_tag="ACHK07_005551"
     CDS             <1..>432
                     /gene="rfaY"
                     /locus_tag="ACHK07_005551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO8105698.1"
                     /translation="VLKVYAPKHKMIERFLKSCIKKDYYENLIYQTDRVRGEGIQSIN
                     DYFLLAERKTLNFAHYYIMLIEYIEGIGLNEYLEISEELKDQLSESIKELHQHGMVSG
                     DPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYN"
BASE COUNT          158 a           59 c           80 g          135 t
ORIGIN      
        1 tgtacttaaa gtttatgcgc caaagcataa aatgatcgaa cgtttcttaa aatcttgcat
       61 aaaaaaagat tattatgaaa atttaattta tcaaaccgat cgggtacgtg gtgaaggcat
      121 tcaatcaatt aatgactatt ttttgcttgc tgaacgtaaa acattaaatt tcgcgcatta
      181 ttatattatg ctcattgagt atattgaagg tatcgggtta aatgagtatt tggaaatttc
      241 tgaagaactg aaggatcaat tatcagaatc gataaaagag ttgcatcagc atggaatggt
      301 gtctggcgat cctcataaag gaaattttat agtatccgaa aaaggtttaa gactaattga
      361 cctttcaggt aaaaaaacaa ccgcagtttt gaaagcaaaa gatcgcattg atcttgaacg
      421 gcactataat at
//
LOCUS       DAZGCQ010000454          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000454 DAZGCQ010000000
VERSION     DAZGCQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.460"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..288
                     /locus_tag="ACHK07_005552"
     CDS             <1..288
                     /locus_tag="ACHK07_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105699.1"
                     /translation="KSSAFIISNYPPFKNKVLLILSATSPVQLVILSFDQCLNVEVLF
                     DSMSPVFAKYREDISATLPLWKVILTKNDIELYEYHRTNPYSLNKLLSMSK"
BASE COUNT          142 a           73 c           75 g          141 t
ORIGIN      
        1 aaatcatcag catttatcat atctaattac ccaccattta aaaacaaagt tctgttaatt
       61 ttatccgcta ctagtcccgt tcagttggtt atattgtctt ttgatcaatg tcttaatgtg
      121 gaggtgttat ttgattcaat gtctccagtt ttcgctaagt atagagaaga tattagtgca
      181 acattaccat tatggaaggt tattttgaca aaaaatgata tagaattata tgagtaccat
      241 agaacaaatc catactcact taacaaatta ttgagcatgt caaaataacg gtcaagtatt
      301 caacgttgta atattattcc ttttgctgag tgtctcgaac ggctaaagtt gacgtaaaat
      361 gccacgtttt gtgggcaaac cgaatcggtt aaaaagcatg gtccgggaaa agctaaacat
      421 cagcgctatc g
//
LOCUS       DAZGCQ010000455          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000455 DAZGCQ010000000
VERSION     DAZGCQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.461"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(316..>422)
                     /locus_tag="ACHK07_005553"
     CDS             complement(316..>422)
                     /locus_tag="ACHK07_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105700.1"
                     /translation="VILIISVLMAIGIMRIIHTLKDIELFFTGDKRRK"
BASE COUNT          101 a           85 c           96 g          140 t
ORIGIN      
        1 atatgtgcac gctcggcttt tcttgcggcc atgctgatat gcgcatgatg gttagctggt
       61 ctggcggtta tttctctgcc gggtgattcg gctgttctca tttgttgact cctaaaaaaa
      121 agacaaaggg aacccgaagg catgaatgcc ttaattaatt gctcgtgtgt tttctggtgt
      181 tattgcgttc ttgtttgcag cttcagttta tccacagatt ttcagggcta accaggtgag
      241 gtcacacacc ataaatatca cccatacagt ggcgatggtg agtattgctt ttacattctt
      301 cataaagttt ttgctttatt tacggcgttt atcgccggta aagaataatt cgatatcctt
      361 cagtgtgtgg ataattcgca taatgccgat tgccataagc acggagataa tcagaattac
      421 cc
//
LOCUS       DAZGCQ010000456          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000456 DAZGCQ010000000
VERSION     DAZGCQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.462"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>420)
                     /locus_tag="ACHK07_005554"
     CDS             complement(<1..>420)
                     /locus_tag="ACHK07_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8105701.1"
                     /translation="YNNGYPTTYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRS
                     RRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPK
                     LAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIK"
BASE COUNT           99 a          113 c          110 g           98 t
ORIGIN      
        1 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
       61 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
      121 atgcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg
      181 atggccacgg gattggtgca ccgactggat aaaactctgc aggaggatga gccgaggtgt
      241 aaagctgcgc ccacggcgac cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg
      301 ccggagcatg agcaccgctt gtaccactcc agccgatgag taactcacct tcgccaacgg
      361 ctgtcatccc tttcaggtga atgatatttc catacgttgt tggatatccg ttgttataca
//
LOCUS       DAZGCQ010000457          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000457 DAZGCQ010000000
VERSION     DAZGCQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.463"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>412
                     /locus_tag="ACHK07_005555"
     CDS             <1..>412
                     /locus_tag="ACHK07_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO8105702.1"
                     /translation="TSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPLE
                     EALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYS
                     PDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGR"
BASE COUNT           82 a          110 c          132 g           88 t
ORIGIN      
        1 tgacctcgaa gtatgcagag cacaccccgc tgtatcgcca gtcagaaata tacggccggc
       61 aaggtgtgga gctgagccgt tcactgctgt cgggctgggt ggatgcatgc tgccggctgc
      121 tgtctccgct ggaagaggcg cttcatggct atgtcatgac tgacggcaaa ctccatgccg
      181 atgatacccc ggtccaggta ctgctgccgg gtaataagaa gacgaagacc gggcggttgt
      241 gggcgtatgt tcgtgatgac cgcaatgccg ggtcagcgtt ggcacctgca gtgtggttcg
      301 cttacagccc ggacagaaaa ggcatccatc cgcagactca tcttgcttgc ttcagcggtg
      361 tgctgcaagc ggatgcgtac gccgggttca acgagctgta tcgcaatggt cg
//
LOCUS       DAZGCQ010000458          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000458 DAZGCQ010000000
VERSION     DAZGCQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.464"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..396
                     /locus_tag="ACHK07_005556"
                     /pseudo
     CDS             1..396
                     /locus_tag="ACHK07_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          111 a           87 c          112 g           86 t
ORIGIN      
        1 caaagaagcg gtggagtatg gtcagtcgcg aaaatggata gcgcatgcgc ccttgttgcc
       61 aacggacaag gaataaccct ggtaagccgt attctgggcg tgtcgcgtgc gcaactggca
      121 caacggatga aaacatctgg taataagccg aataaacgtc gtcgcagatg tgatgaggct
      181 gcggatgcgg aagtgttagc acgtatcctt gatatcatca gcgatatgcc gacatatggc
      241 tatcgtcgtg tctgggccat tctcagacga cagtcccgta acaaaggagt gccgttcgtg
      301 aatgcaaagc gcgtttatcg gataatgagc gaaaataacc tgttgttgtt acatgacaaa
      361 ccttcacggc cgcagcggga acataaaggt cgaata
//
LOCUS       DAZGCQ010000459          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000459 DAZGCQ010000000
VERSION     DAZGCQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.465"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>390
                     /locus_tag="ACHK07_005557"
     CDS             <1..>390
                     /locus_tag="ACHK07_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105703.1"
                     /translation="PDNIVLYTDLYGSFLWNIIPFSVLALPLGSWSAHFAGSKTDAFE
                     RILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVHDKGDESRERPVFIIGNDGNNLLAGG
                     KGGDYIEGGGGNDKIEPGKGANRVDGGS"
BASE COUNT          102 a           69 c          115 g          104 t
ORIGIN      
        1 cctgataaca ttgttttata cacagacctt tatggttcat tcctctggaa tattatcccc
       61 ttttctgtgc ttgcactccc gctgggaagc tggagtgcgc attttgcggg ttcaaaaact
      121 gacgcctttg aaagaatttt aggggctcag ttttacgact acatgtcacc ggacagtaca
      181 gtcattgtgg atgagcaaag tagctggaca tcctggtatt catgggttca tgacaagggg
      241 gatgagagtc gtgaaagacc tgtattcatc atcggtaatg atggcaataa tctgctggca
      301 ggcgggaagg gtggcgacta tatagaggga gggggaggga atgataagat tgaaccaggt
      361 aagggagcaa acagggttga tggtggcagt
//
LOCUS       DAZGCQ010000460          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000460 DAZGCQ010000000
VERSION     DAZGCQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.466"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHK07_005558"
     CDS             <1..254
                     /locus_tag="ACHK07_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO8105704.1"
                     /translation="QLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSV
                     QRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            238..>389
                     /locus_tag="ACHK07_005559"
     CDS             238..>389
                     /locus_tag="ACHK07_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO8105705.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDA"
BASE COUNT           99 a           83 c          130 g           77 t
ORIGIN      
        1 agcaactggt ggcggaagag ctggtggaga agtgggaaaa aggaaagatg cgtctgctgt
       61 gggataacaa aaagcggcgt aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat
      121 tacgtgtgtc cgtgcaacgc tggcagcttg atctggctgt actggcaaaa tcccgggaag
      181 aagagacgac ccggccaacc ctgaaagaac tggcagcgaa gctgtccgga ggagtgaatg
      241 gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa
      301 cgggtggtgt ctgtccagaa agatggtcgc agaattgaat atacggcggc ttctctggat
      361 gagcttaacc gtgcgatcaa tgatgcgga
//
LOCUS       DAZGCQ010000461          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000461 DAZGCQ010000000
VERSION     DAZGCQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.467"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>387
                     /locus_tag="ACHK07_005560"
     CDS             <1..>387
                     /locus_tag="ACHK07_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO8105706.1"
                     /translation="ASASETDSTAVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYV
                     NPEASNGVAVGSWASADALNSVALGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGV
                     TNPASPRVPDKTRVLGGVSDGTRDTDA"
BASE COUNT           85 a           92 c          123 g           87 t
ORIGIN      
        1 gcctctgcat cagagactga ctccacggca gtgggggcat gggcgaatgc cgggaaggca
       61 gggacagctc ttggctctgc atcgtatgcg catgcgccgt tttccacatc cgtggggatg
      121 tctgcttatg taaatccgga ggcgtctaat ggtgtggcag taggtagctg ggcatctgca
      181 gatgctttaa attcagtagc actgggtacg ggtgcacagg caatgcacag gtattcagtg
      241 gctcttggtc agcagtccgg tacaacccgt gaaaatgaag tctacatcgg atatgactca
      301 ggtgtcacaa acccggccag tccgcgtgta ccggataaaa cccgggttct cggtggtgtc
      361 agtgatggta cacgggatac ggatgca
//
LOCUS       DAZGCQ010000462          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000462 DAZGCQ010000000
VERSION     DAZGCQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.468"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>386
                     /locus_tag="ACHK07_005561"
     CDS             <1..>386
                     /locus_tag="ACHK07_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO8105707.1"
                     /translation="SLDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDL
                     QYFKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLES
                     QAIETLSAIGLSYEKYKTANNVYFNI"
BASE COUNT          138 a           63 c           74 g          111 t
ORIGIN      
        1 tagtttggat gaagcagtct gggtcaaaca agcactggaa aaaaatcaac caggaaagcc
       61 agtacgggtg ataaatcctg atgatgaatc gatacgaatt ttttcatggc ttgctgataa
      121 tttccctgat ttacaatatt ttaaacttca actattagat gccagtaatc caagattgac
      181 agtgagtaag caacggaacg ctatcacaca gcaactaatc gataatctta ttaaaggatt
      241 actgcaaact atgccatatg ccagcaatat aagtattgcg gtattagatg ataatgtatt
      301 ggaaagtcag gctattgaaa cgttgtcggc gataggtctt tcttatgaaa aatataaaac
      361 agctaacaat gtgtatttca atatca
//
LOCUS       DAZGCQ010000463          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000463 DAZGCQ010000000
VERSION     DAZGCQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.469"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          171 a           70 c           56 g           87 t
ORIGIN      
        1 cggagcaccg gcaatcagaa aggaaataag cgataaagta ataccgtgtc gatttatttt
       61 cataaacaac ctcattgata tacacaatat actggcacag agttaaatta atatataatc
      121 aggagagaag tgaaagagta cccggtgcga aaatgaatta atctgagcag atttaacggt
      181 aaacgaagcg caataaatta aaattaaaaa acaaaatcac ttgcagtcta ttatctttac
      241 gttcctcacc gcaaccgcac acaaaaacaa aacacacaat gcattgattt taaacaaaaa
      301 aaacaaaaca gaaactcaac acataaaaat cagacaatat aaacagaatt gtgatttata
      361 tcctcaagcg ctgtattaac gaaa
//
LOCUS       DAZGCQ010000464          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000464 DAZGCQ010000000
VERSION     DAZGCQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.470"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..225)
                     /locus_tag="ACHK07_005562"
     CDS             complement(<1..225)
                     /locus_tag="ACHK07_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105708.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQ"
BASE COUNT           69 a          123 c           79 g           96 t
ORIGIN      
        1 ctgctcctgc tccccggcac gccagacggc ggtcacaccg tgtatcacgg gattaccgtc
       61 cgtgtccgtc agcggggttt tgttcaccag aatactctgc agtcccttca ccggaccttc
      121 caccggtccc tcaccaatgg catcaatcac gctcatcatc tgcgtggatt tgagattgtc
      181 cttcgcctca cgcggtgtgt gccccttgcc gcccccttta cccactctgt ccccctctcc
      241 tgtctgatgt ctgaatctgt ttatgccaga aaacaacagg caccccggag ggtgcctgtg
      301 tcatgacgga ataaaatttc tgaaattctt cacatttccg gcaattgcct gtagccacta
      361 taatgac
//
LOCUS       DAZGCQ010000465          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000465 DAZGCQ010000000
VERSION     DAZGCQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.471"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>367
                     /locus_tag="ACHK07_005563"
     CDS             <1..>367
                     /locus_tag="ACHK07_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO8105709.1"
                     /translation="DALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTL
                     NDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLS
                     HAGQIHFTSTRTGKFVPATL"
BASE COUNT           89 a          121 c           94 g           63 t
ORIGIN      
        1 gcgatgcact cctgcaggga ggctctctca ccggtaacgg cagcgtggaa aaatcaggaa
       61 gtggcacact cactgtcagc aacaccacac tcacccagaa agccgtcaac ctgaatgaag
      121 gcacgctgac gctgaacgac agtaccgtca ccacggatgt cattgctcag cgcggtacag
      181 ccctgaagct gaccggcagc actgtgctga acggtgccat tgaccccacg aatgtcactc
      241 tcgcctccgg tgccacctgg aatatccctg ataacgccac ggtgcagtcg gtggtggatg
      301 acctcagcca tgccggacag attcatttca cctccacccg cacagggaag ttcgtaccgg
      361 caaccct
//
LOCUS       DAZGCQ010000466          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000466 DAZGCQ010000000
VERSION     DAZGCQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.472"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>364
                     /locus_tag="ACHK07_005564"
     CDS             <1..>364
                     /locus_tag="ACHK07_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105710.1"
                     /translation="SCVALSACSAQEIAELNRQISDGAFAVTKVLNVGVNQADDDLKY
                     LSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNNGHANGKWAAAA
                     ISQNGHMWDAMPGSYYKMG"
BASE COUNT          110 a           66 c          105 g           83 t
ORIGIN      
        1 cttcgtgtgt tgcgctgagt gcctgttctg cacaggaaat tgcagagctg aacaggcaga
       61 taagtgacgg tgcatttgct gtgacaaaag tactgaacgt aggtgtaaac caggcagatg
      121 atgatctcaa atatctgtct caggcacccc gggcaaaaag agagaaacgt gatcatgtca
      181 tagaagtccc tgttgatgtg gatacagctg cgggaagact gaaacggtac tataagtttt
      241 attcaaatga agaagtcgat gcgattcgta ataacggaca tgcgaacggg aagtgggccg
      301 cagcagcaat aagtcagaac gggcatatgt gggatgccat gcccggtagt tattataaaa
      361 tggg
//
LOCUS       DAZGCQ010000467          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000467 DAZGCQ010000000
VERSION     DAZGCQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.473"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..>363
                     /gene="tli1"
                     /locus_tag="ACHK07_005565"
     CDS             49..>363
                     /gene="tli1"
                     /locus_tag="ACHK07_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO8105711.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWSDHLGATESYYNK
                     GKA"
BASE COUNT          106 a           64 c           90 g          103 t
ORIGIN      
        1 atactaaagt tatgatgtct ggtgtttaac gctacaggat gacaatacat gaccagagat
       61 aaaatcattt ttatcgcacc attattggca ttgttaactg ggtgtaattc tgttagctca
      121 cagtatgctg attcggataa gactgaatgg ggttataaaa aattacccta tgatgaatgg
      181 aaatttctct ttatttatcc aaaggctctg cctgcgctgg ccactcaggc attgatggtg
      241 gacggggaag gttataagac gacttatcag tatcttgata ccacaagacc gagtcaggtc
      301 agtgtagggc gctggagtga ccatctgggc gcgactgagt cgtattataa caaggggaaa
      361 gca
//
LOCUS       DAZGCQ010000468          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000468 DAZGCQ010000000
VERSION     DAZGCQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.474"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          118 a           73 c           69 g           97 t
ORIGIN      
        1 tagatgttat gcagtacgca caaaaaaaac aggctggaat tgcttgctga tttataatct
       61 ttatctaaat tactattggc gctttggata ataacataag catatattac ctcctagcaa
      121 taatagagat aacctcatat caaggtgtca ctggtaaata atcggataaa agacagtatc
      181 ggtcggcgca tagagcttct tctatttcac cagaagtgca cagcaggaac taccaatcac
      241 acggttagtg tcatgggtgg aggatgcggc ccgttctttc cctataacag ggttacgcag
      301 tttcaatcag tacatgcaga aaaaccccta attcacatca ctgcagaaag catttaa
//
LOCUS       DAZGCQ010000469          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000469 DAZGCQ010000000
VERSION     DAZGCQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.475"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..224
                     /locus_tag="ACHK07_005566"
     CDS             <1..224
                     /locus_tag="ACHK07_005566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105712.1"
                     /translation="ASRRNVAGGKYPRTLVMNGCFYISCFLMNCVSVVNIHGGKFSGQ
                     KNPPVSSGSTASCFWWKWYGYEVLLLCMP"
BASE COUNT           91 a           64 c           83 g          110 t
ORIGIN      
        1 ccgccagtcg caggaatgtc gctggcggta aatacccacg aacccttgta atgaatggat
       61 gtttttacat cagttgtttt ttaatgaatt gcgtttctgt tgtcaacatt catggtggga
      121 agttttcagg acaaaaaaat ccaccagtaa gctcagggag tactgcgtca tgcttctggt
      181 ggaaatggta cggctatgag gttttattgt tatgcatgcc ataatttttc tacacaaaag
      241 caattctgaa tgtcaatacg ttttataagg aattgttgtg cacacagagt aaatacacgg
      301 ggctgtcagt gtaacactgg cggccttttt catgttgtga gcttccgg
//
LOCUS       DAZGCQ010000470          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000470 DAZGCQ010000000
VERSION     DAZGCQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.476"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          119 a           63 c           54 g          109 t
ORIGIN      
        1 aatcaccttg ataaaattta ataaaatctc tttattttaa cctcgacaat aaaagcggct
       61 ataaaaaaac gctcattttc tgatgctgcg gttaacctgt ggaatgtgcc gttaaccccc
      121 taaaaattgc aaatccaggg tgaacaccct gggttaaaat ggaaataatg agctattttt
      181 tacagtattc accggtaata taagtaacac ttgttgctaa cctaactagc agtacggtta
      241 attaacaaca tagaagttat tgtttttcaa acaactcaat attgcacgcc ggaggattta
      301 tggtcgctaa attaaaacca gatgtttttg ttaatactaa tcctt
//
LOCUS       DAZGCQ010000471          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000471 DAZGCQ010000000
VERSION     DAZGCQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.477"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          107 a           69 c           62 g          104 t
ORIGIN      
        1 agctttgaga catctctatg agtctcaaaa cagttaaaaa ataaaaatta tttttacccg
       61 gtcactttgt aaaaaaaagt gaccgggttt tttgatttct gtgtttcgtg cgcttaatcg
      121 agtctcacgc ttcagtgagc aaattgcccc tccccaacag acatcacgat tcacaaggtc
      181 aaaaattctg ttccatgaag taccgtacaa agataaagag aaaaatgcct tatgcgggat
      241 gtcgctgtcg cgtctttatc gggaaactat acgtaattgc cactttgtct tcggtaatca
      301 gaaaaagaag acctgccatt ttaataaata ttatttctct gg
//
LOCUS       DAZGCQ010000472          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000472 DAZGCQ010000000
VERSION     DAZGCQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.478"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>338)
                     /gene="wzx"
                     /locus_tag="ACHK07_005567"
     CDS             complement(<1..>338)
                     /gene="wzx"
                     /locus_tag="ACHK07_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061350264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O27 family O-antigen flippase"
                     /protein_id="HFO8105713.1"
                     /translation="TSYVAKYIGPSNFGILTYVASIFIIAQALSTFGSEHVLFKRIAK
                     NRKSGINLMMSTSYLRHLVFISLSIGILCYLWVSSDQLTFTFGVATAVAYYFSAIDVF
                     SVYNNAVLNS"
BASE COUNT          128 a           57 c           51 g          102 t
ORIGIN      
        1 tttgaattta atacggcatt attatataca gaaaaaacat caattgctga gaaataatat
       61 gcaactgctg ttgcgacgcc aaatgtaaag gttaattgat cagaactaac ccataaatag
      121 cacaggatcc cgatagataa actaataaaa accagatgtc ttagatagct tgttgacatc
      181 attaaattaa tcccggactt cctatttttt gcaatccttt tgaataaaac atgttcgctt
      241 ccaaaagtag ataacgcttg ggctataata aaaatcgaag ctacatatgt taatatacca
      301 aaatttgaag ggcctatata ttttgccaca taagatgt
//
LOCUS       DAZGCQ010000473          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000473 DAZGCQ010000000
VERSION     DAZGCQ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.479"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>326)
                     /locus_tag="ACHK07_005568"
     CDS             complement(<1..>326)
                     /locus_tag="ACHK07_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105714.1"
                     /translation="EVTRQAAAGSVAVGSHARAGRANTGTYALNGQAVAGRTGSDTAV
                     VSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLS
                     YRVEEL"
BASE COUNT           71 a          105 c           81 g           69 t
ORIGIN      
        1 ttaagctctt ccacccggta gctcagctga ctgaatttat tcccgagagt gtttatcgcc
       61 tgtgtatttc cggcaacctg acggttagtt gcatgcaact gactgccatt gacggcatcc
      121 gttgaattcg cagacaacac acccggtgcc actgactgca gctgacgctc ctgtcccggc
      181 tgaccaaaag aaaccaccgc agtatcactt ccggtacgtc ccgcaacagc ctgaccgttc
      241 agggcataag tcccggtatt cgcgcggcca gcacgggcat ggctgccaac tgcaacagag
      301 ccggcagcag cctgacgcgt gacttc
//
LOCUS       DAZGCQ010000474          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000474 DAZGCQ010000000
VERSION     DAZGCQ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.480"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>325
                     /locus_tag="ACHK07_005569"
     CDS             <1..>325
                     /locus_tag="ACHK07_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105715.1"
                     /translation="GALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQV
                     TWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQ
                     RPVME"
BASE COUNT           79 a           73 c           96 g           77 t
ORIGIN      
        1 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
       61 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
      121 ttcaggagca ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
      181 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
      241 ccggtataca acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca
      301 tcaggcaacg ccccgtcatg gaata
//
LOCUS       DAZGCQ010000475          315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000475 DAZGCQ010000000
VERSION     DAZGCQ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.481"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>315
                     /locus_tag="ACHK07_005570"
     CDS             <1..>315
                     /locus_tag="ACHK07_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8105716.1"
                     /translation="ETAKIPEIPGTHKIPATPKIPVTPQIPVTPQIPEIPETPKVILT
                     GAILNYSDLSGKDLSEYNLTGIICMYTNFSNANLTNCKISNANFSNAKFYNTNCTGAN
                     CS"
BASE COUNT          121 a           66 c           38 g           90 t
ORIGIN      
        1 ctgaaactgc taaaatacct gaaataccgg gaacacataa aatacctgca acgcctaaaa
       61 tacctgtaac acctcaaata cctgtaacac ctcaaatacc tgaaatacct gaaacaccta
      121 aagtaattct tactggagca atactaaatt attcagatct atccgggaaa gatctttcag
      181 aatataatct tactggtatt atctgcatgt ataccaactt ttcaaacgct aatttaacaa
      241 attgtaaaat atctaatgca aacttttcga atgcaaaatt ctacaatact aattgtactg
      301 gtgcaaattg ttcgg
//
LOCUS       DAZGCQ010000476          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000476 DAZGCQ010000000
VERSION     DAZGCQ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.482"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>314
                     /gene="ydjO"
                     /locus_tag="ACHK07_005571"
     CDS             <1..>314
                     /gene="ydjO"
                     /locus_tag="ACHK07_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO8105717.1"
                     /translation="YIHPVGSIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGS
                     GILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAA
                     ME"
BASE COUNT           91 a           55 c           63 g          105 t
ORIGIN      
        1 tatatacatc ctgtcgggag catttttgct gcgattcaaa aaaatgagag tctcctaaag
       61 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
      121 ggaatgggaa gtggtatact tgaagatatt atgagtattc cagatagttc acttagaaaa
      181 cgtctcgggt atgaggtttt aagcttctcg ctacaggcac attcattgag ccaggaatgt
      241 attgataagt tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc
      301 gccgcgatgg aaca
//
LOCUS       DAZGCQ010000477          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000477 DAZGCQ010000000
VERSION     DAZGCQ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.483"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHK07_005572"
     CDS             <1..>311
                     /locus_tag="ACHK07_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105718.1"
                     /translation="ISSEQPLVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGDNVVW
                     KDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAASSGQ
                     AA"
BASE COUNT           81 a           67 c           96 g           67 t
ORIGIN      
        1 ataagcagtg agcagcctct tgttctgaca aatgtggcgg ccggaaatgt gtcaccggta
       61 tccacggatg cggttaacgg gagccagctg gcatctgcag ccagaattgc tggcggtgat
      121 aatgttgtct ggaaagacgg aaaagcagcg ctggctcctg acaccttcag tgagctgaca
      181 actgcaggtg gcggtaagac aacggtgaat ggtggtgtac ataaaacacc ggataatgtg
      241 gctgatgcag tgaatatgct gaaccgtgaa ggcacgaagt attttaaagc tgcctcttcc
      301 ggtcaggcag c
//
LOCUS       DAZGCQ010000478          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000478 DAZGCQ010000000
VERSION     DAZGCQ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.484"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHK07_005573"
     CDS             <1..>311
                     /locus_tag="ACHK07_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8105719.1"
                     /translation="LGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARL
                     PYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQASHPL
                     R"
BASE COUNT           67 a           90 c           79 g           75 t
ORIGIN      
        1 ctgggcttta ttccgctctc ttactacaag aaccgggatt atgcctgctt cttctccgct
       61 aactccaccc agaaaccggc actgtacgat acgccggatg caacggcgaa cagccgtatt
      121 aatgcccgtc ttccctatat cttcctgctg tcccgcattg cgcattacct gaagatgatt
      181 cagcgggaaa atatcggtac caccaaagat cgccgtctgc ttgagctgga gctgaataac
      241 tggatccggg gtctggtgac ggagatgacc gatccgggtg atgagttgca ggcctcgcat
      301 ccgctgcgtg a
//
LOCUS       DAZGCQ010000479          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000479 DAZGCQ010000000
VERSION     DAZGCQ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.485"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..191
                     /locus_tag="ACHK07_005574"
     CDS             <1..191
                     /locus_tag="ACHK07_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105720.1"
                     /translation="NLKKKGILYKEKARKTPLCETIVLNLRYGGKTRYRLFSCRRHPN
                     GFRVHRNGGAFIIQTQNN"
BASE COUNT          115 a           65 c           67 g           61 t
ORIGIN      
        1 ataacctgaa aaaaaaaggt atactatata aagaaaaggc aaggaagacg ccactctgtg
       61 aaactattgt cctgaatctg aggtatgggg gaaaaacccg ctacagactg ttctcctgca
      121 ggaggcatcc caatggattc agagtacatc gcaatggcgg agcattcatt attcagacgc
      181 agaataatta atctcatttt ttcaggagca gatatagcag aataaaactg aacacagacc
      241 agcaaagccc gactggatct ttagcccgca aagaaaaagc ggcaatcccg gaaaaataac
      301 agccggat
//
LOCUS       DAZGCQ010000480          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000480 DAZGCQ010000000
VERSION     DAZGCQ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.486"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>308
                     /locus_tag="ACHK07_005575"
     CDS             <1..>308
                     /locus_tag="ACHK07_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8105721.1"
                     /translation="FLISYRPNWRWLGMRETAAKSFVDEVEAAWSEYAEGMSGEIDVE
                     GKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNR
                     "
BASE COUNT           68 a           65 c          108 g           67 t
ORIGIN      
        1 tttctgatca gctaccgtcc gaactggcgc tggctgggga tgcgggagac tgcggcaaaa
       61 agttttgtcg atgaggtgga ggcggcctgg tcggaatacg ccgaagggat gtctggcgag
      121 atcgacgtgg aaggaaaacg cacgttcacg gaatttatcc gtgaaggtgt gggcgttcat
      181 gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata cggagagcac gcagctgttt
      241 cgtacacgtt ttaaagccgt gagtccgaaa cgggtggaca cgccaggaca cggtatgggg
      301 aaccgttt
//
LOCUS       DAZGCQ010000481          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000481 DAZGCQ010000000
VERSION     DAZGCQ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.487"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          102 a           57 c           49 g           99 t
ORIGIN      
        1 aaatgaagta actctcgaaa gaatgattac agccattaag tcacatttta aatattattc
       61 tgtctactgg aatagtggta atttctattc aatagctgcc agcaattcgc aaaaaacatt
      121 tctgacttta taatgttgct tcctgaggca gccataccta tggagtatgt ttatcttaag
      181 gttccaacca gaataacagg agttatataa tcccatggac ttctcaacat taataacatt
      241 ctctattata accttatcac aaacagtatc gattggtccg ggggtggcat taatcatatc
      301 tgaaagt
//
LOCUS       DAZGCQ010000482          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000482 DAZGCQ010000000
VERSION     DAZGCQ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.488"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHK07_005576"
     CDS             <1..>306
                     /locus_tag="ACHK07_005576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8105722.1"
                     /translation="KEAADEAAANADKDQNGLITPAEAKAVEDANTALDTAKQAAQEA
                     VNKVPEGTEGKEGLQTRLDGLTPADVPAVTDQDGNGKADAAEASLAAAEAAVQAAEAK
                     "
BASE COUNT           82 a           74 c          115 g           35 t
ORIGIN      
        1 aaagaggcag ccgatgaagc cgccgcgaat gcagacaagg atcagaacgg tctgatcacg
       61 ccggcagagg cgaaagccgt ggaagatgcc aatacggcgc tggatacggc gaagcaggca
      121 gcccaggagg cggtgaacaa ggtaccggaa ggtacggaag gtaaggaagg tctgcagaca
      181 cgtcttgatg gtctgactcc ggcggatgta ccggcggtga cggatcagga cggtaacggc
      241 aaggcggatg cagcagaagc gtccctggct gcagcggaag ccgccgttca ggcagcggaa
      301 gcgaag
//
LOCUS       DAZGCQ010000483          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000483 DAZGCQ010000000
VERSION     DAZGCQ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.denovo.489"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..>303)
                     /gene="yddG"
                     /locus_tag="ACHK07_005577"
     CDS             complement(14..>303)
                     /gene="yddG"
                     /locus_tag="ACHK07_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO8105723.1"
                     /translation="VYYFLTPQPEMVFSMPVMIKLISAAFTLGFAYAAWNVGILHGNV
                     TIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGALMVCGGSLLCWLATRRG"
BASE COUNT           89 a           79 c           81 g           54 t
ORIGIN      
        1 cccggcaatt attttagcca cgacgtgtcg ccagccagca gagcagggaa ccgccgcaga
       61 ccatcagcgc gccttgccag aacgagaacg acagcggggc actgagcagc acggctgcaa
      121 gcgctgagga aagtacgggc gtaaaatacg aacctaccgc cattatggtg acgttgccat
      181 gcagtatacc gacattccat gcagcataag caaatcctaa ggtaaatgcc gcagagatga
      241 gtttaatcat gacgggcatg ctaaatacca tttctggttg tggcgtgaga aaatagtaaa
      301 ccc
//
LOCUS       DAZGCQ010000484         4993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.221, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000484 DAZGCQ010000000
VERSION     DAZGCQ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.221"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..445
                     /locus_tag="ACHK07_005578"
     CDS             2..445
                     /locus_tag="ACHK07_005578"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8105724.1"
                     /translation="MQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDES
                     RWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIKSES
                     ELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            636..844
                     /locus_tag="ACHK07_005579"
                     /pseudo
     CDS             636..844
                     /locus_tag="ACHK07_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            864..1172
                     /locus_tag="ACHK07_005580"
                     /pseudo
     CDS             864..1172
                     /locus_tag="ACHK07_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1229..2686)
                     /gene="pepD"
                     /locus_tag="ACHK07_005581"
     CDS             complement(1229..2686)
                     /gene="pepD"
                     /locus_tag="ACHK07_005581"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8105725.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2947..3405
                     /gene="gpt"
                     /locus_tag="ACHK07_005582"
     CDS             2947..3405
                     /gene="gpt"
                     /locus_tag="ACHK07_005582"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8105726.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCIYSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3497..4741
                     /gene="frsA"
                     /locus_tag="ACHK07_005583"
     CDS             3497..4741
                     /gene="frsA"
                     /locus_tag="ACHK07_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8105727.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4799..>4993
                     /gene="crl"
                     /locus_tag="ACHK07_005584"
     CDS             4799..>4993
                     /gene="crl"
                     /locus_tag="ACHK07_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8105728.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQES"
BASE COUNT         1217 a         1245 c         1273 g         1258 t
ORIGIN      
        1 catacaaata agaaactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
       61 agcggttacg atgacagcca gtcagcatta ttcaccacaa caaattgctg cctgggcgca
      121 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
      181 taatgcacaa ccggttggtt ttatttcccg cattgaacat tatatcgata tgttatttgt
      241 tgaccctgaa tacacccgcc gtggggttgc cagtgctttg ttaaaacctt ggattaagtc
      301 tgaatccgaa cttacggtgg acgccagcat aaccgcaaaa cccttttttg aacgttatgg
      361 ttttcagaca gttaagcagc agcgcgttga atgccgggga gcgtggttta ctaattttta
      421 tatgcgatat aaaccgcaac attaaatcca gcttgcaatg aaaataacgc ccgcctggta
      481 tgtgcgcgtt ttctgattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
      541 atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc
      601 catttcctga tttttattca taaatggaat ggtacatggg caaatatatt cgtcccttat
      661 ccgatgcggt atttaccatc gcatctgatg acctgtggat cgagagttta gcgatccaac
      721 aattacacac cacggcaaat ttacccaaca tgcagcgcgt agtggggatg ccagatttac
      781 acaccccgga cgcggctacc cgattggcgc agcgttcttc tctgttggtc gtttttaccc
      841 ggcaagacgt cgcggtaacg gtgctggaaa ccgaagccgg gcgttatcct gacacgctgc
      901 gttcgtcggg gccgggcggt caaaatgtca ataaaaccga cttggcggta cgcgccacgc
      961 atctggcatc cggtattagc gtgaaggttc agtcagagcg cagccagcat gctaacaaac
     1021 ggctggcgcg attgctgatt gcctggaggc tggagcagca gcaacaggaa aatagcgcgg
     1081 cgctgaaatc acagcggcga atgttccatc accagattga acgtggcaac ccgcgacgaa
     1141 cgtttacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt
     1201 aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag
     1261 tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg
     1321 gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca
     1381 gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg aacagacgct
     1441 gataggtttc acgtaccaga tgcatcaccg gagagtttgc gtccggctgc cagccaggat
     1501 atgcaccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca
     1561 ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacattat
     1621 tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt
     1681 cggaattgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag
     1741 atttcgcaat cagggcagct ttatcgttcg caacagagtc cagcaacaag gccagatttt
     1801 tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca
     1861 gggcgtcgac tttatcagcc gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt
     1921 tacgcagtgt gccgccgttg aaatcaataa gacgcagatc cagttcttct gcatgacccg
     1981 ccaggaagcg caccagcagt ttgttggcat taccgaggcc aacgtggatt tcaccgccgg
     2041 agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaacct
     2101 cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa cccatgtaga
     2161 tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct
     2221 gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg
     2281 ggccgtgaac cacgttttcg tcagccagaa ccgccagcgc agaggccata ccaatgccgt
     2341 tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga
     2401 taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt
     2461 gggcctgcaa gacgaccggt ttacgatttt ccatacctgc tgtagcaggt ttacgaatca
     2521 agatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
     2581 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
     2641 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
     2701 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
     2761 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     2821 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     2881 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     2941 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     3001 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     3061 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     3121 tgtatttaca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     3181 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     3241 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa accggctggt
     3301 cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat cgaacagccg
     3361 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     3421 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     3481 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     3541 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     3601 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     3661 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     3721 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     3781 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga
     3841 agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc
     3901 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg
     3961 cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     4021 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     4081 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     4141 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     4201 ttcatcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc
     4261 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     4321 cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg
     4381 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     4441 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg atgaagcgtt
     4501 gcgcgtggag ctgaatcgtt attcattaaa agtgcaagga ttgctgggac gtcgctgccc
     4561 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     4621 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     4681 tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac gcttgtgtta
     4741 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
     4801 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     4861 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     4921 cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga
     4981 agcgcaggaa tcc
//
LOCUS       DAZGCQ010000485         4993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.222, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000485 DAZGCQ010000000
VERSION     DAZGCQ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.222"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..445
                     /locus_tag="ACHK07_005585"
     CDS             2..445
                     /locus_tag="ACHK07_005585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8105729.1"
                     /translation="MQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQIDES
                     RWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIKSES
                     ELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            636..844
                     /locus_tag="ACHK07_005586"
                     /pseudo
     CDS             636..844
                     /locus_tag="ACHK07_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            864..1172
                     /locus_tag="ACHK07_005587"
                     /pseudo
     CDS             864..1172
                     /locus_tag="ACHK07_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1229..2686)
                     /gene="pepD"
                     /locus_tag="ACHK07_005588"
     CDS             complement(1229..2686)
                     /gene="pepD"
                     /locus_tag="ACHK07_005588"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8105730.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2947..3405
                     /gene="gpt"
                     /locus_tag="ACHK07_005589"
     CDS             2947..3405
                     /gene="gpt"
                     /locus_tag="ACHK07_005589"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8105731.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3497..4741
                     /gene="frsA"
                     /locus_tag="ACHK07_005590"
     CDS             3497..4741
                     /gene="frsA"
                     /locus_tag="ACHK07_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8105732.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4799..>4993
                     /gene="crl"
                     /locus_tag="ACHK07_005591"
     CDS             4799..>4993
                     /gene="crl"
                     /locus_tag="ACHK07_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8105733.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQES"
BASE COUNT         1216 a         1246 c         1273 g         1258 t
ORIGIN      
        1 catacaaata agaaactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
       61 agcggttacg atgacagcca gtcagcatta ttcaccacaa caaattgctg cctgggcgca
      121 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
      181 taatgcacaa ccggttggtt ttatttcccg cattgaacat tatatcgata tgttatttgt
      241 tgaccctgaa tacacccgcc gtggggttgc cagtgctttg ttaaaacctt ggattaagtc
      301 tgaatccgaa cttacggtgg acgccagcat aaccgcaaaa cccttttttg aacgttatgg
      361 ttttcagaca gttaagcagc agcgcgttga atgccgggga gcgtggttta ctaattttta
      421 tatgcgatat aaaccgcaac attaaatcca gcttgcaatg aaaataacgc ccgcctggta
      481 tgtgcgcgtt ttctgattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
      541 atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc
      601 catttcctga tttttattca taaatggaat ggtacatggg caaatatatt cgtcccttat
      661 ccgatgcggt atttaccatc gcatctgatg acctgtggat cgagagttta gcgatccaac
      721 aattacacac cacggcaaat ttacccaaca tgcagcgcgt agtggggatg ccagatttac
      781 acaccccgga cgcggctacc cgattggcgc agcgttcttc tctgttggtc gtttttaccc
      841 ggcaagacgt cgcggtaacg gtgctggaaa ccgaagccgg gcgttatcct gacacgctgc
      901 gttcgtcggg gccgggcggt caaaatgtca ataaaaccga cttggcggta cgcgccacgc
      961 atctggcatc cggtattagc gtgaaggttc agtcagagcg cagccagcat gctaacaaac
     1021 ggctggcgcg attgctgatt gcctggaggc tggagcagca gcaacaggaa aatagcgcgg
     1081 cgctgaaatc acagcggcga atgttccatc accagattga acgtggcaac ccgcgacgaa
     1141 cgtttacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt
     1201 aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag
     1261 tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg
     1321 gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca
     1381 gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg aacagacgct
     1441 gataggtttc acgtaccaga tgcatcaccg gagagtttgc gtccggctgc cagccaggat
     1501 atgcaccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca
     1561 ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacattat
     1621 tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt
     1681 cggaattgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag
     1741 atttcgcaat cagggcagct ttatcgttcg caacagagtc cagcaacaag gccagatttt
     1801 tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca
     1861 gggcgtcgac tttatcagcc gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt
     1921 tacgcagtgt gccgccgttg aaatcaataa gacgcagatc cagttcttct gcatgacccg
     1981 ccaggaagcg caccagcagt ttgttggcat taccgaggcc aacgtggatt tcaccgccgg
     2041 agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaacct
     2101 cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa cccatgtaga
     2161 tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct
     2221 gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg
     2281 ggccgtgaac cacgttttcg tcagccagaa ccgccagcgc agaggccata ccaatgccgt
     2341 tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga
     2401 taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt
     2461 gggcctgcaa gacgaccggt ttacgatttt ccatacctgc tgtagcaggt ttacgaatca
     2521 agatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
     2581 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
     2641 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
     2701 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
     2761 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     2821 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     2881 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     2941 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     3001 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     3061 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     3121 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     3181 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     3241 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa accggctggt
     3301 cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat cgaacagccg
     3361 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     3421 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     3481 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     3541 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     3601 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     3661 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     3721 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     3781 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga
     3841 agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc
     3901 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg
     3961 cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     4021 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     4081 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     4141 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     4201 ttcatcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc
     4261 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     4321 cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg
     4381 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     4441 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg atgaagcgtt
     4501 gcgcgtggag ctgaatcgtt attcattaaa agtgcaagga ttgctgggac gtcgctgccc
     4561 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     4621 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     4681 tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac gcttgtgtta
     4741 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
     4801 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     4861 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     4921 cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga
     4981 agcgcaggaa tcc
//
LOCUS       DAZGCQ010000486         4065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.238, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000486 DAZGCQ010000000
VERSION     DAZGCQ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.238"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..541
                     /locus_tag="ACHK07_005592"
     CDS             <1..541
                     /locus_tag="ACHK07_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HFO8105734.1"
                     /translation="AAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIY
                     RTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMA
                     RKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGG
                     EADAADKAEAANKKEKANG"
     gene            534..1268
                     /gene="frdB"
                     /locus_tag="ACHK07_005593"
     CDS             534..1268
                     /gene="frdB"
                     /locus_tag="ACHK07_005593"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8105735.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1279..1674
                     /gene="frdC"
                     /locus_tag="ACHK07_005594"
     CDS             1279..1674
                     /gene="frdC"
                     /locus_tag="ACHK07_005594"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8105736.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1685..2044
                     /gene="frdD"
                     /locus_tag="ACHK07_005595"
     CDS             1685..2044
                     /gene="frdD"
                     /locus_tag="ACHK07_005595"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8105737.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2107..3240
                     /locus_tag="ACHK07_005596"
     CDS             2107..3240
                     /locus_tag="ACHK07_005596"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8105738.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            3329..3862
                     /gene="blc"
                     /locus_tag="ACHK07_005597"
     CDS             3329..3862
                     /gene="blc"
                     /locus_tag="ACHK07_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8105739.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3859..>4065)
                     /locus_tag="ACHK07_005598"
     CDS             complement(3859..>4065)
                     /locus_tag="ACHK07_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFO8105740.1"
                     /translation="AMIVSMALLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGES
                     ANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT          997 a         1074 c         1081 g          913 t
ORIGIN      
        1 agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca
       61 ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcta tggaagaagg
      121 ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggctgagct
      181 gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct
      241 gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc
      301 aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg
      361 tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg
      421 cctggagtac agcgacgtga agattactac gctaccgcca gctaaacgcg tttacggtgg
      481 cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg
      541 agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc
      601 atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct
      661 acatcaaaga caacctggca ccggacctga gctaccgctg gtcctgccgt atggcgattt
      721 gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc
      781 gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc
      841 tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg
      901 gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag atggcgaagt
      961 atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg
     1021 gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag
     1081 atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat
     1141 ggagctgtac tttcgtgggc tactgctccg aagtctgccc gaaacacgtc gatccggctg
     1201 cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac
     1261 cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc
     1321 acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt
     1381 ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg
     1441 gaagcctggg cgggattcgt cgacttttta caaaacccgg ttattgtgat cattaacctg
     1501 atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg
     1561 gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg
     1621 gcggtaactg tagttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc
     1681 tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg
     1741 ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg ttggtattct
     1801 gctgccgctg gggctgtttc cgggtgatgc actgagctac gagcgcgttc tggcgttcgc
     1861 gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg
     1921 tttacaccgt atgcaccacg cgatgcacga tctgaaaatc cacgtacctg cgggcaaatg
     1981 ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggtg tcgttacaat
     2041 ctaacgcatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca
     2101 gaccctatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt
     2161 gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa
     2221 caaaagatcc cgggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc
     2281 tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta
     2341 ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc tcgaggggaa
     2401 atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaac
     2461 gggatcacgc tattacatct cgcaacctac accgctggcg gcctgccatt gcaggtgccg
     2521 gatgaggtga aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg
     2581 gcgccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct
     2641 gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc
     2701 aaactcaacc atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga
     2761 tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga aacttatggt
     2821 gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat
     2881 atcaacgaca aaacacttca gcaagggata caactggcac aatctcgcta ctggcaaacc
     2941 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtaaa tcctgacagc
     3001 atcattaacg gcagtgacaa taaaattgca ctggcagcac gccccgtaaa agcgattacg
     3061 cctccaactc ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt
     3121 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa
     3181 aactatccca atccagcgag agtcgccgcc gcctggcaga ttctcaacgc tctgcagtaa
     3241 aatttcatcg ggtccgaatt ttcggacctt ttctccgctt ttccttgctg tcatctacac
     3301 ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
     3361 agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgaccgtagt
     3421 gaataatttc gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca
     3481 ccgctttgag cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg
     3541 cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg
     3601 gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc
     3661 tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg
     3721 cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt
     3781 gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta aatttatttg
     3841 ggtacagcag cccggcagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat
     3901 taacgccaga ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt
     3961 gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa
     4021 cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgc
//
LOCUS       DAZGCQ010000487         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.316, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000487 DAZGCQ010000000
VERSION     DAZGCQ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.316"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005599"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO8105741.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005600"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO8105742.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          364 a          252 c          303 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZGCQ010000488         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.317, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000488 DAZGCQ010000000
VERSION     DAZGCQ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.317"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005601"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO8105743.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005602"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO8105744.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          253 c          302 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZGCQ010000489         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2206S40850
            SAMN43267920-rid23083503.guided.318, whole genome shotgun sequence.
ACCESSION   DAZGCQ010000489 DAZGCQ010000000
VERSION     DAZGCQ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267920
            Sequence Read Archive: SRR30307998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351314.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 79 of 177
            Pseudo Genes (incomplete)         :: 111 of 177
            Pseudo Genes (internal stop)      :: 37 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267920-rid23083503.guided.318"
                     /serotype="O 27:H30"
                     /isolate="2206S40850"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005603"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK07_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO8105745.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005604"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK07_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO8105746.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          362 a          253 c          303 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
